LOCUS       DAOQSA010000001        90801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_78_length_90801_cov_9.051352, whole genome shotgun sequence.
ACCESSION   DAOQSA010000001 DAOQSA010000000
VERSION     DAOQSA010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 90801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 90801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90801
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78_length_90801_cov_9.051352"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1075..2439)
                     /locus_tag="PKK65_00005"
     CDS             complement(1075..2439)
                     /locus_tag="PKK65_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNU81105.1"
                     /translation="MALTSKITKNTKKPVKKVIWKKKVSKPVKKAPVKKVAAPAIKRA
                     VPPKPVKKVIWKKKVSKPVKKAPVKKVAAPAIKTTPQNKPKVALTPREVVRPTEEQLK
                     KFLNKGRLRGFVTETELLYLFPEVEEYIYEYDVFLGELQKNGIRIAEDSGRILDVQEK
                     NPHITSTKAAAAVTFDLSKLSADSIQMYLKEIGKVPLLSGEEEVELAKRKEKGDKEAE
                     KKIIEANLRLVVSIAKKFAGAKGLSLLDLIQEGNIGLFRAVEKFEYRKGYKFSTYATW
                     WIRQAITRALADQSRTIRIPVHMVETINKFQQVQRTLIQELGREPLAEEIASEMGENI
                     EKVRYIIKISQDTISLETSIGDDEEDSTLEDFIEDVKNITPDRAAALQLLRDYVKNIV
                     AQLSPREQKILEMRFGLIDGVAHTLEEVGQEFEVTRERIRQIESKALEKIRKLKGLEK
                     LKDY"
     gene            complement(2444..4249)
                     /gene="dnaG"
                     /locus_tag="PKK65_00010"
     CDS             complement(2444..4249)
                     /gene="dnaG"
                     /locus_tag="PKK65_00010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNU81106.1"
                     /translation="MSPAEEIKAKLDIVEVIREYLQVKAVGVNFQALCPFHREKSPSF
                     VISPDKQIWHCFGCGKGGDVLSFVQEMEGLSFPDTLRLLAPKAGVVLRQAAAGEYSKR
                     GRLLDCLESAARQYVNYLENDATGRRMKAYLLKRGLSENTIKDWQIGYGSEQWTAMFD
                     YLRSQKFTEQEIFAAGLAGRSERGSVYDRFRDRIIFPIREASGTLIAFTARVNPEKAD
                     TVTGGKYINSPQTEVYDKSRVLFALDKAKKAIKERGFAIVVEGQMDAIACHQHGFTNT
                     VASSGTALTREQLKLLKRFSNNLILSFDMDSAGQLAADRGIKEALALDMNIKIIVLPS
                     QYKDPDECLRANPDDFKQAVINSRPMMEYYFEKVKSGKDLRKLSDKKEVARLMLAMIA
                     KFSNKIDRDYWLQRLAENLGISEQALRESLPVVQVNRPELTATKATLTPTAPVLGLTR
                     EERLSEILLALLLKAPDFITYSVSHLEPDDLSGESLIAFYKNLIIYYNKFAVLDYDSF
                     RLYLQEQADGSAEIIDRLALLGEKDYYQHDANQLKAEITKVLLELKRFSLKRQIKSFQ
                     DRISAAEAAGESEGMTMLMEEIKNLTEQLNSLHSL"
     gene            complement(4253..4684)
                     /locus_tag="PKK65_00015"
     CDS             complement(4253..4684)
                     /locus_tag="PKK65_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81107.1"
                     /translation="MKIIIDNQRLGGASPAQWLRRAGYTLINDRQRGQESFVRRLSRD
                     FYPRFHLYVQEREGGGYFFNLHLDHKKASYEGQSRHSADYDGEMVEQEAARLSSLLLP
                     PSASSAPIAGGNIQADVLTTLKPSFKPPLAPKRSWWQRLFS"
     gene            complement(4686..5150)
                     /locus_tag="PKK65_00020"
     CDS             complement(4686..5150)
                     /locus_tag="PKK65_00020"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNU81108.1"
                     /translation="MSLINRFYDDFRNFFAHHFPKVFAFCNRRKALVKFFISGGIAGA
                     VDLAVLFILHGLMGWGILLATFWAFLISFVVSFSLQKLWTFRNYSHKHLPHQLIISFS
                     AAFISLNLNGLGMHILVNNLHVWYLFSQILVNIFLGVINFIIYNFIIFRQTK"
     gene            complement(5155..6066)
                     /locus_tag="PKK65_00025"
     CDS             complement(5155..6066)
                     /locus_tag="PKK65_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNU81109.1"
                     /translation="MLNLIANLLALSLSVWSPPVNIVYSSGPLRGTEACLESGHLPLN
                     DDVPARKMPAPSIAAKSAAVLTDDGHVWLAAKAPERRQAIASITKLMTALVFLDNNPG
                     WEKEYTIRRDDIVEGGKINLFLGETVKVKDLFNASLVASDNGATLALVHATGLSTADF
                     IVAMNNKAKDLGLLQTEFADPVGLSNGNLSNVRDVARLAQAALNKAEIAAAVARHTYR
                     FQTVQGREKLLESTDWLLDSSSSSTLKALGGKTGYTDQAGYCFVGRFQDKDGRSVIVA
                     VLDTGGKNERFLQARALATWAFTYCQW"
     gene            complement(6060..6611)
                     /gene="scpB"
                     /locus_tag="PKK65_00030"
     CDS             complement(6060..6611)
                     /gene="scpB"
                     /locus_tag="PKK65_00030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNU81110.1"
                     /translation="MSSLSSQLESLLLVANKPLSLKELSAATGIKAAAVEEALLALSR
                     EYEESGRGWRLASAANNYQLVSAPDNSDLIKAFLKAETSGEWSQPSLEALTIIAYRGP
                     VSKMELDRIRGVNCSLIIRNLLLRGLIEEEFDKKKNENYYQVTLDFIRFLGLNNVTEL
                     PDYERLHQPEELKAFLAESEKAC"
     gene            complement(6661..7380)
                     /locus_tag="PKK65_00035"
     CDS             complement(6661..7380)
                     /locus_tag="PKK65_00035"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNU81111.1"
                     /translation="MIDFQVEKFQGPLGLLLQLIESEEMDITEIALAAIADQYVAYVE
                     QAENINPEEIADFLVIAARLLYLKSKALLPYLSSEEEEAEIGDLERQLRMYKEFIEAS
                     LKVSDLINRDHKSFAPIFNKSGRRGLADKELIFAPPKQITASALENIWQQILARLKKQ
                     EEKLPEARLEPKVSLDERISHIRGLLAEQLSLSFQRLLEKAETKVEVIVNILAVLELA
                     KQRELVFEQEELFSEIRVLKI"
     gene            complement(7383..8318)
                     /locus_tag="PKK65_00040"
     CDS             complement(7383..8318)
                     /locus_tag="PKK65_00040"
                     /inference="COORDINATES: protein motif:HMM:NF039189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HNU81112.1"
                     /translation="MTNNHNAKYAFYYLLSLVSLIFTALSVGMIAFGIIDKSVADAWN
                     YNSYNNLDSQLKFAISALFIAAPIYYFLSHLINRGLNKQELDLNSPLRRWLTYFIILV
                     TSMIILGVFIGVINNFLSGELTSRFILKAATTLLIAAIIFSFYFYDIKRQEIKAKDLV
                     IRFFAFGSAALVIVAFVSAWFLVTPPSVARAKRLDQNLVNNIYQLENAVNSYYSQYGK
                     LPASLEEIKDSDIYLDSRITVDPATKQAIEYRREEDNKFSFCAHFRLASDESDQNFAY
                     PSVKKGHQAGYDCLKGQLWSKEESDQGAVQPILID"
     gene            complement(8407..9795)
                     /gene="asnS"
                     /locus_tag="PKK65_00045"
     CDS             complement(8407..9795)
                     /gene="asnS"
                     /locus_tag="PKK65_00045"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNU81113.1"
                     /translation="MYTTSKQLYRHTDEFLNKEILVAGWVRTVRSSGDVAFIELNDGS
                     FFKGLQIVINKGLPNFAALEKLNIGSSLEVKGVLVPSPAAGQAFELSAVSVTVINEAK
                     EDYPLQKKKHSFEFLREIAHLRPRSNTFSAVFRLRSALSYAIHKFFQEEEFAYVQTPI
                     ISTSDCEGAGEMFQVTTLDLKKVPMQKNGEIDFGKDFFGQKAGLTVSGQLNAEALIMG
                     LNKVYTFGPTFRAENSNTTRHASEFWMIEPEMAFAELKDDMDLAERMLKYLINYVLTE
                     LPEEMEFLNQFIDNWLIERLKAVAAADFGRVTYTEAIDLLLKSGENFAYPVSWGIDLQ
                     TEHERYLAEKIFKKPIFLTDYPKDIKAFYMRQNDDGKTVAAMDLLVPGIGEIIGGSQR
                     EERYEWLENRIVEMGMNPEDYRWYLDLRQYGSIKHAGFGLGFERAIMYLSGMANIRDV
                     IPFPRTPKNAKF"
     gene            complement(9808..11112)
                     /gene="aspS"
                     /locus_tag="PKK65_00050"
     CDS             complement(9808..11112)
                     /gene="aspS"
                     /locus_tag="PKK65_00050"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF003483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HNU81114.1"
                     /translation="MSKISIKAAKEQALGKVELHGFVQNIRLMRGLVFIDLRDSSGNM
                     QLVVEEKIGASFTAAEALTLESFIKVVGELKEKPAKKNDPNPVKDWELSVSSLEIISL
                     AEANLPIPVLNKIDNEANIDNRFDWRWLDLRRPEHRQIFQVWTQLEVGFREQLLAEGF
                     MQIYTPCFMNTASETGSEVFEVKYFDRQAYLSQSPQFYKQMAIASGFEKVFMTGPVFR
                     AEASYTSRHVTEFTGWDFEFADIESHHDIMALEERTLIRAFGKVKELGLDIEIPAAPF
                     PKMSLAEVKEKLKAAGIKSEKDYDLSPEEEREICRLVKEEFNHDFIFVTDYPIAARPF
                     YHMRHADNPGLTKSFDLLYRGLEVTTGSQREHRLDVLEKQALEKEMNLEELHDYFNFF
                     RYGCPAHGGVGIGPARLVMKIVGADNVKEACFLPRDAKRLRP"
     gene            complement(11263..12000)
                     /gene="recO"
                     /locus_tag="PKK65_00055"
     CDS             complement(11263..12000)
                     /gene="recO"
                     /locus_tag="PKK65_00055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNU81115.1"
                     /translation="MDSTFPTRAIILNRRDWREADRLVSVYTPDYGRLSLVARGARKA
                     GSKLAAHLEPISLSRLLVVKGKGFDYVGAALSEEIFLGIKQDLNKLYFAGEALRNFSI
                     LVQEGEADLNLFSWLEKWLLSLEAAGEKGSLDKDEGRFRLALFLWRLFKLLGHEPRLD
                     VCTACKAAIKPGNNYFDCNRGGLLCPDCYSSNLKISAPDSMVPISDNCIKLLRLASGN
                     TWQNLKFSPKILKEWENLNFQIISWLY"
     gene            complement(12004..12399)
                     /gene="ruvX"
                     /locus_tag="PKK65_00060"
     CDS             complement(12004..12399)
                     /gene="ruvX"
                     /locus_tag="PKK65_00060"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNU81116.1"
                     /translation="MKMEEGKQYLGVDWGEKRIGLALADAETRLALPFMTVTDLRSLL
                     AIIEAESIALVVVGSPRKMAGDEASNPLFLHFLKELKSKSTVAVVTVDERLSSKAADA
                     LAGSKRDKAGRDEIAATIFLQNYLDQENK"
     gene            complement(12401..13234)
                     /gene="map"
                     /locus_tag="PKK65_00065"
     CDS             complement(12401..13234)
                     /gene="map"
                     /locus_tag="PKK65_00065"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNU81117.1"
                     /translation="MVYIKTKEEIEAIRAGGKILASILKRLKAAVKPGVNTADLEGMM
                     LAMVAAAGGRPAFKDYPMGGGIFFPSAICASINDEVVHGAAIPGRILKAGDIIDLDIG
                     MEWPVKPELREKLSLPLNPHSPEGGFYTDMCATVPVGEISREAKRLLRVTRECLEAGI
                     KQVRPGARLNNVGGAVQSLAETAGYGVVRDLVGHGVGYQVHEDPEIYHYRIGERSAAN
                     IELKEGMVICIEPMINAGTYKIEVAENGFTIVTADKSLSAHFEHTLAVTKDAYEILTL
                     E"
     gene            complement(13282..13575)
                     /locus_tag="PKK65_00070"
     CDS             complement(13282..13575)
                     /locus_tag="PKK65_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81118.1"
                     /translation="MDLKDFLANIKDEPSDIIERKLNELVRENYHFQHLSSENKEIVL
                     ALVKRYKEKLRTGKGVSQYVINRDMYKLYRQRYDLKLTEVDCDHIREILESFK"
     gene            complement(13680..14951)
                     /gene="secY"
                     /locus_tag="PKK65_00075"
     CDS             complement(13680..14951)
                     /gene="secY"
                     /locus_tag="PKK65_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNU81119.1"
                     /translation="MLAKLEQIWKAKDLRRSILFVFAMLVVFRLAAHIPVPGVDTAAL
                     KNLFASNEILGLMNIFSGGGMENFSIVMMGIAPYITASIIFQLLGMIIPKIEEMQKEE
                     SGRQKINMWTRWATVPLAAMQAYGMITLLQRSNQGILGDISAFDFGVIILTITAGTIF
                     LMWIGELISEKKIGNGISLLIFAGIVAGIWQQLQQFILTFDQTQIFTVVGFVVVAILT
                     VIGVVIVNEGQRNIPVQYARQIRGNRAFGGTSTHLPLRVNMAGVIPIIFAISVVLFPP
                     MIAQFFLHARTAWIANFSAKVIEIFNNQLIYGIIYFILVFAFTYFYTEVIFHPDQISE
                     NLQKQGGFIPGIRPGKPTRDYLAYTAHRIIFAGALFLSLIAVLPLVMRYFTGIQSMAI
                     GGTGLLIVVSVVIETVKQIESQLTMREYDGI"
     gene            complement(14990..15406)
                     /gene="rplO"
                     /locus_tag="PKK65_00080"
     CDS             complement(14990..15406)
                     /gene="rplO"
                     /locus_tag="PKK65_00080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNU81120.1"
                     /translation="MLSLNNIKRSSGATHKKKRVGRGNASGHGTYSTRGLKGQKARSG
                     VSGLKRLGMRKQLLQTPKLRGFRSLQPKNQIVSVALINKNFHDGDVITPESLWQAGLI
                     KNVSSPVKILGKEKLTIKVKFEKVAMSAGVKSQISA"
     gene            complement(15436..16125)
                     /locus_tag="PKK65_00085"
     CDS             complement(15436..16125)
                     /locus_tag="PKK65_00085"
                     /inference="COORDINATES: protein motif:HMM:NF015664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNU81121.1"
                     /translation="MADDNKQVEADTPENKVATAVAKDIYGGQEKRQRGASGRGPRRD
                     SRGSRDERKDEKEQRILEIARVTRVMAGGKRMNFRACVAIGDRKGNIGLGLGKGADVT
                     LAVNKAVNRAQKEMLKVSIVNDTIPHVVRQKLGAALIVFKPAQQGRGVIAGGVVRAIL
                     ELAGIKNVTSKVLGTNNKINNARCTFAALASLRQPDKKSVKTKEKKEVKTEVKEEKTV
                     KAAPKKAATKK"
     gene            complement(16155..16514)
                     /gene="rplR"
                     /locus_tag="PKK65_00090"
     CDS             complement(16155..16514)
                     /gene="rplR"
                     /locus_tag="PKK65_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNU81122.1"
                     /translation="MMNIIKKEKARKQRRQDKVRAKISGTASRPRLNVFRSNRGLYLQ
                     LIDDVSGRTLASAHVRELKDKKLKKVEAAAALGKILAEKARALGIKEAVFDRSSYRYH
                     GRVKAVAEAAREQGLVF"
     gene            complement(16537..17076)
                     /gene="rplF"
                     /locus_tag="PKK65_00095"
     CDS             complement(16537..17076)
                     /gene="rplF"
                     /locus_tag="PKK65_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNU81123.1"
                     /translation="MSRLGKLPIKLLEGVSAQFNNGVLTVKGPKGELSRALKTPVTVE
                     IDEAAIRIVVTQNTKDASAYWGLYRALVNNMVTGVKVGFTKKLEINGVGYRVAVNGSK
                     LNLNLGYSHPIEFPLPAGISATVEANVITLSGIDNELLGNTAFKIRKLRVPEPYKGKG
                     VKYVDEVLRRKAGKTAAKK"
     gene            complement(17132..17542)
                     /gene="rpsH"
                     /locus_tag="PKK65_00100"
     CDS             complement(17132..17542)
                     /gene="rpsH"
                     /locus_tag="PKK65_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNU81124.1"
                     /translation="MTDSIADMFTRIRNASAIKQAEVLMPMSKIKYEIAKILEREGWI
                     SHAEIVAGGLNARRSQFDQLKIVLKYHKNGNPVISNIKRISKAGSRVYVGKENIPTVL
                     NNFGMAIISTSSGLMTNREAYRQKIGGEVVGEVY"
     gene            complement(17582..17767)
                     /locus_tag="PKK65_00105"
     CDS             complement(17582..17767)
                     /locus_tag="PKK65_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009278448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNU81125.1"
                     /translation="MARTALIVKAKRKPKFSTRKINRCWRCGRNHGYLRDFGLCRICF
                     RELADNGDLPGITKSSW"
     gene            complement(17780..18325)
                     /gene="rplE"
                     /locus_tag="PKK65_00110"
     CDS             complement(17780..18325)
                     /gene="rplE"
                     /locus_tag="PKK65_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNU81126.1"
                     /translation="MRLKEKYQKEIVPSLKEKFSYKNAILVPRLQKVVINMGFGKHAK
                     EKEAISAIESALTKISGQKPILTKAKKSISAFKVREGMVVGAVVTLRGERMYDFVEKL
                     VNISFPRVRDFRGISDRSVDRNGNMTIGLKDYSCFPELKAEDIDQIYGLEICLDTNAK
                     NYEEGLALFQALGFPFKKNKK"
     gene            complement(18336..18662)
                     /gene="rplX"
                     /locus_tag="PKK65_00115"
     CDS             complement(18336..18662)
                     /gene="rplX"
                     /locus_tag="PKK65_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNU81127.1"
                     /translation="MYMNIKKNDTVKILTGKDKGKTGKVLQVLPAEDKVSIEGLNLLI
                     KHMRPSRKGEKGQRIEFPAFIKASNVALICPSCGRQTRVAAKIVAGEKGNKKYRVCKK
                     CQTVIE"
     gene            complement(18681..19055)
                     /gene="rplN"
                     /locus_tag="PKK65_00120"
     CDS             complement(18681..19055)
                     /gene="rplN"
                     /locus_tag="PKK65_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNU81128.1"
                     /translation="MIQVQTKLKVADNSGAKEIMCIRVLGGYRKRYARVGDIIIGTVK
                     SALPHAAVKKSDVVKAVIVRTNKEIRRQDGTYVRFDDNACVIIVDKDKKDPKGTRIFG
                     PVAREVRRAGFVKIASLAPEVL"
     gene            complement(19109..19381)
                     /gene="rpsQ"
                     /locus_tag="PKK65_00125"
     CDS             complement(19109..19381)
                     /gene="rpsQ"
                     /locus_tag="PKK65_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNU81129.1"
                     /translation="MPAQKQTKAISADNKIIKRFHGVVVSAKGDKTAVVKVDTVKMNQ
                     KYQKRYTVSRQYQVHDEENKSKEGDKVTFIECRPLSKNKRWRIIEN"
     gene            complement(19393..19578)
                     /gene="rpmC"
                     /locus_tag="PKK65_00130"
     CDS             complement(19393..19578)
                     /gene="rpmC"
                     /locus_tag="PKK65_00130"
                     /inference="COORDINATES: protein motif:HMM:NF013027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNU81130.1"
                     /translation="MEIKELKTKNPSELQALLAQSREKLRELRFKDSNRQLKNIREIR
                     QIRETIARILTVLNTKA"
     gene            complement(19613..20017)
                     /gene="rplP"
                     /locus_tag="PKK65_00135"
     CDS             complement(19613..20017)
                     /gene="rplP"
                     /locus_tag="PKK65_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNU81131.1"
                     /translation="MLMPKKTKYRKDHKRRRGGVATRLVDVNFGSYGLKAMTAHWINS
                     RQIEAARRVMTRYIKKTGKLWIRIFPDKPVTAHGGEMPMGKGKGAVDHYVCVVKPGTV
                     MFEIEGIDEKTAREAMTLAGHKLPVKCKVLSK"
     gene            complement(20046..20726)
                     /gene="rpsC"
                     /locus_tag="PKK65_00140"
     CDS             complement(20046..20726)
                     /gene="rpsC"
                     /locus_tag="PKK65_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNU81132.1"
                     /translation="MGKKVNPKVMRLGVIKTWPSVWFESGRNYIKNVQQDIAIRKHLI
                     KTMREAGIDKVEIVRSLNKITLNIWTAKPGLIIGRGGNGVEDLKKKIHDQFLRNFRMG
                     EINLNIKEVNRPQLSAQIAVQSAILDIEKRVPFRKVMKQLISRVERGGALGVKVQISG
                     RLNGAEIARTEKLVSGKVPLQTLRADIDYARGVAHTSYGAIGIKVWIYKGEIFEKEKD
                     GKGELIAA"
     gene            complement(20758..21294)
                     /gene="rplV"
                     /locus_tag="PKK65_00145"
     CDS             complement(20758..21294)
                     /gene="rplV"
                     /locus_tag="PKK65_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNU81133.1"
                     /translation="MEIKASLNHLRMSPRKARLVLDVVRGLQVDKALEQLQFLNKLAA
                     APIKKLIESALANAEHNFSLERSNLLIKEIKADSGLTLKRWMPKAHGRATVIRQRGCR
                     VKVVLAEIVESGKKEARKSKVEAPVKLEQIAAEAEKRSKAGAKKDEAVKNTAKTKTES
                     KSGKGFTTKIFNRKAGSK"
     gene            complement(21351..21647)
                     /gene="rpsS"
                     /locus_tag="PKK65_00150"
     CDS             complement(21351..21647)
                     /gene="rpsS"
                     /locus_tag="PKK65_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNU81134.1"
                     /translation="MSRSLKKGPYVNLRILKKVQALKPGDKTIIKVWDRACSIVPEMV
                     GFTIGVHNGKQHVPVYIVENMVGHRLGEFAITRKFVSHGGKMAKAQAQPAKSDK"
     gene            complement(21669..22505)
                     /gene="rplB"
                     /locus_tag="PKK65_00155"
     CDS             complement(21669..22505)
                     /gene="rplB"
                     /locus_tag="PKK65_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNU81135.1"
                     /translation="MSIKKVRANTPGRRGATFDDSADITKHVPEKRLIVRKKKTSGRN
                     SQGKITVRHRGGGEKRYLRLVDFKRDKFDIPAIVTAIEYDPNRGARLALLNYADGEKR
                     YIIAPVNVVVGDKLLSSKNLIEIKNGNAMPVQYIPAGVAINSLELEPGRGALIARGAG
                     NSVFVMGVEGKYAQVKMPSGEIRLVKKECLCTVGQVSNPDKRHIVLGKAGRSRHLGIR
                     PTVRGTAMNPVDHPHGGGEGNQSIGLHHPKTPWGKPALGVKTRRQRSSNKLIIKRRGK
                     NK"
     gene            complement(22531..22800)
                     /gene="rplW"
                     /locus_tag="PKK65_00160"
                     /pseudo
     CDS             complement(22531..22800)
                     /gene="rplW"
                     /locus_tag="PKK65_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005376990.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
     gene            complement(23039..23692)
                     /gene="rplD"
                     /locus_tag="PKK65_00165"
     CDS             complement(23039..23692)
                     /gene="rplD"
                     /locus_tag="PKK65_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNU81136.1"
                     /translation="MSIKVKVYNQKAVPVKDLELDEAVFGLQVNTDLLHQAVVAQQAN
                     ERQVLAHTKDRSEVRGGGKKPWKQKGTGRARVGSSRSPLWRGGGVTFGPTKERNFSKK
                     LNKKMRQKAIMMALSDKIAQSSLVVIDSLKMAEFKTKQFNSMIRAFEKDILPANRRCL
                     LAINGEKDEKAFYSGRNLKGVNIINLENINIVALLTCRQVMINEESLKTLAARYSKN"
     gene            complement(23722..24486)
                     /gene="rplC"
                     /locus_tag="PKK65_00170"
     CDS             complement(23722..24486)
                     /gene="rplC"
                     /locus_tag="PKK65_00170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNU81137.1"
                     /translation="MKFILGKKLPMTQLWDNDRVLAVTPVLAGPCTVTQVKTKALDKY
                     SALQLALGVRKEKNIKKPQLGHFKKAGVAPMHVREFRTENDIDAKIGDQIDVSTFAVG
                     DVVSVTGTSKGRGFQGVVKRHGFQGGRKSHGNKDQLRMPGSVGPKGPAHIFKGMRMGG
                     RMGKERVTIQNLSIAAIDVEKNILYIKGAVPGANDGLLLIKGEGELIFKAVPVVEESK
                     AEEIKVEETPVEESKTGEVLAEAAPVNGIKEEAPAV"
     gene            complement(24696..25034)
                     /gene="rpsJ"
                     /locus_tag="PKK65_00175"
     CDS             complement(24696..25034)
                     /gene="rpsJ"
                     /locus_tag="PKK65_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNU81138.1"
                     /translation="MSEPKKEEKDYKQRIRIKIKAFDHKIIDQATKTIVDTIERSDAS
                     FFGPIPLPTEKRKYTINRSTFVHKDARDQYEMRIHKRIIDIIDPGAKTIEDLTNLNLP
                     AGVDVEIKMQ"
     gene            complement(25132..26319)
                     /gene="tuf"
                     /locus_tag="PKK65_00180"
     CDS             complement(25132..26319)
                     /gene="tuf"
                     /locus_tag="PKK65_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNU81139.1"
                     /translation="MADKFERTKPHVNVGTIGHVDHGKTTLTAAMLAVFGARGMNASK
                     KGVSEIDAAPEERESGITIATAHVEYESKTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHIVLARQVGVPYIIVFLNKCDMVEDKELIDLVEEEVRDLLKK
                     YEFPGDTTPIIRGSALKALENPTGPDAEPIMELMKQLDEYIPLPPRDLDQPFLMPIED
                     IFSIEGRGTVVTGRIERGVIKIGDEVEIVGLMETKKTTIIGVEMFNKSLDRGEAGDNA
                     GLLLRGTKKNEVERGQVLCKPGSVTPHTEFEAQIYVLSKEEGGRHKPFFTGYKPQFYI
                     RTTDVTGEVQLPAGTEMVMPGDTVTITVKLISPVALEEKQRFAIREGGRTVGAGAVVK
                     IIK"
     gene            complement(26416..26745)
                     /locus_tag="PKK65_00185"
     CDS             complement(26416..26745)
                     /locus_tag="PKK65_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81140.1"
                     /translation="MREQLKKALKLARKTNNPIIFFDAETPEDSFAIIDLDRYEKIVD
                     SVTLDKKEAIYKPNLTEEDLADKINREISDWKNQEQSEYLAEESKSRRNWNIPPQIKN
                     KAENHDK"
     gene            complement(26776..28857)
                     /gene="fusA"
                     /locus_tag="PKK65_00190"
     CDS             complement(26776..28857)
                     /gene="fusA"
                     /locus_tag="PKK65_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNU81141.1"
                     /translation="MPRDYALDKIRNIGIMAHIDAGKTTFTERVLFYTGKKHKIGEVH
                     EGSAEMDWMEQEKERGITITSAATTCFWKGNKINIIDTPGHVDFTVEVERSLRVLDGA
                     VAVFDGQAGVEPQSETVWRQADKYHVPRLCFINKMDKMGADFYMSLDSIKQRLNSKAV
                     AIQLPIGAEDNLKGVIDLLACKAYTFTGNFGENITEVEIPEDMKEKVEKYRSDLVERA
                     VECDEVIMEKYLNEEKISVADLKKAIRQGVLNNTIYPVLCGTALQNIGIQLVLDAVNE
                     YLPSPLDVKDVEASDLNDADKKVIVKADDNAPFVGLAFKIATDPFVGKLCFVRVYQGV
                     LQAGSYIINASTGNKERIGRLVRMHANYREEIKEIYAGDIAAVIGLKNTTTGNTLCDE
                     NKPVLLEAITFPEPVIKIAVEPKTKADQEKMGVALSRLAEEDPTFRVETDEETNQILI
                     SGMGELHLDIIVDRMRREFGVEANVGKPQVSYRETITQKAEAEYKYVKQSGGRGQYGH
                     CLLRVEPSGKGQGYQFVDEVKGGVIPKEYIPAIEKGVKEGLLSGVVAGYPMVDVKVTV
                     YYGSYHEVDSSEIAFKMAGLFAFKDACQKAKPILLEPIMNVEVTTPEDYMGNIIGDLN
                     SKRGQIEEMIDRPNKIKAIKAKVPLAEMFGYVTALRSMSQGRANSVMEFSHYSEVPRN
                     ILETIKEKSGK"
     gene            complement(28944..29411)
                     /gene="rpsG"
                     /locus_tag="PKK65_00195"
     CDS             complement(28944..29411)
                     /gene="rpsG"
                     /locus_tag="PKK65_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNU81142.1"
                     /translation="MRGKQAPKRDIEGDGKYNDKNITKLINYVMRDGKKSVAERIVYS
                     SFNLIKEKTKQDPRHVFNKAMKKVSPLVEVRGKRVGGANYQVPFQVRGERRFYLGCHW
                     LITAAHARRGRFMAEKMAAEILDAANGEGAAVKKREAVHKMAEANKAFAHFSR"
     gene            complement(29510..29935)
                     /gene="rpsL"
                     /locus_tag="PKK65_00200"
     CDS             complement(29510..29935)
                     /gene="rpsL"
                     /locus_tag="PKK65_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007474870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNU81143.1"
                     /translation="MPTINQLVRKGRKAKSQRQSSLVLHTSLDSLHRKKNVNVKGAPF
                     KQGVCVKVTTTTPKKPNSALRKIARVRLSNGMEVTAYIPGMGHNLQEHSIVLIKGGKT
                     KDLPGVRYKIVRGVYDAAGVEARRQGRSRYGVKKPKAAK"
     gene            complement(30165..30764)
                     /locus_tag="PKK65_00205"
     CDS             complement(30165..30764)
                     /locus_tag="PKK65_00205"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNU81144.1"
                     /translation="MLALFQIIVLIFSAIIHEYMHGWMADRLGDNTARLAGRLTLNPL
                     VHLEWFGSVLLPGLMLLSGMPFVFGWAKPVPYNPYNLRDRRWGGAKVALAGPAANFIV
                     ALAFGLLLRLLPFISLTFSGLIALIIYINLILMIFNLLPIPPLDGSKILAPFLSPSAQ
                     LKYLNLERFGFIFVILFAWIGFPYLLPLIDWLFRLLAGA"
     gene            30929..31105
                     /locus_tag="PKK65_00210"
     CDS             30929..31105
                     /locus_tag="PKK65_00210"
                     /inference="COORDINATES: protein motif:HMM:NF013026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bL28 family ribosomal protein"
                     /protein_id="HNU81145.1"
                     /translation="MAKRCDLCKRGATKGASRSHSKIQTLKRQGINLQTKNIDGLKLK
                     ICTSCLRTMAKNNK"
     gene            complement(31134..32621)
                     /locus_tag="PKK65_00215"
     CDS             complement(31134..32621)
                     /locus_tag="PKK65_00215"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNU81146.1"
                     /translation="MSASIFLYLFAFFAGLAVASFQAWPPIILIFFAAIGLAFLSRRG
                     RLFFMLLAVFCLGAWRASVSAAADSLLTPYLGKPASNWQFQVCADPEPAWDKQIVILC
                     MLEPSSGKFSKDKIIANLPLYPRVYYGDTLNLHCLLEKPPVFADFDYAAFLAARGIGA
                     LCAWPQVNALEREENRRDIFSRLFIFKREMLRQINQSLPEPAAGLAAALLLGYKKTLF
                     PAENLIWQKAGLSHLVAISGGHITLFLSLLINLLVYLGINKRFALWPALFGAGLYVFL
                     TGWQASAFRSLLMGGIFLYAWRRGRLSSAWLPLLLAGAIMLWNNPNLWRHDLGFQLSF
                     LAIAGMIAFYPLLTSVFTHHFTHSRLRLVFEAFSLSLSAQLTIWPLLAWRSGGVSLIA
                     PLSNILAFSVFSPLMISLISSLGFNFLFGFSEFIWLPSKALLNYLLFLARLAASIPGA
                     YVETPNFHLAYVIAYYFVIILFFLGRYYILTKRRRLILGPTKKDS"
     gene            complement(32666..33763)
                     /gene="prfB"
                     /locus_tag="PKK65_00220"
     CDS             complement(32666..33763)
                     /gene="prfB"
                     /locus_tag="PKK65_00220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNU81147.1"
                     /translation="MDNLGKFLEDLDRRRQELAAVFRIDELKTEIEVLRQQREAPDFW
                     QDQERAVIVNRRAENLQKNYQRWLSLEQSLAETKELAQMATSEKDNKLLAELSDQSLK
                     LSADLAELEKLSLYTGAYDESNALLSVNAGTGGVDAQDWAQMLERMYLRFAERQGWKT
                     EIIDRLLANEAGIKSVMLKITGVRVYGNLRSENGTHRLLRNSPFNADGLRQTSFASVE
                     VIPEILPNDLVIKDEDVRLDVFRSSGPGGQSVNTTDSAVRLVHLPTGITVSCQTERSQ
                     HQNKDNAFSILRARLAKLELEKKEAEEKRLKGGVVKAEWGQQIRSYWFYGNRLVKDHR
                     SNWEDTDVDGVMDGHLDEFVIAYLRWRKDLV"
     gene            complement(33771..35195)
                     /locus_tag="PKK65_00225"
     CDS             complement(33771..35195)
                     /locus_tag="PKK65_00225"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNU81148.1"
                     /translation="MNNDLIQYIQQQIRTTRERIKRYTHSPRGEKYPQRFMFAKIKQY
                     IDGFLDKTQDNRMVIIPGFRGVGKTTLMAQICAAYSRKVENILFLSVEETENLLNAGV
                     NDIIFAFENVIGQSIEELKKPTLIFLDEIQTDPKWAISLKTLFDKTSNVFFCCTGSSA
                     IILQTTTNLVRRALFERMPPMSFIEYEIIKNQTFPKNGLKDKIRQIVYFSNSAEEVFN
                     RLQDLQGDVNNCWSKINRHDIRTYLSYGTLPFSLIMPNETAVYDAISLLLDKIIKQDL
                     PTLGSFDQDTLGAVKRILFAIAENDTTSLGTLEERFKINRLTISNIFEALEKVELLIK
                     VPAYGSNMTVAKKPNKYLFMSPAIRMTFFYFTGQESTYLTRQGKLLEDSFASHLYREF
                     ILKNQGLVRYDSAQGGADFILQIMNNKQIVIEVGMGNKDVKQIATTTKKINSDYNLIF
                     SNTELKINEELKTVFIPLDYYFLM"
     gene            complement(35527..35904)
                     /gene="rpsI"
                     /locus_tag="PKK65_00230"
     CDS             complement(35527..35904)
                     /gene="rpsI"
                     /locus_tag="PKK65_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004826683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNU81149.1"
                     /translation="MQTVGRRKTSVAQVRMYKDGKGVIMINGKKGSQFFVGEGMSIVT
                     QPLKATGHVRDLNFSIIVKGGGITGQIEACRHGLARALLVLDPLLKEALKANKFLTRD
                     PRKKERKKPGFKGARKRPQWSKR"
     gene            complement(36072..36428)
                     /gene="rplM"
                     /locus_tag="PKK65_00235"
     CDS             complement(36072..36428)
                     /gene="rplM"
                     /locus_tag="PKK65_00235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNU81150.1"
                     /translation="MNKIERKIHKLDAAGQAVGRLASQIAIILRGKNKPEFLPYVDGG
                     DIVEVENIAQMSFSGQKLLQKKYFSYSGYLSGLKSKRMSEVFSQNPGEVLRRAVKQML
                     PPTKLRDAQMKRLIIR"
     gene            complement(36448..36798)
                     /gene="rplQ"
                     /locus_tag="PKK65_00240"
     CDS             complement(36448..36798)
                     /gene="rplQ"
                     /locus_tag="PKK65_00240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNU81151.1"
                     /translation="MRHRNTNKILDRKKGPRELMLRNLASSILIYEKVKTTKAKAQAV
                     RPLVERMITAAKAGDLNARRGLIKVLLQKNAVKKSMEVLGQRYQNHPGGYTRITKLGP
                     RPGDGAEMVQIELV"
     gene            complement(36822..37628)
                     /gene="rpoA"
                     /locus_tag="PKK65_00245"
     CDS             complement(36822..37628)
                     /gene="rpoA"
                     /locus_tag="PKK65_00245"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNU81152.1"
                     /translation="MENIALPKKVDFLAGKTENEGVVVVEPLYPGYGMTLGNSLRRVL
                     LSSLPGAAVVGVKIKGASHEFMTLPGIQEDVLEIMLNIKQLHIKMYGDEEVRLELKVK
                     GKQLVTAANITKNSQVEIKNPELLIASLTEEKASLEAEIFVRPGRGYRLSEGTKKENN
                     ELGYMEIDSIFSPVLAVSLDVENTRVGKMTNWDKLLITLKTDGTISPREAFNQAAQIL
                     VDQFSALLPSAQKPAPEIESKEESVDLETKEVAEVDADEADKPAKKKSKK"
     gene            complement(37706..38338)
                     /gene="rpsD"
                     /locus_tag="PKK65_00250"
     CDS             complement(37706..38338)
                     /gene="rpsD"
                     /locus_tag="PKK65_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNU81153.1"
                     /translation="MGRNLDSKCQQCRRVGEKLFLKGERCNTPKCAMVKRNYPPGFHG
                     PSRGRHKLSDYGTQLAEKQKAKKYYNVMEKQLRLTFEKASKKTGDSGKNLLRLLEMRL
                     DNVVFRLGLAKSRSQARQLVNHGHFEVNGVKTDIPSFIVKPGQEIAVRARAKKSPYFK
                     QIAESLKKVELPAWLNLQAAEMKAKVLHEPADTDLPTNFKVQMIIEYYSK"
     gene            complement(38361..38792)
                     /gene="rpsK"
                     /locus_tag="PKK65_00255"
     CDS             complement(38361..38792)
                     /gene="rpsK"
                     /locus_tag="PKK65_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNU81154.1"
                     /translation="MKQKLEKNKQARAKKKLVKKRKKRTARTVKVGRAYINATYNNTM
                     VTLTDSNGDVIAWASAGMAGFKGAKKATPYAAQIITKIAVQKAKEEYGLQEVSVFVAG
                     VGTGREAAIRALNANGLEVSAIKDVTAVPHNGCRPKKPRRV"
     gene            complement(38998..39390)
                     /gene="rpsM"
                     /locus_tag="PKK65_00260"
     CDS             complement(38998..39390)
                     /gene="rpsM"
                     /locus_tag="PKK65_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNU81155.1"
                     /translation="MAIRIAGVNIPNDKRVDIALTYIYGIGNSRAKKILAALNIKPET
                     KVRDLKENDANRLREQIEKKYIVEGDLRREVASNIKRLKEIGCYRGQRHSRNLPVRGQ
                     RTKTNSRTVRGNKRMTATSGRVKAGLKT"
     gene            complement(39437..39550)
                     /gene="rpmJ"
                     /locus_tag="PKK65_00265"
     CDS             complement(39437..39550)
                     /gene="rpmJ"
                     /locus_tag="PKK65_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNU81156.1"
                     /translation="MKVRASVKKICKDCKTVRRKSRVYVICKNPKHKQRQG"
     gene            complement(39595..39810)
                     /gene="infA"
                     /locus_tag="PKK65_00270"
     CDS             complement(39595..39810)
                     /gene="infA"
                     /locus_tag="PKK65_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNU81157.1"
                     /translation="MNAKDFLEMQGEVLELMPAATFRVKLDSGHEILAHLSGKMRMHR
                     IRLLPGDRVHLQMSPYDLTKGRIIFRY"
     gene            complement(39987..41867)
                     /locus_tag="PKK65_00275"
     CDS             complement(39987..41867)
                     /locus_tag="PKK65_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HNU81158.1"
                     /translation="MTANELRQKYLDFFKARGHAIIPSASLLPENDSTLLFTNSGMFP
                     LVPYLLGEKHPAGIRLVNSQKSFRTEDIEEVGDGRHNTFFEMLGNWSLGDYFKAEQLA
                     WWLEFLFVELKLDPSRIYQTVYAGSEDGKIAKDKESILILQDLFKKYGVSAEEGPVTT
                     GKGVLGSGQELDFSRQRIFAYRDKNWWQKGDVVGELGGPDSETFYDTGKEHDLAYGPH
                     CHPNCDCGRFLEIGNSVFMQYQKTAEGWQELKNKNVDFGGGLERILMVLNGQDSIFKT
                     DLFTRAMARIEELSGKKYADNKRAFEVIVDHLKAATFIVGDDHGVPPSNTDQGYIVRR
                     LLRRAIRYGRQIGISQEKWLKEAALIFIDDYAATYPELKNNQEFIFAEFEKEEEKFNR
                     TLEKGMIEFKKLATHDLNGVAAFNLYQSYGFPLEITEELAAEHGIKVDRQGFMAELQK
                     HQELSRTASAGKFKGGLADHSEETTKLHTAAHLLLAALRQVLGNHVSQKGSNITAERL
                     RFDFSYPEKMTPAQIASVEKLVNEAIGKGLDVAFEEISLSEARASGATGVFNSKYGDR
                     VKVYTIGHNNGGRHASWEDIFSREICGGPHVSSLKNLGHFKIQKEEASSSGVRRIKAI
                     LE"
     gene            complement(42047..43069)
                     /gene="murB"
                     /locus_tag="PKK65_00280"
     CDS             complement(42047..43069)
                     /gene="murB"
                     /locus_tag="PKK65_00280"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF000755.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNU81159.1"
                     /translation="MFTLQKQVDISAYTTLKIKAKAAFLAVIKEETELQEALSWARLQ
                     KLPVFILGGGSNILFTQKIKALVLKNEIKGVRVIKETETSVWVEARSGEWWSTLVRFA
                     IERRWGGIENLYYVPGTVGAAPVQNIGAYGVELKDTFVSLRAINLKTGKEKIFTKKDC
                     AFGYRSSVFKNKEKGKYFIASVTLKLSKKPVLRLDYGDIRAQLNEQGIKRPSVQQLAE
                     IIRKIRDNKLPNPAIYPNAGSFFKNPEVTKADFVGLQKKFPEIKFFPGSKSGLVKISA
                     GWLIEQAGFKGKRYGPVGMYEKQALILVNYGGASAKQVLAHVRRVQAGVYQKFGIKIE
                     PEVNII"
     gene            complement(43353..45149)
                     /locus_tag="PKK65_00285"
     CDS             complement(43353..45149)
                     /locus_tag="PKK65_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNU81160.1"
                     /translation="MNENSDKTKEMEPSLSWRLFWRELWRLLSPDHRLINYILAITVL
                     GAVFDLAKPYILKILIDNLYNFQSIDLSFLLWLIVLYLGSGQVRSLIQYFNDRAILKL
                     LVKIEYHLGLKAQDKLLNLSLGYHERENTGAKIVKIERGVDRISTLINNISWEVAPTL
                     VQLAFTIPALFWADWRIGISFLIFTPLFIFVTYWSNQKMYPVRKEIYKSYETASGKMA
                     QSIININAVQSFVQEEREFNEFSDIKAGIRDNENKQWGWMMKIGLGRNIIVDIGRAAV
                     LFLGVYFVYHGEISVGTLIFAFTLSEMAYSSIYRLSRFYDRMEEGRAGVNRLLTLFNA
                     ESEVKNEEGAIIPEKIEGKIEFKDVSFQYREAKVMALDRVSFKISAGTTVALVGPSGG
                     GKTTVARLLYRHYDPTSGKVMVDDKDIKSFDFNAYRRFLAIVPQEVEIFDVSVRDNIA
                     YANPAASLAEIEAAARIANAEEFILKLDKGYDTLVGERGIKLSGGQRQRLGIARAILA
                     RPKILIFDEATSNLDSQSESLIQEALGRIGRHRTVIIIAHRLSTIKKADKIIVLENGK
                     VAEQGSHLELSQVAGGLYAKLLKLQDLGEISE"
     gene            45354..46025
                     /locus_tag="PKK65_00290"
     CDS             45354..46025
                     /locus_tag="PKK65_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81161.1"
                     /translation="METTQITEISVLGKKWKTATSGEKKEISKKISAYLLKADAQQLT
                     TLLPSIKSLSWLTDECLDYVLGKIYDTDKIKKLFPLSRKKTGQRQKLAERYGALIEAK
                     FQSAPFKEKVAIFKEANNQEGFTEIDQENKKFCQRHWQDFIHTINNYEKGLLIYDAFI
                     GNHLTCPEIWATVAAIKTIKVWTKQNNLKKIDSGQAALLQKLNLSLQPGKILVAQEIL
                     KKMGQ"
     gene            complement(46104..47510)
                     /gene="murC"
                     /locus_tag="PKK65_00295"
     CDS             complement(46104..47510)
                     /gene="murC"
                     /locus_tag="PKK65_00295"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNU81162.1"
                     /translation="MDLLKYKKFYLLGIKGVGMTMLAQFLKEQGKAVSGVDIADVFMT
                     DKVLRREKISVRLGFDVSKIPTDAVIIYSSAYTTDNNIEMAYLKNNPDKFKKLPILNY
                     AQAIGALFSSYLGIAVCGSHGKTTTAAWLGFVLQKAGLAPNVLVGAPVPQFKGSAILG
                     KSDYFIAEADEYQNKLQYFQPHGIVLNNIDYDHPDYFKTKAAYARVFADFIKKLPPSG
                     FLTANAADAQIMKLLPLCRAQVFTYALADKVQRRQQNITLLAHSVRRRHGYQYFQVND
                     FGEFKIKLLGEHNVENALAVLATAWALGLDIRKLKTALASFSGAARRAELMGKYGKIP
                     VFDDYGHHPTEVRATLQAFKEAYSGKRLVVVFHPHTFTRTQALFQDFIHSFAEADVLG
                     ILEIYGSAREVQGGVSSRDLVKALKAAGRKKPAVYLSDFDQALSWLKKILKPNDILLL
                     IGAGDIFRVGERLLKKKS"
     gene            complement(47512..48609)
                     /locus_tag="PKK65_00300"
     CDS             complement(47512..48609)
                     /locus_tag="PKK65_00300"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNU81163.1"
                     /translation="MDALKTKLIVISGGGTGGPSTAPLALAVAYRRLDATARFIFLGN
                     SPELEKKLFGRIFEELGADYYALPAGKWRRYFSWSNFFDIFKIISGFFKAYFLFRRLS
                     PDLIVSAGSFASVPVVWAGKLNGAKIFIHQQDLRPGLANRLMSRAADCLSVTFSKSLA
                     DYGQRATLIGNPSLVAEISSNDKERVLVNFKLNNTAPLILVTGGGGGALALNRLFFST
                     VKLLPAGWQIVHQTGPGKGEEAPVRAGYRVVESISHEDYQVLMSLADIVVSRAGLGAL
                     TELSLLAKPCLLIPIPHSHQEDNAAYFADNNAALVLNQETTSAESLANALKSLWQDNE
                     RRRLLSKNISHLLPADAADKGAALMYDLINT"
     gene            complement(48596..49744)
                     /gene="ftsW"
                     /locus_tag="PKK65_00305"
     CDS             complement(48596..49744)
                     /gene="ftsW"
                     /locus_tag="PKK65_00305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HNU81164.1"
                     /translation="MKIGKSLRNFFAPSSQEHQPDRWFLIAAGVLLIIGLVMLSSASS
                     VVSYVKYGNTYHYFFRQLFFVTLGLGAFWGFYKIDYRLWRRYAVWALFISIFLLVLVF
                     IPGLRSEYGTAHSWITIFRQSLQPSEFVKLSFLIYLATWLEAKKGQLNKATSGIIPFL
                     SSLVVISALMMAQPDIGTLFIILFAAFAAFFVAGGKFSHIVLTVVLGAFSLALLLYAG
                     SSYKTDRFKCYQDPSYDSQKECYQINQSLIAVGSGGWLGRGLGQSRQKFMYLPEVWGD
                     SIFSIIAEEVGFVFSTFLIFLYGLLFYRGFKIARYAPDIYGSALATGIMAWLAVQTFL
                     NIGGMINLIPMTGVPLPFISAGGSSILATLMAIGLLANISKHTKYGRS"
     gene            complement(49741..50802)
                     /gene="mraY"
                     /locus_tag="PKK65_00310"
     CDS             complement(49741..50802)
                     /gene="mraY"
                     /locus_tag="PKK65_00310"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNU81165.1"
                     /translation="MTIFYIVKILLLSTLAFIFAVAVTPTLTNFLYKYKLGKKIRNGG
                     QTPVFSKLHAHKAGTPTMGGVLIWGTVLLFMVLFSFLADVMPGEIFKYLNFFSRSETL
                     LPLGVLVASAIIGLVDDWLDVSGRGVFGGGGLKLKHRLLIYTLIAAAGAAWFYFKLDW
                     TVFHIPFFGNLEISWWYIPVFIFIIVATSFSVNETDGLDGLAGGTLMIAFIAYGIISF
                     ALGRYELAAFCGVIIGALLAFLWFNIPPARFYMGDTGAMSLGVTLGVIAMLTNSALLL
                     PFIGFIFLIESLSVIIQTISKRCRHKKVFISSPIHHHYQAIGWPESKVVMRFWVIAAL
                     GAVLGLIIFFVDRQWQFFL"
     gene            complement(50805..51212)
                     /locus_tag="PKK65_00315"
     CDS             complement(50805..51212)
                     /locus_tag="PKK65_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81166.1"
                     /translation="MSLYGRWYNFKSYWGASWKIYWHYRPNRWYLLIGLVLQIILWFL
                     AYRLFIAVGSDLFVSHYNVDFGIDGVGSARRAFLPPILSLAVLVFNFVFTIILSKDQH
                     FHFFAQAAGLSALAAQTLAALALMSLYLINFLA"
     gene            51312..52415
                     /locus_tag="PKK65_00320"
     CDS             51312..52415
                     /locus_tag="PKK65_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNU81167.1"
                     /translation="MSEKIAKTICRYGQANGHSHLRLENKEGKLLCFYGPETGAEYLR
                     LDCSEAASIAETFRYLVGAVDNELFVDKRFKIADKDGVISGRATLLPASNGEKLLITL
                     RNSEPQARRWGALGLNRDQQKILKSALAKKSGVIIIASAAENGSSSTYYSLLQAANNN
                     KSTYSLEAYPGNSLNNTNIINPKKYGGIAASLDKLISLDSELIGVDADLNQNDIKAIW
                     RVAYSGRLIIAVLPALNAAQALKTIKQSGLSTAAIASQLSLILAQKLFPRPCSKCLRV
                     FDPGINIKKTIIQRWPVAATAWPKKLYRNHGCLACRPQANATAVFEIMRFLPDGRLAA
                     GYQPLIIDALTKAGLGLINIEDIAAWADTNKKL"
     gene            52412..53071
                     /locus_tag="PKK65_00325"
     CDS             52412..53071
                     /locus_tag="PKK65_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNU81168.1"
                     /translation="MKIICVIPAYNEDQHLAGVIAKVRPLVDAVVVVDDGSQKKITGL
                     AELAGVFVLRHPINRGQGAALQTGSDYALKLGADIIIHFDGDDQFEAAEIPEVIAPLL
                     SQEADVVFGSRFLEKKSNIPKVKKYIVMPLGRFLNRLWGVRTSDPQSGFRAYTREVAQ
                     SFRIENDRMAHCSEILIKLSQAKWRIKEVPVTVTYHEYGQKLSGGFRIIKDLFLQKIR
                     Q"
     gene            complement(53117..53602)
                     /locus_tag="PKK65_00330"
     CDS             complement(53117..53602)
                     /locus_tag="PKK65_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81169.1"
                     /translation="MKKFFENQIKGLIWFVLAYALVRVFLSNPEMATQGKDLFSIFLA
                     VLAFVWVIFPKIMGEANLPLSISFLSMSGYSLFTWTKFDPGIFAVRWFQDLNDDKIAL
                     LTVLLIFILSAIIFMTDLVSACRETKKRQDILWLVPAWLIFITAIIAAGDYLLPTLIP
                     W"
     gene            53686..54006
                     /locus_tag="PKK65_00335"
     CDS             53686..54006
                     /locus_tag="PKK65_00335"
                     /inference="COORDINATES: protein motif:HMM:NF021575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HNU81170.1"
                     /translation="MLQQAIALAFIAIFIWRLSQQKKKKQIGNNEFILWLAVWLIGAL
                     AIIFIRSLDKILVSLGFSGSGINFLLYLAVISLFYLVFKLRLKISKLEKDLTDLVREI
                     TLTK"
     gene            54019..55197
                     /locus_tag="PKK65_00340"
     CDS             54019..55197
                     /locus_tag="PKK65_00340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNU81171.1"
                     /translation="MKIAQIVCAFPPYAGGIGQSAYRLGEILSREHQVSTFTLVAAKE
                     KNPQKPEARQKIIFLYPHLRAGHGAFPLFLPFRLLSYDLIYLHYPFFGADALILLLSC
                     LRRKQRLIIHYHMDTPALPGFKKVLALPSRLILPRLIKKAEKIIVSSQDYADNGNLAK
                     IAKLYPEKIKAIPFGVETDIFRPKIKTDNSKVVNQAVALVNFVTRRFIKRGGHTLLFI
                     GGLDQAHYFKGLSLLLKSMKLITSTVTLNIIGTGELKNQYEKEVKSLGLSNRVNFLGK
                     VSEDELIRQYQNADLLILPSINEHEAFGLVLIEAMACGVPVIASRLPGVRSVFEDGKQ
                     GYFCEPGNVRDLAEKIDQLCRDENKRQAMGQAARILAKDKYDWEKIADQILDEFSDNR
                     "
     gene            55201..56253
                     /locus_tag="PKK65_00345"
     CDS             55201..56253
                     /locus_tag="PKK65_00345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNU81172.1"
                     /translation="MIIGMANNLFGKYGRGSGAEVIVEKMAADYRQQGHQVFIITTKP
                     KGAADGDRDDCYYLPSNYHRLKDWSTPRKFLWHAGQLLLPGNCRKIKKILQARKPDLF
                     ITHNLLGLGASLPRLLKKYNIRHEHVLHDIQLLHPSGLMYWGQEHIISSPAAKIYQAL
                     TKHLLKNASLIISPSYWLLALHSQHGFFLNQTKKIRRNFNLKKITPSTLKKPINFVFT
                     GQIEKHKGINILLSAWKQANLAPETAKLIIAGDGSWADWLKKEVALINNIEYVGRLDR
                     AGITALLEKSDVVVLPSLVYENSPTSLWEAAAAGKRAIASDIGGIPELSPYLNLLLIP
                     PGDVYALAEAFKTITH"
     gene            complement(56309..57331)
                     /locus_tag="PKK65_00350"
     CDS             complement(56309..57331)
                     /locus_tag="PKK65_00350"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNU81173.1"
                     /translation="MPKQVIFEKKNVLVAGGAGFIGSHLCDELVKTCKVICVDNFSSG
                     SESNIDHLLSNPDFVFINHDISKPLNWSALPELKKFKLEFQGLQEIYNLACPMSPRRF
                     LENRVATLAANSLGVINLLDLALASKAKFMQFSSSVVYGPRQVGAADRKVSENELGLV
                     DQLSSRSCYDEGKRFAETLVVNYHEVFGLDTKIIRLFRVYGPRMELNDDQMIPDFINN
                     ALDGKDLVIFGDDRFSSSFCYVDDAIDAAVKLMGSKEAGPINIGSDVDVNFTDLAMMI
                     IRATNSDSRIVYKDERLFMSELVLPDIRLARENLGWMPVVTLENGLNKTIFALQAQKR
                     LRGFQA"
     gene            complement(57377..59404)
                     /locus_tag="PKK65_00355"
     CDS             complement(57377..59404)
                     /locus_tag="PKK65_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNU81174.1"
                     /translation="MKPFLASFFVFLLLAPLAVSASFLPNDSYYGNQWYLTRIKAPAA
                     WEKINSSPNITVAVIDAGVEIDHPDLRDNIWINRQEIPNNQIDDDRNGFIDDVSGWDF
                     VQNLPDPSPKFDEGWTESGVSHGTMVAGIMAAVGNNYKGISGVTWKAKIMPLRALNDK
                     GEGHIGAVVRAIDYAVHNGADIINLSFVGFTYSQALQDAIQRAYDAGVIVVAAAGNEQ
                     QDGEAYNTDKTPIYPACYDGKNGENMVIGVAATDALDQKTDFSSYGFKCIDLTAPGVS
                     FYSTITHGGNPNDPDKYYDGFWSGTSMAAAVISGSLALIAEANPQLRPAEIVKILLQT
                     TDNISRLNPNYLGQLGSGRVNLERAVTVAQEGLYSHLARLMISPATTTASSSLEIRDK
                     SGVILDAWPLSEAAGGIFAAGDVDGNGEDEIISAAPAGQEPYVSIYGPDGTLRGKFLV
                     YERNFKGGFSLAVADVNDDGLAEIVVGAGAGGGPHVRIFDHKGLLKGQFFAFAKNFRG
                     GVNVAAGNIDGQGAAEIIAVPGQGGAPEVKIFDRRGHLQGQFFAFEKNFKGGLQVAVA
                     NVDGRSDRYKDEIIIAPGFGREPWLKIFSNKGELKKTFMAYGRNWQGGFSVAAGDLDN
                     DGRADIVVGAKPGATPHVRVFSGKGDLLESFYAFPESFSGGVNVGVVNFIN"
     gene            complement(59469..60680)
                     /locus_tag="PKK65_00360"
     CDS             complement(59469..60680)
                     /locus_tag="PKK65_00360"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNU81175.1"
                     /translation="MIIGIDASRANRSLRTGTEWYSFYIIKHLARLDRKNKYILYLDK
                     EVSPDLQAVIAPYPNFQLRRLHWPWRYFWTLGRLSWEMIVRRPDVLFVPAHSLPLFRP
                     RHTVNTIHDIAFLREGGLYQRRGLRLVSKTGKRFLNLLTRLFTRGRYESSPFDYLVWS
                     TAYALKHARTIIAVSEFTRQEILDVYSQKYAEKIKVVHNGYARELYFVRTDKEERRLI
                     LEKYGLEEPFFLYVGRLEKKKNTQALVEAFALFKETNPHSDISLVLIGNAGFGYDEVK
                     YIIEEYNLSRQVLMPGWVAEEDLPIIFSAALAFVFPTLYEGFGIPVLQAMACGVPTLV
                     SDIPVLREILGEAPIYFNPRDKNELAASLATVAGNENLRRDLISRGLARANQFSWEQS
                     ARETLKILTER"
     gene            complement(60691..61698)
                     /locus_tag="PKK65_00365"
     CDS             complement(60691..61698)
                     /locus_tag="PKK65_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNU81176.1"
                     /translation="MFIKRIGIDLGTTYTLVYLPRRSIVINEPSVVAISLDDNKILAV
                     GNEAKDMLGRTPDTIVAARPLKDGVIADYRTTEAMIRYFINKTLGGVRLFRPEVMVAV
                     PAGISSTERRAVIEATIAAGAKAAYIIKEPVAAAIGADIPIGSATGHMIIDIGGGTAE
                     MAVISLGGIVASTSVRVGGNKFDAAILEHIRKKYNLAIGDRTAEEIKINIGSALYIED
                     KLYMEIRGRDIVTGLPRSITVSSDDVTEAIQNELEAIIAAAKRVLQKTPPELAADIMD
                     KGIILSGGSALLRNIDQLVARTTGVPAYIADDALLCVAKGTGIALDNLDSYKRSILAT
                     K"
     gene            complement(61797..62225)
                     /locus_tag="PKK65_00370"
     CDS             complement(61797..62225)
                     /locus_tag="PKK65_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNU81177.1"
                     /translation="MANKKIFILEDDVNLLYGLEAQFSADEFVVESSEGEEELEELLD
                     NIRSFSPDYMILDLILPKLDGFEIIKRIKEDEELSNLSIFIFTDLSDEDSKNRSLELG
                     ADYYFIKDDFDTYEFAEKVKKIIANQTENENSEVADTYEE"
     gene            complement(62268..63248)
                     /gene="rfbB"
                     /locus_tag="PKK65_00375"
     CDS             complement(62268..63248)
                     /gene="rfbB"
                     /locus_tag="PKK65_00375"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNU81178.1"
                     /translation="MKLLVTGGAGFIGSNFIHYWLKNHPDDRIINLDVLSYAGNLENL
                     RDLDNNYAYRFVRGDITDIDLVDSLVAEVDAIVHFAAESHVDRSILNSADFVRTNVEG
                     TRVLLDAAQKHGGVRFHHISTDEVFGTLPLDGGRFNENTPYNPRSPYSASKAASDHLV
                     RAYFYTHALPITISNCSNNYGPYQYPEKLIPLFITNLIDGKKLPVYGRGSNVRDWIHV
                     DDHNRGIDLILQKGKIGETYCLGGNSERSNLEITKKLLSIFGQGEEMINYVADRPGHD
                     LRYAVDFSKAKRDLGWEPLIVFERGLAETVDWYRQHEDWWRPIINAKTKN"
     gene            complement(63245..63646)
                     /locus_tag="PKK65_00380"
     CDS             complement(63245..63646)
                     /locus_tag="PKK65_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018124322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNU81179.1"
                     /translation="MCLFCKIIAGEIPSFKVYEDGQVLAFLDIAPVHKGHLLVVPKRH
                     YANLEEIPDEELQSLITVVKKCGLMLKTKLAYAGYNVFENNDPVAGQEIPHLHFHVIP
                     RIAGDNLPLWRKEAYAPGEAEAICSRLKAVL"
     gene            complement(63651..64103)
                     /locus_tag="PKK65_00385"
     CDS             complement(63651..64103)
                     /locus_tag="PKK65_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HNU81180.1"
                     /translation="MIKGVIIKKIKKFSDDRGWLMEIYRRDESDLAPAMSYVSLTLPG
                     VIRGPHEHVYQSDFFIFIGPGRFRLHLWDRRADSSTKGEKMEIEVGEDNPCSVLVPPG
                     VVHGYQCLSGVPALSINLPDKLYKGVLKQDEIDEIRWEQNPDSPYKIN"
     gene            complement(64130..65113)
                     /locus_tag="PKK65_00390"
     CDS             complement(64130..65113)
                     /locus_tag="PKK65_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNU81181.1"
                     /translation="MKNHIITVGGATRDTTFFTDEGVLIDNQRDVLRQKLLAFEYGAK
                     IKIDRFYFLFGGGAANAAVNFAVSGFKTACLARVGQDESGRAIIANLRQHGVITSLVS
                     SEKNRETMISFILVTPGGERIIFTSRAAHNDLRISRQEERILKQADWLYLASLPGDWL
                     PAARKIFSLSGPKIAWNPGASQYQAGLKKIASFLKRTSVFCVNKDEAIELVLSDARYR
                     GQSPAFLNNVKNLLVALKAAGPEIVLITSGQDGADVYDGHNFYHQAVFPEKKRVDITG
                     VGDVFNSSFVAGWELSGGDIKRALYLGAKNTASKIAYFGAQNGLLDLRQIK"
     gene            complement(65145..65684)
                     /locus_tag="PKK65_00395"
     CDS             complement(65145..65684)
                     /locus_tag="PKK65_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81182.1"
                     /translation="MTRQIFTYFALLILAGFLAIIQFSLVSAWSFPWNSVNLIIVAII
                     SAFLIFNKDQVWFLALAAGWFLDVLGFHPFGVALLSLLFSTAVVYLVLENWLTNHSLY
                     AFLLLTVIAVLVEALSYNLLLLIFARSGSAEKLFLFSDYFWKDLAATAVISLFLVGLF
                     FNLLALAGRRLKPFFLKRP"
     gene            complement(65687..66499)
                     /gene="mreC"
                     /locus_tag="PKK65_00400"
     CDS             complement(65687..66499)
                     /gene="mreC"
                     /locus_tag="PKK65_00400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNU81183.1"
                     /translation="MRSFGKSRKIWYWLASLALIFILHFSGLTRPLENYLFLAIKPFA
                     AALQRLGERSRGRPESATDVSTLLEQLDKAKADLAAAIVDQAKLQLLEEENNKLRKQL
                     DFVNANNYQVLSANIVSRQNLFESAEDAQDIVLDKGAAEGLRPGLGVIDESGVIIGKI
                     VEVKDHSARACLTTSTNCQLPAAIINNTKTIGLAEGELGLTIKMNYIPQLEEINQGDI
                     IITSGLGGDIPRGLVIGRVAQVNKQSNEIWQDVTIEPLSSWHSLTIVSVILP"
     gene            complement(66507..67553)
                     /locus_tag="PKK65_00405"
     CDS             complement(66507..67553)
                     /locus_tag="PKK65_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNU81184.1"
                     /translation="MFNKLVGKFSHDIGIDLGTKNTLVVMSDKGLIINEPSVVAINKR
                     TNEILEVGEAAKKMVGKTPAHIEAVKPLVDGVISDFEVTEKMLKYFINKVHREHFALF
                     PRPRVVIGIPLDLTEVEKKAVEDAVKSAGARKVYLVEEAMASAVGAHLPVTEATATMV
                     VDIGGGSTEIAVISLGGVVTWKSLRLAGNEFDNNIIEYIREEFNILIGEQLAEEIKIK
                     IGSAIAGEESQEMEVRGRDLINGLPKAIIVNDKQIREAIGRTVSKIIENIKTILETTP
                     PELVSDIYERGVYLSGGGALLRGLNQAISLATKIPVKIVDDPLTCVVRGAGILISDSE
                     LLAKVTLGNVSLDK"
     gene            complement(67543..68769)
                     /locus_tag="PKK65_00410"
     CDS             complement(67543..68769)
                     /locus_tag="PKK65_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HNU81185.1"
                     /translation="MKQSQLFTHTLKELPKDETSYNAQVLLRAGFIDKVAAGVYSFLP
                     LGWRVMEKIRQIIREEMQSIGGQEINLPTLAPKENWQKTGRWESLDILFKIAASDKKE
                     YALNPTHEEVVTPLAGKFINSYRELPFAVFQIQTKFRNEKRAKAGLLRGREFTMKDLY
                     SFHADQVDLDRYYEQVIQAYVRIFNRVGLGDNTYLTFAAGGSFSKYSHEFQTLTAAGE
                     DLIYICDKCHLAVNQEIIAEQSVCPQCGDKDWRKEKAVEVGNIFKLGTRYSDAFALSY
                     SDKNGVKQPVVMGCYGLGLSRIMGTVVEVCHDERGIIWPQSIAPFRVHLLSLKANEEA
                     EKIYQKLGQDGIEVLYDDRDVSAGEKFAEADLIGCPYRLVISQKTLIQNGVEIKRRVS
                     QETEIIKFNHITAYVQ"
     gene            complement(68777..69364)
                     /gene="ruvA"
                     /locus_tag="PKK65_00415"
     CDS             complement(68777..69364)
                     /gene="ruvA"
                     /locus_tag="PKK65_00415"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNU81186.1"
                     /translation="MFAYLRGRILAKSNNYLILETNNVGYQIFVGEAFWSSLKVGEEQ
                     EFYLSHQVRADASDLYGFRNLAELELFQLLLSVSGVGPKSALGVLSVASIGDIREAVI
                     RGDAALLTKVAGIGKKTAERVVLELKNKIVKSIDGASLGSAFSSGFNDELDALLALGY
                     SLSDARNALGELDVDLKDSGERVKAALKKLSRKNQ"
     gene            complement(69519..70430)
                     /locus_tag="PKK65_00420"
     CDS             complement(69519..70430)
                     /locus_tag="PKK65_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNU81187.1"
                     /translation="MSNLYNYSAVNQRKTWFLMTGFFIFVILIGYIFSQAMDAPIILP
                     AAVIFSILMSFFSYWYSDKIVLSMSGAKEVNKNNGRELYRLVENLCITAGLPVPRIYI
                     IKDSAPNAFATGRDPQHAAIAVTSGILGKLEKAELEGVIAHELSHIGNRDILISTMAT
                     VLVGIVVLLADWFRRWSFFGGRRHNDNNNNNQLGVIMLILALVFSILAPIFAQLLQLV
                     VSRKREFVADADAALLTRYPEGLARALEKISADREPLKVASRATAHMYIVSPFKGQEK
                     KNQVSWFAKIWMTHPPVAERIAKLREV"
     gene            complement(70443..71201)
                     /locus_tag="PKK65_00425"
     CDS             complement(70443..71201)
                     /locus_tag="PKK65_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HNU81188.1"
                     /translation="MWIYLIIFIGVLLLAFLKQVNQYERGVVFTMGRYSYVMQPGWRL
                     LIPVFQTFHKVDIRVKAVDVPDQKAITRDNVPVTVNAVIYYKVTAAEKAIIEVENFKY
                     AISQYAQTTMRNIVGSVTLDELLSKRDEIADRIKEIVDKETDEWGIKVNNVELKDVSL
                     PESMERTIAKQAEAEREKRAVIINSEGELAASANIAQAARTLSEVPGALHLRTLQSIN
                     DLSSDQSNTVIFMTPLEVLKAFEGFAQKMNNENK"
     gene            complement(71259..71816)
                     /locus_tag="PKK65_00430"
     CDS             complement(71259..71816)
                     /locus_tag="PKK65_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNU81189.1"
                     /translation="MTTWIILGIIVLLALVLIAMYNGLIRSKNRVDEAWSDVDVQLKR
                     RHDLIFNLVETVKGYATHEQSTLDNVIKARNTAVQAQDSRDPKAVLEAENALSSTLKS
                     IFALAESYPDLKANQNFLELQRDLTDTEDKIMASRRFYNANVRDFNTKLQVFPTNILA
                     KQLGFKAYNFFEAVEAEKEAVAVKF"
     gene            complement(71909..74824)
                     /gene="ileS"
                     /locus_tag="PKK65_00435"
     CDS             complement(71909..74824)
                     /gene="ileS"
                     /locus_tag="PKK65_00435"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNU81190.1"
                     /translation="MSNEQNQNKTVTPNFSEAERSILSFWQDGGIFDKSLVKDSPKGD
                     YVFYDGPPFATGTPHYGHIVASVMKDVVPRYQTMRGYHVERKWGWDCHGLPIENIVEK
                     ELGTKNKKDIEELGVGKFNDMCRAKVMTYAKEWERVISRLGRWVDFDNAYKTMDLNYM
                     ESVWWTFKQLWDKGLIYKDYRSMYICPRCETTLSQSEVTEGYKDIKDLSAIAKFELVD
                     EPGTFVLAWTTTPWTLIGNVALAVGKEIEYVKVKFDGANYILAKGRLEEVFKNKNYEV
                     LDACKGSDLIGQAYKPLFNYYSEDKNLENRDNGWKIYAADFVTTEDGTGVVHIAPAFG
                     EDDMNLGKQESLPFIQHVGLDGMFKKEVADFPGLHVKPREDHLATDVEIIKYLAAHNL
                     LFSKEKYEHSYPHCWRCDTPLINYAASSWFVGVTKIKDKLRETAKEIKWSPDYIKNGR
                     FGNWLEGARDWSISRQRFWASAIPIWECPAGHRHVVSSVAELEERTGKHIDDIHKDKV
                     DPLVFACPECGAPMRRITDVIDCWFESGSMPYAQLHYPFENKEKLEKNFPAQFIAEGA
                     DQTRAWFYYLHVIAGGIKETEAFKHVIVNGIVLAEDGKKMSKKLKNYPDPSLVMEKYG
                     ADALRAYLLSSPVMQAENLNFTESGVQESLRKNVMTLWNVYRFYEIYAGEYDGREVTA
                     ENILDAWILAKLKVLNIEVTAAMEAYNLPKAMRPITEFIDELSTWYLRRSRERFKSEV
                     ESDKQAVLAMTRFVLLTLAKIMAPFMPFIAERLWQEVSGYKLEKRDESVHLQSWPNLG
                     QLTEEEEAVLANMQLARKGVELALSARDAAGIKIRQMLNSAEFTGGGKLAEQYFVLLS
                     EELNVRSVSWQGGGGSLEVKLDTVITLELQREGNKREIIRTINSLRKEAGLTVADRIE
                     ILIESSEESFSWLAGWSEELKKATLADVIRQGREDNPLAVKVLKLEQIDLSLQIVKL"
     gene            complement(74920..75984)
                     /locus_tag="PKK65_00440"
     CDS             complement(74920..75984)
                     /locus_tag="PKK65_00440"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNU81191.1"
                     /translation="MTNNRLSKRIKLTQNILAKIAKIDQFQGLWQGSLRLSPQILGRL
                     KTWVIITSTGASTRIEGVKMTDEEIARFLRGWKGKRPKSRDEQEVAGYADLIGRIFDN
                     WKTIKLTEGWILQLHGILLQFSEKDQTHKGKYKDSPNTVVMTNRQGEPVVLFDPTPPY
                     LVKAEMQEAIAWTNEQLEKKEIHPLLIIANFIFEFLAIHPFKDGNGRISRALTNLLLL
                     KAGYSYTPYVSLDEIIEQTKAEYYLALRATQKNHKTKHEDITPWVEYLLSSLLEQSEK
                     ARKLMESEQPEKLLSEKQTQIYQLFEKNDELSVSEIDKLLKGKIPQATIKQALSRLVT
                     WKLLERIGQARSTRYKKPNH"
     gene            complement(76165..78906)
                     /gene="secA"
                     /locus_tag="PKK65_00445"
     CDS             complement(76165..78906)
                     /gene="secA"
                     /locus_tag="PKK65_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNU81192.1"
                     /translation="MDLLNKIFGDPNEKIIKELRKTVEKINALEPSFQALSNEELLNK
                     SLEFKDRLKNGISLDEILPEAFANMRETSKRILGQRHYDVQLIGGIVLHRGQIAEMKT
                     GEGKTLVATLPLYLNALSGEGVHLITVNDYLARVGAGWMAPLYHALGLSVSVIVHEAA
                     FKYDPEYNDESQYDERLRHFRPISRREAYYCDITYGTNNEFGFDYLRDNMAYTLSQTV
                     QRSLHYAIIDEIDSILIDEARTPLIISAPAEESTDKYVKFAQLVQGLTENEDYNIDEK
                     MKAATLTEAGINKIEEWLGVGNIYVSGGVKDVHHLEQALKAMVLFKRDRDYVVKDGEI
                     IIVDEFTGRLMYGRRYSEGLHQAIEAKEGVKVQKESQTMATITFQNLFRMYNKLSGMT
                     GTALTEAEEFYKIYKLETVVIPTNKPNIRKDLNDLIYRTEENKFNAVIREIKARHEKG
                     QPVLIGTISIEKNELLANMMAREGLHPQMLNAKNHQKEAEIIAQAGRVGAITLATNMA
                     GRGVDIILGGNPDTNDEAALAEAQAEQEKVKALGGLHVIGTERHEARRIDNQLRGRAG
                     RQGDPGSSQFYVSTEDDLMRIFGGDRMKAMMKTLKLPPDVPIENRMISKSLESAQKKV
                     EGNNFDLRKHLVEYDDVINKHREAIYRRRREILEASEMEVKEGENTPLIKLNALINEQ
                     VKEEITKVIVFHTSGDKSGDWNFKEIVETLRTMLNVSEQEDKLKELAGDGGEAGRQKV
                     LDYVQELAAATYERLVSDFTQAGIDFVEVAKGLLLRSIDQMWIDHLETMDYMRRGIGL
                     RGYGQRDPLVEYKKEAYRLYHELLDLIRQQLVYSIYRTGDILKMAPSALIRLSGSEQP
                     KNLKLEGAAKEMEKLSAKRDNFDLVRDKVKDEFGKTVGRNDPCPCGSGKKYKKCHGR"
     gene            complement(79071..80750)
                     /locus_tag="PKK65_00450"
     CDS             complement(79071..80750)
                     /locus_tag="PKK65_00450"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HNU81193.1"
                     /translation="MQWLNEYSRSFLAKGYLNPGISAEQRIKDIAKTAEKILNKPGFA
                     DKFYDYMSRGYYSLASPVWSNFGLSKGFPISCFSSYLGDDMGQILFTQAEVGMMSKYG
                     GGTAGYFGDLRHRGAAIKDNGHSSGAVHFMQLFETLVNVVSQGSVRRGHFAPYLPLEH
                     PDADEFLNIGTEGNPIQEMTYGVTVTDAWMKAMAAGDADKRALWAKVLQRRSQMGYPY
                     IFFTDTVNNNTVDVYKDKGLKIKASNLCSEILLPSNEEWSFVCDLSSLNLLYYDEWKD
                     TDAVETLVYFLDAVMSDFISRLEALRDSDDARKRQSFLFMERAYNFAVANRALGLGVL
                     GWHSLLQSKLLPWEGKESARLNVAIFKDIREQAIAASKKMALEYGEPSILKGYGRRNT
                     TLMAIAPTTSSAFILGQVSQSIEPIWSNCYVKDIDKMKVTIKNQQLEKLLEEKGQNSK
                     EVWHSIRDNDGSVQSLKFLSPEEKEIFKTFQEINQKIVIEQAAARQTYIDQGQSLNLA
                     INPAVPVKEVNALYMYAWQLGIKTLYYQHSVNAAQQFGRLKDLQNCQSCQA"
     gene            complement(80795..82105)
                     /locus_tag="PKK65_00455"
     CDS             complement(80795..82105)
                     /locus_tag="PKK65_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HNU81194.1"
                     /translation="MDKINVSQRKYNFSVQKRDGKIVAFDQAKITATIFKALAAVGQG
                     SAADATALAETVARQAQIEAESSGASGSFRLNVEKIQDWAEQALRESSFKEAAQAFIL
                     YRDKRAARRRRDIFKKRTNLKPYEYPELYEYVRAIRHSYWIHTEFNYLSDIQDFKTRV
                     TPVERQAIQRSMLAIAQIEVAVKVFWGDIYKKMPKPEIAAVGFTFAESEVRHMDAYSH
                     LLEILGLNEEFARIKTMPVLMERVEYLEYSISNAKREDNKDYTLAILLFSLFIEHVSL
                     FSQFLIIMSFNKHKNLFKGISNAVEATSKEEQIHGMFGLEVINLIKSEHPEWFDESFK
                     EKIIQAGYVAFDSEKKVLDWILEAGELEFLPKTVIIEFIKNRLNNSLDSIGLERIFPV
                     DEKLLEQTEWFDEEIVTTKHGDFFVKRSINYNKRSQSITGDDLF"
     gene            complement(82277..82600)
                     /gene="raiA"
                     /locus_tag="PKK65_00460"
     CDS             complement(82277..82600)
                     /gene="raiA"
                     /locus_tag="PKK65_00460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNU81195.1"
                     /translation="MKINLKTTHLELTEAIRHYAQSKMDMLEKYLGNIQVINCDLELE
                     KSVGGQNKGEIFRAEINLQIPREILRVEKTEADLYKAIDKVKDHLEEIIISYREKRRD
                     QKRGK"
     gene            complement(82625..83767)
                     /gene="rseP"
                     /locus_tag="PKK65_00465"
     CDS             complement(82625..83767)
                     /gene="rseP"
                     /locus_tag="PKK65_00465"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNU81196.1"
                     /translation="MMTVLIFILVLSVLVFVHELGHFWTARKLGVKAEEFGFGFPPRL
                     FGFYRDQQGQRRHFWGNRSLDELPLEQKPKGTVYSLNWLPIGGFVKIKGENGEGKQEP
                     DSFAAQSLGKRALILAAGVIMNIVLAFFLLSLGYMLGLPQSLDEAGPSARITQAQVWV
                     METLPQSPAADAGLEAGDVIMAVNGQAVSSSENLQALVAENNGVATNFLVKSGDEEKQ
                     VTITPTVIASTGKPGIGVSIYASGKVRYPFLIAIWEGFKATGLMFWAIIVAFATLIRD
                     LFIGASVSGQVTGPVGIATLTGQVASLGFTYLLQFMALLSLNLALINILPFPALDGGR
                     ILFVVIEKFKGKPVKQSVEAWIHTIGFWLLLALIVVVTYKDIGRLF"
     gene            complement(83777..84331)
                     /gene="frr"
                     /locus_tag="PKK65_00470"
     CDS             complement(83777..84331)
                     /gene="frr"
                     /locus_tag="PKK65_00470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNU81197.1"
                     /translation="MNNFLSAKQAEFEAVSDFFKRDIATLRTGRANPAVLDGLQVEAY
                     GVMNPLNAVANIAVADARSITVSPWDKGVSKAVEKAIIEADLGFGVVNEGDKIRLSVP
                     SLTEENRRDIVKKLNEKMEKTRISLRQLREEVKTKIEQAEADKEISEDEKFRAMKELD
                     EFVAKKNEEIKEARDRREKDIMEI"
     gene            complement(84345..85457)
                     /locus_tag="PKK65_00475"
     CDS             complement(84345..85457)
                     /locus_tag="PKK65_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81198.1"
                     /translation="MRYLKIFILLIAVTVLTSACTLSLGPNGISTSSNTDGGVWLSVD
                     KGKTWKQMALVPTITGQPYSLGGVDTSVLTADPQDSNAVYFGTVGKGLYYTYNINNGW
                     NEVKSLGQGTINDVKVDPKSKCTLYAAITNRLYRSNDCGRSWQQTYYDNNPGVGVTTI
                     AVDYYNNNNIYIGTSRGDIIKSIDQGSSWRTIQRLNESIARLIVSPQDSRLIFVATAK
                     NNIYSFNSNTVTDATDSAAIDRNFAVENWTDLGVVLKDFNLGSNFRDIVTCAADGSLF
                     LASEKAIVRSPDKGMTWENIKLITPEKDAIINAIAVNPQNSNEIYYVTNTTFFSSSDG
                     GVTWTTKKLSTSRAGWDLLIDRSNPNIIYLGTKKLQ"
     gene            complement(85462..86442)
                     /locus_tag="PKK65_00480"
     CDS             complement(85462..86442)
                     /locus_tag="PKK65_00480"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HNU81199.1"
                     /translation="MADIKNRFDWPPIGQDKVVAFLEKSLVSGKLAQTYIFAGPKDLG
                     KSSLALAFARNLWQSDRGAAGSGNFESLNSDLYILEKDDDKQVIGVEATRNFIKRLGL
                     SSFMNSYKIGIIKEAETLSSESQSALLKTLEEPRAQVVIILLTEKLEALLATIVSRSQ
                     VLYFQPVKSAAVYDYLLASLDIKRSKAKELAAASLGRPLQALRWAENQDSYQDYFQLV
                     GQLFTFLKSDLAFRLGFINQESGGDKLSAEEASRWLDIWESLWRDALLVSLGQEEYLR
                     YPSLLDSWKKDFNLSAEVVIDRLRQTTKARLYLQGLVSPQNILESLAIYF"
     gene            complement(86447..89254)
                     /locus_tag="PKK65_00485"
     CDS             complement(86447..89254)
                     /locus_tag="PKK65_00485"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81200.1"
                     /translation="MNFLAKSYFLASVAALAFLFLGVSPLPASAAVCNADTLLDVVAR
                     DPGGSYISGIKAELYYQVIDANGKPKPGAKVASASANASTGIATLKFRNSNAESDVYA
                     LKIQAINKDFTSYWYYDLKLSCGQQISLEKILSGINFVLRDYNGNLLYNTNFNVYTQN
                     YDLDGNPIKQTKDLVATLNTGTSGGARIYVPQGSVRSLDGSRSDDYVLELTRNGHKFD
                     LYGIKVKDELLTNSEYYSSAVKVTLRTATGALFPGKTKVEVYEQGIDGNNDEVQGDKV
                     GEFQTNDDGYGIFEYPAGVYVLGVKGSNGQYDYLWDVEILDGQLNEYDWQVGTSWQSG
                     KETCADNVKLTINLMGMGGEALSGFKYEVYEQEVDVFARPIAGKKIGGGTTNSAGKAE
                     LTFKPDPRKSYALKAYDKKSDLGEFWFFDAARFVCGYDRTITKSLPYLKVVLRDARGN
                     LKKDFSFSLYEESFDVDNRPVKDAKKLIATLKTGSNGVATIYVAPAHPFDQNKRGLYI
                     FSATVNKSVFDVYNIAVEANKNTTFEYVFSDLSLSVKSASGQAAANTEVKLYAQTVNG
                     STYTLGDLLTSGKTDSSGNLHLEYPAGTYALAVTDSFKRNNIFWNAVIKDQQANRFNL
                     SLNVTRVTLASGLGELMPANTVLKVYSLYESGGYFYKDKEVGSIKLANNKQGEALLAD
                     GPYLLSYIDKNKIEYGQAFWAQNGKINKLNLKMEKAQQIEAGQKFTLSRPALSVAISA
                     STPNSSSSATASGSLVKRLAGYILLQVEDKGQAWYLNPKDAKRYYLANGAAAYQIMRQ
                     SGVGITDADLRKIPIGVDSRFSGNDSDGDLLPDGLEVAIGSNPNNSDSDGDGYLDGEE
                     LRSGYNPNGPGSLSYDLSFAAKQKGKILLQVQKNGEAWYVNPQDGKRYYLGNGDLAFQ
                     VMRYLSLGISNQDLNQISIGQ"
     gene            complement(89386..90735)
                     /locus_tag="PKK65_00490"
     CDS             complement(89386..90735)
                     /locus_tag="PKK65_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81201.1"
                     /translation="MLKFNKKTYIYQFSGLLLIIFSIFFFNISQVKALSPEINQVKVP
                     GESTVYYLDHATHQRKAYINEAVFLDYGNDWSDIKIISPEELAKWPEARLIKTAESDV
                     LYYIRGSEKVKINTLQDIMVYNLENVLPITVSDFELSQYASEDSYEAAGLAKNSGLSV
                     YQALLDNPQSGNHLVAGTHDNQVMLLYFTAGDEAALFQNITFKLSGIYSDSLIDSVSL
                     VNADTGKKIEGSSSYHDRQLTFRFRQNSFYVPAGNTIKLKVLLDLNYLDNVNNQTIRL
                     QLENPQAVTSNLALSGSFPLIGTEFKMVDAGHILGTVVAEEQPLGANGSVKNIGKFRI
                     SETSGNEDVYIRQLVFRNNGSASSNDLELFKLRRGNQLVATARSMEDGQIVFDVNYLR
                     LLAGDSLELSVSANLNSDYQSGRSVDLELNSLTAVGTNYNLSLAPSIRNINESFLLP"
BASE COUNT        27027 a        21697 c        17050 g        25027 t
ORIGIN      
        1 ttataactcc tttattatcg cttgattttt catttatctt aaatttatct aaattataca
       61 actttatgaa aacgggcgca agggtttcga atcagggttt cgaatcaggg tttcgttttt
      121 tggcagggtt tttggctcta taataattag tgtggtggat aaaacaaaaa tagtggtaaa
      181 attagctgta aaataaagtt tcccaacttt agctaataat taaccactat ttctatgtta
      241 aaatgcatca acaaaatgtt aaacctgcaa gggtttttaa ttggcaaaat caaaaatagg
      301 cttatatcag cctattttat tcctagctcc cggggtggga ttcgaaccca cgaccaattg
      361 gttacactta cccactaatt tcttaatggt gtggactata tcatcatcca aataatggat
      421 gcgaggcact tcgggccgtt ctaagaacgg gcctactcct tttcaggata gtctctgaac
      481 cttccctaac gaattagggc ttggctgctg attaccataa tctttcgatt ttaggcttcc
      541 agacagttca cctcgttttc tttccacaat tacttgagga agctgcgtgt tgcttacgtg
      601 cacctcacga tgacaatttg cgcctaacaa tatacattta tccagctctt tctttataac
      661 ctgctagctt ctggtataac ctttagcaga tataccaaaa ttttttatca gattccaagt
      721 gatgaaattc aagagccttt aaacatctat cgtcaccgca aacggaacat ttgccacctt
      781 tatattcaaa ggctaattaa cggatttgct ttcgccacca ttaaccatcg cctttaaata
      841 ttcggcacga tcagcgtctt ttctcttatc ttttgtcata ataatattat aacaaaagag
      901 aaagtgtgtt cacagccaac tgcgctaccg ctgcgctacc cgggaatatt aaattgtcga
      961 aagtcctgct ccctgcttaa aaaacaaaat atttcaatag aaatatttac taatataata
     1021 ccagattagg acattatgtc aataatacta aaattaaaaa acttctcttt aaaattaata
     1081 atccttcagc ttctccaagc ccttcagttt tctgattttt tccaaagcct tactttcaat
     1141 ttgacgaatg cgctctctgg ttacttcaaa ttcttggccc acttcttcca aagtatgagc
     1201 tacgccgtca atcaaaccga agcgcatctc taaaatcttc tgttcacgcg gagacagctg
     1261 agccacgata ttcttcacgt aatcacgtag tagttgtaag gcggccgccc tgtccggagt
     1321 gatattcttc acgtcttcaa taaaatcctc tagggttgaa tcctcctcat catcgccgat
     1381 gctggtttcc aaagaaatgg tgtcttggga aatcttgata atatatctca ccttttcaat
     1441 attttctccc atctctgaag cgatttcttc agccaaaggc tcccgcccca attcctggat
     1501 taaagttctc tgcacctgct ggaatttatt aattgtctcc accatatgaa ccggaatacg
     1561 aatagtgcgg ctttgatcag ctaaagctct ggtaatagcc tgcctaatcc accacgtggc
     1621 ataggtagaa aacttatagc ccttacggta ttcaaacttc tccacggctc taaataggcc
     1681 aatattgccc tcctgaatca agtccaaaag acttaagccc ttggccccgg caaacttctt
     1741 ggcaattgat accactaaac gcaaattggc ttcaataatc ttcttttccg cttctttatc
     1801 tcctttttct ttcctcttgg ccagttctac ttcttcctca ccgctaagca aagggacttt
     1861 acctatttcc tttaaataca tctgaatgga atcggcgctc aatttagaca aatcaaaagt
     1921 aacagccgcc gccgctttgg tcgaagtaat gtggggattc ttttcctgca catccaaaat
     1981 ccggccagaa tcttcagcga tacggatgcc attcttctgc aattctccta aaaagacatc
     2041 atattcataa atatattctt ccacttccgg aaaaagatac aacagctccg tttctgtaac
     2101 gaaaccgcgc agacgccctt tattaagaaa cttctttaat tgctcttcgg tcggtcggac
     2161 aacttcacgg ggcgtaagcg ccactttggg cttattttga ggtgtcgtct ttatcgctgg
     2221 cgccgccacc ttctttaccg gcgctttctt caccggcttg gacaccttct ttttccaaat
     2281 caccttcttc accggcttgg gcggtactgc cctttttatc gctggcgccg ccaccttctt
     2341 taccggcgct ttcttcaccg gcttggacac cttctttttc caaatcacct tcttcaccgg
     2401 ctttttagta ttttttgtta tttttgaagt taaggccata aatttagaga ctatgcagcg
     2461 agttaagctg ctcggtcaaa ttttttattt cttccattaa catcgtcatt ccttcacttt
     2521 caccagcggc ttcagccgcg ctaatccgat cttggaaaga ttttatctga cgcttaagac
     2581 taaaacgttt caattctaac aataccttag taatttccgc ttttagctga ttggcatcat
     2641 gctgataata gtctttttcc cccagaagag ccaagcgatc aataatctca gcactaccat
     2701 ctgcttgttc ttgtaaatat aagcgaaaag aatcgtaatc taaaacagca aacttattat
     2761 aatagataat caaattcttg taaaacgcaa tcagagattc gcctgataag tcgtctggtt
     2821 ctaaatgaga gacgctataa gtgataaaat cgggcgcctt cagtagtaaa gctagcagta
     2881 tttcggaaag tctttcttcg cgagtgaggc ctaatacggg agctgtcggc gttagcgtcg
     2941 ctttggtcgc agttaactct ggtcgattca cttgaacaac cggcaaactt tcccgcaaag
     3001 cctgttcaga gatgcccaga ttttccgcca gacgctgtag ccagtaatca cggtcaatct
     3061 tattggaaaa cttagcaatc atcgccagca tcaatctggc cacctccttc ttgtcactga
     3121 gcttgcgtaa atctttccca gacttcactt tttcaaaata atattccatc atcggccggg
     3181 aatttatcac cgcttgttta aaatcatcag gattagcgcg cagacattca tccgggtctt
     3241 tatactggga aggcaagaca ataatcttga tattcatatc cagcgccagc gcctctttga
     3301 tgccgcgatc agccgccaac tgtccggcgc tatccatatc gaaagacaaa atcaagttat
     3361 tagaaaaacg cttgagcaat tttaattgtt ccctagttaa agcggtgccg gaagaagcga
     3421 ccgtattcgt gaacccatgc tgatggcagg caatagcatc catctgtcct tccacaacga
     3481 tagcaaaacc ccgctcttta atggctttct tcgctttatc taaagcaaat aatacgcggc
     3541 ttttatcata aacttccgtt tgcggactgt tgatatattt acccccggta acagtgtccg
     3601 ctttttccgg attcacccga gcggtaaaag caatgagcgt gccgcttgcc tctctgatag
     3661 gaaaaatgat gcggtcgcga aaacggtcat agacgcttcc ccgctcactc ctgcccgcca
     3721 agccggcggc aaaaatttct tgctcggtaa atttctgaga acgcaaatag tcaaacatgg
     3781 ccgtccattg ttccgagccg tagcctatct gccagtcttt aatggtattt tcacttaatc
     3841 ctcttttaag aagataggct ttcattctcc ggccggtagc atcattctcc aaataattta
     3901 catattgtct agcggcgctt tccaaacaat caagcaaacg cccccgtttt gaatattcgc
     3961 cggcggctgc ctgacgcaaa actactccag ctttcggggc taaaaggcgc agagtgtcgg
     4021 gaaaacttaa tccttccatc tcctgaacga aagacaaaac atcgccgccc ttgccgcaac
     4081 cgaaacaatg ccaaatctgc ttatctgggg aaattacaaa agagggggat ttttcccgat
     4141 gaaaaggaca gagcgcttga aaattaacgc ccaccgcttt cacctggaga tattccctga
     4201 ttacctcaac aatatccagt ttagctttta tttcctcggc tggagacata tgttaagaaa
     4261 ataatctttg ccaccaagaa cgtttaggcg ctaacggcgg cttaaaagac ggcttcaagg
     4321 tcgtcaagac atcggcctga atattaccgc cggcaatagg cgccgaagaa gcagaagggg
     4381 gtaataacag tgaagagaga cgcgccgcct cctgctccac catttccccg tcataatcgg
     4441 cactatggcg ggattgacct tcataactcg ctttcttatg gtctaaatgt agattaaaaa
     4501 aatagccgcc cccttccctc tcttggacgt agagatggaa acgaggataa aaatcacggc
     4561 ttaaacgacg aacaaaactc tcctggccgc gctggcggtc attaattaag gtatagcccg
     4621 cccggcgcag ccactgcgcc ggcgaagctc ctcccagacg ctgattgtca attattattt
     4681 tcattttatt tggtctgacg aaaaatgata aaattataaa taataaaatt aatgacaccc
     4741 aaaaagatat tgacgagaat ctgagaaaat aggtaccaaa cgtggagatt gtttacaaga
     4801 atatgcatgc ccaagccgtt taaattcagg ctaataaaag cggcactaaa agaaatgatg
     4861 agttgatgag gcagatgctt atggctgtag ttacgaaaag tccagagctt ctgcaagcta
     4921 aaacttacca cgaacgaaat cagaaaagcc caaaaagtgg ccaagaggat tccccagccc
     4981 attaagccgt ggaggataaa aagaacagcc aaatcaacgg caccggcaat gccgccggaa
     5041 ataaaaaatt ttaccagcgc tttccggcga ttgcaaaaag caaaaacttt aggaaagtga
     5101 tgggcaaaaa aattacggaa atcatcgtaa aagcgattaa taagagacat atttttacca
     5161 ttgacaataa gtgaaagccc aggtggctaa agctcgcgcc tggaggaaac gttcattttt
     5221 gccgccggta tctaaaacgg cgacgatcac gctccttcca tctttgtctt gaaaacgacc
     5281 gacaaagcag tagccggcct ggtcggtata accagttttg ccgcccagag ccttaagggt
     5341 tgaagaggac gacgaatcca gaagccaatc agtcgactct aacagtttct ccctgccttg
     5401 gacagtttga aagcggtaag tatgacgggc aacggccgcg gctatttctg ctttattcaa
     5461 ggccgcctgc gctaaacgag cgacgtcgcg aacattggat aaattaccat tgctcaatcc
     5521 gaccggatcg gcaaattccg tctgtaacag ccctaagtcc ttagccttat tattcatagc
     5581 aactatgaaa tcagcagtcg acaaaccagt ggcgtgaact aaggctaaag tcgcgccatt
     5641 atcagaagcc accaagctag cattaaacaa atccttaact ttaacagtct ctcctaaaaa
     5701 aagattaatt ttaccacctt caactatatc atcgcggcgg atggtatatt ctttttccca
     5761 tccgggatta ttatctaaaa aaaccaaagc ggtcattaat ttggtaatac tggcaattgc
     5821 ctggcgtctc tccggagctt tcgccgccaa ccaaacatgg ccgtcatcgg ttagcacggc
     5881 cgcgctttta gcggcaatgc ttggcgccgg cattttccgc gccgggacat catcgttaag
     5941 aggcaggtga ccggattcaa gacaggcctc agtcccgcgt aaaggaccgc tgctataaac
     6001 aatatttacc ggcggactcc aaacagacaa gcttaaagct aacagattgg caattagatt
     6061 taacatgctt tttctgattc ggccaaaaag gcctttaatt cttcgggctg atgcaaacgc
     6121 tcataatcag gcaattcagt gacattattc aatcccaaga aacgaataaa atccagcgtc
     6181 acttggtaat aattttcatt cttctttttg tcaaattctt cttcaattaa gccgcgtaaa
     6241 agcagattac ggatgatcag gctgcagtta accccccgga tacgatccaa ttccatctta
     6301 ctgaccggtc cgcgataggc gataatggtt aaggcttcta ggctgggttg cgaccattcc
     6361 ccgcttgtct ccgccttcaa aaaggcctta atcaaatctg aattatccgg agcgctcacc
     6421 agctgataat tattagcggc actggccaaa cgccaacccc taccagattc ttcatattca
     6481 cgactgagag ccagcagcgc ctcttcgact gccgccgcct tgatgccggt agcggcgcta
     6541 agctccttta agctaagcgg cttattggca acaagtaaca gtgattctaa ctgggaagac
     6601 aggctagaca tataaatatt ttacaaaaat taaaaattat ttaaaaacaa aaatgaaggt
     6661 ttaaattttt aacactctaa tttcactaaa aagctcctct tgttcaaaaa ccaactctct
     6721 ctgcttggct aattccaaga ccgccaagat attaacgatt acttccactt tagtttccgc
     6781 cttttccagc aaacgttgaa aacttagcga cagctgttcc gccagcagtc cgcgaatatg
     6841 gctgatgcgc tcgtcaaggc tgacttttgg ctctaatctg gcttcaggta atttttcctc
     6901 ctgttttttt aaacgggcta aaatctgttg ccaaatattt tccaaagcgg aagcggtaat
     6961 ttgtttaggc ggcgcgaaaa taagctcctt gtccgccaaa ccgcgacgtc cagatttatt
     7021 aaaaataggg gcaaaagatt tatgatcacg attgatcaaa tcagaaactt tcaagctggc
     7081 ttcaataaat tccttataca tccgcagttg tctttctaaa tcgcctattt ccgcctcctc
     7141 ttcctccgaa cttaaatatg gcagaagagc tttggattta agatagagaa gacgggcggc
     7201 aatcactaaa aagtcggcaa tttcttccgg attgatattt tctgcctgtt ccacataagc
     7261 aacatattgg tcggcgatag ccgccaaagc gatttccgtt atatccatct cttcgctttc
     7321 aataagttgc aagagcagac ccaaagggcc ctgaaatttt tctacctgaa aatcaatcat
     7381 aattaatcga ttaaaatcgg ctggaccgcc ccctgatcac tttcctcctt gctccagagc
     7441 tgtcccttta agcaatcata accggcttga tgtcctttct tgactgaagg ataagcaaaa
     7501 ttctgatcac tctcatcact agctaaacga aaatgcgcgc agaaagaaaa tttattatcc
     7561 tcttcgcgtc tatactcaat cgcctgctta gtggctggat caactgtaat cctggagtca
     7621 aggtaaatat cggaatcttt aatttcttca agactggctg gcagtttacc atattgagaa
     7681 taatagctat taacagcgtt ctccaactga taaatattat taaccaaatt ctgatcaaga
     7741 cgtttagcgc gcgccaccga cggcggcgtc accaaaaacc acgcggacac aaaagcaaca
     7801 attactaaag cggccgagcc gaaggcaaaa aaacggatca ccagatcttt agcttttatc
     7861 tcttggcgct taatatcata aaaataaaaa gaaaagataa tagcggcaat caacagcgtc
     7921 gtcgccgctt taagaataaa gcgagaagtt aattccccgg acaaaaaatt attgataacg
     7981 ccgataaaaa cgccgaggat aatcatcgaa gttaccaaga tgataaaata tgtcagccag
     8041 cgacgcaagg gtgaattgag gtccaattcc tgcttgttta gaccacggtt aattaggtgg
     8101 gaaagaaaat aatagatggg agcggcgata aataaggcgg aaatagcaaa cttcaattgg
     8161 gaatcaagat tgttgtaact attataattc caagcgtcgg ccacgctctt atcaataatg
     8221 ccgaaagcaa tcattcctac cgataaagcg gtaaaaatta gtgacactag cgacagcaaa
     8281 taatagaaag cgtacttggc gttatggtta ttagtcatat ttttagactc tttccgcttc
     8341 cacgccgcca gggcgaaaaa aacggaaaga atatcaaaaa aacaaaaata aaagacagcc
     8401 ccttgattag aactttgcgt tctttggcgt gcgcggaaac ggaatcacgt cgcggatatt
     8461 agccataccc gataaataca taatcgcccg ctcgaagcca aggccgaaac cggcgtgttt
     8521 aatggaacca tactggcgca aatccaaata ccagcgataa tcctcgggat tcattcccat
     8581 ttcaacaatt ctattttcca gccattcata acgttcttct ctctggctac cgccgataat
     8641 ttcgccaatg ccgggcacca ataaatccat cgccgccacc gtcttgccgt catcattctg
     8701 acgcatataa aaagccttaa tgtctttagg ataatcagtt aaaaatatcg gcttcttaaa
     8761 aatcttctcg gctaaataac gctcatgttc ggtctgcaaa tcaatacccc aggaaaccgg
     8821 ataagcaaaa ttttcgccac tctttagaag caaatcaatg gcttcggtgt aagtcacgcg
     8881 gccgaaatca gccgctgcca ccgcctttaa tctctcaatc agccagttat cgataaactg
     8941 atttaaaaac tccatttctt ccggcagttc cgtcaacacg tagttaatta aatatttcag
     9001 cattctttca gctaaatcca tgtcgtcttt caattccgca aaagccatct ctggttcaat
     9061 catccaaaat tccgaagcgt ggcgggtggt attggaattc tctgcccgga aagtcggacc
     9121 aaaagtatag actttattca aacccataat caaagcctcg gcgttcaatt gaccgctaac
     9181 ggtcagcccg gccttctgtc caaaaaaatc tttaccaaaa tcaatctctc cgtttttttg
     9241 catcggcact ttctttaaat caagcgttgt tacttggaac atttcccccg ccccctcaca
     9301 atcactggtg gaaataatcg gcgtctgaac ataagcgaat tcctcctcct gaaaaaattt
     9361 atgaatcgcg tagcttaaag cggaacgcaa acggaaaacg gcagaaaaag tattgctgcg
     9421 cgggcgcaaa tgagcgattt cccgtaaaaa ttcaaagcta tgtttttttt tctgcaaagg
     9481 ataatcttct ttggcttcat taataaccgt aaccgaaaca gccgacaatt caaaagcctg
     9541 accggcggcc ggcgaaggaa cgagaacacc ttttacttcc aaagaagaac cgatattcaa
     9601 tttctccaaa gctgcaaaat ttggcaatcc cttattaatc acgatttgta aacccttaaa
     9661 aaaactgccg tcattaagct cgataaaagc gacatcgccg gaagagcgca ccgtgcgtac
     9721 ccagccggca actaaaattt ccttattcaa aaattcatcc gtgtgtcgat ataattgttt
     9781 gcttgtcgta tacatatatt taaatgatta agggcgtaag cgcttggcgt cgcgcggcaa
     9841 gaaacaagct tccttaacat tatcggcgcc aacgattttc atcactaaac gggccggacc
     9901 gatgccgacg ccgccatgcg ccggacagcc gtaacgaaaa aaattaaaat aatcgtgcag
     9961 ctcttccaga ttcatttctt tctctaaagc ctgcttttcc aaaacatcaa ggcgatgttc
    10021 gcgctgagag ccggtcgtaa cctctagacc acgataaagc aaatcaaaac tcttagtcaa
    10081 accaggatta tcggcgtggc gcatgtgata gaaaggacgg gcagcaatgg ggtagtcggt
    10141 gacaaagatg aaatcatgat taaattcttc tttaaccaaa cgacaaattt ccctttcttc
    10201 ttccggcgac aaatcatagt ctttctcact cttaatgccg gctgctttca atttttcttt
    10261 gacttcagcc aaactcatct tgggaaaagg cgcggcggga atctcaatat caagaccgag
    10321 ctcttttact ttaccaaaag ccctaatcaa ggtccgctct tccaaagcca tgatgtcatg
    10381 atgcgattca atatcggcaa attcaaaatc ccagccggta aattcagtga catggcgact
    10441 agtataggaa gcttccgccc gaaaaaccgg accagtcata aaaacctttt caaagccgga
    10501 agcgatagcc atctgcttat agaattgcgg cgactgggaa agataggctt ggcgatcgaa
    10561 atattttact tcaaacacct ccgagccggt ctcactggcg gtattcatga aacatggagt
    10621 ataaatctgc ataaagccct cagcgagtaa ttgttcgcgg aaaccgactt ctaactgcgt
    10681 ccatacctga aaaatctgcc gatgctcggg acggcgcaaa tccaaccagc gccaatcgaa
    10741 acgattatca atattggctt cgttatctat cttattaagc accggaatcg gcagattcgc
    10801 ctcagctaaa ctaataatct ccagagaaga aaccgataat tcccaatctt taaccggatt
    10861 aggatcgttc ttcttcgccg gcttctcttt caattctccg accaccttaa taaaactttc
    10921 caaagttaaa gcttccgccg ctgtaaaaga ggcgccgatt ttttcttcca ccaccaattg
    10981 catattgccg ctagagtcgc gcaaatcaat aaaaaccaaa cccctcatta atctgatatt
    11041 ttgaacgaag ccatgaagtt caaccttgcc aagcgcctgt tctttagcag ctttaatgct
    11101 tatttttgac atattttcaa aatttaatta taaaaatata taactttccc tctatttttt
    11161 accaactaat cttaccgcat ttaaggtctt ttagcaagtt gaaaaaatta gaattctgcc
    11221 taaatttttt taaaaaaata ttgacaaaaa atctattttt aattaataaa gccaagaaat
    11281 aatttgaaaa tttaagttct cccactcctt aagaatttta ggcgaaaact ttaaattttg
    11341 ccaagtatta cccgaagcca aacgtaataa tttaatgcag ttatcagaaa ttggaaccat
    11401 tgaatcaggg gctgatattt ttaaatttga cgaataacag tcagggcaaa gcaggccgcc
    11461 gcgattgcag tcgaaataat tattaccagg cttaattgcc gctttgcagg cagtgcagac
    11521 atcgagccgc ggttcatgac caagaagctt gaataaacgc cacaaaaata aagccaagcg
    11581 aaaacggcct tcatctttat ctagactacc cttctctccc gccgcctcta gacttagtaa
    11641 ccatttttcc aaccaagaaa aaagattcaa atccgcttcc ccttcttgga ctaaaatact
    11701 aaaattacgc aaggcttcac cggcaaaata taatttattt aaatcctgct taattcctaa
    11761 aaaaatttct tccgacaaag cggcgccgac ataatcgaag ccttttcctt ttactactaa
    11821 taatcgactc agactaatgg gctccaaatg cgccgccagt ttcgaacccg ccttgcgcgc
    11881 tccccgtgcc actaaagaca gacgaccgta atcaggcgta taaacagaaa cgaggcggtc
    11941 ggcctcacgc caatcccgcc tgtttaaaat aatcgctcgc gtcggaaaag tagaatccat
    12001 aaattattta ttctcctgat ctaaataatt ttgtaaaaaa atagtggccg caatttcatc
    12061 tctgcccgcc ttatcgcgct tgctccccgc caaagcgtct gccgccttgc tggacaagcg
    12121 ctcatcgacc gttaccaccg ccacggtcga ttttgacttt aattccttta aaaaatgtaa
    12181 aaataacgga ttggaagcct cgtctcccgc cattttacga ggagagccaa caaccactaa
    12241 agcaatggac tcggcttcaa ttattgccaa aagcgagcgt aaatcagtga cagtcataaa
    12301 aggcaaggct aagcgagttt cggcatcagc caaggccagg ccgattctct tctctcccca
    12361 gtctaccccc aaatattgct ttccttcctc cattttcatc ttattccaaa gttaaaattt
    12421 cataagcgtc tttagtaacg gccagcgtat gttcaaaatg ggcgcttaaa gacttatcgg
    12481 cagtgacaat ggtaaaaccg ttttctgcca cctcaatctt ataagttccg gcatttatca
    12541 tcggctcaat gcaaattacc atcccctctt tcaattctat attagcggcc gaacgttcgc
    12601 cgatgcgata atgatatatc tccgggtctt catggacttg atagccgacg ccgtgcccga
    12661 ctaaatccct aactacaccg taaccggccg tttccgccaa actttggaca gccccgccga
    12721 cattattaag acgcgccccg ggtcgaacct gtttaatccc cgcttccaaa cactcccgcg
    12781 tcactctcaa caatcttttg gcttcccggc taatctctcc gaccggcacc gtcgcgcaca
    12841 tatccgtata aaaaccgccc tccggagagt gaggattaag cggcaagctt aatttctccc
    12901 ttaactccgg ctttaccggc cattccatac cgatatcaag atcaataata tcgccggctt
    12961 taagaatacg cccggggata gcggccccat gaacgacttc atcattaatt gaagcgcaaa
    13021 tagcggaagg aaaaaaaata ccgccaccca tcggataatc cttaaaagcc ggccgaccgc
    13081 cggcggccgc taccatcgcc agcatcattc cctccaaatc agccgtatta actccgggct
    13141 tcaccgccgc ttttaatctt tttaaaatag aggccaaaat cttgccgccg gcgcgaatag
    13201 cttcaatttc ttcttttgtc ttaatataca ccataataaa attaccaaac taatattttg
    13261 gccaagcggc tgtttctttt tttacttaaa actctccaaa atttctcgaa tatggtcaca
    13321 atcaacttca gtcaatttta aatcgtatct ctgccgatat aacttataca tatcgcgatt
    13381 aattacgtat tgggaaacac ctttaccggt acgcaatttt tctttatatc tcttgaccaa
    13441 agccaaaact atttccttat tctcagaact aagatgctgg aaatgataat tttcccgcac
    13501 cagttcgttg agcttgcgct caataatatc gctgggctca tcttttatat tagccaaaaa
    13561 atctttcaaa tccataggtt taagaattta caagctgatt atatcataaa aatgtagctt
    13621 tcgctacatt ttttaaatta tttttttaat tataaaataa taaaatataa ttcggccaat
    13681 taaatcccat catactctct cattgtcagc tgcgactcaa tctgtttgac cgtttcaata
    13741 actacggaaa caacgataag caaaccggtg ccgccgatag ccatcgactg gataccggta
    13801 aaataacgca ttactaacgg caaaacggcg ataaggctta aaaacaacgc gccggcaaaa
    13861 ataatgcgat gggctgtata cgctaagtaa tccctagtcg gtttaccggg acgaatgccg
    13921 ggaataaaac cgccctgttt ctgtaaattc tcggaaattt gatcaggatg gaaaataact
    13981 tcagtataga aataagtaaa agcgaaaacc aggatgaaat aaataatgcc gtaaattaac
    14041 tgattgttaa agatttcaat caccttagca gagaaattgg caatccaggc tgtgcgggca
    14101 tgcaagaaga attgggcaat catcggcgga aacaagacga cggaaatagc gaagataatc
    14161 gggataacgc cggccatatt aacgcgtaag ggtaaatgag tactggtgcc gccgaaagcg
    14221 cgattaccgc gaatttggcg ggcatactgg accgggatat tgcgctgacc ctcattgaca
    14281 ataacaacgc cgatgacagt taaaatagcg acaaccacga aaccaacgac ggtaaatatc
    14341 tgagtctgat caaaagtcaa gataaattgc tgaagctgtt gccaaatacc agcgacaata
    14401 ccggcaaaaa ttaaaaggga aatgccgttg ccaattttct tctcagaaat taattcacca
    14461 atccacatta gaaaaatagt gccggcggta atggttaaaa taataacacc aaaatcaaaa
    14521 gcgctgatat cgcctaatat accttggttg gaacgctgta aaagcgtaat catgccgtaa
    14581 gcttgcatgg cggccaaagg cacggttgcc cagcgggtcc acatattaat tttctggcgg
    14641 ccgctttctt ctttctgcat ttcctcaatt ttaggaataa tcatgcctaa aagttgaaaa
    14701 ataatagaag ccgtaatgta aggagcgatc cccatcatca cgatggaaaa attttccatg
    14761 cccccgccgg agaaaatatt cattaagcct aatatctcat tagaggcaaa aagattcttc
    14821 aaagccgcag tatcaacccc tggcactgga atatgggccg ccaagcggaa aactaccagc
    14881 atggcgaaca caaacaatat gcttctgcgc aaatctttcg ctttccaaat ttgttctaat
    14941 tttgccaaca taaatttaaa ttactaaata taggcaaaag ccaaatatcc taagcggata
    15001 tctggctctt gacgccggcg ctcatggcaa ccttctcgaa tttgacttta atcgttaatt
    15061 tttccttccc taaaatttta accggagaag agacgttctt gattaaacct gcctgccata
    15121 aagattctgg agtaatgaca tcgccgtcat gaaaattctt attaatcaag gcaacagaaa
    15181 caatctgatt cttcggctgg agagaacgga aaccgcgtaa ttttggagtc tggagtaatt
    15241 gcttgcgcat cccgagacgc ttcaagccgc taacaccgga acgagctttc tgccccttta
    15301 aaccacgagt actgtaggta ccgtggccgg aagcattgcc gcgaccaacg cgtttcttct
    15361 tatgggtagc gccggaagag cgcttaatat tgttcaatga caacataaaa ttatttttat
    15421 ctttaaaatc tgcttttatt tcttggtcgc tgcttttttc ggagcggcct taacagtttt
    15481 ttcttccttt acttcggtct tcacctcttt tttctcctta gtcttaacgg atttcttatc
    15541 tggctggcgc aggcttgcta aagcggcgaa agtgcagcga gcattattaa tcttattgtt
    15601 agtgcccaaa actttactgg tgacgttttt gatgccggct agttctaaaa ttgcccgaac
    15661 aacgccacca gcgataacac cgcgtccctg ttgagccggt ttgaaaacga taagggccgc
    15721 gcctaatttc tgacgaacaa catgaggaat agtgtcatta actatgctca cttttaacat
    15781 ttctttctga gcgcgattaa ccgccttatt gacggccagg gtaacatcag cgcccttgcc
    15841 caaacccaaa ccaatattgc ctttacgatc accaatggcg acacaagcgc gaaaattcat
    15901 acgcttgccg ccggccataa cgcgagtaac ccgggcaatc tctaaaatac gctgttcctt
    15961 ttcgtctttg cgctcatcac ggctaccgcg actatcgcgt cgcggaccgc gtccggaagc
    16021 gccgcgctga cgtttttcct gacctccgta aatgtccttg gctaccgccg tggccacctt
    16081 attttccgga gtatcagctt ctacttgttt gttatcgtct gccatatgaa aaatcttaat
    16141 acttatctaa aaatttagaa aactaatcct tgctcccgag ccgcctcagc caccgcctta
    16201 acacgaccgt gatagcgata ggaactgcgg tcaaaaactg cttctttgat acccaaagcc
    16261 ctggcctttt ccgctaaaat tttacctaaa gcggcagcgg cttccacctt cttcaatttt
    16321 ttgtctttta attctcgaac atgagcactg gctaaagtgc gaccggaaac atcatcaatt
    16381 aattgtagat aaagaccccg attagaacga aaaacattaa gacgagggcg agaagcggtg
    16441 ccactaatct tagcacgcac tttatcctgg cgacgctgtt ttctggcttt ttcttttttt
    16501 ataatattca tcatatattt ttagcaaaag attactttac ttcttagcag cggtcttacc
    16561 ggccttgcga cgcaggacct cgtcgacata tttaacaccc ttacctttat aaggttcagg
    16621 aacgcgtaat tttctgattt taaaggcagt gttgcccaac aactcattat caatgccgct
    16681 taaagtgatg acattagctt cgacggtggc actgatgcca gccggtaaag gaaattctat
    16741 cgggtgagaa tagccgagat ttaaatttaa cttactgccg ttgacggcta cccgataacc
    16801 gacgccatta atctctaatt tcttagtgaa accgacttta actccggtta ccatattatt
    16861 aactaaggcg cgatataaac cccaataagc actggcatct ttagtgttct gagtgacgac
    16921 aattctaatg gccgcctcat cgatttctac tgtaaccgga gttttaaggg cgcgagacag
    16981 ttctcctttc ggccccttaa cggtcaaaac gccgttatta aattgagcgc tgacgccttc
    17041 tagtaatttg atgggtaatt ttcctaaacg agacatattt tccgaagcgg tgatgatcca
    17101 gcctgagacc ggaacctaac cgcgaaatta gttagtacac ttcgccgacc acttcgccgc
    17161 ctattttctg acgataggct tcacgattgg tcattaaacc agaagaagtg gaaataatcg
    17221 ccataccaaa attattcaaa acggtaggga tgttttcttt accgacataa actctggaac
    17281 cggccttaga gatgcgtttg atgttactga taaccgggtt gccattcttg tgatatttca
    17341 aaacgatttt taattgatca aattggctgc ggcgcgcatt caagccaccg gcaacgattt
    17401 cagcgtggct aatccagcct tctctttcca aaattttggc gatttcgtat tttattttac
    17461 tcatcggcat taaaacttcg gcttgcttga tggcggaagc gttacggatg cgtgtaaaca
    17521 tgtctgcgat tgaatctgtc atattgttat actatgttta gataatatgg gcggccggaa
    17581 tttaccagct agatttggtg atacccggca ggtcgccgtt atcagctaat tcgcggaagc
    17641 agatgcggca caaaccgaag tcgcgcagat agccgtggtt acgaccgcag cgccagcaac
    17701 gattaatctt gcgggtggaa aatttcggtt tgcgcttcgc tttgacgatg agagctgttc
    17761 ttgccatatg atatgtaaat tattttttgt tctttttaaa aggaaagccg agagcctgaa
    17821 ataaagctaa accttcctcg tagttcttag cgttggtgtc caaacaaatt tctaagccat
    17881 aaatctgatc aatatcttcc gccttcaatt caggaaaaca ggaataatct ttcaaaccga
    17941 tggtcatatt accgttgcga tcaacacttc tatcactgat gccccggaaa tcacggacgc
    18001 gcggaaaaga aatattcacc aatttttcta caaaatcata catcctctca ccgcgtagag
    18061 tcaccaccgc accgacaacc atcccttcac gaaccttaaa ggcggaaatt gattttttgg
    18121 ccttggttaa aatcggcttc tgaccgctaa ttttagtcaa agcggattca atcgcgctaa
    18181 tagcctcttt ttccttagcg tgtttaccga agcccatatt aattacgact ttctgcaaac
    18241 gaggaaccag gatggcattt ttataagaaa acttctcctt taagctaggt acgatttctt
    18301 tttgatattt ttcttttaaa cgcataaaat tggaattact cgattacggt ctggcatttc
    18361 ttgcagaccc gatatttttt gtttcccttc tctccggcaa caatcttggc agcaaccctc
    18421 gtttgacgtc cacaagaagg acatattaaa gcaacgttac tcgccttaat aaaggccgga
    18481 aattcaatgc gctggccttt ttcaccttta cggctggggc gcatatgttt aatcagcaaa
    18541 tttaaacctt cgatactaac cttatcttcg gccggaagga cttgcaaaac tttaccggtt
    18601 ttacctttat ctttacccgt taatatttta acagtgtcat ttttcttgat gttcatatat
    18661 attcaaattg gcgcttttaa ttataaaacc tcgggagcca aagaggcgat tttgacaaaa
    18721 ccagcacgac gcacttccct ggccaccggg ccaaaaatgc gagtaccctt cgggtctttc
    18781 ttatccttgt cgacgataat cacacaagca ttgtcgtcaa aacgaacgta agtaccgtct
    18841 tgacgcctta tttccttgtt ggtgcgcaca ataacagctt taaccacatc gctctttttg
    18901 acagccgcat gcggcaaagc gctcttaacc gtgccgatga tgatatcgcc gactcgggca
    18961 taacgcttgc gatagccgcc taatacgcgg atacacatta tttctttagc gccggaatta
    19021 tcggctactt ttaattttgt ctgtacttga atcatactaa tatcgttaat ttccgccctc
    19081 tcataaagga cggataagcc aagctgcttt agttctcaat tatccgccaa cgcttgttct
    19141 tgcttaaagg gcggcattcg ataaaggtaa ctttatcgcc ttctttactc ttattttcct
    19201 catcatgcac ctgatattga cggctaaccg tgtaacgttt ctgatatttc tgattcatct
    19261 taacggtatc aaccttaacg acggcggtct tgtcgccttt agcgcttaca actactccgt
    19321 gaaaacgctt gataatttta ttgtcggcag agatagcctt tgtttgtttt tgggcaggca
    19381 taaaagttta agttaagctt tggtgttaag caccgttaaa attctggcga ttgtttctct
    19441 aatctgcctg atttcacgga tatttttgag ttgacgatta gaatctttaa agcgcaattc
    19501 acgcagcttt tcacgggatt gagccaacag agcttgtagc tcggacggat ttttagtttt
    19561 aagctctttt atttccatat tattaataaa taattgctcg agtggtggct ttttatttgc
    19621 taagcacctt gcatttaacc ggtaatttat gaccggccaa agtcatagct tcgcgagcgg
    19681 ttttttcatc aatcccctca atttcgaaca taaccgtacc gggcttgacg acgcagacat
    19741 agtgatcgac tgcgccctta cctttaccca taggcatttc cccgccatga gcggtgaccg
    19801 gcttatcagg aaaaattcta atccaaagtt tcccggtctt cttgatataa cgagtcatta
    19861 ctctccgagc cgcctcaatc tgacgagaat ttatccagtg tgccgtcatc gcttttaggc
    19921 cgtagctgcc aaaattgaca tcgaccaaac gcgtggcgac accgccgcga cggcgtttat
    19981 gatctttacg gtatttagtt ttcttaggca ttaacatatc ggtatttaaa tcttttaatt
    20041 tttatttaag cggcaatcaa ttctcctttg ccgtcttttt ctttttcaaa tatttctcct
    20101 ttgtaaatcc aaactttgat gccaatagcg ccgtagctgg tgtgagcgac gccgcgcgca
    20161 taatcaatat cggcgcgcaa agtctgtaaa ggcactttgc cggaaaccaa tttttcggta
    20221 cgcgcaattt cggcaccgtt taaacggccg ctaatctgca ctttgacgcc taaggcgccg
    20281 ccgcgctcaa cccggctaat aagctgcttc attactttac ggaaaggcac gcgcttttca
    20341 atatctaaaa tagcggactg aacagcaatc tgggcgctta attgaggacg attaacctcc
    20401 ttgatattaa gattaatttc acccatgcgg aaattacgca aaaactgatc atgaattttc
    20461 ttttttaaat cttcaacacc attgccgccg cgaccgataa tcaaaccggg cttagcggtc
    20521 caaatattaa gagtgatttt atttaagctg cgcactattt ctactttatc aataccggct
    20581 tcgcgcattg tcttaatcag atgcttgcga atagcgatat cctgctggac attcttgata
    20641 taattacggc cgctttcaaa ccagaccgat ggccaagtct tgatgacgcc taagcgcatt
    20701 acttttggat ttactttttt acccatacta ttggttatgc gtgattaatt tgctaaatta
    20761 tttgcttccc gccttacgat taaaaatttt agtggtgaat cctttaccgg atttcgattc
    20821 agtcttggtt ttagcggtat tcttaacagc ctcatctttt ttcgcgccgg ctttagagcg
    20881 tttttctgcc tcggcggcta tttgctctaa tttaaccgga gcctcaactt tactcttacg
    20941 ggcttctttc ttaccacttt ctactatttc cgccaaaact accttgaccc ggcaaccgcg
    21001 ctggcggata acagtcgcac gaccatgagc cttaggcatc caacgcttta aagttaaacc
    21061 gctatcagct ttaatttcct taattaataa attacttctc tccaggctga aattatgttc
    21121 agcattagca agggcggatt caattaattt cttaatcggt gcggccgcca atttattcaa
    21181 aaactgcagc tgttccaaag ctttatcaac ctgaaggccg cggacaacat caagaaccaa
    21241 gcgcgctttg cgcggagaca ttctcaaatg atttaaacta gctttaattt ccatagaatt
    21301 cacgtgttgg cagaatcagc gcattggacg ccaatcgcct cgcattttaa ttatttgtcg
    21361 ctcttagccg gctgagcctg agccttagcc atcttgccgc catgagaaac aaatttcctg
    21421 gtaatagcaa attctcctaa acgatgacca accatgttct ctacaatata aacaggaaca
    21481 tgctgcttgc cgttatggac accgatggta aatcccacca tttctggaac aattgaacag
    21541 gcgcgatccc aaaccttaat aatagtctta tcgcccggtt tcaaagcctg cactttcttc
    21601 aaaatgcgca gattaacata agggcctttt ttcaaacttc ttgacatatg cttttaaata
    21661 ataggtgatt atttattctt gccgcggcgc ttgattatca atttattaga agagcgctgg
    21721 cggcgggtct tgacgcccag agcaggttta ccccaaggag ttttaggatg atgaagacca
    21781 atggattgat tgccttcacc gcctccatga ggatgatcaa ccggattcat cgccgtcccg
    21841 cggacagtcg ggcggatgcc caaatgacgg ctgcggccag ccttacctaa aacgatgtgg
    21901 cgtttatcag gattgctgac ttgaccaaca gtacataaac attctttctt gaccaatcta
    21961 atttctccgc tcggcatttt tacctgagca tatttgcctt caacgcccat cacaaaaacg
    22021 gagttaccgg caccacgggc aattaaagcg ccgcggccag gttctaattc caaactgtta
    22081 atagcgacgc cggccggaat atactgaaca ggcatagcgt taccattttt aatttcgatt
    22141 aagttcttag aactcaaaag tttatcaccg acaactacgt tgaccggagc aatgatatag
    22201 cgcttttcac cgtcggcgta atttaataaa gccagacgcg caccccggtt aggatcatat
    22261 tcaatagcag tcacgatagc cggaatatca aatttatcgc gcttaaaatc aaccaagcga
    22321 agatacctct tctcgccgcc gccacgatga cgaacggtga tttttccctg gctgttgcga
    22381 ccactggttt tcttcttcct gacaattaag cgcttttccg gaacatgctt agtaatatcg
    22441 gcgctgtcat cgaaagtggc gccgcgacga ccgggagtat tggctcttac ttttttaata
    22501 ctcatatata atcagttact tgtttataaa ttaaacgcct tcgtaaatct taatgctctc
    22561 gccctttttc aaagtgacga ttgccttttt ccagtccttg cgctttccct ggatgcggcc
    22621 acgagtaacc gctttgccct tcatggcaat aatattaaca cttaagactt tcactccata
    22681 gacagcctga atcgctttag ctacttcaat cttattagcg gcctgggaaa taacaaaagc
    22741 atactgattg tgcgcactta attccgttgc tttttctgta attaaaggtt taactaaaac
    22801 tcgaaaagca ccgtcataac gagtagcgac agaggctttc ttagcacgcc caaacttctt
    22861 gtcctgatta tcagcggcat agagcgcttg catactcacc tcagtctcag ccgaaacagt
    22921 ctttttttcc tctttgacag tcgttgcctt cttttctttg ggggcggcag tctttgtctt
    22981 gttcttttgt tttactccaa ataaagccat atatttgttc aaattcaaaa atgaaaaatt
    23041 aattcttact atacctcgct gccaaagtct tgagactctc ttcattaatc attacttgac
    23101 ggcaagtcag caaagcgaca atattgatat tttctagatt aataatatta acacccttaa
    23161 gattgcgacc ggaatagaaa gccttttcgt ctttctcgcc attgatagcc aagagacaac
    23221 ggcgattggc cggtaaaata tctttttcaa aagccctaat catgctatta aactgtttgg
    23281 tcttgaattc agccatcttc aaactatcaa taactacgag actagactgg gcaatcttat
    23341 cagacaaagc catcataatc gccttttgtc tcatcttctt atttagcttc ttagaaaaat
    23401 tacgttcttt agtggggcca aaagtaaccc cgccgcctct ccataaagga gaacggctgg
    23461 aaccgacacg ggcacgtccg gttcctttct gtttccaggg ttttttgcct ccgcctctga
    23521 cctcgctccg atctttggta tgcgccaaaa cttgacgttc attggcttgc tgagcaacca
    23581 ccgcttgatg cagaagatca gtgttaactt gaagaccgaa caccgcctcg tctaattcta
    23641 aatccttaac gggaacggcc ttctgattat aaacttttac tttaatagac atatgattaa
    23701 aaccgggatt agtttgattg cttaaacggc cggagcctct tctttaatgc cattaacggg
    23761 agcggcttca gccaacacct ctccggtttt agactcttcg accggagttt cttcaacctt
    23821 tatctcctcg gccttagact cttcaacaac cggaacggcc ttaaatatca attcaccttc
    23881 acccttaatt agcaacaaac cgtcattagc gccaggaaca gctcccttga tatataaaat
    23941 atttttttcc acatcaatag cggcgatgga taaattctga attgtcaccc tttccttgcc
    24001 catgcgtccg cccatgcgca tacctttaaa aatatgagcc ggacccttag gaccaaccga
    24061 accaggcatg cgtaactgat ctttattgcc gtggctctta cgtccgcctt gaaaaccatg
    24121 acgcttaact acgccttgga aaccacgtcc cttagacgtg ccggtaacag aaacaacatc
    24181 gccaaccgca aaagtagaga catcaatctg atcgccaatc ttagcatcaa tatcattttc
    24241 ggttctaaat tcacggacat gcatcggcgc tacgcccgct tttttaaaat gaccaagctg
    24301 cggttttttg atattctttt ctttacgtac acccaaagcc aactgtaggg cggaatattt
    24361 atcaagtgct tttgttttta cttgagtgac tgtgcagggt ccggctaaaa ccggagttac
    24421 cgccaaaacg cggtcattgt cccaaagctg agtcattggt aatttttttc ctaaaataaa
    24481 tttcataatt tttaaaacaa aaaactggta attctaccag cttaaaaaac aaaagctaaa
    24541 attgcgccgt tcctccctgc tctcaatttt tttcttgttt tttggctgtt ttctcctaat
    24601 ttgccaccta tttgcccaga tacactttct ttggcttaag ccaaagagtt ataaccgggc
    24661 gcctaagtga cgcttaatca atatttgctt gtattttact gcatttttat ctccacgtca
    24721 acccctgccg gcaaattcaa atttgtcagg tcttcaatcg tttttgcccc gggatcaata
    24781 atatcgataa tgcgtttgtg aatgcgcatt tcatattggt cacgagcgtc tttatgaacg
    24841 aaagtggaac ggttgatagt gtacttgcgt ttttctgttg gtaatggtat cggaccgaaa
    24901 aaactggcgt cactgcgttc aatagtatca acaatcgttt tagtagcctg atcaataatt
    24961 ttatgatcaa aagctttaat cttgatacgg attctctgtt tgtaatcctt ttcctctttc
    25021 ttgggttcag acatagaaaa atgtacgttt atatttatag tttatattta taataattaa
    25081 ataaacacta caagctcgcc cttttgaaaa agagcaagcc taagtgccaa cttacttaat
    25141 aatcttgacc acagcaccag caccgacagt acggccacct tcgcggatag cgaagcgttg
    25201 cttctcttcc aaggcaaccg gggaaattaa cttaacggta atagtaacgg tatcgccagg
    25261 cataaccatt tcagtgcctg ccggcaattg cacttcaccg gtgacatcgg tagtgcggat
    25321 ataaaactgg ggcttataac ctgtgaagaa aggcttgtgc cggccacctt cttctttgga
    25381 gagaacataa atctgagcct cgaattcagt gtgaggagta acgctgcccg gtttgcataa
    25441 aacctgaccg cgctcgactt cattcttctt ggtgccgcgg agtaataaac cggcattatc
    25501 gccagcctcg ccgcgatcca aagacttgtt aaacatttcc acgccaatga tagtggtctt
    25561 cttggtttcc attaaaccga cgatttccac ttcatcacca atcttaatca cgccgcgctc
    25621 aatgcgaccg gtaacgacgg tgccgcgacc ttcaatggag aaaatatctt caatgggcat
    25681 taagaaaggt tgatccaggt cgcgaggagg caagggaata tattcatcta actgcttcat
    25741 taattccatg atcggttcgg catcgggacc ggtcggattt tctaaagctt tcaaagcgct
    25801 accgcgaata atcggagtgg tatcgcccgg gaattcatat ttcttgagta aatcgcgaac
    25861 ttcttcttca actaagtcga taagttcctt gtcttccacc atatcgcact tgtttaagaa
    25921 aacaatgata tagggtacgc ctacttgacg agccaaaacg atgtgctcgc gagtctgagg
    25981 catagggccg tcagtggcgg aaactactaa aatagcgccg tccatctgcg ccgcgccagt
    26041 aatcatgttt ttgacataat cagcgtggcc gggacaatcg acgtgagcat aatgacgagt
    26101 cttggactca tattctacgt gagcggtggc aatagtaata ccgctttcac gctcttccgg
    26161 agcggcatca atttcactaa cgcctttttt agaagcgttc attcctctag cgccgaacac
    26221 cgctaacatc gcggcggtca aagtggtttt tccatgatcg acgtggccga tagtgccgac
    26281 gttgacatgg ggttttgtgc gttcaaattt gtctgccatg ttgtttatac ttacggcccc
    26341 ctaaaggggt ttctttccta tatagaggaa accagccgtg aaattaatta atatttttaa
    26401 atttataatt taaacttatt tatcatgatt ttccgcttta tttttaatct gaggggggat
    26461 attccaattc cttctggatt tgctttcttc agctaaatat tcagattgtt cttgattttt
    26521 ccagtctgag atttcgcgat ttattttatc agctaaatct tcttctgtca aattaggctt
    26581 ataaattgct tcttttttat ctaaagttac agaatcaact attttttcat aacgatctag
    26641 gtcaataatg gcaaaggaat cttctggggt ctcagcgtcg aagaaaataa tgggattatt
    26701 agtttttcgt gctaatttta aagctttttt taattgttcg cgcataattt cgcaccgccc
    26761 tccttagcgg cttaattatt tacccgattt ttcctttatt gtttccagaa tattgcgagg
    26821 gacttcacta tagtgagaaa attccatgac tgaattagct cggccctgag acatcgaacg
    26881 caaagcggtc acatatccga acatttccgc caaaggcact ttcgctttaa tcgctttaat
    26941 tttattaggc ctatcaatca tttcttcgat ttggccgcgc ttggaattaa ggtcgccaat
    27001 aatgttaccc atataatctt ccggagtggt tacctctacg ttcataatcg gttccaatag
    27061 aatcggcttc gccttctgac aggcatcttt aaaagcaaat aaaccggcca tcttaaaggc
    27121 gatttccgaa gagtcaactt catgatagct gccataataa acagtcacct taacatcaac
    27181 catggggtaa ccggcgacaa caccggacaa caaaccttcc ttaacccctt tttcaatagc
    27241 cggaatatat tctttaggga taacgccccc cttaacttca tcaacaaact gataaccctg
    27301 acctttacca gatggttcaa cccgcaacaa acaatgccca tattgacccc ggccgcctga
    27361 ctgcttgaca tatttatatt ccgcttccgc tttttgagta atagtttcac gatacgagac
    27421 ttgaggctta cccacattcg cttccacacc aaattccctc ctcatgcgat caacaataat
    27481 atccaagtgc aattcaccca taccggaaat aaggatttga ttggtttcct catcagtttc
    27541 aacccggaaa gtcgggtctt cttccgccaa acgggagaga gcgacaccca tcttttcttg
    27601 gtcagcttta gtttttggct caaccgcaat cttaataacc ggttctggga aagtaatcgc
    27661 ttccagtaaa actggtttat tttcatcgca caaagtattg ccagtggtag tatttttcaa
    27721 accaataaca gcggcgatat caccggcata aatttcctta atttcttcac gataattagc
    27781 gtgcatgcgc actaaacggc caatgcgttc cttgttaccg gtagaggcgt taataatgta
    27841 agaaccggct tgcaaaacgc cttggtacac acggacgaaa caaagtttgc caacaaaagg
    27901 atcagtagca atcttaaaag ctaaaccgac aaaaggagca ttatcatcag ctttaactat
    27961 tactttctta tcggcgtcat taagatcgct tgcttccaca tctttaacat ccaaaggtga
    28021 gggcagatac tcattaacag catcaaggac caactgaata ccgatatttt gcaaagcagt
    28081 gccgcataaa accggataaa ttgtattatt taaaacacct tgacgaattg ctttttttaa
    28141 atcagccacg ctgatttttt cttcgtttaa atatttttcc atgattactt cgtcgcactc
    28201 gaccgccctt tccactaaat cagaacgata tttttccact ttttctttca tgtcttcggg
    28261 aatttctact tcagtaatat tctcaccaaa attaccagta aaagtataag ctttacatgc
    28321 caataaatcg atgacaccct tcagattatc ttccgcccca ataggtaatt gaatggcgac
    28381 agcctttgaa tttaaacgtt gtttgatgga atcaaggctc atgtaaaaat cagcccccat
    28441 tttgtccatc ttattaataa aacagaggcg gggtacgtga tacttatccg cttgacgcca
    28501 aactgtttct gactgcggtt ctacgccggc ttgaccgtca aaaacagcca cggcgccgtc
    28561 cagcacgcgt aaagagcgtt ccacttcaac ggtaaaatca acgtgtccgg gagtatcgat
    28621 aatattgatt ttattaccct tccaaaaaca agtagtagcg gcggaagtga tggtaatccc
    28681 tctttctttt tcctgttcca tccagtccat ttctgcagat ccttcgtgca cttctccgat
    28741 tttatgcttt tttccggtat agaataaaac ccgctcggta aaagtggttt tgccagcatc
    28801 aatatgagcc atgatgccga tatttctgat tttatcaaga gcgtaatcac gaggcataaa
    28861 atataaaaac taaatatgta aaaaattaaa tttgtattaa caaaattacg tcggaaatag
    28921 ccgaaagtct gggccaaaaa attttagcga gaaaaatgag caaaggcctt atttgcctca
    28981 gccatcttat gaaccgcttc ccttttcttc acggccgcac cttcaccatt ggcggcgtct
    29041 aagatttcag cggccatctt ttccgccata aagcggccgc gacgagcatg agcggcggta
    29101 atcagccagt gacagccgag ataaaaacgg cgttcgcctc gtacctgaaa aggcacctga
    29161 taattggcgc cgccaacgcg cttaccgcgc acttcaacta aaggagaaac cttcttcatc
    29221 gctttattaa aaacatggcg agggtcctgt ttggtctttt ctttaattaa attaaaagag
    29281 ctatagacga ttctctcggc tacgcttttc ttgccatccc gcatcacata attgatgagt
    29341 ttggtaatat ttttatcgtt gtatttgccg tccccttcaa tgtctctttt gggggcttgt
    29401 tttcctctca taatcgctta taaggccgca tcaacagcta ttactaatgc gcgcctgaat
    29461 tatatatgat atgaaggagg ggctaaaagc ctctccacaa taaacaaaat tatttagcag
    29521 cttttggttt cttgacaccg tagcgactgc gcccctggcg gcgagcctcg acgccagcag
    29581 catcataaac gccgcggaca attttatagc gaacaccagg aagatcctta gtcttaccac
    29641 ccttaatcaa aacaatggag tgctcttgca aattatgccc cataccagga atataagcag
    29701 ttacttccat gccgttggac aaacggacgc gagcgatttt acgcaaagcg gaattcggct
    29761 tcttgggggt agttgtagtc actttaacac aaacaccttg cttaaaggga gcgcctttga
    29821 catttacatt ttttttgcga tgcaaagaat ccaggctggt atgcaaaact aaagatgatt
    29881 ggcgctgact tttggctttt ctgcccttgc ggactaattg gttaattgtc ggcataatgc
    29941 ttcaacggtt ttaaaaacct tcgttataaa aatgggttga aacccataaa atgaggagca
    30001 aactaaaatt gtcgctctct cttttgatta aataaaattt ttgtgataac aaaatatcct
    30061 gacccatcag gatagagtta ttactcgcct actttatcaa aatcaaaaaa ataagtcaag
    30121 ataagcgaag aatttgattc tcttggccat ctaccagaac gagattaggc ccctgccaac
    30181 aaacggaaaa gccagtcaat cagcggcaga agatagggaa agcctatcca agcgaataaa
    30241 atcacaaaaa tgaaaccgaa tctttctaaa ttgagatatt ttaactgtgc tgagggcgag
    30301 aggaagggcg ccaatatttt ggaaccgtcc agcggcggaa tcggcaaaag gttaaaaatc
    30361 atcaaaatga gattaatata gataattaaa gcgattaaac cagaaaaagt cagactgata
    30421 aaaggtaaaa gccgcaaaag aagaccaaaa gcaagggcta caataaaatt agccgccggc
    30481 ccggccaagg cgactttagc cccgccccag cgacggtctc taagattata gggattatag
    30541 ggcaccggct ttgcccagcc aaaaacaaaa ggcattcctg agagcaacat caaaccggga
    30601 agcaagaccg aaccaaacca ttccaggtga accaaaggat ttaaggttaa acgtccggcc
    30661 agacgggcag tattatcacc cagacgatcg gccatccagc cgtgcatgta ctcatgaata
    30721 atagcagaaa agattaaaac tataatttga aataaagcga gcataaaaga aaaacttaaa
    30781 ctatccctaa ttaattatga ctttatttta gcacaacttg attaatttta aaaatgtgct
    30841 aatatttaag catcaaaatt ttaaatatag cggccgccgc caaaagccag ttttttggtg
    30901 tctttctctc ggccgtttcc ttaagcatat ggctaaaaga tgtgatttat gtaaacgcgg
    30961 cgcgactaaa ggcgccagcc gctcccactc caaaattcaa accttgaaac gccagggcat
    31021 taatcttcag actaaaaata tcgacggttt aaaattaaaa atttgcacta gctgcctgcg
    31081 gacaatggct aaaaacaaca agtaaacaat cactgaaaaa gaaaagggac tcctcaagag
    31141 tcctttttcg ttggccccaa aatcaaccgg cggcgcttag ttaaaatata ataacgccca
    31201 agaaaaaata atattatgac aaaataataa gcgataacat aagccaaatg aaaattaggc
    31261 gtttccacat aagctccagg aatagacgcc gccaagcgcg ccaaaaacaa aagataatta
    31321 agtagcgctt ttgacggtag ccaaataaat tcagaaaaac caaataagaa attaaaaccc
    31381 aacgatgaaa tcaggctaat cattagaggg ctgaagacac tgaaagctaa aatattactc
    31441 aacggagcaa ttaaagaaac acccccgctc cgccaagcca aaagcggcca aatggttaat
    31501 tgcgccgaca aagataaact aaaagcttca aaaacaagac gcagacgact atgggtaaag
    31561 tggtgggtga aaacagatgt taataaagga taaaaagcaa tcattccggc gattgccaaa
    31621 aaagataact ggaaacctaa atcatgccgc cataaattag gattattcca caacataatg
    31681 gcacccgcta ataataaagg gagccaagcc gaacttaagc gtcctcggcg ccaagcatac
    31741 aaaaaaatgc ctcccatcag taatgaacgg aaagccgaag cttgccagcc ggtcaaaaaa
    31801 acatacaggc ccgccccaaa taaagccggc cacagggcaa agcgcttatt tatccccaaa
    31861 taaaccaaca gattaatcag caagcttaaa aatagggtga tatgcccccc ggaaatggcc
    31921 accaagtggc tcaaaccggc tttctgccaa attaaatttt ctgccggaaa taaagttttc
    31981 ttgtatccaa gcaataaagc cgccgccaat ccggccgctg gttccggcaa agattgattg
    32041 atttgccgca gcatctctct tttaaaaata aaaaggcgac taaaaatatc gcggcggttt
    32101 tcctctcttt ctaaagcatt aacttgaggc caagcacaaa gagcgccgat gccgcgcgcc
    32161 gccaaaaaag ccgcataatc aaaatcagca aagaccggcg gtttttccaa aagacaatgc
    32221 aaatttaaag tatcgccata ataaacacgc ggatataaag gcaggttagc gataattttg
    32281 tctttgctaa attttccaga agaaggctcc aacatgcaca aaattacaat ctgcttatcc
    32341 caagctggct cagggtcggc acatacttga aactgccaat ttgatgcagg cttgcctaaa
    32401 taaggagtta acaacgaatc agccgccgcc gaaacgcttg cccgccaagc gccgagacaa
    32461 aaaacggcta gtaacataaa aaacaaacgc ccgcgacggc ttaaaaacgc taacccaatc
    32521 gcagcaaaaa atatcaaaat aattggcggc caagcctgga aagaagcaac ggctaaaccg
    32581 gcaaaaaagg caaatagata taaaaaaata gaagcggaca tgcttctatt ataaataatt
    32641 aaaataaccg gtccgtaaaa tttatctaaa caaggtcttt tcgccaacgc agataagcta
    32701 tcacaaattc atcaagatgc ccgtccatca cgccgtccac atccgtgtct tcccaattac
    32761 tacggtgatc ttttactaaa cgattaccat aaaaccaata ggaacgaatc tgttgtcccc
    32821 actccgcctt gaccacgcca ccctttaaac gtttctcttc cgcttctttt ttctctaatt
    32881 ctaatttagc caaacgcgcg cgaagaatgg agaaagcgtt atctttattc tgatgttgag
    32941 agcgttccgt ctgacaacta accgtaatcc ctgtcggtaa atggaccagc ctgaccgccg
    33001 agtcggttgt attaacgctc tgcccgcccg gaccgctaga ccgaaaaacg tctaggcgca
    33061 catcttcgtc tttaatcacg agatcgttag gcagtatttc gggaatgact tccactgagg
    33121 caaaagaggt ttgacgtaaa ccatcggcgt taaatggaga attacgcaag agccggtgcg
    33181 tcccgttttc acttcttaaa ttgccataaa ctcttactcc cgtaattttt aacatcacgc
    33241 ttttaattcc cgcttcattg gctaaaaggc gatctataat ctcggttttc cagccttgtc
    33301 tttcggcgaa gcgtaaatac atccgctcca gcatctgcgc ccaatcctga gcatccactc
    33361 cgccggtgcc agcattaaca cttaataaag cgttactctc gtcataggcg ccggtataaa
    33421 gagacagctt ctctagttct gctaaatcgg ctgatagctt taaagattga tcagatagtt
    33481 ccgccaaaag tttattatct ttctcactgg ttgccatttg cgccaactcc ttagtctccg
    33541 ctagcgactg ttcaagactt agccagcgtt gataattttt ttgcaaattt tccgcccgcc
    33601 gattaacaat aactgccctt tcctggtctt gccaaaaatc cggcgcttcc ctttgttggc
    33661 gtaaaacctc aatttctgtt tttaattcat caatcctaaa aactgcagcc aattcttgtc
    33721 gccgccggtc taagtcttct aaaaatttac ctagattatc catacattag ttacatcaaa
    33781 aaataataat ccaaaggaat aaaaaccgtc tttaattcct cgttgatttt taattcggta
    33841 tttgaaaaaa tcaaattata atcagaatta atttttttag tggtagtggc tatttgtttt
    33901 acatctttat tccccatgcc aacttcaatc actatctgtt tattgttcat aatctgcaaa
    33961 ataaaatcag ctccgccttg agcgctgtca tatcgcacca aaccctgatt ttttaaaata
    34021 aattcacgat aaagatgaga tgcaaaagag tcttccagca acttgccttg tctggttaaa
    34081 tatgtgcttt cttgccccgt aaaataaaag aaagtcattc tgattgccgg cgacataaac
    34141 aaatatttat tcggcttctt ggcaactgtc atatttgatc cataagccgg cacttttatc
    34201 aataattcca ctttttccag cgcctcaaaa atatttgata tagttaaacg atttattttg
    34261 aatctctctt ccaatgtgcc gagactggtt gtgtcgtttt ctgcgatagc aaacaatatc
    34321 ctttttacag cgcccaaagt atcttgatca aaacttccaa gagttggtaa atcttgtttt
    34381 ataattttat ccaacagtaa tgatatggca tcatacaccg ctgtttcgtt tggcattata
    34441 agcgaaaaag gcaaggttcc gtatgataaa taagttctaa tatcatgtct atttattttt
    34501 gaccaacaat tattaacatc tccttgtaag tcctgcaacc tattaaacac ttcttctgca
    34561 gaatttgaaa aatatactat ttggcgtatc ttatctttta aaccgttttt aggaaatgtt
    34621 tgatttttaa ttatctcata ctctataaat gacatgggcg gcattctctc aaataaagct
    34681 cttctaacca aatttgttgt agtttgtaaa ataattgccg atgagccagt acaacaaaaa
    34741 aatacatttg atgttttgtc aaataaagtt ttcagactaa tagcccattt agggtccgtt
    34801 tgtatttcat ctaaaaatat taaagttggt ttttttagtt cttcaatact ttgcccaata
    34861 acattttcaa aagcaaaaat aatatcattg actcccgcgt ttaataaatt ttctgtttct
    34921 tccacggata aaaataaaat attttccacc ttccttgaat aagctgcgca tatttgcgcc
    34981 attaatgtag ttttaccaac gcccctaaaa ccaggaataa taaccattct gttatcttga
    35041 gttttatcca aaaatccatc aatgtactgt ttgatctttg caaacataaa acgttgagga
    35101 tatttttctc ctctggggct gtgtgtatat ctcttaattc tttctctcgt ggttctaatc
    35161 tgctgttgta tatattgtat caaatcatta ttcatgttta ttatgttata ttacaaatgc
    35221 ctatctctag tatattataa tatacaagaa gcgtcaaggc ttgattattc agctaaaatt
    35281 aagcataata tcacctattt acttgtttta acatttaata tataactcca tttttttgtt
    35341 attattgaat atttctggat tcccgccttc gcgggaatga cgggtgatta gcaatattac
    35401 ggagcgagaa aatcacctct tttctgaaat ttcgaccata catgattaaa ttaaatatca
    35461 aaaaactccc taaaattagg aagttttttg tatataatct atagagatat ctaagagata
    35521 tctatgttaa cgcttgctcc actgcggacg tttgcgcgcc cccttaaaac caggcttctt
    35581 tctttccttt ttacgcggat cgcgagtcaa aaatttattg gctttcaaag cttctttaag
    35641 tagtgggtcg agcaccagta aagctcgggc gaggccgtga cgacaagctt caatctggcc
    35701 ggtaataccg ccgcctttca cgataatgga aaaattaaga tcacggacat ggccggtagc
    35761 ttttaatggc tgagtaacga tactcatgcc ttcaccgaca aaaaattggc tgcccttctt
    35821 gccgttaatc ataataactc ctttaccgtc tttatacatc ctcacttgag caacggaagt
    35881 cttgcgacga ccaacagttt gaatatattc ccccttaaac tccgctgttt ccggtatatc
    35941 tttaaaagca tttttcttcg cttcttcttc attagaaaat tcagtcgaca cctcttcaat
    36001 caccgccttc ttcggcgccc ggactttctt ttcttttact gttttagtct tcttttccat
    36061 aaaacgttag attatctaat aattaagcgc ttcatttgcg cgtcgcgcaa tttggtcggg
    36121 ggtaacatct gttttaccgc ccggcgcaaa acttctcccg gattctgact gaaaacctca
    36181 gacattcttt tgctcttcaa accactgaga taaccggaat aactaaaata tttcttctgc
    36241 aggagtttct ggccgctaaa agacatttgt gcaatatttt caacttcaac aatatcgccg
    36301 ccatcaacat aaggcagaaa ttccggctta tttttgccgc gcaaaataat cgcgatctga
    36361 ctagctaaac gtccgaccgc ctggccggca gcatcgagtt tgtggatttt tctttcaatt
    36421 ttattcatat tggtgtttac gcgtaaatta aacaagttca atctgaacca tttctgcgcc
    36481 gtctcccgga cgcggaccaa gtttggttat acgggtataa ccaccaggat gattctgata
    36541 acgctggccc aaaacttcca ttgatttttt cactgcattt ttctgtaata aaactttaat
    36601 aagtccgcga cgggcgttta aatcaccggc tttagcggcc gtaatcattc tttcaaccaa
    36661 aggacgaacg gcttgagcct tcgctttagt ggtcttcact ttttcgtaaa tcaaaatgct
    36721 ggaggcaaga tttcggagca ttaactcgcg tggtcccttt ttccgatcta gaattttatt
    36781 agtgtttctg tgacgcatat attaaggata tttataaagg cttatttttt gctttttttc
    36841 ttggctggct tgtccgcttc gtcggcgtca acttcagcca cttctttagt ctctaaatca
    36901 acagactctt ccttggactc aatttccggg gccggttttt gagctgaagg taacaacgcg
    36961 ctgaactgat cgactaaaat ttgcgcggct tgattaaaag cttccctggg actgatggtg
    37021 ccgtctgttt tgagggtaat taaaagttta tcccaattag tcattttacc aacacgagta
    37081 ttttccacat ccaaactgac cgctaaaacc ggagagaaaa tcgaatctat ttccatataa
    37141 cctaattcat tattttcttt cttggttccc tcgcttaaac gataaccgcg gccaggacga
    37201 acaaagattt ccgcttctaa agaagctttt tcttcggtca aagaggcgat taatagttcc
    37261 ggatttttga tttccacctg gctattctta gtgatattag cggcggtcac cagctgctta
    37321 cctttaactt ttagttccag gcggacctcc tcgtcgccgt acattttgat atggagctgc
    37381 ttaatattaa gcattatttc caaaacatct tcctgaatac caggtaaagt catgaattca
    37441 tggctggcgc ctttaatctt aacgccgact accgctgctc ccggcaaaga agacagcaag
    37501 acgcggcgca aagaattacc taaagtcatg ccgtaaccgg gatatagcgg ttccacgaca
    37561 accactccct cattctcggt ttttcctgcc aagaaatcta ctttttttgg caaagcgata
    37621 ttttccataa aacatatgcc ctcagaacgc agttttacgc ttcctaatta gcgagaagcg
    37681 cctcctcagc gatccgattg acagattatt ttgaataata ctcaataatc atctgcacct
    37741 taaaatttgt cggtaaatca gtgtcggccg gttcatgtaa aaccttggcc ttcatttcgg
    37801 cggcctgtaa atttagccaa gccggcaact ccaccttttt taaactctcg gcaatttgtt
    37861 taaaatatgg gctttttttt gcgcgagcac gaacggcgat ttcctgtcca ggcttaacta
    37921 taaaagaggg gatatcagtc ttcacgccat taacctcaaa atgtccatga tttaccaatt
    37981 gacgggcttg actgcgagat ttagccagac cgagacggaa aacaacatta tctaaacgca
    38041 tctccaataa acgtaagaga tttttaccgg aatcgccagt cttctttgag gctttctcaa
    38101 aagttaagcg gagctgtttt tccatgacat tataatattt cttcgctttc tgtttttctg
    38161 ctaactgagt accataatca ctgagtttgt ggcgaccgcg agagggcccg tgaaaaccgg
    38221 gggggtagtt acgcttaacc atggcgcatt tcggcgtatt acagcgttcc ccttttaaaa
    38281 acagcttctc accgacacgg cgacattgct gacatttact gtctaaattt cttcccatat
    38341 gatataaatc tttaaattaa ttaaactcgg cggggcttct tcggacgaca gccattatgg
    38401 ggaacggcgg ttacatcttt aatagccgaa acctccaaac cattagcatt taaagcgcgg
    38461 atagccgctt ctctgccggt accgacgccg gcaacaaaaa cgctgacttc ctgcagacca
    38521 tattcttctt tagctttctg aacagcaatc ttagtaataa tctgcgcggc gtaaggagtt
    38581 gctttcttcg cccccttaaa accagccata ccggcgcttg cccaggcaat aacatcacca
    38641 ttagagtcgg tcaaggtgac catagtgttg ttataagtgg cattaatata ggcgcgaccg
    38701 actttaaccg tacgagctgt gcgcttcttg cgcttcttca ctaatttctt tttagccctg
    38761 gcctgcttat ttttttctaa tttctgttta atttcttctg gcaattcttc atcagcgcca
    38821 gaatccaaag aaaaatccgg gctttcatca cgatcgtctt cagattcttc cgccgctgct
    38881 ttcgcttcgg ctggcttggc ggtcgctgct ggcgcctcta tcttttcttc ttcagaattt
    38941 aaattctttt cttcaggcat aataattata aattaatctt gagaaaacga ctgatgacta
    39001 agtctttaga ccggccttaa cgcgaccact ggtagccgtc atgcgcttat tgccgcgcac
    39061 ggtgcgactg ttagtcttag tgcgttgacc acgaaccggt aaattgcgac tgtggcgctg
    39121 accgcgatag cagccaattt ctttcaaacg cttaatattg ctcgccactt cacgacgtaa
    39181 atcaccttca actatatact tcttctcaat ttgttcacgc aaacgattgg cgtcattttc
    39241 tttcaagtcc ctaactttag tctcaggctt gatatttaaa gcggccagga ttttttttgc
    39301 ccggctgttg ccgataccgt agatataggt aagggcaata tctactcttt tgtcattggg
    39361 aatgtttacc cccgcgattc taatagccat aaataaatat tcagccgcgc cgcaagagct
    39421 tggcctaatg cttaaattat ccttgtcttt gcttatgttt aggattctta cagattacat
    39481 aaacgcgact cttgcgacgg acagtcttgc aatctttaca aattttctta acgctcgctc
    39541 ttactttcat aaaatcatga aaaatataaa aagggactaa atccacttaa aaaattaata
    39601 gcggaaaata atacgaccct tagttaaatc ataggggctc atctgtaaat gaacccgatc
    39661 acccggcaat aatctgatgc gatgcattct catcttaccg gacaaatgag ccaaaatttc
    39721 atgcccacta tccaatttta ctcggaaagt cgccgccggc atcaattcta atacttctcc
    39781 ctgcatctct aaaaaatcct tggcattcaa agtctcgacg ttctcttggt tgtttgtttt
    39841 actttttgac attaacacaa aaaatttgat aaagacaatt tttagcttta tcaaaatttt
    39901 ttagaaatat tcctcttaga aatctcaatg gaaatagtat atagacaagc ttaaaaattg
    39961 tcaatagtaa cgggcatccg atctgatcat tctaaaatag ccttaatcct cctgacaccg
    40021 gacgacgagg cctcttcttt ttgaatctta aaatgtccga gatttttaag actggaaaca
    40081 tggggaccgc cgcaaatttc tctggaaaaa atatcttccc aagacgcgtg tctgccgcca
    40141 ttattatgcc caatggtata tactttaacg cggtcaccgt atttagaatt aaaaactccg
    40201 gtcgcgccgc tcgccctagc ttcagataaa gaaatctcct caaaagcaac gtctaaaccc
    40261 ttaccaatcg cctcgttaac caatttttca acagacgcaa tttgtgccgg cgtcattttt
    40321 tccggataag aaaaatcaaa gcgcagacgt tcggcggtga tattacttcc cttttggcta
    40381 acatgattgc ccaaaacttg gcgcaaggcc gctaagagta aatgagcggc cgtatgaagt
    40441 tttgttgttt cttcgctgtg gtcagccaaa ccgcctttaa atttaccggc agaagcggtg
    40501 cgggaaagtt cttgatgctt ttgtagttcg gccataaaac cctgtctatc aaccttaatc
    40561 ccgtgttcag cggctaattc ttccgttatt tctaaaggga aaccgtaact ttggtaaaga
    40621 ttaaaagcgg ccacaccgtt taaatcatgc gtcgctaatt ttttaaattc aatcatcccc
    40681 ttctccagag tccggttgaa tttttcttct tccttttcaa actccgcaaa aataaattct
    40741 tgattattct ttaattccgg ataagtggcg gcgtagtcat caataaaaat gagagccgct
    40801 tctttcaacc acttctcctg agaaataccg atttgacgac cataacgaat agcgcgacgc
    40861 agaagacggc ggacgatata gccctgatca gtatttgagg gcggcacgcc gtggtcatcg
    40921 ccgacaataa aggtcgccgc ttttaaatga tcaacaataa cctcaaaagc cctcttatta
    40981 tcagcatatt tcttgccgga cagttcttca attctcgcca tcgccctggt aaaaagatca
    41041 gttttaaaaa tactgtcttg tccgtttaaa accattaaaa ttctctccag accgccgcca
    41101 aaatcaacat ttttattctt taattcctgc cagccctcag ctgttttctg atattgcata
    41161 aacaccgaat tgccgatttc caagaaacgg ccgcaatcgc aattaggatg gcaatgggga
    41221 ccataagcta aatcatgctc tttgccggta tcataaaaag tctcactgtc cggtccgccc
    41281 aactcaccta cgacatcgcc cttttgccac caattcttat cgcgataagc aaatattctc
    41341 tgcctagaaa aatcaagctc ctgaccagaa ccgaggacgc ctttaccagt agtaaccggc
    41401 ccttcctccg cactaacacc atatttttta aacaaatcct gtaaaatcaa aattgattct
    41461 ttgtccttgg ctatttttcc gtcttcacta ccagcataaa ctgtttgata aatacgagat
    41521 ggatccaatt ttaattcaac aaataaaaac tctagccacc aggctaattg ctccgctttg
    41581 aaataatcac ctaaagacca attaccaagc atctcaaaga aagtattatg gcgaccatcg
    41641 ccaacttctt caatatcctc ggtgcgaaaa gatttttggc tattaaccaa acggatgccg
    41701 gccggatgct tttctcccaa aagataaggc actaagggaa acatgccgga gttggtaaat
    41761 aataaagtag aatcattttc cggcaataaa gaagcactgg ggatgatggc atgacccctg
    41821 gccttaaaaa aatctaaata tttttgtctc aactcattag cggtcatata ttttaaatta
    41881 aaccctaaaa attaatcttt tattattttc ctataagctc taaatcgcca ttaatgatag
    41941 ctagcgaaat gtctgtcaat tcatcattat aattaccgcc gccggtcttt ttctcaggga
    42001 aagtaccgtc gccctgttct gtctttttct taaaaaattc ctcgtattaa ataatattaa
    42061 cttccggttc aatctttatc ccgaatttct gataaacgcc agcttggacg cgacggacgt
    42121 gcgccaaaac ctgcttggca ctggcgccgc cataattaac aagaatcaaa gcctgcttct
    42181 catacatgcc gaccggcccg taacgcttgc ctttaaagcc cgcctgctca atcaaccaac
    42241 cggccgaaat tttaactaaa ccgcttttag agccggggaa gaattttatt tctggaaatt
    42301 tcttctgcaa acccacaaag tcagctttgg tgacttccgg attcttaaaa aaggagccgg
    42361 cattaggata aatggcgggg ttgggtaatt tattgtcacg aatctttctg ataatttccg
    42421 ctaattgctg aacgctcggc cgcttaattc cctgttcgtt caattgggcg cggatatcgc
    42481 cataatccaa acgcaaaacc ggtttcttcg acaacttcaa agtgacgctg gcaataaaat
    42541 atttgccttt ttctttattc ttaaaaacgc tagagcgata gccgaaagcg caatcttttt
    42601 tagtaaaaat tttttccttg ccggttttta aattaattgc gcgcaaactc acaaaagtat
    42661 ctttgagctc aacgccgtaa gcgccgatat tctgtaccgg cgccgcgccc acggtgccgg
    42721 gaacataata taaattttca attccgcccc aacggcgctc aatcgcaaag cgcaccaaag
    42781 tgctccacca ttctcccgag cgcgcttcta cccaaacgga tgtttccgtt tccttaatta
    42841 cccgtacgcc cttaatttca ttttttaaaa ctaaagcctt gatcttttgc gtgaagagaa
    42901 tattggaccc gccgcccaga ataaatactg gtaatttctg aaggcgcgcc caactcaaag
    42961 cttcctgcaa ttcagtctct tcctttataa cagccaaaaa ggccgcctta gctttaatct
    43021 ttaaagtggt ataagcagaa atatctactt gtttttggag tgtaaacata aaggtattat
    43081 aacgcaagta taggaaaaaa gtctagacaa ggggcaataa aaagcccacg ttttttaggc
    43141 gcgggtgaat aaagcgggag agaagcaaga gattagtatc acctacttag gcaatcaccg
    43201 gcaaatgttc taaacttaat agctttctcg ccggggtgag aagcgatcta agaccatttt
    43261 caagtaaaaa tacccgaacc ggttggtctt tattatctat gaataagggc taacaaggct
    43321 ggtcattgac aacagcgtca gcggccacaa ttttattctg atatctctcc caaatcctgc
    43381 aacttcaaga gcttagcata tagacccccg gctacttggg acaactctaa atgactgccc
    43441 tgctccgcca ccttgccgtt ttctaaaaca ataattttat ccgccttctt tatcgtactg
    43501 aggcgatgag caatgataat tactgtccga tggcggccga tgcgccctag agcttcttga
    43561 attaaagact cgctctggct atcaagatta gaagtggcct cgtcaaaaat taagatttta
    43621 ggacgggcga gaatagctcg ggcaataccc aaacgctggc gctgaccacc ggataattta
    43681 ataccacgct cacctactag agtgtcatag cccttatcca acttcaaaat aaattcctcg
    43741 gcattggcaa tacgagccgc cgcctctatt tcggccaaag aggccgccgg attagcgtaa
    43801 gcgatattat cgcgcacgct cacgtcaaat atttccactt cttgcgggac aatcgctaag
    43861 aagcgacgat aggcgttaaa atcaaatgat tttatatcct tgtcgtccac catcactttt
    43921 cccgaagtgg gatcgtaatg tcgataaagc aaacgagcaa cggtagtctt gccgccacca
    43981 gaaggaccga ctaaagccac cgtggtaccg gcgctaattt taaaactaac cctatcaaga
    44041 gccatcactt tagcctcacg atattggaaa gaaacatctt taaactcaat cttgccttca
    44101 attttctcag gaattatcgc accctcttca ttcttcacct cgctctcggc attaaatagg
    44161 gttaaaaggc gattaacccc tgcccgtcct tcttccatgc ggtcatagaa acgagaaaga
    44221 cggtaaatag aagaataggc catctcgctg agagtaaaag caaaaatcaa agtaccaacg
    44281 ctgatttcgc cgtgatagac aaaatacaca cccagaaaca aaaccgccgc ccgaccgata
    44341 tcaacaatga tattgcggcc tagaccaatc ttcatcatcc agccccactg cttattttca
    44401 ttatcgcgaa taccggcctt gatgtcgcta aattcattaa actccctttc ttcctgaaca
    44461 aaagattgga cagcattgat attaataata gattgcgcca ttttgccgga agcagtttca
    44521 taagatttat atatttcctt tctcaccgga tacattttct ggttcgacca ataagtgaca
    44581 aaaataaata aaggggtaaa aatcaaaaat gatataccga tgcgccagtc cgcccaaaat
    44641 aaggccggga tggtaaaggc tagttgtact aaagtcggcg ccacttccca gctaatatta
    44701 ttaattagcg tcgaaatccg atcgacccct ctctcgattt tgacgatttt ggcaccggta
    44761 ttctcgcgtt cgtgatagcc caaacttaaa tttaacagct tatcctgcgc ttttaaacct
    44821 aaatgatatt cgattttaac taataatttc aaaatagccc gatcattaaa atactggata
    44881 agggaacgaa cctgaccaga accgagatac aaaacaatca gccacaatag aaaagataaa
    44941 tcgattgatt gaaaattata taaattatca attaaaatct ttaaaatata gggtttagcc
    45001 aaatcaaaaa ccgcccccaa gacggtgatg gctaaaatgt agttgattaa gcggtggtcg
    45061 ggcgataata ggcgccacaa ctcgcgccag aataaacgcc aacttaggct tggctccatc
    45121 tctttagttt tgtccgaatt ttcgttcata aaaaatttaa aaacggattg atccgctctc
    45181 tatagaatac ggaaatatct aaaaaacatt acattttcct aaaaataata aataagttag
    45241 gttacgtact agcttattac ccggaagtac gttcgttcac ccacttattg acaaaaagta
    45301 aattatttac taaaactaag acagttcact caccttttaa aaacataaaa atcatggaaa
    45361 caactcagat tacagaaatt tccgtccttg gcaaaaagtg gaaaaccgct acaagtggag
    45421 aaaaaaaaga aatatcaaaa aaaatctccg cctacctgct taaagcagat gcccaacaat
    45481 taaccaccct acttcccagc attaaatctc tatcctggct taccgatgaa tgtctggatt
    45541 atgtcctggg aaaaatatat gacactgata aaataaaaaa actttttcca ctgagtagga
    45601 aaaaaacggg acaacgacaa aaattggccg aaagatacgg cgcccttata gaggccaaat
    45661 ttcaatcagc tcccttcaaa gaaaaggtgg caattttcaa agaagccaat aatcaggagg
    45721 gatttaccga aattgaccaa gaaaacaaga aattttgcca gcgtcactgg caagatttca
    45781 tccacacaat caataattat gaaaaaggtc tattgatata tgatgctttt atcggcaacc
    45841 atcttacctg tcccgaaatt tgggcaacgg tcgcagccat taaaaccatt aaggtgtgga
    45901 ccaaacaaaa caaccttaaa aagatcgact ctggacaggc ggctttgcta caaaaattaa
    45961 accttagcct gcagccagga aaaatcctcg ttgctcagga aattctcaaa aaaatggggc
    46021 aataaaaaat atgataatat gatacttttc ctaaaaaccc gaccacttgg ccgggtttat
    46081 ctttataatt cccatcaaaa tcatcagctt ttctttttta gcaatctctc tcctactcgg
    46141 aaaatatcac ccgcgccgat aagcaacaga atgtcattag gctttaaaat tttcttcagc
    46201 caagacaaag cttggtcaaa atcagacaga taaacggccg gctttttcct cccggccgct
    46261 ttaagggctt taactaaatc ccgactggaa acacctccct ggacttctct ggcagaacca
    46321 tatatttcca atatccccaa cacatcagcc tccgcaaaac tatgaataaa atcttggaac
    46381 aaagcttggg tgcgggtaaa agtatggggg tgaaaaacaa ccaccaaacg cttgcccgaa
    46441 taagcttcct taaaagcttg aagagtcgct cgcacttctg tgggatgatg tccgtaatcg
    46501 tcaaatactg gtattttacc gtacttgccc attagctccg cccgccgcgc ggccccggaa
    46561 aaagaggcta aagcggtctt taattttcta atatctaatc ccaaagccca agcggtggct
    46621 aacaccgcca gagcattctc aacattatgc tcgcccaaaa gcttgatttt aaattcaccg
    46681 aaatcattca cttgaaagta ctgataacca tgacgccggc gcacggaatg agccaacagg
    46741 gtgatatttt gctggcgccg ctgcactttg tcagccagcg cgtaggtaaa aacctgcgcc
    46801 cgacagagag gcaacaactt cattatttgt gcgtcagccg cattagccgt taaaaaaccg
    46861 gaaggaggca actttttgat aaagtcggca aagacgcggg cataagcggc tttagtctta
    46921 aaataatcag ggtgatcgta gtcgatatta tttaaaacga tgccgtgggg ctgaaaatat
    46981 tgcaacttat tctgatactc gtccgcttcg gcgataaaat aatcggactt gcctaaaatg
    47041 gcgctgccct taaactgcgg caccggcgca cccaccagaa cattaggtgc cagaccggct
    47101 ttttgtaaaa cgaaaccaag ccaggcggcg gtcgtcgtct taccatggct gccgcaaacg
    47161 gcaattccca aataagaact gaaaagagcg ccaattgcct gagcataatt caaaatcggt
    47221 aattttttaa atttatctgg attatttttc aaataagcca tctctatgtt gttgtcagta
    47281 gtataggcgc tggaataaat aatgaccgcg tctgtcggaa tttttgaaac atcaaaacca
    47341 agccgaacag aaattttttc tcgacgtaga actttatcgg tcataaaaac atcggcgata
    47401 tcaaccccgg aaaccgcttt cccctgctct tttaaaaact gcgccagcat tgtcatccct
    47461 acgcccttga tgcccaacaa ataaaatttt ttatatttca ataaatccat atcaagtatt
    47521 tattagatca tacataagtg ccgccccttt atcagcggcg tcggccggca acaaatgact
    47581 gatatttttt gataaaagcc ggcgtctttc attatcctgc cacaaagatt tcaaggcgtt
    47641 agctaaagat tcagcgctgg tcgtttcttg gtttaaaacc aaagcggcat tattatcggc
    47701 aaaataggca gcattatctt cctgatgaga atgaggtatc ggtataagca aacagggctt
    47761 ggctaaaaga gacaactccg ttaaagcccc caagccggcg cgcgacacca ctatatcggc
    47821 caaagacatc agcacttgat aatcctcatg gctaatactc tccaccaccc tataacccgc
    47881 ccgcacgggt gcctcctccc ctttgccagg accggtctga tgaacaatct gccagccggc
    47941 cggcaaaagt ttaactgtgg agaaaaaaag acgatttaga gccaaggcgc caccaccacc
    48001 gccagttact aaaattaaag gagccgtatt gttcaattta aaatttacca gcactctttc
    48061 cttgtcgtta cttgagattt cagcgaccaa actaggattg ccgattaacg tcgccctttg
    48121 accataatcg gccaaagact tagaaaaagt gacgcttaaa caatcagccg ctcggctcat
    48181 taaacgattc gccaagcccg ggcgcaaatc ttgctgatga ataaaaatct tggcgccgtt
    48241 taactttccc gcccaaacca ccggcacgct ggcgaaagaa ccggcggaga caatcaagtc
    48301 gggagacaaa cgcctaaaca aaaaataggc tttaaaaaaa ccggaaataa ttttaaaaat
    48361 atcgaaaaaa ttggaccaag aaaaataccg ccgccattta ccggccggta aagcataata
    48421 atcagcccct aattcctcaa aaattcgacc gaataatttt ttttccaatt cggggctgtt
    48481 gcccaaaaaa ataaaacggg cggtagcatc caaacggcga taagctaccg ctaaggctaa
    48541 aggagctgta ctgggaccgc ccgtaccgcc accggaaata acaattaatt ttgtcttaag
    48601 agcgtccata tttcgtgtgt ttactgatgt tagctaagag gccgattgcc attaaggttg
    48661 ctaaaatcga agagccacca gcggagataa aaggtaaagg cacacccgtc atcggaataa
    48721 gattaatcat gccgccgata tttaaaaatg tttgtaccgc taaccaagcc ataatgccgg
    48781 tagccaaagc gctgccataa atatcggggg catagcgggc aatcttaaac ccgcgataaa
    48841 ataacaggcc gtaaagaaaa ataagaaaag tgctgaagac gaagccgacc tcttcagcaa
    48901 taatcgaaaa aatggaatcc ccccaaactt ccggcaaata cataaatttc tgtcgactct
    48961 gccctaagcc ccgccccagc cagccaccgg aaccgacggc aattaaagat tggttaattt
    49021 gataacattc tttctgagaa tcataggacg gatcttgata acatttaaag cgatcagttt
    49081 tataggaaga accggcgtaa agtaaaagcg ccaacgaaaa agcgcccaag actacagtta
    49141 gaacgatgtg ggaaaattta ccgcccgcca caaaaaaagc ggcgaaagcg gcaaataaaa
    49201 taataaaaag agtacctatg tccggctgag ccatcattaa agcgctaata actaccagac
    49261 tgcttaaaaa agggatgatg ccgctggtcg ctttattcaa ttgccccttt ttagcttcaa
    49321 gccaagtagc cagataaatc aaaaaactca gcttcacaaa ctccgagggc tgcaaagact
    49381 gtctgaaaat tgttatccaa ctatgcgccg tcccgtattc agatctcaaa ccaggaataa
    49441 aaaccaaaac tagcagaaaa atagaaataa acaaagccca gacggcatag cgtcgccaca
    49501 gacgataatc gattttatag aacccccaaa aagcaccaag acctaaagtg acgaaaaata
    49561 attgcctaaa aaaataatga taagtattcc catatttaac ataagacacg accgaagagg
    49621 cgctggaaag cattactaaa ccgataatca gcaaaacgcc cgcggctatc aaaaaccagc
    49681 ggtcaggctg atgttcctga ctgctaggag caaaaaaatt tctaaggctt ttccctatct
    49741 tcatagaaaa aattgccatt gcctgtcaac aaaaaatatt atcagaccaa ggaccgcccc
    49801 taaagcagca attacccaga aacgcatcac cactttagat tcgggccagc cgatagcctg
    49861 ataatgatga tgaatgggcg aagaaataaa aactttttta tggcggcaac gcttagaaat
    49921 ggtctgaata ataaccgaaa gagattcaat taaaaaaata aaaccgataa aaggcaacaa
    49981 taaagcgctg ttagtcagca tcgcgataac acctaaagtc acccctaaac tcatggcgcc
    50041 cgtgtccccc atataaaaac gcgccggcgg aatgttaaac cataaaaaag ccaacaaggc
    50101 gccgataata accccacaaa aagccgctaa ttcataacgg ccgagagcaa aagaaataat
    50161 gccataagcg ataaaagcaa tcattaaagt gccgccggcc aaaccgtcta aaccatccgt
    50221 ttcattaacg gaaaaagagg tggcgacgat aataaagata aataccggga tataccacca
    50281 agatatttcc aagtttccaa agaaaggaat atgaaaaacc gtccagtcta atttaaaata
    50341 aaaccaagca gcgcccgccg cggcaattaa agtgtatatt aataaacgat gctttaactt
    50401 caagccgccg ccgccgaaaa cgccacgacc gcttacatcc agccaatcat ccaccaagcc
    50461 gataatggcg ctggctacta aaactcccag gggtaataat gtttccgaac gggaaaaaaa
    50521 attcaaatat ttaaaaatct cgcccggcat cacgtcagcc aaaaaagaaa acagaaccat
    50581 aaagagtaag actgtgcccc agattaacac cccgcccatg gtgggggtac cggctttatg
    50641 agcatgtagc ttactaaaaa caggcgtttg cccgccgttt ctaattttct tccctaattt
    50701 gtatttatat aaaaaattag tcaaagtagg ggttaccgcc accgcaaaaa taaaagccaa
    50761 agtggaaagt aaaagaattt tgacaatata aaaaatggtc atatttaagc gagaaaatta
    50821 atcaaatata aagacatcag cgccaaagcg gccaaagttt gagcagctaa agcgcttaaa
    50881 ccagcggcct gggcaaaaaa atgaaaatgc tgatctttac tcagaataat agtaaataca
    50941 aaattaaaaa ccaaaacagc taaagataaa atcggcggta aaaaagcccg ccgcgccgag
    51001 ccgacgccat cgataccgaa atcaacatta taatgagaca cgaataaatc tgagccgacc
    51061 gcgataaata ggcgatacgc caaaaaccaa agaattattt gcaataccaa cccgatcaat
    51121 aaataccagc gattaggtcg ataatgccaa taaattttcc aactagcccc ccaataactt
    51181 ttaaaattat accagcgtcc gtataaagac ataaacaaaa aaataaagct aaataataaa
    51241 atatctttgc gcttattgta gcatattccc agctatttta aaaatcaagg agatgatgat
    51301 ataataaaaa tatgtctgaa aaaatagcca aaacaatctg ccgctacggc caggcaaacg
    51361 gtcattctca cttacgcctg gagaataaag aaggcaaact tctctgtttt tatggtcccg
    51421 agaccggagc ggaatatttg cgtcttgact gcagtgaagc cgcaagcatt gctgaaactt
    51481 tccgatattt agtcggcgcc gttgataatg agctctttgt cgacaaacgc tttaaaatcg
    51541 cggataaaga cggcgttatt agcggtcgcg ctactctgtt accggccagt aacggcgaaa
    51601 aactattaat caccctcaga aactccgagc cgcaagctag acgttggggc gctttagggc
    51661 taaatcgcga ccaacaaaag atactaaaat cggctttagc caaaaaaagc ggcgtgatta
    51721 tcatcgctag cgccgccgaa aacggcagca gcagcaccta ttattctctt cttcaagcgg
    51781 caaataataa taaaagcact tattcgctag aagcatatcc cggcaatagc ttaaataaca
    51841 ccaatatcat taaccccaaa aaatacggag gaatagccgc ctctttagat aaattaataa
    51901 gcctagacag cgagttaatt ggcgtcgacg ccgacctcaa tcaaaatgat atcaaagcaa
    51961 tttggcgagt cgcctatagc ggccgactaa ttattgctgt cttaccggca cttaacgccg
    52021 cccaggcctt gaaaacaatc aaacaatcag gcttaagtac cgccgctatt gcttcccagc
    52081 tatctttaat tcttgctcaa aaattatttc cgcgcccttg ttctaaatgc ttgcgtgtct
    52141 ttgaccccgg cataaatatt aaaaaaacaa ttatccagcg ctggccggta gccgctacgg
    52201 cttggcctaa aaaattatat cgcaatcatg gctgcttagc ctgccgtccg caagcgaacg
    52261 ccaccgcagt atttgaaata atgcgcttct tgccggatgg ccgcttggct gccggctatc
    52321 aaccccttat tatcgacgct ttgaccaaag ccggtctagg tctgattaat atcgaagaca
    52381 tcgccgcctg ggcggatacg aataaaaagt tatgaaaata atctgcgtta ttcccgccta
    52441 taacgaagac cagcacctag ccggcgtcat cgccaaagtc cggccactgg ttgacgctgt
    52501 cgttgtggtc gatgatggct ctcaaaaaaa gattacaggg ctggcagaac ttgccggcgt
    52561 ttttgtctta agacacccaa ttaaccgcgg ccaaggggcg gccctacaaa ctggctccga
    52621 ttacgcctta aaattaggcg ctgacattat tatccacttt gacggcgatg accaatttga
    52681 agccgcggaa attccggaag tgattgcgcc tttgttaagc caagaagccg atgtcgtctt
    52741 cggttcccgc ttcttggaaa aaaaatcaaa catccctaaa gttaaaaaat atatagtgat
    52801 gcctctgggt cgtttcctta accggctctg gggagtgcgt accagtgatc cgcaaagcgg
    52861 cttccgcgcc tatactagag aagtggccca aagcttccgt attgaaaatg accgcatggc
    52921 ccactgtagt gaaatcttaa ttaagctcag tcaggctaaa tggcgtataa aagaagtacc
    52981 cgtcaccgtt acctaccacg aatatggtca gaagctaagc ggcggtttcc gtatcatcaa
    53041 agatttgttc ctgcaaaaga ttagacaata aaaaagaggt gaaaaaaatc cacctcttct
    53101 tggtcaaccg aaaaggctac caaggaataa gcgttggcag caaataatca ccggcggcga
    53161 taatcgccgt gatgaaaatc agccaagccg gcaccagcca taatatatcc tgtctttttt
    53221 ttgtttctct gcaggcagat actaagtcag tcataaaaat aatggcagat aagataaata
    53281 tcaacaaaac ggttaatagt gcaatcttat cgtcatttaa atcttgaaac catctgaccg
    53341 caaagatacc agggtcaaac ttagtccagg taaacaacga gtacccagac atagacagaa
    53401 aagaaatact taggggcaag ttagcttcac ccataatttt ggggaaaatt acccacacaa
    53461 aagcgagcac cgccaaaaaa attgagaaca aatccttgcc ctgagtggcc atttcaggat
    53521 tagacagaaa aaccctgaca agggcgtaag cgagaacgaa ccaaatcagc cctttaatct
    53581 ggttctcaaa aaactttttc attttcttgg gatttattag ggaaacaatt ataatgaact
    53641 acaaattata tcatcataaa acaataatta atttttgtca atcatatgct ccaacaagct
    53701 attgctctcg cctttatcgc gattttcatc tggcgcctaa gccagcaaaa aaagaaaaag
    53761 caaatcggca ataatgagtt tattctctgg ctcgctgttt ggctaatagg cgctctggcc
    53821 atcattttta tccgctctct tgataaaatt ctggtaagcc tcggcttctc cggctccggt
    53881 atcaatttcc tgctctatct ggctgttatc agtctctttt atttagtctt caagcttcgc
    53941 ttaaaaatca gcaaactgga aaaagatcta accgacttag taagagagat tactctaaca
    54001 aaataaagga acttaactat gaaaatcgcc caaatcgtct gtgccttccc tccttatgcc
    54061 ggtggcattg gtcaaagcgc ctaccgtctt ggagaaattt taagccgcga acatcaagtt
    54121 tccactttta ccctggtggc ggccaaagaa aaaaatcctc aaaaaccaga agcccggcaa
    54181 aaaatcattt tcctgtatcc ccacctgcgc gccggccatg gcgcctttcc ccttttcctg
    54241 ccctttaggt tactctctta cgacttaatt tatcttcatt atcctttctt tggcgctgac
    54301 gctttaattt tactattaag ctgcctgcgc cggaaacaaa gattaattat tcattatcac
    54361 atggacacac cggctttacc cggatttaaa aaagtgctcg ccctcccttc acgtttaatt
    54421 ttgccgcggc taattaaaaa agcggaaaaa atcatagttt ccagtcaaga ttatgctgat
    54481 aacggtaatt tagccaaaat cgctaaactg tacccggaaa aaatcaaagc catacccttc
    54541 ggcgtagaaa cagacatctt ccggccaaaa ataaaaacgg ataatagcaa agtcgttaac
    54601 caagcagtcg ctctcgtcaa ttttgtcacc cgtcgtttca ttaaaagagg cggacacacg
    54661 cttttgttta tcggcggttt ggaccaagcg cattatttta aaggcttaag tttattatta
    54721 aaatcaatga aattaattac cagcaccgtt actctcaata ttatcggtac cggtgaatta
    54781 aagaatcaat acgaaaaaga agttaaatct ttaggattaa gcaatcgggt taatttttta
    54841 ggaaaagtca gtgaagatga gttaatccgc caatatcaaa atgccgacct ccttattctc
    54901 ccttccatta acgaacatga agctttcggc ttagtcttaa tagaagcgat ggcctgcggc
    54961 gtgcccgtta ttgccagtcg cctgccgggc gtacgcagcg tctttgaaga cggtaaacaa
    55021 ggctattttt gcgaaccggg caatgtccgc gatttagccg aaaaaataga ccagctatgc
    55081 cgggatgaaa ataaaagaca agcgatggga caggcagcta gaattctggc taaagacaag
    55141 tatgactggg aaaagatagc cgaccagata ttagacgaat tttcagacaa tagataatat
    55201 atgattatcg gcatggccaa caatcttttt ggaaaatatg gacgcggcag cggcgcggag
    55261 gtgattgttg aaaaaatggc cgctgattat cgccagcaag gtcatcaagt cttcattatc
    55321 accaccaagc ccaaaggagc ggctgacggt gaccgcgatg attgctatta tcttccgtca
    55381 aattatcacc gcttgaaaga ttggtcgact cctagaaaat tcctatggca tgccggccag
    55441 cttttattgc ctggaaattg ccgaaaaatt aaaaaaatac tgcaagcaag aaaaccggat
    55501 ttatttatta cccacaatct tctcggcctc ggcgccagtc ttccccgcct tttaaaaaaa
    55561 tacaacattc gacacgaaca cgtactccat gatattcaac tattacatcc gtcgggtttg
    55621 atgtattggg gacaagaaca tataatcagt agtccggccg ccaaaatcta ccaagcgctc
    55681 accaaacatt tactgaaaaa cgcgagtctt attatttcac cttcatactg gttgcttgcc
    55741 ctccatagtc aacacggatt ctttcttaat cagactaaaa aaataagacg aaattttaat
    55801 ttaaaaaaaa taactccatc cactttaaag aaaccgataa atttcgtctt caccggtcaa
    55861 atagaaaaac ataaagggat aaatatcttg ctctcggcat ggaaacaagc caatctggcg
    55921 ccagaaacag ccaaactgat tatcgccgga gacggctctt gggcggactg gttaaaaaaa
    55981 gaagtagccc taattaataa tattgagtat gtcggccgcc ttgaccgcgc tggcattact
    56041 gcgctattgg aaaaatctga tgtggtcgtt ttgccttctt tagtttatga aaattctccc
    56101 accagccttt gggaggcggc agcggccgga aaacgcgcaa tcgcttccga tatcggtggc
    56161 attccggaat tatcccccta tctcaacttg cttctaattc ctcccggcga cgtttatgcc
    56221 ttagcggaag cctttaaaac aattactcac taagataaaa aaacctccgc ttcagcagag
    56281 gttttaaatt attaaaacct ataaatttct aagcttgaaa cccgcgcaag cgcttctgcg
    56341 cctgaagagc aaagatcgtc ttattcaatc cgttttcaag agtaacaacc ggcatccagc
    56401 ctaaattttc ccgagccagc ctgatatcgg gtaaaaccag ttcgctcata aataagcgct
    56461 catccttata gactatacgg gaatcggaat tagtcgccct gataatcatc atcgccaaat
    56521 cagtaaaatt aacatcaaca tcagaaccaa tgtttattgg acccgcttct ttagaaccca
    56581 tcaatttaac cgcggcatca atagcatcat caacataaca gaaagaagaa gaaaatctgt
    56641 cgtcgccaaa aattactaaa tcttttccat ctaaagcgtt attgataaaa tcaggaatca
    56701 tttggtcgtc attaagttcc atgcgcggac cgtagacacg aaaaagccga ataatcttgg
    56761 tgtctaaccc aaatacttca tgataattaa ccaccaaagt ctcggcaaaa cgcttgccct
    56821 catcataaca actgcgtgac gaaagctggt caacaagccc gagttcattt tctgaaactt
    56881 tacgatcagc cgctcccact tgacgcggac catagacaac cgaagaagaa aactgcataa
    56941 atttagcttt agaagctaaa gctaaatcaa gcaaattaat tacgccgaga gaattagcag
    57001 ctagagtggc cacgcgattt tccaaaaaac gacgaggcga cattggacaa gctaaattat
    57061 atatctcctg caaaccctga aattccaatt taaatttctt taattccggc aaagcggacc
    57121 aattgagggg cttagaaata tcatgattaa tgaaaacaaa gtccggatta gaaagaaggt
    57181 ggtcaatatt gctctcactg ccactagaaa aattatcaac gcagatgact ttacaggttt
    57241 taaccagttc atcgcaaaga tgagagccga taaaaccggc gccgccagct acgagcacat
    57301 tctttttttc aaaaattact tgtttgggca tatgattata aaacggatat aaataaattt
    57361 aagcaataaa aacaaactaa ttaataaaat taaccacacc aacgttaacg ccaccgctaa
    57421 aactctcagg aaaagcatag aaagactcca acaaatcacc cttgccacta aatacacgaa
    57481 catgcggagt ggctccaggt ttggcgccaa ccacaatatc agcacggcca tcattatcaa
    57541 gatcgccggc ggcgacactg aagcctccct gccaattgcg cccgtaggcc ataaaagtct
    57601 tcttaagttc acctttattg gaaaaaatct ttaaccaagg ttctcggccg aaaccaggag
    57661 caataataat ctcatcctta taacgatcag agcgtccgtc aacattagcg accgccacct
    57721 gcagaccccc cttaaagttt ttttcaaaag caaaaaattg gccctgcaaa tgtcctcgtc
    57781 gatcgaaaat ttttacttcg ggagcaccgc cttgcccagg aacggcaata atttccgcag
    57841 ctccttgtcc atcgatattg ccagccgcca cattaacccc cccgcgaaaa ttcttagcaa
    57901 aagcaaaaaa ttgacctttt aataggccct tatggtcaaa aatacgcaca tgcggaccgc
    57961 cgccagcccc agcgccaacc actatttctg ccaaaccatc atcgttaaca tcggctacgg
    58021 ccaaactaaa accaccttta aaattgcgtt cgtagactaa aaatttacca cgtaaagtac
    58081 catcagggcc ataaatactc acataaggtt cttggccggc gggagcggcg ctaatgattt
    58141 catcctcacc gttaccatca acatcaccgg cggcaaaaat acctccagcc gcttcggata
    58201 gaggccaggc atccaaaata acaccgcttt tatcacgtat ttccaaactg ctgctagccg
    58261 ttgtcgtggc cggacttatc attaaacgtg ccagatgaga atacaggcct tcttgagcaa
    58321 cggtcaccgc tctttccaga ttaacgcgac ccgaaccgag ttgaccgaga taattgggat
    58381 taaggcgact aatgttatcc gttgtctgta aaagtatttt gacaatttcg gcaggtctta
    58441 attgaggatt agcttcggct attaaagcta aggagccgct aatcaccgcc gcggccattg
    58501 aagtgccgga ccaaaaacca tcataatatt tatcaggatc attaggattg ccaccatgcg
    58561 taatcgtgct atagaaactg acgccaggag cggtcaggtc aatgcatttg aagccgtagc
    58621 tggaaaaatc agttttctga tctagggcat cagtcgccgc tactcctatt accatatttt
    58681 ctccgttttt accatcataa caagccggat aaatcggtgt cttgtccgtg ttataagctt
    58741 cgccgtcttg ttgctcatta ccggcggccg cgacgacgat aacaccggcg tcataagcgc
    58801 gttgaatagc gtcttgtaaa gcctggctat aagtaaaacc gacaaaactt aaattaataa
    58861 tatcagcccc attatgaacg gcgtaatcaa ttgctctgac caccgcgcca atatggcctt
    58921 cacctttatc attaagggcg cgcaagggca ttatttttgc tttccaggta acgccgctaa
    58981 tgcctttata attattaccg accgccgcca ttattcccgc caccatcgtc ccgtgagaaa
    59041 caccggactc cgtccagcct tcatcgaatt taggcgaagg atcaggcaaa ttctgaacaa
    59101 aatcccatcc tgaaacatcg tcaataaaac cgttgcggtc gtcatcaatc tgattattag
    59161 gaatctcctg acggttaatc caaatattat cgcgcaaatc gggatggtca atctctacac
    59221 cggcgtcaat cactgccacc gttatattcg gactgctgtt aattttttcc caagcggccg
    59281 gagctttaat gcgagtaaga taccattggt tgccataata agaatcattc ggcaaaaaac
    59341 tggcgctaac ggccaacggc gctaataata aaaagacaaa aaaagaggct aaaaatggtt
    59401 tcatagcatc tttatcataa catatttatc agacaattca aagataaaaa aatgggctca
    59461 gcccattttt atctttcagt taatatcttt aaagtttctc tggctgattg ttcccaacta
    59521 aattgatttg cccgggctaa tcccctagaa attaaatctc ggcgcaaatt ttcattaccg
    59581 gcgacagtgg ctaaagaggc ggctagttca ttcttgtctc gaggattaaa ataaatgggt
    59641 gcctccccta atatttctct caaaaccggg atatcggaaa ctaaggtcgg cacgccgcaa
    59701 gccatcgcct gtaagacggg gataccgaag ccctcgtaca gagtcgggaa aacaaaagcc
    59761 aaagcggcgc taaagataat tggtaaatcc tcttccgcta cccagccagg cattaaaacc
    59821 tgacgactaa gattatattc ttcaataata tatttcactt cgtcgtaacc aaaaccggca
    59881 ttgcctatta aaactaaact gatatcactg tgaggatttg tttctttaaa taaagcaaaa
    59941 gcctccacca gggcttgggt gtttttcttc ttttccaagc ggccaacgta caagaaaaaa
    60001 ggctcctcaa gtccatattt ttctaaaata agacgacgct cctctttatc ggtacgaaca
    60061 aaataaagtt cgcgggcata accgttatga accaccttaa tcttttctgc atatttctga
    60121 gaataaacat caagaatttc ttgacgagta aattcggaaa cagcaataat tgtgcgggca
    60181 tgttttaaag cataggctgt agaccaaacc aaataatcga aaggacttga ctcatagcga
    60241 ccgcgggtaa acaaacgcgt caacaaattt aaaaaacgtt tgcccgtttt tgataccaag
    60301 cgtaatcccc gacgttgata taagccccct tcacgcagaa aggcaatatc gtgaatggta
    60361 ttaaccgtat ggcgcggtcg gaataaaggt aaagaatgag cgggcacaaa aagcacgtca
    60421 gggcgcctca caatcatttc ccaagacaaa cggccaagag tccaaaaata tcgccaaggc
    60481 caatggagcc ggcgtaattg aaaattagga taaggcgcga tgactgcctg taaatcagga
    60541 gaaacttcct tgtctaaata aagaatatat ttgtttttac gatcaagccg ggctaaatgc
    60601 ttaatgatat aaaaagaata ccactctgtt cccgtgcgca aagatcgatt ggcgcgagaa
    60661 gcgtcaatgc cgataatcat ataggtaaaa ctatttggtg gccaaaatac tacgcttata
    60721 agaatcgaga ttgtccaaag caattcctgt tcctttggct acgcataata aagcgtcatc
    60781 agcaatatag gcagggacac cggtagttcg agccactaac tgatcaatgt tgcgtaaaag
    60841 agcgctgccg ccggacaaaa taatgccttt atccataata tccgccgcta attccggcgg
    60901 ggtcttttgc aacactcttt tcgcggcggc aataatcgct tccaattcat tctggatagc
    60961 ttcggtgaca tcgtcgctag agacggtaat gcttctgggc aaaccggtaa cgatatccct
    61021 gccccttatt tccatataca gcttatcttc tatgtataaa gcggaaccaa tatttatctt
    61081 aatctcttcc gccgtccggt cgccgattgc gagattatat tttttgcgga tatgctccaa
    61141 aatagcggca tcaaatttat taccgccgac gcgcactgaa gtcgaggcta caattcctcc
    61201 cagagaaata acagccattt ccgccgtgcc gccgccgata tcaataatca tgtgtccagt
    61261 agccgatccg atgggaatat cggcaccaat agcggccgcc acgggttctt tgataatata
    61321 tgcagcttta gctccggcgg cgatagtcgc ctcaattact gcccgccttt cggttgaaga
    61381 aattcccgcc gggacagcca ccattacctc tggacgaaaa agacggacac cgcctaaagt
    61441 tttattaata aaataacgaa tcatcgcctc ggttgtccga taatcggcaa tcacaccatc
    61501 ttttaaagga cgggcggcca caattgtgtc tggcgtccgc cccagcatat ccttagcctc
    61561 attaccgact gccaaaatct tattgtcgtc aagagaaata gccaccaccg aaggttcgtt
    61621 aataacgatg ctccgacgtg gtaaatatac taaggtataa gttgttccca aatcaatacc
    61681 gatgcgtttt ataaacataa attaggctaa aataaaagaa aaaatttcag aaactgagat
    61741 tcctcctata aaactatatg ttaccccaaa ttccctggtc ggtcaaatgg agaatattat
    61801 tcttcgtaag tatccgcaac ttcgctattt tcattctcag tctggttggc gatgattttt
    61861 tttacttttt ccgcaaattc ataggtgtca aaatcatctt taataaaata ataatccgct
    61921 cccaactcta aactgcggtt tttactgtcc tcatcgctta aatcggtaaa aataaaaatg
    61981 gataaattag ataattcttc atcttcttta attctcttta taatttcaaa gccgtcaagt
    62041 ttaggtaaaa ttaaatctaa aatcatataa tcaggagaaa aagaccggat attgtccaat
    62101 aattcttcca gttcctcttc cccttcgctc gactcgacga caaattcatc ggcgctgaac
    62161 tgagcttcca gcccataaag aaggttgaca tcatcctcga gaataaaaat ttttttatta
    62221 gccatatgat ttatcagatt caaagattaa tcgtcattga atcttattta attttttgtt
    62281 ttagcattaa taatgggtcg ccaccaatcc tcatgctggc gataccaatc aacggtttcg
    62341 gccaaaccac gttcaaaaac gattaatggt tcccaaccta aatctctctt ggctttacta
    62401 aaatcaacgg catagcgtaa atcatgcccg ggacgatcgg ccacatagtt aatcatttcc
    62461 tctccctgac caaaaataga tagcaacttc ttggtaattt ccagattgct gcgctcgctg
    62521 ttgcccccca agcaataggt ttcgccaatt tttcctttct gtaaaatcaa atcaatgccc
    62581 cggttatgat catctacatg aatccaatca cggacattgc ttccccgacc ataaacaggc
    62641 aacttttttc cgtcaattaa attagtgata aagagaggaa tcagcttttc tggatattga
    62701 taaggaccat aattattgga gcaattggaa atagtaatcg gcaaggcatg ggtatagaaa
    62761 taagctctta ctaaatgatc ggaagcggcc ttagaagcgc tataaggaga acgcgggtta
    62821 tagggggtat tttcattaaa acggccacca tcaagaggca gggtgccaaa gacttcatca
    62881 gtggaaatgt ggtgaaatct aacaccgccg tgtttttggg ccgcgtccaa aagcacacgc
    62941 gtcccctcta cattagtacg aacaaaatcg gcgctattta aaatagaacg gtcaacatga
    63001 ctttcggcgg caaagtgaac aatcgcgtca acttccgcta ctaaactgtc aaccaaatcg
    63061 atatcggtaa tatcaccgcg cacaaaacga taagcataat tattatctaa atcgcgcaaa
    63121 ttttccagat tgccggcata gctcaaaaca tccaagttga taatcctgtc gtcaggatga
    63181 ttcttcaacc aataatgaat aaagttggag ccgataaaac cggcgccacc ggtcactaat
    63241 aatttcataa aaccgctttt aatctagagc aaattgcttc cgcctctccg ggagcataag
    63301 cttctttccg ccataaaggc aaattgtcgc cggcaattcg gggaatgaca tggaaatgga
    63361 gatggggaat ttcttgacca gcgaccggat cattattttc aaatacgtta tagccggcat
    63421 aggccagttt cgtctttaac attaaaccgc atttttttac caccgtaatc aaagactgta
    63481 attcttcatc cgggatctct tccaaattag catagtgcct cttaggaact actaagaggt
    63541 gacctttatg aacgggcgct atatccaaaa aagctaaaac ctgaccgtct tcgtaaacct
    63601 taaaagaagg aatttcgccg gctattattt tacaaaaaag acacataatt ttaatttatt
    63661 ttataaggac tgtccggatt ctgttcccaa cgaatttcat cgatttcgtc ctgttttaaa
    63721 acacctttat agagtttgtc cggcaaattt atcgaaagag ccggcactcc agaaagacac
    63781 tgatatccat ggacaacgcc gggcggaacc aggacggagc aaggattatc ttcgccaact
    63841 tctatttcca ttttttcgcc tttagtcgaa gaatcagcgc gacgatccca gagatgcaaa
    63901 cgaaaacggc ccgggccgat aaagataaaa aaatcacttt gataaacgtg ctcatgcggg
    63961 ccgcgaatta ctccaggtaa agtcaagctg acataagaca tcgccggagc caaatcactc
    64021 tcatcgcgac gatatatttc catcagccaa ccgcggtcat cgctaaattt ttttattttc
    64081 ttaataatca cccctttaat catatgattc aaaaaaataa taaattaggc tattttattt
    64141 ggcgcaaatc caataatccg ttctgggctc cgaaataagc gattttagaa gcggtattct
    64201 tggcccccaa atacaaagcc cgcttaatat cgccgccact aagttcccaa ccggcgacaa
    64261 aagaagaatt aaagacatcg ccgacaccgg tgatatcaac ccttttcttc tccggaaaaa
    64321 ccgcctgatg atagaaatta tgaccatcat aaacatcggc tccatcctgc ccactggtaa
    64381 ttaaaacgat ttccggacca gccgccttta aagcgactaa aagattcttc acattattta
    64441 gaaaggccgg actctggccg cgataacggg catcacttaa caccaattca atcgcctcat
    64501 ctttattaac acaaaaaacc gaagttcttt ttaagaaaga agctattttt ttcaaaccag
    64561 cctgatactg agacgccccc ggattccaag cgattttcgg gccagaaaga gaaaaaattt
    64621 tcctggctgc cggcaaccaa tcgcctggca aagaagccaa atacaaccaa tcggcttgct
    64681 ttaaaattct ttcttcttgc cggcttattc gtaaatcatt atgagcggcg cgactggtaa
    64741 aaattatccg ttcgccgcca ggggtgacaa gaataaaaga gatcatagtc tccctgttct
    64801 tttctgacga caccaaagaa gtgatgacgc catgctggcg cagattagca ataatggctc
    64861 ggccgctttc atcttgaccg actctagcga gacaagcggt tttaaatcca gacacggcaa
    64921 aatttacagc cgcattggcg gcgccgccgc cgaataaaaa ataaaagcga tcaattttaa
    64981 ttttcgctcc gtactcaaaa gctaaaagct tctggcgcaa aacatcgcgc tgattatcta
    65041 tgagaacccc ctcatcggta aaaaaagtgg tgtcgcgagt cgccccgccg accgtaatga
    65101 tatgattttt cataaaataa agctttattc tatcataaca cgaattaagg ccttttcaaa
    65161 aaaaatggct taagccgccg accggccagg gcaagtaaat taaaaaacag gccaaccaaa
    65221 aaaagactga ttacggcggt ggcggctaaa tctttccaaa aataatctga aaataaaaat
    65281 aacttttcgg cagaacctga ccgggcaaaa ataagtaaca gtaaattata actcaacgcc
    65341 tcgactaaaa cggcgatgac agtaagtaat aaaaaagcat ataatgaatg attggtcaac
    65401 cagttttcta aaactagata aaccacggcc gtactaaaca agagggataa taaagcgact
    65461 ccgaaagggt gaaaacccaa aacatccaga aaccaaccag cggccaacgc caaaaaccaa
    65521 acctgatcct tgttaaaaat cagaaaagcg gaaataatgg ccacaattat caaattcaca
    65581 ctattccaag gaaacgacca agcgctcact aacgaaaact gaataatggc caagaaaccg
    65641 gccaagatca atagggcaaa atatgtgaaa atctggcgcg tcataatcag ggtaaaataa
    65701 ctgaaactat tgtcaaacta tgccaagaag aaagaggctc aatagtaaca tcttgccaaa
    65761 tctcattact ttgcttattg acttgggcta ctcgaccgat aactaaacca cggggaatat
    65821 cgccaccaag gccagaggtg ataataatat cgccttggtt tatctcctca agctggggaa
    65881 tataattcat tttaatggtc aaacccagtt ccccttcggc taaaccgatg gttttagtgt
    65941 tattaataat agccgccggc aactgacaat tagtgctggt agttaaacaa gcacgagcgc
    66001 tgtggtcctt cacctcgact attttcccta taatcactcc actctcatca atcacgccca
    66061 aaccgggacg caagccttca gccgcgcctt tgtccaaaac aatatcctga gcgtcttcgg
    66121 cggattcaaa caaattttga cgagaaacaa tattggcgct taacacctga tagttattag
    66181 cgttaacgaa atctaattgc tttctcagtt tattgttttc ctcctctaac agctgcaatt
    66241 tggcttggtc aacgatagcg gccgctaaat ctgctttagc tttatccaat tgctctaata
    66301 atgtggaaac gtccgtggcg gactcaggcc gtccgcgcga ccgttcccct aatctttgca
    66361 gagcggcggc gaaaggctta atcgccaaaa ataaataatt ctccaaaggg cgagttaaac
    66421 cggaaaaatg taaaataaaa atcagggcta aagaagctag ccaataccag atttttcttg
    66481 atttcccaaa tgagcgcata actcctctat ttatccaaag aaacattacc caaagtcact
    66541 ttagctaaca attcggaatc actaatgaga atcccggcgc cgcgaacaac acaagtaaga
    66601 gggtcatcga ctattttcac cggaatcttg gtggctagag aaatagcctg atttaaaccg
    66661 cgcaacaaag cgccgccgcc agacaaatag actccgcgct cataaatatc ggacacgagt
    66721 tcgggcggag ttgtctcgag aatagtttta atattttcga taatcttgct gacagtccgg
    66781 cctatcgcct ctctaatctg cttgtcattg acaataatgg ctttaggcaa gccgttaatc
    66841 agatcccttc cccgcacttc catttcctgt gattcttcgc cagcgatagc ggagccaatc
    66901 ttaattttaa tttcttcagc caattgttcg ccgattaaaa tattaaattc ttctcgaata
    66961 tactcaataa tattgttatc aaattcgttg ccagccaagc gtagagactt ccaggtgaca
    67021 accccgccca aagaaataac agcaatttcc gtactgccgc cgccgatatc gactaccatc
    67081 gtggccgtag cttctgtcac cggcaaatga gcgccaacgg ctgaggccat ggcttcttcc
    67141 actaaataaa ctttacgcgc tcccgcggat ttaactgcat cttcaactgc ctttttttct
    67201 acctcggtca aatctaaagg aatgccgata actacccggg ggcgagggaa aagagcaaaa
    67261 tgttcacgat ggactttatt gataaaatat ttgagcattt tttccgtcac ttcgaaatca
    67321 gaaattaccc cgtcaactaa aggtttgacc gcttcaatat gagccggagt ctttcccacc
    67381 atttttttag ccgcctcacc gacttctaag atttcgttag tacgcttatt aatagcgacc
    67441 accgagggtt cattgataat caagccttta tcactcataa ccaccaaggt attcttggtt
    67501 cccaaatcaa taccaatgtc atggctaaat tttcccacta atttattgaa cataagcagt
    67561 aatatggtta aatttaatta tttctgtttc ttgagacacg cgtctcttta tttccactcc
    67621 gttctgaatc aacgtttttt gactaataac aagacgatag ggacaaccaa ttaaatcagc
    67681 ctcagcaaat ttctcacccg ccgaaacatc gcgatcatca taaagaacct caattccgtc
    67741 ttggcctaat ttttgatata ttttttctgc ttcttcgtta gcttttagag acaaaagatg
    67801 aacgcgaaaa ggggcgatac tctgcggcca aattataccc cgctcatcat ggcaaacttc
    67861 aaccactgtg cccataatac ggcttaaacc aagaccataa caacccataa caacgggctg
    67921 tttaacacca ttcttatcgc tgtaggataa agcaaaggca tctgagtatc tagtgcctaa
    67981 tttaaagata ttgccgactt caaccgcttt ctctttccgc caatctttat cgccacattg
    68041 agggcaaact gattgttcgg caatgatttc ctggttaaca gccaaatgac acttatcgca
    68101 gatataaatt aaatcttcac ccgctgccgt taaggtttgg aattcgtgtg aatatttaga
    68161 aaaagagccg ccagcagcaa aagtcaaata agtattatca ccaagaccaa cgcgattaaa
    68221 aatacgaacg taagcttgta taacctgctc ataatatcga tccaaatcaa cttgatcggc
    68281 atgaaaagaa tataaatctt tcatagtaaa ttcacggcca cgcagaagac cggccttagc
    68341 tcgcttctcg ttgcgaaatt ttgtctgtat ttgaaaaaca gcaaaaggca attcccgata
    68401 agaattaata aacttgccag ccaaaggagt aaccacttct tcatgggtcg gattaagagc
    68461 atattccttc ttatcagaag cagcaatttt aaagagaata tctaaagact cccaacggcc
    68521 ggttttctgc caattttctt tcggtgccaa agtgggcaga ttaatttctt gaccgccaat
    68581 agactgcatt tcttctctaa taatttgtct aatcttttcc attactcgcc agcctaatgg
    68641 caagaaggaa taaactccag ccgccacctt gtcaataaaa ccggcgcgca gcaaaacctg
    68701 cgcattatag cttgtttcgt ctttaggtaa ttccttaaga gtatgggtaa atagttgaga
    68761 ttgtttcata taaaaattac tgattttttc ggcttaactt tttcaaggcc gctttcactc
    68821 tttcgccgct atcttttaaa tcaacgtcaa gctcacccaa agcgttcctg gcgtcagaca
    68881 aagaatagcc gagcgccagc aaagcatcaa gttcatcatt aaaaccggag gaaaaggcgc
    68941 ttcccaaact agcgccatca atacttttga caatcttatt ttttaattcc aaaaccactc
    69001 tttccgccgt ctttttacca ataccggcga ctttggtcaa cagggcggca tcgccgcgaa
    69061 taaccgcttc cctgatatcg ccgatagaag cgacggagag aactcccaac gccgatttgg
    69121 ggccgacgcc ggaaacagaa agcaaaagct gaaataattc cagttcggct aaatttctaa
    69181 aaccatataa atcagaagca tctgcgcgca cttggtggga aagatagaat tcctgctctt
    69241 ctcccacttt tagcgacgac cagaaagctt cacccacaaa aatctgataa ccgacattat
    69301 ttgtttctaa tattagataa ttattacttt tagccaaaat ccggccacga agataagcaa
    69361 acatatgctt ttagtataat atatccgaag atttaattaa atcttgacag aaataaacga
    69421 taatgattat tgcttgctaa aagctccgcc ctcaaggcgg cacttatgtt tgcaaacgtt
    69481 tcccgcgaat aaggccaaat aggtttttaa tctttaaatt aaacctccct taatttggca
    69541 atccgctcgg ctactggcgg atgagtcatc catattttag caaaccaaga aacttgattt
    69601 tttttctcct ggcctttaaa aggagaaaca atatacatat gggcggtagc gcgggaagcg
    69661 actttcaggg gttcgcggtc ggcgctgatt ttttccaaag ctcgcgccaa gccttccggg
    69721 tagcgggtta aaagagcggc gtcggcgtcg gcgacaaatt cacgctttct ggatacgacc
    69781 agctgtagca gttgggcaaa aataggagcg aggatcgaaa aaacaagtgc caaaatcagc
    69841 ataatcactc ccagctgatt attgttattg ttatcattat gacggcggcc gccaaaaaat
    69901 gaccaacggc ggaaccaatc ggccaataaa actacgatac cgactaaaac tgtggccata
    69961 gtggagataa ggatgtcgcg gttgccgata tgggataatt catgcgcaat aacgccttcc
    70021 aattccgctt tctcaagctt gcctaaaata ccgctggtaa cggcaatcgc tgcgtgctga
    70081 gggtcgcgac cggtagcaaa agcattggga gcgctgtctt taattatata aatcctaggc
    70141 acgggcaaac cggcagttat acaaagattc tccaccaaac gataaagctc gcggccatta
    70201 ttcttgttta cctcttttgc tcccgacatg gacaaaacaa tcttatcact ataccaataa
    70261 ctaaaaaaac tcattaaaat gctaaaaata accgccgccg gtaaaattat gggagcgtcc
    70321 atggcttgag aaaaaatata tccgattaag ataacaaaga tgaaaaaccc ggtcatcaag
    70381 aaccaggttt ttctttggtt gacagcgctg taattataca gattggacat aaataataat
    70441 agttatttat tctcattatt catcttctgg gcaaagcctt caaaggcttt caatacttca
    70501 agcggcgtca taaagataac agtattcgac tgatcagagg ataaatcatt aatagactgc
    70561 aaggtgcgga gatgaagagc ccccggaact tcagataaag tgcgggcggc ctgggcaatg
    70621 ttggccgaag cggctaattc accctcagaa ttaattataa ccgctctctt ttcacgctcc
    70681 gcttccgcct gcttggcgat tgtcctctcc attgattccg gtaaagaaac gtcttttagt
    70741 tcgacgttgt taactttaat cccccattca tcggtttcct tatcgacaat ctccttaatc
    70801 cgatcggcaa tttcgtcgcg cttgcttaaa agctcatcca aagtaacaga gccaacaata
    70861 ttacgcatcg tggtctgagc atattgggaa atagcgtatt taaaattttc cacctcaata
    70921 atcgctttct cggccgccgt taccttataa taaataacgg cattaacggt aaccggcaca
    70981 ttgtcgcgag taatcgcttt ctggtcggga acgtccaccg ccttgacgcg aatatcaact
    71041 ttatgaaaag tttgaaagac cggaatcaat aagcgccatc cgggctgcat gacgtaagaa
    71101 tatcgcccca tggtaaagac gacaccgcgc tcatactggt taacctgttt taaaaaggcc
    71161 aacaagagaa cgccgataaa aataataaga taaatccaca tatagttata aatctaaact
    71221 tagcgtcaac ccgcttttgc tcagaataaa ataaagacct agaattttac cgctaccgct
    71281 tctttctccg cctcgaccgc ttcaaagaaa ttataagcct tgaatcctaa ctgtttggcc
    71341 aagatattgg tcgggaaaac ttgcaatttt gtattgaaat cacgaacatt ggcattataa
    71401 aagcgacggg aagccataat tttatcctca gtatcagtca aatcgcgctg aagttccaaa
    71461 aagttttggt tagctttcag gtcaggataa ctctccgcca aagcgaagat ggattttaaa
    71521 gttgaagata aagcattttc cgcttcaagt accgccttag ggtcacgact atcctgggct
    71581 tgcacggctg tgttcctggc tttaataaca ttatccaaag tgctttgttc atgagtggca
    71641 tagcctttga ccgtttccac caagttaaaa atgaggtcat gacggcgttt cagctgcacg
    71701 tcgacatcgc tccaagcttc gtccacccga ttctttgaac gaattaagcc gttgtacatc
    71761 gctattaata ccaaagcgag taaaacgata atgcctaaaa tgatccaagt agtcataaaa
    71821 gataggaccg gaaaaggatt ctccggcaaa ttaataaatt agcctaataa ttatagtata
    71881 atttttgatt tttgtctaaa aaaccgaatt aaagcttaac tatctgcaaa gacaaatcta
    71941 tttgttccaa tttaagaact ttaacagcca aaggattatc ttctcgaccc tgtctaatca
    72001 catcggctaa agtcgctttt tttaattcct cgctccaacc ggccagccaa gaaaaagact
    72061 cttcactgct ctcaattaaa atttctatcc tatcggccac ggttagtccc gcttctttgc
    72121 gcaaggaatt aatggttctg atgatttctc ttttgttgcc ctccctctgt aattccagag
    72181 taataaccgt atcaagttta acctcaagac tgccaccgcc cccttgccaa ctcacactgc
    72241 gcacattaag ctcttccgac aacaaaacaa aatattgttc agctaatttc ccgccgccgg
    72301 taaattccgc cgaatttaac atttgcctta ttttaatacc ggccgcatca cgcgctgaca
    72361 aagccaactc caccccttta cgggccaatt gcatatttgc caagacggct tcttcctctt
    72421 cggtcaattg gccgagattg ggccaagact gcaagtgtac cgattcatcg cgcttttcca
    72481 acttatatcc ggatacttcc tgccataaac gttcagcaat aaaaggcata aagggagcca
    72541 taatcttagc cagcgtcaat aaaacaaaac gtgtcatcgc taaaaccgcc tgcttgtcgc
    72601 tttcaacttc actcttaaaa cgttcgcggc tacggcgcaa ataccaggtg gaaagttcgt
    72661 cgataaattc cgtaatcgga cgcatcgctt taggcagatt ataggcctcc atcgccgccg
    72721 ttacttcaat atttaaaact ttcaactttg ccaaaatcca agcatcaaga atattctccg
    72781 ccgttacttc ccgaccatca tattcgccgg cataaatttc atagaagcga tagacattcc
    72841 acagcgtcat tacgttcttg cgcaatgact cttgcactcc gctttcggta aaattgaggt
    72901 tctccgcctg catgaccggc gaagacaaaa gataggcacg caaagcatcg gcgccgtatt
    72961 tttccataac cagggacggg tcgggataat tctttaattt tttggacatc tttttgccgt
    73021 cttcggctaa aacgataccg ttaacaataa catgcttaaa agcctcagtc tctttaatac
    73081 cgccggcaat aacatgcaaa taatagaacc aggctctggt ctgatcagcg ccttcggcaa
    73141 taaattgagc gggaaaattc ttctctaatt tttctttatt ctcaaaagga taatgtaatt
    73201 gcgcgtaggg catggagcca gactcgaacc agcaatcaat cacatcggta atccgtcgca
    73261 ttggcgctcc gcattccgga caagcgaaaa ccaaaggatc aactttatct ttatgaatat
    73321 catcgatatg cttaccggtt ctctcttcta attccgctac cgaagaaaca acgtggcgat
    73381 ggccggccgg gcattcccaa atcggaatgg cactggccca aaaacgctga cgagaaatcg
    73441 accaatcacg cgctccttcc agccaattgc cgaaacgccc gttcttgata taatcaggtg
    73501 accactttat ttctttggct gtttcccgta atttatcctt tatttttgtt acccccacga
    73561 accaagaaga agcggcatag ttaatcaagg gggtatcaca acgccaacag tgaggatagc
    73621 tgtgttcgta tttttcttta ctaaaaagaa gattatgtgc ggccaaatac ttgataattt
    73681 ccacatccgt cgccagatga tcttctcgtg gtttcacatg aagaccggga aaatcagcaa
    73741 cttccttctt aaacattccg tccaagccga catgctgaat gaaaggcaag gattcctgct
    73801 tgcctaaatt catatcatct tcaccgaaag cgggagcgat atgaacgacg ccagtgccgt
    73861 cctccgtagt cacgaaatcg gcggcatata ttttccagcc attatctcga ttctccagat
    73921 tcttatcctc agagtaataa ttaaagaggg gtttataggc ttggccgatt aaatccgagc
    73981 ctttgcaagc atcaagaact tcataatttt tatttttaaa tacttcttcc aagcgtccct
    74041 tggctaaaat ataattagct ccatcaaatt tcacttttac atactcaatc tccttcccta
    74101 ccgccaaagc aacattacct atcaaggtcc aaggagtggt ggtccaagca agtacgaaag
    74161 taccgggttc atcgactaat tcaaacttgg cgatagccga taaatcctta atatctttat
    74221 atccttctgt tacttccgac tgagataagg tcgtttcaca gcgcggacag atgtacatgg
    74281 agcgataatc tttataaatc aatcctttgt cccataactg cttaaaggtc caccagacgc
    74341 tttccatata attcaaatcc atggtcttat aagcattgtc aaagtctacc cagcggccga
    74401 gacggctaat cactctctcc cattctttgg catatgtcat caccttagcg cgacacatat
    74461 cattgaactt gccgactccc aattcttcaa tatctttctt attctttgtg cccaattctt
    74521 tctcaacaat attttcaatc ggcaaaccgt ggcagtccca gccccacttg cgctcaacgt
    74581 gatagccacg catagtctga tagcgaggaa caacgtcttt catcacactg gccacaatat
    74641 gtccataatg cggcgtaccc gtagcgaaag gcggtccgtc ataaaagaca taatcacctt
    74701 tcggcgaatc tttaactagg gatttatcaa aaatgccgcc atcttgccag aaggacaaaa
    74761 ttgaccgctc tgcttccgaa aaattggggg taactgtttt attttgattt tgctcattag
    74821 acataaactt gcttaaaaat aagttaatat atttattgat tatttttttc ttctccggaa
    74881 atttctagct aaaagatatg ggtctccatg ctgatggctt tagtgattcg gttttttgta
    74941 tctcgtgctg cgtgcttgtc caatcctttc gagtaatttc caagtcacca gccgcgataa
    75001 ggcctgtttg attgtggcct gaggtatttt accttttagg agtttgtcaa tttcggacac
    75061 gctaagttcg tcgttctttt cgaaaagttg ataaatttga gtttgctttt ccgagagaag
    75121 tttttctggc tgctcggatt ccatcagttt acgagctttc tcggattgct ccagtagaga
    75181 actaagtagg tattccaccc acggcgtaat atcctcgtgt ttggttttat gattcttttg
    75241 agtagctctc agggcaaggt agtattcggc tttcgtttgt tcaatgatct cgtcaaggga
    75301 aacgtaagga gtgtaactgt agcctgcctt aagaagcagg agatttgtga gtgctcgact
    75361 gattcttcca ttgccgtctt tgaaggggtg tatggccaga aattcaaaga taaagttggc
    75421 gatgatcaga agcggatgaa tttctttctt ctccaattgt tcattcgtcc aggcgatcgc
    75481 ttcctgcatt tccgctttaa cgagataagg aggagtcggg tcaaaaagca cgaccggttc
    75541 tccttgtcta tttgtcatca ctacagtatt gggggagtcc ttatattttc ctttatgcgt
    75601 ttggtctttt tctgaaaact gaaggaggat gccgtgaagt tgaagtatcc aaccctctgt
    75661 gagttttatt gttttccagt tatcaaagat acgtccgatt aaatcggcat agccagcgac
    75721 ttcctgttca tccctgctct taggtcgttt ccctttccat ccacgcagaa agcgggcgat
    75781 ctcctcgtcc gtcattttta ccccctcgat acgagtgctt gcacccgttg aagtgataat
    75841 gacccatgtt ttaagacgtc cgagtatttg tgggcttagt ctcagacttc cctgccaaag
    75901 tccttgaaat tgatcgatct tggcaatctt ggcgaggata ttttgggtca atttaatcct
    75961 cttgcttaat ctgttattgg tcataaattg gatataggtt aactatatcc aattatatac
    76021 gataataaat gattaatcaa gcgttgcttt ctgtcgctct ttttttctct ttgtttttta
    76081 gcatacaaaa acccaatctt ctcgactggg tttgctccta ttttctgttc tataattgga
    76141 cggaaacggc ttttttgtca gttcctacct tccatgacac ttcttatact tcttaccgct
    76201 accacaagga caggggtcat tgcgaccaac agtcttccca aattcgtctt ttaccttatc
    76261 tcttaccaaa tcaaaattat cacgtttggc gctcaatttt tccatttctt tagccgctcc
    76321 ttccaatttc aaattcttcg gttgctcact gccgcttaac cgtattaaag ccgaaggcgc
    76381 catcttcaga atatcgccgg tacgataaat ggaataaact aattgctgac ggataaggtc
    76441 tagcaattca tgatagaggc gataggcttc tttcttgtat tccaccaggg gatcacgctg
    76501 gccgtagccg cgcaaaccga tgccccggcg catataatcc attgtttcta aatgatcaat
    76561 ccacatctga tcaattgagc gcaaaagcag gcctttagcc acttccacaa aatcaattcc
    76621 cgcctgggta aaatcggata ctaaacgctc ataggtagcg gccgccaatt cctggacata
    76681 atccaagacc ttttggcgcc cagcctcgcc gccgtcgccg gctaattctt taagcttatc
    76741 ttcttgctca gaaacattaa gcatcgtccg taaagtctcc actatttctt taaaattcca
    76801 atcgccactc ttatcgccgg acgtatgaaa aacaataact ttagtgattt cctccttaac
    76861 ttgttcgttt ataagcgcgt ttaatttgat taaaggggta ttctcgcctt ctttcacttc
    76921 catttctgaa gcctccaaga tttcccggcg gcgacggtaa atcgcctcgc ggtgcttatt
    76981 gatgacatcg tcatattcaa ccaagtgttt gcgcaaatca aaattattac cctcaacctt
    77041 cttctgagcc gattccaatg atttggaaat catcctgttt tcaatcggca catccggcgg
    77101 taattttaaa gttttcatca tcgccttcat ccggtcgccg ccaaaaatac gcatcaaatc
    77161 atcttccgta gaaacataga actgggaaga accgggatcg ccctgacggc cagcccgacc
    77221 gcgcaactga ttatcaatac ggcgagcttc atgccgctcc gtgccaataa cgtgcaaacc
    77281 gccgagcgcc ttcacttttt cctgttctgc ttgcgcttca gcaagggccg cttcgtcatt
    77341 ggtatcgggg ttgcctccca agataatatc aacaccgcga ccggccatat ttgtagccag
    77401 ggtgatagcg ccgacccgac cggcctgagc gattatttcc gcttctttct gatgattctt
    77461 ggcgtttaac atctgcggat gcaagccctc gcgcgccatc atgttggcta aaagctcatt
    77521 cttttcaata gagatggtac cgattaacac cggctgcccc ttttcatgac gcgccttaat
    77581 ttcacggata acggcattaa acttattttc ttctgtccga tagattaaat cattcaagtc
    77641 cttgcgaata ttgggcttat tggtgggaat taccacggtt tccagtttat atatcttata
    77701 gaactcttcc gcctcggtta aagccgtgcc cgtcatacca gacagcttgt tatacatgcg
    77761 aaatagattt tgaaaagtaa tggtagccat cgtctgcgat tctttctgga ctttaactcc
    77821 ctctttcgct tcaatcgcct ggtgcagacc ttcgctataa cgacgaccat acatcaaacg
    77881 gccggtaaat tcatcgacaa tgataatctc gccatcctta acgacataat cacgatcacg
    77941 tttaaataaa accatcgcct ttaaagcctg ctccagatga tggacatcct tgacgccgcc
    78001 agacacatag atattaccga cacccagcca ttcttcaatc ttattaatac cggcctcggt
    78061 caaagtcgcc gccttcattt tttcatcaat attataatcc tcattctcag tcagaccttg
    78121 gactaattgc gcaaatttca catatttgtc agtggactcc tccgctgggg cgctgataat
    78181 aagcggggtg cgcgcctcat caatcaaaat cgaatcaatt tcatcaataa tggcataatg
    78241 taaagagcgc tggacagttt ggctaagagt ataggccata ttatcacgca gataatcaaa
    78301 accaaattca ttattggtgc cataagtaat gtcgcaataa taggcttcgc gacgagaaat
    78361 cggacggaaa tggcgcaaac gctcgtcata ctggctctcg tcgttatatt ccggatcata
    78421 tttgaaagcg gcctcatgaa caataacgct aacactgaga ccaagagcgt gatagagggg
    78481 cgccatccag ccggcgccaa ctcgcgccaa ataatcgtta acggtaatca aatgtacgcc
    78541 ctcgccgctt aaagcgttga gatataaagg tagtgtcgcc actaaagttt taccttcacc
    78601 ggttttcatt tccgcaatct ggccgcgatg caaaacaata ccgccaatta actgcacgtc
    78661 ataatgcctc tgaccgagaa ttcttttaga agtttcgcgc atattggcaa aagcttccgg
    78721 caaaatttca tccagactaa taccattttt aagcctatct ttaaattcta ggctcttatt
    78781 taatagctct tcattactta aggcctgaaa agacggctcc aacgcgttaa tcttttctac
    78841 ggtttttcgc aattctttaa taattttttc attcggatct ccgaaaattt tgtttaacaa
    78901 atccatataa tatttatatt aagcgcccta atgacaaaaa ttacgcccaa agcgctattt
    78961 tcagtataat ctgctttgcg tatttaataa atattgactt atatataata atgtgtttaa
    79021 aaaccccgtc acattaatta aagccacggg tttatttatt attaacagca tcaagcctgg
    79081 caagattggc agttctgcaa gtccttcaag cgcccgaact gctgggcggc attgacgcta
    79141 tgctgataat agagagtctt gatgccgagc tgccaagcgt acatatataa agcattaact
    79201 tctttaacag ggacagcggg gttgatggct aaatttaatg attgcccctg atcaatataa
    79261 gtttggcgcg ccgccgcttg ttcgatgact attttctgat tgatttcttg gaaagtcttg
    79321 aaaatttctt tctcttcggg tgacaagaat tttaaagact gaaccgagcc gtcattgtca
    79381 cggatgctat gccagacttc tttagaattt tgcccctttt cttccaataa tttttccaac
    79441 tgctggttct taatcgtcac tttcatctta tcaatatcct tcacgtaaca atttgaccaa
    79501 atcggctcaa tactttgaga aacctgcccc aaaataaaag cggaagaagt ggtaggggcg
    79561 atggccatta aagtcgtatt acggcgacca taacctttaa gaatagacgg ttcgccgtat
    79621 tcaagcgcca tcttttttga agccgctatc gcttgctcac gaatatcctt aaatatggcg
    79681 acattgagac gagcactttc ttttccctcc caaggcaaca gcttgctctg caaaagcgaa
    79741 tgccatccta aaacacctaa gcctaaagcc cgattggcca ccgcgaaatt atacgccctt
    79801 tccataaaaa ggaaagattg gcgcttacgc gcatcatcgc tgtcacgcaa agcttccaga
    79861 cggctaataa aatcactcat cactgcatcc aaaaaatata ctaaagtttc cacggcatcc
    79921 gtgtctttcc attcatcata atacaacagg tttaaagaag acaagtcgca aacaaatgac
    79981 cattcttcat ttgagggtaa taatatttcc gagcaaagat tgctggcctt aattttcaag
    80041 cccttatctt tataaacatc gaccgtatta ttgttaaccg tatcggtaaa gaaaatatag
    80101 gggtagccca tctgactgcg tcgctgtaaa actttagccc acaaagctct tttgtcggca
    80161 tcacccgccg ccattgcctt catccaggca tcagtcacag tcacaccgta agtcatctcc
    80221 tgaataggat tgccttcggt accgatatta agaaattcat cggcatcggg atgctccagg
    80281 ggtagataag gagcgaagtg cccccttctc accgaaccct gactaaccac attaaccaaa
    80341 gtttcaaaca gctgcatgaa gtggaccgct ccggaagaat ggccattatc ttttatcgcc
    80401 gcgccgcgat ggcgcaaatc gccgaaatag ccagctgtac cgccgccata tttactcatc
    80461 attcccactt cagcttgagt aaacagaatc tgccccatgt cgtcgcccaa atatgaagaa
    80521 aaacaactaa taggaaaacc tttggacaag ccgaaattag accaaacagg cgacgctaaa
    80581 gagtaataac cgcgcgacat gtaatcataa aacttatcgg caaaaccggg tttattcaaa
    80641 atcttttccg ccgttttggc gatatcttta attcgctgtt ccgcacttat ccccggatta
    80701 agatagccct ttgccaagaa agagcgagaa tattcattta accactgcat agtattttat
    80761 aaataattaa ttagaagacc aacatatcat cagcctaaaa gagatcgtcg ccggtaatac
    80821 tttggctgcg cttattataa ttaatcgaac gcttgacaaa gaaatcaccg tgcttggtcg
    80881 tcacgatttc ctcatcaaac cattctgtct gctccaataa cttttcgtcg accggaaaaa
    80941 ttctttccaa gccaatacta tccagtgagt tattcaaacg attcttaata aattcaataa
    81001 tgaccgtctt cggcaaaaat tccaattcac ccgcctctaa aatccaatcc aacacttttt
    81061 tttctgagtc aaaagcgacg tagccggctt gaatgatttt ttctttaaag ctttcatcaa
    81121 accattccgg atgttccgat ttaataagat taatgacttc aagaccgaac atgccgtgga
    81181 tttgttcctc cttagaagtc gcttccaccg cattggaaat acctttaaac agattcttat
    81241 gcttattaaa ggacattata atcaagaact gggagaacaa agaaacatgc tcgatgaaca
    81301 aagagaacaa aagaatagcc aaagtgtaat ctttattatc ctcacgctta gcgttgctta
    81361 tggaatattc aagatattcg acccgttcca ttaaaaccgg cattgtcttg atgcgggcaa
    81421 attcttcatt taatcctaga atttccaaaa gatgcgaata ggcatccata tgccgcactt
    81481 cactttcggc aaaagtaaag ccgacggcgg cgatttccgg ctttggcatc ttcttataaa
    81541 tatcgcccca gaacaccttg accgccacct ctatctgcgc gatcgctaac attgagcgct
    81601 gaatagcctg gcgttcaacc ggagtaactc tggtcttaaa atcctggata tcacttaaat
    81661 aattgaattc ggtgtgtatc caataagaat ggcgaatagc cctgacatat tcatataatt
    81721 ccgggtactc ataaggtttg agatttgtcc tctttttgaa aatatcccgg cgccgccgag
    81781 ccgcccgctt gtcgcgatag agaatgaaag cctgagccgc ttccttaaag gaactttccc
    81841 ttaaggcctg ttccgcccaa tcctgaattt tctcaacatt caaacgaaag gaaccgctgg
    81901 cgccggaaga ctccgcttca atttgggcct ggcgagccac cgtttccgcc aaggcggtgg
    81961 cgtcagcggc gcttccctga ccaacggcgg ctaaagcttt aaaaatggtg gcggtaattt
    82021 ttgcctgatc gaaagctaca atttttccgt ctcttttttg cacagaaaaa ttatattttc
    82081 tctggctaac attaatttta tccataaaaa aataaaaaat tataataaat aaaaatatca
    82141 atatatagag taataaataa tacttattat actatatatt gtgtcaggct gcaaccagaa
    82201 aaaaataagg gaaaaataaa aaaacagacg ccgccaagac gtccgcttcc tccttatccc
    82261 cagcaaaatt atgtttctat ttcccccgtt tctgatcacg tcgcttctcc cgataactaa
    82321 taataatttc ttctaagtga tccttgacct tatcaatcgc cttataaaga tcggcctcag
    82381 tcttctccac tctcaaaatt tcccgcggaa tctgcaaatt tatttccgcc cggaaaatct
    82441 cgcctttatt ctgtccgccg accgattttt ctagttccag gtcacaatta ataacctgga
    82501 tattgcccaa atatttttcc agcatatcca tcttagactg ggcatagtgg cgaatagctt
    82561 cggttaattc taaatgagtt gttttcaaat ttatcttcat atctttgtct tcttaatttt
    82621 aaacttaaaa taatcgccca atgtctttgt aggtcacaac gacaattaag gcaagcaaaa
    82681 gccagaaacc gatggtgtgt atccacgcct ctaccgactg ctttaccggt tttcccttga
    82741 atttttctat aacaacaaag agaatgcgtc caccatcaag agcgggaaat ggcaaaatat
    82801 ttatcaaggc caaattaagg gacaaaagcg ccataaattg caagagatag gtgaagccga
    82861 gactggcgac ttgcccggtt aaagtagcga tgccgactgg cccggttacc tgtccgctga
    82921 cactggcgcc gataaataaa tctctaatca aagtcgcaaa agcgacgata attgcccaaa
    82981 acatcaaacc cgtagcttta aaaccctccc agatcgcgat caagaagggg taacggactt
    83041 tgccggaagc ataaatagat acgccgattc ccggcttacc ggtgctggca ataactgttg
    83101 gcgtgatcgt cacctgtttt tcctcgtccc cgcttttaac taaaaaattg gtcgccactc
    83161 cattattctc cgctactaga gcttgtaaat tttcgctgct agaaaccgct tggccgttaa
    83221 ctgccataat cacgtcgccc gcttccaaac cggcatcagc ggccggagat tgcggcagag
    83281 tttccattac ccaaacctgc gcttgagtaa tacgcgccga agggccggct tcatccaaag
    83341 attgaggcaa gccgagcata taaccaagag acaacaagaa aaaggcgaga acgatattca
    83401 taataacgcc agccgccaaa attaaagcgc gcttccctaa agactgggcg gcaaaactgt
    83461 ctggctcctg cttaccctcg ccattttccc ccttaatctt aacaaaaccg ccaatcggca
    83521 accaatttaa agaataaacg gttcctttag gcttctgttc aagcggtaat tcatccaaag
    83581 atcggtttcc ccagaaatga cggcgctggc cctgctgatc tcgataaaaa ccgaaaagac
    83641 gcggcgggaa accaaaaccg aattcttccg ccttaacgcc taatttccga gctgtccaaa
    83701 aatgtcccaa ttcatggaca aaaactaaaa cagaaagaac taagataaaa attaaaactg
    83761 tcatcataat aaaagcttaa atttccataa tatctttctc ccggcgatcg cgcgcttctt
    83821 tgatttcctc gttcttcttg gccacaaatt catccaattc tttcatcgcc cgaaattttt
    83881 catcttctga tatttcttta tcggcttccg cctgctcaat cttagtcttc acttcttccc
    83941 tgagctggcg caaagaaatt ctggtctttt ccattttttc attcaatttt tttacgatat
    84001 cgcgccgatt ttcttcggtt aatgaaggta cggacaaacg aattttatcg ccttcattaa
    84061 ctacgccgaa accgagatcg gcttcaatga ttgctttctc cacggcttta gacacgcctt
    84121 tatcccaagg ggaaacagtg atactgcggg catcggccac ggcgatatta gccacggcgt
    84181 ttaaaggatt cataacgccg taagcttcaa cttgaagacc gtctaagacg gcgggattag
    84241 cccggccagt gcgcaaagtg gcgatatcgc gcttaaaaaa atcagaaaca gcttcgaatt
    84301 ccgcctgttt ggcgcttaaa aagttattca taaaattaaa ttgattattg taatttttta
    84361 gtgcctaaat aaataatatt ggggttactg cgatcaatta gtaaatccca gccagcgcga
    84421 gaggtactta gtttcttagt agtccaggta acgccgccgt cgctagaact gaaaaaagtg
    84481 gtgttagtca cgtaatagat ttcgtttgaa ttttgagggt tgacagcgat agcgttaatg
    84541 atagcgtctt tttccggagt aatcaacttg atgttttccc aggtcatgcc tttatcaggc
    84601 gaacggacaa ttgccttttc cgaagctaaa aataaagaac catcggcagc gcaagtaaca
    84661 atatcacgaa aattagaacc aaggttaaaa tcttttagga ctacgccgag gtcggtccag
    84721 ttttccacgg caaaattcct atcaatcgcg gccgaatcgg tggcatcagt cacggtatta
    84781 gaattaaagc tgtaaatatt gttcttggcc gtggccacaa agataagacg gctatcttga
    84841 ggagaaacaa ttaaacgggc gatactttca tttaagcgct gaatcgtccg ccaagaagaa
    84901 ccctgatcga tgcttttgat aatatccccc cgagaagtgc cgatataaat attattatta
    84961 ttgtaataat caaccgcgat cgtagtgacg ccgacgccgg ggttgttgtc ataataagtc
    85021 tgctgccaag agcgcccaca atcattggag cgataaagac ggttggtaat cgccgcatac
    85081 aatgtacatt ttgatttagg atcaactttt acatcattaa tcgtcccctg ccccaaactt
    85141 ttaacttcat tccagccatt attgatattg taggtataat acaaaccttt accgacagta
    85201 ccaaaataga ccgcgttgct atcctgcgga tcggccgtca agacgctggt atcaacgcct
    85261 cctaaactat agggctggcc ggtaatagtg ggcacgagcg ccatctgctt ccaagttttg
    85321 cccttatcca cgctcaacca gacgccgcca tcggtattag aactagtgga gataccatta
    85381 ggaccgaggg acagagtgca agcggaagtt aagacagtaa ccgctataag aaggataaag
    85441 atttttagat aacgcataaa attaaaaata aatagctaaa ctttctaaaa tattttgggg
    85501 actgaccaag ccctgcagat aaagtcgcgc tttagtcgtc tgtcttagcc ggtcaataac
    85561 cacttcggct gatagattaa aatctttttt ccaagaatct aaaagagacg gataacgcag
    85621 atattcctcc tggcctaaag aaaccagcaa agcatcgcgc cataaacttt cccagatatc
    85681 cagccaccga gaggcctctt ccgcactcag cttatcgccg ccgctttcct gattaataaa
    85741 acccaaacga aaggctaaat ctgacttgag aaaagtaaaa agctggccaa caagctgaaa
    85801 ataatcttga tacgaatctt gattttccgc ccaacgcaaa gcctgcaagg gtctgcctaa
    85861 agaggcggcc gccagctctt tggcttttga gcgcttaata tcaagactag ccagaaggta
    85921 atcgtaaaca gcggcgcttt tgaccggctg aaaataaaga acttggctac gggaaacaat
    85981 ggtggctaaa agcgcctcaa gtttttcggt taataaaata atcaccactt gcgctcgcgg
    86041 ctcttccagt gtcttcaata aagcgctttg cgattcagat gataaagtct ccgcctcctt
    86101 aataatgcct attttatagg aattcataaa agaactgagt ccgagacgct taataaaatt
    86161 cctagtcgct tccacgccta tcacttgttt atcatcatcc ttttctaaaa tataaagatc
    86221 actgtttaag ctttcaaaat taccgctgcc ggcggcgcct cggtccgatt gccataagtt
    86281 gcgggcaaaa gccagagcca ggctggattt gcctaaatct tttggtccag caaaaatata
    86341 agtttgtgct aatttaccag aaactaagct tttttctaaa aaagcgacga ccttgtcttg
    86401 gccgattggc ggccagtcaa atcgattttt tatatcagcc ataagattat tgtccaatac
    86461 taatctgatt taaatcctga ttgctgattc ctaaactcaa ataacgcata acctgaaaag
    86521 ctaaatcgcc attgcccaga taataacgct tgccatcttg aggattgaca taccaagctt
    86581 cgccattttt ctgcacctgt aacaagatct tacccttctg tttagcggca aaacttaaat
    86641 cataagacaa agaaccagga ccgttaggat tatagcctga acgaagttct tcgccgtcta
    86701 aataaccgtc accgtcgctg tcgctgttat taggattact accgatagct acctcaagcc
    86761 cgtccggcaa aagatcgccg tcgctatcat tgccggaaaa acgactgtca acgccaattg
    86821 gaatttttct taaatcagcg tcagtgatgc cgacgccact ttgacgcatt atttggtagg
    86881 cagcggcgcc attagccaaa taataacgct tggcatcctt aggattcaaa taccaagcct
    86941 gccccttatc ttccacctgt aataaaatat agccagcgag acgcttaaca agagaaccgg
    87001 aggcggtggc ggaagaactc gagtttgggg ttgaagcgct tattgccacc gacaaagccg
    87061 gacgactgag agtaaatttc tgccccgctt caatttgctg ggctttttcc atttttaaat
    87121 ttaatttatt gattttgccg ttctgcgccc agaaagcctg gccgtattca attttatttt
    87181 tgtcaatata cgataataaa taggggccat cagccaaaag cgcctcaccc tgcttattat
    87241 ttgccaactt aatactgccg acttctttgt ctttatagaa ataaccgccg ctttcataca
    87301 aggaatagac ctttaaaaca gtattagcgg gcatcaattc ccctaaaccg ctagctaaag
    87361 taacgcgagt aacattgagg ctcaaattaa atctatttgc ttgctggtct ttaataacgg
    87421 cattccagaa aatattattg cgcttgaagg aatcggtgac cgctaaagca taagtaccgg
    87481 ccggatattc taaatgcaga ttaccggaac tatccgtctt accgctagtt aataaatccc
    87541 ccagagtata agtagagccg ttaaccgtct gggcgtaaag cttgacttca gtattggcgg
    87601 ccgcctgacc ggaagcagat ttgacactta aagataaatc actaaaaaca tattcaaagg
    87661 tagtgttctt attagcctcg accgcgatat tataaacatc gaacacactt ttattgaccg
    87721 tcgcggaaaa aatatacaag ccgcgcttat tttgatcgaa aggatgagcg ggcgccacat
    87781 aaatggtcgc gaccccattg ctgcccgtct tcagagtggc aattaatttc tttgcatctt
    87841 taactggacg attatcgaca tcgaaagatt cttcatataa agagaaagaa aaatctttct
    87901 tcagattgcc cctggcatcg cgcaaaacaa ctttcaaata aggtaagctc ttggtgattg
    87961 tccggtcata gccgcagacg aaacgagcgg catcaaaaaa ccaaaactca cctaaatcac
    88021 tctttttatc ataagcttta agggcatagc ttttgcgcgg atccggctta aaggtgagct
    88081 cggctttgcc agcgctattg gtcgtcccgc ccccgatttt cttgccggcg atagggcggg
    88141 caaaaacatc gacctcctgt tcataaactt catatttaaa tccacttaaa gcttcgccgc
    88201 ccatacccat caaattgata gttaatttga cgttatccgc gcaggtctcc ttacccgatt
    88261 gccaagaagt acccacctgc caatcatact cgttcaattg tccatctaaa atttctacat
    88321 cccatagata atcatactgg ccgttactac ccttcacccc taaaacataa actccggccg
    88381 gatattcaaa aataccgtag ccatcatcgt tggtttggaa ttccccgact ttatcgcctt
    88441 ggacttcatc attattgcca tcaattccct gttcgtatac ttcaaccttg gtcttcccag
    88501 ggaagagagc gccggtggct gtccttaaag tcaccttgac ggcgctggaa taatactcgg
    88561 aattagtaag cagttcgtcc ttcactttga tgccataaag gtcaaattta tggccattac
    88621 gtgttaattc caacacataa tcgtcgcttc gactgccatc aagactgcgc acgcttcctt
    88681 gaggcacgta aatacgggcg ccgccgctcg taccggtatt taaagtcgcc accaaatctt
    88741 tggtttgctt gatgggattg ccgtcaagat cataattctg agtgtaaaca ttaaaattgg
    88801 tgttatacaa caaattaccg ttgtaatcgc gtaagacaaa attaatgccg cttaaaattt
    88861 tttcaagact aatctgctgg ccacagctta atttcaagtc gtaataccaa taactggtaa
    88921 aatccttgtt gattgcttga attttaagag cataaacatc gctctcggca ttgctattgc
    88981 ggaattttaa agtggcgata ccggtactgg cattggcgct ggcgctggcc accttggccc
    89041 ccggcttggg tttgccgttg gcatcaatca cctgataata gagttcggct ttgatgccgg
    89101 aaatataaga gccgcctggg tcgcgggcca caacatcaag gagagtatcg gcattacaga
    89161 cagccgcgga agcaggcaag ggagataccc ccaaaaacag gaaagctaac gccgccaccg
    89221 aagccaaaaa atatgattta gcaagaaaat tcataaagat aaatttaaaa tctaaactat
    89281 tttgaaatct tgattctcct ttttaacgga aaatcttttt taattatatc atgtcttcct
    89341 cctagagcca aataaaaaac aggcttttta cctgtttttt tctaattatg gcaagagaaa
    89401 actttcgtta atattcctaa tagagggcgc caatgagaga ttatagtttg ttcctaccgc
    89461 cgtcagactg tttaattcca aatcaacgct ccggccactc tggtaatccg aattcaaatt
    89521 ggcgctaacg ctcaattcaa ggctgtctcc agccaacaag cgtaaatagt taacatcaaa
    89581 gactatctgg ccatcctcca tgctcctggc ggtagcaact agttgattac cacgccttaa
    89641 tttaaataat tctaaatcat tggaggaggc agaaccatta tttctaaaaa caagttgtct
    89701 gatataaaca tcttcattgc cgctagtttc ggatatccga aatttaccaa tatttttaac
    89761 gctgccatta gcgcccaagg gctgttcttc tgctaccacg gttcctaata tatgaccggc
    89821 atcaaccatc ttaaattctg tcccgataag aggaaaactt ccggaaagcg ccaaattgct
    89881 ggttaccgcc tgaggatttt ctaattgcaa gcgaatggtc tgattattaa cattatctaa
    89941 ataattcaaa tctaataaca cttttaactt aatcgtatta ccggcgggca cataaaacga
    90001 attctgacga aaacggaagg tcagctgacg gtcatgatag ctactgctgc cttcaatttt
    90061 tttaccggtg tcagcgttaa ccaaactaac gctgtcaatt aaagagtcgg aataaatacc
    90121 ggataattta aaggtgatat tttgaaataa ggccgcttca tcgccagccg taaaatataa
    90181 aagcatcacc tgattatcgt gagtaccagc gaccaaatga ttgccagatt gaggattatc
    90241 taacaaggct tgataaacac tcaaaccgga gttttttgcc aaaccggcgg cctcataaga
    90301 atcttcagaa gcgtactgcg ataactcaaa atcactgaca gtaatcggca agacattttc
    90361 taaattatag accatgatgt cttgtaaagt attgatcttc accttctcgc tgccgcggat
    90421 ataatataaa acatcgctct cagccgtctt aatcagtcgc gcttccggcc atttagccag
    90481 ctcttccgga ctgatgattt ttatatccga ccaatcattg ccataatcca aaaacaccgc
    90541 ctcgttaata taggctttcc tttgatgggt agcatgatca agataataga cagtgctttc
    90601 acccggcact ttcacttggt taatttctgg acttaaagct ttaacttggc tgatattaaa
    90661 gaaaaaaatt gaaaaaataa tgagcaagag cccagaaaat tgataaatat aggttttttt
    90721 attaaattta agcataaaaa tctcgtaata aagtcatttt ttacctaaac aaattatatc
    90781 acttttataa attagtgtca a
//
LOCUS       DAOQSA010000002        50581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_310_length_50581_cov_9.315600, whole genome shotgun sequence.
ACCESSION   DAOQSA010000002 DAOQSA010000000
VERSION     DAOQSA010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 50581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50581
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_310_length_50581_cov_9.315600"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..7000)
                     /locus_tag="PKK65_00495"
     CDS             complement(<1..7000)
                     /locus_tag="PKK65_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81202.1"
                     /translation="MKKHSNLFIKRHKFGVAVILLLFSISFLLMPLKPVQAQAVPGTD
                     VNQAVNNILTPIWAFLKKAYEKGGAAAFQKTVRTALNKIAYDTATWLGSGKEGQKPMF
                     VTQGWGSYLAQIGDEAAGQFIEETVNNWNNSKLKEQNEAQCEEALNECENNCSFSIDE
                     LSNDEFNDCLNDCLNTFNACRGKNNASNVYTGADGVTTTRTAKTVAVCQPSISAKLTI
                     AMGLVDYNRPAAPNCTASALVQNWDNYIDRMIAFKEDDFLDKFKGIFNPVSNDLGIYL
                     TLQSDMTKKIEEKKEDSKTKLIADGGWLDMRDIAGNLVGLPGEAETKKEMSEQNYISN
                     MATFSGDALIDAANVFLNQLALTAFNKFMSNLGKISSDNNSAAISNPATDPNVVYGEV
                     SVRESAASIIEPDFTVRADYDILSSLTMCRDRNNPGPTECVIDDKLMQGITEKKTVAE
                     AVKDGYLHKEWLFTSDYREGAYNLRNLQIMRKYRIIPIGWEAAAAFGKQATLIDLMSC
                     FSATDAYNEYTSNFDVRNQGWCRGLVDPNWVLKAPLNYCAKQGYSAQILDIAVVPSAA
                     SDGTIADDVQITRADDYCADDKTCIKERADGSCEAYGYCQSERRIWSFASKSCKPIDN
                     TCSAFLNSSSGQRVAYLENTLDYGDCSADSAGCKKYSFNGSYNNENGQIVWSDNPLAT
                     AYFNNKLFSCSSSQEGCSEVMRIKPGWGSNLIMGADFAEDNIGDELVNGKINNYWPVW
                     STGNKAAEIIDVSTIDSNSSGKAILVESVGNSTGTPIGIFSDNAVSLIPSNLNILFDE
                     TYTLSADVYLLAGDKIDLVLGGDYATTVSTTDHDVWRHLSVTRNLAEKPLSEMSFSVV
                     AYSSTSETRFLLRNLKLEMSPWDSGFSAYGSYNVYEKLIPPYLAPVCYENANVGNPDY
                     RLKADAPEICKNFARQCNRDEVGCERYTEKSTAFSVAAQARSSDYCDASCDGYDLYVA
                     RANYFYSPYAEKIIPKNSRACSAEAVGCSEFTNLDAAASGGEGKEYYTQLKQCIKPGA
                     SCGDFYTWVGTEESGYQLKALVLKKDANGSPAVTADDSAECNETIFNLPPSDPRFNPD
                     CRQFYNKAGAISYHLSAYTITCSENCHPYRLSENNIDTTLTQAQCSGSDRFWNIASSV
                     CYVCKNGGTWSTQHQACIYQAIPNEGVKCAASQNGCREYNGSLGNNTKLVDADSFENN
                     LEGWEGQCGDTVLSSQVVSTNNGKSLFYDSGANATGSIQSTSCDNPTQLSWFDRFFGK
                     ANAALPSHVRKIIGKQAVQGQSYSLKFTAAASVETTLSFGFRNSNGEITYFNASDSNP
                     TGSISISGDSEWRSFELNLPELNHVVDANESLIIVANHDFYLDNLILTAISDRYYLIK
                     NSSQVPDVCYYDMLDNYRGANYNLGCSLYNDRTGNSHYLRQFSRLCQDSAVGCELMVN
                     TANYNSYKPGIWKDGNGNGQCDANEPECVSVAGDRFFYAIYDSSYLCNRADSGCSRLG
                     EAVSTGTTTDYSDVFKLNQPDNYNNSLCNASEAGCEAWQYIDGSGVSYFKNPGNNACV
                     YRSSINPDKPGKAWYKVPVMRCDLNNNGKIDATESQASPLCQSADDCSADRACLVDNN
                     DYECPVSYNRTIGFGGGGNQVPVPSEAAGLCEIAASGCTEYIDPVSSFSPNLIFNAAL
                     DNNCNGWQSSSTDNSCSYPLSQDIVLEPNKLYIFALDSYSSNPIDYSVSLTFPAGVSL
                     LQEDNTLSTSASSTLTIPANQPSKRFIFYSRGNTAATVSGASSGYFVSVKEAIVDYQF
                     KQNIDKKSCNGLVNFDDGCVLFNERSVNGGEGLLSLSGGWDAFASTNGQAPVNCTSGN
                     CSANTLIKVRPDRVCATWLDCLTYVVDPVTNQRTCYALGECNNLNDNNECANFVNSGK
                     GTILNGESDIAVMSKIDGYSVLNQYYFADMKEVGLNTEAHYNFEEGSPSLTCRYVNPP
                     HNDCSFDNGIVADSIINNPKNAPTDYPGEGRAYLKVMSSQQISPHAENAPISIQPNQD
                     YYVNYLLNTKGSGLNGRVWIYDAIDNTLIASSVANSPNGWERQVFKISKNDVGTTTSI
                     KIYVGSDSNVKEERYVYFDDINIEPVLQVGKDKYVAKECRLYPTQDATSCTSKNSQVV
                     SDGLVGYCLQHDLANKNVCLLWYPIDEISANAKSARSTLGYQGAYPLNYCTQANGNFV
                     LLEKRVGFKIEDLKGGESVEESKCYKSTSNKKPRPCVTWNNPLDDDNYEMWISEEKGG
                     YIKKICVPKQDKLLIKTEEITLDNQMGGCEGETYYIGWGEYDGFSSFSYTVGHEHGCD
                     EGEGCSNMNEATVADPPVRVYDYNYPT"
     gene            complement(7323..7448)
                     /locus_tag="PKK65_00500"
                     /pseudo
     CDS             complement(7323..7448)
                     /locus_tag="PKK65_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IclR-like transcriptional regulator"
     gene            complement(7733..8587)
                     /gene="rsmA"
                     /locus_tag="PKK65_00505"
     CDS             complement(7733..8587)
                     /gene="rsmA"
                     /locus_tag="PKK65_00505"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNU81203.1"
                     /translation="MDSLAARTKELCRLYEIKPARSKGQNFLINDGIYQAMIAAADIN
                     KKDTILEVGPGLGFLTAALSKKAKRVVAVELDDKLAALLQIALDSQGVDNVEIVHQDI
                     LRFNPDKYFDSGENYRVVANLPYNITSFFLRTFLSSPRPPRSLVLMLQKEVAERICAQ
                     PPKMSLLSLSVQYYGHPQIIRTVPASDFWPQPAVDSALLRFDYLRNKTTKESLEFDKK
                     FFRLARIGFSAKRKMLKNNLSGGLKLEPGVITGALIKVGLQVKARAEDLSVEQWRLLV
                     AAIPDFVL"
     gene            complement(8592..11024)
                     /gene="leuS"
                     /locus_tag="PKK65_00510"
     CDS             complement(8592..11024)
                     /gene="leuS"
                     /locus_tag="PKK65_00510"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020156908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNU81204.1"
                     /translation="MYNHQAIEKKWQQRWRASKLYRTGEDRQKPKYYVLDMFPYPSGA
                     GLHVGHPKGYIATDIVARKRLMQGYNVLHPMGWDAFGLPAENYALKNKVHPRQATQKN
                     IATFKKQLELLGFSYDWEREINTTDPKYYRFTQWIFLQMWRRGLAYESYAPINWCPSC
                     QTGLANEDLEDGHCERCGSLVEQKPMRQWVLKITKYADRLLDDLDKLPDWEEAIKEMQ
                     RHWLGRSLGAEIDFQIFGSEEKITVFTTRIDTIFGCTYVVLAPENPLVMSLKPKINNW
                     LAVENYLQEVKDKTELQRTDLNKEKTGVCLEGVFAVNPFNQEKVLVYLADYVLNSYGT
                     GAVMAVPAHDERDWEFAQKYQLPIRQVIITNNRESLNLEQALITDGTLRDSGDFSGLS
                     SSEARIKMIDWLQKNNSGREKVNYKIKDWVYSRQRYWGEPIPLIHCDKCGVVPVPEEQ
                     LPVTLPEVESYAPTGTGESPLAAISDWVNVVCPHCGGPAKRETNTMPQWGGSCWYYLR
                     YIDPHNQQELVAKDLEKYWSPVDLYVGGAEHATRHLIYARFWHKFLYDIGVVNYEEPF
                     IKLQHVGLIMGEDGRKMSKRWGNVINPEEMAKRFGADALRLYEMFMGPFDQTVAWSTK
                     GLSGTRKFLEKVYNIVDSSNKLPVKEEKQILSLLHKTIKKVDEDIEAFRFNTAVSAFM
                     ILINSVTDYCQAHQAMPLSSENLGKLLQILAPFAPHLSEELWDKLGFTGSIFKSKWPA
                     YQEELIKDETVTYIVQVNGRLRARLTVLAGADEEEIVHLAISEGAVSKWLEGKDIKKR
                     IFVPGRLINFVV"
     gene            complement(11038..11796)
                     /locus_tag="PKK65_00515"
     CDS             complement(11038..11796)
                     /locus_tag="PKK65_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005815852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNU81205.1"
                     /translation="MKIISWNINGIRAALKKGLLDWLQQEKPDIICLQETKISDEARE
                     KIRLEFEGYNSFWHGAKRPGYSGTAVLVKQGSKVKVSNGFGPSKDDDEGRVQTIELSD
                     FYLLNVYFPNANAELTRLAYKLDFNRDLLAYINKLESNKPVIIAGDFNVAHQEIDLAR
                     PKENAGHAGFTTEERNWFKQLLSAGYLDSFRLLEPKKVQYSWWSFRAGARRKNVGWRI
                     DYFVISAKLRKKIKKAFILDQVLGSDHAPVGIEI"
     gene            11932..12684
                     /locus_tag="PKK65_00520"
     CDS             11932..12684
                     /locus_tag="PKK65_00520"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNU81206.1"
                     /translation="MSIDERIKYLEMKEKHKKKLRPWYKKPWGIIIIILLLGLIAAVS
                     ASAFYVANKVQEIRKGQSAEQQAAEIQKITAAIDGRGENYSLGPINAKVKIVIFSDYA
                     CPYCRQAATVVRNLAKKYGNQIRITFRDYPLHDNSVDLALAVNCAGSQNKFWEMNELL
                     FNNQDTLSSLQGPELNLKLKELANSLGLNADNFASCLDNKTYLYRLNDDFSDAELLGL
                     SGTPTWFINRYKITGFYPEENFIGIIDGLLAQ"
     gene            12692..13258
                     /locus_tag="PKK65_00525"
     CDS             12692..13258
                     /locus_tag="PKK65_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNU81207.1"
                     /translation="MIQIRIHGRGGQGVVTAAELLASAAFLDGLYAQAFPLFGVERSG
                     APVQSFVRLDKKPIITREQIYRPDILIIQDDSLLAVKEILVGIKPNSILIINSRTKKT
                     DLASKLKNKIKRHNIYPSPATEIALELLGKNLVNTVILGSFAQVTKLISLKSLLAAIT
                     DKFADKGKEIINKNKNAVLKSYQYEEKE"
     gene            13242..13520
                     /locus_tag="PKK65_00530"
     CDS             13242..13520
                     /locus_tag="PKK65_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014405441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNU81208.1"
                     /translation="MKKKNNPHRPFARPGSSLDNKTGAWRTYRPEIDKNICIGCSRCA
                     KLCPDACIFMKMDKNNKLKAEVDYDYCKGCGICASECPVKAIKMTKDY"
     gene            13567..14721
                     /gene="porA"
                     /locus_tag="PKK65_00535"
     CDS             13567..14721
                     /gene="porA"
                     /locus_tag="PKK65_00535"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HNU81209.1"
                     /translation="MVLNNKTKFLEGSRAISLTVSNISPEVVSAYPITPQTHIVEDLA
                     QLKAEGKADFEYLRAESEFAAASIIVGAAAAGSRVYSATSSQGLILMMEVLYNAAGLR
                     LPLVMTVANRAVGAPINIWNDHSDAMAMRDAGWMMLFAENHQEAVDLHVIAYKLAESY
                     NLPVAINVDGFILTHSYENVIIPDKKTIKKYLPTYKPAPGTYLNPQKPITLGGFFTPE
                     HYWREKKKLDEDLKKTGASLSRAYREWKKILPSPLSVKPIFDNGLIEYCGPKNPKTIL
                     ITMGSVAGTIKEVLKKEKNSGLLKIRSYRPFPAQDIKRIIKNCPRLAVLEKASSAGAY
                     GPLYSDVMSSLPQYQGLSSDHIIGLGGRDISTAIIKNIIKSAGKAGLKFW"
     assembly_gap    14803..14902
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14903..15879
                     /locus_tag="PKK65_00540"
     CDS             <14903..15879
                     /locus_tag="PKK65_00540"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HNU81210.1"
                     /translation="DIKDPLIFEPQIGLPGLVDKGQQITLSNKDTSYIAQMVKGFYNY
                     GIAIAGILAAIVLMAGGIIWLTSAGSSEKISQAKGLITGSLTGLLLVFGSWLILKTVN
                     PYLVDFKISKIKAIQPKTFCCHPTRGNVITITDKNGNESCPDAESKKCENNTRCENVG
                     TREDNKFGCINKENFYCCQYESSKGYTYCKPVSVHEVIQGANCGTIAPTDYTWEKTYN
                     YYCQERRIGSTNINCTNPCTGKNNGDYCYGKDIEDSCRCYNEMEWHGQTAYKEGEPCG
                     MKDGSICTSKGTGGQIFPRCTEKGWSHDNNAWRRRDCGEGLYCCHPDY"
     gene            15881..16747
                     /locus_tag="PKK65_00545"
     CDS             15881..16747
                     /locus_tag="PKK65_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013719172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNU81211.1"
                     /translation="MDKKKNKITPGHNACAGCGQLSAVQAVMRALSDDTILVNATGCL
                     EVTTTAHPQSSWRLPWMHSLFENSAAVASGIKATLKHKKKKTKVVIQAGDGGTYDIGI
                     GLISGMWERNEEIVYICYDTESYSNTGIQASGSTPWGANTVTTPAGKNSLGSEQNKKE
                     MLAIALAHGLKYVAQSTSAFPDDITNKVKHALETNGPSYIQILTPCVPGWKIKTDEAI
                     RAARLAVQSGLYPLLEYKDGKIIDSSINDNFKASPVDEYLRIQGRFRHLQPEEIIKIQ
                     NLANQNIARYHL"
     gene            16744..18042
                     /locus_tag="PKK65_00550"
     CDS             16744..18042
                     /locus_tag="PKK65_00550"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNU81212.1"
                     /translation="MIKKTIFIYLFLLLFLPNIAVASETTRENCSLETIPKGQTSAGT
                     CLLKSKQEAFFPNCGDTLPTGKVCCCNPKTSDYTCRWKTIMYVTDTSSLAPGGGWYGD
                     CDADETKGIDCDPSTMPETNYKTRVICCCPEVTPVYKKADFIIPEFQIPIDTSTLTEV
                     YCTGPDNSGQCEIPWIAEYIQIIYQYGLGVGGILAAIVLMAGGVLWLVSAGDVSKISQ
                     AKDLIIGSITGLIILFGTYLILSQINPELTTLKSISVKMIDRIIIQPIKNGSDSTENI
                     SDNCADVNSLVTITDIVESNAETPMLDKIGKEGLIKAVEEAKKQGVELYVTSAFRTAA
                     HQQRLWDDAMAKYKDANLAAKYVARPGSCGGHRSGRAIDVCIKDTASCQKMGSAAVAN
                     YSDEDVVKLKAIMKAAGWQRYRGEWWHFQYQEPMKNADNS"
     gene            complement(18126..18527)
                     /locus_tag="PKK65_00555"
     CDS             complement(18126..18527)
                     /locus_tag="PKK65_00555"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HNU81213.1"
                     /translation="MKIIIHSDGGARGNPGPAGIGVLLTNEQGVSVAEISNFIGRATN
                     NQAEYQALLAGLEKAKSLGAEEVVCRLDSELVVKQLNHEYKVKNKDLAPLFLKVHNLI
                     TSFKKISFQHVPREMNKEADKLANEAMDRGK"
     gene            complement(18563..20386)
                     /gene="recJ"
                     /locus_tag="PKK65_00560"
     CDS             complement(18563..20386)
                     /gene="recJ"
                     /locus_tag="PKK65_00560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNU81214.1"
                     /translation="MQKYWQINPVAPPEFFSSLPEIEPVIAQILWNRGLRSATVARAF
                     LSDALEPELSLDLTGNKDLVFYNPFLFRDMQAAVDIIVKHIKARNKIVVYGDYDADGV
                     TAAVVLSEALKTLQAMVDVYLPDRVSEGYGLNKSALDQIKEQGVSLVITVDNGIRNKD
                     EVDYAHRLGLEVIITDHHVLPENRSDLPACPVIDPADAEDKYPWPFLAGVGVAFKLVC
                     ALLYQATLPAKQKFLIAERSLDLVAVGTIADMVSLLGENRFLVKRGLSILNQNRRLGL
                     KALFQTAKIALDRPLEEWNVGWQLGPRLNAASRLGHANSAFALISAEDEVEARNLAQE
                     LNQRNLNRQQITEEIIKQMEAQIDKDNLPPLIIGVAQDGQSWNEGIVGLIAGKICDKY
                     HRPTLVVARIIEEEERNQADTGLKAAQLSFKGSGRSIEGFNLIAAIEECSEFLDKYGG
                     HPMACGFSIENVENLNGFRDKLLVIAKKKLTPEILVPKLSLDSALSAREITLSLADSL
                     TKLAPFGQNNPQPKFTSANLRVDDIVWMGKDRQHIKLRLAAANSQDIFSVGAIAFNAF
                     DTFKDLQIGDIIDLAYYLEINEFNGRRELQLKIIDWRKSTD"
     gene            complement(20547..22346)
                     /gene="lepA"
                     /locus_tag="PKK65_00565"
     CDS             complement(20547..22346)
                     /gene="lepA"
                     /locus_tag="PKK65_00565"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017319863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNU81215.1"
                     /translation="MNDNLHKIRNFAILAHIDHGKSTLADRMLEITNTVDKRKMKNQI
                     LDGMDIERERGITIKLQPVTMTYQGYQLNLIDTPGHIDFSYEVSRSLAAVETAILLVD
                     ATQGIQAQTLANLYLALEQDLTIIPVVNKIDLPAANVFKTKTEISRLLGGAESEILCA
                     SGKTGEGVEEILGAVITRGLPPRASSASVGRALIFDSKYDEYKGVVAFIRVFDGEIKK
                     GDPIVLSATKAKSEALDVGIFKPEYFSTGSLQAGSIGYVVTGFKQVGDCRVGDTLASG
                     REVVPALAGYKEVKPMVFAGVFPREGNDWEDLREAMEKLKLNDAALSYEPERSDALGF
                     GFRCGFLGLLHLEIFQERLRREYNLDLIVTVPSVAYRVLKKSGEDIIVRTPQCLPDPS
                     EIEKIEEPWVKLDIITPQAYLGNIMSLSRDKRGIYKNTEYISTGSDEARAILHYDMPL
                     SAILTDFYDKIKSVSAGYASLNYDYLDYRPTDVVKLDIMVAEEPVESLASIVYRDEAY
                     RQGKEIVEILKETLPRQMFVLKIQAVVGGKVVAAERLSARRKDVTAKLYGGDVTRKRK
                     LLEKQKKGKKKMMAAGKGSVVIPSDVFVKILKR"
     gene            complement(22446..24077)
                     /gene="murJ"
                     /locus_tag="PKK65_00570"
     CDS             complement(22446..24077)
                     /gene="murJ"
                     /locus_tag="PKK65_00570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNU81216.1"
                     /translation="MLSLRKLIGRPINNITAAAALVALFSVASRFLGVVRDHILAGSF
                     GAGAELDIYYAAFRVPDFIYNLIVLGALSAGFIPVFSEMIKRSEIWPGEKEKLVRLAN
                     NVFNSLLLLIILLSVFGVIFAAPVTAWITPGFSAEAKAQTAHLTRIMFLSPLFLGMSG
                     VLGGILQSYKRFFVYSLSPVFYNLGIIFGALVLVDFWGIDGLAWGVVLGAVLHFAVQL
                     PTVWSLGFHYRFLLDIRDRGLRQIAMMMGPRTLSLAVSQINLLAITALASLLDSGSLA
                     VFNLANNLQSFPLGVFGISFAVAAFPFLSENAYKIEELKVRFSATIRQILFFIIPASV
                     LLITLRAQLIRLVLGSGAFNWQDTILTMNTLGFFSLSLFAQASLPLLVRVFYARRDSL
                     TPFYLGLFSVVLNIVCAWYLHPYLGVAGLALAFSIASIVNFLSLWIVLYFRVGSLDLD
                     KILVSVLKFSVAAIGAGLVIQILKIAVWPFIDMTTFVGVFIQFSVAASGGVIAYICLC
                     HLFRSEELLSAIAIFKKRLSVKKVKLADQGEARGL"
     gene            complement(24099..25679)
                     /locus_tag="PKK65_00575"
     CDS             complement(24099..25679)
                     /locus_tag="PKK65_00575"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNU81217.1"
                     /translation="MRFFFWEKIKSSYHWRSWHAYLFLILAALIVYGQSLFFDYSYFD
                     DQQLILEHADILERANPAEIFLNDVFFSAAKYYYRPLLTWSLVCDWHWGGGTVFAFHL
                     SNIIFHAVAACLLFYLLRLIDIRGKTALFLSLLFTIHPVLSPAVAWIPGRNDVLVAVF
                     IWATLIFWSRWLRREKIGDLVALWLFFLSALLSKETAILLPLFALAWMFVFEYRYFTW
                     FKLLLASAGALVSMFLWALLRLVALGGTGSGLAWQSLGDNLWSPLIFLGKVVLPVNLS
                     VYPVPADSPWIYGIISLLILMLLLFYSRPWRGKRLLFGFAWFWLFLVLGSIRPDSDVF
                     RNFMEHRLYVPMFGLLIILAETEKFWQGLPLRRRRYVLATILVVLATINLFHSRSFRD
                     RLSFWEQAASSSPHSALAQRNLGAMYYLEGDLEKAEGRFRQSLFLNSSEPMAHNNLAA
                     IYIDRGDFGRAEIELKKELAINPQYDLALYNLGRVYYQYRRYQEAAALWNEALRLNPR
                     LDSAAQALTALQSEAEVK"
     gene            complement(25683..26396)
                     /locus_tag="PKK65_00580"
     CDS             complement(25683..26396)
                     /locus_tag="PKK65_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013410178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNU81218.1"
                     /translation="MYQGKKIIVVLPAYNAAATLEQTVADLPGVVDEIILVDDGSSDD
                     TIKLAQRLGLFVFAHQRNRGYGANQKSCYRLALERGADIVVMVHPDYQYDPRLVSYFA
                     SFIADDYFDVMLGSRVRSRQETLRGGMPIYKYYANRFLTFVENLATGQNLSEWHTGMR
                     AYSREVLSSLDFSKTSDDFIFDTQVLLQIVQRKWRIGEIPVPVRYFSEASSINWRRGL
                     RYGVGTLFLAIFYFFGYRP"
     gene            complement(26578..28056)
                     /locus_tag="PKK65_00585"
     CDS             complement(26578..28056)
                     /locus_tag="PKK65_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81219.1"
                     /translation="MKMPLNKKIRFFWLILLFFIIIFLAYQFVSPSGDWSCTHSFSSF
                     QASSCLGDPTPKERVIKTQGGPLLILGEPVYFSVFSPRPFSHITIKITYRPHLSSSTP
                     IFEAGFLADKKLWRYRLQPVYNLWLEQGLSSWTQLRSGNLSLFQRKEKFSSLSEFLSV
                     WRSQGSSFCSQPRCLAVYNLSLEEAPPALDLRSLSPVSENSFFPYPLRGEHQFYIFLK
                     NKGLSLSGNLLDLNENKDIDDAEIVVFSGRNQVDLLSLPDRRPQKEESGDIGIIQSVK
                     IVNSDLPSGLYRLEFRAGDDLVLSDLSVNSAYLSIINKIWPFTEEAVSLVTDAPYLQV
                     KALSPAALQTLDFGGRHLELTEIYKQYEIKSKRLGGENIRLSTGSLILANNGMFAASS
                     SALINPDYPRLDRFSSLNEQLDFVLADYRPAIIEDEGWLSSTLEFDASDLYRERGKYS
                     LILSIPGLLAEGGGGGLVEIKSINVEFSGPSLEAKIKDWLNL"
     gene            complement(28202..32194)
                     /gene="rpoC"
                     /locus_tag="PKK65_00590"
     CDS             complement(28202..32194)
                     /gene="rpoC"
                     /locus_tag="PKK65_00590"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNU81220.1"
                     /translation="MLNPIKTSDFDALRLKLASPEEILSWSHGEVVRPETINYRTQKP
                     EKDGLFCEKIFGPTKDWECACGKYKKIRYKGIVCDKCGVEVTRSVVRRERMGHINLQI
                     PCTHIWFLRGISSKIGLLLNLGIQSLEKVIYFASFIVTDVDEGLKEAALEQIKNEHKS
                     KRKSIEAELVQQIKRIQDSRADIIKEGKAKAEVDASIANQIKVATAERDEKLKRLEED
                     FIQVSKELKELKPLLILSEQQYQNLSLKFGHLFEAGIGAEAIRLLLSRIDLAKSIEKI
                     EEKLKDAVDSKRDKLTKRLKLLKSFRNNNLRPEWMIITVLPVVPPDLRPMVALDGGRF
                     AASDLNDLYRRVINRNNRLKQLIDLNAPEVICRNEKRMLQEAVDALIDNSARHTKTVT
                     ASTGQKRQLKSLADGLKGKQGRFRQNLLGKRVDYSGRSVIVVNPKLNLDQCGLPKIMA
                     LELFKPFIISQLIKREIVHNVRSASRYIEAGHDEVFDILEETVKEAYVLLNRAPTLHR
                     LGIQAFRPVLIEGKAIQIHPLVCTAFNADFDGDQMAVHVPLTKEAKQEAADIMLSSHN
                     LLKPATGDPIVAPAQDIVWGTYYITLEDPAYKDKKDQELKYFYNDDDAILAYDHNFVS
                     LNEPVIAKINGQRLRTTVGRMIFNLILPEKLRYINEVVGKGKLKDLIRECLRLYTEDE
                     TVKFIDNLKNKSFSFITKSGLSWGFGDLPYLPEKDRLIAEANDKVDVIQEQYEDGLLT
                     ESERYTKVIEVWANTKDQIADICKEGLVNILPVFSMIDSGARGSWAQPVQILGMKGLV
                     TSPSGAIIELPVKGNFKEGFGVLEYFISTHGVRKGLSDTALRTANAGYLTRRLIDVAQ
                     DVIVTEDDCGDKKGVCYIKEETEKTGEDFYKKIVGRYLAQDLLDDKNKVLLKAGSLIN
                     EDVSKDLKAKGVSALNLRSVLSCHLHKGVCAKCYGWDLAYNKEVKLGAAVGIIAAQSI
                     GEPGTQLTMRTFHTGGVAGQDDITQGLPRVEEIFEARSPKKKALISDVAGRVKIEFAQ
                     KTIKDQDGKDIVITNPQAKILSIIYRGKEQEKYYFSEKINELKLASGLTVKDKKKLIP
                     EISVKDGDKVAKGVEIFKVGGESVKAKSAGVVSINDKYVSITKDVDKVKEFSILKGTM
                     MMVKDGDEVEKGTQLTEGSLDLRELYKLKGELETQKYIIREIQYVYSSQGQPLNDKHV
                     EIIARQMFSRYLIKDPGDTSMLPGETVEAEVFEHANQVAKVKAKADRLLLGITKASLT
                     TDSFLSAASFQETPRVLIEAAVNGKIDYLEGLKENVIIGCLIPAGTGYKGKKVRDKYE
                     NIQPPAVSEN"
     gene            complement(32235..35570)
                     /gene="rpoB"
                     /locus_tag="PKK65_00595"
     CDS             complement(32235..35570)
                     /gene="rpoB"
                     /locus_tag="PKK65_00595"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNU81221.1"
                     /translation="MSNVSSTDKKIAERKFFTDFKEVMPLPDLIEIQKDSYRWFLEEG
                     LAELFDEINPVTDFIGRDLELYFEDYYLDEPKFSETESRAKNITYEAPLRVKTRLLNK
                     KTNQSSNQEVYLGDFPIMTDKGTFIINGIERVVVSQLIRSAGVMFTAEFIKGKKCYGA
                     KIIPNRGAWLEIETDSNKVIWVRIDRKRKVAVTSLLRAFGYENDEELKKLFRDVDLVK
                     SNDEPTFIEATIEKDAAKNEAEGLQEVYRRIRPGDLASVENARQLIHSMFFRFDRYDF
                     GRVGRYKLNRRFGLDLPNKKENRVLRKEDLLLIVKEVIRLNITKEPEDDIDHLGNRRI
                     RAIGGLIQNRFRVGLARMERIIKDRMSTSDIATLTPNKLVNSRPVIGVVKEFFMSSQL
                     SQFMDQTNPLAELEHKRRLSAMGPGGLTRERAGFDVRDVHSTHYGRICPIATPEGPNI
                     GLVGHLASYARLNSYGFLETPYRRVKHEKSGSRITDEVVYLDAFEEEKYITADATIPV
                     DASGHLAVSRYEVRKFGQPGVEDVNKIDFVGVAANQIISIATSLIPFMEHNDGQRSLM
                     GTNMQRQAVPLVKAEAPIVGTGVEARAARDSGHVAIAKEDGIVVKSEAALIEIENKKG
                     QISQYHLNKFLRSNSSTCINQHPLVTVGEKVKAGQILTDGPSIDNGELSLGRNVLVAF
                     MTWDGFNYEDAIIISERLVKEDIYSSIHIENYTIDVRDTKLGPELITNDIPNISEEKL
                     KNLDEDGIIHIGAEVSSGDILVGKITPKGETTLSAEEKLLRAIFGEKAKDVRDSSLYL
                     EHGEHGKVVDVKIFSREEGDRLAAGVLKSIQVSVANLRKIQVGDKMSGRHGNKGVISK
                     VVPVEDMPYMADGTPIDIILSPLGIISRMNLGQLLETHLGFAAKKLGYRIATPALNGV
                     SEEQIKRELKKAGLPEDGKITLYDGKTGEAFDHKITVGYKYILKLNHMVEDKIHQRSI
                     GPYSLITQQPLGGKAQFGGQRFGEMEVWALEGYGASHTLQEMLTIKSDDVPGRSKAYE
                     AIIKGEEIEKLNVPESFNVLIRELKGLGLSVELLGGSNGQTASEVETEIDLEKKKDPR
                     YDYDKPEIIATSDSVEAALPTEE"
     gene            complement(35961..37373)
                     /gene="murE"
                     /locus_tag="PKK65_00600"
     CDS             complement(35961..37373)
                     /gene="murE"
                     /locus_tag="PKK65_00600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramyl-tripeptide synthetase"
                     /protein_id="HNU81222.1"
                     /translation="MLARIRRLIPTTIFQSLQPFYHFIMGYLAALYYRFPSQQMLIIG
                     VTGTTGKTSSVYFLSRLLNANGYKAGYTSTAQFNDGEKEWLNDKKMTMPGRFFLQRTL
                     RRMVNNGCQAAIIETTSQGIEQFRHRFINYDLLVFTNLYPEHIDAHGSFENYKRAKGK
                     LFSHLFRCRSKYLDDNNQVVSHPSGLKQTELLKVKKTIIVNGDDDQASYFLSFPAERK
                     CVYTLNPNLVATSLTDDSGQEIQPPPEIFLYEPLFATASGSDFQFNGQRCHLSILGSY
                     HVFNAVAALTVASVLNIKTEQAAAALANIKSLAGKMEKIEAGQNFHVLIDYAFEPKAL
                     EALYQNLNFIHYRRLIHILGSAGGGRDQARRSILGKMAGEKADIVIVTNEDPYDDNPQ
                     DIINQVAAGAEKAGKILESNLYKILDRREAMRLAFRLASEGDLVLITGKGAEQYICGP
                     RGSRIPWDDRVVAREELAYY"
     gene            complement(37511..40090)
                     /locus_tag="PKK65_00605"
     CDS             complement(37511..40090)
                     /locus_tag="PKK65_00605"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNU81223.1"
                     /translation="MGKKKKKTANPFENLPNFELSDHAKHSIGIVIVFAFGLISLLGL
                     FNLSGRSGVFLSHWLSLIFGFGKWLVPLVLLFWGILLVRNKTVDVKPSDYLGLFLLFI
                     SYQTLFHFFLKEADWLSNAQAGLGGGYIGYYLSRLFLYAFGFWGGVIILFCLLLVALV
                     IVFNTSLSKLIGRESFLANFGRTLGNAFRGVFSASSAERAGKGEAEDTIESTQSEVLA
                     SWRKRELAESESDEDAEDKIDSELSAKQANLAQAATSKVQAKLSLPKKKIKIDLPIEL
                     LNHKSGQAVGGDIQRGAALIKRTLENFGIAVEMAETKVGPTVTQYTFKPAEGVKVSRI
                     ITLNNDLSLALAAHPIRIEAPIPGQSLVGVEVPNKVKAVVGLREILEDPIFKNRKHNL
                     MIALGKDVSGTVWIYDITRMPHLLVAGATNSGKSVCLNTIIISLLYQNNPDDLRFIMV
                     DPKRVELPVYNGIPHLLTPVITEVSKTINALKWCLNEMERRYDLFNKAGKKNIQSYNA
                     DKPEEKLPYIIFIIDELADFMMTSGKEMEAAIIRLAQMSRAVGIHLILATQRPSVDVI
                     TGLIKANIPARIAFSVTSLVDSKTILDMSGAEKLLGQGDMLLTTTELSKPKRIQGAYV
                     SDQEINDVINYIKEKSGAVDYLEGVTDRQKVGGVAGVGLDGTYGDEDELMSEAQEIII
                     KAGKASTSLLQRRLSIGYGRAAKILDMLEESGIIGPANGSKPREILISKEQLENMQNQ
                     GISAMPVHNRHEMSAPDHYFGNDEGDDDTLSFKSGDEDKEVVKETEIESKTEEDEDNN
                     QNVPAFLLDDNGPEDDVQEENEILEEIKPEEPSPPRQAEEKKQTLKSRVFDDDDEGMF
                     FSR"
     gene            complement(40333..41742)
                     /locus_tag="PKK65_00610"
     CDS             complement(40333..41742)
                     /locus_tag="PKK65_00610"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNU81224.1"
                     /translation="MLKIKKKILFCAAEAAPLAKIGGLADVVDSLPPALKKQGVEVRV
                     IMPAHGSISLRFWRAKKLASFSVNFGKKKERVVLLSAKVEGVHYYFLQSKNYFSGAVY
                     GENTLDKYLFFSQAVAASMPFLAFTPDIIHAHDFHTAGVLLTYLQKSDYPRPKLVLTI
                     HNLQHQGWVSASKFKSFGFNPDIVPAAAKNKAGNWVNILAAGIMAADKITTVSPSYAQ
                     EIMTKKYGQELEKLLISRQNDLVGILNGLDVVSYNPATDKALAFNYNRINLVAGKAIN
                     KQALRSYFGLSDSPIPLLALVARLSDQKGIDLFNLNKLKTLIKKYPFQLIILGTGEKK
                     FESLASSLAVNLPEAVRAEIAFDDVLARNLYAGSDYFLVPSRFEPCGLTQMIAMRYGS
                     VPIVRATGGLKDTVIDGYNGFVFKNYTDSSLLSSLQRALRLYYQEPDKYQALRRAGMS
                     ADWSWQASVPRYLDLYNSL"
     gene            complement(41729..42775)
                     /locus_tag="PKK65_00615"
     CDS             complement(41729..42775)
                     /locus_tag="PKK65_00615"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HNU81225.1"
                     /translation="MNKILSLFDKQTVRLLLEKEVLPLYPEFEKIVSFTIKPYKKLIW
                     TTTYHVVVAYRLVLAKANGEEHKIEIVCSAHSHEARENVFKVLNFLWNSGLPGDGIAL
                     PRPLFFNSEFNGTFYRAIEGKNLLNFIKLNNREKVKSMVVQAAGLFARLHALSLPANA
                     KEIFVEDNSLLRTVVPGRDLIVWEIGQRFQEKYVHDIADFYDRFIKQEEEFFASTTKR
                     WLIHGDAHPENVIAVGADKIGLIDFTDFCLADFARDFGAFMQQLEYKIKRNLQDDEFA
                     LAMKRLFLNSYLQAANISLDESLQRRLDLYYNWTATRTATFWLLKHECEPEKAEAMIA
                     LVKNYLNTNHHAQD"
     gene            complement(42828..43988)
                     /locus_tag="PKK65_00620"
     CDS             complement(42828..43988)
                     /locus_tag="PKK65_00620"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81226.1"
                     /translation="MLWLNFLHLYQPANSPSARIKEAVEKSYLRLTQLLEDNPDLRFT
                     ANISACLLERLRTDGFIELINRWRRLILSGRLEVVGSAAYHAFLPLIPEREALYQIKR
                     QEELLQEILGVDVRGGGFFFPEMAYTPALAKLVKAQGYRWIIIDEASLPHLLERNPNG
                     AYLDANSGLQVIVRQRRFSNAYAPDIIGDLLESEVAHELLITATDAELYGLRHEDPTL
                     ELEKIVALDNLQTQNISDFLDTQKSLVPVIFRAASWETNWDEDGRNPFSVWYDTNNKI
                     QRLLWRLARMAIAAGDKYGDDKNYQWYRWHLSRGLASCSFWWASGRDFFHNFGSVAWN
                     PDEVENGINDLLRAIRSLQDKRSLKEKIKAENLVSTIRARLWQRHWRQFWTA"
     gene            complement(44002..45468)
                     /locus_tag="PKK65_00625"
     CDS             complement(44002..45468)
                     /locus_tag="PKK65_00625"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HNU81227.1"
                     /translation="MRKILKIVHIASEVAPFSKTGGLGDVARSLPKSLSRLGHDVCLI
                     TPLYTKSINKTEYKLKLIFSNVKLRLNSDETIIVNYWQGQLLENLPVYFIECGKYFSK
                     HKALYGASNENARFLVFDVAALKLLSLLKFEADIIHCHDWHTGLIPYYLKHDFRYSKT
                     LKNAKTVFTIHNLVFQFGHNWWEVPAAEKDYGRGPIPHLDDPRLERINFVKRAILSAD
                     IINTVSEQYRSEIMTKKFGQDLHQILRNRSDRLFGIVNGIDYYAYNPEKDQTLSNNFS
                     WRNLSGKTENKFHLQKFFGLPIGRNIPVISMTSRITFQKGFELMLKILPDLLNLEVQL
                     IIIGTGDKKYISELRKFARKFPKKLVVIPSHKANQQYETQVYAGSDLLLLPSNHEPCG
                     INQLIAMRYGCVPIVREIGGLYDTVEDYNPATGRGNGFTFKREDPADLFRAIIRALEN
                     YRNRSAWTKLVQRVMKQSNSWEIPAKKYIQLYRRVKHL"
     gene            complement(45473..45955)
                     /gene="ruvC"
                     /locus_tag="PKK65_00630"
     CDS             complement(45473..45955)
                     /gene="ruvC"
                     /locus_tag="PKK65_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005956739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNU81228.1"
                     /translation="MEKIIFGIDPGIADTGYGFIRVQGGNLECLDYGSIKTPAGQPLP
                     ERLQSLYQELRLLLEKYRPQVAAVEQLFFNKNVRTALIVGQARGVALLAVSQQGIPLL
                     DFTPAQVKRAVSAYGQASKLQIQKMVKLILKLEEIPKPDDAADALAVAICGANQRQWR
                     "
     gene            complement(46027..46749)
                     /locus_tag="PKK65_00635"
     CDS             complement(46027..46749)
                     /locus_tag="PKK65_00635"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNU81229.1"
                     /translation="MSGHSKWATTHRQKELVDAKRGAIFTKLANVISIAAKAGGDPSA
                     NPSLRAAIDRARAASMPKDNIERAIKKGTGELAGEQIEEIYYEGIGPAGIQVIVKCVT
                     DNRNRSVSTIRHIFTKNGGSFGAVMWNFEQAGVILLSQAELAAKNLSVADLELDLIDN
                     GASDISVSKEGLTIYTELNDFQKMQDFLKSKKLKTELAEIEYVAKEKVNLSELDQEKC
                     DKFLEELDDNEDVSAYYTNLAD"
     gene            46856..47776
                     /locus_tag="PKK65_00640"
     CDS             46856..47776
                     /locus_tag="PKK65_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81230.1"
                     /translation="MNKLALDSLNLSVLKALAFFQKNPPPRFQLKKYPFPLVVGSGNA
                     INVGLAIFDQQKAIFANESNFRLILKRYRDLIKQKNISHAVIISASGEKDSVWEIKEA
                     KKAGLKTVLLTCSPASRAAKIAHETYIFPKLSEPYTYNVSTYLGMIMAATGEKASTIT
                     QFIFNLKLPKNFKKYRAYSFILPDEWGAIALMLEIKKSELFGPNLSLRAFSYGESRHA
                     KFVIREKDELVISFGKNKYFGHPQHRWEIKKPKTANKAWAMAVSYFLIGLIQDAHPDY
                     FRPNLVKFCRDYGYKAYAGAKPFDIIVPGN"
     gene            complement(47891..48628)
                     /gene="gpmA"
                     /locus_tag="PKK65_00645"
     CDS             complement(47891..48628)
                     /gene="gpmA"
                     /locus_tag="PKK65_00645"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HNU81231.1"
                     /translation="MLKLVLLRHGESVWNEKGLFTGWTDVDLSSRGLLEASSAGKKLK
                     KAGFSFDLAYVSFLKRAIKTLNIVLEEMDLLWLPTIKDWRLNERHYGNLQGLNKQQTA
                     KKFGEEQVKIWRRSYDVRPPEIDNKNKYNQKDDVRYQDIKVPSAESLKDVVLRVTPWW
                     KKEALPLLRKGKKILVTASGNSLRALVKYLEAMSENEIVEFNIPTGIPLVYELDKNLR
                     PVKRYYLASAKELKAAIDKVKNQGKKK"
     gene            48834..50156
                     /locus_tag="PKK65_00650"
     CDS             48834..50156
                     /locus_tag="PKK65_00650"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNU81232.1"
                     /translation="MKNLIAVTVTTVIKPFFWLGKFLLYRPFIRLYYLIFRIKKYDLK
                     QQSKRELINKKSLHLTALILIISAIILNLSDKKQTSAAVGKAPKSLMAEIVKNEFSDL
                     GQNNDELVEETAQNINMHALGAEKYLGGRDAVSVVKSVTPVSEEENNLAKNTNNMLTK
                     PKQIAIINNQTEKPAENNTTTSRQEITEYTVRAGDTISSIARRFNLNVNTILWANNLN
                     AFSIIRAGDKLTILPTDGFLYKVVRGDTIGKIAQTYDVSADKIIASNSLDAAGTITIG
                     QQLIIPGVKKPTSHTVASAGTQSSYSGIAVIKDIISSDKPIVSSNKMVWPTVGNRITQ
                     YFSWKHSGLDIANKVGTPIYAADDGVVEISQGGYNGGYGNTILINHGGGKKTRYGHVS
                     KLLVKKGETVTKGQLIALMGSTGRSTGPHLHFEVIINGVRYNPLNYIK"
     gene            50158..50400
                     /locus_tag="PKK65_00655"
     CDS             50158..50400
                     /locus_tag="PKK65_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81233.1"
                     /translation="MIILGLIIIAVGTVLIIKTEWFLQNFGRLSWFENKLGSEGGSRL
                     GYKLLGLVAIFIGIVLMSGNGNDFFTWLLGPLIPKQ"
     gene            50457..50529
                     /locus_tag="PKK65_00660"
     tRNA            50457..50529
                     /locus_tag="PKK65_00660"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50490..50492,aa:Ala,seq:tgc)
BASE COUNT        15820 a        11577 c         9494 g        13590 t
ORIGIN      
        1 ctgtagggta attataatca taaacacgaa caggcggatc ggcaacagtt gcttcgttca
       61 tattagaaca gccttcaccc tcgtcacaac catgttcatg gccaacggta taagaaaaag
      121 aagaaaaacc gtcatattcc ccccaaccaa tataataagt ctccccttca caccctccca
      181 tttgattatc gagtgtaatt tcctcggttt ttattagtaa cttatcttgt ttggggacgc
      241 atattttttt aatataacct cctttttctt cagatatcca catttcataa ttatcatcat
      301 ctaatgggtt attccaagtc acacaaggcc tcggcttctt attgctggtt gatttataac
      361 acttcgattc ttctacggac tcccccccct tcaaatcttc aatcttaaaa ccaacgcgtt
      421 tctctaaaag aacaaaattc ccattcgctt gcgtacagta attcaaagga taggcgcctt
      481 gataaccaag agtggagcgg gctgatttag cattagcgct aatttcatca atcggatacc
      541 aaagtaaaca gacattctta ttggctaaat catgctgtaa acaataaccg accaaaccgt
      601 cgctcaccac ttggctattc ttgctcgtac aagaagtagc gtcttgagtc ggatataaac
      661 ggcactcctt agctacatat ttatccttac caacctgcaa aaccggctcg atattgatat
      721 cgtcaaaata gacatagcgc tcctctttaa cattcgaatc cgagccgaca taaatcttaa
      781 tggacgtagt cgtgccgaca tcatttttcg aaattttaaa tacctgacgc tcccagccgt
      841 tcggagaatt agcaacggaa ctggcgatca atgtattatc aatggcatca tatatccaga
      901 cccggccatt caagcccgaa cccttagtat tgagcagata attcacataa taatcttgat
      961 taggctgaat ggaaatcggc gcattctcgg catgaggaga aatctgttgc gaagacatga
     1021 ctttcagata agctctgcct tcacccggat aatcagtggg ggcattttta ggattattga
     1081 tgatactatc ggcgacaatg ccgttatcaa aagaacagtc gttatgagga gggttaacat
     1141 aacggcaagt tagtgagggg ctgccctctt caaaattata atgcgcctcc gtattcaagc
     1201 ccacttcttt catatccgca aaataatact gatttaagac ggaataaccg tcaattttag
     1261 acattacagc gatatcagat tcgccattaa gtatagttcc tttaccggaa ttaacgaaat
     1321 tagcacattc gttattatca tttaaattat tacattcacc cagagcataa caggtgcgct
     1381 gattggtaac gggatcaact acataagtga gacaatcgag ccaagtggcg caaacacgat
     1441 caggcctaac ctttattaaa gtattagcgc tgcaattacc gctcgtgcaa ttaaccggcg
     1501 cttggccgtt agtgctggcg aaggcgtccc agcccccaga caaagataac agaccctcgc
     1561 cgccgttaac cgaacgttca ttaaaaagca cacaaccatc atcaaaatta actaaaccat
     1621 tacagctttt tttatcaata ttctgcttaa attgataatc gactatagcc tctttaacgg
     1681 aaacaaaata accactgcta gcgccactta ccgtcgcggc cgtattgccg cgagaataga
     1741 aaataaatct cttactcggc tgattggcgg gaatggtgag agtgctgctg gcacttgtac
     1801 tcaaagtatt atcctcttga agcagagaca ctccggccgg gaaagtcaaa gaaacagaat
     1861 agtcgatagg attgctgcta taactatcaa gagcaaaaat atacagctta ttaggttcta
     1921 ggacgatgtc ttgagataaa ggataggagc aagaattatc agtactagaa gactgccaac
     1981 cgttacaatt attgtctaaa gcggcgttaa aaattaaatt aggagaaaag ctgctaacgg
     2041 ggtcaatgta ttcggtgcaa ccagaggcgg caatttcaca caaaccggcc gcttctgaag
     2101 gcaccggtac ttgattgccg ccaccgccga aaccgatcgt ccggttatat gagaccggac
     2161 attcatagtc gttattatca accaaacagg cacggtcggc actgcagtca tcggcactct
     2221 gacaaagagg agaggcctgg ctttccgtag cgtcaatctt cccgttatta ttcaaatcgc
     2281 aacgcattac cggcacttta taccaagcct tgcccggctt atccggatta atactgctgc
     2341 gatacacaca agcgttattg ccgggattct tgaaatagga aacaccactg ccatcaatat
     2401 actgccacgc ttcgcaacca gcctcactgg cgttacacaa agaattatta taattatcag
     2461 gctgattcaa tttaaagaca tcactgtaat cagtggtagt cccggtagaa acggcttcac
     2521 ccaaacgcga acagccggaa tcggcacgat tacacagata ggaagaatca taaattgcgt
     2581 agaagaaacg atcgccggca acactgacgc attccggttc gttggcatca cattgaccgt
     2641 taccgtttcc gtctttccaa atacccggct tatagctatt gtagttggcg gtatttacca
     2701 tcagctcgca accgacggct gaatcttgac ataaacggct aaactggcgc aaataatgac
     2761 tattaccggt ccggtcgtta tacagagaac aaccaagatt ataattagcg ccccgataat
     2821 tatccaacat gtcataataa cagacatcgg gcacctgact gctgttctta atcaaataat
     2881 agcggtcgct aatggcggta aggataaggt tatcgagata gaaatcatga ttagcaacaa
     2941 tgattaaaga ctcgttagca tcaactacat gattaagttc cggcaaattc aattcgaaag
     3001 agcgccattc actatctccg gaaatagaga tgcttccggt tggattgcta tcgctggcgt
     3061 tgaaatatgt aatttctccg ttactattac ggaaaccaaa gctaagcgtc gtttccaccg
     3121 aggcggccgc cgtaaattta agactatacg attgaccttg gaccgcttgt ttgccgataa
     3181 ttttccgaac atgagaaggc agagcggcat tagccttacc aaagaaacgg tcaaaccaac
     3241 ttaattgagt tgggttgtca caactagtcg actgaatgct gcccgtggca ttagcaccac
     3301 tatcataaaa taatgactta ccgttattag tactaacgac ctgcgagctg agcacggtat
     3361 caccgcactg tccctcccag ccctccaagt tattttcaaa gctatcagcg tcaactaatt
     3421 tagtattatt gcccaagcta ccattatact cacggcaacc attttgactg gcagcgcatt
     3481 taacgccttc attggggatg gcctgataaa tacaagcttg atgctgcgta ctccaagttc
     3541 ctccgttctt acaaacgtaa cagaccgaag aagcgatgtt ccaaaaacga tcgctgccgc
     3601 tacactgcgc ctgcgtcaaa gtagtatcaa tattattctc agagagccga taaggatggc
     3661 aattttcgga acaggtaata gtataagcag aaagatgata ggaaatagcg ccggctttat
     3721 tgtagaactg acggcagtca gggttaaagc gaggatcgct cggcggtaaa ttgaaaatag
     3781 tttcattaca ttccgccgaa tcgtcagcgg taactgccgg agaaccgttg gcatctttct
     3841 tcaaaactaa agcctttaat tgatagccgc tttcttccgt gcccacccaa gtatagaaat
     3901 cgccgcagct ggcaccgggc ttaatgcatt gctttaattg agtataatat tctttgccct
     3961 cgccgccgct ggccgccgcg tcaagattag taaattcgct acaaccgact gcctccgcgc
     4021 tgcaagcgcg actattttta ggaataatct tttctgcgta aggcgaatag aaataatttg
     4081 cccgcgcaac atataaatca tagccgtcac aactggcatc acaataatca gaagaccggg
     4141 cttgcgccgc caccgagaaa gcagtgctct tttcggtata acgttcgcaa ccaacttcgt
     4201 cacgattaca ctgacgggca aaattcttac atatttccgg agcgtccgcc tttaagcgat
     4261 aatcgggatt acccacatta gcgttttcat aacaaacagg ggccaaatac ggagggatta
     4321 atttctcata aacattatag gagccatagg cggaaaagcc agagtcccaa ggagacattt
     4381 ctaattttag atttctcaat aggaaacgag tctcactggt gctgctataa gcaaccacag
     4441 aaaaagacat ctctgacaga ggcttttctg caagattacg cgtgacagac aaatgacgcc
     4501 aaacatcgtg gtcggtagtt gaaaccgtgg tcgcataatc tccacctaaa actaaatcaa
     4561 tcttatcacc ggcaagaaga tagacatcgg cgcttaaagt ataggtttca tcaaaaagaa
     4621 tatttaaatt ggaggggata agcgacaccg cgttatcaga aaagatgcca ataggagtac
     4681 cggtggaatt acccaccgat tctaccagta tcgccttacc ggaagagttg ctgtcaatgg
     4741 tggaaacatc aataatttcg gcagctttat tccccgttga ccaaaccggc caataattat
     4801 taattttgcc attaactagt tcatcgccga tattgtcttc ggcaaaatca gctcccatta
     4861 ttaaattaga gccccagccg ggtttaatgc gcattacttc ggaacagcct tcctggctgg
     4921 aagagcaaga aaataattta ttattaaaat aagcggtcgc caaaggattg tcagaccaaa
     4981 ctatctgccc attttcatta ttataactac cattaaaaga atatttttta cagccggcgc
     5041 tatcagcgct gcaatcgcca taatctaaag tattttctaa ataagcaact ctctggccac
     5101 tactagaatt taagaaagcg ctacaagtat tatcaatcgg cttacaggat ttggaagcaa
     5161 aactccaaat gcggcgctcc gattggcagt agccgtaagc ttcgcaactg ccgtccgccc
     5221 tttctttaat acaagtcttg tcatcggcgc aataatcatc agctctggta atttgaacat
     5281 cgtcagcgat ggtgccatca ctggcagcgc tcggcacaac ggctatatct aaaatctgcg
     5341 cgctataacc ttgtttagca caataattaa gaggcgcttt caaaacccaa ttaggatcga
     5401 ctaaaccgcg acaccagcct tgattgcgca catcaaaatt agaggtgtat tcattgtagg
     5461 catcagtagc gctaaaacat gacattaagt caattaaagt agcttgcttg ccgaaagcgg
     5521 cagcggcctc ccaaccgata gggataatcc gatacttgcg cattatctgg agattacgca
     5581 agttataggc gccttcgcga taatcactag taaatagcca ctctttatgt aaataaccgt
     5641 ctttgaccgc ttccgctacc gtcttttttt ctgtaatccc ctgcattaat ttatcatcaa
     5701 taacacattc ggtcggaccg ggattattgc gatcgcgaca catcgttaaa gaagacaaaa
     5761 tgtcatagtc agcccggacc gtaaaatcag gttcgataat actggcggcg ctttcacgaa
     5821 cagacacttc cccatagact acattggggt cagtggcagg attactaatg gccgcactat
     5881 tgttatcgga ggaaatcttt cctaaattac tcatgaattt attgaaagcg gtgagagcta
     5941 gttgatttaa gaaaacatta gcggcatcaa ttaaagcatc gccggaaaaa gtcgccatat
     6001 tactaatata attctgttca gacatctctt tttttgtttc tgcctctccc ggcagaccaa
     6061 ctaaattgcc ggcaatatct cgcatatcca gccaaccgcc gtccgcaatt aatttagtct
     6121 tgctgtcttc cttcttctct tctatcttct tggtcatatc cgactgcaaa gtcaagtaaa
     6181 tgcctaaatc attggaaact ggattaaaga ttcccttaaa tttatctaag aaatcatcct
     6241 ccttaaaagc aatcatgcgg tcaatatagt tatcccaatt ctgaacaagc gccgaagcgg
     6301 tacaattagg agcggccggg cgattataat cgaccaatcc catggcaata gtcagcttag
     6361 ccgaaatcga aggttggcag acagcgacag ttttggctgt tctcgtggtg gtgacaccgt
     6421 cagcgccagt gtaaacattg ctagcattat tttttcctct acaggcatta aatgtattaa
     6481 ggcaatcatt aagacagtca ttaaattcat cgtttgataa ttcatcgata gaaaaactgc
     6541 aattgttctc gcactcgtta agtgcttctt cacattgtgc ttcgttttgc tctttcaact
     6601 tgctgttatt ccaattatta accgtctcct cgataaattg accggcggcc tcatcgccaa
     6661 tttgagccaa gtaactgccc cagccttggg tgacgaacat cggcttctgt ccttcctttc
     6721 ctgaacccaa ccaagtggcg gtatcataag caattttatt taaagcggtg cgtacggttt
     6781 tctggaaagc agccgcgccg cctttttcat aggctttttt caaaaaagcc caaattggcg
     6841 tcaagatgtt attaactgct tgattaacat ctgtgcccgg aaccgcctgg gcttgaaccg
     6901 gttttagcgg catcagcaaa aaagagattg aaaacaacaa caaaataacg gccactccaa
     6961 atttatgccg cttaataaaa agatttgagt gcttcttcat agttttccag ataaactata
     7021 tcaaaaaata ataaaaatta ttggctaacg ccgctttggc cagctaacat ctcttgataa
     7081 gtctcaatca aatcctcgtc gcttaactgt tgaagcatgc cggcatcagc gccgctgtct
     7141 aataaaaggg cgcgcaactg agcggcgtta gcttgaccgg acatcatcgc ctgcaaacta
     7201 tcgctattac tactatcagc cgtaacagta gccgtggccg tagagatggt agcggtatta
     7261 ctggcagtcg ttgttaagac ggggatatct tgaacattac attgctgccc tggcgcgcaa
     7321 ttaggattag taccgttctc aatttctgcc cgatcactaa taccatcgct gtccgagtcc
     7381 tccaaaaaag gtgaggtgcc ataaagattt gtttcctcgt tgtcagtcaa gccatcgccg
     7441 tcagaatcca tattagagag ggcaagcata aaatcgtcag tatttagtga gttttctgta
     7501 ttatttgagc cgggaatgga aaacgggctg gttaaacgag aatgaaactg ccaaacccat
     7561 aaaacaataa cacccaaact cagagccgag aggaacagaa aggccgttcg ctgtggtttg
     7621 ggcaaacttt taaaaaatga gggagagggc tcattttttt ttgattcttc aaaattgtcg
     7681 cgcatagtaa tctttctgtt taaaaaataa aacaaaaacc agattactca aattataaca
     7741 caaaatcagg tatagccgca actaaaagac gccactgttc tacgcttaaa tcttcagctc
     7801 gcgctttaac ctgaaggccg actttaatta aagcgccagt aatcaccccc ggctctaact
     7861 ttaaaccgcc agataaatta tttttcaaca tttttcgctt ggcactaaag ccgatacgcg
     7921 ctaaacggaa aaatttttta tcaaattcta aagattcttt agttgtcttg tttcgtaaat
     7981 aatcaaagcg aagaagggcg ctatcaaccg ccggctgtgg ccaaaaatca gaagccggca
     8041 ctgttctgat aatttgcgga tggccgtaat actgaaccga caatgataat agagacatct
     8101 tcggtggttg agcgcaaatt ctttccgcca cttctttctg caacatcaac accaaagaac
     8161 gaggcggacg cggtgaagaa agaaaagtac gcaaaaaaaa ggaagtgata ttgtagggca
     8221 aattagcaac taccctataa ttttcaccag aatcaaaata tttatccggg ttaaagcgca
     8281 aaatatcctg atggacaatt tcgacattgt ccacaccctg agaatcaaga gcaatctgca
     8341 aaagagcagc caatttatca tctaattcaa ccgccaccac ccgcttcgct tttttagata
     8401 aagccgccgt cagaaagccc aaaccaggac caacttctaa aatggtatct ttcttgttaa
     8461 tatcggcggc cgcaatcatc gcctgataaa taccgtcatt aattaaaaaa ttctgtccct
     8521 tactacgcgc gggcttaatt tcataaagac ggcataattc tttagttctc gcagccaaac
     8581 tgtccatatg gctaaaccac aaaattaatc agccgaccgg gcacaaaaat tcttttcttg
     8641 atatcttttc cttccaacca tttactgacc gccccctcag agatggctaa gtgcacgatt
     8701 tcctcttcat cagccccggc taatacagtc agccgtgccc tgagcctacc gttaacctgg
     8761 acaatgtaag tgacggtctc atctttgatt aactcctctt gataagcggg ccattttgat
     8821 ttaaaaatac tcccggtaaa accaagctta tcccataatt cctcactaag atgaggggca
     8881 aaaggcgcta aaatttgcaa taattttcct agattttcag acgataaagg catggcttga
     8941 tgagcctggc aataatcagt cacgctatta attaaaatca taaaggcgga aacggccgtg
     9001 ttgaaacgga aagcctcaat atcttcatca actttcttta ttgttttatg gagtaaagat
     9061 aaaatttgtt tttcttcttt taccggcaac ttattactgg aatcaacaat attataaact
     9121 ttctctaaaa atttacgtgt tcccgacaaa cccttagtgc tccaagcgac cgtctgatca
     9181 aaggggccca taaacatttc atacaaacga agagcgtcgg cgccaaagcg cttagccatc
     9241 tcctcaggat taataacatt gccccagcgc ttgctcattt ttcggccgtc ttcacccata
     9301 attaagccaa catgctgcaa ttttataaaa ggctcctcat aattcacgac gccaatatca
     9361 taaagaaatt tatgccaaaa acgagcgtaa attaaatgtc tggtcgcatg ttcggcgcca
     9421 ccaacatata aatcaactgg cgaccagtac ttttctaaat ctttagccac caattcctgc
     9481 tgattatgag gatcaatata ccgcaggtaa taccaacagg aaccgcccca ttgcggcatg
     9541 gtattggttt cccgtttggc cgggccgccg cagtgcggac aaacgacatt aacccagtca
     9601 gatatcgccg ccaatggtga ttcgccggtg ccggtgggcg cataactttc tacttctggc
     9661 aaagttaccg gcagttgttc ttctggtacc ggcacgacgc cgcatttatc gcaatgaata
     9721 agaggaatcg gttcccccca atagcgctgg cgcgaatata cccaatcttt aattttgtaa
     9781 tttacttttt cccggccact attattcttt tgtaaccaat caatcatttt tattcttgcc
     9841 tcagaggatg acaaaccaga aaaatcacca gaatcacgta aagtgccgtc tgtaatcaaa
     9901 gcctgttcta aatttaacga ctctctattg tttgtaataa taacctgcct aataggcaat
     9961 tgatattttt gagcaaattc ccaatccctc tcgtcatgcg ctggcacagc catcacggca
    10021 ccggtgccat aggaatttaa gacataatcg gcaagataaa ctaagacttt ttcttgatta
    10081 aagggattga cggcaaagac gccttccaaa cacacgccgg ttttttcttt attaagatcg
    10141 gttctttgta attctgtctt atccttaact tcttgcaaat aattttcaac cgccaaccaa
    10201 ttgtttattt ttggctttaa actcatgacc aaaggatttt ccggcgctaa aacgacatag
    10261 gtacagccga aaatggtgtc aattctggtg gtaaaaaccg taattttttc ctcggaacca
    10321 aaaatttgaa aatcaatctc ggcgccaagg ctgcggccaa gccaatgacg ttgcatttct
    10381 ttaatcgcct cctcccaatc gggcaactta tctaaatcat caagcagacg gtcggcatat
    10441 ttagtaattt ttaaaaccca ttggcgcatc ggtttctgct cgactaaact gccgcaacgc
    10501 tcgcaatggc cgtcctccaa atcttcattg gctaaaccag tctggcaaga aggacaccaa
    10561 ttgataggag cgtaagattc ataggccagg ccacgccgcc acatctgcaa aaaaatccat
    10621 tgggtaaagc ggtaatattt ggggtcggtg gtattaattt ctctttccca gtcataagaa
    10681 aaaccaagca attccagttg tttcttaaag gtggcaatat ttttttgggt ggcttggcgc
    10741 gggtgaactt tattcttcaa agcataattt tccgccggca agccaaaggc gtcccagccc
    10801 atcgggtgga ggacattata cccctgcatt aaacgtttgc gcgccacgat atcggtggca
    10861 atataacctt tagggtggcc gacatgaaga ccagcgccgg aaggatacgg aaacatatcc
    10921 aaaacataat atttcggctt ttgcctatct tcgccggttc tatataattt agatgcgcgc
    10981 caacgctgtt gccatttttt ttcaattgct tgatgattat acatatatct taagctttta
    11041 tatttctata cctacgggag cgtgatcaga gccgagaacc tgatcgagaa taaaggcttt
    11101 ttttatcttt tttctaagtt tagcggaaat tacaaaatag tcaatccgcc aaccaacatt
    11161 ctttcggcgc gctcctgccc gaaaactcca ccaggaatat tgcacctttt tcggctccag
    11221 taaacgaaaa gaatctaaat atccagcaga caataattgc ttaaaccaat ttctctcttc
    11281 agtggtaaaa ccggcatggc cggcattttc tttgggtctc gctaaatcta tttcttggtg
    11341 ggcaacattg aaatcgccag caataataac gggtttatta ctttctaatt tattgatata
    11401 agccaataaa tcacgattaa aatcaagctt ataggctagg cgcgtcaatt cggcattagc
    11461 gttgggaaaa taaacattta atagataaaa atcagacaac tcaattgtct gcactctgcc
    11521 ctcatcgtca tctttagacg gaccgaaacc attactaacc ttgactttac ttccctgttt
    11581 gactaaaact gccgtgccac tataaccagg gcgcttggct ccatgccaaa aagaattata
    11641 gccttcaaat tctagccgaa ttttttccct ggcctcatcg ctaatttttg tctcttgcaa
    11701 acaaataata tcaggctttt cttgttgtaa ccaatcaagc aaaccctttt ttaaagccgc
    11761 ccttatccca ttaatattcc aggaaattat cttcataata ataactaaag caacatcaag
    11821 gggcaaatag ccacgaacgc ccgcttgtga tataataaaa ttaaaataaa tactaatata
    11881 ataaatacta attaagtata aagaaaaaat aagctttttt caattaaaca tatgagcatc
    11941 gatgaacgta ttaaatattt agaaatgaaa gaaaaacata aaaaaaagct gcgaccttgg
    12001 tataagaaac cttggggaat aataatcatc attctattac taggattaat cgcggccgtc
    12061 agcgcctccg ccttttatgt agccaataag gttcaagaaa ttaggaaggg gcagagcgca
    12121 gaacaacagg ccgctgaaat ccaaaaaatc accgccgcga ttgatggtcg aggagaaaat
    12181 tatagtctcg gaccaattaa cgctaaggta aaaatcgtaa ttttttctga ttatgcttgc
    12241 ccctattgcc gccaagccgc cacggttgtc cgtaatttag ccaagaaata tggaaaccaa
    12301 atacgtatta ctttccgcga ttaccctctg catgacaatt cggtagattt ggctttagcc
    12361 gttaattgcg ccggcagcca aaataaattc tgggaaatga acgagcttct gtttaataat
    12421 caagataccc ttagctctct gcaaggtccg gaactgaatc ttaaactcaa agaactggct
    12481 aatagtctgg gtttaaacgc ggataatttt gcctcttgcc tggataataa gacttatctc
    12541 taccggttaa acgatgattt ttccgatgcc gaactattag gtctctccgg cactcctact
    12601 tggtttatca atcgctataa aattaccggc ttttacccgg aagaaaattt cattggcatt
    12661 atcgacggtc tcttagccca ataatttaat tatgatacag ataagaatac atgggcgcgg
    12721 cggccagggc gtagtcaccg ccgccgaatt attagccagc gccgcttttc ttgacggctt
    12781 atatgcccaa gcttttcctt tatttggcgt ggaaagaagc ggtgctcctg ttcaatcctt
    12841 cgttcgttta gataaaaaac caattatcac ccgagaacaa atatataggc ctgatattct
    12901 aattatccag gatgacagcc tgctggcggt aaaagaaatc ttggtcggaa taaagcccaa
    12961 tagcatctta ataatcaaca gccgcacgaa aaaaacagac ttagcctcaa aactaaaaaa
    13021 taaaatcaaa cgccataata tttatccaag cccggcgact gaaatcgccc tggaactctt
    13081 aggaaaaaat ttggttaata ccgtcattct cggttctttt gctcaagtca ccaagcttat
    13141 ttcccttaaa tcgctgctgg cggcgattac cgataaattt gcggacaaag gcaaggaaat
    13201 aatcaataaa aataaaaacg cggtgttaaa atcctaccaa tatgaagaaa aagaataatc
    13261 cgcatcggcc ttttgcccgg cccggatcct ctttagacaa caaaaccggt gcttggcgaa
    13321 catatcgtcc ggaaatagat aagaatatct gcatcggctg ttcccgctgc gccaaactgt
    13381 gcccagacgc ctgtattttt atgaaaatgg ataaaaacaa taagttaaaa gccgaggtag
    13441 actacgatta ttgcaagggt tgcggtattt gtgcctccga atgcccagtt aaggcaatta
    13501 aaatgaccaa agactattaa tttagaattt taaatttaaa aacaataaaa attcaatctt
    13561 tgattcatgg ttttaaataa taaaacaaaa tttcttgagg gttcacgggc gatttctctg
    13621 actgtgtcta atatttctcc agaagtagtc tccgcctatc cgatcacgcc gcaaactcat
    13681 atagtagaag acctggccca actaaaagct gagggtaagg ctgattttga atacctgcgg
    13741 gccgaaagtg aatttgccgc cgcctcaatt atagttggcg ccgcggctgc cggcagtcgc
    13801 gtctatagtg ctaccagctc ccaaggttta attttgatga tggaggtatt gtataatgcc
    13861 gccggtctgc gtttgccatt agtcatgacc gttgccaacc gcgccgtcgg cgcgcccata
    13921 aatatttgga atgatcacag cgatgcgatg gcaatgcgcg atgccggctg gatgatgttg
    13981 tttgcagaaa atcatcaaga agccgttgat ctacatgtta tcgcctacaa actggcggaa
    14041 tcttataatt taccagtagc catcaatgtt gacggtttca ttttaaccca ctcctacgaa
    14101 aacgtcatta ttccagacaa aaaaactatc aagaaatacc tacctactta taagccggcg
    14161 ccgggtactt atcttaaccc gcaaaaaccg attactctag gtggcttctt tacgccggaa
    14221 cattattggc gggaaaagaa aaaacttgat gaagacttaa aaaaaactgg agcgagcttg
    14281 agccgcgcct accgcgagtg gaaaaaaata ctgccctcac cactcagcgt taaaccgata
    14341 tttgataacg gcttgattga atactgtgga ccgaaaaatc caaaaactat tttgattacc
    14401 atgggctctg tcgccggcac gattaaagaa gtcttaaaaa aagaaaaaaa tagcggcctc
    14461 ttaaaaatta gaagttatcg acctttcccc gctcaagata ttaagagaat aataaaaaat
    14521 tgtccgcgcc ttgctgtttt agaaaaagca agttccgctg gcgcttatgg acctctttac
    14581 agtgatgtaa tgagttctct tccccaatat cagggtttaa gcagcgatca cattattggt
    14641 ttaggcggac gtgatatcag caccgccatc atcaaaaata tcattaaatc agccggcaag
    14701 gccggtctta aattttggta atatgaaaaa tgcgaaagtc tttaatttaa taaaattagg
    14761 cttaataata tttacctttt tcggcttctt tctctttttt ccnnnnnnnn nnnnnnnnnn
    14821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14881 nnnnnnnnnn nnnnnnnnnn nncggacatc aaagatcctt taatctttga gccgcaaata
    14941 ggcttgccgg gcttggtcga taaaggtcaa caaattactc tcagcaacaa agatactagc
    15001 tacatcgccc aaatggtcaa aggcttttat aattatggca tcgccatcgc cggcatcctt
    15061 gccgccattg ttcttatggc tggcggcatt atctggctca cttccgccgg cagtagcgag
    15121 aaaatcagcc aggcgaaagg cttaattacg ggcagcctta ccggactcct cctagtattt
    15181 ggatcgtggt taatattaaa aactgtcaat ccttatcttg tagattttaa aatttctaaa
    15241 ataaaagcga ttcaaccaaa aactttctgc tgccatccga caagaggcaa tgtcatcacg
    15301 ataacagaca aaaacggtaa tgaatcttgt ccggatgcgg aatcaaaaaa atgtgaaaat
    15361 aatacgagat gtgaaaacgt tggtactaga gaggacaata aatttggctg tattaataaa
    15421 gaaaattttt attgctgcca atatgaaagt tcgaaaggtt acacatattg caaacccgtt
    15481 tccgtacatg aagtcataca aggagccaac tgtggcacaa tagcgccaac agattacaca
    15541 tgggaaaaaa catataacta ttattgccaa gaaaggagaa ttggaagcac caacataaat
    15601 tgcactaatc cctgcaccgg taagaataat ggggattatt gttatggaaa agacatagaa
    15661 gattcctgcc gttgttataa tgaaatggaa tggcacggac aaacagctta caaagaggga
    15721 gagccgtgcg ggatgaaaga tggctctatt tgcacctcaa aaggaactgg agggcaaatt
    15781 tttccgcgtt gcacagaaaa aggatggtct catgataaca atgcctggag aagaagggac
    15841 tgtggagaag gtttatattg ttgccatcct gattattaat atggacaaga aaaaaaataa
    15901 aattaccccc ggacataacg cctgcgctgg ctgtggccaa ttaagcgctg tccaagcggt
    15961 tatgcgcgct ctaagcgacg atacgatttt agtgaacgct accggctgcc tggaggtgac
    16021 gactaccgcc catccccaaa gctcttggcg cctgccctgg atgcattccc tatttgagaa
    16081 ttccgccgcg gttgccagcg gaattaaggc gactttaaaa cataaaaaga aaaaaactaa
    16141 agtagttatc caagctggcg acggcggcac ttatgatatc ggcattggcc taatcagcgg
    16201 catgtgggaa cgaaatgaag aaatcgtcta tatttgttat gataccgaat cttattccaa
    16261 caccggtatc caagcttccg gctctacccc ctggggagct aataccgtca ctacgccagc
    16321 gggaaaaaac agtttaggct ccgaacaaaa taaaaaagaa atgttggcta tcgccttggc
    16381 gcacggtctt aaatatgttg cccagagcac cagcgctttt ccagatgaca ttactaacaa
    16441 agttaaacac gctttagaga ctaatggtcc gtcttatatc caaatcttga caccctgcgt
    16501 gccgggatgg aaaatcaaaa ccgatgaagc tatcagggcc gcccgattag cggttcaaag
    16561 cggcctctat ccgcttttag agtataaaga tggaaaaatt atagactcta gcattaatga
    16621 taattttaaa gcctcgccgg tagatgaata tttaagaata caggggcgat tccgtcatct
    16681 gcaacccgag gaaataataa aaattcaaaa tttagccaat caaaatattg cccgttatca
    16741 tttatgatta aaaaaacaat ttttatctat cttttcctcc tgctttttct gccaaacata
    16801 gcggttgctt cggaaacaac tagggaaaat tgtagtctag aaacaattcc caaaggccaa
    16861 acttcggctg gtacctgtct tctaaaaagc aaacaagaag ccttttttcc taattgcgga
    16921 gacactctgc ccacaggcaa agtctgttgc tgtaatccta aaactagcga ctacacctgt
    16981 cgctggaaaa ccattatgta tgttacagac acaagcagcc ttgctcctgg tggtggatgg
    17041 tatggcgatt gcgatgccga tgaaaccaaa ggaattgact gtgatccgtc tacaatgcca
    17101 gaaacaaact acaaaacacg cgttatctgt tgctgcccag aggttacccc agtctacaaa
    17161 aaagctgact ttataatacc cgaatttcaa attcccatag acaccagtac cctgacagaa
    17221 gtctattgta ctggcccaga taattctgga caatgcgaaa taccgtggat agctgaatac
    17281 atccaaataa tatatcaata tggtctgggg gttggcggca tcctcgctgc catcgtctta
    17341 atggccggag gcgtactctg gcttgtttcg gctggcgatg tttctaaaat atcacaagcc
    17401 aaagacttaa tcatcggtag tattaccggc ttaattattc tttttggcac ttatctcata
    17461 ctgtctcaga taaaccccga gctaacaact ttgaaatcga tttccgtaaa aatgattgac
    17521 cgcatcatca tccagccaat taaaaatgga agtgacagta ctgagaatat ttccgataac
    17581 tgtgcggatg taaacagttt ggtgacgatt acggacattg ttgaaagtaa tgccgaaaca
    17641 ccaatgctcg ataaaatcgg caaggaaggt ctaattaaag cggttgaaga agccaaaaaa
    17701 caaggcgtag aattatatgt aaccagcgcc tttcgtaccg ccgctcatca acagagatta
    17761 tgggacgacg ccatggctaa atataaagac gcaaatttag ccgctaaata tgtagctaga
    17821 cctggaagct gcggcggcca ccgtagcggt cgggcaattg atgtctgtat taaagacacc
    17881 gcgtcttgtc aaaaaatggg aagtgcggcc gtggctaatt acagcgacga ggatgtcgtc
    17941 aaattaaagg cgattatgaa agcagctggc tggcaaagat atcgcggaga atggtggcac
    18001 tttcaatatc aagaaccaat gaaaaatgca gataactcat aaatttaacc aagtcaaaaa
    18061 ccgccccctt ctgagggcgg tttttaaaaa ataaaaatca attgaaagat tgattatccc
    18121 taaaatcatt tgcctctatc catcgcttca ttggctaatt tatctgcctc tttattcatc
    18181 tcccggggca cgtgttgaaa agaaattttt ttaaaactgg taattaaatt atgaaccttt
    18241 aaaaacaagg gtgccaaatc cttgtttttc actttatact catgattaag ctgcttaacc
    18301 actaattcgc tgtccaggcg acaaacaact tcttccgccc ccaaactttt agccttttct
    18361 aaacccgcca ggagcgcctg atattcggcc tgattattag tggcgcgacc gatgaaatta
    18421 gaaatttctg ccactgatac gccctgctca ttggtgagta aaacgccgat gccggctggc
    18481 ccgggattgc cgcgagcgcc gccgtcggaa tggatgatta ttttcataaa aattataaac
    18541 ttaaaattta attcttaatt ttttaatccg tactcttgcg ccaatcaata atctttaact
    18601 gaagctcgcg tcgcccgtta aattcattaa tttctaaata atacgctaaa tcaataatgt
    18661 cgccaatctg taaatcttta aaagtgtcaa aagcgttaaa agcaatcgcc ccaactgaaa
    18721 atatatcttg gctattagcc gcagctaagc gcaacttaat atgctgtcta tctttaccca
    18781 tccaaacaat gtcatcaacg cgcaaattag cactggtaaa cttaggctga ggattgttct
    18841 gcccgaaggg cgctagtttc gtcagactgt cagctaaact aagggtaatt tctcgagcgc
    18901 ttaaagcgga gtctaaagat aatttgggca ctaaaatttc aggagtcaac ttttttttgg
    18961 cgataacaag gagcttatct ctaaaaccat taagattttc cacgttctct atcgaaaagc
    19021 cacaggccat cggatgaccg ccatatttat ctaaaaattc tgaacattcc tcaatcgcgg
    19081 caatgagatt aaagccctca atcgaacgtc cagatccttt aaaagaaagc tgagcagctt
    19141 ttaagccggt gtctgcctgg ttcctttcct cctcttcaat tatccgggcg accaccaaag
    19201 tggggcgatg atatttatcg caaatcttac cggctatcaa gccaacaatg ccttcgttcc
    19261 aagactggcc gtcttgagcc acaccgataa taagaggcgg caaattatct ttatcaatct
    19321 gtgcctccat ttgcttaata atttcttcgg taatctgctg acgattgaga ttgcgctgat
    19381 ttaattcttg agccaaattt ctggcctcaa cttcatcttc agcgctaatt aaagcaaaag
    19441 cgctgttggc gtggcccaaa cgactagccg cattcaaacg cggaccaagc tgccagccga
    19501 cattccattc ctccaaagga cgatctaaag caattttggc ggtttgaaaa agagctttta
    19561 agcctaaacg tctattctga tttaatatgg atagacctct tttgactaag aatctatttt
    19621 caccaagcaa gctgaccatg tcggcaattg tcccgaccgc caccaaatct aatgatcttt
    19681 cggcgattag aaatttttgc ttagcgggta aagtagcttg gtataataaa gcgcaaacaa
    19741 gtttaaaagc tacgccgaca ccagctagaa agggccaggg atatttatct tcggcgtcgg
    19801 ctggatcaat taccggacaa gcgggcaaat cggaacgatt ctcgggaaga acatggtgat
    19861 cggtaataat cacttccagt cccaagcgat gggcataatc aacctcatcc ttattacgga
    19921 tgccattatc aacagtaatt accagactaa ccccctgctc ttttatttgg tctagagccg
    19981 atttattcaa gccataacct tcgctaacgc gatcggggag ataaacatcc accatcgcct
    20041 gtagggtttt tagggcttct gataaaacaa cggctgcagt gacgccgtca gcatcataat
    20101 cgccataaac cactatctta tttctcgcct tgatgtgctt aacaataata tcaaccgccg
    20161 cctgcatatc acgaaataaa aaaggattat aaaaaaccaa atctttattg ccagttaaat
    20221 caagggaaag ctctggctct aaagcgtcac ttaaaaaagc acgagcgacg gtcgcgctgc
    20281 gcaagcctcg attccataat atttgagcga tcaccggttc gatttccggc aaggagctaa
    20341 aaaattctgg aggcgccact gggtttatct gccaatattt ctgcatataa tcttaaaaaa
    20401 acatcgcacc aaaagtccaa atcaacattg acaccataat tattatggac ataatcgtcc
    20461 aagcgatttt taccgccttc ttccttttca taaaaaatat taatttagag atttaagatc
    20521 aataagccaa accttaaatc ttgtgtttac ctttttaaaa tcttcacaaa aacatcggaa
    20581 ggaataacaa cactgccttt accggccgcc atcatttttt tcttaccttt cttctgtttt
    20641 tccaacaatt tacgcttacg cgtcacatcg ccgccgtata atttagcggt tacatcttta
    20701 cgtctcgcgg atagacgctc ggcagcgaca acttttccac caaccactgc ttgaattttc
    20761 aaaacaaaca tctggcgcgg cagagtctct tttaatattt ctactatttc cttgccttga
    20821 cgataggctt catcccgata aacaatactg gcaagagatt caaccggctc ctccgcgacc
    20881 ataatatcta atttgaccac gtcagtcgga cgataatcta aataatcgta gttaagggaa
    20941 gcatacccgg cggacacact ttttatctta tcataaaaat cagtcaaaat cgcggataga
    21001 ggcatatcat aatgtaatat cgctctcgcc tcatcgctgc cggtagaaat atattcagta
    21061 tttttatata ttccccgttt atcccgcgac aaactcataa tattgccgag atacgcttgc
    21121 ggggtaataa tatcaagctt gacccagggt tcttctattt tctcaatttc actgggatca
    21181 ggcaagcact gaggagtgcg gacaataata tcttccccac ttttctttaa gacccgataa
    21241 gcaacgctgg ggacggtaac gattaaatct aaattatatt ctcggcgcaa acgttcttga
    21301 aaaatctcta aatgtaataa ccctaaaaaa ccgcaacgaa aaccgaagcc gagagcgtct
    21361 gacctttctg gttcatatga taaggcggca tcgttcaatt ttaatttctc catcgcttcg
    21421 cgcaaatcct cccaatcatt accctctctc ggaaaaacac cggcaaaaac catgggcttt
    21481 acttctttat agccggctaa agcagggact acttccctcc cagaagctaa agtatcgccg
    21541 acgcgacaat cgccaacttg cttaaaacca gtgacaacat aaccgatact accagcctga
    21601 agactgccgg tagaaaaata ttcaggtttg aaaataccaa catctaaagc ttcactcttg
    21661 gctttggtag ctgataaaac tatagggtct ccctttttaa tttcaccgtc aaaaactctg
    21721 ataaaggcga ccaccccttt atactcgtcg tacttggaat caaaaattaa agcccgcccg
    21781 acagaagcgg aagaagcccg cggcggcaaa ccccgggtaa taacggcgcc cagaatttcc
    21841 tcaacgccct ctccggtttt tccggaagcg caaagaatct ccgattccgc cccgccaagc
    21901 aaacgactaa tttctgtttt tgttttgaaa acattggcgg ctggtaagtc aattttatta
    21961 actaccggaa taatcgttaa atcctgctct aaggctaaat ataaattagc cagtgtctgc
    22021 gcctgtattc cttgagtagc gtcaaccaac aaaattgctg tttctactgc cgccaaggaa
    22081 cgggaaacct cataagaaaa atcgatatgc ccgggagtat caattaaatt cagctggtaa
    22141 ccttgatagg tcatcgtcac cggctgcagc ttaatggtaa ttcctcgctc cctttcgatg
    22201 tccatgccat cgagaatctg atttttcatt ttccgcttat caaccgtatt ggtaatttcc
    22261 aacattctgt cggctaaggt cgatttgccg tggtcaatat gagcaagaat ggcgaagtta
    22321 cgtattttat gcagattatc attcatgtcc gtaaattata aaaactaata ttaaataaaa
    22381 atatcctaaa aattggattt gtaaaaatac cttatcatta ataaaaaaat cgggcaagag
    22441 gaaaattaga gaccgcgagc ctccccttga tcagctaatt tcaccttttt taccgataaa
    22501 cgcttcttaa aaatggcaat agccgacaat aattcttcac tgcgaaaaag atgacaaaga
    22561 cagatataag cgataacgcc gccgctagcc gccactgaga actgaataaa aacgccgaca
    22621 aaagtcgtca tatcaataaa tggccagacg gctatcttta aaatttggat aaccaaaccg
    22681 gcgccgatag cggcgacgga aaattttaaa accgatacca aaattttatc caaatctaaa
    22741 gagccaacgc ggaaatacaa aacaatccat aaacttaaaa aattaacgat actggcaata
    22801 gaaaaagcca gcgccaggcc cgctactccc aaataaggat gtaaatacca agcgcaaaca
    22861 atatttaaaa ctacagaaaa taatcctaaa taaaaagggg taagcgaatc gcggcgagcg
    22921 taaaacactc tcactaaaag gggtaaactg gcttgcgcaa acaaactaag agaaaaaaag
    22981 ccaagagtat tcatagttaa aattgtatcc tgccaattaa aagcgccact gcccaaaaca
    23041 agacgaatca actgcgccct gagggtgata agtagtacgg aagcgggaat aataaaaaat
    23101 aatatttgtc tgatggtcgc ggaaaatcta actttaagct cttctatctt ataagcattt
    23161 tccgataaaa agggaaaagc ggcgacggca aaagaaatac caaaaacacc caaaggaaaa
    23221 gattgtaaat tattagccaa attaaaaacc gccagagaac cgctgtccag taaagaagct
    23281 aaagcggtta tggctaataa attaatttgg gaaacagcta aagacaaagt tctcggtccc
    23341 atcatcatgg caatctggcg caaacctcgg tcacggatat caagtaaaaa acgatagtga
    23401 aaaccgagag accaaaccgt cggtagctgt acggcaaaat ggagaacggc tcctaatact
    23461 accccccaag caagtccatc tattccccaa aaatcaacca aaaccaaagc gccaaaaata
    23521 atgccgagat tatagaaaac cggcgataat gaataaacaa aaaaacgctt ataactttgc
    23581 aagatacccc ccagcacccc cgacatccct aaaaataaag gagataaaaa cataatgcgg
    23641 gtcaggtggg cggtttgggc tttagcctct gcgctgaaac cgggagtaat ccaggcagtc
    23701 accggggcgg caaaaataac gccaaaaaca gacagtaaaa taatcagtaa taacagagaa
    23761 ttaaaaacgt tgttggcgag gcggactaat ttttcttttt ctccaggcca aatttcagaa
    23821 cgtttaatca tctctgaaaa taccgggata aaaccagccg acaaagcgcc caaaacaatg
    23881 agattataaa taaaatcagg cacgcgaaaa gcggcgtaat aaatatccag ctccgctcca
    23941 gcgccgaaac ttccggctaa aatatgatcg cgcaccactc ctaaaaaacg agaggccact
    24001 gaaaataaag ccactagagc ggcggcggcg gtaatgttgt tgattggccg gccaattaat
    24061 ttacgcaaag acaacataag ctgataagaa taaataaatt attttacttc tgcctcactt
    24121 tgtagagcgg ttaaagcctg ggcagcgctg tctagtcggg gatttaaacg caaagcttca
    24181 ttccataaag cggccgcctc ctgataacgc cgatactggt aataaactct acccagatta
    24241 tataaagcta aatcatattg aggattaatg gctaattcct tttttaactc aatttccgcc
    24301 cgcccaaaat cgccgcgatc aatataaatt gcggccaggt tattgtgtgc catcggctct
    24361 gaggaattta agaataaaga ttggcgaaat cgcccttccg ccttttctaa atctccctct
    24421 aaataataca tcgcccctaa attacgctgg gctaaggctg aatgcggcga actgctggcg
    24481 gcttgctccc aaaaagataa acggtctcta aaactgcgag aatggaacaa attaatagtg
    24541 gctaaaacca ctaaaatagt ggctaaaaca taacggcggc ggcgcaaggg tagaccctgc
    24601 cagaattttt cagtttctgc caaaataata agcaagccaa acatcggcac atataaacga
    24661 tgctccataa aattacggaa aacatcgctg tctgggcgga tagaacctaa aactaaaaat
    24721 aaccagaacc aagcaaaacc gaaaagtaga cgctttcctc tccaaggacg ggaataaaat
    24781 aataatagca tcaatatcaa taaagagata atcccgtaaa tccagggcga atcagccggg
    24841 accggataaa cagataaatt taccggcaac acaacttttc ctagaaaaat tagaggcgac
    24901 caaagattat cacccaaaga ctgccaggct aagccagacc cagtgccccc gagagcaact
    24961 aggcgcaaca gtgcccaaag aaacattgac accagtgccc ccgctgatgc taatagcaac
    25021 ttaaaccaag taaagtaacg atattcaaaa acaaacatcc aagcgagagc aaacaacggt
    25081 aataatatag ctgtttcttt actcaataga gcgcttagaa aaaatagcca cagggccact
    25141 aaatcgccaa ttttttcccg gcggagccag cgcgaccaaa aaattaaagt cgcccagata
    25201 aacacagcca ccagaacatc attccggccg ggaatccagg ccaccgctgg cgataaaacg
    25261 ggatgaatag taaacaataa gcttaaaaat aaagcagttt ttcccctaat atcaattaag
    25321 cgcaatagat aaaacaacaa acaagcagcc acggcgtgaa aaataatatt gctcaaatgg
    25381 aaggcaaaga cagtcccgcc accccagtgc caatcgcaga ccaatgacca ggtcagcaaa
    25441 gggcgataat aatatttagc cgcactaaaa aaaacatcgt ttaaaaatat ttctgccgga
    25501 ttggctcttt ctaaaatatc ggcatgctct aaaattaatt gttgatcgtc aaaataacta
    25561 taatcgaaaa ataaactctg cccataaaca ataagggccg ctaaaattaa aaacaaatag
    25621 gcgtgccagg agcgccaatg ataagaagat ttaatttttt cccagaaaaa aaagcgcata
    25681 agctacggcc gataaccgaa aaaataaaaa atagctaaaa ataaagtacc gacaccgtaa
    25741 cgcaaaccgc ggcgccaatt aatagatgaa gcctcggaaa aataacgaac cggcaccgga
    25801 atttccccga tacgccattt tctttggaca atctgcagta atacttgagt atcaaaaata
    25861 aaatcatcgc tggttttaga aaaatctagg ctggataaaa cctcgcgaga ataagcgcgc
    25921 ataccagtat gccattctga taaattctgg cctgtagcca gattctccac aaaagttaaa
    25981 aagcgattgg cataatattt ataaataggc atgcctcccc gcaaagtttc ttgccgactg
    26041 cgaacgcggg agccaagcat cacgtcaaaa taatcatcag cgataaaaga agcgaaataa
    26101 gagaccaaac gaggatcata ttgataatca gggtggacca ttactacaat atctgcccct
    26161 ctttccaggg ctaaacgata acaagatttt tgattggcgc cgtatccccg attgcgctgg
    26221 tgcgcaaaaa caaataaacc gagacgctga gccagtttaa tcgtatcatc gctgctaccg
    26281 tcgtcgacca aaataatctc atcgactaca ccgggcaaat ccgccaccgt ctgctccaaa
    26341 gtggcggcgg cattgtaggc cggcaaaacc acgattattt tttttccttg atacataata
    26401 atgaaataat taggctcaaa agccagaaac cggctaatac cgagacattg aaataaagac
    26461 aaaaaaatcc tggcagcaga ctttcaaagg ccaaacccac cgccactaga gcgctaaaaa
    26521 aataaaaaat ctccttagct aataatttac atagatcatg agaaataaaa tattttttca
    26581 taaattcagc caatctttaa tcttcgcttc caaactaggg ccggaaaatt caacattaat
    26641 actctttatt tccaccaaac cgccaccccc accctctgct aataatccgg gaatagataa
    26701 aatcaaacta tatttccccc gctctcgata taaatcagag gcatcaaatt ctagggtgct
    26761 ggacaaccag ccttcatcct cgataatagc cggacgataa tcagccaaaa caaaatctag
    26821 ttgttcgttt aaagaagaaa aacggtctaa acgggggtaa tccggattaa ttaaggccga
    26881 ggaagaggcg gcaaacatgc cgttattagc taaaatcaaa gaaccggtac tcaatcttat
    26941 attttccccg cctaagcgct tacttttaat ctcatattgc ttataaatct cggttaactc
    27001 cagatgacga ccgccaaaat caagagtctg taaagccgcc ggacttaaag ccttcacctg
    27061 caaataagga gcatcggtta ctaaagaaac ggcctcttca gtaaaaggcc aaattttatt
    27121 aattattgat aaataggctg aatttacaga caaatcactt aaaactaaat cgtcccctgc
    27181 tcgaaattct aggcgataga gaccagaggg caaatcagaa ttaactattt ttacagattg
    27241 aataatcccg atgtcgccac tttcttcctt ttgcgggcgt ctatctggta aagacaggag
    27301 atcaacttga ttcctgccag aaaaaactac aatttctgcg tcatctatat ctttattttc
    27361 gtttaaatct aacaaattac cagatagact taaaccctta tttttaagaa atatataaaa
    27421 ttgatgctcc ccgcgaaggg ggtaagggaa aaaagaattt tcagacactg gagacaaaga
    27481 acgtaagtct aaagcggggg gcgcttcctc taaagataga ttgtaaaccg ctaaacaacg
    27541 aggctgagaa caaaaagagg agccttgact ccgccaaaca cttaaaaatt ccgacagcga
    27601 agaaaatttt tccttcctct ggaataaact aagattacca gaccggagct gagtccaaga
    27661 agacaaacct tgctccagcc ataaattata aaccggctgt aaacggtaac gccaaagttt
    27721 tttatccgct aaaaaaccgg cctcaaaaat cggcgtagag gaacttaaat gaggacgata
    27781 agtaatcttg atggtgatgt gactgaaagg tctagggcta aaaacagaaa aatataccgg
    27841 ttctcctaaa attaacaaag gcccaccctg agttttaatc accctttcct ttggagtagg
    27901 gtcgcccagg caagaagaag cttgaaaaga agaaaaagaa tgggtacaag accaatcgcc
    27961 gcttggggaa acaaattgat aagctaaaaa aataataata aaaaacaata atatcagcca
    28021 aaaaaaccta atttttttat ttaaaggcat cttcataaga taataaatat ctggcgcttc
    28081 gatcttttca aagctcaagg cttatcctta ttatactaag attctctaaa aattaacaga
    28141 taaaaacaaa aatccctctg attatcggag ggatttttat cttaccaaat attaataaat
    28201 tttagttttc actaactgct ggcggttgaa tattttcata tttgtcgcgc acttttttgc
    28261 ctttataacc agtaccggcc ggaattaaac aaccaataat aacattctcc ttcaagccct
    28321 caagataatc aatcttgccg ttaactgccg cctcaatcaa cactctgggc gtctcttgga
    28381 aagaagcagc tgataagaag ctgtctgtgg ttaaagaagc tttagtgata cccaacaata
    28441 aacgatcggc tttagcttta actttggcta cctgattggc gtgttcaaaa acttccgcct
    28501 ccacggtttc accgggcaac atactggtat caccagggtc tttaataaga tagcgagaga
    28561 acatctggcg agcaattatt tctacatgct tatcgtttaa aggctgacct tgtgaagaat
    28621 aaacatattg tatttcccgg ataatatatt tttgagtctc gagttcgccc tttaatttat
    28681 acaattcgcg taaatcaagg cttccctccg ttagttgtgt tcctttttct acctcatcgc
    28741 catccttaac catcatcatt gtccccttta aaatagaaaa ttccttcacc ttgtcgacat
    28801 ccttggtaat actgacatac ttatcattaa ttgacactac gccggcactc tttgctttta
    28861 cgctttcacc ccccacctta aaaatctcta cccccttcgc gaccttatcg ccatctttaa
    28921 cggaaatttc cggaattaat tttttcttgt ctttaacggt caaacccgaa gctaatttta
    28981 actcattaat tttctcggaa aaataatatt tctcctgttc tttgccgcga taaatgatac
    29041 ttaaaatttt cgcttgcgga ttggtaatga cgatatcttt gccgtcttga tctttaatcg
    29101 tcttttgggc aaattcaatc ttaaccctac cggcaacgtc agaaattaga gccttcttct
    29161 tcgggctgcg cgcttcaaaa atttcttcaa cgcgaggaag accttgagta atatcgtctt
    29221 gtccggcaac cccacccgta tggaaggttc tcatcgttaa ctgcgtaccc ggctcaccga
    29281 tagactgagc ggcaataatg cccaccgccg cacccagctt aacctcttta ttataagcta
    29341 aatcccaacc ataacactta gcgcagacgc ctttatgtaa atgacaactt agcaccgagc
    29401 gtagattcaa agcactaacg cctttcgcct tcaaatcctt agatacatct tcgttaatta
    29461 aagacccggc tttcaataaa actttattct tatcatcaag caagtcttgc gccaaatagc
    29521 gaccaacgat ttttttataa aaatcttcac cagttttttc tgtttcttcc ttgatataac
    29581 atacgccctt cttgtcgccg caatcatctt cagtaacaat cacatcttga gcaacatcaa
    29641 tcaaacgacg agtgagataa ccggcgtttg ccgttcttaa agcggtatca gacaaacctt
    29701 tacggacacc gtgggtagaa ataaaatatt ccagcacgcc aaatccttct ttaaaattcc
    29761 ctttaaccgg taattcgata atagcgccgg aaggtgaagt taccaaaccc ttcatgccca
    29821 aaatttgaac cggctgagcc caagaaccac gggcgccgga atcaatcatg gagaaaaccg
    29881 gcaaaatatt aactaagcct tccttgcaaa tatcggcaat ctgatcttta gtgttggccc
    29941 acacttcaat aaccttggta tagcgttcac tctcggtcaa taagccgtct tcatactgtt
    30001 cttgaatgac atcaacctta tcattggctt cggcaattaa gcggtctttt tccggcagat
    30061 aaggcaggtc accaaaaccc caagaaagac cggatttagt gatgaatgaa aaacttttat
    30121 tctttaaatt atcaataaat ttaaccgttt cgtcttccgt ataaagacgc agacattcgc
    30181 ggattaaatc ttttaacttg cccttgccga ccacttcgtt gatatagcgc agcttttcag
    30241 gcaaaatcaa attaaaaatc atccgaccaa cagtggtgcg aagacgttgc ccgttaattt
    30301 tagcgatcac cggctcgttt aaagaaacga aattatggtc ataagctaaa atcgcgtcat
    30361 cgtcattata aaaatatttt aattcctgat cttttttgtc tttataggcc ggatcttcca
    30421 gagtaatgta ataagttccc cagacgatat cttgagcagg tgccacaatc ggatcgccag
    30481 tagccggttt taataaatta tgggaagaca gcatgatgtc agccgcttcc tgcttcgcct
    30541 ccttggttag cggcacatgg acagccattt gatcgccatc aaagtcggca ttaaaagcag
    30601 tacaaactaa aggatgaatc tggatggctt taccctcaat taaaaccggc cggaaagcct
    30661 ggatgcccaa gcgatgcaga gtgggggcgc gattcaacag cacataggcc tctttaacag
    30721 tttcttcaag aatatcaaac acctcatcat gcccagcttc aatataacga gaagcgctgc
    30781 ggacattatg gacaatctcg cgtttaatca actgactgat aataaaaggc ttgaacaact
    30841 ccaaggccat gattttaggc aaaccacatt gatcaaggtt taacttagga ttaacgacaa
    30901 tcacgctacg gccagaataa tcgacgcgtt tacccaataa attctgacgg aaacgaccct
    30961 gtttcccttt caagccatca gctaaagatt tcaactggcg cttttgtccg gttgaggcgg
    31021 tcacggtctt ggtgtgacgg gcggagttat caatcaaagc gtctaccgct tcctgaagca
    31081 tccgtttttc attacggcaa attacttccg gggcattcaa atcaatcagc tgtttcaaac
    31141 gattattacg attaataaca cggcgataaa ggtcgtttaa atcagaagcg gcaaaacgac
    31201 caccatcaag agcaaccatc ggacgtaaat caggcggcac taccggcaag acagttataa
    31261 tcatccactc tgggcgtaaa ttattattac gaaaactctt taaaagcttc aaacgtttag
    31321 ttaatttatc gcgcttagaa tcgacggcat cctttaattt ttcttctatt ttttcaatac
    31381 tcttagctaa atctatacga gaaagaagca atctaatagc ttccgcgccg atgccggctt
    31441 caaataaatg accaaatttc aagcttaaat tttgatactg ctgctccgat aatattagta
    31501 aaggcttaag ttctttcaat tctttagaaa cttgaataaa gtcttcctcc agtcttttta
    31561 atttttcatc gcgttcagcc gtggcaactt taatctgatt agcaatactg gcgtcaacct
    31621 ccgccttggc tttaccctcc tttataatat cagcccgact gtcttgaatc cgcttaatct
    31681 gctgaactaa ttccgcctca atgctcttgc gcttagattt atgttcgttt ttaatctgct
    31741 ctaaagcagc ctctttcaag ccttcatcaa catcagtcac gataaagctg gcaaagtata
    31801 taaccttttc caaagactga ataccgagat ttaaaagtaa accaattttt gaagaaattc
    31861 ctcttaaaaa ccagatatgg gtgcaaggaa tctggaggtt aatgtggccc atacgctccc
    31921 gacgaacaac actgcgagta acttcaacgc cgcacttgtc acagacaata cctttatagc
    31981 ggattttctt atacttaccg caagcacact cccaatcctt ggtcgggcca aaaatctttt
    32041 cgcagaaaag tccgtctttt tccggcttct gtgtgcgata gttgattgtt tccggcctaa
    32101 cgacttcccc gtgcgaccaa gaaagaatct cttcaggcga agcgagcttg agacgcaagg
    32161 cgtcgaaatc gctagtttta attggattca acatatatgt gatagattga tttgataaaa
    32221 tttacagcga aaacttattc ttccgtcggc agggcagcct cgacgctatc ggaagtagca
    32281 attatctctg gtttgtcgta gtcataacgc ggatccttct ttttctctaa gtcaatctct
    32341 gtttccactt ccgaggcagt ctgaccgtta ctgccgccca aaagctcaac actaagacca
    32401 agacctttaa gttcccttat taacacgtta aaagactcag gaacgttaag cttctcaatt
    32461 tcttcgcctt taataatcgc ttcataagcc ttactgcgtc cgggaacgtc atcggatttg
    32521 atagtaagca tttcctgtaa agtatgggaa gcgccataac cttctaaagc ccacacctcc
    32581 atttcaccaa aacgttgacc gccaaactga gctttaccgc ccaaaggctg ttgagtaatc
    32641 aaagaatagg gtccgattga acgctggtga atcttatctt ccaccatatg attaagcttc
    32701 aagatatact tataaccgac ggttatttta tggtcgaaag cttcaccggt tttaccgtca
    32761 taaagagtga ttttaccgtc ctcgggtaaa ccggctttct ttaattctct cttaatttgt
    32821 tcctccgaaa caccatttaa agccggagta gcgatgcgat agcctaattt cttagcagca
    32881 aagccgagat gggtttccaa taactgaccc aaattcatac gagaaataat acctaaaggg
    32941 gaaagaataa tatcaatcgg agtgccgtcg gccatataag gcatatcttc gaccggaact
    33001 actttggaaa taacaccctt gttgccgtga cgaccggaca ttttatcacc cacctgaatc
    33061 ttgcgcaggt tagctaccga cacctgaatt gatttcaaaa caccagccgc caacctgtct
    33121 ccctcttctc tggaaaaaat ctttacatca accaccttac catgttcgcc gtgttccaaa
    33181 taaagagaag agtcccggac atccttggcc ttttcaccaa aaatggctct tagtaatttt
    33241 tcttccgctg acaaagttgt ttctccttta ggggtaattt taccaaccaa gatatcgcca
    33301 gaagaaacct ccgcgccaat atggataatc ccatcctcgt caagattttt gagcttctcc
    33361 tcacttatat taggaatatc attggtaatc aattcggggc cgagcttggt atcgcggaca
    33421 tcgattgtgt agttttcaat atggattgaa gaataaatat cttctttcac taagcgctcg
    33481 gaaataataa tagcgtcttc atagttgaag ccatcccaag tcatgaaggc caccaagaca
    33541 ttacggccga gagagagctc tccgttatcg atagaagggc catcggttaa aatttgaccg
    33601 gctttgactt tttcgccaac agtcactaaa ggatgttgat taatgcaggt ggaggaattg
    33661 gagcgtaaaa atttatttaa atgatactgg cttatttggc ctttcttatt ttcaatttca
    33721 atcaaagccg cctcggattt aactacgatt ccgtcttctt tggcaatcgc tacgtggccg
    33781 ctatcgcggg cggcacgagc ttctacgccg gtaccgacaa taggcgcttc cgcttttacc
    33841 aaaggcacgg cctgacgttg catattagtg cccattaaag agcgctgtcc gtcattatgt
    33901 tccatgaaag gaattaagga agtagcaata gagataatct gattagcggc gacccccaca
    33961 aaatcaattt tgttaacatc ttcaactcca ggttgaccaa acttacggac ctcataacgg
    34021 ctaacggcca aatgcccgga agcatccacc ggaatggtgg cgtcagcggt gatatatttt
    34081 tcttcctcaa aagcatctaa ataaacaact tcgtcggtga ttctgctgcc actcttttca
    34141 tgtttaaccc ggcgataagg agtttctaaa aagccataac tgttaagacg ggcgtagcta
    34201 gccaagtgac cgaccaaacc aatattcggt ccttccgggg tggcaatagg acagatgcga
    34261 ccatagtgag tggaatgaac gtcgcgtaca tcaaaaccgg cgcgctctct ggtcaaaccg
    34321 ccagggccca tagccgataa gcggcgctta tgctccagtt cggccaaagg attagtctga
    34381 tccataaact gagacaactg agaagacatg aaaaactctt taacaacacc aattaccggt
    34441 cgtgaattta ctaacttatt gggagtcaaa gtagcaatat ctgaggtgga catcctgtct
    34501 ttgataatac gctccatccg tgccaatccc acccggaaac gattctgaat caagccgcca
    34561 atagcgcgga tgcgacgatt gcccaaatga tcaatatcgt cttccggctc tttagttata
    34621 ttcaaacgaa taacttcctt tactattaaa agcaaatctt ctttgcgcaa aacccgattc
    34681 tcttttttat ttggcaaatc caaaccaaag cgacgattta atttatagcg accaacacga
    34741 ccaaaatcat agcgatcaaa acggaaaaac atggaatgga taagttgacg agcattttct
    34801 acagaagcta aatctcctgg gcggatgcgg cgataaacct cctgcaggcc ttcggcttca
    34861 ttcttggccg cgtccttctc aatcgttgct tcaataaaag taggctcatc attgctctta
    34921 accaaatcaa catcacggaa aagctttttt aattcttcat cattttcata gccaaaagca
    34981 cgcaagagcg acgtcaccgc cactttacgt ttacgatcaa tgcgcaccca aattacttta
    35041 tttgaatcag tttctatttc cagccaagca ccacgattag gaataatctt agcgccgtaa
    35101 cattttttgc ctttaataaa ttcagcggta aacatcacac cagccgaacg aatcaactga
    35161 gaaacaacca cgcgttcaat gccgttaata ataaaagtgc ccttatcggt cataattgga
    35221 aaatcaccta aataaacttc ctgattagag ctttggttgg tcttcttatt taataaacga
    35281 gtcttaacgc gcaggggagc ttcataggtg atgttcttgg cacggctctc ggtttcggaa
    35341 aatttaggct catcaagata ataatcttca aagtataatt ccaaatctct gccaatgaaa
    35401 tcggtaaccg ggttgatttc gtcaaataat tcagccagcc cctcctctaa aaaccagcga
    35461 taagaatctt tctgaatttc aattaagtca ggcaagggca tgacctcttt gaaatcggta
    35521 aaaaacttac gctctgcgat ttttttatcc gttgagctta cgttagacat agaactttaa
    35581 gggcggcaaa ccaagcgcca tctgccaaac agaaaggcga tagtccggcc tgaatttatt
    35641 gacttgatga agataggcgc tttcaacaaa aaaatctcaa actgatttaa gcttgagtta
    35701 actgttgttt tatttaattg cgccgaaatt ttataatcac tttagaatat gcttatttat
    35761 aaagtgaatt ttggctaaaa atacaaaaaa aacggattgg tgaataaaga aaaaataaca
    35821 cctgagcaac cgtttttatt atcatttttg cctaatagct gattgtatta tctatcatat
    35881 tataataatt attttttgtc aatacacaat ttctccacag aaaaaattaa gcctcgacca
    35941 gtcaaaaaac taaactatta tcaataatat gctaattctt cccgagcaac cacacggtca
    36001 tcccaaggaa ttcgactacc tctgggaccg caaatatact gctcggcccc tttaccggta
    36061 attagaacga gatcgccttc gctggccaga cggaaagcca ggcgcatagc ttcgcgccga
    36121 tcaagaattt tataaagatt cgattctaga attttccctg ccttttctgc ccccgccgcc
    36181 acttgattaa taatatcctg aggattatcg tcatatggat cttcattggt cacaatcact
    36241 atatccgctt tttctccagc cattttacct aaaattgacc gacgagcctg atcacgaccg
    36301 cctccagccg agcctaaaat atgaattaag cggcgataat gaataaaatt aaggttttga
    36361 tacaaggcct ctaaggcttt aggctcaaag gcataatcaa tcaaaacatg aaaattttgg
    36421 ccagcctcta tcttctccat cttgccggct aaagatttaa tatttgccaa agcggccgcc
    36481 gcttgttcag ttttaatatt aagcacagaa gcgactgtta aagcggcaac ggcattaaaa
    36541 acatggtaag acccgagaat cgaaagatga cagcgctggc cgttaaattg aaaatcagaa
    36601 cctgaggcgg tggcgaataa aggctcataa agaaaaattt ctggtggcgg ttggatctcc
    36661 tgcccagagt catccgttag gcttgtcgcc accaaattcg gattaagcgt atatacgcat
    36721 ttacgttcgg ccgggaaaga taaaaaatat gaagcctgat catcgtcgcc atttacaata
    36781 attgtttttt taacctttaa aagttcggtc tgtttcaatc cgctcgggtg agaaactacc
    36841 tgattattat catctaaata tttagagcgg caacggaaaa gatgagaaaa tagcttaccc
    36901 ttagcacgtt tgtagttctc aaaactgcca tgggcatcaa tatgttccgg atagagattg
    36961 gtaaaaacca ataaatcata gttaataaaa cgatgacgga actgctctat cccttgagaa
    37021 gtggtttcta taatagcggc ttgacaaccg ttattaacca ttcgccgaag agtccgttgt
    37081 agaaaaaaac gaccgggcat tgtcattttc ttatcgttta accactcctt ctcgccatca
    37141 ttaaattgag cggtagaagt ataaccagct ttataaccat tagcattaag aagcctagac
    37201 aaaaaataaa cgcttgaagt tttaccggtg gtaccggtaa cgccgatgat aagcatttgc
    37261 tgactgggaa aacgataata caaagctgcc agatagccca taatgaaatg ataaaagggc
    37321 tgtaaactct ggaaaatagt ggttggtatt aaacgtctaa ttctggcaag catatctaat
    37381 tagtataaca agaaactaaa aaagaaaaaa ccaaacaatg gccttctccc cgcgctcgct
    37441 agcggctcat gccgggtttc ccgacctatt aaaaaaggcc aaaattgacc tttttgttta
    37501 atcgtttacg ctatcggcta aaaaacatcc cttcgtcatc atcatcgaag acacgagact
    37561 taagtgtctg ttttttttct tcagcctgtc tgggtggcga aggctcctcc ggcttgattt
    37621 cctcaagaat ttcgttctcc tcctgaacat cgtcttcagg gccgttgtca tccaataaga
    37681 aagcgggaac gttttgatta ttatcttcat cctcttcggt tttgctttca atttctgtct
    37741 cttttaccac ttccttgtct tcgtccccgg atttaaaaga taaagtatca tcatcacctt
    37801 catcattccc aaaataatgg tcgggagcgg acatttcgtg acgattatgt acgggcatcg
    37861 cgctaattcc ctgattctgc atattttcta actgttcctt gctgattaat atttctcttg
    37921 gctttgaacc gttagctgga ccgatgatac cggattcttc cagcatatcc aggatcttag
    37981 cggctcgccc ataaccgatt gacaaacgtc gctgcaataa agaagtagaa gctttgcccg
    38041 ccttaataat tatttcttga gcttctgaca ttaattcgtc ttcatcgccg taagtaccgt
    38101 caagaccgac accggccaca ccccccacct tttgtctgtc ggtcacaccc tcaagataat
    38161 cgaccgcgcc gctcttttct ttaatataat taatcacgtc attaatttct tggtcactga
    38221 cataagctcc ttgaatgcgc ttcggcttgg aaagctcagt tgtggtcaac aacatatcac
    38281 cctgacccag taatttttcc gccccgctca tatctaaaat cgttttagaa tccactaaag
    38341 aagtgacgga aaaagcgata cgagcgggta tattcgcttt gattaagccg gtaataacat
    38401 caacgctcgg tcgctgagta gcaagaatta agtgaatgcc aacagctcta gacatctggg
    38461 ctaaacgaat aatggccgct tccatttctt tgccagatgt catcataaaa tcagccaatt
    38521 catctatgat aaaaataata taaggcaatt tctcctctgg cttatcggcg ttataactct
    38581 ggatattttt cttgccggct ttattaaata aatcatagcg cctttccatt tcatttagac
    38641 accactttaa agcgttgatt gttttggaga cttcggtaat aaccggcgtc aacagatgcg
    38701 gaataccgtt atagaccggg agttccaccc gtttaggatc aaccataata aagcgcaaat
    38761 catcggggtt gttttgataa agcaacgaaa taataatcgt attcaaacaa acagattttc
    38821 cagaattagt ggcaccggcc accaataaat gcggcatgcg cgtaatgtca taaatccaaa
    38881 ccgtaccgga aacatctttt cctaaagcaa tcattaaatt atgtttacga ttcttaaaga
    38941 tagggtcttc taaaatctcc cgcaaaccca ccacggcttt gactttattg ggaacttcaa
    39001 ctcccactaa cgattgaccg ggaatcggcg cttcaattct gattggatgc gccgccaaag
    39061 ctaaagataa atcattattc aaagtaataa tacgtgaaac ttttacgcct tcagccggct
    39121 taaaagtata ttgggtgact gtcggaccga cttttgtttc cgccatttct acagcaatac
    39181 cgaaattttc caaagtgcgc ttaattaaag ccgcccctct ctgaatatca ccgccaaccg
    39241 cctgaccaga tttatgattt aacaattcaa tcggcaaatc tatttttatt tttttcttgg
    39301 gcaaagataa tttggcctga accttagaag tggccgcctg agccaaatta gcctgcttag
    39361 ctgacaattc ggaatcaatc ttatcctcag cgtcttcatc cgattcgctt tcagctaatt
    39421 cccgcttgcg ccaagacgcc aagacttcag actgggtaga ctcaatagta tcttccgctt
    39481 ccccctttcc cgctctctcc gcagaggaag cagaaaaaac accgcgaaaa gcattgccta
    39541 aagtacgacc gaaattagcc aaaaaacttt cccgaccgat aagtttagac agagaggtat
    39601 taaagacaat caccaaagct actaacagca aacagaataa aattatcacc ccgccccaga
    39661 agccaaaagc gtaaagaaat aaacggctta aataatagcc gatataaccg ccgccaagac
    39721 cggcctgagc attgcttagc caatcggctt cttttaagaa aaaatgaaac aatgtctgat
    39781 aagaaataaa taaaagaaac aagcccaaat aatcactcgg tttgacatca accgtcttat
    39841 tgcgcaccag taatatcccc caaaaaagca aaactagagg cactaaccat ttgccaaatc
    39901 caaaaattaa agaaagccaa tgtgacagaa aaacaccgga acgcccagaa agattgaata
    39961 agcctaaaag agaaattaaa ccaaaagcaa aaacaataac gatgccgata ctatgtttag
    40021 cgtggtcgct taattcaaaa ttaggtaaat tttcgaatgg gttagccgtt tttttctttt
    40081 ttttacccat aaaattaaaa taattgccaa taaaggcgcc ggcggactat cttcattata
    40141 caaaaagctt tagtaaatct caaatagccc aatcaatatt aattaatctc gatatcaata
    40201 aaaactatct tcggaaaacg ttctctaatt tttttctcga tttcatcaat ctttctgctc
    40261 actaaacgcg gctagggcca gcccgaagcc cgaggaagga gaggacgaga tcttttaaaa
    40321 ataataaaat tattacagac tattatacaa atctaaatac ctgggtacag atgcttgcca
    40381 agaccaatca gctgacatgc cggcgcgacg aagtgcttga tatttatcgg gctcttgata
    40441 atagagacgc aaagcccttt gcagtgaaga caacaaggaa gaatctgtat aatttttaaa
    40501 gacaaaacca ttataaccgt caataaccgt atcctttaga ccgccagtcg ctctaacaat
    40561 cggcaccgaa ccatatctca tggcaatcat ctgcgtcaag ccacaaggct cgaaacgaga
    40621 aggtactaaa aaataatcgg agccagcgta caaattacga gctaagacat catcaaaagc
    40681 aatttcggct ctcactgcct caggcaaatt aacagctaag gacgaggcta aactttcaaa
    40741 ttttttttcg ccggtaccaa ggattattaa ttgaaatgga tattttttaa tcaaagtttt
    40801 taacttgtta agattaaaaa ggtcgattcc cttttgatcg gagaggcggg cgactaaagc
    40861 caacagcggt atcggagagt cagataagcc gaaataagag cgcaaggcct gtttatttat
    40921 ggccttcccg gcgactaaat taatacgatt ataattaaaa gctaaagctt tatcggtagc
    40981 cggattataa gaaacaacat caaggccatt taaaattccg actaaatcat tttggcgaga
    41041 aataagcaat ttctctagtt cctgcccata ctttttcgtc attatctctt gggcataact
    41101 agggctaaca gtcgtaattt tatcagccgc cataatacca gccgctaaaa tattaaccca
    41161 attgccagcc ttattttttg ccgccgccgg cacaatatca ggattaaaac caaaagattt
    41221 aaatttagaa gctgagaccc aaccttgatg ttgtaaatta tgaatggtta aaacaagctt
    41281 cggccgggga taatctgact tctgtaaata agtaagtaaa actccggcag tatgaaaatc
    41341 atgcgcatgg ataatgtctg gagtaaaagc taaaaaaggc atactagccg ctaccgcctg
    41401 ggaaaaaaat aaatatttat ccaaagtgtt ctccccatac accgctccgg aaaaataatt
    41461 tttactttgt aaaaaataat aatgaacccc ctctaccttg gcgctcagca aaacgactct
    41521 ctcctttttc ttaccaaaat ttacggaaaa tgaggctaat ttcttagccc gccagaaacg
    41581 cagagaaata gagccatgcg ctggcataat gacgcgcacc tccactcctt gtttttttaa
    41641 ggctggcggc aaagaatcga caacatcggc caaaccgccg attttagcta aaggtgcggc
    41701 ttcagccgcg caaaaaagaa tttttttctt aatcttgagc atgatgatta gtatttaaat
    41761 aattttttac caaagcaatc atcgcctctg ctttttctgg ttcacattca tgctttaata
    41821 accaaaaagt cgcggtcctg gtggcggtcc agttgtaata taaatctaag cggcgctgca
    41881 gagattcatc caaagaaatg ttggcggctt gcaaataaga atttaaaaat agtcttttca
    41941 tggctagggc aaattcatca tcctgtaaat ttcgctttat cttatattcc agctgctgca
    42001 taaaagcgcc gaaatcacgg gcaaaatctg ccaaacaaaa atcggtgaaa tcaatcaatc
    42061 cgattttatc ggcgccgaca gcaataacat tttccggatg agcatcgcca tgaataagcc
    42121 aacgcttagt cgtgctggca aaaaattctt cctcctgttt aatgaaacga tcgtaaaaat
    42181 cggcaatatc gtggacatat ttttcttgaa aacgctggcc tatttcccaa acaatcaaat
    42241 ctcttcctgg aacaaccgtt cgcaacaagg aattatcctc tacaaatatc tctttggcgt
    42301 ttgccggtaa acttaaggca tgcaaccggg caaacaagcc ggccgcttgg actaccatag
    42361 attttacctt ctcacgatta tttaacttga taaaattcaa aagattctta ccttcaatcg
    42421 cccgataaaa agtgccgtta aattctgaat tgaaaaataa ggggcgcgga agagcgatgc
    42481 cgtcaccagg cagaccgctg ttccataaaa aatttaaaac tttaaaaaca ttctccctgg
    42541 cttcgtggct gtgagccgaa caaacaattt caatcttatg ctcctcgcca ttggctttag
    42601 ctagaaccaa gcgataagcg acaacaacat gataagtggt ggtccagatt aattttttat
    42661 aaggttttat cgtgaaactg acgatttttt caaactccgg ataaagcggt aacacttctt
    42721 tctctaaaag caaacgaacg gtctgtttgt caaacagact gaggatctta ttcataatag
    42781 ttgcccgaat ttctgacaat tcggcgatga atttatgtta ataaaaatta cgctgtccaa
    42841 aattggcgcc aatgtctttg ccagagacga gccctgatgg tagaaaccaa attttcggct
    42901 tttatttttt cttttaagct ccgtttatcc tgtaaagaac gaatggctct taataaatca
    42961 ttaatcccgt tctcaacttc atccgggttc caagccactg acccgaaatt atgaaaaaaa
    43021 tcgcggccac tggcccacca aaaagaacag ctagccaggc ctcggctcag atgccaacga
    43081 taccactgat aattcttatc gtcgccatat ttatcaccgg cggctatcgc cattcgggcc
    43141 aaacgccaca acaacctttg aatcttatta ttcgtatcat accagacaga aaagggatta
    43201 cgaccgtctt cgtcccaatt agtttcccaa gaagcggcac gaaaaataac cggtactaag
    43261 cttttctgag tatctaaaaa gtcggaaatg ttctgagtct gtaaattatc gagggcaact
    43321 attttttcca attctaaggt cggatcttca tgacgcaagc catatagctc ggcatccgta
    43381 gcggtaatca ataattcatg ggcgacttct gattctaata agtcgccgat aatatcgggg
    43441 gcataagcat tagaaaaacg ccgttggcgc acaattactt gcaaaccact attagcgtca
    43501 agataagcgc cgttaggatt acgctctaaa agatggggta atgaggcttc atcgataata
    43561 atccagcgat atccctgagc tttaactaat ttagctaagg ctggagtata ggccatttct
    43621 ggaaagaaaa atccgccacc gcgcacatcg acgccaagaa tctcttgcaa caactcctct
    43681 tggcgcttga tttgatataa cgcctccctt tccgggatta agggcaaaaa tgcatgatag
    43741 gcagccgaac ccaccacctc caggcggccg gacaaaatta aacgccgcca acggttaatt
    43801 aattcgataa aaccgtctgt ccgcaggcgt tctaaaaggc aagcgctgat attggcagta
    43861 aaacgcaaat cgggattatc ctccaaaagc tgagtgaggc gtaaataact tttttcaacc
    43921 gcttctttga ttctggcaga tgggctgtta gccggttgat aaagatgaag aaaatttaac
    43981 cacaacataa tttataaata tttataaatg tttaacccgg cgatatagct ggatatattt
    44041 tttagccggt atttcccagc tgttcgattg cttcattact ctttgcacta attttgtcca
    44101 agccgagcga ttgcgataat tttccaaagc acggataata gcccgaaaaa ggtcggccgg
    44161 atcctcacgt ttgaaagtaa aaccgttacc tcggccggta gccggattat aatcttccac
    44221 ggtatcataa aggccgccaa tttcgcgcac aatcggcacg caaccataac gcatcgcaat
    44281 caactgatta ataccgcagg gctcatggtt agaaggtagc aataataaat ctgaaccagc
    44341 ataaacttga gtctcatatt gctgattcgc tttatgagaa ggaataacta ctaatttttt
    44401 aggaaactta cgtgcgaatt ttcgcaattc actaatatat tttttgtcgc cggtgccgat
    44461 aattatcaac tgtacttcca aattaagtaa atcaggtaaa atttttaaca tcagttcaaa
    44521 tcctttctga aaagtgatgc gcgaagtcat actgatcacc gggatattgc ggccaatagg
    44581 caaaccaaaa aacttttgca aatgaaattt attctccgtc ttaccagata aattacgcca
    44641 agaaaaatta ttacttagcg tctgatcttt ttccgggtta tacgcataat aatcaatgcc
    44701 attaacgata ccaaaaagac ggtccgaacg attgcgtaaa atctggtgca aatcctgacc
    44761 gaactttttg gtcattattt ccgaacgata ctgttcgcta acagtattga taatatcagc
    44821 ggataaaatc gcccttttga caaaattaat tctttctaga cgagggtcgt ctaaatgggg
    44881 aatcggaccg cgtccataat ctttttcggc tgctggcact tcccaccaat tatgaccgaa
    44941 ctgaaaaact aaattatgga tggtaaaaac agtttttgca tttttcaggg ttttagaata
    45001 gcgaaagtca tgctttaaat aataaggaat caaacctgta tgccaatcgt ggcaatgaat
    45061 tatatcggct tcaaatttaa gtaaagataa aagcttcaag gcggccacat caaatactaa
    45121 aaaacgagca ttctcattgc tcgcaccata taaggcctta tgtttggaaa aatatttgcc
    45181 gcattcaata aaatatactg gcaaattttc taacaactgc ccctgccaat aattaacaat
    45241 aatggtttca tctgagttta agcgtaattt gacgttgcta aatatcaact taagtttata
    45301 ttccgtctta ttaattgatt tagtatacag aggagtaatt aaacagacgt catggccgag
    45361 acggcttaaa gacttcggta aactacgagc aacgtccccc aaacctccgg tcttggaaaa
    45421 aggcgcaact tcagaggcaa tatgcacaat ttttaaaatt tttcgcatag ttttagcgcc
    45481 actggcgctg attagccccg caaatagcca ccgccagagc atcagcggcg tcatctggct
    45541 tcggaatttc ttccaacttt aaaataagtt taaccatctt ttgaatttgt aattttgaag
    45601 cttggccata agcggataca gcccgtttga cttgagccgg cgtgaaatct aataaaggta
    45661 tcccctgctg gctgacagcc aaaagggcga caccgcgcgc ttgtccaaca attaaagccg
    45721 tgcggacatt cttgttaaaa aataattgtt ccaccgccgc tacctgagga cggtacttct
    45781 ccaacaataa acgtaattcc tgataaagcg actgaagacg ctccggcaag ggttgaccgg
    45841 caggagtctt aattgaacca taatccaggc attctaaatt tccgccctga acgcgaatga
    45901 agccgtaacc ggtatcggca atacccggat cgatgccaaa aataattttt tccatacata
    45961 gaggctcgga accagtagat gacgtattcc gagcctaaaa ttaagaagct atctattggt
    46021 tatattttaa tcagctaaat tagtgtaata ggcgctcaca tcctcattgt catcaagttc
    46081 ttctaaaaat ttatcgcact tttcctgatc gagctcgctg agattgactt tttctttagc
    46141 aacatattct atttccgcca attcagtctt taatttttta ctctttaaaa aatcctgcat
    46201 cttctgaaaa tcgtttaact cagtataaat agtcaaacct tctttggaaa cagaaatatc
    46261 gctagcgcca ttatcaatta aatccaattc taaatcggcg acgcttaaat tcttagccgc
    46321 caattcagcc tgagacaata aaattactcc cgcctgctca aagttccaca tcaccgcccc
    46381 gaaagaacca ccattcttgg taaaaatatg acgaatggta gaaacactac gattacgatt
    46441 atcagttaca cactttacaa tcacctgaat accggccgga ccgataccct cataataaat
    46501 ttcttcaatt tgctctcctg ctaattcgcc cgtacccttc ttaattgctc tttcaatatt
    46561 atctttgggc atactggcgg cgcgagcgcg atcaatcgcc gcccgcaaac tggggttagc
    46621 agaaggatcg ccgccggctt tggcggcgat ggaaataaca ttagctaatt tagtaaaaat
    46681 cgcgccacgt ttggcgtcaa caagctcttt ctgacgatgt gttgtcgccc acttggagtg
    46741 tccggacata attttcaaat taattattca cgcccccatc ttatcctaaa ttgaaataaa
    46801 aatcaatatc cgttataata aaaacatcaa taaaatcagg taaattatca aaaatatgaa
    46861 taaattggca ttagattctt taaacctcag tgtccttaag gctttagctt tcttccaaaa
    46921 aaatccaccc cctcgctttc agcttaaaaa ataccccttt cctctggtgg taggcagtgg
    46981 caacgccatt aatgtcggcc tggcgatctt cgaccaacaa aaagccattt ttgctaacga
    47041 aagtaacttc cgtttaatcc tgaaacgtta tcgggattta attaagcaaa aaaacattag
    47101 ccacgctgtc attatttccg cctccggcga aaaagattca gtctgggaaa ttaaagaggc
    47161 caaaaaagct ggcttaaaaa ccgtattgtt aacctgctcg cccgcttcca gggccgctaa
    47221 aattgcccat gaaacttata ttttcccgaa gctatctgag ccgtacactt ataatgtatc
    47281 gacctatttg ggcatgatta tggcggctac tggagaaaaa gcttcgacaa ttacccaatt
    47341 tatttttaat cttaaattgc caaaaaattt taaaaaatac cgcgcctatt cctttattct
    47401 gccagatgaa tggggagcaa ttgcgttgat gctggaaatt aaaaaaagcg agctcttcgg
    47461 tccgaatcta tccttacggg ctttttccta cggcgaatct cggcatgcta aatttgtcat
    47521 cagggaaaaa gacgagttag taatcagttt tggcaagaac aaatatttcg gacaccctca
    47581 acaccgctgg gaaataaaaa aacctaaaac ggcaaacaaa gcctgggcga tggccgtttc
    47641 ttatttctta atcggcctca tccaagatgc ccaccccgac tacttccgcc ccaaccttgt
    47701 taaattctgc cgagactacg gctataaagc ctatgctggc gctaaaccgt tcgacatcat
    47761 cgtgccagga aattaaattt aaaaatttac caaataacat gtcccctccc cgcgctcgct
    47821 ggcagcttgt gcgggatttc ctgatcttat aaaaatcatc ttcgcgaaaa aagatgattt
    47881 ttatttaatt ttattttttc ttgccttgat ttttaacttt atcgatagcg gcttttaatt
    47941 ctttggcgct ggccaaataa tatcttttaa ccggccgtaa atttttatcc agttcataca
    48001 ccagaggaat gcccgttggt atgttaaatt caacaatttc gttttccgac atagcttcta
    48061 aatatttcac caaagcccgc aaactattac cagaggctgt caccaaaatc tttttacctt
    48121 tacgtaataa tggtaacgct tcctttttcc accaaggcgt caccctcaag accacatcct
    48181 ttaagctctc cgccgatgga accttaatat cctgatagcg aacatcatcc ttttgattat
    48241 acttattttt attatcaatt tccggggggc gaacatcata actgcgacgc caaatcttta
    48301 cttgttcttc cccaaacttc ttcgctgttt gctgtttgtt taatccttga agattgccgt
    48361 aatgacgctc atttaagcgc caatccttta ttgtcggcaa ccataataaa tccatttctt
    48421 ctaaaacgat atttaaggtt ttaatagctc ttttgagaaa agagacatag gcaagatcaa
    48481 aactaaaacc ggcttttttt aattttttgc cggcagatga cgcctctaac aagccccgac
    48541 tgcttaaatc aacatcggtc cagccggtaa aaagaccttt ttcgttccaa acactttcac
    48601 catgacgcaa taacacaagt tttaacataa aaatatctct aaattattag ctaagaatat
    48661 ttatttttta tattataaca aataaaaaag agaaaaagca aattgactga gcagggcgaa
    48721 gatgatagta taaaaaagct taagtgtcca ataatattgc tcttaaaaaa aacctaaaac
    48781 ttaaaaaaat cgcggttgaa tcactgcttt ttggtctcaa gctgattatt ggtttgaaaa
    48841 acctgattgc cgtgacggta acgactgtta tcaagccgtt tttttggtta ggaaaatttc
    48901 tgctctatcg cccctttatc cgactgtact atctcatctt ccgtatcaaa aaatatgatt
    48961 taaaacaaca atcaaagcgg gaattaataa acaaaaaatc tctgcaccta accgctttga
    49021 ttttaattat ttccgctata atccttaatc taagcgataa aaaacagacc agcgcggcgg
    49081 tgggcaaggc gccgaaaagt ttgatggcgg aaattgtaaa aaatgaattc agcgacctcg
    49141 gtcaaaataa tgatgaacta gtagaagaga ctgcgcagaa tattaatatg cacgccttag
    49201 gtgcggaaaa atatctggga ggacgcgacg cggtcagtgt ggttaaaagt gttacccctg
    49261 taagcgaaga ggaaaataac cttgcaaaaa ataccaataa catgctgacc aaaccgaagc
    49321 aaatcgcaat tattaacaac cagacagaaa aaccggcaga aaataacaca acaacaagcc
    49381 gccaagaaat tacggaatat accgtccgtg ccggcgatac tatcagttca atcgcccgcc
    49441 gcttcaacct caatgtcaat actattttat gggcaaataa tcttaatgct ttcagtatta
    49501 tcagagctgg agacaagctc acaatcctac ctacagacgg cttcttatat aaagttgtcc
    49561 gtggcgatac tatcggtaaa attgcacaga cttatgatgt tagtgctgac aaaattattg
    49621 ccagcaactc tttagacgcc gccggcacga ttactattgg ccagcaactt atcattccgg
    49681 gagttaaaaa acccaccagc catacggtcg ccagcgccgg tactcaaagc agttactctg
    49741 gtattgctgt tattaaagat attatttcct ctgacaaacc gattgttagt agtaataaaa
    49801 tggtttggcc tacggttggc aatcgcatta ctcaatattt ttcctggaaa cacagcggtt
    49861 tagatatagc caataaagta gggacgccga tctatgcggc cgatgacggc gtcgtagaga
    49921 tatctcaagg aggctacaac ggcggttatg gcaatactat cctcataaac cacggtggcg
    49981 gtaaaaaaac cagatacgga catgtctcta aattattagt taaaaaagga gaaacagtaa
    50041 ctaaaggtca attaattgct ttaatgggtt cgaccgggcg ctcgaccggt cctcatctcc
    50101 actttgaagt cattatcaat ggtgtcagat ataatccatt aaactatata aaataatatg
    50161 attattctcg gcttaataat aatagcggta ggaacagtgc taattataaa aactgaatgg
    50221 tttctgcaaa attttggaag attatcttgg tttgaaaaca agcttggcag cgaaggcggc
    50281 tctcgtttgg gctataaact tctaggcctt gttgctatct ttattggaat agttctgatg
    50341 agcggcaatg gcaacgattt cttcacctgg ctactcggcc cacttattcc taaacaataa
    50401 tacataatat tgactagtca taataatctc ggtaaactaa aatttaccga gaaaaagggc
    50461 ccttagctca gctggtagag cacctgcttt gcaagcaggg ggtggcaggt tcgagtcctg
    50521 tagggtccac taatactcaa catctcctac cagtaattta aaagccatcc ttttatggat
    50581 g
//
LOCUS       DAOQSA010000003        40576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_478_length_40576_cov_9.186743, whole genome shotgun sequence.
ACCESSION   DAOQSA010000003 DAOQSA010000000
VERSION     DAOQSA010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 40576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40576
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_478_length_40576_cov_9.186743"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..291)
                     /locus_tag="PKK65_00665"
     CDS             complement(130..291)
                     /locus_tag="PKK65_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81234.1"
                     /translation="MYDFCFFKKIVVRQEIGRANDIKEIIEEELQSTLFDFSDFEWAK
                     EYIFDVVVR"
     gene            complement(261..983)
                     /locus_tag="PKK65_00670"
     CDS             complement(261..983)
                     /locus_tag="PKK65_00670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNU81235.1"
                     /translation="MKNKEFFYLQYNKVNWQNQEKTKINSFVDNFIIQNIISKKEGDE
                     IKIFDIGFGIGFFLRMLQKNLSKSFKTIILEGCEPSRKNYKYFKSKPLNLKNTRLRVF
                     NKTFLQTNTDTKFDFLTAIYVFTHFASDELNDVAQKINSMLNNEGKLILVVANEKYLE
                     EKLKSKKDLFIERNIFEFDNQKYKEVLHYSEIPQIGTIIDYNREEKFYPDLFKRHGFN
                     LDSKKDLDDNGFICTIFVFSKR"
     gene            complement(1242..1892)
                     /locus_tag="PKK65_00675"
     CDS             complement(1242..1892)
                     /locus_tag="PKK65_00675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNU81236.1"
                     /translation="MVTQYDKISQKFKNADESVPDVTRPILYSLVTKKKGKLLDLGCG
                     YGEDLVHFQKEDFEVYGIDVSKEMVKEAEKKSQGAKVSVSSFEKLPYDEETFDIIFSR
                     YALQHSDKITPIFKEVSRVLKKGGEFVFLVTHPIRHYFEKKTKDYWKQEICSSLILGG
                     KVRVEEPSHKLNEYINKEILSKFDLIDFIEEFDPAAEKIKDAGKYPSILILKFKKR"
     gene            2588..2776
                     /locus_tag="PKK65_00680"
     CDS             2588..2776
                     /locus_tag="PKK65_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNU81237.1"
                     /translation="MKTFLETREDDDQYLFTHLLKKGVDIRYIQKLLGHKGLETTQIY
                     LQVSTPKLQEINSPLDDL"
     gene            3036..3108
                     /locus_tag="PKK65_00685"
     tRNA            3036..3108
                     /locus_tag="PKK65_00685"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3069..3071,aa:Lys,seq:ttt)
     gene            3282..3980
                     /locus_tag="PKK65_00690"
     CDS             3282..3980
                     /locus_tag="PKK65_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81238.1"
                     /translation="MANNNQKLNMAEAIIGLCLANHNLAGAINAAILIDQRQLKYEEW
                     DTAYQNMGAKKIVATLLALNNQEANVRFIAYLKAGENIAELIDSATRLSIPISIGALK
                     IMVQRLIKQKKPALEIQIMAATRYVNLSSELLTTWLYLAKRMAVKNGNVTIALEASSR
                     LDKKFKLSVEEVEELLLSRIKKMTEESEKEMTEKEGHVLKECKQCLQLISLLPPEKQF
                     YKKLIFGDNADNAK"
     gene            complement(4092..4961)
                     /locus_tag="PKK65_00695"
     CDS             complement(4092..4961)
                     /locus_tag="PKK65_00695"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNU81239.1"
                     /translation="MRIDLQLHSLYSDGYHSPSQLVRMIYLQGIKAASLTDHNTIAGQ
                     AEFKRACARYRIKYIPGLELYVRYKQRTFNVLWYNYDLNSPVLLKMLDTTCLRRRRFA
                     EKVAGRWRRLGLKFNWRNFLREHPYYLSTNHLVNAVWKSPDNRRKIKEALELKNVREE
                     DIMRYCLFPKTGPRFQDAYVSLTKILQVRKETGGQLIFCHPGLNNKMKNGLVEKTIDA
                     GLDGFELLSPHHGYNAIMYLSSLFKRKKVIMTGGSDFHKPGDFGTKPRYSWDWFVVDS
                     DKLPGVNRILDNK"
     gene            5040..5116
                     /locus_tag="PKK65_00700"
     tRNA            5040..5116
                     /locus_tag="PKK65_00700"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5074..5076,aa:Thr,seq:tgt)
     gene            complement(5166..5390)
                     /locus_tag="PKK65_00705"
     CDS             complement(5166..5390)
                     /locus_tag="PKK65_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81240.1"
                     /translation="MAQEAGRFIPFERALLKQYQDTGSSVVKNFFDYLIEKRLLHLSG
                     LGWFLSNDKIVAPSDPACPNCPYKFKCFFG"
     gene            5535..6917
                     /gene="dnaB"
                     /locus_tag="PKK65_00710"
     CDS             5535..6917
                     /gene="dnaB"
                     /locus_tag="PKK65_00710"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNU81241.1"
                     /translation="MAGEDILLEKIPPHSLEAEESLLGCLLIDKDAIIKVADMILPQD
                     FYRQANQKIYEAIQELYNSQEPIDIITLANKLEDKKQLTDIGGRTYLAKLSNAVATAG
                     NVSQYASIIQKKATLRRLQQAAAEITNASFDEDREIDEILDETEKKIFSVSRKFLKNA
                     FLPLDSLLADAFERIDELHKHGGKLRGLSTGFNDLDSLLAGLQKSDLIILAARPSVGK
                     TSLALDIARQAAIKNKVGVGIFSLEMGKEQLVDRMICAQANVNLWKMRTGNLSDKEED
                     NDFTKIGKSMGELAEAPIYIDDSSSLSVMEIRAKCRRLQIEKGLGLIVIDYLQLMEGR
                     GKYSDNRVQEIGEISRGLKGIARELNIPVLALAQLSRAVEQSSPAIPKLSHLRDSGSI
                     EQDADVVMFIYRKAADRNYNPNDLSIDERYKAEIHIAKHRNGPTGKIDLFFDENTASF
                     RNLSRQNNTP"
     gene            6945..7220
                     /locus_tag="PKK65_00715"
     CDS             6945..7220
                     /locus_tag="PKK65_00715"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNU81242.1"
                     /translation="MFNKLKQFKDLRSQAKTMQSALAQESVTEEKNGVKLTLNGNMEI
                     IELTLNPELNTENQANAVKSCFNDAIKRTQRLMAKKLQDMGGLPGLN"
     gene            7221..7811
                     /gene="recR"
                     /locus_tag="PKK65_00720"
     CDS             7221..7811
                     /gene="recR"
                     /locus_tag="PKK65_00720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNU81243.1"
                     /translation="MKYPEYIQKLIESFSKFPSVGPKTAERYVFYLLNQKTVQVKELA
                     SFISSLPDNIKRCSICRTFSDSDPCPICGDKKRSPEILCIVENSQDLAVIENTKQFNG
                     RYFVIDRLLNTLENIGPDQLPLKELKELINANKVKELILGLNFTLEGESTSLFLKKTF
                     PEIKISRLARGLPAGSDLEYADELTLANALKYRNTL"
     gene            complement(7918..8232)
                     /gene="rplU"
                     /locus_tag="PKK65_00725"
     CDS             complement(7918..8232)
                     /gene="rplU"
                     /locus_tag="PKK65_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNU81244.1"
                     /translation="MAKIAVIATGGKQYKVKEGETIKVEKLELAAGEKVKFDTLLVAE
                     IDGSQLEIGSPSLGEKVEAEVIDTTKQDKVKVVKYKSKTRYKKNVGHRQLKTTVKILS
                     IA"
     gene            8363..9628
                     /gene="murA"
                     /locus_tag="PKK65_00730"
     CDS             8363..9628
                     /gene="murA"
                     /locus_tag="PKK65_00730"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNU81245.1"
                     /translation="MPKFIINGKKPLSGQIAVSGAKNSALKIIPASILSTEKITIKNL
                     PNIEDVTRSFEVLTDLGAKITYNGSEANIDTSNIANNELNAKMADKFRGSIMFVGPLL
                     ARFGEAKFPHPGGCVIGAGGRPIDLFLEGYRAFGAEIKEESGKFYHIKAKHLQAAEYF
                     FPLVSVTATESMMMTASIIPGTTILKNCAMEPEISALADYLNTQGAQISGAGTPIMVI
                     KGQNKIGAGEFTVIPDRIETGSFAILAAAANANLEIINCQPEHIMTLLKIMERIGVDF
                     EYGRDWLKIKKGKRPLKAHDIKTHEYPGFATDLQSPYVVLMTQAQGISLIHETIYDRR
                     LLWTDMLSQMGASIIMCDPHRVVIQGPTPLFGKRLISPDLRAGIALVIAGLIAEGRTE
                     IDNVYQIDRGYEKLEERLRKIGADISRVE"
     gene            9634..10968
                     /locus_tag="PKK65_00735"
     CDS             9634..10968
                     /locus_tag="PKK65_00735"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNU81246.1"
                     /translation="MPKKIRDWLFILFIILFVIITFFVSVYAAGYSLNKRWPPRFDQI
                     FQKTGMLIVDSEPSGASIFLNGERRRKSILLDIGRSDALTPQKIKNLPPGEYILMLEK
                     DGYWPLEKKINIYPGQTTFAEDLILFKRSLPLNLSLCAPQTLELSPDNRRLILPEEGT
                     AINLKTETKSDLGLQLSQGDIQWSKDGARILFGGKILNLDNGQSNDTLAMIGQEANNF
                     YWDENGKKIYYQADGHINCILTDNNTISTVLSGSDYVAYTVRGNLIYTIERQNEQMYL
                     RLYRADNHLLQNSINLPAGDYIFKQDKYHLNLYDRKQKALYLLNDYAQQPINKQLRPV
                     TAWQWLNASTLMWYNEFEIYSFDLNSNRQELLIRVSEKLTGAVWNRSKNYIIYANNDE
                     IKIINLNLDKRLPITLLKAEEISNLALDEKNQLLYFYAKIGQQAGIYKLQLQ"
     gene            complement(11066..11944)
                     /locus_tag="PKK65_00740"
     CDS             complement(11066..11944)
                     /locus_tag="PKK65_00740"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HNU81247.1"
                     /translation="MQKIKKAILPVAGFGTRFLPVTKAQPKEMLPVVDKPVIQYLVEE
                     AAAAGIEEIIFVTGRGKRAIEDHFDYSFELSKTLVEKNKLDLVKKVQAIENLAKFSYV
                     RQPIPLGDGHAVMCASHLVRDDEPVLVMFGDTLYDAPISPVKQVMDVYERYGDAVIGL
                     SEVEHKDVSQFGVIDGLDLGEGIYEIKKFIEKPSLEEAPSNLVQVGINIITPEIINIL
                     KSMQSGKSGEIRLADAYDVMLEQGHVLYGKQLEGEWLDTGNKFNLIKANIKIGIKDPE
                     IGEDLKDFLRGLAKSL"
     gene            complement(11968..12756)
                     /locus_tag="PKK65_00745"
     CDS             complement(11968..12756)
                     /locus_tag="PKK65_00745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNU81248.1"
                     /translation="MDLSIIIVNYKSRLKLEACLRSLATADLSDLEYEIILVENASGD
                     KLADLTAVDARIRLIESEQNLGMGGGNNLGIAASSGEMILIANPDLVFSREAVKKLVL
                     YLTNNREVAIVGPKLLNSDGTLQYSCARFPSIFLPVLRRTAVGHYLPGLIDRYLMKHE
                     NHQETRNVDWLLGACLLVRRGELFNHEKLFDERFFMYFEDVDLCRRAHEQGKKVVYYP
                     EATVIHDHQRKSARLPWYLAIFKDHLAQEHLKSWWKYFKKWGWR"
     gene            complement(12758..13816)
                     /locus_tag="PKK65_00750"
     CDS             complement(12758..13816)
                     /locus_tag="PKK65_00750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNU81249.1"
                     /translation="MKVGIILVNYKDYAQRFLTPCLASIRALDASCRPDIFIVDNAST
                     AESAAYLRDQAPAARFIFNKNNDGFAKGNNDALRLMMAENYDYALLLNMDASLAPRAL
                     TELVRIVEANPAAAAVQARLMFNSDREMINSLGNVTHFLGFGYCDAYRQTFLDRGEEV
                     KKIAYPSGAAVLLRMEALRAVGLLDEEFWMYNEDQDLGWRFWLSGWQCLLAFKAVAYH
                     QYEFSRSVSKYYWMERNRLIAAWKNYSILSLILFLPPLLVMELGMSFFALRSGWLKEK
                     KRAWAYFFNLKNWSYLLQARKQAQTLRCVPDYKILPLFSGHIWYQEIDNPVLKYLVNP
                     LFSFYFFIARRIIRICRQ"
     gene            complement(13820..14671)
                     /locus_tag="PKK65_00755"
     CDS             complement(13820..14671)
                     /locus_tag="PKK65_00755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNU81250.1"
                     /translation="MSLDVSVIIVSWKVKDRLRANLSALLPSLNGLSAEVFVVDNNSR
                     DGSAEMVSKEFSTVRLIANQENLGFAKANNQAIKEAKGDFILLLNPDMLVFPDTVKNM
                     LAWMKTNQQAVVAGCRLENESGERLPSVRRFPRLSDQLAIVLKFPHLFPSWLNSYLCK
                     NFDYSQAARVDSVRGAFFMINRAAWQNISASALPLLDERYFIWFEEVDFCRQVYKNGG
                     EVWYTPVARCLDYVGQSFNQVDVSNKQLYFQDSMLKYFQKWEPSWQCFVLKSAWPLGR
                     FLAKIFS"
     gene            complement(14675..14947)
                     /locus_tag="PKK65_00760"
     CDS             complement(14675..14947)
                     /locus_tag="PKK65_00760"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNU81251.1"
                     /translation="MDIADLQAKLNEKGKISFPVKVSPGADKTEWQEPLADGTLRLKV
                     KAAPERGQANAELVAFLAGIFRVNKKLVEITAGGGARLKRIKVKKS"
     gene            complement(14951..16189)
                     /locus_tag="PKK65_00765"
     CDS             complement(14951..16189)
                     /locus_tag="PKK65_00765"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNU81252.1"
                     /translation="MRYWRYLLIIIFIFLFLPFYSGQAASLADKLAGRIVLSVEEKGE
                     AWYINPLDLRRYYLGRPADAFQVMRNLGLGINESQFQEIAQAGMPVEGNLDLAKRLAG
                     RIILQVEKNGEAWYINPLDLKKYYLGRPADAFQVMRNLGLGITRQNLAQIHKPGLSES
                     INQYSGYEHKLVSTERGDFKVDVLRIDLKNPNLEILSLAADSQVCTDGVCRAQSLGDY
                     VFANQGFAGVNGSYFCSSSGCGRPNYYFFPVYDSLSGEMVNADQLKYWTTGPLIAFDT
                     NNKFYYFKDSREFKSVDDFEKNYGVKLQAALGNQPRLVENKMNVLIDWDLDKGQKTGK
                     YWRNALGYKDDPVNPGKGELYIVIAHQATIDDLAVIMKAMGMDYALNLDGGSSSALIY
                     NGEYMVGPGRDIPNALVFKQ"
     gene            16362..16859
                     /locus_tag="PKK65_00770"
     CDS             16362..16859
                     /locus_tag="PKK65_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HNU81253.1"
                     /translation="MSAEEKYIRPSWDEYFMKITEMVGSRGSCDRGRAGCVITRDRRI
                     VATGYVGSPIGLPHCDEVGHEMHTVTHEDGSTSRHCIRTTHAEQNAICEAARMGISIE
                     GGTLYCKMTPCYTCAKMIINAGIKKVVCAQDYHAGARSKEIFEQAGIEFTLLSEEVIT
                     YQDMK"
     gene            16865..17680
                     /locus_tag="PKK65_00775"
     CDS             16865..17680
                     /locus_tag="PKK65_00775"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNU81254.1"
                     /translation="MKVYLDLLKDVMENGKNKDDRTGTGTTSVFGRQIRFDLNAGFPL
                     LTTKKVHLKSIIYELLWFLKGDTNIKYLNEHGVTIWDEWADENGDLGPIYGKQWTAWE
                     APNGDKINQIAEAVEMIKNNPSSRRIIVSGWNVGDLRMLISGKKTAPPPCHSLFQFYV
                     DADDHLSCQLYQRSADLFLGVPFNIASYALLTMMMAQVTGKKLGDFVHTFGDLHIYNN
                     HREQVAEQLGRTPRPLPTMIINPEVKDIFSFQYEDFQLEGYNPLPGIKAPIAI"
     gene            17685..18191
                     /locus_tag="PKK65_00780"
     CDS             17685..18191
                     /locus_tag="PKK65_00780"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNU81255.1"
                     /translation="MIPVTLIMAVTVDGKIAKDKSQLANWTSQEDKKLFVEVSRDFGV
                     IMMGENTFKTFPKPLPGRLNVVFSPDGGENIEGLLKWVKGEPEIILAELEQLGYTKAL
                     LGGGSFLNTLFLKKKLINEIILTVEPKIFGHGLSLFTEICETNLNLLDCQKLNENTLM
                     LKYKVIYS"
     gene            18197..18751
                     /locus_tag="PKK65_00785"
     CDS             18197..18751
                     /locus_tag="PKK65_00785"
                     /inference="COORDINATES: protein motif:HMM:NF024635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNU81256.1"
                     /translation="MPKLVLGFVGKLASGKAVCQKYISEKYNAGSARFSTALRDILNR
                     LYLPISRENMQNLSFDLRKRFGSDILARVIAEDVKNDPHNIVVIDGVRRLADISRLTE
                     LSNFHLISINASPEIRYERMKLRNENAGDADKTFEEFLEDDRREAELQIPEVMAAASF
                     NLDNNGTLEELYSQIDKILEEIQN"
     gene            18831..20147
                     /gene="dut"
                     /locus_tag="PKK65_00790"
     CDS             18831..20147
                     /gene="dut"
                     /locus_tag="PKK65_00790"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNU81257.1"
                     /translation="MSNYSGKFIVIDGTDGTGKTTQLQILANRLHHEGYEVELADFPQ
                     YNTKSAGMVEEYLSGKYGEANDVNPYAASLFYSVDRFDASFKIREWLEQGKVVICNRY
                     ISASFAHQGNKIKNALERKLFYNWLSEIEYKVFNIPKPDLYLILYMEPEISQKLAHNR
                     RREDWKGKTKDIHEDNLQHLRQAAQTYLEIAQEMPDFRLIRCTRKQEILKREDIHYLI
                     WLYVNRILNSDQTTPKTPDLQALNDILINKSKLTPNLPELIDVPRLSISAAASVLGES
                     PTLGLPAHSDKDESPAIADKGINRLMVERLLPEAKLPERAHPYDAGLDLFAAEDCSIP
                     PYGQDLIPTGIRLAIPDGYVGLIWDKSGLASQGFTTMGGVIDSRYRGEIKVIFKNLSE
                     DIYHIQAGQKIAQLLIQKIELPDVQAGKIEDDSQRGGGGFGSTGLF"
     gene            20227..21735
                     /gene="gatB"
                     /locus_tag="PKK65_00795"
     CDS             20227..21735
                     /gene="gatB"
                     /locus_tag="PKK65_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNU81258.1"
                     /translation="MKYDVIIGMEIHAELKTKSKMFCSCSNDSDGKAPNTTVCPICLG
                     HPGTLPVPNKQAIDWTLLIGLALNCQINYHSKFDRKNYFYPDLPKGYQISQYDQPFCY
                     QGRLDIGNKDIAITRIHLEEDTGKLFHPQNSKDTLIDFNRAGTPLVELVTAPVIKNAE
                     EAKNFCQAYQRVLRFLEVSNADMEKGEMRCEANVSLQKPGTWVYENGLIVATGKKKLN
                     AKVEIKNINSFRAVEKAINYEITRQSEMLDNKEEIIPETRGWDEKKNATVAQRKKETA
                     ADYRYFPEPDIPPIIIDEAWLNQLKQNLVELPDAKQKRFQRQYGLKTESAAIIANDKE
                     LASFFENVVSEMGAWIEANGDDFERQDKNAAQMVANWINSELLKNLNTDKRAISDLKM
                     TAENFAELMSLIWQNKINSNAGQRILKIIYWEGGDPSDIMTKLGLEQLDSEEELTTAI
                     KKIINSNPRQAAEYKKGKKPLIQFFIGKVMAETKGKANPEKVKDLLEKILNE"
     gene            complement(21775..22725)
                     /gene="miaA"
                     /locus_tag="PKK65_00800"
     CDS             complement(21775..22725)
                     /gene="miaA"
                     /locus_tag="PKK65_00800"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNU81259.1"
                     /translation="MSPKAINLNKPKVLVLLGPTAVGKTALGVRLAWKYQGEIVSADS
                     RQVYQGMDIGTGKDLAEYKVGRKHIPYHLIDVISPNTEFNLAQYQKLALEAISDIINR
                     GQLPIIVGGTGLYLQALVDNYDLSKISPDLEKRRELEALTKDELFGRLKKLKPEFALK
                     LNNSDKNNPRRLVRYLEIVEKDGDLSNKQDSPYDFLVLGLDFPDDILRERINKRIKNR
                     LEKEDMLSEVERLHNSGVSWERLISFGLEYKFLSYYLLGRLDYDEMVQKLGDASYRFA
                     KRQKTWFKRWERQGRKIHWLRGQSEAEVLSEAEVLIDNYL"
     gene            complement(22805..24106)
                     /gene="miaB"
                     /locus_tag="PKK65_00805"
     CDS             complement(22805..24106)
                     /gene="miaB"
                     /locus_tag="PKK65_00805"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNU81260.1"
                     /translation="MQYHLITIGCQMNKADSERIASFLEKRGYVASSSWLGVDLVVLT
                     TCGVRQSAEDRVYGLVNRIRQGNPKAAVVITGCLARREDVKRRLRGKVDLFMPINELP
                     NIFALLKDSHRQLSLSTDAVRELSGEKYLTIIPKYQSTFTAYIPIGNGCNNFCSYCVV
                     PYARGREVYRPAADVIKEAKALLKRGYKEIILIAQNVNSYRSGRTNFLQLLSKLAALP
                     GNFWLRFSSSHPKDLSPELIKVLASSDKICHHLHVAVQSGDDEILKAMNRHYTAAHFS
                     SLIKKIRKAKPGIALTTDVIVGFPGETKKQFQNSVKLFKNLQFDMAYIAQYSPRPGTA
                     SWYMKDNVSREEKHRREQILTEILRKSGKKANRAYWRRTVIVLVEGKNRRGGYYGKTS
                     SFKTVSFTAPSEVRPEDIIGNFVSVKIQKVQDFGLEGVLKK"
     gene            complement(24120..25910)
                     /locus_tag="PKK65_00810"
     CDS             complement(24120..25910)
                     /locus_tag="PKK65_00810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNU81261.1"
                     /translation="MIKFRLFKNNFDYFLVFLLAFVFLVATAWFNYATQSSDYVKFLS
                     PDETANYFFAKHYAETGSIAVFEPANLIAEEIVHPRSIRSDNGWLKPVSFLGIILIYG
                     QIASWLGTAIIPFLTPLFAAFGIIFFYAFVRRLFGRQVALISAFLLASFPVYFFYSAR
                     SMFHNVLFLVFLLAGAYFLLLSLPSRTSDKRKFWTWHLSAQDCLTYIFSFLTGLMFGG
                     AIGTRSSELLWLGPVLFIAWLFYARRLGLTRLVLMLGGLVLALLPIFYWNQILYASPF
                     FGGYGEMNRSLTEISQVGGQFLQSSVRGDFSHYRLAAATLIDNVFYFGYQPYQSLKMF
                     FYYVLKMFPALSAFAFAGFLIFIYRIIRRPNRGAYLYLITWLVLSLILVFYYGSWHFN
                     DNPDPKRFTIGNSYTRYWLPMYIMAIPLVSLFVVSVSRFITSISWPRFDFSLKRRWRR
                     FLAIGLSALITCFLIFVNLEFVFFGSEEGLATLYHNYFVDKTNAQAIIGLTEERAIIV
                     TQYHDKQLFPQRKVVVSLLTDADVNKSLGKILRFYPIYYYNFTFPNKDLSYLNERRLP
                     QFGFIINPVVHRGQFTLYRLDKIEPLVESK"
     gene            25989..27905
                     /locus_tag="PKK65_00815"
     CDS             25989..27905
                     /locus_tag="PKK65_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81262.1"
                     /translation="MLKFKFSLTFDSLWLWLMLFTSSVALVYFLHALDIIGLIAAFLI
                     ATIIYAVWKKQIKEQISTKVSIKNSKYFWFFSLMTLTMIIIMVIMAAGNATDKAIISP
                     WTVINPWFFVVLFAANLSLIGALSQTAKSLFKNILLGLYYLEIFSVAAIVYRLGYGFD
                     PFIHQAAMAEINRIGFILPKTPYYIGQYGLIIALYKISGLSLALINQYFLPLVSALSL
                     PWILGQLRQKSSRPEAWLAIILLTLLGFSPFILSTPQNFSYLLLLATIIFIYRQKNEA
                     LIISTALATLAVHPLAGIPAILAAGWFIIQKRWPRLKISRFFKNPIIIFGVALLAFCF
                     SLWAVAGFGSLSWQNFNLSLLNPVFKNSENYWLNLGYFFINNYFWLILGMVVIIIFKR
                     RQIWPEEQLKTENNIRLLSSASLAAITAYLISRGFTFNNLIAYEQENYASRLPIIALI
                     IALPLFYELFRYLSQQALKMEKSFKIIAAIGLALMMTVSVYASYPRFDNYYNSRGYST
                     SAADIKSVQRAEERAQGEKYIALANQQVSAAALHEFGFHHRYIKKEGQEIYFYPIPTG
                     GPLYQYYLNMVYKKANRQNMLAAMDFADVNRAYLIINKYWWASDKIIAEAKMSADYWE
                     KIEQGENYLFEYRR"
     gene            complement(28024..30387)
                     /locus_tag="PKK65_00820"
     CDS             complement(28024..30387)
                     /locus_tag="PKK65_00820"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HNU81263.1"
                     /translation="MFEEVKIPSSGANNFIEAANQGAGEDKSGAKKKILILILAIIII
                     AALGAGIWWLSQRLKSSSQPSGDDSQASSSAPARLNDFHLEGIDGQKEGSNTAEEPNA
                     VEYLYFSYFYHEPDNIIDDFKFPEYAVPINVKLDVSNYYDVSRKLNLDSSLESLNKNG
                     FAILDNPWPKEANDFYALGALLDDKQIPLFISADFISYYYQSILKMSFKEIEEGVFYE
                     SLWNINRTLYEGARIRYENHLQVSGNINDRVLEGERLEAAFFAVSLELLKPRLDQVDS
                     ANNLNSGKFSGRDQQKFSFTLPAYLSDDVTKELELIRAAKEQQKSPVLLYDRDYKSFI
                     IPQEYKNNARLQNFYLTAVWLNSVFPLNYRDESCPECLLDKDDWRINFTAASLIASDF
                     ANNQELKSEWARIYKIISFFKGLRDGWNYVHYRDGLKNSFGDDFDVTRLFAEDNPEAE
                     ANMEKLRQNLLQREVVPMQGGLSLKTMAGYKIAGLQFLADFYWPNDFIFGHLRYPEVG
                     VYQGGEKPVANNVTACLVQKKYQRCQGSSQDILSLLYPAWNGPLFLENGNYANYKEAL
                     ENLRPLVEGGMNENLNNYWSSLLANRYYLNISDTRLPAYLRSKDWRAHMANSVLGSWT
                     DMQLPLDKISLRSQSTNNSNLGATVSFSEYNWVEPNIDYLDRLLAHNQMILGMFEAIG
                     LDEKSSLATNNIREAGRQLAGLRAIIAKQAVGEDLNADDNRFIRDFVRAYLVEEPAEK
                     TLSWRNNSLGANIKESLGSPRLLIIAHPVSDKIVFSVGPVFNHQESK"
     gene            complement(30392..31321)
                     /gene="mltG"
                     /locus_tag="PKK65_00825"
     CDS             complement(30392..31321)
                     /gene="mltG"
                     /locus_tag="PKK65_00825"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNU81264.1"
                     /translation="MRQIVRYFILLVLSLLVTTIVLSLVFSRSLLSSLGPVNGLLARL
                     DNIFSSSEDKKIEPRPEKTVRLIEGWTSRDMGQYFESQGIWQSEEFLEFVGFPQIDYN
                     KEKDLPPLEDFSDDFSFLSDKPKNRGLEGYLYPDTYRIYATSSPRELVVKMLQNFDNQ
                     LTPKMRADIKKQGKTIYEIVTMASLLEKEAPINYKSGDNRDARIISGIFWNRIKYGQA
                     LQSCATLAYVLGVNKSQYSSADIQTESLYNTYKYRGLPPGPISNPGILAIEAAIYPLS
                     TNYNYFLTPAGSKDIIYSTTYSEHLRNKSKYLP"
     gene            complement(31332..31799)
                     /locus_tag="PKK65_00830"
     CDS             complement(31332..31799)
                     /locus_tag="PKK65_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HNU81265.1"
                     /translation="MYSHKKTTNIPKKPIVVAVSGYFNPLHVGHLEMIARAKKLGDKL
                     VAIINNDFQVKLKGSVPFMSLKDRVKIISALRDVDEVFVSIDRDKTVCKSLAKLKPDI
                     FANGGDRHSLGDVPEYPICQKYKIKMVDGLGKKIRASSVLIAKAAAKKAKIKS"
     gene            complement(31938..33599)
                     /gene="groL"
                     /locus_tag="PKK65_00835"
     CDS             complement(31938..33599)
                     /gene="groL"
                     /locus_tag="PKK65_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255396.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNU81266.1"
                     /translation="MAKQIIFDEKARLALKRGADQLANAVKITLGPKGRNVVIDRSYG
                     SPIVTKDGVTVAKEIELEDKFENIGASIVKEASAKTSDAAGDGTTTATVLAQAIITNG
                     LKLVAAGVNPIEIRKGIEVRVAQIVSALKQMSKSISTKEEIAQVGAISANDEEIGKII
                     AEAMDSVGKEGVITVEEGQSFGIEKEVVEGMQFDKGYVSPYMISNQETMKAEMSDPYI
                     LVTDKKIASLQEILPLLEKIAQSGKKDLVIIAEEIEGEALATFVVNKLRGTFNVLGIK
                     APGFGDRRKAMLEDIAVLTGARLISEEVGLKLDKAEIEDLGRARRVVASKDNTTIIDG
                     AGDKKAIEDRIAQIKKEKELSDSDFDKEKLQERLAKLSGGVAVIKVGAATETEMKEKK
                     YRIEDALNATKAAVEEGVVPGGGLALAVACYGLDFAGFEKEMKDGGSLPAGEKIINQS
                     VLEPIKQIANNAGVDGSLVLQRIIAENKKSKEVRGYNAATGEFVEMISAGIVDPTKVV
                     RSALENAASAAMMFLTTEAVITDKPEAHNSHNHGGGMSGMDMDMM"
     gene            complement(33628..33918)
                     /locus_tag="PKK65_00840"
     CDS             complement(33628..33918)
                     /locus_tag="PKK65_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNU81267.1"
                     /translation="MTLKPLSNHVIVKPAVQEEVTKSGIVLPDTADKERPEKGEVIAV
                     GPGKIRDNGQLSPMSLKVGNQVVFKKYSPDEIKIDGEDYLILSEDDVVAIIE"
     gene            34154..34933
                     /gene="uppP"
                     /locus_tag="PKK65_00845"
     CDS             34154..34933
                     /gene="uppP"
                     /locus_tag="PKK65_00845"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002866361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HNU81268.1"
                     /translation="MNILHSLILGIIEGITEFLPISSTAHLIITAKALKIPSSDFLDS
                     FLISIQLGAILSVAVLYAQTIIRQPRLILKITAAFIPTAIIGLASYKLAKTYLFESLP
                     LIAGALLVGGIILIVLEKYFAQHQRQNDEQSLARISYRQAAYIGIFQSLAIVPGVSRS
                     AATIMGGLALGVSRKNIVEFSFLLALPTMAAATALDLYKTPPLLQGNEAIIWLIGLAV
                     SFITAILGIKFFLKFIQKNNFQVFGWYRIILGIIIFAWILL"
     gene            complement(35117..35557)
                     /gene="atpC"
                     /locus_tag="PKK65_00850"
     CDS             complement(35117..35557)
                     /gene="atpC"
                     /locus_tag="PKK65_00850"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HNU81269.1"
                     /translation="MADRKIIKFEIVTPERTVFKQEVFQVTVPTQSGEITVLPDHIPL
                     VSILKPGVLEIKTVDQETEIISVSGGFIEVLRDKIVILADTAERAQELDENIIAEARR
                     RAEVSKEEAMSQDNYDLSAVAAKLEIEVARERALHKWRKLKNIK"
     gene            complement(35563..36954)
                     /gene="atpD"
                     /locus_tag="PKK65_00855"
     CDS             complement(35563..36954)
                     /gene="atpD"
                     /locus_tag="PKK65_00855"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNU81270.1"
                     /translation="MTEKNNNLGSVKQVIGVVVDVYFDKALPAIYNALEVDLNGKKLI
                     LEVEQHLGSNIVRTVAMGSTDGLKRGAEVLDTGMPISVPVGQETLGRIFNVLGEAVDG
                     GETPKTEKKYSIHRPAPKFTEQSSNVEILETGIKVIDLICPIVKGGKVGLFGGAGVGK
                     TVVILELIHNIASEHGGYSVFAGVGERTREGNDLYHEMKDSGVLDKVSMVFGQMNEPP
                     GARARVALSGLSIAEYFRDEKHQDVLFFVDNIFRFTQAGSEVSALLGRMPSAVGYQPT
                     LSTEMGELQERITSTKDGSITSIQAVYVPADDLTDPAPATTFGHLDSTVVLSRSLSEL
                     GIYPAVDPLDSSSIILDPKVIGQDHYEVARGVQKILQRYKDLQDIIAILGMEELSAED
                     KLLVARARKIQRFLSQPFTVAEAFTGRPGKYVKLSETVKGFKEILEGLHDDRSEDDFY
                     MKGGIEEVAAANA"
     gene            complement(36987..37931)
                     /gene="atpG"
                     /locus_tag="PKK65_00860"
     CDS             complement(36987..37931)
                     /gene="atpG"
                     /locus_tag="PKK65_00860"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNU81271.1"
                     /translation="MAKTKEISRRIKSISNTRKLTKAMEMVATAKMRRAVEAVLKTRT
                     YANLSWETVLHLSATNGQNGEKHPLLTLRPETKKAAVILITSNRGLCGGYNAAIINKV
                     KGSIKRHPEVPLDFILVGKKGAAVYAQNQTVTAEFPKSDILEDSKEVVPIVNLVIKDF
                     LGGRYDKVFLAYTDFVSPVKQVPRIKQLLPININTENEYLGVIGDPKVSVNKEMLSDK
                     ADKHLTPQAGFDYIFEPSPSEVLDNIIPRLLEVQVFQALLEANASEHSARMSAMHQAT
                     EAAGEMLNELTLFYNKARQAGITAEIAEISAGANALLN"
     gene            complement(37955..39478)
                     /gene="atpA"
                     /locus_tag="PKK65_00865"
     CDS             complement(37955..39478)
                     /gene="atpA"
                     /locus_tag="PKK65_00865"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNU81272.1"
                     /translation="MSSTKDFIVEQIKRQIADFQVSLEESSVGRVLKISDGIALVSGL
                     TEAMMSEILIFKSSQGEIAGLALNLEENLVGAIIMGDFSGIKEGDEVVCTKRILSVPV
                     GEVLVGRVVNPLGEAIDGKGVLATKEFYPVEKIAPGVITRAPVNQPVQTGIKAIDAMI
                     PIGRGQRELIIGDRQIGKTAIAIDTIINQKGQNMKCIYVAIGQKESKVAEIVRKLEAS
                     GAMEYTTIVLAGASSPASLLYLAPYAGTAMAEYFLDKGEDVLIVYDDLSKHAVSYREI
                     SLLLRRPPGREAFPGDVFYLHSRLLERACKLNKDFGGGSITALPIIETQAGDISAYIP
                     TNVISITDGQIFLEPDMFYKGNRPAINAGLSVSRVGSSAQIKAMKKVAGKMRLEAAQF
                     RELAAFAQFGSDLDEDTNRKLERGKRLYEIFKQDQYQPLSIDKQVVIYYALINGYIDK
                     VPVEKVRDFESGLYKYLEANNEVSKAILEKKELDVETEEKIKQLLGDYKETLDYLLS"
     gene            complement(39483..39887)
                     /gene="atpH"
                     /locus_tag="PKK65_00870"
     CDS             complement(39483..39887)
                     /gene="atpH"
                     /locus_tag="PKK65_00870"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNU81273.1"
                     /translation="MKVNAKQYARSLFELLEGKTENEAKSLIANFVKFLYRRQDFDKA
                     KAIINELESLYDSSDGLTRAELLSSRPLDNQIKSKISAYIKSKLGKTDIELSEKIDKD
                     LIGGFILRYNGLVIDGSFKNNLLKFKKQLLIN"
     gene            complement(39892..40386)
                     /gene="atpF"
                     /locus_tag="PKK65_00875"
     CDS             complement(39892..40386)
                     /gene="atpF"
                     /locus_tag="PKK65_00875"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNU81274.1"
                     /translation="MGLIKALGIDGRILLAQLFNFALLVFILWRYAYKPVLNILEERR
                     SKIEQGLNDAETAAEKLKQAEENSRQVMIAARQEAAQLLEKAQHQAELRQQEIVRKAQ
                     EDIGIMMEKEREKIEAEKKSVLAELKGELSNLILLGLKSFLHENVDDKRDKEAIDRLV
                     KDLI"
     gene            complement(40451..>40576)
                     /gene="atpE"
                     /locus_tag="PKK65_00880"
     CDS             complement(40451..>40576)
                     /gene="atpE"
                     /locus_tag="PKK65_00880"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNU81275.1"
                     /translation="EAIGRNPEANGKIAPLMFVAMAITESTGIYSLVIALIILFA"
BASE COUNT        13020 a         8921 c         7232 g        11403 t
ORIGIN      
        1 attcaattta taaaaatatc tattggcacc cgaaaatcaa ttttgtctaa cgcttacgaa
       61 tatgcaaagt ttttgcacaa tgaaatggaa caaaaatttg ggcgaaacta gaaatttaaa
      121 atttattttt tatctcacaa caacatcaaa aatgtattct tttgcccatt caaaatcaga
      181 aaaatcaaac aaagttgatt gtaattcttc ctcaattatt tcttttatat cgttggctct
      241 tcctatttct tggcgcacga ctatcttttt gaaaaaacaa aaatcgtaca tataaatcca
      301 ttatcgtcta aatctttctt tgagtctaaa ttaaaaccat gcctcttgaa taaatcagga
      361 taaaactttt cttctctatt ataatctatg atagtgccaa tctgtggaat ctctgaataa
      421 tgaagaactt ctttatattt ctggttgtca aattcaaata tgtttctttc aatgaaaaga
      481 tcttttttgg attttagttt ttcttctaaa tacttttcat tagcaactac taaaataagt
      541 ttgccttcat tgttcagcat tgaatttatt ttttgggcga catcatttaa ctcatcggat
      601 gcaaaatggg taaaaacata tatcgccgtc agaaaatcaa attttgtatc tgtatttgtt
      661 tgaagaaaag ttttattgaa aactctaagt cttgtgttct ttagatttaa tggttttgat
      721 ttgaaatatt tataattctt gcgagaaggt tcgcaaccct caagaattat ggttttgaaa
      781 gatttggata aatttttttg caacatccga agaaaaaaac caataccaaa accaatatca
      841 aaaattttaa tttcatctcc ttctttcttt gaaatgatat tttggataat aaaattatct
      901 acaaaagaat tgatttttgt tttttcttga ttctgccaat ttactttatt atattgcaga
      961 taaaaaaatt ctttgttctt cataataata tttaaatgaa tttattaaat ttactcttta
     1021 cgcgcgcttt tctaagccga tccctgattg aatataacat tgcgattaac agaagttctg
     1081 cgaagcagaa ttcaggaaaa tttcgcagga atttccctgt taatcgctgt taggcgtcct
     1141 gaacgaagtg aaagcccgca gggcgtggca aaaattctgc gaatttttga gttcagaagg
     1201 tctcgcggta gtgggacggc gcgcgttgtt ttaacataaa attatctttt tttaaatttc
     1261 aaaattagaa tacttggata ttttcctgca tctttgattt tttctgcggc agggtcaaat
     1321 tcttcaataa aatcaattaa atcaaatttg gaaagaattt ctttgttgat atattcattt
     1381 aatttatgag aaggttcttc aactcttact tttccgccta aaattaacga agaacaaatt
     1441 tcttgtttcc agtaatcttt tgttttcttt tcaaaataat gtcttatggg atgagtaacc
     1501 aagaaaacaa actctccacc ttttttcaag acacgagata cttctttaaa aattggagta
     1561 attttgtctg aatgctgtaa agcatatcta ctaaagatta tgtcaaaagt ttcttcatca
     1621 taaggaagtt tttcaaaaga agaaacagaa acttttgcac cttggctttt cttttctgcc
     1681 tctttaacca tttcttttga aacatcaatt ccataaactt cgaaatcttc tttttgaaaa
     1741 tgaactaaat cttctccgta accacaaccc aaatccaaaa gttttccttt cttctttgtt
     1801 acaagagaat aaagaattgg tcttgtaaca tcaggaacag attcgtcggc atttttaaat
     1861 ttttgagaaa ttttatcata ttgagtaacc ataattttat ttaaattaga taatttatat
     1921 atgtttcttt ttagcgcgcc gtcattttgg gtgggtggtc ggagaaagag tgaccttcta
     1981 aattacgcct aacaagtttt aaccacacct atttaaagct tgcttaaaaa tcgacctttt
     2041 tgtgtaatca tatacactat gaacacagaa tttcgaacaa ccagaattga acgcgaccca
     2101 atgtataact ttgaacgcga gcttaagatt cgcggattta gtcgtaagac catcaagggt
     2161 tatctacatt ataacagaaa attcttaaat tatacaaaaa agtccaaaag aaattgccaa
     2221 tgaagatatt aaaagatatt tggctaataa agaagtttct aacgctactt tgagtttagc
     2281 catcaacgtc ttgaaatttt attatactta agttcttaaa agaagatttt tttaatatca
     2341 aacacccaaa gaaagaaaaa cgcctaccgg ttgttttaac taaaaatgaa attagaaaaa
     2401 tggttgaagc gacagcaaat ctaaagcata aattactaat gaaaattatg tatgtttcgg
     2461 gattaagagt ttcagaagcc attaaattaa ataaaggata ttgatttagc agaaggaatt
     2521 atcagggtca atttaggcaa aggaaaaaga tcgccaaact attctttcaa aaagagccaa
     2581 taaagatttg aaaacttttt tagaaacaag agaagatgac gatcaatatt tattcaccca
     2641 tttgttaaag aaaggagttg atattagata catccaaaag ttattaggcc acaaaggatt
     2701 agaaacaacc caaatttacc tccaagtttc cactccaaaa ttacaggaaa tcaatagtcc
     2761 tttagatgat ttataagaaa atgactaaaa gatttttgta aaattttttc ggagtgggga
     2821 gtgtttccac aattctgcgt tagcagaata tcaaaaacac tgggcgggat tcaaaaatcc
     2881 gcttgcagat ttttctcaaa atgagttcaa atttcggcca gcaaatacta cataataaaa
     2941 aacagccata ctaatacaaa cccttgtcaa aaaaaaatat ttttgttaac atcagaataa
     3001 ttacatctgt tgttaaattt tttatttaac aaagagcgcc tatagctcag ttggtagagc
     3061 agctcccttt taaggagacg gtcgtgggtt cgaatccccc tgggcgcaca cataaaaaga
     3121 cttggttgac taagtctttt tcttttgttg ttattattga cttttagaaa aaataattgt
     3181 aatttaaaca cttaaggctc tttaaaaata agattaaagt gatctccctg ttgacaaaaa
     3241 atttcacttg aacgcagaga aatcacttta atctttaaaa aatggcaaat aataatcaaa
     3301 aattaaacat ggccgaggct ataattggat tatgtctcgc caatcataat ttggctggcg
     3361 ccatcaacgc cgccattctt attgaccaac gtcaactaaa atatgaagaa tgggatactg
     3421 cttatcaaaa catgggggca aaaaaaattg ttgcaacttt attagctttg aataatcaag
     3481 aggcgaacgt acgttttatc gcttatttaa aagcggggga aaatattgct gaattaatag
     3541 attcagcaac ccgcttatcg ataccaataa gcataggcgc cctaaaaatt atggtacaac
     3601 ggctgatcaa acaaaaaaag cccgcgcttg aaattcaaat catggccgcc actagatacg
     3661 taaacctaag ctcagaattg ttaactacat ggctgtatct ggccaaaagg atggctgtaa
     3721 agaacggcaa cgtgacaatc gcccttgaag cctcgtcccg gcttgataaa aaatttaagt
     3781 tatccgtaga ggaagtggaa gaacttttgc tttctcgtat caaaaaaatg accgaggagt
     3841 cagaaaaaga aatgacagaa aaagaggggc acgtcctgaa agaatgtaag caatgcttac
     3901 aattaatcag cctgcttcct ccagaaaagc aattctacaa aaaactgatc ttcggtgata
     3961 atgcggataa tgcgaaataa tatttggcca cacacactca aaaagccacc ctttctcaag
     4021 gatggctttt ttcagacggg gttttttaat cttataaaaa tgaccactcc cacaaaggta
     4081 agcgattgtt attatttatt atccaaaatc ctattcaccc ccggcaactt atcgctgtcc
     4141 accacaaacc agtcccagga ataacgaggt ttagtgccga aatcgccagg cttgtgaaaa
     4201 tcggatccgc cggtcataat cacctttttt ctcttaaaca aactactcaa atacatgata
     4261 gcattataac catgatgggg ggatagcaat tcaaagccgt ctaaaccggc atcaattgtt
     4321 ttttcaacca aaccgttttt catcttatta ttcaatcccg gatgacaaaa aatcaactgt
     4381 ccgcccgttt cttttctaac ctgtaaaatt ttagtcaaac tgacatacgc atcctgaaaa
     4441 cgaggtccgg ttttaggaaa aaggcaatag cgcataatat cttcttcgcg aacatttttc
     4501 aactctaaag cctctttaat cttccggcga ttatcaggcg atttccaaac ggcattaact
     4561 aaatgattag tcgacaagta ataaggatgc tcacgcaaaa aattacgcca attaaatttt
     4621 aaaccgaggc gacgccagcg accggccacc ttttctgcaa accgacggcg gcgcaagcag
     4681 gtcgtatcca acattttgag taaaacggga gaattaaggt cataattgta ccataataca
     4741 ttgaaggtcc tctgtttata acggacatac agctccaagc cggggatata tttaatccga
     4801 tagcgggcac aggctcgctt gaattccgct tgtccggcga ttgtattatg atcggtcaaa
     4861 gatgccgctt ttataccctg caaatatatc atgcgcacca actgagaagg cgagtgataa
     4921 ccatccgaat ataaagaatg taattgtaag tctatcctca tattttaaat atacaatatt
     4981 cagggtaagt tcacaaatct tgccaaacaa cataaaatca gataacattg aaaaataatg
     5041 ccgccttagc tcagttggtc agagcagctg ttttgtaaac agcaggtcgt gggttcgaat
     5101 ccgacaggcg gctccaataa aaaagccgtt tcctataaac gaagaaacgg ctttttgttg
     5161 gcaattcagc caaaaaaaca cttaaactta taaggacaat tcggacaggc cggatcgcta
     5221 ggggccacaa ttttgtcgtt gcttaaaaac catcctagac cacttagatg gagtagtctt
     5281 ttctcaatta aataatcgaa aaaattcttg accaccgaac ttcctgtatc ttgatattgt
     5341 ttcagcaggg ctctttcaaa gggtataaag cgaccagctt cttgggccaa atactcttaa
     5401 atcaataatt tgtcaattcg tataacgtgc tatatacata acctgagtat aaagcgtgta
     5461 caaagagtga ataaaattta cttgataagc gcctgataat aagataagat ataaaaacaa
     5521 tgaactgtaa atatatggcc ggagaagaca ttttgttaga aaaaatacca ccccatagct
     5581 tagaagcgga agaatcgctt ttgggctgtc ttttaatcga taaagacgcg attatcaaag
     5641 tagcggatat gattcttccc caagacttct accgccaagc taaccaaaaa atctacgaag
     5701 ccatccagga gttatataac agccaagagc cgattgacat cattactcta gcgaataagt
     5761 tggaagataa aaagcagttg accgatattg gtggtcgcac ttatctggct aagctatcaa
     5821 acgcggtcgc aaccgcgggc aatgtcagcc aatacgccag catcatccaa aaaaaagcaa
     5881 ccttacgccg attacagcaa gcggcggcag aaataaccaa cgccagcttc gatgaagata
     5941 gggagataga tgaaatcttg gatgaaacag aaaaaaagat tttcagcgtt tctcgcaaat
     6001 ttttaaaaaa tgccttcctc cccttagaca gtctattagc cgatgccttt gaaagaatcg
     6061 acgaattaca taaacatggc ggcaaattaa gaggattaag taccggtttt aacgaccttg
     6121 attccctgct ggccggatta caaaaatcag atttgataat acttgccgct cgacccagtg
     6181 tcggtaaaac ttccctagct cttgatattg ctcgccaagc ggccattaag aataaagttg
     6241 gagttggcat cttctcttta gaaatgggta aagaacagct agttgaccgc atgatttgcg
     6301 cccaagcgaa tgtaaatctc tggaaaatgc gcaccggcaa tctctcagat aaagaggaag
     6361 ataatgattt cacaaaaatc ggcaaatcaa tgggagagtt agcagaagca cccatatata
     6421 ttgatgactc cagtagtttg tctgtgatgg aaatccgtgc caaatgtcga cgcttacaaa
     6481 tagaaaaagg cttgggtcta attgtcatcg actatttaca gttaatggag gggcgaggca
     6541 aatatagcga caaccgtgtt caagaaattg gagaaatcag ccgcggcctg aaaggtatcg
     6601 cccgcgaact aaatatccct gttttagcgc tagcccaatt atcgcgcgcg gtcgaacaaa
     6661 gtagtccggc tattcccaaa ctttcacatc tgcgcgactc cggttccatt gaacaagacg
     6721 ccgatgtcgt aatgtttatc tatcgcaaag ctgccgaccg caactataat cccaatgact
     6781 tatctataga cgaaagatat aaagcggaga tccatatcgc caagcaccgc aacggaccaa
     6841 ctggtaaaat cgacttattt tttgatgaaa atacggccag cttccgcaat ctcagccgcc
     6901 aaaacaatac accttaaaaa aattaaaaat aataatataa acctatgttc aacaaactca
     6961 aacaattcaa agatttgcgc agccaggcaa aaaccatgca aagcgccctc gcacaagaaa
     7021 gcgtcaccga agaaaaaaac ggcgttaaac taacattaaa cggcaatatg gaaattatag
     7081 agttaacttt aaatccggag ttaaataccg aaaaccaagc caacgccgtc aaatcatgct
     7141 ttaacgatgc aattaaaaga acccaacggc tgatggctaa aaaattacaa gacatgggcg
     7201 gcctccctgg cttaaactaa atgaaatacc ctgaatatat tcaaaaacta attgaaagct
     7261 tttctaaatt tccaagcgtc ggaccgaaaa ctgccgaacg ttatgttttt tatttactga
     7321 atcaaaaaac cgttcaagtc aaagaattag cttcctttat atcatcactg ccagataata
     7381 tcaaacgctg ttcaatctgc cgcactttta gtgacagcga cccttgtccg atttgtggtg
     7441 acaaaaaaag atctccagaa attctttgca tcgttgaaaa tagtcaagat ttagccgtaa
     7501 ttgaaaacac caagcagttt aatggccgtt attttgtaat cgaccgactt ttaaacactc
     7561 tggaaaatat cggccccgac cagctcccac ttaaggaact gaaagaatta attaatgcta
     7621 ataaagtaaa agaattgatt cttggtctca actttacttt agagggagaa agcacctctt
     7681 tatttttaaa aaaaacattc cccgagatta aaataagccg actggctaga ggcctgccag
     7741 ccggttcaga tttagaatat gccgatgaac taaccctggc caacgcttta aaatatagaa
     7801 acaccttata acttgtactt caaaaaaagt taaacataaa tttaaagcag aaacataaaa
     7861 aaatccgcct ctaataaaaa gcgggttttt atgtcttaaa attatttctt aaaaatatta
     7921 agcgatactc aaaatcttca cagttgtctt caactgacga tggccaacat ttttcttgta
     7981 acgagtttta cttttatact taactacttt tactttatct tgcttagtgg tgtcaataac
     8041 ttcagcctct actttctcgc ctaaagacgg gctgccgatt tctaattgac tgccatctat
     8101 ttccgccact aataaagtat caaatttaac cttttctccg gctgccaatt ctagtttttc
     8161 cactttaatg gtttcgcctt ccttgacttt atattgtttg ccgccggtgg caatcactgc
     8221 aatcttcgcc atattattta atattagcaa aattattaga aaaaatcagg ctctcgccga
     8281 ttcaccttta aatattatca aatatttgaa aaatgtcaat aaataagata agataaagtt
     8341 aatataaaaa tataaagcct ttatgcctaa atttatcatt aacggcaaaa aacccctaag
     8401 cgggcaaatt gcagtcagtg gcgctaaaaa ttcagcccta aaaatcatcc cagcatcaat
     8461 tctgtcgacc gaaaaaataa ctattaaaaa tttgccgaac attgaagatg tgacccgttc
     8521 gtttgaggtc ctaactgacc taggcgcgaa gattacgtat aatggctcgg aggctaatat
     8581 agacacctca aatattgcca acaacgaatt aaatgccaag atggcagata aattccgtgg
     8641 ttccattatg tttgtcgggc cattactcgc ccgcttcggt gaagctaaat ttcctcatcc
     8701 cggtggttgt gtcatcggcg ctggcggaag gccgatagat ttatttcttg aaggctaccg
     8761 tgcctttggc gccgaaatta aagaagaatc tggaaaattc tatcatatca aagctaaaca
     8821 cttgcaggcg gcagagtatt tctttccgct agttagcgtc acggcgaccg aaagcatgat
     8881 gatgacagct agtattattc ccggcactac catcttgaaa aattgcgcca tggaaccaga
     8941 aataagtgca ctcgccgatt atctcaatac tcaaggagca caaataagcg gcgccggcac
     9001 gcccatcatg gtcatcaaag gacaaaataa aattggcgcc ggagaattca cggtcatacc
     9061 ggacagaatc gaaaccgggt ctttcgctat tttagcggcg gccgctaatg ccaatctaga
     9121 aatcatcaat tgccaaccag agcatatcat gacgctctta aaaataatgg aaagaatcgg
     9181 cgtagatttt gaatatggtc gcgactggct aaaaattaaa aaaggcaagc gccctttgaa
     9241 agcgcatgat attaaaactc acgaatatcc aggattcgct accgatttac aatcgcccta
     9301 tgtcgtcctt atgactcaag cccagggtat atcactcatt catgaaacaa tttatgaccg
     9361 gcgtttgctc tggacagata tgctttcgca aatgggcgcc agtatcatca tgtgcgatcc
     9421 tcaccgcgtc gttattcaag gaccgacgcc attattcgga aaaagactaa ttagccctga
     9481 tttaagagcg ggcatcgctt tggttatcgc gggtctaatt gccgaaggac gtacggaaat
     9541 agacaatgtc taccagattg accgcggtta tgaaaaattg gaagaaagac tgcggaaaat
     9601 aggcgccgat atcagccgag tagaataaaa tttatgccta aaaaaattcg cgactggcta
     9661 tttatcctat ttataatctt gtttgtgatt attacttttt ttgtttccgt ttatgccgct
     9721 ggctatagcc ttaacaaacg ctggccgccg cgttttgacc aaatttttca aaaaaccggc
     9781 atgcttattg ttgacagcga accctctggc gcttctattt ttttaaacgg cgaacgccgg
     9841 cgcaaatcca tactgcttga tattggtcga agtgacgctc ttactcccca aaaaatcaaa
     9901 aatttgccgc caggagaata tatcttaatg ctagaaaagg acggctactg gcccctggaa
     9961 aaaaaaatta atatttaccc gggtcaaaca acttttgcag aagatttgat attattcaag
    10021 cgttccttgc cgctcaatct cagcctatgc gcgcctcaaa cattagagtt aagcccggac
    10081 aaccgaaggc taattctccc cgaagagggc acggctatta atttaaaaac ggaaacaaaa
    10141 agcgatttag gcctacaatt atcccaagga gacatccagt ggtctaaaga cggcgcgcgt
    10201 atacttttcg gaggaaaaat ccttaatctc gacaatggac aaagcaatga caccctcgcc
    10261 atgattggcc aagaagccaa taatttttat tgggacgaaa acggcaaaaa aatatattat
    10321 caagctgacg gccatattaa ttgtattctc accgacaata atacaatcag cactgtcctc
    10381 agcggcagcg attatgttgc ttacactgtc cgcggaaacc taatctatac aatcgaaaga
    10441 caaaatgaac aaatgtattt aaggctgtat agagccgaca atcatcttct acaaaatagc
    10501 atcaacctac cggccggtga ttatatattc aagcaagata aatatcatct caatctatac
    10561 gaccgcaaac aaaaagctct ctatctgctt aatgattacg cccagcaacc aataaacaaa
    10621 cagcttcgac cagtaaccgc ttggcaatgg ctcaacgcct ccactttaat gtggtacaat
    10681 gaatttgaaa tctattcttt tgaccttaat agcaatcgtc aagaattatt gataagagta
    10741 agcgagaaat taacaggagc cgtttggaac cgaagtaaaa attatataat ttatgcgaat
    10801 aacgacgaaa ttaaaattat taatttgaac ttagacaaac gtttgcctat cacgctgtta
    10861 aaagcggagg aaatttctaa cctcgcctta gatgaaaaaa atcagctatt atacttctac
    10921 gcaaaaatcg gacagcaggc cggcatctat aaattacaac ttcagtaaca aggtaatgag
    10981 ctcctttccg ccctcaaaaa gccacccctt ctcagggatg gctttttgaa gacggggttt
    11041 tttgatctta taaaaacttt ttcgtctaaa gagacttggc taggccacgc aagaagtctt
    11101 tcaggtcttc acctatttct ggatctttta tgcctatctt gatattagcc ttgattaaat
    11161 taaatttatt gccggtatca agccactcac cctctaactg tttaccataa agcacatgcc
    11221 cctgctccag catgacatca taggcgtctg ccaaacgaat ttctccggat ttgccggatt
    11281 gcatcgactt aaggatattg ataatttctg gtgtaataat gttgatgccg acttgcacta
    11341 gattagaggg ggcttcttcc agcgacggtt tttcgataaa tttcttaatt tcataaattc
    11401 cctctcccaa atccaaaccg tcgattaccc cgaactgact aacatcttta tgttccactt
    11461 cggataaacc gataaccgca tccccataac gttcataaac atccattacc tgcttgactg
    11521 gggaaatcgg cgcatcgtat aaagtatcac caaacatcac taacaccggc tcatcgtcac
    11581 gaacaagatg agaggcacac atgaccgcat gcccgtcgcc caaaggaatc ggctggcgga
    11641 cataagaaaa tttggccaga ttttcaatcg cttgaacctt cttcaccaaa tccagcttat
    11701 tcttttccac taaagtttta gacaattcaa aagaataatc gaaatgatct tcaatcgccc
    11761 gtttgccacg tccggtgaca aagattattt cttcaatgcc ggcggccgcc gcttcttcca
    11821 ctaaatactg aatcaccggc ttatcgacta ccggcaacat ttctttaggc tgagccttag
    11881 tcaccggcaa gaaacgcgtg ccgaatccgg ctaccggcaa aattgctttt ttgatttttt
    11941 gcatataatt atcagcggga cagccgttta gcgccatccc cacttcttaa aatatttcca
    12001 ccatgatttt aagtgttctt gggccagatg gtctttaaaa atggcgaggt accacggcaa
    12061 acgagcgctc ttgcgctgat ggtcgtggat aaccgttgct tccggataat agacaacttt
    12121 cttcccttgc tcatgggcgc ggcggcaaag atcgacgtct tcaaaataca taaaaaatct
    12181 ttcatcaaat aatttttcat gattaaaaag ctctcctcgc cttaccaata aacacgctcc
    12241 taacagccaa tcgacatttc tcgtctcctg atgattttca tgtttcatta aatatctatc
    12301 tattaatccg ggcaaatagt gaccgaccgc cgtccgcctt aataccggta aaaatatgct
    12361 ggggaaacgg gcgcaagaat actgtaaagt tccgtcagaa tttaatagct tagggccaac
    12421 gatagcgacc tcgcgattat ttgttaaata taaaacaagc ttcttaacgg cttcacgact
    12481 gaagacaaga tctggattag cgattaatat catttcaccg gagctggccg cgataccgag
    12541 attattaccg cctcccatgc ccaaattttg ctcgctctca atcaatcgga tgcgcgcatc
    12601 aaccgcggtt aaatcagcca gcttatcgcc agaagcgttt tccactaaaa taatctcata
    12661 ttccaggtcg cttaaatcag ccgtggctaa agaccgcagg caagcttcca attttaaacg
    12721 gcttttataa ttgacgatga taatcgataa gtccatatta ttgccggcag attcttatta
    12781 tcctgcgagc gatgaaaaaa taaaaagaaa acaaaggatt aactaaatat tttaacaccg
    12841 ggttatcaat ttcctgatac cagatgtgtc cggaaaataa aggtaatatt ttatagtcag
    12901 gtacgcagcg caaagtttgt gcctgttttc tggcttgaag cagatacgac caatttttaa
    12961 gattgaaaaa ataagcccag gctcttttct tttccttcag ccaaccggaa cgtaaagcga
    13021 aaaaactcat gcccagttcc attaccagca aaggcggtaa aaacaagatt aaagataaaa
    13081 ttgaataatt tttccaagcc gcaatcaagc ggtttctttc catccaataa tatttgctga
    13141 ccgaacggct gaattcatat tgatgatagg cgaccgcctt aaaagccaac aaacactgcc
    13201 aaccagacag ccagaaacgc cagcccaaat cctggtcttc attatacatc caaaattctt
    13261 cgtccaacaa gccgacggcg cgcaaagctt ccattcttag caaaaccgcc gccccggaag
    13321 gataagcgat tttttttacc tcctcgcctc tgtccaggaa tgtttgacga taggcgtcgc
    13381 aatagccgaa acccaaaaaa tgcgtaacat tacccaagct gtttatcatc tccctgtccg
    13441 agttaaacat taaacgcgcc tgtacggcgg cggcagccgg attagcctcg actatgcgta
    13501 ccaattccgt taaagccctg ggcgctaaag aagcgtccat gtttaataat aaagcataat
    13561 cataattttc cgccatcatc agacgcaaag catcgttatt acctttggcg aaaccatcat
    13621 tatttttatt aaaaataaaa cgagccgccg gcgcctggtc gcgcaaataa gcggctgatt
    13681 cggccgtact ggcattatcg acaataaaaa tatccggccg gcaagaagca tccaaagcgc
    13741 gaattgaagc taaacaaggc gtcaaaaaac gctgagcata atctttataa ttgacgagaa
    13801 taatgccaac tttcatagtc taagaaaaaa ttttagccaa gaacctcccc aatggccagg
    13861 cgctctttaa tacaaaacac tgccaagaag gctcccattt ctgaaaatat ttaagcattg
    13921 aatcttgaaa atatagctgc ttgttagaaa catcgacctg attgaagctc tggccgacat
    13981 aatccagaca acgggcaacc ggcgtatacc agacttcccc gccattctta taaacctgcc
    14041 ggcaaaaatc tacttcttca aaccaaataa aataccgctc atccaataaa ggcaaagcgg
    14101 aagcggaaat attctgccaa gcggcgcggt taatcataaa gaaagcgccg cgcacagaat
    14161 caaccctcgc cgcttggcta taatcaaaat ttttacaaag ataagaattt agccaagacg
    14221 gaaaaagatg aggaaacttc aaaactatcg ctaactgatc gcttaagcgc ggaaaacggc
    14281 gtacagacgg caatcgctct ccggattcat tttccaagcg gcaaccggca accaccgctt
    14341 gttgattagt tttcatccaa gccagcatat ttttaaccgt atccggaaaa accagcatgt
    14401 cgggattaag aagcagaata aagtctcctt tcgcttcctt aattgcttga ttattggctt
    14461 tggcaaagcc aagattttct tgattggcaa tcaacctcac tgtcgaaaat tctttagaaa
    14521 ccatttccgc gctgccgtct cgggagttat tatccaccac gaaaacctcg gccgacaagc
    14581 catttaaaga aggcaataaa gccgataaat tggcgcgcaa gcggtctttt accttccagg
    14641 aaacaataat gacggaaaca tcaagggaca taaattaaga tttttttact ttaattcttt
    14701 ttaatctggc gccaccaccg gccgtaatct ccaccaattt cttgtttacc cgaaaaatac
    14761 cagccaagaa ggcgacaagt tcggcgttag cctgccctct ttccggggcc gccttcactt
    14821 tcagacgcaa agtgccgtca gctaaaggtt cctgccattc tgtcttatcg gcgccgggcg
    14881 acacttttac cggaaaagaa attttcccct tctcgttaag ttttgcttgt aaatcggcaa
    14941 tatccataaa ttactgctta aacactaaag cgttgggaat atcgcggcca gggccgacca
    15001 tatattcgcc gttataaata agagccgaag aggaaccacc gtcaagattg agagcataat
    15061 ccatgcccat cgctttcata atcacagcca aatcatcgat agtcgcttgg tgggcaatta
    15121 ctatatataa ttcaccttta cccggattaa cgggatcatc cttataaccc aaagcgttgc
    15181 gccaatactt accggtcttt tgccctttat ccaaatccca atcaatcaag acattcatct
    15241 tgttttccac taagcgtggc tgattgccca aagccgcctg caattttacg ccatagttct
    15301 tttcaaaatc gtccacactt ttaaactccc gggaatcttt aaaataataa aatttattat
    15361 tcgtgtcaaa agctataagc gggccagtag tccaatattt taattggtca gcattgacca
    15421 tctctccaga caagctgtca taaaccggaa aaaaataata attgggccgg ccacacccgg
    15481 acgaagaaca aaaatagcta ccgttaacgc cggcaaaacc ttgattggca aagacataat
    15541 cccccaggct ttgcgcgcgg caaactccat ccgtacagac ttggctatca gccgccaaac
    15601 tcaaaatctc taaatttgga ttttttaaat caatccgcaa aacatctact ttaaaatctc
    15661 cacgctccgt agataccagc ttatgttcgt agccgctgta ttgattaatg ctttccgata
    15721 agccgggctt atgaatttgc gccagatttt gacgcgtgat tcccaaaccg agatttctca
    15781 ttacttggaa agcgtcagcc ggacggccga gataatattt ttttaaatcc agcggattaa
    15841 tataccaggc ttcgccattt ttttcgacct gcagaataat ccgaccggcc aaacgcttgg
    15901 ctaaatccaa attgccttca accggcatgc ccgcttgagc gatttcctga aattgagact
    15961 cgttaatacc caaaccaagg tttctcatca cctggaaagc gtcagccgga cggccgagat
    16021 aatagcgacg caaatccagc ggatttatat accaagcctc gcccttttct tccacgctca
    16081 aaacaattct cccggctaat ttatcagcta aagaagctgc ttgccctgaa taaaaaggca
    16141 aaaacaaaaa tataaagata ataatcagca aatatcgcca atatctcata aataataaca
    16201 tttttactta aaaatcaaac aaaatatgct tttattttag cctgttttta agaaaaaatc
    16261 aacgcaaaaa agattagtaa tatcttgtca atatttaaga aatctctcaa ctatactaca
    16321 atataatcaa agtaaaaact cattattaaa aaataaaaaa tatgagtgca gaagaaaaat
    16381 atattcgtcc ttcttgggat gaatacttta tgaagataac cgagatggtt ggctctcgcg
    16441 gttcttgcga ccgcggccgc gccggctgtg tcatcacccg cgaccgccgg attgtcgcca
    16501 ccggctatgt cggctctccc atcggcctgc ctcattgcga tgaagtcgga cacgagatgc
    16561 acactgttac tcatgaagac ggcagtacca gtcgccactg catccgtacc acccacgccg
    16621 aacaaaatgc catctgcgag gccgcccgca tgggcatctc tatagagggt ggcactttat
    16681 actgtaaaat gacaccttgc tatacttgtg ccaaaatgat tattaatgcc ggcattaaaa
    16741 aagtagtctg tgctcaagat taccacgccg gtgcgcgcag taaagaaata tttgaacaag
    16801 cgggtataga atttacatta ttgtcggaag aagtaatcac ctaccaagac atgaaataaa
    16861 aaatatgaaa gtctatttag atttattaaa agacgtaatg gaaaatggta aaaacaaaga
    16921 cgaccgtact ggtacgggca caaccagtgt tttcggccgg caaatccgct ttgatttaaa
    16981 cgcgggtttt ccccttctga ccaccaaaaa ggtgcattta aaatcaatta tctacgaact
    17041 cctttggttc ttaaagggag acactaatat taaatatctg aatgaacacg gcgtaaccat
    17101 ttgggatgaa tgggcggatg aaaacggcga tttgggaccg atttacggta agcagtggac
    17161 cgcctgggaa gcgcccaatg gagataaaat aaatcagatt gccgaagcgg tggaaatgat
    17221 aaaaaacaat cccagttcca ggagaattat tgttagcggc tggaatgttg gtgacttaag
    17281 aatgcttatt tccggcaaaa aaaccgcccc gcccccttgc cacagcttat tccaatttta
    17341 tgtcgacgcc gatgaccacc tatcttgcca gctataccag cgcagcgccg atttatttct
    17401 gggggtaccc ttcaatatcg cttcttacgc tctgttgaca atgatgatgg cccaggttac
    17461 cggcaaaaaa ctaggagact tcgtccacac tttcggcgac cttcatatat ataacaacca
    17521 tcgtgaacaa gtggctgaac agctcggccg cacgcccaga cccttgccaa caatgataat
    17581 aaatcccgaa gtcaaagata ttttttcctt tcaatatgaa gattttcaac tggaaggata
    17641 taatcctttg ccaggaataa aggctccgat agccatttaa acttatgata ccggttaccc
    17701 tcatcatggc cgttactgtt gatggtaaaa tcgccaaaga caaaagccag ttggcgaatt
    17761 ggaccagcca ggaagacaaa aagctttttg tcgaagtttc ccgagacttc ggcgttatta
    17821 tgatggggga aaatactttt aaaacttttc ctaaaccatt gccgggaaga ctgaatgtcg
    17881 ttttctcgcc agacggcggc gaaaatattg agggtctact caaatgggta aaaggcgaac
    17941 cggaaataat actagcggaa ctggaacaac ttggctacac caaggctcta ttgggcggcg
    18001 gctctttttt aaacactcta tttttgaaga aaaagttgat taacgagatt attttgacag
    18061 tggagccaaa aatattcggc catggcctat cattatttac agaaatctgc gaaactaatt
    18121 taaacttatt ggattgccaa aaactaaatg aaaatacttt aatgctaaaa tataaagtaa
    18181 tttattctta gattttatgc ctaaactagt tctcggcttc gtcggtaaat tagccagtgg
    18241 caaagcggtc tgtcaaaaat atataagcga aaaatataat gctggcagcg cccgtttttc
    18301 tacagcttta cgcgacatct taaaccgcct ctatctgccg atttccaggg aaaatatgca
    18361 aaatctttcg tttgatcttc gcaaacgttt tggtagcgat attttagccc gtgtcattgc
    18421 cgaagatgtc aaaaatgacc cgcataacat cgtcgtaatt gacggcgtca gacgtttagc
    18481 cgatatcagc cgtttaacag agttgtccaa cttccatcta attagcatca acgcctctcc
    18541 ggaaatacgc tacgaacgga tgaaattacg caatgaaaac gctggcgacg ctgacaagac
    18601 ttttgaggaa tttttagaag acgaccggcg cgaagccgaa ctgcaaattc cagaagtaat
    18661 ggccgccgcc agtttcaatc tggataataa cggcactttg gaagaattat attcccagat
    18721 tgacaaaata ctggaagaga tacaaaatta aacaagacgc agcattagga ggagtgctga
    18781 ggctttaacc caaagcactc ttttattatc ccaaaagata ggaaaaaatt atgtctaatt
    18841 attcaggaaa atttattgtg attgacggca ctgacggcac gggcaaaact acccagttgc
    18901 aaattttagc gaatagactt catcatgaag gctatgaagt agagttagcc gattttcctc
    18961 aatataatac taaatcagcc ggcatggttg aagaatactt atcaggcaaa tatggtgaag
    19021 ctaatgatgt taacccctat gccgcctctc tcttttattc ggtcgaccgt tttgacgcca
    19081 gctttaaaat cagagaatgg ctagaacaag gaaaggttgt tatctgcaac cgctatattt
    19141 ccgccagttt tgcccaccaa ggaaataaga ttaaaaatgc cctggaaaga aaattattct
    19201 ataactggtt gagcgaaata gaatataaag tcttcaatat acccaagccc gatttatatt
    19261 taattcttta catggaaccg gagatctctc aaaaactggc tcacaaccgc cgacgtgaag
    19321 actggaaagg aaaaacaaaa gatattcatg aagataacct ccaacacctc cgccaagccg
    19381 cccagactta tttagaaatt gcccaagaaa tgcccgattt ccgcttaatc agatgcacgc
    19441 gcaagcaaga aatattaaaa cgagaagata ttcattatct gatttggctt tatgtaaata
    19501 gaattttaaa ctccgatcaa accaccccaa aaactccgga cttacaagca cttaacgata
    19561 ttttgattaa taaaagcaaa ctaacgccaa atctgccgga attaatagac gtaccgcgcc
    19621 tcagcattag cgctgccgcc agtgttttgg gagaaagccc aaccctcggt ctgcctgctc
    19681 attcagacaa agatgagagt cccgctatcg ccgataaagg aattaatcga ttgatggttg
    19741 aacgcttgct tcctgaagcc aaattgccag aacgggctca tccttacgac gccggccttg
    19801 atctctttgc ggccgaagac tgctccatcc ctccttacgg tcaagattta atccccaccg
    19861 gcattcgtct agccatacct gacggctacg ttggtctaat ttgggataaa agcggcttgg
    19921 ccagccaggg atttaccact atgggtggcg ttattgattc ccgctaccgc ggagagataa
    19981 aagtgatatt taaaaatcta agcgaggata tctaccatat tcaagccgga caaaaaattg
    20041 cccaactcct aattcaaaaa atagaactgc cggatgttca agcaggtaaa atagaagacg
    20101 acagtcaacg tggcggtggt ggttttggta gcaccggttt attctaataa aggccttaaa
    20161 ggcctttttt gataaatagc caagacttct ttcctaaatg ctattttttc gttaaaatag
    20221 agttatatga aatacgacgt tattatcggc atggaaatcc acgccgaatt aaaaaccaaa
    20281 agcaagatgt tctgttcttg ctctaatgac agcgatggca aagcgccaaa tacgactgtc
    20341 tgcccgattt gtctaggtca tcctggcact ttgcccgtac caaataaaca agcaatagac
    20401 tggaccttgc ttataggctt agctttaaat tgtcaaatta actaccattc aaaattcgac
    20461 cgtaaaaatt atttttatcc cgatctccct aaaggctacc aaatttccca atatgatcaa
    20521 cctttctgct atcaaggcag acttgatatc ggtaacaaag acatcgctat cacccgtatc
    20581 cacctggaag aagacaccgg caaattattt cacccccaaa atagtaaaga cactttaatt
    20641 gatttcaacc gcgccggtac gcccttggta gaattagtaa ccgcgccggt aattaaaaac
    20701 gccgaagaag ctaaaaactt ctgtcaggcc taccaaagag tattgcgctt cttggaagtc
    20761 agtaacgctg atatggaaaa aggcgaaatg cgctgcgaag ccaatgtcag tttacaaaaa
    20821 cctggtactt gggtctacga aaatggcttg attgttgcca ctggcaaaaa aaagctcaac
    20881 gctaaggtgg aaatcaaaaa tattaattct ttccgagccg ttgaaaaggc aattaattat
    20941 gagattacca gacaaagcga gatgttagat aataaagaag aaattattcc cgaaaccaga
    21001 ggttgggatg aaaaaaagaa tgcgaccgtc gcccaaagaa agaaagaaac agccgccgat
    21061 tatcgctatt ttcctgaacc ggacattccg ccaattatca tcgatgaagc ctggttgaac
    21121 caactaaaac aaaacttagt ggaactgcct gatgccaaac aaaaacgttt tcagcgacaa
    21181 tatggtctca aaactgaaag cgctgccatt atcgccaacg acaaagaatt ggcctctttc
    21241 ttcgaaaacg ttgtttcaga aatgggtgct tggattgaag ctaatggtga tgattttgaa
    21301 aggcaagata aaaatgccgc ccaaatggtc gctaactgga ttaacagcga actgcttaaa
    21361 aacctgaata ccgataaacg cgctatctcc gatttaaaaa tgacagctga aaattttgcc
    21421 gaattaatgt cattaatatg gcaaaataag attaattcca acgccggtca gagaatctta
    21481 aaaatcatat attgggaagg cggcgacccg agtgatatta tgacgaaact tggcctcgaa
    21541 cagttagata gcgaggagga gctaacaaca gctattaaaa aaataatcaa ttcaaatcca
    21601 cggcaggcag ccgaatacaa gaaaggtaaa aagccactta tccaattttt catcggcaaa
    21661 gttatggccg aaactaaagg taaggctaat ccggaaaaag ttaaagattt attagagaaa
    21721 attcttaacg aatagctaaa aaattaataa actccttcct gactttataa aaaattaaag
    21781 ataattatca attaatactt ccgcctcgct taatacttcc gcctcgcttt gccccctcag
    21841 ccaatgaatc tttcttccct gtctttccca gcgcttaaac caagtcttct ggcgtttagc
    21901 gaaacgatag gaagcgtcgc ctaatttctg caccatctcg tcataatcaa gccgccctaa
    21961 aaggtaatag ctgagaaatt tatattctag accaaaggat attaatcttt cccagctaac
    22021 accgctatta tgaaggcgtt cgacttcgct aagcatatct tccttttcta aacgattctt
    22081 aatcctttta ttaatccgct ctcgcaaaat atcgtcggga aagtctaaac ctagcactaa
    22141 aaaatcataa ggggaatcct gtttattgct taggtcgcca tctttttcta caatttccaa
    22201 ataacgaacc aagcgacgcg gattattctt atcgctattg ttaagcttaa gcgcaaattc
    22261 cggcttcaat ttttttaaac ggccaaacaa ttcatctttg gttaaggcct caagctcccg
    22321 ccgtttttct aaatcgggac taattttgct taaatcataa ttatcgacca gcgcctgtaa
    22381 gtatagaccg gtgccgccga caataatcgg caattggccg cgattaataa tatctgatat
    22441 tgcctctaaa gctagtttct gatattgagc gaggttaaat tcagtattgg gagaaataac
    22501 atcaattaaa tgataaggga tatgtttgcg cccgacttta tattccgcta aatccttgcc
    22561 ggtaccgata tccatacctt gatagacctg gcgactatcg gctgatacaa tttcgccttg
    22621 atatttccaa gctaaacgca cacctaaagc cgttttgccg acagcggttg gtccgagcaa
    22681 aaccaaaacc ttgggcttat taagattgat tgcttttggg gacatataat aaattaggtt
    22741 atttttctag gataaatatg ctttgtttta tagacctgac gccgggattg cgtttaggcc
    22801 ttttttattt tttcaaaacc ccttccaaac caaaatcttg aaccttttga attttcaccg
    22861 acacaaaatt accaataata tcttccggcc ttacctctga cggggctgta aaagaaactg
    22921 ttttgaaact tgaggtttta ccataataac cgccacgcct attttttcct tctactaaaa
    22981 ctataaccgt ccggcgccaa taagcgcggt tagccttctt tccactcttt cttaaaattt
    23041 ctgttaatat ctgctcccgc cgatgtttct cttctcggct aacattatct ttcatatacc
    23101 aagaagccgt tcccggccgc gggctatact gggcgatata agccatatca aactgcaaat
    23161 ttttaaataa tttaacggaa ttttgaaact gtttctttgt ttctccggga aaaccgacaa
    23221 tcacatcagt cgtaagggca atccctggtt ttgcttttct aatttttttt attaaagaag
    23281 aaaaatgagc ggccgtatag tggcgattca tcgcttttaa aatttcatca tcacctgact
    23341 ggacagccac atgcaagtga tggcaaattt tatcagaaga agctaaaact ttaattaatt
    23401 ctggcgataa atctttagga tggctagaag aaaaacgcaa ccaaaaattc ccaggcaaag
    23461 ccgctaattt ggataacaat tgaagaaaat tcgtgcgtcc gcttctatag ctattaacat
    23521 tctgagcaat taaaattatt tctttatatc ctctttttag caaagccttt gcttctttga
    23581 taacatccgc cgctggacga tacacttccc gaccgcgggc ataaggaacg acgcaatatg
    23641 aacaaaaatt attgcagccg ttgccgatgg gtatataggc ggtaaaagta ctctgatatt
    23701 tagggatgat agtcaaatat ttttctccgc ttaattcacg aacggcatca gtacttagac
    23761 ttaattgccg gtgtgaatct ttcagtaaag caaaaatatt aggcaattca ttaatgggca
    23821 taaataaatc aactttgcct cgcaggcgcc gtttcacatc ttctcgacgc gccaaacaac
    23881 cagtgatgac tacggccgct ttgggattac cctgacggat acgattaacc aggccgtaaa
    23941 ctctatcttc ggccgactgg cggacgccac aggtggtgag aacaaccaaa tcgacgccca
    24001 accaagaaga ggaggcaaca tagccacgct tctccaaaaa agaagcaata cgttcgctgt
    24061 ccgctttatt catctgacag ccaatagtga taagatgata ttgcataaat ataatttaac
    24121 tacttacttt ctaccaaggg ttctatctta tctaaacgat acaaagtaaa ttgaccgcga
    24181 tgaacaaccg gattgataat aaagccaaat tggggtagac gccgttcgtt gagatagctt
    24241 aaatccttgt taggaaaggt aaaattatag taataaatcg gataaaagcg caatattttg
    24301 cctaagctct tattaacatc agcgtcggta agcaaagaaa caacaacctt tctttgcgga
    24361 aacagctgct tgtcgtgata ttgggtgaca ataatcgccc gctcttcagt caacccgata
    24421 atagcttgcg cgttagtttt atcaacaaaa taattgtgat ataaagtcgc caagccttct
    24481 tccgagccga aaaaaacaaa ctctaaattg acaaaaatta gaaagcaggt aatcagggcc
    24541 gataagccga tagcgagaaa gcgacgccaa cgacgtttta aagaaaaatc aaatctcggc
    24601 caggagatag aagtaataaa tctagataca gaaacaacaa acaaagacac aaggggaatg
    24661 gccataatat acatcggcag ccaataacgc gtataggaat taccgatagt aaatctttta
    24721 ggatcaggat tgtcgttaaa atgccagctg ccataataaa aaactaaaat taacgataaa
    24781 actagccaag taataagata taagtaagca ccccgattcg gtcgccggat tattctgtaa
    24841 ataaaaatta aaaaaccggc aaaagcaaaa gccgataacg cgggaaacat ttttaacaca
    24901 tagtagaaaa acatctttaa agactggtag ggttgatagc cgaaataaaa aacattatca
    24961 attaatgttg ccgccgctaa acggtaatgg ctgaaatctc cgcgcacgct ggattgtaaa
    25021 aattgtccgc ccacttgaga aatttccgtt aaagaacggt tcatttctcc atagcctcca
    25081 aaaaaaggag aagcatacaa aatctgattc caatagaata tcgggagcaa ggctaaaact
    25141 aagccaccaa gcattaaaac taatctcgtt aaccctaaac gacgggcata aaaaagccaa
    25201 gcaataaaaa gcaccggccc cagccatagt aattccgaag aacgggtgcc aatcgcgccg
    25261 ccgaacatta ggccggttaa aaaagaaaaa atataagtta aacaatcttg agccgacaag
    25321 tgccatgtcc aaaatttacg cttatcactt gtccgcgacg gcaggctcaa taataaaaaa
    25381 taagcgccag ccaacaaaaa aaccagaaac aagacattat gaaacatgct tcgcgccgaa
    25441 taaaaaaaat agacaggaaa tgaagccagt aaaaaagcgg aaatcaaagc cacctgccga
    25501 ccgaacagtc tgcgcacaaa agcatagaaa aaaataatac caaaagcggc aaaaagtggg
    25561 gttaaaaaag gaattatcgc cgtacccaac caagaggcta tctggccata aattaaaatt
    25621 atacccaaaa aactgacggg cttaagccag ccgttatcac tcctgatact ccgaggatgg
    25681 acaatttcct cagcgattaa attagccggc tcaaagacgg cgatactgcc ggtctcggca
    25741 tagtgcttgg caaaaaaata attggcagtt tcatcgggcg ataaaaattt tacataatca
    25801 gaactttgtg ttgcataatt aaaccaagcg gtcgccacca aaaagacgaa tgccaataaa
    25861 aatactaaaa aataatcaaa attattctta aataagcgaa atttaatcat aaaaataagg
    25921 cataatctcc tctttttaat agtagccaaa attgcttgaa taagcaagaa tctgctatga
    25981 tatattatat gctaaaattt aaattttccc ttacttttga tagtctttgg ctctggttaa
    26041 tgctgtttac cagtagcgtc gctttagttt attttctcca tgctttagat attatcggcc
    26101 tgattgccgc ctttttgatt gccactatta tttatgcggt ctggaagaaa caaattaaag
    26161 agcaaatatc aactaaagtc tccattaaaa attcaaagta tttttggttc ttctctttaa
    26221 tgacgcttac catgattata atcatggtaa ttatggccgc cgggaacgcg actgataaag
    26281 cgataatttc tccctggacg gtcatcaatc cttggttttt tgttgtcctc tttgccgcta
    26341 atctatctct aattggcgct ttaagccaaa cagccaaaag tttgttcaaa aatattctgc
    26401 tgggactata ttatctggaa attttttctg tggccgccat cgtctatcgc ctcggctacg
    26461 gcttcgatcc ctttattcat caagcggcaa tggctgaaat aaatcgtatc ggctttattt
    26521 taccgaagac gccctactat atcggacaat atggcttgat tatcgccctt tataaaatca
    26581 gcggcctcag tttagctctt atcaaccaat atttcctgcc gcttgtaagc gctttatccc
    26641 taccctggat tttaggccag ctccggcaaa aatcttcccg accggaagct tggttggcca
    26701 taatattgtt gactctcctc ggcttttccc cttttattct gagcacaccg caaaacttca
    26761 gctatctgtt gttattggcc acaattattt tcatttaccg acaaaaaaac gaagccttga
    26821 taatcagcac cgccttggcc actttggccg ttcatccttt ggccggcatc cccgccattt
    26881 tagccgccgg ctggttcatt atccaaaaac gctggccacg gctgaaaata agtcgttttt
    26941 ttaaaaatcc aataataatc tttggagtcg ctctgctggc tttctgtttt tccctctggg
    27001 cagttgccgg cttcggttct ttatcatggc aaaattttaa tctcagcctc ttaaatcctg
    27061 tctttaaaaa cagtgaaaat tattggctta acctcggcta ttttttcatc aataattatt
    27121 tctggctaat cttgggaatg gtggtaatta taatttttaa acgccgacaa atttggccag
    27181 aagaacaact gaaaacggaa aataatatcc gcctcctgag ttctgcctcc ttagccgcta
    27241 ttaccgccta tttaatcagc cgcggtttta ccttcaataa tttaatcgcc tacgaacaag
    27301 aaaattatgc atcgcgatta cccattattg ccctcattat cgctctgcct cttttttatg
    27361 aattgtttag atacttaagc caacaagctt taaaaatgga aaaaagcttt aaaataatag
    27421 ctgctatcgg cttagcctta atgatgaccg tgtccgttta tgcttcctat cctcgcttcg
    27481 ataactatta taattcccgc ggctattcaa ccagcgccgc cgatattaaa tctgtgcaaa
    27541 gagcggaaga acgagctcag ggagaaaaat atattgccct agccaatcag caagtaagcg
    27601 ccgccgctct gcatgaattc ggtttccatc atcgctatat aaaaaaagaa ggacaagaaa
    27661 tatatttcta tcccatccct accggcggtc ctttatacca atattattta aatatggtat
    27721 ataaaaaagc taaccgccaa aatatgttgg cggccatgga ttttgctgat gttaatagag
    27781 cttacctgat tatcaataaa tactggtggg cgagcgacaa aattatcgcc gaagctaaaa
    27841 tgagcgccga ctactgggaa aaaatcgaac aaggagaaaa ttatctgttc gagtatcgcc
    27901 gataaaaagt ccaggttaaa aaattaggga tataaaatat ttaaatatta agcaaattta
    27961 cagcataaat tctttaatat aaaaccgcca aatggcggtt ttttagataa atattaatat
    28021 taattattta ctctcctgat gattaaatac cggaccgaca gaaaatacaa tcttatcact
    28081 cacaggatga gcaatgataa ggaggcgagg agagcctaag ctttccttaa tattagcgcc
    28141 taaagaatta ttacgccaag ataaggtttt ttcggccggt tcttcaacca aataagcacg
    28201 aacaaaatca cgaataaatc gattatcatc agcatttaaa tcttctccca ccgcttgttt
    28261 ggcgataata gctctaagcc cggctagttg gcggccggct tcacggatat tattggtggc
    28321 caaactagac ttttcatcca aaccgattgc ttcgaacatg cctaaaatca tctgattatg
    28381 agctaaaaga cgatcaagat aatcaatatt aggctcaacc caattatatt cggaaaaact
    28441 gacagttgct cctaaatttg aattatttgt actctggcta cgcagactga ttttatcaag
    28501 aggcaattgc atatccgtcc aagaacctaa aactgaatta gccatatgag cgcgccaatc
    28561 ttttgagcgc agatatgcgg gcagacgggt gtcagaaata tttaaataat aacgattggc
    28621 cagtaatgaa gaccaataat tattaagatt ttcgttcatg ccaccctcga ctaaggggcg
    28681 taaattttct aaagcttcct tataattagc ataatttcca ttttctaaaa atagcggtcc
    28741 gttccaagct ggatacaata aactcaatat gtcctgactg gaaccctgac aacgctgata
    28801 ttttttttga accaaacaag cagtaacatt attagccact ggcttttctc cgccctgata
    28861 aacgccaact tcgggatatc ttaagtgtcc aaaaataaaa tcatttggcc aataaaaatc
    28921 tgccaaaaac tgcaagccag caattttata accggccata gtcttcaaac ttaagccccc
    28981 ctgcattggc accacttccc tctgaaggag attttggcgc aatttttcca tatttgcctc
    29041 tgcttctgga ttatcttccg caaacagacg ggtaacatca aaatcatcgc cgaaactatt
    29101 tttcaggcca tcacgataat gtacataatt ccaaccatcc cttaaaccct tgaaaaaaga
    29161 aataatttta taaatacgcg cccattcgct cttcaactct tgattattgg cgaaatcgct
    29221 agcaattaaa gaagcggcgg taaaattaat tcgccaatca tctttatcca gtaaacactc
    29281 ggggcaagac tcgtcccgat aattaagagg aaacacggaa ttaagccaga ccgctgtcag
    29341 gtagaaattt tgtaaacggg cattattctt atattcctgc ggtattatga atgatttata
    29401 atcacgatca tataatagaa cgggagattt ctgctgttct ttagcggcgc gaatcagctc
    29461 cagttcttta gtaacatcat cacttaaata agccggtaaa gtaaaggaaa atttttgctg
    29521 atcccgaccg ctgaattttc cggagttaag attattggca gagtcaacct gatccaaacg
    29581 cggctttaat aattctaaag acacggcaaa aaaagccgcc tctaagcgct ccccttccag
    29641 aacacggtca ttgatattgc cagaaacttg taaatgattt tcataacgaa tacgcgcgcc
    29701 ttcgtataaa gtacggttaa tattccagag actttcataa aaaacacctt cttcaatttc
    29761 tttaaaagac attttcaaaa tactttgata ataataggag atgaaatcgg cgctgataaa
    29821 taagggtatc tgcttatcat caagcaaagc gcctaaggcg tagaaatcat tagcttcttt
    29881 gggccaagga ttgtctaaaa tagcaaagcc gttcttattc aaagattcca aactggaatc
    29941 taaatttaac ttacgcgaaa catcataata attagatacg tctaatttca cgtttatcgg
    30001 gacggcatat tcaggaaatt taaaatcatc aatgatatta tcgggctcat gataaaaata
    30061 agaaaaatat aaatattcaa cagcattagg ctcttcggcc gtattagagc cttctttttg
    30121 tccatcgatt ccttccaaat gaaaatcatt gagccgagcc ggggctgaag atgaggcttg
    30181 actatcatcc ccggatggct gcgaagatga tttaagacgc tgagaaagcc accaaatacc
    30241 tgcccctaaa gcggcaataa taataattgc caaaatcaag attaatatct tcttcttagc
    30301 tccagattta tcttcccccg cgccctgatt agccgcttca ataaaattgt tggcgccact
    30361 ggaaggtatt ttgacttcct caaacataaa attaaggcaa atatttactc ttattgcgta
    30421 aatgttcgct ataggtagta gaataaataa tatctttact gccggcggga gttaaaaaat
    30481 aattataatt ggtgcttaat ggataaatag cggcctcaat cgctaaaata ccgggattac
    30541 taattggtcc cggcggtaaa cccctatatt tataagtatt atagagagat tctgtctgga
    30601 tatcagcgga tgaatactga gatttattaa cccctaagac ataggcgaga gtggcgcatg
    30661 attgcaaagc ctggccatat tttatcctgt tccaaaaaat tcctgaaata attctagcat
    30721 cacgattatc accactttta taatttatcg gcgcctcttt ttccaacaaa gaagccatgg
    30781 tgacaatttc ataaatggtt ttaccctgct ttttaatatc ggcacgcatc tttggtgtca
    30841 attgattgtc aaaattctga agcatcttaa caaccaattc tcgcggagaa gaggtggcat
    30901 agatacggta agtatcaggg taaagataac cctcaagacc gcgatttttt ggcttatccg
    30961 acaaaaaaga aaagtcatca gaaaaatctt caagcggcgg caaatctttt tctttgttgt
    31021 aatctatctg cggaaagccg acaaactcca aaaattcttc actttgccat atcccttgac
    31081 tttcaaagta ttgccccatg tcgcggctgg tccaaccttc aattaaacga acagtttttt
    31141 ctggtcgtgg ttctattttt ttatcttcag aagaactaaa aatattatcc aagcgcgcca
    31201 gcaaaccgtt aacagggcct agagaagaca acaagctccg actaaaaacc aagctaagga
    31261 caatagtcgt taccaataaa cttaagacta ataatataaa atagcgaaca atttgacgca
    31321 tagcttatag attaactttt aattttggct ttcttggccg ccgctttcgc aatcaaaaca
    31381 gaactggccc gtattttttt acccaagccg tcaaccattt ttatcttata tttttggcaa
    31441 atcggatatt ccggaacatc gccgaggctg tgacgatcgc caccattagc aaaaatatcc
    31501 ggcttcaatt tagctagaga tttacaaacc gttttatcac ggtcaatcga cacaaacact
    31561 tcatccacgt cacgcaaagc agaaataatt tttactctat cttttaaact cataaaagga
    31621 acactccctt taagtttcac ctgaaaatca ttattgatta tcgccaccaa tttatcgcct
    31681 aatttttttg cccgcgcaat catttctaag tgcccgacat gtaaaggatt gaaataacca
    31741 gaaacagcga ctactatcgg ctttttagga atattagtag tttttttatg actatacata
    31801 taaacaaaga ttacttttat gataatatta tagcaataaa acccaaaaat tcaaaacagt
    31861 tgataattaa taacctcccc ccttttaaaa aagaggcgca atgagcgcct ctttatcttc
    31921 aatactaata ttaataatta catcatgtcc atgtccatgc cagacatacc gccaccatga
    31981 ttgtggctat tgtgcgcttc cggtttatct gtaataacgg cttccgtggt taaaaacatc
    32041 atagcggccg aagcggcatt ttctaaagcg gaacgaacca ctttagtcgg gtcaacgata
    32101 ccagcggaaa tcatctcgac aaactcgcca gtggcggcat tatagccgcg gacctcttta
    32161 gattttttat tctctgcaat aatcctttgt aaaaccaaag aaccgtccac tccagcatta
    32221 ttggcaatct gcttaatggg ttccaaaacc gattgattaa ttattttttc accggcgggc
    32281 aaagaaccac catctttcat ttccttttca aagccggcaa aatccaaacc ataacaagca
    32341 accgccaaag ctaagccgcc gccagggaca actccttctt caaccgccgc tttagtagcg
    32401 tttaaagcgt cttcgatgcg gtatttcttt tccttcatct ctgtttccgt cgcagcccca
    32461 accttaataa cggccacgcc accggaaagc ttagctagtc tctcctgcaa tttttcttta
    32521 tcaaaatcag aatcacttaa ttctttttct ttctttattt gagcgattct atcctcaatc
    32581 gcttttttgt cgccagcacc gtcaataata gtggtattat ctttagaggc caccacgcga
    32641 cgggcgcggc ccaaatcttc aatttccgcc ttatctaatt tcaagcctac ttcttcagaa
    32701 attaagcgcg caccggttaa aacggcaata tcctccaaca tcgctttgcg gcgatcgccg
    32761 aaaccaggag ctttaatgcc taaaacattg aaagtgcctc tgagcttatt aaccacaaaa
    32821 gtagccaaag cttcaccttc aatttcttcg gcaataatca ctaaatcttt tttaccagat
    32881 tgagcaatct tttccaataa aggcaagatt tcctgcaaag aagcgatttt tttgtcagtc
    32941 accagaatat aaggatcact catttctgct ttcattgttt cctggttgct aatcatataa
    33001 ggagaaacgt aacctttatc aaactgcata ccctcgacca cctctttttc aatgccaaaa
    33061 gactggcctt cttcaacggt aataacgcct tctttaccaa cgctatccat cgcttcggca
    33121 ataattttgc cgatttcttc atcattagcg gaaatagcgc cgacttgggc aatttcttcc
    33181 ttagtgctaa tactcttgct catctgtttt aaagcagaca caatctgagc aactctaacc
    33241 tcaatgcctt tacgaatttc gattgggttg actccagccg ccaccaattt caaaccatta
    33301 gtaataatag cctgagctaa aacggtagcg gtggtggtgc catcgccagc ggcatcgcta
    33361 gttttagctg aagcctcctt cacgatgcta gcgccgatat tttcaaactt atcttcaagc
    33421 tcaatttctt tagccacagt gacgccgtct ttagtaacaa tcggggagcc atagctgcgg
    33481 tcgatgacta cattgcgtcc ttttggacca agggtaattt taacggcatt agctaactga
    33541 tccgcccctc tttttaaagc taagcgcgcc ttttcgtcaa aaattatttg tttagccata
    33601 tatttataat aattaattat ttagagttta ttcaataata gcgacgacat catcttcgct
    33661 taaaatcaaa taatcttccc catcaatctt aatttcatca ggagaatatt ttttaaatac
    33721 gacttggttg ccgaccttaa gactcattgg tgataattga ccgttatccc taatctttcc
    33781 cggtccgacc gcaatcactt cgcctttttc cggacgctcc ttatcagccg tatcggggag
    33841 gacaattcct gatttggtaa cctcttcctg aacggccggc ttcacaatga cgtgattaga
    33901 taatggtttt aatgtcataa cacagggagc taaaataatc cataaaacaa atcatgtaaa
    33961 atgttttaaa ggaaaatttt agctgatatt aataaataat attaaacata tttattagca
    34021 ctcattagca ttgagtgcta agtctaaacc tattttatag tttttgccaa agcctgtcaa
    34081 gtctaaatga aagaaatccg gcaactagcg ataaatgaaa aaatatatta aactaaaact
    34141 atactaaacc tctatgaaca tccttcattc tctcatactt ggcattattg aaggtataac
    34201 tgaatttttg ccaatatctt caaccgccca cctgataatt accgccaaag cgcttaaaat
    34261 acctagcagt gattttttgg acagttttct tatttctatc cagctgggag cgattctttc
    34321 ggtggctgtt ctttatgctc aaacaatcat tcggcaacct cgcttaattc taaaaatcac
    34381 cgccgccttc atccccactg caattattgg tttagcttca tacaaactgg caaaaaccta
    34441 tttatttgaa agtttacctt taatcgctgg cgctttatta gttgggggca taatacttat
    34501 cgttttggaa aaatattttg ctcagcacca gcgacagaac gatgaacaaa gcttggcacg
    34561 aataagttac cgccaagcag cgtatattgg catctttcaa tctctagcga ttgtgccagg
    34621 agtttcccga tcagccgcca ctattatggg cggcttagcc ttgggcgtgt ctcgaaaaaa
    34681 tattgttgag ttttccttct tgcttgcttt gccaacaatg gcggcggcta cggctttaga
    34741 tttatataaa accccgccat tattacaagg gaatgaagcc ataatctggc taattgggtt
    34801 agcagtttct tttattactg ctattttggg aataaaattt ttcttaaaat ttatccaaaa
    34861 gaataacttt caagtttttg gctggtaccg aattattttg ggcataatta ttttcgcttg
    34921 gatattgtta taggcaaaac gctttactat aattaaaaaa tgatgaataa tatgctcgca
    34981 aaactaaata actctttctt ttttgacttt tactttaccg gttttagtcg gccaatttta
    35041 cctatcatca attaatcgat gatttaatta agatatcaca aaaccggcca gaaacaggcc
    35101 ggttttttat tagtaactat ttaatatttt tgagcttacg ccatttatgc aaagcccgct
    35161 cacgagcaac ttctatttcc agtttggcgg ccacggcgga caaatcatag ttatcttgac
    35221 tcatggcttc ttctttagaa acttccgctc gacggcgagc ctcagcgata atattttcat
    35281 caagttcttg ggctctctcc gccgtatcgg ccaaaataac aatcttatcg cgcaatacct
    35341 caataaaacc cccagacacg gaaataattt cggtctcctg atcaaccgtt ttaatttcca
    35401 aaactccagg cttcaaaatt gaaactaaag gaatatgatc cggtaaaacc gtaatctcgc
    35461 cagactgagt aggaacagtc acctgaaaaa cctcctgttt aaaaacagta cgttccggag
    35521 taacaatttc aaattttatt atctttctat cggccataat aattaagcat tagccgccgc
    35581 cacttcctca ataccgcctt tcatataaaa atcatcttcc gagcgatcat catgtaaacc
    35641 ctcaaggatt tctttaaaac ccttaacagt ttcgctcaac ttgacgtatt tacccggacg
    35701 accggtaaat gcttcggcga cagtgaaagg ctgggataaa aatctttgaa tcttgcgcgc
    35761 tctagccacc aacaatttgt cttcggctga taattcttcc atgcctaaaa ttgcgataat
    35821 atcttgtaaa tccttatatc tttgcaaaat cttctgaacg cctcgggcaa cttcatagtg
    35881 atcctgtcca attactttag gatcaagaat gatagaagat gaatctaaag ggtcaaccgc
    35941 cggataaata ccgagttcgg ataaagaacg ggacaaaacc acggttgaat caagatgacc
    36001 gaaagtggta gccggagcag gatcagtcaa atcatcggcc ggcacgtaaa ccgcctggat
    36061 agaagtaatg gaaccatcct ttgtcgaagt aatacgttct tgtaattcac ccatctccgt
    36121 ggataaagtc ggctgataac caaccgccga aggcatacgc cctaaaagag cagaaacttc
    36181 ggaaccggcc tgagtaaaac ggaaaatatt atccacaaaa aacaaaacgt cttgatgttt
    36241 ttcatcacga aaatattcag cgatagataa accagataaa gcgacgcgcg cgcgcgctcc
    36301 cggaggttca ttcatctggc cgaaaaccat agacacttta tcaagcacac cgctatcttt
    36361 catttcatga tataaatcat taccttcgcg tgtacgctcg ccaacgccgg caaaaactga
    36421 atagcctccg tgttcgctgg caatattatg aataagttcc aaaataacaa ccgttttgcc
    36481 gacaccggca ccgccgaaca aaccgacttt accaccttta acaatagggc aaataagatc
    36541 aatgacttta ataccagtct ctaaaatttc cacattacta gactgctctg taaatttagg
    36601 agcaggtcta tgaatactat atttcttttc tgtttttgga gtttcaccac catcaaccgc
    36661 ttctcctaaa acattaaaga tacgccccag agtttcctga ccaacaggaa cgctgatagg
    36721 cataccagta tcaagcacct ccgcgcctcg cttcaagccg tcagtcgaac ccatagctac
    36781 cgttctcaca atatttgagc caagatgctg ttcaacctcc aaaattagct tcttgccatt
    36841 taaatcaacc tccaaggcat tgtaaatagc ggggagggcc ttatcaaaat aaacatcgac
    36901 aaccacgcct ataacctgct tcacgctacc taaattattg tttttttcag tcataaattt
    36961 ataaattcaa aattcaaaat taaaatttaa ttaagcagag cgttagcgcc agcactgatt
    37021 tccgcaattt cagcagtgat acccgcctgg cgggctttat tataaaacaa agtcagttcg
    37081 tttaacatct ccccggccgc ttcagtcgct tgatgcatag cgctcattct agcactatgc
    37141 tcagaagcat tagcctccaa taaggcttga aaaacctgaa cttcaagcag gcgcggaata
    37201 atattatcta aaacctcgga aggactgggt tcaaaaatat agtcaaaacc agcctgggga
    37261 gttaaatgtt tatccgcctt atcggacagc atttccttgt ttacactcac tttaggatcg
    37321 ccaataacac ctaaatattc attttcagta ttaatgttaa taggcaataa ttgcttgatg
    37381 cggggcactt gcttaaccgg gctaacaaaa tctgtatagg ctaaaaaaac tttatcatac
    37441 cgaccaccaa gaaaatcctt aatcactaaa tttactatcg gcaccacttc cttagagtct
    37501 tctaaaatat cacttttagg aaattcagct gttactgtct gattttgcgc ataaacggcc
    37561 gcgcctttct tgcctactag aataaaatct aatggcactt caggatgacg cttaatggaa
    37621 cctttaactt tattaataat agccgcatta tatccaccac acaggccgcg attagacgtt
    37681 attaaaataa ccgccgcctt tttggtttcc ggcctaagtg tcaacaaagg atgtttttct
    37741 ccgttttgac cattagtggc agacaaatgc aaaacagttt cccaacttaa attagcatag
    37801 gttctggttt ttaaaaccgc ctcgaccgct cttctcattt tagcggtcgc caccatttcc
    37861 atcgctttag taagcttgcg agtattacta atggatttaa ttcgtcttga tatttcttta
    37921 gttttcgcca taaagccaaa tgattacccg cgccttagct taatagataa tcgagggttt
    37981 ccttgtaatc acctaaaagt tgcttaattt tttcttcagt ttcaacatca agctccttct
    38041 tttctaaaat tgccttagag acctcgttgt ttgcctctaa atatttgtat aaaccggatt
    38101 caaaatcacg tactttttct accggcacct tgtctatata accattaatc aaggcataat
    38161 atataaccac ctgcttatca atggacaaag gctgatactg atcctgcttg aatatttcat
    38221 acaaacgctt acctctttcc agttttcggt tggtatcttc atctaaatca gagccaaatt
    38281 gagcaaaagc ggccaattcg cggaactggg cagcctcaag gcgcatcttg ccagccactt
    38341 ttttcatagc cttaatctga gcggaagaac cgacacgaga aacagacaag ccagcattaa
    38401 tagccggacg attaccttta tagaacatat ccggctctaa gaaaatctgt ccatcggtga
    38461 tagaaataac attggtggga atataagcag aaatatcgcc ggcctgggtt tcaataattg
    38521 gcaaagcggt aatggaaccg ccaccaaaat ccttatttaa tttacaagct ctttccaaaa
    38581 gacgggaatg gagataaaaa acatcaccag ggaaagcttc gcgccccggt ggccggcgca
    38641 ataaaagtga aatttcgcga taagaaacag cgtgtttgga taaatcatca taaactatca
    38701 aaacatcttc tcctttatct aaaaaatatt cggccatagc cgtgccggca taaggagcaa
    38761 ggtaaagtaa agaagcgggg ctagaagctc cagctaaaac tatggtggta tattccattg
    38821 cccctgaagc ctctaattta cgaacaattt ctgcaacttt agactctttt tgcccaatag
    38881 ctacataaat acacttcatg ttttgtcctt tctggttgat aatagtgtcg atagcgatag
    38941 cggtcttacc aatctgacga tcaccaataa taagttcgcg ctgtccacga ccaataggaa
    39001 tcatcgcatc aatagccttt atacctgttt gaaccggctg attaacgggg gcgcgggtaa
    39061 tcaccccggg agcaatcttt tcaacaggat aaaactcttt agtagccaaa acgcctttgc
    39121 cgtcaatagc ttcccccaag ggattaacaa ccctgcccac caatacctca ccaactggca
    39181 cgcttaaaat acgcttggtg cagacgactt cgtccccctc cttaatcccg gaaaaatcac
    39241 ccataataat agcgccgact aaattttctt ctaaatttaa agcaagaccg gcaatttcac
    39301 cctgactgga cttaaaaatt aaaatttcgc tcatcattgc ctcagttaaa ccagaaacaa
    39361 gagcgatacc gtcggaaatc ttcaaaactc ggcccacact gctctcttca aggctgacct
    39421 gaaaatcagc aatttgccgc tttatctgct cgacgataaa atctttagtg ctagacataa
    39481 tattaattaa ttaaaagctg tttcttaaat tttaataaat tatttttaaa gctgccatca
    39541 attactagac cattataacg gagaatgaaa ccaccaataa gatctttatc tattttttcg
    39601 gataactcta tatctgtttt acctaacttt gacttaatat aagcgcttat cttagattta
    39661 atttgattgt ccagcggccg agaacttaat agttccgctc tggtcaatcc gtcagaactg
    39721 tcatacagag attctaattc attaataata gccttagcct tatcaaaatc ttgacggcga
    39781 taaagaaatt ttacaaaatt tgcaattaaa ctcttagctt cattctcggt tttaccctct
    39841 aatagctcaa agagactacg agcgtattgt ttggcattca ctttcataaa attaaattaa
    39901 atctttgaca aggcggtcaa tcgcttcttt atcgcgctta tcatcaacat tctcgtgtaa
    39961 aaaacttttt aaacccaata aaattaagtt cgataactcg cctttaagtt ccgctaaaac
    40021 agactttttt tcggcttcaa ttttttctct ttccttctcc atcataatac cgatatcttc
    40081 ttgggctttt ctaacgattt cttgctggcg taactccgcc tgatgttgag ctttttccaa
    40141 taattgcgcc gcttcttggc gagcggcaat catcacctga cgactattct cttcagcttg
    40201 ttttaatttt tcggccgctg tttccgcatc attcaagcct tgttctatct tagagcgtct
    40261 ttcttccaaa atatttaaaa ccggcttata ggcataacgc cataagatga acactaaaag
    40321 agcaaaatta aataattgtg ctaaaagaat gcggccatcg atgccgagag ctttgattaa
    40381 acccatataa aaattttaaa ttaaaacaca aagcaatccg ccgcaaaaag aggcggacag
    40441 gctctgcttt ttaagcaaaa aggataatta aagcaatcac taaggagtaa ataccggtgg
    40501 attcggtaat cgccatcgcc acaaacatta aaggggcgat tttaccatta gcttccggat
    40561 tacgaccgat tgcctc
//
LOCUS       DAOQSA010000004        35955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_618_length_35955_cov_9.576908, whole genome shotgun sequence.
ACCESSION   DAOQSA010000004 DAOQSA010000000
VERSION     DAOQSA010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 35955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35955
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_618_length_35955_cov_9.576908"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..81
                     /locus_tag="PKK65_00885"
     tRNA            7..81
                     /locus_tag="PKK65_00885"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40..42,aa:Pro,seq:cgg)
     gene            208..1725
                     /locus_tag="PKK65_00890"
     CDS             208..1725
                     /locus_tag="PKK65_00890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNU81276.1"
                     /translation="MIDFKQKLEEKDPESRNLHQAEEEEKFINDLKKNRKTKNYFIAI
                     IVLVLIFSGKVIMSSQEAGQWLANNTFWGRVTHLTENNDNQLVGEEQDRINILLLGMG
                     GANHDGGYLADTIILVSLRPSTKQAALLSIPRDLTVPTDNGVWRKVNSINALAEAKEE
                     GSGGPAMTKSLSSVLGTDIYYYISIDFDGFINIIDELGGIDINVENTFDDYSFPIRGE
                     EDNPDYYARFEHLHFDQGWQHMDGALALKYARSRHASGLEGSDFARAKRQQLILEAVK
                     TKLLSRHTLLKPAMLSRIISELNHNVKTNLDVWALIKLWNDYKDIDRSQISNKVLNDG
                     PNGLLMASRGENDAYILIPQTGNFKVIQNFVQNIFDENTNITTQNNQSEATKTPLESE
                     NSATNNPIAISKVQGEAKVAVLNGTWTSGLAAKTAANLQKYGFKINETANAPTREYKN
                     SVIYDLTYGQKLQSLEILQAASQATLAYDAPTWLETYKNIPDKPDFILIIGAKQQ"
     gene            1734..3125
                     /gene="der"
                     /locus_tag="PKK65_00895"
     CDS             1734..3125
                     /gene="der"
                     /locus_tag="PKK65_00895"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNU81277.1"
                     /translation="MTPPKEKLPLVAICGRTNVGKSTLFNRLIEKRQALVSDIEGTTR
                     DCNLGLVHWNRKIFRLVDTAGLLDAGFISGKKKIDNDIDSLAQQQALNYLREAKLLLF
                     VADGKAGLLTEDRLLAKALRQNPLYSAKTILVVNKIDSGKWLTEAVVFNKLGLGEPIT
                     ISAQTGAGTGDLLDIVLKKIEAATDLNEEEEREITAKLCIVGKPNVGKSSLLNALLGY
                     NRVIVSDRPHTTRESQDTELIHKDKLIRLIDTAGISRHGHKSKGLEKEGIAMSLQSLQ
                     RADIALLVLDISEELTHQDAKIVEKIVERQKSLIIIANKWDKVEEKNQKHWQEKIFSK
                     LPFAAWAPLLFVSAKTGAKVNKILDIALEVASARKTEISETQLKKFLSRVVKIHLPAK
                     GRGLKAPHIYEIAQTRHNPPSFTVRIGSRDDLHFSYVRFLENRLREQYDFMGTPIKMS
                     VIKNKKKHGVREA"
     gene            3280..3837
                     /gene="pth"
                     /locus_tag="PKK65_00900"
     CDS             3280..3837
                     /gene="pth"
                     /locus_tag="PKK65_00900"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNU81278.1"
                     /translation="MKIIIGLGNPGDKYALSRHNSGWLALDMLAGADGWRPEKKFNAL
                     IKEKGGIIYLKPLTLMNNSGETAYRVLRYYHLLEKQFGCITKKNQDLRDWLIVIQDDL
                     DLDLGKWKISDDSRSGGHKGVQSIINHLKTQRFIRLRLGIKNGELRRLIPPEKFVLQK
                     FSTEELPILQDSIKKGLAALKPYIP"
     gene            4168..6390
                     /gene="topA"
                     /locus_tag="PKK65_00905"
     CDS             4168..6390
                     /gene="topA"
                     /locus_tag="PKK65_00905"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNU81279.1"
                     /translation="MKLIIVESPTKAKTISKFLGKDYRVESSFGHIRDLPTKEMGIDI
                     EHGFKPKYITPLKAKKTVSALKNATNKATAVILASDEDREGEAIAWHLTQVLKLDADK
                     TERIVFHEITKQAILKALETPRHINQNLVDAQQARRILDRLVGYELSPFLWKKVTKGL
                     SAGRVQSVAIRLIVEKEKEIQAFKSQDYWTLRARLQGEKNKEEFTAGLFRLNGKPFDK
                     LEISQEIALETQKELESAKFKISHIEQKKVRKTPGGPFITSTLQQGANRHLGFSAKQT
                     MMVAQKLYEQGFITYMRTDSLNLSPQFLNEARDYLKNTLGEKYTLPSPRIFKTKSKNA
                     QEAHEAIRPTSVAATPEKLKSKLDYNGYRLYKLIWERSIATQMPAAQLNATVIDTEAL
                     CNNGKQHTLRANGQILIFDGYLRIYPEKTAEQELPKLRENEKVILLSIETEQHATQAP
                     ARYSDASLVKDLEKYGIGRPSTYAPTINTIITRNYVIRNEDKRLQPTDIAMVVNELLV
                     EHFPKIVDYNFTAEMENDLDEVAEGKKKWQPVISKFYQEFHQNLQNKYDSLKKSDIMP
                     EEKSEEICDKCGAPMVIKTGRYGKFLACSAFPNCRNIKKLNGHAAKTEDPALAALQEK
                     YQGTVCDKCGAEMKVRSGKYGPFLACSAYPKCKNIKNINDGTSKEKEIDCPACKTGKI
                     VKKFSKRGIFYACNNYPDCKNTYWGQPTGDSCPHCGSLLIKNKTGQTVCSDKGCGYQA
                     "
     gene            complement(6540..6866)
                     /locus_tag="PKK65_00910"
     CDS             complement(6540..6866)
                     /locus_tag="PKK65_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81280.1"
                     /translation="MFETSRDILNWVLAVSAGALAFFLCWSLYYFIASAQRIFRLIKQ
                     VEQGVEKAEALLDLIKEKVSGSASYLMILGELLKRGIDFAKERSKHRSSEGGDDISAK
                     SKKKKK"
     gene            complement(6919..9342)
                     /gene="pheT"
                     /locus_tag="PKK65_00915"
     CDS             complement(6919..9342)
                     /gene="pheT"
                     /locus_tag="PKK65_00915"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNU81281.1"
                     /translation="MYLSLNWLKDFVKLPKAINPTDIAELLTLHIVEVESWKEQKAVF
                     EKVVVGRVVSVAPHPNADRLRLIKVDIKGEILDIVCGAPNVAFGQKVPVALVGATLPN
                     GVEIKESEIRGEKSYGMICAEDELGLGSDHEGIMVLSDKAKIGQSFADYLNFNDIILE
                     IDNKSLSNRGDLWGHYGMARELSALLKSPLRPYLSNLENKDLHGSGKSQLDVKIEDKS
                     ACFRYIAVKINHVKTIESPRWLKERLVAVGARPINALVDITNYVMLESGQPLHAFDAS
                     GLEKISIRASKDGESLETLDEKERLLPKNSIVITDGYEPIALAGIMGGKRCAVNSDTE
                     SIILEAASFDAVRVRQTSQALNLRTDASMRFEKTLDPNLPEQAWQRAWQLIKEIMPEA
                     ELAGQPVDIVNFKLEPSPIEVDFSWLKKRLGYSLTRKETISILERLGFKVDVSKNILA
                     VIPPSWRAVKDVSIKEDILEEVARIVGYNNILSQSPISPLTPLPDNLELSLERKIQDI
                     LSGAGRLHEVYNYSFVSEAVLSKLNLDPSRCLRLANPISEQHVFLRPTLLAGLWQDAR
                     LNQFNFNQFGIFELGRIFLPAPGEYMKDDRGDFLPYQGKQLAMLFASQDNSVSFNRIK
                     GLVNLLFTKLWPDSRVEFLALENQPAWSQAGQAVAVFCAGKELGVAGLLDNKLANAFG
                     LKLKTAVVELNFKEILELSQSLASNHYRELSKYPPVVRDVAFVVEEKILYNDFWRTLI
                     SFHPLLVKAELFDIYRGEALGSGRKSWAFHLTYQAEDRTLTALEIDAIQTDLVNRCHD
                     QFEAQIRDF"
     gene            complement(9358..10386)
                     /gene="pheS"
                     /locus_tag="PKK65_00920"
     CDS             complement(9358..10386)
                     /gene="pheS"
                     /locus_tag="PKK65_00920"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNU81282.1"
                     /translation="MKNKLEKLRDDFLESLQKINDLPALRELELKYLSRKGELNGLLG
                     ELKDLSLEMRREAGELANVIKQELQERFNEVKTEIEKKEESAAYLDPTVPGEKITTGS
                     LHPLTLLRRELEEFFATLGFMVMDGPELESEFYNFEALNVPPHHPARDMQDTFYVDLK
                     NKQGEYDLTMRTQTSNTQVRAMRKYGVPLKVIMPGRVFRSEATDARHEHTFYQLEGVM
                     VGKNINFAQMKSVLEAVGKKLYGPETKFRMRPKFYPFVEPGSNGEYTCFLCQGKGCHV
                     CKYSGWLEILGCGLIHPDVLRAGGVDPEQYSGFAFGFGLNRLAMLKYGINDIRLFNAG
                     DLRFLQQF"
     gene            complement(10414..10797)
                     /locus_tag="PKK65_00925"
     CDS             complement(10414..10797)
                     /locus_tag="PKK65_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81283.1"
                     /translation="MTLKNYLIVMGVMTAVCWGVFILMLNFIDPTVTNWLGFMLFYVA
                     LFLALSGTAALVGFIIRFVALRRELAFYAVATAFRQSFLFALFIVIALFLLSANLFTW
                     LNLILLLIIFSILELFVASYKKSRL"
     gene            complement(10816..13449)
                     /locus_tag="PKK65_00930"
     CDS             complement(10816..13449)
                     /locus_tag="PKK65_00930"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNU81284.1"
                     /translation="MKKELAKIYEPALFEDNIYRLWEDSGFFNPDNLDLPAGAPSYTI
                     VLPPPNITAKLHLGHSAMLAIEDLMIRYHRQNSYRALWLPGTDHAAIATQNMVEKKIF
                     SETGKTRHDLGRKVFLEEVDKFVAETQATILHQIRKMGASLDWSRLAFTLDSPRQQAV
                     QRMFLDMYNEGVIYRGERIVNWCPRCHSTLADDEVEYKEQSAFLYTFKYDPNFPIAIS
                     TTRPETKLGDTAVAVNPKDERYQQYIGQEYEVNFCGQDLRIKIIADYNVEMDFGTGAL
                     GVTPAHSAVDWQMAEAQSLPKIKVINEDGLIREGFGEFSGLSVIAAREKVVASLRNQG
                     LMIKEEEIVNNLSCCYRCDNPIEPLPSRQWFIAVDKPLARLQGKSLKEAAIAAVREGE
                     IKFLPERFNKRYLDWMENLHDWCISRQIWFGHSIPAWYRGEEIYVGEEAPAAEGWIKD
                     NDTLDTWFSSGMWTFSTLGWPDNFKDGIKTGDLARFHPTQVLETGYEILTLWVSRMVM
                     MSFFALGEKPFSQVYLHGTILDKNGKKMSKSKGNGVDPLEVIAKYGADAVRLSLLMGA
                     TPGNDSRYSEEKIESKRNFINKLWNISRFILNNLDDEILSTPIDVLPEAKSLSDNWIL
                     GELLSTRKTLATSLSDFNFSFAAETLYDFTWNKLADWYLETTKIEKGKETILVFILKN
                     LLIMWHPFIPYVTEAIWQSFNNTLLMVEPWPKLANKLDYWINNPGKEADFSFVQEAII
                     AIRNARSENKVEPARKLQALVTGPKSELLLSQAPLIKGLRTGIESLLATDGPPDGQAI
                     KVVLRDVDIYLLGAVDPEKEKARLRKEKENLLNLISGLRAHLENQEFIDRAPAHIVQA
                     EKDKLARYEIELEKIRASLGV"
     gene            13566..14732
                     /locus_tag="PKK65_00935"
     CDS             13566..14732
                     /locus_tag="PKK65_00935"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNU81285.1"
                     /translation="MLTLEQQIFKQIDKSENILIVFNADWNGDAVASALGLFLYLKKI
                     GKKTEIAAHLAEDDAAANKAAAAWRFLPAFEKIQSSLKNLRKFIVSLDISQATINQIK
                     YTVDGHKLNFIISPEKGWFTPEDISTSSSGFKYDLIFVLDTPDFESLGAIYDNNVEFF
                     YKTTVINIDHQADNEEYGQINYLDLNVVSTAEMVYNLIKQHSQEPLDEDIATCLLAGI
                     ISKTKNYKSANLTPRTLLTSSKLIGLGARREEIINQLYRSKPLAVLKSWGKILNNLKT
                     EDQGRIVWSWLEEKDEISALINGQLDVQEIFEEISASVAEAKFIFIAGVHPQSEGIFI
                     LIQAAKGFSSIEVLKNYSPAGNTRIASAILPFSREEWQEKFIAALKDKLDKNKI"
     gene            14808..14884
                     /locus_tag="PKK65_00940"
     tRNA            14808..14884
                     /locus_tag="PKK65_00940"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14842..14844,aa:Ile,seq:tat)
     gene            14940..15013
                     /locus_tag="PKK65_00945"
     tRNA            14940..15013
                     /locus_tag="PKK65_00945"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14974..14976,aa:Val,seq:gac)
     gene            15031..15447
                     /locus_tag="PKK65_00950"
     CDS             15031..15447
                     /locus_tag="PKK65_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNU81286.1"
                     /translation="MLKFFSGNKHHLVQTEDTSSISDWMEKEAVSEGQLSIDVYQTDR
                     KIIIQSTIAGAKPEDLKVSLHHDLLTIHGTRKQPQMVADEQYLYRECYWGSFSRSIIL
                     PAEVDPRKVEANLENGVLTVSLTKTKPENIAVNFKD"
     gene            15450..15638
                     /locus_tag="PKK65_00955"
     CDS             15450..15638
                     /locus_tag="PKK65_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81287.1"
                     /translation="MNYELTVAGFSDDQVILKDDNNSLIYWPKNKLPQIPEIGQKINF
                     SIGDNRAEILNELLRTDN"
     gene            15649..17487
                     /locus_tag="PKK65_00960"
     CDS             15649..17487
                     /locus_tag="PKK65_00960"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HNU81288.1"
                     /translation="MPKKHLPIIAIIAVLVVFLFLIVLTANLGKSDSTLSDNLYFGGL
                     DISGLDKAQAEIMIKKEYQTMVEKGLTLKYGDSFLSLPATSASFDADLSYPHFHYTDD
                     NIKASLEAHTKPGLLARWWRQLFGPKNIYITPTFKLDEEKIKKTVQTSLTDAFYPAVD
                     AAFVFTKNNIEITPERPGKTIDWEALFKDIKNRLELFTTDPIVVKTKTEYPEIYARDL
                     IGLENEADNLSRNPLTLVFNDKSWPVTREEIASWLYVRKQDGKLNLAFSPERIAKYLN
                     DNIADKINVSPQDPRFEIKDNKVSSWQLGKDGYELDSIASASEIINQYFASSEKNEKN
                     ISLIVKTLPPAIAEDFDIKEIIGTGHSNFAGSSANRRHNIEVGAAAVNGILIKPGEEF
                     SLVKTLGAINASTGYLPELVIKNNKTIPEYGGGLCQIGTTIFRSALASGLPITARQNH
                     SYRVSYYEPAGTDAAVYDPWPDVRFINDTPKAILIQSRIEGNDIYFDFWGTKDGRSVT
                     TTTPVIYNIVKPPATKIVESDELSPGEKKCTEQAHNGADTYFDYTVIYPEGATSTPLE
                     KTRRFSSHYVPWQEVCLVGKIATSTPDTETASSSPTVETGSIEINN"
     gene            complement(17768..18595)
                     /locus_tag="PKK65_00965"
     CDS             complement(17768..18595)
                     /locus_tag="PKK65_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNU81289.1"
                     /translation="MRLFIFIIFVVSVIFSFSNFRPHRAEVVFFNVGQGDSFLLHTPG
                     GLNILIDGGPDWSTLYALGRYFSGHQPVIDLLILSHGHDDHLAALPELVERYQVKRAF
                     LPARLAGYAGSALSSSLSESGTIISYPMENFCFELEIACRLCLFPPGKEFLDSTDDND
                     LSLAINFNCAGLSLTATGDASGRREQALLAENDYLGAHILKASHHGSANANSDDFIKA
                     VNPKAFIISVGVNNYGHPAAELIHRVRADGIQIWRTDEDGDVLFYSKNSQLYTAKPW"
     gene            complement(18997..20385)
                     /locus_tag="PKK65_00970"
     CDS             complement(18997..20385)
                     /locus_tag="PKK65_00970"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNU81290.1"
                     /translation="MSKKDADNSLDSIVALCKRRGFVFPSSEIYDGFTAVYDFGPYGV
                     ELANNIKKEWWRAMTQLRDDIVGLDAAIFMSPKVWQASGHVKGFSDPLTECRQCHSRL
                     RLDTLLEEAGILADEKMSEEEINNIFENNKSKIKCPNCGSSDFTPGKNFNLLVQSNLG
                     NFTGDWSKEPIYLRGETCQGIYVNYKNVLNSTRVKIPFGIAQIGKAFRNEITTRQFIF
                     RTREFEQMEMQYFVKPGDDMKYFEQWRQTRWQFYLDLGIKEKNLQWHKHENLVFYAQE
                     AYDIEYKFPFGFKELEGVHARGDYDLSVHSQSSGIDLDYLDPISNEKYIPHIIETSAG
                     VGRTFLAILTESYREERVGEELRTVLALPSKLAPIKIAIFPLLKNKPELVVRAREIFD
                     FLKLNWRCEFDDNGNIGKRYRRQDEIGTPYCVTVDFDTLEKGEVTVRERDSMQQTKIK
                     VENIKKYFQDKF"
     gene            complement(20427..22133)
                     /gene="argS"
                     /locus_tag="PKK65_00975"
     CDS             complement(20427..22133)
                     /gene="argS"
                     /locus_tag="PKK65_00975"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNU81291.1"
                     /translation="MFLANLKERLASLLSVSADDFVYPVPAELGDLSLPLFALAKSSG
                     LSPAALAQEKIKLLEQSDLPEAIQSFRAVGPYLNFFFKAGVFNREALFDIEKAGPDFG
                     RLAVGRQQKVMLEYSNANTHKEYHIGHLRNLFFGDAVFRILAFNDYRAIPVSYINDFG
                     IHVAKTLWQWQRANYKDVDAPKGWLLGKCYAEASNLLLQAEIKEDGGLEKEKMKTEIS
                     TVMKEIESRQGKNYELWQETRAWSIAYFAEIYQRFGVRFEHIFYESEVIDDGLKMVAE
                     FLQRGILKNNQGAVIADLEEYGLGVLPIIRSDGTALYPVADLALARLKFNEYHLDESV
                     YIVDVRQGLYFKQLAKIFELAGENLKISHLGYDFVTLRGGMMSSRNGNVITYSQIWDE
                     AYTRTRHEVVARHQDWEIKRIESTAEKLAVAALKFEMLKVRADKIITFDMDEALRFDG
                     FTAAYLQYTGARLASILRKNETLTADYSLLIHGKELSLAIMMQRFPEAVAKAGFQRDP
                     SILARYLFDLSQVFNDYYHEVAILKAEPALSSARIGLLRSLRQVLVNGFSLLGFDYLE
                     EM"
     gene            complement(22144..23367)
                     /gene="tyrS"
                     /locus_tag="PKK65_00980"
     CDS             complement(22144..23367)
                     /gene="tyrS"
                     /locus_tag="PKK65_00980"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNU81292.1"
                     /translation="MAKVNTDSNLIAEILERGVEKIYPNRENLEKKLKSGEVLRLYCG
                     FDPSASSLQIGNAIALIKLAQFQRLGHKVIFLVGTFTGMIGDPTDKGSVRKQLSREEV
                     TANAVNFQKQAGAYLNFTGDNPAELRYNHEWHDQLSFKDLLGLASNFTVQQMIQRDMF
                     QKRLEEEKPIFLHEFLYPLAQAYDSIVLDVDVEVGGNDQLFNMMCGRDLLKALKQKDK
                     DVLTLKLLTDSEGKKMGKSEGNIVMLDESPNNMYGQVMSWADDILPSAFELCTFLPWA
                     EVKDIQAGLSSGKLNPRDVKMKLAWEITRINHGEKDADASQDYFIKTVQNKEMPDEVE
                     SKVVPAGNYKIADLLVALNLCSSRGEARRLVDQGGIKVGLVNNMKTLTDSQAEIVVSP
                     GLIIQRGKRQFIKIS"
     gene            23490..24461
                     /locus_tag="PKK65_00985"
     CDS             23490..24461
                     /locus_tag="PKK65_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81293.1"
                     /translation="MFSYRFFLKQAWNITKRYRYLWFFGLFASLIALGGEYQIISQGL
                     NNNPGGSFVSIGVFMLYNLFNPSFYAGIGSLAASNPAALWSLITALLLALALIIIMVY
                     LAIVSQAALVEHSAQIILNKKKKIGLSINEGLASGRKHFWSLLGLNISGNIIITFSFL
                     IISLPLVFLFITDTSVLTLAYILLFVIFVPISLSIAFIIKYAIAARVLENLSFVAAME
                     KGWKIFQNNWLVSLEMSLILFLINFAVGIVTIAVISLFFLPLLLLSLQLYLPLLTALS
                     FILTLAAMLVAASIFNTFQISTWTNLYIHLQEKKGLSKLERIFQRQR"
     gene            24545..27559
                     /locus_tag="PKK65_00990"
     CDS             24545..27559
                     /locus_tag="PKK65_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNU81294.1"
                     /translation="MSDIASIENLIDPDTAKDEDSVQGKFAKKQQEIKLKEIEKQTEQ
                     NAAAIGLPYVNLFGFPISPEALVLIPEERSRELKAVCFFYDGKNIRVACLEPNEEIAL
                     EIQRLNEQYFADTKLYLISQNSLNQALEAYKAVPKVKKYDSGVEISEESLEKFKQDIA
                     SYKNLNEKINEVNVSDIVTLLLATAIKVGSSDIHIEAEENGVIIRFRIDGVLQEAAVI
                     DKEKWKKIITRMKILAKVKINISNKPQDGRYTIYLKDGKIDVRSSFLPTAYGESVVMR
                     LLNSKAVALEFESLGIRPEILEKLKQEISKPNGLILTTGPTGSGKTTTLYAILNILNQ
                     PGTKIVTLEDPVEYQLAGINQSQVDASKGYTFSDGLRSILRQDPNVIMVGEIRDLETA
                     EIAIQASLTGHLVLSTLHTNDAAGVIPRLIDIGVKPYLLVPSINAVISQRLVRKLCPY
                     CRQEHRLSMEEEEKVQKILAVISPRAKVNTPSKLPTIYKAGAGCEQCNYLGYKGRIGI
                     YETFTMDDNLKQLTIDKAPSFKILQQAIENGMITMLQDGVLKVMDGITSLEEVYRVIG
                     NFNYVDELYDIVISQTIGRGVKITAAEYERGQALTKHIGQAINLSDIPSKELIKMIMS
                     MASASDAGDVHIEPEENGVAVRFRIDGVMHDILDLPKTSYLPILSEVKILAGFATNIK
                     RATLDGRFSVYLPEKKFDCRLSIISGGYGETIVIRLLINSAASLDLEKLGITSVAKKE
                     LDKAITKTKGIIIATGPTGSGKTTTLYGILNKLNKSDVKIITIEDPIEYQLPGIMQTQ
                     IDTERGYTFAAAMRSLLRQNPNIMMIGEIRDEETAKIAIEAAMTGHLVLSTIHANSAA
                     GAISRFAGLGVDKSSLANAIEFSIGQRLARRICPHCKIEDTPGQEMIEQAKSILSSIK
                     NKEVVIPKTLKFYKGAGCEQCNHLGYKGRVGLYETIPSSNSIRKLIQKEETTDYDIEQ
                     EAINEGTISIAQDGVLKALAGETSLEEVFRVI"
     gene            27598..28173
                     /locus_tag="PKK65_00995"
     CDS             27598..28173
                     /locus_tag="PKK65_00995"
                     /inference="COORDINATES: protein motif:HMM:NF014099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81295.1"
                     /translation="MINTAFITKLKNDYRQREQERRQIISRSNDVLFQAKKTIFALQR
                     EELSLAEEKLRSMEAVLKKMEKDFSPNRLREEGAYHAAAEEYVEAKTLAAIVSGKKIN
                     AITGLHLGYDSYLGGLCDLIGEMVRLATNRAAKGKYESVAELKDKAEEIMNSLLDFDL
                     TGYLRTKYDQARGHLRKLEQMAYEIRLRKDK"
     gene            28179..28673
                     /locus_tag="PKK65_01000"
     CDS             28179..28673
                     /locus_tag="PKK65_01000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HNU81296.1"
                     /translation="MLIAIAADLHDNLANWQKFKEYSKKQGIDTLLFCGDLCNRDTLK
                     KIATNWKGKIFMIAGNQELYNQAETKTLPSISFLNRYGVIEINNLKIGLIHEPEYEKR
                     ITRENKGLNYLFYGHTHKPWFVRKNDIIIANPGTLGGVFYKASFAVLDTDTGKLELKI
                     LENI"
     gene            complement(28728..31205)
                     /gene="uvrA"
                     /locus_tag="PKK65_01005"
     CDS             complement(28728..31205)
                     /gene="uvrA"
                     /locus_tag="PKK65_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNU81297.1"
                     /translation="MLENNIKIKGARTHNLKDINLDLPRNKLIVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFVGLMDKPDFDLIEGLSPAIAIDQRTAGNNPRSTVGT
                     VTEIYDYLRLLYARVAWPYCPHCQTRLKKAEKSSGRKNKKKQEPSHYVYACPTCSFTL
                     PGLEPRNFSFNSNYGACPHCGGLGTRTEIDPELVFSLKLNLLQGAIKPLAHANLNTSS
                     ALFVELKKLGERYGFFLETPLQEMSVGQRAAILNGDKQWRGLNAELLERYEKTKSNFV
                     KQEIEKCMRLAVCPACGGFRLKPEYLAAKIKNLSIADIADKTIDILRPLLSKWLLSSS
                     WNGNQAVIHPILKEIIKNLEFLERVGLSYLTLSRPANSLSGGEAQRIRLAAQVGSTLS
                     GVLYVLDEPSIGLHQRDNDKLIAMLKDLRDNGNTVIVVEHDAAMMRAADYLVDVGPGA
                     GEHGGKILFSGKPEDIKDCRQSLTGAYLSGVKSIAKRESFRPGNGKFLTVAGASEHNL
                     KNIDVHIPLGKFVAITGVSGSGKSTLMSDILANALNKKFYRAKVDVGAHKEIRGLDNI
                     DKVIDINQSPIGRTPRSNPATYTGLYTYIRDLFAELPEARLKRLTSGHFSFNVAGGRC
                     ETCAGDGAIKVEMQFLNDVYLTCDVCHGQRFQQKILDIKYKGKNIHEVLEMTVSEAFE
                     FFKDNSALRQKLGTLEEVGLGYIHLGQSATTFSGGEAQRIKLATELSRRATGRTLYIL
                     DEPTTGLHFADIDRLLKVLNMLVDQGNTVLIIEHNLDVIKSVDWIIDLGPEGGAQGGY
                     IVAAGTPVEVMKIKESVTGRYLRDVYL"
     gene            complement(31298..32335)
                     /locus_tag="PKK65_01010"
     CDS             complement(31298..32335)
                     /locus_tag="PKK65_01010"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HNU81298.1"
                     /translation="MRYKFYTTSQKAWDGILAALVKAEKSIYIEMYIFLDDTRQTHDF
                     LGKLKEKAQAGLKVVVIVDAYGSISLKSVSVSELRNAGVEFIFFSRWFKRIHRKLVII
                     DEKIAFLGGVNIEDKIRYWYDLQICLRGRVVRPLLRSFAYTYKRCGGKDERILIHSRL
                     PLVKKIKSWVIDTWEIGEKKYSFNNYYRRKLMEAQTSIKIVTPYLMPPRWLLASLDNA
                     IHRGVSVEIIIPQDTDIKFLNKINYANASYLSSVGVIFYLTQAMNHAKVMIIDDKEAL
                     VGSQNLDILSFNFNLELGVFFKQKQAVADLLKIWRRWRDSAVKINLSSQRLVWHHRWL
                     MVILRIFFPFF"
     gene            complement(32418..33218)
                     /locus_tag="PKK65_01015"
     CDS             complement(32418..33218)
                     /locus_tag="PKK65_01015"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNU81299.1"
                     /translation="MKSRPKIGLALGSGGFRGFAHIGVIQVLQENNIPLDYISGASIG
                     ALIGAYFSIYSSFDLLKQKIVMNKKGKLPTLFDLGFRGGLVSGRKFEIFLERVLGRKN
                     FSDCRLPLKIVATDLISGQACILDQGRLATAVRASTSVPLVFEPVRIHGHLLVDGGLS
                     NPVPVNLVREMGADKIIAVNLYHHHEFIHRRFNLPTVALRSTRIAVHNLAKHSLFGAD
                     LVMNPDTSAFMQTNEYKKYFDPPVAKALIDIGRSEAKRFLPEIKRLLS"
     gene            33533..33793
                     /locus_tag="PKK65_01020"
     CDS             33533..33793
                     /locus_tag="PKK65_01020"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNU81300.1"
                     /translation="MKAEKNYYTVKELADIMGLSRITIFKRIKAGKIKAEKIGRNYII
                     YKNDLKEMFSNNLTKDDKIIIKNALKIVLNEYGDTIRMLGKE"
     gene            33797..34231
                     /locus_tag="PKK65_01025"
     CDS             33797..34231
                     /locus_tag="PKK65_01025"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HNU81301.1"
                     /translation="MLKPLTINEIKYLAHRLAIDTMGWDEPIPKFETRYPNVLESCIA
                     TPFQKFERKNLYAGIDGKGAILFYLLIKNHPFQNGNKRLAVASLLVFLYRNNKWIEVD
                     NKELYNLAVWVAQSPSLAKDEMIIFISKFIKKYLRNFKLSEK"
     gene            34319..34555
                     /locus_tag="PKK65_01030"
     CDS             34319..34555
                     /locus_tag="PKK65_01030"
                     /inference="COORDINATES: protein motif:HMM:NF018553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp15 family bacteriophage protein"
                     /protein_id="HNU81302.1"
                     /translation="MDFYHTLNYLNYIKIINMLQNNNISDKNKVKLINNIYNKALSDI
                     NNIKKERDDKIIKLLKNIDDRHIDKILKDIKNTK"
     gene            34563..35846
                     /locus_tag="PKK65_01035"
     CDS             34563..35846
                     /locus_tag="PKK65_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81303.1"
                     /translation="MDFENKQNKIEEFAPEKKPENEKNLEIYDVINNGEKRLSGASEK
                     ISNENIQAISHVENYPGTESRDIENTQGIITETNQKINQVIENTQKNVREIVGKNSET
                     NQEKDLNQEKRPSQDDLREVNIIKRIDNKFSGRENRDEFLNISRKITEKIKGLPDNKK
                     EVYWQKMLSLVKSEQNNDIYYYKELHDEALNELKMVEKNSSLKNRDMAIEDRITKCSS
                     FEDLDVVLSSSEGLSGSSNNYDSENLKNIISDVKNFKLPLNALTRSAGLRDKVKNLLA
                     TPDPLVTTNRFEVTTADGTNIWESQNTPNPELIRLNRGRHWELISKSEYKEILHKAKE
                     DEIGAYVDLLDKYKKENLGEKRNSMINFINNEFIFTKNHLGEIKENLSILYEKTNTPI
                     YKKLLDYINSSENKFDGNQESPFLKKIKSLKEKSN"
BASE COUNT        11992 a         7698 c         6502 g         9763 t
ORIGIN      
        1 ccaccacggg ctgtggtata aaggctatta tacacgcttc gggtgcgtgt gatcccggtt
       61 caattccggg cagcccgacc aaatgaaaaa acgttcaaaa aacgtttttt tataataaat
      121 ttgctaaaaa actaaaatat cattataata caaaagtaaa aaatgactgg tattcaaggc
      181 ctaatggtcg ttggattttt tatatttatg atagatttta agcaaaaatt agaagaaaaa
      241 gaccctgaaa gccgtaatct tcatcaggcc gaagaagaag aaaagtttat taatgatttg
      301 aagaaaaacc gtaaaactaa aaactacttt atcgctataa tcgtattagt tttgatattt
      361 tctggcaaag taatcatgtc cagccaagaa gcgggacaat ggctcgctaa caatactttt
      421 tgggggcgag ttactcatct taccgaaaat aatgacaacc aattagtggg agaggaacag
      481 gaccgaataa atattctgct tttaggaatg ggtggggcta atcatgatgg tggttatctg
      541 gctgatacta tcatactagt cagcttacgt ccttcaacta agcaagcagc cctgctctca
      601 atcccccgcg atttgacggt gccaacagat aacggtgttt ggcgtaaagt taattctatc
      661 aacgctttag cggaggctaa agaggaaggt agtggtggtc cggctatgac caaatctttg
      721 agttccgtgc tcggcaccga catttattat tatattagta tcgatttcga cggttttatt
      781 aatattatcg acgaactagg aggcattgat attaatgttg aaaatacctt tgacgactat
      841 agcttcccca ttagaggaga agaagacaac cctgattatt atgcccgatt tgaacattta
      901 cattttgatc aaggttggca acatatggac ggagcgctgg ccctaaaata tgcgcgtagc
      961 cgccatgcta gcggccttga gggttccgac tttgcccgcg caaaaagaca acaattgatt
     1021 ttagaagcgg taaaaactaa attattgtca cgccacactt tattaaaacc ggcaatgttg
     1081 agccgcatta tttccgaatt aaatcacaat gtcaaaacca atcttgatgt ttgggcttta
     1141 ataaaattat ggaatgacta taaagatatc gaccgtagtc aaatcagtaa taaagtctta
     1201 aatgacggac caaatggttt actgatggcc agtcgcggtg aaaacgatgc ctatattctc
     1261 ataccacaga ccggaaattt caaggtaata cagaattttg tccaaaatat atttgatgaa
     1321 aataccaata taaccactca aaataaccaa tcagaggcca ccaaaacccc gttagagtca
     1381 gaaaattccg ctaccaataa tccaattgct attagcaagg ttcaaggcga agcaaaggtg
     1441 gctgttctga atggcacttg gactagtggc ttagccgcta aaacagctgc aaatctacaa
     1501 aaatacggct ttaaaattaa cgaaaccgcc aatgctccca ctagagaata caaaaactct
     1561 gtaatctatg atttaactta cggccaaaaa ctccaaagct tagaaatcct ccaagccgct
     1621 agccaagcga ctttagccta tgacgcccct acatggctgg aaacatacaa aaatataccg
     1681 gataaaccag attttatttt aataatcgga gctaaacagc aataaataat aagatgacac
     1741 caccaaaaga aaaactacct ttagtggcaa tttgcgggcg taccaatgtt ggcaaatcta
     1801 cacttttcaa ccgtctgata gaaaaacggc aggctttagt ttctgatatt gaaggaacga
     1861 cgcgcgactg caatctaggt ttagtccatt ggaacagaaa aatttttcgt ttggttgaca
     1921 cggccggatt gcttgatgcc ggttttattt ccggcaagaa aaaaatcgac aatgatattg
     1981 attccttggc gcaacaacaa gccctgaact atcttcggga agcaaaacta ctgttgtttg
     2041 tagccgatgg caaggccggc ctcctaactg aagatcgcct actggccaaa gcccttcgtc
     2101 aaaacccttt atactctgcc aaaactatct tagtggtcaa taaaattgac agcggtaaat
     2161 ggctaacgga agcggtagtc tttaataaac tgggtttagg tgaaccaata acaatctctg
     2221 cccaaacagg cgccggaacc ggtgaccttt tagatattgt tcttaaaaaa atagaagcgg
     2281 cgacagacct taatgaagaa gaagaacgag aaattactgc caagctatgt attgttggaa
     2341 aacctaatgt tggtaaatca tcattattga atgccctttt gggctataac cgcgtcattg
     2401 tcagcgatcg gccacacacc accagagaat cacaagacac agagttaata cataaagata
     2461 aattgatacg ccttatcgac actgctggca tctcccggca cgggcacaaa agcaaaggct
     2521 tagaaaaaga aggcattgct atgtctttac aatctctgca aagagccgat attgcacttt
     2581 tggtattaga tatcagtgaa gagctgaccc atcaagatgc taaaatagta gaaaaaatag
     2641 tggaacgcca aaaaagcttg attataattg ccaacaaatg ggataaagtc gaagaaaaaa
     2701 atcagaaaca ttggcaagaa aaaattttta gcaaattgcc ttttgccgct tgggcgccct
     2761 tgctttttgt ttctgctaaa accggggcta aggttaataa aattttagat attgctctag
     2821 aagtagcgtc tgcccgaaaa accgaaatca gcgaaactca actcaaaaaa ttcttgagtc
     2881 gcgtggttaa gatacacctg cccgctaaag gcagaggtct taaagcgcca catatctacg
     2941 aaattgcaca aacaagacat aacccgccct cttttaccgt tagaatcggc tcccgagacg
     3001 acttacactt ttcctatgtg cgctttttgg aaaatcgact gcgcgagcaa tatgatttta
     3061 tgggcacgcc cataaaaatg agtgtcatta aaaataagaa aaaacacggt gttcgagaag
     3121 cctaaagaaa taaccgccaa ccggcggtta tttttatttt atcgtcaatt caaacaaaat
     3181 acgttataat aaaaaataag aatgcctatt ttcacctcct ctccagttac actttatctt
     3241 tctagtttaa cacttttata tatattaatt tgtagcatta tgaaaataat tatcggacta
     3301 ggtaatcctg gcgacaaata tgctctcagt cgacataact ccggctggct ggctttagac
     3361 atgcttgccg gcgctgacgg ctggcgccca gaaaaaaaat ttaacgcctt aattaaagaa
     3421 aagggaggaa ttatttattt aaaaccacta accctcatga acaactccgg agaaacggct
     3481 tatagggtcc tgcggtatta tcatctcctg gaaaaacaat ttggctgtat caccaaaaaa
     3541 aatcaagatt tgcgcgattg gctgatagtt attcaagacg accttgatct tgatttaggt
     3601 aaatggaaaa tcagtgatga ttcccgtagc ggtggccata aaggcgtaca atcaatcatt
     3661 aatcatttaa aaacacaacg ttttattcgt ctacgcttag gtattaaaaa tggagaatta
     3721 cgccgtctta ttcctcctga aaagtttgtt ttacagaaat ttagcaccga agaactaccg
     3781 attttacagg atagtataaa aaaaggttta gcggcgctaa aaccttatat accttaaaac
     3841 aaataaaatc cccttgactg cttatacaaa aagcttagaa agcatccacc gctaggagga
     3901 aagcggctag acaccaaaaa cttttgtaca agcaatcaag ggaattttaa cattccagct
     3961 tagactaaaa aaagccttat gtcaaggcca cacatcacag cgttttaact cttaatatca
     4021 ctaaattaaa ggctgaactc agcttgacac ctcgtcaata aatagtacac tgagtatatc
     4081 ggccatacaa attacaattg ataaaatcac caattttaat atttttacct ataaatagct
     4141 gataattaat aataaaaaat gggaaatatg aagctaataa tcgtagagtc accgactaag
     4201 gcgaagacaa ttagtaaatt tttaggcaag gattatcgag tcgaatcttc ctttggtcat
     4261 atccgcgatt taccaaccaa agaaatggga atcgacattg aacacggctt taaaccaaaa
     4321 tatattactc cactcaaagc taaaaagacg gtgagcgctc ttaaaaatgc caccaacaaa
     4381 gccaccgctg ttattttagc gtctgatgaa gaccgcgaag gagaagcgat tgcctggcac
     4441 ttgactcaag ttctaaaact cgacgccgat aaaacagaac gcattgtttt tcatgaaata
     4501 accaaacagg caattttaaa agcgctggaa acaccgaggc atatcaatca aaatttggtc
     4561 gatgcccagc aagccaggcg tattcttgac cgtcttgtcg gctatgagct ctcccccttt
     4621 ttgtggaaaa aggtgactaa aggattatct gccggacgag ttcaaagcgt cgctatccgc
     4681 ctaattgtag aaaaagaaaa agaaatccaa gcctttaaat ctcaagacta ttggacgctg
     4741 agggctagat tgcagggaga aaaaaataag gaagaattta ccgccggcct cttccgctta
     4801 aacggcaagc cttttgataa attggagata agtcaggaga tcgcgctaga gactcaaaaa
     4861 gaattagaaa gtgccaagtt taaaatcagc cacatagaac aaaaaaaggt aaggaaaacc
     4921 ccaggaggac cgtttattac ttccactctg cagcaggggg ccaaccgcca tcttggcttt
     4981 agtgccaaac aaaccatgat ggttgcgcaa aaattatacg aacagggctt tatcacttac
     5041 atgcggaccg attccttgaa tctctctccc caatttttaa acgaggcgcg tgattacctc
     5101 aaaaacacac tcggagaaaa gtatactctt ccttctcctc gaatctttaa aaccaaaagt
     5161 aaaaatgccc aagaagccca cgaagcaatt agaccgactt cagtggcggc gacgccggaa
     5221 aagctaaaat ctaaactcga ctataacggc tatcgactat ataaacttat ttgggaaaga
     5281 agcattgcca cccagatgcc ggccgcccaa ctaaacgcca ccgtgataga cactgaagct
     5341 ctttgcaata acggcaaaca gcacaccctc cgcgctaacg gacagatact tatattcgat
     5401 ggctatttac gaatatatcc agaaaaaacc gctgaacaag aactaccaaa acttagagaa
     5461 aatgaaaaag tgattctctt atccatagaa actgaacaac acgccaccca agcgcctgct
     5521 cgctacagcg acgccagcct agttaaagat ttagaaaaat atggtattgg gcgaccatcc
     5581 acttacgcgc caacaatcaa taccattatt acgagaaatt atgtcatccg taatgaagat
     5641 aaacgcctgc aaccgaccga tatcgcgatg gtggtcaacg aactattagt tgaacatttt
     5701 cctaaaatag tggattataa ttttacagcg gaaatggaaa atgatttaga cgaagtggct
     5761 gaaggcaaaa aaaaatggca accagttatt tctaaattct accaagaatt ccatcaaaat
     5821 ctacaaaata aatatgatag tctaaaaaaa agcgatatta tgcctgaaga aaaatctgaa
     5881 gaaatatgcg ataaatgcgg agcgccgatg gttatcaaaa ctggacgcta cggtaaattt
     5941 ctagcttgca gcgccttccc taattgtcgc aatatcaaaa aacttaacgg ccacgcagct
     6001 aaaaccgaag atccagcttt agccgccttg caggaaaaat atcagggcac ggtctgcgat
     6061 aaatgcggag cggaaatgaa agttcgttcc ggcaaatacg gcccatttct agcttgtagc
     6121 gcctatccta agtgtaaaaa tatcaagaat ataaatgacg gcaccagcaa agaaaaagaa
     6181 attgattgcc cagcttgtaa aacaggaaaa atcgttaaaa aatttagcaa acgcggaatt
     6241 ttttacgctt gcaataatta tcccgattgt aaaaatacct attggggtca accaaccggc
     6301 gatagctgtc cccactgtgg ctctcttctt ataaaaaaca agactggcca aactgtctgc
     6361 agtgacaaag gctgcggtta tcaagcataa tttataacta aaaccaataa aaaacagctg
     6421 tttatcccag cggtttttta ttaaaagata aggaaaccct gtacgaaaaa gccgtcccta
     6481 agaagggggg ctttttgagc gcggggagaa gattattata ccaaaatttt ctcaatcaac
     6541 tacttctttt tcttcttaga ttttgcagaa atgtcatcac ccccttcaga agaacgatgt
     6601 ttacttcttt ctttagcaaa gtcaatcccc ctctttagca actcccccag aatcatcaaa
     6661 taagaagcgc tgccactcac tttttcctta attaaatcaa ggagcgcctc ggctttttct
     6721 accccctgtt cgacttgctt gataagacga aaaattcttt gcgcgctggc aataaaataa
     6781 tacaaagacc agcacaaaaa gaaggctagg gctccagcgg aaacggctaa aacccaattt
     6841 aaaatgtcgc gtgaagtttc aaacataaat ttctgccata aagaaggcga gatccctcct
     6901 atatgcttga attaaaaatt aaaaatctct aatttgcgct tcaaattgat catggcaacg
     6961 atttactaaa tcagtttgaa tagcatctat ttctaaagcg gttaaagtgc ggtcctccgc
     7021 ttgataagta agatggaagg cccagctttt tcggccgctt cccaaagcct cgccccgata
     7081 aatatcaaac agctccgcct taaccaataa aggatgaaaa gaaatcaaag ttctccagaa
     7141 atcattatat aatatttttt cttcaacaac aaaggcaaca tcgcggacaa ccggaggata
     7201 tttagaaagt tcccgataat gatttgaggc taaagactga gacaactcaa gtatttcttt
     7261 gaaattaagc tcgaccacag cagtttttaa ttttaagcca aaggcgtttg ccaacttatt
     7321 atccaaaagt ccggcaacgc ccagttcttt gccggcgcaa aaaacagcca ctgcttgacc
     7381 ggcttgagac caagccggct ggttctctag agctaaaaat tccactcgag aatcaggcca
     7441 tagttttgta aacaagagat taactaaacc cttaattcga ttaaaggaaa cagagttgtc
     7501 ttggctggcg aacagcattg ctaactgttt gccttgataa ggcaaaaaat cgccgcgatc
     7561 atctttcata tattcacccg gcgccggcaa aaaaatccga cctaattcaa aaattccaaa
     7621 ttgattaaaa ttaaattgat ttaaacgggc gtcttgccag agtccggcta aaagagtggg
     7681 gcgcaaaaaa acatgctgct cggaaatagg attagccaaa cgcagacaac gactggggtc
     7741 aagattaagc ttggataaca ccgcctcact gacaaaagaa taattataca cttcgtgtaa
     7801 acgcccggcg ccgcttaaaa tatcctgaat ttttctttct aaggataatt cgagattatc
     7861 cggcaggggt gttagaggag aaatcggact ctgggataaa atgttattat aaccgacaat
     7921 tctagcgacc tcttctaaaa tatcttcttt gatagaaaca tctttaaccg cccgccaact
     7981 gggaggaata acggctaaaa tatttttaga aacatctacc ttaaaaccaa gcctctctaa
     8041 aatgctgatt gtttcttttc tggtaagact gtaccccaat cttttcttaa gccaagaaaa
     8101 atctacttct ataggcgacg gttctaattt aaaattgacg atatctaccg gttggccagc
     8161 taattccgct tccggcataa tttcttttat cagctgccaa gccctctgcc aggcctgctc
     8221 cggtaaatta ggatctaatg ttttttcaaa gcgcatagaa gcatctgtac gcaggtttaa
     8281 agcttgagac gtttgacgaa cacgcacagc gtcaaaagag gccgcctcta aaataatgct
     8341 ttcggtgtcg gagttaaccg cacagcgctt acctcccata ataccagcca aagctatcgg
     8401 ttcataacca tcggtaataa caatactatt cttcggcaga agccgctctt tttcgtctaa
     8461 agtttccaga ctctcgccat ccttagaggc gcggatgcta attttttcca aaccggaagc
     8521 gtcaaaagca tgcagcggtt gacccgattc caacatgacg taattagtaa tatcgactaa
     8581 ggcgttaatt ggccgcgccc ccaccgccac cagacgctct ttaagccagc gaggagattc
     8641 tatcgttttt acgtgattta tcttcacggc aatatagcgg aaacaagcag acttgtcctc
     8701 tatcttcaca tctaattgac ttttaccact accatgtaaa tctttattct ctaaattaga
     8761 taaatagggg cgaagagggg attttaacaa agcactcagc tccctggcca taccatagtg
     8821 tccccataaa tcaccgcgat tagataaaga cttattatca atttctaaaa taatatcgtt
     8881 aaaatttaaa taatcggcaa atgactgccc gattttagcc ttatccgata agaccataat
     8941 gccttcatgg tcggagccta aacctaattc atcttcagca caaatcatgc catatgactt
     9001 ttctcctcta atttctgatt cttttatttc caccccgttg ggcagcgtag cgccgacaag
     9061 cgccacggga actttttgac cgaaagcgac attgggagca ccgcaaacga tatccaaaat
     9121 ctcacccttg atatcaactt taatcaggcg taaacggtcg gcattaggat gtggagcaac
     9181 tgataccacc ctaccgacaa cgactttttc aaaaaccgct ttctgttctt tccaagattc
     9241 cacctcgact atgtgtaaag tcaataattc agcaatgtcc gtaggattaa ttgctttggg
     9301 tagttttacg aaatctttaa gccaattaag agataaatac ataaatattg aagctgttta
     9361 aaattgctgt aaaaatctta aatctccggc attaaacaaa cgaatatcat taataccgta
     9421 tttaagcatc gctaaacgat tcagaccaaa accgaacgca aaaccggaat actgttccgg
     9481 gtcaacgcct cccgctcgta aaacgtcagg atgaattaaa ccgcaaccaa gtatttctag
     9541 ccagcctgag tatttgcaga catggcagcc cttaccctga cacaagaaac aagtatattc
     9601 tccattggag ccgggctcaa cgaaaggata gaatttcgga cgcatcctaa attttgtttc
     9661 cggaccataa agtttttttc caaccgcttc aagcacgctc ttcatttggg caaaattaat
     9721 atttttaccg accatcaccc cttccaactg ataaaaagtg tgctcgtgac gagcgtcggt
     9781 cgcttcgctg cggaaaacac gacctggcat aataactttt aacggaacgc catacttgcg
     9841 catagctcta acctgagtgt tggaagtttg tgtgcgcatc gtcaaatcat attcaccctg
     9901 cttatttttc aaatcaacat aaaacgtatc ctgcatatct ctagccggat gatgcggcgg
     9961 cacattcaaa gcttcaaaat tataaaattc actttctaat tccggtccgt ccataaccat
    10021 aaaaccaaga gtagcaaaaa attcctctaa ttcccgccgt aataaagtaa gggggtgaag
    10081 actaccggta gtaattttct ctccgggaac agtcggatct aaataagcgg ccgattcctc
    10141 ttttttttca atttctgttt taacttcatt aaaacgttcc tgtaattcct gcttgattac
    10201 attcgctagt tcacccgctt cacgtcgcat ttccaaactt aaatctttca attcccccaa
    10261 aagaccgttc aactccccct tgcgtgacaa atacttaagc tctaactccc ttaaagccgg
    10321 cagatcatta attttttgca aactctctaa aaaatcgtcc cttaattttt ctaatttatt
    10381 tttcatatac tagctatatt ccttatccgt catttaaagg cggctttttt tataactggc
    10441 aacaaataat tctagaattg aaaagataat caataataaa attaaattta accaagtaaa
    10501 aagattagct gacaataaaa acaaggcgat aactataaat aaagcaaaaa ggaaagactg
    10561 ccgaaaagcg gtagcaactg cataaaatgc taattcgcgg cgcaaagcca caaaacggat
    10621 aataaaacca actaaagccg ctgtgcctga aagtgccaaa aataaagcta cataaaacaa
    10681 catgaagcca agccaattag tgacagtggg gtcaataaaa ttaagcatca aaataaacac
    10741 cccccagcaa acggcggtca tcacccccat aacgattaaa taattcttta aagtcataaa
    10801 tttaatatta aagacttaaa cccctaaaga agccctgatt ttctccaatt caatctcata
    10861 acgagccagc ttgtcttttt ccgcttggac aatatgagcc ggcgctctgt cgataaactc
    10921 ttgattttcc aaatgagctc gcaggccgct gattaaattt agaagatttt ctttttcttt
    10981 ccgcaaacgc gccttttctt tctccggatc taccgctccc aaaagataaa tgtccacatc
    11041 tcttaatacc acttttatgg cctgaccatc aggcggtccg tcagttgcca gtaaagattc
    11101 gatgccagtt ctcaggccct tgattaaagg cgcctgggac aataataact cggacttagg
    11161 gccggtaact agagcctgta atttacgtgc cggctcaact ttattttcac tgcgagcgtt
    11221 gcggatggca ataatcgcct cttggacaaa agaaaaatct gcttctttcc caggattatt
    11281 tatccaataa tcgagcttgt tcgctaattt cggccacggc tctaccatta ataaagtgtt
    11341 gttaaaactt tgccaaatag cttccgtgac gtaaggaatg aaaggatgcc acattattaa
    11401 caaattcttt aagataaata ccaaaatagt ttccttgccc ttttctatct tagtcgtttc
    11461 caaataccaa tccgctaatt tgttccaagt gaaatcatag agagtttcgg cggcaaaaga
    11521 aaaattaaag tcgcttaaag aagtagccaa ggtcttacgc gtgctcagca gttctcctaa
    11581 aatccaatta tcggacaaac ttttggcctc gggtaaaaca tcaattgggg tacttaaaat
    11641 ttcatcatca agattattta aaataaagcg ggaaatattc cacaatttat tgataaaatt
    11701 tctctttgat tctattttct cctcgctata acgagaatcg ttgccgggag tcgctcccat
    11761 cagcagagat aagcgcaccg catcggctcc atatttagca attacctcta agggatcgac
    11821 gccattacct ttcgatttgg acatcttctt gccgttcttg tctaaaatcg tgccatgcag
    11881 ataaacctga gaaaacggtt tctcgcctaa agcgaaaaaa gacatcatca ccatgcgtga
    11941 tacccataaa gtcaagattt catagcctgt ctccaagact tgcgtaggat gaaaacgagc
    12001 caaatcaccg gtttttatgc cgtctttgaa attatctggc cagcctagag tggaaaaggt
    12061 ccacatcccc gaagaaaacc aagtatctaa agtatcatta tctttaatcc agccctcagc
    12121 cgctggcgct tcttcgccaa cataaatttc ttcaccgcga taccaagcgg gaatactatg
    12181 accgaaccaa atttgtcgag aaatacacca atcatgcaaa ttttccatcc aatcaagata
    12241 gcgtttatta aatctttccg gtaaaaattt aatttcacct tcacggacgg cggcaatggc
    12301 cgcttctttc agagacttac cttgtaggcg ggccagaggc ttgtcgacgg caataaacca
    12361 ttgcctggaa ggcaaaggct caatcggatt atcacagcga taacaacaag aaagattatt
    12421 aactatttct tcctccttta tcattaagcc ctgattacgc aaagaggcca ctactttttc
    12481 tcgagcggcg attacgctta aaccgctgaa ttcgccaaaa ccttcacgga taagaccatc
    12541 ttcattaata actttaattt ttggtaatga ttgtgcctcc gccatctgcc aatcaacggc
    12601 tgaatgagct ggggttacac ctaacgctcc agtgccaaaa tccatctcga cattataatc
    12661 agcaataatc ttaattctta aatcttggcc gcaaaaatta acttcatatt cttgacctat
    12721 atattgttgg taacgctcat ctttgggatt aacggccacg gcggtatcgc ctaatttagt
    12781 ttccggacgt gtagtggaaa tagctatagg aaaattaggg tcatacttaa aagtataaag
    12841 aaaagcgctc tgctctttat attcaacttc atcgtccgct aaagttgaat ggcagcgcgg
    12901 gcaccaatta actatacgct caccgcgata aataacgccc tcattataca tatcaagaaa
    12961 catcctctgc accgcctgct gtcggggaga atcaagggta aaagctagac gagaccagtc
    13021 caaagaggcg cccatttttc tgatttgatg taaaatcgtg gcttgagttt cagctacaaa
    13081 tttatcaact tcttccaaaa aaacctttcg ccctaaatca tgcctggtct ttccggtttc
    13141 agaaaaaatc tttttctcca ccatattctg agtagcgata gcggcgtggt cggttccagg
    13201 aagccagagg gcacgatagg aattctgacg gtgataacga atcattaagt cttcaatcgc
    13261 caacatggca ctatggccaa gatgtaattt agcggtaata ttaggaggag gaagtacaat
    13321 cgtatagcta ggggcgcccg cgggtaaatc aagattatcc gggttaaaaa aaccggaatc
    13381 ttcccaaaga cgataaatat tgtcttcaaa taaagccggc tcgtaaattt tggctaattc
    13441 ctttttcata ccttttaaat taccaaaaat atgccattgc gtcaaccata aattgccttg
    13501 actctagcaa aaaataatta tatattaaat ggtcggacta ataaaaaact aaggaaaata
    13561 aggggatgct tactttagag caacaaatct tcaagcaaat agacaagtcg gaaaacattc
    13621 tgattgtctt taatgctgac tggaacggcg atgcggtcgc ctcggctcta ggtttatttt
    13681 tatatttgaa aaaaatcggc aaaaaaacgg agatagccgc ccatttggcg gaggatgacg
    13741 cagccgccaa caaggccgct gccgcttggc gctttctgcc tgcttttgaa aaaattcaaa
    13801 gttcgcttaa aaatttacgc aaatttattg tgtctttaga tattagccag gccactatca
    13861 accaaataaa atatacggtc gacggccaca agcttaactt catcatttcg ccggaaaagg
    13921 gatggttcac ccccgaagat attagcactt cttccagcgg ctttaaatat gacttaatct
    13981 ttgtgttgga tactcccgac tttgaatctt tgggagcaat ttatgacaat aatgtagaat
    14041 ttttttataa aacaaccgtt attaatatcg accaccaagc ggacaacgag gaatatggcc
    14101 aaattaatta tctcgatctt aatgttgtct ctacggcaga aatggtatat aacctcatta
    14161 aacaacattc ccaagaaccg cttgatgaag acattgccac ctgtctgctt gcgggaatta
    14221 tcagcaaaac caaaaattat aaaagtgcta atctgacacc gcgcaccctg ctaacaagct
    14281 caaagcttat cggtttgggc gcaagaagag aagaaataat taaccagcta tatcgttcta
    14341 aacccttagc ggtcttgaaa tcttggggta aaattttaaa taacctaaaa acggaagatc
    14401 aaggaaggat tgtttggtcc tggctggaag aaaaagatga aatctccgcc cttattaacg
    14461 gacaattgga tgtccaggaa atatttgaag aaataagcgc cagtgtcgct gaggcaaaat
    14521 ttattttcat agccggcgtc catcctcaat cagaaggcat attcatatta atacaggccg
    14581 ccaaaggctt ctcgtctata gaggtgctaa aaaattattc ccccgccggc aatacccgca
    14641 tcgccagcgc catccttcct ttcagtcggg aagaatggca agaaaaattc atcgccgccc
    14701 taaaagacaa acttgataaa aacaaaatct aatgatataa aggagaagaa ttaattcgaa
    14761 attaaacgat taccggattt aattgcgaat ccttgaatca taaatacgcg cctatagctc
    14821 agtcggttag agcacagctc ttataaagct ggggtcgatg gttcaagtcc atctaggcgc
    14881 accagcacca tccgttaata ttgccaagtt ctcacctata caatataata agaaaataag
    14941 ggctcgtagc tcagttggct agagcgttac gttgacatcg taaaggtcac ccgttcgagt
    15001 cgggtcgggc ccacattatt tttcttatat atgttaaaat ttttttctgg caataaacat
    15061 catttagtcc aaactgagga tacctcaagt attagcgact ggatggaaaa ggaagccgtt
    15121 tccgaaggcc aactttcaat cgatgtttat cagactgaca gaaaaatcat cattcaatcg
    15181 actattgccg gcgctaagcc ggaagattta aaagtatctc ttcatcatga tttactgact
    15241 atccacggaa ccagaaaaca gccccagatg gtagccgatg aacaatatct ctatcgtgaa
    15301 tgctattggg ggtcattttc gcgctcgatt attttgccag ccgaagtcga tccccgtaaa
    15361 gttgaggcta acttagaaaa tggcgtctta accgtttctc taacaaaaac taaaccggaa
    15421 aatattgccg ttaattttaa agactgatta tgaattacga attgacagta gccggattca
    15481 gcgatgacca agtaatactt aaagatgaca acaattccct tatctactgg ccaaaaaata
    15541 aattgcctca gataccggaa atcgggcaaa aaataaactt ttctatcggc gataacagag
    15601 ccgaaattct caacgagctc ctacgcactg ataattagtg acgccaatat gccaaaaaaa
    15661 cacttaccca tcatcgccat catcgccgta ttggtggtgt ttttattttt aatcgtcttg
    15721 acggctaatc tcggaaaatc agactcaact ttaagcgaca atttgtattt cggcggttta
    15781 gacattagcg gtctagataa ggcgcaggcg gaaataatga taaaaaaaga atatcagaca
    15841 atggttgaga agggcttaac tctaaaatat ggagattcgt ttctatcttt accggcgacg
    15901 tccgcatctt ttgacgccga tttgtcctat ccccactttc attatacgga cgataatatc
    15961 aaagcctctt tggaagctca caccaagccg ggattactag cacgctggtg gcggcaatta
    16021 tttggaccta aaaacatata tatcactccg accttcaagt tagatgaaga aaaaatcaaa
    16081 aaaaccgttc aaacctcatt aaccgacgcc ttttatccgg cggtggacgc tgcttttgtt
    16141 tttacaaaaa acaatattga aattaccccc gaaagaccgg gtaaaaccat cgactgggag
    16201 gcgttgttta aagatattaa aaataggctg gaattattta ccaccgaccc tatagtggtt
    16261 aaaactaaaa cggagtatcc cgaaatttat gcgcgcgact taatcggtct ggaaaatgaa
    16321 gcggataatc tcagtcgtaa tccattaact ctcgtcttca atgataaatc ttggccggtt
    16381 actcgagaag agatagcttc ctggctatat gtgcgtaaac aagacggtaa attaaatctc
    16441 gccttcagtc ctgaaagaat cgctaagtat ctcaacgaca atatagccga taaaataaac
    16501 gtctcaccac aagatccgcg ctttgaaatt aaagacaata aagtctccag ttggcaatta
    16561 ggaaaagacg gctacgaact tgactctatt gccagcgctt ccgaaattat caaccaatat
    16621 tttgcctctt ctgaaaaaaa tgaaaaaaat atttccttaa tcgttaaaac attgcctcca
    16681 gcgattgcag aagactttga tattaaagaa ataatcggta ccggccattc caattttgcg
    16741 ggctcctcgg ctaaccgtcg tcataatata gaagtagggg cggcggcagt caacggcata
    16801 cttattaaac cgggagaaga attttctttg gtaaaaactc taggagcaat caatgctagt
    16861 accggctatc tccctgagct agtaattaaa aacaataaaa ccatacctga atacggcggc
    16921 ggcctctgcc aaataggcac cactattttc cgttcagctc tagcctcggg cctgcctatt
    16981 accgccagac aaaaccactc ctaccgagtt tcctattacg aaccagccgg taccgatgcg
    17041 gctgtctacg atccttggcc cgatgtccgc ttcattaatg atactccgaa ggctatcctc
    17101 atccagtcac ggattgaggg taatgatatc tattttgatt tttggggaac aaaagacggc
    17161 agaagcgtca ccaccaccac cccggtaata tataatattg ttaaaccgcc agctactaaa
    17221 atagtagaaa gtgacgaatt atcgccagga gaaaagaaat gcacagaaca agcccataac
    17281 ggagccgaca cctatttcga ctacaccgtc atctacccag agggagcaac cagcaccccg
    17341 ctggaaaaaa ccagacgatt tagctcccac tatgttcctt ggcaagaggt ctgtttggtt
    17401 ggcaaaattg ccacttcaac gcctgataca gaaacagcct cttctagccc gactgtcgaa
    17461 accggttcta tagaaataaa taattaataa caaaaacgcg attattctga taaccgcgtt
    17521 tttatataaa aaagaccgtc tcaaacgggt aggcaagaaa atttcttcta ggaattaccg
    17581 agcccaccat tggcgcctga taaaagcgac aagggtaggc ccaaaaaacc ctagcaagaa
    17641 agtccccatg cttgtcgtgc ctgacccgtt tcaaacggcc tttttgtatt ttaagcaacc
    17701 atttaatata tcacttaaaa aacactttgt caatagtgtg ttattaacag cgaaacaaag
    17761 actgttgcta ccagggctta gcggtatata attggctatt tttagaataa aataatacat
    17821 caccatcctc atcggttcgc catatttgaa taccatctgc ccgcaccctg tgaataagtt
    17881 cagcggcagg atgaccgtaa ttattaaccc caacagaaat aatgaacgct ttaggattaa
    17941 ctgctttaat aaaatcgtcg ctattagcat tagcggatcc gtggtgagag gcttttagta
    18001 tatgcgcccc caaataatca ttttctgcca ataatgcctg ttctctacgg ccgctagcat
    18061 ctccggtcgc agtcaaactt aaaccggcgc aattaaaatt aatagcaaga gacagatcat
    18121 tgtcatcggt gctatctaaa aattctttac caggaggaaa taaacaaaga cgacaggcaa
    18181 tttctaattc aaaacaaaaa ttttccatag gataactgat aatagtcccg gactcagaca
    18241 aagaagatga taaagcggat ccggcataac cagccaaacg ggcgggcaga aaagcgcgct
    18301 taacttgata gcgttccact aactccggta aagcggctaa atgatcatca tgaccatggc
    18361 ttaaaattaa taaatcaata actggttgat gtccggaaaa ataccgaccc aaagcgtaaa
    18421 gggtagacca atcaggtcca ccatctataa gaatattcaa tcctcccggc gtatgcaaaa
    18481 gaaaggaatc accttgaccg acattaaaaa ataccacctc cgctctatga ggccgaaaat
    18541 tggaaaaaga aaaaattacc gacacaacaa aaattataaa aataaaaagg cgcatgaatt
    18601 gattctaaca aaaacctgat aagcaggcga taaaaaatta atcaacaaca tacaaaaacc
    18661 gccctgacgg gcggcctttg tataattttc aaaaattaat aaaatcagag ttttatttct
    18721 tctttgcaac tttcttcaca gccttcttag gggccttctt tgcaactttc ttcacagcct
    18781 tcttcacaac tttcttttta gtagccatag ttatcacctc cttcctggga aatttcccat
    18841 ttatattttt ctaaataaat ttaaaaaatt ttttttagaa atatttttta ctagttgttt
    18901 atattatacc gcgcgcgcca atttatagca atagttttga ctcaaaaaag aaccgccact
    18961 cttttttaaa aatttaatca tccctaatat tatcttttaa aatttatctt gaaaatattt
    19021 ttttatattt tcaactttta tttttgtttg ctgcatgcta tcgcgctcgc gtacggtcac
    19081 ctctcctttt tctaaagtat caaagtcaac tgtaacgcaa taaggagtgc ctatttcatc
    19141 ctgacgacga taacgcttac caatattacc attatcatca aactcgcaac gccaatttaa
    19201 tttcaaaaaa tcaaaaattt cccgcgctct aaccactaat tccggcttat tcttaaggag
    19261 gggaaagata gcaatcttaa ttggcgctaa tttagaaggt agggctaaaa ccgtcctcaa
    19321 ctcctcaccg acccgctctt ctcgatagct ttctgttaaa atagctaaga aagtccgacc
    19381 gactcccgcc gaagtttcga taatatgcgg tatatatttt tcattactaa tgggatcaag
    19441 gtagtccaaa tctattcccg aagattggct gtggaccgat aaatcataat cgcctcgagc
    19501 gtgtactcct tccagctctt taaagccaaa cggaaattta tattcaatat cataagcctc
    19561 ctgggcataa aaaactaaat tttcatgttt atgccactgc aaattttttt ccttaatccc
    19621 taaatccaaa taaaattgcc agcgtgtttg ccgccactgc tcaaaatatt tcatatcatc
    19681 acccggtttt acaaaatact gcatttccat ctgctcaaat tcacgagtac gaaaaataaa
    19741 ttgccgagtg gtaatttcat tgcggaaggc cttaccaatc tgagcaatac caaaaggaat
    19801 tttaacgcgc gtcgaattta aaacattttt ataattaaca taaataccct gacaagtttc
    19861 gccccgcaaa taaatcggct ctttactcca atcaccggta aaattaccaa gattggattg
    19921 aactaataag ttaaaattct tgccgggagt aaaatcactt gaaccacagt ttggacattt
    19981 gattttactt ttattgttct caaaaatatt attgatttct tcttcggaca tcttttcgtc
    20041 agccaaaata ccagcctcct caagcaaggt atcgagacga agacggctat gacattgacg
    20101 acattccgtt aagggatcgg aaaaaccctt tacgtgcccg gatgcttgcc acacttttgg
    20161 gctcataaat atggctgcat ccagaccaac gatgtcatcg cgaagttgcg tcatggctcg
    20221 ccaccattct tttttaatat tattggcaag ttcaacgccg taaggaccaa aatcataaac
    20281 cgccgtaaaa ccgtcataaa tttctgaaga aggaaaaaca aaaccacggc gtttacaaag
    20341 agcaactatc gaatctaaag aattgtctgc gtctttttta ctcatatatc taaaaatttt
    20401 tataattaaa aatattttac taactcttac atttcctcca aataatcaaa acctaataga
    20461 gaaaaaccat ttaccaatac ctgccgaaga gaacgcaaga ggccaatccg agcggaacta
    20521 agtgctggct ccgcctttaa aatagccact tcatgataat aatcattaaa aacctgagat
    20581 aagtcaaaga gataacgggc taaaattgag ggatcacgtt ggaaaccggc tttagcaacc
    20641 gcttccggaa aacgttgcat cattattgct aaagacagtt ctttaccatg aattaataga
    20701 gaataatcag ccgtcagcgt ttcatttttt ctcaaaatgc tggctaaacg ggcgccggta
    20761 tattgtaaat aggcggcggt aaatccgtcg aaacgcagag cctcatccat atcaaaagta
    20821 atgattttat cagccctgac ttttagcatt tcaaatttta aagccgccac cgctaatttt
    20881 tccgccgtag actcaatacg tttaatttcc caatcttgat gacgggcaac tacttcatgc
    20941 cgagtgcggg tataagcttc atcccaaatc tggctataag tgatgacatt accgttgcga
    21001 gaagacatca taccgcccct aagagtgaca aaatcatagc cgagatggct aatcttcaaa
    21061 ttttcaccgg ctaattcaaa tattttcgct aattgtttga aatataatcc ctgacgaaca
    21121 tcaacgatat agacggattc atctaaatga tactcgttaa atttcagcct agctaaagcc
    21181 aagtcggcga ccggatataa agcggtaccg tccgaacgaa taataggcaa aacgcctaat
    21241 ccatattctt ccaaatcggc aataaccgct ccttggttat tttttaatat ccctctctgc
    21301 aaaaattctg caaccatttt taagccgtca tcgattactt cgctttcgta aaaaatatgt
    21361 tcaaatctaa cgccaaaacg ctgataaatt tcggcaaaat aagcgatact ccaagctctg
    21421 gtttcctgcc acaactcata gttttttcct tgccggcttt caatctcttt catcacggta
    21481 gaaatttccg tcttcatctt ttctttttct aaaccgccgt cttcttttat ttcggcttgt
    21541 aataataaat tcgaggcttc ggcataacat ttgcccaaaa gccagccctt gggagcatct
    21601 acatctttat aattggcgcg ctgccattgc cataaagtct tagcgacatg aataccgaaa
    21661 tcattaatat aagaaacagg aatcgcccga taatcattaa aagccaatat tctaaaaaca
    21721 gcatctccaa aaaaaaggtt gcgcaaatgg ccgatatgat attccttatg ggtgttagca
    21781 ttagagtatt ccaacatgac tttttgctgg cgaccgacag ccagtcggcc gaaatcagga
    21841 cccgcctttt ctatatcaaa caaagcctcc cggttaaaaa ctccggcttt aaagaagaaa
    21901 ttaagataag ggccgacagc acgaaaagac tggatagctt ccggtaaatc tgattgttcc
    21961 aataatttta ttttctcttg agccaaagcg gccggactta agcctgaact tttagctaaa
    22021 gcaaataatg gcaaacttaa atctcctaat tccgctggca cgggataaac gaaatcatcg
    22081 gccgaaaccg ataataaaga agctaatctc tcttttagat tggctaaaaa cataatctct
    22141 aagctaagaa attttaataa attggcgctt gccgcgctgg ataattaagc caggagaaac
    22201 cacaatctcc gcctgactgt ctgtcagcgt tttcatatta ttgaccaaac cgactttaat
    22261 gccgccttga tcaactaaac gacgcgcttc cccccggctg gaacataaat tcaaagcgac
    22321 taataaatca gcaatcttat aattaccagc gggcacaacc ttactctcca cctcgtctgg
    22381 catctcctta ttttgaacgg ttttaataaa ataatcctga gaagcgtcgg cgtctttctc
    22441 gccatgatta atgcgggtaa tctcccaagc aagcttcatt ttaacatcgc gcggattgag
    22501 tttgcccgat gacaatccgg cttgaatatc tttcacttcc gcccaaggta aaaaggtaca
    22561 caattcaaaa gcggacggca gaatatcatc agcccaagac atcacctgac cgtacatatt
    22621 attaggactc tcatccaaca ttacaatatt accttcgctt ttgcccatct tcttaccttc
    22681 actgtcagtt aaaagcttga gtgttaaaac gtctttatct ttctgcttca atgccttaag
    22741 taaatcccgt ccgcacatca tattaaataa ttgatcatta cctccgactt caacatcgac
    22801 atctaaaact atgctatcat aagcttgggc aagaggatat aaaaattcat gtaaaaatat
    22861 cggtttttct tcctctaatc ttttctgaaa catgtcccgc tgaatcatct gctgaactgt
    22921 aaaattagaa gctaaaccaa gcaaatcctt aaaagaaagc tgatcatgcc actcgtgatt
    22981 atatcttaac tccgccggat tatcgccagt aaaattaaga taagctccgg cctgcttttg
    23041 aaaattaact gcgtttgccg tcacttcttc tctgcttaat tgtttacgaa ccgatccctt
    23101 atcggtagga tcgccaatca taccggtaaa agtgccaacc agaaaaataa ctttatgtcc
    23161 caagcgttga aattgagcca gtttgattaa agcaatagca ttcccgattt gcaaagaaga
    23221 agcgctcgga tcaaagccac aatacagtcg taaaacttca ccacttttca gttttttttc
    23281 taaattttcc cggttaggat aaatcttttc gactcctctt tctaaaattt ccgcaattaa
    23341 attcgagtcc gtgttgacct tggccataaa tacttgataa taatttatta aatatgttat
    23401 aatagtagca aacctagctg aatttttcaa ttaaataaag acgattttgg ttttaaataa
    23461 ttatcttaat aaaactaaca caatccttta tgttttctta tcgctttttt cttaagcaag
    23521 cctggaatat cactaagcgc tatcgctatc tctggttctt tggacttttt gcctctctca
    23581 tcgctcttgg tggagaatac cagataattt ctcaaggatt aaataataat cctggcggca
    23641 gcttcgtcag catcggcgtc ttcatgctct ataatctttt taaccccagt ttctacgccg
    23701 gtatcggttc tttggcggcc agtaacccgg ccgctctctg gtctttgatc acagccctac
    23761 tcttggctct agctttaata attataatgg tatatctggc catagtttct caggccgcct
    23821 tggttgaaca tagcgcccaa ataatattaa acaaaaaaaa gaaaattggg cttagcatca
    23881 atgagggact tgcttccgga cgcaaacatt tttggagctt actcggactc aacatcagcg
    23941 gtaatatcat tattaccttc agcttcttaa ttatcagtct ccccctggtc tttctcttca
    24001 tcacggacac aagcgtatta actttagcct acattctgct gtttgtcatt tttgttccga
    24061 tttctttatc tatagccttt atcattaaat atgctatcgc cgccagagtc ttagaaaacc
    24121 taagttttgt cgccgcgatg gagaagggct ggaaaatatt tcaaaataac tggttagtaa
    24181 gtttggaaat gtcattaatc cttttcttaa taaattttgc tgtaggtatt gtaacgattg
    24241 ccgttatttc tctttttttc ttaccgctac tattactttc tctccaatta tatctgcccc
    24301 tactcacagc cctaagcttt atactgaccc tcgccgccat gctggtcgcc gcctccattt
    24361 tcaatacttt ccaaatttcc acttggacca atctttatat ccacttacaa gaaaagaagg
    24421 gtttaagcaa attagaaaga atcttccagc gccagcgcta atataaattt tcaattatct
    24481 taaacagccg cggccaaagc cgccgattat cggcgcacag gccgcggcta ttaaataaga
    24541 cgctatgagc gatattgctt caatagaaaa tttaattgac ccagataccg ccaaagacga
    24601 agatagcgtc cagggtaaat ttgccaaaaa acagcaggaa ataaaactca aagagataga
    24661 aaaacaaaca gaacagaacg ccgccgccat tggcctaccc tatgtcaatc tttttggctt
    24721 ccctatcagc ccagaggctt tagttttgat tccggaagag cgatcgcgtg aacttaaggc
    24781 ggtttgtttt ttttatgacg gcaaaaatat ccgcgtcgcc tgtctcgaac caaacgaaga
    24841 gattgctttg gaaattcaac gtttaaatga acaatatttt gccgacacca agctctattt
    24901 gatttctcaa aatagcctta atcaggcttt ggaagcatat aaagccgtac ctaaagtcaa
    24961 aaaatatgat tccggggtgg aaataagcga agaaagcctg gaaaaattca aacaagatat
    25021 cgctagttat aaaaatttga atgaaaaaat aaatgaagta aatgtcagcg atattgtcac
    25081 tctgttatta gcgacagcaa ttaaagtcgg ctccagcgat attcacatcg aagctgagga
    25141 aaatggagtc attatccgtt tccgtatcga cggcgttctc caagaagcgg ccgtcattga
    25201 taaggaaaag tggaaaaaaa ttatcacgcg catgaaaatt ctagcaaagg ttaaaattaa
    25261 tatttctaat aaaccacaag acggacgcta tactatctat ctcaaagacg gaaaaatcga
    25321 cgtccgctca tcttttttgc ccaccgccta cggcgaaagc gtagtgatgc gcttgcttaa
    25381 ttctaaggca gtcgctctgg aatttgaaag cttgggaata cgtccggaaa tattggaaaa
    25441 actgaaacag gaaatcagca aacccaatgg tctcattctt accaccggtc ccactggttc
    25501 cggaaaaact acaactttat acgctatttt gaatatactt aatcagcccg gcactaaaat
    25561 agtcacctta gaagacccag ttgaatacca gttagccggg attaatcaaa gtcaagttga
    25621 cgccagcaaa ggctacactt tttcagacgg actacgctcc atcctacgcc aagatccgaa
    25681 tgtcattatg gtcggcgaaa tccgcgatct agaaaccgcc gaaatagcca ttcaagcatc
    25741 acttaccgga catttggtac tttccactct acatactaat gatgccgccg gtgttatccc
    25801 gcgcctaatc gacatcggag ttaagcccta tttacttgta ccctctatta acgccgttat
    25861 tagtcaacgc ttggtcagaa aactatgccc atattgtcga caggaacacc gactttcaat
    25921 ggaagaggaa gaaaaagtac aaaaaatatt agcggttatt tctccgcgcg ctaaggtcaa
    25981 taccccttct aaattaccaa ctatttataa agctggcgcc ggctgcgaac aatgcaatta
    26041 cttaggatat aaaggtcgta tcggaatcta cgaaaccttc accatggacg acaacttaaa
    26101 acagctaacc attgataaag caccttcttt taagattctt cagcaagcca ttgaaaacgg
    26161 catgattacc atgcttcaag atggcgtctt aaaggttatg gatggtatta cttctctgga
    26221 agaagtatac cgtgttattg gtaatttcaa ttatgtcgac gaactgtacg atatcgttat
    26281 ctcccagaca atcggtcgtg gcgtaaaaat tacagcggcg gaatacgagc gcggtcaagc
    26341 tttaactaag catatcgggc aagccattaa tctcagtgat attccctcga aagaattaat
    26401 taagatgata atgagtatgg ccagcgccag tgacgccggc gatgtccaca ttgaaccgga
    26461 agaaaatggg gtagctgttc gcttccgcat agacggtgtg atgcatgata ttctcgatct
    26521 acctaaaacc agctatttac cgattctttc ggaagtaaaa attttagccg gattcgccac
    26581 caatatcaaa agagcgactt tagatggtcg tttcagcgtc tacctgccag aaaagaaatt
    26641 cgactgccgc ctctcaataa tctccggcgg ttacggcgaa acgattgtta tccgcctcct
    26701 aataaattcc gccgcttcgc ttgacttgga aaaattagga atcacttcag tggccaagaa
    26761 agagctagac aaggcaataa caaaaaccaa gggcatcatc attgctaccg gtcccactgg
    26821 ttccggtaaa acgacaaccc tttacggcat tcttaataag ctgaataaat ccgatgttaa
    26881 aataattacc atcgaagacc cgattgaata ccaactcccg ggaataatgc aaacgcaaat
    26941 tgacactgaa cgcggatata cttttgcggc cgctatgcgc tctttgctta gacaaaaccc
    27001 caatatcatg atgattggcg aaattcgcga cgaagaaacg gcaaaaattg ccattgaagc
    27061 ggctatgact ggacatcttg tcttgtcgac aattcacgcc aatagtgccg ccggtgccat
    27121 ttcccgcttc gccggcctag gggtcgacaa atcatccctg gctaacgcca tcgaattttc
    27181 catcggtcaa cgtctagccc gtagaatctg tccgcattgc aaaattgaag acactcctgg
    27241 gcaagaaatg attgaacagg ctaaatcaat tttatcaagc atcaagaaca aagaagtagt
    27301 tattcctaaa acccttaaat tctacaaagg cgccggttgt gaacaatgta atcacttggg
    27361 gtataaaggc agagtgggct tatacgaaac cattccttct tccaactcaa tccgcaaatt
    27421 aatccagaaa gaagagacga ctgactacga tattgaacaa gaagcaatca acgaaggtac
    27481 gatcagcatc gctcaagatg gggtgcttaa agccttagcc ggagaaacca gcctagaaga
    27541 agtgttccgc gtaatttaat ttaaaaagct aatgcctcta ctaaagagtc aaatcatatg
    27601 attaacaccg cttttatcac taagttgaaa aatgactacc gccagcgcga acaagaaaga
    27661 cgacagataa tcagccgttc caacgatgtt ctctttcaag caaaaaaaac catcttcgcc
    27721 ctccagcgcg aagaactatc cttagctgaa gaaaaactgc ggagcatgga agccgttctg
    27781 aaaaaaatgg aaaaagattt tagccccaat cgccttcgcg aagaaggagc ctaccacgcc
    27841 gccgctgaag aatatgttga agcaaaaaca ttggcggcga tagtgtccgg taaaaaaatt
    27901 aatgccatca ccggcctgca cctaggatac gacagctatc tcggcggact ctgcgattta
    27961 atcggagaaa tggtgcgcct agctaccaac cgcgccgcta aaggaaaata tgaaagtgtg
    28021 gcggaactga aagataaagc tgaagaaata atgaacagcc tgcttgattt tgacttaacc
    28081 ggctatctgc gcaccaaata cgaccaggcg cgcggccatt tgcgtaaatt agaacagatg
    28141 gcttatgaaa tacgtctgcg taaagataaa taatggttat gctcatcgcc atcgctgccg
    28201 atttacatga taatcttgct aactggcaga aatttaaaga atactcaaaa aagcagggca
    28261 tagataccct gcttttctgc ggcgatttat gtaaccgcga cactttaaaa aaaatagcaa
    28321 ctaattggaa aggaaaaata tttatgattg ccggtaacca agaattatat aatcaggcag
    28381 aaactaaaac tttaccctct attagcttcc tcaatcgcta tggagtgata gaaataaata
    28441 atttaaaaat aggcttgatt catgaacctg aatatgaaaa aagaatcaca agagaaaata
    28501 aagggctgaa ctatttgttt tacggccaca ctcataaacc ttggtttgtc agaaaaaatg
    28561 acatcattat tgctaatccg ggaacacttg gcggggtttt ctataaagcc agttttgccg
    28621 ttcttgacac tgataccggt aaattagagt taaaaatttt agaaaatatt taaatgatag
    28681 aaccaaaaaa ctaaaagttt tttctataaa attataatag cgacagatta caaataaaca
    28741 tcgcgcaaat atcgaccggt aacactttct ttaattttca taacctcaac aggcgtgccg
    28801 gcggcaacaa tatatccacc ctgggcgccg ccttccggac ccaaatcaat aatccaatcg
    28861 accgatttaa tcacatcaag attatgctca ataattaaga cagtattacc ttggtcaacc
    28921 aacatattta agactttaag caggcgatca atgtcagcaa aatgcaaacc ggtcgtcggt
    28981 tcgtccaaaa tatatagagt gcgtccggta gcccgccgcg acaattctgt tgccaactta
    29041 atacgctgag cctcacctcc ggaaaaagta gtggcagatt gtcccaggtg aatatagccc
    29101 aatccgactt cttctaaagt ccctaatttc tggcgcaatg cagaattatc cttaaaaaat
    29161 tcaaaagcct cgctgacggt catctctaaa acttcatgga tatttttacc tttatactta
    29221 atatccaaga ttttttgctg aaaacgctga ccgtgacaaa catcgcaagt aagataaaca
    29281 tcatttaaaa actgcatttc caccttaatc gctccgtctc cagcacaggt ttcacagcgt
    29341 ccgccggcaa cattaaaaga aaaatgacca gaagttaatc gctttaaacg agcttcaggt
    29401 aattcggcaa ataagtcacg aatataggta taaagcccgg tataagtcgc cggattggaa
    29461 cgaggcgttc tgccgatagg agattgatta atatctatca ccttatcaat attatctaaa
    29521 cctctgattt ccttatgggc gccgacatcg actttagctc ggtagaattt tttatttaaa
    29581 gcattagcca gaatatcaga cattaaagtc gatttaccgg aaccggaaac tccggtaata
    29641 gccacaaatt tacccaaagg aatatgaaca tcaatatttt ttagattatg ctcgctggcg
    29701 ccagctaccg tcagaaattt gccatttccg ggacgaaaag attcgcgctt ggcaatagac
    29761 ttaaccccgg agagataagc tccggtcaat gactgacgac aatctttaat atcctcaggt
    29821 ttaccggaaa acagtatttt gccgccatgt tcaccggcgc ctggaccgac atcgactaaa
    29881 taatcagctg cccgcatcat cgcagcatca tgttctacga caataaccgt attgccatta
    29941 tcacgtaaat ctttaagcat ggcgattaat ttatcattgt cccgttggtg caaaccgatg
    30001 gaaggctcat ctaaaacata gagtacgcca cttaaagtgg agcctacctg ggcagccaaa
    30061 cggatacgct gagcctcgcc tccagaaaga ctattggcgg gacgactgag ggtgagataa
    30121 gacaagccga ctctttccaa aaattctaaa tttttaataa tctcttttaa aatagggtga
    30181 ataaccgcct gattgccgtt ccaagaagaa gataacagcc atttagaaag tagaggcctc
    30241 aggatatcaa tggttttatc ggcgatatca gcaatgctca agtttttaat tttagcagcc
    30301 agatattccg gttttaaacg aaaaccgcca caagctggac aaactgccaa gcgcatacat
    30361 ttttcaattt cctgtttaac gaaattggat ttcgtttttt catagcgctc taaaagctca
    30421 gcattcaatc cccgccactg cttgtctccg tttaatatag ctgcccgttg accgacgctc
    30481 atctcctgca aaggtgtttc caaaaaaaaa ccatagcgtt ctcccaactt tttcaattct
    30541 acaaataaag cgctgctggt attgagatta gcatgagcca ggggtttaat cgctccttga
    30601 agcaaattaa gtttcaaact aaaaactaat tccgggtcaa tttctgtccg cgttcccaaa
    30661 ccaccgcagt gcggacaagc accataattt gaattaaaag aaaaatttct cggttccagt
    30721 ccgggaagag tgaaactgca ggtggggcaa gcatagacat aatgacttgg ttcttgcttc
    30781 ttcttgtttt tgcgcccgga acttttttcc gcctttttca agcgcgtctg acaatgaggg
    30841 caataaggcc aagcgactcg ggcataaagc aagcgtaaat aatcataaat ttccgtaacc
    30901 gtaccgactg tagaacgcgg attattgccg gctgtacgct gatcaatggc gatagctggc
    30961 gacaagcctt caatcaaatc aaaatctggt ttatccatta atccgacaaa ctgacgagcg
    31021 taggctgata aagattcaac ataacgtcgc tgcccctccg cgtaaatagt atcaaaagct
    31081 aaagaagatt taccggaccc agacaagccg gtaataacaa tcaacttatt gcggggcaaa
    31141 tcaagattaa tatcttttaa attatgagta cgggcgcctt tgatttttat attattttca
    31201 agcatgtctg aataaataaa tattttaata gtgtccatta tatacttaaa tgcgcaaaaa
    31261 aacaagagcg gatttaaacc gctcttggaa aaaatgatta aaaaaatgga aagaaaatcc
    31321 gcaaaataac catcagccaa cgatggtgcc aaactagacg ctgagaggat aaatttatct
    31381 tgacagcgct atcgcgccag cgtcgccaaa tcttaagcaa gtcggctacc gcttgttttt
    31441 gtttaaagaa aacccctaat tcaagattaa agttgaaaga caaaatatcc agattttgcg
    31501 aaccaaccag ggcttcctta tcatctatta tcatcacctt cgcgtggttc attgcctgag
    31561 ttaaataaaa aattacaccg accgaggata aataggaagc gttagcataa ttaatcttat
    31621 ttaaaaattt gatatccgtg tcttggggaa tgattatctc tactgatacc ccgcgatgaa
    31681 tggcattatc caaagaagct aacaaccaac gcggtggcat aagataaggt gtaacgattt
    31741 taatagaagt ttgcgcctcc atcaatttac ggcgataata attattaaaa ctatattttt
    31801 tttcacctat ttcccaagtg tctattaccc atgattttat tttttttact aaaggcagac
    31861 gactatgaat aagaatacgc tcatctttgc cgccgcaacg tttgtaagta taggcgaaag
    31921 aacgcaataa ggggcgaaca acccgtccgc gcaagcaaat ttgtaaatca taccaatagc
    31981 gaatcttgtc ctcaatatta acgccgccta aaaaagcaat tttttcatcg ataatcacca
    32041 gtttgcgatg aatgcgctta aaccaacggg aaaaaaagat aaactcgacg ccagcgtttc
    32101 ttaattcaga caccgatact gactttaaac taatactgcc ataagcatcg acaataacta
    32161 ccacctttag accggcctgc gccttttctt ttaacttgcc taaaaaatca tgagtctgac
    32221 gagtgtcgtc caaaaaaata tacatctcaa tataaatcga tttttccgcc tttaccaaag
    32281 cggccaaaat tccatcccag gccttttgtg aagttgtata gaatttatag cgcataaaat
    32341 tattatacca cgggcgggga aaaaataggc aaacaaaaac caggctgtta acctggttta
    32401 tatattttat atatatttta tgataatagg cgtttaattt ccggcaaaaa acgcttagct
    32461 tcgctgcgac cgatatcaat caaagcttta gctaccggcg gatcaaaata ttttttatac
    32521 tcattggtct gcataaaagc agaagtgtct ggattcatta ctaaatctgc tccaaataaa
    32581 gaatgcttgg ctagattatg gacggcaatc ctagttgatc gcaaagccac cgtcggcaga
    32641 ttaaaacggc gatgaataaa ttcatgatga tgataaagat taacagcaat aattttatcc
    32701 gctcccatct ctcgtacaag attaaccggc accgggttgc tcaaaccgcc atccaccaat
    32761 aagtggccgt gaattcgcac cggctcaaaa accaagggga cggaagtgct agctctgacc
    32821 gctgtagcca aacgaccttg atcaaggatg caagcctgac cactaataag atcagtagcg
    32881 acaatcttta aaggtaaacg acaatcagaa aaatttttac gacccaacac tctctccaag
    32941 aatatttcaa atttacgtcc ggaaactaaa ccgccgcgaa aacccaaatc aaataaagtt
    33001 ggcaatttcc ccttcttgtt cataacaatt ttctgcttca ataaatcaaa actagaataa
    33061 atagaaaagt aagcgccaat caaagcacca atagaggcgc cgctgatata atcaaggggt
    33121 atattatttt cttgcaacac ctgaataacg ccgatatgag cgaagccgcg aaaaccaccg
    33181 ctaccaagtg ctaaaccaat tttaggacga gatttcatat attttagact gactatattc
    33241 tagtattttt tctttaaatg agcaaatata ggaggctaaa agtcttgttt aatttaatat
    33301 taaaataaaa atgcttaaaa aacattaatt aatccctgtc caaacataaa aagctattct
    33361 tatctaatag ctttttcata atcagtctta gggcctgatg tttccgaaaa aattgtttag
    33421 attattgggg aaattgagaa atggtcggaa attttaaata atagagcttg ttaacagttg
    33481 actataatat taacatatgt taatatagat tatataagtt aacataaaat atatgaaagc
    33541 agaaaaaaat tattataccg tcaaagaact tgctgatatt atgggtctta gtaggatcac
    33601 gatttttaaa agaattaaag ctgggaaaat taaagctgaa aaaataggta gaaattatat
    33661 tatctataaa aatgatttaa aagaaatgtt tagcaataac cttaccaagg acgataaaat
    33721 tatcattaaa aatgctttaa aaatagtctt aaatgaatat ggagacacga taagaatgct
    33781 gggtaaagaa taaaatatgt taaagccact aaccattaat gaaataaaat atttagctca
    33841 tcgtttagct attgacacta tgggttggga tgagccgata ccaaaatttg aaacaagata
    33901 ccccaatgta ttggagagtt gtattgctac accttttcag aaatttgaaa gaaaaaattt
    33961 gtacgcaggt attgatggta aaggggctat tttgttttat ttacttataa aaaatcaccc
    34021 ttttcaaaac ggaaacaaaa gactggccgt tgcgtcattg ctagtttttc tttataggaa
    34081 taataagtgg attgaagtag acaataaaga actttataac ttggctgttt gggtggccca
    34141 aagtccttct ttagctaaag atgaaatgat tatttttatt agtaaattta ttaaaaaata
    34201 tttacggaat tttaagctct ccgagaaata ggcggaaatt tttgaatagt taaaaatcta
    34261 ataagtattt aaagtgttca tttttaccaa taaaatatta ttatgcatac ttatgtaaat
    34321 agacttttac cacactttaa attatcttaa ttacataaaa attattaata tgttacaaaa
    34381 taataacatt tcagataaaa ataaggttaa attgattaac aatatttata acaaggctct
    34441 ctctgatata aataatatta aaaaagaacg ggatgataaa attataaaat tattaaaaaa
    34501 tatcgatgac cgtcatattg acaaaatttt aaaagatata aaaaatacta aatgataaaa
    34561 atatggattt cgagaataaa caaaataaaa tagaagaatt cgccccagag aaaaaaccag
    34621 aaaatgaaaa aaatctggaa atttacgatg ttattaataa tggagagaaa aggctttctg
    34681 gcgcctctga aaaaattagt aatgaaaata tacaggcgat ttctcatgta gaaaattatc
    34741 ccggcactga atcaagagat atagaaaata ctcaggggat aataactgaa actaatcaaa
    34801 aaattaatca agtaatagaa aatactcaaa aaaacgttag agaaattgtt ggcaaaaata
    34861 gtgaaactaa tcaagaaaaa gatctaaacc aagaaaaaag accatcacag gacgatttaa
    34921 gagaagtaaa tattataaaa agaatagata ataaattttc aggcagagaa aatagagatg
    34981 aattcttaaa tatatcaaga aaaataacag aaaaaattaa aggtttgcca gataataaaa
    35041 aagaagttta ttggcaaaaa atgttatccc tagttaagtc agagcaaaat aatgacattt
    35101 attattataa ggaattgcat gatgaggcct taaatgaatt aaaaatggtt gaaaaaaatt
    35161 ctagtcttaa aaatagggac atggcgatag aggacaggat tactaaatgt agctcatttg
    35221 aggatttaga tgttgtatta agtagttctg aaggtttgtc tggaagtagc aataattatg
    35281 attcagaaaa tttaaaaaat attatttctg atgtaaaaaa tttcaaatta ccattaaacg
    35341 ctttaaccag atcggctggc ttgcgtgata aagtgaaaaa tttacttgcc acacctgatc
    35401 ctttggtgac aacaaataga tttgaagtca caactgccga tgggacaaat atttgggaaa
    35461 gtcaaaatac cccaaatcca gaactaatta gattaaatcg gggtcgtcac tgggaattaa
    35521 ttagcaaatc tgaatacaaa gaaattttgc ataaagcaaa agaagatgaa ataggtgcat
    35581 atgtcgactt attggataaa tataagaagg aaaatttagg agaaaagagg aattcaatga
    35641 taaattttat taataatgaa tttattttta caaaaaatca tttgggtgaa attaaagaaa
    35701 atttatctat tttgtatgaa aaaacaaata cccctattta taaaaaatta cttgattata
    35761 ttaattcaag tgaaaataaa tttgatggta accaagaatc acctttttta aaaaagatta
    35821 aaagtttaaa ggagaaaagt aattgaaaat aaaatataaa aatccccact aaatagtaat
    35881 taattgggaa agtttttaat aaaaaattca atttaagctg ataaaaatta aatatatagg
    35941 tagaccaatt ggtaa
//
LOCUS       DAOQSA010000005        33843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_701_length_33843_cov_7.329525, whole genome shotgun sequence.
ACCESSION   DAOQSA010000005 DAOQSA010000000
VERSION     DAOQSA010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 33843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33843
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_701_length_33843_cov_7.329525"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4590
                     /locus_tag="PKK65_01040"
     CDS             <1..4590
                     /locus_tag="PKK65_01040"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNU81304.1"
                     /translation="TGTTTIPNVNKGEMSSFVVASIAGNNEKSNYVRFVKQAEPNQGP
                     YISYFEPSSGRAGQYVTIHGSGFGGARGSSRVFFGDKEASYDFPEVCASSVWKDNQVI
                     VKVPADINDGNYVLRMEINDDKIDSQNANPNVFTVDANSSLKTSICRIFPTRGQIGTP
                     VEIWGEYFGDNGGNALTVFSYNRSVSGQISLEQSAQKLNPSVPDNAVTGAVKVVKNSE
                     WGNDVNFEIGTCVDNSDCSGDTCCPAGTYKQGRCATSLAECYVDIPNSVFEWNFSTEF
                     GGVEGDDEFDSCQGMAQALGACQVGAFCPNSPGLCSPYAGGTKVLGSCDSSCATVPAC
                     AELGEGTCVYDHQSDRCFINNNTCSLDSNISYTLGQQTFNAVQSCKIFSQFGGQAHWQ
                     ISVASSCPNGWTRLDGGHCVDSTSSTESTCSLCPTNFNCRPIGQEGRCVSAELCPGSA
                     SCDGTSCLADTSARCDCCCEIGQDARDCCLPLTCSGTCGSDTSDDKAGLGQCSGCMID
                     SDGNGTISPEEQLLSDQACNCSAHSGKYCDISVPTGACVDCSRLSVQSCLNHSQSCCL
                     DSKGTASPADDVCRGGNGQEVSADPASPDFGYCAYYDCQNASSTPPGDPSLCASSTPK
                     VFGLFKNVEACVNNCAASPGLSFCGQFDGDLVACAAANGCCFNFNDQKCTSGAQMLGG
                     YCAYYNCQAAPNEAQCDPNPSPIGIYNSQTACSIGCAQPPFGGLGKDCRNLTTNNTDC
                     NQSFCSPPFACLNGSGNIGLPGDCGACCCQVGNPDSCSGIGNGSLVCQSNQTPCSGED
                     RGLCCGCSQDVDCGNANTLGCDASACCRNRPSVLGDQLVPPHGATGVCRNASIRVPFD
                     QLMDVGSVMDNILLLEEHDYGNGVCPAGTFLASVSTVNTINHNWLAKAYSQVKSSFRS
                     ILNRLGLSSAPVLALNPPAPDKLYCSIPGNISVINNGNGSVAEFIPKKLLSPATNYYL
                     IVKGDESLDSNAGVLSQWQIGLNGPGYLNLGTNSYIEGENIKFNNLTFKNSYIAGFTT
                     LPQQGANVGICAVDYVVTEPASYLFQSTDNDLNEDDSNPNSNSFDTKNDRDKLFKAGA
                     YSADDQLLHPSSGYYWDWSWQVAKPSIASIAYVSGLADNQAFVVAADGVSDDSTILSA
                     QIDMNRFSAPNCNSSASCVCAEPECVNNCCNIYQEGDGLQAETPLFIFLCKNPWPAVN
                     PVTLAWSPWYDTCVGAIGNNCSNYNYKFYYCRDAGADGLADDLPAMINPAVIRGTSNS
                     LICSEGQTACSNLGTTCGPDNNYDGVGDGFCIWNILKESYFFKEATPQIGAITAAIDQ
                     EDGDSVRLEWYGDASVIYNANASLMGKYRLYYAPVSSGNMSFVDISPRDAYIVGVPGP
                     VCTPLTPANGENYSCHYLLKGLQAGENYRFRISAISSAQVESPLSEEKTVLITDTLAP
                     APPQGLTATIVNGQRLHFVWQANTDDTSFYRLYHGVSSGQYGESYDSDKQATTMDLNL
                     QQFSQGIHYFALSALDSSNNESNKSAQITIVVPAP"
     gene            4601..7618
                     /locus_tag="PKK65_01045"
     CDS             4601..7618
                     /locus_tag="PKK65_01045"
                     /inference="COORDINATES: protein motif:HMM:NF016584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dickkopf-related protein"
                     /protein_id="HNU81305.1"
                     /translation="MNKRGYFYALIIVGLFFSWAHCGQAAKSTGDEPGGQTINNTINN
                     TFIDDKDSDVIAVRVLPNPNHYSAARWYKSQGFSGSPQALLVDGYDAVRDGRTVYVNA
                     ANVDLATKNIYTNIYLISYNQNPQLKTVDVLGQIISHWRFNSNLTTAGNCYISTLLCE
                     NDSDCSQGQYCFGKTEAPGRCRPQENSVCYTDADCTQGLYCDSLRAKVARDVRRLGML
                     GDLRESLSAFKKINNKYPILGAGTYLPLSSISVWPSWQSLFLPQLGMPQSLIDPINKL
                     GPCAGYNSETCWNEETKAFADPNPQDNNLELPGGSYAFVYSGDSNGSNYTLCAAMETK
                     SLGYNTTEGQLAESGCVSSGAGYIGSGNNLAPVLIATNLQGQQDQEFNGFIKVIDPDG
                     NPMDWSINTAATVWTNWRNNGQNNVAPIMLDTSNPHQKKIYAQAAGNPGTYNLSLSVA
                     DGQGGVLSTVTPITIVNTAPLIQSDDIYYYPSTVIPLTINFSLSDKDQPISYTITKAT
                     WNSGPYDLLAPANATFLSESHHQVGDTVYYTLKYNLHTSNKFTHDTTFVYVITAKDKY
                     NNTATRQLNINIKIDPPVLDFNCASSVRVGSYYSCRLGFSKQGDHTITYRAVGSLPSG
                     LSIGEVMQPGGGVGGGSIQASRGSLWRFISKLSSWFKAAEAAVANTSYALQGTPTTAA
                     SAVSIKIKAENEFGAVSEREFLLNINNYCGDGVRQAPNSEGRGGFYNDGHEDCDGNSG
                     IISDRSQIGSTSPTLQYGCTTKLGSAVPYPIMNTYYCIYLSADDPNGGGYCGDGICQA
                     RIIRNNQLTPWEIGGYCLEDCTCAGDHLVPVGDSCECETGWYDCDSEALGCESEKPCD
                     GCGADQYECGNQCFSKNSFDCGLYSSKCYSNSGTSCTTSCPSGYYNCNGTNECESTSC
                     VCPDGQHWEANVWPDGACVANTPAGCPNGMLQCGTTGLNCYNPDTQQCCSNSTVCGIN
                     QYCCNVSSQILTCVNNYVTCYIIMSGGNSGASN"
     gene            complement(7901..8932)
                     /locus_tag="PKK65_01050"
     CDS             complement(7901..8932)
                     /locus_tag="PKK65_01050"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HNU81306.1"
                     /translation="MTTSYQNYIGVDLHKKTAYITVMNLTGKIKKQGEISTDTDKVAK
                     FFSRYAGNNLVAVESTMNWLPFYENLESLGCQTVLSNPLATKAIAFSKMKNDKVDSRI
                     LADLLRTKMLPLAYIQPRDIRDLKELVRQRIQYVEMQTRIKNKIHSVLFKTNSKHEYT
                     NLYGVAGMNWLKALELRPIFRDELNRYLLTLDYLGKQIKELSDNIENTAKADYDCKLL
                     MTIPGISFFSALVIKSEIGDISRFSSANKLCSYAGLIPSTYASADKIRHGRITKRGSI
                     WLRKTLINAVSSNCRHANRLASFYRKLKKNKGTGKAKVATARKLCSIIFAMLTDQTIF
                     REFASVKQR"
     gene            9006..9317
                     /locus_tag="PKK65_01055"
     CDS             9006..9317
                     /locus_tag="PKK65_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HNU81307.1"
                     /translation="MKKNSIIIIGAVICLIVVFVLLYFFRDEPSTQTPLLNDVQPQSV
                     APVLMTAEEKNSLFIPVDKEVQVLRRNADGEIMTYKIIESQDQIINNGSLFPSNSSEL
                     K"
     gene            9405..10088
                     /locus_tag="PKK65_01060"
     CDS             9405..10088
                     /locus_tag="PKK65_01060"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HNU81308.1"
                     /translation="MFNDLNKQVPASNAVDDIFAETESTVRSSEQKVNIEAQSAGLSA
                     PFAGEDKSGDNSSGVKIKFILIIILALVILAAAAYLVYAKITRNAADQALDSPVLNNN
                     VAPIVKTDNSVGTVVMPEVQSPEVNATATTSDISPVVTPNIISATETPVAVTAPVDSD
                     GDSLTDAEETILGTNINLIDSDFDGLSDYEELRVYGTNPLNPDTDGDSYHDGDEVKNG
                     YNPKGVGAL"
     gene            10113..10298
                     /locus_tag="PKK65_01065"
     CDS             10113..10298
                     /locus_tag="PKK65_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81309.1"
                     /translation="MALANQKLNKKDSTVGGVRLYLRIHVMPVLNKPLRGRAWQWISP
                     FVALAILIVAIFYFLAD"
     gene            10300..11316
                     /locus_tag="PKK65_01070"
     CDS             10300..11316
                     /locus_tag="PKK65_01070"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HNU81310.1"
                     /translation="MDSKNKKMPNKDDQELQERLQKDFIVRNMPALTSLSGASYGDKE
                     EKGVNHRQAGDNGGKPKDNKHQSTGIIIISAGLIVAAGLFYAAYHFLILPAMRPSVAV
                     NTAPVAEVTESSVAEEEEKAQTATIVPETMVAPVVEELVNSVEPDSQDSNIVSLPIVV
                     DSDNDGLSDVSEVFLGTNPQLADSDSDSYPDKQEIIAGYNPLGGGKLSDNGFSIYVDP
                     NGFFVDVYPQNWTVNIVNQNSVLFAAPDQSFIQIAREESDQPYDNILAWYSSQFSDAS
                     TLDVSRFLEGNMGIGIISADQQIVYFLDRDRSHIFVVSYIKSTESMPYLEIFKTMAAV
                     LMFP"
     gene            complement(11409..11990)
                     /gene="clpP"
                     /locus_tag="PKK65_01075"
     CDS             complement(11409..11990)
                     /gene="clpP"
                     /locus_tag="PKK65_01075"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNU81311.1"
                     /translation="MTLIPTVIEKEGQVERAYDIYSRLLKDRIIFLGEEIDDHVANIV
                     IAQMLFLAAEDKDKDIKFYINSPGGSVSAGLAIYDTMQYIKCPVSTICVGLAASMASI
                     LLAAGAKGKRLALPNSEIMIHQVMGGAEGQASDIKIRAEHILKIKDKMNKILAQHTGQ
                     KINIIEKDSDRDNFMSAEEAKRYGLIDKIISKE"
     gene            12134..14020
                     /locus_tag="PKK65_01080"
     CDS             12134..14020
                     /locus_tag="PKK65_01080"
                     /inference="COORDINATES: protein motif:HMM:NF014416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNU81312.1"
                     /translation="MSQTQKFSNFMKRHHQRWLFLLVVVFAFLGFILAAGNYYNRGVQ
                     ALANATNNIIVLPKAPDSPFVLGLDLQGGTQLVYEADVAHIAEGDRLNSLEGVRDIIE
                     RRVNSAGVSEPIVQINRTLQGDYRVIVELAGIKDVNEAIKLIGETPLLEFKEQSTEAQ
                     AATVEPNAEAVAFNKQVEIKAQAALNDWKSSSDWQAIADKYQAKNEIKNDITADNEPA
                     LAAALQNLAPNTIVASPINEGQSLIIAKLIDKKEIDNPFKDESGQEPVITSYSAQLLS
                     FPLQDESPVLSLGDNWKNTELSGRNLKRATLQFNPQDNSPEVALEFDSEGGKMFADIT
                     ERNIGKPVAIFLDGYAISVPTVNDRIPDGRAVISGHFNVQEAKLLVQRLNTGALPVPI
                     NLIGQQTIGASLGQKSISNSVKAGLIGFTLVALFMIIFYRLPGLMSVISLGIYVLTVL
                     TIFKSTPIWLALLFILLLVLLFISVFNHLKIFDGVFSALLFLVILAFLVFYANQPVTL
                     TLAGITGFILSIGMAVDANVLIFERMKEELRSGKPLAAALDDGFKRAWPSIRDGNITT
                     ILTCFILMGFGTGLVKGFGATLFIGVSISMFSSIVITYILLKMFKSSWLDKHRWLLGF
                     TRTK"
     gene            14062..14958
                     /gene="secF"
                     /locus_tag="PKK65_01085"
     CDS             14062..14958
                     /gene="secF"
                     /locus_tag="PKK65_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNU81313.1"
                     /translation="MNLQIIKHRNLWLSLSGIMVVASIIFISLWGLKFGLDFTGGSLL
                     EVKFTSGQPSVSTVQAGLNDTQVSSLIIQPTEDSMILRFQESDEDTHQAVVNALRQLP
                     EAQAGLEEVRFEAIGPSIGQELRSKAFWLMFFVLIVIIIYIGLAFQRVSKPVASWKYG
                     LTAIIALFHDLLITIGVFSLLGHFYGVEINTTFVVALLTVLGYSVNDTIVVFDRTREN
                     LPKSTGSFAETVNSSLNQTLVRSINTVITTLLALLAVLFFGGDSIREFVLALIIGIFC
                     GAYSSIFVVSPLLVVWQRLQKR"
     gene            15047..17353
                     /locus_tag="PKK65_01090"
     CDS             15047..17353
                     /locus_tag="PKK65_01090"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNU81314.1"
                     /translation="MDRKSLNIKRILSFFLPSFYWLALLAVPLSVSYFFPIASPFVLI
                     KSAWLQILAALTLLVFLFFVVFRNGQSQVFFKEKSWWRAILPVWIFWLALSVLSFWSL
                     NPWQSWFGSYLRQTGILFYFWLAIWYSFIIYYFGGFYRPENRVVFWLRGVRLSALLMS
                     IAGAFAGLYAFLQFCGYDLVVWQESQLYSRAIGTLGQPNFLGSFLLFSLAMAAYYFFD
                     SKRFLNKFLALLMFLLQLAGLVVSASRAAWLALIVSTIILLVLFAWQRWRWRALVVAP
                     ILLWFFSTFFYLLMPARFSSLTDFNSGSLALRFSFYRASFQIIKENPWFGVGWENGEE
                     AFITHYQPEWGVLMNVDSLTDKAHNSVLDIIIQTGIIGLFFWMLLCIFWVWQCWRLYH
                     SSVGKKFSLASIFALLAYSLTLMVGISDISSIFYFWVIAALVSAGNLSLGKTGSSCVL
                     FARTKKNFRFFTRRPFNLSLGEWLIKIGGGVFGLLALGQIYFSLSALQADYYFLQIRR
                     LLPAREYFTVSVILSYLDRSALNPVAKSYYHTSLASYILSDWSNLEDLNSQYLLRHEL
                     SRILSQSFLPGYNERLLSARLQCFLSGPETASPLFAELIAASPQRPASYRDYAQCWQI
                     NGGNSEALSLYRDVLRLLPSADNSLLNDDHRAYLNFYISRIYTEMALAQENSGQLTQA
                     LESLRLAYSAYPEDLINLQKRADIYGRQKEYNLALFWLEHAYRRQPGSYYWPLAISNV
                     YNLLGEEALAASWYSQALILNPDLKPAP"
     gene            17369..18172
                     /locus_tag="PKK65_01095"
     CDS             17369..18172
                     /locus_tag="PKK65_01095"
                     /inference="COORDINATES: protein motif:HMM:NF018460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HNU81315.1"
                     /translation="MLLILIIIIALGLSFGSFANCLVWRLYRQEKISGRSYCPRCLKK
                     LWWHDNIPLFSYLRLGGRCRFCHQPISWQYPAVEVIVAGLFVFFWFKHLGFAWVDLET
                     LSLFFNFDFWLALIRDYLAVFVLTVVFVFDLRFYLVSNAVVIPAALVFAGLNLLLGAS
                     WYVLFLGIAGAVAFFGLQFLVTRGRGLGEGDIWLGLLLASLFPDWSLLILAILSAYLL
                     GTIVGLVLIIAGRKEWSSKLPLGVFLAVGALITLAWGPALLTAYWSFQL"
     gene            18267..19523
                     /gene="ftsA"
                     /locus_tag="PKK65_01100"
     CDS             18267..19523
                     /gene="ftsA"
                     /locus_tag="PKK65_01100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNU81316.1"
                     /translation="MKNDIIAGLDIGSTAVRLVIGQKISGPTGDEIQIIGAVSSPTAG
                     VSKGIVTSIEEATSSISACLEKAERIVGVPITSVWVGINDPNIKCERSKGVVAVSKSD
                     GEISEADVNRAIEAARALAVPANYEILHVMPIKFSVDNQIDIKDPIGMSGIRLEVEAL
                     IIQGLSTQTKNLTKAIFRTGLEIEDLVISPLAAAEAVLTPKQKDLGVALINIGSSTTS
                     LAVYEERNLLHAAVLPIGSEYITNDIALGLRCPINLAERIKLEYGSANPDAFESDDEV
                     DVTRLVKEEEVNDDISLVSRKYIAEIIEARVEEIFDKVDKELKKIDRSGMLPAGVVFT
                     GSGAQLDGMIEAAKRKLRLPAALGQSKNISVVIDKVKSPEFLPALGLVLWGAHEYPSQ
                     GSGNLRKNIGDIFGKAKNIFQKIIPR"
     gene            19582..20781
                     /gene="ftsZ"
                     /locus_tag="PKK65_01105"
     CDS             19582..20781
                     /gene="ftsZ"
                     /locus_tag="PKK65_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013172594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNU81317.1"
                     /translation="MAEVKPEIETFAKIKVVGVGGGGGSAINRMIGNGIRGVEFVAIN
                     TDIQALHYNKASEKIHIGKAVTKGLGAGMNPDLGKSAAEESQNEIREALKGCDMVFVT
                     CGLGGGTGSGASPIVAQIARDLGALTVAVVTKPFLFEGGQRKNIADRAFADLADKVDT
                     IITISNDKLLQVVDKRTSLLEAFAIADDVLRQGVQGIAEIITVPGVINADFADVKAVM
                     ANAGSALMGIGQASGENKAIEAAKAAINSNLLDMSIDGARGIVFTITGGPDLSMNEVS
                     DAAKVITSAADEDAKIIFGAVIDDKMKDQIKITVVATGFDGKSKSSKVELKSYTPNPF
                     VESENEKESTPASALWGNKKAKIPTIKNIPLQPLVEKRQEKMSEPEKTEEDDLSVPAF
                     IRKKMSN"
     gene            20793..21518
                     /locus_tag="PKK65_01110"
     CDS             20793..21518
                     /locus_tag="PKK65_01110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNU81318.1"
                     /translation="MNIDFCVPVYNEEEIFSANAERIWRFLQSFMCEYDWHLIFIVNG
                     SNQKFIDLVQDFVNKHGPQSACFIISESGKGRAIKFYFDQSKADFLVYMDIDLAVDLF
                     NLPQLLAPLLSDKADLCFGSRMLPASVKNRSWFREISSKVYLRLSRKLLGHRFTDLQC
                     GFKAVSQEAWKSITPLILDKAWFFDTELICLAQKKLLRLQEIPVDWSENRYIKRRSKV
                     KVWKEAWVFLFLTFKLRSRLQRM"
     gene            21722..22186
                     /gene="nrdR"
                     /locus_tag="PKK65_01115"
     CDS             21722..22186
                     /gene="nrdR"
                     /locus_tag="PKK65_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNU81319.1"
                     /translation="MKCPVCYYNDTKVVDSRVAPDGLSIRRRRECLKCNFRFSTYEEI
                     EILDLMIVKRDGRRESYSREKLVNGLRKSLEKRPVTEDNFKRIIHGIERDLQKLRQGE
                     VTSRQVGQIVMKHLKKMDQVAYIRYASVYESFKDANEFRRELNKLIKDKKEK"
     gene            22277..24328
                     /locus_tag="PKK65_01120"
     CDS             22277..24328
                     /locus_tag="PKK65_01120"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HNU81320.1"
                     /translation="MSEIISQIRKRSGDIVGFSQQKIKDAIYKAALSVGIDDEEMSAR
                     FSEEVVKKIAAKFHKNSIPAVEEIQDIVEEVLIEHRQIKTVRAYIIYRDQHRQLRELN
                     KATSDEKLMEDYLGKADWRLKENSNMSFSVQGLNNYIASSISARYWLNKLYPQTIRDA
                     HVNGDIHIHDLGLLAPYCCGWDLKDLLVKGFGGVPEKVQSKPAKHFRSALGQIVNFFY
                     TMQGEAAGAQAFANFDTYLAPFIRYDNLSYKEVKQGLQEFMFNINVPTRVGFQTPFTN
                     ITLDVTPSKLVGEENVIIGGQIMPEKYKDFQAEMDMFNQAFAEVMLEGDSTGRVFGFP
                     IPTYTIDKNFDWERECLKPVWEMTAKYGIPYFSNFVNSDMDRDDARSMCCRLRLDNRE
                     LRKRGGGLFGANPLTGSLGVVTLNLARIGYVAKSKEEFLERVYKLMDIARDSLEIKRK
                     IIEKFSDDGLYPYSTHYLADIKARFGAYWQNHFNTIGINGMNEALVNLLGKDITTPEG
                     HAFALEVMDAMRERLMDYQNETNNLYNLEATPGEGSTYRFAKKDLEIYPDIHFANTEA
                     VKNGAAPYYTNSTHLPVGFSDDIFAALDLQDDLQVKYTGGTVLHGYLGERLPDGNAAK
                     KLVQRIINNYRLPYFTITPTFSICPKHGYLSGEHKYCPKCDEEMGFTSADMQAERCSD
                     C"
     gene            24419..24562
                     /gene="nrdD"
                     /locus_tag="PKK65_01125"
     CDS             24419..24562
                     /gene="nrdD"
                     /locus_tag="PKK65_01125"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008339701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HNU81321.1"
                     /translation="MSPITTQRQECIVYSRVVGWLTPVKSYNPGKTSEYKDRKTFKIT
                     CSE"
     gene            24629..25360
                     /locus_tag="PKK65_01130"
     CDS             24629..25360
                     /locus_tag="PKK65_01130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HNU81322.1"
                     /translation="MLIGGLEKLSLLDYPNNLAAIVFTQGCNFRCHFCYNPMLVWPRS
                     EKLAPDEKDKDKGYPLIQEDDLFLFLQKRLGKIDGVVITGGEPTLHADLPSFIKKIKA
                     MGYLVKLDTNGTNSLMLETLFSEKLVDYIAMDIKAPWPKYEKVVGVPVNLENLQKSVK
                     IIMSSGLPYEFRSTLLPDLHDANDVEDMGKMIQGAERWYLQKFKSDTNLVNVDFEGKN
                     TFLDKELKELALIGSKFVKECRARV"
     gene            25365..25646
                     /locus_tag="PKK65_01135"
     CDS             25365..25646
                     /locus_tag="PKK65_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81323.1"
                     /translation="MDDLPKEVDSLASSESVKTEDLALLLKENLEMSKEIRAMVRHIN
                     NYVAWQRIFGWIKVLLIVIPLIIGILYLPPFLKGLLSQYESLIGGGLGS"
     gene            25667..27598
                     /locus_tag="PKK65_01140"
     CDS             25667..27598
                     /locus_tag="PKK65_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HNU81324.1"
                     /translation="MDQEIAVFAQTTFRNQFKKFGIKTDDRRRHMYLIGKTGMGKSTI
                     QENMIVGDIRAGHGLAVVDPHGDLAEKIIEYIPSNRIQDVVYFNPSDIDYPIAFNVLE
                     QVDPHLRHLVASGLLGVFQKLWADSWGPRLEYILRNTILAILDFPGSTLLGVVRMLSD
                     KSYRKRVVANIKDPVVKAFWEKEFASYADKFASEAVSPIQNKVGQFLSSSLMRNIIGQ
                     VRSSFNIREIMDQGKILIMNLSKGRIGEDNSALLGAMMITKIQLAAMSRVDVPEKERK
                     DFYLYIDEFQNFSTDSFANILSEARKYHLNLILAHQYIEQLSEKVKPAVFGNVGTIIA
                     FRVGAADAEELAKEFAPVFTEEDLVNLPKYEMYLKLMIDGVASSPFSAKGLPPLSDEE
                     KTNNVDQVIAYSRKKYASSRAEVEEKILRWHEAQDDIPSTPIRPVAPASPLFAMSPKS
                     LEAKTGVSAINQPTIARQSAEKNIERGKAIPSSSPKLQERLIFQGTNTAVAPIKQAGA
                     SQSSAPVFGIRAVKNPSAAKGYHAICSRCGAETTTVFVPDGIRPVFCKDCLSLKRQER
                     KREEDNRRQAKAEELQAFKNIPNPSPSLSLSSLRQARPVDFRGRPEKIVPPIKKNDFQ
                     EEGQDMAEDELIDFSRRPE"
     gene            27603..28178
                     /locus_tag="PKK65_01145"
     CDS             27603..28178
                     /locus_tag="PKK65_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006366356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNU81325.1"
                     /translation="MYLVFDTETTGLPKNYKAPISDLDNWPRLVQLSWAWFDAAGKAW
                     DRYDYIIRPDGFIIPEEATKIHRISQAQAVAEGKDLKEVLLEFAEHIARAEALVGHNI
                     DFDDKIIGSEFYRNQIPNELAEVKKICTMKAGADICRISDGHGGYKWPNLSELHRHLF
                     NRDFTEAHNAMVDVLACARCFFELRQRGFLI"
     gene            28181..29761
                     /locus_tag="PKK65_01150"
     CDS             28181..29761
                     /locus_tag="PKK65_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNU81326.1"
                     /translation="MNYLKIGKATDLQGKDRRLYRFFEILPGLLSFSTLLILLIFSYF
                     QPVWVAFFIIAFDVYWLLLVIYLALYLVAAYRKLKKNTAINWRTRCEDLNRGDFSDPK
                     ISPDALARQGKHWQDLWHLLIFPNYNESEDIIASALESVLQDDFPAEKFIIVLAMEER
                     AGASAHERAIAIEKKFKHRFGRFFVTFHPDGIEGELKGKGANQAWAIKRVKEELLDVE
                     NFDYDNILVSVFDIDTMVRPGYFFALSYKFLTVSSPYRASYQPIPVYHNNLWEAPFFS
                     RVAASSNTFWQMMMQIREEQLVTYSSHSMTLRALVDIGFWSTNMVSEDSRIFWHCLLY
                     YGGDYRVEPLYFPVSMDITFDRNFWQTARSLYKQQRRWAWGAENVPYLIFNTIKSWHR
                     IDRRKLISHIFIQLYGFHSWATNALIIAVIGWMPMLLGGARFNSTVLSGNLPAITQSL
                     MNIAMIGLVLSAIISTLLLPKRPPKYTVFKNVRMVLEWIFVPFTIIFFGAIPCLDAQW
                     RLLRGKYMGFWVTPKSRN"
     gene            29815..30237
                     /locus_tag="PKK65_01155"
     CDS             29815..30237
                     /locus_tag="PKK65_01155"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNU81327.1"
                     /translation="MTKQKMISNLKASRRRETNFLYRFLVNPRTFAIIALVFLLLIFL
                     PLAKNYSRKRLVEQEIAGIQKEIDDFEAKNRDLKEMIDYLQSDQSLEEQARLNMGLKR
                     PGEIVAVIQEDTAVNAISSPVLVDDSPNWQKWWRYFAN"
     gene            complement(30292..30364)
                     /locus_tag="PKK65_01160"
     tRNA            complement(30292..30364)
                     /locus_tag="PKK65_01160"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30329..30331),aa:Thr,seq:cgt)
     gene            complement(30372..30443)
                     /locus_tag="PKK65_01165"
     tRNA            complement(30372..30443)
                     /locus_tag="PKK65_01165"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30408..30410),aa:Gly,seq:ccc)
     gene            30509..32128
                     /locus_tag="PKK65_01170"
     CDS             30509..32128
                     /locus_tag="PKK65_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlpA-like double-psi beta-barrel
                     domain-containing protein"
                     /protein_id="HNU81328.1"
                     /translation="MKKRYFLSIFISLSLAAGFLLFKSSPVSAYEAYVGITPPADTID
                     SVDEFNKKTLQENLVAGQERLLRLESFSVKVPAEALSKNAVLRLDNLGEDFPLPWNYE
                     LLGHVYQFDFASTSQHYNQKVFLDLSIDYGEKNNYYKQIFFFDGSSHRWRPLPSSDDP
                     SNHRISAKINFPFARVAVLYNDQVLTVGQASWYRFQGGLYAASPDFKKGTVLKVTNLD
                     NNKSVSVTVNDYGPDRSLFSNRVIDLDARAFEKIASLETGIINVKIETEKIIAPEDSQ
                     KLQAASSDLNLSSQSAVVLRESDGKILYDKNATSVSPLASLSKLVAAQVFLDTNPDLN
                     KVVSYSVQDENYNYQYCKPWESARLTVKEGDTLTIGDLLYSALVGSANNAVESLVRVS
                     GLSRSEFIKKMNDKVKSWGANNTHFVEPTGLSPDNVSSPLDYAIITRELFKNSLLQKI
                     STTARYSFSTINTKVRHNLNNTNNLLLNNNYKIIGSKTGYLDEAGYCLMTRVDSAAGR
                     LIVVNFGAKSKSDNFMDNELLINYGLKKLSQ"
     gene            32135..32815
                     /gene="ftsE"
                     /locus_tag="PKK65_01175"
     CDS             32135..32815
                     /gene="ftsE"
                     /locus_tag="PKK65_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HNU81329.1"
                     /translation="MISLLHVSKSYSRRNKILSDVNLHIKAGEFVSVVGQSGAGKTTL
                     VKLLIGEERADEGQVVIGDWDITRLNRHEIPYLRRQIGVIFQDFKLLPKKTLRENVAF
                     ALEVCGSSQAKIKKIVPSVLKIVGLEDKGDRYPHEVSGGEQQRAAIARALVHQPRILL
                     ADEPTGNLDAINAEEIMELLLKINKFGTTVILVTHNKEIVNRLNRRVITMDKGRVVSD
                     QAHGKYLL"
     gene            32829..33737
                     /locus_tag="PKK65_01180"
     CDS             32829..33737
                     /locus_tag="PKK65_01180"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNU81330.1"
                     /translation="MLSFLRIIKFSFQDIVRNIWLSIVTITILLLALFSINTLLTVRV
                     VSDNAVNAVKEKIDISLYLKPEATESDIAALRSRLSSMPEIKAVNYTSKQAALNSFRN
                     KYRDNPEILQALRELGNNPLSPSLAITPANFDDTENLVNKLRQLDSDIIESRDFSNNT
                     AVVAKIQAITNRINEIGLVIIAIFVLTSLLVVYNSIRVAVYTHRKEIEIMRLVGASNF
                     FIYMPYFFSAFFYALMSVFIMIIIFYPFLTLFQPYLEVFLAGYNVNLLSYFVYNFWPI
                     FGAQFGVIFIITLIASFFAVRKYARV"
BASE COUNT         8811 a         6302 c         7587 g        11143 t
ORIGIN      
        1 accggcacaa ctaccattcc taatgttaat aagggcgaga tgagtagttt tgtggtggct
       61 agtattgctg gtaataatga aaaaagcaat tatgttcgtt ttgtaaagca ggctgagccc
      121 aatcagggtc cttatattag ttattttgag ccaagtagcg gccgtgccgg tcaatatgtc
      181 accatccatg gctctggttt cggcggcgct cgtggatctt cccgggtatt ttttggggac
      241 aaggaggcca gttatgattt tcccgaggtt tgcgctagtt ctgtctggaa agacaatcaa
      301 gttatcgtga aagttccggc cgatatcaat gacggcaatt atgtgctcag gatggaaatt
      361 aatgatgata aaattgattc tcaaaatgcc aaccctaatg tttttacggt tgatgctaat
      421 tcttctttga agactagtat ttgtcgtatt tttcctacgc gtggtcaaat tggcacgccc
      481 gtagaaattt ggggtgaata ttttggcgat aacggtggca atgctttaac tgttttttct
      541 tataaccgtt cggtcagcgg tcaaattagt cttgaacaat cagctcagaa attaaatccc
      601 agcgtgcctg ataatgcagt gaccggtgcc gtgaaagtgg tcaagaatag tgaatggggt
      661 aatgatgtta attttgaaat tggtacttgc gttgataata gtgattgtag tggcgatact
      721 tgttgtccgg caggcaccta caagcaagga cgttgcgcta cttctttggc cgagtgttat
      781 gtcgacattc ctaattccgt gtttgaatgg aatttttcta ctgagtttgg cggggttgag
      841 ggtgatgatg agtttgatag ttgccaagga atggctcagg ccttgggggc ttgccaggtt
      901 ggcgcttttt gtcctaattc tccgggttta tgctcccctt atgccggtgg caccaaggtt
      961 ttaggttctt gcgattccag ctgcgcgact gtacctgctt gtgctgagct aggcgaagga
     1021 acttgtgttt atgatcatca atctgatcga tgctttatta ataataatac ttgttcccta
     1081 gattctaata ttagttatac tcttggtcag caaacattta acgctgttca atcttgtaag
     1141 atttttagcc aatttggcgg tcaagctcat tggcaaataa gcgttgcctc ttcttgtcct
     1201 aatggttgga cacgtcttga tggcggtcat tgtgtcgaca gcacgagctc aacggagtca
     1261 acttgttctc tctgcccgac aaactttaat tgtcggccaa tcggccaaga aggacgttgt
     1321 gtaagtgccg agttatgccc cggtagcgcc agttgtgatg gcacttcttg tttagctgac
     1381 acttcggctc gttgcgattg ttgttgtgaa atcggtcagg atgctcgtga ttgttgtttg
     1441 cctttaactt gtagtgggac ttgtggcagt gatactagcg acgataaagc tggtttaggc
     1501 caatgttctg gttgtatgat cgatagcgat ggtaatggta ctatttctcc ggaagagcaa
     1561 ttattatctg atcaagcttg taattgtagt gcccattctg gtaaatattg tgatatttcc
     1621 gtgccgactg gagcttgtgt tgactgcagt cgcctatcag tccagtcttg tttaaatcat
     1681 agtcaatctt gctgtcttga ttcaaaaggc accgcctccc ccgcagacga tgtttgccgg
     1741 ggtggtaatg gtcaggaggt gagtgccgat cctgccagcc ccgatttcgg ttattgcgcc
     1801 tattatgatt gccagaacgc ttcctctact cctcctggtg atccgagttt atgtgcctct
     1861 tcgacgccta aggtttttgg attatttaag aacgtggaag catgcgttaa taattgcgct
     1921 gctagtcccg gtttatcttt ttgtgggcaa ttcgacggcg atttggttgc ttgtgcggcg
     1981 gccaacggtt gttgttttaa ctttaatgac caaaagtgca ccagcggcgc gcagatgctt
     2041 ggtggttatt gcgcctatta taattgtcag gcggctccta atgaggcgca gtgtgacccc
     2101 aatccttcgc caataggaat ttataacagt cagacggctt gcagtattgg ctgtgctcag
     2161 ccaccatttg gcggtctagg taaagattgt cgcaacttaa cgactaataa cactgactgc
     2221 aaccagtctt tttgttctcc tcctttcgct tgtcttaatg gttccggtaa tattggtctc
     2281 cccggcgact gtggcgcttg ttgctgccaa gtaggtaatc ctgattcttg ctccggcatt
     2341 ggcaatggta gcttagtttg tcaatctaac cagacgcctt gttccggcga ggaccgcggt
     2401 ctttgctgtg gttgcagtca ggatgttgat tgtggcaatg ctaatacttt aggctgtgac
     2461 gccagcgctt gttgtcgtaa tcgtccttcg gtgttaggcg accagttggt gccgcctcat
     2521 ggcgctaccg gtgtttgtcg caatgccagc atcagagtgc cttttgatca gttaatggac
     2581 gtgggaagcg tgatggataa tattttatta ttagaagagc atgattacgg taatggcgtt
     2641 tgtccggctg gtactttctt agcttctgta agcaccgtca acaccatcaa ccataattgg
     2701 ttagctaaag cttactctca agttaaatcg tctttccggt caattttaaa tcgattaggc
     2761 ttatcttctg ctcctgtttt agccttaaat ccgccggcgc ctgataaact ctattgctcg
     2821 attccaggta atatttcggt tattaataat ggtaatggca gcgtggctga atttatccct
     2881 aaaaaattgc tatctccggc tactaattat tatttaattg ttaaaggcga cgaatctttg
     2941 gatagtaacg ctggcgtttt aagccaatgg caaatcggtc ttaatggccc cggatattta
     3001 aatttgggga cgaacagtta tattgaaggc gaaaatatta aatttaataa tttaactttt
     3061 aaaaattctt atattgccgg atttaccacc ttgccccagc aaggcgctaa tgtcggtatc
     3121 tgcgctgttg actatgtagt taccgaaccg gcttcttatc tttttcaaag taccgataat
     3181 gaccttaatg aagacgactc caatcctaat agtaatagtt tcgacactaa aaatgaccgt
     3241 gataaattat ttaaagctgg tgcttatagc gctgatgatc agcttttaca tcctagctcc
     3301 ggctattatt gggattggtc ttggcaggtt gctaaaccca gtattgccag cattgcctat
     3361 gttagcggct tagccgataa tcaagctttt gtggtggccg cagacggtgt ttccgatgat
     3421 tcgacgattc tttctgctca aattgatatg aatcgatttt cggcgcctaa ttgtaacagt
     3481 tcagcttctt gtgtttgtgc cgaaccggaa tgtgtgaata attgttgtaa tatctatcaa
     3541 gagggggacg gcttgcaagc tgagacgcct ctatttatct tcctgtgtaa aaatccttgg
     3601 ccagcagtta atccggttac tttagcctgg tcgccgtggt atgatacttg tgtcggcgct
     3661 attggtaata actgctctaa ttataattat aaattttatt attgccgcga tgccggcgct
     3721 gatggcctgg ctgatgattt gccggcaatg attaatccgg ctgtaattcg cggtactagt
     3781 aattccttga tttgcagtga ggggcaaacc gcttgttcta acttaggcac aacttgcggt
     3841 cctgataata attatgatgg cgttggcgac ggcttttgta tttggaatat tttaaaggag
     3901 tcatattttt tcaaggaggc cacgccccaa attggcgcga ttaccgccgc tattgatcag
     3961 gaagacggcg attcggttcg cttagagtgg tatggcgacg ccagtgtaat ttataatgct
     4021 aatgccagtt taatgggcaa gtatcgttta tattatgccc cggtctcttc cggcaacatg
     4081 tcttttgttg atattagtcc cagggatgct tatattgttg gcgttcccgg tcctgtttgt
     4141 actcccctaa cccctgccaa tggagaaaat tattcttgtc attatttatt aaaaggattg
     4201 caagcaggtg aaaattatcg ttttcgtata tcggctattt cctcggccca agtagagagt
     4261 cctttatctg aagagaagac ggtgttaatt accgatactt tagcgcccgc acctcctcag
     4321 ggcttgacgg ctactattgt gaacggacag cgattacatt ttgtatggca ggcgaatacg
     4381 gatgatactt ctttttatcg tctttaccac ggtgttagca gtggccaata tggcgaatct
     4441 tatgattccg ataaacaggc gacgaccatg gatttgaatt tgcagcaatt tagtcaaggt
     4501 attcattatt ttgctttgtc agctttggac tcttctaaca atgaaagcaa taaatcggcg
     4561 cagataacca tcgttgtccc cgcgccctaa ataaatttat atgaacaagc gaggctattt
     4621 ttacgcttta ataattgtag gtttattttt ctcttgggct cattgcggcc aagctgctaa
     4681 atctaccggt gacgagccgg ggggacagac tattaataat actattaata atacttttat
     4741 tgatgacaag gacagtgacg ttattgcggt gagagtatta cctaacccta atcattatag
     4801 cgccgctcgt tggtataaga gtcagggatt ttctggttct ccgcaggctt tattggttga
     4861 tggttatgat gctgtccgcg acggtcgcac tgtttatgta aacgccgcga atgttgattt
     4921 ggctactaaa aacatctata ccaatatcta tttgatttct tataatcaga atccccagct
     4981 aaaaacagtg gatgtgttgg gtcaaattat tagccactgg cgctttaaca gcaatttaac
     5041 tacggccggt aattgttata tttccacttt gctttgtgaa aatgacagtg attgtagtca
     5101 gggacaatat tgttttggta aaaccgaagc gcccggacgt tgtcgacctc aagaaaactc
     5161 tgtttgttat accgacgccg actgtactca aggattatat tgtgatagtt tgcgcgccaa
     5221 ggtggctaga gatgtgaggc gtttgggtat gcttggtgat ttgcgtgaat ctctgtccgc
     5281 ttttaaaaaa attaataaca agtatccgat tcttggcgct ggcacctatt tgcctttaag
     5341 cagtatttcc gtttggccta gttggcaatc tctcttttta ccccaattag gaatgcccca
     5401 gtcattaatt gatcctatta ataagcttgg tccttgcgct ggttataata gtgaaacctg
     5461 ttggaatgag gaaaccaaag cctttgctga tccaaaccct caggataata atttggaatt
     5521 accaggcggc agctatgctt ttgtttattc cggggatagt aatggttcga attatactct
     5581 ttgtgcggcc atggaaacaa agagtttggg ctacaacacc accgaggggc aactggcgga
     5641 atcaggttgt gtttctagtg gcgccggtta tattggttct ggcaataatt tggcgccggt
     5701 tcttatcgcg accaacctgc aaggacagca agatcaggaa tttaatggat ttattaaagt
     5761 aatcgaccct gatggtaatc cgatggattg gtctattaat acagcggcca ctgtttggac
     5821 taattggcgc aataatggcc agaataatgt cgctcccatc atgcttgata cctctaatcc
     5881 tcatcagaag aaaatctatg cgcaggcggc cggtaaccct ggcacctata atctgtcttt
     5941 atcggttgcc gatggccagg gcggcgtgtt gtcgacagtc acgccgatta ctattgttaa
     6001 tactgctcct ttaatccagt cagacgatat ttattattat ccgagcaccg tcattccttt
     6061 aactattaat tttagtctta gcgataaaga tcagcccatt tcttatacta ttacgaaggc
     6121 gacctggaat tccggcccct atgatttatt agcgccggct aacgctacat ttttatcgga
     6181 aagtcatcat caggtcggcg atactgttta ttacaccctt aagtataatc ttcatactag
     6241 taataaattt acacatgata ctacttttgt ctacgtcatt acggctaaag ataaatataa
     6301 taataccgcc accagacaat taaacattaa tatcaagatt gatccgccgg ttttagattt
     6361 caattgtgct tcttcggtga gggttggttc ttattattct tgccggctgg gatttagcaa
     6421 acaaggcgat cataccatca cttatagggc cgtcggttct ttgcccagcg gtttaagtat
     6481 tggagaagtg atgcaacccg gtgggggtgt tggcggaggg agtattcagg catcgcgagg
     6541 ttctttgtgg cggtttattt ctaaattgtc ttcctggttt aaagccgcag aggcggcggt
     6601 tgctaatacc tcctacgctc tgcagggcac gccgacaacg gccgcgtcgg ctgtgtccat
     6661 taagattaag gcggaaaatg aatttggcgc tgtttccgaa agagaatttt tacttaatat
     6721 caataattat tgtggtgatg gtgtgcgtca ggctccaaat tcagaggggc ggggaggatt
     6781 ttataacgat ggtcatgagg attgcgacgg caatagcggt attatttcag atcgttcgca
     6841 gattggtagc acctcaccta cattacagta tggctgtact actaagctcg gcagtgctgt
     6901 gccttacccg ataatgaata catattattg tatttatctt tccgctgatg accccaacgg
     6961 tggcggctac tgcggtgatg gtatttgtca agcgcggatt attcgcaata atcaacttac
     7021 accttgggaa ataggtggtt attgtctcga agactgtact tgtgctggcg accatctggt
     7081 gccagtcggc gatagctgcg agtgcgaaac aggttggtat gattgtgata gtgaggcttt
     7141 gggctgtgaa agtgagaaac cctgcgacgg ttgtggggct gatcagtatg aatgtggaaa
     7201 tcaatgtttt tcgaaaaact cttttgactg tggcttatat tcgtctaagt gttacagtaa
     7261 ttccggcact tcttgcacta ctagttgtcc tagtggttat tataattgta acggcactaa
     7321 tgagtgtgag agcacttctt gcgtctgtcc agatggacaa cattgggaag ctaatgtttg
     7381 gcctgatggc gcgtgtgttg ccaatactcc cgccggttgt cctaatggga tgttgcagtg
     7441 tggcaccacc ggtttaaatt gctataaccc tgacacccag cagtgttgca gcaatagcac
     7501 tgtctgcggc atcaatcaat attgttgtaa tgttagtagt cagattctaa cttgtgtcaa
     7561 taattatgtt acgtgttata ttatcatgtc aggtggtaat agtggggctt ctaattaatt
     7621 attttttaag gtacctataa attaaagcaa gccctttaga tcccccaata aaataggggt
     7681 tggaaatata atagagccag ttaaaatatt aacaattcat atttaaggca tcagtcaaac
     7741 aacggtagga cacttttaat aagtatcact atcgccgtaa cgattttcaa tcagttacct
     7801 atgcgagctc tattaccagt aggctcaagt gtgctgagac gataaggatc tctgaattat
     7861 gaaaacgatc ttaaaggatc atatctttaa gcagagtatc ctatcgttgc ttgactgatg
     7921 caaattcccg aaagatagtt tggtcagtta acatcgcaaa aataattgaa cataattttc
     7981 tggccgtcgc caccttggct ttccccgtcc ccttgttctt cttgagtttt cggtagaagc
     8041 tggccaagcg gttggcgtgt cgacagttag agctaacggc gttaatcaat gtcttccgga
     8101 gccaaattga accccgtttc gtgatccgtc cgtgtctgat tttatcggcc gaagcgtaag
     8161 tcgaaggaat gagaccagca taggagcata atttattagc actagagaat ctggatatat
     8221 ctccgatttc tgatttaata accagcgccg agaaaaaact aatacccgga atagtcatca
     8281 gtagtttaca gtcataatcc gctttggccg tattttcaat attatccgat aattccttaa
     8341 tttgtttccc taaataatcc agcgtgagaa gatatcgatt cagctcatct ctaaaaatcg
     8401 gtcttagttc caaggccttg agccagttca ttcccgctac cccgtataaa ttagtatatt
     8461 cgtgtttaga gttagtttta aacagcaccg aatggatttt gttcttgatt ctcgtttgca
     8521 tttccacgta ttgtatccgt tgtctaacca gttcttttag atcccgtata tctctaggtt
     8581 gaatataggc gaggggcaac atcttcgtcc gaagcaaatc agctaaaatt cgagagtcca
     8641 ccttatcgtt tttcattttc gagaaagcaa tcgctttcgt ggccaaggga ttggacaata
     8701 ccgtctggca gcccaatgat tccaggttct catagaaggg tagccaattc atcgttgact
     8761 ctaccgccac cagattattg ccggcatagc gagaaaagaa cttcgccact ttatccgtat
     8821 cggtggagat ttccccttgt ttcttaatct taccagtcaa gttcattacc gttatatagg
     8881 ccgtcttctt atgcaaatca actccaatat agttttgata actagtggtc atatttttca
     8941 cctcctctcc agctacattt tatctttcta gtttaacact tttatatata ttaatttata
     9001 gcattatgaa aaaaaatagt attataatta tcggagcggt tatttgttta atagtagtgt
     9061 ttgtcttact gtattttttc cgggatgagc cgtcaacaca gacccctttg ttgaacgacg
     9121 tccagcctca gtcggtagcg ccggtattaa tgacagcaga ggaaaaaaac agccttttta
     9181 ttccggtcga taaagaagtt caagtgttac gtcgtaatgc cgacggcgaa attatgactt
     9241 acaagattat tgaaagccaa gatcaaatta taaataatgg ttcattattt ccttctaatt
     9301 cctcagaact taagtaggga tgcatttttt tagattataa gttataatat aatcaagggt
     9361 ttttctataa aaagggtttt tttataaaaa ataatgagaa aattatgttt aacgatttaa
     9421 acaaacaagt tccggctagc aatgctgttg atgatatttt tgccgaaact gaatctacag
     9481 ttcgctcatc cgaacaaaaa gtaaatattg aagctcaatc ggctggctta tcggctccat
     9541 ttgcgggaga agataagtct ggggataatt cttccggggt aaaaataaaa tttatattga
     9601 ttataatttt agccctagta attttagccg ctgccgccta tttagtttat gcaaaaatta
     9661 cgcgcaatgc cgctgatcaa gctttagata gtcctgtttt aaataataat gttgcgccaa
     9721 ttgtaaaaac cgataatagt gtcggtactg tagttatgcc cgaggtgcaa agcccagaag
     9781 taaacgctac cgctaccacg tcagatattt ctccagtcgt tacgcctaat attatttcgg
     9841 caacagaaac cccggtggca gtcactgccc cggttgattc tgatggcgat agtttgacgg
     9901 acgcagagga aacaattctg ggcaccaaca ttaacctaat cgattccgat tttgatggtc
     9961 tatctgatta tgaggaattg agagtttacg gcaccaatcc tttaaatccc gataccgacg
    10021 gtgatagtta tcatgacggc gatgaagtaa aaaatggcta taaccctaaa ggtgtgggcg
    10081 ctctgtaacc ccgcttcgta tctgctcatt ttatggctct tgctaatcaa aaattaaata
    10141 aaaaagattc taccgttggc ggtgtacgtt tatatttacg cattcatgtt atgcctgtat
    10201 taaataagcc cttgcgcggt cgggcttggc aatggattag cccttttgtc gctctggcta
    10261 ttttgattgt tgctatcttt tattttttag ctgattaata tggatagtaa gaataaaaaa
    10321 atgccaaata aagatgacca ggaattacaa gagcgtttgc aaaaagactt tattgttcgt
    10381 aatatgccag cgcttactag tttgagtggc gctagttatg gcgataaaga agaaaaaggt
    10441 gttaatcatc gtcaggctgg ggataacggt ggcaagccta aggataataa gcatcagtca
    10501 acgggaataa ttattattag tgctggatta atagtagcgg caggtttatt ttatgccgct
    10561 tatcattttt taattttgcc ggcaatgcga ccatcggttg ccgttaatac cgctccggtg
    10621 gctgaagtta ctgagtcgtc cgtcgctgaa gaagaagaaa aggcgcaaac agccacaatt
    10681 gtgcccgaga cgatggttgc tccagtggta gaagagctgg taaatagtgt tgaaccagat
    10741 agccaagata gcaatattgt ctcgttacca atcgtagtcg attctgataa tgacggtctt
    10801 agcgatgttt ctgaggtttt tttgggtact aatcctcaat tagctgattc cgattctgat
    10861 tcttatcctg acaaacaaga aataatcgct ggctacaatc ctctcggcgg tggtaaattg
    10921 tctgacaacg gtttttctat ttatgttgat ccgaatggtt tttttgttga tgtttatccg
    10981 cagaattgga cggttaatat cgtcaatcaa aattccgttt tatttgccgc tcctgaccaa
    11041 tcttttattc aaattgcccg cgaggaaagt gatcagcctt atgataacat tctcgcttgg
    11101 tacagcagtc agttttccga tgcctctact ttggatgtca gccgtttctt ggagggcaat
    11161 atggggattg gtattattag tgctgaccag caaatagttt attttttaga ccgtgacaga
    11221 tctcatattt tcgtggtttc ttatattaaa tcaactgagt ccatgcctta tctggaaatt
    11281 tttaagacga tggccgctgt tttaatgttt ccttaaatca ttataaggtc aggaaatctt
    11341 atataaaaaa gccatcccta agcaggcggt ggctttttaa atgcggggag gttattaaga
    11401 ggaggtttct attctttaga aattatttta tctatcaaac cgtatctttt cgcttcttcg
    11461 gcgctcataa aattatcgcg gtcactgtcc ttttcaataa tatttatttt ttggccggta
    11521 tgttgagcca atattttatt cattttgtct tttatcttta aaatgtgctc agctctgatt
    11581 ttaatatctg aagcttggcc ttcggctcct cccattactt gatgaatcat aatttcacta
    11641 tttggtaaag ccagtcgctt tcctttggcg ccagccgcta agagaataga ggccatcgag
    11701 gcggccaggc cgacgcaaat agttgatacc ggacatttta tatattgcat ggtatcatag
    11761 atggctaaac cggcagagac agagcccccg ggggaattaa tgtaaaattt tatatctttg
    11821 tctttatcct cggctgccaa gaagagcatc tgagcgatga cgatattagc cacatgatcg
    11881 tcgatttcct cgccaagaaa aattatgcgg tctttaagga ggcgggaata gatgtcataa
    11941 gccctttcca cttgtccttc tttttcaata acggttggta ttaaggtcat aaagttaatt
    12001 tatattattt ttgctattat taaaaattat atggcagaca tcttcttttg taaagccttg
    12061 tcttgatttt ttcgtgtttt tagtctaaga ttttaaaagt ttctaatctt tggcaataaa
    12121 ttaaccaaaa tctatgtctc aaacgcaaaa gttttctaat tttatgaaac gccatcacca
    12181 acgctggttg tttttattag tagtagtttt tgcttttctc ggatttatat tagcggctgg
    12241 caattactat aatcgcggtg ttcaggcttt ggctaatgcc accaataata ttatcgtttt
    12301 accaaaggcg ccagacagtc cctttgttct cggtttagat ttacaagggg ggacacaatt
    12361 ggtatatgaa gccgatgttg cccatatcgc tgaaggcgat cgtctcaatt ctcttgaggg
    12421 tgtgcgggac attattgaac ggcgggttaa cagcgctggc gtcagtgagc cgatagtcca
    12481 gattaatcgc actttgcagg gtgattatag agttattgta gagttagcgg gtattaagga
    12541 tgttaatgaa gccattaagt taattgggga aacgccgtta ttggaattta aagaacaaag
    12601 tacagaggcg caagcagcga cggtcgaacc taatgcagaa gccgttgcct ttaacaaaca
    12661 agtggaaatt aaagcccagg ctgctttaaa tgattggaaa tcaagtagtg attggcaggc
    12721 aatagcggat aaatatcaag ctaagaatga gataaaaaat gatattaccg ctgacaatga
    12781 gccagcttta gcggcggctt tgcaaaatct agcgcctaat actattgttg cttctccaat
    12841 taatgaaggg cagtctttga ttattgctaa gcttattgac aagaaagaaa tagacaatcc
    12901 ttttaaggat gaatctgggc aagagccggt tattacttca tattcggcgc aattgttgtc
    12961 ttttccgctt caggatgaat cacccgttct gagtttaggt gataattgga aaaatactga
    13021 actttcaggt agaaatttaa agagggcaac cttgcaattt aatcctcaag ataattctcc
    13081 ggaagtagct ctagaatttg atagtgaggg cggaaaaatg tttgcagaca ttactgagcg
    13141 taatatcggc aaaccagtgg ccatcttttt agacggttat gctatcagtg tgcccactgt
    13201 taacgatcgt atccccgatg gccgtgccgt tatttccggt cattttaacg tccaggaagc
    13261 taagttattg gtgcaacgtt taaataccgg cgctttgcct gtgccgatta atttaatcgg
    13321 tcagcagacc attggcgcca gtcttggtca gaaatcaatc agtaatagtg taaaagccgg
    13381 tttaattggg tttactttag tggctttgtt tatgattatt ttctatcgct tacccggttt
    13441 aatgtccgtt atttctttag gtatttacgt tttaacagtt ttaaccatct ttaagtcgac
    13501 gccgatttgg ttggccttat tatttattct cttgctggtt ctattattca tcagtgtctt
    13561 taatcattta aagatatttg atggcgtctt ttcggcttta cttttcttgg tcattctcgc
    13621 tttcttggtg ttttatgcca accaaccggt gactttgact ttagcaggta ttaccggttt
    13681 catcttgtct atcggtatgg ccgttgatgc taatgtgttg atttttgaac gcatgaaaga
    13741 ggaattgcgt tctggtaaac ctttggcggc ggctctagat gatggcttta agcgcgcttg
    13801 gccttcaatt agagacggta atattaccac tattttgact tgttttatct tgatggggtt
    13861 tggtaccggt ctggttaagg gttttggcgc cactctgttt attggtgttt ccatctccat
    13921 gtttagcagt attgttatta cttatatttt attaaaaatg tttaaaagct cttggttgga
    13981 taagcatcgc tggctgttag gttttactcg cactaagtaa taaagtaggg gcggcattat
    14041 ttatgcgctc tgtctgaata tatgaattta caaatcatta aacatcgtaa tctttggttg
    14101 tcactatctg gaattatggt agttgcctcc attattttta tttccctttg ggggttgaaa
    14161 ttcggtcttg attttactgg cggtagctta ttggaggtaa aatttaccag cggtcaaccg
    14221 tccgtttcta cggtccaagc tggtttaaat gacacccaag tcagcagtct gattatccag
    14281 ccaactgaag attcaatgat tcttcgtttt caagaaagtg atgaagatac tcatcaggcg
    14341 gttgtaaacg ctttaagaca gttaccggag gctcaagccg gcttggaaga agtacgcttt
    14401 gaagccattg gcccttcaat tggccaggaa ttgcgcagta aagccttttg gttgatgttt
    14461 tttgtcttaa tcgttatcat tatctatatc ggtttggcct tccagcgcgt ttccaagccg
    14521 gtagcttctt ggaaatatgg gctgacagcc attattgccc tttttcatga tttgttgatt
    14581 actatcggtg ttttttcttt gctcggtcat ttttacggcg tagaaattaa tactaccttc
    14641 gttgtcgccc ttttaaccgt gcttggttat agcgttaatg acactattgt cgtgtttgat
    14701 cgaacccgtg agaatttgcc taagagtacc ggcagttttg ccgagaccgt taattctagt
    14761 ttgaatcaga ctctcgtgcg ctctattaac acggtaatca ctactttatt ggctttattg
    14821 gcagttttat tctttggcgg cgattctatt cgtgaatttg ttctagcttt aattattggt
    14881 attttttgtg gcgcttacag ttctatcttt gtggtcagcc ccctcttggt tgtttggcag
    14941 cgcttacaga aacgctaaat ttaattatta cttacaaaac tccaagctcg caatggtttg
    15001 gagttttttt atatgttata ctaaaagtag ataatttatt tgctttatgg acaggaagag
    15061 tttgaatatc aagagaatat tatcattctt tttgcctagt ttttattggc tggctttatt
    15121 ggcggtcccc ttgagcgttt cttatttttt tcctatagcc agtccttttg tcttaataaa
    15181 gagcgcttgg ttacaaattt tagccgccct cactttgtta gtttttttgt tttttgtagt
    15241 ttttagaaac ggtcagagcc aagtattttt caaggaaaaa tcttggtggc gcgctatttt
    15301 accggtgtgg attttttggc tggctttatc cgttttatct ttctggtcgc ttaatccttg
    15361 gcagtcttgg tttggttcat atctgcgaca aactggtatt ttattttatt tttggctggc
    15421 tatttggtat agttttataa tttattattt tggtggtttt tataggccgg aaaatagggt
    15481 tgttttttgg ctgcgcggag tgcgccttag cgctttatta atgtcaattg ccggcgcttt
    15541 tgccggtctt tatgcctttt tgcagttttg tgggtatgat ttagtggttt ggcaagaatc
    15601 tcagctttat agtcgcgcca ttggtacctt gggtcagcct aattttttag gctccttttt
    15661 acttttttct ttggccatgg ccgcttatta tttttttgat tctaaacggt ttttaaataa
    15721 atttttagct ttactgatgt ttctcttgca attagccggt ttagttgtta gcgcttctcg
    15781 agcggcctgg ttggcgttaa ttgtgtctac tattattttg ctggtgttgt ttgcgtggca
    15841 acgttggcgc tggcgtgctc tggtggttgc cccgatttta ttatggtttt tttcgacttt
    15901 tttttactta ttaatgcccg cccgttttag ttctttaacg gattttaatt ccggtagcct
    15961 ggctttgcgt ttttctttct accgtgcatc tttccagatt ataaaggaaa atccttggtt
    16021 tggggtcggt tgggaaaatg gcgaggaggc ttttattacc cattatcagc ctgaatgggg
    16081 ggttttaatg aatgtagatt cgttgactga taaagcccac aacagtgttc ttgatattat
    16141 tatccagacg ggcattattg gcttattttt ttggatgctt ttgtgtatat tttgggtttg
    16201 gcagtgttgg cggttatatc actcgtccgt tggcaaaaaa ttttccttag cctcgatttt
    16261 tgccttgctt gcttatagtt tgactttgat ggtgggaatt tccgacatta gcagtatttt
    16321 ttatttttgg gtgattgccg ctttggtgtc cgccggaaac cttagtctgg gaaagacggg
    16381 ctcgtcttgt gttttatttg cccgcacgaa gaagaatttt cgttttttta caagacgtcc
    16441 gtttaactta agtttgggag aatggttgat aaaaatcggt ggcggtgttt ttggcctttt
    16501 ggctctaggc cagatttatt tcagtctgtc tgccttacag gccgattatt actttttgca
    16561 gattcgtcga cttttaccgg ctcgagagta ttttaccgtt tctgtaattt tatcttatct
    16621 tgatagaagt gctctcaatc cagtggcaaa atcgtactat catacttctt tggcttcgta
    16681 tattttgtct gattggtcca atttggaaga tttaaattca cagtatttac tccgccatga
    16741 attatctcgt attttatctc agtctttttt gcctggatat aatgagcgtt tgttgtcggc
    16801 ccgtctgcaa tgtttcttat cggggccgga aacagcttct cccttatttg cagaattaat
    16861 cgctgccagt ccgcagcgtc cggcgtctta tcgagattat gcccagtgtt ggcaaataaa
    16921 tggtggaaat tcggaggcgt tatctttata tcgcgatgtt ctacgtttac tgccgtcagc
    16981 tgataattct ttactaaatg atgaccatcg tgcttattta aatttttata tttcccgtat
    17041 ttatacggag atggctttgg cccaggaaaa ctccggccag ttgactcaag cccttgaatc
    17101 cttacgtcta gcttattctg cttatcctga agaccttatt aatttacaaa aaagagctga
    17161 tatttatggg cgccaaaaag aatataatct tgctcttttt tggttggaac acgcctatcg
    17221 gcgccagccc ggttcttatt attggccgct ggcaatttca aatgtttata atcttctcgg
    17281 tgaagaggcc ttagcggcta gttggtatag tcaggcactt attcttaacc ccgatttaaa
    17341 gccggcacct tagccagatt ttattcatat gcttttaata ttgataatta ttattgccct
    17401 gggattgtcg tttggcagtt ttgccaattg cttagtgtgg cgactctatc gtcaagaaaa
    17461 gattagtggc cgttcctatt gtccgcgctg tttaaaaaag ctttggtggc acgataacat
    17521 tcctttgttt agctacttgc gcctgggtgg acgctgccgt ttttgtcatc aacctatttc
    17581 ctggcagtat ccggcggttg aagtgattgt agcgggattg tttgtatttt tttggtttaa
    17641 acacctcggt tttgcctggg tagatttaga gactttgagt ttgtttttta attttgattt
    17701 ttggctagct ttaattcgtg attatctagc ggtttttgtg ttgacggtgg tattcgtttt
    17761 tgacctgcgt ttttatttag tgtcaaatgc agtggtaatt ccggcggctc ttgtttttgc
    17821 tggtttaaat ttattattgg gagcttcgtg gtatgtttta tttctcggca ttgctggcgc
    17881 ggtggctttc tttggtttgc aatttttagt aacgcgcggc cgtggtttgg gtgaaggcga
    17941 tatttggcta ggcttgcttt tggcctctct tttccctgat tggagtctac ttattctggc
    18001 tattctttct gcctatcttt taggcactat tgtcggtctg gttttaataa ttgccggacg
    18061 gaaggaatgg agctctaaat tacctttagg cgttttttta gcagtagggg cgttgattac
    18121 tttagcctgg ggaccggcgc tgctgaccgc ttattggtct ttccagttgt gaagatttaa
    18181 aaaattgatt ataatgtaat aagtatggct taaatcgctg gatgtttatt ttttagctaa
    18241 aatccggcgt ctatttttca atatttatga aaaatgacat tattgctggt ttggatatcg
    18301 gctccaccgc tgtccgttta gtaattggac agaagatttc cggtcccacc ggtgatgaaa
    18361 ttcaaattat tggtgccgtt tcttctccta ccgccggcgt cagcaagggc atagtaacta
    18421 gcattgaaga agcaacttct tctatctccg cttgtctgga aaaagcggag cgtattgttg
    18481 gtgtgcctat taccagtgtc tgggtaggga ttaatgaccc taatataaaa tgtgagcgca
    18541 gcaaaggagt ggtggccgtt agtaagagcg atggtgaaat cagcgaggca gatgttaatc
    18601 gagcaattga agcggctaga gctttggccg taccggctaa ttatgaaatt ttacatgtca
    18661 tgccgattaa gtttagcgtt gataatcaaa tcgatatcaa agatcctatc ggtatgagcg
    18721 gtattcgttt ggaggttgaa gccttaatta tccaaggttt aagtactcag actaaaaatt
    18781 taactaaagc catttttcgc accggtttag aaattgaaga tttagttatt tctcctttag
    18841 cggcggcgga agccgtattg acccctaaac aaaaagattt gggcgtagcc ttaatcaaca
    18901 tcggttcttc caccacttca ttggctgttt atgaagagcg taatcttttg catgctgcgg
    18961 ttttgcctat cggttctgaa tatattacca atgatattgc cctcggtctg cgttgcccga
    19021 ttaatttagc ggaaaggatt aaattagaat atggcagcgc caatcctgat gcctttgaaa
    19081 gtgatgatga ggtggatgtt actcgtttgg ttaaagagga agaggttaat gatgatatta
    19141 gtttggtttc cagaaaatat attgctgaaa ttattgaggc tagagtagaa gaaatttttg
    19201 ataaggttga taaagaacta aaaaaaattg atcgttcagg aatgttgccg gccggcgtag
    19261 tctttaccgg ttccggcgct caacttgacg gcatgattga agcggctaag cgtaaattgc
    19321 gcttgccggc ggctttaggc cagagcaaaa atatcagcgt cgttattgat aaggtgaaga
    19381 gccctgaatt tttgccagct ttaggcttag ttctttgggg agcacatgaa tatccaagtc
    19441 aaggctcagg taatttgcga aagaatattg gcgatatttt tggtaaggca aaaaatattt
    19501 ttcaaaaaat tattccaaga taaactaaac gaagaatagc cgctttccct attcaaagta
    19561 agcggagatt cgatcagaat tatggcagag gtaaaaccag aaatagaaac atttgcaaaa
    19621 ataaaagttg tcggtgtcgg cggcggtggc ggtagcgcta tcaaccggat gattggaaat
    19681 ggcattagag gggtggagtt tgtggctatt aataccgata tccaagcttt gcattataat
    19741 aaggctagcg agaaaattca cattggtaag gcggttacta aaggtttagg tgcgggcatg
    19801 aatcctgatt taggcaagag cgctgctgag gaatcgcaga atgaaattag agaggcgctt
    19861 aagggttgtg atatggtgtt tgttacttgc ggcttaggtg ggggtaccgg ttccggcgct
    19921 tcgccgattg tagcccaaat tgctcgcgat ttaggcgctc ttaccgtagc ggtggtaacc
    19981 aagccttttc tttttgaagg cggccaaaga aagaatattg ctgatcgcgc ttttgccgat
    20041 ttagcggata aagtcgatac tatcattacc atttctaatg ataagctttt gcaggtggta
    20101 gacaaaagaa cttccttact tgaagctttt gccatcgctg atgatgtttt acgtcaagga
    20161 gttcaaggta tagcagaaat tattaccgta ccgggcgtca ttaatgctga ttttgctgat
    20221 gttaaggcgg ttatggctaa tgccggttcg gccttgatgg gtattggcca ggccagcggt
    20281 gaaaataagg caattgaagc ggctaaagcg gcaattaact ccaatttatt ggatatgtct
    20341 atcgatggtg ctcgcggtat tgtctttacc attaccggcg gtcctgattt gagcatgaat
    20401 gaagttagtg atgccgctaa ggttattact tccgccgccg atgaagacgc caaaattatt
    20461 tttggtgcag ttatcgacga caagatgaaa gaccagataa agattacggt cgtggctacc
    20521 ggttttgacg gtaagagcaa gagttcaaaa gtcgagctta aaagctatac tcccaatcct
    20581 tttgttgaga gtgagaatga aaaagaatca acacctgctt ccgctctctg ggggaacaag
    20641 aaggcaaaaa taccaactat caagaatata cctttacagc ctttagtgga gaagcgtcaa
    20701 gaaaaaatgt cggaaccaga aaagaccgaa gaagacgatt taagtgtgcc cgcttttatt
    20761 cggaagaaga tgagtaatta atgttttagg ccatgaatat tgatttttgc gtgcccgttt
    20821 acaatgaaga agaaattttc tcggctaacg cagaacggat ttggcgtttt ttacaatcat
    20881 tcatgtgtga atacgattgg cacttgattt ttatcgtcaa tggctccaat caaaaattta
    20941 ttgatttagt tcaagatttt gttaataaac acgggcctca atcggcctgt tttattattt
    21001 cagaaagtgg caagggaaga gctattaaat tttattttga tcaaagtaag gccgattttt
    21061 tggtatatat ggatattgat ttggccgtag atttgtttaa tttgcctcaa ttgctggcgc
    21121 cgcttttaag cgataaggcc gatctttgtt tcggctcgag aatgttgccg gcttcagtga
    21181 aaaatcgttc ttggtttcga gaaatcagct ccaaggtcta tcttaggctg tcgcgcaaat
    21241 tgttgggtca ccgttttact gatttacaat gtggttttaa ggctgtcagt caagaagcct
    21301 ggaaatcgat tactccctta attttagata aagcctggtt ttttgatact gagcttatct
    21361 gtttggcgca aaaaaaactt ttacgactcc aggaaatacc cgttgattgg tcagagaatc
    21421 gctatattaa gcgccgtagc aaagttaagg tttggaaaga ggcttgggtg ttcttatttt
    21481 tgacctttaa attaagaagt cgcctgcaga ggatgtgatc ttttgttttt tcttatattt
    21541 tctgtttaaa ggctgaataa ttatccacaa gccgcctggc aaaggggcgg tttttttaat
    21601 ttttgtatga atattggctg attttgacca gagtttttaa ttctgctata ataaaaataa
    21661 ttacaatatt tagtggttga tgatgaaaaa gccccacaaa atatagcttt tatcgttttt
    21721 tatgaaatgc cccgtctgct attataatga tactaaggtc gtggattctc gggtggcccc
    21781 cgatggtctt tccatccgtc gtcgccgcga gtgtctgaaa tgtaattttc gcttttctac
    21841 ctatgaagaa attgaaattt tagatttgat gattgttaag cgagatggcc ggcgtgaatc
    21901 ctatagccgg gaaaaattag tgaacggttt gagaaaatct ttagaaaaaa gaccggtaac
    21961 ggaagataat tttaaacgga ttatccatgg cattgaaaga gacttgcaga aattaagaca
    22021 aggcgaagtt acttcccgac aagtcggaca gatagtaatg aaacatctca aaaagatgga
    22081 tcaagtcgct tatatccgct acgcctcggt ttatgaatct tttaaggatg ccaacgaatt
    22141 taggcgcgag cttaataagc tgattaaaga taaaaaagaa aaataaatta aataatagaa
    22201 tggcgatggc gctttgaaga tttaaggtta gggtgattag ttttaaatct tcaatttcgc
    22261 gattgttttc aaccccatgt cagaaatcat cagccaaatc cggaaacgtt ccggcgacat
    22321 tgttggtttt agccaacaaa aaattaaaga cgctatctat aaagcggctt tatcagtggg
    22381 tatcgacgat gaagaaatgt ccgcccgttt tagtgaagag gtggtaaaaa agattgccgc
    22441 taaatttcac aagaatagca ttcctgcagt tgaagaaata caggacattg tggaagaggt
    22501 tttaattgaa catcgtcaaa tcaaaaccgt tagggcttat attatttatc gtgaccagca
    22561 tcgtcagtta agagagttga acaaagctac ctccgatgaa aaattgatgg aagattattt
    22621 gggtaaggcc gattggcgtt tgaaggagaa tagtaacatg agtttttccg ttcagggtct
    22681 taataattat atcgcttcct ctatttccgc ccgttattgg ttgaataagc tctatcccca
    22741 gacaatccgc gatgctcatg ttaatggcga tattcatatc catgatttag gcctgttggc
    22801 cccatattgc tgcggctggg atttgaaaga tttattagtt aaaggttttg gcggcgttcc
    22861 tgaaaaggtc caaagtaagc cagctaaaca ttttcgtagc gctcttggcc aaattgttaa
    22921 tttcttttat accatgcagg gagaggcggc tggagcgcag gcttttgcca attttgatac
    22981 ttatttagcc ccttttattc gttatgataa tttaagctat aaggaagtta agcagggctt
    23041 gcaggagttc atgtttaaca ttaacgttcc tactcgcgtc ggtttccaga ctccttttac
    23101 caacatcact ttggatgtaa ctccttctaa attagtgggc gaggagaacg ttattattgg
    23161 tgggcagata atgccggaaa aatataagga ctttcaagcg gaaatggata tgttcaacca
    23221 ggcttttgcg gaggtgatgt tggagggcga ttctactggg cgtgttttcg gctttccgat
    23281 tccgacctat accattgaca agaattttga ctgggagaga gaatgcttga aaccggtctg
    23341 ggagatgacg gctaaatatg gcattcctta tttttctaac tttgttaact ccgacatgga
    23401 cagggatgat gcccgttcaa tgtgttgccg cttacgtttg gataacaggg aattacgtaa
    23461 acgtggcggc ggtctcttcg gcgccaatcc tttaaccggc agtcttgggg ttgttacttt
    23521 aaatttagcg cgcatcggct atgtcgccaa gagcaaagag gaatttttgg aaagagttta
    23581 taaattaatg gatattgcta gagatagctt ggaaataaag cgcaagatta ttgagaaatt
    23641 ttccgatgac ggtttatatc cctattccac tcactatttg gcagacatta aggctcgttt
    23701 tggcgcttac tggcagaatc atttcaatac tataggtatt aacggcatga atgaggcttt
    23761 ggtaaattta ttaggtaaag atattactac ccccgaaggt catgccttcg ctttagaagt
    23821 tatggatgcc atgcgcgagc gtttgatgga ttatcagaac gaaaccaata atttatataa
    23881 tttagaggct accccgggtg agggctctac ttatcgtttt gctaaaaaag atttggaaat
    23941 ttatccagat atacattttg ccaatactga ggcagtgaaa aacggcgccg ctccttatta
    24001 cactaattcc actcatttac cggtaggttt ctctgatgat atttttgccg ctttagattt
    24061 gcaggatgat ttgcaggtta aatataccgg cggcactgtt cttcatggct atttaggcga
    24121 acgtctgcct gacggtaacg ccgctaaaaa attagtgcaa agaattatta ataattaccg
    24181 tttgccttat tttactatca ccccgacttt ttctatttgt cctaaacatg gatatttgtc
    24241 cggtgaacat aaatattgtc ctaagtgtga tgaagaaatg ggcttcacta gtgctgatat
    24301 gcaggccgag cgctgctccg attgttagtt tgttttattt ataaatttat tttataatat
    24361 acttaagtaa agcgttttaa ttaagctttt atacgcttta tcttaagtct caatttatat
    24421 gagccctatt accactcagc gccaagaatg catcgtttac agccgtgtcg tcggctggct
    24481 gaccccagtt aaaagttata atcctggcaa gacttccgaa tataaggatc gcaagacttt
    24541 caagattacc tgctctgagt aattaacttt tcagagggaa gcggggaaag ccgttattaa
    24601 aatcccggct atcctctggt tttcccatat gttaattggt ggtttagaaa aattaagcct
    24661 cttagattat cctaataatt tagccgctat cgtttttacc caaggctgta atttccgttg
    24721 tcatttttgc tataacccta tgcttgtctg gccccgctca gaaaagttgg cgccggacga
    24781 aaaagacaaa gataaaggtt atccccttat tcaggaggac gacctttttc tttttttaca
    24841 aaagagattg ggtaaaatag atggcgtcgt tattactgga ggcgaaccga ccttgcacgc
    24901 cgacttaccc tcatttatta agaagattaa ggccatggga tatttggtta aacttgatac
    24961 caacggcaca aattctttaa tgcttgagac tttattctcg gaaaaactag ttgattatat
    25021 agctatggac attaaagctc cttggccaaa gtatgaaaaa gttgtcggcg tcccggtaaa
    25081 tttagagaat ttgcaaaaaa gtgttaaaat aataatgtct agcggtctgc cctatgagtt
    25141 tcgcagcacc ttattacccg atttgcacga tgcaaatgat gtcgaagata tgggaaaaat
    25201 gattcagggg gcggaacgct ggtatttaca gaaatttaaa tcagacacta atctggttaa
    25261 tgttgatttt gaaggtaaaa atactttttt agataaagaa ttgaaagaat tagcgcttat
    25321 cggttccaaa tttgttaaag agtgccgagc gcgcgtttaa gtttatggac gacttaccaa
    25381 aggaagttga ttctctggcc tcaagcgagt ctgtaaaaac tgaggatttg gctctgctct
    25441 taaaagagaa tctggaaatg agtaaggaga taagggcgat ggtcaggcat attaataatt
    25501 atgtggcgtg gcagaggatt tttggctgga taaaagtttt gttaattgtc atacctttga
    25561 ttatcggcat tttatatctc cccccgtttc ttaaaggcct tttgagtcag tatgaatctt
    25621 taatcggcgg cggtttaggt agttagttta ttcttttgtt tattatatgg atcaagaaat
    25681 agcggttttt gcacaaacga ctttccgtaa ccaatttaag aagtttggaa ttaaaactga
    25741 cgatcgccgt cggcatatgt atttaattgg aaaaaccggt atgggcaagt ctaccattca
    25801 agagaatatg attgtcggtg acattcgtgc cggtcatggt ctggccgtgg ttgatcccca
    25861 cggcgattta gcggaaaaaa ttattgaata tatcccttca aaccgcattc aagacgtagt
    25921 ctattttaat ccttcagata ttgactaccc cattgctttt aatgtcttgg aacaagttga
    25981 tcctcatttg cgtcatttgg ttgcttccgg tcttttaggt gtgtttcaga aattatgggc
    26041 tgactcctgg ggacctaggt tggagtatat tttaagaaac actattttgg caattttaga
    26101 ttttcccggc tcgactcttt tgggggtggt gcgtatgctt tcggataaat cctatcgtaa
    26161 gcgcgtagtc gccaatataa aagatccggt ggttaaggcc ttctgggaaa aggagttcgc
    26221 ctcctatgcg gataagtttg cttcggaagc ggtctcgccg attcagaata aggttggtca
    26281 atttctttcc agttctctga tgcgcaatat cattggtcaa gtccgttcct ctttcaatat
    26341 tcgggaaatc atggaccaag gaaaaatttt aatcatgaat ttaagtaagg gaaggattgg
    26401 agaagataat tctgctcttt tgggggcaat gatgattacg aagattcaat tggcggcaat
    26461 gagccgggtt gatgtgcccg agaaagaacg taaagacttc tatctttata tcgatgaatt
    26521 tcagaatttt tccactgaca gttttgctaa tattttatcg gaggctagaa aatatcattt
    26581 aaacctcatt ttagctcatc agtatattga acagttgagc gaaaaggtca agccggccgt
    26641 ttttggtaac gtgggtacga taatagcttt tcgagtcggc gccgctgatg ctgaggaatt
    26701 agccaaggaa tttgctccag tttttaccga agaagatttg gttaatttgc ctaaatatga
    26761 aatgtatttg aaattgatga tagatggcgt tgcctcttct cctttttccg ctaaaggatt
    26821 gccgccgctt tccgatgaag aaaagactaa taatgtggat caagtgattg cttattctcg
    26881 gaagaaatat gctagcagtc gggctgaagt cgaagagaaa attttacgct ggcatgaggc
    26941 tcaagacgat attccctcca cccccattcg tccggtggcg ccggcttcgc ccctttttgc
    27001 catgtctcct aaatctttag aagcaaaaac cggagtgtct gccattaatc aacctacgat
    27061 tgccaggcaa tcagcagaaa aaaatattga aaggggaaaa gccattcctt cttcaagtcc
    27121 taaattgcaa gagcgtttga tctttcaggg cactaacacc gcggtcgccc cgataaaaca
    27181 ggcaggcgca tctcaatcta gcgctcccgt tttcgggata agagcagtaa aaaatccgtc
    27241 ggcagcgaaa ggttatcacg ctatttgttc gcgttgtggc gccgagacta ctactgtgtt
    27301 tgtgccggat ggtattagac cggttttttg taaagattgc ttgagtttga agcgtcaaga
    27361 aagaaaaaga gaggaggata ataggcgtca agctaaagca gaagagttac aagcttttaa
    27421 aaatattcct aaccctagcc cctctttgtc tttatcctct ttgcgccaag cccggccggt
    27481 agatttccgt ggccgtccgg aaaagatagt tcctccaatt aaaaaaaatg attttcaaga
    27541 agaaggacaa gacatggccg aagacgagct gattgatttt tctcggaggc cagaataatt
    27601 ttatgtattt agtgtttgat acagagacga ccggtttacc taaaaactat aaagcgccaa
    27661 ttagcgattt agataattgg ccacgtttag tccaattgtc atgggcttgg tttgatgccg
    27721 ccggcaaggc ttgggaccgt tatgattata ttattcgtcc cgatggtttt attattccag
    27781 aagaagcaac taagattcat cgtattagcc aggcgcaagc cgtcgctgaa ggtaaggatt
    27841 taaaagaagt tttattggag tttgccgaac acatagctcg cgcggaagcc ttggtgggcc
    27901 ataatattga ttttgacgat aaaattattg gttctgaatt ctatcgtaat cagattccta
    27961 atgaattggc agaagtaaaa aaaatatgta cgatgaaggc gggcgccgac atttgccgta
    28021 tcagcgatgg tcacggaggc tataaatggc ccaatttgtc ggaattacat cgtcatcttt
    28081 ttaaccgtga ttttactgag gctcataatg ccatggtcga tgttttagct tgcgcacgtt
    28141 gcttttttga actgcggcaa cgaggttttc ttatttaatt atgaattatt taaaaatagg
    28201 taaagctact gatttacaag ggaaggaccg tcgactctat cgtttttttg aaattttacc
    28261 gggtctgtta tctttttcga ctctattaat tcttctgata ttttcttatt ttcagccggt
    28321 atgggtggcg ttttttatta tcgcctttga cgtttactgg ttgctcctgg ttatttacct
    28381 agccctttat ttggtagcgg cttatcgtaa attaaaaaag aacacggcta ttaattggcg
    28441 gacgcggtgt gaagacttaa atcggggcga tttttccgat cctaaaattt ctccagacgc
    28501 tttagccaga cagggaaaac attggcaaga tttatggcat ttgttaatct tccctaatta
    28561 taatgagagt gaagatatta ttgccagcgc cttagaatct gttttacaag atgattttcc
    28621 ggcggaaaaa tttataatag ttttggcgat ggaggaaaga gcgggcgctt ccgcccacga
    28681 gcgggctatt gctattgaga aaaaatttaa gcatcgtttt ggtcgctttt ttgtgacttt
    28741 tcatcctgat ggtattgagg gtgaacttaa aggcaagggc gctaatcaag cttgggcgat
    28801 taagcgggtt aaagaggaat tgcttgacgt tgaaaatttt gattatgaca atattttagt
    28861 cagtgttttt gatattgata caatggttcg ccccggttat ttttttgctt tgagttataa
    28921 gtttttaacc gtttcctctc cttatcgtgc cagttatcag ccaatacccg tctatcataa
    28981 taatttatgg gaagcgccat ttttctctcg ggtagcggct tcttccaata ctttttggca
    29041 gatgatgatg caaatacgtg aagagcaatt ggttacatat tcttcccatt ccatgacgtt
    29101 aagagcttta gttgatattg gtttttggtc aaccaatatg gtgagcgagg attcccgtat
    29161 tttttggcat tgtttgcttt attacggtgg cgattatcga gttgaacctc tatattttcc
    29221 tgtttcaatg gacataactt ttgatcgtaa tttttggcag acagcgcgca gcttgtataa
    29281 acagcagcgc cgctgggcct ggggggcgga aaatgtgcct tatttaatat ttaacacaat
    29341 aaagagttgg catcgtatag accgccgtaa gcttatcagt catatattta tccaacttta
    29401 cggttttcat tcttgggcaa ctaacgcttt gatcattgcc gttatcggtt ggatgccgat
    29461 gcttttgggt ggggcgagat tcaatagtac tgttctttcc ggtaatctgc ccgccatcac
    29521 gcaatcatta atgaacatcg ccatgattgg tttggtttta tcggcgataa tttctacctt
    29581 gcttctaccg aaacgcccac ccaaatatac ggtttttaaa aatgtccgca tggttttaga
    29641 gtggatattc gtgcccttta ccattatttt tttcggcgct attccttgtt tggatgctca
    29701 gtggcgctta ttgcgaggaa aatatatggg tttttgggtg acaccgaagt cgcgtaatta
    29761 gattttttag ctgtcgtttt tctcatcttt ttgctatgat gataaaagca aaatatgact
    29821 aagcagaaaa tgatttccaa tttaaaggcg tctcgtcgtc gcgagacgaa ctttctttat
    29881 agatttttgg ttaatccgag aactttcgct attattgcct tggttttttt gttactaatt
    29941 tttttacctt tggctaaaaa ttatagccgc aagcgtttgg ttgagcaaga aattgctggt
    30001 attcagaaag aaattgatga ttttgaagca aaaaataggg atttgaaaga gatgattgat
    30061 tatttgcagt ctgaccagtc tttagaagaa caggcaagat tgaatatggg tttaaagcgc
    30121 cccggagaaa tagtggcggt gattcaggag gatacagcgg ttaatgccat ctcatcgccg
    30181 gttttagttg atgattctcc taattggcaa aaatggtggc gttattttgc taattaattt
    30241 atttaaaaac caaaagccag ctaattgctg actttctttg ttgacagtgt gagccgagaa
    30301 tgggacttga acccataacc tacggtttac gataccgttg ctctaccaat tgagctatct
    30361 cggctagtgt gagcgggtga gcggaatcga accgccgtct taagcttggg aagctcacat
    30421 aataaccatt atacgacacc cgccgcttca atattataag cagaagccgg aatcttagtc
    30481 aattcgaaac gttttaacat caatatttat gaaaaaaaga tatttcctca gtatttttat
    30541 atcactaagc ttagcggcgg gattcctatt gtttaaatct tctccagttt cagcctatga
    30601 agcctatgtt ggcatcactc cgcctgccga tactattgat tctgtcgatg agtttaataa
    30661 aaaaacgcta caagaaaatt tagtggctgg tcaagaacgg ttattacgtt tagagtcttt
    30721 tagcgtcaag gtgcccgccg aagcattaag taaaaatgca gttttgcggc tggataattt
    30781 gggcgaagat tttcctctac cttggaatta tgaacttttg ggccatgtct atcaatttga
    30841 ttttgcttct accagccaac attataatca aaaagttttt ttagatttaa gtatcgacta
    30901 cggagagaag aataattact ataaacagat attctttttt gatggctctt cacaccgatg
    30961 gcgtccattg ccttcaagcg acgatcctag caaccacaga attagcgcta aaataaattt
    31021 tccctttgct agagtggcgg ttttatataa cgatcaggtt ttaactgtcg gtcaagctag
    31081 ttggtatcgc ttccaaggag gtctttatgc cgcttctcct gatttcaaaa aaggcactgt
    31141 cttaaaagtc acgaatttag ataacaacaa gtctgtctct gtaaccgtga acgactatgg
    31201 gccagatcgc tcactatttt ctaatcgggt aatcgattta gatgctcgcg cttttgaaaa
    31261 aattgcttct ttagagacag gtattatcaa tgtaaagatt gaaacggaaa aaattatcgc
    31321 ccccgaagac agccaaaaac tccaagcggc ctcttcggat ttgaatttaa gctcgcaatc
    31381 ggctgtagtt ttaagggaaa gtgatggcaa gattttatat gacaagaatg cgactagtgt
    31441 ttcgcctctt gccagcctga gtaaattagt ggccgcgcaa gtatttttag acactaaccc
    31501 tgatttgaat aaagtggttt cttattccgt ccaagatgaa aattataatt atcagtattg
    31561 taaaccctgg gaatcggctc gtctgaccgt taaagagggc gatactttga ccattggtga
    31621 tttactttat tcggctttag tcggttccgc taataacgcc gttgaaagtt tggtgagagt
    31681 tagtggttta agtcgctccg aattcattaa aaagatgaac gataaagtta aatcttgggg
    31741 cgcgaataat actcattttg tcgaaccgac aggattgtct cccgacaatg tgagttctcc
    31801 tcttgattat gctataataa caagagagct ttttaagaat tctctattac aaaaaatcag
    31861 caccactgct cgttattctt tctccaccat taataccaaa gtcaggcata atcttaataa
    31921 taccaataat cttttactta ataataatta taaaattatc ggttctaaga ctgggtattt
    31981 ggacgaggcc ggttattgcc tgatgactag agtagattct gctgccggtc gcttaatagt
    32041 tgtcaatttt ggcgctaaga gcaaatctga taattttatg gataatgaat tgctaataaa
    32101 ctacggtctg aaaaagctat ctcaataaaa ttctatgata agcttactcc atgtttctaa
    32161 aagttattct cgtcgtaata agattttaag cgatgttaat ctccatatta aggccggaga
    32221 gtttgtttct gttgtcggtc aatccggtgc cggcaagacg accttagtca agcttcttat
    32281 cggtgaggag cgagccgacg aaggccaggt ggttatcggt gattgggata tcactcgtct
    32341 taaccggcat gaaattcctt atttgcgccg tcaaatagga gttatttttc aagattttaa
    32401 gcttttgccg aaaaagactt tacgcgagaa cgtcgctttt gctttagagg tttgcggttc
    32461 aagccaagcg aaaataaaga agattgtgcc tagtgttttg aaaattgtcg gcctggaaga
    32521 caaaggtgac cgttatccgc atgaagtttc cggaggtgaa cagcaaagag cggcaattgc
    32581 ccgcgcttta gttcatcagc cgcggatact tttagcggat gaacccaccg gtaatctgga
    32641 cgctattaat gctgaggaga ttatggaatt gctgttaaaa attaataaat ttggaacaac
    32701 ggtaattttg gtcacacaca ataaagagat agtcaatcgc ttgaatcgcc gagtgattac
    32761 tatggataag gggcgtgtgg tcagcgatca ggctcatggc aaatatttgc tctaaattat
    32821 cattatttat gctttctttt ttgcgcatca tcaaatttag ctttcaagat attgttcgta
    32881 atatttggtt gtcaatcgtt acaatcacaa ttttattatt ggctcttttt tccattaaca
    32941 cccttttgac ggtccgagtg gtcagtgata atgcggttaa tgccgtgaag gaaaaaattg
    33001 atattagtct ttatctgaag ccggaggcaa ccgagagcga tattgccgct ttgcgcagtc
    33061 gtttaagtag catgccggag attaaggctg ttaattatac ttctaagcag gcagctctga
    33121 attctttccg caataaatat cgtgataatc cagaaattct gcaagctttg cgcgaattag
    33181 gaaataatcc tttatcgccc agtttagcca ttactcccgc taactttgat gatacggaaa
    33241 atctggttaa taagctcagg cagcttgatt ccgatattat tgaatctcgc gatttcagta
    33301 ataatacggc ggtggtggct aaaattcaag ctataaccaa ccgaattaat gaaattggct
    33361 tagtcattat cgccattttt gttcttacca gtcttttggt ggtttataac tctatccgtg
    33421 tggcggttta tacgcataga aaggaaattg aaattatgcg tctggttgga gcttctaatt
    33481 tctttattta catgccttat ttcttctccg cttttttcta tgctttaatg agcgttttta
    33541 tcatgattat aattttctat cctttcctaa ctttgtttca accctatttg gaggttttct
    33601 tggccggtta taatgttaat ttgctcagtt attttgtgta taatttttgg ccgatattcg
    33661 gagcgcaatt cggtgttatt tttattatta ccctgattgc cagttttttt gcggtccgca
    33721 aatacgctcg tgtttaaatc aatttctaaa gcgcttgttc ggtgagaaag gcgctttttt
    33781 gttctttatt gactagattg atataattta ttaatattat ccaatattcg gggatcgtct
    33841 aat
//
LOCUS       DAOQSA010000006        31438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_827_length_31438_cov_9.017748, whole genome shotgun sequence.
ACCESSION   DAOQSA010000006 DAOQSA010000000
VERSION     DAOQSA010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 31438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31438
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_827_length_31438_cov_9.017748"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..84)
                     /locus_tag="PKK65_01185"
     tRNA            complement(2..84)
                     /locus_tag="PKK65_01185"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48..50),aa:Tyr,seq:gta)
     gene            complement(212..580)
                     /locus_tag="PKK65_01190"
     CDS             complement(212..580)
                     /locus_tag="PKK65_01190"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HNU81331.1"
                     /translation="MKKIFAIFVLSFLFVTNIALAQSGKSDNKTPTSVSITNPLAAGG
                     IDSFQTLIGRAINSILGVVGSLALVMFVYGGLLWMTSGGSNEQVKKGRDILIWATVGL
                     VIIFSAYGLVRFVIQGVGAA"
     gene            complement(678..1349)
                     /locus_tag="PKK65_01195"
     CDS             complement(678..1349)
                     /locus_tag="PKK65_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81332.1"
                     /translation="MARIIVGGNNVAPGSQSPNQIIQTGDSGGGKKLGKFLSYYYKIP
                     ITIVIAFVFYLVIRTIIPSENIIWWAVEIWAFVISLLVLWGLSETAKTKSKIGLSIFI
                     FILVIFILFIIKGYNEHHVQEMSTRNVSAISIPVSIPVLGPGTHIFFLKNIGDETGFF
                     QFPEGDIYYYQISSDDFGYKIFFMDDPTNPFPGNPSLVLPDKEHAIINIVATKANQYV
                     RVTVK"
     gene            complement(1355..2374)
                     /locus_tag="PKK65_01200"
     CDS             complement(1355..2374)
                     /locus_tag="PKK65_01200"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNU81333.1"
                     /translation="MKKLIAVLVLAIIIVVAIIFEPKIILVMAIFLVLVMKLFSVPEN
                     WEYQISFLGKYWKTCGPGLGWRVRWLSKVDHEVFMGDQTMRLALGDPSGLGGGVVDFQ
                     DGSAPITAFFFFKIFDSYKAAYSTADILRMIEEQADGTIRSFLSLFNIEDANTLKNNF
                     NLGWIAAMTIPVPGGNPPPLEQTHLWMMLNAWGVRPLNFTVTDIAIPAAIIEQRQRKQ
                     RAATDIDVATLEIEIAKKQAEKVVIDAEANRKAKVLAATGEAEGMDKISKAMAQRIRE
                     LMDKNGMTEEAARAHVISLAKAEALKEAKNVIWSEGNSAAGQGAAFGSGFNAVNSANQ
                     STPNQ"
     gene            2648..3079
                     /locus_tag="PKK65_01205"
     CDS             2648..3079
                     /locus_tag="PKK65_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81334.1"
                     /translation="MPRKIRKQDADRIRKKHQKEEEQKAPLLIFISTDEESVRQNEFD
                     SAGPVHRSLMEKVGKLAWLSYNICNLNGRSQEFNRMIVNAKFLLIHGKEKLRTEKLKT
                     LNHQIKHLKKANPRLKIFSTMDLPEAIKISTVNEITKYLKP"
     gene            complement(3316..3391)
                     /locus_tag="PKK65_01210"
     tRNA            complement(3316..3391)
                     /locus_tag="PKK65_01210"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3356..3358),aa:Thr,seq:ggt)
     gene            complement(3402..4214)
                     /locus_tag="PKK65_01215"
     CDS             complement(3402..4214)
                     /locus_tag="PKK65_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81335.1"
                     /translation="MLTTIAWILASLSLIVIIFIIVRKFPSLAILNVENIPGEKETKF
                     KEKIIRQRVERDFNRLGNSLDRWCRRLGGMISGFLKRYYQHLTKKRNDLRRQKKLTFI
                     EKREKISVLFNKAKVAMSLEKYEEAEKHLIEIISLDAKRLNAFLELAESYRLRKSFRE
                     AKETLEHALKLAQQLSRDPEMLEGVVLAEIRFSLAWICFQLGLQTEALEYGRQALDAE
                     PNNPRYLDLILSLSIMKKDKKSALDAWQRLASVNPENNKLPELHAKIEELPD"
     gene            complement(4230..4958)
                     /gene="tpiA"
                     /locus_tag="PKK65_01220"
     CDS             complement(4230..4958)
                     /gene="tpiA"
                     /locus_tag="PKK65_01220"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNU81336.1"
                     /translation="MKIFIANWKMQLSPEMSVRTARQFVRAFKNYQGQAVLCPDFLSL
                     AAVSKILAKSPLVLGSQDLSAFERGAYTGEISPVDLQSLGVKYSLIGHSERRSYLQES
                     DKLLAAKIKAAVSNKIIPVLCVGENALERKQDRAMAVIKAQLKGALQNLPTASWRSLI
                     IAYEPVWAIGTGLNCDREKALRVKMTINEWCRRGGFRKIPVLYGGSVKPENAASFLDR
                     NGFDGLLVGGASLDAQSFYKIAAL"
     gene            complement(4993..5922)
                     /locus_tag="PKK65_01225"
     CDS             complement(4993..5922)
                     /locus_tag="PKK65_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002620078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNU81337.1"
                     /translation="MSKILVTGGAGFIGSTLVDKLIDLGHEVTVLDNLVSGSRDYLNP
                     RADFWGIDILDPNLGNRFQEAKFDFVYHLAAQIDVRRSVADPEFDNQVNILGGLNVIK
                     AAQASGVKKIVFASTGGAIYGEAEEIPTSEYAPTYPVSPYGINKLALEKYLNYYYQVF
                     GLNYTILRFANVYGPRQYKGGEAGVIAIFTEAIAHDKTCVLYGDGKQTRDFVYVDDVV
                     EALVLAGEIDCRGEINISTAKEIDMWEMIAAIESAAGTKLKLELAPARPGEQRRSVLL
                     NQRALETLNWRPQMEFAEGVRRTYEWVKSNQLK"
     gene            complement(5867..6199)
                     /locus_tag="PKK65_01230"
     CDS             complement(5867..6199)
                     /locus_tag="PKK65_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81338.1"
                     /translation="MSKKEASSFGEVASNLMKGMKTKRLDSSQVASHKNRGYDCDVWD
                     DANRVGVRMRLCTSPPIVRISFPFGEGFFIGNNFHYLAILCFCAIIFLYVKDFSDWRR
                     WLYRFYFG"
     gene            6799..8838
                     /gene="gyrB"
                     /locus_tag="PKK65_01235"
     CDS             6799..8838
                     /gene="gyrB"
                     /locus_tag="PKK65_01235"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNU81339.1"
                     /translation="MAKNEQKNSNSNYGADAITVLEGLDPVRKRPGMYVGSTSATGLH
                     HLIWEVVDNCIDEAMAGYANEISVSLLPGRLVEVADNGRGIPVEKHKVTGLSALETVL
                     TKLHAGGKFGGGGYKVSGGLHGVGVSVVNALSEYLKAEVHRDGKSWVQEYKNGIPQGP
                     VKNIGKIKGTGTIITFKADASIFTELEFNWGKILDRLRQQSYLTKGITLNVLDKREGH
                     REKHYRFHFEGGIKSYIKSLHRGKTVKNETIFYVDKEINDCRVEVALQYNDEYNETVL
                     AFANNIHNPEGGTHLVGFRSALTRTLNNYGRTKNILKEKQENLSGEDVREGLTAIISV
                     KLPEPQFEGQTKSKLGNAEMKGYVEQIMGDAFNIFLEEHPKEAEAIIGKCVLSAQARF
                     AARTARATILRKGALEGMTLPGKLADCSSRHPEECELYIVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKILNVERARLDKMLANNEIKNLIIALGANIDDQFDLEKLRYHRIIIMTDAD
                     VDGAHIRTLLLTLFFRHFAPLIEQGHIYIAQPPLYQIKKGTNVAYAYSDEEKQAKIKE
                     WGGIAEEIETSEEEENGPQEIIEAEENEDENKDSAKSKTPAKIMIQRYKGLGEMNPEQ
                     LWNTTMDPAKRIIKQVTIEDAAKADQIFDMLMGDNVTPRKNFIQTHAKKVENLDI"
     gene            8840..9277
                     /locus_tag="PKK65_01240"
     CDS             8840..9277
                     /locus_tag="PKK65_01240"
                     /inference="COORDINATES: protein motif:HMM:NF014711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HNU81340.1"
                     /translation="MLPYIIYPIIAFCLAQGTKVLLKSTRKKHFNWRDIFAYSDMPSG
                     HTATVTALATIIGLREGLFSSGFALAFVFMTIIITDAIGLRNYLGQHGKILNVLVKDL
                     KEDDFLDNSYPKQLEHIGHTPGQVIIGGIIGIAVSLIGWSLGL"
     gene            complement(9543..10442)
                     /locus_tag="PKK65_01245"
     CDS             complement(9543..10442)
                     /locus_tag="PKK65_01245"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81341.1"
                     /translation="MDGDSLDQKDQSKSELVAPPLLLCLFLDGLGIAPATEVNAVTAA
                     KLPNFFKYIRDYPVTLLAGKTKDASRRYWSLGCGRADDDSHFLEADNCLSELISTAGK
                     RQLKIVASEQLLGLSLFFNNYREKPFGGEEIICFSTPAPEESLRPLSREIFRALEKAI
                     HAQAAPVIFASLPLAHEASARGDFKETVNSLQQIDKLLRKIINPVINVGGLVIITSAF
                     GNAERTRDLATDWADREPTANPVPFLLIGSQYQGKTIGLADPLDGDLSVLAPAGTLAD
                     FAPTVLHLLGIPKPDSMSGKSLI"
     gene            complement(10445..11758)
                     /gene="eno"
                     /locus_tag="PKK65_01250"
     CDS             complement(10445..11758)
                     /gene="eno"
                     /locus_tag="PKK65_01250"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNU81342.1"
                     /translation="MPKKNDNLIAALSAREILNACGYPTLEATLTLSSGQKVKASVPV
                     GSSPGVFGAKDLHDGDRRRYQGRGVLLAANHINELIAPRLLGISVDKQEEIDRILIDL
                     DATPDRRQLGANTIYAVSVVVARAGALSSGLELFTYISSHFKLAQPVCVPTPLFNMFN
                     GGSYADTNLDFQEFLLVPQKESPTFIASTCEGKAERMVRAGAEVFHALGKLLCESGYD
                     VDTGLEGGYAPDMDSSLKALELMLAAIVKAGYKPRIDFNLGLDIGSARLYDENAKQYI
                     FALDNSYFSAQSLLSLYNDWLQKYPLVYLEDGLAADDWPAWREVTASLGDKLLIAGDN
                     LFSSSLERLRRGIAEKAANAIVIKPNQIGTLTETVECAKLAQRQNYQLVISHRAGETN
                     DDFIADLAVALCAQYLKAGSLSRGERVAKYNRLTEIAAIMAASRK"
     gene            complement(11763..12935)
                     /locus_tag="PKK65_01255"
     CDS             complement(11763..12935)
                     /locus_tag="PKK65_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNU81343.1"
                     /translation="MKIRSLKKKKKWQGQTVLLRVDFNVPVSNGRIGEDYRITAALPT
                     IKYLREHGARLLIISHWGDPKTSDQALSTKPLARRLQKLLAAPVKFVPDLLGPKAQKA
                     VANLQEGEIVFLENLRSHPGEKKDDSSFAKELASLADVYVNEAFSVCHRAQASVSAIT
                     RYLPAFAGLQLEEELKNLQKILKPVKPLVVVMGGAKISTKAPLIKKLYARSAQIIIGG
                     GLANTFWKYQGLEIGKSLCDEESAGYLSQFLKDKKLLPKIILPVDVIVASAQGKIRRC
                     LPSEVKKSDCIFDIGPDSIALFSSYLKKAKTIVWNGPLGKFEDTPYKHGTMAIGTIIA
                     ACSTGRAFGLVGGGETVKALKMTKMESYVDWVSTAGGAMLAYLGGEKMPGLKNIIF"
     gene            complement(12940..14055)
                     /locus_tag="PKK65_01260"
     CDS             complement(12940..14055)
                     /locus_tag="PKK65_01260"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNU81344.1"
                     /translation="MKNNYLPLILIATFFGLLAGAAGALFANFYAANDSSFALNRELN
                     LSNYGYLSPSLVIRDAKKVVVNQDVKTDETIRSLKSSLLGVFVRAEDESSYYKLEQPF
                     ASALVATSDGWVMAAWPEELSKSVLERIPQDFVVIDSSRKIYKINQVVAKTDTIGNFV
                     FIHLLDASGFSVRRLVPDEEIKSGQSLLLAGGNNIFLLNYLSGKNFDDVILSSDDYAR
                     TFSLTYQIQAQPFFVFNLSGEIIGAIDNHGRWLTSPELSVYWQSLLKEKKLYWPSLGV
                     HYLNLSAVVGASDLPAKGAKLQGINNLPAVVKNSPAEKAGLMAGDIINRVNGIELNSE
                     NDLAVIISSYQPGDSLSIDYTRQGNSLQTEVILGKRE"
     gene            complement(14064..14831)
                     /locus_tag="PKK65_01265"
     CDS             complement(14064..14831)
                     /locus_tag="PKK65_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81345.1"
                     /translation="MLKKNLALLFNWYFWLTPILFYLGWRIAVIYPSSLWIVVLAACI
                     LLIPSVYEATERRLSWLSVAIYVNLAVLIVSFYLFISLIASPWILSLLWLIMLWHLYR
                     YLLFVKKFVAIKQNDYFIPIAFVSSLAASFFAAASLFGLQSFLTLSPWPLLVVFLVLL
                     IINTEALAIIQGWQNKHNQIIWPLIAFFTAEITMMITLLPLNYLISGILSALAYYSVI
                     NFVRLYLENNLKRHKIRNYAWFTAVSLAIILLSARWL"
     gene            complement(14841..17294)
                     /locus_tag="PKK65_01270"
     CDS             complement(14841..17294)
                     /locus_tag="PKK65_01270"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HNU81346.1"
                     /translation="MKKRYYFLIFIPFLALLAGGCSLPWSKKAPAENIEDNVIIIDNQ
                     AEISASGQMKKFSDYQELKSFLDTHAVETLRSYRGGLATPSIDSAVAANDAVFHESSS
                     QRVKNSSADSDYSQTNVQVEGVDEADIIKTDGRYIYAVVYQDLYIIQADPAEETRAVA
                     KISFTSRPSNIYLDGQHLVVIGADNQLMESSVYQNFRRQSPYTFVKIFDLSNPTSPKQ
                     IRDLDFEGSYRDSRVINGRLYLIIDNYNTYIPGERVVPRLVDGGKILTNDCSSADRCF
                     APDVYYFDLPYDSYNFISINTLDLRANDAPVAAQTYILNGAQTIYVSAKNIFITYTQH
                     LDESDLRLAVMKNVLGAKLSSAEQARVEKINQTDSDILSPSEKKQKLLQLFQNFLNAQ
                     SSAELVSLESEIAAALKQEYENQSQNWERTIIYKLALNGGKPVYRATGSVPGAVLNHF
                     SLDEDADGNLRLATTRSRNFSTLAGGDDSYSNVYVLSADLQLLGQVENLAPGERIYSV
                     RFLGKRAYLVTFKQTDPLFIIDLSDVKAPKFLGELKVPGFSQYLHPYDDNTLIGLGQD
                     TTVDAYGNVQIGGLKLSLFDVSNPTNPRELDSYVAGGAGSNSLALFNHKAFLFSRDKN
                     LLVIPASLTSSLDRGRVYFSGALVFAVEQGKFILKSQIDHSDGGKYQRSDYWCGAYCY
                     DNSVQRILYIKDALYTFSNKYLKVNRLSDFQSLQSVKLIDDTAADFEVESLPSENIIN
                     TVNNIEESPAVKPSPLGPSLPSAPIEPATIFVEESGDSNRLESATSSLPFEGDIITPE
                     ETASSTSETPENMSESLIP"
     gene            complement(17396..18145)
                     /locus_tag="PKK65_01275"
     CDS             complement(17396..18145)
                     /locus_tag="PKK65_01275"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNU81347.1"
                     /translation="MKILNFTFFFLFLLLAVTGVQANGLPDADRDGVPDKDEQEVYYT
                     DMYAVDTDGDGYSDFEEINRGFSPHNPEPVRLIDSDMDSDGLSDAWEIKFRTSPVLAD
                     TDGDGFKDGEEADYGFNPALAEGARLDVSLRVNLSSQTLDYLLNGYVWKSFPISSGKA
                     STPTPTGEFKVNNKIKKAWSQTYGLWMPYWLGLNAGSIGIHELPIWPNGYREGENHLG
                     TPVSHGCIRLGLDAASYIYDRIPVGTTVLIP"
     gene            complement(18160..19716)
                     /locus_tag="PKK65_01280"
     CDS             complement(18160..19716)
                     /locus_tag="PKK65_01280"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HNU81348.1"
                     /translation="MLLNLGFFLAATILSFILSLLVRRGALFLKITDCPDGVRKLHHK
                     ETALLGGVAIFLTFFIILFFASSQILSGDLEIHHWLGFFAGALILMIGGFLDDKYNLA
                     PHWQIIFPVLAALAPIVGGVDITKMSNPFGGFIIIPAVLSAVFIVLWLLGMMYTTKLL
                     DGVDGLVSGLGLIGSLVIFLFTSTTQYSQPDIAIAALIFSGACLGFLILNFNPAKIFL
                     GEGGSLLIGYVLGVLAVISGGKIAIALLIMGLPILDVFWTIIRRLLKGRNPFRFADRQ
                     HLHHRLLDLGLSSRQTVAVFYVFAAVFGLSGLFLQSRGKLFALLILLVIMLLLIIFFS
                     WWERARRPRLLFHICCAPCASYSTQTKLMPHYRVTWYFYNPNLSSLQEYERRLQAVRR
                     AADILKVDLITVPYRHEAWRELVRDRERDAERGARCQLCYRERLQAAYDFAKRRGFRY
                     FSTSLLVSPYKDGEAIRKICRELAAEGGPVFLNQDFQADNGFRASLDWARERGLYLQR
                     YCGCEFSFRK"
     gene            19797..20105
                     /locus_tag="PKK65_01285"
     CDS             19797..20105
                     /locus_tag="PKK65_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81349.1"
                     /translation="MKKKAEERISPFMKGNLSNDELEEVVRALSPQDLGEIKQLFLLK
                     IKEMESNQEALKKRLKRAECPEKIIKERLALTEANINEVRRCWHIFNNIFEERKSKKL
                     "
     gene            complement(20204..22867)
                     /locus_tag="PKK65_01290"
     CDS             complement(20204..22867)
                     /locus_tag="PKK65_01290"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNU81350.1"
                     /translation="MDNPSSLKIEIDKEYGQGAWFQGRFLYWRKNFSRSLIAVAAWRR
                     RADKILDIVVFIVALVGWLALVVWLFEQRTILTVSPLKILLFWENKHPLILIFLLSTW
                     FDLFLLYRQSQKKKAKKKINYRYWLSKVNNKPYYNIATTISAEMEAVLEKSFLIAAKL
                     KQPEVSVMHFFRALLTDITVQNVFIRLGINATSLVTKIDRHLSGPEGENRRPLISDNL
                     IAVMIDACADAISRHQDSLEPLNALAFCYQRNELLAEILYDLELDADKIDNVCEWFHI
                     NRQLVASYHSYRRLAGLKPGSGMDRAYTAVATPTLDHFSHDLTLAAKYGRLELCIGRK
                     VEINSIFEAMESGQAGILLVGASGTGKTMVINGVAQLMVEERVPKFLQDKRLVEIDVS
                     RLVSGATAADAQERLLTCINETARAGNIVLHIANIENLVGISSGGEGSLDLSEVLAEA
                     LVRKMIFCLATINSENYSRILEKTALGEAMTTVGIKEPSVNQAIQMLESKVAWFENKY
                     KIFITYNAIEQAVVMSSRYLHDKYLPDKAITLLQNAAMIAAKAKKRDPRQAICSKEQV
                     AAAISEMTGIPTQKLTESEGQRLLALEERIHQRMIGQDEAVSAVASSLRRARAQLKDA
                     KRPIASFLFLGPTGVGKTELAKTVSEVYFGDENYMIRLDMSEYQAADSIRKMIGDVDG
                     TLGYLTEAVRKKPFSLILLDEIEKAHPDILNLFLQLLDDGRLTDGQGRTISFTESIII
                     ATSNIGAVYIQEQIKAKTDIGLIKQELIDNQLNKFMRPELINRFDGIIVFKPLTEENI
                     FQITTLMLKGIKKRLAEKGINLKADRKGVMKLAQAGYDPKFGARPLRRLLQDRVEDSI
                     ANKILAGELKRRDTVVIDAQANIGVDKGRAL"
     gene            complement(22874..23245)
                     /locus_tag="PKK65_01295"
     CDS             complement(22874..23245)
                     /locus_tag="PKK65_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81351.1"
                     /translation="MAKSHFLTSVPQAVLSFLGNILFFPLWWYSLGFIMFASRIIAFW
                     RDEQKSLGFLVWLKNIFVPMYGQHDFAGRIISFFIRLIQIIFRGLILCFWIAIGLALM
                     VVWIALPLLLIAATAFQLLPA"
     gene            23579..26233
                     /locus_tag="PKK65_01300"
     CDS             23579..26233
                     /locus_tag="PKK65_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNU81352.1"
                     /translation="MFNKFTEKSQEAIINAQAIAANFGQPNIEALHILLSLLQQSESL
                     IKPILEKMKIDPEVLENQTINEIKKLPKSKVDLQNGPIQAVSGTNETALILERSKKEA
                     DGMGDEFISTEHILLALIGISSPAQTILFNFNVTYQTVVSILQELRGDERITDPSPEG
                     KFKVLEKYTSNLTELARQEKLDPVVGRDMEIRRVMQVLLRRTKNNPVLIGEAGTGKTA
                     IVEGLAQRIVAGDVPENLKNKEIVTLDLGALVAGAKFRGEFEDRLKAVLKEVQHQNGR
                     IILFIDELHTLIGAGSSEGSMDASNMLKPALARGELRTIGATTTKEYQKYIEKDAALE
                     RRFQPIMVNEPSVEDTISMLRGLKEKYEVHHGVRITDDALTAAAKLSNRYISDRFLPD
                     KAVDLIDEATSSLRMEIDSMPDELDQLKRRIKQLEINKAALLSSEKKSPKLRVINQEL
                     SKLKERSNQLELHWLNEKNIISQIRQHKKEIDELKIKAEISQRQGADLTEVAKIKYGL
                     IPELEAKIKASQDELIRIQKKGQRILKEEIGEEDIAKVVARWTGIPVNKMLADDLLKL
                     AKAEEELTQEVIGQEEAIAAVAKAIRRSRAGINEEKKPIGSFLFIGPTGVGKTELAKA
                     LARFLFNDENSLIRLDMSEYMEKHSVAKLIGSPPGYIGYDEGGQLTEKVRRHPYSVLL
                     FDEIEKAHPDMFNMLLQILDDGRLTDSKGRVVNFKNTVIIMTSNLGNEVIKQYAIGFG
                     EENEQPKKDQARNREMRDKIDKILKEHFKLEFLNRIDEIVLFKSLRPEDLEKIVSLEM
                     NKVEKRLNAKNIKLQISAKVKKILAEKGYDASYGARPLKRVIQNMILDELAMQIIENK
                     IQEGDKIRIDVDSEAKIKIKVEAQNKSK"
     gene            26361..27839
                     /locus_tag="PKK65_01305"
     CDS             26361..27839
                     /locus_tag="PKK65_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81353.1"
                     /translation="MKKHLNILAAVICIFFIIPAAGAQDIDPGFNPNFIISDEEMMDY
                     DSLSLAQIQNFLQKKNSYLANYSTTSAHGTQKSAAEIIYDATNNNYDCSGITLSDSPT
                     EAEKQLKCRKITTINPKFLLVLLQKESSLIEDPNPNQARLDWATGYGCPDNWTCNPYY
                     KGFGKQINSASLQFLAYMKEPQNYNYKAGQTYTFSNPYGTISNKTITVIPANQATAAL
                     YNYTPHVFDGNYNIYKLYQRYFPSTPARYPDGSLLQVKGEAGVWLIQDGLKRPFLTKS
                     ALLSRYSLDKIIMVEASDLESYDKGNPISLPNYSIVKAPDNTLYLIVGDEKRQFENQT
                     AFKSFGFNPEEIIAASTQDLSYYRNGAMLTANSVYPTGALLRDPQSGGVYYVQDGNKA
                     PIIDKVLLSTKFKGKTVQKASAEELARYTKISPVLFDDGELLKSESSSTVYLIANGKK
                     RPFLSGDDFERLGYKWSNIITVSPQLLYLYPLGEAITYKSAA"
     gene            27996..28802
                     /locus_tag="PKK65_01310"
     CDS             27996..28802
                     /locus_tag="PKK65_01310"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81354.1"
                     /translation="MPIVSASFLPPSPLLIPEIGKQNNQMLQKTSQAYRQMAAIFEKE
                     EIEIIIVISPYGLSQNENISLNIAPQLQISFQEFGYLATIKTLNPAWRLADAIVKEMG
                     HDYPVKLSSQEKLDYGSAVPLALLTENIIIKTLPLFPANQKNRAYHYEFGLKLGKILK
                     KRPEKIALVASGELSRCLKKNSPGGYSPKGARFDNRVIEYLNDSENGLNKILALEDKI
                     GEEAKEGGLKQLSLLIGAIGENCQAQILAYQNDFGIGYLSVNFNLQIATI"
     gene            28811..29119
                     /locus_tag="PKK65_01315"
     CDS             28811..29119
                     /locus_tag="PKK65_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81355.1"
                     /translation="MENIADLISQRQQQKAPAYPWQELALRIIKELSIPGFKRSAVFK
                     ICKELSPVLVERALNDTKELCRNGVRWKYFFKIADQYLAVKNKNNKKYKKEKNEKYQR
                     "
     gene            29100..29585
                     /gene="def"
                     /locus_tag="PKK65_01320"
     CDS             29100..29585
                     /gene="def"
                     /locus_tag="PKK65_01320"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009145214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNU81356.1"
                     /translation="MKNIKGKIMEIVYHPHPILRRQADPVKVEKIKSPEFKQFLADME
                     ATMLAKDGAGLAAPQVGISEKIVVIAGKNGNNLILINPQITHRSWGKIISEEGCLSII
                     DNRGRIIYGKVERHKHVTCQYIDAQGKKKKIQAEEPLARVIQHELDHLDGILFIDRLI
                     D"
     gene            complement(29630..30244)
                     /locus_tag="PKK65_01325"
     CDS             complement(29630..30244)
                     /locus_tag="PKK65_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNU81357.1"
                     /translation="MLITLLNYLLNFAQEIGYWGVVFLMTLESSFIPFPSEVVVPPAA
                     YLAAQGSLNIWLVILAGVAGSLIGAFINYFLAYYLGRPLIYRLAQTKVARFLLIRPAQ
                     IERAEMFFSRYDSGSTFFGRLVPVVRQLISLPAGFVKMPLWRFSFYTFLGSALWTLIL
                     AALGYFFGANEAAWKRYYLEISWALVAFGVVIALYFFWRYRERK"
     gene            30359..31297
                     /gene="fmt"
                     /locus_tag="PKK65_01330"
     CDS             30359..31297
                     /gene="fmt"
                     /locus_tag="PKK65_01330"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNU81358.1"
                     /translation="MTAKIRTIFMGTPKFAVPGLQALLESPDFNIVGVYTQPDKPVGR
                     KQILTPPPIKALAVKNNIPIFQPKKIKTETENIKNLKPDLIVVIAYGQIIPQAILDIP
                     QYGCINVHASLLPKYRGASCLAAPILNGDEKSGITIMKMDAGMDTGDIIKQISVKLSD
                     KETLNSLHDRLSILGAEILTDTLKKYINGEIKTQKQDESKASYVSLIKKENGRLQVDL
                     SATALERKVRAYNPWPGAFLRLNNGEKIKIIEAQAEKAVHQGKIGEIYIKNGHLALNC
                     GQDILFILKLQRENRKVMNASDFLKGNQDILNLTAI"
     gene            31301..31372
                     /locus_tag="PKK65_01335"
     tRNA            31301..31372
                     /locus_tag="PKK65_01335"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31334..31336,aa:Glu,seq:ctc)
BASE COUNT        10516 a         6996 c         5808 g         8118 t
ORIGIN      
        1 gtgggccggg ctggattcga accagcgaag gcgcgagcca acagatttac agtctgtccc
       61 gtttaaccgc ttcggtaccg acccttttaa aacaaagcgc ctgaattcag gctgtttcta
      121 ttatataaaa ttttcagtaa ttgtcaaaca aaaaccgcgc taatcgctga agcgccgcgc
      181 ggtttaaaaa tctgctaaaa aatgaaaagc ctcaggcggc cccgacaccc tggatcacaa
      241 agcgaaccaa gccgtaagcg gaaaagatga taaccagacc aacagtcgcc caaatcagaa
      301 tatctctacc ttttttcacc tgttcattgc tgccgccgga agtcatccac agcaaaccac
      361 cataaacgaa cattactagg gctaaagaac caaccacgcc gaggatacta ttaatggctc
      421 gaccgataag agtctggaaa gaatcaatgc cgccagcggc taaagggtta gttatgctaa
      481 cagaagtggg ggtcttatta tcacttttgc cggattgagc taaagctata ttcgttacaa
      541 atagaaaaga taagacaaaa atagcaaaaa tctttttcat aaaaataaaa aattatattt
      601 acttacctaa ttatattata acaattttta aaaaaataaa aaagggagcc gcgattgcag
      661 tccccttaaa taatttttta tttcacagta acgcgaacat attgatttgc cttagtggca
      721 acaatattga ttattgcgtg ctccttatcc ggtaatacca agctgggatt accgggaaaa
      781 ggatttgtgg gatcatccat aaaaaatatt ttatacccga aatcatcaga tgaaatctgg
      841 taataataaa tatctccttc tggaaattgg aaaaaaccag tttcatcacc aatgtttttc
      901 agaaagaaaa tatgcgtccc cggccctaag acaggaatag agacaggaat agaaattgct
      961 gatacgtttc gggtagacat ttcttgcacg tgatgttcat tatacccttt aattataaag
     1021 agaataaata ttactaaaat aaaaatgaat atcgagagtc caatttttga ttttgtttta
     1081 gcggtctcag aaagacccca caaaaccaaa agagaaataa cgaatgccca aatctctacc
     1141 gcccaccaaa ttatattctc cgaaggaata atagtcctga taacgagata aaagacaaac
     1201 gctataacta tagtaatcgg aattttgtag taataagaca aaaatttgcc taatttctta
     1261 ccaccaccac tatcgccagt ctgaatgatt tgattcgggc tctgtgaacc cggagcgaca
     1321 ttattacctc caacaatgat tcgtgccatg gcgattattg attaggagta ctctggttag
     1381 ccgaattaac agcattaaag ccgctgccaa aagctgcccc ctgaccggcc gcagaatttc
     1441 cttccgacca aataacattt ttagcctctt ttagggcttc cgcttttgcc aaagaaataa
     1501 cgtgtgctct ggcagcctct tcggtcatgc catttttatc cattagttct ctgattcgct
     1561 gagccatagc tttagaaatt ttatccatac cctcagcttc accggtagcc gctaagactt
     1621 tagctttacg gttagcctcg gcgtcaatca ctaccttttc cgcttgtttt ttagcgattt
     1681 caatctctaa ggtagccaca tcaatatcgg tagcggcccg ctgttttctc tgcctttgct
     1741 caataatggc agcgggtatg gcaatatcag tcactgtaaa gttcaaaggc cttacccccc
     1801 aagcatttaa catcatccaa agatgtgttt gctctaatgg cggaggatta ccgcctggca
     1861 ctggtattgt catagccgct atccacccta aattaaaatt attttttaag gtgttagcgt
     1921 cctctatatt aaatagcgac aaaaatgatc tgattgtccc atcagcctgc tcctcaatca
     1981 tccttaaaat atcagccgta gaatatgcgg ctttatatga atcaaaaatt ttaaagaaga
     2041 aaaaagcagt aataggggcg gaaccatcct ggaagtcgac gactccgccg ccgagtccag
     2101 aaggatcgcc cagcgccaat cgcatagtct gatcgcccat aaaaacctcg tggtcaacct
     2161 tgctgagcca gcgcacacgc caacccagac cgggcccgca ggttttccag tatttcccta
     2221 aaaaggaaat ttgatactcc caattttctg gtactgaaaa caatttcatc accaatacca
     2281 aaaatatcgc cataaccaaa ataatttttg gttcgaaaat aatagcgaca acaataatta
     2341 tcgccaaaac caataccgca attaattttt tcatttttcc tacgtttttt aattttaatt
     2401 tttaatgttc ggtctaatcg acaggcgtat ttaccctatc ttattagacc ctaaattata
     2461 ttattttttt aatattttgt caatagtaga taactagtct attgttacat tcttatacta
     2521 tttaacacga tataatagat taacgatata tactaatatt aaataaaata atcataaaaa
     2581 cttaacttga cataaggaaa taattattct ataataaggc aaaattcagt atttaaattt
     2641 ctttacaatg ccaagaaaaa ttagaaaaca agatgcagat cgcatccgta aaaaacacca
     2701 aaaagaagag gagcaaaaag cacccctgct catattcatc tcaaccgatg aagaaagcgt
     2761 cagacaaaac gaattcgata gcgctggtcc ggttcatcga agtctgatgg aaaaagtcgg
     2821 taaactcgcc tggctatcat acaatatctg taatcttaac ggccgtagtc aagaatttaa
     2881 ccggatgata gtcaatgcta aattcttgtt aatccatggc aaagaaaaat taagaacgga
     2941 aaagctcaaa acgctcaatc atcagattaa acatttaaaa aaagctaatc cgcgactgaa
     3001 aattttctca actatggact tgccggaagc gatcaaaata agcaccgtaa atgaaattac
     3061 aaaatacctt aagccttaag tcatgaaaaa attagcgccc atactccctt aacacaaccg
     3121 ttacccgtca catccccaaa attcatattt cgaccgcggc aggactgttc accgcaatat
     3181 tgagaaaatt cctgagaaaa aagtgagggc tgatgtgctt tttctgcctc ttctttgaaa
     3241 gcgaagtcca ctttctagaa aaagaaaaga actattaaaa aagactgccc cttcaaggta
     3301 gtcttctttc ttttctggag ccgttttcgg gacttgaacc cgagaccttc tccttaccat
     3361 ggagatgctc taccaactga gctaaaacgg cttttatctt cttaatcagg caattcctca
     3421 atcttagcgt gcaattccgg caacttatta ttctccggat tgacagacgc tagtctttgc
     3481 caagcgtcaa gcgctgattt tttatctttt ttcattatac ttaaactcaa aattaaatca
     3541 agataacgtg gattattcgg ttcggcgtca agcgcttgac gtccgtactc taaagcttca
     3601 gtttgcaaac ctaattgaaa acaaatccaa gctaaagaaa aacgaatctc tgctaacact
     3661 actccttcta acatttctgg atcccggctt aattgctggg ctaactttaa agcatgctct
     3721 agagtttcct tggcttcacg aaaactcttg cgcaaacggt agctttcagc taattccaaa
     3781 aaagcgttca aacgtttagc atcaaggcta ataatttcaa tcagatgctt ttccgcttct
     3841 tcgtattttt ctagagacat cgctaccttt gccttattga ataatactga aatcttttcc
     3901 cgcttctcaa taaaagttag cttcttctgc cggcgcaaat cattgcgctt cttagttaaa
     3961 tgttgataat aacgctttaa aaatccgctt atcattccac ccaagcgccg gcaccagcga
     4021 tcaaggctgt tgccgagacg attaaaatca cgctctaccc tctgacggat aatcttctcc
     4081 ttgaattttg tctctttctc cccgggaatg ttttccacgt ttaaaattgc cagcgaagga
     4141 aattttctga caataataaa aataataaca attaaagaaa gagaggctaa aatccaggca
     4201 atagtggtaa gcataatgat aataaaaaat taaagggcgg caatcttata aaaactttga
     4261 gcatcaagac tggcgcctcc gactaatagt ccgtcaaaac cgttgcgatc aagaaaagaa
     4321 gcggcatttt ccggcttgac gctgccgcca tataaaaccg gaattttcct aaagccgcca
     4381 cgccgacacc attcattaat ggtcatcttg acgcgtaagg ctttttctct atcacaatta
     4441 agaccagtac cgattgccca taccggttca taggcgataa ttaaagaacg ccaagacgct
     4501 gtcggtaaat tttgtaaagc tccttttagt tgcgctttaa taacagccat ggctctgtct
     4561 tgctttcttt ctaaggcgtt ctcgccgaca caaagaaccg gaataatttt attacttacc
     4621 gccgccttaa tcttagctgc caacagttta tcagattcct gcaaataaga gcggcgttcg
     4681 ctatgaccga ttaaagaata ttttacgcct aaagattgta aatcaacagg agaaatttcg
     4741 ccggtgtagg caccacgttc aaaggcagat aaatcttgag aaccgagaac cagaggactc
     4801 ttagctaaaa tttttgaaac cgctgctaag gaaagaaaat ccggacacag caccgcttga
     4861 ccttgataat tcttaaacgc gcgcacaaac tgccgggctg tccgcacaga catctctgga
     4921 ctaagttgca ttttccaatt ggcaataaat attttcataa aatttctaac cacgaccata
     4981 aaaaggtcgg ttttatttta actgattact ctttacccat tcataagtac ggcgcacacc
     5041 ttcggcaaat tccatctgtg gccgccaatt taaagtctcc agcgctcgct ggtttagtaa
     5101 gacactgcgg cgctgttccc ctggtctagc tggagccaat tccaatttta acttggtgcc
     5161 agcagcgctt tcaatcgccg cgatcatttc ccacatatca atttctttag ccgtactgat
     5221 attaatttcg ccgcggcaat cgatttcacc agccaaaact aaggcctcaa caacatcatc
     5281 aacataaaca aagtcacgag tctgtttgcc gtcgccataa agaacacatg ttttgtcatg
     5341 cgctatcgct tctgtaaaaa ttgcaatcac tcccgcctct ccgcctttat actggcgcgg
     5401 tccgtagaca ttagcaaaac gcaaaatcgt ataatttaaa ccaaaaacct gataatagta
     5461 attcaaatat ttttccaaag ccaatttatt gataccataa ggagaaaccg gataggtcgg
     5521 agcatattca ctggtgggga tttcttccgc ctcgccataa atagcgcctc cggttgaagc
     5581 aaaaacaatt ttttttaccc cactagcctg cgccgcttta ataacattca aacctcccaa
     5641 gatattaacc tgattgtcga attccgggtc agccaccgac ctccgcacat caatctgcgc
     5701 cgctagatga tagacaaaat caaacttagc ctcctgaaaa cgattaccga gattggggtc
     5761 taaaatatct attccccaaa aatcggctcg cggattcaaa taatcgcggg aaccagaaac
     5821 taagttatcc aaaacggtaa cttcgtgtcc taaatcaatt aatttatcaa ccaaagtaga
     5881 accgataaag ccagcgccgc cagtcactaa aatctttgac atataagaaa ataatagcgc
     5941 aaaagcaaag aattgccaaa tagtgaaagt tgttgccaat aaaaaaacct tccccgaaag
     6001 gaaagctaat gcggacgata ggcgggctgg tgcagaggcg cattcggacc ccaacacgat
     6061 tagcatcatc ccaaacgtcg caatcatagc ctcggttttt atgactggcc acttgacttg
     6121 aatccaacct ttttgtcttc attcccttca ttaaattact ggctacctcc ccaaaactgg
     6181 atgcctcttt ttttgacata aaacttcttt taaaatgaac gatagaaatc aatcttaaaa
     6241 aactaaataa aaaaatatac agaaaacaac cagctagacc atattatcca agaattagcc
     6301 gattacacca gctgtcactt ctctacggag gcgaaatatt ttcagctgtt cggctataaa
     6361 aagacggaag aacacattaa aattcatgaa caggataaga gaaaaatcaa agaatgaccc
     6421 cagtgctaca attaagataa aaacccggtg attttctttg aaatatccga atttctccaa
     6481 aattggtgga cctagcatat taataatacc aaccgcgctt atgttccctt tctccaagct
     6541 aatggcgtca aatagtatct tttaaagaac ttctaaacat tacggaagtt tttatattcc
     6601 caaaacagtc cgcacaaaag gattattttc ggtagcttta aaacaataaa attattaatt
     6661 aataatctct atttttagct aaaaataaag cttttgacaa gtttgcttta cttagataat
     6721 tttgctataa taaaattaag aaactgttag taaaacttat taaaatacga ttccgcaaac
     6781 aggaattttt ttctttctat ggctaaaaac gaacaaaaaa atagtaatag taattatgga
     6841 gccgacgcca tcactgtttt agagggcctc gacccagtca gaaaaagacc gggcatgtat
     6901 gtcggttcga cttccgctac tggtttacat catttgattt gggaagtagt ggataattgt
     6961 attgatgaag cgatggctgg ctatgctaac gaaatttccg tttcccttct gccgggacgc
     7021 ctagttgaag ttgctgacaa cggccggggt atacccgtag aaaaacataa ggtaaccggc
     7081 ctgtctgctc tggaaacagt tttaaccaaa cttcatgccg gaggcaaatt cggcggcggc
     7141 ggctataaag tctcaggcgg cctgcacggc gtcggtgttt ctgtagtaaa cgctttaagt
     7201 gaatacctaa aagcggaagt ccatcgtgat ggtaaatctt gggtgcaaga atataaaaac
     7261 ggcattccgc aaggaccagt aaaaaatata ggaaaaataa aaggcaccgg cactatcatc
     7321 accttcaaag cggatgcttc catcttcacc gaattagaat tcaattgggg aaagattctt
     7381 gaccgtttac gccagcaatc atatcttacc aaaggtatca ccttaaatgt tttagacaaa
     7441 cgcgaaggtc accgagagaa acattaccgc ttccattttg agggcggcat caaatcatac
     7501 atcaaatccc tacaccgcgg taaaaccgtc aaaaacgaaa cgatctttta cgtagacaaa
     7561 gagattaacg actgccgtgt cgaggttgcc ttgcaatata atgatgaata taatgaaacc
     7621 gttctcgctt ttgccaataa tatccataac cccgaaggcg gcacgcactt ggtcggtttc
     7681 cgcagtgccc ttactcgcac tctcaataat tatggacgca caaaaaatat cctgaaagaa
     7741 aaacaagaaa atttgagtgg cgaagatgtc cgcgaaggcc tcaccgccat tatcagcgtc
     7801 aaattgcctg agcctcaatt tgaaggacaa accaaaagca aactcggcaa tgccgaaatg
     7861 aaaggctacg ttgaacaaat aatgggcgac gcttttaata tctttctaga agaacatcca
     7921 aaagaggcgg aagcaattat cggcaagtgc gtcttgtctg ctcaagcacg ctttgccgcc
     7981 cgcactgcca gagccactat tctgcgcaaa ggcgctctag agggtatgac tctaccggga
     8041 aaactggccg actgttccag tcgccatccg gaagaatgcg aactttatat cgttgagggc
     8101 gactccgccg gcggtagcgc caaacaaggc cgagaccgta aattccaggc gattctgccc
     8161 ttacgcggta aaatccttaa cgttgaaaga gcgcgcttgg ataaaatgct ggccaataac
     8221 gaaattaaaa atttaattat tgctctaggt gccaatatcg acgaccaatt cgatttagaa
     8281 aaactaagat accaccgcat cattatcatg accgatgccg acgttgacgg cgctcatatc
     8341 cgcaccttgt tattaactct attcttccgc cacttcgccc ccttaattga acaggggcac
     8401 atctatatcg cccaaccgcc gctttatcaa attaaaaaag gaacaaatgt agcctatgct
     8461 tacagtgacg aagaaaagca agcaaaaata aaagaatggg ggggtattgc cgaggaaata
     8521 gaaaccagcg aagaagagga aaacggtcct caagaaatta tagaagcgga agaaaatgaa
     8581 gatgagaata aagacagcgc caagagtaaa acgccggcta aaataatgat tcagcgctac
     8641 aaaggtttgg gggaaatgaa tccggaacag ctttggaaca caaccatgga tccagctaaa
     8701 cgcatcatca aacaagtaac gattgaagat gctgctaagg ccgatcaaat attcgacatg
     8761 ctaatgggtg ataatgttac cccccgcaaa aactttattc aaacccatgc caagaaagta
     8821 gaaaatttag atatctaata tgctgccata cataatctat ccaattattg ccttctgtct
     8881 agctcaaggc acaaaagtat tgctaaaaag cacgcgcaaa aaacatttca attggcgtga
     8941 catctttgct tattccgata tgccgagcgg ccatactgct actgtcaccg ccctagcgac
     9001 tattatcggt ttacgtgaag gtcttttctc ttccgggttt gctctcgcct tcgtctttat
     9061 gacgattata atcaccgatg ctatcggctt acgcaattat cttggccagc acggcaaaat
     9121 ccttaatgtt ttagttaaag atttaaaaga agacgacttt ttagacaatt cctatccgaa
     9181 acaattagaa catatcggcc acactcccgg ccaagtgata atcggcggca taatcggtat
     9241 agccgtcagc ctcattggct ggagcttagg actgtagaaa ataataaaaa aaccggtctt
     9301 aaaaacacgg tttttttctt attaacaaag tcagtaacta ccatttacaa aatgaaattg
     9361 gcgaccagat atccataata aattttaaat cgtcgatcaa attattgaac ctggcctttc
     9421 ttgtttagcc aagcgtacgt ccgtcatttt tgaaatatca ttattcccta aacgctcgag
     9481 tgatttagcc tcttgctctt gttttggaat ttattgttta tattaaaatt aactatttat
     9541 ttttaaatta aactctttcc actcatactg tcaggcttag gaatgccgag caaatgtaaa
     9601 acggttggag caaagtcggc taatgttccg gcaggcgcca aaacgctcaa atcgccgtct
     9661 aaaggatcag ctaggcctat agtcttacct tggtactgag aaccaataag taaaaaagga
     9721 acaggattag cagtaggctc cctatctgcc caatcagtcg ccaaatcacg agtgcgttcg
     9781 gcgttaccaa aagccgaagt aataataacc aagccaccga cattaattac cggatttatt
     9841 atctttcgca gcaacttgtc aatttgctga agactgttaa ccgtctcttt aaagtcacct
     9901 ctggcggaag cttcatgggc taaaggcagg ctggcaaaaa taacaggcgc cgcctgagca
     9961 tgaattgctt tttctaaagc tctgaatatc tctcgactta aaggacgtaa actttcttcc
    10021 ggcgccggcg ttgaaaaaca aataatttct tcgccaccaa aaggtttctc tcgataattg
    10081 ttaaaaaata gacttaaacc caaaagctgt tcactggcaa ctatttttaa ttgtcttttc
    10141 cccgcagtcg aaatcaattc tgacaggcag ttatcggctt ctaaaaaatg agaatcatca
    10201 tcagcacggc cgcaaccgag ggaccaatag cgacgggagg catccttagt tttaccagca
    10261 agcaacgtca ccggataatc acgaatgtat ttaaaaaaat ttggcaactt ggcggcggta
    10321 acggcattaa cttcagtggc gggggcgatg ccaagaccat caaggaataa acataacaat
    10381 aagggcggcg cgaccagctc cgatttagac tgatcttttt gatcaagact gtctccatcc
    10441 ataattattt cctgctagcc gccataatag cggcaatttc ggtcaaacgg ttatacttag
    10501 cgactctttc cccacgtgac agagaaccgg ctttcaaata ttgagcacaa agagcaacgg
    10561 ctaaatccgc aataaaatca tcattggttt cgccagcacg atgggatatg accagttgat
    10621 aattctgacg ttgagctagt ttcgcacatt caacagtttc cgtcaaggtt ccaatttgat
    10681 ttggcttaat aacaatagca ttcgcggctt tttccgcaat accacggcgc aatctttcca
    10741 agctgctaga aaataaatta tcaccggcta tgagcaactt atctcccaaa gaagctgtca
    10801 cttctcgcca agctggccaa tcatctgccg ccagaccatc ttcaagataa actaaagggt
    10861 atttctgtag ccaatcatta tataggctca acaagctctg cgcgctaaaa taagaattat
    10921 ctagggcaaa aatatactgc ttagcatttt catcatacaa acgggccgaa cctatatcta
    10981 aaccgagatt aaaatctatc cgcggcttat agccggcttt cacgatagcc gccaacatca
    11041 actccaaagc cttgagactg gagtccatat ccggagcgta gccaccttct aagccagtat
    11101 caacatcata accggattca cacaaaagtt tgcctaaagc atgaaaaacc tctgctcctg
    11161 ccctaaccat tctttccgcc ttaccttcgc aagtactagc aataaaagtc ggcgactctt
    11221 tttggggaac aagtaaaaat tcctgaaagt ctaaattggt atcagcataa ctgccgccgt
    11281 tgaacatatt aaaaagagga gtgggtacac aaacaggctg agcaagttta aaatgactgg
    11341 aaatataggt aaataattcc aaaccggaac ttaaagcgcc tgcccgcgcc accactacag
    11401 acacggcata aatagtattt gcgcccaact gacgccgatc gggcgtagcg tccagatcaa
    11461 taagaatacg atcaatctct tcttgtttat ctacggatat acccaacaaa cgcggagcaa
    11521 tcaactcatt aatatgatta gccgccaata aaactcctct accttgatag cgccggcgat
    11581 cgccatcatg taaatcttta gcaccaaata ctccgggact cgaaccaaca ggaacgcttg
    11641 cctttacctt ctggccagaa gataaagtca acgtcgcttc tagggtcgga tagccgcacg
    11701 catttaatat ttccctagca cttaaggcgg caattaaatt atcgtttttt ttcggcatac
    11761 tattaaaaaa taatattttt taaaccaggc attttctctc cccccaaata agcaagcatt
    11821 gccccacccg cagtcgacac ccagtctaca tatgattcca tctttgtcat cttcaaagcc
    11881 ttaaccgttt cccctcctcc aaccaaacca aaagcgcgtc cagtagaaca agcggcaata
    11941 atagtgccaa tagccatggt cccgtgttta tagggagtat cctcaaattt tcctaaaggg
    12001 ccgttccaaa ctattgtctt cgcctttttc aaatatgaac taaaaagagc gatgctgtcc
    12061 gggcctatat caaaaataca atcactcttt tttacttccg acggcaagca gcgccgaatt
    12121 ttcccctgcg cggaggcgac aattacgtcg accggtagaa taattttggg taacaatttt
    12181 ttatctttta aaaattgact taaatatcca gccgattcct catcgcataa agattttcct
    12241 atttccagtc cctgatactt ccaaaaagta ttagccaggc caccaccaat aataatctgg
    12301 gcggaacgag catataattt tttaatcaaa ggcgctttcg tgcttatctt tgccccaccc
    12361 atcactacca ctaaaggctt gaccggtttc aatatttttt gcagattctt taattcttcc
    12421 tccaactgca agccggcaaa ggccggcaaa tatcgagtaa ttgcactcac cgaagcttga
    12481 gcgcggtggc agacagaaaa agcttcgttc acataaacat cagccaaaga tgccaactct
    12541 ttagcaaaag acgagtcatc tttcttttct ccgggatgag agcgtaaatt ctccaaaaac
    12601 actatttctc cttcctgtaa attcgccacc gctttctgtg ctttaggacc aagcaagtcg
    12661 ggaacaaatt ttaccggcgc cgccaataat ttttgcaaac gcctagccaa cggcttggta
    12721 gacaaggcct gatcactagt cttgggatct ccccaatggc taattataag cagacgagcg
    12781 ccatgctcac gcaaatactt gatagtgggt aaagcggcgg taatccgata atcttcacca
    12841 atccggccgt tcgaaacggg aacgttaaaa tcaacgcgca acaatactgt ttgcccctgc
    12901 cattttttct tctttttcaa gcttcttatt ttcataatat tattctcttt ttcccaaaat
    12961 aacttcggtc tgcaggctat tcccctgacg agtataatct atacttaaac tatcgccagg
    13021 ctgataggag gaaataatca cagccaaatc attttcggaa tttaattcaa taccgttaac
    13081 tcgattaata atatcaccgg ccattaatcc cgctttctcg gccggactat ttttaaccac
    13141 ggccggaaga ttgttgattc cctgcagttt tgctccttta gctggtaaat cgcttgcccc
    13201 cactacggca gataggttta gataatgaac gcccaaagaa ggccaatata attttttttc
    13261 cttcagcagg gactgccaat agacagataa ttccggagaa gtgagccagc ggccgtgatt
    13321 gtcgatagcg ccgatgattt caccggaaag attaaaaaca aaaaatggtt gtgcttgaat
    13381 ctgataagtc aagctaaatg tccgtgcgta atcatcacta gataaaataa cgtcatcaaa
    13441 attttttcca cttaaataat ttagtaaaaa aatattattg ccaccagcta gcaataaaga
    13501 ttgcccggat ttaatttctt catccgggac taaacgacgc acactaaacc cagaagcatc
    13561 aagcaggtga ataaaaacaa aattaccaat tgtgtccgtc ttggcgacca cttgattaat
    13621 cttataaatt ttacgactac tatcaataac aacaaaatcc tgaggaattc tctccagtac
    13681 gctctttgat aattcttcgg gccaggcagc cattacccaa ccatcgctag tagccactaa
    13741 agccgaggca aaaggctgct ctaacttata gtaagaactc tcatcttcgg cccggacaaa
    13801 aaccccgagc aggctagatt ttaaactacg aatagtttca tcggtcttca cgtcctgatt
    13861 cactacaact tttttagcat cacggataac caagcttggg ctcaaataac cataattact
    13921 taaattaagt tcacgattca gggcaaaaga actatcgtta gcagcataaa aattggcaaa
    13981 aagcgcacca gcggcgccag ctaaaagtcc aaaaaaggtg gcaattaaaa ttaggggcaa
    14041 ataattattt ttcatatttt tagttaaagc cagcgggcgc ttaataaaat gatggccaag
    14101 ctgacagccg taaaccaagc ataatttcta atcttatgcc tttttaaatt attctctaaa
    14161 taaagacgga caaaattgat taccgagtaa taggctaaag ctgataatat tccggaaata
    14221 agataattca acggcaataa agtaatcatc atggtaatct cggcggtaaa aaaagcaatt
    14281 aaaggccaaa taatctgatt gtgtttattt tgccagcctt gaataatagc cagcgcttca
    14341 gtattaataa tcaataaaac caagaaaacc actaacaacg gccaagggga aagagttaaa
    14401 aaagattgca agccgaacaa tgaagcggcc gcaaaaaaag aagccgctaa ggagctaaca
    14461 aaagcaatag gaataaaata atcattctgc ttaatggcaa caaatttctt aacgaacaac
    14521 agataacgat aaagatgcca aagcataatc agccatagaa ggcttagtat ccaaggagag
    14581 gcgattaagg aaataaataa atagaaacta acaatcaaca cagccaaatt aacataaata
    14641 gcgaccgaaa gccaagataa acgcctttcg gtagcttcgt agacagaggg gattaataaa
    14701 atacaagcgg caagcacgac tatccataaa gaagatgggt aaattacggc aatgcgccaa
    14761 cccaaataaa ataaaatagg agttagccaa aaataccaat taaataatag agctaaattt
    14821 ttcttcaaca taaattaaaa ttaaggtatc agcgattcag acatattttc aggcgtctct
    14881 gaagtggatg aagctgtttc ttcgggggta attatatctc cctcaaacgg aagactactg
    14941 gtggccgatt ctaaacgatt agaatcgcct gattcttcaa caaaaatcgt tgccggctct
    15001 atcggagccg aaggcaaaga cggtcccagg ggcgacggct tgactgccgg agattcttca
    15061 atattattga cggtattgat aatattctcg ctgggcaaag attcgacttc aaaatcagcc
    15121 gccgtgtcgt ctatcagctt taccgattgt aaagattgaa aatcgcttaa acgattaacc
    15181 ttcaaatact tattagaaaa agtataaaga gcatctttaa tatacagtat tctctgcacg
    15241 gaattatcat agcaataggc accacaccaa taatcggaac gctgatattt tcctccgtca
    15301 gaatgatcaa tctgcgactt taaaataaat ttaccctgct cgacggcaaa aactaaagca
    15361 ccactgaaat aaacgcgccc cctgtctaaa gacgaagtaa gtgaggcggg aatgactaat
    15421 aaattcttat cacggctgaa aagaaaagct ttatgattaa ataaagctaa agaattactg
    15481 ccggcgccgc cagcaacata agaatcaagt tcgcggggat tagtcggatt actaacgtca
    15541 aataaagata acttcaaacc gcctatttgc acattaccat aggcgtcaac ggtggtgtct
    15601 tggcctaaac caattaaagt attatcgtca taaggatgaa gatattgaga aaaaccaggg
    15661 actttaagct ctcccaaaaa tttcggcgcc ttaacatcgc tgagatcaat aatgaataaa
    15721 ggatcggtct gcttaaaagt gacaagatag gcgcgcttac ctaaaaagcg aacggaatag
    15781 attctttcgc ccggcgctaa attttccact tggccaagca attgtaagtc ggcggataaa
    15841 acataaacgt tggaataaga atcatcgcca ccagctagag tgctgaaatt acggctacgc
    15901 gtcgtcgcta agcgcaaatt tccatcagca tcttcatcca aagaaaagtg attcaaaacc
    15961 gctcccggca ctgaaccggt agcgcgataa acaggcttac caccgtttaa agctaatttg
    16021 taaataatag ttctctccca attttgtgac tgattttcat actcttgctt tagggcggcg
    16081 gcaatctctg attccaaaga gactaattcc gctgacgatt gcgcgttcaa aaaattttga
    16141 aacagttgta gaagcttctg ttttttctct gacgggctta aaatatcact atcagtctga
    16201 ttgattttct cgactcgagc ttgttccgcg ctgctcaatt ttgcaccaag tacgtttttc
    16261 attaccgcca agcgcaaatc agactcatca agatgctgag tataggtgat aaaaatattt
    16321 ttcgccgaaa cgtaaatggt ttgagcgccg ttaagaatat aggtttgagc ggcgaccgga
    16381 gcatcgttag cgcgcaaatc caaagtattg attgagataa aattatagga atcgtaaggc
    16441 aaatcaaagt aataaacgtc aggagcaaaa caacgatcag cgcttgaaca atcattagta
    16501 agaattttgc caccatcaac tagacgcggc accaccctct ctccaggaat ataagtatta
    16561 taattatcaa taatcaaata taagcggccg ttaattaccc gagaatcgcg ataggaaccc
    16621 tcaaaatcca aatccctaat ttgcttcggg ctggtcggat tagataaatc aaaaatcttc
    16681 acgaaagtat aaggtgattg gcgacgaaaa ttttgataaa cactgctctc cattaattga
    16741 ttgtcagcac cgataactac tagatgttga ccatcaagat atatattact aggtcgtgaa
    16801 gtgaaagata tttttgccac tgcccgcgtc tcttcagccg gatcggcttg aataatataa
    16861 aggtcttgat aaaccaccgc atagatataa cgaccatcag ttttaataat atcagcttca
    16921 tcaactccct caacctgaac gttggtctga gaataatcac tgtcagcaga agaatttttt
    16981 accctctggg acgaactttc atgaaaaact gcgtcattag cggcgacggc tgaatcgata
    17041 ctcggagttg ctaagccgcc tcgataagat ctcagtgttt ccacggcatg agtgtcaaga
    17101 aatgatttta attcttgata atcggagaat ttcttcatct gaccggaagc ggaaatttcg
    17161 gcttgattgt caataataat gacattatct tcaatattct ccgctggcgc cttctttgac
    17221 cacggcaaag aacagccgcc ggccaataaa gcgagaaatg gaataaaaat aaggaaataa
    17281 tagcgttttt tcataaaagc tttttcaatc caacagaaaa gcagctgatt attttaaata
    17341 tataaggata tatttatata ataatatata ataaatcttt acacaaactc aaaaactagg
    17401 gtattaaaac tgtcgtgccc accggaatgc gatcatatat ataagaagcg gcatcaaggc
    17461 ctaagcgaat acatccgtgc gaaaccggcg taccgagatg attttcacct tcacgataac
    17521 cgtttggcca aattggcaac tcatggatgc caatactgcc ggcgtttaat ccaagccaat
    17581 aaggcatcca tagtccataa gtttgagacc aggccttctt gatcttatta ttcactttaa
    17641 actctccggt cggcgtgggc gtgctcgcct tgccgctaga aatcgggaaa cttttccaaa
    17701 cataaccgtt taaaagataa tctaaagtct gagaagataa atttacccgc aaagatacat
    17761 ccaggcgcgc cccttcggct agagccggat taaaaccata atcagcctct tccccgtctt
    17821 taaaaccgtc gccgtcggta tcagctaaaa ctggactagt tctaaatttt atctcccagg
    17881 catcgcttaa accgtcactg tccatatcac tgtcaattaa tctcaccggc tcgggattat
    17941 gtggtgaaaa accgcggtta atttcttcaa aatcgctata accgtcgccg tcggtatcga
    18001 cggcatacat gtcggtataa tatacttcct gttcatcttt atccggtacg ccgtctctat
    18061 cagcgtccgg taaaccgttg gcctgaacgc cagtaaccgc taaaagcagg aataagaaga
    18121 aaaatgtaaa atttaatatt ttcatgcgta tactttaatt tatttgcgaa agctgaactc
    18181 acagccgcag taacgttgta aatacaagcc tctttcccgc gcccaatcca aggaagcgcg
    18241 gaagccgtta tccgcctgaa aatcctgatt aagaaaaacc ggaccgccct cagccgctaa
    18301 ttctcggcaa atttttcgaa tcgcctctcc gtccttatac ggactaacca ataagctggt
    18361 gctaaaatag cggaaaccgc gacgcttggc aaaatcataa gcggcttgca gtctttctcg
    18421 ataacataac tgacagcgag cgcctctttc cgcatcgcgc tccctgtctc ttactagctc
    18481 tcgccaagct tcatggcgat agggcacagt gattaaatcg actttcaaaa tatccgccgc
    18541 tcggcgcacg gcctgtagac ggcgctcata ttcctgaaga gaagatagat tgggattata
    18601 aaaataccaa gtgacgcggt aatgcggcat taattttgtc tgggtagaat aagaagcgca
    18661 aggagcacag caaatatgaa acaataaacg gggacgacgc gccctctccc accaagaaaa
    18721 aaagataatc aaaagaagca ttatcactaa aagaataagg agggcgaata gctttccccg
    18781 gctttgcaaa aagaggccgc ttaaaccgaa aacagccgca aaaacataaa aaacggcgac
    18841 tgtctgccgc gacgacaagc ctaaatcaag caaacgatga tgcagatgct gacgatcggc
    18901 aaaacggaaa ggattcctgc ctttaagtaa acgcctgata atcgtccaaa atacatctaa
    18961 aatcggcaaa cccataatca ataaagcgat tgctattttg ccaccagaaa taaccgccaa
    19021 aacccctaaa acatatccga ttaaaagcga gccaccttcg cctaaaaata ttttagccgg
    19081 attgaaattt aaaattaaaa aacctaaaca agcgccgcta aaaatcaaag cggcgatagc
    19141 aatatccggt tgggaatatt gagtagttga agtaaacaga aaaattacca aagaaccgat
    19201 gagacccaaa ccgctaacca aaccatccac gccgtctaaa agcttagtgg tgtacatcat
    19261 tcctaaaagc cacaaaacta taaaaacggc gcttaaaaca gcgggtatta ttataaaccc
    19321 gccgaaggga ttggacatct ttgtgatgtc aacgccgccc acaatcggcg ccaaggccgc
    19381 caacacggga aaaataatct gccagtgcgg cgccagatta tatttatcat ccaaaaaacc
    19441 gccaatcatt agaattaaag cgccagcgaa aaaacccagc caatgatgta tttctaaatc
    19501 gcctgataaa atctgagaac tggcaaaaaa gagaataata aaaaaagtta aaaaaatcgc
    19561 cacccctccc aacaaagctg tttctttatg atgtaattta cgcacgccat ccggacaatc
    19621 tgtaatcttt aaaaaaagag cgccgcgacg aacaagcaag cttaaaataa aggacaaaat
    19681 agtggcagcc aggaaaaagc ctaaatttaa caacataatt taataagatg taatcttgat
    19741 attaaaataa atttgggtta atatctagtt attgttcaat aaaaatattt gaaaaaatga
    19801 agaaaaaagc agaagaaagg atcagtcctt ttatgaaagg caacctgagc aatgacgaat
    19861 tagaagaagt agttagagct ctttctcctc aagacttagg ggaaataaaa caactatttc
    19921 ttttaaaaat taaagaaatg gagagcaacc aagaagctct taaaaaacgc ctaaagcggg
    19981 cggaatgccc cgaaaaaata ataaaggaaa gacttgccct cacagaggcc aacattaatg
    20041 aagttagaag gtgttggcac atctttaaca atatttttga agagcgaaaa agcaaaaagc
    20101 tttaaaagcg gccactcttt aaaatattgt aaaaacacaa aatcaatcct catcaatcct
    20161 cgtaccgaca taacggtgcg aggtttttta tttttctctc attttaaagg gcacgacctt
    20221 tatccacgcc gatattggct tgcgcgtcaa ttaccaccgt atcgcgacgc ttcaattctc
    20281 cggctaatat cttattggca atgctatctt caaccctgtc ctgtaataag cggcgcaaag
    20341 ggcgggcgcc aaatttagga tcataaccag cttgagcaag tttcatcact cctttcctat
    20401 ccgctttcaa attaatgcct ttttccgcta aacgcttctt gataccttta agcataaggg
    20461 tggtaatctg aaaaatattt tcttcggtaa gtggtttgaa cacgataatg ccgtcgaaac
    20521 gattgattaa ttccgggcgc atgaacttat tcaactgatt atctatcaat tcctgtttaa
    20581 ttagtccgat atcggtcttg gctttgattt gttcttgaat ataaacagcc ccaatattgg
    20641 aagtggcgat aatgatagat tccgtaaaac ttatcgtccg accctgtcca tcggtaagac
    20701 ggccgtcatc aagcaactgc aaaaaaagat tcaaaatatc tggatgggct ttttcgattt
    20761 catctaacaa aattaaagaa aaaggtttct tacgcacggc ttccgtcaga taaccgagag
    20821 tgccgtcgac gtcaccaatc attttacgaa tactatccgc cgcctgatat tcactcatat
    20881 ccaagcgaat catataattt tcatcaccga aatacacttc ggaaacagtt ttggccaact
    20941 cagttttacc gacgccggtc ggacctaaga aaagaaagct ggcaattgga cgcttagcgt
    21001 ctttgagttg agcgcgcgct cggcggagac tggaagccac ggcgctgaca gcttcatcct
    21061 gaccaatcat ccgctgatga attctctctt ctaaagcgag taatctttgg ccctcgctct
    21121 cggttaattt ttgagtggga attccggtca tttcgctaat agcggccgcc acctgttctt
    21181 tggagcagat agcttggcgc gggtcgcgtt tcttggcctt agccgcgatc atcgccgcat
    21241 tttgcaaaag agtaatcgct ttatctggta aatatttatc gtgtaaatag cgggaagaca
    21301 tgactaccgc ctgttcaatt gcattataag taatgaaaat tttatattta ttttcaaacc
    21361 aagcaacttt actttccaac atttgaatag cctgattaac ggacggctct ttaatgccaa
    21421 cagttgtcat cgcttcgcct aaagctgtct tttctaaaat acgagaataa ttttcactat
    21481 taatagtagc taggcaaaaa atcatcttcc tgactaaagc ttcggctaac acctccgaaa
    21541 gatctaagct tccttccccg ccagaactaa ttccaactaa attttcaata ttagctatat
    21601 gcaaaacgat gttaccggcg cgagccgtct cattaataca agtcagaagt ctttcttggg
    21661 catcagcggc ggtggcgccg ctcaccagac gggaaacatc tatttccacc aaacgcttat
    21721 cctgtaaaaa ttttggcact ctttcttcaa ccattaactg agcaacgcca ttaattacca
    21781 tcgttttacc ggtaccgctg gcgccaacca gcaaaatacc agcctggccg ctttccatcg
    21841 cttcaaagat ggaattaatt tctactttcc gaccaataca aagttctaag cgtccatatt
    21901 tagccgctaa agttaaatcg tgggaaaaat gatcgagggt cggggtggcc actgccgtat
    21961 aagccctatc catgccgctt cccggcttca aaccggctaa gcgccgatag gagtgataac
    22021 tggcgactaa ttgacggtta atatgaaacc attcacaaac attatcaatc ttatcggcgt
    22081 ctaattctaa atcatataaa atttctgcca gcaattcatt gcgctgataa caaaaagcta
    22141 aggcatttaa cggctctaag ctatcctgat ggcgggaaat agcatcagcg caagcgtcaa
    22201 tcataactgc gattaagtta tctgaaatta aaggtcgacg attttcgcct tccgggcctg
    22261 ataaatgcct atctattttg gtcactaaag aagtggcatt aataccgagg cgaataaaaa
    22321 cattttgaac agtgatatca gtcaataaag cgcgaaagaa atgcataacc gaaacttcag
    22381 gttgctttaa tttagcggca atgagaaaag atttttctaa aacagcttcc atctcagcgc
    22441 taatcgtagt ggcgatatta taatatggtt tattattcac tttactaagc caataacgat
    22501 aattaatctt tttcttggcc ttctttttct ggctttggcg atagagtaaa aacaaatcaa
    22561 accaagtaga taaaagaaat attaggatta aaggatgctt attttcccaa aataataaaa
    22621 ttttaagagg cgaaacggtt aaaattgttc tttgctcaaa tagccataca actaaggcta
    22681 accaaccgac aagagcgacg ataaaaacca ctatatctaa gattttgtcc gcccggcggc
    22741 gccaagcggc tacagcaata agggaacggc taaaattttt ccgccaataa agaaaccgcc
    22801 cttgaaacca agcgccctga ccatattcct tgtctatttc tatttttaaa ctggacggat
    22861 tatccatata tatttaggcg ggtaaaagtt gaaaagccgt cgccgcgatc aataataaag
    22921 gcagggcgat ccagacgacc atcaaagcaa gaccgatagc tatccagaaa cacaaaatca
    22981 aacctcgaaa aattatttgt atcaagcgaa taaaaaaact aataatacgg ccggcaaaat
    23041 catgctgtcc gtacatcggc acaaatatat ttttgagcca aaccaaaaaa cctagagatt
    23101 tttgttcgtc ccgccaaaaa gcaataattc tagaggcaaa catgataaag ccaaggctat
    23161 accaccataa agggaaaaat aaaatatttc ccaagaaact taagactgct tgtggcacag
    23221 aagttaaaaa atgagactta gccatatatt taattatagc attaaaaagc agctctcgcc
    23281 aaatcaaata acctcctccc cgccctcaaa aagtcaccct ttcagggatg acttttcaga
    23341 tggggttttc tatgatttcg tgaagtactc aagtctccgc ttacactcta ggaataattc
    23401 atccccgccc aagctgctag cgagtgcctg gtttttacca tagtaatagt gaaaaaaata
    23461 gtattaataa cctaaataga tagagagatt gacaagatga gatcaatata ttatgatata
    23521 ttttagcagt caaagactaa gagtgctaaa ttaaaaatta taaaaaaata ttatatatat
    23581 gtttaataaa tttacagaaa aatcccagga agctattatt aatgcgcaag ccatcgccgc
    23641 caatttcgga caaccgaaca ttgaagcatt acacattctt ctctctctat tacaacaatc
    23701 agaaagcctg atcaagccga ttttagaaaa aatgaaaata gatccagaag tattagaaaa
    23761 ccaaaccatt aatgaaatta aaaaactgcc aaaaagcaaa gtcgacttac aaaatggtcc
    23821 cattcaagca gtttccggca ctaacgaaac cgcccttatc ttggagcgct ctaaaaaaga
    23881 ggccgacggc atgggagacg aatttatttc aaccgaacat atcctgcttg ccttaatagg
    23941 aattagctct cctgcgcaga ctattctttt taactttaat gttacctatc aaacagtcgt
    24001 atcaatcctt caggaactgc gcggcgatga gcgcatcacc gacccttcac ctgaaggtaa
    24061 atttaaagtt ctagagaaat atacgagcaa tttgacagaa ttagcgcgcc aagaaaaact
    24121 tgacccggtt gtcggccgag atatggagat caggcgagtc atgcaagtac tcttacgccg
    24181 gacaaaaaat aatcctgtcc tcatcggtga agctggcact ggaaaaactg ctatcgttga
    24241 aggtttggct caacgtattg tcgccggcga tgtgccggaa aatttaaaga acaaagaaat
    24301 cgttactctt gacttgggcg ccctggtcgc gggtgctaaa ttccgaggcg aatttgaaga
    24361 ccgtttaaaa gccgttctga aagaagtcca acatcaaaat ggacgcatca ttctttttat
    24421 agacgaactc catactctga ttggcgctgg ctcttccgaa ggctcaatgg atgcctccaa
    24481 catgctcaaa ccggctctag ccagaggtga attaagaact atcggcgcta ccaccactaa
    24541 agaatatcaa aaatatatcg aaaaagatgc cgctttagaa aggcgcttcc agcccatcat
    24601 ggttaacgag cctagcgtcg aagatacaat cagcatgtta cgaggcttga aagaaaaata
    24661 tgaagtccac cacggcgtcc gcatcaccga tgatgcccta accgccgccg ccaaactttc
    24721 caaccgctat atcagcgatc gcttccttcc cgacaaagct gtagatttaa ttgacgaagc
    24781 tacatcttct ttaagaatgg aaattgattc aatgccggac gaactagacc aattaaaacg
    24841 acgcatcaaa caactggaga tcaacaaagc tgcccttctt tcttcggaaa aaaaatcacc
    24901 aaaactgaga gtcataaatc aagagttgag caaattaaaa gagaggtcaa atcaattgga
    24961 gttgcactgg cttaatgaaa aaaatattat ttcccaaatt cgccagcata aaaaagaaat
    25021 tgatgaatta aaaattaagg cggaaataag ccagcgacaa ggagctgatt taactgaagt
    25081 agctaagatt aaatatggtt tgattccgga gctggaagca aaaataaaag ccagccaaga
    25141 cgaactgatt agaatccaaa aaaaaggtca gcgcattctt aaagaagaaa ttggggaaga
    25201 ggatatagct aaagtagtag cccgctggac tggtatcccg gttaacaaga tgttagctga
    25261 tgatttattg aaattagcta aagccgagga agaattaaca caagaagtaa ttggccagga
    25321 agaagccatc gcggcggtgg ctaaagccat ccgccgttcc cgcgctggaa ttaatgaaga
    25381 aaaaaaacca ataggctctt ttctgtttat cgggccaacc ggagttggta aaaccgaact
    25441 agctaaagcc ttggctagat ttctatttaa tgatgaaaat tctctgattc gcttggatat
    25501 gagcgaatac atggaaaaac attctgtcgc caagctcatc ggctctcctc caggctatat
    25561 cggctatgac gaaggtggac agctaaccga aaaagtcaga cgacacccct atagcgtcct
    25621 gctatttgac gaaatagaga aagcccatcc ggatatgttt aatatgcttt tgcaaattct
    25681 agatgacgga cgcctgacgg actcaaaagg cagagtagtc aactttaaga acacagttat
    25741 cattatgacc tctaatctag gcaatgaagt catcaaacaa tatgctatcg gttttggaga
    25801 agaaaatgaa cagcccaaga aagatcaagc cagaaaccgg gaaatgagag acaaaataga
    25861 taagatatta aaagagcatt tcaaattaga gtttctcaac cgcattgatg aaattgtcct
    25921 ctttaagagt ctgcgaccgg aagatttgga aaaaattgtt tctttagaga tgaataaagt
    25981 agaaaaaaga cttaatgcca aaaatatcaa gttacaaata agcgctaagg ttaagaagat
    26041 tctggcggaa aaaggttatg acgccagtta cggcgctcgc cccttgaaga gagttatcca
    26101 aaatatgatt ttagacgaat tagccatgca aatcatcgaa aacaaaattc aagaaggcga
    26161 taaaataagg atagacgtcg actctgaggc aaaaataaaa attaaagtcg aggcccaaaa
    26221 taaaagtaaa taaaaatacc gacaaacagt cgccgatcaa atagccggcg gctgttttag
    26281 tattaaataa ataaattacc cgacaagctt ggtatcttca aaaaaatata ttataatgtg
    26341 aatactctaa actaagtaat atgaaaaaac acttaaacat tcttgccgcc gttatctgta
    26401 ttttctttat cataccggct gctggagcac aagatattga ccctggtttt aaccccaact
    26461 ttattatttc tgatgaagaa atgatggatt acgatagcct gagcctggct caaatccaaa
    26521 atttccttca gaagaaaaat agttatctag ctaactacag caccacaagt gctcatggta
    26581 cgcaaaaatc ggcggcagaa attatttatg atgccaccaa taataactat gattgtagcg
    26641 gcattacttt aagcgacagc cccacggaag cggaaaaaca actaaaatgc cgtaaaatta
    26701 cgaccatcaa ccctaaattc ttgttagtcc ttctccaaaa agaatccagt ttaattgaag
    26761 atccgaatcc caatcaagcc cgtttggact gggcgaccgg ctacggttgc cctgataatt
    26821 ggacctgtaa tccatattac aaaggttttg gcaaacagat aaacagtgcc tccctgcaat
    26881 ttttggccta catgaaggaa ccacaaaact ataactataa agccggacag acatacacct
    26941 tcagcaaccc ttacggtact atctccaaca aaaccataac cgtcatccct gctaatcagg
    27001 caaccgccgc tctctataat tatacccctc acgtctttga cggaaattat aatatctata
    27061 aactgtacca gcgatatttt cccagtactc ccgcccgcta tcctgatgga tccctgttac
    27121 aggtcaaagg cgaagctggt gtctggctaa ttcaagacgg acttaagcgc ccttttctca
    27181 ctaaaagtgc tctgctttcc cgctatagtt tagataaaat tattatggtc gaagccagtg
    27241 atttagaaag ttacgacaag ggcaacccca tcagtctgcc taattattct atagttaaag
    27301 cccccgataa cactctctat ttaattgttg gcgacgaaaa aagacaattt gaaaatcaaa
    27361 ctgcgtttaa aagctttggc tttaatccgg aagaaattat tgccgccagt actcaagacc
    27421 tgagctatta ccgcaacggc gccatgctta ccgccaattc cgtttatcca accggcgctc
    27481 tgctccgcga cccgcaaagc ggaggagtct attatgttca agatggcaat aaagcgccaa
    27541 tcattgataa agtcttactg agtactaaat ttaagggcaa aacagtccaa aaagccagcg
    27601 cggaagaatt ggctcgctat accaaaatca gcccggtttt atttgatgac ggtgaattgc
    27661 taaaatcaga gagctccagt accgtttacc taattgccaa cggcaagaaa aggcctttct
    27721 taagcggaga tgattttgaa cgactcggct acaaatggtc aaacatcatt accgtatcgc
    27781 cacaattact atacttatat ccactcggag aagctataac ctacaaatcc gccgcttaaa
    27841 taaaataccg aatataaaaa acgtcttcct atgaataaaa cgaaggcgtt tttatataca
    27901 acaaaaactt atataagtca atatttaaga aaaaagaaaa ttatattata ctaaaattgc
    27961 tataaattct aataacataa tctaaaaatt taatcatgcc tattgttagc gcctctttct
    28021 tgccaccatc accgctatta atccctgaaa ttggcaagca aaacaatcag atgctccaaa
    28081 aaactagcca agcctatcgt caaatggccg ctatttttga gaaggaagaa atagaaataa
    28141 tcatagtaat ttctccctat ggactttccc aaaacgaaaa tattagtctt aatatcgcgc
    28201 cgcagctaca aataagtttt caagaatttg gatatctagc caccattaaa actttaaatc
    28261 cagcttggcg tctggccgat gctattgtta aggagatggg acacgactac ccggttaaac
    28321 tcagcagcca agaaaaatta gactacggta gcgccgtgcc cttggccctg cttacggaaa
    28381 atataataat aaaaacgttg ccgcttttcc ccgctaatca gaaaaaccgc gcctatcatt
    28441 atgaattcgg actcaaactt ggaaagattc ttaaaaaaag accggaaaaa atcgctttag
    28501 ttgcttctgg agaactgtca cgatgcttaa aaaagaactc ccccggaggc tattcaccca
    28561 agggagcacg ttttgataac cgcgttatcg aatatttaaa tgattccgaa aacggcctta
    28621 ataaaatatt ggcactagaa gataaaatcg gcgaagaggc caaagaaggc ggattaaaac
    28681 aattaagcct cctcatcggc gctatcggcg aaaactgtca ggcacagata ctggcctatc
    28741 aaaatgattt cggcattggt tacctaagcg ttaattttaa cctgcaaatc gccactattt
    28801 agaaactaat atggaaaata ttgccgatct catcagccag cgtcaacagc aaaaagcacc
    28861 ggcctatccc tggcaagagc tggccctgag aattattaaa gagctatcga ttcctggctt
    28921 taaaagaagc gccgttttta aaatctgtaa ggaactaagt cccgtcttgg tagaaagggc
    28981 actaaacgac actaaagaac tttgccgaaa cggagtcaga tggaaatatt tctttaagat
    29041 tgccgatcaa tatttagcgg tgaagaataa gaataataaa aaatataaaa aagaaaaaaa
    29101 tgaaaaatat caaaggtaaa ataatggaga tcgtctacca cccacaccca atattgcgtc
    29161 gccaagccga tccggtcaag gtagaaaaaa ttaaaagtcc ggaattcaaa cagtttttag
    29221 cagacatgga agcgacaatg ttggctaaag atggcgctgg tttagccgcc cctcaagtcg
    29281 gcatcagcga aaaaatcgta gttattgccg gcaagaacgg gaataatcta attcttatca
    29341 atcctcagat tactcaccgt tcttggggaa aaataattag tgaagaaggt tgtctctcca
    29401 taattgacaa cagggggcga attatctacg gaaaagtaga aagacataaa cacgtaacct
    29461 gtcaatatat agacgcccag gggaaaaaga aaaaaattca agcagaagaa ccgctggcgc
    29521 gagtaattca acacgaactt gaccatttag atggcatcct ttttattgat cgtttaattg
    29581 attaaaaaac cggcctaatc gccgattttt ttattgtttc taaattttat tattttcttt
    29641 cccgataacg ccaaaaaaaa tataaggcga taacaacccc aaaggctact aaagcccaag
    29701 aaatttctag ataatatctc ttccacgccg cttcattagc gccaaaaaaa taacccagag
    29761 cagctaaaat cagagtccac agagctgatc ctaaaaaagt atagaaggaa aaacgccaca
    29821 aaggcatttt aacgaatccg gccggcagag aaattaattg ccggacaacc ggcactaaac
    29881 gcccaaaaaa agttgaacca ctatcatatc tagaaaaaaa catttctgct cgctcaattt
    29941 gcgccggcct tattagcaaa aaacgagcca ccttagtttg agccaaacga taaattaaag
    30001 gtcggcctaa ataataagct aaaaaataat taataaaagc gccgatcaaa ctaccagcca
    30061 cacctgctaa aataactaac cagatattca aactaccttg agcagctaaa taggcggccg
    30121 gaggcaccac cacctctgag ggaaacggga tgaaagaact ttccaaagtc attaaaaata
    30181 ccacccccca ataaccgatt tcttgggcaa aatttaataa ataattaagc aaggtaatta
    30241 acataataaa tcatgtacta ataattaatg atacttgtct ctttagtata cagtaaaaat
    30301 aaaaaaactc aatccagcaa aacttgcttt tgccttaata atcagctaat ttataaacat
    30361 gacagccaaa ataagaacaa tttttatggg cacgccgaaa tttgccgttc ccggtttaca
    30421 agctttatta gaaagccctg attttaacat tgtcggcgtc tatactcaac cggataaacc
    30481 ggtaggacgc aaacaaatac tgacgccacc gccgattaaa gccctggctg ttaagaataa
    30541 tattcctata tttcagccaa aaaaaatcaa aacagaaacc gaaaatatta aaaatttaaa
    30601 accagattta atagtagtaa ttgcctatgg tcaaattatt cctcaggcaa ttttagatat
    30661 tcctcaatat gggtgtatta atgtccacgc ttcccttttg cctaaatatc gaggcgcctc
    30721 ctgcttagcc gcgccaatac taaatggcga tgaaaaaagc ggcatcacta tcatgaaaat
    30781 ggatgctggc atggataccg gtgatatcat caaacaaata agcgtaaaac tgtcagataa
    30841 agaaacgcta aacagtttgc atgaccgcct atcgatatta ggcgcagaaa tcctaaccga
    30901 cactttaaaa aaatatatta atggggaaat aaaaacccaa aagcaagatg aaagcaaagc
    30961 cagctacgtc tctttaatta aaaaagaaaa tggccggctt caagtagatt taagcgcaac
    31021 cgcactagaa agaaaagtcc gcgcttataa tccctggcca ggcgctttct tgagacttaa
    31081 taatggggaa aaaataaaaa tcatcgaagc ccaagcggaa aaagccgttc atcaagggaa
    31141 aattggcgaa atatacataa aaaatggaca cttggcctta aattgtggac aagatatttt
    31201 gtttatatta aaattacagc gtgaaaatcg gaaagtgatg aacgcctccg actttttaaa
    31261 aggcaatcaa gacattctaa atttaaccgc catctaaaaa ggtctcttcg tctaacggct
    31321 aggacgccag gttctcatcc tggtaatagg ggttcgattc ccctagggac tacataaata
    31381 aacttttgag aaatttcaga ttttaagact acaaaataaa aagagcatac tgaaagtg
//
LOCUS       DAOQSA010000007        30950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_864_length_30950_cov_8.704548, whole genome shotgun sequence.
ACCESSION   DAOQSA010000007 DAOQSA010000000
VERSION     DAOQSA010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 30950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30950
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_864_length_30950_cov_8.704548"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="PKK65_01340"
     rRNA            complement(<1..776)
                     /locus_tag="PKK65_01340"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1124..1297
                     /locus_tag="PKK65_01345"
     CDS             1124..1297
                     /locus_tag="PKK65_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81359.1"
                     /translation="MEDSKQKAIAEIEDIFSRAKESLLSLHNQKMALIKKFKDAEDAA
                     QAADILDKLKQLN"
     gene            1314..5696
                     /locus_tag="PKK65_01350"
     CDS             1314..5696
                     /locus_tag="PKK65_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81360.1"
                     /translation="MSTEKLKVKEEESLASLLPDLAKIKQWQKDINAVKGDKIKTAEL
                     RRKFKNIFGVPFGQKLVADLIRLEKGEGKEKEKEENKEKPVQPLVAVVEGAKTLQADV
                     HEQQSLEVAMAQTIINPELNKQTLYIDEKKKEVVANAIDGAEDVALKASKEAEEGETT
                     VDNQTNGELKKEDEMDRVDEEALRQEILSTKEAVLAEINAGYQAERKAHNFELEEKIR
                     QADSFDSLLNIIKESEGIQGSKEFFSPEMLFNVLTSIRSGEAPLSAATSGLGFRETVE
                     RLLSSEHKIMEAEVSKCNSLEDFISLLNKFPELAVLGGDIKNINEYISYFSREVEFTE
                     QLYIADNFKNEEHLHNALNFATRRYGLRDKVTQYIIKKNGLHFKDDPAKKIREEVNDP
                     VNNGLEHVDGPKENKSESQKREKQIAPEALDYFYDLGLNDKDLLSIPGLTELNSVSQL
                     LIAKSLKNLALEKANRNVSKKQADASAVAKGFWGKLSTGISNAFKIGKQKKEAILEQA
                     HGGLAEHKVELTRLVDWAQNLDLKEIETEEGRFMADFTGHFDLKNINEKQAAIVKEMN
                     ENANWLASCAKEYRLFTYTPEPKGSKTHQEYYQAKRAYDDSRKQMSALLSNDLNWSEA
                     SVLQAINKMDANVNMVQFMSANPDLEKDWQEMIKNKSVLAKAFAKDNWKFFASGYAGR
                     MALGSVLGFVAIPAVAMAVGAWRGREKGRQALRNADKGLEKKDLPGDSPLLTERKMVL
                     KAIQELVPTEYAFNREEWLAKVANPEQKSLYYDLRVKFNDLDARWRQEEEKKKGEHNK
                     ERNQEYTSGKNIERKVLRAETLMSKLQNLIDKAQQAEGEKRAQLLFQLSRRVNFSKDL
                     VDKGLINFGSVEERSSRMLDFYQLLSQGQILQLDKDVQVALVAKGDYESVYEDVNIKE
                     LSKLNERAEDLLNSLEYFAEMQLDKNRRSFLKKKILQGAIVGGVFATSGALVRELSGS
                     WLEDKAAAGFRFISENTKFGAAVSDTISDIKNYISPNLNSLAGSSAVETATWSDQISN
                     EGLKAGQYDSVWLSTKNIFESHAAELGYQGDINDVAALRHWAEIQTNRTLADTGDITD
                     KVFVGNKVTLEKNGDNFIVRVEAGSGAQPGYLSETSIDLPSGKDGLQETPPNPDGSQP
                     IQTPDTFIDSKGVPNVGKVREIWADRAGARFGLSSEQVSYADENVIKAEIDGYDVFID
                     TQNNTFLFFDDNANEVSGLLVDDNGHLINNAEEFLRSKFFSEEASAAGAIDYLSNDNV
                     EQDASSSLPELEPSLPSSQTEALQSLNQSDIPNQFDNIINNYVRETTGGSIKEALEII
                     AARTKTPADKVLLEYIEENHISPDASLSDLLSSDAGLLMSMNSPENSVLTELYDLRHA
                     SSSQAQAEMLYKYLHTALSEDLKDAGKLAHFNKLASLNAFRVGSSLDGKVDTLVMQVG
                     DNVRKVDFSAKGISEVLKFVKQML"
     gene            5889..6839
                     /locus_tag="PKK65_01355"
     CDS             5889..6839
                     /locus_tag="PKK65_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81361.1"
                     /translation="MKMKKMFPVAILATFFVAFSIKAQPTVKNPSNGMSVELSYNGRQ
                     MILRPAESKAIYFLPPSGEARNVRLRYFVGEDIKTVFTTNLNIVSGQELVLPLSAPER
                     IRATVDKQSASQKDQSTTASTMPGFSPKIPEVSGVLEVKKFPLTLIDSTQKSILVFEG
                     DFYGAALAPNERTEPIITGPGIISMKILYDADPPASSTGKNLWQSPVQGIVTQGQEYF
                     VLRESHLRNLQRDVAKVVFFNPTPYSMVPDGLDIDPIPPNRRSKAVRLNQGFNNFSFV
                     YINEAGVKVRAVFELVVTKGTPVVRLNLNPLGNAYGVTGN"
     gene            7056..7703
                     /locus_tag="PKK65_01360"
     CDS             7056..7703
                     /locus_tag="PKK65_01360"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNU81362.1"
                     /translation="MILTWLGHSCFKLQDKIGSGGVTVVTDPFGKQFGLKLPNCEADI
                     VTISHNHEDHNNVAALRGNPFVINCAGEYDIKNVLIEGIDSFHDDVQGAERGNNLIFR
                     LEIDDISVVHLGDLGQPLDSGQLEKLAGTDILLIPVGGKYTLDAKKAVEVIAQIEPRI
                     IVPMHYQTDELKIEGLDGVDKFIKEIGLSPSYEEKLKISKKDLPVEDMELVILKY"
     gene            7739..8344
                     /locus_tag="PKK65_01365"
     CDS             7739..8344
                     /locus_tag="PKK65_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011763353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNU81363.1"
                     /translation="MITHPKKERTLVILKPDAIQRGLIGESIKRFEAAGLKLVALKLI
                     MAQEEVLWKHYNKDDAWFQKKGELTVKNRQDLGMPIEKEPIEYGKDIIRALVKFMSCG
                     PIAPMIWEGNQAVAVVKKIVGGTEPTTSDVGTIRGDLTLDSYELSNRDERAVRNLVHC
                     SDEVSEAVREINLWFKPEEILEYHLIAEQILYDVNLDGIKE"
     gene            8353..8886
                     /locus_tag="PKK65_01370"
     CDS             8353..8886
                     /locus_tag="PKK65_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81364.1"
                     /translation="MSKQEKNNQVAEEKKKRFFLKVGVWVFSVLILSFWLFTLVHSFN
                     NTTISVGQTIDADTAAWQADLNQTIDIIRNEMVDKTSVASNTPAVSQDFLEKMNANLE
                     EQVAASETAPIVEENITDKSRATITPEELISGLQKRLPELASSSCPEFIDCMPSIGEA
                     KPCVIPPGCENITQIAY"
     gene            8888..11416
                     /gene="gyrA"
                     /locus_tag="PKK65_01375"
     CDS             8888..11416
                     /gene="gyrA"
                     /locus_tag="PKK65_01375"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNU81365.1"
                     /translation="MKKENKDVKIGKEESNNNTILPNGRRQTDFGLIQDQPLVEEMSR
                     SYLEYAMSVIVSRALPDVRDGLKPVHRRILYAMWNIGLRANGKFRKSATVVGEVLGKY
                     HPHGDSAVYESMVRLAQDFAMRYPLVRGQGNFGSMDGDSAAAMRYTEAKLAGIAEEML
                     FDIDKDTVDFIPNYDGSQKEPRVLPAKLPALLLNGTMGIAVGMSTNIPPHNLGELTQA
                     ICHLLDNPEASIEDLMQFIKGPDFPTGGIIYNQKDILAAYATGKGSIVIRGKAEIEEQ
                     KGGSFQIVISEIPYQVNKADLVEKIADLVKEKKLDGIKALRDESDKDGVRIVAELKKD
                     AYPKKILNALYKHTALQTSFHVNMLALVDGIQPKVLTLKMILEEYIKHREEVVRRRTQ
                     FELNKAKDRSHILEGLLIALKNIDEVIKTIKASKDKEVAKANLIKKFKLSERQAIAIL
                     EMKLQSLANLERLKIEQEGKEKQKLIKELESILKSVAKIRGIIKEEIMAVADKFDNPR
                     RTAVVVHGVKEFSLEDLVPNEETMVMMTRDGYIKRLEPDTFKVQARGGKGVMGLTTKE
                     EDTVEFMFTTFTHSDILFFTTRGRAFQLKAYEIPQASRVAKGNSIVNFLQLTSGEQVT
                     SVLPLDKIFGSKYLFFATEKGLVKKVELSAFNNVRRSGLIAIKIKEDDRLIWAKPTSG
                     SDHIQLITAKGQAIRFKETDVRDMGRNATGVYGIRLHKDDAVVGMGVAKTDKDKKRHY
                     QILIIMAKGYGKRTDLSLYKVQRRGGSGIKTAKVTDKTGDITNAFLVNADVMENRDLI
                     IISNNGQVIRTPFKTVSTLGRDTQGVRIMRFKDDEDRVSGVTWI"
     gene            11537..12658
                     /locus_tag="PKK65_01380"
     CDS             11537..12658
                     /locus_tag="PKK65_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNU81366.1"
                     /translation="MDNTILISIFALILAGLIFVILLLLKKKTPINDAQSLALLERLA
                     QQSEKLSQLAAQNTEMRQELDKKLSETHRASQGQFEQTTRVIQNITGQSAKLIADVTE
                     KLAKLDETNKQVINFSSQLQNLQDILKNPKQRGVLGEYYLEETLKNVLPPNSYALQYP
                     LGKNEKTGKDLIVDAVVFVKDKMVPIDAKFSLENYERLLNTSDPEARKKIEAQFKQDL
                     KNRIDETAKYVLPERGTLEFAFMFIPSEAIYYDLLVNSVGTVKINTRDLIEYAFRDKK
                     VIIVSPTSFLAYLQTVLQGLKAMQIEEKAKEIKANIEKLGKHMLSYDEFLRKLGGSLS
                     TTVNHYNDAYHEFKKIDKDVIKITGGESNIEPLKIERPK"
     gene            12664..13608
                     /locus_tag="PKK65_01385"
     CDS             12664..13608
                     /locus_tag="PKK65_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81367.1"
                     /translation="MAGKFEDLTELEEAILKIVVFFDMFSYPLTAYEIWRYLSVEAKF
                     AETETAAKFLLARSYLGGSQGFYYLPGQDTLVTVRKQRYHYTNRKLKIARRAVRLFRL
                     LPTVEFVALSNLIGRHNLRDGSDIDLFIISKENRIWITRFFCAGLMKLLRQRPTPQKK
                     RDKICLSFYVDSGHLDLSPMADGADDYYFHFWLAGLYPLYDAGAYHHQLMQANAWLKK
                     YLPNGDFVVNNQSYRESPRYWLKTRLVLKFWQKFCDYLESVARRFQLRIMPAALKDKA
                     NLDSRVIIKPGILKLYLVDRRQEFTRRYQERLRRLFNV"
     gene            13601..14866
                     /locus_tag="PKK65_01390"
     CDS             13601..14866
                     /locus_tag="PKK65_01390"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNU81368.1"
                     /translation="MFNKLAENLSFIFLLASALFLPWQTKLIWLPAPSNYWEVSFFAA
                     SIFVFFFIIFFILSGHLRRADLKDVLEKESSLWLAVLVLLFAAFVSAGFSPLPTLSLF
                     RFFLLFLAFLFFYCLRLSPPSWRRYFIGLVLFSLAVQALIGLTQFFTQLSLANSWLGM
                     AVHHAGDLGAAVIETVDGRWLRAYGASDHPNIFGGLMAIGVLLSLYLSLRADISKYRP
                     LLLALSFLFLGALLVSFSRAAFLALLVGLLFMAWEYRHAWSFFRRPLIVWMAFSFLLS
                     SLFAWQYADLLWSRANLSSRLETISLNERAEFNKRAWQDFRAHPFFGVGLGVSTFFDY
                     QRSPDYGRLDTVWKYQPAHNYWLLAATEGGFIFVGAWIFIWLAVYKKSREHLFLGLFA
                     TVLFLSLFDHWLFSLPLSVFWVLSLFALI"
     gene            complement(15105..15821)
                     /gene="radC"
                     /locus_tag="PKK65_01395"
     CDS             complement(15105..15821)
                     /gene="radC"
                     /locus_tag="PKK65_01395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNU81369.1"
                     /translation="MPYRLQKNINQIGHGRAYEIKRIRDMAEGEKPREKMIKNGVGAL
                     SLAELLAVVLGVGTKREEVFSMSSRLLREYGEKAIAAQKDPIAIVKELKMPEAKACQI
                     VACFELGRRLFQKKPGGLITIRNAAQAFAYLKDMGALKKEQFRGLYLNNHYQLIHDEI
                     ISIGTIDASLVQAREVFRPALEYSASAIIIAHNHPSGVLKSSREDAEITNNLLAAGNI
                     LDIEILDHLIIGNNKYISII"
     gene            15900..17174
                     /locus_tag="PKK65_01400"
     CDS             15900..17174
                     /locus_tag="PKK65_01400"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNU81370.1"
                     /translation="MSKHRKILLSNKIPLILAPIVGAKTATALVMVKTGSKYEKKEES
                     GLSHFLEHMFFKGTDKRPDTLSLSAELDALGGEYNAFTSKEYTGYYVKTAAGKLGQAL
                     EILGDMLSNSKFDAVEIEREKGVIIEELNMYEDNPIMHVEDVLESCLYGDTPAGRDTI
                     GTKKNIRAFKRSDFLRYFRRQYGAHSIFVILSGSIRPADKAAAAKIFGIFPINTWRNK
                     IAVKEKQEQPLLKIVNKKTDQINLALALRTVPVGHSQELALKLLSVILGGSMSSRLFI
                     SLRERSGLAYYVKTTAEFYSDSGYLSTQAGVPKGQTEEAVKIILSEYRRIASEDVSTE
                     ELARAKDVVSGRLLMRLEASDEVASWYGRQAVLREHLSDPDEVEKKLRSLKVSDIRQA
                     ARMIVKNNGLNLAIIGQISSSEKKNLQAALKL"
     gene            17180..20143
                     /locus_tag="PKK65_01405"
     CDS             17180..20143
                     /locus_tag="PKK65_01405"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNU81371.1"
                     /translation="MTTYFESKPNSEQERAIKHRQGPLLVVAGAGTGKTTVLVNRLAD
                     ILLNDKLDSDRVLLLTFTEKAAQEMEERADRILPYGYLDLWINTFHGFCERILREHAL
                     DIGLPSDFRVLSATEQWMMLKQNFALFALDYYRPLGNPTKFITELLKHFSRLKDENIT
                     PDEYLEYANGLLAAGDQALSGAPGKGAEDDDLSMEAGRYRELAGAYQTYNKLLLDNSC
                     LDFGDLILYTLKLFHQRPNILKYYRRQFTYIMVDEFQDTNWAQYELVKLLAAPANNLM
                     AVGDDDQAIYRFRGASLANIMQFKDDFPEAVEIILTDNYRSRQDILDISHNFIRHNDP
                     NRLEDRLAINKSLRAQGKEALKGLPPAFYFLESQEEELSFVAERILAIYQRQEKTNAG
                     AAWSDFAILARSNDTADLFVKELSRRGIPNQFVSLRGLYYKPIILDCLAYLRLLDNYH
                     ESSALFRVLNLEPFKISHADIINLNRFARRKAWSLFETLQKVEAVRDLSPEAVPPIKF
                     LMKLIEKHSALISSSLPSLIFLKFIEESGLVKGLDIDRDQEIFSYLNQFYQKIKRFEE
                     NAPETRLPDFMAAMELELESGESGALRLDFDDADTVKVMTVHAAKGLEFPYVFVVNLA
                     DKKFPTINRNDKIALPEAIIKEPLTDSKDAHIEEERRLFYVAMTRAKQELYLSSAKDY
                     GGVRAKKVSQFIEEAGLVPIPVLRGGNKNELWRDLSLSGKTSISTELKPENLPKRFSF
                     SQLAAYKTCPLQYKFAFILKVPAPADKPSLIFGRVMHNVFYDFLKPLSNGPQVSLFGE
                     TNIDPDILSLKRLEKILADYWLEDGYESAAQREEYKKKAKQAIRAFYNVFCDATVPSP
                     LFLEKSFAFKIGGETLKGTIDRVDKLDGGVEIIDYKTGSPKNKLDWDSKRQLILYHLF
                     LEEVLQIKVLSLRYYYVEDGSSLSFLPKEAEKEKLREEIKLQIEAIKARKFPPQPSEM
                     CKHCDFKNICEFCQR"
     gene            20164..20841
                     /locus_tag="PKK65_01410"
     CDS             20164..20841
                     /locus_tag="PKK65_01410"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HNU81372.1"
                     /translation="MIRIGKSFHWRWLLYFLIFGVWSLWLVQQRVNSFGNYIVDDCLH
                     DIKGQRPVVLVLGAAVYENNQVSPVFADRLETAFELYQAGLVRKILVSGDHGHPLYDE
                     VNAGKDYLLAKGVLPADIFLDHAGLDTYDSIYRAKKLFQVQSFLISTQDFHLYRSLYI
                     AKRLNLDAYGCRADKRFYQNIKQMEQREWLAKIKAWLDINLGSEPRFSGDIFDLEGDG
                     RQTWDKL"
     gene            20853..21866
                     /gene="ruvB"
                     /locus_tag="PKK65_01415"
     CDS             20853..21866
                     /gene="ruvB"
                     /locus_tag="PKK65_01415"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNU81373.1"
                     /translation="MSEERLLNPQETTEEAVLDKSLRPRFLREYIGQERIKANLEIAL
                     SAAKQRKEPVEHILLYGAPGLGKTTLAHVIANEMGANIRVTSGPAIEKSGDLAAILSN
                     LEEGDILFIDEIHRLPKTVEEILYPAMEDYALDIIMGKGPSARTLRLDLPRFTIIGAT
                     TLASLLSAPLRDRFGLSYHLDFYLPTEMEKIIVRSAHILAIDLPPSAATAIAGRSRRT
                     PRIANRLLKRVRDYCQVKNQGEISLEACDQAFALLAVDYLGLDAVDRKILQVLIDKFK
                     GGPVGLNTLAAATGEEADTLENIYEPYLLQLGFLNRSPRGRLATAAAYTHLGRNLSDG
                     GLL"
     gene            21878..22927
                     /gene="queA"
                     /locus_tag="PKK65_01420"
     CDS             21878..22927
                     /gene="queA"
                     /locus_tag="PKK65_01420"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNU81374.1"
                     /translation="MSLEDFNYNLPKHLIAQTPIKPRDHARLLCLGRFNGNIRHRRFD
                     DLKDELQPGDVLVINDSKVFPARLMGRKDGSGGQVEIFLHKFIKDGRWECLVGGRVHP
                     NLKIIFSHGLWAVLEKDQGDGTWLVFFSKSGQEFWHLLDQIGQVPLPPYIQGSGRQKL
                     DRLRYQTVYAQDNHKGSVAAPTAGLHFTNRMLAKLRQQGVNIVPVTLHVGLGTFAAVK
                     ETDITKHQMHSEFMSISAEATKVLVAAKKQKRRIVAVGTTSCRVLESYGQEISRGRLS
                     LGETYQGWTNIFIYPGYQFILTDALITNFHLPKSSLLMLVSAFAGQKAVMEAYRQAIQ
                     ENYRFYSYGDAMFIG"
     gene            23045..23617
                     /gene="lepB"
                     /locus_tag="PKK65_01425"
     CDS             23045..23617
                     /gene="lepB"
                     /locus_tag="PKK65_01425"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNU81375.1"
                     /translation="MFKKILILFFEAVKVVAISLAIIIPVRYFLIQPFYVKGMSMESS
                     FQDHEYLIIDEISYRFRPIARGEVIVFRYPLNPQEYFIKRVIGLPGESVQIKDSQVII
                     YNDQYPGGLVLSEPYLDKGTPTYSNSDDKISLGADEYFVLGDNRTASKDSRSFGAVNR
                     SFITGRVFLRGWPFSRIAIFNQPVDYSDSK"
     gene            23632..24987
                     /gene="hisS"
                     /locus_tag="PKK65_01430"
     CDS             23632..24987
                     /gene="hisS"
                     /locus_tag="PKK65_01430"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNU81376.1"
                     /translation="MSRQKVNAKNMPIFKKALERRSAKAIPRLLGMKDIVPSEFRYFS
                     AVSNKAAALAELYSFNAIKTPIIESADLYKKSTRHNNKELYTLDCEKGENGALRPELT
                     QGILRAYIENNFSELPQPVRLYSIGSVFRKEKMQTGRYRESTQFNLEVIGEDKPMAEA
                     LLITAAYNFFQDLQASFQIQINSLGKADCRKEYISKLNSFYKERGRRSKLCNNCKKYL
                     GNNMMALLDCKEEACQNLLHEAPQIADCLSDESRNHFAKVLEYLDELGIPYNFNPYLV
                     RGLSYYNDTVFEFWPLNDEGVASSKLALAGGGRYDTLVESLGGRPTPSVGLAIGLERL
                     VAKVKDRLTLPNFKEEVIFLAQLGDQAKIKSLILFEELRRAGFNVRQSFSTDSLKNQM
                     EEAVRLNAKISLILGKKEVMDETIMLRDMESGVQETVAFKKIKDRLEKKVNRKEGVIY
                     G"
     gene            24980..25210
                     /locus_tag="PKK65_01435"
     CDS             24980..25210
                     /locus_tag="PKK65_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81377.1"
                     /translation="MADFKRKPGESFESFLRKFKKGLKNSKRLEKARSKQHLEPKKTK
                     RQQKKYALISIKTQRKKEYLRKIGKLEETQNR"
     gene            complement(25331..26794)
                     /locus_tag="PKK65_01440"
     CDS             complement(25331..26794)
                     /locus_tag="PKK65_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HNU81378.1"
                     /translation="MTIDRIIKKFEENHPNEDTTKLRLAYDFALEAHAGQLRKTGEPY
                     IQHPLHTAFLLTEIKADLPTIIAGLIHDVPEDTDKTIEDVRKNFGEEVATLVEGVTKL
                     GKIKYRGIERYRENLKKMFLAMATDIRVIFIKFCDRLHNLRTLEGLPEAKQKRIAQET
                     LEIYAPIAGMLGIWRLKWQLEDLCFKYLYPEKFQELEQKYEVEKKAERNQYFQKIKKE
                     LLPRLAETNLKYDLEVRFKHLYSIYQKMQRKDRKFDEIYDVFALRIIVPTIDDCYKVL
                     GIIHTIWRPKPNRFKDYIAVPKPNGYRSLHTTIFGPEGKAVEFQIRTQEMHEESLYGL
                     AAHWYYKAKNQNELQPHWVKEIVDIQKEISDTSELIKNIKLNIFNDRIFVFSPKGDVF
                     ELPEYSTPIDFAYAVHTNIGNQATGAIINDRLAKLDQELKNGDLVEIITDKGRQYPNR
                     DWLKFVKTKRARDCIKQFAKKSPIENFKRFLPSRKKE"
     gene            26885..29566
                     /locus_tag="PKK65_01445"
     CDS             26885..29566
                     /locus_tag="PKK65_01445"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNU81379.1"
                     /translation="MRRRFFSFLLFFALISAGLFSVNFVFAQNLDVGINEINNAIVLS
                     DTDPRIIIARIINIALLFLGVIAVGLIIYAGFLWMTSGGNEDKIDKAKNTLKNAIIGL
                     VIILSAWGIVSFIMSRLLGATGNGGFFNGDSGSNSALVGTGAIGACTVERVYPEDGQT
                     DVARNTSIIITFKEPILLTSVCQNAAGEACACDQASCNLINPTHIRIFRQDFGDNCGD
                     TSCPNDNTNVNQAHVSVSSDRQTLIITPHDFLGSPDGDTDYQVKFTNGILKDSGDSIF
                     KTCNTDWLQWGFRVSNNLDLTPPQVLRGGIFPLPDNEKDFYSQTVAAVAAQAAVTVNN
                     CPQVYQAPSILGVIPIAGNQNGSAILDDNFHQNVSALTVVTTPDNNQAQLFAGQELLG
                     VANWNGNQIVFSGFFQLDVEGHEAGNAWQINIQPEILADQLTVGGEIYTFTFSENNND
                     HNISISGCSGLNIIALNIFVKLSGHPDVNVDLEGNKVILIAKVAGAAGNNINLSTTNS
                     DALSLQAFSGGTDLVRVSEVKDRHDRPMNSVIQVNFNEAVNPMFVSGSADEVADYIRV
                     VNAEATATDGASCSVDADCASYKCSDNVCVGHYLSGNFLISNAYKSVEFISDVECGQN
                     SCGEKIYCLPADSHLAVELVAANLKTCNTDADCANFQPFSHCAPFFNYLTCQDVNGKN
                     YPVANLDQLDGIVDAAVNSLDGNRDVFADGPITFYNENEPANLELKDKYRWSFYINDQ
                     IMSAPPQISFIKPDNNSLKADTKAPVQINFNTLMMNASLRTGSVWINNGQKNVEHHLI
                     NLFTSSPSPVGYWVTAENRDVFPLDGEPDLTFVFLKHTELSPSVTYKSQVGSGVKDIY
                     QNCFKPSAGPNCAADANNPSCCYGVPTSLLGDDGNCQ"
     gene            29641..>30950
                     /locus_tag="PKK65_01450"
     CDS             29641..>30950
                     /locus_tag="PKK65_01450"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81380.1"
                     /translation="MLNKSSFVNKNKKFIIRIAVFLFLFGVSLTFSGLANAQIDVGVN
                     EINNDIVLSSGDPRTIAARVINIVMLVLGVLAVGLIIYGGFIWMTSNGAEDKIEKAKK
                     ILKNSVIGLVIILSAWGIASFILSRLTGATNGNGSSGSSCNSGQLLSCGCGGSMSCID
                     GSWGPCLGSDCNNGGGPSSCDSSPLSGCQAADQICSVDSFCDVDICSCLPKGELGDYC
                     DADTNTPTCEADNNKCAEYLSCNPDSCTCYGPPVITNISPAGGFCNENVDLSCTTDAD
                     CSLGCNLNTPNGTADNIITISGSNFGQYDPLVSKVIFSTDAVGLNPTALNPDCIDSWT
                     NNQIIVVVPNTATTGAVRVVTADNQSDSTNDDNGPIIEDFVVNSIVRPGVCLLTPDSG
                     LLSEKVNYRGVNLYGGEAYFGSYDKNVRGLASDFINPTGLTGTTT"
BASE COUNT         8645 a         5605 c         6985 g         9715 t
ORIGIN      
        1 tacggcgtgg actactgggg tatctaatcc cattcgctcc ccacgctttc gtccactgag
       61 tgtcagaacc gtcctagcaa actgccttcg catttggtgt tcttgctgat attaatggat
      121 ttaaccccta caccagcaat tccgtttgcc tcttccggtc tctagtttgt ccatctctcc
      181 cgctggcccg aggttgagcc tcgggattta acaggagatg cgataaacca cctacggacg
      241 ctttacgccc aataaatccg gataacgctt ggggccctcg tattaccgct gctgctggca
      301 cgaggttagc agccccttat tcatatgcta ccgtcattgt ttcgtcacat ataaaagcac
      361 tttacacccc gaaaggcttc ttcgtgcacg cggtgtcgct ccttcaggct ttcgcccatt
      421 gaggaagatt cttgactgca gcctcccgta ggagtctggg cagttctcag tcccagtgag
      481 gcgggtcacg ctctcgcgcc cgctacccgt catcgccttg gtaagccatt accttaccaa
      541 caagctgata ggacataggc ccctctcgaa gcgtcggagc tttaatggca gaacttatgt
      601 tctgacatac tatcgggtat tatcctcact ttcgtaaggt tatccccgtc ttcgaggtag
      661 gttaccaatg tgttactctc ccgtttgcca tgcccatatt gctatgagca ttcgacttgc
      721 atgccttaga cacaccgcca gcgttcatcc tgagccagga tcaaactctc tgtttgaata
      781 tagagatatc attttcaaaa aaatgatatt aaagattctg gttgatttat tatccgaatt
      841 gatgccgatt cgcatcaaaa tttttgttta tttcaccaag ttaactttgt taaagttcgt
      901 cgaatgaaac atttttagat aggacagaaa aaaacagaga tatcttaaat accttgtttt
      961 aactaagctg ttcttttttt atgtttcaag tatcaccatt ttatatcatt tgcgcggggc
     1021 tgtcaagagt ctctttggtc cccattttta ctaggaaaag acttagtttt atgctattat
     1081 ttgtttaaga gattttttgt taaaaaattt attttgtatt tttatggaag actctaaaca
     1141 gaaggctatt gccgaaattg aagatatatt tagcagagct aaagaatctt tattatcctt
     1201 acataatcag aagatggcgc ttattaaaaa attcaaagac gctgaagacg ctgctcaagc
     1261 ggcggatatt ttagataagc tcaaacagct taattagaat ttattttatc aatatgtcca
     1321 cagaaaaatt aaaagtaaaa gaggaagaat ctttagcctc attattacca gacttggcaa
     1381 aaatcaagca gtggcaaaaa gatattaatg ccgttaaagg tgataagatt aaaacagcgg
     1441 aactgagaag gaaatttaag aatatttttg gcgtaccttt tggtcaaaaa ttagttgccg
     1501 accttattcg cttagaaaag ggcgagggta aggaaaaaga aaaagaagaa aataaagaga
     1561 aaccggtcca gcccttagtg gcggtagttg agggggcaaa aacattacag gcagatgtcc
     1621 acgagcagca gtccttagag gtggccatgg cgcaaacaat aattaatcca gaattaaata
     1681 agcaaacttt atatattgat gagaaaaaaa aggaagttgt cgctaacgct atcgatgggg
     1741 cggaggatgt cgctttaaaa gcttccaaag aggccgaaga aggagaaact actgttgata
     1801 atcaaactaa tggcgaatta aaaaaagaag atgagatgga tagagttgat gaagaggcct
     1861 tacgacagga gatattatct acgaaggaag cggttttagc ggaaattaat gctggttatc
     1921 aggcggaacg gaaagcccat aattttgaat tagaggaaaa aattaggcaa gccgattcat
     1981 ttgattcttt attaaacata attaaagaat cggaaggcat acaaggatca aaagaatttt
     2041 ttagtccaga gatgttgttt aatgtgctga cgtctatacg ttcaggggag gcgcctttat
     2101 cagcggctac ctcgggatta ggttttagag aaacagttga acggctttta tcatccgaac
     2161 ataaaatcat ggaagccgag gtgtcaaagt gcaattcttt agaagacttt atttctcttt
     2221 taaataaatt tcccgaatta gccgttttgg gcggagatat aaaaaatatt aatgaatata
     2281 tatcttattt ttccagagaa gttgaattta cggagcagct ttatatagct gataatttta
     2341 aaaacgaaga acatcttcac aacgctttaa attttgccac tcgtcgctac ggcctaagag
     2401 ataaggttac acaatatatt attaagaaga acggccttca ttttaaagac gatcctgcta
     2461 aaaaaatcag ggaagaagtt aatgatcccg tcaataatgg tttagaacat gtagacggcc
     2521 ccaaagaaaa caaatctgaa agccaaaaaa gagagaaaca aattgcccca gaggccttag
     2581 attattttta tgaccttggt ttgaatgata aagatttact ttccattcct ggcttgaccg
     2641 aattaaatag tgtttctcaa ttattaattg ctaagagttt gaaaaattta gcccttgaga
     2701 aggctaatag gaatgtcagc aagaagcagg cggatgccag cgccgttgct aagggttttt
     2761 ggggtaaatt aagcacgggc atttctaatg cttttaaaat tggtaaacaa aagaaggagg
     2821 ctattttaga acaagcgcat ggcggcttgg ctgaacataa agttgaatta acgcgtttgg
     2881 tcgattgggc gcaaaacctt gacttgaaag aaattgagac cgaagaagga agatttatgg
     2941 ctgattttac cggtcatttc gatttgaaga atattaatga aaagcaggcg gcgattgtca
     3001 aagaaatgaa tgagaatgct aattggttgg cctcttgtgc caaagagtac cgtctcttta
     3061 cttatactcc ggagcctaag ggttcaaaga cgcatcagga atattatcaa gctaaacggg
     3121 cctatgatga ttcccgcaaa cagatgagcg ccttgttgtc taatgattta aactggtcgg
     3181 aagcttcagt tttacaagct attaataaaa tggatgctaa tgtaaacatg gtgcagttta
     3241 tgtcggctaa tcctgattta gaaaaagatt ggcaggagat gattaaaaat aaatcagttc
     3301 tggccaaagc ttttgctaag gataattgga aattttttgc cagtggttat gccggccgaa
     3361 tggcccttgg tagtgtttta ggatttgttg ccattccggc tgtcgcaatg gctgtaggcg
     3421 cctggcgtgg ccgggaaaag gggcggcaag cattgcgtaa cgccgataag ggtttagaaa
     3481 aaaaagattt gcctggagat agccctttac ttacagaaag aaaaatggtt ttgaaggcga
     3541 ttcaggaatt agtgccgact gaatatgctt tcaatagaga agaatggttg gctaaggtcg
     3601 ctaatccgga gcagaaatct ttgtattatg atttaagggt taagtttaat gatcttgatg
     3661 cgcgttggcg tcaggaggaa gaaaaaaaga agggggaaca caataaagag agaaatcaag
     3721 aatatacatc tggaaaaaat attgagcgca aagtgttacg cgcagagact ttaatgtcta
     3781 agttgcagaa tttgattgat aaagcgcagc aggccgaagg tgaaaaaaga gcgcaacttt
     3841 tatttcaact ttcccggcgg gttaattttt ctaaagattt agttgataag ggattaatta
     3901 attttggttc ggttgaagaa agaagttccc gcatgctcga cttctatcaa ttattatctc
     3961 aaggacaaat tttacaatta gataaggatg tgcaggtggc gctcgtagcc aaaggagatt
     4021 atgaaagtgt ttatgaagac gtcaacatta aagaattaag caaattaaac gagcgggctg
     4081 aggatttatt aaattctttg gagtattttg cggagatgca attagataag aataggcggt
     4141 ctttcttaaa gaaaaagatt ttgcaagggg ctattgtcgg cggcgttttt gccacttctg
     4201 gcgctttagt ccgcgaatta tccggttctt ggcttgaaga caaagccgct gctggtttcc
     4261 gttttatttc tgaaaatact aaattcggag cagccgtctc tgatactatc tctgatatta
     4321 aaaattacat cagccctaat cttaatagtt tagctggttc aagcgctgtt gagacggcca
     4381 cttggtctga tcaaattagc aatgagggct taaaggctgg acaatatgat tctgtttggt
     4441 tgtcgactaa aaatattttt gaaagccatg ctgccgaact cggctatcaa ggcgatatta
     4501 atgatgtcgc tgctttacgc cattgggcag aaatacagac taatcgcact ttagctgata
     4561 ctggcgatat cactgataaa gtttttgtcg gcaataaagt gacgctagaa aaaaatggag
     4621 ataactttat tgtgagagtg gaagctggca gcggcgctca gccgggatat ttatcagaaa
     4681 caagcattga tttgccatct ggaaaagatg gcttgcaaga aacgccgcct aatccggatg
     4741 gtagccagcc gatacaaacg cctgacacct ttattgattc caaaggagtt cccaatgtcg
     4801 gcaaggttag agaaatttgg gcggatagag ctggcgcccg tttcggtcta tcaagcgaac
     4861 aagtttccta tgccgacgag aacgttatta aggcagaaat tgacggctac gatgtcttta
     4921 ttgatactca gaataatact tttctatttt ttgacgataa cgccaatgaa gtatccggct
     4981 tgttagtgga tgataatggt catctaataa ataatgctga agaattttta aggagtaaat
     5041 ttttctcaga ggaggcgtcg gcggcagggg caatcgatta tttatctaat gataatgttg
     5101 aacaggatgc gtcctcgtct ttacccgaat tagagccttc tcttccgtcg tcacagacag
     5161 aagcactaca atcgcttaac cagtcggaca tccccaatca gtttgataat atcattaata
     5221 attatgttcg agagactact ggcggcagta ttaaagaagc tttagagatt attgctgctc
     5281 gaactaaaac tccagccgat aaagtgttgt tagaatatat tgaagaaaat catatttctc
     5341 ccgatgcttc tttaagtgat ttactctcca gtgatgccgg tttattaatg tcgatgaatt
     5401 ccccagaaaa tagtgtctta acggaactat atgatttgcg ccacgcttct tccagccagg
     5461 ctcaagccga aatgttatat aaatatttac acaccgcctt atctgaagat ttaaaggatg
     5521 cgggcaagtt ggcacacttt aataagcttg cttccttaaa tgcttttcgg gttggttcaa
     5581 gtttggatgg caaagttgac actttggtga tgcaggtagg cgataatgtc cggaaagtcg
     5641 atttctcggc taagggtata agcgaagtgc ttaaatttgt gaaacagatg ctttaaggta
     5701 aagttagtat aataaaaaca ggctagtatt agtctgtttt tgttatatta gagatgaatt
     5761 ataaaatcag ccaattttca gcccatttta tattgacata ttttataaaa tatgctacct
     5821 ttaatagaat ccccaaaaat tagggaagtt aattaaaata aaataaccga taaaaaccaa
     5881 taaataaaat gaaaatgaaa aagatgtttc cagtagctat tttagccact ttttttgtgg
     5941 ccttttccat taaggctcag ccgaccgtaa aaaacccatc aaacgggatg tcggtggagc
     6001 tctcttataa tgggcggcag atgattttgc ggccggccga aagtaaggca atttattttt
     6061 tgcctccctc tggcgaggct cgcaatgtgc gtctccgcta tttcgtgggg gaagatataa
     6121 aaaccgtttt taccactaac ttaaacatag tgagcggtca agaattggtt ttaccgcttt
     6181 ccgcccccga gaggatacgt gcgactgttg acaagcagtc tgcttctcag aaagatcaat
     6241 caacaaccgc tagcactatg ccagggtttt cgcccaaaat tccagaggtt tctggagttt
     6301 tagaggtgaa gaaatttccc ttaactctaa ttgactcaac ccaaaagtca attttagttt
     6361 ttgagggaga cttctacggc gctgccttgg cgccaaatga acgaactgag ccgattatta
     6421 ccggtcctgg catcatttca atgaaaattc tgtatgatgc cgatcccccg gcatctagta
     6481 ccggtaagaa tctctggcaa tcccctgttc agggtatcgt tacccagggg caggaatatt
     6541 ttgttttaag agagagtcac cttcgtaatt tacagcggga tgtggccaag gttgtgtttt
     6601 tcaacccgac accatattct atggtgcctg atggtttaga cattgatcct attccgccga
     6661 accgaagaag taaggcggta aggttgaacc agggttttaa taacttcagc ttcgtttata
     6721 ttaacgaagc gggagttaag gtgagggccg tattcgaact agtcgtaact aagggaaccc
     6781 cagtggttcg tttaaacctt aaccctctcg gcaatgccta cggggttaca ggcaattaaa
     6841 atatttgttt tgtacattta ttccggcttt cttcttagag agccggtttt tttattgatt
     6901 tatgcccaat ttttaggctt aaattgacct gaggctttgg ctctgatata ataaaaataa
     6961 gctgagaggg cctattttta ttgtatttat caataaatta tttttatttt agcctctaaa
     7021 attgttcgcg attcttattt ttaagcacta tttatatgat tcttacttgg ctcggacatt
     7081 cttgttttaa actgcaagac aaaatcggtt ctggcggcgt caccgtggtt accgatcctt
     7141 tcggtaaaca gtttggctta aaattaccta actgtgaagc cgatatcgtg actatttccc
     7201 ataatcatga agaccataat aacgtggccg ctttgcgcgg taatcctttt gtgattaatt
     7261 gcgctggcga atatgacatt aagaatgtct tgattgaggg cattgattct tttcatgacg
     7321 acgttcaggg tgcggagcgc ggcaataatt taatttttcg tttggaaatt gatgatattt
     7381 ctgtggtcca tctgggcgat ttaggccagc ctttagatag cggacagtta gagaaacttg
     7441 ccggaacgga tattttgttg attccggtgg gtggcaaata tactttagac gcgaagaaag
     7501 cggtggaagt gattgcccag attgaaccgc ggattattgt gccgatgcat tatcaaaccg
     7561 atgaattaaa gatagagggt ttggacgggg ttgataaatt tatcaaagaa atcgggttat
     7621 ctccttctta tgaagaaaaa ttaaaaatca gcaagaaaga tttgccggtg gaagacatgg
     7681 agcttgtgat actaaaatat taattccaaa tttttataat tcttaattat taattcctat
     7741 gattactcat cctaagaaag aaagaacttt ggttattctg aaaccggatg ccatccagcg
     7801 cggtttaatt ggtgaatcta ttaagcgttt tgaagccgcc ggcttgaaac ttgtagcttt
     7861 gaagctgata atggcacaag aagaagtttt gtggaagcat tataataaag acgatgcttg
     7921 gttccagaaa aaaggcgaat tgaccgttaa gaatcgccaa gatttaggta tgccgataga
     7981 gaaagagccg attgaatatg gcaaagatat tattcgcgct ctggtaaaat ttatgagctg
     8041 cgggccaatt gcgccgatga tttgggaggg caatcaggcg gtggcggttg tcaaaaaaat
     8101 cgtcggcggt actgagccga ccacttccga tgtcggcacc attcgtggcg acctgactct
     8161 tgattcctat gagctttcca atcgtgatga aagggcggtg cgtaatttag tgcattgctc
     8221 cgatgaagtg tctgaggcag tgagagaaat taacctttgg tttaaaccgg aagaaatttt
     8281 agaatatcac ctaattgccg aacagatttt atatgatgtt aatttagacg gcattaagga
     8341 ataattaaat atatgtctaa gcaggaaaaa aataatcaag tggccgagga aaaaaagaag
     8401 cgttttttct taaaggtggg cgtctgggtt ttttccgtgc ttattcttag tttttggctc
     8461 tttactttgg ttcattcttt taataatact actattagtg tggggcagac tattgatgct
     8521 gacaccgccg cttggcaagc tgaccttaac caaactattg atattattag aaatgagatg
     8581 gtagataaaa cctcggtagc ttctaatacg cccgcagtca gtcaggattt tttagagaaa
     8641 atgaatgcta atttagaaga gcaggtagct gcttcagaaa ctgcgccgat agtggaagaa
     8701 aatattacag acaaatcaag ggctactatc accccggaag agcttatctc cggtctgcaa
     8761 aagcgtcttc cggaattagc ttcatcttct tgcccggaat ttatcgattg tatgcccagt
     8821 attggcgagg ctaaaccttg cgtgattccg ccgggctgtg aaaatattac gcaaattgca
     8881 tattgatatg aaaaaagaaa acaaagatgt aaaaattgga aaagaggaaa gtaataacaa
     8941 taccatttta cctaacggac ggcgccagac cgatttcggt ttaatccagg accaaccttt
     9001 ggttgaggaa atgagccgct cttatctcga gtacgcgatg agtgttattg tctctcgagc
     9061 tttgccagac gtacgcgacg gtttgaagcc ggtccatcgc cgcattttgt atgcgatgtg
     9121 gaatatcggt ttaagggcaa atggaaaatt tagaaaatca gccacagtag tcggtgaagt
     9181 tttgggtaaa tatcatcccc atggcgacag cgctgtttat gaatcaatgg tgcgcctggc
     9241 tcaggatttt gcaatgcgct atcctttggt cagggggcaa ggaaatttcg gttccatgga
     9301 cggcgatagc gccgccgcta tgcgttacac cgaagctaaa ttggccggca ttgccgagga
     9361 aatgttgttt gatattgata aagatacagt tgactttatt cctaattatg acggttctca
     9421 aaaagagccg cgtgtgctgc cggcgaaatt acccgctctt ttattaaacg gtacgatggg
     9481 tattgccgtt ggcatgtcca ctaatattcc gccgcacaat cttggcgaat taacgcaggc
     9541 gatttgtcat ttgcttgata accccgaagc gagtattgag gacttgatgc aatttatcaa
     9601 aggtcctgat tttcctaccg gcggtattat ttataatcaa aaagatattt tggcggctta
     9661 cgccaccggt aaaggcagta ttgttatccg cggcaaagcg gaaatagaag aacagaaagg
     9721 cggcagtttt caaattgtaa tttcggaaat accttatcaa gttaataaag ctgatttagt
     9781 ggaaaagatt gccgatttag tgaaagagaa aaagcttgac ggcattaagg ccttgcgcga
     9841 cgaatcggat aaggacggcg tccgtattgt ggcggaatta aagaaggatg cctatcccaa
     9901 gaaaatcctg aatgctctat acaaacacac tgctttgcag actagttttc atgtgaacat
     9961 gctggcttta gttgatggca tccagcccaa agttttaact ttgaagatga ttttagaaga
    10021 gtatatcaaa catcgggaag aagttgtgcg tcgtcgcact caatttgaat tgaataaagc
    10081 caaagaccgt tcgcacattt tggaaggttt actgatagct ttaaaaaata tagacgaggt
    10141 gattaagacg attaaggcat ctaaagataa agaagtggcg aaagctaatt tgattaagaa
    10201 gtttaaatta tctgagcgtc aggctattgc tattttggaa atgaaactgc agagtttggc
    10261 caatcttgaa cgtttgaaaa ttgagcagga aggaaaagaa aagcagaaac ttattaaaga
    10321 attggaaagt attttgaagt ccgtggcgaa gattcggggc atcattaaag aggagattat
    10381 ggctgtggcc gataaatttg ataatcctcg ccggaccgct gtcgtggttc atggcgtcaa
    10441 ggaatttagt ttggaagatt tagtgccgaa tgaagaaaca atggtcatga tgacgcgcga
    10501 cggctatatc aagcgtttgg agccggatac ttttaaggta caggctcgtg gcggcaaggg
    10561 tgttatgggt ttaactacta aggaggaaga tacggttgaa tttatgttta ccacttttac
    10621 tcacagcgat attttattct ttactactcg cggtcgggct ttccagttga aggcgtatga
    10681 aattcctcaa gcttctcggg ttgccaaggg taattcaatc gttaattttt tacagttgac
    10741 cagcggcgaa caagtaactt ccgtgctgcc gcttgataag attttcggta gtaaatattt
    10801 attctttgcc accgagaagg ggttggtaaa gaaagttgaa ttatccgctt ttaataatgt
    10861 ccgtcgcagc ggtctgattg ccattaaaat taaggaagat gatcgtttga tttgggcaaa
    10921 gccgacttcc ggcagcgatc atattcagct tattaccgct aaagggcaag caattcgttt
    10981 taaggaaact gacgttcgtg atatgggccg taatgctacc ggtgtttatg gaatcagatt
    11041 acataaagat gacgctgtcg tcggcatggg agtggctaaa accgacaaag acaagaagcg
    11101 ccattatcag attctgatta ttatggctaa gggttatggg aagcgtaccg atttatcctt
    11161 atataaagtt cagagacgcg gtggtagtgg tattaagaca gcgaaagtga ctgataagac
    11221 tggtgatatt accaatgctt tcttagttaa tgccgatgtg atggagaacc gtgatttaat
    11281 tattatttcc aataatggcc aagtaattag aactcctttt aagacagtaa gcactcttgg
    11341 tcgtgatacc caaggggtgc gtattatgcg ctttaaggac gatgaggata gggtatcggg
    11401 tgttacttgg atttaattac tgtttacgtg gcaatactct tttaatttta agtagagaga
    11461 attatcccct taaaaagctg tctttataag acagtttttt gtgaaaaaca taaaactggg
    11521 ctataattat aaacatatgg ataacactat cttaatttca atttttgctt taattttagc
    11581 tggattaatc ttcgtaattt tacttttatt gaagaaaaaa acgccaatta acgatgctca
    11641 gtcgctggcg ctgcttgagc gtttggccca gcaatcagaa aaattgtcgc aattagccgc
    11701 tcagaacaca gaaatgcgcc aagaattaga taaaaagctt agtgaaactc atcgcgccag
    11761 tcagggacaa tttgagcaga caaccagggt aatccaaaat attaccgggc aatcggctaa
    11821 attaattgcc gatgttaccg aaaaactagc caagttggat gagactaata aacaagtaat
    11881 taatttttcc tcacagttgc aaaatttaca agatattttg aagaatccga aacagcgggg
    11941 tgtcttgggt gagtattatt tagaagaaac tttaaaaaat gttttgccgc cgaactctta
    12001 tgcactgcaa tatcctttgg gaaagaatga gaagactggt aaagatttaa ttgtggacgc
    12061 ggtcgtcttt gtcaaagata agatggttcc tattgatgct aagttttctt tggaaaatta
    12121 cgaacgcctg cttaacacta gtgacccaga agcgcgcaag aaaattgagg cgcaattcaa
    12181 gcaggattta aagaatcgta ttgatgagac cgctaaatat gttttacctg aacgcggcac
    12241 tctggaattt gcttttatgt ttattccttc ggaagcgata tattatgatc tcttagttaa
    12301 ttccgtgggc acggtcaaga ttaatactcg cgacttaatt gaatacgctt tccgcgacaa
    12361 aaaggttatt attgtctccc ccacttcttt tttggcttat ttacaaacag ttttgcaagg
    12421 cttgaaggcg atgcagattg aggagaaagc gaaagagata aaagctaata ttgaaaagct
    12481 gggtaaacat atgctcagct atgatgaatt cctacgcaag cttggtggca gcctgtcgac
    12541 caccgtcaac cattataatg acgcttatca tgagtttaaa aagattgata aggatgtgat
    12601 taaaattact ggaggagaaa gcaatattga gcctttaaag attgagcgcc ctaaataaaa
    12661 tctatggccg gcaagtttga ggatttaaca gaactagaag aagcgatttt gaagattgtt
    12721 gtgttttttg acatgttttc ttatccctta accgcttatg aaatttggcg ttatttgagt
    12781 gtagaggcta agtttgctga gactgaaaca gcggctaaat ttttactggc gcgttcttat
    12841 ttgggcggaa gccaaggttt ttactattta ccggggcagg ataccctggt tactgtgcgt
    12901 aaacaacgtt accattatac taatcgtaag ttaaaaatag cccgtcgagc cgtacgttta
    12961 tttcgtttat taccgaccgt tgaatttgtc gctttaagta atcttatcgg tcgtcataat
    13021 ttgcgtgatg gcagtgatat cgatcttttt attatttcca aagaaaatcg tatttggatt
    13081 acacgttttt tttgtgccgg tttgatgaag ctgttgcgcc aacggccgac gccccaaaaa
    13141 aaacgcgata agatttgtct tagcttctat gtggatagcg gacatttgga tttaagtccc
    13201 atggctgacg gtgctgatga ttattatttt catttctggc tggccggtct ttatccttta
    13261 tatgatgctg gcgcctatca ccaccaatta atgcaagcta acgcttggtt aaaaaagtat
    13321 ctgcctaacg gcgattttgt cgtcaataat caatcttatc gcgagtctcc ccgttattgg
    13381 ctaaaaactc gtttagtttt aaagttttgg cagaaatttt gcgattattt ggagagtgtc
    13441 gccagacgtt ttcagttgag aataatgcca gccgctctta aggataaagc taatttggat
    13501 tctagagtga ttatcaagcc gggaatctta aaactatatt tagtagatag gcgccaagaa
    13561 tttactcggc gctatcaaga gcgcttgcgc cgtttattta atgtttaata aattggcaga
    13621 aaatttaagt tttatttttt tactggccag cgctttgttt ttgccttggc agacaaaatt
    13681 aatttggctg cccgccccca gtaattattg ggaagtctct ttttttgcgg cttctatttt
    13741 tgtttttttc tttattattt tttttatttt atccggccat ctgcgccgag ccgatttgaa
    13801 ggatgtttta gaaaaagaaa gttctttgtg gttggcagtt ttagttcttt tgtttgccgc
    13861 ttttgtgtct gctggttttt ctcctttgcc caccctttct ttattccgtt tctttttatt
    13921 gtttttggct tttttatttt tctattgcct gcgtctctcc cccccgtctt ggcgccgtta
    13981 ttttatcggc ctggttctgt tttctctggc cgtgcaggct ttaatcgggt tgactcagtt
    14041 ttttactcag ctttctttgg ccaactcttg gctgggtatg gccgtacatc atgccggtga
    14101 tttgggagcg gcagtgatag aaaccgttga tggacgttgg ctgagagctt atggtgccag
    14161 cgatcatcct aatattttcg gtggcttaat ggctattggc gttttacttt ctctatatct
    14221 ttctctgcgc gctgatattt ctaaatatag accattatta ctggcccttt catttctttt
    14281 tctaggcgct ctgcttgttt ctttttccag agccgctttc cttgctttat tggtaggttt
    14341 attgttcatg gcttgggaat atcgtcatgc gtggtcattt tttcgtcgcc cgctaattgt
    14401 ctggatggct ttttcttttt tgttgtcctc gctttttgct tggcaatatg ctgatttatt
    14461 atggtcgcgc gctaatctta gtagccgctt ggaaaccatt tcccttaatg aacgggcgga
    14521 gtttaataaa cgcgcttggc aagattttag ggcgcatcca ttttttggcg tcggcttagg
    14581 ggtttcaacc ttttttgatt atcagcgttc ccctgattac ggtcgactgg acactgtttg
    14641 gaaatatcag ccggctcata attattggct tttggcggcg actgagggtg gttttatctt
    14701 tgtcggtgct tggattttta tctggctggc tgtttataaa aaaagccggg agcatttatt
    14761 tctcggctta tttgcgactg tgttattttt aagcttattt gatcattggc tttttagttt
    14821 acccttgtct gttttttggg ttttaagtct atttgcctta atatgataat ttgtctgcag
    14881 gagaaggaga tcatctatta agtcgcggaa agcttttttc agtaactggc gttctttaat
    14941 aggtgttttt ttaatttccg aatctaatct tttttcaatc agagaaatca gagttgccgc
    15001 cgttccgaaa taatcattat tgtcaacgtg aaacttgacg ctgctcgcat ctttcaatag
    15061 gcgtttattt gctcgcaatt tataattgtt gatcttttta ttcattagat gatgctgatg
    15121 tatttgttat tgccgataat tagatggtca agaatttcaa tatctaaaat attaccagcc
    15181 gctaagaggt tgttggttat ttccgcgtcc tcacggctgg attttaaaac accggaggga
    15241 tggttgtggg cgataataat ggcgctggca ctatattcca aggctgggcg aaaaacttcg
    15301 cgcgcctgca ccaaagaggc gtcaatggtg ccgatggaaa taatctcatc atgaattagt
    15361 tggtagtggt tgttgagata aaggccgcgg aattgttctt tttttaaagc gcccatatct
    15421 ttaagatagg caaaagcctg agcagcgttg cgtatagtaa tcaatccacc tggttttttt
    15481 tgaaataagc gccgaccgag ttcaaagcag gcgacaattt ggcaggcctt tgcttctggc
    15541 atttttaatt ctttcacgat ggctatagga tctttctggg ccgcaatcgc tttttctcca
    15601 tattcacgca aaagccttga ggacatggaa aaaacttctt cccgtttagt gccgacgccg
    15661 agaacaaccg ctaagagttc ggctaggctg agggcgccga cgccgttttt tatcattttt
    15721 tcccgcggtt tttctccttc tgccatatcg cggatgcgct ttatttcata agcacggccg
    15781 tgtccgatct ggttaatatt tttttgtagg cggtagggca tatcattaag aaattaaaga
    15841 ttttgacttc gcttttcatt atatcagaga tgtgttaaat ttaaaatata attatttata
    15901 tgtctaaaca tcgaaaaata cttttgtcaa ataaaattcc gttaatatta gcaccaatag
    15961 ttggagcaaa gactgccacc gctttagtaa tggtcaagac cggatccaaa tatgaaaaaa
    16021 aagaagaaag cggtctatct catttcttgg agcacatgtt ttttaagggc acggataagc
    16081 gtcccgatac tttgtcttta tcggctgaat tagacgcgct cggcggtgag tataatgctt
    16141 ttacttcaaa ggaatatacc ggctattatg tgaagacggc tgccggtaaa ttaggtcagg
    16201 ctttggaaat actgggagat atgcttagta attctaagtt tgacgctgtc gagatagagc
    16261 gtgagaaggg tgttattatt gaggaattaa atatgtatga agataatccg ataatgcatg
    16321 tcgaggatgt cttagaatct tgtttatatg gcgacactcc tgctggccgg gataccattg
    16381 gcaccaagaa aaatattcgt gcttttaagc gatcagattt cttacgttat tttcgccgtc
    16441 aatatggagc gcatagtatt tttgtgattt taagtggttc cattcgtccg gccgataagg
    16501 cggcggccgc caagattttt ggcatatttc caattaatac ttggcggaat aagattgctg
    16561 taaaggaaaa acaagagcag ccgcttttaa aaatagtaaa caagaaaact gatcagatta
    16621 atttggctct ggctctgaga acagtgccgg tcggacattc tcaagaattg gccttaaaac
    16681 ttctgagtgt tattttaggc ggttcaatga gctcgcgctt atttatttct ctgcgcgaac
    16741 gttccggttt agcctattat gttaaaacaa cagccgaatt ttattccgat tctggttatt
    16801 taagtacgca ggcgggtgta cccaaagggc agacggaaga agcggtaaaa attattcttt
    16861 cagaatatcg ccggatagct tctgaagatg tttccacaga agagttagct cgggccaaag
    16921 atgttgtctc tggacgatta ttgatgcggc ttgaagctag cgatgaggtt gccagttggt
    16981 acgggcgtca agcggttttg cgtgaacact taagtgatcc ggatgaagtg gagaaaaaat
    17041 tacgttctct aaaggttagt gatattaggc aagcagcgag gatgattgta aaaaataacg
    17101 gtttgaatct ggcgattatc ggccagattt ctagctcgga aaagaagaat ttgcaagctg
    17161 ctttgaaatt ataagattta tgaccactta ttttgaatca aaacccaata gcgaacagga
    17221 acgagcgata aaacatcgcc agggtccttt attggtggta gctggcgctg gtactggtaa
    17281 gacgacagtt ttagtaaatc gtctcgccga tattttattg aatgacaaat tagatagcga
    17341 tcgggtgttg ctccttactt tcaccgagaa agcggctcaa gaaatggaag agcgcgctga
    17401 ccgtattctc ccttatggtt atctagattt atggattaac actttccatg gcttttgtga
    17461 aagaatttta agagaacacg ctttagacat tggtttgcct tcagattttc gtgttttaag
    17521 cgccaccgag caatggatga tgttaaagca aaattttgca ttatttgctt tggactatta
    17581 tcgtcctttg ggcaatccca ctaaatttat taccgaatta ttaaagcatt tttctcgttt
    17641 aaaagatgaa aatattactc ctgatgaata tttagagtat gccaacggtt tgctggcagc
    17701 cggcgatcag gctctttctg gcgctcccgg aaagggcgct gaggatgatg atcttagcat
    17761 ggaggctgga cgttatcgtg aactagcggg tgcttatcag acctataata aattgttgct
    17821 cgataatagc tgcttagatt ttggagattt gatactttat acacttaaat tattccatca
    17881 gcgtccgaac atattgaaat attaccgccg gcaattcact tatatcatgg tggatgagtt
    17941 tcaagacaca aattgggcac aatatgaatt agtaaaactt ctggctgccc ccgcgaataa
    18001 tctgatggca gtaggtgacg atgaccaggc aatttatcgt tttcgcggcg cttctttagc
    18061 taatatcatg caatttaaag atgattttcc ggaggccgta gaaattattt tgaccgacaa
    18121 ttatcgttct cgccaagata tccttgatat ttcccataat tttattcgcc ataacgaccc
    18181 taatcgcctg gaagacaggt tggcgataaa taaatctttg cgtgctcagg gcaaggaggc
    18241 cttaaaagga ttgccaccgg ctttttattt tttggaaagc caagaagagg aactgtcttt
    18301 cgtcgccgaa aggatattag ctatttatca acgtcaggaa aaaactaatg ccggcgcggc
    18361 ttggtccgat tttgccattt tggcccgttc taatgatacc gctgatttgt ttgtgaaaga
    18421 attgagccgc cgcggcattc ctaatcagtt tgtgtctttg cgcggtttgt attataagcc
    18481 gattattctc gactgcttgg cgtatttgcg tcttttggat aattatcatg agtcatccgc
    18541 tttatttagg gttttaaatt tagagccttt taaaatttct catgccgata tcattaatct
    18601 taatcgcttt gcccgccgta aggcctggtc tttattcgag actttgcaga aagttgaagc
    18661 ggttcgcgat cttagcccgg aagcggtgcc gccgattaaa tttttaatga aactaattga
    18721 aaaacattcg gcgctgatta gtagctctct gcccagctta atctttttaa aatttattga
    18781 agagtctggg ttggtaaagg gcttagatat tgaccgtgac caggaaattt tttcctatct
    18841 caatcagttt tatcagaaaa ttaagcgttt tgaggaaaat gccccggaaa ctcgtttgcc
    18901 tgattttatg gccgcgatgg aattggagct tgaatcaggt gaaagtggcg ctttgcgtct
    18961 agattttgac gatgcagaca cggtaaaagt aatgacggtt catgccgcta aaggtttaga
    19021 attcccttat gtcttcgtgg ttaatctggc ggataagaaa tttccgacca tcaatcgcaa
    19081 cgacaaaatc gctttgcccg aggcaatcat taaagaacct ttaaccgaca gcaaagatgc
    19141 tcatatagaa gaagagagaa ggctttttta tgtggctatg accagagcta agcaggaatt
    19201 atatctaagc agcgctaaag attatggtgg cgtcagagca aagaaagttt cgcaatttat
    19261 cgaggaggcc ggcttagttc cgattccagt tctacgggga gggaataaaa atgaattatg
    19321 gcgagattta tcgctgagcg gcaagacttc aatatcaacg gaattaaagc cggagaacct
    19381 gcccaaacgc ttctcttttt cccaattagc cgcttataaa acttgtcctt tgcaatataa
    19441 atttgctttt atcttgaagg taccggcgcc ggcggataaa cccagcttga tttttggccg
    19501 ggtgatgcat aatgtttttt atgatttttt aaagccttta agtaacggtc ctcaggtcag
    19561 tctttttggc gagactaata ttgaccccga tattttaagt ttaaaacgtt tggagaaaat
    19621 tttagccgat tattggttgg aagatggtta tgaaagcgcc gctcaacgcg aagaatataa
    19681 gaagaaggcc aagcaagcga ttcgcgcttt ttataatgtt ttttgcgacg ccaccgtccc
    19741 ttctcctctt tttttggaaa aaagctttgc ttttaaaata ggcggtgaaa ctttaaaggg
    19801 gacgattgat cgcgtcgata aacttgatgg cggggttgaa attattgatt ataagacggg
    19861 tagccctaag aataaattag attgggatag caagcgtcag ttgattttat atcacttgtt
    19921 tttggaggag gttttacaaa ttaaagtttt gtctctaaga tattattatg ttgaagacgg
    19981 cagttctctc tcttttcttc ccaaagaggc ggaaaaggaa aaattaagag aagaaataaa
    20041 actccaaatt gaagcgatta aggcacgaaa gtttccgccg caaccctcag aaatgtgtaa
    20101 acattgtgat tttaagaata tttgcgaatt ttgtcagaga tagtttaatt aatattcata
    20161 tctatgataa ggataggtaa atctttccac tggcgctggc tattatattt tttgattttt
    20221 ggtgtttggt cgctgtggtt agtgcaacag cgcgttaatt ctttcggtaa ttacatagtt
    20281 gacgattgct tgcatgatat taaaggacaa aggccggttg ttttagtttt aggcgccgct
    20341 gtctatgaaa ataatcaggt tagtcctgtt tttgctgacc gcctggaaac ggcttttgaa
    20401 ttatatcaag ctggcttggt gcgcaaaatt ttggtgagcg gtgaccatgg tcaccctctt
    20461 tatgacgaag tgaatgccgg taaagattat ctgttggcta agggggtttt gccagctgat
    20521 atttttttgg atcatgctgg ccttgatacc tatgatagta tttaccgagc taaaaagctt
    20581 tttcaggttc aatctttttt aatttccact caagattttc atctttaccg ttctctgtat
    20641 attgccaaac gccttaatct cgatgcctat ggctgtcggg cggataagcg cttttatcaa
    20701 aatatcaagc aaatggaaca gcgcgaatgg ctggccaaaa ttaaggcttg gctagatata
    20761 aacctgggat cagagccacg ttttagcggt gatatttttg accttgaggg tgatggccga
    20821 caaacttggg ataaattata attaaattta tgatgtctga agagcgcctg cttaatccgc
    20881 aggaaacaac cgaagaagcc gttttagaca agtcgttgcg gccgcgtttt ttgcgtgaat
    20941 acattgggca agaaaggatt aaggctaatc tggaaattgc cctttcggcc gccaagcaaa
    21001 ggaaagaacc ggtggaacat attttattgt acggcgcccc tggattaggg aaaactactt
    21061 tggctcatgt gattgccaat gagatgggag ccaatattcg cgttacttcc ggcccggcaa
    21121 ttgaaaaaag tggtgatttg gcggcaattc taagtaattt agaagaagga gatattctgt
    21181 ttattgatga aattcatcgt ttacccaaaa cagttgaaga aatattatat ccggcgatgg
    21241 aggattatgc tttagatatt attatgggta aaggaccgag cgcgcggact ttaaggcttg
    21301 atttgcctcg cttcactatc atcggcgcta cgactttagc cagcctttta tctgctcctt
    21361 tgcgcgatcg tttcggactt tcctatcatt tagattttta tctgccaaca gaaatggaaa
    21421 aaattatcgt tagaagtgcc catattttag caatcgattt gccgccatca gccgccaccg
    21481 ctattgccgg ccgttctcgg cgtacgcccc gtattgctaa tcgtctgctc aaaagggtgc
    21541 gcgattattg ccaggtgaaa aatcagggag aaatcagtct tgaagcctgc gatcaagctt
    21601 ttgctttatt ggcggttgat tatttaggtc tagatgcggt tgatcgtaaa attttacaag
    21661 ttttgattga taagtttaag ggcggaccgg ttggtcttaa cactttagcc gctgctaccg
    21721 gcgaagaagc tgatactttg gaaaatatct acgaacccta tcttttgcag ttgggattcc
    21781 ttaatcgttc gccacgcggt cggttagcga cagccgctgc ctatactcat ctgggtcgaa
    21841 atctttctga cggcggtctt ttatgaaaag catcaatttg tctttagaag attttaatta
    21901 taatttacct aagcatctca tcgctcaaac cccaatcaag ccgcgcgacc atgctcggct
    21961 tttgtgttta ggccgtttca acggtaacat tcgtcatcgc cgttttgacg atttaaaaga
    22021 tgagttgcaa ccgggagatg ttttggtcat taatgattca aaggtttttc cggcgcgttt
    22081 aatgggtcgt aaggacggta gcggtggcca agtggaaatt tttttacata aatttattaa
    22141 agatggacgt tgggagtgtt tggttggcgg gcgtgttcat ccaaatttaa aaattatctt
    22201 tagtcacgga ctatgggcgg ttttagaaaa agatcaagga gacggcacgt ggttagtatt
    22261 ttttagtaaa agcgggcaag aattttggca tttacttgac cagattggtc aggtgccttt
    22321 acccccatat attcaaggga gtggtcggca gaagttagac cgtcttcgtt atcaaactgt
    22381 ttatgctcaa gataatcata agggctcagt ggcggcgcca acggccggtc tgcatttcac
    22441 taacagaatg ctcgcaaaat tacggcaaca aggggttaat atagtgccgg taactctgca
    22501 tgtcggcttg ggcacatttg ccgccgtgaa agaaacggat ataaccaagc atcaaatgca
    22561 ctccgagttt atgtctatca gtgctgaggc cactaaagta ttggtggcgg ccaaaaagca
    22621 aaagcggaga atagtggcgg ttggaaccac tagctgtcgt gttctggagt cttatgggca
    22681 agaaattagt cggggaagat tatctttggg agagacgtat caaggatgga ccaatatttt
    22741 catttatcct ggctatcagt ttattttaac tgatgcctta attaccaatt ttcatctacc
    22801 taaatctagt cttttgatgc ttgtttccgc ttttgccgga cagaaggccg ttatggaggc
    22861 atatcgccaa gccattcagg aaaattatcg tttttacagt tatggtgacg cgatgtttat
    22921 tggctaataa ttgaaattta atcgatttgc tttttttact tttttgtgat atgctctcta
    22981 agcgttgcta accgtcagcc ttatttatta ggaatatggc ggcgcttgtt agcaattttt
    23041 cattatgttt aagaaaatac tcattttatt ttttgaagca gttaaggtgg tagcaatttc
    23101 tttagctatc attattccgg tgcgttattt ccttattcag cctttttatg taaaaggaat
    23161 gtcgatggaa tcaagttttc aagatcatga atacctgata attgatgaaa tcagctatcg
    23221 ttttcgtccg atcgctagag gcgaggttat tgttttccgc tatcccctga atcctcagga
    23281 atattttata aaaagagtca ttggtttgcc tggggaaagt gtgcagatta aagactcgca
    23341 agtaattatt tataacgatc aatatcctgg aggtttggtt ttgtcagagc cgtatttaga
    23401 caaagggacg ccgacctact ccaatagcga tgataagatt agcttgggtg cagatgagta
    23461 tttcgtcttg ggcgacaatc gtaccgcctc caaagattcg cgcagtttcg gtgcggttaa
    23521 tcgcagtttt attaccggga gagtgttctt gcgcggctgg cctttcagcc ggatagctat
    23581 ttttaatcag ccggtagatt acagtgattc taaataatta ttattttttc tatgtctcga
    23641 cagaaggtaa acgctaaaaa catgccgatt tttaaaaaag ctttagaaag aagaagtgca
    23701 aaagccattc ctcgcctttt aggcatgaag gatattgtgc cttcagaatt ccgttatttt
    23761 agcgctgttt ccaataaggc cgcagcttta gctgagcttt atagttttaa tgccatcaag
    23821 actccgatta ttgagagcgc tgatttatat aaaaaatcga ccaggcacaa taacaaagaa
    23881 ctgtatacac tcgactgcga aaaaggagaa aatggcgctt tgcgtccaga acttacccaa
    23941 ggaattttaa gagcttatat tgaaaataat ttttctgaac tccctcagcc tgtccgtctt
    24001 tattctattg gttcagtctt tagaaaagaa aaaatgcaaa ctggtcgtta tcgcgagtca
    24061 actcagttta atcttgaggt tatcggcgaa gacaagccga tggccgaggc tctattgatt
    24121 accgctgctt ataatttttt tcaagattta caagctagtt ttcagattca aatcaacagt
    24181 ttaggtaaag ccgattgccg caaagaatac atcagcaagc ttaatagttt ttataaggaa
    24241 cgtggtcgcc gttccaagct ctgtaataat tgtaagaaat atttaggcaa taacatgatg
    24301 gctttattgg attgcaaaga agaggcttgt cagaatttat tacatgaagc gcctcagatt
    24361 gccgattgtt tatccgatga aagtcggaat cattttgcta aagtgcttga atatttagac
    24421 gagctcggta tcccttataa ttttaaccct tacctggtgc gcggcctcag ttattataat
    24481 gatacggtct ttgagttttg gcctcttaat gatgaagggg ttgcttctag taaattagct
    24541 ttagctggag gcggccgtta tgatacttta gttgaaagcc tgggcggtcg accgacacct
    24601 tctgttggcc tggctattgg tttggaaaga ctggtagcca aggtaaaaga tcgcttaact
    24661 ctacccaact ttaaagagga ggtaatattc cttgcccaac ttggagatca ggcgaagatt
    24721 aaatctttga tactttttga ggaattacgt cgggccggat tcaatgttcg tcagtctttt
    24781 tccactgaca gtctaaaaaa tcaaatggaa gaagcggttc gtttaaatgc aaagataagc
    24841 ttgattttag gtaaaaaaga agtaatggat gaaactatta tgttaagaga catggagtct
    24901 ggagtccaag aaaccgtggc ctttaagaag ataaaagata ggttagaaaa aaaagtaaat
    24961 agaaaggaag gtgtaattta tggctgattt taaaagaaaa cctggcgaaa gttttgagag
    25021 tttcttgcgt aaattcaaga aaggcttaaa aaatagtaaa cgcctggaga aagcacgttc
    25081 caaacaacac ttggaaccca agaagactaa aagacaacag aagaaatatg ctttaatcag
    25141 tatcaagacc cagcgtaaaa aagaatattt acgtaagatc ggtaaattag aagaaactca
    25201 gaacagataa tatttataaa aatcgccctg tttagtgggc gattttttaa aagggcagaa
    25261 aaccccgcag gggctgccag taagcgcggg aaggggacca ttgagatcat taatattatt
    25321 gtctgcggtt ttattctttt tttctgcttg ggagaaaacg cttaaaattt tcaatcggac
    25381 tttttttggc aaattgcttg atacagtcac gcgctctttt tgttttgaca aattttagcc
    25441 aatcacgatt aggatattgc cgccctttat cggtgattat ttccactaaa tcgccgtttt
    25501 ttaattcttg gtcaagctta gccaagcgat cattaataat cgcgccggta gcttggttgc
    25561 ctatattagt atggacggca tatgcaaaat ctatgggagt ggaatattcc ggtaattcaa
    25621 aaacatcgcc ttttggcgaa aacacaaaga tgcggtcatt aaaaatattt aatttgatat
    25681 tttttattaa ttcactggtg tcggaaattt ctttttggat gtcaacaatt tctttaaccc
    25741 aatgaggttg taattcgttt tggtttttag ctttataata ccaatgggcg gctaaaccgt
    25801 ataatgactc ttcgtgcatt tcttgggttc tgatttgaaa ttcaaccgcc ttgccttccg
    25861 gtccaaaaat cgtagtatgt aaagaacgat agccgttagg cttggggacg gcgatataat
    25921 ctttgaaacg gttaggcttt ggtctccaga tagtatggat tatgccgaga actttatagc
    25981 aatcatcaat agtgggcaca attattctta aagcgaagac atcataaatt tcatcgaatt
    26041 ttctgtcttt gcgctgcatt ttttgataaa tgctgtatag atgtttgaaa cgaacttcta
    26101 aatcatattt taaattagtt tcggctaatc gtggcaatag ttctttctta attttttgaa
    26161 aatattggtt tctttccgct tttttttcaa cttcatattt ctgttctaat tcttggaatt
    26221 tttctgggta taagtatttg aagcagaggt cctctaattg ccactttaaa cgccagatgc
    26281 caagcatgcc agcaatgggt gcatatattt ccagggtttc ttgggcaatt cttttttgtt
    26341 tagcctcagg cagtccttca agggtgcgca aattatgcag gcggtcgcag aatttaataa
    26401 aaattactct gatatcagtg gccattgcta agaacatctt ttttagattt tctcggtagc
    26461 gctcaattcc cctgtatttt atttttccca gtttagtaac gccctcaaca agagtcgcca
    26521 cttcttcgcc aaagtttttt cgaacatctt caatagtctt atccgtatct tcgggaacat
    26581 catgaattag cccggcaatt attgttggta ggtcggcttt gatttctgtt aagagaaagg
    26641 ctgtatgtag tgggtgttgg atgtagggtt cgccggtttt acgcagttgt cctgcgtgcg
    26701 cttcaagcgc aaaatcataa gctaaacgca gctttgtggt atcttcgttg ggatgatttt
    26761 cctcaaattt tttgatgatg cgatcaatcg tcatgtcttt tctaattttg tatttttatg
    26821 ttataataag cttaactgct aagcttttaa attatatcac gcttggcggt tcaattaaaa
    26881 taaaatgcgc cgtcgatttt tttcgttttt attatttttt gcgcttatta gcgctgggtt
    26941 attctcggtt aattttgttt ttgcccaaaa tttagatgtc ggcattaacg aaattaacaa
    27001 tgccattgtt ttaagtgata ctgatcctcg cattatcatt gccaggatca ttaatatcgc
    27061 cttattgttt ttaggcgtaa ttgcggtcgg cttgatcatc tatgccggtt ttctttggat
    27121 gacaagcgga ggtaatgaag ataaaattga taaagctaag aatactttaa aaaacgctat
    27181 tatcggctta gtgataattt tatcggcttg gggtattgtt tcttttatta tgagtcgtct
    27241 tttaggcgct accggtaacg gtggcttttt taatggcgat tcgggcagca atagtgctct
    27301 agttggcact ggggcaatag gggcttgtac ggttgagcgt gtttatcctg aagatggtca
    27361 aaccgatgtg gctcgtaaca cttctataat cattactttt aaggaaccaa tacttttaac
    27421 cagtgtttgc cagaatgccg ctggcgaagc ttgtgcttgc gaccaggctt catgtaatct
    27481 aatcaatcct acacatattc gtattttccg gcaagatttt ggtgataatt gtggtgatac
    27541 ctcttgtcca aatgataata ctaatgtcaa tcaagcacac gtgagtgttt ccagtgaccg
    27601 gcagactcta attatcaccc cgcatgactt cttgggcagc cctgatggtg atactgatta
    27661 tcaagttaaa tttactaatg gtattttgaa ggatagcggt gattcaatct ttaaaacttg
    27721 taataccgat tggcttcagt ggggctttcg agtgagtaat aatttagatt taactcctcc
    27781 tcaggtgctg agaggaggca ttttcccttt gcctgataat gaaaaggatt tttacagtca
    27841 gactgttgcc gccgttgctg ctcaggcggc cgtgaccgtc aataattgtc ctcaggttta
    27901 tcaagcgccc tctattttag gagtaatacc tatagccggt aatcagaacg gtagcgctat
    27961 tcttgatgat aattttcatc agaatgtttc tgccttgacc gtcgttacta cccctgataa
    28021 caatcaagcc cagttgtttg ccgggcaaga attattggga gtagctaatt ggaacggcaa
    28081 tcagattgtt ttctctggat tttttcaact cgatgttgag ggtcatgagg ccggcaatgc
    28141 ttggcagata aatattcaac cggagatttt agccgaccaa cttactgtgg gcggagaaat
    28201 atataccttt accttttctg aaaataataa cgaccataat atttcgatta gtggttgtag
    28261 tggtttaaat attatcgccc ttaatatttt tgtaaaatta agcggtcatc ccgatgtcaa
    28321 tgttgattta gaagggaata aggtaatttt aatcgctaaa gtagccggtg cggctggcaa
    28381 taatataaat ttatctacca ctaatagtga cgccctttct ttgcaggcgt ttagcggcgg
    28441 gactgatttg gttagggtca gcgaagtaaa agatcgtcat gaccgtccga tgaattcggt
    28501 tattcaggtg aactttaacg aagcggttaa tccgatgttt gtttccggtt ccgctgatga
    28561 agtcgctgat tatatccggg ttgtcaatgc ggaagctaca gccaccgatg gtgcttcttg
    28621 tagcgttgac gccgactgcg cttcttataa gtgtagcgat aacgtttgtg ttggccatta
    28681 tctttctgga aattttttaa tctccaacgc ctacaagtca gttgaattta tttctgatgt
    28741 tgagtgcgga caaaatagtt gcggcgagaa gatttattgt ttaccagcgg atagtcattt
    28801 agccgttgaa ctggtagctg ctaatttaaa gacttgtaat actgatgcgg attgcgctaa
    28861 ttttcagccg ttttcccact gtgcgccatt ttttaattat cttacttgtc aggatgttaa
    28921 cggcaaaaat tatccggtgg ctaatcttga tcagcttgat ggcattgtcg acgctgccgt
    28981 taattcctta gacggtaatc gtgacgtttt tgcggatgga ccaattactt tttataacga
    29041 aaatgagccc gccaatctgg agcttaaaga taaatatcgc tggtcttttt atattaatga
    29101 tcaaataatg tccgcgccgc ctcaaatttc tttcattaag ccagataata atagtttaaa
    29161 ggctgacact aaagcccctg ttcaaattaa ttttaatacc ctaatgatga acgccagtct
    29221 gcgcacgggc agtgtttgga taaataacgg ccagaaaaac gtagagcatc atctcattaa
    29281 tctttttacc tcttctcctt cgccagtggg ttattgggtg acggcggaga atcgagatgt
    29341 ttttccgctt gacggtgaac ccgatttaac ttttgttttc ttgaagcata cggaattaag
    29401 cccttcggta acttataagt ctcaagtcgg ttcgggggta aaagatattt atcagaactg
    29461 tttcaagcct agtgccggcc ctaattgcgc ggctgatgcc aataatcctt cttgttgtta
    29521 cggtgtgccg actagccttt tgggggatga cggcaattgc cagtaattag ctaaagttta
    29581 gtaataaaat aaagtttaaa gtcgttagcg gttttggccg ctgattatct tcacgctttt
    29641 atgctgaata agtcttcttt tgtgaataaa aataagaaat ttattattcg gattgctgtc
    29701 ttcctctttt tgtttggtgt cagcttaacc tttagcggtt tggctaacgc tcagattgat
    29761 gtcggtgtta atgagattaa taatgatatt gttttaagtt cgggtgatcc gcggacaatc
    29821 gccgcgagag ttattaatat cgtcatgctt gtcttgggag tgctggctgt cggtttgatt
    29881 atctacggcg gttttatttg gatgacatcc aatggcgcag aagacaagat agaaaaggca
    29941 aaaaaaattt taaaaaacag tgtaattggt ttagtgataa ttttatcagc ttggggtatt
    30001 gcttctttta tattaagccg ccttacgggc gcaactaacg gcaatggttc atctggatca
    30061 tcatgtaatt ctggacaatt attgtcttgc ggttgcggtg gctctatgag ttgtattgac
    30121 ggttcttggg ggccatgtct ggggtctgat tgtaataatg gcggcggccc gagcagttgt
    30181 gacagtagtc ctttaagtgg ctgccaagcg gctgatcaaa tctgttctgt tgatagtttt
    30241 tgcgatgtgg atatttgctc atgtttgcct aaaggcgaat tgggagacta ttgcgatgcc
    30301 gatactaata ctccaacctg cgaagcggat aataataaat gcgcggaata tttaagctgc
    30361 aacccggact cttgtacttg ttacgggccg ccggtgatta ccaatattag tcctgcgggt
    30421 ggtttttgta atgagaatgt tgatttgtca tgcactaccg acgccgattg ttctttgggc
    30481 tgtaatttaa acacgcctaa cggtacggct gataatatca ttactatctc tgggtctaat
    30541 tttggccagt atgaccccct agtgagcaag gttattttta gtacggatgc tgtcggcctt
    30601 aatcctactg ctttaaatcc tgattgtatt gattcttgga caaataatca gattatcgtt
    30661 gttgtcccga atacagccac aactggggcg gtgagggttg ttaccgctga taatcagagt
    30721 gatagcacta atgacgataa tggaccgatt attgaagatt ttgtagttaa cagcattgtt
    30781 cgccctggag tttgtttgct cactccggac tctgggttgt taagtgaaaa ggtaaattat
    30841 cgcggtgtta atttgtatgg aggcgaagct tattttggta gctatgataa aaatgtgcgc
    30901 ggtttggctt ccgattttat taatccgacc ggcttaaccg gcacaactac
//
LOCUS       DAOQSA010000008        29112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_964_length_29112_cov_7.791169, whole genome shotgun sequence.
ACCESSION   DAOQSA010000008 DAOQSA010000000
VERSION     DAOQSA010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 29112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29112
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_964_length_29112_cov_7.791169"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..1077
                     /locus_tag="PKK65_01455"
     CDS             94..1077
                     /locus_tag="PKK65_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HNU81381.1"
                     /translation="MRTRNTTDNLIAMKTSILDLCIARRMSCTLGAQTLKMHIKSFSR
                     LKSRYQKYGKDVLIPKKPGPKRFKPANRTSDDVARVVCDLAVKRPDLGPIALRDELEE
                     LNIHLHPTTVWRILKRNQIRYTTTYKRWKPDPKLYCLETPGEELQLDACYPYGRSRKL
                     TCFDAIDDCSRKIFARLYDRETIENAIMFVSDLVKNSPFRIQRIRADNRYKSKLFINH
                     CNSLGIEVIINEPYTPEQNGKIERFHKTLKRSFFWKYCSFYDSNEYLQLKLNSWVNFY
                     NSKRKHYGYGMNGMTPDLKIASSLFNSLNIINTYPQKVTLILQQYIFSLFC"
     gene            1103..2566
                     /locus_tag="PKK65_01460"
     CDS             1103..2566
                     /locus_tag="PKK65_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNU81382.1"
                     /translation="MAEEVIIRFDEVTFEYLAKKPVLKEASFSVRRGAKITLMGQNGA
                     GKSTIFSLLKGEIHPQSGKISLTKQASIGAARQTMARAHLPLSVEDYFATAFKEKPAN
                     LRSRISKVLEAVNLEVPFEKIVGELSGGQQARLLLAYALIQDPDILLLDEPTNNLDKE
                     GIDHLLTFLIMYDKTVIVISHDADFLNCFTEGVIYLDVFTHQTEMYVGNYYAVVEEIN
                     RRIEREIKKNAQLEKKIIDRKEKVNFFAHKGGKMRKLAQKMKAETEELEENMVDVRRE
                     DKTIRNFNIPVQADLSGEIVRLNKVKVIKNHEPVFCEVNKVLTRRTRLLVTGPNGIGK
                     STFLRALVNGTQEGISIVKGVKVGYYSQDFSNLPYDQTAYQFLAADLLAGSGEQELRS
                     LAAGFLLTGEVLALPVAALSEGQKGLLAFAHLVMLAPGLLILDEPTNHLNFRHIPVIA
                     QALNEYEGAIIMVSHMPEFVKQVRMDEELDLGKLGEK"
     gene            complement(2624..3142)
                     /locus_tag="PKK65_01465"
     CDS             complement(2624..3142)
                     /locus_tag="PKK65_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81383.1"
                     /translation="MKKLSKKQFFLAISAGILLLILLAWLKERGWILFPEIEKVRLYK
                     NQTYALGAPEFFKLPRPYSRFWDIFFLPIFIYALAVISKKTEANLWILTGEMLIILAL
                     SHYAASTVVAIYAGMLFAAVGGLVFNEFVGIFLALIFGIIVGFAAFGLLFGLLLAGIA
                     YLLYQVIKFFIG"
     gene            3346..3504
                     /locus_tag="PKK65_01470"
     CDS             3346..3504
                     /locus_tag="PKK65_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81384.1"
                     /translation="MSSLNSIIKRLHELTLKKGELLSLFRAKVQEAELKAVKEEIAKM
                     SVSKDDKI"
     gene            3509..>5112
                     /locus_tag="PKK65_01475"
     CDS             3509..>5112
                     /locus_tag="PKK65_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81385.1"
                     /translation="MEKLEKFIPEISDKTQTNESETEKSSTMKARLRQLFAFFHSKDQ
                     EVAAIENSAKHLEPEEISDIKEELRLEEKLKVINTEAQALEKEALELEKPEPKEISPA
                     ETYFNQELLPFLKVIASGEELSRLVSELRLAAEKKLAKNKGKKTEEKTIATMKQLLID
                     RCRETIVCAEKFKKSGKGQSPDPDLLLRSKIITLAAGDTKALEKYSLLGYKERHEALL
                     NGYLNFERSYYSNSKIDADAIHLLMSAGFKDDFLKLRPNERRDIISEYDNQLIVAIID
                     SNREKNGDRSDLYDELGYAMACQSKECSREVYEKMVACGALYYVIKHLDKFQRVVSPA
                     DLSDFARHYSTAYPYMLDDLFEGIEKTDWTEEEKMEARRNVLKFHNVTDSNRRYYFNR
                     EEMKEFKDIADNIKELDLAEDIPGIKRELEMGRLPYFFECRHGFKTLIENEPEYLIAL
                     ASRYDQEHLLIHRLSDPYLQELKKNYVEKLARQGDLDIISELEKKGELSAVISKELYL
                     SLADQQGYVADLDCAINVDDEIFARY"
     assembly_gap    5113..5212
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5213..8471
                     /locus_tag="PKK65_01480"
     CDS             <5213..8471
                     /locus_tag="PKK65_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81386.1"
                     /translation="EKQKIISFIIRFGVDALTYKPENFQSLLNEAKELPSSVWAESAE
                     RFRNTAEFVREMEGEEKRRFLLDLQIAASILGKQDTQFVNFFLKKEGVNTGANIASHD
                     LFDAAFRYSDGFASLDEAKQKVLRKIIPGIDRVVSLREKLKESSSETEETLRKKLADS
                     FGFLIKNWKDDDLEKFCGYVKEFGNKPFIQLFSLYEQISQCEPATLPLEVRARLLKLT
                     GLKINKTETSEQLLQRLNEKISAIVPEVDQGQARQCAISTFTKLRLDALNGKYQDAAS
                     GYTAPFLRGELREEAANGDPDRFSSFNYSSLVEGRANNQWAMNLWLYIYGTEGALGDK
                     AVEMAKMFDNAEYRDEVLLKYKNEWLENLSSGDSRLNLKLDSICSTVNYFGGAGNLKH
                     IESLSNLITNIRDHAWGNPHTVERTKKEIETLLIEQEKRFDKEKWSLDARSDFYNLSA
                     DIIEAAPSLYSSFQPLFDRLDGKEMKQFARECLPLYQAKLVAMQSQDLKYKPKELVTL
                     RNAITVLAAKLELYPEDKQENLAEHKQELLDDLQAKFQTRFGINHIPEEINKESIRTI
                     SNCIRYLGNISRRNQKSEALISLYLSLSLNNEWKDFRAGKDIDINKHLIPACAQNIAA
                     MIEKKRENTLPYALVDIAPEEVPEFQRILQSEVSSTMLGNVQTIDYKLLNLKHNIKDL
                     ADPDAFPEATDKQLLEICRGGSKSLNALLAKVYGETVGKTTVYNESEAKLKDQLANIY
                     GIKSWDATAVKSIQDRVRPMNLIFNLLDKMEKEQLDLRVSELRDSLKPSPAIIDIYNR
                     LEEGFKPESGAYASFRDLEYLEGLAVKAESQLSDQEKKDIESYLAPIREKMAGLERLM
                     TRVKEYFNKLSDVANKSNQPMLKERLKDITTAFADSEGESIVQSYLTSDLNLIIENMR
                     QCLGCLRKEINNDTNLSFGDYNKFFLMSRQGKDEGSIADQIVFLAPVKNLNTGEQELN
                     FILDRIYGNKTSDLLVAHTLAVHKKYRSLKDKFKNAKLGVIVSNMALFSVGINQDDFI
                     KKLKEKAALAAEPVFEERLEADIKESAFSDHYVEFADHEYGRKKGALSFSGVMIK"
     gene            8629..10053
                     /locus_tag="PKK65_01485"
     CDS             8629..10053
                     /locus_tag="PKK65_01485"
                     /inference="COORDINATES: protein motif:HMM:NF014605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNU81387.1"
                     /translation="MDRHNKIPGSKNSPANQELQQLLASRAHKNIVIPDTNVIVQDPD
                     SVREFLKGGNLLVIPWQVFLELDNLKKSSEVGWEAGKGIKNIHDLKMAQANLVIERRT
                     YFPSGNDLNKFEPDHRIIATALYVVRNLTVPGSQYRGYSKVKLVTNDYGMQIVASEFI
                     KRDDFCFEPYKRDLIKLKEESLIMPVKKILQEEIKKNENRQEYCEIGQREKIPYGRPV
                     MIHLKLGETSSPYAVALRRDNRLEFLDSNLKLSGIGAKNNGQPNWQQIAALHLLLDQS
                     VSAVFLQGGAGTGKTLLALAAGIHQRDQKLFDHLIIMRPTIYLSEDDNLGFLPGDINN
                     KLAPWILSIRHNLAEIKRTPKNAKIDEDDQSSLDFLVKYGVEIQPLGYIRGASFKRCF
                     IIIEEAQNLPRHLIKTILTRPAEGTKIVFTGDLGQIDNKRLTRESSGLAYAINKMSDN
                     QMIGIVNFEQTLRSPLASLADKIL"
     gene            10240..10902
                     /locus_tag="PKK65_01490"
     CDS             10240..10902
                     /locus_tag="PKK65_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81388.1"
                     /translation="MKKLIVFLFIFCFSLFQISISLKEGIESARADSFKTVTVTFKVA
                     PTPASVEVNKTLVKYNKDFIFSWGFDDGYKEGYEYGFKYLNGGYNSTLDSYFGGLYYT
                     DGAGSSRPFSGSYSWFSVDSSYNDLHSSDSLSYMSWDNLIDSYNHGWVALNHGWTSMN
                     FPDLEATYNYPAPKGPGSFDYNYEVSQNSSYIQEKTGFYPKHFVIPGGVSHISARHSM
                     SV"
     gene            10899..14999
                     /locus_tag="PKK65_01495"
     CDS             10899..14999
                     /locus_tag="PKK65_01495"
                     /inference="COORDINATES: protein motif:HMM:NF039533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNU81389.1"
                     /translation="MKSVSAQAINFNYDGKNHLVPVEGVPLSSLNLNEYLMYRKYYAE
                     GINFDLSSMTEYVDAIAAKSTGAEKYWAVGFTHRVSPDATSGNLSWDKFKYLMDYIAA
                     NYGRDGADNIWMTSYQEAYEYAEVYQGVSLQTSLSGDTLTISFDTSTLPGDLRYYALS
                     LLVSADQEIRSVDYGLGDFTATSANLDSGLINLDWGFFLDQDEYTRSEEEVSKAEVSR
                     ISNDRDKAQSYVNVLPDGQRKNILQNRIDNIAVLGKTWRINFGLNSYIVGTWNIYGGD
                     KSPYNKLLENLVDTDGNVSSLSVQVVQDFTSKFLNKGMNTGNNSGVIPDAYLQTGLSM
                     YASTQQTGIVRFGGLDPAKKYDVFIVGSYNNIASSSKSISLYTIKGETKELNIAQNTA
                     NGVTFHEISPEPNGSLDVAVSAKDSYFGYGVLNAVEIRERSQNKPSALINSITLRANS
                     GLVDLNYSLIHDDADPIDLEYLQYSITGFFAGEELTALSASDPQNNGISNLASSITGT
                     NHCFVWDARTDLGEFEGEAYLRFQPRGIHQSGAFIQTDAINLDFKAPIFSNLTISSSE
                     NSAEFSWHTHEDAYSRLDYGLSSAYGLSVGSLDSGYFFRDPDYSLGGLHACAAYHYRL
                     LGRDAANNERQSDDAYFLTDGCLGGQKPVSILESSFSSSESKDLSLDGLRLLLPSNAI
                     SGSPILQIFRLDANSAIASAPLPESSHLLGDKIFSLNAYSSLSTKQSQLGGSATIIIS
                     YDQVDIFGLDPSTLRIAYWNGSAWDNLSDCSVNQSEREVSCTTDHFSDFALLGDESIP
                     GEPLLLSPNGAGVIVSGSSTQQILWQIPDDDDLSHFDLEYSSDNGASWMGLVSGLAST
                     TDTYSWFVPPLNTAEALLKVTVYDLDLHSSSSQSSVAFVIDTTNPTVTTPNLGAINIA
                     TAAGATAADTNGIASYSWTKVSGPGTITFSASSNILNPTIAASTDGSYSAQLTVVDNA
                     GNTNNSIITFIWDTTAPSITIAPIFLDPTGDTTPIFTGTTTDALINISVVEYQVDGGP
                     WTACSATDGAFNSLSENYTCTVASSLSDGLHTVYVRASDTLGNTSDGSVSDSFTVDLS
                     GDEGEVLDGGDSDEDEDDKDVQSVTAGTPINISPPQTPAGGFIKNGLSGLNGTFSYFL
                     KQNVKTFDLELQAGDDVDRYSLSVDPEFKNASIFPYFSPLRLDICSFFENCHDGIYTL
                     YLRFYTRNGIASDNIRLDIDYRGGDEVKLEEENDKKDKISPGILAKRLAGRILLQVES
                     RGEAWYVSPEDYHRYYLRDGNAAYELMRKMGRGITNKNLERLRREADFARRFKGYIFL
                     QVESHGEAWYLDDDLNLHYLRDGNAAYELMTSLGLGITNKDLELIP"
     gene            15003..16103
                     /locus_tag="PKK65_01500"
     CDS             15003..16103
                     /locus_tag="PKK65_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNU81390.1"
                     /translation="MFAFFSILVFLFLVISGHYFAWHLCLSAFPSWHRWRFWILSAFV
                     AVTFIFLGDFIWLHQGDSAFFRYLYVFGAILFGLLSQLMLFGFLFYLHEILLKHWTGK
                     RLRCASLQPRLVARLLLILAGLFFILGTYNAFLPRVKTIVLRGWPEELKGKSFVQLSD
                     LHLGAVYRPAWLEMIVRKVNALNPDFVVISGDLFDGSDEHLSEFIPVLRTFSPVTIFV
                     PGNHDYYIPAEKAQETALAGNLIFLSDAAINVDGLEVIGLNYVNRDDSGLRREIKNLG
                     DGQLTRIVVNHVPVDQAEAKALEARLMLSGHTHRGQIFPFSFFTYLLYGRFSYGLENY
                     EGMTTYTNAGTGTWEPPVRTLLPGEIIRFVIE"
     gene            16264..16701
                     /locus_tag="PKK65_01505"
     CDS             16264..16701
                     /locus_tag="PKK65_01505"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNU81391.1"
                     /translation="MSQPELTNLINSIRARGRRLSGGREALLRALATANKPLSAAAIM
                     RSVAVKRANLDRATVYRTLSFWEKEGVVEILHLEGDERLYHLNLHHHHHLVCTVCKKI
                     SAVDFCQSLSREEAAIAKKTGFKIQRHTLEFYGLCRECARKNY"
     gene            16726..17700
                     /locus_tag="PKK65_01510"
     CDS             16726..17700
                     /locus_tag="PKK65_01510"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HNU81392.1"
                     /translation="MPKRFIFMGIFALIIAAFAWTAADSWQKKQLSKDTPILDKVQET
                     TVASEASPIIVVSSLFPWYDLSREIGGDRVNAKLILPPGVEAHAFEPTPGDMISISQA
                     DLFVYTGDYLEPWAQDILAGLNGSAPESLAIGKDLAELPKAAPEEAGLDPHIWLDPMI
                     MFKAAGRLAQTLAKIDPDNKAYYYERLENYQVRLEELDANYRQTLSTCKQQEFIYAGH
                     YAFGYLAARYGLQYQAAQGFSPDAESSPQALSELLKSLKAESADYIFAEELDNPQLAK
                     SLAKDAGVKILMLNSAHNVSKEDLKAGLTYEEIMKNNLKELSLGLKCQ"
     gene            17691..18437
                     /locus_tag="PKK65_01515"
     CDS             17691..18437
                     /locus_tag="PKK65_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HNU81393.1"
                     /translation="MPVIVEVKNLDIVYNSRPVVRDVSFSVSAGDYIGLVGPNGAGKT
                     SLVKALFNLLPVRRGEIKLFGQSLNTFKQWERLAYLPQKHQYNRLFPASVLEVVLSGL
                     AGRRRRYDKTSAKMAALSLLEELAMADKADKLFSRLSGGQQQRVLLARALIRKPELLI
                     LDEPTTALDPMVREKFFTLLAKANQEQGTAIIMVTHDTADIGRYARLLLYLDEGLVFA
                     GPFADFGHSEKMKKYFGDFAQPLSSHQPDH"
     gene            18443..19255
                     /locus_tag="PKK65_01520"
     CDS             18443..19255
                     /locus_tag="PKK65_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNU81394.1"
                     /translation="MEFLSFFQYPFIIRALIAGILVALPAALLGLFLVLRKLSLIGDG
                     LSHLSFGAVALGLLCGVYPFYVSVPLTVAGAFLIFRLGNKARIYGDAAIGIVSAVGVA
                     GGVIIASLAKGFNVDLFSYLFGSLLAVSWGEIYFLALLAAATLVFIFIYYRSLFAWAF
                     DENSARAAGVRVDFLNNVLLILTALTVAAAVKAVGVMLVSALLILPAASALQGAKHFR
                     SALISASFFAFIAVIIGLAAAIYFNLPAGASIVIAGFFLFVLAYFRAKLLKV"
     gene            complement(19342..19608)
                     /locus_tag="PKK65_01525"
     CDS             complement(19342..19608)
                     /locus_tag="PKK65_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81395.1"
                     /translation="MDRNKELKKAFDLFACLLEKTNNKEIVDDTINEFLDFIEKINDP
                     VLAANFQSYWLDIREKIKAKADKNHFVIQERLIKLNLERIMSSK"
     gene            19657..20184
                     /locus_tag="PKK65_01530"
     CDS             19657..20184
                     /locus_tag="PKK65_01530"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNU81396.1"
                     /translation="MSNRRRGLDLKKILRIDYNDFMNNITLIAVGALKNKALQSLAAD
                     YQKRIKPYARLQKLETPASPFRKDDRNKAKKREKEALEKVLHRYPKESIFILAETGRL
                     FDSVAFSNFLSEYDGRDLVFLIGGALGWEEELALKYPALSLSPLTFPHELARVVLLEQ
                     IYRGLLLKAGKEYHY"
     gene            20238..20924
                     /locus_tag="PKK65_01535"
     CDS             20238..20924
                     /locus_tag="PKK65_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81397.1"
                     /translation="MISPAKKIIPVFNKQQRRLVWTMAVLTVIAAGLLLVFSWRLSRL
                     NFLPAWSSLSVLPALFQGETMRETAVATLPVFSSTEELSFLSSPDGRNFAYILNNNQS
                     QQLVLNEKAGPIVDSVTFMAFSPDSRSFAYTYKLKDKERAVINGLVGKEYDWIFNPRF
                     FTPDSRYFIYKAREKGKDRLVINSQESRLYDRIYEPFLSPDKATLIFFARDGERLWRG
                     EIPLERSGDQ"
     gene            21342..22073
                     /locus_tag="PKK65_01540"
     CDS             21342..22073
                     /locus_tag="PKK65_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNU81398.1"
                     /translation="MPVTFIQADKRLNAFFRSAYNREKAEILGRFFKTGPGQYGEGDK
                     FIGINVPTLRALAKDYQEIDDVFLEELLASPYHEIRLLGALIMTLRYERASTEKDRLG
                     ALNFYLNHHLALNNWDLVDLTVSKVWGDYLLHHPEKRKDLYRWGRSQNLWERRLAVVA
                     TLTLIKAGQFKEILDLSVMLLDDQEDLLHQAVGWMLREVGKKDKQVLVDFLLKYAARL
                     PRTTLRYAIEHFSETERKKYLNLKR"
     gene            22123..22893
                     /locus_tag="PKK65_01545"
     CDS             22123..22893
                     /locus_tag="PKK65_01545"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNU81399.1"
                     /translation="MPDINLFKSEAGAKAKPAIIIIGLVLLASIITVALLRDRLVNVQ
                     YRQVTVMGQGRVSYHPDLAVINLGVQIDKAKQAEEALKQLNEKVNKIIAAVQAEGIEA
                     ADISTQNYNLYPQYDYQDNISTVAGYSANEQLSIRIKDYDSHPERLNKVLAAAAKAGA
                     NQINGLSFDSSSLNTLKQQARILALRDAQAKSGELMAVTGVKIKNIAGWWENVIYPPS
                     VTYVGGDLGKGGAADYSAATPSGNREIVIEVGVNYNIK"
     gene            23230..23511
                     /locus_tag="PKK65_01550"
     CDS             23230..23511
                     /locus_tag="PKK65_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNU81400.1"
                     /translation="MSKMTKAQIVAAMSQATGLSKKDVNGFFETLSEMTYKEVKKNGE
                     CLVPGFGKLVKAKRKAREGRNPATGETINIPAKTVVKFRLAKAVKDAVL"
     gene            complement(23589..23876)
                     /locus_tag="PKK65_01555"
     CDS             complement(23589..23876)
                     /locus_tag="PKK65_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81401.1"
                     /translation="MLNYLRGLHVKNPIWLSVSEAAKLGGVTDKTIRRALKAEGDNLK
                     FRVSQNRYQIELSSLLIYLHRNTKLKNKLFAFGLGQYWPQAVMTSSRDINR"
     gene            24243..24842
                     /locus_tag="PKK65_01560"
     CDS             24243..24842
                     /locus_tag="PKK65_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNU81402.1"
                     /translation="MIIIMTPHIVKKFREKQLLQRLKSQDKEAFIKVYDDYVRDIYRF
                     IYFKVGKEEEAQDLTSMVFLKAWNHIQTNSLSDSKTLRALVYKIARTSIIDYYRESGN
                     KLEVSLNDEDKPIEIIDENQDMAADLDQSTNLELIRRQLPLLKEEYREVIVLRFINDL
                     NLEEIAAVTGKSRGNVRVILHRALAALKAMVEDKMEKNK"
     gene            complement(24993..>25183)
                     /locus_tag="PKK65_01565"
     CDS             complement(24993..>25183)
                     /locus_tag="PKK65_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81403.1"
                     /translation="KVRRAFLVAVSPYNFFYYGIVINKNPKKLWGEGFRHKESFYKYA
                     GGLVFENAKEKYVQIWPK"
     assembly_gap    25184..25283
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25284..25531)
                     /locus_tag="PKK65_01570"
     CDS             complement(<25284..25531)
                     /locus_tag="PKK65_01570"
                     /inference="COORDINATES: protein motif:HMM:NF024098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HNU81404.1"
                     /translation="MLVFIDESGDSGLKIEKGSSRYFTIGLLVFEDNEEALNCDQRIK
                     SLKKELGLPENYEFHCKRNSDKVRRAFLVAVSPYNFFY"
     gene            26873..27691
                     /locus_tag="PKK65_01575"
     CDS             26873..27691
                     /locus_tag="PKK65_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81405.1"
                     /translation="MKQLKGLKNISPAASWLKSNREILLSQIANSSAQELTPQRRLLI
                     DVRSFLAAVSRPSLVLGSFLLLLAGVSFFGHLAFSRTKPNESLYIARIISEKAKLNTV
                     FDSEERDKMEVQFATSHAEAITEVLAGTDVNDEDQVAKLNDSFNKELETVRTKIAVIK
                     KNNTQTVAVPSEIPAVSEEDILLTAGNGKDDAGMQIALNNSTTPLASPTAPVLAVDKV
                     TPAETATPTPEVVTEKEVVADKAEEVLNEAQNLFDHKEYSGALDKLKEVKEMIK"
     gene            28324..>29112
                     /locus_tag="PKK65_01580"
     CDS             28324..>29112
                     /locus_tag="PKK65_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81406.1"
                     /translation="MKKLRKFLTVGVMVLSIIAMSGLTVNTAQAAAQPGDLIKMDGLS
                     SVYYLGNDGKRYVFPSESVYFSWYNDFSGVITIPASELQSYPLGANVTMRPGTKLVKI
                     TTDPSVYAVTPNGVLRKIQSEADAIALYGPNWNKMVVDIADAFFTNYTIGTPLNSGEY
                     PAGTLLKNPNNASIYYFDGTNYRMISSEAAFNANRFNMNNVVTTSMTLTASGTAITGA
                     EDLAKPYGATSGSVVTGSGLTVALAATTPAAASVPAGAAIVPFTT"
BASE COUNT         8211 a         5554 c         6394 g         8753 t
ORIGIN      
        1 tgttacaatt gcagcaatta gggttacctt tcgggttccg aacccatata atatggtggc
       61 agggctattt ggcccataac taccataaac tatatgagaa caaggaatac cacggataac
      121 ctaatcgcta tgaagactag catattagat ctctgtattg ccaggagaat gagctgcact
      181 cttggagcgc aaactctgaa gatgcacatc aaatcatttt cgaggctaaa atctcgttac
      241 caaaaatatg gtaaagacgt tttaatcccg aagaaaccag ggccgaaaag gttcaagcca
      301 gccaaccgca cctcagatga tgttgctagg gtcgtgtgtg atttagcggt taaacggcca
      361 gaccttggtc ccattgccct aagagacgaa ctagaagaac taaatattca tctccatcca
      421 acgactgttt ggcgcattct caagagaaac caaatcagat ataccactac atacaaacgc
      481 tggaaaccag atccgaaact atattgccta gaaacaccgg gagaagagct gcaactggat
      541 gcctgttatc cttatgggcg ttcccgaaaa ctaacttgtt ttgacgctat cgatgattgt
      601 tccaggaaaa tatttgccag attgtatgac cgggaaacta tcgaaaacgc tattatgttt
      661 gtgagcgatc tagtgaagaa ttcacctttt cgcattcaga gaatcagagc cgacaacaga
      721 tacaaatcta aattatttat caatcactgt aactccctag gtattgaagt aattatcaac
      781 gaaccatata ccccggaaca gaacggcaag attgaaagat ttcacaagac acttaaacgc
      841 agttttttct ggaaatattg ttctttctat gattccaatg aatatctgca acttaaactt
      901 aattcttggg tgaatttcta taattccaaa cgaaaacatt atggttatgg aatgaatggc
      961 atgaccccag atttaaagat agcctcatct ttgtttaatt ctttaaatat tattaatact
     1021 tatccccaga aggtaacttt aattctgcaa cagtacattt tttctctttt ctgttaatct
     1081 aagggagaat taaattaaaa ttatggcaga agaagtaatc atcagatttg atgaagtcac
     1141 gtttgaatat ttagcgaaga agccggtttt aaaagaggct agtttcagcg tgcgccgggg
     1201 tgcgaaaatt actttgatgg ggcaaaatgg tgccggcaag agtacgattt tttctttgct
     1261 taaaggggaa atccatcctc aaagcggtaa aatttctttg actaaacagg ctagcatcgg
     1321 ggccgcccgc cagacgatgg cgcgcgcgca cttgccttta agcgtcgaag attattttgc
     1381 cactgctttt aaagaaaagc cggccaatct gcgtagccgt atcagcaagg ttttggaggc
     1441 ggttaattta gaggtgcctt ttgaaaagat tgtcggcgaa ttatccggcg gccagcaggc
     1501 gcgcctgctt ttggcttatg ccttaattca ggatccggat attcttttac tggacgaacc
     1561 gactaataat ttggataaag agggaattga ccatctgctt actttcttga tcatgtatga
     1621 taagaccgtg attgttatct cccatgacgc cgattttctt aattgtttta ccgaaggggt
     1681 tatttatctc gatgttttta cccatcagac ggaaatgtat gtcggcaact attatgcagt
     1741 ggtagaggaa atcaacagac gcatcgaaag ggaaatcaag aagaatgcac aattagaaaa
     1801 gaaaattatt gatcgtaagg aaaaagttaa tttttttgcc cataagggcg gcaagatgcg
     1861 caaattggcg cagaagatga aagcggagac ggaagaattg gaggaaaata tggtagatgt
     1921 ccggcgggaa gataaaacga ttagaaattt taatatcccg gttcaagcag atttaagcgg
     1981 agaaattgtt cgtctcaata aggtgaaagt gataaaaaat catgaaccgg tattttgtga
     2041 agttaataag gtcttaacgc gaagaacgcg tttgttagtt actggtccca acggtattgg
     2101 caagagcacg tttctgcgcg ctttagttaa cggtacgcag gaaggaatta gtattgttaa
     2161 gggggttaaa gtcggatatt atagtcagga tttttccaat ctgccttatg atcagacggc
     2221 ctatcagttt ttagccgctg atttgctggc gggttcgggc gaacaggaac ttcgttcttt
     2281 agcggctggt tttttgctta ccggagaagt cttagccttg ccggtggccg ctttgtccga
     2341 agggcagaaa gggcttttgg cttttgctca tttagtgatg ttggcgccgg gattattgat
     2401 tttagacgaa ccgaccaatc atctcaattt ccgccatatt cccgttatcg ctcaagcttt
     2461 gaacgagtat gagggcgcga ttatcatggt cagccatatg ccggaattcg tcaagcaggt
     2521 acgtatggat gaggaattag atttagggaa actgggagaa aaataatttt aaatttatat
     2581 ttaaaaggcc aaagaaaaac tccctatcgt ttgataagga gttttagccg atgaagaact
     2641 taattacttg atacagtaaa taagcgatgc ccgcaagaag caggccgaag agcaacccga
     2701 aggcggcaaa accgacaatt atcccaaaga taagggcgag aaatatgccc acaaattcat
     2761 taaagactag tccgccaacg gccgcaaaga gcatgccggc ataaatagcc actaccgttg
     2821 aagccgcata atgcgataat gctaaaatta tcagcatttc tccggttaat atccaaagat
     2881 tggcctcagt ttttttgctt atgacggcta aagcataaat aaatattggc agaaagaaaa
     2941 tatcccagaa acgcgaatat ggtcgtggta atttaaaaaa ttcaggcgcg cccagagcgt
     3001 atgtctggtt tttataaaga cggacctttt cgatttctgg aaataatatc caaccccttt
     3061 cttttaacca agccaataag attaaaagta gtatcccggc gctaatcgcc aggaaaaact
     3121 gtttttttga aagttttttc atggcgatta tttttttaaa ttgtaaagaa tataatatca
     3181 taatatatgt agtgtttttt gtaaagattg ataattttac aattaaaaaa tcgtcgtctc
     3241 tttaaatccg gtcaaaatcg cagctgcccg tttaagtaac gacaggtgac aaactggacc
     3301 actttaggta taatcaataa ggagctaaat aatttcaaat taacaatgtc gtctctaaat
     3361 tcaattataa aacgtttaca tgagctcact ttaaaaaagg gggaattatt atcattattt
     3421 cgggcaaagg ttcaggaggc cgaacttaag gctgtcaagg aggagatcgc gaagatgtcg
     3481 gtgagcaaag atgataaaat ctaattatat ggagaaatta gaaaaattta tccccgaaat
     3541 atcagataag acccagacaa atgaatccga gacggaaaaa tcctcaacaa tgaaggcgag
     3601 gttgcggcag ctcttcgctt tttttcatag taaggatcag gaggtagcgg cgatagaaaa
     3661 tagtgctaaa catcttgaac cggaagaaat ttcagacatc aaagaagaat tgcgccttga
     3721 agaaaaatta aaagtaatta ataccgaagc gcaagcctta gaaaaagaag cgcttgagtt
     3781 ggaaaaacct gagcccaaag aaataagccc tgcggagaca tattttaacc aggaactact
     3841 gccatttctt aaagtcatcg cttcgggcga agaattgtca cgtctggtgt cagaattaag
     3901 gctggctgcc gaaaaaaaac tggcgaagaa caagggtaag aaaacagagg aaaaaacaat
     3961 agccacgatg aagcagctgc taatcgatcg ctgtcgagag acaattgttt gcgcggagaa
     4021 gtttaaaaaa agcggaaagg gccagtctcc ggatcctgat ttattgcttc gttctaagat
     4081 aattaccttg gcggccggcg acaccaaagc cttagaaaaa tacagcttgc tgggatacaa
     4141 ggagcggcat gaggcattat taaacggtta tcttaatttt gaaagatcat attattcaaa
     4201 cagtaagatt gatgccgacg cgattcacct cttgatgtca gcaggcttta aagatgattt
     4261 tttgaaattg agaccgaacg aaagacgcga cattatttct gaatatgaca atcaattgat
     4321 cgttgccatt attgattcta atcgggagaa aaatggcgat cggtctgatt tatatgatga
     4381 actcggttac gccatggctt gtcagtctaa ggaatgttcg cgagaggtct atgagaagat
     4441 ggttgcttgc ggtgctttgt attacgtcat caaacatttg gataaattcc aaagagtggt
     4501 ctcgccagcg gatttatcgg attttgcccg tcattattcc actgcctatc cctatatgct
     4561 tgatgattta ttcgaaggca tagaaaaaac tgattggacg gaagaagaaa aaatggaggc
     4621 tcgacgcaac gtccttaaat ttcataatgt gactgatagt aatcgccgct actactttaa
     4681 tcgagaagag atgaaagaat ttaaggatat tgctgataac atcaaggagc tcgatttagc
     4741 ggaagatata ccagggataa agcgggagct ggagatgggg cgattgccat atttttttga
     4801 atgtcggcac ggctttaaaa ccttaattga aaatgagccg gagtatctaa tcgctctagc
     4861 gagccgttat gatcaagaac atctgcttat acatagacta tctgatccat acctgcagga
     4921 gttaaagaaa aattatgtag aaaagctcgc tcgtcaaggt gatttggaca ttatttctga
     4981 gttggaaaag aaaggtgagc taagtgcagt tatcagcaaa gaactctatc taagcttggc
     5041 ggatcagcag ggctatgttg ctgaccttga ttgcgctata aatgttgatg atgagatatt
     5101 tgcgcgctat atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnagaaaagc
     5221 aaaagattat cagctttatt atccgcttcg gtgtcgatgc tctaacctat aaaccggaga
     5281 attttcaaag tcttctaaat gaggcaaaag agctgccgtc aagcgtttgg gctgaatcag
     5341 cggagcgttt tcgaaatacc gcagaatttg tgcgcgagat ggaaggagaa gagaagcgga
     5401 gatttctttt ggatctacag attgccgcta gcatattagg caagcaagat acacagtttg
     5461 ttaatttttt cttgaaaaaa gaaggagtta ataccggcgc taatatcgcg agccatgatc
     5521 tgtttgacgc cgcctttagg tatagcgatg gcttcgcctc cttagatgag gcgaaacaga
     5581 aggtgctgag aaagattatt ccagggattg atcgggttgt ttctttacgc gagaaactaa
     5641 aagaatcttc ttcggagacg gaagaaacat taagaaaaaa attggccgac tctttcggat
     5701 ttttaataaa gaactggaaa gatgatgatt tggagaagtt ctgcgggtat gtcaaggagt
     5761 ttgggaacaa gcctttcatt caattattct ccctgtatga acaaatatcg caatgcgaac
     5821 cggcgacgtt accattagag gtgcgagcta gattgctaaa actcaccggt cttaaaatca
     5881 ataagactga aacttcggaa cagctcctgc aaaggttgaa tgagaagatc tctgcgattg
     5941 tgccggaagt ggaccagggc caggctagac agtgtgctat ttccaccttc actaagctga
     6001 gattggacgc attaaacggg aaatatcaag acgctgcttc gggttataca gccccatttc
     6061 ttcgggggga actccgggaa gaggcggcta atggtgatcc tgatcgtttt tcgtcgttta
     6121 attactcatc tctagtagag ggtagggcaa ataatcagtg ggcgatgaat ttgtggctat
     6181 atatttatgg caccgagggc gcattgggag ataaagccgt cgagatggct aagatgtttg
     6241 ataatgccga ataccgcgat gaggttctcc tgaaatataa aaatgaatgg ctggaaaatc
     6301 tttccagtgg agacagccgt ctgaatttga agcttgattc aatttgcagt actgttaatt
     6361 attttggtgg ggccggtaac ctgaaacata tcgagtcatt atctaatctc attactaata
     6421 tccgcgatca tgcctgggga aatcctcata ctgtcgaaag gacgaagaag gagattgaaa
     6481 ctcttctcat cgagcaggaa aagcgttttg ataaggagaa atggagtttg gatgcgcgct
     6541 cggattttta taatctctcc gctgatatca ttgaagcggc ccctagttta tattcttctt
     6601 tccagccttt gtttgatcgt ttggacggca aggagatgaa acaatttgcc cgcgaatgcc
     6661 tgcctctcta tcaagcaaaa ttggttgcta tgcagtcgca agatttgaag tataaaccga
     6721 aagaactcgt gactttgcgc aacgctatca ctgtgttggc ggcaaagttg gaattatacc
     6781 ctgaagacaa gcaagaaaat ctggccgaac ataaacaaga gttgcttgat gatctgcagg
     6841 cgaagtttca gacccgcttc ggtatcaacc atataccgga ggagattaat aaagaaagta
     6901 tccgtacaat tagcaattgc atccgttatc tgggaaatat aagtaggcgt aaccaaaaga
     6961 gtgaagcttt aatttcactc tatctttccc tgagcttgaa taatgagtgg aaggattttc
     7021 gcgctggcaa ggacattgat atcaataaac acttgattcc ggcatgcgca caaaacatcg
     7081 ccgcgatgat agagaaaaaa agagaaaata cgcttccgta tgcgttggtt gatatcgctc
     7141 ccgaagaagt gcctgagttc cagcggatct tgcagtcgga ggtctcctcg acgatgctcg
     7201 gcaacgttca aacgatagac tataagctgc ttaatctcaa gcacaatatt aaagacttag
     7261 ctgatccgga tgcatttcct gaggcgactg ataagcaatt gctggagatc tgtcgcggcg
     7321 gctcaaaatc cttaaatgcc ttgctcgcta aggtctatgg tgaaaccgtc ggtaaaacga
     7381 ccgtctataa tgagtcggag gccaagttaa aagatcaatt ggccaacatc tacggtataa
     7441 agagttggga cgcgactgcg gttaaaagta ttcaggatcg cgtgcggcca atgaacctaa
     7501 ttttcaactt attagataaa atggagaagg aacagcttga tctgcgggta tcggaactgc
     7561 gtgacagtct caagccatct ccggctatta ttgatatcta taaccgattg gaagaagggt
     7621 ttaagccaga atccggcgcc tatgcctcat tccgtgattt agaatattta gaaggactgg
     7681 ccgtgaaggc agaaagccag ttatctgatc aagagaaaaa ggatattgaa tcatatttag
     7741 cacccatcag agagaagatg gctggtttgg aaaggcttat gacgcgagtg aaggagtatt
     7801 ttaacaaatt gtctgatgtt gccaacaaga gtaatcagcc gatgttgaaa gagcgcctca
     7861 aagatattac gaccgctttc gctgacagtg agggcgagtc gattgttcag agttatctaa
     7921 cgagtgattt aaacctcatt attgagaata tgcgccagtg tctcggttgc ttacgcaaag
     7981 aaataaataa cgataccaat ctctcttttg gcgactataa taaattcttc ctgatgagcc
     8041 gccagggcaa agatgaaggc agtatcgcgg atcaaattgt ctttctggca ccagtgaaaa
     8101 atctcaatac cggcgaacag gagctaaact tcattcttga tcgtatttac ggcaataaga
     8161 cttccgatct actcgtcgca cacaccttgg ctgtgcataa aaagtatcgg tctttgaagg
     8221 ataaatttaa aaacgcgaaa ctgggagtaa ttgtttcgaa tatggcgctt ttttccgttg
     8281 gtatcaatca ggatgacttt atcaagaagt tgaaggagaa agctgcgttg gcggcggagc
     8341 cggtatttga agagcggtta gaagcggata ttaaagaatc agctttcagc gatcattatg
     8401 tcgaatttgc cgatcacgaa tatggacgga agaaaggggc actgtctttt agtggcgtca
     8461 tgattaagta ataacttaaa agaaaacaaa aaaggcttcc gttgtggaag tcttttttat
     8521 ttttaaatat aagacaaatt aggcttgaca gatagtgccg agggatttaa gatttaatca
     8581 gataattttg ttatttaaca atttcacttt cacttaaaca catttaccat ggatagacac
     8641 aataaaatcc cagggtctaa aaattctcca gccaatcagg aattgcagca attgttggct
     8701 agtcgcgccc acaagaacat tgtcattcca gatacgaatg ttattgttca ggatcctgat
     8761 tctgttcgag aatttttaaa aggagggaat ctactagtga ttccctggca agtatttctt
     8821 gaattggaca atttgaagaa atccagcgag gtcggctggg aggcaggtaa gggtattaaa
     8881 aatatccatg accttaaaat ggctcaagcg aatttggtta tagaacgccg aacctatttc
     8941 cctagcggca atgatttaaa taaatttgaa ccagatcatc gcattattgc caccgcttta
     9001 tatgtagtca gaaatttaac agttcccggc agccagtatc ggggttatag taaagtcaaa
     9061 ctggtgacca atgattacgg gatgcaaatt gttgcttctg aatttattaa gcgcgatgat
     9121 ttttgctttg aaccatacaa aagagattta atcaaattaa aagaggaatc tttgattatg
     9181 ccggttaaaa agattttgca ggaagaaatc aaaaaaaatg aaaatcggca agagtattgt
     9241 gaaatcggtc aaagggaaaa aattccctac ggaaggccgg ttatgattca tcttaagctg
     9301 ggggagacat ctagtcctta cgctgtcgcc ctgcgtcgag ataatcgcct ggaatttctt
     9361 gatagtaatt tgaaattatc cggcatcggc gcaaaaaaca acggacagcc caactggcag
     9421 cagatagccg ctttgcattt gcttttggac caaagcgttt ccgcagtttt tttgcaagga
     9481 ggcgccggta ccggcaagac actactagct ttagcggctg gcatacacca aagggatcaa
     9541 aaactttttg atcaccttat cattatgaga ccaacgatat atctgagcga agatgataat
     9601 cttggttttc tgcccggcga catcaataat aagttggccc cttggatact ttcaattcgc
     9661 cataatttgg cggaaataaa aaggacccca aaaaacgcca aaattgatga ggatgatcaa
     9721 agtagtttag actttttggt aaaatacggg gttgaaatcc aaccccttgg ttatatcagg
     9781 ggcgccagtt ttaaaagatg ttttatcatt attgaggaag cgcaaaatct tcctcgccat
     9841 ttgattaaaa caattttgac gcggcccgcc gaaggtacca aaattgtctt tactggtgat
     9901 ctcggacaaa tcgacaacaa gcgtttaacc agagaaagct ctggtctcgc ttatgccatt
     9961 aataaaatgt ccgacaacca gatgatcggg attgttaatt ttgaacagac cctaagaagc
    10021 cccttagctt ctttagcgga taagatttta taagatttta taagtaaatt agagccctta
    10081 ttttataagg gctttttaaa tagtcgggaa atcccgccgg cgctgccagt gagggcgggg
    10141 aggagacacg cattgtttat ttgtaaactt atgttataat tttacaaaag atcttatttt
    10201 aagtaatcat tctattttag attcattgtt tttcgattta tgaaaaaatt aattgttttt
    10261 ttatttatat tttgtttttc tttgtttcaa atatcaataa gtttgaaaga aggaattgag
    10321 tctgctcggg ctgatagttt taagaccgtg accgtgactt tcaaggtggc accgacgccc
    10381 gcttctgttg aagttaacaa gaccttagtt aaatataata aagattttat ttttagttgg
    10441 ggttttgatg atggttataa agaggggtat gaatacggct ttaaatattt aaatggtggc
    10501 tataacagta cgctcgattc ttattttggt ggtttatatt acaccgatgg cgccggtagt
    10561 agccgtcctt tttctggttc ttattcctgg ttttcggttg acagttctta taatgatttg
    10621 cattcttctg actctttaag ctatatgagc tgggataatc tgattgattc ttataaccat
    10681 ggctgggtgg ctttaaatca tggttggact tcgatgaatt ttcccgattt agaggcgacc
    10741 tataattacc cggcgccgaa aggaccgggt tcctttgact acaattatga ggttagtcaa
    10801 aatagcagct atattcagga aaaaaccggt ttttatccaa aacattttgt tatccctggg
    10861 ggtgtcagtc atatatccgc ccggcattca atgtcggtat gaagtcggtt tcggctcaag
    10921 ccatcaattt taattatgac ggcaagaatc atctggtgcc ggtcgaaggc gtgcctttga
    10981 gcagtcttaa tcttaatgaa tatttgatgt atcgcaagta ttatgctgag ggaatcaatt
    11041 ttgatctgtc gagtatgacc gaatacgtgg atgccattgc tgccaaaagc accggggcgg
    11101 aaaaatattg ggcagtcggc ttcactcatc gagtttcgcc tgacgcaacc tcaggcaatt
    11161 tatcttggga taaatttaag tatttaatgg attatatagc cgctaattac ggccgtgacg
    11221 gcgctgataa tatttggatg acttcctatc aagaggctta tgaatatgct gaagtttatc
    11281 agggagtttc tctacagacc tctttgtccg gagatacttt gacaatttct tttgacacct
    11341 cgactctccc tggtgatttg cgctattatg ctttaagttt attggtatcc gccgatcagg
    11401 aaattcgcag tgttgattat ggtttaggtg attttaccgc cacttcggct aatttagaca
    11461 gtggccttat aaatcttgat tggggttttt ttcttgatca agatgaatat acacgttcgg
    11521 aagaggaagt ttcgaaggct gaagttagcc ggatatccaa tgatcgcgac aaggctcaat
    11581 cttatgtcaa tgttttaccc gacggtcaaa gaaaaaatat tttgcagaat cgaatagata
    11641 atattgctgt tttaggaaag acttggcgta taaatttcgg tcttaatagc tatatagttg
    11701 gcacttggaa tatttatgga ggcgataaat caccttataa taaattgctc gaaaatttag
    11761 tcgacactga tggcaatgtt tcttctctct ctgtccaggt tgttcaagat tttaccagca
    11821 agtttcttaa taaagggatg aatacgggta ataacagtgg cgttatccct gatgcttatt
    11881 tacaaacggg tttaagcatg tatgcttcaa ctcagcagac aggtatagtc agattcggcg
    11941 gcttggaccc ggccaagaaa tatgatgtat tcatagtcgg cagctataat aatattgctt
    12001 cttccagtaa gagcatttct ttatatacta taaaaggtga aaccaaggaa ttaaatatcg
    12061 cccaaaatac cgctaatggt gttactttcc acgaaatttc tccggagcct aatggttctc
    12121 tggatgtggc tgtttccgct aaagattctt attttggcta cggcgttttg aatgccgttg
    12181 aaatcagaga aagaagccaa aacaagccgt cggctttaat taattccatc actctccgag
    12241 ccaatagtgg cctagttgat ttgaattatt cgttaattca tgatgatgcc gatcctattg
    12301 atttggaata tttacaatat tcgatcactg gtttttttgc cggagaagaa ttaaccgccc
    12361 tttccgcttc cgaccctcaa aataacggca ttagtaatct tgcttcttct atcaccggca
    12421 ctaatcattg ttttgtttgg gatgcccgca ctgatttggg ggagtttgag ggagaggctt
    12481 atttgcgttt tcaacctcga ggaattcacc agtcgggcgc ttttattcaa acagatgcga
    12541 ttaatttgga ttttaaagct ccgattttta gcaatttaac aatttcttct tctgaaaata
    12601 gcgctgagtt ttcttggcac actcatgaag acgcctactc tcgtttagat tacggtcttt
    12661 ctagcgctta cggcttatcg gtcggcagtc ttgatagcgg ttattttttt agagatccag
    12721 actatagtct cggcggtctc catgcctgtg ccgcttatca ttaccgttta ttaggaaggg
    12781 atgctgccaa taatgaaaga caatccgatg atgcttattt tttgacagat ggctgcttgg
    12841 gaggacaaaa gcctgttagt atcttggaat catctttttc ttcgagtgaa tcaaaagatt
    12901 tatccttaga cggcctgcgt ttgcttttgc cctctaatgc tatttctgga agcccgattt
    12961 tacagatttt tcgtttagac gccaacagtg ctatcgcttc cgcaccctta cctgaatctt
    13021 cccatttact tggcgacaag atttttagcc tgaacgctta ttcttcttta agtacgaaac
    13081 agagtcaatt gggcggtagc gccactatta ttattagtta tgatcaggtt gatattttcg
    13141 gattagatcc ctccacttta aggattgctt attggaatgg ctctgcttgg gataatttaa
    13201 gtgattgtag tgtcaatcaa tctgaacgcg aggtttcttg tactaccgat catttttccg
    13261 attttgcctt attgggagat gaaagtattc ccggggaacc gcttttactt tctccaaatg
    13321 gcgccggagt gatagtttct ggttcttcca cccagcagat actttggcag ataccggacg
    13381 atgacgacct aagtcatttt gatttggaat attcttcaga taatggcgct tcttggatgg
    13441 gtttggtttc tggtttggct tctactaccg atacttactc ttggtttgtg ccaccactta
    13501 ataccgccga agctttatta aaagttacag tttatgattt agatttacat agttcctcct
    13561 cgcaatcatc tgtagccttt gtgattgaca ccactaatcc caccgttacc actcctaacc
    13621 taggggctat taatattgcc accgctgctg gtgccaccgc tgccgatacc aatggtatcg
    13681 cctcatattc ttggacgaaa gtttccggtc ccggtactat cactttttct gcctcctcta
    13741 atattttaaa tccaactatc gccgctagca ctgacggctc ctattctgct caactgacag
    13801 tcgttgacaa tgctggcaac accaataaca gtatcataac ttttatttgg gataccactg
    13861 cccctagtat caccattgct cccatttttc ttgaccccac cggtgatacc acgcctattt
    13921 ttaccggtac taccaccgat gctctcatca acatatctgt cgttgaatat caagttgatg
    13981 gcggtccttg gacggcttgc agtgccactg acggcgcttt caattcttta tctgaaaatt
    14041 atacctgcac ggtcgcctcc tctctctccg atggtcttca taccgtctat gttcgtgcta
    14101 gcgataccct gggcaatact tccgacggca gtgtttccga ttcttttaca gttgaccttt
    14161 ctggtgatga aggtgaagtg ctagatggtg gcgatagcga cgaagatgaa gacgataaag
    14221 atgttcaatc agttaccgcc ggcactccta ttaatattag tcctccgcag acgcccgctg
    14281 gcggctttat taagaacggc ttaagtggcc tcaatggcac tttttcatat tttttaaaac
    14341 aaaatgttaa gacatttgat ttagaattac aagccggcga tgatgttgat cgttattctt
    14401 taagcgttga tcctgaattt aaaaatgcta gtattttccc gtatttttct cctttgcgac
    14461 ttgatatttg ctcttttttt gagaattgtc atgatggcat ttatacgtta taccttcgtt
    14521 tttatacccg taacggcatc gcgtcggata atatacgctt ggatatcgat tatcgcggcg
    14581 gggatgaagt taaactggaa gaagaaaatg ataaaaaaga taaaatttca ccgggaattt
    14641 tagctaagcg gttagctgga cgcattcttc ttcaagtaga atctcgaggc gaagcttggt
    14701 atgtaagccc tgaagattat catcgatatt atttacgcga cggtaacgcc gcctatgaat
    14761 tgatgagaaa aatgggccgg ggaataacga ataaaaattt agagcgtttg cggcgagagg
    14821 ctgatttcgc ccgtcgtttt aagggatata tttttcttca ggtagaatct cacggggaag
    14881 cctggtatct tgatgatgat ttaaatcttc attatttacg cgacggtaac gccgcctacg
    14941 aattgatgac cagccttgga ttaggcatca ctaataaaga tttagagcta attccttaat
    15001 ttatgttcgc ctttttttct attttagttt ttcttttttt ggtcatttcc ggtcattatt
    15061 tcgcttggca tctttgccta tcagcctttc cttcttggca ccgctggcga ttctggattt
    15121 tatccgcttt tgtcgccgtc acttttattt tccttggcga ttttatctgg ctgcatcaag
    15181 gtgatagcgc ttttttccga tatttatacg ttttcggcgc gattttattt ggtcttttat
    15241 cgcagctgat gctttttggc tttttatttt atctccatga aatcctgttg aaacactgga
    15301 ccgggaaacg cttgcgttgc gcgtcgcttc aaccccgctt agtcgcccgt ttgcttttaa
    15361 ttctcgccgg cctatttttt attttaggca cctataatgc ttttttgccc agagttaaaa
    15421 cgatagtatt aagaggttgg ccggaggagt tgaaagggaa aagttttgtt cagctttctg
    15481 atttgcactt gggcgcggtc tatcgaccgg cttggctgga gatgattgtt agaaaagtaa
    15541 acgcccttaa tccagatttt gttgtcatta gcggcgattt attcgacggc agcgatgagc
    15601 atttatccga gtttattcct gttttaagaa ctttttctcc ggtaacaatc tttgtgcccg
    15661 gcaaccatga ttattatatc cccgccgaga aagctcaaga aacggcttta gctggcaatt
    15721 tgatatttct tagcgatgcg gccataaacg tggacggttt agaagtgatc ggcctcaatt
    15781 atgtcaatcg agacgatagc ggtttacgga gagaaataaa aaacttgggg gatggccaat
    15841 taacccgaat tgtcgttaat cacgtacccg tggatcaggc ggaagccaaa gccttagagg
    15901 ctcgtttaat gttatcagga cacactcatc gcggccagat atttcctttt tcctttttta
    15961 cctatttact ttacggccgc ttttcctacg gtttagaaaa ctatgaaggg atgacgacct
    16021 ataccaacgc cggcaccggc acctgggaac cgccagtgcg cactcttttg ccgggagaga
    16081 ttatccgttt tgtgattgaa tagtataatt tttataaaaa gaagccgctg gacatggctg
    16141 tcagcggctt tttcttatat tttatggtta tgggtgtcag ccattatttt aattatcccc
    16201 atgtccatta tttgacaatt tttcgtcggg aatgatatag tatatgcgac tgagtcgcaa
    16261 tatatgtctc aaccagaatt aaccaattta ataaattcta ttcgcgctcg tggtcgccgt
    16321 ttatccggcg gacgggaagc tttattgcgt gctctagcga cggcgaataa acctttgtca
    16381 gcggcagcaa ttatgcgttc agtcgccgtg aagcgggcta atttagaccg ggcaacggtc
    16441 tatcggactt taagcttttg ggaaaaagaa ggcgtggtgg aaattttaca tctggaaggg
    16501 gatgagcgct tatatcatct taatctccat caccatcatc atttggtttg tactgtttgt
    16561 aaaaaaatat ctgccgttga tttctgtcaa agtttgagcc gggaagaggc ggcgatagcc
    16621 aagaaaaccg gctttaaaat ccagcgtcac actttagaat tctacggatt atgccgtgaa
    16681 tgtgctcgaa aaaattatta atttttgaat tttaattatc catttatgcc caaacgtttt
    16741 attttcatgg ggatatttgc ccttattatt gctgctttcg cctggacggc ggctgatagc
    16801 tggcagaaga agcagttgtc aaaagacacg cctattcttg ataaggttca agaaacaacg
    16861 gttgccagtg aggcttcacc catcattgtc gtttccagtc tttttccttg gtatgattta
    16921 agccgggaaa tcggcggcga ccgggttaat gctaaattaa ttttgccgcc gggagtggaa
    16981 gcgcacgctt ttgaaccaac gccgggggat atgattagta tcagccaggc tgatttgttt
    17041 gtttataccg gtgattattt ggagccttgg gctcaagaca tcttggccgg tcttaatggc
    17101 agcgctcccg agagcctggc tattggtaaa gatttggcgg agttaccaaa agcagccccg
    17161 gaagaggctg gacttgatcc ccatatttgg cttgatccga tgataatgtt taaggccgcc
    17221 ggccgtttgg cgcagactct ggccaagata gatccggata acaaagccta ttactatgag
    17281 cgcctggaaa attatcaggt gcgtttagag gaacttgatg cgaattatcg ccagaccttg
    17341 tcaacctgta agcagcaaga gtttatttat gccggtcatt acgcttttgg ctatttagcc
    17401 gctcgctatg gtctgcaata tcaagccgct caaggatttt ctccggatgc ggaaagtagt
    17461 ccgcaggcgt tatcagagct gttaaaatcc ctgaaagcgg agtcggctga ttatattttt
    17521 gccgaagagc tggataatcc tcaattggcc aaatctctgg cgaaagacgc cggcgtcaag
    17581 attttaatgc tcaattccgc ccataatgtg agcaaagaag atttaaaggc cggcttgact
    17641 tatgaagaga taatgaaaaa taatcttaaa gaattaagcc ttggactcaa atgccagtaa
    17701 ttgttgaagt taaaaatctt gatattgtct ataattcccg gccagtggtg agggatgtgt
    17761 cttttagtgt ttccgcgggt gattatatcg gcttggtcgg gcctaatggc gccggtaaga
    17821 ccagcctggt taaagctttg tttaatttgc ttcctgttcg tcggggcgag attaaactgt
    17881 tcggtcagag cttaaacacc ttcaagcagt gggagcgttt agcctattta ccccagaagc
    17941 atcagtataa ccgacttttt cccgccagcg tgttagaagt cgttttaagc ggattggcgg
    18001 gtcgtcggcg ccgttatgat aaaacgtcag cgaaaatggc ggccctatct cttttagagg
    18061 agctggcgat ggcggataaa gccgataaat tattcagccg tctgtccggt ggccaacaac
    18121 agcgggtact tttagcgcgc gctttgattc ggaagccgga gctcttaatt ttagatgaac
    18181 caaccacagc cttagacccg atggttagag aaaaattttt tacgctgcta gccaaggcta
    18241 atcaggaaca ggggacggct ataatcatgg tgactcacga tacggccgat atcggccgtt
    18301 atgcccgttt acttctatat cttgatgaag gcttggtttt tgccggtcct tttgccgatt
    18361 ttggtcattc agaaaaaatg aaaaaatatt ttggcgattt cgcccagcct ttatctagcc
    18421 atcagcctga ccattaattt ttatggaatt tttatctttt ttccaatatc cttttattat
    18481 ccgcgcttta attgccggta tcttggtggc gctgccggcg gctcttttag gtttgttttt
    18541 agttttacgc aagctgtctt taatcggcga cggcttgtct catcttagtt ttggggcggt
    18601 agctttaggt ctcctctgcg gtgtctatcc tttttatgtt tccgtgccct tgacagtggc
    18661 cggcgctttt ttgatttttc gtctcggcaa caaggccagg atttacggtg acgccgctat
    18721 cggcatcgtt tctgccgtgg gggtggctgg cggtgtgatt attgccagtt tggctaaagg
    18781 ttttaatgtt gatttattta gctatctttt tggcagtctt ttggcagtca gctgggggga
    18841 gatttatttt ttagctttat tggcggcggc tactcttgtt tttattttca tctattatcg
    18901 ttctttgttt gcctgggcct ttgacgaaaa ttcagctcgc gccgccggtg ttagggtaga
    18961 ttttttaaat aatgttctct tgattcttac agccctaacc gtggcggcgg cagttaaagc
    19021 ggtgggggta atgttggtgt ccgctttgtt aattttaccg gcggcttcag ccttgcaagg
    19081 tgctaaacat tttcgcagcg ctcttatcag tgcttccttt tttgccttta tcgccgttat
    19141 catcggttta gcggcggcca tttactttaa tttgccggcc ggcgccagta ttgtcatcgc
    19201 cggtttcttc ttgtttgtgc ttgcctattt tcgcgctaaa ttgttgaaag tttaaattaa
    19261 ggtttttcca agaaaaaaag accccagact atttgtccag ggttaattgg ggcgaggaga
    19321 gaagcggcta gtttggtaat tttattttga agacataata cgctcaagat tgagctttat
    19381 taggcgctct tgaataacaa aatgattttt atccgccttg gcttttattt tttcccgaat
    19441 atccagccaa tagctttgaa aattggcggc taggactgga tcattaatct tttcaataaa
    19501 atccaagaat tcgttaatgg tgtcatcaac gatttcctta ttgtttgttt tctccaacaa
    19561 acaggcaaaa agatcaaagg cttttttcaa ttctttattt ctatccatgt ttttaaattt
    19621 taaagaacag ttttatttaa atacaaaaat aatcttttgt caaataggag aagaggtctt
    19681 gacttaaaga aaattttgcg catcgattat aatgatttta tgaacaacat tactcttatc
    19741 gctgtcggcg ccttaaaaaa caaggcttta caatctttgg cggccgatta tcaaaaaaga
    19801 ataaaacctt acgcccgttt acagaaactg gaaacgccgg cgtcaccttt tagaaaagat
    19861 gatagaaata aggcaaaaaa gcgggaaaaa gaagctttgg aaaaagtttt gcaccggtat
    19921 ccaaaagagt cgatttttat tttggcggaa actggtcgtc tttttgattc cgtggctttt
    19981 tctaattttt tgagtgagta tgacggtcgc gatttggttt tccttatcgg cggcgctttg
    20041 ggctgggaag aggagttggc gctaaagtat cccgccctct ctttatcacc cttaaccttt
    20101 ccgcatgaac ttgcccgcgt tgtccttttg gaacagatat atcgcggcct tttgcttaaa
    20161 gcgggtaagg aatatcatta ctaggaaaaa tttgatataa taaaaacagg aaataattac
    20221 tttgaatctt aatatttatg atttctcctg caaaaaaaat tattcctgtt tttaataagc
    20281 agcagcgccg cttggtttgg acaatggcgg ttttgaccgt gattgccgct ggtttgctgc
    20341 tggttttttc ttggcgcttg agtcggctta attttttgcc ggcttggtct tctttatcgg
    20401 tcttacccgc tttgttccaa ggagaaacga tgcgagaaac cgcggtcgcc actttaccgg
    20461 ttttttccag cacggaagaa ctatcttttc tcagttcgcc ggacggacgc aatttcgcct
    20521 atatccttaa caataatcag agtcagcagc tggttttaaa tgaaaaagcc ggtccgatcg
    20581 tagatagtgt tacttttatg gctttttctc cggattcccg gagttttgct tatacctata
    20641 agttgaagga caaagagagg gcggtaatta atggccttgt cggcaaggag tatgattgga
    20701 tttttaatcc tcgcttcttt actcccgata gccgttattt tatctataag gctagagaga
    20761 agggcaaaga caggctagtg ataaacagtc aggaaagccg gctttatgat cgtatctatg
    20821 agccctttct gtcaccggat aaagctactt tgattttttt cgctcgcgat ggcgagcgtt
    20881 tgtggcgggg agaaattcct ttagagagaa gcggcgatca atgaaagaaa aaagcctttc
    20941 taagaagaaa ggcttaatat tttttatatc tgcactaggg agattaaatc tcggaggatt
    21001 gttagaatct tcactaagat ttcactcgcc aaggcagaag cttctggagg cattgcccaa
    21061 cgcagaacca tgattagcac caggaagcct atcaaactgt ttaatccttt catatgttta
    21121 aaagattaaa tttgttagag cgggatcctg agtgaccagc tcagggtctg ccactttcct
    21181 tttgagaaag tgcccccttt tttagcgata aatgcttatc ggcaaagaac gggagggttg
    21241 gacttattta aggaactatt ttttactata attatacgga tgaatcctga ttttgttaca
    21301 tttttaaaga tttagcgtta ttattagcca aagcatattt tatgccggtt acatttattc
    21361 aagcagacaa gcgactgaat gcttttttcc gttctgccta taatcgagaa aaggctgaga
    21421 ttttaggtcg tttttttaag accggacccg gtcagtacgg cgaaggggat aaatttatcg
    21481 gcattaatgt cccgactttg cgcgcgctgg ctaaagacta tcaggagatt gatgacgttt
    21541 tcttggaaga actattagct agtccttatc atgaaattag attgctcggc gctttaataa
    21601 tgaccttaag atatgagcgg gcgagcacgg aaaaggacag gctgggcgct ctcaattttt
    21661 atctcaatca tcacttggcg ctcaataatt gggatttggt ggatttgact gtctctaagg
    21721 tctggggcga ctatctttta catcatccgg aaaaaagaaa ggatttatat cgttgggggc
    21781 gaagccagaa tctctgggaa agacgtttgg cggtagtggc cacgctcacg ctcattaagg
    21841 ccggccagtt taaagaaatc cttgatttaa gcgtgatgct tttagatgac caagaggatt
    21901 tgctgcatca agcggtggga tggatgttaa gagaggtggg gaagaaggat aagcaggttt
    21961 tagtcgactt tctcctaaaa tatgccgccc gtttgccgcg gaccactctg cgttacgcga
    22021 ttgaacattt ttcagagacg gaaagaaaga aatatttaaa tttaaaaaga taatttataa
    22081 gctccaaatt aatccgagga ttatccgggg ccaattataa ttatgcctga tattaattta
    22141 tttaaaagtg aagccggtgc caaagcgaag ccggcaatca tcattatcgg tcttgtcctg
    22201 ctagccagca tcatcaccgt tgccttgttg cgcgatcgcc tcgttaatgt ccaatatcgc
    22261 caggtaacgg tgatggggca gggccgggta agctatcatc ccgatttggc ggtgattaat
    22321 ctgggggtgc aaattgacaa ggcgaaacaa gccgaggagg cgttgaagca attgaacgag
    22381 aaagttaata agattatcgc cgccgtccag gcggaaggga ttgaagcggc cgatattagt
    22441 acgcagaact acaatttgta tccgcagtat gattatcaag ataatatttc caccgttgcc
    22501 ggttatagtg ctaatgagca actgagtatc aggattaagg attatgacag tcacccggaa
    22561 agactaaata aagttttggc agcggcggct aaggccggcg ccaaccaaat taacggtttg
    22621 tcttttgatt cctcctccct taacacttta aaacagcagg cgcggatact agctttgcgc
    22681 gatgctcaag ccaaaagcgg ggagctgatg gcggtgaccg gggtaaagat taaaaatatc
    22741 gccggttggt gggagaatgt tatttatcct ccttctgtga cttatgtagg gggtgatttg
    22801 ggcaagggcg gggcggccga ttattctgcc gcgacgccca gtggtaatcg ggaaatcgtg
    22861 attgaggtgg gcgtgaatta taatattaaa taaattgata atagtttagc tggattaata
    22921 agaatccgct tgagtagagg cggattttta ttttgttttt tttcaaagtt gatttttaat
    22981 tttgtcaagc gccagcctca gcttaagccc ttgtatttat tggcacaatt tttaatgtgt
    23041 gctataatat ccctgtaaac aaaggataat aatttttaac tgatatttta tctaataaat
    23101 atcgacttaa aatcatttat ctttgcgttt acagatattt attttttatt aaatatttta
    23161 atcggctcac ttattacgcc gacttgccct aggccggtcg cttcgtagta aaggccccat
    23221 gcaaagcgta tgtccaaaat gactaaggcg cagatcgtcg ccgccatgtc tcaagccacc
    23281 ggcttaagca agaaggacgt taatggtttc tttgaaacct tgtctgaaat gacctacaag
    23341 gaagtcaaga aaaacggaga atgtttagtt cctggtttcg gtaaattggt aaaggcgaag
    23401 agaaaagcgc gcgaaggccg caatcccgcg acgggtgaaa ccattaatat cccggctaag
    23461 accgtcgtca agttccgctt ggccaaggcg gttaaggatg ccgttttata aatggtaatg
    23521 ggtttttata gctatatcaa aaaaagcacc tacgggtgct tttttgtagt aggtagggat
    23581 aaaagcgctt atctatttat atccctggag cttgtcataa cagcttgagg ccaatattgc
    23641 cctaggccga aagcgaatag cttattcttt aattttgtat tccggtgcag atagataagc
    23701 agagaagaga gttctatttg atagcggttc tggctaactc taaatttcaa gttgtcacct
    23761 tcggctttaa gggcgcgacg aatagtttta tcagtgactc cgcccagctt ggcggcttcg
    23821 gagacagaaa gccaaatagg gtttttaaca tggagaccgc gaagatagtt aagcattttc
    23881 ttgattttaa gtctaaatat atttagactt aactttatta tttcttaaat aaaagcctta
    23941 tttataattt tagtctaata tatcatagac taaaattgtc ttatctttct gatattatca
    24001 tttaatcata aaaagtcaag gattatatct aaaggggaaa aataatggct ttaagagcag
    24061 agattgcttg atttcggcgg atattttgtc ttcaccttta ttttttttag cgctaattag
    24121 gttaatttat tagccaaaga tggcttgaaa attagatttt ctggcttgct tttttaaagc
    24181 cttatgttag aatgaaaagg taaaatttgt tttaagcgga gaaagggaag actcccttat
    24241 ttttgattat tatcatgacc ccccatatag tgaaaaaatt tagagagaag cagttattgc
    24301 agcgactaaa aagtcaggac aaggaggcct ttatcaaggt ttatgacgat tatgtccggg
    24361 acatttatcg cttcatctat tttaaagtcg gcaaggaaga agaagcgcag gatttaacct
    24421 caatggtttt tcttaaagcc tggaatcaca ttcagacaaa ttctttaagc gacagcaaga
    24481 ctctgcgcgc tttggtttat aagattgcca gaacttcgat tatcgactat taccgcgaga
    24541 gtggcaacaa gcttgaagtt tctcttaatg acgaagacaa accgatagaa atcatagacg
    24601 agaatcaaga tatggccgct gatttggacc agtcgaccaa tctggagttg attcgtcgcc
    24661 agttgccctt gctcaaagaa gaatatcgcg aagttatcgt tttgcgcttc attaatgatt
    24721 tgaatttgga agaaatagcg gccgttaccg gcaagagccg gggaaatgtt cgggttattt
    24781 tgcatcgagc tttagccgcc ttgaaggcga tggtggaaga taaaatggaa aaaaataaat
    24841 aactgaaata tatttttcct tcacccatct tccttatcta caagtattat attgacaaat
    24901 ctcttttatt tgatattatt aaactggaat tgcttccgaa ctaaattgtg gcgtttaccg
    24961 ccgcgttccc acaagggata ggttcggaag ttttattttg gccaaatttg cacatacttt
    25021 tctttggcgt tttcaaaaac caaaccgcca gcgtatttat aaaaagattc tttatggcgg
    25081 aagccctcgc cccataattt tttaggattt ttgttaatca caattccgta atagaaaaag
    25141 ttatacggag aaactgccac caaaaatgct cgtctaactt tatnnnnnnn nnnnnnnnnn
    25201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25261 nnnnnnnnnn nnnnnnnnnn nnntaataga aaaagttata cggagaaact gccaccaaaa
    25321 atgctcgtct aactttatcg gaattgcgtt tgcaatgaaa ttcatagttt tccggtaggc
    25381 ctaactcctt ttttaatgac tttattcttt ggtcacagtt caacgcttcc tcattatctt
    25441 caaacacaag caaaccgata gtaaaataac ggcttgatcc tttctcaatt tttaatccgg
    25501 aatcaccgga ctcgtcaata aaaactaaca taattttctt gcgttttctt ggtaaatttt
    25561 taattttatg aaaaattcgc accttgtaat actatagcaa ggatggttta aatttaagtt
    25621 aacaaaataa gcgcccccaa acgggtgctt atttgtttat ataaaaaagt tccacctaaa
    25681 tggaacttta tttttttact aaccaccttc ttcctcatca tcattttttt ttacagaaga
    25741 agttagtttt tagaaaaaga aaacaaaaaa atatacaaaa aaaagagaga ataataaaga
    25801 gaaaaatctt taatatgaga atgaatggcg agtgtgggta attaggccaa aatatagaat
    25861 gagctactag ccctgccaat acgaaatagg agatagttac tagatatccg aataaattta
    25921 tccagaaatc ttttattaac aatcttttct ttttcatttt tttaaattta atgaactatt
    25981 gtaataaata tatttagcat attagccaat gcttgtcaag aaggtcgtgt agatacaaat
    26041 aacggttacc gaaacctatc gagtagatac taattcggtt accattataa tcccatataa
    26101 tcccacgggg aattgaaccc tgaagtatcg tgtgttaagt ccgaacttgc atcctcgtta
    26161 gccccacctt gtaacagcat agcagagacc gcttaaattt aagttaacaa aataagcgcc
    26221 cccaaacggg tgcttatttg tttatataaa aaagttccac ctaaatggaa ctttattttt
    26281 ttactaacca ccttcttcct catcatcatt tttttttaca gaagaagtta gtttttagaa
    26341 aaagaaaaca aaaaaatata caaaaaaaag agagaataat aaagagaaaa atctttaata
    26401 tgagaatgaa tggcgagtgt gggtaattag gccaaaatat agaatgagct actagccctg
    26461 ccaatacgaa ataggagata gttactagat atccgaataa atttatccag aaatttttta
    26521 ttaacaatct tttctttttc atttttttaa gttttataat gaactattgt aataaacata
    26581 ttatagtata ttagtcaata tttgtcaagc tgatttgtat ttatagtttt gcgaagatac
    26641 gcgagctcaa cgcaggccgg cgcttaggct tatggctagg attcttccat ctttttagct
    26701 ggggcgattt tatcatataa ctaagctaaa atctaacatc agtctagata ttactggact
    26761 tatctttatt aaaatcaaga aaagtcaata aaaacgggct gatttagatt taaagactgt
    26821 aacaatttta agattattcc gtatattatg gcgtatgagc gataacgatt ttttgaaaca
    26881 attgaagggt ttaaaaaata tctccccggc agcttcttgg cttaaaagca accgggaaat
    26941 tttgttatcc caaatcgcca actccagcgc tcaggaatta acccctcagc gccgtttgct
    27001 gatcgatgtc cgcagctttt tggccgctgt ttctcggccg tctttggtcc tcggttcctt
    27061 tttgttatta ttggcaggcg tcagcttctt cggtcattta gctttcagcc gcactaagcc
    27121 gaatgaatct ttgtatattg ctcgcatcat ttctgagaag gctaaactta atactgtttt
    27181 tgattccgaa gaaagagaca agatggaggt gcagtttgcc actagccatg cggaagctat
    27241 tactgaagtc ttggccggca ccgatgtcaa cgatgaagat caggtggcta aactcaatga
    27301 tagttttaat aaagagttag agacggtgcg caccaagatt gccgtgatta agaaaaataa
    27361 tacgcagact gtcgccgtgc cctcagaaat tccggccgta tccgaagaag atattctttt
    27421 aaccgccggc aacggcaagg atgatgccgg gatgcagatt gctttaaata atagcaccac
    27481 tcctctagcc agcccgaccg ctccggtttt ggcggttgat aaggttacgc cggcagaaac
    27541 cgcgactcca acgccagaag ttgttaccga aaaggaagtg gtggcggata aggcggagga
    27601 agttttaaac gaagctcaga acttgtttga tcataaagaa tatagcggcg cgcttgataa
    27661 gctcaaggaa gtgaaggaga tgattaaata gttaacaatc gtcgccagta tttattagtt
    27721 tgtctctgca gtcgatgcct tttcagaaga tcagattact gctttcttcc gaactggatt
    27781 gcgtgcggag ataaattaat aaatcctgac gacatccgac ggccgcggat ttatctgcca
    27841 ccgccggatt agtccggaga ccagcgagtc tagtcaaggt cgaatcttgc gtgagcaaga
    27901 tgtctcgctc cttataaatt taataatatt tttcgaaaaa atgatttttt cttctcttaa
    27961 tttacatcta tatttatata gaggcaccaa aagagggaat cagagatcag aaaaaacgcc
    28021 cgtagattga ttaagctttt ctccagcctc ggctctcatg ctttagaggc tggcagtcct
    28081 taaatagttt tgtcagtctt taaaaagctt gcttcagccg ttgtgttggc gaggcgggat
    28141 tccgatcaat ttataaatgc ttttctctac ttcaatgatt accaattttc aagccttgaa
    28201 tctaaatagc gataataaag gaaggtggag taaaaaatca acgaaaaata ttttccttat
    28261 catcaacgct ttgttccgac taatatggtc gacgacaagc gcggatgatt ctaattatta
    28321 attatgaaaa aattacgcaa attcttaact gtcggcgtaa tggtattatc aatcatcgct
    28381 atgagcggtt tgactgtgaa cacagctcaa gcggcggctc agcccggtga tttgattaaa
    28441 atggatggct tgtcctccgt ttattacctt ggcaacgacg gtaagagata cgttttcccg
    28501 agcgaatccg tgtacttctc ttggtacaat gatttcagtg gcgtgataac cattcccgct
    28561 tctgaattac agtcctatcc tttaggcgct aatgttacca tgagacccgg caccaagctc
    28621 gtgaagatta ctactgatcc ttccgtttac gccgttactc ctaatggcgt tttacgcaag
    28681 attcagagcg aagctgatgc tatcgccctc tatggtccta actggaacaa gatggtggtt
    28741 gacatcgctg acgctttctt caccaactac accatcggta ctcctttaaa ttccggtgaa
    28801 tatcctgctg gtactttgtt aaagaatccc aacaatgcga gcatctacta ttttgatggt
    28861 actaactatc gcatgatttc cagcgaggct gctttcaatg ctaaccgttt caacatgaat
    28921 aatgttgtta ccaccagcat gactttaacc gcttctggta ccgctatcac tggtgcggaa
    28981 gatttagcta agccttatgg cgctactagc ggttctgttg ttactggttc tggtttgact
    29041 gtagctttag ctgctactac tccggcggcc gcttctgtgc ctgctggcgc tgctattgtt
    29101 ccgttcacta ct
//
LOCUS       DAOQSA010000009        28917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_978_length_28917_cov_9.316922, whole genome shotgun sequence.
ACCESSION   DAOQSA010000009 DAOQSA010000000
VERSION     DAOQSA010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 28917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28917
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_978_length_28917_cov_9.316922"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..872
                     /locus_tag="PKK65_01585"
     CDS             81..872
                     /locus_tag="PKK65_01585"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNU81407.1"
                     /translation="MPPRTIYKMFLVAGLASFILLLSACTTETPNVNDNMDIQDEEVV
                     VNTVSKDKAKIPEKKPELGEFFADTNPSEASTAVAGSQVDLSSSKVQAPDYQPDLLSK
                     YSGAIIKTNQGDITVKFYKNDSPVTVNNFMYLAQAGFYNNSKFHRVIKGFMIQGGDPN
                     SKDDDWSNDGFGGPGYYFKDEINSHKLVAGSLAMANSGAGTGTNGSQFFIVTAEATPW
                     LDGAHTNFGEVVSGMEVVRKIEAAAVNENDHPTTDMVIESISLVK"
     gene            complement(958..1215)
                     /locus_tag="PKK65_01590"
     CDS             complement(958..1215)
                     /locus_tag="PKK65_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNU81408.1"
                     /translation="MKKLFVGGLSFQTTEDVLADAFAKAGTVVSAVIIKDRMTQRSKG
                     FGFVEMETEKEAQAAIAMWNGQELDGRRVAVNEARPLEKRF"
     gene            complement(1416..2132)
                     /gene="rplA"
                     /locus_tag="PKK65_01595"
     CDS             complement(1416..2132)
                     /gene="rplA"
                     /locus_tag="PKK65_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015562943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNU81409.1"
                     /translation="MSKKEEKKVAAPVTKKKAKDFSAIYDHLKAYPLEEAISLVKKLN
                     KTKFDPSLEVHIRLGINPKKSEQQVRGAVSLPHGTGKSVRVAAFVSPANEAACKAAGA
                     EFVGGDELIAEIKKTEKTEFEVAIAEPLMMKNLAAIAKILGTRGLMPSPKNDTVSPTP
                     AKAVEELKKGKISFKNDDTGNLHTVIGKLSFDDKKLAENFQTLMETIRKAKPATAKGS
                     YIKNIAICSSMSPSVKVVIS"
     gene            complement(2197..2622)
                     /gene="rplK"
                     /locus_tag="PKK65_01600"
     CDS             complement(2197..2622)
                     /gene="rplK"
                     /locus_tag="PKK65_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010475526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNU81410.1"
                     /translation="MAKKIKTKIKLQIAGGQANPAPPVGPALGQHGLNIAEFCQKFNE
                     ATRDKMGDIIPVEITVYEDRTYNFILKTPPASELLKKAAGIKKGSGQPRLDKVGQITK
                     AQLAEIAKVKMPDLNARDLDAAMNIIAGTARQMGVEIKD"
     gene            complement(2688..3233)
                     /gene="nusG"
                     /locus_tag="PKK65_01605"
     CDS             complement(2688..3233)
                     /gene="nusG"
                     /locus_tag="PKK65_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNU81411.1"
                     /translation="MAKQLLNQGRRWYVLHTYSGYEENVAQNLKQRVESLDMEDKIFN
                     ILIPTEKKIKIKNGKRKVVEEKIFPGYILVEMEVTDDSWYVVRNTPNVTGFIGTGTIP
                     TPISETEVKALQKRMGVEDPKFTIDVVVGSPVKINEGPFKNMEGKVTSIDEAKGKVKV
                     SVSMFGRETPIELDFLQVKKS"
     gene            complement(3294..3479)
                     /gene="secE"
                     /locus_tag="PKK65_01610"
     CDS             complement(3294..3479)
                     /gene="secE"
                     /locus_tag="PKK65_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNU81412.1"
                     /translation="MSKISRYFQESLEEMKKVTWPTKKETYNYTVLVIVISLAVAAFL
                     GILDYGFNLGLKELIIQ"
     gene            3640..5364
                     /locus_tag="PKK65_01615"
     CDS             3640..5364
                     /locus_tag="PKK65_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81413.1"
                     /translation="MVSPNLVTKKIESSSEELNDNAKKLFQAIYNDLNRKVDDDDESP
                     RLQVSELISKMAFFYEKIRNTVDYKEDHLLRKNAIARILKRQIVIEGLLKMPSTEDLS
                     RHLLSELIQASYLPNNSLPETKVAEVSHILNKYLRLRQECLRAFDSDFGLKTDLNKAK
                     NDIQEKNRLVVWILNLAACEIEENLIRNQTKQAMVNVMFDTLSRILKLPPDLPYGQDL
                     DIQIYLSISRTYSRFDTDMLSFVLFKYYNQGWIGDKIDPMFAPADEKIKEIASNINNL
                     RASIDYQLKHPLQKQLDRLVQRYSLYFTVLQETIDGDPAKVYNEFKQNPNTFISSVRK
                     VCNKKYKKAKSRLWRAALRSIVYIFLTKSIFVFLIEVPAIHWFNEPLNPVALAINISF
                     PAILLFFVVSFTRIPGDDNTDRIVEGVKEIAFVGSERQQPIVLRNPKKRGGVMNFIFN
                     LIYGVAFFVSIYFIIWALTKVNFNWVSITIFLFFLAFVSFFSIIVTRGVKELLVVEKK
                     ENLFSFLLDLFYMPIIMAGKWLSRNVSRINIFIFIFDFIIEAPFKILVDIAEEWTKYV
                     KERKDNMV"
     gene            5367..6068
                     /gene="rsmI"
                     /locus_tag="PKK65_01620"
     CDS             5367..6068
                     /gene="rsmI"
                     /locus_tag="PKK65_01620"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNU81414.1"
                     /translation="MALGTLFVAATPIGNLEDISLRALRILREADYVLCEDTRTTKAL
                     LDKYEVKTPTISYHQHSGPGKTAQLLRYLGEGKNLVLVSDAGTPGISDPGSALVASAL
                     SEYGGKLTVTPLPGPSALISALSISGWPVDRFTFFGFLPHKKGRQTMLKDLLDSSYPS
                     VFYESKHRLAKCLREIIILKAKTGKRVDMILARELTKMFESIYRGSPEELLGLLEKDV
                     DMQKGEFVVLIRKIK"
     gene            6073..7527
                     /gene="metG"
                     /locus_tag="PKK65_01625"
     CDS             6073..7527
                     /gene="metG"
                     /locus_tag="PKK65_01625"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNU81415.1"
                     /translation="MSKENNFYITTTLAYTNSDPHLGFAAELIKADVIARWQSLAGKQ
                     VVFNTGTDEHGLKIFQQAKAAGLSPRAYCDYYAARFASLKKVLDLSFTNFIRTSDDYH
                     EKAAQEFWRRCAENGDIYKKTYSVKYCVGCELEKTESELVDGRCPLHPNRELELIEED
                     NYFFRFSKYQQPLLDFYKNHPDFIIPSHRFTEIKNFVAAGLQDFSVSRLKSKMPNGVP
                     VPGDDEQVMYVWFDALVNYISTLGWPNNEENFQAFWPAVQVCGKDNLRQQTAMWPAML
                     LSAGLPLSRQVLIFGFLTANGQKISKSLGNGVDLFTLVSQYGADALRYYLLTEIPPFE
                     DGDFSEERLIQKYNADLANGLGNLVARVSNLLEKNNIITSLSTDNEAAARYLSKEENL
                     ASYQFSRTLQSIWQDITACNEELSARAPWKLTEREAIAAVLRPLAERILIIATRLQPF
                     LPTTAKRLIAQFTQAQIIKGEVLFPRLDAKSKTV"
     gene            7533..8048
                     /locus_tag="PKK65_01630"
     CDS             7533..8048
                     /locus_tag="PKK65_01630"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNU81416.1"
                     /translation="MSYFDIALIVILVGFVFNGLTKGLIRLLGNIVGLLLGAFIAGHF
                     YLQFYEWGQYLFGGRESLGKVLSFIIVFIAATLIIDWIFVVLEKIFNLISIIPFTKMI
                     NRLLGGALGFLEGSLFIGLILFVISRYAWIGSLFGDYLLSSQVAPFFLTVVNIALPFL
                     PTALKALQSLI"
     gene            8052..8645
                     /locus_tag="PKK65_01635"
     CDS             8052..8645
                     /locus_tag="PKK65_01635"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNU81417.1"
                     /translation="MKIISLPTADNSVFSRDLAAFLKKGKVVMLPTDTVYGFSCRADN
                     AAAIAKIFSLKSREAKKPLLVLVSSLSMVKKFCFINKIQYEKLKKIWSQVRPTSVLLP
                     HRGLLPKSLTAGSPYLAVRLPKSVFLRKIVRASASPLVSTSANLSGQKILAPEEAAKI
                     FSGRKDLDLIVTGGSNKRAVSRLLLLEKNGNLKILRK"
     gene            8651..9877
                     /locus_tag="PKK65_01640"
     CDS             8651..9877
                     /locus_tag="PKK65_01640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNU81418.1"
                     /translation="MDNNYSAGKPTWRWLAIILLMGVSFTAGLFWSNRQTDAVKQLAY
                     SPEALKTDTGSNFNFNLYWEVWDRLKSDYVDKNKIKDEDMFYGSLRGLAASMDDPYTI
                     FMDPKEAQEFSDDLLGTFEGIGAEVGMRNDLITVIAPLAGMPAEKAGIRAGDKIYAIN
                     GESTIGLGVDEAVKKIRGPKGTEVVLTIIRQDEEGPIDIKIIRDQIFVKSVKTEIRKD
                     GVMVITVSNFNDDTLDLFNQAVETALTKNPKGLILDLRNNPGGYLDTAIYMASAWVKE
                     GPVVVEQFGEGKRQEYFATGNNRLGNFKTVVLINGGSASASEIVAGALRDYKKATLVG
                     EQSYGKGSVQSLRNLSDGSALKVTVAKWLTPSGDFINDKGITPDIEVKLSKDDLNKNI
                     DPQMNKALELILKNNK"
     gene            9923..11605
                     /locus_tag="PKK65_01645"
     CDS             9923..11605
                     /locus_tag="PKK65_01645"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNU81419.1"
                     /translation="MKKAKTSRLSSKKEALPPKFIFVVGGVMSGVGKGVSTASIGQIL
                     QARGYKVSAIKMDPYINVDAGTMNPIEHGEVFVTEDGDETDQDLGNYERFLNKNIYRE
                     NYMTTGRIYQTVINRERNLEYKGKCVQVVPHIPMEIRRRIDAALEKSGAEIMIIEIGG
                     TVGEYENLLFLEAARVMKLYNPKDVLFVMVSYLPVPKKIGEMKTKPTQHAVRSLNSAG
                     IQPDFLIARSEQPIDNERKEKLAIHCNVAIADVIAAPDADSIYDIPLNFEKENLGSRI
                     LEKLNLPVRNREPKNEAWKKFVNASKSGKRSVDIGIVGKYFATGNFNLTDSYISVIES
                     VKHAAAANHCKVNLHWLSAEEVEKKGVSVLKGMDGLIIPQGWGGRGAEGKIAAIKYCR
                     EKKIPYFGLCYGMQMAVIEFARNVAGLKDANSAEVNPKTSNPVIHIMPGQEKLIAEKG
                     YGGTIRLGGWPCRLREGTRLSSAYAKYTKDKIVSERHRHRYEFNNDYRTRFEKLGLIV
                     AGTSPDGQIVEAIELKNHPFFVGVQFHPEYISRPLSPHPLFVAFVGACLKEK"
     gene            complement(11678..11950)
                     /gene="rpmA"
                     /locus_tag="PKK65_01650"
     CDS             complement(11678..11950)
                     /gene="rpmA"
                     /locus_tag="PKK65_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNU81420.1"
                     /translation="MAHKKAGGSTALGRDSESKRLGVKLSDGQIAKAGNIIIRQRGTK
                     YHPGENVRRGNDDTLFAVADGKVKFSTKKLKKFNGKLKSTKIVSVK"
     gene            12227..12397
                     /locus_tag="PKK65_01655"
     CDS             12227..12397
                     /locus_tag="PKK65_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010480178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNU81421.1"
                     /translation="MIKVDQEKCIGCGLCAGICPKTFQMNADGKSEPISQETSGCAQN
                     AAASCPVKAISL"
     gene            12400..12876
                     /locus_tag="PKK65_01660"
     CDS             12400..12876
                     /locus_tag="PKK65_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81422.1"
                     /translation="MPGENKIEQTPPSAPDRPLEGNSFENNQEKVLLRPMEKKNEQLP
                     STFPTPTSLASTSLKSGGGDAQSQQLQVIENIMSSGLDQVFLQMNPEEQRRFKEEGEK
                     TANKISLLLNKAKVRVDKIISLIKRWLGLIPHVNKFFLEQEAKIKADNILKIKRNL"
     gene            12880..15417
                     /locus_tag="PKK65_01665"
     CDS             12880..15417
                     /locus_tag="PKK65_01665"
                     /inference="COORDINATES: protein motif:HMM:NF014581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HNU81423.1"
                     /translation="MASFLDNLIIFSKSDSGAVVFSVLFLLVVIALLVGLVFLFRFFV
                     RRFATDNAYFNHQVFLIRLPKEKPQDENKDFSLQQMREEIARGETIFASIGGLKAQRG
                     FTAWLLGRNDHFAFEIVANHHKIAFYAVAPRALSRYLEQQITAHYPEAVIEEIEDYNI
                     FSMSGQVVAASLKTKKHFALPLRTYQKIEVDPLNSLINIMSKLDKDESVAVQYVVRSA
                     NGAWHRKLRSIVKRIHEKNSVREGISGGGVYKVFSFFRDILGAGTGIKNKTKNLNESV
                     SKRLSALEEETVKSIEEKNLRAGLDVNLRIVVSSSSKERANVYLENVASVFTEYNNYS
                     YGNHFSRSLKSGQERQIKDFIYRRFREDYSFLLNTEELTSVFHLPLKHMETPNILWLT
                     AKNAPAPADLPSEGLLLGQNVYRGVKKDIRIKRQDRRRHTYIIGKSGVGKSVLLANMA
                     IQDIQNGEGVCVLDPHGDLIEDILQRMPPERVEDVVIFSPANLERPLALNLLEYDPRY
                     PEQKSFVINEMIGIFDKLYDLKQTGGPMFEQYMRNALLLIMDDPESGSTLMEVPKVLS
                     DENFRRLKLSRCHNRTVIDFWKKEAEKAGGDAALANIVPYITSKLTAFISNDMMRPII
                     GQQKSSFNFRDLMDRQKILLIDLPKGVVGEMNAYLLGMIVVGKILMAALSRTDMPAEA
                     RKDFYLYIDEFQNFTTNSICQILSEARKYALNLIIAHQYIGQLTKNNNTEIKDAVFGN
                     VGTIIAFKIGTEDAEFLVKEFAPVFNVYDLINIDKGTAYIKLLVDNSPSRPFSMKTIW
                     PLLGVKREGISAKIRSLSSLKFGQDRTIVEADIIRRTEALASDLRKV"
     gene            15438..17030
                     /gene="dnaX"
                     /locus_tag="PKK65_01670"
     CDS             15438..17030
                     /gene="dnaX"
                     /locus_tag="PKK65_01670"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNU81424.1"
                     /translation="MPTLYRDYRPQKFADILGQNHLKITLQNELADAKLGQAYLFCGP
                     RAVGKTSMARVLAQAVNCERRKSGESEPCGECAACLSIRRGQSLDVMEIDAASNTGVD
                     NVRENIIASARLAPSGKMRVFIIDEVHMLSLSAFNALLKIIEEPPKQVLFILCTTEVH
                     KVPATIISRCQRFDFKRISPAEIVKKLSFIASQEKIEISREVLEDIARQSGGYLRDAE
                     SLLGQVLALGEKKIGVEEVELILPRHHNQEALDLLDFLAKKNPGGAVSLINQLADNGV
                     NFKNFLEEILLILRKIMLQSVQPGLAEKLGLDFGESLELRISHLSAELPLAACLRILR
                     RFLELSQEKSASIPQLPFEMAVVELCLKAGSDDKSSVNNASNRPTASLVKEASTPKER
                     AVDAPPIIAADLNEAEVAARWPEILIKIKKHNHSLFFVLQNCIPLALKSGLISLRFKY
                     KFHRDRLLDPSIKAIMEETLAEVFQGRVTIEALLDENLELGTDMPVAAGPTDVAAAPI
                     TETKKPGGVLGGLMENFGAEVL"
     gene            17055..18317
                     /gene="serS"
                     /locus_tag="PKK65_01675"
     CDS             17055..18317
                     /gene="serS"
                     /locus_tag="PKK65_01675"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006143131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNU81425.1"
                     /translation="MLDVKYIRENLEEVQKALLKRMEADKLDLESILSLDDKRRDLLQ
                     ASEALKAERNQNSKSKPDDATIAAMKILGEKIKSVEASLREVESRLQASLAALPNIPA
                     DDVLAGGKENNEVIYTFGQQPDFSFPFKDHVSLAESLGIIDYARAAKMSGNGFWCYRG
                     WGALLEWALLSYFIAFHNKNGYQFLIPPFMLTEKSAYTSGHLPKFRDDLFWTQDNLCL
                     NATSEMMLGNYHREEILPLSHLPLRYFAYSPCFRREAGSYRQEERGMVRGHQFNKIEM
                     FIFCEPSDSWKMFDELVKNAQALVEGLGLHYQLSKLAAGDCSAAMAKTYDLEVWLPSM
                     GIYKEVSSISNALDYQARRGQTRYKNKEGENIFVHTLNASGLATSRLLPAILEQNQQL
                     DGSVRIPEVLRPYLPGAPEFIFPDDSKI"
     gene            18323..20326
                     /locus_tag="PKK65_01680"
     CDS             18323..20326
                     /locus_tag="PKK65_01680"
                     /inference="COORDINATES: protein motif:HMM:NF026235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HNU81426.1"
                     /translation="MYLRISLIVLTFLFCWPRLAAASESYPRLANYFLKWEMTETEAK
                     ELARWDLLILDMENQENNPELIRQIRTLNPDVKILAYITSQEIIDNPEDYNNAWLRQE
                     LASKIPSYWYLKDEQGRAVVNWPFTSMLNLSERASKNGEGWKFNELLPKFIHERLQSS
                     GLWDGVFYDNLWGDIAWVNGGNLDFDNDGQRETSLVANSLWAEGVAKMLRFTRELCGS
                     QFIIMGNGRIYWPYQNIINGMMFEGFPSSWENGGTWTGSMESYEKLSRLNVQPQTTVI
                     NVYHKNQEDYQNFRYGFSSALLGNGYYSFDYDVTNHGQTWWYDEYNVNLGSAQSGAYN
                     LLNGGQTDWQAGLWRRDFKNGLTIVNSTDKVQSFSFAKEEFEKIKGTQAPEINNGLKI
                     NFIRLNPKDGVILLKKLAVWRGSGFTNGGFLRIFNSDGAQVRHGFFSYLSSLPSGSEI
                     LVDDFLNDGDNDYIFSSDGKLFRQRHGQNIWSLQPFAPGFKGKISLAAGDINGDGQME
                     IIIGAGKGGGPQVMVFSFEGKVLLNFFAYDKNFRGGVNVAAGDINGDGKDEIITGAGP
                     GGGPHVRYFNQKGEVLGQFFAYDKNFRGGVNVAAGDINGDGKDEIITGAGPGGGPHVR
                     IFNHDGQVISSFFAYSEDFKDGVSVGFSEQSGQPEIAVGIKGF"
     gene            20337..21413
                     /locus_tag="PKK65_01685"
     CDS             20337..21413
                     /locus_tag="PKK65_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81427.1"
                     /translation="MKFSKFISRWWYRRQEKFYRRNRWHLVLDISLVILILLLIGISL
                     RLALYKPGVISSLNTSLHQLNGGQDGRILDIQTEIYQEKNYIEIGNNLEWRIHYRNNG
                     NVKIDNLDFSLDFDSAAFSLKIIKAEDSLNHNLSLDGDKISLKEISIGEEGDLLLQAS
                     WRTEKKDFPRTLKGKLITKAYAGGLSSFKELSWPAINILSDLKLKASVYFHSPQGDQL
                     GIGPVPPIIGVPTKYWLIIKALNQGNDLENLVFSAKLPRGVELGEEQSLLAGKSSYNP
                     DSRLLVWQLDSLAATGGDYIANFVLVLTPTIDQIGQNAPLLTKLQYRAKDLLTGVEIS
                     GSLSDLDSSLPDDPINHGQGKVGE"
     gene            21373..22563
                     /gene="alr"
                     /locus_tag="PKK65_01690"
     CDS             21373..22563
                     /gene="alr"
                     /locus_tag="PKK65_01690"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNU81428.1"
                     /translation="MILLITGREKLENKMINYLRQLLKPKYQTLNRLEIDSEAILYNL
                     NFLQAQQPQAVMIPVLKSNAYGHGLKEICSILNQSSVETIAIDSFPEAQIAYRYFRRR
                     ILLIGEMPIAAYRYLNWSRTEVCVYNGETLKAIASLGVKARIHLFVNSGMNREGIKNL
                     SAFWQEYKDYLSRVKIVGLCSHLLAAEGDGESNRRQEKKFFQDLEFLHQHSCYPVFVH
                     LGNSAGVLALSDNRLNAFRPGLAIYGYNPFTAESPHYQRAQNLRPALRLVTTIVSIQS
                     LSSGETVSYNASYRAERDTKIAVIPFGYYEGLDRRLSNLASFQLLSANKKISVKLVGQ
                     VCMNLSCLDVGNGEEVGVGSEVVLISPNRHDFNSLENLARLQGSTPYELLVKFQANIR
                     KIIV"
     gene            22579..23472
                     /locus_tag="PKK65_01695"
     CDS             22579..23472
                     /locus_tag="PKK65_01695"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNU81429.1"
                     /translation="MMAGKSSPYLARRAKKFNCVKKDYQHKNLHNPFFHKRAEKKATG
                     KKKRKRRFWFFLVIILLSLSLLLYLFFASGIFTLKQIKVSGVSRIAENQLSDAAWQQA
                     QLKRSAIFKQNNLLFFNVDALRETLLNNFSFDSVRVYKQWPQTLVISVGERSLSFIWQ
                     DSQGRSFSDSHGCLIKEVSVRDEDLKTYPVLTATGTKEYLGQNNCLSLEVAYLQAVFT
                     LYDKLKEFPGLNLQEFVLDGAFNTLQIKLNGGPNIFFNIKDDLDKQLKKLSVIKQDKG
                     DEYFKGLEYIDLRYGDRAYFK"
     gene            23484..23624
                     /locus_tag="PKK65_01700"
     CDS             23484..23624
                     /locus_tag="PKK65_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81430.1"
                     /translation="METTFKFLFGFAVFLFSLISIGVFLLILKIILMFTPQIQLLGLT
                     IY"
     gene            complement(23807..24298)
                     /locus_tag="PKK65_01705"
     CDS             complement(23807..24298)
                     /locus_tag="PKK65_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81431.1"
                     /translation="MEQLKESAEISASVNAVLAIVYKHIDYCEDEIDLLVDKRGVYLS
                     LKNINYNPNKPLLTETLTILLKNIVKEFSDAITEVTLVATKKVPRYRRWPWPKKIIGA
                     EFSAAVQSIILRGGIIIKPLNPQAAGKLRSLIVKSYDSDHKEVWINPIITGVVINLTK
                     IQR"
     gene            24399..25355
                     /locus_tag="PKK65_01710"
     CDS             24399..25355
                     /locus_tag="PKK65_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNU81432.1"
                     /translation="MSEQKAITQYLLDFLDYSEIEKGLSSKTQENYTRFLRKFFNWLN
                     SVGLASLKPAALSAEHIWQYKVFLSRHVDPKTKKTLKKSTQNYYLIALRSLLEFFVEK
                     DIASLPPSKVKLAKDRSDKEIKFLKLEQLASLLNSPDTGTIIGLRDKAILETLFSTGL
                     RVAELAALNRDQVRIKNPGQDLEIAVIGKGSKIRTVYFSVRAVNSLKAYLDVRQDFDE
                     ALFINYRRGSARSNHSRRLSVKSVEDIVKKYVKITGLPVMATPHTLRHSFATDLLNQG
                     VDLRTVQEFLGHSNIATTQIYTHVTNKQLRDIHRKVHENRSL"
     gene            complement(25425..26678)
                     /locus_tag="PKK65_01715"
     CDS             complement(25425..26678)
                     /locus_tag="PKK65_01715"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNU81433.1"
                     /translation="MKLKPSKISSFLWAGLALVLLFSGLNIIKKQALGQSDDMVNAEI
                     LQLNQQIQAQKQQLEALAAKQKAYTQAIAAKQAEKNTLSSQLEILEDRSAKAQLEIEA
                     TNLEISKTDLEAKKISLDIMDIDSRIEQQKDHIAKLLRLVYKQDQVSALEILLLNNSI
                     SEFLNQSKYLENANEELRNSLAKLQNDKDRLDKAQQDIQEKSQKLATLKKDLEEKKAQ
                     LAYKQAEKNYLLEETRSSEKAYQDLLKQAKKEAAQTEAEIASLEKTIRDKMSQNDKNR
                     LAGSDSTLSWPVPGRTITATFHDPDYPFRKIIGEHSGIDIRSKQGSPLYAAADGYVAR
                     VKFDGSTAYAYIMIIHNDGLSTIYGHVAGINVVADQYVTKGQLIGKTGGGPGMPGSGP
                     FSTGSHLHFEVRKNGLPVNPLEYLP"
     gene            complement(26733..27716)
                     /locus_tag="PKK65_01720"
     CDS             complement(26733..27716)
                     /locus_tag="PKK65_01720"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HNU81434.1"
                     /translation="MKKINLNLWQISVIVIIAFGAGLIINYAWHSYYQNEKLSTGGDK
                     PNKEVHQSGYKYISPLLECADVHDIRQNDLEKKIKEVINKNIEQGNIIYASLYFRDLN
                     NGPWIGINEQEKFTPASLLKVPLMIAYLKLAEEQEQILNDRLTIENEVTSLSPNILPL
                     KHVKTGQSYTIDDLLKYMIIYSDNAATGALIQHMDPEYLNRIYKDLGINVPNSDQTEN
                     FMNVKEYASFFRILYNASYLNREMSEKSLEILSQSQFDKGLKAGIPANITVSHKFGER
                     IWLENKQLHDCGIIYLEKNPYLLCIMTRGNDFNKMSGVIRDLSATVYENFK"
     gene            complement(27718..>28917)
                     /locus_tag="PKK65_01725"
     CDS             complement(27718..>28917)
                     /locus_tag="PKK65_01725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNU81435.1"
                     /translation="VIMANILGINTGSTNWTDAKSAISDFYNDGRSHLIVTPNPEIIL
                     SAQKDEELFYLLNHADLSLADGFGLKIAAWLSGQKLQRLTGADLLPYLLEEANNKSRR
                     LLIIKPILSLSSETDLLRVLSEKYPHISPLIISTNRQAQAEESDLNKIKNFAPALAIC
                     LLGAPEQEKYLHYLKENMPVLPLAVGLGGAFDFLSGRVRRAPKIMRDFGFEWLWRLIK
                     QPERWRRIWRATFVFLYKLIIWRFVLPYRYRQNVAILMYRLTPEGREIFIVERQGQPG
                     HWQLPQGGLDGLDLVAAGSKEIREESGAIHFKIIASYKKLYRYEFTQENGHYQNKEGE
                     KHFGYRGQEQGLLIVEFTGSPEEIKINYWDHQNWSWVAEKEFIQKLHPCRQEAAFIYL
                     QKLKNLN"
BASE COUNT         7955 a         5346 c         6365 g         9251 t
ORIGIN      
        1 gtttatgatg acgataatat tattaaggtt aggattaaaa gtgtaataag atgacaataa
       61 taaaataaat gatgccaaac atgccaccaa gaacaattta taaaatgttc ttggtggcag
      121 gtttggcatc atttatttta ttattgtccg cttgtaccac tgagactcct aacgttaatg
      181 ataatatgga catccaagac gaagaggttg ttgttaatac agttagtaaa gacaaggcga
      241 agattcccga aaaaaagcct gaattaggag aatttttcgc tgatactaat ccgagcgaag
      301 cgtcaaccgc ggttgcaggc tctcaagtag atttatcttc ttctaaagtg caggcccctg
      361 attatcagcc tgatttatta tctaaatata gtggcgctat cattaagaca aaccagggcg
      421 atatcacggt aaaattctat aagaatgatt ctccggtgac ggtcaataat tttatgtatc
      481 tggctcaggc tggattttat aacaacagca agttccatcg tgttatcaag ggttttatga
      541 ttcaaggagg cgaccctaac agtaaggatg acgattggtc taatgatggt tttggcggcc
      601 ctggctatta ttttaaagat gaaattaatt cccataagtt agtagccggt tctttagcga
      661 tggctaatag cggcgccggt acaggcacca acggctccca attctttatc gttaccgctg
      721 aggctacgcc ttggcttgac ggtgctcata ctaattttgg tgaggttgtt tctggcatgg
      781 aggtggtgcg taagattgaa gctgccgctg ttaatgagaa tgatcatccg accactgata
      841 tggtgataga aagtatttct ctggttaaat aactattatt tgccggcatt taaaaatcgc
      901 ccctgaataa agggcgattt tttgttctag ctatttcaat taaattgatt gttttgctta
      961 gaagcgcttt tccaaggggc gagcctcatt gacagcgacg cggcgaccat ctaattcctg
     1021 gccattccac atagcaatcg cggcttgtgc ttctttctca gtttccatct ccacgaagcc
     1081 aaagcccttg gagcgctgag tcatgcggtc cttaatgata acagcagaaa cgacagtgcc
     1141 agccttggca aaggcgtccg ctaatacatc ttcggtagtc tggaaagaca aaccgccgac
     1201 aaataacttt ttcatgtgtt tgcttttatc tgtttcgccc ggttaaggct taatcagata
     1261 tttgtattta caaattcgta aggcgacctt ttgttgccta taaacagaaa gtggccagtc
     1321 tgtatcgttt cgattgaagc aatacggact agccgctcct gtttctaaca aaacttacgc
     1381 ttgaatttta acttttttta aataaaaagt caagattagg agataacgac tttcacgctg
     1441 ggactcattg aagagcaaat ggcaatattc ttaatatatg agcctttagc ggtggccggt
     1501 ttggccttgc ggatagtttc cattaaggtt tgaaaatttt ccgccaattt cttgtcatca
     1561 aaactgagct taccgataac agtgtggagg ttgccggtat cgtcattttt aaagcttatt
     1621 tttccttttt ttaattcctc taccgctttt gccggagtgg gggagacggt gtcattctta
     1681 ggggagggca ttaggccgcg agtacctaaa attttagcga tagcagccaa atttttcatc
     1741 attaaaggtt cggcaatcgc tacctcgaat tcagtttttt ctgttttctt gatttcagcg
     1801 atgagttcat cgccgccgac aaattccgct ccggccgcct tacaagcagc ttcattggcc
     1861 ggagaaacga aggcggccac tctgacgctt ttgccagtac cgtggggcag actgacggcg
     1921 ccgcgtactt gctgttcgct ttttttcggg ttgatgccca agcgaatatg cacttccaaa
     1981 gaggggtcga atttagtctt atttaatttt tttacgaggc taatcgcttc ttctaaggga
     2041 taggctttaa ggtgatcata aatagcggaa aaatctttag cctttttttt agttacgggc
     2101 gcagccactt ttttttcttc tttttttgac atattttttg tcgtggtgag ttaacgtccg
     2161 gctttaattg ccaggactcc cacaattatt aaataattaa tctttaattt ctacgcccat
     2221 ttgtctggcg gtgccggcaa tgatattcat cgcggcgtcc aaatcgcgag cgttgaggtc
     2281 gggcatctta actttagcga tttcggctaa ttgtgcttta gtgatttgac caactttgtc
     2341 taagcggggc tggccggagc ctttcttgat accggcggct ttttttaata attcggaagc
     2401 gggcggagtt ttaaggatga agttataagt tctatcttca taaacagtaa tttctaccgg
     2461 aatgatatcg cccattttat cgcgggtagc ttcattgaat ttttgacaga actcggcaat
     2521 gtttaagccg tgctggccta aagccggacc gactggtggc gctggattgg cttggccgcc
     2581 ggcaatttgt aatttaattt ttgtttttat ttttttagcc atatctttaa ttcagtcttt
     2641 aaatgattaa gctttagcgc tagaagcggg cgcggcggct tgattaatta agatttttta
     2701 acttggagaa aatcgagttc aataggagtt tcgcgtccga acatggaaac cgaaacttta
     2761 actttaccct tggcttcatc gatgctggta actttgcctt ccatgttttt aaaggggcct
     2821 tcgttgattt taactggaga accgacaacc acatcaattg tgaacttggg gtcttcaacg
     2881 cccatgcgtt tttgtagggc ttttacttca gtttcgctaa tcggagtagg aatggtgccg
     2941 gtaccgataa aaccggtgac attaggcgtg ttgcgcacga cgtaccaaga atcgtcggtt
     3001 acttccattt ccaccaaaat atatccagga aagatctttt cttctacgac cttgcgctta
     3061 ccatttttaa tcttaatttt tttttctgtc ggaatgagaa tattaaaaat tttatcctcc
     3121 atatccagcg attcaactct ctgtttcaga ttctgggcga cattctcttc atatccagaa
     3181 taagtgtgaa gaacgtacca tcgccgtcct tgatttaaaa gttgtttagc cataaattta
     3241 aaatataggc cgagcttttt tgatgtgctc aggcgcttgt ttgtcgaaga aatttattga
     3301 ataataagtt ctttaaggcc aagattgaag ccatagtcca ggatgcctaa aaaggcggcc
     3361 accgccaaag aaatgacgat taccaagaca gtataattat aggtttcttt ttttgttggc
     3421 caagtgactt ttttcatttc ttccagagac tcttggaagt agcggctaat tttgctcata
     3481 ttaaataaaa tttgtaatat taaaaagagc cttcaaggct gctttttgta tctgtttata
     3541 tattagcgaa atcgcttgtt actgtcaaga tcctgaattt ttggacgttg ctttttcggg
     3601 gtaaacaaga taaaataaat agtgaatgaa aaaatctata tggttagccc taatttagtc
     3661 actaaaaaaa ttgaaagcag tagtgaagag ttaaacgata atgccaaaaa actttttcag
     3721 gcgatttata atgatttaaa ccgtaaagtt gatgatgacg acgaatcgcc tcgtttgcag
     3781 gtttctgagt tgatttccaa aatggccttc ttttatgaaa aaataagaaa cactgttgat
     3841 tataaggagg atcatcttct tagaaaaaat gctatcgcgc gtattttaaa gcgccagata
     3901 gtaattgaag gcttattaaa aatgccgagt accgaagact tatctcggca tctattatcc
     3961 gaattaatac aagccagcta tctgcccaac aattctttgc ctgagactaa agtggcggag
     4021 gtgtctcata ttttaaataa atatttacgt ctcagacagg agtgtttaag ggccttcgat
     4081 tcagacttcg gccttaagac tgatttaaat aaggctaaaa atgatatcca ggaaaaaaat
     4141 cgtctggtcg tttggatttt aaatttggct gcttgcgaaa tagaagaaaa tcttatccgc
     4201 aatcaaacca agcaagcgat ggttaacgtt atgtttgaca cgcttagccg tattttgaag
     4261 ttgccgccag atttgcccta tggtcaagat ttagacattc agatctatct cagtattagc
     4321 cgcacttatt ctcgctttga cacggatatg ctttcttttg ttttgtttaa atattacaat
     4381 caaggctgga ttggagataa aatcgacccg atgtttgctc ccgccgatga gaaaattaag
     4441 gagatagcct ctaatattaa taatcttcgg gcgagcattg attatcaatt aaaacatcct
     4501 ttacagaaac aactcgaccg tttagtgcaa cgttactctc tttattttac tgttttgcag
     4561 gaaacaatcg acggtgatcc ggctaaagtt tataatgaat ttaaacagaa ccctaatacc
     4621 tttattagtt ctgtccgtaa agtttgtaat aagaaatata agaaagcgaa aagcaggtta
     4681 tggcgagcgg ctttacgcag tattgtttat atttttttaa ccaaatctat ttttgttttt
     4741 ttaatagaag tcccggccat ccattggttt aacgaacccc taaatccggt ggctctagct
     4801 atcaacatct cttttccggc catacttctt ttctttgttg tttcttttac tcgtattccc
     4861 ggggacgaca acaccgaccg gatagttgag ggtgttaaag aaattgcttt tgtcggctcg
     4921 gaacgtcaac agccaattgt tttgcgtaac cccaagaaaa ggggaggggt aatgaacttt
     4981 atttttaacc ttatttatgg cgttgctttc tttgtctcta tttattttat catctgggca
     5041 ctgacaaagg ttaattttaa ttgggtgagt attactattt tcctattctt tttggcgttt
     5101 gtgagttttt ttagcattat tgtcactcgt ggcgttaagg aattactagt ggtggaaaag
     5161 aaagaaaacc tgttttcctt tttactcgac cttttttaca tgccgattat tatggcgggt
     5221 aaatggcttt ctaggaatgt ttcgcggatt aatattttta tctttatctt tgattttatt
     5281 attgaagctc cctttaaaat tttggtcgac attgcagaag aatggactaa atatgttaaa
     5341 gaaagaaaag ataatatggt ttaattatgg ccttaggcac acttttcgtg gcggcgacgc
     5401 ctattggtaa tttagaggac atcagtctga gagccttgcg cattctgcgt gaggccgatt
     5461 atgttttatg tgaagacact cgcactacca aggctttgct tgataaatat gaagttaaaa
     5521 cgccgacgat ttcctatcat cagcactctg gaccgggcaa gactgcccaa cttctgcgct
     5581 atttgggcga ggggaagaat ttagtattag tatcggatgc tggcactccg ggaatatctg
     5641 accccggttc ggccttagtg gcctccgctt tatctgaata tggcgggaaa ctgacagtta
     5701 cccctctacc cggacccagt gctcttatca gcgctttatc aatatcgggc tggccggttg
     5761 atcgttttac tttcttcggg ttcttgccgc acaaaaaggg cagacaaacg atgttaaaag
     5821 atcttttaga tagctcttat ccctcggttt tctacgaatc aaaacatcgt ctcgctaagt
     5881 gcttgcgaga aattattata cttaaagcta agactggcaa gagggtggac atgattttag
     5941 cccgagagct caccaaaatg tttgaaagta tttatcgtgg ttctcccgaa gaattattag
     6001 gtttactgga aaaagatgtt gatatgcaaa aaggagaatt tgtagtttta atcagaaaaa
     6061 taaaatagat ttatgtctaa agaaaataat ttttatatta ccactacttt agcttatact
     6121 aattccgatc ctcacctcgg ttttgccgct gaattgatta aagctgacgt cattgcccgc
     6181 tggcaaagtc ttgctgggaa acaggtcgtt tttaataccg gcaccgatga acacggcttg
     6241 aaaattttcc aacaagccaa ggcggccggt ttgtctccgc gggcttattg cgactattac
     6301 gccgctcgtt ttgcctcttt aaaaaaagtc ttagatttga gttttactaa ttttattcgc
     6361 acctctgacg attaccatga aaaggcggcc caggaatttt ggcggcgctg cgcggaaaac
     6421 ggcgatatct ataaaaaaac ctatagcgtt aaatattgtg tcggttgtga attggaaaaa
     6481 actgaatcag aattagttga cggtcgctgt ccgttgcatc ccaacaggga attagaactg
     6541 attgaagagg ataattattt ttttagattt tctaaatatc aacagcctct ccttgatttt
     6601 tataaaaatc atccggattt tatcattccc tctcatcgtt ttacggaaat taagaatttt
     6661 gtcgccgccg gtttgcagga tttcagtgtt tctcgcttaa agtcgaagat gccaaacggt
     6721 gtacccgttc ccggagacga tgaacaggta atgtatgtct ggttcgacgc tctcgttaat
     6781 tatatatcga ctttaggctg gcccaataat gaagaaaatt ttcaagcttt ttggccggct
     6841 gttcaggttt gtggaaagga taatttacgc cagcagacgg caatgtggcc ggcgatgctt
     6901 ttatctgccg gtctaccctt gtcgcgtcaa gttttaattt tcggcttttt aaccgctaac
     6961 ggccagaaaa taagcaaaag tttaggcaac ggcgttgacc tctttactct ggtctcccag
     7021 tacggcgctg acgctttacg ctattatctg ttaacggaaa ttcctccttt tgaagacgga
     7081 gatttttccg aagaaagatt aattcaaaaa tataatgccg atttagccaa tggtcttggt
     7141 aatttagtgg cgcgtgtcag taatctttta gaaaaaaata atatcattac ttctttatcg
     7201 actgataatg aagcggctgc gagatattta tcaaaagagg aaaatctcgc ttcttatcaa
     7261 tttagccgca cgcttcagtc aatttggcag gatattaccg cttgcaacga agagcttagc
     7321 gcccgcgctc cttggaagct gacggaaagg gaagcgatag cggcggtttt gcgccctcta
     7381 gctgagagga ttttaattat tgctaccagg ctccagccct ttttgccgac gaccgcaaaa
     7441 cgccttatcg ctcaatttac gcaagcacaa attattaaag gagaggtttt atttccgcgt
     7501 ttagacgcga agagcaaaac cgtttaaaat atatgtctta ttttgacatc gcgcttattg
     7561 ttattttggt tggctttgtt tttaacggct tgaccaaggg cttaattagg cttctaggca
     7621 atattgtcgg tctgcttctc ggagctttta ttgctggtca tttttatctt cagttttatg
     7681 aatggggtca gtatcttttt ggcggccgcg aaagtctagg caaggtgctg tcttttatta
     7741 ttgtttttat cgccgctacc ctgattatcg attggatatt tgtcgttttg gaaaagattt
     7801 ttaatcttat ttccattatt ccttttacga aaatgattaa ccgtttgctc ggcggcgcgc
     7861 tcggtttctt ggagggttct ctttttattg gtttgatttt atttgttatc tctcgttatg
     7921 cctggatagg tagtcttttt ggcgattatt tgctgtcttc ccaagtggca cccttctttc
     7981 ttactgttgt taatattgct ctgccttttt taccgacggc tcttaaggct ctgcaatctt
     8041 tgatttagct tatgaaaata atctccctac cgacggccga taattctgta ttttcgcgcg
     8101 atttggcggc ttttttaaag aaggggaaag tggtaatgtt gcctaccgat actgtttacg
     8161 gattttcttg ccgggctgac aacgcggcgg cgattgccaa aattttttct ttaaaatcaa
     8221 gagaggcgaa aaaaccttta ctcgttttag tctccagttt gtcaatggtt aaaaaatttt
     8281 gttttatcaa taagattcaa tatgaaaaat taaaaaaaat atggtcacag gtgcgtccga
     8341 cttcagtttt gttgcctcac cggggcttat tgcctaaaag tttaacggcc ggtagccctt
     8401 atttggcggt gcgtttgccc aaaagtgttt ttttgcgtaa aatagtaaga gcctctgctt
     8461 ctcccttagt ttcaactagc gccaatctta gcggccaaaa aatattagcg cccgaagagg
     8521 cggctaaaat attttctgga cgtaaagact tggatttaat agtgacggga gggtcgaaca
     8581 agcgggctgt ttcccgttta ttacttttag aaaaaaatgg caatttaaaa atattaagaa
     8641 aataaattat atggacaata attattcagc aggaaaacca acttggcgct ggctggctat
     8701 cattttattg atgggggtct cttttaccgc cggtcttttc tggtctaatc gtcaaactga
     8761 cgcagtcaaa caacttgctt attcccccga agctctgaaa actgacaccg gttctaattt
     8821 caactttaat ctttattggg aggtttggga tcgtttgaaa agtgattatg ttgataagaa
     8881 taaaattaag gatgaagaca tgttttacgg atctttgcgc ggattagccg cttctatgga
     8941 tgatccttac actattttta tggaccctaa agaagctcaa gaattttccg atgatctatt
     9001 gggcactttt gagggcatcg gcgccgaagt aggcatgcgt aatgacctga ttaccgtgat
     9061 tgctcctttg gcgggcatgc cggcagaaaa agccggtatt agagcgggtg ataaaatcta
     9121 tgctattaat ggtgagagca ctatcggctt aggcgtcgat gaggctgtga aaaaaattcg
     9181 cggtcccaaa ggaacagaag tagtactaac aatcattcgt caagacgaag aggggcctat
     9241 agatataaaa attatccgcg atcagatttt tgtaaaaagc gttaaaactg aaatccgtaa
     9301 agacggcgtg atggttatta ccgtcagtaa ttttaatgac gatactctag acctattcaa
     9361 tcaggcggta gaaacggctt taaccaaaaa tcctaagggt ttaattttag atttgcgcaa
     9421 taaccctggc ggttatctgg atacggctat ttatatggct agcgcctggg tgaaagaagg
     9481 gccggtagtg gttgaacaat ttggtgaagg taagcggcag gaatattttg ctaccggcaa
     9541 taaccgtttg ggaaatttta aaaccgtggt cttaatcaat ggcggcagcg cttccgcttc
     9601 ggaaatagtg gccggggctt tgcgggatta taagaaggct actctagtgg gagaacagtc
     9661 ttatggtaaa ggctctgttc aaagtttgcg taatttaagc gatggttcag ctttaaaagt
     9721 caccgtcgcc aaatggctca cgccgagcgg agattttatt aacgataagg gcatcactcc
     9781 tgatattgaa gtgaaattaa gcaaagatga tttaaataag aatattgatc ctcagatgaa
     9841 taaggcttta gaacttatct taaaaaataa taaataaaat taatcaggca ataaggcgtt
     9901 ttaggcgctg ctatattttc ttatgaaaaa agccaaaaca tccagactca gctccaaaaa
     9961 agaggcgttg ccgcctaagt ttatcttcgt agtcggcggc gtgatgtctg gcgtcggcaa
    10021 gggcgtcagc acggcctcta ttggtcaaat tctccaggcg cgcggctata aagtcagcgc
    10081 tattaaaatg gatccctata ttaatgttga tgctggcaca atgaacccca ttgaacacgg
    10141 tgaagttttt gtgaccgaag acggcgatga aaccgatcag gatttgggta attacgagcg
    10201 ctttctaaat aaaaatatct accgggaaaa ttacatgacc accggtcgta tttatcagac
    10261 ggttattaat cgcgagcgta atttggaata taaaggaaag tgtgtgcagg tggtgccgca
    10321 tatcccgatg gaaatccgtc gccgaataga cgcggcttta gaaaaaagcg gcgccgaaat
    10381 aatgattatt gaaattggcg gtacggtcgg agaatatgaa aatttactat ttttagaagc
    10441 tgccagggta atgaaactct ataatcctaa ggatgtttta tttgttatgg tctcttattt
    10501 gccggttccc aaaaaaatcg gggaaatgaa aacgaagccc actcagcatg ctgtcagaag
    10561 tttaaatagc gccggtattc agccagattt tttgattgct cgcagcgaac agccgataga
    10621 taacgagcgt aaggagaaat tggctattca ttgcaatgtt gccattgccg atgttattgc
    10681 cgctcccgac gccgattcta tctatgatat tcctctgaat tttgaaaaag aaaatctcgg
    10741 ttccagaatt ttggagaagc ttaatttgcc ggttcgcaac cgcgaaccta agaatgaagc
    10801 atggaaaaaa tttgtaaacg cttctaagag tggtaagcgg tcggtggata taggaattgt
    10861 tggcaaatat tttgcaactg gcaattttaa tctcacagat tcttatattt ccgtcatcga
    10921 gtcggttaaa cacgccgccg ccgctaatca ctgcaaggtt aatttacact ggttaagcgc
    10981 cgaggaggtt gagaaaaaag gcgtttccgt attaaagggg atggatggtt taattattcc
    11041 tcagggttgg ggagggcgcg gagctgaagg caaaattgcc gctattaaat attgccgaga
    11101 aaagaaaatt ccctattttg gtctttgcta cggcatgcaa atggcggtga ttgaatttgc
    11161 ccgcaatgtt gccggtttga aagatgctaa ttcagctgaa gttaatccga agacgtctaa
    11221 ccctgttatc catattatgc ctggtcagga aaagttaatt gccgagaaag gctatggcgg
    11281 caccattcgc ttaggcggtt ggccgtgtcg tttgcgagaa ggaacacgct tgtcatctgc
    11341 ttatgctaag tatacaaaag acaaaattgt ttctgagcgt catcgccatc gttacgaatt
    11401 taataatgat tatcgcactc gttttgaaaa attaggctta attgtcgccg gtacttctcc
    11461 tgacggccag attgtcgagg cgattgaatt aaaaaatcat ccattttttg tgggcgttca
    11521 atttcatcct gaatacattt ctcgacccct gtccccccat cctttatttg tcgcttttgt
    11581 gggcgcctgt ttaaaagaaa aataaggctc agttacaaaa aataccccgt tgatgaactg
    11641 ggtatttttg tataggtttt tttatctgcg aattaaatta tttaacgctg acaatttttg
    11701 tgcttttgag ttttccgtta aattttttga gttttttagt gctaaattta accttaccat
    11761 cggctacggc gaagagcgta tcgtcgttac cgcgacggac gttttctccc ggatggtatt
    11821 tagttccgcg ttggcggata atgatgttgc cagctttagc aatttggccg tcagatagct
    11881 tgacgcctag gcgtttactt tctgaatcgc ggcctaaggc agttgagccg ccggcttttt
    11941 tatgggccat acaggtttca ggactggcgt cgttttaata aaaagaaaac ggccagccgc
    12001 tttttcataa atatttttaa acaataaagc taatataaca aaatatttta tcattgtcaa
    12061 cttctggtaa attggctttt atcttgataa aaagggaaaa gattagggtt tttaaagctt
    12121 cattttggct gttatttttt taaatatatg ctataatttt aatgttaatt ttaaataaag
    12181 aaaagacggc aggctggagc gtgtcgattt tcctatatta aataatatga ttaaagttga
    12241 tcaagaaaaa tgcattggtt gcggtttatg cgccggaatt tgtccgaaaa catttcagat
    12301 gaacgccgac ggtaaatctg aacctatttc tcaagagact tctggttgtg cccagaacgc
    12361 ggcggccagc tgcccagtta aggctattag tctttaatta tgcctggtga gaataaaatt
    12421 gaacaaactc cgccgagcgc gcccgatcgg ccattagaag gcaattcttt tgaaaataat
    12481 caagagaaag ttttgcttcg accgatggag aaaaaaaatg agcaattacc ttcgactttt
    12541 cccacgccta cgtccttggc gtcaacttcg cttaagtccg gcggtggcga cgcccaatct
    12601 cagcaattac aagtaattga aaatattatg tcatctggct tggatcaggt atttttgcag
    12661 atgaatccgg aagaacagcg acgttttaaa gaggagggag aaaaaactgc caataaaatt
    12721 agtcttttgc taaacaaagc gaaggttagg gtagataaaa taatttcttt aatcaagcgt
    12781 tggctcggtc tcattcccca tgttaataaa ttttttttag agcaagaagc taaaataaaa
    12841 gcggataata ttttaaaaat aaaacgcaac ctttagtcta tggcttcatt tttagataat
    12901 ttaattatat tttctaagag cgatagtggc gctgttgttt tttctgtttt atttttattg
    12961 gtggtcattg ccttgcttgt aggacttgtt tttcttttcc gtttttttgt gcgccgtttt
    13021 gccacggata acgcttattt taatcatcag gtttttttaa tcaggttgcc caaagagaag
    13081 cctcaagatg agaataagga tttttcctta cagcagatgc gtgaggaaat tgccagagga
    13141 gaaacaattt ttgccagtat cggcggctta aaagcccagc gaggctttac tgcttggcta
    13201 cttggccgta atgaccattt tgcctttgaa atcgtggcca atcatcataa aattgctttt
    13261 tatgcggtgg ccccacgggc tttgtcgcgg tatttggaac agcagattac ggctcattat
    13321 ccggaagcgg taattgaaga aattgaagat tataatattt ttagcatgag cggccaggtg
    13381 gtggcggctt ccttaaaaac caagaagcac ttcgctctgc ctctacgcac ttatcagaaa
    13441 atagaggttg atccgcttaa ttctttaata aacatcatgt ctaagttaga taaggatgaa
    13501 agtgttgccg tccaatatgt cgtgcgcagc gccaacggcg cctggcatcg aaagcttagg
    13561 agtatagtta aaaggataca tgaaaaaaat tctgtccgtg aaggtattag cggtggggga
    13621 gtatataaag ttttttcatt ctttagagat attttaggag ctggtactgg tattaaaaat
    13681 aagacaaaaa atttgaatga atccgtgtct aaacgccttt ccgccttaga agaggagacg
    13741 gtaaagtcta ttgaagaaaa aaatttgcgc gccggacttg atgttaatct taggatagtg
    13801 gtttcttcct caagtaaaga acgggctaat gtctatttgg aaaacgttgc cagtgttttt
    13861 actgaatata ataattatag ttatggcaat catttttctc gctcccttaa aagcggacaa
    13921 gaaaggcaaa ttaaggattt tatctatcgt cgtttccgcg aagactattc ttttctctta
    13981 aatactgaag aattaaccag tgtttttcac ctacctttaa agcacatgga aacgcctaat
    14041 attttatggc tgaccgctaa aaatgcaccg gcgccggccg atttgcccag tgagggctta
    14101 cttttaggtc agaatgttta tcgaggcgtc aaaaaagaca tcaggataaa gcgtcaagac
    14161 cgacgccggc atacttacat cattggtaaa tccggtgtcg gtaaatcggt tttattggcc
    14221 aatatggcga tccaggatat tcagaacgga gagggcgttt gtgtccttga tcctcatggc
    14281 gatttaattg aagatatttt acagcgcatg cctccggaaa gagtagagga tgttgtaatt
    14341 ttttctccag ccaacctaga gagacccttg gctttaaatt tattggaata tgatccgcgt
    14401 taccctgagc agaaaagctt tgtgattaat gaaatgattg gaatttttga taaattgtat
    14461 gatttaaaac aaacaggcgg accaatgttt gagcaatata tgcgtaacgc tctcttatta
    14521 atcatggatg acccggagag cggttctact ttaatggaag taccaaaagt tttatcggat
    14581 gaaaatttcc gccgtttaaa attatctcgt tgtcataacc gcacggtgat tgatttttgg
    14641 aaaaaggaag cggaaaaggc ggggggcgac gctgctttgg ccaatatcgt cccctatatc
    14701 acctccaagt tgactgcttt tatttccaac gatatgatgc gaccgattat tggtcagcag
    14761 aagagctctt ttaattttcg cgatttgatg gataggcaaa agattttgtt gattgattta
    14821 cccaaagggg ttgttgggga aatgaatgct tatcttttag ggatgattgt tgtcggtaaa
    14881 attttgatgg cggctttatc tcgtaccgac atgccggccg aggcgcgtaa agatttttat
    14941 ttatatatcg atgagtttca gaattttacc actaattcta tttgtcagat actttcggaa
    15001 gcccgtaaat atgctttgaa tttgattatc gctcaccaat atataggtca gttgactaaa
    15061 aataataata cagaaattaa ggatgccgtg tttggtaatg tcggcacgat aattgccttc
    15121 aaaataggga ccgaagatgc ggagtttttg gtaaaagaat ttgccccggt ttttaatgtc
    15181 tatgatttga ttaatattga caagggcacg gcatatatta aattattggt agacaatagt
    15241 cctagccggc ccttttctat gaaaacaatc tggccgcttt taggagtaaa gcgcgagggt
    15301 atttccgcaa aaattcggtc tttgagcagt ttgaaatttg gtcaagaccg aacaatcgta
    15361 gaggctgata ttattagacg gacagaggct ctggcgtctg atttgcgcaa ggtttaattg
    15421 atattaattt ttaagatatg ccgacacttt atcgtgatta tcgtccccaa aaatttgccg
    15481 atattttagg gcagaatcat ttgaaaatta cccttcagaa tgaattagct gacgcaaagc
    15541 tcggtcaagc ttatcttttt tgcggtccac gggccgtcgg caaaacaagc atggctcgtg
    15601 ttttggctca agcggtaaat tgcgagcgac gaaagtcggg ggagtctgaa ccctgtggag
    15661 agtgcgccgc ctgtcttagt attaggcggg ggcaatcgct ggatgtcatg gaaatagacg
    15721 ctgcttccaa taccggcgtg gataatgttc gtgaaaatat tattgcttct gcccgtttgg
    15781 cacctagcgg gaaaatgcgg gtttttatta tcgacgaagt gcacatgctc tctttgtcgg
    15841 cttttaatgc cttattaaaa attattgaag agccgccgaa gcaggtactc ttcattcttt
    15901 gtaccacgga ggttcataaa gtgccggcga caattatttc tcggtgccag cgttttgatt
    15961 tcaagcgcat cagtccggcg gaaatcgtaa aaaaattaag ttttatcgct tctcaagaaa
    16021 aaatagaaat tagtcgcgaa gttttagaag atattgctcg ccaatcagga gggtatttgc
    16081 gtgatgccga gagtcttttg ggacaagtgt tagctttggg tgaaaaaaag ataggagtag
    16141 aagaggtgga gctaattttg cctcgacatc acaatcagga agccttggat ttgcttgatt
    16201 ttttggctaa aaaaaaccca ggcggggcgg tgtctttaat taatcagttg gctgataatg
    16261 gtgttaattt taagaatttt ttagaggaaa tacttttaat tttaaggaaa atcatgttgc
    16321 aatccgttca gccgggatta gcagaaaaat tgggtttgga ttttggagaa tcactggaat
    16381 tacgcataag ccacttatcg gcggaattac ctttagccgc ttgcttacgc atcctgcgtc
    16441 gttttttgga attatctcaa gaaaaatccg cctctattcc tcagcttcct tttgaaatgg
    16501 ccgtggttga actctgtctt aaggcggggt cggatgataa atcatctgtg aataacgcca
    16561 gcaaccgccc gacggcgtct ttagtcaagg aggcctcaac acccaaggag agggcggtgg
    16621 acgcaccgcc aataatagcg gctgatttga atgaagcgga ggtagcggct cggtggccgg
    16681 aaatattgat taagataaaa aaacataatc attctttatt ttttgtttta cagaattgta
    16741 tacccttagc tttaaaatcc ggtcttatct ctttacgctt taaatataaa tttcatcgtg
    16801 accgccttct cgatccctca attaaggcga taatggagga aaccttagcg gaggtttttc
    16861 agggtagggt gacgattgaa gcgttattgg atgaaaattt agagttgggc acagacatgc
    16921 ctgttgccgc cggaccgacc gatgttgccg ccgcgcccat tacggaaaca aaaaaaccgg
    16981 gcggcgtttt agggggatta atggaaaatt ttggcgcgga agttttataa ttcataattt
    17041 taaaatcagt taatatgctg gatgttaaat atatcagaga gaatttagaa gaagtacaaa
    17101 aggcgctttt aaaaaggatg gaagcggata agcttgattt ggagtcaatt ttaagcctgg
    17161 atgataagcg gcgtgatttg ttgcaggcaa gcgaagcctt aaaggcagag cgtaatcaaa
    17221 attcaaaatc taagcccgat gatgccacga ttgccgctat gaagattttg ggggaaaaga
    17281 tcaaatcagt cgaagcgagt ttgcgggaag tagaatctcg tcttcaggca agtttagccg
    17341 ctttgcctaa tattccggca gatgatgttt tggcgggcgg caaagaaaat aatgaggtta
    17401 tttacacttt tgggcaacaa cctgatttca gttttccttt taaagatcac gtttctttgg
    17461 ctgagagttt aggcattatt gattatgctc gagccgctaa aatgtctggt aacggctttt
    17521 ggtgttaccg cggctggggg gctctattgg aatgggcctt gctgtcatac tttatcgcct
    17581 ttcataacaa aaacggatat caatttttaa ttccgccatt tatgcttacc gaaaaatccg
    17641 cttatacttc tggtcatttg ccaaaattta gagatgattt gttctggact caggataatc
    17701 tttgcttaaa cgcaaccagc gagatgatgc tcggtaatta tcatcgggaa gagattttgc
    17761 ctttaagtca tttaccctta cgttattttg cttattctcc ttgtttccgg cgtgaagccg
    17821 gtagttatcg ccaagaggaa aggggtatgg tccgggggca ccagtttaat aaaattgaaa
    17881 tgtttatttt ctgcgaaccg tcagattctt ggaaaatgtt tgatgaatta gttaaaaacg
    17941 ctcaagcctt agttgagggg ctgggtttgc attatcagtt atcaaagttg gcggcgggtg
    18001 actgcagcgc ggcaatggcc aaaacttatg atttagaagt ctggttgccg agtatgggta
    18061 tatataaaga ggtgagcagt atttccaatg ctttagatta tcaagctaga cgcggtcaga
    18121 ctcgctataa aaacaaggag ggggaaaata tttttgtgca cacccttaat gcttctggtc
    18181 tagccactag tcgtctgttg ccagcaattt tagaacaaaa tcagcaactg gatggctcag
    18241 tgcggattcc agaggttctt cgtccctatt taccgggagc gcctgaattt atttttccgg
    18301 atgattctaa gatttaatat tcatgtattt acgcatttct cttattgttt tgacgtttct
    18361 gttttgttgg cctcgcctgg cggcggcgag cgagtcttat cctcgcctgg ctaattattt
    18421 tctcaagtgg gagatgacgg aaacggaagc caaggaatta gctcgctggg atttgcttat
    18481 tcttgacatg gaaaatcagg aaaataatcc agagttaatt cgccagataa gaactttaaa
    18541 tcctgatgtt aaaatattag cctatattac ttcccaggaa ataattgata atcctgaaga
    18601 ttataataat gcttggttgc gccaggaatt agcttcaaaa attccaagtt attggtacct
    18661 gaaggatgag cagggaaggg cggtggttaa ttggcctttt acttcaatgc ttaatcttag
    18721 cgagcgcgct tccaaaaatg gagagggctg gaaattcaat gaattattgc caaagtttat
    18781 tcatgaacgt ttgcagtcta gcggtctttg ggatggagtt ttttatgaca acctctgggg
    18841 agacatcgcc tgggtcaatg gcggtaatct tgattttgat aatgacggcc aacgcgaaac
    18901 ttctttagtg gccaatagtt tgtgggcgga aggagtggct aaaatgttgc gctttaccag
    18961 agaattgtgc ggcagtcagt ttattataat gggtaatggc cgtatttatt ggccgtatca
    19021 aaatataatc aacggtatga tgtttgaagg ttttccttct tcttgggaaa atggcggcac
    19081 ttggaccggt tcaatggagt cttatgaaaa gcttagccgt ttaaatgtcc agccacaaac
    19141 gacggttata aacgtttatc ataaaaatca agaagattac cagaattttc gttatggatt
    19201 ttccagcgct cttttaggca atggttatta tagttttgat tatgatgtga ctaatcacgg
    19261 ccaaacttgg tggtatgacg aatataatgt gaatcttggt agcgctcagt ctggtgctta
    19321 caatctttta aatggcggcc aaaccgattg gcaagcgggt ctgtggcggc gagattttaa
    19381 aaatgggctg accatcgtta attctacaga caaggtgcaa agtttttctt ttgccaaaga
    19441 ggagtttgaa aaaattaagg ggacgcaggc gccggaaatt aataatggtt tgaagataaa
    19501 ttttataagg cttaatccta aagacggtgt tattctttta aaaaaattag cggtttggcg
    19561 gggaagtggc ttcaccaacg gaggattttt gagaatattt aattcggacg gcgcccaggt
    19621 gcgccatggc ttcttttctt atttaagttc cttgccttcc ggcagtgaaa ttctagttga
    19681 tgatttttta aatgatggcg ataacgacta tatttttagc agtgatggca aacttttccg
    19741 ccagcgccat ggccaaaata tctggtcgct acagcctttc gcgcctggat ttaaggggaa
    19801 gatatcatta gcggctggcg atattaatgg cgatggtcaa atggagatta ttattggcgc
    19861 cggtaaaggc ggtggtcctc aagttatggt tttttctttt gagggtaaag ttttgttaaa
    19921 tttttttgct tatgataaaa attttcgcgg tggcgttaac gtcgcggccg gcgatattaa
    19981 tggcgatggc aaagatgaaa taatcactgg tgccggaccg ggcggtggtc ctcatgttcg
    20041 ttattttaat caaaaaggag aggttctggg tcaatttttt gcttatgata aaaattttcg
    20101 cggtggcgtt aacgtcgcgg ccggcgatat taatggcgat ggcaaagatg aaataatcac
    20161 tggtgccgga ccgggcggtg gtcctcatgt cagaattttt aaccatgatg gccaggtgat
    20221 atctagtttt tttgcttaca gtgaagattt taaagacggc gtgagtgtcg gtttttccga
    20281 gcaatcagga cagccggaaa tagctgtggg cattaagggt ttttaatata aaatatatga
    20341 aattttctaa atttatttcc cgctggtggt atcgccgcca agaaaaattt tatcgccgaa
    20401 atcgctggca tctagtttta gatatttctt tggttatatt aattttgttg cttatcggca
    20461 tctctttgcg tttagctttg tataaaccgg gcgttatcag ttctctaaat actagccttc
    20521 atcaattgaa tggtggccaa gacggacgta ttctcgatat tcaaacagag atttaccaag
    20581 aaaaaaatta tattgaaatc ggtaataatt tagaatggcg tatccattat cgtaataacg
    20641 gcaacgtgaa gattgataat ttggatttta gtttagattt tgacagtgcc gcttttagtt
    20701 taaaaataat aaaggccgaa gatagtctta atcataatct gtctttagac ggcgataaaa
    20761 ttagcctgaa agaaatttct atcggtgaag agggtgattt actgcttcaa gcatcttggc
    20821 gaacagaaaa aaaagatttt cccagaactc taaaaggtaa attaataacc aaggcatatg
    20881 ctggaggtct gtcttctttt aaagaattat cttggcccgc tattaatatc ttaagcgact
    20941 taaaactgaa agcatccgtc tattttcata gccctcaggg cgatcagctg ggcattggtc
    21001 cggtgccgcc aatcatcggg gtgcccacta aatactggtt gattattaaa gctttaaacc
    21061 agggcaatga tttggaaaat ttagttttca gcgctaaatt acccagaggc gtcgaattag
    21121 gtgaggaaca aagtcttttg gccggaaaat caagctataa tcctgattca agacttttgg
    21181 tttggcagtt agattcttta gcggccactg gcggcgatta tattgctaat ttcgttttag
    21241 tgttaacgcc gactatcgat caaatagggc aaaatgctcc actcttgact aaacttcagt
    21301 atcgtgccaa agatttattg acgggtgtag aaatttccgg ttctttatca gatttagatt
    21361 cctctttgcc tgatgatcct attaatcacg ggcagggaaa agttggagaa taagatgatt
    21421 aattatttaa gacaattatt aaagcccaaa tatcaaacgc tgaaccgctt ggagatagat
    21481 tcagaggcaa ttttgtataa tctcaatttt ttgcaggcgc aacagcctca agcggtgatg
    21541 attcctgttc taaaatctaa tgcctatggt catggtttga aagaaatttg ctctattctc
    21601 aatcaatctt cggttgaaac tatagccatc gattctttcc ctgaggcgca gatagcttat
    21661 cgctattttc ggagaagaat tctattaatc ggagagatgc ctattgccgc ttaccgctat
    21721 cttaattggt cgcgaacgga agtttgtgtc tataatggtg agactttaaa ggcaatagct
    21781 tctttagggg ttaaagcacg tatccatctg tttgttaatt ctggcatgaa tcgcgaaggt
    21841 attaaaaatt tatctgcttt ttggcaggaa tataaggatt atctttcccg ggtgaaaatt
    21901 gttggcttgt gttcacactt gttggcggcc gaaggagatg gcgaatctaa tcgccggcaa
    21961 gagaaaaaat tttttcaaga tttagagttt ttacatcagc attcctgcta tccagttttt
    22021 gtacatttag gaaatagcgc tggagtattg gctttatcag ataaccgctt aaacgctttt
    22081 aggccaggtt tggctattta tggctataat ccttttaccg cagagagtcc gcattatcag
    22141 cgtgctcaaa atttacgtcc ggccttgcgt ttagttacta caattgtatc aatccagtcc
    22201 cttagtagcg gagaaaccgt ttcttataac gccagttatc gcgcggaaag agatacaaaa
    22261 attgccgtga ttccttttgg ttattatgag ggccttgatc gccgtttatc taatttagct
    22321 tcatttcagc ttttatccgc taataaaaaa attagcgtta aattagttgg tcaagtctgt
    22381 atgaatttat cttgcttgga tgtgggcaac ggggaagaag tgggtgtggg cagcgaagtt
    22441 gttttaattt ctcccaatcg tcatgatttt aattctttag aaaatttagc gcgcctacag
    22501 ggaagcaccc cctatgaact attggtaaaa tttcaggcta atattagaaa aataatagtt
    22561 tgataataaa tttaatttat gatggcaggg aagtctagtc catatcttgc tcgccgggca
    22621 aagaagttta actgcgtaaa aaaagattat cagcataaaa atttgcataa tccctttttt
    22681 cataagcgcg cagagaaaaa agcaactggc aaaaaaaaga gaaaacgtcg tttttggttt
    22741 tttctggtta tcatcttatt atctttgtcc ctcttgttgt atcttttctt tgcttctggt
    22801 atttttactt taaaacagat aaaagtcagc ggtgttagtc gtattgctga aaatcagctg
    22861 agtgatgccg cctggcagca ggcgcagttg aagcggagtg ccatttttaa acaaaataat
    22921 cttttatttt ttaacgttga cgctttgcgt gaaactttat taaataactt tagctttgat
    22981 tccgttcgtg tttataagca atggccacag acactggtta tcagtgtcgg cgaacgaagt
    23041 ttatccttta tctggcagga tagtcagggg cgtagtttta gcgatagtca cggttgttta
    23101 ataaaagagg tgtcagtaag agatgaagac cttaaaactt atcccgtatt aacggctacc
    23161 ggcaccaagg agtaccttgg tcaaaacaat tgcttgtcac tagaggttgc ttatctccag
    23221 gctgttttta ccttgtatga taaattaaaa gagttcccag gattaaattt acaagaattt
    23281 gtcttggatg gcgcttttaa taccttgcag ataaaactta atggtggccc taatatcttt
    23341 ttcaacatta aagatgattt agataaacag ttgaaaaagt tgtcagttat taaacaagat
    23401 aaaggtgatg aatattttaa aggcttggaa tatatagatt tacgttatgg tgatcgcgct
    23461 tattttaaat aatttcaaaa tatatggaaa caacttttaa atttcttttt ggttttgccg
    23521 tttttttatt ttccctaatt agcatcggcg tgtttttact gattcttaaa ataattttaa
    23581 tgtttacccc ccagatacaa ttattggggt taaccatcta ttaatcagac atatctgctc
    23641 tgaggctatc tttgcaagtc tgtttattta tatgtgtatt aatatacatt atgcgaagta
    23701 tagtaataca gaaaaagctg attaattaag ctttttgtta tacctttctt acttccctta
    23761 ataaaataat ccccggcaac gcataggcat ctgccgggga catttattat ctttgaattt
    23821 tagtgagatt aatcacaacc cccgtgatta ttgggttaat ccaaacttct ttatggtcgc
    23881 tgtcatagct ttttactatc agtgatctta gtttcccggc cgcttgcgga ttaaggggtt
    23941 taataatgat tccgccgcgc aatattatgc tttggacggc ggcgctgaat tcggcaccaa
    24001 ttattttttt aggccaaggc catcgtcgat agcgcggtac ttttttagtg gcaacaaggg
    24061 tgacttcggt tatggcgtca ctaaattctt taacaatatt ttttaacaat attgtcaaag
    24121 tttcagttaa gagcggtttg ttggggttat agtttatatt ttttaatgat aaataaaccc
    24181 ctcttttgtc gactaataag tcgatctcat cttcacaata gtcaatatgt ttataaacta
    24241 ttgccaaaac ggcgttaaca ctggcagata tttctgctga ttctttaagt tgttccatgg
    24301 cgtatcattt tttaaaatta taaagcgctt tattccctct tgtcaatcat cgtatttttt
    24361 tattgctcgc ttgtttcttc atctgctata atatcaatat gtccgaacaa aaagcgataa
    24421 ctcaatatct tcttgacttc cttgattatt cagaaataga gaaagggttg tcttccaaga
    24481 cccaagaaaa ctatactcgt tttttgcgga agttttttaa ctggctaaat agcgtcggtt
    24541 tggcgtcttt aaagccggcg gctttatcag cggaacatat ttggcagtat aaggtttttt
    24601 tatcgcgcca cgtcgatcct aagactaaaa aaactttgaa gaaaagtacg cagaattatt
    24661 atttaatcgc tctgcgctct ttattggaat tttttgtaga aaaagatatt gcttccctgc
    24721 cgccatcaaa agtgaaactg gccaaggata gaagcgataa ggaaataaaa tttttaaagc
    24781 tggaacagct ggccagtcta ttaaattctc cagatactgg cactatcatc ggtttgcggg
    24841 ataaggcgat tttagaaact ctgttttcca ccggattgcg cgtggcggaa ctagcggcct
    24901 tgaatcgtga tcaggtccga attaagaatc ccggccagga tttggaaata gccgttatcg
    24961 gcaaaggcag taaaataagg actgtttatt tttccgtccg cgccgttaac tctttaaaag
    25021 cttatttgga tgttcgccaa gattttgacg aggccttatt tatcaattat cgacgcggca
    25081 gcgccagaag caatcattct cgtcgtttga gcgtcaaaag cgtcgaagat attgttaaaa
    25141 aatatgtcaa aattaccggc ttgccggtga tggcaacgcc tcacaccttg cgtcattctt
    25201 ttgccaccga tctattaaac caaggggttg atttgcgtac ggtgcaggaa tttcttggtc
    25261 atagcaatat tgccaccacg cagatatata ctcacgtaac caataagcag ttgcgcgata
    25321 tccaccggaa agtccacgaa aatcgctctc tttgataaat attttttgtg aacctaaaaa
    25381 acctcggctt gaaaccgagg ttttatggat agttttgtat ttttttaagg taaatattcc
    25441 aggggattaa caggaaggcc gttctttctt acttcaaaat gtaaatgtga gccagtggag
    25501 aaagggccgg aaccgggcat gcctggaccg ccgccagttt tccctatcag ttggcctttg
    25561 gtgacatatt ggtcggccac cacattaatg ccggcgacgt ggccgtatat ggttgataag
    25621 ccgtcattat gaataatcat tatataagca taagcggttg agccgtcaaa tttcactctg
    25681 gcaacatagc cgtcagcggc ggcatagagc gggcttccct gtttggagcg aatatcgatg
    25741 ccggaatgct caccaataat tttacggaaa ggataatcag gatcatgaaa ggtggcggtt
    25801 atagttctgc cgggcacagg ccaggatagg gtgctgtcgc ttccggctag cctatttttg
    25861 tcgttttgcg acattttatc tctaatggtt ttttctaagc tggcgatttc cgcttcggtt
    25921 tgagccgctt cttttttagc ttgcttgagt aagtcttgat aggctttttc tgaagagcga
    25981 gtctcttcca gtaaataatt tttttctgcc tgtttgtagg ctagctgcgc ttttttctct
    26041 tccaagtctt tttttagagt ggccagcttc tggctttttt cttgaatatc ctgttgagcc
    26101 ttatcaagac gatctttgtc gttttgtaat ttagccagac tattgcgtaa ttcttcgttg
    26161 gcattttcca aatattttga ctgattgaga aattctgaaa tagaattatt gagcaataat
    26221 atttctaagg cggatacttg gtcttgttta tagactagtc gcaataattt agcaatatgg
    26281 tctttctgtt gttcaatccg gctgtctata tccattatat caagggagat ttttttagcc
    26341 tccaggtcgg ttttgctgat ttccagattt gtcgcctcaa tttccagctg ggctttagca
    26401 ctacgatctt ctaatatttc cagttgggag cttaaggtat ttttttccgc ttgtttagcg
    26461 gcaatagcct gagtataggc cttctgtttg gcggctagag cttctaattg ttgtttttga
    26521 gcctgaattt gttggtttaa ctgcaggatt tccgcattta ccatatcatc gctttgtcca
    26581 agcgcttgtt ttttaataat attaagtccg gaaaataaca aaacaagagc caagcctgcc
    26641 cataaaaaac tcgatatttt agaaggtttt aatttcatgt tcgtattata acattttttt
    26701 tgatattttg taaaatatat gtattttgcc cactatttaa aattttcata aaccgtggca
    26761 gataagtccc taataactcc gctcatttta ttaaaatcat tacctctggt cattatgcag
    26821 aggagatagg ggtttttttc aaggtagata atgccacaat catgtagctg tttattttcc
    26881 agccagatcc tctctccaaa tttatgcgag acagtaatat tggcgggaat tcccgctttc
    26941 aagcctttat caaattgtga ctggcttaat atctccaagg atttttctga catttcccgg
    27001 ttgagataag aagcattata gaggatgcgg aaaaaagaag catactcttt tacattcata
    27061 aagttttccg tctggtcgct gttgggcacg ttaattccta aatctttata tatcctgttt
    27121 aaatattcgg gatccatatg ttgtatcagc gctccggtgg cggcattatc agagtagata
    27181 atcatgtatt ttagcaggtc gtcgattgta taactttgtc cggttttcac atgtttaagt
    27241 ggcagaatat ttggggaaag actcgtaact tcattttcaa ttgtaagccg atcatttagt
    27301 atttgttctt gttcttccgc tagtttcaaa taggcaatca tgaggggcac ctttagaagg
    27361 ctggctgggg taaatttttc ttgttcatta atacctatcc atggtccatt attcaaatca
    27421 cgaaaataaa ggctggcgta aatgatgtta ccttgttcaa tgtttttatt aattacttct
    27481 ttgatttttt tctctaaatc attttgccta atgtcatgaa catcagcaca ttctaataag
    27541 gggctaatgt atttatagcc actttggtgt acttccttgt ttggcttatc tccccctgtc
    27601 gataatttct cgttctgata ataactatgc caagcatagt taataatcag gccagcgccg
    27661 aaagcgataa tgacgatgac gcttatctgc cataaattca ggttgatttt tttcatttta
    27721 attaaggttt tttaattttt gcaaataaat aaaggccgct tcttgacggc aggggtgtaa
    27781 tttctggata aattcttttt cagcaaccca agaccaattt tgatggtccc agtagtttat
    27841 cttgatttct tcggggctgc cggtaaattc tactattaat aatccttgtt cttgcccgcg
    27901 gtagccgaaa tgcttctctc cttctttatt ttgatagtgt ccgttttctt gggtaaattc
    27961 atagcggtat aattttttgt aactggcaat tattttaaaa tgaattgcac cactttcctc
    28021 tcttatttcc ttactcccgg ccgctactaa atccagtcca tccaaaccgc cttggggcag
    28081 ttgccagtgc ccgggctgtc cttggcgttc aacaataaaa atttctctac cctctggcgt
    28141 gagtcgatac attaaaatag cgacattttg ccgatagcgg taaggaagga cgaagcgcca
    28201 gatgattaat ttatatagaa atacaaaagt ggcccgccag atgcgccgcc agcgttcagg
    28261 ctgttttatt aggcgccaga gccattcaaa gccgaagtcg cgcattatct ttggcgctcg
    28321 tctgacgcgt ccgcttaaaa agtcaaaagc gccgcctaat ccaaccgcca aaggtagaac
    28381 cggcatgttc tcttttaagt aatgaagata tttttcttgt tccggcgctc ccagcagaca
    28441 gatggctaat gccggtgcga agtttttgat tttatttaag tccgattctt ctgcttgagc
    28501 ttgacgattg gtgctgataa tcaaagggga aatatgagga tacttttccg aaagaacacg
    28561 aagtaagtcc gtctcgctgg agaggctgag aataggcttg attatgagga ggcgtctgga
    28621 tttattgttg gcttcttcaa ggagataggg cagcaagtcc gccccggtga gacgttgtaa
    28681 tttttgccca ctgagccagg cggcgatttt aaggccaaag ccatcagcca aagataggtc
    28741 ggcgtgatta agtagataga agagctcttc gtctttttgg gcggatagga taatttctgg
    28801 attaggggtg acaatcagat gggaacggcc gtcattataa aaatcactga tagctgattt
    28861 tgcgtcagtc caatttgttg acccggtatt gatgccgagg atattagcca taatcac
//
LOCUS       DAOQSA010000010        28658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_996_length_28658_cov_8.733000, whole genome shotgun sequence.
ACCESSION   DAOQSA010000010 DAOQSA010000000
VERSION     DAOQSA010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 28658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28658
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_996_length_28658_cov_8.733000"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..1548
                     /gene="dnaA"
                     /locus_tag="PKK65_01730"
     CDS             172..1548
                     /gene="dnaA"
                     /locus_tag="PKK65_01730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNU81436.1"
                     /translation="MNNDQIWQAVLGELEINLSRVNFITWFKDTFLSSFENGRAIICV
                     PNAFVKKWLEEKYHRNIVSSLENITKQKLNEIIYKIELRHGKQENVITLENVAIPKSQ
                     DKPKENIVSQNVDRFGLNPKYVFDNFVVGRGNELAHAACRAVVNNLGKAYNPLFIYGG
                     VGLGKTHLLQAVGNEAAKKTDKILYTTSEKFTNNYIQAVQTGKAKDFKNMYRNVDVLL
                     VDDVQFMGGKDGTQEEFFHTFNELQQSDKQIVMTSDRPPKSIPAIEKRLISRFESGMV
                     ADVGKPDIETKIAILEQKSLEKNFPLDKDVLMYIANNVQNNIRELEGALNKIIVIHQL
                     NNSIPTLKSVKNALSDYVSSLQVKSLTPKEIIEVVSRFYEISVKDIIGNSRRKELVGP
                     RQVAIFLIREELNTSYPSIGQEMGGRDHTTAMHAYNKILNEIKENDNEKLRQEIESIK
                     QLFNNSAY"
     gene            1808..2935
                     /gene="dnaN"
                     /locus_tag="PKK65_01735"
     CDS             1808..2935
                     /gene="dnaN"
                     /locus_tag="PKK65_01735"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNU81437.1"
                     /translation="MKFICLQENLKKGLAIVNHINNKNINLPILNNILIRANDNDNSI
                     ELISTNLEIGITHQLRGKIEEGGETTIDAKIISDYVNLLPEEKIEFKEDNNEINIKCE
                     NYKTKVKGESSKEYPLIPVINRNNFCEINIFDFRKALNSVVFAVANNDNRIELSGVFF
                     QFQSDVLTIAATDSYRLAEKKIKIKNNSINETNIIVPARTIQEILRIISNFSSDAQSL
                     ESDIIKIYLSDNQILFSLDSVELISRLINGQYPDYQQIIPDKYKTEVEIKRQDLIRAI
                     KASAIFSKSGIYDISLIFKKGVVNVYASSGQSGESSIDLNGDVSGDDNDITINCRYLL
                     DGLNIIDSEQVIIKLINNNTPCAVVSRDDDDYLYIVMPIRN"
     gene            complement(3031..5544)
                     /locus_tag="PKK65_01740"
     CDS             complement(3031..5544)
                     /locus_tag="PKK65_01740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNU81438.1"
                     /translation="MPIPKLKTVNAPKAEFWRKGQTPKKKKIKIKHKIIIALILLASL
                     GLLSVFIIVAWVSRDLPNPNQLIDREIAQSTKIYDRSGEHLLYEIHGEEKRTLISLDD
                     IPENVKHATIAIEDKNFYKHGGFSLWAMFRTAITNVIYNRKAGGSTLTQQFIKNAILS
                     SEKSYIRKIKELVLAQRIEKKFSKDEILQMYLNEIPYGSNAYGVEAASQKFFGKSVRD
                     INLAESALLAALPQAPSRYSPYGPNVDILMGRKDYILDLMAEQGYISNAEKDAAKSYQ
                     IVFQRAETSITAPHFVMMVKDILSEKYGENEVERGGLKIYTTIDWNKQKIAEEVIKEK
                     TENYEDKYNANNAALVAIDPKTGQVLSLVGSRDYFNDEIDGQVNVADRPRQPGSSMKP
                     IVYAALFERGYTPNTKLYDVITNFSTDNGAPYTPHNYNGKEYGPVSIRQALSGSLNIP
                     AVKAIYLAGIDKVLNLADNLGYTTLTDRSRYGLSLVLGGGEVKMIEHVNAYSVFAREG
                     KINKLSLILKIEDKDGKVLEEYKNQESRVITTKTAQMINSILTDNEARAFIFGAKNWL
                     TLNDRVVAAKTGTTNDYKDAWTIGYTPSLVAGVWVGNTDNSKMNSGADGSVVAAPIWN
                     EFMRRALTGTPVESFNPWQEEATGKAAIDGTIQFSKKIIINKETKLPANSDTPDELKE
                     EIEIFNHHTILYYVNKEDPLGPEPENPTEDPQFNSWEEAVKRWAKDNNQLSSEDLINQ
                     AELIKPENKPTIIIESPSEGELVNKKILKVKVNASAPRGIKSIKYQLNGSALPGLGAE
                     TEIDLFLDGLEKGYQKITATACDDVGNCNNAEINFNLVY"
     gene            complement(5546..6457)
                     /locus_tag="PKK65_01745"
     CDS             complement(5546..6457)
                     /locus_tag="PKK65_01745"
                     /inference="COORDINATES: protein motif:HMM:NF013269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4931 domain-containing protein"
                     /protein_id="HNU81439.1"
                     /translation="MKNQAQIRYNPLNGRSIIFAPKRAKRPNDLKNKTKESSCPFCSA
                     NVKKTNILQQWPSSGKWQSAAIKNIYPALSNKNKNAYGYQEIIIDSGHHYENFLELNL
                     KQISSLLKLFQLRNQALSKDKKIKYILTFKNQGRAAGASLRHIHSQIFASSILPPEII
                     EERNRTARYQAENKVCPYCLLINNEQKSQRLVWQDSKMIAICPFASEFPYEVWIISKR
                     HVDNISLLNNKELKSLAQALKLIAKKLKSLEYDFNFFAHNDIDNQHQHFYIKIQPRPN
                     IWAGIELGSGLIINPVMPEEAATYYRK"
     gene            6911..7693
                     /locus_tag="PKK65_01750"
     CDS             6911..7693
                     /locus_tag="PKK65_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HNU81440.1"
                     /translation="MDTTITSLQNPLIKNIVKLRKVRSRQKQKIVVVDGLREIKVAFN
                     YGFEFIEILYCPELSQETWTQAPLRPIIVSKEVFFKIAYADNPNGWLALVRPRYRSLS
                     DLKIKTNPLILVLEAVEKPGNLGAMLRTAYAAKVEAVILNNLQTDLYNPNVIKASSGY
                     VFSDQVVLASQEDTLDWLKSKNIKIFATNIKAAKKYTDVDWRQAAALIMGTEATGLSD
                     FWINNADNNIIIPMQAGIDSLNVSVSAGILIFEARRQRDLGK"
     gene            7769..7840
                     /locus_tag="PKK65_01755"
     tRNA            7769..7840
                     /locus_tag="PKK65_01755"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7802..7804,aa:Arg,seq:tct)
     gene            7878..8048
                     /locus_tag="PKK65_01760"
     CDS             7878..8048
                     /locus_tag="PKK65_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81441.1"
                     /translation="MENKKKAALVKKITKAKTIKKEPVAKKKTREEIEKMFAFNFGCD
                     CSCCSHRCDSYK"
     gene            8071..8141
                     /locus_tag="PKK65_01765"
     tRNA            8071..8141
                     /locus_tag="PKK65_01765"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8103..8105,aa:His,seq:gtg)
     gene            8523..9134
                     /locus_tag="PKK65_01770"
     CDS             8523..9134
                     /locus_tag="PKK65_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81442.1"
                     /translation="MKSKKNLLEQLKTFPHFNKNTVHQLGRQWELKDSTINTYISRFL
                     KYKDVLPLKNGLYVTTDFFKNNRSDVSYSFYLANIIRTPSYVSSWAALQYYNLATEAV
                     HSITSTTLKVTREYQTKAGNFAYQSIKKDFFSDFFLVKGKFDFYIASPAKALFDLLYF
                     RTRQLKGINLEDIKKMIEELRIDFDEMDKTEQEKFYSIIKKLI"
     gene            9248..10426
                     /locus_tag="PKK65_01775"
     CDS             9248..10426
                     /locus_tag="PKK65_01775"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNU81443.1"
                     /translation="MNFICHHPEYSKWIMYGGSALRIIHGLDRMSVDLDFEVPHAITE
                     KFLEELKKEVEGYFVNTYGADSDFLTIKITTGRGLLLKFHVGKELSLGNPSNQVHVKI
                     DLNHFVAPKTVTERRPINRDQLSFVILTYNMRALMASKLAAIFLRGPRGVGDVVYEEK
                     GRDIYDLLWYMGKKIVPDFDYMTAKGIDVKDPRTLFDKLTLQMNKVSDKNLKNDLTPL
                     FVDQKFISNWLKNWRESYFRLLNEYKIHTIKNLESVGISRDFNMDVFSDVFSFIYWFN
                     TEEESSVRVVYRISDYWINFRDGDLAIDVDKKLEDKMEFNGNGWSSRPTFQDKLKRYA
                     TLFYQKTEKYFKKTNHIMLGDVIITKVIRMTADNLNQKEQIVLNKSALLSCELDDLLK
                     "
     gene            complement(10580..11242)
                     /locus_tag="PKK65_01780"
     CDS             complement(10580..11242)
                     /locus_tag="PKK65_01780"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNU81444.1"
                     /translation="MKMLIKNLISVMMLLIFWVLGWWLFKIKNKTTIIGRENIPKKTS
                     LLFLSNHQSLIDSMLIGYALFSPWDIFRHYRQIPWNAAAWENFFKKPIRRIFCYFLKT
                     IPAYRQSNIQTANRNIKEFKNILENGNLLLFFEGTRSRNGEIGACKYGPAKLVVDLQP
                     VTIPIRISGMDKVMPINIGFKWLRVKGGQKILIKIGPAIKFDSMEINDVRQKIQNEIK
                     KL"
     gene            11295..11486
                     /locus_tag="PKK65_01785"
     CDS             11295..11486
                     /locus_tag="PKK65_01785"
                     /inference="COORDINATES: protein motif:HMM:NF019522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HNU81445.1"
                     /translation="MANCVLFSFKHEPFLSFSQVINNEEMATEIVRSLVGAIGLCLTM
                     PIATGLSVWLLFKEKKSLN"
     gene            11559..12281
                     /locus_tag="PKK65_01790"
     CDS             11559..12281
                     /locus_tag="PKK65_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81446.1"
                     /translation="MQSVFKWKKIFFSLLALNFVLSAFVPFLIKSDFLGLSEEAVEVL
                     IIALLLILAYVVNFLYLREVGRLKRYQVNLEDRLQDTFKYIGSVNLQIEEMRQAFANF
                     KKYPESKKDIRTVFIYFSEKILSIVNADWVILRIIDIKTGRTIRDGTFTRNNQKINHF
                     KFDNQEILSGQCRSEQCTIVKSDQENLNIKACCILPSKLNGRDQEFFVRSMVNQLEMM
                     FIVFSSLSNRKVGNSKNRIKKK"
     gene            complement(12333..13700)
                     /locus_tag="PKK65_01795"
     CDS             complement(12333..13700)
                     /locus_tag="PKK65_01795"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNU81447.1"
                     /translation="MFNPEIFKAYDIRGVFGKDFDINFAYNLGRAFVSLRQLDGDCPL
                     NQPIKILVARDMRLSSPALNEGLISGLTSAGADVIDAGLISTPAFYFNVSKQNADGGI
                     MISASHNPKEWNGFKMVRTKSRPVSSDSGINWLKDNIGQIAMPPGKATARQLTDAAKS
                     QLEHDLKFIDIPAIKPLRIIADAANGMGAQYLDLLFEKLPINLERLNFQLDGTFPAHE
                     ADPLKEENLNELKEAIINKEADLGIATDGDGDRVFFVDNHGELIDPAIIRGLLAQIFL
                     KDKPGSKICYDVRPGKITVDLIEEAGGIPVPTRVGHSLIKEKMLAENAYFAGESSGHF
                     FLNLAIGCFEMPMIIIGQMLTLFSQSDLTTSDFIRPYRRYFHSGEINRHVNNKEAIFS
                     AIQKKYADGNISLLDGVSVTYPDFWFNVRGSNTEDKVRLNLEAKEKEVMEKKRDEILQ
                     LIETC"
     gene            complement(13693..14820)
                     /locus_tag="PKK65_01800"
     CDS             complement(13693..14820)
                     /locus_tag="PKK65_01800"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNU81448.1"
                     /translation="MLKNIKLSLIIGGLLILSPLLVSAADIKSGDNVYLPPQETIEGN
                     LYAASNNITIDGEIKGDLIAAAQTIIVNGRLDGDLIAAAQTIIVNGEVNGNVRLAGNS
                     AQINGTVARNLNFIGNALDINKNAKIGWDLMSGANNINIAGLINGNVYGNGNNIVLSG
                     VVGKNLEFIGNKNSGISLSSEANIGGDLNYSENIKILKKDGAQISGQTKLIKNKEKTK
                     NKYYFFSIIYKILAAIIIGLIIIFPGKKIINALHDTAQKNPWASLGWGMLILLATPTL
                     SLILIFTIIGIPLAILGFLVWFLVLYLGKIISAIFIGKAVNKILNKKKEKSLLIPLLA
                     GVTIAWLLFSIPFIGWIISLSATCLGLGSLIMYLKTKKRYV"
     gene            14873..16453
                     /locus_tag="PKK65_01805"
     CDS             14873..16453
                     /locus_tag="PKK65_01805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HNU81449.1"
                     /translation="MIEAIRELIERAQISVKNRDNLLSLKREISKKYKIDSLLNADII
                     KEYKRLLSLKLIDSNLILENLLRKRTIRTLSGIAPVAVLTKPYPCPGTCAYCPRQKKV
                     PVSYLSNEPAVMRAIRCGYHPYTQTASRLRALENNGHQPEKIELIVIGGTWSYLPVKY
                     KYWYVLNCFRAANDYLSFGELKKSPYRATLHTAELKRLLLWEQKKNEKARYRIIGLTL
                     ETRPDYINTKELEEMRLLGCTRVEMGVQITDDKILTLNRRGHGVKEIAEATALLKKYG
                     FKITYHIMPALPGSNPKLDFQLFSGLFKDERFQPDQIKFYPTVVTAGSLLYKWYQQGK
                     YKPYSDKVLQKLIIKCKAVVPEYVRIIRLIRDIPSESIIAGNKITNLRQIMKQQGVYC
                     RCIRCREAKDAPISSYSINIQKYQAAGGWEYFISADSLDKSILFGFCRLRLDKSSPIA
                     PAIVRELHVYGELTPFGTGFKVQHRGLGKELLVKAEQLALTANSDKIAVISGVGVRDY
                     YRRRGYRLYNTYMMKKLN"
     gene            complement(16500..16670)
                     /locus_tag="PKK65_01810"
     CDS             complement(16500..16670)
                     /locus_tag="PKK65_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81450.1"
                     /translation="MSHEIEANFVYGWENENSQCQRCNSFQIMEDGKGFCTEAQAEVP
                     ADAHCDFFQSID"
     assembly_gap    16742..16841
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17250..17867)
                     /locus_tag="PKK65_01815"
     CDS             complement(17250..17867)
                     /locus_tag="PKK65_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81451.1"
                     /translation="MEKMSMPKLEKNITAKEDSFYSVSELFQSDEANQELSSSKIEEL
                     KVEDNKELEKIRLQIENLKPEDNKDIGKNHLQIDKEKIEPSRNNNIKNPSPNLTEIVN
                     INNHQYTVEYVPKEDIYPAYGYGGGDKAVVRQDLPPRVKKFVRSHELYHCQDKSDRGG
                     WLGKELRANLIPGLKDPVGLAVTIWATVTDIDRIKFYLKRTREGC"
     gene            18202..19443
                     /gene="glyA"
                     /locus_tag="PKK65_01820"
     CDS             18202..19443
                     /gene="glyA"
                     /locus_tag="PKK65_01820"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNU81452.1"
                     /translation="MKNDILFKKDSVVADIINREEKRQNEELELIASENYASPAVLSA
                     LSSVFSNKYSEGYPGKRYYGGNLIVDEIENLAISRAKELFCAEHVNVQPLSGSPANAA
                     VYMAFLEPGDKVLGLRLDHGGHLSHGHSVNFSGRLYNFIQYGVDATTELIDMAEVRRI
                     ALAERPKMIVAGFSAYSREIDWAGFKKIADEIGAYTFADIAHIAGLIAGKQLNNPVPL
                     FDVVSATTHKTLRGPRGAIIMCKEKYAKQVDRAVFPGIQGGPHDNVTAAKAVAFGEAL
                     LPEFQEYSRQVIINAQTLARLLVSRGYKIVSGGTDNHLLVLDLSPLNITGKEAETALE
                     AVGISVSRSTIPNDPNPPLNPSGLRLGTPAVTTRGMQTKEMEIIADIIDKAIKSRQQT
                     DKLIELKRVVSDLCQRFPIPA"
     gene            19446..20294
                     /locus_tag="PKK65_01825"
     CDS             19446..20294
                     /locus_tag="PKK65_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HNU81453.1"
                     /translation="MKKTAEIIDGKIIAKTIKDDIALEIFNHHSHRPNLAIILVGERP
                     DSELYVSLKEREAKKVGIDTHLYRFDNNVKESELLETISFLNNDTMINAILVQLPLPE
                     TMDANKIISSINPLKDADGFHLLHPDYVVSPVIAAVDFVVKKYQAKGRACVFYRSEVF
                     GQGLNKHLQALGLEVDLLAVNDDEDPRQNKELKKRLSAVSVKSGVVVTALGLSHFLDQ
                     DYCREGAILIDVGISKENGVVLGDVDFKSVAQKASAITPVPGGVGPMTIAFLFRNVWE
                     IYKHNN"
     gene            20335..22125
                     /locus_tag="PKK65_01830"
     CDS             20335..22125
                     /locus_tag="PKK65_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HNU81454.1"
                     /translation="MPTWLYLSFYQMIAKKAQLKELLKIHFGYDSFRPGQEKAIDAIL
                     EGRHTITVLPTGGGKSLIFQLPALVLEGVTLVVSPLIALMKDQVDSMEKVGIPATFIN
                     SSVSLSETVQRMKKIRQGYYRLVYIAPERFYNQSFLAELKNVKVSLFAIDEAHCISQW
                     GHDFRPSYLRLKEAIKYCGSPVVAALTATATPEVRADIAKQLGLTDYENIISGFARPN
                     LHFAAVVVNNGRKLEYILDVVRSQGGGSGIIYVGTRSKADEIVEFLSDYDVTAVAYHA
                     GMDSNSRDWVQEQFMQGKAQVVVATNAFGLGINKKDIRFVIHHDLPGTIEAYYQEAGR
                     AGRDGLDSYCLLFYSPADRYLQEFFISGDNPSPNLIKEIYRFLLEAQENNPDDENSVL
                     ITYAELAKHLSEPAPEMAIGTAIKILEKERYVSRPNERTGNTFIKLINSWEETLAAIS
                     SRAKVQLAVAEKLRHHFAREIEEGWQFVAEEAAIVLQIKKEMLNRAIKKMAEQGLLIY
                     RPPFKGTEIRLLKFSDPDDLVLDFKALEIKAERAKYKLDEMEAYVYSSACRQQYILKY
                     FGEKDAPVCGHCDNCLKQGKEVSRQYLGDY"
     gene            22127..22822
                     /locus_tag="PKK65_01835"
     CDS             22127..22822
                     /locus_tag="PKK65_01835"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNU81455.1"
                     /translation="MRKRRLEKLKKIVADSYEMTAEHFNNTRSKVAASDFQWAAKQIG
                     INDRVFDAGCGNGRLLDYVQIAPINYLGFDQSNKLLSFARRQHPGYKFESGDLSMLKN
                     LEPASFSVIFCSAVLSHFPSKRERIDLLRSFYNLSSVKGRLIISFWRMRGSYLRLIFI
                     NRLKKIIGLYPYGWRDLVFFWKDNQGRPISLRYYYIYSPRKFKKDILAAGWKIERVYK
                     DSFNYWVVAVKNS"
     gene            22875..23120
                     /locus_tag="PKK65_01840"
     CDS             22875..23120
                     /locus_tag="PKK65_01840"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HNU81456.1"
                     /translation="MKTTEQRINNIIGQLEGIKRMLASTPEDCFALLTQMKAVKSAMC
                     SLTEQILSSEFDRCLSGRMAADKRKKMEVIFKEVIKK"
     gene            23177..23506
                     /gene="trxA"
                     /locus_tag="PKK65_01845"
     CDS             23177..23506
                     /gene="trxA"
                     /locus_tag="PKK65_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNU81457.1"
                     /translation="MSKVVDIVKNNFEAEVLKSDVPVLVDFWAPWCMPCRMMAPILDE
                     LAGELEGKIKIAKVDTENPENQELAIQYQIQSIPNMKLFKGGQVVAEFIGMRSQDALK
                     QDLEKYL"
     gene            23565..24797
                     /locus_tag="PKK65_01850"
     CDS             23565..24797
                     /locus_tag="PKK65_01850"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNU81458.1"
                     /translation="MSIFQDNLERLQALASLDGISSEDLALLSKPALVSKTELEVGGN
                     KYPAWRILYSHDLGPGKGGIRFHPEVSEDEVQSLAFWMTLKNSLADIPYGGAKGGVKF
                     NPKQVDKKELEAISRAYARAFQPVLGQDKDVPAPDVYTNSQTMAWILDEYESIVGHHE
                     PGMITGKPVELGGLKLRSDSTAQGGYFIAQEVVKEFLTGKEAKDIRVAIQGFGNAGLF
                     MAEKLFVDGFKIVAISDSRGGILKEDGLDVAALSVFKAGGKSVSDFDQGQVISNEELL
                     ESDVDILILAALENQITAVNADKIKAKHIIELANGPIDLAADKILDEAGKVIVPDILA
                     NSGGVIVSYFEWAQNRTGQILDEDYLKNLLEKKMRTNWGRILSLYKEKNNISLRQAAY
                     ILAVRRILAAKHWRGGRV"
     gene            complement(24863..25870)
                     /locus_tag="PKK65_01855"
     CDS             complement(24863..25870)
                     /locus_tag="PKK65_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81459.1"
                     /translation="MKKICNRLFKNKKALAIVIAVLILVIAAVIATVIADKKPYKEID
                     IAEAVAKSEDFINQFLMPSGSKASVIETSEEYDLYKLKINIGSESPVESYVSKDGKLF
                     FPQAIDMDKIAQEQAGNNNSASNPTVPVDIPKNDKPVVELFVMSYCPYGTQIEKGILP
                     VLETLGNKIDFQLKFVDYAMHEKKELDENLVQYCIQKEQPEKLNAYLQCFLADGNGSD
                     CLSKNTDKNKIDSCVAATDKEYKVTANYNSKTDWQGNFPGFAVNKADNEKYNVGGSPT
                     LIINGTNVQSGRDSASLLASICSAFNNAPEECGQQLNTQSPTPGFGFDYSNTNTDVSC
                     N"
     gene            complement(25946..26719)
                     /locus_tag="PKK65_01860"
     CDS             complement(25946..26719)
                     /locus_tag="PKK65_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HNU81460.1"
                     /translation="MKTIHSHAGDIQIIYESGDFLVINKPAGLIVHKATGNKSKALID
                     YILESYPEIKKVGEDKSRPGIVHRLDKDASGLMIIARNNQAFVAIKQQFKKRTIYKEY
                     LALVHGQIIKDEGEIIFPLTRSKSGHKMAALPASNHAKKKLSNRDEGNIKARLKAKKA
                     LSLFTVEKKWPHFSLVSVNIKTGRTHQIRAHFAAYGHALVGDDLYGTNKNKLKNKKMS
                     LGRIFLVAKKLKFKDREGNTQEFQIDLPSDLKKIIDTLK"
     gene            complement(26721..28205)
                     /locus_tag="PKK65_01865"
     CDS             complement(26721..28205)
                     /locus_tag="PKK65_01865"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HNU81461.1"
                     /translation="MLYYNHYIKSFLKKQENMSLQQKIFSNTLVQIIGKLASTGLNLF
                     AFALMTRELGQNGFGEYTTIITFLSFFAIMADLGLTLVTVQMISGDQEKENLILGNLF
                     GLRLISSLIFIGLAPIIVQLANYNNSIKLGVLITAASFVFNALNQIMVGLFQTRLKMN
                     RVAWAETLSRFVMIAGVIISINLNWGLNGMLIATVSASLTSFLLHFIFAHRFAKIKPL
                     FNWPVWQNILNLSWPLAITIAFNLIYLRADTLILSLVKTADTVGLYGAAYKIIDVLSS
                     LPFMFAGIILPIMITTWRQNKEKFYKILQKSFDIMAAAAIPLVVGTQFLADKIIVLIA
                     GPDFLEAGIILRILIFAIAAIFMGNMMAHAIIAIKAQKKVIWIYVFTSITSLVAYIIL
                     IPRFSYFGAAAVTIYSEATIAVLSTWFIYRHLGFRICWRGTLKALIASLLMGALLWIW
                     PSTWTNSALGLLLTIAAAGLFYILFLGLLKAFRRSDLLLIFNKK"
     gene            28213..28647
                     /locus_tag="PKK65_01870"
     CDS             28213..28647
                     /locus_tag="PKK65_01870"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81462.1"
                     /translation="MSTTIKKTLSAFFLLFFGLLQIGLFGPPQAAIADSSLINSQEGF
                     KDKEIQAAFGNDTPDDIRITMVRIIMIVLSTLGVVFLALLLLAGFKYMTAAGDEEKVK
                     EAIKQITQAVIGLVIILSAWGISYFILLRIRAAITGTNYLYF"
BASE COUNT         8631 a         4814 c         5636 g         9477 t
ORIGIN      
        1 tatttattaa acaattataa ccaacataac actaattttt taaaaggtca atataatata
       61 gataatataa aactaattta tttattcact atttataaac accttataaa cataaattgt
      121 ccttgtggat atgtgtttta tctcctataa tatagttata ctatccgctt tatgaataac
      181 gatcaaattt ggcaagcagt cttgggtgag ttagaaataa atctttcccg agttaacttt
      241 attacctggt ttaaagacac ttttctgtct tcttttgaaa acgggcgagc cattatttgt
      301 gtacccaatg cttttgtaaa aaaatggcta gaagaaaaat accaccgcaa tattgtaagc
      361 tcccttgaaa atatcaccaa acaaaaatta aacgaaatta tctataaaat agagctacgc
      421 cacggcaaac aagaaaacgt tatcacgctt gaaaacgtgg ctataccaaa atcacaagac
      481 aagccaaagg aaaatattgt gagccaaaac gtagatagat ttggactaaa tcccaaatat
      541 gtattcgata attttgtggt tgggcggggc aatgaattag cccacgccgc ttgccgggcg
      601 gtagtaaata atttaggaaa agcatacaac cctttattta tatacggcgg tgtaggccta
      661 ggaaaaactc atttattgca agctgtcggc aatgaggcgg caaaaaaaac cgacaaaata
      721 ctttatacta ccagtgaaaa attcaccaat aactatatcc aagcggttca aacgggaaaa
      781 gctaaagatt ttaaaaacat gtaccgcaac gtcgacgttc ttctagtgga tgatgtccag
      841 tttatggggg gaaaagacgg cacccaagaa gaattttttc acacttttaa tgaactacaa
      901 caaagtgata aacaaattgt tatgacgtcg gataggcctc caaaatctat ccctgccatt
      961 gaaaaacgtc taatctcccg cttcgaatca ggaatggtcg ctgatgtggg caagcccgac
     1021 attgaaacta aaatagcaat cttagaacaa aaatctttag aaaaaaactt tcctttagat
     1081 aaagatgttt taatgtatat agctaacaat gttcaaaata atattcgtga attagaggga
     1141 gctcttaata aaattatagt tattcaccaa cttaacaact ccataccaac tctaaaaagt
     1201 gtaaaaaacg ctcttagcga ctatgtttca agcttacaag taaagtcctt aaccccaaaa
     1261 gaaataatag aagttgtttc ccgtttttat gaaattagtg ttaaagatat tattggcaat
     1321 agccgccgca aagaattagt tggccccaga caagtagcaa tctttctgat tagagaagag
     1381 cttaacactt catatccaag tattggtcaa gaaatgggtg gtcgcgacca caccaccgct
     1441 atgcacgcct ataataaaat tcttaatgag ataaaagaaa atgataatga aaaactaaga
     1501 caagaaattg aatctattaa acaattattc aataattcag cctattaaag tggaatgttt
     1561 gtggaaaaac tcataaaaac tgttaacttc ctgggattta agtgctgaat aaaaggtgga
     1621 aaaattaaag ttatcttcaa taaaaaatta atccccaaaa ttatcaccct aaaatcacca
     1681 catattaaga gttaaaaaca caaagaaaat aatttaataa tagataaaat aaaccacaaa
     1741 aatcatttat ccacaatact tattattact attattaaat atatataaaa tataatatat
     1801 aacagatatg aaatttatct gcctgcaaga aaatttaaaa aaggggttag cgattgttaa
     1861 tcatattaac aataaaaata ttaatttacc catattaaat aatattttaa ttagggctaa
     1921 tgataatgat aatagtattg agctaattag cactaatttg gaaattggaa ttactcacca
     1981 attaagagga aaaatagagg aaggcggaga aaccacgatc gacgcaaaaa ttattagtga
     2041 ttatgtcaat cttttaccgg aagaaaaaat agaatttaag gaggacaata atgaaattaa
     2101 tataaaatgt gaaaattata aaactaaggt taagggcgaa tcatctaaag aatacccatt
     2161 aatacccgtg attaatcgta ataatttttg tgagattaat atttttgatt ttcgcaaagc
     2221 tctaaatagt gtagtttttg cagtagctaa taatgataat cgtatagaat tgtctggtgt
     2281 atttttccaa ttccaaagcg atgttttaac gattgcggct acggatagtt atcgtctagc
     2341 tgaaaaaaaa ataaaaataa aaaataactc cattaatgaa actaacatta ttgtccctgc
     2401 tcgcaccatt caagagattt taagaattat ttctaatttt agttctgacg ctcagtcttt
     2461 agagtctgat attattaaaa tttatttgtc tgataatcaa attcttttct ctttagattc
     2521 ggtggaattg atttctcgat taataaatgg tcagtatcct gattaccagc aaattattcc
     2581 tgataaatat aagactgaag tggagataaa gagacaagat ttaataagag ccattaaagc
     2641 ttccgctatt ttttctaaat ctggtattta tgatatttct ttaattttta agaaaggggt
     2701 tgttaatgtt tatgcttcat caggtcaaag tggtgaaagc tctattgatt taaacggcga
     2761 tgtctctggt gatgataatg acattactat taattgtcgt tatttattag atggcttaaa
     2821 tataatagat agcgaacagg taataataaa attaattaat aataataccc cttgtgccgt
     2881 tgtttccagg gatgatgatg actatcttta tatagttatg ccgattagaa attaataata
     2941 atttttataa aataaggaga ctccacataa aaaagccgtt cttgaaagag ggcggctttt
     3001 tggtggggag aagagattgt tgttttattt ttaataaact aaattaaaat ttatttcggc
     3061 attattacaa ttacccacat cgtcgcaggc ggtggctgtt attttttgat atcctttttc
     3121 taatccatct aagaaaaggt ctatttctgt ttctgcgccc agtccgggga gagcggaacc
     3181 atttaactga tatttaattg attttatacc ccttggagcc gaggcattaa ctttaacctt
     3241 taatattttt ttgtttacca attcaccttc ggacggcgat tcgattatta tagttggctt
     3301 attttctggt tttataagtt cagcctggtt aatcaagtct tcgcttgata gttggttatt
     3361 atcttttgcc catcttttta ctgcttcttc ccaggaatta aattgagggt cttctgttgg
     3421 attttctggt tccggaccca acgggtcttc tttgttgaca tagtagagga tggtgtggtg
     3481 attaaatatt tcaatttctt cttttaattc atctggggtg tcgctatttg ccggtagttt
     3541 agtttctttg ttgatgataa tttttttaga aaattgtata gtgccgtcta tcgccgcctt
     3601 tccagtggcc tcttcttgcc aaggattaaa tgattctact ggtgtaccgg tgagggcgcg
     3661 cctcataaat tcattccaaa taggggcagc aaccacactg ccatcagcgc cgctattcat
     3721 tttggaatta tcagtatttc ccacccagac gccggcaact aaagatggcg tgtatccgat
     3781 tgtccaggcg tctttataat cattagtggt tcctgttttg gcggcgacta cacggtcatt
     3841 gagggttagc caattttttg caccaaaaat gaaggctctg gcctcgttat ctgttaaaat
     3901 actgttaatc atttgggctg ttttagtggt gattacccgg ctttcttggt ttttatattc
     3961 ttccaacacc ttaccatcct tatcttctat ttttaaaata agcgatagtt tgtttatttt
     4021 tccctctctc gcaaagacgc tataggcatt gacgtgttct atcatcttca cttcgcctcc
     4081 gcccaaaact aaagataatc catatcgact gcggtctgtt aaggtggtgt aaccaaggtt
     4141 gtcggcaaga ttaagaactt tatctattcc tgctagatat atagccttaa ccgccggaat
     4201 gttaagagat ccagataaag cttgacgaat agagacaggt ccatattctt taccgttgta
     4261 attgtggggg gtgtatggtg cgccattatc tgtcgaaaaa ttagtgatga cgtcgtataa
     4321 ttttgtgtta ggagtataac ctctttcaaa tagggcagcg tatactattg gtttcattga
     4381 agatcccggt tgtctgggtc tatcggcaac gttgacttga ccgtcaattt cgtcattaaa
     4441 ataatctctt gaaccaacca ggctaagcac ttgtcctgtc tttgggtcta tggccactaa
     4501 ggcggcattg ttggcattat atttatcttc gtaattttca gtcttttctt ttattacttc
     4561 ctcggcaatt ttttgtttat tccaatcaat ggtggtgtat atttttaagc cacctctctc
     4621 cacctcattt tctccatatt tctctgataa aatatctttt accatcatca caaaatgagg
     4681 agcggtgatg ctggtttcag ctctttgaaa aactatttgg tagcttttgg cggcgtcttt
     4741 ttctgcgtta gatatatatc cttgttccgc cattaagtcg agaatatagt ctttgcgtcc
     4801 catgagaatg tcaacattgg gtccgtatgg ggaatagcgg cttggtgctt gcggtagggc
     4861 cgctaatagg gctgattctg ctaaattgat atcacgtacg ctttttccaa aaaatttttg
     4921 gctggcggcc tcaaccccat aggcgttaga gccgtagggg atttcgttaa gatacatttg
     4981 caaaatttca tctttactga attttttttc aattctttgg gctaatacta attctttaat
     5041 tttacgaata tagctttttt ccgaactgag tatggcattt ttaataaatt gttgagtaag
     5101 agtcgacccc cccgcctttc ggttgtagat tacattggta atagcggttc taaacattgc
     5161 ccagaggcta aaccctccgt gtttataaaa atttttatct tcaatagcaa ttgtggcgtg
     5221 ttttacattt tctggaatgt catcgagact aataagagtt cttttttctt ccccgtggat
     5281 ttcgtaaagg agatgctctc cacttcggtc ataaattttt gtgctttgag caatttctct
     5341 atctattagt tggttggggt taggcaaatc tcttgatacc caggccacta taataaaaac
     5401 cgacaataaa cccaaagaag cgagcaggat taaggctata ataattttat gttttatttt
     5461 tatctttttt ttctttggtg tttgtccttt acgccaaaat tctgctttag gcgcgtttac
     5521 tgtttttaat ttaggtatgg gcatattatt tccggtaata tgtagcggcc tcttccggca
     5581 tgacaggatt gataattagg ccggaaccaa gttctattcc cgcccagata ttggggcgag
     5641 gttgtatttt tatataaaaa tgttgatgtt ggttgtcgat atcgttgtga gcaaagaagt
     5701 taaaatcata ttccagagac tttaacttct tggctattag cttaagagct tgggccaatg
     5761 attttaattc tttattattt aagaggctaa tattatctac atgtctttta ctgataatcc
     5821 agacttcata tggaaattca ctggcgaagg ggcaaatagc tatcatttta ctgtcttgcc
     5881 agactaatct ttggcttttt tgttcgttat ttattaatag gcagtagggg caaactttgt
     5941 tttctgcttg gtatcgggcg gttctgtttc tttcctcgat tatttccggt ggtagaattg
     6001 aagatgcaaa tatttgggaa tgaatatggc gcaatgaggc gccggcggca cgaccttggt
     6061 ttttaaaggt cagaatgtac ttaatttttt tgtctttaga tagcgcttga ttgcgtagtt
     6121 gaaataattt aagtaaagaa cttatttgtt ttaaattaag ttctaaaaaa ttttcataat
     6181 gatgtccaga atcaataatt atttcttgat agccgtaggc gtttttgttt ttatttgata
     6241 gtgcggggta aatatttttt atcgccgcgc tttgccattt tcctgaagat ggccattgtt
     6301 gtaaaatatt ggtttttttt acattagcgg agcaaaaggg acaagaggat tctttagttt
     6361 tattttttag gtcattcggc ctttttgctc ttttgggtgc aaaaatgata ctccggccgt
     6421 ttaaggggtt gtatctaatt tgagcttgat ttttcatatt attttttatt taacttataa
     6481 acattatatc agatttaact ttaaataaaa aaacgggctt agttttgccc gccccttaag
     6541 cttgctggca caaaattgtc cgttcttggt ttttgccgtt tttgatcaca aatttagcta
     6601 ttttaaggct ttatttcaca ttagccagac gattttttcg tgaccttttg gctattggtt
     6661 gttactcttt gggtgtgtcc gctatttctg cggcttggta catacaacaa gagagtaaat
     6721 tgttaatccc tgtaacctta gattgctggg ttgagatggt tttttcttcg gtgtttatct
     6781 ttttaatttt tgagggttgt tttaagtaat aaaaaatcgg tccaaaatga gggccgattt
     6841 ctattttctt aacacgacaa ttattttatc agatgttaaa attttgtcaa ataagatata
     6901 atgtctaggt atggacacaa caattaccag cctacaaaat cccttgatta agaatattgt
     6961 aaaattaaga aaagtcaggt caagacagaa acagaaaata gtcgtcgtag acggccttag
     7021 agagattaag gtggctttta attatggctt tgaatttata gaaattttat attgtccaga
     7081 gttaagccaa gaaacgtgga cacaggcgcc tttaaggccc ataatagtgt ctaaagaggt
     7141 tttttttaag attgcctacg ccgataatcc caacggttgg ctggccttgg tgcgcccacg
     7201 ctaccgttct ttatctgatt taaagattaa gactaaccct ttaattttag tgctagaggc
     7261 ggttgagaag ccaggaaatt taggcgcaat gttacgaact gcctatgctg ctaaggttga
     7321 ggcggttatt ttaaataatt tacagacaga tttatataat cccaatgtga ttaaggctag
     7381 ttctggttat gtttttagtg atcaagtagt tttggcgagc caggaagata cgctggattg
     7441 gttaaaatct aaaaatatta agatttttgc caccaatatt aaggcggcaa aaaaatatac
     7501 tgatgttgac tggaggcaag cggcagcttt gattatgggc actgaggcca ccggtttaag
     7561 tgatttttgg attaataatg cggataataa tataattatt cctatgcaag ccggcatcga
     7621 ttctttaaat gtttctgtta gcgccggtat tttaatcttt gaagctagac gtcagcgtga
     7681 cttgggtaaa taatgctaat tttcgttagt attatctatt tgacagtctt gtttttatct
     7741 tgtattatct ggttattatc aagagtctgc gcccatagtt caatggatag aacactagcc
     7801 ttctaagctg gatatgtagg ttcgattcct actgggcgca caaagttaat ttttaaatta
     7861 aatttgttta ttattatatg gaaaataaga aaaaagcagc actggttaaa aaaataacta
     7921 aggccaaaac aataaaaaaa gaaccggttg ccaaaaagaa aaccagagaa gaaattgaaa
     7981 aaatgtttgc ttttaatttt ggatgtgatt gcagttgttg ttctcatcgt tgcgattctt
     8041 ataaatagtt taaccttgtt tctatgtatg gtgcctatgg tgtagtggta acacagcagg
     8101 ttgtggtcct gttgtttcgg gttcgattcc cgatgggcac cctaaacggt atctaatata
     8161 accgagtccg gttgattgga tatcatgatg gcagaaatct cggcaatttg tcgggatttt
     8221 tgcgttttgg tgctaattaa tggctttgat ttgccagcgg cggtctggat tttcttgttg
     8281 gctatctcga atttctctca gcacactttt tcagatttaa attggaagtt acttttaagg
     8341 ttatttccag tttgcttttc cggcataaga tacttttgtt ttattttgac taaaaatagt
     8401 aaatatcagt aaaaatgctt tattttagtc aaatatacta tctattcagc tattatatca
     8461 acatgttgat atagtaattt gttgacttag catttcagat atgatacact aatgtcaaat
     8521 atatgaaaag caaaaagaat ttattagaac agttaaaaac ttttcctcac ttcaataaaa
     8581 ataccgtaca tcagcttggc agacagtggg agctcaagga ttctacgatt aacacttata
     8641 tcagtcggtt tttgaaatat aaggatgttt tgcctttaaa aaatggcctt tatgtgacta
     8701 ccgatttttt taaaaacaat cgatcggatg tttcctattc tttttatctg gcaaatatta
     8761 ttcgtactcc ttcatatgtg agctcttggg ccgccttaca gtattataat cttgctacag
     8821 aggccgttca ttctatcact tcaactaccc ttaaagtgac gagagaatat cagacgaaag
     8881 ccggcaattt tgcttatcag tctatcaaaa aagatttttt ttctgatttc tttttagtaa
     8941 agggtaaatt tgatttttat atcgcctctc cagctaaggc attgtttgat ttgctgtatt
     9001 tcaggacgcg tcagcttaaa ggaataaatt tagaagatat taaaaaaatg attgaggaat
     9061 taagaataga ttttgatgaa atggataaga ccgaacagga aaaattttat tcaataatta
     9121 aaaaacttat ataagcgaac aaataacgac aattttaaaa agaaaacttg acgatctgtc
     9181 tgcctacgga aatattgatg ctgaaactcg tcgcaatgct cttaaagagg agctgcagtt
     9241 ttatgttttg aattttatct gtcatcatcc agaatatagc aaatggataa tgtatggagg
     9301 ttccgccctt cgcataattc atggtctcga tcgtatgtcc gttgatttgg attttgaagt
     9361 tccccatgcc ataacagaaa aatttttaga agaattaaaa aaagaagtcg agggttattt
     9421 tgttaatact tacggcgccg attctgattt tcttaccata aaaatcacta ctggtagggg
     9481 gctactttta aaattccacg tcggtaaaga gttaagtctt ggcaatcctt caaatcaggt
     9541 gcatgtaaag attgatttaa accattttgt tgccccgaag accgtaactg aacgtcgacc
     9601 gataaatcgt gatcagcttt cgtttgttat tttgacatac aacatgagag cattaatggc
     9661 cagtaaatta gcggctattt tcttgcgagg ccctcgtggg gttggtgatg ttgtttatga
     9721 agaaaagggg cgtgacattt atgatcttct ttggtatatg ggtaaaaaaa tcgtaccaga
     9781 ctttgattat atgacagcca aaggcattga tgtaaaagat cctagaactt tatttgataa
     9841 gttaacatta caaatgaata aagttagcga taagaattta aaaaatgact tgacgccatt
     9901 atttgttgat caaaaattta tttctaattg gctcaaaaat tggagggaga gctattttag
     9961 attgcttaat gagtataaaa tacatactat aaaaaattta gaaagcgttg gcatatcacg
    10021 ggactttaat atggacgtat tttcggacgt attctctttt atatattggt ttaatacaga
    10081 agaggaaagt tcggttagag ttgtttatcg tatcagcgat tattggatta attttcgtga
    10141 cggcgacttg gctattgatg tagataaaaa attagaagat aaaatggagt ttaatggtaa
    10201 tggttggagt agccggccaa cttttcagga taagttaaag cggtatgcca ctctttttta
    10261 tcagaaaaca gaaaaatatt tcaaaaaaac gaatcacata atgcttggtg atgttattat
    10321 tactaaagta atcagaatga cggctgacaa tttaaaccag aaggagcaga tcgtgctcaa
    10381 taaatctgct ttgttatcat gtgaattgga tgatttatta aaataaacat atgtctcggg
    10441 agatcgccaa caaaaccaaa aagcaaattt ttatcaaatt taggatgtgt gaattaattt
    10501 tgggtaaaga tatgtaaaat attagccaag gtaatagtaa ataattattt taaaacccga
    10561 tagcaaggct atcgggtttt tatagttttt taatctcatt ttgtattttt tggcggacat
    10621 cattaatttc catcgaatcg aactttatgg ctggtccgat tttgattaaa attttttgtc
    10681 cgcctttgac gcggagccat ttaaagccaa tgtttattgg cattactttg tccatccccg
    10741 agattcttat tgggatggta accggttgta agtcaaccac taatttggcg ggaccatatt
    10801 tacaggcgcc aatttcgccg ttacgactgc gcgttccttc aaagaacaag agcaaattac
    10861 cattttccaa aatatttttg aattctttaa tatttcgatt ggctgtttgg atattgcttt
    10921 gtctgtaggc gggaattgtt tttaaaaagt agcagaatat tctgcgaatg ggttttttaa
    10981 aaaaattttc ccaagcggcc gcattccaag gaatttgccg ataatggcgg aaaatatccc
    11041 atgggctgaa aagggcgtaa ccgattaaca ttgagtcaat tagactctga tgattagaca
    11101 aaaagagtaa acttgttttt ttgggtatat tttctctgcc aataattgtt gttttatttt
    11161 ttattttaaa caaccaccag ccgagcaccc aaaaaatcag gagcatcatt acgctaatta
    11221 agtttttaat gagcattttc attttttctg gatttttggg gttaatattt atagtctaag
    11281 acttaataat atttttggca aattgtgtat tatttagttt taaacatgaa ccatttttat
    11341 cattttctca agtgattaac aatgaggaaa tggccaccga gattgtccgt tctttagtag
    11401 gcgctattgg cctttgttta actatgccga ttgctaccgg tctttctgtt tggctattat
    11461 ttaaagaaaa aaaatcatta aactaattat ctattttata aaatttacca ttctttgtaa
    11521 aaaagctata ataaagttaa aagagataag cttaagttat gcagtccgtt tttaagtgga
    11581 aaaaaatatt ttttagcctt ttagctctta attttgtctt aagcgctttt gttccttttc
    11641 taattaaatc tgactttcta ggattatcag aagaagctgt tgaggtgctt attatcgccc
    11701 ttcttttgat tttggcttat gtagttaatt ttttgtattt acgcgaagtt ggtcgattaa
    11761 agcggtatca ggtaaattta gaagatcgtt tgcaggatac ttttaaatat attggttctg
    11821 ttaatttaca aatagaagaa atgcgtcagg ctttcgctaa ttttaaaaaa tatccggaaa
    11881 gtaaaaaaga tatacgaact gtttttatat atttttcaga aaaaatatta agtattgtta
    11941 atgctgattg ggttatttta cgaattatcg acattaaaac cggacgaacc attcgcgatg
    12001 gtacctttac ccgcaataat cagaaaatta atcattttaa gtttgataat caagaaatac
    12061 tttccggcca gtgtaggtct gagcagtgta cgattgtaaa gtctgatcaa gaaaatctta
    12121 atattaaagc gtgttgtatt ttaccaagca agttaaatgg ccgcgatcaa gaattttttg
    12181 tccgttccat ggttaatcaa ctagaaatga tgtttattgt tttttcttct cttagtaacc
    12241 gtaaagtcgg caattctaaa aatcgcatta aaaagaaata atttgtctct aaaaaaagac
    12301 ctcatattga agcctttttt tgtttggtaa tattagcaag tttcaattaa ttgaagaatt
    12361 tcatccctct tcttttccat tacctctttt tcttttgcct ctaagttgag tctaacttta
    12421 tcttctgtat ttgagcctct gacattaaac cagaaatcag gataagtgac gctaactcca
    12481 tccaataagc taatattgcc gtcagcatat tttttttgaa tggcactaaa tatagcttct
    12541 ttgttgttta catggcgatt aatttctccg gagtgaaaat agcggcgata tggtcgaata
    12601 aaatcagagg ttgttaaatc gctttgtgaa aacaaggtga gcatttggcc aattattatc
    12661 attggcattt caaaacagcc aattgcgaga ttaagaaaaa aatggccaga agattcgcca
    12721 gcaaaataag cattttcagc cagcatcttt tcctttatta aagagtgtcc gacgcgagtg
    12781 ggaacaggaa tcccgcccgc ctcttcaatt aaatcaaccg taattttacc aggtctcaca
    12841 tcatagcaga ttttgctgcc aggtttatct tttaggaata tttgtgctaa caatcctctt
    12901 attatagcgg gatcaatcaa ttctccatga ttatcaacaa aaaagacccg gtcgccatcg
    12961 ccatcggtcg caatgcccaa gtcggcctct ttgttaataa ttgcttcttt taattcgttt
    13021 aaattttctt ctttaagcgg gtcagcctca tgtgccggga aagtgccgtc aagctgaaaa
    13081 tttagccttt ctagattaat tggtaatttt tcaaacagca aatctaaata ttgcgctccc
    13141 atgccgttag cggcgtcagc tattatccgt agaggcttaa tcgccggaat atcaataaat
    13201 tttaaatcat gttctaactg agatttggcc gcgtctgtta attgtctggc ggtagccttt
    13261 cctggcggca ttgctatttg gccaatattg tctttaagcc aattaatgcc gctgtcgcta
    13321 ctgacagggc gacttttggt tctaaccatt ttaaaaccat tccattcttt gggattatgg
    13381 gatgcggaaa tcataatgcc accgtccgca ttttgcttgg aaacattaaa ataaaaagcg
    13441 ggagtggaga ttagtccggc atctattaca tctgctcccg ccgaagttag tccagatatt
    13501 aggccttcat ttagggcggg cgagcttaac cgcatatctc tagccactaa gatttttatg
    13561 ggctgattta agggacaatc gccgtcaagc tgtcgtaaag agacaaaagc tcttcctagg
    13621 ttataggcaa aattaatatc aaaatcttta ccaaagacgc ctctaatatc ataggcttta
    13681 aagatttcgg gattaaacat atcttttttt tgtttttaaa tacataatta aagaacctaa
    13741 gcctaaacaa gtagcgctca aagaaattat ccagccgata aaaggtattg aaaataaaag
    13801 ccaagcgata gttacgccag cgagtagcgg tattaataaa gacttttctt tttttttgtt
    13861 taaaatctta tttactgctt tgccaataaa tatggcgcta ataattttgc ctaagtagag
    13921 aactaaaaac caaactaaaa atccgagaat ggctaagggt atgccgataa tggtaaaaat
    13981 taagattaag cttaaggttg gtgttgccaa taaaattaac atgccccagc ctagacttgc
    14041 ccagggattt ttttgggcgg tgtcgtgtag ggcgttgata atttttttgc cgggaaaaat
    14101 tataatcaag ccaataatga ttgccgctaa aattttataa ataatactaa aaaaataata
    14161 tttatttttt gttttttctt tattttttat taatttggtt tgtccggaga tttgagctcc
    14221 gtcttttttt aatattttta tattttctga ataattcaaa tctccaccaa tatttgcttc
    14281 ggagctcaaa gaaattccag aatttttgtt gccgataaat tctaagtttt tacccacaac
    14341 cccggagaga acaatattat tcccatttcc atagacattg ccgttaatta agccggcaat
    14401 gtttatatta ttagccccgc tcattaggtc ccagcctatt tttgcatttt tatttatatc
    14461 taaagcgttg ccgataaaat taagatttct agcaaccgtt ccgtttattt gggcgctatt
    14521 gccagctaag cggacgttac cgtttacttc tccgtttact attatggtct gggcggcggc
    14581 aatcaaatcg ccgtctaagc gtccgtttac tattatagtt tgggcggcgg caatcaaatc
    14641 gcctttgatt tctccatcaa tcgtgatatt attgctggcg gcgtacaaat tgccttctat
    14701 ggtctcctgg ggagggaggt agacgttatc cccagactta atgtcagccg ctgaaactaa
    14761 cagagggcta agtattaata gcccgccgat tatcaaggat agtttaatat ttttcaacat
    14821 aggataataa ttaataaaat ttgttataat aacagtatta tatcgtaaaa acatgataga
    14881 agcaattaga gaattaattg aaagagcgca aatttccgtt aaaaatcggg ataatttatt
    14941 atctttaaaa agagaaatta gcaaaaaata taagatagat tctcttttaa atgccgatat
    15001 tattaaggaa tacaagcgtc tactttcttt aaaattaata gattctaatt taatattaga
    15061 gaatttatta cgcaaacgca ctattcgtac tttgtcggga attgctcctg tggcagtttt
    15121 aaccaagcct tatccttgcc cggggacctg tgcctattgt ccgcgtcaaa aaaaagtacc
    15181 agtaagttat ttgtctaatg aaccggcggt aatgcgcgct attcgttgcg gttatcatcc
    15241 ttatacccaa acagcttctc gtttgcgagc tttagaaaat aatggtcatc aaccagagaa
    15301 aattgagtta atagttatcg ggggcacttg gagttatttg ccagtaaaat ataaatattg
    15361 gtatgtgctc aattgtttta gagcggctaa cgattatctt tcttttggag aactaaaaaa
    15421 gtctccttat cgggcgactt tacatacagc tgaattaaaa cgattattgc tctgggaaca
    15481 aaaaaagaac gaaaaggctc gttatcgaat tattgggctt actctagaaa ctcggcccga
    15541 ttatattaat accaaagaat tggaagaaat gcgccttctt ggttgtacgc gcgtagagat
    15601 gggcgtacag attacggatg ataaaatttt aactctaaat agacgcggac atggcgttaa
    15661 ggagattgcg gaggccaccg ctcttttgaa aaagtacggt tttaaaataa cctatcatat
    15721 tatgccagcc ctacccggct ctaatccaaa acttgatttt caactttttt cgggcttatt
    15781 taaagacgaa agatttcaac ccgatcagat aaaattttat cctacggtag tgacggcagg
    15841 tagtttgtta tataagtggt atcaacaagg caagtataag ccttattcgg acaaagttct
    15901 ccaaaaatta attattaaat gtaaggcggt tgtgccggag tatgtccgta ttatccgttt
    15961 aattcgtgat attccctctg aatctattat cgctggcaat aaaattacta atttgcgcca
    16021 aattatgaaa cagcagggtg tttattgtcg ctgtatccgc tgtcgagaag ccaaagacgc
    16081 gcccattagt tcatattcta tcaatattca aaaatatcaa gccgccggag gctgggaata
    16141 ttttattagc gccgacagtt tagacaaatc tattcttttc ggcttttgcc gtcttcgttt
    16201 ggataaatct agtccaattg ctcctgctat tgtcagggag ctccatgttt atggtgaatt
    16261 aacacccttc ggtaccggtt ttaaagtcca gcatcgcggc ttaggtaagg aattattggt
    16321 aaaagcggag caattagctc tgacggctaa ctctgataaa attgcggtca tctctggtgt
    16381 tggggtgagg gattattatc gccgtcgcgg gtatcgttta tataatacat atatgatgaa
    16441 aaaattaaat tagtaatttt accaacgaat ataaaattat atagcatcat tgcaaaacac
    16501 taatcaattg attgaaaaaa atcgcaatgg gcatcggccg gcacctcagc ttgagcctca
    16561 gtgcaaaaac cctttccatc ttccataatt tgaaaactat tgcaacgttg gcattgagaa
    16621 ttttcattct cccagccata aacaaagttc gcttcaattt cgtgagacat aaataaaaaa
    16681 taattaaaat attaaaacta actatttatt aaaaaccaag ccttccctct cactcactac
    16741 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctaattttt taaaatcagg
    16861 atgaacttga gcggtcacac taaccgcatc accactggta gataaattgg cattatctaa
    16921 taaatataaa cactgaccgg atggtaaaat ccatctttaa atgttttagt tttttttgaa
    16981 ttcgaggatc attcaccttt aattgcgtat cacgtccaac agtaataaat tgcgtttgtt
    17041 ttaattttaa taattggaga acatttgaat taccaccacc gacaatactt aaaggtaggc
    17101 gttcatagac agaaattatc tttttatcaa gccgaataat aaacttccta taaaaaataa
    17161 cccctattcg ccagcgggta taagggctaa ttttatttgg tgggcaaagc tgaacatttt
    17221 ggaatagttt tatataaaaa cattaacggt taacaaccct ctctcgtccg ttttaaataa
    17281 aatttgattc tatcaatatc tgtgactgtc gcccaaatag tcaccgctaa tcctactgga
    17341 tctttcagac caggaattaa attagcacgt agttcttttc ccaaccaacc accacgatca
    17401 gatttatcct ggcaatggta taattcgtga gatctaacga atttttttac tcgaggcggt
    17461 aaatcctgtc taaccactgc cttatcccca ccgccataac catatgccgg ataaatatct
    17521 tctttgggta catactcaac agtatattgg tggttattaa tatttactat ttccgtaaga
    17581 ttaggggatg ggttttttat attattattc ctcgacggtt ctattttttc tttatctatt
    17641 tgaagatgat tttttccaat atccttatta tcctcaggtt ttaaattttc aatttgaaga
    17701 cgaatctttt ccagctcttt attgtcttcg acttttaact cttcaatttt tgagctagaa
    17761 agctcttggt tagcttcatc ggactgaaac aattctgata cagaataaaa agaatcttct
    17821 ttagctgtta tattcttttc cagttttggc attgacattt tttccatata aaatgagata
    17881 ctagtcaaaa atatttcaat aatattgctt catttagtaa atattcgtaa ttaatattat
    17941 aacatacaga cctaaaacga taaactgtcc gttatggata caaataacgg ttaccgaaac
    18001 ctatcgagta gatactaatt cggttactat ggtgatcata tgaatatgtc tcttatttta
    18061 gagccgtgag cttaaacttc gttgtattct ataatcaatg attttataat tgttttttga
    18121 ttttagtttg attttattta aagcgccctt ggggtatttt tatttttgac aaaagagcct
    18181 ctctaaacta agattataaa tatgaaaaac gatattttat ttaaaaaaga ttcggttgtt
    18241 gccgatatta tcaatcggga agaaaaaagg cagaatgaag agttggaact gattgcttcg
    18301 gaaaattatg cttccccggc cgtcctttcc gccctctctt cggtgtttag taataaatat
    18361 agtgaaggtt atcctggcaa gcgttattac ggggggaatt taattgttga tgagattgaa
    18421 aatttagcta tttctagggc taaagagtta ttctgtgctg aacatgtaaa tgttcagcct
    18481 ctttccggat ctccagctaa cgcggcggtt tatatggcat ttttagagcc gggagataag
    18541 gttttggggc ttcgtttaga tcatggcggg cacctgtctc acggtcatag cgttaatttt
    18601 tctggacgtt tatataattt tatccagtat ggcgtcgatg cgaccaccga actaattgat
    18661 atggctgagg ttagacgtat cgctttggcg gaaagaccga aaatgatagt cgctggtttt
    18721 agcgcttatt cccgagaaat tgactgggct ggttttaaga aaattgcgga tgagattggg
    18781 gcgtatactt ttgccgatat tgctcatatt gccggcctta ttgcgggcaa gcaattaaat
    18841 aaccccgtgc ctttatttga tgtggtcagc gccactactc ataaaacttt acgcggaccg
    18901 aggggggcaa ttataatgtg taaagaaaag tatgcaaaac aggtagatag agcagttttt
    18961 cccggtatac aaggcggacc gcatgataat gttaccgccg ccaaagccgt tgctttcggc
    19021 gaggcgctgc tgccagaatt tcaagagtat tctcgacagg tgattattaa tgctcaaact
    19081 ctggcacggt tattagttag tcgaggttat aaaattgttt ccggcggcac agataatcat
    19141 cttttggttt tggatttatc tcctttaaat attactggta aagaggctga gacagcttta
    19201 gaagctgtcg gcatttctgt ttctcgatcc actatcccta atgatcctaa tccgccatta
    19261 aatccttccg gtttgcgctt aggaacgcca gcggtgacga cgcgcggcat gcagactaaa
    19321 gaaatggaaa taattgccga tattattgat aaagcaataa agagtcggca acaaactgat
    19381 aaacttattg aattaaaacg cgttgtttct gatttgtgtc agcgttttcc aattcctgct
    19441 taaatatgaa aaaaacggct gaaattatag atggaaaaat aattgctaaa accataaagg
    19501 acgacattgc tttggaaata tttaatcacc actcccatcg ccctaatttg gctattattt
    19561 tagtgggtga gcgtccagat tcagaattat atgtatcttt gaaggaacgt gaagctaaaa
    19621 aggttggcat tgatacgcat ctttatcgtt ttgataataa tgtaaaagag agtgagttgt
    19681 tagaaactat tagcttttta aataatgata caatgattaa tgcgatttta gtgcaactac
    19741 ccttaccaga aacaatggat gccaataaaa ttatcagctc tattaatcct ttaaaagacg
    19801 cggatggttt tcatctttta catcccgatt atgttgtttc tccggttatt gccgcggttg
    19861 attttgttgt taaaaaatat caagcgaagg ggcgggcttg tgttttttac cgctcagaag
    19921 tgtttggcca aggcttaaac aaacatttac aggcattggg gttggaggtt gacttattgg
    19981 cggttaacga cgatgaagat cctcgccaaa ataaagagtt gaaaaagcgc ctgagtgcgg
    20041 ttagtgttaa aagtggtgtt gttgttaccg ctctcggtct ttctcatttt ttagatcagg
    20101 attattgtcg agaaggagcg atactaattg atgttggtat ttctaaagaa aatggcgtcg
    20161 ttttgggcga tgttgatttt aagagcgtag cccaaaaagc ttcggcaatt acgcctgttc
    20221 ctggcggagt agggccaatg acaattgcgt ttttatttcg aaacgtctgg gaaatatata
    20281 agcataataa ttaatttaga ctggagattg ttgcggttgc tattaaattt tgccatgcca
    20341 acatggcttt atttatcttt ttatcaaatg attgctaaaa aagcacaatt aaaagaatta
    20401 ttaaaaattc attttggtta tgattctttt cgtcctgggc aggaaaaagc gattgatgct
    20461 attttagaag gtagacatac aattactgtt ttgccgaccg gaggtggcaa gtctttaatt
    20521 tttcaattgc cagcacttgt tttagaggga gtcaccttgg ttgtttcccc tctgattgcc
    20581 ttaatgaaag accaagttga ttcaatggag aaagtcggaa ttccggctac ttttattaat
    20641 tcctccgttt ctttaagtga aaccgttcag agaatgaaaa agattagaca gggttattat
    20701 cgtttagtct atattgcgcc ggaaagattt tataatcaat catttttggc ggaattaaag
    20761 aatgttaaag tttctctgtt tgcgattgat gaggcgcact gtataagtca atggggtcat
    20821 gattttcgtc ccagttattt acgcttgaag gaggcgatta aatattgtgg ttcgcccgtg
    20881 gttgccgctt taacagcgac ggccactccg gaggttcgag ctgatattgc caaacaactc
    20941 ggtctgacgg attatgagaa tattattagt ggttttgctc ggcctaattt acactttgct
    21001 gcagttgtag ttaataacgg acgcaagctt gaatatattc ttgacgtcgt ccgcagccaa
    21061 gggggcggtt cgggtataat ttatgtcggc acacgctcaa aagccgatga aattgttgaa
    21121 tttttgtcag attatgacgt aaccgctgtc gcttatcatg ccggcatgga tagcaatagc
    21181 agggattggg tgcaagaaca gtttatgcaa ggaaaggcgc aagtggtcgt cgccactaac
    21241 gccttcggct tagggattaa caagaaagat attcgtttcg ttattcacca tgatttgcct
    21301 ggcactattg aagcctatta tcaagaggct ggccgggctg gtcgcgacgg tcttgatagt
    21361 tattgcctac tattttattc ccccgctgat cgttatttgc aagagttttt tatttccggc
    21421 gataatccca gccctaattt aattaaagaa atttatcgtt ttttattgga ggctcaggaa
    21481 aataatcctg atgacgaaaa ttcagttctc attacttacg ccgaactcgc taagcatcta
    21541 tccgagcctg ctccggaaat ggctattggt acggcgatta aaattttaga aaaagaaaga
    21601 tatgtttctc gtccgaatga gcgcactggc aacactttca ttaaattaat taattcatgg
    21661 gaagaaacat tagcagctat tagctctcgt gccaaagtac agttagcggt agcggaaaaa
    21721 ttacgccatc attttgctcg agaaattgaa gagggctggc aattcgtggc cgaagaagcg
    21781 gcgattgtct tgcagataaa aaaagaaatg ttaaatcgcg ccattaaaaa aatggcggaa
    21841 caaggtttgc ttatatatcg tccgcctttt aagggaacag aaattaggct tttaaaattt
    21901 agcgatcctg acgatttagt tttagatttt aaagccctag aaatcaaagc ggaaagagct
    21961 aaatataaat tagatgaaat ggaagcttat gtttattctt ctgcctgtcg tcagcagtat
    22021 attttgaaat attttggcga gaaagatgcg cctgtctgtg gtcattgcga taattgttta
    22081 aaacaaggca aggaggtaag tagacaatat ttaggagatt attaatatga ggaagaggcg
    22141 cttggaaaaa ttaaaaaaaa tcgtcgccga ttcttacgaa atgacggctg aacattttaa
    22201 taatactcgc agtaaagtgg cggcgtcaga ttttcaatgg gcggccaagc aaattggtat
    22261 taatgataga gtttttgatg ccggttgtgg taatggtcgc ttattggatt atgtgcagat
    22321 tgccccgatt aattatttag gcttcgacca gagtaataaa ctattatctt ttgctcggcg
    22381 tcaacatcct ggctataaat ttgaatcagg tgatttgtct atgttaaaaa acttagagcc
    22441 agcgtctttt tctgtaattt tttgctcggc cgtacttagc cattttccca gcaagagaga
    22501 gagaattgat ttattacgct ctttttataa tctcagctct gttaaaggcc gcttgattat
    22561 cagtttttgg cgaatgaggg gtagttatct tcgtttaatt tttattaaca gattaaaaaa
    22621 aatcatcgga ttatatcctt atggctggag ggatttagtt tttttctgga aagataatca
    22681 agggcgccct attagtctgc gttattacta tatttattct cctcgtaaat ttaaaaaaga
    22741 tattttagcc gctggctgga aaatagagcg tgtttataag gactctttta attattgggt
    22801 agtggcagta aaaaatagtt gaatgaaaaa attgtcttga cacccccagg gggtgtatgt
    22861 tatactgatt ttgtatgaaa acaaccgaac agagaattaa taatattatc ggtcaacttg
    22921 aaggcataaa acgcatgctg gcgagcacgc cggaagattg ttttgctctt cttacccaga
    22981 tgaaagcagt taagtcagcc atgtgctctt tgactgagca gattttgtct tccgagtttg
    23041 accgttgtct tagtggtcgg atggcggctg acaaaagaaa gaagatggaa gtcattttta
    23101 aagaggtaat taaaaaataa aattataatt aattaaattc tgcggtccgt ttagagtaat
    23161 ggccgctaat aaatctatga gcaaagtggt ggacattgta aaaaataatt ttgaagccga
    23221 ggtcttaaaa agtgatgttc cggtattggt cgatttttgg gctccctggt gtatgccctg
    23281 ccggatgatg gctccgattc ttgacgaatt agccggtgaa cttgagggta agattaagat
    23341 tgccaaagta gatacagaaa atccagagaa tcaagagttg gcgattcaat atcagattca
    23401 gagcattccc aacatgaagt tgtttaaggg cgggcaagta gtagctgaat ttatcgggat
    23461 gcgcagccaa gatgcactta agcaagactt agaaaaatat ctttaatcaa tagaaaagcg
    23521 gccgcctttg acagcggccg ttttaatagg ttacttttaa atatatgagt atctttcaag
    23581 ataatttgga acgattacaa gccttggctt cattagacgg aattagttct gaggatttag
    23641 ccttactaag caagccagct ttggtgtcaa aaaccgagtt agaggtcggc ggtaacaaat
    23701 atccggcttg gcgtattctc tatagccatg atttaggtcc tggtaaaggt ggtattcgtt
    23761 ttcacccaga agttagtgaa gacgaagtgc agtcattggc tttttggatg acgctgaaaa
    23821 attctttagc ggatatacca tatgggggcg ctaaaggagg ggttaaattt aatcccaaac
    23881 aggtggataa aaaggaatta gaagcgatta gtcgtgctta tgctcgcgct tttcaaccgg
    23941 ttttaggcca ggataaagat gtgccggccc ccgatgttta taccaacagc cagactatgg
    24001 cttggatttt agatgaatat gaaagcattg ttggtcatca tgagcccgga atgattaccg
    24061 gcaaaccagt agaattaggc ggcctaaagt tacggtctga ctccacggct cagggcggtt
    24121 attttattgc gcaagaagtg gttaaggaat ttttaacagg caaagaagct aaagacataa
    24181 gagtggccat tcaaggtttc ggtaatgccg gtctgtttat ggcggaaaaa ctttttgttg
    24241 acggttttaa aattgtcgct atttctgata gtcgcggtgg tattttaaag gaagacggac
    24301 ttgatgtggc cgctttgtct gtttttaagg cgggaggaaa gtctgtcagt gattttgatc
    24361 agggacaagt aatcagtaat gaggaattat tagaatcaga cgttgacatt ctaattttag
    24421 ctgccctgga gaatcaaatt accgcggtaa atgccgataa aattaaagct aagcacatca
    24481 tcgaattagc taatggccca atagatttgg cggcggataa aattttagac gaggccggaa
    24541 aagtaatagt gcctgatatt ttagctaatt ccggcggtgt tattgtcagt tattttgagt
    24601 gggcgcaaaa ccgtacaggt caaattcttg atgaagatta tcttaaaaat ttattggaaa
    24661 aaaagatgag aactaactgg ggtagaattt tgtctttata taaagaaaaa aataatatta
    24721 gtttacgcca agcggcttat atcttggcgg tgagaagaat attagcggct aaacattggc
    24781 gcggtgggcg agtttaataa aagcttttta ttgataataa aagacccggg attatccggg
    24841 tcttttagtt tgttgatttt atttaattgc aggaaacatc tgtgttggtg ttgctataat
    24901 caaatccgaa gccgggtgta ggtgattgag tatttaattg ttgtccgcat tcttcagggg
    24961 cattattaaa ggcggagcag atggaggcta ataaagaggc agaatcgcga ccactctgta
    25021 cgttcgtgcc gttgataatc aaagttggtg aaccgccgac attgtatttt tcgttatcgg
    25081 ccttattaac tgcgaatccg gggaaatttc cctgccaatc cgtcttactg ttatagttgg
    25141 cagtaacctt gtattcttta tcggtagcgg ccacacagct gtctatttta tttttgtcgg
    25201 tgttcttgct taaacagtca gagccgtttc cgtctgccag gaagcattgg agataggcat
    25261 ttaatttttc cggctgttct ttttggatac aatattggac aagattttca tccaactctt
    25321 ttttttcgtg cattgcataa tcgacaaatt tcaactggaa atcaatttta tttcctaaag
    25381 tttccaaaac tggtaatatg cctttttcaa tttgagtgcc gtagggacaa tagctcatta
    25441 caaatagttc tactaccggt ttgtcatttt tagggatgtc gactgggact gtcgggttag
    25501 aagcggagtt attgttacct gcttgttctt gggcgatttt atccatatca atggcttgag
    25561 gaaaaaagag ttttccgtct ttgctgacat aggattctac cgggctttcg gagccgatat
    25621 tgattttcag tttatataaa tcatattcct ccgaggtttc aataacagac gctttacttc
    25681 cgcttggcat taagaactga tttataaaat cttcagattt agcaaccgct tccgcgatgt
    25741 ctatttcttt atatggtttc ttatcggcaa taacggtagc aataaccgct gctataacta
    25801 agattaagac ggcgataact atcgctaagg ccttcttatt tttaaaaaga cgattacata
    25861 tttttttcat atgatttttt ttattttcta taataagtaa ttataggaaa tattaaattt
    25921 aaagtcaaaa tttgtaaagt caaaattatt ttaaggtatc aataattttt tttaagtccg
    25981 atggtaggtc tatctgaaat tcttgggtgt ttccttctcg gtctttaaat tttaattttt
    26041 tagcaactaa aaatattctg cctaaagaca tttttttatt ttttaattta ttcttgtttg
    26101 ttccatacaa gtcgtccccc accaaggcgt gtccgtaggc ggcgaagtga gctctaattt
    26161 gatgagttct tcccgtttta atatttactg acactaaact gaagtggggc catttttttt
    26221 caactgtaaa caaacttaag gcctttttgg cttttagtct ggccttaata ttaccttcgt
    26281 cacgattgga aagttttttc ttggcgtgat tgcttgctgg cagggccgcc atcttgtgcc
    26341 cagatttaga tctggttagg ggaaagataa tttctccttc gtctttgata atttggccgt
    26401 gaaccaaagc aagatactct ttataaatgg tccgtttttt aaattgttgt ttaatagcaa
    26461 caaaggcttg attgtttctc gctataatca ttaaaccgct agcatcttta tctaagcggt
    26521 ggacgatgcc cgggcgggat ttgtcttccc caaccttttt aatttcaggg taagactcca
    26581 atatataatc aattagagct ttgcttttat tgccggtggc tttatgaacg atgagcccag
    26641 ccggtttgtt gattactaaa aaatctccgc tttcgtaaat tatttggatg tcaccggcgt
    26701 ggctatgaat tgttttcatg ttatttttta ttgaagatta ataatagatc gctgcgccgg
    26761 aaggctttta ataaacctaa aaacaagata taaaaaagac cggcagcagc aatagttaat
    26821 agaagcccta gggctgaatt tgtccaagtt gatggccata tccacaacaa agcgcccata
    26881 agtagggagg ctatcagggc ctttaatgtt cccctccagc atatgcggaa acctaaatgg
    26941 cgataaataa accaagttga gagaacagca atagtcgctt cgctataaat agtgaccgcc
    27001 gctgccccaa aataggagaa gcgagggata agaatgatgt aagccaccag agaggtaatg
    27061 ctagtaaata catatatcca gattactttc ttttgagctt taatggcaat aattgcgtga
    27121 gccatcatgt tgcccataaa gatggcggca atggcaaaaa ttaatatgcg tagaattata
    27181 ccagcttcta aaaagtcagg accggcgata agaacaataa ttttatcagc taagaattga
    27241 gtgccgacaa ctaaaggaat ggcggcggcc gccattatgt caaatgattt ttgtaatatt
    27301 ttataaaatt tttctttatt ctggcgccaa gtcgttatca taattggtaa aatgatgccg
    27361 gcgaacataa aaggcagcga actaagcacg tcaataattt tataggctgc gccgtagaga
    27421 ccgacagtat cggcggtctt aacaagagat agaatcagtg tatccgcgcg taaatagata
    27481 agattgaagg cgatagttat ggctagcggc caagaaagat taagaatatt ttgccacacc
    27541 ggccaattaa ataaaggctt aattttagca aagcgatggg cgaagataaa atgaaggaga
    27601 aagctagtta gactggcgga taccgtggcg atgagcatgc cgttaagccc ccaatttaga
    27661 ttaatggaaa taataacacc tgcaatcatc acgaagcggc ttagagtttc cgcccaagca
    27721 actcgattca ttttcagtct agtttggaaa agaccgacca taatctgatt taaagcgtta
    27781 aaaacaaaag aggcggctgt aatcaagacc cctaacttga tgctattatt ataattagcc
    27841 aattggacaa tgattggggc taagccaata aaaattaagg aagatattaa gcgcaaacca
    27901 aaaagatttc ccagaataag attttctttt tcttggtcgc cactaatcat ttggacggtt
    27961 accagagtta gtcccaaatc agccataata gcaaaaaagg agaggaaggt gataatggtg
    28021 gtgtattcgc caaagccatt ttgccctaat tctcttgtca ttaaggcaaa agcaaacaga
    28081 tttaaaccgg tggatgctaa tttcccgata atttgtacca aggtgttgct aaatattttt
    28141 tgctggagac tcatattttc ttgttttttc aggaaagatt ttatataatg attatagtat
    28201 aacataaatt atatgagcac taccattaag aaaactttgt ccgccttttt tcttttattt
    28261 tttggtttat tgcaaatagg cctttttggg ccgcctcagg ccgctattgc tgatagcagt
    28321 ctgattaata gtcaggaagg ttttaaagac aaggagatac aagcggcttt cggtaatgac
    28381 actcctgacg atattcgtat tacgatggtt agaatcatta tgattgtcct gtcaaccttg
    28441 ggggttgttt ttcttgcttt gttattattg gccggcttta aatatatgac ggcggctggt
    28501 gacgaagaga aggtgaaaga ggcgattaag cagattactc aagcagttat cggtctagtg
    28561 ataattttat cagcttgggg aataagttat tttattcttc tcagaattag agcggctata
    28621 actggcacca attatcttta tttctaaatt cgattact
//
LOCUS       DAOQSA010000011        28461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_1010_length_28461_cov_8.194008, whole genome shotgun sequence.
ACCESSION   DAOQSA010000011 DAOQSA010000000
VERSION     DAOQSA010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 28461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28461
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1010_length_28461_cov_8.194008"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="PKK65_01875"
     CDS             <1..1066
                     /locus_tag="PKK65_01875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HNU81463.1"
                     /translation="AWFLSIIYVSFGHLLVRWLRRLLFRYGLGTRNLVLVGNSKTTDK
                     LLSEFASTPQWGYRVVKRLRDFSLESAQELTEIMQAQRVDEVMQSDPNLSKAEALRLY
                     DFTSEHHLVYKYVADLLEVKVLRTEVSDLAGIPIVEVKKTTLDGWGRIWKRLFDIFGS
                     ALLIILFSPILLLVALAIKLDSRGPVFFSKRDDGSFVTRVGEGGRPFHYFKFRSMIVN
                     SDSMRYKELADKNLRTDGPLVKIKDDPRVTGVGKLIRRWSLDELPELFLVFIGKMSLV
                     GPRPHLPEEVAKYEHHHKKTLTVKPGITGMAQVSGRSDLLFEEEVKLDVYYLENWSLL
                     LDISILLKTPLAVLKHRQAE"
     gene            1076..2212
                     /locus_tag="PKK65_01880"
     CDS             1076..2212
                     /locus_tag="PKK65_01880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNU81464.1"
                     /translation="MKVALIHDHLAQAGGAEKVLKALTEMFPEAPIYTLLYEQKNVDK
                     NFAGKKIETSIIQRLPGGVRHYKWYLFFMPMAVEFFDLREFDLVISDASAFAKGVITS
                     PNTLHLCYCHTPTRYLWSDAHQYLNELKYNKWIKKIISLTLGRLRLWDYAAAQRVDYF
                     LANSATVQKRISKYYRRDSEIIYPPVETDNFQIADLSQQSHQERYFLSGCRLAPYKRV
                     DIVVQAFKALGDGFNLKIFGDGLDWPRLQRLAGRAANIEFLGRVSEDQKAELYAHALA
                     FINPQEEDFGITAVEAMAAGRPVLAYRRGGATETVVENESGLFFNEQTPDAIMSLVRC
                     FLEDLDSGRKVWDSRAIKAHADTYAVSEFKRRMFAFIKSKQALA"
     gene            2217..3362
                     /locus_tag="PKK65_01885"
     CDS             2217..3362
                     /locus_tag="PKK65_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNU81465.1"
                     /translation="MMRIGVDIRVLMDKQYSGVSEYSSILLRTLLRLDFDNEYRLFYN
                     SCRDVRARIGNFEHPNLLIKRSRYPNKFFNYCLQKGFAWPKLDRLVGGVDIFWSPHIN
                     FSNFSQETKHVLTIHDISFLHYPEFFSYRKNFWHRSLALKKLATRATAIVAVSENTRQ
                     DIIELLKIEPEKVQTIYSGVNPGVPMPGPEEERNFFRRHELKKRFILYLGTIEPRKNI
                     VGLIYAYNRLRDDNVNLVDLQLVLAGGSGWRNRPIYRAWEESPYRRDIKFLGYVSARE
                     KELLYRRAEVFIYPSYYEGFGLPPLEAMARNLPVITSNVSSLPEVVGSAALTINPYDI
                     NEIAKALELVVTDSQVRTRLIKAGQEQIHNFSWEKTAKQYLELFKSL"
     gene            3422..4504
                     /gene="mnmA"
                     /locus_tag="PKK65_01890"
     CDS             3422..4504
                     /gene="mnmA"
                     /locus_tag="PKK65_01890"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNU81466.1"
                     /translation="MKKKKLKILVAMSGGVDSSVAAQLLKNQGHEVAGVFLNFWKDEA
                     DPHKSANKDCSGESLRDARKVAAKIGIPFFTLDFAAEFKKVVVDYFLAEYEAGRTPNP
                     CVVCNKQIKIGLLLRQALSLGYDAVATGHYLKRLEQNGEVKIYRAKDNYKDQSYFLYT
                     FSQSEIKRLYFPLGNYTKSEVRALAAKYGLPTASKAESQDLCFLKGPHNNFLKRHLKL
                     KPGPIKRQEDGQIIGEHLGLPLYTVGQRKGIEIGGTGPYYVAEKDRKNNILWVVRTWD
                     DNLLYRREFTIKQATWMDKKRPSLPLSCRTVIRYRHDAWPCIVTAGKTRGELKVILKK
                     AIRAVTPGQSAVFYQGRRLLGGGVIQ"
     gene            complement(4560..5048)
                     /locus_tag="PKK65_01895"
     CDS             complement(4560..5048)
                     /locus_tag="PKK65_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81467.1"
                     /translation="MAFAFGAPSHTLSNQRIAQIASRKAQELHISIYTQLDVRIEPDI
                     EVRRVKENPGNPPSTWRIARGVVQLAEQQGFNEILIVAAKPHLPRALRDVERAVREAG
                     KDIKIRVCEEIEQYPEDSWFCPDSTQDRVRSKKKWDKREKIFKLMPFFIYKNIATKVM
                     RY"
     gene            complement(5231..5317)
                     /locus_tag="PKK65_01900"
     tRNA            complement(5231..5317)
                     /locus_tag="PKK65_01900"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5281..5283),aa:Leu,seq:caa)
     gene            5436..6209
                     /locus_tag="PKK65_01905"
     CDS             5436..6209
                     /locus_tag="PKK65_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNU81468.1"
                     /translation="MAKIIAIVNQKGGVGKTTTTVNLGAYLAEAGKSVLLLDLDPQAN
                     ATSGLGIEYQKLEDGIYEALLGQKSLSSVIKKTSQERYHIVPATIALAGAGIEMVSLE
                     NREFRLALLLEEIRADYDYIIIDSPPSLGLLTVNSLVAADAVLIPIQSEYYALEGLGQ
                     LLETINLVKDNLKPNLEIMGAVVTMFDRRNKLSGAVLEELNKFFPGKIFKSVIPRSVK
                     LAEAPSYGRSILHYDPKSRGARAYEELAQEVIALESKAL"
     gene            6416..7294
                     /locus_tag="PKK65_01910"
     CDS             6416..7294
                     /locus_tag="PKK65_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNU81469.1"
                     /translation="MAQALGRGLDSLIPKKNFEAVSSNNTTKEPLAVLSDTDRVFHVD
                     PDRIVMNPYQPRHNFSEITLQELMASIKEHGILQPLVVTRQGDSYELIAGERRWRSAK
                     ALGLKEVPVIIREASEQKKLELALIENGQRQDLGLIEAARAYRRLMDEFNLTQEEIAK
                     KVGKARSTVANILRLLELPEEIQHALSSGRISEAHAKYLLGITSPGQQMVLFKKIINQ
                     NLTVAQTSAETKRLGGTKAARITVNYADQDKEAKLRSFFGTKAKIIRSRHGGRVVLDF
                     YSEEELGNIIDKIENI"
     gene            7466..11122
                     /locus_tag="PKK65_01915"
     CDS             7466..11122
                     /locus_tag="PKK65_01915"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNU81470.1"
                     /translation="MFVHLHNHTHYSLLDGLTKIDELVSRAKEEGSNAVAITDHGVLY
                     GAIEFYQKCKKAGVKPIIGYEAYVAPASRHDKNTRADGRSYHLILLAKNETGYKNLIK
                     LVSLAHLEGFYYKPRIDWELLTQYHEGLIALSACLGGEISQHILNGEGEKAKKRIKEY
                     SELFGPDNYYLEIMDHPELEGQEKVNKQLIAFSKALGVPLVATNDVHYFRPEDAEAQD
                     ILLCLQNKKKIDDMDRLRMIGHGDYSMRPAVEMERAFAQVPEAIANTVKIAEMCNLNI
                     PLGEIQLPFFEVPEGYDGNSYLRFLCEKGLEKKYGLIKSGESYVLKEGSFLPGDLSLE
                     KILERLNYELSIVAQMGWSAYFLIVADFVNWAKDNKIVVGPGRGSAAGSLICYLTGIT
                     NLDPLRYDLLFERFLNLDRISMPDIDMDFADARRGDVLDYVGRKYGHDHVAQIITFGT
                     MAARAAVRDVGRVLGAPYDYCDKISKSIPMFTSLKKALAEVPELKEMYQNEPEAKRIL
                     DYAQRLEGVARHSSTHACGVLITPKPLTEYVPIQYASSADKSIISQYSLHPVEDLGLL
                     KMDFLGLKNLTIIESALRIIKKTRGLEIDIDKLPLNDESTYRLFQQGETTGVFQFESS
                     GMKRYLRELKPTEFEDIIAMVALYRPGPMEWIPDYISGKHGEKKVSYLHPKLEPILAR
                     TYGVAIYQEQVMQISRALAGFTPSEADILRKAMGKKIPALLAEQKEKFIDGCVKNGIY
                     KELAEKVFSFIEPFAGYGFNRSHAACYAMIGYQTAYLKAHWPVEFMAALLTSDQHDSD
                     RVAIEIEECRSMGIKVMPPDINESFDSFTVVTAGTKENRTAGANEKVDTIRFGLNAIK
                     NVGEHIVEVIIAERKERGPYKDIFDLLERVNDKDLNKKSLESLIKSGSLDSFGELGFL
                     LSNLERLLLFNKENSKQQESRQASLFAAAPQFINLSRPQLLPAEPIASIDRLSWEKEL
                     LGLYISDNPLSMFTQYLSGFSLPLSRLKNHRRDDYLVVAGVITSLKKIITRKGESMLF
                     AKIEDQSGSVELLVFPSLLKITSSLWRDGGLIIARGRLSEKDSDLKILVDQAGSLSPA
                     SPQSSIDEFKRTLLASKIENHWRPKNNGDWNKSQSPSFSATPLSPRRANIEIEPALRL
                     VFKQAPDAAQQAVLKELLASYPGDNPVYFRVPDGKKDNILKISATVDNSEKLRGELRA
                     TCGSFVQVVEPERA"
     gene            11251..12690
                     /locus_tag="PKK65_01920"
     CDS             11251..12690
                     /locus_tag="PKK65_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81471.1"
                     /translation="MPKLKKEADNKPALKKTRAKRVVKKEKPLPKAKPITIDVIADDE
                     DVLTRHPVEELPADNFEERESLDVQKKFFSDLVAEMKDKKQAPNLSTSLPDNKDSASR
                     SRKSLHLYRRIAFQFIALTAVFLVIVAYFFLPSLKISLHPRAEVISDSISFNIVPALD
                     KQAPAVANSRSLIGRIQSVPLSAEKVYEASGEEILGEEINGEVTLHNEYSKAQPLVAN
                     TRLLSPDNKLFRLKEAVNIPAGGKVKAQIYADKVSPEMAIGPTRFTIPGLWLGLQDQI
                     YATSDEAFEYSHKVKKYVKQRDLDQAINDIKSILAAKVDNSLASFYNDHESLAYSLDD
                     SNAKIDLGAKLGEEVAEFKVSATNNLTLVSFSKEQAETLVKAKLAFLLPDDKKLSAFD
                     SKDIAYRLDAFDVDTGTATVTASFKGLMSLRTDANIIDRRQLVNLNEAQIADYLRSFP
                     EIESYELKFFPAFIKRAPSLADRIKITVE"
     gene            complement(12776..13714)
                     /locus_tag="PKK65_01925"
     CDS             complement(12776..13714)
                     /locus_tag="PKK65_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HNU81472.1"
                     /translation="MVKFSVPLSVPKQQRDNYLKNLKLATRGCGRLFLFAGDQKVEHL
                     NADFYGPGITPEDASPAHLFAIASQIPGAVLAAHVGLLATYGKDYKNLPYILKVNGKS
                     PLSGSQDNLYSGAWLKNDHVIRFQRQSGLKIVGLGYTVYLGSNQEAQMLKEAAEMIHS
                     AHQAGLLAIIWMYPRHPQIKNEDDVHLIAGGAGVAACLGADFVKVKYPYKTANTETKA
                     AAFKEVIRAAGKTRVICVGGGRQKAEDLLKHLFRQLKNGAAGMAIGRNLHQRPLKEAM
                     SLGMAISALIHDNVSLNQALAILKKEPASLKNQKKS"
     gene            13783..14703
                     /locus_tag="PKK65_01930"
     CDS             13783..14703
                     /locus_tag="PKK65_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81473.1"
                     /translation="MNKKPRENKEADLENYEDLEGLTLKKMNIGLWLSLHRRQIMRIF
                     IIILIITAALSFAYSAYSYLYYFLYGRQADTELLHNLTDNQINSQAYRDLNAPKNLVV
                     GDISVFSVQGKYDFLVPLENPNARHYSNFRYCLENSDGVQLLCGDSFILPGAKKYLLL
                     LGQELEEKPSNLRLVVVNMNWQRLNAHDIPDWNSYFKNRADVQVLDNKYSLTGSGDKN
                     PFHSLQFTVKNNTPYHFATLPIDIILWNGARIVGVNAYNLENLLSAETRSVTLSWPSG
                     GERVNKVEIIPDVNILDQSVYFPYRGEIIP"
     gene            14707..15813
                     /gene="rodA"
                     /locus_tag="PKK65_01935"
     CDS             14707..15813
                     /gene="rodA"
                     /locus_tag="PKK65_01935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNU81474.1"
                     /translation="MWMKIRLYLKEMDWLLLAAVVLLASFGLMEIYSVALGQGSLNLL
                     NFYKQLIFVAIGLVLLFFFAFVDYRFLKSLRRYLYFFGVTVLVAVLIFGSSIRGTRGW
                     FDVAGFGIQPVEIIKIILLIFLAGYFADLATKVKTWRHLVVSGVAAAFLMFLVLMQPD
                     FGSALMLGAIWLIMVILAGFPKKYLVAIFVSSLLIFILAWSFFFKDYQRQRLLTFLNP
                     GNNSLVEGYNISQAIIAVGSGSWHGKGMGLGSQSQLKFLPEAQNDFIFSVIAEEFGFL
                     GVMVVLLLFSLFFSRCLAAVKRLPNDFSIYFVLGAAGLIFIQMFINIGMNIGIMPVVG
                     LSLPFISYGGSSIFSLFILTGIIENIIIKSKINY"
     gene            15862..16503
                     /locus_tag="PKK65_01940"
     CDS             15862..16503
                     /locus_tag="PKK65_01940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNU81475.1"
                     /translation="MQFKLSAEIREKNEKLGQGDLAAVLYGKGLESTILKLNYNTFTK
                     VFAEAGESNLITLELNKQEFPVLVKAVQKDVLKGKYLHIDFYKVDMKAKVKTEIPLEF
                     RGESRAVKELGGVFLVNINEVAVECLPSNLVDHIDVDISGLNEFGDAIYLQDLKLPEG
                     IELMHESNEVICIIEEPKRVEEEVPAAEVPATEATTAKEGEEKTEDKKTEDKK"
     gene            16522..17622
                     /locus_tag="PKK65_01945"
     CDS             16522..17622
                     /locus_tag="PKK65_01945"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HNU81476.1"
                     /translation="MEDGGRKYLSKKGRAILISALFILVFSGLGLYYLVFSPNWQWLS
                     FLDSSSSPTSTEVPLRDEAVVENDDGCVDCPISWLNGLPVANEKEASAFPVAVVIDND
                     VLARPQNALSLASLVYEAPVEGGMTRYLAIFPANIDVSAVGPVRSARPYFVAWAEELR
                     ALFIHCGGSPEALDLLKSANLYDLNEFYNGNYFWRETSVVRMAPHNVLTSGDNWRSYL
                     SQRGLDEKKADSWLFKKEVKPEKTPEPKDISLRFSANFQALWRYLPESNSYQRYFNGV
                     ESRDLAGLIQAKNVIIQKVESRILDVAGRLALDLEGEGRAEICLDGICRSGFWRHRPG
                     ERTRYYYDDGAEIQLNPGITWIEVADSNTHLE"
     gene            complement(17627..18493)
                     /locus_tag="PKK65_01950"
     CDS             complement(17627..18493)
                     /locus_tag="PKK65_01950"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HNU81477.1"
                     /translation="MNNIGQYLATAKSFSREALSLLAFVCGRDSSWIIAHPEYKLNNQ
                     ETKLFTKKFEQLKTGRPLAYLLGEKNFYKHSLIVTPAVLIPRPESEIIVETAIDYYQK
                     KRHNRQIFLDLGTGSGALIIAIADHLKSKEVSRHGRCYFGASDISPAALAIAVKNAKK
                     QHLKSQISFRSGDLLQPWRRYLQINRRSPLFIAANLPYLTPAEKARETSIAYEPKLAL
                     IGGKDGLELYRRLLQQIETLKLSGPLLLMMEINPHQAAALVKEAKKYYLPQYIQKKAD
                     LSGRTRFIIIDK"
     gene            complement(18499..19536)
                     /gene="prfA"
                     /locus_tag="PKK65_01955"
     CDS             complement(18499..19536)
                     /gene="prfA"
                     /locus_tag="PKK65_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNU81478.1"
                     /translation="MYEEIKEKFAAIEQKLSDPELIKDHQAMIKVSQEHAALKHLINL
                     INEEERLIKEIAANNNIINQETDEDLKDLAIAELSSFTEQLEKIKEEIEEECHPQGPN
                     DHKNAIIEIRAGTGGDEAALFAGDLFRMYVRFCERRGWKTEMIESSAIGSGGFKEIIF
                     AVKGQGAFGFLKYEAGTHRVQRVPETEKQGRIHTSTATVVVLPEAEEVDIEIKPTDIR
                     VDTFCASGPGGQSVNTTYSAVRILHLPTGLIVSCQDQKSQHQNKEKALQVLRSRLLAR
                     QEEERIAANSSARKNQVGAGDRSDKIRTYNFPQDRMTDHRIGQSWHNLSSLMDGDLEE
                     VIKTVKKASRE"
     gene            complement(19621..19962)
                     /gene="rpmE"
                     /locus_tag="PKK65_01960"
     CDS             complement(19621..19962)
                     /gene="rpmE"
                     /locus_tag="PKK65_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNU81479.1"
                     /translation="MKKDIHPQYHKNCVVTCVCGNSFETGSTVPEIKVELCSACHPFY
                     TGKQKLVDTARRVEKFSARVAAKTSIGAGKKGKKVKKAARAEAKANKEVVVKTKDIKK
                     IAQVTLNAKKK"
     gene            20131..21561
                     /locus_tag="PKK65_01965"
     CDS             20131..21561
                     /locus_tag="PKK65_01965"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HNU81480.1"
                     /translation="MNKVANIAKNTSYLTLALIIQKFISFFYFILLARFLGLHALGQY
                     YTAISFTTVFAILIDCGFNNVLIREVAKDQSKASDWLQSVLSIKLWLALGTFIFALIV
                     ASFAYDQTLFVLIIISACSMVLDAFTSTFFSVARGFHNLKYESIASVVFQLIVLIFGY
                     LALLASWPLWLLLSALVAASLFNFTYSAFIIKRQFKLTIRPRWDAAKVKRIFLISWPF
                     ALYNALQRLFTYLDSLLLGFIAGYAQVGLYQIAFKLIFALQFLPMAFTASLYPAMSTY
                     WQDNREQLRISFERAINYLIIISLPIIFGVFALADKIVPIFKAGPEAIWPLRLSIVAL
                     FFIFLNFPVGSLLNACDRQRRNTANMAIATVISVAINLFLIPRWGALGASFTVLFSNA
                     LMLSLGAWSMRGLIVYRPRQNFKILWRSFLAAALMAVIIWQGKAYIHVFLAIAGGGVV
                     YFLALMALGALKKADINSVINSFRRS"
     gene            21565..22650
                     /locus_tag="PKK65_01970"
     CDS             21565..22650
                     /locus_tag="PKK65_01970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNU81481.1"
                     /translation="MKILLATLEYPPQIGGVASYYGNLIRHWSPDDVWQVVDNSQNLL
                     LASRGILPWRRALLTLRRAINKNKPDFLFLGQILPLGTVAWVLHYFKPLPYGIFLHGM
                     DLSFALRSRRKRLLTRLILRKARIIVCANSRVQKILLNFYPAAAAKVVLINPGASAGK
                     PVEEIKDRLIQEYGLADKKIIFSMGRLVKRKGFDQVIAALAKVPGENWRYLLAGDGPE
                     REALQALATASPQSQKIIFLGSLGEEEKWSCLDLCDIFIMTSRDLAGDFEGFGIVYLE
                     ANLMGRPVIAGNSGGVGDAVINMVNGLVVDSENQEEIAEAINQLLASDDLKKKLGRQG
                     KVRAEVEFTWSKQAETLRQYLSSLKNL"
     gene            22647..23348
                     /locus_tag="PKK65_01975"
     CDS             22647..23348
                     /locus_tag="PKK65_01975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNU81482.1"
                     /translation="MISIIIPVYNQAKKLSLTLASIARQSLSDWEVIVVNDGSSDNPE
                     AVFADFCAQIKIDNRFIFLSQENQGAAAARNRGYQEARGEYLFFCDADADLQPDALEI
                     MLQALVDNPAASYAYPSFFWGKKLFKVGPFNAARLQAGPYIHTMALIRRSDFPVNGWD
                     ESLRKFQDWDLWLTMMEAGKQGVWIDKVLFKITPGGSISSWLPSFAYKLLPFLPAVKK
                     YRRAMAIIKKKHVLA"
     gene            23371..23802
                     /locus_tag="PKK65_01980"
     CDS             23371..23802
                     /locus_tag="PKK65_01980"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNU81483.1"
                     /translation="MFIALLLLVLLAAFLIWRFFVPNKYDYLPVQAGDCIFMVETALN
                     PAQQYQGLSHRTSLDADKGMLFLFSPAADKTFVMRKMNFPLDLIFIRNHRIVNLYHEA
                     APEGKSPETSYHSGVPVDAVLEIKAGRSRDCHLGVGSEITW"
     gene            23806..25275
                     /locus_tag="PKK65_01985"
     CDS             23806..25275
                     /locus_tag="PKK65_01985"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNU81484.1"
                     /translation="MNLFKVKSPYFFIFSLIILVELLSFTAYFLPQLQIYLLITAFLA
                     VFFLSLSSLESGLLVALAELVIGSKGHLFSASIFGVSVSLRLVIWLALMLASCIFIFR
                     RGFPTVWRTKLFNFSYWPWVSVLAAFIILAFVHGYLRGNGASDIFTDGNAWFYLGLLL
                     PTILAYSQIDDKQEQRLRAVFFTAIFWLSLKTLMLLFIFSHNISIMPDVYLWIRRSGV
                     GEITAMGGGWYRIFIQSQIYIPIAFFLLLWPSLDLKTKKNPYLLIISLAVLLSVIIIS
                     MSRSFWLAFAFTAGVMAVISFRKKISLYLKALVYTGAALGLSLLLILVVVKFPYPNPQ
                     TGLSAAALSARLDFSSGEAAISSRWSLLPNLWQSINDSLFWGKGFGASVSYFSQDPRV
                     LSLNPSGWYTTYAFEWAYLDTWLKLGLFGLIAYFAWLIVLIVDLLKKMREHGKMLPAA
                     ILAGLIFLMVVNIFTPYLNHPLGFGFLIFSSCFIKKNPL"
     gene            25339..25797
                     /gene="greA"
                     /locus_tag="PKK65_01990"
     CDS             25339..25797
                     /gene="greA"
                     /locus_tag="PKK65_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012772085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNU81485.1"
                     /translation="MSEQIITQEGYDKLQDELNYLSLIKRREIAERIERAKDLGDLSE
                     NAEYSEAKDAQALNEGRILEITNILKNVTVVNNEGGHEDVTMGSCVTVKCDGKEKKYT
                     IVSFNEADPINGKISNESPLGVAFLGKKKGAIVQVETPRGIVEYKIVKID"
     gene            25803..27317
                     /gene="lysS"
                     /locus_tag="PKK65_01995"
     CDS             25803..27317
                     /gene="lysS"
                     /locus_tag="PKK65_01995"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNU81486.1"
                     /translation="MSAEKNLAPGSEREERLKKLEALKAAGINPYPAKSQRNYSIAQI
                     FSDMEKLEANQTKVIIAGRLREVRGHGNLTFAHLEDASGRIQLAFSQKELGHESYKRL
                     VKLIDSGDFLETAGTIFVTHSGEKSLMVSDWKVLSKAIAPIPNAWYGLKDEDERYRHR
                     YLDILLNPEIKDIFYKKAKFWEVTRDFMKRHGFLEVETPTLETTTGGAEANPFRTHHD
                     DFDLDVFLRISVGELWQKRLMAAGFEKVFEIGRVYRNEGSSPDHLQEFTNMEFYWAYA
                     NYEMGMKLTQELYQEIALKVFGRTKFSTKGMEYDLAGDWPKLDYRETVLEKTGIDILK
                     ASEEEIKSKLDELQVKYEGNNRERLMDTLWKYCRKSMAGPVFLVNHPKLVSPLSKAKP
                     ENPELTERFQIIIAGSEVGNGFSELNDPIDQRQRFELQQKLIDRGDKEAMMPDWEFVE
                     MLEHGMPPTCGFGFGERLFAFMVDKPVRETTLFPLMRPKREEMEKKDSPLKKNK"
     gene            27372..27445
                     /locus_tag="PKK65_02000"
     tRNA            27372..27445
                     /locus_tag="PKK65_02000"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27404..27406,aa:Gln,seq:ctg)
     regulatory      27643..27749
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            28101..28391
                     /locus_tag="PKK65_02005"
     CDS             28101..28391
                     /locus_tag="PKK65_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81487.1"
                     /translation="MKNENTIKIGKTFTTDGHREEKEIYCLEDGEERFYNILGTGYGI
                     NLVHCFSMGAKNKENFWERVFAYKIIPNREPANLMKRVTGKDYISVEFYELS"
BASE COUNT         7654 a         5382 c         6534 g         8891 t
ORIGIN      
        1 cgcttggttt ttatcaatta tttatgtgag cttcggtcat ttgctcgtgc gctggctgcg
       61 gcgtttgctt tttcgctatg gcctcggcac gcgcaacctg gtcttggttg gcaacagcaa
      121 gaccacggat aagctgcttt ccgaatttgc ttccactcct cagtggggct atcgcgtcgt
      181 caagcgttta cgtgattttt ccttggaaag cgcgcaggaa ttaacggaaa taatgcaagc
      241 ccaaagagtg gatgaggtca tgcagagcga ccctaattta agtaaagcgg aagcgctgcg
      301 cttgtatgat tttacctccg aacatcatct ggtctataaa tacgtcgccg atttattgga
      361 ggtaaaagtt ctgcgtaccg aggtcagcga tttggccggc attccgattg tggaggtgaa
      421 aaaaacaact ttggacggct ggggacgcat ttggaaaaga ttatttgata ttttcggttc
      481 cgctctttta attattctgt tttctccaat tttactatta gttgctttgg ctattaagct
      541 cgattcccgc ggtccggttt tcttttccaa gcgcgacgac ggctctttcg ttaccagagt
      601 cggcgaaggc ggtcggcctt tccactattt taaattccgt tccatgattg ttaattccga
      661 cagcatgcgc tacaaggaat tggctgacaa aaatttacgt accgacggcc ccttggtgaa
      721 gattaaggat gatccgcggg tgactggcgt tggtaagctt atccgtcgct ggtctttgga
      781 cgaattgccg gaattatttt tggtgtttat cggcaaaatg agcttggtgg gcccgcgtcc
      841 gcatttaccg gaagaagtgg ctaagtatga gcatcatcat aaaaaaaccc taacggttaa
      901 gcctggtatt accgggatgg cccaagtttc cggtcgcagc gatttgctgt ttgaagaaga
      961 ggtgaagctg gatgtatatt atttggaaaa ttggtctttg cttttggata tttctatcct
     1021 tttaaaaacc cctttagcgg tcttaaaaca tcgccaagcg gaataacata aaaagatgaa
     1081 agtcgctctc atccacgacc acctggccca agcgggcggg gcggagaaag tgttaaaggc
     1141 gctgacggaa atgtttccgg aagcgccgat ttacacttta ctgtacgaac aaaagaatgt
     1201 tgacaaaaat tttgccggca aaaagataga aacttcgatt attcagcgct tgcccggagg
     1261 ggttaggcac tataagtggt atttattttt catgccgatg gcggttgaat tttttgattt
     1321 gcgcgaattt gatttggtaa tttctgatgc cagcgctttt gctaaagggg tgattacttc
     1381 gcccaatact ctccatctct gctattgcca cacgccgacg cgctatcttt ggagcgatgc
     1441 ccatcagtat ttaaatgaat taaaatacaa taagtggata aagaagataa tttctttaac
     1501 tttagggcgt ctgcgtctgt gggattacgc cgccgcgcag agagtcgatt atttccttgc
     1561 caattccgcc actgttcaaa aacgcatcag taaatattat cgtcgtgatt cggaaatcat
     1621 ctatccgccg gttgagaccg ataactttca aattgccgat ttaagccagc aaagccatca
     1681 agaaagatat tttctttcgg gttgccgttt ggcgccgtat aaacgcgttg atattgtggt
     1741 tcaggctttt aaggctctgg gtgatggttt taatttaaaa atttttggcg atggtctaga
     1801 ttggccgcgt ttgcagcgtc tcgccggaag agcggccaat attgaatttt tagggcgcgt
     1861 tagtgaagac cagaaggctg agctctatgc ccacgccttg gctttcatta atccccaaga
     1921 agaagatttt ggcattaccg ccgtggaagc gatggcggcc ggccggccgg ttttggctta
     1981 tcgccgcggc ggcgctaccg aaactgtcgt tgaaaatgaa agcggcctat tctttaacga
     2041 gcagacgcca gacgctatta tgtctttggt ccgatgtttc ttggaagatt tggatagcgg
     2101 ccgaaaagtt tgggatagcc gggcgattaa agcccacgcc gatacttatg ccgtttctga
     2161 attcaagcgc cgaatgtttg cttttatcaa atctaaacaa gctttagcct aattatatga
     2221 tgagaattgg cgtcgatatc cgcgttttga tggataagca atatagcggc gtttccgagt
     2281 atagttcaat cttgttgcgc acactcttgc gtcttgattt tgataacgaa tatcgtcttt
     2341 tttataattc ttgccgtgac gtcagggcgc gtatcggtaa ttttgagcac ccaaatttgc
     2401 tgataaagcg gagccgttat cctaataaat tttttaatta ttgtttacag aaagggtttg
     2461 cttggccgaa acttgaccgt ttagtcggcg gggttgatat tttttggtcg ccgcatatta
     2521 atttcagtaa tttttctcaa gagactaaac atgttttgac tattcatgat atttcctttt
     2581 tacattatcc tgaatttttt agctaccgca aaaatttttg gcaccgatct ttggctttaa
     2641 aaaaattagc cactcgcgcc acggcgattg tggcagtttc tgaaaatacc cgtcaagata
     2701 taattgaatt gcttaaaatt gagccagaaa aagtccagac gatttattct ggagttaatc
     2761 cgggcgtacc gatgccggga ccggaagaag agcgtaattt tttccgccgc catgagttaa
     2821 aaaagagatt tattttatat ttaggcacaa ttgagcctcg taagaatatt gtcgggctga
     2881 tttacgccta taatcgcttg cgggacgata atgttaattt agttgatttg caactagttt
     2941 tggctggggg cagcggttgg cgcaaccgtc ctatctatcg cgcttgggag gaatctccct
     3001 atcgccgtga tattaagttt ttaggatatg tctcagcccg cgaaaaggaa cttttatatc
     3061 gaagggcaga agtttttatt tatccttcat attatgaagg ttttggcttg ccaccgcttg
     3121 aagcgatggc gcgcaatttg ccggtgataa ctagtaatgt ttctagtctg ccagaagtcg
     3181 ttggctcggc cgctttaacc attaatccct atgatattaa tgaaattgcc aaagcgctgg
     3241 aattggtggt tactgatagc caggtaagga cgcgattaat taaagccgga caagagcaga
     3301 ttcataattt ttcttgggaa aagacggcaa agcagtattt agaattattc aaaagtctat
     3361 aagagtaaag gagataagtc ggattgaggc cggttttttt aattttagtg acggcaaaag
     3421 tatgaaaaaa aagaaactta aaatattggt agcgatgtcg ggcggagtgg attcttcggt
     3481 agcggcccag ctattaaaga accagggaca tgaggtcgcc ggtgtttttt taaatttttg
     3541 gaaggatgaa gccgatcctc ataagagtgc aaacaaagat tgctctgggg aatctttgcg
     3601 tgatgctaga aaagtagcgg ctaaaatcgg tattcctttt tttaccttag attttgctgc
     3661 tgaatttaag aaagtggtcg ttgattattt cctggctgaa tatgaagctg gtcgcacgcc
     3721 caatccctgt gtcgtctgta ataagcagat taaaattggt ttgctgcttc gccaagcttt
     3781 gtctttaggc tatgacgctg ttgctaccgg ccattatctc aaacgtcttg agcagaacgg
     3841 ggaggtaaaa atatacagag ccaaggataa ttataaggat caatcatatt ttttatatac
     3901 ttttagccag tcagagatta aaagactgta ttttcctttg ggtaattata ctaagtcgga
     3961 agtgcgcgcc ttggccgcca aatatggttt gccaactgcc agcaaggcgg aaagtcagga
     4021 cctttgtttt cttaagggtc cgcataataa ttttttaaaa agacatctaa aattaaagcc
     4081 ggggccaatt aaaagacaag aggatggtca aattattggc gaacatctcg gtcttcctct
     4141 ttataccgtc gggcaaagaa aggggattga aatcggcggc accggccctt attacgtggc
     4201 ggaaaaagat agaaaaaata atattttatg ggtggtgcgt acttgggatg acaatctctt
     4261 gtatcgtcga gaatttacca tcaaacaggc aacctggatg gataaaaaac ggccatcatt
     4321 acctttatct tgccgcacgg ttatccgtta tcgccacgat gcttggccgt gcatagtgac
     4381 agctggaaag acaagaggtg aattaaaggt tatacttaaa aaagcgatta gagcggtaac
     4441 accggggcag agcgctgttt tttatcaagg tcggcgtctt ttggggggcg gtgtgattca
     4501 gtaatttatt ctctttagaa aaagaaaagc cccaggtggt aaaaccacaa ggggctcgat
     4561 taatatcgca tcactttcgt ggcgatattt ttgtaaatga agaagggcat aagcttaaag
     4621 attttttctc gcttatccca ttttttcttt gatctaactc ggtcttgcgt ggaatcgggg
     4681 cagaaccacg aatcttcagg atactgctcg atttcttcgc agacacgaat tttaatatcc
     4741 ttcccagctt cgcgaactgc ccgctcgaca tctcggagag cacgggggag atggggttta
     4801 gcggcaacaa ttaaaatttc attgaatcct tgttgctccg ctaattgaac gactcctcgc
     4861 gcaatccgcc aagtcgacgg tggatttcct gggttttcct taacacgtct cacctcaatg
     4921 tcgggttcga tacgaacatc gagttgggta taaatagaaa tatgaagctc ttgtgccttt
     4981 cttgaagcga tctgagcgat gcgctggttg gaaagggtgt gagaaggagc cccgaatgcg
     5041 aaagccacaa tcacggtttc caaggttaac ctccttaatt aaattgttaa aagaattaat
     5101 tataataaca taaattatac aattagtaaa tagcagaaaa accagcattt ttgctgattt
     5161 tactagtctt gctactgaat tttgtaagga aaattttgcg ttttcccact tgaaattttt
     5221 agcgaaattt tggtgcccag agcgggaatc gaacccgcat ggcctctcgg ccgcgcgatt
     5281 ttgaatcgcg ttcgtctacc agttccgaca cctgggcgat tatggactta agtataataa
     5341 ataaatattt gattgtcaaa ggctttgttt tacgatataa taaaaaagaa ctaatcgata
     5401 ttttctcttt ttttactaaa attgctgttt ttagtatggc caagattatt gctatcgtca
     5461 atcaaaaagg aggggtgggt aaaaccacca ccactgttaa tttaggagcc tatttagcgg
     5521 aagccggaaa atctgttttg ttgcttgatt tagatcctca ggctaatgcc acttctggtc
     5581 tcggcattga gtatcagaaa cttgaagacg gtatatacga agcgctgtta gggcagaaga
     5641 gtttaagttc cgtgattaaa aaaacctctc aagagcgcta tcatatcgtt ccggccacca
     5701 ttgctttagc cggcgccggt attgagatgg tgtctttgga aaatcgcgaa ttccgtttgg
     5761 ctttgcttct ggaagaaatt agagcagatt atgattatat aatcatcgat agtccgccct
     5821 ctctggggtt attaaccgtt aatagcctgg tcgccgctga tgccgttttg attcctattc
     5881 aaagtgaata ctatgcctta gaaggattgg gccagcttct ggaaacaatt aatttggtta
     5941 aagacaattt aaagcctaac ttagagatca tgggggcggt tgtcaccatg tttgatcggc
     6001 gcaataaatt gtcaggcgcg gtcttagaag aattgaataa gtttttcccc ggtaaaattt
     6061 tcaaatcagt aattccgaga agtgttaagt tagccgaggc gccgagttac ggccgcagta
     6121 ttcttcatta tgacccgaaa tcaagaggag ctagggctta tgaggaattg gcgcaagagg
     6181 taattgctct ggagtctaag gcgctttaat tataaaagac attatcatcg ccgccgctaa
     6241 caaggggaaa actcaagaaa tttccgagat ttttaagaat tctggattta acctgcatcg
     6301 gctccgattg ataattttat tgatccggtc gaagctagct gggcgggtag aatcatagat
     6361 aggcatttaa tcaagcttta caaatattaa tatctagata ttcaatataa atcatatggc
     6421 gcaagctttg ggtcggggcc tagactccct tattcctaaa aaaaatttcg aggcggtttc
     6481 ttctaataat acaaccaagg aaccgctggc ggtattgtcc gacactgatc gcgtttttca
     6541 tgttgacccg gatagaatcg tcatgaatcc ctatcagcct cgtcataatt ttagtgagat
     6601 tactttacag gaactaatgg cttcaattaa agaacacggc attcttcagc ccttggtggt
     6661 tacacgccaa ggcgatagtt atgaactgat tgccggagaa aggcgctggc gtagcgccaa
     6721 agctttaggc ttaaaagaag taccggtaat aattagggaa gcgagcgaac aaaaaaagtt
     6781 agagttagct ttgattgaaa atgggcagcg tcaagacttg ggtttaattg aagccgcccg
     6841 cgcctatcgt cgcttaatgg atgaatttaa cctcacccag gaagaaatcg ctaaaaaggt
     6901 cggcaaggct cgttctactg tcgccaatat tttacgttta ttggaattac cggaagaaat
     6961 ccagcacgct ttaagctccg gacgcatcag tgaggcgcat gccaagtatt tattgggtat
     7021 taccagtcct ggccaacaga tggttttatt taagaaaata atcaatcaaa atttgacagt
     7081 tgctcagacc agcgccgaaa cgaaacgctt aggcggtacc aaagctgccc gtattactgt
     7141 taattatgcc gatcaagata aagaggccaa gttgcgttct ttttttggaa ctaaagctaa
     7201 aattattcgt tcccgccacg gcggacgggt ggtattggat ttctatagtg aagaagaatt
     7261 agggaacatt attgacaaaa tagaaaatat ataatataat cagttaattt ttaaattact
     7321 cagtaaataa aaaatttaaa actagagacc gaaatatcgg cctcttttta ttttttaaaa
     7381 caagatttta cttctttgac aagattgctg gctccgctta aaattgctat agtttaaaga
     7441 taaaaaaggc ttaatttttg tttaaatgtt tgtccatctc cacaatcaca cccattattc
     7501 cttattagac ggtttaacta agattgatga attagttagc cgcgccaagg aagaaggttc
     7561 taatgccgtt gccatcactg accacggtgt gctttatggc gccattgaat tttatcagaa
     7621 gtgcaagaaa gcgggggtga agccaattat cggttatgaa gcctacgtgg cgccggcttc
     7681 ccggcatgat aaaaatacgc gcgccgatgg ccgcagttat catctaatct tgctggctaa
     7741 aaatgagacc ggttacaaga atttaatcaa attggtatcc ttggctcatt tggagggttt
     7801 ttactacaag ccgcgcattg attgggaact tctcacccaa tatcatgagg gcttgatcgc
     7861 cctcagcgct tgtttgggcg gggaaatttc tcagcatatt ttaaacggag agggggaaaa
     7921 ggcaaaaaaa agaattaagg aatatagtga attatttgga ccagataatt attatctaga
     7981 aataatggat catcccgagc tcgaggggca ggaaaaagtt aataagcaat taatcgcttt
     8041 ttccaaagcc ctgggcgtgc ctttagtggc gactaatgat gttcattatt ttagaccgga
     8101 ggatgccgaa gcgcaggata ttttactttg cctgcaaaac aagaaaaaaa ttgatgatat
     8161 ggaccgcctg cggatgattg gccacggcga ttattcgatg cggccggcgg tagaaatgga
     8221 aagggccttt gcccaggtgc cggaagcaat tgccaatact gtgaaaattg cggaaatgtg
     8281 caatttgaac atacctttag gagaaattca gcttcctttt tttgaagtgc ccgaggggta
     8341 tgatggtaat tcctatttac gttttctctg cgaaaagggc ttagaaaaaa aatatggatt
     8401 aataaaatca ggtgaatcat acgttttgaa agagggctct tttttaccgg gggatttaag
     8461 cttggagaag atattggagc gattaaatta tgaattatca attgtcgccc aaatgggttg
     8521 gtccgcctac tttttaatcg tggctgattt cgtcaactgg gcgaaagata acaagattgt
     8581 tgtcggtccc ggtcgcggca gcgccgccgg ttctttgatt tgctatctta ccggtattac
     8641 caatcttgat cctttgcgct atgatttgtt atttgagcgt tttttaaatc tggaccgtat
     8701 ttccatgcct gatattgata tggattttgc tgatgcccgt cgcggtgatg ttttagatta
     8761 tgtcggccgg aaatatggac atgaccacgt ggctcagatt attactttcg ggacgatggc
     8821 ggctagagcg gcggtgcgcg atgtcggccg ggtgctgggt gctccttatg attactgcga
     8881 taaaatctcc aagagcattc cgatgttcac ttctttaaag aaagctttgg cggaagtgcc
     8941 ggagctgaaa gaaatgtatc agaatgagcc tgaggcaaag cgtattttgg attacgccca
     9001 gcgcttggaa ggggtggctc gtcatagctc cactcatgct tgcggcgttt tgattacccc
     9061 gaaaccgcta accgaatacg tgcccatcca atatgcatct tcggctgaca agagtattat
     9121 ttctcaatat tccttgcatc cggtggagga tttaggctta ttgaaaatgg actttttagg
     9181 tttgaaaaac ctgactatta ttgaatcagc tttaaggatt atcaaaaaaa ccagaggctt
     9241 ggaaatagat attgataaat tgccgctgaa tgacgaatct acctaccgtc ttttccaaca
     9301 aggcgaaaca accggcgtgt tccagtttga atcgtcaggt atgaaacgct atttgcgcga
     9361 gttgaaaccg actgaatttg aagatatcat cgcgatggtg gctctttatc gtcccggacc
     9421 gatggagtgg attcctgatt atattagcgg caagcatgga gagaagaaag tctcatatct
     9481 tcaccccaaa ttggagccga ttttggccag gacttacggg gtggctatct atcaggaaca
     9541 ggtgatgcag atttctcggg ctttggccgg ttttactccg agtgaagccg atatcttgcg
     9601 caaggctatg gggaaaaaga ttcccgctct tctcgctgaa caaaaagaga aatttattga
     9661 cggttgtgtt aaaaatggaa tttataaaga actggcggag aaggtatttt cttttattga
     9721 accttttgcc ggctatggtt ttaaccgcag tcacgccgct tgttacgcga tgattggtta
     9781 tcagaccgct tatttgaaag ctcattggcc ggtggaattt atggcggccc tgcttacctc
     9841 cgaccagcat gatagtgatc gggtagcaat tgaaattgaa gaatgtcgga gtatgggcat
     9901 taaggtaatg ccccctgata tcaatgaatc atttgattct tttacagtgg ttactgccgg
     9961 cactaaagag aataggaccg cgggcgccaa tgaaaaagta gataccatca ggtttggtct
    10021 taatgcgatt aaaaacgtgg gcgaacatat tgttgaggtt attattgctg agcgcaaaga
    10081 gcgtggcccc tataaggata tttttgattt gttggaaagg gttaatgata aagatctaaa
    10141 caagaagtct ctggagagtt tgattaaaag cggttcttta gatagctttg gcgaattagg
    10201 atttctgtta tctaatttgg aaagattgct tctgtttaac aaggaaaaca gcaaacagca
    10261 agaaagccgc caagccagtc tgtttgcggc cgcgccccag tttattaatc tctctaggcc
    10321 tcaattactg ccagcagaac ctattgcctc aattgatagg ctctcttggg aaaaagaact
    10381 actcggtctt tatatcagtg ataatccctt gtcaatgttt acccaatatc tgtccggctt
    10441 tagcctgcct ttatcgcgct taaaaaatca tcgcagagat gattatttgg tggtggcggg
    10501 agtgattact agtttaaaaa aaataattac ccgcaagggt gaatcaatgt tgtttgcgaa
    10561 gatagaagat caaagtggta gtgtggaatt attggtattt cctagtcttt taaaaatcac
    10621 gagcagtttg tggcgggatg gtggtctgat tattgcgcgg ggacgtttgt cggaaaaaga
    10681 ttcagattta aaaatattag tcgatcaggc cggatctctg tcgcccgctt cgccgcaatc
    10741 ttcaattgat gaatttaaga gaaccttatt ggcgtctaaa atagaaaatc attggcgccc
    10801 caaaaataac ggcgattgga ataaaagtca gtcaccgtct ttttccgcta cccccctatc
    10861 gcccaggcgc gccaacattg agattgaacc agctcttcgt ttggttttta agcaggcgcc
    10921 tgatgccgct cagcaagcag tgcttaaaga attattagcc agttatcctg gcgataaccc
    10981 tgtatatttt cgcgtccccg atggtaaaaa agataatatt ttaaaaattt ctgccaccgt
    11041 tgataattca gaaaaattac gcggggaact aagagcgact tgtggttctt ttgtccaagt
    11101 ggtggagccg gaaagagcgt gaaacggcaa tcttattttc cctggattgt gagctcaaaa
    11161 aaaatttgat ataataaaaa atagagaatt gtcttgaatt aaagcggtat cattattttt
    11221 aaaaccggct tgtccggagg aaacaagctt atgcctaaat taaaaaaaga agccgataac
    11281 aaaccggccc ttaaaaaaac gcgtgccaag cgcgttgtta aaaaagagaa gcctttgcct
    11341 aaagctaagc ccatcacgat tgatgtcatt gccgatgatg aagatgtttt aactcggcat
    11401 ccagtggaag aattgccagc ggataatttt gaagagaggg agagtctgga tgttcagaaa
    11461 aaattctttt ctgatttagt ggccgagatg aaagataaaa aacaagcccc aaacttatct
    11521 acttctttac ctgataataa agactcggcc agcagaagca gaaaatcttt acatctttat
    11581 cgtcgtatcg cttttcagtt tatcgctttg actgcagttt ttctagtaat tgtggcctat
    11641 ttttttcttc ccagtttaaa aatttcctta cacccccgcg cggaagtcat ctctgattca
    11701 atctccttta acattgttcc cgctcttgat aagcaagccc cggctgtcgc taacagccgc
    11761 agcttaattg gtcgtattca gagtgttccc ttgagcgcgg aaaaagtata tgaagctagt
    11821 ggtgaggaga tattaggcga ggaaattaac ggggaagtaa ctttacataa tgaatatagt
    11881 aaagctcagc ctttagttgc taatactcgt ctgcttagtc ctgataataa gctttttcgt
    11941 ttaaaagaag cggtaaatat tccggccggt ggtaaggtca aggcacaaat ttacgcggac
    12001 aaggtgtcgc cggaaatggc tatcggaccg acccgattca ctattcccgg tctttggcta
    12061 ggcttgcaag atcaaattta tgctaccagt gatgaggctt ttgagtatag ccataaagtc
    12121 aaaaaatatg tgaagcagcg cgatcttgat caggctatta atgatattaa atctatttta
    12181 gcggctaaag tagataatag cctcgcttct ttttacaacg atcacgaatc tctagcttat
    12241 tctttagatg atagtaatgc gaaaattgat ttgggcgcta aattagggga agaagtggcg
    12301 gaatttaagg tttccgctac caacaacctt accctcgttt ctttttccaa agagcaggca
    12361 gaaacactgg taaaagcaaa gttagctttc ttattgccag atgataaaaa actttccgct
    12421 tttgatagta aggatattgc ttatcgcctt gatgcttttg atgttgacac cgggacagcg
    12481 acggttaccg ccagttttaa gggcttgatg tctttacgga ctgacgccaa tatcattgat
    12541 cgccgccagt tagttaattt gaatgaagcg caaattgctg attatcttcg ttctttcccc
    12601 gaaattgaat cttatgaact taaatttttt ccggctttta ttaaacgcgc ccctagtttg
    12661 gctgacagaa ttaaaatcac cgttgaataa ggggttttga tatttttttc caattaaaaa
    12721 agacgctttt actaagagcg tctttttttg atagacttaa aacagtccta aaattttatg
    12781 atttcttttg atttttaaga gacgctggct ctttttttag tatggctaaa gcttgattta
    12841 agctaacatt atcatgaatc agagcactaa tagccatgcc taaagacatt gcttctttta
    12901 gcggtcgttg atggagattg cgtccgatag ccatgccggc agcgccgttt ttaagttgtc
    12961 taaaaagatg ctttaataaa tcttccgctt tttgtcggcc accaccgacg caaattactc
    13021 ttgtttttcc ggcggccctt atcacctctt taaaagcagc tgctttagtc tcggtgttag
    13081 cggtcttata tggatattta accttaacaa aatccgcccc cagacaagcg gccacacccg
    13141 cgccgccagc gattagatgg acgtcatctt catttttgat ttgcggatga cgaggataca
    13201 tccagatgat tgccaataaa ccggcttggt gagcactgtg aatcatttct gccgcttctt
    13261 taagcatttg cgcttcctga ttgctgccga gatagacagt gtagcctaaa cccacgatct
    13321 tcaagccact ttgtctttga aaacggataa catgatcgtt cttgagccaa gcgccgctgt
    13381 atagattatc ttggctgccg gagagcggac ttttgccgtt gactttgagg atatagggga
    13441 ggtttttata gtcttttccg taagtggcaa gcaagccgac atgagccgct aaaaccgccc
    13501 caggaatttg gctggcaatg gcaaagagat gtgctgggga agcatcttca ggagtaatgc
    13561 ctggaccata gaagtcggcg tttaaatgtt cgactttctg gtcgccggca aaaagaaaaa
    13621 ggcggccaca gccacgggta gcgagtttaa gattttttag ataattatct cgctgttgtt
    13681 tggggacgga taagggaaca ctaaatttaa ccatatgatt tttttattta aaaactaaaa
    13741 tgattatgct ataatcaaaa tattcttaat tataacacat ttatgaacaa gaagcctaga
    13801 gagaataaag aagctgattt agaaaattat gaagatttag agggtttgac tttgaaaaag
    13861 atgaatatag gtttatggtt gtcgcttcat cgccgccaaa taatgcgtat cttcataatt
    13921 attttaataa taacggcggc tctttctttc gcttattctg cctatagtta tctttattat
    13981 tttctgtacg gtcgccaggc ggacacggaa ttgttgcata atttaacgga caatcaaatt
    14041 aatagtcagg cctatcgtga tcttaatgcc cccaaaaatc tcgttgtcgg cgacatttct
    14101 gttttttctg ttcaaggaaa atatgatttt ttagtaccgc tggaaaatcc caatgctcgc
    14161 cattatagta attttcgtta ttgcctagaa aatagtgacg gcgtccagtt actttgcggc
    14221 gattccttta ttttacccgg tgccaagaaa tatcttttgt tgctcggtca agaactggag
    14281 gaaaagccaa gcaatcttcg tttggttgtg gttaacatga attggcagcg tttaaatgcc
    14341 catgatattc ccgattggaa tagttatttt aaaaaccggg ctgatgtgca ggtgttagat
    14401 aataaatata gccttaccgg gagcggcgat aaaaatcctt ttcactccct acaatttact
    14461 gttaaaaata acactccgta tcattttgcc accttgccaa ttgatattat tttgtggaac
    14521 ggtgcgcgga tagtcggggt gaacgcttat aatttagaaa atttgttgtc cgcagaaaca
    14581 cgttccgtca ctttatcctg gccatcaggg ggtgagcgcg ttaataaagt ggaaattatc
    14641 cccgatgtta atattttaga tcaaagcgtc tattttcctt accgcggcga gattattccc
    14701 taatgtatgt ggatgaaaat cagactatat ttaaaagaaa tggattggct tttgctggcg
    14761 gcggtagtgt tgctggcttc tttcggttta atggaaatat atagcgtcgc tttgggtcaa
    14821 ggcagtctca atcttcttaa tttttataag cagctaattt ttgtcgccat cggtttggtg
    14881 cttttgtttt tttttgcttt tgtcgattat cgttttttaa aaagcctgcg ccgctatctc
    14941 tattttttcg gggtgacagt tttggtggcg gttttgattt tcggttccag tattcgcggt
    15001 actcgcggtt ggtttgatgt ggccggcttc ggtattcagc cggtagaaat tatcaaaatc
    15061 atcctcctta tttttttagc cggttatttt gccgacttgg cgaccaaagt taagacctgg
    15121 cggcatttgg tagtttccgg tgttgcggcc gcttttttga tgtttctggt tcttatgcaa
    15181 cctgattttg gttccgcttt aatgttaggg gctatttggt tgataatggt aattttagcc
    15241 ggtttcccca aaaaatattt agtggctatt tttgtttctt ctttactgat atttattttg
    15301 gcttggtctt ttttctttaa agattatcag cgtcaacgtc ttttgacttt tttgaatccg
    15361 ggcaacaatt ctttagttga gggttataat atctctcaag caattattgc ggtcggctcc
    15421 ggctcttggc acggcaaggg gatgggccta ggttctcaat cacagttgaa atttttgccg
    15481 gaagcgcaaa atgattttat tttttcagta attgctgagg aatttggttt tttgggcgta
    15541 atggtcgtac ttttgctttt ttctctgttt tttagtcgct gtctggcggc ggtcaaacgt
    15601 ttgcccaacg atttcagtat ttattttgtt ctaggggcgg cgggcttgat ttttattcaa
    15661 atgtttatta atattggtat gaatattggc attatgccgg tggtcggttt gtcgcttcct
    15721 tttattagct acggcgggag ctccatcttc tccttattta tccttaccgg tattattgaa
    15781 aatattatta ttaaatcaaa aattaattat tagccgccgc tggctgacag aaagcgtagc
    15841 taaggcgagt taaaggaaag tatgcaattt aaattatcag ctgaaattag ggaaaaaaat
    15901 gaaaaattag gtcaaggcga tctagccgct gtcttatatg ggaaaggctt agaaagtact
    15961 attttaaaat taaattacaa tactttcact aaagtttttg cagaggccgg cgagtcaaat
    16021 ttaattactt tagagcttaa taaacaagaa tttccagtat tagtcaaggc tgtccaaaaa
    16081 gatgttttaa agggcaaata tttacatatt gatttttata aagttgatat gaaggctaaa
    16141 gtgaaaacgg aaattccttt agaatttaga ggtgaatcgc gagcggttaa agagcttggt
    16201 ggtgtttttt tggttaatat caacgaggtg gccgttgagt gtctgccgag caatttagtc
    16261 gatcatatcg atgtcgacat ctctggctta aatgagtttg gcgatgctat ttatttacaa
    16321 gatcttaaat taccggaagg cattgaattg atgcatgaaa gcaacgaggt tatttgtata
    16381 attgaagaac cgaagagggt ggaggaagag gttccggccg ctgaagtgcc ggcgacggaa
    16441 gcaacgaccg ccaaagaagg ggaagaaaag acggaggata aaaagacgga ggataaaaaa
    16501 tagacttcta tttttacgtc tatggaggat ggaggtagaa aatatttatc taaaaagggc
    16561 agggcgattc ttatctccgc cctttttata ttggtcttct ctggtttagg cttgtattat
    16621 ctagtttttt cccctaattg gcagtggctg tcttttttgg actcttcctc ctccccaacc
    16681 agtacagagg tgccattaag agacgaggcg gttgtggaaa atgatgacgg ttgcgtcgat
    16741 tgtccgatta gttggctaaa tggcttgccg gtggccaatg agaaagaggc ctccgctttt
    16801 ccggtagcgg tggtaattga taatgacgtg ctggctcggc ctcagaatgc tctgagtttg
    16861 gcttctttgg tttatgaagc tccggtggag gggggtatga ctcgctattt agcgattttt
    16921 cccgccaata tcgatgtgag cgcggtcgga ccagtgagga gtgctcggcc atattttgtt
    16981 gcctgggcag aagaattaag agcgttgttt atccattgcg gcggtagtcc ggaagctttg
    17041 gatcttttaa aatcggctaa tttgtacgat ttaaatgaat tttataatgg taattatttt
    17101 tggcgggaga catcagtggt gagaatggca ccgcataatg tcttaactag cggcgataat
    17161 tggcgctcct atttaagcca gcgcggttta gacgaaaaga aagctgattc ttggcttttt
    17221 aagaaggaag tcaaaccgga aaaaacaccg gaaccgaaag atatatcttt gcgttttagc
    17281 gctaattttc aagctttgtg gcgctatctg cccgagtcta acagttatca gcgttatttt
    17341 aacggcgtag aaagtcgcga tttagcgggc ttgattcagg ctaaaaatgt tattatccaa
    17401 aaagtcgaaa gccgaatttt agatgttgcc ggtcgtttgg ccctggattt agaaggagag
    17461 ggcagggcgg agatttgcct ggatggtatt tgtcgctccg gtttttggcg ccaccgtccg
    17521 ggcgagcgta ctcgttatta ttatgatgac ggcgcggaaa tacaattaaa tccaggcata
    17581 acctggattg aagtagctga cagcaatact caccttgaat aatctcttat ttatcaataa
    17641 taataaatct ggtccgacca ctcaggtcgg cttttttttg gatatactgg ggcaagtaat
    17701 atttttttgc ctctttaact aaggcggcgg cttgatgggg attaatttcc atcatgagca
    17761 agagggggcc tgatagtttc aaggtttcta tttgttgaag gagtcggcgg tataattcta
    17821 agccgtcttt tcctccgata agagctaatt tgggttcgta ggcaatactc gtttcccgcg
    17881 ctttctcggc gggagttaaa taagggagat tagcggcaat aaataagggt gagcggcggt
    17941 taatttgtaa atatcggcgc caaggctgga gtaagtcacc gctgcggaaa gaaatttgtg
    18001 actttaagtg ttgtttttta gcgttcttga cggcgatggc caaggcggca ggggaaatat
    18061 cgctagcgcc gaaatagcaa cggccatgac gggaaacttc tttacttttg aggtggtccg
    18121 cgatggcgat aattaaagcc ccggaaccgg tgccaaggtc aagaaagatt tggcggttat
    18181 ggcgcttctt ttggtaatag tcaatcgcag tctccactat aatttcgctt tccggacggg
    18241 gaattaagac ggccggagtg actatcaaag aatgtttgta aaagtttttt tcgcccaaaa
    18301 gataagctaa agggcggcct gtttttaatt gttcgaattt tttggtgaat aacttggttt
    18361 cttgattatt taatttgtat tcggggtggg cgattatcca gctggaatcg cgtccacaaa
    18421 caaaagccag gaggctgaga gcttcccggc taaaagattt ggcggtcgct agatattgcc
    18481 cgatattatt catatctttt attcgcggct ggcttttttt acggttttta ttacctcttc
    18541 caagtcgcca tccatcaggc tgcttaaatt gtgccatgat tggccgatac ggtggtcggt
    18601 catccgatct tgggggaaat tataggttct gattttatca ctccggtcgc cggcgcccac
    18661 ttgatttttg cgggcgctgg aattagcggc tattcgctct tcttcttggc gcgccaagag
    18721 acgggaacga agaacctgaa gcgctttttc tttattttga tgctgtgatt tttggtcctg
    18781 acagctaaca attaaaccgg tgggcagatg aagaatgcgg acagcggagt aggtggtatt
    18841 aacgctttgg ccaccaggac cgctggcaca aaaagtatca acccgaatat cagtcggttt
    18901 aatttcaata tcaacctctt ccgcttccgg caagacaacc accgtggcgg tggaggtgtg
    18961 aatacgccct tgtttttctg tttcggggac gcgttgaacc cggtgagtgc cggcttcgta
    19021 ttttagaaaa ccgaaagccc cctgtccttt aaccgcaaaa attatttctt taaaaccgcc
    19081 gctgccaata gcactagatt caatcatttc tgttttccag ccccggcgtt cgcagaagcg
    19141 gacgtacatg cggaataagt cgcctgcaaa tagggcggct tcatcgccgc cggtaccggc
    19201 tctaatttcg ataatggcat ttttgtggtc gtttggtcct tgcgggtggc attcttcctc
    19261 aatttcttct tttatttttt ccaactgttc tgtaaaagag cttaactcgg ctatggccag
    19321 atcttttaaa tcttcatcag tttcctgatt gatgatatta ttattggcgg cgatttcttt
    19381 aattaaacgc tcctcttcgt taataaggtt aattaaatgc ttcagggcgg catgttcttg
    19441 ggaaacttta atcatagcct ggtgatcttt aatcagttcc gggtctgata atttttgctc
    19501 tatcgccgca aatttttcct taatttcttc gtacatagcc gtttaaacaa gcagctcctg
    19561 attatcttgc ctaaaaatta agctggataa gcaggagctg attgtcttct ttataattcg
    19621 ttattttttc ttggcgttca gagttacctg agcaattttc ttaatgtctt tagtcttaac
    19681 caccacttct ttattggcct tagcctcggc tctagccgct ttcttcactt tcttgccttt
    19741 tttaccagcg ccaatactag ttttagccgc caccctggcg ctaaatttct ctacccggcg
    19801 cgcggtgtca actaattttt gcttaccagt atagaaagga tggcaagcgg aacaaagctc
    19861 tactttgatt tcgggaacag tggatccggt ttcaaagcta ttgccacaga cgcaagtgac
    19921 tacgcaattt ttgtgatatt gaggatggat gtcttttttc atataaatta agggggctgg
    19981 aattgcctcg catattttct taattggcgg taaaggcgca aaaaaagaaa atcaattttt
    20041 tctttagtat ttccacaata tcataacttt aaaaattaat caatacctgt tatactgact
    20101 acagattatt tgcatccttt tatctttttt atgaataaag tagctaatat tgccaaaaat
    20161 acttcttatt tgacgcttgc cttgattatt cagaaattta tttctttttt ttattttatc
    20221 ctgttggcgc gttttttggg actccatgct ttaggccaat attacacggc catttctttt
    20281 actaccgttt ttgcaatttt aatcgattgc ggttttaata atgttttgat tagggaagtg
    20341 gccaaggatc aaagcaaggc ttcagattgg ctccagagcg tgctgagtat caagctatgg
    20401 ctggcgcttg gcacttttat ttttgcgctt atcgttgctt cgtttgctta tgaccaaacc
    20461 ctatttgttt taattattat ttctgcgtgc tcaatggtgc ttgatgcttt tacgagcact
    20521 tttttctcgg ttgctcgcgg ttttcataat ttaaaatatg aaagcattgc ttcggttgtt
    20581 ttccagctta tcgtgttaat ttttggttat ttagctctgt tagcctcttg gccgctgtgg
    20641 cttttattat ccgctttagt ggccgccagc ctgtttaatt ttacttattc ggcttttatt
    20701 attaaacgcc agtttaaact taccatcaga ccacgttggg acgcggctaa ggtgaagcgg
    20761 atatttttaa ttagctggcc cttcgctctt tataacgcct tacaaagatt gtttacttat
    20821 cttgattctt tgcttttagg ttttatcgcc ggttacgccc aagttggttt gtatcaaatt
    20881 gcttttaaac tgatttttgc cctgcagttt ttgccaatgg cctttaccgc ctctttatat
    20941 ccggcaatga gtacttattg gcaagataat cgggaacagt taaggatttc tttcgagcgg
    21001 gcgattaatt atttgattat tatttctttg ccgattatct ttggcgtttt tgccttagcc
    21061 gacaagattg tgcctatttt taaggctggt ccggaggcga tttggcccct gcgtctttct
    21121 attgtcgccc tcttttttat ttttcttaat tttccggtgg gctctttgct taacgcctgc
    21181 gaccgccaac ggcgcaatac tgccaatatg gcgatagcga cggtaattag cgtggcgata
    21241 aatctctttt taattccccg ctggggcgcc ttgggtgcta gttttaccgt cttgttcagc
    21301 aatgctttaa tgttgagttt gggcgcttgg tcaatgcggg gtttaattgt atatcgaccg
    21361 cgccaaaatt ttaaaattct gtggcgttct tttctggcgg ccgctttaat ggctgtaatt
    21421 atctggcagg gtaaagcata tattcatgtc tttttggcta tagccggcgg cggcgtggtc
    21481 tattttttag ctttgatggc tttgggggcc ttaaagaagg cggatataaa cagcgtcatt
    21541 aattcttttc gacgttccta gcttatgaaa atattattag ccacccttga atatcctccc
    21601 caaattggcg gtgttgctag ctactatggc aatcttatcc gtcattggtc gcctgatgat
    21661 gtttggcaag tggtcgataa ctctcaaaat ttacttctag cttctcgggg aatattaccg
    21721 tggcggcgcg ctttattaac tttaagaaga gcaataaata aaaataaacc cgattttttg
    21781 tttctcggtc agatattgcc tttaggaact gtggcttggg ttttacatta ttttaaacct
    21841 ttgccctatg gtattttttt acatggcatg gatttaagtt ttgccctacg ttctcggcgt
    21901 aagcgtcttt taactcgttt aattttacgg aaagcacgta taatcgtctg tgctaattct
    21961 cgcgttcaaa aaatattgct aaatttctat cccgccgcgg cggcaaaagt ggttttaatc
    22021 aatcctggcg ctagtgccgg caaaccggtg gaggagataa aagatagatt aattcaagaa
    22081 tatggattag cggacaaaaa aattattttt tccatggggc gcttggtcaa gcgcaaaggc
    22141 ttcgatcaag taatcgccgc tttagcgaaa gtgccgggag aaaattggcg ctatctcctt
    22201 gctggcgacg gtccggaaag agaggcttta caagctttag ccacggctag tccgcaaagc
    22261 caaaagatta tctttttagg ctctttagga gaagaagaaa aatggtcttg tttagattta
    22321 tgtgatatat ttataatgac ttcccgagat ttagcgggag atttcgaggg cttcggcatt
    22381 gtttatctgg aagctaactt aatggggcga ccagtgattg ccggtaacag cgggggcgtg
    22441 ggcgatgccg tgattaatat ggttaacggc ctggtggtag attctgaaaa tcaagaagaa
    22501 attgctgaag ctatcaatca attgctggcc tcagacgatt tgaagaaaaa acttggcaga
    22561 caaggaaagg tgagggcgga agttgaattt acttggtcta aacaggcgga aactcttcgt
    22621 caatatttat cttccctgaa aaacttatga tttctattat catccccgtt tataatcaag
    22681 ctaaaaaatt gtctttgacc ctggctagca tcgcccggca atcactgagc gattgggaag
    22741 taattgtggt taatgacggt tccagcgata atccagaagc tgtctttgct gatttttgtg
    22801 cgcagattaa aattgataac cgttttattt ttttaagtca ggaaaaccag ggagcggccg
    22861 ccgcgcgtaa ccgtggctat caggaagcta ggggcgagta tctgtttttc tgcgatgcgg
    22921 atgccgattt acagccagac gccttagaaa ttatgcttca ggctttagtg gataatccgg
    22981 cggccagtta cgcctatcct tcattttttt ggggcaagaa gctgtttaaa gtcggacctt
    23041 ttaatgccgc tcgtctgcaa gccggtcctt atattcacac catggctctt attagaagaa
    23101 gtgattttcc cgttaacggt tgggatgaaa gcctccgtaa gtttcaggat tgggacttat
    23161 ggttaacaat gatggaagca gggaagcaag gggtttggat tgataaagtg ttgtttaaaa
    23221 ttactcctgg tggtagtatc agttcttggc taccgtcttt tgcttacaaa ttattacctt
    23281 tcttgccggc ggtaaaaaag tatcggcggg caatggcgat aattaagaaa aaacatgttt
    23341 tggcgtaaat tctcacttcc taaaaaatat ttgtttatag ctttgctgtt attagttttg
    23401 ttggcagctt ttttaatttg gcgttttttc gttcctaaca aatatgacta tttgccagtc
    23461 caagcgggtg attgcatttt tatggtcgag accgcgctta atcccgccca gcaatatcag
    23521 ggattatctc atcgaacctc tttagatgcg gataaaggta tgttattttt attttctcca
    23581 gccgccgaca aaacttttgt aatgagaaaa atgaattttc ccttagatct tatctttatt
    23641 cgcaatcatc gcatagttaa tttatatcat gaggcggcgc cggaagggaa aagtccggaa
    23701 acctcttatc atagtggcgt gcctgttgat gctgttttgg aaattaaggc tggacgcagc
    23761 cgcgattgcc acttgggtgt aggctccgaa ataacttggt aggctatgaa tttatttaaa
    23821 gtaaaatccc catatttttt tattttcagt ttgataattc tggttgaact tttgtctttt
    23881 actgcttatt ttttacctca actgcaaata tatttgttga taaccgcttt tttggcggtt
    23941 tttttcttaa gtttatcttc tttagaatcg ggtctcttgg tagctttagc ggaattggtt
    24001 attggttcaa aaggacatct tttttccgcc tctatcttcg gcgtctccgt atcgttgcga
    24061 ttggtaattt ggttggcatt aatgttggcc tcctgtattt ttatcttccg tcgcggcttc
    24121 cccacagtat ggcggaccaa actgtttaat ttctcctatt ggccttgggt gtcagtttta
    24181 gccgctttta ttatcttagc ttttgttcat ggttatctcc gcggcaatgg cgctagcgat
    24241 atttttaccg atggcaacgc ttggttttat cttggacttt tattgccgac gatattagcc
    24301 tattcccaaa ttgacgacaa acaagagcaa cgcttgcgag ccgtcttttt tacggctatt
    24361 ttttggttga gtctgaaaac cttgatgttg ctctttatct tttcacacaa catcagcatc
    24421 atgcccgatg tttacctttg gattagacgg agcggcgtcg gtgaaattac ggctatgggc
    24481 ggtggctggt atcgtatttt tatccagtcg caaatatata tacccattgc cttttttctt
    24541 ttgctttggc cgtctttaga cttgaaaacc aaaaaaaatc cctatctatt gataattagt
    24601 ctcgctgttt tgttgtcagt aattattatt agcatgtcgc gcagtttttg gttggccttc
    24661 gccttcacgg caggagtgat ggcggtgata agttttagga agaaaattag tttatatcta
    24721 aaggctttag tctatactgg tgctgctttg ggcttgtctt tgttgttgat tttagtggta
    24781 gtgaaatttc cgtatccgaa tcctcagact ggcttatcag cggcggcatt aagcgccaga
    24841 ctagatttca gtagcgggga agcggccata tcttctcgct ggtctctttt acctaattta
    24901 tggcaaagta ttaacgattc gttgttttgg ggcaagggct tcggcgccag tgtttcctat
    24961 ttttctcaag acccgcgcgt cctctctcta aatccaagcg gctggtatac gacttatgcc
    25021 tttgaatggg cctatcttga tacttggctg aaattgggat tattcggttt aatcgcttat
    25081 tttgcctggc taatcgtttt gattgttgat ttgcttaaaa aaatgcggga gcatggcaag
    25141 atgctgccag cggcaatttt ggccgggttg atatttttaa tggtagtcaa tatttttacc
    25201 ccttatctca atcatccttt aggtttcggc tttctgatat tttcctcttg ttttatcaaa
    25261 aaaaatcctt tataattcat actaattgtc gcgtccggcc ttattgccca aggcgcctta
    25321 atctttaatt tctaatttat gtctgaacaa attattactc aagaaggtta tgataaatta
    25381 caggatgagc tcaattatct aagcttaatc aagcgtcgag aaattgccga aagaatcgaa
    25441 cgcgccaaag atttgggcga tttgagcgaa aacgcggaat atagcgaagc taaagacgcg
    25501 caagctttaa atgagggcag aattttagaa ataactaata ttctcaaaaa tgtcacggtg
    25561 gttaataatg agggcggaca cgaagatgta accatggggt catgtgttac cgttaaatgc
    25621 gacggcaaag aaaaaaagta tacgattgtt agcttcaatg aagccgatcc aattaatggc
    25681 aaaatttcta atgagtctcc tctgggggtg gctttcttgg gtaaaaagaa gggggctatc
    25741 gttcaggtgg aaacaccgcg aggaatcgtc gaatataaaa ttgtaaaaat agattaaaat
    25801 atatgtccgc agaaaaaaat ctggcgccag gcagtgaaag agaggagcgt ctaaaaaagc
    25861 ttgaggcttt aaaagctgcc ggtattaatc cttatccagc aaagagccaa cgtaattata
    25921 gtattgccca gattttttct gatatggaaa aattagaagc caatcaaaca aaagttataa
    25981 ttgctggccg tttgcgcgaa gtgcgtggcc acggcaattt gacttttgct catcttgaag
    26041 atgcttccgg tcgcatccaa ttagcttttt cccaaaagga attaggccat gaaagctata
    26101 aaaggttggt caaattaatt gatagcggcg attttcttga gactgccggc accatttttg
    26161 tcacccatag tggtgaaaaa agcttaatgg tatccgattg gaaggtgtta agcaaggcga
    26221 ttgcgcctat tcctaatgct tggtatggct taaaagacga agatgagcgc tatcgccacc
    26281 gttatttaga tattttatta aaccccgaaa tcaaagatat tttctataaa aaagccaaat
    26341 tttgggaagt tactcgtgat tttatgaagc gccacggttt tttagaggtg gagactccaa
    26401 ctttagaaac taccaccggc ggcgctgagg ccaatccttt ccgcacccat catgatgatt
    26461 ttgatttaga tgttttttta cgcatttccg taggcgagtt gtggcaaaag agattaatgg
    26521 cagccggctt tgaaaaagtt ttcgaaattg gtcgcgtcta tcgcaatgaa ggttctagcc
    26581 ctgatcatct gcaggaattt accaatatgg aattctattg ggcctacgct aattacgaga
    26641 tgggaatgaa attaacccag gaattatatc aagaaatcgc tttaaaggtt tttggtagaa
    26701 cgaaattcag cactaagggc atggaatatg atttggccgg tgattggcct aaactggatt
    26761 atcgggaaac tgttttagaa aagacaggga ttgacatcct taaagccagc gaagaagaaa
    26821 taaaatctaa attagacgaa ttacaagtaa aatatgaggg caataatcgc gaacgtttaa
    26881 tggatactct ttggaaatat tgtcgtaaaa gcatggctgg tccggtattt ttagtcaatc
    26941 atcctaaatt agtctcgcct ttatctaaag ccaagcccga aaaccctgaa ttaacggaac
    27001 gttttcaaat tattattgcc ggcagtgaag tcggcaacgg cttttctgaa ttaaacgatc
    27061 cgattgatca gcgccagcgt tttgagcttc agcagaaact gatagacagg ggcgacaagg
    27121 aagcgatgat gcctgattgg gaatttgtgg aaatgcttga gcacggtatg cccccgactt
    27181 gcggctttgg ttttggcgag cgtttgtttg cctttatggt ggacaagccg gtgagagaaa
    27241 cgacgctttt tcctttaatg aggccgaaaa gggaggagat ggaaaagaaa gattcaccct
    27301 tgaaaaaaaa taagtaattg atataatagc cgtatcattg aaatggcttt aagtaattag
    27361 gccattttta ttcggggatc gtctaatggt aggacaacag gttctggtcc tgtttatcgg
    27421 ggttcgaatc cctgtccccg agccaacacg attttggtgt agtagtccga gatacttcgt
    27481 ctaatatcag ccaaaaaaca aagaaactca gtttacgctg agtttttttt gttggagtca
    27541 agtgagtgaa ataatctgta aataaagaaa attattaatt cgaatccaag gtctgttttt
    27601 attttgtgat atagtttata atataattac aattgaataa tcgttttata aagagcgcag
    27661 cgagggaact agcccgatga cctgccagca accctgaagt aatccattca gaaggtgcta
    27721 aatctagccc gattagggaa agataaaggt cgatttttta ttttcataaa taaaaactct
    27781 ttcctttttc gggaagagct ttttattatt aattaataaa tttatggaaa tcataaagca
    27841 gagcattagt ttatgtgaca agtgtaataa ctggaaagga acagtagaag aaagatataa
    27901 cgatttagtt ctagttcatt gtgtttgcac cttatcaagt agccagaaaa atagcacaag
    27961 aagcctaagc ataatttgtc caaatagaga taaattatgg tggacgccaa tttcggaaca
    28021 cattgcagaa gatggaaaat ggtatcatgc tgcgcacttc gccgggccaa cacttaataa
    28081 aaattaatta aataagatat atgaaaaatg aaaatactat aaaaataggg aaaacattta
    28141 ctacagatgg gcatagagag gaaaaggaga tatattgcct agaagatggt gaggagcggt
    28201 tttataatat tctaggtacg ggctatggaa taaaccttgt tcattgtttt tccatggggg
    28261 caaaaaataa agaaaatttt tgggaaaggg tctttgctta taaaatcatt cccaacagag
    28321 aacccgccaa tttaatgaaa agagtaaccg gaaaagacta tatatcagta gaattctatg
    28381 aattaagcta aatttagcga ttttgatgtt acaattgcag caattagggt tacctttcgg
    28441 gttccgaacc catataatat g
//
LOCUS       DAOQSA010000012        28298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_1019_length_28298_cov_9.274333, whole genome shotgun sequence.
ACCESSION   DAOQSA010000012 DAOQSA010000000
VERSION     DAOQSA010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 28298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28298
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1019_length_28298_cov_9.274333"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="PKK65_02010"
     CDS             complement(<1..734)
                     /locus_tag="PKK65_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81488.1"
                     /translation="MAKFGFLIKKHDKLGRFIYLLLVLMKLKTISRLVLRAPGAAWLF
                     LFFSIFSTLVLISPAQAITGDYNKPVTGTLAASEWNNLVNDFVAKSGARLDGNFSINT
                     ATSSGYALDISGILRASHFSGSLSAADVSTGVFGQNTGGGNFNFLGNVGIGIGTASPA
                     YTLDVAGIINANALYVNGAPYIGSQWISNGTSLYYNAGNVGIGISNPSAKFEVSGGNI
                     FINDALITAATPKAAITKEYLDSALA"
     gene            complement(879..1253)
                     /locus_tag="PKK65_02015"
     CDS             complement(879..1253)
                     /locus_tag="PKK65_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HNU81489.1"
                     /translation="MIIKLYKHQRGSAIMLTIFILSAMMLIIFSSATIVVSGLKMGGV
                     QSQSTRAYFAAEAGAERLLYDFRKTNAFDDLSQLGQDNIFGTTTLSTGSSYVVNYDHF
                     NPIIFTSIGIYENTRRSVELDF"
     gene            complement(1255..1821)
                     /locus_tag="PKK65_02020"
     CDS             complement(1255..1821)
                     /locus_tag="PKK65_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81490.1"
                     /translation="MPALKKRYKNHRGISLIEVIISTSLFVVILLSMTDIFRLILNSQ
                     REAIATQNVQENLKYFFEVISKEIRMAKRAAGGCAHLPANRRFATSTNAYGDVLYLKN
                     YHDQCVAYYLSDDNGVIRFKVERGFDSAFISPAQINIDDLHFIVHEDDDAQAYVSINL
                     SARSLGREADVSEMRVQTTIASRYYRSD"
     gene            complement(1823..2398)
                     /locus_tag="PKK65_02025"
     CDS             complement(1823..2398)
                     /locus_tag="PKK65_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNU81491.1"
                     /translation="MIGNKDRKVSLKTGFTLIELVVSLSIISILTALFLVNYHSTNQR
                     TDLTMSAQTLVTNIRFAQANALGLVKYDGEIPPGGWGVFVSSGSGSNHSYFIFADEND
                     NRKYDNGEAIPALGGNVISLSPNISIDSLSLGNVEVSQANVTFLPPDPITRLESGSAT
                     STFLDIRLRESLNNTTKTVRVNFLGLVEVID"
     gene            complement(2385..2900)
                     /locus_tag="PKK65_02030"
     CDS             complement(2385..2900)
                     /locus_tag="PKK65_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81492.1"
                     /translation="MFKRLRTISRCHPAFSIIEIMAVLLIVSLAMIGVANLAVQSIQA
                     QTINRGSIIAYQLAQEGIEIVRQIRDTNWLQGKNWKDGINSGDYCVDYLSPTLRVASS
                     LNDCPLYFNNDNWYYSPVTVAANSRRSNFKRLISINAATSSASVKATITWNDRDKVFT
                     YEVETELYDWK"
     gene            complement(2910..4265)
                     /locus_tag="PKK65_02035"
     CDS             complement(2910..4265)
                     /locus_tag="PKK65_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81493.1"
                     /translation="MRLRHFLFLLLPFAALLLPSSLEVAHASINENIRGLASSSREYI
                     SFNCLDDDFAGRFPFTFPFRFNVPPCSFSQHGVNLNSDNTFSGLAWNPVLGFIDFGST
                     STPAAPNYDFNVNCQQPCNASNNCIACYNDENERVFGWAYVIGTDEWIELNSAIMPET
                     TMTNYLTPQPGIFSGYASSSFGAIMFNCANNGSCGTFDHKVYLWKLDLKKMSAPNWSF
                     SDACSQGARRVTFKWWRRGGIQTAYRVVVNTTNSTSSPVYDSGQIAGFASQLVCPGPN
                     CSFQPAYGTSYYWWLQLWDENNQPTELFPFDTNTFGVLTDNIAANSVNNPVDPHLTFT
                     NYKHEFPTPYFSWSPTEIIVGSSTDFVSNSRYYTVASPNSNPQLCVDGVCQFWWQVSD
                     GAAYISQATQSTTTIVFINKNPKSVSLQITDPDLYTCSTSSPVLTINFQLPLWREVKA
                     E"
     gene            complement(4268..4723)
                     /gene="ybeY"
                     /locus_tag="PKK65_02040"
     CDS             complement(4268..4723)
                     /gene="ybeY"
                     /locus_tag="PKK65_02040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNU81494.1"
                     /translation="MFSIEIKNLSKRPVGLKVLRRAAEFTAQRLHLDGELSLVLAGDR
                     RLQRLNQAFLGKDKPTDVLSFRAPVISPGALGEIFINLSDCRRPQKYLAVFGAQKSFT
                     YILLFLMIHGLLHLAGFDDATEKERLAMISKGERIMKELVKNAIIKENL"
     gene            complement(4725..5711)
                     /locus_tag="PKK65_02045"
     CDS             complement(4725..5711)
                     /locus_tag="PKK65_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HNU81495.1"
                     /translation="MILTKEDNLADLTWEASLQSLPDGGELLQSGIWRQIALAEKREV
                     KRFSWLQEKGMVAVLAQVLTTNRFGLKSWYLPRGPVWLLSEGAEIAWPLVLADLIQEA
                     KRQGVMSIRFEPENWPLPVVSFGQKITSIQPEKSLFLDLSYSEEELLSQMHPKTRYNI
                     RLAEKKGVEIVSGQSEDLVDFWQLLQTTTARDGFRGHSLAHYRHLLEKGGGAIELYFA
                     RLEGKILAAGLFSFYGGRAVYLHGASANNDRQYMAPYLLQWRMIQRAKAKHCRYYDFY
                     GIDEKRWPGVTRFKRGFGGEERVYPGSFLLVISKHKYFIYSLLSKIVKKLRP"
     gene            complement(5794..6210)
                     /locus_tag="PKK65_02050"
     CDS             complement(5794..6210)
                     /locus_tag="PKK65_02050"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNU81496.1"
                     /translation="MNNKKGFTLIELLVVIAIIGLLSTMSVLALNSARARARDAKRIS
                     DIKQIQTALEMYYNDNNNYPNILDQLTTTTPPLIKVIPTAPLPVDGTCDDTENTYTYT
                     YSGSGSTASYALTYCLGSATGNVPAGSHTATPAGIQ"
     gene            complement(6369..7577)
                     /locus_tag="PKK65_02055"
     CDS             complement(6369..7577)
                     /locus_tag="PKK65_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNU81497.1"
                     /translation="MPIFKYKAKNNITGKTKSELTVGINEADVVKRLSRQNFSVLAIA
                     DKTDSLEAKINLFLNSIKVKDLVIFSRQFSVMIAANVPVVESLLILIDQTNNFSLKKM
                     IADIAFEVDSGALLSDAFAKRPKIFSEFFVNIIKSGETSGKLDEVLTYLADETEKSYD
                     MASKIKGAMIYPAFILVGLLAVAVILMVYVIPNLTSMLTETGMQLPLATRIIIAISNF
                     LQNYLILLILTLVGLIIAVRYYLRTEVGRYQLDTFKLKMPIFGRLFKYIYLVRFSRIL
                     STLLKGGVTITRALEITANVVGNVVYRDLILATLESINDGNPLSTVMEASNDVPKMVP
                     QMLSVGERTGRIDSVLDKITDFYGRESSAMLANLSTLMEPIIMVIMGVGVGIMVAAIL
                     MPMYNMANQF"
     gene            complement(7598..9361)
                     /locus_tag="PKK65_02060"
     CDS             complement(7598..9361)
                     /locus_tag="PKK65_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNU81498.1"
                     /translation="MLIDQQEQLLKILQEKKLLVADQVADIKLKVAAAKTELPDFLLN
                     NKLVNEEQLTEAKAVLYNLPYFAYTDEVIPKEVLNFLPEEIARTYNIVCLAKENKTLK
                     IGLVEPNLKAMEAVNFLALDEKLNVQYYLLSASSWQKIFKQYQKIEEEISSALEIKAK
                     EDSDDVVAIKSEDAAMVDEEDINSAPVSRIVFVIIRHAVEARASDIHIEPYDKESRVR
                     YRIDGILHTSLTLPKSIHNAIIARIKVMSKLKLDETRVPQDGRVRLLVNGRAVDFRIS
                     TLPLANHEKVVMRILDTAKGAPALSDLGFNSNSLRAINAAIKKTSGIILVTGPTGSGK
                     TTTLYSLLNILNQEGVNISTLEDPIEYEIKGVNQSQVRPKVGFTFANGLRSLLRQDPN
                     IIMVGEIRDEETAELSIHASLTGHLVISTLHTNDALGAVFRLLDMKIERFLLASTLKT
                     VVAQRLARRLCDKCRQVVTLPESELAAMSAALKDVPAHILKTELPDLQDLNNLSNYTF
                     YRSVGCSHCENTGFTGRVAISEVIDINDQLKEMINRGDKNLDIDAVKASQDFISINQD
                     GIIKVLKGITTMEEILRVIES"
     gene            complement(9379..9741)
                     /locus_tag="PKK65_02065"
     CDS             complement(9379..9741)
                     /locus_tag="PKK65_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81499.1"
                     /translation="MSKSRSSSIYILTGALIIVGLLIFAFRERLLSYLSARVFGAAVD
                     SSETVLNVSEAGNLDIKILNNQTFNNLSNHVLYFDFDKVGKPVLNRAANPNLQAPLWP
                     AVYLGNSHLFPVKEKKEN"
     gene            complement(9748..>10322)
                     /locus_tag="PKK65_02070"
     CDS             complement(9748..>10322)
                     /locus_tag="PKK65_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81500.1"
                     /translation="TNFNILVVLAVIIFLAAAYFLIIKPKFELTLVAIKDDISQQEQL
                     YQRQRQKLIDLQAAAAMYHKISDSDIYKIKEVLPDNYAKEKLFGELEDIITQQGLLIT
                     GINLEKIDEVDSEAEPLAAKDEQRLAIPNSEHVGVIMAIINLASIDYASLKNLLSLFE
                     SNLRLIDIDAINFDQEEKTAEITLYTYYFK"
     assembly_gap    10323..10422
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10503..11159)
                     /locus_tag="PKK65_02075"
     CDS             complement(10503..11159)
                     /locus_tag="PKK65_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81501.1"
                     /translation="MFNFSIKPKKKSKTVVSSSQNPKVLEVNLIKSEMPVDFEVGKNI
                     GTLLFALVIAALFVAEVYLGLNWWSDYENVRMLAAETKFNQVSKEIKALKTESDQILA
                     FKQRADLANTLLENHIYWTNFFNWLEKNTLSSVSYLGFQGDKSGVYSLDASAKTFRDI
                     SWQTRAMLESPAVISVHVNKGESERPTDSGGAEAEVENIKFNLDLVVDPNIFKNSAAN
                     "
     gene            complement(11170..12219)
                     /gene="pilM"
                     /locus_tag="PKK65_02080"
     CDS             complement(11170..12219)
                     /gene="pilM"
                     /locus_tag="PKK65_02080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HNU81502.1"
                     /translation="MGLFSSAGDNLFLGIDIGDSSLKMVELKKKNKKIFLSNYAFSEN
                     VSGVNFTKIDDINYLAQAILKVKAKAGIKAKRVTASLPTFSVFSSIINLPPTDKKNMD
                     AAVAEEAKKVIPLPVEEMILDWKLVPTGKDGNKEGMRVFLTGSPKKLVRRYIEIFRLA
                     KLELASLETETFSLVRALMGNDPSTVMIVEIGANSTDLSVVHESIPVLNRSLEICGST
                     VTAVLAEKLGLSFNQAEQFKFDLGASLSDVSQEELPQLIIKTLAPVIHEIEYMREFYQ
                     NNAGGQKIEKIILSGGGALLMNLADYLSRRLNLQVIIGNPFSRIIYPSEIEPIIAEVG
                     PKLAVAAGLALREIS"
     gene            12390..13661
                     /locus_tag="PKK65_02085"
     CDS             12390..13661
                     /locus_tag="PKK65_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81503.1"
                     /translation="MGFFSKYKKIWLLLGFIAFVGLMIYLIWITFFRNPLTPPAVTEI
                     TGNIGGLNPADEGSGNYSSTSGDGRLNVSEDNPSNRQNNGQISTPAPTGTEISDRALG
                     GITKVTALTSDPVLSPTMTSNDELRYYNQNDGKFYKLDSNGELVTISDKIFHQVSKVT
                     WSTDGNKAILEYPDGSKITYDFQTQKQVTLPKHWEDFSFSPSGKQISAKSIGNDVENR
                     YLITANADGSKAVSLSAIGENADYVYPVWSPNNQAAAMYTRGLDFNRQELFFVGLNGE
                     NFKSTIINGRGFEPKWSDEGDKLLYSVYSSDNNMNPSLWIVNAQGDNIGRNRTNLGLS
                     TWASKCTFASNEEIYCAVPENLPQGAGLFPELADKTTDNLYKIDLNTGAKKLIAVPST
                     AGNISNIMVDSNQKTLYFTDKNTGKIYEIKL"
     gene            13713..14420
                     /locus_tag="PKK65_02090"
     CDS             13713..14420
                     /locus_tag="PKK65_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNU81504.1"
                     /translation="MFKKVINLLLKIENFILPETCLNCRRAGQALCPACRVQIKKIPE
                     RCPTCGRRQNLGLFCQKCRQTHRTISFDGIFAYTSEDDQIMIKAIKALKYQGVKSLGR
                     ILGKMLGRRLVISWKQAPASIKKRNTIIIPMPLHPRRQRARGFNHSQLIAAGIAQVSG
                     WLINENLKRKAWHKPNAVSLDNQKTFVYKGKLLTGNNIILVDDAIATGATAQAAAQAL
                     KTAGADTVIVATLTQSF"
     gene            14667..16403
                     /gene="thrS"
                     /locus_tag="PKK65_02095"
     CDS             14667..16403
                     /gene="thrS"
                     /locus_tag="PKK65_02095"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNU81505.1"
                     /translation="MSTLETKRHSLAHLMAAAVKEMWPQAKLAIGPAIDNGFYYDIDF
                     AGASINDTQLKEIEKKMTHLVKQNLEFTRTEKNIEEALAWARAEKDPYKEEIISDLKE
                     KGETTVSFYTVGQFTDLCRGPHVENTKEIKPHSFKLDKLAGAYWRGDEKNKMLTRLYG
                     LAFDTKEELDEYLKMMAEAEKRDHRQLGKDLDLFMNHELAPGMPFFLPKGMILLQELL
                     KFVRSYSYGDGYYEVRTPQILNAELWKISGHWEHYQEDMFCLHHAEDDIDLGIKPMNC
                     PAHMLIFKRDLHSYKELPLRLAETTTLYRNEKSGTLHGLTRVRSLSQDDSHIFARPDQ
                     ILDEISTLLDKIKMIYRVFDLEIDEIHLSTRPAHFLGEKSVWDEAEANLKQALEKAKL
                     AYHINEGDGAFYGPKIDVKVKDAIGRQWQLATIQLDFQLPQRFALKYTEADGSAKTPV
                     VIHRALLGSMERFLGVIIEHYAGAFPLWLSPVQVKLISVAKTHLPACQKLANELKEAG
                     IRVEIDENNETVGNKIRKAIKEKVPYMLVIGDKEAAAEKLVVRDRGAKETREISREDF
                     LKELTDKIKNYT"
     gene            16492..16866
                     /locus_tag="PKK65_02100"
     CDS             16492..16866
                     /locus_tag="PKK65_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81506.1"
                     /translation="MNKKKSALSAIIILLVLVVVVSIFISKRQTGTEQKTSSDNGPIL
                     FYSLSCPHCQNVEKYIQENNIKNKYSFSQLEVSANKQNREILVAKAKICGLDTQSLGV
                     PFLWTGEKCLLGDTDIIDFFSK"
     gene            16871..17383
                     /locus_tag="PKK65_02105"
     CDS             16871..17383
                     /locus_tag="PKK65_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81507.1"
                     /translation="MKKKYLFALTLAFVLKALPAKAVCPVCLVAAGAGLGLSEYLGID
                     DSIAGLWIGGLLIGLSAWTITYFNKKNWLKNYSAWRDPLIIIVYYLMVIWPLYSQGFI
                     GNPFNRLYGVDKLALGITVGSIFFALAAWSYELMKKKNGGHAHFPFEKVVLPISTLVI
                     LSVIFYFLSR"
     gene            17388..17927
                     /locus_tag="PKK65_02110"
     CDS             17388..17927
                     /locus_tag="PKK65_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81508.1"
                     /translation="MDLSKNPIKSLQEFINKADDMKKQEKMDLSSDQDLTIAIMNLVS
                     IEEHFFFTGAKTGKNEYYDLINEVREIRKELLKKIIKEYEGEIWCISKHLLAASYRLM
                     EVGTKQLSRGQKEEAYSLFDKAYQLYSLFWGLNLKLIGTGDIKKIDEKAIDKHDEEKK
                     GFTDKLGELVKKVINCCLE"
     gene            complement(17997..18308)
                     /locus_tag="PKK65_02115"
     CDS             complement(17997..18308)
                     /locus_tag="PKK65_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81509.1"
                     /translation="MEKKNGVGNIVVGESNFATILPGHIFKVPEVRKLFHPHAVVMYF
                     SETLEQVIPGLWLIEIILVDNPDTRLVIDLAKLNLSERQREELIKYKGVPKDFFRLKN
                     N"
     gene            complement(18428..19270)
                     /locus_tag="PKK65_02120"
     CDS             complement(18428..19270)
                     /locus_tag="PKK65_02120"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HNU81510.1"
                     /translation="MKKIFFFLIIIAAAIFFLMDKGQPGSENNESVPVTAEVLPTASI
                     GLPEREVASLDEKEKRENVLEADIKIKAAAPLASSHALINGNENIILSVPFLSQAPLA
                     NWEDARQQDGCEEAGALMAMAWARGEIDTSPKASEKAIIALADWQQEKYGEHRDVYID
                     DVAVRIFQDYFHYDKVKTQVIIDKNDLISALKEGKLILAPVNGQALHNPNFKAPGPER
                     HLVVIIGYDNKTDEFITNDPGTRRGASYRYSSEILFPAIRAYPSGYHQPLVGVEKKVL
                     LVEK"
     gene            complement(19276..20412)
                     /locus_tag="PKK65_02125"
     CDS             complement(19276..20412)
                     /locus_tag="PKK65_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015550868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HNU81511.1"
                     /translation="MSDLNAESLELHRFYGGKIATALSIPLNNQHDLSLAYTPGVAAV
                     CQAVAADKEAARTHTIKGRTIAVVSDGSAVLGLGNIGSVAALPVMEGKCALFKKFAGV
                     DAFPICLDTQDTEEIIKTVKYLAPVFGGINLEDIAAPRCFTIERRLQMELDIPVMHDD
                     QHGTAAVVLAALINALKLRGSDRSDIKVVISGAGAAGNAIARLLFGYGVKNIIACDRQ
                     GAIYVGRPDLDKEKADLATFTNLGKQKGALAEVLIGADVFIGVSSAGLLTREMVRTMA
                     PKSLVFALANPVPEIMPDEAKAAGAFIVASGRSDFPNQINNVLIFPGVFRGALDNKVR
                     QISDERLVKAAENLAALVSVPRVDRILPEPFTPGIAAAVAAAIK"
     gene            complement(20828..21658)
                     /locus_tag="PKK65_02130"
     CDS             complement(20828..21658)
                     /locus_tag="PKK65_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81512.1"
                     /translation="MKKIITFAVLTVIGVAVVGLASARAEDSAVSVTAIATSTAAGSL
                     EKIPSPDQIKYFKVIKKENGSLYGIRLSTAVKNQATEQISSHNQEKTNTNKAQLEKIL
                     APQFINLYEKIQKIGNSLWGVKKETKSVDNAGPKATDQAIKPMPKPRLVTAEMIACVS
                     AAIDKKDKALQNQIDLSAESLKAIISTRGECQKKAIQTTAGQLENLNICIKDFQAKYQ
                     EIIKQSKDEQQIIWKAYQSDLKACSASAADNALDSAVESSELMIEDGGGAVIEGVLMQ
                     "
     gene            complement(21741..22055)
                     /locus_tag="PKK65_02135"
     CDS             complement(21741..22055)
                     /locus_tag="PKK65_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81513.1"
                     /translation="MNSLFPNILDLLLKTLKDFINSDLLAIILVVLFLLFLIIVWLLI
                     RELLTWYWKINKIVSILEKIEVGVDFLAANADQNNKDQEKDKAVFIKKPIEEVKSQEP
                     NK"
     gene            complement(22088..22315)
                     /locus_tag="PKK65_02140"
     CDS             complement(22088..22315)
                     /locus_tag="PKK65_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81514.1"
                     /translation="MSNTLDIDFCPRALERTYEITIPDIMNSDQGQSLNDKTPTEVYF
                     GQNKKTVFFQQFTNLHINSNIVPTKSTFSWF"
     gene            complement(22482..23195)
                     /locus_tag="PKK65_02145"
     CDS             complement(22482..23195)
                     /locus_tag="PKK65_02145"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNU81515.1"
                     /translation="MLNKEKHQLIMGQVLKDIYTDVSISPLLGFKGDTCTYFFYNLPR
                     FSVDLDFDLLEVTEENQKMVFEKIVAILSKYGTIKDQYIKRFTVFALLSYGDEDHNVK
                     VEINVRKLAENMKDLYEMKEYLGISMMVAKKDYLFAGKLAALTLRTETAMRDIFDIQY
                     FAKNHWDINAEVIKNFTGKTVKEYLPDCIAFIEKIKDSQMLQGLGELIDNEKQKDWVR
                     NHLKADAIFMLKNYQSVLK"
     gene            complement(23200..23769)
                     /locus_tag="PKK65_02150"
     CDS             complement(23200..23769)
                     /locus_tag="PKK65_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81516.1"
                     /translation="MDSLIAKLYQSNKTILTSKDLALVWGENDQIKLKDKISYYVRRG
                     VLIRLTRGVFAKDKNFNAKELATSLYTPSYISFETVLREAGVIFQHYNTIFVATKWPK
                     IMTVDKYSITFRKLKDTVLFNSAGIESKDNYSIATPERAFLDMIYLSPNYYFDNLKGI
                     NWEKCDELVKIYNNKSLIKRLNKYRKNSQ"
     gene            24064..25800
                     /locus_tag="PKK65_02155"
     CDS             24064..25800
                     /locus_tag="PKK65_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNU81517.1"
                     /translation="MKYLAKYIKPYLIGLLALLFFVWGQSYVNLILPDYTAKIIDQGI
                     MLKNINIIWSEGTKMLLLTILGGLCTILIGFIAAKVSAGFAKRLRKAVFDKIERFSLN
                     EFNIFSSSSLITRATNDVQQIQNVLSMMLRMSLLAPFMGIGAIIKVYQLAPSLSWIIL
                     TAICVLALMITILFLIAIPKFTIIQKMVDKLGLQIREMLTGIRVIRAYNNDQKQQLKF
                     DKTNTELMNLNILINRLISMMQPAMTLIMGLSAVAVIWAGAYLVASGHLQIGSILALT
                     QYVSQAIMAFLMISIIFIIIPRATVSVRRINEILSIIPEINDPAEPKHLTEKIHGLLE
                     FKKVDFSYKGSEQAVLSDISFIAYPGQTTAIIGGTGSGKTTLLNLIPRLYDVTGGAIT
                     IDGIDIRDLPQHELREYIGYVPQKASLFSGTIKSNVAYGRPSASLTEITEAINTAQAA
                     EFINDLADGLDSAIAQAGNNVSGGQKQRLAIARALVKKPPIFLFDDSFSALDFKTDAA
                     LRQALQNNLKTSTIIIVAQRISTIMQADNIIVLDNGKIVGQGKHQDLLTSSAVYREIA
                     ESQLSATELKKK"
     gene            25802..27619
                     /locus_tag="PKK65_02160"
     CDS             25802..27619
                     /locus_tag="PKK65_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNU81518.1"
                     /translation="MAKIVNGPFRGGSPRTLMKGKTEKAKNFKKTFKIFIKYLKPYRL
                     KLILVLLFALAATAFTIASPKILGQMTDVIVRGLLNHNKIDFVQITKIGLGLICLYAA
                     SALFSYLQSWIMSKISQKITYSFRHNIAAKISRLPLSYFDHNEKGDIISRVTNDVETI
                     SQNLNQGLVQAITAAATVIGILIMMLIISWQMTLIALGILPLSFIFIKLIVKHSQHHY
                     DTQQSSLGKINGHIEEMFSNHAIVKAFNGEERSINHFDDINADLYNSGWKSQFLSGLM
                     MPLMHFISNLGYVAVAVVSGWLALMGKVSIGGIQAFIQYMNQFTQPITQTANIASVFQ
                     STAAAAERIFEFLEEEEEKPETNNTPKIKNVRGRVEFNKINFGYFPDRPIIKDFTAQI
                     KPKSRVAIVGPTGAGKTTIVNLLMRFYDIDSGEILIDGTNIEKMKRSDVRSLFGMVLQ
                     DTWLFNDTIAENIAFGKSGATREEIIEAAQEAQIDHLIRTMPNGYDTIISDSIDSISV
                     GEKQLLTIARAILADAPMLILDEATSSVDTRTEILIQTAMEKLTKNRTSFVIAHRLST
                     IKNADLILVMQNGNIVEQGQHQELLDKKGFYYSLYNSQF"
     gene            complement(27757..28149)
                     /locus_tag="PKK65_02165"
     CDS             complement(27757..28149)
                     /locus_tag="PKK65_02165"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNU81519.1"
                     /translation="MSKIIIASGPVIVENNRVLLNKHGDDEFWKFCGGRVEEGETNLK
                     EAAIREVKEEMGLDIEILNNNPYFFYTEKEKEGENLSVILVHFLAKRIGEVVPGKDIK
                     EWRWIDIQDLDKEDLAPNIKPVLKYFGF"
BASE COUNT         8718 a         6211 c         5497 g         7772 t
ORIGIN      
        1 ctggctaaag cagaatccag gtattctttg gtaatcgccg ctttcggcgt ggcggcagta
       61 atcaaggcgt cattgataaa aatattaccg cctgatacct caaatttagc ggaaggatta
      121 ctgataccga tgccaacgtt accggcatta taataaaggc tggtgccgtt actaatccac
      181 tggctgccga tatagggagc gccgttaaca taaagggcgt tggcattaat aataccagcc
      241 acgtctaacg tgtaggcagg agaggcggtg ccgatgccga tgccgacatt gcctagaaaa
      301 ttaaaattac cgccgccggt attctgaccg aaaacgccag tagagacatc ggcggcgcta
      361 agacttccgg aaaaatgact ggcgcgcaaa ataccggaaa tatctaaagc atagcccgaa
      421 gaagtggcgg tgttaatact gaaattacca tctaagcggg cgccgctctt cgccacaaaa
      481 tcattgacca gattattcca ctcgctcgcg gctaaagttc ccgtcaccgg cttattataa
      541 tcaccggtaa tcgcttgggc gggagaaatt aaaaccaaag tggaaaaaat cgagaaaaac
      601 aaaaataacc aagccgctcc tggcgcccgg agtactaacc tgcttattgt tttcagcttc
      661 atcagcacaa gcagtagata aataaaacgg cctaatttgt cgtgcttttt tatcaagaag
      721 ccaaacttgg ccaagcgata aaataagacc gaatttatga taaaattata gcatttttta
      781 aaaagcaagg aaagtttgaa acccttaaaa aaaacactga tctaaaaata aaaaaacgcc
      841 cttaaatcaa gcgtgatatt aaaaaaaata aaatgctcct aaaaatccaa ctccacgctc
      901 cggcgcgtat tttcataaat accgatactg gtaaaaataa tggggttaaa atggtcataa
      961 tttaccacat aagagctgcc ggtcgacaaa gtagtggtac cgaaaatatt gtcctgcccc
     1021 agttggctca aatcatcaaa ggcgttagtt ttacggaaat catacaaaag tctttccgcc
     1081 cccgcttcgg ccgcaaaata agctcgcgtc gattgcgatt gaacgcctcc catctttaaa
     1141 ccggaaacaa caatagtggc gctgctaaaa ataatcaaca tcatcgcgct gaggataaag
     1201 attgtcagca taatagcgct accgcgctgg tgcttataaa gttttataat catatcaatc
     1261 actgcgataa taacgggagg cgatcgtggt ttggacgcgc atctcggaaa catcggcttc
     1321 acgccctaaa gacctggcgc ttaaatttat agaaacatag gcttgagcgt cgtcgtcttc
     1381 atgaacgata aaatgtaaat catcgatatt aatctgagcg ggggagatga aggcggagtc
     1441 aaaaccgcgt tccactttaa accgaatgac cccgttatca tcgcttaaat aataggccac
     1501 acactgatcg tgataatttt tcaaatataa aacatcgccg taagcattcg tgctagtcgc
     1561 gaaacgacga ttggccggca aatgggcgca accgccagcg gcgcgcttgg ccattctgat
     1621 ttctttggag ataacctcaa aaaaatattt aagattttcc tgaacattct gagtagcaat
     1681 agcttcgcgc tggctattta aaatcaagcg aaaaatatcc gtcattgata aaagaataac
     1741 aacaaacaaa gacgtggaaa taattacttc tataagagaa ataccgcgat gatttttgta
     1801 gcgtttcttc agtgctggca tattaatcaa taacttcgac caggcctaaa aaattaaccc
     1861 tcaccgtctt ggtggtatta ttaaggcttt cccgaagacg aatatccaaa aaagtactgg
     1921 tagcgctgcc gctttccaga cgggtaatgg gatcgggggg taaaaaagtg acattcgctt
     1981 gactaacctc gacattgccc aaactaagac tgtcaatact aatattgggg gataaagaaa
     2041 taacattacc gcctaaggcg gggatggcct cgccgttatc atattttcta ttatcgtttt
     2101 cgtcggcaaa aataaaatag gaatgattgc taccactgcc tgaagacaca aagacgcccc
     2161 agcctccggg cggaatttcc ccgtcatatt ttaccaagcc caaagcattg gcttgagcga
     2221 agcggatatt ggtgactaac gtttgcgcgg acatggttaa atcggttctc tggttggtac
     2281 tatgataatt cactaaaaac aaagcggtta aaatagaaat aatactcaaa ctaaccacta
     2341 attcaattaa ggtaaaaccg gtcttcaaac tgactttcct gtctttattt ccaatcataa
     2401 agttcggtct cgacctcgta ggtaaagact ttatcacgat cattccaagt aatcgtcgcc
     2461 tttaccgagg cggaagaagt ggcggcatta atcgatatta agcgtttaaa attactccgc
     2521 cgggaattag cggccactgt taccggcgaa tagtaccaat tatcgttatt aaaataaagg
     2581 ggacaatcat tgagactgga agcaacgcgc aaagtgggcg acaaataatc aacgcaataa
     2641 tcaccggaat taataccgtc tttccagttt ttgccctgca gccaattagt atcccgtatt
     2701 tgacgaacaa tttctatgcc ctcttgcgct aattgatagg cgataatact gccgcgatta
     2761 atagtttgcg cctggatgct ctgcaccgcc aaattagcga caccaatcat ggccagcgag
     2821 acaataagca acaccgccat tatttcaata atgctaaagg cgggatgaca ccggctaatt
     2881 gtgcgcaatc gcttaaacat aggacattct tattccgcct tcacttccct ccaaagaggt
     2941 aattggaaat taatcgtcaa gaccggcgaa gaggtcgaac aagtatagag atcggggtca
     3001 gttatctgta aagacacgga cttcggattt ttattaataa aaacaatagt agtagtcgac
     3061 tgcgtcgcct gagaaatata agcagccccg tcgcttactt gccaccaaaa ttgacagaca
     3121 ccgtcaacgc ataattgtgg gttggaatta ggtgaagcaa cggtataata acgagaatta
     3181 ctgacaaaat cggtgctgct gccgacaatg atttcagtcg ggctccagct aaaataagga
     3241 gtgggaaatt cgtgcttata gttagtaaag gtcaggtggg gatctacggg attattaacg
     3301 ctattggcgg cgatattatc agttaaaacc ccgaaagtat tggtatcaaa ggggaataat
     3361 tctgtgggtt ggttattttc gtcccacaac tgcagccacc aataatagga agtgccgtaa
     3421 gcgggctgga aactacaatt aggtccgggg caaaccaact gggaagcaaa gccggcaatc
     3481 tgaccggaat cataaaccgg gctagaagta ctattggtag tattaacaac cacccgataa
     3541 gcggtttgga tgccgccgcg acgccaccat ttaaaggtta cccgccgcgc cccctgactg
     3601 caggcatcgc taaaactcca attgggcgcc gacatctttt tcaaatctaa tttccaaaga
     3661 taaactttat ggtcaaacgt gccgcaacta ccattattgg cgcaattaaa cataatggcg
     3721 ccaaaggaac tggaagcata gccagagaag attcctggct gaggggtaag ataattagtc
     3781 atcgtcgtct ccggcatgat ggcgctattt aactcgatcc attcgtcggt accgataaca
     3841 taggcccagc caaaaactct ttcattctca tcgttgtagc aagcaataca gttattgctc
     3901 gcattacaag gctgctgaca attaacatta aaatcgtaat taggggcagc cggggtactg
     3961 gtagaaccaa aatcaataaa ccctaaaacc ggattccagg ccaaaccaga aaaagtatta
     4021 tcgctgttta aattaacgcc atgctgggaa aaagagcaag gaggaacatt aaaacgaaaa
     4081 ggaaaagtaa agggaaaacg gccggcaaaa tcatcatcca agcagttaaa actgatatat
     4141 tcccgagaag agctggctaa gccacgaata ttttcattaa tcgaagcgtg cgccacctcc
     4201 aaagacgacg gcagtaataa agccgcaaac ggcaataata aaaacaaaaa atggcggaga
     4261 cgcatattta taaattctct ttgattatag catttttcac caattctttc attattctct
     4321 ctcctttaga gatcatcgct aaacgttcct tttcagtcgc gtcgtcaaaa ccggccaaat
     4381 gcagcaaacc gtgaatcatt aagaataata aaatataagt aaatgatttt tgggcaccga
     4441 agactgctaa atatttttgc ggacggcggc agtcggataa attaataaag atctcgccta
     4501 aagcgccggg agaaatcacc ggcgcgcgga aagaaagaac gtcggtcggc ttatctttcc
     4561 ccagaaaagc ttgatttaat ctctgcaaac gacggtcgcc agccaaaact aaagacagct
     4621 ctccgtccag atgtaaacgc tgagcggtaa attcggccgc gcgccgtaag actttcaagc
     4681 ccaccggacg cttgcttaaa tttttaattt ctatggaaaa catattaagg acgtaatttt
     4741 ttgactattt tggataatag agaataaata aaatatttat gtttagaaat aaccaagaga
     4801 aaagaaccgg ggtacactcg ctcttccccg ccaaaaccgc gcttaaaacg ggttaccccc
     4861 ggccaccttt tttcatcaat gccataaaaa tcataataac ggcaatgttt ggctttagcc
     4921 ctttgtatca tccgccattg taaaagatag ggggccatat actgccggtc gttattagca
     4981 gaggcgccat gaaggtagac cgcccgacca ccatagaacg aaaacaagcc ggcggccaaa
     5041 attttacctt caagccgggc aaagtaaagt tcaatcgcgc cgccgccctt ctctaaaaga
     5101 tggcgataat gagccaaaga atgaccgcga aaaccatcgc gtgccgttgt ggtctgtaat
     5161 aattgccaaa aatcgaccaa atcttcagac tgacccgaaa caatttccac gcctttcttc
     5221 tcggccagac gaatattata tctagttttg ggatgcatct gcgataatag ctcctcctca
     5281 ctatagctta aatccaaaaa caaacttttt tcaggttgaa tcgaggtgat tttttgccca
     5341 aaggaaacaa cgggtaaagg ccagttttcc ggttcaaaac ggattgacat taccccttgg
     5401 cgcttggctt cttgaattaa atcggctaaa actaagggcc aggcaatctc ggcgccctca
     5461 gacaaaagcc aaaccggtcc ccgtggtaag taccatgact taaggccaaa acgattagtc
     5521 gttaacacct gcgctaaaac tgctaccatc cccttttcct gaagccagga aaaacgttta
     5581 acctcacgct tctccgctaa agcaatctgc cgccaaatac ccgactgtaa gagttctccg
     5641 ccatccggca aagactgcaa actggcttcc caagttaaat cggccaaatt gtcttcttta
     5701 gttaaaatca taacctaatt atagcaaagg ccggcctaaa aacaaaagcg cccctatcaa
     5761 ggagcgctaa tataatattt attaaattgt aaactactga ataccagctg gtgtcgcggt
     5821 atgagaaccg gcgggaacat taccagtagc ggaaccgaga caataagtta aagcatagga
     5881 ggcggtgcta ccacttccag agtaagtgta agtgtatgtg ttctcagtat catcacaggt
     5941 accgtcgacc gggagggggg cggttggtat taccttaatc aaaggcggag tggtggtagt
     6001 taattgatcc aaaatatttg gataattatt attatcatta taatacattt ccaaagcagt
     6061 ttggatttgt ttgatatcag agatacgctt agcgtcacgg gctctagcgc gagcgctgtt
     6121 aagagctaaa accgacatag tcgagagtaa gccgatgatg gcgataacca ccaataattc
     6181 aattaaagta aaaccttttt tgttattcat agattcaccc cccttccaaa aatttccatt
     6241 tacgccctta ataccggtag taaaagcgta aacgcaaata aattttaaga cattaaaatt
     6301 atagcaaaat cctataatcg gcacaagagt atcacatgta agctaaaagc aaaaacaata
     6361 atcggctttt aaaactgatt agccatatta tacatgggca ttaaaatagc ggccaccata
     6421 ataccgacgc cgacgcccat aataaccata atgatcggct ccatcagcgt gctgagattg
     6481 gccaacatcg cactcgactc gcgaccgtaa aaatcagtaa tcttatccag caccgaatcg
     6541 atacgccctg tccgttcgcc gacactaagc atttgcggca ccattttcgg cacatcgtta
     6601 cttgcctcca ttaccgtaga tagtggattg ccgtcattaa tggattctaa agtggctaaa
     6661 atcaaatcgc gataaacaac attacccaca acgttagcgg tgatttccaa agccctggtg
     6721 atagtcaccc cgcctttaag caacgtggat aaaatacggg aaaagcgcac caaatagata
     6781 tatttgaata aacgaccaaa aattggcatc tttaatttaa aagtatccag ttgataacga
     6841 ccaacttccg tgcgcagata ataacggacg gcgataatta aaccgactaa tgtcagaatt
     6901 aacagaatca aataattttg caagaaatta gaaatagcga tgataattct ggtagccagg
     6961 ggtaattgca tgccggtttc cgtcaacata ctggtcaggt taggaataac gtaaaccatt
     7021 aaaattacag cgacggccaa caaaccaacg agaatgaaag cgggatatat cattgcccct
     7081 ttgattttag aggccatatc atagctcttt tcggtttcat cagccaaata tgttaagacc
     7141 tcatccagtt tgcccgaagt ttcgccgctc ttaatgatat taacaaagaa ttcggaaaaa
     7201 attttcggac gtttggcaaa agcgtcagaa agcaaagccc cgctatcgac ctcaaaggca
     7261 atatcagcaa tcatcttctt taaagaaaaa ttattggtct ggtcaattaa tattaaaagc
     7321 gattcgacca cgggcacgtt agcggcaatc atcaccgaaa actgacgaga aaaaattacc
     7381 aaatctttga ctttaatcga atttaaaaac aaattaattt ttgcctccag gctgtctgtt
     7441 ttgtcggcaa tagccaacac gctaaaattt tgccggctta agcgcttgac gacatccgct
     7501 tcattaatgc caacggtaag ctccgatttg gtcttgccgg tgatattatt tttagccttg
     7561 tatttaaaaa tcggcataaa ttaataaaaa aattcaacta actttcaata acgcgcaaaa
     7621 tttcttccat ggtcgtaatg cccttcagca ccttgataat cccatcttga ttaatggaaa
     7681 taaagtcctg actggctttg accgcgtcga tatctaaatt cttatccccg cgattaatca
     7741 tttctttcag ttggtcatta atatcaatta cctcggaaat agcgacgcgt ccggtaaaac
     7801 cggtattttc gcaatgactg caaccgaccg aacgataaaa ggtataattg ctcaaattat
     7861 taaggtcctg taaatctggc aattctgttt ttaaaatatg agccggcaca tctttgagag
     7921 ccgccgacat tgcagccaat tccgattcag gtaaagtcac gacttgtcgg catttatcgc
     7981 acaaacgtcg cgctaaacgc tgagccacga cagtttttag ggtagaggct aaaagaaagc
     8041 gttcaatctt catgtctaaa agacggaaaa ctgcccccaa ggcgtcattg gtgtgcaagg
     8101 tggaaataac taaatgaccg gtcaaggagg catgaatgga taattccgcc gtctcctcat
     8161 cgcggatttc gccgaccatg ataatattgg ggtcttggcg caaaagcgaa cgcaaaccgt
     8221 tggcgaaagt aaagccgact ttgggtcgga cctgagactg attaacgcct tttatttcat
     8281 attcaatcgg gtcttccagg gtagaaatat ttacaccctc ctggttaaga atgtttaata
     8341 aggaatataa agtggtggtc ttaccagaac cagtcggacc agttactaaa ataataccgc
     8401 tagtcttctt gatcgccgca ttgatcgccc gaagagaatt agaattaaag cctaaatcag
     8461 ataaagccgg cgcgcctttg gccgtatcta aaatacgcat caccactttt tcatgattag
     8521 ccagaggcag cgtagaaata cgaaaatcaa ccgcgcggcc attgaccaac aaacggacac
     8581 ggccgtcttg aggcacgcgt gtctcgtcca gcttcaattt ggacataacc ttgatgcggg
     8641 caataatggc attgtggata cttttgggca aggtcaaaga agtgtgtaaa atgccgtcga
     8701 tgcggtagcg cacccggctt tccttgtcat acggttcgat gtggatatca ctggcgcgcg
     8761 cttcgacggc gtgacggatg atgacgaaaa cgatgcgaga caccggcgcg ctattaatat
     8821 cctcttcgtc aaccatcgcg gcgtcttcgg attttatcgc gacgacgtcg tcactgtctt
     8881 ccttggcctt aatctctaga gcgctggaaa tttcttcttc gattttctga tactgcttga
     8941 aaattttttg ccaactgcta gccgataaaa gatagtactg gacgtttaat ttttcatcca
     9001 gggccaaaaa attaacagct tccatcgctt tcaaattggg ctcaaccaaa cctattttta
     9061 aagttttatt ttccttggct aaacaaacaa tattataagt gcgggcaatt tcttctggta
     9121 aaaaatttaa aacctctttc gggataactt cgtcggtgta agcgaaatag ggcaaattat
     9181 ataaaaccgc cttagcttca gttaattgct cttcattgac cagcttgttg ttcaataaaa
     9241 aatcaggcag ctctgtttta gcggccgcaa ctttaagctt gatatcggca acttggtcgg
     9301 caacaagcaa ttttttctcc tgcaatatct tcagcaactg ctcttgttga tcaataagca
     9361 taaaattatc taaaacgtct aattctcctt cttctctttt accgggaaaa gatgagaatt
     9421 gcccaaatag accgccggcc aaagcggcgc ctgtaaatta ggattggcgg cccgatttaa
     9481 caccggtttt cccaccttat caaagtcaaa ataaagcacg tgattactga ggttgttaaa
     9541 tgtttgatta tttagtattt taatatctaa attaccggcc tccgaaacat ttaagaccgt
     9601 ctctgaggaa tcgacagcgg cgccgaaaac tcgagcgctc aaataagaca acagtctttc
     9661 cctaaaagca aaaattaaaa ggccgacaat aattaaggcg ccggtaagta tataaatact
     9721 ggaactgcgg cttttagaca taagacttta tttgaaataa tatgtataaa gagtaatctc
     9781 ggccgttttt tcttcttggt caaaattaat ggcatcaatg tcgattaagc gcagattact
     9841 ctcaaataat gacaaaaggt tttttaaact agcgtaatcg atgcttgcca gattgataat
     9901 agccataatc acgccgacgt gctcagaatt gggaatagcc agccgttgtt catctttagc
     9961 ggccagcggt tctgcttcac tatccacttc atcaatcttt tctaaattaa tcccggtaat
    10021 taacaaacct tgctgggtaa tgatatcttc cagctcgcca aataactttt ctttagcgta
    10081 gttatcgggc aaaacctctt taatcttgta aatatcgcta tcgctaattt tgtgatacat
    10141 cgcggcggcg gcttgcaaat caattaattt ttgccgctga cgctgataga gctgctcttg
    10201 ctgactgata tcgtctttaa tagcaaccaa agttagttca aacttaggtt taataatcag
    10261 aaaataagcg gctgccaaaa aaattattac cgctaaaacg accaaaatat taaaattggt
    10321 atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccaaaata ttaaaattgg
    10441 tattaagaaa aacattaatt ttgttcttat tcttatgttg tttagcggcc acagccataa
    10501 aattaattag cggcagaatt tttaaatata ttcggatcga caactaaatc cagattgaat
    10561 tttatatttt caacctctgc ttccgcgccg ccgctatcgg tcgggcgttc gctttcaccc
    10621 ttattaacat ggacggaaat aaccgccggg ctctccagca tcgcccgtgt ctgccagctg
    10681 atatcccgga aagttttggc ggaagcgtct aaagaatata cgccgctttt atccccctga
    10741 aaaccgagat aactaacact gcttaaagta ttcttttcca accagttaaa aaaattagtc
    10801 caataaatat ggttctccag caaagtatta gctaaatcgg ctctttgctt aaaagctaaa
    10861 atttgatcag attcagtctt gagcgcttta atttccttgg acacctgatt aaatttagtt
    10921 tcagcggcca acatgcggac attttcataa tcagaccacc agttaagacc aagataaact
    10981 tccgctacaa ataaagcggc aatcaccaaa gcgaagagga gggtgccgat attcttgcca
    11041 acctcaaaat caaccggcat ttcgctctta atcaggttca cttccaaaac tttagggttt
    11101 tgcgaggaac tgaccactgt cttgcttttc ttttttggtt ttatagaaaa attaaacaca
    11161 tcgtaaaagt taagaaattt ctcttaaggc caggccggcg gcgacggcca attttggtcc
    11221 cacttcggcg attatcggct caatttctga aggataaata atgcggctga aaggattacc
    11281 gataatcacc tgaagattta ggcggcggga aagataatcg gctaaattca taagcagcgc
    11341 cccgccccct gataaaatta ttttctcaat tttttgaccg ccggcattat tctgataaaa
    11401 ttcccgcata tactcaattt catggataac cggcgctaat gtcttgataa ttaattgcgg
    11461 caattcctct tgagaaacat ccgaaagcga agcgcccaaa tcaaacttga attgctctgc
    11521 ctgattaaaa ctcaaaccca atttttccgc caaaacggca gtaaccgtgc tgccgcaaat
    11581 ttccaggcta cgattaagca cggggatgct ctcgtgcacc actgacaaat cggtgctatt
    11641 ggcgccaatt tctacaatca tcacagtaga aggatcatta cccatcaaag ccctaactaa
    11701 agaaaaagtc tcagtttcca aactggccaa ttctaatttg gctaaacgaa aaatctcaat
    11761 ataacgccgc accaacttct tcggggaacc ggtaagaaaa acgcgcatcc cctctttatt
    11821 cccatccttg cccgtcggca ccaatttcca atctaaaatc atttcttcga ccggcaaagg
    11881 gataaccttc ttcgcttctt ctgccaccgc cgcatccata tttttcttgt cggtaggcgg
    11941 caaattgata attgaagaaa aaaccgaaaa agtcggcaag gaagctgtta ctctcttcgc
    12001 tttaatgccc gctttcgctt tcacctttaa aatagcctga gcgagataat taatatcatc
    12061 tattttggtg aaattaacgc cgctgacatt ttcactgaaa gcgtagttag aaagaaaaat
    12121 ctttttattt ttctttttta actccaccat tttcaaagaa gaatcaccaa tgtctatgcc
    12181 taaaaataaa ttgtcacccg ccgaggaaaa taaacccata gtaaataaaa attaacgtca
    12241 aaaacccaaa tacaaaaatt gtgttctctc cgcttttagt atataatatt taggagatgg
    12301 cgacaaggaa attctcaaaa tctgcagatt tcctccaagc gtcattattt tctattgtat
    12361 cacaaaaaga taaaattaaa aaataagata tgggattttt tagtaaatat aaaaaaatct
    12421 ggctattgct cggatttatt gccttcgttg gcctgatgat ttatcttatt tggattacct
    12481 ttttccgtaa tccccttact cccccggcgg tcaccgagat caccggcaac atcggcggtc
    12541 ttaatccggc tgatgaaggc agtggtaatt attcgtccac cagcggggac gggcgtttaa
    12601 acgtaagcga agacaatcct agtaacagac aaaataacgg ccaaattagc acccctgctc
    12661 ccaccggcac ggaaatttca gacagagccc tgggtggcat cactaaagtc actgccctta
    12721 cctccgaccc ggtgctcagc ccaaccatga ccagtaatga cgaattaaga tattacaacc
    12781 aaaacgacgg taaattttat aagctggaca gtaacgggga gttggtgaca attagtgata
    12841 aaatctttca tcaggtaagc aaggtcactt ggtcgactga cggcaataag gcaattttag
    12901 agtatccgga cggatcaaaa attacttacg acttccaaac tcagaaacaa gtcaccctgc
    12961 ccaaacattg ggaagacttt tctttttctc ccagtggcaa gcaaatcagc gctaaaagta
    13021 taggcaatga cgtggaaaac agatatctaa tcaccgctaa cgccgacggt tccaaggctg
    13081 tttccttaag cgccatcggg gaaaatgccg attatgtcta tccggtctgg tctcccaaca
    13141 atcaagcggc cgccatgtac actcggggcc tggactttaa ccgccaagaa ctattctttg
    13201 tcggactaaa cggtgaaaat tttaaatcaa cgataattaa cggccgcggc tttgaaccaa
    13261 aatggtcgga tgaaggcgac aaactcctct atagcgtcta tagcagtgat aataatatga
    13321 acccgagcct ttggattgtt aacgcgcaag gagacaacat cggtcgcaac cgcaccaatc
    13381 tcggactaag cacctgggcg agcaaatgta cttttgccag caacgaggaa atctattgcg
    13441 ccgtcccgga aaatctaccc caaggcgccg gcctcttccc ggaactagcc gacaagacta
    13501 cggataatct ctacaagatt gaccttaata ccggggctaa aaaattaatc gccgtccctt
    13561 caaccgccgg taacatctca aacattatgg tcgactcgaa tcaaaaaaca ctatacttta
    13621 ccgacaaaaa cacgggaaag atttacgaaa taaaattata ataatagcaa aataaaaaag
    13681 aatcggcgcc aaagccgatt tttttgtgat ctatgtttaa aaaagtcatc aacttgttat
    13741 taaaaataga aaattttatt ctacctgaaa cctgtctgaa ttgtcgacga gcaggacaag
    13801 cgctttgccc cgcttgtcgc gtccaaatca aaaaaattcc ggaacgttgc ccgacttgcg
    13861 gtcgccggca aaatctaggt cttttttgcc aaaagtgccg gcaaactcat cgaactataa
    13921 gcttcgacgg tatttttgcc tatacttctg aagacgacca aattatgatc aaagcgatta
    13981 aagctctaaa atatcaaggc gtaaaatctt taggaagaat cttaggtaaa atgctcggac
    14041 ggcgcttagt catctcctgg aaacaagcgc cagcctcaat aaaaaaacga aacaccataa
    14101 tcattccgat gccgctccat ccccgccgcc aacgcgcccg tggctttaac cactcccaac
    14161 tgattgccgc tggcattgcg caagtaagtg gctggctaat taatgagaac ttgaaacgta
    14221 aagcttggca caagccaaac gcggtctccc tcgataatca aaaaactttt gtctataaag
    14281 gcaaattatt aaccggcaat aacatcattt tggtggatga tgctattgcc accggcgcta
    14341 ccgctcaagc ggcggcgcag gctttaaaaa cagccggagc agacaccgta atcgtggcca
    14401 ccttaacgca aagcttttaa aaagccttta caaacagaaa aaataagaat aaaattacaa
    14461 atgaccgctt gattgtcgag ccaaccaaac tcgagagcta tcgccgccga ataggcggcc
    14521 taaaagacgg tcccaaaaag cgctccccct cgggagaagc tgggtggaac cgcgagacct
    14581 ttttcgtccc gtcccggcaa aaacagattg ttctgttttt gtgaggatgg gattttttta
    14641 aattaaaatt ttattaatac aaacatatgt ctactctgga aacgaaacgc cactcattgg
    14701 ctcatttaat ggccgcggcc gtcaaagaaa tgtggccaca ggctaagtta gccatcggtc
    14761 cagccatcga taatggcttt tactatgata ttgattttgc cggggccagc atcaacgaca
    14821 ctcaacttaa agaaattgaa aaaaaaatga cgcatctggt caaacaaaat ttagaattca
    14881 cccgcaccga aaaaaatatc gaggaagcct tggcttgggc gcgggcagaa aaggacccct
    14941 acaaagaaga aattatcagc gacctcaaag aaaaagggga aacaaccgtt agtttttata
    15001 ctgtcggcca atttaccgat ctctgccgcg gtccccatgt cgaaaatacc aaagaaatta
    15061 aacctcacag ttttaaacta gacaaactag ccggcgctta ctggcgggga gatgaaaaaa
    15121 ataagatgct cacccgcctt tacggtctcg ccttcgacac caaagaagaa cttgatgaat
    15181 atcttaaaat gatggcggaa gcggagaagc gtgaccaccg ccaattaggc aaagacctgg
    15241 atttgtttat gaatcacgaa ctcgctccgg gcatgccttt ctttctgcct aaaggcatga
    15301 ttcttcttca ggaactgtta aaatttgtcc gttcctattc ctatggtgac ggctattatg
    15361 aagtcagaac gccccagata ttaaatgcgg aactttggaa aatttccggc cattgggaac
    15421 attatcaaga ggacatgttc tgcctgcatc acgctgaaga cgatattgac ttaggaatca
    15481 aaccgatgaa ttgcccggca catatgctta tttttaaacg cgatttacac tcatacaaag
    15541 aattgccctt gcgtttggca gaaaccacca ccctctatcg caacgaaaaa tcaggtactc
    15601 tgcacggcct gaccagagtg cgtagccttt ctcaagatga ttctcatatt tttgcccgcc
    15661 ccgatcaaat tcttgacgaa atctctaccc tcttagacaa aattaaaatg atttatcggg
    15721 tcttcgactt agagatagac gaaattcacc tctccactag accagcacat ttcttgggcg
    15781 aaaaatccgt ctgggacgaa gccgaggcca atttgaaaca agccttagaa aaagctaagc
    15841 tggcatatca tatcaatgaa ggcgacggcg ccttctacgg ccccaaaatc gatgtcaaag
    15901 tcaaggatgc catcggccgc caatggcaat tagccactat ccaactcgac ttccaattgc
    15961 cccagcgctt tgccttgaaa tacaccgagg ctgacggttc cgccaagaca ccggtcgtca
    16021 tccatcgcgc cctcttaggc tcaatggaaa gattcttggg agtcatcatt gaacattatg
    16081 ccggcgcctt tccgctctgg ctatcacccg ttcaagtaaa attaatttcc gtagctaaga
    16141 cccatctgcc ggcttgccaa aaattagcca acgagcttaa agaagctggc attagagtag
    16201 aaattgatga aaataacgag actgtcggca ataagattcg taaagccata aaggaaaaag
    16261 tgccctatat gttagtgatt ggcgataaag aagcggcggc ggagaaacta gtcgtccgcg
    16321 accgcggcgc caaggaaacc agagaaatta gccgtgaaga tttccttaaa gaactgacgg
    16381 ataaaattaa aaattacact taaaaatatc aaacaaaaaa ccggcgaaaa tttcctttcc
    16441 gcctgttttt atgataaaat tcaaatacta taaatttaaa ttttataaga tatgaataaa
    16501 aagaaaagtg ccttaagcgc cattattatc ttattggtct tagtggtggt tgtttcaatt
    16561 tttatctcca agcgccaaac cggcactgaa caaaagacaa gtagcgataa cggcccaatt
    16621 cttttctaca gcctatcctg ccctcattgc caaaacgtag aaaaatatat ccaagagaat
    16681 aatattaaaa ataaatatag cttttctcaa ctagaagttt ccgctaataa gcaaaacagg
    16741 gaaatactag tcgccaaagc taaaatttgc ggtctggata cgcaaagcct aggcgtcccc
    16801 ttcctttgga ccggagaaaa atgcttgctg ggcgatactg acatcattga ttttttcagc
    16861 aaataaactt atgaagaaaa aatatttatt cgctcttact ctggcttttg tcttgaaagc
    16921 cctgcccgcc aaagcggtct gtccggtctg cctcgtcgcc gccggcgccg gcttgggctt
    16981 atccgaatat ctgggcatcg acgatagcat tgccggtctt tggatagggg gattactcat
    17041 cggtttgagt gcttggacca ttacttattt caataaaaaa aactggctaa aaaattacag
    17101 cgcctggcgc gaccccctaa taatcatcgt ttattactta atggttatct ggccgctcta
    17161 ttctcaagga tttatcggca atcctttcaa ccgcttatac ggcgtagata aattggcctt
    17221 gggtattact gtcggcagta tcttttttgc tctggccgcc tggtcttacg agctaatgaa
    17281 gaaaaaaaat ggcggtcacg ctcatttccc ttttgaaaaa gtcgttttgc ccataagcac
    17341 cctggtaatt ttaagtgtta tcttttattt tttaagcaga taaatatatg gacctaagca
    17401 aaaaccctat caaaagcctg caagaattca tcaacaaggc ggatgatatg aaaaagcagg
    17461 aaaagatgga tttatcctcc gaccaagacc tgactatcgc cattatgaac ctggtcagca
    17521 ttgaggaaca tttcttcttt accggcgcca agaccggcaa aaacgaatat tacgacttga
    17581 ttaatgaagt tcgagaaata cgcaaggaat tattaaaaaa aatcataaaa gaatatgagg
    17641 gcgaaatctg gtgtatttcc aagcatctgc tggccgccag ctaccgcttg atggaagtcg
    17701 gcaccaaaca actgagccgc gggcagaaag aggaagctta ttcgctgttc gataaagcct
    17761 accagctcta ttctctcttc tggggtctca acctgaaact tatcggcact ggtgatatca
    17821 aaaaaattga cgaaaaagcg attgacaagc acgatgaaga aaagaaaggt tttactgaca
    17881 aactaggaga actggtaaaa aaagtaatta actgctgcct tgaataaaac taaatttata
    17941 caattagaaa agaggtcgta agcggcctct ttttgtcaat taaactctac taattattaa
    18001 ttatttttta atctgaaaaa atctttagga actcccttgt atttaatgag ttcctctctc
    18061 tgtctttctg aaagatttag tttggccaaa tcaattacta gcctggtatc agggttgtca
    18121 accaggataa tctcaatgag ccataagccc ggaatcactt gttccagggt ctcagagaaa
    18181 tacatcacca ccgcatgggg gtgaaataat ttacggactt ccgggacttt aaaaatatgt
    18241 cccggcaaaa tggtggcgaa atttgattcg cccaccacga tgttaccaac gccatttttc
    18301 ttttccatct gcttttggtt ttgtgtctcc aggggagacg gtttataaat tatttaaaga
    18361 actgttttaa tttgataaaa aaatattaat acaaacaata aattttgtaa agggtaggat
    18421 taaatcacta tttttctact aataatacct ttttttcaac accaaccaag ggctgatgat
    18481 aaccgctggg ataggcgcga attgccggaa agagaatttc agatgaataa cggtagctag
    18541 cgccacggcg cgtacccgga tcattggtaa taaattcgtc cgttttgttg tcataaccga
    18601 taatcacgac caaatgcctt tccggaccag gcgctttaaa attaggatta tgcaaagcct
    18661 ggccgttaac tggcgccaaa atcaatttac cctcttttag agccgagatt agatcgttct
    18721 tatcgataat cacttgagtt ttcaccttat cgtaatgaaa ataatcttga aaaatacgga
    18781 cggcgacgtc atcgatataa acatcgcgat gctcgccgta tttttcctgc tgccagtcgg
    18841 ctaaggcaat aattgctttt tccgacgctt tgggactagt atctatttcg ccccgcgccc
    18901 aagccattgc catcaaagcg ccggcttctt cgcaaccatc ctgctggcgc gcgtcctccc
    18961 aattggctaa aggggcttga gaaaggaaag ggacggacaa aataatattt tcattgccgt
    19021 taatcaaagc gtgggaagag gcgaggggcg cggcggcttt aattttaata tcagcttcta
    19081 aaacattttc tcttttttcc ttttcatcca aagaagcgac ttctcgttcc ggcaagccaa
    19141 tactggccgt cggcaaaacc tcggctgtta ccggaacaga ctcgttattt tcacttcccg
    19201 gctggccttt gtccattagg aaaaaaattg ccgccgcgat gattatcaaa aagaaaaaaa
    19261 tctttttcat cgtctttatt taatcgccgc cgccaccgcc gcggcgatac cgggagtaaa
    19321 tggctccggt aaaattctgt caacccgggg aacagagact aaagcggcca aattctccgc
    19381 cgcctttacc agcctctcat cgctgatctg gcgcacctta ttatccaaag cgccgcgaaa
    19441 gacgccagga aaaatcagca cgttattaat ctgattagga aaatcactgc ggccgctggc
    19501 cacaataaaa gcgccggcag ctttggcttc atccggcata atttccggca ccggattagc
    19561 caaggcaaac accaatgatt ttggcgccat tgtcctgacc atttcccggg taagcaaacc
    19621 ggctgaagaa acgccgataa aaacatccgc tccaatcaaa acctcagcca gcgcgccttt
    19681 ttgtttgccc agattggtga aggtggctaa atcggctttt tccttgtcta aatcgggacg
    19741 gccgacataa atcgcgcctt gacgatcaca agcgataata ttttttacac cataaccaaa
    19801 caaaagacgg gcaatcgcat tgccggcagc gccagcgcca ctgataacaa ctttaatatc
    19861 cgaccgatca ctacctctta acttcaaagc attgattaag gccgctaaaa ctaccgccgc
    19921 tgtgccgtgc tggtcgtcgt gcatcaccgg aatatcgagt tccatctgca aacggcgctc
    19981 aatggtaaaa cagcggggag cggcaatatc ttccaaatta ataccgccaa aaaccggcgc
    20041 caaatatttt acggttttga ttatttcttc cgtatcttgg gtatccagac aaatcggaaa
    20101 agcgtcaaca ccggcaaatt ttttaaacaa agcgcatttc ccttccatca ccggcaaagc
    20161 cgctaccgac ccgatattgc ctaaacccaa aactgcgcta ccatcgctca cgaccgcaat
    20221 cgtgcgtcct ttaattgtat gcgtccgcgc cgcttcctta tcggcggcca ccgcctgaca
    20281 aaccgccgcc actcccggtg tataggccaa agacaaatcg tgttggttgt ttaaagggat
    20341 agataaagcc gtagcaattt tacccccgta aaaacggtgt aattctaaag attcggcatt
    20401 taaatcagac atatagagag aaccggctct tttgaagaaa ccggagataa ttattaagtc
    20461 aattaatcat aacaagaata tgagaaaaat tcaaaggcgc gccgaaactg aaaatctacc
    20521 cttatcacta atctgtaaat atttgtgata tgagaaaatt tatcgtcaaa aaaggggaaa
    20581 gagccaagag acgtttaaaa ataatcactc ttctttaacg ggaaaagcgc cgctgggaaa
    20641 atggacacaa gttgctgcca tttggcgcaa tgatagcgtc gacttatatg ttaacggggc
    20701 tttaaaaaat actgtagcta tcaataatgc cccaggaagt aatggccagc aataaaagga
    20761 aaattaaaac caagacggac cgcttttagc agtccgtttc ttaactctta atttttatcg
    20821 aaaatttcta ctgcatcaac acgccttcaa tgactgcgcc acctccgtct tcaatcatta
    20881 attccgaaga ctcaacggca ctatcaaggg cattgtccgc agcgctggca gagcaggctt
    20941 tcaagtcgga ctgataagcc ttccagatta tctgttgctc gtctttggat tgtttaatta
    21001 tctcttgata cttcgcctga aaatctttaa tgcaaatatt taaattttcc aactggcccg
    21061 cggtggtctg aattgccttt ttctgacatt cgccgcgagt ggaaataata gcttttaaag
    21121 actcggcgct taaatcaatt tggttctgca aagccttatc ttttttatca atggccgcgg
    21181 aaacgcaagc aatcatctcc gccgttacta agcgcggctt gggcattggc ttgatggcct
    21241 gatcggtggc tttagggccg gcattatcga ctgatttagt ttcttttttt accccccata
    21301 aagaattgcc tattttttga atcttctcat aaagattaat aaactgaggg gctaaaatct
    21361 tctccaattg agctttattg gtgtttgttt tctcttgatt atgagaacta atttgctctg
    21421 ttgcctgatt cttgacggcc gtggacaggc gaatgccgta taaagaaccg ttctcttttt
    21481 taattacttt aaaatactta atctgatcgg gagaagggat cttttctaaa gaaccagcgg
    21541 ccgtgctagt agcaatcgct gtgacggaga cggcactatc ttcagctctg gccgacgcta
    21601 agccgactac agccacacca attaccgtta atacagcaaa agtaatgatt tttttcatat
    21661 gatttaaatt atttataatg tcttgctttg ttaaaattat aacactactt aagaaaaaga
    21721 ggaatgcgtg cgaaaccaaa ctatttatta ggctcttggc tctttacttc ctcaattggt
    21781 tttttaataa agaccgcctt gtctttttct tggtctttat tattttggtc tgcgttagca
    21841 gccaagaaat caactccgac ctcaattttt tctaaaatac tgactatttt atttattttc
    21901 cagtaccaag ttaaaagctc tctaattaat aaccaaacaa ttatcagaaa taacagaaat
    21961 aagacaacca ggataattgc taataagtct gaatttataa aatcttttag tgtttttaat
    22021 aataaatcta gaatgttagg aaataatgaa ttcatatatt tatagtggtt caatttgtca
    22081 aacagtttta gaaccatgaa aaagttgatt tagtcgggac gatatttgaa ttaatatgta
    22141 aatttgtaaa ctgctggaag aagacggttt ttttgttttg tccgaaatat acttcggtcg
    22201 gagttttgtc attaagggac tggccctggt cgctattcat aatatccggg atagttattt
    22261 cataggttct ctccaaggct cttggacaaa aatcgatatc cagggtatta gacataaact
    22321 ttttagccaa aaacagggcg tagtagtgat ataaaagtta tgtatcaaaa atctgcggtg
    22381 acatgcccaa aaatacttgg tgtccgagaa agaggttctt atcgtcaagg taaataccga
    22441 tgatcttaga aacgctttcg ctttctaaat cattaatatt tttattttaa tacggattgg
    22501 taatttttca gcataaagat agcatccgct ttcaggtgat ttcggaccca gtctttctgc
    22561 ttctcgttat caatcaactc tcccaatccc tgcaacattt ggctgtcttt gatcttttcg
    22621 ataaaagcga tgcagtccgg caaatattct ttgaccgttt tgccggtaaa attctttata
    22681 acttcggcgt tgatatccca gtggttcttg gcaaaatact gaatatcaaa aatatctctc
    22741 atggctgttt cggttctcaa agtcaaagcc gctaatttac cggcgaatag ataatctttt
    22801 ttggccacca tcattgaaat acccagatat tctttcatct catagaggtc tttcatgttt
    22861 tccgccagtt ttctgacatt gatctcaact ttgacgttat gatcctcatc gccgtaggag
    22921 agcaatgcga agacagtaaa cctcttgatg tactgatctt taattgttcc gtacttgctt
    22981 aaaatggcga cgatcttttc aaaaaccatc ttttggtttt cctcggtcac ttccagtaaa
    23041 tcaaaatcca agtccaccga gaatctcggc aggttgtaga aaaagtaagt gcaggtgtcg
    23101 cctttgaatc ccaagagcgg actgattgag acatcggtat aaatgtcttt taaaacctgg
    23161 cccattatta actgatgttt ttctttgttt agcatatttt tattgagaat ttttcctata
    23221 cttattcaat ctctttatca atgatttgtt gttatatatt tttaccagct catcgcattt
    23281 ttcccagttg attcctttca ggttgtcgaa gtagtaattc ggcgataaat aaatcatgtc
    23341 caagaaggct ctttccggag tggcgatgct gtagttatcc ttgctctcga tgccggctga
    23401 attaaacagg accgtatctt tcagttttct gaaagtaata gaatatttat cgacagtcat
    23461 aattttcggc catttggtcg ctacaaagat tgtattgtaa tgctggaaaa tgacaccggc
    23521 ttctcttaag accgtttcga agctgatgta tgagggggta tagaggctgg tcgctagttc
    23581 ttttgcgttg aaatttttat ccttggcaaa tactcctctg gtcaggcgta tcaagacacc
    23641 ccgccttaca taatatgata ttttatcttt caacttaatc tgatcgtttt ccccccaaac
    23701 taaagccaga tccttgctgg tcaggatagt tttgtttgac tgatataatt ttgcgattaa
    23761 gctgtccata gtgagtttat tggctggaag ttggatattc tcacacttcc agtatatatt
    23821 gttgacaaac attactcaac tgcgctggat cgcgttataa tcaaaatgta acgaagtctt
    23881 atttatgtag taataatagg gcaactctgc ggatacggcg aaaagcttgt taaatatata
    23941 ttcacctact tacttattaa ctatatttga ctaatttgat ataatacttt tgtagacttt
    24001 gggtctacct gattttattc aaattccgcc ttctgagcgg ttttttgatg ttctagtcat
    24061 aacatgaaat atttagctaa atacattaaa ccttatctaa ttgggctcct ggctttactg
    24121 ttttttgttt gggggcaaag ctatgtcaac cttattttac cagattatac tgctaaaata
    24181 attgatcagg gcataatgct aaagaatatt aatattatct ggtcggaagg aacaaaaatg
    24241 cttttgctga ctattttagg aggcttatgc acaattttaa tcggttttat tgccgctaaa
    24301 gtttccgctg gttttgccaa gcgattacgt aaagcggttt ttgataaaat tgagagattt
    24361 tctctaaacg aattcaatat attctcttcc tcatctttaa ttacccgcgc cactaacgat
    24421 gtccagcaaa ttcagaatgt cttatccatg atgttacgta tgtctctgct cgcccctttc
    24481 atgggaatag gcgccattat taaagtgtac caattagctc ccagcctatc ctggattatt
    24541 ttaacggcaa tttgtgtttt agcgctaatg ataacaattt tgtttctaat agctattcct
    24601 aaatttacta ttatccaaaa aatggttgat aagcttggtt tacaaatacg ggaaatgtta
    24661 accggtattc gagtaattag agcctataat aatgaccaga aacaacagct taaatttgat
    24721 aagacaaata ccgaattaat gaatctaaat attcttatta accgattaat atcaatgatg
    24781 caacccgcta tgaccttaat tatggggtta tcagcggtag cagttatctg ggcaggagcc
    24841 tatctcgttg catccggtca tttacaaatt ggcagcatac tagctttaac ccaatatgtc
    24901 tctcaggcga tcatggcttt tcttatgatt tcaattattt ttattataat ccctcgagcg
    24961 accgtatcgg ttcgccgaat taatgaaatt ttatcaatta taccagaaat aaatgacccc
    25021 gctgaaccaa aacacctaac agaaaaaatc catggtttat tagaatttaa aaaagttgat
    25081 tttagttata aaggctcaga acaagccgtc ctctctgata tttcttttat cgcttatcca
    25141 ggacaaacaa ccgccattat cggcggtacc ggcagtggca aaacaaccct cctaaatctc
    25201 atccctcgtc tctatgatgt taccggcggc gctattacta tcgacggcat tgatatccga
    25261 gatttgcccc aacatgaact tcgtgaatac atcggttatg tcccccaaaa agcttcttta
    25321 ttttccggca ctattaaaag caatgttgct tatggccgac caagcgccag tctgactgaa
    25381 atcacggagg cgatcaacac tgcccaggct gccgaattca tcaacgattt agccgatggt
    25441 ctggatagtg ctatcgccca agcaggaaat aatgtttccg gcgggcaaaa acaacgctta
    25501 gccatcgccc gggctttagt caaaaaacca cccatctttt tatttgatga cagtttctcg
    25561 gctcttgatt ttaagaccga tgccgctttg cgccaagctt tacaaaataa tttaaagacc
    25621 tcaacaatta ttattgtcgc ccaaagaatc agcacaatta tgcaagcgga taatattatt
    25681 gttttagata acggaaaaat tgttggtcag ggtaaacacc aagatttatt gacctcctcg
    25741 gctgtttacc gcgaaattgc cgaatcacaa ttatccgcga cggaactaaa gaaaaaataa
    25801 tatggctaaa atagtaaacg gaccttttcg cggcggatct ccaagaaccc tgatgaaggg
    25861 aaaaactgaa aaagcaaaaa attttaagaa aacttttaag atttttataa aatatctaaa
    25921 accttatcgg ttaaaattaa tcttggtttt attatttgct ttagcggcta ccgcctttac
    25981 aattgctagt cccaaaatat taggtcagat gaccgatgtt atcgtccgtg gacttcttaa
    26041 tcataataaa atagatttcg tccaaattac caaaattggc ctcgggctta tttgtttata
    26101 tgctgccagc gctcttttta gttacttaca gagttggata atgtcaaaaa tttctcaaaa
    26161 aattacttat tcttttcgcc ataatattgc cgctaaaatt tccagactac ccttgagtta
    26221 tttcgaccat aatgaaaagg gagatattat cagccgcgtc actaatgacg tggaaacgat
    26281 aagccaaaat ttaaaccaag gtttagtcca agcaattacc gcggcagcga ccgttattgg
    26341 catcttgata atgatgctaa ttatcagttg gcaaatgact ttaattgcct taggaatatt
    26401 gccgttaagt tttattttca tcaaattaat agttaaacat tcccagcacc actatgacac
    26461 ccaacaatcc tctcttggaa aaattaatgg gcatatcgaa gaaatgtttt ctaaccacgc
    26521 tatcgttaaa gcttttaacg gtgaggagcg gtctattaat cattttgatg acatcaacgc
    26581 tgatttatat aatagcggct ggaaatcaca atttttatcc ggcttaatga tgcccctgat
    26641 gcattttatt agtaacctgg gctatgtcgc cgtggctgta gtaagcggct ggttggcttt
    26701 aatgggaaaa gtcagtattg gtggcattca agcttttatc caatacatga accaatttac
    26761 tcagccaatt actcaaacgg ctaatatcgc tagcgtcttt caatcgacag cggcagcggc
    26821 tgaaagaatt tttgaatttt tagaagagga ggaggaaaaa ccggaaacta ataacacccc
    26881 taaaataaaa aatgttcggg gacgcgttga gttcaataaa attaatttcg gttattttcc
    26941 cgaccgacca attattaaag attttaccgc ccaaataaaa ccaaagagta gggtggcgat
    27001 tgttgggcca actggtgccg gcaaaacaac gattgttaat ttgctaatgc gtttttacga
    27061 tatcgacagc ggcgaaatat taattgacgg aactaatatt gaaaagatga aacgctctga
    27121 tgttcggagt ttgtttggga tggttcttca ggacacttgg ctctttaacg acacaattgc
    27181 cgagaatatc gctttcggca aatcaggagc cactcgcgaa gaaataatag aggccgccca
    27241 agaagcccaa atcgatcatc ttattagaac tatgcctaat ggctacgaca caatcataag
    27301 cgattctatc gatagtattt cggtcggcga aaaacaatta ttaacgattg cccgggccat
    27361 tttagccgat gcgccaatgt taattttaga tgaagcaact agctccgttg atacccgcac
    27421 agaaattctt atccaaacgg ccatggaaaa actaactaaa aatcgaacga gctttgttat
    27481 tgctcatcgt ttatcgacta taaaaaatgc cgatttaatt ctagttatgc aaaacggcaa
    27541 tatcgttgaa caaggccaac atcaagaatt actggataaa aaaggttttt attattctct
    27601 ctataatagt caattttaaa aagaggacta atgtctaaag aaaagatata attaaagaaa
    27661 taccaaaaag ccctgtaaat acagggcttc gttagttgtg cgatactaaa ttgtcttggc
    27721 gaagagggtg ggacgcgctg ggaacggttt tattatttaa aaaccgaagt atttcaaaac
    27781 aggcttaatg tttggagcca aatcttcttt atctaagtct tgtatgtcta tccagcgcca
    27841 ctcttttatg tctttacctg gcacgacttc tccgattctt ttagctaaaa agtgaactaa
    27901 aataacactt aaattttctc cttctttttc tttttcggtg taaaaaaaat atgggttatt
    27961 atttagtatt tctatatcca gacccatttc ttccttaact tctctaatgg ccgcttcttt
    28021 taaatttgtt tcaccctcct caacccttcc accacaaaat ttccagaact catcatcacc
    28081 atgtttatta agtaaaactc tattattttc tacaataaca ggacctgagg caattataat
    28141 tttagacata tcttataatt taattattac taaaaccata aacacaaacg aatcaataaa
    28201 cttcttaata aaaatagtcc gtatccagga ctatttagaa aatgcggtga aattatatcc
    28261 tttgctcgaa cctttttcga gcggaaaacg gactgaac
//
LOCUS       DAOQSA010000013        26310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_1147_length_26310_cov_7.710569, whole genome shotgun sequence.
ACCESSION   DAOQSA010000013 DAOQSA010000000
VERSION     DAOQSA010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 26310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26310
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1147_length_26310_cov_7.710569"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PKK65_02170"
     CDS             <1..585
                     /locus_tag="PKK65_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81520.1"
                     /translation="WPNQQLISPTAISSKKEVNMRINNLMLNVQAIDELKLVFRHNGW
                     NKVLKEQAEIKTIEHLIDCDSAPFIPKGWSLEEHRRNGFLKFDPAKISLYLPRKQKKG
                     IEGEELRKILANKPVMNANILDYLLNHPELIPKDWKDKYVFFWGTIYRLPNGVPAVRC
                     LEWDYYKQSWGYVWWWLNRYLRFNYLCPAATFSQ"
     gene            887..1810
                     /locus_tag="PKK65_02175"
     CDS             887..1810
                     /locus_tag="PKK65_02175"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HNU81521.1"
                     /translation="MNKHKITIIGAGMVGSTAAYGLIASDITEEIALIDLNEKLVKSQ
                     VMDLQHSVPFWGYTDVKLGTYDDIRDSKLVVITCGAAQKSGETRLDLLKKNSAIIKDL
                     MPKIFKKNPNIIVLMVTNPVDILTNLAIKMFPKKKRQIFGSGTILDTARFRLILGEKM
                     SVDPKSVHAYIVGEHGDSELPLWSTAMVGNTNIDNFYKLSKSNKKRIFDQAKNAAYAI
                     IKGKQATYYAIAAGIVQLSRAVLFDKKTVFTVSHPLNGEFGIKDVSLSLPVVIGGQGV
                     IRRINMEISAEEKRLLQASATKLKKAEQGLR"
     gene            1988..3916
                     /gene="uvrB"
                     /locus_tag="PKK65_02180"
     CDS             1988..3916
                     /gene="uvrB"
                     /locus_tag="PKK65_02180"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNU81522.1"
                     /translation="MASIFKLKSEFEPAGDQPQAIEKLLKGLEAGFDYQTLLGVTGSG
                     KTFTAAKVIAAYNKPALVIAPNKALAAQLYREYKNFFPENAVHFFVSYYDYYQPEAYL
                     PITDTYIDKEAMINEEMDRLRHGATSALLSRRDVIIVASVSCIYNLGVPLNYLGASLH
                     LEIEQMITRQDLIRQLVSMQFKRVNGEIQRGQFRVRGDVFEIRPMSENVVYRLDLEGQ
                     RVKSLNILDNTTKNILKNLQEIVIFPPKHFISTKPQIEQALKNIEEELQDRLAYFKAN
                     KLFLEAERLERRTRYDIEMLRTLGYCHGIENYSRHLSGKLAGEAPDPLLSYFPLKDGK
                     PDFLTIIDESHITIPQIRGMYNGDRARKDTLVKFGWRLPSALDNRPLKFSEFQKRVGQ
                     VICTTATPGNYEKKHSQQIVEQIIRPTGLVDPEIEIRPVYDKTTNYSQINDIVNEAEK
                     LAAKGERVIINTLTKKQAEDLSAYLNNKKIKANYLHSDIKTFERTDILKSFREGVFDV
                     LIGVNLLREGLDLPEVTLVAILDADREGFLRSETSLMQIMGRAARNVSGKIILYADKM
                     TDSMKRAIAESSKRREKQLAYNKKHGLKPKTVIKKIESMLGLEEEREENKNFKQNKNK
                     LQKYENRKDRSAFLKPRN"
     gene            3972..6500
                     /gene="uvrA"
                     /locus_tag="PKK65_02185"
     CDS             3972..6500
                     /gene="uvrA"
                     /locus_tag="PKK65_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNU81523.1"
                     /translation="MQDKIIIRGARMHNLKNINLEIPHNSLTVITGVSGSGKSSLAFD
                     TIFAEGQRQYVESLSPYMRQFLGQKKPADVDEILGLAPAISIDQKALSHNPRSTVGTL
                     TEIHDYLRLLYSRVGEVFCPHCGLKIEKLTLEEMIDIIISRGKEYKEEYAVIFSPVVR
                     GRKGEYYQLLYDYLALGYSEARVDGEIKNLREPVKLKAKTKHDIEIVIDKVMIKDEGR
                     LFEAVENALQYSQGLVIVKFQKNEFLLSSNWTCPNDNFAFPEVEPRLFSFNSPYGSCP
                     QCFGLGKIGFFGDKICPECKGQRLRPEALSVRLNNKNIAEVSHLSIDKAYDFFWKYYQ
                     KMTKRQLTIADNVMAQILNRLEFLDKVGLNYLSLDREAETLSGGEAQRIRLSSQIGSQ
                     LSHTLYVLDEPTIGLHERDTDRLIKTLETLRDKNNTVIIVEHDARTILASDYLLDLGP
                     LAGVHGGQIEAMGETKKLLAAEEKKPGSQSMTLQYLSGHQEISLPEKRRSQNHGSLKI
                     IGAKINNLKNIKVEIPLGKLVGISGVSGSGKSSLLYDALYKNLSYIKNNPGKKNNLKG
                     VTEIKGAEYINKIVVIDQSPIGRTPRSNPATYTGIFTPIRDFFAELPEAKERGYSISR
                     FSFNRPGGRCEACEGAGSNLIEMHFLPPVLVECEVCRGKRFNRETLQVKYKGKNIANV
                     LEMTIDEAIELFEGNYYITDKLKVLQSVGLGYLKLGQSATTLSGGEAQRVKIGKELTH
                     TLGKRSLYLLDEPTTGLHYYDIAMLLKVLNQLVEKGNSVLVIEHNLHMLKSMDYIIDL
                     GPEGGDKGGKIVATGTPEEIAANPNSLTGQYLQEMLSKKKKAEH"
     gene            6698..7021
                     /locus_tag="PKK65_02190"
     CDS             6698..7021
                     /locus_tag="PKK65_02190"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNU81524.1"
                     /translation="MKLDKNIKIRPFAEQVYQITKKIKVGQVATYRQIAQAIGKPLAA
                     RAVGQVLHNNPFAPQVPCHRVIKSDGRLGGFARGSKKKLSLLRQEGIIIKNNSLDLNK
                     YQAKL"
     gene            7024..8190
                     /locus_tag="PKK65_02195"
     CDS             7024..8190
                     /locus_tag="PKK65_02195"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNU81525.1"
                     /translation="MAHPLKNLLKDIPKMPGVYFWYGQKGEVLYVGRAVNLKNRLSQY
                     FQKRIDSRISEMVAQAQDLKFIICDSLLEAIILEAANIKKYWPKYNIVDRDDRSFIYI
                     AINLKEEFPKPIIIRGQDLKKFPARDYQFFGPYQSYRLISTALRLLRKVFPYSTCRPH
                     QGKACFDYQIGFCPGACIDAISPQEYKKNIKKLILILKGERQRLLLKLEKDNPEQAKA
                     LRHLQEVSLLNKEDDLQSPRLKRIEGYDISHFQGKESYASMVVFINGEKQANEYRLFK
                     IKEAPAGDDERALLETLKRRYQHKEWPPAEMIMIDGGSPQISFLSRELSKLNIILPLV
                     GISKFGGDKLVFPKGVKQNFKDLAESLKPVLLKVREESHRFANYARKRKTNTIN"
     misc_feature    8218..8338
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            8375..8905
                     /gene="rplJ"
                     /locus_tag="PKK65_02200"
     CDS             8375..8905
                     /gene="rplJ"
                     /locus_tag="PKK65_02200"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNU81526.1"
                     /translation="MPKSKVQKQEILRSLEKKIGDAKSIVFAGFNALGVKDNESLRDQ
                     LRQENGEYYVAKKTLLGLALKNQGVDGLDTKSLGGKIAAVFSYGDEVAAAKVLDKFRQ
                     DKEKSDKLNFIAGILEGKLLTKEEIEALAKLPSKQELYAKLVGSLNAPVSGFVNALAG
                     NLRNLVYVLKAIEEKK"
     gene            8961..9389
                     /gene="rplL"
                     /locus_tag="PKK65_02205"
     CDS             8961..9389
                     /gene="rplL"
                     /locus_tag="PKK65_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNU81527.1"
                     /translation="MSEEVKNDAAEEVVVPAKFQSLVGEIEKMSVLDLAELVKILEKK
                     FGVSAAAPAMMMAPTAGGANASAAAAEEKDSFDVELTEGGANKINVIKVVREVTEMGL
                     KEAKDLVDGAPKILKEGLKKEEAEALKKKLEEAGAKVTLK"
     gene            complement(9465..11705)
                     /locus_tag="PKK65_02210"
     CDS             complement(9465..11705)
                     /locus_tag="PKK65_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81528.1"
                     /translation="MYYKISSFTLNTGKHQNGSREVFIFQPDAIKENLAGKLFLLAEI
                     DGKKNDVKKVIDFIIERLNDFYYNDEKIFLQDKIEGLSLDNIFEAALAKLNKSLLEFI
                     SLEKIILRAEDTNLVLGLIFDNKLLFSNFGRNKAFLIYKRQDDYELVNVETSAVEADN
                     QPESTGPVSPKFFSSVISGEIPASSYFLFCNEALPEYLSNHDLINIITKLPPMVAAEQ
                     IKNNLTRLNSYVPFLGIIIKNTFGLSLAELKEDMGGDTVQSAHNSISHLNYTEKKTEQ
                     MLASAGLINWQNINKIWKKVKGGSFKPGPSLSIKDSGKNIKKTPSLKQSKTLATPNLI
                     KEKIAFGRSRSRLTGFLKTLSATIINLFNPSFWRKFAKNVKIWLNSLHPRNKQMLVLL
                     LLAFLILSVSLIISSINNRRQADIEYFHNSINDLETKKSMIDSYLSYDNQEGAKTLIG
                     ESLAAIDVLPVKGDEQIAKRDQLRAQLEAQKAQLQKLSTVESPELLTDLRHYNPSAEA
                     RNLILLDEKLYVADSAAKAVYTFDLKDKNTASFLLNGDFNALDKPVSEAGSIYYLSGN
                     RLISLDTVSGKVTTLNLDGVGADNKINAFQFYRSNLYLLMADQNQVYKLSKNATGFGS
                     KVARLNGEAYLGDVIDMAVTGDIIFLKSNGQIQDYYNGEPKDFSLASIDPSLNSASLL
                     KVFNDQLFIMDKSSQRILIFDKQGVLVKQFRLPSLNNLKDFSVSADGKTIYVLNDNSI
                     YIVPTE"
     gene            11839..12462
                     /locus_tag="PKK65_02215"
     CDS             11839..12462
                     /locus_tag="PKK65_02215"
                     /inference="COORDINATES: protein motif:HMM:NF013209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HNU81529.1"
                     /translation="MLAQIFGSNARVKIMKTFLSKPEQKYYTRQLARDLNLQVNSVRR
                     ELKNLEEIGLIKQEEAKILSEVKETKIKTGAKNLKNEKKYFTIDRNFLLFPELKTLFA
                     KANLLSCQDFLKNTAEFGEIKQLWLSGIFSGDGSAPIDMLLVGKIKKDKFIKRLQNLE
                     ADLNKEINYTLMDEAEYEYRLEIHDIFLNRIRQSRHLKIINSEKENN"
     gene            12471..14411
                     /locus_tag="PKK65_02220"
     CDS             12471..14411
                     /locus_tag="PKK65_02220"
                     /inference="COORDINATES: protein motif:HMM:NF024594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HNU81530.1"
                     /translation="MKVKANLKYFALLSLRLLLWALAIFFLSLIISLISSWSYWRDFG
                     QNAIAGKEQLSGAIVSIRAKDWDGAQLKLQSAQDYFKNGQKNLEKLRQSWFPAKLKLG
                     SRPIDDLGYLNNSALIISGSALQVNSLLKNISADAFANERNFQSLSQEKKAAFMQSLI
                     ALEPELNGLKANLNLALFNLDKIQTVGILWPLRGQLAELRNQIADGENILSNSLPIIR
                     LLPAFSGYPQPVHYLIMLQNNDELRPTGGFLGSYVRADITNFGEFQKLEANDIYHLDM
                     PAIGKTAFVAPEPISKYLKVDNWYLRDANWSPDWPQSARQIQIMFQAESAASGKAEPN
                     LDGIFAITPDFIANLLRLTGPITVRGESYAPENMQALLQYNVEVAYKEEEISSWDRKD
                     IINELIQALKEKIIALPLEKYPELISCLNDSLKRGDILLYFNNQNLQTLAQNLGGGGE
                     IKQVSGDYLMVVDANLAAFKSDAVMQKNIAYQIERKQERLQANLKLNYYHNGGFDWRT
                     THYRSYTRVLAPLGSELIRLSGLEATAAEDIISYDDDSLKKHVFGFFWKIEPGKNKVI
                     NLEYYLPAKLSDNLSTNNVYTLYIQRQPGSRIANLSFSFNPNKNIIQISPQSLEGEIK
                     SQKAYFTLDLNNGSQAIQIKTD"
     gene            14637..15698
                     /locus_tag="PKK65_02225"
     CDS             14637..15698
                     /locus_tag="PKK65_02225"
                     /inference="COORDINATES: protein motif:HMM:NF026235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HNU81531.1"
                     /translation="MKKLILITVFFVFFTIASWAQKLANLWYLPTMTTYQAENVSQYD
                     LLVIDPEILVNHSSTLDEMMANNPKLEIFLYINPTEIFEPMWPDKPWSIKLYQELTTK
                     KSWWLYHPNGEKLGIWAGMKTLDMRANCPEIDGQRYWQWILDQYLKLLTDKRISGCLI
                     DNAWGDDRAGIQWLANYNSQKGLDLNGDKKADTNWQEINLAWAKGFHSFFGELRKVKD
                     NLCIIANPGNLSYREVDGKQFENFPYPHHHLGGPDWEVNMMIAKKYKIAIINPDEKDF
                     LLGVISSLMLDNAFLAVGQNQLYHDYYNLNLGKALGKTKEISKGIWFRQFEKGSIFID
                     TNKGNKAWVEYKDGTKREK"
     gene            complement(15774..17126)
                     /locus_tag="PKK65_02230"
     CDS             complement(15774..17126)
                     /locus_tag="PKK65_02230"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNU81532.1"
                     /translation="MPSLLNNKKSVIALTFIAVFTALLLLFSNLSLPNVLLSSLLLLL
                     LALSFIDFRLAFLSILFLRPILDLSVHNIIFSFGDLQINILSLLGILMIILAIIFIFL
                     GKFDWHNLKQLHSFYAWVIFLFLGIISTFNSFYFSESLKEILRFFSIFAAFFLGASLL
                     PSAKNLTWLIKVIIFSALPPSLIALFQFINKTGLPENGIYRVFGSMTHPNMLAFYLLL
                     AITLAVFLLLNLKRTKLEVYFYSLLSLFYIFILFLTYTRGAYLALIIIFVIVGVSKFK
                     KFLLVAFLAMLFVYALVPPIQTRFNSIFQSDPYGSVSWRFNLWRDGLEYFQERPWTGY
                     GIGTAEKIISVKRDFRLGPPDPHNDYLRVALDGGYPLLASYILLLLSFFGAIISSYWH
                     ESRPRLKNFFLFFLAFGVAVFTMSSGDNILNDTALQWQMWALAGSALACARLKPDLSA
                     "
     gene            17218..18435
                     /locus_tag="PKK65_02235"
     CDS             17218..18435
                     /locus_tag="PKK65_02235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNU81533.1"
                     /translation="MKILQVNKFYYPHRGADKYFLFLEKLLKSHGHEVRVFAMSSAKN
                     LESPDAPYFAEEIDLHNLTWKNKIKASRAIIYNQEAKRKFKKLLADFQPDLIHCHNIY
                     HQLSPSILDAARQAKIPVVMHLHDYKLICPNYRLYTEGSACQRCCQQRYWECVKHRCL
                     ENNFSKSLLAFIEMTVHHRFLKIYKKGVTLFIAPSEFMKKICIDFGWQDDKFIVLENI
                     SPINSTKINSEIKEYFLYFGALEQEKGIDLLIRAGAESKRPIKLAGTGRAEHELRNLA
                     LKLKADVEFLGQLQGDKLHQVIAESLAVVIPSRWPENMPLAAIEAMGLGKTVIAANIG
                     GLSELIKTGDNGYLFPPEDWQQLAEYLKQLQAPEALEIGKKAAASRKDKTEDWHYQKL
                     ITIYQTVINKKEL"
     gene            complement(18471..19763)
                     /locus_tag="PKK65_02240"
     CDS             complement(18471..19763)
                     /locus_tag="PKK65_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81534.1"
                     /translation="MKKIFLSALIFGLLFVGWSVRDGKTKDQSYYAGDAVLYKGQLIV
                     ATANTGKLEIFSSSGNKVKHLMSVSLSPNPGKEDVFNDVKLEVQGQSLKAYAVAGYAL
                     YLYDISDLQTASLENKVKNTYWEWYHRVDRFGHNIATVSGRGVRIWNNDLQIIDGFDF
                     ESELPYSLRSSGDARFLFALNNSALNIYDRESRHVIKTISLDYRDFKKNGRKSYYDRI
                     SGNIFVADDYYVKKFDFNGKLLASFRHYGDASYDVESSFDNNFLYFSNGLNVYKLKKS
                     DLSLVKEVQATTLGAPQGWAMGLKLVNTSDGDRLVVFNNSGLLILDANLNVVSKTDKI
                     SQDDGQLYPLENLYLMLGNRSAQSGASVSVQGGGFWPNENLSISLIGSKTNAQADRFG
                     RFKANVIVPSVPNDGRYDIKVDGLNSQLTYSISLEVKK"
     gene            20040..20810
                     /locus_tag="PKK65_02245"
     CDS             20040..20810
                     /locus_tag="PKK65_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81535.1"
                     /translation="MELEVFWKNIKKRRHTVVGIILISFILVGTATFLQPLKYSVKSR
                     LLISQNISGADPYTVSKSNQYLSNLFSQVVYSSSFFNLTTSAGFNIDTSYFGNDYKTQ
                     MKVWKKTIEARSVGDTGIMEIYIYHPNTYQAQQIALAVNQTLISKSFNYQQGGDQVII
                     SVIDQPLTSSFPIKPNLILNFAAALILGIAATSLWIYFFPIKEHKHQPKKQNNLVYQE
                     LPTENYSTYPAAPSNLPTEDTKADNYYEPRGNINNILK"
     gene            20913..21755
                     /locus_tag="PKK65_02250"
     CDS             20913..21755
                     /locus_tag="PKK65_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81536.1"
                     /translation="MQKIFFIMMATLLAIAGKEASAQHPLGEGQLLIGNGYFAPANKS
                     GDGTYRYLETRWLPIKNQTDNAKFGFYLSGIEVGSKISGFKHHSTEIGLGLAANFSLQ
                     PGYQTDRYAWLNLAYKVINSTGSLTKTDGRYENRQEDQMLFLGGGLLFRNIFLEGPFA
                     QQKIMLESQFSIKSEENSRWNGDTLAGLPWSRERVKLQAENGITPLYLGWSRNVYLMP
                     SILAAYTYEKGSQTSFYTIGLSLTLAKGQYGYEILTLSYEPKFSAKGPRMDVFQVNLN
                     IINL"
     gene            21838..22545
                     /locus_tag="PKK65_02255"
     CDS             21838..22545
                     /locus_tag="PKK65_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81537.1"
                     /translation="MVLLTFLRGGKNSTPVAAVADSTTVTTPAAPCPPFTVGINISGD
                     SLVRMYQEIARLQAALKACEKGKKPVTQARKSSSTSAASNQTRTSSTSAASASQSTSP
                     MIATNQYVGDVVGDAGCSFDENSKLFFYVKTSLLNSIKNRTQTTSNLNAPSGPKGEEV
                     GEYTYYKTPILILNDMLDQEWAWTVYVGNNAQYGYDMWLWHELIKLNADLKNDPQIQS
                     NGLGGYQFISKINYHSR"
     gene            complement(22658..23821)
                     /locus_tag="PKK65_02260"
     CDS             complement(22658..23821)
                     /locus_tag="PKK65_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81538.1"
                     /translation="MKRFTLFTFLFALALIFTGCMKEYDDASPISAPKSAPYLKVAGA
                     NMSGDTAYAAPNVFLKFWLDSLPSAVENYTFSWNLDGGDYSTATSPEKKYAAGIYDIS
                     VVITPINGGLSITRSITLVVSNDLTWETTLILLSATPVAGNNYDYAIAMRTTAVYNYA
                     NIAADPWDRGDFTGWDLNYLTETTTINGILYIIDHVILPANEIGVQRFTYGKGNVYAY
                     APESRYWVVIAPGEGVFEVYLTNGQMSPNPVGAGLIPGDNGDLIGGVYAPTVRTEIKY
                     SNLPLSDSLLVYINYQQYANGPQPFITRMLSNDNWQPMALIIMSGAYTGWGYQTFAIK
                     DLEGHLYFRFGPSLTSPNVYGEMANSKFFLPGDNMLGLQITSLKSGAYEIRRM"
     gene            complement(23923..25032)
                     /locus_tag="PKK65_02265"
     CDS             complement(23923..25032)
                     /locus_tag="PKK65_02265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNU81539.1"
                     /translation="MKIAFIGQKGIPAQTGGVEKQVEELLIHLAARGHDVYAYARRGY
                     APEISEYKGVKIIPLPFIKGKNFEAISNTFLAVLHSAFRRFDIIHFQSIGPASLIWLA
                     KLLNPRTPIVFTFHCQDYYHKKWGLFARWYLRLGEKIGCRLADQTLVTSQELTIYALA
                     RYDKGAVYVPSGINPPTLPEVREIRRWGLEKDNYIFIASRLIRHKGIHYVIKAFKNLK
                     TDKKLVIAGEGAYTDDYVKELKELAGDDKRIIFVGNQGGALLSELYANAYMFIQSSEY
                     EGLSMGLLEAMSYGLPCLASDIPANLEAMNGLGLSFRNKDVSDLSHKLQYALDNQEIV
                     KAQGQALRERTRQEYNWEKIVDQTIAIYNRLLAKK"
     gene            complement(25226..26191)
                     /locus_tag="PKK65_02270"
     CDS             complement(25226..26191)
                     /locus_tag="PKK65_02270"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNU81540.1"
                     /translation="MGYYKKISQKIECLLIDKPGTEQSLSWINIIDAGRKEIDYLRKE
                     YNFNLEYLRASVATVFSQRPMVAEDNGYLFLILHFPTFFKGKIIAGEIEFFIGHGYLV
                     SLHNDNLPALNELFNNAKKDQNSLLSYNTESSATLLYEILDRLLNSCYSLIDQNSLAI
                     EEVEDLIFEQQQKDAVAKILNLRRNIINIRKIMQNHKNILQKLMSMRSSLVPPDSLKK
                     QYGHLVEHSKRIWEMLDNQKEMVEVLNDTNESLLNDRMNNIMKTLTLFSVIVLPLTLL
                     AAIFGMNAKYMPFVDQRYGFWIILLIMGLGSWLMFYYFKKKDWLN"
BASE COUNT         8916 a         5182 c         5020 g         7192 t
ORIGIN      
        1 tggccaaatc aacaactgat tagcccaaca gcgatatcca gtaaaaagga ggtaaacatg
       61 agaatcaata atttaatgct caatgttcaa gccattgatg aattaaaatt agttttccgc
      121 cacaatggtt ggaataaagt tttaaaagaa caagcagaaa tcaaaactat cgaacaccta
      181 attgattgtg attctgcccc ttttatcccc aagggctggt cgcttgaaga acaccggagg
      241 aatggatttt taaaattcga ccctgccaaa atttccctct atctgcctag aaagcagaaa
      301 aaaggaatag agggggaaga attaaggaaa atattggcga ataaaccggt tatgaacgcc
      361 aatattttag actatctgtt gaatcatccc gagttgatcc caaaagactg gaaagataaa
      421 tatgttttct tctggggtac gatctatcga cttcccaacg gcgtccctgc cgttcgttgt
      481 cttgagtggg attattacaa gcagagctgg ggctatgtat ggtggtggct taatcgttat
      541 ttaaggttca attacctttg tcctgctgcc accttttcgc agtagtgagg tttttattat
      601 ttgaccttgg tttataaaca taacgtttat ataaccaagg tcttttttta taaacaataa
      661 ttttaattaa ctattatata aatgtaaaaa atggtcatta ttataataat gtgttaatat
      721 tattaataaa tacgaatgat taattatatt ttatgattag aaaaatatat tattagccat
      781 atataaaaat ttgactccgt attaacgggc tctctaataa ataatccgtt gaatattggc
      841 atttgggaaa attaaaattt taaaataaaa aaaacaagat aattctatga acaaacacaa
      901 aattacgatt atcggtgccg gcatggttgg tagcaccgcc gcctatggtt tgattgcttc
      961 cgacattact gaagaaatcg ccttaattga ccttaacgaa aaattagtaa aatcacaggt
     1021 gatggattta cagcattccg tccctttttg gggatatact gatgttaagc ttggaactta
     1081 tgacgatatt agagatagta aactagtagt tataacttgc ggcgcggctc agaaatcggg
     1141 tgaaaccagg ctagatttgt taaagaaaaa ttccgccatt atcaaagatt taatgcctaa
     1201 aatatttaaa aagaatccca acataatcgt cttaatggta actaatccgg tggacatact
     1261 caccaacctc gccattaaaa tgtttcccaa aaagaaaagg caaattttcg gttctgggac
     1321 cattctcgat actgctagat ttcgtcttat tttgggcgaa aaaatgtcgg ttgaccccaa
     1381 aagtgttcat gcttatattg ttggtgaaca tggcgatagc gaactacctc tttggagcac
     1441 agctatggtc gggaatacaa atattgataa tttttataaa ttgtcaaaat caaataaaaa
     1501 aagaatattt gatcaggcaa aaaacgccgc ctatgccatt attaaaggca aacaagcaac
     1561 atattacgct attgccgccg gtattgttca attaagccgc gccgttctat ttgataaaaa
     1621 aacagttttt accgtttccc atcccttaaa cggagagttt ggcattaagg acgtttcttt
     1681 aagcttgccc gtagttattg gcgggcaggg ggttatacgc cgaattaaca tggaaatttc
     1741 ggccgaagaa aaaagactgt tgcaagcttc agcaaccaaa ttaaaaaagg ccgagcaagg
     1801 attgagataa taattgtttt tcatcattat aaaaaaatat accgacatct gcttaaagcc
     1861 gctccttaat gccaataccc ggcccattcc gcgcaaaaat tgccatacag ccctaaataa
     1921 cagggctggt ttttttattg attttatgct acattaagca aagattaatc aataattcag
     1981 attaataatg gccagcattt ttaagctaaa atcagaattt gagcctgccg gcgatcaacc
     2041 gcaagccata gaaaaactcc tcaaaggatt ggaggctggt tttgattatc aaaccctact
     2101 gggtgtaact ggctccggta aaacttttac cgcggctaaa gtcattgccg cctacaataa
     2161 accggcttta gtgattgctc ctaacaaagc tctggccgct caattatacc gagaatataa
     2221 aaacttcttt ccggaaaatg ctgttcattt ttttgtgtca tattatgatt attatcagcc
     2281 ggaagcatat ttacctatca ctgataccta tatcgacaaa gaagcgatga ttaatgaaga
     2341 aatggacaga ttgcgccacg gagctacttc ggctctttta agccggcgtg acgtcattat
     2401 tgtcgcctcc gtttcctgca tatataatct cggcgtacct cttaattact tgggcgcttc
     2461 tttacattta gaaatagagc aaatgattac ccgccaagat ttaattaggc agctggtgtc
     2521 catgcaattt aaaagagtaa atggtgaaat tcagcgtgga caattccgag tacggggaga
     2581 tgtttttgaa atcaggccaa tgtcggaaaa cgtggtctat cgtttagact tagaagggca
     2641 gagggtaaaa agcttaaata tccttgataa cactaccaaa aatattctga aaaatttgca
     2701 agaaattgtg atttttccgc ccaagcactt tatttctaca aaaccgcaaa ttgaacaagc
     2761 gcttaaaaac attgaagaag aattacaaga taggctggcc tactttaaag ctaataaatt
     2821 atttctggaa gcagaaagat tggaacggcg cactcgctat gatatcgaga tgctgcgcac
     2881 tttaggttat tgccacggca ttgaaaatta ttcccgccat ttatcgggca aacttgccgg
     2941 agaggccccg gatccacttt taagttattt tcctctcaaa gacggcaaac cagacttttt
     3001 aaccattatc gacgaatccc atattaccat tcctcaaatt aggggtatgt ataatggtga
     3061 tcgggcacgc aaagatacct tagttaaatt tggctggcgt ttgcccagtg ccttggataa
     3121 ccgccccctt aaattttccg aatttcaaaa aagagtaggt caagttattt gcaccaccgc
     3181 cactcctgga aattatgaaa aaaaacattc tcagcagatt gtcgaacaaa ttattcgccc
     3241 cacgggctta gttgatccgg agattgaaat cagaccagtt tatgacaaaa caactaatta
     3301 tagccagatt aatgacattg tgaacgaggc agagaaattg gctgctaaag gagagcgcgt
     3361 gatcatcaac accttaacca aaaaacaagc agaagatctc agtgcttatt taaataacaa
     3421 aaaaatcaaa gccaattatc tccactcgga cattaagacc ttcgaacgca ctgatatctt
     3481 aaaatctttc cgtgaaggtg tgtttgacgt cctcatcggc gttaatctct tgcgcgaagg
     3541 cttggatttg ccggaagtga ctttagtggc gattttagac gccgaccgcg agggcttttt
     3601 gcgcagcgaa acctcgctga tgcagataat gggccgcgct gcccgcaatg tttccggaaa
     3661 aattattctt tatgccgata agatgaccga ttcaatgaaa agagccatag cagaatcaag
     3721 caaaagacgc gaaaaacagt tagcgtataa taaaaaacac ggccttaaac cgaaaacggt
     3781 tataaaaaaa attgaaagca tgctgggatt agaagaggaa agggaagaaa ataaaaattt
     3841 caaacaaaat aaaaataagc tgcaaaaata tgaaaaccga aaagataggt ccgctttttt
     3901 gaagccacgg aattaatgat taagcattat ttcaccagaa taatttatgg ctacttatag
     3961 acaacaaaaa tatgcaggat aaaataatca tccgcggcgc taggatgcac aatttaaaaa
     4021 atatcaacct ggaaataccg cataacagct tgacggtaat caccggcgtt tccggtagtg
     4081 gcaaatcgtc gctggctttt gatactattt ttgcggaagg gcagagacaa tatgtggagt
     4141 cgctctcgcc ctatatgcgc caatttttag ggcagaaaaa accagcggat gtcgatgaaa
     4201 ttttaggttt ggctccagcg atatccatag atcaaaaagc tctatcccat aacccccgtt
     4261 ccaccgtcgg tactttgaca gaaattcatg attatctgcg ccttctttat tcgcgggtgg
     4321 gcgaagtctt ctgtcctcac tgcggcctta aaatcgaaaa actaacactg gaagaaatga
     4381 ttgatatcat tatctctcga ggcaaagaat ataaagaaga atatgccgtt atctttagtc
     4441 cggtagtacg cggccgtaaa ggcgaatatt atcagctttt atacgactat ttagccttag
     4501 ggtattcgga agccagggtt gacggagaaa taaaaaattt aagagaacca gttaaactaa
     4561 aagccaaaac taagcacgat attgaaatag tgattgataa agtaatgatt aaagacgaag
     4621 gccgtttatt tgaagccgtg gaaaatgcct tgcagtacag tcaaggcttg gtcattgtta
     4681 aatttcaaaa aaatgaattt ctgttatcat cgaactggac ttgtccgaac gacaacttcg
     4741 cttttccgga agtagaaccg cgcctttttt ctttcaactc tccctatggc tcttgtccgc
     4801 aatgtttcgg cttgggaaaa atcggttttt tcggagataa aatctgcccc gaatgtaaag
     4861 gccaaagact gagaccagaa gctttgtcgg tgcgcttaaa caacaaaaat atagctgaag
     4921 tcagccattt aagcattgat aaagcctatg acttcttttg gaaatattac caaaagatga
     4981 ctaagcgcca gctgacaatt gccgacaatg tgatggctca aattctcaac cgtttagaat
     5041 ttctagataa agtcggcttg aactacctgt ctttagacag ggaagcggaa actctttccg
     5101 ggggcgaagc ccagcgcatt cgcttgtctt cccaaatcgg ttcccagctt tcccacactc
     5161 tttatgtttt agacgaaccg actattggcc tgcacgaacg cgacaccgac agactcatca
     5221 aaacactgga gaccctaaga gataaaaata ataccgtcat catcgttgaa catgacgcgc
     5281 gcacgatttt agcttcagac tatctgcttg atttaggacc gctggctggc gttcacggcg
     5341 gccaaatcga ggccatgggc gagactaaga aattattagc ggctgaagaa aaaaaaccgg
     5401 gcagccaatc gatgactctg caatacctga gcggtcatca agaaatttcc ttaccggaaa
     5461 aaagacgcag tcagaatcat ggcagcctaa aaatcatcgg cgctaaaatt aataatctaa
     5521 aaaatataaa agtagagata ccactgggaa aactagttgg tattagtggc gtttccggct
     5581 ccggcaaatc atctttactg tatgatgctt tatataaaaa tctatcttat ataaaaaata
     5641 accccggtaa gaaaaataat cttaaaggag tcacggaaat aaagggggcg gaatatatta
     5701 ataaaattgt agttatcgat caatcgccta tcggccgtac gccccgctct aatcccgcca
     5761 cctataccgg catatttacg ccaatcaggg atttcttcgc tgaattgccg gaagctaaag
     5821 agagaggcta ttctatctcc cgcttctcct ttaatcgacc cggtggtcgc tgcgaggcct
     5881 gcgaaggcgc tggctccaat ctgattgaga tgcatttctt gccgccggtg ctggtagaat
     5941 gcgaggtatg cagaggaaaa cgttttaacc gggaaaccct gcaagtgaaa tataagggca
     6001 aaaacattgc caacgtctta gaaatgacga ttgatgaagc gatagagctg tttgaaggaa
     6061 attattatat taccgacaag cttaaagttc tacaatcggt cggcttgggc tatctcaaac
     6121 tcggacaatc ggccaccact ttatccggag gcgaagcgca gagagtaaaa attggcaaag
     6181 aactgactca cactttaggg aaacgcagcc tctatttatt agacgaaccg actaccggtc
     6241 tccattatta tgatatcgct atgctgctta aagttttaaa ccaattggtg gagaagggaa
     6301 attccgtatt agttatagaa cataatctgc atatgctcaa atccatggac tacatcattg
     6361 atttggggcc ggaaggcggc gacaagggcg gtaagatagt agccaccggc acgccggaag
     6421 aaatcgccgc taatcctaat agcttaaccg ggcaatattt acaagaaatg ctttcaaaaa
     6481 agaaaaaggc cgaacattga atgcccggcc agtcaacctc cgttttaagt ggttagcttt
     6541 tagagaaaga caaaaaagaa gcataaaaaa gataaatata ataatatcta cgacgagagt
     6601 gaatgagttt gggtcatcag gccaaaatat atcataaaat gttaggcctg ccattactaa
     6661 taaaacgcaa ctcactaaat atcccaataa aatcaaaatg aaattagata aaaatattaa
     6721 aattagacct tttgcggaac aagtctatca aataactaaa aaaattaagg tgggtcaagt
     6781 ggcaacttac agacaaatcg ctcaagcaat aggcaaacct ttagccgccc gcgctgttgg
     6841 ccaagttctg cataacaacc cctttgcgcc gcaggtacct tgtcatcggg tgattaaaag
     6901 cgacgggcgt cttggcggct ttgctagggg aagcaaaaag aaactaagcc ttttgcgtca
     6961 agaaggaata ataattaaaa ataatagtct ggacttaaat aaatatcaag caaagctgta
     7021 aatatggctc atcctctaaa aaatttatta aaagatatcc ctaaaatgcc cggcgtctat
     7081 ttttggtacg gacaaaaagg agaagtctta tatgttggcc gggcggtcaa tttaaaaaac
     7141 cgcctaagtc aatattttca aaaaaggata gactctcgca tctctgagat ggtggcgcag
     7201 gcgcaagatt taaaatttat catctgtgac agcttgctgg aagcgattat tttagaggcg
     7261 gctaatataa aaaaatactg gcccaaatat aatattgtcg accgagatga ccgttccttt
     7321 atttacatcg ccattaatct aaaagaagaa tttcccaagc caatcattat ccgcggacaa
     7381 gatttaaaaa aatttcccgc ccgcgactac cagttttttg gaccatacca atcatatcgt
     7441 ctcattagca ccgctttgcg gctattgcgt aaggttttcc cctatagcac ctgccggccg
     7501 catcagggaa aggcttgttt tgattatcaa atcggctttt gcccgggagc ctgtattgac
     7561 gctatcagcc cgcaagaata taaaaaaaat attaaaaaac taatcttgat tcttaagggc
     7621 gaacgccagc gcctcttatt aaaattagag aaagataatc cggaacaggc taaagccctg
     7681 cgccacctcc aagaagtttc tttattaaac aaagaagatg atttgcagtc accgcggctt
     7741 aaacggatag aaggctatga tatttcccat ttccaaggca aagaaagcta tgcttccatg
     7801 gtggttttta taaacggcga aaaacaagca aatgaatatc gtttgtttaa aattaaagaa
     7861 gcgccagccg gagacgatga acgcgcgctc ctagaaacgc tgaaacgtcg ctatcagcac
     7921 aaagaatggc cgccagcgga gatgataatg attgatggcg gcagtccgca aatcagcttc
     7981 ttaagcagag aattaagcaa acttaatatt attctacctt tagtcggcat atctaagttt
     8041 ggcggcgata agctagtttt tcctaagggc gtcaagcaaa attttaaaga tttggccgaa
     8101 tcgcttaaac cagttttatt gaaagtaagg gaagaatcgc accgctttgc caattatgct
     8161 cgaaaaagaa aaacaaatac aattaattga caatttttta taatctgtta atatataaat
     8221 acaaattaag caaaaccgca gacagcaagc aaacaaataa gtcttgccga ggtaaatgtt
     8281 agctattttt agcttattta tttcatctgc ggtcaagccg cagtttttta tttaattgct
     8341 tacaaaccag cgtctaaaaa cgcttttaaa aaatatgcca aaaagcaaag tccaaaagca
     8401 ggaaatcctg cggagcctgg agaagaagat tggagacgct aaatcaatcg tctttgccgg
     8461 ctttaatgct ttgggggtaa aagacaatga atctctgcgc gaccagttac gccaagaaaa
     8521 cggcgaatac tatgtagcca agaagacttt attgggtcta gctctcaaaa atcaaggtgt
     8581 ggacggcttg gatactaaga gcttgggcgg taaaatcgcc gctgtcttct cctacggaga
     8641 cgaagtggcc gccgccaagg ttctcgacaa attccgccaa gacaaagaaa agagcgacaa
     8701 actcaacttt atcgccggca tcttggaagg taaattactg accaaggaag aaatcgaggc
     8761 cttagcgaaa ttaccgagca agcaagaatt gtatgccaaa ctggtgggct cccttaatgc
     8821 ccctgtctcc ggttttgtta acgctttagc cggaaactta cgcaaccttg tatacgtcct
     8881 taaagcgatt gaggaaaaaa aataataata aataggggtc aggaaaagga aaagcttccg
     8941 ttcccgccta atataacatt atgtccgaag aagtaaaaaa tgacgccgct gaagaagtgg
     9001 tggtaccggc taagttccaa agcttagttg gcgaaattga aaagatgagt gttttagatt
     9061 tagctgagct cgtcaaaatt ttggaaaaga aatttggtgt ttctgcggcc gcgccagcaa
     9121 tgatgatggc tccgaccgcc ggtggcgcta acgctagcgc ggctgctgct gaagaaaaag
     9181 acagttttga tgtggaatta actgaaggcg gcgctaataa aatcaacgtc atcaaagttg
     9241 tccgcgaagt cacagaaatg ggcttaaagg aggccaaaga cttggttgat ggcgctccga
     9301 aaatcttaaa ggaaggctta aagaaagaag aagccgaagc tttaaagaag aaattggaag
     9361 aagctggcgc taaagtaact ttgaaataag cgcaaattca gaataaaaaa taccccacgt
     9421 aaaatctttt ggtcacgtgg ggtattttta tattcaaaat taatttattc tgtgggtact
     9481 atataaatag aattatcatt taaaacataa atggtttttc catcagcgct aacactaaaa
     9541 tctttaagat tatttaaaga gggaagacga aattgcttga ctaaaacgcc ctgtttatca
     9601 aaaattaaaa tgcgctggct agatttgtcc attataaaca gctgatcgtt aaacacttta
     9661 agcagggaag cgctgtttaa agaagggtca atagaggcta aagaaaaatc cttaggctcg
     9721 ccattataat aatcttgaat ttgtccgtta ctcttcaaaa aaataatatc accagtaacc
     9781 gccatatcaa tcacgtcgcc aagataagct tcgccattca aacgcgctac cttggaacca
     9841 aagccagtgg cgttcttgga taatttgtaa acctgattct gatcagccat tagtaaatat
     9901 aaattagagc gataaaattg gaaagcgtta attttattat cagcacccac gccatcgaga
     9961 tttaaagtcg taactttgcc gctgactgta tccaaggaaa ttaaacgatt gccactaaga
    10021 tagtaaattg aacccgcctc gctgacaggc ttatccaaag cattaaaatc accgttaagc
    10081 aagaaagagg cggtattttt atcttttaaa tcaaaggtat agacggcttt agcggcgcta
    10141 tcggcgacgt ataatttttc gtccaacaga ataagattcc tggcctctgc ggaaggattg
    10201 taatgacgca aatcagtaag taattctgga gactcaacgg tgcttaattt ttgtaattgc
    10261 gctttctgag cttcaagttg agcccgcaac tgatcacgct tggcaatctg ttcgtcacct
    10321 tttaccggca aaacatcaat agccgccaaa ctttcaccaa tcaaagtttt ggcaccttct
    10381 tgattgtcgt acgacagata agaatcaatc atgctttttt ttgtctctaa atcgttaatt
    10441 gaattatgaa aatactcaat atcagcttga cgacgattat tgatactaga aattatcaag
    10501 ctaacactta aaattaaaaa agccaataac aaaaggacaa gcatctgttt attgcgagga
    10561 tgaagagagt taagccaaat tttaacgttt ttagcaaatt tacgccaaaa tgaaggatta
    10621 aacaaattaa taatagtggc gctgagtgtc tttaaaaagc cagtcagacg agaacgactg
    10681 cgaccaaaag caattttttc cttaatcaag ttgggggtgg ctagagtttt tgactgcttt
    10741 aaagatggtg tttttttgat atttttaccg ctatccttaa tagacagaga cggtcctggc
    10801 ttaaaactgc cgcccttcac ttttttccat attttattga tgttttgcca attaattaga
    10861 ccagccgaag ctaacatttg ttcggtcttt ttttctgtgt aatttaagtg ggaaatagag
    10921 ttgtgggcgg actgaaccgt gtctccaccc atatcctctt ttagctccgc taagctcaaa
    10981 ccaaaggtat tcttaataat tatacccaaa aaaggaacgt aggaattcag acgcgtaaga
    11041 ttatttttta tttgttccgc cgctaccatg ggggggagct tggtaataat attaattaaa
    11101 tcgtgattgg ataaatattc tggtaaagcc tcattacaaa aaagaaaata agaagaagca
    11161 gggatctctc cgctaattac tgaagaaaaa aatttgggcg aaaccggacc agtgctttcc
    11221 ggctgattat cggcttcaac ggcactagtt tcgacgttta ctaattcata atcatcttga
    11281 cgtttatata ttaaaaaagc tttattgcgg ccaaaattag aaaatagcaa tttattatca
    11341 aaaatcaagc ctaaaactaa attagtatcc tcagcccgta atataatttt ttccaaagaa
    11401 ataaattcca gtaaagattt attgagttta gctaaagcgg cctcaaaaat attatccaaa
    11461 ctcaaacctt caattttatc ctgtaaaaat attttctcat cgttatagta gaaatcattc
    11521 aaacgctcaa taataaaatc aataaccttt ttaacgtcat tctttttgcc gtcaatttcc
    11581 gctaataaaa ataatttacc ggctaaattt tccttaattg catccggctg aaaaataaaa
    11641 acttcccggc tgccgttctg atgcttgccg gtatttaagg taaaggaaga gattttataa
    11701 tacatataag cttaatctct tgacgccatc cgcgccaatt ttatttgact gctaaccttt
    11761 tctattatac tataaaataa gcaagaaaaa aagagataaa agcactttag aaaaaagata
    11821 aaataaaaaa acaatcttat gttggcgcaa atttttggct ctaatgctag agtaaaaata
    11881 atgaaaactt tccttagcaa gcctgaacaa aaatattata ctcgacaact agcccgagat
    11941 ctaaatcttc aggtaaattc agtgcggcga gaattgaaaa atctagaaga aatcggccta
    12001 atcaaacaag aagaggcaaa aattttatct gaggtcaaag aaacaaagat taaaaccggc
    12061 gctaaaaatc tcaaaaatga aaaaaaatat tttacgattg accgtaattt tctgcttttt
    12121 ccagaactaa aaactttatt tgctaaagcc aatctcttgt cttgtcaaga ttttttaaag
    12181 aatacggctg aatttggaga aataaaacaa ttatggctga gcgggatttt cagcggtgac
    12241 ggaagcgccc cgattgatat gttactggtg ggaaaaatca aaaaagataa atttataaaa
    12301 agactgcaaa acctagaggc tgatttaaat aaagaaataa attacaccct gatggacgaa
    12361 gctgaatatg agtatcgact agaaatccat gatatttttc taaatcgcat ccgccaaagc
    12421 cgacatttaa aaataattaa tagcgaaaaa gaaaataatt aaggacatct atgaaggtta
    12481 aagctaattt aaaatatttt gctttactaa gcctgcgtct attattatgg gcgctggcga
    12541 tttttttctt atcactcata atcagcttaa taagtagttg gtcctattgg cgagatttcg
    12601 gccaaaacgc tattgccggc aaagagcaac tgagcggagc gattgtttct attcgcgcga
    12661 aagattggga tggtgctcaa ttaaaactgc aatcggcgca agattatttt aaaaatggac
    12721 aaaaaaattt agaaaaactg cgccaatctt ggtttccggc aaaattaaaa ttaggcagcc
    12781 ggccaataga tgacttggga taccttaata atagcgctct aataattagc ggcagcgcct
    12841 tgcaggttaa ctctctactc aagaatatca gcgccgatgc ctttgctaat gagcgtaatt
    12901 tccagagtct aagtcaagaa aaaaaagccg cctttatgca atcccttatt gctcttgaac
    12961 cggagcttaa tggcttaaaa gccaacctta atctcgctct ttttaatctt gataagattc
    13021 aaactgttgg cattctttgg cctttacgcg gtcaactagc tgaactgcga aatcaaattg
    13081 ctgatggtga aaatatatta tccaattctt taccaattat ccgtttactt ccggccttta
    13141 gcggctatcc tcaaccagta cattacttaa ttatgttaca aaataatgac gaactgagac
    13201 cgactggcgg ttttttgggc tcctatgtcc gtgccgatat tactaacttc ggtgaattcc
    13261 aaaaattgga ggctaatgat atctatcatc ttgacatgcc agccattggt aaaaccgctt
    13321 ttgtcgctcc ggaaccgatt agcaaatatt taaaagttga taactggtat ttgcgcgacg
    13381 ctaactggtc gccggattgg ccgcaatcag ctaggcaaat tcaaatcatg tttcaggcag
    13441 aaagtgccgc cagcggaaaa gccgagccca atttagacgg tattttcgct atcactcccg
    13501 actttatcgc caatctatta cgcttaaccg ggccgataac ggttagggga gaaagttatg
    13561 ccccggaaaa tatgcaggca ttattacaat ataatgtgga agtcgcctac aaagaagagg
    13621 aaatttcttc ctgggatcga aaagatatta ttaatgaatt aattcaagcc ctaaaagaaa
    13681 aaattatcgc cctccctttg gaaaaatatc cagaattaat cagctgtctt aatgacagtt
    13741 taaagagggg ggacattctc ctgtatttca ataaccaaaa ccttcaaaca ttggctcaga
    13801 acctgggggg cgggggagaa attaagcaag ttagcggcga ttatctaatg gtggtcgacg
    13861 ccaacttggc cgcttttaaa agcgacgccg tgatgcagaa aaatattgcc tatcaaatcg
    13921 aaagaaagca ggagcgcttg caggcaaatt taaaattaaa ttattatcac aatggcggct
    13981 tcgactggcg caccacccac tatcgcagct acactcgcgt attagcgccc ttaggtagcg
    14041 aattaatcag attatcagga cttgaagcaa cagcagcgga ggatattatc agttatgatg
    14101 atgatagtct gaaaaaacat gttttcggtt tcttttggaa aatcgaaccg ggtaagaata
    14161 aagtgataaa tttagaatat tatttgcccg caaaactaag tgacaattta agtacaaata
    14221 atgtatacac tctttacatt caacgccaac ctggtagtcg catagctaac ttgtcttttt
    14281 cctttaatcc caataaaaac ataatacaaa taagccccca gagtctggaa ggggaaatta
    14341 agtcacaaaa agcttatttc acccttgact tgaataacgg cagccaagct atacaaataa
    14401 aaacagacta atctatggca aaatagacgt ctgttaaacc aaaaaaggaa cgatcacgaa
    14461 agttcgcgca acatataagc taggtccgct tgctcactct ttaaaagaga ggcgcaataa
    14521 tcccagccaa aactaaagcc gggcttttat ttttattata ttgactaaaa taaagaaata
    14581 tattataatt ataaaaattc tttaacaatt tgtctaacca taaatcacga taaatcatga
    14641 aaaaattaat tcttattact gttttttttg ttttttttac aatcgccagt tgggcacaaa
    14701 aactggcgaa tctctggtat ttgcccacta tgacaacata ccaagcagaa aatgtaagtc
    14761 agtacgactt actggtgatt gatcctgaaa tattagtcaa ccactcatca actcttgatg
    14821 aaatgatggc taataatcct aaattagaga tttttctcta tattaatccc accgagattt
    14881 ttgaaccgat gtggccagat aagccctgga gtattaagct ttatcaggaa ttaacaacaa
    14941 aaaaatcatg gtggttatac catccaaacg gagaaaaact cggtatctgg gcgggaatga
    15001 agactcttga tatgagagct aattgcccgg aaatagacgg gcaaagatat tggcaatgga
    15061 tattagacca atatctgaag cttttgacag ataagcgcat cagcggctgt cttatcgata
    15121 acgcctgggg cgatgaccgt gccggtatcc aatggctagc caactacaac agccaaaaag
    15181 gccttgattt aaatggtgat aagaaagctg acacaaactg gcaagaaata aatcttgcct
    15241 gggctaaagg ctttcattcc ttcttcgggg aattacgcaa agttaaagat aatctttgta
    15301 taattgctaa tcccggtaac ctgtcatacc gagaagttga cggaaagcaa tttgaaaact
    15361 tcccttatcc gcaccatcat ctaggcggtc ccgactggga agtaaacatg atgatagcaa
    15421 aaaaatataa aatagctatc attaaccctg atgaaaaaga ttttcttctt ggcgtcatca
    15481 gttccctgat gcttgacaat gcttttttgg cagttggcca aaatcagctt taccatgatt
    15541 actacaacct aaatcttggc aaagctcttg gcaaaactaa ggaaatatca aagggtattt
    15601 ggtttcgtca attcgaaaaa ggaagtatct ttatagacac caacaaaggc aacaaagctt
    15661 gggtggaata taaagatggg actaaaagag aaaaataatt tgagaaaaat aatttcaaat
    15721 aaaaaaacaa aagactcgcc taaaaaacga gtctttttct ttgattagag attttaagcg
    15781 cttaaatcag gcttcagccg ggcacaggct agagcggagc cagctaaagc ccacatttgc
    15841 cactgcaagg cggtatcatt aagaatatta tctccgctgg acatagtaaa tacggctacc
    15901 ccgaaagcca gaaaaaataa aaaaaagttc tttaaacgag gccgcgattc atgccaataa
    15961 gatgagatga tggcgccaaa gaaagacaaa agtaataaaa tatatgaagc caataatgga
    16021 taaccgccgt caagagcaac acgcaaataa tcattatggg gatcgggagg gcccaagcga
    16081 aaatccctct ttacagaaat aattttttcg gcggtgccga tgccgtagcc cgtccagggt
    16141 ctttcttgaa aatattccaa accgtcacgc cataaattaa agcgccaaga tacagaacca
    16201 taaggatctg attggaagat actgttgaat cgagtctgta tgggggggac taaagcgtaa
    16261 acaaaaagca tggctaaaaa cgcgactagt aaaaatttct taaacttaga tacgccaaca
    16321 ataacaaaaa ttataattaa agctaaataa gcgccacgag tataggttaa aaacaaaata
    16381 aaaatataaa acaacgagag cagggaatag aagtagacct ctaattttgt ccgctttaaa
    16441 tttaaaagaa gaaacaccgc taaagtaatt gcaagtaaaa gataaaaagc aagcatgttg
    16501 ggatgagtca tactgccaaa aactctataa attccatttt ctggtaaacc agtcttatta
    16561 ataaattgaa aaagagctat caatgaaggc ggcaaagcgg aaaatataat tacttttata
    16621 agccaggtaa gatttttggc tgatggcaaa aggcttgcgc ccagaaaaaa ggcggcaaat
    16681 atactaaaaa aacgcaatat ctccttcaag ctttctgaaa aataaaaaga gttaaaagtg
    16741 ctaattatcc ctaaaaatag aaaaataacc caggcataaa aggaatgcag ttgttttaaa
    16801 ttatgccaat caaactttcc caaaaatata aaaataatag ccaaaataat cattaaaatc
    16861 cccaataaac taagaatgtt aatctgcaaa tcgccaaaac tgaagatgat attatgaacg
    16921 cttaaatcta aaattggacg caaaaaaaga atagataaaa atgctaatcg aaaatcaata
    16981 aaagacaaag ccagaagcaa aaggagcaaa gacgataata aaacattagg taaagaaaga
    17041 ttggaaaaaa gcaacaacaa agcggtaaat accgctataa aagtaagggc aataactgat
    17101 tttttattat ttaatagtga gggcataaat tgaaataatc aaatatatat gctattattt
    17161 tgatattatt aatataattt tagcctaata atttcaaaag gtaaagccga gagcgatatg
    17221 aaaatacttc aagtgaataa attttattac ccacatcggg gggctgataa atattttcta
    17281 ttcttagaaa aactactaaa aagccatggt catgaagtca gggtgtttgc catgtcttca
    17341 gctaaaaatc tagaatcgcc agatgcccct tacttcgccg aagaaataga tttacataat
    17401 cttacctgga aaaacaaaat taaagccagc cgcgccatca tttataatca agaggcaaaa
    17461 agaaaattta aaaaactgct tgcggatttc cagccggatt taatccactg ccataatatc
    17521 taccaccagt tatcaccctc cattttagac gccgcccgac aagccaaaat accagttgta
    17581 atgcatctgc atgactataa actcatttgc ccaaattata ggctatatac ggaaggaagt
    17641 gcttgtcagc gctgttgtca acaacgctat tgggaatgcg tcaaacatcg ttgtctagaa
    17701 aataactttt ccaaaagcct actcgcattc atagaaatga cagttcatca ccgcttctta
    17761 aaaatttata aaaaaggagt aactctgttt attgccccca gcgagtttat gaaaaaaatc
    17821 tgtattgatt ttggctggca agacgataaa tttatagttt tagaaaatat atcaccaata
    17881 aactccacta aaataaacag tgaaattaaa gaatattttc tctacttcgg cgctttagaa
    17941 caagaaaagg ggattgattt actaataaga gccggggcgg aaagcaaacg tcccattaag
    18001 ctggcgggta caggcagggc agagcatgaa ctaagaaatt tagccctaaa attaaaggct
    18061 gatgtggaat ttttaggtca gttacaagga gataagctcc accaagtcat tgccgaatct
    18121 ttagcggtag tgataccatc acgttggccg gaaaatatgc ccctagcggc gattgaagcc
    18181 atgggcttag gaaaaacagt aattgccgca aatatcggcg gcttgagcga attaataaag
    18241 accggtgata atggctatct tttcccgcca gaagactggc aacagctggc tgaatattta
    18301 aaacaactgc aagcgccaga agctttagaa atagggaaaa aagcagcggc cagcaggaaa
    18361 gataaaacgg aagactggca ttatcaaaaa ttaatcacaa tctatcaaac tgtaatcaac
    18421 aaaaaagagc tgtaaaccca gctctttttc atatttaata ataaatttat ttatttttta
    18481 acttctaaag aaatactgta agtcaattga gagtttaacc catcaacttt aatatcataa
    18541 cggccatcat taggcactga ggggacgata acattggcct tgaatcggcc gaaacggtcg
    18601 gcctgagcgt tagttttaga gccaataagg gaaatgctta aattttcatt aggccaaaaa
    18661 ccgccgcctt gaacgctgac gctggctcct gattgagcgc tacgatttcc caacattaag
    18721 tataaatttt ccaaaggata aagctgaccg tcatcctgac taatcttatc agttttagaa
    18781 actacgttta aattagcatc taaaattaat aaaccagaat tattaaatac cactaagcga
    18841 tcgccatctg aagtgtttac cagcttcaag cccatggccc acccctgagg agcgccaaga
    18901 gtggttgcct gcacttcctt aaccaggctt aaatcacttt tctttaattt ataaacattc
    18961 aaaccattag aaaaataaag aaaattatta tcaaatgaac tttcaacatc ataagaggcg
    19021 tctccgtaat ggcgaaaaga agctaacaac ttgccgttaa aatcaaactt tttaacataa
    19081 taatcatcgg ctacaaaaat attgccgcta atacgatcat aatatgactt gcggccattt
    19141 ttcttaaaat cacggtagtc taaagaaata gttttaataa catggcggct ctcgcgatca
    19201 taaatattca aagcagaatt attaagggcg aagagaaagc gagcatcacc gctgctgcga
    19261 agactgtagg gaagttcgct ttcaaaatca aagccgtcaa ttatttgtaa atcattattc
    19321 caaatacgaa cgccgcgtcc gctgacggta gcaatgttat gaccaaaacg gtcaacccgg
    19381 tgataccact cccaataagt atttttaacc ttattttcta aactcgccgt ttgcaagtcg
    19441 gaaatatcat ataaatacaa agcgtagcca gcaacggcat aagcctttaa actctgaccc
    19501 tgaacctcaa gcttaacatc attaaaaaca tcttctttgc caggattagg agatagacta
    19561 actgacatta aatgcttaac tttgtttcca cttgaagaaa aaatttctaa tttgccagta
    19621 ttggccgtgg cgactatcag ctgtccttta tataaaacag catcgccggc gtaataagat
    19681 tgatctttag ttttaccatc gcgtacgctc cagccgacaa agagtaggcc aaaaatcaaa
    19741 gcgcttaaaa aaatcttttt catatattaa ttcgttcttt atttaactta ttattataca
    19801 tataatatat attttgtcaa gattaattat atattttgtc tattttttca aaaataaacc
    19861 caataactat ctgtttaaga taaaaaatat ttacacttat attaaatttt tatttaaaat
    19921 taaataaaaa atatataaat tatcaacatt attttaacaa ttgacatact attgacaaag
    19981 tttatataat taattataat aaatcgttat tataaagtaa ataattaata attcgtaata
    20041 tggaattaga agtattttgg aaaaacatta aaaaacgccg ccatactgtc gtcggtatta
    20101 ttttaatttc ttttatttta gtcggcacgg ctacattttt acagccttta aaatattcgg
    20161 ttaaatcaag acttttaatt tcccagaata tcagcggcgc cgatccttat actgtctcca
    20221 aatccaacca atacttaagt aatttatttt ctcaagtagt ttattcatcc tcttttttca
    20281 accttaccac ttccgccgga ttcaatattg atacctctta ttttggtaat gattataaaa
    20341 cacagatgaa agtctggaaa aagacgattg aagcccgtag cgtcggcgat accggcatta
    20401 tggaaatata tatctatcac cccaacacct atcaagccca gcaaattgcg ctggccgtaa
    20461 accaaacctt aatcagtaaa agttttaatt atcagcaggg tggcgatcag gtaatcatct
    20521 ctgttatcga ccagccttta acttccagtt tcccgataaa accaaatcta atccttaact
    20581 tcgccgccgc tctgatttta ggcattgccg ccacgagcct gtggatttat tttttcccaa
    20641 taaaagaaca taagcaccag cctaaaaaac aaaataatct tgtctatcaa gaattaccaa
    20701 cagaaaacta ttccacctat cccgccgctc cgagcaattt gccgactgaa gacaccaaag
    20761 cagataatta ctatgagcct cgaggtaata ttaataatat tttaaaataa atatttttgt
    20821 ctttttgtcg cttttttaaa ttagaaaagc ggaaaaagga cataagttct gcgtccttta
    20881 aaaactgaat aataaccaaa aatatagtac ccatgcaaaa aatatttttc ataatgatgg
    20941 cgactttatt ggcaattgcc ggtaaggaag cctccgctca gcatccattg ggggaaggtc
    21001 agttgctgat tggcaacggc tatttcgccc cagccaataa gagcggcgat ggtacctata
    21061 ggtaccttga gacccgttgg ctcccgatta aaaatcagac agataatgcc aaatttggtt
    21121 tttatttgtc tgggattgaa gtcggaagta aaatttcggg ctttaaacac cactccactg
    21181 aaattgggct cggtctggct gctaatttca gcctgcaacc cggttaccag acagacagat
    21241 acgcctggct gaaccttgcc tataaggtca tcaactcgac tggcagtctg acaaaaacag
    21301 acggccgcta tgaaaatagg caagaggacc aaatgctctt tttaggggga ggtctcctct
    21361 ttagaaacat atttcttgaa gggcccttcg cccaacaaaa gattatgtta gaatctcagt
    21421 tttccataaa aagcgaggaa aattctcgct ggaatggaga tactttggcc ggattgccgt
    21481 ggtcgcggga acgagtaaag cttcaagctg aaaatggtat tacaccgctt tatcttggct
    21541 ggagccgaaa cgtttatctc atgccttcaa tcttggctgc ctatacttac gaaaaaggca
    21601 gccaaaccag cttctatacc atcggcctta gccttacttt ggcaaagggc caatatggat
    21661 atgaaatcct gactttatcc tacgagccga agttctcagc taaaggacca agaatggatg
    21721 tgtttcaagt taacctgaat attatcaatt tataaaaatt tattaaaaat gaaaaatgaa
    21781 aagttttttt ggccatggtt gccatggatt atcgccagta tagtgtttct cgcgctattg
    21841 gtactcctta cgttccttcg cggaggaaaa aactcaacac cagtggccgc cgtggcggat
    21901 agcactaccg taacaacccc cgctgctcct tgcccaccat ttacagttgg catcaacata
    21961 agcggcgatt ctctggtgcg aatgtatcag gagattgctc gcttacaggc agcgctcaaa
    22021 gcttgtgaaa aaggaaaaaa gccggttact caggcacgta aatcatcgtc aacatcggcg
    22081 gccagcaacc agacacgcac gtcatcaaca tcggcggcga gtgcgtctca gagcactagt
    22141 ccaatgatcg ccaccaatca atacgttggt gatgtagtgg gagatgctgg ttgttcgttt
    22201 gacgaaaaca gcaagctgtt tttctatgta aaaacaagtt tgctaaatag tattaaaaac
    22261 cggacgcaaa ccacttccaa tcttaacgcc ccctctggtc caaaaggaga agaagtcggg
    22321 gaatacacct actataaaac tcccattctc atcctcaatg atatgttgga tcaggaatgg
    22381 gcttggacag tatatgttgg aaataatgcc cagtatggct acgacatgtg gctgtggcat
    22441 gagctgataa agcttaatgc cgacttaaaa aatgaccccc aaatccagag caatgggcta
    22501 ggggggtacc aatttatttc gaagattaat tatcactcta gataaattta accggtaact
    22561 ttacagttac cggttttttc ttatccaaaa aactctttag tataaaaaaa tcccctctac
    22621 acttttcaag tattgagggg aattttgtaa gctataacta catccggcgt atttcatacg
    22681 ctccagattt taagctagtt atttgtaatc ccagcatatt gtcaccggga agaaaaaatt
    22741 tagaatttgc catttcccca taaacatttg gggatgttag actggggcca aatcggaaat
    22801 aaaggtggcc ctctaaatct ttaatggcaa aagtctgata tccccaaccg gtataagcac
    22861 cggacatgat tatcagggcc atgggctgcc agttatcatt actcaacata cgagtaatga
    22921 aaggctgagg accattagcg tattgctggt aattgatata caccagcaaa gaatctgata
    22981 acggaagatt ggagtatttt atctccgttc tcaccgtggg agcataaacc ccgccaatta
    23041 ggtcgccgtt atcgccagga ataagaccgg caccaacagg attgggagac atctggccat
    23101 tagtcagata tacctcaaat actccttcac cgggagcgat taccacccaa taccttgatt
    23161 ccggagcata ggcataaacg tttcccttac cataagtaaa gcgctgtacg ccaatttcat
    23221 ttgccggcaa aattacatga tcaataatat acaaaatacc attaatcgta gtggtctcgg
    23281 ttaaatagtt gagatcccag ccggtaaaat cgccgcgatc ccaaggatca gcggcaatat
    23341 tggcataatt atacaccgcc gtagtacgca ttgcgatagc ataatcataa ttattgccag
    23401 ccactggggt agcggagagc aagataagag ttgtttccca tgttaaatca ttgctcacca
    23461 ctagggtgat actcctggtg attgaaagcc caccattaat gggtgtgatt accacgctga
    23521 tgtcatagat gccagcagcg tattttttct ccggactagt agctgtcgaa tagtcgccgc
    23581 catctaggtt ccaggagaag gtgtaattct ccactgccga cggcagggag tcaagccaaa
    23641 acttcaaaaa cacattagga gcagcgtaag cggtatcgcc gctcatattt gctccggcca
    23701 ccttcaaata aggtgccgat ttgggtgcgg aaatcggaga tgcatcgtca tactccttca
    23761 tacagcccgt aaaaataagg gctaaagcaa ataaaaacgt aaacaacgta aaccttttca
    23821 tgatatgaga tttagagtta aacttttaat tgtaaagaac gaaacctttt cgtccttatt
    23881 cttgcctcgc ttattatcaa cggggcaatt aaaagacaaa aattatttct tggctaataa
    23941 gcgattatag atcgctatcg tctgatcaac aatcttttcc caattatact cctgacgcgt
    24001 ccgttcgcgt aaagcttgtc cttgcgcttt cacaatctct tgattatcaa gtgcatactg
    24061 cagcttatga gacaaatcgg acacgtcttt attcctaaag cttaagccca gaccattcat
    24121 cgcttctaaa ttggcgggaa tatcgctggc tagacaggga aggccataag acatcgcttc
    24181 taataatccc atagacagcc cctcgtattc agaggactgg atgaacatat aggcattggc
    24241 gtaaagctcg cttaaaaggg caccgccctg attgcctaca aaaattatcc gcttatcatc
    24301 accagctaat tcttttaatt ctttaacata atcatcggta taagcaccct cgccggcaat
    24361 taccagcttc ttatcggttt tcaaattttt aaaagcctta atcacgtaat gaatgccttt
    24421 atggcgtatt aagcgggagg caataaaaat atagttgtct ttttctaggc cccagcgacg
    24481 aatttctctg acttcaggta aggtcggtgg gttgatgccc gaaggcacat aaaccgcccc
    24541 tttatcatag cgggctagag catatatagt cagttcctga gaagttacta aagtctgatc
    24601 agctaaacgg caacctatct tctcgcccaa tcggagatac cagcgcgcaa ataaacccca
    24661 ctttttatga tagtaatcct gacaatggaa agtgaaaaca ataggggtac ggggatttaa
    24721 aagcttagcc agccaaatca gggaagccgg tccaatagat tgaaaatgaa taatatcaaa
    24781 acgtctaaaa gcggaatgca aaaccgctaa aaatgtgtta gagattgcct caaaattctt
    24841 accctttata aaaggcaggg gaattatttt taccccttta tactcgctaa tttccggagc
    24901 atagccgcgc cgtgcgtaag cataaacatc atgaccgcga gcggctaagt ggattaaaag
    24961 ttcttctact tgcttttcca cgcctccagt ttgtgccggt atgccttttt gtccgatgaa
    25021 agcaattttc atatattaag tttaactaaa aattataata gttatttaaa taattgtcaa
    25081 caattgtcaa cttaataatc aaataatagt aaaaaattga tattttgtca atagtgtgtg
    25141 ctgaatatat aaaaaaataa aaggaatatt atttaaataa tattccttta atataagtta
    25201 aaaaattatt taatctaaga aaagctcaat tcaaccagtc ctttttctta aaataataaa
    25261 acatcaacca agaacctaag cccataatta ataaaataat ccaaaaacca taacgctggt
    25321 cgacaaaagg catatattta gcgttcatgc caaaaatagc cgctaacaag gttagaggaa
    25381 gaacaatcac agaaaaaaga gttaaggttt tcatgatatt attcatccgg tcgttcagta
    25441 aagactcgtt agtatcattt aaaacttcaa ccatctcttt ctgattatct aacatctccc
    25501 aaatacgttt agagtgctcc actaagtgac catactgctt cttcagagaa tctggaggga
    25561 ctagagaact gcgcatactc atcagcttct gtaaaatatt tttatgattc tgcatgatct
    25621 tgcggatatt aataatattg cggcgcaaat taagaatctt cgctactgca tctttctgct
    25681 gttgctcaaa aatcaaatct tcgacctcct ctattgccaa gctattttga tcaatcaagg
    25741 aatagcagga attaagcaaa cgatcaagaa tctcataaag caaagtagca gaagattcgg
    25801 tgttataaga aagtagagaa ttctgatctt tcttggcgtt gttaaataat tcatttaggg
    25861 ccggcaaatt gtcattatgc aaagaaacca agtagccgtg accgataaaa aattcaattt
    25921 caccggcaat aattttgcct ttaaaaaagg tgggaaagtg gaggattaga aacagataac
    25981 cattatcttc cgccaccatt ggccgctgcg aaaaaaccgt cgccacagaa gcacgtaaat
    26041 attcaagatt aaaattatat tctttcctta aatagtctat ttccttgcgt ccggcgtcaa
    26101 taatattaat ccaggacaaa gactgttcag taccgggctt atcaataagc aggcattcta
    26161 ttttctgact aatttttttg taataaccca tatagaaaga aaagtaacgg aaaataaaat
    26221 atttttgact ccgtgacttt tttgtaataa aataaggata gtttttattg cttttattat
    26281 atcagaatta acagtgagag ggaagtgatt
//
LOCUS       DAOQSA010000014        25012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_1257_length_25012_cov_9.075610, whole genome shotgun sequence.
ACCESSION   DAOQSA010000014 DAOQSA010000000
VERSION     DAOQSA010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 25012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25012
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1257_length_25012_cov_9.075610"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PKK65_02275"
     CDS             complement(<1..349)
                     /locus_tag="PKK65_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNU81541.1"
                     /translation="MSIDHKNIRVLVLALIENNGKILACPGRDTVKKQEYYRLIGGGV
                     DFGENTSSALRREIREELGLTILKPELLDVFENIFVFNGLPAHEIIFLYRVKFKSKEA
                     YRRLLFPILDSDHG"
     gene            complement(339..1196)
                     /locus_tag="PKK65_02280"
     CDS             complement(339..1196)
                     /locus_tag="PKK65_02280"
                     /inference="COORDINATES: protein motif:HMM:NF017623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81542.1"
                     /translation="MEELMFVQHDNFERQKQRLFRGGPGRLKILADFDRTLTTNFIHG
                     RKAPSLIASLRDGNYLSSDYAAKAQALHDYYQPFEDDNSLSLESKSSLMMEWWQRHFE
                     LLIASGLRQKDIVSVIDEHLANLRNGVSEFLSLLSCHKIPLIIFSASGLGRFGLKYFL
                     TRRGLWTDNIFLVANDFIWDEQGRAIGVCEPIIHSCNKNQSVLSFFNVDEAFKKRDNI
                     LLLGDSLGDAAMVDGMAAAAVLKIGFLNDKIRESLPAYRALYDVLILNDGDFALPLSI
                     LKDLLTYVD"
     gene            complement(1202..1678)
                     /locus_tag="PKK65_02285"
     CDS             complement(1202..1678)
                     /locus_tag="PKK65_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HNU81543.1"
                     /translation="MKKKDYLLLILSVAISLAAGFIGSLFTITGPGTWYDLINKPAFN
                     PPNWIFAPVWTVLYILMGLALFLVWRQGWQKLEVRKSVKMFILQLGFNVIWSYFFFGL
                     NEPHLAFLEIIALWLAIAATMIAFYRVDKRASWLLLPYLAWVSFAAFLNYSIWQLN"
     gene            complement(2118..3080)
                     /locus_tag="PKK65_02290"
     CDS             complement(2118..3080)
                     /locus_tag="PKK65_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81544.1"
                     /translation="MSDNLEQKIIEQIKQENLEPTPRWHFLLKEWLVWSVGVLSLLVG
                     AAAVSVIIYLFRNNDLFIYNQVGRSFGGWLLLSLPYFWIFFLALFVWLLFYNIKHTKR
                     GYRYPLFLIASFSVIASIFLGGVFSLFGLGERIDDVLGRQAPFYDTIFNPHINIWSHP
                     EEGRLSGLVITNFDDNNFVLVDREGEEWAVVYSGKNKGGNNIIVVGQPVRVIGRVGGE
                     SLFVAQKIMTMQPGREFFRRFKPAGVSSLNFGPGKVSPACPNDKDVFLNLLQQYPELK
                     QVLEQGLLDNQDLIREISAHNPGFLPMMQSWELFPATMQIIFSR"
     gene            complement(3088..3669)
                     /locus_tag="PKK65_02295"
     CDS             complement(3088..3669)
                     /locus_tag="PKK65_02295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNU81545.1"
                     /translation="MDNLLQISDLEDAELVRLSLADPNKFSLIINRYQDKLARYVRRL
                     GAFSAEDTEDILQDIFLKVYLNLNDFKTDLKFSSWIYRIAHNQVISHFRKHQVRPEAH
                     SSPIDSPGFELLANDISLDFQSDVNLRQAAIAAALGKIDARYREILILKFFEDKSYQE
                     ISDILKKPAGTVASLLNRAKKSLKNSLSVKMAE"
     gene            complement(3720..5228)
                     /locus_tag="PKK65_02300"
     CDS             complement(3720..5228)
                     /locus_tag="PKK65_02300"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNU81546.1"
                     /translation="MFQKFSLSNLVFSRQRHAVWRSCANPFETQTKVLRYLVDKGRQT
                     AWGRQYDYNKIKDYQSFKEQVPISTYEDLQSYIERLLHGENYVLWPEKIKYFSKSSGT
                     TGSKSKYIPVSASALRDCHYRGGRSLFATYFAQNFDSNLLLKQNFALGGSRQVDGIGA
                     DKYIADVSVILMKNLPWWAQLRRSPRFSVAAMAEWEEKLSRIAEIIAYQDIVSLSGVP
                     SWNLVLAKKILEMTGKNNLREVWPNLELFIHGGTSFVPYRSQFEALIPGKMNYLEVYN
                     ASEGFFAFQDDLSRTDMLLFLNGGVFYEFLPLSELSKESPQALTLAEVELNKSYALVI
                     STNAGLWRYLIGDTVKFTCFNPFRIVISGRTKSFINACGEELAVDNAEQAVKAAAVRC
                     QTAVKEYTAAPKFSQTTGIARHEWLFEFEQDPADLLEFSSILDEELKKLNSDYEAKRY
                     QDLNLLPPLVIRARSGLFYDWLKSRQRLGGQAKIPRLSNDRQVFDSLLALNQ"
     gene            complement(5235..6908)
                     /locus_tag="PKK65_02305"
     CDS             complement(5235..6908)
                     /locus_tag="PKK65_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNU81547.1"
                     /translation="MESNLFLQLSTILAITVSVAFFVRLAKQPLLVAYIIAGIIGGPL
                     LLNILPAEEPMYHAFANFGVVLLLFIIGLDLNFSYLKKIGKEAVIIGGLQFLLNFGML
                     LPLARSFGLSWQGAIFLALASCFSSTIVVLKILNDKQDEEVVYGRYTIGLMLVQDLIS
                     IIILLYLSFSSSDVLVGSSFWFIIKALAVIAGIILMAKYVLPEFLNKISSSGEFLFIF
                     TIAWCFGVASLLLWSGFSLEVGAVIAGLSLGSSRFHTEIASRIKPLRDFFIVIFFIIL
                     GSGADFSNLSAVLVPALFLSAFVLVFKPLILYILFRFRRFTRRNSFLAALTSGQLSEF
                     GFIILFTAASLGYLNGSEVAIFTAAAIITIFFSSYAMIFSYKIYNLFLPLFLFFGPDV
                     RIQEETKKEKFEVIIFGYHRTGWKIGQALKKKKISFAAVDFNPERAANFQHQGVRSFF
                     GDASDIEFLQSLPLDYAKTIISTIPSPEDQLVLINFVRRHNPKAIIIASLYQKKYLPS
                     LYAAGADYVILPHLLGGAWAAEVISAGALSRRSVWRQYRRQQARDLNGS"
     gene            complement(7307..7816)
                     /locus_tag="PKK65_02310"
     CDS             complement(7307..7816)
                     /locus_tag="PKK65_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81548.1"
                     /translation="MKKKNIVKASALSLATIALATGFAFSAYAQTSDTATNNQAGTGN
                     KLGRQFRMENNLTDEQKAEMEARRIEMKTKMEANRAAMQTAINSGNYDTWVAVVKAQM
                     GEQASILNQVTADNFPQFVEAHQLMNQAREKLRAIGVDDGMGFNHGPKKGMGRGLGFA
                     QQAEVADAK"
     gene            complement(8060..9286)
                     /locus_tag="PKK65_02315"
     CDS             complement(8060..9286)
                     /locus_tag="PKK65_02315"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNU81549.1"
                     /translation="MRVLIAFQLALHSLRLNLVRTSLTVLGVVIGVAAIVIVFSAGDG
                     ISRLISGEIESYGTNVVQTESKTPGKNSASSAEVTTLKLADMEAINKLDNVVSSYAAS
                     LCQQKMSYGSESKKSFVFGVSAPYAQIDTKTKLASGSFFTEEDDRSQAMVVVLGATLK
                     ENLFGEQEAVGNFIKVGTKKFQVIGVLAEQGGNTTFLNFDEMAYIPIKTLHKRILGID
                     YAMYFMHQLRDVSRAEETAEEIRALLRERHEISNPEKDDFRVSTMAEMLDTLGIVTDA
                     VTYLLLAIVLISLLVGGVGIMNIMYVTVTERTPEIGLRKAMGATAQDVAWQFLIESLL
                     ITFWGWLLGTVFGILASYGLSLLAQAFNLNWHFLFPWQGIAFSLLFSLICGLLFGYRP
                     AKQAAKLDPVSALRVE"
     gene            complement(9292..10539)
                     /locus_tag="PKK65_02320"
     CDS             complement(9292..10539)
                     /locus_tag="PKK65_02320"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNU81550.1"
                     /translation="MWENFRQLLVLVSRALLSNKTRSFLTMLGIIIGVGAVVLIMSLG
                     AGAQSLILSQLDGFGGDLIGVLPGQSDAKGPPSSVFGVKVTSLKLSDAEAFRNTKNLP
                     HLTAIVAYYQTSVSLSWRDQSYDTTLQGINGDYLELDGGELAAGRFLSKEEMSANLRL
                     AVLGSTVAAELFGGNDPIGQKIKIKNQSAIVIGVMKERGQVGFSNYDNLVFVPLSFAQ
                     KDIAGVNYLSNIKVKVDNVENIEESMEAIRQTLRERHDIDNPEDDDFSVRSFRDALDL
                     VTSITDALRYFLAAMAALSLLVGGIGIMNIMLVSVTERTREIGLRKALGATNSQVLSQ
                     FLFEAIALTLIGGLIGLLAGVFISYLAALIVRSLGYRWDFIVSLSSVLLAVGLSTLIG
                     MIFGYYPARRASRLSPIEALRYE"
     gene            complement(10543..11265)
                     /locus_tag="PKK65_02325"
     CDS             complement(10543..11265)
                     /locus_tag="PKK65_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNU81551.1"
                     /translation="MAKVLLDIKNLSKTYVSGDLVTEVLKDISFQVNTGEFVSIMGPS
                     GSGKSTLMHILGLLERSSSGIYNFAGKNIGDLNDEELAHLRNQKIGFVFQAFNLLART
                     SVLDNVKLPLIYSTNKKNMDERARKVLKSVGLDHRLSYFSNQISGGEKQRVAIARALV
                     NDPALIFADEPTGNLDSKSGVQVIQILQELNARGKTIILVTHETFTAEHAQRIIQVHD
                     GRIVSDKAVKNRRFAKDGELLK"
     gene            complement(11274..13208)
                     /locus_tag="PKK65_02330"
     CDS             complement(11274..13208)
                     /locus_tag="PKK65_02330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNU81552.1"
                     /translation="MKKSKKWSLIILALFIIGSLVWWWLAKRNQAPIYSTVTVVSGPL
                     VQTVSETGTLKPVKEVALNFLSSGRIKSVAVKVGDKVSAGTVLAALDDSSLQTKKLEA
                     EAGLQIAQASLSKILAGASTQTVAISRSTVNQAQAEVDSAKVNLEKTRQSVAENIRQA
                     EKTLSDLELNSSSTLTPSEQAVATAQTALDNAEKTGKKNVDNARNSALLFMSDKILSS
                     QIALDNINTILEDNDAKPVLSVKNSSLLAKTRDARLEALALISPANQAVNLAKSLMSE
                     AAVSSAGASVLSLLLQTDQALDYAYSMLEATITSVDFSQTKLDSYKSLISGQSSQINT
                     AISGIENSIQAFHNALLSQDTSIAVAQDNLNQAQSALSNAILAAKNNLNSLRLSGDQQ
                     IASAQARLDSANQNLALAQAQLNNTVAPARSQDVALAQAQVSQAQASLAGIEQQISDT
                     VLTAPLDGVVTAVNYEIGEQFSAGGKAMIIILVNNSFNVEVDIAESNISKVELGDDVD
                     ITFDAFPDDFILKGKVSFIEPAQTLIQDVVYYKVKIDFSDLGDTLNKVESRNLSLKAG
                     MTANIIITTDKRDEVVQVPARAIIEQDGQKIVRLLVNGVVQEQVVETGMRGDEGMIEV
                     VSGLRPGDIAITFVKNSASN"
     gene            complement(13403..13933)
                     /locus_tag="PKK65_02335"
     CDS             complement(13403..13933)
                     /locus_tag="PKK65_02335"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNU81553.1"
                     /translation="MSEIKIILKRVRRSRHLRLSIGADGSVLLTRPFWVSEKAARYFL
                     HTKADWLKAQWQKGRASDDNYFRRGGREDYLRNKEKARALVVPRLDYFNQFYNFKFRR
                     LSIRDQRSRWGSCSRNGGLNFNYRLVFLPPNLADYLIVHELCHLGEFNHSPAFWKLVA
                     RTIPDYENRRRLLRKL"
     gene            complement(13946..14815)
                     /locus_tag="PKK65_02340"
     CDS             complement(13946..14815)
                     /locus_tag="PKK65_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNU81554.1"
                     /translation="MSISLFSFPIAKKMKLNGVWLGTLKPETLYIFIHGLGGSIFSRH
                     HLISLLASGKNAVLAFNNRGFGTINYLKQGEGKNKKYFPAGAAHEVFSDCVDDIDGAV
                     AYARSQGVRRIFLLGHSTGCQKSVYYLAKRPRVRVKGAILLAPISDYSNVLRPQNRER
                     YLRALAFAKRLKESGRPHDLLPSTLWPAPLDAQRFLSLYTAESAEEIFTYASGKKPKT
                     LRAVKRPILSILAEFDEFADRPARDIKTWFDGQLKTGKYNKSALIKGIGHGFEGEERK
                     VASLIRRWSQTIK"
     gene            complement(14974..16680)
                     /locus_tag="PKK65_02345"
     CDS             complement(14974..16680)
                     /locus_tag="PKK65_02345"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNU81555.1"
                     /translation="MLKKELRSPEAKLDFSGLGIKDSILKVIFDLGLRVPTPIQHQAI
                     PAALNGEDIVGIAQTGTGKTFAFGIPMLQRLALVKGRGLILLPTRELAGQVENSLRSL
                     GGPLGLRTVSLIGGEAISRQLNALRRRPHILVATPGRLIDHLKRGSVRLDDISVLVLD
                     EADMMFDMGFAPQIEEVLKKITSQHQTLLFSATMPAAVMKLAEKHLRAPVRIEVAPAG
                     TTVELVEQEMLLLPRDSRWEQLLLLLKEFRSSVLIFVRTKHGASKLAKRLQAEKYQAA
                     EIHSNLSFPQRQAALAGFRSGQHRILVATDVAARGLDISGIELVVNFDLPDNSEDYVH
                     RIGRTARAGKKGKAVSFVAPDQIREIQKIERLIRQTLPVRLLGVSAVNKIKVKPISMI
                     EPKSLMDKKFIHQSSHKKKINQNLAPRYDSLGFRKRPDKIDPTATPDYLQPRSSNKKT
                     SFKNNFPALRPLSYSSKGMLKKTTSRVIINDSEVKPKSNNRRFENYEVTPPIVNPSFE
                     GGNRYAFRRAMYLLKEEAKGRSVKKRTSAPINGNRMERRAPNRPLRSGVRASGFKIFK
                     SS"
     gene            complement(16810..17385)
                     /locus_tag="PKK65_02350"
     CDS             complement(16810..17385)
                     /locus_tag="PKK65_02350"
                     /inference="COORDINATES: protein motif:HMM:NF015827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HNU81556.1"
                     /translation="MKNNRLAFYAVIISLVLVVILFLSFHFFFPIDLKNENGAVPIMI
                     DERLSSDVVPERLPEFTASSLPSESDVFVDPMAIEASGVAIANPTSANCVAQGGDLHI
                     QSRGDGSQFGLCYFEDNKACEEWALMNGDCPVGGRRTSGYDTEAQKYCAWLGGEVSAA
                     VDVVCSLPDGRRCLAADLYQGYDCSAGTTQN"
     gene            17501..18004
                     /locus_tag="PKK65_02355"
     CDS             17501..18004
                     /locus_tag="PKK65_02355"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HNU81557.1"
                     /translation="MQTPIRKGDSRLFAPKDYHLSPQKYEELKQKLIQLNARRPKEAA
                     EVKRLAEMGDFSENAGYQLAKGRLRDLNQRLLDLENLLKRAEIIIPDKNTDKVKIGHR
                     VTVLNEGKEKKYVILGASESDPAAGIISHNSPLGAGLLGKKINEEIKIQINGREKIYK
                     IISIEVD"
     gene            18073..18192
                     /locus_tag="PKK65_02360"
     CDS             18073..18192
                     /locus_tag="PKK65_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNU81558.1"
                     /translation="MSFTVFSQQKIGIIGRNGAGKSTLVKILDGEKLMMAAIR"
     gene            complement(18392..18769)
                     /locus_tag="PKK65_02365"
     CDS             complement(18392..18769)
                     /locus_tag="PKK65_02365"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HNU81559.1"
                     /translation="MNIYAQNILNHYQCPRHRGEMPAADASGRELNRSCGDEMTVYVK
                     IKDGLVSAISFTGKGCAISTAAISILSEELIGLSKNEILKMDIDDIRKLLGIEISWRR
                     EKCALIGLRALQGALRSLGSDKL"
     gene            complement(18771..19982)
                     /locus_tag="PKK65_02370"
     CDS             complement(18771..19982)
                     /locus_tag="PKK65_02370"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HNU81560.1"
                     /translation="MKDYRLDFPLLKRRYNDRALVYFDNAATAPKPQVVIKAVNDYYN
                     LHNANIHRGPNFLSEEATLLYEGARKKVADFIGADKEEIVFTAGATAGLNLIARAWGE
                     NNLKAGDIIALSRAEHHANIVPWLQLKEKIGVVLEYIDIKADGTFNELSLDKIFDQPR
                     LRVLAITQASNVLGQFYDLRLILKRAQAQGVLTVVDAAQSVVHNPLAVHDLDCDFLVF
                     SGHKLLAPSGVGVLYGRRDLLEKIPPFLGGGGMIAEVKEQSFTPLAEALRLEAGTPNI
                     EGAIALGAACDYLQEISWPEIIKREEVLKDYFLEQLSSLSFVRLLGGNEGRLPLFALD
                     LQGIHPHDAADLLGEKGIITRAGHHCAQPLHDSLGIGASLRASLSFYNTTAEIDVFFQ
                     ALQSIKKSFSF"
     gene            complement(19979..20725)
                     /locus_tag="PKK65_02375"
     CDS             complement(19979..20725)
                     /locus_tag="PKK65_02375"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HNU81561.1"
                     /translation="MNNFLELQTEREIKLNSGWPFLLVERWSKNSALAVRKFLLKAGV
                     EVNYLLIIDKLVDIDALERQWSIGRDTKVNLYYLFLQPLKAEIRFIHDIGAGAIVNSH
                     SLVLGNNQDDWRIKADYNFQDSGSFGRVKVEASLRGQSSLDYAASMNVSQAAQKSDTR
                     VDMRLYLEENACGQVTPGLNIAAHDVKAGHSASTFRLAPEDLFYLQSRGLSLAGIKKM
                     LSLSLAKNFIQGFPDEALSAEVLDLISTNL"
     gene            complement(20731..22128)
                     /gene="sufB"
                     /locus_tag="PKK65_02380"
     CDS             complement(20731..22128)
                     /gene="sufB"
                     /locus_tag="PKK65_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012996188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNU81562.1"
                     /translation="MMVEDLKSHNSLWDDYNQADYMARTKSGLDEVLVRRLSAEKKEP
                     AWMLAKRLESLKIFQEKPLPSWGPDLSRLNLDKITYYAKAMEGQAQSWEDVPTDIKNT
                     FDKLGIPQAEQEVLAGVGAQYESTVVYHNLKAEFKRQGVIFEDMDVALKQHPELVEPY
                     FMKAMPNTLHKFAALHGAVWSGGTFLYIPAGVRIKEPLQAYFRMNAKGMGQFEHTLII
                     VESGAQAHYIEGCSAPRYGVDSLHAGGVEIYVKEGARFRYSSVESWSKDAYNLNTKRA
                     IVEKKAHMEWVGGNFGSNTTMLYPCSILVGEGASADHLGVAFANEGQVQDTGAKVIHV
                     AKDTSSNVVMKSISKGGGKAIYRGLVEIGPQADKAAVSVKCDALILDELSASDTIPVI
                     KINNASATIAHEASAGKINEEDLFYLRSRGLKEAEAAAMLVNGFIEPITKELPLEYSV
                     EMNRMVELEMKNSVG"
     gene            complement(22131..22874)
                     /gene="sufC"
                     /locus_tag="PKK65_02385"
     CDS             complement(22131..22874)
                     /gene="sufC"
                     /locus_tag="PKK65_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNU81563.1"
                     /translation="MDLLTVKNLSVTAGNKEILHEINFSVTLGESVVIMGPNGSGKST
                     LAHVLMGHPNYQITGGQIIFQGTDISSAKPEERAAAGLFLAFQEPREIAGLELFPFLF
                     NAYKSLQVARGREAGSVFAFKKKLNDELAALDIRSDWSNRQLNQDFSGGEKKKAELLQ
                     LALAHPILAIFDEIDSGLDVDALAVAGKAIQRFKKKKNSLLAVTHYQRVLKYIKPDRV
                     LVMSSGRIVQSGGPELAARLEKEGFASVI"
     gene            complement(22879..23274)
                     /locus_tag="PKK65_02390"
     CDS             complement(22879..23274)
                     /locus_tag="PKK65_02390"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNU81564.1"
                     /translation="MFQINAKTDYGLLIMLHLAKLPGEIMPLSSLAKHLQVSSSYLSQ
                     IANSLQRAGLVKSREGVGGGYYLARPAKQIKILEILEALSGEMKVRCTHSHNKTCPHF
                     QQCGLKNAWPLLLNDIKSSLAKKNLASLL"
     gene            complement(23558..24112)
                     /locus_tag="PKK65_02395"
     CDS             complement(23558..24112)
                     /locus_tag="PKK65_02395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81565.1"
                     /translation="MQNFRGETRRLGGFGGKRDFGGRSAFGKSKSQRGVGGRDKERPT
                     MYQAVCSDCGADCEVPFRPTPGKLVFCDACFRGFDQKRQETKMPPRDNYREQLATVHI
                     KLDRILDLLQSDGFLETAVGKIKRFEDREIAPAVKKLEKVTQSAAEEIESGVKSAKKI
                     VKKVKKEAKATIVKAKKKINTLSA"
     gene            complement(24194..24934)
                     /locus_tag="PKK65_02400"
     CDS             complement(24194..24934)
                     /locus_tag="PKK65_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNU81566.1"
                     /translation="MNLENKVAIVTGAGRGIGRGIALALASAGASVVVSDVSLVDCVS
                     AAAEIKDKGGRALALPCDVADGEAVAKLMADTMAEFERIDILVNNAGIYPFKPFLEIS
                     EDDWDKVIDVNLKSIFLCSQAVAKLMPAGGKIVSISSIAAFVGFTGLTHYCASKGGIV
                     GITRALALELAEKKINVNSVAPGVIETPGTSTGMAPEAKKQTEAMIPWGRMGRPDDIA
                     QAVVFLVSPLADYITGQTLIVDGGYTLR"
BASE COUNT         8030 a         5935 c         4554 g         6493 t
ORIGIN      
        1 gaccgtggtc agaatcaaga ataggaaata aaaggcgacg ataagcctct tttgatttaa
       61 attttacccg atacaaaaat ataatttcat gtgccggcaa tccattaaaa acaaagatat
      121 tttcaaaaac gtccagtaat tccggtttta aaattgttaa acctaattcc tcccttattt
      181 ctcgacgcaa agcagatgaa gtgttttcac caaaatcaac gcccccgccg attaaacgat
      241 aatactcttg ctttttaacg gtatcccgac caggacaagc taaaattttc ccgttatttt
      301 caatcagggc taaaactaaa acacgaatat ttttatggtc aatcgacata agtaagcaaa
      361 tctttaagta tgcttaaagg caaggcgaaa tcgccatcat taagtattaa gacgtcatag
      421 agcgcgcgat aagccggcaa tgattccctg attttatcat ttaaaaaacc aattttcaaa
      481 acagccgccg ccgccatacc gtcgaccata gcggcgtcac ctaggctatc gcccaaaagg
      541 agaatattat cgcgtttttt aaaagcttca tcaacattaa agaatgataa aacactttga
      601 tttttattac aactatggat aatcggctca cagacaccga tcgcccttcc ctgctcatcc
      661 cagataaaat cattagctac taaaaaaatg ttatccgtcc aaaggccacg gcgagtcaaa
      721 aaatatttca agccaaaacg gcccaaaccg gaagcggaaa atattatcaa aggaatttta
      781 tggcaagaca ataaagataa aaattcagag acaccgttac gcagattggc taggtgttca
      841 tcaataactg aaacaatatc tttctgacgc agcccggaag caattaaaag ttcgaaatga
      901 cgttgccacc attccatcat taagctgctc ttactttcta aagacaaact attatcatcc
      961 tcaaaaggct gataataatc atgtaaagcc tgcgctttag ccgcataatc agaggaaaga
     1021 taattaccat cacgcaaaga cgctattaaa gaaggggctt tacgcccgtg tataaaatta
     1081 gtggtcagag tacgatcaaa atcagctaat attttaagtc tgccggggcc accgcgaaat
     1141 aaacgctgtt tttgacgctc aaagttatca tgttgaacaa acattaattc ctccataaaa
     1201 atcaattaag ctgccagata ctataattta aaaaagcagc aaatgatacc caggctaaat
     1261 aaggtaataa taaccaggaa gcacgtttgt caacccgata aaaagcaatc atggtagcgg
     1321 caatggctag ccaaagagca ataatctcta aaaaggctaa atgtggttca tttaagccga
     1381 aaaagaaata cgaccaaata acattaaaac caagctgaag aataaacatt tttacacttt
     1441 ttcggacttc aagcttctgc cacccttgtc gccaaactaa aaataaagcc aaacccatca
     1501 aaatatataa aaccgtccaa acgggagcaa aaatccaatt aggtggatta aaagcgggtt
     1561 tattaatcaa atcataccaa gtgccaggac cagtaatagt aaacaaagaa ccaataaatc
     1621 cggcggccaa acttatggcg acgcttaaaa taagaagtaa ataatctttt ttcttcataa
     1681 tgttacttat agttattttt attataacat aaataaaaac aaaagcgacc ggttcagata
     1741 aaagccgacg gccgctaaaa taactataat ttgatactat tctgtcttaa cctaagattg
     1801 ctcatcttaa ttaaaatttc tcccttgtga cctcacacca tccggttcat tgccaccaaa
     1861 atcgtctttc taatttgaat agcctgagca accaagccgc catcgctatt agtagcgccg
     1921 ttaacgatca cattctagtt aacggctaaa tcggggctgg cgactgcaat cgtctggcta
     1981 atatttgtct ggcggcagga gaatagttag ttgaccataa aataaaatta gcttttcttt
     2041 gatagagaat atccaaaaac agaaactttc ttccatttaa aaaacccgaa tgacaatatt
     2101 cgggttttca ttatctatta tcggctaaaa ataatctgca tcgtggctgg aaacaattcc
     2161 caagattgca tcatcggcag gaagccggga ttatgagcac taatctccct tattaagtct
     2221 tgattgtcaa gtaatccttg ctctaaaacc tgtttcaatt cgggatattg ctgcaataaa
     2281 ttcaaaaaaa cgtctttgtc gtttggacac gccggagaaa ccttaccggg accgaaattc
     2341 agcgacgaca cccctgctgg cttaaagcga cgaaaaaatt ccctgcccgg ctgcattgtc
     2401 ataattttct gagcgacaaa taaactctcg ccacccactc taccaatcac tcttacaggc
     2461 tggccgacaa ctatgatatt atttccgcct ttatttttac ctgaataaac aaccgcccat
     2521 tcttccccct ctctatcaac caagacaaaa ttattatcat caaagttagt aattaccaga
     2581 ccggataatc gcccctcttc cggatgcgac cagatattta tatggggatt aaagatagtg
     2641 tcatagaagg gcgcttggcg accgagaaca tcgtcgattc tttctcctaa accaaataaa
     2701 gaaaaaactc cccccaaaaa aatactggca ataacggaga aggaggcgat caaaaataat
     2761 ggatagcgat atccacgctt agtatgttta atattataaa atagtagcca aacaaataaa
     2821 gctaaaaaaa atatccaaaa ataaggaaga gataacaaca accagccgcc aaaactacgt
     2881 ccgacctgat tataaataaa taagtcatta ttcctaaaca aataaattat cacagagaca
     2941 gcggccgccc ctactaacag agacaagacg ccaactgacc agacgagcca ctcttttagc
     3001 agaaaatgcc aacgcggcgt cggctctaaa ttctcttgtt ttatttgttc aattatcttt
     3061 tgttctaaat tatcagacat atatttatta ttccgccatt ttgactgaca aggaattttt
     3121 tagagatttc ttagcacgat tcaacaaaga agcgactgtt ccggctggtt tttttaaaat
     3181 atcagaaatt tcctgataac ttttatcttc aaaaaatttt aaaatcaaaa tttcacgata
     3241 acgcgcatca atcttgccca gggcggcggc aatagccgcc tgacgtaaat tcacatcaga
     3301 ctgaaagtca agagaaatat cgttggctaa aagctcaaaa ccggggctat caatggggga
     3361 tgaatgcgct tccgggcgca cttgatgctt gcgaaaatgg ctaataactt gattatgggc
     3421 aatccgatat atccaagagg aaaatttcaa atcagtctta aaatcattaa ggttaagata
     3481 aactttcagg aagatgtcct gtaaaatgtc ttcagtgtcc tcggcgctaa aagcacctaa
     3541 acgccggaca taacgggcga gcttatcctg ataacgattg atgataagag aaaacttatt
     3601 cggatcggcc aaactcagcc tgaccagttc cgcgtcttcc aaatctgaaa tttgaagcag
     3661 attgtccatg gggcatgata tgtatataaa tacgcctaaa aaaatatttt cttccttacc
     3721 tattgattta aagctaataa tgaatcaaat acttggcgat cattagacaa acggggtatt
     3781 tttgcctgcc caccaagacg ctgtctagat ttcagccaat cataaaaaag accggaacga
     3841 gccctgatta ccagtggcgg caataaattt aaatcctgat agcgcttcgc ttcataatca
     3901 gaattaagtt tctttaattc ttcgtccaaa atcgaggaaa attccagcaa atcagccgga
     3961 tcttgttcaa attcaaaaag ccactcgtga cgggcgattc cggttgtttg cgaaaattta
     4021 ggcgccgccg tatactcctt gaccgccgtc tggcaacgga cggcagccgc cttcaccgcc
     4081 tgttcggcat tatccaccgc caattcttca ccacaggcat taatgaaact ttttgttctt
     4141 cctgaaatta caatccgaaa aggattaaaa caagtaaatt taacggtatc acctattaaa
     4201 taacgccaga gaccggcatt ggtagaaata actaaggcat aacttttatt caactccacc
     4261 tccgccaaag tcagtgcctg cggtgactcc ttggataatt ctgacaaagg caaaaattca
     4321 taaaaaaccc caccgtttaa aaacaagagc atatcggtac gcgacaaatc gtcttgaaaa
     4381 gcaaaaaatc cctccgaagc attataaact tcaagataat tcatttttcc aggaatcaaa
     4441 gcttcaaact gagaacgata aggaacaaaa ctggtgccac catggataaa taattccaaa
     4501 ttaggccaaa cctctcgcag attattttta ccagtcattt ctaatatttt tttagccaac
     4561 accaaattcc aagaaggaac accggaaaga ctaacaatat cttgataggc aataatttct
     4621 gcaatacggc tgagtttttc ttcccattcg gccatggcgg ccacactgaa acgaggactc
     4681 cgcctcaact gcgcccacca aggtaaattt ttcatcaaaa taacagaaac atcagcaata
     4741 tacttatccg cgccaatacc atcaacttga cgactaccgc ccaaagcgaa attctgcttc
     4801 aatagtaaat tagaatcaaa attctgagca aaataagtag caaaaagact gcgaccgccg
     4861 cggtaatggc aatcgcgcaa agcgctagcg cttaccggga tgtacttgct cttactaccg
     4921 gtagtgccgg aagatttgga aaaatattta atcttttccg gccaaagaac ataattttct
     4981 ccgtgtaaaa ggcgctcgat ataactttgt aagtcctcat aagtgctgat agggacttgc
     5041 tccttaaaag attggtagtc tttaatttta ttataatcgt attgccgacc ccaagcggtc
     5101 tggcgtcctt tgtcaactaa ataacgaagc actttggttt gagtctcaaa aggattggcg
     5161 caagaacgcc atactgcgtg acgttgtctg gaaaaaacga gatttgacaa agaaaatttt
     5221 tgaaacatag tcatttaact accgttaaga tcacgggctt gctggcggcg atactgacgc
     5281 caaactgaac gccgagatag ggcgccagcg gaaattactt cagccgccca agcaccaccc
     5341 aataaatggg gtaaaatgac gtaatcggcg ccagctgcat ataaagatgg cagatatttt
     5401 ttttgataca agctggcgat aataatcgct ttagggttgt gccggcgcac aaaattgatt
     5461 aataccagct gatcttcggg agaaggaatg gtagaaataa tcgtcttcgc atagtcaagg
     5521 ggtaatgatt gtaaaaactc aatatcggaa gcgtcgccaa agaaagaacg aactccttgg
     5581 tgttgaaaat tcgccgctct ttctggatta aaatcaacgg cggcgaagct tatctttttc
     5641 ttttttaaag cctgaccgat tttccaacca gtgcggtgat agccgaaaat gatcacctca
     5701 aacttttctt ttttggtctc ctcttgaata cgaacgtcag gtccaaaaaa caaaaatagg
     5761 ggcaaaaata aattataaat cttataacta aaaatcatgg cgtatgagga aaaaaagata
     5821 gtaataattg ccgccgccgt aaatatcgcc acctcactgc cattaagata gcccaaagaa
     5881 gcggcggtaa ataaaataat aaaaccgaat tcactcaact gtccggaagt taaagcggct
     5941 aaaaagctat tacgtctagt gaaacgacgg aacctaaaaa gaatataaag aatcaatggt
     6001 ttaaatacta aaacaaaggc cgacaaaaat aaggcaggga caagaacggc tgataaatta
     6061 ctaaagtcgg cgccactgcc aagaataata aaaaaaataa caataaaaaa atcccgtaat
     6121 ggtttaatac gactggctat ttctgtgtga aaccgagaag aacccaaact caaaccggca
     6181 atcaccgccc ccacctccaa agaaaatcca ctccaaagaa gtaaagaagc aaccccaaaa
     6241 caccaagcga tcgtaaaaat aaacaaaaat tccccagaag aagagatttt atttaaaaat
     6301 tccggcaaaa catatttagc catcaaaata ataccggcaa tcaccgctaa ggctttaata
     6361 ataaaccaaa aagaactacc aactaatacg tcagaagaag aaaagctaag ataaagcaaa
     6421 atgataatag aaataagatc ctggacaagc atcaagccga tagtataacg tccataaaca
     6481 acttcttcat cttgtttgtc atttaaaatt tttaagacga caatggtgct ggaaaaacaa
     6541 gaagccaaag ctaaaaatat tgctccctgc cagcttaaac caaaactacg agctaaaggt
     6601 aaaagcatgc cgaaatttag aagaaattga agtccgccaa taataaccgc ctccttgcct
     6661 attttcttta aataggaaaa atttaaatct agaccaataa taaaaagtaa gaggacaacg
     6721 ccaaaattgg caaaagcatg atacatcggc tcttcagccg gtaaaatatt caaaagcaag
     6781 ggaccgccaa taatgccagc gataatataa gccaccaaca aaggctgctt ggctaaacgt
     6841 acaaaaaaag ccacgctgac agtgatggct aaaatagtac tgagctgtaa aaaaagatta
     6901 gattccatgg atgataacta aaatagataa tataataaat tataacatat aaacaaagcc
     6961 ttcaacaaat taacgaatca cattcgcagt agttttaatc agtaaaaaaa ttagatcagt
     7021 agccacggaa aatctcacca gctcaatctt ccttctcttc gctaccgtta tttccgttct
     7081 cctattcata ttagctaaaa aatggaatat tttttaagta ctctagtcta ataaaataac
     7141 cttctccccg ccctcactag ctactcatgc ggagtttcct ggccttataa aaacggcgat
     7201 aaaagccgtt ttttattgac taacaaaaga gcctcttctt cggagactct tttgtctgaa
     7261 tgaaataatt aaccgctgct cagctgatta actgtatcac tattgattat ttagcatcag
     7321 ccacctccgc ctgctgggcg aaacccaagc cgcgacccat gccttttttc gggccatgat
     7381 tgaaacccat accatcatcg acgccgatag cccgtaattt ctctctagct tgattcatta
     7441 attgatgagc ctcaacgaat tgaggaaaat tatccgctgt cacctggttt agaattgaag
     7501 cctgctcgcc catttgcgct ttcacaacag ctacccaagt atcgtaattt ccactattaa
     7561 tagctgtctg catcgcggcg cgattagcct ccatctttgt tttcatctct atccgtctcg
     7621 cttccatttc tgctttctgc tcatcagtta aattattttc cattctgaac tggcgaccta
     7681 atttattacc agtaccagcc tgattgttgg tagcagtatc actggtttga gcataagcgc
     7741 tgaaagcgaa accggtggct aaagcgatgg tggccaggct caaagcacta gccttaacaa
     7801 tatttttctt tttcatattt ttatgatttt ttctcccaca gctatcactg aaagcggctg
     7861 aagttgaaaa tatttttaaa tttataaagt caataggtcg ggcgcctcta acatttaata
     7921 cgcatctttt catattttct tgcaatcttt tatcaagagc aatgatgatc ttctccccgc
     7981 cctcactggc agcctatgtg gggtttcctt gccttaaaaa tgcctatcat taagacattt
     8041 tcatctgtaa atcatggtat tattcaacac gcaaagccga taccggatca agtttggctg
     8101 cctgcttagc cgggcggtag ccaaaaagca aaccgcaaat aagagaaaaa agtaaagaaa
     8161 aagctatccc ttgccaagga aaaagaaaat gccaattaag attgaaagcc tgagccagta
     8221 aactgaggcc ataagaagcc aaaatgccaa aaaccgtgcc aagtaaccag ccccaaaaag
     8281 taattagtaa agattcaatt aaaaactgcc aggccacatc ttgagcggtt gcccccatcg
     8341 ctttacgcaa tccaatttcc ggagtccttt cggtcacggt cacatacatt atattcataa
     8401 taccaacacc gcccactaat aaagaaataa gcacgatggc cagcaagaga taagtaacgg
     8461 catcggtgac gataccgaga gtgtcaagca tttctgccat tgttgacact ctaaaatcat
     8521 ctttctcagg atttgaaatt tcatgccgtt cgcgcaaaag agctcggatt tcctcggccg
     8581 tttcttctgc tcggctaaca tcgcgcaact ggtgcataaa atacatggcg taatctatac
     8641 ctaaaattcg cttatgtaaa gtcttgatag gtatataggc catctcgtca aaatttaaga
     8701 aagttgtgtt tcctccttgc tcagccagca ccccgataac ttgaaatttt ttcgtgccga
     8761 ctttaataaa attaccgaca gcttcctgct cgccaaataa attttccttc aaagtcgccc
     8821 ctaacaccac taccatcgcc tgactgcggt cgtcttcttc tgtaaaaaaa gaaccagacg
     8881 ccaattttgt tttagtatct atctgcgcat aaggcgcgct cactccaaag acaaaacttt
     8941 tcttactttc cgagccataa gacattttct gctggcacaa agaagcagca tagctactaa
     9001 cgacattatc gagcttgtta atcgcttcca tgtctgctag cttcaaagtt gtaacctcgg
     9061 ccgaactggc gctatttttc cccggcgttt tcgattccgt ctgcacgaca ttggtgccat
     9121 aagattctat ttctccggaa ataagacggc taatgccgtc accggccgaa aaaacaatga
     9181 cgatagcagc cacgccgata accaccccta aaaccgtcaa gctggtacgg accagattaa
     9241 gacgaaggga atgtaaagca agctgaaaag cgattaatac gcgcataaaa attattcgta
     9301 acgcaaagct tcaatcgggc ttaaacgact ggcgcggcgc gccggataat aaccaaaaat
     9361 cataccaata agagttgata acccaacagc tagcaaaaca gaagacaaag aaacgataaa
     9421 atcccagcga tagccgagag aacgaacaat taaagcggct aaataggaaa taaaaacacc
     9481 tgccaataat ccaattaaac cgccgattaa agtcaaagca atcgcctcaa ataaaaattg
     9541 gctaagaacc tggctattag ttgctcccaa tgccttgcgc aatccgatct cccgagtacg
     9601 ctcagtaaca ctcactagca tgatattcat aatgccgatt ccgcctacca gcaaagacaa
     9661 ggcggccatc gccgccaaga aataacgcaa agcatcggta atactggtaa ctaaatccaa
     9721 agcatcgcgg aaactccgga cagaaaaatc atcatcttcc ggattatcaa tatcgtgtct
     9781 ttcgcgcaaa gtctgacgaa tcgcctccat actttcttcg atattttcca cattatcaac
     9841 cttaacctta atattgctta aataattaac gccagcaata tctttctgag caaaagacaa
     9901 gggcacaaaa actaaattat cataattaga aaaaccgacc tgccctcttt ccttcattac
     9961 accgataaca atcgcacttt gattttttat ctttatcttc tggccaatag gatcgttgcc
    10021 gccaaaaagt tcagcagcaa ccgtactacc aagaaccgct agccgtaaat tagccgacat
    10081 ctcttctttg cttaaaaaac gtccagcggc aagttctcca ccgtcaagtt ccaaataatc
    10141 cccgtttatt ccttgtaagg tcgtatcata actttgatcg cgccaagaca aggaaacgct
    10201 agtttggtaa taagcaacta ttgccgtgag atgaggcaga ttctttgtat tacgaaaagc
    10261 ctcagcatca ctcagcttca aactagtaac tttaacccca aaaacggaag atggcggtcc
    10321 cttggcgtcc gactgtcccg gtaaaacgcc tataaggtcg ccgccgaaac catccaactg
    10381 actcaaaatt aaagactgag caccggctcc taaagacata atcaagacaa cggcaccgac
    10441 gccgataata attccgagca tcgttaaaaa actccgggtc ttattgctca acaaggcccg
    10501 ggaaactaaa accaaaagct gtcggaaatt ttcccacata aatcacttca aaagttcacc
    10561 atctttggca aaacgacggt ttttaaccgc cttatcactg acaatcctgc cgtcatgaac
    10621 ctggataatg cgctgagcat gttcagccgt aaaggtttca tgagtcacta atataatagt
    10681 cttacctcta gcatttagtt cctgcaatat ctgtataacc tgaacaccag attttgaatc
    10741 aagatttcct gtcggctcat cagcaaaaat tagagccgga tcattgacta aagcgcgagc
    10801 aattgccacg cgctgcttct ctcctccgga aatctggttg gaaaaataag aaagacgatg
    10861 atcaagaccg acgctcttta aaactttacg cgcccgctca tccatatttt ttttgttagt
    10921 tgaatagatt aaaggcaact tcacgttatc aagcacactg gtacgggcca gaagattaaa
    10981 agcctgaaaa acgaaaccta ttttttgatt acgcaaatgc gccaattctt catcattcaa
    11041 atcaccaatg ttcttgccgg caaaattata aataccactg cttgaacgct ccaagagacc
    11101 taaaatatgc attagggtag acttgccgga accagatggg cccataatag aaacgaattc
    11161 ccctgtattt acttgaaaac ttatatcttt taaaacttca gtcaccaaat cgccactaac
    11221 ataagtcttg gagagatttt ttatatccaa taaaactttg gccataattc ttattaatta
    11281 gaggcgctat ttttaacaaa tgtaatcgct atatcgcccg gtcttaaacc gctaactacc
    11341 tctatcatgc cctcgtctcc gcgcatgcca gtttccacta cttgctcttg caccactccg
    11401 ttaaccagca aacgtacaat tttctgcccg tcttgttcaa taatggcccg agccggaact
    11461 tgcacaacct catcacgctt atcggtggta ataataatat tagcagtcat acccgccttt
    11521 aaagataaat tccgactctc aaccttattt aaagtatctc ctaaatcaga aaaatcaatt
    11581 tttactttat aataaaccac gtcttgaata agggtctgtg ccggttcaat gaagctaact
    11641 ttgcctttta aaataaaatc atcaggaaaa gcgtcaaaag taatatcaac atcatcacca
    11701 agctcgacct tgctgatatt tgattcagca atatctactt caacattaaa actattattt
    11761 actaaaatta taatcatcgc ttttccgcca gcgctgaact gttctccaat ttcataatta
    11821 accgccgtca caacaccgtc taaaggggcg gttaaaactg tatcgctaat ctgttgttca
    11881 atgccggcca gactggcttg agcctgactg acctgggctt gcgctaaagc gacatcctga
    11941 gaacgtgccg gagcaacagt gttattaagc tgggcttgcg ctaaagctaa attttgatta
    12001 gcactatcta aacgagcttg agcagaagca atttgctgat cgccacttaa gcgcaaacta
    12061 tttaaattat ttttagcggc caagatggca ttacttagag cagattgtgc ctgatttaaa
    12121 ttatcttgtg cgactgctat cgaggtgtct tgactaagta gagcgttatg aaaagcctga
    12181 atagaatttt caataccgct aatagccgta ttaatttggg aagactggcc actaataagg
    12241 ctcttatatg aatccaattt tgtttgagaa aaatcaacac tggtaatagt agcctctaac
    12301 atagaataag cataatccag agcctgatct gtttgtaaca aaagagacaa gacggaggcg
    12361 ccagcactac tgacagctgc ttcactcatc aatgacttcg ccaaattaac ggcttgatta
    12421 gctggagaaa ttaaagctaa agcttccaga cgggcgtcgc gggttttagc caaaagcgaa
    12481 ctattcttta cactcaagac aggtttagca tcattatcct caagaatagt attgatatta
    12541 tccaaagcga tttgtgagct taatatctta tcgctcataa acaaaagcgc ggaattgcgg
    12601 gcattgtcaa cattcttttt gccagtcttc tcggcattat ccaaggcggt ctgtgctgtc
    12661 gccaccgctt gctcggatgg agttaaagtt gaacttgaat taagctccaa atcagataaa
    12721 gtcttttctg cttgacggat gttttccgct acgctttggc gcgtcttttc caaattgacc
    12781 ttagcggaat ctacctcggc ctgtgcctga ttaacggtag aacgactgat tgccaccgtc
    12841 tgggtgctag cgccagccaa aattttagac aaagatgctt gggcaatctg caaacctgct
    12901 tccgcttcta gctttttagt ctgcagagaa ctatcgtcaa gagcggccag aacagtgcca
    12961 gcactaactt tatcgcccac ctttactgcc accgacttaa tccggccgga tgataaaaaa
    13021 ttcaaggcta cctcttttac cggcttgaga gtacctgttt cactgacagt ctgtactaaa
    13081 ggaccggaaa caaccgttac cgtcgaatag ataggagcct gattgcgttt agctagccac
    13141 caccagacaa gggagccaat aataaacaag gctaaaatta ttaatgacca tttttttgat
    13201 tttttcataa aaacaaacaa aatataaata tctcaatctg tatttattat aacagaaaaa
    13261 caatcaggaa ggaaaaaaga aaattacact gattgctggt ctagagatta aaaactgctc
    13321 cttataacaa tgttcacctt aaaaagacca atattttctg ttaaacaaat cggtaatcct
    13381 tttgtgttga atttcaagtt ttttacaact tacgcaataa acggcggcga ttttcataat
    13441 caggaatcgt ccgcgctacc agtttccaga aagccgggct atggttaaat tctcccagat
    13501 ggcagagctc atgtacaatc agataatctg ccaaatttgg aggcaagaac actaaccggt
    13561 agttaaaatt aaggccgcca tttcttgaac aacttcccca gcggctacgc tgatcgcgaa
    13621 tacttaaacg tctaaattta aaattataga actgattaaa ataatcaagc cgaggaacta
    13681 ccaaggcgcg cgccttttcc ttgttgcgca aataatcttc tcgaccaccg cggcgaaaat
    13741 aattatcatc actggctcgc cctttctgcc attgcgcttt tagccaatct gcttttgtat
    13801 gtaaaaaata acgagccgct ttttccgaga cccaaaaagg tctggttaat aaaacgctcc
    13861 cgtccgcgcc gatactaagg cgtaaatgac gggaacggcg aaccctcttc aatattatct
    13921 taatttcaga catcacaata aaaaattatt taatcgtctg cgaccagcga cgaataagag
    13981 aagcgacctt tctttcttca ccctcaaaac catggccgat acccttaatc aaagcactct
    14041 tgttatactt ccctgtcttt agctgcccat caaaccaagt ttttatgtcc cgtgccggcc
    14101 gatcggcaaa ttcgtcaaat tcagccagaa tgctcaatat cggccttttt accgcccgca
    14161 aagtcttagg ttttttaccg gaagcataag taaatatttc ttcggcgctc tcggctgtat
    14221 ataaactaag aaaacgctgg gcgtcgagcg gtgccggcca aagagtggac ggtaataaat
    14281 catgaggacg cccgctttcc tttaatcttt tagcaaaagc caaagcgcga agatatcttt
    14341 ccctattttg gggacgtaac acattagaat aatcgctaat cggcgccaat aatatcgccc
    14401 ccttaactct tactcgtggc ctcttcgcta aataataaac actcttctga caaccggtgg
    14461 aatggccgag cagaaaaata cgtctaactc cttgagagcg agcataagcg accgcgccat
    14521 caatatcatc aacgcaatcg ctaaaaactt catgcgccgc gccagcggga aaatattttt
    14581 tgtttttccc ttctccttgc ttcaaataat taattgtacc gaaaccgcga ttattaaaag
    14641 ccaaaacagc gtttttccct gaagccaata atgaaattaa atgatgccgc gaaaaaatac
    14701 ttccgcccaa gccatggata aaaatataaa gtgtttctgg cttgagtgtt cccaaccaaa
    14761 cgccgttaag cttcatcttt ttggctatag ggaaagaaaa taaagagata gacataaaaa
    14821 atgcttaaac aaattgaatc ttaatattat aaagcattat ttctaattag gctacaaaca
    14881 aaaaaccgcc gttgttaaag cgccgatttt ttaataccta tttttcttaa aacgaggacg
    14941 gaaaaatttg gccttaaaag caggacggtg agactacgag cttttaaaaa tcttgaagcc
    15001 ggaagcgcgc accccggacc gaagcggtct attgggtgca cgacgctcca ttcggttccc
    15061 attaatggga gccgacgttc tctttttcac agaacggcct ttagcctctt cctttaaaag
    15121 atacatggcg cgccggaaag catagcgatt acccccctca aaagacgggt tgacaattgg
    15181 cggcgtcacc tcgtaatttt caaaacgacg attattagac ttaggtttaa cctcagaatc
    15241 gttaataatt actcgtgaag tcgttttttt caacatcccc ttactagaat aagataaggg
    15301 acgcagggct gggaaattat ttttaaaact agttttttta ttagaactcc gaggctgcaa
    15361 ataatcaggc gtggccgtcg gatcaatttt gtcaggacgt ttgcgaaaac cgaggctatc
    15421 atagcgagga gcaaggtttt gattaatctt ttttttatgt gaggactgat gaataaattt
    15481 tttgtccatc aatgatttgg gctcaatcat agatataggc ttaactttaa tcttattaac
    15541 agccgacacg ccgaggaggc gtacaggtag agtttggcga attaaacgct caatcttttg
    15601 aatttctcta atctgatcag gagccacgaa agaaacggct ttaccctttt taccggcacg
    15661 ggctgtccga ccgatgcgat gaacataatc ttcggaatta tcaggtaaat cgaaattaac
    15721 aaccaattca atgccgctaa tatctaaacc acgagcagcc acgtctgtcg ccactaagat
    15781 tcgatgctgg ccactgcgaa aaccggccaa agccgcctgc ctttgcggga aggaaagatt
    15841 agaatgaatt tcggcggctt gatatttttc agcttgtaat cttttagcta attttgaggc
    15901 gccgtgcttg gtgcgaacaa atatcaagac cgaagaccga aattctttca ataacaaaag
    15961 taattgctcc caacggctat cgcgcggtag aagcaacatc tcttgttcca ctaactctac
    16021 tgtcgtccca gccggagcaa cctcaatcct taccggcgcc cgcaggtgtt tctccgctaa
    16081 tttcatcacc gccgccggca tagtagcgga aaataaaaga gtctggtgct gagatgtaat
    16141 tttttttaaa acttcttcaa tctgcggcgc aaagcccatg tcaaacatca tgtccgcctc
    16201 gtccaaaacc aaaacggaaa tatcatccaa gcgaacggag ccgcgcttaa gatgatcaat
    16261 taatcgcccc ggagtcgcga ctaaaatatg agggcgacga cgcaaagcgt taagctgacg
    16321 gctgatggct tcacccccga ttaaagacac cgtacgcaaa cccaaaggcc cgcctaggct
    16381 gcgcaagctg ttttctacct gtcccgccaa ttctcgagtc ggcaatagaa tcagaccgcg
    16441 acctttaaca agcgccaagc gctgaagcat gggaatacca aaagcaaaag tcttgccggt
    16501 acctgtctga gcaataccga caatatcttc accatttaaa gcggcgggaa tagcttgatg
    16561 ctggataggg gtaggcaccc ttaaacctaa atcaaaaatc acttttaaaa ttgaatcctt
    16621 tatacccaaa ccggagaaat ctaacttagc ttccggagag cgcaattctt tctttaacat
    16681 aaaaaaataa gcggccgtca agccactgcg acgattataa aataaaaaga tagagaagca
    16741 tatcttgttt ttaatataac acaaaaactc aatataagtc aaagcaaaaa ttttagaaaa
    16801 atgttttggc taattttgag tggttccggc cgaacaatcg tagccttgat aaagatcagc
    16861 ggctaaacag cgacgaccat caggtaaaga acaaacgaca tctacagccg ccgacacctc
    16921 ccctcccagc caagcgcaat atttctgcgc ctccgtatca taaccactag tacgccggcc
    16981 accgacagga caatcgccat tcatcaatgc ccattcttcg caagctttat tatcttcaaa
    17041 ataacaaagg ccaaactgag aaccatcacc tcgactttgg atgtgtaaat ctccgccttg
    17101 tgccacgcaa ttagcggagg tggggttagc aatagcgacg ccgctggctt caatcgccat
    17161 cggatcaaca aaaacatcgc tttccgaggg gagactagaa gcggtaaact caggtagcct
    17221 ttccggaaca acatcgctag ataaccgctc atcaatcatt attggcacgg cgccattttc
    17281 attttttaaa tcaatcggaa agaaaaaatg gaaagataag aataaaataa caaccaaaac
    17341 cagcgagatg atgactgcat aaaaagccag acgattattt ttcataatga ttatcaattt
    17401 tttatttata attttcacat ctttttaaaa atcataaaca caaacaaaat attgcctttc
    17461 agagacacat agcttatgat gaagctataa tatcaatctt atgcaaacac ctatccgcaa
    17521 aggcgacagc cgcctctttg cccccaaaga ttatcacctc agccctcaaa aatatgagga
    17581 actaaaacaa aaacttattc aactcaacgc cagaagacca aaagaagccg ccgaagtgaa
    17641 aaggttggcg gaaatgggcg acttttcgga aaatgccggc taccaactgg ctaaagggcg
    17701 cctgcgcgac ctaaaccaac gcctgcttga tttagaaaat ctgcttaagc gcgccgaaat
    17761 cattatcccg gataaaaata ccgacaaggt taaaatcgga catcgtgtca cggttttaaa
    17821 tgaaggcaaa gaaaaaaaat atgtcatcct cggtgccagc gaaagcgacc cggcggctgg
    17881 catcatctcc cataattctc cgctcggcgc cggcctgctc ggcaaaaaaa ttaacgaaga
    17941 aataaaaata caaattaatg gccgggaaaa aatctataaa atcatcagta tcgaagttga
    18001 ctaaatacgt ccatactaat acaaaccagt aatctcaatt aagaaggcaa tgaccaaaag
    18061 attttcagcg gtttgtcttt tactgtattt agccaacaaa aaatcggcat tatcggtaga
    18121 aatggcgccg gcaaatcaac tcttgttaaa atcctggacg gtgaaaaact aatgatggca
    18181 gcaatacgat gattgagtgg agttgcgcaa gaaccgcata aatatatggc ttgcgcaccg
    18241 atagcagcgg ctaaacgtca aaaccgccga tattataagt atctaaaata tcttcccaaa
    18301 taaaatatgc gtctggtcaa agtttttaat tagatatttt gacgcctcaa acggcgataa
    18361 gcgacgcaac tctcgggcat ctataaccat atcacaattt atcgctccct aaagaccgca
    18421 gagccccctg caaggcgcgc agaccgataa gcgcacactt ttcccgccgc caactgattt
    18481 caatccctaa taatttacgg atatcatcaa tgtccatttt taaaatttca ttcttgctta
    18541 aaccgattaa ttcttcactc aaaatagaaa tagcggccgt agaaatagca cagcctttac
    18601 cggtaaaaga aatggcgctt actaagccgt cttttatttt tacataaaca gtcatctcgt
    18661 cgccgcagct gcgattaagc tcgcggccag aggcatcagc agcgggcatt tctcctcgat
    18721 ggcgcggaca ttggtaatga ttaagaatat tttgagcgta aatattcata ttaaaaagaa
    18781 aaagattttt taatagactg taaggcctgg aaaaaaacat caatttccgc ggtcgtatta
    18841 tagaaagaca ggctggcgcg caggctggca ccgattccta aagaatcgtg caaaggttgg
    18901 gcgcaatgat gaccagcgcg ggtgataatt cccttttcac caagcaagtc agcggcatca
    18961 tgcggatgaa tgccttgcaa gtccaaagca aatagaggta gtctgccttc attgccgccc
    19021 agcaagcgga caaaagataa agacgataat tgttctaaaa aataatcttt taagacttcc
    19081 tcacgcttga ttatttccgg ccaggaaatt tcttgtaaat agtcgcacgc cgctcccaaa
    19141 gcgatcgccc cctcgatatt gggcgtaccg gcctccagtc ttagggcctc cgccaaagga
    19201 gtgaaagact gctctttcac ttcggcaatc atcccgccgc cacccaaaaa tggcggtatt
    19261 ttttccaata aatcccggcg tccataaagc acgccgacac cagacggagc taaaagctta
    19321 tgtccggaaa aaaccaaaaa atcacaatct aaatcgtgga cagccaacgg attatggacg
    19381 acgctttgag cggcatcaac aaccgtcaac actccctgcg cttgcgctcg ctttaatatc
    19441 aaccgtaaat cataaaactg accaagaaca ttagaggctt gtgtaatcgc caaaacacgt
    19501 aaacgaggtt gatcaaaaat tttatccagg ctaagctcgt taaaagtgcc gtcagcctta
    19561 atatcaatat attctaaaac aacaccgatt ttttccttta actgcagcca gggaacgata
    19621 ttagcgtgat gctcggcccg gctcaaagca ataatatcgc cagcttttaa attattttca
    19681 ccccaagccc tagcgataag attgagacca gcggtcgcac cggcggtaaa gacaatttcc
    19741 tctttatccg ccccaataaa atcagctacc ttctttctgg caccttcata caacagagtc
    19801 gcttcctccg ataaaaaatt aggaccgcga tgaatattgg cgttatgtaa attataataa
    19861 tcattaacag ctttaatcac cacctgcggt ttgggcgcgg tggcggcatt atcaaaatat
    19921 accagagccc gatcattgta cctcctctta agcaaaggaa aatctaaacg ataatctttc
    19981 ataaattagt cgagattaaa tccaaaactt cggcgcttaa agcttcatcg ggaaatccct
    20041 ggataaaatt tttggccaga gacaagctca acattttttt aattcccgcc aaagacaagc
    20101 cccggctttg caagtaaaac aaatcttccg gcgccagtct gaaagtgcta gcgctgtgcc
    20161 cggctttgac gtcatgagcg gcgatgttaa gtccgggagt aacttgcccg cacgcattct
    20221 cttccaaata taaacgcata tcaactctag tatcgctttt ctgcgccgcc tgagaaacat
    20281 tcatgctagc cgcataatcc aaagatgatt gcccgcgcaa agaagcctca actttaacgc
    20341 ggccgaaact gccgctgtct tgaaaattat aatcagcttt gatacgccaa tcgtcttgat
    20401 tatttcccaa aaccaaagaa tgactattaa caatggcgcc agcaccgata tcatggataa
    20461 atctaatttc cgctttcagc ggctgtaaaa aaagataata taaatttacc ttggtatcac
    20521 gaccgataga ccactgccgc tcaagcgcgt caatatcgac aagcttatca ataatcaaga
    20581 gataattgac ttcgacgccg gctttaagca aaaatttacg aaccgccaag gccgaattct
    20641 tagaccaacg ctccaccaat aaaaagggcc agccgctatt cagcttaatt tcccgttctg
    20701 tttgtaattc taaaaaatta ttcatagaca ctaaccgacg ctatttttca tttccaattc
    20761 taccatccga ttcatttcca ctgaatattc caaaggtaat tctttagtaa tcggctcaat
    20821 gaagccattg accagcatcg ccgccgcttc cgcttccttt aagccgcggc tgcgcagata
    20881 gaataaatct tcttcattaa tcttgccggc gctggcttca tgggcgatag tggcgctggc
    20941 gttattaatt tttattaccg gaatagtgtc agaggctgac agctcgtcta aaatcaaagc
    21001 gtcacacttt accgacaccg ccgccttgtc ggcttgcgga ccgatttcca ccaacccgcg
    21061 ataaatcgct ttgccacccc ccttgctgat acttttcata acgacattgg agctggtatc
    21121 tttggcgacg tgaataactt tagcgccggt atcttgaact tgcccctcgt tggcaaaagc
    21181 aacgccgaga tgatcagcgc ttgccccctc gccgacgaga atggagcaag gataaagcat
    21241 cgtggtattg ctgccgaaat tgccgcccac ccactccata tgcgctttct tctcgacgat
    21301 ggcccttttg gtgtttaaat tataggcatc cttactccag ctttctacac tgctatagcg
    21361 gaaacgcgcg ccctctttga cataaatttc caccccgccg gcatgcaaag aatcaacacc
    21421 gtaacgagga gcgctgcagc cttcaatata atgcgcctgg gcgccgcttt cgacaataat
    21481 caaagtatgt tcaaactgcc ccatgccttt agcattcatg cggaaataag cctgcaaggg
    21541 ttcctttatc ctcaccccag ccggaatgta taaaaaagtg ccaccactcc aaaccgctcc
    21601 gtgtaaagcg gcaaatttat gcaaagtatt aggcatcgcc ttcataaaat aaggctcgac
    21661 caattccgga tgctgtttta aagcgacatc catatcttca aaaataacac cctggcgctt
    21721 gaactccgct tttaaattat gatagacaac agttgattca tactgcgcgc ccacaccggc
    21781 caagacttcc tgctctgcct gaggtatacc cagcttatca aaagtatttt taatatcagt
    21841 gggcacatct tcccagctct gcgcctgacc ttccatagcc ttggcgtaat aagtaatctt
    21901 gtccaaattc aagcgggata aatccggtcc ccaagacggc aaaggttttt cttgaaaaat
    21961 ttttaatgac tccaagcgct tcgccagcat ccaggccggt tctttttttt ccgcggaaag
    22021 acgtctcacc aaaacttcat ccaaaccgct cttggtccga gccatgtaat cagcttgatt
    22081 ataatcatcc cacaaggaat tatgtgactt taagtcttcg accataatga ttaaataaca
    22141 ctggcaaaac cttctttttc caaacgggcg gccagttccg gcccgccact ctggacaatc
    22201 ctgccactag acatcaccaa aactctatcc ggcttgatat attttaaaac acgctgataa
    22261 tgagtaaccg ccaggagact attctttttt ttcttaaaac gctgaatagc cttgcccgcc
    22321 accgccaaag cgtcaacatc gaggccggaa tcaatttcat cgaaaattgc taatatcgga
    22381 tgagcgagcg ccaattgcaa aagctcggcc tttttctttt caccgccgga aaaatcctga
    22441 ttcaattggc gattggacca atcgctcctg atatctaaag ccgccaattc atcattaagc
    22501 ttcttcttaa aagcaaaaac gctgccagct tcccgaccac gggcaacttg caaagatttg
    22561 taggcattaa ataagaaagg aaataattcc aaaccggcga tttcccgcgg ttcctgaaaa
    22621 gctaaaaaca aaccggccgc cgctctttct tcaggcttag cagagctaat gtctgtaccc
    22681 tgaaaaataa tctggccgcc agtgatttga taattaggat gacccattaa gacatgagcc
    22741 agggtgctct taccggaacc atttggaccc atgataacaa cgctctcgcc caaagtcacg
    22801 gaaaaattga tctcatgcaa aatttccttg ttaccggcgg tcacgcttaa attttttact
    22861 gtcaacaaat ccatattatt ataaaagcga agctaaattt tttttagcca agctggattt
    22921 aatatcattt aaaagcaagg gccaagcatt tttgagaccg cactgttgaa aatgcggaca
    22981 agtcttatta tgactgtgag tacagcgcac cttcatttct ccggacaagg cttccaaaat
    23041 ttctaaaatc tttatctgct tagccggccg agccaaataa taaccaccgc ccaccccttc
    23101 ccggctcttc actaaaccgg ctctctgtaa agaattggca atctggctta aatacgatga
    23161 agacacctga aggtgcttgg ccagggacga taaaggcata atctcccccg gtaatttggc
    23221 taaatgcaac ataatcaata agccataatc agttttggcg ttaatttgga acataataac
    23281 atcgtttaac tataagtaaa ttaattatag ttagtttgtt aaaaaaagca agctttaagc
    23341 cacttaaaaa aagaaagctg ccaataaccg aagttaaagg cagctggagg caagacagcc
    23401 ccaaaaacat taaaccatac aaatttacaa actaccaaac tatgaagggg ccatctggga
    23461 agagggcaag cgactaaata agatccctaa agatctctat agattggctt gcgatataaa
    23521 gtaactaacc ctattctaat acttatcctt aaaaatgtca agccgataat gtattaattt
    23581 ttttcttagc cttaacaatc gtcgctttcg cttctttctt tacttttttt acaattttct
    23641 tagctgattt gacgccggat tcaatttctt cggccgctga ttgcgtcact ttttccagct
    23701 ttttaacggc cggagcgatt tccctatcct caaaacgctt aattttaccg actgctgttt
    23761 ctaaaaaacc gtcgctctgc agaagatcaa gaatgcgatc aagcttaata tgaacagtgg
    23821 ctaattgttc ccgataatta tcacgcggcg gcatcttggt ctcttggcgc ttttgatcaa
    23881 atccgcggaa gcaggcatcg caaaaaacca gcttaccggg agtggggcga aaaggaactt
    23941 cgcaatcagc gccacaatca ctacaaaccg cttgatacat tgttggacgc tccttgtcgc
    24001 gcccgccgac accgcgctga gacttagatt taccgaaagc actgcgaccg ccaaaatctc
    24061 tttttcctcc aaatccaccc aaacgcctcg tttctcctct aaaattttgc ataaatttaa
    24121 ataatatttt ctatataata ataaataaaa tacaggcgaa tcaaagtata taaaataatc
    24181 tatttttctc gatttagcgc aaagtatagc caccatcaac aatcaaagtc tgaccggtta
    24241 tgtaatcagc taatggcgaa accaaaaaaa caactgcctg agcgatatca tccggtcgac
    24301 ccatccttcc ccaaggaatc atggcttctg tctgcttctt ggcttccggt gccatgcccg
    24361 tactggtacc tggagtctca atcacccccg gggcaacact gtttacatta attttcttct
    24421 ccgctaattc taaagccagg gccctggtta tgccaacaat accgccctta gaggcacaat
    24481 aatgagttaa accggtaaaa ccaacaaaag cggcgattga agaaatgctc acaattttac
    24541 caccggcagg cattagttta gccactgcct gagagcaaag gaaaatactc tttaaattta
    24601 catcaattac tttatcccaa tcatcctcgc ttatttccaa aaacggttta aagggataaa
    24661 tgccggcatt gttaaccaga atatctatcc gctcaaattc cgccattgtg tcagccatta
    24721 atttcgccac cgcctcgccg tcggcgacat cacacggcaa agctagggct ctaccgcctt
    24781 tatccttaat ttccgcggca gcactgacgc aatccactaa actgacatcg ctcacgacca
    24841 ccgaagcgcc ggcgctggcc aaagccagag cgatacccct gccaataccg cgaccggcac
    24901 cggtgacgat cgcaactttg ttttctaagt tcatatattt taaattttaa attaagctaa
    24961 cttatttatc caaatcaaat taattttgta aactcatcta aatatcctgc tc
//
LOCUS       DAOQSA010000015        24921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_1264_length_24921_cov_8.649562, whole genome shotgun sequence.
ACCESSION   DAOQSA010000015 DAOQSA010000000
VERSION     DAOQSA010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 24921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24921
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1264_length_24921_cov_8.649562"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2225
                     /locus_tag="PKK65_02405"
     CDS             <1..2225
                     /locus_tag="PKK65_02405"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNU81567.1"
                     /translation="MQLKIYQENAIDDLLAKSKRLLGYSGNKKVVFKSPTGSGKTIMM
                     AEFLKQLVNDREARQSLGFIWAAPRQLHIQSREKLENYFETSRALKCSYFEDLDDKKI
                     SENEILFFNWESINRANNVYIRDNEQEFNLSKVLERTKEEDREIILVIDEAHHHANSQ
                     ISQGLIQMIGPKLTIEVTATPATITDEDEKVFVQLDDVKKEGMIKKAVILNDDFDNFI
                     KQGKIKTQKLSGGSEELVIDAAMKKRQKLIKEFQKEKVNVNPLVLIQLPDRKTSLEDR
                     IRERVESILKNKYKISTEKGNNKLAIWLSGEHINKENVERQDSEVEVLLFKQAIALGW
                     DCPRAQILVLFRQWHSPIFSIQTVGRIMRMPEPDRGHYKNEILNYGYVYTNLDNIEIQ
                     EDIAKDYITIYTSKRSFDPKLSLLSCYPKRHREKTRLSPLFIEIFLKQAKEYNLKKQI
                     DTKTKKFDLKIISDYKAEDVDALTGAKIVGDKEIKASGFDMQKLFDFFVRNNLTPFYP
                     ENRSVGRVKEAIYKFFEQELKMWYGDVWEEIVQIVLSDKNSRHFVNVIDKAKEKYQTE
                     VVKRESEMVNVENWNIPESLTYSREYSVASVKKSIMQPFFSDGKWKSEVAFVNFLEKS
                     ERVEWWFKNGDRDATFFAVRYDNGEEKPFYVDFIVKLKDGRIGLFDTKAGLTKQVAGP
                     KIDGLYKYIQAENKKGKKCFGGIVANTERNYRGRWVYFNRTSKDFKDNSFENWADLVL
                     "
     gene            2376..2741
                     /locus_tag="PKK65_02410"
     CDS             2376..2741
                     /locus_tag="PKK65_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81568.1"
                     /translation="MHNRLESFSPLPYSEDSKNREKSIEEIAKFILDHLDSGPDFLSF
                     LRDQELSSPERERRWRERLEKLLSKAQKRVGYHINSRDFILAARNLILERQAAGELPP
                     KPAYLNFCELPDEDFKIDV"
     gene            2972..3622
                     /locus_tag="PKK65_02415"
     CDS             2972..3622
                     /locus_tag="PKK65_02415"
                     /inference="COORDINATES: protein motif:HMM:NF022114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HNU81569.1"
                     /translation="MEIKKTWLIIGLIIILTAIILRLGTPRNRWVCVRGQWLAQGEPH
                     GAQPQKACLDEFSVETELAELSSLLDDSFFKKEGSSPASAVVLPEESVQSDEISSSSS
                     SLEIKPEKMIELISPQPGGVMRSPYLIIGRAKGDFFVNGYLSVVLVDAQGKVIAGSLA
                     QTREDVEEGGGWVDFQAELNFSILEPIAGELLFRKDNPSGLPEQKIQVSYPVRLEP"
     gene            3627..4445
                     /locus_tag="PKK65_02420"
     CDS             3627..4445
                     /locus_tag="PKK65_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81570.1"
                     /translation="MPIEYLPNINVGSKIKLEQEKKNQFNLEKLSSFLNEQLAIKADY
                     YNKIYGQDWLDEKARLRIPETQPDKESDQLLIDIQEQTWAQESGKSVEQWRQDKEKSP
                     ASLTEAALTLMLQRVLPDNFIVVRSSVYDDYNNGVDQLIIDRESGAVICGIDEVIDRQ
                     DNVGPSKKEEKIRQKMMRGGAKVKYGARVEGKKLVLDSLKNIPAFYLSLSPKELEILA
                     ENLQENEVSVYERDLLKRLHSSLSEQLASYSHLDLNQDLKIKLEDFFKSLEKWL"
     gene            4451..5839
                     /gene="cysS"
                     /locus_tag="PKK65_02425"
     CDS             4451..5839
                     /gene="cysS"
                     /locus_tag="PKK65_02425"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNU81571.1"
                     /translation="MDLYLYNTLHNVKEKFEPIIPGKVGIYTCGPTVYNFAHLGNLRT
                     YLFEDFLVRTFAYNGYQVKRVMNITDVGHLTGDMDMGEDKMEQGAAREGRTAWEIAAY
                     YTDSFREDLQALNIVAPDIWCKATDNIAEQIALIKTLEEKGFIYRTTDGLYFDTSKFP
                     KYNCLSHLPLEELKEGARIEKNPNKKNPTDFAVWKFSPSNTKRQMEWDSPWGIGFPGW
                     HLECSAMSLKYLGKQLDIHCGGVDHINVHHTNEIAQSEAATGEKFFNYWLHGAFLNIY
                     GGKKMAKSEDNFLTLDKALLQKGISPLAYRFAALQVHYRKPMEYSEDNLRQAEQGWQS
                     LRRQLALLGDSFGKVNEAVRWEFHQAINDDLNMPQALATLFKLLKSDLPADDKRATAQ
                     DFDKVLGLDLFRPLAEKITDIPENIAALLSERQQARTNKDWSKSDVLRQAIYDAGYLV
                     EDGPEGQSLKAK"
     gene            5836..6816
                     /locus_tag="PKK65_02430"
     CDS             5836..6816
                     /locus_tag="PKK65_02430"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HNU81572.1"
                     /translation="MKKKEKNFWQDLKNQSQPILALAPMAGFTDSTFRQICSAYGADV
                     VYSEMASATALFYNQENDDNETLRLLRWDREKESHYVVQLFGSNPEHFALAARLVNEK
                     IKPHGLDINFGCPVGKVLKQGAGADLMRNLKQARAVIEAVLDNSKSPVSIKIRAKSGD
                     VSALEFMKNISDLPVSALMIHGRSLNQGFMGAVDFSLVAAIRPYFQGTILINGGVVDL
                     KTAQEALTLSGADGLALARGALARPWLFQEIKEGREIIYDRSAISRLLYRHALSLVER
                     RGESALVEWRKQACWYIQGLAGASHWRRRLVQVSTLTNLENILKDYGLND"
     gene            6800..7174
                     /gene="tsaB"
                     /locus_tag="PKK65_02435"
     CDS             6800..7174
                     /gene="tsaB"
                     /locus_tag="PKK65_02435"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:NF013012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNU81573.1"
                     /translation="MVLTINTSLGDEIAVSLLTGNKTINSKIKAPRQQSEKLLPLIEK
                     LLFKHHLSWRSIKEIRVQNEGGSFTSLRIGVLTANALAYALGIPVTPLNGGKSFKFVG
                     GQAVRPKYQSEPNIGKKVMLAC"
     gene            7369..7800
                     /gene="mraZ"
                     /locus_tag="PKK65_02440"
     CDS             7369..7800
                     /gene="mraZ"
                     /locus_tag="PKK65_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNU81574.1"
                     /translation="MFIGEYNHNLDDKGRLAIPAKFRAILKKGAVVTKGLDNCLFLYS
                     KEQFEKIAQKFASLPISQAKARAFSRHMLAGAMEVDFDNQGRITLPEYLRKFSALKKG
                     VIIAGLYNHLEIWDETAWRRYKQTAEQNSNSIAEELGDLGI"
     gene            7802..8743
                     /gene="rsmH"
                     /locus_tag="PKK65_02445"
     CDS             7802..8743
                     /gene="rsmH"
                     /locus_tag="PKK65_02445"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNU81575.1"
                     /translation="MTYRHQPVMLSEVLSVLSPRPGQIYIDCTLGGAGYTLALAAAVG
                     KKGKVLAIDADPLAIANAERIIKEKKIENIVLVHDNFKNLAAISNAYLKSSADGIVFD
                     LGLSSAQLDDENRGFSFQGDRPLDMAFGAGADIETAALLNRASLSELTRIFRELGEEP
                     QAYRLAKEIVIARQNKSFTSTKPLVDIILKVTPPRLRSGKIHPATRVFQALRLATNDE
                     FGSLRSALTAVPTVLKKGGHLAVVSFHSGEDRIVKQWLKEESTDCLCPPRAPVCVCGH
                     KASFLSLLKKPLLPSAEEINFNPRARSAKLRAAEKIR"
     gene            8798..9133
                     /locus_tag="PKK65_02450"
     CDS             8798..9133
                     /locus_tag="PKK65_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81576.1"
                     /translation="MIIRSVNNPKRQQTAKKNFSWRTLNIFLSIACVVLGVVYLVGIN
                     DLTVKGFVLKDLKNQQQMLMEANQDLQTKVLGLQSYASISPRLQGLNMVAVEDVVYLS
                     SKAPAVAKK"
     gene            9148..11223
                     /locus_tag="PKK65_02455"
     CDS             9148..11223
                     /locus_tag="PKK65_02455"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNU81577.1"
                     /translation="MWRDYRQKSPTISSINPRLRLFAALIFLLLAAIVYRLYFLQVSQ
                     GDWYAALASGQHQISSLLQPVRGKIMLHEKLNGQDQLYPLATNKDFSFLYAVPKDIKN
                     PQDLADKLFDFFDKPELEAKAKRELEAELKNNLQKEIDFINASEYSAEDKAARIKAAQ
                     DLALAKKDKADWQEMQEILLERLIKEKREEAIAAYLKKLDKPGDPYEPLREKLDDNTL
                     LSLYAFLSSSTERPVAASDLERRLDKVVFKESGEELKIEGIAFNLQSYRFYPENNMAS
                     HIVGFVGMANDKVSGRYGLEEFFNDELAGRAGYLKGERGVSNTIIVNDREYTRPEDGS
                     DLILTMDRSVQFQACSYLEEAVAKFKAEGGSVIAVNAKTGAILAMCSVPNFNPNNYKD
                     VSDIKVYNNPAIIYQYEPGSVFKVITMAAAIDQGKVKPSTTYKDEGQIMIKGWPKPIS
                     NSDFSTKGAHGVVDMNAVLENSLNTGAIFAMRQIGAKTFADYVKNFGFGEKSGIELGS
                     ESAGNIDNLLKNNIREIDAATASFGQGIAVTPLQMLMSYQALANQGELMKPYIVQEIR
                     HSNGQVDEFKPHPLRQVVSEQTANTILAMLVNVVESGHAKGAKIPGYYIGGKTGTAQV
                     ATSGGYSADKFIHTFIGIVPIDNPQIVMLTKIDAPKGAAYAESTAVPLWHDIADFLLK
                     YYQIPKTRK"
     gene            11242..12528
                     /gene="murF"
                     /locus_tag="PKK65_02460"
     CDS             11242..12528
                     /gene="murF"
                     /locus_tag="PKK65_02460"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNU81578.1"
                     /translation="MKKLLQLKLRFLSRLILKKYRPEIVAISGSIGKTSAKEAIFQVL
                     SGRLAVRTSIKNYNNEIGVPLTIIGAESPGSSFVGWLRVFGRAWLLYFRRDLSYPKIL
                     ILEMGIDRPGDMDYLTRLAPPTVGVITAVSYSHLEYFGSFQNIKKEKQILIERVARKG
                     LSILNYDNEAARSMAEVSPARVLSFGLLPGADIVAQDLHYRYQGASYDIAGLNFKLNY
                     EGNIVPVEMPNIIGEGGVYAALAATAVALHFGLNLMEIASALRNLRLPAGRMQLLPGI
                     KHTFLIDDSYNSSPEACLSALKVLGQIKIDGAASKYAVLGDMLEIGAYSEEGHALVGR
                     TLFEQGINYLVSVGERSRDIDRGAKEAGMSSDFIFHFDDSATAGRFVQERLHEGDVVL
                     IKGSQGTRMERVTKELMAEPDKAEELLVRQGPGWQD"
     gene            12600..13475
                     /locus_tag="PKK65_02465"
     CDS             12600..13475
                     /locus_tag="PKK65_02465"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNU81579.1"
                     /translation="MSILILGAAGNLGGQLKKVFSNQDCQAWDRLDFDFLNFEELSKR
                     LHTLSPSVIINAAAYNAVDKCEENEAEAMLARRLNTELPGVLSDYCLSSQTLLIHYST
                     DYVFGGDGRLSGPYQETDKPSPLNVYGRTKFAGEQEIARRALNGLDYYLIRTSKLFGP
                     KGESPQVKPSFFDIMITLSLEKETIKAIDGERSCFTYTPDLAIATKSLVTDRPARGIY
                     HLCNSGEATWYDGANYLFQAIGATVNLQALASADWPRPAVRPQYSALANHRRPSLRPW
                     TEALDEYLFYLGLKK"
     gene            13493..14218
                     /locus_tag="PKK65_02470"
     CDS             13493..14218
                     /locus_tag="PKK65_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012036820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNU81580.1"
                     /translation="MRGIILSGGSGTRLRPLTKITSKQLLPIYNRPMIYYPLDTLIKA
                     GIKEILIIVSPERAGDYLNLLGSGREFGVKFTYEIQDKPEGLAQAFIIGEKFIQGENV
                     VMILGDNIFEDNFADDISNFKSGAKVFAKYVPDPERFGVVKFDDKMKAEKIIEKPTNY
                     LSNYAVTGLYVYDHRVVEVAKNLKPSARGELEITDLNNWYLAKGELEVAMVEGEWLDA
                     GTFDSLLKAQLFAKEKLDAKMSI"
     gene            14243..15211
                     /locus_tag="PKK65_02475"
     CDS             14243..15211
                     /locus_tag="PKK65_02475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNU81581.1"
                     /translation="MKLAVAFLCYNDSSAPYLPEFLKSLEAALIRLDAEIFVFAGDNS
                     DRAPFVNQELLFRHNLKASFPIKLLAFDKNLGFAAAYNRLIIAAKGVGADYFLMINPD
                     MLLTENMIARLLSVLAKRTDLAAVCPKIYRWDFRGKRLTKIIDSCGIGLAPGLRFYDL
                     GQGKEDKGQYDRTEIIGPSGAAALFRISSLEKVTVDGKYFDERFFMYKEDCDLAYRLF
                     RAGQKTVLVPDAIAYHDRSSAGQENLWQTWRHWQKRSRLTRSWSFANQNLIFVKYFHF
                     ESYFSRFLIVVKVIFMGLFSLIFAPFLLKTYFKFWGVNRSINRSID"
     gene            15289..16332
                     /locus_tag="PKK65_02480"
     CDS             15289..16332
                     /locus_tag="PKK65_02480"
                     /inference="COORDINATES: protein motif:HMM:NF016432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HNU81582.1"
                     /translation="MDNFLESIVRDKRSEELSKLNAIQIVNSLFSDLMDRERDILTRR
                     FGLNGDKNETLEKIGQLHKLTRERVRQIENASIKKIKRLDNLSNYLVTLKSVVEELLK
                     EHGGLLRRDFLLDILTVMSLEMNNDVDINSAEYEKNRQVYKNRFNFILSKLLDDDFDL
                     MPDSSNFNASVKLKEESLEHFEKLSKDLLSKLESLNKTFDTEGVLSLLKSLDSYSQYQ
                     DKLEANNSADITPIFKSQIFPDKAEIINSNKTLYSLMQALKDLERNKFGYWGKADWQE
                     IKPKTINDKIYLVLKNAGKPLHFTEIASKINEIKFDHKNANPATVHNELILDERYVLT
                     GRGMYGLREWQNA"
     gene            16474..17913
                     /locus_tag="PKK65_02485"
     CDS             16474..17913
                     /locus_tag="PKK65_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81583.1"
                     /translation="MEITINTSRLDAFFALPADQMLWVFFINIGWMVIAAVLLYGISL
                     IYLQKIRSQWAAKNLQFILLAIDIPKGNEQSPKAAENMFSYLAGAHGSTNFFEKWFEG
                     KFQVSFSYEIVSIEGYTQFLIRTPVEFRDLIETSVYSQYPDAEISEVDDYVDTVPHHF
                     PDEDYDVWGAEFIQSNNWAFPIKLYQEFEHNFGESETQFKDPMASLMDLCSSLREGEQ
                     LWMQLVIIPIGFDWVAETDKVVDKILGKKKKQSSAASKFVEWLGNISEAIYSIWGDIE
                     SKSTEDKGLSMQELTPKQKRQIEAIHLKAAKLSFEAKIRVVYVARKDLMNKNKVVNGV
                     IGYMKQFASLDLNNLKPDVKLTMTKTAYFNKDKRLLRKKNAIMANYIAREDTAGRNPG
                     LYNIEELATLWHFPIEANVKAPLIQKAPGRKADAPSTLPIVNDMAAPLLEPFSVEILH
                     SATSQSQSESKNHIKEEEKQNVPDNLPFV"
     gene            17913..19103
                     /locus_tag="PKK65_02490"
     CDS             17913..19103
                     /locus_tag="PKK65_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNU81584.1"
                     /translation="MFNKKTKKRIGIDARFYGPLGKGLGRYVQEVVDNIVSMTVTSSE
                     FEYVIFLSPDNFKDFKSSHPNVRKIEVPWRWYSWREQLFFPLILKKEKLDLVHFPHFN
                     VPILTPVPFVVTIHDLILTRFSSRRASMLPAAFYWFKQAAYRLVIRTAIRRARAIITV
                     SEFTRRDIIKKFKANPKKLFVTYEGVADLDKKEIIGEIEEKQVLQRYRISEPFLLYVG
                     NAYPHKNLEVLLTVFNNLLSRHHHLKLVMVGKDDYFYHRVKEKASALSLWQSEGSDNN
                     KVIFPGYVPDADLAVLYRRALFYVFPSLYEGFGLPPLEAMSHSCPVVSSNQASLPEVI
                     GDAALYFDPYNQADMLSKLEFLFENEDYRQQLIARGLKRVNVFSWQKCARQTLEIYRK
                     ILVE"
     gene            19115..21601
                     /locus_tag="PKK65_02495"
     CDS             19115..21601
                     /locus_tag="PKK65_02495"
                     /inference="COORDINATES: protein motif:HMM:NF024594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HNU81585.1"
                     /translation="MNRENIKKSNIKRNLSAVDSFIVDLRPSGQKKLSESQKPVNIAF
                     INWQKFKRIFSFRRPPYAATLSFFKSNSNLVSPSKNSSFSLRHFFNFQAKKRRHFRIS
                     RSHYLRGSRFNKRERRLFAAAAGVRWRRLKKEKSKTNARLSQAEHLVSWYRSLFVFVS
                     VLIIIILPFKILSYLKILDIKSLQAGVIDRSLSALNNLAAASDGAAQLDFSAAASNFA
                     KAESDFISAQAELSRIDDWLLSFASFSGDPKIKLASSGNKFLAAGQAAAAMGEHLSEA
                     GNILLQTSADKNWGSLIDSFIEPGQQALIDAEELQRQLDNIDVDTLPEEYRSQFIDFR
                     DQAEIAVSSLRSLLGSAQEIKDFLGVSRDKRYLLVFQNNAEMRGSGGFLGSYALVDIR
                     EGRIRRLEVPAGGSYDTEAGMKNFIQSPQPLWLVSPRWYFWDANWWPDWPTSARSLMW
                     FYEKSGGPTVDGVISFTPDVLSDLLKITGPIDMQAEYGLTIDADNFWDLIQATVEKEN
                     LIKTHPEEVANLPDSPKNQPKKIIGDLMAEIIDRLPRILTFENLPLLITALEKNLSSK
                     NIMLYFSDPLLQAKAARYGFDGAMLPSQHDYLLIAHTNIAGQKSDRKMLDEVKHSVQI
                     LSDRSLIDTLTITRTHTGLKNEILSGVRNVDWLRVYVPAGSQLLEARGFRAPDASYFE
                     KPEEGWEKFPLIAETEELALTDLVSGTKIYKENGKTVFANWVMVDPGETVEIKLRYRL
                     PFRLEAEADVSGGDWLTRLGSLLDKAPGERFPFSLLLQKQPGAKATSVDLEITAPSGW
                     RQIWNYPNQSSWNGNLSLDRDVMRAVLLEK"
     gene            21691..23694
                     /locus_tag="PKK65_02500"
     CDS             21691..23694
                     /locus_tag="PKK65_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81586.1"
                     /translation="MTVLKKKKKTSSADKDSGLKTFLERPLPAVEEVSRFEEAVKKEA
                     AAEEVENNLSAIYHDKQGRLVDVSRVQKRHRLRLLVLFKQVFILTLLACGLYAGYYYY
                     FQRPAGTSAIILTVQAPEKALAGEPISYNITYSNDSGLALSNAKLEVLLPSNFIITES
                     VPAPSGINTWSFGDIAIGGSGLVVISGYLNSPVDSANVVSARLSYVPANFSSEFKKEA
                     SANTVVSGLGFIVSADYLNTAIIGQNNEMKLFLNAFKNNKLSDLYLKISGSDNFIVNQ
                     VSSEKGELVSENNQSEGAAADVFKIQNTGENSWFLSGLPADSEEKITIAIDFTLKTKN
                     QDKEDLSLRLIKREADGREMSLWEKTISFEAIKSDLNLSLTLNGSKTDQTLNFGDTLS
                     YQLEYANNGEATLDDVALMAVIKGDFVDWSSLRDPLGGSVAGDAIVWTKEQLPSLSEL
                     TPGASGKIDFSLKLRNFSVSDLGVDTSITSYAQYGLNNNQEGASEDNRSNTIKGQINS
                     DLSLSEKILYFNEDNIPVGSGPLPPRVGEKTSVRVFWTLKNNLHDLSDVQVVLDLPQG
                     LEWSGGENTNVGSITYDAESNQVTWRLGFLPISVYRADAEFNISFIPKDSDRDKILVI
                     SPGSTVTALDSLTGGRISYKTKAKTSKLEDDEIAGFSNNGRVE"
     gene            23697..24851
                     /gene="tgt"
                     /locus_tag="PKK65_02505"
     CDS             23697..24851
                     /gene="tgt"
                     /locus_tag="PKK65_02505"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNU81587.1"
                     /translation="MSGFKLKLFKDGLRRGRVTNIHGSLSTPFFMPDATRAFVKANTS
                     ADAKRAGTEVMVVNTFHLYLQPGQKIIKRAGGVHNFMDWQGPLLSDSGGFQVFSLIHQ
                     GKNLGKINDDKVIFKSPIDGSRHEFSPEKSIQIQFDLGVDMMVCLDDCPPNDWPRADL
                     EKAVKRSLAWAARCKQEYLKQIKRRKITEDNRPLLFAVVQGGDYEDLRRESARQLLAI
                     GFDGYGFGARPVDKDGNFLAETIFKTASFLPKDSLRFALGVGMPEDIYRCYLMGWDMF
                     DCVIPTREGRHGKLFFFKPGFRGFTKRKTKPGPVVSYPDFYRTKNINNAAFAQDFSPI
                     NADSDLPELRRYSKAFLHHLFRLDEPLGQKLASLNNLEFYNRLLRELRRL"
BASE COUNT         7218 a         4330 c         5690 g         7683 t
ORIGIN      
        1 ctatgcaact aaaaatttac caagaaaacg caattgatga tttattggcc aagagcaaaa
       61 gacttttggg ttattctggt aataaaaaag tagttttcaa atcaccgacc ggttcgggca
      121 aaacaataat gatggcggaa tttttgaaac aattggttaa cgatagagaa gccagacaat
      181 cgctcggttt tatttgggcg gctccaagac aacttcacat tcaaagccga gaaaagttag
      241 aaaactattt tgagacaagc cgagcgctaa agtgttccta ttttgaagat ttggacgaca
      301 aaaaaatcag cgaaaacgag attttgtttt ttaactggga aagtataaat agggcaaata
      361 atgtttatat tcgcgataac gaacaggagt ttaacttgtc aaaggtatta gaaagaacaa
      421 aagaagaaga tcgagaaata attttggtta ttgatgaagc ccaccaccac gccaacagcc
      481 aaatttcaca aggtttaatc caaatgattg gacctaaatt gacgattgag gttacagcaa
      541 cgccagcaac tattactgat gaagatgaaa aggtgtttgt tcagttggat gatgttaaaa
      601 aggaaggaat gattaaaaag gcagttattt taaacgatga ctttgataat tttattaaac
      661 aaggtaaaat caaaacgcaa aaattaagcg gcggaagcga agaattggtt atagatgcgg
      721 cgatgaaaaa gagacagaaa ttaataaagg aatttcaaaa agaaaaagtt aatgtaaatc
      781 ctttagtttt aatccaattg ccggatagga aaacgagcct agaagataga attagggaaa
      841 gagtagaaag cattttaaaa aataaatata aaatttcaac agaaaaaggc aataataaac
      901 tagccatttg gctttcgggc gaacatatta acaaagaaaa tgttgagagg caggacagcg
      961 aggttgaggt tttgcttttc aagcaagcaa tcgctttagg ttgggactgc ccaagagcgc
     1021 agattttagt tttgtttagg cagtggcaca gcccgatttt ttccattcaa accgttggca
     1081 gaattatgcg aatgccagag ccagatagag gacattataa aaatgaaatt ttgaattacg
     1141 gctatgttta tactaatctg gataatatag aaattcagga agatattgct aaagattaca
     1201 tcactattta tacaagcaag aggagctttg accctaaatt aagtttgctt tcgtgttatc
     1261 ccaaaagaca tcgcgagaaa acaagattgt cgccgctttt tatagagata tttttgaaac
     1321 aagccaaaga atataattta aaaaaacaga ttgatactaa aaccaaaaaa tttgacctaa
     1381 aaattatctc tgattataaa gccgaagatg ttgatgcttt gactggggca aaaattgttg
     1441 gcgacaaaga gataaaagca agcggttttg atatgcaaaa attgtttgac ttttttgtgc
     1501 gtaataattt aacccccttc tatccagaaa atagatcggt tggcagagtt aaagaagcta
     1561 tctataaatt ttttgagcag gaattaaaaa tgtggtacgg cgatgtgtgg gaagaaattg
     1621 ttcagattgt tttgagtgat aaaaattctc ggcattttgt taatgttata gataaagcaa
     1681 aagaaaaata tcaaacagaa gtggtaaaaa gggaaagtga aatggttaat gtggaaaatt
     1741 ggaatattcc agaatcttta acttacagca gagaatattc agtagcgagc gttaaaaaat
     1801 caattatgca gccatttttc agcgacggga aatggaaatc ggaggtggct tttgttaatt
     1861 tcttggaaaa atcggaaagg gttgaatggt ggttcaaaaa cggcgaccga gacgcaacat
     1921 tttttgccgt tcgctatgat aacggagagg agaaaccttt ttatgttgat tttatcgtga
     1981 agttgaaaga cggccgaatt ggactttttg acacgaaagc tggattgaca aaacaggttg
     2041 ccgggccaaa aattgatgga ttgtataaat atatccaagc cgagaataaa aaaggcaaaa
     2101 aatgttttgg cggaatagta gctaacaccg aacgaaatta tcgcggacgc tgggtttatt
     2161 ttaacagaac aagtaaagat tttaaggata actcttttga aaattgggct gatttggtat
     2221 tataaaaaaa taccagtctg ccgaaggcgg acaatggggt aggaagcatt tccgcaattc
     2281 tgcgttagca gaatatcaaa atgttgggtc gggattcaaa aatccgcaag cggatttttc
     2341 tcaaaatgag ttcgagtcca ccaacgacca ctactatgca caatagactg gaaagttttt
     2401 ctcctcttcc ttatagcgaa gattcaaaaa atcgcgaaaa aagcattgag gaaattgcta
     2461 aatttattct tgatcattta gattccggtc cggattttct atcttttttg cgcgaccaag
     2521 aattatcttc tccggaaaga gaaagacgtt ggcgcgaacg tcttgaaaaa cttttatcaa
     2581 aagcgcagaa gcgagtcggc tatcatatta atagccgcga ctttattttg gcggcgcgca
     2641 atcttatttt agagcgtcaa gcggccggag aattgccgcc caagccggct tatttaaatt
     2701 tttgcgaatt gccggatgaa gattttaaga ttgacgttta gtcgcagtta atcttcaaag
     2761 ttctgataga gctttttgtg taaaaaaaag gggataccga aagtatcccc cgtggactgt
     2821 tagttaagag taaagcagaa aaacttctta aaacagccct aagacaaaga gctattttat
     2881 aatagtattt attttataaa ttgtcaaatt ttagtgattt tagatagatt ttttggggat
     2941 acatgttaaa ctacaagagt tagatgacat tatggaaatt aagaaaactt ggctaattat
     3001 tggccttatt attatcctta ccgctattat cttgcgcctt ggcactcctc gcaatcgttg
     3061 ggtgtgcgta cgcggacaat ggttggcaca aggagagccg catggcgctc agcctcagaa
     3121 ggcgtgtttg gatgaatttt cagtggaaac agagttagcc gagctctctt ctttacttga
     3181 tgattctttt tttaaaaagg aaggctcatc cccagcgtcc gcggttgttt tgccggaaga
     3241 aagtgtgcaa tcagatgaaa tatcatcatc atcatcatcc ctagaaataa aaccagaaaa
     3301 gatgatagaa ttgatttccc ctcaaccggg aggggtaatg cgctccccct atttaattat
     3361 aggcagagcc aagggcgatt tttttgttaa tggctattta tctgttgttt tagttgatgc
     3421 ccaaggcaag gttatcgctg gcagtctggc tcaaacccgg gaagacgtag aggaaggagg
     3481 gggttgggtt gattttcagg cagaattgaa tttttcaatt ttagagccga ttgcggggga
     3541 actattattt aggaaagata atccttccgg tttgccggag cagaagatac aagtctctta
     3601 tccggtaagg ctggagccat aattatatgc cgattgaata tttgcccaat attaatgtgg
     3661 gcagcaagat taaattagaa caagagaaaa aaaaccagtt taatctggaa aaattatcat
     3721 ctttcttgaa tgaacagtta gcgattaaag ctgactatta caataaaatt tatggtcaag
     3781 attggctgga tgaaaaagcg cgtttgcgta tacccgaaac acaaccagat aaagaatcgg
     3841 accaattatt aatagatatt caagagcaaa cctgggcgca ggaaagtggc aaaagtgtag
     3901 agcaatggcg ccaagataaa gagaaaagtc cggcatcatt gactgaagcg gctttaacct
     3961 taatgttgca gagagttttg cctgataatt ttatcgtggt gcgttcttcc gtttatgatg
     4021 attataataa tggcgttgat caattaataa tcgaccgcga gagcggagcg gttatttgtg
     4081 gtatcgacga agttatcgat cgtcaagata atgttggtcc gagtaagaaa gaagaaaaga
     4141 ttaggcaaaa aatgatgcgc ggcggcgcca aagtaaaata tggggctcgg gtagagggaa
     4201 aaaaattagt attggacagt ctcaagaata tccccgcttt ctatttgtct ttaagcccga
     4261 aagaattaga aattcttgcc gaaaacctac aggaaaatga agtttctgtc tatgagcgcg
     4321 atttgctaaa acgtcttcat tcttctttat ctgagcaatt agctagctat tctcatcttg
     4381 atttgaatca agatttaaaa ataaaacttg aagatttttt taaatcttta gaaaaatggc
     4441 tttaattttt atggatttat atctttacaa tactttacat aacgtcaagg agaagttcga
     4501 gccgataatt cccggcaagg tgggaattta tacatgcggt ccgactgtct ataattttgc
     4561 ccatctcggt aatttacgca cctatttatt tgaagatttt ttggtccgca cctttgccta
     4621 taacggttat caggtaaagc gggtgatgaa tattaccgat gtcggtcatt taactgggga
     4681 catggacatg ggcgaggata agatggagca aggggcggct agggaaggtc ggactgcctg
     4741 ggaaatcgct gcttattata ccgattcttt tcgcgaagac ttacaggctt taaatattgt
     4801 ggctccggat atttggtgta aggctaccga taatattgcc gagcagattg ctttaattaa
     4861 gactttggaa gaaaagggtt ttatttatcg cactacggac ggtctttatt ttgatacctc
     4921 caaatttccc aagtataatt gtttaagcca cttaccctta gaggaattaa aagaaggcgc
     4981 gagaatcgaa aaaaatccca acaagaaaaa tccgactgat tttgcagtct ggaaattttc
     5041 tccatccaat acaaagcgcc agatggagtg ggattctcct tggggcatag gttttcctgg
     5101 ttggcactta gaatgttcgg cgatgagcct taaatattta ggtaagcagc tggatatcca
     5161 ttgtggcggc gttgaccata ttaatgttca tcataccaat gaaattgctc aaagtgaggc
     5221 ggcgaccggt gaaaagtttt ttaattattg gcttcatggt gctttcctta atatttacgg
     5281 gggtaaaaaa atggcgaaga gtgaagataa ttttttaacg ttagataagg ctttattaca
     5341 aaaaggcatt agtcctctag cctatcgttt tgccgctctc caggtgcatt accgcaagcc
     5401 aatggaatat agtgaagata atttacgcca agcggagcaa ggctggcagt ctttacgtcg
     5461 tcagcttgct ttacttggtg atagtttcgg caaggttaat gaagctgttc gctgggaatt
     5521 tcatcaagct attaatgatg atttgaatat gcctcaagct ctcgccaccc tatttaagct
     5581 gttaaagtct gatttaccag ctgatgataa gagggcgact gctcaagatt ttgataaggt
     5641 tcttggtctt gatttattcc gtcccttggc ggaaaaaatt acagatatcc cagaaaatat
     5701 tgccgccctg ttatccgaac gtcagcaagc gagaaccaat aaagattggt caaagtctga
     5761 tgttttacgt caagcgattt atgatgccgg ctatttagtc gaagacggcc cagagggaca
     5821 atctttaaaa gccaaatgaa gaagaaggaa aaaaattttt ggcaggattt aaaaaaccag
     5881 agtcagccga ttttggcttt ggcgccgatg gccggtttta cggatagcac ttttcggcag
     5941 atatgttcgg cctacggggc ggatgtggtg tatagcgaaa tggcgtctgc caccgctttg
     6001 ttttataatc aggaaaatga tgataatgaa actttgcgtt tattgcgttg ggatagggag
     6061 aaagaaagtc attatgttgt tcagcttttc ggttcaaatc cagaacattt cgccctggcg
     6121 gcgcgtttag tcaatgaaaa aataaagcct cacggtctag acattaattt tggctgtccg
     6181 gtaggcaagg ttttaaaaca gggagcaggg gcggatttga tgagaaattt aaagcaagcc
     6241 agggcggtga ttgaggcggt tttggataat tctaaatcac cggtatcaat caaaataaga
     6301 gctaagtcag gagatgtttc tgcacttgag tttatgaaga atataagcga tttgccggtt
     6361 tcagccctaa tgattcatgg tcgtagctta aatcaaggat ttatgggggc agtcgatttt
     6421 agtttagtgg cggcgattcg tccttatttt caaggaacta ttttaattaa cgggggagtg
     6481 gtcgatttaa aaaccgcgca agaggccctg actctcagcg gggcggatgg tctggctttg
     6541 gctcgaggcg ctctcgcccg tccttggtta tttcaagaaa taaaagaagg cagagaaatt
     6601 atatatgatc gcagtgctat tagtcgcctg ctttatcgtc atgccctcag tttagttgag
     6661 cgaagaggag aatcggcctt agttgaatgg cgcaagcaag cctgttggta tattcaagga
     6721 ttagccggcg ctagccattg gcgtcgccgc ttggtgcaag tttctacttt aacaaattta
     6781 gaaaatattt taaaagatta tggtcttaac gattaatact agtttaggcg acgagattgc
     6841 ggtttcttta ttaacgggaa acaaaacaat taactctaaa attaaagcgc caagacagca
     6901 gtcagaaaaa cttttaccct taattgaaaa gcttttattt aaacatcatc tctcttggcg
     6961 gtcgattaaa gagattcgcg ttcaaaacga aggcggtagc tttacttctt tgcgtattgg
     7021 cgttttaacc gctaatgctt tagcttacgc tttgggaata ccggttactc ctcttaatgg
     7081 cggtaaatct tttaagtttg tcggtgggca ggcggtaaga cctaaatatc agagtgagcc
     7141 taatatcggt aagaaagtga tgttagcctg ttgataactt ttttattttt ttggcagctt
     7201 gtaaagcggc ttgtggaaaa agccgtcttt tttttatttt tccctttatt tttgtggttt
     7261 ttgggggttg accttagggg gttagtggtg tataattaaa tcattaaggt gaaaagtggg
     7321 gaagagaggt gaaaaccttt atagacactt tatccaccgc cacgtcctat gtttatcggc
     7381 gaatacaatc ataacttgga tgataagggg cgcttagcta ttcccgctaa gtttcgtgcc
     7441 atcttaaaaa agggagcagt cgttaccaag ggtttggata actgcttatt tttgtattca
     7501 aaggagcagt ttgaaaaaat tgcccagaag ttcgcttcat tgcccattag ccaagctaag
     7561 gctagggcct tttcccgtca tatgttagcc ggtgccatgg aggttgattt tgataaccaa
     7621 ggacgcatta ctttgccaga gtatttgcgt aaattttctg ctttaaaaaa aggcgttatt
     7681 atcgccggct tatataatca tttggaaatt tgggatgaga ccgcttggcg tcgctacaag
     7741 cagacagctg aacagaatag taatagtatt gctgaagaat taggagattt aggtatttag
     7801 tatgacgtat cgtcaccagc ctgtaatgct gtcagaggtt ctgtctgttt tatcgcctcg
     7861 tcccgggcag atttacatcg attgtacttt gggtggtgct ggctatactt tagccttggc
     7921 cgccgccgtt ggcaaaaagg gtaaggtgtt ggctattgat gctgatcctt tagctatcgc
     7981 taacgccgaa cgtatcatta aagaaaaaaa gatagaaaac atcgttttag ttcatgataa
     8041 ttttaaaaat ttagccgcta tcagtaatgc ttatctcaag tcttctgccg acggcatcgt
     8101 ctttgatctt ggcttatctt ccgcccagct agatgatgag aatcgcggct tttctttcca
     8161 gggagaccgt cctcttgaca tggcttttgg cgctggcgcc gatattgaaa ctgcggcttt
     8221 actcaaccgc gcgtccttat cggagttaac tcgtattttt agagaattag gggaagaacc
     8281 gcaggcatat cgtttagcta aagaaatagt aattgctcgt caaaataaat cttttaccag
     8341 tactaagcct ttggtcgata ttattttaaa ggttactccg ccgagattgc gctccggcaa
     8401 gattcatccg gctacacgcg ttttccaagc tttgcgctta gcgaccaatg atgaatttgg
     8461 cagtttgcgt tcagctttaa cggcggtgcc gacggtgtta aaaaaggggg gacatttggc
     8521 ggtggtttct ttccatagcg gtgaagacag gatagtgaaa caatggttaa aagaggaaag
     8581 tactgattgt ctttgtccgc cgcgcgctcc cgtttgtgtt tgcggacaca aagcttcttt
     8641 tctctcttta ttaaagaaac cgttattgcc cagtgccgag gaaattaatt ttaatcccag
     8701 ggcgcggagt gccaaattga gggcggcaga aaaaatacga taattttcaa atttatatat
     8761 ttatagcctt tggctttaat tttaagcaac tatcagtatg attattcgct cagttaataa
     8821 tccaaaacgc cagcagacag ccaagaaaaa tttttcttgg cggacgctta atattttttt
     8881 aagtatcgct tgtgtcgtct tgggggtggt ttatttagtc ggcattaatg atttgacggt
     8941 taaaggcttc gttcttaagg atttaaaaaa ccaacaacag atgttgatgg aggctaatca
     9001 ggatttgcag acaaaagttt taggattaca atcatatgct tccatctctc cccgcctgca
     9061 aggattgaat atggtggcgg ttgaagatgt ggtctatctt tcctcaaaag cgccagcggt
     9121 agctaaaaaa taattctcat ctttcttatg tggagggatt atcgtcagaa aagcccgact
     9181 ataagtagta ttaatccgcg tttgcgcctc tttgcggcgc ttattttttt attgttggca
     9241 gcgattgttt atcgtttata ttttttacaa gtttctcagg gcgactggta tgccgcgctg
     9301 gcgtccggac agcatcagat ttccagctta cttcagccgg taagagggaa aattatgctt
     9361 catgaaaaat taaacgggca ggatcagcta tatcctttgg cgaccaataa agatttttct
     9421 tttctttatg ccgtgccgaa agatattaaa aatcctcagg atttagcgga taagctgttc
     9481 gatttttttg ataaaccaga attggaagcc aaggctaaga gggaactaga agcggagtta
     9541 aaaaataatt tacaaaaaga aatagatttt attaatgctt ccgaatatag cgctgaagat
     9601 aaagctgcta gaattaaggc cgctcaagat ttagcgttgg ctaaaaaaga taaggccgat
     9661 tggcaggaga tgcaggaaat acttttagaa agattaatta aagagaagcg ggaagaagct
     9721 atcgctgctt atttaaaaaa actcgataag ccgggtgatc cttatgagcc tttgcgcgag
     9781 aaattagatg acaacacact tttgtcttta tatgcttttt taagcagttc gacagagcgc
     9841 ccggtagcgg cttctgattt ggagaggcgg ctggataaag ttgtattcaa agagagcggg
     9901 gaggagttaa aaatagaagg tatcgcgttt aatctgcaga gctatcgttt ctatccagaa
     9961 aataatatgg cgtcccatat cgtcggtttt gtcggtatgg ctaatgataa ggtctctggt
    10021 cgttatggct tggaagagtt ttttaatgac gaattggcag gcagagctgg ctatcttaaa
    10081 ggggagcgcg gtgtcagtaa tactattatt gttaatgatc gcgaatatac tcgaccggaa
    10141 gatggcagtg atttaatttt aaccatggat agaagcgttc aatttcaggc ttgttcttat
    10201 ttagaggagg ccgtagctaa atttaaagct gaaggcggca gtgtgattgc tgttaatgct
    10261 aagactggcg ctattttggc gatgtgctcc gtgcccaatt ttaacccaaa caattataaa
    10321 gacgtcagcg atattaaggt ttacaacaat ccggctatta tttatcaata tgagcctggc
    10381 tccgttttta aagtcatcac tatggccgcc gccattgatc aaggcaaggt taagcccagt
    10441 accacctata aggatgaagg acaaattatg attaagggtt ggccaaaacc gattagtaat
    10501 tctgatttta gcacgaaagg cgctcatggc gttgttgata tgaatgcggt tttagaaaac
    10561 tctttaaaca caggggctat ttttgcgatg cgtcagattg gagctaagac ttttgctgat
    10621 tatgtcaaaa atttcggttt cggcgagaaa agcggcattg aacttggttc cgaaagcgct
    10681 ggcaatattg ataatctact gaaaaataac attagggaaa tagacgcggc taccgcttct
    10741 ttcggtcagg gtattgccgt aaccccttta cagatgctca tgtcctatca agctttagct
    10801 aatcagggag aattaatgaa gccttatata gttcaagaaa taaggcatag caacggacaa
    10861 gtcgatgaat ttaaaccgca tcctcttcgt caagtggttt ccgaacagac cgctaatact
    10921 attttggcga tgttggtcaa tgttgttgaa agtgggcatg ctaaaggggc gaaaattcct
    10981 ggttactata ttggcggaaa aaccggtacc gctcaggttg ccacttccgg aggttatagt
    11041 gcggataaat ttattcatac ctttatcggt atcgtgccga tagataatcc tcaaattgtc
    11101 atgctcacga agattgacgc gcccaagggg gcggcttatg cggagagtac cgctgtgcct
    11161 ctgtggcatg atattgccga ttttcttttg aagtattatc aaataccgaa gacgagaaag
    11221 taaaatatcc tcttaaaata tatgaagaag cttttgcagt taaaattaag atttttaagc
    11281 cgactaattt taaaaaaata tcgtccggaa atagtcgcca tttccgggtc tatcggcaag
    11341 actagcgcca aagaagctat ttttcaggtt ttgtcgggtc ggttagcggt gcgaacgagt
    11401 attaaaaatt ataataacga aattggcgtg cctttgacta taatcggagc ggagtcccct
    11461 ggttcatctt tcgttggctg gttacgggta tttgggcgcg cttggctttt gtatttccgg
    11521 cgcgatttgt cttatcctaa aattctcatt ttagaaatgg gtattgaccg gcctggcgat
    11581 atggattatc tgacgcgctt ggctccgccg acagtcggcg tcattactgc tgtcagttat
    11641 tctcatttag aatattttgg tagtttccaa aatatcaaga aggagaagca gattttaata
    11701 gaaagagtcg ctcgcaaggg tttaagtatt cttaattatg ataatgaagc tgcccgttca
    11761 atggccgagg tcagtccggc gcgggttctt tctttcggtc ttttacctgg cgccgatatc
    11821 gttgctcaag atttacacta tcgttaccaa ggagcgtctt atgatattgc tggtttaaat
    11881 tttaaactca attatgaagg caatattgtt cctgtggaaa tgcctaatat tataggggaa
    11941 gggggtgttt atgccgcttt ggctgccacc gcggtggcac tgcatttcgg tttaaatttg
    12001 atggaaattg cttcggcttt gcgcaattta cgcttaccgg ccgggaggat gcagcttcta
    12061 ccaggcatta aacatacttt tttgattgat gacagttata attcttcccc tgaagcgtgc
    12121 ctatccgcct taaaggtttt aggccagatc aagatagacg gcgctgccag taaatacgca
    12181 gttttaggag acatgttgga aatcggcgct tatagcgaag agggtcatgc tttggtcggt
    12241 cgtactttat ttgagcaggg gattaactat ctggtgagcg taggtgaacg ttctcgcgat
    12301 attgatcgcg gggccaaaga agcgggaatg agtagtgact ttatttttca ttttgatgat
    12361 tcggccacgg cggggcgatt tgttcaggaa cgcttgcatg aaggcgatgt cgtgttgatt
    12421 aaaggctctc aggggacaag aatggaaagg gtgactaaag aattaatggc cgaaccggat
    12481 aaagcggagg aattacttgt ccggcaggga ccaggctggc aggattagtt ttttcttaat
    12541 taagcgccat gacggcgctt tttgcttgat ttttcggact actaggctat actcaaatta
    12601 tgtctattct tattcttggc gccgccggca atctcggcgg tcagctaaaa aaggtttttt
    12661 ccaaccaaga ttgtcaagct tgggacaggc ttgattttga ttttttaaat tttgaagaat
    12721 tatccaagcg tttacatact ctatctccaa gcgttattat taatgccgcc gcctataatg
    12781 ccgttgataa atgcgaagag aacgaagctg aggcgatgct ggctaggcgt ttaaatactg
    12841 aacttccagg ggttttgtct gattattgct tgagtagtca aactctactc atccattatt
    12901 caaccgatta tgtttttggt ggagacggac gcttaagcgg tccttatcag gaaacagata
    12961 agccttcacc cttaaatgtt tatggacgta ccaaatttgc tggcgaacaa gaaatcgcca
    13021 gaagggcgct aaacggttta gattattatt taattcgcac ttctaaatta ttcggaccaa
    13081 aaggagaaag tccgcaagta aagcccagtt tttttgatat catgattact ttatccttgg
    13141 aaaaggaaac aatcaaagcg attgatgggg agaggagttg ttttacctat actcccgatt
    13201 tagcgattgc caccaagagt ttagtaacag atcgaccagc gcggggaata tatcacttgt
    13261 gtaattcagg cgaggcgact tggtatgatg gcgctaatta tctttttcag gctatcggcg
    13321 ctaccgttaa tttgcaggca ttggcgtcgg cagactggcc tcgtccggct gttcgccctc
    13381 aatattcagc tttagctaat catcgccgtc cgtctttgcg cccttggact gaagctttag
    13441 acgaatattt attttattta ggtttgaaga aataattaag tcaaatatat ttatgcgtgg
    13501 aattatttta tcgggaggca gtggcacccg cttacgccca ttaaccaaaa ttaccagtaa
    13561 gcagttgtta ccgatttata atcggccaat gatttattat cctttggaca ctttaattaa
    13621 agccggtatt aaagaaattc ttattattgt ttctccggaa agagcgggcg attatcttaa
    13681 tttactaggt agtggccggg aatttggtgt aaaatttacc tatgaaattc aagataagcc
    13741 cgaaggatta gctcaagctt ttattattgg tgaaaaattt attcaagggg agaacgtggt
    13801 gatgattttg ggcgataata tttttgaaga taattttgcc gatgatattt ctaattttaa
    13861 gagtggcgct aaagtatttg ctaaatatgt acctgacccg gagcgttttg gcgtcgttaa
    13921 attcgacgac aaaatgaagg cggaaaaaat tattgaaaaa ccgactaatt acttaagtaa
    13981 ttacgcggtt accggtcttt atgtttatga ccatcgtgtg gtcgaggtgg ctaaaaattt
    14041 aaaaccaagt gctcgcggcg aattagagat tacagattta aataattggt acctagccaa
    14101 aggcgagctg gaggtggcaa tggtggaggg agaatggttg gacgccggta cttttgacag
    14161 cttactcaag gcgcaattat ttgccaagga aaagttggac gctaagatgt ccatttaaat
    14221 tttccagtca ccttgtattt atatgaaatt agccgttgcc tttttatgtt acaatgacag
    14281 ttccgctcct tacttgccgg agtttttaaa gagtttagag gcggctttaa tacgtcttga
    14341 cgctgaaatt tttgtttttg ctggcgataa tagcgaccgc gcgccttttg tcaatcaaga
    14401 attattattt cgtcataatt taaaggcttc ttttcctatt aaactattgg cttttgataa
    14461 aaatttaggt tttgccgccg cttataatcg tttaattatc gctgctaaag gggtgggggc
    14521 agattatttt ttaatgatta atcccgacat gttgctgacc gagaatatga ttgcgcgctt
    14581 attatctgtt ttggcgaagc gaacagattt agccgcagtt tgtcctaaaa tatatcgttg
    14641 ggattttagg ggtaaacgtt taactaagat cattgattct tgcggtatag gcttggcgcc
    14701 gggtttgcgt ttttatgatc tcgggcaagg gaaagaagat aaggggcaat atgatagaac
    14761 tgaaattatt ggtccatcgg gagcagcggc tctttttcgc atcagttctt tggaaaaagt
    14821 gacagtggac ggtaaatatt ttgatgagcg tttttttatg tataaggagg attgcgacct
    14881 tgcttatcgc ttgtttcgag ctggtcagaa gacagtgttg gtgccagacg ccatcgctta
    14941 tcatgatcgg agtagtgctg gtcaggaaaa tttatggcag acttggcggc attggcaaaa
    15001 aaggagccgg ctgactcgtt cctggtcttt tgctaatcag aatctaattt ttgttaaata
    15061 ttttcatttt gaaagctatt tttcccgttt tttaattgtt gtaaaggtta tctttatggg
    15121 gttattttct ttaatattcg ccccatttct cttaaaaacc tattttaagt tttggggtgt
    15181 aaatcgcagt ataaatcgaa gcattgacta atataaaata ttatgataga ttaattttat
    15241 aaaattatta agattaaaat agtatagcga gtctatattt ttgtttatat ggataatttt
    15301 ttagaaagca ttgtgcgcga taagcgtagt gaggaattat ctaaattgaa tgcgattcaa
    15361 atagtcaata gtttgttctc cgacttaatg gacagagagc gtgatatttt gactaggcgt
    15421 tttggtttaa atggtgataa aaatgaaaca ttggagaaga ttggtcagct tcataaactg
    15481 actcgggaaa gggttcgcca gattgaaaac gcaagcatca aaaaaatcaa acgtttggat
    15541 aatttaagta attatctagt gactttaaag agtgtagttg aggagttatt aaaggagcat
    15601 ggtggtttat tgagacgcga ttttttactt gatattttaa ccgtgatgag tttagaaatg
    15661 aacaatgatg tcgacattaa tagcgctgaa tatgaaaaga ataggcaggt ttacaaaaat
    15721 cgttttaatt ttatcttatc caaacttctt gatgatgatt ttgatttaat gcccgactcc
    15781 agtaatttta atgcgtcggt taagcttaag gaagagtctt tagagcattt tgaaaaatta
    15841 tctaaggatt tattatcaaa acttgaatct ttgaataaga cgtttgatac tgaaggcgtt
    15901 ctgtcattgc ttaagagcct ggattcctat tcccagtatc aggataagct ggaggcaaat
    15961 aatagcgctg acatcacccc gatttttaaa agtcagattt ttcctgataa ggccgagatt
    16021 atcaacagta ataagacttt atactcctta atgcaggcgc tgaaagattt ggagcgcaat
    16081 aaatttggtt attggggtaa ggctgattgg caggaaatta aaccgaaaac cattaatgat
    16141 aagatttatt tagtcttaaa gaatgctggt aagccacttc attttacaga aattgcttct
    16201 aaaattaatg agataaaatt tgaccataag aatgccaatc cagcgactgt tcataatgaa
    16261 ttaattcttg atgaacgcta tgtcttaacc ggtcgaggaa tgtatggttt aagagaatgg
    16321 caaaatgctt aaagacttta ttattgcgaa ttaattgtag aatatttcat aaccgttgcg
    16381 ctcgctgcag cggtttttgt tttcttaaat ttatgctaaa atattgatat aattaccggc
    16441 gccttagatg acgtcgttgt ttagcctttt gttatggaaa ttacgattaa tacttccaga
    16501 ttggacgcct ttttcgcttt gccagctgat cagatgctgt gggtattttt tattaatatc
    16561 ggctggatgg ttattgccgc tgttttgtta tatggcatct ctttaattta tcttcaaaaa
    16621 atcagatctc agtgggcggc caagaatttg caatttatcc ttctggcaat cgatattccg
    16681 aagggtaatg aacaatcgcc taaagccgcc gagaacatgt ttagctattt agccggcgct
    16741 catggttcta ctaatttttt tgaaaaatgg tttgagggta aatttcaggt ctccttcagt
    16801 tacgaaattg ttagcattga gggttatact caatttttaa ttaggacgcc cgttgaattt
    16861 cgtgatttaa ttgaaacgtc tgtctattcc caatatcctg atgccgagat ttccgaggtt
    16921 gatgattatg ttgatactgt tcctcatcat tttccggatg aggattatga tgtttggggg
    16981 gcagaattca tacagtctaa caattgggcc tttcccatta agctttatca ggaatttgaa
    17041 cataattttg gcgaatcgga aacgcagttt aaagatccga tggcatcttt aatggatttg
    17101 tgcagttctt tgcgggaagg cgagcagtta tggatgcaac tggtgattat tcccataggt
    17161 tttgattggg tggcagaaac ggataaggtg gttgataaaa ttttaggcaa gaagaaaaaa
    17221 caatcaagtg ccgcttctaa atttgtggaa tggctgggca atatcagcga agctatttat
    17281 agtatctggg gcgatattga aagcaagtcc accgaagata agggcttatc aatgcaagaa
    17341 cttacaccta agcagaagag gcagatagag gccatccatt taaaagccgc aaaactatct
    17401 ttcgaggcaa aaattcgcgt agtttatgtg gctcgtaaag atttaatgaa caagaataag
    17461 gtggtaaacg gtgtaattgg ctatatgaaa cagtttgctt ctcttgattt aaacaatcta
    17521 aagccggatg ttaaattgac aatgactaaa actgcttatt ttaataaaga taagcgcctg
    17581 ctacgcaaaa agaatgcgat tatggctaat tatatcgcca gagaagatac ggcggggcgc
    17641 aatcctggtt tatataatat tgaggaattg gcaactctat ggcatttccc gatagaagct
    17701 aatgtcaagg cgccgttaat tcagaaagct cctggcagaa aagcggatgc cccttctact
    17761 ttgccgatag ttaatgatat ggccgctccc ttattggaac cattcagtgt ggaaattttg
    17821 catagcgcga ctagtcaatc acagagcgag agtaaaaatc atataaagga agaagaaaaa
    17881 cagaatgtgc ctgataattt accatttgtt taatgtttaa taagaaaaca aaaaaaagaa
    17941 tcggtattga tgctcgtttt tatggtcctc taggtaaggg cttggggcgt tatgttcaag
    18001 aagtggttga taatatcgta tcgatgaccg tcactagcag tgaatttgaa tatgttattt
    18061 ttttgagtcc ggataatttt aaagatttta agtctagcca tcccaatgtt cgcaaaatag
    18121 aagttccttg gcgttggtat tcatggcggg agcaattatt ttttcctttg attttaaaaa
    18181 aagaaaagtt agatttggtc cactttcccc atttcaatgt ccccatattg accccggtgc
    18241 cctttgtcgt cactattcat gatttaatac taacccgttt ctccagtcgc cgcgcctcaa
    18301 tgctcccagc agctttttat tggtttaagc aagccgccta tcgcttggtc attagaacgg
    18361 ctataaggcg agcccgagcc ataattaccg tttctgaatt tacccgtaga gatattatca
    18421 aaaagtttaa ggctaatcct aaaaaattat ttgttactta cgaaggggta gcggacttag
    18481 acaaaaaaga aattattggc gaaatagagg agaaacaagt tttacaacgt tatcggatta
    18541 gcgaaccctt tttgttatat gtgggcaacg cttatcctca taaaaattta gaagttttgc
    18601 tgactgtctt taataatttg ctttcccgcc atcatcatct caagttggta atggtgggta
    18661 aagacgatta tttttatcat cgtgttaagg aaaaggcgtc cgccctatcg ctttggcaaa
    18721 gcgaaggtag tgataataat aaagttattt ttccgggata tgttcctgat gccgatttag
    18781 ccgtgttgta tcgccgggct ttattttatg tttttccttc tttatatgaa ggttttggtt
    18841 tgccgccttt agaagcaatg agtcattctt gtccggttgt ttcttctaat caggctagtt
    18901 tgccggaagt tattggtgat gccgctcttt attttgatcc ctataaccaa gcggatatgt
    18961 tatctaaatt agaattttta tttgaaaatg aagattaccg ccaacaattg atagcgcgcg
    19021 gtttaaaaag agttaatgtt tttagctggc aaaaatgtgc gcgccaaact ttagaaatat
    19081 atcgtaagat attagtagaa taattttaaa attgatgaat cgggaaaata taaaaaaatc
    19141 taatataaag cgtaatttgt cggcagtcga ctcttttatc gtggatttgc gcccctctgg
    19201 tcagaaaaaa ctttcagaga gccaaaagcc ggttaatatt gcttttatta attggcaaaa
    19261 atttaaaaga atattttctt ttcgtcgacc gccgtatgcg gctactctat cttttttcaa
    19321 atctaattcg aacttagtct cgcccagtaa aaattcttct ttttctctac gtcatttttt
    19381 taattttcag gcaaagaagc gccgtcattt ccgtatttcg cgctcacatt atctccgcgg
    19441 ttctcgtttt aataagagag agcgtcgctt gtttgccgcc gccgctggtg tgcgttggcg
    19501 tcgcctcaaa aaagagaaat caaaaacaaa cgcccgctta tcccaagcgg agcatcttgt
    19561 ttcttggtac cggtctctct ttgtttttgt gtccgtttta ataattatca ttttgccttt
    19621 taaaattttg tcttatctaa aaattttgga catcaaaagt ttacaggctg gtgtgattga
    19681 ccgttcttta tccgctttaa ataatttagc ggccgcttcc gacggggcgg cgcaattaga
    19741 ttttagcgct gccgcctcta attttgctaa agcggagagt gattttatct cggctcaggc
    19801 ggaattaagt aggattgacg actggttgtt gtcgttcgct tctttttctg gtgatcctaa
    19861 aatcaaattg gcttcttctg gaaataaatt tttagcggcc ggtcaggcgg ccgctgcgat
    19921 gggtgagcat ttaagtgaag cgggaaatat cttattacaa acaagcgctg ataagaattg
    19981 gggaagcttg attgattctt ttattgaacc tggacagcag gctttaattg atgccgagga
    20041 gctacaacgt caacttgata atattgatgt cgatacttta ccagaggaat atcggagtca
    20101 atttatcgat tttcgcgatc aagccgaaat cgcggtttct tcactgcgct ctttactggg
    20161 ctcagctcaa gaaattaaag attttttagg agtgagtcga gataagcgct atttattggt
    20221 gtttcaaaat aatgcggaaa tgagggggtc tggtggattt ttgggtagtt atgcactggt
    20281 cgatatccgt gagggccgga ttcgtcgttt ggaagtgcca gctggcggat cctacgacac
    20341 tgaggcgggt atgaaaaatt ttatccaatc cccccaacct ttatggttag tgagtccgcg
    20401 ctggtatttt tgggatgcca attggtggcc ggattggccc accagcgctc gttccttgat
    20461 gtggttttat gaaaaaagcg gcggtccaac cgttgatggg gtgattagtt tcactcccga
    20521 tgttttatct gatttactaa aaattaccgg accgattgat atgcaagccg aatatggtct
    20581 aactattgat gctgataatt tttgggattt gattcaagcg acagtggaaa aagaaaattt
    20641 aataaaaacc catccagaag aagtggctaa tttacctgat agccccaaga atcaacccaa
    20701 gaaaattatt ggcgatttaa tggcggagat tatagaccgc ctacccagaa tattaacttt
    20761 tgaaaatctt cctttactta taaccgcctt ggaaaagaat ctcagttcta aaaatatcat
    20821 gctgtatttt tccgatcctt tgttacaggc aaaagccgcg cgttatggtt ttgacggagc
    20881 gatgttgccg agtcagcatg attatcttct tattgcccac accaatattg ccggccagaa
    20941 aagtgatcgc aagatgcttg atgaggttaa acattccgtt cagattctta gtgaccgcag
    21001 cttaatcgac acccttacca ttactcgtac tcatactggc ttgaaaaatg aaattttgag
    21061 cggtgtccgg aatgttgatt ggttaagggt atatgtaccc gctggtagcc aattattaga
    21121 agcccgcggt tttcgcgctc ccgacgcttc ttattttgaa aagccggagg agggttggga
    21181 aaaatttcct ttaatcgctg aaaccgaaga actcgctttg accgatttag taagcggcac
    21241 taagatttat aaagaaaatg gaaaaaccgt ttttgctaat tgggtaatgg ttgatccggg
    21301 ggaaacagtg gagataaaat tacgctatcg tttgcctttt cgcttggagg cggaggccga
    21361 cgtcagtggc ggcgattggc tcacccgact tggctcttta ttggataaag ctcccggcga
    21421 acgtttcccc ttctctttat tgttgcaaaa acagccaggc gctaaggcta ctagtgttga
    21481 cttggaaatt acggctccat ccggctggcg gcagatctgg aactatccta atcaatcttc
    21541 ttggaatggc aacctatccc tagatagaga cgtaatgcgg gccgttttat tggagaaata
    21601 atatcaagtt tatttttagc gatttttaag aattataatt ttttaacggg cttcggtagt
    21661 tgcgactatc gtcgcatcgt tcttaattac atgacagtct tgaaaaaaaa gaaaaaaaca
    21721 tcttccgcag ataaggattc tggtttaaag acttttttag aaagaccttt accggccgtt
    21781 gaagaagttt cccgctttga agaggcggta aaaaaagaag cagccgctga agaggtggaa
    21841 aataatcttt ctgctattta tcacgacaag cagggacgtc tagttgatgt tagccgagtc
    21901 cagaaaagac accgtttgcg tttgttagtt ttatttaaac aagtttttat tctcactctt
    21961 ttagcttgcg gactctatgc gggatattat tactattttc agcgtcccgc cggtactagt
    22021 gccattattt taacagtaca agccccagag aaagctttgg ccggagaacc aatatcttat
    22081 aatattacct acagtaatga tagcggttta gccctaagta acgctaagtt agaggtgctt
    22141 ttgccgtcaa attttataat tactgaaagc gtgcccgctc cttccggcat taatacctgg
    22201 agttttggcg atatcgctat tggcggtagc ggactagttg tcattagcgg ttatttaaac
    22261 tcgccagttg attccgctaa tgtggttagc gcccgcttgt cttatgtgcc ggccaatttt
    22321 tccagtgagt ttaagaagga ggccagcgcg aatacggtag tctccggttt gggctttatc
    22381 gtcagcgccg attatcttaa tacagctatt attggtcaaa acaatgaaat gaagttgttt
    22441 ttgaacgcct ttaaaaataa taagctatcc gatctttatc taaaaatttc cggctcagat
    22501 aattttatcg tcaatcaagt ctcttcagag aagggcgaac tcgtgtccga aaataatcag
    22561 tcagagggcg cggctgctga tgtttttaaa attcagaata ccggtgagaa ctcttggttt
    22621 ttgtccggtt tgccggcaga ttctgaggag aagataacga tagctatcga ttttacatta
    22681 aagaccaaga atcaggataa agaagattta agtttgcgct tgataaaaag agaggccgac
    22741 ggccgggaga tgtctttatg ggaaaagact atatcttttg aggctattaa gagcgattta
    22801 aatttaagcc ttactttaaa cggttctaag acagatcaga ctttaaattt tggtgacacc
    22861 ttatcttatc aattagagta tgctaataat ggtgaggcta ctcttgatga cgtcgcttta
    22921 atggctgtta ttaaaggtga ttttgttgat tggtcttctt tgcgcgaccc tctgggcggc
    22981 agtgttgccg gcgatgcgat tgtctggact aaagagcagt tgccatcatt gtctgaattg
    23041 actcctggcg ccagcggtaa aatagatttt tctttaaaat tacgtaattt ttccgtttcc
    23101 gatttaggcg ttgacactag tatcacgagt tatgctcaat atggtttaaa taataaccaa
    23161 gagggcgctt ctgaagataa tcggagtaat accatcaagg ggcaaataaa tagtgatttg
    23221 tcattgagtg aaaagatttt gtattttaac gaagataata tcccggtggg ttctggtcca
    23281 ttgccgcctc gagtcggcga aaagactagt gtgagagttt tttggactct aaaaaataat
    23341 ttacatgatt tgagcgatgt ccaagtagtt ttagatttac ctcaagggct ggaatggtca
    23401 ggcggggaaa atactaatgt cggtagtatc acctatgatg ccgagagtaa tcaagttact
    23461 tggcgtttgg gttttttgcc tatttctgtg tatcgcgctg atgccgagtt taatatcagt
    23521 tttatcccta aagatagtga tcgcgataaa attttagtaa tttctcccgg tagcacggtc
    23581 acagcgcttg attctttgac tggcggcagg ataagttata agaccaaagc taagaccagt
    23641 aagcttgaag acgatgaaat tgccggtttt tctaataatg gacgagtaga ataattatga
    23701 gcggatttaa attaaagcta tttaaagacg gtcttcgtcg cgggcgtgta actaatattc
    23761 atggttcttt atcaacgcct ttttttatgc cggacgctac tagagccttt gtgaaagcta
    23821 ataccagcgc ggacgctaag agggcgggta ccgaggtgat ggtggtcaac acttttcacc
    23881 tctatcttca gcccggacaa aagataatta agcgcgccgg cggcgtccat aattttatgg
    23941 attggcaagg gccgctttta tctgattccg gcggttttca ggttttttct ttgattcatc
    24001 aaggaaaaaa tctgggaaaa attaatgacg ataaagttat tttcaaatcg cctatcgacg
    24061 gttctcggca cgaatttagt ccggaaaagt ctatccaaat tcaatttgat ttaggtgttg
    24121 atatgatggt ttgtctggac gactgtccgc ccaatgattg gccgagggcc gatttagaaa
    24181 aagctgttaa gcgtagtttg gcttgggcgg cgcgctgtaa gcaggaatac cttaaacaga
    24241 taaaaaggcg gaaaataact gaagataata gacctttgct gtttgccgtg gttcagggcg
    24301 gagattatga agatttacgc cgagaaagcg cacgtcagtt gcttgctatt ggttttgacg
    24361 gctatggttt tggcgccaga ccggttgata aagatggtaa ttttttagcc gaaactattt
    24421 ttaaaactgc ttcattttta cctaaagatt ctttgcgctt cgctctcggc gttggcatgc
    24481 ccgaggatat ttatcgttgc tatctgatgg gctgggatat gtttgattgt gttattccta
    24541 ctcgtgaagg taggcacggc aaattatttt ttttcaagcc tggattccgg gggtttacaa
    24601 aaagaaagac gaagccggga ccagtcgtgt cttatcctga tttctatcgg acaaagaata
    24661 tcaacaatgc cgcctttgct caagattttt ccccgattaa tgctgacagc gatctgccgg
    24721 aactgcgccg ctacagtaag gcttttttgc atcatctttt ccgtttggat gaacctttag
    24781 gacaaaaatt agctagtctt aataatttgg aattttataa tcgcctgtta agagaactgc
    24841 gtcgcttgta ataatcgtca tcttttattt tttctttttt tgttttataa tgctgttgct
    24901 aaacaaatta tatttatata a
//
LOCUS       DAOQSA010000016        20298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_1819_length_20298_cov_8.325940, whole genome shotgun sequence.
ACCESSION   DAOQSA010000016 DAOQSA010000000
VERSION     DAOQSA010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 20298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20298
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1819_length_20298_cov_8.325940"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="PKK65_02510"
     rRNA            <1..980
                     /locus_tag="PKK65_02510"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1034..1148
                     /gene="rrf"
                     /locus_tag="PKK65_02515"
     rRNA            1034..1148
                     /gene="rrf"
                     /locus_tag="PKK65_02515"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            1278..2672
                     /gene="glmM"
                     /locus_tag="PKK65_02520"
     CDS             1278..2672
                     /gene="glmM"
                     /locus_tag="PKK65_02520"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006987766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNU81588.1"
                     /translation="MTLIKSISGIRGTIGGRPEENLTPLDLVAFLSAYAQQLKQKFPK
                     FGDLSVVLGRDGRISGPLLHQLASSVLRFNGISVLDVGLATTPTVEMAVIREKAQGGI
                     ILSASHNLRQWNALKLLNEKGEFLNAEDGEAILTLAKNSAFIYADIDSLGREIKVNDA
                     LESHIAAITALKLVRTQDIAKRNFKIVVDGINSVGALAIPTLLESLGVKDIEIINGDI
                     NGEFAHNPEPLDQFLEEIKQRVVNVGADLGIVVDPDVDRLAFIDEKGKMFGEEYTLVA
                     VADYVMHDYCPCFYQKISVSNLSSSRALKDITEKRGGAYYAAAVGEVNVVNKMKETGA
                     VIGGEGNGGVIYPELHYGRDALVGTALFLSFLAQEGVSVSELKRRYPQYQMIKDRIDL
                     NQETDVKALLIKIKEHYQGEQINDMDGLKIDWPDSWVHLRASNTEPIIRIYAEANTYE
                     EASRRVQKIKEIIA"
     gene            3040..3780
                     /locus_tag="PKK65_02525"
     CDS             3040..3780
                     /locus_tag="PKK65_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNU81589.1"
                     /translation="MKTKIIILAAGKGTRMGSDLPKALVQLDGRPMIIHLIESVVASN
                     IDDLPIVVVSPDNREIISKALAEYNCDYVIQEQPLGSGHAVACARQKIPANTDNVIVL
                     YCDHPFITAKSIKNFGEFITDAVTIMPTELGDYDDWHQIFFHWGRIVRDNKNEIEKII
                     EFKDASPEEIKITEVNPGFMCFNNKWLWDNIDFLDNNNGSKEYYLTSLVAMAFKQGRK
                     IGTINIEPREAMGINSQEELAIAESLVS"
     gene            3783..5042
                     /locus_tag="PKK65_02530"
     CDS             3783..5042
                     /locus_tag="PKK65_02530"
                     /inference="COORDINATES: protein motif:HMM:NF024660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HNU81590.1"
                     /translation="MRRILDLHLHSRYSRACSPRLTLVNIAQTAQTKGVDIIATGDFT
                     HPAWFEAISKELEEIGNTGLYHLKDGSSKTLFLLSTEVSLVYKDGGKVRRLHLVVHAP
                     NLEAVKELKEKLGASYNIRSDGRPILGISAPDFVKLCLHIHPQFLVYPAHIWTPWYAV
                     FGSKSGFDSLEECFHEQTEFIYAYETGLSSDPEMNWRLSALDKLTCLSNSDAHSLENI
                     GREANVMELTSASYENIYQIIKGNKRLVAGDVEGMIETIEFYPEEGMYHLDGHRDCHF
                     SCNPEESRRLHNLCPHCGRPLIIGVSSRVAELADRPLGFKPDKAVPFRRLVELDKIIA
                     ESLQVKNRKSIKVNIVYQELIKKFYSELSILMDLDLTILKSDYPVVAAGIERVRQGQL
                     TIIPGFDGQYGLIQVFPDNFFKLKTLF"
     gene            5049..5522
                     /locus_tag="PKK65_02535"
     CDS             5049..5522
                     /locus_tag="PKK65_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81591.1"
                     /translation="MRRIISQIIILVLFLFLTVFASVWFEDTGGYENTETTRSQSILW
                     FSKAAQKTRQMINGLAGIAVPSASETVKENKRDSVVAAEAVSNLEEFTQDLGSMASAS
                     TLDSFNSDSNFRQFLANFFSKAPSLYFDTKVYSNGWRNFWSLYWLEYNNLRPEQP"
     gene            5740..6762
                     /gene="recA"
                     /locus_tag="PKK65_02540"
     CDS             5740..6762
                     /gene="recA"
                     /locus_tag="PKK65_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNU81592.1"
                     /translation="MVNDKKAEAAANAMEQIKTKFGEGAIMKFGEVRKTNVDAISTGC
                     LSLDIAFGIGGVPRGRVIEIFGPESSGKTTLAQHIVAEVQKLGGIAAFVDAEHALDPD
                     YAARIGVNINELLISQPDSGEQALDIVETLVRSNAVDIIVVDSVAALVPQKEIEGEMG
                     DQHMGLQARLMSQALRKLTGIIAKTKTSVIFINQIRNKIGVFFGNPETTTGGNALKFY
                     SSVRVEVRRAAQIKQGENIIGNRVKVKIVKNKVAAPFKTCEFDIMYNEGISMAGDLVD
                     AGVEYGVIKKTGNSYTFGDIKLGVGRENAKKTIKEDDKLQASLRKAILAEVKSKEIES
                     TSTTKK"
     gene            6812..7489
                     /gene="pyrH"
                     /locus_tag="PKK65_02545"
     CDS             6812..7489
                     /gene="pyrH"
                     /locus_tag="PKK65_02545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNU81593.1"
                     /translation="MTYILSVGGSLIVPATGIDINFLKSFRRFVLKRYDASDRLIIVA
                     GGGVTARQYVQAAAAVETVPEDDQDWLGIHATRLNAHLLRILLKDVAYPEIITNPDKA
                     LHTRSRVIVAGGYRPGWSTDYVAVLLANKYKAKTVINLSNIDYVYNQDPRRYPDAKII
                     KKINWDDFRQIVGNEWHPGLNAPFDPIAARLAQSSRLRVVILNGRNFVNLNKFFDNKV
                     FKGTIIQ"
     gene            complement(7555..8112)
                     /locus_tag="PKK65_02550"
     CDS             complement(7555..8112)
                     /locus_tag="PKK65_02550"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNU81594.1"
                     /translation="MRNFIIFGPPGAGKGTQAQKIANEFNLHHLSTGALLREEISRQT
                     EIGQKVQKIMDQGGLVDDSIVNAIVKDKITQNSQGDGFIFDGYPRTMAQAKNLDALLT
                     AKALPLVLNLEVEEKELMERLKLRGQESGRSDDNEKTVKNRLRLYSEQTRPLLDFYAR
                     QKRLISVNGQGTIEEVFQLLRNQIK"
     gene            complement(8144..8216)
                     /locus_tag="PKK65_02555"
     tRNA            complement(8144..8216)
                     /locus_tag="PKK65_02555"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8181..8183),aa:Lys,seq:ctt)
     gene            complement(8288..8361)
                     /locus_tag="PKK65_02560"
     tRNA            complement(8288..8361)
                     /locus_tag="PKK65_02560"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8325..8327),aa:Met,seq:cat)
     gene            complement(8500..8573)
                     /locus_tag="PKK65_02565"
     tRNA            complement(8500..8573)
                     /locus_tag="PKK65_02565"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8537..8539),aa:Arg,seq:tcg)
     gene            complement(8642..8929)
                     /locus_tag="PKK65_02570"
     CDS             complement(8642..8929)
                     /locus_tag="PKK65_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004350606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNU81595.1"
                     /translation="MNKAQLIDKLYHEVEGLNKKQTEKMLDVLTDTIIEELKNDREVT
                     ITGFGTFLSRVRYSRGGVNPQNPKERITIPAVKVAKFKTGYNLKQALKGKR"
     gene            complement(8976..10490)
                     /gene="rny"
                     /locus_tag="PKK65_02575"
     CDS             complement(8976..10490)
                     /gene="rny"
                     /locus_tag="PKK65_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNU81596.1"
                     /translation="MEYVIAAIAVVGGFFIGTWFQQKKTSRRIKGAEKKADNIISEAK
                     VKEKELLLKAQDKALKIIEEAKREEAQRRKEINGLQNRLEQRESAFSQKLLDLQEKQQ
                     KLYDKVNEVQEIKERIKTIKEEQAKKLEEMANLTQEEAKDLLLKNIEEKAEEDLMIRV
                     RKLEEDNNDKLQEKAKDIMALAMQRMVSSYTVELTTTTIDLPNDEMKGRIIGREGRNI
                     KAIEQMTGVEIIVDDTPNAITVSGFSLIRRHIAKKTLDYLIKDGRIHPTKIEEAVEMA
                     KKELAVDIKKAGEEAMYEVGITGFDPKLVQILGRLKYRTSYGQNTLSHSIEVAHLAAM
                     LAEELGSDVTLAKKGGLLHDIGKAVDHEVQGNHTEIGRDIAKKFNLPEEIIAPIATHH
                     DDQPATLTSVIVKVADAISSARPGARSDSHENYIQRLEELEKIATSFEGIEKAYAIQA
                     GREIRVFVKPEAIDDFKAHNLARDIAKKIETELKYPGEIKINVIRETRVIEYAR"
     gene            10873..12858
                     /gene="infB"
                     /locus_tag="PKK65_02580"
     CDS             10873..12858
                     /gene="infB"
                     /locus_tag="PKK65_02580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNU81597.1"
                     /translation="MNITELARILRISPQELRDLLPELGFAIGQKAIKVDNNTAKKII
                     KDWPIFRRQWEQRKAVERAKKQDEAILPKEKKTILIPPVITVRALAEISQIPVNRLLQ
                     ELIKNGVFTSINEKIDFDTAVIIGADLNLDIQLLAETDADEKTKEEKKLKDILDSEKP
                     EDLQSRPPVIVVMGHVDHGKTKLLDSIRLTNVIAGETGGITQHIGAYQVARKDRKLTF
                     IDTPGHEAFTAMRSRGAKVADIAILVVAADDGVKPQTVEAFRIIEAAKLPFLVAINKI
                     DKPEADVMKTKQDLANQLNITPEDWGGKTICVPISAKEGTGVEELLDMVILTADAELG
                     DSLRANPEAEAAGTVVESNVDKAAGPVATLLVQNGTLRVGDQLCFEDVVYGRVKSLKN
                     YKGEEIKEAGPSVPVKILGLKISPSVGDILYVGAGKKLKMRKIKSTDVKKEKIMSIAQ
                     PENADDATAKVNLVIKSDFLGSAEAIEESLMKINTEKVKVKIVAKGLGNVTEGDVKRA
                     EDSNSQIICFNTKMAPNVENLARSKNIDVHFFSIIYDLIKYVKEEMQKLVPEEIIRIE
                     LGKLKVLAIFRTEKQQQIIGGKVLSGRPENEAIVNVWRGEDFITTGKITGLQSAKQEV
                     KFVEEGEECGLKFEGQPVILEGDILEFYREDHNIEKI"
     gene            12860..13216
                     /locus_tag="PKK65_02585"
     CDS             12860..13216
                     /locus_tag="PKK65_02585"
                     /inference="COORDINATES: protein motif:HMM:NF014130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0030490 - maturation of SSU-rRNA [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HNU81598.1"
                     /translation="MSKVEQFNELLHRELAVAINRELELPEVFLTISYVSCTPDLQQA
                     KVAVSVWPDKMAGTALKKLKASSGRIVSGIIKKTRLRKVPRLLWEFDSTEKKAADLEE
                     VFLKIDSEGDNNGHVF"
     gene            13310..14344
                     /locus_tag="PKK65_02590"
     CDS             13310..14344
                     /locus_tag="PKK65_02590"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HNU81599.1"
                     /translation="MEMQAKLIKALEMMRSSERILIIAHLRPDGDALSSVAALRLLGL
                     NLGKEVSSFCQDKNQELFDYLPGVNTIYSDLGSLKKGLGINDSLRDYFDLIIVADCGS
                     LARTAIGDDILEFKHKGGQVIEFDHHPRIDDYADLEIRYPELSSTAELVYNFIVANKL
                     SFNRDLADCILTGILTDTGNFLYPSASDATMKAAAAALAAGARYNKIANYVWRNKDMA
                     MIKMWGLALERLKLNPRYQLAFAVITRDDIINIFGQDNLDSAAESDFFSGLAGFLSNL
                     SGAKAVMLLYEDRRGLVKGSLRSTPNGMLVGKLARALGGGGHERAAGFTLPGTLEQID
                     NQWVLVDNKI"
     gene            complement(14546..15217)
                     /gene="trmD"
                     /locus_tag="PKK65_02595"
     CDS             complement(14546..15217)
                     /gene="trmD"
                     /locus_tag="PKK65_02595"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNU81600.1"
                     /translation="MKFKILSIFPDILNSYLEDSIIKRAVAKKIIKIEKYDLRNWTND
                     RHRTVDDAPYGGGAGMLMKIEPLYQALKEIKKKSRVDVKKRKIILLSASGKTWNQKLA
                     KQYSCLEEIIFICGRYEGVDARIKKFIDEEISIGDYVLTGGELPALVIIDSITRLLPG
                     VLGNSASIEEESHNKKNVLEYPQYTRPAIFVAEGKKYAVPKVLLKGNHAQIKDWRHKK
                     QKTKK"
     gene            complement(15418..15540)
                     /locus_tag="PKK65_02600"
     CDS             complement(15418..15540)
                     /locus_tag="PKK65_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81601.1"
                     /translation="MDTKAILGILAAIIFIINGIPYLSDIHNKKISLHISSWFG"
     gene            complement(15604..15939)
                     /locus_tag="PKK65_02605"
     CDS             complement(15604..15939)
                     /locus_tag="PKK65_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNU81602.1"
                     /translation="MEADKQFLEMIVKSIVSNPNDVAVDRTVDEMGVLLTLKINPADM
                     GYVIGRKGQTAQAIRTLLKIVGAKNNSRVNLKIYDPEGGARRSNRPTNDSRSDLGDVD
                     TSALDDLKI"
     gene            complement(16077..16505)
                     /gene="rpsP"
                     /locus_tag="PKK65_02610"
     CDS             complement(16077..16505)
                     /gene="rpsP"
                     /locus_tag="PKK65_02610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNU81603.1"
                     /translation="MLMIKLSKIGKTNKKIFRLIISEKGRDPYGRSLEVLGSYNPYSK
                     NLEAKTDRIKYWLDKGAGMTASVNNLLVGKGVIEGNKVTASKPGKANEKKVAQAKAKN
                     DKKSVSEPVNAPATSAEKATTSTDPEVTVENQEEKAEAKA"
     gene            complement(16609..18873)
                     /locus_tag="PKK65_02615"
     CDS             complement(16609..18873)
                     /locus_tag="PKK65_02615"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNU81604.1"
                     /translation="MEKIVSCDWLGRQLTIKTGKIALQADAAVLVQYGETVVMATVVQ
                     NKSAREGTDFFPLMVEFQEKLYAAGIIKGSRWIKREGRPTDDSVLTGRMIDRSIRPLF
                     NQDDRRDVQVIITVLAVDQENDYDVVSLVASSAALAISGLEWRGPIGGIRVGRIEGNF
                     VFNPTYAEQAASDLDLIVAGTKNKTIMIEAGANEVPETVMLEAILAGQKNMKPAITLI
                     EELQKQIEPKNNKVKQVLKDEEELKKEADKQKNLNLGSTWLKENIPNILFDKTYYTKG
                     ERKAAVNAIKEQLNSYLFEKGVSADQRDDVVKSLVEKMIDEAITASILENGKRVDGRA
                     LNEIRPLSAEVALLPRDHGTSLFSRGETQVMSIVTLGAPSMEQSLEGMEGQSKKRYMH
                     HYNFPPYSVGEAAPLRGTGRREIGHGALAEKALLPMIPSREDFPYIIRVVSETLGSNG
                     SSSMASTCASTLALMDAGVPIKKPVVGIAMGLASYHDLSKWQVLTDIQDLEDGDGGMD
                     FKITGTDAGLTAIQLDTKTEGLTPEIIAQTFKQGREALNQILEVIKNTIPAPRQELSP
                     YAPRITSFHVDPEKIGTIIGPGGKVINKIIEETGVSIDIDDDGLVMVCGTDAAMVTKA
                     VNQIQDIVHVFAVGEVVTGKVVRMMDFGAFVQLNNSQDGLVRASEITPYYLEKPGDIL
                     NIGDTVTVKITEIDDQGRINLTMKGLIENADLWKDEKGKGDPANRMNNFRRPGGDNRD
                     RFASRNRAPRRPRF"
     gene            complement(18987..19496)
                     /locus_tag="PKK65_02620"
     CDS             complement(18987..19496)
                     /locus_tag="PKK65_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNU81605.1"
                     /translation="MIKHKEQRVGVLIDVANMYHSAKNLYGRRVNFKEILTKAVADRK
                     LIRATAYVIRAENDEETTFFEALSQQGFEVKMKDLQIFAGGAKKADWDVGIAIDAIKL
                     ADKLDVIVLVSGDGDYLPLVSYLQNNKGCLVEVMAFRKTCSARLIEEVDDFIDLGSDN
                     KFLIGNKYK"
     gene            complement(19628..19891)
                     /gene="rpsO"
                     /locus_tag="PKK65_02625"
                     /pseudo
     CDS             complement(19628..19891)
                     /gene="rpsO"
                     /locus_tag="PKK65_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548131.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
     gene            complement(19964..20224)
                     /gene="rpsT"
                     /locus_tag="PKK65_02630"
     CDS             complement(19964..20224)
                     /gene="rpsT"
                     /locus_tag="PKK65_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNU81606.1"
                     /translation="MPNKKSAKKELRKSVKREAANLKVKNVMKTAIKKSLKKVQATDK
                     SVKEDLAVVMKNIDKAAKKGVIKKRTAARKKSRLAKKINKLK"
BASE COUNT         5632 a         3775 c         4397 g         6494 t
ORIGIN      
        1 caagggttgg gctgttcgcc cattaaagcg gcacgcgagc tgggttcaga ccgtttagca
       61 gctaataata ttggctatta aacgctctga atccagaggt aagacatcat atacggcggc
      121 aatatcgagt aattgatatt ggcataaaaa gctgactcat atcgatggaa tcctaacagg
      181 caatagaaaa ttaacctgtc atatgttagg ataatatcga gggaagtcgc ttatgttaaa
      241 attaacgcaa actcaattag cttacttagc aggatttatc gatggtgata gtagtattta
      301 tgtaagattg aaaccgaatg atacttatcg tttatcatta tggctttcag attgcttctt
      361 acatagctct ctttcaatca gccaaagatg aaaaaaattt ccaaaaattt gtaacttgtt
      421 aagttgtggc tatttgcgta agagaaaaga cggaatatta gaatatatta tcaatcgcaa
      481 attagaagaa atttttagaa aaaaagattt agtaaaaaat atcaaagatt tttctaaatt
      541 aatggattta atcaataagt ttgtaaagat aaattattct aaaaaacgca gcaaacataa
      601 gttgaccccg tagagactaa acgtcggcct cctgaaagcg tttcaggatg aaagcatagt
      661 ccatgcactt agtaataagt gaaaacatgc gtaagacagg tcggtctcct atccactgtg
      721 gtcgtggaaa cttgaagagg cccttcctta gtacgagagg accaggaagg acgaagctct
      781 agtgtacctg ttgttctacc aagggcattg cagggtggct accttcggtt aagataagcg
      841 ctgaaagcat ctaagcgcga agctgtctct aagattaggt ttcacagact ggttaaagaa
      901 tatgaccttg ataggctgta tgtgtaagtc cagtaatgga ttgagcaaag cagtactaat
      961 aagtcgtcag attttcccac acttttgttt ttcaaataaa actcttcgaa aacccataaa
     1021 aagacgccat tgttctggtg gttaaagcgg aggggtcaca cctgatccct tcccgaactc
     1081 agaagttaag ccctccagcg ccgatgatac ttgggcctga gccctgggaa agtaggtcgc
     1141 cgccagaaca atggtgtttt ttatgggaaa cttagaagct atttaaaaaa cgccggtaaa
     1201 tccggtgttt ttttcttggc tttattttca tttattcgta taatgttaat aatatcaatt
     1261 tttaaaagta aattaatatg accttaataa aatcaatctc cggaattcgc ggaactatcg
     1321 gcggtcgacc tgaagaaaat ttaacccctt tggatttagt agcttttctt tccgcttatg
     1381 ctcagcaatt aaagcaaaaa ttccctaaat ttggagattt atctgtggtt ttgggtcgtg
     1441 atggccgtat cagcggcccc ttattacatc aattagccag ctcggtttta cgttttaacg
     1501 gcatatccgt actagatgtt ggtttagcga caacacctac tgtagagatg gctgttatca
     1561 gggaaaaagc tcaaggcggt attattctta gcgcctccca taatcttcgc cagtggaacg
     1621 cgctcaaatt attaaatgaa aaaggtgagt ttctaaacgc tgaagacgga gaagctattt
     1681 tgaccttagc taagaattcc gcttttattt atgccgatat tgattctctg ggtagagaga
     1741 taaaagttaa tgatgccctg gaaagccaca tagcggcgat tacggcgtta aagttggtga
     1801 ggactcagga tattgccaaa cgtaatttta aaattgtcgt cgacggcatt aattcggttg
     1861 gtgctttagc gattcccaca ctgttagaaa gtttaggcgt gaaagatatc gaaataatta
     1921 atggtgatat taatggtgag tttgctcata atcctgagcc attagatcaa tttctggaag
     1981 aaattaagca aagagtcgta aatgtcggcg ctgatttagg tatcgtggtc gatcccgacg
     2041 tcgatcgcct agcttttatt gatgaaaaag ggaagatgtt tggtgaagaa tatactctag
     2101 tggcggtggc agattatgtt atgcatgatt attgcccgtg tttttaccaa aaaatttctg
     2161 tctctaattt atcttcttcg cgagctctta aagatatcac tgaaaagagg ggtggcgcct
     2221 attatgccgc cgctgtcggc gaggtgaatg tggtgaacaa gatgaaggaa acgggagcgg
     2281 ttatcggcgg tgagggtaat ggcggcgtga tttatccgga attacattac ggtcgagatg
     2341 ccttggttgg caccgcttta tttttaagtt tcttagccca agaaggagtt agtgtttcag
     2401 agttgaaaag gcggtatcca caatatcaga tgattaaaga cagaattgat cttaaccagg
     2461 aaacagatgt taaggcttta ttgataaaaa ttaaagagca ttatcaggga gaacaaatca
     2521 atgatatgga tgggctgaag attgattggc ccgattcctg ggtgcattta cgcgcttcta
     2581 acacggaacc aattatcaga atttatgcgg aggcgaatac ttatgaagaa gcttctcggc
     2641 gagttcaaaa gattaaagaa attattgctt aaaataaaaa gccggattcc caaaggagaa
     2701 aagccaagaa atgtttgtgc ccatattttt acgctcaatt ctattgatgt gagcagaaaa
     2761 aagaagttga agccttaaag atgaaagtaa ttaaaaatta aggtaataac catttttatg
     2821 gtgtttcagc tgcataatag attcaggccg gaaaaatggt tggtagaatt agcggttttt
     2881 gccaaattat tctttgtttt catggtacta tggtttttag atttcatatt tttgtataat
     2941 attaaaataa atttgttaat attctctaat ttttcagggt agtaattttg ggtcggtaat
     3001 gctaaaatat taatatataa agtaagaaaa attgttgtta tgaagacaaa aatcattatt
     3061 ttagcggccg gtaagggcac gcgtatgggc tcagatttgc caaaagcatt ggttcagctt
     3121 gatggtcggc cgatgattat tcatttaatt gaatcggttg ttgcttctaa tattgatgat
     3181 ttacccattg tcgtggtttc tccggataat agagaaataa ttagtaaggc gctagcggag
     3241 tataactgtg attatgttat ccaagaacaa ccgttaggga gcggccatgc ggtcgcttgt
     3301 gcccgtcaaa aaattccagc aaatacagat aatgtaatcg tcttgtattg cgatcatcct
     3361 tttataacgg ccaagtcaat taaaaatttt ggagaattta ttactgacgc cgtgacgata
     3421 atgccgaccg aattaggcga ttatgatgat tggcatcaga ttttttttca ctggggaaga
     3481 atcgttaggg acaataaaaa tgagattgaa aaaattatcg aatttaaaga cgcttcgcct
     3541 gaagagatta aaattacgga agttaacccc ggctttatgt gttttaataa taaatggctg
     3601 tgggataata ttgattttct tgataataac aacggatcaa aagaatatta tttaacctca
     3661 ctagtagcaa tggcttttaa gcagggacgc aaaattggaa caataaatat tgagcctcgt
     3721 gaggcgatgg gtattaacag ccaggaagag ctggcaattg ccgagagttt agtctcttaa
     3781 atatgcgccg gattttagat ttacatcttc attctcgtta ttctcgcgcc tgttcgccgc
     3841 gtttgacgtt ggttaatatt gcccaaacag cgcaaacaaa aggagttgat attattgcca
     3901 ccggagattt tacccatccc gcttggtttg aggcaatttc caaagaacta gaagaaattg
     3961 gtaacaccgg tctttatcat ttgaaagatg gttctagcaa aactcttttt ttattaagca
     4021 cagaagtgtc cttggtgtat aaagacggcg gcaaggtcag gaggctgcac ctggtcgtcc
     4081 acgctccaaa tttagaggcg gttaaagaat taaaggaaaa acttggcgct tcctataata
     4141 tccgttcaga cggtcggcct attttaggca taagcgctcc agattttgtt aagctctgtt
     4201 tacatatcca tccacaattt ttagtttacc ctgctcatat ttggacgcct tggtatgccg
     4261 tgttcggttc caagtccggt tttgattctt tagaagagtg ttttcatgaa cagacggagt
     4321 ttatctatgc ttatgagacg ggtctgtcta gtgacccgga aatgaattgg cgcctaagcg
     4381 ctcttgataa attaacatgt ttgtccaact ccgatgccca cagtctggaa aatattggtc
     4441 gcgaagccaa tgtcatggaa ctgacaagcg ctagttatga gaatatttat caaataatta
     4501 aagggaacaa gcgcctagtg gctggcgatg ttgaaggcat gattgagact attgaatttt
     4561 atccggagga gggtatgtat catcttgatg gccatcgtga ttgtcatttt agctgcaatc
     4621 cagaagaaag ccggcgccta cataatcttt gcccacactg tggccgacca ttgattatcg
     4681 gcgtttctag ccgtgtggcg gaattagctg atcgcccttt aggatttaag cctgataagg
     4741 cagtaccttt tcgacgtctg gttgaattag ataaaatcat cgctgaatct ttacaagtca
     4801 agaatcgtaa atcaataaaa gttaacatcg tctatcaaga gttgattaaa aaattctatt
     4861 cagagctttc gattttaatg gatttagact taacaatttt aaaaagcgat taccctgtag
     4921 tggctgctgg tattgagcgc gtccgccaag ggcagcttac tattatcccc ggatttgatg
     4981 ggcagtatgg attaatacaa gtttttcctg ataatttttt caagttgaaa actttatttt
     5041 aaaaatatat gagaagaatt atttcgcaaa ttatcattct cgtattattc ctttttctca
     5101 cggtttttgc ttctgtctgg tttgaagata ccggtggtta tgaaaacacc gagacaacca
     5161 gatcgcaatc aattttatgg tttagtaagg cggcgcaaaa gactcggcag atgattaacg
     5221 gtttagccgg tatcgccgtt ccttcggctt cggaaaccgt aaaagaaaat aagagagata
     5281 gcgtcgtcgc tgctgaagcc gtttcaaact tagaagagtt tactcaagat ttaggttcaa
     5341 tggcaagcgc ttcgactctt gatagcttta attctgatag taattttagg caatttttag
     5401 ccaatttttt tagcaaagcc ccttctttat attttgatac aaaggtctat agcaatggtt
     5461 ggcgtaattt ctggtcttta tattggctag aatacaataa tttgaggccg gaacaacctt
     5521 aagtagctat taaaaaagta ttttttatag tataataatt gagccacact gagtgtttat
     5581 cgatatttta ggaagggttt agcggcggtg tgttatcatt catttattaa taaagtaatt
     5641 tgcgtctacg ggcggatagc cggcggctgc cgttatgacg ctatatttat gcctaaagaa
     5701 aaaaaggcgg ttattgtcaa agaagaaacc gctaactata tggttaatga taagaaagcg
     5761 gaggcggcgg ccaatgcgat ggaacagatt aagacaaaat ttggcgaagg ggcgattatg
     5821 aaatttggcg aggttagaaa aactaatgta gacgccatct ctaccggttg tttgtcttta
     5881 gatattgctt tcggtatcgg tggtgtcccc cgtggtcgtg ttattgaaat ttttggtcca
     5941 gaatcatctg ggaagaccac attggctcag catattgtgg cagaggtgca gaaattaggg
     6001 ggaattgccg cttttgtgga cgctgaacat gctttggatc cagattacgc ggctagaatc
     6061 ggagtcaata ttaatgagct gcttatttct cagccagatt ccggcgagca ggctttggat
     6121 attgtcgaaa cactagttcg ctctaatgct gttgacatta ttgttgtaga tagtgtcgcc
     6181 gccttagtgc cgcaaaagga aattgaaggc gagatgggcg atcagcacat gggcctgcaa
     6241 gcgcgtttga tgagccaagc tctgcgcaaa ctaaccggga taattgccaa gactaaaact
     6301 agtgttattt ttattaatca aatacgtaat aaaattggtg tcttttttgg taatcccgag
     6361 actacaactg gcggtaatgc tttgaaattc tactcttccg tcagggtgga agtccgtcgc
     6421 gctgctcaaa ttaagcaggg agagaatatt attggtaatc gggttaaagt gaagattgta
     6481 aaaaataaag tggccgctcc ttttaaaacc tgtgaatttg acatcatgta taatgagggc
     6541 atttccatgg ccggtgattt ggttgacgct ggtgttgagt atggagtcat taaaaagacc
     6601 ggaaattcct atacttttgg ggatataaaa ttaggagtcg gacgcgaaaa tgctaaaaaa
     6661 acaattaagg aagacgataa gctacaggct tctttgcgca aggccatatt agctgaggtt
     6721 aaaagcaaag aaatagaatc aaccagcact actaagaaat aatttttatc attacggatg
     6781 tcttggcagc gggcgtccat ttttgtacgc tatgacttat attttatctg tcggtggttc
     6841 cctgattgtt cccgccaccg ggatcgatat taatttttta aaatctttcc gtcgttttgt
     6901 gcttaaacgt tatgacgctt ccgatcgttt gattattgtc gctggtggcg gggtcaccgc
     6961 cagacaatat gttcaagcgg ctgccgccgt agagacggtt cctgaagatg accaggattg
     7021 gttgggaata catgctaccc gccttaatgc tcatttactg agaattttat tgaaagatgt
     7081 cgcctatcca gaaataatca ctaatcctga taaagcttta cacacccgtt cccgggttat
     7141 tgtggccggt ggttatcgtc ccggctggtc gaccgattac gttgcggttt tattagctaa
     7201 taaatataaa gctaaaacgg taattaatct ttccaatatt gattatgttt ataatcagga
     7261 tcctcgccgt tatccagatg ccaaaattat aaaaaagata aattgggatg attttcgtca
     7321 gattgttggc aatgagtggc acccgggctt aaatgccccc tttgacccga tagcggcgcg
     7381 tttggcgcaa tcttctcgat tgcgggtagt gattctcaat ggtcgtaatt ttgttaattt
     7441 aaataaattt tttgataata aagtttttaa aggcacaatt atccagtgag ctgattaggg
     7501 cttaaacaaa aaataaaaaa gaaccaacaa atggttcttt ttttatcgct ttttttattt
     7561 tatttggttt cgtagtaatt ggaagacttc ttcaattgtg ccctggccat taacactaat
     7621 taggcgtttt tggcgagcat agaaatcaag taaagggcga gtctgttcgc tatagaggcg
     7681 gagacgattt ttaacggtct tttcattatc atcgctgcgg ccagattctt gtccgcgcaa
     7741 ttttagtctt tccattaatt ctttttcttc tacctccaag tttaaaacta acgggagagc
     7801 ctttgcggta aggagtgcgt ctagattttt tgcctgagcc attgttctgg gatatccgtc
     7861 aaaaataaaa ccgtctcctt ggctgttttg tgtgatttta tctttaacaa tagcattgac
     7921 gatgctgtcg tctactaatc ccccttggtc cattattttt tgcacttttt gtccgatttc
     7981 agtttgacgg gaaatttctt cccgcaatag cgctcctgta gagaggtggt gtaaattgaa
     8041 ttcattggct atcttttgtg cctgtgtccc cttgccagct cctggcggtc caaatataat
     8101 aaaatttcgc ataaatatta gctaaatgaa ctatatttat cggtgcgccc agagggattc
     8161 gaacccacga ccatctcctt aagagggagt tgctctacca gctgagctat aggcgcatta
     8221 tcattattat gttcaatcta attgttatgg ttaataattt ttgaactttg tttgtaatta
     8281 atttctgtgg ctggggaggg gctcgaaccc tcgaccttag cgttatgagt gctacgctct
     8341 aaccagctga gctacccagc ctaaaattct atattattaa gtgctgctac gcatctgatg
     8401 acttaatggg tagtgatcaa gtcaatttaa ccgtgcatgt atgggactat tatatttatc
     8461 atcagcctaa gagtcaatac ctgaaaagga caggtttagt gctccggaga ggactcgaac
     8521 ctctaacctt gtggtccgaa gccacacgct ctatccaatt gagctaccgg agctttatta
     8581 tcggcataaa gcttgcccgg agagagcccc gggcaagctt ttaaagcaaa taatatttat
     8641 tttagcgctt acctttcagc gcttgtttga ggttgtagcc agttttgaat ttggccactt
     8701 taaccgccgg aatggtaatt ctttctttgg ggttttgagg attgacacct ccacgagagt
     8761 agcgaactcg ggaaagaaag gtgccaaaac cggtgatggt aacctcgcgg tcatttttta
     8821 gctcctcaat gatggtgtcg gttaagacat caagcatttt ttccgtttgt ttcttgttca
     8881 ggccctctac ttcgtggtag agtttgtcga ttaattgcgc tttattcata tatttggcgc
     8941 ctaaccccag ttggggaggt ggcgttaatt ataatttatc tggcgtattc tattacccta
     9001 gtttctctga tgacgttgat tttgatttct ccagggtatt ttaattcagt ttctattttt
     9061 ttagcaatat ctctagctag attgtgagcc ttgaagtcat caatagcttc cggcttaaca
     9121 aagactctaa tttcgcgtcc ggcttggatg gcgtaggctt tttcaatgcc ctcaaagctg
     9181 gtggcaattt tttctaattc ttctaggcgt tgaatgtagt tttcgtggct atcgcttctg
     9241 gcccctggtc tggctgagga aatagcatcg gcgaccttga ctattactga ggtaagagtg
     9301 gcgggctggt catcatgatg agtggcgata ggggcaataa tttcttcggg taaattgaat
     9361 tttttagcga tgtcgcgtcc gatttcggta tgattacctt gaacttcatg atcaaccgcc
     9421 ttgccaatat catggagcag accacccttt ttagctaaag tgacatctga acccaattct
     9481 tcagctagca tcgccgccaa atgggcaact tcaattgaat gacttaaagt gttctggcca
     9541 taactcgtgc ggtattttaa acgtccaaga atctgtacta atttagggtc aaagccggta
     9601 atgccaactt cgtacatcgc ttcttcgccg gccttcttaa tgtcaaccgc cagctctttt
     9661 ttagccatct ctaccgcctc ttcaatttta gtaggatgaa tgcgcccgtc ttttatcaaa
     9721 taatctaggg tttttttggc gatatggcgt ctaattaagg aaaagccaga aacagtaata
     9781 gcattgggag tgtcgtcaac aattatttcg acgccggtca tttgttcaat ggctttaatg
     9841 ttccttcctt cgcgaccaat gatgcgccct ttcatttcgt cattaggtaa gtcaatcgtg
     9901 gtggtagtta attcaaccgt gtatgatgac accattctct gcatcgctag agccatgata
     9961 tccttggctt tttcttgtag cttgtcgtta ttatcttctt ctaacttgcg gacgcgaatc
    10021 attaagtcct cttccgcttt ttcttcaata tttttcaaca gtaaatcttt ggcttcttcc
    10081 tgagtgagat ttgccatttc ttccagtttc tttgcctgtt cttctttaat agttttaatg
    10141 cgctctttaa tttcttgaac ctcattaacc ttgtcgtata atttttgttg tttttcctgg
    10201 agatctaaca gtttttggga gaaggcgctt tctctttgtt caaggcgatt ctggagtccg
    10261 ttaatttctt tgcgtcgctg agcttcttct cgtttggctt cttcaatgat tttaagggct
    10321 ttgtcttggg ctttaagtaa tagctctttt tctttaacct tggcttcact aatgatgtta
    10381 tctgcttttt tttcagcgcc cttgatacgg cggctcgttt ttttctgttg aaaccaagta
    10441 ccaataaaaa aaccacccac aacagcgata gcagcgatta catattccat aaaagtaaag
    10501 accgaaaaca agaaagcatc tgaatctgta aaaaataaaa tttcctctta tattaaggtt
    10561 tttgtgtaat atgggtaaaa attgataaca acatgagtaa tcatttcagt gtttaggtaa
    10621 atataagata ataatgggaa taaaaacgtc cggttttagt gaggatttct tattatcagc
    10681 taaaatcggg cttatcttga atttcggcta attattaatt gctaagtaat attagtaatt
    10741 atcttagcaa ttatttttgt ctttgtaaag gcattttttg ctagtttttc attgactaaa
    10801 tttaaaaaat aaagtaatat ttaaaaataa ttaataaaat cagtctctaa ataagtcatt
    10861 tgttaaattt ttatgaatat tacagaatta gctagaattt tgcgcatttc tcctcaagaa
    10921 ttgcgcgatt tattgcctga acttggtttt gccatcggtc aaaaggcgat taaagtagat
    10981 aataataccg ccaaaaaaat tattaaagat tggccaattt ttagacgtca gtgggagcaa
    11041 agaaaggcgg ttgaacgtgc caagaagcaa gatgaggcta ttttgccaaa agaaaaaaaa
    11101 acaattttga ttccgcccgt cattactgtc cgcgctttag ctgaaatatc acaaattcca
    11161 gttaatcgct tattacaaga gcttattaag aatggcgttt ttacttctat taacgaaaag
    11221 attgattttg atacggccgt tattatcggt gccgatctta atttagatat ccagctttta
    11281 gcagaaaccg acgcggatga gaaaactaaa gaagaaaaaa aattaaaaga cattttagat
    11341 tcagagaagc cagaagattt acaatcccgc ccgcccgtca ttgttgttat ggggcacgtt
    11401 gaccatggga aaaccaaatt gcttgactcc attcgtttaa ctaatgttat cgcaggagag
    11461 acggggggta taactcagca cattggtgcc tatcaagtag cgcgcaaaga ccggaaactt
    11521 acttttatag acactcctgg tcatgaagct tttacggcga tgcgtagtcg tggcgccaag
    11581 gtggcggata tcgctatttt ggtagtggct gctgatgacg gcgttaaacc acaaacagtt
    11641 gaagctttcc gtattattga agcggcaaaa ttgccatttt tagtggctat taataaaatc
    11701 gataaaccag aagctgatgt gatgaaaacc aaacaagatt tagctaatca gttaaatatt
    11761 actccggaag attggggcgg caagactatc tgcgtgccca tttcagccaa ggagggcaca
    11821 ggagtagaag aattgcttga tatggttatt ttaacggcag atgcggaatt aggtgattct
    11881 ctaagggcta accccgaagc ggaagctgct ggtacagtgg ttgaatctaa tgttgataaa
    11941 gcggccggtc cggtagctac tttgttggtg cagaatggaa ctttgcgtgt aggcgaccag
    12001 ctttgttttg aggatgtagt ttatggtaga gttaaatctt taaagaatta taaaggcgaa
    12061 gaaattaaag aggctggacc gtcggtgccg gtaaaaattt taggattaaa gatttctcct
    12121 tcggtgggtg atattcttta tgtcggcgct ggcaaaaaat taaagatgcg taaaataaag
    12181 agcaccgatg ttaaaaagga aaaaataatg tctattgctc aaccagaaaa cgctgacgat
    12241 gctacggcta aagttaatct ggtaattaaa agtgattttt tgggttcggc agaggcgatt
    12301 gaagagtctc taatgaagat taatacggaa aaagtaaagg taaaaatagt cgctaagggt
    12361 ttaggtaatg ttaccgaggg cgacgttaag cgggccgagg attcaaattc tcagattatc
    12421 tgctttaata cgaaaatggc gcctaatgtg gaaaatttag ccaggagtaa aaatatcgat
    12481 gtccactttt tttccattat ttatgattta attaagtatg ttaaagagga aatgcagaag
    12541 ttggtaccgg aagaaataat taggattgaa ttaggtaaac ttaaagtttt agccattttc
    12601 cgtaccgaaa aacaacagca aattattggc ggcaaagttt tatctggccg cccggaaaat
    12661 gaggctatcg ttaatgtttg gcggggagag gattttatta ccaccggtaa aattactggc
    12721 ttgcagtcgg ctaagcaaga agttaaattt gtggaagagg gtgaggagtg cggattgaaa
    12781 tttgaaggtc agcctgtaat tttagagggt gacattttgg aattttatcg tgaagaccat
    12841 aacattgaga agatataata tgtctaaagt tgaacagttt aatgagcttt tacatcgcga
    12901 attagcagtc gccattaata gagagctgga actaccggag gtatttctga ctatttctta
    12961 tgtttcctgt actcctgatt tacagcaggc gaaggtggct gtgtctgtgt ggccggataa
    13021 gatggcggga actgctttga aaaaactaaa agcttcttca ggaagaatcg tgtcaggtat
    13081 tatcaaaaag acgcgtttgc gcaaggtgcc gcgtttatta tgggagtttg attccaccga
    13141 aaagaaagcg gctgatttgg aggaggtttt tttaaagatt gacagcgagg gagataataa
    13201 tggccatgtt ttctaattta ttgttattag ttatataaaa aggccgcctt tcgttagggc
    13261 ggttttttag ttttgacatt taggcaattt atcgataaaa tagccagtta tggaaatgca
    13321 ggcaaagtta atcaaggctt tagagatgat gcgttcgtca gagcgtattt taattattgc
    13381 tcatctccgt cccgacggcg acgccctgtc ttctgtcgcc gccctgcgtt tactcggtct
    13441 taatttaggc aaggaagtga gtagtttctg tcaggacaaa aatcaagagt tatttgatta
    13501 tctgcccgga gttaatacta tctatagtga tttaggctct ttaaagaagg ggttaggcat
    13561 taatgattct ttgcgtgatt attttgattt aattattgtt gccgactgcg gctctttggc
    13621 cagaacggcc ataggtgatg atattcttga atttaagcat aaaggcggcc aggttattga
    13681 gtttgatcat catccgcgta ttgatgatta cgctgactta gaaatacgtt atccagaatt
    13741 atcatctacc gctgaattag tctataactt tattgtcgcc aacaagctat cttttaatag
    13801 agatttggcc gattgtattt taaccggcat tttaaccgat accggtaatt ttctttatcc
    13861 ttccgcctct gatgctacca tgaaagcggc tgctgccgcc ttggcggccg gtgctcgtta
    13921 taataaaatt gccaactacg tctggcgtaa taaagatatg gctatgatta agatgtgggg
    13981 tctggcttta gagagactga aattgaatcc ccgctaccaa ctcgcttttg ctgttattac
    14041 gcgtgacgat ataataaata tttttggtca agataatctc gacagcgctg ctgaaagtga
    14101 tttttttagc ggcttagccg gttttttaag caatctctcc ggggccaagg cggtgatgtt
    14161 gctctatgaa gatcgtcgcg gattggtaaa aggcagcctg agaagtactc ccaacggtat
    14221 gctggtgggt aaattggcgc gcgctctagg tggtggcgga catgaaagag cggctggttt
    14281 tactttgcct ggcaccttag agcagataga taatcagtgg gtactggttg ataataagat
    14341 ataatgagca gttaaatata aggttcactt taatttgtgg agccgttaaa cggcgctacc
    14401 aatgaagtca ccgctaagga attaacgaaa atatccgatg ttttaaaatt atcggtctgg
    14461 atgcgggcaa cgtttccaga cctgactttt ttcagctgtc aagttaccat ttaattaccc
    14521 gactttatat cagcaaaatg gttatttatt tttttgtttt ttgttttttg tggcgccaat
    14581 cttttatttg ggcatgattt cctttaagca gaactttagg gacagcatat ttttttcctt
    14641 cggctacaaa gatagccggc cgggtatatt gcgggtactc taatacattt tttttgttgt
    14701 gtgattcttc ttcaatgctg gcgctgttac ccaaaacgcc tggtagcagt ctcgtaatag
    14761 aatcaattat tactagggcg gggagctcgc ccccggttaa gacatagtcg ccgatggaaa
    14821 tttcttcatc aataaatttt tttattctcg catcaacgcc ttcatagcgc ccgcagataa
    14881 aaattatttc ttcaagacaa ctatattgct tagccagttt ttgattccaa gtttttccgc
    14941 tggctgaaag tagtataatt tttctttttt taacatcaac cctactcttc ttctttatct
    15001 ctttaagcgc ttgatagagg ggttctattt tcattaacat ccccgctcct cctccgtaag
    15061 gagcgtcatc aacagtgcgg tgtctatcat ttgtccaatt tctaaggtcg tatttttcaa
    15121 ttttaataat ttttttagca acagcccttt taataatgct atcttcgaga taggagttaa
    15181 ggatgtctgg gaagatgctc aatattttaa atttcataat attaatccta aaatacttaa
    15241 gtcaaaaaaa taaaaaatta tatattgagg ttgaccatac ccaattttct taattattga
    15301 ataaattgaa taaaatgtaa tagtaaaaca tcggctggta ttagtggaaa gaagcgttag
    15361 cacccagcgg ttttcccctg ctagtctagc tgtgtcgcct aaagtggtaa taaaagatta
    15421 tccaaaccaa gatgaaatat gcagactgat ttttttatta tgaatatctg acagataggg
    15481 aatgccattg attataaaaa taatagccgc taaaattcct aaaatagctt tagtatccat
    15541 tcaattattt tataacaaaa aagccggatt gggaaatccg gctttttcta tctcgtttta
    15601 tgattaaatt ttgagatcat ccagagcgga agtatcgaca tcacccaagt ctgaacggct
    15661 atcgttagtc ggacgattgg aacgacgagc gccgccttcc gggtcataaa ttttcaggtt
    15721 gacgcgggag ttatttttag cgccgacaat ctttaacaag gtgcgaatcg cttgcgcggt
    15781 ctggcccttg cggccgataa cataacccat gtcagccgga ttgattttga gagttaaaag
    15841 tactcccatt tcatcaacgg tccgatcgac agcgacgtca ttgggattgc tgacgatgct
    15901 ttttacgatc atttcgagaa actgtttgtc tgcttccata tttgttattg ctgggctttc
    15961 aaatcaatcg cactagtgtc gctggacttg aaagatccta gacttaatta tattacctca
    16021 tttgcacgag atttatatca atattatctt atcaattata gcttatagat gtcaatttaa
    16081 gctttagcct cggctttttc ttcttgattt tcaacggtga cttcagggtc agtagaagtt
    16141 gtggctttct cggcagaagt tgccggggca ttcactggtt ctgacactga ttttttatca
    16201 ttcttagctt tagcctgagc aactttcttt tcgttagctt tgcccggttt agaagctgtt
    16261 accttgttgc cttctatcac tcctttaccg actaatagat tgttgacgct ggcggtcatg
    16321 ccggcgcctt tatccagcca gtatttaatc ctgtctgttt tagcttctaa atttttagaa
    16381 taggggttat atgaacctaa aacttccaaa gagcgtccat aagggtcgcg tcctttttca
    16441 gagataatta gacggaaaat ttttttgtta gttttgccga ttttggataa ttttatcatt
    16501 aacatataat attttagagg cgcctccgag cggtggaaaa tccagacgaa taacgcgatt
    16561 ctttttcttt attgacaaat attagcttaa ggcttgaaaa aagtcaagtt aaaagcgggg
    16621 tcggcgggga gcgcgattgc gactggcaaa gcgatcacga ttatcaccgc cgggacgacg
    16681 gaaattattc atacgattag ccgggtcgcc cttgcctttt tcatctttcc ataaatcagc
    16741 attttctatc aaacctttca tggtcaggtt tattctgcct tggtcatcaa tttcagttat
    16801 tttaacagtg acagtatcgc cgatatttaa gatatcgcca ggtttttcga ggtaatatgg
    16861 ggtgatttca ctggctctaa ccaagccgtc ctggctgttg tttaattgga caaaggcgcc
    16921 gaagtccatc atgcgcacta ctttaccggt gacaacttct ccgaccgcaa aaacgtggac
    16981 gatgtcctga atttgattga ccgctttagt caccatggcc gcatcagtgc cgcagaccat
    17041 tactaaaccg tcatcatcga tatcaatact aactccggtt tcctcgataa tcttattgat
    17101 aaccttgccg cccggtccga tgatagtgcc aattttttcc ggatcaacat ggaaactggt
    17161 aatgcgagga gcatagggag atagttcttg acgaggtgcg gggatggtat tttttatcac
    17221 ttccagaatt tgattcaaag cttctctgcc ttgtttgaag gtttgtgcaa taatttccgg
    17281 agttaaacct tcggtcttag tatctagctg aatggcggtt aagccggcgt cggtgccggt
    17341 aattttaaaa tccataccgc catcgccgtc ttccaaatct tgaatatcag tcaatacctg
    17401 ccatttactt aagtcgtgat aagaggcgag acccatggca atgcctacta ctggtttttt
    17461 aattggtacg ccagcatcca ttaaggctaa agtcgaagcg caggttgaag ccatcgagga
    17521 ggagccgtta gagcccaaag tctcactgac cacgcggatg atataaggaa aatcttcgcg
    17581 gctaggtatc atcggtaata aggctttttc tgctaaagcg ccatggccga tttcgcgccg
    17641 gccggtgccg cgtaaaggag cggcttcacc aacagaatag ggcggaaaat tataatgatg
    17701 catgtagcgt tttttgctct ggccttccat accttcaaga gattgttcca tgctgggagc
    17761 gcctaaagta acaatagaca ttacctgagt ttcgccgcga gaaaataggg aagtgccgtg
    17821 atcgcgaggg agtaacgcta cttccgcact aagcggacgg atttcgttaa gagcgcgacc
    17881 gtcaacccgt ttgccgtttt ccaaaataga agcggtgata gcttcatcaa tcattttctc
    17941 taccaatgat ttaacaacat cgtcgcgttg gtcggcactg actccttttt caaaaagata
    18001 agagttaagt tgttctttga tggcgttcac tgccgccttg cgctctcctt tggtgtaata
    18061 ggttttatcg aaaagaatat tggggatgtt ttctttaagc catgtcgagc ctaaatttag
    18121 atttttctgt ttatctgctt cttttttcaa ctcttcttcg tcttttagaa cttgtttaac
    18181 tttattattc ttgggctcta tttgtttttg taattcttca attaaagtaa tcgccggttt
    18241 catatttttt tgtccagcca ggattgcttc tagcataact gtctcgggga cctcattggc
    18301 gcccgcttca atcatgattg ttttattctt ggtaccggca acaattaaat ccaagtcgct
    18361 ggcggcttgt tcggcataag tggggttaaa aacaaaatta ccttctatgc ggccgaccct
    18421 aattccgcca atcggtccgc gccattccag tccagagata gctagggccg cagaagaagc
    18481 gactaaggaa acgacatcat agtcattttc ttggtcaacc gctaagacgg tgatgattac
    18541 ctgaacgtcg cgacggtcgt cttgattaaa aagagggcgg atagagcgat caatcatgcg
    18601 cccggtcagc accgaatcat cagtcggcct accttcgcgc ttaatccagc gagaaccctt
    18661 aataatacca gcggcataga gtttttcttg aaattcgacc attaatggga aaaaatccgt
    18721 tccttcgcga gcggatttat tttggacaac tgtggccata actacagttt ctccatattg
    18781 tactaaaacg gcggcatccg cctggagggc gattttgcct gttttaatgg tgagctggcg
    18841 gccaagccag tcgcaactta ctattttttc cataattttg tgagcttggc ctacaaatta
    18901 cagaactgta tatccttccg cgctcaaatt taaagcgggc agcctacagt actatctatt
    18961 tgcagaccaa aactttagtt tattttttat ttatatttat taccaattag aaatttatta
    19021 tcagaaccca aatcaataaa gtcatccact tcttcaatta agcgagcaga acaggttttt
    19081 ctaaaagcca taacctctac cagacagcct ttattgttct gtaagtatga gaccaaaggt
    19141 agataatctc catcgccgga gaccagcacg atgacgtcta atttatcggc gagtttaatc
    19201 gcgtcaatag cgatgccgac atcccaatca gcttttttag cgccaccggc aaaaatctgt
    19261 aaatctttca ttttcacttc aaacccttgt tggctaagag cttcaaagaa agtcgtttct
    19321 tcatcgtttt ctgcccggat aacataagca gtggcccgga ttaatttccg gtcggccact
    19381 gctttagtca agatttcctt aaagttaacg cgtctgccat aaaggttttt ggcggagtga
    19441 tacatattgg cgacatctat caagacgccg acgcgttgtt ctttatgttt tatcatattt
    19501 attcgccgtc ttcctcggta tcggggttga ttttcatatt ttcttcttcg tcttcttcgg
    19561 aagcgatatt gtatttcttt tccttcaatt tttcttcttc ttcttcagct tgtagtttct
    19621 gggcgatttt tagtttaagt ttgtcggcca attccttaaa ttgttcggca ttttccttct
    19681 gtaaatattt aaggagtttg cggcgttcgc cgactttttt caataaaccg cggcgggaag
    19741 aataatcgtg tttatgctgt tttaagtgtt cggttaattg cttgatttct tcggttaaaa
    19801 tagcaatttg gacttgcggg gagccggtat cggtattatg gacacgaaat tttttaatga
    19861 ttttttcttt gtttttttta tctaacataa tttcttcgct tattcccaga aaaggggttt
    19921 gcacccgttt ccgagaaaaa gtgcattaat atttaaggct caattatttg agcttattga
    19981 tttttttagc taagcgtgat tttttgcggg cggccgtcct tttcttaatt actccttttt
    20041 tggccgcttt atcgatattt ttcataacga cagccaagtc ttccttaacg cttttatcag
    20101 tcgcctggac tttttttaaa ctttttttaa tagcggtttt catgacattt ttgactttga
    20161 gattggcggc ttctctcttt acgctcttgc gcagttcttt tttcgccgat ttcttattag
    20221 gcatacgaaa ttttgataag ttaggtaaat tattaaaagt attcttaggt tagcataaaa
    20281 ctaaaaaaaa atcaagac
//
LOCUS       DAOQSA010000017        16773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_2526_length_16773_cov_8.922240, whole genome shotgun sequence.
ACCESSION   DAOQSA010000017 DAOQSA010000000
VERSION     DAOQSA010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16773
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2526_length_16773_cov_8.922240"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PKK65_02635"
     CDS             <1..212
                     /locus_tag="PKK65_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81607.1"
                     /translation="VEEIDNLISNIKGLLKEGGNCRITPISTQNEKAKQAMLYNLKQI
                     VDSKEYNIHANKDTLIIHKLFINRN"
     gene            248..1513
                     /locus_tag="PKK65_02640"
     CDS             248..1513
                     /locus_tag="PKK65_02640"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNU81608.1"
                     /translation="MTIKNKPELLAPAGNLEKLKTALAHGADAVYCGLPDFSLRTRVN
                     DFSLEDIKEGIVYAHALGKKVYITLNIIARNNHLAKLKSHVRKLKTLKPDAIFIADPG
                     VLEIVKAIWPGARLSLSTQANCTNIASARFWAKQGFKRLILSRELGIKEIAAIKAGLK
                     NTEIEVFVHGALCSSYSGRCFLSDYFSGRSANLGDCAQPCRWEYEIKPYGHEKSLIVG
                     EDTHGTYFLNSKDLCLLERLPEIVHAGVNALKIEGRAKSVYYLANVVGAYRRALDLIA
                     AGKAKAVLRKELSFLKSELTEKLNQRGYTEGFMFTREDNLQNYEGNFPDSPWEFCGQV
                     ISSLKQGKHFILLVKVHNTLLADDELEIIAPAYRVNQIKAAAMRDAETGDEIYEAHGG
                     GGNSHILITSAKNWPAYSVLRRRLRKTSS"
     gene            complement(1549..2025)
                     /locus_tag="PKK65_02645"
     CDS             complement(1549..2025)
                     /locus_tag="PKK65_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81609.1"
                     /translation="MNKSMLTGLGFGLTSAVITTLGLMIGLNASTGSKLAVIGGILTI
                     ALADAFSDALGVHVSKETEKGAIAKEVLAATGFTFLFKFIFALTFVVPVIFFSLSTAI
                     IISIVWGLSILSLLSYFLAKSQGQSRLESLGEHLVIAIAVLLLSNGIGSVIAKYFN"
     gene            2069..3205
                     /locus_tag="PKK65_02650"
     CDS             2069..3205
                     /locus_tag="PKK65_02650"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNU81610.1"
                     /translation="MSLWREKNKKFLFLLLGSLVFLCSLFFYQISGNGLEVRPDINSE
                     RNEKDKNEKDKEEKIWPELPMRPPLVLLFGGDVMLSRTVNAKMAAYDDYEWPFAQISQ
                     LTSSADITIFNLESPFLKDADYNVPSGSFMFKADPKAIIGLQAAGVDVLSLANNHIMN
                     MGDRGLDDTLSILQMAGIGAVGAGQNEKAARQGYLWTGKGWKVAFLSYAYPDDNSVAG
                     EKKPGISTMDVDNLREDIARWRHQADLVIVLMHAGTEYVSSPNKEQVTFARAAIDAGA
                     DAVIGHHPHWPQSWEIYQNKPIFYSLGNLVFDQMWSRETSQGLLARLTFNDNFSGQAE
                     LWPIIISDYGQAQLWPEAVPESIFWDAYDLPIPADLSWPALGDN"
     gene            complement(3135..3998)
                     /locus_tag="PKK65_02655"
     CDS             complement(3135..3998)
                     /locus_tag="PKK65_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81611.1"
                     /translation="MKNKRKEIYQLAENIKKDWPKPLVGALEKFWLETLRDDHKGLIS
                     TLVLSILPPHPSTKQKDAAIYLGAASLYRWTAANLQDDLLDDRTTKTINLPLVKACWE
                     SAVALSLAVPLEPTGKNFLLKLSLRENNALFSEVKQPKTIPRGILAPSGKSLFLLIAP
                     LHLSLFLGWKKTETQGLFRAGRYLLAAKQLADDLYDYRDDWQNGRRNFAHRGLKRLPE
                     KKELPSYYRRQAEAILQLCGQCRKITKTISPLSKKNCFEPLLKPLEINCRRGLAKINQ
                     LESVNHRHPKK"
     gene            complement(3995..5368)
                     /locus_tag="PKK65_02660"
     CDS             complement(3995..5368)
                     /locus_tag="PKK65_02660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNU81612.1"
                     /translation="MKFKKIWAAWLKSIKSTSPAEKKLEKSLAISIVIMFLLNLIRLH
                     DFIINPDNQGLPLSATLGLLAFFIILQILFKKGYRNLSAWLLITAYTLPTIFCLSYWG
                     ADLPAALLMTVLIIMLAGIFLNTKAALTIASIFAGAIIGLNWLQTNQIIKVNNDWRQQ
                     PMQLADALSYVIVLGLIFGLAWMIVKENKQTFLSLKHKQEELNKINEELEIKVAERTK
                     AVKIMQREKLEQLQALASIGQLTSSIFHDIINPLTIVSLNLEQIETKGDCEIEKSNVY
                     VQQALSATARINELINSANRCLRRQNQEKYFSVRQEIEKIQKIMEGKARASQTIIQLE
                     AEGDYYIKGGPIRFGQIFMNLLSNAIDACAQNKPTAGIIKITIKKQTDSNKLLISVKD
                     NGIGIPPDHLHKIFNIFFTTKPEDSKNVGLGLSIVKEIIENDFQGAIAVDSQLGKGSA
                     FTVSLPI"
     gene            complement(5643..6314)
                     /locus_tag="PKK65_02665"
     CDS             complement(5643..6314)
                     /locus_tag="PKK65_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNU81613.1"
                     /translation="MRLLIIEDEKNLAELLGSLLKKAGFAVDIANDGEKGSFMARTQA
                     YDLIITDYVLPKLDGGSIIKEIRDDGRNVPIIMLSVRQSIIDKINILDIGADDYLSKP
                     FSGEELLARIRALLRRPQTIKEEKLIFADLELEPKSFKVRRANKDIRLTNKEFSLLQH
                     LLLNAGKIVSRESLLEHVWSGDVDPFSNTVETHILRLRRKIDNHQPKLIHSIIGRGYK
                     LDTSI"
     gene            6666..8750
                     /gene="mrdA"
                     /locus_tag="PKK65_02670"
     CDS             6666..8750
                     /gene="mrdA"
                     /locus_tag="PKK65_02670"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNU81614.1"
                     /translation="MAVLKKKRFFLGDFRSSRGKGNDPFVIKEGNFKFGQLKDSAYYT
                     DWTEHSFLSDSRDKEVVGRTFDVKKILPIKIIILLFILILAGRTAWLQLIRGSHYFGL
                     AEGNRLRLENIEPKRGIIYDRKRRALVRNTANFVLSLRPIDLPRDELARDELLRRISF
                     IIDDMPAEPTLNANFDLVSDGPSFQLMKNAIAGISPFSLEAYQPVFIADNIGYEQALH
                     LLASIDSLPGVIIDTKIRREYPFTADQEDGTPSDLSSLSHILGYTGKITLEALNRLGS
                     QYSLVDYVGKTGLEYSWEKELKGINGHKNIEVDAFGRRKKVVSELEPVAGYNLKLSLD
                     LDLQRQAEIVTKSWLLKTKTSRAAVIAMDPRNGQILALVSLPAYNNNLFARGLSQEEY
                     DVFLNDENHPLFNRAISGELPSGSTIKPVVAAAALQEKIISENTSFLSVGGIYIGQWF
                     FPDWKAGGHGLTDVRKALAESVNTFFYYIGGGYQDFVGLGVDRLVTYMRLFGFGEKTG
                     IDLNGESAGFVPTPSWKEETKNEAWYIGDTYHIAIGQGDILVTPLQVANFTAAIANGG
                     TLYRPSLVQSLLAENNQKIKDIAPEIIRRDFIDPYNLQVVREGMRQTVTAGSARSLRV
                     LPVSVAGKTGTAQWSSTKENHAWFTGFAPYENPEIVITVLVEEGREGSEVSVPIAQEI
                     LNWYFSQPENIH"
     gene            8872..9558
                     /locus_tag="PKK65_02675"
     CDS             8872..9558
                     /locus_tag="PKK65_02675"
                     /inference="COORDINATES: protein motif:HMM:NF013768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81615.1"
                     /translation="MDLSKRQQLILHTLLKEYVKSAQPVASGILVEKYHLDISPATVR
                     NELMYLEEEGYIYQPHTSAGRVPTEKAYLLELATAGVKKRDLRQSDKDSLELALSSID
                     NLKPAAKVLAELSGNAVFWAFHKNNLYHTGLSNLFAQPEFKQTEIVYDVSQVIDRMEE
                     IIDNLFNDLPDGPKVFLGEDNPFGSFLGTVILKYRQDGVSGLVGILGPIRMDYNRNLS
                     LLSYLYEYFK"
     gene            9564..10595
                     /gene="grpE"
                     /locus_tag="PKK65_02680"
     CDS             9564..10595
                     /gene="grpE"
                     /locus_tag="PKK65_02680"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNU81616.1"
                     /translation="MENLKPGIYRHYKGNEYELLFIATQTETGESLAVYRSLVDNKIW
                     ARPLAMWLGKVTVEGKEKPRFTWVREACPRYPEPVVGSIIYNDSGEILLIKNPQWTNW
                     SIPGGHIKWGEKMEEALRRKIEEQTSLQIDKIKFITAADGIKLPYFLKDKHFIFLNFF
                     AHLAGGEPQLSDKMTEYVWVKPETALKEFSVAPFVVDLLAAFIRRQSGSDSDNDFEGK
                     YKRALADYQNLLKRSVKEKEEFVRFAIGDFLHDIIPVYDHLKMSLSALPENEKESAWV
                     KGVEYVLKQFKEILSARGVEEIKTKGQKFDHNLMEALEGKGDKVVQEVMPGYTLNGRV
                     IRAAKVIVG"
     gene            10685..11128
                     /locus_tag="PKK65_02685"
     CDS             10685..11128
                     /locus_tag="PKK65_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNU81617.1"
                     /translation="MSLIKWNPFLSDSFGFEDMDKMFGEILPAVRSNQFGFTPALDMY
                     EDKNNIVVETQLAGIDPEKVSISIENDVLCIKGESERQSEVDEKNYYRKEIRRGSFYR
                     SVPLPTKVDGEKATAVNEDGILKISIPKAPEAQPKTIKIQSSKKK"
     gene            11238..13148
                     /gene="dnaK"
                     /locus_tag="PKK65_02690"
     CDS             11238..13148
                     /gene="dnaK"
                     /locus_tag="PKK65_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNU81618.1"
                     /translation="MGKILGIDLGTTNSAMAIMDGGVPKILENIEGNRTTPSIVAISK
                     NGERLVGAAAKRQAVINPENTVYAVKRLIGRHFNDEEVQRDLSTMPYKIVQAGEGVKV
                     KMGGKDYSPQEVSAMILSKLKADAEAKIGEKITEAIITVPAYFDDSQRQATKDAGLIA
                     GLDVKRIINEPTAAALAYGFDKKQGQKIVVYDLGGGTFDVSVLDISTDTVEVKATNGN
                     THLGGEDFDKIIIDWIISEFKKDQGIDLSKDTLALQRIKESAEKAKIELSTAMETEIN
                     QPFITTDQNGPKHLVMKLSRAKLEVLVGDLVAKTIEPCKKAIADSGFSLSDINEVIMV
                     GGMTRMPLVQQKVKEFFGKEPNLNVNPDEVVAMGAAVQAGVLQGDVKDVLLLDVTPLT
                     LGIETLGGVATPLIERNTTIPTSKSQIFSTAADGQTSVEIHVVQGERPMAADNKSLGR
                     FILDGIPSAPRGIPQVEVKFDLDANGILNVSATDKATGKQQNITITASSGLSKEEIEK
                     MKKEAEAHAEEDKKKKEQVEIKNQAEAVIFTIEKMIKESGDKMKAEDKTELEAKLESL
                     KKTKEGEDFAAIQKSMEELNTVAQRIGVSMYQNQPGASSANQGNAANSAESSNTAKEG
                     DNVVEGEVEDKK"
     gene            13232..14311
                     /gene="dnaJ"
                     /locus_tag="PKK65_02695"
     CDS             13232..14311
                     /gene="dnaJ"
                     /locus_tag="PKK65_02695"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNU81619.1"
                     /translation="MSKDYYKDLGVDKNASAEEIKAAFRKKAHQYHPDKGGDEAKFKE
                     INEAYQVLGNAEKRKQYDQFGSSFQGDQAGGFSGFGGRGFGGFEGVNINIDDLGDIFG
                     GFGDIFGGFGGNRGRRGPEQGRNLEMKLVVDFLDAIHGTETEISFPHLKTCRVCHGSG
                     HATGAKIENCPTCGGKGTISRIQRTILGAIQTQTVCPDCRGEGKKAGASCSECQGKGR
                     IKQTEKVKVKIPAGINDGESIRLAGYGDAGEKGMPAGDLFLRIKVTPHSKFKREGDDI
                     YSEEEISMVQATLGDTIEVKTVHGAVKLKIPEGTQPQTVFRLKDKGAPRLRGRGQGDH
                     FVKVKVLIPKNLSKAQRQLLADLKI"
     gene            14391..14549
                     /locus_tag="PKK65_02700"
     CDS             14391..14549
                     /locus_tag="PKK65_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81620.1"
                     /translation="MFKCEWLKKLLGIKKDGCGHSEQAQTETPAPAAPITDAASTDLD
                     KQKEASLK"
     gene            14607..15458
                     /locus_tag="PKK65_02705"
     CDS             14607..15458
                     /locus_tag="PKK65_02705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNU81621.1"
                     /translation="MFIDTHAHINFHDFKDDADEVIRRSLDNDTWMVLVGSEYKTSNR
                     ALNYANHYQHGVYAAVGLHPIHLEEQTVANNDENGDYSFMTRAEEFNYDTYEKLAKFE
                     KVVAIGEIGLDYYHLDPTKNVNEAKRRQQEVFLKQLVLARNLDLPAIIHCRQAHDDLL
                     SILQDFRKVYADFIPSDRPWGVIHCFSGDEDLAWKYFSLGLIISFTGLITFSKQWDDV
                     LRKVPLHKFMIETDSPYMTPEPYRGRRNEPLLVQYVAGRVAEIRNSSVEKIAAATTET
                     ARQFFRI"
     gene            15573..15860
                     /locus_tag="PKK65_02710"
     CDS             15573..15860
                     /locus_tag="PKK65_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81622.1"
                     /translation="MDKHSLRQLIMSSAAYSLSSILGPLLLLGLPAYFIDQYLETKPL
                     VTLIAVFISFLITNVLLFKKVTKINRMMAKNFPPRISMDDDKKGNSVVKNE"
     gene            15863..16603
                     /locus_tag="PKK65_02715"
     CDS             15863..16603
                     /locus_tag="PKK65_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FoF1 ATP synthase subunit a"
                     /protein_id="HNU81623.1"
                     /translation="MQLDISLAPETLFHIASIPISNTFFWSWLIFAFLIVGSLFLRRS
                     LKMRPGHFQNIIEMLVGGAFDFVESMIGDKKRALRVFPLVFTMFFFIFFCNLLTFIPG
                     QAAVTIKTAGGVVPLFRAIISDYGLVFVMTMVSVIVTQIVAIASMGPLRYVGKFFNFT
                     NPLNFFLGLMDLVSELAKVMSLSFRLFGNIFAGEVLGAVMLFLFPFFLPLPFTLLSLL
                     TAVVQPFVFSVLTLVFISMAIQPSEAEA"
     gene            16671..>16773
                     /locus_tag="PKK65_02720"
     CDS             16671..>16773
                     /locus_tag="PKK65_02720"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; subunit C is part of the
                     membrane proton channel F0; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP F0F1 synthase subunit C"
                     /protein_id="HNU81624.1"
                     /translation="MEAAKYIGAGLAMGLGAIGPAIGEGIAASKALEA"
BASE COUNT         4661 a         3017 c         3802 g         5293 t
ORIGIN      
        1 cagtagaaga aattgataat cttattagta atattaaagg attattaaaa gagggaggga
       61 actgtcgtat aactccaatt agcactcaaa acgaaaaagc taaacaggca atgctatata
      121 atttaaaaca aattgtcgac tctaaagaat acaatataca tgccaataaa gatactttaa
      181 ttattcataa attattcata aatagaaatt agtcacatcg ggtttgatta aaactatatt
      241 tacaattatg accataaaaa ataaaccgga actattggcg ccagccggta atttagaaaa
      301 attaaagacc gctttagctc acggtgccga tgcggtctat tgtggtttgc cggatttttc
      361 tttacgtact cgcgttaatg atttttcttt ggaagatatt aaggaaggga ttgtctatgc
      421 ccacgcttta ggcaagaagg tttacattac tttaaatatt atcgctcgta ataatcatct
      481 ggccaagtta aaaagtcatg tcagaaaatt aaagacgctc aagccggacg ctatctttat
      541 cgccgatccc ggcgttttag aaatagttaa agcaatttgg ccgggggcac gcttgagtct
      601 aagtacgcag gctaattgca ccaatattgc ctctgctcgt ttctgggcga aacagggatt
      661 taagcgttta attttgtcgc gagaactggg aataaaagaa attgctgcta ttaaagctgg
      721 tcttaaaaat acggaaatag aagtcttcgt gcacggtgct ttgtgcagtt cctattctgg
      781 ccgctgcttc ttatctgatt attttagcgg ccgtagcgct aatctcggcg attgcgctca
      841 gccttgtcgc tgggaatatg aaattaaacc gtacgggcac gaaaaaagcc tgattgtggg
      901 cgaagacacg catggtactt attttttgaa ttctaaagat ttatgtttgc ttgaacgttt
      961 gccggaaata gtccatgccg gtgttaacgc tttgaaaata gaaggcagag ccaagagcgt
     1021 atattatcta gctaatgtgg tcggcgccta tcgtcgggcc ttagatttga ttgccgcggg
     1081 caaagccaag gcggttctta gaaaagagtt gtctttttta aagtcggaat taacagaaaa
     1141 attaaatcaa cgcggctata ccgaaggctt tatgtttaca agagaggaca atctgcagaa
     1201 ttatgagggt aattttccgg attctccttg ggaattttgc ggtcaagtta tttccagtct
     1261 taaacaagga aaacatttta tactactagt taaggttcac aatactttat tggctgacga
     1321 tgaattagaa attattgcgc ccgcttatcg tgtcaatcag ataaaagcgg cggcgatgcg
     1381 agacgccgaa acgggcgatg aaatttatga agctcatggc ggcggcggaa acagccacat
     1441 tttaattacg tccgccaaaa attggccagc ctatagcgtt ttgcgccgtc gtttaagaaa
     1501 aacaagctct taaagaaagc tggctacgtt ttttctgtct atatctgttt agttaaaata
     1561 tttggctatg actgagccga tgccgttgct cagtaaaagt acggctatgg ctatcactaa
     1621 atgttcgcct aagctttcta aacggctttg tccctggctt ttagccaaaa aataactaag
     1681 aagactaaga atgcttagtc cccagacgat gctgatgata atggccgtgg acaaggaaaa
     1741 aaatataacc gggacgacaa aggtaagggc aaagatgaat ttaaacaaga aagtaaagcc
     1801 ggtagcggcc aagacttctt tggctattgc ccccttttca gtttctttgg aaacatgaac
     1861 tccaagcgca tccgagaaag catcggcaag ggcgatggtt aaaatgccgc cgataacggc
     1921 tagttttgag ccagtggaag cgttcaggcc aatcatcaga cctagagtcg tgataaccgc
     1981 cgaggttaga ccgaagccta gtccggtcag catagattta ttcatatagt attattttac
     2041 cttgctatta taacaattat tagcatttat gtctttatgg cgggaaaaaa ataaaaaatt
     2101 tttatttttg cttcttggca gcttagtttt tttgtgttct ctcttttttt atcaaatcag
     2161 cggcaacggt ctggaggttc ggcctgatat caattctgaa agaaatgaaa aagataagaa
     2221 tgaaaaagat aaggaggaaa aaatatggcc agaattaccg atgcgcccac ctttggtttt
     2281 attattcggc ggtgatgtta tgctatcgcg cactgttaac gccaaaatgg ctgcctatga
     2341 tgattatgag tggccgtttg cacaaatatc tcaattaaca tctagcgccg atattaccat
     2401 ctttaatctt gagtctcctt ttttaaaaga tgccgattat aatgttcctt ccggctcttt
     2461 tatgtttaag gccgacccga aagctattat tggtttacag gcggcaggag tggatgtttt
     2521 gtctttagct aataatcata taatgaatat gggcgaccgg ggtcttgatg ataccttgtc
     2581 gattttacaa atggcgggaa taggtgctgt tggcgccggt caaaatgaaa aagcggcgcg
     2641 tcaaggatat ttgtggacag gtaagggctg gaaagtcgcc tttttatcct atgcctatcc
     2701 tgatgataat agtgttgccg gcgaaaaaaa gcctggcatc tctaccatgg atgtggacaa
     2761 tttgcgggaa gatatcgcgc gttggcgcca tcaagccgat ttagtgatcg ttttgatgca
     2821 cgccggcacg gaatatgttt cttctcccaa taaagagcaa gtaacttttg cccgggcggc
     2881 gattgatgcc ggcgctgatg ccgttatcgg ccatcaccct cattggccac agtcttggga
     2941 aatatatcag aataagccga ttttctattc tttaggcaat ttggtttttg atcagatgtg
     3001 gtcaagggaa accagccagg gattattggc ccgtctgact tttaacgata atttttctgg
     3061 gcaggcagaa ttgtggccaa ttattatcag tgattacgga caggcgcaat tatggccgga
     3121 agctgtgccg gaatctattt tttgggatgc ctatgattta ccgattccag ctgatttatc
     3181 ttggccagcc ctcggcgaca attaatttcc agaggtttta ataagggctc aaagcagttt
     3241 ttcttagata gaggagaaat tgtttttgtg atctttcggc attgcccgca tagttgtaat
     3301 atcgcttccg cttgtcgtcg gtagtatgat ggtaattctt ttttttctgg cagtcttttt
     3361 aatccgcgat gtgcaaaatt acggcgtcca ttttgccagt catcgcggta gtcgtagagg
     3421 tcgtcggcca attgtttagc tgccagcaga tagcgccccg cccgaaatag accctgggtt
     3481 tcggtttttt tccaacctaa aaacaaacta agatgtaacg gggcaattaa caaaaaaaga
     3541 gattttccgc ttggtgccaa tattcctcgc ggtattgttt ttggttgttt tacctcggaa
     3601 aaaagagcat tattttctct taacgataat tttaataaaa aattttttcc agttggttca
     3661 agtggcacgg ctaaactcag cgctaccgcg ctctcccagc aagctttaac caaaggcagg
     3721 tttattgttt tagtcgtccg atcgtcgagg aggtcgtctt gaagattggc ggcggtccag
     3781 cggtacaagg aagccgctcc taaatatatg gccgcatctt tttgtttggt ggatgggtgc
     3841 ggtggcagta tggaaagaac cagtgttgat attaatccct tgtggtcgtc tcttagtgtc
     3901 tccagccaaa acttctctag cgccccaacc agaggcttgg gccagtcttt ttttatattt
     3961 tccgccagtt gataaatttc ttttctttta ttcttcatat agggagacta accgtaaaag
     4021 cgctgccttt ccccagctgg ctatcaaccg cgatcgcgcc ttggaaatca ttttcaataa
     4081 tttctttgac tatgctaaga ccgagaccca cgtttttact gtcttcgggc ttggtggtaa
     4141 aaaagatatt aaaaatttta tgcagatgat cgggcgggat gccgatgccg ttgtctttaa
     4201 cggaaattaa aagtttattt gaatcagttt gttttttaat tgtgatttta attattccgg
     4261 ctgttggctt attttgcgcg caagcatcaa tagcattgga taaaagattc ataaatatct
     4321 gtccgaagcg gataggtcct cctttgatat aatagtcgcc ctccgcttca agttggatta
     4381 tggtttgtga cgctctcgct tttccttcca taattttttg gattttttct atttcttgcc
     4441 tgacggaaaa atatttttct tggttttgcc ggcgcagaca gcggttggca ctattgatta
     4501 attcgttgat tctggcggtg gcgctcagag cttgttgaac gtacacgttt gatttttcaa
     4561 tttcgcagtc gcctttcgtt tctatttgtt cgaggttaag gctaacaatg gtaagcggat
     4621 tgataatatc gtgaaaaata ctgcttgtca gttgtccaat actggctaaa gcctgcagtt
     4681 gttccagctt ttcccgctgc attattttca ctgctttagt tctttcggcg accttaattt
     4741 ctaattcttc gttaatttta ttgagctcct cttgtttatg ttttaaggac aaaaaagttt
     4801 gtttattttc ttttacgatc atccaggcaa gtccgaaaat gagtccgaga acaataacat
     4861 aagatagagc atcggctaat tgcatcggtt gttggcgcca atcgttattt actttgatta
     4921 tttggtttgt ttgcagccaa tttaagccga tgatggcgcc agcaaagatt gaagcaatag
     4981 ttaaggccgc tttcgtattt aaaaagatgc cggctaacat gattataagc accgtcatta
     5041 ataaagcggc cggcaagtcc gccccccaat aacttaaaca gaatattgtc ggcaaggtat
     5101 aggcggtaat cagcagccag gcgcttagat tcctataacc ttttttaaac agaatttgga
     5161 gaataataaa aaatgccaat agtccgagcg ttgcgcttaa ggggagtcct tgattatcgg
     5221 gattaataat aaaatcatgc aagcggataa gattaagtag aaacatgatg acgattgaga
     5281 tggctaaact tttttctagc tttttttctg ctggtgatgt tgattttatt gatttcagcc
     5341 aggctgccca tattttttta aacttcataa aaataaattt tgttgatatt aatttcggtt
     5401 cttcgcaagg ggttttccga aaattataaa agcgccttcc tttaaaagac aggaaggcgc
     5461 ttaggtttaa ctgcgccaag gggtgccggg attatcgata ttttccggtt tttgaccagt
     5521 taagtgtgaa aattttttca tataaggata ttttcgtttt ttgtaaagcg gctcgaccgc
     5581 ggactttatt tgtccccgct cttataatta tagcgagcgg agtctaaatt ctatttaaca
     5641 tttcagatag atgtgtccaa tttatatccg cgtccaataa tgctgtgaat taacttaggt
     5701 tgatggttgt ctattttccg tcttagcctg aggatgtgcg tctccacggt attagaaaag
     5761 ggatcgacat cgccactcca gacatgttcc aataaagatt cccgagaaac tatttttccg
     5821 gcattaagaa gaagatgttg gagtaaagaa aactccttgt tagttaagcg tatatcttta
     5881 ttagctcttc tgactttaaa tgattttggc tccagttcta agtctgcaaa aatcagtttt
     5941 tcttctttaa ttgtttgcgg tctccttagt agcgccctga ttctggctag cagctcttct
     6001 ccggagaagg gtttgcttag ataatcatcg gccccgatat ctaaaatatt aattttatca
     6061 attatacttt ggcggacgct aagcatgatg ataggtacat tgcgtccgtc gtccctaatt
     6121 tctttaataa tactgccacc atcgagttta ggcaaaacat agtcagtgat aattaagtca
     6181 taagcttgag ttcgggccat aaatgagcct ttttcgccgt cattggcaat atcgacggca
     6241 aagccggcct tcttgaggag agagcctagt aattcggcta aatttttttc atcttcaata
     6301 ataagtaagc gcatatattt attttaccat aaatttatta aaagctgaca gtctgccgag
     6361 atctgtcagc ttgaggactt gtctgttcct attaatcgca gaagatatct tctaaatcgt
     6421 agcgtccgta aggaagtttg gtcctgtgtc ggcatttttt atcaggtttt gccagacaga
     6481 gttttaattt tccttttctt ccttgatggg ggcaagttgt ggcggaacat tttggccagg
     6541 gaaggtactt tttcatgatt tttatttttt ataatgagct ttttctatta tatcaattgc
     6601 tcgataacat tcagttaaca tttttcgggt ttgccctttt ttaggaatca ggctagaatt
     6661 aaaatatggc ggtattgaaa aaaaaacgtt tttttctggg agattttagg tcatctcgag
     6721 gaaagggaaa cgaccctttt gtgataaaag agggtaattt taaatttggc cagttgaaag
     6781 attcagccta ttatactgat tggactgaac actcattttt atctgatagt cgcgacaagg
     6841 aagtggttgg gaggactttt gatgttaaaa agattctgcc gataaaaata ataattttgt
     6901 tatttattct tattttagct ggccggactg cttggctgca attaataaga gggtctcatt
     6961 attttggttt agctgagggc aatcgtctgc gcttagaaaa tattgaaccg aaaagaggaa
     7021 ttatttatga tcgaaagaga cgcgccttgg ttcgcaatac cgctaatttt gtcctatctt
     7081 taagaccgat agatttgcct agagatgaac tagctagaga tgaattgcta cggcgtatca
     7141 gctttataat cgacgatatg cccgcggagc ccactttaaa tgccaacttc gatttagtgt
     7201 ctgacggacc ctcttttcaa ctcatgaaaa acgctatcgc tggcatttct cctttttccc
     7261 ttgaggctta tcagccagtt tttattgccg ataatattgg ttatgagcaa gccttgcatc
     7321 ttcttgcttc cattgattct ttacccgggg tgattattga tactaaaatt aggcgggaat
     7381 atccttttac cgcggatcag gaagacggca ctccttcaga tttatccagt ctttcccata
     7441 ttttaggtta taccggtaag attactcttg aggctcttaa ccgtcttggc tctcaatact
     7501 ctttagttga ttatgttggc aagaccggtt tggaatattc ttgggagaaa gaattaaaag
     7561 gaattaacgg tcataaaaat attgaagttg atgctttcgg cagacgtaag aaggtggtga
     7621 gcgaattaga acctgttgct ggttataatc taaaattgtc tttagattta gatttacagc
     7681 gccaggcaga aattgttacc aagagttggt tattaaaaac aaaaacgtcc agggcggcgg
     7741 tgattgcgat ggatcctcgc aacggacaga ttttggcttt ggtgagccta cctgcttata
     7801 ataataatct ttttgcccgc ggtcttagcc aagaagaata tgatgttttt cttaatgatg
     7861 aaaatcatcc gcttttcaat cgtgcgatta gcggagagct tccttccggt tcgactatta
     7921 agccggtagt cgccgccgcc gctttacagg aaaagattat ttctgaaaat accagttttc
     7981 ttagtgtcgg tggtatttat ataggtcagt ggttttttcc tgattggaag gctggtggtc
     8041 atggattaac cgatgtgcgc aaagccttgg cggaatcagt caatactttt ttctattata
     8101 tcgggggcgg ttatcaggat tttgtcggtt tgggcgttga ccgattggtt acatatatgc
     8161 gcttatttgg ttttggagaa aaaactggta tcgacttaaa tggagagtct gccggatttg
     8221 tgccgacacc ttcctggaaa gaagagacta aaaatgaagc ttggtatatc ggcgatactt
     8281 atcatattgc catcggtcaa ggcgatatct tggtaacacc gcttcaagtg gctaatttta
     8341 ccgccgctat cgctaatggc ggtactcttt accgccccag tttggttcaa agcttgttgg
     8401 ctgaaaataa tcaaaaaata aaggatatcg ctccggaaat tattcgtcgc gattttatcg
     8461 atccctataa tttacaggta gtacgggagg gtatgcgtca gacggttacc gccggtagtg
     8521 ctcgcagttt aagggttttg ccggtaagtg tcgccggcaa gaccggcacc gcccagtggt
     8581 cgagtacgaa agaaaatcac gcttggttta ccggctttgc tccttatgaa aatccggaaa
     8641 tcgtgattac ggttttggtt gaagaggggc gagagggtag tgaggtttct gtgccgattg
     8701 cgcaggaaat tcttaattgg tatttttctc aaccagaaaa tattcattaa tgaaaaggtt
     8761 gtagtaataa ttttatatta atattagcca atttaatatt attattagca ctctattgac
     8821 ttgagtgcta atttttttta taatattatt agtaatctaa attaaggttt tatggattta
     8881 agtaaacgtc aacaattaat cctacacacc ttgttaaaag agtacgtgaa aagtgctcaa
     8941 cctgtcgcct ccggtatttt ggtggaaaaa tatcatttag acatttctcc ggccaccgtc
     9001 cgcaatgagt taatgtattt agaggaagag ggttatattt atcagcccca tacttcggcg
     9061 ggaagagtgc cgaccgagaa ggcctattta ttggaattag ctactgctgg cgtcaagaag
     9121 cgcgatttgc gccagagtga taaggacagt ttagaattgg ctttatcctc aattgataat
     9181 ttaaaaccgg cggctaaagt tttagcggag ttatctggca atgctgtttt ttgggctttt
     9241 cataagaata atctctatca taccggtcta tctaatcttt ttgctcaacc tgaatttaag
     9301 cagacggaaa ttgtttacga tgtgtctcaa gtaatcgacc ggatggaaga aattattgat
     9361 aatcttttta atgatttgcc ggacggtccg aaagtgtttt tgggcgaaga caatcctttc
     9421 ggttctttct taggtacggt gattttaaaa taccgccaag atggcgtttc cggcttggtg
     9481 ggtattttag gcccaatacg tatggactat aaccgcaatc tctcacttct ttcttatttg
     9541 tatgaatatt ttaaataata aatatggaaa atctaaaacc aggaatttat cgacactata
     9601 aaggcaatga atatgaatta ttatttattg ctacccagac tgaaaccgga gaatctttgg
     9661 ctgtctatcg ttctcttgtt gataataaaa tttgggctag acctttagcg atgtggctgg
     9721 gcaaggtgac ggtcgaaggc aaggaaaagc cccgtttcac ttgggtaaga gaagcgtgtc
     9781 ctcgctatcc ggagccggtg gtcggttcta ttatctataa tgactcgggc gagattttgc
     9841 tgattaaaaa tcctcaatgg accaattggt ctatccccgg tggacatatt aagtgggggg
     9901 aaaaaatgga agaggcttta cgccggaaaa tagaagaaca gacttcttta cagattgata
     9961 agattaaatt tattacagct gctgacggta tcaagcttcc atatttttta aaggataagc
    10021 attttatttt tttgaatttt tttgcccatc tggccggagg cgagcctcaa ttatccgata
    10081 agatgaccga atatgtttgg gttaaaccgg aaacggcttt gaaagaattt tcagtggcac
    10141 ctttcgttgt cgatttattg gcggcgttta tccgcagaca atcaggttca gactctgata
    10201 atgattttga aggtaagtat aaacgcgcct tggctgatta tcagaacctt ttgaaacggt
    10261 ccgtgaaaga aaaagaggag tttgtccgct ttgccatcgg tgatttttta catgacatca
    10321 ttcccgtcta tgaccactta aagatgtctt tatctgcttt accggagaat gaaaaagaaa
    10381 gcgcttgggt aaagggggta gaatatgttt taaaacagtt taaagaaatc ttgtccgctc
    10441 gcggcgtaga ggagattaag actaaaggac agaaatttga tcataatctg atggaggctt
    10501 tagaaggtaa aggcgataaa gtggtacagg aagttatgcc tggctatacg ctcaatggtc
    10561 gtgttatccg cgccgccaaa gtaattgttg gttaattaat cattattatt aaatttttta
    10621 gtttttctct ttatctacca tttaattata ttatttggtt ttaaagagaa aaagatataa
    10681 atttatgtct ttaataaaat ggaatccctt tttaagcgac agcttcggtt ttgaagacat
    10741 ggataaaatg ttcggcgaaa tattgccggc cgtacgcagc aatcaattcg gcttcacacc
    10801 ggcgcttgat atgtatgaag ataaaaataa tatcgtcgtc gagacgcaat tagccggtat
    10861 tgatccggaa aaggtaagta tttcaattga aaatgatgtt ttgtgcatta agggcgagag
    10921 cgaacgtcag agcgaagttg acgaaaagaa ttattatcgc aaagaaattc gccgcggctc
    10981 tttttatcgt tctgttcctc tgccaactaa ggttgatggc gagaaggcga cagcggtcaa
    11041 tgaggatgga attttaaaaa tcagcattcc taaggcgccg gaagcccagc cgaagacaat
    11101 taaaattcaa agcagcaaga agaaataatt tttatttgtc cgtgttcggc gcctttgata
    11161 aaaggcgtcg agacgggtga gtaaaagtga taaaaataaa attaattaat aatttcgtaa
    11221 tctaactatt aaaaaatatg ggaaaaattt taggaattga cttaggcacg actaattccg
    11281 ccatggcgat catggatggt ggcgtcccta agattttaga aaatattgaa ggcaatcgca
    11341 ccactccttc aattgtcgcc atttccaaaa atggtgagcg tttagttggt gccgccgcta
    11401 aacgtcaggc cgttattaat ccagaaaata ccgtttatgc cgtgaagcgt ttaattggtc
    11461 gacacttcaa tgatgaagaa gtccaacgcg atttaagcac gatgccgtat aagattgtgc
    11521 aggcgggaga gggtgtcaag gtgaagatgg gaggcaaaga ttattctcct caagaagttt
    11581 cggccatgat cctgtctaag cttaaagccg acgcggaagc aaaaatcggt gagaaaatta
    11641 ctgaagctat cattaccgtc cccgcttatt ttgatgactc tcagcgccag gcgactaaag
    11701 atgctgggct tattgctggt ttggacgtca agcgtatcat taatgaaccg acagccgccg
    11761 ccttggctta cggctttgat aagaaacagg ggcagaagat tgttgtctat gatttgggtg
    11821 gcggaacttt cgatgtctct gtgcttgata tttctactga tacggtggag gttaaagcta
    11881 caaacggtaa tactcattta ggcggagaag attttgataa gattattatt gattggatta
    11941 tctctgaatt taaaaaagat cagggtattg atttgtctaa agatacttta gctttgcagc
    12001 ggattaaaga atcggcggaa aaagctaaaa tagaattatc tacggcaatg gaaaccgaaa
    12061 tcaatcagcc ttttattacc actgatcaga atgggcctaa acatttagtt atgaagctaa
    12121 gtcgcgccaa actcgaagtt ttagtgggcg acttggtggc gaagacaatt gagccttgta
    12181 agaaagcgat agctgattct ggtttcagct taagtgatat caatgaagta attatggtcg
    12241 gcggtatgac ccgtatgcct ttagtccagc aaaaggtaaa ggaatttttt ggcaaagaac
    12301 caaatttaaa cgtcaatcct gatgaagtcg tggcgatggg cgcggctgtt caagccggtg
    12361 ttcttcaggg cgacgttaaa gatgtgctct tgcttgacgt cacccctctt accttgggga
    12421 ttgaaacttt aggtggagtt gctacaccgt taattgaacg caataccact atccctactt
    12481 ctaaatcgca gattttttcc accgcggccg acggtcagac tagtgtggaa attcatgttg
    12541 ttcaaggtga gcggccgatg gcggctgaca ataaatctct gggacgtttt atcctcgatg
    12601 gtattccttc agctccccgc gggatacctc aagttgaggt aaaatttgat ttggatgcca
    12661 acggcatctt aaatgtgagc gcgactgata aagccaccgg caagcagcag aatattacga
    12721 ttaccgcctc ttccggcttg tccaaagaag aaattgaaaa gatgaagaaa gaagcggaag
    12781 ctcacgctga agaagataag aagaagaaag aacaagtgga gattaaaaat caggctgaag
    12841 cggttatttt tactattgaa aaaatgatta aggagtcggg tgataaaatg aaggctgagg
    12901 ataagactga gcttgaagct aagcttgaat ctttgaaaaa aacgaaagaa ggcgaagatt
    12961 ttgcggctat tcagaaatcg atggaagaat taaatactgt cgcccagcgt attggcgttt
    13021 ccatgtatca aaatcagccg ggggcaagtt ctgctaatca gggcaatgcg gccaattccg
    13081 ccgaatccag taataccgct aaagagggtg acaatgtagt ggaaggtgaa gttgaagata
    13141 aaaaataatt ctgattcttt ttcagcaagc ctctcttttt ctggggaggc ttgctggctt
    13201 gagaataaga aattaagtct ggatatataa tatgtcaaaa gattattata aagatttggg
    13261 agtagataag aacgccagcg cggaggagat taaagctgct tttcgaaaaa aggcccacca
    13321 atatcatcct gataaaggtg gcgatgaagc caaatttaaa gagataaacg aagcgtatca
    13381 ggttttgggt aatgcagaaa aacgcaagca atatgatcag ttcggttctt cttttcaggg
    13441 tgaccaagcc ggtggtttta gtggttttgg cggtcggggt tttggcggtt ttgagggcgt
    13501 taatattaac atagatgacc ttggcgatat cttcggtggt tttggcgata tcttcggtgg
    13561 ttttggcggt aatcgcgggc gtcgggggcc agaacagggc aggaatttag aaatgaaatt
    13621 agtggttgat tttttagatg cgattcatgg tacggaaacg gaaattagtt ttcctcattt
    13681 aaagacttgt cgcgtttgcc atggcagtgg tcatgcaact ggagctaaaa ttgagaattg
    13741 tccaacttgt ggcggtaagg gaacgattag ccggatacag cggacgattt taggcgctat
    13801 tcaaacccaa actgtctgcc ctgattgtcg gggtgaaggt aaaaaagccg gcgcttcttg
    13861 ttcggaatgt caaggaaagg gaagaattaa gcaaacggaa aaggtaaaag ttaagattcc
    13921 ggccggtatt aacgatggag aaagcatccg tttagccgga tatggcgatg ccggcgaaaa
    13981 gggtatgccg gcgggtgact tgtttttgcg catcaaagtg acgcctcatt caaaatttaa
    14041 gagagagggg gatgatattt attctgagga agaaatttct atggttcagg ctactttggg
    14101 tgatactatt gaagtgaaga ccgtccacgg tgcggtaaaa ttaaagattc ctgaaggcac
    14161 tcaacctcaa actgtttttc gtttaaaaga taaaggcgcg cctcgccttc gtggtcgcgg
    14221 tcagggcgat cattttgtaa aagtgaaagt attgataccc aaaaatttaa gcaaagccca
    14281 gcgtcagtta ttagctgatt taaaaattta agcattgttt tgttttgact ttctaattgg
    14341 tggtgttata cttaatataa gaggaatttt ataaattaat cttttaattt atgtttaaat
    14401 gtgaatggct gaaaaaatta ttaggtataa aaaaagatgg ttgtggtcat tctgaacagg
    14461 cgcagactga gacgccggcg ccggcggcac ctattaccga cgccgcttct actgatttgg
    14521 ataaacagaa ggaagcttct ctaaagtaat attgtggaat aatctataaa aacgcgttta
    14581 acgcgttttt ttatcatttt tgttttatgt ttattgatac tcacgctcat ataaactttc
    14641 atgattttaa agatgatgct gatgaagtca ttcgtcgttc tttagataat gacacctgga
    14701 tggtgcttgt tggctctgag tataaaacct ctaatcgcgc tttgaattat gctaatcact
    14761 atcaacatgg tgtttatgcc gctgtcgggt tacaccccat ccatttagaa gagcaaacag
    14821 tggccaataa tgatgaaaac ggagattata gctttatgac acgggctgaa gagtttaact
    14881 atgatactta tgaaaaattg gctaagtttg agaaggtagt agcaattggt gaaattgggc
    14941 ttgattatta tcatctcgac ccgactaaaa atgtaaatga ggccaaaagg cgtcagcaag
    15001 aagttttttt aaaacagctt gtattagcgc gtaatcttga tttaccggca attatccatt
    15061 gtcgtcaggc tcatgatgat ctactgtcca ttcttcaaga ttttcgtaag gtttacgccg
    15121 attttattcc ttctgatcgt ccttgggggg tcattcattg tttttctgga gacgaggatt
    15181 tggcttggaa atatttttct ttggggttaa tcatttcttt taccggtttg attactttca
    15241 gtaagcagtg ggatgatgtt ctgaggaagg ttcctttgca taagtttatg attgaaaccg
    15301 actctcctta tatgacgcct gagccttatc gcggccgccg taatgagcct ctattggtgc
    15361 aatatgtagc gggaagggtg gcagaaatac gtaattccag tgtagagaag attgccgccg
    15421 cgacgacgga aacagctaga caattttttc gcatttgagc tttagttggt agtcagacgg
    15481 catttaaatt ttgatttttg ccagtcttga ctttttcgct aatcgggtgt atataacaag
    15541 tggttttcta aaaatttatc taatttaagc ctatggataa acattcttta aggcaattaa
    15601 taatgtctag cgccgcttat agcctttctt ctattctcgg tcccttattg ttgctaggtt
    15661 tacccgctta ctttatagat cagtatttag aaaccaagcc attggtgact ttaattgctg
    15721 tttttatttc ttttttaatc accaatgtcc ttttatttaa aaaagttact aaaatcaatc
    15781 ggatgatggc aaaaaatttt cctcctcgta tttctatgga tgatgataaa aaggggaatt
    15841 cagtcgtcaa gaacgaataa ttatgcagtt ggacatttct ttagctccgg agactttatt
    15901 tcacattgct tccattccaa tttctaacac ttttttttgg tcatggctaa tatttgcttt
    15961 tctaatagtc ggctctttat ttttacgccg aagtttgaaa atgcgtcctg gtcacttcca
    16021 aaatattatt gagatgttgg ttggcggggc ttttgatttt gttgaatcga tgataggcga
    16081 caaaaagaga gctttgcgcg tcttcccctt ggtttttacc atgttttttt tcattttttt
    16141 ctgtaacctt ttgaccttta ttcccggcca ggcggccgtt accattaaaa ccgccggtgg
    16201 cgtcgtccca ctttttagag cgattatttc tgattacggc ctcgtttttg ttatgacaat
    16261 ggtgtcagtg attgttactc agattgttgc tattgcttct atgggtccac tgcgctatgt
    16321 tggtaaattt tttaatttta ccaatccttt gaatttcttt ttaggtttga tggacttagt
    16381 gagcgaactg gcgaaggtaa tgtctttatc tttccgttta tttggtaata tttttgctgg
    16441 tgaagtgttg ggggcggtta tgctgttttt gttccctttc tttttaccct tacctttcac
    16501 tttattaagc ttattgacgg cagtcgttca gcctttcgtt ttttcagttt tgaccttggt
    16561 gtttatttct atggcgattc agccgtcaga agcggaggct taaaaggtag ctttaatatt
    16621 tattataaac cattaaataa ttcttaataa ttaatttcaa atttatggac atggaagcag
    16681 caaagtatat cggcgctggc ctcgctatgg gtttaggcgc tatcggccca gctattggtg
    16741 agggtatcgc cgcctcaaag gccttagagg caa
//
LOCUS       DAOQSA010000018        16242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_2674_length_16242_cov_9.981837, whole genome shotgun sequence.
ACCESSION   DAOQSA010000018 DAOQSA010000000
VERSION     DAOQSA010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 16242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16242
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2674_length_16242_cov_9.981837"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3270)
                     /locus_tag="PKK65_02725"
     rRNA            complement(<1..3270)
                     /locus_tag="PKK65_02725"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(3714..4769)
                     /locus_tag="PKK65_02730"
     CDS             complement(3714..4769)
                     /locus_tag="PKK65_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNU81625.1"
                     /translation="MEIRQILEEVARCQNSDLHLVFGMPPIARKDGQLVPFSDSNGVV
                     YTPLSDLELEQVAADLLSQEQRQKLKEKKDLDAAVSVGNARFRLNFAFDRGHLKVVAR
                     VISESRPTLDDLGMPASIADLLNSPQGLILVTGPTGCGKSTTLAAMINHINSQRNVHI
                     LTLEDPIEYIFTSDKSLISQRQLGEDMLSFAAGLKHALRQDPNVIMVGEMRDLETIAA
                     TVTLAETGHLVLATLHTYSAAQTIDRIIDVFPPHQQAQIRSQLSTILSAVISQRLLPR
                     ISGGRVAAREIMLRNSAIANLIRENKIPQIKSVIETNIQQGMTTMDRHLKQLYQAGEI
                     DRETALNYMEESGFLDA"
     gene            complement(4829..5521)
                     /gene="rnc"
                     /locus_tag="PKK65_02735"
     CDS             complement(4829..5521)
                     /gene="rnc"
                     /locus_tag="PKK65_02735"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNU81626.1"
                     /translation="MPELSNLEKTLGCKFKNQEILRQAMVHRSYLNEHPEFAGQHNER
                     LEFLGDAVLEIVVTEYLFLNYPDTPEGDLTNWRASLVNAKMLYLIAMELGLEEYLYLS
                     RGEAKDKNKKSRHYILANAVEALIGAIYIDQGLAIAKAFILDKIVVKLPEILKTQAYL
                     DPKSRFQEQAQDKRGITPRYEIISEEGPDHAKIFTVGLYLDNEFITSGSGSSKQEAQV
                     DAAAKGLKIFNW"
     gene            complement(5637..6161)
                     /gene="nusB"
                     /locus_tag="PKK65_02740"
     CDS             complement(5637..6161)
                     /gene="nusB"
                     /locus_tag="PKK65_02740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNU81627.1"
                     /translation="MSNRHLARTIALQTLFAWDFNGKNGQNTASLMRDNFVNFAPNFD
                     DGGFVNELVNGVIDNLPEIDSLIVKYATEWPLDQITTIDRNVLRLGIFELVYTAIPPR
                     VAINEAIEVAKSFGGDASGKFINGVLGAIYNDWPEEEKKKRDNHWGKAETASNGSSDG
                     IDASNPEPLEVATE"
     gene            complement(6165..6365)
                     /gene="rpmF"
                     /locus_tag="PKK65_02745"
     CDS             complement(6165..6365)
                     /gene="rpmF"
                     /locus_tag="PKK65_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNU81628.1"
                     /translation="MANPKKRKARSAVRTNRAHLALKKTSLNKCPKCGKAKKPHTVCN
                     FCGTYRGRQVLKIEAKAVKNKK"
     gene            complement(6456..8294)
                     /gene="ftsH"
                     /locus_tag="PKK65_02750"
     CDS             complement(6456..8294)
                     /gene="ftsH"
                     /locus_tag="PKK65_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNU81629.1"
                     /translation="MKNLAKNFLVFFIIFLAMAAIFSTFSNNSESVKVGIETMIQELN
                     QDQIKSLSVSGDTVKIELKDGSRQTVRKESGESLSSLLSNFQISPDKLNQVAINVQEP
                     SGWLYWLGELLPFILPLFFLGLFFFLMMRSAQGMNNKAMNFGQSNAKEFDLNNKNQVT
                     FKDVAGVKEAKEELYEVVEFLKYPKKFQDLGARIPRGVLLLGGPGTGKTLLARAVAGE
                     AKVPFFHMSGSEFVEMFVGVGASRVRSLFQKAKKHSPCIVFIDEIDAVGRRRGSGLGG
                     SHDEREQTLNQILVEMDGFEATQNIIVIAATNRPDVLDPALLRPGRFDRQVVIDRPDL
                     KDREEILKVHSQKKPLAKEVDLKRIAERTPGFSGADLANLLNEASILSARKNKKVVEM
                     EELFSAIEKVMIGPERKSRIISDKEKKITAYHEAGHALVAHFLVNSDPVQKVSIIARG
                     QAGGYTLKVPVEDRYFHSRTDFIDEIAVLLAGHLVEKEIFGEVTTGATSDLRQATLVA
                     RSLITDYGMSDLLGPRTFGQKEELIFLGREIHEQRDYSEKIAEQIDIEILNFINQASV
                     KAKEVLARHRDKLEKVAQALLTQETLEKEAFEALVGPAASLENKEA"
     gene            complement(8449..9600)
                     /locus_tag="PKK65_02755"
     CDS             complement(8449..9600)
                     /locus_tag="PKK65_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNU81630.1"
                     /translation="MTKETKQASSKFADLLEKDGVKVPQVGDVVKGTVVTASKSEVVL
                     DIDGVLMGIVRGPELYVEVEEYAHLKPGDQVEAAVLETENEEGHLEMSFRQVGQEKAW
                     QNLREAFKEKTTVKIRIIDANKGGLLARYCQINGFLPVSQLAPENYPRISGGDKSKIL
                     EKLKTFVGQDFEVNVITLNEEENKIIFSEKDVWNKKQKPALDRYHVGMVVDGRITAIT
                     NFGVFLNFDDGMEGLIHISEIAWQRIDSPNELFKVGDRIQAEIVSIDGSKIFLSSKKL
                     QKDPWQEASAKYSVGQVVKGVIVKVNPFGLFVKLDEEIHGLAHISQLKLGPKEKIGEL
                     FNAGEEKYFEIVSISPSEHRLGLKLAEDDGKKESVTEKKTSKDKKENDL"
     gene            complement(9771..10484)
                     /locus_tag="PKK65_02760"
     CDS             complement(9771..10484)
                     /locus_tag="PKK65_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81631.1"
                     /translation="MSMETMTGQPENLSNETEAMATPSNEEELGQQNKDLHEALAEAH
                     LSESRTPEKIAAVSEEIKESKSPEEIKMEIKRLQAEYEMKNKAWQSFTTISSADTDLG
                     SFGGSAESVAELENKFPGIGKVLEMIKLAEKKGDNGFKLFNLKTWKNFNKEPELGDVE
                     IKAIKNYLIHGGMGEFTNPNAEAVARKALDKFLSENSQAEGDVANIKQAIWGEHDDRY
                     YERAITDLNQQLEQMNEAK"
     gene            complement(10572..12383)
                     /locus_tag="PKK65_02765"
     CDS             complement(10572..12383)
                     /locus_tag="PKK65_02765"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HNU81632.1"
                     /translation="MITRYKSGITTGKDNVAKAPLKTDLSPRRRFPVRSRQVFRQNNS
                     GDYVKVIVLGGLEEVGRNMTLIECNREIIIIDMGLQFPEEDMPGIDYIIPNVSYLRDK
                     LDWIKGVIITHGHYDHIGGIPHIMGEIGNPPMFTGKLTAGLIRKRHQEYKTAPQLKIK
                     EIDDNSVIQLGASFRLEFTRVNHSIPDCFAVIVNTPLGTIMHTGDFKIDFTPVNDKPA
                     DLQRIAQIGNRGILMLMSDSTDATHAGYQLSESAIGEEIGKLIEKINGRIIIGTFASQ
                     ISRVQKILDLAKRYGRRVSLQGRSMNSNVEIAHQIGYLKFDPKILINDNELNRLPDNQ
                     VLIIGTGAQGEDSAFLSRVVNGEHRVVSLKQGDTVLFSSSVIPGNERSIQNLMDMVVR
                     QGAHIIHYRMLDIHAGGHAKAEDLKLIMRLIKPEYFMPIEANHYMLRAHAELAEQVGI
                     PSDKIFVADNGQVVLFQKDGRGHSVGQLTKERVPSDYVMVDGLGVGDVSEVVLRDRLM
                     MAGDGMIVIIATIDAKTGNIIGNPDIISRGFIYMKESRDLIEKTRMRVKKIVKDKNPM
                     TPADDDFIKNKIRNDVSQFLFAQTKRRPMVLPVVIKV"
     gene            complement(12506..14467)
                     /locus_tag="PKK65_02770"
     CDS             complement(12506..14467)
                     /locus_tag="PKK65_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNU81633.1"
                     /translation="MDQFSQKINSFFSRKKKNFSHFFVNVKDFIKEKFSTKGKKKAQT
                     ESDRNLVYSLAPTKIPRREQLRHLFKILNPKEKIILKLAFLVFLVSVFYLGFSFYQKH
                     LILIPASGGTYIEGLVGYPQSINPLYSSSRDVDADLSRLIYSRLFDYDENGRLMPNLA
                     STWKIEDGGKSYIISLREGVKWHNGEDLKIDDVIFTFNLIKNPEFRSSLRASLNGVEI
                     ERIDDHSVKFSLSEPYADFLSLLTFGIMPANIWENISPETASLSELNLKPIGSGPYKF
                     ESLIKSKGGEIKEYHLIANDDYYGPKPYIKEIIFKFFPDYVELVRALNGNEVDAASYI
                     PDDLKKDLLAKHSLVMHSLRLPQINSIFFNQEKNKFLGDIKIRQALAFAIDRDYLFNE
                     VLGESASRADGPIISPDFSYTPDGSFYNLDKDKAEQLLDESGFKRVDVSQDMIDNPEH
                     SPELSAIIAYASSTQIAATGYWRVALKDKEYQVLALKLSVPENGRMDVAESLKKDWEL
                     IGVKTIIVPVALASLNAEIIAGHNFEALLYGQVVGTEPDVAPFWHSSQIGGQGLNLSG
                     YNNAEVDTLLIEARQTADNINLRFSKYKKFQEIISKDLPVIFLYSPRYTYVQSKKVHG
                     FNGTAINEPCDRFAGFSHWYLKTKKHLSW"
     gene            complement(14507..14719)
                     /gene="secG"
                     /locus_tag="PKK65_02775"
     CDS             complement(14507..14719)
                     /gene="secG"
                     /locus_tag="PKK65_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNU81634.1"
                     /translation="MSLIQIAQIIVSILLIIVVLLQNRGAGLGSAFGGTGGVYLTKRG
                     LEKKLFIATIVLAVLFFGLSLVSVLL"
     gene            complement(14724..15071)
                     /gene="rplT"
                     /locus_tag="PKK65_02780"
     CDS             complement(14724..15071)
                     /gene="rplT"
                     /locus_tag="PKK65_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNU81635.1"
                     /translation="MPRVKRGVGHNKKRRTLLKQVKGYRWGRKNLIKLAKTARTKAGA
                     HAYRDRRKKKRDRRALWQVQINAFVREYNFSYSKLMGALKAAKIVVDRKILADLAINN
                     KKVLAALLSQLKK"
     gene            complement(15098..15295)
                     /locus_tag="PKK65_02785"
     CDS             complement(15098..15295)
                     /locus_tag="PKK65_02785"
                     /inference="COORDINATES: protein motif:HMM:NF013771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNU81636.1"
                     /translation="MPKIKTHRATAKRFRLTAANKLKKRKSGQDHFNSRETGNTKRAK
                     RLDINADKTLVKIIKTLTPYR"
     gene            complement(15343..15897)
                     /gene="infC"
                     /locus_tag="PKK65_02790"
     CDS             complement(15343..15897)
                     /gene="infC"
                     /locus_tag="PKK65_02790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNU81637.1"
                     /translation="MRIKFHRVEQKEKKFFKANSQIRSEQVFLIDENGENFGVIDTQE
                     AIARAKELDLDLVEVNPKAEPPVAKIADLGQLKYEHEKKLHRQKVMQKKVDTKVIRLS
                     FRISEHDFNFRLTQAEKFLEKDNKLKVELILRGRERQYPQKAREIVDNFVAKLRQKEN
                     LSIEIEQALTNQGGRFTIVLINKK"
BASE COUNT         4828 a         3669 c         2847 g         4898 t
ORIGIN      
        1 atattgaatg gtcggatcac taagttccgc tttcgcgact gcttgaccgg taagtctcgc
       61 agtaaagctg gcttgtgcct ttacactatc tcccgggttt ccattccggg atagccaacc
      121 tttgtaaacg cctccgttac attttgggag gcatccgccc cagacaaact acccgccaga
      181 aactgtcccc cgctatgcgg ggtaagaatt aaaaataggc aagggtggta tctcactgtt
      241 gcccgaaggc tcccacctat tctgaacata catattccta attcaatatc aagttgtagt
      301 aaagcttcac ggggtctttt cgtccaactg tggttaacga gcatctttac tcgtcttgca
      361 atttcaccga gtccttcgct gagacagtgc tcaagtggtt aagccattcg tgcaggtcgg
      421 aacttacccg acaaggaatt tcgctcttca cttacttaat atttctatta agaatggact
      481 ttatcttact cgtgatatga gtttaatgct tctaagataa acggcgctga ttctttattc
      541 tgcctgttca ttcttgaagc gatagtgatg atatgtttga tgccttcaat ggtcaaatgt
      601 tgaccggaat tcatcaactt tataatctcc ttaaacttga aataatcaaa tttcttttga
      661 gttaggagcg gatatttatc gaagtggggg atgacatatc tgtttatatg gtctaacgat
      721 cttattcgaa gttcctgacg cgtgtcatga tttactctga cgacgccagc tccgaaataa
      781 gtcttaagcg catataacac tttgatatct ttctcatgtt ggacaactct gaattccggt
      841 aatacttgaa aaccagattt catcgaatcg ttatgattaa tgccaacata aaatgtaccc
      901 tcaccatcaa caaatccgct aacccaatag ggattcacct tcatatttta gagtctcagt
      961 ttttaagtct ctgaggatta ttaatcctgc gcgtttgccc cgtgtatcaa gagattttta
     1021 ctggagtaga caccccgagt acttttgatc ttttgaggat tttcacgcat tttactgagt
     1081 attttagaca accatggctt tcgccatagc taggcaacga actttgttat ttatcaattt
     1141 ataatcgtcc cagtcttttg cggtctatat gataaaccgc aaaaccttag gacgattata
     1201 gttatcgccg gcgttcatcc gcgcttggat tcaaagcttt gccccgaagg gctaacctct
     1261 cctcttaacg ttcggacact ggccaggcat caccctctat acatcctctt gcgagttagc
     1321 agagagctgt gtttttgata aacagtccct tgagctcttt agctgcggcc ctgcttgcgc
     1381 agggcaggcc ttatctcgaa attacggccg ctgttttgcc gagttcctta gcgaaggttc
     1441 tctcgtacac cttaggcttc tcgcctcatc tacctgtgtc ggtttacggt acggatactg
     1501 tgcccttagc cctagaagtt tttctcgtcg gctctgccac ttgaattgac tccatcttgc
     1561 gacttcatct tctggctgtt tcttgaagct gttggcccgg atttgcctag gccgcctctt
     1621 gaaaccccca acggacatac cagtgcccgc tcaaacttta gaaccgcgtc cctccttcgg
     1681 aaaaacacag tagtgctgga atattaacca gctcttccat cggttattct aacactacat
     1741 tagatacacc ttaggatcga ctaaccctgg gtcgattctc gttgcccagg aaaccttaga
     1801 ttttcggtgg gcgggatttt aacccgcctt tctgctactc atgccaacat tctcacttct
     1861 ctccgctcca ccatgcctca cgacacgact tcaacgctga agagaatgct cctctaccac
     1921 tctacgataa atcatagagt ccacatcttc ggtaatatgc ttagccccgg taaattttcg
     1981 gcgcgaaaca acttgattag tgagctatta cgctatcttt aaaggatggc tgcttctaag
     2041 ccaacctcct aattgtcgcg gtcacaacac caccttttac acttagctta tatttaggga
     2101 ccttagattg tggtctgggc tgtttccctt ttgaccgatg aaccttagcg cccatggtct
     2161 gactcccgag ctgcacctac gggtattcgg agtttgattg aagacggtat cttgcgaccc
     2221 gccctcatcc agtgctctac cccccgcagt cagcactcga ggctagtcca aaaactattt
     2281 cgaggagaac cagctattgc cgagttcgat tacaatttct gcgctaacca cctctcatcc
     2341 ccgagtgttg cacggctcgt gggttcggtc cttcctctgc ctttcgacag agttcaacct
     2401 ggacatggtt agatcacccg gcttcgggtc ttgtccatgc gacgtgtact tcactttaac
     2461 gcactaattg cttaatgcgt aaaagtgaag tgcaatgcgg gctattaacc ctcgctttca
     2521 ctacacatac tctccggagg agattatgta agccacatag aacaaactcg ttggctcatt
     2581 cttcaatagg cacgccgtca ccgcgcaagc gcggctccga ctctttgtaa gtatatggtt
     2641 tcaggtacta tttcacctcc ctctcgggat gcttttcacc tttccctcac ggtactggtt
     2701 cactatcgat cataagaaat atttagcctt gggtcatagt cgacccggat tcatacggga
     2761 tttcacgagt cccgcactac ttaagaacaa gcacgacaga gaaataatcc ttttcgctta
     2821 cggggctatc acccactctg gctgagtttt ccaactcgct tctgctaaag attatttttt
     2881 atgactctgc tctgttcaat ctagagaaca gacatgctgt cttgcaaccc ctgcggaaca
     2941 tatggttcta gaaccttctg atttctcccc agcaagctgg atcaaaaccg cagttcggca
     3001 ggtttaggct cctccccgtt cgctcgccac tactaaggga atgacttttg ttttcttttc
     3061 ctccggctac taagatgttt cagtttgccg ggtcttccag cgccaccaaa cggcagcgct
     3121 tactccgtca acaacggagt gggtttcccc attcggagat ctgcgggtca aaggttgctt
     3181 gccacctcac cgcagcttat cgcaggctgc tacgtccttc ttcgttttct tatgtctagg
     3241 catccgccat acactcttta cgagattttc ccacactttt gttttcaaaa gtggacgcgt
     3301 tccttcgcga acgcgatttt ttaatttacc gcccggaaat tgcaaataaa ccgacggtaa
     3361 acggttcgct tttttgttaa aaagcgacaa aaaaatattt aactgttaaa gggcaattaa
     3421 atattcgttg ccattaccag tgaactgata agggcaagta aatatttaat ttagaaattt
     3481 attttagagg ttcaaaaaac cgcggctggc gcggtttaaa ataatcgcaa aaaaagcaaa
     3541 tatttataac cgcttaaatt tattgacttt aaaataaata ctcataggct gtttatgaaa
     3601 actgctacta attatagaac ttttaaaaat attgtcaagc cctataaaaa ggcttatttt
     3661 caaccttttt atcaatattt ataaccccca aaatagagac agtgcttcaa ttcttaagca
     3721 tcaagaaaac cactttcttc catataattt aaagccgtct cccgatcaat ctctccggcc
     3781 tgataaagct gctttagatg tctgtccata gtcgtcatac cctgctgtat atttgtttct
     3841 atcactgatt ttatctgagg aatcttattt tccctaatta agttggcgat cgcgctattt
     3901 ctcaacatta tttctctggc cgccacgcgc cccccggaaa ttctaggaag caatctttga
     3961 gaaataaccg ccgataaaat cgtagaaagc tgagaacgta tctgcgcctg ctgatgagga
     4021 ggaaaaacat ctataatacg atcaattgtc tgggcggcac tataagtatg aagagtggct
     4081 aaaactaagt gcccggtttc tgccaaggta acggtggcag cgattgtttc taaatccctc
     4141 atttcaccga ccatgataac attcgggtct tggcgtaggg cgtgtttcaa tccagcagcg
     4201 aaagacagca tgtcttcacc caactgacgt tgggaaatca agctcttgtc ggaagtaaaa
     4261 atatattcta tcggatcctc aagagttaaa atatggacgt ttctctggct attaatatga
     4321 ttaatcatgg cagcaagcgt tgttgacttg ccacaaccgg tcggaccggt aactaatatt
     4381 agtccttggg gtgaattcaa aagatcggca atcgaagcag gcatgcctaa atcatctaaa
     4441 gtgggccggc tttcactaat aacccttgcc accactttta aatgaccacg atcaaaagca
     4501 aaattaagcc taaaacgagc atttccgaca gaaaccgccg catccaaatc tttcttttct
     4561 ttaagtttct gccgctgctc ttgactaagt aaatcagccg ctacctgttc caattctaaa
     4621 tcagaaagag gggtataaac cactccgtta ctatcggaaa aaggcacaag ctgcccatct
     4681 ttacgcgcta tcggcggcat tccaaaaact aaatgcaaat cagaattttg acaacgagca
     4741 acttcttcta aaatttgtct tatctccata aaattaattt aaaattaaaa attattaatc
     4801 accccgcacc ggtttttaaa atctaaaact accaattaaa aatttttaag cctttggcgg
     4861 cggcgtcaac ttgcgcttct tgcttagagg aaccactacc gcttgttata aattcattgt
     4921 ccaaatacaa gccgacggtg aatattttag catggtcagg cccttcctcg ctgataattt
     4981 catagcgagg agtaataccg cgtttgtcct gagcttgttc ttgaaaacga gattttgggt
     5041 caagataggc ttgagttttt aaaatttcag gaagctttac cacgattttg tctaaaatga
     5101 acgctttagc tattgccaaa ccctgatcaa tataaatagc gccaattaaa gcctcgacag
     5161 cattagctaa aatatagtgg cgtgattttt tattcttatc cttagcttca ccgcgagaaa
     5221 gatagagata ttcttcaagc ccaagttcca tcgcaataag ataaagcatt ttagcgttaa
     5281 ctaaagaggc gcgccaatta gttaaatccc cctcaggcgt atcgggataa ttaagaaata
     5341 aatactccgt gactactatt tctaaaaccg catcacccaa gaattctaaa cgctcgttgt
     5401 gctggccggc aaactcaggg tgttcattta aatatgagcg gtgaaccatc gcctggcgca
     5461 gaatttcttg atttttaaat ttacaaccta aagtcttttc taaattagat aattcgggca
     5521 taaatttctc ggttacgagg cagaatcgag gcaccttttt aaactaggcg ccgccatact
     5581 gcttcataac tattaaataa aagcccgctt atgcggactt taaaagtgta gacaaattac
     5641 tccgtcgcca cctcaagtgg ttctggattg gaggcgtcta ttccgtcaga tgaaccattg
     5701 cttgctgtct ccgctttacc ccaatggtta tcacgcttct tcttttcttc ctcaggccaa
     5761 tcattataaa tcgctcccaa cacaccatta ataaatttac cggaagcgtc cccgccaaaa
     5821 ctcttagcaa cttcaatcgc ctcattaata gccactcgcg gaggaatagc cgtataaact
     5881 aattcgaaaa tacctaaacg caaaacattg cggtcaattg ttgtaatttg gtctaaaggc
     5941 cattcagtcg catacttaac aattaaagaa tcaatctcgg gcaaattatc aataacgcca
     6001 ttgactaatt cattaacaaa accgccatca tcaaaattag gcgcaaaatt aacaaaatta
     6061 tcgcgcatca aagaagcggt attctggcca tttttaccat tgaaatccca agcaaataaa
     6121 gtctgcaagg cgatagtacg tgctaaatgg cgattagaca tagactattt cttattttta
     6181 acagctttgg cttcaatttt taacacttga cgaccgcggt aagttccgca aaaattgcaa
     6241 acagtatgag gttttttagc ttttccacat ttcggacatt tatttaagct tgtttttttt
     6301 aaagccagat gagcccgatt ggtccgaaca gcgcttcttg cttttctttt tttaggatta
     6361 gccatatatt ttaaaattca aaaaatataa agaaatagac ttatttaatc tctattttta
     6421 gtataactaa aaaagaatat tagtcaaatt tcagcctatg cctccttatt ttccaaagaa
     6481 gctgctgggc caactaaagc ctcaaaggct tccttttcta aagtctcttg ggtgagcaaa
     6541 gcttgagcga ctttttccaa cttatcccga tggcgcgcca gtacctcctt tgccttaact
     6601 gaagcttgat tgataaaatt tagtatttca atatcaattt gctcggctat tttttcgcta
     6661 taatctctct gctcatgaat ctcccgtcct aagaaaatta attcttcttt ctggccaaaa
     6721 gtgcgaggac caaggagatc actcatgccg taatcagtaa tcagactcct ggcaactaaa
     6781 gtagcctgac gtaaatctga agtcgcaccg gtagtaacct ctccgaaaat ttctttttca
     6841 accagatgac cggctaaaag tacggcgatt tcatcgatga aatcagtgcg tgagtggaaa
     6901 taacggtctt caaccggaac ttttaaagtg tagcccccgg cttgaccgcg agcaataata
     6961 gacaccttct ggacggggtc gctgttaacc aaaaaatgag ccaccaaagc atgaccggct
     7021 tcatgatagg cggttatctt tttttccttg tcactaataa tccgactctt acgctctggg
     7081 ccaatcataa ctttctcaat cgcagaaaac agttcttcca tctcaactac ttttttgttc
     7141 tttctggcac ttaatataga agcctcattt aaaagattcg ccaaatcggc accggaaaaa
     7201 ccgggggtac gttcagcgat tctttttaaa tcaacttctt tagctaaagg tttcttttgg
     7261 gaatgcacct ttaaaatttc ttctctatct ttcaggtcgg gacgatctat aaccacttgg
     7321 cgatcaaaac gccccggacg caacaaggcc gggtctaaaa catcgggacg attagtggcg
     7381 gcaatcacaa taatattttg agtagcctca aagccgtcca tctccactaa aatttgattc
     7441 aatgtctgct ctctttcatc atgggaaccc cctaaaccag agccacggcg acggccaacg
     7501 gcatcgattt catcaataaa aacaatgcag ggagaatgct tcttcgcttt ctggaaaagt
     7561 gagcgcacgc gggaagcgcc aaccccgaca aacatctcca caaactcgga accggacata
     7621 tgaaagaaag gaactttagc ctcaccggca acagcgcgag ccaataaagt tttgccagta
     7681 ccgggaccgc ctaaaagtaa gacccctcta ggaatacgcg ctcccaaatc ttgaaatttc
     7741 tttggatatt ttaaaaactc taccacttca tataactctt ctttagcctc ctttactccg
     7801 gccacgtctt taaacgtaac ctgattttta ttattaaggt caaattcctt ggcattagat
     7861 tgtccaaaat tcatcgcctt attattcatg ccttgagcgc tgcgcatcat caagaaaaag
     7921 aaaagaccca aaaagaaaag cggtaaaata aaaggtaaaa gttcccccaa ccaatataac
     7981 caacccgacg gctcctgaac attaatggca acctgattta gtttatccgg tgaaatttga
     8041 aaattactta aaagagagga aaggctttca ccgctttcct tgcgaacagt ctgtcgactg
     8101 ccgtccttta gttcaatttt taccgtatct ccgctaacag acaaagattt tatttgatcc
     8161 tgattcaact cttgtatcat tgtctcaata ccaaccttaa cgctttcact attattcgaa
     8221 aaagtactaa aaatagcggc catcgctaaa aatattataa agaaaactaa gaaatttttt
     8281 gctaaattct tcatattttt actagaataa tattcccatt atataacggc aaagtctttt
     8341 tgacaatcga catcgacaaa aacgctcctc aaaaacgagg agccccttcg taaaagatta
     8401 gtatgtgtga ttcttttaat ttttatcagt aatagattct tcattagatt ataaatcgtt
     8461 ttctttctta tctttgcttg tttttttctc agttaccgat tcctttttac catcatcttc
     8521 agctaatttt aagcccaagc gatgttcaga tggagaaatg ctaacaatct caaaatattt
     8581 ttcttcgccg gcgttaaaca attcgccaat tttttctttt gggcctaact ttaactgact
     8641 aatatgggct aagccgtgaa tttcctcatc cagcttaaca aataatccga aagggttgac
     8701 tttaacaatt acccccttaa ccacttggcc gacgctatat ttagcgctgg cttcctgcca
     8761 aggatctttc tgtaattttt ttgagcttaa aaaaatttta ctgccgtcaa tgctgacaat
     8821 ttctgcctga atacgatcgc cgactttaaa taattcattg ggagaatcga tgcgctgcca
     8881 agcaatttct gaaatatgaa ttaaaccctc catgccgtcg tcaaaattta agaaaactcc
     8941 aaaattagtg atagcggtaa tgcgtccatc aacaaccatg ccgacgtgat aacgatctaa
     9001 agccggcttc tgcttcttat tccacacatc tttttcgcta aaaataatct tattttcttc
     9061 ttcatttaaa gtaataacat taacttcaaa atcttggccg acaaaagtct ttaatttttc
     9121 caaaatctta ctcttatcac caccgctaat gcgcggataa ttttcaggag ctaattggga
     9181 aaccggtaaa aatccgttta tctggcaata gcgagctaga agtccgcctt tattagcatc
     9241 aatgatgcgg attttaacgg tagttttctc tttaaaagcc tctcgtaaat tctgccaagc
     9301 cttctcttgt ccaacctgac ggaaagacat ctccagatgc ccctcttcgt tttccgtctc
     9361 taaaacagcc gcttcaactt gatcgcccgg cttaaggtga gcatattcct cgacttcaac
     9421 ataaagttcc ggtccgcgaa caatacccat taagacgccg tcgatatcta agacgacttc
     9481 ggattttgaa gcggtcacaa ccgtaccttt aaccacatca ccgacttgtg gcacttttac
     9541 tccgtctttt tctaataaat cagcaaactt acttgatgct tgtttggttt ctttagtcat
     9601 aaagataatc ttcatttatt cggaagcggt ctttcctctt ctaaaaacaa aaaaagatct
     9661 ttaaccgccc cttacgggac agcccttcgg ccacctcagg cactgcttta gagatagcgc
     9721 cttagaaccc gaaagaatta taataggtga aatatttaat taataaaagc ttatttggct
     9781 tcgttcattt gctccaactg ctgattcaaa tcggtaatcg ctctttcgta ataacgatcg
     9841 tcatgttctc cccaaatagc ttgtttaata ttagcaacat ccccttcggc ttgagaattt
     9901 tcagataaaa acttatctaa agctttacgg gcgactgcct cagcgttagg gttagtaaat
     9961 tcccccatac caccgtgaat aagataattt ttaatggctt taatctccac gtctcccaat
    10021 tcgggttcct tgttaaaatt tttccaagtt tttaaattaa aaagtttaaa tccattatca
    10081 cctttttttt cagctaattt aatcatttct aaaactttac ctataccggg aaacttattt
    10141 tctaattcgg ccacactttc ggcgctacca ccaaaagacc ctaaatcagt atcagcgcta
    10201 gaaatagtag taaagctttg ccaagcttta ttcttcattt catactccgc ctgcaatctt
    10261 ttaatttcca tcttaatctc ctccggggat ttagattctt taatttcttc agaaacggcc
    10321 gctatttttt caggagtcct actttcagag aggtgcgcct ccgccaaagc ttcgtgcaaa
    10381 tctttatttt gctgaccgag ctcctcttca tttgatggcg tagccatcgc ctcagtttca
    10441 tttgataaat tttcaggctg gcccgtcatg gtttccatac tcatatgatt ttaaataatt
    10501 gtatgactaa ttatataata attaagcaaa aaagtcaata gtatcgcaaa aacaaaataa
    10561 aattatagta attacacctt aatcactacc ggtaaaacca taggtcggcg ttttgtttgg
    10621 gcaaataaaa attgactaac atcgtttcta attttatttt taataaaatc atcatctgct
    10681 ggcgtcattg gatttttgtc tttaacaatc ttcttgaccc tcattctggt tttttcaatt
    10741 aaatcccgac tttccttcat ataaataaaa ccgcgcgaaa taatatcagg attaccgata
    10801 atattaccgg tcttagcgtc aatcgtggca ataatcacaa tcataccgtc accggccatc
    10861 attagacgat cacgcaagac aacttccgat acatcaccga caccaagacc atcaaccatg
    10921 acataatcag acggaaccct ttctttggtc agctgaccaa ctgaatgtcc gcggccgtct
    10981 ttttgaaaca aaactacttg accattatca gccacaaata ttttatccga aggaataccc
    11041 acctgttctg ctaattctgc gtgtgccctc aacatatagt gattagcttc aatgggcata
    11101 aaatattcag gtttaataag acgcattatt aattttaaat cttccgcttt ggcgtgtccg
    11161 ccagcatgaa tatcaagcat ccgataatga ataatgtgcg cgccttgacg aacaaccata
    11221 tccatcaagt tctggattga gcgttcattc ccaggaataa ccgaagaaga aaacaggaca
    11281 gtgtctcctt gttttaaact aacaacgcgg tgttcaccat ttaccacccg gcttaaaaaa
    11341 gcactgtcct ccccttgggc accggtgccg atgattaaaa cttgattatc cggcaaacgg
    11401 tttaattcat tgtcattaat taaaattttc ggatcaaact ttaaataacc tatttgatgg
    11461 gcaatttcga cattagaatt catgcttctc ccctgcaaac taacccgacg accgtagcgt
    11521 ttagccaaat ctaatatttt ctgcacacgg ctaatttgag aagcaaaagt gccgataata
    11581 atacgaccgt taattttttc aatcaatttg ccgatttctt caccgatggc cgattcagaa
    11641 agctgataac cagcatgagt agcatcggtc gagtcagaca tcaacattaa aataccgcga
    11701 ttgccaattt gagcgatgcg ctgtaaatct gcgggcttgt catttaccgg ggtaaaatca
    11761 attttaaagt cgccggtatg cataatagtg cctaaaggcg tattaacaat cacagcaaaa
    11821 caatcaggaa tagaatggtt gacgcgggta aattccaaac ggaaagaggc gccgagctga
    11881 atcaccgaat tgtcgtcaat ttctttaatt tttaactgag gtgcagtttt atattcttga
    11941 tggcgcttac gaatcaaacc ggccgtcaat ttaccagtaa acatcggcgg attaccaatc
    12001 tcccccataa tatgaggaat tccaccaata tgatcataat ggccatgagt aataattacg
    12061 ccttttatcc aatcaagttt atcgcgcaag taggagacat tgggaataat ataatcgatg
    12121 ccgggcatat cctcttccgg aaattgtagg cccatatcga taataataat ctcacggttg
    12181 cattcaatta gagtcatgtt tcgtccaacc tcttccagtc cgcccagaac aataacttta
    12241 acatagtcgc cagagttatt ctgacgaaaa acttgacgcg aacgaacagg aaatcggcgc
    12301 cttggcgaca aatctgtttt caagggcgct ttagcgacat tatctttgcc ggtagtaatt
    12361 ccggacttat aacgagtaat catgaaaatt tttctggaaa aacaaaaaaa tgaaaatcaa
    12421 aacaattctg atttttttgt ttccaaatta tgaagtatag agcatccgac tttaaaatta
    12481 gccggaaaaa ataaatagaa aataattacc aagaaagatg cttcttggtt tttaaatacc
    12541 aatggctaaa accagcaaaa cgatcacacg gttcattaat agccgttccg ttaaaaccgt
    12601 gaactttctt ggactgaaca taagtatatc ggggagaata taagaaaata accggcaaat
    12661 ccttactgat aatttcttga aattttttat acttagaaaa acgtaaatta atattatcag
    12721 ctgtctgtct cgcttcaatt aaaagcgtat cgacttcggc attattataa ccggaaagat
    12781 tcaagccctg accgccaatt tgtgaagaat gccaaaaagg agcaacatca ggctccgtgc
    12841 cgacaacttg tccatacaat aaagcttcaa aattatgacc agcaattatt tctgcgttta
    12901 agctggcaag cgctaccggc acaataattg ttttgacgcc gattaattcc cagtctttct
    12961 ttaaactctc agccacatcc atcctaccat tctcaggcac tgataatttc aaagctaaaa
    13021 cttgatattc tttgtcttta agggcgacgc gccaataacc ggtggcagca atttgagtgg
    13081 aactagcata ggcaataatg gctgataatt ctgggctatg ctcgggattg tcaatcatat
    13141 cttgactaac atctactcgc ttaaatccgc tttcatctaa aagctgctcg gctttatctt
    13201 tatctaaatt ataaaaagaa ccgtccggag tataggaaaa atcaggagag ataatagggc
    13261 cgtcggcacg actggcggat tcgcccaaaa cttcattaaa taagtaatcg cgatcaatag
    13321 caaaagccaa cgcctgacgt attttaatat cgcctaaaaa tttattcttc tcttgattga
    13381 aaaaaataga atttatttga ggcaagcgca aactatgcat caccaaagaa tgcttagcta
    13441 ataaatcttt ttttaaatca tcaggaatat aactggcggc atcaacctca ttaccgttta
    13501 aagcgcgcac caattccaca taatcaggaa aaaatttaaa gataatctcc ttgatataag
    13561 gtttaggccc ataatagtca tcattagcga tcagatgata ttctttaatt tcccccccct
    13621 tgctcttaat taaagattca aatttataag gaccggaacc aatcggtttg agatttaatt
    13681 ctgataaaga agcggtctca ggagaaatat tttcccaaat gtttgccggc atgatgccga
    13741 aagttaaaag acttaagaaa tcagcataag gctcgctgag agaaaatttg accgaatgat
    13801 cgtcaattct ttctatctct acgccattca ggctggcgcg caaggaagaa cgaaattctg
    13861 ggtttttaat caagttaaaa gtaaagataa catcgtcaat ctttaaatct tcaccgttat
    13921 gccacttcac tccttcacgt agagaaataa tataagattt cccgccgtct tcaattttcc
    13981 aagtagacgc taaattcggc attaaacggc cattttcatc ataatcaaac aaacgagaat
    14041 atattaagcg agataaatcg gcatcaacat cacggctact agaataaagg ggattgatgg
    14101 attgaggata accaactaag ccttcaatat aagtgccgcc actagcaggt attaaaataa
    14161 ggtgtttttg ataaaaagaa aagcctaaat agaaaacact gactaaaaaa acgagaaaag
    14221 ccaacttaag aataattttt tctttggggt ttaaaatttt aaaaagatgt cttaactgtt
    14281 cacgtctggg aatcttggtt ggcgctaagg aataaaccag atttctatca ctttctgtct
    14341 gcgctttttt tttcccttta gttgaaaatt tctccttaat aaaatcttta acattaacaa
    14401 aaaaatggga aaaatttttt ttctttctgg aaaaaaaact atttattttt tgagaaaatt
    14461 gatccacagg gtggcaatag acgaaagggg aagaataaaa atcggcttag agcaaaacac
    14521 taaccaaaga caagccgaag aaaagcaccg ccaagacaat ggtggcgatg aataattttt
    14581 tttcaagacc gcgtttcgtc aaataaacgc cgccggtacc accaaaagca ctgccaagac
    14641 cagcgccgcg gttttgaagg agaacgacga tgataagaag aatggaaaca ataatctgag
    14701 caatttgaat caaagacata agcttatttc ttaagctgag acaacaaggc cgctaaaact
    14761 ttcttattat taatagccaa atcagctaaa attttccgat caaccacaat cttggccgct
    14821 tttaaagcgc ccattaattt tgaataagaa aaattatact cgcgcacaaa agcattaatt
    14881 tgcacttgcc ataaagcccg ccgatcgcgt tttttcttgc gacgatcacg gtaagcgtga
    14941 gcaccggctt tagtacgagc agttttggct aatttaatta gatttttgcg accccaccga
    15001 tagcctttaa cttgctttaa cagtgtgcgg cgttttttat tgtggccaac tcctcttttt
    15061 actctgggca tatacgatta acttaaaaat ccgcatccta tcgatacggg gttaaggttt
    15121 taatgatttt tactaaagtc ttgtccgcgt taatatctaa acgcttagcg cgcttggtat
    15181 tgcctgtttc gcgagaatta aaatgatcct gaccggattt tcttttcttt aatttattag
    15241 cggcagttaa gcgaaaacgc ttagcggttg ctcggtgagt ctttattttc ggcataaatt
    15301 tgataaaaaa acaggcgaaa tcacctggat gataacaaga aattatttct tatttattaa
    15361 cactatagta aatcttccac cttgatttgt caaggcttgt tctatttcaa ttgataaatt
    15421 ttctttttgt ctaagtttag ccacaaaatt atccacaatt tctctagctt tctgcggata
    15481 ctgacgttca cgacctctta atatcaactc aacttttaat ttattgtctt tttctaaaaa
    15541 tttttctgct tgcgtgagac gaaaattaaa gtcatgttca ctaatacgga aagacagtct
    15601 aatcaccttg gtgtctactt ttttttgcat gaccttctgg cggtgaagtt tcttttcgtg
    15661 ttcatatttt agttgtccca aatctgcaat cttagccacc ggcggctcag ccttagggtt
    15721 tacctcaact aaatccaaat ccaattcctt cgctctcgca atagcctctt gagtatcaat
    15781 aacgccaaaa ttttcaccat tttcatcaat taaaaaaact tgttccgagc gaatctggga
    15841 attggcttta aaaaatttct tttctttctg ttcaacgcga tgaaatttaa tgcgcatggt
    15901 aatataagta ttatttatat agctatttat agcggaaaaa ttaaaaaaag tcaactaaat
    15961 aatcaagttg accaaatttt tgacaattaa aaatttcatt taataaaaag ctaaaactaa
    16021 aaatagaaga ctagctataa tcgctaacca tataaaagta aaaagaataa ttttgaaact
    16081 cccctttttg tccatcttaa ttcattatca ccggcctttc taaaatggcg ccgatagcag
    16141 ctgtcgatat aaccaataga agtaagataa ggtttatatt tatatagaga ccaaaagaat
    16201 ataaagaaaa aacagcccct ttttaggctg ttttgttgga gc
//
LOCUS       DAOQSA010000019        15994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_2733_length_15994_cov_9.070833, whole genome shotgun sequence.
ACCESSION   DAOQSA010000019 DAOQSA010000000
VERSION     DAOQSA010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15994
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2733_length_15994_cov_9.070833"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PKK65_02795"
     CDS             <1..372
                     /locus_tag="PKK65_02795"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908925.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HNU81638.1"
                     /translation="IILADKFCDIFDGFSIGSNDLTQLLLGIDRDSGGSLKIKGVSNE
                     KNEAVKTMIKNLIAVAKKRKRKVGICGQGPSDFPDFAEFLMAAGIDSISLIPDTVIPT
                     TMNLASKEKSLRRRGFLSFLF"
     gene            complement(443..1045)
                     /locus_tag="PKK65_02800"
     CDS             complement(443..1045)
                     /locus_tag="PKK65_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81639.1"
                     /translation="MKKFKISEDLVPLSRRQRTALTLIIACIFQFVLFFTPVLAAEAV
                     TLAQGDIKMDTTSKSEADPIVADLIKNANITVDTENVEVATDKLETNTPAIKVIRTST
                     HTITAYNSDPAQTDNTPCITANGFNVCKHGEEDTIAANFLQFGTKVRIPELYGDRIFV
                     VRDRMNKRYSDRIDIWMKEKTDARQFGVKVAKIEVVEFID"
     gene            1327..2187
                     /locus_tag="PKK65_02805"
     CDS             1327..2187
                     /locus_tag="PKK65_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81640.1"
                     /translation="MAVTIGINAQTRQQRKALEKVATEKAALEKELAELEISIATYVA
                     IDTLPTYSRKKEIDQALASGEMSIKQKAAYSDQSHKLGRKLAAMRQQLLVDTASFAYC
                     SRRINEIKFRLQKLNDYQTDLINNQLDEAVNQIPPQEMSSREAKRRHRGNIIKMETNK
                     AAFQEKLQAMSVSKKEAAPVIADPINGFFGRVINRSSHTVYNFVIYEKNSKAELKSFV
                     LEPGTSASNYFLPGEYACDVKAYGVIVAHYEFHVTADTHDVFGEELHWAVWQESEVRK
                     PMAKNITLNH"
     gene            complement(2244..2687)
                     /locus_tag="PKK65_02810"
     CDS             complement(2244..2687)
                     /locus_tag="PKK65_02810"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81641.1"
                     /translation="MKNIKIRLIIAVLAIILPMSVVSARTTTAKSLVETIDEPTQKML
                     GETGLNDFTLVQIIVAIIQVALSLLGIIFLIIIVFAGYSWMTAAGNEEAVKKAQDMIK
                     RAIIGLVIVLMAYAITYFIFNQLPFSGGGGIGTGNNSIPGGGATP"
     gene            complement(2690..3115)
                     /locus_tag="PKK65_02815"
     CDS             complement(2690..3115)
                     /locus_tag="PKK65_02815"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HNU81642.1"
                     /translation="MKLKSLKPIFTLLLLTAIMILPYFVFAQSTSTGSVNTSSSGNPL
                     TILENIASGENGPYTKADENSLLTIIGLIINGALGLLGVIFIALVILAGYNWMTAAGN
                     EEAVKKAQNTIQHALIGLIIVLSSWAIWNFILVNIIERL"
     gene            complement(3123..3533)
                     /locus_tag="PKK65_02820"
     CDS             complement(3123..3533)
                     /locus_tag="PKK65_02820"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81643.1"
                     /translation="MSARHKINIIIIALTLFIFTGALSVAAQEQTNANLKTAFDTSSS
                     SALGIVSGAGGAGYNNNVVLETVVGRIITGLFSLLGVIFIIFIIYGGYLYLTASGNEE
                     QTKKATSIITQNIVGLVIILGAYALTYFIIKFFI"
     gene            complement(3538..3933)
                     /locus_tag="PKK65_02825"
     CDS             complement(3538..3933)
                     /locus_tag="PKK65_02825"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HNU81644.1"
                     /translation="MRAKRLISLIFIFILSLGVFNLALAQNGNYDFSQNSGLSNTGNK
                     AGYKTTDTTINDYIAQIITVILSLVGVVFLGLAIYAGIIWMTASGSEEKVSKAKEMLT
                     ESIIGIIIVLAAYAISYFILKATSGSLIQ"
     gene            complement(3935..4327)
                     /locus_tag="PKK65_02830"
     CDS             complement(3935..4327)
                     /locus_tag="PKK65_02830"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81645.1"
                     /translation="MSRAQITKSVFSFLITSLIIAGFFSVLPVSADDYGLSQSANVVD
                     AYKTQAANPDNNFLSTRLGSIIGLVLSFVGVIFLILMIFAGLSWMTASGNQEKVNKAK
                     DLMINAVIGLVIVMAAYAITAFVGDKLT"
     gene            complement(4450..4830)
                     /locus_tag="PKK65_02835"
     CDS             complement(4450..4830)
                     /locus_tag="PKK65_02835"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HNU81646.1"
                     /translation="MSKKILKNLVVFAMLSLLALPIVTMAQNIDVGINEIEEGFGDNG
                     LGNTDPRTTIARLINVAMLFLGIIAVVIILVGGFKWMTAGGNEDKVSEAQKLMGAGVI
                     GLVIVLAAWGIASFILGRLISATT"
     gene            4930..6528
                     /locus_tag="PKK65_02840"
     CDS             4930..6528
                     /locus_tag="PKK65_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81647.1"
                     /translation="MNNKINKLSIVILGFLVFVAVALFFLSNSYSYSDVDLGWHLRIG
                     QDILKSGQVPVFNDYNYTLSGQKWVDHEWLLNAGMALLFNHGKFISLHLVFLLVLLVT
                     AFFVWRRNINLLGNKIVFKLGSAIWLFLGLLASRPHLGIRVQEFGLLGMAILFWIFSG
                     PSRSRHYFWYLPPIFWLWANIHGSFLLGLALLGAYCIYAFASPLFKHVNHWQLFRVDS
                     FDLKFKKQLLVIFTLSVLATLINPYGVDLYAFLGTYTHTTYMVYIREWQSQFVFPLFY
                     VQIFYLALAAVALIMLIKDRRRNLFIFSWWEAFLFVFFFILAIKSRRHFPLFVLASLP
                     FLSVAFQEFSAQIIGLAKRCRSLIWRALAIFLMFILVIYQAVNIPWFQNSIQDFCGRK
                     YPCAAVEYLRARPDLLNKRLFNSYDWGGYLLWAYPERQIFIDGRMPQAEYRHHTILEE
                     YLRFRSGSEDIGNLLNTYQIDLVLLKKSVSPLALRSWEKLFFQISEEELTAPDYLRLY
                     LDSSLDWQVVFEDNISIIYERQKN"
     gene            6509..7876
                     /locus_tag="PKK65_02845"
     CDS             6509..7876
                     /locus_tag="PKK65_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81648.1"
                     /translation="MNAKKINIWKILFWFALAVLAVFFHSDHFLNGDEGVTLNGAWNL
                     YNHRHLYFDFFSFLPPLSFYLIYWIWLILGVSFWSAKAFSIILLLAGAFGLFKISEHL
                     RSHPINYFVPFLFILISSWWWIINHNFYYLVFSIWSCYFLLLFLENNTRRHCLAAALL
                     ASASIATLQQKGLLFALASFLIILFFNKRQFGRRRFLAAALYVLGVAAPLVLLLFFWP
                     LSVLWQNLVIFPLFHYIEANRLPYYLLFLASISWLLFAWPFRSYDYRRWALLILSGVL
                     IISCYPLPDYYHLSMALVLLLPMLPDLLDKLNNPAGRCRRVLAISGLTWLWLWPVSHF
                     LAFFFNFSFTKDYPFLDYIRRECPGQYLQVGPFLPNVYLETGKLSATSFDILITGHQT
                     AEQFAQAAAQLEINQPVCAVTAYPASLSRFRHQLDNPVDNFIRANYQPVFTEGSITVW
                     KYRNQ"
     gene            complement(7947..8330)
                     /locus_tag="PKK65_02850"
     CDS             complement(7947..8330)
                     /locus_tag="PKK65_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81649.1"
                     /translation="MQNLLTWHFWFNLRPEPLLPIFVQIFLGFLILLLLASIFTGIKK
                     NRKSLYKIFWKKAYNFSIGNLIIGTAFFFFNYEQAAFLSARFWLAIWVIIMLWWLWPI
                     IKQYRMVPTKQKQLAEEQEFKKYIP"
     gene            complement(8336..8842)
                     /locus_tag="PKK65_02855"
     CDS             complement(8336..8842)
                     /locus_tag="PKK65_02855"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNU81650.1"
                     /translation="MNKIAITEGQRVAELGCGNFGYFVFPVARMVGARGRVFAVDILK
                     NTLEDIKKRALQENLQQIETVWSNLEIFKGTKIESASLDQALLINILHQSNKRVEILR
                     EAVRMLKNGSQLLVVEWKNSDSPFGPRSEQRVSKESLKEAAPKLGLNLKTEFEAGPYH
                     YGLIFYKV"
     gene            complement(8871..9548)
                     /locus_tag="PKK65_02860"
     CDS             complement(8871..9548)
                     /locus_tag="PKK65_02860"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNU81651.1"
                     /translation="MVEFNFKKLASEERSGEKLRAARLRQNIDIKNASEKLHIREEYL
                     LSLENEEYDRLPSGLYGRQFLKEYCRYLNLNYQDILKLSPFAEEAVSHNPFSQKILRR
                     HKFLIFPKIIRNALLILLFLICILYLSIYFFRLVSPPELILEYPDKNLVLQNPIINIQ
                     GKSEPETEIKINGTGIMSDQDGHFSQEIKLKQGLNNLTISAKKKYGRESVIQRQILVE
                     NTYEQSQ"
     gene            complement(9556..10008)
                     /locus_tag="PKK65_02865"
     CDS             complement(9556..10008)
                     /locus_tag="PKK65_02865"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81652.1"
                     /translation="MIKKALIGLAFIISFFLSPALPTLAQSISIVDTTSTTTEGVNQG
                     DTKYSTGDYTLDDIVMIAVRASRWILGIVGSLALLMFVYGGFMFLISSGSSDKISKAK
                     AIIVAAVVGLIIVFSSYLIIQFVLKTIGINWDGTKTNIGLYSQENILA"
     gene            complement(10012..11382)
                     /locus_tag="PKK65_02870"
     CDS             complement(10012..11382)
                     /locus_tag="PKK65_02870"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNU81653.1"
                     /translation="MFKKIISLFLLAIFILTSGFGCKTVDKQTQTAMQPITLTYWRVF
                     DDSDAFQEIIDKYKAIHPNITINYRKLRYEEYELELLNALAEDRGPDILSLHNTWLRK
                     YQNKLTPMPASITMAYPIEQGTVKKEVVPVLKTVKSLSLNDLKNTFIDVVYDDVVLDD
                     NQIYGLPLSVDTLVLYYNRDLLNNAGITQAPKYWNKEFQQNVKKLTKQDPVQGITQAG
                     IGLGGSTNIERYSDILSVLMMQNGATMMDGKQVLFNTIPPTAQDTKYNPGLEALRFYI
                     DFSNPGKEVYSWNSDLPNSLNLFISGKLAMLFGYSYHAATIKAQAPKLNFSISALPQI
                     EGSPVEVNFANYWVESVSKKSKYPNEAWDFIQFATKEEQVKSYLTKTKKPTALRSLIA
                     SQREDNDLSVFASELLTAKSWYRGKDANAAEVAIGEMIDNARQNPNKILDAINTGAAK
                     VQQTVN"
     gene            complement(11545..>12615)
                     /locus_tag="PKK65_02875"
     CDS             complement(11545..>12615)
                     /locus_tag="PKK65_02875"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNU81654.1"
                     /translation="AQIEKDKSELNKNLANLDAKIDADQIELQKYNTAKEEEKNRLFA
                     YQSENQNLQTEINQLNGRLNNLKIELARQETRLEDLENNIADDGLELDSIKKYKPTDE
                     DNLADARLRQRIQELKSQLEMIGGIDPETEKEFQETDERYRFLDKQTQDLNTAIESLE
                     KVIAELDINIKEKFEQEFKIISEKFSEYFKILFNGGTAKIFKLETTEEDKEITDKLDN
                     NNTDERSRRLKNLRKYNAIGLAGIEIQATPPGKKIQTISMLSGGERALTAIALICAII
                     SANPSPFVVLDEVDAALDEANSERLAQILDDLSSKTQFVIITHNRALMKRASIIYGVT
                     MKADGVSQLLSIRLDDINAFRN"
     assembly_gap    12616..12715
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12716..14601)
                     /locus_tag="PKK65_02880"
     CDS             complement(<12716..14601)
                     /locus_tag="PKK65_02880"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNU81655.1"
                     /translation="MYLEKLEIQGFKSFANKNKLVFSNKIDANGHRGLTAIVGPNGSG
                     KSNIADAIRWVLGEQSLKTLRGKKSEDVIFSGSDQKSQLSMAEVSLYLNNEDHQIVPV
                     KNNEEETDTSGHSLSPLNYDEIVITRRLFRNGDSEYLLNNNRVRLADIQILLAKANVG
                     QKTYSVIGQGMVENFLNTSAAERKDFFDEATGVKQFQIKRDAALNKLESSYDNLLQVE
                     MLLNEIKPRLKMLTRQVERLKKRGQLETELNIRQHRYYFQLWQDIDSKLKQANSGILL
                     LEKEKQEKENNLEKIKAELNTLKNKDDSSKISQLQERLKIYQRERSDIVQSIARLDAE
                     LELQLESQGQSDIAWLNGKQRELKEERRRLSQEISNLENEDISAKETKLRAKIQKLNE
                     EINRRENFRREYERQQEEKSQLLKNLARLDAVLEANLEAQGQFDVAWLNNKQEELKRA
                     LDSLKKEIESINIEDSKNAVTELENKKADLDNLIARDNQALQALNNFLKNSSQPAAGR
                     AEINESIDEFLKKLDAIKDEQNLERIKILIEEAKQTFQKQIRSLIDGENAAKLEQVKA
                     LQENIIKNSEARQILIAEINELQLELSTKQERRRLLENSREASIREIKDIEDKIAKSQ
                     IKFN"
     gene            14769..15332
                     /locus_tag="PKK65_02885"
     CDS             14769..15332
                     /locus_tag="PKK65_02885"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HNU81656.1"
                     /translation="MIISLSGAGGSGKSTIAMKLADKLGWPRYYIGGLRREMAARRGL
                     TLAEYNKLGESDPATDMEVDDYQKKLGETEDNFIIEGRTSWYFIPHSLKIYLDVDADE
                     GARRVFAHLQQKNKRNEDNGLDSVEAVKRSIAQRLESDNLRYRKYYGIKGYNRENYDF
                     YLDTTNLSVDEVFNQVYNFVRSRLDKH"
     gene            15420..15779
                     /locus_tag="PKK65_02890"
     CDS             15420..15779
                     /locus_tag="PKK65_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNU81657.1"
                     /translation="MSEKKTDSVKEKKPTELELKPGMTVRIYQKIKELNSKGEEKERI
                     QYFEGMIIARKHNKEKGGTITVRKVSEGVGVEKIFPLNLPTITKIEIKKIAEVRRAKL
                     YFVKRGYKKRLKEKVVR"
     gene            15829..15915
                     /locus_tag="PKK65_02895"
     tRNA            15829..15915
                     /locus_tag="PKK65_02895"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15863..15865,aa:Leu,seq:gag)
BASE COUNT         4342 a         2869 c         3230 g         5453 t
ORIGIN      
        1 attattttag ccgataagtt ctgtgacatt tttgacggct tttctatcgg ttccaatgat
       61 ttaactcaat tgttattagg gattgatcgt gattccggcg gcagtttaaa aattaaaggc
      121 gtttccaatg aaaagaatga ggcggttaag acgatgatta agaatttaat tgccgtggct
      181 aagaagcgca agcgcaaagt tggtatttgc ggtcagggtc caagcgattt tcctgatttt
      241 gccgaatttt taatggctgc cggcattgat agcatttctt tgattccgga tacggtaata
      301 ccaacaacca tgaatttagc tagtaaggaa aaatctttgc gtcgccgcgg ttttctctcc
      361 tttctttttt aattaaagtg ctttaaacaa aacacccgct acggtaattc cgcagtgggt
      421 gtttttgtaa tcttgaaaat tatcaatcaa tgaattccac cacttctatt ttggcgactt
      481 tgacgccgaa ttggcgggcg tcagtttttt ctttcatcca gatatcaata cgatcgctat
      541 agcgcttgtt catgcggtcg cgcaccacaa agatgcggtc gccatataat tcggggattc
      601 tgactttagt gccgaattga aggaagttag cggcaatcgt gtcctcttcg ccatgtttac
      661 agacattgaa accattggcg gtaatacagg gggtgttgtc agtctgggcg gggtcagagt
      721 tataggcagt gatagtatgg gtgctggtcc taatcacttt aattgccgga gtgtttgttt
      781 ctaatttatc agtggcaact tcaacatttt ccgtatcaac ggtaatattt gcatttttaa
      841 ttaaatcagc tactattggg tcggcttcgc tcttgctggt agtatccatt tttatatctc
      901 cttgagctaa ggtcacggct tcagccgcta agacgggtgt aaagaagaga acaaattgga
      961 aaatacaagc aataatcaga gtgagagcgg ttctttggcg tctactaaga ggcactaagt
     1021 cctcgctgat tttgaatttt ttcatattag cgtaaggtta ttttctcaaa caaaaaatcc
     1081 ctacagtatt aaatatgaca agcagggatt tttgcgactt atctttatat catagcttaa
     1141 ttgttaagtc aaggtgtaga ctggggataa gttattgaca tatatttaaa aaagtgctat
     1201 actaataagt tctaagctaa gattgtaaaa ttttagcgaa gaacataata aattgtacat
     1261 taacattaac aacaaaaaat atccatcatg aaaacacaga aattttttct atttttggcc
     1321 atttttatgg ctgtaactat tggtattaac gcccaaaccc gccagcagcg taaggctctg
     1381 gaaaaagtgg caactgaaaa agccgctctt gaaaaagagc tggccgaact tgaaatctca
     1441 attgcgactt atgtcgcgat tgacacactg cccacctact ctcgtaaaaa ggagattgac
     1501 caagctttgg caagcggcga aatgtccatt aaacaaaaag ccgcttatag cgatcaaagt
     1561 cacaagcttg gtcgaaagct ggcggctatg cgtcaacaat tgttggttga tactgcttct
     1621 tttgcttatt gttccagaag aatcaatgaa attaaatttc gtcttcagaa gctgaatgat
     1681 tatcagaccg acctgattaa caatcagctt gacgaggcag ttaatcagat accgcctcaa
     1741 gagatgagct cacgtgaagc taaacgccgt catcgcggca atattataaa gatggaaaca
     1801 aacaaagctg cttttcaaga aaaattgcag gcaatgtctg tttcaaaaaa agaggccgct
     1861 cccgttattg ctgaccctat taatggtttt tttggtaggg ttataaatcg ctcaagccat
     1921 acggtctata atttcgttat ttacgaaaaa aacagcaaag cagaattaaa atcttttgtt
     1981 cttgaaccag gaacatcggc aagtaactat tttctgccag gagaatatgc ttgtgatgtt
     2041 aaggcttatg gagttatagt agctcattat gagtttcatg ttacagcaga tactcatgat
     2101 gtttttggtg aagagctcca ttgggcggtg tggcaggagt cagaggttag aaaaccaatg
     2161 gccaaaaata taactttaaa ccattgatct tttataaaag gccagtcttt cctagactag
     2221 cctttttctt ttacattttt tatttatgga gttgctccac caccaggaat actgttatta
     2281 cccgttccta ttcccccgcc gccactaaag ggtagttggt taaagataaa ataagtgatg
     2341 gcgtaggcca ttaagacaat tactaagccg ataattgccc gcttaatcat gtcttgcgct
     2401 tttttaaccg cctcttcatt accggcggca gtcatccagc tgtagccggc aaagacaata
     2461 ataattaaga agattatgcc cagcaggctt aaagcgactt gaatgattgc gacgataatt
     2521 tgaactaaag taaaatcatt taatccggtt tctcctaaca ttttttgagt tggttcatca
     2581 atagtttcga ccaggctctt agcggtggtt gttcgcgctg atacaaccga cattggcaga
     2641 ataatagcta agactgcgat tattaatctt attttaatat ttttcatatt tataatctct
     2701 caataatatt cactaaaata aaattccaaa ttgcccagga actaaggaca ataatcaggc
     2761 ctatgagggc gtgttgaatg gtgttttgcg cttttttaac cgcctcttca ttaccggcgg
     2821 cagtcatcca gttataacca gccaagatca caagggctat aaaaataacg cccagcaagc
     2881 caagggcacc attaataatt agaccaataa tagtgagaag cgagttttca tcggctttag
     2941 tatagggacc attttctccg gaagcgatat tttccaggat tgttaaagga ttacctgatg
     3001 agctggtatt aacggaacca gtgctggtgc tttgggcaaa tacgaaatag ggcagaatca
     3061 taatggcggt gagcagtaat agggtgaaga ttggttttaa agattttaac ttcatacagg
     3121 ctttaaataa agaatttgat gatgaaataa gttaaggcgt aagctcctaa aataatcacc
     3181 agaccgacaa tattttgggt aataattgat gtagcttttt tagtctgctc ttcgttgccg
     3241 ctggcagtca aatagagata gccgccgtaa ataatgaaaa taataaaaat aacaccaagt
     3301 aaggaaaata atccagttat tattctgccg acaactgttt ctaaaacaac attattgtta
     3361 taaccagcgc cgccagcgcc ggaaacaatt cctaaagcgc tgctgctgct cgtgtcaaaa
     3421 gcagttttta aattagcgtt agtttgttcc tgggccgcga ccgataatgc cccagtgaaa
     3481 ataaacaaag ttagagcaat tataatgata ttgattttgt gtctggcaga catgttttta
     3541 ttgaattaag gagccgcttg tcgcctttaa aataaaataa ctgatagcat aagcggcgag
     3601 gacaataata ataccgatga ttgattcggt aagcatttct ttagccttgc tgaccttttc
     3661 ttcgctgccg ctagcagtca tccaaataat gccggcataa atagcaaggc ctaaaaacac
     3721 aacgcccact aaggataaaa taacagtaat gatttgcgca atataatcgt ttattgttgt
     3781 gtcagtagtt ttgtagccag ccttattgcc agtattactt aagccgctat tttgggaaaa
     3841 atcataattt ccgttttggg ctagagctaa attaaagacg cctaaagaca gaataaagat
     3901 aaaaattaag gaaattaagc gttttgcccg catattaagt gagcttatcg ccgacaaagg
     3961 cggtaatagc ataagcagcc ataacaatta ctaaaccaat cacagcatta atcattaaat
     4021 ctttagcttt gttgactttt tcttgattac cggaagctgt catccaggat agaccggcaa
     4081 aaatcatcag gattagaaaa attacgccga caaaagacaa gactagtccg ataatagaac
     4141 cgagacgggt actgagaaaa ttgttatctg gattagccgc ttgggttttg taagcgtcaa
     4201 ctacattagc gctttggctt aatccatagt catcagctga aaccggtaag acactaaaaa
     4261 aaccggcaat tataaggctg gttatcaaga atgaaaatac gctttttgtt atttgagcgc
     4321 gtgacataga tttatttatt ttttgtttca ctgctctaaa cgccaatgat ttgtcgttta
     4381 gagcggtggc ccccttatta aagagggcct tttccaaaat cgattctaga aattactaac
     4441 tagaaattac taagtagtag cagaaattaa gcgtcctaaa atgaagctgg cgatacccca
     4501 agcggctaag acgataacca aaccaataac accggcgccc attaattttt gagcttcact
     4561 aaccttgtct tcgttgccgc cagcggtcat ccatttaaag ccacccacta agatgatgac
     4621 gacagcgatg atgcctaaga aaagcatagc gacgttaatc aatctagcga tggtggttct
     4681 cggatcggta ttacccaaac cgttatcacc aaaaccttct tcgatttcgt ttatgccgac
     4741 atcaatgttt tgcgccattg taactatcgg tagagccaat aatgacaaca tcgcaaacac
     4801 cactaaattc tttaagattt ttttactcat aattcaccac ccttctagtt gttgatattt
     4861 ttagatgttt ttattttatc ataatttata ccgaaagaca aacaattttt cttatgataa
     4921 aatataaaga tgaataataa aattaataaa ttgtcaatag ttattttagg gttcttggtt
     4981 tttgtagccg ttgctctttt ttttctatca aactcttatt cttattcaga tgtagattta
     5041 ggttggcatc tgcgtatcgg ccaggatatt ctaaaaagcg gtcaagttcc cgtttttaat
     5101 gactataatt atactctatc cgggcagaag tgggttgatc atgaatggct tttaaatgct
     5161 ggcatggcgc tattgtttaa tcacggtaaa tttatcagcc tacacttagt tttcctttta
     5221 gtcttgttgg tgacggcttt ttttgtttgg cggcgaaata ttaatctctt aggcaacaaa
     5281 atagtgttta aactcggcag cgctatctgg ttatttttag gtttattggc cagcaggcct
     5341 catttaggca tccgggttca agagtttggc ttgttgggta tggcaatttt gttttggatt
     5401 ttttctgggc catcccgctc tcggcactat ttttggtatt taccgcccat attttggttg
     5461 tgggccaata ttcatggcag ctttctttta gggctagcct tgttgggcgc ttattgtata
     5521 tatgcttttg cctctccact ttttaaacat gtaaatcatt ggcagttatt tagagttgat
     5581 agttttgatc ttaagttcaa aaagcaatta ttggttattt ttactttgtc tgttttagct
     5641 actttaatta atccttacgg tgttgatttg tatgcttttt tgggcactta tacgcataca
     5701 acctatatgg tttatatccg cgagtggcag agccagtttg tttttccgct tttttatgtc
     5761 cagatatttt atctagcctt ggccgccgtc gccttgatca tgttaataaa ggataggcgc
     5821 agaaacttgt ttatattttc ttggtgggag gcttttttat tcgtattttt ctttattttg
     5881 gcaattaagt cgcgacgcca ttttcctcta tttgttttag catctttacc ttttttaagt
     5941 gtggcgtttc aagagtttag cgctcagatt attggtttag ccaagcgctg ccgttcttta
     6001 atctggaggg cgttggctat ttttctaatg tttattttag ttatttatca agccgttaat
     6061 attccctggt ttcaaaacag tatccaagat ttttgcggac gaaaatatcc ttgcgcggct
     6121 gtcgagtatt tgcgggctag accggattta ctaaataaac gtttattcaa cagctatgat
     6181 tggggaggat atctattatg ggcttatcct gaaagacaga tatttattga tggtcggatg
     6241 ccgcaggcag agtatcggca tcacactatt ttagaagaat atttaagatt taggagcggt
     6301 agtgaagata tcgggaatct actgaatact tatcagattg atttggtctt attgaaaaaa
     6361 agtgtcagcc ctttagctct gcgctcttgg gaaaaattat tttttcagat ttcagaggaa
     6421 gaattaacag cgcctgatta tttgcgctta tatttagatt cttcccttga ttggcaggtg
     6481 gtttttgaag ataatataag cattatctat gaacgccaaa aaaattaata tttggaaaat
     6541 tttattttgg tttgctctgg cggttttggc agtatttttt cattctgatc attttttaaa
     6601 cggcgatgaa ggggtcactt taaatggcgc ttggaatttg tataatcatc gtcatcttta
     6661 ttttgatttt ttttcttttt tgccgccttt aagtttttat cttatatatt ggatatggct
     6721 gattttaggt gtaagttttt ggtcagctaa agctttttcg attattttac tattagctgg
     6781 cgcttttggt ctttttaaaa tctccgaaca tttacgttcc cacccgatta attattttgt
     6841 cccttttctt tttattttaa tttcttcttg gtggtggatc attaatcata atttttatta
     6901 tctagttttt agtatttggt cctgttattt cttgttattg tttttagaaa ataatacccg
     6961 tcgtcattgt ctggcggccg ctttattggc atcagccagt atcgccactt tgcaacaaaa
     7021 gggtttgttg tttgccctgg cttctttttt gattatatta ttttttaata aaagacaatt
     7081 tgggcgtcgt cgctttttag cggccgcgct ttatgtgtta ggcgtagcgg ctcccttggt
     7141 tttgttatta tttttttggc ccttatctgt attatggcag aatttggtaa tttttcccct
     7201 attccattac attgaggcca atcgcctccc atattattta ttatttttag cttctatatc
     7261 ctggctctta ttcgcttggc ccttccgttc ttatgattat cggcgctggg cgctgttgat
     7321 tttaagtggt gttttaatta tttcctgcta ccctttgcct gattattatc atttaagtat
     7381 ggctttagtg ttgttattgc cgatgttgcc tgatttgttg gataagttaa ataatcccgc
     7441 tggaagatgc cgtcgagttt tagcaatttc tggtttaacc tggctttggc tctggccggt
     7501 aagccatttt ttagccttct tctttaactt ttctttcact aaagattatc cttttttgga
     7561 ctatatccgc cgagaatgtc cagggcaata tctgcaagtt ggaccctttt tacctaatgt
     7621 ctatttggaa accggcaagc ttagcgctac tagttttgat attttaatta ccggacatca
     7681 aactgccgag caatttgccc aggcggccgc gcagttggaa ataaatcaac ctgtttgtgc
     7741 ggtaacggcc tatccggcgt cgctttcacg ttttcgccac caacttgata atccggtaga
     7801 taattttatt agagcaaatt atcagccggt atttaccgag gggagtatta ccgtatggaa
     7861 atacaggaat caataaattg gcttttattt gtttccgagt ttaaatttag taatttaaaa
     7921 atccctccga tatcggaggg atttctttag ggaatgtatt ttttaaattc ttgttcttct
     7981 gccaactgtt tttgcttggt cggcaccatt cggtattgtt taataattgg ccagagccac
     8041 cataacataa taattaccca gattgcgagc caaaaacgag cggaaagaaa agccgcttgt
     8101 tcatagttaa agaagaagaa ggcggtgccg atgattaaat tgccgatact aaaattataa
     8161 gcttttttcc aaaatatttt atataaactt ttacgatttt tcttgatgcc ggtaaaaata
     8221 gaggctaata gtagtaggat taaaaatccc aagaaaattt gtacgaaaat aggcaatagg
     8281 ggttccggcc gtaaattgaa ccaaaaatgc caggtaagca gattttgcat agtgtttata
     8341 ctttataaaa tattaatccg tagtgataag ggccagcttc aaattcagtt ttaaggttaa
     8401 gacctaattt aggagccgct tctttcagcg attctttgct caccctttgt tctgatcgtg
     8461 gaccgaaagg gctgtcgcta tttttccatt ctacaactaa gagctggcta ccatttttta
     8521 acatccgtac agcttctctt agtatttcca ctcttttatt tgattgatgg agaatattta
     8581 ttaatagagc ttgatctaaa ctggcgcttt ctattttagt gcccttaaat atttctaagt
     8641 tactccagac ggtttctatt tgttgtaaat tttcttgcaa ggcgcgtttt tttatatctt
     8701 caagggtgtt ttttagtata tcaacagcaa aaacgcggcc acgggctccg accattctgg
     8761 caaccgggaa aacgaaatag ccgaaatttc cgcaacctaa ttccgcgacc ctttgacctt
     8821 cggttattgc tattttattc aaaataaaat ttatgtcaaa tagcatggtt ttattgggac
     8881 tgctcataag tgttttcgac cagaatttgg cgctgaataa cgctttcccg gccatatttt
     8941 tttttagcgc tgatggtaag gttgtttaag ccttgtttaa gttttatttc ttggctgaaa
     9001 tgaccgtctt ggtcgctcat aataccggtg ccattaatct ttatttctgt ttccggctcg
     9061 ctttttccct gaatgttaat aattggattc tgtaatacta gatttttatc tggatattcc
     9121 agtattagtt cgggcggact cactaaacgg aaaaaatata tgctgaggta taagatacag
     9181 attaagaata agagtataag taaagcattc ctgattattt taggaaatat taaaaattta
     9241 tgacgtctta atattttttg agaaaaagga ttatggctaa ccgcttcttc cgcaaagggg
     9301 ctaagtttta atatgtcttg ataatttagg ttaaggtagc gacaatattc tttaagaaat
     9361 tggcgtccat ataagccgga gggtaagcgg tcatattctt cattttctag agataataaa
     9421 tactcttctc gaatgtgaag tttttcggag gcgtttttaa tgtcaatatt ttggcgcaaa
     9481 cgagccgctc gcagtttttc tccagatctt tcttcgctcg ctaatttctt aaagttaaat
     9541 tctaccattt ttaatttagg ctaaaatatt ttcctgggaa tataaaccaa tatttgtttt
     9601 ggtgccatcc caattaatgc cgatagtttt aagaacaaac tggataataa gataactgct
     9661 aaatacgatt attaagccaa ctacggcggc gacgataatc gctttggctt tgctgatttt
     9721 atcactgcta ccggatgata tgaggaacat aaatccgcca tagacaaaca ttaataaagc
     9781 taaagagccg acaattccta aaatccaacg ggaagcgcgg acggcaatca tcacaatatc
     9841 gtctaaggtg taatcgccgg tactatattt agtgtcacct tgattgacgc cttcagtagt
     9901 tgtgctagtg gtgtctacaa tagagattga ctgggctaag gtgggtaagg ctggtgataa
     9961 aaagaaggaa ataataaagg ctaagccaat aagtgctttt ttaatcatat tttaattgac
    10021 cgtttgctgc actttggcag cgccggtgtt aatagcgtct aaaattttat taggattttg
    10081 acgggcatta tcaatcattt ctcctattgc cacttcagcc gcatttgcgt ctttgccgcg
    10141 gtaccagctt ttagccgtga ggagctcgga ggcaaacaca cttaagtcgt tgtcttcacg
    10201 ttgagaagca attaaagagc gtaaagcggt aggttttttg gttttagtta aataggattt
    10261 aacctgttcc tctttggttg cgaattgaat aaaatcccaa gcctcattcg gatacttact
    10321 cttttttgat actgattcta cccagtagtt ggcgaagttg acttctaccg gactgccttc
    10381 tatttgtggc agggcgctaa tagaaaaatt aagttttggc gcttgtgctt taatggtggc
    10441 ggcatgatac gaatatccaa acagcatggc cagttttccg ctaataaata aattaagaga
    10501 attaggtagg tcgctgttcc aagagtatac ttctttcccc gggttgctga agtctatata
    10561 aaagcgtaga gcttctagac cgggattata tttagtatct tgcgccgtcg gcgggatggt
    10621 gttgaatagg acttgtttgc catccatcat ggtagcgcca ttttgcatca ttaatacgga
    10681 cagaatatcg ctatagcgtt caatatttgt gctgccacct aaaccgattc ccgcttgggt
    10741 gatgccttga accgggtctt gtttggttag ttttttgaca ttttgttgaa attccttgtt
    10801 ccaatatttt ggagcctggg taatgccggc gttattaagc aggtcgcgat tataataaag
    10861 gactagagtg tcgactgata ggggaagacc ataaatttga ttatcgtcaa gaacaacgtc
    10921 gtcgtatacc acgtcaataa aagtattttt taagtcattt aaactgaggc ttttgactgt
    10981 ttttaaaacc gggacgactt ctttcttaac ggttccttgc tctatcggat aggccatggt
    11041 aatactggcg ggcattggcg ttaatttatt ttgatatttt ctcaaccaag tattatgtag
    11101 ggataagatg tctggtccac gatcttcggc tagggcgttt aacagttcta attcatattc
    11161 ttcgtagcgt agtttacggt agttaatggt aatattggga tgaatagctt tatatttatc
    11221 aattatttct tgaaaagcgt ccgagtcatc aaaaacacgc caataagtta aggtgattgg
    11281 ctgcattgcg gtttgagttt gtttgtctac ggttttacaa ccgaatccgg aagtaagaat
    11341 aaaaattgcc aataaaaaca ggctaataat tttcttaaac atagaacgtt atgatttaat
    11401 tatcccctga ccgaccggcg ggcgattatt attattataa cagaaaatca gggagaaagt
    11461 aaattaaggg ttatagtttt tatataaata aaactgcttc ttggcgagca gttttatggt
    11521 ttttctaaaa ttaaaatcta atttttaatt acggaacgca ttgatatcat ccagcctaat
    11581 acttaatagt tgactaactc cgtccgcctt catggttacg ccgtagataa tactggctct
    11641 tttcattaaa gctcggttgt gagtaatgat cacaaattga gttttagatg aaagatcatc
    11701 aagaatttgg gctaatcttt cagagttggc ttcatctaaa gcagcatcga cttcgtcaag
    11761 aacgacaaaa ggagaaggat tagcgctgat aatagcgcaa attagagcaa tagcggtaag
    11821 agcgcgctct cctcccgaga gcatagaaat agtttgaatt tttttaccgg gaggcgttgc
    11881 ctgaatttca atgccggcca aaccgatagc gttatattta cgtaggtttt taagccggcg
    11941 tgagcgctca tcggtgttat tgttgtcaag tttatccgtt atttctttgt cttcctccgt
    12001 tgtctctaac ttgaagattt tagcggtgcc tccattaaat aatattttga aatattcact
    12061 aaatttttct gaaattattt taaattcctg ctcaaatttt tccttgatat taatatctag
    12121 ttcagcgatg actttttcta gggattcaat agctgtattt aaatcttgag tttgcttgtc
    12181 taagaaacgg tagcgttcgt ccgtttcttg aaattctttt tctgtttctg ggtctatacc
    12241 gccaatcatt tctaattggc tctttaattc ttggatgcgc tggcgtaagc gcgcatcagc
    12301 taaattatct tcgtcggtgg gtttgtattt tttgatggaa tctaattcta ggccgtcatc
    12361 ggcaatattg ttttctaaat cttcaagcct ggtttcttgg cgagctaatt ctatctttaa
    12421 attattgagg cgtccgttga gctggttgat ttctgtttgt agattttggt tttctgactg
    12481 ataggcaaat agtctatttt tctcttcttc cttggcggta ttgtattttt gtaattcgat
    12541 ttggtccgcg tcaattttag cgtcaagatt agccagattt ttgtttagtt cgcttttatc
    12601 tttttcaatt tgggcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnctgtt
    12721 aaattttatt tggctcttgg caattttatc ttcaatgtct ttgatttctc gaatagaggc
    12781 ttcccggcta ttttctaata aacgccgtct ttcttgtttg gtcgagagtt caagttgtag
    12841 ttcgttaatt tcggcgatta aaatttgtct agcttcgctg ttttttataa tattttcttg
    12901 aagagctttg acctgttcta gtttggcggc attttctccg tcaatcagag aacgaatttg
    12961 tttttgaaaa gtctgttttg cttcttcaat caggattttt attctctcta agttttgttc
    13021 atctttgata gcgtctaatt tttttaaaaa ttcatcaata ctttcattta tttccgcgcg
    13081 tccggcagct ggttggctgc tattttttag aaaattatta agcgcttgca aagcttgatt
    13141 gtcacgagca attaaattat ccaaatcggc ttttttattt tctaattctg taacggcatt
    13201 tttactgtct tcaatattaa tagattcaat ttcttttttg aggctatcaa gcgctcgttt
    13261 gagctcttct tgcttattat taagccaagc gacgtcaaat tggccctgcg cttctaaatt
    13321 tgcttccaag acggcatcta agcgcgctaa gttttttaat agttggcttt tttcttcttg
    13381 ttggcgttca tattctcgtc taaaattttc acggcggttt atttcttcgt ttaatttttg
    13441 gattttagcg cgtaattttg tttctttagc gctaatatct tcgttttcta aattgcttat
    13501 ctcttggctt aaacgtcgtc gctcttcttt taattctctt tgtttaccat taagccaggc
    13561 aatatcggat tgtccttggg attcaagttg aagttctaat tcggcatcaa gacgggcgat
    13621 gctttgaaca atatcgctgc gttcgcgctg atatattttt aacctttctt gaagttgact
    13681 tatcttggaa ctatcatctt tgtttttgag ggtatttaac tccgccttta ttttttctaa
    13741 gttattttct ttctcctgtt tttctttttc aagtagtaat atgccggagt tggcttgctt
    13801 taatttggaa tcaatatctt gccacaactg gaagtagtag cggtgttgtc tgatatttaa
    13861 ttcagtttct agttgcccgc gtttttttaa gcgctctact tgacgggtga gcattttaag
    13921 gcgcggtttg atttcgttaa gtagcatttc tacttgcaat agattgtcgt agctgctttc
    13981 caatttgttt aaggcggcat cgcgctttat ttggaattgt ttgacgccag ttgcttcgtc
    14041 aaaaaaatct ttacgttcgg ccgcgctggt attgagaaaa ttttctacca ttccctggcc
    14101 gataacgctg taggtcttct gacccacatt ggctttagct aagagtatct ggatatcggc
    14161 taagcggaca cggttgttat ttaataaata ttcactgtcg ccgttgcgga aaagacgacg
    14221 tgtaattaca atttcatcat aattaagcgg cgataaagaa tgtccgctgg tgtcggtttc
    14281 ttcttcgttg tttttaacgg gaacgatttg atggtcttcg ttattgagat ataaagatac
    14341 ttcggccatg ctgagttggc ttttttgatc agagccggaa aaaataacgt cttcactttt
    14401 tttgccgcgt agagttttaa gactttgttc ccctagtacc caacggatag catcagcgat
    14461 attggatttt ccggagccgt ttggaccgac gatggcggtc aggccgcgat ggccattagc
    14521 gtctatctta ttagaaaata ccaatttatt tttattggcg aatgatttaa atccttgaat
    14581 ttccagtttt tctaaataca taaaaaagcg cgcggacgcg gaattaatta agaaaaagcc
    14641 catttcaggc ttgctccttc tttcaaatat gttattatta taatatattt tgttgggaaa
    14701 agtaaaatga aaatttatta aggcagaagc gtataattaa ttccatcagt ttcgcgcttt
    14761 ttgtttttat gattatttct ctaagcggcg ccggtggttc cggcaagtca actattgcta
    14821 tgaagctggc cgacaaatta ggctggccgc gttattatat tggcggtttg cgtcgtgaga
    14881 tggccgctcg gcgtggtttg actttagcag agtataataa attaggagaa agcgatccgg
    14941 ccacagatat ggaggtcgat gattatcaga aaaaattggg tgaaactgaa gataatttta
    15001 taattgaagg ccgcacctcc tggtatttta ttcctcattc cttaaaaatt tatttagatg
    15061 tggatgctga tgagggtgcc cgtcgagttt ttgcccattt acagcaaaaa aacaaaagaa
    15121 atgaagataa tggtctagat agcgttgaag cggttaagag gagtatcgcc cagcgtttgg
    15181 agagcgataa tttgcgttat cgtaaatatt atggcataaa aggctataat cgggaaaatt
    15241 atgattttta tttagacact actaatttaa gtgtagacga agtctttaat caagtctata
    15301 attttgttcg ctcccgcctt gacaagcatt agttttgatt ataatattag acaagactta
    15361 agtaatttag actgagcgag gtgtcaagca tctcgtttct ggtttttacc aaaatatata
    15421 tgtccgagaa aaaaactgac tccgtgaagg agaaaaaacc cactgagctg gaattgaagc
    15481 cggggatgac ggttagaatt tatcaaaaaa ttaaagagct taactctaaa ggcgaagaaa
    15541 aagagcgcat tcaatatttt gagggtatga ttatcgcccg taagcacaat aaagaaaaag
    15601 gcggaactat taccgtccgc aaagtttctg aaggtgtggg tgtggaaaaa atcttccctt
    15661 taaatttgcc gaccattact aagattgaaa tcaagaagat cgcggaagtc cgccgcgcca
    15721 agctttattt tgtgaagcgg ggttacaaaa aacgcttaaa agaaaaggtg gtccgctaac
    15781 gcattttaac acgaaattat taaaggggct tttgcccctt tatctgaggg gcggatggtg
    15841 aaattggtag acacgcaagc ttgaggggct tgtggccgca aggccgtgga ggttcaaatc
    15901 ctctttcgcc caccatcttt attcgggcgc atagctcagc tggctagagc acttcgttta
    15961 caccgaaggg gtccagggtt cgagtccttg tgcg
//
LOCUS       DAOQSA010000020        15136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_2993_length_15136_cov_8.809031, whole genome shotgun sequence.
ACCESSION   DAOQSA010000020 DAOQSA010000000
VERSION     DAOQSA010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 15136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15136
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2993_length_15136_cov_8.809031"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1643)
                     /locus_tag="PKK65_02900"
     CDS             complement(<1..1643)
                     /locus_tag="PKK65_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HNU81658.1"
                     /translation="MEKMRSISARLFPEKLPSPEELEKRYPPRLLPAGALVTRVAPSP
                     TGFVHIGGLYASLISERLAHQSQGIFILRIEDTDKKREVVGAANLFAAALAKYNIKVD
                     EGPDENGLEKGNYGPYKQSARAAIYQSYIKNLLNQGLAYPCFATPEELEELRGQQEAR
                     GERFGYYGHFAIWRERSPEEVSAALDKGLKPVIRLKSPGNFHNKIIVNDLLIGRREMP
                     ENDQDIVIMKSDGLPTYHFAHAVDDHLMGTTHVIRGHEWFPSLPLHLQLFQVLGWKAP
                     AYAHLAPIQKLDEGKKRKLSKRQDQEANLQYYYAAGYPEEAVIEYLLNLANSDFEDWR
                     KNNPDTPNSEFKITWKKLANSNGPLFDAAKLEDISKNIISRLDAKTVYQRTLDWAKIY
                     DTELADLLKAQADYSQKILNIERAGGLQPRKDIGRWSEVKEEISYFFDHNFNLNPEEL
                     NSIKEELGNIDTDAFINEFLSTYDINDDPETWFEKLKNIGEKYGFAKSVKDYKEAPEK
                     YKGQVGSLAKLLRFRLTGRLRTPNLCLIMQVMGEEMIKK"
     gene            1844..2431
                     /locus_tag="PKK65_02905"
     CDS             1844..2431
                     /locus_tag="PKK65_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81659.1"
                     /translation="MSTDEQLLAAQNTKNQPDQENSSSDRAGALREQQREGGGQEETG
                     QSLRSQVMAARKDQASEDAKKNEERKSISFRHSQILKQNIFNLVTSFGLSYFYIVYHW
                     FQSKLGNKRKYVKLGSEWMDSPALTEKQRDEIGSNFAPWENCCFFSVSIGCLVAIFLV
                     ILPLALIGYYLSHPAEAAWEGLKSVWEIIKSIFTS"
     gene            2436..5168
                     /locus_tag="PKK65_02910"
     CDS             2436..5168
                     /locus_tag="PKK65_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81660.1"
                     /translation="MRLPVRFYLKRRFWSVFLLSIVFFSVALPVYANWSDTLGSIVGW
                     LIGLFISALGLVLILVIKGLLLIASYQNFIGSPAVFEGWAIVRDLSNMFFVVILLVIA
                     FSTILHLENYSYKKWLPKLILMAVLINFSKTICGLLIDVAQIVMLTFVNAFKDIAGGN
                     FIDMLGIRDIVTMAKNTDDLGFWAIVGAYVLGLIYMIVAVVVIATMMMMLVMRLVMIW
                     IYVVLSPLAYLLAAFPGGAQYSSKWWKDFTQNLIVGPVLAFFIWLSFAALQNGNELDI
                     VRTAKDNSVNEEIAAVGAPTNSKEKMGSPVAITEASTPDALIKFVIGIGMLVGGLKIS
                     QEIGGAAGSIAGKGMNKLSKMGAATTGFIGKGALGISKDLGMGLAKKAKVKEGLGYIS
                     SRTGAVGRALAVTGIRGLATKGLVSLNSQEAQIKEKAKKKLSNIDDTRVMARYANESA
                     FGGMGIAMRKKARTKMPDAIESDQEMNDVLASMSLDDLKELSDARWTALGTRRAHLGG
                     RSRNFINKDSDARGAFNLGMQSVGVPESEFIVGTDRNGEPLDNPDRYGSYMNPRNLPL
                     SPDEIDTLFSRGRHRFDYYKDPRPIEADTTEKVSPEKQNQERGKGDLAINGFAAGRST
                     LAVDFDQLNLEDIEKGSAGDKDWRNVRGVNTSDPAMINKIASRLTSLIDGEISSLQAK
                     GNLSKGEEKRLSSLNEAKNRLSNPENLSNLALVNSSASRFKMSDVKETIIHEEIHGLG
                     YEDEEEVRRATSTIMATRNYDARKDKASIDAILGKQPLATRPVDQIIAAEKESGPVFD
                     DSPQTVDFDLSPIEQKLANFAKQLESAGSKISNLKISGVSGSASQAPNFIYLLKALKK
                     SLTNKDDSSSRQIAALASSLGVQKAETPLEFRIIANNLPADLKPKNKVDQSQLN"
     gene            5176..6111
                     /locus_tag="PKK65_02915"
     CDS             5176..6111
                     /locus_tag="PKK65_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81661.1"
                     /translation="MNYIIESLNKFYNLPMDIRQKIGGVDSMLVVQDIEKKYGVDISF
                     ALILIAIGELNANDLSAYLQKKYFLSADDAYDIANEVTRKIFVQLFYDDNDKTNQSLL
                     IAELNEGLFNLLKDQARASIFNNNVFKILVNDASFLERLGKSLLTNELIIGNAQILSS
                     GRKVKPTVANWIKDFLTINGTDNFNDIDLIRYITDADNAKNLSKEDKSLLFKVLKTYA
                     NIAFFLENSEKKPISSLEFIPTGTTNNSDNDFLSDQADDKAILKDGADKDTVQSPANV
                     SDLDYLQKILPNYNYNSLEYKAISQEIERLRKTKK"
     gene            6200..6367
                     /locus_tag="PKK65_02920"
     CDS             6200..6367
                     /locus_tag="PKK65_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HNU81662.1"
                     /translation="MSLALSFGTFDNFHPGHAVSLQLASFWGDELLIVIAREVKGGTG
                     KIRRTGAFLFC"
     gene            6661..7206
                     /locus_tag="PKK65_02925"
     CDS             6661..7206
                     /locus_tag="PKK65_02925"
                     /inference="COORDINATES: protein motif:HMM:NF024047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNU81663.1"
                     /translation="MINFNSNKITQILAFFFLNPKRRLYLKELANVLEIDNGNLSRYL
                     KSLVEEGILKVEIEGKQKYFLLDSSYPFLKNLKKILTGSVSPEVLIKKALAPIKGLER
                     AYLFGSYASGKLKENSDLDILLIGSHDSIEVREVLASLQRRLGREINIIDYSVEEYEK
                     RLKEKDDFLDRVFHESMLTLK"
     gene            7208..7603
                     /locus_tag="PKK65_02930"
     CDS             7208..7603
                     /locus_tag="PKK65_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81664.1"
                     /translation="MFVKFIFSKQQIDKYFQAAERDLFLAANKEPEIKFQFSYNSLLK
                     LAQAVCAKQNLRVKARTGHHMVLFDKCAELLDDRKIAAVAQAMRDKRNRDLYDGGTII
                     TIKEAETYYIFIKDLVKRVKSYLNSRLIK"
     gene            7737..7904
                     /locus_tag="PKK65_02935"
     CDS             7737..7904
                     /locus_tag="PKK65_02935"
                     /inference="COORDINATES: protein motif:HMM:NF014792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HNU81665.1"
                     /translation="METFGTNKIEEDKILEAVKASFDFRPPAIINQLELKRPVFKQTA
                     AYGHFGRPEFT"
     gene            8091..8225
                     /locus_tag="PKK65_02940"
     CDS             8091..8225
                     /locus_tag="PKK65_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81666.1"
                     /translation="MARNVNVERIKNKKARQTEDQIRKKVTEFLRQQGIKGKAVFGYD
                     "
     gene            8525..>9298
                     /locus_tag="PKK65_02945"
     CDS             8525..>9298
                     /locus_tag="PKK65_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81667.1"
                     /translation="MFDYLQQFNKLPKELREKVSSPAAMAILSSLENKYGVSLAMLVI
                     QIMIKQVLIKDLVPYLIEEMSLTPDKAQSLSQELQERLFFSVATYLGLKTPPSLNPEE
                     KELQVLMKENSIILPSQELMNRCRQILLTYRKGIRTKIDVRAALEKPVAQGGLGLEVA
                     VVDRLLRSLEKSPVATSLPQASAAFTDLVNKNEASSYDLKSALARGAVKPPAELMSKF
                     KSPAAKLNLQPELEAPEKTLALEDKGTVKENLPLSVQEKS"
     assembly_gap    9299..9398
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9399..10013
                     /locus_tag="PKK65_02950"
     CDS             <9399..10013
                     /locus_tag="PKK65_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81668.1"
                     /translation="LEKSNSQVMSPQPPAAPAEKKEVEANKFAPAPKSRLWSKLFKAQ
                     EKKAEMPLKKISVSSDHLAEAVRAAAQAAPASVRPAASIETKTKMEDVRARPKVMGPL
                     EELRYLDLVNFRRLGDSPKAITDKIVMKIKLLEKDGYDRMVEGVQSWRQSPVNRIYVR
                     LAQEAVAKGITLRDAVAARQAANKETLSMEEIEAIVAMNSRLMF"
     gene            10015..10455
                     /locus_tag="PKK65_02955"
     CDS             10015..10455
                     /locus_tag="PKK65_02955"
                     /inference="COORDINATES: protein motif:HMM:NF024078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HNU81669.1"
                     /translation="MQQFTVPQFIDVESKIIGPITTRQFLIFLGAAVIIGISYKVFDF
                     SLFLTIGIIIFIIAGVFAFLKINGRPFHLFLLNFIQTMRRPSLRVWNHQLGFIDTPAP
                     MNIVKEEAPVKKKEFYQTSRLADLALVVDTKGRYRGDGDNNETV"
     gene            10460..11146
                     /locus_tag="PKK65_02960"
     CDS             10460..11146
                     /locus_tag="PKK65_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81670.1"
                     /translation="MPAKKNLASNKINVSTQQYLDIAEIKDDVVIMKDGTLRAVLLVS
                     SINFALKSEDEQNAVINSYVMFLNNLDFMLEIVIQSRELDIDNYLNYLKEKEKQQINK
                     LLKIQTAEYIEYIKELTAMGKIMNKRFYVVVPYNPLTDKHKGFFTLLGESLKPATFIK
                     LKDKSFARYKEMLNRRLDSVIGGLESMGVSVARLDTQSLIELYYKTYNPETAKNQSLV
                     DLEKLRIETN"
     gene            11156..13051
                     /locus_tag="PKK65_02965"
     CDS             11156..13051
                     /locus_tag="PKK65_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNU81671.1"
                     /translation="MINFNQNEDKQKIGLNTKISLSPSGQGQPIEANPVLTKEIIEEE
                     KAFRRGVLTVRDIISPASLQVTPKYLRLGSKFVRTIFVIGYPRYISVGWFAPIINLNL
                     TFDISMFFYPVKSAIILKQLKNKVGVLEAQIISDAEKGAARDPLRETALRDIEYLRDA
                     LTQGTEKFFQFSLYVTLYSDSLEELDRMSDQVEDIFGSRLVYSRRVYFQAEQGFNSTL
                     PLGNDELYITFNMNSSPIASSFPFISSELTSDRGILYGINRHNNSLILFDRFSLQNAN
                     SVVFATSGAGKSYAIKLEILRSLMLGSEIIVIDPEYEYKHLAEAVGGTYINISLASEN
                     KINPFDLPRAIGGEAKPKDIIRSAVITLKGLVRLMLGKLNYDEDSIVDRALLETYAKK
                     DITPDSDLSTIEAPIMSDFEQVLDGMEGGADLALRLKKYTEGTFAGLLNSVTNVEMNN
                     QLVCFSVRDLEDELRPLAIYTIVNYIWNIVRSEMRQRILVIDEAWWMMQNEDSAKFIF
                     ALVKRCRKYYLGVTTITQDVNDFLSSPYGQAIVTNSSLQILLKQSPAAIDLVQKTFLL
                     TEGEKYLLLECGVGEGIFFAGNKHVAMKVVASYAEDQLITSDPRQLLEIEEAKKEFNE
                     SLAEGQA"
     gene            13062..13682
                     /locus_tag="PKK65_02970"
     CDS             13062..13682
                     /locus_tag="PKK65_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81672.1"
                     /translation="MDKRQKLGLLIIVIGLAIIAVIIYFIFIRQAEVPATTIAPPTAS
                     TTVPLPTEPVESDITPSDKPRNYQQYDISKEPEHQTDAADVAKLSAAFAERLGSFSNQ
                     SDYSNFEDLDLFMTASMRDWAKSYVAKMRADNPYNGSYYGITTKAISAEVKSFDDDKG
                     TAEIIVGTQRREVGADGGERNFQQDLRLTFLKINDQWLVDGAYWLK"
     gene            13685..15031
                     /gene="radA"
                     /locus_tag="PKK65_02975"
     CDS             13685..15031
                     /gene="radA"
                     /locus_tag="PKK65_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNU81673.1"
                     /translation="MAIKKIYVCSHCDAQFPKWSGRCLECGSWGTLVENITDAQTEKK
                     EEAKKLGDAKVIDLRTLKGGPEIRIKSGWEEIDRVLGGGLLPGSLLLLSGEPGIGKST
                     LAAQLADALSNGHEVLYVSGEESALQVKARLERLGCDLSRLRFIGETSVEKISSAALK
                     LRPALLVIDSIQTVYSSLIPFEPGSLSQIRAVAVKFLEIAKEYNISVLLIGHITKDGQ
                     IAGPKSLEHIVDTVLSLENETGSNYCLLRASKNRFGSVNELGVLEMTGKGFREAKDPS
                     SVFLGQGSSMIAGSVVGCVLEGSRPFLVNIQALVTKTVFGYPQRQTSGFDLNRLQVLS
                     AVIAKRSKINLVTRDIILNVVGGLKINDPALDLAVCAAIISSYLNKPLPAGMATIGEL
                     GLGAELRPVFRLEQRLKEAEKLGFKSAIIPEGAEYKSGKLKLLKINNLGELVEILA"
BASE COUNT         4294 a         2692 c         3377 g         4673 t
ORIGIN      
        1 ctttttttaa tcatttcttc tcccatgact tgcataatca agcagaggtt gggggtgcgc
       61 aggcggccgg tgagccggaa gcgtaagagt ttagccaggg agccgacttg ccccttatat
      121 ttctccggcg cttccttgta atctttaacg ctcttggcaa agccatattt ttccccgata
      181 ttttttagtt tttcaaacca agtttccgga tcatcattaa tatcgtaagt gcttaaaaac
      241 tcattgataa aagcgtctgt atcaatattg ccgagttctt ctttaatact atttaattcc
      301 tctggattta aattaaaatt atggtcgaag aaatatgata tctcttcttt tacctcggac
      361 cagcggccga tatctttgcg cggttgcagt cctcccgctc gttcaatatt gagaattttt
      421 tgactataat ctgcctgggc ttttaataaa tcagccagtt ctgtgtcata tatttttgcc
      481 cagtcgagag ttctttgata tacggtttta gcgtctaagc gagagattat attcttacta
      541 atatcttcaa gtttagccgc gtcaaacaaa ggaccattgg agtttgccag ttttttccag
      601 gtaattttga attccgagtt gggggtatca gggttgtttt tacgccagtc ttcaaaatcg
      661 gaattagcaa gattaagcag atattcaatg accgcttctt ccggatagcc ggcggcataa
      721 taatattgta aattagcttc ttgatcttgg cgtttgctta attttcgctt ctttccttcg
      781 tctagttttt ggataggggc aaggtgggcg taggcgggcg ctttccagcc gaggacttga
      841 aataattgga gatgcagggg gagggaagga aaccactcgt gaccgcggat aacgtgggtg
      901 gtgcccatta gatgatcatc gacagcatgg gcaaaatgat aggtcggcag gccatcgctt
      961 ttcataatga caatatcttg gtcattttcc ggcatttccc gacggccgat tagcaggtca
     1021 ttaactatta ttttgttatg aaaattcccg ggagatttta atctaatcac cggttttaag
     1081 cctttatcta aagcggccga aacttcttcc ggactcctct cgcgccagat ggcaaaatga
     1141 ccatagtagc cgaaacgctc gccccttgct tcttgttgac cgcgcaactc ttccaattct
     1201 tccggggtag caaagcaggg ataggctaga ccttggttta gtaaattttt aatatagctt
     1261 tggtagatgg cggcgcgggc gctttgttta tatggtccat agtttccttt ttctaaacca
     1321 ttttcatccg gcccctcgtc aacctttatg ttatatttag ctaaagcggc cgcaaataaa
     1381 ttagcggcgc cgaccacctc tcttttctta tcagtatctt caatgcgtaa gataaaaata
     1441 ccctggctct ggtgggcaag acgttcagaa ataagggaag cataaagacc gccgatatga
     1501 acgaaaccgg tggggctagg cgcgactctc gttaccaaag ctcctgctgg cagcagtctt
     1561 gggggataac gtttttctaa ttcttccgga ctgggtaatt tttccggaaa cagtcttgcg
     1621 cttattgaac gcattttttc cataatgttg ttattttagc atgatattct aaaattacct
     1681 taaaaatcaa gaaaagtaaa gtttaaataa tatttgaatg attaattttc accctttctt
     1741 ttttcgcatt tttattgtaa aatgattata tcgcttagtt tttaaaaatt tttgttttgc
     1801 ggtataataa gcttaagata agccttaatt ttttctgttt tttatgtcta cagacgaaca
     1861 gcttttagcc gctcaaaata ctaagaatca gcctgatcaa gaaaattcaa gttctgaccg
     1921 ggcgggggct ttgcgtgaac aacaaagaga aggaggcggc caggaggaaa ccggtcaaag
     1981 cctgcgttct caagtcatgg ccgctagaaa ggatcaagcc agtgaagatg ccaagaagaa
     2041 cgaagagcgc aaaagcatca gttttcgcca cagccaaatc ttaaaacaga acatttttaa
     2101 tcttgttacc agttttggct tgagttattt ttatatagtt tatcattggt ttcagagtaa
     2161 gttgggtaat aagcgaaagt atgtgaaact aggttcggag tggatggata gtcccgctct
     2221 taccgagaaa cagcgtgatg aaattggtag taattttgcc ccctgggaaa attgttgttt
     2281 tttttctgtt tccattggtt gtttggtggc aatttttttg gtcatcctac cccttgcttt
     2341 gataggttat tatctttccc accccgctga agcggcttgg gaaggtttaa agtcagtttg
     2401 ggaaattatc aaatcaattt ttacatctta actatatgcg cttgcccgtg cgattttatc
     2461 ttaaacgcag attttggtcg gtttttttgc tctcaatcgt ttttttctct gtagctttgc
     2521 cagtatacgc caattggagc gataccttgg gtagcatcgt cggttggctt atcggcctgt
     2581 ttattagcgc gcttggcttg gttttgattc tggttattaa aggcttgctt ttaattgcca
     2641 gctatcagaa ttttattggt tccccggccg tgtttgaagg ttgggcgatt gttcgcgact
     2701 tgtccaacat gttttttgtt gtgattttat tagtaatcgc tttttcaaca attttgcatt
     2761 tggaaaatta tagttataaa aaatggttgc cgaagttaat tttgatggcc gtgctcatta
     2821 atttttccaa gactatctgc ggcttattaa ttgatgtcgc tcagattgtg atgctcacct
     2881 ttgttaatgc atttaaggat atcgccggtg gtaatttcat tgatatgctt ggcatcaggg
     2941 atatagttac catggcgaag aatacggatg atcttggatt ctgggctatc gttggcgctt
     3001 atgtcctcgg gcttatttac atgattgtcg ctgtagtggt gattgcgacg atgatgatga
     3061 tgttggtcat gcgcttagta atgatttgga tttatgttgt tttatctcct ttagcttact
     3121 tgttggccgc ttttcccggt ggtgctcaat attcttccaa gtggtggaaa gattttaccc
     3181 agaacttgat tgtcggtccg gttttggctt tttttatctg gttgtctttt gcggctttac
     3241 aaaacggcaa tgaattagat attgtgcgaa ctgctaagga taattctgtt aatgaggaaa
     3301 tagctgcagt cggggcgccg accaactcta aggagaagat gggttcgcca gtagcgatta
     3361 ctgaggcgtc tactcctgac gctttgatta aatttgttat tggtattggg atgttggttg
     3421 gtggtttgaa gatttctcaa gaaataggtg gcgccgccgg ttcaattgcc ggcaagggga
     3481 tgaataaatt gagtaagatg ggggcggcaa cgactggctt tattggcaag ggggctttag
     3541 gtattagtaa agacttggga atgggtcttg ccaaaaaagc aaaagttaaa gagggtttgg
     3601 gctatatttc atcaaggacc ggcgcagtgg gaagagcttt agcggttacc ggcatcagag
     3661 gtttggctac caaagggttg gtaagtttaa actctcaaga agcgcagatt aaggaaaagg
     3721 caaaaaagaa gttaagcaat atagacgata caagagttat ggctcgttat gccaatgaat
     3781 ccgcttttgg cggaatgggt atagcaatgc ggaaaaaggc gcgcacaaaa atgcccgatg
     3841 ctattgaaag tgatcaagaa atgaatgacg tcttggctag catgagcctc gacgacctga
     3901 aagaattatc agacgccaga tggactgctt taggcactag gcgtgcgcat ttaggtgggc
     3961 gttcaagaaa ttttattaat aaagactctg atgctcgggg cgcttttaat ttaggcatgc
     4021 aaagcgtcgg tgttcccgag agtgaattta ttgtcggtac tgatcggaac ggtgagccgc
     4081 ttgataatcc tgatagatat ggcagttata tgaatcctcg taatcttccg ctttccccag
     4141 atgaaattga tactttattt agtcgaggac gtcatcgttt tgattattat aaagatccta
     4201 ggcctattga agcggataca accgagaaag tttctccaga aaagcaaaat caagaaagag
     4261 ggaaaggcga tctggccatt aatggctttg ccgctggccg atcaacctta gcggtcgatt
     4321 ttgaccaatt aaatttagaa gatatcgaaa aaggttctgc cggtgataag gattggcgca
     4381 atgttcgcgg ggttaacact agcgacccgg ctatgattaa taaaattgcc agccgtttaa
     4441 cctctttaat agacggagaa atctcttctt tacaagcaaa gggtaatttg agcaaagggg
     4501 aggaaaagag attgtcgagt ttgaacgaag ctaaaaaccg tttgtctaat cctgagaatt
     4561 taagcaatct tgccttggta aactccagcg cctcccgctt caagatgagc gatgtcaaag
     4621 agacgattat tcatgaagaa atacacggcc tcggttacga agacgaagaa gaggtaagaa
     4681 gggctacctc taccattatg gccacccgca attatgatgc ccgtaaagac aaagcgtcaa
     4741 ttgatgctat tttgggtaag cagccgttag cgacgcgtcc ggtcgatcag ataatcgccg
     4801 ctgaaaaaga atctggtccg gtgtttgatg attctcccca aacggttgat tttgatcttt
     4861 ccccaataga acaaaagctt gccaattttg ctaagcaatt agagtctgcc ggttcgaaga
     4921 taagtaacct taaaatctcc ggggtctccg gttccgccag ccaggcgcct aattttattt
     4981 atttgttaaa agctcttaag aaatctttga ctaataaaga tgattcttca tcccggcaaa
     5041 tagcggcgct cgccagctct ctcggcgttc aaaaagcgga aactcccttg gaattccgga
     5101 tcatcgctaa taatcttccg gccgatttga agcctaaaaa caaggttgat caaagccaat
     5161 taaattaata attttatgaa ttatatcatt gagagcttga ataaattcta taatctgcca
     5221 atggatataa ggcaaaaaat agggggcgtc gactccatgc tggtcgttca agatattgaa
     5281 aaaaaatacg gcgtcgatat cagttttgcc ttgattttaa tagcgatagg ggaactgaac
     5341 gctaatgact tatccgctta tctccaaaaa aaatattttt tgtcggccga cgatgcttac
     5401 gatatagcga acgaagtgac ccgcaagatt tttgtccaat tgttttatga cgataacgat
     5461 aaaactaacc agtctttatt gattgccgaa ttgaacgaag ggttgtttaa tctcttaaaa
     5521 gatcaggcca gggcttcaat ttttaataat aatgttttta aaattttagt taatgatgct
     5581 tcttttctgg aaagattggg aaaaagctta ttgacgaatg aattaattat cggtaatgcc
     5641 caaattttat cttctggccg aaaagtgaaa cccacggttg ctaattggat taaagatttc
     5701 ttaacaataa atggcaccga taattttaac gacatagatt taattcgtta tattactgat
     5761 gctgataatg ccaaaaatct ttctaaagaa gacaaatccc tgctttttaa agtgttaaaa
     5821 acctatgcta atattgcctt tttcttagaa aatagcgaga aaaaaccaat aagctcgctg
     5881 gagtttattc ccaccggcac cactaataat agcgacaatg attttttatc tgatcaggcc
     5941 gatgataaag cgattttaaa agatggagcg gataaagaca ctgtccaatc tccagccaat
     6001 gtttccgatt tagattattt acagaagatc ttgccaaatt ataattataa tagtttagag
     6061 tataaggcga ttagtcagga aatagaacgt ctgcgtaaaa ctaaaaaata atattttttt
     6121 gtaaggaaaa ttaagttatt tacagaaaaa gaaaagccat ttttgaaaga ggcccagaga
     6181 gcgggttgtt atatttatta tgtctttagc gctcagtttt ggtacttttg ataatttcca
     6241 tcccgggcac gccgtttctt tacagctagc ctctttttgg ggcgatgaac tccttattgt
     6301 tattgcccga gaggttaaag gcggaacggg aaagattagg cgcaccggcg cttttctttt
     6361 ttgttaggag aaaaaataga gggtaaaact gttttcggtt ttttaaaggc gagcgtttat
     6421 tagtattaaa aaagtttttt aggtaatttt tttataaata aatttgatat aactcctttc
     6481 ttgatagcat gctcattttt gtggtaacca tagatttgaa ccttaattta tagattttat
     6541 ggttacattt tacaatcatt tgtcgaatag ctctatggtt acattttgca tcatttgatt
     6601 taacctagtt gctttttatg ttgtaatata ttacaatata tgttgtaata atttacaatt
     6661 atgattaatt ttaattctaa taaaataaca caaatattag cctttttttt cttaaatcca
     6721 aaacgcagat tatatttaaa agagctggca aatgttttag aaattgataa tggaaattta
     6781 tctcgttatc ttaaatcctt ggtagaagag ggaatattga aagttgaaat tgaaggaaaa
     6841 caaaaatatt ttttattaga ttcaagttat ccttttttaa aaaacttaaa aaagatattg
     6901 acgggcagtg ttagcccgga agttctaatt aaaaaagctt tggcgccaat taaaggttta
     6961 gagcgggctt atttgtttgg ttcgtatgct tccggaaaat taaaggaaaa tagtgatttg
     7021 gatatattat taatcggcag ccatgattct atagaggtta gggaagtttt agcttcatta
     7081 caacgtcgtt tagggcggga aataaatatt attgattatt cggtagagga gtatgaaaaa
     7141 aggctgaaag aaaaagacga ctttctggac agggtttttc atgaatctat gttaacttta
     7201 aaataacatg tttgttaagt ttatcttttc taaacaacaa atagataaat attttcaggc
     7261 ggcggaaagg gatttatttt tagcggccaa caaagaaccg gaaataaaat ttcagttttc
     7321 ttataacagc ctgttaaaat tagctcaagc cgtttgtgct aaacaaaatt tgagagttaa
     7381 agcgcggaca ggccatcata tggttctttt tgataagtgt gcggaattat tagatgatag
     7441 aaagattgcc gccgtagcgc aggcgatgcg tgataagaga aatcgtgatt tgtatgatgg
     7501 cggtacaatt ataactataa aggaagcgga aacttattac atatttatta aagatttggt
     7561 taaaagagta aaatcatatt taaattccag attaataaaa taaaatttat atctgcaaac
     7621 aagataaaac tcatcacttc cgaattagtg accgcggacc atcctgagaa gatttgtgac
     7681 cagattagcg atggcatctt ggcggcgatc ggtgtggccg aaccgggggt attcatttgg
     7741 aaacttttgg taccaataaa atagaagaag ataaaatatt agaagcggtg aaagcaagtt
     7801 ttgattttcg gccgccagcg attattaatc aattagaact taaaaggccg gtttttaagc
     7861 agacagctgc ctacggacat tttggcaggc cggaatttac ttaggagaag attgataaag
     7921 cggaactttt gaaatcatct ttaaagcttt aaattaatgc ggtttttgcc gcatttttaa
     7981 atatccatta tgtcttcagc cctcagtttc ggcgctttaa ataattttca tcccgggcac
     8041 gctgtttatt tacagccagc tacttctttg ggcgatgagc ttttgtagtg atggctagaa
     8101 atgttaatgt ggaaaggata aagaataaga aggcgcggca aacggaagat caaataagaa
     8161 aaaaggtgac ggaattttta cgacaacaag gaattaaagg aaaggctgtt ttcggttatg
     8221 attagccagt cgatgaggat caattacgtc gagagttaat ttcactgagt ttaaaaacga
     8281 aaggcgaacg tatggcccct tattatcctg agaagtgtaa gtctagtttt ttaggtaatt
     8341 tttataaata aatttgatat aaatttatta tctcagccac cattttagcg tggttttttg
     8401 attatttttg tggatatttt ttattgtttt gtcgcttttc aatttttggg aaaatatgct
     8461 aaaatgaaga taatgcccga aacagatttt gttggttttt atttttagtt tttttctttc
     8521 attcatgttt gattatttgc agcaattcaa taaactgccc aaggagttaa gggagaaagt
     8581 ttcctcgccg gcggcgatgg cgattttgtc gtctttagaa aataaatacg gtgtcagttt
     8641 ggcgatgcta gtcatacaga taatgataaa acaggtttta attaaagatt tagtgcctta
     8701 tttaattgag gaaatgagcc taactcctga taaagcccaa tctctgtcac aggaattaca
     8761 agagcgtctt tttttctcgg tagctactta tcttggccta aagacacccc cttctttaaa
     8821 cccagaagag aaagaattac aagttttaat gaaagaaaat agcatcattt tgcctagtca
     8881 ggagctgatg aatcgttgtc gacagatatt attgacttat cgtaagggta ttaggacaaa
     8941 aattgatgtt cgcgctgctt tagaaaaacc agtggcgcaa ggaggtttgg gattggaagt
     9001 ggcagtagtt gataggttgc ttcggtcttt agaaaaatcg ccggtagcca cgtctttgcc
     9061 tcaagcttcc gccgctttca cagatttagt caataagaat gaagccagtt cttatgattt
     9121 aaagtcagct cttgcccgcg gcgctgttaa gccgccggca gaactaatgt ctaaatttaa
     9181 gtcgccggcg gctaaactta acttacagcc tgaacttgaa gctccagaaa aaactttagc
     9241 cttagaagat aaagggactg ttaaagaaaa tttacctctt agcgttcagg aaaaatctnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt ggaaaaatca aattcccagg
     9421 ttatgtctcc ccagccgcca gcggcgccag cagaaaaaaa agaagttgaa gctaataaat
     9481 ttgctccggc gcctaaatct cgtctttggt caaagctttt taaagctcag gaaaaaaagg
     9541 cggagatgcc tttaaaaaag attagcgttt ctagtgacca cttagccgaa gccgtgaggg
     9601 cggcagcgca agcagccccc gcttctgtcc gccccgccgc ttctatagag acaaaaacaa
     9661 aaatggaaga tgtcagggcc cgtccgaaag tgatgggacc cttagaagaa ttaagatatt
     9721 tagacttggt caattttaga agattgggcg atagccctaa agcaataacc gataagatcg
     9781 tgatgaagat taagctctta gagaaagacg gctacgaccg catggttgaa ggcgttcagt
     9841 cttggcgtca gagccctgtg aatcgtatat atgtgcgctt ggcgcaagag gcggtggcta
     9901 aaggaattac tttgcgcgat gccgttgccg cccgtcaagc ggccaataag gaaactttaa
     9961 gcatggagga aattgaagcg attgtcgcca tgaatagccg tttaatgttt taatatgcag
    10021 caatttactg ttcctcaatt tattgatgtt gagagcaaaa ttatcgggcc gattactacc
    10081 cgccaatttt tgatattttt gggagcggcg gtgattatcg gtattagtta taaggttttc
    10141 gatttttctt tgtttttaac gattggcatt atcattttca ttatcgctgg cgtcttcgcc
    10201 tttttaaaga ttaacggccg ccctttccat ttgtttttgc ttaattttat tcagacaatg
    10261 cgtcgcccct ctctgcgggt ctggaatcat caattaggat tcatagacac gccggcgccg
    10321 atgaatattg ttaaagaaga agcgccagta aagaaaaagg aattttatca aacttcgcgt
    10381 ttagccgatt tagcgctggt ggtggacact aaagggcgtt atcggggcga tggggacaat
    10441 aacgaaacag tgtaaattta tgcctgctaa aaaaaatttg gccagcaata aaataaacgt
    10501 ttccacccag caatatcttg atattgcgga gattaaagat gatgtcgtta ttatgaagga
    10561 cgggactttg cgcgccgtac ttttggtttc tagtattaat tttgccttaa agagcgaaga
    10621 tgagcagaat gccgttatta attcttatgt catgtttctg aataatcttg atttcatgct
    10681 ggaaatcgtt attcaatcac gggaattgga tattgataac tatcttaatt atttaaagga
    10741 gaaagaaaaa cagcagatta ataagctttt aaagatccaa acggcggaat atattgagta
    10801 tattaaggaa ttaaccgcaa tgggaaagat tatgaacaaa cggttttacg tggttgtccc
    10861 ctataacccc ttaaccgaca aacataaagg tttttttact cttctaggag aatcgttgaa
    10921 gcctgcgacc tttattaagc taaaagacaa gagttttgcc cgctataaag aaatgttgaa
    10981 tcgccgttta gacagcgtca tcggcggctt agagtcgatg ggtgtcagtg tcgcccgttt
    11041 ggatacacag agtttaattg agctttatta taagacttat aatccggaaa cagccaagaa
    11101 tcagagtttg gttgatttgg aaaaattacg gattgaaaca aattaattag cgtttatgat
    11161 aaattttaat cagaatgagg ataaacaaaa aataggcttg aatacgaaaa tttctttgtc
    11221 gccatcaggc caggggcagc cgattgaagc caatccggtt ttgaccaaag aaatcatcga
    11281 ggaggaaaaa gcttttcgcc gcggcgtttt gacggtgcgt gatattattt ctccggccag
    11341 tttgcaggtg acgcccaagt atctccgtct cggctccaaa tttgtccgca ccatttttgt
    11401 tattggttat ccccgttata tcagtgttgg ctggtttgct ccgattatca atcttaattt
    11461 aacttttgat atttccatgt ttttctatcc ggttaagagc gcgatcattt taaagcagct
    11521 gaagaacaag gttggcgttt tggaagcgca gataatttct gatgcggaaa agggagcggc
    11581 tcgcgatcct ttgcgggaaa cagctttacg cgatattgaa tatttgcgtg acgcccttac
    11641 ccaaggtaca gaaaagtttt ttcaattttc tctttacgtt accctatact cagacagcct
    11701 ggaagaattg gataggatgt ctgaccaggt ggaagatatt ttcggctctc gtttagttta
    11761 ttcgcgtcgg gtttattttc aggcagaaca aggatttaat tctaccttac ctttgggtaa
    11821 cgacgagctt tatattactt ttaacatgaa ttcctcgccg attgcttctt cttttccttt
    11881 tatttccagt gaattgacca gtgatcgcgg aattttatat ggcattaatc gccataataa
    11941 cagtttgatt ttattcgacc gcttttcctt acaaaatgcc aactctgtcg tgtttgctac
    12001 ttccggcgcc ggtaaatctt atgccatcaa gctggaaatt ctccgttctt tgatgctggg
    12061 ctctgaaatc atcgttatcg acccggaata tgagtataag catctggcag aagcggttgg
    12121 tggcacttat attaatattt ctttagcctc agaaaataag attaatcctt ttgacctgcc
    12181 tcgagctatc ggtggcgaag ctaagcctaa agatattatc cgtagcgctg tcatcaccct
    12241 taaaggctta gtgcgtttaa tgctgggcaa gcttaattat gacgaagact caatcgttga
    12301 ccgcgccctt ttagaaacct atgccaaaaa agacattact ccggattctg atttatctac
    12361 cattgaagcg ccgataatgt ctgattttga acaagtttta gacggtatgg agggcggggc
    12421 ggatttagct ttacgcttga aaaaatatac ggaaggcact tttgccgggc tactaaatag
    12481 cgttactaat gtggagatga ataatcagct ggtttgtttt tctgtgcgtg atttggaaga
    12541 tgagcttagg cctttagcta tctacaccat cgttaattat atttggaaca tagtacgcag
    12601 cgagatgcgc cagcgtattt tagtgattga tgaggcttgg tggatgatgc agaatgaaga
    12661 cagtgccaaa tttatttttg cgttggttaa acgctgtcgt aagtattatc taggggtgac
    12721 taccatcact caggatgtta atgatttctt gtcctctccc tacggccagg caatcgttac
    12781 caattcttct ttgcaaatat tgttaaaaca gtcgccggcc gctatcgacc ttgttcaaaa
    12841 aactttcctc ttaaccgaag gagaaaaata cctcctccta gaatgcggag tcggtgaagg
    12901 tattttcttt gccggcaata agcatgtagc gatgaaagtg gttgcttctt atgccgaaga
    12961 tcagctgatt acctccgatc cgcgtcaatt attggaaatt gaagaggcta aaaaagaatt
    13021 taacgaaagc ttagccgaag ggcaggctta aataaaaata tatggacaag cgtcaaaagt
    13081 taggactatt aattattgtt atcggcttgg caattattgc cgttattatc tattttattt
    13141 ttattcgcca agcggaagtt ccggctacga ccatagcccc gccgacggcg agcacgaccg
    13201 taccgttgcc aactgagccg gtagagagcg atatcactcc tagtgataag ccccgtaatt
    13261 atcagcagta tgatatttcc aaggagccgg agcatcaaac tgacgctgcc gatgtggcta
    13321 aactttccgc cgcttttgcc gagcgcttgg gttcgttttc taatcaatca gattatagca
    13381 actttgaaga cctagattta tttatgaccg cgtcaatgcg cgactgggca aaatcctacg
    13441 ttgctaagat gcgcgccgac aatccctata acggttccta ttatggtatt accactaaag
    13501 ccatcagcgc ggaagtaaaa agttttgatg acgataaagg aacggcggag ataattgtgg
    13561 gcacccagcg tcgtgaagtt ggcgccgacg gtggcgaacg caattttcag caggatttgc
    13621 gtttgacttt tttgaaaatt aacgaccaat ggctggtcga cggcgcctat tggttaaaat
    13681 aattatggcc atcaagaaga tttatgtttg ttcccactgc gacgcccaat ttcctaaatg
    13741 gagtgggcgt tgcttagaat gcggttcttg gggaacatta gttgagaata taaccgacgc
    13801 ccagacggag aaaaaggaag aagcaaaaaa attaggtgac gccaaggtga ttgatctaag
    13861 gaccttaaaa ggcggtccgg aaataagaat taagagcggc tgggaagaaa ttgatcgtgt
    13921 gctcggcggc ggtcttttgc ccggttcttt attattactc agcggtgagc ccggaatcgg
    13981 caagagcact ttggcggcgc agttggcaga cgctttaagc aatggccatg aagttttata
    14041 tgtgagcggc gaggagtcgg ccttgcaggt aaaggcgcgt ttggagcgct taggttgtga
    14101 tttgtcccgt ctacgtttta ttggcgagac tagcgtggaa aaaatcagca gtgccgctct
    14161 gaaattgagg ccagccttat tagtcattga ctctattcaa actgtctatt cttctttaat
    14221 accctttgaa ccgggcagtc tcagccaaat tagggcggtg gcggtaaaat ttctggaaat
    14281 tgccaaagag tataatattt cggttttgct tatcggtcat attactaaag atggccagat
    14341 tgccggaccg aagtctttag agcatattgt tgatacggtt ttgtctttag aaaatgaaac
    14401 cggcagtaat tattgccttc ttcgagccag taagaatcgt tttggttcgg ttaatgaatt
    14461 aggagtttta gaaatgaccg gcaagggttt tagggaagcg aaagatccaa gttcggtttt
    14521 tctggggcaa ggaagctcaa tgattgccgg gtctgtcgtc ggctgtgttt tagagggtag
    14581 tcgccctttc ttggttaaca ttcaagcctt agtgacaaag acagtcttcg gttatccgca
    14641 gcgccagacc agcggttttg atttgaatcg cttgcaagtg cttagcgcgg tgattgctaa
    14701 aagaagtaag attaatttgg ttacgcgcga tattatttta aatgtggtcg gcggtttaaa
    14761 aattaatgac ccggctttag atttagcggt gtgtgcggcc attatttcct cgtatctgaa
    14821 taagccgctg ccggccggta tggcgacgat tggcgagctt ggactgggag ccgagcttcg
    14881 tcctgttttc cgtttagaac agcgcttaaa agaggcggag aagttaggtt ttaagagtgc
    14941 gattattccc gaaggagctg agtataaatc aggtaaatta aagcttttaa agataaataa
    15001 tttaggagaa ttagtagaaa tattagctta aatttttaaa aatgagaata ttttattgtt
    15061 cttttgattc gtttctcttg atttaatgac ctaaataata tataattagg ttattataat
    15121 gacctaaata atataa
//
LOCUS       DAOQSA010000021        13789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_3513_length_13789_cov_7.733945, whole genome shotgun sequence.
ACCESSION   DAOQSA010000021 DAOQSA010000000
VERSION     DAOQSA010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13789
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3513_length_13789_cov_7.733945"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..1133
                     /gene="pyk"
                     /locus_tag="PKK65_02980"
     CDS             102..1133
                     /gene="pyk"
                     /locus_tag="PKK65_02980"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNU81674.1"
                     /translation="MPTKIIATIGPKSESKEKLLALLKAGVDMVRVNFSHASHSQYLR
                     IQKIIKDFNAESERQIKIMLDLQGPRIRVGRMPEGGIFMVDGESYRFAFSNKPYVKAG
                     DVIPIDSHDLYQDIKKGEALFLCNGAIELQVKAIKNKIITAEVINGGVLSSRKAINVP
                     DTHIRKGGMTAKDLKDAAFGLKVGVDYIALSFVQTANDVLRLRRLLGKKSGIKIISKI
                     ERGLALKHIDKIITSSDAIMVARGDLGIEVPIEDLPIIQKNLVRHAHWHDRPAIIATQ
                     VMTSMIKNAHPTRAEISDIANAVLDGADMIMLSDETTIGDYPVEAVKILKKVIERTEK
                     YIYKGNVDN"
     gene            1158..2447
                     /gene="eno"
                     /locus_tag="PKK65_02985"
     CDS             1158..2447
                     /gene="eno"
                     /locus_tag="PKK65_02985"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNU81675.1"
                     /translation="MKIKSISALEILDSRGNPTISAKVTLEDGSVGEAAVPSGASTGS
                     YEAVELRDGDINRYQGAGVLTAVKNVEEIIAPALIGFEADNQVGLDEKMIALDGTTNK
                     AKLGANAILSVSLACARAAAVSSGQPLYQYLSKFNPEFNGQYILPVPMMNVLNGGKHA
                     NWATDIQEYMILPVGAKNVAGAIRMGAEIYQSLKKILKNKGYAISVGDEGGFAPAFKD
                     NEEPFQVLTEAVVATGYSLGEEVVFGIDAAATEFYTDGVYSLKKEGKKLSAQELIFFY
                     QELSARYPLVSFEDVLAEDDWDGFRAFTEMFAPLCQIVGDDLYVTNVKRLERGIAEKS
                     SNSILIKLNQIGTLTETVAAINLARKNGLTTVVSHRSGETEDAFIADFVVAMGMGQIK
                     TGAPCRSERTAKYNRLLAIAAELGDKAVYAFFPFAGK"
     gene            2477..3577
                     /locus_tag="PKK65_02990"
     CDS             2477..3577
                     /locus_tag="PKK65_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNU81676.1"
                     /translation="MKSIFFTLNVIIDLAGHFLMFLIWRHFFGFSELYLQVTVGVILL
                     GAIFLTVLAPLLVYWRDNIWSRSLYLVVGLWEGLMLNSVLLALIFIVFDFFGVYEISS
                     WSPGVRWWWLGALPLLLLAPEAWLAQATRIRRVSVNIINLPDSWQGKEIAHITDVHLG
                     PVWRQRFFDKLVHRVNSLEAEAVFITGDLFDGMDTDFSWFHQRQFVARKGVFYALGNH
                     DLILGEARLRTLLANSNIQILSNEIVTVDNLQIVGLNCYYEGRLDVKGKILSQLGYES
                     DKPSILLYHEPKDLEAAREAGIDLQLSGHTHGGQMFPFNLVAKILYRGFSGGLYHLKN
                     FSLSVSFGVGTWGPPLRLGSRSEITLIKLLKA"
     gene            3599..4606
                     /gene="rlmN"
                     /locus_tag="PKK65_02995"
     CDS             3599..4606
                     /gene="rlmN"
                     /locus_tag="PKK65_02995"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNU81677.1"
                     /translation="MDLSKLSGILQTEPKFRFRQVYQALYGELISDWAQASVLPLNLR
                     ERLNQDCPLDITAEISVDPGDKSSLKALLTWADGVSVETVLMRHRDGRHTICLSSQAG
                     CPLGCAFCATGQGGFFRNLSADEIVQQFIFWARFLKQENKGESINNVVFMGMGEPFLN
                     YEQFIKAVKILNDPEKFNLGSRRMSVSTVGIIEGIKKLSTEKLQVNLAISLHAAVDSL
                     REQLMPIAKKYSITSILKAVDEYIRKTGRRVMFEYIMIKNVNDGEDDALALAQLMKKP
                     LYLVNLIPYNPTGKFQASSPARIKEFKKKLESLGVAVTERHSQGRGISAACGQLAGKK
                     K"
     gene            4874..5470
                     /locus_tag="PKK65_03000"
     CDS             4874..5470
                     /locus_tag="PKK65_03000"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017721126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNU81678.1"
                     /translation="MFTLKSLPYAYNALAPHISEQTMRLHHDKHHQTYVDNLNKLTEG
                     TSLAAMSLVDVIKVSAGQANKAGIFNNAGQVYNHDFFWDCLRPADQVMAIPAALLEKI
                     NHDFGSLEQMMVEFKTIALSQFGSGWAWLVLAGESLQIIKTANADTAFLHDVNPLFTL
                     DVWEHSYYLDYQNRRGDFIDAVLNNLVNWDFVANQAGL"
     gene            5473..6150
                     /locus_tag="PKK65_03005"
     CDS             5473..6150
                     /locus_tag="PKK65_03005"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNU81679.1"
                     /translation="MMKKNPYARLQPKTREPYTVIYLVRHANPDYRLEKKLGDRLMPL
                     SKEGKLQASFLAKRLLKLDLDTVYASSLARAQETASIFANRRKKRPIIIDERLDEIDW
                     KNWHRVKYFRTSEERRKQYLPNYHVLDKQLDKMQARTRRFLAEVLAEHRGQRVALFCH
                     GNIIKTFLTGIMNADVLGFLSLEIYQASISKLVIDGDGYVKIAYINDINHLPNPPLED
                     IFITLVS"
     gene            6155..7627
                     /locus_tag="PKK65_03010"
     CDS             6155..7627
                     /locus_tag="PKK65_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81680.1"
                     /translation="MYFLRLLKFITIPLLSAFFLYYNALPVKAETLASRLSGKILLQV
                     ESAGEAWYVNPLDGRRYYLGRPADAFVLMKRLGLGISEQNYQLFKETGAHNLRGRILL
                     RAQASGEAYYVNPDDSRLYYLGRPTDAFNLMRRFGLGISNQNLNQIVVADLSSAVNDL
                     KANTGTYSWQYQNTKYQLSFNLDSALYQSYASSPKVYTYYVGQEPADIKEAFYALFLK
                     VKSNDNQTLGLLNSLRAQAASLGLNSDQTAAFILAFIQYIPYDHAKLESGDNTPYYPF
                     ETLYLHKGVCADKTFLAVLWLRSLGYGAAILDFPESNHSAAGIACPVADSLAGSGYCY
                     VETTNYFPIGVVPPLITGGQAQNDTSSFEDLFNSDHLGYLEIKQKSQGKIYQGVAAVK
                     REARAIEQAKTDLDASQNLLNANKLSLDAAYQSLKQQESQLLAYQAAGNISAYNQLVP
                     IYNQAVAAYRDQTESYNQSVVSYNQAVNAFNDRYRQFYQQ"
     gene            7858..8553
                     /gene="rpsB"
                     /locus_tag="PKK65_03015"
     CDS             7858..8553
                     /gene="rpsB"
                     /locus_tag="PKK65_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNU81681.1"
                     /translation="MVKLPSLEEMLKAGMHFGHRTHRWHPKMKPYIFTAKNGIYVIDL
                     EKSRQMLEEALNFMSQLVREGKSILFVGTKSQVKAPMKKMAEMIGQPYIVGKWLGGYL
                     TNFAVVKKSVKKYQDLIEKRDAGKLEKYTKKERLNFDREIKRLAERVGGLTSLNKMPD
                     AIFVWDIKEEETAVAEARKRRIPVIAICDTNVNPELVNYPIPANDDATKTVKLILEVI
                     QSAIIEAKKDLKN"
     gene            8605..9399
                     /gene="tsf"
                     /locus_tag="PKK65_03020"
     CDS             8605..9399
                     /gene="tsf"
                     /locus_tag="PKK65_03020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNU81682.1"
                     /translation="MEIIKQLREMTGAGIVDCKKALDEANNDLRVAVEILRKKGIAKA
                     AKRIDRETNEGVVKVAVAAAAGEAYIFELTSETDFVSRNDKFQKFADEVMDLVLSLRP
                     ENLEALLSLPMAESNVKDNLAVLSGTIGEKMEIRRFARLHGASLAAYSHLGGKLGVLV
                     ALDQAGKEELAMTVAMQVAAANPKYIEPSEVEAGELEKEKEIYREQLLKEGKPEAMIE
                     KILEGKVTKYYSEVCLVEQEFIKDDKKKVKDILGGAKILGFVRFSL"
     gene            9467..10345
                     /gene="ricT"
                     /locus_tag="PKK65_03025"
     CDS             9467..10345
                     /gene="ricT"
                     /locus_tag="PKK65_03025"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNU81683.1"
                     /translation="MQIAQVQFSPWDKAYSFYVDSSLGVLVGDFVIVDTELGRELGKI
                     ISVSKESSPEQDLKLVLRLATVDDARLVFDEDRRRQALVTCQGFIERHGLNMKLIDVR
                     FSFSGNRMTFAFVAEGRIDFRDLVKDLNAAFNANVRLLQIGSRDEARYCGDCGPCGLS
                     LCCRTFVHNFASITSEMAEAQQVVHRGSDRLSGMCGRLMCCLSFEYEGYKQLAKELPP
                     LGTKIRVQGRTGIVVGHHLLKKTVDVQFEAENEGERAFVIQVDPNQNKEKELKPRIGH
                     FAKFRANGNTRPRPRR"
     gene            10442..11452
                     /gene="gap"
                     /locus_tag="PKK65_03030"
     CDS             10442..11452
                     /gene="gap"
                     /locus_tag="PKK65_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020599231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNU81684.1"
                     /translation="MPLNLAINGFGRIGRAAFKIILDKYPELKVVAINDLTDNQTLAR
                     LLRYDSCYGLYGHRVSGDDQGLLVDGQRYTVFAEKDPAALPWKKMQVDVVLECTGHFC
                     DKEGAGMHLQAGAKKVIISAPAKKGGVKTIVLGVNDKDLRASDHIISNASCTTNCLAP
                     AMSVLQEKFGVLKALMTTVHSYTADQNLVDGPHKDPRRARAAALNIIPTTTGAAVAVT
                     ETLPSLKDKFDGLAVRVPTPVGSLCDIVCVLKKKTDVTTVNKAFLAAAKGKLKGILEA
                     SNEEIVSTDIVGNAHSSIIDLKNTKVIAGDLLKVVAWYDNEWGYANRLVDLAVKLKKF
                     VK"
     gene            11497..12234
                     /locus_tag="PKK65_03035"
     CDS             11497..12234
                     /locus_tag="PKK65_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81685.1"
                     /translation="MTNKISSKVNSPLLPATRLLTDAWNLYAYKLVDFIEMYVLGLFG
                     LLPLLLLGILALVFFAWMHLNSWTIYLLFGVLGLAALAWAIYYGTRAKIGLLLILKNE
                     KAKVRENFQASKSLFFPYFFISLATGVLIFLAFLLFIIPGIILSVFWSLVVILIVFED
                     KLTFKTATKRSCELIKGYWWPVFGRFLIIGVLATIVALIMNIPMNYLSEIGKQAYSFV
                     VNIFWALLSPLFVTYTYLLYKDLLSKK"
     gene            12259..13725
                     /locus_tag="PKK65_03040"
     CDS             12259..13725
                     /locus_tag="PKK65_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HNU81686.1"
                     /translation="MMIPDYILEIMERLMSAGFSVEIVGGAVRNLLMNKQPKDWDLTT
                     QAKPPEILSLFPEAKYENDFGTVIIPCRTSDGSLSDVVEVTTYRSEQAYSDRRHPDEI
                     KFENSLEKDLERRDFTINAMALILDKAGEYEWSGRHFRLVDLFGGLKDIHKKVIRAVG
                     EPSDRFKEDALRMMRAIRFSAQLGFSLEPKTQRGVVKLAGSLKFVAKERIRDELIKII
                     ASNQPAEGIAALHEAKLLQYIIPELEQGVGVRQNHHHIYPVFKHNLLSLKYCPSQEWT
                     VRLAALFHDIAKPKTRRLINGQVTFYNHEYVGAKMVDRIMTRLKFKNEDREKVVNLVK
                     NHMFYYNVGEVSAASVRRLISKVGRENLQDLIYLRVADRLGSGTPKAMPYNLRHLQYM
                     LEKVQNDPVSVKMLAINGDDLISVLKLTPGPKFGAILDVLLGEVLEDPARNERSYLLA
                     RAEKIKEQNLDEIREQARKLIEEKRDDDDQSLQRRFKV"
BASE COUNT         3739 a         2405 c         3201 g         4444 t
ORIGIN      
        1 attttttctt tttttgtttt ataatgctgt tgctaaacaa attatattta tataaataaa
       61 gagggtggct tggctcgcta tttttatttt ttcctcgatt tatgcctaca aaaattattg
      121 ccacaattgg accaaagagt gaatccaagg aaaaactttt ggctctattg aaagctggcg
      181 ttgatatggt gagggttaat ttctctcatg ccagtcatag ccagtatttg cgcattcaaa
      241 agataataaa agattttaac gccgaatcag agcgtcagat aaaaataatg cttgatttac
      301 agggcccaag aattcgtgtc ggccgcatgc cggaaggcgg catttttatg gtcgacggcg
      361 agtcttatcg tttcgctttc tcaaacaaac cttatgtaaa ggccggcgat gttatcccta
      421 ttgacagtca tgatttgtat caagacatta agaaggggga ggctttgttt ttatgtaatg
      481 gtgccattga gttgcaggta aaggcgatta agaataagat tattacggct gaggttatta
      541 atggcggtgt tctcagttct cggaaggcta ttaatgtccc tgatacccat attcgcaaag
      601 gcggaatgac agccaaagat ttgaaagacg ccgctttcgg cttaaaggtg ggggtagatt
      661 atattgcctt atcttttgtg cagaccgcta atgatgtttt gcgtttgcgc aggttattag
      721 gcaagaaaag tggaatcaag attatttcta aaattgaacg tggcctcgct ttgaagcata
      781 ttgataaaat tattacctct tctgacgcta ttatggtggc gcgaggagac ttgggcattg
      841 aggttccgat tgaagatttg cctattatcc aaaaaaatct tgtccgtcat gctcattggc
      901 atgatcgtcc ggctattatt gccactcaag tgatgacttc catgattaaa aatgcccatc
      961 cgactcgagc ggaaatttcc gatattgcta acgccgttct tgatggcgcg gatatgataa
     1021 tgttgtctga tgagacgact attggcgatt atccagtcga agcggtgaag attttaaaga
     1081 aagtgattga acgtacagag aagtatattt ataagggtaa cgttgataac taaaatttag
     1141 atattaatta tttttttatg aaaataaaat caatttccgc tttagaaatt ttggattctc
     1201 gcggtaatcc cacaatttcg gcaaaagtaa cacttgaaga cggttccgtc ggggaagcgg
     1261 ctgtgccctc gggagcctct accgggtctt acgaggctgt agaattgcgc gacggtgaca
     1321 ttaatcgtta tcaaggagcg ggggtgttaa cggctgtaaa aaatgttgag gaaataatcg
     1381 ctccggcttt aatcggcttc gaagcggata atcaggtcgg tttagacgaa aaaatgattg
     1441 ccttggacgg tacgactaat aaagctaaat taggggctaa tgccattctt tcagtttctt
     1501 tggcctgtgc tcgggcagcg gccgtatctt ccggtcagcc tttgtatcaa tatttatcta
     1561 agtttaatcc tgaatttaac ggtcaatata ttttgccggt accgatgatg aacgtattaa
     1621 atggtggcaa gcatgctaat tgggcgacag atattcagga atatatgatt ttaccggtgg
     1681 gagcgaaaaa tgtcgccggc gcgattagga tgggggcgga aatatatcag tctttaaaga
     1741 aaattttaaa gaataaaggt tatgctatca gtgtcggcga tgagggcggt tttgctcccg
     1801 cctttaagga taatgaagag ccttttcagg ttctaacaga ggcagtggtc gcgaccggct
     1861 attctttagg cgaagaggtt gtttttggta tagatgccgc cgccactgaa ttttatactg
     1921 acggtgttta tagtctcaaa aaggagggta agaaattgtc tgctcaggag ttaattttct
     1981 tttatcaaga attgtccgcc cgctatccct tagtgtcttt tgaggatgtc ttagccgaag
     2041 atgattggga tggtttccgt gcttttacgg aaatgtttgc gcctttatgc caaatagtcg
     2101 gcgatgatct ttatgttact aacgttaagc gtttggagag aggaattgcg gaaaagagca
     2161 gtaattcaat tttaattaaa ttaaatcaga ttggcacctt aacagaaacg gtggcggcta
     2221 tcaatttggc tcgtaaaaat ggtttgacga ccgtcgtctc tcatcgtagc ggtgaaaccg
     2281 aagatgcctt tattgctgac tttgttgtgg ctatgggtat ggggcaaatt aagacagggg
     2341 ctccttgtcg cagtgaacgc acagctaagt ataatcgtct tttggcaatt gccgcggaat
     2401 tgggagacaa ggccgtttac gctttttttc cttttgccgg caagtagttg cttgtattgc
     2461 aagcgcggcc ttttttatga aatccatttt cttcactctg aatgtcatta ttgatttagc
     2521 cggccatttc ttgatgtttt tgatttggcg gcattttttc ggtttttctg aattatattt
     2581 acaagtaacg gttggcgtga tcttgctggg cgctattttt ttaactgttt tagccccctt
     2641 gcttgtttat tggcgtgata acatttggag ccgttctttg tatttagtcg ttggtttgtg
     2701 ggagggatta atgctgaatt cggttttatt agcgctcatt tttattgtct ttgatttctt
     2761 tggggtttat gaaattagct cttggtctcc tggggtgagg tggtggtggc tgggggcctt
     2821 gcctttgctt ttattggcgc cggaggcctg gttggcgcaa gctaccagaa taaggcgagt
     2881 atcggtaaat attattaacc tgcctgattc ttggcaggga aaggaaatag cgcatattac
     2941 cgacgtccat ctcgggccgg tttggcggca gagatttttt gataaattag tgcaccgagt
     3001 caactcttta gaggcggaag ctgtttttat aactggtgat ttatttgacg ggatggatac
     3061 tgatttttcc tggtttcatc aacgtcaatt tgtcgcccga aaaggagttt tttatgctct
     3121 tggtaatcac gatttaattc taggcgaagc tagactccga actttattgg caaattcaaa
     3181 tatccagatt ttaagcaatg aaatcgtgac agtggataat ttgcagatag tgggcttaaa
     3241 ttgctattat gaaggccgtt tggatgtaaa aggtaaaatt ctttcccaat tgggttatga
     3301 gtcggataag cccagcattc ttctttatca tgagcccaag gatttggaag ccgctcggga
     3361 ggctggcatc gatttacagt tatcaggcca tactcatggt ggccagatgt ttcctttcaa
     3421 tctcgtggct aaaattttat atcgcggttt cagtggtggc ctctatcact taaaaaattt
     3481 ttctttatca gtcagttttg gcgtcggtac ttggggaccg ccgctgcgcc tcggtagccg
     3541 ttcggaaata accctgatta aattattgaa agcttaacta gtccttgata tttatattat
     3601 ggatttatct aaattatccg gaattttaca gacggaacca aaatttcgtt ttcgacaagt
     3661 ttatcaagct ctttatggag agttgatttc tgattgggct caagctagtg tcctgccttt
     3721 aaatttaaga gaacgtttga atcaagattg ccccttggat attacggccg aaatttcagt
     3781 tgatcccggc gataagtcta gtttaaaagc tttattgact tgggctgacg gcgtttccgt
     3841 ggaaacagtt ttaatgcgac atcgtgacgg tcgccacacg atttgtctat cttcacaggc
     3901 gggctgtcct ctgggttgtg ctttttgcgc taccggccaa ggaggttttt ttagaaattt
     3961 aagcgccgat gagattgtcc agcaatttat tttttgggcg cgctttttaa aacaggaaaa
     4021 taaaggcgaa agtataaata atgtggtgtt catgggtatg ggcgaacctt ttttaaatta
     4081 tgagcaattt attaaagcgg tgaaaatttt aaatgatcca gaaaaattta accttggttc
     4141 ccgccggatg tcggtttcta ccgtcggcat cattgaaggt attaaaaaat tatcgacgga
     4201 gaaattacag gttaatttgg cgatttctct gcacgccgcc gttgattctt tgcgtgaaca
     4261 gctgatgcct attgccaaaa aatattcgat tactagtatt ttaaaggcag tagacgagta
     4321 tatccgtaag actgggcggc gggtgatgtt cgagtatatt atgattaaga acgtgaatga
     4381 tggcgaagac gatgcgctgg ctctggcgca attaatgaaa aagcctcttt atttggttaa
     4441 tcttattcct tacaatccga ccggtaaatt tcaggcttcg tctcccgcca gaattaaaga
     4501 atttaagaaa aagcttgaat ctttaggcgt ggctgttacc gagcgtcaca gccaagggcg
     4561 gggaattagc gccgcttgcg gccagctagc cgggaagaaa aaatagattt gactaaaaaa
     4621 taagcgcctg cttgttcggt tagaaaggcg ctttttgaat ttcctcttta aaatgtcatt
     4681 aagtccggtt gttttttatc actatctatg ttaacaccga tattgaatag cccggcatct
     4741 tttgaggatt tttaaggttt ttaatggtaa ttaaccattt ttattatttt gttatattat
     4801 ttaatgcgat gaattagccc cttttatgct tttttgctat aataaaatca ctaataaatt
     4861 aatttttaat catatgttta ctctaaaatc tttgccttat gcttataatg ctttggcgcc
     4921 gcatatttct gaacagacta tgcgtttgca tcatgacaag catcatcaga cttatgttga
     4981 taatttaaat aagctgacag agggcacctc tttggcggcc atgtctttag ttgatgttat
     5041 taaagtcagc gctggtcagg cgaataaagc cggtattttt aataatgccg gccaggtcta
     5101 taatcatgat tttttttggg attgtttacg tccggctgat caagtgatgg ctattcccgc
     5161 cgctttgctg gaaaaaataa atcatgattt tggttctttg gagcaaatga tggttgaatt
     5221 taagacaatt gccttaagtc aattcggtag tggctgggct tggctggtgt tggctggaga
     5281 atctttacaa attattaaga cagctaatgc tgacaccgct tttctgcatg atgttaaccc
     5341 tttatttact ctagatgttt gggagcattc ttattattta gattatcaaa atcgtcgtgg
     5401 cgattttatt gatgcagtct taaataatct ggtgaattgg gactttgttg ccaatcaggc
     5461 tggtttataa atatgatgaa gaaaaatcct tacgcccgtc tccagcccaa gactcgagag
     5521 ccctatactg taatttatct agtgcgccac gctaaccctg attatcgtct agaaaagaaa
     5581 ttgggcgaca gactaatgcc tttatcaaaa gagggcaaac ttcaagcttc ttttttggct
     5641 aagcgtttac tgaaattaga tttagataca gtctatgcga gttcgttggc tagggcacaa
     5701 gaaactgctt ccatttttgc caatcgtcgt aagaagcggc caattataat tgatgagcgc
     5761 ttggatgaga ttgattggaa gaattggcat cgtgtcaaat attttcgcac ctcagaggag
     5821 cgccgtaagc aatatttgcc caattatcac gtcttagata aacagcttga taagatgcaa
     5881 gcccggacga ggcgcttttt agctgaagtc ttggctgaac atcgtgggca gagagtggct
     5941 ttattttgtc atggcaatat tattaagaca tttttaaccg gtattatgaa tgctgatgtt
     6001 cttggttttt tatctttgga gatatatcag gcgtccatca gtaagttagt catcgacggt
     6061 gacggttatg taaaaatcgc ttatattaat gacatcaatc atttaccgaa tcctccgctt
     6121 gaagatattt ttatcacttt agtttcttaa aggcatgtat tttcttcggt tattaaaatt
     6181 tataaccatt cctttactct cggcattttt tctttattat aatgccttac ctgtaaaagc
     6241 ggagactttg gccagtcgtt tgtctggtaa aattcttttg caagtagagt cggctgggga
     6301 ggcttggtat gttaatccgc ttgatggccg gcgttattat ctcggtcgtc cagccgatgc
     6361 ttttgtcttg atgaagcgtt taggtttggg catctccgag cagaattatc aattatttaa
     6421 agagacggga gcgcataatt tgcggggtcg aatattgtta cgcgcgcaag cgtccggaga
     6481 ggcttattac gttaatcccg acgacagtcg tctatattat ctcggtcgtc cgaccgacgc
     6541 cttcaatttg atgcgccgtt tcggtctagg catcagcaat cagaatttaa atcaaattgt
     6601 cgtcgctgat ttatcttcag ctgttaatga tctcaaggct aataccggta catattcttg
     6661 gcagtaccaa aacacaaaat atcagttaag ctttaattta gattcagctt tataccaatc
     6721 ttacgctagt agccctaagg tctatacgta ttacgttggg caagaaccgg ctgatatcaa
     6781 ggaggctttt tacgctttat ttttaaaagt aaaaagcaac gataatcaga ctttgggcct
     6841 tttgaattct ttgcgcgcac aggccgctag cttgggttta aatagtgatc agaccgccgc
     6901 ttttatttta gcttttattc agtatatccc ctatgaccac gccaaacttg agtctggcga
     6961 caatactccc tactatcctt ttgagacttt gtatttacat aaaggagtgt gtgctgacaa
     7021 gacattttta gcggtgcttt ggttgcgttc attgggttat ggtgccgcta ttttggattt
     7081 tcctgagagt aatcattcgg cagctggcat cgcttgtccg gtagctgatt ctttggctgg
     7141 ttccggttat tgttatgtgg aaactactaa ttattttccg ataggcgtag tcccacccct
     7201 gattacgggc ggtcaggcac agaatgatac ctcttctttt gaggatttat ttaactctga
     7261 ccatctcggt tatttggaaa tcaaacaaaa gagtcaaggt aaaatttatc aaggggtggc
     7321 ggcggtaaag agagaagcaa gggcgataga gcaagccaaa acagatttgg atgcttccca
     7381 aaatttatta aatgctaata agttgagcct tgacgcggct tatcagtctt taaaacagca
     7441 ggaaagccag ttgttagcct atcaagccgc tggcaatatt agcgcctata atcaattggt
     7501 tccgatatat aatcaagccg tggctgctta tcgagaccag accgaatcct ataatcaaag
     7561 cgttgttagt tataatcaag ccgttaatgc ttttaatgat cgctatcggc aattttatca
     7621 gcagtaattt gttgattatt agtaaatatt attcttaaaa gaggcttgac accttgattt
     7681 ttttaggcta ttattagaac agttgaggtt ttcctctggg tatacctctt tttatattaa
     7741 aattattatt aattaaacgt cacgttcgca taatttttct tggtttatat caaacaaaag
     7801 atatagatta gcgggcggaa gccttgtttt tttaaaagca agggacaaag aaaatctatg
     7861 gtaaaattac caagtttaga agaaatgcta aaagcgggta tgcatttcgg tcatcgtact
     7921 catcgttggc acccgaagat gaaaccttat atttttacag ctaagaacgg tatttatgtg
     7981 attgatttag agaaaagccg gcagatgttg gaagaggctt taaattttat gtcgcagttg
     8041 gtgagggagg gtaagtctat tttatttgta ggtaccaaaa gccaggtgaa agcaccgatg
     8101 aagaaaatgg cggaaatgat tggtcaacct tatatcgtcg gcaaatggct tggcggctat
     8161 ctcactaatt ttgctgtcgt aaaaaaatca gttaagaaat accaagattt aattgaaaag
     8221 cgcgatgctg gaaaattaga gaagtataca aaaaaagagc gtttaaattt tgatagagaa
     8281 attaaacgtt tggctgagcg cgttggcggc ttaacttctt taaacaagat gcctgatgcc
     8341 atttttgtct gggatataaa agaagaagaa acggcggtag cggaagcccg caaacgtcgt
     8401 atccccgtga ttgctatctg tgataccaac gttaatccag aattagttaa ttatccgatt
     8461 ccggccaacg atgatgccac taaaaccgtc aagttgattt tagaagtgat tcaatcagcc
     8521 attattgagg cgaagaagga tttaaaaaat taaattgatc cgccggacag gaggaatctg
     8581 tttcctgcgg ttttaaataa ttatatggaa attattaaac aattaagaga gatgactggc
     8641 gcgggcatag ttgattgcaa aaaagcctta gatgaggcta ataatgattt acgggtggcg
     8701 gtggagattt tgcgcaagaa gggaattgct aaagccgcta agcgtattga tcgcgaaact
     8761 aacgaaggcg tggttaaggt agcggttgcg gccgctgctg gcgaggctta tatctttgaa
     8821 cttacttctg aaactgattt tgtttcccgt aatgataaat tccagaaatt tgccgatgag
     8881 gtcatggact tagttttaag tttgcgcccg gaaaatttag aggcgctctt gtccttgccc
     8941 atggcagaaa gtaatgttaa ggataatttg gctgtattaa gcggtactat tggcgaaaag
     9001 atggaaatta gacgctttgc ccgtcttcac ggcgctagtt tagccgctta ttctcaccta
     9061 gggggtaagc tgggggtttt agtggcgctt gaccaagctg gtaaggaaga attggccatg
     9121 acggtggcga tgcaggtggc ggccgccaat cctaaatata tagaaccctc agaagtggag
     9181 gctggggaat tagaaaaaga aaaagaaatt tatcgtgaac agttacttaa ggaaggtaag
     9241 ccggaggcca tgattgaaaa aatattagaa ggaaaagtaa ctaagtatta tagtgaagtt
     9301 tgtttggttg aacaggaatt tattaaggac gacaagaaga aagttaaaga tatcctgggc
     9361 ggtgccaaga ttctgggttt tgtccgtttc agtctgtaat tgatattttt atttagtatt
     9421 ttaaagcccc gcgattatta attctattgg cgagtctttg ctttttatgc agattgccca
     9481 agtacaattt tctccatggg ataaggctta cagtttttat gttgattcta gtttaggtgt
     9541 tcttgtcggt gattttgtaa ttgtcgatac ggaattaggg cgtgagttgg gcaaaattat
     9601 ttctgtaagc aaggaatctt ctcccgagca ggatttaaag ctggttttac gtttagcgac
     9661 tgttgatgac gctcgtttag tttttgatga agaccgtcgt cgccaagcac ttgtcacttg
     9721 tcagggtttt attgagaggc atggcctgaa catgaaatta atcgatgttc gcttttcttt
     9781 ttccggtaat cgtatgactt ttgctttcgt ggccgagggt aggatagatt ttcgtgattt
     9841 ggttaaagat ttaaatgcag cttttaatgc aaatgtccgt ttattgcaaa ttggctcccg
     9901 tgatgaggct cgttactgtg gtgattgcgg tccttgcgga ttaagcttat gttgccggac
     9961 tttcgtccat aattttgcct cgattacttc agaaatggcg gaggctcagc aggtagtcca
    10021 ccgcggtagt gatcgtcttt ccggtatgtg tgggcgtttg atgtgttgcc ttagtttcga
    10081 atatgaaggt tataagcagt tggcaaagga attgccgcca ctgggaacaa agataagggt
    10141 gcaggggcgc acgggtatag ttgttgggca tcatctttta aaaaagaccg ttgatgtcca
    10201 attcgaagcg gaaaatgaag gggagagagc ttttgttatt caggttgacc caaatcagaa
    10261 caaagaaaaa gaattaaagc ctcgcattgg tcatttcgct aaatttcgcg ccaacggtaa
    10321 tactcgaccg agaccccgcc gttaatcatt ttttaaagaa gaagctcttg tccagggggc
    10381 tttttttgtg cccgaaatgt ggtataatgg gattagcttt tatttattta atttcataaa
    10441 tatgccttta aatctcgcta ttaatggttt tggccgcatc ggcagagccg cttttaagat
    10501 tattctcgat aaatacccag agcttaaagt tgtggccatt aatgatttga ccgataatca
    10561 aactttagct cgtcttctgc ggtatgattc ttgctatggt ttatatggtc atcgcgtgag
    10621 cggtgatgat cagggtttac tggttgacgg ccagcgctat actgtgtttg ccgaaaaaga
    10681 tccagccgct cttccgtgga aaaaaatgca agttgatgtc gtgctcgaat gtaccggaca
    10741 tttttgcgat aaggaaggag ccggtatgca tttacaggca ggggctaaaa aagttattat
    10801 ttccgccccg gccaagaagg gtggggtaaa aactatcgtt ttaggtgtaa atgataaaga
    10861 tttacgtgct agtgaccaca ttatttccaa tgcctcttgt actactaatt gtttggcgcc
    10921 agccatgtcg gtgcttcaag agaaatttgg tgttttaaaa gctttaatga ccacggtcca
    10981 ctcatatacg gctgatcaaa atttggttga cgggccccat aaagatcctc gccgagctcg
    11041 tgccgccgcc cttaatatta tacccaccac caccggggct gctgtggctg tcaccgaaac
    11101 cttgccttct ttaaaagata aattcgatgg tttggcggtg cgtgtgccca caccggtcgg
    11161 ctctctttgt gatatcgtct gcgttttgaa gaaaaagacc gacgttacca ctgtcaacaa
    11221 agcattttta gcggctgcca aaggcaaact taaaggcatc ttagaagctt ctaatgagga
    11281 gatagtgtca actgatattg tgggaaacgc tcatagctcg attattgatt tgaaaaatac
    11341 taaagtaata gctggtgatt tactaaaggt ggtcgcttgg tatgataatg agtggggtta
    11401 tgctaatcgt ttagtggact tagcggtaaa attaaaaaaa tttgttaaat aaataataga
    11461 ttgatgccat tatttttggc cattatcttc atttttatga ctaacaagat cagctcaaaa
    11521 gtaaattcgc ccctattgcc agcgacgcgc ttgttgacag atgcctggaa tttgtatgcg
    11581 tacaagctag ttgattttat cgaaatgtat gttttaggtc ttttcggttt gttgccactt
    11641 ttgcttttag gtattttagc cttagtattt tttgcttgga tgcatctaaa ctcttggact
    11701 atttatcttt tgtttggagt tttaggccta gccgctttgg cttgggcaat atattacggg
    11761 actcgggcaa agattggttt attgttaata ttgaagaatg agaaagctaa ggtcagagag
    11821 aactttcagg ctagtaaaag tttatttttc ccctatttct ttatcagttt agcgaccgga
    11881 gttttgattt ttttagcttt tttgcttttt atcattcccg gaataatttt atcagtattc
    11941 tggtctttgg ttgtaatatt gattgttttc gaagataagc tcacatttaa aacggcgact
    12001 aagagaagct gcgaactaat taaaggttat tggtggcccg tttttggccg ttttctcatt
    12061 attggagttt tagcaacaat agttgcttta atcatgaata ttcctatgaa ctatttatcg
    12121 gaaatcggca agcaggccta ctctttcgtg gttaatattt tttgggcatt acttagtccc
    12181 ttatttgtta cttacactta tttgttgtat aaagatttgc tttctaagaa atagctgatt
    12241 tttatccctt aattacgcat gatgattcct gattatattt tagaaataat ggagaggctg
    12301 atgtcggccg gttttagcgt agagatagtc ggcggtgccg tgcgtaatct tttaatgaat
    12361 aaacagccaa aagattggga tttaactact caagctaagc cgccagaaat attatcacta
    12421 tttcccgaag caaagtatga gaatgatttc ggcaccgtca ttattccttg tcgtactagt
    12481 gatggttctt tatctgacgt agtagaagtt accacctatc gtagtgaaca ggcttattct
    12541 gatcggcgcc atccggatga gattaaattt gaaaattctt tggaaaaaga tttagaaaga
    12601 cgcgatttta cgattaacgc tatggcttta attttggata aagccggcga atatgagtgg
    12661 agcggtcggc attttcgttt agttgatttg tttggtggct taaaggatat tcataaaaaa
    12721 gtgataagag ccgtgggcga gccatctgac cgttttaagg aagatgcctt aaggatgatg
    12781 agagcgattc gtttttctgc tcaattaggt ttttctttag aaccaaagac tcagcgtggg
    12841 gtcgttaaat tagccggtag tcttaagttt gtagccaaag agcgcattcg cgacgaatta
    12901 atcaagatta tcgcttctaa tcagccggcg gaaggtatcg cggctttaca tgaggctaaa
    12961 ctcttgcaat atatcatccc tgaattggaa cagggagtgg gtgtgcgcca aaatcatcac
    13021 cacatctacc cagttttcaa acataatttg ctgtcattaa aatattgtcc cagtcaggaa
    13081 tggaccgtac gtttagccgc tctttttcat gatatcgcca aaccaaaaac taggcgctta
    13141 attaacggtc aggttacttt ttataatcat gaatatgttg gtgcaaaaat ggtagatagg
    13201 ataatgacgc gcttgaagtt caagaatgaa gaccgagaaa aagtggtgaa cctggttaaa
    13261 aatcatatgt tctattataa cgtcggtgag gtaagcgccg cttccgttag acgtttaatt
    13321 agtaaggtgg gaagggaaaa tttacaggac ttaatttatt tgcgggttgc cgatagactt
    13381 ggctctggta cgccaaaggc tatgccctat aatttaaggc acttacagta tatgctggaa
    13441 aaggttcaga atgatccagt ctccgtcaaa atgctcgcta tcaatggcga tgatttaata
    13501 tcagtgttaa aactgacgcc tggaccgaaa tttggagcta ttcttgatgt gcttttaggg
    13561 gaagttttgg aagacccagc tcgtaatgaa cgttcctatc ttttagcgcg ggcggagaaa
    13621 ataaaggagc agaatcttga cgaaattcgc gagcaagctc gtaaattgat tgaggaaaag
    13681 cgtgatgacg acgaccagtc tcttcagcgg cgctttaaag tttgattttt attaattcgc
    13741 ttttaaaagc gttaaaatat aattttatga aaacatttta taaattgtt
//
LOCUS       DAOQSA010000022        13739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_3532_length_13739_cov_7.577097, whole genome shotgun sequence.
ACCESSION   DAOQSA010000022 DAOQSA010000000
VERSION     DAOQSA010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13739
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3532_length_13739_cov_7.577097"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(293..1057)
                     /locus_tag="PKK65_03045"
     CDS             complement(293..1057)
                     /locus_tag="PKK65_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020004954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81687.1"
                     /translation="MAKNASWEQIFKDYKILEHDFDKSPFDLSAEQIKKSCQKFKKTT
                     EKEVRILCKQDSRKSRPNIFVLNNLFILPVKNGFYNIIKGEGYVDIPEISSGIIEYET
                     QLGFSLDSSKVGNSEMQHLDFAYASSLIRTFMDDKSLVLTIRGRKYTPEFEFYVKKQK
                     IKVSSVQTEVDAGYEGKEQIVLIEAKNKKTKDTIIRQLYYPFRQWQSTTKKKVVTLFF
                     EKDDKTGVYSIWQFIFRNKNDYNSIKLVKSARYVIK"
     gene            complement(1064..1843)
                     /locus_tag="PKK65_03050"
     CDS             complement(1064..1843)
                     /locus_tag="PKK65_03050"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNU81688.1"
                     /translation="MCLYKKQKNFALYHGDCLEELKRFPENYFDMIFADPPYFLSNGS
                     FSCQNGRMVSVKKGEWDLSNGFQKDFNFHLEWIKECRRVLKPQGTIWISGTYHSIYQC
                     GFILQNTKYHILNDISWFKPNAAPNLSCRFFTASHETLIWARKEKKTKHTFNYQEMKN
                     GNWTEDEFKKPYLQMRSVWSVGTPKKEEKQFGKHPTQKPLDLLKRIILASTKEGDIVL
                     DPFTGSSTTGVAANLYKRNFVGIDSDKNYLNLSLQRLNALV"
     gene            complement(1847..2683)
                     /locus_tag="PKK65_03055"
     CDS             complement(1847..2683)
                     /locus_tag="PKK65_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HNU81689.1"
                     /translation="MNLNIQKKRVEPFLKWAGGKSQLLEQFKSLFPKEGSYRRYIEPF
                     IGGGAVFFYLEPKEAIISDLNKDLIEAYKIVKSHPKELISLLKKYEKQHSKEFYLKIR
                     DEYNANKLDKINKAAHLIYLNKTCFNGLYRVNSKGEFNVPFGQHKKFTVNKEGILAAS
                     KILKKTKIEHTDFTQVLKNAKKGDFVYFDPPYYPLNTGSNFTTYTRENFLEKEQEKLK
                     ETFKKLDARGCILMLSNSDTPYIRKLYKEWNITKVSAKRFINCIAEKRGDVFEVVVRN
                     YK"
     gene            complement(2690..3976)
                     /locus_tag="PKK65_03060"
     CDS             complement(2690..3976)
                     /locus_tag="PKK65_03060"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNU81690.1"
                     /translation="MFKSTVLKQKQEKERLLSLPYIERTKEKEAKKWLNSDLIKVVLG
                     PRRAGKSVFSLMLLKDQSFAYFNFDDESLPGEEKFDLDELMSELRQAYGDTKYVLFDE
                     IQNLPKWELFVNRLHREGYNLVLTGSNARLLSKELATALTGRHIPIQILPFNFTEFLR
                     AKGYELSVDKLSLPEEKAKFLGYLNEYMINGGYPEVITKNLDPRGYLDVLFDSLLFKD
                     VVKRHKIRFSEQIDDLGSYLINNVAGQYSIRKLANILGFKSGITLERYLGYLTEAYLI
                     FFLHRHSAKVGLRLKSPKKAYVVDNGFITAKTVQPSPNTGKLMENLVFTELVKRSNES
                     NREIFYYRTRNDREIDFAVKKGTEIVELIQVCYEANNQDVKQREIKALIEAGKELNVT
                     KLSVLTWNDKRTVEKDGFTIQFKPLWEWLIEGVQTA"
     gene            4132..4554
                     /gene="mscL"
                     /locus_tag="PKK65_03065"
     CDS             4132..4554
                     /gene="mscL"
                     /locus_tag="PKK65_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010286030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNU81691.1"
                     /translation="MKLLKEFKEFAIKGNVIDLATAVIIGGAFGKIVSSLVADIIMPI
                     VGLLTGGTDFSAWKIVLRSATAERAALNLNAGIFIQNIVDFLIISASVFLMIKILLQI
                     KNKFKKEEEKKEILPTPPEPSAESKLLAEIRDILKNKE"
     gene            4596..>4898
                     /locus_tag="PKK65_03070"
     CDS             4596..>4898
                     /locus_tag="PKK65_03070"
                     /inference="COORDINATES: protein motif:HMM:NF024784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNU81692.1"
                     /translation="MAVNEEATYENKNGYLCYKSNGKLVHRCVMEEYLAQRGEKLYPE
                     EVVHHIDGNIQNNDIDNLEVFENQSEHAKHHLEERKEEENDDDYDDDDYDDDDYDDD"
     assembly_gap    4899..4998
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5181..5423)
                     /locus_tag="PKK65_03075"
     CDS             complement(5181..5423)
                     /locus_tag="PKK65_03075"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNU81693.1"
                     /translation="MRAFGIEIYVVLDKKEIVGTLRLLLALKFYYQGRLAVHIEDAAT
                     RVDYQGKGVASALMRRVLEICRGKNCYRIILIFVFI"
     gene            complement(5556..6899)
                     /locus_tag="PKK65_03080"
     CDS             complement(5556..6899)
                     /locus_tag="PKK65_03080"
                     /inference="COORDINATES: protein motif:HMM:NF022114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HNU81694.1"
                     /translation="MKQLGLRRFLFLLGLFLIVVGFYLAVREWLIRRPSLAIPSTEVV
                     FQPSSVTYFIDGQVITLMNGNSADNKVSIFGQPVFGDLNNDGRDDAAVLLLEESEGSG
                     SFFYVAAAINLNSGIQGTNAVFLGDRIALQNLEIRGGQIIVNYVDHQEGEPMTTSPSV
                     DVSAYFFIQDGILQKIEPSAKINSYLISDEDASKYCNGAKMDSDGYRQTITTEKPLLL
                     ADGNYDLKQIATIVIKAATSGNCQQIASNLGISSDGATVYIAPVEAWAGISITMCSCQ
                     PQVEVNLLRLPGIKRVRWLSAVDNFEECSALSGVVMESYPRQCRYGGQNFTENIGNEL
                     EASDNIILESPRPNQDIISPLTVKGRARGSWFFEGSFPVFLTDWDGKIIAQGVAQAQG
                     DWMTNDFVPFEVTLSFVADKDAYSNRGSLILKKDNPSGLAGLDDALEIPVMIATD"
     gene            complement(6911..7615)
                     /locus_tag="PKK65_03085"
     CDS             complement(6911..7615)
                     /locus_tag="PKK65_03085"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNU81695.1"
                     /translation="MTLDITTHKNILIRILKDIFTDSQVGPFLGFKGGTAAFLFYNLN
                     RFSVDLDFDLLDATKEDQVFNRVKGILESYGTLKEAKMKKFNFFYLLSYEEKVIGAQN
                     MKVEINRRDFGSQYEVKSYIGIPMKVMIKEDMAAHKLCAMYERIGKTNRDIFDVYFFL
                     KDNWPVNKQIVEKRTGMTYKEFLQKNIELLAKTTDRNFLSGMGELLDEKQKAWVKSKL
                     RTETIFLMKLALDNEK"
     gene            complement(7620..8207)
                     /locus_tag="PKK65_03090"
     CDS             complement(7620..8207)
                     /locus_tag="PKK65_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81696.1"
                     /translation="MGKANILNILKSNNTVFSFKEILLASGESNPALLRRRIYSYIKN
                     GQLYSIRRGLYAKDKNYNKLELATKIFIPAYVSFETVLAEAGVTFQHYSQIFVASYQT
                     KEIDCDGQIFSFKKLKTNVLNNNSGIENKGFYSVASKERAFLDIVYLNKDYHFDNLSP
                     LAWDKVFSILPMYGNKRMAQKVKQYYKDFKSKQSQ"
     gene            complement(8316..9767)
                     /gene="cysS"
                     /locus_tag="PKK65_03095"
     CDS             complement(8316..9767)
                     /gene="cysS"
                     /locus_tag="PKK65_03095"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNU81697.1"
                     /translation="MKIYNYKTKKKENLITIKEGEIKMYVCGITPYDSPHIGHVLVAL
                     RFNIIRNYLTYKGYKVFFVQNITDIDDKIINKAKQKRVSHNTISSLYIKEYEQILTAF
                     NIKKPDYNPRVTKYIDKIIIYIQDLIAKGYAYTTEKGNVYFDVSKKLDYGKLSGQELE
                     KLQGKVWEKEQDKKNVLDFALWKSENEAGFYWLSPWGKGRPGWHIECSVMSNDILGES
                     IDIHGGGLDLNFPHHENELAQCEAHNSKPFVNYWMYSGLLNINGVKMSKSLGNFITAK
                     DGLDKYGAELLIYVINTFHYRSDINLQDRIFIDNLNNIANFHKTFILVEDNYNTNINL
                     DNFELEEISIIINDFFVAMDDDFNTAKALVVLNKILLILINKIRNRANEKEIVYLYQK
                     IKNLGAILNLFFNKNSEDVLNELFKFYSEYLYKPKLTLEKINIEIEKIKNALEKKNYF
                     LSDRIRGNLLDRGIKVMQMENKNIEWQFAFVEK"
     gene            complement(10042..10905)
                     /locus_tag="PKK65_03100"
     CDS             complement(10042..10905)
                     /locus_tag="PKK65_03100"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNU81698.1"
                     /translation="MARYYKRRYKNYKEDDGVKFISGLIVFFIVCLILAYNAGTATFF
                     ATLASEVGLIVLVIVGSEVLKKSKENARKAKVENILNTIQKAGLEEYINNFISRFGLG
                     QEKNKNVWTRRNYKISWDRINDLKDFLYQKGIDFSFSEICILLANYIDKKEFDLTVNS
                     ISATTNSFSKLSGSDFEHLLYRLYETMGYSVLFNGKTGDQGGDLIATKNQERILIQAK
                     CYKDWSVGNSAIQEAVAARNHYDCNKAMVITTSVFTKEAMELAKTNKVELIPKELLQK
                     MLLDNLKESWS"
     gene            complement(10914..13607)
                     /locus_tag="PKK65_03105"
     CDS             complement(10914..13607)
                     /locus_tag="PKK65_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNU81699.1"
                     /translation="MNNLWHTKSSTAVIEELHSSERGLNSQEVTLRLKQYGLNKLPDA
                     KVDGIFTIFLRQFQSPLIYILVAASIIVFAMGEVVDGSIILAILLFNAIVGTIQEGKA
                     QNTLLALKKFVETKATVLRDGKEIIISDTELVPGDILILQEGERIPADSRVILSHNLK
                     VDEAALTGESEPIHKTSERQAAGETVGQYKNIVYKGTNIVAGNGRALVIATGSQTAIG
                     TIASKIASVNTEIPLKGNIRYLSRFIIAIVAIISVVIFILGIAVGKPVKDMFTTIVSL
                     SVSIIPEGLPIVVTLILAMGVWRMSKRNALVKKLQAVEALGQASIIAVDKTGTITKNE
                     LVIQKVYVAEKMFDVEGIGYEPKGKISLDGRIIDAANYPELLLAGKIGTFCANARIMY
                     GKEDKRWRVSGDPTEAAMLVFSQKLGFHKEDIERESPLVGESPFDYRLKYHAVLHNIE
                     NKNFLTVVGAPEVILKLSETIWLDGKRKELTPAERKNLESIFYQMSQEGLRVLAFAIN
                     PDAAGKILKPEKIRGLSFVGFYGMRDATRPEAASAIKKAESAGVKVVMITGDHKITAQ
                     AIAKEIGIWHEGDEVLTGSEIDGMSDAELSKRLKSISVFARVNPEHKMRIISAYRARG
                     EVVAMTGDGVNDAPSLVAADLGVAMGNIGTEVAKEASDIVLLDDNLESIVSAIEEGRN
                     IYKTIKKVILYLFSTGMGEVFAIIGSLILGYPLPILAAQIIWLNFVTDGFIDVSLAME
                     PKEHHLLDDKFEKPNKFLVDKLMAKRMVLMSLVMAFGSLILFKDYYEGDLTKARTMTL
                     TVLAVFQWFNAWNCRSESKSIFRMNPFSNKYLVGATIIVIVLQLLVVYTPVMNTFLKT
                     APLNLSDWLLIVPIATSIVLIEEIRKFFYRRRLAKKTLKSN"
BASE COUNT         4272 a         2736 c         2100 g         4531 t
ORIGIN      
        1 accaaataga cttgaaaccg gaggccaaag ttacattctt ggattataaa tcgaattagc
       61 tcatggttac atttttagac tatttgacac gttaaggttt ttttaccaat taaataagtt
      121 atttaaagcg atttcaatct tttttgtatt ggtaaaaaca tttttcttaa tttacaaaat
      181 acaaccaaag catattgctt ggtttaagat attttataaa ataattaaaa acttgatcgt
      241 cgttttttac gtcccaatat atctttatga aatttaaaaa gcttcatata aattatttaa
      301 ttacatatct ggcagatttt accaatttaa tgctgttata atcattttta tttctgaaaa
      361 taaactgcca aattgaataa actccggtct tatcgtcttt ttcaaaaaac aaagtcacaa
      421 cttttttctt tgttgttgat tgccattgtc taaatggata atacaattgt cttataatag
      481 tatcttttgt ttttttattt tttgcttcta ttaaaacaat ctgttcttta ccttcataac
      541 ccgcgtcaac ttcggtttgg acacttgata cttttatttt ttgcttttta acataaaact
      601 caaattctgg cgtatatttt ctccccctta tagttaagac taaagattta tcatccataa
      661 aagttctaat caaactagat gcataagcaa aatccaaatg ctgcatctcc gaattaccaa
      721 cttttgatga gtctaaagaa aaacctaact gagtttcgta ttcaataata cctgatgaaa
      781 tttctggaat atcaacatac ccttctcctt ttattatatt ataaaaacca ttttttaccg
      841 gcaaaataaa taaattattt aaaacaaaaa tatttggacg actttttcta ctgtcttgtt
      901 tgcataaaat ccgaacttct ttttcggtag tttttttaaa tttctgacaa gattttttta
      961 tctgttcggc tgacaaatca aacggtgatt tatcaaaatc atgttctaaa attttataat
     1021 ccttaaatat ttgttcccaa gatgcgtttt tagccatata tttttatact aatgcattta
     1081 atctttgcaa agaaagattt aaataatttt tatcggaatc tattccaaca aaatttcttt
     1141 tatataaatt agcagccact cctgtggttg agctgccagt aaatggatca agaactatat
     1201 cgccttcctt agtgcttgct agtattattc tttttaataa atctaatggc ttttgcgttg
     1261 gatgtttacc aaattgtttc tcttcttttt taggtgtgcc cacagaccaa acagagcgca
     1321 tttgtaaata tggtttttta aattcatctt cggtccaatt accatttttc atctcttgat
     1381 aattaaaagt gtgttttgtc ttcttctctt ttctagccca aatcaaagtt tcatgactag
     1441 cagtaaaaaa tcgacaactt aaattagggg ccgcatttgg cttaaaccat gatatatcat
     1501 ttagaatatg atatttggtg ttttgcaaaa taaaaccgca ctgataaata gagtgataag
     1561 ttccgctaat ccaaatcgtt ccctgaggtt taagtactcg acgacactcc ttaatccatt
     1621 ctaggtgaaa attaaaatct ttttgaaaac cattgcttaa atcccattca ccttttttaa
     1681 cactaaccat tcttccattt tgacaactaa aactaccgtt tgacaaaaag tatggcgggt
     1741 cagcaaaaat catatcaaag taattctcag ggaatctttt taattcctcc aaacaatcac
     1801 catgataaag agcaaagttt ttttgctttt tatataaaca catatattac ttataatttc
     1861 ttactacaac ttcaaataca tctcctcttt tttcggctat acaattaatg aaacgcttag
     1921 cgcttacttt agtgatattc cactctttgt ataactttct aatataaggg gtatctgaat
     1981 tgcttaacat aagtatacac ccccgggcat caagtttctt aaaagtttct tttaattttt
     2041 cctgttcttt ttctaaaaag ttttccctgg tataagtagt aaaatttgag ccagtattta
     2101 atggatagta tggaggatca aaataaacaa agtctccttt cttggcattc tttagtacct
     2161 gtgtaaaatc agtgtgttct attttagttt tttttaatat tttactggca gctaatatgc
     2221 cttctttatt aactgtaaat ttcttatgtt gtccaaatgg aacattaaat tcccccttac
     2281 tattaaccct ataaagacca ttaaagcagg ttttatttag ataaattaga tgagctgctt
     2341 tatttatttt atctagttta ttagcgttgt attcatctct gatttttaaa taaaattctt
     2401 tactgtgttg tttttcgtat ttttttaaca aagatattaa ttctttggga tgacttttta
     2461 cgattttata agcttcaatt aagtctttat ttaaatcaga aataatagct tcttttggtt
     2521 ctaaataaaa aaagacagca ccaccgccaa taaatggttc aatgtatcgt ctataacttc
     2581 cttctttggg aaataatgat ttaaattgtt ccaataattg cgatttacca ccagcccatt
     2641 taagaaacgg ctcaactctt tttttctgta tatttaaatt cataaataat taagcagttt
     2701 gaaccccctc aataagccat tcccacaatg gcttaaactg tattgtgaaa ccgtcctttt
     2761 ctactgtgcg tttatcattc caagtaagca ctgagagttt tgtcacgttc aattcttttc
     2821 ctgcttctat taaagctttt atttctctct gtttgacatc ctgattattt gcttcgtagc
     2881 aaacctgtat taattccact atttctgttc ctttcttcac ggcaaaatct atttcccgat
     2941 catttctggt tcggtagtag aatatttctc ggttagattc gttgcttcgt tttactaatt
     3001 cggtaaagac aagattctcc ataagttttc ccgtgttcgg tgaaggctgt actgttttgg
     3061 cagttataaa accgttatct acgacatatg ccttcttggg agactttagt cgtaatccga
     3121 ctttggctga atggcgatgt aaaaaaaata taagataagc ctcagtaaga taaccgagat
     3181 atctttcaag agttattccg cttttaaaac cgagaatatt ggcgagcttg cgaatactat
     3241 actgaccagc gacattattt ataagatatg agccaagatc atctatttgc tcggaaaatc
     3301 ttatcttgtg acgtttaaca acatccttaa aaagtaagga gtcaaataac acatcaagat
     3361 atcctcgcgg atcaagattc ttggttatca cttccggata cccaccattt atcatgtact
     3421 catttaaata tccaagaaat tttgcttttt cttctggtag tgaaagttta tcgaccgaca
     3481 attcatatcc ttttgctcgc aaaaattcag taaaattaaa tggcaatatt tgtatgggaa
     3541 tatgtctacc agtgagggcc gtggccagct cttttgaaag caatctcgca ttagaaccgg
     3601 taagtactaa attatatcct tctcgatgca aacgattcac aaatagctcc catttgggaa
     3661 ggttttgtat ttcatcaaaa agaacgtatt ttgtatctcc gtaagcttgt cgtaattcag
     3721 acataagttc gtcaagatca aacttttctt caccagggag agattcgtca tcaaagttaa
     3781 aataggcgaa agattgatcc tttaaaagca taagagaaaa gactgatttg ccagctcgac
     3841 gaggccctag aacaactttt attaaatcag aattaagcca ctttttggcc tctttttctt
     3901 tagtcctctc tatataaggc aaagagagaa gcctctcttt ttcctgtttt tgctttaaaa
     3961 ctgttgattt gaacatatta tttatgctat attatacata aagttccttt tttgcacact
     4021 acaagtaagt ataatataca aaataggaaa atacaagagt ttcgcaatgc taaaaaaaca
     4081 aaacttggat aaagaacaat tttatgctaa aatacaacta aattaaaatt catgaaatta
     4141 ctaaaagaat tcaaagaatt tgccattaag ggcaatgtta ttgatttggc caccgccgtt
     4201 atcattggtg gcgcttttgg caaaattgtc tcctccctag tggctgatat tattatgcct
     4261 atagtcgggc tgttaacagg cggcaccgat ttcagcgcct ggaaaattgt cctccgttct
     4321 gccacagccg aaagggcggc tctaaacctt aatgccggta tttttatcca aaacattgta
     4381 gatttcctta taatctccgc ctcggtcttc ctgatgatta aaatacttct acagattaaa
     4441 aataaattca agaaagaaga agaaaagaaa gaaatattgc cgacgccccc tgagcccagt
     4501 gctgaaagca aacttctcgc tgaaatcaga gatattttga aaaataaaga ataattataa
     4561 aagaaagtta cttattattg caacaatata aaaatatggc agttaatgaa gaggctactt
     4621 acgaaaataa aaatggttac ctttgttata aaagtaacgg caaacttgtt catcgttgtg
     4681 taatggaaga atatttagct caaagaggtg aaaaattata tccagaagaa gttgttcatc
     4741 atatagacgg gaacatacaa aataatgata ttgacaattt agaagttttt gaaaatcaaa
     4801 gtgaacacgc gaaacaccac cttgaagaaa gaaaagaaga agaaaatgat gatgactacg
     4861 atgatgatga ctacgatgat gatgactacg atgatgatnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnat gatgatgact acgatgatga tgactacgat gatgatgact
     5041 acgatgatga tttttaatct taatctataa caacatcctc ttaacactca aaaacccgct
     5101 taaatgagcg ggctttttat aacaataaac taatggataa caataaagtc gacaattaat
     5161 cgtgaaaatc taaacacaaa ctatatgaag acgaaaatca aaatgatgcg ataacaattc
     5221 tttcccctgc aaatttctaa aacccgccgc attaaagcgc tagctacgcc cttcccttga
     5281 taatcgacgc gagtagccgc gtcttcgata tgaacagcca aacgtccttg ataatagaat
     5341 ttaagagcta acaacaagcg caaagtaccg acaatttctt tcttgtctaa aacaacgtaa
     5401 atttcaatac caaacgctcg caatcttgaa atatcttttc agccatctct acacttaaat
     5461 tcttaaaagg ccgcaaattg gttagggttt aaaaaaagaa ggacagctta aatcttctaa
     5521 actaagtttc tggtataata agacctacac caaacttaat cagtcgcaat cattactggt
     5581 atctccaaag cgtcatcaag accggctaaa ccggaaggat tgtctttctt taaaattaaa
     5641 ctgcctcgat tgctataagc gtccttatcg gccacaaaag ataaggtgac ttcaaaggga
     5701 acaaaatcgt tcgtcatcca atcaccttgt gcttgagcca caccctgagc aataatctta
     5761 ccatcccaat cagtcaaaaa gaccggaaaa ctgccttcga aaaaccaaga accgcgcgcc
     5821 ctgcccttta ccgttaaagg actaatgata tcttgattgg ggcgaggaga ctctaaaata
     5881 atattatcag acgcctctaa ttcattgccg atattttcag taaaattctg tccgccataa
     5941 cggcattggc ggggatatga ttccatgaca acgccgctta aagcggagca ctcctcaaaa
     6001 ttgtcgaccg ccgacaacca ccttactctc tttatccctg gtaaacgcaa aagattaact
     6061 tcaacttgcg gctggcaact gcacatagta atggaaatac ccgcccaagc ttcaaccgga
     6121 gcgatataga ctgtagcgcc atcactagaa atgcccaaat tgctagctat ctgctggcaa
     6181 ttaccgctgg tagcggcctt aatcacaatg gtcgctattt gctttaaatc ataatttcca
     6241 tccgctaaaa gtaaaggttt ttccgtggta atcgtctgac ggtagccgtc gctatccatc
     6301 ttggcgccat tacaatattt ggaagcatct tcatctgaaa ttagataaga gttaatttta
     6361 gccgatggtt caattttttg taagatgccg tcttggataa aaaaataagc gcttacgtcg
     6421 actgaaggcg aagtagtcat cggctcgcct tcttgatggt caacataatt gacaattatc
     6481 tgaccgcccc taatttcaag attctgcaaa gcgattcgat cgccaaggaa aacagcattg
     6541 gtaccctgaa tgccagaatt aagattgatg gcggcggcga catagaaaaa agaaccgctt
     6601 ccttcagact cttcaagcag taaaaccgcc gcatcatcgc gaccgtcatt gtttaaatcg
     6661 ccaaacaccg gctgaccgaa tatgctaact ttattgtcag cggaattccc attcatcaga
     6721 gttatcactt ggccatcgat aaaataagtt acagacgatg gctgaaaaac gacctcagta
     6781 gaaggaatag ctaaagaagg ccggcgaata agccattccc tgacggccaa ataaaatccg
     6841 acaactatca aaaataatcc taacaaaaat aaaaatctcc ttaatcccaa ttgcttcata
     6901 tattatatct ttatttttca ttatctaacg ccagcttcat caggaatatt gtctctgttc
     6961 tgagtttcga tttaacccaa gccttctgtt tttcatctaa gagttcgccc ataccggaaa
     7021 gaaagttgcg atccgtagtc tttgccaaca actcaatatt cttctggaga aattctttat
     7081 aggtcatgcc ggtacgtttt tccacgattt gtttattaac tggccaatta tcctttaaaa
     7141 agaaatagac atcaaaaata tctcggttgg ttttaccaat tctttcatac atcgcacaaa
     7201 gtttatgagc cgccatatct tccttaatca tcactttcat tggaataccg atgtatgact
     7261 taacttcata ttgagaacca aaatctctgc gattaatttc cactttcata ttttgagccc
     7321 caataacttt ctcttcataa gaaagcaaat aaaaaaaatt aaactttttc attttagcct
     7381 ccttaagagt tccgtagctt tctaagatcc ctttaactct attgaaaact tgatcttctt
     7441 tagttgcgtc caacaaatca aaatcaaggt ctactgaaaa acgattgaga ttgtaaaaaa
     7501 gaaaagcggc cgttcctccc ttgaaaccaa gaaaaggtcc aacctgggaa tcggtaaata
     7561 tgtccttaag gattctaatg agaatatttt tatgtgtagt tatatctagt gtcataaatt
     7621 tattggcttt gcttagattt aaaatcttta tagtattgct tgactttttg ggccatgcgc
     7681 ttatttccat acatcggcaa aattgaaaat accttatccc aagctagagg cgacaaatta
     7741 tcaaaatggt aatctttgtt taaataaaca atgtctaaaa atgctctttc ttttgaagcc
     7801 accgaataaa atcctttgtt ttctattccc gagttattat taagaacatt ggtttttaat
     7861 ttcttgaaag aaaaaatctg tccatcgcaa tctatttctt ttgtctgata ggaagcaaca
     7921 aaaatttggc tgtaatgctg gaatgtaact ccggcttcag ctaagactgt ttcaaaactt
     7981 acataagccg ggataaatat ctttgttgct aattcaagtt tattataatt tttatctttg
     8041 gcatataacc ccctgcgaat actgtacaac tgtccatttt ttatatagga atatattctg
     8101 cgtctcaaaa gagccggatt actctctcca gaagctaaca gtatctcctt aaaagagaag
     8161 accgtattat tacttttgag aatattcaaa atgttagctt ttcccatata tttatacttt
     8221 atcagttaaa tgttacccgt gatgttacct ttaactctta ttatatacta caataaaaac
     8281 taatacaagg tggttaatag acataatatc ctatgttact tttctacaaa agcaaattgc
     8341 cattcaatat ttttattttc catttgcata acttttatgc ctctatctaa taaattccct
     8401 ctaatcctat ccgacaaaaa ataattcttt ttttctaaag catttttaat tttttctatt
     8461 tctatattta ttttttctaa tgttaatttg ggcttatata aatattcact gtaaaattta
     8521 aacaactcat ttaaaacatc ttcgctattt ttattaaaaa ataaatttaa aattgctcct
     8581 aaattcttaa ttttttgata caaatagact atctctttct cgttagcccg atttctaatt
     8641 ttatttatta aaattagaag aattttattt aaaacaacta atgctttcgc tgtattaaaa
     8701 tcatcatcca ttgctacaaa aaaatcattt attattatac ttatttcttc taattcaaaa
     8761 ttgtctagat ttatatttgt attataatta tcctcaacta aaataaaagt tttatgaaaa
     8821 ttagcaatat tgtttaaatt atctataaat atcctatctt gaagattaat atcggatcga
     8881 taatgaaaag tgttaattac ataaatcaat aattccgctc catatttatc tagtccgtct
     8941 ttagcggtga taaaattacc taaagattta gacattttta ccccatttat attcaataac
     9001 ccgctataca tccaataatt aacaaaaggc ttgctattat gagcttcgca ctgagctaac
     9061 tcattctcat gatgaggaaa attaagatct aacccccctc catgtatatc aatactctct
     9121 cctaagatat cgttactcat aacagagcat tctatatgcc aacccggtct tcccttaccc
     9181 cacggagaca accaataaaa acccgcttca ttttcagatt tccataaagc aaaatctaaa
     9241 acattttttt tatcctgctc tttttcccaa actttacctt gtaatttttc taattcctga
     9301 ccagacaatt taccataatc taattttttg ctcacatcaa agtaaacatt tcctttttct
     9361 gttgtataag cataaccctt ggctattaaa tcttgtatat aaataattat tttatcaata
     9421 tactttgtaa ccctgggatt ataatctggc tttttaatat taaaagcggt taaaatttgt
     9481 tcatattctt taatataaag ggaagaaatg gtattatggg aaactctttt ttgtttagct
     9541 ttatttatta ttttatcgtc aatgtcggta atattctgca caaaaaaaac cttatatccc
     9601 ttatatgtaa gataatttct gatgatattg aatcttaaag caactaaaac gtgtcctata
     9661 tgaggagagt cgtatggggt aataccacaa acatacattt taatttcacc ctctttaata
     9721 gtgattaaat tttctttctt ttttgtttta tagttatata ttttcattaa taacttaact
     9781 taacaatctt tttatctttt taatattgac gaaaatagca cttttaatat tataaccctc
     9841 gttttcgggc aattctttta catagtttaa ccatttacca tcagtataat ttttggcctt
     9901 gcctattaat tcaaaaccaa cacaagattt tttccattct ggattccaaa ttaccagtga
     9961 aacattatta ttataattta tatttttaac atatcttata aatctgctcc gaagttggta
    10021 ttattattat ttatttgccg cctagctcca actttctttt aaattatcta aaagcatttt
    10081 ttggagtaat tctttaggga ttaattccac tttatttgtt ttcgctaatt ccatggcctc
    10141 tttagtaaaa acgctcgtag tgataaccat ggccttatta caatcgtagt gatttctagc
    10201 ggcgacggcc tcttgaatgg cactattgcc cacgctccaa tctttataac attttgcctg
    10261 aatcaaaatt ctctcctgat ttttagtcgc tattaagtcg cccccctggt ctcctgtttt
    10321 tccattaaac agtacggaat accccatagt ctcataaaga cgatataaca aatgctcaaa
    10381 atcagaccca cttaattttg aaaaactatt agtggttgcg ctgatgctat taacggttag
    10441 atcaaactct ttcttatcaa tgtagttggc taaaaggata caaatttctg aaaaagaaaa
    10501 atcgattcct ttttgatata aaaaatcttt taagtcattt atcctgtccc aactgatttt
    10561 ataatttctt ctagtccaaa catttttatt cttttcctga ccaagaccaa atcgggatat
    10621 aaaattattg atatattctt caagtcctgc tttttgaata gtgtttaata tattttcaac
    10681 tttggcttta cgggcatttt ctttactctt ttttaaaact tcactcccaa caattaccaa
    10741 aacaattagc ccaacctcac tggctagggt agcgaagaaa gtggcggttc ccgcattata
    10801 agccaatatc agacaaacga taaaaaagac aataagtccg ctaatgaatt taacgccatc
    10861 gtcttccttg taatttttat atcttctctt gtaatatctg gccataaatt tgattaatta
    10921 ctttttagag tctttttcgc caatcgtctg cgataaaaga atttgcgaat ttcctcaatc
    10981 agcacgatcg atgtcgctat cggtacaatg agcaaccaat cggaaagatt aagtggtgcg
    11041 gttttcaaga atgtattcat caccggagtg taaacaacga gcagttgaag cacgatgaca
    11101 atgattgtcg cgcccactaa gtatttgtta gaaaacggat tcatcctaaa aatagattta
    11161 ctctcggatc tgcaattcca agcattaaac cactgaaaga cggccaaaac ggtaagtgtc
    11221 atcgtccggg cttttgtcaa atccccttcg tagtaatctt tgaaaagaat aagagaaccg
    11281 aaagccatga caagagacat aagcaccatt ctttttgcca ttaatttatc aacgagaaat
    11341 ttattcggct tctcaaactt atcgtcgagc aaatgatgct ctttcggttc catggccagc
    11401 gacacatcaa taaagccgtc agtgacgaaa ttcagccaga taatctgtgc cgctaatatt
    11461 ggcaaggggt atcccaaaat aagggatcct ataatcgcaa acacctcccc catgcccgtt
    11521 gagaaaagat ataagataac tttcttgatg gttttgtaga tattgcgtcc ctcctcgatt
    11581 gctgacacaa tactctccaa gttgtcgtcg agaaggacaa tatcagaggc ctccttcgcc
    11641 acttcggtgc cgatattacc catcgctact ccaagatcgg cggcgaccaa cgagggcgcg
    11701 tcgttaactc cgtctccggt catggccacc acttctccac gcgcccgata ggcgctaata
    11761 atcctcattt tgtgttccgg attcactcga gcaaaaaccg atatactctt gagtcgtttc
    11821 gacaattcgg cgtcggacat tccgtcaatt tccgacccgg taagcacttc gtcaccttca
    11881 tgccaaatac caatttcttt agctatagct tgggcagtta tcttgtgatc accagtaatc
    11941 attactacct tcactccggc agactcggct ttctttatgg ccgaggccgc ttccggtctg
    12001 gtcgcgtctc tcatgccata aaaaccaaca aaagaaaggc ctcgtatttt ttccggcttg
    12061 agtattttcc cggcggcgtc aggattaata gcaaaagcaa gcacgcgcaa gccttcttgc
    12121 gacatctgat aaaaaataga ctcaagattt tttcgttctg cgggtgtaag ttctttgcgt
    12181 ttgccgtcca accagattgt ctctgatagt ttcaagataa cttcgggggc accaacaacg
    12241 gtcaaaaaat tcttattttc aatattatgg agaacagcgt gatattttag cctgtaatcg
    12301 aacgggcttt ctccaacaag cggagactcg cgttcaatat cttctttatg aaagcctagt
    12361 ttttgagaaa acacgagcat ggccgcttcg gtaggatctc cggagacccg ccagcgtttg
    12421 tcttcttttc cgtacataat ccgggcgttg gcgcaaaaag taccaatctt tccggcaagt
    12481 aaaagttctg gataattggc ggcgtcaata atacgaccgt ccaagctaat cttccccttt
    12541 ggttcatacc caatgccttc gacatcaaac atcttctctg ccacatacac cttttgaatc
    12601 accagctcgt ttttggtgat cgtgccggtt ttatcaaccg caataatact tgcttgtccc
    12661 aaagcctcaa ctgcctgcag tttctttacg agcgcattac gcttgctcat gcgccaaaca
    12721 cccatagcga gaatgagagt gacgacaata gggagtcctt ccggtatgat tgataccgac
    12781 aaagaaacaa tggtcgtaaa catatcttta accggcttgc caaccgcaat gcctaaaata
    12841 aagatcacta ccgaaataat cgcgacaatg gcgatgataa agcgtgaaag gtaacggata
    12901 ttgcccttaa gcggaatctc ggtattaacc gatgcaatct tgctggcaat tgtaccaata
    12961 gcagtttgtg aaccagtggc aataactaac gctcggccat tgccagcgac aatattcgtc
    13021 cctttgtaca caatattttt atactgcccg accgtttctc ccgccgcctg cctttcactc
    13081 gttttatgta tcggctccga ttctccggta agagccgcct cgtctacttt aagattatgg
    13141 gagagtatta cccgtgagtc agcagggatt ctctctcctt cttgaagaat aagaatatca
    13201 cccggcacca gttccgtatc cgaaataata atctctttac catctctaag tacagtggcc
    13261 ttagtttcga cgaatttctt gagcgcaagc aaggtgttct gggctttgcc ctcctgaatc
    13321 gtcccgacaa tggcattgaa gagaaggatg gccagaataa ttgaaccgtc gaccacctct
    13381 cccatcgcga agactataat actggcggct accagtatgt aaatgagcgg gctttgaaat
    13441 tgacgcagga aaatagtaaa aatgccgtcc accttggcgt cgggcaattt atttaatcca
    13501 tactgcttca atcgcagggt tacttcctga ctatttaaac cgcgttcgct tgaatgtaat
    13561 tcttcaataa cggcagttga tgattttgta tgccaaagat tattcataaa tttttatttt
    13621 aatactccag cgatttctcg gctggagaga tattgcccag aaccttgtgt ggaaagtagt
    13681 tccaggcatc ggttaccaat ttaaaaacat agctcaattc caaaacgtct ccgccctga
//
LOCUS       DAOQSA010000023        13149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_3799_length_13149_cov_7.303345, whole genome shotgun sequence.
ACCESSION   DAOQSA010000023 DAOQSA010000000
VERSION     DAOQSA010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13149
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3799_length_13149_cov_7.303345"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(365..523)
                     /locus_tag="PKK65_03110"
     CDS             complement(365..523)
                     /locus_tag="PKK65_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81700.1"
                     /translation="MIFKPVLFDSDQKPQGNVDADLVLQAMIEFDNFNQAIIVSSDGD
                     FYCLVKYL"
     gene            complement(748..1317)
                     /locus_tag="PKK65_03115"
     CDS             complement(748..1317)
                     /locus_tag="PKK65_03115"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNU81701.1"
                     /translation="MTKIKNIPNKERVCIYIDGSNFYNYLKDAEINFPRGKKFDFLKF
                     IELLAGEREVVSKCYYTGIFRNIDNSEKSEKLVKNQQRFLSYLEKIGFTIKRGRILCD
                     GGRPREKGTDVRIAVDLVVGAIDSLYDSAILVSSDTDLIPALDYVKYKNKKIEYVGFS
                     HAPSYALIKHSHFRRLLLPEDIKKLEDEW"
     gene            complement(1748..1834)
                     /locus_tag="PKK65_03120"
     tRNA            complement(1748..1834)
                     /locus_tag="PKK65_03120"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1798..1800),aa:Ser,seq:gga)
     gene            complement(1856..2575)
                     /locus_tag="PKK65_03125"
     CDS             complement(1856..2575)
                     /locus_tag="PKK65_03125"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNU81702.1"
                     /translation="METKKTVRVILIFIIATLVVFSLILFFNNHLEEKGQSLTPVENS
                     LEIAGGHQSYVAVNMPIINDDDCLSGHGDDLDIIVYEDYGDIFSADFYDSLEQARLDF
                     GDKIRIAYRPFNFNHTDLSSAAAVAVRCAFDQGKGDDFRGKLFQAVRANQFNHDKLSA
                     LAGEIGLNQEDFQECLTNPQKKEKIEKMFLAAANFSVYGAPTVFIGEEMIVGARPYSN
                     FTDSNGDEIEGLKQVIARQLK"
     gene            complement(2598..4064)
                     /gene="gatA"
                     /locus_tag="PKK65_03130"
     CDS             complement(2598..4064)
                     /gene="gatA"
                     /locus_tag="PKK65_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNU81703.1"
                     /translation="MDGQMFSVASARQELDKGNVSSLELTRACLARLQAIDSELHSCL
                     LVDEKGALAAAVEADERLRKGERGSLLGIPYLVKDILMTKGLRTTAASKILENYTAPY
                     DATVIRKLKKAGAVMLGKTNLDEFAHGSSTENSAFGPTKNPWDLSRVPGGSSGGSAAA
                     MAAGLCLFALGTDTGGSIRQPASFCNLVGLKPSYGRVSRFGLLSMTSSTDTIGPLTRT
                     VEDAAMVLELIAGRDTKDATTLSLPVGDYHLPLSQGFKGLKIGVIPSYFEDGLDEGLR
                     TEAIRALEIFKKEGAEIINLDLPYNQYSVPVYYVITPSEVSSNLARFDGIGWGRAEEG
                     ADNLLSFYKATRGHGFGPEAKRRIMLGTFALSAGYAEAYYKKAQAVRELIKLDFAQAF
                     QKVEVIFTPTSPHAAFKLGEQVNNPLAMYLEDIYLAGASLAGLPAISVPGGFTEIDGQ
                     ELPFGWQFIGPMAQEASVLRAAAAWEKINNYQERRPNL"
     gene            complement(4066..4344)
                     /locus_tag="PKK65_03135"
     CDS             complement(4066..4344)
                     /locus_tag="PKK65_03135"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF014716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNU81704.1"
                     /translation="MQFKEADIAHMADLARLELSAAELASYGAQLSAITAYIEQLQKV
                     KTSSVALAPILYNIWREDVVEDWEVEENGQALSQGKLEQGLLKVKRVL"
     gene            complement(4443..6557)
                     /gene="ligA"
                     /locus_tag="PKK65_03140"
     CDS             complement(4443..6557)
                     /gene="ligA"
                     /locus_tag="PKK65_03140"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNU81705.1"
                     /translation="MDKKIAKNRIEKLKQEIDYHRRQYHVYDRESISAAALDSLKMEL
                     FRLENEFPEFISADSPTQRVGGEALAKFQKVTHRRPMISLYDAFSEEDMRDWEKRNIN
                     YLAERGESLSSLYYCELKLDGLAANLRYEKGIFVQGATRGDGQIGEDITLNLRTIESL
                     PLRLFKPAEKELVALGLKEKDIKNLFYLLAEGVIEVRGETIMSKQTLAALNKQYALEG
                     KPLLANTRNGAAGSLRQLDPQVAAERHLEFYAYDLILGDYERGEIVLSRAAADKLVEL
                     LGFKRVRDNKLCHGLAEVFVFQRYWEKNKDKLPFFIDGVVVKYDDLSLWPRLGTVGKA
                     PRYMVAYKFSAEQATTKLIDVVWQVGRTGVLTPTAILEPVKVGGVTIGRSTLHNLDEI
                     ERLGLRLGDTVIVERAGDVIPKVVAALPNLRTGKEKVIQAPVHCPRCGAEISRLGDDV
                     AYRCLNKNCYAVALRRLGHFVAKGALDIEGLGPKIVEQLVNEGLLEDVADIFALRRED
                     LIGLNGFAEKKAEKLVAAIKSRQEVALSRFLFALGISHIGEESAQTIAVYLSKFLSSP
                     IGRPQELLRVAKKCRLDDWSALADIGPVVAASLFNFWHSQETAALFTKFEKSGLSLLL
                     PLPASGALAGKTFVLTGSLNSLTRQEAKDKIKAKGGKTQESVSRLVDYLVVGEAPGSK
                     LREAEKLGVKILTETEFLKLIQ"
     gene            complement(6644..6880)
                     /locus_tag="PKK65_03145"
     CDS             complement(6644..6880)
                     /locus_tag="PKK65_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81706.1"
                     /translation="MKKELIFPLIAGLILGILVMLFWQLNTKINTSSARLSQLEQATN
                     ANTQAVSEIVSFINQATGQNGAANTQTPTTTPAQ"
     gene            complement(6945..7151)
                     /locus_tag="PKK65_03150"
     CDS             complement(6945..7151)
                     /locus_tag="PKK65_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81707.1"
                     /translation="MKKSFVGPLLTILVIVVLAGLYAYMYYQVNRQEKKMTELQTTIA
                     KDSQTVAGVVNFINASLQNAQPQK"
     gene            complement(7401..7772)
                     /locus_tag="PKK65_03155"
     CDS             complement(7401..7772)
                     /locus_tag="PKK65_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81708.1"
                     /translation="MKKILFFLAIASLIFGSCGKDAGSSPSSFSFTVNSSTFTVDASA
                     LPTATNFLVSLVSSESASSAFSDEFYLGGKTKSLSAEKITLCNDAYFSIIAFDSEGKY
                     VTQNFFPFVEGGQTLTLSDEE"
     gene            complement(7897..>8706)
                     /locus_tag="PKK65_03160"
     CDS             complement(7897..>8706)
                     /locus_tag="PKK65_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81709.1"
                     /translation="SDGAGAGIWATDNGVTGEGTVGFIPYWNTAKSLRDSNIYYDQNN
                     IGIGVTSPGAKLDVNGSLKTNSSVTFSALSGGGSRIVTVDNSGTLGATTNPIPSGTTG
                     QTLRKSTEAWEASSFLWNSGSTVGINYARDACDYAIKLAVNGIIETTGLKIDEVLPTG
                     PACSDSGNISFRSSVMEFIANSGFTFNAPISVTGKINATTGFAYNGTAGLESSFAANT
                     VLTGVSVWGGIITAATPVTGLTVTVSVRNAGNTGSCNLVFTKGILTSETCP"
     assembly_gap    8707..8806
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8807..10083)
                     /locus_tag="PKK65_03165"
     CDS             complement(<8807..10083)
                     /locus_tag="PKK65_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81710.1"
                     /translation="MIASKSKLFAAVVVFSLTAFNLYPLAAAQAKTINLGASGDVVSI
                     PGTILNSNLAGTGNRCLYVDASGNVSVKGFDCGSATGGDNLGDHTATQNIKLNSFWLS
                     GDAGNEGVYVDSAGGVGINTSSIPAGLSLSVNGHMQTNGVLTLANVVYVGGGNRMITV
                     DNAGVLGAAAIPTDTDIYWTGTTGGGFNAATARTSLGLGSLATLSSVSNAQINDVAWS
                     KITNFPSACSAGQYVSAVGSTLTCSTPAGGISGSGTANYVTRWTSGSALGNSVIYDNG
                     TNVGVNNTSPGAKVQINAAASNEGLRIVSASDWSPLNIRNSANTADIFRVDQAGSLAV
                     GSIPWARLTSYPSACSAGQYVSAVGGTLTCSTPTDTNTDVYWTGTSTNLVAATARTSL
                     GLGSLATLNAVSGGASGTITDATITDADISATA"
     gene            complement(10152..11687)
                     /locus_tag="PKK65_03170"
     CDS             complement(10152..11687)
                     /locus_tag="PKK65_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81711.1"
                     /translation="MAKRKTAKTKACWWRYRLSRKLFWPLLIVLFLFFALSVFNFISL
                     LAVQRDLKANLEKIEAGTIVQTESLPGAYHSYRHLDLSAPASATASVTPVVSPLLATA
                     GLIKGENFVPLSSEQMLKGTLSSFGDSFSGMAYINQAETDMFWDESITAFVFPPLYEI
                     HEQGACSAPDCGLSRSSTDPISICLSAGCLRKTEDLKLFFNDRELQLPPAIRNEELNS
                     ITLFALRNSWLIGLVSGPVTAARGWVYRFDGLSFSPLITDDTPLQITPRFQRGGGRIA
                     FGGDDDDFLVLYAGYDGRAFRFRGSNQEDISKFFGLRVTDGGFWPQILKIGDKQDAIF
                     YICSLTENKPKVIKIWSKDDFNSGGALDFSSLFFRGKLNPDSILCGLSDSASKSLMIA
                     ANSQGFYSLWLVRDLGFDNSRSRQVISTNLNRRASSSVKAAVIAETGVESLGSARGGG
                     VSFSMANSAGNFENTPPFLWHAFESEGKELYWRFEAEPDPTNPDYSPWFDNINRLDYL
                     LSD"
     gene            complement(11847..12542)
                     /locus_tag="PKK65_03175"
     CDS             complement(11847..12542)
                     /locus_tag="PKK65_03175"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HNU81712.1"
                     /translation="MDNTNKPAGESKNKKLLVILGLVLVVAIIVVAAVTSSEQSGNNP
                     ADSQSAGKGETSADIPSDVQPYVPEGGEATTTEGEVAIPEALKEATVAVVGANPIAKD
                     GTVVTTEGVAVKNDAVPMSPEAPQQTPPVAKEELPASAIKLDISATGWNPSEFSVKAG
                     APVTLAITSADDYTHIFMFDDSALSAVAVGVSPHETRAITFNAPSKAGEYAFHCDVPG
                     HAARGEVGKMIVK"
     gene            complement(12708..>13149)
                     /locus_tag="PKK65_03180"
     CDS             complement(12708..>13149)
                     /locus_tag="PKK65_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81713.1"
                     /translation="NNITGVNKLTVNTIDPLYRIKGINYSTFASAIVGGVKEEYVGRI
                     EINQPIGETEYEKVIDFDKVEKGSDLWVWRQVVDFNPDNIQVFLTPYGDFANTYYDII
                     GNRLVFRADKPIEVSYRLIGKRFDWRQWPTKALDQSEKAGLIIH"
BASE COUNT         3966 a         3114 c         2516 g         3453 t
ORIGIN      
        1 gaccttaatt taagtatggg tgatgtaaaa ttatttgtca agtatttgta aaaaagttat
       61 acatagccaa ttttaagtcc tattttcttt tttaatatta cccgagcgac cccttaattc
      121 ggcgtttaac aattcagtcc tagccattgc tttttttaat ttatctatgc tatcctaata
      181 atagaatttc tattaaaaag cacctcttaa agttcatctt taagcggtgc ttttttcttt
      241 agggacatat tcaagcttat tcctcaggtt gtccataaat actatcttct cttttgccgt
      301 cttttttaat aattttgagc aattctttac atccggacta atcaccctag acaatttgcc
      361 tttctcataa atatttaacc aaacaataaa agtcaccatc gctagaaaca ataatcgcct
      421 ggttaaaatt atcaaattct atcattgctt gtaaaaccaa atcggcgtca acattgcctt
      481 ggggtttttg atcactatca aataaaactg gcttaaaaat caaaatataa ctaatatcaa
      541 aatgtaaata tccgaactaa acaattttta tttaatgtta actataatac catgcaattc
      601 tctaaaaaat ccctgtctcc tgccgccggt tagaaccatt ttgaaacata atatttatgc
      661 ttatgtataa caaaaaacag tcaaccaaga ctgctttttc ctaacggtgg agagggtggg
      721 acgttgttcg tataatatta agcaaaatta ccactcatct tcaagctttt taatatcttc
      781 tggcaacaat aatcttctaa aatgagaatg ctttattagc gcatacgatg gggcatgaga
      841 aaaccctaca tattcaattt ttttattctt atatttaaca taatctagtg ctggtattaa
      901 atctgtatca gagctaacta aaatagcaga atcataaaga ctatctatcg cccccaccac
      961 caagtcaact gctattctaa cgtcagtacc tttctctcga ggtcttccgc catcacataa
     1021 aatccttcct ctttttatag taaatcctat tttctctaaa tatgacaaaa acctttgctg
     1081 attctttact aatttttcac ttttttctga attatcaata ttcctaaaaa taccagtata
     1141 ataacactta gaaaccactt ctctttcgcc agctaacaat tctataaatt ttaaaaaatc
     1201 aaatttcttt ccccttggaa aattgatttc agcatctttt aaataattat aaaaattact
     1261 cccatctata taaatacaaa ctctttcttt atttggaata ttttttattt tagtcataaa
     1321 tacactatat cataatattg gatataaaaa aactgccctt atcagcgccg aagcgctaat
     1381 agtggcagtg atcatattac atttaatata cattataaaa aaaagaaagt caacaaaaaa
     1441 atccatactc agtaacatgc caactttgga acttactatt tttaatttaa aaatagctaa
     1501 aatattatta ttttctaata ttttttactt aatcctctta acaaaaataa gctagtaata
     1561 gcttattttg ttactgcgaa gagacagaaa ttaatcgccc tcgcctcgcc tcaaaatagg
     1621 cgaggctcac ttgtccatgg ttggcgtcca aatatccccc ggatatttgt ccgacgctcg
     1681 tccgctcgtt ccggcttccg ccgccgctcg cgtcttgctg ccaacctgtt tgaatccctt
     1741 ttctgagcgg agagtaaggg atttgaaccc ttgataccgg ttagggtata cccgctttcc
     1801 aagcgagcgc actcgaccac tatgcgaact ctcccagagg ctgtctgaaa cagccttatt
     1861 tcaattgtct agcaataact tgcttcaaac cctcaatctc atcgccatta gaatctgtaa
     1921 aattactata agggcgagcg ccgacaatca tttcctcgcc aataaaaacg gtgggcgccc
     1981 cataaacgga aaagttggcg gcagcaagaa acatcttctc tattttttct tttttttggg
     2041 gattagtcaa gcactcttga aaatcctcct gatttaaccc gatttctccc gccaaagcgc
     2101 ttaacttatc atgattaaat tgattcgcac gaacagcttg aaaaagtttc ccccgaaaat
     2161 cgtccccttt accttggtca aaagcgcaac ggacagccac cgccgcggcg gaagataaat
     2221 cagtatgatt gaaattaaaa ggacgatagg caatccttat cttgtcgcca aaatcaaggc
     2281 gagcctgttc caaggaatca tagaaatcgg cgctgaaaat atcgccataa tcttcataga
     2341 cgatgatgtc taaatcatcc ccgtgcccag aaagacaatc atcatcatta attataggca
     2401 tattaacggc gacgtagctt tggtggccgc cagcaatctc aagactattt tccaccggcg
     2461 tcaaactctg acccttttct tccagatgat tattgaaaaa caaaataaga ctaaacacga
     2521 ctaaggtggc aatgataaaa attaaaataa ctctgactgt tttttttgtt tccataataa
     2581 tgggtagaga cggacgctta aagattaggg cggcgctctt gataattatt aattttttcc
     2641 caagcggcgg ccgctcgtaa aacagacgct tcttgagcca tcggaccgat aaattgccaa
     2701 ccgaaaggca attcctggcc gtcaatctcg gtaaagcctc cgggcacgga aatagccggt
     2761 aaacccgcta aagaagcgcc ggctaaatat atatcttcta aatacatcgc caaaggatta
     2821 ttaacctgct ctcccaactt aaaggcggca tggggtgaag ttggcgtaaa aatgacctcg
     2881 actttttgaa aagcctgagc aaaatcaagc ttaataagct ccctaaccgc ctgcgccttc
     2941 ttatagtagg cctcggcgta accggcgctt aaagcgaagg tgccgagcat tattcttctt
     3001 ttcgcctccg gaccgaaacc gtggccgcga gtggctttat aaaaagacaa aagattgtct
     3061 gcgccctctt ccgcccggcc ccagccaatg ccgtcaaaac gggccagatt ggaactgacc
     3121 tcgctagggg taataacgta atatacaggc acgctgtatt gattataagg taaatcaagg
     3181 ttaattatct ctgctccttc ttttttaaat atctctaagg cccttatcgc ctcagtgcgc
     3241 aggccttcat cgaggccatc ctcaaaatat gagggaataa caccaatttt taaaccctta
     3301 aaaccttgac ttaaaggtaa atgataatct ccaacgggaa gggataaagt ggtggcatct
     3361 ttggtatctc gaccggcaat aagctctaaa accatggcgg cgtcttcaac tgtgcgcgtc
     3421 aaagggccga tagtatcagt ggaggaagtc attgacaata agccgaaacg agaaacacgt
     3481 ccataactag gttttaagcc aaccagatta caaaaactgg ctggctgacg aatggacccg
     3541 ccggtatcag taccaagggc gaacagacat aaaccggccg ccattgccgc cgcactgcca
     3601 ccggaagaac caccaggaac ccggcttaaa tcccaaggat ttttcgtcgg gccgaaagcc
     3661 gaattttccg tggaagaacc gtgagcaaac tcatcaagat tggtcttgcc cagcattacc
     3721 gctcccgctt tttttaactt tctaataacg gtagcatcgt aaggagcggt ataattctcc
     3781 aaaattttgg aagcggccgt agtccttaaa cccttggtca tcaaaatatc tttaactaga
     3841 taggggatac cgagcaaaga accgcgctcg ccctttctca gacgctcatc cgcctcgacc
     3901 gccgccgcta aagcgccttt ttcgtccacc agtaaacaag aatgaagctc agaatcaatt
     3961 gcctgaaggc gcgccaaaca agctctcgtt aattccagag agctgacgtt tcccttatct
     4021 aattcttgac gcgctgacgc cacgctaaac atctgtccgt ccatattata aaactctttt
     4081 tacctttaaa agcccctgct ctaactttcc ttgactcaaa gcctgtccgt tttcttccac
     4141 ctcccaatct tccacgacat cttcccgcca aatattatac aaaatcggcg ccagggcgac
     4201 tgaggacgtc tttactttct gtaattgctc aatataggcg gtgattgccg acaactgggc
     4261 gccataagac gccaattccg ccgcgcttaa ttctaagcgg gcgagatcgg ccatatgagc
     4321 aatatcggct tctttaaatt gcataaaagt ttagaaagaa gttagggcga ttttgctaaa
     4381 ataattgcct actttcaaac atttaactat aaaaataatt attgatactc ttctaaaaga
     4441 ttttattgga ttaattttaa aaattccgtt tcggttaata tttttacgcc caatttttcg
     4501 gcttcccgca atttagaacc cggcgcctcg cccaccacca aataatccac caaacggcta
     4561 acactctcct gagttttacc gcccttggct ttaattttat cttttgcctc ttgtcttgtc
     4621 aaactattca aggagccggt tagaacaaaa gttttgccgg ctaaagcgcc gctagcggga
     4681 agaggtaaaa gcaagctcag accggatttt tcaaatttag taaataaggc cgcggtctct
     4741 tggctatgcc agaaattaaa taaactggcg gcgacgactg ggccgatatc agctaaagcg
     4801 gaccaatcat caagacggca cttcttcgcc acccttaata attcttgagg acggccaatt
     4861 ggggatgaca aaaatttaga taaatagaca gcgatggtct gggcgctttc ttcgccgata
     4921 tgggaaatac ccagagcaaa taaaaatcga gacaaggcca cctcttgacg cgacttaatg
     4981 gccgccacca gtttctccgc cttcttctcc gcaaaaccgt taagaccaat caagtcttcc
     5041 cggcgcaaag caaaaatatc ggccacatct tccaacaagc cttcattgac taattgttcc
     5101 actatttttg gtcccaagcc ttcaatatct aaagccccct tagcgacaaa atgtcccaag
     5161 cgccgcaacg caacggcata gcaattctta tttaaacaac gataggcaac gtcatcgcct
     5221 aggcggctaa tttcagcgcc gcaacgcgga caatgaacgg gcgcctgaat tactttttct
     5281 ttgccggtac gcaaattggg caaagccgcc acgactttcg gaatgacatc ccccgccctt
     5341 tcgacaataa ccgtatcgcc caaacgcaga cccaaacgtt caatttcatc taaattatga
     5401 agcgtggagc ggccaatggt gacgccgcca acttttaccg gctctaaaat cgccgttggc
     5461 gtcaaaacgc ccgtgcgacc aacttgccag acaacatcaa tcagcttagt cgtcgcctgt
     5521 tccgccgaaa atttataggc caccatgtaa cgcggcgcct tacccaccgt tcctaagcgc
     5581 ggccataaag acaagtcatc atattttacc accacaccat cgataaaaaa gggcaactta
     5641 tccttatttt tttcccaata ccgctgaaaa acaaaaactt ccgccaaacc gtggcacagc
     5701 ttattatccc tcactctctt aaagcctaaa agttcgacta atttatccgc cgccgcgcgg
     5761 ctaagcacta tttctccgcg ttcataatcg ccgagaatta agtcataggc gtaaaattct
     5821 aaatgacgtt cggccgctac ttgcggatca agctgacgca gagaaccggc ggcgccattt
     5881 ctggtattag ccagtagggg tttgccctca agagcatatt gcttattcaa ggccgctaac
     5941 gtttgcttgc tcatgattgt ttcccctctg acttcaatta cgccctccgc taaaagataa
     6001 aacaaatttt ttatatcttt ttctttcaaa cccaaagcaa ctaattcttt ttctgccggc
     6061 ttaaagaggc gcaaaggcaa gctttcaatc gtgcgcaaat tcaaagtaat atcttcacca
     6121 atctggccgt cgccgcgggt agcgccttgg acaaatattc ctttttcgta acgaagattg
     6181 gcggccaagc cgtccagctt gagctcgcaa tagtataaag atgacagcga ctctccgcgc
     6241 tcagccaaat aattaatatt tctcttttcc cagtcacgca tatcctcttc agaaaaagca
     6301 tcataaagag aaatcatggg acggcgatgc gttactttct gaaatttagc taacgcttcc
     6361 ccgcccaccc tttgcgtagg cgagtcggcg ctaataaatt caggaaattc attttctaaa
     6421 cggaaaagct ccatcttcaa actatcaagc gccgcggcgg aaatactttc tcggtcatag
     6481 acatgatact gacggcgatg ataatcaatc tcctgcttta atttctcaat cctattttta
     6541 gctatttttt tatccataaa gcaaatctga ctatcatatt atagcatatc aagagcaaca
     6601 aaaaaacgct ctttccagag cgtttttctt aaaaaacaaa agattattga gcgggagtgg
     6661 tcgttggcgt ctgagtgttg gcagccccgt tctgaccagt cgcctgatta atgaaactga
     6721 cgatttcgga aaccgcctga gtattggcat tggtcgcctg ttctaattgg cttaagcggg
     6781 cgctgctggt gttaatttta gtatttaact gccagaataa catcactaaa ataccgagaa
     6841 tcaagccggc aatcaaggga aaaattaatt cttttttcat atgtttttat attgctcgcg
     6901 gccaagcgct caagtaagcg gcatcggctt aggagtgctt aaatttattt ttgtggttga
     6961 gcgttttgga ggctggcgtt aataaaatta accacgccgg caacagtctg tgaatccttg
     7021 gcaatcgtgg tctgcaattc ggtcatcttc ttttcttggc gattgacctg ataatacatg
     7081 taagcataga gccccgctaa gaccacaatg actaaaattg tcagcaaggg accgacgaat
     7141 gattttttca tattagagcg cgggaagcgc tggctttcgc taatggagcg agatggtcgc
     7201 cgcttcgtag ttagcataat tattttttat taatttatat ttaataaaat caaaaataca
     7261 agactaaaga ctgattatta atattatagc ataagctaaa aatggaggca atattaaagt
     7321 atatattaaa gtataaataa aaaggaccaa acggataata tgctttttgc ataaatcggt
     7381 ttggtcctga agatgcggtg tcattcctca tcagataagg ttaatgtctg acccccttca
     7441 acgaagggga aaaaattttg tgtaacatac ttaccctcag aatcaaaggc gattatggaa
     7501 aaataagcat cattacacaa ggtaattttt tcggcagata aacttttcgt tttaccgcca
     7561 agataaaatt catcgctaaa agcactgcta gcgctttcag agcttacaag cgataccaaa
     7621 aaattagtgg cggttggcag ggctgatgcg tcaactgtaa aagttgaaga gttaaccgtg
     7681 aaggaaaaag atgagggcga cgaaccggcg tcttttccgc atgatccaaa aatcaaagat
     7741 gcgattgcta aaaaaaacaa aatttttttc atgtgtcttt atttgagagt tataaaagaa
     7801 ctaattatta ttaatataat acttttatat aattttgtca atacatactt atcaacaccc
     7861 cttttaccta tcaaaaaatt taatatcagc taaaaattag ggacaagtct cagaagttaa
     7921 aataccttta gtaaagacta aattacaact tcccgtattg ccagcgtttc ttactgacac
     7981 tgtcactgtc aaaccggtaa caggggtagc ggccgtaatg ataccacccc aaacgctaac
     8041 gccggttaaa actgtgttgg cggcaaaaga agattctaaa ccggcagtgc cattataggc
     8101 aaaaccggta gtcgcattaa tcttgccagt aactgaaata ggggcgttaa aagtaaagcc
     8161 gctattagcg ataaactcca taacgctaga acggaaagaa atattgcctg aatctgaaca
     8221 agcgggtcca gtcggcagaa cttcatcaat ctttaagccg gtagtttcaa taataccgtt
     8281 aactgccaac ttgattgcgt aatcacaagc atctcgtgca tagttaatcc cgaccgtcga
     8341 accggaattc cacaagaaag aactagcctc ccaagcctcg gtgctcttac gtaaagtttg
     8401 accggtggtg ccgctaggaa tagggttagt ggtagcgcct aaagttccag aattatcaac
     8461 tgtcacaatt ctagaaccgc cgccacttaa agcagaaaat gtcaccgatg aattagtttt
     8521 caggctaccg ttgacatcca acttagcgcc aggggaagta acgccaatac caatgttatt
     8581 ttgatcatag tagatattag aatcccttaa actcttggcg gtattccaat agggaataaa
     8641 gccgacggtg ccttctccag ttactccgtt atctgtcgcc caaataccgg caccagcgcc
     8701 atcagannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttag cggtagcgct
     8821 aatatcagcg tcggtgatgg tagcatcagt gatggtaccg ctagcgccac ctgaaactgc
     8881 gtttaaagtc gctagcgaac caagacccaa agaagtgcgg gcggtagcag ctactaaatt
     8941 agtggaagta cctgtccaat agacatcagt attagtatca gtgggggtag aacaagttaa
     9001 agttccgccc accgcggaaa catattggcc agccgaacag gcgctgggat aagaagtgag
     9061 acgagcccaa ggaattgaac ccacggccag agagccggct tggtccactc gaaaaatatc
     9121 ggcggtattg gcgctattgc gaatgtttaa aggactccaa tcagaagcgg aaacgatccg
     9181 caaaccttca ttgctggcag cggcgtttat ctgcaccttg gcgccaggac tagtattatt
     9241 aacaccgaca ttggtgccat tatcatagat aacggaatta cccaaagcgc taccgcttgt
     9301 ccaacgggtg acatagttgg ctgtaccaga accgctaatc cctccggccg gagtggaaca
     9361 agtcaaggtg ctgccgaccg cggaaacata ttgtccggcc gaacaggcgc tgggaaagtt
     9421 ggtaattttt gaccaggcaa catcattaat ctgggcattg ctaaccgaac ttaaagtcgc
     9481 tagcgaacca agacccaaag aagtgcgggc ggtagcggca ttaaagccgc cgccggtagt
     9541 gcccgtccaa taaatatcgg tatcggtcgg aatagcggcg gcacctaaaa cgccggcatt
     9601 atcaacggta atcatcctat taccaccacc aacataaacg acattagcaa gagtgagaac
     9661 accattagtt tgcatatgac catttaccga caaactaaga ccggcgggaa ttgaactagt
     9721 attaataccg acaccgccag cgctgtcaac ataaacgcct tcattgccgg catcacccga
     9781 cagccaaaaa gagttgagct taatattctg agtagcggta tgatcgccaa gattatcgcc
     9841 gccagtggcg ctgccgcaat caaaaccttt aaccgaaacg ttaccagaag catcaacata
     9901 aagacagcga ttacccgtac cggccagatt actgttaaga attgtgccgg gaatagaaac
     9961 cacgtccccg gaagctccta gattaatggt tttagcttgc gccgccgcca aaggatatag
    10021 attaaaagcc gttagggaaa aaacaacaac agccgcaaat aatttgcttt tagaggctat
    10081 catataaatt ttgattaata tttatcacac tgataattat agcatttatt cgatagtaat
    10141 cctagatatg actagtccga taaaaggtaa tcaagacgat tgatgttgtc aaaccaagga
    10201 gaataatctg gattagtggg atccggctcc gcctcaaaac gccaatataa ctccttgccc
    10261 tccgattcga aagcatgcca caaaaaaggc ggcgtgtttt caaaattacc ggcgctatta
    10321 gccatagaaa aactaacacc gccgccccgc gccgaaccga gagattccac tccggtttcg
    10381 gcaatcaccg ccgccttgac agacgacgac gctctgcgat taaggttagt ggaaattact
    10441 tgtcgagacc gactattatc aaaacccaaa tcgcgcacca gccagagaga ataaaagcct
    10501 tgactattag cagcaatcat taagctcttg ctggcagaat cactaagacc gcacaaaatg
    10561 ctatcggggt ttagctttcc tcggaaaaat aaagaagaaa aatccaaagc gcccccgctg
    10621 ttgaagtcat cttttgacca aatttttatt actttaggct tattttcagt caagctacaa
    10681 atatagaaaa tggcgtcttg cttgtcccca attttaagaa tctgcggcca gaaacccccg
    10741 tcagtcacac gtaaaccaaa gaatttgctt atatcctctt gattagaacc gcgaaaacgg
    10801 aaagcgcggc cgtcatagcc ggcatataac accaaaaaat catcatcatc gccgccgaaa
    10861 gcaatccgcc ctcctccccg ctggaaacga ggggtaatct gtaaaggggt atcatcagta
    10921 attaatggcg aaaaagacag accgtcaaaa cgataaaccc aaccccttgc cgccgttact
    10981 ggtccgctga ccaaaccgat taaccaggaa ttacgcaaag cgaataaagt aatactgttt
    11041 aactcttcgt ttcttatcgc cggcggcaat tgtaactccc ggtcattaaa aaataatttt
    11101 aaatcttccg ttttacgcaa acaaccggcc gaaaggcaaa tagaaatagg gtcagtggat
    11161 gaccgtgaca agccgcaatc aggcgccgaa caagctcctt gttcatgaat ttcataaaga
    11221 ggaggaaaaa caaaagcggt aatactttca tcccaaaaca tatcggtctc cgcttgatta
    11281 atataagcca ttccggaaaa actatcgccg aaactactta atgttccctt taacatctgt
    11341 tcgctcgaca acgggacaaa gttctcgccc ttaatcaagc cggctgtcgc caataacggc
    11401 gacaccaccg gtgtgactga cgcggtcgcg ctggcagggg cgcttaaatc taaatggcga
    11461 taactgtgat aagcccccgg caaagactca gtctggacaa tcgtacctgc ttcgattttc
    11521 tccaaattgg ccttcaaatc acgctgcacc gccaagagag aaataaaatt aaaaacggat
    11581 aaagcaaaga ataaaaagag gacaatcaat aagggccaga ataattttct cgacaaacga
    11641 taacgccacc aacaagcttt ggtcttggct gtttttcttt tagccataat aaaaattaag
    11701 ctagatattt atgcctttat tttaatataa aaactattct ttgtctaacc atagacataa
    11761 aaagacagcc gcccgattcc aagggaggct gtctttaatt ttctattatc aaactctagc
    11821 cggaaacgga aaagttgata agcaagttat ttaacaatca tcttgccgac ttcgccgcga
    11881 gcggcatgac cggggacatc gcaatggaaa gcatattcac cggctttaga cggagcattg
    11941 aaagtgatgg cgcgggtttc atgaggagaa acgccgaccg ccaccgcgga taaagccgaa
    12001 tcatcaaaca tgaaaatatg ggtataatca tcggcggaag taattgctaa agttacaggc
    12061 gctccggctt tgacggaaaa ctcactaggg ttccaaccgg tggcagaaat atccaattta
    12121 atggcactgg ccggtaattc ttctttggcc accggcgggg tctgttgagg cgcttctggt
    12181 gacatcggga cggcgtcatt tttgaccgcc accccctcgg tagtcaccac ggttccgtct
    12241 ttagcaatcg gattagcgcc aaccacggcc acagtcgcct ctttcaaggc ctcggggata
    12301 gctacttctc cctcagtcgt agttgcctct cctccttcag gaacataggg ctgaacatca
    12361 gacggaatat cagcgctcgt ctcgcccttc ccggcagact ggctgtcagc gggattattg
    12421 cctgattgtt cactgctcgt cacagcggcc accacgatga tggcgacgac gaggaccaag
    12481 cccaagatca ctaaaagctt tttgttcttg gactcgcccg ccggcttgtt tgtattgtcc
    12541 atattttttg cttggggccg ccaggagaaa actcagccag cgccgccaat ttattaacgg
    12601 atgattgtct aaactaagtc ttaatccttg ttaaggactt agaaccagca gacaaaacat
    12661 ccaaattata atgattactt atattataac gtaaataagc aagtctatca atgaatgata
    12721 aggccggctt tttccgattg atccaaagcc ttggtcggcc actgccgcca atcaaaacgc
    12781 ttacctatca atctatatga aacttcaata ggcttatccg ccctaaaaac caaacggtta
    12841 ccgataatat cataataagt attagcaaaa tcgccataag gggtaagaaa aacctggata
    12901 ttatctggat taaaatcaac cacttgacgc caaacccaga ggtcacttcc cttttcaact
    12961 ttatcaaaat caattacttt ctcgtactcc gtttccccta tcggctgatt aatttcaatc
    13021 cgaccaacat attcttcctt aacgccgcca acaatagcgg aagcaaaagt tgaataattt
    13081 atacccttaa tacgatatag aggatcgatg gtattgacgg tcaacttatt gaccccagta
    13141 atattgtta
//
LOCUS       DAOQSA010000024        10978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_5197_length_10978_cov_7.144649, whole genome shotgun sequence.
ACCESSION   DAOQSA010000024 DAOQSA010000000
VERSION     DAOQSA010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10978
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5197_length_10978_cov_7.144649"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PKK65_03185"
     CDS             complement(<1..247)
                     /locus_tag="PKK65_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNU81714.1"
                     /translation="MKIFFWLKKIWEIMDYACGQRLAPQLAGIIDKLVFFKELNIPAT
                     TQKKLKHISSTTIDDRLKRFKNELRLKINSTTRPGSLL"
     gene            884..1201
                     /locus_tag="PKK65_03190"
     CDS             884..1201
                     /locus_tag="PKK65_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81715.1"
                     /translation="MIGKKTEISAKLFHFNRITGSVDVIAEMDKDGYRPATLMELLIL
                     GILFPELQRQFPIVALGSIWHHKDGDYYVPFLYASGSRRELGLLWFDLVWDVRFRFLG
                     ARK"
     gene            1710..2885
                     /locus_tag="PKK65_03195"
     CDS             1710..2885
                     /locus_tag="PKK65_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81716.1"
                     /translation="MTKKEAQYNIIVNVLIKTGVFSISKIESQTSISRSWLSKILRHL
                     VNENILVMTAESGANVKYKLANNKKALEFLFHDLDSLTIEQLAKVWGISVQTAKNNIK
                     KFVDSGDILKQGLPPQKIIYKLVSQDNVDDLSDEQKQVLNKYYIYVTPDGQLLKGVSG
                     FLYWAKNKSGRLAVNSLAEEYLKIRQKFYNDKNKVFLIDATEKMRQVFKDDIKLIKVF
                     HRDFDSLPVFGKTYLSQMIRIAKAGRTNTAVMNYIVDNIRDGVNYIIKNYNIDAIGFI
                     PPTVMRKTQLMTFISKKLRVDLPIIPINKHTGLVPVQQKSLRDIKDRILNANKTIFIS
                     DSYNYNNVLLIDDVTGSGATLNETAKKLINNGLAKNIYGFTITGSAKANDFDVISEA"
     gene            3151..3675
                     /locus_tag="PKK65_03200"
     CDS             3151..3675
                     /locus_tag="PKK65_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81717.1"
                     /translation="MKIFRIVLIVVSTCFIFFPRLQAQTAAFIINRENKEIVLLPRSG
                     GPGLIIPAGDSVWSFDVSDIYYVYQQIKKSPQVVEKVGFCRLTPLDGAYEFAGDKLPI
                     FVKNNSEQNVLLEFSDMVVSLPAGGSFCADDLVIEPDLTALVYLSGNDSVSAPGFRLL
                     SFTINNGSLSANLW"
     gene            3775..5880
                     /locus_tag="PKK65_03205"
     CDS             3775..5880
                     /locus_tag="PKK65_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNU81718.1"
                     /translation="MFNFLHKKESISPSDEKDPEKKDTIEEPNSTEPILIDNDNNKEK
                     ASSLNPDSLEKLREAVEIQEEMGNHLSLEDTLLVTGTSPEKLPEILAEQDINLEAKGE
                     QVIPEIARVLESLEEDEKSELSGFRRFTNYNSIKAAFIALMLLVKFAPDAQGAEPQDK
                     LKEGVKMGKEVKGELDREPNSDKTYQLSAKDFDDERPEKHKSNDAKPLDIKLEENSRL
                     ASLELTHYFATDKADIINPQAIISDFEAFLSKITPDNAQEIMAADFKLFGSSDERPTS
                     NWKGSNTELTKARLTALDKLLTETLQNYKFDKLPADLAEQIRNKKFFHEMPFSQTGPE
                     EGVIYITDLDNPETGQKYTPEEVASIKANYPEKYKLLLDDCRRISFSATMPQMDFINK
                     MKMRNSEVEKGYEVSPRSPEFLKMNEYDNVYLLFDNSPSVGDSYPYMAQMIEKQNFKE
                     LKVNFATFSNHLDGIKQLDNSVEVAKNIRDTKYFGDTRERALDVAYTALNKMKAEEKN
                     ALFIMTDEDIQAVSWEKIQKARSLAAEKNACVYFYYGDDKHQAIRQVSLDELERAYKE
                     QAMAAIAPKINFLYNAASKRISALESRRQSQENLISRLAAKTNIPAYQKSLLAHQKEL
                     SKINENLTNEKEKLAALMRAWEGGDIEDVFVQQAFYWKVASDTEVQKVRQGIEINVAV
                     NNLGSEVVDLASLSEADGR"
     gene            5988..7781
                     /gene="typA"
                     /locus_tag="PKK65_03210"
     CDS             5988..7781
                     /gene="typA"
                     /locus_tag="PKK65_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNU81719.1"
                     /translation="MEIRNIAIIAHVDHGKTTLTDALMRQTGIAGEEVSMDSNALEKE
                     RGITIYSKNTSIFYKDTKINIVDTPGHADFSSEVERILRSIDCVLLLVDAQEGPMPQT
                     KFVLKKSLELGFKPIVVINKIDKPAARPAEVVERVYELFLDFGANDAQLDFPVIYAAA
                     KQGIAKKELTDASDNLNPLLDLIITHVSLASSEEALSRPLLAQPFNLAYDNFLGRCAI
                     CRLYEGKIKAAQTVFIKDLDGTTRARKITKLFTFKGVNRQEVPEAEAGDIVMIAGLPD
                     IYIGETIAERADQKALPAIKIDEPTIALNFLVNNSPFAGREGKFVTNRQLKERLEKEM
                     EVNVGLKIDFSEPSYYRVFGRGELHIAILLENLRREGYELQVSQPQVIIKDINGVKSE
                     PFEEVTIDIPENLSGMVIEKISKRRGKMLDFKTHNRQSRLTFAVPTRGLLGYRNEFIV
                     DTRGEGILCAQVLGFQPYAGDIYKNNLGSMVSMITGKALAFALWNLQDRGVLYIAPNT
                     EVYEGMVIGNVSKGNDLAVNPIKGKQLTNVRASGSDEAIQLTPPVPLTIEFGLSVMKE
                     DEYLEITPLSLRLRKQHLTEVERQRAKRVKI"
     assembly_gap    7836..7935
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7936..8702
                     /locus_tag="PKK65_03215"
     CDS             <7936..8702
                     /locus_tag="PKK65_03215"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNU81720.1"
                     /translation="GLDIFTDKLPGFEPYHSGCFRDDDKTRVADFKPAPPGEIKDSLI
                     ELLAWIKKNPSAINVFIFHAALYSLHPFKNGNKRVCRVLEHLILRDIAYNQNNLYNLS
                     YYYYQQKDRYYKNLINSLYKHSFNPFVAFASEALYFSALGVIKGVLERKKQEFINNSN
                     LGLPAIKLLKPLVKNGELKFSHFYALSKRKVSRQTFINYLNEALDQQIIIKREQGRNT
                     YYSVAGNYLEVDILKEKLKIAREKLNYLPPDLISYL"
     gene            complement(8808..9908)
                     /locus_tag="PKK65_03220"
     CDS             complement(8808..9908)
                     /locus_tag="PKK65_03220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNU81721.1"
                     /translation="MKILQINKLYYPVIGGIETIVQNIAEKLNQKSEFTVDVLVCQNK
                     GKRQIETVRGVKVYRAASWGKKLGMPLSLDFFQLFKDIKNNYDQIILHYPFPLAALTS
                     PFIPKKKLIIYYHSDIVRQKLGALIFSPFIKYSLKKAHTILVGSHNLINSSPNLKTHS
                     DKCQVLPFGYQADYTPNDYQKAEEVKRQYGKNKVLLLAVGRLVYYKGFAYAIKAMSGV
                     KAQLLIIGDGPEKGRLEKLITDLNLQKNVSIISCQPDLKPFFLAADIFLFPSTERSEA
                     FGLVQLEALAAGKPIINTYLQTGVEEVSLNEVTGLTVKPRNIAALNKAINTLVSAPKL
                     RKKFGQAGQERYHRLYTTEIFIDNLKTILNNI"
     gene            complement(9886..>10978)
                     /locus_tag="PKK65_03225"
     CDS             complement(9886..>10978)
                     /locus_tag="PKK65_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNU81722.1"
                     /translation="SLMKIVINALPYNTYQGIENFLTNLLRAWPHNEDEITVLANEIS
                     AKFLQPLPAYFKLEIIKFKKIDKLSIFIFQQFKLGRLLKKRKTDILFCASLNCPWLYP
                     QKIVTIHDAAPFIIKGESSRFGKIYWLISLFFAKHFSRHLLTVSEFSRQELINKLKIN
                     QNKLSVIYNGAPVIPVINKNQTQTVSPYIIVIGNARKRKNLTTLLAAYNLIKTDIPDL
                     RLIVVGKMDKRMQILANSYQNDRIEFTGFISDENKYQLLSQAQALIFPSLYEGFGLPI
                     LEAQIMGTPIICSNIPVFKEVANTGALFFDPLSASDLAEKIKKIINNPVLAANLKNEG
                     SKNYPRFNWQKSAKKLVDILHLYENSSDK"
BASE COUNT         3351 a         1806 c         2252 g         3469 t
ORIGIN      
        1 tgagcagact acccggtcta gtggtggaat tgattttcaa gcggagttca tttttgaatc
       61 tttttaatcg gtcgtcgatg gtggtcgaag aaatgtgttt taattttttt tgagtcgtcg
      121 cgggaatatt caactcttta aaaaacacta atttatcaat aatccctgct aactgcggtg
      181 ccagtctttg gccacaggca taatccatga tttcccagat cttcttgagc cagaaaaata
      241 tcttcattag tataggtgta gtggctctgg cggttaatga ctttgagtcg gtactcgtgg
      301 cttggagaca aaatcctcac tgcatacttt ctattgtagc cggtattttg acagaactcc
      361 cccagcatcc ccgacttgct ttttttgtcc gccttcagat acctcggttt tagtcgagac
      421 aaactctctt ttcttgaggc aatgctcatt tttgtggtaa ccatagattt gaaccttaat
      481 tttataggtt ttatggttac attttacaat catttgtcga atacccctat ggttacattt
      541 tgcaccattt gattcgaagt aattgaaaaa agatagatat atgatataat ctactcattg
      601 ttttttaaaa taaatcaaat aaaatcatag atcatgaaaa tctggaaagc gcatttacag
      661 aaattaacag cggggatgga aaataaaaaa ttgtccatta gagccaagtt attgaactta
      721 ttaaaggtag taatatcaaa ggtgaaaaag tagtcgatac tgctaacgaa attaagttag
      781 ttgttgatta caccaaaact cttgaacaag ttattgccga aggtaattat aactgggaaa
      841 ataataatat cattacacca aaaattttcc agtttccgca gaaatgatcg gaaaaaaaac
      901 cgaaatatct gctaaattat ttcattttaa tcgtatcacc ggctcagttg atgttatcgc
      961 tgaaatggat aaagatggtt accgcccggc tactttaatg gagcttttaa ttctgggaat
     1021 tttatttcct gagctacaaa ggcaatttcc gattgttgct cttggttcca tttggcacca
     1081 taaggatggt gattattatg tgccttttct ttatgcgagt ggctctaggc gtgagcttgg
     1141 tctcctttgg tttgatcttg tttgggatgt tcgttttcgg ttcctgggag cccgcaagta
     1201 atcgcttgtt ttcttaggct cttggcccct taactaaaaa cccgtttcaa aaatgaagcg
     1261 ggcttttttg aataaaaata tgataagata tttttggata gtaggcaaat ctgtcgaatc
     1321 tgtcagtggt tacggctttt ggcgcccgaa ttctaaaata ttatttaagt aatttatgta
     1381 taattattgg ctaatatgaa gtaaaataaa atatcagagt ggcccactta gttcgtgcct
     1441 cacttacctc gccaagacaa tttagtgtcg gtttaatcta agccccataa ttataggggt
     1501 tttttggtgt taaacaataa ttaaaattta atgataaata aagtcaatat attgtttatt
     1561 atgtactgta tattataaat aatattagta aaaaaatagt atttttaata tattataata
     1621 tatagtataa tacatactaa atattttatt taatattagt atatttaatc ctatttacaa
     1681 tattattaat atatagtata atacatagta tgactaagaa agaggcacaa tataatataa
     1741 tagttaatgt cttgataaaa acgggtgttt tttctatttc caaaattgaa agtcagacaa
     1801 gtatctctcg cagctggtta agtaaaattt tacgtcacct agttaatgaa aatattttag
     1861 tgatgaccgc agaatcaggc gctaacgtta agtataagct ggcgaataat aaaaaggctt
     1921 tggaattttt gtttcatgat cttgatagtt taaccataga gcaattagct aaagtttggg
     1981 gtattagcgt tcagacagct aaaaataata taaaaaaatt cgttgatagt ggcgatattt
     2041 taaagcaggg tttgccccca caaaaaatta tctacaaatt ggttagtcaa gataacgttg
     2101 atgacttgtc tgacgaacaa aaacaagtat taaataaata ttatatttat gttactccgg
     2161 atgggcagct tttaaaaggt gtttctggtt ttttgtattg ggctaaaaat aaatctggac
     2221 gtttggcagt taattctctt gccgaggaat acttaaaaat tcgtcagaaa ttttataatg
     2281 ataaaaataa agttttttta attgatgcga ctgaaaaaat gcgacaagtt tttaaagacg
     2341 acataaaact aattaaggtt tttcaccgcg attttgattc tttaccagtt tttggcaaaa
     2401 cttatcttag ccaaatgatt agaattgcta aggctggacg tactaacacg gcggttatga
     2461 attacatagt tgataatatc agggatggtg ttaattatat tattaaaaat tataatattg
     2521 atgctattgg gtttattcca ccaacggtga tgcgaaaaac acagctaatg acatttattt
     2581 ctaagaaact tagagttgat ttgcctataa ttcccattaa taaacatacc gggcttgttc
     2641 cggtccagca aaaaagttta agagatataa aagatagaat tttaaatgcc aataaaacta
     2701 tttttatatc cgatagttat aattataaca atgttttatt aattgatgat gttactggat
     2761 ccggagctac tttaaacgag acagctaaaa agttaataaa taatggtttg gctaaaaata
     2821 tttatggctt tacaataacc ggtagcgcca aggctaatga ttttgatgtt ataagtgaag
     2881 cttagcttaa taaaaaggtt aacaaccgtt ccaattacgc ccccctcgcc gtaaacacaa
     2941 taaagcatca gttaaacacc gatgcttttt cataagttaa taattacggg ttagattcac
     3001 ttagtttgcg ccccgtttac ccggtaaccg tgaaaaatta ggttaaatga cttattctac
     3061 ttttgcccat tgacatatat aaattttatt gttagtatct ttttattcgt tcatttgtca
     3121 acttattaaa cactttaaaa actaaatatc atgaaaattt tcagaattgt tctcattgtt
     3181 gtttctacct gttttatctt tttccctcgt ttacaggctc agaccgcagc ctttattatt
     3241 aaccgagaaa acaaagaaat tgtgctcctg ccaagatcgg gggggccggg cttaataata
     3301 ccggcaggag attctgtttg gtcgttcgat gtatcagata tatattatgt ctatcaacaa
     3361 attaaaaaat ctccacaagt ggtggagaag gttggttttt gtcgattgac ccctttagac
     3421 ggggcttatg aatttgctgg cgataaacta ccgatatttg taaaaaacaa ttccgagcag
     3481 aatgttttgt tggaattcag cgatatggtg gtttctctgc cggcgggtgg ctctttttgc
     3541 gccgatgatt tagtaatcga gcccgactta accgctttag tatatttaag cggtaacgac
     3601 tctgtttcgg cgccaggctt ccgtttattg tcttttacaa tcaacaacgg aagtttgtcc
     3661 gcaaacttat ggtaatggta aataaggaaa aatttttgag ccttcttttt agagggctct
     3721 tttttattgt ttttccctta attttttggt ataatgtgat taaagtttaa aaatatgttt
     3781 aattttttac acaaaaaaga atcaatttcg cctagcgacg aaaaagaccc tgaaaagaag
     3841 gatacaattg aagagccaaa ttcaactgaa ccgatattga ttgacaatga taataataaa
     3901 gaaaaggcca gcagtctcaa tcctgatagt ttagaaaaat taagggaagc tgttgagatc
     3961 caggaagaga tgggtaacca cttgtcttta gaagatactt tgcttgttac cggtacgagt
     4021 ccggaaaagc ttcccgaaat tttagccgag caagacataa atttagaggc taagggtgaa
     4081 caagttatac cagaaatcgc tagagtttta gagtccttag aagaagatga aaaatctgaa
     4141 ttaagcggat ttcgccgttt tactaattat aattcaatta aagccgcttt tattgcttta
     4201 atgcttctgg ttaaatttgc tccggacgcc caaggagcag agccacagga taagctgaaa
     4261 gagggagtga aaatgggtaa agaggtgaag ggcgagcttg atcgtgagcc caattcagat
     4321 aaaacgtatc agctgtcagc taaagatttt gatgacgagc ggccggaaaa acataagtct
     4381 aacgacgcta agcctttgga tataaaatta gaggaaaatt cacgtttggc tagtttggaa
     4441 ctaacacact attttgctac tgataaagct gatattatta acccccaagc gattatttcc
     4501 gattttgaag cttttttaag caagattact cctgacaatg ctcaagaaat tatggccgct
     4561 gattttaaat tatttggttc cagtgatgag cgcccgacga gcaattggaa gggcagcaat
     4621 actgagttga ctaaagcgag actaacggct ttagataaac tcctcaccga aactttacag
     4681 aattataagt ttgacaagtt gccggccgat ttagctgaac agattagaaa taagaagttt
     4741 tttcatgaga tgcctttttc ccagaccggg ccggaggagg gcgtgattta tattactgat
     4801 ttagataatc cagaaactgg tcaaaagtat acaccagaag aagtggcgtc tataaaagct
     4861 aattacccgg aaaaatataa gttgttgttg gacgattgtc gacggatatc attttcggca
     4921 actatgcccc agatggattt tatcaataag atgaaaatga ggaacagtga agttgaaaaa
     4981 gggtatgaag tcagtccaag gtctcccgaa tttcttaaaa tgaatgaata tgataatgtt
     5041 tatctgctgt ttgataatag cccgtctgta ggcgattcct atccttatat ggcgcagatg
     5101 atagaaaagc agaattttaa ggaactaaaa gttaattttg ccactttttc caatcatctg
     5161 gacggaataa agcagttaga caattcggtg gaggtggcca aaaatatcag agatacaaaa
     5221 tattttggtg atactagaga acgggcactt gatgtcgctt ataccgcttt gaataaaatg
     5281 aaggctgaag aaaaaaatgc cctttttatc atgaccgatg aggatattca ggctgtttct
     5341 tgggaaaaaa ttcaaaaagc ccgtagtctt gccgcggaga aaaatgcctg tgtctatttc
     5401 tattatggcg atgataagca tcaagcgatt agacaggttt ccttagacga gttagaaaga
     5461 gcttataagg agcaggcaat ggcggctatc gctcccaaga ttaatttttt atacaatgct
     5521 gccagcaaaa gaattagcgc tttagaatcc cggcgacaga gtcaagagaa tttgatttcc
     5581 cgtctagcgg cgaaaacgaa cattcctgct tatcaaaaga gcctcttggc tcaccagaaa
     5641 gaattgagca aaattaatga aaacctgaca aatgaaaagg aaaaattagc ggccttgatg
     5701 cgtgcttggg agggtggaga tattgaagac gtttttgttc aacaggcttt ttattggaaa
     5761 gttgctagtg atacagaagt tcagaaagtc cgtcaaggaa tagagattaa tgtagcggtg
     5821 aataatttag gttccgaagt ggttgattta gcgtctttat cagaagcaga cggccgttag
     5881 tgggtttgac tattttcaac aaatcaatta ttatcaagaa gtaaggccaa attacgccct
     5941 cggcgtaatt tttgttttaa aaagctaaaa atttcattat ttattttatg gaaatccgca
     6001 atatcgctat tattgcccac gttgatcacg gtaaaaccac tcttaccgat gctttgatgc
     6061 gccaaaccgg tatcgccggt gaagaagtaa gcatggacag caatgccttg gaaaaagaaa
     6121 gaggcattac tatttattca aaaaatactt cgatttttta taaagacaca aaaattaata
     6181 tcgttgatac gcccggccac gccgatttta gttcagaagt tgagcgtatt ttacgttcca
     6241 ttgattgcgt tttgctattg gttgacgctc aggaggggcc gatgccgcag acaaaattcg
     6301 ttttaaaaaa atctttagag ctcggtttca agccgattgt agtgattaat aagattgata
     6361 aaccggccgc tcgtccggcc gaagtggtgg aaagggtgta tgaattattt ttagattttg
     6421 gcgccaacga tgctcagctt gattttccgg taatctatgc cgctgccaaa cagggaatcg
     6481 ccaagaaaga attgaccgac gcctccgaca atctcaatcc tttgcttgat ttaatcataa
     6541 ctcatgtttc tctggcttct tccgaggagg ctttgtctag accgttattg gcccagcctt
     6601 ttaatttggc ttatgataat tttttggggc gctgcgccat ttgccgtctt tatgaaggaa
     6661 aaataaaagc agcgcaaact gtttttatta aagatcttga cggcaccacc agggccagaa
     6721 aaatcacgaa gctttttacc tttaaaggcg tcaatcgcca agaggtgccc gaagcggaag
     6781 cgggggatat tgtgatgatt gccggtttgc cggatattta tatcggcgag acgattgccg
     6841 aaagagccga ccaaaaagct ttgccagcga ttaaaatcga tgagccgaca atcgctttga
     6901 attttttggt caacaattct ccttttgccg gcagagaggg taaatttgtc accaacaggc
     6961 agttaaaaga acgcttggaa aaagaaatgg aagtgaacgt tggtttgaaa atcgattttt
     7021 ccgaacccag ttattatagg gttttcggcc gcggtgagct tcatatcgca attttattgg
     7081 aaaatctgcg tcgggagggt tatgagttgc aggtttctca gccgcaagtg attatcaaag
     7141 atattaatgg cgttaaatcc gagccttttg aagaagtgac gattgatatt ccggaaaatt
     7201 tatccggtat ggttattgaa aagatttcta aacgtcgtgg caaaatgctt gattttaaaa
     7261 cacacaaccg ccagagccgt ttgactttcg ccgtgccgac caggggttta ctcggttatc
     7321 gcaatgaatt tattgtcgat actaggggcg aaggcatcct ttgcgcccag gtgctgggtt
     7381 ttcagcctta tgccggcgat atttataaga ataatctggg ctcgatggtt tctatgatta
     7441 ccggcaaggc tttagctttc gctctgtgga atttgcagga tcggggcgtc ctttatatcg
     7501 cccccaatac cgaggtttat gaaggcatgg ttatcggtaa tgtttccaaa ggcaatgact
     7561 tggcggttaa cccgattaaa ggtaaacagc ttaccaatgt ccgtgcgtcc ggttcggatg
     7621 aagcgattca actgacacca ccggtgccct tgacgattga atttggcctg agcgttatga
     7681 aagaggatga atatttggag attacgccct taagccttcg tttgcgcaaa cagcatttga
     7741 ccgaagtaga gaggcaaagg gcgaagaggg taaaaatcta gggataatct aaaatatttt
     7801 tttaaaaaaa gctgtattta tacagctttt tgggannnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnttggt cttgatattt ttactgataa attacccgga tttgagcctt
     7981 atcatagcgg ctgttttcgt gatgacgata agaccagagt ggctgatttt aaaccggctc
     8041 ctcctggtga aataaaagat agtttaatag agttattagc ttggattaaa aaaaatcctt
     8101 cggcaattaa tgtttttatt tttcatgccg ccttgtattc tttacatcct tttaaaaacg
     8161 gcaataagcg ggtgtgtagg gttttggagc acttaatttt acgcgatatc gcttataatc
     8221 aaaataattt atacaacctc tcttattact attaccagca aaaagatagg tattataaaa
     8281 atttaattaa ctctttgtat aagcatagct ttaacccttt tgtggctttt gcttccgagg
     8341 ctttatattt ttccgccctg ggagtaatta agggagtatt ggagagaaaa aaacaagaat
     8401 ttattaacaa tagcaactta ggtttgcctg ctattaaatt attaaaacct ttggttaaaa
     8461 acggagagtt aaaattttct catttttacg ccttgtctaa aagaaaagtt tcgcggcaga
     8521 cttttattaa ttatttaaac gaggctttgg atcagcagat tattattaag agagaacagg
     8581 gcagaaatac ctattactcg gtggcgggca attatctgga ggtggatatt ttgaaagaaa
     8641 aattaaagat tgccagagaa aaactgaatt atttgccgcc ggatttaatc agttaccttt
     8701 agttgtctaa attctttaat tatttgtaaa aggacggaaa gctcggtatg taaaaagcca
     8761 tcccttaaag gatggctttt gcggagctga ttgcttacat tggtttttta gatattatta
     8821 agaattgttt taagattatc aataaatatt tctgtggtgt acaggcgatg ataacgctct
     8881 tggccggctt ggccaaattt tttccgtaat tttggggcgc tcaccagggt gttgatggct
     8941 ttattcaggg ccgctatatt tctaggtttg accgtgagtc cggtaacttc atttaggcta
     9001 acttcttcga ccccggtttg cagataagtg ttaattatcg gcttgccggc cgctagtgcc
     9061 tccagctgca ccagcccgaa agcttcgctg cgttcagttg aaggaaagag aaaaatatcg
     9121 gccgccagaa aaaaaggttt taaatctggt tggcaagaaa tgatgcttac atttttttgc
     9181 aagtttaaat ccgtaatcag tttttccagc ctgcctttct ccggtccgtc gccgataatt
     9241 agaagttgag ccttgactcc gctcatggct ttgatggcat atgcaaagcc tttataatag
     9301 acgaggcggc cgacggccaa taaaagaact ttgttttttc cgtattgtcg tttaacttct
     9361 tcagcttttt gataatcatt aggggtataa tctgcttgat aaccaaaagg taaaacctgg
     9421 catttatcgg aatgggtttt taaatttggc gaggagttta ttaaattatg gctacccact
     9481 aaaattgtgt gggctttttt gagactgtat ttgataaaag gagaaaagat taaggcgcct
     9541 aacttttgcc ggacaatatc agaatggtaa tagatgatta attttttttt aggaatgaaa
     9601 ggggaagtca gggctgccaa agggaagggg tagtgtaaga ttatttggtc gtagttattt
     9661 tttatatctt taaatagttg aaaaaaatct aaagataagg gcatgcctag ttttttaccc
     9721 caagaagcgg cgcgataaac cttaaccccc cgaacagttt ctatttgtct cttgcctttg
     9781 ttttggcaaa ctaaaacatc aaccgtaaac tcagattttt gattaagctt ttcggcgatg
     9841 ttctgaacaa ttgtttcaat cccgccgatg accggataat atagtttatt tatctgaaga
     9901 attttcataa agatgaagga tgtctactaa ttttttagca gatttttgcc aattgaaacg
     9961 agggtaattt ttactacctt cattttttaa attagccgct agcaccgggt tgttgataat
    10021 ctttttaatt ttttccgcta aatccgaggc cgataggggg tcaaagaata aagcgccagt
    10081 gttagccact tctttaaaaa cagggatatt agaacaaata atcggggtgc ccataatttg
    10141 cgcctccaag ataggcaggc cgaagccttc gtataaagaa gggaaaataa gggcttgagc
    10201 ttggcttaat aattgatatt tattttcatc actaataaat ccggtgaatt ctattctgtc
    10261 attctggtat gaattagcta aaatttgcat tctcttatcc atttttccaa caactattaa
    10321 acgtaaatca ggaatatcgg ttttaattaa gttataagca gccaagagag tggtgagatt
    10381 ttttcgcttg cgggcattgc caattacgat gatgtagggg ctgactgttt gtgtctgatt
    10441 tttattgata acgggtatta cgggagcgcc gttataaata acacttagtt tgttttgatt
    10501 aatttttaat ttattaatta attcttggcg agaaaattca gaaacggtta agaggtggcg
    10561 ggaaaagtgt ttcgcaaaaa acaaagatat cagccagtag atcttaccga agcggctgct
    10621 ttccccttta ataataaagg gtgcggcgtc gtggatagtg acaatttttt ggggatagag
    10681 ccaggggcaa tttaaagagg cgcaaaacag tatatccgtt tttctctttt taagtagacg
    10741 gccaagttta aattgttgaa aaataaaaat cgatagtttg tcgatttttt taaatttaat
    10801 tatttctaat ttgaagtaag ccggtaatgg ttgtagaaat ttagccgaaa tctcattagc
    10861 taagaccgtt atctcgtcct cattatgagg ccacgcccgc aacaaattag tcaggaaatt
    10921 ttcaattcct tgataagtat tataggggag ggcattgatg actattttca taagactt
//
LOCUS       DAOQSA010000025        10728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_5406_length_10728_cov_8.826665, whole genome shotgun sequence.
ACCESSION   DAOQSA010000025 DAOQSA010000000
VERSION     DAOQSA010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10728
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5406_length_10728_cov_8.826665"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..113)
                     /locus_tag="PKK65_03230"
     tRNA            complement(37..113)
                     /locus_tag="PKK65_03230"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(77..79),aa:Val,seq:cac)
     gene            complement(119..202)
                     /locus_tag="PKK65_03235"
     tRNA            complement(119..202)
                     /locus_tag="PKK65_03235"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(166..168),aa:Leu,seq:cag)
     gene            complement(313..825)
                     /locus_tag="PKK65_03240"
     CDS             complement(313..825)
                     /locus_tag="PKK65_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81723.1"
                     /translation="MKKVALLFLLTISASFLSAQAFYFNPLVSVGRIDTTGTRLVRQL
                     AIQITDSRNNNKFLLSGEVATGSRELKQHYAVFTHKGKRYSVYYQWDISEPILLMSCE
                     FLMISVHPEGTDDPEDLVSYKDYHLDGSWDEYSHGQYIYLAEYKDRFSDDNYQKNFLQ
                     TIKEALAFFQ"
     gene            942..1586
                     /locus_tag="PKK65_03245"
     CDS             942..1586
                     /locus_tag="PKK65_03245"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNU81724.1"
                     /translation="MLDLETEKQIFAAGHKLIGGVDEAGRGPLAGPVVAACVVIGPDF
                     KIDDEDLKLVTDSKKLSAKRREKLFGVIKNKALAVEIGVVNNKVIDKINILQSAFLAM
                     RQALDRLALQPDYVLVDGNLKIPQLKQKQSAIIDGDAKVFTIAAASIIAKVSRDWLML
                     EADKKYPEYEFARHKGYGTKRHLEKIKEFGPCPLHRLSFAPFAVKKDKNKKNAS"
     gene            complement(1613..2893)
                     /gene="hisS"
                     /locus_tag="PKK65_03250"
     CDS             complement(1613..2893)
                     /gene="hisS"
                     /locus_tag="PKK65_03250"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNU81725.1"
                     /translation="MKKVKLSNQEVKGTSDWLPAEFSVRHYIFETWRRVCSSYGFQEY
                     LTPLVESAEIYRVKSGEDVGGKELVTFTDLGGRELCIRPEMTPSVTRLVSKIYASSPK
                     PIKLFSIANFMRNEKPQRGRNREFWQLNCDIFGSASLAADLEILQLSLDIMKAFGAPE
                     NSFTLRLNSRRLLDNFLPIFGLDLKNKSIVFRILDKYGKLSQDEFIRRLVAVGAKEQS
                     AKSLEAFMAGTEKNLFLKALAENSDLRAAKQEIDFVLKQLQNLGYNQAQFDPTLIRGF
                     DYYDGLVFEVFDNHPDNRRALFGGGRYNGLAELFGTESFPAVGVAPGDETTRLFLESW
                     NLLPVIPDERYYLPLLSADLLSDTQRLAQILRAEGKNVLFAFEPQRPGKALEFANKQG
                     IDRVVIFGSNEKALGQYKIKEMKSGEEKIYYFKA"
     gene            3051..3728
                     /locus_tag="PKK65_03255"
     CDS             3051..3728
                     /locus_tag="PKK65_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNU81726.1"
                     /translation="MKILIIDDEKDIVAFLSRGLKSKAYIVDAAYDGEHGSLLARSNH
                     YDLIVLDGNLPKLNGLAVLKEIRSTGIKTPVLILTVEAEPEKKGEMFHQGADDYLTKP
                     FLFDEFLWRVQALLRRPQIIPEKSWRLGDLELDIKRQLALRRGRRIYLTGKEYALLEL
                     FFRRKDEVLSRSQIMDNVWENNADPFSNTIEAHIMSLRKKLNAAGETDYIHTFPGRGY
                     KFGLHRL"
     gene            complement(3730..4923)
                     /locus_tag="PKK65_03260"
     CDS             complement(3730..4923)
                     /locus_tag="PKK65_03260"
                     /inference="COORDINATES: protein motif:HMM:NF023428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNU81727.1"
                     /translation="MQYESMIAGLPLAAYLRPKKLEEVVGQTHILRSDSWLVQAIKND
                     RVSSLIFWGPPGSGKTTLAFLIAKETKADFVELSATESGVKDLKQIVEKARSGKRLGV
                     KTILFIDEIHRWNKSQQDALLPQVENGTIILIGATTENPSFALNGALLSRLKVVLLEA
                     LSEDDLSLIIKRAAKVLQVKIKVEAVRLAARLANGDARRAIGLIEEAASWQKIIDASL
                     IKDIVNKPNLLYDKSGEEHYNLISALHKSMRGNNPDASLYYLARMLEAGEDPVYIARR
                     LVRFASEDIGLANNSAAMLTEAVFAACKNIGMPECGVHLAHAVVYLAKSPKSIAVYAA
                     FNEAMAEVRNSGNLPVPLHLRNAPTKLMKDLGYGKNYKYTPLEDDSNQEYLPAALKGR
                     KFFKP"
     gene            complement(5236..8676)
                     /locus_tag="PKK65_03265"
     CDS             complement(5236..8676)
                     /locus_tag="PKK65_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNU81728.1"
                     /translation="MEINLHKPQEAGLAEETTIAKTSDHLYLKKLEFDPALRKSWLNF
                     FIVNFRVVILLIILLTALGLYSFFSLPRESSPEVKIPIAVVMTVYPGASPADVEELVT
                     KKIETKISGIKDVDKITSNSSNSVSAITVEFSSKADLNDSLRSLRDQVNSLKGTLPSD
                     VNDPIVKEISLDDTPIITISLAAPYDGFRLRSFGEDVRDELEKIAGVREVIISGGEET
                     EFEIAYDPQKLTFFNLSADAANQLVAAANLVIPAGTFEGREYSYPVRVDGRFFDATKL
                     GNTPLFHTDDGAIIYLKDVASVKETAIKKSVYSRLATRENGGQDNINLQIIKKTGGSI
                     LDISAEVQTILDRMTKALPGATYEITYNAADQINQDFNQLTHDFILTLLLVFTILFLI
                     VGFKEALVAGLAIPLVFFATFGVMLMTDISLNFLSLFSLILALGLLVDDAIVVVSATK
                     QYLRSGKFTPEEAVLLVLNDFKMVLTTTTLTTVWAFLPLLLATGIIGEFIKSIPITMS
                     VTLVTSLIIALIINHPLAAILERIRFTRKLFSFLFVGLIVISLGVWFLPLSIVWKLAL
                     SLPSLAAVIFLGRWYRHRGQKVLENNSVLVEQEWRDDDAIKKKLREQGSHDNNNFLNR
                     LFHGVIHFDRILPYYEKYLRRLLATKRSRLLTLAVTFFLFLVAVSLPATGIIKSEFFP
                     SYNSDVLYINLEAPSGLRLEETDKIVAQVEQRLFAYPEILNFTTLVGSSASFNGLGGG
                     NNSSHLASITIKLSKTDERKITSYQLADKIRADLSDIKAAKITVESLAGGPPFGAAFE
                     AQIKGDDLQVLDKIANDLEVYLRSIPEVVSTDISLKESPADYTFTLDPARLELYSLNA
                     AYVGSTLRMAISGTEVTTVLRDGKEIKVIARFAKDKIPTLESIQNLQIINLKKQPVFL
                     KDVAKIELKPSVETITRIDQKRTVILNAGLKGGANSTLVLKEFQEKIKDYQFPNNYEI
                     SYGGENEQNQESVLSIIRAMAVAFVLIISTLVIQFNSFKKAAIVLTTIPLALIGVFFG
                     LALAGINLSFPGLIGILALFGIVVKNAIILVDKINLNLRSGIPFKDAIVDAGKSRLEA
                     IFITSICTIFGILPITLSNEMWQALGGSVIFGLMLSSFMTLFIVPTLFMTFIKDKGRY
                     "
     gene            complement(8681..>10728)
                     /locus_tag="PKK65_03270"
     CDS             complement(8681..>10728)
                     /locus_tag="PKK65_03270"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNU81729.1"
                     /translation="TRRLFKKHNLRIENYFSSKEMENFLRLIRKLNQGLDKCEININK
                     NNKYEKNLSLLAVLVLSLLFSGCGTSQPVAEPTPSAIKVKTQSLADSKEIRQDLEYPA
                     IVSASTEAKLVAKSSGNLSGADFKVGDEVKIGQTLARIDDIGSSGFSTDGINSNQIKQ
                     AIIAVEQAQSFYQLARSNYENLLVSSAKDLHQAEIARDQAKNAQSHLNTTSEESLKSA
                     ELAYETAKIATEQARLSLLNRQKQMQQGSLDANDNADLAATAAVNTAGNVLTGINNLA
                     AFDDNNVVSIAYRGNLGALDSNSYNQADNDYQAAKSAYDVYQTTSFSDISERLQAAID
                     LAEKVKKLADSSKYLFDKSIPSSVLPQSAATGVSLSGLQSAAASYQSQLNAVIGQARG
                     AAQALANVALSNDSTLDALKQAYELAKKQEAIAQQNLNNLKAGNNSQSDQAGFSVSLA
                     QNQYENLKVKLDSQILAAKAQMDAAQLQYNNALVALQSLYDIHSIVSPIVGRVTQKLA
                     NNGDTVSAGQVVAVVSQVDNLKVRFFVEPEYVLDMKLGTPVEVLSADGQSYAGLISSV
                     AAQADNATKRFQVEVKLDDQAKPLLGTVVSVKLSLEKSVASGDGEAIVPLSSLEIGQN
                     GNFIFLFEDGKARKVPVELQKVLGERAQIKVEAPADAILIIEGNKLLRDGDVVEASA"
BASE COUNT         3402 a         2341 c         2085 g         2900 t
ORIGIN      
        1 cgcccgagcg agacggaaaa ctcatcaaat cgtttatggt ggtcgttggt ggactcgaac
       61 cactgacctc tgcgatgtga acgcagcgct ctaaccagct gagctaaacg accaaacgtg
      121 cccgaagggg gactcgaacc cccaccccct tgcgaagact agcacctgaa gctagcgcgt
      181 ctaccaattc cgccatccgg gcgatggaat catgcctata gcttgccaca ggaagggcta
      241 tttgtaaaga ctaactaata aaaaaccgcc attcccataa atagaaacgg cggaaaaata
      301 ttagattgca ccttattgaa aaaaagcaag ggcctctttt atggtttgaa gaaaattttt
      361 ctgataatta tcatcagaaa atctatcttt atattcagcc aagtaaatat actgaccgtg
      421 agaatactca tcccaactac catcaagatg ataatccttg taggacacaa gatcctcagg
      481 atcgtccgtg ccttcgggat gaacagaaat cattaagaat tcacagctca tcagcaagat
      541 gggctcactg atatcccatt ggtagtaaac gctgtagcgt ttgcctttat gggtaaacac
      601 ggcatagtgc tgtttcaatt cccggctgcc agtggctacc tccccggaga gtagaaactt
      661 gttattattt ctactgtccg taatttggat agctaactga cggaccaatc tggtgccagt
      721 agtgtcaatg cgaccgactg aaaccagggg gttaaaatag aatgcctggg cgcttagaaa
      781 acttgcagaa atggtcaaca agaacaacaa agctactttt ttcatcttat aataaatttt
      841 agggtttaaa tgtacgtgaa ttaacaattt aatgtatcat aattaaatat ttatgtcaat
      901 aaatagttaa aaaaacaaag aaaatgctat aattaaaaaa tatgcttgat ttagaaactg
      961 aaaaacaaat atttgctgcc ggacacaaac taatcggtgg cgttgatgaa gccggtcgag
     1021 gacccttagc cggaccggta gtggccgctt gtgttgttat cgggccggat tttaaaattg
     1081 atgatgaaga cttaaaatta gtgaccgact ccaaaaaact gagcgccaaa cgccgggaaa
     1141 aattatttgg cgttattaag aataaggcgc tagcggtaga aatcggggtg gtaaacaata
     1201 aagtgataga taaaattaat atcctccaaa gcgcttttct ggcaatgcgc caggcgcttg
     1261 atcgcctagc ccttcaacct gattatgtct tagtggatgg caatctcaaa ataccacagt
     1321 taaaacaaaa acaaagcgct attattgatg gcgacgccaa agtttttacc atcgccgccg
     1381 cctctattat tgccaaggtt agtcgcgact ggctgatgct ggaagcggat aaaaaatatc
     1441 ctgaatatga atttgctcgc cataaaggtt acggcactaa aagacatctg gaaaaaatta
     1501 aagaattcgg accctgtccc ctccatcgtc tcagctttgc cccctttgct gtgaaaaaag
     1561 acaaaaataa aaaaaacgcc tcctgaaagg gggcgctttt taaaaagata aattaagcct
     1621 taaaataata tattttttct tccccgcttt tcatctcctt aatcttatat tgacctaggg
     1681 ccttttcatt gctcccaaaa ataaccaccc tgtcaattcc ctgcttatta gcgaattcca
     1741 aagccttacc aggacgttga ggctcgaaag caaataaaac gtttttacct tctgcccgta
     1801 aaatttgagc taagcgctga gtgtcagata gcaaatccgc actcaacaaa ggcagataat
     1861 aacgttcatc cggaataact ggcagtaggt tccaactttc taaaaacaaa cgggtagtct
     1921 catcgccggg agcgacgcca actgccggga aactctccgt accgaaaagt tcagctagac
     1981 cgttatagcg tccgccgccg aataaagctc ttctattgtc aggatgatta tcaaaaactt
     2041 cgaaaactag gccgtcataa taatcaaaac cgcgaattaa agtcgggtca aattgagcct
     2101 ggttataacc taaattttgt aattgtttta aaacaaaatc aatttcttgt ttagccgctc
     2161 gcaaatcaga attttctgcc aaggctttta aaaataaatt tttttcagtt cccgccataa
     2221 aagcttctaa actcttagcc gactgctcct tggcccccac tgccactagg cggcggataa
     2281 attcatcctg gctgagtttt ccgtatttat ccaaaatcct aaaaactata gatttatttt
     2341 ttaaatcaag tccaaaaatc ggtaaaaaat tatctaaaag gcgacgacta ttaagacgta
     2401 aggtgaagga attttccgga gcgccgaaag ctttcatgat atcaagggat aattgtaaaa
     2461 tttccaaatc ggccgccaaa gaagcgctgc caaaaatatc gcaattaagc tgccaaaatt
     2521 cccgattgcg tccgcgctga ggcttttcat tgcgcataaa atttgcgatg gaaaataatt
     2581 ttatgggctt gggcgaagag gcataaattt tggaaaccaa gcgcgtcaca gaaggggtca
     2641 tctcgggacg aatacataat tcccgaccac ccaaatcggt aaaagtcacc aattctttac
     2701 cgccaacatc ttctccggat ttgacgcgat atatttctgc cgattcaacc aatggcgtca
     2761 gatattcttg aaagccgtaa cttgaacaaa cgcggcgcca agtttcaaaa atataatggc
     2821 gtacagaaaa ttcagccggc agccagtcac tggttccttt tacttcctga ttactcaatt
     2881 taactttctt cataaaattt atatttaaag tctacttcta ttataaagga ggcaggaatc
     2941 attgtccatt atctcgttta aagataagtg ataaaaaatt taaaaagaat ttatccgtta
     3001 aatattataa ttataaggct ccactttaga gagcataaaa ttataaatat atgaaaattc
     3061 ttatcattga tgacgaaaaa gatatcgttg ccttcttaag ccgcggcctt aaaagtaaag
     3121 cttatatcgt cgacgccgcc tatgacggcg aacatggttc tttactggca cgcagtaacc
     3181 attatgatct gattgttctc gacggtaact tgcccaaatt aaatggcctt gccgttctta
     3241 aagaaattag atcgacaggc attaaaacac ctgtactgat attgaccgtt gaggccgaac
     3301 ccgaaaaaaa gggagaaatg ttccatcaag gagctgatga ttatctgact aagcctttcc
     3361 tatttgatga atttctgtgg cgggttcaag ctttactgcg tcgcccgcaa atcataccag
     3421 aaaaatcttg gcgcttgggc gatttggaac tggatatcaa aagacagtta gctttaagaa
     3481 gggggaggcg catctattta accggcaaag aatatgctct tttagaacta ttcttccggc
     3541 gtaaagatga agttctctct cgcagccaaa ttatggataa tgtctgggaa aataatgccg
     3601 atcctttctc taatactatc gaagcccata ttatgagcct acgcaaaaaa ttaaacgccg
     3661 cgggagaaac tgattatatc catactttcc ccggaagagg ttataaattc gggttgcacc
     3721 gtctataaat tatggcttaa aaaattttct gcccttcagg gcggcgggca gatattcctg
     3781 attagaatca tcttctaaag gagtatattt ataattttta ccgtagccca aatccttcat
     3841 caacttggtg ggcgcattgc gcagatgtaa gggtaccggc aaattgccac tatttcttac
     3901 ttctgccatg gcttcattga aagccgcata aacggcaatg cttttaggag attttgccaa
     3961 ataaactacg gcgtgggcga gatggacacc acactctggc ataccgatat tcttgcaggc
     4021 cgcgaaaacc gcttctgtga gcatggccgc actattattt gccagaccga tatcctcact
     4081 ggcgaaacgc accagacgcc gagcgatata tacggggtcc tcacccgcct ccagcattcg
     4141 cgctaaataa tataaagacg catcaggatt attaccgcgc attgatttat gtaaagcgga
     4201 tataagatta tagtgctctt cgccactctt atcataaagt aaattcggct tattaacgat
     4261 atctttaata aggctggcat ctataatctt ttgccaagag gcggcctctt caattagacc
     4321 aatagcgcgg cgagcgtcac cgttagctaa gcgagcggct aagcgaacgg cttcaacttt
     4381 tattttaacc tgtaaaactt tggccgccct ctttataatc aacgaaagat cgtcttcgga
     4441 caaggcctca agcagcacca ctttcaaacg cgagagcaaa gcgccgttaa gggcaaaact
     4501 gggattttca gtagtggcgc cgattaaaat aatcgtgccg ttctctactt gcggcagtag
     4561 cgcgtcttgc tgggatttat tccaacgatg aatttcatca ataaataaaa ttgtctttac
     4621 tcccaatctt ttaccggaac gcgccttttc gacaatctgc tttaaatcct taaccccgct
     4681 ttccgtagcc gacaattcca caaaatccgc cttagtttct ttagcaatta aaaaagctag
     4741 ggtggttttg ccggaaccgg gaggacccca gaagataagc gaagaaacac gatcattttt
     4801 aatcgcttgc actagccagc tatcactgcg caaaatatgc gtctgaccga ctacttcttc
     4861 tagttttttc ggccgcaaat aggcagccaa gggcaaacca gcaatcatac tctcatattg
     4921 tatactgatt catattttcc gaccattctt gacaaagttt atatattata gtgtaataaa
     4981 aataatctta aaaattcaag ccatgagaac aataatagaa ttttttcgac tattcttatg
     5041 ggctcatgcc ctaagaaaaa aagcagcaaa aagagaaaaa aggagatgcc aggctacaaa
     5101 aaaatggact ctatttaatc gctcgcgctc acactcacaa gactctagac ctgtatttat
     5161 gtaattatta aataaaaaca gggcaacaaa aaaggcgata ctgactaagc atcgcctttt
     5221 tctataacat aatttttaat aacgcccctt gtctttaata aatgtcataa aaagagtggg
     5281 gacgataaac aaggtcataa aagaagagag cattaaaccg aaaataactg atccgcccaa
     5341 agcttgccac atttcattag ataaagtaat tggcaggatt ccaaaaatcg tacaaataga
     5401 agtgataaaa attgcctcca aacgcgattt gccagcgtcg acgattgcat ccttaaaagg
     5461 aatgccgctt cttaagttta aatttatttt atcaaccaaa ataattgcat tttttaccac
     5521 aatcccaaat aaagccaaaa taccaataag accggggaag gaaagattaa taccggcaag
     5581 cgccaatccg aaaaatacgc cgataagagc gagggggata gtagtcagaa caatcgctgc
     5641 tttcttaaaa gaattaaact ggataaccaa agtagaaata atcaagacaa aagccactgc
     5701 cattgcgcgg ataattgata gcactgattc ctggttctgc tcattttcgc cgccataaga
     5761 aatttcataa ttattcggga attgataatc tttaattttc tcttgaaatt cttttaaaac
     5821 taaagttgaa ttcgctccac ctttaaggcc ggcatttaaa ataaccgttc gcttctggtc
     5881 gattctggtg atggtttcca ccgaaggctt taattctatt ttggcaacgt ctttaagaaa
     5941 gaccggctgc tttttcaaat tgattatctg taaattctgg atcgactcca gtgttggaat
     6001 cttgtcttta gcaaaacgtg caattacttt tatttctttg ccgtccctta aaacagttgt
     6061 cacttcggta ccggaaattg ccatgcgcaa agtcgaaccg acataggcgg cattcagact
     6121 gtaaagttcc aaacgagcag gatcaagagt aaaagtgtaa tcagccggtg actctttcaa
     6181 ggaaatatca gtacttacca cctcagggat tgaacgcaaa taaacttcta aatcattggc
     6241 aatcttatcc aagacctgca aatcatcacc ttttatttgc gcttcaaaag cggcgccgaa
     6301 aggcggtccg cccgctagag attccactgt aattttagct gctttaatat cgctcaaatc
     6361 agccctaatt ttatctgcca actgataaga agtaattttg cgctcatcag ttttactcag
     6421 cttaatcgta atcgaggcta agtgtgaaga gttattccct ccccctagac cgttgaaaga
     6481 ggcggaagat ccaactagag tggtaaaatt taaaatttcc gggtaagcaa acaaacgctg
     6541 ttcgacttgc gcgacaatct tatcggtttc ttctaaacgt aaaccagaag gcgcctctaa
     6601 attaatatac aaaacatcgg aattgtaact gggaaaaaac tcactcttaa tgatgccggt
     6661 ggcaggtaag gaaacggcga ctaaaaatag gaaaaatgta accgccaaag taagcaaacg
     6721 actgcgttta gttgctaaaa gccggcgcag atatttttca taataaggca gaatgcggtc
     6781 aaaatggata acaccatgaa acaagcgatt taaaaaatta ttattatcat gacttccctg
     6841 ctcacgtaat tttttcttga tagcatcatc atctcgccat tcctgttcta ctaggacact
     6901 attattctct aatacttttt ggccacggtg gcgataccag cgtcccaaaa aaataaccgc
     6961 tgccaaagaa ggtagagata gggctaattt ccaaacaata gataaaggca aaaaccaaac
     7021 gcctaaagaa attacaatca aaccgacaaa taaaaaggag aataatttgc gcgtaaaacg
     7081 gatgcgctcc aaaatagcgg ctaaaggatg gttgataatt aaagcaataa tcaaagaagt
     7141 gactaaagta acagacatgg taatgggaat tgatttgata aattcaccga taatgccggt
     7201 ggcgagcaaa agcggcaaaa atgcccagac cgtagtcaaa gtggtggtgg ttaaaaccat
     7261 cttaaaatca ttcaatacca ataaaacagc ttcttctggg gtaaacttgc cagaacgaag
     7321 atattgcttg gtggcggaaa cgacaacgat agcatcatca accaataagc ctaaagctaa
     7381 aatcaacgaa aataaagaca agaaattaag actaatgtcg gtcatcaaca tcacaccgaa
     7441 agtcgcaaaa aataccaacg gaatagctaa tcccgccact aaagcttcct taaatccaac
     7501 tattaagaaa agaatcgtga aaaccaacaa tagagtcaaa ataaaatcat gagttaattg
     7561 attaaaatct tgattaatct gatcggcggc attataagta atttcataag tagcccctgg
     7621 aagcgctttg gtcatccggt caaggattgt ctgtacctcc gcggaaatat ccagaatgga
     7681 gcctccggtc tttttgataa tctgcagatt gatattatct tgaccgccat tttccctagt
     7741 agctaaacga gaatatacgc tctttttaat ggctgtttct ttgaccgaag caacatcttt
     7801 taaataaata atagcgccgt catcggtatg aaataaagga gtgttgccaa gcttggtagc
     7861 atcaaaaaaa cgtccgtcta cgcggactgg atatgaatac tctcggcctt caaaagtgcc
     7921 ggccggaatt actagattag cagccgccac cagttgatta gcggcgtcgg ctgataaatt
     7981 aaaaaatgtt agtttttggg gatcataagc aatctcaaat tcagtttcct cgcctccgga
     8041 aataatcact tcgcgcaccc ccgcaatctt ttctaattca tcgcgcacat cttcgccaaa
     8101 actgcgcaat ctgaaaccgt cataaggagc ggctagtgat atagtaatga taggagtgtc
     8161 atccaaagaa atttctttaa ctatcggatc attaacgtcg ctaggcaggg ttcctttgag
     8221 actattgacc tgatcacgca gactgcgtaa agaatcatta agatcggcct tggaactaaa
     8281 ttcaaccgta atcgccgaga cagaattgga ggaattagaa gtaattttat cgacatcctt
     8341 gattccagaa atcttggttt caattttttt ggtaactaac tcctcaacat ccgccggaga
     8401 ggcgccggga taaactgtca taacaacagc aatcggaatt tttacttccg gactggattc
     8461 acgcggtaaa gaaaagaaag aataaagtcc taaagcggtt aagagaataa taagtaaaat
     8521 gacaacccga aaattaacaa taaaaaaatt gagccaactc ttgcggagag ccgggtcgaa
     8581 ctccaacttt tttaaataaa ggtgatcgga agttttagca atagtcgttt cctccgccag
     8641 gccagcttct tggggtttat gtaaattaat ttccatatat ttaagcactc gcctcaacga
     8701 catcgccgtc gcgcagtaat ttattaccct caataatcaa aatagcgtca gccggcgctt
     8761 ctacttttat ttgcgccctt tctcctaaaa ctttctgtag ttccaccggc actttacgcg
     8821 ctttcccgtc ctcaaataaa aagataaaat taccgttctg acctatttcc aaggaagaca
     8881 aaggcacgat agcctcaccg tcaccgctgg cgacgctttt ttctaaagac aatttgacgc
     8941 tcaccactgt gcctaataaa ggcttagcct gatcatccaa cttgacctcc acctggaaac
     9001 gcttggtggc attgtcggct tgagcagcga cggaagaaat taagccggca tatgactgcc
     9061 cgtcagcaga taaaacttct accggagtgc ccaatttcat atctaggaca tattccggtt
     9121 caacaaaaaa tctcacttta agattatcaa cttgactgac aactgctacc acttgtcccg
     9181 cgctaacggt atcgccgtta ttagccaatt tttgagtaac tctaccaaca attggcgaaa
     9241 ctatggaatg aatatcatat agactctgca aagcgaccaa ggcattatta tactgcaact
     9301 gggccgcatc catctgtgct ttagccgcta atatctggga atcaagctta acttttaaat
     9361 tctcatattg attctgagca aggctgacag aaaatcccgc ctgatcgctc tgactattat
     9421 taccggcttt gagattattc aagttctgtt gagcaatggc ttcctgcttc ttagctaatt
     9481 cataggcttg cttgagggca tccagagtgc tgtcgttgct taaagcgacg ttagctaaag
     9541 cttgagctgc tcctcgagct tggccgatga cggcgttaag ctgagattga taagaggcag
     9601 ctgccgattg caaaccggaa agagaaacgc cagtcgcggc tgattgcggc aaaacagagg
     9661 aagggatact cttatcaaat aaatacttac tgctatcagc taactttttt accttttccg
     9721 ctaaatctat agctgcttgc aagcgctcag aaatatccga aaaactagtc gtctgataaa
     9781 catcgtaagc gcttttagca gcttgataat cattgtcggc ctgattatag gaattggaat
     9841 caagagcccc gagattaccg cgataggcaa tagagaccac gttattatca tcaaaagcgg
     9901 ctaaattatt aatcccggta agaacattgc cggccgtatt aacagccgcg gtagcagcta
     9961 agtcggcatt atcattagca tcaagacttc cctgttgcat ctgcttctgg cgattaagca
    10021 aggataagcg cgcctgctca gtagcaatct tggcggtttc ataggccaat tcagcgctct
    10081 ttagactttc ttctgaagtc gtatttaaat gagattgagc attttttgcc tgatcgcgag
    10141 cgatttctgc ctgatgcaag tctttagccg aagacacaag caaattttcg taattagagc
    10201 gcgccaattg ataaaatgat tgcgcctgct ccacggcaat aatggcttgt tttatctgat
    10261 tgctattgat accgtcagta ctgaatccgg acgaaccaat atcatcgata cgagccagag
    10321 tctggccaat tttcacttca tcgccaacct taaaatcggc tccagaaaga ttaccagaac
    10381 tcttcgctac tagtttcgcc tcagtactgg cggaaacaat cgccggatat tctaaatctt
    10441 gccttatttc cttgctgtca gccaaactct gtgttttcac ctttatcgct gacggcgtcg
    10501 gttccgccac tggttgggaa gtaccacaac cagaaaaaag aagggataaa actaacactg
    10561 ctaataaact aagatttttt tcatatttat tattcttatt gatattgatt tcacatttat
    10621 ccaaaccctg attaagcttg cggattaaac gtaagaaatt ttccatctcc ttagagctaa
    10681 aataattttc aatacgaaga ttatgtttct taaaaaggcg acgcgtgg
//
LOCUS       DAOQSA010000026         9458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_6653_length_9458_cov_8.766032, whole genome shotgun sequence.
ACCESSION   DAOQSA010000026 DAOQSA010000000
VERSION     DAOQSA010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9458
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6653_length_9458_cov_8.766032"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2092)
                     /gene="ppsA"
                     /locus_tag="PKK65_03275"
     CDS             complement(<1..2092)
                     /gene="ppsA"
                     /locus_tag="PKK65_03275"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HNU81730.1"
                     /translation="MIKPVFVRFFEELSIKDVPSVGGKNASLGEMYRNLKDKGIKVPN
                     GFATTADAYHYFLESTGLKKKIKEILKDLDTKDTRNLMAHGKAVREAILSVQLPADFK
                     KEIADAYKRLSAENKSKALNVAVRSSATAEDLPDASFAGQQETFLNIVGEKELLLAVK
                     KCIASLFTDRAISYRADKGFDHFSIALSVGVQKMIRSDLGASGVMFTIDTESGFRNTV
                     LINSIYGLGENIVQGRVNPDEFYVFKPTLAIISRSIGKKTVKMIYNKGGKSPVKNIAV
                     SASDRLKPSISDAQVKKLAKWGIEIEEHYGRAMDIEWAIDGRDQSLYIIQARPETVQS
                     LRSLSVIEEYRLQKKGTVLVKGQSVGNKIGAGKTRRIMDVKQIHSFKPGEVLVTDMTD
                     PDWEPIMKIASAIVTDKGGRTCHAAIVSRELGIPCVVGTNNGSKVLNSGQSVTVSCAE
                     GEEGFVYAGKLPFKVLSTDIKNLKRPKTKIMMNIGEPELAFAQSFVPNDGVGLARLEF
                     IINNYIKIHPLALINYPHLQDKEAQKKIKQLTAGYEDKSQYFVDRLAQGIAIIAAAFY
                     PKEVIVRLSDFKTNEYANLIGGREFEPVESNPMIGWRGASRYYAKQFVPAFNLECKAI
                     KRVRDDYGLTNLKLMVPFCRTVEEGKKVQEIMAKNGLKRGVNGLQIYVMAEIPSNIIL
                     ADKFCDIFDGFSIGS"
     gene            2193..2274
                     /locus_tag="PKK65_03280"
     tRNA            2193..2274
                     /locus_tag="PKK65_03280"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2227..2229,aa:Leu,seq:tag)
     gene            2284..3105
                     /gene="lgt"
                     /locus_tag="PKK65_03285"
     CDS             2284..3105
                     /gene="lgt"
                     /locus_tag="PKK65_03285"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNU81731.1"
                     /translation="MFYFLHSFLPNSILFSFGPLTVHWYGLFLVLGMAAGITVAWKLS
                     SFYGIEKEKILDLAIWLIVSGLLGARIYELFLEFPYYYQNPTAAFKFWEGGLAIHGAI
                     IGGAIALFLFAKRYHLSAARLGALSVTGLSLGQALGRWGNWFNQELFGLPTNLPWGIP
                     IAEINRPLQYINETFFQPTFLYESLGCLLIFTFLLFLNYKWKNKIDDKKAIIIIIFYL
                     IAYSLLRFSLEFIKVDITPMLWGWRWPQIMSLIIITTSLIYLFINYHPFKHKTKN"
     gene            3107..4288
                     /locus_tag="PKK65_03290"
     CDS             3107..4288
                     /locus_tag="PKK65_03290"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNU81732.1"
                     /translation="MLLKIFNRVNKRKNLVLLYLLGLILAIANALPAYIQSNFLGQIF
                     SLSWVSIFFASANLITVFAIIFFPKLIKKIGNIASTEIILFLFIFSLLGMSLANHAIV
                     IFIAFILLSVTSNLIWINMDILVESFSDDTSTGLTRTIYFTAINLGWIIAPSLSSYLI
                     EIGDYYWVFIAAAICLVPFMAILIKNRHRLRDRGRYRTHPILPTMKKLWKKPNLRSIF
                     FIALLLNLFFSSAVVYIPVYLHQNLGFDWSVLGIMFSIMLVPFIIFEIPAGWLADKYW
                     GEKEIMGTGLAIIIIALFLFFVVREADPWLWGGLLFFSRVGASLVEAMRESYFFKIVS
                     AKDVQYINFFRTTAPLGYLLGTILAIIIIYFYPLQYLFLFIALIMTSGFLFLYTIKDS
                     K"
     gene            4338..4409
                     /locus_tag="PKK65_03295"
     tRNA            4338..4409
                     /locus_tag="PKK65_03295"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4371..4373,aa:Gly,seq:tcc)
     gene            complement(4438..5139)
                     /locus_tag="PKK65_03300"
     CDS             complement(4438..5139)
                     /locus_tag="PKK65_03300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNU81733.1"
                     /translation="MLSIVIPTLNEEKYLPRLIASLKKQNFSDYEIIVSDGMSNDGTK
                     EAALSASCVFIQDEKRSPARQRNRGAAVARGDVILFLDADTMVPDNFLPAIYNEFKSR
                     HLSVAGFYLIFNSPRHIYRLFEFFYHFICFIGQFFFPASVGVGIMVDKKAHDYQNGFD
                     ESIFIGEDYDYIKRLSKHGRYRMLKSAYLYFSVRRLEKEGVSSVLWKWFKGGLYFLIR
                     GPIRKKIVDYDFGKY"
     gene            complement(5141..6139)
                     /locus_tag="PKK65_03305"
     CDS             complement(5141..6139)
                     /locus_tag="PKK65_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNU81734.1"
                     /translation="MKIKRKIVYLIIIVFLVTTVLFIKIFQKEQDIEIKSNPEFFGVT
                     FSKKYATEIGLNWRELYFAILDDLQVKNIRLPIYWDEIESEKGNFNYSDYDFMISEGS
                     KRNVRFIINIGWRLPRWPECHAPEWTNQEDLAHVQERALLMLEKTVRRYQDNPSILYW
                     QVENEPFLNTFGICPPSDEAFFAKEVALVKSLDKRPIMVSGPGELNMWRKEAKYGDVF
                     GTTMYRVIWNKVIGYMRYPIPAWFYRFKAWLVNIPADKRLIVELQAEPWVPQGKIIYL
                     SKTEADYSFSLDQFKANLQYALDTKFKQAYLWGVEWWYWQYKHGDKSFWDLARTLF"
     gene            complement(6136..7077)
                     /locus_tag="PKK65_03310"
     CDS             complement(6136..7077)
                     /locus_tag="PKK65_03310"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNU81735.1"
                     /translation="MTWFFVALSAYFLLAITNLVDKFLVEKVVGSARAYTFIACAMGS
                     LIFLVAPWFLSWPGTFFFWFNLFLGLLFALALLFLYASLRRGEASRSLVIVGGSTPIF
                     SLPLSFFLLGDKFSTNQLIAISLLLLGLLVMVFLPEKRQNFWEKVFTSLNLKHSATLL
                     SVFLALLSGLFYALFFVGSKFAYASQDFFSAFLWTRLGALLAASLILFSPRARREIKA
                     IFTRQPKRKRNQGLIFGNQVLGAIGFVLQNYAVYLGPVAIVNSLQGVQYAWIIFLGAI
                     ISFFYPKALKEDISKRVLFQKGLAIFIISIGLYFLSL"
     gene            complement(7081..8358)
                     /locus_tag="PKK65_03315"
     CDS             complement(7081..8358)
                     /locus_tag="PKK65_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81736.1"
                     /translation="MDNKKIYLVAADMGYGHQRAAYPLLSLSRNGIINLNDYPEIDNW
                     EKSYWLNSQKSYEKISYFKRVPVLGELVFRTMDYFQKIDAFYPRRDLSKSTLQQKLFF
                     QAVKKGVGKKLIESLNKEVLPLVTTFFVAAYCAEYYNYQGLIYCVVCDADISRAWAPI
                     NPQSSRIIYLCPNERVGERLKEYGVSSDKIRITGFPLPIDNLGPDDSLLKNDMAIRLG
                     GLDPRGVFCHKYKSLLISLFPEIQPAYRPLELTFAVGGAGAQREIGALILRRLEASIK
                     SGKIKFNLVAGSRADVNEYFKKAVLNLGLNDNDNVRIIYHPQKMEYFRLFNLCLRKTD
                     ILWTKPSELSFYTGLGLPIIMSEPVGAQEKFNREWLLSIGAAFDEINPEFVDEWLFDW
                     LDDGRLARASLNAYLHAGHQGVKNIEKEIFSNF"
     gene            complement(8443..9375)
                     /locus_tag="PKK65_03320"
     CDS             complement(8443..9375)
                     /locus_tag="PKK65_03320"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNU81737.1"
                     /translation="MYDTIIIGQGPAGITAGIYAARREMKTLILGKEPGGQIIWAGKI
                     ENYPGFTSINSFELISRFNEHALASGVEIKNAEVKRVEKQTDGSFLLFTNRDKYEAKT
                     VIIAMGLSPRRLAVPGEVEFNGKGVSYCANCDGPLYKGKTVAVIGSGNSAFDAAEVLS
                     KIAAKVYLITRGATFKAFDSLVDEVKNRDNIEIITGAQTREIKGGDKVESLVYQNDSG
                     QDISLSLDGVFIEIGRIASTNLVADLVDRDENLQILVDNKYATRTPGLFAAGDVVASD
                     FKQITIAMGQATSAALYAYQYIQGAGASLITTSK"
BASE COUNT         3071 a         1998 c         1543 g         2846 t
ORIGIN      
        1 tggaaccgat agaaaagccg tcaaaaatgt cacagaactt atcggctaaa ataatattac
       61 tcggaatttc cgccatcaca tagatctgca aaccgttaac gccgcgcttt aagccgttct
      121 tagccatgat ttcctgcacc tttttacctt cttccacggt tcggcagaaa ggcaccatta
      181 acttcaaatt agttaggccg taatcgtcgc gaacgcgctt tatcgcctta cattccaaat
      241 taaaagccgg tacgaactgc ttagcataat agcggctagc gccgcgccaa ccaatcatcg
      301 gattagactc caccggttca aattcgcgac cgccgattaa attagcatat tcattggttt
      361 taaaatcaga caaacgcacg attacctctt taggatagaa agcggcggca ataatggcta
      421 tgccttgagc cagacgatca acaaaatatt gagatttatc ctcatatcca gcagttagtt
      481 gtttaatttt cttttgagct tccttgtctt gaagatgagg ataattaatt agggctaaag
      541 gatgaatctt gatataatta ttaatgataa attccaaacg ggctaaacca acgccgtcat
      601 taggcacgaa agattgagca aaagccaatt ctggctcgcc aatattcatc attattttcg
      661 ttttggggcg ctttaaattt ttaatatcag tgcttaaaac tttaaacggc aatttgccgg
      721 cataaacaaa accctcttca ccctcggcac agctgacagt tacgctttgt ccgctattta
      781 agactttaga accattatta gtaccaacca cacaaggaat accgagttca cgggaaacaa
      841 tggctgcatg acaagtacga ccccccttat cagtgacaat agcggaagcg attttcataa
      901 tcggttccca atcaggatcg gtcatatcag tcaccaaaac ttctcccggc ttaaagctgt
      961 gaatctgctt cacgtccata attcgcctgg tcttaccggc accaatctta ttgccgacac
     1021 tctgaccttt taccaaaacc gtccctttct tttgtaaacg atactcttca ataacactaa
     1081 gactacgaag gctttgcacc gtttctggac gagcctgaat tatataaaga ctttgatcgc
     1141 gaccgtcaat cgcccattca atatccatcg cccggccgta atgttcctct atttctatcc
     1201 cccattttgc caattttttg acctgagcgt cgcttataga cggtttcaga cgatcagaag
     1261 cactaacggc aatatttttt accggcgatt taccgccttt attataaatc atctttaccg
     1321 tcttcttgcc gatagaacgg gagataatcg ccaaagttgg tttaaagaca taaaattcat
     1381 ccggatttac ccgcccctgg acgatatttt cccctaagcc ataaatggaa ttaattaaga
     1441 cagtattacg gaaaccggat tcagtgtcaa tggtaaacat aacgccggag gcacctaaat
     1501 cggagcgaat catcttctgt acgccgaccg atagagcgat ggaaaaatga tcaaagcctt
     1561 tgtcggcccg ataggaaatg gcgcgatcgg tgaataaaga agcaatacat ttctttacgg
     1621 ccaataataa ttccttctct cctacaatat ttaaaaaagt ctcctgttga ccagcaaaag
     1681 aagcgtctgg taaatcttcg gcggtcgcac tggaacgaac ggccacattc aaagccttgc
     1741 ttttattttc agcagacaag cgcttataag catcagcaat ttctttttta aaatcagccg
     1801 gcaattgaac ggataaaatt gcctcacgca ccgctttgcc gtgtgccatc aaattacgcg
     1861 tgtcttttgt gtcaaggtct tttaaaattt cttttatttt cttttttaat ccggtacttt
     1921 ctaaaaaata atgataagca tcggcggtag tggcaaaacc attgggcact ttaataccct
     1981 tatctttaag attgcggtac atttcaccca aagaggcgtt tttccctccg accgaaggta
     2041 cgtccttaat ggataactct tcaaaaaaac gaacaaaaac aggcttaatc ataaatatta
     2101 ttttaaaatt taattttccc caaaattata tttctattat agcacattct ttactttctt
     2161 ataataatac cataagataa taatgaaagc gggcgggtgt ggcggaattg gtagacgcac
     2221 ttggtttagg accaagcggg gcaacccttg agagttcgag tctctccgcc cgcacctatt
     2281 caaatgtttt actttctcca ctcattttta cctaattcca ttctttttag ttttgggccg
     2341 cttaccgttc attggtatgg tctattttta gttttaggga tggcggctgg cataacagtc
     2401 gcttggaaat taagctcatt ttatggaata gaaaaagaga aaatattgga tttagcgatt
     2461 tggctaatag tcagcggttt actgggagcg agaatttatg agttatttct agaatttcca
     2521 tattattatc aaaatcccac tgccgccttt aagttttggg agggaggact agcaattcat
     2581 ggcgccatta taggtggagc cattgccctt ttcctatttg ctaaacgtta ccacttatca
     2641 gcagctcgcc ttggcgcttt aagtgttacc ggcctgtcct tgggacaagc tcttggccgt
     2701 tggggcaact ggtttaatca agaacttttt ggattaccca ctaatttacc atgggggata
     2761 ccaatcgccg agataaaccg ccccttgcaa tatattaatg aaactttctt ccagccaaca
     2821 tttttgtatg aaagcctagg gtgccttctg atttttacat tcttattgtt tttaaattat
     2881 aaatggaaaa acaaaataga tgacaaaaaa gcgattatta taattatttt ttatctaatc
     2941 gcttattccc ttctaagatt tagcttggaa tttattaaag tggatattac gccaatgctt
     3001 tggggctggc gttggccgca aataatgagt ttaataatta ttaccaccag tctaatttat
     3061 ctttttataa actaccaccc ctttaagcat aaaactaaaa attaatatgt tgttaaaaat
     3121 atttaatcgg gttaataaaa gaaaaaatct tgttctcttg tatctcttgg ggctaattct
     3181 cgcgattgcc aatgctttac ccgcctacat tcagtctaat tttttgggac aaattttttc
     3241 gctatcatgg gtaagtatat tttttgccag cgctaattta attacagtct ttgccatcat
     3301 cttctttcct aaactcataa aaaaaatagg taacattgct tctacggaaa taatcttatt
     3361 tctttttatt ttttctcttc tgggaatgag cttggctaac catgcaattg ttatttttat
     3421 tgcttttatt cttctcagtg ttaccagcaa tctgatttgg attaatatgg atatattggt
     3481 ggaaagtttt tccgatgata cctccaccgg tctaaccagg accatctatt ttaccgccat
     3541 taacctcggc tggattattg ccccgtctct atcaagttat ttaatagaaa taggagatta
     3601 ctattgggta tttattgcgg cagctatttg tctggtgcca tttatggcta ttttaataaa
     3661 gaatcgtcat cgtttacgtg accgaggtcg ctaccgcaca caccccattc ttcctacaat
     3721 gaaaaagtta tggaaaaagc ctaatctccg tagcatcttc tttattgctt tattactcaa
     3781 tcttttcttc agcagcgcgg tggtctatat ccccgtttat ctacatcaaa atcttggttt
     3841 tgattggtcg gttttaggta taatgttttc tataatgcta gttccattca taatttttga
     3901 aattcccgct ggttggctgg ctgataaata ctggggggaa aaagaaataa tgggtaccgg
     3961 tttggctata attattatcg ctttattttt gttttttgta gttagagaag ctgacccctg
     4021 gttatggggt ggcctcttat tttttagtcg tgtcggcgct tctttagtag aagcgatgcg
     4081 tgaatcctat tttttcaaaa ttgttagcgc taaagatgtc cagtatatta atttcttccg
     4141 caccaccgcc cccttgggat atctactggg cacaatattg gccataataa taatatattt
     4201 ctacccctta caatacttat ttttatttat tgccctaatt atgacctctg gttttttatt
     4261 cctatacaca ataaaagaca gtaaataaaa aagttgacga atactgataa gttttctatt
     4321 atagtctcac cttagctgcg ggtatcatat aatggttatt atgtcagcct tccaagctga
     4381 aaatggcggt tcgattccgc ttacccgctc ataaaaatcc cccaatattg gggattttta
     4441 atatttacca aaatcataat caactatctt ctttctaata ggaccacgta ttaaaaaata
     4501 tagaccgccc ttaaaccact tccatagcac tgagcttacc ccctcttttt ccaatctcct
     4561 gacagaaaaa tataaataag cagattttaa catgcgatag cgcccgtgct tagacaagcg
     4621 tttgatatag tcataatctt caccaataaa aatgctttcg tcaaaaccat tttgataatc
     4681 atgcgctttc ttgtctacca taatgccaac accgaccgaa gctggaaaaa aaaattgacc
     4741 aataaaacag ataaaatgat aaaaaaattc aaacaaacga taaatatgtc ggggagaatt
     4801 aaaaattaaa taaaaaccgg cgacgcttaa atgacgactt ttaaattcgt tgtatatcgc
     4861 cggcaaaaaa ttatcgggaa ccatcgtgtc agcatccaaa aaaagaataa catcacccct
     4921 agcaaccgct gccccacgat tgcgttgcct agccggcgaa cgtttttcat cttgaataaa
     4981 aacacaagaa gctgatagcg ccgcctcttt agtgccatca ttagacatgc cgtcagaaac
     5041 aataatttca taatcagaaa aattttgctt ctttaaagaa gcaattagcc gcggtaaata
     5101 tttttcttca tttaaagtgg ggataactat agaaagcata ttaaaataaa gtacgggcta
     5161 aatcccaaaa acttttatcc ccatgtttat actgccaata ccaccactca actccccata
     5221 aataagcttg cttaaacttg gtatcaagcg catattgcaa attagcttta aattgatcga
     5281 gactaaaaga gtaatcggcc tcagttttag ataaatatat aattttaccc tgtggcaccc
     5341 acggttccgc ctgaagttcg acaatcaatc ttttatcagc aggaatattt actaaccaag
     5401 ctttaaatct atagaaccaa gcagggatag gataacgcat atagccaata accttattcc
     5461 aaattactct atacattgtc gtaccaaaaa catctccata tttagcctct ttcctccaca
     5521 tatttaattc tccaggcccg gaaaccatga tcgggcgttt atctaaagat ttaaccaaag
     5581 ccacctcctt agcaaaaaaa gcttcgtcac taggcggaca aataccaaaa gtatttaaaa
     5641 atggctcatt ctcaacttgc caatataaaa ttgaaggatt gtcttgatag cgccttaccg
     5701 tcttttctaa cattaataaa gctctttcct gaacatgagc aagatcttcc tgatttgtcc
     5761 actctggagc atggcactct ggccaacgag gcaaacgcca accaatattg ataataaacc
     5821 taacattacg cttagacccc tcgctaatca taaaatcata atcactgtaa ttaaaattac
     5881 ctttttccga ttcaatttca tcccaatata taggcagacg gatatttttt acttgtaaat
     5941 cgtctaagat ggcaaaatat aactcgcgcc aatttaggcc aatctccgta gcatattttt
     6001 ttgaaaaagt aacaccaaag aactctggat tagacttaat ttcaatatcc tgttcttttt
     6061 gaaaaatttt tataaataaa acagttgtca ctaaaaatac aataattatt agataaacta
     6121 tctttctttt aattttcata aagataaaaa ataaagacca atacttataa taaaaatagc
     6181 caatcccttc tgaaacaaaa ccctcttaga aatatcttct tttaaggctt ttggataaaa
     6241 aaatgaaata atcgctccta aaaatataat ccaagcatat tgcaccccct gcagagaatt
     6301 aacgatggct accgggccga gataaactgc atagttttgt aaaacaaaac caatggctcc
     6361 taatacttga ttaccaaaaa ttaaaccttg attgcgctta cgcttgggct ggcgcgtaaa
     6421 aatagctttt atttcccggc gcgctcgagg agaaaacaaa attaaactag ccgccaataa
     6481 ggcaccaagc ctagtccata aaaaagcgct aaaaaaatct tgtgaggcat aagcaaattt
     6541 actgccaaca aaaaataggg cgtaaaaaag accggataat aaagctaaaa atacgcttaa
     6601 taaagtggcg ctgtgtttta aatttaaaga tgtaaaaact ttttcccaaa aattctgacg
     6661 tttttccggc aaaaaaacca tcaccaataa tcctaataaa agcaaagaaa tagctatcaa
     6721 ttgattagtg ctgaacttat caccaaggag gaaaaaagat aaaggcagag aaaaaatagg
     6781 cgtagagccg ccgacaatta ccagagaacg agaagcttca cctcttctta gagatgcgta
     6841 caaaaacaat aaagctaaag caaataataa tcctaaaaat aaattaaacc agaaaaaaaa
     6901 tgttcccggc caagacaaaa accaaggagc gactaaaaaa ataagcgacc ccatcgcaca
     6961 ggcaataaaa gtataagccc tagcactacc aacaactttt tctactaaaa atttatcaac
     7021 taaatttgtg attgccaaca aaaaataagc tgataaagct acaaaaaacc aagtcataaa
     7081 ttaaaaatta gaaaatattt ctttttctat attttttacc ccttgatggc cggcgtgaag
     7141 ataagcattt aatgaggctc tggctaagcg cccatcatcc aaccagtcaa aaagccattc
     7201 atcaacaaac tctggattaa tctcgtcaaa agcggcgcca atagataaaa gccattcgcg
     7261 attgaatttt tcctgagcgc caaccggttc cgacataatt atcggtaaac ctaagccggt
     7321 ataaaaactt aattctgagg gcttagtcca taaaatatca gttttgcgta agcaaagatt
     7381 aaataaacga aaatattcca ttttttgtgg atgataaatt atgcggacat tatcattatc
     7441 gtttaatccc aaatttaaaa cagctttttt aaaatattca ttaacatccg cccgactacc
     7501 agccactaaa ttaaatttta tttttccgga ttttattgat gcttccaagc gtcgtagaat
     7561 taaagcccct atttctcgtt gcgcgccagc accgccgacg gcaaaagtta attctaacgg
     7621 cctataagcg ggctgaattt ctggaaacaa agagattagt aaacttttat atttatgaca
     7681 aaaaacccca cgcggatcta ggccacccaa tctgatagcc atatcatttt ttaaaagcga
     7741 atcatccgga ccaagattat caataggtaa gggaaatccg gttattctta ttttgtcaga
     7801 agaaacgcca tattccttca atctttcgcc aactcgttca ttcggacata aataaattat
     7861 tcgactagac tggggattaa tcggcgccca tgccctagaa atatcagcat cgcaaacaac
     7921 acaatatatc agcccttgat aattataata ttcagcgcaa taagccgcca caaaaaatgt
     7981 ggtgaccaag ggtaaaactt ctttatttag agattcaatc aattttttac ccacaccctt
     8041 tttaacggct tgaaagaata gcttttgttg taaagtcgac ttagataaat ctcggcgagg
     8101 ataaaaagca tcaatctttt gaaaataatc catagtgcga aacactaatt ctcctaagac
     8161 agggactcgt ttgaaataag atattttctc gtatgatttt tgactgttaa gccaataaga
     8221 tttttcccaa ttatcaattt ccggataatc attaagatta atgatcccgt ttcgacttaa
     8281 cgataataaa ggataagctg ctcgttggtg gccataaccc atatcggctg ccactaaata
     8341 aattttttta ttatccataa acacagttga ttaaaattat aattacatta tacattatac
     8401 tataaaaaaa tcgcccctga aagggcgatt ttagttattt agttattttg aagttgtaat
     8461 tagggacgcg cccgcccctt gaatatactg ataggcataa agagcggctg aagtagcctg
     8521 acccatagca atagtaatct gtttaaaatc actggcaaca acatcaccag cggcaaataa
     8581 gccgggggtg cgggtggcat atttattatc tactaatatt tgtaaattct catcacgatc
     8641 aactaaatcc gccactaaat tagtactggc gatacggccg atttcaataa aaacgccatc
     8701 taaagacaaa gaaatgtctt gaccagaatc attttgataa accaaagact caaccttatc
     8761 tccgccctta atctcacgag tttgagcgcc agtaataatt tcaatattat cacgattttt
     8821 cacctcatca accaaagaat caaaggcctt aaaagtagcg cctcgggtaa ttagatacac
     8881 cttagcggca attttagaaa gcacttcagc cgcatcaaaa gcagaattac cactaccgat
     8941 taccgccacc gttttgcctt tataaagagg accgtcacaa ttagcgcaat aagaaacgcc
     9001 cttaccgtta aattcaacct ctccgggcac agccaaacga cggggtgaca aacccatagc
     9061 aataattacg gttttagctt catatttatc acggtttgta aaaaggagaa aactgccatc
     9121 agtttgtttt tctacccgct ttacctcagc attctttatt tctactccag aagctaaagc
     9181 gtgctcatta aatctagaaa ttaattcaaa actattaata ctagtaaaac cgggataatt
     9241 ttcaatttta cctgcccaaa taatttgccc cccaggctct tttcctaaaa ttaaagtctt
     9301 catttctctt ctggcggcat aaataccagc ggtaatacca gctggaccct ggccaataat
     9361 aatagtgtcg tacataaatt atttaattaa taataaataa tactatatat tatacaatga
     9421 ctaataataa aaggcaaaac taaatatttt ggctaatt
//
LOCUS       DAOQSA010000027         9210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_6932_length_9210_cov_8.652977, whole genome shotgun sequence.
ACCESSION   DAOQSA010000027 DAOQSA010000000
VERSION     DAOQSA010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9210
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6932_length_9210_cov_8.652977"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..73)
                     /locus_tag="PKK65_03325"
     tRNA            complement(2..73)
                     /locus_tag="PKK65_03325"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38..40),aa:Glu,seq:ttc)
     gene            216..1253
                     /gene="tsaD"
                     /locus_tag="PKK65_03330"
     CDS             216..1253
                     /gene="tsaD"
                     /locus_tag="PKK65_03330"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNU81738.1"
                     /translation="MYILGIESSCDESAASVLKASGQKTEILSNVIASQINIHAQYGG
                     VIPEIAAREHVLHILPTIDSALKQAKITHKDIKALAVTQGPGLITSLIAGVESAKALS
                     LIWKKPLIPVHHVIGHIYANFINRPQAPKFPLLALVVSGGHSNLIYMKEHYQFKIIGE
                     TRDDAAGEAYDKAAKMMGLSYPGGPIIAKYAEEYRQSGQKSTIQLPRPMLNSPDFDFS
                     FSGLKTALLYQLQKDKNWKKRLSEYCFAFEQAIIETLIHKTLKAANKYQPQSIVLAGG
                     VAANSALREQLSSNIKDKLPKIAFFLPEKQYTTDNAAMIASAGYFNYYKHKNKAFLDY
                     SRFKARCDLKI"
     gene            1305..1378
                     /locus_tag="PKK65_03335"
     tRNA            1305..1378
                     /locus_tag="PKK65_03335"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1339..1341,aa:Cys,seq:gca)
     gene            2085..3149
                     /gene="ychF"
                     /locus_tag="PKK65_03340"
     CDS             2085..3149
                     /gene="ychF"
                     /locus_tag="PKK65_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005828368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNU81739.1"
                     /translation="MSLSLGIVGLPNVGKSTLFNILTKKNVETANYPFCTINPNVGIV
                     KVPDKRLDDLAAISKPAKIIPTAIKFVDIAGLVAGASKGEGLGNQFLAHIRECDAICE
                     VIRNFQDKNIIHVSGEVNPQADKETINLELILADLGSIEKKLDKTRRESKSGNKELIS
                     QTIILEKIYQHLEKGLAARSLEFVEEELPFVKTLNLLTIKPIIYLFNVDDLKNIPELG
                     ELDGEIIAINVKMEEEITALPEEEQAEYISELGLDQSGLDKLIATSYKLLGLDTFFTT
                     GPDETRAWTIKHGTKAPEAAGVIHTDFIKNFIRAEVINWEQFITAGSEAKAREQGLIR
                     TEGKDYIVRDGDVCNFLVNR"
     gene            complement(3199..3759)
                     /locus_tag="PKK65_03345"
     CDS             complement(3199..3759)
                     /locus_tag="PKK65_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81740.1"
                     /translation="MSDAMDILPKGGKEFSALGFKKKLKTAVKGNSSMRSLSSNQEAI
                     NKAVYKARRLITKGAFKNAQQQSVLSQINKSASLTASQKTLVRKVLKHLGESGHISHA
                     RINRADEQEIEAPHLANQPQLANRSGLSGIASPDIKRSATRPMVSISQVQAERRGSGL
                     AGGIGAVNPNTPAPPPSRPPMIPLSR"
     gene            complement(3770..4948)
                     /locus_tag="PKK65_03350"
     CDS             complement(3770..4948)
                     /locus_tag="PKK65_03350"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNU81741.1"
                     /translation="MDYIFILGTHPAWSVAEIAAVLGESDLYWENERILIKKDAEIAA
                     ENLIKRLGGTLKIAQIEAFVSADIPFPKLAKQCAELLLKKAARGDGKLVFGLSDQGVG
                     GRFAADKLGLNIKKELKNFNISSRLVTSQEKELSSVVVGQNKLLNRGAELIFFRHDQD
                     LFIARTLAIQPYKDLSRRDFGRPSRDDYSGMLPLKLARMMINCAAPRSFSDILLDPFC
                     GSGTIVTEAMLMGYRQIIGTDLSEKAIKNSRANAEWVRELYGLDIYPKFHIKNATRLA
                     QFLKPKTVGTIVSEPYLGPQRGLDNIEAVKAELQTLYSQALDNFYQILSPGGRVVMVW
                     PVFFGREFLKPALDKWKPINALPEKFSHIPWVKLSYRDTLLYHRAGQRVWRELIILEK
                     "
     gene            5204..5728
                     /gene="rpsF"
                     /locus_tag="PKK65_03355"
     CDS             5204..5728
                     /gene="rpsF"
                     /locus_tag="PKK65_03355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNU81742.1"
                     /translation="MSKSKASGPSSYEVLFIVANNYTEEEAKTIVSKVEAIISENGGN
                     ITYREFWGKKKLAYVIKQNHYGYYSLCEFNAKRETIAKLDRTLRLSHEVLRHQIISIK
                     AVSDEERAKIKEKQEQSAVKNEKKEEKTGKEKFQKVMTSKNEKTEKNDSNKADLKDLD
                     EKLEGILNNTQDLL"
     gene            5780..6223
                     /locus_tag="PKK65_03360"
     CDS             5780..6223
                     /locus_tag="PKK65_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNU81743.1"
                     /translation="MNLNKAMIIGNLTRDPEVRTTPSGQNVASFSVATSLIWTDNAGQ
                     QQKKTEFHNIVAWRKLADICGQYLKKGSKVYVEGRLQTTDWIGNDGVKKYRTEIVAEN
                     MIMLDRAGGNENFGSGPATPRRQEEELPTINADEEEEIRVENIPF"
     gene            6250..6462
                     /gene="rpsR"
                     /locus_tag="PKK65_03365"
     CDS             6250..6462
                     /gene="rpsR"
                     /locus_tag="PKK65_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNU81744.1"
                     /translation="MNNKKHKDCFFCVNQMDVDYKDVRTLRRFVNFYMKILPGDRTGI
                     CAWHQRKLTQAIKRARVMALLAPTHK"
     gene            6477..7037
                     /gene="efp"
                     /locus_tag="PKK65_03370"
     CDS             6477..7037
                     /gene="efp"
                     /locus_tag="PKK65_03370"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNU81745.1"
                     /translation="MLNFNEIKTGKVILLNEEPYVIIKTDHHKMGRGGAVLKVKCRNL
                     INGNILERTYQGAEKAEEAETERKKANFMYKDKDEAYFMDNESYEQFNLHVDEIGDKV
                     QFLKEGTDVNVLYFNNKPVAIDLPIKMEFKVVSAPPGVKGNSAGNVNKQVELETGAMI
                     NVPMFIEEGDIIRLNTDTGEYVERAN"
     gene            7144..8514
                     /locus_tag="PKK65_03375"
     CDS             7144..8514
                     /locus_tag="PKK65_03375"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNU81746.1"
                     /translation="MEILTITSLILFTVIAAIRLDWALFLILAALPAYLLRFNLGPIP
                     MTTLEAMIIIVFTSWFFRARPWQRFKKKDWQERRQKYPYSLEIIGILITAWSGLAIAG
                     FDNASLGIFKAYFLEPIMLYLVVINRGQGSYRKFIWPLALSAALVSLVTFFQQLTGLF
                     IFNDFWALASQRRVTSVFPYPNAVGLYLGPIIFLMAGAFASYPRRSNLLTASKKILLL
                     LIIISAIAAIFFAKSEGALIGLAGGAMIVSLLYNKRSRQIAAGIITAGILVLIISNPA
                     WQYFQKKATLMDLSGQIRRQQWKETMQMLKNGRLISGAGLSNYQEAVKPFHQEGIFVK
                     NDDPDWYRKVVWNDEYKKQVWQPVEIYLYPHNIFLNFWTEIGLFGALLFTWLIGRYFY
                     ESIRLKDKLERSAKMINLGLIGAMSTIVIHGLVDVPYFKNDLAIIFWLLIAMFGLLKI
                     KQKQTE"
     gene            8690..8790
                     /locus_tag="PKK65_03380"
                     /pseudo
     CDS             8690..8790
                     /locus_tag="PKK65_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017863367.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridylate synthase"
     gene            9123..9209
                     /locus_tag="PKK65_03385"
     tRNA            9123..9209
                     /locus_tag="PKK65_03385"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9157..9159,aa:Ser,seq:cga)
BASE COUNT         3016 a         1905 c         1740 g         2549 t
ORIGIN      
        1 gtagtctata ggggaatcga acccctgtta ccaggatgaa aacctggtgt cctaaccact
       61 agacgaatag accagataaa aaaatagaaa cttagttcct aatttcaaaa atattgtatc
      121 gatattttcc ggccttgtca aataaaaaat attattgtta aagatttctc ttgcctaata
      181 ttgcctttat tctaaaaaac cttacaatat aagatatgta tattctcggt attgaaagta
      241 gctgcgatga aagcgccgct tccgttttaa aggcctccgg ccaaaagacg gaaattttaa
      301 gcaacgtcat cgcttcacag ataaatatcc acgcccaata tggcggcgtg ataccggaaa
      361 tcgccgctcg cgagcatgtt ttacatatcc tcccgacaat tgactccgct ttaaagcaag
      421 ctaaaatcac tcacaaagac ataaaagcgc tggccgttac tcagggtcct gggctaatta
      481 cctcccttat cgctggcgtt gaaagcgcta aagctttatc tttaatttgg aaaaaacctt
      541 taattcccgt tcatcatgtt attggtcata tttatgccaa ttttattaat cgtccccaag
      601 cgccaaagtt tccccttctt gctttagttg tttccggagg acacagcaac ctgatttaca
      661 tgaaagaaca ctatcaattc aaaattattg gcgaaaccag ggatgatgcc gccggcgaag
      721 cttatgataa agccgctaaa atgatggggc tcagttaccc cggcggacca attatcgcta
      781 aatacgccga agagtatcgc caaagcggtc aaaaatctac aatccaactc ccccgaccaa
      841 tgttaaattc tcctgatttt gatttttctt tttccggtct aaagaccgcc ctattatacc
      901 aattacaaaa agacaaaaac tggaaaaaac gcttatctga atattgtttt gctttcgaac
      961 aggcgattat tgaaactctg attcacaaaa ctctgaaagc ggccaataaa tatcaacccc
     1021 aaagcattgt tttagcggga ggagtggctg ctaatagcgc cctaagggaa caattatctt
     1081 ctaatattaa agataaacta cctaaaattg cctttttcct accggaaaaa caatatacta
     1141 ccgacaatgc cgccatgatt gcttctgctg gctattttaa ctattataag cataaaaaca
     1201 aagcattttt agattattct cgctttaaag ctcgttgtga cctaaaaata taaataattt
     1261 gcttttttag taataatcct ttaaaataat cgcataattt atctggctcg atggtgaagt
     1321 ggtctaacac tgcagtttgc aaaactgcca ttcgtgggtt cgactcccac tcgagcctct
     1381 tttatggccg aggctttagt ctcattaatt tcagattaaa gctttttatt tatcctaaaa
     1441 gatgtgttaa aatttgtgga taacttgtgt ttcctgtgta taaaaattat tttttagtct
     1501 agaaatacct agacttaaaa agaaaaaata tcatttattt tatctaatat taaataatat
     1561 atttaatatt ggcagaataa acaccaatag acttgtttta tccccagata tatgctataa
     1621 tactaacagt tagaagttct ctttactaat ctcttgacta tttatcggcg ctaacacgat
     1681 aaagaacagc aaaaattgct tacggagcag aaaaaagtgc taatcaaaaa gaagaaaaaa
     1741 acttaataga atctaaaaaa gaaattttat caagttttta tctgaaaatt agcaaaatca
     1801 attaaaataa aaacattctg caaagaaatt ggtaagagaa agcttgaagt tagcaaagct
     1861 ccaaacataa tcctggaaaa aattggcaaa aagccaaaaa caattcacca ggattggagc
     1921 tcaaacgagg cctattattt atatacttta ttattatatt attatattat tataaagagc
     1981 ggcccatttg ggctcctttt ttattaggaa aacaatcttc aaatttgctt ttttacctta
     2041 tttctgataa acaaataaga gttataatgt atttagattt atttatgtct ctttctcttg
     2101 ggatcgtcgg tttgcctaat gttggcaaat caactctatt caatattctc accaaaaaaa
     2161 acgtagaaac cgctaattat cctttctgca cgattaaccc taatgtcgga atcgtaaaag
     2221 tgcctgataa acgcttggat gacttagccg ctatctctaa accggctaaa attattccga
     2281 ccgccattaa attcgtcgat attgctggtt tggtggcggg tgctagcaaa ggcgaaggtt
     2341 taggtaatca atttctagct catattcgcg aatgcgatgc aatttgtgaa gttattcgca
     2401 attttcaaga caaaaatatc atccatgtca gtggcgaagt caacccgcaa gcggataaag
     2461 aaacgataaa tttagaactt atcctggccg atctaggcag tattgaaaaa aaacttgata
     2521 aaacccgccg agaaagcaaa agtggcaata aagaactgat tagtcaaaca ataattttag
     2581 aaaaaatata ccaacattta gaaaagggtt tagccgcccg ctcgcttgaa tttgttgaag
     2641 aagaattgcc ctttgttaaa actctcaatc ttctaacaat taagccaata atctacctat
     2701 tcaatgtcga cgatttaaaa aatatacccg aattaggtga gctcgatggc gaaatcattg
     2761 ccattaatgt aaagatggaa gaagaaatta ccgccttgcc ggaagaagaa caggctgaat
     2821 atatcagtga actcggtcta gatcaaagcg gattggataa actaatcgcg acttcatata
     2881 aactactcgg tctcgatact tttttcacta ccggaccaga cgaaaccaga gcctggacaa
     2941 taaaacatgg cactaaagcg ccggaagcgg ccggggttat ccataccgac tttattaaaa
     3001 actttatccg cgctgaagtg ataaactggg aacaatttat tactgctggc agtgaagcta
     3061 aagcaaggga gcaaggatta atccgtaccg aaggtaaaga ttatatcgtc cgcgacggcg
     3121 atgtctgtaa ttttttggtc aaccgctaat tttaattttc tctatttaaa aacccgccac
     3181 taccggcgga tttttatatt atctagacaa aggaatcata gggggccggc tgggaggagg
     3241 ggcgggagta ttgggattaa ctgcccctat gcctccggcg agaccagagc cgcgacgttc
     3301 agcttgcacc tgactaatgg aaaccatcgg acgtgttgca gaacgtttaa tgtctggaga
     3361 ggcaatgccg ctcaatccgg agcgattggc taattgtggc tgattggcta aatgcggagc
     3421 ttcgatttcc tgttcatcag cgcgattgat cctagcatga gaaatatgac cgctttcgcc
     3481 aaggtgcttt agaactttcc ttactaatgt tttttgactg gccgttaagc tggcactttt
     3541 attaatttga cttaaaacac tctgctgctg agcattttta aaagcgcctt tagtaataag
     3601 acgcctcgcc ttatagacag ccttattaat cgcttcttga ttggaagaca aagagcgcat
     3661 cgagctgtta ccttttaccg ccgtcttcag ctttttttta aagccaagag cgctaaattc
     3721 tttaccgcct ttaggcagta tatccatagc atcgctcata aatttaaaat tatttttcta
     3781 aaattattaa ttcccgccat accctctggc cagcgcgatg ataaagcagg gtatcacgat
     3841 aacttaattt gacccacgga atatgagaaa atttttccgg taaagcgttg attggcttcc
     3901 acttatctaa ggctggtttt aaaaactcac gaccgaaaaa taccggccaa accattacca
     3961 ctcgacctcc aggagacagg atttgataga aattatctaa ggcttgagaa taaagagttt
     4021 gtaattccgc ttttaccgct tcaatattat ccaaaccacg ctgaggacct aaataaggct
     4081 cagaaacaat tgtccccact gttttcggtt ttaaaaattg ggccaaacgg gtggcatttt
     4141 taatatgaaa tttgggatag atatcaagac cataaagttc acgtacccat tcggcgttag
     4201 ctcggctatt ttttatcgct ttttccgata aatccgtacc aataatctgg cggtagccca
     4261 tcaacatcgc ttcggtgaca attgtgccag agccgcagaa aggatcaagc aaaatatcgg
     4321 aaaaactacg cggagccgcg cagttaatca tcatccgtgc tagcttcaga ggtagcatgc
     4381 cggaatagtc atcacgggaa ggccgcccga aatctcgacg cgataagtct ttatagggct
     4441 gaatagctaa agtgcgggcg ataaataaat cttggtcatg acgaaaaaaa atcaattccg
     4501 cccctctgtt caacaactta ttttgcccaa cgaccacgct cgacaattcc ttctcctggc
     4561 tggtaaccag acgggaggaa atattgaaat tttttaactc ttttttaata ttcaaaccaa
     4621 gcttatccgc ggcaaaacgg ccgccaacac cctgatcaga caagccgaaa actaatttac
     4681 catccccgcg cgcagctttt tttaatagta attcggcaca ttgtttagct aatttcggaa
     4741 aaggaatatc agcagaaaca aaagcttcaa tttgagcaat ttttaaagtc ccgcctaaac
     4801 gtttaattag gttttccgcc gcaatttccg catccttctt gattaatatg cgctcatttt
     4861 cccaatacaa gtcggattca cccaaaacag ccgcaatttc cgccaccgac caagccgggt
     4921 gagtgcctaa aataaaaata taatccatat actttcataa tgccctaact cttgcttttt
     4981 ttcaagcttt atgataaatt tagaagacaa aaaaatattt atatatcttt cccatattct
     5041 tgtcttaaga cagaataatt ccgtttcttt aaataatgac ggaattaaag gtattcctaa
     5101 gaagctcaag ttctgaagaa tacttgggtt tttgtttaat aaaggcagtt taaaacaatg
     5161 ttaagcccag cttacattaa ttttaagctc attatgctaa attatgtcta aaagcaaagc
     5221 atcaggacca agcagttatg aagtgctctt catcgtggcc aacaattaca ctgaagaaga
     5281 agcaaaaaca atcgtcagta aagttgaggc catcatcagt gaaaatggcg gcaacattac
     5341 ctaccgagaa ttctggggca agaaaaaact agcctatgtc atcaaacaaa atcactatgg
     5401 ctattatagt ctctgcgaat tcaacgctaa acgtgaaacc attgctaagt tagatcgcac
     5461 tctacgcctc tcccacgaag ttctgcgtca tcaaattatc agtatcaagg cggtcagcga
     5521 tgaagaacga gcaaaaataa aagaaaaaca ggagcaatcg gcagtaaaga atgaaaagaa
     5581 agaagaaaaa acaggcaaag aaaaatttca gaaagtaatg accagtaaaa acgaaaaaac
     5641 cgaaaaaaac gacagcaaca aggctgattt gaaagatttg gacgaaaagc tggagggcat
     5701 attaaacaac actcaagatc tcttataaac taacggccta attttataag cgcgtttata
     5761 aaattaagtt cccaacatta tgaacttaaa caaagctatg attatcggca atttgacacg
     5821 cgacccggaa gtgcgcacca ctccctcagg acagaacgtc gcctctttca gcgtcgccac
     5881 cagcctcatc tggaccgata atgccggtca acagcagaaa aaaacagaat ttcacaacat
     5941 tgtcgcctgg agaaaattag cagatatctg tggccaatat ctaaaaaaag ggtctaaagt
     6001 ctatgttgaa ggcagattgc aaacaaccga ctggatcggc aacgacggcg tcaaaaaata
     6061 tcgcacggaa atcgttgcgg aaaatatgat tatgcttgat cgcgccggcg gcaatgaaaa
     6121 ctttggcagc gggccggcta ctccgcgccg gcaggaagaa gaactgccga ccatcaacgc
     6181 cgatgaagaa gaagaaatcc gcgtcgaaaa catacccttc taaaaatcaa cctcaaaaaa
     6241 taatcttata tgaacaacaa aaaacacaaa gactgctttt tttgcgtcaa ccagatggat
     6301 gtcgactata aggacgtccg caccttgcgc cgcttcgtta atttctacat gaaaattctt
     6361 cccggcgacc gcactggcat ctgcgcttgg catcagcgca aactgacaca agcaattaaa
     6421 cgcgcccgtg tcatggcctt attagcaccg actcataaat aattagctgc accaatatgt
     6481 tgaacttcaa cgaaatcaaa accggcaaag ttattttgct aaacgaagag ccttatgtaa
     6541 tcattaaaac cgatcatcat aagatgggac gcggtggcgc tgtcttaaag gtaaagtgtc
     6601 gtaacctaat aaacggcaac attctggaaa ggacttatca aggcgcggaa aaagcggaag
     6661 aagcggaaac cgagcgcaaa aaggcaaact tcatgtataa agataaggac gaagcctatt
     6721 ttatggacaa tgaaagttac gaacaattta atcttcatgt cgatgaaatc ggcgataagg
     6781 tacaattctt aaaagaaggc acagatgtta atgttttata cttcaataac aaaccggtag
     6841 ctatagactt acctattaag atggaattta aagttgtttc tgctccccct ggagttaaag
     6901 gcaactctgc tggcaatgtt aacaaacaag tagaattgga aaccggtgcc atgatcaacg
     6961 tcccgatgtt tattgaagag ggcgacatta tccgtctcaa caccgatact ggtgaatatg
     7021 tagaaagagc caattaatga ttgatgtaaa gatagtaaca acaccttaag ctgaaaaact
     7081 taaggtgttt ttataaaaaa ataacagtcg acagtccctt gattcgtgct ataatcaaat
     7141 tatatggaaa tattgacaat aaccagttta attttattta cggtcatcgc tgccattcgc
     7201 ctggactggg cgcttttttt gattttagcc gctctccctg cctatctatt gcgcttcaat
     7261 ctcggaccga taccaatgac gactttagaa gcgatgatta tcattgtctt cacctcttgg
     7321 ttttttcgcg cgcgcccttg gcaaagattt aaaaaaaaag attggcaaga gagacgccaa
     7381 aaatatccat attctctaga aattattggc attttaatca ccgcttggtc tgggttagca
     7441 atagctggct ttgacaatgc ctccttagga atatttaaag cttattttct ggagccaata
     7501 atgctctacc ttgtggttat taaccgcggc caaggatcct atcgaaagtt tatctggccc
     7561 ttagctcttt ctgctgccct tgttagttta gttacatttt ttcaacaact aactggatta
     7621 tttattttca atgatttttg ggctttagct tcccagcgcc gcgttacttc cgtttttcct
     7681 tatcccaatg ctgtcggcct ttacctaggg ccaattattt ttctgatggc cggagctttt
     7741 gcttcctatc cacgccgcag taatctgcta acagcctcta aaaaaatctt acttttatta
     7801 ataattatat ctgccatcgc ggccattttc tttgctaaat cggaaggagc tttaatcggc
     7861 ttagccggag gcgcaatgat cgtttcttta ttatataata aacgttcacg tcaaattgcc
     7921 gccggtatta ttaccgccgg catcttagtt ctaataattt ctaaccccgc ctggcaatat
     7981 tttcaaaaaa aagcgactct aatggattta agtgggcaga ttcgccgaca acaatggaag
     8041 gaaactatgc aaatgctgaa aaacggacgc ttaattagcg gagctggctt aagcaattat
     8101 caagaggcag tcaaaccatt ccatcaagaa ggcatttttg taaaaaatga tgaccccgat
     8161 tggtatcgca aagttgtctg gaatgatgaa tataaaaaac aagtctggca gccggtagaa
     8221 atatacctgt acccgcataa tatcttcctc aacttctgga cagaaatcgg tttgttcggc
     8281 gccctactct tcacctggct catcggtcgt tatttttatg agtcaatacg actgaaagat
     8341 aaattagaac gatcggcaaa aatgattaat cttggcctca tcggcgcaat gagtaccatt
     8401 gttatccatg gattagtcga tgttccttat tttaaaaatg atttagcgat tatcttctgg
     8461 ctgttaattg ctatgtttgg attactgaaa ataaaacaaa aacaaacgga atgattgctc
     8521 taaccaaata tctggctcaa accggtatta cgagcgccgt caagccgaaa ttctaagaca
     8581 aggtgttaat ggcaaacgag cagctttaaa caatatggtg gcgggagatg aagaaatatg
     8641 agtcaaaaaa actgataaac ggaaaaatag catcagcgat gaagataata tcttcagcct
     8701 gttgccgccg ccatataaaa atctattatt atcaccggcc gccttgacaa aaacagtcgc
     8761 ggtttgcttt tattaactaa tgatggcgaa tcgggcgtga taagaaatat ttagtgcgct
     8821 tcgccggcga agaccggatg aatttgaatg aacataatct ctatgctcgc gccaaagaag
     8881 gtgtctactc cgaagatgga gaaaaattaa acaccaaaaa tatccgcaaa gaagaaaacc
     8941 actaagcagt tacttggacg aaggcaaaaa ggtatagcca ccgattgttt gccaaaatga
     9001 gcctgaaagt taccgattta caacgtatta gtatcgaaga cttaagtcta aacggcttac
     9061 ctatagaaac ttttagggta ttatccgaaa aaaatattaa gaagatagta aaataataat
     9121 aaggagaggt gactgagtgg ttgaaagtac cgctctcgaa aagcggcaag ggtgaaagcc
     9181 cttcgggagt tcgaatctcc ccctctccgc
//
LOCUS       DAOQSA010000028         7241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_10048_length_7241_cov_8.527554, whole genome shotgun sequence.
ACCESSION   DAOQSA010000028 DAOQSA010000000
VERSION     DAOQSA010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7241
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10048_length_7241_cov_8.527554"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..530)
                     /locus_tag="PKK65_03390"
                     /pseudo
     CDS             complement(1..530)
                     /locus_tag="PKK65_03390"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            756..1667
                     /locus_tag="PKK65_03395"
     CDS             756..1667
                     /locus_tag="PKK65_03395"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HNU81747.1"
                     /translation="MYAIWNLTKVCPWDCSFCCMSAIKVGQVNGGESLNFSEKIKVLE
                     ILATHKMKIDFSGGDPLYNPEDFAIVERAVEIMPRDFIDISMTGVDFNEKKLALLKKV
                     GKVEISIDNVPKDSNRFRPKGFNLSAINILKTLVEENIFCSAVTTLYPLSADKNNLKR
                     LYELLCINQVPKWNILRFYPVGRGLKLSNLYIDDEKTLELMDFLDSLGSGETEIVFQH
                     SLKILRGEYQCHAAIKSIGILPNGTVTACGWALDKNSNPLPGFCLGKLPDDDFDVILE
                     KAQVDMGYSERQSYCRILNYLKSQEAI"
     gene            1700..2452
                     /locus_tag="PKK65_03400"
     CDS             1700..2452
                     /locus_tag="PKK65_03400"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNU81748.1"
                     /translation="MPSKLPEADYCINPYTGCLHKCVYCYACFMKRFTNHSENWGEFL
                     DVKVNAANILNEELVKKSKRKGSILLGSVTDVYQPAEKKFQMTRKILEVLAKYDFPVS
                     ILTKSELVVRDINLLKKIPDCEVGLTITSLDDRISKIFEPGASVPVKRLDALTILKNS
                     GLKTYAFVGPILPGLTDLKLIIPALQGKIDFIMFESLNYGKFNSDNINQAYNEAGVNI
                     PVFRDIDWSEIESEARELSQIYKITVKGFYKH"
     gene            2534..2986
                     /locus_tag="PKK65_03405"
     CDS             2534..2986
                     /locus_tag="PKK65_03405"
                     /inference="COORDINATES: protein motif:HMM:NF039500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5680 domain-containing protein"
                     /protein_id="HNU81749.1"
                     /translation="MKDFKRFLIKAKKNTYALSGERGESVLADGAKELNYKSENFYYR
                     DRYYGSDPFVGEEVVFLNNKPFWVMNYSGRCLETIISKSEIYSFLKKCLKKVNANNPF
                     RGPSEYQSGEYIYKNKTKGDIDNFYGEELICYKNKKIYELKYHGGLVE"
     gene            3719..4102
                     /locus_tag="PKK65_03410"
     CDS             3719..4102
                     /locus_tag="PKK65_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HNU81750.1"
                     /translation="MENKLKPKILAEIKAELLARKQQILESLEDVSQKNPHEADNLGS
                     KFPEYGDKPDENAQEISDYVADAAAEKVLEKTLADIDSALNRIEKGTYGICKYCGQPI
                     SPKRLQARPVASACIACKTELQENE"
     gene            4158..4568
                     /locus_tag="PKK65_03415"
     CDS             4158..4568
                     /locus_tag="PKK65_03415"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNU81751.1"
                     /translation="MFFIGDRLLKNLALNIGAEQYYNILDPWLKFRLAFNPYIAFSLP
                     IGGQALNIIITTIIAALVFIIFYLIFNKNKQNGLIIPLTFILFGSISNLLDRYLYGAV
                     VDYFDFRYFTIFNLADIIISIGAVSALIVAVKKK"
     gene            4572..5264
                     /locus_tag="PKK65_03420"
     CDS             4572..5264
                     /locus_tag="PKK65_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81752.1"
                     /translation="MFNSFSFEKINNFENKGSILEFEYFGEINKRAYSQAVDEYLNPE
                     LYKIKSQEDVERVFLRKNKEKEDYANSFKNDSYIKFKDAMAIVEKCQFGNPEMPNAIF
                     ARVLRKEISNLLGDEYKVKFFTAVGSHLDTKHGVDAFIKVYDKNEKELAQVTLDISGR
                     EKGASKADLLIVVSREERDCYDFDSDNFDKEKFNNRIKNEALRIKEIITKNRKTSGEY
                     PKSSYGQKQVVK"
     gene            complement(5459..5908)
                     /gene="tsaE"
                     /locus_tag="PKK65_03425"
     CDS             complement(5459..5908)
                     /gene="tsaE"
                     /locus_tag="PKK65_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNU81753.1"
                     /translation="MRNKQTYISHSPEETFILGKNLATTLQGGEILAMSDDLGVGKTV
                     FVQGLASGLGVKAKVNSPTFTIMKLYPAKKGKIKQLCHIDAYRLSSGRELLDIGANDY
                     LGREDVVSAIEWAEKVRTAWEDYSVITIKITSISENERKIEIKKEIS"
     gene            6288..6941
                     /locus_tag="PKK65_03430"
     CDS             6288..6941
                     /locus_tag="PKK65_03430"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HNU81754.1"
                     /translation="MEKITFKTEWRALLIIIATIALSYWAYPQLPTTVASHWNFQGEV
                     DGWMSKNFHSIFFPALLVVMYLGFLIMPYIDPQKERYAEFANVYRLMRDASLFILLGV
                     FTVATFYNLSYSLNVSAIVAGLIGALMIIMGNYFGKIKRNWFIGIRTPWSLSSENVWN
                     KTHRLGGRFFMVWGLGIAIAPWLDFRVAMIILLAGLAAIIIGINLYSFILYRQEKKK"
BASE COUNT         2396 a         1266 c         1292 g         2287 t
ORIGIN      
        1 cccgctaatg tattgagggc aaatttacct tgacgagaca ccaccgaatc gccttcccaa
       61 tgacccagtt cttttcttaa ctcgatttct ttcggcctct ccgatatcga aatcgcccca
      121 atgattttct gcctctgcgg cctaaatgga atatattttg gatatctagt tttatgtctt
      181 ctttttaaat agattcgcaa atcttcaccg atacagcggc catagccctt tcgacaatac
      241 tgggaatata tatatttata tatcgcctca tagctgattt tagttccggg gatacctctt
      301 ccgattcgac cggctatctg ttccggagaa taatgagact ggagcatggc cttaacataa
      361 tccctaattt cttggctctt taatctttct cgccgccccc gattcagtat tctttcttgc
      421 gccctttctt gggccagtcg ggggacatac ttcggtctga gtcctaatgt gttccgtttt
      481 aactcacggc tgatagtcga gaccggccta tttagttcca aggcgatgct tcggagcgat
      541 cacttcgccc agacccctgc ccgaatagtc tctcggtctt caatacttaa ttgtttatgt
      601 ttgtttggca tatacctgta attatacagg tgttgcgttt acttgttgaa ctcgccaagt
      661 tatatcgcca agttatattt tgacaaatag caaaataatg gtattatgat tagttcttta
      721 aaataaaaat aacaaacata aaaaaggagg ttatcatgta tgcaatttgg aatttaacaa
      781 aagtatgtcc ttgggattgt tctttttgct gtatgtcggc aataaaagtt ggtcaagtta
      841 atggagggga gagtctaaat ttttcagaaa aaattaaagt tttagaaatt ttagcaactc
      901 ataaaatgaa aattgatttt tctggcggtg atcctctcta taacccagaa gatttcgcta
      961 ttgtggaaag agctgtcgaa attatgccta gagattttat tgatattagc atgaccggcg
     1021 tggactttaa tgaaaaaaaa ttggccttgc taaagaaggt tgggaaggtt gaaatcagta
     1081 tcgataatgt tcctaaagat tctaatcgtt ttcgtccaaa aggctttaat ttgtcggcca
     1141 ttaatatttt gaaaaccttg gtggaggaaa atattttttg ttcagccgta acgacattat
     1201 atccattatc agccgacaaa aacaatctta aaaggctcta tgagctttta tgcatcaatc
     1261 aagtcccgaa atggaatata ttgcgtttct atccagtcgg taggggttta aaattatcca
     1321 atttatatat tgatgatgaa aaaactttag agttaatgga ttttcttgat tctcttggga
     1381 gtggggaaac agaaattgtt ttccaacatt cgcttaagat tcttagggga gaatatcaat
     1441 gtcatgctgc cattaaatca attggaatat tgccaaacgg gacagtaacg gcctgtggtt
     1501 gggcgttaga caaaaacagt aatcctttgc ctggcttttg tttaggaaag ttgccggacg
     1561 acgattttga tgttattctg gagaaagcac aggttgatat gggctatagt gaaagacaat
     1621 cttattgtcg tattcttaat tatttaaaaa gtcaggaggc aatatgaata tcaaagaaat
     1681 aaattgtaaa accaccttga tgcctagcaa attgcccgag gcagattatt gtatcaatcc
     1741 ttacactggc tgcttgcaca agtgtgttta ttgttatgct tgctttatga agcgtttcac
     1801 taatcactct gagaattggg gagaatttct cgatgtgaaa gttaatgccg ctaacattct
     1861 gaatgaagag cttgttaaaa aaagtaaaag aaagggctcg attcttttgg gcagcgtaac
     1921 cgacgtctat caaccggccg agaaaaaatt tcagatgact aggaaaattt tagaagtttt
     1981 agctaaatat gattttcctg tgtcaatcct gacaaaatcg gagttggttg ttagagatat
     2041 taatctacta aaaaagattc ctgattgtga ggtcggtctt acaataacct ctctggacga
     2101 tagaatttcc aaaatctttg aaccgggagc gtctgttccc gtcaaacgat tggatgctct
     2161 aactattttg aaaaacagcg gtcttaaaac ttatgctttt gtagggccaa ttctgccagg
     2221 attaactgat ttaaaattaa ttatcccagc tttgcaagga aaaattgatt ttataatgtt
     2281 tgaatctttg aattatggta aatttaattc ggataacatt aaccaggctt ataatgaagc
     2341 aggggttaat attccagttt ttagggatat agattggagc gaaattgaat cggaggccag
     2401 ggagttaagt caaatttata aaatcacggt caagggattt tataaacact agtatatatt
     2461 ttatgccgtc gttatttaat tataatggcg gttttttatt tactaaatta accttatttg
     2521 ctattttatt agtatgaaag acttcaagag atttttaatt aaagccaaga aaaataccta
     2581 tgccttaagt ggcgagagag gagagagtgt tttagctgat ggcgccaaag aactgaacta
     2641 taaatctgaa aatttttatt atcgcgatag atattatggc tctgatccgt ttgtcggcga
     2701 agaagtagtt tttttaaata ataagccttt ttgggttatg aattattccg gacggtgttt
     2761 agaaactata atatcaaaaa gtgaaattta tagttttctt aaaaaatgtc ttaaaaaggt
     2821 aaacgccaat aatccttttc gcggaccgag tgaatatcaa tcaggtgagt atatctataa
     2881 aaataagact aaaggtgata ttgataattt ttatggagag gagcttatct gttataaaaa
     2941 taaaaaaatt tacgaattaa aatatcatgg tggattagtt gaatagttat ttgtatcttt
     3001 atgttgattt atataaatat ttcagagaaa accatagcgc atgagaaccc tatttattga
     3061 agttttaaaa tatagcagaa aagccttgct gggcttgtta aaatcacggt tccagcccga
     3121 attaccccgt aaattttaaa tagccctaaa caagagctat ttttaaatca atattttatg
     3181 ttatcactta aagtaataga caataaaaaa ttttaactat taccaagaat ctcggatttc
     3241 tagttctaat aatagcaagg gggctaccgt gaacacaaaa taaattccga gaagacaaaa
     3301 attatcggcg ctgcttccat ttctagcaaa gataaaatat cggttttaat tataaaccag
     3361 aaaccaggac agcaaaacaa ctagcgagcg cttatgaaaa tatagaaaaa aatattttaa
     3421 tgatttggga aaagctaagc tacccggaca attaaaaata gacttaagcg gaaagaaaaa
     3481 caaaggggtg gtacggaaaa aataaacgtt ctaacgttag tgataatccc aaaattatac
     3541 caaaatcaaa aaacagagat tatctaaaaa attacttaga aggatgcggg aaaaaaatta
     3601 aaaatagctt tttaatctat aattagtaaa atcttgccaa aagctttatt tttgctataa
     3661 taaatacaga aaacctttga ggttttcatt ttacattaga ctaattatca agaaagatat
     3721 ggaaaataaa ttgaaaccga aaattctggc ggaaattaaa gcggaattat tggcacgcaa
     3781 gcaacaaatt ttagaaagtt tagaagacgt ttcccaaaaa aatccacacg aagcggataa
     3841 tttaggttct aaatttcctg aatacggcga taaacccgat gaaaacgccc aagaaattag
     3901 cgactatgtc gctgatgccg ccgcggaaaa agtcttagaa aaaaccttag ctgatattga
     3961 tagcgcttta aaccgcattg aaaaaggcac atacggcatt tgtaaatact gcggccagcc
     4021 aatttctcct aagcgcttac aggctcgtcc cgtagctagc gcctgtatcg cctgtaaaac
     4081 agaactacaa gaaaatgaat aatttttttc tctcaaaaaa tacatttaaa aacatagcgt
     4141 ggcttctcgt cggcgctatg ttttttatag gcgatcgact tttaaaaaat ttagccttaa
     4201 atataggggc agagcaatat tataatattc tcgacccttg gctaaaattt cgtctagcat
     4261 ttaaccccta tatcgccttt tccctgccca taggcggtca agccctaaac attattatta
     4321 ccactattat agccgctcta gtttttatta tattttattt aatatttaat aaaaataaac
     4381 aaaatggcct aatcataccc ttaacattta ttttatttgg ttccataagt aatctattag
     4441 accgttactt atatggcgct gttgtcgact attttgattt ccgttatttt acaattttta
     4501 atttagccga tattattatt agtattggag cggttagcgc cttaattgtt gcggtaaaaa
     4561 aaaagtaatc tatgtttaat tcattttctt ttgaaaaaat caacaatttt gaaaacaaag
     4621 gctcaatcct ggaatttgaa tattttggtg aaataaataa acgcgcctat agtcaggctg
     4681 ttgatgagta tctaaaccct gaattatata aaataaaaag ccaagaagac gtagaaagag
     4741 tttttttaag aaaaaacaaa gaaaaagaag attacgccaa tagttttaaa aatgattcat
     4801 atataaaatt taaggatgca atggcaatag tggaaaaatg ccaattcgga aatcctgaaa
     4861 tgcccaacgc tatatttgcc agagttttac gcaaagaaat atccaacctg ctgggagatg
     4921 aatacaaagt aaaatttttt accgctgtcg gctctcattt ggacacaaaa catggtgttg
     4981 atgcttttat taaagtatac gataaaaatg agaaagaatt agctcaagtg acactcgaca
     5041 tctccggcag agagaagggg gcatctaagg ccgatctact aattgttgtc agccgagaag
     5101 aacgtgactg ctatgatttt gattctgata attttgacaa ggaaaaattt aataaccgaa
     5161 tcaaaaatga agctttaaga ataaaagaaa ttataactaa aaatagaaaa acttctggag
     5221 agtatccaaa aagcagttac ggacaaaaac aagtcgttaa ataaaaacgc cttaagcaag
     5281 cccttaatgg ccgaaagctg tctgtcaaag aaataaacca caagcacacg gccgagctac
     5341 aaaaaatcgc cattacaggc gagatatcca aaacttttat tttattgtta gctattgtaa
     5401 ataccgcaaa gagcagaaac taacaattga aattaaaaat ctttaaaaga tttccaaatt
     5461 acgaaatctc tttttttatt tctattttcc tctcattctc agaaatacta gtaattttaa
     5521 tagtgataac agaataatct tcccaagctg tccttacttt ctccgcccat tctatcgcac
     5581 ttactacatc ttccctccct agataatcat tagcacctat atctagtaat tccctcccag
     5641 aagataggcg ataagcgtca atgtgacaaa gctgtttaat ctttcccttt ttagccggat
     5701 atagcttcat aatcgtaaaa gtcggactat ttactttggc ttttacaccc agaccgctgg
     5761 ccaaaccttg aacaaagacc gtcttaccga ctcccaaatc gtccgacata gctaaaatct
     5821 ctccaccctg aagagtggtg gctaaattct tacccaaaat aaaagtttct tctgggctat
     5881 ggctaatata tgtttgttta ttccgcataa taacattcta aaggatactc ttgggaaaat
     5941 aaagggtttg tgacaagcac atcagccagt gatataatta gatatatatc taaatatcta
     6001 attatgacgt ttaaccgaac ttctaccgct ctggctgacc ctaaccctca atctattctc
     6061 aatgctttac gaattaaaga gtaagcggta tcagttattg ctgaaaattt aaatataact
     6121 ctcatcacgc tttcacacca ttttgatatt ttaaaaagaa cggggttaat tagtgctcgc
     6181 cgttccggaa gacaaatttt ttatagtctc aatatgatga gcgtcttgga agaaattagc
     6241 caaaccttga ttaagtttat cggacaaaaa ataaaaaata aaaaaatatg gaaaaaatta
     6301 cttttaaaac agaatggcgc gccctcttaa taatcatagc cactatcgcc ttaagctatt
     6361 gggcctatcc acaattaccg acaaccgtgg cctctcattg gaattttcaa ggagaagttg
     6421 acggttggat gagtaaaaat tttcactcta tctttttccc agctctttta gtagtaatgt
     6481 atctaggttt tctaatcatg ccttatatcg acccccaaaa agaacgctat gctgaatttg
     6541 ctaatgtcta tagattaatg cgagatgcca gcctatttat cttacttggc gtctttactg
     6601 tcgccacttt ttataacctc agttattctc ttaatgttag tgctattgtc gctggtctaa
     6661 tcggcgctct catgataata atgggtaatt atttcggcaa aataaaaaga aactggttta
     6721 tcggcatccg cactccctgg tcattatctt cagaaaatgt ctggaacaaa actcaccgtc
     6781 tcggcggtcg cttctttatg gtttggggct tgggcatcgc tatcgctcct tggttagact
     6841 tccgcgtggc gatgattatc ttactagccg gcttagccgc tattatcatt ggtattaatt
     6901 tatattcttt catcctctac cgtcaagaaa agaaaaaata gacacctaaa aacaggcaat
     6961 tgcctgtttt tttgttaggg tggggagcct ggtacgattt aggaacctgg ctcgttaata
     7021 atagttatgt tgatattata gctttagaca aataaaaaag gaagctggtt tagggcttcc
     7081 ttatgaactt tgttcatccc acaaaattgt ttattctaca aaataatgtg gcttgctcct
     7141 gtttctgttc tttttcacta aaattgaatc accttcggac atgttataat aaacaacttc
     7201 cgcaattaaa ttaactgata tagtgtcttg ccctctggta a
//
LOCUS       DAOQSA010000029         6669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_11380_length_6669_cov_10.306320, whole genome shotgun
            sequence.
ACCESSION   DAOQSA010000029 DAOQSA010000000
VERSION     DAOQSA010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6669
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11380_length_6669_cov_10.306320"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PKK65_03435"
     CDS             complement(<1..196)
                     /locus_tag="PKK65_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81755.1"
                     /translation="MIFLLHGADSFRRQQKLNELKQRFMSAIDSLGQSLIILDGRQTS
                     DAELQEKISSGSLFTKKRMVI"
     gene            complement(193..1014)
                     /gene="murI"
                     /locus_tag="PKK65_03440"
     CDS             complement(193..1014)
                     /gene="murI"
                     /locus_tag="PKK65_03440"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNU81756.1"
                     /translation="MLKNKTKSSIGVFDSGLGGLTVLKHLLEKLPPYDYLYLGDTARV
                     PYGGHSQEVIYRYTKEAMDFLFSQGCRLVILACNTASSQALRRLQQEYLPEKYPDRRI
                     LGVIIPLAEYASKSNNIGVIGTRATIASKAYEHEIKKLNPQASVVSNSAPLLVPLIEE
                     GLAGSDVTNKLLKKYLRPLKIKKIKTLILGCTHYSFLLKDIRRIMGNNCRVPDPGEIV
                     ANSLKNYLIKHNNLVSAHKKPGSRHFYLSDKPENFIKLGERFLGQKIDNWKIISL"
     gene            complement(1001..2317)
                     /gene="murD"
                     /locus_tag="PKK65_03445"
     CDS             complement(1001..2317)
                     /gene="murD"
                     /locus_tag="PKK65_03445"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNU81757.1"
                     /translation="MFLNSQNIALLGLGKENQAFLAWIFKHGYKHVVTICDQRTETQL
                     KDIISATPLFKKHLDKLAWRTEANFNKNLDDFEVLFRSPGWPLSCPGVQTALKKGVLV
                     SSAMEYFFAACPTKNTIGVTGSKGKGTTASIIAAILKKAGKKIFLGGNIGIAPFNFFD
                     KIKKNDWVIMELSSFQLEDMHFGPRYAVITNLFKEHLSPADPLNPNYHHSLYAYWQAK
                     LKIAKHPENKLLVANNNLRSRLEKEKLPGKIIYFEASTLPSCLRGIYNQENIGAAVAL
                     SRVLKISAQTYKKAIKNFKNLEHRLEFVGEKNGIKYFDNSFSTTPESTALDLISFQEP
                     IILIAGGADKGADFKPLAKLINKKVKALILLKGTATPRLFVEVKMAGFNKKNIYFADT
                     MAEAISAARKKAIAGDIILLSTACASFGIFKNYKERGDQFKFYAKK"
     gene            complement(2319..5117)
                     /gene="polA"
                     /locus_tag="PKK65_03450"
     CDS             complement(2319..5117)
                     /gene="polA"
                     /locus_tag="PKK65_03450"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNU81758.1"
                     /translation="MQKNLPKFIIIDGNALIHRSFHAIPPTLTTKDGLLVNAVYGFTN
                     FILKAFLELKPEYVVLTLDRPAPTFRHEDYTEYKATRIKAPDELYKQIPLVKEVAIAL
                     DIPIFEKDGFEADDLIGTITKESEKETNWQNIIITGDMDTLQLISDRTSVYTMSRGFS
                     DSLLYGAQEVKDRYGLRPDQIVDYKALRGDASDNIPGVKGIGEKGATELLQAFDTLDG
                     VYKAVAQNNGKIKSRTRELLLQNKDKAYLSQRLATINREVPIKIKWDEFKLDSFDESR
                     AEAVFQKLEFRSLLGKLKQIKNLGQAEQLKKEKTPKVEYQDKFARNQAEKKYQLIADD
                     QSFNIFFKKLKQQNAVAIDTETEGLDPLNAQLLGLSFSWQANEAYYLHLDAKTTTKTD
                     LFNYKKAGAGYDWLKKLQIVLEDEKIKKYGHNLKFDWRVLKNQGILLKGIAFDSLLAS
                     YLLNPDNRQHGLDGVSFRELAWEKISTEELIGKGQEALSFSQVPAEKMAQYAGEDADC
                     AWQLQAILSKKLKKEKLDKLFINLELPLITVLGEMEENGIKLNPKPLAQLAKKLEKRL
                     NNLTKEAYSLGKEEFNLNSTKQLQHILFENLQLDTHGLKKTKTGISTADEELEKLRDQ
                     HEIIALIQEYRELSKLQNTYVLALPKMINPKDGRVHTSYNQSVTATGRLSSTEPNLQN
                     IPTRTEEGREIRAAFVAAEGYSLLSLDYSQIELRLATHLSQDKKMITAFQKNEDIHTA
                     TAAAINGVAWQEVTKEMRRAAKAINFGILYGQGPHGLSQGAGISYSQAKDFIDRYFEV
                     YPGIKKMMDKFIADARNKGYAETLFGRRRPLPDLNADFIQVRHAAERMAINMPIQGTA
                     ADMIKKAMLNIDDYIQDKKNDIRLLLQVHDELIFEIKSDKLEKYIEPLKKLMAEALPL
                     SVPIIVEAASGQNWGELK"
     gene            complement(5216..5386)
                     /locus_tag="PKK65_03455"
     CDS             complement(5216..5386)
                     /locus_tag="PKK65_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81759.1"
                     /translation="MKTKIGIPRVTGNTPEVPELYVGKEFLRQQNNKQKTLKGSKRKQ
                     CGRHRRNLPGGK"
     gene            5499..5603
                     /locus_tag="PKK65_03460"
                     /pseudo
     CDS             5499..5603
                     /locus_tag="PKK65_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492520.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
     gene            5620..6615
                     /locus_tag="PKK65_03465"
     CDS             5620..6615
                     /locus_tag="PKK65_03465"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81760.1"
                     /translation="MMFSFSLIFLAVVFFLILGKGADLLVGNLRAVADRTRIHPVLLG
                     TILGFLTTIPEFMVGLNAIRQGVPAVFLGNIWGGIIVIFGLILGVSIVLQREIKTDGR
                     ILVILPSFIFIIFSLFLGLKGSFHYYDGLLLVVFYLILIYLDYLSGSHYQADGEIIKQ
                     AVSEQQHHHHAWQRFKEKFLFWRRELRREILWALIGLFLVSLSSYVIMEIVGIALGIF
                     PVAPFLVGLLVFAIGTNLPEITVMLRSLKTKSGDLSFGHLVGSAASNILILSLITFIA
                     PIPLSLSWRYYVLILFVVIILSVVSIFYVTTRKFQRWEGVILICLYLSFILFEFL"
BASE COUNT         1672 a         1193 c         1479 g         2325 t
ORIGIN      
        1 caattaccat gcgtttcttg gtgaacaggg agccgctact gattttttct tgcaattcgg
       61 cgtcggaagt ttggcgacca tcaagaataa tcaaactctg ccccagagaa tcaatcgcgg
      121 acatgaagcg ctgttttagt tcatttaatt tttgctggcg acggaaagag tcggcgccat
      181 ggagcaggaa gatcatagag aaattatttt ccagttgtct attttttgac caagaaacct
      241 ttcgcctaat ttaataaaat tttccggctt gtcgcttaaa tagaaatgtc ggctaccggg
      301 ttttttatga gcagatacca ggttgttatg ttttatgaga taatttttta agctattggc
      361 cacaatttct ccaggatcgg ggacgcgaca gttatttccc atgatgcggc gtatgtcttt
      421 taatagaaag gagtaatgag tgcagccgag aattaaagtt ttaatctttt taatctttaa
      481 tggtctcaga tactttttta aaagtttatt cgtgacatcg ctgccagcca aaccttcttc
      541 aattaaagga acaagcaaag gggcgctgtt gctgacaaca gaagcttgcg gatttaattt
      601 tttgatttcg tgttcatagg ccttggaagc gatagtggcg cgcgttccga ttactcctat
      661 attattactt ttggaggcat attctgccaa agggatgata acaccaagaa tgcgtcggtc
      721 gggatatttt tctgggagat attcttgttg taggcggcgt aaagcttggg aagaggcggt
      781 attgcaggct aaaattacta aacggcaacc ttgagagaat agaaaatcca ttgcttcttt
      841 cgtatagcgg taaattactt cttgagaatg tccgccatag ggcacgcggg cggtgtcgcc
      901 aagatagagg tagtcatatg gcggtaattt ttccagaaga tgcttgagta cggttaagcc
      961 ccctagtccg gaatcaaaga cgccgatgct ggattttgtt ttatttttta gcatagaatt
     1021 taaactggtc gcctctttct ttatagtttt taaaaatacc gaagctggcg caggccgtgg
     1081 ataataagat aatatcgccg gcaatagctt tttttctggc cgcgcttatt gcttcggcca
     1141 tagtgtcggc aaaatagata tttttcttgt tgaatccggc catttttacc tccacgaaca
     1201 aacgaggagt cgctgttcct tttagtaaga ttaaggcctt tactttttta ttaataagtt
     1261 tagctagggg tttaaaatca gctcctttgt ccgctccgcc agcaattagg atgattggtt
     1321 cctgaaaaga gattaaatct aaggcagtgc tttctggagt cgtagaaaaa ctgttatcaa
     1381 aatatttaat accgtttttt tctccgacaa attccagccg atgttccaga tttttaaagt
     1441 ttttaatggc ttttttgtat gtttgtgcgg aaatttttaa aactcggctt aaggctaccg
     1501 ccgcaccgat attttcttgg ttgtatatac cccgcaaaca agaaggcaga gttgaagctt
     1561 caaagtaaat tattttaccc ggcaattttt ctttttctaa cctagaacgc aaattattat
     1621 tggctaccag aagtttgttt tccggatgtt tggcaatctt aagtttagcc tgccagtagg
     1681 cgtaaagaga atggtggtaa ttaggattaa gagggtcggc gggtgataaa tgttctttga
     1741 agagattagt aataacggca tatcgtggac cgaagtgcat atcttcaagt tgaaagctag
     1801 ataattccat aatcacccag tcgttttttt ttattttatc aaaaaaattg aaaggggcga
     1861 taccgatatt gcctcctaaa aaaatttttt taccggcctt ttttaaaatg gcagcgataa
     1921 ttgaggcggt tgttcctttg cctttgctgc cggtaactcc aatcgtgttt ttagttggac
     1981 aggcggcaaa aaaatattcc atcgcgctgc ttaccagtac tcctttttta agagccgttt
     2041 gcacgccggg gcaagacagg ggccagccgg gggagcgaaa caaaacttca aagtcatcta
     2101 gatttttgtt aaagtttgct tctgtcctcc aagctaattt atccaagtgt tttttaaata
     2161 aaggagtagc gctgatgata tcttttaact gtgtttctgt tctttggtcg cagatagtaa
     2221 cgacgtgttt atagccatgc ttaaaaatcc aagctaagaa agcttggttt tctttgccta
     2281 gacctaagag ggcgatattt tgcgaattaa gaaacatatt attttaattc tccccaattt
     2341 tggccgctgg ccgcttccac aattatcggt accgataagg gcagagcttc ggccattagt
     2401 tttttgagcg gttcaatata tttttccagc ttatcgcttt taatttcaaa aatcaattca
     2461 tcatgaactt ggagtaaaag gcgaatatca ttttttttgt cctggatata atcgtcaata
     2521 ttaagcatag cttttttaat catatcggcg gctgtccctt ggatcggcat attgatggcc
     2581 atgcgttcgg cggcgtggcg cacctggata aaatctgcgt ttaagtcggg aagagggcgg
     2641 cgacgtccaa aaagagtttc cgcgtagcct ttgtttctgg catcggcaat gaatttatcc
     2701 atcatttttt ttatacccgg ataaacctca aaatagcggt cgataaaatc cttagcttgg
     2761 ctatagctga tgcccgcgcc ctgacttaag ccatgggggc cttggccgta gaggatgcca
     2821 aaattaatag ctttagcggc acgacgcatt tctttggtta cctcttgcca ggcgacgccg
     2881 ttgatagcgg cagcggtagc ggtatggatg tcttcatttt tttggaaggc agttatcatc
     2941 tttttatctt gggaaagatg ggtggcaagg cgaagttcga tttgtgaata atcaaggcta
     3001 agcagagaat aaccttcagc cgccacgaaa gcggctctga tttcccggcc ctcttcggtg
     3061 cgggtgggga tattttgtaa attgggttca gtagaagaga ggcggccggt ggcagtaaca
     3121 ctttggttat agctggtatg gactcgtccg tctttaggat tgatcatttt tggcaaagct
     3181 aaaacatagg tgttttgtag tttgcttaat tcgcgatatt cttggattaa agcgataatc
     3241 tcatgttggt cgcgtaattt ttctaattct tcgtcagcgg tagaaatacc agtcttagtt
     3301 tttttcaaac catgcgtatc cagttgtaag ttttcaaaca aaatatgttg cagctgtttg
     3361 gtggagttga gattaaattc ttccttgcct aaactataag cttctttagt aagattgttt
     3421 aggcgttttt ctaatttttt tgccaattgc gccaaaggtt tagggtttag tttaatacca
     3481 ttttcttcca tctctcctaa gacggtaatc aggggtaatt ctaaattgat aaatagttta
     3541 tctaatttct ctttttttaa ttttttacta aggattgctt gtaattgcca ggcacagtcg
     3601 gcgtcttcgc cggcgtactg cgccattttt tctgcaggaa cctgagagaa ggaaagagcc
     3661 tcctgaccct tgccgattaa ttcttcggtg ctgatttttt cccaagccag ttcgcggaaa
     3721 gaaaccccat ccagtccgtg ctggcgatta tcgggattaa gcagatatga ggcgagcagg
     3781 ctatcaaagg cgattccttt tagtagtatg ccttgatttt ttaaaacgcg ccaatcgaat
     3841 ttcaggttat gtccgtattt ttttattttt tcatcctcta agactatttg caattttttt
     3901 agccagtcat accccgcccc cgctttcttg taattgaata agtcggtttt agttgtagtt
     3961 ttggcgtcga gatgaagata ataggcttcg ttagcttgcc aagaaaaaga taggcccaga
     4021 agctgggcat taagcggatc cagtccctca gtttcggtgt cgatagccac agcgttttgc
     4081 tgttttagtt tcttgaagaa aatattgaag ctttgatcat cggcaattag ttgatatttc
     4141 ttttcagctt gattgcgcgc aaatttatct tgatattcta cctttggcgt cttttccttt
     4201 tttaattgtt cggcttgacc gagatttttt atctgtttta atttaccgag aagggaacgg
     4261 aactccagtt tttgaaaaac agcttccgct cgactttcat caaaagaatc tagtttaaac
     4321 tcatcccatt tgattttaat cggcacttca cggttgattg tagccaggcg ttgactaaga
     4381 taggccttat ctttattttg tagtaataat tccctggttc gtgattttat tttcccatta
     4441 ttttgcgcta ccgctttata gacgccgtcg agggtgtcaa aggcttgcaa taattcggtc
     4501 gcgccttttt cgccgatgcc tttgacgccg ggaatattgt cgctggcatc gccgcgcaaa
     4561 gctttgtaat caactatctg gtcgggtctg agaccataac gatcttttac ttcttgagcg
     4621 ccatagagaa ggctgtcgct gaagccgcgg ctcatggtat agacgctggt tcggtcgctg
     4681 atgagctgga gggtatccat atcgccggtg ataataatgt tttgccaatt cgtttctttt
     4741 tctgattctt tagtaatcgt gccaataagg tcatcggcct caaagccgtc tttttcaaag
     4801 ataggaatat cgagcgcgat ggctacttct ttgactaaag ggatttgttt ataaagctca
     4861 tccggcgctt ttatgcgagt agctttatat tcagtatagt cttcatgtct gaaagtgggg
     4921 gcggggcggt cgagggtaag aacgacatat tctggtttta attctaagaa agccttcaag
     4981 ataaagttgg taaaaccata gacagcatta actagaagtc cgtctttagt ggttaaagtc
     5041 ggggggatgg catgaaaact gcggtgaatt agggcattac catcgataat gatgaatttg
     5101 ggaagatttt tttgcataac tatattttag cacgggcggt agtggataag caaaggtgta
     5161 agagaaataa aaaagcgctc ctttgcgggg gcgcagatta gaggttaatg tttttttatt
     5221 ttccaccagg cagattgcgg cggtggcgtc cgcattgttt cctttttgag ccttttaatg
     5281 ttttttgttt attattttgt tgacgtaaaa attctttacc aacataaagc tcaggcactt
     5341 ccggggtatt tcctgttact ctcggtattc caattttcgt tttcatgttt gatcttttat
     5401 tcgtattata ttaatataaa tagagattaa gtaagtcctt gggattttta ttatagtctg
     5461 ttaaaatatt gatatacagt ctgttggaat attaatataa tgaaattaat actttgccgg
     5521 gatttactga tatttccata tatccgcaat taatgatagc gtcgggctat agctattctt
     5581 tgctgattga taaattaatt tagccgttta ataaatttta tgatgtttag tttttctctt
     5641 atttttttgg ccgttgtttt ttttctgatt cttggtaagg gggctgattt attagtgggt
     5701 aatttaaggg cggtggccga taggactaga attcatccgg tgttattggg gacgatttta
     5761 ggttttttaa ccactattcc tgaatttatg gttggtctta acgccattcg tcaaggagtg
     5821 ccggccgtat ttttaggtaa tatttggggc ggcataattg taatttttgg tttgattttg
     5881 ggcgtgtcaa ttgttttgca aagagaaatt aagaccgatg gccggatatt ggtgattttg
     5941 cccagtttta tatttatcat attctctctc tttttgggat tgaaaggcag tttccattat
     6001 tatgatggct tattgctagt agttttttat ctcatcttaa tttatcttga ttatctatcg
     6061 ggcagtcatt atcaggctga tggcgaaatt attaaacaag ctgtatcgga gcaacaacat
     6121 catcatcatg cttggcagag atttaaagaa aaatttttgt tttggcgtcg ggaattgcgc
     6181 cgggaaattt tgtgggcgct tatcggtcta tttttggtgt cactttcttc atatgtcatt
     6241 atggaaatcg ttggaatcgc gcttggtatt ttccctgttg cccccttttt ggttggtctg
     6301 ctggtttttg ccatcggtac taatttgcca gagataacgg tgatgttgcg ctctttaaaa
     6361 actaagagcg gcgatttgtc tttcggtcat ttagtgggtt ccgctgcaag caatattttg
     6421 attttaagtt taattacctt tattgccccc attcccttat ctttatcgtg gcgctattat
     6481 gttttaatac tttttgttgt tattatttta tcggtggtca gtatttttta tgttacaact
     6541 aggaaattcc aacgttggga gggggtaata ttaatctgtt tgtatttaag ttttattttg
     6601 tttgaatttt tataattatt tttgcttata aataaaaaac tccgtaattg aactgtacgg
     6661 agtttttta
//
LOCUS       DAOQSA010000030         6116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_12959_length_6116_cov_8.388550, whole genome shotgun sequence.
ACCESSION   DAOQSA010000030 DAOQSA010000000
VERSION     DAOQSA010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12959_length_6116_cov_8.388550"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..124)
                     /locus_tag="PKK65_03470"
     tRNA            complement(50..124)
                     /locus_tag="PKK65_03470"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(89..91),aa:Gly,seq:gcc)
     gene            complement(195..278)
                     /locus_tag="PKK65_03475"
     tRNA            complement(195..278)
                     /locus_tag="PKK65_03475"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(242..244),aa:Leu,seq:taa)
     gene            complement(301..688)
                     /gene="ssrA"
                     /locus_tag="PKK65_03480"
     tmRNA           complement(301..688)
                     /gene="ssrA"
                     /locus_tag="PKK65_03480"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(691..1158)
                     /gene="smpB"
                     /locus_tag="PKK65_03485"
     CDS             complement(691..1158)
                     /gene="smpB"
                     /locus_tag="PKK65_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015144213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNU81761.1"
                     /translation="MPILANNRRASFDYDLLENYEAGLVLLGHEAKSAKAGHVNLSGA
                     YVSLRSGNNGSELWLVGAHISPYRFAGKMNDYDPNRERKLLLKKREIAYLIGKIQEKG
                     LTLVPVKMYTKRSFVKLEFALGRGKKKYDKRESIKKRELDRQIRTLTKRQISR"
     gene            complement(1127..2437)
                     /gene="tig"
                     /locus_tag="PKK65_03490"
     CDS             complement(1127..2437)
                     /gene="tig"
                     /locus_tag="PKK65_03490"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNU81762.1"
                     /translation="MQSTKKDLGKSQVELTVSLEFKEFQPYLEEGVRHISEHIKIEGF
                     RPGKAPYERLKQKVGEISILEEAAHIAIRKTIDDVFAKELAGRQPVGQPKVEITKLAP
                     GNPLEYKVTISLLPTVVLGDYKGLNIVKEKPLVSTNDIDKTIKEILEMRAKESLSSES
                     AEEGDKLVLNVSLSLDKVPVEGGQAQDVTVILGKEYMVPGFDDKVRGIKQGEKREFKL
                     LYPETHHQANLAGKMVEFSVTATSIYKREIPALDDELAKAMHFKNVADLKAAIEKNIL
                     ADRERQADIKAELKMLEIIVDKAKFDDIPEDLLEKEADNMMTELERNVTAQGGNFEDY
                     LKHLKKTPTELRLEILPNAVKRIKTALIIREVGIIEKIEVKQKEVEEKLEELKKMYAH
                     DQEVLKQLNSHEYFHHLENMMFNDQVVAQLKSWNYADTGKQSES"
     gene            complement(2625..4637)
                     /locus_tag="PKK65_03495"
     CDS             complement(2625..4637)
                     /locus_tag="PKK65_03495"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNU81763.1"
                     /translation="MTNLLIIASAVAAIIYGFIAGIFILRLPTGNDKMKEIAKAIQTG
                     AKAFLNRQYRSVAIVAAILFLLIGFLPSLGWSTATAFLLGAIFSALTGYIGMLVSVRA
                     NVRTAEAARGGLQKALNVAVRGGSVTGLLVVGLALLGTAGFYFLNPGDLHAIIGFGFG
                     GSLISIFARLGGGIYTKAADVGADLVGKVEANIPEDDPRNPAVIADNVGDNVGDCAGM
                     AADLFETYAVTSIAAMVLGSLLFSGDQNIIIYPLVLGAVSIITSIIGIFFIRLGKKKN
                     IMRALYKGLIATGVLSAIAFYPITLAFIKDAHLAVNIYLASLIGLLVTAALVVITEYY
                     TSTKFAPVKDIAEASKTGHGTNVIAGLAVSMKSTAWPVLIIVAAILGSYMLVGLYGIA
                     VAAVAMLSMAGIIVTIDAYGPITDNAGGIAEMAELGEDVRNITDPLDAVGNTTKAVTK
                     GYAIGSAALAALVLFADFTYKISDLGGATQFLINNPFVLSGLFIGGLLPYLFGAMAMK
                     AVGRAAGSVVEDVRAQFKEKTGIMTGTEKPDYGRTVDIVTKAALKEMILPALIPVVAP
                     IIVGFGFNLFGAGHGIEALGGLLVGTIVTGIFVAVSMTSGGGAWDNAKKYIELGHFGG
                     KGSDAHKAAVTGDTVGDPYKDTAGPAINPMIKVLNIVALLIIGLLL"
     gene            complement(4682..5947)
                     /gene="nusA"
                     /locus_tag="PKK65_03500"
     CDS             complement(4682..5947)
                     /gene="nusA"
                     /locus_tag="PKK65_03500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNU81764.1"
                     /translation="MSELTNAIKQVCEEKNLDYQAVVSTIELALAAAYRKDYGEKNQN
                     IKVVFDPNTGSSKIYDVKTVVDNLPAQEEEEMLRRLNDPDYKREEDKEKRDEMGGDDE
                     EKEHRFNPKTDIQLKEAVIMKHDAQIGEEIMTELPIPESYGRMAAQTAKQVIIQKLRE
                     MERDMILSEFKGKEKEVVGGVVQRREGRLVFVDLGKAIGILPSEEQIFSEHYNPGERI
                     KVYIKEVREGHKGPEVILSRRSEEILKKVFYLEIPEIANGLIEIKGVAREAGSRSKVA
                     IYTETENVDPVGSCIGQRGARIQTIISELGGEKVDIIEYSEDPAKFIANALAPAKILS
                     IELDEAEHKAVVKVSSDKLSLAIGKSGQNVRLAAHLTGWKIDIVEASEDGEKKVADSE
                     EGAAAANEGADSASDSSLDEGQSEPGENK"
BASE COUNT         1841 a         1440 c         1050 g         1785 t
ORIGIN      
        1 agaccaaaag aatataaaga aaaaacagcc cctttttagg ctgttttgtt ggagcgggta
       61 acgagaatcg aactcgtctc tcaaccatgg caaggttgca taatagccac tatacgatac
      121 ccgcaaacat atattttata taacaaatat tcaaatacta agcaaggact ccgcctaaat
      181 tttaaaggga tgcgtgccgc gggcgggaat cgaacccgca ctgtatctct acaactggat
      241 tttaagtcca gcgcgtctac cagttccgcc accgcggcta aataaaaatg tcaacaaaaa
      301 ctgtggagat gatgggagtt gaacccatgt ctaataagtg cccttagata tatttacaag
      361 aatagtccga tttattggag tcgccgacgt tatcggaaac cggacaaaag caacgccggt
      421 cacctctctt tatcttaaaa actccaggag agggctgaaa ttttcaaagc ttcaaattat
      481 gacaccggtt gacggagctg aagcctcccc gcgccgatgg ttacagctat tttttaatta
      541 agccataacg ggagcgaaac tcggaacttt ccaagtaaac gccctgccag tatttttggc
      601 atgtatgttt ttggctattt ttacggttta acctaaaccc gccttgaata tctaaaagca
      661 gatcttatta tcgatgcccg gcatccccga ttatcttgaa atctgcctct ttgttaacgt
      721 ccgtatttgc ctgtctaatt cgcgtttctt tatagactcg cgcttgtcat acttcttttt
      781 tcctctacct aatgcaaatt ctagcttcac aaaactgcgt ttagtataca ttttgaccgg
      841 aaccaatgtc aagccctttt cctgtatttt tccaataagg taagcgattt ctcttttttt
      901 taataacaac ttgcgttctc tgttagggtc atagtcattc atcttaccgg caaaacgata
      961 aggggaaata tgggcgccga ccagccagag ttccgagcca ttatttcctg aacgcaaact
     1021 gacataagcg ccacttaaat ttacatgtcc agccttggct gattttgctt catgtcctaa
     1081 taaaacaaga ccggcctcat aattttctaa aaggtcatag tcaaaactag ctctccgatt
     1141 gtttgccagt atcggcataa ttccaagact ttaactgcgc aacgacttga tcgttgaaca
     1201 tcatattttc taaatggtga aaatattcgt gactgtttaa ttgtttcaaa acctcttgat
     1261 catgagcata catctttttt agttcttcca atttttcttc aacctctttt tgcttaacct
     1321 caattttttc gataataccg acttcacgga taattaaagc agtcttgatg cgtttaacag
     1381 cattaggtaa aatttctaaa cgtagctcgg ttggagtttt cttaagatgt tttaaataat
     1441 cctcaaaatt acctccttgg gcagtaacat tacgctctaa ttccgtcatc atattatcag
     1501 cctctttctc taataaatct tccggaatat catcaaactt ggctttatcg acaattattt
     1561 ctaacatttt aagttcggct ttaatatcgg cttggcgctc tctgtcggct aaaatatttt
     1621 tttctatggc cgctttcaaa tctgccacat ttttaaaatg catcgctttg gccaattcat
     1681 catctaaagc aggaatttct cttttataaa tagaggtagc agtcacggaa aattcaacca
     1741 ttttcccagc taaattggct tggtgatgag tttctggata gagcaactta aattctctct
     1801 tttctccctg tttaataccc ctcaccttat cgtcaaaacc ggggaccata tattctttac
     1861 ctaaaataac ggttacatct tgagcttgac cgccttctac cggcacctta tctaaactta
     1921 gactaacatt caaaactaat ttatcgcctt cttcggctga ttctgaactt aaactttcct
     1981 tagcgcgcat ttctaaaatt tccttaattg tcttgtcaat atcattggta ctaacaagtg
     2041 gtttttcttt tacaatattt aaacccttat agtcacctaa aacaacggtc ggcaaaagag
     2101 aaattgttac cttatattct aaaggattgc caggcgctaa tttggttatt tctacttttg
     2161 gttggccgac tggctggcga cccgctaatt ctttagcaaa aacatcatca atggttttgc
     2221 gaatagcgat atgagccgct tcttctaaaa tactgatttc gccaactttt tgctttaacc
     2281 tttcataagg ggccttaccg ggacgaaaac cttcaatttt tatatgctca gaaatatgcc
     2341 taaccccttc ctcaagataa ggttggaatt ccttaaattc caaagagact gttaattcta
     2401 cctgcgattt tcctaaatct tttttagtgc tttgcataat ctttttgaca attagaagcg
     2461 agctgtggcg ggctcgctaa ttttttcttt tatttattat attttagcag tttttttaga
     2521 aaaagcaagt ttgctttttt gttatttcgg aataattaaa aggctataca aacaaaaaac
     2581 gccagtaata gctggcgctt ttctcattct aaccttatct ttctttaaag caataaacca
     2641 ataatcagca aggcgacaat gttcaaaacc ttaatcatcg gattgatggc cggaccggca
     2701 gtgtccttat agggatcacc aacggtatca ccggtcacag cggctttatg ggcgtcagag
     2761 cctttaccgc cgaaatgacc tagttcaata tattttttag cgttatccca ggcgccaccg
     2821 ccggaagtca tagaaactgc cacaaaaata ccggtaacga ttgtgccgac taatagacca
     2881 cccaaagctt caatgccgtg tccggcgcca aatagattaa aaccgaagcc gacaataatg
     2941 ggagcaacta caggaattaa tgcgggtaaa atcatttctt ttaaggcggc cttagtcaca
     3001 atatcaacgg tacgaccata atcaggtttt tctgtccccg tcataatccc agtcttttct
     3061 ttaaattgag cacgaacgtc ttcaacgacc gaaccagcag cccgaccgac ggctttcatc
     3121 gccatcgcgc caaataaata aggcaataaa ccgccgataa acaatccgga cagcacaaaa
     3181 ggattattaa ttaaaaactg agtcgctcca cccaaatcag aaatcttata agtaaaatct
     3241 gcaaatagta ctaaagccgc caaagcggca gaaccgatag cataaccttt agtgaccgct
     3301 ttggtagtat tacctacagc atctaagggg tcagtaatat tacgcacatc ttcacctaac
     3361 tcagccatct cggcaatacc gccagcgtta tcagtaatcg gaccgtaagc atcaatagtg
     3421 acaataatac cagccattga aagcatagca acggccgcca cggctattcc atataaacca
     3481 accaacatat aggaaccaag aatagcggcg acaataatca aaaccggcca agcggttgat
     3541 ttcatggaga cagctaaacc agcaatcacg ttagtaccat gaccagtctt ggaagcttcg
     3601 gcaatatctt ttaccggagc aaatttggtc gaagtataat attcagtaat caccaccaaa
     3661 gctgccgtta ccaacaaacc gattaagctg gccaaataaa tatttacagc taaatgggca
     3721 tctttaataa aagccaaagt aattggataa aaagcaatcg ccgataaaac gccagtggca
     3781 atcaatccct tataaagagc gcgcataata tttttcttct tgcctaaacg gataaagaaa
     3841 atgccgatga tggaagtgat aatagatacc gctcccaaca ctaaaggata gataataata
     3901 ttctggtcac cggagaatag aagggaacca agaaccatcg cggcaattga ggttaccgca
     3961 taggtctcaa ataaatcagc ggccataccc gcgcaatcgc cgacattatc accgacatta
     4021 tcagcaataa cagccggatt gcgaggatcg tcttcgggaa tattagcttc taccttaccc
     4081 actaaatcag cgccgacatc agccgcttta gtgtagatgc cgcctcctaa acgagcaaaa
     4141 attgaaataa gcgaaccgcc gaaaccaaaa ccgataattg catgcaagtc cccaggattt
     4201 aaaaaataaa aaccagccgt tcctaataaa gccaagccga ctaccaataa gccggtgaca
     4261 ctcccaccgc gaacggcgac attcaaagct ttctgtaaac cgcctcgagc ggcctcagca
     4321 gtgcggacat tagcacgtac actaaccaac atgccaatat aaccggtgag agcggaaaaa
     4381 attgccccca gcaaaaaagc tgtggccgtt gaccagccca aagaaggcaa aaagccgatt
     4441 aacaaaaaga gaatagccgc tactatcgcc acggaacgat actgacggtt taaaaaggcc
     4501 ttagctccgg tttgaatggc tttagcaatt tccttcatct tgtcgttacc ggtcggcaag
     4561 cgaaggataa agatgccagc aataaaacca taaataatag cggccacagc gctggcaata
     4621 attaagagat tcgtcataat attaaatatt aattcttgct cctccaggca agattttaaa
     4681 attatttatt ttctccaggc tcagactggc cttcgtctaa agaagagtca cttgcggaat
     4741 ctgctccttc attagcggcc gcagcgcctt cttcactgtc cgccaccttc ttttcaccgt
     4801 cttcagaggc ctcaacaata tctattttcc aaccagtaag atgagcagct aggcgcacat
     4861 tctggccgct tttaccgata gccaaagaaa gtttatctga gctaacctta actaccgctt
     4921 tatgctccgc ctcatcgagt tcaatgctta aaattttagc cggcgctaaa gcattagcaa
     4981 taaatttggc aggatcttcg ctatattcaa taatatcaac cttttctccc cccaactcac
     5041 taatgattgt ttgaatacga gcgcctctct ggccgatgca ggatccaacc gggtcaacat
     5101 tttctgtttc cgtatatatc gccacttttg accgagaacc agcttcacgt gccactcctt
     5161 taatctcaat taagccatta gcaatttctg gaatttccaa ataaaacact ttttttaaaa
     5221 tttcttcact acgcctagat aaaataactt ccggcccttt atgaccctca cgaacttcct
     5281 tgatataaac cttaatcctt tctccgggat tatagtgttc gctaaaaatc tgttcttcag
     5341 atggtaaaat accgatagct ttaccaagat caacaaaaac caaacgtcct tcgcggcgtt
     5401 gaacaacgcc accgaccact tctttttcct tgcccttaaa ttcactcaga atcatatctc
     5461 tttccatttc tcttagcttc tgaataatta cttgcttagc ggtttgagca gccattcggc
     5521 cataactttc gggaataggt aattcagtca tgatttcttc accgatttga gcgtcatgct
     5581 tcataataac cgcctctttt agctggatat cagttttagg attaaaacga tgttcctttt
     5641 cttcgtcatc gccgcccatc tcatcacgct tttccttatc ttcttctcgt ttataatcag
     5701 gatcattaag acggcggagc atttcttctt cttcctgcgc cggcaaatta tcaaccacag
     5761 ttttaacatc gtaaattttt gaactaccag tgttggggtc aaatacaact ttgatattct
     5821 ggtttttttc tccatagtcc ttacgataag cagccgccaa agctaattcg atggtactta
     5881 caaccgcctg atagtcaagg ttcttttctt cacacacctg cttaatggca tttgttaatt
     5941 cagacataat gtttgttagt ccagccaaca ggctaaagac tcaaaaaata ataaaaacta
     6001 tattcttaaa taatagctct ttacaaagtt agagagaaaa aagctaattg ttcatcacaa
     6061 ttagctttat tttttaaata tagcaaaact tataaatttt gtcaaagtaa tcgaat
//
LOCUS       DAOQSA010000031         5497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_15219_length_5497_cov_6.105476, whole genome shotgun sequence.
ACCESSION   DAOQSA010000031 DAOQSA010000000
VERSION     DAOQSA010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15219_length_5497_cov_6.105476"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(355..786)
                     /locus_tag="PKK65_03505"
     CDS             complement(355..786)
                     /locus_tag="PKK65_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81765.1"
                     /translation="MQHHWSVLCQSSVIDIDSKLLSLYDCLEKINVNTPLKPGMPINL
                     PISFEVVSFLSDTQAKGKRKLELKAELLDPKGQKINQFGGELFFKDGSRRLRSRLKIQ
                     GLTLNDPGLYWLRLHFQGRDGLDFVADCDLPLEVNISYKIL"
     gene            complement(1014..1541)
                     /locus_tag="PKK65_03510"
     CDS             complement(1014..1541)
                     /locus_tag="PKK65_03510"
                     /inference="COORDINATES: protein motif:HMM:NF025084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB domain-containing protein"
                     /protein_id="HNU81766.1"
                     /translation="MSRTNPYKRKQRQANRTLLIFGEGMNEEMFLKHLHKLYCLDTNV
                     AVRIKKGKGGDSVSIVLDADRVPGGFDRRIVLLDNDKAKSEIERARKTARDRGIELIE
                     NTPCLEYLFLSILDSAKDINSAECKKIFEAKYINKKKRRELCEYNKIFPKELLNKKRK
                     TVKTLDYIIKIMEGK"
     gene            complement(1545..2759)
                     /locus_tag="PKK65_03515"
     CDS             complement(1545..2759)
                     /locus_tag="PKK65_03515"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNU81767.1"
                     /translation="MIHSFSCKNFYSFKNLTTVDFKVNEKAPNNDAYFKADSGWRLSK
                     IETVIGPNASGKTNLLKVLPFLKWLIVNSFNTSPDAPLPIKPFLFGANKNKPVELSVD
                     FEINGHIFVYTFVLDESKIILEELKIKNKSIEKVTAKKVFSRRWHEEIKAYELDDKKF
                     NLPKGIKDSLRLNASVISVAARFNHKNSLEVFNFWKKVETNVVEAGWVGDRLLPNSGQ
                     QLVEALDFFSEKENIHIKNEAEKLLSQFDLGLDSLEIVKEKKDNSLNIKVRMAHTFGG
                     DKEYLPMQYESSGTKQLLVLLQTILLVLANGGVAILDEFDVNLHPEIVSALFDLFLQV
                     ETNPKNAQLLFSSHSHLILSKLDKYQIILTEKNKKGESEAWRLDEVTGVRADDNYYAK
                     YMAGAYGAFPRL"
     gene            complement(2948..3790)
                     /locus_tag="PKK65_03520"
     CDS             complement(2948..3790)
                     /locus_tag="PKK65_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRO family protein"
                     /protein_id="HNU81768.1"
                     /translation="MSKESKNKALAIFAGKQIRRHWDEEKELWYFAVVDVIASLTNSD
                     NPQVYWRVLKKRLTDEGANETVTKCNALKMLAPDGKMRLTDAADTETLLRLIQSIPSP
                     KTEPFKLWLARVGYERLEETSDPELAINRALKTYFRKGYSLEWINQRLKSIEIRKALT
                     DEWEKRGVKEGLEFAILTDEITKAWAEMSTKEYKKLKNLKKENLRDNMTNLELVLNML
                     AETATTEISQAVEPKDFEENKTVAQEGGGVAGNARREIESKTGKKVISRKNAKILSNN
                     KKLK"
     gene            complement(4097..4708)
                     /gene="lexA"
                     /locus_tag="PKK65_03525"
     CDS             complement(4097..4708)
                     /gene="lexA"
                     /locus_tag="PKK65_03525"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNU81769.1"
                     /translation="MSILTKKQKEVFDFISEYESEKGYAPALNEIKEAIGVAAVSTVH
                     EHVSNLIKKGVLKRVKGAARSLELAKVKFGASLSVPLVGVIACGEPIEAIEVPDAVVQ
                     IPADGRFQAGELYALRAKGDSMIGDGIFSGDFLIIKKQSWANNGETVVAIIDDNEATL
                     KRFYKEKDKIRLQPANTDYEPIYRRQVDIRGIVVKVIRDLVPA"
     gene            complement(4911..5339)
                     /locus_tag="PKK65_03530"
     CDS             complement(4911..5339)
                     /locus_tag="PKK65_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81770.1"
                     /translation="MQNVWSVVCKNSIIDGETNSLSLLETLDEITINYREGIDSHNVK
                     LLPVSFHIVTLWFDENVKKDRKFILSIDIIDPKGKVLNTFNQDCVIPTGSVRLRATSK
                     INGFSFTDEGKYLVVVKYKQTGKFIQVAEIPVSIHFKKEA"
BASE COUNT         1701 a         1168 c          821 g         1807 t
ORIGIN      
        1 agttgttgat ttggccacag tagaaaaaag tataatatta cacccctttc tactgcggcc
       61 aatgatttta aggttctatt tttacaaagt tattatatca ttttaattat attctgttaa
      121 tggttgccct aggaaaaaga ccttaacatt taagacagtt taatactcaa tttagcccct
      181 aggaccaaag cgactttagc caatcattta atgctaaaat tttaggcacc tttccctccg
      241 ggcgacatta ctctgatcct gtcaattttt tagctacagt tgtttgactc attgccgtcc
      301 atctgcgtca aataaagcct gattgtgggt tttaatggga gctaaacgga taatttaaag
      361 aatcttgtaa ctaatattga cttccagggg taaatcacaa tcggccacaa aatcaagacc
      421 gtccctacct tgaaaatgga ggcgtagcca atagagccca gggtcattta gagttagacc
      481 ttggattttt aaacggctgc gcaaacgccg agagccatcc ttaaaaaata attcgcctcc
      541 gaattgattt atcttctgac ctttaggatc aagcaattca gcttttaatt ctaatttgcg
      601 cttgcccttt gcctgggtat cgcttaaaaa gctcaccacc tcaaaggata tgggtaaatt
      661 aatgggcatg cccggcttta agggggtatt gacattaatt ttctccaagc aatcatacaa
      721 actaagcagc ttgctatcga tatcaataac tgagctttga cacaaaacag accaatgatg
      781 ttgcatatga ttattgatgt ttattattta gaaaagctaa aactagtatt ccggtttaac
      841 ctcttgcttc gcttaataat gtgtatccac ccactaacct gagccgaggg attaaattta
      901 aaaccaggta aattaaaatt aaatattttt accaattatc ggcactaaaa tttaaagcgg
      961 aggaaaatct gtttaataaa cttaaaccca cattaatatt acccttctcc aaattacttg
     1021 ccctccatta tcttaataat ataatcaagt gttttgactg ttttcctttt tttattcaat
     1081 aattctttcg gaaaaatttt attatattca caaagctctc ttcttttctt cttatttata
     1141 tattttgctt caaatatttt tttacattcc gccgaattta tatcttttgc agagtctaaa
     1201 atagataaaa aaagatactc cagacaaggc gtattttcta ttaactcaat tcccctgtct
     1261 ctagccgttt ttcttgcccg ctctatttca ctcttcgctt tatcattatc tagaaggact
     1321 attcttcgat cgaacccacc cggcacacga tcagcatcaa gaacaatact aactgagtca
     1381 ccgccttttc ccttcttgat acgaacggca acattcgtat caagacaata taatttatgt
     1441 aaatgcttta aaaacatttc ctcattcatc ccttcaccaa agattaacaa agttctattt
     1501 gcttgacgtt gttttctctt gtagggattg gttctagaca taaattagag tcgaggaaaa
     1561 gcgccataag ccccagccat atatttagca taataattat catccgccct tactccagtc
     1621 acttcgtcca aacgccaagc ctcactttct ccttttttat ttttttcagt caaaataatc
     1681 tggtatttat ctaatttgct caaaattaaa tgactatggc tactaaataa caactgggca
     1741 ttcttggggt ttgtttcaac ttgtaaaaat aaatcaaata aagccgagac tatctctgga
     1801 tgtaaattaa catcaaattc atccaatatc gctaccccgc cattagctaa aactaataaa
     1861 atagtctgca gtaataccaa aagctgttta gtgccagatg actcatattg cataggaaga
     1921 tattccttgt ctccaccgaa agtatgggcc atccttactt tgatgtttaa actattatcc
     1981 tttttttctt taacaatctc taacgaatca agcccaagat caaattgtga taacaacttc
     2041 tctgcctcat ttttaatatg aatattttct ttctcactaa aaaaatccaa ggcctctact
     2101 aattgctgcc cggaattcgg caacaatcga tctccaaccc aaccagcctc aacgacatta
     2161 gtttctacct ttttccaaaa attaaagact tctaggctat ttttatgatt aaagcgagca
     2221 gcgacactaa taacactggc gtttaaacgc aaagaatctt taatcccctt gggtaaattg
     2281 aatttcttgt catctaactc ataggccttt atttcttcat gccaacgacg agaaaaaact
     2341 ttcttggcag tcactttctc aatgctctta ttttttattt ttaactcttc taaaataatt
     2401 ttactctcat ctaagacgaa ggtataaaca aaaatatggc cattaatttc aaaatcaacc
     2461 gataactcta ccggcttatt cttgttcgct ccaaataaga aaggcttaat cggcaaaggg
     2521 gcgtccggag aagtattaaa agaattgaca attaaccatt ttaaaaaagg caaaaccttt
     2581 aataaattag tttttcctga agcgttcgga ccaataaccg tttcgatctt agagaggcgc
     2641 catccagaat cagctttaaa ataagcatca ttatttggag ccttctcatt aaccttaaaa
     2701 tctaccgtcg ttaaattttt aaatgaataa aaatttttac acgaaaatga atggatcata
     2761 catttaatat attttaattg attccactca aatgatagta aaaataaata tatatgtcaa
     2821 taatctacaa atatttgtat gatatataat aatagctgtt attttacccc ccttttaccc
     2881 ctaaagtttt atttattaaa atactatttc acaaacttta cttaattaat attaaatatt
     2941 atattaatta ctttaatttc ttattattcg atagtatttt agcatttttg cgactaatta
     3001 ccttcttgcc ggtctttgac tcaatttcac gcctagcatt accggccaca ccgccaccct
     3061 cttgggctac tgtcttattt tcttcaaaat ccttcggttc aactgcttga gatatttcgg
     3121 tggtagcagt ctccgctagc atatttaaaa ctaattctag attagtcata ttatcgcgca
     3181 aattttcctt ctttaaattt ttcagtttct tatattcttt ggtggacatt tctgcccaag
     3241 ccttagtaat ttcatcggtt aaaatagcaa actccaaacc ttctttaacg cctcgctttt
     3301 cccactcgtc agtcaaagct ttgcgaatct ctatactttt aagtctttga tttatccatt
     3361 ccaaactata gcctttccgg aaataagttt taagggcacg attaatggcc aattccggat
     3421 cggatgtttc ttccaacctc tcataaccga cgcgagccaa ccaaagctta aagggctctg
     3481 tcttaggaga ggggatggat tgaataaggc gaagcaaggt ttctgtgtca gcggcatctg
     3541 taagtcgcat tttgccatca ggcgccaaca ttttcaaagc gttacatttt gtaacggttt
     3601 cgtttgcgcc ttcatcagta agacgttttt taagaacacg ccaataaacc tgtggattat
     3661 cactgtttgt tagcgaggcg attacatcaa ccaccgcaaa ataccacagc tctttttctt
     3721 catcccagtg gcggcggatt tgcttgcctg caaaaatggc aagcgcttta tttttagatt
     3781 ctttggacat aaatttatta agctaatttt aataaattaa aaacatttca ctcctacctc
     3841 ttacattcta gcataaatca cccgaagcca gcttaaagta taaataaaaa cccgccacga
     3901 ggacggattt tagaagaagg gcttatctcg cccaaattag gacctgccgg gactgcatca
     3961 gcttattgta cttggagcgc agcaagcggc gatcacgggg tgaatctttg ctgacaatgt
     4021 ttatcagctt tttttgagtg gagaagattg aagtcatcat atgtttaaaa ttaataaaaa
     4081 taaataagtt aattaattaa gcggggacaa gatcgcggat aactttgacg acaatacctc
     4141 ttatatccac ctgacgacga taaatcggtt cataatcggt attggccggc tgtagtctaa
     4201 ttttatcctt ctctttataa aatcttttta aagtcgcttc gttatcatca ataattgcca
     4261 ccaccgtctc gccgttattg gcccaagact gtttcttgat aatcaggaaa tcgccgctga
     4321 agatgccgtc cccaatcatg gaatcgcctt tcgctcttaa agcatataat tctccggcct
     4381 ggaaacgtcc gtccgcggga atctgaacca cggcatctgg cacctcaatc gcttcaatcg
     4441 gttcaccaca ggcaatcacg ccgactaaag gcacggatag actggcgcca aattttacct
     4501 ttgccagctc taaactccgc gccgcgccct tcactctttt taaaactccc ttcttaatta
     4561 aattagaaac atgttcatga acggtagaaa cagcggcaac gccgatggct tctttaatct
     4621 cattgagcgc cggcgcataa cccttctcgc tttcgtattc ggaaataaag tcaaagactt
     4681 ctttttgttt tttcgttaaa atactcatat ttactgcctt atttattgat attatcatta
     4741 tacccgaaac taaaccgaaa ggcaagagct tggctgtgga taagtaaaaa gaccgttttt
     4801 gcaaataaaa aagccgccga gatatcaagc ggcatttttt aaaacgccaa ttattataac
     4861 ggatgacatt tttatatcaa aaccctttaa cttatcggct gctaactaag ctaggcttct
     4921 ttcttaaaat gaatacttac cggaatttcg gcaacctgaa taaattttcc cgtctgctta
     4981 tatttcacaa ccaccaaata ctttccttca tcggtaaaac taaagccgtt aatttttgaa
     5041 gtggcgcgaa gtctcacgct gcccgttggt ataacacaat cctgattaaa agtgtttaaa
     5101 accttacctt taggatcaat tatatcaatc gataagataa atttgcgatc ttttttaaca
     5161 ttttcgtcga accaaagggt aacaatatga aatgataccg gcaataattt aacattatgc
     5221 gaatctatcc cttccctata attaattgta atttcatcta atgtttccag taagcttaaa
     5281 gagttagttt caccgtcaat tattgaattt ttacacacaa cagaccaaac attttgcata
     5341 ataatattat tattttagaa taattattta tatctaccag atattataaa catctacata
     5401 aattatcact ggataaatct tcaaccttaa ccgtaaaagc ggtattatta gacagagaaa
     5461 catctttatc caatatctgc ctatccccga cttgaat
//
LOCUS       DAOQSA010000032         4975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_17694_length_4975_cov_12.244309, whole genome shotgun
            sequence.
ACCESSION   DAOQSA010000032 DAOQSA010000000
VERSION     DAOQSA010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17694_length_4975_cov_12.244309"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..1192
                     /locus_tag="PKK65_03535"
     CDS             71..1192
                     /locus_tag="PKK65_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006849436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNU81771.1"
                     /translation="MINREITKNIQDFLFKGKIVIIYGPRQSGKTTLVKSIMSQYLND
                     YVYLNCDEVDVRLALSNKTSTELRSFIGAKRLVVIDEAQRVENISLTLKLLADNFPET
                     QVLATGSSAFELSNRISEPLTGRKKEFFLFPFSVGELVAAFSVREVQRRLDDFLIFGL
                     YPEVVNSGADRAGIVRELATSYSYKDVLAYQDIRRPELLEKLLQALALQIGNEVSYTE
                     LAGLLGVNKVTVENYVRILEQAYIIFRVAPFSRNLRNELKKKRKIYFYDLGLRNALIN
                     NFNPLSLRQDVGALWENFLVAERFKRMRNAGLFNNIYFWRTTAGKEIDYLEENNGVLS
                     GYEFKWNRANFSAPKEFLSNYKGSFVKLINRENYLDFIV"
     gene            1393..1638
                     /locus_tag="PKK65_03540"
     CDS             1393..1638
                     /locus_tag="PKK65_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81772.1"
                     /translation="MSLLVVLGTLSIAYLGFAFIAFAALLDNGGTGDIGDILKWMRNP
                     YFTGIIIVSSILLGFLKKTRKRLKNLIADLAENFFSD"
     gene            complement(1734..1886)
                     /locus_tag="PKK65_03545"
     CDS             complement(1734..1886)
                     /locus_tag="PKK65_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81773.1"
                     /translation="MDEKNERIVPPILELREIKMNAEEKKKILHRFSKEKRKKRERKI
                     KRPMRR"
     gene            complement(2058..4127)
                     /gene="recG"
                     /locus_tag="PKK65_03550"
     CDS             complement(2058..4127)
                     /gene="recG"
                     /locus_tag="PKK65_03550"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008165.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNU81774.1"
                     /translation="MLLSVPLPALNRVGKNTAQSLKKLGLETVQDLLLYFPFRYDDFS
                     QTTSIDKIEAGQNISITGTIELIQNKRSVKQRRQLTEALISDDSGLIKVIWFNQPFIA
                     QNLKPGDRVSLAGKTAESYGQLTLISPQYEKIGANGVLIHTQGLVPIYHLNADITQKQ
                     FRFLIKQAIEAADKFKEWLPESVRKELQLLPLATAIKRAHFPENMEQALAARKRLGFD
                     ELFIRQLRSQALKYQLKNKVAPKIIFQEMMTKKFVQSLPFTLTTGQKQAAWEILQDIN
                     KDAPMSRLLEGDAGSGKTVVAAMALFNVAINKYQGALMAPTEILAGQHYQSIQKLLGA
                     FNVKIALLTGHQAEANFELPKNKAERREKIIKDAEIIIGTQALIQDYNISKLALVIVD
                     EQHRFGVKQRQKLQQSGGGLWPHFLSMTATPIPRSLALSLYGDLDLSLIKELPKNRKP
                     IITKVITEEQRDQAYNFVRQQIIAGRQAFIICPLVEESDKLGVRSATAEYKRLKKEVF
                     PDLEIGLIHGKLKSAEKEKIMSALANKDIQILVATAVVEVGVDIPNASVILIEGAERF
                     GLAQLHQFRGRVGRGEEQSYCLLLTSKEQQNPKTAERLQALSKYNDGLSLAKIDLKLR
                     GAGDLYGLVQSGFPELRIASLFDYEMIKKTQISAEKIIKSDPELANYPALREKLGDTS
                     NEEAHLE"
     gene            complement(4178..4249)
                     /locus_tag="PKK65_03555"
     tRNA            complement(4178..4249)
                     /locus_tag="PKK65_03555"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4214..4216),aa:Arg,seq:gcg)
     gene            complement(4283..4807)
                     /locus_tag="PKK65_03560"
     CDS             complement(4283..4807)
                     /locus_tag="PKK65_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81775.1"
                     /translation="MDNQETSKNFIQWQVPEYKKPKRSKRWYLLAAIFVFICLFFSFF
                     TISDWHIVFLGARSNFLFALIILISMAITIIHDSQEPKMVTIKLGPEGIQIGRRFYDY
                     DEIKQFCVLYKPKQSIKNLYLEFNNKMLPRLSIPLRRQDPLNVRNYLVRYLNEDLERI
                     APPLSEELTKLLKL"
     gene            4880..4973
                     /locus_tag="PKK65_03565"
     tRNA            4880..4973
                     /locus_tag="PKK65_03565"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4914..4916,aa:Ser,seq:gct)
BASE COUNT         1372 a          862 c         1048 g         1693 t
ORIGIN      
        1 tttaatgacc taaataatat ataattaggt tattataatg acctaaataa tataattata
       61 aatattagat atgattaatc gagaaattac caaaaatatt caggactttt tatttaaggg
      121 taagattgtt attatctatg gtcccaggca atcaggtaag actactttag taaaaagtat
      181 aatgtctcaa tatcttaacg attatgttta tttaaattgt gatgaggttg acgttcgcct
      241 ggctttaagc aataaaactt ctactgaatt acggtctttt atcggcgcta aacgtctggt
      301 ggtaattgac gaagctcaga gagttgaaaa tattagcctg actcttaagt tgttggccga
      361 taattttccg gaaacacagg tactggcgac gggatcttct gcttttgaat tatctaatcg
      421 aatttcagaa cctttaactg ggagaaaaaa agaatttttt ttatttccat tttctgtggg
      481 agaattggta gcggcctttt ctgtgcgcga agtgcagcgc cgtttagatg attttttaat
      541 ttttggctta tatccggagg tagttaattc gggggcagat agagccggaa ttgtacgtga
      601 attggctacc agttattctt ataaagatgt tttagcttat caagatattc gccgtccgga
      661 acttttagaa aaattattac aggctttggc tttgcaaata ggcaatgagg tttcttatac
      721 tgaacttgct ggtttactgg gggttaataa agtaacggtt gagaattatg ttcgtattct
      781 tgagcaggcc tatattattt ttcgggtagc ccccttttcc cgtaatttgc gcaacgaatt
      841 aaaaaagaag cgtaaaattt atttttatga tttaggccta cgcaacgctt taattaataa
      901 ttttaatcct ttatctttgc gtcaagatgt gggcgcgctt tgggaaaatt ttttggtagc
      961 ggagcgcttt aagcgtatgc gtaacgccgg tctgtttaat aatatttatt tttggcgaac
     1021 tacggccggc aaggaaattg actatcttga agagaataac ggagttttgt ccggctatga
     1081 gtttaagtgg aatcgggcta acttttctgc tcctaaagaa tttttatcta attataaagg
     1141 aagttttgtt aaattaatta atcgggaaaa ttatttagac ttcattgttt agtaaaacta
     1201 ccacgttacg ctactattgc gtaaactatt gacaataatt tataaaagtg atatagttca
     1261 gttatcgtac cttgaaaaag aaggtttaaa agtttgtaag gattgatcaa ttctggccag
     1321 gccttataaa cttttaaacc aaacaaaata atgaaaacaa aaaaaagagt tcttttttta
     1381 aaaaggtttt ttttgagctt gttggttgtt ttggggacgt taagcatcgc ttatttaggc
     1441 tttgctttta tcgcttttgc cgccctgctt gataacggcg gtactggtga catcggcgac
     1501 atccttaaat ggatgcgtaa cccctatttt accgggataa ttattgtttc ttcaattctt
     1561 ctgggttttt taaaaaaaac cagaaaaaga ttgaagaatc taatagccga cctggcagaa
     1621 aattttttct ctgattagaa ggatgcctcc cgtggtaaag ggaggctttt tatttggttg
     1681 atataagaaa aacctccgtt tgaagtggag gtttttatat tttttacaag gccttatcgc
     1741 cgcatcggtc gtttgatttt tctttctctt ttttttcttt tttctttgga aaaacgatgg
     1801 aggattttct ttttttcctc ggcattcatc ttaatctctc taagctcaag aattggcggc
     1861 acaattcttt catttttttc gtccatgttt ttagcgtttc ttacaaacac ttctcctgct
     1921 tttgaaaaaa tatttttatt ggagaagtaa attgggcaac cattaaaaca agctttgtaa
     1981 gtattcattt ttcttaggtt ttatgaaatg aactataaat attagattat atatttttat
     2041 tttttttgtc aataacgtta ttctaaatga gcctcttcgt tactggtatc gcctaatttt
     2101 tctcttaaag ctggataatt agctaattct gggtcagatt taataatttt ttctgcactt
     2161 atttgggtct ttttaatcat ttcataatca aataaagaag caattcttag ttcagggaat
     2221 ccgctttgga cgagcccata taaatcgcca gctccacgta atttcaagtc aattttagcc
     2281 agagataggc cgtcattata tttacttaaa gcttgtagcc tttccgctgt tttgggattt
     2341 tgctgttcct tactggttaa cagcagacag tatgattgct cttcgccccg gccgacacgg
     2401 ccgcggaatt gatgtaattg ggcgagcccg aagcgttcgg ctccttcaat caggatcaca
     2461 ctggcattag gaatgtccac accaacctcg accaccgcgg tggcaacgag tatttgtatg
     2521 tctttattag ccaaagcgct cattattttt tctttctccg cgctttttaa tttaccatga
     2581 atcaaaccga tttctaaatc gggaaatact tcttttttta ggcgtttata ttcagcggtc
     2641 gctgacctta cccctaactt atccgattct tctactaagg ggcagataat gaaagcttgg
     2701 cggccggcga tgatttgctg gcgcacaaaa ttataggctt gatcgcgctg ttcttcggta
     2761 attactttag tgataatagg tttacgattt ttgggtaact ctttgattaa agataaatct
     2821 agatcgccgt aaagggagag cgccaaagag cgaggtatgg gggtggcggt cattgacagg
     2881 aaatgcggcc agaggccgcc gccgctttgt tgcagttttt gtctttgctt gacgccgaag
     2941 cgatgctgtt cgtcaacaat tacgagagct aatttagaaa tattataatc ttggatcaag
     3001 gcttgagtcc cgataataat ttccgcgtct ttaataattt tctctcggcg ttcggcttta
     3061 tttttgggca attcaaaatt agcctcggct tgatgaccgg tgagcagagc aattttgaca
     3121 ttaaaagcgc ctaatagttt ttgaatactt tgataatgtt gtccggctaa aatttcggtc
     3181 ggcgccatta gggctccttg gtatttatta atagcgacat taaataaagc catcgccgct
     3241 actacagttt taccggagcc agcatcacct tcaagcaggc gcgacatggg cgcgtcttta
     3301 ttaatatctt gaagaatttc ccaagccgct tgtttttggc cggtggtgag agtaaaggga
     3361 agcgattgaa caaatttttt ggtcatcatt tcttggaaaa taattttggg agcgacttta
     3421 ttttttaatt gatattttaa ggcctgagaa cgcaattgac ggataaagag ttcgtcaaag
     3481 cctaaccgtt tacgggccgc cagagcttgt tccatgtttt cgggaaagtg agcccgcttt
     3541 atcgctgttg ccagaggtaa aagttgtaat tcttttctga cactttccgg caaccattct
     3601 ttaaatttgt cggccgcctc tattgcttgt ttaattaaga agcggaattg cttttgggtg
     3661 atgtcggcgt tgaggtggta aatgggcact aaaccttgag tgtgaattag aactccattc
     3721 gcccctattt tttcatactg cggggagata agagttagtt ggccataact ttcggccgtt
     3781 tttcccgcta aagacactct gtctcctggt ttaagatttt gggcgataaa tggctgattg
     3841 aaccagataa ctttaataag accactatca tcactgatta gagcctcggt taattgtcgt
     3901 ctttgtttga cgctgcgctt gttctgaatc aattctattg ttccggtaat actaatattt
     3961 tgtccggctt cgatcttatc tatagaggtt gtttggctga aatcgtcata gcggaaagga
     4021 aaatataaaa gcaagtcttg gaccgtttcc aatcctagtt tttttaaaga ttgagctgta
     4081 tttttaccga cgcggtttaa ggccggtagc gggacgctaa ggagcatttt ataaaaatag
     4141 gcgccttaat aggtgcccgt aagataataa attagtgtgc cctcaccagg aatcgaacct
     4201 ggattacaag atccgcaatc ttgcgtttta tccattaaac tatgggggct ggaaattcta
     4261 atttcatgat aggcgaatta tactatagct tgagtaattt cgtcaattct tccgacaggg
     4321 gaggggcaat tctttccaaa tcctcgttaa gataacggac aagatagttg cggacgttta
     4381 aggggtcttg gcggcgcaga gggatagata ggcgggggag cattttgttg ttaaattcca
     4441 gataaagatt tttaatgctt tgttttggtt tatagagaac gcaaaattgc ttaatttcat
     4501 cgtaatcata gaaacggcga ccgatttgaa ttccttcagg tccaagcttg attgtgacca
     4561 tttttggttc ctggctgtca tggattatcg tgatggccat ggaaataagg ataattaagg
     4621 caaaaaggaa gttagacctt gcccctaaaa agacgatgtg ccagtcggaa atagtgaaaa
     4681 atgagaaaaa aagacaaata aaaacaaaaa tagcggccag tagataccag cgcttacttc
     4741 ttttcggttt tttatattcc ggcacctgcc attgaatgaa atttttgctt gtttcttggt
     4801 tgtccatatt gtttgacaat aaataagaga ttttatatag taattatacc ataaaattta
     4861 aaagaaaaaa atattattag gagaggtggc tgagcggctg aaagcggcag gttgctaacc
     4921 tgttatagga gtttttgctt ctatccgggg ttcgaatccc cgcctctccg ccaat
//
LOCUS       DAOQSA010000033         4929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_17926_length_4929_cov_6.846943, whole genome shotgun sequence.
ACCESSION   DAOQSA010000033 DAOQSA010000000
VERSION     DAOQSA010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17926_length_4929_cov_6.846943"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(279..1853)
                     /locus_tag="PKK65_03570"
     CDS             complement(279..1853)
                     /locus_tag="PKK65_03570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNU81776.1"
                     /translation="MRISKLLHKHYFFLALILVLAVCLRLPNLSQPFWGDEILSLQIV
                     RHFQDNISGLFQYLRAVEVHPPLYYLILIGWTKLFGFSEMAVRSLSLIFGLATVALSY
                     FLGKQLWKNARAGLLAALLVAVMPMPIIFSTEARPYIIFSFLGLLCLYKLNTYLDKKH
                     WSQLVYFSIFAIFGLYLHYSFLLILFPLLAWWLFKIIIDKRREDLFRLVLVLASIFLG
                     FYWWLPAMLYKVLLGNYELMNLPRTIFSHRALYFFEASFNQLIWTVKDKQVLPLEILV
                     ILVSKLAAAAALLWLLLKKEKEIIFPFLHISFLVIISLVIFLFSPASENYVSIFEKHV
                     FWLSILLALALAGLASRLKTKAAAALISIFIVSFITFDAKILLSQNSFDADRAQKLIA
                     EQINDGFLPGDIVIDNFSYDRSNLNYYLHPHISAYGFYPPQLFDWQNDIYASRNTLGL
                     LENDAQSRIWPVSGEALDLKMNYIIRQTSPTRIWLAYADYQDYGLKRWLEANGWRHAI
                     FSIDRFSPLDLYVKKQ"
     gene            2256..2996
                     /locus_tag="PKK65_03575"
     CDS             2256..2996
                     /locus_tag="PKK65_03575"
                     /inference="COORDINATES: protein motif:HMM:NF014080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNU81777.1"
                     /translation="MDITILKKLGLSDKEMTIYLKLLEGGPISVRRLAEKAKLNRGIV
                     YEVLKTLQEKGLASYYNDTTKQKFVAENPEKLRRLADIEVDRVKNLQNNINSLILELK
                     SIRGQADNQPVTKLYEEDAGLRTILNDLLDTLKDKEEKEYYVYSAKDSSSDIGRAFPG
                     FNKERKKRNIYVKAISLADGGGTHGLDERRWLGTNDCSATFIIIYAGKCAFISRDKQG
                     SPVGVLIENQMIYNTQKTIFLNLWKRLA"
     gene            3001..4839
                     /gene="glmS"
                     /locus_tag="PKK65_03580"
     CDS             3001..4839
                     /gene="glmS"
                     /locus_tag="PKK65_03580"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNU81778.1"
                     /translation="MCGIASYVGHKNAYPLILEMLKRLEYRGYDSAGIALYSHGKMKI
                     YKEQGAITALAELLSKQKPSGSLGIGHTRWATHGSATKLNAHPHHSQNNTLSIVHNGT
                     ILNYYKLKEELEKANYKFYGETDTEILVNYLDYRQQQSKSSLLEVITDVAPNLEGRSV
                     FVIHNSQSPNNLYACNLGGELFIGRSQEGEYFICSDKNALAGYADEYFALENRQIAIV
                     QKEKNCRLLNIISAEELPCPWEQLKINFETLEKGSYPHFMLKEIFEQEKVVAKTIDSL
                     IADNGQSLNLMFNHWRQKIKKINKLVIIACGTSWHAGLVAKYFFAELAKLPVYCEYAS
                     EFLSESVGAEDIVIAISQSGSTADTLQAVQAAKEQGASVISICNVDGSTLTRNADVSL
                     LTKAGPEIGVASTKAFTTQLVILYILSTKFGRWRNRLNEAEEKKLWRELKTIPKKIKE
                     ILLSSPEIERLAKKYKDYPNFLFLGRGQNFPIALEGALKLKEVSYIHAEGLSAGEMKH
                     GSIALIDDQMPSFFIAVKDKQYDRVINNLLEIGGRKGRIIAVTTEKDQVIIKRTDDII
                     SIPPCPPLLYPFLTVIPTQLFAYYVARERGTNIDKPRNLAKAVTVE"
BASE COUNT         1782 a          970 c          873 g         1304 t
ORIGIN      
        1 tagacaagaa aataagactg acggagaatg gcaacatgtg aatattgacg attataataa
       61 gatatctcga gacaatctag ataatttcga taaaataaaa ggtaaaatag tttataataa
      121 cgataagtcc ggaactcttg taaaatcatt aaaacctacc gagccttgac ggactcggta
      181 ttttaccact ctagctctct tccgaggcct aaccccggtt tccccggtag agcacaaagt
      241 aaaagacgcc aattaggcgt cttttttaat atataatatt actgtttctt cacatacaaa
      301 tccaggggag aaaaccgatc tatagaaaag atggcatggc gccagccgtt ggcttctagc
      361 cagcgtttaa gaccgtagtc ttgataatcg gcataagcca gccagatacg tgtcggcgat
      421 gtttgcctga taatataatt cattttcaaa tcaagagcct cccccgatac cggccaaatc
      481 cgagattgag catcattctc taaaagccct aaagtatttc gactggcata aatatcattt
      541 tgccaatcaa ataattgcgg cggatagaaa ccataagcag aaatatgggg gtgtaaataa
      601 taattgagat tgctgcggtc ataggaaaaa ttatcaatca caatatcgcc cggcaagaat
      661 ccatcattta tctgctcagc aatcagtttt tgtgccctat cggcgtcaaa gctattctga
      721 ctcaataaaa ttttcgcatc aaaagtgata aagctgacga taaaaatact aattaaagcg
      781 gcggccgctt tagttttcag cctggaagcc aaaccggcta gagctaaagc cagcaaaatt
      841 gacagccaaa aaacgtgctt ttcaaaaata ctaacatagt tttctgaggc tggagaaaaa
      901 agaaagatga ctaaggatat aatcaccaag aaagatatgt gtaaaaaggg gaaaataatc
      961 tctttctctt tttttaataa tagccagagt aaagcggcgg cagccgcaag cttggaaact
     1021 aaaatcacta aaatttccaa aggcaagact tgtttgtctt ttaccgtcca aattaattgg
     1081 ttaaaagaag cttcaaaaaa atatagagca cgatgagaaa aaatagtgcg cggtaaattc
     1141 attaactcat aattacccaa taaaacttta tacaacatcg ccggtaacca ccaatagaat
     1201 cctaaaaata tagaggctaa aactaaaact aaacgaaata aatcttcacg acgcttatca
     1261 ataattatct taaacaacca ccacgccaac agaggaaaca aaattaacaa gaaagaatag
     1321 tgtagataaa gaccaaaaat ggcaaatata gaaaaataaa ctaactgact ccaatgtttt
     1381 ttgtctaaat aggtattaag cttatacaaa caaagaagtc ctaaaaaaga aaagattata
     1441 tagggacgag cctcggtgct aaatattatt ggcatcggca tcacagccac cagtaaggcc
     1501 gccagcaaac cggcgcgagc gtttttccac aactgttttc ctaaaaaata tgaaagcgcc
     1561 accgtagcta aaccaaaaat taaagacaaa cttctaaccg ccatctcgga aaaaccaaac
     1621 aattttgtcc aaccaataag tattaaataa tatagaggag ggtgaacttc caccgcccgc
     1681 aaatattgaa ataaaccgct aatattatct tgaaaatgtc taacaatctg taaacttaaa
     1741 atttcatcgc cccaaaaagg ctggctcaaa ttgggtaagc gtaaacagac ggccaacact
     1801 aaaattagcg ctaaaaaaaa ataatgcttg tgtaataatt tagaaatcct catatcttga
     1861 tatattttat cattaaaatt atagctagta taggcaataa aaagaaaaat taaatatcta
     1921 atcactataa aaaataagga aaaagaataa agaagtcttt accttactta attgaattac
     1981 tggtcgttat tgctattatc gcccttctta catcgctctc agtaattgca accaaccatt
     2041 ctcgaatgtt agcacgcgac gccaaacgaa aagccgattt aaaaaatagt tattgcttga
     2101 gaatgggggg taactatagc ggacaaataa tatgcgccac cgtaaaactc aaaaaacttt
     2161 aacataaaaa acctatatat tatttattgt cataaaatat ttgacattta tttaacaaaa
     2221 gatatataat tataactata taaaaaaaga attatatgga tattactatt ttaaaaaaac
     2281 ttggtctgtc agacaaagaa atgacaatat atctaaagct tttagaaggc ggccccattt
     2341 cggtcaggcg tctggcggaa aaggctaaac ttaatcgcgg tattgtctat gaagttctaa
     2401 agaccctaca agaaaaaggt ctagctagct attacaatga tactaccaag cagaaattcg
     2461 tagccgaaaa tccagaaaaa ctgcgacgtt tagctgatat tgaagtagac agagtaaaaa
     2521 acctacaaaa taatatcaac tctctaattc ttgaattaaa atccatccgt ggtcaagccg
     2581 ataatcaacc ggttaccaaa ttatatgaag aagacgctgg cctccggact atcctcaatg
     2641 atttattaga tacattaaaa gacaaagaag aaaaagaata ctatgtctat tccgctaaag
     2701 actccagtag cgatattggt cgcgctttcc caggttttaa taaagaaaga aaaaaaagaa
     2761 acatatacgt caaggctatt tctctggctg atggcggagg cacccatggt cttgatgaac
     2821 gccgttggct gggaacaaac gactgctcgg ctacttttat catcatttat gccggaaaat
     2881 gcgcttttat ctcccgtgat aagcaaggat caccggtggg cgtactgatt gaaaatcaaa
     2941 tgatttataa tacgcaaaaa acgattttcc tcaatctgtg gaaacgctta gcataatctt
     3001 atgtgtggca tcgcttctta tgtcggtcat aaaaacgctt accccctaat tttggaaatg
     3061 ctaaaacgtt tggaatatcg cggttatgac agtgctggta ttgctctata ctcccacgga
     3121 aaaatgaaaa tatataaaga acaaggagca attaccgctt tagctgaact actatcaaaa
     3181 caaaaacctt caggcagtct tggcatcggc catacgcgct gggcgactca tggatctgcc
     3241 accaaactta atgcccatcc tcatcattct caaaacaata cattaagtat tgtccataat
     3301 ggcaccatct taaactacta taaattaaaa gaagagttag aaaaggctaa ctataaattt
     3361 tatggagaaa cagatacaga aattttagtg aattatcttg attataggca acaacaaagc
     3421 aaatcttcat tattagaagt aattactgat gtcgctccta atttagaagg gcggtctgtt
     3481 tttgtaatcc ataatagcca gagcccaaat aatctttatg cctgcaatct tggcggcgaa
     3541 ctatttattg gccgcagtca agaaggagaa tattttattt gttctgataa aaacgcctta
     3601 gctggctatg ctgatgaata ttttgctcta gaaaataggc aaattgccat tgttcaaaaa
     3661 gaaaaaaatt gtcgcctctt aaatataatc tccgcagaag aactgccttg tccctgggaa
     3721 caactaaaaa tcaatttcga aactttagaa aaaggcagct atccccattt tatgcttaaa
     3781 gaaattttcg aacaagaaaa ggtagtagcg aaaacaattg acagcctgat agctgacaac
     3841 ggccaatcat taaatttaat gtttaatcat tggcggcaga aaataaaaaa aataaacaaa
     3901 ctagtaataa ttgcctgcgg tacttcttgg cacgcgggct tggtagccaa atattttttt
     3961 gctgaattag caaaacttcc cgtctattgc gaatatgcct ccgaatttct ttcagagagc
     4021 gtcggggcgg aagatatcgt catcgccatt tctcaatcag gcagtaccgc cgatacttta
     4081 caggcggttc aagcggcgaa agaacaaggc gccagcgtca ttagtatctg taatgtagac
     4141 ggctccaccc taaccagaaa tgccgatgta tccctattga cgaaagccgg accggaaatc
     4201 ggggttgcct ccaccaaggc ttttaccacc caattagtta ttctctatat tctatccact
     4261 aaattcggcc gctggcgcaa ccgattaaac gaagccgaag aaaaaaaatt atggcgggaa
     4321 ttaaaaacaa taccgaagaa aataaaagaa attcttctct ctagtccaga aatagaaaga
     4381 ctagccaaaa aatataaaga ttatcctaat tttctctttc tcggacgcgg acaaaacttt
     4441 cccattgcct tagagggggc tttaaaatta aaagaagtct catacatcca cgctgaaggt
     4501 ttatcggcgg gagaaatgaa acatggctcg attgctttaa ttgacgacca gatgccctct
     4561 ttctttattg ctgtcaaaga taaacaatac gacagagtga ttaacaactt gctggaaatt
     4621 ggcgggcgta aaggacgtat aattgccgtg acaaccgaaa aagaccaggt tattatcaaa
     4681 cgcacggatg acattatctc tatcccgcca tgcccacccc ttctctatcc ttttctcacc
     4741 gttattccta ctcagctctt tgcctattat gttgccagag aacgcggcac caatattgat
     4801 aaaccaagaa atctagcaaa agcagtgacg gtcgagtaaa aaataaaaga ccttagaata
     4861 gaggtctttt ttcataataa atatatattt agtgttgctg tttctggcga tatagttcct
     4921 tggattttc
//
LOCUS       DAOQSA010000034         4694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_19244_length_4694_cov_8.398577, whole genome shotgun sequence.
ACCESSION   DAOQSA010000034 DAOQSA010000000
VERSION     DAOQSA010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19244_length_4694_cov_8.398577"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..125)
                     /locus_tag="PKK65_03585"
     tRNA            complement(52..125)
                     /locus_tag="PKK65_03585"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(91..93),aa:Trp,seq:cca)
     gene            complement(346..486)
                     /locus_tag="PKK65_03590"
     CDS             complement(346..486)
                     /locus_tag="PKK65_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin FeS4 iron-binding
                     domain-containing protein"
                     /protein_id="HNU81779.1"
                     /translation="MSDRVNKTYFCRTCGNEVKFVKDGGGQLICCGHPMEIRKPGFDG
                     EE"
     gene            complement(497..757)
                     /locus_tag="PKK65_03595"
     CDS             complement(497..757)
                     /locus_tag="PKK65_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81780.1"
                     /translation="MSFQERLQNEPGFIDNVRYAVEIDKMSPDELLDLPIEAIQEFSI
                     EDLNAAMEKIMHGFHHLTLDKNNRSGFDVRWRKFLDTALTKI"
     gene            complement(766..1296)
                     /locus_tag="PKK65_03600"
     CDS             complement(766..1296)
                     /locus_tag="PKK65_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004853515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNU81781.1"
                     /translation="MLKEKTRKNLETAFAGESMARNKYDYFASVARKAGYVQIANIFE
                     ESALNEKEHAKLWAKQLELINEDTLKNLAEAVKGETYEYSDMYQQMAADATEEGHHEL
                     AALFRNVAKAEKAHAERYQKLYDNLETGEVFKKKEVKRWKCNNCGYIHEGTEAPEKCP
                     SCQHPQAHFEVFVETY"
     gene            complement(1317..2495)
                     /locus_tag="PKK65_03605"
     CDS             complement(1317..2495)
                     /locus_tag="PKK65_03605"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNU81782.1"
                     /translation="MTKSLTKPFLLILLGLVLVGCYFIFKPFLTEILVAAILVSIFYT
                     PYLKLIKFLKGRQNLAALLMCFFLVILIIIPTVRLVIYAGQQSATAYAQAINFFNSHS
                     VNGVIQTSALPDKLLGTINFSQYYNNDTFKNFFLDVFKQLSNWLLSGATMLVKGTTNF
                     IISLFIIIITMFFFFVDGKKMLERFMYLSPLPNVYDREIFQKFRAVSYTTILSTFVTA
                     AAQGLVGAIGFAIVGFPAFLAGMLVALLSLLPYIGSMIFYVPMGVYYLLVGKIWQGIF
                     ILLWGMIVISNIDNIIRAYMIKGKAQVNPIFVVFSILGGIALFGFWGVVIGPLIISIA
                     VTIFHIYELEFCNSLDDGCNAENKEIKIAEKKTKEEREEEAAEEKKRHKETLKKLGRR
                     "
     gene            complement(2580..2720)
                     /gene="rpmG"
                     /locus_tag="PKK65_03610"
     CDS             complement(2580..2720)
                     /gene="rpmG"
                     /locus_tag="PKK65_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNU81783.1"
                     /translation="MIKLECTECKTVNYFSRKNKKTLKERLEMMKYCDHCKKHTLHKE
                     TK"
     gene            complement(2901..3782)
                     /gene="mutM"
                     /locus_tag="PKK65_03615"
     CDS             complement(2901..3782)
                     /gene="mutM"
                     /locus_tag="PKK65_03615"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HNU81784.1"
                     /translation="MPELPEVETIRRDLEKYVIGASFQNVKFIDFKNVAPGAAFLAKF
                     LPGKKVTAVRRRGKLLILDFKDTDRYLLLHLKMTGQLIFIAGDKKIAGGHSLSNNSLA
                     AAVGGDLPNKFTRAFFDFDNGGRLFFNDLRKFGYIKLVDKKSLEKILADNYGPEPLDK
                     ALDYTYLQAIFKHREAPVKAVILNQKLIAGLGNIYADESLFMAGIRPNRSAKSLKKTE
                     IEKLREAMGNIIKKAIKARGTTFRNFVDGQGQKGNFMAQLKVYQRHGQACPLCGRPLQ
                     KIKVAGRGTHYCQYCQK"
     gene            complement(3920..4594)
                     /locus_tag="PKK65_03620"
     CDS             complement(3920..4594)
                     /locus_tag="PKK65_03620"
                     /inference="COORDINATES: protein motif:HMM:NF025698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HNU81785.1"
                     /translation="MHKIFQFGEKMEAYQVPVLNEREARAAAGILFLFAMISLSNVLL
                     AGNFTFIKIFIIAFTVEFLIRVFVNPKYAPVMILGRLAVSGQKPEYTGAPQKRFAWSL
                     GLGLALIMVASLILNIIPFTFSCLICFICVILLFLESAFGICVGCKLYNLLTGNKAQL
                     CPGGVCEARKKEDIQKVNYWQILVLLVFGLIMFAVSRLFIDNQTQATLGGCPLGSQEE
                     SGSCPF"
BASE COUNT         1565 a         1027 c          776 g         1326 t
ORIGIN      
        1 attggcttag attatacaaa aaacagagct ctaaatgaac tccgtctctc ttggcacggg
       61 atgagggaat cgaacccccg gccgctcttt tggagagagc aatgttacca ctacaccaat
      121 cccgcaattt taactaaatt tacgtaatct tttaataata aaaagctcct ttaatattct
      181 actatttcta tagataatta acaagaccat ttataacccg gtttcttatt tattccagta
      241 tgcggtttat tatacacaga aaatcaattt ttacaaatat cctttcactg gaaaatcata
      301 aaaccgccca agggacggtt ctattaattc tctataccaa aaaccctatt cttctccatc
      361 aaaacctggc tttctgattt ccatcggatg accgcagcag atcagttgac cgccgccatc
      421 tttgacaaac ttaacttcgt taccgcaagt ccggcagaaa tatgttttat tgactctatc
      481 gctcatataa aataaatcat attttagtca acgcagtatc taaaaattta cgccagcgaa
      541 catcaaaacc gctgcgatta ttcttatcca aagttaaatg atgaaaacca tgcataattt
      601 tttccatagc cgcatttaaa tcctcgatag aaaattcttg aatagcttct atcggtaaat
      661 ctagcaactc atctggactc attttatcaa tctctaccgc ataacgcaca ttatcgataa
      721 aaccgggctc attttgtaat ctctcctgaa aagacataaa tttaattaat aagtttctac
      781 aaagacttcg aagtgcgcct gcggatgctg gcaagaagga catttttccg gcgcttccgt
      841 gccttcgtgg atatagccac agttattaca tttccatctc ttcacttcct ttttcttaaa
      901 cacttctccc gtctccaaat tatcatacaa tttctgatag cgttcagcgt gggccttctc
      961 ggctttagcg acattgcgga ataaagccgc tagttcatga tgtccttctt cggtggcgtc
     1021 ggcggccatc tgctggtaca tgtctgaata ttcataagtc tcccccttca ctgcctcggc
     1081 taaattcttc aaagtatctt cattaatcaa ttctaattgc ttagcccaga gcttggcatg
     1141 ttctttttcg tttaaagcac tttcttcaaa aatattggca atctggacat aaccggcttt
     1201 acgggcgaca gaggcgaaat aatcatattt attacgcgcc attgattcgc cagcaaaagc
     1261 agtttctaaa ttctttctcg ttttttcttt taacatatta ttaatattaa ttataattat
     1321 ctccgtccta atttttttaa agtttccttg tgcctcttct tttcctcggc cgcttcctct
     1381 tcccgttctt ccttagtttt cttctcggct atctttatct ctttattttc tgcattacaa
     1441 ccgtcgtcta aagaattaca aaattccaat tcatagatat ggaaaatggt aacggcaata
     1501 gagataatca gcggaccgat aactacaccc cagaaaccaa aaagagcaat gcctcccaga
     1561 atagaaaaaa ccacgaaaat cggattaacc tgcgccttgc ctttaatcat ataggcgcga
     1621 ataatattat caatattgct aataacaatc attccccata ataaaatgaa gatgccctgc
     1681 cagattttgc caaccaaaag ataatataca cccatcggca catagaaaat catggagcca
     1741 atataaggga gtagtgaaag aagagcgact aacatgccgg ccaaaaaggc aggaaaaccg
     1801 acaatcgcga aaccaatcgc ccccactaaa ccttgagcgg cggcagtgac aaaagtggaa
     1861 aggatggtag tgtagctgac ggcgcggaat ttctggaaaa tttcccggtc atagacattg
     1921 ggcagagggg aaagatacat aaaacgctcc agcatctttt tgccatcaac aaagaaaaag
     1981 aacatggtga tgataataat gaacaaagaa ataatgaaat tagtcgtgcc ttttaccagc
     2041 attgtcgctc ccgacaagag ccaattactc aactgcttaa aaacatcgag aaaaaaattc
     2101 ttgaaagtgt cattattata atattgggaa aaattaatag tgcccaaaag cttatccggc
     2161 aaagcgctgg tctgaataac accgttaacg ctatggctat taaaaaaatt tatcgcctgg
     2221 gcataggctg tcgccgattg ctgaccggca taaattacca aacgaacagt tggaataata
     2281 atcaggataa ccaaaaaaaa acacattaaa agggcagcca gattctgacg cccttttaag
     2341 aatttgatca atttaagata gggggtataa aaaatggaaa caagaatagc agctaccaaa
     2401 atttccgtta aaaacggctt aaagatgaaa tagcagccca ccagcaccaa tcccaaaagg
     2461 atcagtaaga agggcttagt taggctttta gtcataattg catgggggcg gccgcggaaa
     2521 cggccggaat aaaaaccaaa tttataaaaa ccgagtcgcc aaagacgacc ggcagaaatt
     2581 tattttgttt ccttgtgcaa ggtgtgcttc ttgcagtgat cgcaatattt catcatttcc
     2641 aatctttcct tcaaggtctt cttgttcttg cgggaaaaat agttgacggt cttgcactcg
     2701 gtgcattcaa gcttgatcat gttgtcctgg gacatagtga aaggagaaaa cgagcgaaac
     2761 gcgcttctta aaaaaccttg aacacgcggg ttagccgttt atgtttagaa aatattaatt
     2821 actctaatac aataaataaa aataaggtaa aagtcaaccg tatggatact tatcttagtt
     2881 ataaatagaa taaatttacc ctatttttga caatattggc aataatgagt tcctcgaccc
     2941 gccactttta tcttttgcag aggccgccca cagagcggac aagcctggcc atggcgctga
     3001 tataccttta attgtgccat aaaattgcct ttttgtcctt ggccatcaac aaaattacgg
     3061 aaagtcgtgc ccctggcctt aattgccttc ttaataatat tgcccatggc ttctcgtagt
     3121 ttttcaattt ctgttttctt taagctctta gcggaacgat taggtctgat gccggccata
     3181 aacaaagatt cgtcagcata aatattgcca agtccagcaa ttaatttctg attgagaata
     3241 acggctttaa ccggtgcctc ccggtgttta aaaattgcct gcaaataagt ataatctaaa
     3301 gctttgtcca gcggttccgg gccataatta tccgctaaaa tcttttctaa agatttttta
     3361 tcaactagtt tgatataacc gaatttgcgc aaatcattaa aaaataaacg accgccatta
     3421 tcaaaatcaa agaaagcgcg agtaaatttg ttaggcaaat ccccgccaac cgccgccgcc
     3481 aaagaattat tagataaact atgtccaccc gctatctttt tgtcgccagc aataaatatc
     3541 agctgtccag tcatttttaa atgcaaaagc agatatctgt ctgtgtcttt aaaatctaaa
     3601 atcaaaagct taccccgtct ccgcacggct gtcactttct tgccgggcaa aaacttagcc
     3661 aaaaaagccg ctccgggagc gacgtttttg aaatctataa attttacatt ttggaaacta
     3721 gcgccaatta catatttttc caaatctcgg cgtatagttt ctacttcagg taattccggc
     3781 atactacaaa ataaaaacga gaaacaaaat caccgccaaa gaaaataact caacggcaaa
     3841 aatcttagtc aaactccaac cgattttatc aggatcaatt aagggcatat aattttatta
     3901 taacaaagaa aagcctaaat taaaacggac acgaacctga ttcttcttgg gaaccgagcg
     3961 gacatccccc caaagtagcc tgtgtctgat tatctataaa caatctagaa acggcaaaca
     4021 taatcaaacc aaaaaccaat aaaactaaaa tttgccaata attaactttc tgaatatctt
     4081 ccttcttcct ggcttcacag actccgcccg gacataattg tgccttgttg ccggtcaata
     4141 aattatacag cttgcaaccg acgcaaatac cgaaagcact ctccaaaaaa agtaaaataa
     4201 cacaaataaa acaaataagg cagctgaaag taaaaggaat aatatttaat attaagctcg
     4261 ccaccataat taaagctaaa cctaatccta aactccaggc aaaacgcttc tgcggcgcgc
     4321 cggtgtattc cggtttctgt cccgataccg ccaatctccc taaaatcatc actggcgcat
     4381 atttgggatt aacgaaaacc ctaattaaaa attcaactgt gaaagcgata ataaatattt
     4441 tgataaaagt aaaatttccg gctaataaaa cattagataa ggaaatcata gcaaataaaa
     4501 acaaaatacc ggcagcagca cgcgcttccc tttcgtttaa aaccggcact tgataggctt
     4561 ccattttttc gccaaattga aatatcttat gcataaaaat aaattaatta ataattaata
     4621 agtaaaatta taccctatag ctaattctaa ctcaaacaaa aattctccca ataaggaaga
     4681 attataatgg tggg
//
LOCUS       DAOQSA010000035         3520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_29666_length_3520_cov_7.454257, whole genome shotgun sequence.
ACCESSION   DAOQSA010000035 DAOQSA010000000
VERSION     DAOQSA010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29666_length_3520_cov_7.454257"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PKK65_03625"
     CDS             <1..204
                     /locus_tag="PKK65_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81786.1"
                     /translation="FENIFVFNGLPAHEIIFLYRVKFKSKEAYRRLLFPILDSDHGHQ
                     AIWINPKKVGVKKIYPQEILDYI"
     gene            209..2329
                     /locus_tag="PKK65_03630"
     CDS             209..2329
                     /locus_tag="PKK65_03630"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNU81787.1"
                     /translation="MHDLKSLFSPRSIAVVGASTRPESVGRALFENLRLSGFKGKIFP
                     VNPKVKRLGSLRCYSSLSEIQEKIDLALIAVPAILVPDILREAGRIKIKAAVIISAGF
                     KEAGRNDLEQEIVRIAKHYRLTLLGPNCLGFIRPRNCLNATFASVSSTPGSLAFLSQS
                     GALGTAFLDKAQSLSLGVSLFASVGNKAILDEADIINYLQTDKSTKVLGLYVEQFSRP
                     EILGRAIRRFNRGAGARPVVVLKAGATAAGAQASVSHTGAMAGNDEIYQAFFRDNSII
                     RADSMSDFFDYLQIFTSNKIVSARRLAIITNAGGPGVLAADAASRRGLILSQLGRATL
                     ASLKKNLPAAASMANPIDILGDASPKRYLDTLKMILTDDAVESILILVTPQSMTKIEE
                     LAEILLKHRRSLSKPLAICLMGGASMSSASLSLRRAGLAVFDYPEQAVAALAVLADFY
                     DKEKKKVSNVFQIKMPSISWPEKRAFERVTKIFAHLRSQGIKEIPEFQALEILRLYDL
                     PVPKFFILRSQADAARARKIFPEPLALKIASYDILHKDEVGGVSLDVKPENLVGAYKD
                     ILARVKERKPGAAIEGVLVMPMVSSSLAELIVGSVSDSSFGHSLMIGWGGIYTETIKD
                     VSFTLLPLTLEKIRRMLSTLRVGKILLGSRGQKPADFQSLVRLLFTLERLLFDFPEIK
                     EIDLNPVAISAEGLQILDARLILS"
     gene            2342..3121
                     /locus_tag="PKK65_03635"
     CDS             2342..3121
                     /locus_tag="PKK65_03635"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNU81788.1"
                     /translation="MYMNLLWFLPTFIILYMTFWFLVSLLVERRDIIDIAWGAGFVFL
                     AWTTLIFRDLDLDTRSILLLSAISLWGLRLSFHTVRRNLRQEEDWRYHRWSQGQARLH
                     PFCVFFQVFLLQGALMMIIALPVVYSLCFINLPLFELNYLGIFLIIFGLIFETVADQQ
                     RYLFLKKERNKDQLISTGLWRYSRHPNYFGEIIFWWGIYFLVLGAPKSWMMIISPLTI
                     TYIMLFVSGLPMEERYRGRPDFEEYKRRTSALIPWFVKKNR"
     gene            3335..>3520
                     /locus_tag="PKK65_03640"
     CDS             3335..>3520
                     /locus_tag="PKK65_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81789.1"
                     /translation="MKHILYNQVKEHRLGGWLVFTAMRLEMMADRFMFKPLGLTTASF
                     RILMLLDKLGPQTPSEII"
BASE COUNT          886 a          644 c          788 g         1202 t
ORIGIN      
        1 tttgaaaata tctttgtttt taatggattg ccagcacatg aaattatatt tttgtatcgg
       61 gtaaaattta aatcaaaaga ggcttaccgt cgccttttat ttcctattct tgattctgac
      121 cacggtcatc aagcgatatg gataaatcct aaaaaggtag gcgttaaaaa aatatatcct
      181 caagaaattt tagattatat ttagatttat gcatgattta aaatctttat tttctccacg
      241 ttccattgct gttgtcggtg cttcaacccg ccctgaaagc gttggtcggg cactttttga
      301 gaatctccgt ttatcgggtt tcaaaggaaa aatttttcct gttaatccaa aagtaaaaag
      361 actagggtcg ctacgttgct attcttcttt gtcggaaatc caggaaaaaa ttgatttagc
      421 gctgattgcc gttccggcaa ttttagtgcc agatattttg cgggaggcgg gaaggataaa
      481 aattaaggct gccgtaataa tttccgccgg ttttaaagaa gcaggacgta atgatttaga
      541 gcaagaaatt gtccgtatcg ctaagcatta tcgcctcact ttacttggac ctaattgtct
      601 tggctttata agacctagaa attgtcttaa tgctactttt gcttcggttt cttccactcc
      661 cggcagttta gcctttttat ctcagagtgg cgctctaggc acggcttttt tggataaggc
      721 ccaatctttg tctttaggtg tttctttgtt tgccagtgtt ggtaacaaag ctattttgga
      781 tgaagccgac attattaatt atttacaaac tgacaaatca acaaaagtcc tcggacttta
      841 tgtcgaacag ttttcgcgtc cagagattct gggtagagct attcgtcgtt ttaatcgtgg
      901 cgccggcgct aggccagtgg tggtattaaa ggcgggggct actgccgccg gtgctcaagc
      961 cagcgtttct cataccggtg caatggcagg aaatgacgaa atttatcagg ctttttttag
     1021 agacaatagt attattaggg cagatagcat gtcagatttt tttgactatt tgcaaatatt
     1081 tactagtaat aaaattgttt cagcgcgccg tttggccatt atcactaacg ccggcggacc
     1141 cggcgtgcta gcggctgatg ccgcttctag acgcggttta attctcagtc aacttggacg
     1201 ggccactctt gcttctttga aaaagaattt gccggcggca gcctctatgg ccaatcctat
     1261 tgatattctt ggtgatgcca gccccaagcg ctatttagat actctaaaga tgatattgac
     1321 tgatgacgcg gtcgagtcta tcttgatttt ggtaacgccg cagtcgatga caaaaattga
     1381 agaactggca gagattttac ttaaacatcg gcgctcgctt tccaaaccac tagccatttg
     1441 tctgatgggc ggggcatcta tgtcttctgc ttccctatct ttacgccgcg ccggtttggc
     1501 cgtttttgat tatcctgagc aagcggttgc cgcgctggcg gttttagcgg atttttatga
     1561 taaagaaaaa aagaaagtat caaatgtttt tcagattaag atgccgtcta tttcctggcc
     1621 ggaaaagagg gcatttgaac gggtgactaa aatatttgca catcttcgct cgcaagggat
     1681 caaggaaata cctgaatttc aagccttaga aattttacgt ctttatgatt tacccgtgcc
     1741 taaattcttt attttaagaa gtcaggctga tgccgccaga gcgagaaaaa tatttcctga
     1801 gcctttggct ctaaaaatag catcttatga tattttgcat aaggacgagg ttggcggcgt
     1861 gagtcttgat gttaaaccag agaacttggt tggggcttat aaggatatcc tggcaagagt
     1921 taaggagaga aagcctggtg ctgctattga gggtgtatta gtaatgccca tggtatcctc
     1981 ctctttggca gagcttattg tcggtagcgt aagtgattcc tctttcggcc attctttaat
     2041 gattggctgg ggcggtattt acacagaaac gattaaggat gtatctttta ctcttttacc
     2101 tctgactttg gaaaaaatta ggcggatgct gtcaacactg agggtaggga aaattttgct
     2161 tggtagccgg ggtcaaaagc ctgctgattt tcaatcattg gttagattgt tatttactct
     2221 tgagcggtta ttatttgatt ttccggaaat caaggaaatt gatttgaatc cggtcgccat
     2281 ctcagcagag ggcctacaaa tacttgatgc gcgtttaatc ttatcttaaa tatttaaaaa
     2341 tatgtacatg aacttgcttt ggtttttgcc cactttcatt atcctgtata tgactttttg
     2401 gtttttagtc tcgttattag ttgagcgtcg cgatattatt gatattgcct ggggagcagg
     2461 ttttgttttt ttagcttgga caactcttat ttttcgcgat ttagatttag acacgcgctc
     2521 cattttatta ttatccgcta ttagtctttg gggcttgcgc ttgtctttcc ataccgttcg
     2581 ccgcaatctt cgtcaagaag aagattggcg ctatcatcgc tggagtcagg ggcaggcacg
     2641 tctgcatccg ttttgtgtgt tttttcaagt atttttatta cagggagcct taatgatgat
     2701 tatcgcctta ccggtggttt acagtctgtg tttcattaat ctgcctttgt ttgagcttaa
     2761 ttatctcgga atttttttaa ttatttttgg tcttattttt gaaaccgtgg cagatcagca
     2821 acgttatctt tttttaaaaa aggagcgcaa taaagaccaa ttaattagta ccggattatg
     2881 gcgctacagc cgccatccta attattttgg ggaaattatt ttttggtggg gtatatattt
     2941 tttagtttta ggagcaccaa agtcatggat gatgattatt tctcctctaa ctattactta
     3001 tattatgctt tttgtttccg gtttaccgat ggaagagcgt taccgggggc gtccggattt
     3061 tgaagagtat aagcgccgga ccagcgcctt aattccttgg tttgtcaaaa agaaccgtta
     3121 gctggctctt gataaggtca ggaaaccccg gataaagcca tccctgaaag ggtggctttt
     3181 tgatacgggg ataagatcat taaaagtggt cttgactgtt ttatagttta ttgataaact
     3241 atatttttga taaactatat tttgatgttt gggtttggcg ccgctcttga aattataggc
     3301 gcataatatt tgtttgaatt ttttagataa ccttatgaag catatacttt acaatcaagt
     3361 taaggagcat cgtttagggg gttggctggt ctttaccgct atgcgtttag aaatgatggc
     3421 agatcgtttt atgtttaagc ctttgggctt gactacggct agttttagaa tattaatgtt
     3481 gcttgataag cttggaccgc agacgccctc ggaaatcatt
//
LOCUS       DAOQSA010000036         2654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_45838_length_2654_cov_7.981531, whole genome shotgun sequence.
ACCESSION   DAOQSA010000036 DAOQSA010000000
VERSION     DAOQSA010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45838_length_2654_cov_7.981531"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(252..1241)
                     /gene="trpS"
                     /locus_tag="PKK65_03645"
     CDS             complement(252..1241)
                     /gene="trpS"
                     /locus_tag="PKK65_03645"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNU81790.1"
                     /translation="MNQSKTIFSGVKPSGDLTIGNYIGAISQWPEMQDKYRCIFSIVD
                     YHAITVKYQPEELRKRILEITKIYLASGIDPKKSIIFQQSAVPEHTELAWILGCSGSY
                     IADLKKMTQFKDKAGEKQNSVFVGLFTYPVLMAADILLYDTQAVPVGDDQKQHVELTR
                     DIAKRFNNKYGPSFVVPEAIVRKEGARIMSLTEPIKKMSKSASNSNSYISLLDDIEVA
                     KKKIMKAVTDSGTNIDYDSANRPALANLLTIYAQLGNRQIPRLVKDYQGKGYGEFKKD
                     LAEIVAKFLTDFQNKFNNISNKEAQAVLESGRQQASALAQMKMEKIRKVIGVR"
     gene            complement(1655..1867)
                     /locus_tag="PKK65_03650"
     CDS             complement(1655..1867)
                     /locus_tag="PKK65_03650"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU81791.1"
                     /translation="MELVDDPNVKPYDSQKETIWDGVGILDYTILPHYKSDHPESGKV
                     DEAIEYMTKNKIPFKTLRDGEVIIIE"
     gene            complement(1873..2277)
                     /locus_tag="PKK65_03655"
     CDS             complement(1873..2277)
                     /locus_tag="PKK65_03655"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HNU81792.1"
                     /translation="MKFYLSSYKVGNETDKLKALIPADNKKTAYISNALDFSNDAERR
                     KQGEQSDIEQLKPFGLDVELLDLRDYFGKKTELEKKIAEFGVIWVRGGNCFVLRQAMK
                     LSGLDDILKDLLKKMIFFTEDIAPEFAFWLQP"
     gene            2425..2498
                     /locus_tag="PKK65_03660"
     tRNA            2425..2498
                     /locus_tag="PKK65_03660"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2457..2459,aa:Pro,seq:ggg)
     gene            2568..2643
                     /locus_tag="PKK65_03665"
     tRNA            2568..2643
                     /locus_tag="PKK65_03665"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2601..2603,aa:Ala,seq:cgc)
BASE COUNT          760 a          563 c          432 g          899 t
ORIGIN      
        1 taacaaggca tccgtagcgg aagctgtgga tggatcacct cctttctagg gagtaattgt
       61 ctttcatcat tgatgaaaca agacaatagt cgatattgta cctttggtac aataaatatt
      121 atcgcaagat aatacaaaaa tattattatc ttgaattgat gccgattaga ctcaaaattt
      181 tattaccagc taactttaca aaaaaccgct ttattggcgg ttttttgttt gaatattgaa
      241 taattacttt tttatctcac accaattact tttctaatct tttccatctt catttgggct
      301 aaggctgacg cttgttgacg gccgctctct aaaactgctt gcgcttcctt attgctgata
      361 ttgttaaatt tattttggaa atcagttaaa aacttagcga ctatttccgc taaatccttc
      421 ttaaactccc cataaccctt tccttgataa tcttttacta aacgcggaat ttgacgatta
      481 cctaattgag catagatggt gagaaggttg gctaaagccg gacgattagc ggaatcataa
      541 tcaatgttgg ttccggaatc ggtcaccgct ttcattattt tctttttagc gacttcaata
      601 tcatcaagta aagagatata actgttagaa ttgctggcgc ttttggacat tttcttaatc
      661 ggttctgtta gactcataat gcgcgcgcct tctttacgca caatcgcctc cggcaccaca
      721 aaagaaggac catatttatt gttaaatctc ttggcaatat cacgcgtcag ttcgacgtgc
      781 tgtttttgat cgtcgcccac tggcactgct tgcgtatcat ataataatat atcggccgcc
      841 atcaaaacag gataagtgaa gagaccaaca aaaacggaat tttgtttttc tccggcctta
      901 tctttaaatt gtgtcatttt tttcaaatcg gcgatataag aaccagaaca acccaatatc
      961 caggctagtt ccgtatgctc aggtacggct gattgctgaa aaatgattga ttttttcggg
     1021 tcgattccag aagcaagata aatcttggta atttctaaaa ttctctttct taattcctct
     1081 ggctgatatt tgacggtaat agcatgatag tcgacaatgg aaaaaataca gcgatattta
     1141 tcctgcatct ccggccactg actgatggcg ccaatataat taccgatggt caaatcgccg
     1201 ctgggcttga ctccggaaaa aattgtttta gactgattca tataattata ctttatatta
     1261 atattctact ttaatccccc gccaaaagca attttccgga aattcttaaa caacaaaatc
     1321 aatatcttta taatttctca actccttcaa aatacgacag taaggtcaaa aaacctgctt
     1381 tcgggtattt tttcccaatt tttttgacga tttgataatc caaataagct tcttccttga
     1441 taactctttt ttattacggc aacgctgtct gtatcggaaa tatccggttt tgccttgccc
     1501 acactgacgc ttctccgaat ataaatactt aacaaattat cgccaagctt atcattacat
     1561 aatttgttta tttcttctaa aattttccta gttgagcctt tcccaacccg gcttcattaa
     1621 caacaaaacc atttttatct attttataca gcatttattc aataattatt acttccccat
     1681 ccctaagcgt cttaaaagga attttatttt ttgtcatata ttcaatggcc tcatctactt
     1741 tcccggattc cgggtgatca gatttataat gaggtagtat tgtataatcc aggataccga
     1801 ctccatccca aatagtttct ttctggctat cgtatggttt tacattcgga tcatccacca
     1861 attccatacc ttttagggtt ggagccaaaa cgcaaactcc ggcgctatat cctccgtaaa
     1921 gaatatcatc ttttttaata gatccttcaa aatatcatct agcccgctta gcttcattgc
     1981 ctgccttaaa acaaaacaat ttccgcccct tacccaaatc acgccgaatt cagcgatttt
     2041 tttctccaat tcggtttttt taccaaaata atctctcaaa tcaagcaatt caacatccaa
     2101 accaaacggt tttagctgtt caatgtcgga ttgctcgcct tgttttctcc tttcggcatc
     2161 gtttgaaaaa tcaagcgcat tggaaatata tgctgttttt ttattatccg caggaatcaa
     2221 cgcctttagt ttgtctgttt cattgccgac tttataagat gatagataaa atttcatatc
     2281 tgttatatta ataactatag taatatctta tatgtcccaa ccaatgagag caaggtaaga
     2341 ttatttatgt cttaatgcgt tattattaat gcgtaattgt tgacttatct tagaaatatc
     2401 cttacactat tgctatcgtg ttttcggggt gtagtataac ggtagtacgc atgcatgggg
     2461 tgcatgtagc ccgggttcaa ttcccggcac cccgaccaaa aaatacaaaa aaatcaactt
     2521 attgcccagg tggtccatat aattttacag actcatttac atcggcaggg cttgtagctc
     2581 atctggtaga gcgctgcatt cgcattgcag aggtaagggg ttcgagtccc ctcaagtcca
     2641 ccacgggctg tggt
//
LOCUS       DAOQSA010000037         2013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: bacterium isolate AMR_MDS_1178
            NODE_71231_length_2013_cov_8.534219, whole genome shotgun sequence.
ACCESSION   DAOQSA010000037 DAOQSA010000000
VERSION     DAOQSA010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852521
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      bacterium (wastewater metagenome)
  ORGANISM  bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 09:01:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 736
            CDSs (total)                      :: 697
            Genes (coding)                    :: 691
            CDSs (with protein)               :: 691
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 6 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71231_length_2013_cov_8.534219"
                     /isolate="AMR_MDS_1178"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1869227"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..681)
                     /locus_tag="PKK65_03670"
     CDS             complement(106..681)
                     /locus_tag="PKK65_03670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNU81793.1"
                     /translation="MSKYLIIYAHPNHEGHCGYLLSRIQEKLEKNKADWEIIDLYALN
                     YDPVLKDSELYSAGRREVSPENLGFQEKIKAADRLLFIYPTWWQNMPAILKGFLDRVF
                     SSRFGFVYKNGLPVGLLKGKKAAAFTTSGGPSLYTHFITGSSSLRVLLKHTLGFCGIQ
                     TKGFLLGRAQKLENNKERLDNMADKIMKYLQ"
     gene            complement(735..1073)
                     /locus_tag="PKK65_03675"
     CDS             complement(735..1073)
                     /locus_tag="PKK65_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNU81794.1"
                     /translation="MAKQDKYKCHAAIQATLEIIGGKWKPLILWHLLAKKMRFNELQK
                     SLGKISQKTLANELRSLEKDGLVHREVYRQVPPKVEYWITPDGRSLEKVLSAAANWQH
                     AHRLKFKKNQ"
     gene            1195..1875
                     /locus_tag="PKK65_03680"
     CDS             1195..1875
                     /locus_tag="PKK65_03680"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNU81795.1"
                     /translation="MKQTLLIILLGLGLVALAVYITSRVKPADKDDNKRASAKIKVEG
                     DGQSRPVALLDGSYQLDLNASWLTWHGEKVIGKSHTGTTAFQSGSLQVQDGKLSGGEF
                     IIDVASLKDNDNIEMLGNHLKSADFFDVAHYPTAKLLITKAEAGKDGHYKIDADLTIK
                     DVTAPVSFEAILSPTVNGLSATSQFEIDRTTWGLKYGSGKFFQNLGDNMINDEIVFTI
                     DLQATRAN"
BASE COUNT          539 a          384 c          436 g          654 t
ORIGIN      
        1 aatgaagtta gggtggatgt tactttgacc tacgaagtta aataatgtct cttttatcat
       61 taaagccgct tctttaggga agcggctttt tgtttatctg tctttttatt gcaaatattt
      121 cattattttg tcggccatat tatccaatct ttctttattg ttttctagtt tttgggctct
      181 tcccagcaag aaacccttag tctggatgcc gcagaatccg agcgtatgtt ttaatagaac
      241 tcgcagggaa gagctgccgg taataaagtg ggtgtacaga gagggtccgc cgctggtggt
      301 aaaagcggcg gcctttttgc ctttaagtaa gccgaccggc agaccgtttt tatagacaaa
      361 accgaaacgg ctagaaaaga ctcggtcgag gaaaccttta aggatagcgg gcatgttttg
      421 ccaccaggta ggatagatga aaagcaggcg gtcggcggct tttatttttt cttgaaagcc
      481 aaggttttcc ggactgactt ctcgccgacc ggcgctatag agttctgaat ctttaagaac
      541 agggtcatag tttaaagcgt agaggtcaat aatttcccag tcggctttat ttttttccag
      601 tttttcctga atgcgactaa gcagatagcc gcaatgaccc tcatgattag ggtgggcata
      661 gataattaga tattttgaca taatttttaa ctagtcgttg cgctcggatt tttacccgaa
      721 agacagcggc tttattattg atttttttta aatttaaggc gatgggcgtg ttgccaatta
      781 gccgccgccg acaatacttt ttccaggctg cgtccgtcag gagtgatcca gtattccact
      841 ttcggcggca cctggcggta gacctcgcgg tgaacgaggc cgtctttttc caaagatcgt
      901 aactcgttcg ccaaagtttt ctgggaaatt ttgcccagac ttttttgaag ctcgttaaaa
      961 cgcattttct ttgccaacag gtgccacaga atcagaggct tccatttgcc gccgattatt
     1021 tccagagtcg cttggatagc cgcgtggcac ttgtatttgt cttgtttagc catatagtta
     1081 cttttattac tgtattatac attaaagcat gtacttgcgt caatatctgt ataatgttat
     1141 aatgaagcaa ctttataatt aatctcggtc ttataattta agatcaataa tcatatgaaa
     1201 caaactttat taattatact cctaggctta ggcctagttg ctctcgccgt ttatatcact
     1261 agtcgtgtga agccggcaga taaagatgat aacaaaagag caagtgcgaa aattaaagtt
     1321 gagggagacg gtcagtctcg ccccgtcgct ttattagatg gttcatatca attagacctg
     1381 aacgcttctt ggcttacttg gcatggtgaa aaagtaattg gtaaaagcca taccggtacc
     1441 acagctttcc aatctggaag tttgcaggtt caagatggta aattaagcgg cggtgaattt
     1501 attatcgatg tcgcctcttt aaaggataat gataatatcg aaatgttagg taatcacttg
     1561 aagagtgctg atttctttga tgtggctcac tatcctaccg ctaagctttt aattactaaa
     1621 gcggaagcag gcaaagatgg gcattataaa attgatgccg atttaaccat taaagatgtg
     1681 accgctccgg ttagttttga ggctatatta tcgcccactg ttaatggctt atctgccacc
     1741 agtcaatttg agattgatcg taccacctgg ggcttaaaat atggctctgg taaatttttc
     1801 cagaatttgg gagataacat gattaatgat gaaattgtct ttactatcga cctacaagcg
     1861 actagagcaa attaattatc cgctcgatag ttattttaaa aagggcggtt taaaaccgtc
     1921 ttttttattg ttaaactcta gatttttttt ctcttttctg ttacaattgc agcaattagg
     1981 gttacctttc gggttccgaa cccatataat atg
//
