LOCUS       DAOQSI010000001        48231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_344_length_48231_cov_6.207697, whole genome shotgun sequence.
ACCESSION   DAOQSI010000001 DAOQSI010000000
VERSION     DAOQSI010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 48231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48231
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_344_length_48231_cov_6.207697"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="PKL42_00005"
     CDS             complement(<1..583)
                     /locus_tag="PKL42_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNU65475.1"
                     /translation="MTLPKALPNDLIDSLLSNYKKPEDLIGENGLLKQLTKALVERAL
                     QAEMAEHLGHDKHEAVTNASGNARNGKSRKTLKGEFGELPIEVPRDREGSFEPQLIPK
                     HQTRWTGFDDKIISLYSRGMTVREIQAHLTDMYGTEISPTLISTVTDAVVDDVKQWQS
                     RPLDAVYPVVYLDCIHVKVRDAGAVRAKAVYLAI"
     gene            complement(692..1537)
                     /gene="ttcA"
                     /locus_tag="PKL42_00010"
     CDS             complement(692..1537)
                     /gene="ttcA"
                     /locus_tag="PKL42_00010"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HNU65476.1"
                     /translation="MNQYSFNKLQKRIRRDAGKAIMDFNMIENGDLVMVCLSGGADSY
                     TMLDTLVYFSKIAPVDFKVVAVNLDQKQPGFPADVLPTYLKKIGVDFKIVEQDTYSVV
                     KEKIPEGKTTCSLCSRMRRGILYKTANELGASKIALGHHRDDILETLLLNLFYGGSLK
                     AMPPRLRSDEGNNIVIRPLAYCVEKDIKQYSQMMQFPIIPCNLCGSQPNLQRQVMKEL
                     LGKWSKENPGRVESMFRAVCNVSPSHLLDRELFDFTNFKPKNKQSYETEFIVKQEAEN
                     IIFID"
     gene            1884..2666
                     /gene="ttcA"
                     /locus_tag="PKL42_00015"
     CDS             1884..2666
                     /gene="ttcA"
                     /locus_tag="PKL42_00015"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HNU65477.1"
                     /translation="MVMLISKDSHAMLMLLLALQARAPIDFKLIAMNLDQKQPGFPAD
                     ILPAYFEKLGVDYRIVEADTYSIVKEKIPEGKTTCSLCSRLRRGVIYTTAKELGANKI
                     ALGHHRDDIVETLFLNLFFGAKMKAMPPKLSTNDKQNIVIRPLAYCSEKDIASYARQM
                     DFPIIPCDLCGSQENLQRQKVKEMLHSWEREQPGRINNIFRAIGNVEPSHLADFNLYD
                     FKNLSQAKPEDEDPLFGDIANEGAALSINNTAGTRIEFVRNT"
     gene            2807..5398
                     /gene="topA"
                     /locus_tag="PKL42_00020"
     CDS             2807..5398
                     /gene="topA"
                     /locus_tag="PKL42_00020"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNU65478.1"
                     /translation="MSKLLIVESPSKAKTLKKYLGSDFEVLASYGHVRDLIPKNGAVD
                     TEHDFAMKYDIIERNSKHVDAIAKAVKSADSIYLATDPDREGEAISWHIAEILKSKNL
                     LKNKLMKRVVFHEITKTAVEHAIAEPRDISMPMVNAQQARRALDYLVGFNLSPLLWKK
                     IRRGLSAGRVQSPALRLIVERELEIEAFKSQEYWSIHLDALKHSHGFSAKLIQLNHEK
                     VEQFTVINHDQQNDIVGKLLLASAGKTTVSRVEKKQRSRSPAAPFTTSTLQQEAVRKL
                     GFTTSRAMRIAQQLYEGMDVGNGTVGLITYMRTDSFSIAAEAIAQIRDYVKKNFDTEY
                     LPKTAIAYKTKSKSAQEAHEAIRPTDINRTPASVRQYLTDEQFKLYEMIWKRALACQM
                     SPAKFDAVSVDLSVGSDANLFRASGQTLVFPGFIAVYMEGTDDEEEEAEAKLPHLETG
                     EVLTVEKIYGEQHFTEPPPRYGEASLVKILEEYGIGRPSTYASIISTLQDREYVILDK
                     KRFKPTDVGRVVNKFLTEHFTKYVDYDFTANLENELDEIAEGDREWIPVMDEFWHGFN
                     QQLLSKADVDRPGNELIDEACPKCGKPLQKQLSRYGSFIGCTGYNDTPKCDYKRSIGG
                     EAAVGADPIIIGSDDETGKEIMLMNGPYGPYLQLGAVIEGDKKKPKRVSVPKEISLAD
                     MSLETAKMVLSLPRDLGQHPETGKKIVANIGRFGPYVNHDGKFKSIPKTESVFTIDLA
                     GAVTLLAQAHSGPAPIADLGPHPSGEGRIEIYAGRYGPYVQHAKIRATLPKSVEPEAL
                     TLEEAIELLNAKALKDAPAKKTTAKKVVAKKPATKKEAVKKVATKKSVTKKAVTKKPV
                     AKKPAKE"
     gene            complement(5589..6662)
                     /gene="pbpG"
                     /locus_tag="PKL42_00025"
     CDS             complement(5589..6662)
                     /gene="pbpG"
                     /locus_tag="PKL42_00025"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HNU65479.1"
                     /translation="MRKFTLIYLAFMLALTPVASLAASKQKQKNVASNAKVSNAKYTL
                     NKNVTGKYKLTKHSPSAKLKGSKLNASRKSNMHVKLRKPVYVQPTETFEDLYDGNGPL
                     QLASAKALVINQYTGEVIYEKNTKEVTPIASVTKLMTAMVVLDAKLPMDEVLYISEDD
                     IDYLKGTRSRLSVGTALTRAELLRLALMSSENRAASALAHNYPGGIDAFLRAMNRKAT
                     SLGMRSTKFYDATGLDSNNVSTAEDLVKMVKTAYLYDEIRQESTTASQEMSLLNHDYP
                     VNFVNTNALVRAGEWMIGLSKTGYISEAGRCLVMQAEISGQSLIIILLDAAGKSTRIG
                     DANRVRKWVENNKTEVLGEHVSG"
     gene            6869..7249
                     /gene="gcvH"
                     /locus_tag="PKL42_00030"
     CDS             6869..7249
                     /gene="gcvH"
                     /locus_tag="PKL42_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNU65480.1"
                     /translation="MSIPKTLHYSKEHLWTQADVDGSWQAGITDYAQDLLGDIVFVDA
                     PAVGTKLVAGQPCGLVESVKTGSDLHALVDGEVVAINVEVINAPERINDKPYEAWIFK
                     FKPLEANVEAQLLSSEDYQKLISA"
     gene            complement(7404..8606)
                     /locus_tag="PKL42_00035"
     CDS             complement(7404..8606)
                     /locus_tag="PKL42_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65481.1"
                     /translation="MKEDDIPVLTEIAGADKSSAKSIPLSPNLIAEIIAQIRPHLEDE
                     IEKSVIPKLRKKLQEDILENLHNESANIQKANQAYMASALSQQFEQQLARLDKQFEAI
                     QLEKESALKSFVSNELANAQRASHDFLSETIYTELEQTQQTLAAHIKQTVNQELSQAV
                     TSAEQSLLANTANFVDKTKADLATELPAMLHHSAAIVKADIEQTLNELQAQGIATVQA
                     NLTQALPAMEQGLAEQLQAKLASLETLTVENATDSLQSKIAQLNEDILAERQTNLPTE
                     LADIYHQLTQQTHSELSVFLDALQVESQQALEQKLGDTFPALYQGLSNELSASLREDY
                     MTMAAEAKNEFKRGLTAELPEVEQALDNKVHEILDAEVPRIEETLTANIKAEIEKMVE
                     SVRLVFPK"
     gene            complement(8603..9043)
                     /locus_tag="PKL42_00040"
     CDS             complement(8603..9043)
                     /locus_tag="PKL42_00040"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HNU65482.1"
                     /translation="MTRVEFYFNVADKLAKSAELCEKATNKGRQLTLLTQDEQMNSAL
                     QQYLWQHSATSFLANAKATEAHSAFSAILLDCSGENLQQDDILINLQNQHPPFFSRFR
                     HLVEIVGTDESDRIAARQRFKFYKDRGYAIKATDEKTGEVKDWK"
     gene            complement(9065..10561)
                     /locus_tag="PKL42_00045"
     CDS             complement(9065..10561)
                     /locus_tag="PKL42_00045"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HNU65483.1"
                     /translation="MKFSIKNGSAAKELNDCVILGVYESQELSAAAKQVDAVAEGFLT
                     KILKRGDFDGKLESTLMLHDVANVASARVMLVGLGKAAGFTEKQYCKAVRASLKALVK
                     SGAKNAATFLAELTVKKLTAERRVALLVEATLDAHYQFDAIKRKKVKADTKNIQLSMD
                     ISVAETSEIAQANAGLVYGEAVALGVSLAKDLGNLPPNVCNPTYLAEQALGLGQQYGL
                     QVEVLEREALEKLGMGSFLGVSQGSETPPKFIIMQHLKGSVGEKPVVLVGKGITFDTG
                     GISLKPGAEMDEMKYDMCGAASVLGTFKTIAALDLKLNVIGLIPTCENMPDGRATRPG
                     DVLTSMSGLTIEVLNTDAEGRLILCDALTYAERFEPSAVVDIATLTGACVIALGHHAT
                     GLFSNQDALAHELLRAGEAALDRAWHMPMWEEYQPLLESNFADLANIGGRAAGSITAA
                     CFLSRFAEKYHWAHLDIAGTAWKSGKEKGGTGRPVPLLTAFLVNRANQ"
     gene            10685..11794
                     /gene="lptF"
                     /locus_tag="PKL42_00050"
     CDS             10685..11794
                     /gene="lptF"
                     /locus_tag="PKL42_00050"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HNU65484.1"
                     /translation="MLFKRSLIQELSASAAGAFLILTGIVIAQRAGFLIRHAAKGIIP
                     NDAITTMLGFNMIRFLPMLLSLSLFLAILLTLSRWYRDSEMVIWFSAGLSTNSLIRPI
                     LNFTIPIVIIISLLSFFVTPWAINKVEDYRVQLESRDDLSTINPGVFKESAHADRVFF
                     VESFDELGNVVKNIFVQSIQHEKLGIIVAAKGSRLVEKNGDNFLLMQNGRRYQGVRGT
                     AEYSTTEFEKYAIRVEAVEAQRDAPTTQSISSFELLKNSNNANIAELQWRLSMPIAAL
                     ILAILAIPLSAVDPRAGRSANFALAIIIYILYINLLSIIQAWIAQDKISGIVGLWPVH
                     FIFGSVAIYMVYRRMQQLTLLPTFMIKAWGKLKRT"
     gene            11804..12874
                     /gene="lptG"
                     /locus_tag="PKL42_00055"
     CDS             11804..12874
                     /gene="lptG"
                     /locus_tag="PKL42_00055"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HNU65485.1"
                     /translation="MKIIHHYLLKEIVANILLVMLALIAMFSFFDLIQELDALGKGNY
                     GLGQVLLFVLLSAPGHVYEVIPVAVLVGTMLTLGLLSRYSELIILRVSGLSIFNIAVM
                     LLKISLVFTLITFLIGEMITPISEKMAQRMRIKATDSVVAQEFRSGLWVKDGNNFINA
                     EEVLPDTSLLNLHIYEFDENAKLVKIRNAKRGEYSRGEWLLSDVSETNFSANAVEVRQ
                     FAEVNWQSLIRPELLNVLLIMPEKMSALNLYSYIRHLSLNKQKTTRYEVALWAKMIYP
                     LACIVMVLLALPFGFVQQRAGGASNKIFLGIMLGVTYQVLNRVSAHLGLLNDWPPLFS
                     AMMPTVLFLIAALTMLYFVERR"
     gene            complement(12893..13336)
                     /locus_tag="PKL42_00060"
     CDS             complement(12893..13336)
                     /locus_tag="PKL42_00060"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNU65486.1"
                     /translation="MLEHSSSPHLKQAPSLLILGACFIYETLVVAAILFASAFVYLWL
                     FGEATHGLKRYVFQFYLWATVGVYFIWCWHKNGQTLAMKTWRLKLFNQHAQLLSFQLA
                     TLRYVLATVSLLFLGLGFIWAVLDKEHLYWHDRYLKNKLVSIKQS"
     gene            complement(13367..13807)
                     /locus_tag="PKL42_00065"
     CDS             complement(13367..13807)
                     /locus_tag="PKL42_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HNU65487.1"
                     /translation="MVSFKKRFKYLIFFVLLISGQAMAGESTVSWAELNDSQKQVLNP
                     LASEWDTLRPWQREKMLDIARDYPKMDAAKQALVQKRLGNWSRMTPYERENARKSHQQ
                     FQSLSADKKNELRKKWVEYQKLPESECARLRAESPEVYQDADLN"
     gene            complement(13774..14235)
                     /locus_tag="PKL42_00070"
     CDS             complement(13774..14235)
                     /locus_tag="PKL42_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HNU65488.1"
                     /translation="MREMQKKINDVGDVPIDAQDEALVQLAAGLLKDHANHLNATTLQ
                     KLANARGAAVNRLTSRQAGGVNHSGGVLAWFGSEFSAYVEQHKMLSATMVISALLITL
                     FAVQQLSDKNNTGVNSLERSDAFLLASELPPEAFADKGFNTWLVSKKDSNT"
     gene            complement(14245..14847)
                     /locus_tag="PKL42_00075"
     CDS             complement(14245..14847)
                     /locus_tag="PKL42_00075"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNU65489.1"
                     /translation="MATPKELSDFLAEVERRAYKQTVYAVRDDHAALDIVQDAMMKLA
                     EKYGDKPAEEFPMLFQRILQNTMRDFWRRQKVRNLWTTLLSSFGSREDGDDHDPLETI
                     DVEDDSDEPSARLERSQTIRLLEQAIEKLPTRQREAFVLRYWEEMDVAETAAIMGCSD
                     GSVKTHCSRAVHALAIELKKQGLENIGLSGLMLSSDSSKG"
     gene            15004..15255
                     /locus_tag="PKL42_00080"
     CDS             15004..15255
                     /locus_tag="PKL42_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HNU65490.1"
                     /translation="MWRLIFLGLVIWLVVYWVKRHIRSSNKPNATDAQSQSETASNDT
                     SEKVEDMVQCAHCAVHLPRSEAFLVNGKIYCSKNHIPKI"
     gene            15252..16868
                     /locus_tag="PKL42_00085"
     CDS             15252..16868
                     /locus_tag="PKL42_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNU65491.1"
                     /translation="MSILKFSLNSYLNQTVAKQNELPLQVRAVNILNIFRLVLSLIFI
                     GLYLLMSKEIGWHKETDPLFFNLSVSYFIFSLISLFISRLKFAAHPYLLPMQVLVDIL
                     FITLIMHAAGGAQSGLGLLLIISIVTASLVSPGRLALFYAAVASICLLTEQSYQIVIK
                     DLSNSSYTQTVMLCLSCFATAWLAYSLAKRMQQSEALASSRGIDLKNLAQVNALITQE
                     MQDGVVVVNEDLDIRHHNVQAESLLGFTSSNKGSISLNTAAPEIATAFHHWAHIIGAS
                     SETIVTISNRELKLRLMPISQQRDLGAVIFIQDWSLLQAKTQQTKLAALGRLTANIAH
                     EIRNPLSAISHANQLLQEDENSPSTTRVLQIVEDNIGRIDQIIKDVLELNRRDRTHQV
                     DIELGTFLKDFHSQFCAIENISTMHFLLEPTLGEYHVTFDRRHLTQILWNLCKNGWQH
                     SQKQVGSLSLGCVGVGKSGINIQITDDGSGISEQDLPRLFEPFFTTKSTGNGLGLYIS
                     RELAEANGAQLKYQAVPQGSLFLIQLKSST"
     gene            16931..18352
                     /locus_tag="PKL42_00090"
     CDS             16931..18352
                     /locus_tag="PKL42_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNU65492.1"
                     /translation="MTQKHTCLIVDDESDIRELLVMTLERMDIDTYSANGLEEAKTLL
                     AQRPYSLCLTDMQMGDGTGLELVQYINEFHAGLPVAVITAHASADNAVAALKAGAFDY
                     LSKPLSLKQLRPVIEFALKLTPQTSHAPSDTLEMIGDSEPMTHVRHMIAKLSRSQAPV
                     YISGESGSGKELAAKLIHKNSARAEGAFIAVNCGAIPENLMESEFFGYKKGAFTGANQ
                     DKEGMFQAANGGTLFLDEVADLPLPMQVKLLRAIQEKKVRMVGGTAEEAVDVRIISAT
                     HKNLVEMMEAGQFRQDLYYRLNVIQLKMPALRDRPVDIPLLANLLLEKLCKNLGVDAP
                     KIEQSTQDYISNLAFPGNVRELENMLERALAMCDGQTITIDDLMHHEEIRIERRKVPR
                     DEFHKLPSTNPNISPDVKLPEYLEEVEKRAILQALEKTNQNKTAAAKLLGVSFRTLRY
                     RLVKLGLSKEAETALEDELEAEE"
     gene            complement(18532..19536)
                     /locus_tag="PKL42_00095"
     CDS             complement(18532..19536)
                     /locus_tag="PKL42_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HNU65493.1"
                     /translation="MVFATLFLMLIVILIAAEVFTNALEHLGEKLGISEGVTGSLFAA
                     VGTALPETMVPLLALLAGTSSVAVNEEIGVGAILGAPLMLATLSIFLMAFSVWKRRTS
                     IGHLRPEKTGLLRDLNFFIAAFAFATLAMFVPHQHIVVRYGLGFGMVMIYFVYVMLTL
                     RASKDLVEDGHATEADSDMFLCKLGLPNNMVVIIVQLVLGLALLIAGAKGFIHGVEEA
                     ASILGISALLLSLLIIPIATELPEKVNSILWIRKGKDTLAFGNITGAMVFQGTLLPAI
                     GIMLTPWEPRKEVLAGVIITMIAAIWLRVMATREGGLRVWHIGFNGLLYVTYLAIVLL
                     "
     gene            19602..20162
                     /gene="ampD"
                     /locus_tag="PKL42_00100"
     CDS             19602..20162
                     /gene="ampD"
                     /locus_tag="PKL42_00100"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HNU65494.1"
                     /translation="MHLLESSVISISQAGFANNGVYIPSPNQDVRPNHEIALIVIHNI
                     SLPPQQYGGNGIVELFTNQLNPNDHPYYAEIYTQKVSAHFLIRRDGHLIQFVSCLARA
                     WHAGVSNWQGRERCNDFSVGIELEGSDFEPFEDVQYNTLNKLITALTKIYPIQAIIGH
                     SDIAPNRKTDPGPYFDWLKLHHPIQS"
     gene            20167..21264
                     /locus_tag="PKL42_00105"
     CDS             20167..21264
                     /locus_tag="PKL42_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNU65495.1"
                     /translation="MKIYNTLILLAAVAVLAACNKPKNAEEKAARNPPVITTAQVQTQ
                     TLAITEEAIGTLEGLVDPTVTAEIAARVIKVDVSVGQTVKKGQLIALLDATDSGLARQ
                     EAQAEVARIEVLLANQERNVARNQTLVDKNFISKNALDDATAQKNALKQQLQAAKIRV
                     ASASSTNAKTRIYSPADGKVEKKIVDSGEYVRAGDPIVQIIGNQKLRAHIPFPENIAV
                     KIKPGQPIKLSTPTSNTIVNTTVKEIKPLIMADNRSVDVIADVQNVQDWQAGASINAE
                     LTLNEKTGVLMVPEQSVVLRPAGEVVYVIQNNKAFQRIIQAGERKAGMVEVLNGLEAG
                     LEVAVDGAAYLTDKTPVKTSTQQANVEKNLP"
     gene            21261..24389
                     /locus_tag="PKL42_00110"
     CDS             21261..24389
                     /locus_tag="PKL42_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNU65496.1"
                     /translation="MTLPELSIKRHVLAWMISGLLVLFGLIAYGRIGIDRMPNVELPV
                     ISVSTTLRGANPEIMDTSVTNLIESAVNTTPGIEHIQSTSSPGFSNVTITFALEKNID
                     VAFNEVQSKVNQTLRRLPDDADPPVVQKLETNAQPIMWLALHGDRTVQQLNLYAQNVI
                     KKKLENINGVGEVRLGGRRDRTIRVNLNPDRMAAYKITAGDLTTAFQKEHIQLPGGFL
                     VGSHAEQLLKLDLEFHDLKSLAELVISYKSGAPIRLKDVAEIEDGLTDNRQVARFNGK
                     PTIGLGIVKISNANTVAIIDAVNQKLNEDIRPNLPPGLTLDISTNDADFIHEMVQTLQ
                     DHLVEGTLFAALIVLIFMRSFSSTLMICLEIPVSLFGAIAVMYFAGYTFNSMTLLALL
                     LLIGIVVDDAIVVRESILRHMSGEIGTALPLADQNNPQAIRRFRTQATINGSREVVFA
                     VLASTAALVCIFAPVIFMEGMMGKFFKSFAVVVTFGVLVSLLVSLTLTPMLCAHYLKV
                     QPKQNKIYLSLGHFFNRMDLAYHNLLTVVLNHRSLVLVVTLLVVLSSAYFLKNLDKDF
                     VPEMDEGRFMVNIKTPLGSNLNYTESRLKLIEDALAPHRDQIASYFAAIGMGSQGQVN
                     QGMMFVTLKPKEMRSMKQQDIIKSLRKKFDEIPGVRAAPSAISIVRGQRSEKLQFNLT
                     GPNLVQVGALSLALQQKLNETPGMGKVDLDLQLDLPQYVMHVDRVRAASLGITSSDIA
                     NAITVFTGGLDIAEYNDAISDGQRYKIRLKAKDSDFSQIADVNKIYLRTKSGELTRLD
                     SVVTFKQELGAAVIGRYDLQYAANLYSNPSMPLGEASQKVDEAAKNLLPAGYSIGLTG
                     QALEMQKTVKNTAFIFILAFVLLYMVLASQFNSFIQPMIVMLAQPLAIIGGLFALWLT
                     NHSLNIYSMIGLVLLIGLVAKNSILLVDLTNQLREKGTGINEALLEACPVRLRPVIMT
                     SLTLILALFPAALGLGAGAETNGPLAVAVIGGMISSTLLTLVVVPAAYSLVMHGVARF
                     EARSNQPAKSIA"
     gene            24857..26245
                     /locus_tag="PKL42_00115"
     CDS             24857..26245
                     /locus_tag="PKL42_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HNU65497.1"
                     /translation="MSNNQTPIVFKGRTSEYFGIWIVNLLLSIFTLGIYSAWAKVRRK
                     KYFYNSTLIDGVGFDYHANPIAILKGRIIAFVLFVLYQVLAAFSPIAAGVLFVLFLIA
                     LPWIVIRGLMFNARNSSHRGLRFDFDGKYGEAALVFIGYTLLTVVTLGLAIPFVAQRT
                     NQFIVSHHKFGTSHFKMEALVKDFYKIYLVIFLIPLIGILAAVAIPAYQQYVKKAKQA
                     QVQPISQNLFQEINASGFIKVANTDAVEMPAEEIDAAEMATQEAIDAAEAEGYAQDAS
                     EEALLKDLTPEERAEYEKLMKAFEGDAMADDAEGNYDDEALTPMKKDPIEEYFTNLAT
                     TWGVVAAIIIGILAMLLYLAFIFSFIAYFKSRIQNLVLNSTTLDHIGFSSTQRMRDLL
                     WLYISNALVLMFTMGFATPWAQIRMARYRLEHLALVGEADLDKFVGEKKESARALGEE
                     VADFFDVDLSFG"
     gene            26260..27315
                     /locus_tag="PKL42_00120"
     CDS             26260..27315
                     /locus_tag="PKL42_00120"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNU65498.1"
                     /translation="MQFEADYFDGISPRAKRVLVTVRDGHALIIAPVQSAEIAINTLE
                     AALQYEIKHCTVQARLGQGRRLIDLPDDTRLETDYQDLEGCLPAKPSNAFWKGLHYAE
                     SHLGLVAISVVCIVFAGYALLKYGVPVAAKYAAMATPPAIEKDLGKQTLATLDHEKLG
                     YFEATKLPKARQDEIKAALNVLCQKTQNCPEYDLQFRKSPTIGANAFALPGGYLVMTD
                     ELVELAKNNNEITAVLAHELGHVKGRHALRQTLQGTVSGIIIIAITGDASSVAAGLPA
                     LMMNMSYSRELETEADIYSLEALKTACIPTKSFATLLLRLVGSHGGGDMIPEMVSSHP
                     DTKARIKPFLQEQQTCV"
     gene            complement(27351..27872)
                     /locus_tag="PKL42_00125"
     CDS             complement(27351..27872)
                     /locus_tag="PKL42_00125"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNU65499.1"
                     /translation="MSALCFRLARVADAAQISALVNMAYRGEASRQGWTTEADLLDGL
                     RTTTEQVVDIIKTPHSVLLLCLQNEVIVGSICLEHQYETTHVGMFVVRPNLQGKGIGK
                     QLLTYAEDYAKQHFNSNRLMMRVISQRHALIDFYHRRGYQSTGVDEDFPVNAEMWQPK
                     VDGLKLVQLVKLT"
     gene            complement(27881..29119)
                     /locus_tag="PKL42_00130"
     CDS             complement(27881..29119)
                     /locus_tag="PKL42_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HNU65500.1"
                     /translation="MKLPSLNIQILLGAIFGVAIGLYFQHLGLENTVVETGLYTSGLV
                     GTLFIDLLKMILIPLVFCSITVGIANLRQHQQMQRVWKVTLIYFVLTMMLAILLALFS
                     ANLFKPGSGLHITMFQDAMQNFEAKQLPLSEFFAQFLHSLFVNPFSALAQGNVIAVVV
                     FALILGVALVMGGERYTTTLRVLQEVLDITMRIVGWVMRLAPLGIMALLIKLVAMQDV
                     VILTTLAKFVAVVIGTLLVHGLVVLPLVLFVVTKKTPLWFWRGAREALITAFATSSSA
                     ATLPITMRNATQHLHVKPEIAGFVLPLGATINMDGTALYEAVAALFIANLVGIELSFA
                     QQLIVFMTAMIAAMGAPGIPSAGMVTMVMVLQSVGLPAEAIAILLPIDRLLDTLRTTV
                     NVQGDMVGSLVVQKLVDQSH"
     gene            complement(29287..30657)
                     /locus_tag="PKL42_00135"
     CDS             complement(29287..30657)
                     /locus_tag="PKL42_00135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HNU65501.1"
                     /translation="MTPNLIRQAAVMLSNILEFNSPADAKLSEFFRNNRDLGTKERAF
                     VAESVYGVLRRLRFLSTVTANDEHDSDDARKLILAWMLRIQGMSIQKMEPMLNEQQLE
                     WAKAIKAKSTENLPLAVQADVRDWLWDKLVAQYGEKEALTIARSMHESATLDLRVNTI
                     KATRDDVMAKFAAEAATGEGNVTKTPYSQTGLRMPLRLNIGRHALFTEGHIEVQDEGS
                     QILAFLVAPKRGMMVADFCAGAGGKTLAMGAIMRNTGRLYAFDVSEKRLHSLGQRLKR
                     SGLSNLTAQRISSENDQKLKRLNGKFDRVLVDAPCTGLGTLRRNPDLKWRQTPEDLAE
                     LTQKQAAILARAAKLVKAGGRLIYSTCSLLQDENENIAEQFLATHADFKLCDAAEILR
                     TQQIDLDTGNYLKLLPHLHHTDGFFAAVFEKNDANLPVKNDDKLQPEEVTAKKTAAKT
                     KAKKLN"
     gene            complement(30707..31840)
                     /locus_tag="PKL42_00140"
     CDS             complement(30707..31840)
                     /locus_tag="PKL42_00140"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNU65502.1"
                     /translation="MWNNAFIIQLKGLLSDASFRRFVFALGLSLLLHFFLIGRFYFNL
                     PTLAEKPLVIVATLLVPQPPPQLALETPQPPPAPSIDKQKKAIEPKPKPVDAKPDVNS
                     AVEAPSMEIEPATDLGATEVLSPELVAPEASAQIAQPEQIETANDAQTEENPIEINLN
                     AYQKVDAEFDVYTAGEASPSNSPAGSAHMAFERMPNDNTYQIKSLIKARGLAALVIPD
                     LLQTSAGDIGQAGLKPRHYLYQFGNKQNKTFSADFDWEAHQLNLHSVNGSQAKVLADG
                     TQDLLSFMFQFMYVPPLQNMQLNITNGKKVGVYDYSFEGEEVISTKMGELNTVHLLRS
                     SAEKEKTTELWLALDYQYVPVKIRETEKDGKVYVLLVKALKTE"
     gene            complement(31830..32546)
                     /locus_tag="PKL42_00145"
     CDS             complement(31830..32546)
                     /locus_tag="PKL42_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNU65503.1"
                     /translation="MINLIRAFILVSLIFSFSGTSLAADLPKRIQAEYSVTKDGMAFA
                     DVRETFVVTGNTYKIESETKGIGVYALLGVRKLVSTGKVTRTGLKPSHFELHQGKNPK
                     KALFADFDWPKNTLRMQVKGKPREATLTAGTQDLASFAYQFMYLPKPLAKDVIVKLTT
                     GKKLNTYEYSNNEMPSTLNVAGKSYKAIHLVPKNNEKGQVETKELWLAADRHYLLLRF
                     LMVDEHGAKLEQTLTKLHVE"
     gene            complement(32633..33682)
                     /gene="purM"
                     /locus_tag="PKL42_00150"
     CDS             complement(32633..33682)
                     /gene="purM"
                     /locus_tag="PKL42_00150"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNU65504.1"
                     /translation="MSANNSDNTSISYRDAGVDIEAGDALVEQIKPFAKRTMRPEVLG
                     GIGGFGSLFAMPKKFKEPILVSGTDGVGTKLKLAFELNKHDTVGIDLVAMSVNDILVQ
                     GAEPLFFLDYFACGKLEVGTAAQVIKGITEGCEQSGCALVGGETAEMPGMYPAGEYDL
                     AGFAVGCVDKANIIDGTTIAVGDVVLGLASSGAHSNGYSLIRKLIDKSGVDFESDFHG
                     RKFKDVVMAPTRIYVKSILKLIDTLPVKGMAHITGGGITENIPRVLPAGLTAEIKAAS
                     WPLPPLFQWLQAQGNIVPSELYKTFNCGIGMAIVLSSAHVAQAKALLEAAGETVFEIG
                     KIRAQAEGEAPTVVV"
     gene            33807..34871
                     /locus_tag="PKL42_00155"
     CDS             33807..34871
                     /locus_tag="PKL42_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNU65505.1"
                     /translation="MEKRNPMIWWLAGLLVCGLIYLLSPILTPFLVAAILAYIANPLV
                     NKIDAFQFKNLSPSRTVASLLVMCLILAVILLILLIVVPLIQKEVVLLLQKLPAYFNT
                     AKSSLEPWLQKNFGVGIDIDFAQIQQILTENWKTAGNFAKNIAQTLSTKGLVFIGWLA
                     NLVLIPLVLFYLLVDWNILVNKVGQLIPRKYIAKTQEIALEIDAVLAEFLRGQLSVML
                     LMSMFYSAGLWLAGLEVAVPIGILSGLLGFIPYLGFGLGLILGLFSALLQFGNITDII
                     PVLVVFGLGQIVESMVLTPYLVGDRIGLHPLVVIFALMAGGQLFGFAGVLLALPVSAA
                     LAVGFRHAKQTYLTSQFYNG"
     gene            34878..35501
                     /locus_tag="PKL42_00160"
     CDS             34878..35501
                     /locus_tag="PKL42_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HNU65506.1"
                     /translation="MKQLLLDIQPLAPPTLENFIIGKNAEALHSISNALNQQADTRFI
                     YLWGIQGSGKSHLLEACKQIAVTRNLPVSVIDDVHTLDENAQITLFDHFNQLRAGNGI
                     LMTAGNAAPTQMGLRDDLATRLAWGLVYQLHPLTDEEKAQALKTHAKARGMKLPDEVV
                     DYCLRYLRRDLPTLMAVLDALDEWSLTEKKPVTVPMLKKLLQLNLEI"
     gene            35577..37262
                     /locus_tag="PKL42_00165"
     CDS             35577..37262
                     /locus_tag="PKL42_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNU65507.1"
                     /translation="MLRITEIKLPIELADTLIHQDDQIKAALLKRLEIPENELINFSI
                     FKRGVDARKSYAILYVYSLDVEVKHEAKILAKFKKDAHIKPAPDTSYHFVATAPVRPE
                     LVVGLSGSTGSLRTDFKRPIIVGFGPAGIFAALILAQSGFKPIVLERGKAVRERTQDT
                     WGLWRKHQLNPESNVQFGEGGAGTFSDGKLYSQIKDPKHYGRKVIQEFVKAGAPEEIM
                     YVSHPHIGTFRLVGMVEEMRKTITELGGEIRFQSKVEDIEIAQGEVQGVVLQTGERIA
                     THHLILAVGHSARDTFEMVHRKGIYVEAKAFSIGFRIEHPQSLIDQARYGKSYSDDLL
                     TKLGAADYKLVHHAKNGRSVYSFCMCPGGTVVAAASEPNCVVTNGMSQYSRNERNANA
                     GIVVGITPEVDYPAHPLAGMELQRQLESHAFVLGGSNYNAPAQRIGDFLANVPSTKLG
                     EVIPSYTPGVQLTNLDSALPEYAISAIREAIPEFAKQVKGFDLPDGILTGVETRTSSP
                     IRIKRDDDTLQSINTKGLYPCGEGAGYAGGILSAGVDGIKVAEAVALSIVNKG"
     gene            37266..38756
                     /locus_tag="PKL42_00170"
     CDS             37266..38756
                     /locus_tag="PKL42_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HNU65508.1"
                     /translation="MLSRIISLGFLLLLSYASFAAASPAQQMIHAQLEAHPNKSGVYV
                     LDKGEEALLARAWLADHAEKSIEVQYFIWSTDNIGILASEALLRAANRGVQVRVIVDD
                     LLVDAPDKSLLALAKHPNIDIRIYNPQHSVGTPFHKRLLNMAINFRGFNQRMHDKTFI
                     VDGKLAITGGRNMAAEYFDYNQQYNFRDRDALLLGAVVQNMEESFNQFWASLLSAPVE
                     ELYDGLGIIQKHVTVKDAEIQKIYANLHAYAENPENFSPENRKAILETPQAFLKLAEQ
                     IVWTDIEFISDTPGKNKQKFLLGGGGRTTEKLAQLVENAEQQVVIQSPYLVMSNSAIK
                     LFKKAIKRGVKVRINTNSLASTDNIQAFSGYRNQRDILTKMGVEIFEYKPDPQVKQHL
                     LRQAMAANEKPPVFAIHAKTMVVDGETVYIGTFNFDPRSQNLNTEVGVIVRDTALASQ
                     VQNAIETDMHPENSWNAAEDKPDQFASSAKRKRVVFYQLTPIKPLL"
     gene            39019..39207
                     /locus_tag="PKL42_00175"
     CDS             39019..39207
                     /locus_tag="PKL42_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNU65509.1"
                     /translation="MKANVGGIDKILRIVIGAALIAWVLFANGPVWAWIGVVPLATGL
                     FNFCALYSILGINTCKVK"
     gene            complement(39272..40855)
                     /gene="ahpF"
                     /locus_tag="PKL42_00180"
     CDS             complement(39272..40855)
                     /gene="ahpF"
                     /locus_tag="PKL42_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008105898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HNU65510.1"
                     /translation="MALETGIKTQLQTYLAMLREPIVLAASLDDTPAAKEMHALLEEI
                     AGMSDKITLVMEDNARKPSFAVKRGAGHSQAQRAVGVRFAGIPMGHEFTSLVLALLHT
                     GGHPMKLEAEKIAQIAAIEGTFHFETYISLSCHNCPDVVQAMNMMAVINPNITHVMID
                     GALYQDEVAAKEIMSVPTTYLNGAIFGAGRMELDEILLKIDSGAVAREAKKLDALAPY
                     DVLVVGGGPAGASASIYAARKGFRTGIVAERFGGQVMDTLAIENFISVKETDGPKLVM
                     ALEEHVKSYDVEIMNLQRVVAITEPGKEGNKNNDLIELKLESGATLKSKTVILATGAR
                     WRELNVPGEKEYRGKGVAYCPHCDGPLFKGKPVAVVGGGNSGVEAAIDLANFVGHVTL
                     LQFDTELKADAVLQKKLRTLNNVTIVTSAQTTEITGDGHKMHGLTYTDRVTGESKHLA
                     LDGVFVQIGLVPNTDWLKGAVKLSKFNEVEINQHNATSLPGVFAAGDVTTTPYKQIII
                     AMGEGAKAALGAFDYIIRS"
     gene            complement(41030..41593)
                     /gene="ahpC"
                     /locus_tag="PKL42_00185"
     CDS             complement(41030..41593)
                     /gene="ahpC"
                     /locus_tag="PKL42_00185"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HNU65511.1"
                     /translation="MSLINTEVQPFRAQAFHNGKFIEVTDASLKGKWSVIIFMPAAFT
                     FNCPTEVEDAADHYADFQKAGAEVYIVTTDTHFSHKVWHETSPAVGKAKFPLVGDPTH
                     QLTRAFDVHIDEEGLALRGTFVINPEGVIKTVEIHSNEIARDVKETLRKLKAAQFTAS
                     HPGQVCPAKWQEGAKTLTPSLDLVGKI"
     gene            complement(41797..43275)
                     /locus_tag="PKL42_00190"
     CDS             complement(41797..43275)
                     /locus_tag="PKL42_00190"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNU65512.1"
                     /translation="MKLKTIVIATLLATNCLQVAAESSLSGNPYSKSTNFDTSLPDLG
                     DVSQTVFTPLDEQRIGEQIMREVSVSDEVVQDVEVIDYLNALGNKLAAASTDKQQKFN
                     FFVVNDTSINAFAMPGGVIGVHTGLFLAANSESELASVLGHEIGHVTQRHLARMVAAQ
                     KYDTVKNIAGFALALLVARANPELASGAMTTASAVGVQNQLDYTREHEREADRVGLQI
                     ISNAGFDVRGMPAFFKTLQHGSRFSEGGAPSLLRTHPLTGERIADVANRVEQMPYRQV
                     PDSAEFQYVKAKLQAQNGAVETNIAVFEQNIRERRTANEAVEHYGLAVAYLRKNALTQ
                     AEKEVAWLKKNSPPNAMIENLSARVQVAKNNAQQAGLQYAAALKIYPDNRALLYGYAD
                     HFLALKQADNAIQLVKEKQRLYPKDAQFYEVLAKAYTIQNKMLLSHQAQGEAYFRKYD
                     LKRAIEQMELAVKAKDGDFYQHSIVEARLKELQRMQGEDKKG"
     gene            43329..43832
                     /gene="moaC"
                     /locus_tag="PKL42_00195"
     CDS             43329..43832
                     /gene="moaC"
                     /locus_tag="PKL42_00195"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNU65513.1"
                     /translation="MPDSKLNPPQQTLTHFDASGQAHMVDVGDKAVTHRVATASGTIH
                     MQPATLQTIQQGDAKKGDVLGIARIAAIQGAKRTSDLIPLCHPISLTKVAVTFNIDAE
                     NASVTCTATCHTTGQTGVEMEALTAVSVGLLTIYDMCKAIDRGMTMREIKLLEKQGGK
                     SGHWTAS"
     gene            43872..44078
                     /locus_tag="PKL42_00200"
     CDS             43872..44078
                     /locus_tag="PKL42_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65514.1"
                     /translation="MLKLIFICTLSMVTITGCEQQKNASAEVGTVPKQIIDKATQDIN
                     NAQALATENLKALEQLDAPTEAQQ"
     gene            complement(44509..45507)
                     /locus_tag="PKL42_00205"
     CDS             complement(44509..45507)
                     /locus_tag="PKL42_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNU65515.1"
                     /translation="MTHVMIVGGGIAGCMTAMELVTRGYRVTIVERNQIAAQTSGESS
                     WAGAGIVFPLLPWMYRDEVNQLALAGANLYPQVTQQLLRETGIDPELTKCGMRILPPY
                     DLALAQQWCEKNKIEFTHVGDDLLLPNVAQVRNPRLLQALKQWLILHEVTLIEHTQLA
                     PIQETASLNGWQTTKGETLRADLYIVTSGAWSFELLKETAANIDIKPMRGQILLYQMP
                     VNTLQHILYQDGFYMLQRRDGFLLAGSTLEDVGFDANVTETARREMQAKAEAILPALK
                     GMPIHQHWSGLRPGAPENLPTIARHPTIHNLYLNTGHFRYGVTMAPASAKILADLL"
     gene            complement(45504..47489)
                     /locus_tag="PKL42_00210"
     CDS             complement(45504..47489)
                     /locus_tag="PKL42_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HNU65516.1"
                     /translation="MFDTLPQILLLLVSAVLAVALFREFRLPAMLAYFFVGAVLGPHT
                     FGLLPDTEATHELAEFGIVFLMFSIGLEFSLPQLYAMRKKVLGLGGAQVVLTLAIVMG
                     IAKLAGLDWSAAFVVGAALAMSSTAIVSKILAERVDLNSRHGRLSIGVLLFQDLAVVP
                     VLVLIPALGAEGAKLLDVLGMAMLKAAAMLLFLFTVGKWLINPWFNLVASQRSRELFV
                     MNVLMVTLLLAYVTKLAGLSYALGAFIAGMLISETKFRYQVESDISPFRDILLGLFFI
                     SIGMLLNMAQILQHIGLVLLVLVSFVLLKAVIVTLVVRFVKYEIGVAIRTGLILAQAG
                     EFGFVILALGVEQKLIGGEALQVILAASLLSMVIAPLLIQYNGRIARKMARSYTRNSG
                     QVVQDIDDVGKHMHNHVVICGYGRSGQYLARFLIEEHIPYVALDIDPTRVLEAAAAGE
                     NVMFGDAARRAVLEAAGSARAKALVISYADNRAAMKILHIVQENYPHLPVIVRTVDDS
                     NMEALQAAGAAEVVPEILEGSLMLASHALMLMGVPLNKVVKRIRLFREERYKLFKGYF
                     HGISDVEEDALEKQQVRLHSVIISPGSYAIGRRLADMQLENFEVEVKSIRRPNSNGTV
                     PTPESQLAEGDVVVLLGQPIGLTNAQNAMLIGKVATK"
     gene            47724..>48231
                     /locus_tag="PKL42_00215"
     CDS             47724..>48231
                     /locus_tag="PKL42_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNU65517.1"
                     /translation="MPSLPKTQTQVTAKQPTLSLARDVLLLEASEINALANRLDQHFS
                     DAVALILQCKGRVVVSGMGKSGHIGGKIASTLASTGTPAFFMHPAEASHGDLGMITAG
                     DIVIALSNSGESDELLAIITPLKRLGASIIAITGNNASSLALAADIHLSASVSKEACP
                     LGLAPTSSS"
BASE COUNT        13349 a        11294 c        10209 g        13379 t
ORIGIN      
        1 cgatagcaag gtaaactgct ttcgctctga cagcaccggc atcacgtact ttgacgtgga
       61 tgcaatccag atacaccact ggataaaccg catccagtgg ccgtgactgc cattgcttta
      121 catcatccac caccgcatcg gtgacagttg aaataagtgt aggtgatatt tccgtgccat
      181 acatatcggt gaggtgtgcc tggatttcac gcaccgtcat gccgcgtgaa tacagtgaga
      241 tgatcttgtc atcaaagccg gtccaacgtg tttgatgttt ggggataagt tgcggctcga
      301 aactgccttc gcggtcacgg gggacttcaa tcggcagttc accaaactca ccctttaaag
      361 tcttacgact tttaccgtta cgggcattac cgctggcatt ggtgacggct tcatgcttgt
      421 catgaccaag gtgctcagcc atctcagctt gaagcgcacg ttcaactaat gctttggtga
      481 gttgtttgag aagaccgttc tcaccaatca gatcttctgg cttcttgtaa ttcgatagca
      541 ggctatcaat taaatcgttg ggtaaggctt ttggtaacgt catttttaat ccatttctga
      601 aaatagcagt ttcctgcaaa atgacgttta cacaaaatta tttacaccct caggacaccc
      661 tcaggacacc ctctcttttg atcattgtga gttagtcaat aaatattatg ttttcggctt
      721 cttgttttac aataaattca gtttcgtaag attgtttatt tttaggctta aaattagtga
      781 agtcaaataa ctccctgtca agcaagtgcg acggggatac attacaaact gctctaaaca
      841 tactctcaac tcttccagga ttttccttac tccatttacc caaaagctct ttcattactt
      901 gtcgctggag atttggttga gagccacata agttacatgg aattatggga aactgcatca
      961 tttgagaata ttgtttaatg tctttttcaa cgcagtaagc taagggtctg attacaatat
     1021 tattgccttc atcagatcgc agtcgaggcg gcatagcctt taaactacct ccataaaaca
     1081 agttcaacaa taatgtctct aaaatatcat ctctgtgatg tccaagggca attttgcttg
     1141 cccctaactc atttgcagtt ttgtaaagta ttccacggcg cattcttgaa cataatgaac
     1201 acgtcgtttt gccttcagga attttttcct tcaccacact gtaagtatct tgctccacta
     1261 tcttaaaatc aacaccaatt ttcttaagat aagttggcaa tacatccgcc ggaaatcctg
     1321 gttgcttttg atccaagttt actgccacaa ctttgaaatc tacgggagct attttagaaa
     1381 aatatacaag tgtgtctaac attgtataac tatctgctcc accagataaa cacaccatta
     1441 caagatcacc attttcaatc atattaaaat ccatgattgc ctttccagca tccctacgaa
     1501 ttcttttttg caatttgtta aaactatatt gattcattaa tactctctac tttttagttg
     1561 gtttggttca taaaagcaca attctaaatt aaaactaaaa tactgatacc aatttaagat
     1621 gtatcacaac atatcgaagt accaagcgta taaaactttg aatagcatgt ttatgatgta
     1681 agtttactag tcagataatt ttaaagctga gtcttgatta tcagcgataa gtgaaaaatt
     1741 aatatttttt ttgagaattt agggcatcat aaatgtaaag attgttctag tgacagccta
     1801 aaagcttaca tttttatttt atttgtcaac atgattattt attttgtata tgttttaaaa
     1861 ggattatttt aaaaatagct aacatggtta tgctaataag taaagactct cacgccatgc
     1921 tcatgctatt actggcactg caagcgcgcg cgcccattga ctttaagttg attgcgatga
     1981 atcttgatca aaagcaaccc ggttttccgg ctgatatttt gcccgcctat tttgaaaaac
     2041 tgggcgttga ctaccgcatt gttgaagctg acacttactc tattgtgaaa gaaaaaatcc
     2101 ctgaaggcaa aaccacctgc tcgctctgtt cacgattacg ccgtggtgtg atttacacca
     2161 cggccaaaga actcggtgca aataaaatcg cgctgggcca tcaccgtgat gacattgtag
     2221 agacgttatt tttgaatcta ttttttggtg caaagatgaa agccatgcca cctaaacttt
     2281 ccaccaatga taaacaaaac attgtcattc gcccattagc atattgcagc gaaaaagata
     2341 ttgccagcta cgcgcggcaa atggactttc caattattcc ttgcgattta tgtggtagtc
     2401 aagaaaattt acaacgccaa aaagtcaagg aaatgcttca tagttgggag cgcgaacaac
     2461 cgggccgcat taataatatt tttcgtgcca ttggtaatgt tgaaccttct cacttggccg
     2521 actttaatct ttatgacttt aaaaatttaa gccaagccaa acctgaagat gaagatccgt
     2581 tattcggcga catcgccaac gaaggtgcag cactgagcat taataacact gctggcacac
     2641 gcatcgaatt tgttagaaat acataatgtt acaaatattt caataaattt gcgtagtatg
     2701 cgcggtaacg tgttgtcttt tagctattga ccttgctatt atccaagcta caggcaacgt
     2761 agcaccgtat tccagccctt ttaaacattc attttcctag gcttgcatgt ccaaactgtt
     2821 aattgttgaa tccccttcca aggctaaaac gcttaaaaaa tatcttggaa gcgactttga
     2881 ggttttagcc tcatacggac acgtgcgtga tttgattccc aaaaatggcg ctgtagatac
     2941 cgaacacgac ttcgcgatga agtacgatat tatcgagcgc aatagcaaac atgtggacgc
     3001 cattgccaaa gccgtcaaaa gtgcagacag catttacctc gcaaccgacc cggaccgcga
     3061 aggtgaggca atctcttggc atattgcgga gatattaaag tcaaaaaatc tacttaaaaa
     3121 taagctcatg aaacgcgttg tatttcatga aattacaaaa acagcggttg aacacgccat
     3181 tgcggagccg cgtgatattt caatgccaat ggtcaacgcg caacaagcac gccgtgcgct
     3241 cgattacttg gttggcttta atttatcgcc attgttatgg aaaaaaatcc gccgtggttt
     3301 atcggctggg cgcgtgcaaa gcccggcgct gcggctcatt gttgagcgtg agttagaaat
     3361 tgaggctttt aagtctcaag aatattggtc tattcattta gatgcgctca aacacagcca
     3421 cggctttagc gcaaaactta ttcagctcaa ccatgaaaag gtagaacaat ttaccgttat
     3481 caaccacgac cagcaaaatg atattgtcgg taaattatta cttgcaagcg ctggcaaaac
     3541 cactgtttca agggtagaga aaaaacaacg cagccgtagc cctgctgcac ctttcaccac
     3601 ttcaaccctg caacaagaag cggtacgtaa gcttggtttc acaacctcgc gcgccatgcg
     3661 catcgcccag caactctatg aaggcatgga cgttggcaac ggcacagtgg gtttaattac
     3721 ttacatgcgt acagactcat ttagcattgc ggctgaggca attgcgcaaa ttcgtgatta
     3781 tgtgaaaaag aatttcgata ctgaatatct gccaaaaacg gccattgcct acaaaacaaa
     3841 atcaaaaagt gcgcaagaag cacatgaggc gattcgcccg accgatatca accgcacccc
     3901 tgctagcgtg cgccaatact taaccgatga gcagttcaag ttatacgaaa tgatttggaa
     3961 acgggcgctt gcctgccaaa tgtcacctgc caagtttgat gcggtgagtg ttgatttaag
     4021 tgttggcagt gatgctaatt tattccgtgc ttctggccaa acattggtat ttcctggctt
     4081 tatcgcggtg tatatggaag gcactgatga cgaggaagaa gaggccgaag ccaaattacc
     4141 gcaccttgaa actggcgaag tactgaccgt agaaaaaatt tacggggagc aacattttac
     4201 tgagccacca ccacgctatg gtgaagctag cttggttaaa attttagaag aatacggcat
     4261 tggccgccca tcaacttatg ccagcattat tagcacactg caagaccgtg agtatgtgat
     4321 actcgataaa aaacgcttta agccaacaga tgttggtcgg gtggtgaata agttcttaac
     4381 tgagcatttc acaaaatatg tggattacga ctttactgct aatcttgaaa atgaactcga
     4441 tgagattgct gaaggcgacc gcgagtggat accggtcatg gatgaattct ggcatggatt
     4501 taaccagcaa ttactcagca aagccgatgt agaccgccct ggcaacgaat taattgacga
     4561 ggcttgccca aaatgtggca aaccgctaca aaaacaactc agccgctatg gcagttttat
     4621 tggctgtacg ggctataacg acacgcctaa gtgtgactac aaacgcagta tcggtggcga
     4681 agcagcggta ggtgctgacc ccatcatcat tggcagcgac gatgaaactg gcaaagaaat
     4741 tatgctcatg aacggcccat acggcccata tttacagctt ggcgcagtca ttgagggcga
     4801 caaaaagaaa ccgaagcgcg tcagtgtgcc taaagagatt tcactggctg acatgagttt
     4861 agaaaccgca aaaatggtgc tgtcactccc gcgcgattta ggccaacacc cggaaactgg
     4921 caaaaaaatt gtggcaaata ttggtcgttt tgggccatat gtgaatcatg atggtaaatt
     4981 taagtcgatt ccaaaaactg aaagcgtctt tacgatcgac cttgcagggg ctgtcaccct
     5041 gcttgcgcaa gcacatagtg ggccagcacc cattgccgat ttagggccgc atccgtcagg
     5101 cgaaggccgc attgaaatct atgccggacg ctatggcccc tatgttcagc atgcaaaaat
     5161 tcgcgctact ttgccaaaaa gtgtagaacc agaagcgctt actttagaag aagcgataga
     5221 gctgctcaat gctaaagcac tcaaagatgc gcctgctaaa aaaacaacgg caaagaaagt
     5281 agttgctaaa aaaccagcca ccaaaaaaga agccgtaaaa aaagtggcga ctaaaaaatc
     5341 cgtcactaaa aaagctgtta ctaaaaaacc ggtcgctaaa aagccggcta aagaataaat
     5401 tctgaatatt ggtaaaaatt aaaaacggcg cttgcgccgt tttttttatc taacatctgg
     5461 ctaatgacag cattgaaaat taattttaat tcatgctttt tgtgccgagg aaaatttaat
     5521 gccctgagag cctttattta taaggctcac agggcatcga aaaaggttgc agtctaattt
     5581 gttggttttt agccgcttac gtgttcacct agtacttcag ttttattatt ttctacccat
     5641 ttgcgcactc gattggcatc gccaattctt gtagattttc cagccgcgtc cagcaaaata
     5701 ataataagcg attgaccaga aatttcagct tgcatcacca agcaacgtcc cgcttcacta
     5761 atataacccg ttttagatag accaatcatc cattcaccag cccgcaccag cgcattggtg
     5821 ttcacaaaat tcaccgggta atcatggttg agtaaactca tttcttgtga agcggtggtc
     5881 gattcctgac gaatttcatc atacaaataa gcagttttga ccattttgac taaatcttca
     5941 gcggttgaaa cattgttgct gtccaagcca gttgcgtcat aaaacttagt tgaccgcatg
     6001 ccgagcgaag tcgctttgcg attcatagcg cgcaaaaatg catcgatacc acctggataa
     6061 ttatgtgcca gcgctgaagc ggcacgattt tcagatgaca tcaacgccaa acgcaacaat
     6121 tctgcacgcg tgagggcagt gcctacgctt aagcgtgagc gtgtaccttt taaataatca
     6181 atgtcgtctt cagagatata caggacttca tccatcggca gttttgcatc taacactacc
     6241 atcgcggtca tcagcttggt aacagaggca atgggtgtca cttctttggt gtttttttcg
     6301 taaatcactt cgcctgtgta ttgattgatg accaaggcct tggcagaagc taattgcagt
     6361 gggccattgc cgtcatataa atcttcaaag gtttctgtag gttgaacata aacaggttta
     6421 cgcaatttca catgcatgtt tgacttacgt gatgcattta acttactgcc tttaagtttt
     6481 gcactagggc tatgcttagt taacttatat ttgccagtga catttttatt caacgtatat
     6541 tttgcgttgc tgactttagc gttggatgct acattttttt gcttttgttt agacgcagcc
     6601 aatgaagcca ccggggtgag ggctagcatg aaagctaaat aaatgagtgt aaatttacgc
     6661 ataaaaaccc ctaaattttc ttatggtaca tatagttatg tcggaatatt ataattaaac
     6721 tttaactttg tcatcaaata tttacaattt tcccgatgaa cgctataaaa accatcatac
     6781 tttgactaca tctttaattt taacgccaaa ctgggtgttt cggattacaa taaaacctat
     6841 attcacctgc taaattaaaa cttaaattat gtctattcca aaaacactac actacagtaa
     6901 agaacacctt tggacacaag ccgatgttga cggttcatgg caggcaggca tcaccgacta
     6961 tgcgcaggat ttgttaggcg atattgtgtt tgttgatgca ccggcagtcg gcacaaaact
     7021 cgttgcaggc caaccctgcg gcttggttga atcggtgaaa acgggctctg acttacacgc
     7081 gctggtagat ggcgaagtcg ttgcaatcaa tgtagaagta atcaacgcgc ccgagcgcat
     7141 taacgataaa ccttatgagg cttggatttt taaatttaag ccgcttgagg ccaatgttga
     7201 agcgcaactg ctttcaagtg aagattatca gaaactgatt agtgcctagc taccctacgg
     7261 cgcaaggaaa atttcgtgcg ctatcaacca aaattaagcg tatttttaac ggatattgag
     7321 tacaaacctt tttggatgcc ctgagagcct tataaataaa ggcgctcagg gcattaaatt
     7381 tttctcggtg aaaatttaag gttttatttt ggaaaaacca agcgtaccga ttccaccatt
     7441 ttttcaattt ccgctttgat gttagcggtt aaggtttctt caatacgggg cacttctgca
     7501 tctaaaatct catgcacttt gttatcgagc gcctgctcca cttctggcaa ttcggcagtt
     7561 aatccacgct taaattcatt tttggcttca gctgccatcg tcatgtagtc ttcgcgcaag
     7621 cttgcgctca actcattcga caagccttga taaagcgcag ggaaagtatc gcccaacttt
     7681 tgctcaagcg cctgttgcga ttccacctgc agggcatcta aaaacacact gagttctgag
     7741 tgcgtttgtt gtgtcagctg atgatagatg tcggccagtt cggtcggcag gttcgtttga
     7801 cgttcggcaa gaatatcttc atttaattgc gcaattttgc tctgcaaact atctgttgcg
     7861 ttttccaccg tgagggtttc caagctggct agttttgctt gcaattgctc ggccaacccc
     7921 tgctccatgg caggtagggc ttgcgtgagg tttgcctgca ccgttgcaat gccttgtgct
     7981 tgcagttcat ttaacgtttg ttcaatatcg gctttaacaa ttgcggcact gtggtgcaac
     8041 attgccggta attctgttgc caaatccgct ttggttttat ccacaaaatt cgcggtgttc
     8101 gccaataatg attgctctgc gctagtaacc gcctggctta gctcctgatt caccgtttgt
     8161 ttgatgtgcg cggcaagtgt ttgctgcgtt tgctccaact cggtgtaaat cgtttcagat
     8221 aaaaagtcgt ggctagcgcg ttgggcattc gctaattcat tgcttacaaa agattttaat
     8281 gcactttctt tttccagttg aatcgcttca aactgtttat ctaagcgggc taattgctgc
     8341 tcaaattgct gactcaacgc acttgccata taggcctgat tggccttttg aatattcgcg
     8401 gactcattat gcaagttttc taaaatatct tcctgcaatt tttttctgag ttttggtatg
     8461 acagattttt caatctcatc ttctaaatgc ggtctgattt gcgcaataat ttctgcaatt
     8521 aaattggggg ataatggaat cgactttgct gaggatttat ctgcacccgc aatttcagtt
     8581 aatacaggaa tatcatcttc tttcatttcc agtcctttac ttcacctgtt ttttcatctg
     8641 ttgctttaat tgcatagcca cgatctttat aaaacttgaa gcgctgccgt gcagcaattc
     8701 tgtcggattc gtctgtaccc acaatttcca ccaaatgcct aaagcggcta aaaaatggcg
     8761 gatgctgatt ctgtaaatta attaaaatat cgtcttgctg caaattttcg cctgaacaat
     8821 caagcaatat agctgaaaac gcactgtgtg cttcagtagc cttcgcattt gctaaaaaac
     8881 tcgttgcaga atgttgccac aaatactgct gcagcgcgct gttcatttgc tcatcttgcg
     8941 tcaataaagt tagctggcgg cctttattcg tggctttttc gcaaagctca gccgacttgg
     9001 ctaacttatc agccacatta aaatagaact ctacgcgcgt catattgcta atatcacccg
     9061 aaaattattg atttgcgcga ttcactaaaa atgctgttaa aagtggcact ggacgaccgg
     9121 tacccccttt ttctttaccc gatttccagg cagtacctgc aatatccaaa tgcgcccaat
     9181 gatatttttc agcaaagcgt gataagaaac aagcggcggt aatgctccct gcagcacgcc
     9241 cgccgatatt ggctaaatcg gcaaagttac tttccagtaa cggttgatat tcttcccaca
     9301 ttggcatgtg ccaagcgcgg tctaaggccg cctcgcccgc gcgcagtaac tcgtgcgcca
     9361 gcgcgtcttg gttactaaat aagcccgttg catggtgacc aagcgcaatg acacaagcac
     9421 cagtgagggt cgcaatatcg accaccgcac tcggctcaaa acgttcagca taagtcagtg
     9481 catcacacaa aatcaggcgg ccttctgcat cagtattcag cacttcaatt gtcagccctg
     9541 acatacttgt cagcacatcg cctgggcgcg tcgcgcggcc atccggcata ttttcacagg
     9601 ttggaataag cccaatcaca ttcaacttta agtctaacgc tgcaattgtt ttaaaggtac
     9661 ctaacacgct tgcggcaccg cacatatcat atttcatctc atccatttca gcgcctggct
     9721 tcaatgaaat accgccggta tcaaacgtaa ttcctttacc caccagcacg actggttttt
     9781 cgcccacact ccctttgaga tgctgcatga tgataaattt gggaggcgtt tcactgcctt
     9841 gcgacacacc taaaaatgaa cccatgccta atttctcaag cgcctcgcgc tctaacacct
     9901 ctacctgcaa cccatattgc tgacccaacc ccagcgcctg ttctgccaaa taagttggat
     9961 tacaaacatt tgggggcaaa ttacctaaat cttttgccaa actgacccca agcgcaacgg
    10021 cctcaccata gaccaaacct gcatttgctt gagcgatttc actcgtctca gccacgctga
    10081 tatccatgct aagctggata tttttcgtgt ctgcttttac tttctttctt ttgattgcat
    10141 caaattgata gtgcgcatct aatgttgctt caaccagcaa cgccacccta cgctctgcgg
    10201 ttaatttttt aaccgttaat tcagctaaaa aagtggcagc attttttgcg ccacttttca
    10261 ccaaagcttt gagtgaagcg cgcacggcct tgcaatattg cttttctgta aatcctgccg
    10321 ctttacctaa acctaccagc attacgcgcg cacttgcgac atttgctacg tcgtgcagca
    10381 ttaaggtgga ttctaatttg ccatcaaaat caccacgctt taatattttt gtcaaaaaac
    10441 cctcggctac ggcatccacc tgctttgctg cagctgataa ttcttgagat tcgtacaccc
    10501 ctaaaatgac acaatcgtta agctctttcg ccgcactgcc gttttttatg ctaaatttca
    10561 tggtttacaa tcctagtgat ttgatttaag ttaaattatc gcgtgtttta cacgcatttc
    10621 aaagttgtaa ataaactaac cgtgcatata ctgctcttca acatcaatta aaccacccat
    10681 caatatgctt tttaagcgtt ctctcattca agaattaagt gcaagtgctg ctggcgcttt
    10741 tcttatatta acgggcattg ttattgcaca acgtgcaggg tttttaatac gtcacgccgc
    10801 aaaaggcatt atccccaacg acgcaattac caccatgctg ggattcaaca tgattcgatt
    10861 tttacccatg ttgctgtctt taagcttgtt tttagcaatc ctcttaacgc tctcacgctg
    10921 gtatagagac tctgaaatgg tcatttggtt tagcgcgggc ttaagtacca acagcttaat
    10981 tcgccctatt ctcaacttca caatccccat cgtcatcatc atctctttac tgagtttttt
    11041 cgtcacgccg tgggccatca ataaagttga agattatcgc gttcaacttg aaagtcgtga
    11101 cgacttatcc acaattaacc ccggggtttt taaagagtcg gcccatgcgg accgggtctt
    11161 ttttgtagaa agctttgatg aactgggcaa tgttgttaaa aatatcttcg tacaatctat
    11221 tcaacatgaa aaactaggca ttatcgtcgc agcaaaaggc agtcgtcttg tggaaaaaaa
    11281 tggagacaat tttctgctca tgcaaaatgg caggcgttat caaggcgtgc gcggtacggc
    11341 tgaatactca acaacggagt ttgaaaaata cgctattcgc gttgaagccg tagaggcaca
    11401 gcgagatgcg ccaaccaccc agtccatttc aagctttgaa ctcttaaaaa actctaacaa
    11461 tgcaaacatt gccgaactac agtggcgttt atccatgcct attgcagcct tgatactagc
    11521 aatcttggcg attccattaa gcgctgttga cccacgcgcg ggacgctcag ctaacttcgc
    11581 gctcgccatc atcatctata ttttatacat taacttgtta agtattattc aggcatggat
    11641 tgctcaagac aagataagcg gcattgtcgg tttatggccc gtccacttta tttttgggtc
    11701 ggtagccatc tacatggtct atcgccgcat gcagcaactc acattactac ctacatttat
    11761 gattaaggct tggggcaaac ttaaacgcac ttaaccaaaa agcatgaaaa ttattcacca
    11821 ttacttacta aaagaaattg ttgccaacat cctgctcgtg atgttggcac tgattgccat
    11881 gttttccttt tttgatttga ttcaggaatt agacgcgtta ggaaaaggta attatggctt
    11941 agggcaggtg ttattgtttg ttttacttag tgcgccaggc cacgtttacg aggtgatacc
    12001 ggtggccgta ctggttggta cgatgctaac gttaggcctg ctttcacgct actcagaatt
    12061 gataatttta agagtcagcg ggctgtccat cttcaacatt gccgtcatgc tcttaaaaat
    12121 tagcttggtt tttactttaa ttactttttt gataggtgaa atgattacac ctatcagtga
    12181 aaaaatggcg caacgcatga gaatcaaagc aactgattca gtggttgcgc aagaatttcg
    12241 gtcgggccta tgggtaaaag acggcaataa ttttatcaac gccgaagagg tattacccga
    12301 taccagctta ctgaacctcc acatctatga atttgacgag aatgccaaac tagtcaaaat
    12361 acgcaacgcc aagcgcggtg aatattctcg cggagaatgg ctactgtctg atgtgtccga
    12421 aaccaacttc agcgccaacg ctgtagaagt gcgtcaattt gctgaagtta actggcagtc
    12481 tctcattcgc ccagaacttc taaatgtgct actgatcatg ccagaaaaaa tgtccgcctt
    12541 aaacttgtat tcatacattc gacatttaag cctgaataaa caaaaaacaa cccgctatga
    12601 agtggcgcta tgggccaaga tgatctaccc gctagcatgt attgtaatgg tcttattggc
    12661 tctcccattt ggctttgttc aacaacgggc gggtggagca agtaacaaaa ttttcttagg
    12721 tatcatgctg ggagtaactt atcaggtatt aaatcgtgtt tctgcgcatt tagggttgct
    12781 caacgattgg ccaccgctat ttagtgcgat gatgcccacc gtgctgtttc taatagcagc
    12841 gctcacgatg ctctattttg ttgaacgtag gtgattattt acgaattatc actcagcttt
    12901 gtttaatgga taccagctta tttttcaaat agcggtcatg ccaatataga tgctctttat
    12961 ctagcaccgc ccaaataaaa cctaaaccca aaaaaagcaa actcacggta gcgagtacat
    13021 agcgcaatgt tgctaattga aatgataaca actgcgcatg ttgattgaat aactttaatc
    13081 gccatgtctt catggccaat gtttggccgt ttttatgcca gcaccaaatg aaatacaccc
    13141 cgaccgtagc ccacaagtaa aattgaaaca cataccgttt taaaccatgc gtggcttcac
    13201 caaataacca taaataaaca aaggcactgg caaatagtat cgctgccact acaagtgtct
    13261 cataaatgaa acaagcccca agtattaaaa ggcttggggc ttgtttcagg tgagggcttg
    13321 atgaatgctc gagcattatt gtggcgaaat atcaggaaaa ctttatttaa tttaaatccg
    13381 catcttgata cacctcaggt gactcagctc ttaaccgagc gcactctgat tcaggtagct
    13441 tttgatattc cacccatttt tttcgtaatt catttttttt atctgctgac agtgattgaa
    13501 attgctggtg gctctttctt gcattttcac gctcataggg tgtcatacgg ctccaattac
    13561 ccaagcgctt ttgcaccaaa gcctgctttg cagcatccat tttcggataa tcgcgtgcaa
    13621 tatccagcat tttttctcgt tgccacgggc gaagtgtgtc ccattctgaa gcaagtggat
    13681 ttaatacttg tttttgtgaa tcgtttaatt ctgcccaaga aaccgtactt tcaccagcca
    13741 ttgcttgccc actgatgagc aagacaaaaa atattaagta tttgaatctt tttttgaaac
    13801 taaccatgta ttaaacccct tatcggcaaa tgcttctggc ggcaattcag aagcaagcaa
    13861 aaacgcatca ctacgctcca atgaatttac gccagtatta tttttatcac tcaactgctg
    13921 cacagcaaac aacgttatta gtaacgcact tatcaccata gtagctgaca acattttatg
    13981 ttgctcaaca taggcgctaa attcagaccc aaaccaagct aacacgccac cactgtggtt
    14041 cacaccacca gcctgcctag aagtaaggcg atttaccgca gcacctctgg catttgctag
    14101 cttttgcaag gtcgtggcgt tcaaatgatt ggcatggtct tttaacaaac cagccgccaa
    14161 ctgcacaagc gcttcatctt gtgcatcaat gggtacatcc cctacgtcat taattttttt
    14221 ttgcatttct ctcatcgtat actcctagcc ttttgagcta tcgctactta acattaagcc
    14281 tgataggccg atattttcta atccttgttt ttttaattca attgccagcg catgtacagc
    14341 gcgtgaacag tgcgttttaa cgcttccgtc cgaacagccc ataatggccg ccgtttcagc
    14401 tacatccatc tcctcccagt aacgcaacac aaaggcttca cgttgacgcg ttggtagctt
    14461 ttctatcgct tgctcaagca gtcgaatggt ttggctgcgt tcaagccttg ctgaaggctc
    14521 atcgctatca tcttctacat ctatggtctc aagaggatca tgatcatcgc catcttcacg
    14581 actgccaaaa gatgataaca aagtagtcca taaatttcgt actttttggc gtcgccaaaa
    14641 gtcgcgcatg gtgttttgta aaatgcgctg aaagagcatt ggaaactctt cggcaggttt
    14701 atcaccatac ttttcagcaa gcttcatcat ggcatcttgt acaatatcaa gcgcggcatg
    14761 atcatcccgc acggcataca ccgtttgctt gtaggcgcga cgttctacct ctgcaagaaa
    14821 atcagaaagc tcttttggag ttgccataat gtttgttatc aatgcttatt gcgaatactt
    14881 tcaaaatcta cgactctatt gttgacttaa cctgtcagcc aatcgcagta tatcaggttt
    14941 atattttgca ctaagcgacg ctttagatac aatcatacca attcaataca caaagtatac
    15001 cccatgtggc gactgatttt tttaggcttg gtgatttggc tggtggtcta ttgggtgaaa
    15061 cgtcatattc gttcatccaa taaacctaat gcaactgatg cacaaagtca aagcgaaact
    15121 gctagtaatg acacttcaga aaaagtggaa gacatggtgc agtgcgcaca ttgtgctgtg
    15181 catctaccaa ggtcagaggc ctttttagtc aatggtaaaa tatattgcag caaaaatcac
    15241 atacccaaaa tatgagtatt ttaaaattta gcctaaattc ttatttaaat caaactgttg
    15301 caaaacagaa tgagctgccg ttacaagtac gcgccgtcaa tattctcaat attttcaggc
    15361 ttgttttgag ccttattttt attggccttt atctgttaat gagtaaggaa attggctggc
    15421 acaaagaaac tgacccgtta ttttttaatt tatccgtcag ctattttatt ttcagcctca
    15481 tctcactctt tatcagcaga ctcaaatttg cggcgcaccc atatttactg cccatgcaag
    15541 tattggtcga tattttgttc attacgctca ttatgcatgc ggcaggcggc gcacaaagcg
    15601 gcttggggtt attgctgatt atcagtattg ttactgcaag cctggtaagc cctggccgct
    15661 tagccttgtt ttatgcggcg gttgctagca tttgcttact tactgagcaa agttatcaaa
    15721 ttgtcatcaa agatttatct aacagcagct acacgcaaac cgtaatgctg tgcctgagct
    15781 gttttgcaac ggcctggctg gcttatagtt tagccaaacg catgcaacaa agtgaagcgc
    15841 tggcttcatc acgcggcatt gaccttaaaa acctagcgca agtgaatgcc cttatcaccc
    15901 aagaaatgca agatggcgtc gttgtggtca atgaagattt ggatattcgt caccataatg
    15961 tacaagcgga atctttatta ggctttacta gcagcaataa agggtcgatt tcactcaata
    16021 ccgctgcccc ggaaattgcc acggcatttc accactgggc gcatattatt ggcgcaagta
    16081 gcgaaaccat tgtgacgatt agcaaccgcg agctcaaatt aagattgatg ccaattagcc
    16141 agcaacgtga tttgggcgcg gtgattttta ttcaggattg gagtctgctg caagccaaaa
    16201 cccagcaaac caagttggcg gcactgggac gattaaccgc caacattgcg catgaaattc
    16261 gcaacccgtt aagtgctatt agccatgcca atcaactgtt gcaggaagac gaaaactcac
    16321 cttcaaccac acgtgtgcta caaattgttg aagataacat tgggcgcatc gaccaaatta
    16381 ttaaagacgt gcttgaactc aatcgacgcg accgcaccca tcaagtggac attgaattgg
    16441 gaacattttt aaaagatttt catagtcaat tctgcgctat tgaaaacatt tcaaccatgc
    16501 actttttgct ggaaccgacc cttggtgaat atcacgtgac ttttgaccgc cgccatttaa
    16561 cccagatatt gtggaacctg tgcaaaaatg gctggcaaca tagccaaaaa caagtaggca
    16621 gcttatcact tggctgtgta ggtgtcggta aatctggcat taatattcaa atcaccgatg
    16681 acggtagtgg tatttcagag caggatttac cgcgattatt tgaacccttt tttacgacca
    16741 aatcaacagg taacggtttg gggttatata tttcacgtga actggctgag gccaacggtg
    16801 cacaacttaa ataccaagcg gtgccgcaag gcagtttatt tttaattcaa ttgaaaagtt
    16861 ctacttagcg caagcgcgta aaaaaatgtt tcattgttat cgttttaatt aaaacaaggc
    16921 ataatatcgc atgactcaaa aacacacttg ccttattgtc gatgatgaat cagatattcg
    16981 cgaattgctc gtgatgacgc ttgagcgcat ggatattgac acctatagcg ccaacggtct
    17041 agaggaagca aaaacattac ttgcgcagcg cccctactcg ctctgcctga ccgatatgca
    17101 aatgggcgat ggcactgggc ttgaactggt gcaatatatc aatgagtttc atgctggtct
    17161 acccgtcgcg gtgattaccg cacatgcaag tgccgacaac gccgtggccg cgctcaaggc
    17221 tggggctttt gattatttaa gtaagccgct gtccctcaag caactgcgcc ctgtcattga
    17281 atttgccctc aaactcaccc cgcagaccag ccacgcgcct tcagacacct tagagatgat
    17341 tggcgattct gagccgatga cgcatgtacg ccacatgatt gcgaaacttt cacgcagtca
    17401 ggcgcccgtg tacattagtg gagaatcagg tagcggcaaa gagcttgcgg ccaaacttat
    17461 tcataaaaac agcgcgcggg ctgaaggcgc ttttattgcg gtcaactgtg gggcgattcc
    17521 tgaaaattta atggaaagtg aattttttgg ctacaaaaaa ggcgcattca ctggggcaaa
    17581 ccaagacaaa gaaggcatgt ttcaggccgc caatggcggc acactgtttt tagatgaagt
    17641 ggccgattta ccattgccca tgcaagtgaa attattacgc gccattcagg aaaagaaggt
    17701 ccgcatggtg ggcggtaccg ctgaagaagc ggtggatgtg cgcattatca gtgccacaca
    17761 caaaaacttg gttgaaatga tggaagctgg ccaattcagg caagatttat actaccgcct
    17821 gaatgtgatt cagctcaaaa tgcccgcgct gcgtgatcgg cctgtggata ttccgctatt
    17881 ggcgaactta ttacttgaaa aactttgcaa aaacctaggt gttgatgcac caaaaattga
    17941 gcaatccacg caagattaca tttcaaatct ggcttttcca ggcaatgtgc gtgagttgga
    18001 gaatatgctt gaacgcgcgc tcgccatgtg tgacggacaa acgattacca ttgatgactt
    18061 gatgcaccat gaagaaatcc gtattgagcg cagaaaagta ccgcgtgatg aatttcataa
    18121 actgccttcg accaacccaa atatttcgcc tgatgtgaaa ctgccggagt atcttgaaga
    18181 agttgaaaaa cgcgctattt tgcaggcgct tgaaaaaacc aatcaaaaca aaacggctgc
    18241 cgccaaatta ttaggtgtga gctttcgaac cttgcgttat cgtttggtaa aacttgggct
    18301 ttcaaaagag gccgaaaccg cgttagaaga tgagttagaa gctgaagaat aggctggcgc
    18361 acgccgcact ttttttcaaa aaattccttc ttaaaaatgc ttatatttta agcaaaattt
    18421 cctcgagaaa aaatttatgc tctgcgagcc ttataaataa aggctcgcag agcatcgaaa
    18481 aaggttgtgc gataaattgc ttaaatttta aatgctttat ctcgctgaaa actacagtaa
    18541 aacaatcgct aaataagtca cataaagcaa gccattgaag ccgatgtgcc aaacacgcaa
    18601 accaccttca cgcgttgcca tcacccgcag ccaaatggcg gcaatcatgg taataattac
    18661 cccggccaac acttctttgc gcggctccca aggcgtgagc ataatgccga tggctggcaa
    18721 taatgtaccc tgaaacacca ttgcgccagt aatgttgcca aacgccagtg tgtctttacc
    18781 tttgcgtatc cataaaatgc tattcacttt ttcgggcaat tctgtagcga ttggaataat
    18841 caataatgaa agtagcaaag cagaaatgcc aagaatagag gccgcttctt ctacgccatg
    18901 aataaagcct ttggcgcccg caatcaacag cgctaaaccc aataccagtt gcacaataat
    18961 gaccaccata ttattgggca gccctaattt acataaaaac atatcgctat cggcttcggt
    19021 ggcgtggccg tcttcaacca aatctttgga tgcgcgcagc gtgagcatga catacacaaa
    19081 ataaatcatg accatgccaa aacccagccc atagcgcacc acaatgtgct gatgcggcac
    19141 aaacatcgcc aaggttgcaa aggcaaaagc ggcaataaag aaatttaaat cgcgtagcaa
    19201 gcccgttttt tcaggacgaa gatgaccgat tgatgtacgt cgcttccaaa cagaaaatgc
    19261 catcaagaaa atagacaagg tcgctaacat caacggcgcc cccaaaattg cgcccacgcc
    19321 aatttcttca ttgaccgcaa cgctggatgt accggccaat aacgccaata gcggcaccat
    19381 ggtttctggt agtgcggtgc ccactgcggc aaacagcgag cccgttaccc cttctgaaat
    19441 acctaatttt tcacccaaat gctcgagcgc attggtaaac acttctgctg cgattagaat
    19501 cacaatgagc attaaaaata atgtcgcgaa aaccatagct tgcctttagc ctaattatta
    19561 acacgcgcat tttacaggag tttgctagaa ttaccgcaac tatgcattta ctagaatctt
    19621 ccgtcatttc aatcagtcaa gctggctttg ccaataacgg ggtttacatc ccctctccca
    19681 accaagatgt gcggccaaac catgaaattg cgctcattgt gattcacaat atcagcctgc
    19741 cgccccagca atatggcggc aatggcattg tcgagctatt taccaaccag ctaaacccaa
    19801 atgaccaccc ctattatgct gaaatctaca cgcaaaaagt ttcagcccat tttttgattc
    19861 ggcgtgatgg ccacctcatt cagtttgtgt catgtttggc gcgcgcctgg catgctggtg
    19921 tatcgaattg gcaaggtcgg gaacgttgta acgatttttc ggtcggaatc gagttagaag
    19981 gctctgactt tgaacccttt gaagacgtac agtacaatac acttaataaa ttaattacag
    20041 cgctcacgaa aatctacccc atacaggcga ttatcgggca ctcagatatt gccccaaatc
    20101 gaaaaacgga ccctggcccc tattttgatt ggttaaaatt gcatcatcca attcaaagtt
    20161 aaaaaaatga aaatttacaa cacgttaatc ttgttggctg cagtagcggt actcgccgct
    20221 tgcaataaac caaaaaatgc cgaagaaaaa gccgcgcgca acccgcccgt tattaccaca
    20281 gcgcaagtgc aaactcagac gcttgccatt acagaagagg ccatcggcac tttagaaggt
    20341 ttggttgacc ccaccgtgac tgctgaaatt gccgctcggg tcattaaagt agatgtgagt
    20401 gttggccaaa ccgttaaaaa aggccagctg attgccctgc tggacgccac cgattccggc
    20461 ttggcacgcc aagaagcgca agccgaagtg gcccgaattg aagtattact tgctaatcaa
    20521 gaacgcaacg tggcacgcaa ccaaacattg gtggataaaa attttatttc taaaaatgcg
    20581 ctcgatgatg ccacagccca gaaaaacgcg ctaaaacagc aacttcaggc ggccaaaatt
    20641 cgcgtggcga gtgcgagtag caccaacgct aaaacacgaa tttattcacc cgccgatggc
    20701 aaagtagaaa agaaaatcgt ggactctggc gagtatgtgc gtgctggcga cccgattgtg
    20761 caaattatcg gcaaccaaaa attacgcgcg catattcctt ttcctgaaaa cattgcagtt
    20821 aaaattaagc ccggtcagcc aattaaactg agtacgccaa cttctaatac catcgtcaac
    20881 accacggtaa aagaaattaa accactcatc atggcggata atcgctcggt cgatgtgata
    20941 gcggatgtac aaaacgtgca ggactggcaa gcaggggcga gcatcaatgc ggaactaacc
    21001 ttgaacgaaa aaaccggcgt gttgatggtg ccagaacaaa gcgtggtgct acgccctgcg
    21061 ggtgaggtgg tttacgttat tcaaaataac aaagcgtttc agcgcattat tcaagcgggt
    21121 gaacgcaagg ctggcatggt tgaagtgctt aatggtcttg aagcaggatt agaagtggcc
    21181 gtggatggcg ccgcttattt aacggacaaa acgcctgtca aaacttctac ccaacaagcc
    21241 aatgttgaga aaaacttacc gtgaccctac ctgagctttc cattaagcgc cacgtactcg
    21301 cgtggatgat ttcaggatta ttggtgctat ttggcctcat tgcctatggc cgcattggga
    21361 ttgaccgcat gcccaatgtg gagttacccg ttatttcagt cagcactacc ttacgtggtg
    21421 ccaatcctga aattatggac accagcgtca ccaatttaat cgagtccgcc gtcaatacca
    21481 cacccggcat tgaacatatt caatccactt cctcgcctgg tttttctaac gtcactatta
    21541 cttttgcgct cgaaaaaaat attgatgtgg cgtttaacga ggtgcagtcg aaggtaaatc
    21601 aaaccttacg ccgattaccc gatgacgcag acccgcctgt tgtacaaaag ctggaaacca
    21661 atgcccagcc catcatgtgg ctagccctgc atggcgaccg cacggtgcag caattaaacc
    21721 tatatgcaca aaatgtcata aaaaagaaat tagaaaacat caatggcgtg ggtgaagtgc
    21781 gccttggtgg cagacgtgac cgcacaatcc gtgtgaactt aaaccctgat cgtatggcgg
    21841 catacaaaat tactgcaggc gatttaacca cggcctttca aaaagagcat attcaattgc
    21901 ccggtggttt tttggtgggc agccatgctg agcaactgct taagctcgat ttggaatttc
    21961 atgacttaaa atcgctggct gagttagtca ttagctacaa aagtggcgca cccattcggc
    22021 tcaaagatgt ggctgaaatt gaagatggac ttactgataa ccgtcaagta gcacggttta
    22081 atggcaaacc gaccattggc ttgggtattg ttaaaatctc taacgcaaac acggtggcga
    22141 ttattgatgc cgtaaatcaa aagttaaatg aagatattcg cccgaatctg ccacctggat
    22201 taaccctcga tatttcgacc aatgatgctg attttattca cgaaatggtg caaacactgc
    22261 aagaccattt ggtggaaggc acactgtttg ccgcgctgat tgtgctaatt tttatgcgct
    22321 cgttcagttc aacgctgatg atatgcttgg agattcctgt ttcacttttc ggcgcgattg
    22381 cggtcatgta ttttgctggt tacaccttta acagcatgac gctgctcgcc ctgcttttac
    22441 tcatcggcat tgtggtggat gacgccattg tggtgcgcga aagtatattg cggcatatgt
    22501 ctggtgaaat tggcacagcc cttccgctag ccgatcaaaa caatccgcaa gcgatacgcc
    22561 gttttcgcac gcaggccacg attaatggta gccgagaagt agtatttgcc gtgctggcct
    22621 caaccgctgc gctggtgtgt attttcgccc ctgtgatttt catggaaggc atgatgggca
    22681 aattttttaa atcgtttgcc gttgtcgtca cctttggtgt attggtgtcg ctgcttgtct
    22741 ccctcacgct tacaccgatg ctgtgcgcgc actacctaaa agtgcaaccc aaacaaaata
    22801 aaatttactt aagcttgggc cattttttca atcgcatgga tttggcctat cataatcttt
    22861 tgacggtggt gctaaatcat cgctcgctgg tgcttgttgt aacactcttg gtggtgctat
    22921 ccagtgctta ttttttaaaa aatctagata aagattttgt gccggaaatg gacgaaggcc
    22981 gcttcatggt taacatcaaa acgccactgg gttctaacct caattacaca gaatcacgac
    23041 tgaaactgat tgaagacgca ttagcgccgc atcgcgacca aattgccagc tactttgcgg
    23101 ccattggcat gggtagccaa ggccaagtga atcaaggcat gatgtttgtg acgctcaagc
    23161 ctaaagaaat gcgcagcatg aaacaacaag acattattaa atcgttgcgc aaaaaatttg
    23221 atgagattcc tggcgtgcgg gcagcgccca gtgccatttc gattgtgcgc ggccagcgct
    23281 cagaaaagct gcaatttaat ttaactgggc caaatctagt gcaagttggc gcactttcgc
    23341 ttgccttgca acaaaagctc aatgaaacgc cgggcatggg taaggtagat ttagacttgc
    23401 agctcgattt accgcaatat gtgatgcatg tagaccgcgt gcgcgcagca agcttgggca
    23461 ttacctcaag tgacattgcc aacgccatta cggtatttac cggtgggttg gatattgccg
    23521 aatataacga tgcgattagt gatgggcaac gctacaagat tcgccttaaa gctaaagata
    23581 gcgatttcag ccaaattgcc gatgtaaaca aaatttatct gcgcacaaaa agcggagaac
    23641 tcacacgact cgattctgtt gtgacgttca aacaagaact aggggcagcg gtaattggcc
    23701 gttatgacct gcaatatgcg gctaacttat acagcaaccc cagcatgcca cttggtgaag
    23761 caagccagaa agtggatgaa gcagcaaaaa acttgttacc tgctggttat agcattggcc
    23821 tcaccggtca ggccttagaa atgcaaaaaa ccgttaaaaa tactgcgttt attttcattt
    23881 tggcatttgt gctgctctac atggtactgg ccagccagtt caactcgttt atacagccga
    23941 tgattgtgat gctggcgcag ccactggcga ttattggcgg gctatttgca ctttggctca
    24001 ccaatcattc gcttaatatt tattcaatga ttggcttagt gctgttaatt ggcttggtgg
    24061 caaaaaattc gatcttactg gttgatttaa caaatcagct acgcgagaaa ggcacgggca
    24121 tcaatgaagc gcttttagag gcctgtccgg tgcggttgcg gccagtcatc atgacttcac
    24181 tgactctgat tttagcgcta tttcctgccg cactgggttt aggtgcaggg gcagaaacca
    24241 atggcccact cgctgttgcc gtgataggcg gcatgatttc atccacatta ctcacgctgg
    24301 tggtggtgcc tgccgcgtat tctttagtga tgcatggcgt ggcgcgtttt gaggcaaggt
    24361 ccaatcaacc cgcaaaatct attgcgtaat caccgcaatt ttcacgctaa taaaaacatt
    24421 aatacaaagg caaaaactcc cgagaaaaaa ttactgccct gcgagccttt gtttataagg
    24481 ctcacagggc atcgaaaaag gttgttgcca aaataagcgc gtaaacagcc ttttttacaa
    24541 tattacgcat ttctcagtgt aaaaattcac gcaatcaacc tgtcagtttt ggaatattgc
    24601 attcaggtgt tatgattctg cgagaaattt gtagttaagg aagcacttca tgcgtgagcg
    24661 agcgggatga agtgcgaggc gtacggcgcg cagaaaccga gatagtacaa gttgtacagc
    24721 gaggcttcga gcaccgcgca acgaagcagt tcgcccgcgt agccacgcat gaagtgtttc
    24781 ctaaatttca cggatgacca catcctcctt atcaataatt aaaaataact ggcctaggtt
    24841 atctaaggga ttaaacatgt caaataatca gacacccatc gtgtttaaag gtagaaccag
    24901 cgagtatttc ggcatttgga ttgttaacct tttactgtca atttttacgc tcggcattta
    24961 ctcagcctgg gctaaagtac gccgtaaaaa atatttttat aacagcacgc taattgacgg
    25021 ggttggcttt gattaccacg ccaacccaat tgccatttta aaaggccgca ttattgcctt
    25081 tgttttattt gtgctctacc aagtgctggc tgcatttagc cccattgcgg ctggggtgtt
    25141 atttgttcta ttcttaattg cattgccatg gattgtgatt cgcggattaa tgtttaacgc
    25201 gcgcaactca agccaccgcg gcttacgttt tgattttgat ggtaaatatg gtgaagcggc
    25261 attggtattt attggctata cgcttttaac tgtggtcact ttaggtttgg ccattccatt
    25321 tgtggcgcag cgcaccaatc aattcatcgt cagtcaccat aaatttggta ccagccattt
    25381 taaaatggaa gcgctggtca aagattttta caaaatttat ctcgttatct ttcttattcc
    25441 actgattggt attttggcgg ctgtggccat tcctgcgtat cagcaatatg ttaaaaaagc
    25501 gaaacaagca caggttcaac ctatttcgca aaatctattt caagaaatca atgcgagtgg
    25561 atttattaaa gtggccaata ctgatgcggt tgaaatgccc gcagaagaaa ttgacgcagc
    25621 tgagatggcg acgcaagaag ccatagacgc ggcggaagct gaaggttacg cacaagacgc
    25681 ctctgaagaa gcgctgctta aagacttaac gcctgaagaa cgtgcggagt acgaaaaatt
    25741 gatgaaagcg tttgagggtg atgcgatggc cgacgatgcc gaaggcaatt atgatgatga
    25801 agccttaacg cctatgaaaa aagaccccat cgaggaatat tttaccaacc tagcaaccac
    25861 ttggggcgtg gttgcggcca tcatcatcgg cattctggca atgttgctct atctggcttt
    25921 tattttctct ttcatcgctt atttcaaatc acgcatccaa aacttggtgc taaatagcac
    25981 cacgcttgat catattggtt tttcaagcac gcagcgcatg cgcgacttgc tttggctgta
    26041 tatttcaaac gcgctggtgt tgatgttcac catgggattt gccacgcctt gggcgcaaat
    26101 tcgcatggca cgctatcgcc tagaacactt ggcccttgtt ggcgaggctg atttagataa
    26161 atttgtcggt gagaaaaaag aatctgcgcg cgcattgggt gaagaagtag cagacttttt
    26221 tgatgttgac ctttcattcg gttaaacccc gcccaactta tgcaatttga agcggattat
    26281 tttgacggca tcagcccccg cgccaagcgg gtgctggtaa cggtgcgtga cgggcatgca
    26341 ctcattatcg cgccagtcca atctgccgaa attgcaatca atacgcttga agcagcgctg
    26401 cagtatgaaa ttaagcattg caccgttcag gcgcgtttag gccaaggacg gcggctgatt
    26461 gacttacccg atgacacgcg tcttgaaacc gattaccaag acttggaagg ttgcctgcca
    26521 gcaaaaccaa gtaatgcttt ttggaaaggc ttacattacg ctgaatcgca cttgggttta
    26581 gtggccattt cggtggtttg tattgttttt gctgggtatg cacttttaaa atatggcgta
    26641 ccggtggcgg caaaatatgc cgccatggct accccgccgg ccatcgaaaa agacttaggc
    26701 aagcaaacac ttgccacctt agatcatgaa aaacttggct attttgaagc cacaaaacta
    26761 ccaaaagcaa ggcaagatga aatcaaagcc gccctgaatg tattatgcca aaaaacgcaa
    26821 aactgccctg aatacgattt gcaatttaga aaaagcccaa cgattggcgc caatgctttt
    26881 gccctgcctg gcggctattt agtgatgacc gatgaactgg ttgagctcgc taaaaataac
    26941 aatgaaatta ccgccgtatt agcccatgaa cttggccacg taaaaggccg ccatgcacta
    27001 cggcaaacct tacaaggcac ggtgtctggc attattatca ttgccatcac aggcgatgcg
    27061 agtagcgttg ctgctggttt gcctgcgctg atgatgaaca tgagttactc acgtgagcta
    27121 gaaactgaag cggatattta ttcacttgaa gcgcttaaaa cagcttgcat tccaaccaaa
    27181 tcatttgcaa ctttattgct tagattagtt ggcagccacg gcggcggcga catgattcct
    27241 gaaatggttt cctctcaccc tgataccaaa gcacggatta aacccttttt acaagaacag
    27301 caaacctgcg tttaaagatt gatatccagc ttgccgttct gacacccgct ttaagtcagt
    27361 ttgacaagct gcactaactt taggccatct actttcggct gccacatttc agcattcaca
    27421 ggaaaatcct catcaacgcc tgtactctga tagccgcgtc gatgatagaa atcaatcagt
    27481 gcatggcgtt gcgaaatgac tcgcatcatc aatctattag aattaaagtg ttgtttggca
    27541 taatcttcag cgtaagtaag taattgctta ccaatacctt ttccttgcag attggggcgc
    27601 accacaaaca tgccgacatg tgtcgtttca tattgatgct ctaagcaaat cgagccgacg
    27661 ataacttcgt tttgcagaca caacaggagg actgagtgtg gggttttgat gatatcaacg
    27721 acctgctcgg ttgtcgttcg caaaccgtcc agcaagtctg cctctgtggt ccagccttgc
    27781 ctgcttgcct caccgcgata ggccatatta acaagggctg aaatttgcgc tgcatctgca
    27841 acccttgcta ggcgaaaaca caatgctgac atcgctaaat ctagtgagat tgatcgacta
    27901 atttttgcac cactaaactg cccaccatgt cgccttgtac atttaccgtg gtgcgcaatg
    27961 tatcgagcag ccgatcaatc ggcaacaaaa tggcaatcgc ttctgcgggc aatcccacag
    28021 attgcagcac catgaccatg gtcaccatgc cagcactggg aatgcccggc gcacccatcg
    28081 cagcaatcat ggcagtcata aacacaatca attgctgcgc aaaacttaac tcaataccca
    28141 ccaagtttgc aataaataac gctgcgacgg cttcatataa agccgtgcca tccatattga
    28201 tggtggcacc cagtggcaat acaaaacctg caatttcagg ctttacatgt aaatgttgcg
    28261 tggcattgcg catggtaatg ggcaatgttg ctgcgcttga gctggtggca aaagccgtga
    28321 tgagcgcctc gcgcgcgccg cgccaaaacc acaaaggtgt ttttttcgtc acaacaaaca
    28381 ataccaatgg cagcaccacc aagccatgca ccaataacgt accgatgacc accgcgacaa
    28441 acttcgctaa ggtcgtgaga atgaccacat cttgcatggc aaccagctta attaacagtg
    28501 ccataatgcc cagcggagcc aagcgcatca cccagcccac aatacgcatc gtaatatcta
    28561 acacttcttg cagcacgcgt agggttgtgg tgtaacgctc accgcccatt accagtgcca
    28621 cgcctaaaat cagcgcaaat accaccaccg caatcacatt gccttgcgcc aatgcgctaa
    28681 atggattaac aaacaagctg tgtaaaaact gtgcaaaaaa ttcagacaaa ggtaattgct
    28741 tggcttcaaa attttgcatg gcatcttgga acatcgtaat atgcaagccg ctgcccggct
    28801 tgaaaagatt tgccgaaaat aatgccaata aaattgccaa catcatcgta agcacaaaat
    28861 aaatcagggt caccttccaa acgcgctgca tttgctggtg ctgccgcaaa ttggcaatgc
    28921 ccactgtaat tgagcaaaat accagtggaa tcaaaatcat tttcagcaag tcaataaaga
    28981 gcgtacccac caatcctgat gtataaaggc ccgtttccac caccgtattt tctaagccta
    29041 aatgctgaaa atagagtccg attgccaccc caaaaattgc acccaataat atttgaatat
    29101 ttaagctagg aagtttcatt ctggtgacct tttagcggca gtaaatcatg taaataaata
    29161 ttattttaga tgcaaaaaac gctcgagaaa atatttctgc tctgcgagcc tttatttata
    29221 aggctcacag agcatcgaaa aaggttgtgg tgaaaaacag gctaatttaa agctctttta
    29281 cctcaatcaa ttaagttttt tagccttagt ttttgcggca gtttttttgg ctgtcacttc
    29341 ttcaggctgc aatttatcgt cattttttac aggtaaattt gcatcatttt tttcaaaaac
    29401 agccgcaaaa aaaccatcag tatgatgcaa atgcggcagt aattttaaat agttgccggt
    29461 atccaaatca atttgctgcg tacgcaaaat ctctgccgcg tcacataatt taaaatctgc
    29521 gtgcgttgcc aaaaactgct cggcaatgtt ttcattttca tcttgaagta aactgcaggt
    29581 ggaataaatc aaacggccac cggcttttac taatttggcg gcacgcgcca aaattgcagc
    29641 ttgcttttgc gtgagttcgg ccaaatcttc cggtgtttgg cgccacttaa ggtctggatt
    29701 acggcgtaat gtgcctaaac ctgtgcaagg cgcatccact aacacgcggt caaatttacc
    29761 attgagccgt ttgagttttt ggtcattttc actgctaatt ctttgcgccg ttaaattgct
    29821 taaacctgaa cgtttcaaac gttggcccaa gctgtgcagg cgcttttcag acacatcaaa
    29881 cgcatacaag cggccagtgt tgcgcataat tgcgcccatt gctagggttt taccgccagc
    29941 gccggcgcaa aagtcagcca ccatcatgcc gcgtttgggc gcgactaaaa acgccaaaat
    30001 ctggctacct tcgtcctgta cttcaatgtg gccttccgta aacaaggcat ggcggccaat
    30061 attcaaccgt aacggcatgc gtaacccggt ttgcgagtaa ggcgttttag taacattacc
    30121 ctcgcctgtt gcggcttctg ctgcgaattt agccattaca tcatcacgcg tggctttaat
    30181 ggtattcacg cgtaaatcaa gcgtggcaga ttcatgcatg ctacgcgcaa tagtaagcgc
    30241 ttccttttca ccatattgcg caacgagttt atcccacaac caatcgcgta cgtccgcttg
    30301 cacagctaac ggcaaatttt cagtggattt cgctttaatt gcttttgccc attccaactg
    30361 ttgctcgttg agcatgggtt ccattttttg gatgctcatg ccctgaatgc gtaacatcca
    30421 ggccaaaata agtttacgtg cgtcatctga gtcatgttca tcattggcag tcacagtgct
    30481 taaaaagcgc aggcggcgca aaacgccata cacactttca gccacaaagg cccgctcttt
    30541 ggtgcctaaa tcacgattat tgcgaaaaaa ttcacttagt tttgcatcag cagggctgtt
    30601 aaattcgagt atgttcgaaa gcattacggc cgcttgccgt attaaattgg gagtcatggg
    30661 gtttctctat attcttgatg ttgtttgcag cattttaatg tgctgcttat tcggttttta
    30721 atgctttcac cagcaacaca tacactttac cgtctttttc tgtttcacgt attttaacag
    30781 gtacgtactg ataatctaag gccaaccata attcagttgt tttttctttc tcagctgaag
    30841 atctgagcaa atgaacggtg tttaattcac ccattttagt tgaaatgact tcttcaccct
    30901 caaaactgta gtcatatacc cccacttttt tgccattggt aatatttaac tgcatgtttt
    30961 gtaagggcgg cacatacata aactgaaaca taaaactcag taaatcttgt gtgccatccg
    31021 ccagtacttt agcttggctc ccgttgacgc tatgcaaatt gagctgatgc gcttcccaat
    31081 caaaatcggc cgaaaatgtt ttattttgtt tgttaccaaa ctggtataaa taatgccttg
    31141 gttttaagcc cgcctgacca atatcaccag cacttgtttg caataaatct ggaatcacaa
    31201 gtgcggccag tccacgcgcc ttgattaaac ttttgatctg ataagtgttg tcattcggca
    31261 tccgctcaaa cgccatatgc gcacttcccg cagggctatt gcttggtgag gcttcacctg
    31321 ccgtgtacac atcaaattct gcatccacct tttggtaagc attcaaatta atttcgattg
    31381 gattttcttc agtctgcgca tcattcgcgg tttcaatctg ttctggttgt gcaatttgcg
    31441 ctgatgcttc aggtgcaact aattcagggc ttaacacctc tgtagcccct aaatcagtgg
    31501 cgggttcaat ttccattgac ggcgcttcaa ccgcagaatt tacatctggc ttagcatcaa
    31561 caggctttgg tttgggctca atggcttttt tttgcttatc aatactgggc gccggtggtg
    31621 gttgaggcgt ttctagtgct agttgaggcg ggggttgcgg tacaagtaag gtcgccacaa
    31681 tgacaagtgg tttttctgct agcgtgggta aattgaaata aaaccgacca atcagaaaaa
    31741 aatgtaacaa caacgataaa cctagtgcaa atacaaaccg tctaaagctt gcatcgctta
    31801 acaacccctt cagttgaata atgaacgcat tattccacat ggagtttcgt taaagtttgc
    31861 tctaacttcg caccatgctc atccaccatt aaaaagcgca aaagcaaata atgacggtct
    31921 gctgccaacc aaagctcttt ggtttcaact tggccttttt cattattttt aggcactaaa
    31981 tgaatggctt tataagattt tcccgcaaca tttagcgtgc ttggcatttc attattactg
    32041 tattcatagg tatttaattt cttacccgtg gtgagtttca caatgacgtc ttttgccaac
    32101 ggcttgggta agtacataaa ttggtaggca aagctggcta aatcttgcgt acctgcggtg
    32161 agcgttgcct cgcgcggctt gccttttact tgcatacgca gggtgttttt tggccaatca
    32221 aagtcggcaa ataaggcttt ttttggattt ttaccttggt gtagttcaaa atggcttggc
    32281 tttaagcctg tgcgcgtcac tttgccggta ctgactaatt tgcgaacccc cagtagggca
    32341 taaacgccta tgcctttggt ttcgctttca attttgtagg tattgccggt tactacaaac
    32401 gtttcacgca catcggcaaa cgccatgcca tctttggtta cgctgtattc agcttgtatg
    32461 cgctttggca aatctgccgc aagtgacgtg ccagaaaatg agaaaattaa gctcactaaa
    32521 ataaatgcgc gaatcaagtt aatcacaagc agtccttaaa atgtcgcgtt aaaaatttaa
    32581 acgccagcac ttaacgtatg aaatccattt tcggatacat ttacaccttc aattaaacca
    32641 ccaccgtagg tgcttcgcct tctgcctgcg cgcgaatttt accaatttca aacacggttt
    32701 cgcctgcggc ttctaatagc gccttggctt gtgcaacatg cgcgctactc aacacaatgg
    32761 ccataccaat gccgcagtta aaggttttat ataattcact tggcacaata ttgccttgcg
    32821 cctgtaacca ttgaaataat ggcggcaatg gccagctagc agctttaatt tcagcagtta
    32881 agcctgctgg aagtacacgt gggatatttt cagtaatgcc accccctgta atatgcgcca
    32941 tcccttttac gggcaaagta tcaatcaatt ttaaaattga tttcacataa atgcgcgttg
    33001 gcgccatgac aacatcttta aattttctac catgaaaatc tgactcaaaa tccacccctg
    33061 atttatcaat caatttacga atcagtgaat aaccatttga atgcgcacca ctggaagcca
    33121 aacccagcac cacatcaccc accgcgatag tcgtgccatc aatgatattg gctttatcca
    33181 cacagcctac tgcaaaacct gctaaatcat attctccagc agggtacatg cctggcattt
    33241 ctgcagtttc accccccacc aacgcacagc cagattgttc gcatccttcg gtaatgcctt
    33301 taatcacctg cgcggccgta cccacttcta acttaccgca ggcaaaataa tctaagaaaa
    33361 ataacggttc tgccccttgc accaaaatat cgttaacact catggcgact aaatcaatgc
    33421 ccaccgtgtc gtgtttattg agttcaaacg ccagtttaag cttagtgcct acgccatccg
    33481 tgccagacac caaaatcggc tctttgaatt ttttaggcat ggcaaaaagc gagccaaaac
    33541 caccaatgcc gcctaatact tcagggcgca tggtgcgttt cgcaaatggt ttaatttgct
    33601 caacgagcgc atcgcccgct tcaatatcca cgccagcatc acggtagcta atagaggtgt
    33661 tatctgaatt atttgcgctc aagagggttc tcgctttcta caaaagaggt gttctatcgt
    33721 tataatgcca ttttaactga aatgctggtt taaccgaaat tttagctagc caaatttatt
    33781 agcaaaaata atgtgagcaa aaaaatatgg aaaagcgcaa cccaatgatt tggtggctgg
    33841 ctggcttact cgtgtgtggc ctaatttatc tattaagccc aatacttacg ccttttttag
    33901 tcgcagctat tttggcctac attgccaacc cgctcgtgaa taaaattgat gcatttcaat
    33961 ttaaaaattt aagccccagc cgtacggtcg ccagcctgct ggtgatgtgc ttgatattgg
    34021 cggttatctt gctgatttta ttgattgtgg tgccattgat tcaaaaagaa gtcgtgctgc
    34081 ttttgcaaaa gctacccgct tatttcaaca cggctaaaag cagcttagag ccttggctac
    34141 aaaaaaactt tggtgttggt attgacattg attttgcaca aattcagcaa attctcaccg
    34201 agaattggaa aaccgcaggt aattttgcta aaaatatcgc gcaaacatta agcaccaaag
    34261 gcctagtctt tattggttgg ctggccaatt tggtgttgat tccgttggtg ctgttctatt
    34321 tattggtaga ttggaacatt ctcgtcaata aagttggcca attgattcct agaaaataca
    34381 tcgctaaaac gcaagaaatt gcccttgaaa ttgatgctgt gttggctgaa ttcttacgtg
    34441 ggcaactttc agttatgttg ctcatgagca tgttttattc tgctgggtta tggctggctg
    34501 ggcttgaagt tgccgtgcct atcggcattt tatctggctt attaggtttt atcccctact
    34561 taggctttgg gcttggttta attcttggcc tattctccgc cctacttcag tttggcaata
    34621 tcaccgatat tattcctgtg ttagtggtat ttggcctggg ccaaattgtt gagagcatgg
    34681 tgttaacccc ctatttagtg ggggaccgta tcggtctgca tccgctcgtt gtgatttttg
    34741 ctttaatggc tggtggccaa ctctttggct ttgctggtgt gctgctggcc ttacctgtat
    34801 ctgcagcgct tgcggttggc tttcggcatg ccaagcaaac ttatcttacc agccaatttt
    34861 ataatggata attgcgtttg aaacagttac ttttagacat tcagccactt gcaccaccaa
    34921 cgcttgaaaa ttttattatt ggcaaaaacg cagaagcact acacagcatc agcaatgcat
    34981 taaaccagca ggcagatacc cgatttatct acctttgggg catacaaggt agcggcaaaa
    35041 gccatctact ggaagcctgc aaacaaattg cagtgacaag aaatttgcct gttagcgtga
    35101 ttgatgatgt gcatacgctc gatgaaaatg cgcaaatcac cttgtttgac cactttaacc
    35161 agctacgtgc tgggaatggc attttgatga ctgccggcaa tgccgcgcca acgcaaatgg
    35221 gcttacgcga tgacctggca acacggcttg cgtggggctt agtgtatcaa ctgcacccgc
    35281 tcaccgatga agaaaaggcg caggcgctta aaacccatgc aaaagcacgg ggcatgaagt
    35341 tacccgatga agtggtagat tattgtttgc gttatttgcg ccgcgatttg cccacattga
    35401 tggccgtttt agatgcattg gatgaatggt cgctcacaga aaaaaaacct gtgacggtgc
    35461 cgatgttgaa aaaattatta caactaaact tagaaattta aacttaatcg ccatttaaat
    35521 tgatagatag acactatcca cgaggtcacc atgcaacacc atgttgagat tattttatgc
    35581 tacgcataac agaaatcaaa ctccctattg aattagcaga tacgctcatc catcaggatg
    35641 accaaatcaa ggcggcgtta ttaaaacgtc ttgaaattcc tgaaaatgaa ttgatcaact
    35701 tcagcatttt taaacggggt gtcgatgcgc gcaaatcgta tgcgattttg tatgtgtata
    35761 gcttagatgt tgaagtaaag cacgaggcaa aaattctcgc caagtttaaa aaagatgcgc
    35821 acattaaacc tgcgcctgat accagctatc attttgttgc taccgcgccc gttcgccctg
    35881 agctggtcgt tgggttaagt ggttcgacag gctcactacg aacggatttt aagagaccaa
    35941 tcattgttgg ctttgggcct gcgggtatct tcgctgcgct cattttagcg caatctggct
    36001 ttaagccaat tgtgttagag cgcggcaagg cggtgcgtga acgcacgcaa gacacgtggg
    36061 gcttatggcg taaacatcag cttaaccctg aatcgaacgt gcaatttggt gaaggtggtg
    36121 ccggcacatt ttctgacgga aaactgtata gccaaattaa agaccccaag cattacggcc
    36181 gcaaagtgat acaagaattc gttaaagccg gcgcacctga agaaatcatg tatgtgagcc
    36241 acccacatat cggcacattt aggctcgtgg gcatggtgga agaaatgcgt aaaaccatca
    36301 ccgaattagg cggtgagatt cgctttcaat caaaagtgga agatattgag attgcacaag
    36361 gcgaagtgca aggcgtggtg ctgcaaacgg gtgagcgcat tgcgacgcat catttaattt
    36421 tagccgttgg ccacagtgcc cgcgacacgt ttgagatggt gcatcgtaag ggcatttatg
    36481 tggaagccaa agctttctca atcggctttc gtattgagca cccacaatca ctgattgatc
    36541 aagcccgtta tggtaaaagc tacagcgatg acctgctgac caaactgggt gcagctgatt
    36601 ataaactggt gcatcacgct aaaaatggcc gttctgtcta cagtttctgc atgtgtccgg
    36661 gtggcacggt agtggcggcg gcttctgaac ccaactgcgt ggtgaccaac ggcatgagcc
    36721 aatattcacg caacgagcgc aacgccaacg cgggcattgt ggtgggtatt acgcccgaag
    36781 tcgattaccc cgcgcatcca ctggctggca tggaattaca acgtcaatta gaaagccatg
    36841 cgtttgtatt aggcggcagt aattacaatg cgccagccca gcgtattggc gactttttag
    36901 ccaatgtgcc ttcaacgaaa ttgggtgaag tcatcccctc ctacacgcca ggtgtgcaac
    36961 ttaccaactt agatagcgct ttacctgaat acgccattag cgctattcgc gaggcgattc
    37021 ctgaatttgc aaagcaagta aaaggctttg atttgcctga cggcatttta actggggttg
    37081 aaacacgcac ttcatcacct attcgcatca aacgggatga cgatacgcta caaagcatca
    37141 acaccaaagg tttgtacccc tgcggtgagg gcgcgggtta tgcgggcggc atattgtccg
    37201 caggcgtgga tggtattaaa gtggctgaag cggttgcttt aagtattgtg aataaaggat
    37261 aaatcatgct gtctcgcatc atctcgctgg ggttcttact gttactttct tacgctagct
    37321 ttgcagcggc ctcccccgcg cagcagatga tccatgcgca actcgaagcg caccccaata
    37381 aaagcggcgt ttatgtgtta gataaaggtg aagaagcatt actcgcgcgc gcctggctgg
    37441 cggaccacgc ggaaaaatcg attgaagtgc agtattttat ctggagtaca gacaatatcg
    37501 gcattctcgc gtctgaagcc ttattacgcg cagcaaatag aggcgtacaa gtgcgagtga
    37561 ttgtggacga cctactggta gatgcccctg ataaatcatt actcgcactc gccaagcatc
    37621 ctaatatcga tattcgtatc tataacccgc agcattctgt aggcacgcct tttcacaagc
    37681 gtttgctgaa tatggccata aattttcgcg gctttaacca acgcatgcac gacaaaactt
    37741 tcattgttga tggcaagctc gccatcaccg gtgggcgcaa catggcggcc gaatattttg
    37801 attacaatca acaatataac tttagagacc gcgatgcatt attgctgggc gcagtcgtgc
    37861 agaacatgga agaaagcttc aatcagtttt gggccagcct tttgagtgcg ccggttgaag
    37921 agctttatga tggcttaggc atcatacaaa agcatgtaac tgtgaaagat gctgaaatac
    37981 aaaaaattta tgcaaactta catgcctacg ctgaaaatcc tgaaaatttt tcacctgaaa
    38041 accgcaaagc cattttagaa acaccgcagg cgtttttaaa actcgctgag caaattgttt
    38101 ggacagatat tgagtttatc agcgacacgc cgggtaaaaa taaacaaaaa tttctactgg
    38161 gtggcggtgg tagaacaact gaaaaacttg cgcaactcgt tgaaaatgca gagcagcaag
    38221 ttgtgattca atcgccttac ttagtgatgt caaattccgc cataaaactt tttaaaaaag
    38281 cgattaaacg tggcgttaaa gtgcgcatta ataccaattc gctcgcctca actgataaca
    38341 ttcaggcgtt tagtggttat cgaaaccagc gcgatattct caccaaaatg ggcgttgaaa
    38401 tttttgaata caaaccagac ccgcaagtta agcagcatct tttacgccaa gcgatggcag
    38461 ccaatgagaa gccgcccgtt tttgccattc acgcaaaaac catggtggtg gacggtgaaa
    38521 ccgtttacat cggcaccttt aactttgatc cacgctcgca aaacctgaat actgaagtgg
    38581 gtgtaattgt gcgcgatacg gcacttgcaa gccaagtaca aaatgccatt gaaactgaca
    38641 tgcacccaga aaatagctgg aatgccgctg aagataaacc cgatcaattc gcttcttcag
    38701 ctaaacgaaa acgtgtggtt ttttaccagc taacgcccat caaaccattg ctctaatttg
    38761 cttaaaattt aagcaaattt aaggctattt ttcacacaac ctttttcgat gctctggaag
    38821 ccttgtaaat aaaggctcgc agggcattat ttttttctcg atgaatttct gtgccttgta
    38881 actcacgttt tttacatgaa ataaaccgtg aactattgca cttaaacgcg ctcgaagcgc
    38941 tatactcttc tttgcaactg tgattggatt tttcgcgttt tgtaaccttg caattctttt
    39001 cttttaaagg agaaaatcat gaaagcaaat gtaggcggaa ttgataaaat tttacggatt
    39061 gtcattggtg cggcgctcat tgcttgggtg ctgtttgcta acggcccggt gtgggcttgg
    39121 attggggttg tgccgctggc aacgggtttg tttaattttt gtgcgcttta ttctatcttg
    39181 ggcatcaata cctgcaaggt taaataactg ctcgccggac aagcacaggc gttaaatttt
    39241 ttttaccgcc tgtgcttttt acttcactct tctaacttct aatgatgtaa tcaaatgcgc
    39301 caagcgctgc ctttgcgccc tcgcccatgg caataatgat ttgcttgtaa ggcgtggttg
    39361 tcacgtcgcc cgccgcaaat acgcccggca atgaggtagc gttatgctgg ttgatttcca
    39421 cttcattaaa tttactgagt tttaccgcac ccttgagcca gtcagtattc ggcaccaagc
    39481 caatttgtac aaacacgcca tctaacgcta agtgttttga ttcgcccgtg acgcggtcgg
    39541 tataagtcag gccatgcatt ttgtggccat caccagtaat ttcagtggtt tgtgcgctcg
    39601 tcacaatggt gacattattg agcgtgcgga gttttttctg caaaacggca tctgctttta
    39661 attcggtatc aaattgcaaa agcgtcacgt ggccgacaaa attagccaaa tcgatggcgg
    39721 cctcaacccc ggagttgcca ccgcccacca ccgcaaccgg cttaccttta aatagtggcc
    39781 catcacaatg cggacagtaa gccacgcctt taccacggta ttctttttcg ccaggcacat
    39841 tgagttcgcg ccagcgtgca cccgtggcta gaatcaccgt tttgcttttt agcgttgcgc
    39901 cactttccag ctttagttca attaaatcat tatttttgtt gccttcttta cctggctcgg
    39961 taatcgcgac cacacgctgt agattcataa tctcgacatc ataacttttc acgtgttctt
    40021 ctagtgccat caccaacttt ggtccgtcag tctctttcac tgaaataaag ttctcaattg
    40081 ccagtgtatc catcacttgc ccgccaaagc gctcggccac aatgccagtg cgaaagccct
    40141 tacgtgccgc ataaattgaa gccgacgccc ctgcagggcc accgccaacc accaacacat
    40201 catatggtgc taaggcatcc agttttttcg cttcacgtgc aacggcacca ctatcgattt
    40261 ttagcagaat ttcatcgagt tccatacgcc cggcgccgaa tatcgcaccg tttaaatacg
    40321 ttgtcggcac agacataatt tctttggccg ccacttcatc ttggtacagc gcaccatcaa
    40381 tcatcacgtg ggtgatattg gggttaatca cggccatcat attcattgcc tgcaccacat
    40441 ccgggcagtt atggcagctt aatgaaatat aagtctcaaa atggaaagta ccctcaatcg
    40501 cggcaatttg tgcgattttt tcagcttcca gtttcatggg gtggccacct gtatgcaaca
    40561 gtgccagcac taaagaggta aattcatggc ccatcggaat acccgcaaaa cgcacgccaa
    40621 cggccctttg cgcttgacta tgccccgcac cacgcttgac tgcaaatgaa ggtttgcgcg
    40681 cattgtcctc catcaccagg gtaatcttgt cagacatacc agcgatttct tctaacagcg
    40741 catgcatctc tttggctgcc ggtgtatcgt ctaatgaggc tgccagtacg ataggctcgc
    40801 gcagcattgc taaataggtt tgcaattggg ttttaatgcc agtttctaat gccatgattg
    40861 ctctctttac tcattttgta ttgttgtgtg gcttaaattt aaccgcactt tattgaaaat
    40921 aaaaggcatt atcttttgaa taatgccttt gttatttaac agcgcgacaa gatgcttgct
    40981 cggtcgcgcc aattgacttt gcctaaggca aaaataaaac tttagaacct taaattttgc
    41041 ccactaaatc aagtgaaggt gttaatgttt tagcaccttc ttgccatttt gctgggcaca
    41101 cttggcctgg gtgactagca gtaaattgcg ccgcttttaa tttgcgcaat gtttctttta
    41161 catcacgtgc aatttcgttg ctatgaattt caactgtttt aatcacacct tctgggttaa
    41221 tcacaaatgt gccgcgcaac gccaggcctt cttcatcaat atgcacgtca aatgcacggg
    41281 tgagttgatg tgttgggtca cctaccaatg gaaattttgc tttacctaca gcaggtgaag
    41341 tttcgtgcca aactttatgt gaaaagtgcg tgtcagttgt gacaatgtag acttcagcgc
    41401 ccgctttttg aaaatcagca taatgatcag ccgcatcttc tacttcagtt gggcaattaa
    41461 aggtaaatgc tgctggcata aaaataatca ctgaccattt gccttttagg ctggcatccg
    41521 ttacttcaat aaatttaccg ttatgaaaag cctgcgctct aaatggttga acttctgtat
    41581 taattaatga cattgtgtgt ttccttttat ataaaaagtt gtgttaaaaa tagtaaaacg
    41641 actgcctaaa aactaatcac ttaattgatg ttgcacattc taaaccctaa ataaaaataa
    41701 atctaatata tcattttaat tgaattgatt aatttaattt atcatacgcc aacttaaatg
    41761 ccactctata tcatgtccgt tcggctaatc tttactttag ccttttttat cttccccctg
    41821 catacgctgt aactctttta ggcgtgcctc tacaatagaa tgctgataaa aatcaccatc
    41881 tttggctttt accgcaagct ccatttgctc gatggcgcgc tttaagtcat attttctgaa
    41941 ataagcttca ccctgcgctt ggtggcttaa taacatttta ttctgaatgg tgtaagcctt
    42001 ggcaagcact tcataaaact gtgcatcttt tgggtacagt ctttgttttt cttttacgag
    42061 ctgaatggca ttatccgctt gcttgagtgc caaaaagtga tccgcgtaac catacaacag
    42121 cgcacgatta tctgggtata ttttcaacgc ggctgcatat tgcaggcccg cttgttgcgc
    42181 attatttttt gccacttgca cgcgcgcgct taagttttca atcatggcat tgggtggtga
    42241 atttttttta agccaggcca cttctttttc ggcctgcgtg agtgcgtttt tacgtagata
    42301 agcaaccgcc aaaccataat gctctactgc ctcgttggcg gtgcggcgtt cacgaatatt
    42361 ttgctcaaaa accgcaatat tagtttctac cgcgccattt tgtgcttgca gctttgcttt
    42421 tacatactga aactccgcac tatctggcac ttgccgataa ggcatttgct caacgcgatt
    42481 cgccacatcc gcaatgcgct cacccgtcag cgggtgggta cgtaaaaggc tgggcgcacc
    42541 gccttctgaa aagcgtgagc catgctgcaa tgttttaaaa aatgctggca tgccgcgcac
    42601 atcaaagcct gcattgctga tgatttgcaa accaacgcga tcggcctcgc gctcgtgttc
    42661 gcgagtgtaa tctaattgat tttgcacgcc tactgccgag gccgttgtca tggcgccgct
    42721 ggctaattcc gggttggcac gtgccaccag caaggccaaa gcaaaaccgg caatattttt
    42781 aacggtatcg tatttttgtg cggctaccat acgggccaaa tggcgttgcg tcacatgacc
    42841 aatttcatgg cccaacacgc tggccagttc agattcacta ttggccgcca aaaataaacc
    42901 tgtatgcacg ccaatgacgc cgcccggcat ggcaaacgca ttgatggagg tgtcattaac
    42961 cacaaaaaaa ttaaatttct gctgtttatc agtactggca gcggccagct tattaccaag
    43021 tgcattgaga taatcaatca cttccacgtc ttgcacaacc tcatcgctca ccgaaacctc
    43081 gcgcatgatt tgctcgccaa tgcgctgctc atctaatggt gtgaaaacag tttgcgaaac
    43141 atcgcctaaa tcaggcaggc tggtgtcaaa attggtcgat tttgaatagg gattacctga
    43201 caaagatgat tcagccgcca cttgcaggca attagtggca agtaatgtgg caattacaat
    43261 agtttttaat ttcattgttg ctatgataat aacttctgac taaaagttgc actgattgag
    43321 actattatat gcccgattca aagttaaacc caccgcagca aacactcacg cattttgacg
    43381 ccagcggtca ggcgcacatg gttgacgtgg gcgacaaggc agtaacgcat cgcgtcgcca
    43441 cggccagcgg cacgattcac atgcagcccg ccaccttgca aaccattcaa caaggtgacg
    43501 ccaaaaaagg tgatgtgctt ggcatcgcgc gcattgctgc tattcaaggc gccaaacgca
    43561 cgtcagattt aattccgcta tgccacccaa ttagcctaac aaaagtagcg gttactttta
    43621 acatcgatgc agaaaacgct agcgttacct gcactgccac ttgtcacacc acaggccaaa
    43681 caggtgtaga aatggaagca ctgactgcgg tgagtgtggg cctgctgacc atttatgata
    43741 tgtgtaaagc catagaccgc ggcatgacca tgcgcgaaat taaactgctc gaaaaacaag
    43801 gcggaaaatc tggtcattgg actgcgtcat aactttaact ttattattta tcaaaatttc
    43861 aggaaaacag catgcttaaa ctaatattca tctgcacact ttcaatggta acgattacgg
    43921 ggtgcgagca acaaaaaaat gcctctgctg aagtaggcac cgtgcctaaa caaattatcg
    43981 acaaagccac acaagatatt aacaacgccc aggcgttggc aacagaaaac ttgaaagcgc
    44041 tagagcaatt agacgcgcca acggaagcgc aacagtaagc caagaacaag cttaaacaac
    44101 gcgaacgcct ctaaatttac ataaattttc attgcgaaaa catataaatg gttataatca
    44161 accaataacc atttttaaat taatattata gggctatgaa aaaacatgtt taatttttta
    44221 tcttctctca acattttcac cctcatttta ctcatcttga ttgtcatttt ttttggtttt
    44281 attctgtggc tgaatatgca ttcgaaggat taccgacaat ttcttgatga acagaaaaaa
    44341 cagcgcgaca attaactatt tcgcttgcat aaattccatt gcaaaaacat ccatttttta
    44401 atgaaattat atacaaccct tttacactgc cctgcaagcc ttataaataa aggctcacag
    44461 ggcagcgttt tttgctcgta gatttttcag ccgcaaaaat tggaattatt agagcaaatc
    44521 agccagtatt tttgcactgg ctggggccat tgtcacgccg tacctgaaat gcccagtatt
    44581 gagataaaga ttgtgaatgg tggggtgtct tgcaatggtc ggcagatttt ctggggcacc
    44641 tggccgtagg ccgctccaat gctgatgaat cggcatgcct tttaatgcag gcaagattgc
    44701 ttccgcttta gcctgcattt cacgccttgc ggtttctgtc acgtttgcgt caaaacccac
    44761 atcttctagc gtactgcctg ccagcaaaaa gccatcgcga cgttgtagca tataaaagcc
    44821 gtcctggtag agaatgtgct gcaaggtgtt aactggcatt tgatacagca atatttgtcc
    44881 gcgcattggt ttaatgtcaa tatttgccgc agtttctttt agcagttcaa agctccatgc
    44941 gcctgaagtg acgatatata aatcggcccg taacgtttca ccttttgtgg tttgccaacc
    45001 atttaaactg gccgtttctt gaatgggcgc gagttgcgtg tgctcgatta gcgtcacctc
    45061 atgcaatatc aaccactgtt ttaatgcttg cagcaagcgc ggattacgca cttgtgcgac
    45121 attgggcagc agtaagtcat caccaacatg cgtgaactca atcttatttt tttcacacca
    45181 ttgttgggct aaggctaaat cataaggcgg caaaattcgc atgccacact tggtgagttc
    45241 tgggtctatg ccagtttcac gcaaaagctg ctgcgttacc tgcgggtaaa gattggcacc
    45301 agcaagcgca agttggttga cttcatcacg gtacatccaa ggcaataatg gaaaaacaat
    45361 gcccgcacct gcccatgaag actcacctga agtctgtgca gcaatttggt tgcgttcaac
    45421 aatcgtcacc cgataaccgc gggtgacgag ttccattgcc gtcatacagc cagcaatgcc
    45481 gccgcccaca atcatcacat gcgtcatttt gttgcaacct taccaattaa catcgcattt
    45541 tgcgcattag ttaaaccaat cggctgacca agcagcacga ccacatcacc ttcagcaagt
    45601 tggctttcgg gcgtgggtac tgtgccattg gagtttggcc ggcgaattga tttcacttca
    45661 acctcaaaat tctctaattg catatcggca agccgcctgc caatggcgta gcttccaggt
    45721 gaaataatca cagagtgcag gcgaacctgc tgcttttcca aggcatcttc ttcaacatcc
    45781 gaaataccat gaaaataacc tttaaataac ttatagcgct cttcacgaaa taggcgaatc
    45841 cgctttacta ctttatttaa tggcacaccc atcaacatta aggcatgtga agccagcatt
    45901 aggcttcctt caagaatctc cggtaccact tctgccgcac ctgcggcctg caaggcttcc
    45961 atatttgagt catctacagt gcgcacaatc acaggtaagt gcggatagtt ttcttgcaca
    46021 atatgcaaaa ttttcatggc ggcgcgattg tcagcataac tgatgactaa ggctttggcg
    46081 cgcgcactgc cggccgcttc taacactgcg cgccttgcgg catcgccaaa cattacattt
    46141 tcacccgctg cggcggcctc taaaacacgc gtagggtcaa tatccagcgc cacatatgga
    46201 atatgctctt ctattaaaaa gcgcgctaaa tactggccac ttcggccgta accacaaata
    46261 accacatggt tatgcatgtg tttgccaaca tcatcaatat cttgcaccac ttggccacta
    46321 ttgcgcgtgt aactgcgcgc cattttgcgt gcaatacgtc cgttgtactg aattaacaat
    46381 ggtgcaatca ccatagataa taatgaagcc gccaaaatga cttgtaatgc ctcgccacca
    46441 atcagttttt gttccacgcc taaggctaaa atcacaaaac cgaattcacc cgcttgcgcc
    46501 aaaataaggc ctgttctaat cgccacgcct atttcgtatt ttacaaatcg taccaccagt
    46561 gtcacgatga ccgcctttag cagcacaaaa gacacgagca ccaacaaaac caagccaatg
    46621 tgctgaagta tttgcgccat gttcagtagc atgccaatac taatgaaaaa caacccaagg
    46681 agaatatctc tgaacggcga aatatcagac tcaacctgat agcgaaactt agtctctgaa
    46741 atcagcatcc cggcaataaa agcccccagc gcataggaaa gcccggctaa tttggtgaca
    46801 taagccaata gcaacgtaac catcagcaca ttcatcacaa ataactcgcg cgagcgttgg
    46861 ctggcaacca agttaaacca agggttaatt agccacttgc caaccgtaaa tagaaacaac
    46921 aacatggccg cagctttgag cattgccatg ccaagcacat ctaaaagctt ggcaccttca
    46981 gcgcccaaag ctggaatcag caccagtacc ggcactacgg ctaagtcttg aaaaagcagc
    47041 acgccgatac ttaatcggcc atggcgcgag ttgagatcaa cccgctcagc gagtattttt
    47101 gaaacaatgg ccgtagaaga catagccaat gccgcaccca ccacaaaggc ggctgaccaa
    47161 tccaaacccg ctaatttagc aatgcccatg acaatcgcca aggtcaatac aacttgcgca
    47221 ccccctaacc ccagcacttt tttacgcatg gcgtatagtt gcggcaggct aaattcaagc
    47281 ccgatgctaa acattaaaaa tacaatgcca aattctgcaa gctcgtgtgt tgcctcagta
    47341 tcaggcaata gaccaaaagt atgtggacct aatactgcgc ctacaaaaaa ataagccagc
    47401 atggcaggca ggcgaaattc acgaaatagc gctaccgcta gcaccgcgct tactaataac
    47461 aataaaattt ggggtaaggt gtcaaacatg ggttgtcatt taatctatct ttggcgcggc
    47521 cattttaatg gtctctattg tttcataact agaagcgaaa cacagcaaaa aaatgccgat
    47581 agcaaaattt gtgcctaatt ctaatatttt tgaatgattt tactatattt agcacttttt
    47641 acgctgatta aggctttata tctcgcgctt tcccttttat actaatgtgt agttatgtca
    47701 atttaaagaa atcattttta gtaatgccct cacttcctaa gacgcaaacg caagtaaccg
    47761 ccaagcagcc aactttatcg ttggcgcgtg atgtattgct actggaagcc agtgaaatta
    47821 acgcgctggc caataggctc gaccagcatt tttctgatgc agttgcgctt attttgcaat
    47881 gcaagggccg cgtggtggtc agcggcatgg gaaaatcagg ccatattggt ggaaaaattg
    47941 catccacctt ggcaagcacc ggcacccctg cattttttat gcatcctgcc gaggcaagtc
    48001 atggtgattt gggtatgatt accgctggtg acatcgttat cgcactttca aactctggtg
    48061 aaagcgatga gctgctcgcc atcattactc cgcttaagcg gctgggggcg agcattattg
    48121 ccatcaccgg gaataatgcc tcatctttag cactggcggc tgacattcat ctaagtgcga
    48181 gcgtgagcaa agaagcctgc ccattaggac ttgcaccaac ttctagtagt a
//
LOCUS       DAOQSI010000002        47365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_362_length_47365_cov_6.301522, whole genome shotgun sequence.
ACCESSION   DAOQSI010000002 DAOQSI010000000
VERSION     DAOQSI010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 47365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47365
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_362_length_47365_cov_6.301522"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /gene="sucC"
                     /locus_tag="PKL42_00220"
     CDS             <1..622
                     /gene="sucC"
                     /locus_tag="PKL42_00220"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985876.1"
                     /note="catalyzes the interconversion of succinyl-CoA and
                     succinate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HNU65518.1"
                     /translation="YRLFKENDLALLEINPLVVTTDGKLFALDCKMSIDDNALYRQKA
                     LAEQRDWSQEDSKEAIAHHAGLNYIALNGNIGCMVNGAGLAMATMDLIKLHGGAPANF
                     LDVGGGATAETVTKAFKIILEDHNVKAILVNIFGGIMRCDIIAEGIITAVKEVGMTIP
                     VVVRLEGTNVELGKQMLRESGLNIISAENLTDAAKQAVNSVKGQAV"
     gene            619..1506
                     /gene="sucD"
                     /locus_tag="PKL42_00225"
     CDS             619..1506
                     /gene="sucD"
                     /locus_tag="PKL42_00225"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNU65519.1"
                     /translation="MSILVDKNTKVLCQGFTGKQGTFHSEQALAYGTKMVGGVTPGKG
                     GQLHLGLPVFNTMRDAVRDTQANASVIYVPPAFAADSILEASDAGIELIICITEGIPV
                     LDMLNVKAALKANGTKLIGPNCPGIITPDECKIGIMPGSIHLRGKVGVVSRSGTLTYE
                     AVHQTTALGLGQTTCVGIGGDPIKGLNFIECLTMFEADPETEAIIMVGEIGGSDEEAA
                     AEFIKSHVTKPVAAYIAGQTAPAGKRMGHAGAIISGGSGKAVDKIRALERAGAVVASS
                     PAGLGEAVLDAIKANKKVA"
     gene            1514..2458
                     /locus_tag="PKL42_00230"
     CDS             1514..2458
                     /locus_tag="PKL42_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNU65520.1"
                     /translation="MKTFLKRFAFILLLTTMPVMAAEDIAYLLTASSKGDLATVNALL
                     ASGANPNAKDGEGITALMYAARKDKAEVAASLIEKGADMNAVDNGGWTPLMYAAKKNH
                     IATIAVLLEKGADPKIRDTAGWSALGLAASSGFSQSVDLLIKHGIDVNAKSDSGKTVL
                     MYAAKNGDIPTIDILLANKADPRAQDKLGATALMIAAREGHAPVVTLLAKAGTNVNQK
                     DRAKWTALTWAVKKSNIEAAAVLVEAGADVNNQDAEGTPLLHVAVDSGKAEIVKLLIE
                     HDVNVKAKDQYGLTALVYALKGQHTEIAKMIKAAGGKY"
     gene            2472..4088
                     /locus_tag="PKL42_00235"
     CDS             2472..4088
                     /locus_tag="PKL42_00235"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNU65521.1"
                     /translation="MKIAIAQMNCIVGDIAGNVAQIIAYSQQAKAQGASLVVTPELSL
                     CGYPPEDLLLRDDFLQACARGLKQLCEETQGLTLIVGHPHQVGEFCYNAASVIQHGKL
                     LATYHKHSLPNYSVFDEKRYFAAGEHALVFEHQDVNIGVLICADVWEPAPALKAKAAG
                     AQLLIALNASPFHIEKHRTRLEVLRERVAETNLPIMYINMVGGQDELVFDGGSFVLSD
                     TGAITHHLNAFEQALAIVDCQAAMPLTGKVAPHLSLEASVYQALTLGLHDYVRKNGFP
                     GVVLGLSGGIDSALTLAIAVDALGAEQVHAVLMSSEFTANMSVDDAVEMANILGVKHS
                     LIPIVTLFEHFRSALSTEFAGLPFDTTEENLQARIRGMLLMALSNKFGSIVVTTGNKS
                     EMAVGYCTLYGDMAGGFALLKDVPKTLVYKLSQYRNSLSQVIPQRVITRPPSAELREN
                     QLDQDSLPPYEILDGIIEAYVEDDLSRADIIAQGYQAADVSRVMKLIDRNEYKRRQAP
                     VGVRITDRGFGKDRRYPITVKLDFDTLFTE"
     gene            4154..4492
                     /locus_tag="PKL42_00240"
     CDS             4154..4492
                     /locus_tag="PKL42_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNU65522.1"
                     /translation="MKKIEAIIKPFKLDEVREALSQIGVNGLTATEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKIKLELVLPENLVDSAIEAIIKAAHTGKIGDGKIFVSAVEQVIRI
                     RTGETGESAV"
     gene            complement(4603..5031)
                     /locus_tag="PKL42_00245"
     CDS             complement(4603..5031)
                     /locus_tag="PKL42_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNU65523.1"
                     /translation="MSQLARFNNALLNDFFGDRRTQGYFVSPLHGEGVASSFKVDIKD
                     TDKAYVFHAEIPGVKKEDLNLTVDGSTVTIAAEIKQHDQKTEDGQVVRSERYFGAVSR
                     SFQLPVDVDQEHAEASYENGVLELTLPKKITIAGKRISVR"
     gene            complement(5230..6477)
                     /locus_tag="PKL42_00250"
     CDS             complement(5230..6477)
                     /locus_tag="PKL42_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HNU65524.1"
                     /translation="MNKKTSNTVAIIGAGPAGLMAAETLIAQSVDVHVYDAMPSVGRK
                     FLMAGIGGMNITHAEPAAQFITRYGDRQNQIAPMLEQLNAASLRDWVHQLGFETFVGS
                     SGRVFPTDMKAAPLLRAWLHRLRHAGVHFHVRHQWQGWDAQQNLCFHTPQGAYFTKPD
                     AVILALGGGSWAKLGSDGKWLPLLAQQGIKVATLKPANCGFNVSWSDYFQARFAGAPL
                     KTISVNYADKHDAIHEKSGEIMVTSNGVEGSLIYAISATLRDEIEANGHAQIHLDLLP
                     QKNIDAVIQAIAHPRGARSLSSHLQSRLNLKGVKVALLREVLTAEAMNDPVQLGNAIK
                     ALPIVLNSTRPLDEAISSAGGVTFTDLNEELMLTKLPGVFCAGEMLDWEAPTGGYLLT
                     ACFASGRVAGNGAWSWLQKNQLN"
     gene            complement(6603..8393)
                     /gene="feoB"
                     /locus_tag="PKL42_00255"
     CDS             complement(6603..8393)
                     /gene="feoB"
                     /locus_tag="PKL42_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNU65525.1"
                     /translation="MKRIALLGMPNTGKSTLFNRISGASARVGNWPGITVDLMSAKIL
                     MGGHIAELIDLPGIYDLHGFSEDEQVVRHFLANNAVDLVIILLNSSQIDRQLSLVLQI
                     QKLNLPAVLALNMADEAKRAGITIDTESLTRELNMPVISISAKFGDGLPKALQAATTI
                     INQNKTATDPQQISQLLQEDNQIEREMEQLIAHHVQIPATLSDNTTDKIDRILLHKWL
                     GLPIFFAAIFLLFQFIFTAGKPLQDGLAWALDLLRTAALEPLFANSPAWLSGLMLDGI
                     FNGVATVAAFVPIIILFFLVMSMVEDSGYLSRAAFLMDALMAKMGLDGRGFVMMLMGF
                     GCNVPALMGTRIMRSRPMRLLTMLVIPLSLCSARLQVFIFMITALFTAKQAPFVLFTL
                     YIMSFATMFLTAVLFQGQYKNTEPFILELPPYRFPTLRQIVLRGWHEVKHFLNRATKF
                     IIIGVVLVWVLTNFPSDVPPASSGTYAGQIGTLFAPILDPIGINSQLAIALIFGFVAK
                     EIVVGSLAVIYGMQGDALMVQMATQVDWVQAMSFMLFTLIYTPCLSTVATLRSESKSS
                     AFMWLSIVWSLSLAWVVSFVFYQGARALGF"
     gene            complement(8395..8622)
                     /locus_tag="PKL42_00260"
     CDS             complement(8395..8622)
                     /locus_tag="PKL42_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HNU65526.1"
                     /translation="MQLLSQLNIGAPAVISAIEADESLFHRLSALGFRVGKPLSIIRR
                     ANFKGPLQVRLGTTDVILRAAEAARIRINTL"
     gene            complement(9060..9848)
                     /locus_tag="PKL42_00265"
     CDS             complement(9060..9848)
                     /locus_tag="PKL42_00265"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNU65527.1"
                     /translation="MKFTQTLIAAAIITLAPAAFAAETIATVNGKGINKSVYDYIAKD
                     ASARGQKIDDQVKAAITNKLVDSEIVYQEAVKLGLDKQSDYIAREELSRRELLTSAYL
                     QDFVKKNPISEADTKAAYEEYKKAYGDKEYSARHILVKTEAEAKEIIAKLQKGGDFAK
                     TAKEKSLDPGSKDKGGDLGWFSPATMVKPFSDAVAALPKGKTTAEPVQTQFGWHVIKS
                     IDSRAAQPLAYDKVKDGLQKNLQQRNLEKMMADLRSKAKVDIAK"
     gene            complement(9902..10162)
                     /locus_tag="PKL42_00270"
     CDS             complement(9902..10162)
                     /locus_tag="PKL42_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HNU65528.1"
                     /translation="MNLITEITHRLQSLEPTQLEIMDESAQHQGHARNTGGGHFKLKI
                     TSSHFSQKSQIMRHRLIYQLVADLIPNKIHALSIQALAPSEA"
     gene            complement(10190..10489)
                     /locus_tag="PKL42_00275"
     CDS             complement(10190..10489)
                     /locus_tag="PKL42_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNU65529.1"
                     /translation="MWYAIIAEDTPNSLEKRLSARPAHLARLTALQEAGRLLLAGPFP
                     AIDSTDPGPAGFSGSLIVAEFSSLTAANTWANDDPFVQAGVYANVIVKPFRKTLP"
     gene            complement(10489..11115)
                     /locus_tag="PKL42_00280"
     CDS             complement(10489..11115)
                     /locus_tag="PKL42_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HNU65530.1"
                     /translation="MKKFLFDLFPVLMFFITLKVAEKTASASVWLAEIFSAVGLAVTV
                     KPDLVPVMLATVAVIIATVIQVAWVKFKHGKVDNMLLISAALIVLLGGMTLYFQNDGF
                     IKWKPTVLYWLFSVVLIAADLFWHKNLIKAMMGHAMELPELVWKKLNYSWAVFFILLG
                     VLNLYVAFNYSIDTWASFKLFGATGIMFVFIILQTLMLNKYLPKEDTK"
     gene            11292..12146
                     /locus_tag="PKL42_00285"
     CDS             11292..12146
                     /locus_tag="PKL42_00285"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-nucleoside bisphosphate phosphatase"
                     /protein_id="HNU65531.1"
                     /translation="MSLKIDLHCHSTISDGMLAPEDLVAYAARQGVNVLALTDHDDVG
                     GLKVARQAADEHGITLINGVEISVTWKKRTLHIVGLKINPDNETLKNALNQVHIDRDA
                     RAREMAESLAKAGIPEAYEGAKAIAKQSIITRVHFARFLAEKGYAKDTKSVFKKYLVK
                     GKPGYVNHQWMTLEQAVKLILDSGGIPVIAHPGRYDLGIINMLLLMHEFRSYGGVAIE
                     VVTSSHTPPQYQQFAKLAHQFGLKSSQGSDYHGQGISFMEMGYLPPLPANCIPVWQDW
                     PEVQEVTA"
     gene            12150..12785
                     /locus_tag="PKL42_00290"
     CDS             12150..12785
                     /locus_tag="PKL42_00290"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNU65532.1"
                     /translation="MSQLFNIHLENPQPRLITHTAEILRDGGVIAYPTDSGYALGCML
                     GFGDAQARIRQIRGVDDKHLFTLVCRNLSELGNYAIVSNSQFRLLKANTPGAYTFILN
                     ATKEVPRKLQHPKRSAVGIRVPQHPIVQAILEAIDAPLLSMTLHIAGDDEVLSEAWKI
                     RERLERLVDLVIDAGHCTAGATSVIDLTAEAPVLIRAGQGDLAPFGLDAVD"
     gene            12846..13490
                     /locus_tag="PKL42_00295"
     CDS             12846..13490
                     /locus_tag="PKL42_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNU65533.1"
                     /translation="MDLSIIQKIIIYALPIIFAITVHEAAHGYAARHFGDMTAFNAGR
                     ITLNPIKHIDPFGTILLPALTVMLGGILFGWAKPVPVNFGALRNPKKDMLWVAAAGPA
                     SNFVMAVFWAIMFKLAIGAPEAIAVPLALMAKAGVSINIVLMVLNLLPLPPLDGGRIA
                     VSLLPHHLARPFAQIERYGFFILIALLFTGVLGMVLGPLVNFASNFIYSFVNLL"
     gene            13524..14726
                     /locus_tag="PKL42_00300"
     CDS             13524..14726
                     /locus_tag="PKL42_00300"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNU65534.1"
                     /translation="MAIERVLSGMRPTGNLHLGHYNGVLKNWIRLQHEHECLFFVADW
                     HALTTHYDTPEIIEESVWEMVIDWLAAGVDPANATIFIQSRVPEHAELHTLLSMITPL
                     SWLERVPTYKDQQEKLSNKDLSTYGFLGYPLLQSADILIYKATQVPVGEDQIPHIEFT
                     REIARRFNHIYGKEKGFEEKAEAAIKKLGSKRAALYEEMRNAYQQSGDEEALESARAM
                     IEEQQGMSMGDKERLLGYLEGGGKIILVEPEYKLTPASKMPGLDGQKMSKSYNNTISL
                     RENPETIAKKIKTMPTDPARIRRTDPGDPAKCPVWQLHEIYSEDNVKDWVQMGCKGAT
                     IGCIECKGPVIEGVLKELKPIQERAAQYAEDPTLVKNIVAEGCEKARKLARETMRDVR
                     DAMGLSYS"
     gene            14735..15520
                     /locus_tag="PKL42_00305"
     CDS             14735..15520
                     /locus_tag="PKL42_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HNU65535.1"
                     /translation="MLLETSINAKIKGELLTELPQDLYIPPDALEVYLESFEGPLDLL
                     LYLIRKHNLDILDIPMADLTRQYMVYVEKMKAIKLELAGDYLLMSAMLIEIKSRMLLP
                     KPAEIAEEDDPRAELVRRLMEYEAIKLAAQGLDEMPKVGEELSVAAAYYQHISEIKPP
                     EVSLDELAEAWRNVLKRAKLNSHHKIGRAELSVREHMSQILRRLKTDGLVEFSQLFDI
                     VNDGLPKLVVNFLAILELAKEGLIRITQQEAFSPIYLQVNGAD"
     gene            15669..16220
                     /gene="scpB"
                     /locus_tag="PKL42_00310"
     CDS             15669..16220
                     /gene="scpB"
                     /locus_tag="PKL42_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNU65536.1"
                     /translation="MKEPENIIEMKQVLEAALLTSAQPLSLDDMLKLFAGRMERATLR
                     MLLDELKVDWEPRTMELVQVATGYRFQAKAEYSEFIARLNPEKQPKYSRAVMETLAII
                     AYRQPVTRGDIEEIRGVAVNPNVMRQLQERDWIDVVGHREVPGRPSLYATTKHFLDDL
                     NLRSLAELPPMEVFAQQEMPLDS"
     gene            16364..17488
                     /locus_tag="PKL42_00315"
     CDS             16364..17488
                     /locus_tag="PKL42_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNU65537.1"
                     /translation="MARPFKTQRDPQATPRRPKTDFKKIEIDENAPPAPTQRLHKLLA
                     LAGLGSRRDMELLIESGRVTVNGKPAVVGQGVTEHDVVRLDSRPLKLPFEAELPQVLI
                     YHKPEGEIVSQDDPEGRASVFDKLPKVKHGKWIAIGRLDMNTSGLLIFTTSGELANHF
                     MHPRYEVEREYAVRIFGELTEGQMTQLTEGIELEDGPASFDSIRPQGGEGANHWYQVI
                     LREGRNREVRRLFEAFQLPVSRLMRVRFGPVNLPPRVKRGTMLKLEQKEVVGLLEWAG
                     LPVPNAPLRQLTQREKDKATSVFVPKVRKQRVSALDRPPREVGERTSYRGNNAPNSAT
                     KKPALNSNKPKRPANRRVRQGSDLNAPTMQKKSDRNRGRG"
     gene            complement(17643..18452)
                     /gene="dapB"
                     /locus_tag="PKL42_00320"
     CDS             complement(17643..18452)
                     /gene="dapB"
                     /locus_tag="PKL42_00320"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNU65538.1"
                     /translation="MANPLKVVIAGCTGRMGHVLLESVFADADLKLHGALDRAGNPQL
                     GRDAGEQFGKNSAVKITDNMDEALKNADVLVDFTRPEASMQYLAACEKAKVKLVIGTT
                     GFSAEEKATIAKAAKNISIVFAPNMSVGVTLLINLVEQAAKVLANGYDIEVVEMHHRH
                     KVDAPSGTALRLGEAAAHGIGKALSECAVYAREGVTGEREAGTIGFATLRGGDVVGDH
                     TVVLAGIGERVELTHKASSRATFALGALRAAKFLADKKTGLYDMQDVLGFK"
     gene            complement(18593..19615)
                     /gene="bamE"
                     /locus_tag="PKL42_00325"
     CDS             complement(18593..19615)
                     /gene="bamE"
                     /locus_tag="PKL42_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HNU65539.1"
                     /translation="MRILSFLRYPIILLALLCTACGTALPTVKPFKLDVQQGNVVTSK
                     MLLQLRPGMTKSQVRFIMGTPLIQDSFHGNRWDYVYQMRESGKITEQRRVILDFENEL
                     LKSVRGDVAPSASDKATTESAAPNAGPRTINPTPKPQEKGMLDKLKFWKKDEAAPAIE
                     TKPAETKSGDTKPAEDVKQTLEAFEPPVATESIDMTAPAELTPAPQEEVKSILAVPIE
                     GITLPAPVIETPAAPERAPVERKPAVAPAEIEPTKVAPEKVVPPAPTVEEIKPVEKAV
                     KKAIETPKASTAAEALPAKADTKPIVKEVAKPKPVPSPIEPDMSDLPDEGSPTFFDKM
                     LEKIGF"
     gene            19730..20155
                     /gene="fur"
                     /locus_tag="PKL42_00330"
     CDS             19730..20155
                     /gene="fur"
                     /locus_tag="PKL42_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HNU65540.1"
                     /translation="MHDPSDLKNAGLKATLPRLKILSLFENSKDRHLSAEDIYKVMIG
                     AGEDVGLATVYRVLTQFEQAGLLIRHHFESGKAVFELNGGNHHDHIVCIKCGRVEEFY
                     DAEIEKRQKVAAEKFGFEMQDHSLTIYGVCTKQPCGGKD"
     gene            complement(20302..21456)
                     /locus_tag="PKL42_00335"
     CDS             complement(20302..21456)
                     /locus_tag="PKL42_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNU65541.1"
                     /translation="MNSTQTATPTLTWQEAGKTKTSAWQSENNAPTPKRIQIADDTIK
                     ADDAYKLCCEGTALLWRGDFQNAKHLLQALSRRIDRPGKKPKKAASTMLEAFHLHRQA
                     QSQKARILGMLVIELSVGYNINLRRAPDVKAACEHAYGKSTENIIVSLREILGLIGAY
                     EWHKNGVDIPAINNKIYPSYGVFSPIRGEYLDLVDTAQLPVPCNLAFDIGTGTGVLAA
                     VLANRGITKVVATDNSHRALDCALFNINQLGLKNNVALVEADLYPKIDYGNADLIVCN
                     PPWLPAHPSSALESAVYDEKSKMLKGFLNGLNARLSKTGEGWLILSDFAEHLGLRTRE
                     ELLSWIENAGLTVIDRIDTKAKHSKTLDASDPLHAARKAEVTSLWRLGRI"
     gene            complement(21522..22436)
                     /gene="prmB"
                     /locus_tag="PKL42_00340"
     CDS             complement(21522..22436)
                     /gene="prmB"
                     /locus_tag="PKL42_00340"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNU65542.1"
                     /translation="MTMYAQATQELHTIRDWLRFAVSQFENSDIFYGHGTDNSYDEAV
                     WLIMSALHLPMETLNNFLDARLTKPERDKLAQFIEQRITQHTPTAYLLKEAWLQGFKF
                     YVDERVLIPRSFIAELLANDLSPWIEFPEMIESAVDICTGSGCLGVLLANSFPNAAID
                     VIDISQDAIDVCNINIANYGLENQVTAIKSDMFSALKDENHVCKKYDIIISNPPYVDA
                     PSMAALPAEYQNEPQLALGSGTAGLDHTHTLLREAANYLNNDGILIVEIGHNRDALEA
                     AYPNTMFNWLETSSGNEFVFLLTKDQLN"
     gene            complement(22433..23572)
                     /gene="dapE"
                     /locus_tag="PKL42_00345"
     CDS             complement(22433..23572)
                     /gene="dapE"
                     /locus_tag="PKL42_00345"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HNU65543.1"
                     /translation="MSKTLELAIDLLKRQSNTPEDAGCQELMISRLEPLGFNIERMRF
                     GNVDNFYARRGTSAPLIVFAGHTDVVPTGPLDKWHTPPFDPTIKDGMLYARGAADMKT
                     SLAAFITSIEEFIAENPNHAGSIGLLITSDEEGVAVDGTVKVVEALKARGEHFDYCIV
                     GEPTSNKVVGDMIKNGRRGSLSGKLTVKGVQGHIAYPHLVKNPIHLVAPAIKDMVETV
                     WDNGNEYFPPTSWQISNMNGGTGATNVVPGEVEILFNFRFCPELNGAGSTEANLKERV
                     HSILDQHALNYDLEWEYSPPYITPRGNLVDAIEEAIQIAYGIKPELSTTGGTSDGRFI
                     ADICKQVIEFGPLNATIHKLNECVGVADIEPLKETYKLTMEILLK"
     gene            complement(23590..23934)
                     /locus_tag="PKL42_00350"
     CDS             complement(23590..23934)
                     /locus_tag="PKL42_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HNU65544.1"
                     /translation="MQLFGIPNCNTVKKARDWLASHQIAYEFHDFKKNGISQTLLENW
                     LTQRPWEKMVNRAGMTWRNLSDAEKAAVIDAQSAIQLMLAKPSVIKRPVLVKNGNIIG
                     LGFDEATYKELF"
     gene            complement(24159..26288)
                     /locus_tag="PKL42_00355"
     CDS             complement(24159..26288)
                     /locus_tag="PKL42_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNU65545.1"
                     /translation="MSHYNHELIALAIAFSYFAALMMLSIINKLNKSLSHDKKALLIC
                     SALIIGSSLWAIQLINAFALPSSSDMQSGTSSIAYIALSWLAAFIFAAILLYITSRPT
                     LPTKTLSAGGTLAGASAGAIVFFSIYANHAEAAMTFTPATSLIAIATCVVAAIIGMML
                     IFWLKNQHGKFSFLAKSMLALMVAFTAIGVHLTYSSAVATGSTAENANIAQFNSSLGL
                     ICLFLVGVLITVFYEKFSNNPLSFTKTNLPESLPVAFEAPTVDMSQLALIDTLTQLPN
                     RYALMQHLETATKRSQRNGNSLAVAFIDVDNFKPINDTHGHKVGDEVLQKIAKRLVTA
                     VRGCDEVARIGGDEFIAIIQDVEHDEDCIAIVERMVHSVRESCIINHTEMHLSVSVGV
                     AIYPKCGDIDELISAADTAMYRAKKDGKNQFRFFDDEIALASDQLIEFQYDLRNALAN
                     DEFVLHYQMKIDSITRMPIGAEAFLRWQHPIRGLLQPPEFMEAAERFGLSYAINNWVN
                     EECCRTLQELQALNIPFTIAINLSHQQIINPNLVKDMTEMLERFNLPTSSVIFELTEA
                     AAIKNQSLFHAQLARFKAANIKVAIDDFGTQSSSIANLQNLQVSELKLDPTFTANIEN
                     NHKIRSIIQAIIELAHVLELNVVAESIETEDQRRILAELGCDHMQGYLFSRPLPRDRL
                     ISLLKNLNLRFEESGVFFAKNIEEAVH"
     gene            complement(26557..27384)
                     /gene="dapD"
                     /locus_tag="PKL42_00360"
     CDS             complement(26557..27384)
                     /gene="dapD"
                     /locus_tag="PKL42_00360"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HNU65546.1"
                     /translation="MDPRQSIIEAAFEERANINPANASAEIKTTVASVLADLDAGTLR
                     VASRIADSQQWETHQWLKKAVLLSFRLEDNVLLDDGVTKYYDKVPPKFANYTEDDFKA
                     GGFRVVPNAIVRRGSFIAKNTVLMPSYVNIGAYVGEGSMVDTWATVGSCAQIGKNVHL
                     SGGVGIGGVLEPVQAGPTIIGDNCFVGARSEVVEGVVVEDNCVISMGVYIGQSTKIYD
                     RETGEVFYGRVPAGSVVVSGNLPSKDGSYSLYCAVIVKKVDAKTLGKVGINELLRGI"
     gene            complement(27507..28325)
                     /locus_tag="PKL42_00365"
     CDS             complement(27507..28325)
                     /locus_tag="PKL42_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNU65547.1"
                     /translation="MKKIAFVFFALVSLQLSGCATTTKDSVSGVKRSQFMLLPSGTVD
                     KMSAQAYTESLQTAKKKNTLNADTAQLERVRTISNHLIAQVGVFRPDAVQWNWEVNVE
                     KDDQVNAYCMPGGKIMVYTGLIEKLNATDDELAAVIGHEIAHALREHGRERMSQAYVQ
                     QFGLQALSAFVTKGAAGGAIMQGAGMGSQLFFALPNGREQERESDKIGLELAARAGYN
                     PDAAVTLWQKMGAQSKNSPPEFFSTHPASENRIAELKALAPKVRPLYEAAKQTK"
     gene            complement(28439..30322)
                     /locus_tag="PKL42_00370"
     CDS             complement(28439..30322)
                     /locus_tag="PKL42_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HNU65548.1"
                     /translation="MSSPTGSKSKLSVLTLAALGVVFGDIGTSPLYTIKEVFSVSSHP
                     VPLTEANMYGILSLIVWALIMVVSVKYVAFIMRADNRGEGGIMALLALASQSAEGDAK
                     KRKIIMTLGILGACMFYADGMITPAISVLSAIEGLELAAPILHPMILPITLIVLFGLF
                     WTQSKGTALVGAFFGPVMLIWFGTLGVLGLLGIMQNPAILHALNPIYGIKFFAVSPWI
                     AFVALGAVVLAVTGAEALYADMGHFGRIPVRLAWFGFVLPALILNYFGQGALVLLDPS
                     SIKNPFYLLAPTWMLYPLIILATMAAVIASQAVITGAFSVSRQALQLGFLPRMHVEHT
                     SENEEGQIYMPRVNWGLMLAVMVLVISFQSSGSLAAAYGIAVTGDMVITTLLAGFVFH
                     SIWGWSKLRTGALVILFLTIDIAFFSANVLKIPDGGWVPLVIGMIIFTLMLTWKTGRT
                     MVYTRLKNEAMELAPFIEAIGAHPPTRVSGSAIFMTPNPDGVPHAMLHNLKHNKVLHE
                     KVVILTVKFLDYPHTTPEERVSLETLPHEFYKVTVRYGFKDDPDLPRDLVSCREQGLV
                     LEAMDTSYFIGKEILIASEKFGMANWRKKIFIGLFRSAETITNQFKLPPNRVVELGSQ
                     LKI"
     gene            complement(30601..31821)
                     /gene="xseA"
                     /locus_tag="PKL42_00375"
     CDS             complement(30601..31821)
                     /gene="xseA"
                     /locus_tag="PKL42_00375"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNU65549.1"
                     /translation="MTEPSLLLNQKVLTVTELNRLAREVLEISFPLFWVSGEVSNFTR
                     AASGHWYFSLKDAGAQVRCVMFKGRNNYVDFVPREGDKIEARATVTLYEARGDFQLTV
                     EFVQRAGLGMLFEAFEKLKAKLSAEGLFEATRKQAIPEHPTKIGIVTSPDAAALRDVL
                     TTLKRRNPAISVIIYPTPVQGKGAAEMISQAINTAHERAEVDTLIICRGGGSIEDLWQ
                     FNEEIVARAIANCSIPTISGVGHETDFTMSDFVADVRAATPTAAAELACADQQSIKNQ
                     LTNLKNQLHKQMQLTLNQHAQQLDFLARRLISPAQQITNKTEQLAQLKLRLALSLQRQ
                     LQTHQQHLLRLKNSLAHLNPQAVLTRGFAFVQNQSGDIISNANQLHTGEAVSLTFGTG
                     AADATIDKITPPNR"
     gene            31874..32377
                     /locus_tag="PKL42_00380"
     CDS             31874..32377
                     /locus_tag="PKL42_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HNU65550.1"
                     /translation="MKIEKITKFLQGRTGYLLGFIASLGLVGLALWIQTKYNLDPCPL
                     CITQRIVFMVLGVCFLVAAIVNRYPKFFAGVQVLTALVGAGWASRHWWIQAHKETMIA
                     DCGVGFDYMFENFPLKKALSLVFKGTGDCAAIDWTFLGLTIPQLALIAFIGMGVYAVL
                     LVKFNQK"
     gene            32391..33278
                     /gene="cysM"
                     /locus_tag="PKL42_00385"
     CDS             32391..33278
                     /gene="cysM"
                     /locus_tag="PKL42_00385"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HNU65551.1"
                     /translation="MFKTLDQFVGNTPLVQLQRMGGNSSNTILLKLEGNNPAGSVKDR
                     PALSMIARAEARGDIKPGDTLIEATSGNTGIALAMVAAMRGYKMVLVMPENQSVERRQ
                     SMRAYGAELVLTPKDGSMELARDVALKMQAEGEGIVLDQFGNMDNPQAHYDGTGPEIW
                     RDTQGKVTHFVSSMGTTGTIMGTSRYLKEQNPDIKIIGVQPEEGAQIPGIRKWPEAYL
                     PKIFDAKRVDELIFVGQKQAEDATRKLATMEGIFAGISSGGALYAALQLSKRVENATI
                     VSIVCDRGDRYLSTGVFPA"
     gene            33295..35151
                     /locus_tag="PKL42_00390"
     CDS             33295..35151
                     /locus_tag="PKL42_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65552.1"
                     /translation="MTLLLWVFPQQSFAISRVEIHAQTITYDQQATAYDAHATIDLKH
                     HAVVDVQAKKIEYNQFEVRNALLHLDHQANAKISASAEVKQKKDKQWAKAKLLCVVPQ
                     RLQTDTWHCESGQYQAAQIDLPFSLHLTPQPNGFKANLNLKEANFSDEAGLHAAEKLS
                     GDLELNLTKEAHGYRWQNTLNWAGGEMFWQPFYFIGGGHKLSASGVLNEHAVTFEAST
                     LHIKNVGDIAFSGKIRRSDFHLITLDADMPNLDLGEAYPMIFKPLLEKTAFNNADIGG
                     KAALKVAVRDAEIKTFELKLQDVNIDDKNKKFAFYNINAQLPWSYDEAKKVSLAYSGG
                     HLLNLPLGAANLHAEVNRYSLISPNLTLPILDGALKLSDVSAARIGTDWYWHLQAQLI
                     PVSMADFSRALNLPIMQGKASAEIPLVTYSAGNLTTDGEMVLNVFDGTATITNLTMRN
                     PLGIAPRLNADIALRHLDLGNLTRTFSFGAIEGKLDGDIDDLQLQNWKPVKFDAEVHS
                     SPGKYAKKISQRAVENISALGGAGAAAAIQRSFLQFFKQFNYEKIGLSCKLRNDICEM
                     NGIASTDGGYIIVKGSGIPAITVLGYNHSVGWGELLARVKRVIDGNTKAVVK"
     gene            35196..35399
                     /locus_tag="PKL42_00395"
     CDS             35196..35399
                     /locus_tag="PKL42_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65553.1"
                     /translation="MKNWLMVISIAAMLPACVTINIYFPAAAAEKAADKIIDDVWQLK
                     NGEKPTVKTIEPSAEPAPADSAK"
     gene            35423..35842
                     /locus_tag="PKL42_00400"
     CDS             35423..35842
                     /locus_tag="PKL42_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HNU65554.1"
                     /translation="MKKIILSLVMVLSVLLASQFAVAAADLEVNTPAITAIKSSMQAR
                     HGQLAGFYASGAIGLTKDGLIAVKDATAVPLKDRGALNGLVSAENADRKNLYKEIATA
                     NGHPEWQGEIQSTFAGRWIDKAQSGWFYQGAGGWAQK"
     gene            35913..36689
                     /locus_tag="PKL42_00405"
     CDS             35913..36689
                     /locus_tag="PKL42_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNU65555.1"
                     /translation="MTPTLVFDIETIPDTAGLRTLLDVPAHATDEDIANIAFHHRRQQ
                     NGSEFLPLHQHKICAISCALREGNHFKVWTLGDANSTEAEIIQRFFDGIEKYTPQIIS
                     WNGGGFDLPVLHYRAMINKVQAPRYWDLGEDDKEFKWNNYISRYHTRHLDLMDLLAMF
                     NGRANAPLDEIAQLCGFPGKLGMDGSKVWNAYLAGEIDAIRNYCETDVANTHLVYLRF
                     QLMRGHLTPVAYEAEVHLVRDTLLSYSAPHWAEFLAAWKP"
     gene            36913..38298
                     /gene="rlmD"
                     /locus_tag="PKL42_00410"
     CDS             36913..38298
                     /gene="rlmD"
                     /locus_tag="PKL42_00410"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNU65556.1"
                     /translation="MARRSRSRNQAPIIPEIKFATIESLDQEGRGVAHVEGKTVFIDG
                     ALPQEKVSYQSHHIKASYEIANVVQVLKQSNQRVTPKCPHFGLCGGCKLQHLDFAAQV
                     AAKQRLLENDLKHIGKVKPDNILPPLFGPTWGYRHKARLSVKYVEKKQRVLVGFNEKA
                     TRYVADMNSCEVLVPEVSQLIVPLQNLIVQLSIRDKLPQIELAVGENPANVRESAHVI
                     VLILRIMDALNANDEDLLKSFADEHHVQIWTQTKGPDTIKPFYPLSAAQLQYSLPEFG
                     LTYPFKPNEFTQVNPQINQVMIRRAMQLLAPKAGENIADFFCGIGNFTLPIARSGAQV
                     LGLEGLANLVERANESAALNQISNTQFGVADLFKMTPEALTQLGRFDKWLVDPPRDGA
                     IELVKALDANNRPQKIVYVSCNPATLARDAGMLVNEKGYTLIAAGVINMFPHTAHVES
                     IALFELTGKTE"
     gene            38530..40611
                     /gene="ppk1"
                     /locus_tag="PKL42_00415"
     CDS             38530..40611
                     /gene="ppk1"
                     /locus_tag="PKL42_00415"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNU65557.1"
                     /translation="MPSTFPPLELLNRDTSILAFNERVLSLAQNANYPLLERLRFLCI
                     VSSNLDEFFEVRAAPHVEAMRDGIRQSDINVQSYQAIAEDAHLLVEKQYQIFNGELMP
                     TLAKQGVHLVSHGDRTAAQRRWVSRYFESDVRPLLVPVALDPSHPFPQVANKSLNFIV
                     ALSGQDAFGRSNNIAIVRVPRALPRLIKLPKNLSDKQQKFVSLSSVIRAHLASLFDGR
                     EVLNFSQFRVTRHSDLAVDEEDVKNLRTALRKGLVQRNFGDAVRLEVSHACDEKLAKF
                     LLQQFDLPKQSLYRVNGLVNLARLMQLADLAEGSNLKFKPFEPKISQQLVPNQSIFGQ
                     LKQRDILIHQPYESFESVIDFLREAVNDPDVVSIQQTIYRTGSDPRMLKLLQEAVRRG
                     KEVMAVVELKARFDEEANINWAEALESVGAQVVYGIVGLKTHAKMLLVMRREGVGLNG
                     STKLTHYGHVSTGNYNPRTAKLYTDVSMLTACPKITRDMEYLFRHIASQVKLPRLQKL
                     LVAPFNLHRQMLSKIKSAELAAKAGKQAHIILKMNALTDEVLVVALVKAGRAGVNIDL
                     IIRGACILPVDAPGLEGRIRVRSIIGRLLEHSRVFYFNVQDVEQMWLSSADWMNRNMM
                     RRVEIAWPIVDAENRARILQECCQLSLDDTQDAWQLLAGGVYQPVASLARKNHEHAFS
                     AQRTLIQQYGE"
     gene            40628..41107
                     /locus_tag="PKL42_00420"
     CDS             40628..41107
                     /locus_tag="PKL42_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNU65558.1"
                     /translation="MENLICWRHAEAEADSKTGADIDRELTKQGRKDAAAMAKWLHQH
                     LPANTEVLCSPARRCLETAAALQRFNRQQKVTIAEYLGVDNTVDKITKHVLNEDSSRT
                     ILIIGHQPNLGLLIARLLKMENEACVVKKGAVWWLRQRHVDDTVQTYLFTVQHPDFV"
     gene            complement(41140..41583)
                     /locus_tag="PKL42_00425"
     CDS             complement(41140..41583)
                     /locus_tag="PKL42_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65559.1"
                     /translation="MSKINAIQAAALKTTVAKTASTKPVTAKPVTAKPASKSTIAIKE
                     KPVKPATTTPVAKSAAKKAPKAAKPVKEKIPKPKMERDSFTIPKAEYAQLYVLKERLA
                     KLGAPAKKSELLRAGIMQLTALSDAALKTALSKVPTIKTGRPKKK"
     gene            complement(41659..43179)
                     /locus_tag="PKL42_00430"
     CDS             complement(41659..43179)
                     /locus_tag="PKL42_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HNU65560.1"
                     /translation="MENHTPIPADKSLLAAIDLGSNSFRLEIGRLDSGHIERVEYLKE
                     AVRQGGGLDEDRNLTPEAIERGLKCLARFGERLKGFDAAHVRAVATQTLREANNREVF
                     TKLAKKTLGFDVEVISGVEEARLIYQGVSRFLPQSNEKRLVIDIGGRSTEFILGQGFT
                     AHHTASLHVGSVAWSLKHFASGEFTEKAFTRAEIAAESIFETLGAHFNPQQWDVAYGA
                     SGTVGAVADVLVQYGRPIDTVTKEGLHWLREELLNTKHVEKLKLLGLKEDRKPVIAGG
                     LAILIGIFDYFQIETLHVAKGALRHGLLYDMVARENAMLDIRNASVLRLARKFDVDET
                     HANTVAEVADTLFDKISADIQFESGEQQAHARKLHWVALLHEIGSIVSPIDAHLHGAY
                     ILEHTEPAGFSQHELHCLSLLILGQKEKLKRLEADFSDRIFVMQLVCLRLSIILCHAR
                     KKPNLRGFQLRFLNGHIVLSLPPSWPDKFPQSYYLLEEERLAWLKINWRFDISANS"
     gene            43524..45452
                     /gene="htpG"
                     /locus_tag="PKL42_00435"
     CDS             43524..45452
                     /gene="htpG"
                     /locus_tag="PKL42_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNU65561.1"
                     /translation="MSAETAQSEIQPKQSMAFQAEVKQLLQLMIHSLYSNKEIVLREL
                     ISNASDAADKLRFEAISNGALYENDSELKIRVAFDKTARTVTISDNGIGMSRDEVIAN
                     IGTIAKSGTKEFFNALSGDQAKDANLIGQFGVGFYSAFIIADKVSLTTRRAGSSEAVR
                     WESSGEGDFTIEPAEKASRGTDVVLHLRENEDEFLSDWKLKTIIRKYSDHITLPIVMK
                     KSEWRNDEVSAEDKASAEVPTDEDETVNKASALWARAKNDITEEEYQEFYKHVSHDFE
                     NPLAYTHSRVEGKQEYISLLYIPGKAPFDLYDRDRRHGIKLYVKRVFIMEDAEKLMPQ
                     YLRFVRGVIDTSDLPLNVSREILQSSRDVDAIKAGSVKKVLGLLEDLAENKPEDYAKF
                     YAEFGRVLKEGPGEDFANKDKIAGLLRFASTKADTDVQDVSLKEYIARMQPEQELIYY
                     ITADSFAAAQHSPHLEIFRKKGIEVLLMSDRVDEWLLGSLTEFEGKKLQSIAKGDLDL
                     GKLESDTEKEIQKKIEEEAKSLVEKIKQALGEQVKDVRVTHRLTDSPACLVSDAHDLS
                     GNLARMLKAAGQKAPESKPILEINPTHKLVKRLEGETAEALFGDLAHVLFDQALLAEG
                     GQLDDPASFVKRMNSLIS"
     gene            45758..46675
                     /locus_tag="PKL42_00440"
     CDS             45758..46675
                     /locus_tag="PKL42_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65562.1"
                     /translation="MTMLNIQRASIVYGIILQFLTPGRFIKSLGFLMLAGMPLMAHSE
                     GYDRLPEAIHIVFVSMVYVFASRAVYWFVGAAIFAWVLSFLLRLYTKSDNAYKNSSAE
                     QHQPVNNVVGLLVMVGWGLMFLAVLALIVVGIGRSMGFKDVSTLPKTRYQPIQISKEE
                     RERQASLIPKVVDDMSWKIYSPLNKPWPANEGALENERQEGARGGKYDVAIYKTSTSG
                     SIFFKLCYVVTGRCDGLRHAYMLNQARYTFQNVEDGEYEVRMLNLKTGSAWRSKPFTV
                     PRSSPVKVVAAFQLGDGIKEGTPVAPEDF"
     gene            46832..>47365
                     /locus_tag="PKL42_00445"
     CDS             46832..>47365
                     /locus_tag="PKL42_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNU65563.1"
                     /translation="MKTPAKTSDKAVAKTKTATKAKTDTGAAAKPKKTAKAKRVLPKL
                     SPVDQALQNHLIFSSFKTNDAATPRDWYDAASYTVRDHVVARWIKTAEAYYEKDPKRV
                     YYLSLEFLMGRMLSNAALNLGIQDELKDGLASLGRELENTVEMETDAALGNGGLGRLA
                     ACFVDSMATMDIPATGYG"
BASE COUNT        12846 a        10267 c        11526 g        12726 t
ORIGIN      
        1 gtatcgccta ttcaaagaaa atgatttggc gctattagaa attaacccac tggtggtgac
       61 aaccgatggc aagttgtttg cgctagattg caaaatgagt attgatgata acgccttgta
      121 tcgccaaaaa gccttagctg aacagcgcga ttggtctcag gaagacagca aagaggcgat
      181 tgcccatcat gctgggttga attacatcgc cctaaacggc aatatcggtt gtatggtgaa
      241 tggtgcaggc ttggcgatgg caaccatgga tttgattaaa ctgcatggcg gcgcaccagc
      301 caacttttta gatgtgggcg gtggcgcgac ggctgaaaca gtcacaaagg ccttcaagat
      361 tattctggaa gatcataacg ttaaagcgat tttggtcaac atttttggcg gcatcatgcg
      421 ttgcgatatt attgccgaag gcattatcac cgctgtaaaa gaagtcggca tgacgattcc
      481 ggttgtcgtc cgtttagagg gaacaaacgt tgagttgggc aagcaaatgt tgcgcgaaag
      541 cggcttaaat attatttcag cagaaaactt aaccgatgcg gccaaacaag ccgtcaactc
      601 tgttaaaggt caggcagtat gagcatatta gtcgataaaa acaccaaagt tttatgccag
      661 ggctttaccg gcaagcaagg cacgtttcac tctgaacaag cgctggctta cggcactaaa
      721 atggtgggcg gcgtcacgcc aggtaaaggc gggcagttgc acttaggttt accagtgttt
      781 aacaccatgc gagatgctgt gcgtgatacg caggctaacg ccagcgtcat ttacgtgcca
      841 cctgcttttg ccgcagactc cattttagaa gcaagtgacg caggtattga gctgattatt
      901 tgtattactg aaggcattcc agtgctggat atgctcaacg taaaggccgc acttaaagcc
      961 aatggcacca aactgattgg ccccaattgc ccgggcatta ttacgccaga tgaatgcaaa
     1021 atcggtatta tgccaggcag tattcacttg cgtggtaagg tgggcgtggt gtcacgctct
     1081 ggcacgttga cgtatgaagc ggtgcatcaa accaccgcct tagggcttgg tcaaaccacc
     1141 tgtgtgggta ttggtggcga cccgattaaa gggttaaatt ttattgagtg tttaaccatg
     1201 tttgaggccg accctgaaac cgaagcgatt attatggtag gtgaaatcgg tggttcagat
     1261 gaagaagcgg cagcagaatt cattaaaagc catgtcacaa aaccggtggc ggcctatatt
     1321 gcggggcaaa cagcgccagc tggtaagcgt atgggtcatg cgggggcgat tatttcaggt
     1381 ggcagcggta aggccgttga taaaatacgc gcgttggaac gcgctggtgc tgtagtggca
     1441 agttcccctg ctggcttggg cgaagccgtg cttgatgcaa tcaaagctaa caaaaaggta
     1501 gcataaacaa tttatgaaaa cattcctcaa gcgttttgcg ttcattttac tgctcacgac
     1561 catgccggta atggctgccg aagatattgc ctatttactc acggcgtcgt ccaagggcga
     1621 tttggcaacg gtcaatgcct tacttgccag cggcgctaac ccaaatgcga aagacggtga
     1681 gggcattact gcgctgatgt acgcagcacg caaagataag gcagaggttg ccgcaagcct
     1741 cattgaaaag ggtgcggaca tgaatgcggt tgacaatggc ggttggacgc cattgatgta
     1801 tgcggcaaag aaaaatcata ttgcaaccat cgcggtgcta cttgaaaaag gcgctgaccc
     1861 aaaaattcgt gataccgcgg gctggagtgc gcttggtttg gcagcatcat caggtttttc
     1921 acagagcgtt gatttactca taaaacacgg cattgatgtg aacgctaaaa gcgatagcgg
     1981 aaaaacggtg ttaatgtatg ccgcaaaaaa tggcgacatt ccaaccattg atattttatt
     2041 ggcgaacaaa gcagacccta gagcccaaga taaattgggt gcgacagcat taatgattgc
     2101 cgcacgtgaa ggccatgcac cggttgtgac gttgttggca aaagcgggga cgaatgttaa
     2161 ccagaaagac cgtgctaaat ggacggcact gacttgggct gtgaaaaaat ctaacattga
     2221 agctgctgct gtattggtag aggctggggc tgatgtcaat aatcaggatg cagaaggtac
     2281 gccgttgcta catgtggcgg tggatagtgg caaagccgaa attgtgaaat tattgattga
     2341 gcatgatgtg aacgtgaagg ccaaagacca atatggtcta accgctttgg tgtatgcctt
     2401 aaaaggccag cacactgaaa ttgcaaagat gattaaagcc gctggcggca agtattagtg
     2461 gccaacgcag catgaaaatt gccatcgcgc aaatgaactg tatagtcggt gatattgccg
     2521 gcaatgtggc acaaatcatc gcatactcgc agcaggccaa ggcgcaaggc gcgagcttag
     2581 tcgtgacccc agagttgtca ctttgcgggt atccaccaga agatttactg ttacgggatg
     2641 attttttaca ggcttgcgca cgtggactca agcaattatg tgaagaaaca caaggtctaa
     2701 cgttgattgt aggccatcct catcaagtgg gtgagttttg ctataacgcg gcctcagtca
     2761 ttcaacacgg taaactatta gcaacttatc ataaacactc actgccaaat tacagtgtgt
     2821 ttgatgaaaa acgttatttt gcagcgggcg agcatgcgct ggtttttgag catcaagatg
     2881 tcaacatcgg tgtgctgatt tgtgcggatg tttgggaacc tgcacccgcc ctaaaagcaa
     2941 aagctgcggg cgcacaatta ttgattgcgc tcaatgcatc gccatttcat attgaaaagc
     3001 atcgtacgcg tttagaggtt ttacgtgagc gcgtggctga aaccaacttg cctatcatgt
     3061 acatcaatat ggtgggcggg caggatgaac tggtttttga tggtggatcg tttgtgttaa
     3121 gcgacacggg cgcgatcaca catcatttaa atgcttttga acaagcgtta gccatcgtcg
     3181 attgccaagc agccatgcca ctcacaggaa aagttgcacc gcatttaagt ttggaagctt
     3241 cggtgtacca ggccttaacg ttaggcttac atgattacgt gcgtaaaaat ggttttcctg
     3301 gtgtggtatt aggcttgtct ggtggtattg attcggcttt aacccttgcc attgcggtag
     3361 atgcactagg cgcagagcag gtgcatgccg tgctgatgtc atcggaattt accgctaata
     3421 tgagcgtgga tgatgccgtt gaaatggcga atattcttgg ggtaaaacac agtttaattc
     3481 cgattgtgac gctatttgag catttcagaa gtgcgctttc gacagaattt gcaggtttac
     3541 cgttcgatac cacagaggaa aatctacagg cgcgcattcg cggcatgttg ctaatggcac
     3601 tctctaataa atttggcagc attgtggtca ccactggcaa caaaagtgaa atggccgtgg
     3661 gctactgcac actttatggc gatatggcgg gcggctttgc tttgcttaaa gatgtgccaa
     3721 aaaccttggt gtacaaactt agccaatacc gaaatagcct atcgcaagtg attccgcagc
     3781 gcgttattac gcgcccacct tcggctgagt tgcgagaaaa tcagcttgat caagatagct
     3841 tgccgcctta cgaaatattg gatggtatta ttgaagccta cgttgaggat gatttaagcc
     3901 gtgcagatat tattgcgcaa ggttatcaag cagcagatgt aagccgcgtg atgaaattga
     3961 tagaccgaaa tgagtacaaa cgccgtcaag cgccagtagg cgtgcgtatt accgaccgtg
     4021 gatttggtaa agaccggcgt tatcccatta ccgttaaatt ggattttgat acacttttta
     4081 ctgaataaat tttattgaat caaattgatt gcatcaattt ggcaaacaat cgtaattctt
     4141 aaggatgact cacatgaaaa aaattgaagc aatcattaaa ccctttaagt tggatgaagt
     4201 acgagaagct ttatctcaaa ttggcgtgaa tggcctgact gcaactgagg tgaaagggtt
     4261 tgggcgccaa aaaggccata ccgaacttta tcgcggtgct gaatacgtgg tcgactttct
     4321 acctaaaatt aagcttgaac tcgtgttacc tgaaaatctg gttgattctg ccattgaagc
     4381 cattattaaa gcggcacaca ctggaaaaat tggcgatggt aaaatttttg ttagtgcggt
     4441 agagcaagtt atccgtattc gcacaggtga aacaggcgaa tctgcagttt aaggcgttta
     4501 tatactaggt agtatttttt tcaatgtgaa gcgtcggtta tgttaatcaa aaaggggtca
     4561 agcgtacgca cttaaccccc tttaatgatg ctactagtgc tattagcgaa cggaaattcg
     4621 ctttcctgca atcgttattt tttttggtag cgttaattcc agcacaccgt tctcataaga
     4681 cgcttcagca tgctcttgat caacatccac aggtaattga aaacttcttg aaaccgcacc
     4741 aaaataacgt tcactacgca caacctgacc atcttcagtt ttctgatcgt gctgtttaat
     4801 ttctgctgcg attgtcacag tgctgccatc caccgttaaa ttgaggtctt cctttttgac
     4861 cccaggaatt tcagcatgaa acacataagc tttatcggta tctttgatat ccactttaaa
     4921 agaactagcg accccttcac cgtgaagtgg gctgacaaaa tagccttgcg tacggcggtc
     4981 gccaaaaaaa tcattcagta atgcgttatt aaatcttgcg agttgagaca tcatttactc
     5041 cttcaaaaaa ataaattgtg atgaaaaaac tgtcaatatc ccctaaggcc taaattgggt
     5101 gtggttaaaa tttttcaagt gaaaaatatg ttttttgtag aaaatatcca cgagcaaaaa
     5161 acgctgccct gcgagcctta taaataaagg ctcgcagggc agcgtaaaag ggttggggtg
     5221 aaaatttact taattgagct gatttttttg taaccaagac caagcgccat tgcccgcaac
     5281 acgtccactg gcaaagcagg cggtgagcaa gtagccgccc gtcggcgcct cccaatccaa
     5341 catttcaccg gcacagaaca cccctggtaa tttggtcagc attaattctt catttaagtc
     5401 ggtaaatgtt acgccgcctg cactgctgat ggcttcatcc aatgggcgtg tggaattgag
     5461 tacgataggc aatgctttga tggcgttgcc aagttgcact gggtcattca ttgcttcggc
     5521 cgtgagcacc tctctaagca gtgcaacttt cacgcctttt aaattgagcc ggctttgaag
     5581 gtggcttgac agcgagcgtg caccacgcgg atgcgcgatt gcttgaatga ccgcatcgat
     5641 atttttttga gggagtaaat ccaaatgtat ttgtgcatgg ccattggcct caatttcatc
     5701 gcgtagcgtg gctgaaatcg cataaatcag gctaccttca acaccgttag acgtgaccat
     5761 aatttcgcca gatttttcat gaatggcatc atgcttgtct gcatagttaa cgctaatggt
     5821 tttaagcggt gcgccagcaa aacgtgcttg aaagtagtca ctccagctaa cgttaaagcc
     5881 gcagttggcg ggcttaaggg ttgccacttt gatgccttgt tgcgcgagta atggtagcca
     5941 ttttccatca gaacccagtt tggcccaact gccgccgccc agcgccaaaa tgacggcatc
     6001 aggtttggtg aagtaagcac cttgtggcgt gtgaaagcat aagttttgtt gtgcatccca
     6061 accttgccat tggtggcgca catgaaaatg caccccagcg tgacgtaatc ggtgtagcca
     6121 ggcgcgcaat aaaggggcgg ccttcatatc tgttgggaac acgcgccctg atgacccgac
     6181 aaacgtttca aagccaagtt gatgcaccca gtcacgcagg cttgcggcgt ttaattgttc
     6241 gagcataggg gcgatttgat tttgcctatc accataacgc gtgatgaatt gtgcggctgg
     6301 ttcagcatgg gtgatattca taccgccaat gcctgccatt aaaaatttac ggccaacgct
     6361 gggcatggca tcatacacat gcacatcaac agattgcgca atgagtgttt ctgccgccat
     6421 caggccggcc ggcccagcgc cgatgatggc aaccgtgtta gaagtttttt tgttcaacgt
     6481 cgttttattg ggtgaaggat gctggcgtat tataaggcga atgagtgcat tgtaaaaaat
     6541 ttacggcgct aaatttaata ccactggaat cttaaaaacg ctctatttaa aaccgctcgt
     6601 atttaaaacc ctaatgcgcg cgcaccctga taaaacacaa agctaaccac ccaggcaaga
     6661 ctcagcgacc acacaattga tagccacata aaagcactgc tttttgattc acttctcaag
     6721 gttgccactg tggataaaca aggcgtgtag atgagggtaa acagcataaa actcatggct
     6781 tgcacccaat caacttgcgt tgccatttgc accatgagtg cgtctccttg catgccgtaa
     6841 atcacggcga gtgaacccac cacaatttct tttgctacaa agccaaaaat cagggcaatg
     6901 gccagttggc tgttaatgcc gatggggtcg agtattgggg caaacaaagt accaatttgc
     6961 ccggcatagg tgccgctact ggcaggtgga acatcactag gaaagttcgt caatacccaa
     7021 accagtacca caccaatgat gatgaatttg gttgcgcggt taagaaagtg tttgacttca
     7081 tgccagccac gcaacacgat ttgccgcaag gtaggaaaac gatacggcgg caattccagt
     7141 atgaacggct cagtattttt gtactggcct tgaaacaaca ccgccgttaa aaacatggtg
     7201 gcaaagctca tgatatacag cgtgaacaac acaaaaggcg cttgtttggc ggtaaagagc
     7261 gcggtaatca taaaaatgaa tacttgtaat cgcgcagaac aaagcgagag cggaatgacc
     7321 agcatggtga gtagccgcat cggtcttgaa cgcataatgc gcgttcccat cagggccggt
     7381 acgttgcagc caaagcccat cagcatcatc acaaaaccgc ggccatctaa gcccattttc
     7441 gccatgagcg catccatcag aaaagctgcg cgtgataaat aaccactgtc ttctaccatc
     7501 gacatgacca aaaaaaacag aataataatc ggcacaaaag ccgccactgt ggccacacca
     7561 ttaaaaatgc catccagcat aagcccgctt aaccaagccg gactgttggc aaaaagcggc
     7621 tcaagggctg cggtgcgcag taaatcaagc gcccaagcca agccgtcttg caatggcttg
     7681 ccagcggtga aaataaactg aaataacaag aatatcgccg caaaaaagat ggggagccct
     7741 aaccatttgt gtagcaaaat acggtcaatt ttatcggtgg tgttgtcgct cagcgtggcc
     7801 ggaatctgca catgatgcgc aatcaattgc tccatctcgc gctcgatttg attgtcttct
     7861 tgcaaaagtt ggctgatttg ttgcgggtca gtggcggttt tgttttggtt gataatggtg
     7921 gttgccgctt gcaaagcttt tggcaggcca tcaccaaatt ttgcactgat ggaaatcacc
     7981 ggcatgttga gttcgcgcgt caagctttca gtgtcaatgg tgatacccgc gcgtttggcc
     8041 tcatcggcca tattgagcgc cagcacggcg ggtaaattaa gcttttggat ttgtaacacg
     8101 agtgaaagct ggcggtcaat ttgcgagctg ttgagcagaa taatgactaa atctaccgca
     8161 ttattggcta aaaaatggcg gacgacttgt tcgtcttcag aaaagccgtg caaatcataa
     8221 atgccaggta aatcaatgag ttcagcaata tgcccgccca ttaaaatttt agcgctcatt
     8281 aaatctacgg taatgccagg ccaattgcct acgcgtgctg atgcaccgct gatgcgatta
     8341 aacagggtgg atttccccgt gttgggcatg cccaatagtg cgatacgttt catgttagag
     8401 cgtattaata cgaatgcgcg cggcttcggc agcgcgtaaa atcacatccg ttgtgcctag
     8461 gcgcacttgt aatggtcctt taaaattggc ccgccgaata atactgagcg gtttgcccac
     8521 tctaaaccca agtgcagata ggcgatgaaa cagcgactca tctgcttcaa tggcagaaat
     8581 caccgcaggg gcaccaatat tgagctgtga taataattgc atattgtcaa tcttactttt
     8641 ttggtaaatg ataattattc ttattattgc acataataaa taaaaaatga aagcctgttg
     8701 cggtaatatt ttagcaacgg catcacaaat caaaacttga gtatggcatc gttgtgatga
     8761 gacgcatttg acctaacgca tcgtttagtc aggtttttag gtaaagtttc tagcagagct
     8821 agatagggta tttaggcact taaagtaagt gaaaaatatt gtttaatttg gctaaatgca
     8881 acgcgaggaa ttttttatgc cctgtgagcc tttatttata aggcttgcag aggcatcaaa
     8941 aaggttgggt gttaaaacag ttgtttttga tgatttttta tggctatttt aaagctactt
     9001 ttaaacaaaa aaaggactcg atttcgagtc ctttctgtgc gcaataactg ccttaaaact
     9061 tatttggcaa tatcaacttt agctttgctg cgtaagtcag ccatcatttt ttctaagtta
     9121 cgttgttgca gatttttttg taagccatct ttaactttgt cgtaagccaa tggttgtgct
     9181 gcacgcgagt cgattgactt aatgacatgc cagccaaatt gcgtttgtac tggctcggcc
     9241 gttgtttttc cttttggtaa ggctgctaca gcatcgctaa aaggctttac catcgttgca
     9301 ggtgagaacc agcctaaatc gccgccttta tcttttgaac ctgggtccag tgatttttct
     9361 ttcgctgttt ttgcaaaatc accgcctttt tgtaatttag cgataatttc tttggcttct
     9421 gcttctgttt ttaccaaaat atggcgagcg ctgtattctt tgtcgccata ggcttttttg
     9481 tattcttcat acgcggcttt ggtgtcagct tcagaaattg ggtttttctt cacaaaatct
     9541 tgcaaatagg ctgaagtgag taattcacgg cgtgagagtt cttcacgtgc gatgtaatct
     9601 gattgtttat caagacccag tttaacggct tcttggtaga cgatttctga atccactaat
     9661 ttattggtga tggccgcttt gacttggtca tcaatttttt ggccgcgcgc agaagcatct
     9721 ttggcaatgt agtcgtaaac agatttgttg atgcctttac cattgacggt ggcaatggtt
     9781 tcagcagcaa atgcggcagg ggctaacgtg ataattgccg ctgcaattag ggtttgggta
     9841 aatttcatgc aagattcctc atggataaaa attaaaatta aaattaaaaa attaggattg
     9901 gttaagcttc acttggcgca agtgcttgta tgctcaaggc gtgaatttta ttaggaatta
     9961 agtcggcaac cagttgataa ataagacgat gccgcattat ctgcgatttt tgagaaaaat
    10021 gcgagcttgt tattttaagt ttgaaatgtc caccacctgt attcctcgca tgaccttggt
    10081 gttgggcgct ttcatccata atttccagct gggttggttc taagctttgc aggcgatggg
    10141 tgatttcagt gattaaattc attaggcttt agtgacggta acgtattagt tagggtagcg
    10201 ttttgcgaaa aggtttcaca atcacattcg cataaacgcc agcttgtaca aatggatcat
    10261 cgtttgccca tgtgtttgca gcagtaaggc tagaaaattc agccacaatt aaactgccgc
    10321 taaagcctgc aggtcctggg tccgtgctgt caatcgcagg aaaaggccct gccaataaca
    10381 aacggcccgc ctcttgcaac gcggtgaggc gagctaaatg tgctggccga gcgcttaggc
    10441 gtttttcaag actgttcggt gtatcttcag cgataatcgc ataccacatt acttagtgtc
    10501 ctctttcggc aaatatttat tgagcatgag cgtttgtaaa ataataaata caaacataat
    10561 gcctgtggcg ccaaacagct taaagctcgc ccaagtgtca atagagtaat taaaagctac
    10621 gtataaattg agaacgccta acaatataaa aaacaccgcc caagaataat ttaatttttt
    10681 ccaaaccagt tctggcagtt ccatcgcgtg gcccatcatg gctttgatga ggtttttatg
    10741 ccaaaacaaa tctgctgcaa ttagcaccac agaaaatagc cagtaaagca cggttggttt
    10801 ccatttaata aaaccgtcat tttgaaaata taaagtcatg ccaccaagta aaacaattag
    10861 cgctgcgcta atgagcaaca tgttgtcgac tttgccatgc ttaaatttaa cccaggccac
    10921 ttgaattacc gtagcaataa tcaccgcaac ggttgccagc atcaccggca ctaaatcagg
    10981 ctttacggta accgccaagc caactgccga aaaaatttct gccaaccaaa cgctggctga
    11041 ggcggttttt tcagccactt ttaaggtgat aaagaacatt aacaccggaa ataaatcaaa
    11101 caggaatttt ttcatgggct atcacttatt gggtgagcgt aataataaaa atgggtgcgt
    11161 taaaaacatc aatgtataac agtgctttag cgaaaagcgt tattttgcta tgatagctaa
    11221 gatgccgcaa gggataattt caacgcttaa caataagtga ttgcgcattt aatttacgtt
    11281 tgattggact tatgtcgtta aaaattgatt tacattgcca ctcgaccatt tcagatggca
    11341 tgctcgcgcc tgaagacttg gtggcttatg cggcaagaca aggcgtgaat gtgctggcat
    11401 tgaccgacca tgatgatgtc ggcggcctga aagtagcaag acaagcagcc gatgaacacg
    11461 gcattacctt gattaatggt gttgaaattt ccgtcacttg gaaaaagcgc acgttgcata
    11521 ttgttggttt gaaaatcaat ccagacaacg aaaccttaaa aaatgccttg aatcaagtgc
    11581 atatcgaccg tgatgcccgt gcaagagaaa tggcagaaag ccttgccaaa gccggcattc
    11641 cagaagccta cgaaggcgca aaagccatcg ccaagcaatc aataatcacg cgtgtacatt
    11701 ttgcccgctt tttggctgaa aaaggctatg caaaagatac caaatcagtc ttcaaaaaat
    11761 atctggtcaa aggtaaacct gggtatgtga atcatcagtg gatgacttta gagcaagcgg
    11821 taaaacttat tttagacagt ggcggcattc ctgtcattgc gcatccgggc cgttatgatt
    11881 taggcatcat caatatgctg ttgttgatgc atgagtttag aagttacggc ggcgtggcga
    11941 tagaagtggt gacgagtagt catacgccac cgcaatacca gcaatttgca aaacttgcgc
    12001 atcagtttgg gttgaagtcg tcgcaaggtt cagattatca cgggcaaggt atcagtttta
    12061 tggaaatggg ctatttgccg ccattgccag ccaattgcat acctgtctgg caagattggc
    12121 ctgaagtgca agaggttact gcctaaacca tgtcgcaact atttaatatt catttagaaa
    12181 atccgcagcc gcgcttaatt acgcatacgg ctgaaatttt acgagatggc ggtgtgattg
    12241 cgtacccaac agattccggc tacgcgctgg ggtgcatgtt gggttttggc gatgcacaag
    12301 cgcgtattcg ccaaattcgc ggtgtggatg ataaacattt atttacgctg gtgtgccgca
    12361 atttgagcga gctgggaaac tatgcaattg tgagcaacag ccaatttcgt cttctcaaag
    12421 ccaatacgcc tggcgcttat acttttattt taaatgcaac aaaagaagtt ccgcgcaaat
    12481 tgcagcatcc aaaacgtagc gcggtgggta ttcgcgtgcc gcaacatccc attgtacaag
    12541 cgatattaga agccatcgat gcgccacttt taagcatgac attgcatatt gcaggggatg
    12601 atgaagtgct gagcgaagca tggaaaatcc gcgagcggct agagcgtttg gtcgatttgg
    12661 tgattgatgc gggtcactgc acggcagggg ctacttcggt aatcgatttg actgcagaag
    12721 caccggtgtt gattcgtgca gggcagggcg atttggcgcc ttttgggcta gatgcggtag
    12781 attgatgcgt cagaatgacg caacacctta actggttaaa attttaaaag agaaaattag
    12841 agaaaatgga cttatcaatc atacagaaaa ttattattta tgcgctgccc attatatttg
    12901 ccattaccgt gcatgaggct gcacacggct atgctgcaag gcattttggc gacatgacgg
    12961 cttttaatgc tggacgcatt accctaaacc caatcaaaca tattgaccct ttcggcacca
    13021 tcttactacc tgctttaacg gtcatgttag gcggtatttt atttggctgg gcaaagcctg
    13081 tgccggttaa ttttggcgcc ttgcgtaacc ctaaaaaaga catgctatgg gttgcggctg
    13141 cgggccctgc ttcaaatttt gtcatggcgg ttttttgggc aatcatgttt aagttggcca
    13201 ttggcgcgcc agaagccatt gccgtgccac ttgcactgat ggcaaaagca ggagtgagca
    13261 tcaatattgt actcatggta ttaaatttat tgccgctacc accgctggat ggcggccgta
    13321 ttgcggtgag cttattgcct catcatcttg cacggccttt tgcgcaaatt gagcgctacg
    13381 gtttttttat tttaatcgca ctacttttta cgggcgtgtt aggcatggtg ctgggtccgc
    13441 tggtgaactt tgcctctaac ttcatctata gttttgttaa cttgttataa tcatcacaca
    13501 ttacaattaa ggttttttag cacatggcaa tcgaacgcgt tttatcgggc atgcgcccta
    13561 caggcaacct gcatttaggg cattacaacg gcgtactcaa aaactggata cgcctacaac
    13621 atgagcatga gtgtttattt tttgtggctg attggcacgc gctgacgaca cattacgata
    13681 cgcctgaaat cattgaagaa agcgtgtggg aaatggtgat tgattggctc gctgcagggg
    13741 ttgacccagc gaacgccacc atttttattc aatcgcgcgt gccagaacac gctgagctgc
    13801 ataccttatt gagcatgatt acgcctttaa gttggcttga acgcgtgccg acttataaag
    13861 accaacaaga aaagctatcg aataaagatt tatctactta cggctttttg ggctaccctt
    13921 tactacaaag cgcggatatt ctcatttaca aagccacgca agtacctgtg ggcgaagacc
    13981 aaattccgca tattgaattt actcgtgaaa tcgcccgccg ctttaaccac atttatggca
    14041 aagaaaaagg ttttgaagaa aaagccgaag ccgccattaa aaaactcggc agcaaacgtg
    14101 ccgcgcttta tgaagaaatg cgcaatgcct accaacaaag tggcgatgaa gaagcgctgg
    14161 aatcagcacg cgctatgata gaagagcaac aaggcatgag catgggcgac aaagagcgct
    14221 tgcttggtta ccttgaaggt ggcggcaaaa ttattttggt agagccagaa tataaactca
    14281 cgcctgcctc aaaaatgccg ggcttagatg ggcaaaaaat gtctaagtca tataacaaca
    14341 ccatttcatt gcgtgaaaat cctgagacaa tcgccaaaaa aatcaaaact atgcctaccg
    14401 accccgcgcg tattcgccgc accgacccgg gcgaccctgc gaaatgcccc gtttggcaac
    14461 tgcatgaaat ttattctgaa gacaatgtaa aagattgggt gcaaatgggc tgtaaaggcg
    14521 ccactattgg ctgcattgaa tgtaaaggcc cggtgattga aggcgtgtta aaagaactca
    14581 aaccgattca agagcgcgcc gcacaatatg ccgaagaccc gactttagtg aaaaatattg
    14641 tggcagaagg ttgtgaaaaa gcgcggaaac ttgcgcgtga gacgatgcgg gatgtgcgtg
    14701 atgctatggg tttgagttat agttaagagc ggtcattttg ttagaaacct ccatcaacgc
    14761 aaaaatcaaa ggcgaacttc tcaccgaact gccgcaagat ttatacattc cgcctgatgc
    14821 gctggaagtg tatttagagt cgtttgaagg tccactcgat ttgttgttgt atctcatccg
    14881 caagcataat ctggatattc tagatattcc tatggcggat ttaactcgcc aatatatggt
    14941 gtatgtggag aaaatgaaag cgattaagct tgaactggcg ggcgattatt tgctgatgtc
    15001 agccatgctc attgaaatta aatcgcgcat gttgttgcca aaacctgctg aaattgccga
    15061 agaagatgac ccgcgcgctg agttagtgcg ccgtttgatg gaatatgaag cgattaagct
    15121 agccgcgcaa gggctggatg aaatgcctaa agtgggcgaa gagttaagtg tggcggcggc
    15181 atattatcaa catatctctg agattaagcc gcctgaagtg agcttagatg aactggcaga
    15241 agcgtggcgc aatgtgttaa agcgcgccaa actgaatagc caccacaaaa ttggccgcgc
    15301 ggagttgtct gtgcgcgaac acatgagcca gattctacgc cgcttaaaaa ccgatggttt
    15361 ggtggagttt tcacagcttt ttgatatcgt gaatgatggt ttgcctaagc tggtggtgaa
    15421 ttttttagcc attttagagc tggcaaaaga aggcttaatt cgcattaccc aacaagaagc
    15481 tttttcgccg atttatttgc aggtgaatgg cgcagactga ttcttaagtt taacagtcgt
    15541 cattcccgcg taggcgggaa tccagttggg ttgagtaagg taaggcgaat tttactgaca
    15601 tggacaaaat tgaaaatcgc ctagattccc acatgcgcgg gaatgacgtg attttagatt
    15661 ggaagcaagt gaaagaaccc gaaaatatca tagaaatgaa gcaagtgctt gaagcggctt
    15721 tactgacatc agcccagcca ttgagtttgg atgatatgct caagctgttt gcagggcgca
    15781 tggagcgtgc cactttgcgc atgctattgg atgagttgaa ggtagattgg gagccgcgca
    15841 cgatggaact ggtgcaagtg gccacgggtt accgctttca ggcaaaagca gaatatagcg
    15901 aatttattgc gcgtttaaac cccgaaaagc agccaaaata ttcacgcgcg gtgatggaaa
    15961 cgctagccat tattgcctac cgccagcctg tgacacgtgg cgatattgaa gaaattcgcg
    16021 gtgtggcagt gaacccgaat gtgatgcgcc agctgcaaga gcgcgactgg attgatgtgg
    16081 tgggtcatcg tgaagtgccg ggcagaccat ctctatatgc aacgaccaaa cattttttag
    16141 atgatttaaa tttacgctcg ctggcagagt tgccgccaat ggaagttttt gcccagcaag
    16201 aaatgccgct cgatagttag tcatattcgc taatactgtt cgtgctgatc ttggtcgtta
    16261 aaatgccgca acggccaagc tcagggcgaa cgggttgata aataatccaa ttgccagctt
    16321 atgcggtaaa atgcatacca ttcaattaag ttagcacatc attatggcac gcccctttaa
    16381 aacccagcgc gacccacaag caacaccgcg tcgccccaaa accgatttca agaaaattga
    16441 aatcgatgaa aatgcgccac ccgcgcccac gcagcgccta cacaagctat tagcgttggc
    16501 tggcttgggt tcacggcgcg atatggagtt gttgatagaa agcggtcgtg ttacggtgaa
    16561 tggaaaacct gctgtggttg ggcaaggcgt gactgagcat gatgtggtgc gcttagatag
    16621 ccgcccactc aagttgcctt ttgaggcaga gttgccgcaa gtgctgattt accataagcc
    16681 agaaggtgaa attgtcagcc aagacgaccc tgaagggcgc gccagcgtgt ttgataaatt
    16741 gccaaaagta aagcacggca aatggattgc cattggccgt ttggatatga atacgagtgg
    16801 tttgctgatt tttaccacgt ctggcgagtt ggcaaaccac tttatgcacc cgcgctatga
    16861 agtggagcgt gaatacgccg tgcgtatttt tggtgaactg accgaaggcc aaatgacaca
    16921 attgactgaa ggcattgagt tggaagatgg cccagccagt tttgatagca tccgcccgca
    16981 aggcggcgag ggcgctaatc attggtatca agtaattttg cgtgaaggtc gcaaccgcga
    17041 agtgcgccgt ttgtttgaag cgtttcaact acctgtgagc cgcctcatgc gtgtgcgctt
    17101 tggtccggtg aatttgccgc cgcgtgtgaa gcgcggcact atgttaaaac ttgagcaaaa
    17161 agaagtggtg ggcttgttag agtgggccgg cttgcctgta ccaaatgcac cgttaaggca
    17221 actgacacag cgcgaaaaag acaaagccac cagcgtattt gtgcctaaag tacgcaaaca
    17281 gcgcgtgagt gcgttggata gaccaccgcg cgaagtcggt gagcgtactt cataccgtgg
    17341 caacaatgca ccgaattcag cgactaaaaa acctgcgctt aatagcaata aacccaaaag
    17401 acctgccaac cgccgagtgc gtcaagggag tgatttaaat gcgccaacca tgcagaaaaa
    17461 aagcgataga aatcgtgggc gcggttaagc attaactgct taaaacacac gttttttaac
    17521 tacaaccttt tttgatgctc tgagagcctt tatttacaag gcttccagag catttatttt
    17581 ctctcgggag attttagtgc agaaaagcac tttatttttg acgttaaagg ggacttaaaa
    17641 aattatttaa aaccgagtac atcttgcatg tcgtatagcc cagttttttt gtcggccaaa
    17701 aacttggcgg cacgtaatgc gcccagtgca aaggtggcgc ggctgctggc tttgtgcgtg
    17761 agttcaacgc gttcaccaat gcctgccagc accaccgtgt ggtcgcccac cacatcgccc
    17821 ccacgtagcg tggcaaaacc aatcgttcct gcctcgcgtt cacctgtcac accttcacgc
    17881 gcataaaccg cgcactcgct taaggcttta ccaatgccat gtgcagcggc ttcacccaag
    17941 cgtagcgcgg tgccagaagg cgcatccact ttatggcgat gatgcatttc aaccacttca
    18001 atatcgtagc cattcgccag taccttggcc gcttgctcca ctaaattgat gagtagcgtg
    18061 acacccacgc tcatgttggg cgcaaacacg atgctgatgt ttttggcggc ttttgcgatg
    18121 gtggcttttt cttcagcaga aaatcctgtt gtgccgatga ccaattttac tttggctttt
    18181 tcgcaagcgg caagatattg catactggct tcaggacgcg tgaaatcgac caacacatcg
    18241 gcatttttta acgcttcatc catgttatca gtgattttaa ccgctgaatt tttaccaaac
    18301 tgttcgccag catcacggcc aagttgcggg ttgcctgctc tatcgagcgc gccgtgcagt
    18361 ttgagatcag catcagcaaa tacgctttca agtaacacat gccccatgcg gccagtgcaa
    18421 ccagcgataa cgacttttaa agggtttgcc atcatttatg aatacctaat tttaaaaacc
    18481 aatcttttag ctacgacacc actcgcttcg cgagtaagtc tgcacctaga aatttaaaat
    18541 attcttttag ctacaacatc actcgcttcg cgagtgagtc tacgctgaaa gattaaaaac
    18601 caatcttttc aagcattttg tcgaagaagg tgggtgagcc ttcgtctggt aaatcgctca
    18661 tgtctggctc aattgggcta ggcacaggtt ttggttttgc gacttctttt acaatcggtt
    18721 ttgtatcggc ttttgcagga agcgcttctg ccgctgtgct ggccttgggt gtttctatcg
    18781 cttttttcac tgctttttca accggtttga tttcttcaac cgttggcgca ggtggaacca
    18841 ctttttcagg cgctactttt gtcggttcaa tttcggcagg cgctacagca ggcttacgct
    18901 caacaggcgc tctttcaggt gctgcaggtg tttcaatcac aggtgctggc agggtaatgc
    18961 cttcaattgg cacagctaaa attgatttca cttcttcttg tggcgctggc gttaactctg
    19021 ctggggcagt catgtctatg ctttcagtcg ctaccggtgg ttcaaaggct tcaagcgttt
    19081 gctttacatc ctctgccggt ttggtgtcgc ccgattttgt ctcagcaggt ttagtctcaa
    19141 tcgctggtgc ggcttcatct tttttccaga atttcagttt atccaacatg cctttttctt
    19201 gaggttttgg cgtggggttg atggtgcgcg ggccagcatt tggcgctgct gattctgttg
    19261 tcgctttatc gctggctgaa ggtgcaacat caccacgcac agattttaat aattcgtttt
    19321 caaagtctaa aatcacgcgg cgttgctcgg tgattttgcc gctttcgcgc atttggtaaa
    19381 cataatccca acggttaccg tgaaaactat cctgaatgag cggtgtgccc ataataaagc
    19441 gcacctgtga tttggtcatg ccggggcgca attgcaacaa cattttagac gtcaccacgt
    19501 taccctgctg tacatccagc ttaaatggtt taacggtggg tagtgcagtg ccgcacgcag
    19561 tgcaaagcag ggctaacagg atgatggggt aacgcaaaaa agataaaata cgcataatat
    19621 gactttaatt tgaataagcg ttaatataac acgttgacat tgaaccccca aaattctggc
    19681 accactttta cgggcttttt gccgttcatt ttattaagag cacacgcgta tgcatgatcc
    19741 atctgactta aaaaacgcag gactcaaagc gaccctgccg cggcttaaaa ttttaagcct
    19801 gtttgaaaac agcaaagacc gccatttatc ggctgaagat atttataaag tgatgatagg
    19861 cgcaggcgag gatgtgggtt tagcaacggt ttatcgtgta cttacgcagt ttgagcaagc
    19921 aggcttgctg attcgacacc attttgaaag cggtaaagcg gtgtttgaac tcaatggcgg
    19981 caatcatcac gaccatatcg tctgcattaa atgcggccgt gtggaagagt tttacgatgc
    20041 cgaaattgaa aaaagacaaa aagtagcggc agaaaaattt ggcttcgaaa tgcaagatca
    20101 ttcgctgacc atttacggcg tttgcactaa acaaccttgt ggtggtaaag actaaaaact
    20161 gttttttaac cttatttttt ctgatttagc tatgcatttt ctctcgagag aatattatgc
    20221 ctctggaagc cttgtaaata aaggatcgca gagcagctca aaaggttggt gagcaaaaac
    20281 acgtcaaaat gcgttttttc gttagattct tcccaaccgc cacaatgaag tcacctcagc
    20341 ctttcgcgct gcgtgtagtg ggtcgctggc atcgagtgtt ttgctgtgtt tggctttggt
    20401 gtcgattctg tcaatcactg ttaaacctgc gttttcaatc cagctcaaga gttcttcacg
    20461 cgttcttaag cctaagtgtt cggcaaagtc agagagaatc agccagcctt cacccgtttt
    20521 gcttaagcgt gcattgaggc catttaaaaa gcctttcagc attttgcttt tttcatcgta
    20581 cacggctgat tctaacgcgg aactcggatg tgcaggcagc cacggcggat tacagacgat
    20641 taaatcggcg ttgccgtaat caatttttgg gtataaatcg gcttctacca gcgcgacatt
    20701 gttttttaag ccaagttggt tgatgttaaa cagcgcacaa tctaaggcgc ggtgcgagtt
    20761 atcggtggca actactttgg tgataccgcg attagccagc acggctgcca gcacgccagt
    20821 gccggtgcca atgtcaaacg caaggttgca gggcacgggc agttgtgcgg tatcgaccaa
    20881 gtctaaatat tccccacgaa ttggtgaaaa cacgccataa ctagggtaga ttttattgtt
    20941 gatggcagga atatcaacgc catttttgtg ccattcataa gcaccgatga ggcctaaaat
    21001 ctcgcgtaat gaaacgatga tattttctgt gcttttgcca taagcgtgct cgcaagctgc
    21061 tttaacatcg ggcgcgcggc gtaagttaat gttgtagcca acacttaatt caatgactaa
    21121 catgcccaaa atacgtgctt tttgcgactg tgcctggcga tgtaaatgaa acgcttctag
    21181 catggtactg gccgcttttt tgggcttttt accgggccgg tctatgcggc gcgataacgc
    21241 ttgcaataga tgtttagcat tttgaaagtc acctcgccat agcaatgccg tgccttcgca
    21301 acagagtttg taggcgtcat cggctttaat ggtgtcgtcc gcaatttgta tgcgttttgg
    21361 ggttggcgca ttgttttcag attgccaagc gctggtttta gttttgcctg cttcttgcca
    21421 tgtaagggtg ggcgtagcag tttgggtgga attcaaaata gagtggcctg gtgaatggtg
    21481 cgtgagaata gggtaaaaat taatttaaat taactggtct tttaatttaa ctggtctttg
    21541 gtgagtaaaa atacaaactc attgccagat gacgtttcta gccagttgaa catggtgttg
    21601 gggtaagcgg cttccagcgc atcgcgatta tggccaattt ctacaatcaa tatgccgtca
    21661 ttattcaggt aattcgccgc ttcacgcaat agcgtgtgcg tgtggtctaa gcccgctgtg
    21721 ccgctaccaa gcgccagctg tggctcattt tggtattctg caggcagtgc tgccattgaa
    21781 ggcgcatcca catacggtgg attactgatg atgatgtcgt attttttgca gacatgattt
    21841 tcatctttta gtgcgctaaa catatctgat ttgatggctg tcacttgatt ttctaaaccg
    21901 taattcgcaa tgttgatgtt gcacacatca atcgcatctt gcgaaatatc aatcacatca
    21961 attgccgcat taggaaaact attcgccaac aacacgccta aacagccgct gccggtacag
    22021 atgtctactg cgctttcaat catttctgga aactcaatcc aagggcttaa atcattggct
    22081 agtaactcgg caatgaacga gcgcggaatc agcacgcgtt catccacata aaatttaaag
    22141 ccttgcaacc acgcttcttt gagcaagtat gcggttggcg tgtgttgggt gatgcgctgc
    22201 tcaatgaact gcgccagttt gtcgcgctct ggtttggtta ggcgcgcatc aagaaagtta
    22261 ttcaaggttt ccatcggcaa atgtagcgcg ctcatgatga gccaaaccgc ttcatcgtaa
    22321 ctgttgtctg tgccgtggcc gtaaaaaata tcagaatttt caaactggct gacggcaaag
    22381 cgcagccaat cgcgaatggt gtggagttct tgcgtagctt gtgcgtacat ggtcatttga
    22441 gtaagatttc catggtcagt ttgtaggttt ctttgagcgg ctcaatatca gccacgccca
    22501 cacattcatt gagtttatgg atggtggcat tcagtgggcc aaattcaatc acttgtttgc
    22561 aaatgtcggc aataaaacgt ccatccgaag tgccgccggt ggtggagagt tctggcttga
    22621 tgccataagc gatttgaatg gcttcttcaa tcgcatccac caagttgccg cgcggggtga
    22681 tgtagggtgg ggagtattcc cactccaaat catagttcaa ggcgtgctgg tctaaaatgc
    22741 tgtgtacgcg ctcttttaaa ttggcttctg tgctgcctgc gccattaagt tctgggcaaa
    22801 atctgaagtt aaataaaatt tccacttcgc ctgggactac gttggtggcg cctgtgccgc
    22861 cgttcatatt gctaatttgc caagaggttg gcggaaaata ttcgtttccg ttatcccaca
    22921 cggtttcgac catgtcttta atggcggggg caactaagtg gatggggttt tttaccaaat
    22981 gcggataagc gatatggcct tgcacgcctt ttaccgtgag cttgcctgaa agcgagccgc
    23041 ggcgaccgtt tttaatcatg tcgccaacca ctttattgct ggtgggttcg ccgacgatgc
    23101 agtaatcaaa atgttcaccg cgcgccttta aggcttctac cacttttacc gtgccatcca
    23161 ccgccacacc ttcttcatct gaggtaatca gcaggccgat agaacctgcg tggttgggat
    23221 tttcggcgat aaattcttca atgctggtga taaaagcggc cagtgacgtt ttcatatctg
    23281 ccgcaccgcg cgcgtagagc atgccgtctt taatggtggg atcaaatggc ggcgtatgcc
    23341 atttgtcgag tggacctgtt ggcactacat cagtatggcc tgcaaatacg attaaaggcg
    23401 cagaggtgcc gcgccgcgcg taaaaattat ctacattacc aaagcgcatg cgctcaatgt
    23461 tgaagcccag cggttcaagg cgtgaaatca tcaattcctg acagccagca tcttcaggcg
    23521 tgttggattg gcgttttaat aaatcgatgg ctaattcgag tgttttgctc atttgaataa
    23581 ttctttgcat tagaataact ctttgtaagt ggcttcatca aaaccaaggc caataatatt
    23641 gccgtttttc actagcacag ggcgtttaat cacggacggt tttgccaaca tcagctgaat
    23701 ggcagattgc gcatcaatga cggcagcttt ttcagcgtct gacagattgc gccacgtcat
    23761 gcccgcgcga ttcaccatct tttcccaagg gcgttgagtg agccagtttt caagtagcgt
    23821 ttggctgatg ccattttttt tgaaatcatg aaactcgtaa gcaatctggt ggctcgctag
    23881 ccagtcacgg gcttttttga cggtgttgca attgggaatg ccaaatagct gcataggatt
    23941 cgcgttaaaa tgtaaaagtg tattttaaca gcattacaga ttactgctga atttatgcaa
    24001 atgtgcctgt ttaaaaattg attataagca cactaaactt gtacgagaaa aaatatatgc
    24061 tctgcaagcc tttatttata aggcttgcag agcatcgaaa aaggttgggg taaaaatagc
    24121 attaaaaaga catgctttta ccccgataat tttttaaatt aatgaacggc ttcttcaata
    24181 tttttggcaa aaaatacacc ggattcttca aaacgtaaat tcaagttttt cagtaagcta
    24241 atcaagcgat ctctcggcaa agggcgtgag aataaatagc cttgcatgtg gtcgcacccc
    24301 agctctgcta gaattctgcg ttggtcttcg gtttcaatgc tttctgccac cacgttaagc
    24361 tcaagcacgt gtgccaactc aataatcgct tgaatgatgc tgcgaatttt atggttattt
    24421 tcaatattgg ccgtgaacgt agggtctaat ttcagttcgc tcacttgcag attttgcaag
    24481 ttggcaatgc ttgaagattg cgtgccgaag tcatcaatgg ccactttaat attggcggct
    24541 ttgaatcggg ccagctgcgc atgaaacagc gactgatttt taatggctgc ggcttcagtg
    24601 agttcaaaaa tcacacttga agtcggcagg ttaaagcgct caagcatttc agtcatgtct
    24661 ttcaccaaat tcgggttgat gatttgctga tgcgataagt taatggcaat ggtgaaaggg
    24721 atattgagtg cctgtaattc ttgcaaggtg cggcagcact cttcattcac ccagttatta
    24781 atggcatagc tcaaaccaaa gcgctctgca gcttccataa attctggcgg ttgcagcaag
    24841 cctctgatgg gatgctgcca gcgtaaaaag gcttctgcac cgattggcat tctggtgatg
    24901 ctgtcaattt tcatttgata atgcaataca aattcatcat tggccagcgc gtttcttaaa
    24961 tcatattgaa actcaatcaa ttggtctgaa gctaaggcga tttcatcatc aaaaaagcgg
    25021 aattgatttt tgccatcttt tttggcacgg tacatggccg tgtcagccgc gctgatgagt
    25081 tcatcaatgt cgccgcattt ggggtagatg gctacgccaa cagaaaccga caagtgcatt
    25141 tcagtgtggt tgatgatgca agattcacgt accgaatgca ccatgcgctc aacgatggcg
    25201 atgcagtctt catcgtgttc aacatcttga ataatcgcaa taaattcatc accgccaata
    25261 cgtgcgactt catcacaacc gcgcaccgcc gtcactaaac gtttagcaat tttttgcaag
    25321 acttcatcgc caactttatg gccatgcgtg tcgttaatcg gtttaaagtt atccacatca
    25381 ataaacgcta cggccaaact attgccatta cgttggctgc gttttgtggc ggtctcaaga
    25441 tgctgcatca atgcgtagcg gttaggcaat tgggtgagcg tatcaatgag tgccagttgg
    25501 ctcatgtcca cagttggcgc ttcaaaggca actggcaacg actcaggtag gttggttttg
    25561 gtaaaactga gcggattgtt gctaaacttc tcgtaaaaaa ccgtaatcag cacgcccact
    25621 aaaaacaggc aaattaagcc aagagagcta ttaaattgtg caatgttggc gttttcagcg
    25681 gtcgaacccg ttgcaactgc tgaagaataa gtcagatgaa caccaatggc agtaaaagcc
    25741 accatcagtg ccagcattga tttagctaag aaagaaaatt taccatgctg attttttaac
    25801 caaaatatca gcatcatgcc aatgatcgcc gcgaccacgc aagtggcgat ggcaattaaa
    25861 ctggttgctg gcgtaaatgt catggcagct tctgcgtggt tggcataaat actaaaaaat
    25921 acaatggcac ctgcgctggc accagccaat gtgccgccag cagagagtgt tttggtgggt
    25981 agggtggggc gactggtgat gtagagcaaa atggcagcaa aaataaacgc agcgagccat
    26041 gaaagtgcta tgtacgcgat ggacgacgta ccagattgca tgtcgcttga actcggcagt
    26101 gcaaaagcat taatgagttg aatcgcccat aagctgctac caataatgag cgcgctacag
    26161 atgagcagag cttttttatc atgcgaaagc gatttattga gcttattgat gatgctcagc
    26221 atcattaacg ctgcaaagta gctaaacgca atcgccagtg cgatcaattc atggttgtaa
    26281 tgactcattt tattttttaa attatctaaa tggaatattc agataatccc tataagtaac
    26341 tagatgatag agtcattgca atgtctatgc catgcatcaa ggacgcgttt gcaatagatg
    26401 tgaaaaaggc tgcagaagcg cttatttgct tgcttgcagc cttttttatt ggttttgcta
    26461 cgttgcggtt gttcgtcgtt ttaaagcaaa aatgtcaaaa ttacaacact tatgttcagg
    26521 tatgttcacg ctgtgacgcg tcaacagtcg cgccaattaa atgccgcgta ataactcgtt
    26581 aatgccgact ttacccaagg ttttagcatc tacttttttt acgataaccg cgcagtaaag
    26641 actgtagcta ccgtcttttg aaggcaagtt acctgaaacc acaacagaac cagccggtac
    26701 gcggccgtag aatacttcac ctgtttcgcg gtcataaatt ttagtgcttt ggccaatata
    26761 cacacccatg gagatgacgc agttatcttc caccaccacg ccttccacca cttcagaacg
    26821 tgcgccaaca aagcaattgt caccaataat ggtaggtccg gcctgcactg gctctaatac
    26881 accgccaatg cctacgccgc cacttaaatg cacgtttttg ccaatttgcg cgcatgaacc
    26941 aaccgtcgcc caagtatcaa ccatagagcc ttcgcccacg tatgcgccaa tatttacata
    27001 agacggcatt aacacggtat tttttgcaat aaaagaaccg cggcgcacga tggcattggg
    27061 caccacacgg aaaccaccgg ctttaaagtc atcttcagtg tagttggcaa attttggtgg
    27121 cactttgtca tagtatttcg tcacaccatc atccaaaagc acgttatctt ctaagcggaa
    27181 tgacaacagc acggcttttt tcaaccattg gtgtgtttcc cattgttggc tgtcagcaat
    27241 gcggctggca acgcgcaatg tgcctgcatc taaatcagct aagacggatg caacagtcgt
    27301 tttgatttca gcgctggcgt tggctgggtt gatgtttgcg cgctcttcaa acgcggcttc
    27361 gataatgctt tgacgtgggt ccatggggct ctctgatgaa aggaaaataa acaaaacgtt
    27421 attttaacct atatcaagaa atcgccaaaa acaaaaagcg cgttattgaa acgcgctttt
    27481 tggtaactat cgattaagtc gcaaacttat ttggtttgct ttgctgcttc gtagagcggc
    27541 ctgactttag gtgcaagtgc tttaagttca gcaatgcgat tttcactggc ggggtgggtg
    27601 ctaaaaaatt caggcggtga atttttagac tgcgcgccca ttttttgcca taaagtcacc
    27661 gcagcgtctg gattgtagcc tgcacgtgcg gccaattcta acccaatttt gtctgattca
    27721 cgctcttgtt cgcgcccgtt aggtagggca aaaaagagct ggcttcccat gccagcgcct
    27781 tgcataattg cgccgcccgc cgcgcctttg gtgacaaacg cagaaagcgc ctgtaggcca
    27841 aattgttgta cataggcttg tgacatacgc tcgcgaccat gctcacgcaa ggcgtgggcg
    27901 atttcatggc caatcactgc tgcaagttca tcatcggtgg cattgagttt ttcaataagg
    27961 ccggtataca ccatgatttt gccacccggc atgcagtagg cgttgacttg gtcgtctttt
    28021 tctacgttca cttcccaatt ccattggact gcatcaggcc taaagacgcc aacttgtgca
    28081 ataaggtggt ttgagatggt gcgaacgcgc tctagttgcg ctgtgtcggc attgagggtg
    28141 tttttctttt ttgcagtctg taaggattct gtgtaggctt gggctgacat tttatcgacc
    28201 gtaccggaag gcaacagcat aaattgtgag cgctttacgc ctgaaacact gtctttggtg
    28261 gtggtggcgc aaccgctaag ttgtaacgag actagggcaa aaaatacaaa ggcgattttt
    28321 ttcatgagcg attccttaat gcaaaaatat cacaaagcgc aataaattac gttaacgtat
    28381 gtacgctgat ttggttgaac gtgaaaaaac tatattttaa attgcgagcc aagcttagtt
    28441 atattttgag ttgcgaacca agttcgacaa cgcggtttgg tggtaattta aactggttgg
    28501 taatcgtttc agcacttcta aataagccaa taaagatttt tttgcgccaa ttggccatgc
    28561 caaatttttc gctggcaatg aggatttctt taccaatgaa atatgaagtg tccatggctt
    28621 ctaacaccaa gccctgctca cgacaactga ctaaatcacg aggcaaatcg gggtcatctt
    28681 taaagccata gcgtactgta actttgtaaa attcatgcgg cagcgtttct aggctgacac
    28741 gttcttcagg ggttgtgtgc gggtaatcta aaaactttac tgtcaggatg acgacttttt
    28801 catgcaacac tttattgtgc ttgaggttgt gtagcatagc gtgcggtaca ccgtctgggt
    28861 taggtgtcat gaaaatggca ctgcccgaaa cgcgcgttgg cggatgcgcg ccgatggctt
    28921 caataaaggg ggcaagttcc atagcttcat ttttaagccg ggtgtagacc atggtccgac
    28981 cggttttcca tgtgagcatt agggtaaaaa taatcatgcc aatcactagt ggcacccaac
    29041 cgccatctgg gatttttaat acattggcgc taaaaaaggc gatgtcgata gttaaaaata
    29101 aaatcaccag tgcgccggtg cgtagtttgc tccaacccca tatggaatga aacacaaaac
    29161 ctgccagcaa ggttgtaatg accatgtcgc ctgtgaccgc aattccataa gcggcggcca
    29221 aactgccaga agattgaaag ctaatcacta acaccatcac ggccagcatc aacccccagt
    29281 tcacgcgcgg catgtagatt tgcccctctt cattttcaga agtgtgctca acatgcatgc
    29341 gcggtaaaaa gcccaattgc aaggcttggc gtgacaccga aaatgcgcca gtaatcacag
    29401 cctgtgaagc aatcacggcg gccatggtgg ctaaaataat caatggatac aacatccacg
    29461 tgggggcaag caggtaaaaa ggatttttaa tgctgcttgg gtcaagcaac actaatgcgc
    29521 cttgcccaaa ataattaaga ataagcgccg gcaacacaaa gccaaaccaa gcaaggcgaa
    29581 ctggaatgcg tccaaaatgg cccatgtccg catataaagc ttcagcgccg gttaccgcca
    29641 gcaccaccgc gcctagcgcc acaaaagcaa tccaagggct gactgcaaaa aacttaatgc
    29701 catagatggg gttgagtgca tgcaaaatag cagggttttg cataatgccc aaaagcccaa
    29761 gtacgcccag cgtgccaaac caaatgagca taacggggcc aaaaaaagca ccgacgagtg
    29821 cggtgccttt actttgcgtc cagaataagc caaatagcac gatgagggtg atgggtaaaa
    29881 tcatggggtg caaaatcggt gcggcgagtt ctagcccttc aatcgctgac agcaccgaaa
    29941 tggccggtgt aatcatgccg tcggcataaa acatacaggc acctaaaatg cctaaggtca
    30001 tgatgatttt gcgttttttg gcatcgcctt cagcgctttg gcttgccaac gccagcaagg
    30061 ccataatgcc gccttcacca cggttatcgg cacgcatgat aaaggccaca tatttaactg
    30121 aaaccaccat aatcagcgcc cacacgatga gcgacaaaat gccgtacatg ttggcctcag
    30181 tcagaggcac gggatgcgag ctgaccgaaa aaacttcttt gatggtatac agtgggctgg
    30241 tgccgatgtc gccaaacaca acaccaagtg cggctaaggt gagtacggaa agtttggatt
    30301 tactgccagt aggagatgac attaaaaatc aatacgcaaa atgacaagaa cgcaattatc
    30361 taccgaatta aaagcaaaac caatcataaa gtgtaaatat tcttaaaaat atcgttgcaa
    30421 aaatgcaaaa atgcacaaaa aaataaagca atttacgcac gttttcaccc cgagaaaaaa
    30481 ttacgcctct ggaagccttt atttataagg cttccagagc atcgaaaaag gttgtgatta
    30541 aaaacgggtt aaattaagtg ttttttagga acatttcggg tgttggagtt gcaccagatg
    30601 ttagcggttt ggtggcgtaa ttttatcaat ggtcgcgtcc gctgcgcccg tgccaaaagt
    30661 aagtgacacg gcttcgccgg tatgtagctg gttggcgttg ctgatgatgt cgcccgactg
    30721 attttgcaca aaggcaaagc cgcgcgtgag cactgcctgc gggttaaggt gagcgaggct
    30781 gttttttaag cgcaacaagt gttgctgatg ggtttgtaat tgccgttgca aactaagcgc
    30841 gaggcgtagt ttaagttgcg ccaattgttc tgttttattg gtaatttgct gcgcggggga
    30901 gattaatctg cgcgctaaaa aatccagctg ttgtgcgtgc tggttaaggg ttagctgcat
    30961 ttgtttgtgt agctgatttt ttaagttggt cagctggttt ttaatgcttt gttggtctgc
    31021 acacgccagt tcggcggcgg ccgttggcgt ggcggcccga acgtctgcca caaaatcgct
    31081 catggtaaag tcggtttcat gccccacccc gctaatggtt ggaatgctgc aattggcgat
    31141 ggcgcgcgcg acaatttctt cgttaaattg ccataaatct tcaatactgc cgccaccgcg
    31201 acaaataatg agcgtgtcaa cctcagcacg ctcatgcgcg gtgttgatgg cttgactaat
    31261 catttcagcc gcacctttgc cttgcacagg tgtggggtaa ataatgacgg atatcgcagg
    31321 gttgcggcgt tttagcgtgg taagtacatc gcgcagtgcg gctgcatcgg gcgaggtgac
    31381 aatgccaatt ttagtgggat gctcagggat ggcttgcttg cgcgttgctt caaataagcc
    31441 ttcagcgctc agtttggctt tgagcttttc aaaggcttca aacagcatgc ccaaaccagc
    31501 gcgctgtaca aattcaaccg tgagttgaaa gtcgccacgc gcttcataca gggtgaccgt
    31561 ggcacgcgct tcaattttat cgccctcacg cggaacaaaa tcaacataat tattgcggcc
    31621 tttaaacatc acacagcgca cttgcgcgcc cgcgtctttg agcgaaaaat accagtgacc
    31681 gctggccgca cgcgtgaagt ttgatacttc gcccgacacc caaaacagtg gaaaactaat
    31741 ttctagcact tcgcgcgcta aacgattcag ttcggtaacg gtcaatacct tttggttaag
    31801 caataaactt ggttctgtca tggggcttta tctattacaa tgtttaattg ttcgattata
    31861 agcgggttaa atgatgaaaa ttgaaaaaat cacaaaattc ttgcaaggta gaacaggcta
    31921 tcttttaggc tttattgcaa gcttaggctt ggtgggcttg gcgctatgga tacagaccaa
    31981 atacaacttg gacccatgcc cattgtgcat tacgcaacgc attgtattta tggtgttggg
    32041 cgtatgcttt ttagtggcgg caattgtgaa ccgttaccct aaattttttg caggcgtgca
    32101 agtgttaacc gcgttagtgg gcgcaggctg ggcaagccgc cattggtgga ttcaagcgca
    32161 taaagaaacc atgattgccg attgcggtgt gggttttgat tatatgtttg agaattttcc
    32221 gctgaaaaaa gccttgagtt tggtgtttaa aggcacgggt gactgcgcgg ccattgattg
    32281 gacttttctt ggactcacca ttccgcaact ggccttgata gcatttatcg gcatgggcgt
    32341 ttatgcggtg ttgttagtta aattcaatca aaaataatat tgagatatac atgtttaaaa
    32401 cgttagacca atttgtgggt aacacgccgc tggtgcagct gcaaagaatg ggtggaaatt
    32461 ccagcaatac cattttgtta aaactagaag gtaacaatcc agcgggttca gtgaaagata
    32521 ggccggcact ctcaatgatt gcgcgggctg aagcgcgggg tgacattaag ccaggtgaca
    32581 cgctgattga agccacgagt ggtaatacgg gcatcgcact cgccatggtg gcggccatgc
    32641 gcggctacaa aatggtgctg gtcatgcctg agaaccaaag tgtggagcgc cgccaaagca
    32701 tgagagccta cggcgcagag cttgtgttga cgcccaaaga cggcagcatg gaattggcac
    32761 gtgatgtagc cctgaaaatg caagcggagg gcgaaggcat tgtgctcgac caatttggca
    32821 atatggataa cccgcaggcg cattatgacg gcactggccc agaaatttgg cgcgacacac
    32881 aaggcaaagt cacacatttt gtttccagta tgggcaccac tggcacgatt atgggtacgt
    32941 cgcgctattt aaaagagcaa aatcctgata ttaaaattat tggtgtgcag ccagaagagg
    33001 gcgcgcaaat tcccggtatt cgcaaatggc cagaggccta tttgcctaaa atttttgatg
    33061 ccaaacgtgt ggacgaactc atttttgtgg ggcaaaaaca ggcggaagac gccacgcgca
    33121 aactagccac gatggaaggt atttttgccg gaatttccag cggcggcgcg ctttatgcgg
    33181 cattgcagct ttcaaaaagg gttgaaaatg ccaccattgt gagcattgtg tgtgatcgtg
    33241 gcgaccgcta tttatctaca ggcgtgttcc ccgcataata tgaggtgggt agtggtgact
    33301 ttgttgttgt gggtgtttcc gcagcagagt tttgccatta gccgggttga aattcacgcg
    33361 caaaccatta cttatgatca acaggccacc gcgtacgatg cacatgcgac gattgatctc
    33421 aaacaccacg cagtggtgga tgttcaggca aaaaaaatcg aatacaatca atttgaagtg
    33481 cgtaatgcac tgctgcattt agatcatcaa gccaacgcta aaatcagtgc ttcagcagaa
    33541 gtaaaacaaa agaaagacaa acaatgggcg aaggctaaat tgctttgtgt agtgccacaa
    33601 cgcttacaaa cagacacatg gcattgcgaa agcgggcaat atcaggcagc acagattgac
    33661 ttacccttta gcctgcattt aacgccgcag cccaatggtt ttaaagccaa tctcaattta
    33721 aaagaagcca atttcagtga tgaagcaggc ctgcatgcgg ctgagaagtt atctggcgat
    33781 ttggaactta acctgactaa agaagcacat ggctatcgtt ggcagaatac gctgaattgg
    33841 gctgggggtg aaatgttctg gcagccgttt tattttattg gtggcggaca caagctaagt
    33901 gcctctggcg tgttgaatga acatgccgtg acgtttgaag catcaacgct acacatcaaa
    33961 aatgtaggag atatcgcatt tagcgggaaa attcggcgta gcgattttca tctcatcacg
    34021 cttgatgccg atatgcctaa cctagattta ggcgaagcgt atccaatgat ttttaagccg
    34081 ttgttagaaa aaaccgcctt taataatgcg gatatcggcg gtaaagctgc gctaaaagtt
    34141 gccgtgcgtg atgcggaaat taaaactttt gagctcaaac tgcaagacgt gaatattgac
    34201 gataaaaata aaaaatttgc tttttacaac atcaatgccc aactgccttg gagttatgat
    34261 gaagctaaaa aagtgagcct cgcttacagt ggcggccatt tgctgaattt gccgctcggg
    34321 gcagccaatt tacatgctga ggtgaatcgc tattcgctga tatcgcctaa tctaacgttg
    34381 cctattttgg atggcgcact caaacttagc gatgtttcgg ccgcacgtat tggtaccgat
    34441 tggtattggc atttgcaggc gcaattgatt ccagtatcca tggcagattt tagtcgcgca
    34501 ctcaacttac ctattatgca aggaaaagcc tcagcggaaa tccctttggt gacttacagt
    34561 gcaggcaatt taacgacgga tggtgaaatg gtgttaaatg tgtttgatgg cacagccacc
    34621 attaccaatt taaccatgcg aaacccgctt ggcatcgcac caagactcaa tgcggacatt
    34681 gcgctacggc atttggactt aggcaactta acacgcacat tcagctttgg tgcgattgaa
    34741 ggcaagctgg atggtgatat tgacgacttg cagttgcaaa actggaagcc tgtcaaattt
    34801 gatgcggaag tgcatagcag cccgggcaag tatgctaaaa aaatctcgca acgggcggta
    34861 gaaaatattt cagcgcttgg tggagcgggt gcagcagcgg ccatacaacg tagttttttg
    34921 caatttttta agcagtttaa ttacgaaaaa ataggcttaa gttgcaaatt acgcaacgat
    34981 atctgtgaaa tgaatggcat tgcgtcaacg gatggcgggt atatcatcgt taaaggcagt
    35041 ggcatccctg cgattaccgt gttagggtac aatcattcag tgggttgggg cgagttgttg
    35101 gcacgggtaa aacgtgtgat tgacggcaac acaaaggcag ttgttaagta attgaattta
    35161 aaaaataaat gcttagtaaa acttaaggat aacaaatgaa aaattggttg atggtaataa
    35221 gcatagcggc aatgttgcct gcttgcgtaa caattaatat ttactttccg gccgcagcgg
    35281 ccgaaaaagc cgcagacaaa atcattgatg acgtttggca actcaaaaat ggtgaaaaac
    35341 caactgtgaa aacgattgaa cctagtgcag aaccagcgcc tgctgactca gccaagtaat
    35401 tcacctaaca taaggataaa tcatgaaaaa aattatattg agtttagtaa tggtattgag
    35461 cgttttattg gcgagccagt ttgcagtagc tgcggcggat ttagaagtga acacacccgc
    35521 catcactgcg attaaaagca gcatgcaagc acgtcatggg cagttggcag gcttttacgc
    35581 gagcggtgcg attggtttaa caaaggatgg cctaattgca gtgaaagatg ccactgccgt
    35641 gccgctcaaa gaccgtggcg ctttaaacgg cttggtgtcc gcagaaaatg cagaccgtaa
    35701 aaatttatat aaagaaatcg ccaccgccaa cggccaccca gagtggcaag gtgagattca
    35761 aagcaccttt gccggccgct ggatagacaa agcgcaaagc ggttggtttt atcaaggtgc
    35821 tggtggttgg gcgcaaaaat aactttttga acaaaggctg cttcggtggc ctttgttttt
    35881 tgtaacagtt tttttgtagt atgctcttaa ctatgacacc caccctcgta ttcgacattg
    35941 aaaccattcc cgataccgca ggcctacgca cgctactaga tgtgcctgcg cacgccactg
    36001 atgaagacat tgctaatatc gcttttcatc accgccgcca gcaaaatggt agtgagtttt
    36061 taccgctaca tcagcataaa atttgcgcca tttcatgtgc gttgcgtgag ggtaaccatt
    36121 ttaaagtgtg gacattgggt gatgccaact ctacggaagc tgagattatt cagcgctttt
    36181 ttgatgggat tgaaaaatac acgccgcaga ttatcagctg gaatggcggt ggttttgatt
    36241 tgccggtgct gcattatcgc gccatgatta acaaggtaca agcgccgcgc tattgggatt
    36301 tgggcgagga tgacaaagaa tttaagtgga acaattacat tagtcgctac cacacgcgcc
    36361 atttggattt aatggatttg ctcgccatgt ttaacggccg tgccaatgcg ccgttggatg
    36421 aaattgccca actatgcggt tttccgggaa aattgggtat ggatggcagc aaggtgtgga
    36481 acgcatacct tgccggtgaa attgatgcaa ttcgcaatta ttgcgaaacc gatgtggcca
    36541 acacgcattt ggtgtatttg cgttttcagt taatgcgcgg gcacctcaca cccgtcgcgt
    36601 atgaggcaga agttcactta gtgcgcgata cgcttttaag ttattcagca ccgcattggg
    36661 cagaattttt ggccgcgtgg aagccgtaaa tattttacta aacagcaggc aaaaaaatac
    36721 ttttttaagc ttattttagc aacaaccttt ttcgatgctc tggaagcctt ataaataaag
    36781 gcttgcagag cataaaaatt ttctcgagcg tttttacgcg tgtttgcgca tatgttttgt
    36841 atgctaattt aacccatgat tcactgcatt ttgcgttaaa atctcgcttc aaatttcacc
    36901 acaggttttg tcatggctcg tcgctccaga agtcgcaatc aagcgcccat tattccagaa
    36961 atcaagtttg ccacgattga gtcgcttgac caagaaggcc gtggcgtggc gcacgtagaa
    37021 ggtaaaaccg tttttattga tggtgcgctg ccgcaagaga aagtcagcta ccaatcccat
    37081 catatcaaag ccagttatga aattgccaat gtggtgcaag tgctgaagca atcaaatcag
    37141 cgtgttacgc ccaaatgccc acattttggc ttgtgtggtg gctgtaagtt gcagcatctg
    37201 gattttgccg cgcaagtggc agccaagcaa cggctgctgg aaaatgattt aaaacatatc
    37261 ggtaaagtaa agcccgacaa tatattaccg ccgctctttg gcccaacttg gggttaccgt
    37321 cacaaagcgc gcttgagcgt gaaatatgtg gagaaaaaac agcgcgtatt ggtgggtttt
    37381 aatgaaaaag ccacgcgtta tgtagccgat atgaactcgt gcgaggtgtt ggtgcctgag
    37441 gtgtcgcagt taatagtgcc attgcagaat ttaattgtgc aactcagcat ccgcgataaa
    37501 ctgccgcaaa ttgaattggc ggttggtgaa aatcctgcaa acgtgcgtga aagtgcccac
    37561 gtgatagtgt tgattttgcg cattatggat gcgctcaatg cgaatgacga agatttgttg
    37621 aaaagttttg cagatgaaca ccacgtacaa atttggacgc agaccaaagg cccggatacg
    37681 attaagccgt tttatccatt gagtgcagcg caattacaat atagcctgcc ggagtttggc
    37741 ctgacttatc cgttcaagcc caatgaattt acccaagtaa acccgcaaat taaccaagtg
    37801 atgatacgtc gcgctatgca attactggcg ccaaaagcgg gcgaaaatat tgctgatttc
    37861 ttttgtggca tcggtaattt tactttgccg attgccagaa gcggcgcgca agtactgggt
    37921 ttggaaggtt tggctaattt ggttgaacgt gccaatgaaa gcgctgcgtt gaatcaaatc
    37981 agcaacacgc aatttggcgt ggccgattta tttaaaatga cgcctgaggc actcacgcaa
    38041 ttaggccgtt ttgataaatg gctggtggac ccgccgcgcg atggtgcgat tgagctggta
    38101 aaagcgttgg atgctaacaa tcgcccacaa aaaatcgtct atgtgtcatg caacccagcc
    38161 acgctggcgc gtgatgctgg tatgctggtg aacgaaaaag gttatacgct catagcggcg
    38221 ggcgtgatta atatgttccc gcatacggcg catgtggaat ctatcgcgct gtttgagctt
    38281 acaggaaaaa cagaataagt gtctatgcgg gcgaattaca gcgttgcgcg gtactcgcaa
    38341 cctcgctgta cattatgtac tatctcggat gctccgttcc gtgcgccttg tacttcatcc
    38401 cgctcgacga cttattctat gtttccttaa tttaaaaacc agttaagtgt tactgtttta
    38461 catgaaacgg tcatcgtttc gtcacaattg gcgcgtagta tcaatggcat ccattgataa
    38521 aaaaatccta tgccttcaac ttttccgcct ttggaactac tcaatcgtga caccagcatt
    38581 ctggcgttta acgagcgcgt tctaagcctt gcacaaaatg caaattatcc gctgttagaa
    38641 cgtttacgtt ttctttgcat tgtttcttcc aatttggatg agttttttga agtgcgtgct
    38701 gccccgcatg ttgaagccat gcgtgatgga attcgccaaa gtgacattaa cgtgcaaagt
    38761 tatcaggcaa ttgctgaaga tgcacatctg ttagtcgaga aacaatatca aatatttaac
    38821 ggtgagttaa tgccaacgct cgccaaacaa ggtgtgcatt tggtgtcgca tggcgaccgc
    38881 acggcggcgc agcgccgttg ggtttcaaga tactttgaat ctgatgtgcg tccattattg
    38941 gtgcctgttg cgcttgatcc atcgcatccg tttccgcagg tggccaataa atcactgaat
    39001 tttattgttg cactcagtgg tcaggatgcc tttggccgca gcaacaatat tgcgattgtg
    39061 cgcgtgccta gagcattgcc acggctgatt aaactgccaa aaaatctcag tgataaacag
    39121 caaaaatttg tttcgttatc gagtgtgatc cgtgcgcact tggccagttt atttgatggt
    39181 cgcgaagtgc tgaatttctc acaatttaga gtcacacggc attcagattt agcggtggac
    39241 gaagaagatg ttaaaaacct gcgcactgct ttacgtaaag gcttggtgca acgaaatttt
    39301 ggcgatgcgg tacggcttga agtatcgcat gcctgcgatg aaaaactggc caagttttta
    39361 ttacagcagt ttgatttgcc caaacaatca ctctaccgcg tgaatggttt ggtgaatttg
    39421 gctagactca tgcaattggc cgatttagca gaaggaagta acttaaaatt taagccattt
    39481 gagccaaaaa taagtcagca attggtacca aaccaatcca tttttggcca gcttaaacaa
    39541 cgcgacattt taattcatca gccgtatgaa agctttgagt cggtgattga ttttttgcgt
    39601 gaagcggtga atgaccctga cgttgtatca attcagcaaa cgatttatcg cactggctca
    39661 gacccacgca tgctcaaact attacaagag gcagtgcgcc gtggcaaaga agtgatggcc
    39721 gtggttgagc ttaaagcaag gtttgatgaa gaagcgaata tcaactgggc tgaagcctta
    39781 gaatccgtag gcgcgcaggt ggtgtatggc attgtgggct taaaaacgca tgccaaaatg
    39841 ttgttggtga tgcgtcgtga aggtgttggt ttaaatggaa gtaccaagct tacacattac
    39901 gggcatgtgt ctacgggcaa ctataacccc cgcaccgcaa agctctatac tgatgtcagc
    39961 atgcttacgg cttgtccaaa aatcacccgt gatatggagt atttgttcag gcatattgcc
    40021 agccaggtaa agctgccgcg cttacaaaag ctgctggtgg cgccatttaa tttgcatcgg
    40081 cagatgctta gtaaaattaa aagtgcagag ttggcggcaa aagcgggtaa acaagcgcat
    40141 atcattttaa aaatgaacgc attgacggac gaagtgttgg tggtcgcgtt ggtgaaagcg
    40201 ggtcgtgctg gcgtgaatat tgatttgatc atacgtggtg cctgtatttt gccagttgat
    40261 gcaccagggc tggaaggacg cattcgcgta cgttcgatta ttggtcgact gttggaacac
    40321 tctcgcgtat tttattttaa cgtgcaggat gttgagcaaa tgtggctttc aagtgctgat
    40381 tggatgaacc gcaacatgat gcgacgtgtc gaaattgcct ggccgattgt agacgctgaa
    40441 aatcgcgcgc ggattttgca agaatgctgt caattaagtt tggatgacac gcaagatgcg
    40501 tggcaattgt tagcgggtgg ggtgtatcag ccagtagcaa gtttggcgcg aaaaaatcac
    40561 gagcatgcgt ttagtgcgca acgcacgttg attcaacaat atggagaata atttttagtg
    40621 agtaaacatg gaaaatttaa tatgttggcg acatgccgaa gctgaagcag atagcaaaac
    40681 tggcgcagat attgaccgtg agctgacaaa acagggccgt aaagatgccg ccgccatggc
    40741 gaaatggttg catcaacatt tgcccgccaa tactgaagtg ctttgcagcc cggcgcgtag
    40801 atgcttagaa acggcagctg cattacaacg gtttaaccgt caacaaaaag taacgattgc
    40861 cgaatatttg ggcgttgata atacggtgga taagattact aagcatgtgc tgaatgaaga
    40921 tagcagcaga accattttaa tcatcggcca tcaaccgaat cttggcctat tgatagctcg
    40981 gttgcttaaa atggagaatg aagcctgtgt ggtgaaaaag ggtgctgtgt ggtggctacg
    41041 tcagcgtcat gttgatgaca ccgtgcaaac ttatttattc accgtacagc acccggattt
    41101 tgtttaagat aaaacggatg agttgatttt ttaaagcatt tattttttct taggtctgcc
    41161 cgttttaatg gtaggcactt tgcttaacgc ggttttaagg gccgcatcac ttagcgctgt
    41221 gagttgcata atacccgcac gtaataattc acttttcttg gcaggtgcgc ccagctttgc
    41281 caagcgctct tttaatacat aaagttgcgc atattcagct ttaggaatgg taaagctgtc
    41341 gcgctccatt ttaggtttag gaattttttc tttcacaggc ttggctgctt ttggtgcttt
    41401 tttagcagct gatttcgcca caggcgttgt ggttgctggt ttgacaggct tttccttaat
    41461 tgctattgtt gattttgaag ctggctttgc agtgacaggc ttggcagtta ctggttttgt
    41521 tgatgctgtt ttagcaacag ttgttttgag cgctgccgct tgaatggcat tgatttttga
    41581 catgaaaacc tcctaattaa acgatataaa cagtttatac cgtttaatta ggtttgagta
    41641 tgaaatattt atgacaattt acgagttagc gctaatgtca aaacgccagt taatttttaa
    41701 ccacgccaag cgttcttctt caagcaagta ataggattgc ggaaatttat ctggccatga
    41761 tggtggcaaa cttaatacga tatggccgtt taaaaagcgc agttgaaaac cgcgtaagtt
    41821 cggttttttt cttgcgtgac acaatataat gctcaggcgt aagcacacta attgcatgac
    41881 aaaaatacgg tctgaaaagt ctgcttccaa gcgttttaat ttttcttttt ggcctaaaat
    41941 cagcaggctc aggcaatgca attcatgttg cgaaaaccct gctggttcgg tgtgctcaag
    42001 aatataagcg ccatgcaaat gcgcgtctat cggtgaaaca atgctgccga tttcatgcaa
    42061 caacgccacc caatgcagtt tacgcgcatg cgcctgctgt tctccagatt caaactgaat
    42121 atctgcgctg attttatcaa ataaggtatc ggcaacctcc gccaccgtat tggcatgcgt
    42181 ttcatccaca tcaaatttgc gcgccaagcg cagcaccgag gcattgcgga tatcaagcat
    42241 ggcattttcc cgcgcaacca tgtcgtagag taggccgtgc ctaagcgcac ctttggccac
    42301 gtgtaaggtt tcaatttgaa aataatcaaa gatgccgatg agtattgcca atccacctgc
    42361 gataacgggt ttgcggtctt ctttaagtcc aagcaatttt aatttttcaa catgtttggt
    42421 gttcagtaat tcttcacgta accaatgcaa accttcttta gtcaccgtat cgatcggcct
    42481 gccgtattgc actaacacat ctgccacggc gccgacggtg cctgacgcgc cgtaagccac
    42541 gtcccactgt tgcgggttaa agtgtgcgcc tagcgtttca aaaatggatt cagcggcaat
    42601 ctcggcgcgg gtaaaagctt tttctgtgaa ttctccactg gcaaaatgct tcagtgacca
    42661 tgccaccgaa cccacatgaa gcgatgccgt gtggtgcgcg gtaaagcctt ggcctaaaat
    42721 aaattcagtc gagcgcccgc caatgtcaat caccaaacgc ttttcatttg actgcggtaa
    42781 aaagcggctg acgccttggt aaatcaggcg cgcttcttct acgccagaaa tcacttcaac
    42841 atcaaacccc agcgtttttt tagcgagttt ggtaaacact tcgcggttgt tggcctcgcg
    42901 caaggtttgc gtggccaccg cgcgcacatg tgcggcatca aagcctttta agcgttcgcc
    42961 aaatctggcc aggcatttca ggccgcgctc aatggcttca ggcgtcaggt tgcggtcttc
    43021 gtctaaaccg ccaccttggc gcacggcttc ttttaagtat tcaacacgtt caatatggcc
    43081 gctatcgagc cggccgattt ccagccgaaa gctatttgag cccaagtcga tggctgcgag
    43141 cagacttttg tctgcgggaa taggtgtgtg gttttccata atgggcaatc tgtgcgtaaa
    43201 gtaaaacaat aatattgttt atattgccat tatagtttga aattttagtg acaattgaaa
    43261 ataaaactga gagatgtcag actgaatgtt aagcgcaatt gtgttgaggt gaacaccttg
    43321 aaaaaatgct aaccttgccc tgattttaca tacaaccttt tgcactgccc tgtgagcctt
    43381 atcaataaag gcttgcagag cagtaaaatt ctctcggcgt atttaaagca aaaatttata
    43441 ttgttttggt gcttgaaaaa cgcgcgtttt atccccattc aaatcttaat tgttttaatc
    43501 actttatttt aggagcttaa tttatgtccg cagagactgc acaatctgaa attcaaccaa
    43561 aacaatcgat ggcttttcaa gctgaggtta aacaattact tcaattgatg attcactcgt
    43621 tgtacagcaa caaagaaatt gtgttgcgcg aattgatttc taacgcctct gacgctgcag
    43681 ataaattacg ctttgaagcc atcagtaatg gtgcgctgta tgaaaatgac agcgaattga
    43741 aaatccgcgt ggcgtttgat aaaaccgcac gcaccgtgac catttcagat aatggcattg
    43801 gcatgagccg cgatgaagtg attgccaata tcggcaccat tgcaaaatca ggcacaaaag
    43861 aattttttaa cgcgttgtct ggtgaccaag ccaaagatgc gaatttgatt ggccaattcg
    43921 gcgtaggctt ttattccgcg tttattattg ccgataaagt gagccttacc acacgccgtg
    43981 cgggtagttc agaagcagtg cgttgggagt cgagcggtga gggcgacttt accattgagc
    44041 cagcagaaaa agccagccgt ggtaccgacg ttgtgctgca cttgcgtgaa aatgaggatg
    44101 aatttttaag cgattggaaa ttaaaaacta tcatccgcaa atattctgac cacattacct
    44161 tgccgattgt gatgaaaaaa tcggagtggc gaaatgacga agtctcggca gaagataaag
    44221 caagcgcaga agtgcccacc gatgaagatg aaactgtcaa taaagcctcg gccttatggg
    44281 cgagagccaa aaacgatatt actgaagaag agtatcaaga gttttacaaa cacgtttcgc
    44341 acgattttga aaacccactg gcttatacgc acagccgcgt tgaaggcaag caggaataca
    44401 tttcgctgtt atatattcca ggcaaagcgc cgtttgattt gtatgaccgc gatcgccgcc
    44461 atggcattaa actgtacgtg aaacgcgtgt ttattatgga agatgccgaa aaactcatgc
    44521 cgcaatatct gcgctttgtg cgcggcgtga tagatacgtc tgacttgccg ttgaacgttt
    44581 cgcgcgagat tttgcaatcg agccgcgatg tggatgcgat taaagccggc tctgtgaaaa
    44641 aagtactggg gttacttgaa gatttagccg aaaacaaacc tgaagattac gcaaaattct
    44701 atgcggaatt tggccgagtg ctgaaagaag gcccgggcga agattttgcc aataaagaca
    44761 aaatcgctgg cttgctgcgc tttgcttcca ccaaggccga tactgatgtg caggatgttt
    44821 cactcaaaga atacatcgcg cgcatgcagc ctgaacaaga gctgatttac tacatcacgg
    44881 cagatagttt tgccgctgca caacacagcc cgcacttaga gattttccgc aaaaaaggca
    44941 tagaagtgtt gttgatgagt gaccgtgttg acgaatggct actcggcagc ctgacagaat
    45001 ttgaaggcaa gaaattgcag tcaatcgcca aaggtgactt ggatttaggt aagctcgaat
    45061 cagacaccga aaaagagatt caaaagaaaa tcgaagaaga ggcaaaatca ctggttgaaa
    45121 aaatcaaaca ggcattgggc gagcaagtga aagacgtgcg cgtgacgcac cgtctgactg
    45181 actcacctgc gtgtttggtg agtgatgcac atgatttatc gggcaatttg gcacgtatgc
    45241 ttaaagccgc cggccaaaaa gcgccagaat ctaaaccgat attagaaatc aacccaacgc
    45301 ataaattggt gaaacgttta gaaggtgaaa cggccgaagc tttgtttggt gatttggcgc
    45361 atgtgctgtt tgaccaagcc ttgctggcag aaggcggcca gttggatgac cccgccagct
    45421 ttgtgaagcg gatgaacagc ttgattagct aatgcaaatt tgcttgtaga ccaatagggc
    45481 gaaatgctaa atttcgccct atttttttgt gcaaaaatag attaaaaatc tagcaattaa
    45541 aattggcata aaagccctta aaaaccttcg agaaaaaatt aatgctctgt aagcctttat
    45601 ttgcaaggcc tacagagcat caaaaaaggt tgctaacaaa aattagcgtt tatgccagta
    45661 ttatttaatg cacatttcac gcaaaattta atccttgcga tagaatgaaa ctcgctaggc
    45721 aaagcgagcc aacctaaccc taaaaagcga cttaacaatg accatgttaa acatacaacg
    45781 cgcaagtatt gtctacggta ttatattgca gtttttaacg cctggccggt ttatcaaatc
    45841 gctaggcttt ttgatgttgg caggtatgcc gttaatggcg cattcagaag gttacgatag
    45901 gctgccggag gcgatacata tcgtttttgt gagcatggtt tatgtttttg caagtcgggc
    45961 tgtttattgg tttgttggcg ccgccatttt tgcttgggtg cttagttttt tgctaaggct
    46021 atacactaag tcagataacg cttataaaaa ttcatcagct gagcaacatc agccagtaaa
    46081 taatgtggtt ggcctgcttg ttatggttgg ttgggggcta atgtttttgg ctgtgttggc
    46141 cttgattgtg gttggaattg gcagaagtat ggggtttaag gacgtttcaa cattgcctaa
    46201 aactcgctac cagcctattc aaatcagtaa agaagagcgc gagcggcagg caagtcttat
    46261 tcctaaagtg gttgacgaca tgtcatggaa aatttattca cccttgaata agccatggcc
    46321 ggctaatgaa ggggcacttg aaaatgaaag gcaagagggc gcgcgcggtg gtaaatatga
    46381 tgtggccatt tataaaacta gcacatcggg ctcaatattt tttaagttat gttacgtggt
    46441 aacaggccgc tgcgatggct tacgccatgc ctatatgctt aatcaggcgc gttatacttt
    46501 tcaaaatgta gaagatggcg aatatgaagt acgtatgctg aacttaaaaa caggtagcgc
    46561 atggcgaagc aaacccttta ctgtgccacg ttcatctcct gtaaaagtag ttgccgcttt
    46621 tcaattaggc gatggtatta aagaaggcac acccgtcgcg ccagaggatt tttgatttca
    46681 cttgcagccg cctagttgcg tttaggcaac tttatatcct tgcgtagcat cgtattttcg
    46741 gtcgcaacca atcaggccat ttataggtgg cccgtttttt gctagaatag cacttgaaaa
    46801 tcaactttta atttgcatct caaggccatg aatgaaaact ccagcaaaaa catctgataa
    46861 agccgttgct aaaaccaaaa cggcaactaa agcaaaaacc gataccggcg cggcagctaa
    46921 accaaaaaaa actgccaagg caaagcgcgt attgcctaag ctctcgccag tagaccaagc
    46981 gttacagaac catttaattt tttctagctt taaaaccaac gatgcggcta ccccgcgcga
    47041 ttggtacgat gcggccagct atacggtgcg cgaccacgtg gtagcgcgct ggattaaaac
    47101 tgcggaagcc tattacgaaa aagacccaaa acgtgtgtat tacctctcat tagagttttt
    47161 aatggggcgt atgttgagca atgctgcact gaatttgggc attcaagatg aactcaaaga
    47221 cggcttggca agcttaggcc gcgagctgga aaataccgta gagatggaga ccgatgcggc
    47281 actgggcaat ggtggcttag gtcgtttggc agcttgtttt gttgattcca tggcaacgat
    47341 ggatattcct gcgacaggct acggc
//
LOCUS       DAOQSI010000003        35085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_649_length_35085_cov_5.535370, whole genome shotgun sequence.
ACCESSION   DAOQSI010000003 DAOQSI010000000
VERSION     DAOQSI010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 35085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35085
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_649_length_35085_cov_5.535370"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..416
                     /locus_tag="PKL42_00450"
     CDS             111..416
                     /locus_tag="PKL42_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HNU65564.1"
                     /translation="MKIIQTIPDAWQNIAQLFIALGDAHRQRILLTFEKGERMNIMQI
                     AAASTLSRTAVTHHLKILRESGALQSEKIGKEVYFWVDKGFMTNAISNVLHYIETEI"
     gene            458..2590
                     /gene="aas"
                     /locus_tag="PKL42_00455"
     CDS             458..2590
                     /gene="aas"
                     /locus_tag="PKL42_00455"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HNU65565.1"
                     /translation="MLAKLLRFICKILFRVEVRGLENVPKEDGLLIVANHESFLDGLL
                     LGLFLPKKATFVVHTSVLKKWSFRQALRLTSYLAVDPTSPLAMKKVIKLLEAGENVVI
                     FPEGRITLTGNLMKVYDGPGFVAGKTAADILPVRIDGAAESYFGRLTRHHPRRLLPKV
                     TLTIMPTTSIAMPKATHHGFPTGKQRRRIAGEGMRSVMQKMLFQSQKSRTLFEAFLDA
                     KDKFGDNYKLIEDINETEETYQNVLKKSLALGRIVTKVSRAGETVGVMMPNITNTVAL
                     YLGMSAFNRVPAMLNYTSGTAGMQNACIAANIKTIITSHKFIETAKLESVVAGLTNLN
                     ILYLEDLRAQFGVVDRAWLIGYALHYPRAAMEVSKPDEPAVVLFTSGSEGKPKGVVHS
                     HKSILTNISQIMAILDFNVTDKWMLVLPVFHAFGFTASLLTIYNGMKTVIFPSPLQYK
                     VIPEVIYDKGCTVFIATSTFLANYAKYAHPYDFYKLRVVIAGAEKLNEEVRRTYQEKF
                     GIRILEGYGATECAPVLSANTAMANKNGSVGQFVPGLEHRLETVTGIESGGLLHVHGE
                     NVMMGYYLFDNPGVLHPPSEGWYSTGDIVELDAQGYIHIKGRVKRFAKVAGEMVSLEV
                     VEHIANTAAPEHQHAASTQEDAGRGENIILFTTDPELKREDLQIVAKNTGSPEIAIAR
                     KIVRVEEIPVLGTGKTDYVTLKQMAETA"
     gene            2587..3627
                     /locus_tag="PKL42_00460"
     CDS             2587..3627
                     /locus_tag="PKL42_00460"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNU65566.1"
                     /translation="MRKALLITLLSLLLVPVAIGAWVKQPFNHEIGELKFAGLAEADP
                     ERLKADVEKLSVTYATRHFGDVRTLDLAANYIQAEFKKTTERVEIQSYQVDSEKFHNI
                     IAHFGPKIDYTATYENGPPKPIIIGAHYDVHEDTMGADDNASGVAGLLELARIFAKKP
                     PAIPVQIVAYTLEEPPNFRTDSMGSRQHVASLMQAGIQPELVMVLEMIGYYNEAENSQ
                     EYPVSLLKWFYPTRGDFIGVVGRFSAAPTNKMVKMVMQSAHPEFPVESISAPAMMTGI
                     DFSDHASYWLHDMPAVMITDTSFYRNPNYHKATDTADTLDYTRMAKVVEGVHTVTVYM
                     SDSKNIKDKSDE"
     gene            3620..5206
                     /locus_tag="PKL42_00465"
     CDS             3620..5206
                     /locus_tag="PKL42_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HNU65567.1"
                     /translation="MNNFISAKQIIAFCIWCFTSAPFIAYGNNTENAQNNAEVLTLNS
                     PQAVTGKYETDAQAVLAYIASSQQLSAQLPSFMVESNQAIKKHKGALPSAYALRLAKA
                     LSEAHDLRDGLFKQALTHRSALYRVDDDISDNERVEEIVIAMSAAVTLFQNSKTMHQA
                     FDKNPLLKNKLNEGYPEFGIPEGFYDSSTMRSNNTEYRKAFTDAVRYFADNREAIEAE
                     INRSSTSIQSLYQHIAKSPMLTGFKGANVFKRIAILPFKAANGVVDVSGRSLNHAKFT
                     SSKIVGNTMGAVRWRAGKLKDDAQVFQTMLSQLQPGDILLEKTPFTLTDKSIPGHFGH
                     VAVYVGSTAQLKELGLLEQAIVQKNLAKIEQGHSVVEALRSGVQLNKLEAFMNVDDVA
                     VLRPKNLSIEDQRQAVTLALGNLGKKYDFNFDVNTTDKIVCSELVYIVYPQVDFETKN
                     VLGSFSITPDDIAQRAGSNEADPLEVVLFAHDGKLVCEQQSPNKHEGIVLYERLVKSA
                     EKTNANNASGVQRAAFSGFL"
     gene            5229..6443
                     /locus_tag="PKL42_00470"
     CDS             5229..6443
                     /locus_tag="PKL42_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNU65568.1"
                     /translation="MTKLTNYQSTSAFSNAMVGIKAWLETGHRNPNLTGLPESLKTHP
                     LMHQVWDGIDATQVWDCHAHIIGSGDSGTSGAWATPRMDTWRHPILKIQKWFHVNGAG
                     LDRKNEDTSFVSRLAHLSSEMPEGYKTMLFAFEWAHDETGKQIKEDSIFHIPNDYAAR
                     IVAQHPQHFEWVASIHPYRADAIDALEQAHAQGAVAMKWLASSMNIDPASSKCTKFYQ
                     KLQQLNMPMITHVGRESAVPGGDLNVNNPLKARAMLDAGVRVVMAHCASDGVDKDYDH
                     GNKLVSSFELFTRMMDKPEYEKLLFSDISATTLFNHAWVLKHVLQRTDWHHRLHNGTD
                     YPLPGVFPLISSEQFYREGMLAKEHVPFLQEVRKYNPIMFDFAMKRLLSYKEKQFPTS
                     VFETRGFFRQLI"
     gene            6454..6876
                     /locus_tag="PKL42_00475"
     CDS             6454..6876
                     /locus_tag="PKL42_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65569.1"
                     /translation="MKQLLMATLLATAIVSPLSALANGSAASLDASKTLSVGVGSIVV
                     GSASLVAASGQIVVESVENTADGVLMVLHGVGAGVSEAGKFSLKITGDVSGAASVAVG
                     HSVEVVAETAGSAIIASGKVIAFIPNALGKSLMHHSEL"
     gene            6923..7717
                     /locus_tag="PKL42_00480"
     CDS             6923..7717
                     /locus_tag="PKL42_00480"
                     /inference="COORDINATES: protein motif:HMM:NF021437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2145 domain-containing protein"
                     /protein_id="HNU65570.1"
                     /translation="MMKKQLLSLLLCLMLHPVFAEAGTACTEKPQSAETVQKAFQLAI
                     KTREALDASGAEVALVGRVGQDLSKYNLRYSHMAFVWRDHPQGRWLAVHELNQCGTAN
                     SALFNEGLANFFFDDMFAWDALIVTPSPNMQAKIVALLKNTAYLKHVHEPHYNMVAHP
                     FSTKYQNSNQWVLEILSSAMSEELFTSRSATQTWLKQANFKPSVLRIPTLKRLGGRMF
                     QANVAFDDHPNADRFAGNIAVVTVESVKEFVKQQDSAVQELTVSLQ"
     gene            7735..10512
                     /locus_tag="PKL42_00485"
     CDS             7735..10512
                     /locus_tag="PKL42_00485"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HNU65571.1"
                     /translation="MELALLGLIGVALGVIILVGSIAGFFGMFSSSDKAKKLYQLEQE
                     TEKLRKEMARISAQIDALKANPPQTTPVQTAALKVADTPTVSVSEPIVASVLEKPMAE
                     SAISAVVQPTVKLEPTTRTIEKTIEKPIEKPAYVKPAYTPPPPNFIERGFAAAKEWLF
                     GGNTMVRAGIVILFIGISFLLKLAVDNGMIPIEYRLLAVAMGGIALLIVGWRLRDTRS
                     QYAWALQGGGVGILYLTIFAAMKMYGLIPAGAAFGLLAAIAFLSAFISVKQSALPLAI
                     LGFAGGFLAPILTSTGSGNHIGLFSYYLVLNLGIAYVALYKSWRSLNVLGFAFTFIIG
                     SIWGAKSYTPDLFATTEPFLIAHFLLFTVIAILFAHRQSTKASDYVDATLVFGTPLVA
                     FSLQYALLKGTPFGLAYSALALGIFYLGLAWWVLNRQRETLKFLGECFLALGIGFVTL
                     TLPLALDGRWTSAAWAVEGVGLLWVGLKQNRKFPAFTGLSLQLLAALAFAKGWGLTGY
                     GGFGHQNLFLGVGFIALSGWACGALLNAYRPNKFDWMRTPLAVWGWLWWVGAGLTAIS
                     EFMPRDNFMHAGLIFVALTSALLPFVANKLKWSRLATLSTLLLPAMGIALCWEAILTL
                     FSYSSYSILKNNGFIAWPLAIGAYAWLISRNAIPEKAWLRAPLLWVMAAVGVLEWHHQ
                     LGQYVSESHVWAQIGWGVIPMLIIGAVTYWQFAKNQLNATRLDSARTWAWVGSLPLMA
                     LTVVWYFNMSLNASGNAAPLPYFPIVNPLDITLAGALLLLLIWQRAVSKHLGKLQHIN
                     PVLASLMGFALLNGMLLRTLHHWIGTPHLWVSIFNNSTVQMAFTFLWGITAFGLMLLA
                     HKKERRSLWIVGASLMGLVVLKLFLLDLSQSGSVDRIISFIGAGMMLLVMGYFAPLPP
                     AIKANELKESA"
     gene            10509..11864
                     /locus_tag="PKL42_00490"
     CDS             10509..11864
                     /locus_tag="PKL42_00490"
                     /inference="COORDINATES: protein motif:HMM:NF024562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 domain-containing protein"
                     /protein_id="HNU65572.1"
                     /translation="MKSFKKIIATAALCFSGLVSAESFNLNTTSSEPLYQSLLTKEVY
                     AASHQDHLQDLTIINASGEQVPYALLHYYDLHPQTATSIKRQPLSVFPVQESSLNNPN
                     ALTIQLENSAESSNVSLNINNATADAKTVFLVDAGKKHPPLQTLSFDWQGSEGVLLSL
                     EVLASDDLKNWSSVGHGVLLKKTAEGNAILQNSITLDYPTEARYLQVRSTKNEALQLS
                     RVDAAYNNVRALTPAIMWQTVQFVQREQDNKNGVVNLDFEALGRYPASFVQVKLPQDN
                     TITSASIFVRNKQDAPWQYIATTSLYQMNQANKTYTNPDILINSTTARFWRLAFNQAN
                     GGIGQQNPQLNLGWLPQTVVWNARGQAPFKLEVGEKPSVVNTVSVANLIPDFKIEKVH
                     QLPVANISAPVASAVAPQPKNTWTAAPDYKSWMLWGGLLLGVMLLAGMAVSLLKSDNK
                     P"
     gene            11896..13245
                     /locus_tag="PKL42_00495"
     CDS             11896..13245
                     /locus_tag="PKL42_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNU65573.1"
                     /translation="MSNQFQLLREQRFRPFFFTQFLGAFNDNVFKTALITLVAFHAAS
                     LTTIDGGTLATVLPGLFILPFFLFSATAGQLADKFEKSQIIRYVKVFEIAIMLFASLG
                     FYFHHIGLLAAALFLMGTHSTIFGPVKYSYLPQHLKQNELVGGNGMLEMGTFVAILLG
                     QVLGAWVAMQPQHEIITSLVILLIAVMGYATSRGVPKSPPAAPHLKINWNPVTETYRN
                     IQFVWQHQQIWLAIVAISWFWFFGATLLAQFPNFAKVVLYGDESVFILLLSVFSIGIG
                     IGSLMCEKLSKGKVELGLVLFGAIGLTVFGADLYSSSTWIHAHWNNKTLFDFASFISR
                     HHDMHGQVIFAHWRLLADIALIGFFGGLFIVPLYALIQTRAEKSHQSRVIAANNIINA
                     FFMVASAGFSILIFHNELTIPELFLVTALLNVLVMIYLCFRQPEYFKAFIRWISLGK"
     gene            13507..15474
                     /locus_tag="PKL42_00500"
     CDS             13507..15474
                     /locus_tag="PKL42_00500"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HNU65574.1"
                     /translation="MQLIHRIKQSIWQLANKCGAFAPLFAMLLLGLILLSLSRVGLVL
                     WKLDRVLATDKLVGIFLQGIRVDIIQLSLMALIPLLLAPILATKQLFKVWQKFTYIWV
                     TISILLLVFLEIATPGFIMEYDVRPNRIFVEYLKYPNEVMSMLWTGFKVHIFATLGFS
                     LITLWLMRRFMKPWLAAKPTWSNKKLWLIWPLIFLLSAFAIRSSLGHRPANPALFAIT
                     QDSMVNSLILNSGYSVIYAVYSLQHEAKSSKIYGKMAKEDIFKITGAKDTDLPTLTTL
                     NPSAKRDKPLNLVIILQESLGATFVESLGGVPVTPNLEKLKEQGIWFEQLYATGTRSV
                     RGIEAVVTGFQPTPADSTVKLSLSQKNFFSLAGLLSKHGYSTEFIYGGESHFDNMRGF
                     FMGNGFQSIIDQKDYKNPVMLSSWGVSDEDLLNKTHEQLLAHHASGKPFFTLAFSSSN
                     HAPFEFPDGRIELYEQPKNTDNNAVKYADYTIGEFIKKAKASPYWKDTVFLIVADHDI
                     RVRGDTLVPIERFHIPGLILGADIAPQKITAMASQIDLPVTMLSLMGLQTQHPMTGHD
                     LSQIPADYLGRAMMQYNYNFAWMEQTPTGNQVVVLRENKAPAHGVYDTKVKRLHEVSP
                     PQNAITLEQRALANTLLPALLYSEQKYRVPE"
     gene            15471..17723
                     /locus_tag="PKL42_00505"
     CDS             15471..17723
                     /locus_tag="PKL42_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HNU65575.1"
                     /translation="MTKIAEKKQPSMLTKQVIFDGLRYILTGGLRIKKGHLPVQAHQI
                     PHDFIGICVASNQDAAVDDYVVANIKLLGIQRVRLDFTYTDVDNYQVRFLNRLIAEGF
                     DVTLHLLQPFEDAKNMQSVEAQLKWRKFLVTVLDTFGQKIKAVEFGNTLNRKRWAGYD
                     WSGFMQAWRIVHQEITSRNITLIGPNVQDFEPLYNVSLLKTFKQQKLLPDIHSNNLFV
                     ERVTEPERFDHRILKYRWATLLKYNLVKKARILQKIGQDFGVPQTVSSVAFWAIYRIQ
                     RFLPDGEQKQADYAARYFLLLAASGALASANWGALICQREGVIDDGLTEQEYPDLERV
                     AHYQQANGRLENYRHHPSFNALKTVAKMLQGAQYIKPIATSAGLEIHYFVSNNQHTHA
                     VWATNGNAAFATDVYSAASLANANITDRDGNVLSHLELITESPVYLTWPTIEAIQVHA
                     KPRLAKDLRIHAHIQHQHYYTFAQHGWQGVLLANNAIEAEMLFSALHPHNLAAPAKDQ
                     ALRHARNAIWSIDSPRGDSTKITVKQPIKMHLHKAFFDRYKPSKAKRSWNGAMELLRR
                     GIATAMPVAYFEKLGDSTLKENFYLCEFVDHDFNIAAAFIAFSQGAQHFHGLTATELY
                     VAFADYCHLMHRRGIYFRDFSGGNILVKITDNQKLQFSLIDTARLHAYNHGTPFNYRI
                     ADLTRALNKLHWPGRTQLMRIYLAMSGRKLTWRTKLAFYLYDFKVDFKRKFGRKAVRK
                     LLKKFRSNSH"
     gene            complement(17837..19156)
                     /locus_tag="PKL42_00510"
     CDS             complement(17837..19156)
                     /locus_tag="PKL42_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HNU65576.1"
                     /translation="MLLSVGGLLSCASHETEPKVIVPPSPPVAKCQCDCPEKKCPPPP
                     TLPVTEPVTEPTPPKEIKTPEVKAAEAKIAEYGLLKPASWDEVDGFSNTKVNGDNLSL
                     AWPAWLQGCTTLVNKPVWKSVCSAATQLSNQTKNKPSSEKVKTYFKQNFSVYKTTNVD
                     GSDVGLITGYYEPLLKGSRTKSAQYSHPLHTTPSDLVTVELTDLFPELKFKRVRGRVV
                     GNKLLPYYTRAEIEASNAPLKAHEFIYIDDIIDVFYLQVQGSGLVQLDNGQQVHVGYA
                     DQNGHTYNSIGRLLVERGELTYANASMAGIKNWAKNNPTKLRELLNNNPSYVFFRELP
                     AGLPGPLGALGVPILAERVVAVDPKFVPLGAPVFVSTTEPNSAKPLKRLMMAQDTGGA
                     IKGGVRADYYWGSGPEAGAKAGAMKQEGKIWVFLPKDFVLPPPVAAP"
     gene            complement(19258..19641)
                     /gene="apaG"
                     /locus_tag="PKL42_00515"
     CDS             complement(19258..19641)
                     /gene="apaG"
                     /locus_tag="PKL42_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HNU65577.1"
                     /translation="MAAAKRYECSVTVEVEFLPDQSDITLNRYAFAYHIKITNTGNVT
                     AQLISRHWVIVEANGEKQEVKGLGVVGEQPLLLPNSHFEYTSGTVLNSPMGEMHGSYQ
                     MVAEDGSQFDVQIARFVLTMPRVLH"
     gene            complement(19641..20423)
                     /locus_tag="PKL42_00520"
     CDS             complement(19641..20423)
                     /locus_tag="PKL42_00520"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HNU65578.1"
                     /translation="MSDLLNIAGKHYQSRLLVGTGKYADFAQTRAAIDASGAEIITVA
                     IRRTNIGQNAGEPSLLDFLPPEEFTYLPNTAGCYSADDAVRTLRLARELLDGHKLVKL
                     EVLGDPNTLYPNMIETLAAAKTLVKDGFDVMVYCSDDPIIAKQLEDIGCCAVMPLASL
                     IGSGMGILNPWNLQIIIENAKVPVLVDAGVGTASDAAIAMELGCQGVLMNTAIAAARD
                     PVLMAAAMKKAVEAGREAYLAGRMPKKLYSASPSSPTTGLIT"
     gene            complement(20463..20666)
                     /gene="thiS"
                     /locus_tag="PKL42_00525"
     CDS             complement(20463..20666)
                     /gene="thiS"
                     /locus_tag="PKL42_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNU65579.1"
                     /translation="MQLIINGKSRDFESANLSVADLVSALKLESKRLAVEWNGEIVPR
                     SRFFETQLSDGDKLEIVGAVGGG"
     gene            20927..21094
                     /locus_tag="PKL42_00530"
     CDS             20927..21094
                     /locus_tag="PKL42_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65580.1"
                     /translation="MQVSAAVNTSAKATQPTQVKANTPTKDPVTHQASQPEAAKENSL
                     TRLLASCCDCV"
     gene            21213..21965
                     /locus_tag="PKL42_00535"
     CDS             21213..21965
                     /locus_tag="PKL42_00535"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNU65581.1"
                     /translation="MKVLSQTVDINTPTGVMRCYIHQPADSVNGTKQYPCILFYSEIF
                     QQTDPIERAAVMMASHGYAVLVPEVFHELNPIGTVLGYDEAGRNKGNTDKSTKDVQSY
                     DSDNVAMISYVKQQPWYNGNIGAMGFCIGGHLAFRAALQPDIKATACFYATDLNTQLI
                     PNKAGQHSMERLADITGELLMIWGRQDPYIPTPARAEIQAKLNAAQLNFTWHEFNAQH
                     AFMRDSDERYDAQTSLIGYQLALNLFSRKLSD"
     gene            complement(22117..23631)
                     /gene="gndA"
                     /locus_tag="PKL42_00540"
     CDS             complement(22117..23631)
                     /gene="gndA"
                     /locus_tag="PKL42_00540"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HNU65582.1"
                     /translation="MTTKNADIGLVGLAVMGQNLALNIADHGYTIAVYNRDSKKMTNF
                     IEECKNKEPSHERVVGHADLASFVLSIKRPRKIVLLVKAGSATDVTINALLPYLEQGD
                     IIIDGGNALWTDTIRREKELAVKGIDFIGSGVSGGETGARFGPSLMPSGTRKAWASLE
                     PIWRDIAAKVDAVTGEPLEGGKPGKPVEGGFSCAEYIGPDGAGHYVKMVHNGIEYIDM
                     QLICEAYWLMKNLLGMPADEIGKVFAEWNKGELSSFLIEITADILQQKDPMGKGFLVD
                     QILDTAGQKGTGQWTAANALELGAPANAIAAAVYARALSSLKEERVEASKILKGPEVK
                     KVEGDKKEIIEAIKNALYCSKICAYAQGFQLIDKAQVAYNWKLNFGEIAQIWRGGCII
                     RARFLQKITEAYSRNSRLKNLMLDPYFTQAMNDGQAGWRKVIAMAVTNGIPAQGFAAA
                     MAYYDGYRSADLPANLLQGQRDYFGAHTYERKDQPRGQFYHLDWPEAGRPQLEV"
     gene            complement(23818..24036)
                     /locus_tag="PKL42_00545"
     CDS             complement(23818..24036)
                     /locus_tag="PKL42_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65583.1"
                     /translation="MDNKYGLGSTKVAKEHHETHHKTPVSHATHCRFCGSVNLVLSST
                     MHDHRCADCGEWQIDVPQGYSTGRSSDF"
     gene            24211..24918
                     /gene="rpe"
                     /locus_tag="PKL42_00550"
     CDS             24211..24918
                     /gene="rpe"
                     /locus_tag="PKL42_00550"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009851956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNU65584.1"
                     /translation="MDKTFRIAPSILSANFAKLGQEIEDVIKSGTDIVHFDVMDNHYV
                     PNLTIGPLVCDAIRDISKNVGALIDVHLMVKPVDRIIPDFAKAGANIITFHPEASEHI
                     DRSLSLVREQGCKSGLVFNPATPLSYLDYVMDKVDMILLMSVNPGFGGQKFIPSTLDK
                     LKQARARIDAYYEKTGRQIWLEVDGGVNAQNIADIARAGADTFVAGSAVFGAGKDTDP
                     NRYNSVVKSLRDALATA"
     gene            24936..25604
                     /locus_tag="PKL42_00555"
     CDS             24936..25604
                     /locus_tag="PKL42_00555"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HNU65585.1"
                     /translation="MSQFKVKAVMFDLDGTLIHTAPEIASAANQMLADLGRPILPYQQ
                     IESYIGEGAQVLIKRCLCGKRDMEPEPALFEQGQTLFYHYYAVNAQESKPFDGVITGL
                     YALENKGLKMACVTNKPEKFTLPLLKNSGLDGFFDIVVSGDTLPNKKPDPIQLHHICA
                     KFNIMETDAMLVGDSATDIAAAHAAGCFIVTVPYGYNQGQPIDDRLVDATISDLTELV
                     TLLA"
     gene            25809..27293
                     /gene="trpE"
                     /locus_tag="PKL42_00560"
     CDS             25809..27293
                     /gene="trpE"
                     /locus_tag="PKL42_00560"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HNU65586.1"
                     /translation="MLNTINQDEFNALEKQGYNRIPLVLETFADLDTPLSLYLKLANQ
                     PFSYLLESVQGGERFGRYSIIGLPAKMRIVAHNRQIQVLQDNVVVESIDNTNPLDFIQ
                     QFQARFKTPPREGLPRFTGGLAGYFGYETIRYIEKRLANTAKPDAINVPDVLLMISEE
                     IAVVDNLSGKLYFIVYANPAEPQAYEKAHERLKGLVAQLRKSVAIPQAKSSTTTQATS
                     EFGEENFKSAVKKAQQYILDGDIMQVVLSQRMSQPFAESPLSLYRALRSLNPSPYMFY
                     YDMGDHHVVGASPEILVRLENGTVTSRPIAGTRPRGKTREQDLALAEELLADPKERAE
                     HVQLMDLGRNDVGRVAQTGSVKVTDNMMIERYSHVMHIVSNVEGKLKPNMDAIDVLKA
                     TFPAGTVSGAPKVRAMEIIDELEPSKRGIYAGAVGYLGFNGDMDVAIAIRTGVIKDKM
                     LYVQAGAGIVADSVPQSEWIETQNKAKAVLRAAEIVQAGLDSAS"
     gene            27318..27947
                     /locus_tag="PKL42_00565"
     CDS             27318..27947
                     /locus_tag="PKL42_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HNU65587.1"
                     /translation="MTTKHQHATAFLSNIDEDWAKLIATVGPCDFQAKEQREPFEALI
                     RAVAYQQLTVKVGDAILAKLISGFNSSAQNQFPTARQIIESSFDDLRQCGFSGRKIET
                     LKLIAEGTINGLVPTRMEASRMTDEALIERLIQLKGIGRWTVEMMLMFTLARMDILPA
                     DDFGIAQGYRRLKKLASAPSRKMLTEIAKVWSPHRTVASWYLWRVPREW"
     gene            28158..29402
                     /locus_tag="PKL42_00570"
     CDS             28158..29402
                     /locus_tag="PKL42_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNU65588.1"
                     /translation="MRTFTHPIFAVHQHAFLKLLTYRLFIVLAYQIMAVTVGWHIYEI
                     THDTLSLGLIGLVEVVPYFATALFAGHAVDHHCSRRFFGTLASFLLVASAVALAAISA
                     GWLGENIASTTLWIYMAFAFTGVARAFISPSYNALFALILPRDQYARAAGIGTSVFQI
                     GLVVGPALGGLLVGFASKTVAYMFAIFLCVVAIVAIWTIRVKEPQVEASPAVLKSIAE
                     GLRFVYHHQIILGALTLDMFAVLFGGAVALLPAFIQDIYKLGPEGLGILRAAPAIGAI
                     FTGLWLARHPINLHAGRWLLASVAGFGVSIIGFGLSSSIWLAGLMLLISGMFDGVSVV
                     MRQTIMQLATPDAMRGRVSAINGIFIGSSNELGAFESGLAAKLIGLVPSVIFGGLMTL
                     SVVGITAKLAPKLRNLELHQLH"
     gene            complement(29571..30197)
                     /locus_tag="PKL42_00575"
     CDS             complement(29571..30197)
                     /locus_tag="PKL42_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNU65589.1"
                     /translation="MNNNIQYFSLKTFSASASMLLGLFSAHAHADWVNLGNIGPTFTF
                     YENFNLDKSDYVSEENIASDSLTFTLNSAQTVTFFADNFGFENDYTEFRQLDILDANK
                     ISGVEDGAPYVAGIGSYELGLNGQESKFSYLLGPGDYQINYKVRGQEVDYDPDRNMGK
                     AKEGLGSYYLGLSIGGAYINPVPEPETYAMLLAGLLLVGFSVRRKRQV"
     gene            complement(30486..31532)
                     /locus_tag="PKL42_00580"
     CDS             complement(30486..31532)
                     /locus_tag="PKL42_00580"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNU65590.1"
                     /translation="MYINPLSALAALLLSANVAAESALNEVEVSANVSQIEANETAEP
                     VVISPVTLEKPAINTAEVKKGLNNPSAQLVAIALKYKDTEVANREYAKVAETYCRAAK
                     FGDADAQYALGWMYQNGRGVAADEKVAAQLYTMAAEQGHTRAKSTLATLADVKPESTL
                     LACLLPDPPPEPMVTTLPSVDGTNAENKGISEKTAALFYSKASILKIVNKLAPKYEID
                     ANLAMSFIAVESNFNVQATSPKNAQGLMQLIPATAQRFGVKDAYKAEENIKGGLAYLR
                     YLLAYFQGDVELVAAAYNAGEGAVEKYKGVPPYAETQLYVKKIARLYNNNSHPYKENW
                     VKESPFLKTKKTSG"
     gene            complement(31689..32750)
                     /gene="mutY"
                     /locus_tag="PKL42_00585"
     CDS             complement(31689..32750)
                     /gene="mutY"
                     /locus_tag="PKL42_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNU65591.1"
                     /translation="MPEFAARIITWQKLHGRHDLPWQTKLNQPSDPYAIWVSEIMLQQ
                     TQVAAVIGYYAKFMQRFPDIASLAQATQEEVLQYWSGLGYYSRARNLHLAATTVMDTH
                     DGIFPSDFSSIQSLPGIGRSTAAAIAAFAFNQRHTILDGNVKRVLARHFLIEGWPSAP
                     AIEKKLWALAESLLPAQEIQAYTQGLMDLGATLCTRSKPKCEACPLNTTCIAFAQQRV
                     NDLPTPKPRKIIPQKNTTMLILRCGDEVMLEKRPASGIWGGLWSFPEMNALHQIEGRQ
                     VENEVQKRFGCDVLSNQTLPKLTHTFTHFKLHIQPHLLDTLQNHASKDNAHLWLNIEE
                     AMGAAIPTAVRKILSSLQS"
     gene            complement(32743..33300)
                     /locus_tag="PKL42_00590"
     CDS             complement(32743..33300)
                     /locus_tag="PKL42_00590"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008057755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNU65592.1"
                     /translation="MQTLTLLPDNLPIPLDDGACSHLLGLELPDVILKSTSNDAINLS
                     AIDGWVVLFCYPRTGQPNQALPEGWDAIPGARGCTPQACAFRDDYQKITALGAKVFGL
                     SSQSSDYQKEASLRLHLPFSLLSDVDLLLTDALNLPTFVLAGMRLNKRVTLISFKGII
                     QQYFYPVFPPDKNSEYVLSWLREHA"
     gene            complement(33315..34898)
                     /locus_tag="PKL42_00595"
     CDS             complement(33315..34898)
                     /locus_tag="PKL42_00595"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HNU65593.1"
                     /translation="MAEVGYRHVAKYGKNNATNIVINIKADGQKLNLNPLSAKVDDSQ
                     IKGSFAISQFVQPAFHFDLDVDQVDVNRYVTADAGNAPKTDKPADLSALKTLLADGSL
                     RVGSLKYDKYQVGNLKLNLKADGQKLQINSLAAKVDDSQVNASIGISQFSNPVYNFNV
                     AIDKLNADRYIAKGEASNKKAGDTPLDLSALKKLNASGEAKIGWLKLANVKTENVDIG
                     LKAENGIATLSPFSANLYQGSMSGVLKVDARATPNIAFKQSMKNISVGPLLVDAINND
                     MLSGNGTLNVDVTTQGNTVSALKKALQGNAALNLANGALKGVDIAGSIRELKNKVNIL
                     GTKEANADKSKKTDFSELTATFAIKNGVAHNDDLAMKAPVLRLAKGDSRGDIDIGNEK
                     INYIAKPTIVKSLKGQGGADLDSLAGIAIPVKISGTFSDPKYGLDFAAVGTALAKSNL
                     LDKVGGEKGAAVKELLGNENKTDAISNIISGKKKTTEATPTEPAEDAAAAAPVEKPKS
                     LEEQAKEKAEKKLKKLLKF"
BASE COUNT         9577 a         7774 c         7814 g         9920 t
ORIGIN      
        1 gttgatcgaa tccggcgagc tttaaccaca tttcaaccgc aaattacgga tacccaaaat
       61 tactttaaaa aacacttgac agataaaact aaatatctaa ttatatagtt atgaaaatta
      121 ttcaaacgat tcctgatgcg tggcaaaaca tcgcgcagct gtttattgcc ctaggcgacg
      181 cgcaccggca acgcattttg ctgacttttg aaaaaggtga gcgcatgaat attatgcaaa
      241 ttgccgccgc ctctacttta agccgaactg ctgtcacaca tcatttaaaa atactgcgtg
      301 aaagcggcgc cctgcaaagt gaaaaaatag gtaaagaagt gtatttttgg gtggataaag
      361 gctttatgac taacgccatt agcaatgtat tgcattatat tgaaacagaa atttaatttt
      421 aatcgttaac taaaacataa aagttggggg acttgaaatg ctagccaagt tattgaggtt
      481 tatctgcaag attttgtttc gcgttgaggt gcgaggctta gaaaacgtac ctaaagaaga
      541 tgggttactt attgttgcca atcacgaatc atttttagat ggcctgctgc ttggtttatt
      601 tttacctaaa aaagcaactt ttgttgtaca tacttcggta ctcaaaaaat ggtcattcag
      661 gcaagcttta cgcttaactt cctacttagc ggttgacccc accagcccgt tggcgatgaa
      721 aaaggtcatt aaattgcttg aagcgggtga gaatgtggtg attttccctg aaggccgcat
      781 caccttaacg ggtaatctga tgaaagtgta tgacggtcca ggctttgtgg ccggcaaaac
      841 ggctgccgac attctgcccg tgcgcattga tggcgcggca gagtcttatt ttgggcgctt
      901 aacccgtcat cacccgcgca gattgctacc taaagtcacg ctcaccatca tgccaaccac
      961 cagtatcgcg atgcctaaag ccacccatca cggctttcct acaggtaaac agcgccgccg
     1021 tattgcgggt gaaggcatgc gtagcgtcat gcaaaaaatg ctgtttcaat cacaaaaaag
     1081 ccgcacctta tttgaggctt ttttggatgc taaagataaa tttggcgata actacaagct
     1141 gattgaagac atcaacgaaa cagaagaaac ctatcaaaat gtgctgaaaa aaagcctagc
     1201 actgggccgt attgttacca aagtcagtcg tgcaggtgaa accgtaggcg tcatgatgcc
     1261 taatatcact aacacagtgg cactatattt aggtatgagc gcgtttaacc gtgtgcccgc
     1321 catgcttaat tacacatcag gtacggcagg tatgcaaaat gcctgtattg ctgccaacat
     1381 taaaaccatt attacctcac acaagtttat tgaaacagcc aaattagaaa gcgtggtggc
     1441 aggcttaact aacctcaata ttttatattt agaagattta cgcgcgcagt ttggcgtggt
     1501 tgatcgtgct tggctcatcg gttatgccct acattatcca cgggcggcga tggaggtcag
     1561 caagccggat gaacctgccg ttgttctgtt tacatcaggc agtgaaggca agccaaaagg
     1621 cgtagtgcat tcgcataaaa gcattttgac caacatctcg caaattatgg cgatattaga
     1681 ttttaacgtc accgataagt ggatgctggt gttacccgtt tttcacgcct ttggttttac
     1741 tgcgtcgctt cttacgatat ataacggcat gaaaactgtg atttttccct cacctttaca
     1801 atataaggtg attccagaag taatttacga taaaggatgt accgtattta tcgccaccag
     1861 cacctttttg gcgaactatg ccaaatatgc acacccttat gacttttaca aacttcgcgt
     1921 ggtgattgcc ggtgcagaaa agctcaatga agaagtgcgc agaacctacc aagaaaaatt
     1981 cggcatacgc attttagaag gctacggcgc aacagagtgc gcgccggtgc tttcagcaaa
     2041 cacagcgatg gctaataaaa atggcagtgt cggtcaattt gtgcctggct tagagcatcg
     2101 cctagagacg gttacgggaa ttgaaagtgg tggcttgctc catgtgcacg gtgaaaacgt
     2161 gatgatgggt tattacttgt ttgataaccc aggggtgttg catccaccat cagaaggctg
     2221 gtacagcacg ggcgacatcg tagagttaga cgcacaaggc tatattcata ttaaaggccg
     2281 tgtaaaacgc tttgctaaag ttgcgggtga aatggtgtcg ctagaagtgg ttgaacatat
     2341 tgccaacacg gccgcaccag aacaccaaca cgcggccagc acgcaagagg atgcagggcg
     2401 cggtgaaaac atcattttat ttaccacaga cccagaatta aaacgtgaag acttacaaat
     2461 tgtggctaaa aatacaggtt cgcctgaaat tgccatcgcc cgcaaaattg tccgcgtaga
     2521 agaaattcca gtactgggta cgggtaaaac cgattacgtt acgcttaaac aaatggcgga
     2581 aaccgcgtga gaaaggcatt actcatcacg ctattgagtt tattgcttgt gccagtggcg
     2641 attggtgctt gggtaaagca gccttttaac catgaaatag gtgaattaaa atttgctggt
     2701 ttagcagagg ctgaccctga aagattaaaa gcagatgttg aaaaactctc agtaacgtat
     2761 gcaacgcgcc actttggcga tgtaagaacg ctggatttag ccgcaaatta tattcaggca
     2821 gaattcaaaa aaacaactga gcgcgtagaa atacaaagtt atcaagttga tagcgaaaag
     2881 tttcataaca tcattgccca ttttgggcca aaaattgact acaccgccac ttatgaaaat
     2941 ggcccaccca agcccatcat tattggtgcg cattatgatg tgcatgaaga caccatgggg
     3001 gcggatgata acgccagcgg cgtggcgggc ttgctagaat tagcgcgaat ttttgccaaa
     3061 aaaccacccg ccatacctgt gcaaattgtg gcttatactt tagaagagcc gcctaatttt
     3121 agaaccgata gcatgggcag tcgccaacat gtggctagcc taatgcaggc tggcatacag
     3181 cctgagttgg tgatggtatt agaaatgatt ggttactata acgaagctga aaatagccaa
     3241 gaatacccag tcagtttgct caaatggttt taccccacac gcggcgattt tattggcgtt
     3301 gttgggcgtt tttcagccgc acccaccaat aaaatggtga aaatggtcat gcaatcagca
     3361 catccagaat ttcctgtgga atccattagc gcacctgcaa tgatgacggg catagatttt
     3421 tctgaccatg catcctattg gttacacgat atgcccgcag tgatgattac tgacacctcg
     3481 ttttatcgga acccaaatta tcacaaagca actgacactg ccgatacgct agattacacg
     3541 cgtatggcaa aagtggttga aggggttcat acggtgacgg tttatatgag tgactcaaaa
     3601 aatattaaag ataaaagcga tgaataactt tatatcagca aaacaaatta ttgcattctg
     3661 tatatggtgt ttcacctcag cgcccttcat tgcgtatggc aataacacag aaaatgcgca
     3721 aaataatgca gaggtattaa cgcttaatag cccccaagct gtaacaggca aatatgaaac
     3781 tgatgcgcaa gcagtgctgg cttatattgc aagttcacaa cagctaagtg cgcaattacc
     3841 aagttttatg gttgaatcaa atcaagccat taaaaagcac aaaggcgcac taccctctgc
     3901 ctatgcgttg cgcttggcaa aggcgctttc tgaggcacat gatttgcgcg atggcttatt
     3961 taagcaagca cttactcacc gctcagcgct ttatagagtt gatgatgaca ttagtgataa
     4021 tgagcgggtg gaagaaatcg tcatagccat gtcagccgca gtgactttgt ttcaaaatag
     4081 caaaacgatg caccaagcct ttgataaaaa tccactatta aaaaataaat tgaatgaagg
     4141 ttacccagag tttggtattc cagaggggtt ttatgattca tccaccatgc gctcaaataa
     4201 cacagaatat cgtaaagctt ttaccgatgc agtacgatat ttcgcagata accgcgaggc
     4261 aatagaggcc gaaatcaatc gaagctcaac ttcaattcag tcgctatatc aacatattgc
     4321 taaaagccca atgttgacgg gttttaaagg ggccaatgta tttaaaagaa ttgccatttt
     4381 gccttttaaa gcagccaacg gcgtggtaga tgtatcaggc cgcagtttaa atcatgcaaa
     4441 atttaccagt tcaaaaattg tgggaaacac tatgggggca gtgcgctggc gcgccggtaa
     4501 attgaaagat gatgcgcaag tatttcaaac gatgctttcc caactgcaac caggcgatat
     4561 tttgctagaa aaaacgccat ttacgctcac tgataaaagt attcctgggc attttggtca
     4621 tgttgctgtg tatgttggaa gtacagcgca attaaaagaa ttagggcttt tagaacaagc
     4681 gattgtacaa aaaaatctag ccaaaattga acagggtcat agcgtcgttg aagctttaag
     4741 aagcggcgta caacttaata aattagaagc atttatgaat gtggatgacg tagcggtgtt
     4801 acgcccaaaa aacttgagca tagaagacca gcgccaagcg gtgacattgg cgcttggcaa
     4861 tttgggtaaa aaatatgatt ttaattttga tgtaaatact accgataaga ttgtttgctc
     4921 tgagctcgtt tatattgttt atccacaggt ggattttgaa actaaaaatg tcttaggtag
     4981 cttttcaatc accccagatg acattgccca gcgcgcgggc agtaatgaag cagacccgct
     5041 agaggtagta ttatttgcac atgatggaaa gctggtgtgc gagcaacaat cacccaataa
     5101 gcatgaaggg atagtcttat atgaacgttt ggtaaaatct gctgaaaaaa ccaatgctaa
     5161 caatgcaagt ggtgtacagc gtgcggcatt ttcaggattt ttataaaaat atttattaaa
     5221 tagccaaaat gactaagctg acaaactatc aatccacctc tgcattctca aatgcaatgg
     5281 tagggattaa agcatggcta gagactggtc atcgcaaccc taacttaaca ggcttgccag
     5341 agtcactcaa aacccatcca ctgatgcacc aagtttggga tggcattgat gcaactcagg
     5401 tgtgggattg ccacgcgcat attattggct ctggtgatag cggaacaagt ggcgcttggg
     5461 ctacgcccag gatggatact tggcgacacc ctatactaaa aattcaaaag tggtttcatg
     5521 tgaatggggc tgggctcgat agaaaaaatg aagacaccag ttttgtatcg cgtttggcgc
     5581 atttatctag cgagatgccc gagggctaca aaaccatgct ttttgccttt gaatgggcgc
     5641 acgatgaaac tggcaagcaa attaaagaag actctatctt tcatatccct aatgactacg
     5701 ccgcaagaat tgtggcgcaa catccgcagc attttgagtg ggtggcttct attcaccctt
     5761 atcgcgctga tgcgattgat gccttagagc aagcccacgc ccaaggtgcg gtggcgatga
     5821 aatggctggc ttcttctatg aatatcgacc ctgcatcatc aaaatgcacc aagttttacc
     5881 aaaaactaca acaactcaat atgcctatga ttacccatgt agggcgcgaa agtgccgtgc
     5941 ctggcggtga tttaaatgtt aataatccat taaaagcccg cgccatgtta gacgcagggg
     6001 tgcgcgtggt gatggcgcat tgcgccagtg atggcgttga caaagactat gatcatggca
     6061 ataagctagt cagcagtttt gaattgttta cccgcatgat ggataagcct gaatacgaaa
     6121 aattattgtt tagcgatatt tctgcaacga cattgtttaa tcatgcgtgg gtactcaaac
     6181 acgtattgca acgcaccgat tggcatcatc gcttgcataa cggcacagac tatccgttgc
     6241 caggggtttt tccgctgatt tctagcgagc aattttatcg tgaaggcatg ttggcaaaag
     6301 aacatgtgcc atttttgcaa gaagtgcgca aatataaccc catcatgttt gattttgcga
     6361 tgaagagatt actcagttat aaagaaaaac agtttccaac cagcgtattt gaaacgcgcg
     6421 gtttttttcg gcagttaatt taggagattt aaaatgaaac aactattgat ggcgacattg
     6481 ttggcgactg ctattgtctc acctttatca gcgttagcta atggctccgc agcctcactt
     6541 gatgcttcaa aaacactttc cgttggcgtt ggctctatcg ttgtagggtc tgcctcatta
     6601 gtcgcggcta gcgggcaaat agtggttgaa agcgtagaaa acaccgcaga tggtgtactt
     6661 atggtgttac acggtgtggg ggcaggtgta tcagaggcgg gtaagttctc gctaaaaatt
     6721 actggggatg tttcaggggc ggcctcagtg gctgtaggac attcagtgga agtggttgca
     6781 gagacagcag gatcagccat tattgcctca ggcaaagtta tcgcatttat cccaaatgcg
     6841 ctgggcaaat cattaatgca tcattcagaa ctataaaatg tataacttta aaattcagac
     6901 ctattaaatt caatcctata aaatgatgaa aaaacaatta ctaagcctgt tgctgtgttt
     6961 aatgctgcat ccagtatttg ccgaggctgg gacggcttgc actgaaaagc cacaatctgc
     7021 tgaaaccgtg caaaaggctt ttcaactagc gattaaaacc cgcgaggctt tagatgcgag
     7081 cggtgccgag gttgccttgg ttgggcgtgt agggcaagat ctatcaaagt ataatctgcg
     7141 ctattcgcat atggcttttg tgtggcgcga ccatccgcag gggcgttggt tggcagtgca
     7201 tgaactcaat caatgcggaa ccgccaattc agcattattt aatgaagggc tcgcgaactt
     7261 tttctttgat gatatgtttg cttgggatgc ccttatcgtc acgccatcac caaatatgca
     7321 ggcaaaaatc gtggctttgt taaaaaatac cgcgtattta aaacatgttc atgagccaca
     7381 ttacaacatg gtggcgcacc ctttttcaac aaaatatcaa aattcaaatc aatgggtgtt
     7441 agaaatatta tccagcgcta tgagtgagga gttattcact agccgaagtg ctacacaaac
     7501 ttggttgaag caggcaaact tcaagcccag tgtgttgcgt attcctacac ttaagcgttt
     7561 aggtggccgc atgtttcaag caaatgttgc atttgatgac catccaaatg cagatcgctt
     7621 tgcgggcaat attgcagttg ttacggtgga atctgtaaaa gaatttgtca agcagcaaga
     7681 tagtgcagta caagaattaa cagtgagctt gcagtaaatt aagagggaaa tcttatggaa
     7741 ttggctttgc taggtttaat tggggtggca ttaggtgtca tcatattagt gggttcaatt
     7801 gctggctttt ttggcatgtt ttcatcaagc gataaagcaa aaaagcttta tcagctagag
     7861 caagaaaccg aaaaattacg caaagaaatg gcacgtatta gcgcacaaat tgacgcgctc
     7921 aaagccaacc cgccgcaaac aacacctgtg caaaccgctg cgcttaaggt ggcagataca
     7981 ccaacggttt cagttagcga gccaattgta gcgtcagtat tagaaaaacc aatggcggaa
     8041 agcgctattt cagccgttgt gcaaccaaca gttaagcttg agcctacaac tagaactatt
     8101 gaaaaaacca tcgaaaaacc aattgaaaaa ccagcttacg ttaagcctgc gtatacgccg
     8161 ccgccgccaa atttcattga gcgaggtttt gcagcggcta aagagtggtt atttggtggc
     8221 aataccatgg tgcgtgcagg tattgtgatt ttatttatcg gcattagctt tttactcaag
     8281 cttgcggtag ataatggcat gattccaatt gagtatcgtc tgcttgccgt ggcaatgggt
     8341 ggcatcgcat tgttgatagt tggttggcgt ttgcgtgata cccgctcgca atatgcttgg
     8401 gcgttgcagg gcggtggtgt gggcatttta tacctcacca tttttgcggc catgaaaatg
     8461 tatggactga ttcctgcagg cgcggcattt ggattgttag cagccattgc gtttttatcc
     8521 gcgtttattt cggtgaagca atctgctttg ccactggcta ttttaggctt tgcaggtggt
     8581 tttttagcgc caattttaac ttcaactggc agtggaaatc acattggctt atttagttat
     8641 tatttagtgc ttaatttagg aattgcttac gtggctttat ataaaagctg gcgttcacta
     8701 aatgtgctgg gatttgcttt cacctttatt attggttcaa tttggggcgc aaaaagttat
     8761 acgcctgacc tttttgccac cactgagcct ttcttaattg cacatttctt actatttacc
     8821 gtcattgcaa tcttattcgc gcatcgacaa tccaccaaag cgagcgatta tgtagatgcc
     8881 actttggtgt ttggtacgcc attggttgcg tttagtttgc agtacgcatt gcttaaaggt
     8941 acaccgtttg gcttagctta tagtgcttta gccttgggga ttttctattt aggcttggca
     9001 tggtgggtgt taaatcgcca acgcgaaacg cttaaatttt tgggcgagtg ctttttagcc
     9061 ttaggcattg gatttgtcac gctcacctta ccactagcgc ttgatggccg ctggacatcg
     9121 gccgcttggg cagttgaagg cgtgggctta ttgtgggtag ggttaaaaca aaatcgcaaa
     9181 tttcctgctt ttacgggact ttccttgcaa ttacttgccg ctttggcttt tgcaaagggc
     9241 tggggtttaa caggttacgg tgggtttggt catcaaaact tattcttagg tgttggcttt
     9301 attgcgctat caggttgggc atgtggtgcg ctacttaatg catatcgccc aaataaattt
     9361 gattggatgc gtacgccttt agctgtttgg ggttggttat ggtgggtagg tgcgggctta
     9421 acggccatca gtgagtttat gccacgcgat aattttatgc atgcaggttt aatttttgtt
     9481 gcactgacca gtgcgttatt accttttgtg gctaataaat tgaaatggtc gcgcttagcg
     9541 acgctttcca ctttattgtt gcctgcaatg ggtatcgctt tgtgctggga agcaattctt
     9601 acactcttta gttatagtag ctattctatt ctaaaaaata acggatttat cgcatggccg
     9661 ctcgcaatcg gggcttatgc ttggcttatt tcacgtaatg ctatccctga gaaagcgtgg
     9721 ttgcgcgcgc cattactatg ggtgatggcg gctgttggtg tgctggagtg gcatcaccag
     9781 ttgggtcaat atgtgagtga atcgcacgtg tgggcgcaaa tcggctgggg ggtgataccg
     9841 atgctgatta ttggcgcagt tacctactgg caatttgcaa aaaaccaatt aaatgccaca
     9901 cgccttgata gcgcacgcac ttgggcgtgg gtgggcagtt tgccgctcat ggcgctcact
     9961 gtcgtttggt atttcaatat gtcactcaat gccagcggaa acgccgcacc actgccttat
    10021 tttccgattg tgaatccgtt agatataacg ctggcaggtg cattgttatt gttgctgatt
    10081 tggcagcgcg cagtgagcaa gcatttaggc aagttgcaac atattaaccc cgtgttagcc
    10141 agtttaatgg ggtttgcgct actcaatggc atgctgttac gcacactaca tcattggatt
    10201 ggcacgccgc atttatgggt atctatattt aataattcta ccgtacaaat ggcatttacc
    10261 tttttgtggg gcatcacggc gtttgggttg atgttacttg ctcacaaaaa agagcgccgt
    10321 tcattatgga ttgtaggcgc tagcctgatg gggttggtgg ttcttaaact attcttactt
    10381 gatctctcac aaagcggctc agttgaccga atcatctcat ttattggggc ggggatgatg
    10441 ttattggtga tgggctattt tgcacccttg ccgccagcca ttaaagccaa tgaattaaag
    10501 gaatcagcat gaagtcattt aaaaaaatta tcgccactgc ggccttatgt tttagtggtc
    10561 ttgtgtctgc tgaaagtttt aatctcaata ccacaagtag cgaaccgctt tatcagagtc
    10621 tacttacaaa agaggtttat gctgccagcc accaagacca cttacaagac ttaacgatta
    10681 tcaacgctag tggcgagcaa gtaccttatg ccctgttgca ctattatgat ttgcatccac
    10741 aaacggccac ttcaatcaaa cgtcagccgc tcagcgtgtt tcccgttcag gaaagcagcc
    10801 ttaataatcc taatgcactg acgatacaac ttgaaaatag cgctgaatct tctaacgtga
    10861 gtctcaatat aaataatgca acggcagatg ccaaaaccgt ctttttggtt gatgcaggta
    10921 aaaaacaccc acccttacaa acattaagtt ttgattggca agggagcgaa ggcgtattgc
    10981 tgtcattaga agtgcttgca agtgatgact taaaaaattg gtctagtgtg gggcatggtg
    11041 tattactcaa aaaaactgct gaaggtaacg ctattttaca aaacagtatt acattagatt
    11101 accctacaga agcgcgttat ttgcaagtac gctctacaaa gaatgaagcg cttcagctca
    11161 gtcgtgtaga tgctgcatac aacaatgtgc gcgcgcttac gccagcaatc atgtggcaaa
    11221 cggttcagtt tgtacaacgt gagcaagaca ataaaaatgg agtcgtcaac ctcgactttg
    11281 aagcgctagg ccgctacccg gccagcttcg tgcaagtgaa acttccacaa gacaacacaa
    11341 ttacgagcgc cagcattttt gtgcgtaata aacaggatgc gccatggcaa tacatcgcca
    11401 caacttcgct ttatcagatg aatcaggcca ataaaaccta taccaatcca gatattttaa
    11461 tcaatagcac gacggcacgt ttttggcgat tagcgtttaa ccaagccaac ggcggtattg
    11521 gccaacaaaa tccacaatta aacttaggct ggctgccgca gacggtggtg tggaacgcac
    11581 gcggacaagc cccttttaaa ttagaagttg gtgaaaagcc tagcgtcgtc aacacagttt
    11641 cagtcgctaa tttaattcca gatttcaaaa tagagaaggt gcatcagctt ccagtggcaa
    11701 atatcagcgc accagttgcc agtgcggtag cgcctcagcc caaaaacacc tggacagcag
    11761 cccctgatta taaaagctgg atgttatggg gcggtttgtt gctgggtgtt atgttgcttg
    11821 cgggcatggc ggtttcattg cttaaaagtg acaacaaacc ataaaacacc cctgatcacc
    11881 atggaataac ttcagatgag caatcagttt cagctattgc gtgaacaacg ctttcgcccg
    11941 ttctttttta cacagttttt gggtgcgttt aacgataatg tttttaaaac cgcgctaatc
    12001 accttggttg catttcatgc ggctagcctg acgacgatag atggcggcac gctcgccacg
    12061 gtgttgccag gcttgtttat tttgccgttt tttttattct ctgcaacggc tggccaactc
    12121 gcggataagt ttgaaaaatc gcagattatt cgttatgtga aggtgtttga aattgccatc
    12181 atgttatttg ccagcctcgg gttttatttt catcatattg gcttgctggc tgccgcgctt
    12241 tttttgatgg gtacacattc aaccattttt ggcccagtga aatattctta cctgccgcag
    12301 catctaaaac aaaacgagct tgtcggcggc aacggcatgt tagaaatggg gacgtttgtg
    12361 gcaatattgc ttgggcaagt gttgggtgct tgggtcgcca tgcaacctca gcatgaaatc
    12421 atcaccagtc tggttatctt actcattgcg gtcatgggtt atgccaccag tcgcggtgtg
    12481 ccaaaatctc cacccgcggc tccgcatcta aaaattaact ggaacccagt cactgaaacg
    12541 taccgcaata ttcaatttgt gtggcaacac cagcaaattt ggctcgcgat tgtggcgatt
    12601 tcatggtttt ggttttttgg cgcaacgctg cttgcacagt ttcctaattt tgcaaaagtg
    12661 gtgctttatg gcgatgaaag cgtttttatt ctgttgcttt ctgttttttc tattggcatc
    12721 ggcatcggct cgctgatgtg cgaaaagctc tcaaaaggca aggtggagtt gggtttagtg
    12781 ctgtttggcg ccatcggttt aaccgtgttt ggggctgatt tatatagctc aagcacttgg
    12841 atacacgcgc attggaacaa caaaacgtta tttgattttg caagttttat cagcaggcat
    12901 catgacatgc acggccaagt gatttttgcg cactggcgtt tgcttgccga cattgcgctg
    12961 ataggctttt ttggcggact ctttattgtg ccgctgtatg cactaataca aacgcgcgct
    13021 gaaaaaagcc accaatcgcg ggtgattgct gccaataaca tcatcaatgc tttttttatg
    13081 gtggcttctg cagggttttc aattttaata tttcacaatg aattgacgat tccagagctt
    13141 tttttagtca cggcactact gaacgtgctg gtgatgattt atctgtgctt taggcaaccc
    13201 gagtatttca aagcgtttat tcgatggatt tcattgggta aataagttgt ttattggtca
    13261 tgtaaaagcg aggctaaaat aaccgcattt gaatgcttat ttactatcaa cccttttcac
    13321 ctcctctgcg agccttataa ataaaggctt gcagagcatc aaaaatctct cgggaaaatt
    13381 tactacattt ttgattgttt ttttattgct tcaaccccac attaaaactg gcgattttgc
    13441 aatcagctag gccaataaat cgctttcact ttataatggc aacaatcact ttttggtgcg
    13501 aacctgatgc aacttattca tcgaattaaa caatccattt ggcaattggc aaacaagtgc
    13561 ggcgcgtttg cacccttatt tgctatgctg ttgctgggtc tcattttatt gtcactttcg
    13621 cgcgtgggct tggtgctttg gaagctcgat cgtgtgctgg ccaccgataa attggtcggg
    13681 atttttttgc aagggatacg tgttgatatt atccagctaa gcctgatggc gcttattcca
    13741 ttattgctgg cacccatttt agcgacaaag cagttattta aagtttggca aaaatttacc
    13801 tatatttggg taacgatctc cattttactg ctggtctttt tagaaatcgc tacgcccggc
    13861 tttattatgg aatatgacgt tcggcccaat cgtatttttg ttgaatacct taaatatccg
    13921 aatgaagtca tgagcatgct ttggacgggc tttaaagtgc atatttttgc aacactgggc
    13981 tttagcttaa tcaccctttg gctcatgcgc cgatttatga agccttggct agcagcaaag
    14041 ccaacgtggt ccaacaaaaa gctttggctg atttggccgc tgatattttt gctttcagcg
    14101 ttcgcgattc gttcttcact ggggcaccgc ccggccaacc cggctttatt tgccatcacg
    14161 caagacagca tggtcaatag cctcattcta aattctggct attctgttat ttatgcagtg
    14221 tatagcctgc agcatgaggc aaaatctagc aaaatttacg gcaaaatggc caaagaagat
    14281 atttttaaaa tcaccggtgc caaagacacg gacttgccaa ccctcacaac gctcaacccc
    14341 agcgccaaac gtgataaacc gctcaatctg gtgattattc tacaagaaag tttgggcgcc
    14401 acttttgtgg aatcgttagg tggcgtgcca gtgaccccaa acttagaaaa actaaaagaa
    14461 caaggcatct ggtttgagca actgtacgcc acaggcacgc gctcggtgcg cggaattgaa
    14521 gcggttgtta caggctttca gccaacgcct gccgacagca ccgtgaaatt atcgttaagc
    14581 caaaaaaact tttttagcct tgccggcttg ctgagtaagc acggttacag cacagagttt
    14641 atttacggtg gcgagtcaca ctttgataat atgcgcggct tttttatggg caacggtttt
    14701 caatctatta tcgaccaaaa agattataaa aacccggtga tgctgagtag ttggggcgta
    14761 tcggatgaag atttactgaa taaaacccat gagcaattgc tggcgcacca tgccagcggt
    14821 aaaccgtttt ttacactggc tttttcatct tcaaaccatg cgccgtttga gtttcctgat
    14881 gggcgaattg aactgtatga gcagccaaag aataccgaca ataacgcagt gaaatatgct
    14941 gattacacca tcggtgagtt tatcaaaaag gctaaggcca gcccttactg gaaagacacg
    15001 gtgtttttga ttgtggcaga ccatgatatt cgcgtgcgtg gcgacacact ggtacccatc
    15061 gagcgctttc atatccccgg gctaattttg ggcgctgata tcgcgccaca gaaaattacc
    15121 gccatggcaa gccaaatcga tttgcccgtc acgatgttat ctctcatggg tttacagacc
    15181 cagcacccga tgacaggaca tgacttatca caaatcccag cggactatct tggccgcgcc
    15241 atgatgcaat ataactataa tttcgcctgg atggagcaaa cgcctaccgg caaccaagtc
    15301 gttgtgctac gtgaaaataa agcccctgcg catggggtgt atgacactaa agtaaaacgc
    15361 ctgcatgaag taagtccacc acaaaatgcc atcacccttg aacagcgcgc gctggcgaat
    15421 acactcctgc ctgctttatt atattcggag caaaagtacc gagtgcctga atgacgaaaa
    15481 tagcagaaaa gaaacagcct tctatgctga ccaaacaagt catttttgat ggcttgcgct
    15541 acattttaac cggtgggtta cgcatcaaaa aaggccattt gcctgttcag gcacatcaaa
    15601 ttccacatga ttttatcggc atttgcgttg ccagcaatca agatgccgcg gtggatgact
    15661 acgttgtggc caacattaaa cttttaggta ttcaacgggt tcgcttagat tttacctata
    15721 ctgatgtaga taactatcaa gtccggtttt taaatcgcct gattgcagaa ggctttgacg
    15781 tcaccctgca ccttttgcag ccgtttgagg acgccaaaaa catgcaatcg gttgaggcac
    15841 aattgaaatg gcgcaaattt ttggtgacgg tgttagatac ttttggccaa aaaatcaaag
    15901 cagtcgaatt tggtaacacc ctcaaccgca agcgctgggc aggctatgat tggtctggct
    15961 ttatgcaagc gtggcgcatc gtgcatcaag aaatcaccag ccgaaatatc acactgattg
    16021 gccccaatgt tcaagacttt gagccacttt acaacgtgag tttgctcaaa acgtttaaac
    16081 agcaaaaact gctacccgat attcacagca acaacttatt tgtagaacgc gttaccgaac
    16141 cagagcgttt tgaccaccgt attctcaagt acagatgggc aactttgctc aaatacaatt
    16201 tagtcaaaaa agcgcgcatt ttacaaaaaa ttggccaaga ttttggcgtg ccacagacgg
    16261 tttcatcggt cgctttttgg gcaatttatc gcattcagcg ctttttgccc gacggcgaac
    16321 aaaaacaagc agattatgct gcacgctact ttttactgct ggccgcatcg ggcgcgctcg
    16381 caagcgctaa ttggggtgcg cttatttgcc agcgtgaagg tgtgattgat gatggtctta
    16441 ccgagcaaga gtaccctgac ctagaacgcg tggcgcatta tcagcaagcc aacggacgat
    16501 tagaaaatta tcgccaccac cccagtttta atgccttaaa gaccgtcgca aaaatgctgc
    16561 aaggggcgca gtacatcaaa ccgattgcaa caagcgctgg cttggaaatt cactattttg
    16621 tatcaaataa tcagcacacg cacgcagtct gggccaccaa tggcaatgct gcttttgcga
    16681 cagatgtcta ttcggcagca agcctggcca atgccaatat cactgatcgg gatggcaatg
    16741 ttttatcgca cttagaactt attaccgagt cacccgttta cctcacttgg ccaacgattg
    16801 aggccataca agtgcatgca aaaccacgct tggccaaaga tttaagaatt cacgcccaca
    16861 ttcagcatca gcactattac acctttgcac aacacggctg gcaaggggta ttgcttgcca
    16921 acaatgcgat agaagccgag atgttattta gcgcactaca cccgcacaat ttagccgcac
    16981 cagcaaaaga ccaagcgttg cgccatgcta gaaacgcgat ttggtcaatt gacagcccgc
    17041 ggggagattc aaccaaaatt acggttaaac agcccattaa aatgcatctg cataaagctt
    17101 tttttgaccg ctataaaccc agcaaagcca agcgcagttg gaatggggca atggaattac
    17161 tgcgccgagg cattgcaaca gccatgcctg tggcctattt tgaaaaactt ggtgacagca
    17221 cactcaaaga aaatttttat ctatgcgagt ttgttgacca tgattttaat attgcagcgg
    17281 cttttatcgc ctttagccag ggcgctcaac attttcatgg tttaaccgca accgaactct
    17341 acgtagcttt tgcagattat tgccacttaa tgcatcgtcg cggcatttat tttagagatt
    17401 tttctggcgg caatattttg gtaaaaatta ccgataatca aaaactgcaa ttttcactca
    17461 tcgataccgc gcgcctccat gcctacaacc atggcacgcc gttcaactat agaattgctg
    17521 acctcacccg tgcgctcaat aaattgcatt ggcctggcag aacgcaattg atgcgcattt
    17581 atttggcaat gagcggccgc aagttgactt ggcgcaccaa actggcattt tacctgtatg
    17641 actttaaagt ggactttaaa cgaaaatttg gccgcaaagc ggttagaaaa ttacttaaaa
    17701 aattcagatc aaattcgcat taaaaccctc gagaaaaatt taagcctctg gaagccttgt
    17761 aaataaaggc tcgcagagca tcgaaaaagg ttgtgggtta tttgaagtgt aaaatcagcg
    17821 ttaaaaacct gattgcttaa ggtgctgcaa cgggtggcgg tagtacaaaa tctttcggta
    17881 aaaataccca aatcttgcct tcttgcttca tcgcccccgc ttttgcgcca gcctctggcc
    17941 ctgaacccca ataataatct gcacgcacgc cacccttaat ggcaccgcca gtatcttgtg
    18001 ccatcatcag ccttttaagc ggctttgcac tatttggttc agtggttgaa acaaacacgg
    18061 gggcgccaag tggcacaaat ttggggtcaa ccgctacaac ccgctccgct aaaataggca
    18121 cgcccaacgc gcctaacggg ccgggcaatc ctgctggcag ttccctaaaa aatacgtagc
    18181 ttggattatt attgagtagt tctcttaatt tagtagggtt attttttgcc caatttttaa
    18241 tgccggccat agaggcattg gcgtaagtta attcaccccg ctcaaccagc agacggccaa
    18301 tggagttgta ggtgtgcccg ttttggtctg cataacccac atgcacttgc tggccgttat
    18361 caagctgcac caatcctgag ccctgcacct gtagataaaa aacatcaata atatcgtcaa
    18421 tataaataaa ctcatgtgcc ttgaggggcg cattgctcgc ctcaatttct gcacgcgtgt
    18481 aataaggaag cagcttattt ccgaccactc gcccacgcac ccgcttaaat ttaagttctg
    18541 gaaataagtc agtgagttca accgtcacca aatcactggg cgttgtgtgt agaggatgcg
    18601 aatattgcgc agattttgtg cggctgcctt taagcaaagg ttcatagtaa ccggtaatta
    18661 aaccaacatc tgacccatcc acattggtgg ttttatacac agaaaaattt tgcttaaagt
    18721 aagtctttac tttctctgag ctcggcttgt tttttgtttg attgctcaat tgcgtggcag
    18781 cactgcaaac gcttttccac actggtttat tgacaagcgt tgtacaacct tgcagccaag
    18841 caggccaagc caaactcaaa ttgtcaccat tgactttagt gtttgaaaag ccatccactt
    18901 catcccatga agcaggtttt aataaaccat actctgcgat ttttgcttca gccgctttaa
    18961 cttcgggcgt ttttatttct ttaggcgggg ttggttcggt aacaggctca gtaaccggta
    19021 atgttggtgg cggtgggcac tttttttcgg ggcagtcaca ttggcatttc gcaacgggtg
    19081 ggcttggcgg cacaattact ttaggttctg tttcatggct ggcacacgag agtaaaccgc
    19141 ccacagaaag caacatggcc aacttaccaa accacaaaaa aggattttta ttattcaatg
    19201 gaacccaact taattaaaaa ttaaaacacc atatacaacg caatgctgta cgcattgtta
    19261 atgcaacacc ctgggcatgg ttaagacaaa gcgcgcaatc tgcacgtcaa actgtgagcc
    19321 atcttctgca accatttgat agctgccgtg catttcgccc attgggctgt taagcactgt
    19381 accgctggta tattcaaaat ggctatttgg tagcagcaaa ggttgttcgc ccacaacccc
    19441 taaccctttc acctcttgtt tttcaccatt ggcttctaca atgacccaat ggcggctaat
    19501 taactgcgcg gtaacgttgc cggtattggt gattttaata tggtaggcaa acgcataacg
    19561 attgagcgtg atgtcagatt ggtctggcag aaattccact tctaccgtta cgctgcattc
    19621 atatcgtttt gccgcagcca tcacgtaatc aacccggttg tcggcgagct tgggctggca
    19681 gaatataatt tcttaggcat tcggcctgcc aaataagcct cgcgcccagc ttctactgcc
    19741 tttttcattg cggcggccat gagtacgggg tcacgcgctg cggcaatcgc ggtgttcatc
    19801 aaaacgccct gacaaccgag ttccatagcg attgcagcat cagatgcagt gcccacacct
    19861 gcatccacca acaccggaac ttttgcgttt tcaataataa tttgtaaatt ccaagggttt
    19921 aaaataccca tgcctgagcc gatcaaagag gccaaaggca tcaccgcgca acagccaata
    19981 tcttcaagtt gcttggcaat aatcgggtcg tccgaacagt acaccatcac atcaaagccg
    20041 tcttttacca gcgttttagc ggcagccagc gtttcaatca tgttggggta cagcgtattt
    20101 gggtcgccaa gtacttccaa cttcactagt ttgtggccat ctaatagctc acgcgctaag
    20161 cgcagtgtac gcaccgcgtc gtcggcagag taacagcccg ctgtattggg cagataagta
    20221 aattcttcag gtggcagaaa gtccaacaag gaaggctcac ccgcattttg gccaatattg
    20281 gtgcggcgaa tcgctacggt aataatttca gcgccactcg catcaatggc cgcgcgcgtt
    20341 tgggcaaagt ctgcgtattt tcctgtgccc actaacaacc ttgattgata atgtttaccc
    20401 gcaatattca ataaatctga caattttatt ccttatcgtt taattcgttt gcctgcgtgc
    20461 cactaaccgc cacccaccgc gcccacaatt tccagcttat cgccatcgct caactgcgtt
    20521 tcaaaaaacc gactgcgtgg cacaatttca ccattccatt ctacggccag tcgcttgctt
    20581 tcaagcttaa gcgcgctcac caaatcagcc accgataaat tggcactttc gaaatcacgt
    20641 gactttccgt taatgatgag ttgcatagtg aaaaaagcaa taaatgatga aaaagggatt
    20701 cagaaacaaa tatggttgcc gttattaatg acgaagctag ataagcgttc attttacgaa
    20761 aatagtagtg tgtttagcaa accttatctg gaatattcgc tgtcatccaa aatgtgatga
    20821 ccagcgttat gttgcgtgtg attacattca cattatttta atagattact ttaactttca
    20881 tgaaacacaa tgacatgaag gtaaagctcc caaaaggaaa aacaaaatgc aagtttcagc
    20941 agcagttaac accagcgcaa aagcaactca accaacgcag gtcaaagcaa atacgcctac
    21001 taaagaccca gtaacgcatc aagccagcca acctgaagcc gctaaagaaa attcacttac
    21061 acggcttctc gcctcttgtt gcgattgcgt ctagattttc tagcgccata aaacgcacgg
    21121 caccgcccgt gcaaatttat gctttgggcg tttacgcttg acaaagtaca atatcaaaat
    21181 tatcaactcc ttgttaattt tgagagatta ctatgaaagt cctcagccaa acggtagaca
    21241 tcaacacccc aaccggggtg atgcgttgct atattcatca acctgctgat agcgtcaacg
    21301 gcactaaaca atatccctgt atattatttt attccgaaat atttcaacag accgacccca
    21361 tcgaacgcgc cgccgtaatg atggcaagcc acggctatgc cgtattggtg ccagaggttt
    21421 ttcatgaact aaacccaatt ggcaccgtgc ttggctatga tgaagccggc cgtaacaaag
    21481 gcaacacgga taaatccacc aaagacgtac aaagctacga cagcgacaac gtagccatga
    21541 tttcctatgt gaagcaacaa ccgtggtata acggcaacat cggcgccatg ggcttctgta
    21601 ttggcggcca cctggcgttc cgcgccgcct tgcaaccaga cattaaagct accgcctgtt
    21661 tttacgccac cgacttaaat acgcaactca tccccaataa agctggccaa cacagtatgg
    21721 agcgcctcgc tgacattacc ggggaattgc tgatgatttg gggcaggcaa gacccttata
    21781 ttccaacccc cgcgcgggct gaaattcaag ccaaattaaa tgcagcacaa ctaaatttca
    21841 cctggcatga atttaatgcg caacacgcct ttatgcgcga tagtgatgag cgttatgatg
    21901 cgcaaacttc gctgattggc taccaacttg cgcttaattt atttagccga aaattaagcg
    21961 attaagccta aatttcaaac aacccttttg tgctgctctg taagccttta tttataaggc
    22021 tcacagaggc ataaaaatct ctcgggcgaa tttagcaaat atgtagatgt aaaaaagggc
    22081 agaatttctg ccctttttag tttgctagat atgcaattaa acttctaact gcggacgacc
    22141 tgcttctggc caatctaagt ggtagaactg accgcgtggc tggtctttac gctcataggt
    22201 atgggcgcca aagtagtcgc gctgaccttg caacaagttt gctggcaagt ctgcacttct
    22261 ataaccatca taatatgcca ttgcagcagc aaagccttgc gcaggaatac cattggtgac
    22321 tgccatggca atcactttac gccagccagc ttggccatcg ttcattgctt gggtaaagta
    22381 tgggtctagc atcaagtttt tcaaacgtga gttacggctg taagcctcag tgattttttg
    22441 taagaatcgt gcgcgaataa tacaaccgcc gcgccagatt tgtgcaattt caccaaaatt
    22501 gagtttccag ttgtatgcca cttgtgcttt atcaatgagt tggaagcctt gtgcataagc
    22561 gcaaattttt gagcaataca aagcgttttt aatggcttca ataatctctt ttttgtcgcc
    22621 ttccactttc ttcacttctg ggccttttaa gattttgctt gcttcaacgc gctcttcttt
    22681 taggcttgaa agtgcacgcg cataaacggc tgctgcaatt gcgttagccg gtgcgccaag
    22741 ttctagcgca ttggccgcag tccattggcc tgtacctttt tggcccgcgg tatctaaaat
    22801 ttggtcaact aagaagcctt tacccattgg gtctttttgt tgcagaatgt ctgcggtaat
    22861 ttcaatcaag aaacttgata attcgccttt gttccactcg gcaaatactt taccaatttc
    22921 atctgctggc atgccaagta gatttttcat caaccagtag gcttcgcaaa tcaattgcat
    22981 gtcgatgtat tcaatgccgt tatgtaccat ttttacatag tgaccagcac catctgggcc
    23041 aatatattca gcacaagaga aaccgccttc aactggcttg ccaggtttgc caccttctaa
    23101 cggctcgcct gttaccgcat ctaccttagc ggcgatgtcg cgccagatag gctctaaact
    23161 tgcccaagct ttacgtgtgc ctgatggcat gagtgatggg ccaaagcgtg cgcctgtttc
    23221 accacctgaa acgccagagc cgataaaatc gatgcctttg actgcaagtt ctttttcacg
    23281 acggatggtg tctgtccaca atgcgttgcc gccgtcgata ataatatcac cttgctctaa
    23341 ataggggagc aaagcgttaa ttgttacgtc tgtcgcgcta cccgctttaa ccagcaacac
    23401 aattttgcgc gggcgtttaa tacttaacac aaatgaagct aaatcagcat gacccacaac
    23461 acgctcgtgt gagggttctt tgtttttgca ttcctcgatg aaattggtca tcttttttga
    23521 gtcacggtta taaaccgcaa tggtgtagcc gtggtcggca atattcagag ccaaattctg
    23581 gcccattaca gcaaggccca ctaggccaat atcagcattt ttcgtagtca tttatcttct
    23641 cttttggagt tgtttaatgg aaagggtgtt tttgtacgca agattataga acttttaacg
    23701 gcagtgtttg ttaaaacttg tgccaattca gtggattttt tgattttttt tgattttttg
    23761 ttaaaaacga atcgtcacga tgaaacgttt cgatgttcat caatgcattt ggaggggcta
    23821 aaaatcacta cttctacctg tggaataacc ctgtggaaca tcgatttgcc attccccgca
    23881 atcagcacag cgatggtcgt gcatggtaga gctgagcacc aaattgactg agccgcaaaa
    23941 ccgacagtgc gttgcatggc taacgggtgt tttgtgatgc gtttcatggt gctctttggc
    24001 cactttggtg ctacctaagc catatttgtt atccatgatg acctcctata gataaatacc
    24061 aaagttaaat gtcaattgaa taactaaatt acgctttttt aattctgctt gcaagtttta
    24121 aatgcgcttt aagcattcgt cacccgaaaa ggataaatca agttaaaata atggtttgat
    24181 gctattttta actaactgga accacccatc atggataaaa cttttagaat tgcccctagt
    24241 attctttctg ctaattttgc caaactcggt caagagattg aagatgtgat taaatctggc
    24301 acagacatcg tgcattttga cgtgatggac aaccattatg tgccaaacct gacgattggc
    24361 ccattggtgt gtgatgcgat tcgtgatatt tcaaaaaacg ttggtgcctt gattgatgtg
    24421 cacctaatgg taaaacctgt agaccgtatt attcctgact ttgcaaaagc aggcgccaac
    24481 attattacct ttcacccaga agcttcagag cacattgacc gtagcctttc attggtgcgc
    24541 gagcaaggct gtaaatcagg tttggtgttt aacccggcca cgccgttaag ctatttagat
    24601 tacgtcatgg ataaagttga catgattttg ttaatgtctg tgaacccggg ttttggcggt
    24661 caaaaattta ttccaagtac attagataaa ctcaaacaag cgcgcgcacg tattgatgca
    24721 tactatgaaa aaactggccg tcaaatttgg cttgaagtag acggtggcgt gaatgcacaa
    24781 aacattgctg acattgctcg ggcaggtgca gatacattcg tagcaggctc tgcggtcttt
    24841 ggggcaggta aagatacgga ccctaatcgt tacaacagcg tagtgaaatc actgcgtgat
    24901 gcattagcaa ccgcttaact ctcgagcttg ggcgcgtgag tcagtttaaa gtgaaagccg
    24961 tcatgtttga tcttgacggc acactcatac acacagcgcc agagattgca tcggcagcga
    25021 atcaaatgct ggccgactta ggtaggccga tattgcctta ccagcagata gaaagttata
    25081 ttggagaagg ggcgcaggtg ttaatcaagc gctgcttgtg cggcaagcgt gatatggagc
    25141 ctgaaccggc tttgtttgag caaggccaaa cgctgtttta tcattactac gcagtcaacg
    25201 cgcaagagag caagcctttt gatggcgtga taaccggcct gtatgcactg gaaaataaag
    25261 gcctgaaaat ggcctgcgtg acgaataaac ctgaaaaatt caccttaccg ctccttaaaa
    25321 atagcggctt ggatggtttt tttgacatcg tggtatcggg cgacacacta cccaataaaa
    25381 aacctgaccc gatacagctt catcatattt gtgcgaaatt taacatcatg gaaacagatg
    25441 ccatgctcgt gggcgattct gcaactgaca ttgcagcagc acatgcggct ggctgcttta
    25501 tcgtgaccgt gccctatggg tataatcagg gtcagccaat tgacgatagg ctggtagacg
    25561 caaccatttc agatttaact gaattggtca cattgttagc ctagcattca gcaaagctaa
    25621 atcattaatt tttaaaattg tattgatatg aacgtaataa aacatcattt ttaccgcaac
    25681 ttacaacagg ctgccctatg ctggcgccgc tcttggcaac gttaatgttt aggtgacaca
    25741 tataaaaacg agtcactagt ttgatgacta gttcaattta aaaaatgatg agaaagtctt
    25801 aaaagtttat gttaaataca attaaccaag atgagttcaa tgcgctagaa aaacaaggct
    25861 ataaccgcat cccgctcgtg ctagaaacct ttgccgattt agacacgcca ctttcacttt
    25921 acttaaagct cgctaatcag ccattttcat acctgctgga atcggttcag ggcggcgagc
    25981 gttttggccg ttattccatt attggcttgc cagcaaaaat gcgaattgtc gcgcacaatc
    26041 gtcaaattca ggtgctacaa gataatgttg tggttgaaag cattgataac accaatccgc
    26101 ttgattttat tcaacaattt caggcgcgtt ttaaaacacc accacgtgaa ggcttgccac
    26161 gctttaccgg cggccttgca gggtattttg gttatgaaac catacgttac attgaaaagc
    26221 gtttggccaa cacagctaag ccagatgcga taaacgtacc tgacgttttg ttgatgattt
    26281 cagaagaaat tgcggtggtt gataatctat caggtaagct ttattttatt gtctatgcta
    26341 acccagcaga gccacaagct tacgaaaaag cccacgaacg cttaaaaggc ctagtggcac
    26401 agttgcgaaa atctgtggca attccgcagg caaaatctag caccaccacc caagcaacgt
    26461 cggaatttgg tgaggaaaac tttaagagcg ccgttaaaaa agcgcaacaa tatattttag
    26521 atggtgacat catgcaggtg gtgctttcac agcgcatgtc tcaacctttt gccgagtcac
    26581 cgttatcact ttatcgtgca ttgcgtagtt taaatccctc accttatatg ttttattacg
    26641 acatgggcga ccaccatgtg gtgggcgcgt cacctgaaat tttggtgaga ctcgaaaacg
    26701 gcacggtcac ttcacgccca attgctggca cacgcccacg cggcaaaacg cgtgaacagg
    26761 atttagcgct cgctgaagaa ctgctggccg accctaaaga gcgtgccgaa cacgtgcaac
    26821 tcatggactt ggggcgaaat gacgttgggc gggttgcaca aacagggtca gtaaaagtaa
    26881 cggacaacat gatgattgag cgctactctc atgtgatgca tattgtgagc aatgttgaag
    26941 gtaaacttaa acctaatatg gacgccattg atgtacttaa agcaacattt cctgcaggca
    27001 cggtttctgg tgcgccaaaa gtccgagcaa tggaaattat tgacgaatta gaacccagca
    27061 agcggggtat ttacgcaggt gcagtaggat atttaggctt taatggtgat atggatgtgg
    27121 caattgcgat tcgtacaggc gtgattaaag ataagatgct gtatgtgcag gcgggagcag
    27181 gcattgtggc cgattcagtt ccgcaaagtg aatggataga aacgcaaaat aaagctaaag
    27241 cagtattacg agctgcagaa atagtacaag caggtttgga cagcgcttca taaatatcta
    27301 aaccaccctt gctcgtgatg acaacaaaac accagcacgc aaccgcgttt ttaagtaaca
    27361 tcgacgaaga ctgggccaag ctcatcgcca cagttggccc gtgcgatttt caagcaaaag
    27421 aacagcgtga accttttgag gctttaattc gcgcggttgc ttatcaacag ctaacagtca
    27481 aagttggcga tgctattctg gctaaactca tcagcggctt caattcaagc gcacaaaatc
    27541 aatttcccac agcacggcaa atcatcgaaa gctctttcga tgacctgcgt cagtgtggtt
    27601 tttctggcag aaaaattgaa acgctcaaat tgattgcgga aggcacaatc aatggcttag
    27661 tgcccacaag gatggaagcc agccgcatga cggatgaagc gctgatagaa cgcctaattc
    27721 aactaaaggg cattggccgg tggaccgttg aaatgatgct catgtttacg ctggcacgta
    27781 tggatatttt gcccgcagat gattttggca ttgcacaagg ctatcgacgg cttaaaaagc
    27841 tagcatctgc accttcccgt aaaatgttga ctgaaattgc taaagtctgg agcccacacc
    27901 gcacggtggc aagctggtat ttatggcgcg taccacgcga atggtgaaga tactctcgaa
    27961 gcgttaaggg ttagtgcgcc taatctatat cggcagcgcc tcactaggtc aaagacgact
    28021 catcggcaag cataaatctt gcttactgcc ttattcacag cactaaccaa gacattttac
    28081 gcgtaatttt gctatgttct ggagtttatt cacgcctata ccgcgctttg aagagggttt
    28141 aattttataa tacttgcatg cgcactttta cacacccgat atttgccgtt catcaacacg
    28201 cttttctaaa attacttaca taccgactct ttattgtact tgcctaccaa atcatggcgg
    28261 taaccgtcgg gtggcatatt tatgaaatta cgcacgacac actttctctc ggcttaattg
    28321 ggctggttga ggtggtgcct tattttgcca cggcactttt tgcagggcat gctgttgatc
    28381 atcattgctc acgtcgattc tttggtacac ttgcctcgtt tttgcttgtg gcgagtgctg
    28441 ttgcgttggc cgctatttcc gctggctggt tgggtgaaaa tatagccagc accaccctct
    28501 ggatttacat ggcgtttgca tttacgggcg tagcccgggc atttatctcg cccagttaca
    28561 acgcgttatt tgcgctcatt ttgccaaggg atcagtatgc gcgcgccgct ggcatcggca
    28621 cctcggtttt tcaaattggc ttggtggtag gccctgcgct aggtggactg ttagtcggct
    28681 ttgccagcaa gactgttgcc tatatgtttg ccattttcct ttgcgttgtt gccatcgtgg
    28741 ctatttggac catacgtgtt aaagagcctc aagttgaagc gagccccgcg gtattaaaaa
    28801 gcatcgccga agggcttcga tttgtctatc accaccaaat tattctgggc gcactcacgc
    28861 ttgatatgtt tgcagtgtta tttggcggcg cagttgcgct gttaccggca tttattcaag
    28921 atatctataa acttggccct gaaggattag gcattttgcg tgccgcaccg gcgattggcg
    28981 cgatatttac tggattatgg ctggcgagac accccatcaa tttacatgca ggacgctggc
    29041 ttttagcctc agtcgcgggc tttggtgtaa gcattatcgg ctttggtctt tctagcagca
    29101 tttggcttgc gggtcttatg ctacttattt ctggtatgtt tgatggtgtg tcagtggtaa
    29161 tgcgacaaac catcatgcag cttgccacgc ccgatgccat gcgtgggcga gtgtcagcca
    29221 tcaacggtat ctttattggc tcatccaatg aacttggggc gtttgaatct ggccttgctg
    29281 caaaattgat tgggcttgtt ccctcagtca tttttggcgg actcatgaca ttaagcgtgg
    29341 tggggataac tgcaaaatta gcccccaaat tacgcaactt agaattgcat caacttcact
    29401 aaagtgtgct gcacttcaaa tttgagagtc cccagttaaa aatgtgcaaa attgtgccta
    29461 tatggcaact acccgttttc gatgctctga gagcctttat ttacaaggct ctcagagcat
    29521 ttattttttc tcgggagaaa ttaagctaaa ttaaaatata gctgatgcaa ttagacttgg
    29581 cgcttacggc gtactgaaaa accaacgagc aacaaaccag ccaacagcat tgcatacgtt
    29641 tctggttctg gaacaggatt aatataagcg cctccaatac ttaaccccaa atagtaactg
    29701 cctagcccct ctttcgcttt gcccatattt cggtctgggt cataatctac ttcctgacca
    29761 cgtactttgt aattgatttg atagtcaccc ggtcctagca aatatgaaaa tttagactct
    29821 tgtccattta gacctaattc ataagaacca ataccggcaa cataaggcgc tccatcttct
    29881 acaccactaa ttttattcgc atctaatata tcaagctgac ggaattcagt atagtcattt
    29941 tcaaatccaa agttatctgc aaaaaaagtt acggtttgtg cagaattcaa cgtaaatgta
    30001 agtgaatctg aggcaatgtt ctcttcgctg acatagtcac tcttatcgag attaaagttt
    30061 tcataaaagg taaaagtagg gccgatattg cccaaattta cccaatcagc atgtgcatgc
    30121 gcactaaata agccaagcaa cattgatgca gatgctgaga aagttttaag tgaaaagtat
    30181 tggatgttgt tattcataat agaagtcctt ttagatttcc cctgaaattt aaaggctttt
    30241 gaaagcaatt cataaaccga actgctggta ttaaaatacc ttattgatat tgattttaaa
    30301 ctgtctgcta gtaactaact caccgaggtt ggtgggttaa aaacgactag ctggacttag
    30361 tttacaatat ctctttcaca tttgcctata agaaaattgt tcgaaaatgt atctggaatg
    30421 ttgctgaaaa tgtttcgcta taaaaattaa tcaaaaaaac ttaggtataa aacaccaaaa
    30481 gaacattacc ccgatgtttt cttagttttc aaaaatggag attctttcac ccaattttct
    30541 ttataagggt gtgagttgtt gttataaagt cgtgcaattt ttttcacata caactgtgtt
    30601 tcagcgtatg gcggcacacc tttatacttt tcaaccgcgc cctcacctgc gttgtaggca
    30661 gccgctacta actccacatc accctgaaaa taggcgagca aataacgtaa ataagctaaa
    30721 ccacctttaa tattttcttc cgctttatag gcatctttaa cgccaaaacg ctgtgcggtg
    30781 gccggtatta actgcattaa accttgcgca tttttaggtg aagtcgcttg tacgttaaag
    30841 tttgattcaa ccgctataaa agacatcgca aggttggcat caatctcata ttttggcgct
    30901 aatttattca caattttaag aatgcttgct tttgaataga acaaagccgc tgttttttct
    30961 gaaatgcctt tattttcagc atttgtgcca tccacgcttg gtaatgtcgt caccatcggt
    31021 tctggtggtg ggtctggcaa cagacaggcg agcaaagtgg attcaggttt tacgtcagcg
    31081 agcgttgcca aggtgctttt agcgcgtgta tgaccctgct ctgctgccat ggtgtagagt
    31141 tgcgcagcaa ctttttcatc agccgcaacg ccgcgaccat tttgatacat ccaaccgagt
    31201 gcatattgtg catctgcatc gccaaattta gcagctctgc aataagtttc tgccactttt
    31261 gcatattcac ggttggctac ttcagtatct ttatacttga gggcgatggc cactaactga
    31321 gcacttggat tattcagccc ctttttcact tcagccgtat tgatagcggg cttttctaaa
    31381 gtaacgggtg aaatcactac cggttcagca gtctcatttg cttcaatttg agacacattc
    31441 gctgaaactt caacttcatt taaagcagat tcagccgcta cattagcact caacaacaat
    31501 gccgccaatg ctgataaagg attaatatac aataagatag cgttaatttt catctaaata
    31561 aatttttctt gaattggttg gcaatatgtt ttgcataagg attaagcaaa agctatgcct
    31621 attaggtgtg gattgtagtg tttgagcagg tttgcgcaat aacccgtttg ggctaatttt
    31681 tcaagcagtt acgattgaag cgagctcagt attttacgaa ctgctgtagg aattgcagca
    31741 cccatcgcct cttcaatatt aagccaaagg tgtgcgttat ctttagatgc gtgattttga
    31801 agcgtatcta aaagatgtgg ctgaatgtgc aacttaaagt gggtaaaggt atgcgtcagt
    31861 tttggcagtg tttggttgga taacacatca cagccgaaac gtttttgaac ttcattttcg
    31921 acttggcggc cttcaatttg atgcaatgca ttcatctctg gaaagctcca taagccgccc
    31981 caaatgcctg aagcgggcct tttttctagc atcacttcat cgccacaacg taatatcagc
    32041 attgttgtat ttttttgagg aataatttta cgcggtttag gcgttggtaa atcattaacc
    32101 cgctgttgcg caaaggctat gcaggttgtg tttagcggac atgcctcgca ctttggctta
    32161 ctgcgtgtgc aaagcgttgc gcctaaatcc atcaaaccct gcgtataagc ttgaatctct
    32221 tgcgctggca ataaactttc tgccagtgcc cataattttt tttcaatcgc tggtgcgctg
    32281 ggccaaccct caatcaaaaa atggcgggct agcacgcgct taacgttgcc gtctaaaatg
    32341 gtatgtcttt gattaaatgc aaaagcagca atggccgctg cagttgagcg gccaatccct
    32401 ggcagacttt gtatggatga aaagtcgctc gggaaaatac catcatgtgt gtccatcacg
    32461 gtggtggccg ccaggtgtaa atttctagcg cgcgagtaat aacccaaacc gctccaatac
    32521 tgcagcactt cttcctgtgt ggcctgcgct agcgatgcaa tatcaggaaa acgctgcata
    32581 aatttggcat agtaaccaat cactgctgcc acttgcgttt gctgtaacat gatttcagac
    32641 acccaaatag cgtaagggtc gcttggctga ttgagtttag tttgccacgg taaatcgtgg
    32701 cggccatgca gtttttgcca tgtaattatg cgagctgcga attcaggcat gctctctaag
    32761 ccaactcaac acatattcag aatttttgtc tggaggaaaa acaggataga aatattgctg
    32821 gataatgcct ttgaatgaaa tgagggtaac acgtttatta agtcgcatac cagccaaaac
    32881 aaatgtaggc aggtttagag cgtctgttaa taataaatcg acatcactca ataatgagaa
    32941 tggcaaatgt aaacgaagtg atgcctcttt ttgataatct gatgattgcg agcttaagcc
    33001 aaaaactttg gcacctaatg ctgttatttt ttggtaatca tctctaaaag cacaagcttg
    33061 aggggtgcat ccacgcgctc ctggaatagc atcccagcct tctggcaaag cttgatttgg
    33121 ctgtccagtt ctaggataac aaaaaagtac aacccaacca tcaatagcac ttaaattgat
    33181 agcatcattc gaagttgatt ttagtattac atcaggtagc tctaagccta acaagtgact
    33241 gcaagcacca tcatctaaag gaataggtaa gttgtctggt aacaaagtca gcgtctgcat
    33301 actattctac tgatttaaaa cttcaatagt tttttgagtt ttttctcggc tttttctttc
    33361 gcttgctctt caagtgattt tggcttctct acaggggctg cggctgccgc atcctctgca
    33421 ggttctgttg gtgtggcttc cgttgttttt ttcttgccgc tgataatatt gcttattgca
    33481 tcggttttat tttcattacc taaaagctct ttgactgccg cgcctttttc gccacctact
    33541 ttatccaaca agtttgattt tgccaatgcc gtgcctaccg ccgcaaaatc gaggccatat
    33601 tttgggtcag agaatgtacc ggatattttt actggtatcg caatgcctgc caagctatct
    33661 aaatcggcgc caccttggcc tttgagtgat ttcacaatcg tgggcttggc aatatagtta
    33721 attttttcat tgccaatatc aatgtcaccg cgactatcgc ccttagccaa tcgtagaaca
    33781 ggtgccttca tggcgaggtc atcattgtgt gcaacaccat ttttaatagc aaaagttgcc
    33841 gttaattcac taaagtcagt tttcttagat ttgtctgcat tggcttcttt tgtacctaga
    33901 atattgactt tgtttttgag ttcgcgaata gagcctgcaa tatctacgcc ctttaaggcg
    33961 ccattggcta aattgagcgc agcattaccc tgcaaagcct tttttagcgc gcttaccgtg
    34021 ttgccttgcg tggtcacatc tacattcaag gtgccgttgc ctgaaagcat gtcgttgtta
    34081 atcgcatcta ccaacagcgg ccctaccgat atgtttttca tactttgttt aaaggcgatg
    34141 tttggcgttg cacgggcatc tacttttaaa acaccactca tactgccttg atacaaattg
    34201 gctgaaaatg gtgataacgt ggcaatgcca ttttcagctt ttaagccgat atccacattt
    34261 tctgttttaa catttgccaa tttaagccag ccgattttcg cctcgcctga agcgttcaac
    34321 ttttttagtg cggataagtc cagcggtgta tcgcctgctt ttttgttgct ggcctctcct
    34381 tttgcaatat atcggtctgc attgagttta tcaatggcca cattaaaatt gtaaacaggg
    34441 ttactaaatt ggctaatgcc gatgcttgcg ttaacttggc tgtcatccac cttggccgcc
    34501 aatgaattga tttgcaactt ttggccatcg gccttgagat ttaacttgag attaccgact
    34561 tgatatttgt cgtatttcaa actgccaaca cgtaaactac cgtcagccaa cagtgtcttc
    34621 aaggcgctca agtctgccgg tttgtcggtt ttgggggcgt tgccggcatc agccgttaca
    34681 taacgattca catcaacctg gtcaacatct aaatcaaaat gaaacgctgg ttgcacaaac
    34741 tggctaatgg caaaactgcc cttaatttgg ctgtcatcca ccttagcact tagcggattt
    34801 aaattaagct tttgcccatc tgccttgata ttaatcacaa tattagtggc attgtttttg
    34861 ccatattttg ccacatgtct gtaaccgact tcagccacgg gaacactgaa gattcattga
    34921 aaggaaattg tttgccttcg tcagccatgg ttacctcatt ttgatcagtc attttggaaa
    34981 aatgtttgaa aaataacaat tatgcgtgac taatttttag tatagaagaa aatatttgtc
    35041 aatgtatttc cgccagtttg ggtgcgaatc taaacctgtt ccagc
//
LOCUS       DAOQSI010000004        34761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_664_length_34761_cov_5.346540, whole genome shotgun sequence.
ACCESSION   DAOQSI010000004 DAOQSI010000000
VERSION     DAOQSI010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 34761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34761
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_664_length_34761_cov_5.346540"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PKL42_00600"
     CDS             <1..328
                     /locus_tag="PKL42_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNU65594.1"
                     /translation="VTIMTISVTERTNEIGLLVALGARQRTILGLFLSESVILAVLGG
                     AMGLLFGIGLAQLLHLLMPNFPVHTPWRFAVVALLVSGVIGLIAGVLPAVSASRLDPI
                     DALRAE"
     gene            344..1429
                     /gene="corA"
                     /locus_tag="PKL42_00605"
     CDS             344..1429
                     /gene="corA"
                     /locus_tag="PKL42_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNU65595.1"
                     /translation="MRKHKKIRNKKIGLPPGSLVYVGDADIKHLDTLKITQITYDADG
                     IVEAELAISELANFKQDPNKKIWLNVHGVHDVDVIKKLGDLFNLHPLVIEDILNTEQR
                     PKIDEFDDYVFLETRHFYYHPENLSVSSEQISLVLGKYFLLTFQERSTGAFAPIRERL
                     RASHSNIREQGVDYLAYALLDSVVDRYFSVLEAVSDASETLEEALLANPTNAELHNIH
                     QLKHASIELRRAVWPLREVINSLTRNEKGFFQPSTLPYLRDVYDHTVNFIESLESIRD
                     SLSGMMDIYMASVSQRVNLEVRALTVVAMLFMPATLIAGIFGMNFHWMPWINDRNGFW
                     WALAIMSGIALMMILIFWRRQWLSSKT"
     gene            complement(1426..1965)
                     /gene="msrA"
                     /locus_tag="PKL42_00610"
     CDS             complement(1426..1965)
                     /gene="msrA"
                     /locus_tag="PKL42_00610"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNU65596.1"
                     /translation="MSNQQIAIFAGGCFWCTEPVFSQLHGVSSVVSGYIGGHTMNPTY
                     KSICNGDTGHAEAIQITFDASQISFETLLEVFFVSHDPTTLNRQGNDIGTQYRSAVFC
                     QNQAQEDAVKKMIATFSEADSFGAPIVTQINGADIFYPAEDYHQYYFDKNPANPYCIA
                     VAAPKAAKIRAKYAQLIKT"
     gene            2097..>3078
                     /locus_tag="PKL42_00615"
     CDS             2097..>3078
                     /locus_tag="PKL42_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HNU65597.1"
                     /translation="MALNALYVDFNSYFASVEQQLVPELRGKPVGVVAVMAETTCCIA
                     ASYEAKAFGIKTGTGVRDARKLCPDMVFVQARPPIYVALHHELIEIVESCTHVEKVLS
                     IDEMVCKLTGSQQLPENALKLAAKIKRAISKKFDTIRCSIGIAPNTFLAKTASNMQKP
                     DGCVLIEQHELPQRLFHLKLRDLNGIGKQMEARLNRYKITTVEQLYAANRQQLQTAWG
                     SIEGERMYDKLRGLEPYYVKNARSSLGHSHVMPPEQRNEAGVRAVLHRLLQKACMRMR
                     SYDLLASKISIKVKFRDKPSWNMESAISATDNTLRLIEALESFLQHYPKTQ"
     assembly_gap    3079..3178
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3179..3433
                     /locus_tag="PKL42_00620"
     CDS             <3179..3433
                     /locus_tag="PKL42_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HNU65598.1"
                     /translation="KTQHEPYAVGVNFSGLVTAEEVARDLFQVEPLENEKKLNQAIDA
                     LNLKFGKNTLYFGGAHDALKDAPMRIAFNHIPDLKVEGDT"
     gene            complement(3699..4745)
                     /locus_tag="PKL42_00625"
     CDS             complement(3699..4745)
                     /locus_tag="PKL42_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNU65599.1"
                     /translation="MNRIKQLFLVIGLTFTSFAFAGEFGDHCTTGLSKGSLVKTKCEI
                     NEIYQGKTYCFGNQAAKDAFLLDPDNTIAKASAFYSKNAEPMRDKISQADALKQINSK
                     TCDLSNKDAGYLIFNGLDLSHCNMQNISFFGAELMGANLSGANLQGAYLNLARLENAN
                     FSKANLTNATIFQAIFDKTNFAEANLTNARLIGTLGNVDLHKANLTNGQLGLDLGNQP
                     MGQMKFDSVGGNFTDSNFENANLGVAAFRFGNLRGANLRNSNLYRADLIKADLTGADL
                     TGANLTDAEVDDAIFKDVKGLETTKGFNTAKGKCIDCAVIATKLETKIVARTDLMTAE
                     NPAIKVCNIRGARF"
     gene            5094..5777
                     /locus_tag="PKL42_00630"
     CDS             5094..5777
                     /locus_tag="PKL42_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HNU65600.1"
                     /translation="MTGFKLNLVNNMRTKMLVPALLTAVFAAPAMANDQYRDTARVLS
                     VTPQTERVNVPRQECRTEYQQQSYSHGANNNVTGAIIGGVAGGLLGSTIGRGNGRVAA
                     AAVGAGVGALAGNSIANNNNYRHSTRTVPVESCYQVDNWQTVNTGYLVSYEYNGRNYT
                     TVTNEHPGRYIDVNVAVAPRSRAVTQVTYAQPHVSYGDSRWEDDDHRHRGHRGKHRDW
                     DRRGDGRYY"
     gene            5933..6460
                     /locus_tag="PKL42_00635"
     CDS             5933..6460
                     /locus_tag="PKL42_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65601.1"
                     /translation="MPNFMQQSVEFFKRLFLKKTRPNAAVAWQQLGHDTAGAYWLYAA
                     PVHLVLQRDTFSLAAPVPLPLEQPEIDTLTTLLNTHFGSDGIQFYWHENLWFLRLAQH
                     PQIQTSLPESALNQDIHAFMPTGEGAMRWAQVVNEMQMLLFEHPVNNAREARHLPAMN
                     SLWCYGGGQLPQSPK"
     gene            6470..8176
                     /gene="recJ"
                     /locus_tag="PKL42_00640"
     CDS             6470..8176
                     /gene="recJ"
                     /locus_tag="PKL42_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNU65602.1"
                     /translation="MQIKPRTFDENIVSTLIAEGVNPLLAKLFAARGVANKHALETSL
                     GQIIPPTLLTNNTAMAKLLADAIAQNKHLLVIGDYDADGATATAVAVKGLRAFGARVD
                     FLVPNRFEYGYGLTPEIVALAAQQKPDVIITVDNGIASVEGVAAANALGMQVLITDHH
                     LPGQITPQAACIINPNQHGCDFPSKHLAGVGVMFYGLLALRAELRARGAFTLTPEPNL
                     TELLDLVALGTVADLVKLDDNNRILVEQGLRRIRAGACSLGISALLRVSGRQPQQVNA
                     QDLGFYVGPRLNAAGRLDDMRLGIQCLLAETEVEAATIAQQLHTLNLQRRSIEADMQE
                     GAQLSLDDIAVESQFSISIYQDDWHQGVIGILASRIKERHHRPVIAFANAGDGLLKGS
                     GRSIAGLHLRDALDLLAKHQPDLILKFGGHAMAAGLTIKLVDFERFNHSFEAVVKSLI
                     TEADLASVLEVDGSLAADVMTFETAQALENQVWGQGFAPPLFFDCFEVVSQRVLSEKH
                     LKLTLKKENALIEAIYFNCAEAFAGAIFAAYQLQTNSYNGAQKVQLNLKYATCEKPLF
                     AS"
     gene            8380..9303
                     /locus_tag="PKL42_00645"
     CDS             8380..9303
                     /locus_tag="PKL42_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNU65603.1"
                     /translation="MQTRICTKQPLLKVTLLSAVLLAVLGAGCAKEVKAPQQAAVERQ
                     KITGLKTPESVVQAKDGRIFVSEIGEFGKAGDGVITLIDAQGKTSVYASGLDDPKGLA
                     VIGSDLYVADNHKVLKITSDGKSSVFASAEAFSGALHGSPYFLNDLEADPQGNLYVSD
                     SGDLFNTGLGGAIFKINPQGQVSLLLSNKQDVRIKAPNGLLADDTGEVLLVVDFSSGI
                     LYSLNQKTQVLTDLAEGFGGGDGLVHHSDGTMYVSDWKSGKVFSVSTKGDVTEIKSGY
                     QAAADIAITKDEKYLMVPDMKAGELDFILLK"
     gene            9351..10142
                     /locus_tag="PKL42_00650"
     CDS             9351..10142
                     /locus_tag="PKL42_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNU65604.1"
                     /translation="MNELRQNALHWLACSQVADKVAGVTALNLAWQAGDVCLDASIQL
                     TANQAIPGRPAKPVLVSPLAVEKRSMRTLDGRAALIHAIAHIEFNAINLALDAVWRFT
                     DMPKTYYANWLKVAAEEAYHFNLLHSHLQQMGYGYGDFSGHNSLWEMAERTNDDVLAR
                     MALVPRTMEARGLDASVPLRNKFAQIGDTAVADILDIILRDEIGHVAIGNHWFNWLCA
                     ARQLSAIATFEQLCQQYKAPKLRGPFNLEARRLAGFSEAELALLG"
     gene            10178..10789
                     /locus_tag="PKL42_00655"
     CDS             10178..10789
                     /locus_tag="PKL42_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HNU65605.1"
                     /translation="MTTNHRLFFPILLLILVAVFVWSAMQPADYLTWFLEVAPVLIAL
                     PILCFSYQKFPLTPLLYVLILVHAVILMIGGHYTYAEVPAFNWLRDTFELSRNYYDRV
                     GHFAQGFIPAMVAREILLRKNVVQKGAWLFFIVCCIALSISAVYELIEWWVAVASGKA
                     AEAFLGTQGDVWDTQWDMFIALIGAVIAQLVLANSHDRQLQTL"
     gene            10943..12106
                     /gene="carA"
                     /locus_tag="PKL42_00660"
     CDS             10943..12106
                     /gene="carA"
                     /locus_tag="PKL42_00660"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNU65606.1"
                     /translation="MSQNLPAILVLADGTVFRGTSIGASGQTVGEVVFNTSMTGYQEI
                     LTDPSYTKQIVTLTYPHIGNYGVNLEDVESGQVYASGLIIRDLPLIHSNFRNTQSLSD
                     YLVDNNIVAIADIDTRKLTRILREKGAQTGAVIAGEADETKALALANDLIAGFPGLNG
                     LDLAKVVTCEKPYEFTEGEWALGQGFTPAPAKQFHVVAYDYGVKRNILRMLVSRGCKV
                     TVLPAQTSAAEALAYKPDGVFLSNGPGDPAPCDYAIAAIKTIVDSGVPTFGICLGHQL
                     LALASGAKTIKMKFGHHGANHPVQDVETKRVYITSQNHGFAADATTLPSNIKTTHVSL
                     FDGSLQGIARTDKPAFSFQGHPEASPGPTEMSYLFDRFISMMQASKTAERKAS"
     gene            12106..15312
                     /gene="carB"
                     /locus_tag="PKL42_00665"
     CDS             12106..15312
                     /gene="carB"
                     /locus_tag="PKL42_00665"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNU65607.1"
                     /translation="MAKRTDIKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPVTWQVVEKIIAKERPDALLPTMGGQTALNCALDL
                     DKHGVLAKYKVELIGASKEAIDKAEDRQKFKEAMTKIGLGSARSAIAHSMEEALQVQA
                     SIGYPAIIRPSFTMGGSGGGIAYNREEFITICERGLDASPTKELLIEESLLGWKEYEM
                     EVVRDSADNCIIICSIENLDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAINPDDGRMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNEITGGATPASFEPSIDYVVTKIPRFAFEKFPQADSRLTTQMKSVGEVMAIGRTFQ
                     ESFQKALRGLEVGVDGLDEKTTDLDTITKEIGVPGPERIWYVGDAFRAGLSIDQVFAI
                     SKIDPWFLVQIKDIIDREQALKGKHIADLDKTALLQLKRRGFSDKRLATLLDTDQHAV
                     RAYRQALQVRPVYKRVDTCAAEFATNTAYMYSTYEEECEAQPTNNKKIMVLGGGPNRI
                     GQGIEFDYCCVHAAFAMREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLEDVLEIV
                     ALEKPVGVIVQYGGQTPLKLARGLEKAGVPIIGTTPDAIDKAEDRERFQKMLHELGLK
                     QPPNRTARAPEEAIRLAEEIGYPLVVRPSYVLGGRAMEIVQEQSQLERYMREAVKVSN
                     ESPVLLDRFLNDALEVDVDALCDNTGNVIIGGIMEHVEQAGVHSGDSACSLPPYSLSA
                     KLQDELRTQTKQMAKALGVVGLMNVQFAIQGTTVYVLEVNPRASRTVPFVSKACGLQL
                     AKIAARCMVGQSLASQKVTHEVIPPFYSVKEAVFPFIKFPGVDTILGPEMKSTGEVMG
                     VGETFAEAFVKSQLGASTKLPSGGKAFISVRKEDYNKVVEIAHALHDLGFTLVATKGT
                     ATALTANGLKVTSVNKVAEGRPHIVDMIKNNDISFIVNVTEDKRAVADSYEIRRSALQ
                     NKVTYYTTLAGAKAACMGMAHMQELTVESLQNLHKKLS"
     gene            15412..15894
                     /gene="greA"
                     /locus_tag="PKL42_00670"
     CDS             15412..15894
                     /gene="greA"
                     /locus_tag="PKL42_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNU65608.1"
                     /translation="MAVTQIPVTIKGAELLKEELQRLRAVDRPNIIQAIAEARAQGDL
                     SENAEYESAKERQSFIEGRISELEAKLSNLQVIDPQTLNADGRVVFGATVRFEDLDSG
                     DTKTYQIVGEDEADIKNGKVSVSSPIARGLIGKSEGEIAEVVAPGGVKEYEIIEVLYI
                     "
     gene            15894..16331
                     /locus_tag="PKL42_00675"
     CDS             15894..16331
                     /locus_tag="PKL42_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HNU65609.1"
                     /translation="MTYRIALIVVALWAGALWMTGVSAYVLFDTLQDKQLAGAIAGKL
                     FTVVSCIGMASGAFLITHRMAVFGTGALKQGYFWAVLLMLLLVLAGHFGIQPLLAQLK
                     ADALPLDVMQSVFVDRFRTWHGVASFAYIAECLLAIVMVLKIR"
     gene            16356..16706
                     /locus_tag="PKL42_00680"
     CDS             16356..16706
                     /locus_tag="PKL42_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNU65610.1"
                     /translation="MANSSLKIRVTHGNLIAMGPGKADLLEAIDRCGSISAAAKKMGM
                     SYRRAWELVDVMNKCFKQPLVSSSPGGPHGGGAQLTEFGYLILQTYRNLVAKANSSTA
                     AELELISTNLNPQV"
     gene            16818..18284
                     /locus_tag="PKL42_00685"
     CDS             16818..18284
                     /locus_tag="PKL42_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNU65611.1"
                     /translation="MKSPQPFSVSIFTAIATVIFPASGYALEDNEIARIKELEAQMIQ
                     LKQERVAQDKQLELLTNELIGIKNQVSQSNVAKAEEKGSSIGNLVYANFNDGLIFEEG
                     TGNWKLQLNGRVQADYRSYTPDAWKNDTFSIRRARLGGTFTFLKDFAARVEGEYANDN
                     AGAKGTTALTYGYLDFTRWPGAKIRAGQFKPFFGLERAYSTNFIDFTELSLATNNGSI
                     FTSTYDRGVMLFGDPLPWLNYNVYVVNGSGQNNDDVKDTKDSGLRVNANLAKLTKQEN
                     LVLHVGSSASQGSIGFSTSSGNGIAQATEASGIQFFNVVNILSTATTNRSRSGIEAAL
                     AYGPVKLQSEYISANFEGTKGVVKFDNDIKTWYVNLNWLVTGENYAEAYKSGVFGRIK
                     PKHNFDRKDGWGAFELGLRYSKFDASDFKPLLPAATANTSYTSEADAWTVGAKWLLNP
                     NARFLLNYIHTNFDTDIRVNGKLDDTEEAVALRAQYDF"
     gene            18332..19132
                     /gene="modA"
                     /locus_tag="PKL42_00690"
     CDS             18332..19132
                     /gene="modA"
                     /locus_tag="PKL42_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNU65612.1"
                     /translation="MQTIRNLLMKRKQLLLGILLAGLSTAAYAEKINIAAAADLKFAM
                     DEIVTRFKKGNPADEVEVIYGSSGKIFTQIQQAAPYDLYFSADISYPEKLEAAGLTAG
                     PTKPYAFGRIVLWSPKAQLDATKLRLEDISRAEISRIAIANPKHAPYGKRAEEALRSK
                     KLWHQVEKKLVYGENIAQTAQFVQTGNAQVGIIALSLALSAELSKQGGYWLIPESLHS
                     PLKQGFVITKRAETNQAAKKFSDYMSSAEARKIMVRYGFTLPDAVTAQ"
     gene            19161..19841
                     /gene="modB"
                     /locus_tag="PKL42_00695"
     CDS             19161..19841
                     /gene="modB"
                     /locus_tag="PKL42_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HNU65613.1"
                     /translation="MHLSPADIQAILLTLKLASVVTVILLLLATPLAWWLARTTSKGK
                     GVVSAVVALPLVLPPSVLGFYFLLAMGPQGPIGSLMHQLGWQALPFTFGGLVVASIIY
                     SLPFMVQPLQNAFEAVDKNLLDAAATLGASAQHRFFTIAVPLSRAGFLTAIILTFVHT
                     IGEFGVVLMIGGNIPGVTRVASVQIYDHVEALEYMQAHALSAVMLVFSFVVLLFIYTL
                     RPAHKSSH"
     gene            19872..20978
                     /gene="modC"
                     /locus_tag="PKL42_00700"
     CDS             19872..20978
                     /gene="modC"
                     /locus_tag="PKL42_00700"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HNU65614.1"
                     /translation="MKFHFYHHRQSFNLDMQAEIPDHGVMGVYGPSGAGKSTLLAMMA
                     GLVTPDAGQFTLEGACLFDRAKGINIPAHQRQIGMMFQDNRLFPQLTVQDNLNYGFKL
                     LAKEKRRLGFNQVVELLEIGQLLKQKPLQLSGGEKQRVALGRALLASPRLLLLDEPLA
                     SMDVRLKRQILPFLRRIKDETHTPMVYVSHAIDEILYLTPKIAMLEAGKILAVGDFNQ
                     VIHDERVLALAQSLGVENVLHTRILAHHATLGYTIATVGKQRIVMPPIDAPLGSMVSV
                     SIAAANIALSTHLIEGISIQNQLAGTVSSIQVVGSRTLVSVDVGVDLMVEVTNKALVD
                     LKIALGMQVYCLTKAQSIQPISMHEKPSEQKTLP"
     gene            complement(21064..21384)
                     /gene="yhbY"
                     /locus_tag="PKL42_00705"
     CDS             complement(21064..21384)
                     /gene="yhbY"
                     /locus_tag="PKL42_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HNU65615.1"
                     /translation="MKPTNLKLTTKQIAHLRGKAHDLNPVVMIGNNGLTDNVLKEIEL
                     NLNAHELIKVQVAGDDRDARKAIFNDIATKTNALAVHHIGKQLVFYRPSDTVKESAKI
                     VIPR"
     gene            21480..22097
                     /gene="rlmE"
                     /locus_tag="PKL42_00710"
     CDS             21480..22097
                     /gene="rlmE"
                     /locus_tag="PKL42_00710"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HNU65616.1"
                     /translation="MKPTKTSKAWMQEHLNDPYVKLAQKEGYRARAAYKLLEIDDKDK
                     LIKPGMTIVDLGSTPGSWSQVAVQRLKGQGRVIALDILDMHPIAGVEFIQGDFREASV
                     LRQLEEALNKSQVDLVIADMAPNITGIKDVDQAGAAYLTELALAFSKDWLKPNGNFLV
                     KVFVGAGFDELVKDMRQMFDKVVTRKPKASRDRSSEVYLLGLGKK"
     gene            22186..24081
                     /gene="ftsH"
                     /locus_tag="PKL42_00715"
     CDS             22186..24081
                     /gene="ftsH"
                     /locus_tag="PKL42_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNU65617.1"
                     /translation="MNNTFKNIAIWLAIVMVMLTIFNLTGVKNSTDNQVVYSQFMQEV
                     KAGQIAKVQIDGRVVRGTTHDGKKFSTYAPSDIWLVSDLLENNVVVEAKPDEEQSLFV
                     SILVSWFPMILLIGVWIFFMRQMQGGGKGGGPFSFGKSKARQLDETNNSTTFADVAGC
                     DEAKEEVTEIVDFLRDPTKFQKLGGRIPRGVLMVGPPGTGKTLLARAIAGEAKVPFFT
                     ISGSDFVEMFVGVGASRVRDMFENAKKSSPCIIFIDEIDAVGRSRGSGTGGGNDEREQ
                     TLNQLLVEMDGFEANSGVIVIAATNRADVLDKALLRPGRFDRQVMVGLPDIKGREQIL
                     LVHMRKVPIDVDVKADIIARGTPGFSGADLANLVNEAALFAARRNKRTVDMQDFEDAK
                     DKIFMGPERKSMVMREEERRNTAYHESGHAVVAKLLPKADPVHKVTIMPRGWALGLTW
                     QLPEFDRISNYKDKMLEEISILFGGRIAEEIFMHQMSTGASNDFERATKLARAMVTKY
                     GMSDALGTMVYAGDEQDSFFGSMSAKTISEATQQKVDAEIRRILDEQYALARKLLEDN
                     RDKVEAMTAALMEYETIDADQINDIMAGIPVRPPKPTQSKTSTPRDSGTTGVGAAPVP
                     QPSAKSE"
     gene            24155..25000
                     /gene="folP"
                     /locus_tag="PKL42_00720"
     CDS             24155..25000
                     /gene="folP"
                     /locus_tag="PKL42_00720"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNU65618.1"
                     /translation="MASPSESTSIFICGKYQLKLNRPHVMGIVNVTPDSFSDGGKYTS
                     TQAAVCHALTLIAEGADILDIGGESTRPGAAPVSLDEEISRVIPVIEALKGVSTVPLS
                     IDTYKPEVMRQAIAAGADMVNDVRALQEPGALDVLAKSHVGICLMHMQGNPMTMQMSP
                     HYEDVVIEVGQFLADRLQVCLNHGIAKNRIVLDPGFGFGKTREHNISLIQQLEKLHRI
                     GLPLLVGLSRKSVLGAIAGGDENQRLYASIAASVISAMKGAKIVRVHDVKATVDALKV
                     VAAIL"
     gene            25033..25305
                     /locus_tag="PKL42_00725"
     CDS             25033..25305
                     /locus_tag="PKL42_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65619.1"
                     /translation="MRTNDREIPAEAKDAIAEGKLIQAIKITRENTGLSLKESKERIE
                     AYLETDAVLKEKYLAHLQAKGLSQESVLRIAIIGFVAIIGYLIFFR"
     gene            25380..26735
                     /gene="glmM"
                     /locus_tag="PKL42_00730"
     CDS             25380..26735
                     /gene="glmM"
                     /locus_tag="PKL42_00730"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNU65620.1"
                     /translation="MSKKYFGTDGIRGRVGEYPITPEFVMRLGYAAGKVLTGLQVNLA
                     KGAHPAVLIGKDTRISGYMLEAALEAGLSAAGVDVLLTGPMPTPAVAYLTRALRAQAG
                     IVISASHNPYDDNGIKFFSADGAKLPDDIEHAIEAELDKPMQVMESAKLGKARRIDDA
                     AGRYVEFCKSTFPNHLDLRGLKIVLDCAHGATYHVAPDVFHELGAEIICIGNQPDGLN
                     INEQVGSTHPQALQKAVVEHQADLGIAFDGDGDRVMMVDAEGHLLDGDQLLYIIALGL
                     YAKGKLKGGVAGTLMTNLALEHALQKHQIPFGRANVGDRYVLELLNTKNWKLGGENSG
                     HILTLDKHTSGDAIIAALQVLQALVEGGKTLAQMREKLVLYPQVLINVTTKQKLDLNH
                     ASIQQAVKQAEVTLNNTGRVLLRASGTEPKIRVMVEGQDRTLVQKLAEDIAEVVKKAA
                     G"
     gene            complement(26947..27942)
                     /locus_tag="PKL42_00735"
     CDS             complement(26947..27942)
                     /locus_tag="PKL42_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNU65621.1"
                     /translation="MEHAIVIMAVLVVVALIFDFMNGFHDAANSIALMVSTRLLTPQA
                     AVLWAAFFNFIAFVIFGTAVAKMVGKGIIDPDIVNNAVIFGALNGAIIWNLITWWLGI
                     PSSSSHALVGGLVGAGVAKGGTGAIVAAGLIKTSAAIILSPLFGMLLAMILMVSVLWL
                     FEKSSPYPTERRFNKMQFVSSSLYSLGHGGNDAQKTMGIITVLLVANGFQQGFEVQFW
                     VIISCHIAMALGTLFGGWRIVRTMGMGITRIRPSGAFSAQTSGAIALFLATYLGIPVS
                     TTHTITGAIIGVGITRRASAVRWGLASRIVWAWVLTIPCSGLIAAAAYHFGKSFL"
     gene            complement(27952..28608)
                     /locus_tag="PKL42_00740"
     CDS             complement(27952..28608)
                     /locus_tag="PKL42_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNU65622.1"
                     /translation="MKSSIFSRLMSAITPHSDNYFILFNNLADCAVRASKVLAMMTAV
                     SEDEKFEVHFAEIRKIESEADGYTKEILLSLHKTFITPFDRREIRELAMALDDIIDFM
                     EDIPQSAKIYGRSSFTPEMVALAQILLRASEKVRDTVHMLGDMKNAERILRTCEEINL
                     IEGEGDHVMRAGVQRLFAEESDARTIMRSKVLYDLFEEAIDSCQDVSDVIHGVVLERI
                     "
     gene            28766..29818
                     /gene="arsB"
                     /locus_tag="PKL42_00745"
     CDS             28766..29818
                     /gene="arsB"
                     /locus_tag="PKL42_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006410676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNU65623.1"
                     /translation="MQPKSIATQKPQIGTFEKNLTWWVLACIVVGILLGQIFPDFFQA
                     VGSLKVAEVNLPVAVLIWLMIIPMLLKVDFGAMKEVLAHRRGISVTLFINWFVKPFSM
                     ALLAWIFIRHLFVAYLPAEQIDSYIAGLILLAAAPCTAMVFVWSNLCNGDAKFTLSQV
                     AINDAIMLVAFAPIVALLLGLSSIVVPWNTLLISVMLFIVVPVIISQILRKVLLAKSQ
                     LALETTLKQLHPISLTALLATLILLFGFQGQQILNQPMIIGLLAVPILIQTYFNSMLA
                     YWLNKKFKVAHCVAGPSALIGASNFFELAVATAIALFGFNSGAALATVVGVLIEVPVM
                     LSVVAIVNKSRAWYEA"
     gene            29840..30256
                     /gene="arsC"
                     /locus_tag="PKL42_00750"
     CDS             29840..30256
                     /gene="arsC"
                     /locus_tag="PKL42_00750"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148667.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HNU65624.1"
                     /translation="MPVTIYHNPACGTSRNTLAMIRASGVEPTVIEYLKTPPTREKLQ
                     EIISKTGGNIHALIREKGTPYAELGLDNPHLSEEALLDAMLQHPILINRPIVETSKGI
                     KLCRPSELVLDILDNANIGTFTKEDGEVVHAPSKSD"
     gene            30302..31406
                     /gene="prfB"
                     /locus_tag="PKL42_00755"
     CDS             join(30302..30382,30384..31406)
                     /gene="prfB"
                     /locus_tag="PKL42_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832626.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNU65625.1"
                     /translation="MEAEQLNLINNRLADLSERHLALRGYLDFENKSQKLEEVNGLLE
                     DPKIWDNAEKAQKLGKEKRSLELVVHNLQNLEAGIKDAQELFDMAREENDDDTLLSVK
                     ADSEALEKQIAEMEFQRMFSGELDNANCFVEFQSGSGGTEAQDWCNMLLRMYLRYTER
                     KGFKVEVLELSDGDVAGIKGASLKVTGDYAYGTLRTENGVHRLVRKSPFDSNAKRHTS
                     FASVQIFPEVDDSIQIEINPADLRTDTYRASGAGGQHINKTDSAVRITHIPTGVVVQC
                     QNDRSQHRNRDEAMNMLKGALYNLELNKRNAEKQALEDAKTDIGWGHQIRSYVLDQGR
                     IKDLRTGVEIGNTQGVLDGDLDPFIMESLKQGV"
     gene            complement(31455..32285)
                     /locus_tag="PKL42_00760"
     CDS             complement(31455..32285)
                     /locus_tag="PKL42_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNU65626.1"
                     /translation="MTTNQSTATNAVAAWVHFLSNKPIPVLKLTARELQLLKEDEDNL
                     TVRSITPIVLHDPFMVFRVLSYAKQHPSRHQQQDLIQVEQAILMMGTNAFYEKLPTTL
                     LIEDQLKDKLPALGQLLKIIKRTHRATYFATDWAAYHKDLRSDEVHIATLLHDLAEML
                     LWCFAPNKMLTIANLQQADKTLRSKAAQEQVLGFKISDLQKALVENFKLPPLLSKIMD
                     DENVTEHHAVNVRLAINLARHSANGWDDAALPDDYQEIAEFLRVDVEKAKVMIGVPTS
                     "
     gene            32446..33975
                     /gene="lysS"
                     /locus_tag="PKL42_00765"
     CDS             32446..33975
                     /gene="lysS"
                     /locus_tag="PKL42_00765"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008106791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNU65627.1"
                     /translation="MTDETPVIDENHVILERREKLKAIRETAKANQGAAFPNDFKPQH
                     KAADLHAQYGAFEKEALEPLAVQVVVAGRMMLKRVMGKASFATIQDGSGQTAGGRIQL
                     YIARDEIGEATYESFKHWDLGDILGATGKVFKTKTGELSIHVSEIKLLTKSLRPLPEK
                     FHGLADQEVKYRQRYVDLMTSEETRATFIARSKVVSAIRNFMIQNEFLEVETPMLHPI
                     PGGASAKPFITHHNALDMQMFMRIAPELYLKRLVVGGFERVFEINRNFRNEGLSVRHN
                     PEFTMMEFYAAYTDYQWLMDFTEQCIRAAAIAARGTAVLSYDGKEVDLSKPFERLTIV
                     EAIQKYAPEYSLAQLNDAQFLRADLYKRGIKVFEHAGLGSLQLSLFEETAESQLWQPT
                     YIIDYPVEVSPLARASDKNPEITERFELFITGREMANGFSELNDAEDQAARFHAQMKA
                     KEAGDQEAMYYDADFIRALEYGMPPAGGCGIGIDRLVMLITDSPSIRDVILFPHMRAE
                     N"
     gene            34256..>34761
                     /locus_tag="PKL42_00770"
     CDS             34256..>34761
                     /locus_tag="PKL42_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNU65628.1"
                     /translation="MNKKLGMAVIIAAAFTSGYASAADKVIKIDGSSTVYPVTEAVAE
                     EFQIATKNKVTVGESGTGAGFKKFCRGETDISNASRPIAQKEMDACKEAGIQFIELPI
                     AFDALTVVVNSKNDWVKSLSVEELKKMWEPKSSVKNWKQVNAAYPDKALALFGPGTAS
                     GTFDYFTEA"
BASE COUNT         9515 a         7280 c         8438 g         9428 t
ORIGIN      
        1 tgttacgatt atgacgattt cagtcacaga acgtaccaac gaaattggcc tgttggtcgc
       61 actcggcgcg cgccagcgaa cgattttggg gttatttctc agtgaatcgg tgattttggc
      121 tgtgctgggc ggcgcgatgg gtctgctatt tggcattggt cttgcccagc tattacacct
      181 gcttatgcca aactttcctg tacatacgcc ttggcgtttt gcggttgtcg ctttgctggt
      241 ttctggtgta attggtttaa ttgcgggggt attgcccgcc gtgagtgctt ctcgattaga
      301 cccgattgac gcattgcgcg cagaataagc tattatattg cccatgcgaa aacataaaaa
      361 aattcgtaac aaaaaaatcg gccttccacc tggttcgttg gtgtatgttg gcgatgctga
      421 catcaagcac ttagatacgc ttaaaatcac gcaaattact tatgatgccg atggcattgt
      481 tgaggctgaa ttagccatca gtgaattggc caattttaag caagacccaa acaaaaaaat
      541 atggctcaat gtacatggcg tgcatgatgt tgatgtgatt aaaaaactgg gggatttgtt
      601 taatctccac cctttggtga ttgaagatat tctcaatact gagcagcgcc ctaaaattga
      661 tgagtttgac gattatgttt ttttagaaac gcgtcatttt tattatcatc cagaaaattt
      721 atcagtaagt tcagagcaaa ttagcttagt gttgggtaaa tattttctgc tgacttttca
      781 agagcggtct acgggtgcat ttgccccgat tcgtgaacgt ctacgtgcca gtcattcaaa
      841 tatacgcgag caaggtgttg attatttagc ttatgcctta ttggatagtg tggtggatcg
      901 ctattttagt gtgctggaag ctgtcagtga cgccagtgaa acgcttgaag aagcgctatt
      961 ggccaaccca accaatgcag aactgcataa tattcaccaa ttaaagcacg ccagcattga
     1021 actgcgccgc gccgtgtggc ccttgcgtga ggttattaat agcctcaccc gtaatgaaaa
     1081 aggctttttt caaccaagta ccttgccgta tttacgcgat gtttatgacc atacagtcaa
     1141 ttttattgag tcgttggagt caatccgcga tagcttaagt ggcatgatgg atatttacat
     1201 ggccagtgtc agccagcggg tgaacttgga agtgcgcgcg ttaaccgtgg tggcgatgct
     1261 cttcatgccg gctaccctta ttgcgggaat ttttggcatg aactttcact ggatgccgtg
     1321 gattaacgac cgcaatgggt tttggtgggc attggcgatt atgtctggca ttgcgctcat
     1381 gatgattttg attttttggc gtcggcagtg gttaagtagt aaaacttaag ttttgatgag
     1441 ttgtgcatat tttgcgcgaa ttttagctgc ttttggcgcg gccactgcta tgcagtaagg
     1501 atttgctggg tttttatcaa agtaatattg gtgataatct tcggcagggt aaaaaatatc
     1561 tgcaccattg atttgcgtca caattggcgc accaaaacta tcagcctcgc tgaaggttgc
     1621 aatcatcttt ttcacagcat cttcttgcgc ttgattttgg caaaacaccg ctgagcgata
     1681 ttgtgtgcca atgtcattgc cctgacgatt gagcgtggtg gggtcgtgcg acacgaaaaa
     1741 cacttccagc aaagtttcaa aactgatttg gctggcatca aaggtgattt gaatcgcctc
     1801 ggcatgccct gtgtcaccgt tgcagatact tttgtacgtt ggattcatcg tgtgtccgcc
     1861 aatatagcca gacacgacgc tacttactcc atgaagctga ctaaagaccg gttcagtgca
     1921 ccaaaaacag ccgcctgcga atattgcaat ttgttggtta ctcatggtga gacccttttc
     1981 acttatttca aaatagtaat aaaaaaatca cgcacgcggg cgtatgatac ttagtcaatc
     2041 tttcatctaa aacattgcac tatttttaat atttcttggt tttaacattt tttagcatgg
     2101 cattaaacgc tttatacgtt gattttaact cctactttgc ctcagttgag cagcagcttg
     2161 tgccggagct gcgcggcaaa ccagtgggcg tggtggcggt aatggcagaa accacctgct
     2221 gtattgctgc cagttatgaa gccaaagcgt ttggtatcaa aacgggtacc ggcgtacgcg
     2281 acgcacgtaa attatgtcct gatatggtat ttgtgcaagc gcgaccgcca atttatgtgg
     2341 ccttgcatca tgaactcatt gaaattgtcg aaagttgtac gcatgttgaa aaagtgctgt
     2401 cgattgatga aatggtctgt aaactcaccg gcagccagca actgccagaa aacgcactca
     2461 agctggcggc caaaatcaaa cgcgcgatta gcaaaaaatt cgacacgatt cgctgctcta
     2521 ttggcattgc gcccaatact tttttagcca aaacggcatc aaacatgcaa aagccagatg
     2581 gctgtgtgtt gattgaacaa catgaattgc cgcaacgtct atttcactta aaactgcgtg
     2641 atttaaacgg catcggcaaa cagatggaag cgcggctcaa tcgctataaa atcaccacgg
     2701 ttgaacagct ttatgccgcc aatcgccagc aattacaaac cgcgtggggc agcattgaag
     2761 gcgagcgcat gtatgacaaa ctgcgcggcc tagagccata ttatgtgaaa aatgcacgca
     2821 gtagcttggg gcattcgcac gtaatgccac ccgagcagcg taatgaggcg ggtgtgcggg
     2881 cggtgttgca tcggctgtta caaaaagcct gtatgcgcat gcgaagttac gacttattgg
     2941 cttctaaaat cagcattaaa gtaaaattta gagataaacc aagttggaat atggaaagcg
     3001 cgatttcggc caccgacaat actttgcgcc tgattgaagc gcttgaaagc tttttacaac
     3061 actatcctaa aacccagcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa
     3181 aacccagcat gagccttatg cagtgggcgt taatttttca ggattggtga cggcagaaga
     3241 agtcgcgcgt gatttgtttc aagtggaacc gcttgaaaat gaaaaaaaac tcaaccaagc
     3301 cattgatgcg ctcaatttaa agtttggcaa aaataccctc tattttggtg gcgcacacga
     3361 tgcccttaaa gatgcgccaa tgcgcattgc ctttaatcat attcctgatt taaaagtgga
     3421 aggcgatacc tgattttgca tgcaaccttt tttgatgctc tggaagcctt ataaataaag
     3481 gctcgcaggg cataaatttt ttctcggcgt aaattgtggc aatttaaggc ttattgttgc
     3541 gtctgcaaaa ggctgttttt aaagtgcaag attgctagac attcagtacc gctgaatcaa
     3601 aaaaggctac aaaaatgtag ccttttttaa tcactcattg aaaagttgaa gaaccgtttt
     3661 tatattccct tagaatcttc tatttattta atatttattt aaaacctagc gccacggata
     3721 ttacatacct tgattgctgg attttcagcg gtcattaaat cagttcttgc aacgattttt
     3781 gtttcaagct ttgtagcaat taccgcacaa tcaatgcatt tgccttttgc tgtattaaac
     3841 cctttggtgg tttctaggcc tttaacatct ttaaatatgg catcatctac ttcggcatct
     3901 gttaagtttg ctccagtaag gtcagcccca gttaaatcag ctttaattaa atcagcgcgg
     3961 tataagtttg agttgcgtaa gttagcacca cgtagattac caaacctaaa tgcagccacg
     4021 cctaaattag cattttcaaa attgctatca gtgaagttgc cacccacgga atcaaatttc
     4081 atttgcccca tcggttgatt gcccaaatct agaccgagtt ggccgtttgt taaattggct
     4141 ttgtgtaaat caacattacc aagcgtacca ataaggcgtg catttgtcag gttggcttca
     4201 gcaaagttgg ttttatcgaa gattgcttga aaaatggtgg cattagttaa attagcttta
     4261 gagaagtttg cgttttctaa acgggcaaga ttcaaataag cgccttgaag attagcgcca
     4321 cttaaatttg cccccattag ttcagcgcca aaaaaactga tgttttgcat gttgcagtgg
     4381 ctcaaatcta agccattgaa aattaaataa ccagcatctt tgttgcttaa gtcgcaagtt
     4441 ttgctgttaa tttgtttgag tgcatcggct tggctaattt tatcgcgcat gggttctgca
     4501 ttttttgaat agaaagcaga agcttttgca attgtgttgt caggatctaa caaaaatgca
     4561 tctttcgcgg cctgattacc aaaacagtaa gttttacctt gataaatttc gttaatctca
     4621 catttggttt taacaagtga gcccttagat aagcctgttg tgcagtggtc accaaactca
     4681 cctgcgaagg caaaacttgt aaacgttaaa ccaatcacca aaaataattg tttgattctg
     4741 ttcatgataa ttctccaata atctgctcta gatttagagc cagtggtcac attattatcg
     4801 cggcagcagc attaatttgt aactcatgtc acatagttga aaataattat cattaagaac
     4861 tgtcgggcta agacacttct aaaattaacg gctaatgatt ttcatgagtt acaaattcgt
     4921 aacaactcct tgtcgccaaa accaaaaatg cgtgcgttat acgctcatct gcagaatatt
     4981 tcgcagagat tgaaaagtaa aaaattcaat ccaacgcgct gaagaagcgt aactaagttt
     5041 taacctgact taacaacttt aaaaagatta ggttaaataa aaggagaaat aaaatgactg
     5101 gttttaaatt aaatcttgtt aacaatatgc gtaccaaaat gttggtgccc gcacttttaa
     5161 cggctgtgtt tgcagcgcct gcgatggcaa atgaccaata tcgtgatact gcccgtgtgc
     5221 tttcagtcac accacaaact gagcgtgtga atgtgccacg ccaagagtgc cgcactgaat
     5281 atcagcaaca aagctacagc cacggtgcga acaacaatgt cactggggcg attatcggcg
     5341 gtgtagcggg tggcttgtta ggtagcacca tcggtcgcgg taacggccga gtagcggctg
     5401 cggcagtagg cgccggcgtg ggtgcgctgg ctggtaacag cattgctaac aacaacaatt
     5461 atcgtcatag cacacgcacc gtgccagtag aaagttgcta ccaagtagat aactggcaaa
     5521 ctgtgaatac cggttacttg gtaagttatg agtataacgg ccgcaattac accacagtaa
     5581 caaatgagca tccaggccgt tatattgatg tgaacgttgc ggtagcccct agaagccgtg
     5641 cggttacaca ggtgacttat gcacagccgc atgtgagcta cggtgattca cgctgggaag
     5701 acgatgacca tcgtcatcgc ggtcatcgtg gtaaacaccg tgattgggat cgtcgtggtg
     5761 atggccgtta ttactaagtt gagttaactt ttaactgtta aattaatcgt taaataagac
     5821 taaaaccagc tgcggattat gcagctggtt tttttatggg ccgatgatga cgttttaaaa
     5881 ttacgtatgt acttcatgca ggggtcagcg ttgaataatg ttaaaatcgc gcatgcccaa
     5941 ttttatgcaa caaagcgttg aattttttaa gcgattattt ttaaaaaaaa cgcgacctaa
     6001 cgccgctgtt gcttggcagc aattgggtca tgacaccgca ggagcttatt ggctttacgc
     6061 cgccccagta cacttggtgt tgcagcgtga tacctttagt ttggcggccc ccgtgccttt
     6121 gccattagag cagcctgaaa ttgatacgct gaccaccttg ttgaacacgc attttggttc
     6181 agatggcata cagttttatt ggcacgaaaa tctttggttt ttaaggttag cacaacatcc
     6241 acagattcaa acctctttac cagaaagcgc gctcaatcaa gatattcatg cctttatgcc
     6301 cacgggcgaa ggggcgatgc gctgggcgca ggttgtcaat gaaatgcaaa tgctgttatt
     6361 tgagcaccct gtgaataacg cgcgtgaagc aaggcatctg ccggccatga atagtctttg
     6421 gtgctacggc ggcggccaat tgccgcaatc gccaaaataa gcaatcctta tgcaaatcaa
     6481 accacgcact tttgatgaaa atattgttag cacgctgatt gcagaaggcg tcaatcctct
     6541 gttggcaaag ctctttgcgg cgcgcggtgt tgccaataaa cacgcgcttg aaacctcgtt
     6601 gggccaaatc attccgccca cgttactcac taataatacg gccatggcca aattgttggc
     6661 cgatgccatt gcgcaaaata aacatctgtt agtcattggc gattacgatg cagacggtgc
     6721 gactgcgacc gccgttgctg taaaaggctt gcgcgctttt ggtgcgcggg tagatttttt
     6781 agtgcccaat cgttttgaat acggttacgg cttaacacct gaaattgtgg cgcttgccgc
     6841 ccagcaaaaa ccggatgtca ttattacggt ggataacggc attgccagtg tagaaggtgt
     6901 ggccgcagcc aatgcgctgg gcatgcaagt gctgattacc gaccaccact tgcccggcca
     6961 aatcacgccg caggccgcct gcattattaa cccaaatcag catggctgtg attttccgag
     7021 taagcacttg gccggcgtgg gcgtgatgtt ttatggcttg ctggcgttgc gtgcagaatt
     7081 acgtgcgcgt ggcgctttta cgctgacgcc tgaacccaat ttaactgaat tgctggattt
     7141 agtcgcactc ggcacagtgg ctgacttggt gaagctcgat gacaacaacc gcattttggt
     7201 ggaacaaggc ttgcgtagaa ttcgtgcagg cgcgtgttct ctaggcataa gcgcgctgtt
     7261 gcgcgtgagt ggtcgccagc cacaacaagt gaacgcgcaa gatttaggct tttatgttgg
     7321 ccctaggctc aatgctgccg gcaggctgga tgatatgcgc ttgggcattc aatgtttgtt
     7381 ggccgaaact gaagttgagg ctgcaaccat cgcccagcaa ctgcatacgc ttaatctgca
     7441 acgccgcagt attgaggccg atatgcaaga gggcgcgcaa ctctcgttag acgacattgc
     7501 ggttgaaagc caatttagca tcagtattta tcaagatgat tggcaccagg gcgtgatagg
     7561 tattttggcc tcgcggatta aagagcgcca tcatcggccc gtcattgcct ttgccaatgc
     7621 gggtgacggt ttgctaaagg gttcagggcg ctcaattgca gggctacatc tgcgagatgc
     7681 cttggatttg cttgccaaac atcagcccga tttgatttta aaatttggtg gccacgccat
     7741 ggcagccgga ttgaccataa agcttgttga ttttgaacgg tttaatcata gctttgaggc
     7801 ggtggtcaaa agtttgatta ccgaagcaga tttagcgtcg gtgctagaag ttgatggcag
     7861 tttagcggcc gatgtcatga cgtttgaaac cgcgcaagcg ctcgaaaacc aagtttgggg
     7921 gcaaggcttt gcaccgccac tgttttttga ttgctttgaa gtggtcagcc agcgcgtgtt
     7981 gagtgaaaaa cacctcaagc tcactttaaa aaaagaaaat gccctgattg aagccattta
     8041 ctttaattgc gcagaagcat ttgctggcgc aatttttgcc gcctatcaat tgcaaacaaa
     8101 tagttataac ggcgcgcaaa aagtgcagtt aaatttaaaa tatgcaacct gtgaaaaacc
     8161 gctttttgca agctaatttt acaccaacct tttttgatgc cctggaagcc ttataaataa
     8221 aggctcgcag ggcataaaat ttttctcgta caaaagcggg ctgttttaaa cgcttttttt
     8281 tgcacgagat tagagcaaaa aaatcacaaa aatttaggcg gaatacgagt aagatatagc
     8341 aaacacttga gcaatttttt taaatttgaa aggttcacca tgcaaactag aatatgcaca
     8401 aaacaaccat tgttaaaagt tacgctattg tcagcggtgc tgctggctgt attgggcgca
     8461 ggttgcgcaa aagaagttaa agccccgcaa caggcagccg ttgagcggca aaaaatcacc
     8521 ggtttaaaaa cgccagaatc tgtggtgcag gccaaagatg ggcggatttt tgtgtctgaa
     8581 atcggtgaat ttggtaaggc tggggatggc gtaatcacgc taattgacgc gcaaggtaaa
     8641 acttcagtgt atgccagcgg cttagatgac ccgaaagggt tagccgtgat tggaagtgat
     8701 ttgtatgtgg ctgataatca taaggtttta aagattacct cagatggaaa atccagcgta
     8761 tttgcaagtg cagaagcgtt ttcaggcgcg ctgcatggct caccttattt tttgaatgac
     8821 ttagaagccg acccacaagg caatttatat gtgagtgaca gcggtgattt gttcaacacg
     8881 ggcttgggtg gtgccatttt caaaatcaat ccgcaaggcc aagtaagctt attattgagc
     8941 aataagcaag atgtgcgcat taaagcgccc aacggcttgc tggcggatga tactggcgaa
     9001 gtgttgctgg tggtggattt tagttcaggt attttgtata gcttgaacca aaaaacccaa
     9061 gtattgactg accttgcaga agggtttggc ggaggcgatg gtttggtgca tcactctgat
     9121 ggcaccatgt acgtgagcga ttggaaatca ggcaaagtgt ttagcgtgag caccaaaggc
     9181 gatgtcacgg aaattaaaag cggttaccaa gccgctgcgg atattgccat caccaaggac
     9241 gaaaaatacc tgatggtgcc tgatatgaaa gcgggcgaat tggactttat tctgcttaaa
     9301 taaggggtta tgtaggtgcg caatcaattg cgctcctaca tgttttaacc atgaacgaac
     9361 tacgtcaaaa cgctttacat tggcttgctt gcagccaagt ggccgataag gttgcgggcg
     9421 ttaccgcgct caatcttgct tggcaagcgg gcgatgtatg cttggatgca agcatacagc
     9481 ttactgcaaa tcaggcgatt ccgggccgcc cggccaaacc cgttttagtt tcaccgttag
     9541 ccgttgaaaa gcgttccatg cgcacccttg acggtcgcgc ggcactgatt cacgcgattg
     9601 cgcatattga gtttaatgcc attaacttgg cacttgatgc cgtttggcga tttaccgaca
     9661 tgccaaaaac ttactatgcc aattggctga aagttgctgc cgaagaggct tatcacttta
     9721 atttattgca ctcgcatttg cagcaaatgg gttatgggta tggcgatttt agtgggcata
     9781 atagtttatg ggagatggcc gagcgtacta atgacgatgt gctggcgcgc atggctttag
     9841 tgccgcgcac tatggaggcg cgtggactgg acgcctctgt gccattacgc aataaatttg
     9901 cacaaattgg cgatacggct gttgcggata ttctggatat tattctacgc gatgaaattg
     9961 gccatgtggc cattggcaac cattggttta actggctgtg tgcggcgcgt caattgtcag
    10021 ccattgcaac gtttgagcaa ctttgccagc aatataaggc acctaaactg cgcgggcctt
    10081 ttaaccttga ggcgcgtcgg ttggcaggat ttagtgaggc tgaattggct ttgttaggct
    10141 gattgatgtt aaaaacctta aagatttcaa tattcaaatg acgactaatc accgactatt
    10201 ttttcctatt ttacttttga tcttggtggc agtgtttgtc tggtcagcca tgcaaccggc
    10261 cgattatttg acttggtttc ttgaagttgc ccccgtgctg attgccttgc caatcttgtg
    10321 ctttagttat caaaagtttc cgctaacgcc attgctgtac gtgttaattt tggtacatgc
    10381 tgtcattttg atgataggcg ggcactatac ctatgctgaa gtgccagctt ttaactggct
    10441 acgcgacact tttgaactga gtcgcaatta ttacgatcgt gtgggtcatt ttgcacaagg
    10501 gtttattcct gcgatggtgg cgcgtgaaat attgcttcgc aaaaatgttg tacaaaaagg
    10561 cgcgtggctg ttttttatcg tgtgctgtat tgccttgagt atcagtgctg tttatgagtt
    10621 gatagaatgg tgggtggcgg tggcttcagg caaagcggca gaggcttttc tgggcacgca
    10681 aggtgacgtg tgggatacgc aatgggatat gttcattgcg cttattggcg cggtaattgc
    10741 acaattagta ctggcaaatt cccacgaccg acagctacaa accctgtaat gtcagcttga
    10801 taagccgtta gtttttcgct ataattcggt aattctgaaa cgggaagagt ctctatacac
    10861 tcctcccgtt ttgcatatct gccatctgct catgatggac aaaaagttaa aattaattaa
    10921 tgttgtaaaa caaggagttg ccgtgtcaca aaacctgccc gccatactag tacttgcaga
    10981 tggaactgtg tttcgtggca cctcaattgg ggcaagtggc caaaccgttg gtgaggtggt
    11041 gttcaacact tcaatgactg gttatcagga gattttaacc gacccctcct acaccaaaca
    11101 aatcgtcacc cttacttatc cgcacatcgg taattatggc gttaaccttg aagatgttga
    11161 gtctggccaa gtgtatgcca gtggccttat tattcgtgat ttgccattaa ttcactccaa
    11221 ttttagaaac acacagtcat tatctgacta tctggttgat aacaatattg tcgcaatcgc
    11281 cgatattgat acacgcaaac ttacccgaat tttacgtgaa aaaggtgcgc aaacaggtgc
    11341 ggtaatcgca ggtgaggctg atgaaacgaa agcgttagca ttggcgaatg atttgatagc
    11401 gggctttcca ggtttaaatg ggctggattt agcaaaagtt gtgacctgcg aaaaaccata
    11461 cgaattcact gaaggtgagt gggcgttagg tcaaggattt acgccagcgc cagccaagca
    11521 gtttcatgtg gtggcttacg attacggggt taagcgcaat attttgcgga tgctggtttc
    11581 tcgtggctgt aaggtcacgg tgttgccagc ccaaacatcg gcggctgagg cgcttgccta
    11641 caaaccagac ggtgtatttt tatccaatgg tccgggggac ccggcaccgt gtgattacgc
    11701 aattgctgca attaaaacca ttgtcgatag cggtgtgcct acgtttggca tctgtttagg
    11761 gcatcagttg ttggcattgg cgtcaggggc taaaacaatc aaaatgaaat ttggccatca
    11821 tggtgctaat caccctgtgc aagatgttga aaccaagcgc gtgtatatca ccagtcaaaa
    11881 ccatggcttt gcggcggatg cgaccacatt gccctccaat attaaaacca cccatgtttc
    11941 attgtttgac ggcagtttgc agggcattgc ccgtactgac aaacccgcat tcagctttca
    12001 gggccatcct gaagccagtc cggggccaac cgaaatgagt tatttgttcg accgcttcat
    12061 tagcatgatg caggcaagta aaacagcaga aagaaaggct agctaatggc taagcgtacc
    12121 gatataaaat ccattcttat cattggcgca ggtccaattg tcattggtca ggcctgtgag
    12181 tttgactact ctggcgcgca ggcatgtaaa gcgttgcgtg aagaaggtta ccgcgttatt
    12241 ttggtgaact caaacccagc caccatcatg actgatccgg aaatggccga tgcaacctac
    12301 attgagcctg tgacttggca agtggttgaa aagattattg ctaaagagcg ccccgatgcc
    12361 ctattgccaa ccatgggtgg tcaaactgcc ttgaattgcg cactcgattt agataagcat
    12421 ggcgtgttgg caaaatataa ggtggagttg attggcgcat caaaagaggc gattgataaa
    12481 gccgaagacc gccaaaaatt caaggaagcg atgaccaaaa ttggtttagg ttccgcgcgt
    12541 tctgccatcg cgcatagcat ggaagaagcg ttgcaggtac aagccagtat cggttaccca
    12601 gcgattattc gcccatcatt caccatgggc ggtagcggtg gcggcattgc ttataaccgt
    12661 gaagaattta tcacaatatg tgagcgtggg ttggatgcct caccgacaaa agaattgttg
    12721 attgaagagt cattgctcgg ctggaaagaa tatgaaatgg aagtggtacg cgactctgcg
    12781 gacaactgca ttattatctg ttccattgaa aatttagacc caatgggcgt acatacaggc
    12841 gacagcatta ctgttgcgcc tgctcaaacc ttgactgaca aagaatatca aatcatgcgt
    12901 aatgcctcat tggccgtatt gcgtgaaatt ggtgtggata cgggcggctc taacgtgcag
    12961 tttgccatca acccggatga tggtcgtatg attgtgattg agatgaaccc acgcgtgtcg
    13021 cgttcatccg cgctggcctc taaggcaaca gggtttccaa ttgcgaaagt ggcagcaaaa
    13081 ttggctgttg gttttacgtt ggatgaactg cgcaatgaaa ttaccggcgg cgcaacaccg
    13141 gcctcattcg agccaagtat tgactacgtc gtgactaaaa tcccacgttt cgcattcgaa
    13201 aaattcccgc aggcagatag tcgtttaacc acacaaatga aatctgtggg cgaagtgatg
    13261 gcgattggcc gcacgttcca agaatcattc caaaaagcct tacgtggact agaagttggc
    13321 gtggatggtc tggacgaaaa aactacagat ttagatacca ttaccaaaga aattggcgta
    13381 cctggccccg agcggatttg gtatgtgggc gatgccttca gggcaggttt aagtattgat
    13441 caggtctttg cgatttcaaa aattgaccct tggtttttgg tgcaaatcaa agacattatc
    13501 gaccgtgaac aagcgcttaa aggcaagcat attgctgatt tagacaaaac agcattgttg
    13561 cagcttaaac gccgtggttt ttctgataag cgtttggcaa cattgctcga taccgaccag
    13621 cacgctgtgc gtgcttatcg tcaggctttg caagtgcgcc ctgtttacaa acgtgtggat
    13681 acgtgtgcgg cagagtttgc tacgaatacc gcgtatatgt actctaccta tgaagaagag
    13741 tgtgaagcgc agcccaccaa taataaaaaa attatggtgt tgggcggtgg cccaaaccgt
    13801 attgggcaag gcattgagtt tgattattgc tgcgtacatg cagcctttgc gatgcgcgaa
    13861 gatggttatg aaaccataat ggtgaactgc aaccctgaaa cggtttcaac cgattacgac
    13921 acctcagacc gcttgtattt tgagcctgtg acattagagg atgtgctgga aatcgtagcg
    13981 cttgaaaaac ctgttggcgt gattgtgcaa tacggcggcc aaacgccact caagctcgcg
    14041 cgcggcttag aaaaagcagg tgtgccgatt attggcacga cgccagatgc cattgataaa
    14101 gccgaagacc gtgaacgttt tcaaaaaatg ttgcatgaat tgggcttaaa gcaaccgcca
    14161 aaccgcaccg cacgcgcgcc agaagaagct atccgtttgg cggaagagat tggttaccca
    14221 ctcgtggtac gcccttccta tgttttaggc ggtcgtgcaa tggaaatcgt gcaagagcaa
    14281 agccagcttg agcgttacat gcgtgaagca gtcaaagtat caaatgaatc gccagtattg
    14341 ctagataggt ttttaaatga cgcgcttgaa gtggatgtgg atgcgttatg cgacaacacc
    14401 ggcaatgtga ttattggcgg tattatggaa cacgtggagc aagcaggtgt tcactctggt
    14461 gactcagcct gttcactccc tccatatagt ctgtcagcca aattacaaga tgaattacgt
    14521 acgcaaacca agcaaatggc caaagctctg ggcgtggtgg gtttgatgaa tgtgcaattt
    14581 gcgatacagg gtactaccgt ttatgtgtta gaagtgaatc cgcgggcctc acgtaccgta
    14641 ccgtttgttt caaaagcctg tggtttgcaa ttagcaaaaa ttgccgcacg ctgtatggtt
    14701 ggccaaagtt tagcttctca aaaagtcaca catgaagtta ttcccccatt ctactctgtg
    14761 aaagaagccg tgtttccgtt tattaaattc ccgggtgtgg ataccatttt aggcccggaa
    14821 atgaaatcaa ccggtgaagt gatgggggta ggtgagacct ttgctgaagc ctttgtgaag
    14881 tcacaactag gtgcttccac caaattacct tcaggtggta aggcgtttat aagtgtgcgt
    14941 aaagaagact acaataaggt ggttgaaatt gcgcatgccc tacatgattt aggttttaca
    15001 ttggttgcca ctaaaggcac agctacagca ctcacagcca atggccttaa agttacctct
    15061 gtgaataaag tggctgaagg gcgcccgcat attgttgata tgataaaaaa caatgacatt
    15121 agttttattg tcaatgtgac tgaagacaag cgcgcagttg cggatagtta cgaaattcgt
    15181 cgtagtgcgt tgcaaaataa agtgacttat tacacaacgc ttgcgggcgc aaaggcagcc
    15241 tgcatgggca tggcgcatat gcaggagctg acagtggagt cacttcaaaa cttgcataaa
    15301 aagctgtctt aaaatacact tcaacaatcg tatttaaatt taatttgttc aaccacactc
    15361 tcgcgggtgt ggtttatttt ttatgcttta atttttacaa ggtttagcaa tatggcagtt
    15421 acgcaaattc ccgttaccat caaaggtgcc gagttactca aagaagaatt acaacgctta
    15481 cgtgctgttg atcgccccaa tattattcaa gcaattgcgg aggctcgtgc gcaaggcgat
    15541 ttgtcagaaa atgctgagta tgaatctgca aaagagcgtc agagctttat cgaaggccgt
    15601 atttctgaat tagaagcaaa gctttctaac ctgcaagtca tcgacccgca aaccctcaat
    15661 gcagatggcc gcgtggtgtt tggtgccact gtgcgttttg aagatttaga ttcaggcgat
    15721 actaaaacct accaaatcgt tggtgaagac gaagcggata ttaaaaatgg aaaagtgtct
    15781 gtgagttcac ctatcgcgcg cggattaatt ggcaaatcag aaggtgagat tgctgaagtc
    15841 gtcgcacctg gcggtgtaaa agagtacgaa atcattgaag tgttgtacat ctaatgacct
    15901 accgcatcgc cctcattgtc gtcgcccttt gggcgggggc gctctggatg acgggcgtca
    15961 gcgcctacgt gctatttgat accctgcaag acaagcaact cgcaggcgca attgccggaa
    16021 aattattcac cgtggtgagc tgtattggca tggcgagtgg cgcgtttcta ataactcacc
    16081 gcatggcagt gtttggtact ggcgcactaa aacaaggata tttttgggca gtgctactga
    16141 tgctgctact agtgctggca ggccactttg gtattcaacc tttacttgca cagcttaagg
    16201 ccgacgcctt acctttggat gtgatgcaaa gtgtctttgt agaccggttt agaacttggc
    16261 atggggttgc cagctttgct tatattgcag agtgtttgtt ggcgatagtg atggtgttaa
    16321 agatacgtta gatatatcgt gcagcgcatt atttgatggc taatagcagt ttaaaaataa
    16381 gggtaacgca cggaaattta atcgccatgg ggccaggtaa agcggattta ctagaagcga
    16441 ttgatcgctg tggctctatt tcggccgcag caaaaaaaat gggtatgtca tatcgacgag
    16501 cttgggaact ggtggatgtg atgaacaaat gtttcaagca gccattagtg agctcatcac
    16561 cgggcggacc acacggtggt ggtgcgcaat taactgaatt cggctatcta atacttcaaa
    16621 cctatcgtaa tttagttgct aaggctaata gctcaaccgc tgcagagctt gaactaatca
    16681 gcactaattt aaatcctcag gtttaattct taccaaaaat tctcaagtct tttctatcgc
    16741 tattcccatt caaatataac gcatgttatg attagccttt agcgtctatg atattttaat
    16801 tcacgataga gtgattgatg aaatccccac aaccattttc agtatcaata tttacggcaa
    16861 ttgctactgt tatttttccg gcgtctggat atgccctcga agacaatgaa attgccagaa
    16921 taaaagaact cgaagcacaa atgatacaac tcaagcagga acgagttgcg caggataagc
    16981 aacttgaatt gttgactaat gaactgattg gtatcaaaaa tcaagtgagc caatctaatg
    17041 ttgcaaaagc tgaagaaaaa ggttcaagta taggcaatct agtctatgct aactttaatg
    17101 acggtcttat ttttgaagag ggcactggaa attggaagtt acaattgaat gggcgcgtac
    17161 aggcagatta cagaagctac acaccagatg cttggaagaa tgacaccttc agcatacgcc
    17221 gcgctaggct tggcggcact tttacattcc ttaaagattt tgctgcgcgc gttgaaggcg
    17281 agtacgccaa tgataatgcc ggcgcaaaag gcacaactgc actgacttat ggttatttag
    17341 actttacgcg ctggccaggc gctaaaataa gagcaggtca atttaaacca ttttttggct
    17401 tagagcgtgc ttatagcacc aatttcatcg attttacaga actttcactt gccactaata
    17461 atggcagtat tttcacatca acttatgatc gcggtgtcat gctgtttggt gacccgttgc
    17521 catggctaaa ttacaatgtt tatgtagtga atggtagcgg tcaaaataac gatgatgtaa
    17581 aagataccaa agatagcggc ttaagagtca atgcaaactt agcaaaatta accaaacaag
    17641 aaaacttagt cttacatgta ggctcatcgg caagtcaagg cagtattggg ttctcaacat
    17701 cttcaggcaa cggcattgct caagccacag aagctagtgg tatacaattt ttcaatgtcg
    17761 taaatatact tagcaccgca accacaaata gaagccgaag cggcattgag gctgccttgg
    17821 cttatggccc agttaaactt caatcggaat atatcagtgc gaattttgaa ggtacaaaag
    17881 gggttgttaa gtttgataac gacattaaaa cttggtatgt gaatctgaat tggctggtca
    17941 caggtgaaaa ttatgctgaa gcttataaaa gtggtgtgtt tggccgcatt aaaccaaaac
    18001 ataactttga tcgtaaagat ggttggggcg cgtttgagtt aggactgcgc tacagtaaat
    18061 ttgacgcgtc tgattttaaa ccattattgc ctgctgctac tgcaaacact tcttatacta
    18121 gcgaagcaga cgcttggaca gttggtgcta agtggttgtt aaaccccaat gcccgttttc
    18181 tattaaatta tatacacacc aattttgaca ctgacattcg tgttaatgga aaattagatg
    18241 atactgagga agccgtcgcg ttgagagcgc aatatgattt ttaaccccat tatttttaac
    18301 ccatgattat ttcaaatttt aggaaaatca aatgcagacc attagaaatt tgttgatgaa
    18361 acgtaaacag ttattactcg gaattttact ggcaggtcta tcgactgccg cctatgcaga
    18421 aaaaatcaac attgccgctg ccgcagattt aaaatttgcc atggacgaaa ttgtcacgcg
    18481 gtttaaaaag ggcaatccag cagatgaagt ggaggtgatt tacggttcat caggtaaaat
    18541 ttttacacaa attcagcaag cggcccctta tgatttatat ttttcagcag atattagcta
    18601 ccccgagaaa ttagaagcgg ctggccttac agcaggccca actaagcctt acgcgtttgg
    18661 acgtattgtt ttatggagcc caaaagccca gttagatgca actaaattgc gcttggaaga
    18721 tatctctcgc gcagaaatta gccgcattgc cattgccaac cccaaacatg cgccttatgg
    18781 caagcgtgct gaagaagcgt taaggtcaaa aaaactttgg catcaagttg agaaaaagtt
    18841 ggtttatggt gaaaatattg cgcaaaccgc gcagtttgtt caaactggga atgcacaagt
    18901 agggattatt gcgctatcac ttgcattaag tgccgaactc tcaaaacagg gtggctactg
    18961 gctaattcct gaatctttac atagtccgct taagcaaggg tttgtcatca caaaacgtgc
    19021 agaaaccaac caagccgcta aaaagttttc tgactatatg tcaagtgctg aggcgcgaaa
    19081 aatcatggtg aggtacggtt ttactttgcc tgatgcagtg acggctcaat aaacaattgt
    19141 tatcaattta ataaaatacc atgcatttaa gccccgccga tattcaagcc atattgttaa
    19201 cacttaaact cgcaagtgtc gtcaccgtta ttttgctgtt gttggcaacg ccgcttgctt
    19261 ggtggcttgc acgcaccacc tcaaaaggca aaggggtggt gagtgcagtg gtcgcgttgc
    19321 ctttggtgtt gccgccttct gtgcttgggt tttatttttt attggctatg gggccacaag
    19381 gacccattgg cagcttgatg catcagttgg gctggcaagc gttaccgttc acttttgggg
    19441 ggttagtggt ggcatctatt atctattctt tgccatttat ggtgcaaccc ttgcaaaatg
    19501 cgtttgaagc agtggataaa aatttactgg atgccgctgc gacgcttggc gcttctgcgc
    19561 agcatcgttt ttttacaatt gctgtgccgt tgtcgcgtgc tgggttttta acggccatta
    19621 ttcttacctt tgtacatact atcggtgaat ttggcgttgt attaatgatt ggcggcaaca
    19681 ttccaggcgt gacgcgcgtg gcttctgtgc aaatatacga ccacgtagaa gctcttgaat
    19741 atatgcaagc gcatgcgctt tcggcagtga tgctagtctt ttcatttgtg gtgttattgt
    19801 ttatttatac gctgcggcca gcgcataaaa gtagtcacta attactagaa accgccaaac
    19861 aacaattacc catgaaattt catttctatc accaccggca atcttttaat ttggacatgc
    19921 aggctgaaat tccagaccac ggtgttatgg gtgtttatgg cccatcgggt gctggaaaaa
    19981 gcaccctgtt ggcgatgatg gccggccttg ttacgccaga tgcgggacaa tttaccttag
    20041 aaggtgcgtg tttgtttgat cgtgcaaaag gcatcaacat ccccgcacat cagcgccaga
    20101 ttgggatgat gtttcaagat aatcggctat ttccacagtt aacagtgcaa gataatttga
    20161 attatggctt taagttacta gctaaagaaa agcgtcgttt aggtttcaat caggtggtcg
    20221 aactactgga gattggacaa cttttaaagc aaaagccatt gcaactctca ggcggcgaga
    20281 aacaacgcgt ggcgttaggc cgggcgttgc tggcatcgcc acgtttgttg ttgttagatg
    20341 aaccgcttgc ctcaatggac gtgcgcctga aacgtcaaat cctaccgttt ttacgccgaa
    20401 tcaaagatga aacacacacg ccgatggtgt atgtcagcca tgccattgac gaaattttgt
    20461 atttaacgcc aaaaattgcc atgctagagg ctggaaaaat tctggcagta ggcgacttta
    20521 atcaagtgat tcacgatgag cgcgtgctgg cactcgcgca atcgctcggg gtagaaaatg
    20581 tcctgcatac ccgcatttta gcgcatcatg caacgcttgg ttacaccatc gctaccgtcg
    20641 gcaagcagcg tattgtgatg ccacccattg acgcgccgct gggtagcatg gtttcggttt
    20701 cgattgccgc tgctaatatt gctttatcca cccatttaat tgaggggatt tcgatacaaa
    20761 accagcttgc aggtacggtt tcaagcattc aggtggttgg gtcaagaaca ttggtatcgg
    20821 tggatgttgg cgttgatttg atggtagaag tcacaaataa agcgttggtt gatttaaaaa
    20881 ttgcgctggg gatgcaggtg tattgcttaa ctaaagcgca gagcattcaa cctatttcaa
    20941 tgcatgaaaa accctccgag caaaaaacac tgccctgaga gccttataaa taaaggcttc
    21001 tagggcagtg caaaagggtt gtgcttaaat aatgctaaaa aaagtaattt ttaacgcgct
    21061 aaattatctt ggaatgacaa ttttggcaga ctcttttacc gtgtcgcttg ggcgataaaa
    21121 gaccaactgc ttgccaatgt ggtgcactgc aagcgcattg gttttagtgg caatgtcatt
    21181 aaaaatagct ttgcgcgcat cgcggtcgtc gcccgctact tgcactttaa tgagttcgtg
    21241 cgcattcaaa ttcagctcaa tttcttttag cacgttgtca gtcaggccgt tgttgccaat
    21301 catcaccaca gggttcaagt catgtgcttt gccgcgtaag tgtgcaattt gtttggtggt
    21361 gagctttaag ttagttggtt tcatgatgtt cttttataat gtgatgactg ctgcacattt
    21421 taacatgaag ccatgtttaa cgttgaatga aaccttatct cgaatgaagt cgatctgcta
    21481 tgaaacccac taaaaccagc aaagcgtgga tgcaagaaca tttgaatgac ccctacgtaa
    21541 agcttgcgca aaaagagggt tatcgcgcgc gcgcagctta taaattgctc gaaattgatg
    21601 acaaagacaa actcatcaag ccgggcatga cgattgttga tttaggctca acgccaggta
    21661 gttggtcgca agtggcggtg cagcgtctaa aagggcaggg acgtgttatt gcactcgata
    21721 ttctagacat gcatccgatt gcgggtgtag agttcattca aggtgatttt cgtgaagcat
    21781 ccgtattgcg gcagcttgaa gaggcattaa ataaaagtca ggttgacctt gtaattgctg
    21841 acatggcgcc caatataacc ggtatcaaag atgtggacca ggcaggtgcg gcctatttaa
    21901 ctgagttggc gctggcgttt agcaaagatt ggctgaaacc taatggcaac tttctagtca
    21961 aagtgttcgt aggcgctggt tttgatgaac ttgtcaaaga catgcgacaa atgtttgata
    22021 aagttgtgac acgcaaaccc aaagcctccc gcgaccgtag cagtgaagtg tatttgcttg
    22081 gtttaggaaa aaagtagtaa aaacaggttt ttcagcattt tcatcagatt aaaaatgccg
    22141 taaaatcaga cagatattta tcccttttaa taaaggaacg gaattttgaa taatacattt
    22201 aagaatattg cgatttggtt ggcgatagtg atggtcatgt tgaccatatt taatctaaca
    22261 ggcgttaaaa acagcactga taaccaagtt gtgtattcac aatttatgca agaagttaaa
    22321 gctggccaaa ttgctaaagt gcaaattgat ggacgtgttg tgcgtggcac aacccatgac
    22381 ggtaaaaaat tcagcactta tgcgccttca gacatttggc tggtttcaga tttgcttgaa
    22441 aataacgtgg tggtagaagc taaacctgat gaagagcagt cattgttcgt gagcatactc
    22501 gtgtcatggt tcccaatgat tttactcatt ggcgtttgga ttttctttat gcgccaaatg
    22561 caaggcggcg gtaaaggtgg tgggccattt tcttttggta agagcaaagc acgccagtta
    22621 gacgaaacca ataactccac gacttttgct gatgtggccg ggtgtgacga ggcaaaagaa
    22681 gaagtgactg aaatcgtcga ctttttgcgt gacccgacca aatttcagaa gctgggtgga
    22741 cgaattccgc gcggtgtgct gatggtcggc cctccaggca caggtaaaac cttacttgcc
    22801 cgcgccattg cgggtgaagc aaaagtaccg ttctttacta tttcagggtc tgactttgtt
    22861 gagatgtttg tcggcgtggg cgcttcgcgc gtgcgtgaca tgtttgaaaa cgccaagaag
    22921 agttcacctt gcatcatttt tattgatgag attgatgccg ttggacgcag tcgtggatca
    22981 ggtactggcg gtggcaacga cgaacgcgag caaacactca accaattact ggtagagatg
    23041 gacggctttg aagcaaattc tggtgtgatt gtgattgctg caactaaccg cgctgatgtg
    23101 ttagacaaag ccttgcttcg tccgggccgc tttgaccgcc aagtgatggt aggcttgcct
    23161 gatattaaag gccgcgaaca aattttattg gtacacatgc gtaaagtgcc cattgatgtg
    23221 gacgttaaag ctgacattat tgcacgcggt acaccaggct tttctggtgc tgacttagca
    23281 aacttggtca atgaagctgc gctttttgct gcgcgccgca ataagcgcac tgtcgatatg
    23341 caagattttg aagatgcaaa agataaaatc ttcatgggcc ctgagcgtaa atcgatggtg
    23401 atgcgtgaag aagagcgccg caacacggct tatcatgagt caggccatgc ggtggtggcg
    23461 aaattgttac caaaagcaga ccctgtacat aaagtaacca tcatgccgcg cggctgggca
    23521 ttaggcttaa cctggcaatt gccggagttt gatcgtatca gtaactataa agataaaatg
    23581 ttggaagaaa tttcaatttt atttggcggc cgtattgctg aagaaatctt catgcatcaa
    23641 atgtcaacgg gcgcatccaa tgactttgaa cgtgccacca aacttgcccg cgccatggtc
    23701 accaagtacg gcatgagtga tgcgctgggt accatggtgt atgcgggtga tgagcaagat
    23761 tcatttttcg gtagcatgag cgctaaaacc atttctgaag caacccagca aaaagtggac
    23821 gcagaaattc gccgtatttt agatgagcag tacgcgcttg cacgtaagtt gctcgaagat
    23881 aatcgcgata aagtggaggc gatgaccgct gcgctgatgg agtatgaaac cattgatgca
    23941 gaccaaatca acgacattat ggccggcatt ccagtgcgtc caccaaagcc aacacaaagc
    24001 aaaaccagca cgccgcgtga ttctggcacc acaggtgtag gcgctgcacc ggtaccacag
    24061 ccatcagcta aatctgaata agcggtcaat ggtttaaaaa agggctagat ttctagccct
    24121 ttttttattg ccttgatttt aagtataatg ccacatggcc tctccttctg aatcaactag
    24181 cattttcatt tgcggcaagt accaactcaa acttaatcgc ccgcatgtga tgggcattgt
    24241 caatgtgacg ccggattcat tttctgatgg cggaaaatat acttctacgc aggctgcagt
    24301 ttgtcacgcg ttaacactga ttgccgaagg tgcggatatt ttggatattg gtggcgagtc
    24361 cacacgtcca ggggcagcgc ccgtgtcttt agatgaagaa ataagccgcg tgattccagt
    24421 gattgaggca ctcaaaggcg tttcaacggt gccgctttca atagacacct ataaaccaga
    24481 agtgatgcgc caagccattg cagcaggcgc tgatatggtg aatgatgtac gtgcattgca
    24541 ggagcctggc gcactggatg tgctagcaaa gagtcatgtt ggtatctgtt tgatgcacat
    24601 gcagggtaac cccatgacta tgcagatgtc accgcattat gaagatgttg tgattgaggt
    24661 gggacagttc ttggcagacc gtttgcaggt gtgtttaaac catggcattg ctaaaaatag
    24721 aattgtactt gacccgggtt ttggctttgg taaaacgcgc gaacataaca ttagcctcat
    24781 ccagcagctc gaaaagttgc atcgtatcgg cctgccattg ttagtgggtt tgtcacgaaa
    24841 atctgtgttg ggtgctattg cgggtggaga tgaaaatcag cgtttgtatg ccagtattgc
    24901 cgcatcggtc atttcggcca tgaaaggtgc aaagattgtg cgtgtccatg acgtgaaagc
    24961 aaccgtggat gcgcttaaag tggtggccgc gattctttag atgatgctca acacaaaatt
    25021 aggaaattat aaatgcgcac aaatgatagg gaaataccag cagaggcaaa ggatgcgatt
    25081 gctgagggaa agctaattca agctattaaa atcacgcgtg agaacacggg cttgagcctg
    25141 aaagaatcaa aagaacgcat cgaggcatat ttagaaactg acgcagtact caaagagaag
    25201 tatcttgctc atttgcaggc aaaagggctt tcgcaagaga gtgttttacg aatcgcaatc
    25261 attgggtttg tggcaatcat tgggtaccta atttttttta gatagggcct aaaaggtaaa
    25321 taaataagct aaaagttttt caccaaagat actaaaaatt aaacaagaaa gcgtaaaaca
    25381 tgagtaaaaa atatttcggc acagatggta ttcgcgggcg cgtgggtgaa taccctatta
    25441 ccccagagtt tgtgatgcgg ttgggctatg ccgcaggtaa agtgctcaca ggattgcagg
    25501 ttaacttagc caaaggtgcg caccctgcgg tgttaattgg taaagatacc cgcatttcag
    25561 gctacatgtt agaggccgcg cttgaggcag gcttgtctgc agcgggcgtg gatgtgttgc
    25621 tcaccggccc aatgcctacg ccagcggtgg cctatttaac ccgcgcgttg cgtgcgcagg
    25681 ccggtattgt gatttcggct tcgcataatc cgtatgatga caacgggatt aagttttttt
    25741 ctgccgatgg cgccaaatta cccgatgaca tcgaacacgc gattgaggcg gagctcgaca
    25801 aaccaatgca agtgatggaa tcagccaagt taggcaaggc acgccgtatt gacgacgcag
    25861 ctgggcgtta tgtggaattc tgtaagagca cgttcccaaa tcatctcgac ttacgcgggc
    25921 tcaaaattgt gctcgattgc gcgcatgggg ccacttacca cgtagcgcct gatgtgtttc
    25981 atgagcttgg tgctgaaatt atctgtatcg gcaatcagcc agatggcctc aacattaacg
    26041 aacaagtggg ttcaacgcat ccacaagcct tgcaaaaagc cgtggttgaa caccaagccg
    26101 atttaggcat cgcctttgat ggtgatggtg accgcgtgat gatggtggat gcagaaggtc
    26161 atttgttaga tggtgatcaa ctgctgtata ttatcgcgct gggtttatac gcaaaaggta
    26221 agctcaaagg cggtgtggcg ggtacattga tgaccaatct cgcgctggaa cacgcgctgc
    26281 aaaaacatca aatccccttc ggccgcgcca acgtgggcga ccgttacgtt ttagaattgc
    26341 tcaacactaa aaactggaaa ttgggcggcg aaaattctgg ccatatttta acgctagata
    26401 agcataccag tggtgatgcc attatcgcgg ccttgcaagt gctgcaagcg ctggtagaag
    26461 gtggcaaaac gctcgcacaa atgcgcgaaa aacttgtgct gtacccgcaa gtactgatta
    26521 acgtgactac taagcaaaag ctcgatttga atcatgcaag tattcaacag gcagtcaaac
    26581 aagcggaagt aacattgaat aacactggtc gcgtgttgct gcgtgcatcg ggcacggagc
    26641 caaaaatacg agtcatggta gaaggccaag accgcacatt ggtacaaaaa ctcgcagaag
    26701 acattgcaga agtggtaaaa aaagcggctg gttaagtagc aattggtcgc gacgttagcg
    26761 ctgtaaattt gcacattttt tcatatcaaa acagcacaaa attacactca accttttgag
    26821 atgccctgcg agccttataa ataaaggctc gcagggcatt gtttttctct cggggatttt
    26881 taagtggttt tactcggttt ttttagcaga aaaaacgcct taatttgtac ggcacgcaaa
    26941 accaatctat agaaatgatt taccaaaatg gtaagcggct gccgcaatta agccagaaca
    27001 tggaatcgtc agcacccatg cccaaacaat gcgtgaggcc aacccccaac gcaccgcaga
    27061 ggcgcggcgt gtgatgccta cgccaataat ggcacccgta atggtgtgcg tggttgaaac
    27121 tggtatgcca agataggtcg ccagaaataa ggctattgca cctgaagttt gggcgctgaa
    27181 ggcaccagaa gggcgaatgc gtgtaatgcc catgcccatg gtgcgtacaa tgcgccatcc
    27241 gccaaataag gttcctaaag ccatggcaat gtggcatgag ataatgaccc aaaattgcac
    27301 ttcaaagcct tgttgaaagc cattggcaac cagcagtacc gtgataatcc ccatggtttt
    27361 ttgcgcatcg ttgccgccgt gacccaagct gtagagcgaa gaagaaacaa actgcatttt
    27421 attaaatctt ctttcagtag ggtagggcga agatttttca aacagccaaa gtacgctcac
    27481 cattaaaatc atcgccagta acatgccaaa aagtggtgac aggataatag ccgcagacgt
    27541 tttaatgagg cccgctgcta caatcgctcc agtaccacct tttgcaaccc cagcaccaac
    27601 taaaccaccg accagtgcat gagaagatga tgaaggaata ccaagccacc acgtgatgag
    27661 attccagata atggcaccat tgagcgcgcc aaagatgacg gcattgttga caatatcggg
    27721 gtcaataatc cctttgccaa ccattttggc cacagcggtg ccaaaaataa caaacgcgat
    27781 aaagttaaag aaagctgccc ataatactgc tgcttgcggg gtaagcaggc gtgtggatac
    27841 cataagtgcg atggagttgg ctgcgtcgtg aaagccgttc ataaagtcga atatgagggc
    27901 taccaccacc agcaccgcca taatgacgat tgcgtgttcc atgttaaccc cttaaatgcg
    27961 ctctagcact acgccgtgaa tgacatcaga tacatcctga cagctgtcaa tcgcttcttc
    28021 aaataaatcg taaagcactt ttgaacgcat aatcgtacgt gcgtctgatt cttcagcaaa
    28081 caggcgttgt acgccagcgc gcatcacgtg atcaccttca ccttcaatca ggttgatttc
    28141 ttcacaggta cgtaaaatac gttcagcatt tttcatgtca cccagcatgt gtacggtatc
    28201 gcgcactttt tcagaggcgc gtaataaaat ttgtgcaagt gccaccattt caggggtaaa
    28261 gcttgagcgg ccgtatattt ttgcactttg tggaatatct tccataaagt cgataatgtc
    28321 atcaagcgcc attgccaact cgcgaatttc gcgacggtca aaaggcgtaa taaatgtttt
    28381 gtgtagtgag agtaaaattt ctttggtgta gccatctgcc tcagattcaa tttttcgtat
    28441 ttctgcgaag tgtacttcaa acttttcatc ttcactgacg gcagtcatca tcgctaatac
    28501 ttttgaggcg cgcaccgcgc aatcagccaa gttgttaaat aaaataaagt aattgtcgct
    28561 atgcggcgta atggcagaca tcaaccggct aaaaatacta cttttcattt ttttgctccc
    28621 taatttatta cagttttgtt tcatttttat aatatgcgta gtttaacctc ccgtgactta
    28681 attcgcgagg gcaaatgtca taaaaatagt aatttatctg cgatataatc catcaacacc
    28741 caatcaacca agaaattaaa ttcgcatgca acccaagtct atcgccacgc aaaagccaca
    28801 aattggcaca tttgaaaaaa atctcacttg gtgggtactg gcttgtattg tggtcggcat
    28861 attgttgggc cagatttttc ctgatttctt tcaagcggtg ggcagcttaa aagtggctga
    28921 agtgaatttg cctgttgccg ttttgatttg gctgatgatt attccaatgt tgttaaaagt
    28981 agattttggt gcaatgaaag aagtgttggc gcaccgccgt gggataagtg tgacgctatt
    29041 tattaactgg tttgtgaagc cgttttcaat ggcgttactg gcatggattt ttatccgaca
    29101 cctttttgta gcgtatttac cagcagagca aattgatagt tatattgccg gacttatttt
    29161 actcgctgcc gccccttgca cggccatggt gtttgtctgg agcaatctat gtaatggcga
    29221 tgccaaattt accctttcgc aagtggccat caacgatgcg attatgctcg ttgcctttgc
    29281 gccaattgtg gcgctcttac taggcttatc aagcattgtg gtgccgtgga atactttgct
    29341 gatttcggta atgttgttta ttgtggtgcc agtgatcatc agccaaatac tgcgcaaagt
    29401 tttgctcgca aaaagtcagt tggcgctgga aactacgctc aaacaattgc acccaatctc
    29461 gctgacagcc ttgctcgcca cgcttatctt attatttggc tttcaagggc agcagatttt
    29521 aaaccaaccg atgattattg ggctcttggc cgtgccgatt ttaatacaaa cctattttaa
    29581 ttcgatgtta gcctattggt tgaataaaaa atttaaagtg gcacattgcg tagccggtcc
    29641 atctgcatta attggcgctt caaatttttt tgagttggca gtagcaaccg ccattgcctt
    29701 atttggcttt aactcaggtg cggcgttggc gacggtggtt ggtgtgctga ttgaagtgcc
    29761 agtaatgttg tctgtggtgg caattgtgaa taaaagccgc gcttggtatg aagcttaaca
    29821 aatgcatgag gaagataaaa tgccagtaac gatttaccat aaccccgctt gtggcacatc
    29881 aagaaatacc ttggccatga tacgcgccag tggggtagaa cctaccgtaa ttgaatattt
    29941 aaaaacgccg cccactcgtg aaaaactgca agaaattatc agtaaaactg gcggcaacat
    30001 tcacgcactc attcgtgaaa aaggtacgcc atacgctgaa ttagggctag acaatcctca
    30061 tttatctgaa gaagcattgt tagacgcgat gttgcagcat ccgattctaa tcaaccgccc
    30121 gattgtagaa accagtaaag gcataaaatt atgccgccct tcagagctag tactcgatat
    30181 tttggataac gcaaacatag gcacgtttac caaagaagat ggcgaagtgg tgcatgcgcc
    30241 atccaaaagc gattagtgct ttactgtata atcgtgattt ttacaaacga tagtgcccat
    30301 catggaagcc gaacaactta acctcatcaa caaccgcttg gccgatttaa gcgagcgcca
    30361 tcttgccctt agggggtatc tttgactttg aaaacaagtc gcaaaagcta gaagaagtca
    30421 atggtctgct agaagaccca aaaatttggg ataacgctga aaaagctcaa aaactcggca
    30481 aagaaaaacg tagcctagaa ctggtggtgc ataatttgca gaacctagaa gcgggcatta
    30541 aagatgcgca agaattgttt gacatggcgc gtgaagaaaa tgacgatgat actctattaa
    30601 gcgttaaggc ggattctgaa gctttagaaa agcaaatcgc cgagatggag tttcagcgca
    30661 tgttttcagg cgaattggat aatgccaatt gctttgtaga gtttcaatct ggctctggcg
    30721 gcacagaggc gcaagactgg tgcaatatgt tattgcgcat gtatttacgc tacaccgagc
    30781 gcaaaggctt taaggtagaa gtgcttgagc tttcagacgg cgatgtggcg ggcattaagg
    30841 gtgcatcgct caaggtcacg ggcgattatg cttacggtac gttgcgcact gaaaacggcg
    30901 tgcatcgttt agtacgaaaa tcaccttttg attctaacgc taaacgccac accagttttg
    30961 ccagtgtgca gattttccct gaagtggatg attcgattca aattgaaatc aacccggccg
    31021 atttgcgcac tgatacttat cgcgcttcgg gcgcgggtgg ccagcacatt aacaaaacag
    31081 attctgctgt gcgtatcacg catattccca ccggcgtagt ggtgcagtgc caaaatgata
    31141 gaagccaaca ccgcaaccgc gacgaagcta tgaacatgct gaaaggcgcg ttgtataact
    31201 tagaacttaa taaacgcaat gcagagaaac aagccttaga agatgccaaa acagacattg
    31261 gttggggcca tcaaatcaga agttacgtgc tagaccaagg ccgcattaaa gatttgcgca
    31321 caggcgtgga aatcggcaat acgcaaggcg tgctagatgg cgaccttgac ccgtttatta
    31381 tggaaagttt aaagcagggt gtttagtttg tagctaaaaa cagcgtgtta aacgaaggac
    31441 atattttggt tttcttagct ggtgggcaca ccaatcatca ctttcgcctt ttcaacatcc
    31501 acgcgtaaaa actcagcaat ttcttggtag tcatcaggta gggccgcatc atcccagccg
    31561 ttggcagagt ggcgcgcgag gttaatggca aggcgcacgt tcaccgcgtg gtgttcggta
    31621 acattttcat catccatgat ttttgatagc aatggtggta gtttaaagtt ttcaaccagc
    31681 gctttttgta ggtctgaaat tttaaagccc agcacttgtt cctgagcggc tttgctgcgc
    31741 agggttttat cggcctgttg caagttggct atagttagca ttttattagg cgcaaagcac
    31801 cacagcagca tttcagctaa atcatgcagc agcgtggcga tatgcacttc atctgagcgt
    31861 aaatctttgt gataagccgc ccaatcggtg gcaaagtaag tggcgcggtg ggtgcgtttt
    31921 atgattttga gtaactgacc aagtgctggc aatttatctt ttagttggtc ttcaatcaac
    31981 aaagtggtgg gtaatttttc gtaaaacgca ttggtgccca tcatcaaaat ggcttgttcc
    32041 acctgaatta aatcttgctg ctggtgccga ctggggtgtt gttttgcgta acttaacacg
    32101 cgaaacacca tgaatggatc atgcagcaca atgggggtaa tgctccgcac ggtgaggttg
    32161 tcttcatctt ctttgagtag ttgcaactca cgtgcagtga gctttagcac ggggatgggc
    32221 ttgttgctca aaaaatgcac ccatgcggca acggcattgg tagcagtact ttggttggtg
    32281 gtcatacggc actctcctat attcacgcca tagcataaac cgtgacctgg ttttttaaag
    32341 tttggaatag aggccgaaaa tccccgtaaa taaaaatgaa tgaaagtgtt aaaataagcg
    32401 ttttagggca aaagtaagcc attaacttga gatgtgagcc attgtatgac cgatgaaacc
    32461 ccagtaattg atgaaaacca tgtcatttta gagcgtcgcg agaaattaaa agccatccgt
    32521 gaaaccgcaa aggctaatca gggcgcagcg tttccaaacg attttaaacc acaacataag
    32581 gcggcagatt tgcatgcgca gtatggtgct tttgagaaag aagcgctaga accgctggcg
    32641 gtgcaagtgg tggtggctgg gcgcatgatg ttgaagcgcg tgatgggtaa agcgagtttt
    32701 gcgaccattc aagacggcag tggacaaact gctggcggcc gtattcaact gtacattgcg
    32761 cgcgatgaaa ttggtgaagc cacatatgaa agttttaagc attgggattt aggcgacatt
    32821 ttaggcgcaa cgggtaaagt gtttaaaacc aaaacgggtg aactctctat tcatgtcagt
    32881 gaaatcaaat tgctgactaa atcactgcgc ccattaccag aaaaatttca cggcttggct
    32941 gaccaagaag tgaaatatcg tcagcgttat gttgatttaa tgacttcaga agaaacccgt
    33001 gctactttta ttgcgcgtag caaagtggtg tcggcaattc gcaactttat gattcaaaat
    33061 gaatttttag aagttgaaac gccaatgttg cacccaattc ctggtggcgc atcagcgaaa
    33121 ccgtttatta cacaccacaa tgcgctcgat atgcaaatgt tcatgcgcat cgcgccggag
    33181 ttgtatttaa aacgcttggt ggtgggcggt tttgagcgcg tgtttgaaat caatcgtaac
    33241 ttcaggaatg aaggcttaag cgttcgccat aatcctgaat ttaccatgat ggagttctac
    33301 gcagcttata cggattatca atggttgatg gattttaccg agcaatgtat tcgcgctgcc
    33361 gccattgccg cgcgcggtac agcggtgttg agttacgatg gcaaagaggt ggatttgagc
    33421 aagccgtttg agcgccttac cattgtggag gcaattcaaa aatatgcgcc agaatatagc
    33481 cttgcgcaat taaatgatgc acaattttta cgcgctgacc tctacaaacg cggcattaaa
    33541 gtgtttgaac acgctggttt gggctcgctg caattaagcc tgtttgaaga aacggccgaa
    33601 agccaattgt ggcaacccac gtatattatt gattaccccg ttgaagtgtc gcctttggcg
    33661 cgcgcttcag acaaaaaccc ggaaatcacc gaacgttttg agttattcat taccggccgc
    33721 gaaatggcca atggttttag tgagctcaat gatgcggaag atcaagctgc gcgctttcac
    33781 gcacaaatga aagccaaaga agcgggcgac caagaggcga tgtattacga tgcagatttt
    33841 attcgcgcgt tggaatacgg catgccacca gcgggcggct gcggcattgg cattgaccgt
    33901 ttggtcatgt tgattacgga ttcgcccagc attcgtgatg tgattttatt tccgcacatg
    33961 cgcgcagaga attagtgttt ttagaggcgc aattcatcgt aattttaaac acataaaaac
    34021 tacgagaaaa aataaatgct ctggaagcct ttgtttataa ggctcacagg ccatcaaaaa
    34081 aggttgtcgt taattttagg ctgttttaac tgatttttta gtctaaattg cccccttaaa
    34141 aaagccatgc aacactgcat ggctttttta atttttgtca caaagttgtc atattcgcac
    34201 tctagaatgc aatgcatcga aacagcatgt atttaacttt atggagaaaa gcgacatgaa
    34261 taaaaaacta ggcatggcag tgattattgc agcagcattt accagcggct acgcaagcgc
    34321 agcggataaa gtgattaaaa ttgatggttc aagtacggtt tatccagtca ctgaggccgt
    34381 tgctgaagag tttcaaattg cgacaaagaa caaagtgacg gtgggtgaat ctggtactgg
    34441 tgctggcttt aaaaaattct gccgtggaga aaccgatatt tcaaacgctt cgcgcccaat
    34501 tgcacaaaag gaaatggacg cttgtaaaga agcgggcatt caatttattg agttacccat
    34561 agcctttgac gcgctcaccg tggtggtgaa tagcaaaaac gattgggtta aaagcttaag
    34621 cgttgaagag ttgaaaaaaa tgtgggagcc aaaatcaagc gttaaaaact ggaagcaagt
    34681 gaatgcggct tatccagaca aagcgctggc tttatttggc ccaggtaccg cttcaggtac
    34741 gtttgattac tttacagaag c
//
LOCUS       DAOQSI010000005        34137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_686_length_34137_cov_5.389384, whole genome shotgun sequence.
ACCESSION   DAOQSI010000005 DAOQSI010000000
VERSION     DAOQSI010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 34137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34137
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_686_length_34137_cov_5.389384"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="PKL42_00775"
     CDS             complement(<1..972)
                     /locus_tag="PKL42_00775"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNU65629.1"
                     /translation="MQISTQKKSLGFKLAFKTQIATALLLSTFVPYTQAAEAQIAPAP
                     NLAVKAYLLKDLNSGFIIAEQQGNMRVEPASLTKIMTAYLSFKALKNGHLKLTQTLPV
                     SEAALKVEGSRMFIEPRIPVTVDELLHGMITQSGNDASIALAEGIASTEAQFADMMNK
                     EAQRLGMKNTHFVNATGLPDPQHYTTAADLALLAGALIHDFPEDYQRLYSVKEYTYNK
                     IKQQNRNRLLWLDPNVDGMKTGHTESAGYCLIASAKRDGVRRVSVVLGAANDAARASE
                     SQKLLNYGFQFYDTSTVYKQGQTISQLKVWKGAEEQVASTVAQDYLIT"
     gene            complement(987..2096)
                     /locus_tag="PKL42_00780"
     CDS             complement(987..2096)
                     /locus_tag="PKL42_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNU65630.1"
                     /translation="MHLTKANLQKCTILFSIILLSACGGVTTKPSSKEPSAPTSQSSD
                     TSTAKPGAGGYYLDDGPGEGAPADIDSIPNAQITNEPPLERSNKPYSALGKNYVPLTS
                     YKPYKKQGVASWYGKRYHGKKTSSGEVYDMYAMTAAHTTLPLPSYAKVTNPANGSSVI
                     VRINDRGPFKNDRLIDLSYAAAYKLRLIEQGSGLVEVELINASNNEAVIAATSKTAPV
                     NEVKPAATPVSKPAEPSPIMPEPEVVATPVEVAPPVKPVPEVITPSEPKTVAAAPVAD
                     ITPPAQAPATQENSASKPGYYVQAGAFRSEANGQQLQKKIQRLGVAGNASVTNVYNDG
                     LYQVKLGPYATKKEADLAASNLRKQLSITPIVKKQ"
     gene            complement(2129..3253)
                     /gene="rodA"
                     /locus_tag="PKL42_00785"
     CDS             complement(2129..3253)
                     /gene="rodA"
                     /locus_tag="PKL42_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNU65631.1"
                     /translation="MISRLFEIIGKHIDAFLMTCLLVTLLLGLSVVYSASVPYMYKQA
                     VNITVALGCMWIVANLTPHQLQRLAVPLYILGVILLIAVALFGSSSKGAQRWLNLGFI
                     KIQPSELMRIAMPMMLAWYFAKCEGRPYGRDFAAATLLLVIPVGLIKIQPDLGTSLLI
                     FASGFYILFLAGLNWKLLAGSAATLAVATPLLYIMRQPILDFFIHHGLLHGYQRKRIE
                     ILLGDIQDPLGAGYNTIQSVIAVGSGGFNGKGWLNGTQTQLDFLPEHHTDFIFAVFSE
                     EFGLMGNLLLLSLFSLIILRGLYIASEAKNTFSRLLAGSLALTFFTYAFVNIGMVSGI
                     LPVVGVPLPLISYGGTSMVTLCVSFGILMSIQTHKNLVGT"
     gene            complement(3246..5201)
                     /gene="mrdA"
                     /locus_tag="PKL42_00790"
     CDS             complement(3246..5201)
                     /gene="mrdA"
                     /locus_tag="PKL42_00790"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNU65632.1"
                     /translation="MLSELKNFQHEQYYFKRRLVFAGFVVVALFLLLAYRLFYLQVIQ
                     HQHYQTLAENNRISVVPIVPTRGLILDKNGVVMAHNFFVYTLEIIPSKLDTNIEDTIN
                     ELSHLIEISELDKKKFKKLRQASRNFESIPIRTHLNETEAASFAVNKYQFPGVEIKSR
                     LYRRYPLGKLGAHIVGYIGRINDKDVANLEEADKENPGVLSNYKGSDHIGKSGIEQFY
                     EEQLHGKTGFQQIEIDKDGRAVRVLSSTAPVPGENIVLSIDTKLQEIAEKAFGDRRGA
                     LVALNPKTGEVLSFVSQPNFDPNLFVDGIDVDNWKALNDSIDRPLINRPIRGVYPPGS
                     TFKPFVALAGLEHGLRAPPYAIHDAGYFSLPNSTHRYRDWKPSGHGIVNMQKAITISC
                     DTFFYGLAMELGIGNLSDFVSHFGFGKKTGIDIQGENGGLLPTPDWKKRRYKQPWYQG
                     DTVITGIGQGYTLATPMQLAYGTAILANSGVAMKPHLVTKMQNPLTGKTVIIPEVIQD
                     KITIHPDNLEIVKKGMIDVTLPGGTAASVGANAPYSVAAKTGTAQVIGIKQNAKYNAN
                     AINERHRDHALFVAYAPADDPTIALAVIVENGGHGGSAAGPIARKVMDYYLLNKLPEV
                     DSVKDTANVPAENAVDAESIMEEEFHD"
     gene            complement(5291..5770)
                     /gene="mreD"
                     /locus_tag="PKL42_00795"
     CDS             complement(5291..5770)
                     /gene="mreD"
                     /locus_tag="PKL42_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNU65633.1"
                     /translation="MPHNNFKTVYISLLVALICLLVPWSGLALKARPDFLLLVVIFWL
                     IRAPNLCNIGTAWLVGLIADLASGGIFGQYALAYTVTAFFGVVYQRRLVLFNHTQQLL
                     YVFILLLISQFTLLILKVFSGAEVWGWSYFLPSFTGILLWRLAVLFGLNTGGRARGT"
     gene            complement(5757..6755)
                     /gene="mreC"
                     /locus_tag="PKL42_00800"
     CDS             complement(5757..6755)
                     /gene="mreC"
                     /locus_tag="PKL42_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNU65634.1"
                     /translation="MALGFNHKLEQQQEPAFFVRGPSPFARLVFFSALSLALIATDSR
                     LQYLSTVRQHLVAILHPLQVIANAPSQLYQNVTEYFSTQNRLVSENKQLRQQALENNV
                     ALQKLNTLTLENSHLRGLLKTNQALAETSVLGEVIYVGRDLFRKKNILNRGTQHQIIA
                     GQAVVDATGVIGQVTRVFPYSSEVTLITDKALAIPVQIERNGLRAIAFGRGRDNTLDI
                     PYLPANVDIKTGDKLVTSGIDGVYPAGLAVATVTKITATPDSAYARITCTPTGGIENH
                     RHVLIVSMPIKNEVPSQEVETSQNTLPTSNKKESGQTKIKKAANLTEKPIKPNATQ"
     gene            complement(6957..8000)
                     /locus_tag="PKL42_00805"
     CDS             complement(6957..8000)
                     /locus_tag="PKL42_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNU65635.1"
                     /translation="MFGFLTSYFSNDIAIDLGTANTLIYSRGKGIVLDEPSVVAIRQE
                     GGPGGKKILLAVGAEAKNMLGRAPASIQAIRPMKDGVIADFTITEQMLKYFIRRVHDA
                     RWFSPSPRIIICVPVGSTQVERRAIKESAERAGAKQVFLIEEPMAAAIGAGMPVSEAT
                     GSMVVDIGGGTTEVGVISLGGIVYAKSERVGGDKFDQSIIDYIRRNYGMQISEPTAEA
                     IKKKIGSAFPGSEVLEMEVTGRNLAEGLPRKFTISSNEILEALTEPLNTIVGAVKSAL
                     EQTPPELGADIAEKGMVLTGGGALLRDLDRLLVEETGLPVIVAEDPLTCVVRGCGRAL
                     DEMDKLGSIFAYE"
     gene            8252..8566
                     /gene="gatC"
                     /locus_tag="PKL42_00810"
     CDS             8252..8566
                     /gene="gatC"
                     /locus_tag="PKL42_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNU65636.1"
                     /translation="MALSIEDIKKVAHLARLEISDADASATLTKLSGILRLIEQMQAV
                     DTTGIVPMSHSQDLSQRLRDDIVTKTNQREAFQKNAPVLGNGSTESAVAEGLYLVPKV
                     IE"
     gene            8568..10022
                     /gene="gatA"
                     /locus_tag="PKL42_00815"
     CDS             8568..10022
                     /gene="gatA"
                     /locus_tag="PKL42_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNU65637.1"
                     /translation="MMNRSLKQFGEMLQTKKISSVELTQTFLARIAQHNPSINAYIAL
                     DEAKSLAQAKAADALIAAGKASPLTGIPIAQKDIFCAKDWPTTCGSKMLANFVAPYDA
                     HVITQFDAVGAVNLGKTNMDEFAMGSSNETSYFGGVKNPWDVERVPGGSSGGSAAAVA
                     ARLCAAATGTDTGGSIRQPASLCGFSGLKPTYGVVSRYGMIAFASSLDQAGPMAKSAE
                     DCALMMNVMAGFDERDSTSLNREKEDYTRDLAKPLQGLRVGLPKEFFAEGLDASVGKV
                     IDEAIAAYKKLGAEIVEISLPNTGLSIPVYYVLAPAEASSNLSRYDGVRYGHRTAEYT
                     DLMDMYCKSRAEGFGAEVKRRILIGTYVLSAGYYDAYYLKAQQIRRLIAQDFVEAFKH
                     CDVIMGPAAPSTAFKAGEKVDDPVAMYLQDIYTISTNLAGLPGMSVPAGFSNGLPVGL
                     QIIGNYFDEARMLNVAHQYQQVTDWHLKMPQGIE"
     gene            10022..11497
                     /gene="gatB"
                     /locus_tag="PKL42_00820"
     CDS             10022..11497
                     /gene="gatB"
                     /locus_tag="PKL42_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNU65638.1"
                     /translation="MEWEVVIGLETHTQLKTNTKIFSGASTAFGAEPNTQACEVSIAL
                     PGVLPVLNREAVNCAIKFGLAVNAEIAPRSVFARKNYFYPDLPKGYQISQYELPVVGK
                     GAVTIQVPAVGKNPAYEKVVNITRAHLEEDAGKSVHGAVEGMSGIDLNRAGTPLLEIV
                     TEPDMRSAAEAVAYAKKLHELVQWIGICDGNMQEGSFRCDVNVSVRPKGTEKLGTRRE
                     IKNLNSFEFMQQAIEYETRWQIETLEDGGTIQQATVLFNPDTGETRSMRSKEEANDYR
                     YFPDPDLLPLEVTEADKARVKAEMKESQEAIKTRFETQYGLNAYDVNVLTASRAKADY
                     FERVVIGCGDAKLAANWVIGELSALVNRLDFYSENPLADNPPVDANDLAQLLNRYKGG
                     NLNRAQAKEVLVDIANQSFSNLGETVDQIIARRGFVQISDSGAIEAIIDEVLKANPAM
                     VEEFKAGKEKAFNALVGQAMKASKGKANPAQVNEILKKKLS"
     gene            complement(11690..12547)
                     /locus_tag="PKL42_00825"
     CDS             complement(11690..12547)
                     /locus_tag="PKL42_00825"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNU65639.1"
                     /translation="MDDLKRRQNLFACASRSLLWIACLFLIFTISPQASAEGKIVKWK
                     DANGVTHYGDKLPAEAAGRSNSEINKTGVTVKKNEAYNEKTDSGEVERTNTSQSRRDS
                     ALLASYGSIDEIDLARDRNIKSDELALANLNQRLNDTRVTLQKNKEKQEGFLKRKKPV
                     PNNILDDIKYNQAQITKTQAEITAVEKNIAVTMARFASYKARYAELKPNNDKSPVVIE
                     GKKRLADLEAQKAATQTRLNGLLKEALDAKKAGVEQPQRVSFGIQEANEEIARLNLEI
                     MATQAKMKE"
     gene            complement(12659..13339)
                     /gene="pyrE"
                     /locus_tag="PKL42_00830"
     CDS             complement(12659..13339)
                     /gene="pyrE"
                     /locus_tag="PKL42_00830"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNU65640.1"
                     /translation="MSQQDKTLAAEKQFSQEFIAFAVKKEVLRFGEFKTKAGRNSPYF
                     FNAGLFNDGESLMKLGEFYAAAIQNSGIAFDMLFGPAYKGITLVASIAIAFAKNGHNI
                     PYAYNRKEAKDHGEGGVIVGSPLKGRVLIIDDVISAGTSVRESVELIKVNGATACGVA
                     IAIDRQEKGLGELSAVQEVVQQHQLPVCAIATLSDLLNYLQDKPELAQYLHAVQRYRQ
                     QYGVIPLN"
     gene            13379..14170
                     /locus_tag="PKL42_00835"
     CDS             13379..14170
                     /locus_tag="PKL42_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNU65641.1"
                     /translation="MKIISLNLNGIRSASNKGLYAWLKKQDADIICLQEIKAQAADMT
                     PEMLNPKGYFGYFHYAEKKGYSGVGIYCKQKPDRVIEGLSHAHDDVLDIDAEGRYIEA
                     RFDNLSVVSLYLPSGSSGEERQAFKFSVMNRFLPHLKALKLSGRDVVICGDWNIAHQE
                     IDLKNFKGNRKNSGFLPEERAWLSTLFDEVGWVDVYRKLHPTTTDESYTWWSNRGQAW
                     AKNVGWRIDYQIATPAFAAKAVSTSIYKAERFSDHAPLIVEYEDA"
     gene            complement(14174..15532)
                     /locus_tag="PKL42_00840"
     CDS             complement(14174..15532)
                     /locus_tag="PKL42_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HNU65642.1"
                     /translation="MLICIFTGFSSGLPLFILISLLPAWLRSEGVSLKAIGLMALILF
                     PYTWKFLWAPILDRYTLPLGRRRGWILLTQLVLLASVFWFGSVSAKNLTAPVFESHAY
                     FNGLFSLSLPVWSTVKTFLSHPITIFSFIVAIFSATQDIALDAYRRELLLDHELGLGN
                     AVHVNAYKIAGLVPGSLSLILADHLTWNHVFMITALFMLPGIIMTLMVSEPMLKKGAP
                     RTLRAAVVEPFKEFIGRNGMQTALLILAFIFLYKLGDSMATALATPFYLDMGFSKTEI
                     GLIAKNAGLWPSVIGGLLGGVWMIKLGINRSLWLFGIVQMVAILGFAWLARAATDPAL
                     FNAFFEFNFLGYQFNLQERLVWLAIVIGIEAFGVGLGTAAFVAFIAHTTHPLYTATQF
                     ALFTSLAAVPRTFANAATGYMVESLGWFKFFILCFLLAIPGMLLLLHIAPWNKTAESI
                     KL"
     gene            complement(15586..16206)
                     /gene="metW"
                     /locus_tag="PKL42_00845"
     CDS             complement(15586..16206)
                     /gene="metW"
                     /locus_tag="PKL42_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HNU65643.1"
                     /translation="MQNNVNNTNYVSEFRQDFAIIANWVKFGTKVLDLGCGDGELLQY
                     LRNSLEVKGYGVEKDDAKWLACMQNGSNVIQMDLEDGLSGFEDQSFDTVILSQTLQAM
                     HHTEEIVQEMLRVGREVIVTFPNFGYWRNRLQILTGNMPVSKTLPYQWFDTPNVHLCT
                     ISDFDQFCKNHNITVLERRVITDTQAVEFMPNLLGNLAMYRLKASS"
     gene            complement(16196..17338)
                     /locus_tag="PKL42_00850"
     CDS             complement(16196..17338)
                     /locus_tag="PKL42_00850"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HNU65644.1"
                     /translation="MLENNSVGIVAPQNAHFDTPLTLKSGAVLPTFDLAYETYGTLNH
                     DKSNAVLICHALSGTHHVAGKYSENDKYPGWWDNLVGPNKPLDTNKFFVIGVNNLGGC
                     HGSTGSASINPATGKPWGSAFPIVTVEDWVNSQARLLDFLGLPSLAAVIGGSLGGMQA
                     LQWTLAFPNRVRHALVIASAPNLTAQNMAFNEVARQAIITDPEFHGGDYYAHNAIPRR
                     GLRIARMLGHITYLSDDAMGAKFGRKLKNDIQYSFDAEFEMESYLRYQGDKFAGEFDA
                     NTYLRMTRALDYYDPALPFNNDLTAALKQAKAHFLVVSFTSDWRFSPARSREIVKALL
                     DNELTVSYAEVTAAHGHDAFLMPDAHYHNILRTYLSNIEIGGNDAK"
     gene            complement(17580..18449)
                     /gene="prmC"
                     /locus_tag="PKL42_00855"
     CDS             complement(17580..18449)
                     /gene="prmC"
                     /locus_tag="PKL42_00855"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNU65645.1"
                     /translation="MPNTQSIKSALAFATVKLNHDEAKLEAQLLLQHVLNVNRAWLIA
                     HENDALQANQHAAFEALLNRRLNGEPIAYILGGRAFYGLDLLVTPDTLIPRPDTETLV
                     EAALAQIPIAAHPSILDLGTGSGAIALAIAKNRPQASVIAVDASKAALAVAKKNAQNL
                     SIDNVEFVLSNWFQHLNNQPFDVIVSNPPYIEENDAHLSQGDLRFEPISALSSGEDGL
                     NDIRQIIENSLVHLQPQGWLMLEHGFNQADKVADLMANTGFVSIETIKDFGGNNRVTI
                     GKNPLIVSTHWAE"
     gene            complement(18529..19611)
                     /gene="prfA"
                     /locus_tag="PKL42_00860"
     CDS             complement(18529..19611)
                     /gene="prfA"
                     /locus_tag="PKL42_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNU65646.1"
                     /translation="MKPSMMTKLANLSERLEEINRLMSSEGVTNNMDNYRKLTQEHAE
                     ITPIVEQYHAFTQAEADIKEAQAMLSDPDMKEFAQEEIAAGKAKLEAVESALQILLLP
                     KDPNDEKNIILEIRAGTGGDESGLFAGDLFRMYSRYAEKQKWKVEVLSANEGEVGGYK
                     EIIAKIEGYGAYSKMKFESGGHRVQRVPDTETQGRIHTSACTVAILPEVDEVSDVVIN
                     PADIRLDTFRASGAGGQHINKTDSAVRITHFPTGIVVECQDGRSQHSNKAQAMMVLAA
                     RIKAKQVDEQNSKIASERRNLIGSGDRSERIRTYNYPQGRITDHRINLTLYKIDAITE
                     GDMDELIGALSAEHQADLLASLGDDN"
     gene            complement(19639..20883)
                     /gene="hemA"
                     /locus_tag="PKL42_00865"
     CDS             complement(19639..20883)
                     /gene="hemA"
                     /locus_tag="PKL42_00865"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNU65647.1"
                     /translation="MQLYVIGVNHTTAPIQIREHVAFNSDRLAGALRELTLHDATEAS
                     ILSTCNRTELYCSTDNPQKPLNWLSQYHKLDKDAIQPYIYTLPNDEAVKHAFRVASGL
                     DSMVLGEPQILGQFKQSVKIAQEAGTLGTLLHKLFQRTFEVAKEVRTNTDIGANSVSM
                     SAAAVKLAQRIFGNISEQKVLFIGAGEMIELCADHFAAQKPKQMTIANRTLERGAALA
                     EKIGGEAILLQELPAHFADYDIIISCTASQLPIVGLGMVERAVKTRRHRPIFMVDLGV
                     PRDVEPEVKDLDDVFLYTVDDLAQVVQEGLGNRQQAAADAEIIVASRVEHFMHWLKKR
                     DGIPTIKALRDQAENTRKAELDKALKLIQKGESPEKALELLSNALTNKFLHAPSHALN
                     QTHGEEHTRLEQTLRQIYQINH"
     gene            complement(20925..21284)
                     /locus_tag="PKL42_00870"
     CDS             complement(20925..21284)
                     /locus_tag="PKL42_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HNU65648.1"
                     /translation="MYFHLKPILFAITLAALLSACSSINLNPFSTGSKENARTPQDAI
                     TYLCEGNKQFHVRMLNNGNEAWLIYPDHEVSLPKSSSGAGRYSSGAISLVINGDETTL
                     NDGEKIAYTACKAQTKK"
     gene            complement(21320..22330)
                     /locus_tag="PKL42_00875"
     CDS             complement(21320..22330)
                     /locus_tag="PKL42_00875"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNU65649.1"
                     /translation="MHVSLAQASTILKSGEVVAIPTETVYGLAADASNDSALQKIFAT
                     KQRPADHPLIVHIGNINQVYSWVTDFPETAVKLAQTFWPGPLTLVLPAQDHVSRVLRG
                     NEATVALRVPNHPTTLALLELSGLSVAAPSANLFTQLSPTTAEHVEMGLGTHVPVLDG
                     GACAVGIESTIVSVASNGNWQLLRPGMISEAEIALVAGRPPMNVDKTNRNNLDTLTPK
                     VPGQHLLHYSPRTSVYLFNNRADLVHACEAIQLANQTCAALLIGDGDKPSCKIMRLIN
                     QPQEVAEALYRTLHELDALQVNQLLVELPPDTPIWAAVRDRLTRAGGSATESLRSQVI
                     SK"
     gene            22474..22806
                     /locus_tag="PKL42_00880"
     CDS             22474..22806
                     /locus_tag="PKL42_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNU65650.1"
                     /translation="MAVNQITVENNPAQSRLDALGVSKWPTWQKEVSTFSWTFPEQEI
                     AYILDGECVVTTCCGNVVSFGKGDLVTFPAGTKITWEVKQPLHKNYQLDGTLIGRIYR
                     RIKIALKL"
     gene            22819..23385
                     /locus_tag="PKL42_00885"
     CDS             22819..23385
                     /locus_tag="PKL42_00885"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNU65651.1"
                     /translation="MLLMIDNYDSFTYNLVQYFGELGQDVQVYRNDEIDLKKVAALKP
                     RHIVISPGPCTPNEAGISVPLIHEFAGKIPLLGVCLGHQSIGQAFGGKVIHAKQLMHG
                     KTSLIHHKNMGVFKGLPNPYTATRYHSLVIERETIPDCLEITAWTDDGEIMGVKHKTL
                     AVEGVQFHPESILTEFGHELLDNFLKAY"
     gene            23400..24413
                     /gene="trpD"
                     /locus_tag="PKL42_00890"
     CDS             23400..24413
                     /gene="trpD"
                     /locus_tag="PKL42_00890"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNU65652.1"
                     /translation="MFKQALNQLLAREDLSFDTMLSVMQQVMGGELTPAQIAALLVAL
                     RMKGETVEEISAAATVMRSLSTKVDIQDHAYLVDTCGTGGDGIQTFNVSTACAFVAAA
                     AGAKVAKHGGRSVSSTCGSADVLEVLGVDVNRTPAQVANSVNAIGIGFMFAPNHHSAM
                     KHAAPVRRELGVRTIFNLLGPLTNPASAKRQVMGVFDKHLTTKLAHVLQHLGAEHVLV
                     VHGEDGMDEISFSGDTFVAELKNGKIHEYTLNPAHFGLPHHDLSQIQVENAEQSKAII
                     LDVLNGKRGAARDIVLLNAGAAIYVSGAVDSLADGVKKAAEVIDNDAALAKLEALKKQ
                     VAA"
     gene            24410..24874
                     /locus_tag="PKL42_00895"
     CDS             24410..24874
                     /locus_tag="PKL42_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3465 domain-containing protein"
                     /protein_id="HNU65653.1"
                     /translation="MKKYLFLFILLSALVACKQNTSSLSAGNTVPAVQSEDKGLAVSP
                     NENASLIEAFQHKKSNLFVEGRGVIKKLLPDDNKGSRHQKFLVTISDAQTLLFAHNID
                     LAKAVPIAVGDTVQFRGEYVYNPKGGVVHWTHHDPKAKIEGGWIKHNGQIYQ"
     gene            24887..25678
                     /gene="trpC"
                     /locus_tag="PKL42_00900"
     CDS             24887..25678
                     /gene="trpC"
                     /locus_tag="PKL42_00900"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HNU65654.1"
                     /translation="MSDILNKILATKQQEIAKSKAVVSLAQLQKQAEAQGDPRDFVGN
                     IFAKVSANKPAVIAEIKKASPSKGVIREDFNPAEIAKSYEKGGAACLSVLTDVEYFQG
                     SAEYLKQARKACKLPVLRKDFMIDPYQVFEARAMGADCILLIVAALELNQMRALEQLA
                     HDLGMAVLVEVHDADELNMALQLDTPLIGINNRNLRTFEVTLQTTLDLLKVLPEDRFV
                     VTESGIFTPEDVKLMQDHDVHAFLVGEAFMRQPDPGAELARVFGL"
     gene            25712..26707
                     /gene="hemB"
                     /locus_tag="PKL42_00905"
     CDS             25712..26707
                     /gene="hemB"
                     /locus_tag="PKL42_00905"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNU65655.1"
                     /translation="MSYPYSRPRRLRKDEFSRRLVRENVLTTNDLIYPVFVLDGENRV
                     EEVASMPGVKRQSIDILLKTAAECVQLGIPAIALFPVVDSQFKSLDAAEAYNPEGLAQ
                     RAVAALKAAYPTLGVITDVALDPFTTHGQDGLIDDSGYVLNDETIEVLVKQAISHANA
                     GADIVAPSDMMDGRVGQIREALESSGNIHTKILAYSAKYASAFYGPFRDAVGSAGNLG
                     SGNKYTYQMDPANSDEAMQEVALDISEGADMVMVKPGLPYLDIVRRVKDEFGVPTFAY
                     QVSGEYAMLKAAAQNGWLDEKACVMESLLAFKRAGADGILTYFAMDVARWLKVKD"
     gene            26716..27138
                     /locus_tag="PKL42_00910"
     CDS             26716..27138
                     /locus_tag="PKL42_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65656.1"
                     /translation="MNNEQIRFMYEKYMDIVKLELEVEEFGCRPTEVRHLIGRLGEFF
                     CAIHVNGKLAHEANQHGFDVIAENGRRISVKTTAQKSGFITINPNTINKVDDLMLIQF
                     IDSRLEVVFHGPIQKAVDVSRTWEGKFEFDISKAKKLT"
     gene            complement(27481..28107)
                     /locus_tag="PKL42_00915"
     CDS             complement(27481..28107)
                     /locus_tag="PKL42_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65657.1"
                     /translation="MAKENPTQLRQLIAQKAAQMMAEDGISDFSYAKNKAGRQIGALD
                     NSVLPSNTEIEEELKRYNALFLSEAQPENLRALRKNALFTMQLLEKFNPYLTGAVLDG
                     TAGLGTETHIHLFADSLKEVEMFLLNQDIPFETNEKSYRILNDGKRDKKMDARKKVPI
                     FSLETITGIIKLSVFEFDEIRVPTKRAANGSNAERADISTVKNLIANT"
     gene            complement(28132..30642)
                     /locus_tag="PKL42_00920"
     CDS             complement(28132..30642)
                     /locus_tag="PKL42_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HNU65658.1"
                     /translation="MTLNKWWHYIFLAIFVSCMSVAALIAIAAAIIYPSLPSLEALTD
                     YHPKLPLQVFSEDGHLIGEFGEERRAYIKIENVPQAMKDAVLAIEDRRFYQHAGVDTT
                     GVVRAIKNNITGKGREGASTITMQVAKNFFTPPNGKRTIITKINEALLAVKIERNLSK
                     DKILELYLNQIYLGQRAYGFAAAAQAYYDKPLEKLNLAEIALLAGLPKAPSGYNPFTN
                     PKRAIGRQHEVLRDMLRFGFLKEEAYQEALKQPLSFKSAKQERSSLSADYVAEIVREN
                     LYAKYQDEIYTSGLKVYTTIRKANQLAANNAVREGVLDYDMRHGYRGPEKILTLDAIT
                     SRDDKTALDDALDEFEVYANLIPAIITKITPKSIFAHTKYGDDIEVTGNGLKLIEKTL
                     KEKDPEKRLLKPGAVIRVIKTATKKSDGTNLAEAWRVVQLPEVESAFIAIDPETGAVR
                     ALVGGFDFSRNKFNHVTQAHRQPGSSLKPFIYSAALEKGYTAASIVDDAPLSFSAAET
                     GDDAWSPQNYDNDFRGPVRLRKALTQSINTVAIRILEKIGPSYAQSYLTRFGFKAKDH
                     PPYLTLALGAGSTTPWEMATAYAVFANGGYLVKPNIITKIVDSNGKVIMQSKYQRAGK
                     DAPRVIDARNAFIMNSMLQSVVQNGTATKALSLKRTDIAGKTGTTNDHKDAWFTGYSP
                     KQVGIAWIGFDKPRNMGRGETGGAAALPIWIKYMAVALNGVPEMAMRMPEGVVAINID
                     PATGTRSSSGILEYFYHENPPPTHEEPLPPLFGEEELTDDNAETDGEDANAQQNQAQK
                     VLQPEVVLSPPNNAANQAKPENSNADPQNAAAKMLNTN"
     gene            30889..31962
                     /locus_tag="PKL42_00925"
     CDS             30889..31962
                     /locus_tag="PKL42_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HNU65659.1"
                     /translation="MKFNLFTESTPPLVGVDISSTAVKMVELSDNGRGGYKLEGYSIS
                     ALPKEAIVDGNIGDLEKVVDGVKLAWKLLGSREKRVALALPASAVISKKVLMSAGLRE
                     EDMEVQVEAEANQYIPFSLDEVNIDFQVIGAAPNNPDEVEVLIAAARKEKIEDRVAAA
                     EGAGLKVTVMDVENYATEAAYSLVASQLPNSGREQTVMIVDIGASMMHVNVLHDNKSV
                     YMRDQSFGGGQLTQEIQRRFGLSTEESEIAKRKGGLPESYEGEVLQPFMQALATEVAR
                     AVQFFTSSTQYNRVDNILLAGGCAAIPSVDVLVQERTQVHTVVANPFHSMTISSKLKQ
                     QQAAIDAPALFVACGLAMRGLSI"
     gene            31959..32564
                     /locus_tag="PKL42_00930"
     CDS             31959..32564
                     /locus_tag="PKL42_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HNU65660.1"
                     /translation="MIRVNLLPHRQVKRAERQREFGLMAAFAAFAAAAIIFLGYTFIS
                     SQVEAQTARNSRLDEAIVKLDKEIADIKDLKDQISIMLDRKQVVENLQTNRSQSVVVL
                     DEISRQLPEGMYLKSIKQQGNSITLEGVADTNSRVATLVRNFSTSNWLDTPTLVEIRS
                     VSPDTQKSQKQNVFTLGVNVKSPKTEEDSSPTGTVSGGSQQ"
     gene            32561..33211
                     /locus_tag="PKL42_00935"
     CDS             32561..33211
                     /locus_tag="PKL42_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HNU65661.1"
                     /translation="MKLEDFNNIDFKNAGSLPGAVKAVLLLILFLVIFAVGYWYFWTE
                     AFDALEAEKVKEQELRTVFLDKKAQAINLEGYRQQMVEIEKTFGALLKQLPDKSQMDG
                     LLTDINQAGLARGLEFELFKPGQETQTEFYAEMPISIRVLGNYHDLGAFATDISKLSR
                     IVTLGDISIIASSKEGKQATNANLIMEATAKTYRYLDASEIAAKKKADSEALAKKG"
     gene            33274..33762
                     /locus_tag="PKL42_00940"
     CDS             33274..33762
                     /locus_tag="PKL42_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HNU65662.1"
                     /translation="MIGVISAGLFACSGEQGDDLDEFMKNAGNDMQARIKPLPEVKPY
                     LALQYNADNSLSDPFKARKATTKASGILPNLSRPKEPMEAYPLESIKYVGHLSKGKLT
                     YALLRTPDSGIQQVKIGNFIGQNYGMVTQITDSEVVLKEIVQDDLSGDWVERVTSLTL
                     QE"
     gene            33889..>34137
                     /locus_tag="PKL42_00945"
     CDS             33889..>34137
                     /locus_tag="PKL42_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HNU65663.1"
                     /translation="MWILFATFMGLSQAEDQSALANKIESVDFSTLPGGRVSIRVKTT
                     EPLVNAPAGFTLSDPARIALDFPKVGNGLAKNNITAGQG"
BASE COUNT         9365 a         7217 c         8025 g         9530 t
ORIGIN      
        1 cgtaatcagg taatcttgag ccacagtaga agcgacttgc tcttccgcgc ctttccaaac
       61 ctttagttgg ctgatggttt gcccttgttt ataaacggta ctggtgtcat aaaattgaaa
      121 gccataattt aatagctttt ggctttctga agcgcgtgcg gcatcatttg cagcaccaag
      181 caccactgaa acacggcgaa cgccatcacg cttggctgag gcaatcaggc aataaccggc
      241 actttccgta tgacccgttt tcatgccatc cacatttggg tctagccata gcaaccggtt
      301 gcgattttgc tgcttaattt tgttgtaggt atattctttg actgagtata agcgttggta
      361 atcttcagga aaatcatgaa ttaacgcgcc cgccaacaat gccaaatctg ccgcagtggt
      421 gtaatgctgt gggtcaggta gccccgttgc attgacaaaa tgcgtatttt tcatgcccaa
      481 acgttgcgct tctttgttca tcatgtcggc aaattgtgct tcagtactcg caatgccttc
      541 ggcaagcgca atagaggcgt cgttgcctga ttgagtaatc atgccatgta acagctcatc
      601 aacggttact ggaatgcggg gctcaataaa cattctagag ccttcaacct tgagtgccgc
      661 ttcactgaca ggcaatgttt gcgtcaattt gagatgacca tttttaagcg ccttaaagct
      721 taaataagct gtcattattt ttgttaaaga ggcgggctca acgcgcatat tgccttgctg
      781 ctctgcaata ataaatccac tattcaggtc tttgagtaaa tatgctttaa cggccaaatt
      841 gggcgcgggg gcaatttgcg cttcggccgc ctgtgtataa ggcacaaatg tagaaagcag
      901 caaagccgtg gcaatctgag ttttaaaagc gagtttgaaa cccagagatt ttttttgtgt
      961 agaaatttgc attttatgaa ataaatctat tgttttttaa cgataggcgt aatacttaat
     1021 tgcttacgaa gattgctggc agctaagtcg gcctcttttt ttgtggcata tgggccaagt
     1081 ttgacttggt acaagccgtc attatacacg ttagtcacgc tggcattgcc tgctacgccc
     1141 agtctttgaa ttttcttctg taattgttgg ccattggctt cacttctaaa tgcacccgct
     1201 tgcacataat agcctggctt gctggcgcta ttttcttgtg tagcaggcgc ctgagcagga
     1261 ggtgtaatgt cagctactgg tgcagcggca acggtttttg gctcacttgg cgtaatcact
     1321 tcaggcactg gtttgacggg aggtgcaact tctaccggag ttgctactac ctctggctcg
     1381 ggcatgatag ggcttggttc agcaggttta ctgacagggg tcgcggcggg tttcacctcg
     1441 ttaactgggg cagttttact tgtggcagca atcacggctt cattgttgct ggcgttaatc
     1501 agctccacct ctacaagacc acttccttgt tcaatcagtc ttagtttgta agctgcggca
     1561 taagataagt caattaatcg atcgtttttg aatgggcctc tgtcgttgat acgaacaatc
     1621 accgagctac catttgctgg gtttgtcact ttagcgtaac ttggcagcgg caaagtggta
     1681 tgtgcagcgg tcatggcata catgtcgtac acttcacccg atgaagtttt tttgccatga
     1741 tagcgtttac cataccatga agcgacccct tgttttttgt aaggtttgta gctggttaat
     1801 ggcacgtagt ttttacctaa agcagaataa ggcttgttag aacgctctag tggaggctca
     1861 ttcgtaattt gtgcgtttgg aatactgtcg atgtctgctg gcgcaccttc acctggacca
     1921 tcatctaaat aataaccacc agcaccaggt tttgctgtgg aggtgtcaga agattgagat
     1981 gtcggtgcgc taggttcttt agaacttggt ttagtggtaa cgccgccaca agcagaaagt
     2041 aaaataatgc tgaataaaat agtacatttt tgtaggttgg ctttagttag atgcatggag
     2101 atctactttc attggttgtt atgagacact aagtgcctac taaattttta tgtgtttgaa
     2161 tgctcattaa tataccaaaa cttacacaga gcgtcaccat cgatgtgcca ccgtaactaa
     2221 taaggggaag cggcacgcca acgacgggta aaatgccgct caccatgcca atattcacaa
     2281 aagcatatgt aaagaatgtc agtgccagac tgccagccag tagccgagaa aatgtatttt
     2341 ttgcctcaga cgcaatatat agaccgcgta aaataattaa actaaatagc gataataaca
     2401 atagatttcc cattaaacca aactcttcac taaatacggc aaaaataaag tcggtgtggt
     2461 gttcaggtaa aaagtctagt tgtgtttgcg taccattgag ccatcctttg ccattaaagc
     2521 cgccagagcc cacggcgata acagattgga tggtgttata acctgcgccg agtgggtctt
     2581 gaatgtcacc taataaaatt tcaatgcgct tcctctgata cccatgaaga agaccatgat
     2641 ggatgaaaaa atcaagtatt ggttggcgca tgatatagag taagggtgtt gccactgcta
     2701 gggtggcagc ggaacctgcg agcagtttcc agtttaaccc tgccaaaaat aaaatgtaaa
     2761 accccgaggc aaaaatgagt agggatgtgc ctaagtcggg ctgtatttta attaacccaa
     2821 ctggtatcac gagcagtaat gtggccgcag caaaatctct accatatggc ctgccttcac
     2881 atttggcgaa ataccaagca agcatcatgg gcatggcaat acgcatgagt tccgagggct
     2941 gaattttgat aaaacctaaa ttaagccagc gttgggcacc tttactacta gagccaaaca
     3001 aagcaacggc aataagcaag ataacgccta aaatataaag tggtaccgca agacgctgca
     3061 actggtgggg agttaaatta gccacaatcc acatacaccc caatgcaacc gtaatattca
     3121 cggcctgttt atacatataa gggacagatg cactgtagac aacacttaag cccagtaata
     3181 atgtgacgag tagacacgtc attaaaaatg catcaatatg cttgcctatt atttcaaata
     3241 gcctgctaat catgaaactc ttcttccatt atagactcgg catctacagc gttttctgct
     3301 ggcacattag cagtatcttt aacagaatcc acctcaggta gcttgttgag caaatagtaa
     3361 tccatgactt ttctcgcgat tggacccgct gcagagccgc catgtccacc gttttccaca
     3421 atcaccgcca gtgcgattgt ggggtcatcc gctggggcat aagccacaaa taacgcgtgg
     3481 tcacgatggc gctcattaat ggcgttggcg ttgtatttag cattttgttt gatgccaatg
     3541 acttgtgcgg tacccgtttt tgcagcaacg ctgtaaggcg catttgcccc tacgcttgct
     3601 gccgttccac ctggcagcgt aacatcaatc atgccttttt taacgatttc caaattatca
     3661 ggatgaattg taatcttatc ctgaatgacc tcaggaatga tgacggtttt cccagtgagc
     3721 ggattttgca tttttgttac taagtgtggt ttcattgcta caccgctatt ggccaatatg
     3781 gcggtgccat aggcaagctg catcggcgtt gcgagcgtgt agccttgccc aatacccgtg
     3841 atgactgtgt caccttgata ccaaggctgt ttgtaacgcc gttttttcca gtctggtgtg
     3901 ggcagtagtc cgccattttc tccttgaata tcaattccag ttttttttcc gaagccaaaa
     3961 tgactgacga aatcacttaa gtttccaatg cctaattcca tcgccaaacc gtaaaaaaat
     4021 gtatcgcaag agatcgtaat ggctttttgc atattgacga taccatgacc acttggcttc
     4081 cagtctcgat agcggtgtgt actattgggc aagctgaagt aacctgcatc atgaattgcg
     4141 tagggcggcg cacgtaaccc atgttctagg ccagcaagag cgacgaatgg tttgaaggtt
     4201 gagccgggag gatacacgcc gcgaatagga cggttgataa gtggcctgtc aatcgaatca
     4261 tttaaggctt tccagttatc aacatcaata ccatcaacaa ataaatttgg gtcaaaattg
     4321 ggctgactga caaaacttaa tacttcgccc gttttgggat tgagtgcgac taatgcgccg
     4381 cgccggtctc caaaggcttt ttcggcgatt tcttgcaatt tagtgtcgat ggaaagaaca
     4441 atattttcac caggtacagg cgctgtgctc gataatacgc gcacagctcg gccgtcttta
     4501 tcgatttcaa tttgttgaaa cccagtttta ccatgtaatt gctcttcgta aaattgttct
     4561 ataccacttt ttccgatgtg atctgaaccc ttgtaatttg aaagtacccc cggattttct
     4621 ttgtcagctt cttcaagatt cgccacgtct ttatcgttaa ttcgtccaat ataacctaca
     4681 atatgcgcgc caagtttacc caacggatag cggcggtaaa ggcgcgattt aatctcaaca
     4741 ccaggaaatt ggtatttgtt tactgcaaaa ctagcggcct cagtttcgtt gaggtgtgta
     4801 cgaataggga tgctttcaaa atttcggcta gcttgcctaa gttttttgaa tttctttttg
     4861 tccaactcac tgatttcaat caaatgactg agctcattga tggtatcttc aatattggta
     4921 tctaattttg atgggataat ttcgagcgta tagacgaaaa aattatgtgc catcactacg
     4981 ccatttttgt ctagaattaa accacgagtg ggaacaattg gcaccactga aatgcggttg
     5041 ttttcagcaa gcgtttgata atgctgatgt tggatgactt gcaaataaaa caagcgatag
     5101 gcaagcagta aaaataaggc aaccaccaca aacccagcaa acaccagtcg gcgcttgaag
     5161 tagtattgct catgttgaaa atttttgagt tcagaaagca tattaacgat gtggcgctaa
     5221 atctttgctg tgaatatcag accgaatgtg cgagaccgcg ccttggtgca acataaggtg
     5281 aacatgaaaa ttaagtgcca cgggcgcgac cacctgtatt caaaccaaat aatacggcta
     5341 atctccacag caaaatgcca gtaaagcttg gtaaaaaata ggaccagccc cacacttcag
     5401 ccccagaaaa cactttaaga ataagcaaag tgaattgtga aatgagtaac aaaatgaaca
     5461 cgtataacag ttgttgtgta tggttaaaga gtacaaggcg gcgctgatag accaccccaa
     5521 aaaaagcagt gacagtatag gctaaggcgt attgaccgaa tataccacca ctcgccaaat
     5581 cagcgatcaa acccacaagc caagcggtac caatattgca taaattgggg gcacgaatca
     5641 gccaaaaaat aaccactagt agcagaaagt cagggcgcgc ttttagcgct aatcctgacc
     5701 aaggaactag caggcaaatg agtgccacta gtagtgaaat atagactgtt ttgaagttat
     5761 tgtgtggcat tgggtttaat aggcttttca gtcaaatttg ctgctttttt tattttagtt
     5821 tggccacttt cctttttgtt tgatgttggc aatgtattct ggctagtttc gacctcttgg
     5881 ctggggactt cgtttttgat tggcatgctg acaatcagta catgacgatg gttttcaatg
     5941 ccgcctgtag gtgtgcaagt gatgcgtgca taggctgagt caggcgttgc agtgatttta
     6001 gtgacagttg caactgcaag gccagccggg tatacgccat caatgccaga agtgacgagt
     6061 ttgtcaccag tttttatatc cacattggct ggcaagtagg ggatatcgag cgtgttatca
     6121 cgaccgcggc cgaaggcgat ggcacgtaaa ccattgcgtt ctatctgcac gggaattgcg
     6181 agtgctttat cggtaatgag agtgacttca ctgctgtatg gaaagacgcg cgtcacttga
     6241 ccgataacac cagttgcatc tactactgcc tgacccgcaa taatttgatg ttgagtgccg
     6301 cgattaagga tatttttttt tctaaataag tcccgaccga cgtatatgac ttcgcccagc
     6361 acgctcgttt cagccaacgc ttgattggtt tttagcaaac ctctgagatg actattttct
     6421 agcgttaacg tattgagttt ttgtagtgca acgttatttt ctagggcttg ttgccgcaat
     6481 tgcttgttct cactcaccaa acgattttgc gtggaaaaat actcggtgac attttggtat
     6541 agctgtgaag gtgcattggc gatcacctgc aaagggtgca gtatcgcgac gaggtgttgg
     6601 cgtacggtcg ataggtattg caggcggcta tctgtagcaa ttagcgctag tgataatgcg
     6661 gaaaaaaata cgaggcgggc gaacgggctt ggcccccgca caaaaaaggc aggttcttgc
     6721 tgttgttcaa gtttgtgatt aaaccctagc gccatctttt tagacgcccc atcgtttaga
     6781 tgtcatagtc aagtattcaa taccagattt cattacatta taccaatctc aaatatccag
     6841 catgatgcaa atgtggcatt agagtgccga gtcctcagaa ataaattagg ccgcataaaa
     6901 cggcctaact tcgcatgcac ttgagtgatg tagaggcttt ctaaagtaga gataacttat
     6961 tcgtaagcaa aaatgctgcc tagtttatcc atttcatcta atgcgcggcc acatcctcga
     7021 acaacgcaag tgagcgggtc ttcggcgaca atgacaggca agccagtttc ttcaactaac
     7081 aatcggtcta aatcacgtag taatgcgccg ccgccagtga gtaccatgcc tttttcagca
     7141 atatcagcac ccagttctgg tggggtttgc tccagtgcag atttcactgc acctacgatg
     7201 gtgtttaacg gttcagtcag ggcttctaat atttcgttgc tagatattgt aaatttacgc
     7261 ggcaagcctt ctgcgaggtt gcgacctgta acttccattt cgagcacttc tgaaccaggg
     7321 aaagctgagc cgattttctt tttaatggcc tcggccgtag gttcggaaat ttgcatgccg
     7381 taattacggc gaatataatc aataatggat tggtcaaact tgtcaccccc aacgcgctca
     7441 gacttagcat aaacaatacc gcctaatgag attacaccga cttcagtggt gccacctcca
     7501 atatcgacca ccattgagcc ggttgcttca gatactggca tgccagcgcc gattgcagca
     7561 gccatcggct cttcaattaa aaatacttgt tttgcacccg cgcgttctgc agactcttta
     7621 atggcgcgac gctccacctg tgttgaaccc actggcacac aaataatgat acgagggctc
     7681 ggtgaaaacc aacgcgcatc atgtacacgg cggataaagt acttgagcat ttgttctgta
     7741 atggtgaagt cagcaattac gccatctttc attgggcgaa tcgcttgaat gctggcgggt
     7801 gcgcgaccga gcatattttt tgcttcagca ccgaccgcga gtaaaatttt cttaccccct
     7861 ggcccgccct cttggcgaat tgccaccact gaaggctcat ctaacacgat accttttccg
     7921 cgagaataga tgagggtatt ggctgtgcct aaatcgatgg caatgtcatt tgaaaagtag
     7981 ctagttaaaa aaccgaacat gatgattttc ctaagggttt gtgcaagctg acgactaaat
     8041 tttggcgtgt aatttaagcg gtcataaaag cagagtataa taccgcaaaa cgcgttgaaa
     8101 attaagatgc gattgtaagt taattgtaac tttgggattg ttgtaaatga agcgtttatc
     8161 acaaactaaa ttgcaaataa tttgcttaaa attaattttt caccacgctg tcaattagac
     8221 cgccccaata agattttgga aaatttagag catggcactg agtattgaag atattaaaaa
     8281 agtggcgcat ttagcgcgtc tagaaattag tgatgcggat gcatctgcaa cactcactaa
     8341 attgagcgga attttaaggt tgattgagca aatgcaggcg gtggacacca ctggcattgt
     8401 gcccatgtca cattcgcaag atttaagtca gcgcttgcgt gacgatatcg tcacaaaaac
     8461 caatcagcga gaagcatttc aaaaaaatgc acctgtgtta ggtaatggct caacagagtc
     8521 agccgtggca gagggtctgt atttagtgcc taaagtaatt gaataatatg atgaatcgta
     8581 gcttaaagca gtttggagaa atgctccaaa ccaagaaaat ttctagcgta gaactcacgc
     8641 aaactttttt ggctcggatt gcacagcata atccaagtat taacgcctat attgcgttag
     8701 atgaagccaa gtcacttgca caggcaaaag ctgcagatgc gttaattgcg gctggtaagg
     8761 cttcaccact cactggcatc cccattgcac aaaaagatat cttttgtgcc aaagattggc
     8821 ctacgacttg cggctctaaa atgttagcta actttgttgc gccttacgac gcgcatgtga
     8881 ttacacagtt tgatgccgtt ggcgccgtca atttaggcaa aaccaatatg gatgaatttg
     8941 ccatgggttc atcaaatgaa accagctatt ttggcggtgt aaaaaatccg tgggatgttg
     9001 aacgtgtgcc gggcggcagt agtggtggca gtgccgccgc ggtggcagct aggctatgtg
     9061 cagcagcaac aggcaccgat acaggtggct cgattcgtca acctgcttcg ctttgcggat
     9121 tttctggcct aaaaccaacc tatggcgttg tttctcgcta tggcatgatt gcatttgctt
     9181 catcgcttga ccaagccggc cctatggcaa aaagcgctga agattgtgcg ctgatgatga
     9241 acgtgatggc agggtttgat gaacgcgatt caaccagttt gaaccgtgaa aaagaagatt
     9301 acactcggga tttggcaaaa cccttgcaag gcttaagagt gggtttgcca aaagaatttt
     9361 ttgctgaagg gcttgatgca agtgttggca aagtcattga tgaggccatt gcggcgtaca
     9421 aaaaattagg tgctgaaatc gttgaaatct cactgccaaa tactgggctt tcgattccgg
     9481 tgtattacgt cttagcgcct gctgaggctt ctagcaattt gtcacgttat gatggtgtgc
     9541 gttatggcca tcgtactgcc gaatataccg atttaatgga catgtactgt aagagccgtg
     9601 ctgaaggctt tggtgcagaa gtaaaacgcc gtattttaat tggcacctat gtattgagtg
     9661 cgggctatta cgatgcctat taccttaaag cccagcaaat acgccgtttg attgcacaag
     9721 attttgttga ggcgtttaag cattgtgatg tgattatggg tccagccgca ccttcaactg
     9781 cttttaaagc gggtgaaaag gtggacgacc ccgtggcaat gtatttacag gatatctata
     9841 ccatctccac caatttggcg ggtttacctg gcatgagcgt gccggcaggg ttcagcaatg
     9901 ggttgcccgt agggttacaa attatcggta attactttga tgaagcacgc atgctgaatg
     9961 tggcgcacca atatcaacaa gtaacagatt ggcatcttaa aatgccacaa ggaattgaat
    10021 aatggaatgg gaagtcgtta ttgggttaga aacgcatacc caattaaaaa caaacaccaa
    10081 aatattttcg ggcgcgtcca cggcctttgg cgccgagcca aatacgcaag cctgtgaggt
    10141 gagtattgca ttgccgggcg tgttgcctgt gttgaaccgt gaagcagtaa attgtgccat
    10201 taaatttggc ttggccgtga atgctgaaat cgcgccacgc agcgtatttg cccgcaagaa
    10261 ttatttctac ccagacttgc ccaaaggcta ccaaatcagc caatacgaat tgccggtggt
    10321 gggcaaaggt gcggtaacga ttcaagtgcc agcagtcggc aaaaatccag cttatgaaaa
    10381 agtggtgaat attacccgcg cgcatttaga agaagatgcc ggtaaatcag tacatggcgc
    10441 agtggaaggc atgagcggta ttgacttaaa ccgtgcaggt acgcccctat tggaaattgt
    10501 gactgagcca gatatgcgct ccgcggctga agccgtggcc tatgccaaaa aattgcacga
    10561 actcgtgcaa tggataggta tttgtgacgg caacatgcaa gaaggcagct ttagatgtga
    10621 tgtaaacgtt tctgtgcgtc caaaaggcac agaaaaactc ggtacgcgcc gcgaaattaa
    10681 aaatttaaac tcgtttgagt ttatgcaaca agcaattgag tacgaaacgc gttggcagat
    10741 agaaacgcta gaagatggcg gaacgattca acaagccaca gtattgttca acccagatac
    10801 tggcgaaaca cgttctatgc gtagcaaaga agaagccaac gattatcgct actttcctga
    10861 cccagatttg ttgccacttg aggtgacaga agctgataaa gcccgtgtaa aggctgaaat
    10921 gaaagaatct caagaagcaa ttaaaacacg ttttgagaca caatatggct taaacgctta
    10981 cgatgttaat gttttaaccg cttctcgggc taaagctgat tattttgaaa gagttgttat
    11041 aggttgtggg gatgctaagt tagccgcgaa ttgggtgata ggtgagttgt ctgctctagt
    11101 caatcgcttg gatttttata gcgagaatcc gcttgctgac aaccctcctg tagatgctaa
    11161 tgatcttgcg caattgctca atcgctataa aggcggaaat ttaaataggg cacaggcaaa
    11221 agaagtatta gttgatatcg caaatcagtc ctttagtaat ttgggggaaa ccgtagatca
    11281 gattattgca agacgtggtt ttgttcagat atctgacagt ggcgcaattg aagcgatcat
    11341 tgatgaagta ttaaaagcta accctgccat ggtagaagaa tttaaagcag gcaaagaaaa
    11401 agcatttaac gcacttgttg ggcaggcgat gaaggcttca aaaggcaagg ccaacccagc
    11461 gcaagtgaat gaaattctga agaagaaatt aagctaaaat aaaccaaaaa agcacttaaa
    11521 atttattgca accttttttg atgctctgga agccttataa ataaaggctt ccagagcatt
    11581 atttttttct cggaggaatt ttagcttgtt ttgaatgttt tttttgggtg tgaaataagt
    11641 gcgtttttat gaagtagtta gttaaatcaa agcgcccaat tttataaagc tattctttca
    11701 ttttagcctg agtcgccata atttctaaat tgagccgggc aatttcttca ttggcctctt
    11761 gaatgccgaa cgacacacgc tgtggctgtt caacacctgc ttttttggca tccaacgctt
    11821 ctttgagcaa accattcaaa cgcgtttgcg tggctgcttt ttgggcttca agatctgcca
    11881 gtcgtttttt gccctcaatg acgacaggcg atttatcgtt attcggtttc agttcggcat
    11941 atcttgcctt gtaactggca aagcgcgcca tggtcacggc gatgtttttt tctactgccg
    12001 taatttcagc ctgtgttttg gtaatttgtg cctgattata tttaatgtca tcgagaatat
    12061 tgtttggtac tggtttttta cgttttagaa agccctcttg tttttctttg tttttttgca
    12121 aggtaacgcg cgtatcgtta agccgctggt taaggttagc aagcgctaac tcatcgcttt
    12181 tgatattgcg gtcacgggct aaatcaattt catcaatact gccgtacgaa gccagcaagg
    12241 ctgaatctcg tcgagattgg ctggtgttgg tgcgttcaac ttcaccgctg tcggtttttt
    12301 cgttgtaggc ttcatttttt ttaaccgtca cgcccgtttt gttgatttca ctattactgc
    12361 gccctgctgc ctctgcgggt agcttgtcac cataatgggt gacgccattg gcatctttcc
    12421 atttgacaat tttgccttca gcgctggctt gtgggctgat ggtgaagatt aaaaacaggc
    12481 acgcaatcca tagtagactg cgtgatgcac aggcgaacag gttttgacga cgtttaaggt
    12541 cgtccatgtg tagcgcgtta atttttatat gttcaggttg taaaagcaat agcttcatgg
    12601 tgccaaaaat ggccttattt ctctgttaag ggtaaaaaat ttcattgtta tcatagcgtt
    12661 aatttaatgg tatcacacca tattgttgac gatagcgctg aactgcatgc aaatattgcg
    12721 ccaattctgg tttatcttgc aaataattta gcaaatcact taaagtggca atcgcacaca
    12781 cgggcaattg atgttgttgc accacctctt gcacggctga caattcacca agcccttttt
    12841 cttgtcggtc gatggcaata gcaacgccgc aggccgtggc accgttgact ttgatcagtt
    12901 cgacagactc gcgcacagag gttcccgctg aaatcacatc atctatgatc aacacgcgac
    12961 ctttaagtgg gctgcccaca attacgccgc cttcaccatg atctttagcc tctttgcggt
    13021 tatatgcata agggatattg tgaccatttt tggcaaatgc aatggcaata cttgccacta
    13081 aggtgatgcc tttgtaggcc gggccaaata gcatgtcgaa ggcgatgccc gagttttgga
    13141 tagcggccgc ataaaattcg cctagtttca tgaggctttc accgtcatta aataaacctg
    13201 cattaaaaaa atacgggctg ttgcgcccgg cttttgtttt aaattcgcca aagcgtaaaa
    13261 cctctttttt aaccgcaaat gcgataaact cttggctgaa ctgtttttct gccgctaatg
    13321 ttttgtcttg ttggctcata ttaaaaatat tgattgaaaa ttaaaggaaa attaagtttt
    13381 gaagattata tcgctaaacc taaacggcat acgttctgca agcaataaag gtttgtatgc
    13441 gtggttaaaa aaacaagatg ccgatatcat ttgcctgcaa gaaattaagg cgcaagcggc
    13501 agatatgacg ccagaaatgt tgaatcctaa aggatatttt ggttattttc actatgcgga
    13561 aaaaaagggc tatagcggtg tgggcatcta ttgcaaacaa aagcctgatc gtgtgattga
    13621 aggcctaagc catgcgcacg acgatgtgtt agatattgat gcggaagggc gctatattga
    13681 agcgcgattt gataatttaa gcgtggtgtc gctatatctg ccttcgggtt ctagtggtga
    13741 agaacggcag gcgtttaaat ttagtgtgat gaaccgtttt ttaccacatt taaaagcgct
    13801 gaagcttagt gggcgtgatg tggtaatttg cggtgactgg aatattgcac atcaagaaat
    13861 tgatcttaaa aactttaaag gcaaccgtaa aaattcaggt tttttacccg aagaacgtgc
    13921 ttggctaagc actttgtttg atgaagtcgg ctgggtagat gtgtatcgca aattgcaccc
    13981 aaccaccact gacgaaagct atacttggtg gagtaaccga gggcaggctt gggcgaaaaa
    14041 tgttggatgg cggattgatt atcaaattgc cacaccagcc tttgcggcaa aagcggtttc
    14101 aaccagtatt tataaagcag aacgttttag cgaccatgcg ccgttgattg tggaatacga
    14161 ggatgcttaa atattagagc ttaatcgact cggccgtttt gttccatggg gcaatgtgca
    14221 acagtaacag catgccagga atcgccaata aaaagcacaa aataaagaat ttaaaccagc
    14281 ccaagctttc aaccatatag cctgtggcag cgttagcaaa cgtgcgtggc acagcagcca
    14341 agctggtaaa taatgcaaat tgcgtggcgg tataaagtgg gtgcgtggta tgtgcaataa
    14401 acgcgacaaa agcggccgtg cctaagccca caccaaatgc ctcaatacca atcacaatgg
    14461 ctaaccaaac cagccgttct tgcagattga actgatagcc taaaaagttg aactcaaaaa
    14521 aagcattaaa tagtgcgggg tcggtggcgg ctctggcaag ccacgcaaag cctaaaattg
    14581 ccaccatttg cacaatgcca aatagccaaa gcgagcgatt gatgccgagc ttaatcatcc
    14641 aaacgccacc gagtaagccg ccaatcacac tcggccaaag cccggcattt ttggcgatta
    14701 agccaatttc agttttacta aaacccatat ctaagtaaaa cggtgttgcc agcgcagtgg
    14761 ccatgctatc accgagttta tacagaaaaa taaaggctaa aatgagtagc gcagtttgca
    14821 tgccgttacg gccgatgaat tctttaaacg gctccaccac ggcagcacgc agcgtgcgcg
    14881 gcgcaccttt tttaagcatg ggctcgctga ccatcaatgt catgatgatg ccaggcagca
    14941 taaataatgc ggtaatcata aacacatgat tccatgttag gtggtcggcc aaaatgagcg
    15001 acagtgaacc cggcaccaac ccagcgattt tataagcatt cacatggacg gcattcccta
    15061 gccctaattc atggtcaagc agcagttcgc ggcgataggc atcaagggca atatcttgcg
    15121 tggcagaaaa tattgcaaca ataaaactga aaattgtaat tgggtgcgat aagaatgttt
    15181 tgacggtaga ccaaaccggc aagctgaggc tgaacaagcc attaaagtaa gcatggctct
    15241 caaaaactgg cgccgttagg tttttggctg aaacactacc aaaccaaaat acactggcca
    15301 ataaaaccag ttgggtgagt aaaatccaac cacggcgacg gccaagcggc aaagtatagc
    15361 ggtcaagaat gggcgcccaa agaaatttcc aggtgtaggg aaacagaatc agcgccatta
    15421 atccaatggc tttgaggcta acgccttcgc tacgtagcca tgctggtaac aggctgatta
    15481 aaataaaaag cggcaggcct gagctaaaac ccgtaaaaat gcatatcagc atttttttgc
    15541 tgaaaagcga ttgccatacg gtgggctgag ccgtgtctac tgtattcacg atgaagcttt
    15601 aaggcggtac atagcaagat tgcctaacag attaggcata aattcaaccg cttgagtgtc
    15661 ggtaatgacg cggcgttcca gcaccgtaat attgtgattt ttacaaaatt ggtcaaaatc
    15721 gctaatagtg caaaggtgta cattaggtgt atcaaaccat tggtaaggta gcgttttaga
    15781 cacgggcatg ttgccggtga ggatttgcaa acggttgcgc caataaccaa agtttggaaa
    15841 agtaacgatg acttcgcgcc caacgcgcag catttcttgc acaatttctt cagtatggtg
    15901 catggcttgc aaggtttgcg agagaatgac cgtatcaaag gattggtctt caaagccaga
    15961 taagccgtct tctaagtcca tttgaatgac atttgagcca ttttgcatgc aggctagcca
    16021 cttggcatcg tctttttcaa cgccatagcc tttgacctca aggctgttgc gtaaatattg
    16081 taataattcg ccatcgccgc agcccaaatc taacactttg gtaccaaatt tgacccaatt
    16141 tgcaataatt gcaaaatctt ggcgaaattc gcttacataa tttgtattat ttacattatt
    16201 ttgcatcatt gccaccaatc tcaatgttgc ttaaataagt gcgcaaaata ttgtggtaat
    16261 gcgcgtcagg cattaaaaat gcgtcatggc catgcgcagc ggtgacttca gcgtagctta
    16321 ccgtaagctc gttatccagt aaggctttaa cgatttcgcg cgagcgggca ggcgaaaagc
    16381 gccaatcgct ggtgaatgaa accactaaaa aatgcgcttt tgcctgcttt aaggcggcgg
    16441 ttaaatcatt attaaaaggt agcgccgggt cataataatc taaggcgcgc gtcatgcgta
    16501 aataggtgtt cgcatcaaat tcgccggcaa atttgtcgcc ctgatagcgc aggtacgatt
    16561 ccatttcaaa ctctgcatca aaactatatt gaatatcatt tttgagttta cgaccaaatt
    16621 tcgcgcccat ggcgtcatca ctgagataag taatatggcc taacatacgt gcgatacgta
    16681 agccgcggcg aggtattgca ttatgcgcat aatagtcgcc accgtgaaat tcagggtcgg
    16741 taataatggc ttgccgcgcc acttcattaa atgccatgtt ttgcgcggta aggttgggcg
    16801 ccgaggcaat gaccaaagcg tgacgcacgc ggttaggaaa agccaacgtc cattgtaagg
    16861 cttgcatgcc ccctaaactg ccaccaatca cggcggctag gcttggtaac cctaaaaaat
    16921 cgagtagtcg cgcttgcgaa ttgacccagt cctctacggt gacaatcgga aaagctgacc
    16981 cccaaggttt acctgtagcc ggattgatgc tcgctgaccc ggtgctgcca tgacagccac
    17041 ctaagttatt gacgccaatg acaaaaaact tattggtatc aagcggttta ttcgggccga
    17101 caaggttgtc ccaccagcca ggatatttat cattttcgct gtactttccc gccacatggt
    17161 gcgtaccaga aagcgcatgg caaatgagca ctgcatttga tttatcgtgg ttgagcgtgc
    17221 cataggtttc atatgctaaa tcaaaagtag gcagcaccgc accactcttt aacgtgagtg
    17281 gtgtatcaaa gtgcgcattt tgtggcgcga caataccaac ggaattattt tcaagcatgc
    17341 cgcaattata ctatgggttg gcaaaaacaa atgcgataac gctgaacaaa gcaaaggcaa
    17401 aagtgaagtg tagattgtgt taaagatgat tagaaatacc caatattttg caattaaaaa
    17461 gtgcattttt aagggcactt taagcaccaa cctttttcga tgctctggaa gccttataaa
    17521 taaaggctcg cagagcatta tttttttctc ggcgtaatat aacgcaaaaa atcaaaaaat
    17581 tattcagccc aatgtgtgct cacaatgagc ggatttttac cgatggtcac gcggttgtta
    17641 ccaccaaaat ctttgatggt ttcaattgaa acaaaccctg tgttggccat taaatcagcc
    17701 actttatcag cctgattaaa cccgtgttct aacattaacc aaccttgtgg ttgaagatgc
    17761 acaaggctat tttcaataat ctggcgaata tcgtttaagc catcttcacc agaactgagc
    17821 gcgctgatgg gttcaaagcg taaatcacct tggcttaaat gcgcgtcatt ttcttcaatg
    17881 tagggcgggt tgctcacaat cacatcaaac ggctgattgt ttaggtgctg aaaccagttg
    17941 cttaaaacaa attcaacatt atcaatgctt aaattttgcg catttttttt agcaactgct
    18001 aaagcagctt ttgaggcatc caccgcaata acgctagctt gcgggcgatt tttagcgatg
    18061 gccaatgcaa ttgcgccgct gcctgtgcca aggtctaaaa ttgatggatg ggcggctatt
    18121 ggaatttgtg ctaaagcggc ttcaaccagc gtttctgtgt caggtctagg aatcaaagtg
    18181 tcaggcgtca ccagcaaatc taggccataa aacgcgcgac cccctaaaat ataagcgatt
    18241 ggctcgccat tcaaacgacg attgagcaat gcttcaaacg ccgcgtgttg attggcttgc
    18301 agagcatcgt tttcatgtgc aatgagccat gcgcggttca cattgagcac atgttgcagt
    18361 aatagctgtg cttcaagttt cgcttcatca tgattcaatt ttacagtcgc gaatgccaaa
    18421 gcacttttaa tcgattgagt gtttggcatt gagttagtcg ctgggtttgg cggagagttt
    18481 aacaaggtaa atcttgatgt tagattttac acgcattacc tttttagctt agttatcatc
    18541 acccaaactc gccaacaaat ctgcctgatg ctccgccgat agcgcgccaa tcaactcatc
    18601 catatcgcct tcggtaatcg catcaatttt atatagtgtg aggttgatgc ggtggtcggt
    18661 gatgcgtcct tgcggatagt tgtaagtgcg aatgcgttca cttctgtcgc ccgagccaat
    18721 caggttacgg cgttcagagg cgattttact gttttgctca tccacttgtt tggctttaat
    18781 ccgtgctgcc agcaccatca tggcctgtgc tttgttgctg tgctgactac gcccatcttg
    18841 gcattccacc acaatgcccg tgggaaagtg cgtaatacgc acggcggagt cggttttgtt
    18901 aatgtgctgc ccgccagcac ctgatgcgcg gaaagtatca aggcgaatat ctgcagggtt
    18961 aatcaccaca tcactgacct cgtcaacttc aggcaaaatc gccacagtac aggcgctggt
    19021 gtgaatgcgg ccttgcgttt cggtatctgg cacgcgctgc acacgatgcc cgccactttc
    19081 aaatttcatt tttgaatatg cgccgtagcc ttcaattttg gcgatgattt ctttgtagcc
    19141 gcctacttca ccttcattgg cagaaagcac ttcgaccttc cacttttgtt tttctgcata
    19201 tcttgagtac attctaaaca ggtcacccgc gaacaaacca gattcatcgc cacccgtgcc
    19261 ggcacgaatt tctaaaatga tatttttttc atcattgggg tctttaggta acaacaaaat
    19321 ttgcaaagca ctttctacgg cttctaattt tgctttgcca gcagcaattt cttcttgggc
    19381 aaactctttc atgtcagggt cactcagcat ggcttgtgct tctttgatgt ctgcttcagc
    19441 ctgggtaaac gcgtgatatt gctcaacaat cggcgtgatt tcagcatgct cttgcgtgag
    19501 cttacggtag ttatccatgt tgttggtcac gccttcactg ctcatcaggc ggttaatttc
    19561 ttctaggcgc tcactcaaat tagcgagctt ggtcatcatt gatggtttca tcgaatactt
    19621 cttttaattg gcttgagatt aatggttaat ttggtaaatt tggcgcaagg tctgctctag
    19681 ccgcgtgtgt tcttcgccgt gcgtttggtt aagcgcgtgg ctgggtgcgt gtagaaattt
    19741 attcgtgagg gcgttactta atagctcaag cgctttctca gggctttcac ctttctgtat
    19801 cagtttcagt gctttgtcta attctgcctt acgggtattt tctgcctgat cacgtagtgc
    19861 tttaatcgta ggaataccgt cacgtttttt aagccagtgc ataaaatgct caacacgtga
    19921 agcgacaata atttcagcat cggcggcggc ctgctggcga ttgcctaagc cttcttgtac
    19981 cacttgggct aaatcatcca cggtgtataa aaatacatca tctaagtctt tgacttcagg
    20041 ttccacatca cgcggcacgc ctaaatccac cataaaaatg ggccgatggc ggcgcgtttt
    20101 aactgcacgc tcaaccatgc ctaagcccac aatgggtaac tggctggccg tgcaagaaat
    20161 gataatgtcg tagtctgcaa aatgtgcggg taactcttgc agcaaaatgg cctcgccgcc
    20221 aattttttcg gctaaggctg cgccgcgctc caaagtgcgg ttggctatcg tcatttgctt
    20281 tggtttttgt gcggcaaaat ggtcagcgca gagttctatc atctcacctg cgccaataaa
    20341 cagcactttt tgctcgctaa tgttgccaaa aatgcgttgc gctaacttaa ccgctgcagc
    20401 cgacatggaa acagaatttg ccccaatatc ggtattggtg cgtacttctt tggctacttc
    20461 aaaggtccgt tgaaaaagct tgtgcagcaa ggtgcccagt gtgccagcct cttgcgcaat
    20521 tttgacggat tgcttgaatt gacccaaaat ttgcggctcg cctaacacca tgctatctag
    20581 cccagaagcg acacggaacg catgctttac tgcttcatca ttcgggagcg tataaatata
    20641 gggctgaatc gcgtctttat ccagtttgtg atattgagaa agccagttga gcggtttttg
    20701 cggattatcg gtgctgcaat agagttcggt gcggttgcag gtggataaaa tagaggcttc
    20761 agtggcgtca tgcaaggtga gttcacgcag tgcgcccgcg agacggtcgc tattaaaagc
    20821 aacatgctct cgaatttgaa tgggggcggt ggtgtggttt acaccaatta cataaagctg
    20881 cataggtgta attttctcgt attggctagg gttaagcaag aagcttattt ttttgtttgc
    20941 gctttgcaag cagtgtaagc aatcttttca ccatcattga gcgtggtttc atctccgtta
    21001 atcaccagtg aaattgcacc gctactgtag cggccagcac cgctcgaaga ttttggtaag
    21061 cttacttcat ggtctgggta aattagccaa gcttcattgc cgttgttgag catacgcaca
    21121 tgaaattgct tattcccttc acagagataa gttatagcat cttgtggggt gcgcgcattt
    21181 tcttttgaac cagtactaaa tggatttaag tttatagaac tgcacgcaga taacaatgcc
    21241 gctaaagtga tggcgaacaa tatcggtttt agatgaaaat acatggtgag gtcctataag
    21301 tttaaaaatt gagttaattt tacttggaaa taacctgtga gcgtagtgat tcagttgcac
    21361 tgccacctgc gcgcgtgagt cggtcgcgta ccgcagccca aataggcgta tcagggggga
    21421 gttctactaa tagttgattc acttgtaagg catctagctc atgaagcgtg cgataaagtg
    21481 cttcagcaac ttcttgtggt tgattgatga gccgcatgat tttgcaagaa ggcttatcac
    21541 catcaccaat taacagcgct gcacaggttt gatttgcaag ctgtattgcc tcgcatgcat
    21601 gcacaaggtc agccctgtta ttaaacaaat acactgaagt gcgtggcgaa taatgcaaaa
    21661 gatgttgtcc aggaactttt ggtgttagtg tatcaaggtt gtttcggttg gttttatcaa
    21721 cattcatcgg tggtctacct gcaacaagtg cgatttcagc ctcgctaatc ataccgggcc
    21781 gtaacaattg ccaattacca ttagatgcaa cgctaacaat ggttgattca atgccaacag
    21841 cacatgcgcc gccatccagc acaggcacgt gcgttcctaa tcccatctca acatgctcgg
    21901 ctgttgttgg acttaattgc gtaaataagt tggcagatgg cgcggcaaca cttaagccac
    21961 ttaactcaag taaagcaagc gtggttgggt ggttaggcac ccgcaaagcg actgttgcct
    22021 cattaccgcg cagcacgcgg gacacatgat cttgtgcggg caatactaaa gtcagtgggc
    22081 cgggccagaa ggtctgagct agcttcacgg ccgtttctgg aaaatcagtg acccacgaat
    22141 acacttgatt aatattgcca atgtgcacga ttaaaggatg gtcagcaggc cgctgcttag
    22201 tggcaaatat cttttgcaaa gcactatcgt tgctggcatc agccgccaaa ccatagaccg
    22261 tttcagtggg aattgcgaca acttcgccac tttttaatat tgttgatgct tgcgctaaac
    22321 ttacgtgcat aacgatttta accattgccc aaagcgccat tttactactt actgcttgtt
    22381 tacaatatta aatcccctga tatcgataat atttgcatga ctatcagtta aaataacgct
    22441 tcttaattaa ctaaagaatt attcatgaat caagtggctg ttaatcaaat tactgttgaa
    22501 aataatcctg cacaaagtcg cttagatgcg ctgggcgtaa gcaagtggcc cacttggcaa
    22561 aaagaagtgt ctactttttc gtggacattt ccagagcaag agattgctta tattttagac
    22621 ggtgaatgtg tggtgacgac ctgttgcggc aatgtagtga gttttggtaa aggtgattta
    22681 gtgacctttc cagcaggcac caaaatcact tgggaagtca aacagccgct acataagaat
    22741 tatcagttag acggcacttt aattggccgc atttaccgac gcatcaaaat tgcactaaaa
    22801 ctttaggttg gacacaatat gttactcatg atcgacaatt atgattcttt cacctacaac
    22861 ctcgtgcagt atttcggcga gcttgggcaa gacgtgcaag tttatcgtaa cgatgagatt
    22921 gatttaaaaa aagttgcagc gctaaagccg cgccatattg taatttcacc tggcccatgt
    22981 acaccgaatg aggcgggcat tagcgtgccc ttaattcacg agttcgctgg caaaattccg
    23041 ctactgggtg tttgcttggg acatcaaagc attggccaag cctttggcgg caaggttatt
    23101 catgctaaac aactcatgca tggcaaaact tcactgattc atcacaaaaa tatgggtgtt
    23161 tttaagggct tgcccaatcc ttatacggca acacgctacc attcattggt cattgagcgt
    23221 gaaactattc cagattgttt agagattacc gcatggaccg atgatggcga gattatgggc
    23281 gtgaaacata aaacgcttgc ggtagaaggc gtgcaatttc atccagaatc tatcctgact
    23341 gaatttggcc atgaactgct ggataatttc ttaaaagctt attaactgag gttttggaca
    23401 tgttcaaaca ggcgctcaac caactgctcg cgcgtgaaga tttaagtttc gacaccatgc
    23461 tgtcggtcat gcaacaagtc atgggcggcg aacttacgcc tgcacaaatt gccgcccttt
    23521 tggtggcgtt gcgcatgaaa ggtgaaacgg tcgaagaaat atccgctgcc gcaacggtga
    23581 tgcgcagcct ttctaccaaa gtggatattc aagaccatgc ttatttggtc gatacctgtg
    23641 gcacaggtgg tgacggtatc caaacgttta atgtatctac ggcctgtgca tttgttgccg
    23701 cggccgctgg tgccaaggtg gcgaagcatg gcgggcgctc ggtgtcgtcc acctgtggca
    23761 gtgccgatgt tctggaggtg ttgggggtgg atgtgaatcg cacgcctgcg caagtggcaa
    23821 acagtgtgaa tgcgattggt atcggcttta tgtttgcgcc aaatcatcat agtgcgatga
    23881 aacacgcggc acccgtccgc agagaattgg gcgtacgcac aatttttaat ctgcttggcc
    23941 cacttaccaa cccggcttca gccaagcgac aagtgatggg cgtgtttgat aaacacttaa
    24001 caactaaact tgcacatgtg ttgcaacatt taggtgctga gcacgtattg gtagtgcatg
    24061 gtgaagatgg catggatgaa atttcatttt caggcgacac atttgttgct gagttaaaaa
    24121 atggcaaaat ccatgaatac acgctgaacc ccgcgcattt tggtttgccc catcatgacc
    24181 tcagccaaat tcaagttgaa aatgctgaac aatccaaagc gattatttta gatgtgctca
    24241 acggtaaacg gggtgctgcg cgagatattg tattgctgaa tgcgggtgca gcaatttatg
    24301 tatctggcgc agttgatagc ctagctgacg gtgttaaaaa agcagcagaa gtcattgata
    24361 acgacgctgc gttggcaaag cttgaagcgc ttaaaaaaca agtagcagca tgaaaaagta
    24421 tttatttctg tttatcttgt taagtgcgct ggttgcttgt aaacaaaata ccagcagctt
    24481 aagcgctggc aatacagtac cagccgtaca aagtgaggat aaagggttgg cggtttcgcc
    24541 aaatgaaaat gcctcgctta tcgaagcttt tcaacacaaa aaaagcaatc tttttgttga
    24601 aggccgcgga gtaataaaga agttgctgcc agatgacaac aagggctcac gccatcaaaa
    24661 atttttagtg acgatttcag atgcgcaaac attattgttt gcgcacaata ttgatttagc
    24721 caaagctgta cccattgctg tgggtgatac ggtgcaattt agaggcgaat atgtgtataa
    24781 cccaaaaggt ggtgttgtgc attggacgca tcatgaccca aaagcaaaaa tcgagggcgg
    24841 ttggattaaa cacaacggtc aaatttacca ataagcagaa acgattatgt ctgacatctt
    24901 aaataaaatt ttagctacca aacagcaaga gattgccaaa agtaaggcag tggttagctt
    24961 agcgcaatta caaaaacaag ctgaggcgca aggcgaccca cgtgattttg taggaaatat
    25021 ctttgccaaa gtcagcgcga acaaaccagc ggtgattgcc gaaatcaaaa aggccagccc
    25081 ttcaaaaggc gtgattcgtg aggattttaa ccctgcagaa attgcaaaga gctatgaaaa
    25141 aggcggcgcg gcgtgcttgt cagtactcac tgacgttgag tattttcaag gcagtgccga
    25201 gtatttaaag caagccagaa aagcctgcaa actgccagtg ttgcgcaaag actttatgat
    25261 agatccctac caagtatttg aagcaagggc gatgggtgct gactgcattt tgctgattgt
    25321 ggccgcgctc gagttgaatc aaatgcgcgc cttagagcaa cttgcccatg atttaggcat
    25381 ggcggtgctg gttgaagtgc atgatgccga tgagttgaac atggctttgc aacttgatac
    25441 gcccttaatc ggcatcaata accgcaattt acgcactttt gaagtgaccc tgcaaaccac
    25501 attagacttg cttaaagtgc tgcctgaaga ccgttttgtg gtcacagagt caggtatttt
    25561 caccccagaa gatgtgaagt taatgcaaga ccatgatgtg catgccttct tggtgggcga
    25621 ggcgtttatg cgccagccgg acccgggcgc cgagttggca agagtgtttg gcctttaaat
    25681 aattaatgaa agtcatgtcg aggtaaatcg tatgagctat ccatattccc gcccacgccg
    25741 cctacgtaaa gatgaatttt cacgccgttt ggtgcgtgaa aatgtgctga cgaccaatga
    25801 tttgatttat ccggtgttcg ttttagatgg cgaaaatcgc gttgaagagg ttgcatcaat
    25861 gccaggtgtg aaacggcaaa gtatcgatat tttgctcaaa accgccgcag agtgtgtgca
    25921 actgggcatt cctgcaatcg cgttatttcc ggtggttgat agccaattta aaagtttaga
    25981 tgcagcagaa gcctacaacc cagaaggctt ggcacagcgc gcagtggcgg cgcttaaagc
    26041 cgcttatcca acgctgggcg tgattactga cgttgcgcta gaccctttca ccacgcatgg
    26101 ccaagatggc ttgattgatg attcaggcta cgtgctgaat gacgaaacca tcgaagtgtt
    26161 agtgaagcag gctatttcac atgccaatgc gggtgctgat attgttgcac caagcgatat
    26221 gatggatggc cgcgtggggc aaatccgtga agccttagaa agctcaggca acattcacac
    26281 caaaattttg gcctactcag ctaagtatgc ctctgccttt tatgggcctt ttagagatgc
    26341 cgtgggttct gcgggaaatt tgggttcagg caataaatat acctaccaaa tggaccccgc
    26401 caatagtgat gaagccatgc aagaagtcgc gctggatatt agcgagggcg cagacatggt
    26461 gatggtgaag ccggggctac cgtatttgga tattgtacgt cgtgtaaaag atgagtttgg
    26521 tgtgccgacg tttgcctacc aagtaagtgg cgaatatgcc atgctaaaag ccgccgcgca
    26581 aaatggctgg ttggatgaaa aggcatgcgt gatggagagt ttgttggcgt ttaagcgcgc
    26641 gggtgcagat gggattttga cttattttgc gatggatgta gcgcggtggt tgaaggttaa
    26701 agattaaaat cttttatgaa taatgaacaa ataagattca tgtatgagaa atacatggat
    26761 attgtaaaac ttgaacttga agttgaagag tttggatgta gaccgacaga agtgcgtcat
    26821 ttaataggta ggttaggtga gttcttttgt gcaatacatg ttaatggaaa gcttgctcac
    26881 gaggcgaatc aacatggatt tgatgttata gccgagaacg gtaggcgaat aagcgttaaa
    26941 acaaccgcac aaaaatcggg ttttataaca attaacccga atacaattaa caaagttgat
    27001 gacttaatgc taattcaatt tatagattct agacttgaag ttgtcttcca tggtccaatt
    27061 caaaaagcag tagatgtatc tagaacttgg gaaggtaaat tcgaatttga tatttcaaaa
    27121 gccaaaaagc taacgtaatt ttcttgatta agattacttt tcgcctttgg gcgagtttct
    27181 ttcttttgct tagtcagaaa gaaagaaacc aaagtaacac ccgcaagggg catccccaac
    27241 ctcactaagt gctaaagcac taagtcggtt gtgacagccg cttaccgaaa ccctcccaaa
    27301 atcaacacaa tctcaggcgc ggtagccggg gaaatttggg tgaagattgt tagaaaattg
    27361 gctctatttt taagctataa aaccgctgtt ttaagtcaca acctttttcg atgctctgaa
    27421 agccttataa ataaaggctt ccagaggcat aaaaaatcct cgggcgtttt tagggttgtt
    27481 ttaagtgttt gcgatgaggt ttttgacggt gctaatatct gcgcgttctg cattgctgcc
    27541 gttggctgcg cgtttggtgg gtacgcgtat ttcatcaaat tcaaatacac tgagtttgat
    27601 gatgccagtg attgtttcga gtgaaaaaat cggcactttt ttgcgtgcat ccattttttt
    27661 gtcgcgtttg ccatcattca gaatgcggta ggatttttcg ttggtttcaa acggaatatc
    27721 ttggttcagt aaaaacattt ccacttcttt gaggctgtcg gcaaataagt gaatgtgcgt
    27781 ttctgtgcct aatcctgcgg tgccatctag caccgcaccg gttaaatagg ggttgaattt
    27841 ttcgagcagt tgcatggtaa agagcgcatt tttgcgtaag gcgcgcaggt tttcaggttg
    27901 cgcttcactt aaaaacaacg cgttatagcg cttgagttct tcttcaattt cagtgtttga
    27961 aggcagtacg ctgttatcga gcgcgccaat ttgccgccct gctttatttt tggcgtatga
    28021 gaaatccgag atgccgtctt ctgccatcat ttgcgcggct ttttgagcaa tcagttgccg
    28081 caattgggtt gggttttctt tcgccatggt tgcttgctcg tagcatttaa ttcaatttgt
    28141 atttaacatt tttgcagcag cattttgtgg atctgcgttg ctattttctg gctttgcttg
    28201 attagcagcg ttgttaggcg gcgataaaac aacctcaggc tgtaatactt tttgtgcctg
    28261 attttgttgc gcatttgcat cttctccgtc agtttcagca ttatcatctg ttaattcctc
    28321 ttcaccgaat aaaggcggga gcggctcttc gtgtgttgga ggcgggtttt catggtagaa
    28381 gtattctaaa atgccgctac tgctacgggt gccagtcgca gggtcaatgt taatggccac
    28441 aaccccttca ggcatgcgca tggccatttc tggcacacca tttagggcca cggccatgta
    28501 cttaatccag ataggcaatg cggcggcacc gcctgtttca cctctgccca tgttgcgcgg
    28561 cttgtcaaaa ccaatccaag caatccctac ctgctttggg ctgtagcctg taaaccacgc
    28621 gtctttgtgg tcattggtgg tgccggtttt acctgcaatg tcggtgcgtt taagtgagag
    28681 cgctttggtg gcggtgccgt tttgaacaac actttgcagc atggagttca taatgaacgc
    28741 attgcgtgca tcaatcacgc ggggcgcatc tttacccgcg cgctgatatt ttgattgcat
    28801 aatcaccttg ccgttactat caacaatttt ggtgatgatg ttcggtttga ccaaatagcc
    28861 accattggca aagaccgcgt aggcagttgc catttcccag ggcgttgttg aacccgcacc
    28921 taaagctagc gtgaggtaag gtgggtggtc tttcgcttta aagccaaatc gtgttagata
    28981 gctttgggcg tagctggggc caatcttttc aagtatgcgg atagctacag tattaatcga
    29041 ttgcgttaac gctttcctca ggcgtacagg gcctctgaaa tcgttgtcat agttttgtgg
    29101 tgaccacgca tcatcacccg tttcggccgc agaaaagctc agcggtgcat catccacaat
    29161 agaggcggcg gtgtatcctt tttctaacgc cgccgagtaa ataaacggtt ttaagcttga
    29221 gcccggttgg cggtgcgcct gtgtgacatg attaaatttg tttcgactga aatcaaaacc
    29281 acccacaagt gcacgtactg cacctgtttc tgggtctatg gcaataaatg cagattcaac
    29341 ttcaggcagt tgcacaacgc gccatgcctc agccaaattt gtaccgtcac tctttttagt
    29401 tgcagtctta atgacacgaa tcacagcgcc tggcttcaat aagcgttttt caggatcttt
    29461 ttctttgagc gttttttcaa ttaatttcag cccgttacca gtaacttcaa tgtcatcgcc
    29521 atacttggta tgggcaaata tagatttagg cgtgatttta gtaataattg caggaattaa
    29581 atttgcatac acttcaaatt catctaaagc atcatctaat gcagttttat cgtccctgga
    29641 agttatcgcg tcgagggtca atattttttc aggtccgcgg taaccgtgac gcatgtcgta
    29701 atcgagcaca ccttcgcgaa ccgcattgtt tgcggcgagt tgattagctt tgcggatcgt
    29761 cgtatagact tttaaaccgc tcgtgtatat ttcatcctga tatttcgcat acaaattttc
    29821 gcgcacaatt tcagcaacat aatcagcaga taaactacta cgttcttgtt tagcactttt
    29881 aaaacttaaa ggctgtttta gcgcctcttg ataagcttct tcttttaaaa agccaaatct
    29941 aagcatatcg cgcaacacct catgctgccg accaatggcg cgtttagggt tggtaaatgg
    30001 attgtatcca gaaggtgcct taggtaatcc tgccagcaat gcgatttcag caagattgag
    30061 tttttcaagc ggcttgtcgt aataagcttg agccgcagca gcaaaaccat aggcacgttg
    30121 gcctaaataa atttgattta agtacagttc tagaatttta tccttactta aattccgctc
    30181 aattttaacg gcaagtaatg cttcgttaat tttagtaata atcgtgcgct taccatttgg
    30241 tggcgtaaaa aagtttttgg ccacctgcat ggtaatagtg ctggcaccct cacggccttt
    30301 accagtgata ttgtttttaa tggcacgaac aacccctgtt gtatcaacac cggcatgctg
    30361 ataaaaacga cggtcttcaa tggctaaaac ggcatctttc atggcctgag gaacgttttc
    30421 aatcttgata taagcgcgac gctcctcacc gaactcacca atcaagtggc catcctcaga
    30481 aaagacctgc aatggtaatt ttggatgata atctgtgagt gcttcaagcg atggcagtga
    30541 gggatatatg attgctgcgg ctattgctat tagcgccgca acagacatgc aactgacaaa
    30601 aattgctaga aaaatataat gccaccattt gttaagcgtc atagaattta tcagtccaca
    30661 tagtcttaag agttcgcagc attataaatt gccaagcgca attctgcgaa taaacaatgt
    30721 aattaagtat ttattttgta tcaattctgt aacaaatagg tatataaaca ttattgttaa
    30781 atgtaaagaa attaaaaatt actttacagg ggttaaagtt gttgatagga tttaaagtag
    30841 tttattagaa gtttacgtaa ctatttaact ttgagaggga atttcgattt gaaattcaat
    30901 ttgtttacag aatcaacgcc gccactcgtc ggggtggata tcagctcgac tgctgtgaaa
    30961 atggttgagc tcagtgacaa tggtcgcggc ggctacaagc ttgagggtta ttccatctca
    31021 gctttaccta aagaagcgat tgtagacggt aacattggtg accttgagaa ggtagttgac
    31081 ggcgtgaagc tagcatggaa actgttaggc tctcgagaaa aacgcgtggc gctagcgcta
    31141 cctgcctccg cagttattag caaaaaagtg ctgatgagtg ctggtttacg tgaagaggat
    31201 atggaagtac aggtagaggc tgaagccaat cagtatattc ccttctcgct agatgaagta
    31261 aacattgact ttcaggtgat aggcgcggcg ccaaataacc cagatgaagt tgaagtgtta
    31321 attgcggcag cccgtaaaga aaaaattgaa gaccgtgtag cggctgctga gggtgcgggg
    31381 cttaaagtga cggtgatgga tgttgaaaac tatgccactg aagctgcata tagcttggtg
    31441 gcaagccagt tacctaattc agggcgcgag cagacagtga tgattgtgga tattggcgca
    31501 tccatgatgc atgtcaatgt gctgcacgat aataaatcgg tctatatgcg tgatcaaagt
    31561 tttggtggtg gtcaattgac acaggaaatt caacgccgtt ttggcttgtc tactgaagaa
    31621 tcagaaattg cgaaacgtaa gggtggtttg cctgaaagtt atgagggcga agtattgcaa
    31681 ccatttatgc aagcgcttgc aactgaagtg gcgcgcgcag tgcagttttt tacgagttca
    31741 acccaataca atcgtgttga taacatactt ttggcaggcg gttgcgcagc aataccttca
    31801 gttgacgtgc tggtacaaga gcgcacccaa gtgcatacgg ttgttgctaa tccattccac
    31861 agcatgacaa tcagctcaaa actcaagcag caacaagctg caatcgacgc ccctgcactg
    31921 tttgtggcgt gtgggttggc aatgcgaggt ttgtcaatat gatacgcgtt aatctgttac
    31981 cacatcgtca ggtcaaacgt gctgaacgtc agcgcgagtt cggcctgatg gcagcttttg
    32041 cagcatttgc tgctgcggca attatcttct taggttatac cttcataagt agccaggtag
    32101 aagcacaaac agcacgaaat tcccggttag acgaagcaat agtgaaatta gataaagaaa
    32161 tcgcggacat taaagatttg aaagatcaaa tcagtattat gctggataga aagcaagtgg
    32221 ttgaaaactt acaaacgaat cgcagtcaat cggtcgttgt gctagatgaa attagtaggc
    32281 agttaccaga aggtatgtac ctaaaaagca ttaaacaaca aggtaactcc atcacccttg
    32341 agggtgtagc tgatacaaat tctcgagtgg caactttagt gcgtaatttc tcaacttcca
    32401 actggctaga cacgcctacg ctggtggaaa ttcgatctgt ctcgccagat acacaaaaat
    32461 ctcagaaaca gaatgtattt acattgggcg tgaatgttaa atcacccaaa acggaagaag
    32521 atagttcacc aaccggcact gtaagtggtg ggagtcaaca atgaagttag aagattttaa
    32581 taatattgat tttaaaaatg caggtagttt gccgggtgcg gttaaagctg ttttgttgct
    32641 tatattattt ttggttatct ttgctgtggg ttactggtat ttttggacag aggcctttga
    32701 tgcgctagaa gctgaaaaag taaaagagca agaattgcgt acagtatttc tagataaaaa
    32761 agcgcaggca attaatttag aaggttatcg gcaacagatg gtggaaatag aaaaaacttt
    32821 tggtgcctta cttaaacagt taccagacaa atcacaaatg gatggcttgt tgacagatat
    32881 caaccaagcg ggcttggcgc gcggattaga gtttgaacta tttaaacccg gacaagaaac
    32941 acaaacggaa ttttatgctg agatgcctat ttcgataaga gttttaggta attatcacga
    33001 cttgggtgcc tttgccacgg acatttctaa gctatcgcgg attgttacgc taggcgatat
    33061 ttcaattatc gctagcagta aagagggtaa gcaagctaca aatgctaact taattatgga
    33121 agccacggct aaaacttacc gctatttgga tgccagtgaa attgccgcta agaaaaaagc
    33181 cgatagtgaa gctttggcta agaaaggttg agtggtcaag tgaatataag tacaaaacag
    33241 cggatttttt tatatcgagt acctcgattg ctaatgattg gcgtcatttc ggctggttta
    33301 tttgcatgta gcggcgagca aggtgatgat cttgacgaat ttatgaaaaa tgctggaaat
    33361 gacatgcaag caagaattaa gcccctgcca gaggtgaagc cttatctggc cttgcaatat
    33421 aatgcggaca attcactcag tgatccattt aaagcccgta aagcaaccac caaagcttca
    33481 ggcatactgc ccaatcttag tcgccctaaa gagccaatgg aagcctaccc gttggagagt
    33541 attaaatacg tgggtcattt atctaagggt aaactcactt atgcattgct aagaacacca
    33601 gatagcggca ttcagcaagt gaaaataggt aactttattg ggcagaatta tggcatggtc
    33661 actcaaatta cagacagtga agtagtgcta aaagaaattg tgcaggatga tttgtctggg
    33721 gactgggtag aacgcgttac gagtttaaca ttgcaagaat agtcctttgt ttaaatagtg
    33781 ggtaaaaatt tttagtatta aagcgtttta aatatttaaa gcgacttaag aaatcagggt
    33841 gaaataatga aaacacaatt gatggttaaa tctttagcgc atgcatgcat gtggatttta
    33901 tttgcaactt ttatgggtct ttcacaagca gaagatcagt cagcgctagc aaataaaatt
    33961 gaaagtgtgg acttctcaac tttgccagga ggtcgagtta gtattcgcgt taaaactact
    34021 gagccattgg tgaatgcgcc agcaggtttt acattaagcg acccagcacg gattgcccta
    34081 gattttccta aagttggaaa cggccttgcg aaaaataata tcacagctgg ccaaggg
//
LOCUS       DAOQSI010000006        32580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_763_length_32580_cov_6.028101, whole genome shotgun sequence.
ACCESSION   DAOQSI010000006 DAOQSI010000000
VERSION     DAOQSI010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 32580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32580
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_763_length_32580_cov_6.028101"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..170)
                     /locus_tag="PKL42_00950"
     rRNA            complement(<1..170)
                     /locus_tag="PKL42_00950"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(872..2083)
                     /gene="tyrS"
                     /locus_tag="PKL42_00955"
     CDS             complement(872..2083)
                     /gene="tyrS"
                     /locus_tag="PKL42_00955"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNU65664.1"
                     /translation="MTTNTVGFNINEQLAVIKRGADELLIEAELIEKLKKGKPLRIKA
                     GFDPTAPDLHLGHTVLINKLRQFQELGHHVLFLIGDFTGMIGDPTGKSATRPPLTAEQ
                     VQENAKSYTAQVFKILKPEQTEVVFNSKWLTELGAAGMLKLAASHTVARMLERDDFSK
                     RYKNNQPIAIHEFLYPLLQGYDSVALKADVELGGTDQKFNLLMGRELQKQAGMAQQCV
                     LTMPLLEGLDGVNKMSKSLGNYIGIAEAPEIIFAKIMSISDELMWRYVELLSFESLET
                     IAQWKAQVMAGENPRNIKVRFAQEIVTRFHSQADAEKALSDFQTRAKGGIPDEVPEIS
                     ISIDAATIGIAQLLKLAGLVESTSEAYRAIEQGGVKLNSEKVTDKHLSLNKGVTVVAQ
                     VGKRKFAKITL"
     gene            2203..3597
                     /locus_tag="PKL42_00960"
     CDS             2203..3597
                     /locus_tag="PKL42_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNU65665.1"
                     /translation="MKFNESNPHNKAFNYFAKENLSSLSAQNAILTQIAHKAERKLKL
                     RWVLAISCLPLFGIYTAFGIAPQTMVGNIATAIVVEETGLPQLNHSVANAGEQHFWYK
                     ERVLRDDTLNTILSRLNVMNRDALDFIRSDSVAKEIASSLRPNRNIEAQTDADGNLIT
                     LEYQLSDGQFIAIQHTADGYEAGKAMRELESRPILKSAKINSSLFGAADAANIPEAIA
                     IQIAELFESEIDFNTDLRRGDQFKVIYEGSYYQGELIKTDKILAAEFTNNGKTYRAVG
                     FKDAAGEMQYYTPEGKSLRKSFLRSPLEFTRISSGFSLGRFHPVLQTLRAHKGTDFAA
                     PTGTGVKAAGDAVVDFVGVKGGYGNVVVLKHDNGVSTVYGHLSRFANGLQKGKKVAQG
                     EMIAYVGTTGLSTGPHLHYEFLVNGVHRDPMTVALPKDNALSGQDKLAFDLQSQQLTA
                     QLQLLGSSNLAALD"
     gene            3638..4774
                     /locus_tag="PKL42_00965"
     CDS             3638..4774
                     /locus_tag="PKL42_00965"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HNU65666.1"
                     /translation="MVGKLFIGLMSGTSLDGVDVALVEFNTFNNPHRPPHVIQTHFVP
                     YPPALRAQILALQHPTDNELEITALAGNQLARLYANAVNALLTKQALSSSNISAIGCH
                     GQTIRHQPGFKDGTGFTLQIGNAALLSELTGITVVSDFRSRDIAAGGQGAPLVPAFHQ
                     AVFSHPQKNRILVNIGGIANLTYLPKTDDVLNAVIGFDSGPGNMLMDYWTKLHLNQDY
                     DTNGQWASTGLVLDRLLADMLAEPYFALPPPKSTGRDLFHAGWLKQHLLYPHLRPQDV
                     ARTLVELTAETIFQAIVNTSLAVDEVYLCGGGAHNTLLTARLKDKLGNIPLTTTEALG
                     MGVDWVEAIAFAWLAKQTLEGKTSSLPEVTGAKGARILGAIYPH"
     gene            4970..6277
                     /gene="gltA"
                     /locus_tag="PKL42_00970"
     CDS             4970..6277
                     /gene="gltA"
                     /locus_tag="PKL42_00970"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNU65667.1"
                     /translation="MKSPTKPTKVTLSFDNGAPSIELPILSGSLGNDVIDIRSLAKHG
                     IFTYDPGFMSTAVCNSSITFINGEEGLLYYRGYPIEQLAEHCDFMEVSHLLINGELPT
                     SVQKEEFTRTINSHTMVHDQLTNIFRGFRRDAHPMAVMVGVVGSMSAFYHTKGDIHDA
                     QSREITAHQLLAKVPTIAAWSYKYNLGQPFMYPQNHLSYAENFMYMMFATPCEKYEPN
                     PILAKAFDRILILHADHEQNASTSTVRLAGSSGANPFACIAAGIASLWGPAHGGANEA
                     TLKMLEEIGDISRIGEYIKRAKDKTDAFRLMGFGHRVYRNMDPRAAIMRKTCHEVLDE
                     LGLHNDPMFKLAMALEKIALEDEYFVSRKLYPNVDFYSGIVMRAMGIPNSMFTAIFAL
                     ARTAGWIAQWSEMNADPEHKIGRPRQLYMGEARRELVPISNRK"
     gene            complement(6405..7022)
                     /locus_tag="PKL42_00975"
     CDS             complement(6405..7022)
                     /locus_tag="PKL42_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HNU65668.1"
                     /translation="MKKIICLLVILLASCSAGEKYPAIPKGATVVILGDSLSYGTGAN
                     VGEDYPSLLAQTTGWHVVNAGVPGDTATGGLARLPQLLEKHAPKLLIVELGGNDFLQR
                     QPPSETEASLRAILSLAKTQRVPTVLLAIPELSPIRAAVGNLSDHPLYENIAEETATP
                     LVAEIFSEVLSDQALKSDQVHPNAEGYRVVHREMVEAFKQLGFAR"
     gene            complement(7109..7474)
                     /gene="erpA"
                     /locus_tag="PKL42_00980"
     CDS             complement(7109..7474)
                     /gene="erpA"
                     /locus_tag="PKL42_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HNU65669.1"
                     /translation="MNTMTEIQDVEIPSPLVFTDNAAKKVKELIEEEGSPDLKLRVFV
                     SGGGCSGFQYGFTFEEEVNEDDTQVMKDTVTLLIDPMSLQYLMGAEIDYQDSLQGSQF
                     VIRNPNAQTTCGCGSSFSV"
     gene            complement(7539..7943)
                     /locus_tag="PKL42_00985"
     CDS             complement(7539..7943)
                     /locus_tag="PKL42_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNU65670.1"
                     /translation="MFFKKSNRIQDSIDTLIGADTRIEGNVHFTGGLRVDGVVRGNVT
                     EPNASPSTLILGEKGRIEGAVTASKVVIIGNVVGPVTSGIFIELQATAHITGDVYYKS
                     LEMHTGAVIEGKLVYLGDSAKAANEGIDQTNL"
     gene            complement(8000..8365)
                     /locus_tag="PKL42_00990"
     CDS             complement(8000..8365)
                     /locus_tag="PKL42_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65671.1"
                     /translation="MKPVKRKIRRHYGLTAKQVAVRSKRPWYYRWVLSACILAVGYGL
                     AYWQYAAGGKSLAEKYKLATLENQNLHTKVIQTERQLQIEQAAKTNLNQELNLVQDEN
                     IKIKEDLLFYKNMMAKKAK"
     gene            complement(8394..9452)
                     /gene="argC"
                     /locus_tag="PKL42_00995"
     CDS             complement(8394..9452)
                     /gene="argC"
                     /locus_tag="PKL42_00995"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNU65672.1"
                     /translation="MMENNKKIKIGIVGGTGYTGVELLRLLSVHPNATLTAITSRGEA
                     GLPVAEMFPSLRGYVDLKFTDPATANLNDCDVVFFATPHGVAMRQAEALLEAKVKVID
                     LAADFRLQDTAVFEKWYKMPHSCPAVLREAVYGIPELYREQIKSAQVIGNPGCYPTTV
                     LLGLAPLLEHGLIDFSAPVIADSKSGVSGAGRKAEVATLFAEASDNMKAYGVSGHRHH
                     PEIHAQLTQLAGNDVQFIFVPHLIPMIRGMLSTIYVKLSDIAKSVDLQALFEARYQHE
                     RFVDVLPAGVLPETRSVRGSNQIRIALHQHAGTDYLTLVVVQDNLVKGAAGQAVQNMN
                     IMFGLPETAGLEVVPLLP"
     assembly_gap    9544..9643
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9711..10103)
                     /gene="rpsI"
                     /locus_tag="PKL42_01000"
     CDS             complement(9711..10103)
                     /gene="rpsI"
                     /locus_tag="PKL42_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNU65673.1"
                     /translation="MIGKYNYGTGRRKSSVARVFLKSGKGNIVVNDKPVDEYFSRVTS
                     RMILRQPLELTNNLASFDIMVNVIGGGESGQAGAVRHGITRALIDFDANLKSALSHAG
                     FVTRDAREVERKKVGLRKARRRKQFSKR"
     gene            complement(10115..10543)
                     /gene="rplM"
                     /locus_tag="PKL42_01005"
     CDS             complement(10115..10543)
                     /gene="rplM"
                     /locus_tag="PKL42_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNU65674.1"
                     /translation="MKTFSAKSHEVKRDWFVVDATDLVLGRLASAVAHRLRGKHKTIY
                     TPHVDTGDYIVVINADKMKVTGNKADGKLYHSHSGYPGGISTTTFSKMQDKFPGRALE
                     KAVKGMLPKGPLGYAMIKKMKVYAGDKHEHAAQQPQALKI"
     gene            complement(10660..11541)
                     /gene="ubiA"
                     /locus_tag="PKL42_01010"
     CDS             complement(10660..11541)
                     /gene="ubiA"
                     /locus_tag="PKL42_01010"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HNU65675.1"
                     /translation="MNLQQLNKKINAYERLMRLDKPIGILLLLWPTLWALLIAAQGKP
                     DWIIVLIFITGTVLMRSAGCVMNDLADHPFDGLVERTKHRPLITNEVSKKEAYWLAAG
                     LCLAAFGLVCLMNRLTIYISFAALFFAATYPLTKRFLAIPQAYLGIAFGMGIPMAFAA
                     TTGNVPTVVWLLMAANIFWAIAYDTEYAMVDRDDDLKIGIMSSAITFGRFDVLAVMLC
                     YAATLLLLVIAGQQLALGYGFYGGLGVAAVVAIYHYRLIKAREKSQCFKAFLHNNWFG
                     FAVFVGLVAHYFTHYFM"
     gene            complement(11538..12086)
                     /locus_tag="PKL42_01015"
     CDS             complement(11538..12086)
                     /locus_tag="PKL42_01015"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HNU65676.1"
                     /translation="MKIQQHYSSERSRWLRKPFASGAFVSWLIETGSLTARLQRRYAN
                     FAVQTVRLEDAKPLDDEAGLLQSKPHKRALVREVLLLGNQQPVVFAHSVLPQASLRGA
                     WYGLGKLGNKPLGATLFANPKVKRTPLSYKKLLPNHALYRLAASHLADKPAYLWARRS
                     IFHLNCANILVTEVFLPQLLDQ"
     gene            complement(12377..14416)
                     /gene="recG"
                     /locus_tag="PKL42_01020"
     CDS             complement(12377..14416)
                     /gene="recG"
                     /locus_tag="PKL42_01020"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNU65677.1"
                     /translation="MTALSTLKTFSKPLLASLNKLGISQLQALLLHLPLRYIDETHIT
                     VVRDLRLGEAALVEGEIVHMEVTYKPRKALIARLEDASGQLTLRFLHFYPSQIAMLKE
                     GSHLRVYGEVRHGYFGYEMVHPTCKKVGEKTEVAETLTPVYPTVAGLTQASLRKAISI
                     ALVHNGLHETLPASVYAKLNLPSFAASLKALHHPPPDADLQALENKTTLEWQRLAFDE
                     LLAQQLSMRKHYARRRSVNAPAFAPSKKLVSSLLKSLPFALTQAQQKVAVEIERDLTQ
                     HHPMQRLLQGDVGSGKTIVACMAALQAIENGWQVALMAPTEILAEQHYQKMLGWLTPL
                     NISIAWLTGSQTKKEREAALQAIAQGYAQMVVGTHALFQEAVQFKKLGLAIIDEQHRF
                     GVQQRLALRKKGEPEPHQLMMSATPIPRTLSMSYYADLDVSVIDELPPGRTPIVTKLV
                     SDERREEILQRVREACLAGSQAYWVCPLIEESEALQLATANDTYALMQSEFPELKIGL
                     IHGRMKPAEKQAVMAQFSANTLQLLVATTVIEVGVDVPNASLMVIEHAERMGLSQLHQ
                     LRGRVGRGATKSTCILLYQNKLSETARARLKVIYESNDGFAIAQADLNLRGPGEYLGT
                     RQSGVPMLKIADLNRDADLLQAAKNCADDLIQQYPAAVEKHLERWLASAADLVKV"
     gene            complement(14469..16772)
                     /locus_tag="PKL42_01025"
     CDS             complement(14469..16772)
                     /locus_tag="PKL42_01025"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNU65678.1"
                     /translation="MPKTAKHSQADTSKADLENSALAILAQAPLLPADSEDSALTILL
                     KQYLKPQDVTLVREAYRFAFVAHDGVVRKTGEPYITHPVSVACILADLHMDVPTILAA
                     LLHDVVEDTAITAQEIKDKFGAQVAELVDGVTKLDKIEFQSASQAQAENFRKMLLAMS
                     QDVRVILVKLADRLHNMQTLTAMKPESRKRIAKETLEIYAPIANRLGLNLIYQELEDL
                     SFKALYPMRYRVLTQVIKAARGNRKEVISKILESIQSKLKEAGIEAEVTGREKHLYSI
                     YKKMAGKTNSFSQIYDIYGFRVVVKDLPTCYLTLGALHGLFKPIPGKFKDYIAIPKAN
                     GYQSLHTTLFGPFGTPIEVQIRSIDMHNVANAGVAAHWLYKSSDAELTALQQQTHQWL
                     QRLLEIQSESVDSLEFLEHIKVDLFPDEVYVFTPKGKILALPRGSTAVDFAYAVHTDV
                     GNSCVAVKINQELAPLRTELKNGDHVEIITAALSKPNAAWLNYVKTGKARSHIRHYLK
                     TMQSEESTHIGERMLNQALRAMHVEPSAVKEKQWEKLIRDYSVKNKQDILTDIGLGKR
                     QSVMVAHQLLAIDDKQPTDETVGVVSKLFGKLFGKANKKPLNTITIRGSEGMSVQFAS
                     CCRPIPGDPILGFINKDKGLIIHTHDCPAIRKFSLDPEKWLDVEWDSDSKRLFKVNLK
                     LVAENKPGMLAMIAAIIAEAGSNIDNVSMEESDNSVYTGLNFTVQVRDRVHLAELMRA
                     LRKVPNVVRINRAKGNSTANGTQQVSQ"
     gene            complement(16797..17003)
                     /gene="rpoZ"
                     /locus_tag="PKL42_01030"
     CDS             complement(16797..17003)
                     /gene="rpoZ"
                     /locus_tag="PKL42_01030"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNU65679.1"
                     /translation="MARITVDDCLDQIPNRFQLTLVAAYRARQLHNGAEPLINTQGLR
                     DKTTVLALREIAAGKVGVEILQPR"
     gene            complement(17042..17653)
                     /gene="gmk"
                     /locus_tag="PKL42_01035"
     CDS             complement(17042..17653)
                     /gene="gmk"
                     /locus_tag="PKL42_01035"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNU65680.1"
                     /translation="MTTGNLFIITAASGAGKTSLIKALLAKDSQLKLSISHTTRKPRA
                     SEIDGVDYHFVDDATFLSMLGEARFLESAQVHDARYGTSQAAVEGPLKAGFDLVLEID
                     WQGAEQVRRLFPQATSIFVLPPSIEALERRLTGRAQDSAEVIAKRVAAAREEMRHVSA
                     FDYVTINDDFDVALQDISAIIRAQRLRGMAQLQRYASLIQSLT"
     gene            18078..19886
                     /locus_tag="PKL42_01040"
     CDS             18078..19886
                     /locus_tag="PKL42_01040"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNU65681.1"
                     /translation="MDKKLTPLELARQTLIQLSKSQSPPTPENYRRVYDEIAGIKSVD
                     SVDVLGKSLEKVLTELGKDRPKYLATSQKIAAFIKKRDAENLEDQLRKLMPTSANEAD
                     GVNWPTLLRYLLKQLDVNHTGTTLSRKKEGLNRVIINFANDPSQLGEKIQALITSWGD
                     GQPSASTNSATALQAESTLIVEVASATQTNAALTESEQALTTAWRDLLTRAIKLVVLP
                     QFADIPAASDKIENVIQLAQKANTVEEINALGETLKSFILRAEMRNDAQHRMQESLVQ
                     MLRLLISSMGELTIEDKWLHGQISIVQDIISKPLDIDAVYNAESSLKELIYKQSNIKP
                     GLIAAKETLKGMMSSFVSGLADISRSTGTYQAKINQYQVQISETEDITQLNTILQNLV
                     GDIREMNADAQNSHNAFEETQKKVEEAEKQINELSAKLDYISEVAHQDFLTGALNRRG
                     MDEAIDREFERADRHNTPISLAMIDIDHFKKINDTMGHSTGDTALAHLAKVVKSILRS
                     TDVLARYGGEEFVILLPGSKQNDAVNVVAGIQRDLTKNFFLHNNERVLITFSAGVAER
                     VTGENIDSVLPRADAALYIAKQSGRNRVVGAEPLNN"
     gene            complement(19909..20775)
                     /locus_tag="PKL42_01045"
     CDS             complement(19909..20775)
                     /locus_tag="PKL42_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNU65682.1"
                     /translation="MTYSMTGFAALEKPIVNATLLLELRAVNSRYLDLHFKLDENLRG
                     FEPTIRELISAQLNRGKIECKVNLIQRTQLSQEAALDGALMQQLAVMQSQAKAYFPTS
                     RELSMADILRWPGVVLTQGIDQDLLLEDIKQLVQQGLQDLKASRAREGDKLKKLILDR
                     IHQMEALVEKVKPLLPTLAKEYQAKLENKLQETLKSIDPERIAQELVLFTQRVDVDEE
                     LSRLTAHVSEVKRILNGDAPAGKRLDFLMQELNREANTLGSKSVSTQTSQVSMELKVL
                     IEQMREQVQNIE"
     gene            20984..21958
                     /locus_tag="PKL42_01050"
     CDS             20984..21958
                     /locus_tag="PKL42_01050"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNU65683.1"
                     /translation="MSSTKNLALPSGYQLQDYKIDKVLSSGGFSFVYLAHDKDNKLVA
                     IKEYLPTSIALRSNGATVMPNPDDAALFRHGLKCFFDEGLSLAKIDHRNIVRVINFFR
                     ANETVYMVMQYEQGKSLQDYILGQTELVSEIFIRQVFSELAKGLREVHAQKLLHLDIK
                     PANIYIRLDESPVLLDFGSARQALNENLAKSSPSYTPGFAPPEQYFDRNLLGPWSDIY
                     SIGATMYSCLTRTSPAAANLRVKNDLLVPAVKLGLSNYSQSLLEIIDQCLSLDYLARP
                     QSVFSLQKSLLKSMPSPKKKSRLDKIVGVLKSPVSDVFNTPIISKKSQ"
     gene            21955..22881
                     /locus_tag="PKL42_01055"
     CDS             21955..22881
                     /locus_tag="PKL42_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNU65684.1"
                     /translation="MKFTIFQNSLQGPRQYNQDRLAYSYSKDALLLVVADGMGGHRHG
                     EMAAQLAVTTMTEAFQRLAVPTLTSPAKFLIDHIQQIHDMIDQLTQEREMLESPRTTI
                     VAAIVQRGMLYCAHVGDSRLYHFRNNHLLYRTEDHSVVQSLYNKGIINKSEMTTHPYR
                     HKVYSCLGGDTPPKIDLSDRQELAEGDTILLCTDGVWGALTDDEIKVMLYGPSITDNV
                     TALLNKAELVSQAQGDNMSAIGLQWGDKLSDPFAVSTITMAMGATTTIMNPVKRNILG
                     SDDTFTDDFSDDDIENTINEIQRALQKNKSAP"
     gene            22952..23698
                     /gene="rph"
                     /locus_tag="PKL42_01060"
     CDS             22952..23698
                     /gene="rph"
                     /locus_tag="PKL42_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HNU65685.1"
                     /translation="MQANTSNLENLRPSARANNQLRDIEIIRKYTKHAEGSVLVKFGD
                     THVLCTASVDEKVPGFLKGKGQGWVTAEYGMLPRSTGSRMDREASKGKQSGRTQEIQR
                     LIGRSLRAIIDLEKLGERSIQIDCDVIQADGGTRTASITGAYVALADAINHLLKIGKL
                     QNSPLKQAVAAISVGVYKGQPVLDLDYIEDSDCDTDMNVVMTADGGFVEIQGTAEGEP
                     FDRAAMNAMLDLAAHGIGQLVIKQQEALNV"
     gene            23691..24290
                     /gene="rdgB"
                     /locus_tag="PKL42_01065"
     CDS             23691..24290
                     /gene="rdgB"
                     /locus_tag="PKL42_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNU65686.1"
                     /translation="MFKKLVIATGNQGKLREIRHILGELDIEIVPQSALNVPECEEPF
                     CTFIENALTKARHASKHTGLPALADDSGLCVDALLGAPGVLSARYAGEPKSDERNNQK
                     LLEVLGSENNRHAHFYCVMVLVRHEHDPEPIIAEGQWHGEILNEYRGTDGFGYDPLFL
                     DAKTGKTVAELPLEIKSRISHRGHAMAKLLQKLERLSHD"
     gene            24283..24507
                     /locus_tag="PKL42_01070"
     CDS             24283..24507
                     /locus_tag="PKL42_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65687.1"
                     /translation="MTKPSHTVWPKWLRDDGSVVSCTEKVKVMTENFDEIKQITQDAL
                     EDGLLMEVSEQQMREALHQLVDSLINPYKR"
     gene            24601..25761
                     /gene="hemW"
                     /locus_tag="PKL42_01075"
     CDS             24601..25761
                     /gene="hemW"
                     /locus_tag="PKL42_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNU65688.1"
                     /translation="MSGLTDSPPLSLYIHIPWCVQKCPYCDFNSHESRHQHGEIPEST
                     YVDALIADLEQATPKVWGRKIKSVFFGGGTPSLFKPASIDRILSHVRALTPLDYDAEI
                     TLEANPGTVDIANFIGYKQAGVNRLSLGIQSFNAKHLKALGRIHTDRQAIHAAELALT
                     TFERVNFDIMYGLPNQSMTHALEDAKQAIALNPDHLSFYHLTLEPNTPFYRSPPILPD
                     DDTCAEMQVEIENLLAEHGYEHYETSAFSKPGKQAKHNLNYWQFGDYLGIGAGAHSKL
                     SYHDKITRETRPKHPKAYMEQSLLGNAADNIWNISDNELGFEFMMNALRLTDGVPVNL
                     FQMRTGLHINTLEGSITQAQNKKLVVVKNDYIKPTLLGQRFLNELLTLFMTN"
     gene            complement(25787..27256)
                     /locus_tag="PKL42_01080"
     CDS             complement(25787..27256)
                     /locus_tag="PKL42_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65689.1"
                     /translation="MKLNTHSLRINIIIFLASLVFSNYAYAEEFLYEDAYSNATEYTL
                     ESTSTPWSLLAGESLSDLARLLYPNSKQMQQRFISKSLQLSRHIQPNLNPSNRADQMT
                     VIIIPNIKFLGTGSAKSKDSRTNLVGKQAKNYASQDALAPNATQQKAYDYLLIKNTFL
                     KEELIKLNAKLLALVELLASLKIELMRLLAQVDIQSAQVPSTASAGESSIESAALGHH
                     VAGSVTNASPKAAKYLLQPILTVLFVLSLFLALAFFNQQQKNFLNPRNVYLRAKRSYG
                     NMKAELFNDNSFLKTKRSPTIFASDFPLEPVVKSEYSGSMIVTDLSDVGIPHFEDDAA
                     QALEQAKIFLAIQREDEAIKLLKAQISAAPKATLQHWLLLLDIYRKTNQKAEFMQYAK
                     LLHENFNVMMPSWDSSTLPSAINSSLEAFAHISTKMTQLWANCEKEAQKISQTKSYLD
                     ELLLDNRNNERSGFSFEVFKEIILLRDMLDVREKLEFAD"
     gene            complement(27379..28809)
                     /gene="argH"
                     /locus_tag="PKL42_01085"
     CDS             complement(27379..28809)
                     /gene="argH"
                     /locus_tag="PKL42_01085"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNU65690.1"
                     /translation="MTEKLSALNKKDTSWSGRFNEPVAELVKKYTASVGFDYRLALYD
                     IQGSIAHAQMLSEQGIISREDFTAIEKGLIEIKAEISAGKFDWRLNLEDVHLNIEKRL
                     TDKIGDAGKRLHTGRSRNDQVATDVRLWLREITLQVIEGLSRLQTSLLDLAEAHFDTV
                     MPGFTHLQVAQPITFGHHLMAYVEMLKRDVARFHDCYKRINQLPLGAAALAGTSYPIN
                     RERVAKLLGFDGVCENSLDAVSDRDFAIEFTFASSMVMMHLSRFSEELILWSSPRFAF
                     IDIADRFCTGSSIMPQKKNPDVPELVRGKTGRVYGHLTALLTLMKGQPLAYNKDNQED
                     KEPLFDTADTLLVTLEIYADMLRLPLIENGVVTKAGFTVNKDNMRQAASEGFATATDL
                     ADYLTKKGMPFRDAHEVVALAVRYAVDHNVELSNLSLETLRQFATEIDEDVFSVLTLE
                     GSLNSRNHIGGTAPSQVKAAIVRARR"
     gene            29007..29999
                     /locus_tag="PKL42_01090"
     CDS             29007..29999
                     /locus_tag="PKL42_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNU65691.1"
                     /translation="MRKSSRLPNLHNLGVNLRILIIVNAIIALTAIVLSNHFEDFYVV
                     ITTISSIAQPLLLLCLLVLYGSYSLLTKLRYWQGLLGIFTIVLLVCSGFYDLISHLLI
                     YSNLPSRLRLLFFASIITAICLYYFYLHQHAYSPAVIEARLQALQARIRPHFLFNCIN
                     AVLSLIRTQPKQAETALEDMADLFRVLMADNRDLVPLGQEIALCRQYLALEKLRLDER
                     LTVQWKIDDMPPDAMIPPLILQPLIENAVYHGIEPLEKGGTITIQIYTSRKEIHLVLS
                     NPYNEASLRHIGNKMALKNIKERLTLHFDLEASLKSSQSNGEYRVQITLPYKEV"
     gene            29996..30754
                     /locus_tag="PKL42_01095"
     CDS             29996..30754
                     /locus_tag="PKL42_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNU65692.1"
                     /translation="MTTMIKLLIADDEAPARKRLRDLLSDINEVTIVADAKNGQEALK
                     IANENLPDIVLLDIRMPIMDGIETAQHLQKLAKPPAVIFTTAYDTYALQAFDLNAVDY
                     LLKPIRFERLQTAIQKARALLPKQLEALVPLSPQRTHLSISERGRVLLIPVADVIYFR
                     AELKYITVKTAEKEYLLEESLNHLEQEFGKIFIRLHRNCLVAQAYILGYEKRQLSHDA
                     EGLLSSEKQWVALLKNIPDTVTVSRRQQHLIRSN"
     gene            30810..31760
                     /gene="hemC"
                     /locus_tag="PKL42_01100"
     CDS             30810..31760
                     /gene="hemC"
                     /locus_tag="PKL42_01100"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HNU65693.1"
                     /translation="MTPNTNLAPDKLVIASRESPLAMWQALHIQSRLQALYPETVVEI
                     LGMTTTGDQILDTPLARVGGKGLFVKELETALADGRADLAVHSMKDVPMNLPEGFLMA
                     ATGEREDPRDAFVSNNFASLEALPAGSIVGTSSLRRQSQLQARFPDLKIESLRGNLQT
                     RLRKLDEGQYAAIILAAAGLIRLGLQARIRSVISPELSIPAVGQGALGIEISAARTDL
                     LAVLAPLNHADTEVCVKAERAMSRALAGSCTVPLGAYATCHADAIEITGFVASVDGKQ
                     MLVETATGHRNNAEALGQQLANQLLASGAGEILAALDGHK"
     gene            31781..32557
                     /locus_tag="PKL42_01105"
     CDS             31781..32557
                     /locus_tag="PKL42_01105"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNU65694.1"
                     /translation="MTNQTLAGLHVVVTRPIGQAESLCETIRHLGGFASAFPLLAISP
                     LDDYREFDQAIAALEHADWAIFISTNAVDSAMHRVLARYGNVPESVQFAAIGHQTAKE
                     LARFGVHHVLTPHTRFDSETLLALGEMQDVANQTVIIFRGVGGRELLAETLKARGANV
                     MFAESYRRVNPQTNTDLLSHLWQHQKLDAIVVTSSEAMRNLLDLAGQSEWLRHVTLCV
                     NHARIAELPLQFGLKVLVADAPGDDAIIQRLAQLVKPHGK"
BASE COUNT         8986 a         7524 c         6880 g         9090 t
ORIGIN      
        1 ggtattagcc aacctttcga ttagttatcc cccattattg gatacgttcc gatatgttac
       61 tcacccgttc gccactcgcc accagaccga agtccgtgct gccgttcgac ttgcatgtgt
      121 aaagcattcc gccagcgttc aatctgagcc atgatcaaac tcttcagttt aattcacaac
      181 tattactttt tcctcaacca tctttcgata gccaaggtgg tatttcgctt ttgctttatt
      241 tatctataaa tagataaagt actcaaattc atttcaaggt gtgtcatatg attgtcacta
      301 ttactagtca caattctatt tacatacttg tcataaatct aagtgtaagc acttgtattt
      361 tctgaggctt agcttaagcc aatcggttaa gttaaattca atatatatat cgaacaagtt
      421 aaccgcgcct aagtaagctc gtctcaatcc acaagcaccc acacttatca gttgttattt
      481 tgttaaagag ccggtctaaa aagtattttc tttctagtta ttcgttagcg ctttgcgtta
      541 acgaggtgcg cattatacag agctttcgaa gcgcgtcaat actaatttaa aacatttttt
      601 aattaattat tgacggtctg ttttgctgca tttcgctcac ttgttttgtt agcgaaggcg
      661 cgcattttac aggggctttg aatttggtca agtactattt tcgcgtgtta cataattatt
      721 acaattacgt gttttaattt gctcaaaatt taggcaaaat tcgctcgaga aaaaaattat
      781 gccctggaag cctttgttta taaggcctcc agggcatcaa aaaaggttgg gtatcaaata
      841 gcgggttaat ttggtcgcta tttacactta atcaaagtgt gatttttgca aatttccgtt
      901 tacccacctg cgccacgact gtcacgccct tatttaaact taggtgttta tcagtcactt
      961 tttcgctatt gagttttaca ccaccctgct caatagcgcg gtacgcttca gaggtgcttt
     1021 caacaaggcc tgctagtttg agcaactgcg caatccctat ggttgcagcg tctatagaga
     1081 tgctgatctc tggcacctcg tctggaatgc cgcctttagc gcgcgtttgg aagtcgctca
     1141 atgctttttc tgcatccgct tggctatgaa agcgtgtgac gatttcttgc gcaaaacgca
     1201 ctttaatatt gcgagggttt tcgcccgcca tcacttgcgc tttccattgc gcgatggttt
     1261 ccaagctttc aaatgagagt agctcaacat agcgccacat taattcatct gagatggaca
     1321 tgatttttgc aaagatgatt tcgggcgctt ccgcgatgcc tatatagttg cccagtgatt
     1381 tggacatttt gttaacgcca tccaaacctt ctaacagcgg catggtgagt acgcattgct
     1441 gcgccatgcc agcttgcttt tgtaactctc ggcccatcaa taaattaaac ttttggtcgg
     1501 taccgcccag ctcgacatcg gcttttagtg caaccgagtc gtagccttgc aataatgggt
     1561 ataaaaattc gtgaatggca atcggctggt tgttcttata gcgcttagaa aagtcatcac
     1621 gctctaacat gcgcgccacg gtgtggctcg ccgccaattt aagcatgcct gctgcgccta
     1681 actctgtcag ccatttggaa ttaaacacaa cttccgtttg ttctggcttg agaatcttaa
     1741 acacttgtgc cgtataagac tttgcgtttt cttgcacttg ttcggccgtc aagggcgggc
     1801 gcgttgcact tttgccagtg gggtcaccta tcatgccggt gaagtcgcca attaaaaaca
     1861 atacgtgatg accaagctct tgaaactgcc gcaacttatt aataagtacg gtgtggccca
     1921 agtgtaggtc tggcgctgtt gggtcaaaac ctgcctttat tctgagcggt ttgccctttt
     1981 ttaatttctc aatcagctct gcttcaatca atagctcatc agcaccgcgc ttaataacag
     2041 cgagttgctc gttaatgttg aagccaacag tattagtagt cacgtatgag tatccttaaa
     2101 ccatatagtg caaaacatga actgttacag ttcatttact tgtttaaatt gagttttctg
     2161 ttattatcca caaccgtatt aaacttttac agaggtcagc ccgtgaaatt taacgagtct
     2221 aatccacata acaaagcttt taattacttt gccaaagaaa atctatcaag tctttctgcg
     2281 caaaacgcta ttttaacgca aatcgctcat aaagctgaac gcaagcttaa attgcgttgg
     2341 gttttagcga tttcgtgctt gcctttattt ggtatttaca ccgcatttgg tattgcacca
     2401 caaaccatgg taggcaacat tgccacagca attgttgttg aagaaacagg cttgcctcaa
     2461 ctcaatcaca gcgtggcaaa cgctggtgaa caacactttt ggtacaaaga gcgcgttctc
     2521 cgtgacgata ccttaaacac aatccttagc cgcctgaacg tgatgaatcg cgatgcgctt
     2581 gattttattc gctcagatag cgttgctaaa gaaatcgctt cttcattaag accaaaccgc
     2641 aatattgaag cgcaaacgga tgctgatggc aatttaatca cgcttgaata tcaattaagt
     2701 gatggccaat ttattgccat tcaacacact gcagatggct atgaagccgg caaagcgatg
     2761 cgcgaacttg aaagtcgccc tatattaaaa tctgccaaaa ttaacagctc tttatttggt
     2821 gctgccgatg ctgcaaatat tccagaagcg attgcaatac aaattgcgga actatttgaa
     2881 agtgaaatag atttcaatac ggatttacgg cgtggtgacc agtttaaagt gatttatgaa
     2941 ggcagctatt atcaaggtga gcttatcaaa actgataaaa tcctggcggc agagtttacc
     3001 aataacggta aaacgtatcg tgccgttggt tttaaagatg cggctggcga aatgcaatat
     3061 tacacacccg aaggcaaaag tctgcgtaaa tcatttttac gttcaccgct tgaatttacg
     3121 cgaattagct ctggctttag tttaggccgt tttcacccgg ttttacaaac cctaagagcg
     3181 cataaaggta cggatttcgc ggcacctacc ggcactggtg taaaagcagc tggcgatgct
     3241 gtggtggatt ttgttggtgt taaaggcggc tacggaaacg tggttgtact caagcatgat
     3301 aacggcgtaa gtactgttta tggccatcta tcacgttttg ccaatgggtt gcaaaaaggt
     3361 aaaaaagtag cgcaaggcga aatgatagct tacgttggca cgaccgggct ttcaactggg
     3421 ccacatttgc attatgaatt tttagtcaat ggtgttcatc gcgatccgat gacggtagcc
     3481 ttaccgaaag ataacgcact ttctggtcaa gacaaactcg cttttgattt acaaagccag
     3541 caattgacgg cgcagctcca attattgggg tctagcaatc tcgctgcttt agactaaaac
     3601 aaaaatttca catctataac aattttattg tcgcaaaatg gttggtaagc ttttcatcgg
     3661 actcatgtct ggaaccagcc tagatggtgt cgatgtggct ttagttgagt ttaatacatt
     3721 caacaatcca catcgccctc ctcatgttat tcaaacgcac tttgttcctt acccgcctgc
     3781 attacgcgca caaattctag ccctacagca tcctactgat aatgaacttg aaataacggc
     3841 tttagctggc aaccaactgg cgcggcttta tgcaaatgca gtgaatgctt tgcttacgaa
     3901 gcaggcatta tcttcaagta atatttcagc cataggctgc cacggccaaa ccattcgtca
     3961 tcaacctggg tttaaagatg gcacgggctt tacgttgcaa attggcaatg ccgcgctttt
     4021 aagtgaactt actggcatta cggttgtaag tgattttcgc agtcgcgaca ttgcggcagg
     4081 cggccaaggt gcgccgttgg tccccgcctt tcaccaagca gtgttctctc acccgcaaaa
     4141 aaatcgtatt ttggtcaata ttggtggcat tgccaattta acgtatctgc caaaaacaga
     4201 tgatgtgcta aacgccgtca ttggctttga ctctggcccg ggcaatatgc tgatggacta
     4261 ttggaccaag ttacacctaa atcaggatta cgatacgaac ggccaatggg cctccacagg
     4321 attagtgcta gaccgattat tggcagatat gctggctgaa ccttattttg ccctgccgcc
     4381 gcccaaaagc actgggcgtg acttgtttca tgcaggctgg ttaaagcaac atttacttta
     4441 cccgcattta cgcccgcaag atgttgcgcg cacattggtt gaactcaccg ctgaaaccat
     4501 ctttcaagca atcgtcaaca ccagcctcgc ggttgatgaa gtctacctat gcggcggtgg
     4561 cgcgcataat acgctgctga cggcgcgttt aaaagataag ttaggtaata ttccccttac
     4621 caccactgag gccttaggca tgggcgtaga ctgggttgaa gcgattgcct ttgcctggct
     4681 ggccaagcaa acgctggaag gcaaaaccag tagcctgcca gaagtcaccg gcgctaaagg
     4741 cgcacgaatt ttaggcgcta tttacccgca ctaagcacat tgcctttagg aaacacggaa
     4801 ttaagtgagc gagcgggctg aagtgcgagg cgcacggcgc acagaaaccg agatagtacg
     4861 gggtgtacag cgaggttgcg agcaccgcgc aacgaagcaa ttcacccgcg cagacactta
     4921 actccgtgtt tcctttataa tatggcaaaa actaatcgag tattttgcta tgaaatcacc
     4981 cacaaaacca acaaaagtca ccctcagctt tgataatggc gcgccttcca ttgagttacc
     5041 catactctcc ggcagccttg gcaatgacgt tattgacatt cgcagcttag caaaacatgg
     5101 tatttttact tacgaccctg gctttatgtc tacggctgtc tgtaattcaa gcatcacctt
     5161 tattaacggt gaagaaggtc tgctgtatta ccgcggttac ccgattgagc agctggctga
     5221 acattgcgat tttatggaag tttcacacct gctcataaac ggtgaactcc cgacttccgt
     5281 gcaaaaagaa gaattcacgc gtaccatcaa tagccacacc atggtgcatg accaactcac
     5341 gaatattttc agaggattca gacgtgatgc acacccgatg gcggtcatgg tgggtgtggt
     5401 gggttcaatg tcagcgttct atcataccaa aggggacatt catgacgcgc aaagccgtga
     5461 aatcactgcg catcagttgc ttgccaaagt gccgactatc gccgcatgga gctataaata
     5521 caatttaggg caacctttca tgtatccgca aaaccatctc agctatgcgg aaaattttat
     5581 gtacatgatg tttgccacac cttgcgaaaa atatgaaccc aacccgattt tggccaaagc
     5641 ctttgatcgt attctgattt tacatgctga ccacgaacaa aatgcctcta cctcaacagt
     5701 acgcctagcg ggttcgagcg gtgctaaccc gtttgcctgt attgcggctg gcattgcctc
     5761 tttgtggggg cccgcgcatg gtggcgcaaa tgaagctacg ctaaaaatgt tagaagaaat
     5821 tggtgacatt tcacgtatcg gcgaatacat caaacgcgca aaagataaaa ctgatgcgtt
     5881 ccgcctcatg ggatttggtc accgcgtata ccgcaatatg gacccgcgcg cggcgattat
     5941 gcgcaaaacc tgccacgagg tactcgatga actcggtttg cacaatgacc cgatgttcaa
     6001 acttgcaatg gcacttgaaa aaattgccct tgaagatgaa tactttgttt cacgcaaact
     6061 gtacccgaac gtcgattttt acagcggcat tgtgatgcgc gcaatgggta ttcctaacag
     6121 catgtttacc gctatttttg cgctggccag aaccgccggc tggattgcgc aatggtcaga
     6181 aatgaacgcc gaccctgaac acaaaatcgg ccggcctaga caactttata tgggtgaagc
     6241 tagacgcgaa cttgtgccta tttcaaacag aaaataagca cttttaaccc gcaacctttt
     6301 tcgatgctct ggaagcctta taaaaaaggc ttccagagca taaatttttt ctcgtagctt
     6361 ttaatggttg cttaagtgat tttttttaga ctaaattttg ctgcttaacg ggcaaagcca
     6421 agctgcttaa atgcctcaac catttcgcgg tgcaccaccc gataaccttc tgcattcggg
     6481 tgtacttggt ctgattttag cgcctgatct gacaacacct ctgaaaatat ttctgcaacc
     6541 aatggcgttg cagtttcttc agcgatattt tcataaagcg gatggtcaga taaattgccc
     6601 actgcagccc gaattggact caactcaggg atggccaata acactgttgg cacgcgttgc
     6661 gttttagcta acgacaaaat cgcccgcaag cttgcctctg tttcacttgg cggctggcgc
     6721 tgtaaaaaat cgttaccgcc cagttccaca atgagtaatt ttggggcgtg tttttctaac
     6781 aactgcggta agcgcgctaa accgcccgta gcggtgtcac ccggcacccc cgcattcacc
     6841 acatgccaac ctgtcgtttg cgcaagtaaa cttgggtaat cctcacccac gttcgcgcca
     6901 gtgccataac tcaaactatc gcccaaaata accaccgttg cgcctttagg aatggcaggg
     6961 tacttctctc cagcgctgca agaagccagc aaaataacca gcaaacagat aatttttttc
     7021 atgctttttt aggctctaat attaaaagtt gaaattataa aaagaaaaag ccaagcaaag
     7081 gcttggcttt tgaacttatt ttgacacttt atacagagaa tgatgaacca caaccgcaag
     7141 tggtttgagc attcggattg cgaatgacaa actgtgaacc ctgcaagctg tcctgatagt
     7201 caatttctgc gcccatcaaa tattgcaagc tcatcggatc aatcagcaaa gtcaccgtat
     7261 ctttcatgac ctgtgtatcg tcttcgttca cttcttcttc aaaagtaaag ccgtattgaa
     7321 agcctgagca accaccaccg ctcacaaata cgcgcagttt taaatctggg ctgccttctt
     7381 cttcaatcag ttctttcact ttttttgccg cattgtcggt aaacaccaac ggtgaaggta
     7441 tttctacgtc ttgtatttct gtcatcgtat tcatcttgta ctcctaaaat attcaataaa
     7501 atcattatgc tactgcgtag cttgttggtc aacctaagtt agagatttgt ttggtcaatg
     7561 ccttcatttg cggcctttgc actgtcaccc aaataaacga gtttgccttc aatcaccgcg
     7621 cccgtatgca tttctagcga cttgtaatac acatcaccgg taatatgcgc ggttgcctga
     7681 agttcaataa aaatacctga tgtcaccggc cctaccacat tgccgataat cacgacttta
     7741 gaagccgtca cagcgccctc tatacggccc ttttcaccta atattagagt gcttgggctg
     7801 gcatttggtt cagtgacgtt gccacgcacc acgccatcaa cacgcaaacc gcccgtaaaa
     7861 tgtacgtttc cttctattcg agtatcggcg ccaatcaacg tgtcgatact atcttgaatt
     7921 ctgttacttt ttttaaaaaa catcacattt cctattctta aattcagtga gcgataggtt
     7981 ttgagtatta aagcaccatt cacttagctt tttttgccat catattctta taaaacaata
     8041 agtcttcttt tattttgata ttttcgtctt gcacaagatt tagttcttga ttcaagttag
     8101 ttttcgccgc ctgctcaatt tgtagttggc gctctgtttg aatcactttg gtatgcaggt
     8161 tttgattttc aagtgttgcc agtttatatt tttctgccaa acttttgccg cctgcagcat
     8221 attgccaata agcgaggcca taaccaactg ctaaaataca ggcactcaaa acccaacgat
     8281 aataccaagg ccgctttgag cgcacagcga cttgttttgc cgtcaagccg taatgtcgtc
     8341 tgatttttct ttttactggc ttcatttagc ggcttattat cggacagaaa agtttacggc
     8401 aacagcggta caacttctaa accggccgtc tctggcaaac caaacatgat gttcatattt
     8461 tgtactgcct gccctgccgc acctttcact aaattatctt gaaccacaac cagcgttaaa
     8521 taatcagtgc ctgcgtgttg gtgtaaggcg atgcggattt ggtttgagcc acgcactgac
     8581 cgtgtttcag gcaaaacccc tgctggtaat acatcaacaa aacgctcatg ctggtaacgt
     8641 gcttcaaaca acgcctgcaa atctaccgac tttgcaatgt cagacagttt aacgtaaatg
     8701 gttgataaca tgccacgaat catcggaatc aaatgcggca caaaaataaa ctgcacatcg
     8761 ttgccagcta actgcgtaag ctgcgcatga atttcagggt gatggcggtg gccgctcacg
     8821 ccataggctt tcatgttgtc actagcttct gcaaataacg tggcgacttc tgcttttctg
     8881 cccgcgcctg atacgccaga ctttgaatca gcaatgactg gggcagaaaa atcaatcaag
     8941 ccatgctcca gtagcggcgc caagcctagc aatacggtgg ttggataaca gccagggtta
     9001 ccaatcacct gcgccgattt tatttgttcc cgataaagct caggaatgcc atacaccgcc
     9061 tcacgcaaca ctgctgggca actatgcggc attttgtacc atttttcaaa cactgcagta
     9121 tcttgcaggc gaaagtctgc cgccaaatca atcactttta ctttagcttc cagtaatgcc
     9181 tcggcctgac gcatcgccac gccatgcggc gttgcaaaaa atacgacatc gcagtcattg
     9241 aggttagcag tggctgggtc cgtaaacttc aaatcaacat agccacgtaa acttggaaac
     9301 atctcagcaa ccggcaaacc agcctcaccc cttgaggtga tagcggttaa tgtggcgtta
     9361 ggatgcacgc tgagcaaacg caatagttct acgccggtat aacccgtacc gccgacaata
     9421 ccaattttaa tttttttatt gttttccatc attttgccaa aaataaacac ccatgcattt
     9481 catcaatctt gattataaag gcttacgcgt aagcttgaaa taaaaatgca cacaaaataa
     9541 aaannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaaataaa aatgcacaca
     9661 aaataaaaaa ggccgcaaaa gcgacctttt ttaaacctat ccgccttaaa ttagcgttta
     9721 gagaattgtt tacgacgacg cgctttgcgt aagcccactt ttttacgctc aacttcacgt
     9781 gcatcgcgcg ttacaaaacc cgcatgcgat aacgcgcttt tcaagttagc atcaaaatca
     9841 atcaacgcac gggtaatacc atgacgcact gcacctgctt gaccagactc gccgccacca
     9901 atcacgttta ccatgatgtc aaagcttgct aagttgttgg tgagttctaa tggctgacgc
     9961 aaaatcatgc gtgatgtcac gcgtgagaag tattcgtcca ctggcttatc gtttacaacg
    10021 atattgcctt tacctgattt taagaacacg cgcgctactg aacttttgcg gcggcctgta
    10081 ccgtaattgt atttaccgat catcttattt atccttagat tttcaatgct tgcggttgtt
    10141 gtgccgcgtg ttcatgcttg tcgccagcat aaactttcat ttttttaatc attgcgtaac
    10201 ctaatggacc tttaggtagc atacctttaa ccgctttttc taatgcgcga ccaggaaact
    10261 tgtcttgcat tttagagaaa gtcgttgtgc taataccacc tgggtagcct gagtggctat
    10321 gataaagctt accgtcagcc ttattcccgg taactttcat tttgtcggca ttaatcacga
    10381 caatataatc acccgtatca acgtgaggtg tataaatcgt tttatgttta ccgcgtaaac
    10441 ggtgtgcaac cgcgctagct aaacggccta gcactaagtc tgtggcatct accacaaacc
    10501 aatcgcgctt cacttcgtgt gactttgctg agaaggtttt cattgtctta tcaatactat
    10561 gagctaaaaa aattaagagg cggattttat gctgaatcac acttgtttgt caaacactat
    10621 gttgtgcttt tattatctcg ctacaggtaa atgatgatac tacatgaaat aatgggtgaa
    10681 ataatgggca accaagccca caaacacggc aaaaccaaac caattgttat gcaaaaacgc
    10741 tttgaagcac tgtgattttt cgcgcgcttt aattaagcgg taatgataga ttgccactac
    10801 tgcagctacg ccaagcccgc cataaaatcc atagcccagt gccagttgct ggcctgcaat
    10861 cacgagcagt aataaagttg ccgcatagca caacataacc gccagtacat caaaccgacc
    10921 aaacgtaatc gccgaagaca taataccgat ttttaaatca tcgtcccgat ccaccatggc
    10981 gtattcagta tcgtatgcaa ttgcccaaaa aatatttgct gccattaaaa gccagacaac
    11041 cgttggcaca ttgccagtag tcgcagcaaa ggccatggga atacccatac caaatgcaat
    11101 gcccaaatag gcttgcggaa tcgccaaaaa tcgtttggta agcgggtagg tcgcggcaaa
    11161 aaacaatgct gcaaatgaaa tataaattgt tagcctgttc atcaaacaca ctaaaccaaa
    11221 ggccgctaaa cacaagcctg cagcaagcca gtaggcctct tttttgctca cttcattggt
    11281 aatcagcggg cgatgtttgg tgcgctcaac caagccatca aatgggtggt cggctaggtc
    11341 attcattacg caaccagcgc tacgcattag caccgtgcct gtgataaaaa tcagcacgat
    11401 aatccaatcg ggcttgccct gcgcagcaat cagcaacgcc catagcgtag gccacaacag
    11461 caataaaatg ccaataggct tatctagtcg catcaaacgc tcgtaggcat taattttttt
    11521 gttaagttgt tgtagattca ttggtctaat aactgcggca aaaatacttc tgtcactaaa
    11581 atattagcgc aatttagatg aaatatcgaa cgccgcgccc ataaataggc aggcttatca
    11641 gcgagatgag acgccgccaa tcgataaagt gcgtgattgg gtaataattt tttatagctt
    11701 aacggcgtgc gtttcacttt tggattggca aataaagttg cgcctaacgg cttgttgcct
    11761 agcttaccta gcccatacca tgcaccgcgc aagctggctt gcggcaatac actatgcgca
    11821 aataccaccg gctgctgatt gcctaacagc aacacctcgc gcactaaagc gcgcttatgg
    11881 ggctttgatt gcaacaaacc tgcctcatca tccagcggtt ttgcgtcctc taaacgcacg
    11941 gtttgtaccg caaaattggc atagcgccgc tgcaaacgcg ctgtgagaga gcctgtttcg
    12001 atcaaccaag aaacaaacgc gccacttgca aaaggtttgc gtagccaacg gctacgttca
    12061 ctggaataat gttgctgaat tttcacagcc gaattatcgc ataagtcttc gtgcgctgaa
    12121 tgtcgcgaac atcacggtag tcatttcact gttatcgata gggctgtata ctcatcatgc
    12181 tggtcagcaa aacaggtcaa cttttatgat aaataaagtg ctttttatgc caattttcac
    12241 atacaacctt ttttgatgcc ctgagagcct ttatttataa ggctctcagg gcattgtttt
    12301 ttgctcgggc gttttgaagt gaaattacat tcgatgattg ctattttatt tctgcaaaat
    12361 tatctttatt ttttagctaa actttcacta aatctgccgc acttgccaac catctctcca
    12421 aatgtttttc aaccgctgct ggatattgtt gaattaaatc atccgcacaa tttttggcag
    12481 cctgcaacaa atccgcatcg cggttcaaat ccgcaatttt gagcatcggc acaccacttt
    12541 ggcgtgtacc taaatattct cctgggccac gcaaatttaa atcagcttgg gcaatggcaa
    12601 aaccgtcatt actctcgtaa atcactttta gtctcgcccg tgcggtttca gatagtttat
    12661 tttggtaaag caaaatacaa gtgcttttag tagcgccccg ccccacgcga ccccgcaatt
    12721 gatgcagttg gctcaacccc atgcgttcag cgtgttctat gaccattaaa ctggcattgg
    12781 gaacgtccac gcccacttct atcaccgtgg tggccactaa taattgcaaa gtgtttgcac
    12841 taaactgcgc cattaccgct tgtttttccg cgggtttcat gcggccgtgg attaaaccga
    12901 tttttaattc aggaaattca ctttgcatta acgcgtaagt gtcgttagcg gtggcgagct
    12961 gcaaggcttc tgattcttca attagcgggc aaacccaata ggcttggcta cctgccaagc
    13021 aagcttcacg cacgcgttgc aaaatttctt cgcgacgctc atctgaaact aatttcgtca
    13081 caatgggcgt gcggccgggc ggcaattcat caatcactga aacatctaaa tcggcgtaat
    13141 agctcatcga aagcgtacgt ggaatgggtg tggcggacat catgagctgg tgcggctctg
    13201 gttcgccttt tttacgcaag gctagccgtt gttgcacacc aaagcggtgt tgctcatcaa
    13261 taatggctaa gcctagcttt ttaaactgca cggcttcttg aaacaacgcg tgtgtgccaa
    13321 cgaccatctg cgcatagcct tgagcaatgg cttgcaaagc tgcctcacgc tcttttttag
    13381 tttggctgcc cgtcagccac gcaatgctga tatttagcgg tgtaagccag cctaacattt
    13441 tttgataatg ctgttcggcc aaaatttcag tgggcgccat cagcgccact tgccaaccat
    13501 tttcaatcgc ttgcagtgcg gccatgcaag ccacaatggt tttgccactg cccacatcgc
    13561 cttgcaacag gcgttgcatg ggatgatgtt gtgtcaaatc ccgctcaatt tctaccgcta
    13621 ctttttgctg tgcctgcgtg agcgcaaacg gcaaactttt gagcaaactg gaaactaatt
    13681 ttttagaagg cgcaaaagcc ggcgcattca cgctacgacg gcgcgcataa tgtttgcgca
    13741 tggaaagttg ttgggcgagc agctcatcaa atgctaaccg ttgccattct agggtggttt
    13801 tattctctaa ggcttgcaag tcagcatcgg gcggcgggtg atgcaacgct tttaaactgg
    13861 ccgcaaaact tggtaaattg agcttggcat atacactagc aggtaatgtt tcgtgcaagc
    13921 cattgtgcac gagtgcaatt gatatagcct tgcgcaagct cgcctgcgtt aaacctgcta
    13981 cagtggggta aacaggcgtt aaggtttctg ccacctcggt tttttcaccc acctttttgc
    14041 aagtgggatg caccatttca tagccaaaat aaccatgacg tacttcaccg taaacacgga
    14101 ggtggcttcc ctcttttagc attgcaattt ggctggggta aaaatgtaaa aaccgcaatg
    14161 taagttgtcc gcttgcgtct tctaatcgcg ctatcaaggc ttttcgcggc ttgtaagtga
    14221 cttccatatg cacaatttca ccctcaacca acgcggcctc gcctagccgt aaatctctta
    14281 ctactgtgat atgcgtttca tcgatataac gtagcggtaa atgcaatagc aatgcttgca
    14341 actgactaat gccaagctta tttaaacttg cgagcaacgg tttgctaaac gttttgaggg
    14401 ttgaaagtgc tgtcatatca acagtaaaag ctcggttaat gcattactgt gtgcgggtaa
    14461 actggcgatt attgtgaaac ctgctgtgtg ccatttgccg ttgagttgcc tttggcgcgg
    14521 ttgatgcgga ctacgttggg gactttgcgt aacgcacgca tgagctcagc aagatgcaca
    14581 cgatcacgca cttgcacggt aaaattaagc cccgtataca cgctgttgtc agattcctcc
    14641 atgctcacat tgtcaatatt agagccagcc tctgcgataa tggcggcaat catcgccagc
    14701 atgccgggtt tattttctgc caccagtttg aggtttactt taaacaaacg tttgctatct
    14761 gaatcccatt caacatccag ccatttttct gggtccaagc taaattttct gatggctggg
    14821 caatcatgcg tatgaatgat taaaccttta tctttgttga taaaaccaag aatcgggtca
    14881 cctgggatgg gtcggcagca cgaggcaaat tgcacactca tgccctcaga cccgcgaatg
    14941 gtaatggtgt taagcggctt tttgttggcc ttgccaaata atttgccaaa gagtttgctc
    15001 accacgccca ctgtttcatc cgtaggctgc ttgtcatcga tagccaataa ttgatgtgcc
    15061 accataacac tctgccgctt gcctaagcca atgtcggtca gaatgtcttg tttatttttt
    15121 acactgtagt cacgaatgag cttttcccat tgtttttctt ttacggcaga aggctccaca
    15181 tgcatggcgc gcaaggcttg attcaacata cgctcaccaa tgtgtgtcga ttcttctgac
    15241 tgcatggttt taagataatg ccgaatatgc gaacgcgctt tgcccgtttt cacgtaattc
    15301 aaccaagcgg cattcggttt agataatgcc gccgtgataa tttccacatg gtcaccattt
    15361 tttaactctg tccgtagtgg cgctaattct tgattgattt tgacggccac gcaactattg
    15421 cccacatcgg tatgtactgc ataagcaaaa tctaccgccg ttgagccacg tgggagcgcc
    15481 agaattttgc ctttgggcgt aaacacataa acttcatcgg ggaaaagatc aaccttgata
    15541 tgctctaaga actcaagcga atcaacactt tccgattgga tttccagcag gcgctgtagc
    15601 cactgatgcg tttgctgttg taatgcggtg agttcagcgt cgctggactt atacagccaa
    15661 tgcgccgcaa cgcccgcgtt agccacatta tgcatgtcaa tgctgcgaat ttgcacttct
    15721 atgggcgtgc caaacgggcc aaacaacgta gtatggagcg attgatagcc gttggctttt
    15781 ggaatggcga tatagtcttt gaacttacct ggaattggct taaaaaggcc gtgcaacgca
    15841 cccagtgtta aataacaagt gggtaaatct ttcaccacta cgcgaaaacc gtaaatatca
    15901 taaatttgcg aaaatgaatt ggttttaccc gccatctttt tgtagatgct ataaaggtgt
    15961 ttttctcggc cggtcacctc ggcttctatg ccggcttcct tgagtttact ttgtatgctt
    16021 tcaagaattt tgctaatcac ttctttgcga ttaccgcgcg cagctttaat cacctgcgtc
    16081 agcacgcggt agcgcatggg gtagagcgct ttaaagctta aatcttcaag ctcttgataa
    16141 attaaattta aacccaggcg gttagcaatg ggcgcataaa tttctaaggt ttctttggca
    16201 atacgcttgc gcgattcggg cttcatggcg gtgagcgttt gcatgttgtg cagtctgtca
    16261 gccaatttaa ctaaaatcac ccgcacgtcc tggctcattg ccagcaacat ttttctaaag
    16321 ttttctgcct gtgcttggct agcactttga aattcgattt tatcgagctt ggtcacacca
    16381 tcgaccaatt cagcgacttg tgcgccaaat ttgtctttaa tttcttgggc agtaatggct
    16441 gtatcttcca ccacatcgtg caataacgct gcaagaatgg tcggcacatc catgtgcaaa
    16501 tcggccaaaa tacaagccac agacacagga tgcgtaatat agggttcgcc cgttttacgc
    16561 accacgccat catgcgcgac gaaggcaaag cgataagcct cgcggactaa agtaacgtcc
    16621 tgaggcttaa gatattgctt aaggagtatg gtaagtgctg aatcttcact gtctgctggc
    16681 agcaaagggg cttgcgcaag tatcgctaaa gctgaatttt ctaaatcagc ttttgatgta
    16741 tccgcctgag aatgttttgc ggttttgggc atcgcatgtt caccgcgcgc tgcaatttag
    16801 cgtggctgta aaatctcgac gccaacttta ccagcggcga tttcacgtaa cgctaatacc
    16861 gtggttttat cgcgcaagcc ttgtgtgttg atgagcggct cagcgccatt atgcaattga
    16921 cgggcacggt aagcggccac taacgttaat tgaaaacggt ttggaatttg gtctaaacaa
    16981 tcatctactg taatacgtgc catggtgttt cctattgaat aaagtaaaaa tcttgaagaa
    17041 gctaagtcag agattgaatc aaacttgcat agcgctgtag ctgtgccatg cctcttagcc
    17101 gttgcgcgcg aatgattgcg ctaatgtctt gaagtgctac atcaaaatca tcattgattg
    17161 taacataatc aaacgcactt acatgccgca tttcctcgcg ggcggcggcc acgcgtttag
    17221 caatgacttc tgcactgtct tgcgcgcgcc cagtcagcct gcgttctagc gcctcaatcg
    17281 aaggtggcaa aacaaaaata ctggttgctt gtggaaacaa acggcgaact tgctcagcgc
    17341 cctgccaatc aatttccaat accaaatcaa agcccgcttt tagtggccct tccaccgcag
    17401 cttgcgatgt gccgtaacgg gcatcgtgta cttgcgcgct ctctaaaaac cgcgcttcgc
    17461 ccagcatgct taaaaatgtt gcgtcatcta caaaatgata atccaccccg tctatttcac
    17521 tggctctagg cttgcgtgtg gtgtgcgaga ttgaaagctt taattgtgaa tcttttgcta
    17581 acaacgcttt tatcaggctg gttttacccg caccagaagc cgctgttatg ataaataaat
    17641 tgcctgttgt catcgtaaat tcttttgcac ttttgtattt taaaatcata ccatttttac
    17701 tgggtatttt gcgatgcgca aaacttctga cgcatcacaa tattcggcac tcaaaaacga
    17761 tgttttagtg gcaaaactct cccgagaaaa aatttatgct ctgcgagcct tataaacaaa
    17821 ggcttccaga gcaacgaaaa aggtttgtgt ttaaaacctt cattatttaa tgtgaattta
    17881 cggttgtaat cggtgtaatt tcttcatttt ttatgtacgt gtaattgcat gtgaatcacc
    17941 tgctaacatg gtaataaacc ccataacacc cgcttaatca agcaattgaa tgttgccatg
    18001 gtcggtatga tggcgtttaa tatgggtaat cacacaatat acaaacaaaa ttgaccgcaa
    18061 acgcaaccgt taagcatatg gataaaaaat taacgcccct agaactggct cgccagacgt
    18121 tgattcagct ttctaaaagc cagagtccgc ccacgcctga aaactaccgc cgtgtgtatg
    18181 atgaaattgc aggcatcaaa tctgttgata gcgtagacgt gcttggtaaa tctttggaaa
    18241 aggtattaac ggaacttggt aaagacaggc ctaagtatct cgccacttcg caaaaaattg
    18301 ccgcatttat taaaaagcgt gatgctgaga accttgaaga ccaactgcgt aaactcatgc
    18361 ccaccagcgc caatgaagcc gatggcgtca attggcccac gcttttgcgc tatttgctga
    18421 agcagctcga tgttaaccac acaggcacta ccttaagccg aaaaaaagag gggctcaacc
    18481 gcgtcattat caatttcgcc aatgacccga gccaattagg tgagaaaatt caggcgctca
    18541 tcacctcttg gggagatggc cagccgtcag catctaccaa cagtgcaaca gcactccaag
    18601 ctgaatcaac actaatcgtt gaggtagcct ctgcaaccca aacaaatgca gcgctaacag
    18661 agagtgagca agcactcact acagcttggc gagatttact cacaagagca atcaagctgg
    18721 ttgtgcttcc gcagtttgca gacatcccgg ccgcctctga caaaatcgag aatgtcattc
    18781 aactcgcgca aaaagccaac accgttgaag aaatcaatgc actgggtgaa acgttaaaat
    18841 cattcatatt gcgcgctgaa atgcgtaacg atgcgcagca ccgcatgcaa gagtcattgg
    18901 ttcaaatgtt gcgtttgctt atttctagca tgggtgaact cacaattgaa gacaaatggc
    18961 tgcatggtca aatttccatt gtgcaagata ttatttcaaa accgctggat attgacgccg
    19021 tttataatgc tgaaagtagt ctaaaagaac tgatttataa gcaatcaaat attaagcctg
    19081 gccttattgc cgcaaaagag acgctcaaag gcatgatgag cagctttgtc agcgggttag
    19141 ccgatatttc aagaagcaca gggacttatc aagccaaaat caatcaatac caagtgcaaa
    19201 tttcagaaac agaagacatc acgcaactta atacaatatt gcaaaatttg gtgggtgata
    19261 ttcgcgaaat gaatgccgat gcacaaaatt cacacaatgc ctttgaggaa acacagaaaa
    19321 aagttgaaga agctgaaaag caaatcaatg aactgtctgc caagctggat tacatcagcg
    19381 aagttgcaca ccaagacttt ttaaccggtg cactcaatcg acgtggcatg gatgaagcaa
    19441 tagatcgaga atttgaacgc gccgaccgtc acaacacgcc catctcactc gccatgatcg
    19501 atattgatca cttcaaaaaa atcaacgata ccatgggtca ctctactggc gacacggcgc
    19561 tcgcgcattt ggccaaagtg gttaaaagca ttcttcgtag caccgatgtg ctggcgcgtt
    19621 atggtggcga agagtttgtc atcttactgc caggctcaaa acaaaatgat gccgtgaatg
    19681 tagttgcagg gatacaacgc gacctcacca aaaacttctt tcttcacaac aatgaacgcg
    19741 tacttattac gtttagtgcc ggcgttgcag aaagagtcac tggtgaaaat attgattctg
    19801 tcttgccaag agcggatgca gcactctaca ttgccaaaca atctggcaga aaccgggtgg
    19861 ttggtgcaga accgctaaat aattaacggc aacgttacgc ttatttttct attcaatatt
    19921 ttgaacctgc tcacgcatct gttcaattag cacctttagt tccatcgaca cttgcgaggt
    19981 ttgcgtagac acggactttg accccagcgt attggcttca cggttgagct cttgcattaa
    20041 aaaatcgagc cgcttaccag caggtgcatc cccgttcaaa atacgtttta cttctgatac
    20101 atgcgcggtt agccgactga gttcttcgtc aacatctacg cgttgcgtaa acaacaccaa
    20161 ctcttgcgct atgcgttcag gatcaatgct ttttaatgtc tcctgcaatt tattttctag
    20221 tttggcttgg tattcttttg caagtgtagg caataagggt tttacctttt caaccaacgc
    20281 ttccatctga tggatgcggt ctaaaatcaa ctttttaagt ttatcgccct cgcgcgcgcg
    20341 cgaggctttc aaatcttgta acccctgctg cactaattgt ttgatgtctt ccagcaataa
    20401 gtcttggtca atgccttgcg taagcacgac ccccggccag cgtagaatat ccgccatgct
    20461 taactcacgg cttgttggaa agtaggcctt cgcttgcgat tgcatcaccg ccaattgctg
    20521 catcaatgca ccatcgagtg cggcttcctg acttaattgt gtccgctgaa ttaagttcac
    20581 cttgcactct attttgcctc gatttaattg tgcgctaatt aactcgcgta ttgtcggttc
    20641 aaaccctctt aaattttcat ctaatttaaa atgtaaatct aaataacggc tatttaccgc
    20701 ccgcagctct agtagcaagg tcgcattgac gattggtttt tcaagggcgg caaatcccgt
    20761 catgctataa gtcatttgta aattcctaaa aaattaaaat aaaatttgca tcatggtatc
    20821 aaataccaat gatttcatgc caaaataatg ataaataact ttaattacaa tcaattacgc
    20881 gctatattgc cgaatatact ctaagttata tggcaaaaat tacctaaact tgcgttaata
    20941 tatttttaat aaaccctcat atttttgaaa aaacgaaaac tctgtgtctt ctactaaaaa
    21001 cttagcctta cctagcggct accagctaca agattataaa attgataaag tcttaagctc
    21061 tgggggcttt agctttgtgt atttggcgca tgataaagac aacaagcttg ttgccattaa
    21121 agaatatttg ccaacttcga ttgcattgcg tagcaatggc gcaactgtta tgcccaaccc
    21181 agatgacgca gccttattta gacatggcct gaaatgcttt tttgatgaag gcttgtcgct
    21241 tgctaaaatt gatcatcgaa atatcgttcg cgtcattaat ttttttcgtg ctaacgaaac
    21301 tgtgtatatg gtcatgcagt atgagcaagg taaatcatta caagattaca tcttagggca
    21361 aacagaatta gtttcagaga tttttattcg gcaagtattt agtgagctag caaaaggttt
    21421 gcgagaagtg catgcgcaaa aattattaca tttagatatt aagccagcca atatctatat
    21481 taggttagat gaatcgcctg tattgcttga ttttggttct gccagacaag cgctcaatga
    21541 aaaccttgcc aaatcgtcac ctagttatac cccagggttt gcaccgccag agcagtattt
    21601 cgaccgtaac ttattagggc catggagcga tatctacagc atcggcgcta cgatgtattc
    21661 ttgtttaacc cgcacctcgc ctgcggcggc taatttacgt gtaaaaaatg acttgcttgt
    21721 tcctgctgta aaattagggt tatcaaatta ctcgcaaagc cttctcgaaa ttattgatca
    21781 atgcctaagc ctagattatt tagcgaggcc gcaaagcgtg ttttctttgc agaaatcatt
    21841 acttaaatcc atgccctcac ctaagaaaaa aagtcggctt gataaaattg taggcgtcct
    21901 aaaaagccca gtatccgacg tatttaacac gcctattatc agcaaaaaat ctcaatgaaa
    21961 tttactattt ttcaaaatag cctgcaaggg cctcgccaat acaatcaaga cagattggca
    22021 tattcctata gcaaagatgc attgctactg gtggtggctg atggcatggg tgggcatcgg
    22081 catggtgaaa tggccgcgca attagccgtc accaccatga ccgaagcctt tcagcgtttg
    22141 gccgtgccta ccctgactag cccggctaag tttttaattg atcatattca acaaattcac
    22201 gacatgattg atcagctcac tcaagaacgt gagatgctcg agtcacctcg cacgacaatc
    22261 gtggcagcca ttgtccagcg tggcatgctt tattgcgcgc atgttggcga ctcgcgactg
    22321 taccattttc ggaacaatca tttactctat cgtacagaag atcactcagt ggttcagtcg
    22381 ctctataata agggaattat taataaaagc gagatgacca cccatcctta tcgccataaa
    22441 gtatacagtt gcctaggtgg cgatacaccc cctaaaatcg acctgtcgga tcgtcaagaa
    22501 ttggcggaag gtgataccat tttactttgc acagatggcg tttggggtgc attaacagat
    22561 gatgaaatca aagtcatgct ctatggccca tccattactg ataacgtcac cgcccttctg
    22621 aataaagcag aattggttag ccaagcgcaa ggtgacaata tgagtgccat tggcctacag
    22681 tggggcgata aactcagtga cccttttgca gtatcaacca ttaccatggc aatgggtgcc
    22741 accaccacta tcatgaaccc tgtcaaacgc aatattttag gcagtgacga tacctttact
    22801 gacgattttt cagatgatga tattgaaaat actattaatg aaatacaacg ggcgcttcaa
    22861 aaaaacaagt cagccccata aaaagcatca tcatcaaact tggccacact taagccaccc
    22921 gcttttataa aaaaattatc aggattaaac catgcaggcc aatacaagca atttagaaaa
    22981 tcttagacca agtgctcgtg ctaacaatca gttacgcgat attgaaatca ttcgcaaata
    23041 tactaaacat gctgagggct cagtcttagt taaatttggg gacacgcacg tgctgtgtac
    23101 agcaagtgta gacgaaaaag tgccggggtt tttaaaaggc aaggggcaag gctgggtgac
    23161 cgcagaatat ggcatgcttc cccgctcaac aggtagccgc atggacagag aagcgtcaaa
    23221 aggtaagcaa tcaggccgta cgcaggaaat tcagcgttta attggccgct cgttgcgcgc
    23281 cattattgat ttagaaaaat taggcgaacg cagcattcaa attgactgtg atgtcattca
    23341 ggcggatggc ggtacaagaa ctgcaagtat cactggcgct tatgtagctt tggcagatgc
    23401 aataaaccac ctacttaaaa taggcaaact gcaaaatagc ccacttaaac aagcggtagc
    23461 cgctatatca gtaggcgttt acaaaggtca accggtactt gatttggatt atattgaaga
    23521 ctctgattgt gatacggata tgaatgtggt catgactgct gatggtggtt ttgtagaaat
    23581 acaaggaacc gctgaaggcg agccttttga ccgtgccgcg atgaatgcca tgctcgattt
    23641 agctgcacac ggtattgggc aactcgtgat taagcaacaa gaggcattaa atgtttaaaa
    23701 aactggttat cgccacgggc aatcagggta agctacgcga aattcggcat attcttggcg
    23761 aacttgatat agaaattgtt ccacagtctg cgcttaacgt gcctgagtgt gaagaacctt
    23821 tctgcacctt tatagaaaat gcgcttacca aagcacgtca tgccagtaag cacactggct
    23881 tacccgcatt agctgatgac tctggtttat gtgtggacgc cctgcttggg gcgccaggtg
    23941 ttctttctgc taggtatgca ggggaaccga aatcggacga acgtaataac caaaaactgc
    24001 tcgaagtatt gggtagtgaa aacaatcggc atgcacactt ttactgcgtc atggttttgg
    24061 tgcgccatga acatgacccc gagcctatca ttgctgaagg tcaatggcat ggtgaaattc
    24121 tcaatgaata tcgtggcaca gatggttttg gctatgaccc gttatttctg gacgctaaaa
    24181 ctggcaaaac agttgccgaa ctaccgctcg aaataaaaag ccgcatcagc caccgcggtc
    24241 atgccatggc aaaattgtta caaaagcttg agaggttaag tcatgactaa gccaagtcac
    24301 actgtttggc caaagtggct acgcgatgat ggcagcgttg tttcgtgtac cgaaaaagtt
    24361 aaagtaatga cagaaaactt tgacgaaatt aagcaaatca cccaagatgc gcttgaagat
    24421 ggtttgttaa tggaggtttc tgagcaacaa atgcgcgaag cactgcatca actcgttgac
    24481 tcactaatca atccctacaa aaggtaatct actataaaaa agtttattga aatcaaagcg
    24541 gataacacgt ttaatcaaaa gcctcatcct ggcatcgttc cactattagg caatcataca
    24601 atttcagggc taactgattc accaccgcta tcgctgtaca ttcatattcc ttggtgtgtt
    24661 caaaagtgcc cctattgtga ttttaattcc catgaaagtc gtcatcaaca cggcgagatt
    24721 cctgaaagca catatgtgga tgcgctcatt gcagatttag aacaggccac acctaaagtc
    24781 tggggcagga aaattaagag cgtatttttt ggcggtggta cacccagcct atttaagcca
    24841 gcatctatcg atagaatatt aagtcatgtt cgcgcactca caccactaga ctatgatgcc
    24901 gaaattacac tagaagcaaa tcctggcacc gtagatatcg ctaattttat tggctacaaa
    24961 caagcaggag tgaatcggtt gtctttggga attcaaagtt ttaatgcgaa acacctcaag
    25021 gctttagggc gtattcatac ggacaggcag gcaattcatg cggcagagtt agcactcact
    25081 acctttgagc gcgtaaattt tgacataatg tatgggctac ctaatcaaag catgacgcat
    25141 gccctagaag atgccaaaca agctatcgcg cttaatccag atcatctatc gttttatcat
    25201 ctgacattag aacccaacac gccgttctac cgctcgcccc ccattctacc tgacgatgat
    25261 acctgcgcgg aaatgcaagt agaaatcgaa aatttattgg cagaacacgg atatgaacac
    25321 tatgaaactt ctgcattttc taaacctggg aaacaagcaa agcataactt gaactactgg
    25381 cagtttggcg attatttagg tattggtgcg ggcgcacata gcaaattgag ctatcacgat
    25441 aaaattacgc gtgaaactcg ccctaagcac cccaaagctt atatggaaca atccctacta
    25501 ggaaatgctg cagataacat ctggaatatt tctgataacg aattagggtt cgagtttatg
    25561 atgaacgcgc tacgactaac agatggcgtg cctgtaaacc tttttcaaat gcgtactggc
    25621 ttacacatca atacattaga aggcagcata actcaggcac aaaataaaaa attagttgtg
    25681 gtgaaaaatg actacataaa acctacgcta ttgggacaac gctttttaaa tgaattactt
    25741 acgttgttta tgacaaatta gaatttgctc gattttttgg cataccttaa tcagcaaact
    25801 ctaatttttc tctcacatca agcatgtctc tcagtaatat tatttcttta aacacttcaa
    25861 aactaaaccc tgaccgttcg ttattacgat tatcaagcaa taattcgtct aagtaacttt
    25921 tggtttggga tattttttgt gcttctttct cacaatttgc ccataattga gtcattttgg
    25981 ttgagatgtg tgcgaaagct tctaagctac tattaattgc tgagggcaac gttgaactgt
    26041 cccaagaagg catcattaca ttaaaatttt cgtgcaggag ttttgcatac tgcataaatt
    26101 cggctttctg attggtttta cgataaatat ccaatagaag aagccaatgc tgtaaggttg
    26161 ccttaggcgc tgcagagatt tgtgctttta gcaattttat tgcctcatcc tcacgttgta
    26221 tcgccaagaa gatttttgct tgttccagtg cttgggcagc gtcatcttca aaatgcggta
    26281 tacctacgtc acttaagtct gtcactatca ttgagccaga atattcactc tttacgacgg
    26341 gttccaacgg aaaatccgaa gcaaaaattg taggtgatcg ctttgttttt aagaagctat
    26401 tatcattaaa taactcagct ttcatattac cataactacg ctttgctctt aaatatacat
    26461 tacgtggatt taagaaattt ttttgttgct gattaaaaaa tgcaagggca agaaataagc
    26521 tcaagacaaa aagtaccgtc agaattggct gtagcaagta ttttgctgct ttaggactag
    26581 cattggttac tgagcctgca acatgatggc caagtgcggc tgattcaatc gatgattctc
    26641 cagcagaagc agttgatggt acctgtgctg actgaatatc cacttgtgct aacaagcgca
    26701 ttaactctat ctttaaactt gcgagtagtt ccacgagcgc tagtaattta gcatttagct
    26761 ttatcaactc ctctttcaaa aaagtatttt taatcaaaag ataatcatat gctttttgct
    26821 gcgttgcgtt tggtgcaagt gcgtcctggc tggcgtaatt ttttgcctgt ttaccaacta
    26881 gattggtacg actatctttg gattttgcac tgcctgttcc taaaaattta atatttggaa
    26941 taataatgac cgtcatttga tctgcgcgat tagatggatt taaatttggt tgaatgtgac
    27001 gacttaattg caatgatttt gaaataaatc gctgttgcat ctgtttactg tttgggtata
    27061 gcagtcttgc aaggtcactt aaactctcac ccgccaagag tgaccaaggc gtagaagtgc
    27121 tctccaaggt atattctgta gcatttgaat aggcatcttc atacaaaaac tcttcagcat
    27181 aagcatagtt tgaaaacact aggctggcta aaaaaataat aatattgata cgaagcgaat
    27241 gggtatttag tttcattgaa ccagattaat cactttaatt ttttgcccaa gcacaaaagc
    27301 tcaagcaaag actatattta taatgtgatt aattgtattg tttacaatga aactatcaat
    27361 gacccgtttg ggcttagact accgtctggc gcggactatg gctgccttca cttgggatgg
    27421 agctgtgccg ccaatatggt tgcgactatt tagtgaccct tcgagcgtta aaaccgaaaa
    27481 gacatcttca tcaatttcag ttgcaaactg ccgtaatgtc tctaacgata aattacttaa
    27541 ttcaacatta tgatcaacgg cataacgcac ggctaaagca accacttcgt gtgcatctct
    27601 aaaaggcatg ccttttttgg tgagataatc cgccaagtcg gtagctgttg caaagccttc
    27661 gctagcagct tggcgcatat tatctttatt gactgtaaag ccagccttag tgactacgcc
    27721 attttcaatc aatggaaggc gtaacatgtc tgcataaatt tccagtgtga ctaataaagt
    27781 atctgctgta tcaaatagcg gctctttatc ttcttgatta tctttgttgt aggccaaagg
    27841 ctggcctttc atcaaagtga gcaaggcagt taggtggccg taaacgcggc cggttttacc
    27901 acgaaccagt tcggggacat ctggattttt cttttgcggc ataatcgatg aacccgtgca
    27961 aaatcgatcg gcaatatcga taaaggcaaa acgcggtgaa ctccacaata tcagctcttc
    28021 tgaaaagcga gataaatgca tcatcaccat cgaagaggca aacgtaaatt caatggcaaa
    28081 atcacggtca gaaacagcat cgagtgagtt ttcacaaact ccatcaaagc ccaataattt
    28141 tgcaacacgt tcacgattaa tagggtagct cgtgccagct aaagctgcgg cgcccaaagg
    28201 taactgattg atgcgcttat aacaatcgtg aaagcgcgca acatcgcgtt taagcatttc
    28261 aacgtacgcc atgagatgat gcccaaacgt aattggctgg gctacctgca aatgcgtaaa
    28321 ccccggcata accgtatcaa agtgtgcttc agctaagtct aataaacttg tttgtaagcg
    28381 cgataagccc tctatgactt gcaaagtaat ttcgcgcaac cacaaacgca catcggttgc
    28441 cacctgatca tttcggcttc tacccgtgtg taagcgctta cctgcgtccc ctattttatc
    28501 tgttaagcgt ttttctatat taaggtgcac atcttctaaa ttcagccgcc agtcaaattt
    28561 acccgcactg atttcggctt taatttctat taaacctttt tcaatagccg tgaaatcttc
    28621 acgcgaaata atgccttgct cgctcagcat ttgggcatgg gcaatcgacc cctgaatatc
    28681 atacagcgcc agacgatagt caaagcccac cgaggccgtg tattttttta ccaattcagc
    28741 cacaggctca ttaaaacgtc ctgaccaact agtatctttt ttattcaatg cgcttaactt
    28801 ttctgtcact ttaactttct cgtttaaaat taatctatta tgataagtat aatgcaaaac
    28861 gtaatcgctt cgatgccact cttattaaaa tcgcatattt gccactcgga tttgacagcg
    28921 tatttagctg gtttgaataa aagcagcagt aaacaattat gtacctgtct gcgaattaag
    28981 catcatcaca ggttatattc agtaccatga gaaaatcaag ccgcctacca aatttacaca
    29041 atttaggcgt aaatttacgg atactcatca ttgtcaatgc gattattgcc ctgaccgcca
    29101 tcgttctaag caaccatttt gaggactttt acgtagtcat tactacaatt tcatccatcg
    29161 cacagccatt attgttactt tgccttttag tgctgtatgg cagctattcg ttacttacaa
    29221 aactgcgtta ttggcagggc ctgttaggca tttttaccat cgtattactt gtatgtagcg
    29281 gtttttatga tttaatttct catctgctga tttatagtaa cttaccgtct cgtcttaggt
    29341 tgctattttt tgcttccatc ataacggcga tttgtcttta ctatttttat ctgcatcagc
    29401 acgcttattc acccgcggtt attgaggcaa gattacaggc cttacaagcg cgtatcagac
    29461 cgcatttttt gtttaattgc attaacgcgg tattgtcgct gattcgcacg cagcccaagc
    29521 aggcggaaac tgcgctagaa gatatggccg atttgtttcg cgtgttaatg gcagataacc
    29581 gcgatcttgt gcctttgggg caagaaatag ccctatgccg acaatatttg gcgctggaaa
    29641 aactgcgtct tgatgagcga ctgacggtgc aatggaagat tgacgatatg ccaccagatg
    29701 ccatgattcc acctttaata ttgcaaccgc tcatagaaaa tgcggtttac catggcatcg
    29761 aaccgctaga aaaaggcggc accatcacca ttcaaattta cacaagccgc aaagagattc
    29821 acttggtatt gagtaaccct tacaatgaag caagcttgcg ccacattggt aacaaaatgg
    29881 cactaaaaaa tattaaagag cgcctcactt tgcattttga tttagaagcc tctttgaaga
    29941 gctcgcaatc aaatggcgaa tatcgtgtac aaattacatt accgtacaaa gaggtatgac
    30001 cacgatgata aaactgttaa ttgccgatga cgaagcgcca gcccgcaaac gcctgcgtga
    30061 tttactcagc gacatcaatg aagtaacgat tgtggccgac gctaaaaatg gccaggaggc
    30121 gcttaagata gccaatgaaa atctacccga tatcgtttta ttagatattc ggatgccaat
    30181 tatggatggt attgaaaccg cgcagcattt gcaaaaactg gctaagccgc cggcagtcat
    30241 cttcactacc gcttatgaca catatgcttt gcaagctttt gacctcaatg ctgtcgatta
    30301 cttattaaaa cccattcgct ttgagcgcct gcaaactgcc attcagaagg cgcgagcgtt
    30361 attacccaaa caacttgagg cattggttcc gctaagcccg caacgtaccc atttaagcat
    30421 cagcgagcgt ggtcgcgttt tacttatccc cgtggctgat gttatttatt ttcgtgcaga
    30481 attaaaatat atcacggtca aaaccgctga aaaagaatac ttgcttgaag aatcactcaa
    30541 tcatctggag caggaatttg gcaaaatttt tattcggcta catcgaaatt gcttagtcgc
    30601 ccaagcttat attttaggct atgaaaaacg ccaattaagc catgatgctg agggccttct
    30661 cagcagtgaa aaacaatggg ttgcgctact caaaaatatt ccagatacgg tgacggtaag
    30721 ccgccgccag cagcatctta ttcgtagtaa ttaacgacta aggcttgtgt taaaatgttg
    30781 cattatcgct aataaataag ccattctaca tgacccctaa taccaaccta gctcccgata
    30841 agctcgtgat tgcctctcgt gaaagcccgc ttgccatgtg gcaggcacta catattcaaa
    30901 gtcgattaca ggcactgtac cctgaaaccg ttgttgaaat tttaggcatg accacgaccg
    30961 gcgatcaaat tttagatacg ccacttgcgc gagtgggggg caaaggcctt tttgtaaaag
    31021 agttagaaac cgcgctggca gatggacgtg cagatttagc cgttcatagc atgaaagatg
    31081 tgccgatgaa tctgcccgaa ggctttttga tggccgccac gggcgagcgt gaagatccgc
    31141 gtgatgcctt tgtttcgaac aattttgcca gccttgaagc attacctgca ggtagtattg
    31201 ttggcacatc gagcttgcgt cggcaaagcc aattgcaggc ccgctttcca gacttaaaaa
    31261 ttgaatccct gcgcggcaat ttgcaaacac gtttgcgtaa actcgatgaa ggccaatatg
    31321 cagccattat tttagccgct gctgggctta ttcgcctagg cttacaagca cgtattcgca
    31381 gcgtgatttc gcctgaactc agcattcctg ccgttggcca aggtgctttg ggcatcgaaa
    31441 tcagtgcggc gagaacggat ttattagccg ttcttgcgcc gctcaatcat gctgatacag
    31501 aagtgtgcgt aaaagcggag cgtgccatga gtcgcgcttt ggcaggtagt tgtaccgtac
    31561 cgctgggtgc ttacgccact tgccatgcag atgccattga aatcacaggc tttgtggcta
    31621 gtgtggatgg caaacaaatg ttggtcgaaa ctgctacggg ccaccgcaac aatgcagaag
    31681 cgctaggcca acagttggcc aatcaattgt tagctagcgg cgcgggtgaa attctagccg
    31741 ctctagatgg ccacaaataa ccctgcctat tttcaaaaaa atgactaacc aaacattagc
    31801 gggtttgcat gtggtcgtca ccagacccat tggtcaggca gaaagtcttt gtgaaacaat
    31861 tcgacacctt ggtggatttg catcggcttt ccctttactc gcgatttcac cgttagatga
    31921 ttatcgcgaa tttgatcagg caattgcggc acttgaacat gctgattggg caatttttat
    31981 tagcaccaac gcggtagata gtgccatgca ccgcgtactc gcaaggtatg gcaatgtgcc
    32041 tgaaagcgtt caatttgcgg ccattggcca ccaaacggct aaagaattag cgcgctttgg
    32101 ggtgcatcac gtgcttacgc cacacacccg ttttgacagc gagactttac tcgcactggg
    32161 tgaaatgcag gatgtggcta accaaactgt gattattttt cggggtgtag gtggacgcga
    32221 attgttggct gaaaccctta aagcacgcgg tgccaatgtc atgttcgctg aaagctaccg
    32281 ccgcgtgaat ccgcaaacca acaccgattt acttagtcat ttatggcaac accagaaact
    32341 ggatgcgatt gtggtaacca gcagtgaggc catgcgcaat ttgcttgatt tagcgggcca
    32401 atcagaatgg ctacgccacg tgaccctttg cgtgaatcac gctagaatag cggagttgcc
    32461 gctacagttt gggttaaaag tactggttgc agatgcacca ggagatgacg cgataattca
    32521 acgattggcg caattggtga aacctcatgg gaaatgaaaa tcaagtcagc gttgcagata
//
LOCUS       DAOQSI010000007        31021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_860_length_31021_cov_5.193115, whole genome shotgun sequence.
ACCESSION   DAOQSI010000007 DAOQSI010000000
VERSION     DAOQSI010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 31021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31021
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_860_length_31021_cov_5.193115"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="PKL42_01110"
     CDS             complement(<1..846)
                     /locus_tag="PKL42_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HNU65695.1"
                     /translation="MTTKQHFTRRDFFKLAGAGVALSAVSKVALAALPDIVSAEKANT
                     QAPLHPSTGRHYQPVVTLNGWSLPWRMNNGIKEFHLVAEPVVREIAPGMQAHLWGYNG
                     QSPGPTIEVVEGDRVRIFVTNRLPEVTSVHWHGQRLPNGMDGVSGLTQPAIPPGKTFV
                     YEFEAKRAGTFMYHPHADEMTQLAMGLMGSWVTHPKSPKFMAVDRDFIFLLNAYDIEP
                     GSYKPRINTMLNFNLWTWNSRAFPGIDPLVVRKNDKVRIRIGNLTMTNHPIHLHGHEF
                     EVTGTD"
     gene            complement(849..2213)
                     /locus_tag="PKL42_01115"
     CDS             complement(849..2213)
                     /locus_tag="PKL42_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNU65696.1"
                     /translation="MQYYKFALYSALLSLLLNGCATFSNDGGLADVKALTAPHLKQEV
                     VWPKNEGAKTIVAERVSELLQEPLNADSAVQVALLNNSGLQASLYALGVAEADVVQAG
                     RLPNPKFSMLYARHQGEYKIEQALTFNIFSLLAMPKVLEIERSHFEKTKQAVAIEVLS
                     LAYETRAAYFNAVAANELVRYSVQVKESAEASAVLAQRMVAAGNWNTLEQAREQGFYA
                     DALLEYTSAKLAQNRAHASLARLLGVSAEQLKLEKRLPDIPKTASELQPLDKTAFEQR
                     LDLQAIRLETAALAKQLGLTKTSRFINVLEIGPARVLEGRRGDAYKNGVDIAFELPLF
                     DWGDAKVARAEAIYMQSVHRAAQTAVNAQSEISEAYSHYLATYEMAKYYRDDIVPLRQ
                     KILAENQLRYNGMLVSPFELFADARAQVASVKAYINSLNEFWLAETALNKAFIGKPTS
                     LEGK"
     gene            complement(2312..2686)
                     /locus_tag="PKL42_01120"
     CDS             complement(2312..2686)
                     /locus_tag="PKL42_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65697.1"
                     /translation="MQVFSLKFLRTFILLLLTVFITDTVYASSKMVADMIAGQQDAQN
                     VSEAASHAVHCQNMQAEQAHEKSNSHAGSQHCSHCIACFSMIVQEKLSLKTLQATPSV
                     IPAFVKIYHAPFRAQPQKPPIV"
     gene            2771..3460
                     /gene="aat"
                     /locus_tag="PKL42_01125"
     CDS             2771..3460
                     /gene="aat"
                     /locus_tag="PKL42_01125"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HNU65698.1"
                     /translation="MSYSYYQFPNGRVACIDDDCDFPSLNQALSEPNGLIAIGGDLSL
                     PRLLNAYQHGIFPWFSEGEPILWWSPNPRMVLFPDALKISNSLKKTLKNKPFDVRFNT
                     AFHQVISACSHTPRAGQPGTWITQDIIEAYCTLHNAGYAISAECWQDNTLVGGCYGVK
                     IGKMFYGESMFHLVTDASKVAFVHLVQKLKSEGVGLIDCQMKTAHLARFGAKEISRDD
                     FIDNLSSLTLA"
     gene            3569..4303
                     /locus_tag="PKL42_01130"
     CDS             3569..4303
                     /locus_tag="PKL42_01130"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HNU65699.1"
                     /translation="MTSPNDIPLQKLQFYVTTPYTCGYLPGKLAQSLIAAPQHLVNAS
                     VYNSLIQQGFRRSGNFAYRPHCENCQECVPVRIVLNDFVPTRSQKRAYKQHQHLSTAI
                     LPVDFHEEHYALYHAYQQARHAESLDKAPAQNDVEQYRNFLCQTNVESVLVEFRENGQ
                     LNMVSVIDIVDDGISAVYTFFDATKRASFGTYNVLWQIEWAKRLAHPYLYLGYWVKDS
                     QKMAYKQNFQPQQMYLDGEWRSLKLG"
     gene            4586..5449
                     /locus_tag="PKL42_01135"
     CDS             4586..5449
                     /locus_tag="PKL42_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNU65700.1"
                     /translation="MKKLVIFGVGLIGGSVALALKKAGHATHIVGVGRSLESLQRAVK
                     LGVIDAFETDVRAACENADLILIAAPVAQTTDILKSIQPHLNSNTVITDAGSTKGDVL
                     RCAEKILGPQLHQFVGGHPIAGAEKSGVTAATADLFLGKNVVLTPTDKTNQDALARVK
                     NLWQACGANVSAMPAETHDGIFASVSHLPHLLAFALVDDIAARPNAEQLFSFAASGFR
                     DFTRIAGSHPEMWRDISLANKTALLSEISAFQAELSQVKTLLENCDGAGLQALFERAS
                     AARNAWAKNQQ"
     gene            5462..6757
                     /gene="aroA"
                     /locus_tag="PKL42_01140"
     CDS             5462..6757
                     /gene="aroA"
                     /locus_tag="PKL42_01140"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNU65701.1"
                     /translation="MEQLHLPAYSRAQGTIKLPGSKSISNRTLLLATLANGKTDIHDL
                     LASDDTSRMLEALQTLGVNLENLANNTWLVEGCEGHFPNKGTPEKPVDLFLGNAGTAF
                     RPLTAALAFSNGYYLLHGVPRMHERPIGDLVDALRQAGAQIEYLGNEGFPPLKISPAK
                     LDLSQSIKIRGDVSSQFLTALLMALPLTKQNATIEVVGELISKPYIEITLNLMAKFGV
                     KVERNGWQQFSISNCTEYLSPQVFFVEGDASSASYFLAAGAIAGEVQVNGLGKNSIQG
                     DVQFTEALEKMGAEIHFEENSVIASNVGKLKAITLDCNHIPDAAMTLAILAIFAKGTT
                     TLLKIKSWRVKETNRLKAMATELRKLGVTVKWGDGYIKITPPEKLTPNAVIDTYDDHR
                     MAMCFSLVSLGGVPITINDPKCVNKTFPDYFDAFKKIVS"
     gene            complement(6763..6993)
                     /locus_tag="PKL42_01145"
     CDS             complement(6763..6993)
                     /locus_tag="PKL42_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HNU65702.1"
                     /translation="MTKTFTFAILHFTVAFLVAYLLTGSFVIGGLLALIEPACNTVVF
                     YFHERAWQIFEQKKTASSVTAPFSAMPCAGLH"
     gene            7108..7800
                     /gene="cmk"
                     /locus_tag="PKL42_01150"
     CDS             7108..7800
                     /gene="cmk"
                     /locus_tag="PKL42_01150"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNU65703.1"
                     /translation="MNLPNTDIPVIAIDGPSASGKGTVAQLVAQKLGFHYLDSGALYR
                     IVAYAAQQKDIAWGDAFAVTECAKKLTITFVGEQVLLTTPDFAQKDISNEIRTETIGK
                     GASQVAVHAPLRAALVEMQHAFCKSPGLVADGRDMGTVIFPEAPLKVFLTASTEVRAQ
                     RRYQQLRNKNQPADYESILADLQERDARDKNRAAAPLIMAQDAILLETDHLSISDAVN
                     AVLENFQQKMYE"
     gene            7942..9459
                     /gene="rpsA"
                     /locus_tag="PKL42_01155"
     CDS             7942..9459
                     /gene="rpsA"
                     /locus_tag="PKL42_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNU65704.1"
                     /translation="MASTSKSTTSASSGMESFAALFEESLAHQEMRSGEVITAEVISV
                     DQDHVIVNAGLKSESVIAAEEFKNAQGELDVKVGDFVKVLIEKLEDGFGSTVLSREKA
                     KRMETWLDLEDAMNEGRIIKGFVSGKVKGGLRVSVNGIMAFLPGSLVDVRPVKDTTPF
                     ENKECDLKVIKIDRKRNNIVVSRRAVMEVSAGADREALMGTLAEGAVVKGIVKNITDY
                     GAFVDLGGIDGLLHITDLAWRRVKHPSEVLTVGEEVEAKILKFDQEKNRVSLGIKQLG
                     DDPWVALARRYPVSTRLFGKVSNLTDYGAFVEIEPGIEGLVHVSEMDWTNKNIHPGKL
                     AQLGDEVEVMILEIDEARRRLSLGMKQCQANPWDDFSATHAKGDKVSGQIKSITDFGV
                     FIGLPGGIDGLVHLSDLSWTQSGEEAIRNFKKGDELQAVILAIDVDKERISLGVKQLT
                     ADPSGASSGSDDKADAKPKAKSAKTKEAAKPADDGATAGTTNLGALLKAKLDSKK"
     gene            9504..9785
                     /locus_tag="PKL42_01160"
     CDS             9504..9785
                     /locus_tag="PKL42_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HNU65705.1"
                     /translation="MTKSELITLLAERFPQLVVKDAELSVKAILDAMTDNLATGERIE
                     IRGFGSFSLNYRPPRLGRNPKTGSKVQVPEKHVPHFKAGKELRERVDLG"
     gene            9916..11100
                     /gene="lapB"
                     /locus_tag="PKL42_01165"
     CDS             9916..11100
                     /gene="lapB"
                     /locus_tag="PKL42_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HNU65706.1"
                     /translation="MEFEFWWLLALPLFFSLGWLAGRIDLKQLLSESAALPAAYFKGL
                     NFLITNQQDKAIEAFSEAVQANTDSLELHFALGSLFRKRGEVNRAIHLHLHLLEKKEL
                     EPQQKLAVTAELAQDYLKAGLLDRAEELFESLNDDRYRQPALRALLEIYVREREWERA
                     IKAATELERLSGVPFRVEISHYHCELAVKSMLANDIKTAKFELEQALNANKNCVRANV
                     LLGDLEAEANAHQAAISTWKRIEFQQPEYLGLIAPKLLNSYRALNQTAEGLALLQTYL
                     QTYKLSPLLNVLFEATLAEEGAESAAKLARNELIKKPSLSTLDQLLQARTIVETNQPV
                     QKTKLTSSQDTQLMQQAVRHAIGNKTAYHCEQCGFKAKYHHWQCPACNAWEALPAEPT
                     TI"
     gene            11118..11861
                     /gene="pyrF"
                     /locus_tag="PKL42_01170"
     CDS             11118..11861
                     /gene="pyrF"
                     /locus_tag="PKL42_01170"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNU65707.1"
                     /translation="MTLAHDAKIHDPKIHDPKIIVALDYADATSALKLVEQLDPALCR
                     LKVGKELFTAAGPKLVEQLVQANFGVFLDLKFHDIPNTVAKACTAASQLGVWMLNVHA
                     SGGLEMMQAAQQAVQSSEAKPILIAVTVLTSMNQQTLNQIGIHTDLTTHVQNLAKLTQ
                     QAGLAGVVCSALEASMLRQELGNDFCLVTPGIRPANVNQDDQSRVVTPADALKNGASY
                     LVIGRPITKAINPLKALEAIHAECKLFNA"
     gene            11967..>12454
                     /locus_tag="PKL42_01175"
     CDS             11967..>12454
                     /locus_tag="PKL42_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNU65708.1"
                     /translation="MKKMLLAFIAFLSFSLSAFAAVNINTATQTELETLENVGETRAK
                     AIIEYRNKNGKFKSLDELDKVPGIGEKTLESIKKDGTISGATTVTAPNKNTKNEKPMK
                     EKATKAEKAVATDKPAKAEKASKEAKSASKEDSVKADKAAKDKEKADKAAAKEAEKEA
                     KKA"
     assembly_gap    12455..12554
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12771..14864)
                     /gene="metG"
                     /locus_tag="PKL42_01180"
     CDS             complement(12771..14864)
                     /gene="metG"
                     /locus_tag="PKL42_01180"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNU65709.1"
                     /translation="MSSANQPRKILVTSALPYANGSIHLGHLVEYIQTDIWVRFQKMQ
                     GHTVHYVCADDTHGTPIMLRAEKEGITPEALIASVHQEHSADFKEFLVEFDNYYSTNS
                     DENKVLSQGIYKALKANGKIATKTIEQFFDPVKNMFLPDRFIKGECPKCHAKDQYGDS
                     CEVCGATYNPTELINAYSAVSGAAPVRKETEHYFFKLSECEQFLKDWTRSEAIKGKPT
                     LQGEAANKMGEWFENGLNDWDISRDAPYFGFEIPDAPGKYFYVWLDAPIGYMASFKNL
                     CDKQGLNFDEYWAKDSKTELYHFIGKDILYFHALFWPATLEFSGHRKPTQIFAHGFLT
                     VNGEKMSKSRGTFITARSYLDHIKNPEYLRYYYAAKLNSTMEDIDLNLEDFVARVNSD
                     LVGKYINIASRTAGFINKRFAGQLKPSQNNAIVAEIKAAATVVAEAYAEREYGKALRE
                     IMRLADVANGFVAEKAPWVMAKQAGQDAALQEVCSDALELFRLLTLYLKPVLPKLAAE
                     IEQFLNIAPLTWASVGASLAVNHAINAYEHLITRIDPKAIEAMTEANKENLTATPAPQ
                     PHSEQRHAAHQQNEVKAETVETDYISIDDFTKVDLRIAKIVNAEHVEGAEKLLKLTLD
                     IGEEKPRQVFAGIKSAYDPETLKGRLTVMVANLAPRKMKFGMSEGMVLAASDERGGPY
                     ILSPDTGAQAGMRVK"
     gene            15037..16125
                     /gene="apbC"
                     /locus_tag="PKL42_01185"
     CDS             15037..16125
                     /gene="apbC"
                     /locus_tag="PKL42_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HNU65710.1"
                     /translation="MAISELLVQSTLKVCIDPNTGKDFVSSKSARNIKIDGNDVNVDI
                     VLGYPAKSVMADIQKLVADALLGLAGIGRLTVNVSSKIVAHKAQQGVTLLPKVKNIIA
                     VASGKGGVGKSTTSVNLALALAAEGATVGLLDADIYGPSQPQMLGISGRPESTDGKSM
                     EPMQAHGIQAMSIGFLIDTDTPMVWRGPMVTGALEQLLRDTNWHELDYLVIDLPPGTG
                     DIQLTLAQKIPVTGAIIVTTPQDIALLDARKGLKMFEKVGIPILGIVENMSTHICSKC
                     GHEEHIFGAGGGALMASDYQVDLLGSLPLDMQIRMQADSGQPTVIAQPDSKIAHIYKE
                     IARKAAIKIANASLDHSAKFPNIVIQNT"
     gene            16155..16856
                     /locus_tag="PKL42_01190"
     CDS             16155..16856
                     /locus_tag="PKL42_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNU65711.1"
                     /translation="MAIKKLVSPIQQAFSWVVGGLGILFALRFLPAWLRFAVYGSAVL
                     LLIVYFSLYAMVARHAYDLLAHPPTQKADAALILGNKSYLNGAPNPCLTGRVGAGLAL
                     AQAGLVSTLVMSGGKDNEDGAIEALTMEQYALANGFKGRILLESRSSSTQENFAFSAP
                     IMLNGQIKSVIVVSEPYHMWRAQKLVQAGHLGQDFEVFYAAAASQCWTDWGMLFKGAF
                     REPLAIINNYAKGYF"
     gene            complement(17010..17720)
                     /gene="pgl"
                     /locus_tag="PKL42_01195"
     CDS             complement(17010..17720)
                     /gene="pgl"
                     /locus_tag="PKL42_01195"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNU65712.1"
                     /translation="MNHQTPPQNTRWKSFESIEGLRHAVCDAIVSSAQAAIQQRGQFS
                     IVLAGGHTPRAAYRLLRDLPQEAYLDWSKWHIYHGDERCLPPEHEDRNSLMVEQVWTH
                     FVGIPKENIHDIPAELGPVAGAATYANTLAGAPTFDLVLLGLGEDGHTASLFPNHAVD
                     NSADAVPVFNSPKPPSERITISQNRLNNTRQVIFIVSGAGKQNAVDHWRKGVAIPATL
                     IAPKNGVDVYCYEVKIAP"
     gene            17932..18723
                     /locus_tag="PKL42_01200"
     CDS             17932..18723
                     /locus_tag="PKL42_01200"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNU65713.1"
                     /translation="MLIKDSEVVDLQTPTGEMRAHIFRPAAPGKYPAILMFSEIFQVT
                     APIRRTAAMLAGHGFIVACPEIYHEFEPLGTVLAYDEAGTTRGNELKITKPVDAYDSD
                     ARTVLDYLKSRSDCTGRLGVMGICVGGHLAFRAAMNPDVLATVCFYATDIHKKALGLG
                     KNDNSLVRAGEIKGELLHIWGRQDPHVPLEGRNLVKARLDEVGTRYTWHEFNGQHAFM
                     RDEGHRYDPALARLSWDLLLELFNRRLGWGDLDIENDGSAAESRH"
     gene            18816..20321
                     /gene="zwf"
                     /locus_tag="PKL42_01205"
     CDS             18816..20321
                     /gene="zwf"
                     /locus_tag="PKL42_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNU65714.1"
                     /translation="MAKKIDPCTLVLFGASGNLSRVKLMPGLFRLDAAGRLPEKMAIL
                     SVGRGEVSREAWQADIKGMLDAKFKNGYDQKVFERFIARNHYQANPPTDPDAFKKMKE
                     TLSNEAVFPQNLAYFLSVRPTDFAPVVEQLSGVGLLDESKYWRRVLIEKPFGTDLASA
                     QDLQARLTKHLKESQIYRIDHYLGKTAVQGIMLTRFANAIFEPLWNKDHIDHVQITNN
                     EILGVGDRTTFYNATGALRDMFQSHLLQTLALTAMEKPKDLSPESIRAEKIKLLQSIR
                     PIDASNLNKQAFRAQYAAGRVCVGDGHGENVAGYLDELKRDGIESSNTETYAAVKLWI
                     DNERWKGVPFYVRTAKRMHEGNVAISVKFKKSPMQLVDSQHQNWLVISIQPKETVKLE
                     IESKIPGLDIATRTLSIDAPQRQSGDESIDSYETLMLNLMEGDPSQYLHISEVEAQWK
                     LVDPIVKTWAADNTPLHQYRAGDRDPKESGVIFEAEDQFWRYSIELGGDKH"
     gene            20406..20981
                     /gene="dcd"
                     /locus_tag="PKL42_01210"
     CDS             20406..20981
                     /gene="dcd"
                     /locus_tag="PKL42_01210"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNU65715.1"
                     /translation="MSIKSDKWIRKMAQEHGMIEPFEPNQVKMNAAGERMVSYGTSSY
                     GYDIRCSREFKLFTNLNSTIVDPKNFDPNSFVEVNADYCIIPPNSFALARTVEYFRIP
                     RNVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYANEGCAQVLF
                     FEADADDVCETSYKDRGGKYQGQVGVTLPKI"
     gene            20991..23231
                     /locus_tag="PKL42_01215"
     CDS             20991..23231
                     /locus_tag="PKL42_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/lysine/ornithine decarboxylase"
                     /protein_id="HNU65716.1"
                     /translation="MKFRFPVVIIDEDFRSENSSGLGIRVLAKAIEAEGFEVLGVTSY
                     GDLTSFAQQQSRASAFILSIDDEEIVEEKPEAIEQLRKFVTEIRYRNEEIPIFLHGET
                     RTSRHIPNDVLRELHGFIHMNEDTPEFVARLIIREARTYLDSLPPPFFKALTHYAADG
                     SYSWHCPGHSGGVAFLKSPVGQMFHQFFGENMLRADVCNAVDELGQLLDHTGPVAASE
                     RNAARIYNCDHLYFVTNGTSTSNKIVWNSTVAPGDIVVVDRNCHKSVLHSIIMTGAIP
                     VFLMPTRNHFGIIGPIPKSEFAWENIQKKIARNPFATDKNAKPRVLTITQSTYDGVLY
                     NVEEIKELLDGKIDTLHFDEAWLPHATFHDFYGDYHAIGADRPRCKESMIFSTQSTHK
                     LLAGLSQASQILVQNAEEIQLDRDVFNEAYLMHTSTSPQYSIIASCDVAAAMMEAPGG
                     TVLVEESLMEALDFRRAMRKVDEEWGTDWWFKVWGPTDLSEEGLEERDAWMLKANEAW
                     HGFGNLSEGFNMLDPIKATIITPGLDIHGDFSENNGIPAAIVTKYLAEHGVIVEKTGL
                     YSFFIMFTIGITKGRWNTMVAALQQFKDDYDKNQPLWKVLPEFVQKHPRYERVGLKDL
                     STQIHAVYKANDVARLTTEMYLSDMVPAMKPTDAFAKMAHREIDRVPIDDLEGRVTAV
                     LLTPYPPGIPLLIPGERFNKIIVDYLKFAREFNEKFPGFETDNHGLVKEMIDGKPVYY
                     VDCVAQ"
     gene            complement(23449..23934)
                     /locus_tag="PKL42_01220"
     CDS             complement(23449..23934)
                     /locus_tag="PKL42_01220"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNU65717.1"
                     /translation="MTALSLIVARAKNNVIGLNNTLPWHLPEDLKRFRALTTGHHIIM
                     GRKTYESLGRLLPNRTTVIVTRNPHYHVEGALIAHSLDAAIALCKGDEEPFLIGGAEL
                     YQTGLAKATKLYITEVDLDVIGDAFFPEVDFNHWQLAEQKQHITANGMAYRDLVFIRK
                     N"
     gene            24051..25523
                     /locus_tag="PKL42_01225"
     CDS             24051..25523
                     /locus_tag="PKL42_01225"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNU65718.1"
                     /translation="MQITYPKSFLKLLLIGFALVILPLIAAFVNANLQFNKLTQQSLF
                     NMSQAVETTRASQIMLEELAVMERSARQYFVLRDQVLLSNFNQAHERLGAALNILLLQ
                     PIALPQHTDLMSFTRQENDLFQSITQAQHNKADFDDSMVLNFATLTNLANQISTSNNA
                     LIDQEAVTFKVRVSKVKKALFWQMLTIFPLVLIIAALITWMIARPIRRMDAAINQLGQ
                     GDYEHVIHINGPGDLRQLGSRLDWLRIALKDLHQQKQRFLQQASHELKTPLTAIREAS
                     ELLHDGVAGNLNTQQNQIVTILRDSSLRLQRMIENLLKYTEIQFNAAKSNVSQQALNV
                     VIQKVLQAYALTIAHKNIDVQQSIPDMQVQLDIEKLSTVLDNLISNAVKYTPEHGKIS
                     IHATRTKNDLEIAIKDNGPGIPDANQKDLFSPFYHGEQASDSLVASSGLGLFIAKEAA
                     SALQGELRYITSDAGAHFLVRIPLSSMQPNHEQPTEVTNR"
     gene            25520..26221
                     /locus_tag="PKL42_01230"
     CDS             25520..26221
                     /locus_tag="PKL42_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65719.1"
                     /translation="MKKIQWNSMVVLCIMTIACTSQATKPSNHNHKTQASLQEATPNG
                     IKANEFKSAEVKPVEIAKPVDNKPSLTGLSNTTSRPQVSHYLSFADDFSNFTAEAQKQ
                     VLSNTLQAFAKAPNDIEQRMRLVLIYGLPSSTLLDSAKAQSQLQQILQESVLPSEQQA
                     FANLLFDYIVAVNKASKISREEQKKLETMQQKQEALQTKLENTQQKLEAAQQKIDELK
                     NIEKSMGQREVAPKK"
     gene            26249..27604
                     /locus_tag="PKL42_01235"
     CDS             26249..27604
                     /locus_tag="PKL42_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNU65720.1"
                     /translation="MANPILIVDDDADILKLLEMRLTASGYPVIAASSAQQALTLFGM
                     HPIALVISDLRMPEMDGFALFEAIHRLDSTIPFILLTAHGSIPEAVHATQQGVFSFLT
                     KPFEGKALLEQVAQALRVSPHHPNHQQENDTWRSSIISGSQQMQSLMAQAKLVAESDA
                     SVLIQGESGTGKELLAQSIHHASPRREHPFVAINCAAIPEALLESELFGHKKGAFTGA
                     TRDHQGLFQTADGGTLFLDEIGDMPMSIQAKLLRALQERMIRPVGASTQAPINVRVIC
                     ATHKNLILEMQEHRFREDLYYRINVVSLEIPPLAKRREDIPLLANHFLAVLNKKYHKN
                     INGFAPEAFELLISAPWPGNVRQLQNIMEQVVVLSTTPIITSGLVERALQDNISSIIT
                     FDAARKNFEQQYLINLLKATQGNVTQAARIADRNRTEFYKILDRHHINPALFKTQVTD
                     E"
     gene            27835..28185
                     /locus_tag="PKL42_01240"
     CDS             27835..28185
                     /locus_tag="PKL42_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HNU65721.1"
                     /translation="MFNLNTSISTSKTVAIATFATLLLSGITAQTAMAETKADSKVVV
                     AAASADPEFAKLDANGNGKISLKEAAKDKGVASVFDAIDANKDGDITAEEHAAYKAAS
                     AAPAAPVAAPAPAK"
     gene            complement(28470..29264)
                     /locus_tag="PKL42_01245"
     CDS             complement(28470..29264)
                     /locus_tag="PKL42_01245"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNU65722.1"
                     /translation="MQQYTDLLRHVLAHGNKKEDRTGTGTTSVFGYQMRFNLADGFPL
                     LTTKKVHLKSIIHELLWFLQGSTNIAYLKENGVRIWDEWADKQGNLGPVYGYQWRNWP
                     KPDGTHIDQIAQVVESIKKNPDSRRLIVSAWNVADVDQMKLPPCHAFFQFYVADGRLS
                     CQLYQRSADIFLGVPFNIASYALLTMMVAQVCDLQLGDFVHTLGDAHIYTNHMEQVHE
                     QLTREHRTLPQMHINPEVRDIFSFKFEDFTLENYDPHPAIKGVVAV"
     gene            complement(29264..30700)
                     /gene="thrC"
                     /locus_tag="PKL42_01250"
     CDS             complement(29264..30700)
                     /gene="thrC"
                     /locus_tag="PKL42_01250"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNU65723.1"
                     /translation="MKYISTRGQSPALQFSEILLGGLAPDGGLYLPETYPQITDADLT
                     AMRGMSYPELAFAILSRFVDDIPAADLKAIIDKTYRADVYGFTRKGQNAADITPTLKL
                     EDNLYLLSLSNGPTLAFKDMAMQLLGNLFEYVLTKTGKTTNILGATSGDTGSAAEYAM
                     RGKKGIRVFMLSPHKKMSRFQTAQMFSLQDENIHNIAVEGVFDDCQDMVKAVSNDAAF
                     KAAYKIGAVNSINWGRIVAQVVYYFKGYFAVTQNNAQKVSFSVPSGNFGNVCAGHIAR
                     MMGLPIDKLIVATNENDVLDEFFKTGVYRPRGSANTYHTSSPSMDISKASNFERFVFD
                     LVGRDAAKVRELWNSVDSGGAFDIKYLMPKLAEYGFVSGSSNHANRMQTIRETQAKYG
                     VVIDTHTADGLKVAHEYFNQTQADKNTPMLVLETALPAKFEDAITEALGHVPERPASL
                     EGIEALPQKFTVMKADAGVIKDFIAKNT"
     gene            complement(30749..>31021)
                     /locus_tag="PKL42_01255"
     CDS             complement(30749..>31021)
                     /locus_tag="PKL42_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNU65724.1"
                     /translation="VYLLASRKNGTLYTGVTSDLIKRVWQHKNDVVDGFSKKYNIHLL
                     VWYEVHDEIGSAIVREKQIKEWKRLWKLELIEKSNPTWKDLYSEIV"
BASE COUNT         8834 a         7137 c         6755 g         8195 t
ORIGIN      
        1 atcggttcct gtcacctcaa actcgtgccc atgcagatgt atcgggtgat ttgtcattgt
       61 taaattgcca attctaatac gcactttatc atttttgcgc accaccagtg ggtctatacc
      121 aggaaatgcc cggctattcc atgtccataa attaaaattt aacatcgtat tgatgcgtgg
      181 cttatagcta cctggctcaa tatcataagc attaagcaga aatataaaat ctctatccac
      241 cgccatgaat tttggacttt ttgggtgcgt cacccaacta cccatcaagc ccatcgccaa
      301 ttgcgtcatt tcatccgcat gcggatggta cataaacgta cccgcgcgtt tggcttcaaa
      361 ttcgtaaaca aaggttttac cgggtggaat ggcaggctgc gttaagccac ttacaccgtc
      421 catgccattg ggcaagcgct ggccatgcca atgcacactg gtgacttcag gcagacgatt
      481 tgtcacaaaa atacgcacac gatcaccctc taccacttcg attgtcggtc ctgggctttg
      541 gccgttataa ccccataaat gggcttgcat accaggggca atttctcgca ctacgggttc
      601 tgcaacaagg tgaaactctt ttatcccatt attcatccgc catggcaaac tccagccatt
      661 taatgtcacc acgggctgat agtgccgacc agtcgatgga tgcagtggcg cttgcgtatt
      721 agccttttct gcagaaacaa tgtctggaag tgcagctagc gcaactttgc tcaccgcgct
      781 taaagccaca cctgcacccg ctaatttaaa aaagtctcgt cttgtgaaat gttgttttgt
      841 tgtcatgatt attttccttc caaactggtt ggtttgccta taaacgcctt atttaacgcg
      901 gtttctgcca gccagaattc atttaagcta ttgatataag ccttcacgct cgcaacctgc
      961 gcacgggcat ctgcgaacaa ctcaaaagga ctcacgagca tgccgttata gcgcaattga
     1021 ttttcagcca atattttttg acgcaagggc acaatgtcgt cgcgataata tttcgccatt
     1081 tcataagtcg ccagataatg gctgtaagcc tcgctaattt cagattgcgc gttcacggcc
     1141 gtttgtgcgg cacgatgcac tgattgcata taaatagcct ctgcacgcgc gacttttgca
     1201 tctccccagt caaacaatgg caattcaaag gcaatatcca cgccattttt ataagcatca
     1261 ccacgtcggc cttccagcac tctggcaggg ccaatttcaa gcacgttaat aaaacgtgag
     1321 gttttggtca ggccaagttg tttagccaaa gccgctgttt ctaaacgtat ggcttgtaag
     1381 tcgagtcgct gctcaaacgc tgttttatct aatggctgta attcagaagc ggttttaggt
     1441 atatcgggca aacgtttttc taacttaagt tgttctgccg atacgcctag caatcgcgcc
     1501 agcgatgcat gcgcacgatt ctgcgctagc ttggcactgg tatattccag cagcgcatct
     1561 gcataaaatc cttgctctcg cgcttgctcc agcgtattcc aattacctgc ggccaccata
     1621 cgctgcgcca gcactgcact cgcttcggca gattctttta cctgaacgct ataacgcaca
     1681 agctcatttg ccgccaccgc attaaaatag gcggcccgtg tttcataagc caaactcagc
     1741 acttctattg ccactgcttg tttggttttc tcaaaatgac tacgctcgat ttctagcact
     1801 ttcggcatcg ccaataacga aaaaatatta aaggtaagtg cctgctcgat tttgtattcg
     1861 ccttgatggc gtgcgtagag catggagaat tttggatttg gcaatcgccc tgcttgcaca
     1921 acatctgcct ccgcaacacc caaagcgtat aacgaagcct gcaagccact gttattgagt
     1981 agcgcaacct gcactgcact atcagcattt aatggttctt gtaacaattc actgacgcgc
     2041 tctgccacaa tcgttttcgc gccttcattt tttggccaaa caacttcttg cttaagatga
     2101 ggtgcggtta aggcttttac atctgccaaa ccgccatcgt tagaaaaagt agcgcaacca
     2161 tttaataata ggcttagcaa tgcgctataa agcgcgaatt tataatattg catatcaact
     2221 ccatcggtat gaaacgatta ttcaggcctg ccagatcgct ctgcaaaagt aatattgtgc
     2281 aaagatggca ggcaggtttg ggcctagtga atcaaacgat gggtggtttt tgcggttgcg
     2341 cgcgaaatgg cgcgtggtag attttaacaa aagcgggaat aacagaaggt gtagcttgaa
     2401 gggttttaag agaaagcttc tcttgcacaa tcattgaaaa gcaggcaata caatgactac
     2461 aatgctgtga acctgcatgc gaatttgatt tttcgtgcgc ttgctcggct tgcatattct
     2521 ggcagtggac ggcatgactg gccgcttcgc ttacattttg ggcatcttgc tgaccagcaa
     2581 tcatgtcagc caccatcttg ctacttgcgt aaaccgtatc ggtaataaat actgtcagca
     2641 gcaacaaaat aaacgtgcgt aaaaatttga gtgaaaaaac ctgcataagc caattaaatc
     2701 acaaagacca agcaaggtaa actaacatgc ggaatacttt agtaccaaga ccacacatcc
     2761 aaacaaccag atgagctatt cttactacca atttcccaat ggccgtgttg cttgcattga
     2821 tgatgattgt gattttccgt cactcaatca agcgcttagt gaacccaatg gccttattgc
     2881 gattggcggt gacttatcat tgccacgttt gctcaacgcc tatcaacatg gcatttttcc
     2941 atggtttagc gagggtgaac ctattctttg gtggagccca aacccgcgca tggtgctttt
     3001 ccctgacgca ctcaagattt caaattcact caaaaaaaca ctcaaaaaca agccgtttga
     3061 cgtgcgcttt aataccgcat ttcaccaagt gattagcgct tgcagccaca ccccacgcgc
     3121 aggccaacct ggcacttgga ttactcaaga catcatcgag gcctactgca cactacacaa
     3181 tgcaggctac gccatcagcg ccgagtgctg gcaagataac acgctggtag gcggctgcta
     3241 cggcgtaaaa attggcaaaa tgttttacgg tgaaagcatg tttcaccttg taacagacgc
     3301 ttcaaaagtg gcatttgtgc acttggtgca gaaattaaag tctgaaggcg ttggcttgat
     3361 tgattgccaa atgaaaacag cccatttagc gcgctttggt gccaaagaaa tcagtcgcga
     3421 tgattttatt gacaaccttt ctagtcttac tttggcataa ctgcttttgt aggcgcgcaa
     3481 ttgattagta atgctttcat gctgtgcatg attagcagta cttatgccgt tggtattgcg
     3541 cgccagcaat aagggttata attttgccat gacatcgccc aatgacattc ctttacaaaa
     3601 gttgcagttt tacgtaacta cgccctacac atgcggctat ttgccgggca aactggcgca
     3661 aagtttaatc gccgccccgc agcatttggt gaacgcctct gtttataaca gcttgattca
     3721 acagggcttt cgccgtagcg gtaattttgc gtatcgtcca cattgcgaaa attgccaaga
     3781 atgcgtgcct gtgcgcattg tgctcaatga ttttgtacca acccgcagcc aaaaacgcgc
     3841 ttacaagcaa caccagcacc tcagcaccgc cattctgccg gtggattttc atgaagaaca
     3901 ttacgcgctt tatcacgcat atcagcaggc acgccatgca gagagcctag acaaagcgcc
     3961 agcacaaaat gacgtagagc aatatcgtaa ctttttatgc caaaccaacg tagaaagtgt
     4021 gttggtggaa ttcagagaaa acgggcaact gaacatggtg agtgtcatcg acatcgttga
     4081 cgatggcata tctgctgttt acactttttt tgatgcgacc aaacgcgcga gttttggcac
     4141 gtataacgta ctttggcaaa ttgaatgggc aaaacgttta gcacaccctt atttatattt
     4201 aggctattgg gtaaaagaca gccaaaaaat ggcctacaaa caaaactttc agccgcagca
     4261 aatgtactta gatggcgagt ggcgatcgtt aaaattgggt taattttcgc ttcatattag
     4321 cgcataaatt caacgcgtat tgatggtgct aaattgctta aattttaaga aattttaacc
     4381 caaacctttt tcgatgctct ggaagcctta taaaaaaagg ctcccagagc ataaaaattt
     4441 tctcgcataa aattaaagag aaaaacaagt aattaaaaaa cgtgaaacca gttgataatt
     4501 tcaaagcccg ccaagcaggt cgcggcaaaa atgttacaat cgcactttcg attttttaac
     4561 gtttaaaact gaaacaacaa tcgttttgaa aaaactagtc atctttggtg tgggtttaat
     4621 tggcggcagc gttgcccttg ctttaaaaaa agcaggccac gccacacaca tcgttggcgt
     4681 cggccgcagc cttgaaagcc tccaacgcgc tgtaaaatta ggcgtgattg atgcctttga
     4741 aactgacgtg cgcgcagcct gtgaaaatgc agatttaatt ctgattgccg cgccagttgc
     4801 tcaaactacc gacattctaa aaagcataca gccacatctc aacagcaaca cggtgattac
     4861 cgatgcaggc agcaccaaag gcgatgtatt gcgttgcgct gaaaaaattc ttggcccgca
     4921 actgcaccag tttgtaggcg gacaccccat tgcaggcgct gaaaaaagtg gcgtaaccgc
     4981 cgccacggcc gatttgtttt tgggtaaaaa tgtagtgctc accccaactg acaaaaccaa
     5041 tcaagatgcg ctcgcaaggg tcaaaaatct ctggcaagcc tgcggtgcca atgtgagcgc
     5101 catgcccgcc gaaacacacg atggcatttt tgcttcagtc agccacttgc cacatttgct
     5161 cgcatttgcg ctggtcgatg acattgccgc ccgccccaac gccgagcaat tgtttagctt
     5221 tgccgccagt ggttttagag acttcacgcg tattgctgga agccaccctg aaatgtggcg
     5281 cgacatcagc ttagctaaca aaaccgcgct tttgagcgaa attagcgcgt ttcaggcaga
     5341 gttatcccaa gttaaaacgc tattagaaaa ctgtgatggc gcaggtttac aagcgctatt
     5401 tgaacgcgcc agtgctgccc gtaacgcttg ggcaaaaaac caacaataaa gatacataaa
     5461 aatggaacaa ctacatctac cagcctacag ccgcgctcaa ggcacaatta aactacccgg
     5521 ctcaaaaagt atttccaacc gcactttatt actagctacc ctagcaaatg gcaaaacaga
     5581 tattcatgac ttgctcgcat ctgacgacac ctcacgtatg ctagaagccc tgcaaacact
     5641 gggggttaac ttagaaaatt tagccaataa cacttggctt gtcgaaggct gtgaaggtca
     5701 ctttcctaac aaaggcacac ctgaaaaacc ggtcgattta tttctaggta acgcaggcac
     5761 tgcatttcgt ccgctcaccg cagcactggc attttcaaac gggtattatc ttttacacgg
     5821 tgtaccgcgt atgcacgaac gcccaattgg ggatttggtg gatgcgcttc gccaagcagg
     5881 tgcgcaaatt gaatacttgg gcaatgaggg ctttccacca ttaaaaattt cacctgccaa
     5941 acttgattta agtcagtcca tcaaaatacg tggtgatgtc tctagccagt ttttaaccgc
     6001 cctactcatg gcgctaccgc tcaccaaaca aaatgcaacg attgaagtgg tgggtgaact
     6061 gatttcaaaa ccatacattg aaattacgct caatttaatg gcaaaatttg gagtgaaagt
     6121 tgagcgcaat ggctggcagc aattttctat ttccaactgt actgaatatc tgagtccaca
     6181 agtgtttttt gttgagggcg atgcttccag tgcatcgtac ttcttagctg ctggggcaat
     6241 tgctggagaa gtgcaagtca acggtttggg taaaaacagc attcagggag atgtccaatt
     6301 taccgaagcc cttgagaaaa tgggcgcaga aatccatttt gaagaaaatt ctgtcattgc
     6361 atcaaatgtt ggcaaactta aagccattac ccttgactgc aatcacatac ccgatgctgc
     6421 gatgacactc gcaattttag ccatatttgc taaaggtact accaccttac tcaaaatcaa
     6481 aagttggcgg gtaaaagaaa ctaacagatt aaaagcaatg gcaaccgaat tacgaaaact
     6541 gggagttact gtaaaatggg gtgatggata tattaaaatc acgcctccag aaaaactcac
     6601 cccaaacgcg gtgattgaca cttatgacga ccatcgcatg gccatgtgtt tttcattggt
     6661 gagtttaggt ggcgtgccga ttactattaa tgatcctaaa tgcgtgaata aaacttttcc
     6721 tgattatttt gacgcgttta aaaaaattgt cagttagcct tgttagtgta agccagcaca
     6781 aggcatggcg ctaaacgggg cagtgactga actagctgtt tttttctgct caaatatttg
     6841 ccaagcgcgc tcatgaaaat aaaacaccac cgtattgcaa gcaggctcaa taagcgccaa
     6901 caagccgcca atcacaaagc tgcctgtcag caaataggcc actaaaaagg caacggtaaa
     6961 gtgtaaaatt gcaaatgtga atgttttagt catgatgtac cacctttttt tactattgtt
     7021 tttagtatgt gcttcatgtg gtcattattg actcacttat aaaataaatc caattgattg
     7081 tttctctaat tacgataagt ttaacttatg aatttaccaa acaccgatat tcctgtcatc
     7141 gctattgacg gcccctcagc atcgggcaaa ggcaccgtgg cgcaactggt tgcgcaaaag
     7201 ctaggctttc attatttaga ttctggcgca ctttatcgca tcgtagcgta cgctgcacag
     7261 caaaaagata ttgcctgggg cgatgccttt gccgtaaccg aatgcgcaaa aaaactcacc
     7321 attacatttg ttggggagca ggttttactg accacgccag actttgcgca aaaagatatt
     7381 tccaatgaaa ttcgcaccga gacaatcggc aaaggagctt cacaggttgc cgtacatgcc
     7441 ccattaagag ctgcattggt cgaaatgcaa cacgcatttt gcaaatcgcc aggtttggtg
     7501 gctgatgggc gagatatggg tactgttatt tttcctgaag cgccactaaa ggtgttttta
     7561 accgcctcca ccgaagtgcg cgcacagcga cgctaccagc aattacgcaa taaaaaccag
     7621 cccgcagact atgaaagcat cttagcagac cttcaagagc gtgatgcgcg cgataaaaac
     7681 agagccgctg caccactcat catggcccaa gatgccattt tgcttgaaac cgaccattta
     7741 agtatttctg atgctgtaaa tgcagtttta gaaaattttc agcagaaaat gtacgaatag
     7801 tcttttattt ttcaatgttt tagttataat tcaattcttt gcaattgagt aatatttttt
     7861 tcgcccgcgc attccaaaga ttcgcggttt tttaatcacc ccactgctag gtgggattat
     7921 ttttttaggt atctttattt aatggcttct acttctaaat ctactacttc agcttcttca
     7981 ggcatggaat cttttgctgc gctttttgaa gaaagcttag ctcaccaaga aatgcgctct
     8041 ggcgaagtga tcaccgcgga ggttatctct gtggaccaag accatgtaat cgttaacgct
     8101 ggtcttaaat cagaaagcgt tatcgctgct gaagaattta aaaatgcgca aggcgaactg
     8161 gatgttaaag ttggcgactt tgtaaaagtg ttgattgaaa agctggaaga tggctttggt
     8221 tcaaccgtac tttcacgcga gaaagcaaaa cgcatggaaa catggttaga tttagaagat
     8281 gcaatgaatg aaggtcgcat catcaaaggt tttgttagcg gtaaagttaa aggtggttta
     8341 cgcgtttcag tgaatggcat catggcattc ttgcctggct cattggtaga tgtacgacct
     8401 gtaaaagaca caaccccttt tgaaaacaaa gaatgtgatt taaaagtcat taaaattgac
     8461 cgcaaacgta acaacattgt tgtttcacgc cgtgccgtga tggaagtcag cgcaggggct
     8521 gaccgtgaag cgctaatggg tacattagct gaaggcgcag ttgttaaagg tattgttaaa
     8581 aatattaccg actacggtgc gttcgtagac ttaggcggca ttgatggttt actccatatc
     8641 actgatttgg catggcgccg tgtaaaacat ccatcagaag tactgaccgt gggtgaagaa
     8701 gttgaagcga aaatcttgaa atttgatcaa gaaaaaaatc gtgtttcatt aggtatcaaa
     8761 caactaggtg atgacccatg ggttgcattg gcacgtcgtt acccagttag cactcgcttg
     8821 ttcggtaagg tttctaacct aactgattac ggtgcttttg ttgaaattga accaggtatt
     8881 gaaggtttgg tacacgtatc tgaaatggat tggacgaata aaaacattca cccgggtaaa
     8941 cttgcacaat taggtgacga agtagaagta atgattcttg aaattgatga agcacgtcgt
     9001 cgcttatcat taggtatgaa gcaatgccaa gcaaatcctt gggatgattt ctcagcaacg
     9061 cacgccaaag gcgacaaagt ttctggtcaa attaaatcaa ttactgattt tggtgtgttc
     9121 attggcttgc caggcggtat tgatggctta gtgcatttgt ctgatttgtc atggacacaa
     9181 tctggcgaag aagcgattcg taacttcaaa aaaggtgacg aactccaagc ggttatctta
     9241 gcaattgacg ttgataaaga gcgtatttca ctaggcgtta agcaacttac tgctgatcct
     9301 tcaggtgctt ctagcggttc tgacgataaa gctgatgcaa aaccaaaagc taaatctgct
     9361 aaaactaaag aagcagcaaa acctgccgat gatggcgcta ctgctggcac aacgaatctt
     9421 ggtgcgttat taaaagccaa attagacagc aaaaaataag ccgataatta ataaaatcct
     9481 agtaaagaaa taagggctca ataatgacta aatctgaact tatcaccctg cttgcagagc
     9541 gttttccgca attggtggtg aaagatgctg aattatcagt aaaagcgatt ttagatgcga
     9601 tgactgataa cctagccact ggcgaacgta ttgagattcg cggctttgga agctttagtt
     9661 taaattatcg cccacctcgc ttgggtcgca accctaaaac tggcagtaaa gtgcaagtgc
     9721 ctgaaaaaca cgtaccacac tttaaagccg gcaaagagtt acgtgaacgc gttgatttag
     9781 gctaaaaact agtattttga ttaatacgta aaaagtacca ataaaaacgg cagctaacag
     9841 gctgccgttt ttattgggtg tcagcaattt tcaggtaaaa tgtaattcaa gcatcattga
     9901 atagagtaaa acttaatgga attcgaattt tggtggttgt tggcattacc gcttttcttt
     9961 agcttaggct ggttggcagg tcgcatcgac cttaaacaac tgctttcaga atccgcagcc
    10021 ctgcccgcag cctatttcaa aggcctaaac tttctgatta ccaatcagca agataaagcc
    10081 attgaggcct tttcagaagc tgtacaggcc aataccgact cgctcgaact ccattttgca
    10141 ttaggcagcc tgtttagaaa gcgcggtgaa gtaaaccgcg ccatccattt acatcttcat
    10201 ttacttgaga aaaaagaact cgaaccgcaa caaaaactgg cggtcacggc agagctcgca
    10261 caggactact taaaggcggg tttactggat cgcgctgaag agctttttga gtcattaaac
    10321 gatgaccgct atcgccagcc agcgctacgc gccttacttg aaatctatgt gcgcgagcgc
    10381 gagtgggagc gtgccattaa agccgctaca gagctagaaa ggctttctgg tgtgccattt
    10441 agagttgaaa tttcacatta ccattgtgag ttagcggtta aatcgatgct tgccaatgac
    10501 attaaaacgg ctaaatttga actcgagcaa gcacttaacg ccaataaaaa ctgcgtgcgc
    10561 gccaacgtac tcttaggcga cttagaggca gaagcgaatg cacatcaagc cgctatttca
    10621 acttggaaac gcattgaatt tcaacaacct gaatatttag gcctgattgc gccaaaacta
    10681 cttaatagct atcgcgcgct caatcaaact gccgaaggct tggccttgct acaaacctat
    10741 ttgcaaacat acaaattatc tccccttcta aatgtgcttt ttgaggcaac tttggctgaa
    10801 gaaggcgccg aaagcgcggc caagcttgca cgtaacgagc tcattaaaaa accaagtttg
    10861 agcacattag accaactgtt acaggcgcgc acaattgtag aaaccaatca gcctgtgcaa
    10921 aaaactaaac tgacgagttc gcaagatacg cagttgatgc aacaagcggt tcgacatgcc
    10981 attggtaaca aaacagctta tcactgcgag caatgcggct ttaaagccaa ataccaccat
    11041 tggcaatgcc cagcctgcaa tgcatgggaa gcattacccg cagaacctac gaccatttaa
    11101 acgttttacg attcaatatg acccttgctc acgacgctaa aattcatgac ccaaaaatcc
    11161 atgacccaaa aattatagtc gcgctggatt atgcagatgc aaccagcgcg ctaaaacttg
    11221 ttgaacagct agaccctgcg ctttgtcgct taaaagttgg caaagaactt tttaccgccg
    11281 cagggccaaa acttgttgag caattggtac aggccaattt tggggtgttt ttagatttaa
    11341 aatttcatga cattcctaat acggttgcca aagcttgtac agctgcaagt caattagggg
    11401 tttggatgct taatgtacac gccagcggtg gcctggaaat gatgcaagct gcgcaacaag
    11461 ctgtgcaaag tagtgaagct aaaccgattt taattgcggt gactgtactc accagcatga
    11521 atcagcaaac gctcaatcaa attggcatcc acaccgactt aacgacgcac gtacagaacc
    11581 ttgcaaaact cacgcaacag gcagggttag ccggtgtcgt ttgttcagcc cttgaagcca
    11641 gcatgttgcg tcaagaactg ggcaatgact tttgcctagt cacgccaggt atacgcccgg
    11701 ctaatgtcaa ccaagacgac caatcacgcg ttgtcacccc ggcagatgca ctgaaaaatg
    11761 gtgcaagcta cttagtgatt gggcggccga ttaccaaagc catcaaccca cttaaagcac
    11821 tggaagccat acacgcagaa tgtaagcttt ttaacgctta gcgtaaattt accctctatt
    11881 gaatatggtt aaattgcgct atgatagcta cgcgtcgcta cagcggttat gttagcaaca
    11941 caatttctaa ctcacggaga attaaaatga aaaaaatgtt attggcattc atcgcttttt
    12001 taagctttag cttaagcgcc tttgctgcag tgaatatcaa caccgcaaca caaaccgagc
    12061 ttgaaacgct tgaaaatgtt ggtgaaacaa gagccaaagc cattattgaa taccgaaaca
    12121 aaaatggtaa gttcaaaagc ttggatgagc tagataaagt acctggtatc ggcgaaaaaa
    12181 cgcttgagag cattaaaaaa gatggcacaa tcagcggagc cactacagta acagcaccaa
    12241 acaaaaatac aaaaaatgaa aagccaatga aggaaaaagc aacgaaggct gaaaaagcag
    12301 ttgcaacaga caaacctgct aaagcggaaa aagcgagcaa agaagcaaaa tctgcaagca
    12361 aagaagatag cgtaaaagca gacaaagcag caaaagataa agaaaaagct gacaaagccg
    12421 ctgcaaaaga agcagaaaaa gaagctaaaa aagcnnnnnn nnnnnnnnnn nnnnnnnnnn
    12481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12541 nnnnnnnnnn nnnnaaatag ccccaacttt ggggctattt ttttagcacg aatttttacg
    12601 caaaagattg attaaaacag cgccaaatac tctcgagaga aaaattatgc tctgtaagcc
    12661 tttatttata aggctcgtag agcatcgaaa aaggttgtga taaatttagg ctaaaaataa
    12721 gcgtgttaag ggcatgcaaa tctgacgttt ttctgactaa attttaacca ttacttcacg
    12781 cgcattcctg cttgtgcgcc agtgtctgga ctcaaaatat aaggcccgcc acgctcatcg
    12841 ctggcagcta acaccatgcc ttcactcatg ccaaatttca ttttacgcgg cgcgagattt
    12901 gccaccatca ccgttaagcg acctttcaat gtttcagggt cgtaggccga tttaatgccg
    12961 gcaaatactt ggcgaggttt ttcttcacca atatcaagcg tgagtttcaa taacttttca
    13021 gcgccttcta catgttcggc attgacgatt ttagcaatgc gtaaatcaac tttggtaaag
    13081 tcatcaatac tgatgtagtc ggtttctacc gtttctgctt ttacctcatt ttgctgatgc
    13141 gccgcatggc gttgctcaga atgaggttgt ggtgcaggcg ttgcagttaa gttttcttta
    13201 ttggcttcgg tcatggcttc aatggctttg gggtcaatgc gggtaatgag atgttcatag
    13261 gcgttaatgg catgatttac tgccaatgat gcacccactg aagcccaagt taggggagca
    13321 atattcaaaa attgctcaat ttcagcagct aattttggca atacaggttt cagataaagc
    13381 gttaataaac ggaacagttc aagcgcatca gaacacactt cttgcaacgc cgcatcttgc
    13441 cccgcttgct ttgccatgac ccaaggggct ttttctgcca caaagccgtt agcaacatcc
    13501 gccaagcgca taatttcacg caaggctttg ccatattcgc gctcggcata tgcctcagcc
    13561 accacggttg cggccgcttt gatttctgcc acaatggcgt tgttttgcga tggctttaac
    13621 tggccagcaa agcgtttatt gataaaccct gccgtgcggc tggcaatatt gatgtactta
    13681 cccaccaaat cgctatttac ccgcgcgaca aaatcttcta agttgaggtc aatgtcttcc
    13741 atggtgctat tgagttttgc ggcatagtaa tagcgtaaat attcagggtt tttaatatgg
    13801 tctaagtaac tgcgggcggt aataaacgtg ccgcgcgatt tactcatttt ttcgccattg
    13861 accgttaaaa agccgtgggc gaaaatttgt gtcggtttac gatggccgct aaactctaag
    13921 gttgccggcc aaaataaggc atggaaatat aaaatgtctt tgccgataaa atggtaaagc
    13981 tcggttttgc tatctttcgc ccaatattca tcaaagttta aaccttgctt atcgcacagg
    14041 tttttaaagc tcgccatata gccaatcggc gcatccagcc acacgtaaaa atatttgcca
    14101 ggggcatcgg ggatttcaaa gccaaaatac ggcgcatcgc gtgaaatgtc ccaatcattc
    14161 aggccgtttt caaaccactc acccattttg ttggcggctt cgccttgtag cgtgggctta
    14221 ccttttatgg cttcgctgcg tgtccagtct tttaaaaact gctcacattc tgaaagttta
    14281 aaaaagtaat gctcggtttc tttgcgcaca ggggcagcgc ccgaaacggc agaataagcg
    14341 ttaatgagtt ccgttgggtt gtaagttgcg ccgcacacct cgcacgaatc gccatactgg
    14401 tctttggcat ggcattttgg gcattcacct ttaataaaac ggtctggcaa aaacatgttt
    14461 ttaacggggt caaaaaactg ctcaatggtt ttagtggcaa ttttgccatt ggcttttaat
    14521 gccttgtaaa tgccttgcga aagcactttg ttttcatcag aattggtgga gtagtaatta
    14581 tcaaattcca ccaaaaattc tttaaaatca gcactgtgtt cttggtgtac gctggcaatc
    14641 agcgcctcag gcgtaatgcc ttctttttca gcgcgcagca ttattggcgt accatgcgta
    14701 tcgtcagcgc acacataatg cacggtatgg ccttgcattt tttgaaaacg cacccaaata
    14761 tcggtttgaa tatattcaac caaatggcct aagtgaatac tgccgttggc atatggaagt
    14821 gctgaagtga cgagaatttt gcgtggttga ttggcagaag acataagttg aatgcatcat
    14881 ttaagctaaa acgctattct aacaaatgcc actggcttaa cccagcgctc tttgctgctt
    14941 ttattaaaat ttaaccggtc tatgatgcga tgcagtctac aatcagttaa aataccttat
    15001 ctttttactc aagttattta atcaggaatc atcgcaatgg ccatttcaga attactcgtt
    15061 caaagcacgc taaaagtatg tattgaccca aacacgggca aagactttgt gagcagcaaa
    15121 tcggccagaa acatcaagat tgatggcaat gacgtgaacg tggacattgt actgggctat
    15181 ccggcaaagt ctgtcatggc tgatattcaa aaattggtgg cagatgccct gctgggtttg
    15241 gctggcattg gccgactcac cgtaaatgtg agcagcaaaa ttgtcgccca caaagcacag
    15301 caaggcgtta cgctgttgcc taaagtaaaa aatatcattg cggtggcttc tggtaaaggc
    15361 ggcgtgggca aatcaaccac ttcagtcaat ttagcgctgg cgctggcggc cgagggcgca
    15421 accgtaggct tgctggatgc cgatatttac ggcccaagtc agccgcaaat gctaggcatt
    15481 agtggccgcc ctgaaagcac agatggcaaa agcatggagc cgatgcaggc gcatggcata
    15541 caagccatgt cgattggctt tttaattgac accgatacgc ccatggtgtg gcgtggcccg
    15601 atggtaacag gtgcgctgga gcaactattg cgcgatacca actggcatga gttggattat
    15661 ttagtgattg atttaccgcc aggcaccggt gacattcaac ttacgctggc acaaaaaatt
    15721 ccagtcacgg gggccattat cgtgacaacc ccgcaggata ttgccctgct ggatgcgcgt
    15781 aaaggcttaa aaatgtttga aaaagtgggc attccaattt taggcattgt tgaaaacatg
    15841 agcacgcaca tttgctcaaa atgtggccat gaagaacaca tttttggcgc aggtggtggc
    15901 gccctgatgg caagcgacta ccaagtggat ttgctaggca gtttgccgct cgacatgcaa
    15961 atccgcatgc aggcagacag cggccagccg acggtgatcg cacagcctga ttctaaaatt
    16021 gcgcatattt acaaagaaat cgcgcgtaaa gcggccatta aaattgccaa tgccagccta
    16081 gaccacagcg cgaaatttcc taatattgtg attcaaaaca cctagccata ggcatcatat
    16141 tcggcagcac atctatggcc atcaaaaagt tagtctcacc tattcaacag gcattttcat
    16201 gggttgtcgg cggcctcggc atactgtttg cactcaggtt tttgccagct tggctgcgct
    16261 ttgccgtgta tggcagtgcg gtgcttctgc tcatcgtgta tttcagctta tatgccatgg
    16321 tcgcgaggca tgcgtatgat ttgcttgcgc atccacccac acaaaaggcc gatgctgcgc
    16381 ttattttagg caacaaatct tacttgaacg gagcgcccaa cccttgcctc accggccgtg
    16441 taggtgccgg tctggcactc gcacaggcgg ggctggtcag caccttagtg atgagtggcg
    16501 gcaaagataa tgaagacggt gcgattgaag cgctcaccat ggagcagtac gcgttagcta
    16561 acgggtttaa agggcgcatt ttgttagaat cacgttcaag ctcaacgcaa gaaaattttg
    16621 ccttttccgc gcctattatg ctgaacggcc aaattaaaag cgtcattgtg gtctctgaac
    16681 cctaccacat gtggcgcgca caaaagctgg tgcaggctgg gcacctaggg caagattttg
    16741 aggtctttta tgccgcggca gccagccaat gctggacaga ttggggcatg ctatttaaag
    16801 gtgcattccg cgaaccgttg gcgattatca acaactatgc caaaggttat ttttaggcac
    16861 ttttagaaac gtaaaattaa agaaaatatc agctcaaaat acgccgagga aatttttatg
    16921 ccctgtgagc ctttgtttat aaggctcaca gagcatcgaa aaaggttgtg gctaaaataa
    16981 ggctaaatca aacgtttttt tctgtcgctt tagggcgcta tttttacttc ataacaatac
    17041 acgtccacac cattttttgg cgcgatgagc gttgcaggaa tcgccacccc tttgcgccag
    17101 tgatccaccg cattctgctt gccagcccct gaaacaataa aaatgacctg acgggtatta
    17161 tttaagcggt tttggctaat ggtaatgcgc tcggatggcg gcttaggcga gttaaacaca
    17221 ggcaccgcat ctgcactgtt atccacagca tgatttggaa aaagcgaggc ggtatgacca
    17281 tcctcaccta agcccagcag taccaaatcg aaagtcggcg cgccagccag cgtgtttgcg
    17341 taggtcgcag cgcccgccac cgggccaagt tctgctggaa tatcatgaat attttctttg
    17401 ggtatgccaa caaaatgcgt ccaaacttgt tctaccatca ggctattgcg gtcttcatgc
    17461 tcaggcggca agcaacgctc atcgccgtga taaatatgcc atttagacca atctaaataa
    17521 gcttcctgcg gtaaatcgcg caagaggcga tatgccgcgc gcggcgtgtg gccgccggcc
    17581 agtacaattg aaaactgacc gcgctgttga atggctgctt gcgcgctact cacaatcgca
    17641 tcgcataccg catgcctgag tccttcgata ctctcgaagg acttccagcg cgtattttgt
    17701 gggggtgttt ggtggttcat gttggtgatg gattaaatca taaaaaagtt aggccgaatt
    17761 tgacactatt ttgtgaaatt tgtcatgtat tttcatgcgt catatgcact ttagctccac
    17821 attttgtttg agttttttaa gctgaaacat tgtatgacct agcctccttg cattatcata
    17881 tagggctacg catagcgcca ttttcataac actaccaaca ggaaaaaatc catgctcatc
    17941 aaagacagtg aagttgtcga cttacaaaca cccaccggcg aaatgcgcgc gcatattttt
    18001 cgccccgctg cccctggcaa atatcctgcc attttaatgt tttctgagat ttttcaagtg
    18061 acagcaccga ttcgtcgcac ggcggccatg ctcgctgggc acggctttat tgtagcctgt
    18121 cctgaaatct accatgaatt tgaaccgcta ggcaccgtgc ttgcttatga tgaagccggc
    18181 accacgcgcg gcaatgaact caaaattacc aaaccagttg atgcctatga ttctgacgcg
    18241 cgcaccgtgt tagattactt aaaatcgcgc agtgactgca cgggccgttt aggcgtgatg
    18301 ggcatttgcg taggcgggca tttagcattc cgcgctgcca tgaaccctga cgtattggcc
    18361 actgtttgtt tttatgcgac agatattcac aaaaaagcgc ttggcttggg caaaaatgat
    18421 aactctctcg tgcgtgctgg tgaaattaaa ggtgagctat tacatatttg gggtcgtcag
    18481 gacccacacg tgccacttga aggccgtaat ttagtgaaag cccgcttaga tgaagttggc
    18541 acgcgttaca cttggcacga atttaatggt cagcacgctt ttatgcgcga tgaaggccac
    18601 cgttacgacc cagcacttgc acgtttaagc tgggatttat tgcttgagct atttaaccgc
    18661 cgcttaggct ggggcgattt agacattgaa aacgacggct cagccgctga aagccgccat
    18721 taaagcaatc tcagatacaa ccgttattgg tgtttatcgc aatcaaaacg gttctgacct
    18781 gttaattatt gaatcaaaat aaggtatcca tcaaaatggc taaaaaaatc gacccctgta
    18841 cccttgtatt atttggcgca agtggcaact tatctcgcgt taaattgatg ccgggcttat
    18901 ttagactaga cgctgcaggt cgcctgccag aaaaaatggc aattctatcg gttgggcgtg
    18961 gcgaagtatc acgcgaagca tggcaggctg acatcaaagg catgctggat gctaaattta
    19021 aaaatggcta cgaccaaaag gtatttgagc gttttattgc ccgtaatcat tatcaagcca
    19081 atccgccgac agacccagat gctttcaaaa aaatgaaaga aacactcagt aatgaggccg
    19141 tttttccgca aaacttggct tacttcttat cggtacgccc taccgacttt gcgcctgtgg
    19201 ttgagcaatt atccggcgtt ggcttgctgg atgaaagcaa atactggcgt cgtgtgttga
    19261 ttgaaaaacc gtttggtacg gatttagcat cagcgcaaga tttgcaagcg cgcttgacta
    19321 agcatcttaa agaaagccaa atttaccgta ttgaccatta cttgggcaaa actgcggtac
    19381 aaggcattat gctcacacgc tttgctaatg cgatttttga acccctttgg aataaagacc
    19441 acattgacca cgtgcaaatc accaataacg agattttggg cgtaggtgac cgaaccacgt
    19501 tttacaatgc cacaggcgca ttacgcgata tgttccaaag ccatttattg caaaccttgg
    19561 ccttaaccgc catggaaaaa ccaaaagatt tatcgccaga aagcatccgt gcggaaaaaa
    19621 tcaagctatt acaatccatc cgcccgattg atgctagcaa cctcaacaaa caagcctttc
    19681 gtgcacaata tgctgctggg cgtgtgtgtg tgggcgatgg tcatggtgaa aatgttgctg
    19741 gctacttaga tgaacttaaa cgtgatggga ttgaatccag caataccgaa acctatgccg
    19801 cagtcaaatt gtggattgat aatgagcgct ggaaaggcgt gcctttctac gtgcgcaccg
    19861 ccaaacgcat gcatgaaggc aatgtggcga tttcagtcaa atttaaaaaa tcgccgatgc
    19921 aattggtcga tagccaacat caaaactggc tggtgattag cattcaacca aaagaaaccg
    19981 ttaaacttga aattgaatca aaaatcccag gcttagacat tgccacgcgc acattaagca
    20041 ttgacgcgcc ccaacgtcaa tcgggtgatg aatcaattga ttcttacgaa accttaatgc
    20101 tgaacttaat ggaaggcgac ccatcgcaat acctgcacat cagcgaggta gaagcacaat
    20161 ggaaattggt tgacccgatt gttaaaacgt gggctgccga caacacgcca ctgcatcaat
    20221 accgcgcggg tgaccgtgac ccgaaagaat ctggcgtgat ttttgaagct gaagaccagt
    20281 tttggcgcta cagcattgaa ttaggtggcg ataagcacta attttttagc acacaatttt
    20341 gtgatgcgcc ttataatgcc caacttacag cgttgggcat tttttatatt ttttagagca
    20401 caaagatgag cataaaatca gacaaatgga tacgtaaaat ggcgcaagaa cacggtatga
    20461 tagagccgtt cgagccaaac caagtcaaaa tgaacgcggc tggtgagcgc atggtgtctt
    20521 acggcacttc aagttacggt tatgatattc gctgctcaag agaatttaaa ctgtttacca
    20581 acctcaacag caccatcgtt gaccccaaga attttgaccc taactcattt gttgaagtca
    20641 acgctgatta ctgcattatc ccacccaatt cattcgcact cgctcgcacg gttgaatact
    20701 ttcgcattcc gcgcaatgtg ctcaccgttt gcttaggtaa atcaacctac gcgcgctgcg
    20761 gcattattgt gaacgtcacg ccatttgagc ctgaatggga aggctacgtc acgctggaat
    20821 ttagcaacac cacgccatta cctgcaaaaa tttacgccaa tgaaggctgt gcgcaagtgt
    20881 tattctttga agcagatgcc gatgatgtat gcgaaaccag ttataaagac cgtggcggca
    20941 agtatcaagg tcaagttggc gtcactttgc caaaaattta agggctgaat atgaaattcc
    21001 gttttcctgt cgtcatcatt gacgaagact tccgctctga aaattcttca ggtttaggca
    21061 ttcgcgtact tgcaaaggcg attgaagccg agggctttga agtgctcggt gtcaccagtt
    21121 atggcgactt aacctctttt gcacagcaac aaagtcgtgc ctcagcgttt attttatcga
    21181 tagacgatga agaaattgta gaagaaaagc ctgaagccat tgagcaattg cggaaatttg
    21241 tcacagaaat tcgttaccgc aatgaagaaa ttccaatctt tttgcatggt gaaacgcgca
    21301 cttcacgcca tattccgaat gatgtgttac gtgaattaca tggctttatt cacatgaacg
    21361 aagacacacc tgagtttgtt gcccgattaa ttattcgtga agcaagaacc tacctcgata
    21421 gtttgccacc gccatttttt aaagcgctta cccattatgc ggcagatggc tcatactcat
    21481 ggcactgccc tggccattct ggtggtgtgg catttttaaa gtcacctgtt gggcaaatgt
    21541 ttcaccaatt ttttggtgaa aatatgttac gtgcggacgt ttgtaatgcg gtggatgaac
    21601 tcggccaact gcttgatcac acaggccctg tggcagcatc agagcgcaac gccgcgcgta
    21661 tttacaattg tgaccatctg tattttgtca ccaacggcac atccaccagt aataaaatcg
    21721 tctggaactc caccgtagcg ccaggcgaca tcgtcgttgt tgaccgcaat tgtcataaat
    21781 ctgtattgca ctccatcatt atgactggcg cgattccagt attcttgatg ccaacccgca
    21841 accactttgg cattatcggg cccattccaa aatcagaatt tgcctgggaa aacattcaaa
    21901 agaaaatagc gcgcaaccct tttgcaactg ataaaaatgc taaaccgcgt gtattgacca
    21961 ttacacaatc cacttacgat ggcgtacttt ataatgtaga agaaatcaaa gaattgcttg
    22021 atggcaaaat cgatacgcta cactttgatg aagcatggct accgcatgca acgtttcatg
    22081 acttttatgg tgactaccat gcgattggtg ctgaccggcc gcgctgtaaa gaaagtatga
    22141 ttttttcaac gcaatcaacc cataaactgc tggcgggcct aagccaagct tcacaaatct
    22201 tagttcaaaa tgctgaagaa attcagcttg accgcgatgt ttttaacgaa gcgtacttga
    22261 tgcacacctc caccagcccg caatattcga ttatcgcgag ttgtgacgtg gccgccgcta
    22321 tgatggaagc accgggcggc accgtgctgg ttgaagaaag cttaatggaa gcactagatt
    22381 ttcgccgtgc catgcgtaaa gtggatgaag aatggggtac cgactggtgg ttcaaggttt
    22441 gggggccaac cgatttatct gaagaaggct tggaagagcg cgatgcctgg atgctgaaag
    22501 ccaacgaggc ttggcacggc tttggcaatc tatcggaagg ctttaacatg ctggacccga
    22561 ttaaggccac catcatcacc ccgggcttgg atattcatgg cgatttttct gaaaacaatg
    22621 gtattcccgc cgcgattgtt accaagtatt tggctgaaca tggcgtgatt gtagaaaaaa
    22681 ccggcttata ctcatttttc atcatgttca ccatcggcat caccaaaggc cgttggaaca
    22741 cgatggtagc ggctttgcag cagtttaaag atgactacga taaaaaccag ccattgtgga
    22801 aagtgttgcc tgagttcgta caaaagcacc cacgttatga acgcgtaggc ctcaaagatt
    22861 taagcacgca aattcatgct gtgtataaag ccaatgatgt ggctcgatta acaactgaaa
    22921 tgtatttatc tgacatggta cctgccatga agcccacgga cgcgtttgca aaaatggcgc
    22981 atcgcgagat tgaccgtgtg ccgattgatg atttagaagg ccgcgttact gccgtattgc
    23041 tcacacccta cccgcctggc attccgttgc tgattccggg ggaacgtttt aacaaaatca
    23101 tcgtggatta tttaaagttc gcccgcgaat tcaatgaaaa attcccaggt tttgaaacgg
    23161 acaaccacgg cttggttaaa gaaatgatcg atggaaaacc ggtgtattat gttgattgcg
    23221 ttgcgcagta agtaatcatt agtaatgctt catgcttgca tgattagcag tacttatgcc
    23281 cgtggtatta gtaatgcttc atgcttgcat gattcgcttt attgatgtgt gggattgatg
    23341 caaccttttt cgatgctctg caagccttat aaataaaggc ttccagagca tcaatttctt
    23401 ctcggcgtaa atgactgcat ttataagcgg tttatacgcg ctaatttttt agttttttct
    23461 tataaatacc aaatcccgat acgccatgcc attggcagta atatgctgct tttgttcagc
    23521 cagttgccag tggttaaagt ctacttctgg aaaaaaagcg tcgcctataa catctaaatc
    23581 aacttccgtg atgtagagtt tggtcgcctt agccaaaccc gtttgatata actctgcacc
    23641 gccaattaaa aacggttctt catcaccctt gcaaagtgca atggcagcat ccagcgaatg
    23701 ggcaatcagc gcgccttcaa cgtgataatg cggatttcgt gtcacaatca ccgtggtacg
    23761 gtttggtaat aaacggccga gcgattcata agttttacgc cccatgatga tatgatggcc
    23821 agttgtgagc gcacgaaaac gctttaaatc ttcaggcaaa tgccatggca aggtgttgtt
    23881 taaaccaatc acattgtttt ttgcgcgggc tacgatgagt gaaagtgcag tcataggcgt
    23941 attatactag gcttgctgcg tattttgtgt cgcatgcatt ttactggcct catacacatt
    24001 agatgccgct aaaataacgt ttatcaagtt aaaacattaa cccgacaaat ttgcaaatca
    24061 cttacccaaa atctttttta aaactattgt tgattggctt tgcgcttgtc atactgccgc
    24121 tgattgcggc cttcgtcaac gccaacctac aatttaacaa gctcacgcag caaagcctgt
    24181 ttaacatgtc gcaggccgta gaaaccacgc gtgcaagcca aattatgctg gaagaactgg
    24241 cagtgatgga gcggagcgcc cgacagtact ttgtattgcg cgaccaagta ttgctaagca
    24301 actttaatca ggcacatgaa cgactaggtg ccgcactcaa tatcttattg ctacaaccca
    24361 ttgcattgcc gcaacacaca gatttgatgt catttacgcg gcaagaaaat gatttgtttc
    24421 aatctattac gcaggcacag cacaataaag cagattttga tgacagcatg gtattgaatt
    24481 ttgccacatt aacgaactta gccaatcaaa tttccacctc caataatgca ttgattgacc
    24541 aagaggctgt cacctttaaa gttcgcgtgt ctaaagtaaa aaaagccttg ttttggcaaa
    24601 tgctgaccat ttttccgctg gtattgatta tagccgcact cattacctgg atgattgcgc
    24661 gcccaatccg ccgcatggat gccgccatca accagctagg ccaaggcgat tacgagcatg
    24721 tgatacacat caatggccct ggcgatttac ggcaattggg cagtcggctc gattggttac
    24781 ggattgcact taaagatttg catcagcaaa aacaacgttt tttacagcaa gcatcgcatg
    24841 aactcaaaac accgctcacc gccatccgtg aggccagtga attgctccat gatggcgttg
    24901 cgggtaatct gaatacgcaa caaaatcaga ttgttaccat tttgcgtgat agcagtttaa
    24961 ggctacaaag gatgattgag aacttgctga aatacactga aattcagttt aatgctgcaa
    25021 aaagtaatgt ttcacaacaa gcgcttaacg ttgtaattca gaaagtgttg caagcctatg
    25081 cgctcaccat cgcacataaa aacattgatg tgcaacagtc gattcccgat atgcaagtac
    25141 aactcgatat tgaaaagctg agcactgtac ttgataattt aatttcaaat gcggtgaaat
    25201 atacccctga acatggcaaa atcagcatcc acgccacccg cacgaaaaat gatttggaga
    25261 ttgcaattaa agataatggg cctggaatac ctgatgcgaa tcaaaaagat ctgttcagcc
    25321 cgttttatca tggtgagcaa gccagtgata gccttgtggc gagtagcggt ttaggcttat
    25381 tcattgcaaa agaagcagcc agtgcgcttc aaggtgagct acgctacatt acctcagacg
    25441 caggcgcgca ttttttagtg cgaattccac tatcgtctat gcaacccaac catgaacaac
    25501 ctactgaagt gacaaatcga tgaaaaaaat acaatggaat tcaatggtag tgttatgcat
    25561 aatgacgatc gcttgcactt cacaagccac aaagccaagt aaccacaatc ataaaaccca
    25621 agcctctctg caggaggcaa ccccaaatgg gattaaggca aatgaattca agtctgctga
    25681 agttaaacct gttgaaattg ccaaaccagt tgacaacaaa ccaagtttga ctggtttatc
    25741 taacaccact tctaggcctc aggtcagcca ttacttgagc tttgccgatg acttttcaaa
    25801 cttcactgca gaggcgcaaa aacaagtgct gagcaatacc ctacaagcgt tcgctaaagc
    25861 gcctaatgat attgagcaac gcatgcgact tgtcttgatt tacggcctac caagcagcac
    25921 ccttcttgat tctgccaaag cacagtcaca attgcagcaa atactgcaag aaagtgtttt
    25981 acccagtgag caacaagcct ttgccaattt gctgtttgat tatattgttg cggtgaacaa
    26041 agccagcaaa atcagtcgtg aagagcaaaa aaagctcgaa accatgcaac aaaaacaaga
    26101 agcattgcaa acaaagctag aaaatacaca gcaaaaactt gaagccgcgc aacagaaaat
    26161 tgatgaatta aaaaacattg aaaaatcgat ggggcaacga gaagttgctc ccaaaaaata
    26221 gccttttcta ctcatgagcg cataaataat ggccaatcca attttaattg ttgatgacga
    26281 tgcagacatt ttaaagctac ttgaaatgcg gcttacggca agcggctacc cggtcattgc
    26341 agctagctct gctcaacaag cgcttacgct atttggcatg cacccgattg cactggtgat
    26401 tagcgattta cgcatgcctg aaatggatgg ctttgccctg tttgaagcca ttcatcggct
    26461 cgatagtacc attccattta ttctactcac cgcgcatggt tcgattcctg aggcggtaca
    26521 tgccacgcaa caaggcgtat ttagcttttt aacgaaacct tttgaaggca aagccttact
    26581 agaacaagta gctcaggcgc ttagagtttc accgcatcac cctaaccatc agcaagaaaa
    26641 tgacacctgg cgttccagca ttatcagtgg tagccagcaa atgcaaagcc tgatggcaca
    26701 ggccaaactc gtggcggaat cagacgccag tgtgctaatt caaggagaaa gcggcactgg
    26761 taaagaacta ctggcacagt ccattcatca cgccagccca cgccgtgaac atccatttgt
    26821 ggcgattaat tgcgcggcca tccccgaggc cttattggaa tctgaactct ttgggcataa
    26881 aaaaggcgcg tttaccggcg ccacgcgtga ccatcagggg ttgtttcaaa ccgcagatgg
    26941 cggcacgctg tttttagatg aaattggcga catgccgatg agcatccaag ccaaactttt
    27001 gcgtgcatta caagaacgta tgattcggcc tgttggcgca agcacgcaag cgcccatcaa
    27061 tgttcgcgtc atttgtgcaa ctcacaaaaa tctaatttta gaaatgcagg aacatcgctt
    27121 tcgtgaagac ctatactacc gcatcaatgt tgtcagcctc gagattccgc cgctagccaa
    27181 aaggcgcgaa gatattccat tgttagccaa tcactttttg gcggttttaa ataaaaaata
    27241 tcataagaat attaacggat ttgcgccgga agcttttgag ttgctgatta gtgcgccttg
    27301 gccgggtaac gtgcgacaat tacagaacat tatggagcaa gtagtggtgc tctctaccac
    27361 gcccatcatc accagcggct tggtggagcg agccttacag gataatattt ccagcatcat
    27421 cacatttgat gcagcacgca aaaactttga acagcagtat ctcatcaatc tgctgaaagc
    27481 cacacaaggc aacgtgacgc aggccgcacg aattgcagat agaaatcgga ccgaattcta
    27541 taaaatatta gaccgccacc atatcaaccc agctcttttt aaaacgcaag taactgatga
    27601 ataaggctta aatgggtttt aatttgagcg tcgcttttca gcgacaataa ataaccttaa
    27661 taatcatcac cctactaaaa cccgctaatt tttgtcgctt atttgagaca aaaattcatt
    27721 cctgaatttg cattaaacta tgcatctaat tgatttaatt cgttaattta aactggcaca
    27781 gcgcttgcta tataagaagt gcagtaactt tttaattact aaggaattca atccatgttt
    27841 aatttaaata cttcaatctc tacttcaaaa actgttgcaa tcgcgacatt tgcaacctta
    27901 cttttaagcg gcattacagc gcaaactgca atggcggaaa caaaggctga cagtaaagtt
    27961 gttgtggctg ccgcaagtgc tgacccagaa tttgcaaaat tagatgccaa tggcaatggc
    28021 aaaatttcat tgaaagaagc cgctaaagac aaaggcgttg cttcagtatt tgatgccatt
    28081 gacgctaaca aagatggtga cattaccgcc gaagaacacg cagcttataa agctgcaagc
    28141 gctgcaccgg ctgcaccagt tgctgcgcca gcaccagcga agtaattttc atttgattgg
    28201 ttaagtttta ccctgcaatt acctccccct gatgattgta ggtcctccgc cccgctgcct
    28261 caagtagcgg ggcatttttt tcctctacat atcatgtaaa acaggcaaca atttccccga
    28321 gaaaaaataa atgccctgcg agcctttatt tataaggctt acagagcatc gaaaaaggtt
    28381 gtagatgaaa agtgtatatt ttaaggtgat ttacgcatac aaaatcaatg cgcgaagttt
    28441 gctttgctgc gctgttaaac tgcaataagt taaaccgcaa caacaccttt gatggccgga
    28501 tgtgggtcgt aattttccag cgtaaaatct tcaaacttaa agctaaaaat atcacgcact
    28561 tcagggttga tgtgcatttg tggcaacgtg cgatgttcac gcgtgagttg ctcgtgcact
    28621 tgttccatat ggttggtgta aatgtgtgca tcgccaaggg tatgtacaaa atcacccaac
    28681 tgtaaatcgc acacttgcgc caccatcatg gtcaacaagg catacgaagc aatgttgaac
    28741 ggtacgccta agaaaatgtc cgcgctacgt tggtaaagct gacaactcaa gcggccatct
    28801 gcgacataaa actgaaaaaa cgcatgacaa ggtggaagtt tcatctggtc tacgtcagcc
    28861 acattccaag cagaaacaat caaacggcgt gaatctggat tttttttaat actttcaacc
    28921 acttgtgcaa tttggtcaat atgcgtgcca tcaggcttag gccagttgcg ccattggtaa
    28981 ccatacaccg ggcctaaatt gccctgctta tcagcccatt catcccaaat acgcacgccg
    29041 ttttctttta aataggcgat attggtactg ccctgcaaaa accaaagtaa ctcgtgaata
    29101 attgacttga gatgaacttt tttagtcgtc aacagcggaa aaccatccgc caaattaaag
    29161 cgcatttggt aaccaaacac tgaagtcgtg cccgtgccgg tgcggtcttc ctttttattg
    29221 ccgtgtgcta atacatggcg tagtaagtca gtatattgtt gcattaggta ttcttcgcaa
    29281 taaaatcttt aatcacacca gcatcggctt tcattaccgt aaatttctgc ggcaatgctt
    29341 caatgccttc caagctggcc ggacgttctg gcacatggcc taacgcttca gtaatcgcat
    29401 cttcaaactt agctggcaag gccgtttcta acaccagcat tggtgtattc ttatcggcct
    29461 gggtttgatt aaaatactca tgcgccactt ttaaaccatc cgccgtatgc gtatcaatca
    29521 ccaccccgta tttagcctgt gtttcacgta tggtttgcat acggtttgca tggttactgc
    29581 taccagatac aaaaccatat tctgctaatt ttggcattaa atatttgatg tcaaacgcac
    29641 cgccactatc gacgctattc cagagctcgc gcactttggc ggcatctctg ccgactaaat
    29701 caaacacaaa gcgctcgaaa ttggacgctt tgctaatgtc cattgaagga ctggacgtgt
    29761 ggtaagtatt tgccgaaccg cgtggacgat atacgccagt tttgaaaaac tcatccaaca
    29821 catcattttc attggtggca acaatgagtt tatcaatcgg caagcccatc atgcgggcaa
    29881 tatggccggc acacacgttg ccaaagttac ctgatggcac ggaaaaactc actttttgcg
    29941 cgttattttg tgtaacggca aaataaccct taaagtaata caccacttgc gcgacaatac
    30001 gaccccagtt aatggagttc accgcaccga ttttgtatgc tgctttaaat gccgcgtcat
    30061 tactcaccgc ttttaccata tcttggcaat catcaaacac gccttcaacg gcaatgttgt
    30121 ggatgttctc atcttgtaga ctaaacattt gcgcggtttg aaagcggctc atttttttgt
    30181 gcgggctcag cataaacaca cggatgcctt ttttaccgcg catggcgtat tccgctgcac
    30241 tgcctgtatc acctgaagtt gcccctaaaa tattagtggt tttgcccgtt ttggtgagca
    30301 catattcaaa caaattgccg agcaactgca tggccatatc tttaaacgcc aatgttgggc
    30361 catttgaaag gcttaataaa tacaaattgt cttctaactt aagtgtaggc gtaatatctg
    30421 ccgcattttg gcctttacgc gtaaaaccat acacgtctgc gcggtaggtt ttatcgataa
    30481 tcgcttttaa atcagccgct ggaatatcat ccacaaaacg cgacaaaatc gcaaaagcta
    30541 actctgggta tgacatgcca cgcatcgccg ttaaatcagc atcggtgatt tgtggatacg
    30601 tttcaggcaa gtacaaaccg ccatctggcg ctagcccacc caatagaatc tcactgaact
    30661 gtaatgcagg tgattgcccg cgcgtgctga tatatttcat ttatttattg tttcccactc
    30721 tgtcattccg acgaaagtcg gaatccagtt acactatttc actataaagg tctttccatg
    30781 ttggattgga cttttcgatc agttccaatt tccaaagccg cttccattct ttaatctgct
    30841 tttcacgaac aattgcgctg ccaatttcat catgcacttc ataccacacc aacaaatgga
    30901 tgttgtattt tttactaaaa ccgtccacca cgtcattttt atgttgccaa actcttttta
    30961 tcaaatcaga agtaacaccc gtataaagcg taccattttt ccgacttgcc aaaagatata
    31021 c
//
LOCUS       DAOQSI010000008        28781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_987_length_28781_cov_5.613869, whole genome shotgun sequence.
ACCESSION   DAOQSI010000008 DAOQSI010000000
VERSION     DAOQSI010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 28781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28781
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_987_length_28781_cov_5.613869"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="PKL42_01260"
     CDS             complement(<1..493)
                     /locus_tag="PKL42_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNU65725.1"
                     /translation="MIANNINKVVLITGGAKRVGAAICRQLHAQGANLMIHYRSSTSE
                     ARALQAELNLQRANSVAIIQADLLDIVNLPHLIGQTIHHFGRLDVLINNASTYYPSEI
                     GHIDEAKWHDLMGSNLKAPLFLSQAATPELRKNHGCIVNITDMHVERPKKGYIVYSVA
                     KAGL"
     gene            536..1720
                     /locus_tag="PKL42_01265"
     CDS             536..1720
                     /locus_tag="PKL42_01265"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNU65726.1"
                     /translation="MSTLPKPAPEAEKHSEQLVLKIQDEIQSAGGWLDFARFMQLALY
                     ATGLGYYSAGSQKFGDIKKGGGDFVTAPQISPLFAQTLANQMIQVLQLTQGSVLELGA
                     GTGKLAADCLLTLAKLNGLPERYFILEVSDHLRQVQLETLSQTLPQDVLQRVEWLNEL
                     PQQFAGIVIGNEVLDAIPVNIVHKHANGIEVCGIAVVNHKLVWQNRPINQHGTPSEKS
                     LFEAVTNLNLPEGYTTEICPAASGLMTSLANMLKNGAILMIDYGFSAREYYHPQRNQG
                     TLMCHYQHYAHTDPLIYVGLQDITAHVDFTSIAHAAVKGGLSLSGFCSQAQFLMNCGI
                     LTLLSEVSPTDMARYAPLAAAAQKLLSPAEMGDLFKVIAFTKEIDTPLMGFLTGDKSH
                     TL"
     gene            1766..2512
                     /gene="trmB"
                     /locus_tag="PKL42_01270"
     CDS             1766..2512
                     /gene="trmB"
                     /locus_tag="PKL42_01270"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNU65727.1"
                     /translation="MTNDIKTEHQSLQEIPKDEKSTNDELKHRRIRSFVLRQGRVTKG
                     QERALEIGWPKFGIQYAAKPIDLNTVFQRQASAKILEIGFGMGETTAKIAQTLPHCDF
                     LAAEVHTPGVGALLKLIEAHALTNIRIIQHDVVEVLQNMLADASLDGVHIFFPDPWHK
                     KRHHKRRLIQVEFVKLLCTKLKVGGYLHVATDWQEYAEWVLAVLSAETQLTNTAENYA
                     PKPSYRPLTKFENRGLKLGHGVWDMVFTRV"
     gene            complement(2471..3112)
                     /gene="yihA"
                     /locus_tag="PKL42_01275"
     CDS             complement(2471..3112)
                     /gene="yihA"
                     /locus_tag="PKL42_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNU65728.1"
                     /translation="MPLFQNATFFISAHHLRDLPPASGIEVAFAGRSNAGKSSALNTL
                     ANHNRLAFVSKQPGRTQLINFFTLGNDRHLVDLPGYGYAKVPEAMRAHWQNVLARYLS
                     ERSSLGGLVLVMDSRHPLTPLDRQMLDWFCPSGKPVHVLLTKSDKLSRGEASLTLNKV
                     RKELVETWGNACSIQLFSSLKKLGVEEAEKVIGKWLFDEDSNSGEDHIPNTVP"
     gene            3260..3892
                     /locus_tag="PKL42_01280"
     CDS             3260..3892
                     /locus_tag="PKL42_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNU65729.1"
                     /translation="MQFRHRVGLNSVAASLTGLMLSLAFSLPAQAADAVNSAEETAKN
                     VCSACHGVDGNSAITLNPKLAGQHPEYLQKQLTEFKSGKRANAVMAGIAGALSDEDMR
                     NLAAYYSKQSLKLGQAASNGKGSLGEKIYRGGVAATNVPACASCHGANGAGIPKQFPR
                     VNGQHTDYTLAQLKAFRSGERANAPMMMVISAKMTDAEMAAVSDYIQGLR"
     gene            complement(4108..5820)
                     /locus_tag="PKL42_01285"
     CDS             complement(4108..5820)
                     /locus_tag="PKL42_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNU65730.1"
                     /translation="MLSLSKIYRRMFDDTDRLMALMLLSLHALLIWGDFSQFFSALFL
                     CHFGFFLLWQPIFHHNQKLSWSSILVVLIGATFALYYMNWWITAFWIAGLFALIGGRI
                     ISGETTRARLPNILAATYLLAVLLLWVVPKLLNAKDDLIAAEFLLTYLLPILPLTILF
                     LSAKNKPVSPAQNIDFFYTLLIYLVTLIIILGGFAIGVTWQVHYVKLLIYVVLGLAVT
                     LVMLSWLWNPSETFSGLELLMSRYLLSLGLPFEEWLRKIAMHADYELTASDFLEASMR
                     ELAALKWVSGVLWEVDGAKHQAGFATPHISTFSFQQLHLTLYSRWQFSPAMYLHVQLL
                     TQIMSEFYEAKCREETISQNTYMQTFYETGSRLTHDIKNILQSLGTLTSAAEQTSDAE
                     GDIRLIELIKKQLPRLNQRLAATLTKLERPSVEKKRQAKVATWWKSFRQLNSNHQIQF
                     ESPAVLPKADIDPDVLDSVVDNLVQNALEKAKLEANTQIKVTLMPSNHFCLEVTDTGS
                     AMPQDIAEQLFKSHVTSKNGLGVGLYHAAQQASQAGYELSLVDNVNGEVRFRLEMLAP
                     EQAI"
     gene            6151..6792
                     /locus_tag="PKL42_01290"
     CDS             6151..6792
                     /locus_tag="PKL42_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNU65731.1"
                     /translation="MKKQQTGFTLIELAIVLVIIGLLLGGVLKGQELINSAKVKNMAS
                     DLKNIQIYVYSYQDKFKAIPGDDKSAITHVGAAVNGDGDGEIDGAWNATSATESYYFW
                     EHVRRAGIASGSTDSATTNTTYLPTNNEGGPVGVQSKPQNTITGLSGSYAVCSGGIVG
                     KIAKQLDSQIDDGDTTKGSVMVAATVTGTTAASPVATTATDFDSTKYVLCLAF"
     gene            6986..7786
                     /locus_tag="PKL42_01295"
     CDS             6986..7786
                     /locus_tag="PKL42_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNU65732.1"
                     /translation="MTNNIVEIDNLSFGYKGRLLHKGINMVFPRRKVVAIMGGSGSGK
                     TTLLRLIGGQIKPTKGEVRVEGQVVHQQDRDGIFKLRREMGMLFQHGALFTDLSVFDN
                     VAFPMRELTDLPESMIRDLVLMKLNAVGLRGAHTLTPPELSGGMARRVALARAIALDP
                     NIIMYDEPFAGLDPISMGVICDLIRSLNDALGATSIIVSHDVKETFLIADYVYFVANG
                     EVAAEGTPHDLMQSTLPFVHQFVHGEKDGPVPFHYAAPDYQKELMQNV"
     gene            7779..8582
                     /gene="mlaE"
                     /locus_tag="PKL42_01300"
     CDS             7779..8582
                     /gene="mlaE"
                     /locus_tag="PKL42_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HNU65733.1"
                     /translation="MSKINLVNKLLSGLRMTGHRVIEQIWRLGAGARLFFLTLTYSAE
                     SFRRPHLIVREMYFTGVMSLIIILVSAFFVGMVLALQGYNTLQKYGSSESIGVLVALA
                     LIRELGPVVTALLFAGRAGTAITAEIGLMKATEQLSAMEMMAVNPISRVIAPRFWAGV
                     FSMPILVCLFSMVGVLGGHLVAVAQIGVDVGAFWSQMQANVEFADVTNMLIKSFVFGV
                     ACTIIALFEGYDAPPTAEGVSHATTRTVVTSALAVLGLDFILTSFMIVA"
     gene            8639..9184
                     /gene="mlaD"
                     /locus_tag="PKL42_01305"
     CDS             8639..9184
                     /gene="mlaD"
                     /locus_tag="PKL42_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HNU65734.1"
                     /translation="MSRTTIDLWVGVLVVLGIAAFFGLAMKVGNLTTSSLGETYSVTA
                     EFENIGGLKPRAPVKSAGVVVGRVDSITFNSKNYAAEVTIKIDQRYPFPTDTFANIYT
                     AGLLGEQYIGLEAGGDDVDLKNGDKIAKTQDAIVLEKMISQFLFSKASETEDKNSKNE
                     KDAVSDSGENAKSTELGDPPF"
     gene            9249..9866
                     /locus_tag="PKL42_01310"
     CDS             9249..9866
                     /locus_tag="PKL42_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNU65735.1"
                     /translation="MKILLKWMIGASLLANLLVASAANAELAPDVLVKQTADDVLNII
                     KNDKEIQAGNQQKIYALAEEKILPNFDFDRVSRMVLGKSWRAATPEQQASFQKEFRSL
                     LLRTYSVALGKYRDQTIEYKPMRAEPDAKNVTVKTQILQNGGQPISVDYSLVKGANGW
                     KVYDIVIESVSLVTNYRSQFSQEIRQNGIDSLNKKLAEKNKSAGV"
     gene            9887..10198
                     /locus_tag="PKL42_01315"
     CDS             9887..10198
                     /locus_tag="PKL42_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNU65736.1"
                     /translation="MENSTLSSITQHANTWSVAGPMVVDTVNKLLLESAKFTNNASAF
                     EVNFSKVTDVDTAALSLMMEWQRRAVALNKKVVFTHLPANLTSLASLYGVTEFIPMVA
                     S"
     gene            10352..11287
                     /locus_tag="PKL42_01320"
     CDS             10352..11287
                     /locus_tag="PKL42_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNU65737.1"
                     /translation="MKPAIQFNRVHKNFGELQALNGIDLTIPQGEFFGLLGPNGAGKS
                     TLINILAGLLKPTYGTASVMGHDVVSDYQAARMALGVVPQELVFDPFFNVREMLRFQA
                     GYFGRGRENDAWVDEILEGLGLTDKAHTNMRKLSGGMKRRALIAQALAHKPPVIVLDE
                     PTAGVDVELRQMLWEFIKKLNREGHTIVLTTHYLEEAETLCSRVAMMKQGEIVALDST
                     ANLLNKFAGKNLRLVLGNAPLPAPLLSMLKSGEHGVYMLALSHLSQVEFALSELRKAN
                     VQIEDMQLIEPDLEDVFMSLVGSASHRNDDLIIQS"
     gene            11294..12070
                     /locus_tag="PKL42_01325"
     CDS             11294..12070
                     /locus_tag="PKL42_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNU65738.1"
                     /translation="MSLMNQKWRGTYTLFKKEVLRFWRVAFQTVASPVLTALLYLLIF
                     SHVLASHVQVYENVPYTAFLIPGLIMMSLLQNAFANSSSSLIQSKVMGNIVFILLTPL
                     SYLQFYMALLAAAMIRGLFVGFVIYLVALFFVDLPIVHVGWILTFAVLGSALLGTFGI
                     IAGIWADKFDQMAAFQNFVIMPLTFLSGVFYSIHSLPPFWQVVSKFNPFFYMIDGFRF
                     GFFGKGDVSPWLSLIVVIGFLLGLAWVCLKMLKSGYKLRG"
     gene            12079..12324
                     /locus_tag="PKL42_01330"
     CDS             12079..12324
                     /locus_tag="PKL42_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HNU65739.1"
                     /translation="MLSAAQLESYIKQGLPCNYIQVLGDDGTHFEAVVVSPLFTGKNM
                     VQQHQLVYGALGDRMRAEIHALSMKTYTPDQWQALSR"
     gene            12355..12666
                     /gene="grxD"
                     /locus_tag="PKL42_01335"
     CDS             12355..12666
                     /gene="grxD"
                     /locus_tag="PKL42_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HNU65740.1"
                     /translation="MDTQAKIKETVTTNPVVLFMKGSPKFPQCGFSNLATQILDACQA
                     KYLAIDVLQDQAIREGIKQFASWPTIPQLYVNGEFVGGSDIMRAMYESGELQTLLNAT
                     K"
     gene            12667..13929
                     /gene="murA"
                     /locus_tag="PKL42_01340"
     CDS             12667..13929
                     /gene="murA"
                     /locus_tag="PKL42_01340"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNU65741.1"
                     /translation="MDKLIIQGGNRLHGEVTVSGAKNAALPILCASLLAETPLQLSGV
                     AALKDIDTTIKLLSMMGVKITRNVDAVTLDASEVASFEATYEMVKTMRASILVLGPLL
                     ARFGTARVSLPGGCAIGSRPVDLHIKGLQAMGAAIHITHGYIQASTLHLPNRRLQGAR
                     YYMDLVTVTGTENLMMAASLAEGTTVLENAAKEPEVVDLAEMLIKMGANITGAGTDVI
                     TIVGVEKLKGTTHHVVCDRIEAGTYMVAAAMTGGEVKLLNARADLLDAVIEKLREAGA
                     TVTHDATSITVKSDGKLKAVNIRTAPHPAFPTDMQAQFMALNTVAEGVAKVTETIFEN
                     RFMHVQEMQRMGADISIDGNTALVKGVEYLDGATVMATDLRASASLVLAGLVARGETV
                     IERIYHLDRGYEHLEAKLNALGANVKRV"
     gene            13934..14581
                     /gene="hisG"
                     /locus_tag="PKL42_01345"
     CDS             13934..14581
                     /gene="hisG"
                     /locus_tag="PKL42_01345"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNU65742.1"
                     /translation="MITIALSKGRIFEETTPLLAAAGIHPAEDPEASRKLIIGTNRED
                     VRLIIVRASDVPTYVQYGAADLGIAGKDVLDEHGGEGLYQPIDLQIAKCKMMVAVRED
                     FDYFASIRSGARLKVVTKYVKTAREHFAAKGMHVDLIKLYGSMELGPLVGLADVIVDL
                     VSTGGTLRANHLKAVEEVGEVSSRLVVNQASFKLNRAKIQPMMDAFTQAIAKRSA"
     gene            14823..16121
                     /gene="hisD"
                     /locus_tag="PKL42_01350"
     CDS             14823..16121
                     /gene="hisD"
                     /locus_tag="PKL42_01350"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNU65743.1"
                     /translation="MNIRKLSTKDAGFEADLKALLAFETAQDDSIDVVVANILKDVKA
                     RGDEAVLEYTNRFDKTSATKLSELEISQAELVAALNGLPTAQREALQSAAARVRSYHE
                     KQLMQSWSYTEADGTLLGQQVTSLDRVGLYVPGGKAAYPSSVLMNAIPAKVAGVQELI
                     MVVPTPNGEKNALVLAAAAVCGVDRVFCIGGAQAVGALAYGTQTVPQVDKIVGPGNAY
                     VAAAKRRVFGVVGIDMVAGPSEILVICDGKTNPDWIAMDLFSQAEHDELAQSILLSPD
                     AAFLTEVAASMQRLVEEMPRKEIINTSITDRGALILTKDLDEAVAICNYIAPEHLELS
                     VEDPLTLAKKIKHAGAIFMGRDTCEALGDYCAGPNHVLPTSRTARFSSPLGVYDFQKR
                     SSLIMVSSEGAQVLGKVAATLAYGEGLQAHARSAEYRLKK"
     gene            16118..17176
                     /gene="hisC"
                     /locus_tag="PKL42_01355"
     CDS             16118..17176
                     /gene="hisC"
                     /locus_tag="PKL42_01355"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNU65744.1"
                     /translation="MSKFWSDIVHTLTPYVPGEQPKLKNLIKLNTNENPYPPSPKVLD
                     ALKAEAAETLRLYPDPNSDKLKVAIASYYGLQANQVFVGNGSDEILAHVFQALLKHDK
                     PLLFPDITYSFYPVYCGLYQIQYKTVALNHQFEIDVEDYLQPNGGIILPNPNAPTGIP
                     LPLAKIEQLLQRNPESVVVVDEAYVDFGTESAVKLIQQYPNLLVTQTLSKSRSLAGLR
                     VGFALGHPDLIEALIRVKDSFNSYPIDRFAEAGAIAAMQDQAYFEHTCQQVVATREQL
                     VKDLQGLGFDVLPSGANFIFAKHRVKAGSELSGQLREKGIIVRQFQSPARIKEYLRIT
                     IGTEVQSRALIDALKQLI"
     gene            17429..18427
                     /gene="hxlA"
                     /locus_tag="PKL42_01360"
     CDS             17429..18427
                     /gene="hxlA"
                     /locus_tag="PKL42_01360"
                     /EC_number="4.1.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043801 - hexulose-6-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hexulose-6-phosphate synthase"
                     /protein_id="HNU65745.1"
                     /translation="MAQTQMALDSLDFDGTVALATKVAPHVDILEIGTPCIKHNGIKL
                     LEVLRAKFPNNKILVDLKTMDAGFYEAEPFFKAGADIVTVLGTADIGTIKGVIDVANK
                     YGKQAQVDLINVADKKARTLEVAKLGAHIIGVHTGLDQQAAGQTPFGDLALVTGLKTG
                     AKVSVAGGIKAATVRQVVDAGADIVVAGAAIYGAADPAASAKEITGLAHGNNAGAAAG
                     GFNFLPWVIAIAAAILAFFLYNNLNKPADTAVDGAAVEQQVEGAVDATTEAAGVAVEA
                     TTDAATTAVEATTEAAGAAVEATTDAAAAVEAAGTAVEAKTDGLVGAAKEATGQAE"
     gene            18576..19163
                     /gene="hisB"
                     /locus_tag="PKL42_01365"
     CDS             18576..19163
                     /gene="hisB"
                     /locus_tag="PKL42_01365"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HNU65746.1"
                     /translation="MRNAEIIRNTLETQITVSVNLDGTGVSHFNTGLGFLDHMLDQIA
                     RHGMMDISVIAKGDLHIDAHHTVEDIGITFGQAFAKAIGDKKGIRRYGHAYVPLDEAL
                     SRVVLDISGRPGLEFACTFTRATIGEFDVDLIHEFFQGFVNHANITLHIDNLKGHNAH
                     HQAETIFKAFGRALRVAVESDARMAGIMPSTKGSL"
     gene            19188..19847
                     /gene="hisH"
                     /locus_tag="PKL42_01370"
     CDS             19188..19847
                     /gene="hisH"
                     /locus_tag="PKL42_01370"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNU65747.1"
                     /translation="MRKIDIAVVDYGMGNLRSVSKALEHVAPEKSVLVTSCPDEISKA
                     ERVVFPGQGAMPDCIRELDARGLRESVLFAARNKPFLGICIGLQMLFEHSEEGDAAGL
                     GVFQGQVKRFAAADMHDEHGEKLKVPHMGWNQVYQVVDSENAAHALWQGIESGTRFYY
                     VHSYYVAPADQVITTGYTRYPSEFTSAIAQNNLFAVQFHPEKSATAGLQLLHNFVNWQ
                     P"
     gene            19917..20660
                     /gene="hisA"
                     /locus_tag="PKL42_01375"
     CDS             19917..20660
                     /gene="hisA"
                     /locus_tag="PKL42_01375"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNU65748.1"
                     /translation="MLIIPAIDLKDGHCVRLKQGLMEESTVFSEDPGAMAKHWVDAGG
                     KRLHLVDLNGAFAGKPVNEAAIKSIVAAVKGEIPIQLGGGIRDLETIERYLDNGIDFV
                     IIGTAAVKVPGFLHEACDAFPGQVMVGLDAKDGKVAIDGWSKLTGHDVIDLAKKFEDY
                     GVSSIVYTDIGRDGMLTGVNIEATVALAQALTIPVIASGGVTNLDDVRKLCAVADEGI
                     IGTITGRAIYEGTLDFTAAQKLADELSAR"
     gene            20650..21414
                     /gene="hisF"
                     /locus_tag="PKL42_01380"
     CDS             20650..21414
                     /gene="hisF"
                     /locus_tag="PKL42_01380"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNU65749.1"
                     /translation="MLAKRIIPCLDVTAGRVVKGINFLELRDAGDPVEVAKRYDEQGA
                     DELTFLDITASSDNRGLLLHIIEEVAAQVFIPLTVGGGVREVEDVRRLLNAGADKVSI
                     NTTAVLNPKMVQDAAARFGSQCIVVAIDAKKVENQPFEWEVFTHGGRKATGLDAVKWA
                     EHMVSLGAGELLITSMDRDGTKIGFNNPLNRAISNAVDVPIIASGGAGSLQHLVEGIK
                     LGGADAVLAASIFHYGEYTIQEAKEFMQKNGIEMRL"
     gene            21411..21812
                     /gene="hisI"
                     /locus_tag="PKL42_01385"
     CDS             21411..21812
                     /gene="hisI"
                     /locus_tag="PKL42_01385"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HNU65750.1"
                     /translation="MTSTSELWLDKVHWDANGLVPVIAQEYDTGRVLMFAWMNREAFQ
                     RTNETQQAVYWSRSRNRLWHKGEESGHIQKIHEIRLDCDEDVVLIKVEQVGGIACHTG
                     RHNCFFQKLENNQWVTDQPVIKNPNEIYNHE"
     gene            21805..22122
                     /locus_tag="PKL42_01390"
     CDS             21805..22122
                     /locus_tag="PKL42_01390"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HNU65751.1"
                     /translation="MNDVLNRLSELLEQRKSADPATSYVAKLYAKGMDSILKKVGEEA
                     TEVVIAAKGGNQQEIIYETADLWFHTLVMLAKADINPQEVLDELARREGLSGITEKEN
                     RTK"
     gene            22119..22457
                     /locus_tag="PKL42_01395"
     CDS             22119..22457
                     /locus_tag="PKL42_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNU65752.1"
                     /translation="MSADCIFCKIVSGAIPSKKIYEDDDFVAFHDINPSAKVHFLIVP
                     KLHIESLQTCDASHQLLLGKALLLAPKLAAEQGLKGFKTLINTGREGGQEVFHLHVHV
                     MGGWATIPKL"
     gene            22468..22677
                     /gene="tatA"
                     /locus_tag="PKL42_01400"
     CDS             22468..22677
                     /gene="tatA"
                     /locus_tag="PKL42_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HNU65753.1"
                     /translation="MGSFSVWHWLIVLLIMVLVFGTKKLKNLGGDVGGAVNEFKKAMK
                     DGQVTEETDKNGTTIEGEVTEKTKQ"
     gene            22701..23174
                     /gene="tatB"
                     /locus_tag="PKL42_01405"
     CDS             22701..23174
                     /gene="tatB"
                     /locus_tag="PKL42_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HNU65754.1"
                     /translation="MFDISFSELIVVAVVALLVIGPEKLPKVARTLGALTGRMQKYVA
                     QIKDEVNREVRFQELQQLQQEIQTAAFNTKEAIQSSLNDVKAQVSVEAEAFQLTALDP
                     TPPAKPATQPQTKPVKPKATQSGTAELPLKACKGQSAVNKTTAEKKRVAKAAKEA"
     gene            23183..23926
                     /gene="tatC"
                     /locus_tag="PKL42_01410"
     CDS             23183..23926
                     /gene="tatC"
                     /locus_tag="PKL42_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNU65755.1"
                     /translation="MTPTDNFISHLIELRNRLLRIVVGFVVIFVVFFPFANDIYALLA
                     APLLGKLPAGGQMIATAVTTPFFVPMKVAMMAAFMVSLPHTVYQIWAFVAPGLYAHEK
                     KFMIPVIIASCLLFLLGMAFAYFLVFPVVFGFIVGTAPIGVAVMTDIDNYLDFVLTLF
                     FAFGLAFQVPIAVVMMVRFGWVTIAQLKEARGYVIVGAFVIGAIFTPPDIISQFMLAV
                     PMWLLYELGILIAALQKKPPEPSIRPPAE"
     gene            complement(24216..25409)
                     /locus_tag="PKL42_01415"
     CDS             complement(24216..25409)
                     /locus_tag="PKL42_01415"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HNU65756.1"
                     /translation="MNHSLSKKLWLTFAQSVTVCLGLAFVLRMFFPDLLVTLFDASAL
                     KKNEVVVKQVEPQPHATTAGSYRDAVQKAAPAVVNIFTSKKVAANPQQRFLDDPLFRH
                     FFGDQFDDEESAAQPENSLGSGVIVTEQGLILTNNHVVATADEIEVALSDGRKLSAKV
                     VGTDPDTDLALIKIEAEHLPAITFAKSDQLNVGDVVLAIGNPFGVGQTVTQGIVSALG
                     RNHLGINTFENFIQTDASINPGNSGGALIDVQGNLVGINSAIYSRSGGSMGIGFAIPA
                     TLARQIMDQIVAQGNVTRGWIGIEAQDLTPELAESFKLKTAEGALVAGVLPNSPADKA
                     GLRAGDILLSIDGKPITDTSSMLNLIAALPPFKNATVKIARDENMLDIVILIGKRPKP
                     VALKE"
     gene            25513..26262
                     /locus_tag="PKL42_01420"
     CDS             25513..26262
                     /locus_tag="PKL42_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNU65757.1"
                     /translation="MVKMSELTHYTQQLLQVERFKDYCPNGLQVEGKAEVRKIISGVT
                     ASMALLEAAHQAHADLVLVHHGYFWRGEDARVVGIKRKRLAFLLQHDINLLAYHLPLD
                     AHPELGNNVQLGQVLGFPLAGFAGETNMISHATLTHTTTLRALSAHIESTLQRAPLVI
                     GDPEKLVQKVAWCTGAAQGYIEDAIALGVDAFISGEISEQTTHQALESGVSYISAGHH
                     VTERYGVQALGQHLADKFGITHEFIDIGNPV"
     gene            26429..27046
                     /gene="petA"
                     /locus_tag="PKL42_01425"
     CDS             26429..27046
                     /gene="petA"
                     /locus_tag="PKL42_01425"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HNU65758.1"
                     /translation="MSANQIDKNLKQDVNERSAVDQEKRKFLIATSAVGAAGAAVVAV
                     PFVASMTPSERAKAAGAPVEVDVSKIAPGEMMVSEYRGQPLWVIHRTDEQIAKLSNHN
                     AQLTDPDLEVPQQPEYCKNKERAINPKYAVMLGTCTHLGCSPAQDFLTTADWDGGFIC
                     ACHGSKFDLAGRVFKGSPAPTNLVIPKHQYLTETTLLVGEDKESA"
     gene            27046..28329
                     /locus_tag="PKL42_01430"
     CDS             27046..28329
                     /locus_tag="PKL42_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HNU65759.1"
                     /translation="MSLFTKKADELLGWVDARFPLTSNIKAHLTEYYAPKNFNFWYFF
                     GSLALLVLVLQIVTGIFLTMHYKPEALMAFASVEYIMRDVPWGWLIRYMHSTGASLFF
                     VVIYLHMFRGLLYGSYRNPRELIWLFGVGIFLALMGEAFFGYLLPWGQMSYWGAQVIT
                     SLFGTVPFIGPDIAEWLRGDYLTSDATLNRFFAFHVIALPLVILGLVAAHILALHEVG
                     SNNPDGVEIKKLKDKKTGIPLDGIPFHPYYTVKDMVGVVVFLIVFAAIIFFAPEMGGY
                     FLEANNFVPADPLVTPAHIAPTWYFTPYYSILRAVPPIGISQFPGVAAMGLAVLAFAF
                     LPWLDRSPVKSIRYKGNLYKGWLAAFVVSFLVLGYLGTVPANVWGQFGAWLGKADIAT
                     VVARIFTVVYFAFFILMPWYTKKDKTKPVPERVTG"
     gene            28335..>28781
                     /locus_tag="PKL42_01435"
     CDS             28335..>28781
                     /locus_tag="PKL42_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HNU65760.1"
                     /translation="MKIQMIKKAIYVLAIVPLLAFANVEVHLDKAPIDPSDQASLQRG
                     ARTFVNNCLNCHSANYMRYNRLQDIGLTEKQIKENLLFTAEKVGDPMTSAMNKKDAKK
                     WFGVAPPDLSVEVRARGADWVYTYMRSFYRDETRPSGWNNVVFDKVA"
BASE COUNT         7966 a         5750 c         7093 g         7972 t
ORIGIN      
        1 ccaagcctgc tttggctacg ctatagacga tgtagccctt tttgggcctt tcgacatgca
       61 tgtcagtaat atttacaatg cagccatggt tttttcgtaa ctcaggtgtg gcggcctgcg
      121 ataaaaataa aggcgccttt aaattactac ccatgaggtc atgccacttg gcttcatcaa
      181 tgtggccgat ttcgctggga taataggtcg aggcgttgtt gatgagtacg tcaagccggc
      241 caaaatgatg gatggtttgt ccaataagat gcggtaaatt gacgatgtcc aataaatcag
      301 cctgaatgat ggcaacagaa ttggcgcgtt gtaaattcag ctcggcttgc agtgcgcgcg
      361 cttcactggt agaactacga taatgaatca tcaggtttgc accctgagcg tgtagttggc
      421 ggcatattgc cgcgcccacg cgtttggcgc cgcccgtaat gagcactact ttatttatgt
      481 tgtttgcaat catgcaatat ccaattctaa ttgttcgcta ttataatcca agaccatgag
      541 cacattacct aaaccagccc ctgaggccga aaaacatagc gagcagcttg tcttaaagat
      601 tcaggatgaa atccaaagcg ctggaggctg gctggatttt gcgcgattta tgcagttagc
      661 gttatatgcg actggcttag gatattacag cgcaggcagt caaaagttcg gagatattaa
      721 aaaaggtggc ggagattttg tgaccgcacc acaaatttct ccgctatttg cgcaaacgct
      781 tgcaaatcaa atgatacaag tcctgcaatt aacgcaaggc agtgtgctag aacttggtgc
      841 aggcactggt aagctggcgg cggactgttt acttacattg gcgaaattaa atggcttgcc
      901 cgaacgctat tttattttgg aagtaagtga ccacttgcgg caggtgcagc ttgaaacttt
      961 atcgcaaaca ttgccgcaag atgtgcttca acgggtcgaa tggctaaacg aattgccgca
     1021 gcagtttgca ggcattgtga ttggtaacga ggtgctagat gcaattccag taaacatcgt
     1081 acataaacat gccaatggca ttgaagtgtg cggcattgcc gtagtcaacc acaagcttgt
     1141 ttggcaaaac cgacccatca atcaacacgg caccccaagt gaaaaaagcc tatttgaagc
     1201 agtgacaaac ctcaatttac ccgagggtta tacgacagag atttgccctg ccgccagtgg
     1261 gttaatgact tcactcgcca atatgttaaa aaacggcgca atattgatga ttgattacgg
     1321 cttttcagca cgtgaatatt atcatccgca gcgcaatcag ggtacgctga tgtgccatta
     1381 tcagcactat gcgcacactg acccgttaat ttatgtgggg ctacaagata tcacggccca
     1441 cgtagatttc actagcattg cccatgctgc agtgaagggt ggcctaagtt taagtggctt
     1501 ttgtagtcag gcgcagtttc tgatgaattg cggcattttg acgctgttgt ctgaagtttc
     1561 cccaactgat atggcacgct atgccccgtt ggcggccgcg gctcaaaagc tgttatctcc
     1621 tgctgagatg ggcgatttat ttaaagtgat tgcttttacc aaagagatag atacgccgct
     1681 gatgggattt ttgactgggg ataaatcgca caccttatag cgcttaatga ttcaaaatat
     1741 agccattaaa agtataatgg cggctatgac aaacgatatc aaaactgaac atcaatccct
     1801 gcaagaaatc cctaaagacg aaaaaagtac gaatgacgaa ttaaaacatc gccgcattcg
     1861 cagttttgta ttgcgccaag ggcgtgtgac aaaagggcag gagcgcgcac tagaaattgg
     1921 ttggccaaaa tttggtattc aatatgcagc caagccaatt gatttgaata cggtgtttca
     1981 acgacaagca tcagctaaaa ttctagaaat tggttttgga atgggagaaa caactgcaaa
     2041 aattgcgcaa acgctacccc attgtgattt tttagccgca gaagtacata cgccaggcgt
     2101 tggcgcattg ttaaagctta ttgaagcgca tgcactgacc aatattcgca ttattcagca
     2161 tgatgtggtt gaagtgctgc aaaacatgct ggcagacgct agcctcgatg gcgtacatat
     2221 tttcttccct gacccatggc acaaaaaacg ccatcataag cgccggttaa ttcaggtaga
     2281 gtttgtaaag ctgctttgta ctaaacttaa agtgggcggc tatttgcatg tggcaaccga
     2341 ttggcaagaa tacgctgagt gggtgttagc cgtgctgagt gcggaaacac agcttaccaa
     2401 taccgcagaa aattatgcgc ctaaaccgag ctaccgcccg ctgactaaat ttgagaatcg
     2461 cggacttaaa ttagggcacg gtgtttggga tatggtcttc accagagttt gagtcttcat
     2521 caaatagcca tttgccaatc actttttctg cttcttccac gccaagtttt ttcaagctag
     2581 aaaatagctg aatgctgcac gcgttacccc aagtttctac taactctttg cgaactttgt
     2641 tgagtgtcag tgaggcttct ccacgtgata atttgtcaga cttagtgagt aaaacatgta
     2701 ccggtttacc acttgggcaa aaccagtcaa gcatttggcg gtctagcggg gtgagtgggt
     2761 ggcggctatc catgaccagt accaagccac ctaagctaga gcgctcactg aggtaacgtg
     2821 ccaatacatt ttgccagtgc gcgcgcatcg cctcaggcac ttttgcgtac ccatagcctg
     2881 gtaaatcaac caaatggcga tcattgccaa gggtaaaaaa gttaatgagc tgtgtgcggc
     2941 ctggctgttt actcacaaaa gcgaggcgat tatgattggc taacgtgttc aatgcgctgg
     3001 attttcctgc gtttgagcgc ccggcaaacg ccacttcaat gccgcttgca ggcggtaagt
     3061 cgcgtaagtg atgcgcggag ataaaaaaag tggcgttttg gaacaatggc atttaatttc
     3121 aattcttgag tgataagtgt tgctttgaaa cctcaaacgt gaggcgcagt taataactgt
     3181 gaaaaatgct atcacgaaag ggtcttttta gctaaaatgt tgagtaattc tttgaataac
     3241 ctaagttttg ggagctgtaa tgcaatttcg tcatcgggta gggttaaatt cagtagctgc
     3301 aagtttaacg ggtttaatgt tgagccttgc ttttagtttg cctgcgcaag cggcagatgc
     3361 ggtcaattcg gctgaagaaa ccgctaaaaa tgtctgttcg gcttgccacg gagtcgatgg
     3421 caacagcgcg attacgctga acccaaaatt ggcgggtcag catcctgaat atctgcaaaa
     3481 gcaactcaca gaatttaaat ctggtaaacg tgccaacgcg gttatggcgg gtattgcagg
     3541 tgcgttaagt gatgaagaca tgagaaactt agcggcctat tattcaaaac aaagtttaaa
     3601 gctcgggcaa gcagctagta atggcaaagg ttctttgggt gaaaaaatct atcgtggtgg
     3661 tgttgctgcc accaatgtgc cagcctgtgc aagttgccat ggggcaaacg gtgcgggtat
     3721 tcctaagcag ttcccgcgcg taaacggcca acatacagat tacacattgg cgcaactcaa
     3781 agcgtttcgc tcaggtgaac gcgcaaatgc accaatgatg atggtcattt ctgccaaaat
     3841 gacagatgca gaaatggcgg cagtgtctga ttatattcaa gggttacgct agatttttac
     3901 gcaaacgtaa tgcgtttaaa ttgagagagt cttggagtcc ataaagattt atttagggta
     3961 gcggaaacgt taccctaaat gctttttaat gccctgtttt aacaccaacc ttttgcgatg
     4021 ctctgaaagc cttataaata aaggctcgca gggcattgtt tttctctcga aggatttagt
     4081 ggttgttttt aagtcaattt aacccatcta aatagcttgt tcaggggcta acatttccag
     4141 cctaaagcgc acttcaccat tcacattatc gaccaaactt aattcatagc cagcctgact
     4201 cgcctgctgc gctgcgtgat acaaccccac acctaaacca ttttttgaag tgacatgtga
     4261 tttaaataac tgctctgcaa tgtcttgtgg catggcggag ccagtatcgg tgacttcaag
     4321 gcaaaagtgg ttggatggca ttaaggtcac ttttatttgc gtgttggctt ctaatttggc
     4381 tttttcaagc gcgttttgta ctaagttatc caccacgcta tcgagcacgt ctgggtcaat
     4441 gtcggctttt ggtaaaacag cgggcgattc aaactgaatt tgatggttgc tattgagttg
     4501 cctgaaactt ttccaccaag tggccacttt tgcctgacgt tttttctcaa cacttggccg
     4561 ctcgagcttg gtgagcgtgg cggcaaggcg ctgattgagt cttggtaatt gttttttaat
     4621 caattcaatc aatctaatgt caccttcagc gtcactcgtt tgttcggcgg cagaagttaa
     4681 cgtgcctaaa gactgcagaa tatttttaat atcatgcgtg aggcgtgagc ctgtttcata
     4741 aaacgtttgc atataggtat tttggctgat cgtttcttca cggcatttgg cctcatagaa
     4801 ttcgctcatg atttgcgtga gtagttgtac gtgtaaatac atggcagggc taaactgcca
     4861 gcgtgaataa agtgtgagat gcaattgctg aaagctaaag gtggagatgt gcggtgtagc
     4921 aaaaccagct tgatgtttcg cgccatccac ctcccaaagt acaccgctca cccattttaa
     4981 agcggctagc tcgcgcatag aggcttctaa aaaatcactg gcggttaatt cataatcagc
     5041 atgcatggcg atttttctta accattcttc aaagggcagg cctagactta ataagtagcg
     5101 tgacatgagc aactctaagc ccgaaaaagt ctcgctaggg ttccataacc agctgagcat
     5161 tactaaggtc acagccaagc ccagcaccac ataaatcagc agttttacgt aatgcacttg
     5221 ccaagtcacg ccaatcgcaa agccgcctaa aataataatc agcgtcacta aataaatcag
     5281 cagcgtatag aaaaagtcga tattctgcgc tggcgaaacg ggcttatttt ttgctgatag
     5341 aaacaaaatg gtcaatggca gaataggcag taaataagtc agtaagaatt cagcggcgat
     5401 taaatcatct tttgcattga gcagttttgg caccacccaa agtaatagca cggcaagtaa
     5461 ataagttgcc gctaaaatat taggtaatct ggcacgcgtt gtttcgcctg aaataatgcg
     5521 tccgccaatc agtgcaaata gaccggcaat ccaaaacgcg gtaatccacc aattcatata
     5581 atacaaggca aaggtggcgc caattaaaac gactaaaatg gatgaccagg agagtttttg
     5641 gttgtgatga aatataggtt gccacaacaa gaagaaaccg aagtggcata aaaataaagc
     5701 gctaaagaat tgactgaaat ctccccaaat caaaagcgca tggagagaaa gtaacatgag
     5761 cgccatcagg cgatcggtat catcaaacat gcgacggtaa attttagaaa gactaagcat
     5821 aaatttttgg ctcatcaatt attgaagttg gcgatatgtt cacaacgacc tagcgtgtta
     5881 ttgtaatagc attcattaca aattaaccat actggacgat gttaaaattt agacacaatc
     5941 gtcaaattat caacacaggc ggcagagttt tctatggaaa aaagcaaaaa aacagataga
     6001 atgttaaaaa ttatcaagaa aagcgtcagt aaatgataaa aacacagctg gcatgaagtt
     6061 tgcatgagga ctgctggcag taaaacagat gcatcatcta caacattaaa aacagtttta
     6121 cttacataat taacgggttt ggaaaattat atgaaaaaac aacaaacagg ttttactttg
     6181 attgagctgg cgattgtgct ggtgattatc ggcttgttgc taggtggtgt gcttaaaggg
     6241 caggagctga ttaatagtgc taaagtaaag aacatggcaa gtgatttgaa gaatattcaa
     6301 atatatgtgt actcatatca ggataaattt aaagcgattc ctggagatga taagtctgca
     6361 ataacgcatg ttggtgctgc tgttaacggt gatggtgatg gggaaattga cggtgcttgg
     6421 aatgcgacaa gtgctaccga aagttattat ttctgggagc atgtgcgtcg tgcaggcatt
     6481 gcatctggct ctaccgattc cgctactact aacaccacct atttgccaac gaataacgaa
     6541 ggtggccctg taggtgtaca atctaaacca caaaacacga ttactggttt gagcgggtcg
     6601 tatgccgttt gttccggtgg tatcgttggt aaaattgcaa aacaactaga ttcacaaatt
     6661 gatgatgggg ataccacaaa aggatccgtt atggttgctg caactgttac tggcaccaca
     6721 gccgcatcgc ctgtggcaac cactgcaact gattttgatt ccactaaata cgtactttgt
     6781 ctagcatttt aataaacaat tagctttgat aaaaaagccc gcaatcgcgg gcttttttgt
     6841 tggctaacag cttatagatg cttaacttac aaacaaatca actttagcgg cagctaattt
     6901 aagcgcttgc ttagtctcca aattgtttta gaatagtggc ttcgtaaaaa gtcgtgctta
     6961 aaatcagaaa aaacaacact gcaacatgac caataatatt gtagagattg ataatttatc
     7021 ctttgggtac aaaggacgtt tgctgcataa aggcatcaat atggtgttcc cgcgtcgcaa
     7081 agtggtggca attatgggcg gtagtggcag tggtaaaacc accttgttgc ggttaattgg
     7141 cggacaaatt aaaccaacca aaggcgaagt gcgcgttgaa ggccaggttg tgcaccagca
     7201 agatcgtgac ggaattttta aattacgtcg tgaaatgggc atgttgtttc agcacggtgc
     7261 tttattcacc gatttaagcg tgtttgataa cgttgcgttt ccgatgcggg aacttaccga
     7321 cttgcctgag tcaatgattc gcgacttggt attaatgaaa ctcaatgcgg taggtttgcg
     7381 cggggcgcac acattaacgc cacctgagct ttctggaggc atggcgcggc gcgtggcctt
     7441 ggcgcgcgca attgcgttag acccaaatat tattatgtac gacgaaccct ttgcaggctt
     7501 agaccccatt tcaatgggtg tgatttgtga cttaattcga agcctcaatg acgcgctagg
     7561 cgcgacttca attatcgttt cgcacgacgt aaaagaaact tttttgattg ctgattatgt
     7621 ttattttgtt gccaatgggg aagtggcggc tgaaggcacg ccgcatgatt tgatgcaatc
     7681 aacactgcct tttgtacatc aatttgtcca tggtgaaaaa gatggcccag taccatttca
     7741 ctatgcggct cctgattatc aaaaggagct aatgcaaaat gtctaaaatt aatttagtca
     7801 ataaattact cagtgggctt cggatgacgg gtcatcgagt aattgagcaa atctggcgtc
     7861 ttggtgctgg cgcaagatta ttctttttaa ccctgacgta ctccgcagaa agctttcgtc
     7921 gtccacattt gattgtgcgc gaaatgtatt tcaccggggt gatgtcactg attattattt
     7981 tagtctctgc tttttttgta gggatggtac ttgcattgca gggttacaat actttgcaaa
     8041 aatacgggtc ttctgaatcc attggtgtgc tggtggcact tgctttaata cgagagctgg
     8101 gtccagttgt aacagcttta ttgtttgcgg gccgggcggg taccgcaatt actgccgaaa
     8161 ttggtttaat gaaagcaacc gagcaacttt cagccatgga aatgatggcc gtcaatccaa
     8221 tttctcgtgt aattgcaccc cgtttttggg cgggcgtgtt ttcaatgcct atattggttt
     8281 gtttattttc aatggtgggc gtattgggcg ggcatttggt cgcggtcgca caaattggtg
     8341 tggatgttgg tgctttttgg tcgcaaatgc aggcgaatgt agaatttgca gatgtcacca
     8401 acatgttgat aaaaagtttt gtgtttggtg tggcatgtac gattattgcc ttatttgaag
     8461 ggtacgatgc accgccaacg gctgaaggcg tcagccacgc gaccactcgt acggtggtga
     8521 cctcagcgct tgctgtgctg ggtttagatt tcatactcac ttcatttatg atagtggcgt
     8581 aaaaaatatt atggctgaat gtggtggcaa ttaacaatag aaaagttggg gaataaacat
     8641 gagtagaaca acaatagatt tatgggtggg tgtgttggtg gttttaggca tcgcggcttt
     8701 ctttgggtta gcgatgaaag tgggaaattt aacgacaagc agtttaggtg aaacctattc
     8761 agtgaccgcc gaatttgaaa atattggtgg ccttaaacca cgagcaccag ttaaaagcgc
     8821 gggtgttgtc gttggtcgcg tagatagcat tacctttaac agtaaaaatt atgcggctga
     8881 ggtgacaatt aaaattgacc agcgttatcc ttttccaaca gatacttttg ctaacattta
     8941 tacagcgggt ctgctgggtg agcaatacat cggcttggaa gctggcggag atgatgtaga
     9001 tctaaaaaat ggtgataaaa ttgccaaaac gcaagatgca attgtgctag aaaaaatgat
     9061 tagtcagttt ttatttagca aggcctcaga aacagaagat aaaaattcca aaaatgaaaa
     9121 agatgcggtc agtgactctg gtgaaaacgc gaagtcaact gaattaggtg acccgccgtt
     9181 ttaggtcagg tgatagttta gtaaacgcgg attgaacact ttcaattttg aatggacaaa
     9241 ggattcagat gaaaatatta cttaaatgga tgattggcgc aagtttactt gcaaatttat
     9301 tagttgccag cgctgcgaat gcagaattgg cgccggatgt attggtaaaa caaactgctg
     9361 atgatgtatt aaatattatc aaaaatgata aagaaattca ggctggtaat cagcaaaaga
     9421 tctacgcact tgctgaagag aaaattttgc caaactttga ctttgatcgc gtaagtcgca
     9481 tggtgctagg caagagttgg agggcagcaa cacctgagca gcaggcaagc tttcaaaaag
     9541 agtttagaag tttgttgcta cgtacatact cggtggcgct tggtaaatat cgtgatcaaa
     9601 ccattgaata taaacctatg cgtgcagagc ctgatgcaaa aaatgttacc gttaaaacgc
     9661 agattttgca aaacggtggc cagcctattt ctgtagatta cagtttggta aaaggggcaa
     9721 atggctggaa agtctatgat attgtgattg aaagtgtgag cttggttact aattatcgca
     9781 gccaattttc gcaagagatt cgtcaaaatg gcattgatag tttaaataaa aaactggctg
     9841 aaaaaaataa atcggccggc gtttagcatt taaggcaata gcataaatgg aaaattcgac
     9901 tttatcatct atcacgcagc acgcaaacac atggagtgtt gcagggccaa tggttgttga
     9961 tacggtgaat aagctattgc ttgagagtgc caaatttaca aataatgcgt cggcgtttga
    10021 ggttaacttt tctaaagtaa cagatgtcga tactgcagcg ttaagtttga tgatggaatg
    10081 gcagcgccgt gcagttgcat tgaacaaaaa agtcgtattt acacatttgc ccgccaattt
    10141 aacgagcttg gcctcacttt atggggtcac tgaatttatt cctatggtgg cttcttgatt
    10201 ttttttaaac tgcaaaatag ctaacaattg caatcgaaaa cagtttgttg taaatcttta
    10261 cggcttgtta aataacaagc cgtattttat tcagtgttgt atccaatagt aacgttttaa
    10321 ttatttatca ctaactgtaa gccaaaagag aatgaagcca gcaatccaat ttaatcgcgt
    10381 acataaaaat tttggcgaat tgcaagcgct gaacggtatt gatttaacca ttccgcaagg
    10441 cgagtttttt ggcttgcttg gcccaaatgg tgctggaaaa tcaactttaa tcaatatttt
    10501 ggctgggctg ctgaagccta cttatggaac agcttctgtc atggggcatg atgtggtgag
    10561 tgactatcag gcagcacgca tggcgcttgg cgttgtgccg caagagttgg tgtttgatcc
    10621 cttttttaat gtacgggaaa tgcttcgttt tcaggcaggt tattttggac gaggtcgcga
    10681 aaatgatgct tgggtggatg aaattcttga aggtctggga ttaacagata aagcgcatac
    10741 caatatgcgt aaactttcag gcggtatgaa gcggcgtgcg ctgattgcac aagccctagc
    10801 acataagcca ccggtaattg tgttggatga gccgacagcg ggtgttgatg tggagttgcg
    10861 gcaaatgctg tgggagttta tcaaaaaact caatcgcgaa ggacatacca tcgtgttgac
    10921 gacacattat ctagaagagg ctgaaacgct ctgttcgcgc gtggcgatga tgaagcaggg
    10981 tgaaattgtt gcattggata gcactgcaaa tttactcaat aagtttgcag gtaaaaattt
    11041 acgtcttgtt ttaggtaacg cgccactacc cgcaccttta ttgtcaatgt taaaaagtgg
    11101 cgagcatggg gtttatatgt tggcgctcag ccatttaagc caagtggaat ttgcattaag
    11161 tgaattgcgc aaagcgaatg tgcagattga agacatgcaa ctgattgaac ctgacctaga
    11221 agatgtgttt atgtcgctgg ttggcagtgc aagtcatcgt aacgatgatt taattataca
    11281 aagctagggt gtcatgagtt taatgaatca aaaatggcgc ggaacgtata ctttatttaa
    11341 aaaggaagta ttgcgttttt ggcgcgtggc atttcaaacg gtggcctccc ccgtacttac
    11401 agccttgctg tatttattga tattttcaca tgtgttggcc agccatgtgc aagtgtatga
    11461 aaacgtaccg tataccgctt ttttaatccc tgggcttatc atgatgtcgt tattgcaaaa
    11521 tgcatttgcg aacagttcat caagcctgat tcagtcaaaa gtgatgggca atattgtatt
    11581 tattttactg acaccattaa gttacttgca gttttatatg gcgctcttgg cggccgcgat
    11641 gatacgcggt ctgttcgtgg gttttgtgat ttatttggtg gccctgtttt ttgtagattt
    11701 gccaattgtg catgttgggt ggattttgac gtttgcggtg ttaggtagtg cgctgttggg
    11761 tacgttcggt attattgcgg gcatttgggc ggataagttt gaccaaatgg ccgcttttca
    11821 gaactttgtg attatgccgc tcacgttttt gtcaggggta ttttattcca tccattcatt
    11881 gccaccgttt tggcaagtcg tatcaaaatt caacccattt ttttatatga tagatggctt
    11941 taggtttggc ttttttggca aaggggatgt ttcaccttgg ctaagcttaa ttgttgtcat
    12001 tggtttttta ttgggattag catgggtgtg cctaaaaatg ctaaaatcag gttataaatt
    12061 aagaggctag tgataagcat gttaagcgca gcacaattag aaagctatat taaacaaggt
    12121 ctgccatgta attacattca ggtgctgggg gatgatggta cgcattttga ggcggtggtg
    12181 gtgagcccat tatttactgg taaaaacatg gtgcagcaac atcaactggt ttatggtgct
    12241 ttgggtgacc gcatgcgcgc agaaattcac gcactctcaa tgaaaaccta tacgcctgac
    12301 caatggcaag cgttaagccg ctaaattgca aaattaaatc aatagaaagt gattatggat
    12361 acacaagcaa aaattaaaga aaccgttacg acaaacccgg ttgtgttatt tatgaagggg
    12421 tcgccaaaat ttccacaatg tggattttca aatttagcta cgcaaatatt agatgcctgt
    12481 caggcaaagt atttggcgat tgatgtgctg caagaccagg caatacgtga aggcatcaag
    12541 caatttgcta gctggccaac gattcctcag ctatatgtta atggtgagtt cgtgggtggt
    12601 tcagatatta tgcgcgccat gtatgaaagc ggcgaacttc aaactttact gaatgcgact
    12661 aaataattgg acaagttaat catacaaggt ggcaaccgcc tgcacggtga agtcactgtt
    12721 tctggcgcaa aaaatgctgc cttgcctatt ctatgcgcct ctttactggc tgaaactccg
    12781 cttcaactaa gtggtgtggc tgcattaaaa gacattgata ccaccattaa attgctctct
    12841 atgatgggtg taaaaattac gcgcaatgtt gacgcggtga cgttagatgc atcagaagtg
    12901 gcctcttttg aggcgaccta tgaaatggta aaaaccatgc gcgcttctat tttggtattg
    12961 ggaccattgt tggcgcggtt tggtacagca cgcgtttcat tgccaggtgg ctgtgccatt
    13021 ggttcacgcc ctgtggattt acacataaaa ggcttgcagg cgatgggcgc ggccattcat
    13081 attacgcatg gctatattca ggcaagtaca ttgcatttac caaatcggcg cttgcaaggc
    13141 gcgcgttact acatggattt ggtaacggtg acgggtacag aaaatctgat gatggcagca
    13201 agcttggctg aagggactac cgtactggaa aatgccgcta aagagccaga agtcgtcgat
    13261 ttagccgaga tgttgattaa gatgggtgca aatattaccg gcgccggaac cgatgtgatt
    13321 acgattgttg gcgtagaaaa gcttaaaggc acaacccatc atgtcgtgtg tgaccgcatt
    13381 gaggcaggca cttatatggt ggctgcagca atgacaggtg gtgaagtcaa gttgctgaat
    13441 gcacgcgctg acttgcttga tgccgtcatt gagaaattgc gtgaagcagg ggcaacggtg
    13501 acgcacgatg caacaagtat tacggtaaaa agtgatggca aacttaaagc agtgaatatt
    13561 cgcacagcac cacatccagc ttttcccacc gatatgcaag cgcagtttat ggcgctgaat
    13621 acggtggcgg aaggcgttgc aaaagtgact gaaaccattt ttgaaaaccg ctttatgcac
    13681 gtgcaggaaa tgcaacgtat gggcgctgac atcagcatag atggcaatac cgcactggtg
    13741 aaaggcgttg agtatttaga tggcgcaacg gtgatggcca cagaccttcg cgcttctgcc
    13801 agcctagtat tagccgggtt agtggcgcgt ggtgaaacgg tgattgagcg catttatcac
    13861 ttagaccgcg gttacgaaca tttagaagcc aagctcaacg ccttgggtgc aaacgttaag
    13921 cgagtttaaa gtcatgatta ccatcgcact ctcaaaaggt cgtatttttg aagaaaccac
    13981 gccattgctg gcggcggcag gcattcatcc ggcggaagac cctgaagctt cacgtaaatt
    14041 gattatcggc actaatcgtg aagatgtgcg cctgattatt gtgcgggcga gtgatgtgcc
    14101 aacctatgtg caatacggtg cagccgattt aggcatcgca ggcaaagacg tgttagatga
    14161 acatggcggc gaagggttgt atcagccgat tgatttgcaa attgccaaat gtaaaatgat
    14221 ggtggcagtg cgtgaagatt ttgattactt tgcatcgatt cgtagcggtg cgcgcctaaa
    14281 agtggtaacc aagtatgtaa aaaccgcacg tgagcatttt gctgcaaaag gcatgcatgt
    14341 ggatttaatc aagctttatg gctcaatgga attgggcccg ttggttggtt tggccgatgt
    14401 gattgttgat ttggtgagta caggtggaac tttacgcgcg aatcacctca aagcagtaga
    14461 agaagtcggt gaggtgagtt cgcggttggt ggtcaatcag gcgtcgttca aactcaatcg
    14521 tgccaaaatt cagccaatga tggatgcatt tacacaggcg attgcaaaac gcagcgcata
    14581 agtaaaaatt aagggaattt aattggtatt ttgaggcatg aatttccccg agagaaaaaa
    14641 tatgctctgg aagccttgta aataaaggct cgcagagcat cgaaaaaggt tgtaggtaaa
    14701 attgaccaaa aaagtgttat tttttgcgct ttataacgct ttataaatag acataaaacg
    14761 cccaaaaaat taagcaatca atatattaag caatcgaaat aaagtgaagt agaaaactca
    14821 tgatgaacat tagaaaacta tccaccaaag atgcaggttt tgaagcggat ttaaaagcct
    14881 tgctggcgtt tgaaactgcg caggatgaca gcattgatgt tgttgttgca aatattttaa
    14941 aagatgtgaa agcgcgcggc gatgaagcgg tgctggaata taccaatcgc tttgataaaa
    15001 ccagtgcaac aaagctttct gaacttgaaa tcagtcaggc ggagttagtc gcggcattaa
    15061 acggtttacc tacagcgcag cgcgaagcct tgcagtcagc cgcggcgcgc gtgcgtagtt
    15121 atcatgaaaa acaattgatg caatcatgga gctacacaga ggccgatggc accttgcttg
    15181 ggcaacaagt cacatcgtta gaccgcgttg gcctatatgt gccgggcggt aaggcggcat
    15241 atccttctag cgtgttgatg aatgcgattc cagcaaaagt ggcgggtgtg caagaactca
    15301 ttatggtagt acccacccca aatggtgaaa aaaatgcgct ggttttggct gctgctgcgg
    15361 tatgtggtgt ggatcgcgta ttctgtattg gtggcgcgca agccgttggc gcgctggctt
    15421 atggcacaca aacagtgccg caggtggata aaattgtcgg cccaggcaac gcttatgtcg
    15481 ctgcggctaa acgccgtgta ttcggcgtgg tgggcattga tatggtggca ggcccttctg
    15541 aaattttagt gatttgtgac ggcaaaacca accctgactg gattgcgatg gatttattca
    15601 gccaagccga gcatgacgaa ctggcgcaat ccattttact ttcgcctgat gccgcatttt
    15661 taacagaagt tgctgccagt atgcaaaggt tagtagaaga aatgccgcgt aaagagatta
    15721 ttaatacctc tatcactgac cgtggcgcct tgatcttaac taaagattta gatgaagcgg
    15781 tggcgatttg taattacatc gcgccagagc acttggaact ttctgtggaa gatcctttaa
    15841 cgcttgctaa aaaaattaag cacgctggtg ccatttttat gggacgtgac acttgtgagg
    15901 cgcttggcga ttactgcgca ggcccaaacc acgtgttgcc tacttcgcgt actgcaaggt
    15961 tctcatcccc gctgggtgtg tatgactttc aaaaacgctc tagcctgatt atggtgtctt
    16021 ctgaaggcgc ccaagtgcta ggcaaagtgg ctgctacgct cgcttatggt gaaggcttgc
    16081 aagcgcatgc gcgttcagca gagtatcgtt tgaaaaaatg agtaaattct ggagtgatat
    16141 tgttcatacg ctcaccccgt atgtgcctgg tgagcagcct aagcttaaaa atctgattaa
    16201 gctcaacacc aatgaaaacc cctacccgcc aagcccgaaa gtgcttgatg cattaaaagc
    16261 tgaggcggca gagactttgc gcctgtatcc tgatccgaat tcagataagt taaaggtcgc
    16321 gattgcaagc tattatggcc tgcaagcaaa ccaagtgttt gtcggcaatg gctcggatga
    16381 aatactggcg catgtgtttc aggccttatt aaagcatgat aaaccattgc tttttcctga
    16441 tatcacctat agtttttacc cagtgtattg tgggttatat caaattcagt ataagacagt
    16501 tgcactgaat catcagtttg aaattgacgt agaagattat ttacaaccga acggcggcat
    16561 tattctgcca aacccgaatg cgccaacggg aattccgctg ccgcttgcta aaattgaaca
    16621 attgctccag cgcaaccctg agtctgtagt ggtagtcgac gaggcctatg tggattttgg
    16681 tacggaatca gccgtgaagt tgattcaaca ataccccaat ttattagtca cgcaaacgtt
    16741 atctaaatct cgttcacttg ctggtttgcg cgtgggtttt gcattgggtc acccagattt
    16801 gatcgaagcg ctgattcgcg ttaaagatag ctttaactct tatcccattg atcgttttgc
    16861 cgaagctggt gcgattgcag cgatgcaaga ccaagcgtat tttgaacata cttgccaaca
    16921 agtcgttgcg acacgtgaac agttagtgaa agatttgcaa ggcttaggtt ttgatgtgct
    16981 accttctggc gctaatttca tttttgccaa gcatcgggtg aaagccggca gtgaactctc
    17041 tggccaattg cgagaaaaag gcatcatcgt gcggcaattc cagtcaccag caagaattaa
    17101 agaatatctt cgtatcacca ttggcacaga agtgcagtct cgagcgctaa ttgatgcgct
    17161 caaacagctc atttaaattt tctatattta tttaaaagta gtgcgattgc gactgcttga
    17221 atcaagaaag atttaaaaag cttaactttc atagtcaatc aaaaattatg caggtttcca
    17281 tgaaataaag taaaaaattg tttaatttca tgcgaacagc gtgttattat cgcgaaatgt
    17341 gtgtttttac acaaaaaaac tattttaaaa cgaacttttt taactcaagt aagttacttg
    17401 taatttactt tttgcgggag gatgtagcat ggctcaaacc caaatggcac tagattcatt
    17461 agatttcgac ggtaccgtcg cattagcaac taaagttgct ccacacgtag acattttaga
    17521 aattggtaca ccttgcatca agcacaacgg tatcaaatta ctagaagtat tacgtgctaa
    17581 attccctaac aacaagatct tagttgactt gaaaacaatg gatgctggtt tctacgaagc
    17641 tgagccattc ttcaaagcag gtgctgacat cgtgactgtg cttggtactg cagatatcgg
    17701 tacaatcaaa ggcgtgattg acgttgccaa caaatatggc aaacaagcgc aagttgactt
    17761 gatcaacgtt gccgacaaaa aagcacgtac attagaagtg gctaaattgg gcgcgcacat
    17821 cattggtgtg cacactggtt tagatcaaca agcggccggc caaacaccat ttggcgattt
    17881 agccttagtc actggtttaa aaacaggcgc taaagtttct gttgctggtg gcattaaagc
    17941 tgctacagta cgtcaagtgg ttgacgcagg cgctgacatt gttgttgctg gtgcagcgat
    18001 ttacggcgca gctgaccctg cggcttctgc aaaagaaatc acaggtttgg cacatggcaa
    18061 taacgctggt gctgctgctg gtggttttaa ctttttacca tgggtaattg cgattgctgc
    18121 agcaatcttg gctttcttct tgtacaacaa tttgaataaa cccgctgaca cagcagtaga
    18181 tggtgcggca gttgagcaac aagttgaagg cgctgtagat gcaacgacag aagctgctgg
    18241 tgtagcagtt gaagcgacga cagacgcagc tactacagca gtagaggcta ctactgaagc
    18301 tgctggtgca gcagttgaag cgacgacaga cgcagctgca gcagttgagg ctgctggtac
    18361 agcagtagaa gctaaaacag atggtttagt aggcgcagct aaagaagcga caggacaagc
    18421 tgaataattt taatttagct ttggtctaac taaaagccgg ctcttgagag ccggcttttt
    18481 tgttttagtt atgcaacaaa tcaagtgtaa ccttacgcat aacttcaagt ttgggttaaa
    18541 atgccaatac attttcaata taagtttaat gtcacatgcg caatgctgaa attattcgca
    18601 ataccttaga aacgcaaatt acagtctctg ttaatttaga cggcacaggc gtttctcatt
    18661 tcaatactgg attgggcttt ttagatcata tgctcgatca aattgcccgt cacggcatga
    18721 tggatatcag tgtgattgca aaaggtgatc tccatattga tgctcaccat accgtggaag
    18781 atattgggat tacttttggt caagcctttg ctaaagcaat cggcgataaa aaaggcattc
    18841 gtcgctacgg tcatgcttat gtgccgttag atgaagcgtt gtcacgcgtg gtgttagata
    18901 tttctggtcg gcctgggctg gaatttgcat gtacgttcac gcgcgcaacg attggtgaat
    18961 ttgatgttga tttgattcat gaattctttc aaggattcgt taatcatgcc aatatcacgc
    19021 tgcatattga taacctaaaa ggtcataatg ctcaccatca agctgaaacg atttttaaag
    19081 cctttggccg tgcgctgcgc gtagcagttg agtctgatgc gcgtatggca ggcataatgc
    19141 cgtccacaaa aggcagtctc taagtttaga agatcgctta atttagtatg cgtaaaattg
    19201 atattgcggt cgtagactat ggcatgggca acttacgctc agtgtctaag gcgcttgaac
    19261 acgtggcgcc agagaagtct gtattggtga ctagctgtcc agatgaaatc tcgaaagctg
    19321 aacgtgttgt ctttcctgga caaggcgcga tgccagactg tattcgagaa ctcgacgccc
    19381 gtggcctgcg tgagtcagtc ttatttgcag cgcgcaataa acctttttta gggatttgta
    19441 ttggtctaca aatgctattt gagcattctg aggagggcga tgcagcaggt ttgggcgttt
    19501 ttcaggggca ggtaaagcgc tttgctgcgg ctgatatgca tgatgaacat ggcgaaaaat
    19561 taaaagtacc gcacatgggc tggaatcagg tttaccaagt cgttgacagt gaaaacgcag
    19621 cgcatgcact atggcaaggc attgaaagtg gcacgcgttt ttattatgtg catagttatt
    19681 atgttgctcc cgcagaccaa gtaattacaa caggttatac tcgttatcct agcgagttta
    19741 ccagtgcaat tgcacagaat aatttatttg cagtacaatt tcacccagaa aaaagtgcaa
    19801 ccgctggctt acagttgctg cacaattttg taaactggca gccttaacca aaagactaca
    19861 tcgatttatt tcttattttt tactgttcaa tattcatcat ttttatttaa tttactatgt
    19921 taattatccc agcaattgat cttaaagatg gccactgcgt caggttaaaa caaggtttaa
    19981 tggaagagtc gacggtcttt tcggaagatc cgggtgcgat ggcaaaacat tgggtggatg
    20041 caggaggtaa gcgtctgcat ttggttgact tgaatggtgc ttttgcaggt aaaccggtga
    20101 atgaagcagc cattaaatcc attgtggcgg cggtaaaagg tgaaattcct attcaattgg
    20161 gtggtggtat tcgtgattta gaaacaatag agcgttattt agataacggt attgattttg
    20221 tgattattgg cacagctgca gtgaaagtgc cgggcttttt gcacgaggct tgcgatgctt
    20281 ttcccgggca ggtgatggtt ggtctggatg caaaagacgg taaagtcgcg attgacggtt
    20341 ggtcaaaact cacggggcat gatgtgattg atttagccaa aaagtttgaa gattacggcg
    20401 tgagctcgat tgtttacact gatattggcc gcgatggcat gttgacgggc gtcaacattg
    20461 aggccacagt tgcattggcg caagccttga cgatccccgt gattgcctcg ggcggcgtga
    20521 ccaacttaga cgacgtacgc aagctttgcg cagtggcgga tgaaggcatt attggcacca
    20581 ttacgggccg cgccatttat gaaggtacgc tagattttac agcggcgcaa aaacttgcgg
    20641 atgaactaag tgctcgctaa gcgtatcatt ccttgcttag acgtgacggc gggtcgcgta
    20701 gtgaaaggga ttaacttcct tgaactcaga gacgccggcg atccagttga agttgcaaag
    20761 cgttatgatg aacaaggtgc ggatgaactg acgtttttag atatcacggc aagttctgac
    20821 aaccgaggct tacttctgca tatcattgaa gaggtcgcgg ctcaggtatt tattccgctt
    20881 actgtgggcg gtggtgtacg cgaggttgaa gatgtgcgtc gtttgctcaa cgctggggct
    20941 gataaggtca gcatcaatac cacagcggtg ctgaatccaa aaatggtaca agacgcagcg
    21001 gctcgctttg gctcacaatg catcgtagta gccattgacg cgaaaaaagt tgagaaccaa
    21061 ccgtttgagt gggaagtatt tacgcacggt ggacgcaagg ctacagggct agacgccgtg
    21121 aaatgggctg aacacatggt gagccttggc gcaggtgagt tgctgattac cagtatggat
    21181 cgtgatggga ctaaaatagg ttttaataac cctctcaacc gagcgatttc caatgcggtt
    21241 gatgtgccga ttatcgcttc aggcggtgcg ggtagtctgc aacatttagt ggaaggcatt
    21301 aaattaggcg gtgctgatgc agtgctggcc gccagtattt ttcattacgg agagtacaca
    21361 atccaagaag ccaaagaatt catgcaaaaa aacgggattg agatgcggct atgacaagca
    21421 cttcagaatt atggttagac aaagtgcatt gggatgccaa tggtttggta cccgtgattg
    21481 cgcaagagta tgacacagga cgggtgctca tgtttgcctg gatgaatcgt gaggcctttc
    21541 agcgcacaaa tgaaacacag caggcggtct attggtcacg ctcgcgcaac cgcttatggc
    21601 ataagggcga ggaatctggg catatacaaa agattcatga aatacgccta gattgtgatg
    21661 aggacgtggt actgattaaa gtagagcaag tgggcggcat tgcctgccac acagggcggc
    21721 acaattgttt ttttcaaaag ttagaaaata accaatgggt caccgaccag cccgtcatca
    21781 aaaacccgaa tgagatttat aatcatgaat gatgtgttaa atcgattgtc tgagttgctg
    21841 gagcagcgca aaagtgctga tcccgctacc tcctacgtgg caaagttata cgctaaaggg
    21901 atggacagta ttctcaaaaa agttggcgaa gaggcgactg aagtggtgat tgctgccaaa
    21961 ggcggcaatc aacaagagat tatttatgaa acggccgatt tgtggtttca cacgttagtc
    22021 atgcttgcaa aagcagacat caacccacag gaagtgcttg atgagttggc gcggcgcgag
    22081 ggtttgtcag gaattactga gaaagaaaac cgaacaaaat gagtgcagac tgcatttttt
    22141 gtaaaatagt aagcggtgca attccttcta aaaagattta tgaggatgat gacttcgtgg
    22201 cttttcatga catcaatcca tcggcaaaag tacatttttt aattgtgcca aaattgcata
    22261 tagaaagcct gcaaacctgc gatgcctcac atcagctttt gcttggtaaa gccttgttat
    22321 tagcgccaaa attggcggca gagcaagggc taaaggggtt taaaacgttg attaacaccg
    22381 ggcgtgaagg cggacaagag gtctttcatt tgcacgtgca tgtcatgggt ggttgggcaa
    22441 ccatccctaa attataggag agttatcatg ggttcattca gcgtttggca ttggctcatc
    22501 gtattgttga ttatggtgtt ggtgtttggt actaaaaagc taaaaaattt gggcggcgac
    22561 gttggcggtg cagtcaatga gtttaaaaag gccatgaaag acggccaggt gacggaagaa
    22621 acggataaaa acggcacaac cattgaaggt gaagtcacag aaaagactaa gcagtaaatt
    22681 ttaagtaaca agcgcatatt gtgtttgata tttcattttc agagcttatt gttgttgcgg
    22741 tggtcgcctt actggtaatt ggcccagaaa agctgccaaa agtagcgcgt acgctaggtg
    22801 cgcttaccgg acgtatgcaa aaatacgtgg cgcagattaa agatgaagtc aatcgggaag
    22861 tgcgatttca agagttgcag cagttgcaac aagaaatcca aacagctgct tttaatacta
    22921 aagaagccat acaatcctct ttgaatgatg taaaagcgca agtttcagtc gaggcggaag
    22981 cctttcaact cacagcgctt gacccaactc caccagcaaa acctgctaca cagccgcaga
    23041 ctaaaccagt caaacccaaa gccactcagt caggtacagc cgagttgccg ctcaaggcgt
    23101 gtaaaggtca atctgccgtt aataaaacaa ctgccgaaaa aaagcgtgtg gcaaaagcgg
    23161 ctaaagaagc atagcaacca tcgtgacccc caccgacaac ttcatttcac atctcattga
    23221 gctacgcaat cgtctgctac gcattgtggt tggctttgtt gtgatttttg ttgtattttt
    23281 tccgtttgct aacgacattt atgcgttatt agctgcaccg ctattgggca aactccccgc
    23341 tggcggacaa atgattgcaa ctgccgtcac cacgccattt tttgtgccaa tgaaggttgc
    23401 catgatggcg gcgtttatgg tgtcattgcc gcataccgtg taccagattt gggcattcgt
    23461 ggcgccaggt ctctacgcac atgaaaaaaa attcatgatt cctgtgatta ttgccagctg
    23521 tctgttgttt ctgttgggca tggcattcgc ctatttttta gtgtttcctg tggtatttgg
    23581 ctttattgta ggcaccgcgc caattggggt ggcggtcatg accgatatcg acaactactt
    23641 agattttgtg ctgacacttt tttttgcttt tggcctagcg tttcaagtgc ctatcgcggt
    23701 ggtgatgatg gtgcgttttg gctgggtgac cattgcgcaa cttaaagaag cgcgtggtta
    23761 tgtaattgtg ggggcgtttg tgattggcgc aatttttacg ccgcccgata ttatttcgca
    23821 atttatgctg gccgtaccga tgtggttgct ctatgaatta gggattttga ttgcggcact
    23881 tcagaaaaaa ccaccagaac cgtcaattcg cccacctgct gaataagtca cacgcacctc
    23941 actttagtct gcacagatgg cgaactaaaa tccactaaaa cattgaagta aaaaaagtgc
    24001 tttattcact taaaaatatg ccgaggaaaa aataatgccc tgcgagcctt tgtttataag
    24061 gctcgcaggg catcgaaaaa ggttggtatc aatattgacc taaatttaag caaaaattac
    24121 acgcaaattt agtggtattt tcttgcgtgg ctgcacattg acttcagtaa aaccacgagt
    24181 aaagaacgcg gagaatgctt ttacagctgt gtgctttact cttttaaagc aacaggtttt
    24241 ggtcttttgc caatcagaat cacaatgtcc agcatatttt catcgcgcgc aattttaacc
    24301 gttgcgtttt taaaaggcgg taatgcggca attaaattaa gcatgctaga agtatcagta
    24361 atcggtttgc catcaatgct gagtaaaatg tcgcccgccc gtaacccggc cttatcggct
    24421 gggctgttcg gcaaaacacc tgctaccagc gcaccttcag cggttttgag tttgaatgat
    24481 tctgccagtt ctggcgttaa gtcttgtgct tctatgccaa tccagccgcg cgtcacgttg
    24541 ccttgcgcaa caatttgatc catgatttgt ctggccaatg tggcaggaat cgcaaagcca
    24601 atgcccatcg agccgccgct acgtgaataa attgcgctgt tgatgccaac caagttacct
    24661 tgcacatcaa tcagtgcgcc acctgagttg ccagggttaa ttgaggcatc ggtttgaatg
    24721 aaattttcaa acgtattgat gcctaaatgg ttgcgcccca gtgcgctgac aatgccctgc
    24781 gtgacggttt gccccacgcc aaacgggttg ccaatggcca acaccacatc gcccacattc
    24841 agttggtcag atttggcaaa agtgatggca ggtaaatgtt ctgcctcaat tttgataagt
    24901 gccaagtcgg tgtcagggtc ggtgcccacg actttggcag ataatttgcg gccgtcagaa
    24961 agggcaactt caatttcatc tgcggttgct accacatgat tgttggtgag tattaagcct
    25021 tgctcggtga cgatgacgcc tgagcctaaa ctgttttctg gctgtgctgc actctcttca
    25081 tcatcaaact ggtcaccaaa aaagtggcga aatagcgggt catctaaaaa tctttgctgt
    25141 gggtttgccg ctactttttt gctggtgaag atgttcacca ccgcaggcgc tgctttttgc
    25201 acagcatcgc ggtaagaacc cgctgtagtt gcatgcggtt gcggttcaac ttgtttgacg
    25261 acaacttcat tcttttttaa tgcagaagca tcaaacagcg tcaccaataa gtccggaaaa
    25321 aacattcgca atacaaatgc tagccctaag caaaccgtca cactttgtgc gaaggtgagc
    25381 cataattttt tagagagaga atgattcatc gatataattg tcgttaactt aaggggttac
    25441 gtgttgtctg ccaacaatat gaggcagagt cccctgaaaa tcaatatggc aacttcaaaa
    25501 aggtcgtaaa aaatggtaaa aatgagcgag ttaacgcatt atacccaaca actcttgcag
    25561 gttgagcgtt ttaaagatta ttgccctaac ggcctgcagg ttgaaggtaa ggcggaagtg
    25621 cgtaaaatta tctctggtgt gacggcaagc atggctttgc tggaagctgc gcatcaggct
    25681 catgctgatt tagtccttgt gcatcatggt tatttttggc gcggtgagga tgctcgcgtg
    25741 gtgggcatta agcgcaaacg gctggctttt ttgctgcagc acgacatcaa tctgttggct
    25801 taccatttgc cgttggacgc gcatcctgaa ctaggcaata acgtgcaact aggccaggtt
    25861 ttagggtttc cattagcggg ttttgcaggc gaaactaata tgatttctca tgctacatta
    25921 acgcacacaa cgactctgcg ggcgttatcg gcgcatattg aaagcacact gcagcgcgca
    25981 ccgttggtga ttggcgaccc ggaaaaattg gtgcaaaaag tggcttggtg tacaggcgcg
    26041 gcacaaggtt atattgaaga cgccatcgcg ctaggtgtgg atgcgtttat cagtggtgag
    26101 atttctgaac aaaccactca tcaagcgcta gaatcaggcg tgtcttatat atcagcgggg
    26161 catcatgtaa cagaacgtta tggcgttcaa gcgcttggtc aacacttggc tgataagttt
    26221 ggcatcacgc atgaatttat tgatattggc aaccccgttt aattgtttta tatcaaacac
    26281 ttattaaata taactttaat tatgttttag gaaaatgtat aatgcgcaaa gttttaattg
    26341 agtatgtaat ttgcagtcaa cctttttagt tttaaaaatc tcttaaacac cattaagtta
    26401 gcctcaaaag ggcggaattg ggaatctaat gtcagccaat caaattgaca aaaacctaaa
    26461 gcaggatgta aatgagcgta gcgccgtgga tcaagaaaaa agaaaattct tgattgcaac
    26521 ttctgccgtt ggtgcggctg gtgctgcagt ggtagcagtg cctttcgtcg ccagcatgac
    26581 ccccagtgag cgagccaaag ccgctggcgc gccagttgag gtggatgtga gtaaaattgc
    26641 cccaggtgaa atgatggttt cagaataccg tgggcagcca ctttgggtaa ttcatcgtac
    26701 tgatgagcaa attgccaagc tgagtaatca taatgcgcaa ctcacagatc cggatttgga
    26761 agtgccgcag cagccggaat actgcaaaaa taaagaacgt gcaattaacc ctaaatatgc
    26821 ggtgatgctg ggtacctgta cacacttagg ttgctcacct gcgcaagact ttttgacaac
    26881 cgcagattgg gatggcggtt tcatttgtgc gtgccacggt tcaaaatttg acttggctgg
    26941 ccgcgttttc aaggggtcgc ctgcacctac caatttagtg ataccaaaac accaatacct
    27001 cactgaaaca accttgctgg tgggtgaaga taaggagtct gcataatgag tttattcact
    27061 aaaaaagcgg acgagttgtt aggttgggtg gatgcgcgct ttccgttgac cagcaatatt
    27121 aaagcgcacc taacagaata ttacgcgcca aaaaacttta atttttggta cttttttggc
    27181 tcattagcgc tgttggtgct ggtgctgcaa attgtcacag gcatcttttt aacgatgcac
    27241 tataagccag aagcgctcat ggcctttgct tcagttgaat acattatgcg cgatgtgccg
    27301 tggggttggc tcattcgtta tatgcactca acgggcgcgt cactattctt tgtggtgatt
    27361 tacctgcaca tgttccgtgg tttgttgtac ggctcatacc gaaatccgcg tgaattgatt
    27421 tggttatttg gggtgggtat tttcttggcg ttgatgggag aggcgttttt tggctaccta
    27481 ttaccttggg gtcaaatgtc ttactggggc gcgcaagtga ttaccagttt gtttggtacc
    27541 gtgccgttca ttggtccaga catcgcagag tggttgcgcg gcgattattt aacctcagat
    27601 gcaacactca atcgcttttt tgcgtttcac gtaattgcac tgcctttagt gattttaggc
    27661 ttggtggcag cgcatattct tgctttgcat gaagtcggtt caaacaatcc ggatggcgtg
    27721 gaaattaaaa agttgaaaga taaaaaaacg ggtattccgc tcgatggtat tccttttcac
    27781 ccttactaca ccgtgaaaga tatggtgggt gtggtggtgt tcttgattgt gtttgcggca
    27841 attattttct ttgcaccaga aatgggtgga tatttcttag aagcgaataa ttttgtaccg
    27901 gctgacccgt tggtgacacc ggcgcatatt gcaccaacat ggtatttcac accttactat
    27961 tcaattttac gcgccgtgcc gccaattggt atttcgcaat ttccaggagt tgctgcaatg
    28021 ggcttggctg tgctggcatt tgccttcttg ccgtggttag atagaagccc agttaaatcc
    28081 attcgctata aaggtaatct ttacaaaggc tggttggcag catttgttgt aagcttttta
    28141 gtgttgggtt atctcggaac cgtgcctgca aacgtgtggg gtcaatttgg tgcatggtta
    28201 ggtaaagcgg atattgcaac cgtggtggcg cgtattttca cagtggttta tttcgcgttc
    28261 tttatcttaa tgccgtggta caccaaaaaa gataaaacaa aaccagtgcc agaaagggta
    28321 acggggtaat catgatgaaa atacaaatga ttaaaaaagc catttatgta ttggcaattg
    28381 tgccgctact agcattcgca aatgtagaag tacatttaga taaagcgcct atcgacccat
    28441 cagatcaagc atcattgcaa cgcggcgcac gcacgtttgt taataactgc ttaaattgcc
    28501 acagtgcaaa ctacatgcgc tataaccgtt tgcaagatat tggcttaacg gaaaagcaaa
    28561 ttaaagaaaa cttacttttc accgctgaaa aagttggtga cccaatgaca tctgcaatga
    28621 acaaaaaaga tgccaaaaaa tggtttgggg tagcgccacc tgatttgtct gtagaagtac
    28681 gcgctcgcgg cgctgattgg gtttatacct atatgcgcag cttttaccgt gatgaaacgc
    28741 gcccgtcagg gtggaacaac gtggtatttg ataaagtggc g
//
LOCUS       DAOQSI010000009        26376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_1142_length_26376_cov_5.925307, whole genome shotgun sequence.
ACCESSION   DAOQSI010000009 DAOQSI010000000
VERSION     DAOQSI010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 26376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26376
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1142_length_26376_cov_5.925307"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..373
                     /locus_tag="PKL42_01440"
     CDS             62..373
                     /locus_tag="PKL42_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HNU65761.1"
                     /translation="MNISALLLAISLSFGFSQLSLADHHEDGGKHCSRHHKMQDADTN
                     KDGAISRDEFMAGHQAMADKMFSKMDANNDGKIDEAERTAGKEKMGKHCDRKDHDKSS
                     K"
     gene            502..1194
                     /locus_tag="PKL42_01445"
     CDS             502..1194
                     /locus_tag="PKL42_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNU65762.1"
                     /translation="MHAYTVLITGANRGIGLELSRQYAADGWQVLACCRHPQSAIALQ
                     ALTTQHDNVHVLPLDVSDFRQIDALALTLKAQNIDVLINNAGVYPESTFGDTDEQLWA
                     NAFKVNSMAPMKMAEAFVQHITRSQLKKIATLSSKMGSIDDNTSGESYLYRSSKTAVN
                     MVMKSLAIDLKPYGISVVTLHPGWVRTDMGGPNGLIDASASATGLREVIAHLNLSNSG
                     RFIAYDGKPIAW"
     gene            complement(1160..2353)
                     /locus_tag="PKL42_01450"
     CDS             complement(1160..2353)
                     /locus_tag="PKL42_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNU65763.1"
                     /translation="MANADNGLILKPMSRIISIVQTIEQVSASDWNALVGDMPLLSYA
                     FLSALEHSKSIGAGTGWQSYYLLVKEDALLVGAMPLFIKSHSYGEYVFDWAWADAYAQ
                     NGLSYYPKLLSAIPFSPVTSARLLSATVEVQTLMLEALDTVMRQLQLSSAHVLFPDEH
                     SAAVLNKAGWLQRHGVQFRWQNHQYQNFEEFLNTLSHDKRKKIHQERKKVQASGVVCH
                     AVLGKNISAELWDFFYTCYSNTYAEHHSTPYLSRAFFSEVGQTMPHNIVLMVASRNNQ
                     PIACALNFYNQTTLYGRYWGATQFVSGLHFELCYYQSQQFCITENITYFEGGAQGEHK
                     LARGFEPRPTCSYHRIAHAEFAEAIAQFLAREQENITVYTNELEERAPYKAATTKQSA
                     FHHKQ"
     gene            complement(2424..3059)
                     /locus_tag="PKL42_01455"
     CDS             complement(2424..3059)
                     /locus_tag="PKL42_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HNU65764.1"
                     /translation="MLNNFSISKCACVAACIYLGLSNYALAAGRGYFFVVGDSQRLAA
                     NEDASAEEGLQQTLNLNFSPENREKLRRALDDYARSVDENHEQIEARRKAMHESIEAR
                     FLDADTDGDGMVTRQEATEKLPQIARHFNQVDTNEDGAITIDELEEAQARILERRKAA
                     EMAIEEKKLQAAVEAVVNKQAETAPAKPIEAIPSKHKDARQTSVNLKKRSY"
     gene            3430..4152
                     /gene="ompR"
                     /locus_tag="PKL42_01460"
     CDS             3430..4152
                     /gene="ompR"
                     /locus_tag="PKL42_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HNU65765.1"
                     /translation="MSDQHKKILVVDDDVRLRELLQRYLTEQEYTVKVASDAKEMDAI
                     LAVEAIDLLVLDLMLPGEDGLSICRRIRASGTLLPIIMLTARGDEVDRIIGLEMGADD
                     YLPKPFNPRELLARINAVLRRHERLQPSAPALNSDSVTIGEFVFNPATRSLTKDGAAV
                     TITSGEFALLKVFVDHPRQPLSRDKLMQLARGRELDVFDRSIDVQVSRLRKLIEPDAA
                     HPRYLQTMWGFGYVFIPDGEVD"
     gene            4262..5533
                     /locus_tag="PKL42_01465"
     CDS             4262..5533
                     /locus_tag="PKL42_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNU65766.1"
                     /translation="MLLIAVLLAVGQFASLQIFEYFERKPRAEATALQAVTVVNYTRA
                     SLIASQENLRQALLSEITGKEGVRVYYADFMEEIEPLPSDPFINMVAEKIRERLGTET
                     IITTRHYGIEGLWVSFNIGLDDFWVVIPRVHVDRPFPWQWLGWGALVLALSLAGGYLI
                     AMRINRPLRLLMNAADRLRNGESPEKLPVGSFAELQEVNDTFNKMADSLAELDAERTL
                     ILAGVSHDIRTPLARLRLAVEMLPDESCASFKAGMIEDITDMNNIIHQFLDFVKGVEG
                     EPTQMVDINNLLQGIHDRQLRAGRDISVELGPTYAIPIRPLAMQRLLDNLVGNAYAYG
                     RGQVRIASRITADYVVISVFDNGPGIPETHVEKLLRPFERLDTARSNAGGSGLGLAIA
                     DRIAKLHQGKLELINRTEGGLEARLSIPIYS"
     gene            complement(5536..8943)
                     /locus_tag="PKL42_01470"
     CDS             complement(5536..8943)
                     /locus_tag="PKL42_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNU65767.1"
                     /translation="MRKLRERFILATVLSYVVLALLWVFLSDHLLSTFTTIDSIHWYS
                     TEKGVFFVLVSALALLFVLRTVPSVANDHQGHLQDVIFSGAFISRRPTWLTYSFAVLI
                     SLVMLLIRHHMGLNTDHRPLMILFMFPIILGALLGGFWPGVISTATVAIGAKLLFVPT
                     RWSFQFTDGYDLLQWSFLIVSGLTMSVLSEILMRTRAKAENQAKLLNVAVSGTRDAIY
                     VKDRAGRYLLINQAGADIVGKPMDEIIGQDDSFIFPEPTASHLKRLDAEIMAKGKTQT
                     HEEQVSLLNAGTITAYVTKGPMFDDAGNVIGLFGISHDITEIKQVQTALQDRDFKLSA
                     IVNYSPAFLSLKDTAGHYVLANPKLLRIHGLTELEILGKTDFDLYPEEIANNLSINDE
                     IVLCTMQRHAIEEFWPVAGELRYFASTIFPVLNTSGAIQYICRISLDITDNKQKNERL
                     QKSEARLKEANHFARIGDWSWDTVTNTHVWSEELYEIYGRDLALPPADIDEVPQYFTV
                     KSWQQLTVVMNQGLLDGLPYECDAEVIRADGTRRWVTARGKGIHDENGQIVGLHGTVQ
                     DITERKQAEINLQISAVAFESQDSVMITDASLRILRVNKAFTEIFGYTTEEVIGETPR
                     LLQSGTHDTDFYSDMWQKIRREGSWQGEVLNRHKNGQLLYNLVSISAVKGNDGVVTHY
                     VGSHVDITARKAAADEILHLAFHDLLTQLPNRQLFIDRLQHAIASSARTRQIGALLLI
                     DLDNFKALNDTLGHDVGDLLLQEVAKRLSSSVREGDTVARLGGDEFVVLLENLSEQLN
                     EAASLAEVVAHKIQTMLNEQYQIAVHQYHATSSIGVTLFQGNMLEIAELLKQADISMY
                     QAKKSGRNRIRFFDPKMQEAFNVRASLEIELRSALEFQQFQLHYQVQVDEFGKAIGAE
                     ALLRWNHPSRGMVPPLQFIPLAEETGLILPIGQWVIESACAQLEQWQKNPMTQHLTLS
                     VNVSARQFSQAEFVRQLQLTLQRYTFRPKQLKLELTESLLLENVESIIISMIALESIG
                     VLFSLDDFGTGYSSLQYLKILPLNELKIDRSFVRDIADDSNDLSIVETIVAMASSLGF
                     DVIAEGVETEEQKRLLLNKGCKHLQGYLFGKPMPLDQFEATLQTKAH"
     gene            complement(9044..10441)
                     /gene="cysG"
                     /locus_tag="PKL42_01475"
     CDS             complement(9044..10441)
                     /gene="cysG"
                     /locus_tag="PKL42_01475"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041353255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HNU65768.1"
                     /translation="MQALPIFMNITQRLCVVVGGGEVALRKVSLLLRANATVKLFAPE
                     ICLQLQALVDAQKITYIPAHFEPQQINAACLVIAATDDEAVNQAVSVAAKAQNIPVNV
                     VDAPALCTFTMASIVERAPIVIAISSEGTAPVLARYIRTKIETMLPAGYGRIAAIAGE
                     FRDKVKAKFATSQERRIFWEGILQGPIVERILAGQESAARLALEKAIADQPDTQQMGE
                     VYLVGGGPGDPDLLTFRALRLMQQCDVCVYDKLVSPEVMDLVRRDAELIYVGKSRDQH
                     TLPQEEINDLLAKLALEGKRVLRLKGGDPFIFGRGGEEIETLMQQKVPFQVVPGISAA
                     NGVSSYAGIPLTHRDYAQACLFITGHLKDGTLDLDWVAMSRPRQTVVIYMGLVGLAQI
                     CEQLIAHGVSPEMPAAVVQQGTTQRQRVVTASLKDLAQQVAQANIKAPCLTIVGEVVR
                     LRERLNWFEPEANVQ"
     gene            complement(10481..11104)
                     /locus_tag="PKL42_01480"
     CDS             complement(10481..11104)
                     /locus_tag="PKL42_01480"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNU65769.1"
                     /translation="MFRLVTMLWLMLFFSFSALADYDEGVSAYEKANYGLALKAFEQS
                     AKAGHTDAQTYLGVMYELGQGVKMDYEKALMWYRKAAAEKNPTAEYNLGQMYFYGRGV
                     KEDQKQAVVWYRKSADQGYASAQSMLGYTYDVGAGVAQDYQQALNWYLLAADQHDVDA
                     QYNLGNMYNEGRGVERNRKEAFYWFRLSALQGDVEAKQALKELLRAK"
     gene            complement(11331..11768)
                     /locus_tag="PKL42_01485"
     CDS             complement(11331..11768)
                     /locus_tag="PKL42_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65770.1"
                     /translation="MTENSNQHNAIELTQAELHHPAWDEPIICRVEVDLPAWLTQLTG
                     GRQWEVYSEAEEENCISFEMRESQHAMTKTGGTKPTGKLQATDKAEAATPKLAEVTLY
                     HNGYAVVDVDGKSLFDGSLTSGGSSFAHLTYFHAESGEKITLN"
     gene            11955..12824
                     /locus_tag="PKL42_01490"
     CDS             11955..12824
                     /locus_tag="PKL42_01490"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HNU65771.1"
                     /translation="MLSGLSEISTLLAQNSTLFITISVIFGLLVGSFLNVVIYRMPKI
                     MEREWHNNCLELQGLEIPEQPKFSISKPRSACPHCGHKITALENIPVISYLFLRGKCS
                     SCQAKISPRYPLIEALTGLLIGLVSWKFGYSSLTLFAWLFTFALIALTFIDFDTQLLP
                     DDITLPLLWLGLFVNLNSGFTDLKSAVIGAIAGYLILWSIYWIFKLVTGKEGMGYGDF
                     KLLAAIGAWFGWQLLPAVILLSSIVGVIIGVGLIIYAKRGREVPMPFGPFLALGGIAA
                     LFYGQQLAGYYLH"
     gene            12824..13432
                     /gene="coaE"
                     /locus_tag="PKL42_01495"
     CDS             12824..13432
                     /gene="coaE"
                     /locus_tag="PKL42_01495"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831464.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNU65772.1"
                     /translation="MSIIALTGGLGSGKSEAAKQFAKLGVPVVDTDVIAHALTANGAP
                     ALKQISELFGPQFLTNEGALDRAKLRAHVFAHPQERSKLEALLHPLIRAAALKQLAEN
                     EHQQHPDYQILVIPLLFENNQYQTSIHKILLIDCDEQLQIARAMARSFLTQAEAQAIL
                     DAQVSRTTRLKLADEVIENNGTLAELTESVEKMHQKLIKICH"
     gene            13514..14272
                     /gene="zapD"
                     /locus_tag="PKL42_01500"
     CDS             13514..14272
                     /gene="zapD"
                     /locus_tag="PKL42_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HNU65773.1"
                     /translation="MPSYDYPFNERIRTLLRIEDLFAKVMHNLETDHEFHHHAALLTL
                     LQISDIVERADLRSELLQELARQKLVMDSLKGNPAIASAKLESILAEIDTAVDALRAE
                     NTKLGQTLRNNEWLMAIKQRTGIPGGVCEFDVPSYHYWLNLGPTKRRKDIVAWLNPLL
                     PMQQAITIILHILRGSGAKTKLLAKAGVYQEMLSGAKPAQMLTIEIADNTPCFPEVSA
                     NKYAMNIRFIHLDNAQKTQKYEGDVKFTLTLCNL"
     gene            14347..14556
                     /locus_tag="PKL42_01505"
     CDS             14347..14556
                     /locus_tag="PKL42_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HNU65774.1"
                     /translation="MSTIKKRLVTCPNCKKLSEYSLENAFRPFCSERCKMIDLGVWAT
                     EGYAIPVAINAADLSDDETNALLGH"
     gene            14568..15038
                     /locus_tag="PKL42_01510"
     CDS             14568..15038
                     /locus_tag="PKL42_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HNU65775.1"
                     /translation="MQLLTLFSRLCTTTLLPTVLMTVFFTAHAETAKPVQIQQAWARA
                     TAPGQNVGAAYMTLTHHENITLLAADSDAAGTVEIHSMEMKNGVMKMRMLEDLPLKAG
                     KPVKLGPGGLHLMLFDLQKPLKVGEKIDLTLCFKDQRGEVTHQTVTLPIKEAAQ"
     gene            complement(15061..16023)
                     /locus_tag="PKL42_01515"
     CDS             complement(15061..16023)
                     /locus_tag="PKL42_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HNU65776.1"
                     /translation="MTTNLDSVSPSAAVTHAAVGVIQRQDGAVLLGERPAGKPWAGYW
                     EFPGGKIEENETPARALMRELHEELGITVTEMAPWLTRVYAYPEKTVKLHFFRVLAWL
                     GEPHGREGQQLSWQQPSQVRVQPMLPANQPIMQALNLPDVYAITNLAEMGEQAFFNQL
                     QLALANGLKLIQIREKQLQPAALQTFSERVIHLAHARSAKVLLNANAELVRELGADGV
                     HLPAHQLLELRQRPDDMLCAASCHNLAELEKAATLGLDFVVLSPVNATLSHSDAIPLG
                     WQAWGQMIADYPLPVYALGGVRIDDKKTAVQHGAHGIAMQRGAW"
     gene            complement(16031..16906)
                     /locus_tag="PKL42_01520"
     CDS             complement(16031..16906)
                     /locus_tag="PKL42_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNU65777.1"
                     /translation="MDKLTHLIERAEGLINQLENLLPAKISPVDWQAFAWRWVKQNNQ
                     GRLQAVAHPHHIALADIYFVDAQKTQIVRNTQQFLQGFTANNVLLTGARGTGKSSLIK
                     ALLTAYAAEGLRLIEVEKHDLVHLPEIVEIIRNRPEKFIIFCDDLTFDANDESYKALK
                     VVLDGSVASASDNMLVYATSNRKNLMPEFMADNLATKHWDGEIRPSDTIEEKTALAER
                     FGLWLSFYAFDQEEYLAICATWLKHFGFEFDAAAHEAALKFTHTRGARSGRVAYQFAR
                     DYAGIKQLALSTLRQ"
     gene            complement(16906..18132)
                     /gene="argJ"
                     /locus_tag="PKL42_01525"
     CDS             complement(16906..18132)
                     /gene="argJ"
                     /locus_tag="PKL42_01525"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HNU65778.1"
                     /translation="MPVNLTAPQASALFPVKGVKLGCAEAHVRKPNRKDVLVVTLAES
                     SHVAGVFTLNQFCAAPVLLCKEFLKQKAGIRALLVNTGCANAGTGEDGLNRAQQSCQA
                     LGDLLNLQANQILPFSTGVILEPLPVDKVIAGLPAAIANLKEDNWLNAAEAIMTTDIV
                     AKGASRQIQLGGKTVTLTGISKGSGMIHPNMATMLGYIATDAAVSQSALESIIQYAVN
                     KSFNCITVDGDTSTNDSLILMATHLAGNAEITETSPEFAVLRDAFTELAIELAQAIVR
                     DGEGATKFMTVQVEGGRDAAECRKVAYAIAHSPLIKTAFFASDPNLGRILAAIGYAGV
                     EGLDVNALKLYLGDVLVAEHGGRAPAYQEAQGAAIMQKAEILVRVDLARGSAQSTVWT
                     CDFSYDYVKINADYRS"
     gene            complement(18370..21618)
                     /locus_tag="PKL42_01530"
     CDS             complement(18370..21618)
                     /locus_tag="PKL42_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNU65779.1"
                     /translation="MDLSVVYAKTPKGVRLRKTLFGGLPAHIMKIMALIDGKTSAQNI
                     LNQLEGFSEQKFIAAISQIENEGYIKIVEVVAQEDDWELDTLFSPMVVEEVYNVEEID
                     GTEELETYLELEQIAKKKEAERREDELKAREVEAQIRAKEIAKAEVKIRQKDAEEEAH
                     RAQQAEIERQKAEAETRKQAEMKAKAQAKVDVQEKARLEAEHLAREAELARIRAEAEA
                     AQAKAKLENERQERLAAEARAQAEKVVLETAEKERAIAEAKENAKRAAEEDARLAAAR
                     IAEKKAQAREQAEHAARQKAEHAAQALVERIREKERQRREETRKMKEAEEVRMQVALA
                     LQAKAQAEEADRLAGEKKAQEQQATAEALAKAEEAARLEAEHQAKLAEEARLQAELAA
                     KQEAERLQRIAEAEEVARVAAEEQAHLEAARIAQAAEEARLQAELEAQAQAEEAARLE
                     AEHQAKLAEEAQAQAEAAARLEAERQEKLAQEAQAQAELAAKQEEERLQAIAAAEEAA
                     RLAAEAQARLDAERMAREAEAAQKKAEADARALAEKEAKIEAERQARLIAEANAKEKA
                     AAKEQAKLEKARIAKEKTERETQKKAEAKAQKEAERLAKKEKEVKQAAVKAEEQAAEK
                     AAREEAKSLASEMMGHKPLRAKNSLLNVAKAKYRKFDILVLSRFKGANDNAKQPKKWL
                     SGLLRFSLVYMPIALILLLGLMHFVPLNYLVAPIENFAAASIGAPVKVGEVRASLWPQ
                     PHFMLHHVMMGENPAIQLDSIKAAPQITSLLSEVKTLHSLLIEGVTLNQKDVAKPLQW
                     LQAARQSPQLQVEDVRFSKVKLNIRDLTLVPFNGHVMQHHAGELGGIALKSEDGALAI
                     EVQPQANQYRVKIAASKWTLPVRPNIVFEALHASGTYQQERLQLDQLQGEIYGGTFSA
                     KAMVDWSNQWQTSGNFNLIKANAAQLLKAFSSTALVDGKLNLAGKFSAQSNIATQLLD
                     DAEVTGNFNIPNGKINGVDLARAILNPEDKALEGYATNFDQLTGGVWAKSGRVEYRNL
                     VLKSPKLQAQGQVQIAPSEAISGKISLNAVAQGRNFQSTFDLTGKVSNVKRY"
     gene            complement(22013..22687)
                     /locus_tag="PKL42_01535"
     CDS             complement(22013..22687)
                     /locus_tag="PKL42_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HNU65780.1"
                     /translation="MFFHLLLIFEYVFSSRFVKRLNLSLFGFIFVALSTHALAENSKI
                     LVYGDSLSAAYGIAQPQGWVTLMQQKLNAEGYHYEVANASISGETTSGGLTRFEKTIT
                     TTRPMIVILALGANDGLRGLPIQEMHKNLSAMIAYSQKNKAKVLLLGMRIPPNYGPKY
                     TEAFFQTYQQLGQQHKISLVPFMLDQVAAKPDLVQADGLHPNALGQPVILKNIWPALK
                     LMLKKK"
     gene            22690..23376
                     /locus_tag="PKL42_01540"
     CDS             22690..23376
                     /locus_tag="PKL42_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNU65781.1"
                     /translation="MQSSPIVQAQALTYEFVSHDNHIRVLYDLNLSIAAGEQVAIIGS
                     SGSGKSTLLSLLAGLDIPTSGSVQIDGKSFSQLDEDGRAAIRAKRMGFVFQSFQLLPS
                     LTALENVMLPLQLKNHPDAASLSLAALRQVGLSERVQHYPKQLSGGEQQRVAIARAFA
                     TQPAILFADEPTGNLDHNTGQRIIELLFEMNANAGTTLVLVTHDIQLANRCHTQLQLA
                     HGTLTRLNQP"
     gene            23373..25880
                     /locus_tag="PKL42_01545"
     CDS             23373..25880
                     /locus_tag="PKL42_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNU65782.1"
                     /translation="MSVTLKLAWSQTMSLWKSGALNVLVFALVLAVTAITATGFFTQR
                     IETALNQQGGLLLGGDLAVLADHEIPETFINNAKTQGITTVKTYEFPSMVVFGEANQL
                     AEIKAVEAGFPLRGDLTIGQHATDTGKIVNQGPNTGEVWIEPRLVNLLNIQVGDRLEV
                     GERQLRVSAILVREPSRGGDMFGFAPRLMMHAADLPSTKLIQYGSRVKYQLLLAGQPQ
                     SISQYFEQMKPQLNRGEKIQDIRNARPEIKSALDKAQQFLGLSSMVSVILAMVAMLLS
                     SLPYIKQSLDTFALMRCFGASKNNVLQVLAFQTTIIALLSALFGAALGYIGQLGLATL
                     AGNLFVETLPAANATPLFLGFLASLGMMLAVVLPHAWQMRNLTAMNILRRETISQPLS
                     TQAKFLPAFFVMTLMIFWQARDIKLALSTIIALTLLCMIIAGIAYLLVSLVSRVFRLS
                     SRNQMLGQIKIGILGIKRRFGLSTVQMIGFSMGLMVLMLLALIRNDLIRNWQASLPID
                     APNRFVINIQPAQISGIKEFFTQQKIQDATIFPMVRGRLISQNNKDLSQTEWKDERAK
                     RLAEREFNLSWAANMQSDNKLLSGRWWTEQDHGKPYLSLEEDLAKTLNLKLGDQLVFN
                     VAGTPLNLTVTSIRKVEWDTMRPNFFAVTPPGVLDQYPTSYMSSFYLPLSADAPMNQL
                     IKTYPNLTVIDVAALMQQVRGIMQKMSSAIEYVFAFSLMAGIAVLYAALIATRDERVT
                     EATLMRVFGASRQQVSVAYIAEFACIGLIAALVAAIAANLLAYYLSHFVLDIPFQLNF
                     TLAVTVMLIAAICIPLAAWLVLRKFLNVPARQLLNSI"
     gene            complement(25955..26260)
                     /locus_tag="PKL42_01550"
     CDS             complement(25955..26260)
                     /locus_tag="PKL42_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65783.1"
                     /translation="MSTLLRALLALSVTSLLLVGCGEKEAAPVEAAPAVEAPAPVEAA
                     PVEAAPAPAPAAAEPGGYEPTPEERVPGITLDDSAQPVTEASAPAEAPAVEAAPEAK"
BASE COUNT         6894 a         6471 c         5692 g         7319 t
ORIGIN      
        1 taagccttac tggcaggtct acgttaagta atagcaacat ttaatttacg aaggaataca
       61 aatgaacatc tcagcactac ttttagccat ttcgctcagt tttggattta gtcagttatc
      121 gttggcggac caccatgaag acggtgggaa gcactgttcg cgtcatcaca aaatgcaaga
      181 tgcggatacc aataaagacg gtgcaattag ccgtgatgaa tttatggctg gccaccaagc
      241 catggcagac aagatgtttt ctaagatgga tgccaataac gatggcaaaa tcgacgaggc
      301 cgagcgcacc gcgggcaaag aaaaaatggg gaaacattgc gatagaaaag accatgataa
      361 gtctagtaag taatcatatg caatcagctt aaacaaccga aagcaggtaa aaactgcgca
      421 atcaagccac acttatttag tgtggctttt tgtttttatc acgcgcataa ttgcccaaca
      481 ttaatgtgaa tggagtcaag catgcatgca tacaccgtgt taattaccgg ggcgaatcgc
      541 ggtattgggt tagaacttag ccggcaatat gccgccgatg gctggcaagt attggcgtgt
      601 tgccggcacc cgcaatcggc cattgccttg caggcgctca ctacacagca tgacaacgtg
      661 catgtgttgc cattagatgt gagtgatttt aggcaaattg atgcactggc actcacactc
      721 aaggcgcaga atattgatgt gctgattaac aatgcgggcg tttatcctga aagcactttt
      781 ggcgacaccg atgagcagct ttgggcgaat gctttcaagg taaacagcat ggcgcccatg
      841 aaaatggccg aggcttttgt gcagcatatc accagaagcc agcttaaaaa aattgccacc
      901 ctcagcagca aaatgggcag cattgatgac aacaccagcg gtgagagtta tttgtatcgc
      961 agcagtaaaa ctgcggtgaa tatggtcatg aaaagtttgg cgattgacct caagccttac
     1021 ggcattagcg tggtgacgtt gcacccgggc tgggtacgca ccgacatggg cggccccaat
     1081 ggtttaattg atgccagcgc cagcgcaacc ggcttgcgtg aagtgattgc ccatttaaac
     1141 ttaagcaact caggccgatt tattgcttat gatggaaagc cgattgcttg gtagttgcgg
     1201 ctttgtaagg cgcgcgctct tctagctcgt tggtgtagac agtgatattt tcttgctcac
     1261 gcgctaaaaa ttgcgcaatg gcctcagcga attcagcatg agcaattcgg tggtatgaac
     1321 acgttgggcg cggctcaaat ccacgcgcca atttgtgttc gccctgcgca cctccttcaa
     1381 aatatgtgat gttttcagtg atgcaaaatt gctgtgattg gtaataacac aactcaaaat
     1441 gcaagccaga aacaaactgc gttgcacccc aatagcggcc gtacagcgta gtttggttat
     1501 aaaaattcag ggcacaggca atgggttggt tattgcgcga tgcgaccatt agcacaatat
     1561 tgtgtggcat cgtttggcca acttcactaa aaaacgcacg tgataaataa ggcgtagagt
     1621 gatgttctgc ataggtattg ctgtagcaag tgtaaaaaaa atcccaaagt tctgctgaaa
     1681 tattcttacc taaaactgcg tgacatacca cgccagaagc ctgaactttt ttacgctcct
     1741 gatgaatttt tttacgttta tcgtggctta acgtgttcaa aaattcttca aaattttggt
     1801 actgatgatt ttgccatcta aactgcaccc cgtggcgttg caaccaacca gccttattca
     1861 gcacggcggc agaatgttca tcaggaaaca gcacatgggc tgacgacaat tgcaactgcc
     1921 gcatgactgt atccagcgct tcaagcatca acgtttgcac ttcaaccgtt gcacttaata
     1981 atcttgcact ggttacgggc gaaaaaggaa tcgcagataa taactttggg taatacgaca
     2041 ggccgttttg cgcataagca tctgcccagg cccaatcaaa cacatattca ccatacgaat
     2101 ggcttttaat aaaaagcggc atcgccccga ccaacagcgc atcttcttta accagcaaat
     2161 aataagattg ccagcccgtg ccagccccaa tcgattttga atgctcaagc gcactcaaaa
     2221 atgcataact gagtaagggc atatcaccca ccaaggcatt ccagtcactg gcactgacct
     2281 gctcaatagt ttggacgata gaaataatgc gggacatagg ctttaatatt aagccattat
     2341 cggcgttagc catgcggaat tagaaaaaat ttatatgtcg atgatgttgc tgtaaatact
     2401 tgatacgggt ttaccgtttg aaattaatac gagcgctttt ttaaattgac agaggtctgc
     2461 ctagcgtctt tatgcttact tggaatggcc tcaatcggtt tggctggcgc agtttcagct
     2521 tgcttattca cgaccgcctc taccgccgcc tgtagctttt tttcttctat ggccatttca
     2581 gcggcttttc tgcgctctaa aatacgcgct tgcgcttctt ctaattcatc aatcgtaatc
     2641 gcaccatctt cattggtatc tacctgatta aaatgccggg caatttgtgg gagtttttcc
     2701 gtggcttctt gtctagtgac catgccatca ccatcggtat ccgcatctaa aaaccgagct
     2761 tcaatacttt catgcatggc ttttcgccgc gcttcaatct gctcatgatt ttcatcaaca
     2821 gagcgggcat aatcatccaa cgccctgcgt agtttttctc tattttctgg gctaaagttt
     2881 aagttgaggg tttgttgcag gccttcttcg gctgaggcat cttcatttgc tgcgagtctc
     2941 tgactatcgc ccaccacaaa aaaataaccc cgacccgcag caagtgcgta gtttgacaat
     3001 cctaaataaa tacaggcggc tacgcatgca cacttactga ttgaaaaatt atttagcatg
     3061 accatgacta agttttacta ggagcaattc aacaacctta aaaaaagtaa attcaccgaa
     3121 aatttagaac aatagtataa ccatatacta tggatggcta ttgttaatca tatgtttcag
     3181 tcttggaaaa attgtaataa tttgtaacag cattaagatt aaaccttgcc acttgcaact
     3241 gtacaaaaaa attaaaacgc tgcgtggcgt agacttgtta gaaataaatt agtttcgtcg
     3301 ttttaatacc gatacgtatt gatatccacg aaaattaact tacattttgt caccaatcac
     3361 aatatacttt aagacttaaa ttcgtggcga tattttggac agttatcaac aattaacgag
     3421 aacaacatca tgtcagatca acataaaaaa attctggtcg tcgatgacga cgtccgtcta
     3481 cgtgagctgc tgcagcgcta ccttactgag caggaatata ccgttaaagt ggcatcagat
     3541 gccaaagaga tggacgccat actggcagtg gaagccattg atttattagt gttagattta
     3601 atgttgccgg gcgaagacgg cttgtcaatt tgccgcagaa tacgcgctag cggcacttta
     3661 ttacccatca tcatgctcac agcgcgtggc gatgaagtgg atagaattat cggccttgag
     3721 atgggcgcag acgattatct gccaaaacca tttaacccgc gtgagttgct agcgcgtatc
     3781 aatgccgtat tgcgtagaca tgagcgcctg caaccaagcg cgcctgcact caatagcgat
     3841 agcgtgacca ttggcgaatt tgtattcaac cccgcaaccc gctcattaac aaaagatggc
     3901 gccgcggtca ccattaccag cggggaattt gcgctgttaa aagtgtttgt tgatcatcca
     3961 cgtcaaccgc tttcacgcga taaactcatg caattagcgc gtggccgcga attggacgtt
     4021 tttgaccgca gcattgatgt gcaagtatcc cgtttacgta aactgattga acctgatgcc
     4081 gcgcacccca gatatttaca aaccatgtgg ggctttggct atgtgtttat tcctgatgga
     4141 gaagtcgatt aaacttacgt gttataccgc atgttgctaa aaaccaatcc gattcaattg
     4201 acgttatcat caatttaaat tgacagtcca actcctccct aaaaccctgc ttgccagagt
     4261 gatgttacta atcgctgtat tactggcagt cgggcaattt gcatcgttgc agatttttga
     4321 gtattttgag cgcaagccgc gtgctgaagc caccgcactg caggcagtca ctgtggtcaa
     4381 ttacacgcgc gcttcattga ttgcttcgca agaaaatctg cgccaagccc tactctctga
     4441 aattacaggt aaagaaggtg tgcgtgtcta ttacgcagac tttatggaag aaatcgagcc
     4501 gctgccaagt gacccgttta ttaacatggt tgctgaaaaa atacgcgaaa gattaggcac
     4561 agaaaccatc atcaccacgc ggcactacgg cattgaaggg ctttgggtca gctttaatat
     4621 tggtttggat gatttttggg tagtcattcc acgcgtgcat gttgaccggc cttttccttg
     4681 gcaatggcta ggctggggcg cattagtact ggccctttcg ctcgcaggcg gctacctaat
     4741 tgcgatgcgt atcaatcggc cattacgctt actcatgaac gctgccgata gacttcgtaa
     4801 cggcgaatct ccagaaaaac tgcccgttgg cagttttgca gaacttcagg aagtgaatga
     4861 tacttttaac aaaatggcag attcattagc cgagttagat gctgaacgta cgcttatctt
     4921 agccggggtt tctcacgata ttcgcacacc cttggcaaga ctacgtctgg cagtagaaat
     4981 gcttcctgat gaaagttgcg ccagtttcaa agcgggcatg attgaagata ttaccgatat
     5041 gaataacatc attcatcaat ttttagactt tgttaaagga gtcgagggtg aaccgacgca
     5101 aatggtcgac atcaacaact tattacaagg catccatgat cgccaacttc gcgccgggcg
     5161 cgacatttcg gttgagcttg ggcccactta cgcaatcccc attcgcccac ttgccatgca
     5221 acgcctgctg gataatttag tggggaatgc ctacgcctat ggacgtggcc aagtacgtat
     5281 tgccagcaga atcactgcgg actatgtcgt catcagtgtg tttgataatg gccctggcat
     5341 tcctgaaacg catgtagaaa aactactgcg gcctttcgaa cggctagaca cggcccgtag
     5401 caatgctggt ggcagcgggt taggcttggc cattgctgac cgtattgcca aattacatca
     5461 gggaaaactt gaactgatta accgaactga aggtggctta gaagcgcgcc tgagtattcc
     5521 tatttatagt taagtctagt gcgcttttgt ttgtagcgtt gcttcaaact gatccagagg
     5581 catcggtttt ccgaacaagt agccttgcaa atgcttgcag cctttattaa gtaacaggcg
     5641 cttttgttcc tcggtttcca caccttcggc aatcacatca aaaccaaggc tacttgccat
     5701 tgcgacaatg gtttccacaa tggagaggtc attactatca tccgcaatgt cacgcacaaa
     5761 tgaacgatct attttcaact cattaagtgg caaaatcttc aaatactgca aagatgaata
     5821 gccagtacca aaatcatcca aggagaaaag cactccaata gactcaaggg ctatcatcga
     5881 aatgataata ctctcgacat tttccagcag aagactttct gtcaattcca acttaagctg
     5941 cttaggtcta aatgtatagc gttgcaatgt aagctgcaac tgccttacaa actctgcctg
     6001 actaaattgc ctcgcgctga cgttgaccga taaagtcaaa tgctgcgtca ttggattttt
     6061 ttgccactgc tccaattgcg cacaagcact ttcaatcacc cattgcccaa ttggcagaat
     6121 caaccccgtt tcttctgcaa ggggaataaa ctgcaatggt ggcaccatgc cgcgagaagg
     6181 atgattccaa cgaagcaacg cctccgcgcc aatcgccttg ccaaattcat ccacctgaac
     6241 ttggtaatgc agttgaaact gctgaaactc cagtgcacta cgcagctcta tttcaagtga
     6301 agctctaacg ttaaaagctt cctgcatttt agggtcaaaa aaccgtattc tgtttcgtcc
     6361 tgatttttta gcttgataca tggagatatc tgcttgtttt aacaattctg caatttccag
     6421 catattgccc tgaaacaaag tgaccccaat actcgacgtg gcgtgatact gatgcaccgc
     6481 tatttggtat tgctcattaa gcatcgtttg gattttatgc gccacgactt cggctaagga
     6541 tgccgcttca tttaattgct cgcttaaatt ttcaagcaat acaacaaatt catcgcctcc
     6601 caaacgcgcc actgtatcgc cttcacgcac acttgatgat aagcgctttg caacctcttg
     6661 tagcaacaaa tcccctacat catgtcccag cgtgtcattg agcgctttaa aattatctaa
     6721 atcaattagc agcaacgcac caatttgcct tgtgcgggcg ctagaagcaa tcgcgtgctg
     6781 gagtcggtct ataaacaatt gtcgattcgg taactgcgtc aataaatcat gaaacgccaa
     6841 gtgcagaatt tcatcggccg cggctttacg cgcggtgata tcgacatgcg agccaacata
     6901 atgcgtgacc acaccatcgt tacctttaac cgctgaaatg gagactaagt tataaagtaa
     6961 ttgaccattt ttatggcgat tcagcacctc gccctgccaa gaaccctcac gacgaatttt
     7021 ttgccacatg tctgaataaa aatcggtgtc atgtgtaccc gactgcaata atcgaggcgt
     7081 ttcaccgatc acttcctcag tggtatatcc aaaaatttcc gtaaaggcct tattcacccg
     7141 aagaattcgc aggctagcat cggtaatcat cactgaatct tgtgactcaa atgcaaccgc
     7201 cgaaatttgc aaattaattt ccgcctgctt acgctccgta atatcctgca ctgtgccatg
     7261 caaaccaact atctgaccat tttcgtcatg aatgcctttg ccgcgcgcag tcacccagcg
     7321 tcgagtaccg tccgcacgta ttacttcagc atcacactcg tatggtagcc catctaacaa
     7381 cccctggttc attacaacgg taagttgctg ccaacttttg actgtaaagt attgcggtac
     7441 ttcatcgata tctgccggtg gaagggccag gtcacggcca tatatctcgt atagttcctc
     7501 agaccataca tgcgtattgg tgacggtatc ccaagaccag tcaccgattc tcgcaaaatg
     7561 attcgcttct tttagtctgg cctcactttt ttgcaagcgc tcgttttttt gtttgttatc
     7621 ggtaatatcc agcgagatgc ggcaaatgta ctgaatagcc ccagaagtgt tgagtacggg
     7681 aaaaatcgtt gaggcaaaat agcgtagctc gccagccact ggccaaaact cttcaatggc
     7741 atggcgctgc atggtgcaca gcacaatctc atcattaata cttaaattat tggcaatttc
     7801 ttcagggtaa agatcaaaat ctgttttgcc caatatttct aattccgtca gcccgtgtat
     7861 gcgcaataat tttggatttg ccagcacgta gtgtcctgcg gtatctttca atgaaagaaa
     7921 tgctggtgaa taattcacaa tcgcgcttaa tttaaaatcg cgatcctgca aagctgtttg
     7981 aacctgctta atttctgtaa tgtcgtgcga aatcccaaat aatccaatca cattgcctgc
     8041 atcatcaaac attgggcctt ttgtcacata ggcagtaatt gtgcctgcat ttagtaaact
     8101 cacctgctct tcatgggttt gggttttgcc tttcgccata atttcagcat ccaggcgctt
     8161 tagatgactg gctgtcggtt cagggaaaat aaacgaatca tcttgaccaa taatttcatc
     8221 catcggcttg cccacaatat ctgcacccgc ttggtttatc agcaaatagc ggccagcacg
     8281 gtctttcaca taaatcgcat cacgcgttcc agacactgca acattcagca gcttggcttg
     8341 gttttctgct ttggcccgcg tgcgcatcaa tatttcactt agcacactca ttgtcagtcc
     8401 actgacaata agaaaactcc attgcaataa atcatagcca tcagtaaatt gaaagctcca
     8461 tcgtgtggga acaaacagga gtttggcgcc tattgcaacg gtagctgttg aaataacacc
     8521 aggccaaaat ccacccaata gcgcccctaa gatgattgga aacataaata aaatcatgag
     8581 cggccgatga tctgtattga gccccatatg atgcctaatc aaaagcatca ctaaagaaat
     8641 aagaacagcg aatgaatacg tcagccaagt tggtctgcgg ctgataaaag cacctgaaaa
     8701 tattacgtcc tgcaagtgcc cttgatggtc atttgcaacg cttggcacag tacgcaacac
     8761 aaaaagcaag gctaacgcac tcactaaaac aaaaaacacg cccttttccg ttgagtacca
     8821 atgaattgag tcaatcgtgg taaaggttga taacaggtga tcggataaaa aaacccacag
     8881 caatgccagc acaacgtagg acaacacggt ggctaaaatg aacctttcac gtaacttacg
     8941 catcaccggc ctaattgaga gaaatgaaaa tgaatattaa cagcaaatag aacatagacc
     9001 aatatttagg caacttacat agcccttcgt attgggcttt gcgttactga acgttcgctt
     9061 ctggctcaaa ccaatttaag cgctcacgca ggcgaacgac ttcacccaca atggtgaggc
     9121 acggcgcttt aatattggct tgcgccacct gttgcgccaa gtcttttagc gaagctgtta
     9181 ctacccgttg ccgttgtgtg gtgccttgct gcaccaccgc tgccggcatc tctggcgaaa
     9241 caccatgcgc aataagttgc tcacagattt gcgccaaccc caccaacccc atgtatatca
     9301 ccaccgtttg acgaggccgc gacatggcta cccagtctaa atccagcgtg ccgtctttta
     9361 gatgcccagt gataaataaa caggcttgtg cataatcccg atgcgtcagc ggaattcccg
     9421 cataactcga cacgccattt gccgcactaa taccaggcac cacttgaaat ggcacttttt
     9481 gctgcatcag cgtttcaatt tcttcaccac cacgaccaaa aataaagggg tcgccgcctt
     9541 tcaggcgtag cacgcgcttg ccttctaacg ccaacttggc cagcaaatcg ttgatttctt
     9601 cttgcggcag ggtatgttga tcgcgcgatt ttcctacata aatcaattca gcatcgcgcc
     9661 gtactaaatc catcacttca gggcttacca atttatcata cacacacaca tcacactgct
     9721 gcatgagacg caacgcccta aaagtgagca aatcagggtc gccagggccg ccaccgacta
     9781 aatagacttc acccatctgt tgagtgtcgg gctggtcagc aatcgccttc tctaacgcta
     9841 accttgctgc cgattcttgg cccgctaaaa tgcgttctac gatggggcct tgcaatatac
     9901 cttcccaaaa aatgcgacgc tcttggcttg tggcaaattt agctttgact ttatctcgaa
     9961 actcccccgc aatggctgca atacggccgt aaccggcggg taacatcgtt tcaattttgg
    10021 tacgaatata acgcgccaac acgggcgcgg tgccctcact ggaaatagca atcacaatcg
    10081 gcgcgcgctc tacaattgag gccatggtaa atgtgcataa agctggcgca tctaccacat
    10141 ttaccggaat attttgcgcc ttggccgcga ctgataccgc ttgattgaca gcctcatcat
    10201 cggttgcggc aatcaccaga cacgccgcat tgatttgctg cggttcaaag tgcgctggta
    10261 tataagtaat tttttgtgcg tccaccaatg cctgcagttg caaacaaatt tcaggcgcaa
    10321 aaagcttaac cgttgcattg gctctaagca gcaacgacac cttgcgcaac gccacctcgc
    10381 caccgccgac aaccacacag aggcgttggg taatattcat aaaaataggc aaggcttgca
    10441 tggcgtgctt aatcagtaga ttttgttaaa aatggttgaa ttatttggca cgcaataatt
    10501 ctttaagcgc ttgtttagcc tcaacatccc cctgtaaggc agaaagccga aaccaataaa
    10561 aggcttcttt tctattgcgc tcgacaccgc ggccttcgtt gtacatatta cctaggttat
    10621 attgcgcatc cacatcatgt tggtcggcgg ctaacaaata ccaattcagc gcttgctgat
    10681 aatcttgtgc gacacctgca cccacgtcat atgtatagcc tagcattgat tgtgctgaag
    10741 catagccttg gtcggcagat ttacggtacc agaccaccgc ttgcttttga tcttctttaa
    10801 cgccacggcc ataaaaatac atttgcccga ggttatattc tgctgttgga tttttttccg
    10861 ccgccgcttt gcgataccac atcagcgcct tttcataatc cattttcacc ccttgcccaa
    10921 gctcatacat cacgcccaaa taagtttgcg catccgtatg ccccgctttg gctgactgtt
    10981 caaacgcctt taaggctaag ccgtaatttg ctttttcata cgcacttaca ccttcgtcat
    11041 aatcggctaa ggccgaaaat gaaaaaaaca gcatgagcca tagcatcgtc accaatctaa
    11101 acattttaga atcctcccga atttcacgct aggcgttgtt aaaagtaagt tgttagaagt
    11161 aaggtttcga ttttaaagga ttaagtcatt tgggcaagaa aaaaatcttt ttcaggttgc
    11221 aaaatcactc gagaaaaaaa taatgccctg gaagccttta tttacaaggc tttcagggca
    11281 gtcaaaaagg ttgagtatta attagcgcta aattagcata aatttaagcg ttaatttagt
    11341 gtgatttttt caccgctctc tgcatgaaaa taagttaaat gcgcaaagct gctgccacct
    11401 gaagtgagcg aaccgtcaaa caacgatttt ccgtcaacat ccacaaccgc ataaccatta
    11461 tgatagagcg tcacttccgc taatttaggc gtggcggcct cggctttgtc agttgcctgc
    11521 aatttgccag taggttttgt ccccccagtt tttgtcatag cgtgctgact ttcacgcatt
    11581 tcaaaactaa tgcaattttc ttcttccgcc tcagaataga cttcccactg gcggccgccc
    11641 gtcaactgcg tgagccaagc aggcaaatcc acttctacgc ggcaaataat cggttcatcc
    11701 cacgctgggt gatgaagctc tgcctgcgtg agctcaattg cgttatgttg attggaattt
    11761 tctgtcatgt tatttccttc ttgccattat aatacttcat attgacgatg gtggcggctt
    11821 ggccattttt caagtgcact gattttcttt aaaaacgctt agcagccata actacttaac
    11881 ggctcgtgaa tgcctgaagt ttacagtgtg cgacaaataa tgccaaaata aactattaat
    11941 aataagggga ttttatgttg agtggtttga gcgaaatttc aacgctactt gcgcaaaaca
    12001 gcacgttatt tattaccatt tctgtgatat ttggtttgct ggtgggcagt tttttaaatg
    12061 tcgtcattta tcgcatgccc aaaattatgg agcgagaatg gcacaataac tgccttgaac
    12121 ttcaggggct agaaattccc gagcaaccaa aattcagcat ttcaaaaccg cgttctgctt
    12181 gcccgcattg cggccacaaa attactgcgc ttgaaaacat tcccgtgatt agctacttgt
    12241 ttttaagagg aaaatgcagc agctgccaag ctaaaatcag cccgcgctac ccactgattg
    12301 aagcccttac tggcttgctc atcgggctgg tcagctggaa atttggctac agcagtctga
    12361 cgctttttgc ctggcttttc acgttcgcgc tcattgcatt aacgtttatt gactttgata
    12421 cacaattatt gccagacgac attacgttgc cgttattatg gttaggctta tttgtaaatc
    12481 taaacagtgg atttactgac ttaaaatcag ctgtcatcgg cgccattgcc ggctatttga
    12541 ttctttggtc aatttattgg atattcaaac tggtcaccgg caaagaaggc atgggctatg
    12601 gtgactttaa actactggcc gcgattggcg catggtttgg ctggcaatta cttcctgcgg
    12661 tgattttatt atcgtccatt gtcggcgtta tcattggtgt tgggctaatc atttacgcca
    12721 aacgcggccg tgaagtaccc atgccatttg ggccattttt agcgcttggc ggtattgcgg
    12781 ccttgtttta tggccaacaa ctcgctggtt attatctgca ctaatgtcaa ttattgcact
    12841 cacaggcgga ctgggttctg gcaaaagcga ggcggcaaaa cagtttgcca aacttggcgt
    12901 gcctgttgta gataccgatg tgattgcgca tgcactcaca gccaatggcg cacctgcact
    12961 taagcaaatt agcgaacttt ttgggcctca gtttttaacg aatgaaggtg cattagatcg
    13021 cgcaaagcta cgcgcacatg tgtttgccca cccacaagag cgcagcaagc ttgaagcctt
    13081 attacacccg ctgattcgcg ctgccgcact caagcaactg gccgaaaatg aacatcaaca
    13141 acaccctgac tatcaaattt tggtgattcc gcttttattt gaaaacaatc agtaccaaac
    13201 cagcattcac aaaattttac tgattgattg tgatgaacaa ctgcaaattg cgcgtgccat
    13261 ggcacgaagc tttttgaccc aagccgaagc gcaggccata ttagatgcgc aggtttctcg
    13321 tacaacccga ttaaaacttg cggatgaagt catagaaaac aatggcacat tggccgaatt
    13381 aactgaaagt gtcgagaaaa tgcaccaaaa gctcattaaa atttgccatt aaaccttgca
    13441 tagtcagcca ataactttga taattcagtt caaatttgaa tttaagcttt attttgggtt
    13501 agtgatgtaa accatgccta gttacgacta tccattcaac gaacgtatac gcactttact
    13561 gcggattgaa gacttgttcg ccaaagtaat gcacaatcta gaaacagacc acgaattcca
    13621 ccatcacgct gcgctactca ccttgttgca aatttcagat attgttgaac gcgctgattt
    13681 acgttcagaa ttattgcaag aacttgcccg ccaaaaactg gtaatggatt cccttaaagg
    13741 caacccagcc attgctagcg ccaagctcga aagcattttg gctgaaattg acacggccgt
    13801 tgacgcactt cgtgctgaaa acacaaaact aggccaaaca ctgcgcaaca atgagtggct
    13861 gatggcgatt aaacagcgca ccggcattcc tggcggcgta tgtgagtttg atgtgccgtc
    13921 atatcactac tggttaaatc tcggcccgac aaaacgccga aaagatattg ttgcttggct
    13981 aaacccacta ttgccgatgc agcaagccat aacgattatt ctgcatattc tgcgcggcag
    14041 cggggcaaaa accaagttgc ttgccaaagc aggcgtttac caagaaatgc tttctggcgc
    14101 caaacccgct caaatgctga caatagaaat tgcagacaat acgccttgtt tcccagaagt
    14161 gagtgctaac aaatatgcca tgaatattcg gtttattcac ttagataacg cacagaaaac
    14221 gcaaaaatat gagggggatg ttaaatttac actgaccctt tgcaacttat aaaactttgc
    14281 agcctcatac tcacatcgtt tttttcaaac ctaatttacc cacacattct gtcgacttaa
    14341 aactttatgt ctaccatcaa aaaacgttta gtcacttgcc caaactgcaa aaaactaagc
    14401 gaatactccc ttgagaatgc ttttagacct ttttgttcag agcgttgcaa aatgattgac
    14461 ttgggcgtat gggcaacaga gggttatgcc atacctgttg ctatcaacgc tgcagactta
    14521 tccgacgatg aaaccaacgc cttgctaggg cattaagggg caatcatatg caattattga
    14581 ctttattttc gcgcctctgc acgacaacgc ttctgccaac tgtgctgatg actgtttttt
    14641 ttacagcaca tgccgaaact gcaaaacccg tacaaataca gcaagcttgg gcgcgcgcta
    14701 cagcacccgg gcagaacgtt ggggcagctt atatgacctt aacacatcat gagaacatca
    14761 cgctacttgc cgcagattca gatgcggctg gaaccgttga aatacacagt atggaaatga
    14821 aaaatggtgt aatgaaaatg cggatgcttg aagatttacc ccttaaagcg ggaaaacctg
    14881 tgaagctcgg ccctggcggc ttacatttaa tgttgtttga tttgcaaaaa ccactcaaag
    14941 tgggtgaaaa aattgatcta acactttgct ttaaagatca acgaggcgaa gtgacgcatc
    15001 aaactgtgac attacctatt aaagaggcag cgcagtaacc ccaactattc acggctggta
    15061 ttaccacgcc ccccgttgca tggcaatgcc atgtgcacca tgctgcactg cagtcttttt
    15121 atcatctatt ctaacgccgc ctaacgcata cacgggcaaa ggataatctg caatcatttg
    15181 cccccaagcc tgccagccta atggtatggc atcgctgtga cttaacgtcg cgtttactgg
    15241 cgaaagcact acaaaatcca gccctagcgt ggctgctttt tcgagttctg caagattgtg
    15301 gcaagaggct gcgcaaagca tatcatccgg cctttgcctt aattcaagca attgatgggc
    15361 tggcaaatgc acgccgtcag cgcccaactc tctcaccaat tcagcattgg cattcagcag
    15421 cactttcgcg ctgcgtgcgt gtgccaagtg gatgacgcgc tcgctaaaag tttgtaacgc
    15481 tgccggttgc agttgctttt cacgtatttg aatcagtttt aagccatttg ctaacgccaa
    15541 ttgcaactgg ttaaaaaatg cttgttcacc catttctgct aggttggtga tggcataaac
    15601 atcaggtaaa ttaagtgcct gcataatcgg ctgatttgcc ggcaacatgg gctgcacgcg
    15661 cacctgactg ggctgttgcc agcttagttg ttgaccctca cgcccatgcg gctcgcccaa
    15721 ccaagccaat actctaaaaa aatgtaattt gacggttttt tcagggtagg catatacgcg
    15781 cgtaagccaa ggtgccattt ccgttactgt aatgcctagc tcttcatgta gttcacgcat
    15841 taaggcgcgg gcgggcgttt cattttcttc aattttaccg cccggaaact cccagtagcc
    15901 ggcccaaggt ttacccgcag gccgctctcc taataacaca gcaccatctt gccgttgaat
    15961 tacccccacg gctgcatgtg ttaccgcagc agacggcgaa actgaatcaa gatttgttgt
    16021 catgctgact ttattggcgt aaggtcgaca aggcgagctg cttgataccc gcgtaatcgc
    16081 gcgcaaattg ataggccacg cgaccgctac gcgctccgcg tgtatgggtg aactttaatg
    16141 cggcctcgtg tgcggcggca tcaaattcaa aaccaaaatg cttaagccaa gtggcgcaaa
    16201 ttgccaaata ttcctcttgg tcaaacgcat aaaaagacag ccataaacca aagcgctcgg
    16261 ccagcgcggt tttttcttca atcgtgtcac tcggccgaat ttcaccatcc caatgcttgg
    16321 tagctagatt atctgccata aattctggca tcaaattttt acgatttgac gtggcataca
    16381 ccagcatatt gtccgatgca ctggcaacgg aaccatccag caccactttc agcgccttat
    16441 aactttcatc attggcatca aaagtcaggt catcgcaaaa aataatgaat ttttctgggc
    16501 gattgcgaat aatctcaaca atttcaggca aatgcactaa atcgtgcttt tccacttcga
    16561 tcaaacgcaa accttcggcc gcataagcgg ttaacagcgc cttaatgagt gaagatttac
    16621 ccgtcccgcg cgcacctgtc agcagcacat tattggcagt aaagccttgt aaaaattgct
    16681 gtgtattgcg tacgatttgt gttttttgcg catctacaaa ataaatatct gcgagcgcta
    16741 tgtggtgtgg atgcgcgact gcctgcaatc tgccttgatt gttttgctta acccagcgcc
    16801 atgcgaatgc ctgccaatca accggtgata ttttggctgg caacaggttt tctagctgat
    16861 taattaaacc ttcagcgcgc tcaattaaat gcgtgagttt atccattaag agcgataatc
    16921 tgcgttaatt ttgacgtaat cgtacgaaaa atcacaagtc caaaccgtac tttgcgcaga
    16981 accgcgcgct aaatccacac gcactagaat ttcagctttt tgcatgattg cagcgccctg
    17041 cgcctcttgg taagccggtg cgcggccacc atgttcggcc accagcacat cgcccaaata
    17101 gagttttaac gcattcacgt ctaacccttc aaccccagca tagccgatgg ccgccaaaat
    17161 tcggcctaaa ttcgggtctg aggcaaaaaa agccgtttta attaagggtg aatgtgcaat
    17221 tgcatacgcc actttgcggc attcagccgc atctcgcccg ccttctacct gcacagtcat
    17281 gaattttgtt gcgccttcgc cgtcgcgcac aatcgcttgt gcgagttcaa ttgcaagttc
    17341 ggtaaacgca tcacgaagca cggcaaattc tgggctggtt tcagtaattt cagcattgcc
    17401 tgccaagtgc gtggccatca aaatcaggct gtcgttggtg gaggtgtcgc catccactgt
    17461 aatgcagtta aacgatttat tcacggcata ctgaatgatg ctttcaagcg cagattggct
    17521 tacagcggca tccgtcgcaa tatagccgag catggttgcc atgttggggt gtatcatgcc
    17581 gctgcctttg ctgatgccgg tgagcgtgac ggttttgcca cccaactgta tttggcgtga
    17641 cgccccttta gccacaatat cggtggtcat aattgcctct gccgcgttta accagttatc
    17701 ttctttcaaa ttggcaatgg cggcaggcaa gccagcgatg actttatcta caggcagcgg
    17761 ctctaaaatc accccagttg aaaatggcaa aatttgattc gcttgcaagt tcaacaaatc
    17821 ccccaaagct tggcaacttt gttgtgcgcg atttaaacca tcttcgcccg tgcctgcatt
    17881 agcacagccc gtatttacca acagcgcacg aatgcctgct ttttgcttta aaaattcttt
    17941 acacaacagc acgggcgccg cgcaaaattg gttcagcgta aacacacccg ccacatggct
    18001 actttcagcc aaggtaacaa ctaacacatc tttgcggttg ggcttgcgca catgcgcttc
    18061 tgcacagccc aacttaaccc ctttaacggg aaataaagcg ctggcttgag gggcagtaag
    18121 attaactggc atggtgaaaa atccgtgtaa aaatgttgtg cttattttag cgcattattg
    18181 attgagcaag cagatgtgac ggcctatttt taaggcaaat catgctagtt taaaagccat
    18241 caattcgtat gatatttcac ccgagaaaat atttatgccc tgcaagcctt tattcataaa
    18301 gctcacaggg catcgaaaaa ggttgtgtta aatttaagat gaatttaact gttttttgcc
    18361 tacgtttaac taataacgtt tcacattgct gaccttgcca gtcagatcaa aagtggactg
    18421 aaagttacgc ccttgtgcaa ctgcattgag gctgattttt cctgatatcg cttcactagg
    18481 ggcaatttgc acttggcctt gcgcttgcaa ttttggcgat ttcagcacca aattgcgata
    18541 ttcaacccgg ccgctttttg cccacacacc gcctgtgagt tggtcaaaat tggtggcata
    18601 gccttcaagt gctttatctt cagggttaag tatggcgcgc gccaaatcaa cgccattaat
    18661 tttaccgttg ggtatattaa agtttcctgt cacttcggca tcgtctagca attgagttgc
    18721 gatatttgac tgggctgaaa atttgcctgc caagtttaac ttaccatcca ccaaagctgt
    18781 gctggaaaaa gcttttaaca gctgggcggc attggctttt atgagattaa aattacccga
    18841 tgtttgccac tgattggacc aatctaccat tgcttttgcg ctgaatgttc caccataaat
    18901 ttcaccctgc aattggtcaa gttgtaggcg ctcctgctga tacgtgccgc tggcatgaag
    18961 cgcttcaaat acgatgtttg gtcgtactgg taacgtccac ttgcttgccg caatcttcac
    19021 ccgatattga tttgcttgcg gttgcacctc aatggctaac gcgccatctt cactttttaa
    19081 cgcaatgccg ccgagttcgc ccgcatgatg ttgcatcaca tggccattaa aaggcaccaa
    19141 agtaagatcg cgaatattaa gttttacttt gctaaagcgc acatcttcca cctgcagttg
    19201 cggtgactgc cttgccgctt gtagccactg taacggcttg gcaacgtctt tctgattcag
    19261 cgtaacgcct tcaattagca gactgtgaag cgttttaacc tcactcaaaa gactggtaat
    19321 ttgcggcgcc gctttgattg aatccagttg aatggcagga ttttcaccca tcattacatg
    19381 gtgcaacata aaatgcggct gcggccaaag ggaggcgcgc acttcgccaa ctttaactgg
    19441 ggcgccaata ctggctgcgg caaagttttc tatcggcgca accagataat taagtggtac
    19501 aaaatgcatc agacccaaca ataaaatgag tgctattggc atataaacca atgaaaatcg
    19561 caacaggcct gacaaccatt ttttgggttg ctttgcattg tcatttgcac ctttaaagcg
    19621 gcttaacacc aaaatatcaa attttcgata cttggcttta gccacgttca acaaactgtt
    19681 ttttgcacgt agcggcttgt ggcccatcat ttcgctcgcc aagcttttgg cttcttcgcg
    19741 tgctgctttt tcagcggctt gttcttcggc ttttactgcg gcctgtttca cttctttttc
    19801 ttttttagca aggcgttcag cctctttctg cgctttagct tcggcttttt tctgtgtctc
    19861 tctctcggtt ttttcttttg caatacgcgc tttttctaac ttagcctgtt ctttggccgc
    19921 tgctttttcc tttgcattgg cttccgcaat cagtcttgct tggcgttcag cttcaatttt
    19981 ggcttctttt tctgccaaag cacgggcgtc agcttctgct ttcttctgtg cggcttcggc
    20041 ttcgcgtgcc atgcgctctg cgtctaaacg tgcttgcgct tctgccgcta atctggcggc
    20101 ttcttctgcc gccgcgattg cttgtaagcg ctcttcctcc tgctttgctg caagttcggc
    20161 ttgcgcttgc gcttcctgcg ccagtttttc ctgacgttct gcctcaagcc gtgctgctgc
    20221 ttcagcctgc gcttgagctt cttcagccaa tttcgcctga tgctctgctt caagccgcgc
    20281 ggcttcctca gcttgtgctt gggcttcaag ttccgcttgt aggcgcgcct cctctgcggc
    20341 ctgtgcaata cgcgcagctt ctaggtgcgc ttgttcttct gcggctactc tagccacttc
    20401 ttccgcctcg gcaattctct gcaagcgttc tgcctcttgc tttgccgcaa gctcggcttg
    20461 caatcgggct tcttctgcca atttcgcctg atgctcggct tcaagccgcg cagcttcttc
    20521 agctttagca agcgcttcgg cggtggcttg ttgttcttgc gcctttttct ctccggctaa
    20581 tctatcagcc tcttcagcct gcgctttggc ttgaagtgct aacgccactt gcatgcggac
    20641 ctcttcggcc tctttcattt tgcgggtttc ttcacggcgc tggcgttctt tttctcgaat
    20701 ccgctcaact aaagcttgtg ctgcgtgctc tgctttttgc cgcgcagcat gttctgcttg
    20761 ttcacgcgct tgggcctttt tctctgcaat gcgcgctgct gctaatcgcg catcttcctc
    20821 cgcagcacgt ttggcgtttt ctttggcctc ggctatcgcg cgttcctttt cagccgtttc
    20881 aagcacgacc ttttcagctt gcgcacgcgc ctctgctgct aacctttctt gtcgctcatt
    20941 ttctaatttc gctttggctt gcgcggcttc cgcttcagca cgaatacgcg caagctctgc
    21001 ttcacgcgct aaatgctccg cctcaagtcg cgccttttcc tgcacatcta ctttagcctg
    21061 tgccttggct ttcatttcag cttgtttgcg cgtttctgcc tctgcttttt gtcgctcaat
    21121 ttcagcttgc tgcgctcggt gcgcttcttc ttcagcatct ttctggcgga tttttacttc
    21181 ggctttggca atttcttttg cgcggatttg cgcttcaact tcacgcgctt ttagttcatc
    21241 ttcacgccgc tctgcttctt tctttttagc aatttgctca agttctaaat aggtttctaa
    21301 ctcctctgtg ccgtcaattt cctcaacgtt gtacacctct tccaccacca tcggtgagaa
    21361 taaagtatcc aactcccaat catcttcttg tgcgacgact tcaacaattt taatgtagcc
    21421 ttcattttca atttgggaga tggcagctat gaatttttgc tcagaaaaac cctcgagctg
    21481 atttaaaata ttttgcgcgg aagttttgcc atcaatcagc gccataattt tcattatatg
    21541 tgcaggcaaa ccgccaaaaa gggtttttcg aaggcgtaca cctttgggtg ttttagcgta
    21601 aacaactgat aagtccatcg ctaccgttac ctaatttata tatgttggcg tgacttggtc
    21661 acaatggcat tccaacataa aagagtaaat gccaattcaa taaaaaacca gctaaaaacg
    21721 cgcctgttat aggaatttaa ggacgcaaaa tgtagtgaaa actcagcatg ttattgagaa
    21781 aagtctgatt ctaacaacat tttaatagat gctggaattt taacgcaata ataaaaactc
    21841 aatgcgccaa gattgttaaa aagcattaag ttaaaatggc tgaaaaatgc cgagaaattt
    21901 tactgccctg cgagccttta tttataaggc ttacagggca tcaaaaaagg ttgcatgaaa
    21961 aatacgcttt aaaaaaggta cttttacttg atgcaaaatt gactttgcta cattattttt
    22021 ttttcagcat caattttagc gcgggccaaa tattttttaa tatcacaggt tggccaagcg
    22081 cgttggggtg caggccatcg gcttgaacca aatccggctt tgccgcaact tggtctaaca
    22141 taaaaggcac aaggctgatt ttgtgttgct gaccaagttg ctgatacgtc tgaaaaaagg
    22201 cttcggtata tttgggcccg taattgggcg ggatacgcat gccaagcaat aacactttgg
    22261 ctttattttt ttggctgtaa gcaatcatcg cactcagatt tttgtgcatt tcttgaatcg
    22321 gcaaaccgcg taagccatca ttggcgccaa gggctaaaat gacaatcatt ggcctggttg
    22381 ttgtaatcgt tttttcaaag cgagtgagtc cgccactggt ggtttcaccg ctgatgctgg
    22441 catttgcaac ctcataatga tacccttcgg cattgagctt ttgttgcatt aatgtcaccc
    22501 agccctgcgg ttgcgcaatg ccgtaagccg cagataagct atcgccatat accaatattt
    22561 tgctgttttc ggctaaagcg tgtgtactta atgcaacgaa aatgaaaccg aacagactaa
    22621 ggttgagtcg ttttacaaaa cgactgctga atacatactc aaatatcaat aataagtgga
    22681 aaaacattaa tgcaatcatc tcctattgtg caagcgcaag ccttaactta tgaatttgtt
    22741 agccatgata accatattcg cgtcttatat gatttaaatt tatctattgc ggctggcgag
    22801 caagtcgcca ttattggcag ttcaggctca ggtaaatcca ccctcttgag cttactcgcg
    22861 gggttggaca tccccaccag tggcagcgtt caaattgatg gcaagtcgtt tagtcagtta
    22921 gatgaagatg gtcgcgcagc catccgcgca aagcgtatgg gttttgtgtt tcaatcgttt
    22981 cagttgctgc cttcactgac tgcactcgaa aatgttatgt tgccgctcca attaaaaaat
    23041 catcctgacg cagcatcgtt gagcttagct gcgcttcgtc aggtgggcct gagcgagcgc
    23101 gtacagcatt atccaaaaca actttctggc ggtgaacaac agcgcgttgc catcgcccga
    23161 gcatttgcca cccaacctgc cattttattt gccgacgagc cgacaggcaa cctcgaccac
    23221 aacactgggc agcgtattat cgaattactg tttgaaatga acgcaaatgc aggcaccaca
    23281 ttggtattag tgacgcacga cattcagtta gccaaccgct gccacacaca gcttcaatta
    23341 gcgcatggca cgctcacgag gttgaatcaa ccatgagtgt gactttgaaa ttagcttggt
    23401 cacaaacgat gagtttgtgg aaatcaggtg cattgaatgt gttggttttt gccttggtac
    23461 tcgcggttac ggccattact gccaccggtt tttttacgca gcgcatcgaa accgcgctga
    23521 atcagcaggg tggcttgctg ctgggtggcg accttgcggt gctggctgac catgaaattc
    23581 cagaaacttt catcaacaac gccaaaactc agggtattac tacggtaaaa acttatgaat
    23641 tccccagcat ggtggtgttt ggtgaagcca atcagttggc cgaaattaaa gcagttgaag
    23701 ccggctttcc gttacgggga gacctcacta ttggtcaaca cgccaccgat accggaaaaa
    23761 tcgtcaacca aggcccaaac actggtgaag tgtggataga accaagactg gtaaatctgc
    23821 tgaatattca ggtgggtgat aggttggaag taggcgagcg ccaattacgt gtaagtgcca
    23881 ttttagtgcg tgaaccctct cgtggtggcg atatgtttgg ctttgcaccg cgcctgatga
    23941 tgcacgccgc agatttaccc tctaccaaac tcattcaata cggtagccga gtgaaatatc
    24001 aattattact cgcaggccaa ccgcaaagca tttcacagta ttttgaacaa atgaaaccac
    24061 agttgaatcg cggtgaaaaa attcaggaca ttcgaaatgc acggcctgaa attaaatctg
    24121 ccctcgacaa agcgcaacag tttttaggtt tatcgtcaat ggtcagtgtt attctcgcca
    24181 tggtggcaat gctgctctca agcctgcctt acatcaaaca aagtcttgat acatttgcac
    24241 tgatgcgctg ttttggtgcc tcaaaaaata atgtgttaca agtattggca tttcaaacaa
    24301 cgataatcgc cctacttagc gcactattcg gcgccgcgct tggttacatt gggcaactcg
    24361 gcttagccac attagcgggc aacttgtttg tagaaacgtt acctgcggcc aacgccacac
    24421 cgctcttttt gggctttctg gcaagccttg gcatgatgtt ggcggtggtg ctgccccatg
    24481 cctggcaaat gcgcaatctc acagccatga atattctaag gcgtgaaact atcagccagc
    24541 ccttatcgac acaagccaaa tttttgccag ctttctttgt aatgaccctg atgatttttt
    24601 ggcaggcacg agacattaaa cttgcactct caacgattat tgcactcacc ctgctgtgca
    24661 tgatcattgc cggcattgct tacctacttg tcagcttagt cagtcgtgtg ttccggttgt
    24721 catcacgcaa ccaaatgctg ggccaaatta aaatcggcat tttgggcatc aagcgccgtt
    24781 ttggtttatc cactgtacaa atgataggct ttagcatggg cctaatggtg ctaatgctac
    24841 tggctttaat tcgcaatgat ttaattcgta attggcaggc ctctctgcca attgatgcgc
    24901 ccaatcggtt tgtgatcaac attcagcctg cgcagattag cggtataaag gaatttttta
    24961 cccagcaaaa aattcaagat gcaacgattt tcccgatggt gcgcggccgc ttaatcagcc
    25021 aaaataataa agatctcagc caaaccgaat ggaaagatga gcgtgcgaaa cgcctagcag
    25081 aacgagaatt taatctctcg tgggcagcca acatgcaaag tgacaacaaa ctgctttcgg
    25141 gcaggtggtg gacagaacaa gaccacggaa agccttacct gtcacttgaa gaagacttgg
    25201 ctaaaacatt aaacctaaaa ctgggtgatc aattagtttt taatgtggcg ggaaccccac
    25261 ttaacttaac cgtcaccagt attcgaaaag tcgaatggga caccatgcgc ccaaactttt
    25321 ttgcggtgac tccacctggt gtgttagacc aatatcctac cagttacatg agtagctttt
    25381 atttgccttt aagtgcagat gcacccatga accaactcat taaaacctac ccaaatctta
    25441 cagtgattga tgtcgcggcc ctcatgcaac aggtacgcgg tattatgcaa aaaatgagca
    25501 gtgcgattga atacgtgttc gcctttagtt taatggctgg catcgcagtg ctatacgctg
    25561 cgctgatcgc aaccagagat gaacgtgtga cagaggccac tttaatgcgt gtttttggtg
    25621 cgtcacgtca gcaggttagt gtggcctaca tagctgagtt tgcctgcatt ggcttaattg
    25681 cagcgctggt ggctgccatt gccgccaatc tactggccta ttacctaagc cacttcgtgc
    25741 tggatattcc gtttcagctc aatttcacct tagcggttac tgtcatgctc attgctgcca
    25801 tctgtattcc gctcgccgca tggctggtgc taagaaaatt tttaaacgta cctgccagac
    25861 agctcctgaa cagcatttaa gtattagcta atttacgcac aaaaaaagca cgcctaagcg
    25921 tgcttcttaa ttagataaag caactaattt agacttattt tgcttctggt gcagcttcta
    25981 ctgctggcgc ttctgcaggc gcactcgctt cagtcaccgg ttgcgctgaa tcatcaaggg
    26041 taatgccagg cacgcgctct tcaggtgttg gctcatagcc acctggctca gctgcagctg
    26101 gtgctggtgc tggtgctgct tcaacaggcg ccgcttctac tggcgctggc gcttcaacgg
    26161 ctggcgcagc ctccactggt gcagcttctt tttcaccaca acccacgagt aataaactgg
    26221 taacgcttag tgctaataaa gcacgtaata atgtagacat tatgattcct ttcaacttgt
    26281 tgataaacat aaaaaaacct tgaactattg acaagtaaca aggatttttt tatctgacta
    26341 aactcgaagt tgcattctag cgatatttct catgaa
//
LOCUS       DAOQSI010000010        26126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_1158_length_26126_cov_5.189866, whole genome shotgun sequence.
ACCESSION   DAOQSI010000010 DAOQSI010000000
VERSION     DAOQSI010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 26126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26126
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1158_length_26126_cov_5.189866"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2289)
                     /gene="acnB"
                     /locus_tag="PKL42_01555"
     CDS             complement(<1..2289)
                     /gene="acnB"
                     /locus_tag="PKL42_01555"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HNU65784.1"
                     /translation="MLKAYTAHVQERATQQLPPLPLNAEQVALVVALLKNPPAGEGEI
                     LLDLIANRTPAGVDQAAYVKAAFLADIANGKASSPLISAEKAVALLGTMLGGYNVQAL
                     VALLDLSDSNPTLAEHAVKALSSTILMFDAFHDVDVKMKAGNAHAKKLIESWANAEWF
                     TNAPALAQELKMVVFKVDGETNTDDLSPAQDAWSRPDIPLHAKAMLINKMPDGLKRID
                     ALKQKGLPLAYVGDVVGTGSSRKSAINSVQWFMGNDIPHIPNKRTGGVILGGKIAPIF
                     FNTAEDSGALPIQCDVSKMATGDVITIQPYAGKVLNEAGEIIATFTLNPVTMPDEVRA
                     GGRIPLIIGRGLTANARKALGLNDTDIFIKATPEVTSSKGYTLAQKMVGRACGLPEGV
                     GVRPNTYCEPIATTVGSQDTTGGMTRDELKELACLGFSSDLVMQSFCHTAAYPKPVDI
                     KLQHELPEFISSRGGVSLRPGDGVIHSWLNRMILPDTVGTGGDSHTRFPIGISFPAGS
                     GLVAFAAATGAMPLDMPESVLVRFKGTMQPGITLRDLVNAIPYAALQEGSLTVGKTGK
                     KNVFNGRILEIEGLPDLKVEQAFEFADASAERSANGCTVLLNNAPVIEFLNSNIVLLQ
                     NMIDNGYQDARTLQRRIENMQAWLAKPELLTPDTDAQYAHVIEIDLNTIKEPLVACPN
                     DPDDIKPLSAVVGDKIDEVFIGSCMTNIGHYRAAAKVLEGSNNIPTRLWIAPPTRMDE
                     AQLRDEGVYSTFGVAGARTEIPG"
     gene            complement(2388..2705)
                     /locus_tag="PKL42_01560"
     CDS             complement(2388..2705)
                     /locus_tag="PKL42_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65785.1"
                     /translation="MHTQRRFIKTIFAATLLAMATLSHAASVLDYLTDANGKYTGPTV
                     EDNIALMDKDKNGFADASEVRAYLELINGKGYQSAVLDKFEASMRGTSCNTPFAKDFY
                     VNN"
     gene            complement(2777..4279)
                     /locus_tag="PKL42_01565"
     CDS             complement(2777..4279)
                     /locus_tag="PKL42_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNU65786.1"
                     /translation="MTTIKQSDFIQSIKDALQYISYYHPADYIQALGRAYEAEQSPAA
                     KDAIAQILTNSRMCAEGKRPLCQDTGIVVAFVKVGMCVQWDAILDIEQMVNEGVRQAY
                     LLPENKLRASIVSDPAGKRANTKDNTPAVVHVSLVAGDQVEVQIAAKGGGSENKAKLA
                     MLNPNESIVDWILEVVPQMGAGWCPPGMLGIGIGGSAEKAVLLAKESLMAPIDMHELK
                     AKGATNRLEELRLEIFEKVNNLGIGAQGLGGLTTVLDVKILDYPTHAASLPVAIIPNC
                     AATRHVHFVLDGSGVAELTPPDLSMYPAVHWQPSAASKRVNLDTITKTETATWQAGET
                     LLLSGKLLTGRDAAHKRMADMMAKGEPLPVSLQNRFIYYVGPVDAVRDEAVGPAGPTT
                     ATRMDSYTELMLSQTGLIGMVGKAERGEEAIEAIKKHQAVYLTAVGGAAYLVSKAIKK
                     SRVLAFEDLGMEAIYEFDIVDMPVTVAVTSRGESVHQTGPALWANILLKH"
     gene            complement(4488..6146)
                     /locus_tag="PKL42_01570"
     CDS             complement(4488..6146)
                     /locus_tag="PKL42_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HNU65787.1"
                     /translation="MFTKFPEWQWIIPKAFNIGVACTDRHLGTPQASNIAMIVEDDVL
                     GTSQITFNELSRKTDQFAQVLRNLNVQVGDRVLIRLPNSLDYPIAFLGAMKMGAISVP
                     TSTLLTAEEVAYLAKDSGATVLVTDAAAWEVMAGEIENALGQSPTLKHVLLSQAIETK
                     PHSTFNVLNLAASLHETQECEAPYKTQANDPAYLVYTSGTTGYPKGVLHAHRALLGRQ
                     PAAQYWFNYSDAADANGSQKQDRIMHSGKFNWTYVLGTGLMDPLYLGKTVIVHEGKND
                     AQKWLDLIQKHAATIFIGVPTIYRQILQKTTTTGAQLPSLRHYMSAGEHLSDEVLSQW
                     RERFGLEIYEAVGMSEFSYYLSQSKFRPIRAGSAGFPQPGHDIQLLNPETLEAVETGE
                     EGMICVPDNDPGLFLCYWNLDDETAKYKHDGYFFTGDYAKYDADGYIWFLGRKDDIIK
                     SFGYRVSPYEIERVYKGHPDVADCAAVGEEIEKDKLLVVIYVILKPEASTSPDELLGY
                     GKQHLAAYKTPKTVYLAQDFPRTKNGKILRKDISPKIAVAKSSR"
     gene            complement(6289..7419)
                     /locus_tag="PKL42_01575"
     CDS             complement(6289..7419)
                     /locus_tag="PKL42_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018984989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HNU65788.1"
                     /translation="MMMHIGEQALSLDASALGDYWPGIQLYYPPVKYAPSLGIYEDMA
                     QASARMKKHAHFTSAHTLIFDLEDGCRQKVMSRDLLRQELPMLKKMNPRVTVAIRCNS
                     FRTDEYELDMKLVCKMGEYIDVVMLAKAGEAYGAAEVRDLSSFLLSVNPNITIQPIIE
                     HPKSLKIAPELMQYNTVKHVVFGIHDFSKAMGIHITPENWIEELQFYMNQLMFEARIA
                     GKGVIGGVETLIGASAMPEKFLEPHDVRRWLDLHGDNESRVVYKHACQEAAMGLTGKQ
                     VIHPAHIHLCKTAYTPSPTDVAQKVAILKAAIEADALHGGAIKFEGEMLDPPMFGKAL
                     QTLLRAHALRALKTSDMDFAVHVLQLLPAQVVRQNWPYGVLL"
     gene            complement(7421..7705)
                     /locus_tag="PKL42_01580"
     CDS             complement(7421..7705)
                     /locus_tag="PKL42_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HNU65789.1"
                     /translation="MMNAENILDAEQKRLAWRCRRGMLELDIVLQRFVKAEFNQLNLG
                     ELRVFDAMLELPDNDFWDLLNGSKQPENDAHLTAVIHKIKAVRFNDQEGA"
     gene            complement(7709..8446)
                     /locus_tag="PKL42_01585"
     CDS             complement(7709..8446)
                     /locus_tag="PKL42_01585"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HNU65790.1"
                     /translation="MKFSIYRYNPDVDAKPYMQEFDVALQEEDHMLLDALLRIKELDD
                     TLSLRKSCREGVCGSDAMNINGKNGLACITKLADLEEPIVLRPMPGLPVIRDLIVDMT
                     QFFEHYNSVKPYLINHDPLPETERLQSPEDRAKLDGMYECILCGACTTSCPSFWWNPD
                     KFVGPSGLMQANRFIMDSRDQATEERLENLEDPYRLYRCHNIMNCVEVCPKKLNPNAA
                     IANIKDLKIARAKDKKPAKKIFGIKVG"
     gene            complement(8448..10223)
                     /gene="sdhA"
                     /locus_tag="PKL42_01590"
     CDS             complement(8448..10223)
                     /gene="sdhA"
                     /locus_tag="PKL42_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HNU65791.1"
                     /translation="MSLQIHQFDAVIIGGGGAGLRASLQLAEAGVKVAVLSKVFPTRS
                     HTVAAQGGIAAALGNVSDDKWLWHMYDTIKGSDFLGDQDAIEFMCKNAAKAIIELEHF
                     GMPFDRLASGKIFQRPFGGMTQNFGETPISRTCAVADRTGHAMLHTLYQGNIQANTKF
                     FVEWFALDLIRDADGDVLGVIALEIETGEIHILHAKTTLLATGGAGRIFQASTNAFIN
                     TGDGLGMAARAGLPLQDMEFWQFHPTGVLHAGVLITEGVRGEGGYLVNSEGERFMERY
                     APHAKDLASRDVISRASATEIKEGRGVGKNKDAVYLKLDHLGEELISKRLPGIREIAK
                     TFANVDPIKDPIPVVPTAHYMMGGIPTNLDGQVVLFDEKTGEKTVSGLYAVGECACVS
                     VHGANRLGSNSLLDLVVFGKAAGDKMVQEIAQNKAHKPLPANVADATLARIARLENTQ
                     GENAQGENVSEVGAAMRKTMQTHCGVFRFPELLSEGVVAIDAVAKRAQHITIKDKSQV
                     FNTARIEALELDNLIEVAVATMRTAHARQESRGAHAREDFAERDDTHWLKHSLFFKQN
                     NAITYKPVRLTPLTVESFEPKKRVY"
     gene            complement(10230..10577)
                     /gene="sdhD"
                     /locus_tag="PKL42_01595"
     CDS             complement(10230..10577)
                     /gene="sdhD"
                     /locus_tag="PKL42_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HNU65792.1"
                     /translation="MLIELLTDKYPGMRFWLTQRLTALVMAAYLVLVILLLVIVQPAR
                     FEDWHALVSPVWFRLATILFFASLCMHAWLGVRDVLRDYVFNQGLRGYLQIIVDVLLV
                     IYFVWLCFILWNL"
     gene            complement(10582..10968)
                     /gene="sdhC"
                     /locus_tag="PKL42_01600"
     CDS             complement(10582..10968)
                     /gene="sdhC"
                     /locus_tag="PKL42_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HNU65793.1"
                     /translation="MKKNQQKNRPKNLNLFTIRLPINAVVSILHRASGVALFLVLPLL
                     LLALQASVKSPESYAEVANIFGAWWVKLLLIGLSWAFFHHFFAGIRHLLQDVHWMTSL
                     QKARFSSRVVLWLVGVSVVIFGISIW"
     gene            complement(10989..12182)
                     /locus_tag="PKL42_01605"
     CDS             complement(10989..12182)
                     /locus_tag="PKL42_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HNU65794.1"
                     /translation="MDDLKKRALDYHQFPIPGKLSVESSKPCATQDDLSLAYTPGVAA
                     PVREIHEDPANAYKYTNKGNLVAVITDGTAVLGLGNMGALASKPVMEGKAVLFKRFAG
                     IDVFDIEVNAKTPDDFIETVVNIAPTFGGINLEDIAAPHCFYIENELKKRLDIPVFHD
                     DQHGTAVIVAAALLNALSIQGKTLSKAKIVFLGAGAAGCSCAKLLKLMGATNITMTDR
                     SGVLDTDRADLHDNNRELAVVPSDAKVLADVMPDADVFIGVSAKNALDASLIKSMAKN
                     PIIFALANPDPEVLPSVAHAIRDDVLMATGRSDFPNQVNNALCFPYLFRGALDAKARQ
                     ITEEMKIAAAHALSELAREPVPAEVLQAYNLTELKFGKEYIIPKPFDPRLLARVAGAV
                     AKAAK"
     gene            complement(12422..13156)
                     /locus_tag="PKL42_01610"
     CDS             complement(12422..13156)
                     /locus_tag="PKL42_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNU65795.1"
                     /translation="MDKQSNSPSYRPLYDQIKVLITQSLIAGEWRPSEMIPSEVELAA
                     RYKVSQGTVRKAIDSLAAENILIRRQGKGTFVATHEAEGMKLRFLRLTAVSGQKELLN
                     NEFISCEKSKADAHIAQVLDIKAGAATIEVKRLLTFSGRPLIYDHIIVPAAPFKGLNS
                     AKVEESNGSMYSMYESQFGVRMIRAEERLKAVAASQDVAKALGLKEGYPLLSIERVSF
                     TYGDKPMEWRLGLCLTDDHHYMSELE"
     gene            13347..14228
                     /locus_tag="PKL42_01615"
     CDS             13347..14228
                     /locus_tag="PKL42_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HNU65796.1"
                     /translation="MEYCLRPRRSMLYVPGCNQHYLDRARTLAADSVILDLGDPILID
                     AKLQSRDNVVAAVKQGGYGSREVVVRVNNLDSIWGHDDIKAVANIGADAILFPNIESA
                     EHVHTALELLDAAGGGHMPIMVMIESPLAVLNAKEIASASDRITCIVMATSDLISQLH
                     ARVTHERSAILTSLSLVVLAARAYGRCVIDGITSDFKNMHSFEYACRLGRDMGLDGKS
                     LVHPAQIAYCNDAYTPKASEVANARLIIKALQEANEGGLGTVVVNDKLVEQHHVKAAQ
                     RLLKLSDMITELEEDFI"
     gene            14229..15308
                     /locus_tag="PKL42_01620"
     CDS             14229..15308
                     /locus_tag="PKL42_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNU65797.1"
                     /translation="MTLSTKINAGRFFEDFVLGEVILHATPRTITAGDAALYIALTGS
                     RNPLHCSEPFAQSLGYKSTPIDNLLAFHIAFGKTVPDISVNAVANLGYAEAKFIQPVF
                     VGDTLTTSSEVIGLKQNSNGKNGVVWVRSTTVNQHKVAVMSWVRWVMIHKSEHHQTNL
                     SAPAPETVIPELAPFVSVEHLQLSPYMSAKYYQTNATGGDYLWEDYQIGERINHAAGM
                     TINESDHSLATRLYQNNAKLHFDAHLMKNSAIGQRLVYGGVVISACRALSFDGLENAL
                     SILAINGGTHANPCFAGDTIYTFTEVLDAWPLPKRTDIGALRLRMVGIKNMAAHEIGE
                     IKTENGYHPNVVLDLDYTVHMPRKH"
     gene            15340..16329
                     /locus_tag="PKL42_01625"
     CDS             15340..16329
                     /locus_tag="PKL42_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HNU65798.1"
                     /translation="MATLVHPSEALFGGEKPFPLIPACEHFAGSEKLILKALSLQDTI
                     GPVFDITCDCEDGAASGLEREHAEMIVRVLNSDANQHKMAGARIHDYTHPAWKLDIDI
                     LVAGAGQVLSYITIPKCTDIAQAKEMIAYIQKVATFSGITRVIPVHILIETHGALYQV
                     HEIAKLPWLQVLDFGLMDFVSAHHGAIPALAMRSPGQFEHHLLARAKTEVVAAALANG
                     VVPAHNVTLDLKNVETTLSDATRARNEFGFLRMWSIYPTQIQAIVDAMKPNYDEVIDA
                     ANILIAAQDMAWGPIQYAGELHDRATYRYFWEVLQKAKLTNVTIPDEAQKRFF"
     gene            16329..17216
                     /gene="prpB"
                     /locus_tag="PKL42_01630"
     CDS             16329..17216
                     /gene="prpB"
                     /locus_tag="PKL42_01630"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HNU65799.1"
                     /translation="MSNLITAGARFRAAIAQEKPLQIIGAINAYHAMLATQTGFKAIY
                     LSGGGVAAGSLGLPDLGISTLEDVLVDVRRITDAVDTPLLVDIDTGFGGAFNIARTIK
                     SVAKAGAAAVHIEDQVSAKRCGHRPNKAIVSLEEMADRVKAAVDAKPYDDFVVMARTD
                     ALAVEGLQSAIDRACACVEAGADMIFPEAMTDLTMYKQFVDAVKVPVLANITEFGATP
                     LFTVDELASADVSMVLYPLSPFRAMNQAALNVYQAVKKDGTQKNVLNTMQTRMDLYDH
                     LSYHSFEQKLDALFNQNKS"
     gene            17232..18386
                     /gene="prpC"
                     /locus_tag="PKL42_01635"
     CDS             17232..18386
                     /gene="prpC"
                     /locus_tag="PKL42_01635"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HNU65800.1"
                     /translation="MSDAATTLEPKRKKGVALAGVAAGTTAICTVGHTGNDLHYRGYD
                     IIELAKHTTFEEVAYLLIHGALPNQAELTAYHARLKKLRGIPQALKEVLERIPKTAHP
                     MDVMRTGCSVLGTLEPETADRNIHDAKKLGDRLIACFGSILIYWYQFSHNHKRIDVES
                     DEDSIAAHFLHLLHGKKPSDLHIAAMNTSLVLYAEHEFNASTFTARVIAGTLSDMYSC
                     ITGAIGALRGPKHGGANEAAMEIIERYKTADEAETDIMARMARKEIIIGFGHPVYTIA
                     DPRNESIKAVSRKLSEDNGNMTLFDVSARIEETMWREKKMFPNLDWYSASSYHMMGIP
                     TGMFTPIFVISRTTGWVAHVIEQRIDNKIIRPNAEYTGPDNRPYVPLAQR"
     gene            18404..19864
                     /locus_tag="PKL42_01640"
     CDS             18404..19864
                     /locus_tag="PKL42_01640"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HNU65801.1"
                     /translation="MSSHISNVRPAPDQVIVDIANYVADYQINSDEAFDTARNCLMDT
                     LGCGFEALDYPACTKLLGPVIAGTVVPNGAKVPGTSYQLDPILAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILAVADYISRKNVSEGKAPLTIKDVLTAMIKAHEIQGVLAL
                     ENSFNRVGLDHVILVKIASTAVVTKLLGGDKEDIINAVSNAFVDGQSLRTYRHSPNTG
                     SRKSWAAGDATSRAVRLAWMTLQGEMGYPSALTAKTWGFYDVLFKGNAFKFQRPYGSY
                     VMEQVLFKISFPAEFHAQTAVECAIQLNAQVGSKLQTMEQIAAIDKIVITTHESAIRI
                     IDKTGPLDNPADRDHCIQYMAAIGLLKGNLTAQDYEDAVAADPRIDAIRAKMVCVEKA
                     EYTRDYLDPEKRSIANAIQVFFKDGSTSENIAVEYPIGHRRRRGQGIPELVKKFKVNL
                     ARRFDAKKQDEILALCLDQNKLEETSVNTFVDMLSA"
     gene            complement(19995..20999)
                     /gene="haoB"
                     /locus_tag="PKL42_01645"
     CDS             complement(19995..20999)
                     /gene="haoB"
                     /locus_tag="PKL42_01645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine oxidation protein HaoB"
                     /protein_id="HNU65802.1"
                     /translation="MLKSLKQACLVLFSGVLLIAGGTLLYKQYKLSSMKQDADAPLMV
                     KRTQEKTSDLRGVFPVDENSRYTFQKNGKDAFSLLVANYKNQNAQASNAILFPAGTHD
                     SLTPNTTALRQSIWVETAKLIQTHTPENALILSWWDDGQRINFLSGREGWLNKPSTET
                     FKSNLWKKLRGNLLLAPSAEKSRLNQMARWLTMDSDKALTEIRSTFGSQRPIYFVVNN
                     DLLMRVREMEDYGGTPLLFNTIALQARDDLHGDINQIRRWANEEGDGNYLVQKEGSYY
                     RAWTTPKNSDAEDNTLLARLLPFVDSLKKLPENVDLVYQSRWGGYLSIYKIEFKATSP
                     "
     gene            complement(21004..22665)
                     /locus_tag="PKL42_01650"
     CDS             complement(21004..22665)
                     /locus_tag="PKL42_01650"
                     /inference="COORDINATES: protein motif:HMM:NF024839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HNU65803.1"
                     /translation="MKNLIGWILTTFLASLIGLGLINTAWAVEHDFEKIKKNYYDTHP
                     GKGSHGKFWEPIPIQKYWNPKDFYSPPQTIKGEFTQSDCVGCHQTTTPGAFHAWKSSS
                     HSDLEAIRKLPDSDARAYKKQKITEVENNLRKLNLLKKGEPLKQVGCIDCHGGVGVTK
                     IDHAKALVMPDRVSCGTCHLNEFAEAESEKEQEWPQKQWAKGHPSHAVDWEANVETAT
                     WAAMPEREVAQGCDSCHYQQNKCDGCHTRHTFSAAEARQPEACATCHNGVDHNEFENY
                     MLSKHGTQYLTMGKTKWNFEVPLKDAVGKGGYTAPTCQTCHFEYKGEYSHNLVRKVRW
                     GFNPTPEIANNLSHPWFEDRKKAWVQTCTLCHSESFAKAYLDTADKGTIQGLNVEQSA
                     KKVVNKLYEDGLLVGQKTNRPAPPAPEKDAAGGFFQLFWAKGNNPSRIERLYADMWEH
                     DVIKHYKGIFHVNPGGYTYSEGWSELMRGYAEIMDEDTRLREAADAAKAGKEVKVSDA
                     SQLGSENMLGGGGLLLMIAGTLVGFPALRSSLLSFRSKRRKLSDK"
     gene            23272..24108
                     /locus_tag="PKL42_01655"
     CDS             23272..24108
                     /locus_tag="PKL42_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEPxxWA-CTERM sorting domain-containing protein"
                     /protein_id="HNU65804.1"
                     /translation="MNFKIATIGLLSLFAASAQAAVIGSDAGIKADIVASGNFNNAGD
                     DGTLGLSYNGKEWINWGTHSSWTWLSASNLASPVNTLGGNNPLGASILASGSNFVLNG
                     FFDGLTFIQTATLNNPNQIGFTISLTNNTGKDITDVFWGVGLDPDINIAFGTSNYATI
                     NAITGLGGNAAVSASGSGQTLTLANTTSASAFNIAAYINGGDCCSPVDPAVALAAGQI
                     LGYSTNSDDSISLAYGLGTIGIGKTVTFGYSIAAVPEPETYALMLAGLGLIGFTARRR
                     KN"
     gene            24538..25908
                     /gene="purB"
                     /locus_tag="PKL42_01660"
     CDS             24538..25908
                     /gene="purB"
                     /locus_tag="PKL42_01660"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNU65805.1"
                     /translation="MSLTTLNALSPLDGRYQSKLDALRPYFSEYALIKHRAWVEVEWL
                     KALSAAKDLQEIAPFSAETIKELDTTIANFSEVDAAQVKAIESRTNHDVKALEYWLKE
                     KFDANPEIKKASEFIHFSCTSEDINNLSHGLMLKTARDSVMLPFLNNLIARLSELAQQ
                     LAAQPMLSRTHGQTASPTTMGKELANVVFRLQRQQKQFKNNEILGKINGAVGNFNAHL
                     SAYPTFDWENFAKHFVESLGLAYNPMTIQIEPHDYMAELFDTIARINTILIDLNRDIW
                     GYISVGYFKQKVKAGEIGSSTMPHKVNPIDFENSEGNLGLANAVLRHMAEKLPISRWQ
                     RDLTDSTVLRNMGVAFGYTLLGFDSCLRGLNKLEINAAKLAEDLDNSWEVLAEPIQTV
                     MRRYGIENPYEQLKELTRGKGGINKDSLHVFISGLAIPHDAKVALLALTPATYIGKAT
                     ELAKHI"
     gene            25892..>26126
                     /locus_tag="PKL42_01665"
     CDS             25892..>26126
                     /locus_tag="PKL42_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65806.1"
                     /translation="MRSIFNVAGMKSTLQHFSPTTMNSQIAVIGRLFFQSIFLLAFLL
                     TCPTTQAAELQYQVEIIVTAENANGQVAAIKSLL"
BASE COUNT         7092 a         6301 c         5506 g         7227 t
ORIGIN      
        1 gccaggaatt tcggttcttg cgccagccac accaaaagtt gagtacacac cttcatcacg
       61 caattgcgct tcatccatgc gagttggtgg tgcaatccac aaccgggtag gtatattgtt
      121 tgagccttcc aaaacttttg ccgctgcgcg ataatggccg atattggtca tacaactgcc
      181 aataaatact tcatcaattt tgtcccccac gacggctgac aatggtttga tgtcatccgg
      241 atcgttcgga catgccacga gtggctcttt aatggtattt aaatcaattt caatcacatg
      301 cgcgtattgg gcatcagtgt ctggtgttaa taactcaggt ttagccaacc aagcctgcat
      361 attctcgata cgacgctgca aagtccgcgc atcttggtag ccgttatcta tcatattttg
      421 tagcaacaca atattagagt ttaaaaactc aatgactggt gcattgttta gcaacaccgt
      481 acaaccattg gcggaacgct ctgcggaggc atcggcaaac tcaaacgctt gctcaacttt
      541 taaatccggc agaccttcaa tttctaaaat acgcccgtta aatacattct ttttgcctgt
      601 tttacctacg gtgagcgacc cttcttgaag ggccgcgtaa ggaatcgcat tcaccaaatc
      661 acgcaacgta attcctggct gcatggtacc tttaaagcgc actaatactg actctggcat
      721 atctaacggc atggcgcctg tagctgcagc gaaagctacc aagcctgaac ccgcggggaa
      781 tgaaatacca attggaaaac gcgtatgtga gtcgccgccc gtacccacag tgtcgggtaa
      841 aatcatacgg tttagccatg agtgaatcac gccatcaccg gggcgtaatg aaacgccacc
      901 tcggcttgat atgaattcag gtaattcgtg ttgcaactta atatcaaccg gttttgggta
      961 agcggcggta tgacagaagc tttgcatcac caaatcgcta ctaaatccta aacacgccaa
     1021 ctcttttagt tcatcacgcg tcatgccgcc agtggtgtct tgtgaaccta ctgtggtagc
     1081 tatgggctcg caataagtat ttggccgcac gcccacgcct tctggcaagc cgcaagcgcg
     1141 gccaaccatt ttttgcgcaa gggtgtagcc tttgcttgag gtaacctcag gtgttgcttt
     1201 aataaaaata tccgtatcat ttaagcccaa tgccttacgt gcattcgctg ttaatccgcg
     1261 accaataatc agcggtatac ggccacccgc gcgcacttca tctggcatcg tgactgggtt
     1321 tagggtgaac gtcgcgataa tttcacctgc ttcgttgagt actttgcctg catagggttg
     1381 aatggtaatt acatcccccg ttgccatttt tgaaacatcg cattgaattg gcagtgcgcc
     1441 agaatcttcg gcagtattaa agaaaatagg cgcaattttg ccgcctaaaa tcacaccacc
     1501 tgtgcgcttg tttggaatat gcggaatatc attacccata aaccattgca ctgagttaat
     1561 agcagatttg cgtgatgagc ctgtaccgac cacatcgccg acataggcga gtggcaaacc
     1621 tttttgcttc aaagcatcaa tgcgcttcaa gccatcaggc attttattaa tcagcatggc
     1681 ttttgcatgc aatggaatat caggccgact ccacgcatct tgtgccgggc ttaaatcatc
     1741 ggtgttggtt tcgccgtcta ctttaaacac aaccattttt aattcttgtg cgagcgctgg
     1801 cgcattagtg aaccactcag cattggccca agattcaatc agctttttag cgtgcgcgtt
     1861 accggccttc atttttacat caacatcatg aaacgcatca aacatgagaa tagtgctact
     1921 taacgctttt acggcatgct ccgccaaggt aggattagaa tcagataagt ctagcagtgc
     1981 aaccaacgct tgtacgttgt agccacccaa cattgtgccg agtaacgcta ccgccttttc
     2041 tgctgaaatg agtggtgaac tcgccttacc attggcaata tctgccaaaa atgcggcttt
     2101 aacataagcc gcttgatcaa caccggctgg cgtacgatta gcaattaaat caagcaggat
     2161 ttcaccctca ccggctggcg ggttttttaa taaagcaacg acaagcgcta cttgctctgc
     2221 gtttaacggt agtggcggaa gttgttgtgt ggcacgctct tgcacgtgcg ctgtataggc
     2281 ttttagcatg gctgattcta tttaggttag tttaaaaaat tagcattatt ataaaccagc
     2341 ccaacagcca tgaggttcga gttcaaactc acttcatgaa aatcaggcta attatttaca
     2401 taaaaatctt tggcaaatgg cgtattgcaa ctggtgccgc gcatggaggc ctcaaattta
     2461 tccagcactg cgctttggta acctttgcca ttaatcagtt ctaagtaggc gcgcacttct
     2521 gaggcgtctg caaagccgtt tttatcttta tccatcaggg caatattatc ttcaaccgtc
     2581 gggccagtat atttgccatt ggcatctgtt aaataatcca aaacacttgc cgcatgcgaa
     2641 agtgttgcca tggccaataa agtggccgca aatatagtct ttataaaacg acgttgtgtg
     2701 tgcatgattc actccttgtg cgattatgac tcatgttaca cacaaacatt catacactat
     2761 gtgactttac gcacaattag tgtttaagca gtatatttgc ccaaagtgct gggccagttt
     2821 ggtgcactga ctctccgcgc gatgtcaccg caactgtcac tggcatatcc acaatatcaa
     2881 actcataaat cgcttccatg cctaagtctt caaaggctaa cacacgcgat tttttaatgg
     2941 ccttactgac caaatatgct gccccaccca ccgcagttaa atatacagcc tgatgctttt
     3001 taatcgcttc tattgcttct tcgccccgct cggctttgcc caccatgcca atcaaacccg
     3061 tttgcgagag catcaactca gtataactat ccatccgcgt ggctgtggtg gggccggccg
     3121 ggcccaccgc ttcatcgcgt acggcatcca ctgggcctac ataataaata aagcgatttt
     3181 gtaaactcac tggcaacggc tcaccttttg ccatcatatc ggccatccgt ttatgtgcgg
     3241 catcgcggcc tgtgagcaat ttgccgctga gtaataatgt ttcacccgct tgccaagtgg
     3301 cggtttcagt ctttgtaatg gtgtctaaat tcacccgttt agaagccgca cttggctgcc
     3361 aatgcaccgc tgggtacatg cttaaatcgg gtggcgtgag ttcagcaaca ccactgccat
     3421 ccaacacaaa atgtacatgc cgcgtggccg cgcaatttgg aataatggcc accggcaaac
     3481 tggccgcatg ggttgggtaa tctaaaattt tcacatccaa caccgtagtc agaccaccta
     3541 agccctgcgc accaatacct aaattattca ctttttcaaa aatttctaag cgcaattctt
     3601 ctagccggtt ggtggcgcct ttggccttca gttcatgcat gtcaattggc gccatgagtg
     3661 attcttttgc caacagcacg gctttttcgg cactgccgcc aatgccaatc cccaacatgc
     3721 cgggtggaca ccaacctgca cccatttggg gcacgacttc taaaatccaa tccacaatgc
     3781 tctcattggg gttgagcata gcaagttttg ctttattctc tgagccaccg ccttttgctg
     3841 caatctgcac ctctacttga tcacccgcca ccaatgaaac atgcactacc gccggcgtgt
     3901 tatctttagt gttggcgcgc ttgccagcgg ggtcgctcac aatagaagcg cgtaatttat
     3961 tttctggtag caagtaagct tgccgcactc cttcgttcac catttgctca atgtctaata
     4021 ttgcatccca ctgcacacac atccctactt taacaaaagc aacaacaatg ccagtatctt
     4081 ggcaaagcgg gcgtttgcct tcggcacaca tgcgcgagtt cgttaaaatt tgcgcaattg
     4141 catctttcgc ggcgggggat tgctctgcct cgtatgcgcg acccagcgcc tgaatataat
     4201 ctgctgggtg ataatacgaa atatattgca gggcatcttt aatgctttga atgaagtcac
     4261 tttgtttgat tgtggtcatt ttttgaattt actacactcc aattttcgaa gcgttttacc
     4321 tttttaaatg tgcggttatt attgcagttt tcgtgtttaa atcacgcgca aaattcctcg
     4381 agaaaaattt aatgccctgc gagcctttat ttataaggct cacagccctg ccaaaaaggt
     4441 tgcatctaaa tttaagctaa aaaatgcaaa ttttcagcca atattaacta acgcgaactc
     4501 ttagctactg caatttttgg gcttatatct ttacgcaaaa ttttaccatt tttagtacgt
     4561 ggaaaatctt gggccaagta caccgtttta ggcgttttgt atgcggccaa atgctgtttg
     4621 ccatagccta acaattcgtc tgggctggtg cttgcctctg gttttaaaat cacgtaaatc
     4681 actaccagta atttatcttt ctcaatttct tcgcccactg cggcacagtc ggctacatct
     4741 gggtggcctt tatagacgcg ctcaatttca taaggtgaca cgcgatagcc aaaactttta
     4801 ataatgtcat ctttacggcc taaaaaccaa atgtagccat ctgcatcgta ttttgcataa
     4861 tctccagtga aaaagtagcc atcatgctta tactttgcag tttcatcatc taaattccaa
     4921 tagcataaaa agagccctgg gtcgttatct ggcacacaaa tcataccttc ctcacccgtt
     4981 tcaactgctt ctaaagtttc aggattgagc aattgaatat catggccagg ctgtggaaag
     5041 cctgctgaac cggcacgaat gggacgaaat ttgctttgtg ataaataata tgaaaactca
     5101 ctcatgccaa cggcttcata aatttctaag ccaaaacgct cgcgccattg gctcaatacc
     5161 tcatcggaca aatgctcacc cgcactcatg taatggcgca aactgggcaa ctgcgcgcca
     5221 gtagtggtgg ttttttgcag aatttgtcga taaatcgttg gcacaccaat aaaaatggtg
     5281 gcggcatgtt tttgaattaa atctagccat ttttgcgcat catttttgcc ttcatgcaca
     5341 atcaccgttt tgcctaggta aagcgggtcc attaggccag tgcctagcac gtaagtccaa
     5401 ttaaacttgc ctgaatgcat aatgcggtct tgcttttggc ttccatttgc atctgcggca
     5461 tcactgtaat taaaccaata ctgcgcggca ggctggcgcc ctagcaaagc gcgatgcgca
     5521 tgtagcaccc cttttgggta acctgttgtg cctgaggtgt aaaccaaata ggcagggtca
     5581 ttcgcttgtg ttttgtaggg cgcttcgcac tcttgcgttt catgcaatga agccgctaaa
     5641 ttcaacacat taaaagtgct gtgcggtttg gtttctatcg cttgtgacaa caacacatgt
     5701 tttaaagtcg gtgattgacc caaggcattt tcaatttcac ccgccatcac ttcccacgcg
     5761 gcggcgtctg tcaccaaaac agttgcgcca gaatctttgg ctaaatatgc cacttcttct
     5821 gcggtgagca aggttgaagt gggtacggaa atcgcgccca ttttcatcgc gcctaaaaag
     5881 gctattggat aatctaagct attgggtaag cgaatcaaca cgcggtcacc cacttgcaca
     5941 ttgagattac gtagcacttg cgcaaattgg tcggttttac gcgacaattc attaaacgtg
     6001 atttgcgaag tgcctaatac gtcgtcttca acaatcatgg caatattact ggcctgcggc
     6061 gtgcctaaat ggcggtctgt gcaggccacg cctatgttaa acgctttggg aataatccat
     6121 tgccactcgg gaaattttgt aaacattatc atgactcaat cctttaaaaa attcagtttt
     6181 tcgagatgaa gcgctaggcg cactgcgcac agaaagcgga atgtacacaa agtacatgag
     6241 catttcgagc acagcgtaac gccgcgattc gctcgaaaaa tggatttttc ataaaagcac
     6301 tccatatggc cagttttgac gtaccacttg cgcgggtaat aattgcaaca catgtactgc
     6361 aaaatccatg tcacttgttt ttagcgctcg taaagcgtgt gcccgtaata aggtttgcag
     6421 cgctttacca aacatgggcg gatctaacat ctcaccctca aatttaatcg cgcctccatg
     6481 caaggcatcc gcctctatcg cagcttttaa aatggctact ttctgcgcca catcggtggg
     6541 tgatggcgtg taagcggttt tacataaatg aatatgggct gggtgtatca cttgcttgcc
     6601 cgttaacccc atcgccgctt cttggcaagc atgcttgtac accacgcgag attcgttatc
     6661 gccatgtaaa tcgagccagc ggcgcacatc atgcggctct aaaaattttt ctggcatggc
     6721 gctggcacca atgagtgttt ccacgccgcc aatcacgcct ttacccgcaa tacgcgcttc
     6781 aaacatgagt tgattcatgt aaaactgcaa ttcttcaatc caattttctg gcgtaatgtg
     6841 gatgcccatc gcttttgaaa agtcatgaat accaaacacc acatgtttca ctgtgttgta
     6901 ttgcatgagt tcaggcgcaa ttttgagcga ttttggatgc tcaataatcg gctgaatggt
     6961 gatgttgggg ttgacgctga gtaaaaagct cgataaatcg cgcacttcag ccgcaccgta
     7021 cgcttcacct gctttggcta gcatcaccac atcaatatat tcacccattt tgcataccaa
     7081 cttcatgtcg agctcgtatt catcggtgcg gaaagaatta cagcgaattg ccaccgtgac
     7141 gcgcggattc atttttttga gcatcggcaa ctcttgccgc aacaaatcgc gactcattac
     7201 tttttgtcga cagccatctt ctaagtcaaa aataagcgta tgcgcactgg taaaatgcgc
     7261 gtgctttttc atccgcgctg aagcttgcgc catgtcctca taaataccca aactcggggc
     7321 atatttaacg gggggataat acagctgaat gcccggccaa taatcgccca acgcacttgc
     7381 atcaagtgat aacgcctgtt cgcctatgtg catcatcatc ttaagcaccc tcctgatcgt
     7441 taaatcgtac cgcttttatt ttgtgaatga ctgccgttaa atgcgcatca ttttctggct
     7501 gttttgagcc gttaagtaaa tcccaaaaat cgttatcagg cagctccagc atggcatcaa
     7561 acacacgcaa ctcacctaaa tttagctgat tgaactctgc cttcacaaag cgctgcaaga
     7621 caatatccag ctctaacatg ccgcgcctgc agcgccatgc aagccttttt tgttcggcat
     7681 cgagtatatt ttctgcgttc atcatgattt aacccacctt aataccaaag atttttttag
     7741 ctggtttttt atcttttgcc cgtgcaattt ttaaatcttt aatattggca atggccgcat
     7801 ttgggttgag ttttttcggg cacacttcaa cacaattcat aatgttatgg cagcggtata
     7861 aacggtatgg gtcttctagg ttttcaagcc gttcttctgt tgcctgatcg cgtgaatcca
     7921 taataaaacg attggcttgc attaagcctg atgggccgac aaacttatcc gggttccacc
     7981 aaaaactggg gcatgaagtg gtgcatgcac cgcataaaat acattcatac atgccatcga
     8041 gtttggcgcg atcttcaggg ctttgcaggc gctcagtttc gggtaatggg tcatgattga
     8101 ttaaataggg tttgaccgag ttgtaatgct caaaaaactg cgtcatatca acaattaaat
     8161 cgcgtatcac cggcaagcct ggcatggggc gcagcacaat tggctcttct aaatccgcca
     8221 atttggtgat gcaagccaag ccatttttac cgttgatatt catcgcatcg ctaccgcaaa
     8281 caccttcacg gcatgattta cgcaagctta aagtgtcatc cagttcttta atgcgtagca
     8341 atgcatccag caacatgtgg tcttcttctt gcaatgccac atcaaactct tgcatgtatg
     8401 gctttgcatc tacatcgggg ttatatctgt aaatactaaa tttcatatta ataaaccctt
     8461 ttcttaggct caaaactctc taccgtgagc ggtgttaatc gcacaggctt atacgtgatt
     8521 gcattgtttt gtttaaaaaa caatgaatgc tttaaccagt gcgtatcatc gcgctcagca
     8581 aaatcttcac gcgcatgggc gccacggctt tcttggcggg catgtgcggt acgcatggtg
     8641 gccacagcca cttcaattaa attatccaat tccaacgcct caatacgggc ggtgttaaac
     8701 acttggcttt tatctttaat ggtgatgtgc tgcgcgcgtt ttgccacagc atctatcgca
     8761 acaacgcctt cagataacaa ctctggaaag cgaaatacac cacagtgcgt ttgcatggtt
     8821 ttgcgcatgg cagcccctac ttcactcaca ttttcgccct gtgcattttc gccctgtgta
     8881 ttttctagcc gcgcaatgcg tgccaaggtg gcatctgcca cattggcagg caagggttta
     8941 tgggctttgt tttgtgcgat ttcttgcacc attttgtcgc cagcggcttt gccaaataca
     9001 actaagtcaa gcaatgaatt tgaacccaaa cggtttgcgc cgtgcactga aacacaagca
     9061 cattcgccta ccgcatacaa tccactcaca gttttttcac ctgttttttc atcaaacagc
     9121 accacttgtc catctaaatt ggtagggatg ccgcccatca tgtaatgcgc cgttggcacc
     9181 acaggaatgg ggtctttgat tgggtccaca tttgcaaatg ttttagcaat ttcgcggatg
     9241 ccaggcaaac gtttactgat gagttcttca cctaaatggt ctagttttaa ataaaccgca
     9301 tctttatttt tgcctacacc gcgtccttct ttaatttcgg tggctgaagc acgtgaaatc
     9361 acatcacggc tggctaaatc tttggcgtgc ggcgcataac gctccataaa gcgctcgccc
     9421 tcactattga ctaaataacc gccctcacca cgaacaccct cggtaatcaa cacacctgca
     9481 tgcaatacgc ctgtggggtg aaactgccaa aattccatat cttgcagagg caagccagcg
     9541 cgtgccgcca tacctaagcc atcgcccgtg ttaataaaag cattggtgct ggcctgaaaa
     9601 attcgcccag caccgcccgt agccagcagt gtggttttgg cgtgtaaaat atgaatctcg
     9661 cctgtttcaa tttccagcgc aatcacaccc agcacatcgc catcggcatc tcgaattaaa
     9721 tccagcgcaa accattctac aaaaaacttg gtgttggctt gaatattgcc ttgatacagc
     9781 gtgtgtaaca tggcgtggcc cgtacggtct gccactgcac aggtgcgcga aatgggcgtt
     9841 tcgccaaaat tttgcgtcat gccaccaaac gggcgctgaa aaattttgcc gctggctagc
     9901 ctatcaaacg gcataccaaa atgctcgagc tcgataatgg cttttgcagc gtttttgcac
     9961 ataaactcaa tggcatcttg gtcgcccaaa aagtcactgc ctttaatggt gtcgtacatg
    10021 tgccacagcc atttatcgtc gctcacattg cctaaggccg ctgcaatgcc accctgcgct
    10081 gctacggtgt gcgagcgggt aggaaacact ttcgacagca ccgccacctt cacgcctgct
    10141 tccgctagtt gtaacgaggc gcgtaagccc gccccgccgc cgccgataat cacggcatca
    10201 aattgatgta tctgtaagct catagacacc tataaattcc acaaaataaa acacaaccaa
    10261 acaaaataaa tgaccagtaa aacatctaca atgatttgca aatagccacg caaaccctga
    10321 ttaaatacgt agtctcgcaa cacatcgcgc acccctagcc atgcgtgcat acaaagtgaa
    10381 gcaaaaaaca aaatcgtggc taacctaaac caaacgggtg atactaaggc atgccaatcc
    10441 tcaaaccgcg ctggctgaac aatgactaaa agcaatatca ccaacaccaa gtaagccgcc
    10501 atcaccaacg ccgttaagcg ttgcgtcagc caaaagcgca tgcctggata tttatcggtt
    10561 aacagttcaa ttagcatcaa ttcaccaaat acttatgcca aaaatcacca cagaaacgcc
    10621 caccaaccac aacaccacac ggctggaaaa acgcgctttt tggagtgaag tcatccaatg
    10681 tacgtcttgc aataaatgac ggataccagc aaaaaagtga tgaaaaaacg cccacgacaa
    10741 acctatgagc agcaatttaa cccaccaagc gccgaatata tttgccactt ccgcgtaact
    10801 ttctggcgac ttcactgaag cctgaagggc caacagcagc aagggcaaca ccaaaaacaa
    10861 ggccacacca gaagcacgat gcaatatcga caccaccgca ttaatcggta gccgaatcgt
    10921 gaacagattc agattttttg gcctattttt ttgttggttt tttttcatca cacttaactt
    10981 agcgaatatt attttgccgc cttggccacc gcacccgcca cccgtgctaa caggcgcggg
    11041 tcaaacggtt tagggataat gtattcctta ccaaatttta attctgttaa attgtaagct
    11101 tgcagcactt cagctggcac tggctcacgt gccaattcac ttaaggcgtg ggcggcggca
    11161 atcttcattt cttcggtaat ttggcgggct tttgcatcga gcgccccgcg aaacaaatac
    11221 ggaaagcaca aggcattatt cacttgattt ggaaaatcac tgcggcctgt cgccattaaa
    11281 acatcatcgc gaatcgcgtg cgccacactg ggcaacactt cagggtcagg gtttgctagc
    11341 gcaaaaataa tcggattttt ggccatactt tttattaagc ttgcatccag cgcatttttg
    11401 gcagatacgc caataaatac atcggcatct ggcatcacat ctgccaacac tttcgcatca
    11461 ctcggtacaa cagctaactc acgattgtta tcatgcaagt ctgctctatc cgtgtccagt
    11521 acaccgcttc tatcggtcat ggtgatattt gttgcgccca tcagtttaag taattttgca
    11581 catgaacaac ccgccgcgcc cgcacctaaa aatacaattt ttgccttaga gagcgttttg
    11641 ccctgaatac ttagcgcatt gagcaaggca gcggccacaa ttaccgcagt gccatgctgg
    11701 tcatcatgaa aaacgggaat atccaaacgt tttttgagtt cgttttcgat gtaaaagcag
    11761 tgcggcgcgg caatatcttc taaattgata ccgccaaacg taggcgcgat attcaccacg
    11821 gtttcaataa aatcatcagg cgtttttgcg ttcacttcaa tatcaaacac atcaatgccg
    11881 gcaaaccgtt taaacagcac ggctttgcct tccatcacag gtttactggc taacgcgccc
    11941 atattgccta agcccagcac cgctgtaccg tctgtaatca ccgccactaa attgcctttg
    12001 ttggtgtatt tataggcatt ggctgggtct tcatgaatct cacgcacagg cgctgccacg
    12061 ccaggtgtat aggccaaaga taaatcatct tgtgtggcgc agggcttaga ggactctaca
    12121 ctgagttttc ctggtattgg aaactgatga taatccaaag cgcgtttttt taagtcgtcc
    12181 atttgttgcc tttcagtgca gttatttttc ttttattgat gaccacattg cgaccatcaa
    12241 ccaaccaaat taaagcgcaa aattcaatgc aaaaaactcg gtttttatag ctaatttaac
    12301 taacaacctt tttcgatgct ctggaagcct tataaataaa ggctctcaga gcattatttt
    12361 tttctcgtag gttttattgt gtttaaacgc atttaattag ttgttctaat tgcggctaaa
    12421 tttactctaa ctcactcatg taatgatggt catccgttaa gcaaagtcca agccgccatt
    12481 ccatcggttt atcgccataa gtaaatgaaa cacgctcaat acttaataaa gggtaacctt
    12541 ctttcaaccc taatgctttt gccacatctt gactggcggc cactgctttt aggcgctctt
    12601 ccgcacgtat catgcgcacg ccaaactgtg actcatacat gctatacatt gacccgttac
    12661 tttcttccac cttggcacta tttaagcctt taaaaggtgc ggctggcaca ataatgtggt
    12721 cgtatattaa gggcctgcct gaaaaagtca gcagccgctt cacttcaatc gtggcagcac
    12781 ctgctttaat atcaagcact tgcgcaatgt gcgcatcagc ttttgatttt tcacaagaga
    12841 taaactcatt attcaataat tctttttggc cgctgactgc cgtcagcctc aaaaaccgca
    12901 acttcatacc ttcggcttca tgagtggcaa caaaagtacc cttgccctga cgccgaatca
    12961 aaatattttc tgccgccaaa ctatcaatgg cttttcttac cgtgccttgg ctgaccttat
    13021 atcgcgctgc cagttcaacc tcgcttggaa tcatctcgct tgggcgccat tcacctgcaa
    13081 tcaggctttg cgtaatcagc accttgattt gatcatatag cgggcgataa ctgggtgaat
    13141 ttgattgctt gtccattttt ctaaccattt ctaaagtata tttcgactca atcggcatgt
    13201 gatatttcag cgtttcgata tttatagcat gacccattta atatgtctag cattttatat
    13261 cttatataag acatatgata tatgttgaca ttttcattcc accctctata taatcgttct
    13321 aactttaatc aacccaacta aaaataatgg aatattgcct acgccctcga cgatccatgc
    13381 tgtatgtgcc gggttgcaac cagcattatt tggatcgcgc tcgtacgctg gctgccgatt
    13441 cggtcatctt ggatttaggt gatccaatac ttattgatgc aaaattgcaa tccagagaca
    13501 acgttgttgc tgctgtgaaa caaggcggct acggctcgcg cgaggtagtt gtacgcgtga
    13561 ataatctgga ttcaatttgg gggcacgatg acatcaaggc cgtggccaat attggtgccg
    13621 atgccatttt gtttcctaat atcgagtctg ccgagcacgt gcatactgcg cttgagttgc
    13681 ttgatgcggc cggtggcggc catatgccga ttatggtgat gattgaaagc ccgctggccg
    13741 tgctgaatgc aaaagaaatc gccagcgcgt cagaccgtat cacctgtatt gtgatggcta
    13801 cttcggattt aatttctcag ctgcatgcgc gcgttacgca cgagcgctcg gcgatattga
    13861 cttcactttc attagttgta ttagcagcgc gcgcgtatgg tcgctgcgtc attgacggta
    13921 tcaccagtga ttttaaaaac atgcattctt ttgagtatgc ttgccgatta gggcgagaca
    13981 tggggctgga cggaaaatcc ctcgttcatc cagcacaaat cgcttactgt aatgatgcct
    14041 acacgccaaa agctagcgaa gttgccaacg caagactgat tatcaaagcg ttacaagaag
    14101 ccaacgaagg cggcttaggc accgttgttg ttaacgacaa attagttgaa cagcaccatg
    14161 taaaagctgc gcaacggtta ctaaaactga gcgacatgat taccgaactt gaagaagact
    14221 ttatataaat gacactttct accaaaatca atgcaggccg tttttttgaa gactttgtgc
    14281 tgggcgaagt aattttacat gccacgccac gcaccattac cgcaggggat gcggcgcttt
    14341 atatcgcgct aactgggtca cgcaacccat tgcattgcag cgagccattt gcgcaatcgt
    14401 taggctacaa atccacaccg attgataatt tattggcttt tcatatcgct tttggcaaaa
    14461 ccgtgcccga tatttccgtg aacgcggttg ccaatttagg ttatgccgag gctaaattta
    14521 ttcaaccagt gtttgtaggt gatacgctga ctacaagtag cgaagtcatc ggcctgaaac
    14581 aaaactccaa cggcaaaaat ggcgtggttt gggtgcgctc caccacggtt aatcagcaca
    14641 aagtggctgt tatgagctgg gtgcgctggg ttatgattca taaaagtgag catcatcaaa
    14701 ccaatctttc tgcacctgcg ccagagacgg tgattccaga attggcgcca tttgtcagcg
    14761 ttgaacactt gcagttatcg ccttatatgt cggccaaata ttaccaaacc aacgccacag
    14821 gcggcgacta tttgtgggaa gactatcaaa ttggtgagcg catcaatcat gccgccggca
    14881 tgaccatcaa tgagagcgac cacagcttgg cgacaaggct ttatcaaaac aatgccaaat
    14941 tacactttga cgcgcacttg atgaaaaact cagccattgg ccagcgtttg gtttatggcg
    15001 gcgtggtaat ttctgcttgt cgcgcgttga gcttcgatgg tttagaaaac gccctcagca
    15061 ttttagccat caacggtggt acgcatgcaa atccatgctt tgcgggcgac accatttaca
    15121 catttactga agtgttagac gcatggccac tgccaaaacg taccgatatt ggcgcattgc
    15181 gcttgcgtat ggtgggcatt aaaaatatgg ccgcccatga aataggcgaa attaaaacag
    15241 aaaatggcta tcacccaaat gtggtgctag accttgatta caccgtgcat atgccacgca
    15301 aacattaata acaaagtatt acagctaaag gaaagcacca tggcaaccct tgtccaccct
    15361 tctgaagcac tttttggcgg cgaaaaacct ttcccgctca tccctgcctg cgagcatttt
    15421 gcagggagcg aaaaacttat tttaaaagcg ctttcgctgc aagatactat cggaccagta
    15481 tttgatatta cctgtgactg cgaagatggc gcagcttcag gcttagaacg cgaacacgct
    15541 gagatgattg tgcgcgtact gaattcagac gccaatcaac acaaaatggc tggcgcgcgt
    15601 attcatgatt acacacaccc tgcctggaag ttagatattg atattttggt ggcaggtgct
    15661 gggcaagtat taagctacat cactattcca aaatgcacgg atatcgcgca ggctaaagaa
    15721 atgattgcct acattcaaaa agtggcaacc tttagcggca tcacccgcgt gattccagta
    15781 cacattttaa ttgaaacgca cggcgccctc tatcaagtgc atgaaatcgc caagttacct
    15841 tggttgcaag tgcttgattt cggtttaatg gattttgtca gcgcgcatca tggggccatt
    15901 cctgcccttg ccatgcgtag ccctggccaa tttgaacatc atttgcttgc ccgtgccaaa
    15961 accgaagtag ttgccgcggc cctcgccaat ggcgttgtac cagcgcacaa cgtgacgctt
    16021 gatttaaaaa acgttgagac caccctaagc gatgccacac gcgcacgtaa tgaatttggc
    16081 tttttacgca tgtggagcat ttaccccacg caaattcagg ccattgtgga tgccatgaaa
    16141 cctaattacg atgaagtgat agatgctgcc aatattttaa ttgccgcaca agatatggca
    16201 tgggggccaa ttcaatatgc tggtgaactt catgacagag caacctaccg ttatttctgg
    16261 gaagtgttgc aaaaggccaa actcaccaat gtgaccattc cagacgaagc gcagaaaagg
    16321 tttttttaat gtcaaattta atcacggcag gcgcacgttt tcgtgcagct attgcacaag
    16381 aaaagccgct acaaattatc ggtgcaatca acgcttacca cgccatgctt gccacccaaa
    16441 ctggcttcaa agccatttat ttatcgggcg gcggtgtggc ggctggctca ctcggcttac
    16501 ctgatttagg tatttccacg cttgaagatg tgctcgtgga cgtgcgacgc atcaccgatg
    16561 ccgtcgatac gcctttatta gtggatatag acacgggttt cggtggcgct ttcaatattg
    16621 cccgcaccat taaaagcgtg gcaaaagcag gtgctgcagc cgtacatatt gaagaccaag
    16681 tatccgccaa acgctgtggt catcgcccca ataaagcgat tgtcagctta gaagaaatgg
    16741 ccgaccgtgt aaaagcggct gtggatgcaa aaccatacga tgattttgtg gttatggcgc
    16801 gcaccgatgc gctggctgta gaaggtttgc aatctgccat tgaccgcgct tgcgcctgtg
    16861 ttgaagcagg ggctgatatg atttttcctg aagcgatgac tgatttaacc atgtataaac
    16921 agtttgtgga tgcagtaaaa gtaccagtat tagccaacat taccgaattt ggcgcaacac
    16981 cgctttttac tgtggatgag ctagccagcg ccgacgtgag catggtgctg tatccgcttt
    17041 caccttttcg cgccatgaat caggccgcgc tgaatgttta tcaggcggta aaaaaagacg
    17101 gcacacaaaa aaatgtgctc aacaccatgc aaacccgcat ggatttgtac gaccacttga
    17161 gttatcactc ttttgaacaa aagctcgatg ccttatttaa tcaaaacaaa tcataactga
    17221 gaggaaacag catgtctgat gccgcaacca ctttagaacc taaaaggaaa aaaggcgtgg
    17281 ctttagcagg tgtcgccgcc ggcacaacag ccatttgcac cgttggccac accggtaacg
    17341 acctgcatta tcgtggctat gacattattg aactcgccaa acacaccacg tttgaagaag
    17401 ttgcttattt gctcattcat ggcgcattac cgaaccaagc ggaacttact gcctaccatg
    17461 cgcgtttaaa aaaactgcgc ggcattcctc aggccttaaa agaagtgctg gagcgtattc
    17521 caaaaacagc acacccaatg gacgtgatgc gcacaggttg ctctgtgctc ggcacactcg
    17581 aacctgaaac ggcagaccgc aatattcatg atgccaaaaa gctgggagac cgcttaattg
    17641 cctgctttgg ttccattttg atttattggt atcagtttag ccacaatcac aaacgcattg
    17701 atgtggaaag tgatgaagat tcaatcgccg cgcacttttt gcatttattg cacggcaaaa
    17761 aaccttctga cttacacatt gccgcgatga acacttcgct cgtcttgtac gcagagcatg
    17821 aatttaatgc ttctacgttt accgcacgcg taattgctgg cactttatca gatatgtatt
    17881 cctgtatcac gggtgccatt ggtgcattgc gtggaccaaa acatggcggt gcaaatgagg
    17941 cggcaatgga aatcattgag cgttataaaa cagcagatga agccgaaact gacatcatgg
    18001 cacgtatggc gcgcaaagaa attatcattg gttttggcca tcctgtgtac actattgccg
    18061 accctagaaa cgaatccatc aaagccgtta gccgcaagtt aagtgaagat aacggaaata
    18121 tgacgctatt tgatgtttcc gcacgcattg aagaaaccat gtggcgtgaa aagaaaatgt
    18181 tcccaaatct cgattggtac agcgcctcca gctaccacat gatgggcatc ccgacaggca
    18241 tgttcacacc gatttttgtc atttcacgta ccacaggctg ggttgcgcac gtcattgaac
    18301 aacgcattga caacaaaatc attcgcccaa atgccgaata tacggggcca gataaccgtc
    18361 cctacgttcc actcgctcaa cgttaatttt aagaaagctt ttcatgtcta gtcatatttc
    18421 taatgtacgc cccgcgccag accaagtgat tgtcgatatt gccaactatg tggccgatta
    18481 tcaaatcaac tcggatgaag cctttgatac cgctcgcaat tgcttgatgg atacgctggg
    18541 ctgtggtttt gaagccctag attacccagc ctgcaccaag ttgctaggcc cagtaattgc
    18601 aggaactgtg gtacccaatg gcgcaaaagt accaggaact tcataccaat tagatcccat
    18661 tttggccgca tttaatatag gcgcgatgat acgctggcta gactttaacg atacctggct
    18721 tgccgccgaa tggggtcatc catcagataa tttaggcggc attttagcgg tggctgatta
    18781 tatttcacgc aaaaatgtga gtgaaggtaa agccccgtta accattaaag acgtgctcac
    18841 cgccatgatt aaagcgcatg aaatacaagg cgtattagcg ctagaaaata gctttaaccg
    18901 cgtggggtta gaccatgtaa ttttagtcaa aatcgcctca acggccgtgg tgactaagct
    18961 acttggtggt gataaagaag acattatcaa cgccgtttct aacgcgtttg tggacggcca
    19021 aagcttgcgc acttaccgcc attcacctaa tactggctca cgtaaatcat gggcagcggg
    19081 cgatgccact tcacgcgcag ttcgcctagc ctggatgact ttgcaaggtg aaatgggcta
    19141 tccatctgca ctcacagcaa aaacctgggg cttctacgat gtgttgttta aaggcaatgc
    19201 gtttaaattt caacgcccat acggtagcta tgtgatggaa caagtcttgt ttaaaatctc
    19261 atttcctgct gagtttcacg cacaaaccgc ggtggaatgc gcaattcagc tcaatgcgca
    19321 agtcggcagc aaattgcaga cgatggaaca aatcgcggcg attgataaaa ttgtgattac
    19381 aacacatgaa tctgcgattc gcattatcga taagactggc ccactagata acccagcaga
    19441 ccgcgaccat tgcattcagt acatggctgc cattggatta ctaaaaggca atttaactgc
    19501 gcaagattac gaagatgctg tagcggctga ccctcgtata gatgcaattc gcgcaaaaat
    19561 ggtttgtgta gaaaaagctg aatacacgcg tgattaccta gacccagaaa aacgctcgat
    19621 tgctaacgcg attcaagtgt tctttaaaga cggttcaact tcagaaaaca ttgcagtgga
    19681 gtatccaatt ggccatcgtc gtcgtcgcgg tcaaggcatc ccagaactcg tgaaaaaatt
    19741 taaagtgaat ttagcgcgcc gctttgatgc taaaaaacag gatgagattt tggctttgtg
    19801 tttagatcaa aataaattag aagaaacttc tgtgaatacg tttgtagata tgttatctgc
    19861 ttaaaaattt caaaaataag ctgcgagcaa aaaatactgc cctgcaagcc tttatttata
    19921 aggcttgcag ggcagtacta taggagaccg caattctaaa gaaaacttat attgttaacg
    19981 gcacgctttt acgctcacgg gctggttgct ttaaactcta ttttatagat tgataaataa
    20041 ccaccccaac gactctgata aaccaaatca acattttctg ggagtttttt aagagaatct
    20101 acaaagggta gcaatctggc tagaagcgta ttgtcttctg catcagagtt ttttggcgtt
    20161 gtccaagcgc ggtagtaact gccttccttt tgcactagat aattaccatc cccttcctca
    20221 ttcgcccaac gcctgatttg attaatatct ccgtgtaaat catctcgagc ctgcaatgct
    20281 atagtattaa acaaaagcgg cgtaccgccg taatcttcca tttcccttac acgcatgagt
    20341 aaatcattat tcacaacaaa ataaataggg cgctgtgagc caaaagtgct tctaatttcg
    20401 gtcaatgctt tatcgctatc catggtgagc cagcgtgcca tttgatttaa acgactcttc
    20461 tcagcacttg gggccaacaa caaatttccc cgtagctttt tccacagatt acttttaaaa
    20521 gtttcagtag agggcttgtt taaccaccct tcacgaccac ttaaaaaatt aattcgctga
    20581 ccatcatccc accaacttaa aataagtgca ttctctgggg tgtgtgtctg aattaactta
    20641 gctgtctcca cccaaataga ttgccttaat gcagtggtat taggggtaag actgtcgtgt
    20701 gttcctgcgg gaaaaagtat ggcgttactt gcctgtgcgt tttggttttt ataattagca
    20761 accaataaac taaacgcatc tttaccattt ttctgaaagg tataccggct attctcatca
    20821 acaggaaaaa cacctcggag atcagatgtt ttttcctgag ttcgctttac catcaagggt
    20881 gcatctgcgt cctgcttcat gctactcagc ttatattgct tatatagtag cgtaccgcca
    20941 gctatcagca gtacgccact aaacaacacc agacaagctt gttttaaact tttgagcatg
    21001 gtgtcacttg tcacttagct tgcgtctttt tgagcggaag ctaagtaagc ttgaacgtaa
    21061 ggcaggaaag ccaaccaatg tacccgcaat cattaacagc aagcccccgc cacccaacat
    21121 attttcacta cccagttggc tcgcatcgct tactttaacc tctttaccag ctttagcggc
    21181 atctgctgct tcacgtaaac gcgtgtcttc atccataatt tcagcataac cacgcattaa
    21241 ttcagaccag ccttcgctat aagtatagcc acctggattc acatggaaaa ttcccttgta
    21301 atgtttaatg acatcatgtt cccacatgtc agcgtaaagt cgctctatcc ggcttgggtt
    21361 gttgcccttt gcccaaaata gctggaagaa tccaccagct gcatcttttt cgggtgcagg
    21421 tggggctggg cgattagttt tttgcccaac cagcaagccg tcttcatata gcttattcac
    21481 taccttcttg gcactttgct caacatttaa accttgaata gtacctttat ccgcggtatc
    21541 taaataagct ttggcaaagc tttctgagtg acacaaagta caggtttgca cccacgcctt
    21601 tttacgatct tcaaaccaag ggtgagaaag attattggca atttcaggcg tagggttgaa
    21661 accccagcgt actttacgca ctagattgtg tgaatactcg cctttatatt caaaatggca
    21721 agtctggcaa gttggtgcgg tataaccacc tttgccaaca gcatccttca aaggcacttc
    21781 aaaattccat ttggtcttgc ccatcgtcaa gtattgagta ccatgcttgg ataacatgta
    21841 gttttcaaac tcattatgat ccacaccatt gtgacaggtt gcacaagcct ctggttgtct
    21901 agcctctgcc gcagaaaaag tatggcgggt atggcaacca tcacatttat tttgttggta
    21961 atggcaagaa tcgcaacctt gtgcaacttc acgctcaggc atagcagccc acgttgcagt
    22021 ttcaacattc gcctcccaat caacggcatg ggaagggtgt cctttagccc actgcttttg
    22081 aggccactct tgctcttttt cagactcagc ttcagcaaat tcgtttaagt ggcaagtacc
    22141 acaagatacg cgatcaggca tgactagcgc tttagcatgg tcaatctttg taacacctac
    22201 tccaccatga caatcaatac aaccaacttg cttgagtggc tcaccttttt tcaacaaatt
    22261 taacttgcgt aaattatttt caacttccgt tattttttgt ttcttgtaag cacgagcgtc
    22321 actatcaggt aatttacgaa tagcttctaa atcactatgt gagctacttt tccatgcgtg
    22381 aaaagcacct ggcgttgttg tttggtggca ccccacacaa tcagactgcg taaattcacc
    22441 ttttatcgtt tggggaggcg agtaaaaatc ttttggattc cagtattttt gaatcgggat
    22501 aggctcccaa aatttgccgt ggctaccttt tccaggatgc gtatcgtaat aatttttctt
    22561 aatcttctca aagtcatgct ctacagccca agcggtattg ataagaccca atccaattaa
    22621 gctagctaaa aatgtagtta aaatccaccc tattagattt ttcatttcac caccccttaa
    22681 atttagaaca aaatttcaat taactaaaac tattgctaac tgagtgttgc gttatcacta
    22741 ttcaaaacaa tgactaattt ttatacttat gtgattagat accaatgcta ggattatggg
    22801 attacattca caacaaccag atttaaaaat ttaaatggtc gagtaagtta catatgtcaa
    22861 cggtattgaa tggtcgttgt agttatttaa atcctactta aaactggtat gcaaagaaga
    22921 tttacacgcc atgccgacac tatggagagt attgctgttg gaaatgcaaa cgaccactgt
    22981 cgtcgcggtg aaaacattcc gtaattggat gaagaaattt tagtgaattt agcgcgttaa
    23041 tttgatgata aaaaaacagg atgagatttt ggctttattt ttagaccaga ctaaattaga
    23101 agcgacttct gtgaatacat ttgtcaactt tctcacattt tgatttagta aattacagca
    23161 aaaaaaatag cactcaacta ggcgccaaaa tacacccaat tgggtgaaag acaaccccaa
    23221 tattgagtga tattgtgagt atactaaaat ttacaaatta ggaggtgtga gatgaatttt
    23281 aaaatagcta caattgggtt gttgtcttta tttgcagcgt ctgcacaagc ggcagtgatt
    23341 gggagcgatg caggtatcaa ggctgacatc gttgccagtg gcaacttcaa caacgcgggc
    23401 gacgatggca cgctaggttt atcctacaac ggtaaagagt ggattaattg gggaactcat
    23461 tcctcttgga cgtggttatc agcgtctaat ttagcatccc ccgttaacac attgggtggc
    23521 aacaacccac ttggtgcatc aattttggct tcaggctcaa atttcgttct gaatggtttt
    23581 tttgacggcc tgacatttat tcaaaccgca actcttaata acccaaacca aattggtttt
    23641 acgatttctt tgaccaataa tacaggcaaa gatattacgg acgtattttg gggcgtaggt
    23701 ctagacccag atattaatat cgcttttggt actagtaatt acgctacaat caacgccatt
    23761 acaggtcttg gtggtaatgc tgctgtcagc gcgtctggtt ctggtcaaac attgacttta
    23821 gcaaatacta ccagcgcttc agcctttaat attgcagcct atattaatgg tggtgattgt
    23881 tgtagccctg tcgacccagc agtcgcactt gcagctggtc aaatactagg ctattcaacc
    23941 aactccgatg attcaattag cttggcatac ggcttgggaa cgattggtat tggcaaaacc
    24001 gtgaccttcg gctacagcat tgctgcggtt ccagaacctg aaacttacgc cttaatgctt
    24061 gctggtttgg ggttaattgg ttttactgca cgtcgcagaa aaaactagct cgcaataaaa
    24121 ttagtctggt cgaaaggccc tgctaaattt agcagggctt ttttattgcg ccattcatca
    24181 agttataaat atttattgtc tcttaattct aaaattctca ctaaaatcat tttttagaaa
    24241 gaaattaata ttcacaaaag tacatctatg gtaagtaatt tgtagatggg aaactgaccg
    24301 agaaaaaatt actgtcctgc aagcctttat ttataaggct tgcaggacag ctctaatggt
    24361 tgcatcttcg gaagttgcat tcttttgact aagaactttc atttcaagat tattaatccc
    24421 atgcaaattg aatagttttg aatcgcatac tatacatata aataaaagct ttttgcacac
    24481 cctgcgacta gggtaaaata gcatttcatt cgaaccgttg ttttgaaagc tcgcacgatg
    24541 tcacttacca cgctcaacgc tctctctccc cttgatggcc gctaccaatc taagctagat
    24601 gcactacgcc cttactttag tgagtatgcg ctgataaaac accgagcttg ggttgaagtt
    24661 gagtggctaa aagcgctgtc agcggctaaa gatttacaag aaattgcgcc gtttagcgca
    24721 gaaacgatta aagagttaga tactacaata gccaacttta gtgaagttga tgcggcacaa
    24781 gtgaaagcca ttgaatcacg cacgaatcat gacgtaaaag cgcttgaata ctggctaaaa
    24841 gaaaagtttg atgccaaccc agaaatcaaa aaagccagcg aatttattca tttttcttgc
    24901 acatcagaag acattaacaa tttatcgcat ggcttaatgt taaaaactgc gcgcgatagc
    24961 gtaatgttgc catttttaaa caatttaatt gcacgtttaa gcgagttagc acaacaattg
    25021 gccgcacagc caatgctttc acgcacacat ggccaaaccg cttcccctac gaccatgggt
    25081 aaagagttgg ctaatgtggt ttttcgttta cagcgccaac aaaaacaatt taaaaacaat
    25141 gagatattag gaaaaatcaa cggtgctgtg ggtaacttta atgcgcactt gtcagcctac
    25201 ccaacgtttg attgggaaaa ttttgccaag cactttgtag aatcgctcgg attagcttat
    25261 aacccgatga ccatccagat tgagccgcac gattatatgg cagaactttt tgacaccatt
    25321 gcgcgcatca acaccatttt gattgacctc aaccgcgata tttggggtta tatttcagtc
    25381 ggctacttta agcaaaaggt taaagcgggt gaaattggct cgtctaccat gccgcataaa
    25441 gtgaatccga ttgactttga aaactcagaa ggtaacttag gtttggcgaa tgcagtgttg
    25501 cgccacatgg ctgaaaagtt gcctatttca cgctggcagc gcgatttaac ggattccacc
    25561 gtgttgcgta acatgggcgt ggcattcggc tacaccttgc tgggttttga ttcatgctta
    25621 cgcggcttaa acaaactcga aattaacgcg gcaaaactgg ctgaagattt agacaatagc
    25681 tgggaagtgc tcgctgaacc gattcaaacc gtgatgcgcc gctatggtat tgaaaaccct
    25741 tacgagcaac tcaaagaact cacccgtggc aaaggcggca tcaataaaga ttcactgcat
    25801 gtgtttatta gcggtttagc gattccgcat gatgccaaag ttgccctact tgccctcacg
    25861 ccagcgacct atattggcaa ggcaacggag cttgcgaagc atatttaatg ttgctgggat
    25921 gaagtcaaca ttgcaacact tcagcccaac aaccatgaat agccagattg ctgttattgg
    25981 ccggcttttt tttcagtcta tatttctact ggctttttta ctcacctgcc caacaacaca
    26041 ggctgcagag cttcaatatc aagttgaaat tattgttact gcagaaaacg cgaatggcca
    26101 ggtggccgca atcaaatcgc tgcttg
//
LOCUS       DAOQSI010000011        25698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_1191_length_25698_cov_5.143821, whole genome shotgun sequence.
ACCESSION   DAOQSI010000011 DAOQSI010000000
VERSION     DAOQSI010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 25698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25698
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1191_length_25698_cov_5.143821"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /gene="gloA"
                     /locus_tag="PKL42_01670"
     CDS             complement(<1..380)
                     /gene="gloA"
                     /locus_tag="PKL42_01670"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HNU65807.1"
                     /translation="MRMLHTMLRVGNLERSIKFYTEVLGMQVLRQHDYPDGKFTLAFV
                     GYGAEKDHTVLELTYNYGVESYDMGKAYGHIAIEVDDAYQACEAVKAAGGKVVREAGP
                     MMHGTTVIAFVEDPTNQDQRYTRNR"
     gene            complement(436..687)
                     /locus_tag="PKL42_01675"
     CDS             complement(436..687)
                     /locus_tag="PKL42_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HNU65808.1"
                     /translation="MALMITDECINCDVCEPECPNGAIYQGQEIYEIDPNKCTECVGH
                     YDKPQCQIVCPIDCIPLNPQVVETKEQLLQKFYKLTAEK"
     gene            complement(695..1207)
                     /gene="coaD"
                     /locus_tag="PKL42_01680"
     CDS             complement(695..1207)
                     /gene="coaD"
                     /locus_tag="PKL42_01680"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNU65809.1"
                     /translation="MTNTIFNAKTRIAVYPGTFDPITLGHEDLVRRAAHLFDEVIVAV
                     AGSTNKATLFTLEERVQLAKNVFADFSNVKVVGFSGLLMQFVQDQGAQMVIRGLRAAS
                     DFEYEFQLAGMNRKLYPQFETLFLTPSEQFMFVSSSLVREVAKLGGEVDQFVSPTVEA
                     AILQKLNQLE"
     gene            complement(1200..1745)
                     /gene="rsmD"
                     /locus_tag="PKL42_01685"
     CDS             complement(1200..1745)
                     /gene="rsmD"
                     /locus_tag="PKL42_01685"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNU65810.1"
                     /translation="MKSNTVRIGGGNWRSRLIKFSDSTGLRPTPDRVRQTLFNWLGQD
                     LTGKTCLDLFAGTAVLGFEALSRNAQQVVIVEKSPAVHQMVLQNQAALNAVSARIYKA
                     DALDFLKSNQQQFDVIFLDPPYNQGLLDKVLPMLPTHLAADGLVYAEAEYALKDSTVW
                     QVVKQSKAGNVFYHLLKLAHD"
     gene            complement(1762..3069)
                     /locus_tag="PKL42_01690"
     CDS             complement(1762..3069)
                     /locus_tag="PKL42_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNU65811.1"
                     /translation="MFRYLVFSLALMLSFSTNAAVKIDHWQTNSGARVYFVENHDLPI
                     VDLSVNFAAGSARDSVEKSGLAGITRYMMTLGAAGLSDEVLTNQFADVGAVLGGSFDA
                     DRASFKLRTLSSQAEQSQALDTFKKILHQPDFPQAVLAREKSRIIAGIQEAETQPESI
                     SNQAFMSALYGTHPYALDESGTVASVNAIGNQDLAAFYQQYYSAKGAVIAMIGDLTRE
                     QAMKIADEIADGLPKSATPAPIPAVSLPQAANVQKIAHPASQSHILIGYPGMKRIDPD
                     YFALYVGNYILGGGGFVSRLTEEVREKRGLVYSVYSYFMPMQEIGPFQIGLQTKKEQA
                     EDALKLVHETLDNFIKNGVTDKELQAAKANIIGGFPLRIDSNGKILDYLSVIGFYDLP
                     LTYLDDYNKKVAAVTIAQIKDAFSRRIDTQKLVTVIVGSPDAK"
     gene            complement(3206..4561)
                     /locus_tag="PKL42_01695"
     CDS             complement(3206..4561)
                     /locus_tag="PKL42_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNU65812.1"
                     /translation="MPIKKLLFLLLATPALALANTQEYKLENGLKIIVQEDHRSPVVV
                     SQVWYKAGALDEVNGKTGVAHVLEHMMFKGTKKVPAGQFSRKIAAAGGRENAFTSQDY
                     TAYFQQLEKSQLPLSFELEADRMANLNFTKEEFEKEIKVVMEERRWRTDDKPQAQVNE
                     HFNSAAFRTHPYGRPVVGWMSDLETMRVEDTREWYNHWYAPNNAVLVVVGDVKADEVY
                     KLAKQHFGKIKPKAVPERKPQKEPPQIGERRVVVKAPAKLPSLQIGFHTPVLNDPNQD
                     WEPYALEILASVLSGNDSARLNQKLVRESQVAVDVGAGYDMIARGRQGLFELAGSPSE
                     GKTVAELETAILQQIEKIKESGVTAEELKRVKAQVIAADVYQRDSVFYQAMQIGQLEL
                     TGFSWKILKDYPAKLSAVTSEQVQEVAKKYLVKDNMTVATLDPQPIDPNAKPKGKPHV
                     H"
     gene            4766..6028
                     /locus_tag="PKL42_01700"
     CDS             4766..6028
                     /locus_tag="PKL42_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNU65813.1"
                     /translation="MKFSPRLLPLFVFLASTHAYATDGYFAHGYGVKSQGIGGVGIAL
                     PQDALAAASNPAGLGLVGDRLDIGVTWFRPQRETKLENTTFGFKMFDGTYDANDTENF
                     IIPEFGYNKQINPDVTVGVSVYGNGGMNTDYKKAIPLLGTSPAGVDLVQLFVAPTVTW
                     KITPSNTIGASLNLAYQRFEATGLQNFDNPFFTKHPGKVTNKGHDSSTGFGLHLGWIG
                     QINDAVTLGATYQTKTRMSKFSDYKGLFAEGGDFDIPATYGVGLAVKATPALTLAADV
                     QRIEYSDVDSVGNSINNLLLKGNPLGSSKGPGFAWRDVTAVKIGALYAYNENLTLRAG
                     YNHSTQPIRKSETLFNILAPGVVQDHVTLGATYVLPNNGELSFAYMHAFEEKVKGNNN
                     SIPAGFGGGEANLKMYEDSLGVAYGWRF"
     gene            6097..6531
                     /locus_tag="PKL42_01705"
     CDS             6097..6531
                     /locus_tag="PKL42_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNU65814.1"
                     /translation="MTIDWLNFTPWSALAGGVLIGVSAALFILLNGRIAGITGIIGGL
                     FKPTKNDIGWRVAFTLGLIIAPLVWQSFQPLPSINIDANFTLLIIAGLIVGFAARYGS
                     GCTSGHGVCGIARLSPRSIVATLAFMGTGFLTVFIIRHLLGA"
     gene            6535..6975
                     /locus_tag="PKL42_01710"
     CDS             6535..6975
                     /locus_tag="PKL42_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNU65815.1"
                     /translation="MKRNLNHFSALLFALFSGLIFGIGLILAGMANPAKVLAFLDLAG
                     LWDPSLGLVMGGAIAVGLIAFTVAKKRTKSFLGLPMNLPNSRIIDKRLIIGSLIFGIG
                     WGLVGICPAPAFVLLGTGSLKGIVFLVAMLIGMAIFEYFDTRNK"
     gene            7056..8084
                     /gene="ftsY"
                     /locus_tag="PKL42_01715"
     CDS             7056..8084
                     /gene="ftsY"
                     /locus_tag="PKL42_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNU65816.1"
                     /translation="MFDFFKKKPPQPEAPQPEAPKAETSVVTTPVVEAPAAVEPTLSW
                     TERLKQGLSKTRKQLGSQLSGLFSGGKIDADVYEELETILLTSDVGVGATQFLLDDIK
                     GRVKRQNLDDTVQLKTALKDALADLLLPLQLPLETSTHKPFIIMLAGVNGAGKTTTIG
                     KLAKYYQAQGKSVLIAAGDTFRAAAREQLQVWGERNNVHVVAQSSGDPAAVMFDAINS
                     ARAKNIDVVLADTAGRLPTQLHLMDEIAKVKRVMDKALPGAPHEVLLVLDANTGQNAV
                     SQVKIFDDALGVTGLVLSKLDGTAKGGVIAAIAQARPIPIRFIGVGESIDDLRPFNAH
                     EFIDAMFE"
     gene            8385..9071
                     /gene="ftsE"
                     /locus_tag="PKL42_01720"
     CDS             8385..9071
                     /gene="ftsE"
                     /locus_tag="PKL42_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HNU65817.1"
                     /translation="MIKFDQVTKRYPGSNEVLKNASFTIEAGEFVFVTGHSGAGKSTL
                     LKLIAAIERPSSGTVLIANQNISQLKESAIPFMRRRFGLIFQDHKLLYDRNCFENVVL
                     PLHINGITGLEAAKRVRAALDKVGLLNKEKAMPITLSGGEQQRLAIARAVVSRPSILI
                     ADEPTGNLDANYAADIMAIFQAFHQVGVTVIMATHDALNRTATQNRLLHLDHGTLKQT
                     NVAATEQFTA"
     gene            9068..10003
                     /gene="ftsX"
                     /locus_tag="PKL42_01725"
     CDS             9068..10003
                     /gene="ftsX"
                     /locus_tag="PKL42_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNU65818.1"
                     /translation="MSKNMISTALNHHRQALKLVLGRMRQNALSTLMISLVIAAALCV
                     PGLFYLGVNHISSFANHIQDENEISVFLKLDADSSAIANVKDALNKNEAVQSVKFVSK
                     DEAWQQLRDKAEKDSASSNVINELKTNPLPDAFLVLAKSSKPEDLEALKDELKSLARV
                     DQALLNTEWVKRLSALLALGKQIIIIVAGLLAAALLIIISNTVRMQILTQKDEIIVSK
                     LIGATNSFIRKPFLYAGMLYGLFGGLLAIVMLVGFIMIFNQTLAQLSALYNSDFSLAL
                     INLPLFAGMVCAAIAIGWLGSYVAVTRAAATININ"
     gene            10204..10413
                     /locus_tag="PKL42_01730"
     CDS             10204..10413
                     /locus_tag="PKL42_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YezD family protein"
                     /protein_id="HNU65819.1"
                     /translation="MTIQASPKTNRDVSDDILRAIEELKSGTGFGSVEIIIHEGRVTQ
                     IEKREKVRFTLDKSKSSPTALAAAK"
     gene            10478..12079
                     /locus_tag="PKL42_01735"
     CDS             10478..12079
                     /locus_tag="PKL42_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNU65820.1"
                     /translation="MQFNYSKFATALIASGIISFPAIVHANDSTELEELRGLVQELSQ
                     QVKVLARKGEINEETAAADKKAAPVIKAGTSGFSFGSADGKNEIKFRGLLQADHRHYF
                     DGANDVRGRSNERAGTLNADGFHNAEDTSLLRRVRPTIEGKVAGKYGFRFTPEFAGGS
                     ASAVDAYVEANLNPAFKIRAGKQKSIVGLERLQGGGDIKFVERSYVTNAILPNRDLGV
                     TVSGDLLGNKLNYGVGIVNGVADGGNISTGAEYNGEREYTARIFATPFIDSDSALAGL
                     GFGIGGTWTDYTGEKNLNFTDTSAADATRNGLPSYVTEGQQTFFRYTGNAIADGQRIR
                     WSPQANYYNGPLGVIAEYAKVSQDVSLASGGSASAGGAGTSTVITPGTKKNLKHDAWE
                     IGVSYLLTGEDASFKGVKPKTDFDLDKGGWGAWELVARYSEINLDNDTFKNAAGAYAA
                     EGSAIGNSFADLTKSAKSAETWTLGVNWYLNTNAKFALNYARTSFDGGALQKGLAQTL
                     NNNATNRSRDREDEDALLARFQVAF"
     assembly_gap    12147..12246
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12400..13410
                     /locus_tag="PKL42_01740"
     CDS             12400..13410
                     /locus_tag="PKL42_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNU65821.1"
                     /translation="MKKTLLAIALFSAVFGVTAPSSAFAKDVEILNVSYDPTRELYVD
                     FNKAFAAHWKAKTGDNVSVKQSHGGAGKQARAVIDGLQADVVTLALAYDIDAIHEKAK
                     LIPKDWQARLPNNSSPYTSTIVLLVRKGNPKKIKDWDDLVKPGVSVITPNPKTSGGAR
                     WNYLAAWAYALKNNNNDEGKAKDFVKKLFANVPVLDSGARGSTTTFVERGIGDVFISW
                     ENEAFLALKELGPDKFEIVVPTLSILAEPPVTVVDKNAKKHGTEAVSKAYLEYLYSDE
                     GQDIAGKHYYRPTNPKYAEKYASQFPKVNLIKIDDVFGGWQKAQKTHFSDGGTFDQIY
                     QK"
     gene            13510..13848
                     /locus_tag="PKL42_01745"
     CDS             13510..13848
                     /locus_tag="PKL42_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HNU65822.1"
                     /translation="MRSVLIVGGDQIDGIKQVLSHHGVDHIHHWSGRKAGDGHKVIPQ
                     NTRLIVLITDWISHSFTYSIKRTAAKRGLKVIYTGNGAAQLQAKLEKIGNTLVDACRK
                     TINHLFLLPH"
     gene            13944..14159
                     /locus_tag="PKL42_01750"
     CDS             13944..14159
                     /locus_tag="PKL42_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65823.1"
                     /translation="MKNAFSSKISALFLSFILFEATVASIAFYEITEIKSAPVQAANK
                     ANNTSQKAQQKWDGYAPYALYQTLENL"
     gene            14403..15674
                     /locus_tag="PKL42_01755"
     CDS             14403..15674
                     /locus_tag="PKL42_01755"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HNU65824.1"
                     /translation="MKPETIAIHTGYSPEPTTKAVAVPIYQTTSYAFDNTQHGADLFD
                     LKVQGNIYTRIMNPTTAVLEQRLAALEGGIGALAVASGMAAITYAIQTIAEAGDNIVS
                     VSTLYGGTYNLFAHTLPKQGIEVRFFDYNDPTALEKLIDSKTKLVFAESIGNPLGNIV
                     DIAAISKIAHKHGVPVVVDNTVATPILHRPFEHGADIVVHSLTKYIGGHGNSIGGIII
                     DSGKFPWGEHKARFPVLNTPDPSYHGVNYVEALGPAAYIARARVVPLRNTGAALSPFN
                     TFLILQGLETLALRIERHSDNALRVAQYLKQHKKVKWVKYAGLSDHPEHALVHKYLSG
                     KASGILTFGVAHGIAGGTRFIDALKLITRLVNIGDAKSLACHPATTTHRQLNAEELKK
                     AGVSEDMVRISVGIENVEDIIEDLEQALSQV"
     gene            complement(15815..17710)
                     /locus_tag="PKL42_01760"
     CDS             complement(15815..17710)
                     /locus_tag="PKL42_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 2A encapsulin nanocompartment cargo
                     protein cysteine desulfurase"
                     /protein_id="HNU65825.1"
                     /translation="MTTNNGFSPALGDLSSFEPEAILAALPGEHPRMAAALAIGRQAS
                     ETGHFDVAELSRIANELYAKGFPAGSPFAQKEDFPSNASLNPVAANAVLPSPNIISDP
                     VQTPTPSIFSAVPSVASGVPNLSPTDVTNTGYTNQLPISGVFDIAQLLGGIDDPYQAE
                     LNQLLSGLDSQSGISSAGTNQKSSQAFSQTTSQAGAAPYYFLQDSPHQQDAAVAGPVA
                     SAHPPFDANLVRKDFPILRELVNGRPLIWFDNAATTQKPQAVIDRISYFYAHENSNIH
                     RAAHELAARATDAYEGARETVRRFINAPSVDNIVFVRGTTEAINLVAKTWGKKHIGEG
                     DEIVISHLEHHANIVPWQQLCQETGAKLKVAPVDDSGQVLLAEYQKLLTSRVKLVSFT
                     QVSNALGTVTPAAQMIQMAHAAGAKVLLDGAQSVSHMRTDVQALDPDFFVFSGHKVFG
                     PTGIGALYGKKEVLDDMPAWQGGGNMIQDVTFDKTVYHEAPAKFEAGTGNIADAVGIG
                     AALEYVERIGIDNIARYEHELLEYATAAMCKVPGLHLIGTAKEKASVLSFKLDGYESE
                     EVGAALNKEGIAVRSGHHCAQPILRRFGVETTVRPSLAFYNTTAEVDVMVSALLRLTS
                     HKAPGGL"
     gene            complement(17697..18614)
                     /locus_tag="PKL42_01765"
     CDS             complement(17697..18614)
                     /locus_tag="PKL42_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 2A encapsulin nanocompartment shell
                     protein"
                     /protein_id="HNU65826.1"
                     /translation="MTDIHQAHTALGDVAARTLSNATKTVPMLGTITPRWLVHLLQWV
                     PVEAGIYRVNKVKDPSAIEVDCSGKDEREIPQTFVDYEEWGREYVLNAVNTAVDVHTR
                     VSDLYSSPHNQIKEQLRLTIETVKERQESELINNKEYGLLNNIADSQKVKSRTGAPTP
                     DDFDELLTKVWKEPAFFLAHPQAIAAFGRECTRRGVPPPTVALFGSQFITWRGVPIIP
                     CDKLRVTKGKTNILLLRTGESRQGVVGLYQPNLPGQQSMGLSVRFMGINHKAIASYLV
                     SLYCSLAVLTDDAIAVLENVDVGNYYDYK"
     gene            complement(18648..19595)
                     /locus_tag="PKL42_01770"
     CDS             complement(18648..19595)
                     /locus_tag="PKL42_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HNU65827.1"
                     /translation="MTNQTATLTNEDFDISGIVQALRDVRLTSLEHRHRKNKPPKLPS
                     RKALQSVVDGLSAALFPNRLGAPGLKDEGIDYYVGFTLDTALRELNTQIQLELRYSAD
                     TGHDKTPSVEAALNKARAFAKTLPKIRQLIDTDLNAAFEGDPAARSLDEVLVCYPGIT
                     AIIHYRLAHELHTLGLPLIARIISELAHSATGIDIHPGATIGEGFFIDHGTGVVIGET
                     AVIGNHVRLYQAVTLGAKRFPTDEHGHLIKGSARHPILEDDVVIYAGATVLGRITIGA
                     RSVIGGNVWLTRSVPPDSQITQAQSQASIQETVAVENTK"
     gene            19821..20837
                     /locus_tag="PKL42_01775"
     CDS             19821..20837
                     /locus_tag="PKL42_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNU65828.1"
                     /translation="MHIQTIIHQLKLTAVALAGAFVFSNSASAETSLLNVSYDVSREY
                     YKDLNPAFIQYWKEKTGETITINQSHGGSSKQARSVVDGLSADVITMNRSPDIDILYE
                     RAKLTPKDWQKRLPHASSPTASTSVFLVRKGNPKNIKDWDDLVKPKVAVVIPNPKTSG
                     NGKYTYLAAWGYVLKNGGDEAKAKEFVTKLFKNVPVLDTGGRGATTTFAQRDIGDVLL
                     TFENEVFLIQKELGADKFEVIYPSISVLAENPVALVDKVVDKRKTRTQAQAYLEFHFS
                     EVGQDIAAKHYLRPQVDSAVTKHAATFKPLKLFKVEEVFGSWSKAEKTHFADGGVFDQ
                     IYAK"
     gene            20889..21779
                     /locus_tag="PKL42_01780"
     CDS             20889..21779
                     /locus_tag="PKL42_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNU65829.1"
                     /translation="MTKHQQQQASLALLKEQLKESLQLDLDDFALEANALHEYVSKFV
                     GINLKSSFQPIYDIEKGDLHGFEASLLASLGDIQETTADFAYSYAKASGKLVKFDRVV
                     RTLHLLNYKQIFQEKGLLFLSVHPDLLISVNEHGKVFERILHQYSLPTERVVIQIRDY
                     STTEQIENLVTYERQLAAAIENYHDRGYKIAIDYFGNKHSLVSRLWKISPDYIKFDPS
                     LIQEAESNVRLRTTLKNLTDLIKDLDTTPILTNVVTQAQLDIALHAGISFVQGDYLGK
                     SSSSSQLHASELIQKRFVAS"
     gene            21944..22771
                     /gene="cysT"
                     /locus_tag="PKL42_01785"
     CDS             21944..22771
                     /gene="cysT"
                     /locus_tag="PKL42_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HNU65830.1"
                     /translation="MAKKSSLPGFNLSLGYTIFYLSLIVLIPLSAVFFKTFTLSWDEF
                     VQAVASPRVVASYKLTFGASLIAASINVVFGVLTAWVLTRYEFFGKKIVDAFVDFPFA
                     LPTAVAGISLSAIYAKNGWIGSLLDQVGIAVAYTPLGVVVALTFIGLPFVVRTVQPVL
                     EDLGAEVEEAGASLGANRWQVFYKIILPAIWPAVITGFALAFARAVGEYGSVIFIAGN
                     MPMVSEITPLIIITKLEQYDYAGATAVAAVMLVISFILLFAINGFQWWSNRRLGHVK"
     gene            22790..23698
                     /gene="cysW"
                     /locus_tag="PKL42_01790"
     CDS             22790..23698
                     /gene="cysW"
                     /locus_tag="PKL42_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HNU65831.1"
                     /translation="MAAANAHSLSQYAKYHNKVGRSRATSEAAWVRYTLIGVALLFLC
                     LFLFIPLAAVFVEAFRKGAETYFASLTEADALSAMKLTLIAAAISVPLNLVFGVSAAW
                     AISKFKFKGKSFLITLIDLPFAISPVIAGLIYVLIFGLQGWFGEWLSDHDFKIIFAVP
                     GIVLATIFVTFPFIARELIPLMQAQGKDEEEAAMVLGASGWQMFWHVTLPNIKWGLLY
                     GVILCNARAMGEFGAVSVVSGHIRGLTNTMPLHVEILYNEYNYAAAFAVASVLALLAL
                     VTLALKTYVEWQAARNAEETEREITE"
     gene            23754..24809
                     /locus_tag="PKL42_01795"
     CDS             23754..24809
                     /locus_tag="PKL42_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HNU65832.1"
                     /translation="MSIEIKNINKGFGSFSALNNVSLEVPTGELVALLGPSGCGKTTL
                     LRIIAGLETPDTGSIHFYGKDATERAVREREVGFVFQHYALFRHMTVFENVAFGLRVR
                     PKHTRPNDAEIKRRVHELLNLVQLDWLADRYPPQLSGGQRQRIALARALAVEPKVLLL
                     DEPFGALDAKVRKELRRWLRRLHDELHVTSVFVTHDQEEALEVADTIVVMNQGKIEQI
                     GSPQQVYDNPATPFVYQFLGNVNAFEGRVNAGVAKLGALEVPVKEFAQTHDSAVQAFA
                     RPHDIDISRDASAAAFKAQLTYVHSAGSVVRLELKRDDAETFIEAELTREYFSQLNLK
                     EGETVYLTPRNVRFFLD"
     gene            25193..25630
                     /locus_tag="PKL42_01800"
     CDS             25193..25630
                     /locus_tag="PKL42_01800"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HNU65833.1"
                     /translation="MQYLIEYGYIGIFIAAFLAATILPLSSDVVLSALLLSELSPFTL
                     IAIATFGNVLGSLTNYALGYWASQKVIKRWLRMSDSDFTKAQQRFTKYGLLSLCFAWV
                     PIIGDPLTVIAGVLRIRLIWFLVLVTTGKLLRYLAVSYFVLFA"
BASE COUNT         7041 a         5773 c         5589 g         7195 t
ORIGIN      
        1 cggttgcgcg tgtagcgctg atcctggttg gtggggtctt ccacaaacgc aatgacggtg
       61 gtgccatgca tcatcgggcc cgcttcgcgc accacttttc cgcctgccgc tttaaccgcc
      121 tcacaggctt ggtaggcatc atccacttca atcgcgatgt ggccgtaggc tttgcccatg
      181 tcatagcttt ctacaccgta gttataagtg agttcaagca ctgtgtggtc tttttctgcg
      241 ccatagccaa caaacgctaa ggtgaatttt ccatcgggat aatcatgctg acgcaacact
      301 tgcataccaa gcacttcggt ataaaactta attgaacgct ctaaattgcc cacgcggagc
      361 atggtgtgca gcatacgcat tttgttatcc ttttattgtt gtgtatgaac gcaatggatt
      421 gcgctcctac aatgattatt tctctgccgt taatttgtaa aatttttgca gcaattgctc
      481 tttggtttca accacttgcg ggtttaacgg aatgcagtca attgggcaga caatctggca
      541 ttgcggttta tcgtaatggc ctacgcattc ggtacattta ttcgggtcaa tttcgtaaat
      601 ttcctgccct tgataaatcg cgccattggg gcactccggc tcacaaacgt cgcaattgat
      661 gcattcatcg gtaatcatta acgccatttg acgattactc taattggttt aatttttgca
      721 aaatagcggc ttctacagtg ggtgatacaa attgatcaac ctcgccacca agcttggcca
      781 cttcacgtac aaggcttgat gacacaaaca taaactgttc agaaggcgtt aaaaacaatg
      841 tttcaaactg cggatagagt ttgcggttca tgcctgctaa ttgaaattca tattcaaaat
      901 cagatgctgc gcgtaaaccg cgaatcacca tctgtgcgcc ttggtcttgg acaaattgca
      961 tgagcaagcc tgaaaaccca accactttga cgtttgaaaa atcagcaaaa acattttttg
     1021 caagttgcac acgctcttct aaagtaaaaa gcgtggcttt attggtgctg cccgccaccg
     1081 ccacaatcac ttcatcaaat aaatgggccg cacgccgtac caaatcttca tgccccagcg
     1141 taatggggtc aaaagtaccg gggtaaacgg cgatgcgcgt ttttgcatta aaaatagtgt
     1201 tagtcatgcg ctaattttag cagatgataa aaaacattgc ctgctttgct ttgtttaacc
     1261 acttgccaca cggtggaatc tttaagtgca tactcagcct cagcatacac cagcccgtca
     1321 gcggctaaat gtgttggcaa cattggcaaa actttgtcca gcaaaccttg attgtaggga
     1381 ggatctaaaa aaatcacatc aaattgctgt tggtttgatt ttaaaaagtc gagtgcgtca
     1441 gctttataaa tgcgggcgct tactgcattt aatgccgctt ggttttgtaa caccatctga
     1501 tgcactgcag gcgatttttc aacgatcaca acctgctgcg cattacgcga gagtgcctca
     1561 aaccccagca cggcggtacc tgcaaataaa tccaggcaag ttttacctgt taaatcttgc
     1621 cccagccaat taaataaagt ttggcgcacg cggtctggcg tagggcgcaa gccggtgcta
     1681 tcagaaaact taatcaggcg gctgcgccaa ttgccgccgc caatgcgcac cgtatttgat
     1741 ttcattgatc ttaaatctta tttattttgc gtctgggctg cctacaatca ctgtcaccag
     1801 tttttgcgta tcaatacgac gactaaacgc atctttgatt tgcgcaatcg tcactgcggc
     1861 tacttttttg ttgtaatcat ctaaataagt gagcggcagg tcataaaaac ctatcacact
     1921 caagtaatct aaaattttac cattactatc aattcgtagt ggaaaaccgc caatgatatt
     1981 ggcttttgcc gcttgcaact ctttatcggt gaccccgttt ttaataaagt tatccagtgt
     2041 ttcatgcact aatttaaggg catcttcagc ctgctctttt ttggtttgca ggccaatctg
     2101 aaacgggccg atttcttgca tgggcataaa ataactgtac acgctgtaga ctaaaccacg
     2161 tttttcacgc acttcttcag tcaagcgcga cacaaacccc ccgccgccca atatatagtt
     2221 acccacataa agtgcaaagt aatcagggtc aatgcgcttc atgccagggt agccaattaa
     2281 aatatgtgac tgtgaagctg gatgcgcaat tttctgtacg ttagcggctt gcggtaaact
     2341 cacagcggga ataggcgctg gcgtggccga tttaggcaaa ccatcggcaa tttcatcggc
     2401 aattttcatt gcttgctcac gcgttaaatc gcctatcatg gcaatcacag ccccttttgc
     2461 actgtaatat tgctgataaa aagccgccaa atcttgatta ccaatggcgt tcacacttgc
     2521 caccgtgccg ctttcatcaa gcgcgtaggg gtgcgtgcca tataaagccg acataaacgc
     2581 ttggtttgaa atgctttctg gttgtgtttc ggcttcttga atacccgcga taatgcgcga
     2641 tttctcacgc gctaacaccg cttgtggaaa atcaggctga tgcaaaatct ttttaaaagt
     2701 atccaaagcc tgactttgct cagcttggct acttaacgtg cgaagcttaa aactggcacg
     2761 atcagcatca aaactaccac ccaacactgc accaacgtct gcaaattgat tggtcagcac
     2821 ttcatcactt aggcccgctg caccgagcgt catcatataa cgtgtaatac ccgccaagcc
     2881 ggacttttca accgaatcgc gcgcactgcc tgcggcaaaa ttcacactca aatccacaat
     2941 tggcaaatca tggttttcaa caaaatacac ccgtgcgcca gaatttgttt gccaatggtc
     3001 aattttaacg gcggcgttgg tggaaaaact taacattaaa gccaatgaaa aaactaagta
     3061 tctaaacatt tttttctctt ttaaaatctg ctagttatcg tgaatgaaaa tacaaagctt
     3121 taagtcagac taggcgtgga aagccgacgt gtacgcgttg tactcgaggc tttttacaac
     3181 aacgtatgac gacaaggttt gcattttaat gtacgtgtgg tttgcctttg ggcttggcat
     3241 tcggatcaat cggttgcggg tcaagcgtag caacggtcat gttatctttt accaagtatt
     3301 ttttggccac ttcctgcact tgttctgaag ttaccgcact caattttgcg gggtagtctt
     3361 tcaatatctt ccaagaaaac cccgtgagtt ctaactggcc aatttgcatg gcttggtaaa
     3421 atacagagtc gcgttgatat acatcggccg caatcacttg tgctttaacg cgtttaagct
     3481 cttctgccgt gacgcccgat tctttgattt tttcaatttg ctgcaaaatt gcagtctcta
     3541 attctgcaac ggtttttccc tctgaaggcg aaccggcaag ctcaaacaag ccttgcctgc
     3601 cgcgtgcaat catgtcgtaa cctgcgccta catccaccgc aacttgcgat tcacgtacga
     3661 gcttttgatt gagtcgtgca gaatcattgc cactgagcac actcgctaaa atctctaatg
     3721 cgtacggctc ccagtcttga tttgggtcgt ttagcacggg cgtatgaaat cctatttgca
     3781 gtgacggtaa tttagcaggc gcttttacta ctacacgtcg ttcaccaatt tgcggcggtt
     3841 ctttttgtgg ttttcgctcg ggcaccgcct tgggcttaat cttgccaaaa tgttgtttgg
     3901 caagcttata cacttcatcg gcttttacat cacccaccac caccaacacc gcattattag
     3961 gtgcatacca atggttgtac cattcacgcg tatcttcaac ccgcatggtt tcgaggtcac
     4021 tcatccaacc caccacgggg cgcccatagg ggtgggttcg aaatgcagcg gaattaaaat
     4081 gttcattcac ttgtgcttgc ggtttgtcat cggtacgcca gcgacgctct tccatcacca
     4141 ctttgatttc tttttcaaat tcttctttgg tgaagtttaa attcgccata cgatcggctt
     4201 ctaattcaaa cgaaagcggt aattgcgatt tttctaactg ttgaaagtaa gcggtgtaat
     4261 cttggctggt aaaggcgttt tctctaccgc ctgcagcagc aatcttgcgc gaaaactgcc
     4321 cagccggcac tttttttgtg cctttaaaca tcatgtgttc caacacatgt gcgacaccgg
     4381 ttttaccatt cacttcatca agtgcaccgg ctttatacca aacttgtgaa actaccacgg
     4441 gcgaacggtg atcttcttgc acaataattt ttagaccatt ttctaatttg tattcttgtg
     4501 tattggccaa cgccaaggct ggcgttgcaa gcaataaaaa tagcaatttt tttataggca
     4561 tacgtttttt ctcttgatat tgaagtttgc taaattaatt taacgttctt ttattcaaat
     4621 gaacgctgtt gtgaccaatc aagcaccgtg caagttcaca cttctctata taactaattg
     4681 gcttaaatat atactcaaat attattgtga cttaattata aactttgcta tattgcagcc
     4741 catacaaaac cacttggaat atattgtgaa atttagccct agattattac ctttatttgt
     4801 ttttttagcc agtacgcacg cctatgctac cgatggctat tttgcgcatg gctatggcgt
     4861 taaatctcaa ggtattggtg gtgttggcat tgctttgccg caagatgcgc tagctgcggc
     4921 cagcaaccca gccggtcttg gcttggttgg agaccgcctt gacattggag taacgtggtt
     4981 tagaccgcaa cgtgaaacga agctggaaaa taccactttt ggttttaaaa tgtttgacgg
     5041 cacatatgat gccaatgaca ctgaaaattt tattattcca gaatttggct ataacaaaca
     5101 aattaaccct gacgtcaccg ttggtgtgag tgtttatggc aatggtggca tgaatactga
     5161 ctacaaaaaa gcaattccat tactcggcac gtcgccagct ggcgttgatt tagttcagct
     5221 ttttgttgcc ccaaccgtta cttggaaaat aacaccttca aacactatcg gcgcatcact
     5281 aaatttagca taccaacgtt ttgaagcaac aggcttgcaa aattttgata atccattttt
     5341 caccaagcac ccaggcaagg tcactaacaa aggacacgac tcatcaacgg gctttggcct
     5401 acatcttggt tggatagggc aaatcaacga tgctgtcacc ttgggtgcca cctaccaaac
     5461 caaaacacgg atgagtaaat ttagtgacta caaaggctta ttcgcagaag gcggtgattt
     5521 tgatattcca gccacttacg gtgtagggtt ggcagtcaaa gcgactcccg cgttaacctt
     5581 ggcggctgat gttcaacgta ttgaatacag tgatgtggat tcggtgggca attcaatcaa
     5641 caatctatta cttaaaggca acccacttgg ctcaagcaaa ggtccaggct ttgcttggcg
     5701 tgatgttact gccgtgaaaa taggtgcgct ttatgcctac aatgaaaatc taacattgcg
     5761 tgctggctat aaccactcca cgcaacccat tcgtaaaagt gagaccttgt ttaacatctt
     5821 agcccctggc gttgtacaag accatgtgac attgggcgca acgtatgtgt tgccgaataa
     5881 tggcgagtta tcttttgcct acatgcacgc atttgaagaa aaagttaaag gcaacaataa
     5941 ttccattcca gccggttttg gtggcggcga agctaaccta aaaatgtatg aagattcact
     6001 cggcgttgct tatggctggc gtttctaagt taaaataacc acgcatattt gccatacgca
     6061 gcatgcgtat ggctttttct tttttggagt attcatttga caatagactg gcttaatttt
     6121 acaccttggt cagcattagc tgggggtgta ctcatcggtg tttcagcagc actttttatt
     6181 ctgttgaacg gccgtattgc aggtattacc ggcattattg gtggcctgtt caaaccgact
     6241 aaaaatgata tcggctggcg agttgcattt acgcttggtc tgattattgc cccgctggtt
     6301 tggcaaagct ttcaaccact ccctagcatc aacatagatg caaatttcac cttgttaatc
     6361 attgcggggc tgattgtagg tttcgcggca cgttatggct caggctgcac aagcgggcac
     6421 ggcgtgtgcg gcatcgccag attatcccct cgttcaatcg tagccacgct cgcattcatg
     6481 ggcactggat ttttaacggt gtttatcata cggcatttgc taggagcata aaaaatgaaa
     6541 aggaacttga atcatttctc cgccctattg tttgcacttt tttctggcct tatttttggc
     6601 attggtctca ttttggctgg catggcaaac cctgccaaag tgctggcatt tcttgacctt
     6661 gctggcttat gggacccttc tctggggttg gtgatgggcg gggcgatagc ggttggttta
     6721 atcgctttca cagtggctaa aaaacgcact aaaagctttt tagggttgcc catgaatctg
     6781 ccaaactctc gcatcattga taaacgcctg attattggta gcttaatatt tggtattggc
     6841 tgggggctgg ttggcatttg ccctgctccg gcatttgttt tattaggcac aggtagccta
     6901 aaaggcattg tttttcttgt cgccatgtta atcggcatgg caatttttga atattttgat
     6961 acccgcaaca agtaaagacc cttagttact ttaataggca gcgcaaaact aggctaaaat
     7021 agtgcctatt caatcgccga attttaaaat taactatgtt tgattttttc aaaaaaaaac
     7081 caccacaacc tgaggcgccg caacctgaag caccaaaagc tgaaacttca gtagtcacaa
     7141 cgccagtggt ggaagcacct gccgcagttg aacctaccct gagttggacc gagcgtttaa
     7201 aacaaggctt gagcaaaacc cgcaaacaat tgggttcaca gctttcgggc ctttttagtg
     7261 gcggcaaaat tgatgcagac gtctatgaag aactagaaac cattttgctc acttcagacg
     7321 tgggcgtagg cgcgacgcaa tttttgctgg atgacatcaa aggccgtgtg aaacggcaaa
     7381 atttagatga caccgtgcaa ctcaaaactg ctcttaaaga cgcgctggca gatttacttt
     7441 tacccctgca actgccgcta gaaaccagta cccacaaacc ttttattatt atgttagctg
     7501 gcgtgaatgg tgctggcaaa accaccacca tcggcaaact ggccaaatat tatcaggcgc
     7561 aaggcaaaag cgtgctaatt gccgcaggcg acacctttag agccgccgcg cgtgagcaat
     7621 tacaagtttg gggcgaacgc aataacgtac atgtggtggc gcaaagcagt ggtgaccccg
     7681 ccgccgtgat gtttgatgcc attaactcgg cacgcgcaaa aaatattgat gtggtattag
     7741 ccgataccgc aggccgcctg cccacgcagc tgcacttaat ggacgaaatt gccaaagtta
     7801 aacgcgtgat ggacaaagcg ctaccgggcg caccacatga ggtactattg gtgctcgatg
     7861 ccaatactgg ccaaaatgcg gtttctcaag tcaaaatttt tgatgatgca ctgggcgtga
     7921 ctggcttggt attaagcaaa ctggacggca ctgccaaagg cggcgtcatt gctgccatcg
     7981 cgcaagcccg ccctattcca attcgcttta ttggcgtggg tgaaagtatt gacgatttac
     8041 ggccttttaa cgcgcatgaa tttattgatg cgatgtttga ataaaatgtt tgattaaaat
     8101 ttctgttaaa aagccttgcg caaacagtga ataatttgca ttttattctg caattaaaat
     8161 catcaaaata ggcttttata caccgaaacc tttttcgatg ctctggaagc cttataaata
     8221 aaggcttgca gagcataaaa aaatcctcgg agtaaattaa gctgattttt gcaaaaatat
     8281 tcgcgcgcaa tttgctattt gcagcaccca aagtacgcgg caatttttga gcgctacaca
     8341 gatttagcgc tataatcttc tgcaatataa agtagtatct cagcatgatt aaatttgacc
     8401 aagtgaccaa acgctaccct ggtagcaatg aagttttaaa aaacgccagc tttacgattg
     8461 aagcgggaga atttgtgttt gttacgggac attcaggtgc gggtaaaagt acgctgctta
     8521 aactcattgc ggccattgag cgcccttcca gcggcacggt attgattgcc aatcaaaata
     8581 tcagccaatt gaaagaatca gccattcctt ttatgcgccg gcgctttggc ttgatttttc
     8641 aagaccataa actgctttat gaccgcaact gctttgaaaa tgtcgtgttg cccttgcaca
     8701 ttaacggcat tacagggctt gaggcggcca agcgtgtacg cgcagcgctc gataaagttg
     8761 gtttgctcaa taaagaaaaa gccatgccga ttacattatc tggcggcgag cagcaacggc
     8821 ttgcgattgc ccgcgccgtg gtaagcagac cttctatttt gattgccgat gagccgaccg
     8881 gcaatttaga tgccaattat gcggcagata ttatggcgat ttttcaggcg tttcatcaag
     8941 tgggcgtgac ggtgattatg gccacacacg atgcacttaa tcgcaccgca acacaaaatc
     9001 ggctgctgca tttagaccac ggcaccttaa agcaaactaa cgttgcagcc accgagcagt
     9061 tcactgcatg agcaaaaata tgatcagcac cgcacttaac caccatcgcc aagccctcaa
     9121 actagtattg ggccgcatgc gccaaaatgc gctatcaacc ttgatgatta gtttggtgat
     9181 tgccgctgca ctgtgtgtgc cgggtttgtt ttacttgggt gtgaatcata tttcatcatt
     9241 tgcgaatcat attcaagatg aaaatgaaat cagtgtattt ttaaagctcg atgccgacag
     9301 ctcggccatt gcaaacgtga aagacgcgct taacaaaaat gaagccgttc aatcggtaaa
     9361 atttgtgtct aaagatgaag cgtggcagca attgcgcgac aaagctgaaa aagacagtgc
     9421 gtcaagcaac gtcatcaatg agctcaaaac aaatccattg cccgatgcct ttttggtact
     9481 tgcaaaatca tccaagccag aagaccttga agcgcttaaa gatgagctta agtcattagc
     9541 acgcgttgac caagcgctgt taaataccga atgggtaaaa agactgtctg ccctgctggc
     9601 tttaggcaag caaatcatta ttattgttgc cggcttactg gctgcggcct tgctcattat
     9661 catcagcaac acggtgcgca tgcaaatttt gacgcaaaaa gatgaaatta tcgtcagtaa
     9721 actgattggc gccaccaata gctttattcg taagcctttt ctatacgctg gcatgttata
     9781 cggtctattt ggcgggctac tcgccatagt catgctcgtg ggttttatca tgatattcaa
     9841 ccaaacgctt gcgcagttat ccgcgctcta caatagcgat tttagccttg cgctcatcaa
     9901 tctgccgctg tttgccggca tggtttgtgc cgctattgcc ataggctggc taggctcata
     9961 cgtggcagtt acccgtgcgg cagccactat taacatcaac taacccgtac tttcatttaa
    10021 ttgccaaaaa aaagatatcc atgtggatat ctttttttgt ttctactccg tagttcaaac
    10081 gctaagccct ttctcatata ttctttagct atattcatat aataactaat tctttttaat
    10141 tgttataaag tcgctatata gtgcctgagt tgctaaatat ttgcacagaa acgggcctta
    10201 atcatgacaa ttcaagcttc accaaaaacc aatcgcgatg tttcagatga catattacgt
    10261 gccattgaag aattgaaatc tggcactgga ttcggttcag tagaaatcat tattcacgaa
    10321 ggtcgtgtta cgcaaattga aaagcgggaa aaagtcagat tcacactaga caaatcaaaa
    10381 tcttcaccta ctgcactggc agcggcgaaa taaataaaat taaatttata tacattggct
    10441 gaccagtttg ctggaagcta gaatcaagga gaaaaaaatg caattcaatt attcgaaatt
    10501 tgccaccgca ctcatcgcca gcggcattat ttcattcccg gccatcgttc acgcgaacga
    10561 cagcaccgag cttgaagaac tacgcggctt agtgcaggag ttaagccaac aagtgaaagt
    10621 tttggcccgt aaaggtgaaa ttaacgaaga aacagccgcc gccgataaaa aagctgcgcc
    10681 agtgattaaa gccggtacga gtgggttctc ttttggttca gccgatggta aaaatgaaat
    10741 taaattccgc ggcttattac aagctgacca tcgacactat tttgatggtg cgaatgatgt
    10801 gcgtggccgc agcaatgagc gtgcagggac attgaacgct gatggctttc ataatgctga
    10861 agatacttca cttttacgcc gcgttcgccc aacgattgag ggcaaagtgg ctggtaaata
    10921 cggtttccgc tttacgccag aatttgccgg tggcagtgct tcagcagtag acgcttatgt
    10981 tgaagctaac ttaaacccag cattcaaaat tcgtgcgggt aaacaaaaaa gtattgttgg
    11041 cctagaacgc ttacaaggtg gcggtgacat taaatttgtt gaacgcagct atgtgaccaa
    11101 tgcaatttta ccgaatcgtg atttaggtgt aaccgtttct ggtgatttat taggcaataa
    11161 attgaactac ggcgtgggga ttgtcaatgg cgttgcggat ggtggcaaca tcagtacagg
    11221 tgcagaatac aatggcgaac gtgaatatac tgcgcgtatt tttgctacgc catttattga
    11281 ttcagactca gcccttgctg gtttaggttt tggtattggt ggcacatgga ctgattatac
    11341 tggcgaaaaa aatctaaact tcacggatac ttctgctgcc gatgcaaccc gtaacggcct
    11401 gccaagttac gtgacagaag gtcagcaaac attcttccgc tacacgggca atgcaattgc
    11461 cgatggtcag cgtattcgtt ggtcaccaca ggccaattac tataatggcc ctttaggcgt
    11521 gattgctgaa tatgcaaaag tatcgcaaga cgtgagcttg gcaagcggcg gctctgcttc
    11581 agcaggcggt gctggaactt caactgtgat tacaccgggt actaagaaaa atcttaagca
    11641 tgatgcttgg gaaatcggtg tttcttatct attaactggt gaagatgcgt cttttaaagg
    11701 cgttaaacca aaaaccgact ttgatttaga taaaggcggt tggggtgcct gggagttagt
    11761 tgcacgctac agtgaaatca atcttgataa cgacactttc aaaaatgcag ccggtgccta
    11821 tgcagctgaa ggaagcgcta ttggaaactc attcgcagat ttaacgaaaa gcgctaagtc
    11881 agctgaaact tggacgcttg gtgtgaactg gtacttaaac actaacgcaa aatttgcact
    11941 taattatgcg cgcacgtctt ttgatggtgg tgcgttgcaa aaaggcttag cgcaaacgct
    12001 aaataacaac gctacaaaca gatcacgtga ccgtgaagat gaagatgcat tgttagcacg
    12061 tttccaagtt gcgttctaac aataagttgc tttctaacgg cttgaatttt agtaaatcgc
    12121 ttatcccaaa tgctcaaaca tgcgggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12241 nnnnnnttct aacggcttga attttagtaa atcgcttatc ccaaatgctc aaacatgcgg
    12301 gataagttaa atttaaatag attaaaaaaa catgatgatg aaatgtattt aagcaacatc
    12361 aagaaaacat ttacacatta aattattgaa ggatttctca tgaaaaaaac acttttagcg
    12421 atcgcattat tttctgcggt gtttggcgta acggcgccat ctagcgcatt tgcaaaagac
    12481 gttgaaattt taaacgtatc atatgaccca acacgcgagt tgtatgttga ttttaacaaa
    12541 gcctttgctg cccattggaa agcaaaaacg ggtgacaacg tcagcgttaa acaatcacat
    12601 ggtggcgctg gtaaacaagc ccgtgcggtg attgatggct tacaagcgga cgtcgttaca
    12661 cttgctttgg cttatgacat tgacgctatt cacgaaaaag ccaagttaat tcctaaagat
    12721 tggcaggcac gtttaccaaa taacagttca ccttacactt caaccattgt gctgctggtg
    12781 cgtaaaggca accctaagaa aatcaaagat tgggatgatt tagtaaaacc aggtgtttct
    12841 gtgattacac ccaacccaaa aacgtctggt ggcgcacgct ggaattatct ggctgcctgg
    12901 gcttacgcac ttaaaaataa caataatgac gaaggtaaag ccaaagattt cgtgaagaaa
    12961 ctgtttgcca atgtgccggt gcttgactct ggtgcgcgtg gatcaaccac aacatttgtt
    13021 gagcgtggta ttggtgacgt gtttatttca tgggaaaacg aagcattctt agcgttaaaa
    13081 gaactaggcc ctgataaatt tgaaattgtt gtacctacac tatcaatttt ggctgagcca
    13141 cccgtgactg tggttgataa aaatgccaaa aaacatggca ctgaagcggt atcaaaagct
    13201 tacttagagt atttgtactc tgacgaaggc caagacattg caggcaagca ttactaccgc
    13261 ccaaccaacc ctaaatatgc tgaaaaatat gcgagtcaat ttccaaaagt gaatttgatt
    13321 aaaattgatg atgtatttgg cggctggcaa aaagcgcaga aaacacactt ttctgacggc
    13381 ggcacatttg accaaattta tcaaaaataa ccagaaataa cttacttagt taaatagtta
    13441 ttccgttaaa ttgttacccc tcagcatcaa acaaaatttg atgctgagtt tttacaggag
    13501 gcgcttacca tgcgttcagt tttaatcgtt ggcggtgacc agattgatgg catcaaacaa
    13561 gtattaagtc atcatggcgt agatcatatt catcattggt ctggccgaaa agcaggtgac
    13621 ggtcataaag tcattccaca aaacacacgt ttaatcgtat tgattaccga ctggattagc
    13681 cactctttta cctacagcat taaacgtacg gcggccaaac gtggcttaaa agtcatctat
    13741 accggcaacg gtgctgcaca acttcaggcc aaacttgaaa aaataggcaa tacattggtg
    13801 gatgcctgtc gaaagacaat caaccatcta tttttattac cgcattgatc tattccaccc
    13861 tcggctgcac accaaagcgc cattttgaaa cacagcggct atcgcgaata gatcctaact
    13921 tatttcaaat aaggagccta atcatgaaaa acgcgtttag tagcaaaata tctgccctat
    13981 ttttaagctt tattctcttt gaagccaccg tggcctcgat tgcattttat gagattactg
    14041 aaattaaatc tgcgcccgtg caagccgcca acaaagcaaa caatacctca caaaaagcgc
    14101 agcaaaaatg ggatggttac gcaccctatg ccttatacca aaccttagaa aatttataaa
    14161 caatacaatc attaaagtaa taattttagt caaattcttg ccacaacctt tttagctgcc
    14221 ctgcaagcct tataaataaa ggctcacagg gcaaatattt tttctcgcac aatttaatgc
    14281 ataaaaatat gattcatatt gtgcaatcta gggttaagat taaaccgcaa gattaaagcg
    14341 taaaattaaa gcgtaagcgt agcaagttaa aaatttttaa tttaattagg agttaagttg
    14401 aaatgaaacc agaaaccatt gccattcaca caggttacag cccagaaccc accactaaag
    14461 cagtggctgt gcccatttat caaaccactt cttatgcgtt tgataatacg caacacggag
    14521 cggatttatt tgacctcaaa gtgcaaggca atatttacac gcgcattatg aaccccacca
    14581 ccgcggtgct tgagcaaaga ctagccgcct tagagggcgg tatcggtgcg ctggctgtgg
    14641 cttctggcat ggcggccatc acttatgcga ttcaaaccat tgctgaggct ggcgataata
    14701 ttgtgtcagt ttcaacgctt tatggcggta cttataattt atttgcccat accttaccca
    14761 aacaaggcat tgaagtacgc ttttttgatt acaacgaccc aaccgcgcta gaaaaactca
    14821 ttgatagcaa aaccaaactc gtgtttgccg aatcgattgg caatccgctt ggtaatattg
    14881 ttgatattgc cgccattagc aaaatagcgc acaagcatgg cgtgccagtg gtggtcgata
    14941 acaccgttgc cacgcccatt ttgcaccgcc cttttgaaca tggcgcagat attgtggtgc
    15001 attcactcac caaatatatt ggcgggcatg gcaactcgat tggtggcatt attattgata
    15061 gcggtaaatt tccgtggggc gaacacaaag cacgttttcc cgttctcaat acgcctgacc
    15121 ccagctatca cggtgtcaat tatgttgagg ccttaggtcc tgctgcttat attgcccgtg
    15181 cgcgcgtagt gccgctacgc aatactggcg cggcactcag cccatttaat acgtttttga
    15241 ttttgcaagg ccttgaaacg ctcgccttgc gcatagagcg ccatagcgac aacgcactgc
    15301 gcgtggcaca atatttaaaa cagcataaaa aagtgaaatg ggtgaaatac gcaggattaa
    15361 gtgatcaccc agagcacgca ctggtacaca aatatttgag tggcaaagca tcaggtattt
    15421 taacgtttgg tgtagcgcat ggcatagcag gcggcacacg ttttattgat gcccttaaac
    15481 ttatcacacg cttggtgaat attggggatg ccaaaagctt agcctgtcac cctgccacta
    15541 ccacgcaccg tcaattaaat gcagaagaac tcaagaaagc tggagtgagt gaggatatgg
    15601 tccgtatttc tgtaggcatt gaaaatgtag aagatattat tgaagatcta gagcaggcgc
    15661 tatcacaagt ctaattcaat gcgtgtcgtt gtaaaactgt aggaaaaagt gcttgtggct
    15721 taacacttaa ggaagcgtcg ttatctccct aacctcattc cggcatcagt aaaagtcact
    15781 gatgccggat tttttgcaaa attgtgatgc tttactatag tccgccaggt gctttatgtg
    15841 aggtcaggcg caataatgca ctcaccatca catctacttc tgccgtggta ttataaaacg
    15901 ctaacgatgg ccgcacggtc gtttcaaccc caaaacgccg caatatcggc tgcgcgcaat
    15961 ggtggccaga acgtaccgcg ataccctctt tatttaaggc cgcacccact tcttcactct
    16021 catagccatc aagtttgaaa gacagcacgc tggctttttc ttttgccgtg ccaatcaagt
    16081 gcaagcccgg aactttgcac atggctgctg tggcgtattc cagcaactca tgctcgtagc
    16141 gggcgatgtt atcgatgcca atccgttcca catattctaa agccgcacct ataccaactg
    16201 catccgcaat attgcctgtg cctgcttcaa acttcgcggg cgcttcatga tagacggttt
    16261 tatcaaacgt tacatcttga atcatattgc caccaccttg ccaagctggc atgtcgtcta
    16321 acacctcttt tttgccgtaa agcgcgccaa tgcctgtggg gccaaacact ttatgtccag
    16381 agaacacaaa aaaatcaggg tctagcgcct gcacatctgt acgcatgtgc gacacagact
    16441 gcgcgccatc caacagcacc ttagcgcctg cggcatgcgc catttgtatc atctgcgcgg
    16501 caggtgtaac cgtacccaag gcgttagaca cttgcgtaaa tgatactagc ttgacgcgtg
    16561 aagtgagcag tttttgatat tccgctaaca acacctgacc gctatcgtcc actggcgcca
    16621 ctttcagctt ggcacctgtt tcttggcata gctgctgcca aggtacaata ttggcgtgat
    16681 gctcaaggtg tgaaatcaca atttcatcgc cctcaccaat atgctttttg ccccaagttt
    16741 tggcaactag gttaatcgct tcagtcgtcc cgcgcacaaa cacaatatta tctactgatg
    16801 gtgcgttaat aaaacggcgc accgtttcac gcgcaccttc ataggcatcg gtggcacgcg
    16861 cggctaactc atgtgcggca cggtgaatgt tagagttttc atgtgcataa aaatatgaaa
    16921 tccgatcaat caccgcctgc ggtttttgcg tggtggcggc attgtcaaac caaatcagcg
    16981 ggcgtccatt caccaactca cgcaaaatag gaaaatcttt gcgtactaaa ttggcatcaa
    17041 atggtggatg cgcactggcg actggccctg caaccgcagc gtcttgctgg tgaggtgagt
    17101 cctgtaaaaa ataatagggc gcagcgcctg cttggcttgt agtttggctg aaagcttggc
    17161 tagacttttg gttagttcct gcgctactaa tgccagattg gctgtctaat ccactcaaca
    17221 gttgattcaa ctccgcctga taagggtcat caataccgcc aagcaactgc gcaatatcaa
    17281 acaccccgct aatgggcaat tgattggtgt agccagtatt agtcacatct gtgggcgata
    17341 aatttggtac gccagaagcc acacttggca ccgctgaaaa tatcgaaggc gttggtgttt
    17401 gcaccgggtc gctaatgata ttgggactgg gcagcaccgc attggccgcc acagggttga
    17461 gcgatgcatt cgatggaaaa tcctcttttt gtgcaaacgg cgaacctgct ggaaaacctt
    17521 ttgcataaag ttcattggca atgcggctca gttctgcaac atcaaagtgg ccagtttcac
    17581 tggcctgcct gccaatagcc aaagcagcag ccatgcgtgg atgctcaccc ggcagcgcgg
    17641 ctaaaatcgc ctctggttca aaggaggata aatcacctaa tgcaggtgaa aatccattat
    17701 ttgtagtcat aataattgcc tacatccaca ttttctaata cggcaatcgc gtcatccgtc
    17761 aatacagcca atgagcaata aagcgatact aagtaagaag caatggcttt gtggttgatg
    17821 cccataaagc gcacggacaa gcccatgctt tgttgaccag gtaggttagg ttgataaaga
    17881 ccaacaacgc cttgacggct ttcacccgtg cgaagcaaga ggatatttgt tttgcctttg
    17941 gtcacgcgca atttatcaca agggataatc ggcacaccgc gccatgtaat gaactgtgag
    18001 ccaaacaaag ccacggttgg aggtggcaca ccacggcgtg tacactcacg gccaaaggca
    18061 gcaatggcct gcggatgtgc taagaaaaaa gctggttctt tccatacttt agtcaataat
    18121 tcgtcaaaat catcaggtgt tggtgcgcct gtgcgtgatt tcactttttg tgaatctgca
    18181 atattattta ataggccata ttctttattg ttgatgagtt cgctctcttg gcgctctttc
    18241 acggtttcaa tcgttaagcg caattgctct ttaatttggt tgtgagggct gctgtataaa
    18301 tctgacacgc gagtatgcac atctaccgcc gtgttgactg catttaacac atactcacgg
    18361 ccccactctt cgtaatctac aaaagtttgt ggaatttcac gctcatcttt tcctgaacag
    18421 tctacttcaa tcgcgctagg gtcttttacc ttattcacac ggtagatacc cgcttcaacg
    18481 gggacccatt gtaataaatg tactaaccaa cgtggggtaa tcgttcccag cattgggacg
    18541 gttttggttg cgttcgatag cgtgcgcgcg gcaacgtcac ctagtgcggt atgggcttga
    18601 tgaatatcag tcatgagttt ctccagtgat aaatttaaaa aatgttgtta ttttgtattt
    18661 tctacggcta cagtttcttg aatacttgct tgtgattgcg cttgggtgat ttggctatcg
    18721 ggtggtacgc tgcgcgtcag ccacacatta ccgcctatca ctgaacgtgc accaattgtg
    18781 atgcgcccca gtactgtggc accggcataa atgaccacgt catcttctaa aatagggtgt
    18841 cttgcactgc ccttaatgag gtgtccatgt tcatcagtag gaaagcgctt tgcgcctagc
    18901 gtgactgcct gataaagcct gacatgattg ccaatcaccg ctgtttcgcc aatcacaacg
    18961 cctgtgccgt gatcaataaa aaagccctca ccgattgtgg cgccggggtg aatatcaatg
    19021 ccagtcgccg agtgtgcgag ttctgaaata atacgggcaa tgagtggtaa accaagtgtg
    19081 tgcaactcgt gcgcgagtcg ataatgaata atggccgtga tacccgggta gcaaaccaac
    19141 acttcatcca aactccgtgc agccgggtca ccctcaaagg ctgcatttaa atcggtgtca
    19201 atcagttgac gaattttggg tagtgttttt gcaaaagcgc gggctttatt aagcgcagct
    19261 tctacactgg gcgttttgtc gtggccagta tctgcagagt aacgcaactc taattgaatt
    19321 tgggtgttga gctctcttaa tgcggtatct agcgtaaagc ctacataata atcaatgcct
    19381 tcgtctttga ggccaggggc acctaaacgg tttggaaaaa gtgccgcact taaaccatcc
    19441 acaacgcttt gcagtgcttt tcttgaaggc aattttggtg gtttattttt tcggtgtcgg
    19501 tgctcaagtg aggttagcct aacatctcgc aaggcctgca caatgcctga aatatcaaag
    19561 tcttcattcg ttagcgttgc tgtttgattg gtcatcatgc atacctcgag cagaaaataa
    19621 aaaagccagt atgtttttca acataccggc ctccagtgtt ctggtcagca cacagctttg
    19681 taaatattat acctaagcaa attctttaat agataataat ttttagttat aagcatatct
    19741 tattatattc ttttacttaa acgccagtcc cttatatatt ggctgcctta ttacagttaa
    19801 aggtaagtat tcaatctatt atgcatattc aaactattat tcatcaacta aaacttactg
    19861 cagtggcgct tgcaggtgca ttcgtttttt caaatagtgc tagcgctgaa acctcgttgt
    19921 taaatgtttc atacgatgta tcgcgtgagt attacaaaga cctcaaccct gcgttcatac
    19981 aatactggaa agaaaaaaca ggggaaacca tcactatcaa tcaatcacat ggtggttcta
    20041 gtaaacaggc tcgctctgta gtcgatggcc tgtctgcaga tgtgattacc atgaaccgct
    20101 cacctgatat tgatatttta tatgagcgcg caaaactcac tcctaaagat tggcaaaagc
    20161 gtttaccgca tgctagctct ccaaccgctt caacttcagt attcttagtg cgcaaaggca
    20221 accctaaaaa tattaaagac tgggatgatt tagtcaaacc aaaagttgct gttgtgattc
    20281 ctaacccaaa aacttctggt aacggtaaat atacttattt agcggcatgg ggctacgtgc
    20341 ttaaaaatgg tggcgatgaa gctaaagcca aagagtttgt gaccaaactc tttaaaaatg
    20401 tgcccgtgtt agacacaggc gggcgtggcg caacgactac atttgcacaa cgtgatatcg
    20461 gtgatgtgtt actcacattt gaaaatgaag tatttcttat acaaaaagag ttgggcgctg
    20521 ataaatttga agtcatctac ccttctatta gtgtgttagc agaaaaccca gttgcattgg
    20581 tggataaagt ggtcgataaa cgcaaaactc gcacgcaagc acaggcttat ttagaatttc
    20641 atttttctga agttgggcaa gatattgccg ccaaacatta cctacgacca caagtagaca
    20701 gcgctgttac caaacatgct gcaaccttta aaccacttaa attatttaag gttgaagaag
    20761 tatttggcag ttggagcaaa gctgaaaaaa cgcactttgc cgatggcggc gtgtttgacc
    20821 aaatttatgc taagtaagca acgtaaatga cttggtaaac aatttggcaa aatcattgga
    20881 agaaaattat gactaaacac cagcaacagc aagcgagctt ggctttactt aaagagcaat
    20941 taaaggagtc actacaactt gacttagatg actttgcgtt agaggccaat gcgttgcatg
    21001 aatatgtcag caaatttgtc ggcatcaatt taaagtcatc atttcagcca atttatgaca
    21061 ttgaaaaagg cgatttgcac ggctttgagg cctcacttct agcaagtttg ggtgacattc
    21121 aagaaacgac cgctgatttt gcttattcct acgctaaggc ctcaggcaag ctcgttaagt
    21181 ttgaccgcgt agtccgcacg ttgcacttac ttaactacaa acaaatattc caagaaaaag
    21241 gtttactgtt tttaagtgtg catcctgatt tactgattag cgtgaatgag cacggcaaag
    21301 tgtttgagcg cattttgcat cagtattcat tgcctactga acgcgtcgtg attcaaatca
    21361 gagattacag caccaccgaa caaatcgaaa atcttgtcac ctatgaaaga caattggctg
    21421 cggccattga aaactatcat gatcgaggct ataaaattgc cattgattac tttggtaata
    21481 agcattcgct ggtcagtcgt ctgtggaaga tctcacccga ctacatcaag tttgacccga
    21541 gcttgataca agaagctgaa agcaatgtgc ggttaagaac aacattgaaa aatcttactg
    21601 acttaatcaa agacttagat acaacgccaa ttttgaccaa cgtagtaacg caggcgcagt
    21661 tagatattgc cctacatgca ggcatttcgt ttgtacaagg tgattatttg ggtaaatcaa
    21721 gctcgtcatc acaactgcat gcctcagaat tgattcaaaa gaggtttgtg gcttcttaat
    21781 tttaattaac cccatcatcg cgctagacaa tgtccgattg cttgcacaac attctatata
    21841 acttttagcg ataaatttat gtcataaaag tatttaatat gcataaagca aactggtaaa
    21901 gtacgcgcaa cttcaatata ttacaaatag accaaataaa aatatggcca aaaaaagctc
    21961 actcccaggg tttaatctat cactcggtta tacgatattt tatttaagtt taatcgtact
    22021 tattccctta tctgctgtct tttttaaaac atttacccta agttgggatg aatttgttca
    22081 ggccgttgcc tcacctagag tcgtggcctc atacaaactc acgtttggcg catcgctaat
    22141 tgctgcaagc atcaatgtag tgtttggcgt actcaccgct tgggtgctga cccgatatga
    22201 attttttggc aaaaaaattg tggatgcgtt tgttgatttt ccatttgcac tgcccacagc
    22261 ggtggcaggc atttcactgt cagcaattta tgccaaaaat ggctggattg gcagcctgct
    22321 agaccaagtt ggcatcgccg tggcctatac acctttaggg gttgtggttg cactcacttt
    22381 tattggttta ccttttgtgg tgcgtaccgt gcagccagtg ttagaagact taggcgcaga
    22441 agtcgaagaa gctggggcta gcttgggggc aaaccgttgg caagtgtttt ataaaattat
    22501 cttgcccgcc atttggccgg cagtcattac agggtttgca ctcgcatttg cgcgcgcagt
    22561 gggcgaatac ggctcggtaa ttttcattgc cggtaatatg ccaatggttt ctgaaattac
    22621 gcccttgatt attattacca aattagagca gtatgattat gctggtgcta ccgccgtggc
    22681 cgcagtcatg ctagtgattt cttttatttt gttgtttgca atcaatgggt ttcagtggtg
    22741 gagcaaccgc cgcttgggcc atgtgaaata aagaaatctg gagaataata tggctgcagc
    22801 caacgcacat tcattatcac aatacgctaa gtaccataac aaggtcggtc gcagtcgcgc
    22861 gacctctgaa gccgcatggg tgcgttatac gctcattggg gtggcattgt tgtttttatg
    22921 cttattttta tttattcctt tagcggccgt ttttgttgaa gcgttcagaa aaggcgctga
    22981 aacttatttt gcttctctca ctgaagctga cgcgctttca gccatgaaac tcactttaat
    23041 agcagcggcg atttcggtac cgttaaactt agtgtttggg gtatcggccg cctgggcaat
    23101 ttcaaaattt aaatttaagg gaaaaagctt tctgattacc ttaattgatt tgccgtttgc
    23161 aatttctccc gtcattgcgg gtttaatcta tgtgttgatt tttggtttgc aaggctggtt
    23221 tggcgagtgg ctatcggatc acgattttaa aatcatcttt gccgtacctg ggattgtgtt
    23281 agccaccatc tttgtgactt ttccatttat tgcccgagag cttattcctt taatgcaggc
    23341 acaaggcaaa gatgaagaag aagctgccat ggtattgggt gcatcgggct ggcaaatgtt
    23401 ttggcacgtc actttgccca acattaaatg gggcttgctc tatggcgtga ttctttgtaa
    23461 cgcccgcgcc atgggtgaat tcggtgcggt ttcagttgta tcaggtcaca ttcgtggttt
    23521 aaccaacacc atgcctttgc atgttgaaat tttatataac gaatacaact acgcggcagc
    23581 ctttgcagtg gcatcggtat tagcactttt ggctttggta acattggcac tcaaaaccta
    23641 tgtggaatgg caagccgcaa gaaatgccga agaaactgaa cgagaaatca ccgaataaga
    23701 caatgctttt tattgtggta tcaatcaaat aaaacaagat taggaaatct ggcatgagca
    23761 tagaaatcaa aaatatcaac aaaggttttg gcagttttag cgcactcaac aatgtgtcac
    23821 ttgaagtacc cacgggcgaa ttagtcgcgt tattagggcc ttctggatgc ggcaaaacca
    23881 cattgctacg catcattgca ggcttggaaa cgccagacac cggtagcatt catttttatg
    23941 gcaaagatgc aaccgaacgc gctgtgcgtg aacgtgaagt gggctttgta tttcagcatt
    24001 atgcgttatt tcgccacatg accgtttttg aaaatgtggc ttttggtctg cgcgtgcgcc
    24061 ccaaacacac gcgcccaaat gacgccgaaa tcaaacgtcg cgtccacgaa cttttaaatc
    24121 tggtgcaact tgattggctg gcagaccgtt acccaccgca actttcaggc ggccaacggc
    24181 aacgcattgc gcttgcaagg gcgctagcgg ttgaacccaa agtgctgttg ctggatgagc
    24241 catttggcgc actcgatgcc aaagtgcgta aagaattgcg tcgctggcta cgccgtttgc
    24301 atgatgaact gcatgtcacc agcgtttttg taacgcacga tcaggaagaa gctctggaag
    24361 tggccgacac gattgtggtc atgaaccaag gtaaaattga acaaatcggc agcccgcaac
    24421 aggtatacga caacccggcc acgccctttg tgtatcagtt tttaggcaac gtcaatgctt
    24481 ttgaaggccg cgtaaatgct ggtgttgcaa aactaggggc gctcgaagtg cccgtaaaag
    24541 aattcgcaca aacccacgac agcgcagtac aagcatttgc ccgcccgcac gatattgata
    24601 tcagccgcga tgcaagcgct gcggctttta aagcacaact cacctacgta cattcagcag
    24661 gttctgttgt gcgtttggag ttaaaacgtg atgatgccga aacgtttatt gaagcagaac
    24721 tcacccgcga atactttagc caactgaatt taaaagaagg cgaaaccgtt tacttaaccc
    24781 caagaaatgt tcgctttttt cttgattaac cttgtcaaaa aatagcgaaa tcaaattcaa
    24841 taaaaccttc ttaaacacat tcataaaacg tcgagcaaaa aaatatgctc tggaagcctt
    24901 tatttacaag gcttccagag catcgaaaaa ggtttgggtt aaaattacgc gctaatactg
    24961 attttttgca cgccttaaac ttgcaaaatc tgccgttttt tcacatattc actcgccagt
    25021 tatttcacgg ctaaatttcc atttttgtaa cgcttctctt aaaatttttg ccaagcaact
    25081 tggttgacca tgacataatt ccatcctcac gattgggctt taatgaaatt tttatttaag
    25141 ttgtttggaa tactcaagtg gttaatttct ggaatataca tcacccaatc ccatgcaata
    25201 tttgattgaa tacggctata ttggcatttt tattgctgcg tttttagcgg ccacgatttt
    25261 gccattgagt tctgatgtgg ttttaagcgc gctattactt agcgaactct cgcccttcac
    25321 actgattgcc attgcaacct ttggcaatgt gcttggctca ctgaccaact acgcactggg
    25381 ctactgggca agccaaaaag tcatcaaacg atggctaaga atgtctgaca gcgactttac
    25441 caaagcacaa cagcgcttta caaaatacgg cttgctgagc ttatgttttg catgggtgcc
    25501 gattattggc gacccactca ctgtcattgc tggggtttta cgtattcgct taatttggtt
    25561 tttagtgttg gtcacaacag gtaaattact gcgctacctt gctgtcagtt actttgtgct
    25621 tttcgcctaa ttacagcaca ttgccttggc tgtcgaacaa gccttcaaac aaaatagaac
    25681 ttaaatagcg ttcacctg
//
LOCUS       DAOQSI010000012        25614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_1205_length_25614_cov_5.922258, whole genome shotgun sequence.
ACCESSION   DAOQSI010000012 DAOQSI010000000
VERSION     DAOQSI010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 25614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25614
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1205_length_25614_cov_5.922258"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="PKL42_01805"
     CDS             complement(<1..547)
                     /locus_tag="PKL42_01805"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897311.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNU65834.1"
                     /translation="MATRKDLANAIRALSMDAVQKANSGHPGAPMGMAEIAEELWNHH
                     LSHNPKNPNWSNRDRFVLSNGHGSMLIYSLLHLTGYALPMDELKTFRQLHSKCAGHPE
                     YGYAPGVETTTGPLGQGIANGVGFAMAEKLMADQFNKPGHNIVDHHTYVFLGDGCMME
                     GVSHEACALAGTWGLGKLTAFW"
     gene            complement(958..2004)
                     /locus_tag="PKL42_01810"
     CDS             complement(958..2004)
                     /locus_tag="PKL42_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU65835.1"
                     /translation="MSEAKLNWGILGAARVNERLLPAIIEAPNSRLVAIASRRAGAAL
                     ATLQKYAPHQQRDVQCYDDLESLIQDADVQAIYCPMANEEHAEWAIKAIKAGKHVLIE
                     KPMAIRLQAINAIYAAANQYQVKVMEGFMYRFHPQHQQVQDIVASGLIGDVLSVRASY
                     SFLMKASRMYRINRNMINGGGAMWDIGPYAIHSLRWCFAQNGIPAEPKSVIAHAKLNE
                     AGADIVTSGVLDFGPDAEGRARFGHFDVSFERSRKSEYEIIGSKGWVKCHAVWVFQND
                     VPVISWALEDGRYSEERFAPVNHFTLEIEHFSDCVLNDKPPRLNFADAQGNCKAIEAV
                     LQAVTSGHQVEIAN"
     gene            2143..3360
                     /locus_tag="PKL42_01815"
     CDS             2143..3360
                     /locus_tag="PKL42_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HNU65836.1"
                     /translation="MQQNISLNEIGHSKKASQKNAFFNLGFRPFFLGASIFSIVSLTL
                     WMAVYGRHVQLSLTTVTPAQWHAHEMLFGYAFAVIAGFLLTAVKNWTGIQTLHGKPLF
                     ALFTLWCFARLTMLIAPNLIAVAAALDIAFNLALAYAVAVPIIKVKQWRQLGILAKIA
                     ILTLANAAFYAQALGLLANGAHIGIIVGLYLTISLILVISRRVAPMFIERGVKEKVVL
                     KQFKWLDISIMLFLVALLFNELVTQSALVSILMGILLFLANGVRLFNWHTKGIWKVPL
                     LWSLYCGLWLINLGFLLLALSYITSAVSIIFAMHLWAIGGIGLMTLAMMTRVSLGHTG
                     RSIHEPPKLMAYAFGIIIAGALIRAVLPIFVSQYYLAWVIGAYFCWVIGFALFFIQYY
                     RILTKPRIDGAFG"
     gene            complement(3426..3992)
                     /locus_tag="PKL42_01820"
     CDS             complement(3426..3992)
                     /locus_tag="PKL42_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNU65837.1"
                     /translation="MVALNPPVCNFGQRAADFNLLGVDGKRHTLASSAGKNGLLVMFI
                     CNHCPYVKAIKTRLVDDCRELLQYGINTIAIQSNDTENYPEDSFERMREESNLYNFSF
                     PYVLDDTQEVAKAYGAVCTPDFFGFNANLELQYRGRFDAAGRRIADQNNKRDLFNAMK
                     QIAETGEGPKEQIASIGCSIKWFAEDSI"
     gene            4084..4818
                     /locus_tag="PKL42_01825"
     CDS             4084..4818
                     /locus_tag="PKL42_01825"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HNU65838.1"
                     /translation="MANLRFFTDHALTLGATVTLSESAAAHATRALRLNVNDSVIIFN
                     GDGFDYDCTLVTVNKNAAVAKVAGQRKIENESPLHITLLQGISSGDRMDFTIQKAVEL
                     GVTNIQPIATARSVVKLSVDRAQKRTAHWQNIVASACEQSGRAFVPQVGAPMSLPQWL
                     TANPQSNQTRILLNPIGATPLKSMPKPMHEIQLLIGAEGGLSQHEIDTALAQGFQSIV
                     LGPRILRTETAALTAISAMQTLWGDF"
     gene            complement(5009..8815)
                     /locus_tag="PKL42_01830"
     CDS             complement(5009..8815)
                     /locus_tag="PKL42_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HNU65839.1"
                     /translation="MKNKLKKLLVSLPTKVFVGLFAGYLIFSYFVVNPVAKKLVPWVA
                     NKQLASQASVGRVAFDPFRLKATVEQFNLADKHGAPLAGFEKLVVDLEFSGLFDWAWR
                     FNEINLLAPQGLVAISPQGKLNWADLIQKLNEDKKPPSETIPRVVIAHIVVKQGQLQY
                     MDAHRTAPYQATLSPLDFELDGFSTLPKDRGDYLIAAKFPEHGGALKWKGDMGVNPVA
                     SKGAVAVSGLKLAKILQIVKGVELPFKPSRGDIEANFSYDFALHNDQPKVSLNGLSVR
                     LNQLAGTLSHVGEVTLSKAQFSAPKIDFSYQNQPDLIVQTLDLSLDELSIQRGKETVV
                     QLASTHITLPKLAFVQHNNHPQVQFEDLDVKVSNIQVSHGKDTLLSLPQFDINHIRLD
                     LEKRQANIAQVVLGKGIIQATRNQSGTLNWQSAFEANEVITESTALATDATPANTPVS
                     PFAIEIAEIGLQHWQAALQDQSFKQSLQVDVADINVNFSLKKPEGFWTLHRLQTALSN
                     ATVKSGTTPKPVASLDKLQLSKGEILLDKQQVNIASLQLSGLKTEIIKPADAPLNWQT
                     ILSANANTPQKVTQVKSKPTKNNDWSLNLKKLALENSQLHIEDKSNAVPVVLDVDKLA
                     LEARDATLDLSRAVPIKASFNIKQGGRFEAFGKVTPTPLKANLDLKLAALTLKPFSPY
                     LNQFALLKLNEGAGSVAGKLIVKDEKALAVAFNGGFSVDKLALLEEAGDAPFLAWEKL
                     ASDSLELSLSPNRVHMAALTITQPAGKFIIHEDKSMNITRVLRSQAQVNVGVPEEQNR
                     AAQTKEALEAKADTSNIETAKPVEMIGAAVAPKVEEKTLVAAIAPMPAEDTPPAAFPV
                     SIETVRIENAALEFADLSLKPQFGTQIHLLTGVINGVSTNPSAIAQVELDGKVDDYGA
                     ARIRGAVQPFKATNFTDLKLAFTNLEMNRLTPYSGKFAGRRIDSGKLSVDLEYKIKQR
                     QLSGENKFVINKLKLGEKVDSAEAANLPLDLAIAILEDSDGVIDLDLPITGSLDDPKF
                     SYGSIVWKAIRNVLGKIVTAPFRALGKLFGSGADKLEAITFEAGKAAISPPELEKLKA
                     VSEAFAKRPGLALGVVPSYDALLDARAIQEATLRRQVAEEIGLKLAEGQEAGPIDLTN
                     PKVQKAIDALHDTLTKKGMLKKLAAKFEKPKAGHFEEAQEKLTVSIEVKEADLQALAK
                     ARGEAIQKTLLDAGVAAERVRLQKAEVIKADSKTKAINTKLTLDVKSATKSAEPAVDA
                     PAVEAK"
     gene            8966..10546
                     /gene="argA"
                     /locus_tag="PKL42_01835"
     CDS             8966..10546
                     /gene="argA"
                     /locus_tag="PKL42_01835"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HNU65840.1"
                     /translation="MSLGNYIRHLRKSHRFTLQQLAQRVDADAGNLSRIERGELGISE
                     NLLRKIAAAFETTPATLYAESDTAQTTPVPAQLAYNNAVQNNSQEFVQWFRSATPYIH
                     AFGGKTFVIAFGGEIVDEKQFTTLTHDLNLLASLEVRLVLVHGARPQIEQRLKRAQLT
                     PLLHNGLRVTDDEAMEAVKEANGAVKVEIEALLSMGIANSPMAGADIRVASGNFVTAK
                     PIGVIDGVDLQHTGEVRKVNAAGIQKRLNEGELVLVSPLGYSPTGEIFNLSLEDVAVS
                     TAIALDADKLIFLMDSEGVQNLRGELLREMTAEKAKNLLRNVQNSEEPIHISDDIKYY
                     LPAAIHACEHGVARVHLISRHVDGAILQELFTMEGIGTMVTELPLETMRQATIDDVGG
                     ILKLIEPLENEGILVRRGRERIEMEINHFYVMEHDNRIIGCAALYPFIEERAAEFACL
                     AIDQAYRGGGRGDKLFDYCAMQAKALGFKTLFCLTTRTEHWFLERGFTEQSVDKLPVE
                     KQQLYNFQRKSKVFSKTL"
     gene            10639..11523
                     /gene="argB"
                     /locus_tag="PKL42_01840"
     CDS             10639..11523
                     /gene="argB"
                     /locus_tag="PKL42_01840"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNU65841.1"
                     /translation="MLTQTTAAKKARTLAEALPYIKRFFDKTIVIKYGGNAMTDEHLK
                     QCFAQDVVLLKLVGMNPVVVHGGGPQINEMLDKLGKKGEFIQGMRVTDEETMDVVEMV
                     LGGQVNKEIVNLINRHGGKAVGLTGQDGNFIHAHKLMMEDLKDPTKMIDVGQVGEISA
                     IDPSIINFLDSGDFIPVVAPIGVGKDGETYNINADVVAGKLAEILNAEKLILLTNTPG
                     VLDKNGELLTGLTPNQIDQLVADGTLSGGMLPKISSALDAARSGVKAVHIIDGRVEHA
                     LLLEVLTDEGVGTLIKAK"
     gene            11609..12168
                     /locus_tag="PKL42_01845"
                     /pseudo
     CDS             11609..12168
                     /locus_tag="PKL42_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184211.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
     gene            12212..12772
                     /gene="slmA"
                     /locus_tag="PKL42_01850"
     CDS             12212..12772
                     /gene="slmA"
                     /locus_tag="PKL42_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HNU65842.1"
                     /translation="MAGERKQQILETLAKMLEAPKREKITTAALAAKLGVSEAALYRH
                     FANKAQMFEGLISFIEETIFGLINKINTEETDGYRQIQRITTMLLAFGEKNPGMTRVL
                     IGDALVNEDEKLQLRINQLYDRIEVTLKQSLKISVATSGKKVMPEAQANLLICYVIGR
                     WQQFAKSGFKRKPLEFASVQVSYLTN"
     gene            12791..13012
                     /locus_tag="PKL42_01855"
     CDS             12791..13012
                     /locus_tag="PKL42_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HNU65843.1"
                     /translation="MQGTIFGLTETQITELGMSYAVPGLMLLMLFIVGHLAWQSKAGK
                     FGTMILFLALALGMVGYIAKSLIQYNLGI"
     gene            complement(13164..13466)
                     /locus_tag="PKL42_01860"
     CDS             complement(13164..13466)
                     /locus_tag="PKL42_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65844.1"
                     /translation="MLNKLKVMLFGGKPDHERRRTPRAGKVPAVGAHIGRQLLVMQIT
                     GPITRELWDWFVLMGWREVNMKTNRRQVKMLPKITFSSIAKAEISERETIYREAIK"
     gene            complement(13531..14748)
                     /locus_tag="PKL42_01865"
     CDS             complement(13531..14748)
                     /locus_tag="PKL42_01865"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HNU65845.1"
                     /translation="MQIYLVGGAVRDELLGSAVTDKDYVVVGSSVDEMLAAGFRPVGK
                     DFPVFLHPKTQDEYALARTERKVASGYKGFTIHAATDVTLEEDLGRRDLTINAMAKSS
                     DGQLIDPYGGLADIHSKTLRHVSTAFAEDPVRILRIARFAARYADFSVAPETHALMQQ
                     MVEAGEVDALVAERVWQELSKGLMEVKPSRMFEVLRSCGALKRLLPELERLWGVPQPE
                     KYHPEIDTGVHVMMVIDYAASKNFSLPVRFAALMHDLGKGTTPKEVLPRHIGHEARSV
                     SLLKDITKRLRIPTACKELAQMVAKYHGKLHAVEEMRPSTLLIFLIELDAIRQPERLL
                     HFLQACEADSRGRTGFEEREQPEALRLLNALDAALSIDAGAVASGIESPEKIKVAVFE
                     ARLAAIQSKLKFL"
     gene            complement(14773..15732)
                     /locus_tag="PKL42_01870"
     CDS             complement(14773..15732)
                     /locus_tag="PKL42_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HNU65846.1"
                     /translation="MQSIAQIKEVCVLGGSGFVGSSIVTRLDAAGFQVKVLTRRRESA
                     KHLLLLPNVRVVECNVLDYKALNRELRGADAVINLLGILHQSRRNTFNLIHQQLPAQL
                     AKICIDLNIKRLLHMSSLRADKDAPSHYLRSKAAGEAALLAEKIRLNVTIFRPSVIFG
                     RHDRFINLFASMIKFLPVILLAKPNAKFQPIWVEDVSACFLTSLENSTTYGQTYELAG
                     PKVYTFRALIQEVMIVLQIQRPIIGLNDRLSYMQAFFMEWLPIKLMSRDNVRSMEVDS
                     VCDMPFPAVFSRTPSALETVIPQYLVNQTSRGNYDRYRRHAAR"
     gene            complement(15770..17746)
                     /locus_tag="PKL42_01875"
     CDS             complement(15770..17746)
                     /locus_tag="PKL42_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNU65847.1"
                     /translation="MNNIRLFIVLVTITFSSQTFALSDEALFQRARASYAAKNDFALA
                     EEASQLKDQQYILAPYADYWLMLLKLQQNTVRDEEVQNFLAQYIDMPFTDRVRGEWLK
                     KLGKEQNWAPFFDEYTLFKREDIAVLCYALQGHQMLGNADVTAEAKMLWLTPSELPSQ
                     CNPVFDSLQVSGALTNEDIWARFRLGLHAGKLGVAKSVATRLSSVDVAHLKLLDRAYQ
                     SPQAFLDKPPVSFKSRFGVEANLYAVDRLARNNIQNAIAAYKKVQNSIDSEHRAFGWG
                     RIAYHAARSHHPQAVSYYYQAEGASLDEEQLAWKVRAALRDQDWQTVLDAVNAMQPKQ
                     LEQGAWRYWKARAIKVVEANNPEKNAEAGELLSKLSTERHFYGWLALDDMGSVLSNPS
                     VDFVPTNDEVNAIANLAGIKRALELQRLDMRMEAKAEWAWAIRGFADTELLAAAEYAQ
                     RKKWYDVAISTADNTKVTHNFNLRYPTPYREMFRTSSREVDIDEAWAYGITRQESRFM
                     HYAKSGVGASGLMQLMPATAKWVAQRKGLRGYNNSMIHDLTTNIDFGTYYMRYALDLM
                     NGNAVMATAGYNAGPSRAKKWLAAKPLEAAIYIESIPFLETRQYVQKVMANAQMYAPR
                     LLEKNPGVKIQTLKSRLGKVPGSGQTVVYDEESE"
     gene            complement(17974..18906)
                     /gene="lpxC"
                     /locus_tag="PKL42_01880"
     CDS             complement(17974..18906)
                     /gene="lpxC"
                     /locus_tag="PKL42_01880"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNU65848.1"
                     /translation="MLKQRTLKKAISATGVGLHNGEKVTLTLRPAAANTGIVFKRVDL
                     PQPNEIVASAQAVHDTRLCSALEANGARVATVEHLMSALAGLGIDNVYIDVDAAEIPI
                     MDGSAGPFVYLLQEAGVAELPVPKKFIRIKKTVEVKEQDKWARFEPYHGFKIDFTIAF
                     NHPVFEHSGCQVKIDFAKDSYIQQISRARTFGFMHEVEYLRSNGLARGGSLDNAVVLD
                     EYRVINTDGLRYDDEFAKHKVLDAIGDLYMLGHPILGAFYAYKSGHALNNQLIRALMQ
                     DETAWEYATFENKEDAPSAQQVEDVEKMHNPAFA"
     gene            complement(19094..20281)
                     /gene="ftsZ"
                     /locus_tag="PKL42_01885"
     CDS             complement(19094..20281)
                     /gene="ftsZ"
                     /locus_tag="PKL42_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNU65849.1"
                     /translation="MFEIMEKNSQEAVIKVIGVGGCGGNAVAHMIEKNVSGVEFICAN
                     TDMQALKKSQAKTILQMGMSMTKGLGAGARPEVGREAAIEDRNAIAELIDGADMLFIT
                     AGMGGGTGTGAAPVIAQVAKEMGILTVAVVTKPFAFEGKRTKVAVDGLEELSKYVDSL
                     IVIPNEKLMEVLGEDVPFLEAFRAANDVLHNAVSGIAEIINCPGLVNVDFADVRTVMS
                     EMGMAMMGSAVATGPDRARIAAEQAVASPLLEDVNLANARGVLVNITTSASFKMKEYY
                     DVMNTIKAFTAEDATVIVGNVFDESMGDGLRVTMVATGLYGASRRQQKPELRVMTQEV
                     VRNGTTNQPMYMGADVGAASVMTDDAPQVFGSNSRRAQAEAMKMAGVEEYDIPAFLRK
                     QAD"
     gene            complement(20379..21617)
                     /gene="ftsA"
                     /locus_tag="PKL42_01890"
     CDS             complement(20379..21617)
                     /gene="ftsA"
                     /locus_tag="PKL42_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNU65850.1"
                     /translation="MSRVKEDKNLIVGLDIGTSKIVVIVAELLPEGAVKVIGLGQHIS
                     RGLKKGVVVNIESTMQSIQRALEEAELMADCKINNVFTGIAGSHIKSLNSHGMVKIKD
                     AEVSQMDVDRVVETARAIALPADQQILHILTQEFIIDGQEDVREPLGMSGMKLEVKVH
                     IVTGAVAAAQNIVKCIKRCGIEVTDLILQPLASSLAVLTEDEKELGVCLVDIGGGTTD
                     IAVFKQGAIRHTAVVPIAGDQMTNDIAVAFRTPTQSAEDIKIKHGCALRQLADPREVV
                     EVPGVDGRDARQLSVQTLAEVLEPRIVELYELVLSELRRSGMEDMIASGIVITGGSAM
                     MRGMVELGEEIFHMPVRMGLPRYVGGLSEVVSNPRYATGVGLVLMGKQQLERHLTGQL
                     ESTSFGRMLEKMKSWFQGNF"
     gene            complement(21647..22447)
                     /locus_tag="PKL42_01895"
     CDS             complement(21647..22447)
                     /locus_tag="PKL42_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HNU65851.1"
                     /translation="MWDKPKLLNWIANLLFALSMVAILYAVLYAVVHLPIFPLREVKI
                     DGQLNHVSREQVKLIVSKHLKGNFFTLDLMKARTAFEKLPWARNVSIRRRWPDKLEVV
                     VEEHQELARWGSVALVNTHGELFHAASNSELPVFNGPADSVIEVASQFGNFSKALQEA
                     NLKVTSLTLTPRRAWEVTTSSGMHIELGRVEMQNRIEKFANVYVSTIAGLNKKIAYAD
                     LRYPNGFAVRRPAADLKQMEEKAKEVNANKKKPVVQVKPKPTDISQQI"
     gene            complement(22437..23360)
                     /locus_tag="PKL42_01900"
     CDS             complement(22437..23360)
                     /locus_tag="PKL42_01900"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNU65852.1"
                     /translation="MRKDFGKVAVLLGGKSGEREVSLKSGAAVLAALKGKGIDAHAFD
                     PRDKPLHDLESFDRVFICLHGRFGEDGCMQGALEMLHIPYTGSGVMASSIGMDKWRTK
                     LLWRAMNVVTPDFELVTANSDFAAIETKLGLPLFVKPANEGSSIGISKVKTAGGLQAA
                     YELAAKADPLVIAEKFVGGGEYTVGIIGDATGGYTALPIIRIVPKNEFYDFEAKYLRD
                     DTEYLCPSGLSEEKEKQIQKEALIAFKTIGCRGWGRVDFLMDASGKHYFLEVNTSPGM
                     TDHSLVPMAAKAAGISFEDLVVRILELADVG"
     gene            complement(23353..24204)
                     /gene="murB"
                     /locus_tag="PKL42_01905"
     CDS             complement(23353..24204)
                     /gene="murB"
                     /locus_tag="PKL42_01905"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNU65853.1"
                     /translation="MNEPMARYTSWRVGGIADKAYVPAGLEDVAAFLQSLDKDEPVYF
                     VGLGSNLLVRDGGVRGTVILMHNALADMGMVDNKLYAESGVTCSKVAKFSAKQHRHGA
                     EFLAGIPGTIGGALAMNAGCYGAETWDVVNKVMTVDRQGVIRSRNKAEYRVSYRHVEM
                     PAEEWFLAAWFDLPEGDMNEAEQKIKALLAKRLATQPLNLPNAGSTFRNPEGDYAARL
                     IEASGLKGYVMGGAQVSEKHANFIVNLGTATALDIELLIRHMQETVMDKFGIALQQEV
                     RVIGEYA"
     gene            complement(24265..>25614)
                     /gene="murC"
                     /locus_tag="PKL42_01910"
     CDS             complement(24265..>25614)
                     /gene="murC"
                     /locus_tag="PKL42_01910"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNU65854.1"
                     /translation="MSGIAEVLLNLGFSVSGSDLAVNATTKRLVDFGATVYQGHVSEN
                     LGDADVVVVSSAVNEANPEIKAARAKSIPVVPRALMLAELMRFRQGIAVAGTHGKTTT
                     TSLIASILAEAGMDPTFVIGGKLEAANANAKLGTGEYIVAEADESDASFLHLTPVMAV
                     VTNIDQDHMDTYEHNFDKLKSAFVEFLQQLPFWGMAVVCVDDANIREILPRVTKPVMT
                     YGFSEEAKVRAINVRADDGKMHFSVQRINGVITEFDVTLNLPGNHYVLNALAAIAIAS
                     ELNVPDEAIIKALKEFKGVGRRFERYGEISVSASSGKGTFTLIDDYGHHPVEMQAVIA
                     AARAAFPNRRLVMAFQPHRYTRTRDCFEDFVRVLSSADVVLLTEVYAAGEQPIIAADT
                     RSLIRAIRVAGKVEPIFVETTDALPEAILSIAQPNDVVIVMGAGSIGQVASRTRGMA"
BASE COUNT         6870 a         6388 c         5119 g         7237 t
ORIGIN      
        1 cccagaatgc agttaactta cctaaacccc atgtgcccgc taaagcacaa gcttcgtgag
       61 aaacgccttc catcatacag ccgtcgccca agaacacgta tgtgtgatgg tcaacaatgt
      121 tatggcctgg tttgttgaat tggtcagcca ttaatttttc agccattgca aaaccaacac
      181 cgtttgcaat gccttggcct agtggacctg tagttgtttc aacgcctggc gcataaccgt
      241 actctgggtg gccagcacat tttgaatgta attgacggaa tgtttttaat tcatccattg
      301 gcaacgcgta acctgtaagg tgcaataatg aataaatcaa cattgaaccg tgaccattag
      361 aaagcacaaa acgatcgcga tttgaccagt ttggattttt tgggttatgg cttaaatggt
      421 gattccaaag ctcttcagca atttccgcca tgcccatagg cgcgcctggg tggccagagt
      481 tagctttttg taccgcatcc atcgataacg cgcgaatagc gttggctagg tctttacgtg
      541 ttgccatagt tttctcccta atgattaact tgaagtctta ttatgaagtg caataaaact
      601 ttgattgaat tctttaaaaa gccagtcaaa ttaccgcttt taatgaaaga ctaaattatt
      661 gcttaaatag cctatttcag caagggctta gggggctatt gacgcgcaaa taatttacgt
      721 aagatttgcc aaaaaattat accaatgcga aatgttcgct ttgtaatcgt agtggtagcc
      781 aataaaaaat cacttctttt tctcacctaa atttattcaa ttttagcttg atttacctca
      841 caaccttttt cgatgccctg cgagccttat aaataaaggc tcgcagggca ttattttttc
      901 tcgaggtttt tttgatgcgt ttgactgttt ttttatagca ctaacattag gcgcttttta
      961 attggcaatt tccacttgat ggccgcttgt aacggcctgt aaaaccgcct caatggcttt
     1021 gcaatttcct tgagcgtcgg caaaatttaa gcgtggcggc ttatcgttga gtacgcaatc
     1081 actaaaatgc tcaatttcta aggtgaagtg gttgacgggc gcaaaacgtt cttctgaata
     1141 ccggccatct tctagcgccc agctaatcac cggcacatcg ttttgaaaca cccaaaccgc
     1201 gtgacatttc acccagcctt tgctgccgat gatttcatat tctgatttgc ggctacgctc
     1261 aaagctcaca tcaaaatggc caaaacgtgc gcggccttct gcatctgggc caaagtccag
     1321 cacaccgctg gtgacaatat ctgcgccagc ttcattaagc ttggcgtgcg caataacaga
     1381 tttaggctcc gcaggaatgc cgttttgcgc aaaacaccag cgcaaactat gaatcgcgta
     1441 gggtccaata tcccacatgg caccgccacc gttaatcatg ttgcgattga tgcgatacat
     1501 gcgcgacgct ttcattaaaa atgaataact cgcacgcacc gacaacacgt cgccaatcaa
     1561 gccagacgca acaatatctt gcacttgctg gtgctgcgga tgaaagcggt acataaaacc
     1621 ttccatcacc ttgacctgat attgatttgc cgccgcataa atcgcgttaa ttgcctgcaa
     1681 gcgaatcgcc atcggctttt ctatcaacac atgtttgcct gctttaatgg ctttaatcgc
     1741 ccattccgcg tgttcttcat tggccattgg gcaataaatc gcctgaacat ccgcatcttg
     1801 aatcaaactt tctaaatcat cataacattg cacatcgcgc tgttgatgtg gcgcatattt
     1861 ttgcaacgtt gccaatgccg cgcccgctcg acggctagca atcgccacca gacgtgagtt
     1921 cggtgcttca ataatcgccg gcagtaaacg ctcgttcacg cgtgcggcgc caaggattcc
     1981 ccagttcagt tttgcttcgc tcatgttcac caccattttt taatgtacat atgcagatat
     2041 tatactgatt tcatccgtcg ttgctgcatg cattgcactc acgcggcgac aagcttatga
     2101 ttggcatctt tgggtaaaaa cccaaattcc cgaggcactg ccatgcaaca aaatatttcg
     2161 ctcaatgaaa ttggtcactc aaaaaaggcg tctcaaaaaa atgcattttt taatttagga
     2221 ttcaggccgt ttttcttagg tgccagtatt ttttcgattg tgtcgctgac attatggatg
     2281 gcggtgtatg gcaggcacgt acagcttagc ttaacaacgg tgacgcccgc gcaatggcat
     2341 gcgcacgaaa tgctctttgg ttacgctttt gctgtgattg cagggttttt actcaccgcc
     2401 gttaaaaact ggactggcat acaaacatta cacggtaaac cgctatttgc gcttttcact
     2461 ttatggtgtt ttgcgcgatt aaccatgctc atcgcaccca atcttattgc cgttgcggca
     2521 gcgttagaca ttgcatttaa cctagcactg gcttatgccg ttgccgtgcc catcatcaaa
     2581 gtaaaacaat ggcgtcaact aggcatttta gccaaaattg ccatactaac cttagccaat
     2641 gcggcctttt atgcccaagc gctgggtctt ttagccaatg gtgcgcatat cggcattatc
     2701 gttggtctgt atttaaccat cagcttgatt ttagtcatca gccgcagagt ggcgcccatg
     2761 tttattgagc gtggtgtcaa agaaaaagtg gtactcaagc aatttaaatg gctagacatc
     2821 agcatcatgc tctttttagt agcattatta tttaacgagc ttgttacaca aagcgcccta
     2881 gttagcattt taatgggcat tttattattt ttagccaacg gtgtcaggct gtttaattgg
     2941 cacacaaagg gcatttggaa agtgccgctg ctatggagct tgtattgtgg actctggctc
     3001 atcaacttag gctttctgtt gttagcactt agctatatta ccagtgcggt tagcataata
     3061 tttgccatgc atttgtgggc aattggtggc attggtttaa tgacgttggc gatgatgacg
     3121 cgagtctcat taggccacac gggcagaagc attcatgaac cgccaaaatt gatggcttac
     3181 gcattcggaa tcatcatcgc tggggcgctg atacgcgcag ttttaccgat tttcgtgagc
     3241 caatattatc tcgcttgggt catcggcgct tatttttgtt gggtgatagg attcgcgcta
     3301 tttttcattc aatattacag aatactgaca aaaccgcgca tagatggcgc atttggctaa
     3361 taatttacat tgcacgaaca ccaataggcg cccaacgcac aagcatcggg cgcacttttt
     3421 ttaaatcaaa tcgaatcttc tgcaaaccat ttaatactgc aaccaatgct ggcaatttgc
     3481 tcttttgggc cctcgccagt ctcagcgatt tgcttcatcg cattaaacaa atcacgttta
     3541 ttgttttgat cagcaattct gcggcctgca gcatcaaaac ggccacggta ttgcagctct
     3601 aaattggcat taaacccaaa aaaatctggc gtacagactg caccataagc tttggcgact
     3661 tcttgcgtat catccagcac atacggaaac gaaaaattgt agagatttga ttcttcgcgc
     3721 atgcgttcaa aagaatcttc cggatagttt tctgtatcat tactttgaat cgcaatggtg
     3781 ttaatgccat attgcaataa ctcgcgacag tcatccacca aacgcgtttt aatggcttta
     3841 acataagggc aatgattgca gataaacatc accaacaaac cgtttttacc cgcactgcta
     3901 gccagggtat gacgtttacc atcaacaccc aacaagttaa aatccgctgc gcgttgaccg
     3961 aaattacaga caggtggatt gagagcgacc atgttattct cctcgatttc gttataatta
     4021 ttgatgcgct gatactagcg tttatattat cactttttaa ccctttgtta agcggaagac
     4081 ttcatggcaa atttgcgctt ttttactgac cacgcactca ccttgggcgc caccgtcacg
     4141 ctttcagaaa gtgcggctgc gcatgcaact cgcgcactca gattaaatgt gaatgattca
     4201 gtgattatct ttaatgggga tggttttgat tacgattgca cgttggttac ggtcaacaaa
     4261 aatgccgccg ttgccaaagt ggccggacaa cgaaaaattg aaaacgaatc accactgcac
     4321 atcacgcttt tgcaaggcat ctcaagcggc gaccgcatgg atttcaccat ccaaaaagcc
     4381 gttgaattag gtgtaactaa catacagccc attgccacag cacgtagcgt ggtaaaactc
     4441 agtgttgatc gtgcacaaaa acgtaccgca cattggcaaa acattgttgc gtctgcctgc
     4501 gagcaatctg gccgcgcgtt tgtgccgcaa gttggcgcgc caatgagcct gccgcagtgg
     4561 cttaccgcca atccgcaatc taatcaaacg cgtattttac ttaacccaat aggtgccaca
     4621 ccgctcaaaa gcatgccgaa accaatgcat gaaattcaat tgctgattgg tgctgaaggc
     4681 ggactaagcc aacacgaaat tgacacagcg cttgcacaag ggtttcaatc gattgtgctt
     4741 ggcccgcgta ttttgcgcac agaaaccgca gcccttacag ccatttccgc catgcaaacg
     4801 ctttggggtg acttttaaaa actcatccag aaatctacat taaacctgct tttaacatgc
     4861 gtttaaaaac cgttaatttt aagctttttt aagcacaacc tttttcgatg ctctggaagc
     4921 cttgtaaata aaggctcgca gaggcaaaaa atttcctcgt acaaaatttc atgctaaaaa
     4981 cgcgtttttg cacatctgtt tttctgtatt attttgcttc aaccgcaggc gcgtcaacag
     5041 caggttctgc cgattttgtc gcgcttttaa catccagcgt cagtttggtg ttaatcgctt
     5101 ttgttttact atcagcttta atgacttccg ctttttgaag acgcacacgt tctgccgcaa
     5161 cgcctgcatc caacaaggtt ttttgtatcg cctcgccgcg cgcttttgcc agtgcttgca
     5221 agtcggcttc tttgacttca atacttacgg tgagtttttc ttgcgcttct tcaaaatgcc
     5281 ccgctttagg tttttcaaat tttgcagcca gtttcttgag catgcccttt ttagtgagcg
     5341 tgtcgtggag tgcatcaatg gctttttgca ctttaggatt tgttaaatca atcgggcccg
     5401 cttcttgtcc ttcagccaac tttaacccaa tttcctctgc cacttggcgg cgaagcgtgg
     5461 cttcttgaat tgcgcgtgca tctagcaacg catcgtagct cggcacaaca cctaaggcca
     5521 acccaggccg cttagcaaaa gcctcactca cggccttgag tttttcaagt tcaggcgggc
     5581 taatggctgc tttgccggcc tcaaaggtaa tggcttctaa cttgtcggcg ccactgccaa
     5641 atagtttacc caatgcgcga aacggcgcgg ttacaatttt acccagcaca ttacggatgg
     5701 ctttccagac gatgctgcca tagctgaatt ttgggtcatc caaactgccc gtgataggca
     5761 agtcgagatc tatcacgcca tcgctgtctt ccaaaatcgc aatggctaaa tccagcggca
     5821 agttggcggc ttcggcgcta tctacttttt cgcccaattt cagcttgtta atgacaaact
     5881 tattttcacc cgacaattgc cgttgtttaa ttttgtattc taaatctacc gacagcttgc
     5941 cagaatcaat acgcctgccc gcaaatttac ctgaatatgg tgtgagacga ttcatttcta
     6001 agttggtaaa ggctaatttt aaatcggtga aatttgtcgc tttaaatggc tgcactgcgc
     6061 cacgaatgcg tgccgcacca taatcatcca ccttaccatc tagctccact tgcgcaattg
     6121 cactcggatt tgttgacaca ccattgatga cccccgtgag caaatgaatt tgggtgccaa
     6181 attgcggctt gagcgaaagg tcagcaaatt ctagcgccgc attttcaata cgcacggttt
     6241 caatgcttac aggaaatgcc gctggcggcg tatcttcagc tggcattggc gcaattgcgg
     6301 ctactagcgt tttttcttcg acttttggtg ctactgcggc accgatcatc tctacaggtt
     6361 tagcggtttc aatgttactt gtatccgcct tggcttctag cgcttctttt gtctgcgcgg
     6421 ctctattttg ctcttcaggc acgcctacat tgacctgcgc ttgacttcgc aacacgcggg
     6481 taatgttcat gcttttatct tcgtgaataa taaacttgcc cgcaggttga gtgattgtta
     6541 atgccgccat gtgaacgcgg tttggcgaga gtgacaactc caaactgtca ctggcgagtt
     6601 tttcccaagc taaaaatggc gcgtcaccgg cttcttcaag taaggctaat ttatcgacac
     6661 taaagccgcc attaaaggcc actgctagcg ctttttcgtc tttaacaatg agttttcccg
     6721 caacacttcc agcaccctca ttgagcttta gcaaggcaaa ttgatttaaa tacggtgaaa
     6781 atggttttaa agtgagtgcg gccagtttga ggtctaagtt cgccttcaac ggcgttggcg
     6841 taactttacc gaatgcttca aaacgtccac cctgcttaat attaaaactt gctttaattg
     6901 gcactgcgcg tgataaatcc aatgtggcat cacgtgcttc tagtgccaac ttatccacat
     6961 ctaataccac tggcacagca ttacttttat cctcaatatg cagttggcta ttttccagcg
     7021 ccagtttttt taaattaagc gaccaatcgt tatttttggt gggcttgctt tttacttgcg
     7081 ttactttttg cggtgtattt gcgtttgcac tcaaaattgt ttgccaattt aagggcgcat
     7141 cagccggctt aatgatttct gttttaagtc cagacaactg caatgaggca atattcactt
     7201 gctgtttatc aagcaaaatt tcacctttgc taagttgcaa tttatctaag ctcgccactg
     7261 gttttggcgt tgtgccagac ttcaccgttg cgttactaag tgccgtttgt aaacgatgca
     7321 atgtccaaaa accttctggt ttcttcagcg aaaaatttac attgatatcg gccacgtcta
     7381 cttgcagtga ctgcttaaag ctttgatctt gtaacgccgc ttgccaatgt tgcaaaccaa
     7441 tttcggcaat ttcaatggca aacgggctga caggcgtatt agctggtgtc gcgtcggtcg
     7501 ccaacgcggt gctttcagtg atgacttcat tcgcttcaaa cgcactttgc cagttaagtg
     7561 tgcctgactg atttctcgtt gcctgaataa tgcctttgcc taatacaact tgcgcgatgt
     7621 ttgcctgacg tttttctaaa tccaaacgaa tatgattgat gtcaaattgt ggcaaggata
     7681 acaaggtgtc tttaccatga gacacttgaa tatttgaaac tttgacatcc aaatcctcaa
     7741 attgcacttg tggatgatta ttatgctgaa caaacgccaa tttaggcaac gtaatgtgag
     7801 tacttgctaa ctgtacaacg gtttcttttc ctcgctgtat gcttagttca tcaagcgata
     7861 agtctagcgt ttgcactatt aaatcaggct gattttgata actaaaatct atttttggcg
     7921 ctgaaaattg cgccttagat aacgttacct cgccaacatg actaagcgtg ccggcaagtt
     7981 gattcaaccg gacagaaagg ccatttaaac tcacttttgg ctggtcatta tgcagcgcaa
     8041 aatcataaga aaaattcgcc tcaatatcac cacgacttgg cttgaacggc aactcaaccc
     8101 ctttaactat ttgcaaaatt tttgccagct tcaagcctga taccgccacc gcacctttag
     8161 acgcaactgg attaacaccc atatcaccct tccactttaa ggcgccgcca tgctcgggaa
     8221 acttcgccgc aattaagtaa tcgccgcggt ctttcggcaa agtagaaaat ccatccaact
     8281 caaaatctag cggactgagt gttgcttgat aaggcgccgt tcgatgggca tccatgtatt
     8341 gaagttgccc ttgttttacc acgatatgtg ctatcaccac gcgcggaatg gtttcactcg
     8401 gtggtttttt atcttcatta agtttttgaa ttaaatcagc ccaattgagc ttgccttgtg
     8461 gcgatattgc aaccaagcct tgcggtgcta atagattgat ttcattaaaa cgccacgccc
     8521 aatcaaacag cccactaaat tctaaatcaa ccaccagttt ttcaaaaccg gctaatggcg
     8581 caccatgttt atcagccaaa ttaaactgct caaccgttgc ctttaatcga aaagggtcaa
     8641 acgccacacg accaacactg gcctggcttg ccaactgctt atttgcaacc caaggcacca
     8701 gtttttttgc cactggattc accacaaaat aactgaatat caaataacct gcaaacagac
     8761 caacaaagac ttttgttggc aggctcacca ataatttttt tagcttattt ttcatgtcag
     8821 gtatttcaga atttttatat ttacaagcaa taacatcagc ttaacaaaat tctgccaaat
     8881 tgtattgctt ttaaaatgcc tattatttgt atatattaca caaatagact aatattatgt
     8941 atatattaca aattaaggga tgttaatgtc gctcggcaac tatattcgcc atttacgcaa
     9001 atcacatagg tttacattgc aacaactcgc gcaaagggtt gatgctgacg ccggcaactt
     9061 atctcgcatt gagcgtggcg aacttggcat atcagaaaac ctgttacgaa aaattgctgc
     9121 tgcttttgaa accaccccag caacacttta tgctgaaagt gataccgcac aaacgacccc
     9181 tgtgccggcg caacttgcct acaacaacgc cgtgcaaaac aactctcagg aatttgtgca
     9241 gtggtttcgt tcagcaacac cttacatcca cgcttttggt ggcaaaacat ttgtcattgc
     9301 ctttggtggc gaaattgtag atgaaaaaca attcaccacc ctcacacacg acctaaacct
     9361 attggccagc cttgaagtca ggctggtatt agtacatggc gcacgcccgc aaattgaaca
     9421 acgtcttaaa cgcgcccaac tcacaccctt attacacaac ggactgcgcg tcaccgatga
     9481 tgaggccatg gaagccgtga aagaggcgaa cggcgcagta aaagttgaaa ttgaagcgtt
     9541 actgtcgatg gggatcgcca attcgcccat ggcgggggcg gatattcggg ttgccagtgg
     9601 caattttgtg accgcaaaac ctattggggt catcgatggg gttgatttac agcatacggg
     9661 cgaagttcgc aaagtcaacg ccgcaggcat acaaaaacgt ctgaatgaag gggagttagt
     9721 attggtatca ccgctaggct actcgccaac aggcgaaata tttaacttaa gtttagaaga
     9781 cgttgctgtg agcactgcca ttgcactcga cgccgataaa ttaatatttt tgatggattc
     9841 tgaaggcgta cagaatttac gcggtgaatt gttgcgtgag atgaccgctg aaaaagcaaa
     9901 aaacctattg cgcaatgtgc aaaatagcga agaacctatc cacatttcag atgacattaa
     9961 atattatctg cctgccgcca ttcatgcatg tgaacatggc gttgcacgtg tacatttaat
    10021 ctcacgccat gttgacggtg caatcctaca ggaactattc accatggaag gtattggcac
    10081 catggtcacc gaattgccgc ttgaaaccat gcgtcaagca accattgatg acgttggtgg
    10141 tatattgaaa ctgattgaac cgcttgaaaa tgaaggcata ttagtacgcc gtggccgtga
    10201 gcgcattgag atggagatta accattttta tgtgatggag catgacaatc gcatcatcgg
    10261 ctgtgccgcc ctgtatccct ttatagaaga gcgggcggcc gaatttgcgt gtttggcaat
    10321 tgatcaggct tatcgaggag gtgggcgggg agataaattg tttgactact gcgcaatgca
    10381 agccaaagcc ttgggcttta aaaccctatt ttgcttaaca acacgcaccg aacactggtt
    10441 tttagaaaga ggttttaccg agcagagcgt cgataagttg cctgttgaaa aacagcaact
    10501 ttataacttt caacgtaaat ctaaggtttt tagtaaaacg ctataagtaa aattagttac
    10561 aatgctaatc atcaaaagtt gctataagaa aaataagcaa cgcttaaaaa tattcaacat
    10621 tcgagtgacg gaaaatctat gttaacccaa accactgccg caaaaaaagc acgaacgctt
    10681 gccgaagcgc taccttatat caaacgcttt tttgataaaa ccatcgtcat taaatatggc
    10741 ggcaatgcca tgacagacga acatttaaaa caatgttttg ctcaagatgt cgtgctgctg
    10801 aaattggttg gcatgaatcc ggtggttgtt cacggcggag gcccgcaaat taacgagatg
    10861 ctggataaac ttggcaaaaa aggtgaattt attcaaggca tgcgtgtgac cgacgaagaa
    10921 accatggatg ttgtggaaat ggtgctgggc ggtcaggtga ataaagaaat tgtcaacctc
    10981 atcaaccgtc atggcggtaa agcggtaggc ttaacaggac aagatggaaa cttcatacat
    11041 gcacacaaac tcatgatgga ggacctaaaa gaccctacca aaatgataga tgtggggcag
    11101 gtaggggaaa tcagcgccat tgacccaagc attattaact ttttagatag cggtgatttt
    11161 attcctgtag tagcccccat tggcgtaggt aaagatggtg aaacttacaa cattaacgca
    11221 gatgtggttg cgggcaagct ggcggaaatc ttaaatgctg aaaaattgat tttgttgacc
    11281 aatacaccag gtgtactaga taaaaacggt gagcttttaa cgggtttaac accgaatcag
    11341 attgaccagc tggttgcaga tggcacactt tccggtggca tgctgcctaa aatcagttca
    11401 gcattagacg ctgcgcgtag cggtgttaaa gctgtccaca ttattgatgg ccgtgtagag
    11461 catgccttgc tacttgaggt tttaacagat gaaggtgtag gcacgctcat caaggctaaa
    11521 tagcgtgcaa aagcgccaac aagaaaccat ttggattttt gatttagacg atacgctaca
    11581 caacgcttcg gcccatatat tcccggtcat gaatcgcaac atgactgaat atatgatggc
    11641 gcatttagca ctggatgaac atgatgcgca ccacctgcgt cagcattact ggcatattta
    11701 tggggcaact ttaaaaggat tggttcgtca ccacggcatc aatccccatc attttttaaa
    11761 cagcacccat gcaccgctgg atttagccaa catggtgtta gaaactaaaa ggcttaagaa
    11821 cacgcttcaa agactttccg gacgaaagtt agtgtttact aatgcgccgc tttcatacgc
    11881 catgcgtgtg ctgactatcc ttggtttaca ggactttttt gatgtcgttt ttagtgtgga
    11941 atctacgcat tttcacccaa aaccggcgat acttggcttt agcaaattac tcaaaaaatt
    12001 aaagccaatg cctcagattg tattatggtg gaagacagtc tgccagcgct tatgacggct
    12061 aaacggcttg gtatgaaaac agtttgggta accaaacagt taaaaaaacc aatttttgtc
    12121 gactatcgca ttaactcagt gttagcactt actcacattc agttataatt tagtctgatt
    12181 atttcgataa aactatttgt tggagagaac aatggcaggt gaacgtaagc agcaaattct
    12241 agaaacccta gctaaaatgc tggaagcgcc aaagcgtgaa aaaatcacaa ccgccgccct
    12301 agccgcaaaa ttgggcgtat ctgaagccgc gctctatcga cactttgcca acaaagcaca
    12361 aatgtttgaa ggcctgattt cctttattga agaaaccatt tttggtttaa tcaacaaaat
    12421 caacactgaa gaaactgacg gctatagaca aattcaacgc atcaccacca tgctactcgc
    12481 ctttggagaa aaaaatccgg gtatgacacg cgtactgatt ggcgacgcct tggtcaacga
    12541 agatgaaaaa ctgcaacttc gaattaacca actctatgac cgcattgaag tcaccttaaa
    12601 acaaagctta aaaatttccg ttgccacttc tggcaaaaag gtgatgcctg aagcgcaggc
    12661 aaacttactt atctgctatg tgattggtcg ttggcagcag tttgcaaaaa gtggctttaa
    12721 gcgcaaaccg ctggaatttg caagtgtgca agttagttat ttaaccaact aaacacgttg
    12781 gagaatcatc gtgcaaggca ccatttttgg cttaactgaa acacaaatta ccgaacttgg
    12841 catgtcctac gccgtgcctg gtctgatgtt attaatgcta ttcattgttg ggcacttagc
    12901 ttggcaatct aaggcaggta aatttggcac catgatatta tttctagcct tggcccttgg
    12961 tatggtaggt tatatcgcca aatctttaat tcaatataat ttgggcattt aaaccttggt
    13021 caatcttaag acaattccgc accaatattc gcccgtgcaa aaaaacatgc tctggaagcc
    13081 tttatttaca aggcttccag agcatgtcaa aagttgttat taaaactgtg ctgaatattc
    13141 taattttatg tgcgtcacaa tttctattta atcgcctcac ggtaaatcgt ttctctttca
    13201 ctaatttcag cctttgcgat gctggaaaat gttatttttg gcagcatttt tacttgccga
    13261 cggttggttt tcatattaac ttcgcgccag cccatcagta caaaccaatc ccataattcg
    13321 cgcgtgattg ggccagtgat ttgcattacc aatagctgcc tgccgatatg cgcacctacc
    13381 gctggcactt taccagctcg cggtgtgcgg cgacgttcat ggtctggctt acccccaaac
    13441 aacatgactt ttagcttatt taacataacg ttacacctct taataatacc gcttttactg
    13501 taacttcagc tcaaaacggt tagtattgta ttacaaaaat tttaattttg actgtatcgc
    13561 agccaatctc gcttcaaaga cggctacttt gattttttct ggagattcaa tcccacttgc
    13621 cacagcacct gcatctatac ttaacgccgc atccagcgcg tttaataacc ttagtgcttc
    13681 cggttgttca cgttcttcaa aacctgttct gccacgcgag tctgcctcac acgcttgcaa
    13741 aaagtgtaac agccgctcag gttgacgaat cgcatctaat tcaatgagga atatcagcaa
    13801 agtgctagga cgcatttctt ccactgcgtg taatttgccg tggtattttg caaccatttg
    13861 cgcgagttcc ttacacgctg ttggtatgcg tagtcgtttt gtaatatctt taagtaggct
    13921 cacactgcga gcttcgtggc caatatgacg tggcagcact tctttgggcg ttgtgccctt
    13981 acccaagtca tgcatcaagg cagcgaaccg caccggcagg ctaaaattct tgctggccgc
    14041 atagtcaatc accatcatga catgcacgcc ggtatcaatc tcggggtgat atttttctgg
    14101 ctggggaaca ccccaaagcc tttctaactc aggcaaaagt cgttttaaag cgccacaact
    14161 gcgtaatact tcgaacatgc ggcttggttt aacttccatt aatccctttg aaagctcttg
    14221 ccatacccgt tcagccacga gagcatccac ttcacctgcc tccaccattt gctgcatcag
    14281 ggcgtgggtt tcaggcgcaa ctgaaaaatc agcatagcgg gcagcaaaac gtgcaatgcg
    14341 taaaattcta acggggtctt ctgcaaaggc agtgctgaca tgacgcaggg ttttggagtg
    14401 tatatccgcc aagccgccat agggatctat caattgacca tcactacttt ttgccatcgc
    14461 attgatggtt aaatcccggc ggcctaaatc ctcctcaagc gttacatcgg tggcggcatg
    14521 gatggtaaaa cctttataac cgctggcaac tttacgttca gttcttgcta gcgcatactc
    14581 atcctgtgtt ttgggatgta aaaaaacagg aaaatcttta cctactggcc taaaccctgc
    14641 cgctaacatt tcatccacgc tgctaccaac gactacataa tctttatctg tcactgcaga
    14701 cccaagcagt tcatcgcgta ccgcaccgcc cactaagtat atttgcatgg ccgtatgtgt
    14761 attaaataat gcttatcttg cagcgtgacg acgatagcga tcatagtttc cgcgtgacgt
    14821 ttgattaact aaatattgcg gaatcacggt ctctaatgcc gatggcgtgc gggaaaacac
    14881 agcaggaaaa ggcatatcac aaacgctatc aacctccatc gaccgtacat tatcgcgtga
    14941 catcagttta atcggtaacc attccatgaa aaaagcctgc atatacgaca aacggtcatt
    15001 taagccaata attggccgct gaatttgcaa cacaatcata acttcctgaa ttagcgcgcg
    15061 gaatgtgtat actttggggc cagccagctc ataagtttga ccataagtag tcgaattttc
    15121 taaactggtc aaaaaacaag cactcacgtc ctcaacccaa attggctgaa atttcgcgtt
    15181 aggttttgca agtaaaatga caggtaaaaa tttaatcata cttgcgaata aattgatgaa
    15241 gcgatcgtgc cggccaaaaa taaccgaagg cctgaatatg gtgacattaa gacggatttt
    15301 ttcagccaac aacgccgctt ctccagcagc ttttgatctc aaataatgac tcggcgcgtc
    15361 tttatctgcg cgtaagctac tcatatgcaa cagacgctta atatttaaat caatgcaaat
    15421 tttagctaac tgggcaggca attgctgatg aattaaattg aatgtattgc ggcgactttg
    15481 atgcaagatg cctagtaaat taattaccgc atcggcacct ctcagttccc ggttcagcgc
    15541 tttgtaatcc aacacattgc actcaaccac acgcacattg ggtaataaca acaaatgctt
    15601 ggcagactca cgtcggcgcg tcagcacctt cacttgaaag cctgcagcgt ctaaccttgt
    15661 cacaatacta ctgccgacaa agccggagcc gcctaaaaca cacacttctt ttatttgcgc
    15721 aattgattgc atttttagcc tagcctatct ctacaaattg gattacttac tattcgcttt
    15781 cttcatcgta cacgaccgtt tgtccacttc ctggaacttt tcccaaacgt gattttaaag
    15841 tttgaatttt aacgccgggg tttttttcta acaaacgtgg agcatacatt tgtgcattgg
    15901 ccatcacctt ttgtacatat tgtctcgttt ccaaaaacgg aatactttca atataaatgg
    15961 ctgcttctaa cggctttgcg gccaaccatt tttttgcacg gcttggtcct gcgttgtagc
    16021 cggcggtggc catcaccgcg ttaccgttca ttaaatcaag cgcgtaacgc atatagtaag
    16081 taccaaaatc gatattggtg gttaaatcgt gaatcatgct gttgttgtaa ccacgtaacc
    16141 ctttacgttg cgccacccat ttcgccgtag cgggcatcaa ctgcatcagc cctgaagctc
    16201 caacgccaga ttttgcataa tgcataaaac ggctttcttg gcgggtaatg ccataggccc
    16261 aagcctcgtc tatatcgacc tcgcgcgagg aagtgcgaaa catttcacga tacggcgtag
    16321 gataacgcaa gttaaagtta tgcgtcactt tcgtattatc cgccgtgctt atggccacgt
    16381 cataccattt ttttctttgc gcgtattctg ccgcagctaa caattccgta tcagcaaaac
    16441 cgcgaattgc ccatgcccac tcagcctttg cctccatgcg catatccaag cgctgtaact
    16501 caagtgcccg cttgattcca gccaaattgg caattgcatt gacttcatca ttcgtcggca
    16561 caaaatctac tgatggatta ctcagcacac ttcccatgtc atctaacgcc agccaaccgt
    16621 aaaaatgccg ctcagtcgaa agcttactca acaattctcc agcctcggca tttttttctg
    16681 ggttattggc ttccaccact ttaatggcgc gcgccttcca ataacgccaa gccccttgct
    16741 ctaactgttt tggctgcatt gcatttactg catccagcac ggtttgccag tcctgatctc
    16801 gaagtgctgc gcgcactttc caagccaact gctcttcatc taatgatgcg ccttctgcct
    16861 gataataata acttactgcc tgcggatggt ggctgcgtgc tgcatgatac gcaatgcgcc
    16921 cccaaccaaa agcacgatgt tctgaatcaa tcgaattttg cactttttta taggcagcaa
    16981 ttgcattttg aatattattg cgcgctaggc gatcaaccgc atacagattc gcttctacac
    17041 caaatcggct tttaaatgac actggcggct tatctaaaaa tgcttgtggc gactgatagg
    17101 cacggtcgag caactttaaa tgtgccacat ctacactgct tagacgcgtt gccacgcttt
    17161 tggcaacacc gagtttacct gcatgtaaac ctaatctgaa tcttgcccaa atatcctcat
    17221 tggtcagcgc acctgatacc tgcaatgaat caaagacagg gttacattgg ctgggcaatt
    17281 ctgacggcgt tagccacaac atctttgctt ccgcagtcac atcggcattg cctaacattt
    17341 gatgcccttg cagcgcgtag caaagtaccg caatatcctc acgcttaaat agggtgtatt
    17401 catcaaaaaa gggtgcccaa ttttgctctt tacccagctt tttcaaccac tctccgcgta
    17461 cgcggtcggt aaatggcatg tcgatatatt gtgcgagaaa attttgcacc tcttcatcgc
    17521 gaaccgtatt ttgctgaagt ttcagaagca ttaaccagta atcagcgtaa ggggcaagaa
    17581 tgtactgctg gtctttgagt tggcttgctt cttctgcaag tgcaaaatca tttttggcag
    17641 cgtatgaggc gcgagcgcgc tgaaataacg cttcatcgga taaagcaaac gtttgacttg
    17701 agaatgtaat cgtgaccagc acgataaata ggcggatgtt gttcattggt tcgccccttt
    17761 aaaatgcgcg atgtgcttaa aatctgcaca aaaatttttt agtcttgcta acaaaattag
    17821 tactgttaaa atctctcaaa ttaagtgtaa aaacactcga gagaaaaata ctgccctgca
    17881 agcctttatt tataaggctc tcagagaatc gaaaaaggtt ggtgttatta tttacttaaa
    17941 aaactatttt ttttactgcg caataagcct gcttcaggca aatgccggat tgtgcatttt
    18001 ctcaacatct tctacttgtt gcgcgcttgg cgcatcttct ttattttcaa aagtggcata
    18061 ttcccaagct gtttcatctt gcatcagcgc gcgaatcagt tggttattaa gcgcatgacc
    18121 agacttgtag gcgtaaaaag cgcctaaaat cgggtggccg agcatataca aatcaccaat
    18181 cgcatccagc actttatgtt tagcaaattc atcatcgtag cgtagcccat ctgtattgat
    18241 gacacggtat tcatctagca caaccgcgtt gtcgaggctg ccgccacggg ccaagccatt
    18301 tgagcgcaag tattctactt catgcataaa gccaaacgtg cgtgcgcgac tgatttgctg
    18361 aatataagaa tctttagcaa aatcaatttt gacctgacaa ccagaatgct caaacacggg
    18421 atgattgaat gcgatggtga agtcaatctt aaagccatga tagggctcaa aacgtgccca
    18481 tttatcttgc tcttttactt ctaccgtttt tttaatacga atgaattttt taggcacagg
    18541 cagctcggca acaccggctt cttgcagtaa ataaacaaaa gggcctgcac tgccgtccat
    18601 aatcggaatt tcagcagcat ccacatcaat atacacattg tcgataccta agccggccaa
    18661 tgcagacatt aggtgttcga cggtggcaac gcgtgcgcca ttggcctcaa gcgctgagca
    18721 cagccgtgta tcatgcacgg cctgtgctga agccacaatt tcattgggct gaggcagatc
    18781 aacccgtttg aaaacaatac cagtattggc tgcagccggg cgcagggtaa gagtaacctt
    18841 ttcgccgtta tgcagaccaa cgccagtggc gctaatcgct ttttttaatg tacgttgctt
    18901 taacatgtag gtggtattgc ccttttcgtt cctcaacatt aaatacagac aaaaacattt
    18961 gtcgaaggtt taaatttatc ttacaaatca agcatatgat agcatatcta accagcaact
    19021 ccagtgtaat ggatgccgag catgatgatg catgcaagaa atattcaata aactcaagcc
    19081 gttttcgccg cgattagtct gcttgtttgc gtaaaaaagc tggaatatca tactcttcaa
    19141 cacccgccat tttcatggct tctgcttgtg cacgacggct gtttgagcca aacacctgtg
    19201 gtgcatcatc cgtcattaca cttgccgcgc caacatctgc acccatgtac atcggctggt
    19261 tggttgtgcc gttacgcacc acttcttgcg tcataactcg cagttcaggt ttttgttgac
    19321 gacgagatgc cccgtataaa ccagttgcca ccatcgtcac acgcaaacca tcccccatgc
    19381 tttcatcaaa cacattaccc acaatcaccg ttgcatcttc agctgtgaac gctttaatgg
    19441 tgttcattac atcgtaatat tctttcattt tgaaagaggc tgaagttgtg atattcacca
    19501 aaacgccacg cgcattcgcc aagttgacat cttccaataa tgggcttgcc actgcttgct
    19561 ctgcagcaat gcgtgcacgg tcaggacctg ttgcaacggc tgaacccatc atggccatgc
    19621 ccatttcact catcacagtg cgtacgtcag caaagtccac gttcaccaaa cccgggcaat
    19681 taatgatttc tgcaatgcca gaaacagcat tgtgcaacac atcattggcg gctctaaagg
    19741 cttctaagaa cggtacatct tcgcccaaaa cttccatcag tttttcgttt ggaatcacaa
    19801 tcagcgagtc cacatactta gacaattcct ctagaccatc caccgccact ttagtgcgtt
    19861 taccctcaaa agcgaaaggc ttcgtcacaa ccgctactgt tagaatgccc atttctttgg
    19921 ccacttgcgc aatcactggt gcggcacctg tgccagtacc accgcccata ccggcagtaa
    19981 taaagagcat gtcagcacca tcaatcaact ctgcaatggc attgcgatct tcaatggccg
    20041 cttcacgacc cacttctggc ctagcacctg cacccagacc tttagtcatt gacatcccca
    20101 tttgtagaat ggtttttgcc tggctctttt taagcgcctg catgtcagta ttggcgcaaa
    20161 tgaactcgac tccactgaca tttttttcaa tcatatgcgc gactgcatta ccgccacaac
    20221 cacccacacc aatgactttg atgacggctt cttgtgaatt tttttccata atttcaaaca
    20281 tgttgcttct cctctttttt gattaataag ctgcaccaaa atacagctct ccccatttac
    20341 ttctgccttt tactactact tgctactaca acttactact aaaaattccc ctgaaaccaa
    20401 ctcttcatct tttctaacat tcgcccaaat gaagttgatt ccaactgccc tgttaaatgt
    20461 ctttcgagct gttgcttacc cattaacact aggccaacgc cagttgcgta acgcgggtta
    20521 ctcaccactt ctgaaagccc accgacatac cttggcaacc ccatgcgtac tggcatatga
    20581 aaaatctctt ctcctagttc caccatgccg cgcatcatcg ccgaaccacc ggtaatcaca
    20641 atgccagagg caatcatgtc ttccataccg ctacgacgta actcactcag caccaactca
    20701 tagagttcta caattcgcgg ctctaacacc tcagccaaag tctggacgga taattgacgt
    20761 gcatctcggc catcaacacc tggcacttca accacctctc gcggatctgc caattgacgt
    20821 agtgcacagc catgtttaat tttgatgtct tcggctgatt gcgttggtgt acgaaaagct
    20881 accgcaatat cattggtcat ttggtcgcca gcaatgggta caacagcggt atgacgaatt
    20941 gccccttgct tgaatactgc aatatccgtt gttcctccgc caatatccac caagcaaaca
    21001 cccagttctt tttcatcttc agtcagcaca gctaagcttg atgccagcgg ctgtaagatt
    21061 aaatctgtca cttcaatacc gcagcgcttg atgcacttca ctatattctg cgccgcagca
    21121 actgcgcccg tgacgatatg cacctttact tctaatttca tgccactcat gcctagtggc
    21181 tcacgcacgt cttcctggcc atcaataatg aattcttgtg taagaatatg cagaatctgc
    21241 tggtctgctg gcagggcaat tgcccgcgcg gtttcaacaa cacgatccac atccatttgt
    21301 gagacttcgg catctttaat cttaaccatg ccatgtgagt tcaagctctt gatgtgacta
    21361 ccagcaatac ctgtaaatac attgttgatt ttgcaatccg ccatcaattc cgcctcttcc
    21421 aaggcgcgct gaatagattg catggtagat tcaatattaa ccaccacgcc ttttttcaga
    21481 ccacgagaaa tatgctgacc taagccgatg actttaacag cgccttctgg aagcaactca
    21541 gccactatca ccacaatttt agaggtgcca atatctagcc ctacaattaa atttttatcc
    21601 tcttttactc tgctcattta ttactccctg cgtgattgaa cacggcttat atctgttggc
    21661 taatatctgt tggttttggc ttcacttgca caactggttt ctttttattc gcgttaactt
    21721 ctttcgcttt ttcttccatc tgttttaagt ctgctgctgg tctacgcaca gcaaatccat
    21781 ttggatagcg caaatctgca taggcaattt ttttattcaa cccagcgatg gtacttacat
    21841 aaacatttgc gaatttttca attctgtttt gcatttccac tcggcctaac tcaatgtgca
    21901 ttccacttga agtggtgact tcccacgcgc gcctaggcgt gagagtcaaa ctggtaacct
    21961 tcaaattggc ctcctgtaac gctttactaa aattgccaaa ttgcgaggcc acctctatta
    22021 cgctgtctgc agggccatta aatactggca actcgctgtt tgatgccgca tgaaatagct
    22081 cgccgtgtgt attcactaac gcaacgcttc cccatcttgc aagctcctga tgctcttcaa
    22141 ctacaacttc taatttatca ggccaacggc gacggatact cacgttacgt gcccaaggca
    22201 acttttcaaa agcagtgcgc gccttcatta aatccaatgt aaaaaaattg ccctttaaat
    22261 gcttactcac aattaacttc acctgctcac gactcacatg gttcaactgc ccatcaattt
    22321 tcacttcacg caaaggaaat attggcaaat gcaccaccgc atatagcact gcataaagaa
    22381 tcgccaccat acttaatgca aacagtaagt tggcaatcca attcaataat ttgggtttat
    22441 cccacatcgg ccaattccaa aattcgtacg accaaatcct caaaactaat gcctgccgct
    22501 tttgcggcca ttggcactaa actatggtca gtcatgcctg gactcgtatt aacctctagg
    22561 aaataatgtt tccctgaagc atccattaaa aaatcaacac gcccccaccc tctgcaacca
    22621 attgttttga aagcaattaa agcttctttc tgaatttgtt tttctttttc ctcacttagc
    22681 cctgatggac ataaatactc agtgtcatcg cgtaaatatt tcgcttcaaa atcgtaaaat
    22741 tcattttttg gcacaattct aatgattggc aatgcagtat agccaccagt cgcatcaccg
    22801 ataatgccta cggtatactc gccaccgccc acaaactttt cagcaatcac caatgggtct
    22861 gcttttgcag ccaattcata agctgcctgt aaaccgcctg cagttttcac cttactaata
    22921 ccaatactcg atccttcatt ggcaggtttc acaaataagg gcaatcctag ttttgtttca
    22981 attgccgcaa agtcactatt tgcagtcact aattcaaagt ctggtgtcac gacattcatc
    23041 gcacgccata acaatttggt acgccattta tccattccaa tgctcgaggc catcacgccg
    23101 ctacctgtgt aaggaatatg aagcatttca agtgcacctt gcatgcaacc atcctcacca
    23161 aatctgccat gtaagcaaat aaacactcga tcaaaactct ccaaatcatg tagcggctta
    23221 tcacgcgggt caaatgcatg cgcatcaatg cccttgccct tcaatgcggc caacaccgca
    23281 gctccactct tgagtgaaac ttcacgctca cctgactttc cacccagcaa cactgctact
    23341 tttccaaaat ctttacgcat attcaccaat cacccgcact tcttgttgta acgctatccc
    23401 aaatttatcc atgactgttt cttgcatgtg cctaatgagc aattcaatat ccaatgccgt
    23461 tgcagttcct aaattcacaa tgaaattggc atgcttctca gaaacttgcg ccccgcccat
    23521 tacataacct tttaagccac tcgcttcaat caaccgcgct gcgtaatccc cttcaggatt
    23581 tctgaaggtt gagcccgcat tgggcaggtt caaaggctgt gtcgccaacc gctttgctag
    23641 tagcgcttta attttctgct ccgcctcatt catgtcccct tcaggcaaat caaaccatgc
    23701 agctagaaac cattcttctg cgggcatctc aacatggcga taactgacgc gatactctgc
    23761 tttattgcgt gagcgaatca caccttgcct atctactgtc atcactttgt tcaccacatc
    23821 ccatgtttct gcaccatagc aaccggcatt cattgccaat gcgccaccaa tagttccagg
    23881 aatacccgct aaaaactccg ccccatgccg atgttgtttt gcactaaatt ttgcaacttt
    23941 ggagcaagtc actccagatt ctgcataaag cttgttatcc accatcccca tatcggctaa
    24001 tgcgttgtgc atcaaaataa ccgtgccacg cactccgcca tccctaacca ataaatttga
    24061 gccaagccca acaaaataaa cgggttcatc tttatccaag ctttgcagaa atgctgcaac
    24121 atcttccaat cctgcaggga cataagcctt atccgcaata cctccgacgc gccagcttgt
    24181 atagcgtgcc atcggctcgt tcattaacaa cgtaccttgc gttaacacca aatccccctg
    24241 caaaatatgg cgctttgcat tcacttaagc catacctctc gttctactgg caacttggcc
    24301 aatagatccc gcacccatca caatcacaac atcattcggc tgtgcaatac ttaaaatggc
    24361 ttctggaagc gcatctgttg tttctacaaa gataggttct acttttcctg ccacgcgaat
    24421 ggcgcgaata agcgatcgtg tatcggctgc aataatgggc tgttccccag ccgcgtatac
    24481 ttcagtcaac aagaccacat ccgcgcttga taaaacgcgt acaaaatctt caaaacaatc
    24541 tctcgtgcgc gtataccgat gcggctgaaa cgccatcact aatcgacgat ttggaaatgc
    24601 cgcacgggca gctgcaatga ctgcctgcat ctccactggg tgatgaccgt aatcatcgat
    24661 taaagtaaaa gtgccttttc cgcttgacgc gcttacacta atttcaccat aacgctcgaa
    24721 acgtctacct acccctttaa actcttttag cgccttgatg attgcctcat ctggcacatt
    24781 gagttcactt gcaatcgcaa tcgctgccaa agcgtttagt acataatgat tgccaggtaa
    24841 attgagcgtc acatcaaatt cagtgatcac accgttgata cgctgaacgc taaagtgcat
    24901 ctttccatcg tctgcacgaa cattaatggc tctcacttta gcctcttcac taaagccgta
    24961 agtcattacc ggtttcgtga ccctaggcaa aatttcacga atatttgcgt catccacaca
    25021 aacaactgcc ataccccaaa atgggagctg ctgtaaaaat tctacgaatg cacttttgag
    25081 cttgtcaaag ttatgctcat aggtgtccat atggtcttgg tcaatattgg tcaccaccgc
    25141 catgaccggc gttaagtgta aaaaagaagc gtccgactcg tctgcttctg caacgatgta
    25201 ctcgccagtc cctagttttg cgttggcatt tgcggcctcc aatttgccgc caattacaaa
    25261 ggttgggtcc atgcctgcct cagccagaat gctggcgatt aaacttgttg ttgtggtttt
    25321 gccatgcgtg ccagcaaccg ctattccctg ccgaaaacgc attaattccg ccaacatcaa
    25381 tgcgcgcgga acaacaggga tactcttagc ccttgctgct ttaatctccg ggtttgcttc
    25441 attcacggca gaagatacga ccaccacgtc tgcatcacct aagttttctg acacatgtcc
    25501 ctgatagacc gtcgccccaa aatctaccaa acgcttcgtt gtggcattca cagctaaatc
    25561 tgagccacta acactgaaac caagatttag aagcacctcg gcgatgccgc tcat
//
LOCUS       DAOQSI010000013        25552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_1212_length_25552_cov_6.321450, whole genome shotgun sequence.
ACCESSION   DAOQSI010000013 DAOQSI010000000
VERSION     DAOQSI010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 25552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25552
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1212_length_25552_cov_6.321450"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..974)
                     /locus_tag="PKL42_01915"
     CDS             complement(63..974)
                     /locus_tag="PKL42_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65855.1"
                     /translation="MQNAFKTDPFDQYLLKARGDKLIRIHISRNTLIAVIFSLLVHAL
                     ILSIVVPKIQLDAASAKPPTAFEVSLAPPKLPEEIVAPIEKPVQPEEKPAPKTIKKTT
                     KKIITKKAVKTEKPVLTVTEELAMPIPVPTVAEKPVEEPPTTEAPTDMASYVRQQQAK
                     RNAQEADAARQNEEAIAREQGPSAEQLRDERIKNNFKNGTNGIFEISSLSGRRATFSF
                     KGWTNDYSNARRQFFEVEASGGQDVRLVMVKKMITLIREHYQGDFNWDSQRLGRTIIK
                     SARIEDNTELEEFLMMEFFGTHYKTKF"
     gene            complement(981..4958)
                     /gene="hrpA"
                     /locus_tag="PKL42_01920"
     CDS             complement(981..4958)
                     /gene="hrpA"
                     /locus_tag="PKL42_01920"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HNU65856.1"
                     /translation="MLVDRHALRRKLRDVADLQKIGDAQANGKAQRLLGEVAQKMRAS
                     QAKYAARLANLPKPDYPLELPVSGKKDEIAAAIAKHQVVIICGETGSGKTTQIPKICL
                     ELGRGVSGLIGHTQPRRIAARSVASRIAQELHSPLGEVVGYKVRFNDKLSESTYVKLM
                     TDGILLAETQGDKFLNAYDTIIIDEAHERSLNIDFLLGYLKQLLPKRPDLKIIVTSAT
                     IDADRFSSHFNGAPVIEVSGRTFPVEIRYRPLGAAGFRAKESAEADNAQFDLDDDTIF
                     GIARKAKTEARWLEEDDEEEAIEEAIMDAADDLLRQGDGDILVFLPGEREIRDVADHL
                     RKYQGRSAKLKHIEVLPLFARLSIEDQQKIFKSHSLRRIVLATNVAETSLTVPGIKYV
                     IDAGLARVNRYSSRAKVEQLQIEKISQAAAKQRAGRCGRVSSGICVRLYSEQDFNGRP
                     EFTEPEILRSSLASVILRMAALRLGDVAEFPFIEAPSTRLIADGYLLLQELGAVDARR
                     QITETGLQLAKLPLDPRVGRMILAAKREACLKEILIIASVLSIQDPRERPMDKREAAD
                     QAHAKFGGEGSDFMSYLKLWDWFDAALNTKKSNKDLLNQCHSNFLSFLRLKEWRELHG
                     QLLEIVSEMDFKRNEKEANYEQIHKALLAGLLGNIGFKDGESESYAGARGIRFHIAPG
                     STLKKTRPKWVIAAELVDTTKLYARCVAKIEPDWIEPLARGLTESHYSDPRWDRKMGM
                     VNAWERVSLYGLTIIRKRRVHYGPINPVESREIFIREALANGEFDTRAPFFMANERLI
                     AEVEELEHKARRQDVLVDEHQLFSFYDAKIPADIVNAASFEKWRTEAEKLNPKLLYLT
                     RDDLMRHGADAITAVQFPEKILLDHVETPLKYRFEPGHVLDGVTATIPLALLNQLNPV
                     QTEWLVPGMLREKLTYLIKALPKTFRRVCVPVPEFVTGFLESVDYQFSTPILETLASY
                     IQHKTTLKVSKDDWDLNDIPPHHLMNFSVIDDAGRELAMARDWNALKKQLGSAAQLTF
                     RNTSPDIEKTGLKQWDFGDLPQTLSFERDGLKVVGYPALEDEIESVSVKLFDTEREAV
                     ANLRKGVCRLMRFELKEQMKQLEKSLPNFNQYALTLRGVMSPEDLREDMLLTIADRAF
                     IGEDDLPRTNDDFMKLKARARTRLPAVTQAVARQVQAIATEYQLLVNQQAKMPATVNR
                     LKRDVEQQIYLLVYKGCFSQTPWEHLQHISRYLKALRLRIEKHPANPDRDGKNAASVG
                     QIWQKWQDKVSALQQAHHDIPQALQDFRWLIEELRVSLFAQELKTPFPVSIKRLEKTW
                     AEMHF"
     gene            5130..5765
                     /locus_tag="PKL42_01925"
     CDS             5130..5765
                     /locus_tag="PKL42_01925"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNU65857.1"
                     /translation="MLIPNNLPFERKLMRAVLKQHAGIFLLLLCMSLIFAGIGAAVIY
                     FKAAEDGGLYIFGGLFLLAGISIFIFNLISNYSSVQYYYEKGLLKQYGREVNATITDK
                     RVDDAIVKNSPNSSDADTTTERDCIIAYSYVFGGKKYDAESFLDSQALFDQLEIGQRI
                     PILVLPQQPETTYPRIKKLKAMLKMNLQQAKKSVENGTENIQVSQGLMEEE"
     gene            complement(5954..6805)
                     /locus_tag="PKL42_01930"
     CDS             complement(5954..6805)
                     /locus_tag="PKL42_01930"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HNU65858.1"
                     /translation="MRLQSFFRLLSSTFLVLCFAQAALAQQNNADILLAQVYQRGIAV
                     EHYLVSEKYDGVRALWDGKTLRSRAGNVIAAPIWFTEKFPNTPLDGELWLARGKFDAL
                     SGAVRKAVPVEEEWRGISYLIFELPDAPGTFAARVQRINDIVKVANVPHLKAVTQFRV
                     KNEAALEQKLKQVVATGGEGLMLHRADAPYVTGRSDVLLKLKLELDAEATVVAHTAGR
                     GKYQGKLGALVVETPDGVRFKLGTGFTDAQRENPPKVGSVVTYTYRDKTKHGKPKFAS
                     FLRVRQE"
     gene            complement(6812..7657)
                     /locus_tag="PKL42_01935"
     CDS             complement(6812..7657)
                     /locus_tag="PKL42_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNU65859.1"
                     /translation="MQTIPESLKADVRPHQSVELLKALHILTRDGKLNLDSRRKLKQV
                     YHLVNFIEPLFNAVLAHNANPTLVDHGAGKSYLGFILYDLLMKQNERGQVIGIETRAD
                     LVEKSRQLAANCGFTRMHFEHLTVEESITSAAIPAQVDIVTALHACNTATDDAIQFGL
                     QKHAQYLVLVPCCQAEVAEVMRQHKSASLGKTALSEIWRHPIHTREFGSQITNVLRCL
                     QLEAAGYNVTVTELVGWEHSMKNELIIAKYTGQPRKNALERMQAILHELNLETMQSRF
                     ALPNR"
     gene            7988..8482
                     /locus_tag="PKL42_01940"
     CDS             7988..8482
                     /locus_tag="PKL42_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNU65860.1"
                     /translation="MPNKNISKLSLIHAKNPVLSRGFTVVELMIAVAIVGVLAAIAIP
                     AYSGYQEKARVYQAVTDVAAMSVTIENYFQENRIYPETLADVKLDGKLDPWDKPYRYL
                     NLVKNGNGGARRDKNLVPLNSDFDLYSEGEDKKTKLPISQKDSLDDILRANDGKFVDL
                     AEKY"
     gene            8506..11334
                     /locus_tag="PKL42_01945"
     CDS             8506..11334
                     /locus_tag="PKL42_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNU65861.1"
                     /translation="MQISGTFLNSKVAQRIALLLLIAAVIPAVLITWLTYRNVDHLTN
                     QHSQQTLKATSQNYALLAFNNLLTARKFLHYATQSYHHQQHHAEEIWQHAQSITMFNA
                     IVEISADGKRISQHGQYQDLSEAIKKQSSSIISSATNDKTRLIVFPAKLAQQSAHVNL
                     AMPIFNEGQLESVLIAELNPDYLWGLKSNYPSDQKICAYRVTDTSKTTLFCSTDALDI
                     AHQTSQTGGWDLFLKAELHDAPWTFETTQFTPAAKSGLWALIGSNAYIGVGLLSLLIV
                     GLLSLIQIRRTMIPLERLIDSTRKISHGQFESVEVDGKSEFSELATAFNSMSGNIKRQ
                     LDTLQSLSEVDRKIVTKLDIDHLIEQVMARILHLKPEVSASIFRIDEHNDDEKTESEI
                     QCIVDIAQVANASTLRKTITSQEASYVKTLNNGVILPCDAKSQFTHQQMMASLGANTI
                     WLLPIFWQGEMCAFLVIGSQQKLDEHDSDLHEIRDLASRIGIVISAQKREAQLLLQAH
                     YDILTGLPNRILLEDRIGQAIEHSDRTKLPMWVVFLDLDRFKFINDSMGHNIGDQLLV
                     QVAHRMQAAVREVDTVARFGGDEFIIVLPGQTDETLMMGVLERIIHMVCQPMQINNQE
                     IIITCSVGIAVYPNDGAKAETLIQHADIAMYRAKELGKNNFQFFTESMNEKVAERMYV
                     ENLLRHALAKNELEVYYQPKVDLETMQMVGMEALIRWNSPELGFVSPIRFITLAEESG
                     LIVPIGEWVMRSACQQTVAWQNAGYAPLLMSVNLSARQFKQSNLVQRIQNILKETGLE
                     AQYLELELTESMVMTEVEASLQLLHEIKTLGIQISVDDFGTGYSSLSYLKDLPVNTLK
                     IDKSFTDDIINHTDKAPIVQAVISLAKSLNLKIVAEGVESYEQVLYLQQHACDQMQGY
                     YFSRPKPAVEIEKLLIEAQATAPKLAHA"
     gene            11732..12103
                     /locus_tag="PKL42_01950"
     CDS             11732..12103
                     /locus_tag="PKL42_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65862.1"
                     /translation="MKKTIILTATLAALTTSFSALADRDHRLDQRGDRIENRLDQRGD
                     RIEDRLDRRGDRIEHRHDKRADKARAHGHDKRADHLERKGDRIDQQLDDRGDRIDDRL
                     DRKGERINRRLDRRGAHHAKK"
     gene            12177..12695
                     /locus_tag="PKL42_01955"
     CDS             12177..12695
                     /locus_tag="PKL42_01955"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNU65863.1"
                     /translation="MQHFLAEIEGRAFRIAQIATGNREDALDIVQDAMIKLVQKYSTH
                     AKDEWKPLFYSILQTRILDWHRRQNVRNRFRSWFHWDEDEDTEDFLEQQPANNASAPD
                     LQLQDDQFMDNLNTALSKLPLRQQQVFLMRVWEGMDIAETAAAMQCSQSSVKTHYARA
                     LEKLRAQLEHHQ"
     gene            12709..13113
                     /locus_tag="PKL42_01960"
     CDS             12709..13113
                     /locus_tag="PKL42_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65864.1"
                     /translation="MNNHQFEDKVKASLTRSVEAIDADTRQRLADIRRQSLNTASEKV
                     SPAKWLTLDFWRGNHLMPAASLAFCALIAVFLVSSPQTQNPTNVIPSMDNQNQVAILE
                     LLNNPDELEVLSDPDFYLWADEMLMDETDNAV"
     gene            13103..13336
                     /locus_tag="PKL42_01965"
     CDS             13103..13336
                     /locus_tag="PKL42_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65865.1"
                     /translation="MLCRFLLATSFLCLTANLQAQEPAIPLDLIELLGEFDAEDTDSI
                     KEAVTQVGTNPQKNKPSVKQVGQAKTTSAEVRK"
     gene            13376..13888
                     /locus_tag="PKL42_01970"
     CDS             13376..13888
                     /locus_tag="PKL42_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HNU65866.1"
                     /translation="MHKRFNSRLKFSAITLSLVISLMSLTARAHSDDAEGVAWENLSA
                     QQQETLSAIKENWGTLPAERQRRLSKGAEKWGAMDPEQRAHIQERLHHYQNLSPEDKE
                     VVKEQMRAFKKLPSDERKALRERWKNLPPEQKEMVRERYKDMNHDERKAMREAYKQKK
                     REWRKHREHD"
     gene            14084..15034
                     /gene="ylqF"
                     /locus_tag="PKL42_01975"
     CDS             14084..15034
                     /gene="ylqF"
                     /locus_tag="PKL42_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HNU65867.1"
                     /translation="MAIQWYPGHMTQARKKAEETMEFTDVVIEVLDARVPVASHNPMI
                     DEMRLFRQRPQLKILNKSDLADPKATQAWLNYYNKQPNTKAVALSCKKAGEANKIPKL
                     CLGLAPHRGTHLKPLRMLIMGIPNVGKSTLMNALLNRRVAKVGDEPAVTKSQQRFDLS
                     DTMSITDTPGMMWPKIQYESDGYMLAASHAIGRNAVIDEDVALFLANNLLLTYPALLN
                     ARYKLDTMKHEGAMLDVLKMDGVDLLEAIARRRSYKRHDGLYDMEKTAVAFLTDYRSG
                     AIGRVTLETPESRSAMMQSTQAVNEAETTENEAPDETNAD"
     gene            complement(15066..16376)
                     /locus_tag="PKL42_01980"
     CDS             complement(15066..16376)
                     /locus_tag="PKL42_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65868.1"
                     /translation="MMNDSIHKTASGNVVYLELVDFSDKTDAEQIDLKSKFNALVKVA
                     LKVPQTHTRIVTDTVEGVIVTTLGAPEEVLQIAANMQNIVHQYNIRTTAPVLVGFALH
                     SGQVQIVNDIHDQPQVLGDAIKLAQRIASFAQPNEMLATQAFVAATAHLMQGETNVID
                     YAGILNERHDLGLPLYAMHFTEGLAALNKAFALQGPAPIVDIKHENTEPPIEVLAEFK
                     GVHWPYALSGILILVGLYALVQTLAIPNEPTITMTSAETIEPPAPVAVKQPEVKQPTV
                     LDPYGLLPNETIEELPAPQQKSEEVVAPVIKPEKTIRAVSQIADEESEKLARKVFAEQ
                     LKLEAQSQAKTESAPVVEQNKLPEVTLSATETKAEATEAAKTSELPPKEEHVKQPEAV
                     APKVEPQLQTKDNEKSGWGVFTGGLKKGQVRTCSQGEIALGQCR"
     gene            complement(16533..16787)
                     /locus_tag="PKL42_01985"
     CDS             complement(16533..16787)
                     /locus_tag="PKL42_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65869.1"
                     /translation="MRSTRSTKASAAIDRLKNRSGNTQYSMTLHQGALFSLCVAGEAT
                     RLCEPMPLDDFVAFVNAYGPQTPKRVSALDIAFNKQLVKK"
     gene            17141..19174
                     /locus_tag="PKL42_01990"
     CDS             17141..19174
                     /locus_tag="PKL42_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNU65870.1"
                     /translation="MLNSLNSPQREAVKYLDGPLLVLAGAGSGKTRVITQKISYLIDE
                     AGYSPKEIAAITFTNKAALEMRERVSKLMQGKSIKGLTIATFHSLGLQMLRAEATLLG
                     YKPQFSILDSSDSFKILSDVLATTDKQLLRKTQWQISSWKNAFINPDQAKATADEELT
                     HAAAKVYQIYQQTLKAYQAVDFDDLIKLPVELFEQHDEALNKWQRKLQYLLIDEYQDT
                     NACQYKLVKMLTGVRGQFTAVGDDDQAIYGWRGADVENLRQLTEDFSKLKVIKLEQNY
                     RSTVRILRAANQVISNNPKLFEKKLWSDLGTGDMLQVTATMSEEHEAESVVMKLQAHK
                     FEHRTKFMDYAILYRGNHQARIFEQHLRNQKIPYTISGGQSFFDKTEIKDLISYLRLI
                     ANEDDDPAFIRAATTPKKGIGNTTLERLGEYASAHKISLFSAAFEGDFQSEIGHKQLD
                     DLLNFCTYIQKIQARAVRDPAGDVLNDLLTSIQYEAFLYDTDEPRAAEAKWANVMDFI
                     GWLTKKGEANTEFGNEAEGKNLLELTQMVSLMSMLEGRENGEPDAVKLSTLHAAKGLE
                     FGHVFLIGVEEGILPHRESVDAATADPSRIEEERRLMYVGITRAQKSLNISWCKKRKR
                     AGEMEMCEPSRFIAEIPKDDVRHFGNPFNKDPEARKDFGKSKMANIRAMLGKG"
     gene            19318..23007
                     /locus_tag="PKL42_01995"
     CDS             19318..23007
                     /locus_tag="PKL42_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNU65871.1"
                     /translation="MNIGRKLMLIVASSVALVTIPAATGIYFYVAHNLLASEAATQVA
                     ETSTLVMANTQKVQSYEIGLQSLSKTLTNTLSAPPLAGEEAAFDAIIQRHPDGAWRNK
                     RATFNGKEESGLFLPPDASMDIHQKRLHFRTKQVLDAFGSSITTPTGNIWVLTHDKTE
                     IIFDHLYPDFTMLMAADTDYTKTPWMTLGDPAINPKREVRWTSPIQDPVTKSWIISAI
                     LPLDINGRWIGTIGRDIGLNVDSLSIFQKHEHFPGELRFLLDAERHFIEAGPWQTLLQ
                     TNPQAIQSHLANEPALAELLNASTATASAYDKKVTIQGRQYLATSMVLPVVGWHYFRL
                     VPQDEILKPMQQLFYTLTALILAIGLLIGSLIEIAVQRQIISRIKVLASTLRRYSLGD
                     LSARTNLKGNDEIAQVSHEFDEMAGQIKSTLDALPDILFDLDLNGQYYAVHTPDEKLL
                     TAPVKELIGRTVPQMLPQHAAAIVMAALKEANQKGWSRGMQYLRKTPIGTFWFELSVA
                     KKTNSDFDHPRFIVLSRDITERKQFESRLSESEFRWKFAVEGTGDGMWDWNIQTNEVQ
                     YSTRWKSILGFAEEEIEPTREAWKKLVHTEDIAEVMQAISEYLTGRTTTYTIEYRMQS
                     KNGQYLWILDKGVIVNRDAIGKPLRMIGTHTDITERKTIEHDLRISAIAFDAQESMMV
                     SDVNKVILRVNKAFTAITGYAAEEVIGHTALPLRSNQHEASFYKAIWEGVDANGAWKG
                     EIYSMRKNGDVYPGLLSITAVKNNQGAITNYVTTLVDITKEKTTAQEIQFLAFYDPLT
                     GLANRRLLLDRLSHALVAHNRSGRDGALIFLDLDHFKSLNDTLGHDVGDMLLKEVANR
                     LTACVREDDTVARLGGDEYVVLLENLSEKAIEAATQVEAIGTKILAALNKPYQLGTHE
                     HHSTPSIGAALFSDHTETQEELLKHADIAMYQAKKAGRNTLRFYDPQMQQAIHARVSL
                     ERELRKVIEKQQLRLYFQIQVNSDGEPIGVEGLVRWLHHERGLIPPLHFIPLAEETGL
                     ILPIGQWVLNAACAQLKLWQNMALTQDLILSVNVSAKQFRQNDFTTQVKTAVEQHGID
                     PTKLKLELTESILLEDFEGTIATMNALKEIGVLFSLDDFGTGYSSLQYLKRLPLSQLK
                     IDQSFVRDIFTDNSDKAIVSTIIAMANSLDLEVIAEGVETKEQQQLLMDIGCKAYQGF
                     LYGKPVDIQQFEAALKNTLVDPRLV"
     gene            23145..25064
                     /locus_tag="PKL42_02000"
     CDS             23145..25064
                     /locus_tag="PKL42_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNU65872.1"
                     /translation="MPYITLDNASLAFGHHALLDHAAFQLDAGERVGLIGRNGAGKSS
                     LLKAIAGTIKLDEGTVWRAPNARVVYVPQEPELDTTHTVFEAVAEGLGTLQQTIIDYH
                     QVTHDMGMPDADIEALMTNMQHLQHEMDAQNGWAAQSRVETVLSRLNLDADALISTLS
                     GGWRKRVALGRALVAEPEVLLLDEPTNHLDLEAIEWLENLLLGFNGSVLFITHDRRFL
                     DSLATRITELDRGKLTDFIGNFTQYQAKKEELIAVEETHAAKFDKVLAQEEVWIRQGI
                     KARRTRNEGRVRRLEALRLERAARRERQGNVKLNIDAGERSGKLVAELDNVSKSYGDR
                     VLIKNFSTRILRGDKIGFLGPNGIGKTTLLKLILGEIEPDSGSIERGTKINVAYFDQM
                     REQLNEEATLADTISPGSDFVEIGNERKHVISYLEDFLFPPQRSRSPVKSLSGGERNR
                     LLLARLFARPANVLVLDEPTNDLDIDTLELLESLLQEFTGTLFLVSHDRAFLENTVTQ
                     VIAFEGDGKLTEFGGGYDDWQRFTQQRQLNQKAVDKKAEATANKTKSQATETPPKSAS
                     KLSFKEQKELDEIPLKVEQLEAEQTTINTQLADSELYKTQATLVKTLQSRLNSIDLEL
                     ESLLAQWEKLEAKKA"
BASE COUNT         7261 a         6016 c         5641 g         6634 t
ORIGIN      
        1 agctatattc gccgtgtatt ttcttaatcg gcaacaactg taagaacgct gactaacatc
       61 cttcaaaatt ttgttttata gtgcgtgcca aaaaactcca tcattaaaaa ctcttccagc
      121 tccgtgttat cttcaatgcg tgcagattta ataatggtgc ggcctagtcg ctgcgaatcc
      181 cagttaaagt cgccctgata atgctcgcgt atcaaggtga tcattttttt caccatcact
      241 aaacgcacgt cttgcccgcc actggcttct acttcaaaaa actggcggcg tgcgttgctg
      301 taatcgttcg tccagccttt gaatgaaaag gtcgcacggc ggccacttaa gctggaaatt
      361 tcaaatatgc cgttggtgcc atttttaaag ttatttttaa tgcgctcatc gcgcagttgc
      421 tcggcactcg gcccttgctc acgcgcaatg gcttcttcat tctggcgtgc ggcatctgct
      481 tcttgcgcat tgcgcttggc ctgctgctgg cgcacataag acgccatgtc ggtgggcgct
      541 tcagtggttg gtggctcttc tactggtttt tctgcaactg tgggaactgg tatcggcatg
      601 gccagttctt cagtaaccgt taataccggc ttttcggttt taaccgcttt tttggtgata
      661 atttttttgg tcgttttttt gatggttttt ggcgctggtt tttcttcagg ttgtactggc
      721 ttttcaattg gggcgacaat ttcttcaggc aattttggcg gtgcaaggct cacctcaaat
      781 gcagttgggg gtttggccga agccgcatct aactgaattt ttggcacaac aatgctcaaa
      841 ataagcgcat gcactaataa tgaaaaaatc accgcaatca gcgtgttacg gctgatatga
      901 atgcgaatta acttgtcgcc acgcgctttg agcaggtatt ggtcaaacgg gtcagtttta
      961 aatgcgtttt gcatacgccg ctaaaagtgc atttcagccc aagtcttttc aagccgttta
     1021 atagacacgg gaaacggcgt tttaagctct tgggcaaaca atgacacgcg taattcctca
     1081 atcagccagc gaaaatcttg caacgcttgc ggaatatcgt ggtgcgcctg ttgtaaggca
     1141 ctcactttat cttgccactt ttgccaaatt tgcccgacgc tggccgcatt tttaccgtca
     1201 cggtcagggt ttgctgggtg tttttcaatg cgcagccgca gcgcttttaa ataacgcgaa
     1261 atatgctgca aatgttccca cggggtttgg ctaaagcagc ccttgtacac caataagtaa
     1321 atctgttgct caacatcacg cttgaggcgg ttcactgttg ctggcatttt tgcttgctga
     1381 ttaaccagca attgatattc cgttgcgata gcctgcactt gccttgccac cgcctgcgta
     1441 accgcaggca agcgcgtgcg cgctctggct ttaagtttca taaagtcgtc gttggtgcgc
     1501 ggtaagtcat cttcaccaat aaaagcgcgg tcagcaatcg ttagcagcat atcttcgcgt
     1561 aaatcttccg gcgacatcac gcctctcaaa gtgagtgcat attgattaaa atttggcagg
     1621 cttttttcaa gctgcttcat ttgctctttt aactcaaagc gcatcagccg gcaaactcct
     1681 ttacgcagat tggctactgc ttcacgttcg gtatcaaaca atttcactga aacactctca
     1741 atttcatctt caagtgctgg ataaccgaca actttcaagc catcacgctc aaaactcaag
     1801 gtttgcggta agtcgccaaa atcccactgt tttaagccag ttttttcaat gtcgggtgac
     1861 gtatttctaa aagtaagctg tgcggctgaa cccaattgtt tcttgagcgc attccaatcg
     1921 cgtgccatgg ccagctcgcg cccagcatcg tcaattacgc tgaaattcat cagatgatgt
     1981 ggtggaatgt cgtttaaatc ccagtcatct ttactcactt ttagcgtagt tttatgttga
     2041 atatagctcg ccaaagtttc taggatgggc gttgaaaact gataatcaac agactctaaa
     2101 aagcccgtga caaactccgg caccggcacg cacacccggc gaaaagtttt tggcagtgcc
     2161 ttaattaaat aagtgagttt ttcgcgcaac atgcccggca cgagccattc ggtttgcaca
     2221 ggatttaact gatttaacaa tgctagcgga atagttgcag tgacgccatc caacacatgg
     2281 ccgggttcaa agcggtattt caacggcgtt tcaacgtgat ccagcagaat tttttcagga
     2341 aactgcacgg ccgtaattgc atctgcaccg tggcgcatga ggtcatcgcg cgttaaatac
     2401 agcagttttg gattaagctt ctcggcctca gtacgccatt tttcaaagct tgccgcgtta
     2461 acaatatcgg ccggtatttt tgcatcgtaa aatgaaaata actgatgttc atccaccagc
     2521 acatcttggc gtcgcgcttt atgttccagt tcttccactt ccgcaatcaa gcgttcattc
     2581 gccataaaaa acggcgcgcg cgtatcaaat tcaccattgg cgagggcttc gcgaataaaa
     2641 atctcgcgtg attcaactgg atttatcggc ccataatgca cgcggcgttt tcgaataatg
     2701 gtcaaaccat acagtgacac gcgctcccac gcattgacca tgcccatttt tctatcccag
     2761 cgcgggtctg aataatggct ttccgttaaa ccgcgggcga gcggctcaat ccaatcgggt
     2821 tcaattttgg ccacgcaacg cgcatagagt ttggtggtat cgaccaactc ggcagcaata
     2881 acccattttg gccgcgtttt ctttaaggtt gagcccggcg cgatgtgaaa gcgaatgcca
     2941 cgcgcacccg cgtatgattc actttcacca tctttaaagc caatgttgcc tagcaagccc
     3001 gccaatagcg ctttgtgaat ttgctcgtaa ttcgcttctt tttcattgcg cttaaaatcc
     3061 atctcagaga ctatttcaag caattgcccg tgcaactcac gccattcttt taagcgtaaa
     3121 aatgataaaa aattgctgtg gcattgattc agtaaatctt tgttggattt tttagtgtta
     3181 agggctgcgt caaaccaatc ccacagcttg aggtagctca tgaaatctga accttcgcct
     3241 ccaaatttag catgggcttg gtcggccgct tcgcgtttgt ccattggccg ttcacgcggg
     3301 tcttgaatgc ttaacacgct tgcgatgatt aaaatttctt ttaagcaggc ttcacgtttg
     3361 gcggctaaaa tcatgcggcc cacgcgcggg tcaagcggca atttggcaag ttgtaaacct
     3421 gtttcggtaa tttggcgacg cgcatccacc gcgcctaatt cttgcaacag caaataccca
     3481 tcggcaatca gccgcgtgct gggcgcttca ataaaaggga attcagccac atcgcccaaa
     3541 cgcaatgccg ccatgcgtaa aattacgcta gccagtgaac tacgcaaaat ttccggctcg
     3601 gtaaactctg ggcgaccgtt aaaatcttgc tctgaatata accgtacaca aataccgcta
     3661 gacacccgcc cacagcgccc tgccctttgt ttggcagctg cttggctgat tttttcaatt
     3721 tgcagctgct ccactttggc acgtgagcta tagcgattca cccgagccaa acccgcgtca
     3781 atcacatatt taatgcccgg cacagtgagc gaggtttcag ccacgttagt ggccagcaca
     3841 atgcggcgca ggctgtggct tttaaaaatc ttttgctggt cttcaatgct caaacgcgca
     3901 aacaaaggca gcacttcaat gtgcttgagc ttggctgaac ggccctgata cttgcgcaaa
     3961 tggtcagcca catcacgaat ttcgcgttca cccggcaaaa ataccaaaat atcgccatcg
     4021 ccttgccgta acaaatcatc tgccgcgtcc atgatagctt cttcaatggc ttcttcttcg
     4081 tcatcctctt caagccagcg tgcttcggtt ttagcttttc gggcgatgcc aaaaatcgtg
     4141 tcatcgtcta aatcaaattg tgcattgtca gcctcagcac tttcttttgc acgaaaaccc
     4201 gcagcgccca atggccgata acgaatttcc accggaaaag tgcgaccaga aacttcaatc
     4261 accggtgcgc cattaaaatg gcttgaaaaa cggtctgcat caatggtggc agaagtaaca
     4321 atgattttca aatcgggtcg ttttggtaaa agttgcttta aatagcccag caaaaagtca
     4381 atgtttaggc tgcgctcgtg cgcttcgtca ataataatcg tgtcatacgc gttaagaaac
     4441 ttatcgccct gtgtctcggc cagcagaata ccgtccgtca tcagctttac gtaggtcgat
     4501 tcagaaagtt tgtcgttaaa cctgactttg tagcccacca cttcgcctaa tggagagtgg
     4561 agctcttgcg cgatgcgtga agctaccgag cgcgcagcaa tgcggcgcgg ctgggtgtgg
     4621 ccgattaagc cagaaacacc acggccaagc tccaaacaaa ttttgggaat ttgcgtagtt
     4681 ttacccgagc ccgtctcgcc gcaaataatc accacctgat gtttggcaat agctgcggca
     4741 atttcgtctt ttttgccgct aacgggcagt tctaaagggt aatcaggctt aggcaaatta
     4801 gcgaggcgcg ctgcatattt ggcttgtgat gcgcgcattt tttgcgccac ttcccccagc
     4861 aaacgctgcg ctttaccatt agcttgggca tcgcctattt tttgcaaatc cgccacatcg
     4921 cgcaattttc gcctaagcgc atgacggtct accagcatac agctggctag cgcattgatt
     4981 aaatttggaa tatttgcttt tggctgattc atgagctgcg cattttacca tgcgtaataa
     5041 ggattcatgc ctgaaaatac tgtattaaaa tgtgcaaaat ttcgatatgc ttattcgttt
     5101 tttcggccca aactttagag agctccactt tgcttatccc taacaatctg ccatttgagc
     5161 gcaaattgat gcgcgccgtg ctcaagcaac acgcaggtat atttttacta ttactctgca
     5221 tgagcctgat atttgctggc ataggtgcag ccgtcattta ttttaaagcg gctgaagatg
     5281 gcgggcttta tatttttggc ggcttatttt tgctggccgg cattagcatc tttattttca
     5341 atttgattag caattacagt agcgtacagt attactatga aaaaggccta ctcaagcaat
     5401 atgggcgtga agtaaacgcc accattacag ataaacgcgt ggatgatgca atcgtaaaaa
     5461 acagccccaa cagcagtgat gcagacacca cgaccgaaag agactgcatc atcgcttata
     5521 gctatgtgtt tggcggcaaa aaatatgacg ctgaaagttt tcttgattcg caagcgctgt
     5581 ttgaccaact ggaaattggt caacgcattc ccattttagt gttaccgcaa cagccagaaa
     5641 caacttaccc cagaattaaa aaactcaaag cgatgctaaa aatgaatttg caacaagcta
     5701 aaaaatctgt cgaaaatggc actgaaaaca ttcaagtgag ccagggtttg atggaagaag
     5761 aatagacgtt ttacagccaa aaaattgggc taaaaaaata ccaacagttg attaaaaaat
     5821 aggcaaatta cctgccattt taaacgcaac cttttttgat gctctgcgag ccttataaat
     5881 aaaggctcgc agagcattta ttttttctcg tacgctttaa tgccgtaaaa acatctttaa
     5941 ataagcgtga tttttactct tgtctgacgc gcaaaaaact ggcaaatttt ggtttgccat
     6001 gttttgtttt atcgcgataa gtgtaggtga ctacgctacc aactttgggt gggttttcac
     6061 gctgtgcgtc ggtaaagccc gtgccgagtt taaagcgaac accatctggc gtttctacca
     6121 ccagcgcacc taatttgccc tgatattttc cgcgcccggc agtgtgcgcc accaccgttg
     6181 cttcggcatc aagttctagc ttgagcttta acagcacatc actgcggcct gtcacgtaag
     6241 gggcatcggc gcggtgcagc atgaggcctt caccacctgt tgctaccact tgcttgagtt
     6301 tttgctcgag tgcggcttca tttttcaccc gaaactgcgt gactgctttt aaatgcggca
     6361 cattggcaac ttttacaata tcattgatac gctgaacgcg cgcggcaaac gtgccgggcg
     6421 catcaggcaa ctcaaatatc aaatacgaaa tgccgcgcca ttcctcttct accggcacgg
     6481 ctttgcgcac tgccccagaa agcgcatcaa acttgccacg ggccagccat agctcgccat
     6541 ccaatggggt attaggaaat ttttctgtaa accaaatcgg tgccgcaatc acgttgcccg
     6601 cacggcttcg caaggttttg ccatcccaca gcgcgcgtac accgtcgtac ttttcgctga
     6661 ccaaataatg ctcaaccgca atgccacgct ggtaaacctg cgccagtaaa atgtccgcat
     6721 tattttgctg agctagcgcc gcctgcgcaa aacacagcac taaaaacgta ctggacagca
     6781 gcctaaaaaa agattgcaag cgcatttgaa actagcggtt tggcaatgcg aagcgcgact
     6841 gcatggtttc taaattgagt tcgtgcaaaa tcgcctgcat gcgttcgagc gcgtttttac
     6901 gcggttggcc agtgtattta gcaatgatga gttcattttt catggaatgt tcccagccga
     6961 caagctcggt gactgtgacg ttataacccg ccgcttctag ctgcaaacag cgcagcacat
     7021 tggtgatttg gctgccaaac tcgcgcgtat gaatcgggtg gcgccaaatt tcactgagcg
     7081 cagttttacc taaactggcc gatttatgct gacgcatcac ttcagccacc tctgcctgac
     7141 aacaaggcac caacaccaag tattgcgcat gtttttgcag gccaaactga atggcatcat
     7201 cggtagccgt attgcaagcg tgtaaggctg tgacaatatc gacctgcgcc ggaatggccg
     7261 cagaagtgat ggattcttcc accgttaaat gctcaaaatg catacgtgta aagccgcaat
     7321 ttgctgctaa ctggcgcgat ttttcaacca aatcggcacg cgtttcaatg ccaatcactt
     7381 ggccgcgctc attttgtttc atcagcaaat cgtacaaaat aaagcctaaa tacgacttgc
     7441 ccgcgccgtg gtctaccagc gttgggttgg cattgtgcgc caacaccgca ttaaataacg
     7501 gctcaataaa attcaccaag tggtacacct gcttcaactt acgtcggcta tcaaggttta
     7561 acttaccatc acgggtaaga atatgcagcg ccttgagcaa ctcaacggat tgatgcggcc
     7621 tgacatcggc cttaagactt tctggaatgg tttgcataag gcattttaat gcgaataagg
     7681 cattttggcc gaattttttt gcttgaaaat aaccggacat ttttgggcac atcatgacca
     7741 atttgcacgc cgaaatgcac gacaacacgg ttgaaaaacg aacatattgc acgccagcca
     7801 tatccactta ttggcaaatg taaatactcg ttaaccgaac ataatctttg caactaagga
     7861 ttgatgcggc ttggcaagca accgcgtact taacatgatg atttggcatc atttttgctt
     7921 atataaacac aaaagtaact acaacttact aaaaacatta acttaatcct ataggttatc
     7981 aaacgcgatg ccaaataaaa atatcagcaa actgagttta attcatgcaa aaaaccccgt
     8041 attatcgcgc ggctttaccg ttgttgaatt gatgatagct gtggccattg tgggcgtact
     8101 ggctgccatt gcaatccctg cgtatagcgg ctaccaagaa aaagcgagag tgtatcaagc
     8161 cgtcaccgat gttgcagcga tgagcgtcac cattgaaaac tactttcaag aaaatagaat
     8221 ttaccctgaa actctcgcag atgttaagtt agatggcaaa ttagatccgt gggacaagcc
     8281 ttaccgctat ttaaaccttg tgaaaaatgg caacggcggt gccaggcgcg ataaaaattt
     8341 agtgccgctc aattcagact ttgacctcta cagcgaaggt gaagataaaa aaacaaaatt
     8401 accgatttct cagaaagata gcttagacga tatacttcgc gctaatgatg gtaaatttgt
     8461 agatttagca gaaaaatatt aaggctaaat tgacggttta gctttatgca aatttcaggc
     8521 acttttctta acagcaaggt cgcgcaacgc atcgcgctat tgctattaat agcagcggtc
     8581 atccccgccg tgctgattac ttggctcaca taccgcaatg tagaccatct caccaaccag
     8641 cattcacaac agactttaaa agcaactagc caaaattacg ccttgcttgc ctttaacaat
     8701 ttgcttaccg cgcgcaaatt tttgcattac gccacgcaaa gctatcatca ccagcaacat
     8761 catgctgaag aaatttggca gcacgcacaa tcaattacca tgttcaacgc tatagttgaa
     8821 atttcagctg acggcaagcg catcagccag cacggccaat accaagattt atctgaagct
     8881 atcaaaaagc aaagtagcag cattattagc agtgccacca acgataaaac tagattaatc
     8941 gtatttcctg ccaagctagc acagcaaagc gcacatgtga atttggcaat gccgatcttt
     9001 aatgaaggcc aattagaaag tgtattgatt gcagaattaa accctgacta tctttggggc
     9061 ttaaaatcca attacccttc agaccagaaa atttgtgcct atcgggtcac tgataccagt
     9121 aaaaccacac ttttttgctc aactgacgcg ctagatattg cccaccaaac cagccaaaca
     9181 ggcggttggg atttgttttt aaaagctgaa ttacacgatg cgccgtggac atttgaaaca
     9241 acccaattta cacctgccgc aaagagcggc ctatgggcgc ttattggtag caacgcctac
     9301 attggtgttg gcctgttaag cttattgatt gtggggctat taagcctaat tcaaattcgc
     9361 agaaccatga ttccgctaga gcgcttaatc gatagcacac gaaaaatttc gcatggccaa
     9421 tttgagtcgg tggaggtgga tggaaaatct gaattttctg aattagccac cgcatttaac
     9481 agcatgtcgg gcaacatcaa acgccaactt gataccttgc aatccctttc tgaagttgac
     9541 cgaaaaattg tcaccaagtt agatattgac cacttaattg agcaagttat ggcgcgcatt
     9601 ttgcacctca agccagaagt cagcgcgagt atttttagaa ttgacgaaca caatgatgat
     9661 gaaaaaactg aaagtgaaat tcaatgtatt gttgatattg cacaagtagc aaatgcttca
     9721 acattgcgta aaaccattac ttcacaagag gcgtcgtatg ttaaaactct caacaacggt
     9781 gtgattttgc cttgtgatgc aaaaagccag tttacacatc aacaaatgat ggcgtccctt
     9841 ggggctaaca ccatttggct attgccaata ttttggcaag gcgaaatgtg tgcattttta
     9901 gtcatcggca gccagcaaaa gttagatgaa catgacagcg acttgcatga gattcgcgac
     9961 cttgccagcc gtattggcat tgtgatttct gcacaaaaac gtgaagcgca attacttttg
    10021 caagcgcact atgacatttt aacgggtttg ccaaaccgta ttttattaga agaccgcatt
    10081 gggcaagcca ttgagcacag cgaccgcaca aaactaccca tgtgggtggt gtttttagac
    10141 ttagaccgat ttaagtttat taacgatagt atgggccaca acattggcga ccaattgctc
    10201 gtgcaggtcg cgcatcgcat gcaagccgcc gtacgtgaag ttgataccgt agcccgtttt
    10261 ggtggcgatg aatttattat cgttttaccc ggccaaactg atgaaacgct gatgatgggt
    10321 gtgctggaac gaattattca catggtttgc cagcccatgc aaatcaacaa ccaagaaatt
    10381 attattacct gcagcgttgg cattgccgtt taccctaatg atggcgcaaa ggctgaaact
    10441 ttaattcagc atgccgacat tgccatgtac cgcgcaaaag aattgggcaa gaataatttt
    10501 caatttttca ctgaatccat gaatgaaaaa gtagctgaac gcatgtatgt ggagaattta
    10561 ttacgccatg cgcttgccaa aaatgagttg gaagtttatt accagcctaa agttgattta
    10621 gaaaccatgc aaatggttgg catggaggcg ctaattcgct ggaacagccc agaattaggc
    10681 tttgtttcac ccatcagatt tattacatta gcagaagaat cagggttgat tgtgccgatt
    10741 ggcgagtggg tgatgcggtc cgcttgccag caaacagtgg cctggcaaaa tgccggttat
    10801 gcgcccttat tgatgtcagt caatttatct gcgcgacaat tcaagcaaag caatttagtt
    10861 cagcgcattc agaatatctt aaaagaaacc ggtttagaag ctcaatattt agaacttgag
    10921 ctgacagaaa gcatggtgat gactgaagtg gaggcttcat tacaattgct gcatgaaata
    10981 aaaaccttag gtattcaaat ttcagtggat gattttggca cgggttactc aagcctctct
    11041 tacttaaaag atttaccggt gaataccctg aaaattgata aatcattcac cgatgacatt
    11101 atcaaccaca ctgacaaagc gcccattgtg caggcagtca tttcactggc aaaaagcctc
    11161 aatcttaaaa ttgtggccga aggcgtggaa tcttacgagc aagtcttgta tctgcaacaa
    11221 catgcctgcg accaaatgca gggctattat tttagccgcc caaaaccggc cgttgaaatt
    11281 gaaaagctat tgattgaagc gcaagcgact gcacccaaat tagcgcatgc gtagttaata
    11341 ctttttgtaa aaaattcaaa aaagctacca actcgtcatt ccagcgcagg ctggaatcta
    11401 ggcaggcaac atgccaacga atatttgaat aaaataatag tcgttaaata ttttatagtt
    11461 acatttaaaa tctataggtt atcaattaaa tatgcaacat ctctctacgc cagtcaatca
    11521 caggtgattg gaccttgact ggattccagc ctacgctgga atgacgtggt tttgatgttt
    11581 ttatttaaaa taagctgctc ttttacttcg tgaaaaagtc tcaaaattca aaaaactttt
    11641 catggtttgt cgtcaaacat atttccactg cgttttgttg agtaagggcg tagatttaag
    11701 cacccgcgta aacttaacaa aggaaatcaa catgaaaaaa acaatcatac ttactgccac
    11761 cctcgcggcc cttaccacct cttttagtgc tctggccgat agagaccatc gcttagacca
    11821 gcgcggtgac cgaattgaaa atagattaga ccaacgtggc gaccgcattg aagaccgttt
    11881 agacagacgc ggtgaccgca ttgaacaccg ccatgataaa agagcagata aagcccgtgc
    11941 gcacggccat gataagcgtg cagatcatct tgaacgtaaa ggtgaccgca tcgaccaaca
    12001 acttgacgac cgcggcgacc gaattgatga ccgattagat agaaaaggtg agcgtattaa
    12061 ccgtcgccta gacagacgcg gcgcacatca cgctaaaaag taaacaacaa tattttggtt
    12121 ataattttaa aggtaatata gcgtattcaa cccgttgagg catggtcgat cacttaatgc
    12181 aacattttct tgctgaaatt gaaggccgtg cctttcgtat cgcacagatt gcgactggta
    12241 atcgtgaaga tgcgttggat attgtgcaag atgccatgat taaattggtg cagaaataca
    12301 gtacgcatgc aaaagatgaa tggaaaccac tgttttacag cattttgcaa acgcgcatac
    12361 ttgattggca tcggcgccaa aatgtgcgca accgttttag aagctggttt cattgggatg
    12421 aagatgaaga cactgaagat tttcttgagc aacaacccgc aaacaatgca agcgcacccg
    12481 atctacaact gcaagatgat cagtttatgg acaatctcaa taccgcattg agtaagctac
    12541 cgcttcgtca gcagcaagta tttttaatgc gggtgtggga aggcatggat attgccgaaa
    12601 ccgctgccgc catgcaatgc tcgcaaagta gcgtcaaaac acattatgca cgcgccttag
    12661 aaaaactgcg cgcacagtta gaacaccatc agtaaccggg tgaacatcat gaataatcat
    12721 caatttgaag ataaagtaaa agcaagccta acgcgtagcg tagaagcaat tgatgccgat
    12781 acacggcaac gcttagcaga tattcgccgg cagtcactca atactgcaag tgaaaaagta
    12841 tcacctgcaa aatggctaac gctcgatttt tggcgtggca atcatctaat gccggcggcc
    12901 tcactggcgt tttgtgcact cattgcggta tttttggtga gttcgcctca aactcaaaat
    12961 cctaccaatg tgattccatc gatggataat cagaatcaag tcgccatttt agagttgttg
    13021 aacaacccag atgagttaga agtgctgagt gaccctgatt tttatctatg ggcagatgaa
    13081 atgttaatgg atgagaccga taatgctgtg taggtttttg ctggcaacct ctttcctgtg
    13141 cttgaccgcc aaccttcaag cgcaagaacc tgcaattccg cttgatttaa tcgagttgct
    13201 tggcgaattt gatgccgaag atactgattc aatcaaagaa gccgttacac aagttggcac
    13261 taatccccaa aaaaacaaac caagcgtcaa acaagttggc caagcgaaaa caacgtctgc
    13321 cgaggtgaga aaatgatttt tttagtaaaa aatgacccat ttgataggtt gtcacatgca
    13381 taaacgattt aattcccgat taaaatttag cgcaatcaca ctgagtttag tcatcagttt
    13441 gatgtcattg accgcgcggg cacattcaga cgatgcagaa ggtgtcgctt gggaaaacct
    13501 aagtgcccag caacaagaaa ccttaagtgc tataaaagaa aattggggca cgttgccggc
    13561 tgaacgccaa cggcgcttaa gcaaaggtgc tgaaaaatgg ggtgcgatgg acccggaaca
    13621 acgtgcgcat attcaagaaa gactgcacca ttatcaaaat ctttcacctg aagataaaga
    13681 agtggtgaaa gagcaaatgc gcgcgtttaa aaaactgcct tctgatgaac gcaaggcatt
    13741 gcgcgaacgc tggaaaaacc taccgcctga acaaaaagaa atggttcgtg aacgctacaa
    13801 agacatgaac catgatgaac gcaaagcgat gcgtgaagct tataaacaaa aaaaacggga
    13861 atggcgcaaa catcgcgaac acgactgatt tgaatgaatt ttattgtgtt agtttttaaa
    13921 tgtttaaacg agtcggaaac acgctgacac tctacaacgt taatttatca aaaataacgt
    13981 cgctcaaaaa tgttaaaatg ccgtattcac gcattttaac aatcaagccg caccactaaa
    14041 cgtagcacta cgtatactcg cggccaattt ttaaggtttc aatatggcca ttcaatggta
    14101 tccgggacac atgacccaag cccgcaaaaa ggcggaagaa accatggaat tcaccgatgt
    14161 ggtgattgaa gtgcttgacg cgcgcgtgcc agtagccagc cataacccaa tgattgatga
    14221 aatgcggttg tttcgtcagc gcccacagct aaaaattcta aacaaatctg atttagctga
    14281 ccccaaagca acgcaagcct ggctgaacta ctataacaag caacccaata ccaaagcggt
    14341 tgccctttca tgtaaaaagg caggtgaagc caataaaatc cctaaactct gcttggggct
    14401 ggcaccgcat cgcggcacgc atttaaaacc cttgcgcatg ctgattatgg gcattcccaa
    14461 tgtgggtaaa tcaaccttga tgaacgccct gcttaatcgc cgtgtggcga aagtgggcga
    14521 cgaaccggca gtgaccaaaa gccagcaacg atttgattta agtgacacca tgagcatcac
    14581 cgacacgcct ggcatgatgt ggccaaaaat tcaatatgaa tccgatggtt acatgctggc
    14641 cgccagccac gctattggcc gcaacgcggt gattgatgaa gatgtggcat tatttttggc
    14701 caataatttg ttgctcacgt acccagcgct actgaacgcg cgctacaagc tcgacaccat
    14761 gaagcatgaa ggtgccatgt tggatgtact aaaaatggat ggagtggatt tattggaagc
    14821 cattgccaga aggcgctctt acaaacgtca tgacggcctg tatgacatgg aaaaaacggc
    14881 tgtggctttt ttgacagatt accgcagtgg cgcgattggc cgtgtaacat tagaaacgcc
    14941 tgaaagcaga agcgcaatga tgcaaagcac tcaagccgtc aatgaggcag aaacaaccga
    15001 aaatgaagcg cctgacgaaa caaatgccga ctaaacagca ctaaaatgtg cgaaattttt
    15061 gaaaattagc ggcactgccc aagggcaatt tcaccttgcg aacaagtgcg cacttggcct
    15121 ttttttaggc cacccgtaaa aactccccag ccagatttct cgttatcttt tgtttgcagt
    15181 tgaggctcta cttttggcgc cacggcttct ggctgtttca cgtgttcctc tttaggtggc
    15241 aattcacttg ttttagcggc ttctgttgcc tcagcttttg tttcagtggc tgacaacgtc
    15301 acttcgggta atttattttg ctcaaccacg ggtgccgatt cagttttggc ctgactttgc
    15361 gcctctaact tcagttgctc agcaaacact tttctggcta acttttctga ttcctcatca
    15421 gcgatttgcg ataccgcgcg gatggttttt tcaggtttga ttacaggcgc aaccacttcc
    15481 tcagatttct gctgaggtgc tggtaattct tctatcgttt catttggcaa taatccataa
    15541 gggtccaaca ccgttggttg tttaacttca ggttgcttca ccgctacagg tgctggtggt
    15601 tctattgttt cagcgcttgt catggtaatg gtcggttcat tcggtatggc cagcgtttgc
    15661 actaacgcat ataaaccaac caaaatcagt atgccactca aggcataggg ccaatgaacc
    15721 cctttgaatt cagccaatac ttcaataggc ggctctgtat tttcatgttt aatatcaact
    15781 attggcgctg gcccttgtag cgcaaatgcc ttatttaacg cggccaggcc ttctgtaaaa
    15841 tgcatggcat agagtggcag acctaaatca tgccgctcgt tcaaaatacc tgcataatca
    15901 atgacattgg tttccccctg catcaaatgc gcagtcgccg ccacaaacgc ttgtgtggcc
    15961 aacatttcat ttggctgggc aaaactggca atacgttgcg ctaatttgat ggcgtcgccc
    16021 agcacctgcg gttggtcatg aatgtcgtta acgatttgca cctgcccaga atgcagcgca
    16081 aagcccacca atactggcgc agtcgtccga atgttatatt gatggacgat attttgcatg
    16141 ttggcggcaa tttgcaacac ttcctctggt gcgcccaaag tggtcacgat aacgccctca
    16201 accgtatctg tcacaatacg tgtgtgtgtt tgcggcactt taagggccac ttttaccagt
    16261 gcattaaatt tacttttgag gtcaatttgt tcagcatcag ttttatctga aaaatcaacc
    16321 agttctaaat aaacaacgtt gccagaagcc gttttgtgaa tactgtcatt catcataagt
    16381 ttgaagtttt gctcgcaatg cgttttttac tagcgcatca ataggtgaaa tatatagggt
    16441 gattttaaac gggttgccgg tctgatttgt taaattttgt taatacgcag gtaagttgcc
    16501 gcagttttaa atgcatcaaa ttgtgcatgg cgctattttt taaccaactg cttgttaaac
    16561 gcaatatcta acgcgctcac gcgcttaggg gtttgcgggc cataggcatt tacaaaggcc
    16621 acaaaatcgt ccaaaggcat aggttcacat aaacgggtgg cctcacctgc cacgcacaaa
    16681 ctaaataatg cgccttggtg caaggtcatt gaatattgcg tgttgccgct tctgtttttt
    16741 agccgatcaa tggcggcact ggctttggtt gatcgcgttg aacgcatcgc accctgcttt
    16801 atttttttat atcaagacca caattatggc atgcttttag ccatgcatct cgcataaata
    16861 caagtcaaaa aaatcgcaaa aaacatgcac aattctcccg agaaaaaaat aatgctctgg
    16921 aagcctttat ttataaggct tccagagcat cgaaaaaggt tgtgatataa aaagcagcat
    16981 tttgcattaa aatttaacaa aaaaaacatt aacaaataac ctgacctttt acttaagtgc
    17041 ttaagttttt gatagctagt tttaacttaa aacgctaaaa tcgcgtgttg aaattcacgc
    17101 tcgaaatccc tttttaagat cattcccaaa caaatcacgc atgctcaact ctcttaactc
    17161 gccgcaacgc gaggctgtta aatatctgga tggtcctttg ctggtgctgg ctggcgctgg
    17221 cagtggcaaa acacgcgtaa ttacgcaaaa aatcagctat ttgattgacg aagcaggcta
    17281 cagccctaaa gaaatcgcag cgattacgtt taccaataaa gccgcgcttg aaatgcgcga
    17341 gcgcgtgagt aaactgatgc aaggcaaaag tattaaaggc ctaaccattg ccacgtttca
    17401 ctcgctcggg ctgcaaatgc tgcgcgctga ggctacgcta cttggttaca agccacagtt
    17461 ttcaatttta gattcgtctg acagttttaa gattttaagc gacgtattgg caaccaccga
    17521 taagcaattg ctacgcaaaa cacaatggca gatttctagc tggaaaaatg cttttatcaa
    17581 cccggaccaa gccaaagcca ccgccgatga agaattgacg cacgccgccg ccaaagtgta
    17641 tcaaatctac cagcaaaccc taaaagctta ccaagccgtt gattttgatg atttaattaa
    17701 attacccgtt gagctttttg aacagcatga tgaggcttta aataaatggc agcgcaaact
    17761 gcaatattta ttgattgatg aataccaaga caccaatgcc tgccaataca agctggtaaa
    17821 aatgcttacc ggtgtgcgcg gccagtttac tgcggtgggt gatgatgatc aggcaattta
    17881 cggctggcgc ggtgcggatg tagaaaactt acgccagctg acagaagatt tcagcaagct
    17941 caaggtgatt aaactcgagc agaattaccg ctcaaccgtg cgtattttac gtgctgctaa
    18001 ccaagtgatt agcaataatc ccaagttgtt tgagaaaaaa ctttggagtg acttaggcac
    18061 aggtgacatg ctgcaagtca ccgccacgat gagtgaagag cacgaagctg aaagcgtagt
    18121 gatgaaacta caggcacaca aatttgaaca tcgcaccaag tttatggatt acgcgatttt
    18181 gtaccgtggc aatcaccaag cgcgcatttt tgagcagcac ttacgcaatc aaaaaattcc
    18241 ttataccatt tctggcggcc agtctttttt tgataaaact gaaatcaaag acttaatcag
    18301 ctatttgcgc ctaattgcca acgaagatga cgacccggcg tttattcgcg cagccaccac
    18361 acccaaaaaa ggcatcggca acaccacgct tgagcgactg ggcgaatatg cgagtgcgca
    18421 taaaatttca ctcttttccg ccgcctttga aggagacttt caaagcgaga ttggccacaa
    18481 acagttagat gatttgctca atttttgtac ttacattcaa aaaatacaag cccgtgcagt
    18541 gcgtgatccg gcaggggacg tactgaacga cctgctcacc agtattcagt atgaagcctt
    18601 tttgtacgac actgacgaac cgcgtgccgc cgaggcgaaa tgggccaatg tgatggattt
    18661 tattggctgg ttgaccaaaa aaggtgaagc aaatactgaa tttggcaatg aagcggaggg
    18721 caaaaatcta cttgaactca cgcaaatggt gtcgctcatg agcatgctag aaggccgtga
    18781 aaacggcgag cccgacgctg tgaaactctc aacactgcac gccgccaaag gcctggagtt
    18841 tggtcatgta ttcttaattg gtgttgaaga aggcatttta ccgcatcgcg aaagcgtgga
    18901 tgccgccacc gctgacccca gccgtattga agaagaacgc cgcctgatgt atgtgggaat
    18961 tacgcgtgcg caaaaatcat tgaatatttc atggtgcaaa aagcgaaaac gtgcaggtga
    19021 aatggaaatg tgtgaaccca gccgttttat tgccgaaatc cccaaagatg atgtgcgcca
    19081 ttttggcaac ccattcaata aagacccgga agccagaaaa gactttggta aatctaaaat
    19141 ggccaacatt cgcgccatgc tcggcaaagg ctaaatgact gttttttttg aattgacaat
    19201 ttattactgg acaatttaat actggacaat ttaatgctag acgttagaaa aattacgcaa
    19261 cgcattatat tcgcttatat aatagttttt ttaattcgtc tattctagaa atagcctatg
    19321 aatattggcc gcaagctcat gctcattgtt gcctccagcg ttgcgctggt gacgattcca
    19381 gccgcaacgg gtatctattt ttacgtagcg cataacttgc tcgcgagcga agcagcaaca
    19441 caagttgctg aaaccagcac actggtgatg gccaatacac aaaaagtgca aagctatgaa
    19501 ataggcctgc agtctttatc taaaaccctc acaaacacat tatccgcacc gcctttagcg
    19561 ggtgaagaag ccgcttttga tgccattata cagcgccatc cagatggtgc ttggcgcaac
    19621 aaacgcgcaa catttaacgg taaagaagaa tcagggctat ttttaccgcc tgatgcatcc
    19681 atggatattc accaaaaaag gctgcacttt cgtaccaaac aggtcttaga tgcgtttggt
    19741 agcagcatca ccacgcccac aggaaatatt tgggtgctga cccatgataa aactgaaatc
    19801 atatttgacc atttataccc tgattttacg atgctgatgg cggctgatac cgactatact
    19861 aaaacaccgt ggatgacgtt aggcgacccc gctatcaacc ctaaacgcga agtacgctgg
    19921 acatccccca tccaagaccc ggtaaccaaa agctggatta tcagcgcaat attaccgctt
    19981 gacatcaacg gccgttggat tggcaccatc ggccgcgaca ttggcctcaa tgttgactcg
    20041 ctttcaattt ttcaaaaaca tgaacacttt ccaggtgaac tacgtttttt acttgatgcc
    20101 gagcgccatt ttattgaggc tggcccttgg caaaccttac tccagacgaa tccgcaagct
    20161 attcaatcac atttagctaa tgaaccagcg ctggctgaac ttttgaatgc cagtaccgct
    20221 acagccagtg cttatgataa aaaagtcact atacaaggcc gccaatactt agccaccagc
    20281 atggtgttgc ctgtggtggg ttggcattac tttaggcttg ttccgcaaga tgaaatactc
    20341 aaaccgatgc aacaactgtt ttacacgctc accgcgctca tactggcgat tggcttactc
    20401 atcggcagcc tcattgaaat tgccgtacag cggcagatta tttcacgcat caaagtgctg
    20461 gcaagcacct tacgccgcta tagccttggc gatttgagcg cacgaacaaa cttaaagggc
    20521 aatgatgaaa ttgcacaagt gtcgcatgag tttgatgaaa tggccggcca gattaaatcc
    20581 acccttgacg cactcccaga tattcttttt gatctagact taaacggcca atattatgcc
    20641 gtacacacac ccgatgaaaa attactgact gcgccagtga aagaactcat tggcagaact
    20701 gtaccgcaaa tgctgccaca gcacgcggcc gctattgtga tggctgcact caaagaagcc
    20761 aatcaaaagg gttggtcgcg cggcatgcaa tacttaagaa aaacgcccat aggcacattc
    20821 tggtttgagt tatctgtggc caaaaaaacc aattctgatt ttgaccaccc acgctttatt
    20881 gtgctttcgc gtgacattac agagcgaaaa caatttgaaa gccgcttgag tgaaagtgag
    20941 ttccgctgga agttcgctgt agaaggcacg ggcgatggca tgtgggactg gaatattcaa
    21001 accaacgagg tgcaatattc aacccgctgg aaaagtattt tggggtttgc tgaggaagaa
    21061 attgagccca cacgcgaggc atggaaaaaa ctcgtgcata ctgaagacat tgctgaagtc
    21121 atgcaggcga tcagcgaata tctcactggc agaaccacga cttacaccat tgaatatcgc
    21181 atgcaaagta aaaacgggca atacttgtgg atattagaca aaggggtcat tgttaaccga
    21241 gatgccattg gcaaaccttt gcgcatgata ggcacgcata cggatattac tgagcgtaaa
    21301 accatcgaac acgaccttcg tatttcagca atcgcgtttg acgcacaaga aagcatgatg
    21361 gtgtcggacg ttaacaaagt gatcttacgc gtaaacaaag cgttcacggc cattacgggt
    21421 tacgctgcag aagaggtcat cggtcacacc gcattaccat tgcgttcaaa tcaacacgag
    21481 gccagttttt ataaggctat ttgggagggt gttgacgcga atggcgcatg gaaaggcgaa
    21541 atatacagca tgcgtaaaaa tggtgacgtt taccctggcc tattatcgat tacagcggta
    21601 aaaaataacc agggcgccat caccaactat gtcaccacgc tggttgatat caccaaagaa
    21661 aaaaccactg cccaagaaat tcaattctta gcgttttacg acccactcac gggccttgct
    21721 aaccgacgct tactgctcga tagactaagc cacgcactcg ttgcccataa ccgtagtggc
    21781 cgcgatggcg cattgatctt tcttgacctt gaccacttta aatcgctgaa cgacacgcta
    21841 ggccacgatg tcggcgacat gttattaaaa gaagttgcaa accgcctgac cgcttgcgtg
    21901 cgtgaagacg acaccgtagc gcgattaggc ggggatgaat atgtggtttt actcgaaaac
    21961 ttaagtgaaa aagccataga agctgccaca caagttgaag caatcggcac aaaaattctt
    22021 gccgcgctta acaagcctta ccaactaggc acacacgaac accacagcac acccagtatt
    22081 ggcgcagcac tctttagtga ccatacagaa acacaggaag agttgctcaa gcatgctgac
    22141 attgcgatgt atcaggctaa aaaagctggt cgcaacacac tacgctttta tgacccacaa
    22201 atgcagcaag ccattcatgc ccgcgtgagc ctagaacgtg agcttagaaa agtaatagaa
    22261 aaacaacagc ttagacttta ttttcagatt caggtcaata gtgatggcga gcctattggc
    22321 gtggaaggct tggtacgctg gttgcatcat gaacgtgggc tcattccgcc cttgcacttt
    22381 attccgcttg ctgaagaaac cggcctcatt ttgccaatcg gccagtgggt gttaaacgct
    22441 gcctgcgcgc aacttaaact ttggcaaaat atggcattaa cgcaagattt aatcttatct
    22501 gtgaacgtga gcgctaaaca gttccgtcaa aatgacttta ccacgcaagt caaaacagcg
    22561 gtcgagcagc atggcattga ccccaccaaa ctcaaattgg aactcactga aagtatctta
    22621 ctggaagatt ttgaaggcac cattgccacc atgaacgcgc tgaaagagat tggcgtacta
    22681 ttctctctcg atgattttgg tacaggctat tcatcactgc aatatctcaa acggctgccc
    22741 ttaagccagc tgaagattga tcaatcattt gtacgtgaca tttttacaga taacagtgac
    22801 aaggccatcg tcagcaccat tatcgccatg gcgaatagtc tcgatttgga agtgattgcc
    22861 gaaggcgttg aaacaaaaga acaacagcaa ctgctcatgg acatcggctg caaagcttat
    22921 caagggtttt tatacggcaa acctgtagac atccagcaat ttgaggcggc acttaaaaac
    22981 acattagttg acccgcgctt ggtataatgc gcgcttaaat cttgagaaat gcaattgatt
    23041 gcgtgaatac gctgtattta aagatcattg ctaatgacta ttactcatga ccaatgcttt
    23101 agtcgaatcc attgcactcc tgtaaagcca accagaaaac caacatgccc tacattactc
    23161 tagataacgc ctccttagcc tttggccacc acgccctact cgaccacgcc gcatttcagc
    23221 tagatgctgg agagcgcgtg ggcttgattg gtcgcaatgg ggcaggtaaa tcaagcttat
    23281 taaaagccat tgccggcacc attaaactag atgaaggcac cgtgtggcgt gcccccaatg
    23341 cgcgtgttgt ctatgtgccg caagaacctg agcttgacac cacgcacact gtgtttgaag
    23401 cggtagcaga aggattgggt accctgcaac aaaccatcat tgactaccat caagtcaccc
    23461 atgacatggg catgcctgac gcagatattg aagcactgat gaccaacatg cagcacttac
    23521 agcatgaaat ggatgcgcaa aacggctggg cagcgcaatc gcgcgttgaa accgtattga
    23581 gtcgcttaaa tttggatgca gatgcgctga tttctacgct ttctggcggt tggcgcaaac
    23641 gtgtggcgct gggtcgtgcg ctggtcgctg aaccagaagt attattgctg gacgaaccca
    23701 ccaaccactt ggatttagaa gccattgagt ggcttgaaaa tttactgttg ggctttaacg
    23761 gtagtgtgct gtttatcacg catgaccggc gttttttgga tagtctggcc acgcgcatca
    23821 ccgagctaga ccgtggcaaa ctcaccgact ttatcggcaa ctttacgcaa tatcaggcaa
    23881 aaaaagaaga actcatcgcg gtggaagaga cccacgccgc taaatttgat aaagtgctgg
    23941 cgcaagaaga agtgtggata cgccaaggca taaaagcgcg ccgcacccgc aatgaaggcc
    24001 gcgtacgtcg acttgaagcc ctgcgccttg agcgcgccgc gcgccgcgag cgccaaggca
    24061 atgtaaagct caatattgat gcgggtgaac gcagtggcaa gctagtggcg gaactcgaca
    24121 atgtttcaaa atcatacggc gacagagtcc ttattaagaa cttcagtacg cggattttac
    24181 gcggcgataa aattggcttt cttggcccca acggcattgg caaaaccacc ttactcaaac
    24241 tcattttagg tgagatagaa cctgatagcg gtagtatcga acgcggcaca aaaattaacg
    24301 tagcctattt tgaccaaatg cgcgaacagc tcaatgaaga ggccacatta gccgacacca
    24361 ttagcccggg ctctgatttt gtcgaaatcg gcaacgagcg caaacacgtt atcagttatt
    24421 tagaagactt tttattcccg ccacaacgct cgcgctctcc cgtcaaatcg ctttctggtg
    24481 gtgagcgcaa tcgcctgcta cttgcccgcc tattcgcccg ccctgcgaat gttttggtgt
    24541 tggatgaacc caccaacgac ttggacattg acacgcttga actcttagaa agcttactgc
    24601 aagaatttac tggcacgctg tttttagtca gccatgatcg cgccttttta gaaaacactg
    24661 tcacgcaagt gattgcgttt gaaggcgatg gcaagctcac cgaatttggt ggcggctatg
    24721 acgactggca acgattcacg caacaacgcc aactaaatca aaaggcagtg gataaaaaag
    24781 cggaggctac ggctaataag acaaaaagcc aagccacaga aacgcctcca aaatcagctt
    24841 caaaactgag ctttaaagaa caaaaagagc tcgatgaaat tccgcttaaa gttgagcaat
    24901 tagaagcaga acaaaccaca atcaacaccc agcttgccga cagcgaactt tataaaacgc
    24961 aagccacttt ggtaaaaaca ttgcaatcca gactcaacag catcgacctc gaacttgaaa
    25021 gcctgctcgc ccagtgggaa aaactagaag ccaaaaaagc gtagttattt tgtaaaaatt
    25081 gcagttaatt tttgcctaat tataggcaat taatctccga ggaaaaatta atgctctgca
    25141 agccttgtaa ataaaggctt ccagagcatc gaaaaaggtt gtgcttgaat ttggtacccc
    25201 cgacaggcat ctaaatgtgc tttgtatggc ttataaatac ttgtcagtaa aatataagct
    25261 aaatttttat acgtattttt atacgttttt cgttccactt accctaataa ctgcattagg
    25321 tataattgct aaaggtggtg cattttatac aaacaaattc caatttgcca atgagtaaca
    25381 tcaaatactt gattgcctca tgttgctgat tattgtattt ttttatcctt gagtgtcccg
    25441 ccagtgtccc atgggggcgg gggtatgttc gcaggttggg ggtggtggtc gacagtgtct
    25501 ttagctacat gcgccctatc cacatcacat ataaaattac gatttatagg tg
//
LOCUS       DAOQSI010000014        22783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_1492_length_22783_cov_5.602209, whole genome shotgun sequence.
ACCESSION   DAOQSI010000014 DAOQSI010000000
VERSION     DAOQSI010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 22783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22783
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1492_length_22783_cov_5.602209"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..1044)
                     /locus_tag="PKL42_02005"
     CDS             complement(160..1044)
                     /locus_tag="PKL42_02005"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNU65873.1"
                     /translation="MQFQLKTMLMAVSSATMMLAATGCATMDMGSSKAKTEATGSAGG
                     GNAVNANATLEKCDKPLGTLAVVEDTNADWYRQLTGQYHLGPTTPVLKLLVQQSNCFV
                     VVDRGAAMALGNGERNLRDSGEMRETSNFKKGQMVAADYAMNPTITFSSNDTGGVGAG
                     LGGWGGGWLGLLAGGLKFADASTMLTLVDNRSSVQVAAAEGSARNTDFSLFGGAFGSR
                     MGAAGGGYTKTPEGKVVVAAFTDSYNNLVKAVKNYKTQTVEGGLGAGGNLAVQAEEPV
                     VKEPVKKTTTTKKKKVKK"
     gene            1446..3662
                     /gene="uvrD"
                     /locus_tag="PKL42_02010"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             1446..3662
                     /gene="uvrD"
                     /locus_tag="PKL42_02010"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HNU65874.1"
                     /translation="MEIEHSPQSPSTLLAGLNNKQLEAVTLPHQSALILAGAGSGKTR
                     VLTARIAWLIQTGQVSPTGLLAVTFTNKAAKEMLTRITASLPINTRGMWVGTFHGLCN
                     RLLRTHHREAGLPQSFQILDIADQLSVIKRLMKLMNVDDEKYPPKQVQNYINGCKDEG
                     LRAHAVEAYDAHSQKMREIFDAYDKQCQRDGVADFAELLLRCYELLERDANIRQHYQS
                     RFKYILVDEFQDTNRLQYLWLKLLAGLGNCMFAVGDDDQSIYGFRGARVGNMRDFEKD
                     FNVQNIVKLEENYRSHSNILDAANAIISHNHNRLGKNLWTSAGAGEPVRVYDAYNDTD
                     EAQFIVDEIKMLHCEGTSLGEIALLYRSNAQSRILEHALFSANLPYRVYGGLRFFERA
                     EIKHALAYMRLIANANDDTALLRVINFPARGIGARSLEQLQEAARAQDCSIWQAAINK
                     VGNGKLGGKGIEGFVALIRQMIDGAYGISLSELTELAINQSGLMAHYENDKEGEDRIE
                     NLKELVTAAVSFTNQDFGNHNNVDGDTEQDLLTQFLSHASLEAGEHQADVGHEALQLM
                     TVHASKGLEFKVVFISGLEEGLCPHEQSLYENAGLEEERRLMYVAVTRARQRLYLSHA
                     QSRMLHGKVRYGIPSRFLDEIPEKLLKRLNSKPVSKPSYSRDYNELPKIMNTQQSSDQ
                     KSAMPWKIGQQVAHAKFGNGVVVSYEGNASDMRVQVNFGREGLKWLAMEFAKLTPI"
     gene            complement(3797..4546)
                     /locus_tag="PKL42_02015"
     CDS             complement(3797..4546)
                     /locus_tag="PKL42_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HNU65875.1"
                     /translation="MTEPQRNNPPESTPLNIRQVPASHAWAWIVSGFQLFKAYPLMWI
                     VLFVIYLAIAVPVSLVPLLGTVFGALLAPIFAAGMMMGCLAIRRHQELEINHLFAGFK
                     QNTGQLVAVGGLYMMALLMVSVIVVTSLDQEIVALIASGQELTPEQAGSLLKPSTFIA
                     LLLMLPVLMAYWFAPLLVRLHQLTAVDAMKISFIAVWRNVLPVLAYILIFGVLTILAI
                     VPLFLGLLVVVPLMITSTYTSYLDIFGIEQN"
     gene            complement(4548..5546)
                     /locus_tag="PKL42_02020"
     CDS             complement(4548..5546)
                     /locus_tag="PKL42_02020"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HNU65876.1"
                     /translation="MSVFTTITQQELSTWLQNYSIGALVELKGISSGITNTNYFVTTQ
                     TNDGVTTKYVLTLFEQNNIEELPYYIDLMSHLAAHGIPCPKPIADLQGVCLRVLNGKP
                     AALVSCLVGQDVQQPNLAQCAEVGRVLAQMHLAGQTFEAQFPQHFQLGNTIHNQRDAD
                     WRRVTAQNVFAKLSEEEQQLLTTELDYQQNFAMNNLPHGVVHGDLFRDNVLFDGDKLG
                     GFIDFYYACHDGLAYDVAIAVNDWCILPDGYFDEEKLATFLAAYQTVRPFNEAECLSW
                     HALLRRAALRFWLSRLYDFHFPAEGELTHAKDPNHFKQILQQRIALQAAAAKAKDV"
     gene            complement(5695..6069)
                     /locus_tag="PKL42_02025"
     CDS             complement(5695..6069)
                     /locus_tag="PKL42_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HNU65877.1"
                     /translation="MRKNFWLIAGLLALVTMPFSVMAEEARSKAPQPTPTPADAVPLE
                     DIPPPVIGKDDNVDEPEITIVKKKGETIEEYRVNGQLYMMKITPAHGVPYYLHKEDQD
                     GEWINYGPVQPVSIPKWTIFRF"
     gene            complement(6143..6871)
                     /locus_tag="PKL42_02030"
     CDS             complement(6143..6871)
                     /locus_tag="PKL42_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNU65878.1"
                     /translation="MSFAKKVPKITDPDVQGLHHTGHESWHAFEIMAEFVDATERLKE
                     ITPAVSIFGSARTPEDHPYYKLTEEIARLLSDAGFAVISGGGPGIMEAANKGAFPGKA
                     PSIGLNIELPHEQQANPYQDVSQNFKHFFMRKVMFVKYASAYVVMPGGFGTLDEVMEA
                     ITLIQTGKTLKMPIILVCEPFWRGLIDWVKTTLVNEKMISPEDLDLIQIIDEPAKIVE
                     AIFKHYETRGFKLSAAEERVQLYL"
     gene            6892..9678
                     /gene="polA"
                     /locus_tag="PKL42_02035"
     CDS             6892..9678
                     /gene="polA"
                     /locus_tag="PKL42_02035"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNU65879.1"
                     /translation="MKTLLLVDGSSYLYRAFHAMPDLRNSRNEPTGAIQGVLNMLRRL
                     HKDYPSDYSACVFDAKGKTFRDDIYPEYKANRASMPDDLRAQIEPLHEAIKAMGWPLI
                     MEDGVEADDVIGALAKQAEREGVRTIISTGDKDITQLVNEHITVVNTMRDAFRKVDDV
                     LDIAGVERKFGLPPSLIIDYLILVGDTADNVPGVEKVGPKTAVKWLTQYGSLENIIAN
                     ADHITGVVGENLRKALPWLPTARELITIRCDVGIQENLNDLAPQLQDKEKLLALFDRF
                     EFKSWKRELDAEGKTPALQANVYKASSDGQKGLSQIPAEIASEVSAYSPNLSRQYETI
                     FSPAQLEIWLEKLSKAVLICFDTETTSLDPLTAKIVGMSFSVEAGSAAYLPLKHDYFD
                     APEQLNFAEALAKVKPILENANIKKVGQNLKYDQHVLANHGIMLNGIAHDTMLQSYVF
                     ESHKTHGMDALSERHLGITPISFESVAGKGAKQVGFNQVTVEVAAEYAAEDADITLQL
                     HEAMHPIIAADAKLLYIYQQIEMPVSEILFTIERNGVLIDANMLNSQSNEIGLKLIAL
                     ENQAYELAGQPFNLASPKQLQEILFDKLGIKPTKKTPSGAPSTDEDVLQELALDYPLP
                     KLLLEYRGLAKLKSTYTDKLPKMINPQTGRVHTSYNQAVAITGRLASSDPNLQNIPVR
                     TAEGRRIREAFIAPPGSHIVSADYSQIELRIMAHLSKDAGMLQAFANNEDIHRHTAAE
                     IFGVERDAVDNEQRRYAKVINFGLIYGMSAFGLAQNLNIERSAAANYIERYFARYPGV
                     REYMDKTRDIAKQKGYVETYFGRRLWVPEISSPNGMRRAGAERAAINAPMQGTAADLI
                     KLAMIAVDIWLRDEKLTTKLIMQVHDELVLEVPENELELVKVKLPELMQNVAKLDVPL
                     LAEVGVGSNWESAH"
     gene            complement(9682..10362)
                     /locus_tag="PKL42_02040"
     CDS             complement(9682..10362)
                     /locus_tag="PKL42_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65880.1"
                     /translation="MVQENRNSGTPSEFALVYEGKTCIKCNYQRQATDDNTPEWACPS
                     CGVVYAKAEAAERERQADEALLNRIERVEFVKTNQPIDVKEVAKIKSDKSIANLIYIL
                     YFGSIFGGVSGLAAIVLAHMHRKDMGKQNWVQSHYAWQISTFWFSLLWGCLGAFLVFA
                     SFLTASAKGLQARSLNEAIGSFFNPFMLIGFAMFVIASLWFWYRVIKGCIVLNRDAPV
                     GDFYDASM"
     gene            complement(10429..10953)
                     /locus_tag="PKL42_02045"
                     /pseudo
     CDS             complement(10429..10953)
                     /locus_tag="PKL42_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030139.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            complement(11686..13179)
                     /locus_tag="PKL42_02050"
     CDS             complement(11686..13179)
                     /locus_tag="PKL42_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNU65881.1"
                     /translation="MKTTAIQFEQYRQLLSVNNRTLITSSIMAVILCYWQREVTDPII
                     LKGWLALILGINLARVVIGFYYRQVPTVDLQEIQKRLKTFRIGIIAASVVWGTTSLLM
                     FPTNNIEQQMLLLYMLTGFSAGSFYSYSIDFISAIAYTLFALIPLLIRFLATGESFPM
                     LMGASGFVFMAFTVLSLRNVNQSQHETIGLRVDAVEKEKEITASEARYRMLLNHSPVG
                     IAHFDGDLNIIYCNQSLADILQSPQDEIINRNILMIKDDAILPTLNKALAGEVATFDG
                     QYRATISEAEGWMELTAAPAFDSAGIVVGGIAIVHDVTAQKQAADEIKQLAFYDPLTK
                     LPNRRLLLDRLDHALTISVRSGKRGALLFIDLDHFKVLNDTLGHDMGDLLLKLVAERL
                     QDCVRDSDTVARIGGDEFVVLLEGLSKEPLEAASQAEIRANKILTALNKPYQLGKHQH
                     HSTPSVGIAVFRDHGNSQVELLKHADIAMYQAKKAGRNLMKMFDYEM"
     gene            13362..13652
                     /locus_tag="PKL42_02055"
     CDS             13362..13652
                     /locus_tag="PKL42_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNU65882.1"
                     /translation="MNKQQAIQNLSNSKVILSQRFGVNRLTLFGSTARNTATAISDVD
                     ILMGFDGVADSRRYFGVQFYLEDLLHCPVDLVTEKALRPELRPFIEREAIDV"
     gene            13750..13983
                     /locus_tag="PKL42_02060"
     CDS             13750..13983
                     /locus_tag="PKL42_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNU65883.1"
                     /translation="MNSGITYDATLRSLELIGEAATHIPPEIREQYSNIPWRMMIATR
                     NTLIHGYLGIDNDTLWSIIQTDVPEVLTELQKI"
     gene            13996..14736
                     /locus_tag="PKL42_02065"
     CDS             13996..14736
                     /locus_tag="PKL42_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNU65884.1"
                     /translation="MQNIGIYIIGDEILSGKRQDAHLSKVISLLSTRGLQLSWANYLG
                     DIPAQITSSLKASMARGDIVFSFGGIGATPDDYTRQCAADAASVPIERHAGAVAEIEA
                     QYGEGAYPKRVLMADFPQGCELIPNPINRVAGFSIHQHYFVPGFPQMAHPMVEWVLET
                     YYQPLFHQQDYVEASILVMDAGESKLIDLMNHILHQYPTLKLFSLPKLDDRRTTEIGM
                     KGAEKPVKAAMQDIKEGVSALGFPWSDL"
     gene            14768..15025
                     /locus_tag="PKL42_02070"
     CDS             14768..15025
                     /locus_tag="PKL42_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNU65885.1"
                     /translation="MECRANCGACCTAPSINSPIPGMPKGKPAGVRCIQLDELNRCMI
                     FGKPERPPFCASLQPNIEMCGDTREQAMIWLSKLEVLTKPN"
     gene            complement(15022..16311)
                     /locus_tag="PKL42_02075"
     CDS             complement(15022..16311)
                     /locus_tag="PKL42_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNU65886.1"
                     /translation="MKILQSSSPSTTHIAFASFIGTAIEFYDFYIYAMASALVIGQVF
                     FPASDPTAQALNAFLTFGIAFIARPFGAILFGHFGDRIGRKATLAASLLVMGISTLLI
                     GCLPSYESIGTWAAILLCLLRFGQGIGLGGEWGGAALLATENAPANKRGWFGMFPQLG
                     PSVGFLLATLSFLVLTLYLSDEAFKSWGWRIPFFASALLVLVGLYVRFKLAETPAFAA
                     VLAQHHTHKAPIKSLLSTHFRPVLLGALAMTVCYNLFYTATVFCLSYGTLSLNIARAD
                     FLQMLCVAVLFMAAITPISAGLSDKFGRKPVLLIGCLLAILAGFSLAPLMASGSLWQI
                     TLFLSIALLLMGATFAPMGAFLPEQFPVAVRYTGAGLSYNLGGILGASTAPFISQLLV
                     DKGGLVWVGYYVSAMALVSLVAIILMNETQQRNFNEA"
     gene            16463..17515
                     /gene="dusB"
                     /locus_tag="PKL42_02080"
     CDS             16463..17515
                     /gene="dusB"
                     /locus_tag="PKL42_02080"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HNU65887.1"
                     /translation="MQIGKHILKNNLVVAPMAGVTDRPFRQLCKKMGAGLAVSEMVTS
                     NSLLYGSEKTKRRANHEGEVDPISVQIAGADPKMMAEAARHNVDNGAQIIDINMGCPA
                     KKICNVMAGSALLKDEPLVAKILKAVVNAVDVPVTLKIRTGWDKNNRNAVQIAKIAED
                     LGVQALAMHGRTRACLYTGDAEYDTIAAVKQAIKIPLIANGDITTPEKAKFVLDYTGA
                     DAVMIGRAAQGRPWIFREIDHFIKTGERLPPPEVTEIRSVMLAHLHDLYAFYGELTGM
                     RMARKHISWYTKGLKDSANFRHSMNTLQTIDLQLSAIDAFFNHLQTRDHRLQYDEQDE
                     SNQSEFEINNLSLKVA"
     gene            17515..17748
                     /locus_tag="PKL42_02085"
     CDS             17515..17748
                     /locus_tag="PKL42_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNU65888.1"
                     /translation="MNKSELAKSVKSSLDQYFKDLDGEPPHALYDMVLACIEKPLLEY
                     TMQYVNGNQSKAAEVLGLNRNTLRKKLQIYKID"
     gene            17799..19406
                     /gene="purH"
                     /locus_tag="PKL42_02090"
     CDS             17799..19406
                     /gene="purH"
                     /locus_tag="PKL42_02090"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNU65889.1"
                     /translation="MATIKRALISVSDKTGIIDFAKNLSALGVEILSTGGTAKLFRNH
                     SIPVIEVSDYTGFPEMLDGRVKTLHPKVHGGILGRRDLPEHVSAMQAQGIPNIDMVIV
                     NLYPFEATVAKPDCTLEDAIENIDIGGPAMVRSAAKNWKDVAVITDASQYADIVRELQ
                     STGGALAEATTFALSVAAFNRVSQYDAAISNYLSAIDYNASQSSSKPVLAAFPAQSNT
                     NFIKVQDLRYGENPHQSAAFYRDLYPAPGSLVTAQQLQGKELSYNNIADADAAWEAVK
                     SFDSTACVIVKHANPCGVALGKDALEAYSKAFQTDPTSAFGGIIAFNTTLDKATAEAV
                     SKQFVEVLIAPDYTADALEIFKAKANVRVLKIALPEGGNSNWEKGRNSHDTKRVGSGL
                     LIQTADNHEMHISDLKVVSKKQPTPAQLQDMLFAWRVAKYVKSNAIVFCGNSMTLGVG
                     AGQMSRVDSTKIAAIKAQNAGLTLKNSVVASDAFFPFRDGIDVLAEAGAACVIHPGGS
                     MRDEEVIVAADEHGLVMVVTGIRHFRH"
     gene            19448..20716
                     /gene="purD"
                     /locus_tag="PKL42_02095"
     CDS             19448..20716
                     /gene="purD"
                     /locus_tag="PKL42_02095"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNU65890.1"
                     /translation="MKILVIGSGGREHALAWKVTHNKQVSRVYVAPGNAGTALDPDML
                     NVPITAVADLVKFAQDEQIHLTIVGPEAPLSQGVVDAFRAAGLKIFGPTKAAAQLESS
                     KDFAKAFMVRHNIPTAKYETFTDAKQAHDYVNDNGAPIVIKADGLAAGKGVVVAMTLD
                     EAHAAIDAMLEDNKLGAAGARVVIEEFLTGEEASFMVMVDGKNILPLATSQDHKRLQD
                     ADLGPNTGGMGAYSPAPVVTPTIHAKVMREIIKPTVEGMANDGIPYTGFLYAGLMISP
                     NGDIKTLEFNCRMGDPETQPIMMRLKSDLVGLMEHAVNGTLNLAEADWDRRFALGVVM
                     ASANYPDTPRLGDEITGLPKHLTDAHVFHAGTVLEDDKVVTSGGRVLCVTALGETVKF
                     AQQQAYKIASEIHFNGAQYRKDIGYRAIKG"
     gene            20723..21643
                     /gene="hemF"
                     /locus_tag="PKL42_02100"
     CDS             20723..21643
                     /gene="hemF"
                     /locus_tag="PKL42_02100"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HNU65891.1"
                     /translation="MNTTDVFNYLQNLQAKIVEAIELVDGKNFLQDSWQRPEGGGGNS
                     CLLEGGNVFERAGIGFSHVLGTKLPPAASVAHPEAAGRPWEAMGVSLVFHPRNPYVPT
                     VHMNVRFFVAKAQNDAEQDIWWFGGGMDLTPYYGFAEDAEHFHRTNKTALDAFGTSYY
                     PKFKKECDAYFYLKHRQEPRGIGGIFYDDFNELGFERTFDFHRAVGDAFLQAYLPIVQ
                     RRKDTPYGERERDFQAYRRGRYVEFNLIYDRGTIFGLQSNGRTESILLSMPPIVKWRY
                     DWKPEAGSAEAKLYTDFLIEKDWLSLNKAS"
     gene            21640..>22783
                     /locus_tag="PKL42_02105"
     CDS             21640..>22783
                     /locus_tag="PKL42_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HNU65892.1"
                     /translation="MKITPQTRALAQDLLNFIDASPSPWHVVETAKQHLLAQGFSSLS
                     EADPWQLKAGAKYFVTRNDASIIAFTLGSEDLVESGFRIVGAHTDSPGLRLKPQAAFG
                     SDGLVRVGVEVYGGPILATFTDRDLSIAGRVMVRTASGHETKLIKFEDTLMRLPNLAI
                     HMNREVNDKGLKLDKQKELPLIFGESASGFEADQQFLAHIAKALKVEVHHILSFELNV
                     FDTQKGAFWGAEQAYIANSQLDNLASCHAALTAMLASHNSATTNICALFDHEEVGSES
                     ASGASGSFLNDTISRIAIATGLNAEDKQRAFAQSFFVSADMAHAYHPNHPGAYEPCHH
                     VMVNHGPVIKTNANQRYSTNADTAARFMTLCEKANVPFQQYAHRTDL"
BASE COUNT         6527 a         5306 c         5074 g         5876 t
ORIGIN      
        1 cttgcagagc atgcaaaaag gttgtgggtg aaataagcca aaaatgtgct tttttgcaat
       61 cattatcagc ctaaattccc agcttctacg ctcaaaaaaa atccctgcat ttgcagggat
      121 ttttaatgag ttgcattgaa caacatcgac aagaaaaatt tatttcttta cttttttctt
      181 ctttgtagtt gttgtctttt taacaggctc ttttaccacc ggctcttctg cctgcaccgc
      241 taagttgccg cccgcaccca aaccaccttc aactgtttgt gttttgtagt ttttaacggc
      301 tttcacaagg ttattgtatg agtcagtaaa cgcagcaacc accactttac cttcaggcgt
      361 tttagtgtaa ccgccacccg ctgcgcccat acggctgcca aacgcgccgc caaacaagct
      421 aaagtcagtg tttctagcgc taccttctgc cgcagcgact tgaacgctac tacggttatc
      481 cactaaggtg agcatggtgc tggcatcggc aaacttaagg ccgcctgcca gtaaacctaa
      541 ccagccgcca ccccaaccgc ctaaaccagc gcctacaccg cccgtgtcgt tgctgctaaa
      601 tgtaatggtt gggttcatgg cgtaatctgc tgctaccatt tggccttttt tgaagttgct
      661 tgtttcgcgc atttcgccgc tgtcgcgcag gttacgttcg ccattgccta gtgccatggc
      721 cgcgccacgg tcaaccacca caaagcagtt agattgctgt actaacaatt taagcactgg
      781 tgtggttggg cctaagtgat attggccggt taattgtctg taccaatctg cattggtgtc
      841 ttcaaccacg gctaaagtgc ccagtggttt atcgcatttt tctagtgttg cgttggcatt
      901 cactgcattg ccgccacccg ctgaacctgt ggcttctgtc ttggctttag agctgcccat
      961 atccatggtt gcacagcccg tggccgccaa catcatagtt gctgaactta ccgccatcag
     1021 catcgtttta agttgaaatt gcatttttac ccctttttaa tgttcgtttc gataacaaat
     1081 ggactacgat tatttttctg atgaccgcta ggcgtggaaa cattttcaga attatgaatg
     1141 ggataatatc ataaatttat aatcgcataa cacactaaca ggctattgaa aaccctactc
     1201 tagtatggtt tggccaaaaa aaagccccaa ctcaagggga taagagttgg ggcaaaatgc
     1261 cttattgtca aggcttccgc ttagggagga aagagcggaa aggataacca aaattcattt
     1321 tagctatcat ggtaactatg agtagtcgca acccttgtta gttcgctttt ttttgtaaca
     1381 ctttgtatcg ctttgtaaac ggttaacacc caaaatttta gtgaatccca tgcgataata
     1441 tcgttatgga aattgaacat tctccccaat ccccaagtac gcttttagct gggcttaaca
     1501 ataagcaact tgaggccgtc acactgccgc accaatctgc acttatactc gcaggtgcgg
     1561 gtagcggtaa aacgcgcgta ctcacggcgc gtatcgcttg gctgattcaa accggccaag
     1621 tctcaccaac aggtttactg gcggtgactt ttactaataa agcggccaaa gaaatgctca
     1681 cacgcattac agcatcgctt cccattaaca cgcgcggtat gtgggtgggc acttttcacg
     1741 ggttgtgtaa tcggttatta cgcacgcatc accgcgaagc tggcttgccg cagagctttc
     1801 agattttaga tattgctgat cagctttcag tcatcaaacg tttgatgaaa ctgatgaacg
     1861 tggatgatga aaaatatccg cccaagcaag tgcagaacta tatcaacggc tgcaaagatg
     1921 aaggcctgcg cgcccatgca gtagaagctt acgacgcaca ttcgcaaaaa atgcgcgaga
     1981 tttttgacgc ttacgacaag caatgccagc gtgatggcgt ggccgatttt gccgagcttt
     2041 tattgcgctg ttacgagcta ctggagcgcg atgccaacat tcgccagcac taccaaagcc
     2101 gctttaaata cattctagtg gatgagtttc aagataccaa tcgcctgcaa tacctgtggc
     2161 taaaactcct cgcaggcctt ggtaactgca tgttcgccgt gggcgatgat gaccagtcga
     2221 tttatggctt tcgcggtgcg cgcgtgggca atatgcgtga ttttgaaaaa gatttcaacg
     2281 tacagaatat cgttaagctt gaagaaaact atcgatcaca tagcaatatt ctggatgccg
     2341 cgaatgccat tatttcgcac aatcacaatc gtttaggtaa aaacctatgg acatctgcag
     2401 gcgcaggcga gccggtacgc gtgtacgatg cctataacga caccgatgaa gcgcaattta
     2461 ttgtcgatga aattaaaatg ctgcattgcg aaggcacctc ccttggcgaa attgcgttgc
     2521 tctaccgctc aaatgcgcaa tcacgcatct tggagcatgc cttattttct gccaatttac
     2581 cttatcgggt atatggcggt ttgcgctttt ttgaacgtgc cgaaattaag catgcgctgg
     2641 cctacatgcg cctcattgcc aacgccaatg acgacacggc attattgcgc gtcattaact
     2701 ttccagcgcg cggcatcggt gcgcgcagtt tagagcaatt gcaagaagcc gcacgcgcgc
     2761 aagattgcag catttggcag gctgccatta acaaggttgg caatggcaag ttggggggca
     2821 aaggcattga aggctttgta gcgctgattc gccaaatgat tgatggcgca tacggcatta
     2881 gcttgtctga attgacggaa ctcgctatca atcaatctgg cctgatggcg cattatgaaa
     2941 acgacaaaga aggtgaagac cgcatcgaaa acttgaaaga gttagtcacg gccgcagtgt
     3001 catttaccaa tcaggatttt ggtaaccaca ataatgtaga tggtgacacc gagcaagatt
     3061 tactcacaca gtttttaagc cacgcaagct tagaagctgg cgaacaccaa gccgatgtcg
     3121 ggcatgaagc cctgcaactc atgacggtac acgcctcaaa aggcctagaa ttcaaagtgg
     3181 tatttatcag cgggcttgaa gaaggactat gcccgcacga gcaaagcttg tatgaaaatg
     3241 ctggcttaga agaagagcgg cgcttgatgt acgtggccgt cacgcgtgcg cgccaacggc
     3301 tgtatttgag ccacgcccaa agccgcatgt tgcatggcaa agtgcgctac ggtattccgt
     3361 cgcgcttttt agatgaaatc cctgaaaaac ttctgaagcg attgaatagc aaacctgtta
     3421 gcaaaccaag ctacagccgc gactacaacg aattaccgaa aatcatgaac acgcagcaaa
     3481 gtagtgacca aaaaagcgct atgccctgga aaattggcca gcaagtagcg catgcaaaat
     3541 ttggtaacgg cgtggtggtg agttatgaag gcaacgccag tgatatgcgt gtgcaggtaa
     3601 attttggccg tgaaggctta aaatggctgg cgatggaatt tgcaaaactc acgccgattt
     3661 agcgaagttg atcaattgaa acacttagaa aatggggtga tttttacacc aacctttttc
     3721 gatgctctgg aagccttgta aataaaggct cgcagagcat aaaaattttc tcgagagaat
     3781 ttagcgttaa aaaagcttaa ttttgctcaa tgccaaatat atctaaatag ctggtataag
     3841 tgctggtaat catgagcggc accaccacga gcaaccctaa gaacagcgga acaatggcaa
     3901 gaatagtgag tacgccaaaa atcaaaatgt aggccaacac gggtaacaca ttgcgccaaa
     3961 ctgcaataaa gctgattttc atggcatcaa ccgctgttag ctgatgcaaa cgtaccaaga
     4021 gcggtgcaaa ccaataagcc atcagcactg gcagcatcaa cagcaacgca ataaaggtac
     4081 ttggctttaa caggctgccg gcttgttctg gcgtaagttc ttgcccgctg gcaatgaggg
     4141 cgacgatttc ttggtctagg ctcgtcacca caatcacgga taccattagt agcgccatca
     4201 tgtacaaacc gcctacggca acaagttgcc ccgtattttg tttgaagcct gcaaacagat
     4261 gattgatttc taattcttga tgccgccgaa tagcgaggca acccatcatc atgcctgccg
     4321 caaaaatagg ggcaagcagt gcgccaaaca ccgtacccaa tagcggcacc agcgagacgg
     4381 gaactgcaat ggccaaataa atcacaaata gcacaatcca catcaacggg tatgctttaa
     4441 aaagctgaaa accgctcaca atccaagccc atgcgtggct ggcagggact tgtcgaatgt
     4501 ttaaaggggt tgattctggt ggattgttac gttggggttc tgtcatgtta cacgtcctta
     4561 gccttggcgg cagcggcctg tagcgcaatg cgctgctgta atatctgctt aaaatgattg
     4621 gggtctttgg cgtgagtgag ctcgccttct gccggaaaat gaaaatcata caagcgagac
     4681 aaccaaaaac gtaatgccgc gcggcgtaac aatgcatgcc aacttaggca ttcggcctcg
     4741 ttaaaagggc gcactgtttg ataagcggct aaaaatgttg ccagcttttc ttcgtcaaaa
     4801 taaccatccg gcaaaatgca ccaatcattg acagcaattg ccacatcgta ggccagacca
     4861 tcatggcagg cataataaaa atcaataaaa ccgcctagct tgtcaccatc aaacagcacg
     4921 ttatcgcgaa acaagtcgcc gtgtaccacg ccgtgcggca gattgttcat cgcaaaattt
     4981 tgctgataat caagttcggt ggtcaataat tgttgttctt cttcacttaa ttttgcaaat
     5041 acattttgcg cggttaccct acgccaatcg gcatcacgtt gattatgaat tgtattgcct
     5101 aactgaaagt gctgtggaaa ttgcgcttca aatgtttgcc ctgccaaatg catttgcgcc
     5161 agcacgcggc cgacttctgc acactgcgct aaattgggct gctgaacatc ttgccccacc
     5221 aaacaactca ccaaagcagc gggtttgcca ttgagcacac gcaaacaaac gccctgaaga
     5281 tcagcaatcg gtttggggca tggaatgcca tgtgcagcca aatgactcat caaatcaata
     5341 taatagggca attcttcaat attattttgt tcaaataatg tcagcacgta tttggttgta
     5401 accccgtcat ttgtctgagt tgttacaaaa taattggtat tggtaatgcc cgatgaaatg
     5461 ccttttagct caacaagcgc gccaattgaa taattttgta gccaagtgct aagttcttgc
     5521 tgagtgatgg tggtaaaaac agacataaat aaaggctata aataaagact aggagtaaag
     5581 gctaggaata aaggctcgga taaaatcggt gaaatgaatt tttttgaaca ttacaaacaa
     5641 ataaaatttg aaatacatgt gccgagaagt ggaacgcctc tcggcattgt tggtttaaaa
     5701 cctaaatatg gtccatttag gaatgctgac tggctgaact ggcccgtaat taatccattc
     5761 gccatcttgg tcttctttat gcaagtaata aggcacacca tgtgcggggg tgattttcat
     5821 catgtaaagc tggccattga cacgatactc ttcaatcgtc tcgccctttt tcttcacaat
     5881 ggtaatttcc ggctcgtcca cattatcatc tttgccaatc accggagggg gaatatcctc
     5941 tagcggtacc gcatctgctg gcgtcggtgt tggttgtggt gctttcgacc tagcctcttc
     6001 agccattaca gaaaaaggca ttgtcaccaa agccaataaa cccgctatca accaaaaatt
     6061 tttacgcatt ttttctgcct tttctttgtt caggaactca cgctttattc taacaaattt
     6121 aacgtctgta aaaccgctgg gtttacaaat ataactgtac acgttcctca gcggccgata
     6181 atttaaagcc gcgagtctcg taatgtttaa aaatggcctc tacaatttta gcgggctcgt
     6241 caataatctg tattaagtcc aaatcttcag gtgaaatcat tttttcattc accagcgtcg
     6301 ttttaaccca gtcaatcaaa ccgcgccaaa atggctcaca gaccaaaatg atgggcattt
     6361 tgagcgtttt gcccgtttgt atgagcgtaa tcgcttccat cacctcatct aaggtaccaa
     6421 acccgccagg catcactaca taggcgctgg cgtatttcac aaacatcacc ttgcgcataa
     6481 agaagtgctt aaagttttga ctcacatcct gatatggatt ggcctgctgt tcatgtggca
     6541 actcaatatt gaggccaatg ctcggcgcct tgcccggaaa cgcgcccttg ttggccgcct
     6601 ccataatgcc agggccgccg cctgaaatta ccgcaaagcc agcgtcactc agtaaccttg
     6661 cgatttcttc tgtgagctta taataagggt ggtcttcagg ggtgcgggca ctaccaaaaa
     6721 ttgaaacggc cggtgtaatt tctttcaggc gttcggtcgc atcgacaaat tctgccataa
     6781 tctcgaatgc atgccatgat tcgtgccccg tgtgatgcaa gccttgcaca tctggatcag
     6841 taatttttgg aacttttttt gcaaaagaca taatttaggt acccataaaa catgaaaacg
     6901 ttgttattag ttgatggttc atcttacctg tatcgcgcgt ttcacgccat gcccgactta
     6961 cgaaatagcc gtaacgaacc cacaggcgca attcaaggtg tgctcaatat gctgcgccgt
     7021 ttgcataaag attacccttc tgattatagc gcgtgtgttt tcgatgccaa aggcaaaacc
     7081 tttcgtgatg acatttaccc tgaatataaa gccaaccgcg ccagcatgcc agacgattta
     7141 cgtgcgcaaa tagaaccatt gcatgaggcc attaaagcca tgggctggcc gctcattatg
     7201 gaagatggcg ttgaagcgga cgatgtgatt ggcgcactgg ctaaacaggc cgagcgcgaa
     7261 ggtgtgcgca cgattatttc cactggcgat aaagatatta cgcaattagt aaatgagcac
     7321 atcaccgtgg taaacaccat gcgcgatgct tttcgtaaag tggatgacgt actggatatt
     7381 gcgggcgttg aaagaaaatt tggcttacca ccaagtttga tcattgatta tctgatactt
     7441 gttggtgaca ctgctgacaa cgtaccgggg gttgaaaaag ttgggcctaa aaccgctgtg
     7501 aaatggctaa ctcaatatgg ttcgctggaa aatattatcg ccaatgcaga ccacattact
     7561 ggcgttgtgg gggaaaatct acgcaaagca ctgccttggt tacccaccgc gcgtgagctc
     7621 attaccattc gttgcgatgt tggcattcaa gaaaatttga acgatttagc gccccaactg
     7681 caagacaaag aaaagttact agcactgttt gaccgctttg aatttaagtc ttggaagcgt
     7741 gagttagacg ccgagggcaa aacacctgcc ctgcaagcca atgtttacaa ggcttccagc
     7801 gatggtcaaa aaggtttatc tcaaatacca gcagaaattg cttctgaagt tagcgcttac
     7861 tctcccaacc tttcacgcca atacgaaact atttttagcc cagcacaatt agaaatttgg
     7921 ttagaaaaat tatcaaaggc tgtcctgatt tgctttgaca ccgagaccac ctcgctcgac
     7981 ccgttgactg ccaaaattgt gggtatgtcc ttttcggttg aagccggcag tgccgcctat
     8041 ttgccattaa agcatgatta ttttgatgcg ccagaacaac taaattttgc tgaagcgtta
     8101 gccaaagtaa agcctatttt ggaaaatgcc aacattaaaa aagtgggcca aaaccttaaa
     8161 tatgaccagc atgtattagc caatcacggc attatgctca atggcattgc gcatgacacc
     8221 atgctgcagt cttacgtgtt tgagtcgcac aaaacccacg gtatggacgc cttgagtgag
     8281 cggcatttag gcattacgcc catcagtttt gaaagtgtgg ctggcaaagg tgccaagcaa
     8341 gtgggcttta accaagtgac ggtagaggtg gccgcagagt acgcagcgga agacgccgat
     8401 attaccttgc aattacatga agcgatgcac ccaatcatcg cggccgatgc caaactgctt
     8461 tatatttacc agcagattga aatgcctgtc tctgaaattt tattcacaat cgagcgcaat
     8521 ggcgtgctga tagatgccaa tatgctcaac agccaaagta atgaaattgg cttaaagttg
     8581 attgcacttg aaaatcaggc ctatgaattg gcgggccagc cgtttaattt ggcctcgcct
     8641 aagcaattac aagaaatttt gtttgataaa ttaggcatca aacccaccaa aaaaacaccc
     8701 agtggtgcac caagtaccga tgaagatgta ctgcaagaac ttgccctaga ttacccgctc
     8761 cccaaattgt tgctggaata ccgtggcttg gcgaaactga agtctactta caccgacaaa
     8821 ctgccaaaaa tgattaaccc gcagactgga cgtgtacata ccagctacaa tcaagccgtg
     8881 gcgattacgg gcaggttagc gagttcagac cccaatttgc aaaatatccc tgtacgcact
     8941 gctgaaggcc gccgtattcg cgaggctttt attgcgccac cgggcagcca tattgtgtct
     9001 gccgactatt cgcaaattga gttacgcatc atggcacatc tttcaaaaga tgcgggcatg
     9061 ctacaagctt ttgccaataa tgaagatatt caccgccaca ccgctgccga gatttttggc
     9121 gtggaacgag acgccgtcga taacgagcag cgccgttatg cgaaagtgat caacttcggc
     9181 ctgatatacg ggatgagcgc atttggtctt gcgcaaaatc tcaatatcga gcgcagtgcc
     9241 gccgccaact atattgaacg ctactttgcc cgttaccccg gtgtgcgtga gtacatggat
     9301 aaaacccgcg atattgccaa acaaaaaggc tacgttgaaa cctactttgg ccgcagatta
     9361 tgggtgccag aaatcagcag ccctaacggc atgcgccgcg ctggcgctga gcgcgctgcc
     9421 attaatgcgc caatgcaagg cactgcggca gatttaatca aactggccat gattgcggtg
     9481 gatatttggc tacgcgatga gaagctaaca accaagctaa ttatgcaggt acacgatgaa
     9541 ttggtattag aagtgccaga gaatgaactt gaattggtaa aagtcaaatt accggaattg
     9601 atgcaaaacg tggctaagtt agatgtgccg ttgttagcag aagtgggcgt tggtagcaat
     9661 tgggaaagcg cgcattagcg gttacatact cgcatcataa aaatcaccca caggcgcatc
     9721 gcgattcaat acgatgcagc ctttaatcac acgataccaa aaccataaag aagcaataac
     9781 aaacattgca aaaccaatca gcatgaaagg gttgaaaaaa gatccaatcg cctcattcaa
     9841 agacctagct tgcagacctt tcgcactcgc tgtcagaaat gaggcgaaca ccaaaaatgc
     9901 gcccaagcaa ccccacagta gcgaaaacca aaaagtgcta atttgccacg cataatgcga
     9961 ctgcacccaa ttttgtttgc ccatatcttt acgatgcata tgcgctaaaa caatagcggc
    10021 caaacctgaa acgcccccaa aaatagaacc aaaatataaa atatagatta aatttgcgat
    10081 tgacttatca gacttgatct tggcgacttc tttaacatca atcggttggt tggttttcac
    10141 aaactcaaca cgctcaattc tattcagtag cgcttcatct gcctgcctct cgcgctctgc
    10201 ggcttcggcc tttgcatata ctactccgca gctagggcaa gcccattctg gagtattgtc
    10261 atctgttgct tgacgttgat aattgcactt gatgcaggtt ttaccttcat acaccaacgc
    10321 gaattcactc ggggttccgg agtttctgtt ctcttgcacc atgattactc caccttaggt
    10381 attcgacgca ttaaacaaga ttgccacacg ctaaactttt cgcaagccat caaacataat
    10441 aaccagattt cgccctgcct ttttagcctg atacattgcc tcatcagcat tttttagcaa
    10501 aacttcgttg gaattaccat gttcaccaaa catagcaatt ccaatacttg ttgaacaatg
    10561 atgctgcagt gcgttcaaat gatacgggcg gtttagtgct atcacaattt tttctcccag
    10621 cacttctacc tgctgcatcg cctctatacg gtcctcactc aaatcctcta acatcacaac
    10681 aaattcatca ccgcctaggc gggcaactgt atcactttct cgcacgcaac ttaataagcg
    10741 ctcagccacc tgctgtagaa gtaaatcccc cataaaatgt ccatgattat cattgagatt
    10801 tttaaaataa tctaaatcca aaaacagcaa ggcgcctctc ctgcctgtac gtgcactcat
    10861 tattaatgca tgctccagcc tatcaaacag caggcgacga ttcggcaagt tggttaatga
    10921 atcataaaaa gctaattgtt taatttcatt ttcacgctcg actgcttcaa aacgtagcgt
    10981 gatactctca atcagattac gattgatatt tccgatactc gcaatcataa aaatgacata
    11041 taaaaaacca gacacggaca ttgccatagc cacgctatca ccaacaagaa ataaacgaac
    11101 aagcattgga actagcgtca gtagcataaa tataattgca ctaaccttat caactgagta
    11161 agaaatcact gccccagcgg tcatgcctgt taatacaaaa atcagaagca tttgatgttg
    11221 aagttcatta gacggagaca aaattatgct ggacgacccc catagaaatg ctgaaacaaa
    11281 caggccgata cggaattgcc ttaaacgttt agttatgagg aaattgtcat ttacttgatg
    11341 gcgtttataa ctccaggcca tgaataatcg gccgatattc actaaaacta tcaatattag
    11401 ccaagtggaa accgtcggca atgcaaccaa ataacgttgg aaataagcaa ctagggcagc
    11461 gagcaatgta ctgctgacta gtgacctacc gttagcatcc agcagttgtt gtaattgcgc
    11521 agacttgata gcaacatctt taattgaact atccatcaaa attctctcaa tggtttggag
    11581 attgatttca agtcacgcaa attctggtta tgccgaacag tcgtttgcaa tttagagacc
    11641 taaacttcct caagcccaca cactaaaata cactttgggt atgcgttaca tttcataatc
    11701 aaacattttc attaaatttc gtcctgcttt ttttgcctga tacatagcaa tgtccgcatg
    11761 cttcaataac tccacttgag agttcccatg gtctctaaat accgctatgc caacacttgg
    11821 cgtgctgtga tgctggtgct tacctagctg atagggtttg tttaatgctg ttaagatttt
    11881 atttgctcta atttcagctt gactggcggc ttctagcggc tctttactta gcccttcaag
    11941 taacactaca aactcatccc caccgattct agctactgta tcactgtcgc gcacgcaatc
    12001 ttgcaggcgt tcagccacaa gtttgagcaa taaatcaccc atgtcatggc ctaaggtgtc
    12061 gttcagcact ttaaaatgat ctagatctat gaacagtaat gcccctcttt tgcccgatcg
    12121 aacactgata gtgagtgcat gatctaaccg gtcaagcaat aatcggcggt ttggtagctt
    12181 ggttaacggg tcataaaatg ctaattgttt aatttcatcc gccgcctgtt tttgtgcggt
    12241 cacgtcatgc acaatggcaa ttccgccaac cactatacca gcactgtcaa aggctggggc
    12301 tgcggttaat tccatccatc cttccgcttc gctaatggtg gcacgatatt gcccatcaaa
    12361 agtagccact tcgcccgcta gcgctttatt aagcgttggt aaaattgcat cgtctttaat
    12421 catcaagata ttgcgattga taatttcatc ttgaggcgat tgcaaaatgt ctgcaagact
    12481 ttggttacag tagatgatat tcaagtcacc atcaaagtgt gcaataccaa ccggtgagtg
    12541 attgagcaac atgcgatatc gcgcttcact ggcagtaatt tccttttctt tttctaccgc
    12601 atccacacgc aatccaatcg tttcatgttg actttgattc acattgcgca gactcaacac
    12661 ggtaaacgcc ataaaaacaa aacctgaagc gcccatcaac atgggaaagc tttcccctgt
    12721 agctagaaag cgaattaata aaggaatcag cgcaaacaag gtgtaggcaa tcgcactaat
    12781 aaagtctatc gaatacgaat aaaaactccc cgctgaaaac cctgtcagca tgtataaaag
    12841 gagcatttgt tgctcaatat tattcgtagg aaacatgagc aaactggttg taccccaaac
    12901 cacacttgcg gcaataatcc cgatgcggaa agttttaaga cgtttttgaa tttcttgcaa
    12961 atcaaccgta ggaacttgcc ggtaataaaa accaatcaca acgcgcgcca gattaatgcc
    13021 taaaataagt gccagccaac ctttgagaat gatcggatca gtcacctcgc gctgccagta
    13081 gcaaagaatg actgccatga tagaacttgt gatgagcgtt ctattattga cagacaaaag
    13141 ctgtcgatac tgctcaaatt gaattgccgt tgttttcatt tgatttatca tttttcttaa
    13201 ttttcacgcc ctcttaaggc tgaggggcag ttcattatgc aatatcttat tttttgcttc
    13261 aaccgatttt ccgctaaatc acacgtttat ttcaagcagc ttgcatctaa agttaggcgc
    13321 tcttttgagc atcaaagctg attttttgct aaactatcgc aatgaacaaa caacaagcta
    13381 ttcaaaatct tagcaacagc aaagtcatac ttagtcaacg ctttggcgtg aatcgtttga
    13441 cgctgtttgg ctcaacagcg cgtaatacgg caactgcaat tagcgatgta gatattttaa
    13501 tgggctttga tggtgttgca gactcaagac gctattttgg tgtgcagttt tatttggaag
    13561 acttattgca ctgcccagta gatttagtca cagaaaaagc gttacgtcca gaattaagac
    13621 cattcattga gcgcgaggcg attgatgtct gattcaccta agcgtgaatg gcgattttat
    13681 attaccgata taataggttt tgccgaaaat gtcaccagct ataccgatgg ttttgactaa
    13741 gagcgttttg tgaatagcgg cattacttac gatgcaactt tgcgcagtct tgaactcatt
    13801 ggcgaggcgg caacgcacat tccaccagaa atacgtgagc aatacagcaa tattccatgg
    13861 cgaatgatga tcgccacacg caacacactc attcatggtt atttagggat tgataacgat
    13921 acgctttgga gcattattca aacggatgtg cctgaggtac ttactgaatt acaaaaaatc
    13981 taatcaatag tccccatgca aaacataggc atctacatca tcggtgacga aattctctct
    14041 ggcaaacgtc aagacgcgca tttaagcaaa gtaattagcc tactttctac ccgcggcttg
    14101 cagctgagtt gggcgaatta tttgggcgac attcccgcgc aaatcactag ctcactcaaa
    14161 gccagcatgg cgcgcggcga cattgtgttt agctttgggg gtattggtgc cacgcctgat
    14221 gattatacgc gccaatgtgc ggcagatgcg gctagcgttc caattgaacg tcatgcaggt
    14281 gcggtggcag aaattgaagc gcaatatggt gaaggcgctt acccgaagcg cgtgttgatg
    14341 gcagattttc cgcaaggctg tgaactcatc cccaacccaa tcaaccgtgt ggcgggcttt
    14401 agcattcatc agcattattt cgtgccgggt tttccgcaaa tggcacaccc gatggtggaa
    14461 tgggtgttgg aaacctacta tcaaccacta tttcatcaac aagattatgt cgaggcatcc
    14521 attttggtga tggatgcggg cgaaagtaaa ttgattgatt taatgaacca cattttgcac
    14581 caatacccaa cgctgaagct cttcagtctg cctaaactag atgatagacg caccacagaa
    14641 attggaatga aaggtgctga aaaaccagta aaagctgcaa tgcaagacat caaagaaggt
    14701 gtgtctgcgc tgggttttcc ttggtcagac ttgtgatgtt gtatcacctg aaattggaca
    14761 actaacattg gaatgtaggg caaactgtgg cgcctgctgt actgcgcctt cgattaacag
    14821 ccccatcccg ggcatgccta agggcaaacc agcaggcgtt cgttgcattc agctagatga
    14881 gctaaatcgt tgtatgattt ttggcaaacc tgaacgcccg ccattttgtg caagtttaca
    14941 gccgaatata gaaatgtgtg gtgacacgcg tgagcaagcg atgatttggt tgagcaagct
    15001 tgaagtgctc acaaaaccga attaggcctc attaaaatta cgctgctgcg tttcattcat
    15061 caaaataatg gcaaccaagc ttaccaaagc catggctgag acataatagc cgacccacac
    15121 caagccgcct ttatccacca gtaattgtga aataaatggc gctgtcgaag cgcccaaaat
    15181 tccacccaag ttatatgaca atcctgcgcc agtgtaacgt actgcaacag gaaactgctc
    15241 aggtaaaaac gcgcccatcg gtgcaaaggt agcacccatt aacagcaatg caatggataa
    15301 gaacaaggtg atttgccata gcgaaccgct agccatgagt ggagccaagc tgaaaccagc
    15361 caaaattgcc aataaacaac cgattaacaa cactggctta cgtccaaact tatcactcaa
    15421 accggctgag attggcgtaa tggctgccat aaacagcaca gcaacgcata acatttgcaa
    15481 aaagtcggca cgggcgatat tgagtgacag agtcccgtaa cttaagcaaa ataccgtagc
    15541 cgtgtaaaat aagttgtagc acaccgtcat tgccagcgca cccagtaaaa caggtcgaaa
    15601 gtgcgtgctt agtaaagatt taattggtgc tttatgcgta tgatgctgcg ctagcactgc
    15661 agcaaaagca ggtgtttcgg ctaatttgaa tcgcacatac aaccctacca aaacaagtag
    15721 cgcgcttgca aaaaacggaa ttcgccatcc ccatgactta aaagcttcat cagataaata
    15781 aagtgttaac actaaaaaac ttaaggttgc tagcaaaaat ccaactgacg ggcctagttg
    15841 tggaaacatg ccaaaccagc cgcgtttatt tgcgggcgca ttttctgtgg caagcaaagc
    15901 cgctccaccc cactcgccgc ctaagcctat cccctgacca aatctcaaca aacataacaa
    15961 aatagccgcc cacgttccta ttgattcata actgggcagg caaccaatca gcaatgtaga
    16021 tatccccatc accagtaacg aagccgctaa agtagcctta cggccaatac gatcaccaaa
    16081 gtgaccgaaa agaatggcgc caaatggccg tgcgataaag gcaattccga atgttaaaaa
    16141 agcattaagg gcctgcgctg ttgggtcgct tgctggaaaa aacacttgcc caataacgag
    16201 cgctgaagcc atcgcgtaga tataaaaatc ataaaactca attgccgtac caataaaact
    16261 ggcaaaggcg atatgtgttg ttgaggggct gctactttgc aaaattttca tattgatgga
    16321 aatgattttt aacggcgtta aattgacgcg catcggcatt atacaaactt ttagttttaa
    16381 actaattttt gtaaaaattt aagttttctg acacttcatt tgcgtataaa ataggcagct
    16441 ttctcgcaac gattacaaac ctgtgcaaat cggcaaacat attttaaaaa acaatctcgt
    16501 tgtcgctcct atggcaggcg tcacagaccg tcctttccgt caactctgta aaaaaatggg
    16561 ggctgggttg gctgtgtctg agatggtgac ttccaactcg ctactttacg gtagtgaaaa
    16621 gacgaaacgc cgcgccaatc acgaaggtga agttgaccca atttcagtac aaattgccgg
    16681 cgcagaccca aaaatgatgg cagaagcggc cagacacaat gtggataatg gcgcacagat
    16741 tatcgacatt aacatgggtt gcccggccaa aaaaatctgt aatgtcatgg cgggcagtgc
    16801 cctgctgaaa gatgagccac tggtggcgaa gattcttaaa gcggtggtca acgcggttga
    16861 tgtgccggtg acgttaaaaa ttcgtactgg ttgggacaaa aacaatcgta acgcagtgca
    16921 aattgcaaaa atagcagagg acttaggggt gcaagcccta gcgatgcatg gccgcacacg
    16981 cgcttgcctt tatacgggcg atgctgaata cgacaccatt gccgcagtta agcaagcaat
    17041 taaaataccg ctgattgcta atggcgatat tactacaccc gaaaaagcta aatttgtgtt
    17101 ggactacact ggtgctgatg ccgtgatgat aggccgcgct gcacaaggta ggccttggat
    17161 atttcgcgag attgaccatt ttattaaaac cggtgaacgc ttacctccgc cagaagtaac
    17221 tgaaattcgt tcggtgatgt tggctcatct gcatgactta tacgcgtttt atggtgaact
    17281 cacaggcatg cgtatggcgc gtaagcacat ttcttggtat accaaaggtt tgaaagattc
    17341 ggcaaatttc cgtcatagca tgaacacttt gcaaaccatt gatcttcagc tctcagcgat
    17401 tgatgcattc tttaatcatt tgcaaacgcg cgatcaccgt ttgcaatacg acgaacaaga
    17461 tgaaagcaat cagtcggaat ttgaaatcaa caacctatca cttaaggtgg cgtaatgaat
    17521 aaaagtgaac ttgccaaaag cgtaaaatct tcattagatc aatactttaa agacctagat
    17581 ggtgagccac cccatgcgct atacgatatg gtattagcct gtattgaaaa gccgttactg
    17641 gaatacacca tgcaatatgt caatggcaac caaagtaaag ctgccgaagt tttgggttta
    17701 aaccgcaaca ccctacgaaa aaaattacag atttacaaaa tagactaacc tacctttcat
    17761 ttccaaattt caattttcat ttcctttaga gttttaacat ggcaactatc aaacgagcgc
    17821 ttatcagtgt ttctgacaaa acaggcatta tcgacttcgc taaaaattta agcgctttgg
    17881 gggtggaaat tttatccact ggcggcacag caaaattatt tcgtaatcac agcatcccag
    17941 tgattgaagt cagtgactac acgggttttc cagaaatgtt agacggtcgc gtgaaaactt
    18001 tgcatccaaa agtgcacggt ggcattttag gtcgccgcga tttacctgag cacgtgagcg
    18061 cgatgcaggc acaaggcatt cccaatatcg acatggtgat tgtgaatttg tatccatttg
    18121 aggcaaccgt tgctaaacca gattgtacac ttgaagatgc tattgaaaac atcgacatcg
    18181 gtgggcctgc gatggtgcga tcagcggcca aaaactggaa ggacgttgca gttatcaccg
    18241 atgccagcca gtatgcagat atcgtgcgtg aattacagag tactggtggc gcacttgcag
    18301 aggccaccac ttttgcactg tcagtggctg catttaaccg tgtatcgcaa tatgatgcgg
    18361 cgattagtaa ttatctgtct gccatcgatt acaacgccag ccaatcatcc agcaagcctg
    18421 tgctggcggc attccccgca caaagcaaca ccaacttcat taaagtgcaa gacttacgtt
    18481 acggcgaaaa cccgcaccaa agtgctgctt tttatcgtga cttataccct gcacccggct
    18541 cattagtgac tgcgcaacag ttgcaaggta aagaactcag ctacaacaac attgctgatg
    18601 ccgatgccgc ctgggaggct gtcaaatcat ttgacagcac agcctgtgtg attgtaaagc
    18661 acgccaaccc atgtggcgtg gcgcttggta aagatgcgct tgaagcttac agcaaagcat
    18721 ttcaaacaga ccccacatcc gcgtttggtg gcatcattgc atttaatacc acgttagaca
    18781 aagctacggc tgaagccgtg agcaagcaat ttgtagaagt actcattgcg ccagattaca
    18841 cagctgatgc cttagaaata tttaaagcaa aagccaatgt acgcgtactt aaaattgcct
    18901 tacctgaagg cggtaattca aattgggaaa aaggccgtaa ctcgcatgac accaaacgtg
    18961 tgggctctgg gttattgatt caaactgctg ataaccatga aatgcatatt agcgatctca
    19021 aagtggtcag caaaaaacaa ccaacaccag cacaactgca agacatgctg ttcgcatggc
    19081 gtgtggcaaa atatgtcaaa tcaaacgcga ttgtgttctg tggtaacagc atgactttag
    19141 gcgtgggtgc aggccaaatg agccgtgttg acagcacaaa aatcgcggca attaaggcac
    19201 agaatgctgg cctaacactt aaaaattccg ttgtggcctc tgatgctttc tttcctttcc
    19261 gtgatgggat tgatgtgctc gctgaagcag gcgcggcttg tgtgattcac ccaggtggca
    19321 gcatgcgtga tgaagaagtg attgtagctg cggatgaaca tggcttagtg atggtggtta
    19381 cgggtattcg tcactttaga cactaagcaa cctgcatact caacaatata ttcaaaggcc
    19441 agtgagcatg aaaattttag tgattggtag tggcggacgt gaacatgctt tggcttggaa
    19501 agtgacgcac aataaacaag tcagccgtgt atatgttgcg cccggaaacg ctggcacagc
    19561 attagaccca gacatgctga atgtgccgat tacggcggtt gctgacttag ttaagtttgc
    19621 gcaggatgaa caaattcatc tcaccattgt cggccctgaa gccccactat cgcaaggcgt
    19681 ggtcgatgcc tttcgtgcgg ccgggctcaa gatttttggc ccaacaaaag ctgcagcgca
    19741 actggaatct tcaaaagatt ttgccaaggc ctttatggtg cgccacaata ttccaacggc
    19801 caaatatgaa acctttaccg acgccaaaca agcgcatgat tatgtgaatg acaatggcgc
    19861 tcccattgtc attaaagcgg atgggttagc tgcaggtaaa ggtgttgtgg tggcgatgac
    19921 gttagatgaa gcccatgctg ccattgacgc catgcttgaa gataacaaac taggtgctgc
    19981 cggtgcgcgc gtggtgattg aagagttttt aacgggcgaa gaagcgagct tcatggtgat
    20041 ggtagacggt aaaaatattt tgccgttggc caccagccag gaccacaaac gtttacagga
    20101 tgccgacctc ggccccaaca ctggtggcat gggtgcatac tcacccgcac ctgtagtgac
    20161 acctactatt catgcaaaag tgatgcgtga aatcatcaag cctactgtag aaggtatggc
    20221 gaatgatggc attccctaca ccgggttttt atacgccggc ttaatgatta gccctaatgg
    20281 cgatattaaa actttagaat ttaactgccg tatgggtgac ccagaaaccc aacccattat
    20341 gatgcgccta aaaagtgatt tagttggttt aatggaacat gcagtgaatg gtacattaaa
    20401 tcttgcagaa gctgattggg atagacgctt tgcactaggt gtggtgatgg cgtctgccaa
    20461 ctatcctgat accccaagat taggtgatga aattactggc ctaccaaaac atctcactga
    20521 tgcgcatgtg tttcatgcag gcacagtgct tgaagatgac aaagtcgtca caagcggcgg
    20581 ccgtgtgtta tgcgttaccg ccttaggcga aaccgtcaaa tttgcgcagc agcaagccta
    20641 taaaatagct tctgaaattc actttaacgg tgcccaatac cgcaaagata ttggctaccg
    20701 cgcaattaaa ggctaaattt taatgaatac aacggatgtc tttaattacc ttcaaaactt
    20761 acaggcaaaa attgtagaag ccattgaatt ggtagatggc aaaaactttt tgcaagatag
    20821 ctggcagcgc ccagaaggcg gtggcggcaa ttcatgcctg cttgaaggcg gcaatgtatt
    20881 tgaacgtgct ggcattggtt tttcacatgt attaggcaca aaattaccac ccgcagccag
    20941 tgtggcgcac cctgaggccg cgggtcgccc ttgggaggcg atgggcgtat ctttagtgtt
    21001 tcacccgcgt aatccttatg tacccaccgt acatatgaat gtacgatttt ttgtggcaaa
    21061 agcacaaaat gacgctgaac aagacatttg gtggtttggc ggtggcatgg atttaacgcc
    21121 ttattacggc tttgcagaag atgccgagca ttttcaccgc accaataaaa ctgcgttaga
    21181 tgcgtttggc acaagctact acccaaaatt caaaaaagaa tgtgatgcgt atttttatct
    21241 aaaacatcgt caagagccgc gcggtatcgg tggcattttt tatgatgatt ttaacgaact
    21301 tggctttgag cgcacgtttg attttcatcg cgctgtagga gacgccttct tgcaagctta
    21361 cctgccaatt gtgcaacgcc gaaaagatac accgtatggc gaacgcgaac gcgactttca
    21421 agcctaccgc cgtggccgct atgttgaatt taatttaatt tacgaccgcg gtacaatttt
    21481 tggcttgcaa tccaatggtc gcactgagtc tattttgctt tccatgccgc ccattgtgaa
    21541 atggcgctat gactggaaac ctgaggccgg cagcgcagaa gctaaacttt atacggactt
    21601 tttgattgaa aaagactggt tatctttaaa taaagcctca tgaaaatcac ccctcagacc
    21661 cgtgcgctag cgcaagattt actcaatttc attgatgcta gcccaagccc gtggcatgtg
    21721 gttgaaacag ccaaacagca tcttttagca caaggctttt catctttaag tgaagcagac
    21781 ccctggcaac tgaaggctgg tgctaaatat ttcgtcacgc ggaacgatgc atcaatcatt
    21841 gcatttactt taggtagtga agatttagtt gaatcaggct ttcgtattgt tggcgcgcat
    21901 actgattctc ctggcttgag gctcaagcca caagcggctt ttggtagtga tggcttagta
    21961 agagttggtg ttgaagtcta tggtgggcca attttagcca cctttactga ccgcgattta
    22021 agcattgccg gacgtgtgat ggtgcgtacg gccagcggcc atgaaaccaa actcatcaaa
    22081 tttgaagata cgctgatgcg cttgccaaac cttgccattc acatgaaccg tgaggtaaat
    22141 gacaaagggt tgaagttaga taaacaaaaa gagttgccac ttatttttgg tgagagtgct
    22201 tcaggatttg aagcagacca gcagttttta gcgcatattg caaaagcact taaagtggaa
    22261 gtgcatcata ttttaagctt tgaactcaat gtgtttgata cgcaaaaggg ggcgttctgg
    22321 ggtgcagagc aagcgtatat tgccaatagc cagctggata atttggcttc atgccatgca
    22381 gcgctgaccg caatgcttgc cagccacaat tcagccacca ccaatatctg cgcactgttt
    22441 gaccatgaag aagtcggtag tgaaagtgcc agcggcgcaa gtggcagctt tttgaatgac
    22501 accatcagcc gtattgcaat cgccacaggc ttaaatgcag aagacaaaca gcgcgcattt
    22561 gcgcaaagtt tttttgtcag cgccgatatg gcgcatgcct accatcctaa ccacccgggc
    22621 gcgtacgagc cttgccacca tgtaatggtg aaccacggcc ctgtgattaa aaccaacgcg
    22681 aatcagcgtt attctaccaa tgccgacacc gccgcaagat tcatgacatt gtgcgaaaaa
    22741 gctaacgtgc catttcaaca atatgcacat cgcactgatt tgg
//
LOCUS       DAOQSI010000015        21650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_1634_length_21650_cov_6.505997, whole genome shotgun sequence.
ACCESSION   DAOQSI010000015 DAOQSI010000000
VERSION     DAOQSI010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 21650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21650
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1634_length_21650_cov_6.505997"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(326..1753)
                     /locus_tag="PKL42_02110"
     CDS             complement(326..1753)
                     /locus_tag="PKL42_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNU65893.1"
                     /translation="MKKILSTLALATMLGFGLTGNVLAEEAPVVDAAPTEMVATPAEA
                     APVETVAPEALPAEAAVPEVAPTVDKGDTTWMMISTILVVLMVPGLALFYGGLVRSKN
                     MLSVLMQVFVVFSLVSVLWAVYGYSLAFGSGSGPIIGDFGKLFLAGVTSDSLSDTFTA
                     GVKIPEFVFIAFQCIFAGLTATLIVGSFAERIKFSAVLLFSAIWFTLSYIPIAHMVWG
                     AGGYLLGQGALDFAGGTVVHINAAVAGLVGAYMVGKRIGYGKEALTPHNLTLTMLGAA
                     LLWVGWFGFNAGSNLEATGGAGLALVNTLFATAAAVLAWSIGEAFTKGKASMLGAASG
                     AVAGLVAITPACGSVGPMGGIIIGFAGGFLCLWGVTGLKKILGADDSLDVFGVHGVGG
                     IVGALLTGVFTAPSLGGTGKADFSIASQVLIQGEGVIITIIWSGIAAFIAYKIADLIV
                     GLRVSEEAEREGLDTTSHGERAYNI"
     gene            complement(1764..2102)
                     /gene="glnK"
                     /locus_tag="PKL42_02115"
     CDS             complement(1764..2102)
                     /gene="glnK"
                     /locus_tag="PKL42_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNU65894.1"
                     /translation="MKFISAIIKPFKLDEVREALSEIGVQGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKVEIAIDDKILDQVVEAIEKSAATGKIGDGKIFVFNLEQVTRI
                     RTGETGSEAL"
     gene            complement(2142..2930)
                     /locus_tag="PKL42_02120"
     CDS             complement(2142..2930)
                     /locus_tag="PKL42_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HNU65895.1"
                     /translation="MRNSLLSLAVLSVFTLPAISHADEAAPEATVVPAPASPHAFAYN
                     VGLYSQYIFRGLTQTREDPAIQGGVDYTHASGFYAGAWASNISWLTDGDYYKSSSLEL
                     DLYGGYANTIGETGIGYNVGLLQYIYPGRKFSGTKKAETTEIYGSLSYGWLSGKVSAV
                     VSDGAFGFDNADGSTYTELNLAVPFGSGFSGVAHVGYQDFTGKGNSQFDYADWKLGVT
                     KSWESGINIGAYFTDTDGKKSDFTDASGQNIAKEQFTVFVQKTF"
     gene            3108..3347
                     /locus_tag="PKL42_02125"
     CDS             3108..3347
                     /locus_tag="PKL42_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HNU65896.1"
                     /translation="MLSSDKLNELSLKIKEIISSSPLGDAEKNIHALVKGALTKMELV
                     TREEFDVQAEVLRSTKEKLSQLEAKLAELEAQQKK"
     gene            3349..4842
                     /locus_tag="PKL42_02130"
     CDS             3349..4842
                     /locus_tag="PKL42_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNU65897.1"
                     /translation="MSLAVLYSRALSGMDAPEVVVEVHLANGLPSFTIVGLPEAEVKE
                     SKDRVRAALQTAQFEFPARRITVNLAPADLPKESGRYDLPIALGILAASGQIPTEALS
                     RYEFAGELALTGELRPIRGALAMAFKSYKSGRAFILPHESAAEAALVADAIVYPANTL
                     LEVCAHFTGRTSLVRLAAKRQVVSLHYHDMNEVKGQSAAKRALEIAAAGGHSVIMSGP
                     PGTGKSMLASRFAGILPSMAEDEALEAAALQSLNGNYKLENWKRRPFRAPHHTASGVA
                     LVGGGGIPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLESGHITISRAARQADFP
                     AQFQLIAAMNPCPCGYLGHFNNKCRCTPDQVARYKAKISGPLLDRIDLHIEVPALKED
                     ELINTLQNESSDSVRARVEEARSRQLARQGKPNNLLGTKEIDQYCVADEAGLQLLKQA
                     ISRLSLSARAYHRILKVARTIADLANESAIKPAHIAEAIQYRRNDKA"
     gene            4912..8718
                     /gene="metH"
                     /locus_tag="PKL42_02135"
     CDS             4912..8718
                     /gene="metH"
                     /locus_tag="PKL42_02135"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNU65898.1"
                     /translation="MNFNQLSPVESQLRQLLEQRILILDGAMGTMIQQYKLTEADYRG
                     ERFADFKAPAGERELFVKGNNELLSLTQPQVIQEIHKKYLAAGADIIETNTFGATKVA
                     QDDYHMAHLVYEMNVQSAKLARAACDKYSTPDKPRFVAGALGPTPKTASISPDVNDPA
                     ARNVTFDELMNAYLEQVRSLVEGGADILMVETIFDTLNCKAALFAIEKFFEEHGSRLP
                     IMISGTVTDASGRILSGQTVTAFWHSVRHAKPLTIGLNCALGATLMRPYAEELSKIAD
                     TFVCIYPNAGLPNPMSDTGFDETPDVTSSLVKEFATSGFVNIAGGCCGTTPPHINAIY
                     EAIKDLPPRQVPTLAPITKLAGLEPFIIDENSLFVNVGERTNVTGSKAFARMIINEQY
                     EEALSVARQQVENGAQVIDINMDEGMLDAVKAMTHFLNLIASEPDIARVPIMIDSSKW
                     SVIEAGLKCVQGKSIVNSISMKEGEAEFLRQAKLCRRYGAAVIVMAFDEKGQADTYAR
                     KIEICKRAYDLLVAQDFPPEDIIFDPNIFAIATGIEEHNNYAVDFIEATRWIKQNLPH
                     AKISGGVSNVSFSFRGNDPAREAIHTVFLYHAIQAGMTMGIVNAGMMGVYDDLAPELK
                     ERVEDVVLNRITDPSNLLAPTERLIEIAGTLVAGGKKEAATLEWRGTPEAPVPVEKRL
                     AHAMVHGITEFIVQDTEEARAAVEASGGRPIHVIEGPLMDGMNVVGDLFGQGKMFLPQ
                     VVKSARVMKSAVAHLIPFIEAEKAAEQARTGVVAKPKGKVVIATVKGDVHDIGKNIVS
                     VVLQCNNFEVVNMGVMVPAAEILAMAKAENADIIGLSGLITPSLEEMAYVAKEMQRDP
                     HFHNAKTPLLIGGATTSRAHTAVKIAPHYDGPVIYVPDASRSVSVMQNLLTPESRGAY
                     LAEIQADYAKARSQHANKKGTPLLTIAEARANKAKLSFTGEFAPVKPKFIGRRVFKNI
                     DLSLIANYIDWSPYFQTWDLAGSYPAILTDKVVGEAATKVFNEAQVMLKKIIDGRWLT
                     ANGVIALMPANTVNDDDIEIYTDETRNQVAFTYYGMRQQTVKPVIDGVPRPNQCLADF
                     VAPTAQIGEPKVDDYVGLFAVTAGLGMEKRLAQFEAAHDDYSAIMFKSLADRLAEAFA
                     EYMHERIRTDFWGYASNEKLDAEALIKEQYQGIRPAPGYPACPDHTVKTDMFKLLQCD
                     EIDMQLTDSFAMMPAAAVSGFYFAHKEAKYFSVDKIGEDQLADMAKRRKLDKAYLERW
                     LAPNLS"
     gene            8736..9311
                     /locus_tag="PKL42_02140"
     CDS             8736..9311
                     /locus_tag="PKL42_02140"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HNU65899.1"
                     /translation="MKQIYQFNITLMDTQPDIWRTIQVSENDSFWNLHVAIQDAMGWL
                     DYHLHEFNFVDNTSKNLIRIGMPDEEFGDASILLGWEVRIKTYFEKAGTIARYSYDFG
                     DGWEHEVKLEAILPANAAISYPACIAGERACPPEDCGGIPGFEELLETLQHGKASEKK
                     ELNQWLKNHAKNYFPYSPEAFDPRAVKFDDP"
     gene            complement(9392..10651)
                     /locus_tag="PKL42_02145"
     CDS             complement(9392..10651)
                     /locus_tag="PKL42_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNU65900.1"
                     /translation="MNQPLPVSALANTAEIFLGRQPILDHKKQIIAFELLFRSKASLD
                     DADVSDDLLATSTVIVNTLSQFGLEQVLGNKLGFLNVSASLLLSETVNLLPPERIVLE
                     ILEDVPINAQIIARCQQLKDMGFRLALDDFEYRSEYDALFPLIDYVKIDLTITPVAKL
                     PEIIKFLRKVTSAKLIAEKVEEEALFQQCKDLDFDAYQGFFFAKPTLLKGKKPQPHQT
                     TLMRVMGELLGDVNLGKLETMFKDTPSLTLGLLKLVNSVGIGGGRQKIESIRQAIVVL
                     GQKQLMRWVQLLLYATPDGSAGGTLMLQVANRARLMELVAKKIDHHQPNFSDQAFMVG
                     MLSLADVVMQTPLEQVLEEIGVSDDIKSAVLSHEGLLGELLSLSDVIEKGDFKAAELE
                     IEKLGISANDLLAIQLETMQWANQISN"
     gene            10925..12304
                     /gene="mpl"
                     /locus_tag="PKL42_02150"
     CDS             10925..12304
                     /gene="mpl"
                     /locus_tag="PKL42_02150"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HNU65901.1"
                     /translation="MHIHILGICGTFMGGIAVLAKAAGHKVTGSDANVYPPMSTQLEA
                     QGIALIEGFDPTQTKLNPDIYVVGNVVARGNALMEEILNKGLPYISGPQWLAENVLQG
                     KWVLAVAGTHGKTTTSSMLAWILEYAGLAPGFLIGGVPENFSVSARLPQVPVQDNKSI
                     SPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFEDLAAIEKQFHHLVR
                     TVPQLGLIVANGKEPSLQRVIEKGCWTPVEKFGTDDDWQAANPQGNGAFDVLFKGQLQ
                     GHVAWDLLGEHNRMNALASIAAARHAGVAIETSIAALTKFKNVKRRMETKGEVNKITV
                     YDDFAHHPTAIETTVAGLRAKVGSNRIIAVLEPRSNTMKLGVMKDALPASLKEADVVF
                     CYGANLGWDAAEAMKPIASKAKSYDDLSVMVQAIAEIAQPNDHILVMSNGGFGGVHQK
                     ILDALKLKF"
     gene            12316..13290
                     /locus_tag="PKL42_02155"
     CDS             12316..13290
                     /locus_tag="PKL42_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNU65902.1"
                     /translation="MDKPLISSHRLSAAKLKNISAISIAIWASVFIHVVILSIHFESD
                     IKKFADRLPTLEVLLVNAKTQTKPEKADVLAQANLDRGGNTDQDRKMKTPLPAIKQQK
                     AELTVKPTTEVKSGARAAKKAAEETQEEKRVTELEQKAQELLTQIKATKQVESESKQA
                     STAKESETGDQETPSKLNSSDMIAQAIEIDRLEASIDKKYDEYQKKPKRKFIGARAKE
                     YKYALYVDTWRQKVERVGNLNYPEAAKNLKLYGNLQLTVSIKSDGTLESVELTRSSGH
                     KVLDDAAKQIVELAAPYARFPDDIRNEVDILSITRTWTFTKEDSLATQ"
     gene            13340..13651
                     /locus_tag="PKL42_02160"
     CDS             13340..13651
                     /locus_tag="PKL42_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNU65903.1"
                     /translation="MTSHFQHHVFFCLNQRENHEACCADKGAEAAFDHMKARIKKLGL
                     NGEGKVRINRAGCLDRCNEGPVLVIYPQAIWYTFIDTEDIDEIIESHILNGKVVERLV
                     V"
     gene            complement(13805..14236)
                     /locus_tag="PKL42_02165"
     CDS             complement(13805..14236)
                     /locus_tag="PKL42_02165"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNU65904.1"
                     /translation="MTTCSLCQPTPFETLWADDFCRVVLLNDADYPAYCRVELLAHVK
                     EMSDLPPIARAKMMQVVFAVETAVREVILPDKINLASLGNKTPHVHWHVIPRFSHDKH
                     FPNSHWGEAVRESHMPQLNATQKQLLADKIKAHIDSAFSSN"
     gene            complement(14239..15336)
                     /gene="nadA"
                     /locus_tag="PKL42_02170"
     CDS             complement(14239..15336)
                     /gene="nadA"
                     /locus_tag="PKL42_02170"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNU65905.1"
                     /translation="MQTSTIKFEKFQAADDADCQRRIIAAREKLGKRLVILGHHYQNE
                     SVYRHADYTGDSLKLSRYCESLDAEFIVFLGVHFMAEVADILSRPEQIVILPDLAAGC
                     SMADMANLAKVERSYRELNKVLNFDETITPITYINSAADLKAFCGEHGGIVCTSTNAP
                     KILEWGFKQREKVLFFPDQNLGRWSGHKMGIPLEQMPVWDPDQPLGGLTVEQIRNAKI
                     LLWKGHCAVHQMFRPQNITQFKAQHPDGLVISHPEAPFEVCLNSDYVGSTEFILKTVT
                     EAPANSKWLVGTELNLVNRLANQMKPQNKLVQFMSHVVCECSTMARIDPQHLAWVLEN
                     LADGKVVNQIKVPDRVAKLAKLTLDRMLAAS"
     gene            complement(15380..15832)
                     /gene="nudB"
                     /locus_tag="PKL42_02175"
     CDS             complement(15380..15832)
                     /gene="nudB"
                     /locus_tag="PKL42_02175"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HNU65906.1"
                     /translation="MKKFKIPVSALVLIHTADFQVLVMERADKAGFWQSVTGSVEGDE
                     TPFQAAIREVKEETGLNALDYDFQDWQASNVYEIYPHWRHRYAPGVVENREHLFGLQL
                     HSPLPIQLAPDEHVRYEWVDWREAAQRVFSWTNVDALKRLGERHQRTL"
     gene            complement(15927..16880)
                     /gene="ispH"
                     /locus_tag="PKL42_02180"
                     /gene_synonym="lytB"
     CDS             complement(15927..16880)
                     /gene="ispH"
                     /locus_tag="PKL42_02180"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNU65907.1"
                     /translation="MANTQADILLANPRGFCAGVDRAIIIVEQALEKFGAPIYVRNEV
                     VHNKFVVDELRKKGAIFVNELDKIPAGSTVIFSAHGVSKAVRLEAEARGLTAYDATCP
                     LVTKVHIEVEKMRKAGLEIIMIGHKGHPEVEGTMGQAEQGMYLVETPDDVLALQVINP
                     EKLAYVTQTTLSMDDAAKVIDALQARFPAIRAPKSDDICYATQNRQDAVKIMAKDCDL
                     VIIVGSPNSSNSNRLREVAQNQGIEAYMVDNASHLNPEWLTGKKKIGVSAGASAPEIL
                     VKDVISKLQSLGAANVQELQGVVENVIFQLPKNLTQASKKI"
     gene            17096..17965
                     /gene="purU"
                     /locus_tag="PKL42_02185"
     CDS             17096..17965
                     /gene="purU"
                     /locus_tag="PKL42_02185"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HNU65908.1"
                     /translation="MNNSAVKNTATLLITCPDSKGLVAAIAEFLYQHNANILHADQHQ
                     DAENNLFLMRVEWDLTGFSMPIADFEQHFTAIAVRFNMEWQLKLSNKPVRVAIMVSQY
                     DHCLADLLHRHKNGELDCEIPLIISNHRDTESLANFYGVDFHYIPVTKDNKAAAEAQQ
                     FALFEQYAIDLIVLARYMQILSPDFVARYPKQIINIHHSFLPAFIGARPYHRAFERGV
                     KLIGATSHYVTEVLDEGPIIEQDIDRISHRDQVEDLIQKGRDLERIVLSKAVRWHIEN
                     RILLYANKTVIFD"
     gene            complement(18094..19344)
                     /gene="lysA"
                     /locus_tag="PKL42_02190"
     CDS             complement(18094..19344)
                     /gene="lysA"
                     /locus_tag="PKL42_02190"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNU65909.1"
                     /translation="MNTFTLKNNTLFAENVALTDIAKRFSTPCYVYSKTALIHAFESF
                     NAGFKGSNHLVCFAVKSNPNLAILNLFASLGAGFDIVSGGELARVLAAGGDPKKIVFS
                     GVGKTAVEMQAALNAGIFCFNVESRSELVRLNTVAGAMGKVAPVSLRVNPNVDAKTHP
                     YISTGLKNNKFGVAYEDALNIYLEAANMPNISIHGVDCHIGSQITELSPFLDAFDRVL
                     TLVDALEQHGIHIQHIDAGGGIGICYSDETPPEFSAYAAAMQEKLGQRQVKLVFEPGR
                     ALVGNAGILLTQVEYLKPTESKNFAIVDAAMNDLMRPALYDAYHEIQAVTPRNTPAIS
                     YEIVGPVCESGDFLGHDRTLALEEGDLLAIMSAGAYGMSMASNYNTRPRAAEVMVDGE
                     TCHLIRKRETVADLFAQENTLPKV"
     gene            complement(19394..19516)
                     /locus_tag="PKL42_02195"
     CDS             complement(19394..19516)
                     /locus_tag="PKL42_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HNU65910.1"
                     /translation="MHKNIAYLLLLAALCAVASGCGTKGPLYIPEQQYPQPAEK"
     gene            19729..20178
                     /locus_tag="PKL42_02200"
     CDS             19729..20178
                     /locus_tag="PKL42_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HNU65911.1"
                     /translation="MKLTVQQLNTPIANLPRKAQQGFTLIELMIVVAIIGILASIAIP
                     AYSDYVKRGKAAEATSTLANLRVQIEQYYQDNRTYVGFACNPTQKYFTYSCTTQTATT
                     YTLNAAGNSAQDMGSFEFTLNQANARTSKFDGTTGATCWLTSKGGSC"
     gene            20172..20753
                     /locus_tag="PKL42_02205"
     CDS             20172..20753
                     /locus_tag="PKL42_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HNU65912.1"
                     /translation="MLVLAKNRGKTSHRGFTLIELMTVVAVIGVLAALGLPSYRIWIE
                     NTKIRTAAESIQSGLQKARVEAIKRNVRVQFVLGANSTWTVSCVTAAQCADLAGGVVE
                     TRGASDGSSANITVTATPAGSTTIIFSNFGTVSPASAIAPVVPVPFTQLELNNAALDS
                     ADSRKLRVMIGPGGVGRTCDPYSGLSTSDPRKC"
     gene            20810..21193
                     /locus_tag="PKL42_02210"
     CDS             20810..21193
                     /locus_tag="PKL42_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNU65913.1"
                     /translation="MVKKSQRGIALLESLIGILIFSMGILAIAGLQGFMIKGTTEAKY
                     RSDASVIAQRRVAMMWADAKANPAALSAAYSEVNTAIPDLPNGRRTTTFVAAIPDAVT
                     VTVTWQTPGEPQHNYSTNARITGAR"
     gene            21141..>21650
                     /locus_tag="PKL42_02215"
     CDS             21141..>21650
                     /locus_tag="PKL42_02215"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNU65914.1"
                     /translation="MSRSIIIQQTPELLGQDNMRFIQHHQSAKSFRQAGFSLVELMVG
                     LVIGLLATLAIMQIFSAFEGQKRTTSGTADAQTSGSVALYTLQRDVQLAGFGLPLYDE
                     ANMPLKCNAPSYPVVDHDNNAATPNINMSAVDIINGATATDSDIVIVRYGASASGGVP
                     VPVVGAPNAA"
BASE COUNT         6239 a         4866 c         4893 g         5652 t
ORIGIN      
        1 agttagttac agccaagatt aaaaaccagc gatcaaccac ggattacgga tacccgtaat
       61 tttgaatatt acacaattat tcaagaactc tatatttgtg aaaggtgaaa ggtttttttc
      121 tgcaaaatta aagtgatatt gcgcttaaaa acggacagcg aaatgtagtt taatacgcca
      181 ctaaattaca cgagaaaaaa ataatgctct gcgagccttt gtttataagg cttccagagc
      241 atcgaaaaag gttggtcatt aaaacacaca aaaaacgggg ctttttaccc cgtttttgct
      301 ttaacccaaa ttgtggatga cttgcttaaa tgttataagc gcgctcaccg tgtgaggtgg
      361 tatccaagcc ttcacgttct gcttcttctg atacacgtaa tccaacaatc aaatcggcta
      421 ttttataagc gatgaatgct gcaatacctg accaaatgat agtgatgatg acaccctcgc
      481 cttgaatcaa gacttgtgag gctattgaga aatcagcttt accagtgcca cccaaacttg
      541 gtgcagtaaa tacgccagtt aataatgcac ccacaatacc acctacaccg tgcacaccaa
      601 atacgtcgag cgagtcatcg gcccccagta ttttcttgag gccagtcacc ccccataggc
      661 acaagaagcc gccagcaaaa ccaatgataa tgccacccat tgggccaact gaaccgcatg
      721 caggtgtaat tgctaccaaa cctgccacag caccagatgc agcacccagc atagacgcct
      781 tgcctttagt gaaggcttca ccaattgacc aggccaatac tgcagcagcc gttgcaaata
      841 gtgtgttgac tagcgctaag cctgcgccac cggttgcttc caagttagag cctgcgttaa
      901 aaccaaacca gcccacccat aacaatgcag cacccagcat ggttaacgtt aagttatgag
      961 gcgttaatgc ttctttgcca taaccaatac gtttgccaac catgtaagca cctactaaac
     1021 cagcaaccgc agcattgata tgcacaacgg taccgccagc aaagtctaat gcgccttgac
     1081 caagcaagta gccacccgca ccccaaacca tatgggcgat aggaatgtag ctcaatgtga
     1141 accaaatcgc tgagaataat aataccgcac taaatttaat acgttcagca aaagaaccca
     1201 caatcaatgt agccgttaaa cccgcaaaaa tgcattgaaa agcaatgaag acaaactctg
     1261 gaatcttaac gcctgcagta aaagtgtcag acagcgaatc tgaggtaacc cctgccaaga
     1321 aaagtttacc aaagtcaccg atgatggggc ctgacccgct accaaatgcc aagctatatc
     1381 cataaacagc ccacaataca gaaacaagtg agaacacaac aaacacttgc atcaagactg
     1441 atagcatgtt tttactgcgc actaatccac cgtaaaacaa agctaaacct ggcaccatta
     1501 acacaaccag aatagttgaa atcatcatcc aagtggtgtc gcctttgtct accgttggcg
     1561 ccacctctgg cacggccgcc tccgccggca acgcttcggg ggctactgtt tcaactggtg
     1621 ctgcctcagc gggtgttgcc accatttctg ttggtgcagc atctaccact ggggcttctt
     1681 ctgccaatac atttcccgtt aaaccaaacc ctagcatggt ggctaatgcc aatgtcgaaa
     1741 gaattttttt catgttgatc cccttagagt gcttctgagc cagtttcacc ggtacgaata
     1801 cgggtgactt gctctaaatt aaacacaaat attttgccgt caccaatttt tccagtagcg
     1861 gcagattttt caatcgcttc aacgacttgg tctaaaattt tgtcatcaat ggcaatttct
     1921 acttttactt ttggtaaaaa atctaccacg tactcagcac cacgataaag ttcagtatgg
     1981 cctttttggc gaccaaaccc ttttacttct gtcacagtga tgccttgtac accaatttct
     2041 gaaagtgctt cgcgcacttc atcaagctta aagggcttga taattgcaga tataaatttc
     2101 atgagtgcct cctaaaagtg cgagaaaact cgcactaacg attaaaaagt tttttgtaca
     2161 aatacagtaa actgctcttt agcaatattt tggccagaag catcggtgaa atcagatttc
     2221 ttgccgtcgg tatcggtgaa gtatgcacct atattgatgc cgctttccca tgatttcgtg
     2281 acgcctaatt tccaatcagc ataatcaaac tggctattgc ctttgccggt aaaatcctgg
     2341 tagccaacat gcgctacgcc agaaaatccg ctaccaaatg gcactgccaa atttaactct
     2401 gtataagtag agccatcggc attatcaaaa ccaaatgcgc catctgatac aaccgcagaa
     2461 accttaccag aaagccatcc gtacgaaagg ctgccgtaaa tttcagtggt ttcagctttt
     2521 tttgtgccgc taaattttct accagggtaa atgtattgca atagtcctac gttgtagcca
     2581 atacctgttt cgccaatcgt gttagcgtag ccaccgtata ggtctaactc caggctcgag
     2641 cttttgtagt aatcgccatc cgttaaccag ctaatgtttg aagcccaagc acccgcataa
     2701 aaaccgctgg catgggtata atcaacacca ccctgaatag ctgggtcttc acgtgtttgt
     2761 gtaaggccgc gaaaaatgta ctggctatat aaaccaacgt tgtaagcaaa agcatgtggt
     2821 gaagcaggag ctgggacgac ggtcgcctca ggtgcggctt catctgcatg tgaaattgca
     2881 ggtaatgtaa atacgcttag aacggctagt gataataatg aattacgcat attgcttccc
     2941 cttgagaacg ttgtaaaaat aaaattgact cactacactc taagcaaggg ctgtgccaat
     3001 ataaattttg attaaaatca aacacttata atatttacct gtacggacag atttatctaa
     3061 atagattaaa acctgataaa ctacgcacta aatacaaagg aatcattatg ctaagttcag
     3121 ataaattaaa tgaattatca ttaaaaatca aagagataat tagcagctca cctttggggg
     3181 atgctgaaaa aaacattcat gcactagtta agggcgcgct caccaaaatg gaactcgtca
     3241 cgcgtgaaga atttgacgtg caagccgagg tgctaagaag caccaaagaa aaacttagcc
     3301 agttagaggc aaaattagct gaattagaag ctcagcaaaa aaaatagcat gagtttagcc
     3361 gttctatata gccgtgcttt aagtggtatg gatgcccctg aagtggtggt tgaggtgcat
     3421 ttagccaacg ggctacccag ctttaccatc gttggcctcc ctgaagctga ggtaaaagag
     3481 agtaaagacc gtgtgcgtgc agcattgcaa accgcacagt ttgagtttcc tgcccgacgc
     3541 attacggtga atttagcccc tgccgactta cccaaagaaa gtgggcgcta tgatttgcca
     3601 attgctttgg gtattttggc agcctcaggg caaattccaa ccgaagcatt atctcgctat
     3661 gagtttgctg gagaattggc gctaacaggt gaactcagac caattagagg cgcactggca
     3721 atggcattta aatcttataa aagcgggcgc gcttttatat tgccgcacga aagtgcagca
     3781 gaagcagcct tggttgcaga cgcgatagtc tacccagcca ataccttgct ggaagtatgc
     3841 gcgcatttta ccggccgcac atctttggtg cggttggccg ccaagcgtca ggtggtcagc
     3901 ctacattatc atgacatgaa tgaggtcaaa gggcagtctg ctgctaagcg cgcattggaa
     3961 attgcggcgg ctggaggcca tagcgtcatt atgagcggac cgccaggcac aggtaaaagc
     4021 atgctggcaa gccgctttgc tggcatattg ccgagcatgg cagaagatga ggcgctagaa
     4081 gccgcagcac tacagtcttt gaacggtaac tataagttag aaaattggaa acgccgccca
     4141 tttagagcgc cacatcacac agcatcaggt gttgcactgg tgggtggcgg tggaataccc
     4201 aggccaggag aaatttcact cgcgcatcat ggcgtattat ttctagatga attaccagaa
     4261 tttgaccgca aagtgcttga agtattacgt gagccgttag agagtggaca tatcaccatt
     4321 agccgcgccg cgcgtcaagc tgattttcct gcgcaatttc aactgattgc tgccatgaac
     4381 ccgtgccctt gtggatatct cggacatttt aataataagt gccgctgtac acctgaccaa
     4441 gttgcgcggt acaaagctaa aatatcaggg ccattactag accgtattga tttacatatt
     4501 gaagtccccg cacttaaaga agatgagtta attaacacgc tacagaatga atcttcagat
     4561 tcggtgagag caagagttga agaagcgcgt agcaggcaac ttgctagaca aggcaagccc
     4621 aataatttat tgggtacaaa agaaattgat cagtattgcg tggcggatga ggcagggttg
     4681 caactattaa aacaggctat cagtcgttta agtttgtctg ctcgggctta tcatcgtatt
     4741 ttaaaagttg ccagaactat tgcagattta gccaatgaaa gtgccattaa accagctcat
     4801 attgctgagg caatacaata ccgccgcaac gacaaggcat aatttacaac taactttact
     4861 gaataataga cttttttatt aagtagaatt aaatcaacaa aacgagtaaa aatgaatttc
     4921 aatcaattat ccccagttga atcccaattg cgccagttat tagaacagcg catcttaata
     4981 cttgatggcg cgatgggcac gatgattcag caatacaaac tgaccgaagc cgattatcgc
     5041 ggtgagcgct ttgccgattt taaagcgcca gcgggcgagc gtgaactgtt tgtaaaaggc
     5101 aacaacgagt tactaagcct gactcagcct caggtgattc aagaaattca caaaaaatat
     5161 ctagctgcgg gtgcggatat tattgaaacc aacacctttg gggccactaa agttgcgcaa
     5221 gatgattacc acatggcgca tttggtgtat gaaatgaacg tacaatcggc caagcttgcc
     5281 cgcgccgctt gcgataaata cagcacaccc gataaaccac gatttgttgc gggcgcgcta
     5341 ggcccaacgc ctaaaacggc ttctatttcc cctgatgtga acgaccccgc cgcgcgaaat
     5401 gtcacgttcg atgaactcat gaacgcctat ttagagcaag tacgcagctt ggtagaaggc
     5461 ggtgcagaca ttttaatggt ggaaaccatt tttgatacgc tcaactgcaa ggcggcctta
     5521 tttgccatcg aaaagttctt tgaagaacat ggtagccgtc tgccgattat gatttcaggc
     5581 actgtgacgg atgcttctgg gcgtattttg tccggtcaaa ccgtcacggc attctggcat
     5641 tcggtacgtc atgccaagcc gcttaccatt ggtttaaact gcgcattggg cgcaacctta
     5701 atgcgccctt acgctgaaga gctttcaaaa atcgccgata cttttgtgtg catttacccc
     5761 aatgcaggtc tacctaaccc aatgagcgac acgggctttg acgaaacgcc cgatgtgact
     5821 tcaagcctag tgaaagagtt cgccaccagc ggctttgtga atattgcggg cggttgttgt
     5881 ggcactacgc caccacacat taacgcgata tacgaagcga ttaaagattt accacctcgc
     5941 caagtgccga cccttgcacc aatcaccaag cttgcaggtt tagagccatt tatcattgat
     6001 gaaaattcac tctttgtaaa cgtaggcgag cgcaccaatg taacgggcag taaagccttt
     6061 gcccgcatga ttatcaacga gcaatatgaa gaagctttga gcgtagcgcg ccagcaagta
     6121 gaaaatggcg ctcaagtcat tgacatcaac atggatgaag gcatgttaga tgccgttaaa
     6181 gccatgacgc actttttaaa tttgattgcc agcgagcctg atattgcccg cgtgccgatt
     6241 atgattgatt ccagtaaatg gtcggtgatt gaagcgggct tgaagtgcgt acaaggtaaa
     6301 tccatcgtca actctatctc gatgaaagaa ggtgaagcag agtttttacg tcaagccaaa
     6361 ctttgccgcc gttacggcgc ggctgtgatt gtgatggcgt ttgatgaaaa aggccaagcc
     6421 gacacttacg cgcgtaaaat tgaaatttgt aaacgcgctt atgatttatt ggtcgcgcaa
     6481 gactttccgc cagaagacat catttttgat ccaaatattt tcgccattgc cacaggcatt
     6541 gaagaacaca acaactacgc ggtagatttt attgaggcca cgcgctggat taagcaaaac
     6601 ttaccgcacg ccaaaatttc aggcggtgtt tcaaatgtga gttttagctt tagaggcaac
     6661 gaccccgcgc gtgaagcgat tcacaccgtg tttttatacc acgcaattca ggcgggcatg
     6721 accatgggca ttgtaaacgc aggcatgatg ggcgtgtacg atgacttagc gccagagcta
     6781 aaagagcgtg tagaagatgt ggtgctaaac cgcattaccg accccagcaa tttacttgcg
     6841 ccgactgaac gcctgattga aattgctggc acgctggtag caggcggcaa aaaagaagct
     6901 gcgactttag agtggcgggg cacgccagaa gcaccggtgc cagttgaaaa acgcttggcg
     6961 cacgccatgg tgcatggcat tactgagttt attgtgcaag ataccgaaga agcccgtgca
     7021 gcagttgaag ccagcggtgg cagacccatt cacgtgattg aaggcccgct catggacggc
     7081 atgaatgtgg tgggagattt atttgggcaa ggaaaaatgt ttttgccgca agtggtgaaa
     7141 tctgcccgcg tgatgaaatc tgctgtagcc cacttaatcc cctttattga agccgaaaaa
     7201 gctgcagagc aggcgcgtac tggcgtagtt gccaagccaa aaggcaaagt ggtgattgcc
     7261 acggtgaagg gcgacgtgca tgacatcggc aaaaacattg tgtctgtggt gttgcaatgc
     7321 aacaactttg aagtggtgaa catgggcgtg atggtgccag cggcggaaat tttagccatg
     7381 gcaaaggctg agaatgccga cattattggt ttgagcggct taattacgcc atcgttagaa
     7441 gaaatggcct atgttgccaa agaaatgcaa cgcgacccgc attttcacaa cgcaaaaacg
     7501 ccattactga ttggtggcgc aacgacttca cgcgcgcata cggcggtaaa aatcgcgccc
     7561 cattacgatg gcccagtcat ttacgtgcct gatgcttcac gttcagtatc cgtgatgcaa
     7621 aatttgctta cgccagaaag ccgtggtgcc tatttagcag agattcaagc cgattatgcc
     7681 aaagcgcgta gtcagcatgc caataaaaaa ggcacgccgc tactcactat tgcagaagct
     7741 cgtgccaata aggccaaact gtcatttacg ggtgaatttg cacctgtgaa gccaaagttt
     7801 attggccgtc gtgtgtttaa aaacattgat ttaagcctaa tcgccaatta cattgattgg
     7861 tctccctatt tccaaacatg ggatttagct ggcagttacc cagccatttt gaccgataaa
     7921 gtagtcggcg aagcggccac taaagtcttt aatgaagcac aagtgatgct gaaaaaaatc
     7981 attgatggac gctggctcac cgcaaatggc gtgattgcgc tcatgcctgc caacacggta
     8041 aatgatgacg atattgaaat ttacacagac gaaacgcgca accaagtggc gtttacctat
     8101 tacggaatgc gccagcaaac cgtaaaaccc gtgatagacg gtgtgccacg cccaaaccaa
     8161 tgtttggcag attttgttgc gccgacagcc caaataggcg aaccaaaggt tgacgattat
     8221 gttggtttat ttgctgtaac cgctggctta ggcatggaaa aacgactagc ccaatttgaa
     8281 gccgcgcacg acgactacag cgcaattatg tttaaatcac tcgctgaccg ccttgcagaa
     8341 gcctttgcag aatacatgca cgaaagaatc cgcactgatt tttggggtta tgcaagcaat
     8401 gaaaaattag atgccgaagc cttaattaaa gagcaatatc aaggcattag accagcccca
     8461 ggctaccccg cctgcccaga ccataccgtt aaaactgata tgttcaagct attacaatgt
     8521 gatgaaattg atatgcaact caccgattca ttcgccatga tgcctgccgc ggcagtttca
     8581 ggcttttact ttgctcacaa agaggctaaa tattttagtg tagataaaat tggtgaagac
     8641 caattggcgg atatggcaaa acgcagaaaa ttggataaag cttatttaga gcggtggcta
     8701 gcgccgaatt taagttaaaa aagagggcgt tggccatgaa gcaaatttat caatttaata
     8761 ttacactaat ggacacacaa ccagatatat ggcgcaccat tcaagtgtct gaaaatgata
     8821 gcttttggaa tttacacgtg gcgattcaag atgccatggg gtggctagat tatcacttac
     8881 atgaatttaa ttttgttgat aatacaagca aaaacttaat acgcattggc atgccagatg
     8941 aagaatttgg cgatgccagt attttgttag gttgggaagt gcgcattaaa acgtactttg
     9001 aaaaagcagg cacaattgcg cgatattcgt atgattttgg cgacggctgg gagcatgagg
     9061 ttaaacttga agctattctg ccagctaatg ccgctattag ttaccctgca tgcattgctg
     9121 gagaacgcgc ttgcccgcca gaagactgcg gcggcatacc tggctttgaa gaacttttag
     9181 aaaccctaca gcatggtaaa gccagtgaga aaaaagagct taatcaatgg cttaaaaatc
     9241 acgcaaaaaa ttactttccc tactcaccag aagcatttga cccacgtgca gttaaatttg
     9301 acgaccctta attacgttgg gcaaatgcct ttagtgcaaa caaataaata aatagtatga
     9361 cggtgagcgt atgctattta agggttaact tctaattact aatttgattt gcccactgca
     9421 tagtttcaag ctgaattgcg agtaaatcat tggcgctgat gcctagtttt tcaatttcaa
     9481 gttctgcggc tttgaaatca cctttttcaa tcacgtcaga taaagataat agttcgccta
     9541 gcaagccttc atggcttaac acggcgcttt taatgtcatc tgatacgcca atttcttcta
     9601 gcacttgttc tagcggtgtt tgcatcacaa catcggcgag cgataacatg cccaccataa
     9661 acgcttggtc gctaaagttt ggttggtgat ggtctatttt cttggcaacc aattccatta
     9721 agcgtgcgcg gttggctact tgtagcatga gtgtgccacc agcactgcca tcaggggttg
     9781 cgtagaggag cagttgtacc cagcgcatga gttgtttttg gccgagcacg acaatggctt
     9841 ggcgaatgga ttcaattttt tgtctaccgc cgccaatgcc cacagaattc actagtttca
     9901 ataaccccag tgtgagactg ggggtatctt taaacatggt ttctaattta ccaaggttaa
     9961 catcacctag caattcgccc atcacgcgca tgagtgttgt ttgatgaggc tgcggttttt
    10021 tacctttcag caaggttggt ttggcgaaaa agaagccttg gtaagcgtca aaatctaaat
    10081 ctttacattg ttgaaatagt gcttcttctt caactttttc agcaatcagt ttggcgctgg
    10141 tgactttacg taaaaacttg atgatttcag gtaacttagc tactggtgtg atagttaaat
    10201 caattttgac gtaatcaatg agtggaaata gtgcgtcata ttctgagcgg tattcaaaat
    10261 catcaagggc aagtctgaac cccatgtctt ttagttgctg gcagcgtgca atgatttgtg
    10321 cgttaattgg cacgtcttca agtatttcta acacaatacg ctcaggtggc agcaagttaa
    10381 cggtttcact gagtagcaag ctggctgaaa catttaagaa cccaagtttg ttacccagca
    10441 cctgttctag gccaaattga ctcagcgtgt ttacaattac cgtagaggtg gcgagcaggt
    10501 catcactgac atccgcatcg tctaaactgg cttttgatct aaacagcagt tcaaaggcaa
    10561 tgatttgttt tttatgatct aaaatgggct gacgacccaa aaaaatctct gcggtattcg
    10621 ccaaggctga tacaggaaga ggttggttca taatttttta ctccgtttgg tttaatgcac
    10681 tctgccattt gtggcaagga tggcttatca tacgacgtgt ggattaatat caatcgcgtc
    10741 gttaagcgct taagtgatta tcggcaagca ggtttttttc tttagtgttt tttcagtttt
    10801 ttcatcaaaa aaaagtacgt ttaagaaaaa attattttga atgaaagata tgacgcaaaa
    10861 aaattgccat aatccaacac atttattaaa ttaagcgctt gttataaaag gttaaatttt
    10921 aaagatgcat attcacatac tgggtatttg cggcacattt atggggggca ttgcggtgct
    10981 ggcaaaagcc gctggtcaca aggtgacagg cagtgatgcc aatgtttacc cgccgatgag
    11041 tacccagctc gaagcgcaag gcattgcgtt gattgaaggt tttgacccaa cgcagacaaa
    11101 attaaacccc gatatttatg tggtgggcaa tgtcgtggcg cgcggtaacg cgctgatgga
    11161 agaaattcta aataaaggcc tgccgtatat ttctggcccg caatggctgg cggagaacgt
    11221 gctacaaggc aagtgggtgc tggcggtggc gggtacacat ggtaaaacca ccacttcatc
    11281 catgttggct tggatattag agtatgcagg cttggcgccg ggctttttaa ttggcggtgt
    11341 gcctgagaat ttttcggttt ctgcaaggtt accgcaagtt cccgtgcaag ataacaaaag
    11401 catctcgccc ttttttgtga ttgaagccga tgaatacgac acagcatttt ttgataaacg
    11461 ctcaaaattt gtgcattacc gtccacgcac tgcggtattg aataatctgg agtttgacca
    11521 cgcagatatt tttgaagact tggcggcgat tgagaagcaa tttcaccact tggttagaac
    11581 ggtgcctcag cttggtttga tcgtggctaa tggtaaagaa ccaagcttac agcgcgtgat
    11641 tgaaaaaggc tgttggacgc ctgtggaaaa atttggcacg gatgacgact ggcaagcagc
    11701 gaatccgcaa ggcaacggcg catttgatgt gttatttaaa ggtcagttac aagggcatgt
    11761 tgcatgggat ttgcttggtg agcataaccg catgaatgca cttgccagca ttgccgcagc
    11821 gcgccacgcc ggggtagcca ttgagacgag cattgcagca cttaccaaat ttaaaaatgt
    11881 aaaacgccgt atggaaacca aaggcgaggt gaacaaaatc acggtgtacg atgattttgc
    11941 gcaccaccca acggcgattg aaactactgt ggcaggtttg cgcgccaaag tgggcagtaa
    12001 tcgcattatc gcggtgttag agccacgctc aaacaccatg aaattgggcg tgatgaaaga
    12061 cgcgctgccg gccagcctaa aagaagctga tgtggtgttt tgctatggtg caaatttagg
    12121 ttgggatgct gctgaagcaa tgaaaccgat agctagtaaa gcgaaaagct acgatgattt
    12181 gagcgtgatg gtgcaggcaa ttgcagaaat tgcccagcca aatgatcata ttctggtgat
    12241 gagtaacggc ggttttggcg gcgtgcatca gaaaattctg gatgctttaa aattaaaatt
    12301 ttaggtcaat agattttgga taaaccttta atttcttctc accgattatc agctgctaaa
    12361 ctaaaaaaca ttagcgcgat aagcatcgcc atttgggcct cggtctttat tcatgtggtg
    12421 atattaagca ttcattttga gtctgacatt aaaaagtttg ccgaccgttt gccaacgctg
    12481 gaagtgttgc tggtaaatgc taaaacacaa acaaaacccg aaaaagcgga tgtattagcg
    12541 caggccaatt tagatcgtgg tggcaatact gaccaagatc gaaaaatgaa aacgccgttg
    12601 ccagccatta agcagcaaaa agccgagctg acggttaagc caaccactga agtaaaatcg
    12661 ggtgcgcgtg ccgctaaaaa agccgctgag gaaacccaag aagaaaaacg cgtgaccgag
    12721 cttgagcaaa aagcgcaaga attgctgacg caaatcaagg cgactaaaca ggtcgagtcc
    12781 gaatctaaac aagcttctac cgcaaaagaa tcagaaacgg gtgaccaaga aacgccgagc
    12841 aaactcaact catccgatat gattgcgcaa gccatcgaaa tagataggct tgaggctagt
    12901 attgataaaa agtatgatga atatcagaaa aagccaaaac gtaaatttat tggggcgcga
    12961 gccaaagaat ataaatatgc actttatgta gatacatggc ggcaaaaggt ggaacgtgtt
    13021 ggcaacctca attacccaga agccgctaaa aatctcaagc tttacggaaa cctacaactc
    13081 accgtttcaa ttaaatctga tggtacgcta gagtccgtag aactcacgcg cagttctggc
    13141 cacaaagtgc tggatgacgc cgccaaacaa attgttgaac ttgccgcgcc ttacgcacgc
    13201 tttcctgatg atattcgcaa tgaagtcgat attttaagca ttacgcgcac ctggacattt
    13261 accaaagaag acagcttagc gacccagtaa gggtatgtgg acagtattgg ctaaacttta
    13321 aagtaggata aataaaccga tgaccagcca ttttcaacat cacgtatttt tttgcctcaa
    13381 ccaacgtgaa aatcatgagg cctgctgtgc agataaaggc gcagaagctg cttttgacca
    13441 catgaaagcg cgtattaaaa agctaggcct caatggtgaa ggcaaagtgc gtatcaatcg
    13501 cgcaggttgt ttggaccgtt gcaatgaggg gccagttttg gtgatttatc cccaagccat
    13561 ttggtatact tttattgata ctgaagatat tgatgagatt atcgaaagcc atatactcaa
    13621 cggtaaagtg gttgagcggt tggtggtata gactgaaaaa aacaccttaa aattacatat
    13681 ttaaaacggc tttaaaacag caccttttgc gccgagaaaa aatttatgct ctgcgagcct
    13741 ttatttataa ggcttccaga gcatcgaaaa aggttgtggc taaaaaagcc tgttttttgg
    13801 atgattaatt ggatgaaaaa gcgctgtcaa tatgcgcttt tattttatcg gccagcaatt
    13861 gtttttgcgt ggcattgagt tgcggcatat gactttcacg cacggcttca ccccagtgcg
    13921 aatttggaaa atgcttgtca tgcgaaaaac gcggaatcac atgccaatgt acatgcggcg
    13981 ttttgttgcc caagctcgcg aggttaattt tatcgggcag aatcacttca cgcactgcgg
    14041 tttcaaccgc aaacaccact tgcatcatct ttgcccgcgc aattgggggt aaatctgaca
    14101 tttctttcac gtgtgcaagc aactctacgc ggcaataggc ggggtaatct gcgtcgttca
    14161 gcaacaccac gcggcaaaaa tcatccgccc ataatgtctc aaaaggcgtt ggctggcaaa
    14221 gggaacaagt cgtcaatttt aggatgccgc taacatgcgg tccagcgtga gttttgctag
    14281 ttttgccaca cggtcaggca ctttgatttg gtttaccact ttgccatcgg ctaagttttc
    14341 tagtacccac gccaaatgtt gcgggtcgat gcgcgccatg gtggagcatt cgcacaccac
    14401 atggctcata aattgcacta gcttgttttg cggtttcatt tggttggcaa ggcggttgac
    14461 taagttgagc tcggtgccga ccagccattt gctgttggca ggcgcctcgg taaccgtttt
    14521 tagaataaat tccgttgaac ccacataatc tgaatttaaa catacttcaa acggcgcttc
    14581 tgggtgcgaa atcaccaagc catcaggatg ttgtgcctta aattgcgtga tattttgcgg
    14641 gcggaacatt tgatgcaccg cacaatggcc tttccacagc aagatttttg catttctaat
    14701 ctgctcaacc gttaagccgc caagtggctg gtcagggtcc caaacaggca tctgctcgag
    14761 cggaatcccc attttatgtc cgctccagcg gcctaagttt tggtctggaa agaacaagac
    14821 tttttcgcgc tgcttaaagc cccactctaa aattttaggg gcattggtgc tggtgcagac
    14881 gatgccgccg tgttcgccac aaaaagcttt aaggtcggca gcagagttga tgtaggtaat
    14941 gggcgtgatg gtttcatcaa aattcagcac tttattaagc tcgcgataac tgcgctcaac
    15001 cttggctaag ttggccatgt cggccattga gcagcctgcg gcaaggtcgg gcaaaatcac
    15061 aatttgctca ggacggctta atatatcggc cacttccgcc ataaagtgca cgcctaaaaa
    15121 cacaataaat tcagcatcta agctttcaca ataacgtgaa agcttgagcg agtcgcccgt
    15181 gtaatcggca tgacgataaa cgctttcatt ttgatagtga tgaccgagaa tgacaaggcg
    15241 tttgcccaat ttttcgcgcg cagcaataat tcggcgctgg caatcggcat catctgccgc
    15301 ctgaaatttt tcaaatttaa tggttgaagt ttgcatagtg ttgttttaaa ccacttaaat
    15361 atttaaacaa ggctttattt tacagcgtgc gttgatgtct ttcacctaat cttttcagtg
    15421 catcaacatt agtccaagaa aaaacgcgtt gcgcagcttc gcgccaatcc acccattcgt
    15481 aacgcacatg ttcgtctggt gctaattgga taggcaaggg gctatgaagt tgcaaaccaa
    15541 ataaatgctc gcgattctca accaccccag gtgcatagcg gtgccgccag tgcggataga
    15601 tttcatatac attcgaagcc tgccaatctt gaaagtcgta atccagcgca ttgaggcctg
    15661 tttcttcctt cacctcacga atcgcggcct gaaaaggggt ttcgtcacct tcaacgctgc
    15721 ccgtcaccga ctgccaaaaa cccgccttat cggcgcgctc catcaccagc acttgaaaat
    15781 ctgctgtatg aataagcacc aaggctgaaa cgggaatttt aaattttttc atgatgttgg
    15841 tgaagaggga gtggattcaa accgctttat ttaattttaa tcacagtacc aacaaaatcg
    15901 cttcagctaa acatgattag cgagatttaa atttttttac tagcttgcgt taagttcttt
    15961 gggagttgaa aaatcacatt ttcaaccacg ccttgtaact cttgcacatt cgccgcaccc
    16021 aaactttgta gtttgctaat aacatctttc actaatattt caggggctga agccccggca
    16081 gatacgccaa ttttcttttt accggtgagc cattcagggt taaggtggct ggcattatct
    16141 accatgtagg cttcaatgcc ctgattttgt gctacttcac gcaggcggtt tgaatttgag
    16201 ctgttgggcg aacccacgat gatgactaaa tcacaatctt tagccattat ttttacagcg
    16261 tcttggcggt tttgcgtggc ataacaaatg tcatcacttt ttggggcgcg gatcgccgga
    16321 aaacgcgctt gcaaagcatc gatgaccttt gctgcatcgt ccatggagag cgtggtttgt
    16381 gtgacgtaag ccaatttttc aggattgatt acctgtaagg caagtacatc atcgggtgtt
    16441 tcaaccaaat acatgccctg ctctgcctgc cccatggtgc cctcaacttc agggtggcct
    16501 ttgtggccaa tcatgataat ttccaagccg gctttgcgca ttttttcaac ttcaatatgc
    16561 actttggtca ccagcggaca tgtagcatcg taggcggtta agccgcgtgc ttcggcctca
    16621 aggcgtaccg cttttgaaac gccatgggcg ctaaaaataa cggtgctgcc cgctgggatt
    16681 ttatcgagtt cattgacaaa aatggcgcct tttttacgca attcatccac cacaaacttg
    16741 ttatgtacaa cttcattgcg cacataaatg ggtgcgccaa atttttctag tgcctgctct
    16801 acaataataa ttgcgcggtc aaccccggcg caaaagccgc gcgggttggc gagtaaaata
    16861 tctgcttggg tatttgccat atgctctttt tgttcatcaa atttaatgtc gggcaatgct
    16921 aatcattcaa ctttcgttgc caaaccgcgt tgcgcataaa aaataattcg ttatatatca
    16981 tatatatacc acctcattat ttatttattt gcgccttgtt tggcttagaa ttttgaatga
    17041 ttaacaacac ccttacggta taatgcgcat attaacctaa ataacacaag aaaacatgaa
    17101 caattcagcc gttaaaaaca ctgcaaccct actgattacg tgccctgata gcaaaggcct
    17161 tgtggcagcc attgcagaat ttttatatca gcataatgcc aatattttac atgctgacca
    17221 acatcaagat gccgaaaata acctgttttt aatgcgagtt gagtgggatt tgacagggtt
    17281 ttccatgccc atcgcagatt ttgaacagca tttcaccgcc attgccgtgc gttttaatat
    17341 ggaatggcaa ctgaaactct caaataaacc agtgcgcgta gcgattatgg tgtcgcaata
    17401 cgaccactgc ttggctgatt tgttgcaccg ccataaaaat ggcgagttag actgtgaaat
    17461 tccactcatt atcagcaacc atcgcgatac tgaaagtttg gcaaattttt atggcgtcga
    17521 ttttcactac attccagtaa ctaaagataa caaagccgct gcagaagccc agcagtttgc
    17581 attatttgag cagtatgcca ttgatttaat tgtacttgca cgatacatgc aaattttatc
    17641 gcctgatttt gtagcgcgct atcctaagca aattatcaac attcaccact catttttacc
    17701 tgcgtttatt ggtgcgcggc cgtatcatcg ggcctttgag cgcggcgtta agcttattgg
    17761 cgcaaccagc cattatgtga ctgaagtgtt ggatgaaggg ccaattattg aacaggatat
    17821 tgaccgcatt tcgcaccgcg accaagtgga agatttgatt caaaaaggcc gtgatttaga
    17881 gcggattgtg ctttcaaaag cggtgcgctg gcatattgaa aatcgtattt tattgtatgc
    17941 aaataaaacc gtgatttttg actaaatttg tatcaaacct tttgagatgc cctgcgagcc
    18001 ttgtaaataa aggctcgcag ggcagcaaaa tttcctcggt gcgttttaag gcttaaatta
    18061 gcaatttatt agcgtcaatt tttgagcaaa aaactaaact tttggcagcg tattctcttg
    18121 ggcaaataaa tctgccaccg tttcacgttt gcgaatcaaa tggcaagtct cgccatccac
    18181 catcacttcg gcggcacgtg ggcgcgtgtt gtaattgctt gccatgctca taccgtaagc
    18241 tccggcagac ataatcgcca gcaaatcacc ttcttcaagt gctagtgttc tgtcatggcc
    18301 gagaaaatcg ccactttcgc agactgggcc cacaatctcg tatgaaattg caggggtatt
    18361 acgcggtgtc accgcttgaa tttcatggta agcgtcataa agcgctgggc gcatcaggtc
    18421 gttcatggcc gcgtctacaa tcgcaaaatt cttgctctct gttggtttaa gatattcaac
    18481 ttgagtgagc aaaatccccg cgttacctac caatgcgcgg ccaggttcaa acaccagttt
    18541 aacctgacgc tgaccaagct tttcttgcat ggccgccgcg taggcgctaa attccggggg
    18601 agtttcatca ctgtagcaaa taccaatacc accgccggca tcaatgtgct gaatatgaat
    18661 accatgttgc tcgagcgcat caaccagcgt caacacgcgg tcaaatgcat ctaaaaaagg
    18721 cgataactcg gtaatttgcg agccgatatg gcaatccacg ccatgaatcg aaatattggg
    18781 catgtttgca gcttctaggt agatgttgag cgcatcctca tatgccacac caaatttatt
    18841 gttttttagc ccggtagaaa tatacggatg cgtttttgca tccacattcg ggttcacccg
    18901 taatgaaaca ggcgctactt tacccattgc gcccgccaca gtgttcagcc gaacaagctc
    18961 gctgcgagat tccacattaa agcaaaaaat acccgcgttc agcgccgcct gcatttcaac
    19021 agccgtttta cccacgcctg aaaatacaat ttttttcggg tcgccacccg ccgccaacac
    19081 ccgcgccaat tcgccaccag acacaatatc aaaccccgcg cctaaacttg caaacaagtt
    19141 aagaatcgcc aaattagggt tagatttcac cgcaaaacac accaaatgat tactgccctt
    19201 aaagccagcg ttaaagcttt caaacgcatg tatgagcgcg gtttttgaat acacataaca
    19261 aggggttgaa aagcgcttgg caatatctgt gagcgctaca ttttcagcga ataaagtatt
    19321 gtttttaaga gtaaaagtat tcacggcaaa ggcttaagaa atatgaaaat gggtcgtaat
    19381 aaatgagcgg ctattatttt tcggccggct gtggatattg ctgctcagga atatacaaag
    19441 gcccttttgt gccgcaacca ctagcaaccg cacataaagc ggccagtaac aataaataag
    19501 caatattttt gtgcatagca aacagccctg cctgtaaaaa gtagaagtgc attttaacat
    19561 tcaataagca aggtaaatga ttcttcacag acaaaattta actataaacg ctaataaaaa
    19621 tacaaaaaac aaccgcttat cgcgcaaaac aaaccgatta tcagctactg ctagacatag
    19681 acatccataa aagtgcatgc ttcaattaac caaaaaagga aataccctat gaaacttact
    19741 gttcaacaac tgaatacacc cattgcaaac ttgccgcgca aagctcagca gggctttact
    19801 ttaattgagc tgatgatcgt cgtagcgatt attgggattc tagcttcaat tgctatacca
    19861 gcttacagtg attatgtgaa gcgaggtaag gcggctgaag cgacttcaac tttggcaaat
    19921 ttaagagtac aaattgagca gtactatcaa gacaatcgca cttatgtggg atttgcctgt
    19981 aaccctacgc agaaatactt tacctactct tgtacaacac aaaccgcaac cacctatact
    20041 ttgaatgcgg caggaaactc ggctcaagac atgggttcat tcgagtttac tttgaatcag
    20101 gccaacgcaa gaacctccaa atttgatggt acaacaggtg caacatgctg gctaactagc
    20161 aaaggcggat catgctagtg ttagcaaaaa acagaggtaa aactagccat cgtggtttta
    20221 ctttaattga gttgatgacg gtagttgctg tcattggtgt tttggcagca ttaggtttac
    20281 ctagctatcg catttggatt gaaaacacaa aaattcgcac cgcagcagaa tctatacaat
    20341 caggtttgca aaaagcacgt gtagaggcta ttaaacgcaa tgtgcgagtc cagtttgtgt
    20401 taggcgccaa ttcgacatgg actgtaagtt gcgttactgc tgcgcagtgt gcagatttag
    20461 cgggaggggt tgtagaaaca cgtggtgcga gtgatggttc atctgcaaat atcacggtta
    20521 ctgcaacgcc agcgggctca accacgatta ttttttcaaa ttttggcaca gtcagtccgg
    20581 catcagcaat tgcaccagtc gtaccagtac catttacgca actagaatta aataacgcag
    20641 cgctagactc agcagacagc agaaaactgc gcgttatgat tgggccaggc ggcgttggtc
    20701 gcacatgcga tccatattca ggtttatcaa cctctgatcc gcgtaaatgt taatggggaa
    20761 aatctgaata atgtttaaat ttaaatcaaa taattatcag gcatcaacaa tggtaaaaaa
    20821 atcccaacgc ggtattgcat tgttagagtc acttattggc atattgattt tttctatggg
    20881 catactcgcc attgctggcc tgcaaggatt tatgattaaa ggtactacag aagccaaata
    20941 ccgttcagac gcaagtgtga ttgcacaacg cagggtagca atgatgtggg cagatgcaaa
    21001 ggccaatcct gccgcactat ccgcagccta tagtgaggtg aatactgcta taccagattt
    21061 accgaatggc cgaagaacga ctacttttgt tgccgcaatt ccggatgctg tcacggtaac
    21121 agtgacttgg caaacaccag gtgagccgca gcataattat tcaacaaacg ccagaattac
    21181 tggggcaaga taatatgcgt ttcattcaac atcatcaatc agcaaaaagt tttaggcaag
    21241 ctggtttcag cttagttgag cttatggtcg gcttggttat agggctactg gccactttag
    21301 ccatcatgca aattttttca gcgttcgaag ggcaaaagcg tactacttct ggtaccgcag
    21361 atgcacaaac cagcggtagc gtagcgttgt acaccttgca aagagatgtg cagttggcag
    21421 gtttcggcct accactctat gatgaagcca atatgccatt gaagtgtaac gcaccatcat
    21481 atccagttgt cgatcacgat aataatgcag caacgccaaa tatcaatatg tcagcagtcg
    21541 atattatcaa tggcgccacg gcaacagata gcgacatcgt gattgttcgt tatggtgctt
    21601 cagcaagtgg gggtgtgcca gtgccagtgg ttggcgcacc taacgctgct
//
LOCUS       DAOQSI010000016        19459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_1942_length_19459_cov_6.809524, whole genome shotgun sequence.
ACCESSION   DAOQSI010000016 DAOQSI010000000
VERSION     DAOQSI010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 19459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19459
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1942_length_19459_cov_6.809524"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="PKL42_02220"
     CDS             <1..705
                     /locus_tag="PKL42_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNU65915.1"
                     /translation="SGDIVIALSNSGESDELLAIITPLKRLGASIIAITGNNASSLAL
                     AADIHLSASVSKEACPLGLAPTSSSTVTLALGDALALCVLDQRDFTAEDFARSHPGGS
                     LGRRLLIHVNDLMRKEAHIPSVSIDASLRQGLLEMSGKGLGLTAIVDADQQPIGIFTD
                     GDLRRAFEKNVNVATASIQEVMHRNPTTIHQNQLAIEAVEIMEQRKINALLVVDDAGK
                     LVGALNMHDLLLAKVV"
     gene            706..1272
                     /locus_tag="PKL42_02225"
     CDS             706..1272
                     /locus_tag="PKL42_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNU65916.1"
                     /translation="MQTLNTRISDEVIRRLKNIRLLVLDVDGVMTDGGLTIGDDGQEY
                     KTFHSHDGLGMKLLKASGVELAIITGRTSNVVKKRAESTGVAHFYQGAEDKLTAFEDL
                     VKISGLTPAQCAFMGDDVVDLPPMLQCGCAIAVPASPQLVLDHAHYITLKSGGHGAVR
                     EVCELIMQSQGTFDAQMAPFLKQARMAN"
     gene            1288..2286
                     /gene="lptC"
                     /locus_tag="PKL42_02230"
     CDS             1288..2286
                     /gene="lptC"
                     /locus_tag="PKL42_02230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNU65917.1"
                     /translation="MITKARSAIVFPITLLAILAILAAWINLIVQPPTPKPDGSSRHD
                     PDYIVNNFVTTQTDINGKLRYTLAASEMKHFPDNDATELKQPRYTQYTIDKPYTRVEG
                     QRGDVSSNGEEVKLYDQVKVTREAFAEKGEMTVETDYLEILPNQNLVRTDRPVIIRQA
                     PKTVIHATGMIYEKDKNTVTLLHKVKAHYERPPLKKSKVKKSTTNKQIQQPIAENTPK
                     TKLNKNNGVKSTSAQKTTTKPVSLKSNAKNTDSKKATNKQVTTTKAKPETKNNKKLAA
                     NNIKSNVKTSDVKKTKSAQSSATKTSTKSTSATKLSQPKSNQSKINAKEKRASEKL"
     gene            2283..2888
                     /gene="lptA"
                     /locus_tag="PKL42_02235"
     CDS             2283..2888
                     /gene="lptA"
                     /locus_tag="PKL42_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HNU65918.1"
                     /translation="MNNPVNITQPILGILALLNFITIIGFSTNALAEAADRDKPINLE
                     ADSVKVDDAKQISTYSGNVILTQGTLIIRADKLIVREDSAGFQHSTSTGNPTTFKQKR
                     EGKNEYMEGSAQRIEYDGRMDKVQLYTKAWVKRGEDIVHGEYISYDANAEYAEVIGGA
                     KSADGTTTSGRVRAIIQPKNKKAAGASTEQPKEAPKTQDTP"
     gene            2937..3659
                     /gene="lptB"
                     /locus_tag="PKL42_02240"
     CDS             2937..3659
                     /gene="lptB"
                     /locus_tag="PKL42_02240"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNU65919.1"
                     /translation="MSELIVENLRKSYKSRTVVQDISLHIKSGEVVGLLGPNGAGKTT
                     SFYMIVGLVSLDDGKITLDGNDLSHMPIHMRARMGLSYLPQEASIFRKLTVSENILAV
                     LQLQEISEEEMDARLESLLEELHITHIRDSAAVSLSGGERRRVEIARCLATDPDFILL
                     DEPFAGIDPIAVIDIQKIIHLLTTRNIGVLITDHNVRETLGICDRAYIVNQGHVFASG
                     IPSEIVENESVREVYLGKDFRL"
     gene            3656..5215
                     /locus_tag="PKL42_02245"
     CDS             3656..5215
                     /locus_tag="PKL42_02245"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNU65920.1"
                     /translation="MKQGLQLKFSQNLSLTPQLQQAIRLLQLSTLELNQEIDLLMQTN
                     PLLERGDDFEDEYGNATDGAEDEAAPPTSTQDLNSTGTTQELDEVGNDSDFTSTEFSE
                     GSAELAQSETTELEAGPTEFSAEFDDEFDEFSNGSLWDENTTPSDDDSDYKPQETLQM
                     SLREHLLSQLKLMPLSERDQTLCLLLIDSVSEDGYLEASLEEIAEEMPLELEIDELEL
                     QTALRLIQSLDPIGVGARDLSECLLLQLEALPKFTPHLALAKLLAKHYLPALGSRDFA
                     KLRKELGCDDATLKSVQQLITSLNPRPGSDFSVIGSEHYIQHEVIVKKVKGIWIATLN
                     DGVIPKLKINQMYANILKRNRDSNNQYLQSQMQEAKWMIKNIQQRFTTILRVSQAIVD
                     RQRNFLEYGEVAMKPLVLREIADELELHESTISRVTNNKYMLTPRGIFELKYFFGSSV
                     ATDSGGTASATAIRALIKKLVEQENPKKPFSDNQITELLAKQGIIVARRTIAKYRESL
                     GIEPASLRKTL"
     gene            5642..5983
                     /gene="raiA"
                     /locus_tag="PKL42_02250"
     CDS             5642..5983
                     /gene="raiA"
                     /locus_tag="PKL42_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNU65921.1"
                     /translation="MNLQLTGLHLEVTPALREYVTNKLSRISNHFDHVIDVKVTMSVE
                     KLIQKVEATLHVPGNDLHAACTDDNMYSAIDMLTDKLDRQVLKHKEKNADHHKSNGAV
                     KHQAVPNQDLN"
     gene            6142..7092
                     /gene="hprK"
                     /locus_tag="PKL42_02255"
     CDS             6142..7092
                     /gene="hprK"
                     /locus_tag="PKL42_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HNU65922.1"
                     /translation="MTQINVAELFKQTRRKLKLNWIAGLYGGANLLTSGTVTKPTLAL
                     IGHLNFVHPNRVQVLGCAEMDYLRSLKPTHTIAAIENLFSTDLAAVIVANNEAVPESL
                     IGAANQNNTPLFTTPLNSPELMDFLSHFLAQAVAESISMHGVFLEVQGFGAFIKGDPA
                     IGKSELALELISRGHRLIADDVVDFFRISPERIEGRCPALLQDFLEVRGLGVLNIRAM
                     FGDNSVKPSKPLDLIIQLEMADKLALDSLDRLNIKALHEEVLGVSISKVQIPIAAGRN
                     IAVLVEVAIRNHMLLLRGINATKQFTQRQQREMKKSNLSD"
     gene            7113..7979
                     /gene="rapZ"
                     /locus_tag="PKL42_02260"
     CDS             7113..7979
                     /gene="rapZ"
                     /locus_tag="PKL42_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNU65923.1"
                     /translation="MKQLIIVTGLSGSGKSIALRALEDSGYYCIDNLPATMLPQIAEH
                     LRANMQSGNLQVANHDRVAISIDSRSAAIETLPAHIKQLQSQQITTQVLFLESNVETL
                     VKRFSETRRKHPLSKDSTTLAESIASERALLADLGKLGHVIDTSYLSANTLRAWVKEV
                     VSIEHTGLTLLFTSFGFKHGIPLDADFVFDVRCLPNPHYDPNMRELTGRDASVQAFLS
                     AEPNVIEMIADIKAYIEKWLPCFVADNRSYLTVAIGCTGGQHRSVYFVEQLSQYFKST
                     QKVLTRHRELKL"
     gene            7988..8428
                     /locus_tag="PKL42_02265"
     CDS             7988..8428
                     /locus_tag="PKL42_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HNU65924.1"
                     /translation="MIGILIIAHGNLGESLMECAKHVIGNEPRQLAFLAVNTQDNPGE
                     LLPKAQQLIAALNTGDGVLVLSDMYGATPCNIVTKLLSPGIVEGVAGVNMPMIVRTMT
                     YRHESLMALVEKAVSGGREGVVHFSADSCDRFDTQAVTLNNKKS"
     gene            8444..8713
                     /locus_tag="PKL42_02270"
     CDS             8444..8713
                     /locus_tag="PKL42_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HNU65925.1"
                     /translation="MISTEVEIINKLGLHARASTKLTQTASKFASEIWISRNGRRVNA
                     KSIMGVMMLAAAKGSVVTLEANGTDEQTAIQAMTDLIANYFGEGE"
     gene            8721..10529
                     /gene="ptsP"
                     /locus_tag="PKL42_02275"
     CDS             8721..10529
                     /gene="ptsP"
                     /locus_tag="PKL42_02275"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNU65926.1"
                     /translation="MTGTTNVTAFSSPSFSLHGVGVSSGIAIGHAHLVSNALLEVVHY
                     QLPQHLIEDEIQRFSDAVATVKHDLEHIHASVRKNAPAEISAFISTHLMMLADKSLSE
                     TPKDIIRKELCNAEWAIKLQMDEVVEQFEQIEDDYLRERKQDVIQVVERVIKVLLGHP
                     HQLSSEQQASALHQERKLILVAHDISPADAIQFKQHEFAAFITDVGGTTSHTAILARS
                     LNIPSIVALQKARDLIHDGELIIVDGKQGVVIVNPDKSILTEYKLRQEQWELEQQKLQ
                     RIKSTKAITLDGANIELFANIEVPEDVVAAKASGATGIGLYRTEFLFMNRREMPSEEE
                     QFIAYKTVAEAMKGQPVIIRTLDLGADKQLSGESETECANPALGLRAIRYCLSEPQIF
                     HTQLRALLRASAFGQIKILFPMLSALSELRQSKLLLTRAKDSLRKENIPFNENISLGG
                     MIEIPAAAINAEAFANELDFLSIGTNDLIQYTLAIDRTDDSVAYLYNPLHPAVLKLIS
                     ITIKAANKLGKPISVCGEMAGEAKLTKLLLGMGLRQFSMHPSHILSVKQQILHSQVNQ
                     LTSHAKKILALSDIEKIEPLLTKFYTPIEMRTKARH"
     gene            10570..10917
                     /locus_tag="PKL42_02280"
     CDS             10570..10917
                     /locus_tag="PKL42_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HNU65927.1"
                     /translation="MTTELEVIVPSNDEKNIATVTHLGGTVFSFLPALIVWILKKDDS
                     AYLADQAKEALNFQITVLLAYIAAGVLSWILIGLLFFPIIWIVNIVFCIIAAIATSKG
                     ETYRYPLCLRLIN"
     gene            10940..12967
                     /locus_tag="PKL42_02285"
     CDS             10940..12967
                     /locus_tag="PKL42_02285"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNU65928.1"
                     /translation="MSNPLLHFVGLPKFEEIKAEHVSPAIDFLLSEARTTVNSLATSE
                     EAPSWQNYVLPLEDMEEKLSRAWSQVGHMNGVVNSPELREAYNENLTKLTDFYADLGQ
                     DERLYAKFRALKNSAEFAKLTSTQQKIIENELRDFRLGGAELPAEQKVRFKAIQEELS
                     KLSSKFEENVLDNTNDYALYVEDTSKLAGIPEDVLQGAKTAAETDKKVGYKFTLHFPS
                     YLPVLQYAQNRELRETLYRAYATKASEFGKKEWDNTPIIAQILKLKQEVAHLLGFANY
                     AELSIETKMAESANHVTQFLQDLAKKAKPYAIKDMAELTEYAAKLGIHDMQAWDVAYL
                     SEKLREEKYAFSDLEVKQYFPENKVLAGLFKVVETIFGVRVTKSHASVWHETASFYDI
                     SSHDGKLIGQFYLDLYARNNKRGGAWMDEAITRRKVASKITTPVAFLTCNFSAPVGDK
                     PALFTHDEVLTMFHEFGHGIHHMLTEVDDYSVSGIKGVEWDAVELPSQLMENFCWEWR
                     VLQNMTAHAETGKPLPRELFDKMVAAKNFQAGMQTMRQIEFALFDIRLHSTLDPNTNK
                     TALDLIEEVRDEVAVVRPPKWNRFPNSFTHIFSGGYAAGYYSYKWAEVLSADAYSLFE
                     ENDVLCAETGKRYWQEILAKGGSRPAMESFVAFRGREPSIDALLRHNGMAN"
     gene            12987..13625
                     /gene="tmpT"
                     /locus_tag="PKL42_02290"
     CDS             12987..13625
                     /gene="tmpT"
                     /locus_tag="PKL42_02290"
                     /EC_number="2.1.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008119 - thiopurine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HNU65929.1"
                     /translation="MHHNFWHQRWQENQIGFHLPEANPFLVKHLTALHLKQGARIFLP
                     LCGKTLDIAWLLSQGYKVAGAELSEIAIEALFAQLQLKPAIQQIGEITLYSAPNIDIF
                     VGDIFNVSAAMLGYVDAIYDRAALVALPDQIRQQYSGHLMTLTNHAPQLLICFEYDQA
                     AHAGPPFSTCAREVLQHYQAAYDVNLLASETIPDGLKGQHPATEHVWLLKPR"
     gene            13672..14445
                     /gene="xth"
                     /locus_tag="PKL42_02295"
     CDS             13672..14445
                     /gene="xth"
                     /locus_tag="PKL42_02295"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNU65930.1"
                     /translation="MKLATWNVNSLNVRLPHVLDWLRDNPIDVLCLQETKQEDSKFPY
                     DNLKAAGYHAIHSGQKTYNGVAILSKHAMSDVEYGIANFEDEQKRVISATINGVRVVC
                     VYVVNGQAVDSDKYAYKMRWLKALNAWLADELKKYPNLVLLGDYNIAPEDRDCHDPAA
                     WVGQVLVSPPEREAFQQLIALGLHDSFRLFEQPEKSFSWWDYRMMGFRRNFGMRIDHI
                     LVSDALKAKCTAAYIDKNPRKLERPSDHTPVILALQASS"
     gene            complement(14590..15948)
                     /locus_tag="PKL42_02300"
     CDS             complement(14590..15948)
                     /locus_tag="PKL42_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNU65931.1"
                     /translation="MASKNILVVDDEIGIRELLRDILQDEGYQVQLAENAASARAIRL
                     QERPDIVLLDIWMPDCDGITLLKEWANNSLLTMPVVMMSGHGTIDTAVEATRIGAFDF
                     LEKPIALQKLLKTVESALKHSEQLPKSEMNLSSLGKSPIISALKERLDKIAMSVNPSP
                     MLLIGPKGCGAELCARYLHQPGSPWLQLTEFNQLATAPIEVLEATRDGVVFISEVAEL
                     NKIEQKGLLLLITKAEKYSTRVVCGTSENLPKLQAESLFDHNLLQLLSAVSLRVPALD
                     EHKEDIPDLVVAIGNLQLELADVEYREFDVAALNALRNADWPGDLAQLDAVIRNLIQT
                     SLGEKITLEDVKRVLTQFEHPKALPEEQPSDATAVNLLATNNASVTQDKTEAKAAENQ
                     TWQPNFNQPLREARDEFERMYIQYHMKKVSNNMSKLAEIAGLERTHLYRKLKQLSIKI
                     KN"
     gene            complement(16030..18294)
                     /locus_tag="PKL42_02305"
     CDS             complement(16030..18294)
                     /locus_tag="PKL42_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNU65932.1"
                     /translation="MKYVAFISTVLGAFLLYLLSNASANTAASGAYYTLLVTLNIVLA
                     IFLVLVIGYQIWRISRQIRKRVVGSRFTLRLLTSFAMMALIPGLIVYLVSVNFLTRSI
                     ESWFNVKVEAALEGGLNLGRTALDIMLADLKEKGESMAISLAFQPDKARSILNDLREK
                     SGIEDVTLLTVQGRILEVSSSDSATFLPELPSVAQLRAAQKHTVSSVEPIGNKGLYLR
                     VLTPVSGQTLVGDKHILQLLQPVPKQLATTAESVQDVYQDYQQLSFSRASLREVFALT
                     LTLVMMLAMLGAVSIAFVLSRRLSTPLTVLAEGTKAIASGDYSTVLPAHGKDELGVLV
                     QSFNSMTQQLGDATKAADSNRARVEAARRYLETILAHLSSGVLALNQHGELRTFNEAA
                     MHILDVPLAPYVGFTPDRIIEKQPRLAIFFQEIAADLQQDKISADMAINEPELSKNTT
                     NHQPQKDEKQTQIELLNAHGKQILTLRGTPLPDGGYVVVFDDATTMVQAQRDAAWGEV
                     ARRLAHEIKNPLTPIQLSAERMAHKLQNKLEGADAQMLKRSTETIVNQVDAMKKMVNE
                     FSEYARAPAPKMQPLNLNQLIEEVVSFYELPKIKVLLSLEKQACDIQGDSTMLRQVIH
                     NLLQNAQDAMTNHPHTMPPTAQIKVTTHIEGNMLQLRVEDNGAGFPAEMLLHAFEPYM
                     TTKPHGTGLGLAIVKKIVEEHKGSIKIENIKPKVAHDTHDDKVEPISNNSQTGAVVTI
                     SIPLLKHHVGSRLQ"
     gene            complement(18361..18912)
                     /locus_tag="PKL42_02310"
     CDS             complement(18361..18912)
                     /locus_tag="PKL42_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HNU65933.1"
                     /translation="MAQSAYANSTSLTIRTAELVAVDDGYVLNADVDMKFSAKVEEAI
                     SKGFVLNFLVEFQLAKPRKYWFDDEVVTVVHALALSYHALSRQYLVTRGDQQKAFVRL
                     DEALDDLSDISGLKVLKKSEVDLDEAYKAAILMRLDTKKLPQALQGVANDGDWGMRSQ
                     RFEWAPNFTHHPAKTEIKLELAK"
     gene            complement(18944..>19459)
                     /locus_tag="PKL42_02315"
     CDS             complement(18944..>19459)
                     /locus_tag="PKL42_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HNU65934.1"
                     /translation="VLELVDVKMTALDSDEARLERVQSNLNRLNLQADLRVGDAASTD
                     WWDGIAFDRILADVPCSASGIVRRHVDIKWLRREADIASFSAQQAKILANLWQMLAKG
                     GKLLYVTCSVFNEENQQQVNQFLQQNANAIQLPFVLPAQYPQAGMVQMNGQLIPNNAH
                     DGFFYALLQKN"
BASE COUNT         5589 a         4353 c         4327 g         5190 t
ORIGIN      
        1 tcaggtgaca ttgtcatcgc actttcaaat tctggtgaaa gcgatgaact gttggccatc
       61 attactccgc taaaacggtt gggggcgagc attattgcaa taaccggaaa taacgcctca
      121 tctttagcac tggcggctga cattcatcta agcgcgagcg tgagcaaaga agcctgccca
      181 ttaggacttg cgccaacttc tagcagtact gtcaccttag cactgggcga cgccttagcg
      241 ctatgcgtgc tggatcagcg tgactttacc gctgaagact ttgcgcgctc acacccgggc
      301 ggcagccttg gacggcgctt gctcatccat gtgaacgact taatgcgcaa agaggcacac
      361 attccttctg tttcaataga tgcctcgctc aggcaagggc tacttgaaat gtcaggcaaa
      421 ggtttgggac tcacggcgat tgtggatgcg gatcagcaac ccatcggcat tttcacagat
      481 ggcgacttaa gacgcgcttt tgaaaaaaat gtgaatgtcg ctaccgctag catccaagag
      541 gtcatgcacc gcaaccccac caccatacat caaaatcaac tggccattga agcagttgaa
      601 atcatggaac aacgtaaaat caacgcgctg ttagtcgtcg atgatgcggg caaattggtg
      661 ggcgctttaa atatgcacga tctattattg gcaaaggtag tgtaaatgca aacactgaat
      721 acgcgcatta gtgatgaggt catacgccgt ttaaaaaaca tcaggctgtt ggtattggat
      781 gtagatggcg tgatgacgga tggtggcctg acgattggtg atgatgggca ggaatataaa
      841 acttttcatt ctcatgatgg cttgggcatg aagctgctta aagcctcagg tgtagagctg
      901 gctattatta ccgggcgcac ttcgaatgtg gttaagaaac gggctgaaag cactggggtt
      961 gcccattttt accaaggtgc ggaagataag ctcacggctt tcgaagactt agttaaaatt
     1021 tctggcttaa cacccgcgca atgtgctttt atgggcgatg atgtggttga tttgccaccc
     1081 atgttgcaat gtggctgtgc tatcgcggtg cccgcttcac cacagcttgt gttagatcat
     1141 gcacattaca tcacactaaa atctggtggg catggtgcgg tacgtgaagt atgcgaactt
     1201 ataatgcagt cacaaggcac atttgatgca caaatggcgc cctttctaaa acaagcccgc
     1261 atggcaaact aactgttaag tgtgtgaatg attaccaaag cacgttcagc gattgttttc
     1321 ccaattactt tattagccat tttggcaata ttagcggctt ggattaacct gattgtgcag
     1381 ccacctacac caaaaccaga tggctcaagc cgacatgacc cagattacat tgttaataat
     1441 tttgtcacca cgcaaactga tattaacgga aagttgcgtt ataccttggc tgcgtctgaa
     1501 atgaagcact tccctgataa tgatgcaaca gaattgaaac aaccgcgcta cacacaatat
     1561 acaattgaca agccctatac ccgtgtagaa ggtcaacggg gggacgtttc cagcaatggc
     1621 gaagaagtaa agctatatga ccaagtcaaa gtgacgcgcg aagcgtttgc tgaaaaaggt
     1681 gaaatgacag tagaaacaga ctatctggaa attctaccaa atcaaaattt agtgcgcact
     1741 gacagacctg tcattattcg tcaggcgcct aaaaccgtta ttcacgcaac ggggatgatt
     1801 tacgaaaaag ataaaaatac ggtcacgttg ttgcataaag taaaggcaca ttatgaaaga
     1861 ccgccattga aaaaatcaaa agttaaaaaa agtacgacaa ataagcaaat tcagcagcca
     1921 attgcagaaa atacgcctaa aacaaaattg aataaaaata atggcgtgaa atcgaccagt
     1981 gcacaaaaaa cgacaactaa acctgtgtca ttaaaatcta atgctaaaaa caccgacagc
     2041 aagaaggcaa caaacaaaca agtaaccaca actaaagcga aaccggaaac aaagaataac
     2101 aaaaagctcg cggctaacaa cattaaaagc aatgttaaaa catcggatgt gaaaaaaaca
     2161 aaatccgcgc agtcgagtgc gactaaaaca tcgactaaaa gtaccagtgc aactaaacta
     2221 tctcaaccaa aatcaaacca atcgaaaatc aacgctaaag aaaaacgtgc ttcggagaaa
     2281 ttatgaacaa ccctgtcaac attacacaac ccattttagg catcctagcg ctgttgaatt
     2341 tcatcaccat aattggtttt tctacaaatg ctttagctga agccgccgac cgcgataaac
     2401 cgattaactt agaagccgac agcgttaagg tagatgacgc aaagcagatt agcacctatt
     2461 ctggcaatgt gattttaacg caaggcactt taattattcg tgcggataaa ctcatcgtgc
     2521 gtgaagacag tgcaggtttt cagcacagca cttccactgg caaccccacg acctttaagc
     2581 aaaagcgaga aggtaaaaat gaatacatgg aaggcagcgc acaacgtatt gaatatgatg
     2641 gccgcatgga taaagtgcag ctctacacca aagcgtgggt caagcgcggt gaagatattg
     2701 tgcatggtga gtatattagc tacgacgcca atgctgaata tgctgaagtg attggtggcg
     2761 caaaatctgc ggatggcacc accaccagcg gccgtgtacg tgccattatt cagcccaaaa
     2821 ataaaaaggc cgcaggtgct agcactgagc aacctaaaga agcgccaaaa acacaagata
     2881 ctccttaata tttaatacca ttattacatt tgatgcagaa ataagaataa acgcatatga
     2941 gcgaactcat tgttgaaaat ctacgtaaat catacaaatc acgcacagtg gtgcaggata
     3001 tttctttgca cataaaaagc ggtgaagttg tcggcttatt agggcccaat ggtgccggca
     3061 aaaccaccag tttttatatg atagtcggct tggtgagttt agatgatggg aaaatcaccc
     3121 tcgatggcaa tgacttgagc cacatgccca ttcacatgcg cgccagaatg ggcttgtctt
     3181 atttgccgca ggaagcttct atttttcgta aactcaccgt atcagaaaat attctcgccg
     3241 ttttacaatt gcaagaaata tcagaagaag aaatggatgc gagattagaa tcgctgctcg
     3301 aagaactgca tatcacccat attcgagaca gtgccgcagt cagtttatca ggtggcgagc
     3361 gtcgccgtgt tgaaatagcc cgatgtttgg caacggaccc tgacttcatt ttattggacg
     3421 agccatttgc cggcattgac cccattgcgg tcattgatat acaaaaaatc atccacttac
     3481 ttaccacgcg caatattggc gtgctgatta ccgaccacaa cgttcgcgaa actttaggaa
     3541 tttgtgaccg cgcctacatt gtcaaccaag gccacgtatt tgcttcaggc attccgagtg
     3601 aaattgttga aaatgaaagt gtgcgtgaag tgtatttagg aaaagatttt cgtctatgaa
     3661 acaaggttta cagcttaaat tttcacaaaa tctctcgctt acgccgcaac tgcaacaggc
     3721 cattcggctt ttgcagctct ctacgttaga acttaatcaa gaaattgatt tgttgatgca
     3781 aaccaacccg ctattagagc gcggagatga ttttgaagat gagtacggca atgcgacgga
     3841 tggggcagaa gatgaagctg cgcccccgac aagcacacaa gatttaaata gcactggtac
     3901 aacgcaagaa ctggatgaag ttggcaacga ttcagatttc acaagcaccg agttttctga
     3961 aggtagtgcc gaattagcgc aatctgaaac tacagaactt gaagcgggcc ccacagagtt
     4021 ttcggctgaa tttgatgacg aatttgacga attttctaac ggcagcctgt gggatgaaaa
     4081 caccacgcca tccgatgacg atagcgacta caaaccgcaa gaaaccttgc aaatgagcct
     4141 gcgcgagcat ttgctctcgc aattaaaact catgccgtta tcagagcgcg accaaacgct
     4201 ttgcctatta ttgattgaca gcgttagtga ggatggctat ttagaagcct ctttggaaga
     4261 aatcgctgag gaaatgccgc tcgagcttga aattgacgaa ctggaattac aaacggcact
     4321 caggttgatt caaagccttg acccgattgg tgtcggggcg cgagacctaa gtgagtgcct
     4381 actattgcag ctagaagcac tgccaaaatt cacaccacat ttagcgctcg ccaaactact
     4441 tgccaaacat tatctgcccg cacttggctc acgcgatttt gccaaactgc gtaaagaact
     4501 cggctgtgat gatgccacgc ttaaaagtgt acagcaattg atcaccagct taaatccgcg
     4561 ccctggcagt gattttagcg tgattggctc agagcattac atacagcatg aagtgattgt
     4621 taaaaaagtg aaaggcatct ggattgccac cttgaacgac ggcgtgattc caaaattaaa
     4681 aatcaatcaa atgtacgcca acattttaaa acgcaatcgc gatagcaata atcaatacct
     4741 gcaaagccaa atgcaagaag caaaatggat gattaaaaac attcagcagc gtttcactac
     4801 tattttgcgc gtttcacaag ccattgtaga ccgccagcgc aactttttag agtacggcga
     4861 agttgccatg aaaccgctgg tgttacgcga aattgcggat gagcttgaac tgcatgaatc
     4921 caccatttcc cgcgtgacca acaataaata catgctcaca ccgcgtggta tttttgaact
     4981 gaaatatttc tttggcagct ccgttgccac agattcgggc ggaacagcat cagccaccgc
     5041 cattcgcgca ctgattaaaa agttagtgga gcaagaaaat ccaaaaaaac cattttcgga
     5101 caatcaaatt actgaattac tcgccaaaca gggcattatt gtggcgcgcc gcaccattgc
     5161 aaaatatcgc gaatcgcttg gtattgaacc tgccagttta agaaaaaccc tctaaacttt
     5221 taccctaaat tggtacgaaa gttttgcgcg tgctgaatgg taaaatgatt cgtttttttg
     5281 cacattttaa gcacaacctt tttcgatgct ctgcgagcct tataaataaa ggctcgcaga
     5341 gcataaaatt tttctcgtaa gattcttgat tggtattaga agttattaat cgccttaaat
     5401 catcaagttt tatacgtatt ttttcgcctg cgcttttatt taaggcttat tttttaccac
     5461 tatatacaat ttaaaatgat tgtaaatttt attaaaaaaa tttatgaatc tgcctagcac
     5521 agacacgaat taaagtagta aactgaaatt gttctgagag ttttgcgttg aattaaaatt
     5581 ttcaaacttt gtagattttt ttgaattcaa gcttttagaa tattttaata aggagcaagt
     5641 catgaattta caattaactg gtctgcattt agaagtaacc ccagcattac gtgaatatgt
     5701 taccaacaaa ctttcacgta ttagcaacca ttttgaccat gtaatcgatg tcaaagtgac
     5761 catgagcgta gaaaaactta tccaaaaggt agaagctacg ctacacgttc ccggtaatga
     5821 tttacacgcc gcttgtaccg atgacaatat gtacagcgca attgatatgt tgacggacaa
     5881 acttgaccgt caggtgctta aacataaaga aaaaaatgct gatcaccata aatcgaatgg
     5941 cgcagtgaag catcaagcag ttccgaatca agatttaaat tagtgttaat ttctctttga
     6001 gcattggatg cctctatcaa ttcaagttta tatgacaaac tgggtgcaat taaaaaatta
     6061 aggttcggtc tggcatatca tgcgtggttt ttatgtgtgc cgccgtatcg tgataaaact
     6121 tgaattgata gaggtatcca agtgactcaa atcaatgtag ctgaattatt caaacaaacc
     6181 cgccgtaaac tcaaattaaa ttggattgcc gggctatatg gcggggctaa tttactgacc
     6241 agcggcaccg ttacaaaacc aacgcttgcg ctcattggcc acctgaattt tgtgcatcct
     6301 aatcgggtgc aagtgctagg ctgtgccgaa atggattacc ttcggagttt aaagccaaca
     6361 cataccattg cagcaattga aaatttgttt tccacagacc ttgctgcagt gattgtggcc
     6421 aacaatgagg ctgtgccaga gtctttgatt ggcgcggcca accaaaataa cacgccgcta
     6481 tttacaacgc cgcttaacag ccctgaatta atggattttc tgagtcactt cttggcgcaa
     6541 gccgtggcag aatccatcag catgcacggc gtgtttttag aagtacaagg ctttggcgct
     6601 tttattaaag gtgaccccgc cattggtaag agtgagttag cgctagagct tatttctcgt
     6661 gggcatcggc tgattgcaga tgatgtggtc gatttttttc gtatttcacc agagcgtatc
     6721 gaaggccgct gcccggcact gctgcaagac tttttagagg tgcgtggttt gggcgtattg
     6781 aatattcgtg cgatgtttgg tgacaattca gtaaagccca gcaagccgct tgatctcatt
     6841 attcaacttg aaatggcaga taaactggcg cttgattcgc tagaccgcct caacatcaaa
     6901 gcgctccacg aagaagtact tggggtgagt atttctaaag tacaaattcc gattgcggca
     6961 ggccgcaata ttgcagtatt ggttgaagtc gcaattcgca atcacatgtt attattgcgt
     7021 ggtattaacg cgaccaaaca attcacccaa cgccaacagc gcgaaatgaa aaaatctaac
     7081 ctgtcagatt aaatatcaag tacccgactt caatgaaaca acttatcatc gtcacaggac
     7141 tctccggctc aggcaaaagt attgccctaa gggcgcttga agacagcggc tattactgca
     7201 ttgataactt gcccgccacc atgttgccgc aaattgccga gcatttacgt gcaaatatgc
     7261 aatcgggaaa tttacaagtg gccaatcatg accgcgtggc catcagcatt gatagccgca
     7321 gtgcggcgat tgaaacgctc cctgcacaca ttaaacaact ccaatcacaa caaatcacga
     7381 cgcaagtgct ttttttagaa tctaacgttg aaaccttagt caaacgtttc agcgaaacta
     7441 ggcgtaagca ccctttaagt aaagacagta ccacgcttgc tgaaagcatc gcctctgagc
     7501 gcgcactact ggccgattta ggaaaactcg gacatgtgat tgatacgagc tatctgagcg
     7561 ccaataccct tagggcttgg gtcaaagaag tcgtctcgat tgaacatact ggtttaacgt
     7621 tactgtttac ttcgtttggc tttaaacatg gcattccact ggatgctgat tttgtatttg
     7681 atgtgcgttg tttacctaat ccgcattacg accccaacat gcgggaactc accggacgtg
     7741 atgcgtcggt acaggcattt ttaagcgcag agcctaatgt gattgagatg attgctgaca
     7801 tcaaagctta tattgaaaaa tggctgccat gctttgttgc agataaccgc agctatctaa
     7861 ccgtggccat cggctgcacg ggcgggcagc atcgctcagt ttattttgtt gaacaattga
     7921 gtcaatattt caaaagcacg caaaaagtac tgacgcgaca tcgtgaacta aaactgtaga
     7981 gattactatg attggtattt taattatcgc acatggcaac ttaggtgaaa gcctgatgga
     8041 atgcgctaaa cacgtcatcg gaaacgaacc gagacaattg gcttttctag cagtgaacac
     8101 ccaagataac cctggcgaat tactgcccaa agcgcagcaa ttgatcgccg cactgaacac
     8161 tggagacggt gttttagtgc tttcggatat gtatggtgcc accccttgta atattgtgac
     8221 taaactactc tcacctggca tcgttgaagg tgttgctggc gtgaatatgc ccatgattgt
     8281 gcgtacaatg acttaccgac acgagtcttt aatggccttg gtagaaaaag ccgtcagcgg
     8341 aggacgagag ggtgtagtgc atttcagcgc ggacagctgt gatcgattcg acactcaggc
     8401 agtcacgctt aataataaaa agtcataatg aatggattga ataatgatta gcacagaagt
     8461 tgaaatcatc aataaattag ggttacatgc acgcgcgtct accaaactca cccagacggc
     8521 aagcaagttt gccagtgaaa tctggatttc acgcaatggc cgccgtgtga atgctaaaag
     8581 catcatgggc gtgatgatgc tggccgcggc aaaaggcagc gttgttacct tggaagccaa
     8641 tggcacggat gagcagacgg ccatacaagc aatgactgac ttaattgcaa attactttgg
     8701 cgaaggcgag taatccaatc atgacaggca caaccaatgt gactgctttt agtagcccga
     8761 gcttttcact gcacggtgta ggcgtgtcaa gcggtattgc cattggtcac gcgcatttag
     8821 tttctaatgc gctgttagag gtggtgcatt accaattacc gcaacactta attgaagatg
     8881 aaattcaacg tttttctgat gccgttgcaa ccgtcaagca cgatttagag catatccatg
     8941 cgagcgtgcg aaagaatgcc cccgctgaaa tcagcgcgtt catcagcaca catctcatga
     9001 tgctggccga taaatcgttg agtgaaaccc ctaaagacat catcagaaaa gaactatgca
     9061 acgctgaatg ggcgatcaaa ctgcaaatgg atgaagttgt tgagcagttt gagcaaattg
     9121 aagatgatta tctgcgtgaa cgcaaacaag atgtcattca ggtggtcgag cgcgtcatta
     9181 aagtgctgct aggccacccg catcaattaa gctctgagca gcaggcaagc gctctgcacc
     9241 aagaacgcaa gctcatttta gtcgcccacg atatttcgcc agccgatgca attcagttta
     9301 agcagcatga gtttgcggct tttattaccg atgtgggtgg tacaacttcg cataccgcca
     9361 ttttggcgcg cagtttaaat attccgtcaa ttgtcgctct gcaaaaagcc cgtgatttaa
     9421 ttcatgatgg tgaactcatc atcgtggatg gcaagcaagg cgtggtgatt gttaaccccg
     9481 ataaaagcat actgaccgag tacaagctgc gccaagaaca gtgggaactt gagcaacaaa
     9541 agttacaacg cattaaatca accaaagcca ttacgctaga cggcgcaaac attgagttat
     9601 ttgccaacat tgaagtgcca gaagatgttg tggcggctaa ggcatcaggg gcgacgggaa
     9661 ttggtctgta tcggactgaa tttttgttta tgaaccgccg cgaaatgcct agtgaagaag
     9721 aacagttcat cgcctataaa accgtggcag aggccatgaa gggtcagcct gtgattatcc
     9781 gcacgctgga tttaggggca gataaacaat taagtggcga gagcgaaaca gaatgcgcca
     9841 acccagcatt gggcctgcga gcgattcgct attgcttgag tgagccacag attttccaca
     9901 cacaactacg tgcattattg cgtgcttcag cttttggcca gattaaaatt ctcttcccga
     9961 tgctttcagc attatcagaa ttacggcaaa gcaaactatt gctcacgcgc gctaaagata
    10021 gtttgcgtaa agaaaatatc ccgtttaatg agaacatttc gttaggtggc atgatagaaa
    10081 ttccagccgc tgccatcaat gcggaagcat ttgcgaatga gttggatttc ttatccattg
    10141 gcaccaatga tttaattcaa tataccttag ctattgaccg tacggacgac agcgtcgcgt
    10201 acctttataa tcccctgcat ccggctgtat taaagctcat ttcaatcacc attaaagccg
    10261 ctaataaact aggtaaacca atttcagtgt gtggtgaaat ggctggcgaa gccaagctca
    10321 caaaactgtt gcttggtatg gggttgcgtc aattttctat gcacccttca catattttga
    10381 gtgtcaaaca gcaaattcta catagccaag ttaatcaact gacgagccat gccaaaaaaa
    10441 tattagccct cagtgatatt gaaaaaattg aaccgctgtt aaccaaattt tatacgccga
    10501 ttgaaatgcg cactaaagcc agacattaaa catttatttt ccaacgcttc aaatattgaa
    10561 aggattatca tgaccactga attagaagtc atcgttccaa gcaacgacga aaaaaatatc
    10621 gctaccgtta cgcatttagg tggcacggta ttttcatttt tacctgcatt aattgtttgg
    10681 atattaaaaa aagacgatag cgcctacctt gcggaccaag ctaaagaggc acttaacttt
    10741 caaatcacgg tcttacttgc ctatattgcc gctggcgtgc tttcttggat actcatcggg
    10801 cttttatttt ttccaattat ttggattgta aatattgttt tttgcatcat cgccgccatt
    10861 gctacaagta agggtgaaac ttatcgctac ccactgtgct taagactgat taactagttt
    10921 ttattgttga aggaattgtg tgtcaaaccc actattacac tttgttggcc tacctaaatt
    10981 tgaagaaatc aaagcagaac acgtctcgcc tgccatagac tttttactga gtgaagccag
    11041 aaccaccgtc aattcactcg cgacaagtga agaagcaccc tcttggcaaa attacgtatt
    11101 gccacttgaa gatatggaag aaaagttatc tcgcgcctgg tcgcaggtag gccacatgaa
    11161 tggcgtggtg aacagccctg aattacgtga agcgtataac gaaaatttaa caaaacttac
    11221 tgatttttat gcggatttag gccaagatga gcgtttatat gccaaatttc gagcgttaaa
    11281 aaatagcgct gaatttgcta agctcacttc aacacagcaa aaaattattg aaaatgaact
    11341 gcgtgacttt cgtttaggtg gtgccgagtt acctgcggag caaaaagtac gctttaaggc
    11401 aattcaagaa gagttatcaa aattatcatc aaagtttgaa gaaaacgtgt tggataacac
    11461 caatgactac gcgctttatg tagaagacac cagcaagtta gcaggcattc ctgaagacgt
    11521 attgcaaggc gccaaaacgg cagctgaaac ggataaaaaa gtgggctata aattcacttt
    11581 gcactttccc agctatttac ccgtgttgca atatgcgcaa aaccgcgagt tacgcgaaac
    11641 actttatcgt gcttacgcca ctaaagcctc agaatttggc aaaaaagaat gggataacac
    11701 gcctattatt gcgcaaattt taaaattaaa acaagaagtt gcgcatcttt taggctttgc
    11761 caattatgct gaactttcta ttgaaactaa aatggcggaa tcagccaatc acgtgaccca
    11821 atttttgcaa gatttagcca aaaaagccaa accttatgcc attaaagata tggctgaatt
    11881 gaccgaatac gccgctaaat taggcatcca cgatatgcaa gcgtgggatg tggcctattt
    11941 gagcgaaaaa ttacgtgaag aaaaatatgc gttttctgac ttagaagtga agcaatattt
    12001 ccccgaaaac aaagtgcttg ctggcttgtt taaagtagtt gaaactattt ttggcgtgcg
    12061 cgtcaccaaa tcgcacgcct cagtttggca tgaaacagcg agcttttatg acatcagcag
    12121 ccatgatggc aaattgattg ggcagtttta tttagatcta tatgcccgca acaacaaacg
    12181 cggtggcgct tggatggatg aagccattac ccgtcgtaaa gtggcaagca aaatcactac
    12241 acccgtcgca tttttaacct gtaacttctc cgccccagtt ggggataaac ctgcgttatt
    12301 tacgcatgat gaagtgctaa ccatgtttca tgaatttgga cacggcatac accacatgct
    12361 gactgaagtg gatgactaca gcgtttctgg cattaaaggt gtggaatggg atgccgtaga
    12421 actgcctagc cagttgatgg aaaatttttg ctgggagtgg cgtgtgctac aaaacatgac
    12481 ggcacatgca gaaactggca aacctctgcc acgcgagttg tttgacaaaa tggtcgcagc
    12541 caagaacttt caggccggca tgcaaaccat gcgccagatt gaatttgccc tatttgatat
    12601 tcgcctgcac agcacacttg accccaatac caacaaaact gcgctcgact taattgaaga
    12661 agtacgtgat gaagtagccg tggtgcgccc accaaaatgg aaccgctttc caaatagctt
    12721 tacgcatata ttctctggtg gctacgctgc tggctattac agctataaat gggcagaggt
    12781 gctttcagcc gacgcttaca gcctatttga agaaaatgac gtgctttgtg cggaaacggg
    12841 caaacgttat tggcaagaga ttttggctaa agggggaagc cgccctgcaa tggaatcgtt
    12901 tgtggctttt agaggcagag aaccgagcat tgatgcgctg ttacggcaca atgggatggc
    12961 gaattaaccc ctaaactgca gcctatatgc accacaactt ctggcaccaa aggtggcaag
    13021 aaaatcaaat tggctttcat ttgcctgagg ccaatccgtt tctggttaaa cacttaaccg
    13081 cgctccatct aaagcaaggc gcacgcattt ttttaccgct ttgtggtaaa actttggata
    13141 ttgcttggtt gctttcacaa ggttataaag tggctggagc agagttaagc gagattgcga
    13201 ttgaggcttt atttgcacaa ttgcaactta aacctgccat tcaacaaata ggtgaaatca
    13261 cgctttacag cgcacccaat atagatattt ttgtgggtga tatttttaac gtgagcgctg
    13321 ccatgctggg ctatgtggat gccatttatg accgtgcagc tttggtggct ttgcctgatc
    13381 agatacgcca gcaatatagc gggcatttaa tgacgctcac caaccatgcg ccgcaattgc
    13441 ttatttgctt tgagtatgac caagccgcgc atgcggggcc gcctttttca acctgcgcgc
    13501 gtgaagtatt gcagcattac caagccgctt acgatgtaaa tttgttagca agtgagacga
    13561 tacccgatgg cttaaaaggt caacatccag ccaccgagca tgtgtggttg ttaaagccgc
    13621 gctaactttt tagcgtgcta aaatggctta aatttttcta ggaaattcac cgtgaaactc
    13681 gctacttgga atgtgaattc actcaacgtg cgcctgccac acgtgctgga ttggctgcgc
    13741 gacaacccga ttgatgtgct ttgcctgcaa gaaaccaagc aagaagacag taagtttccg
    13801 tacgataact tgaaagcggc gggttaccac gcgattcaca gcgggcaaaa aacttataac
    13861 ggcgtggcga ttttaagtaa gcatgccatg agcgatgttg aatatggcat tgccaatttt
    13921 gaggatgaac aaaagcgcgt gatctctgcc accattaacg gggtgcgcgt ggtgtgcgtt
    13981 tacgtagtga atggccaagc ggtggattct gataaatacg catataaaat gcgttggcta
    14041 aaggcgctca atgcttggct tgcggatgag ctaaaaaaat atccgaattt agtattgctg
    14101 ggcgattaca acattgcgcc tgaagaccgt gattgccatg acccagctgc ttgggtgggg
    14161 caagtgttgg tttcaccgcc tgaacgtgag gcttttcaac aactaattgc tttaggcttg
    14221 catgacagct ttaggctatt tgagcaaccc gaaaaaagct ttagttggtg ggattatcgt
    14281 atgatggggt ttagacgcaa tttcggaatg cggattgacc atattttagt aagcgatgcg
    14341 ctcaaggcaa aatgcactgc ggcttatatt gataaaaatc cgcgcaaatt agagcgccca
    14401 tcagaccata cgcctgtcat tttggcgttg caggcttcat cataaaaaag tcttttttct
    14461 cgcacaaaaa acttacgaga aaaaataaat gctctgcaag cctttgttta taaggcttgc
    14521 agagcatcga aaaaggttgg gcttaaaaat gaggttttta aaggcaattt agcacaatat
    14581 tttaccgtgc taatttttaa tttttatgct caattgcttg agtttgcggt acaagtgcgt
    14641 gcgctccaaa ccagcaattt ctgccagctt gctcatgttg ttgctcactt ttttcatgtg
    14701 atattgaata tacattcgct caaactcatc acgcgcttca cgtagcggct gattgaaatt
    14761 gggttgccat gtttggtttt cagcggcttt tgcttcagtc ttatcctgcg tgacagaggc
    14821 gttgttggtt gccaaaagat tgaccgcagt tgcatcagat ggctgttctt caggtagggc
    14881 tttgggatgc tcaaattgcg tcaacaccct tttaacgtct tccagcgtaa ttttttcacc
    14941 tagactagtt tgaatcaggt tgcgaatcac ggcatctaac tgcgccaaat cgccgggcca
    15001 atcagcattg cgtagcgcat tgagtgcagc gacatcaaac tctcggtatt caacgtcggc
    15061 caattcaagt tgtaagttgc cgattgccac caccaaatca ggaatatctt ctttgtgttc
    15121 atccaaagca ggaacgcgta gcgacaccgc cgaaagtagc tgcaataaat tatggtcaaa
    15181 cagcgactct gcctgaagct tgggcaagtt ttcactagtg ccgcacacca cgcgtgtgct
    15241 gtatttttct gctttggtta tcagcaataa caggcctttt tgctcaattt tgttgagctc
    15301 ggccacttca ctgataaaca ctacaccgtc acgtgtggct tctaaaacct caatgggtgc
    15361 tgtggctagc tgattaaatt cagtaagttg tagccaagga cttccgggtt ggtgcaaata
    15421 gcgtgcgcat aactcagcac cgcagccttt cgggccaatg agcaacattg ggcttgggtt
    15481 cacactcatg gcaattttat ctaaccgctc ttttagcgca ctaataatcg ggcttttacc
    15541 caagctactc aaattcattt cagatttcgg taactgctca ctgtgcttga gcgcagattc
    15601 taccgttttg agtagttttt gcagggcaat aggcttttct aaaaaatcaa aagcgccaat
    15661 gcgcgtggct tcaaccgcag tatcaatggt gccgtgcccg gacatcatga ctactggcat
    15721 ggtcaacaaa ctgttgtttg cccattcttt taagagtgtg atgccatcgc aatcaggcat
    15781 ccaaatatcc agcaatacga tatctgggcg ctcttgtagg cgtatggcgc gggcagaggc
    15841 agcattttcg gctagttgca cttggtagcc ttcatcttgc aaaatgtcgc gcaataattc
    15901 gcgaatgcca atttcgtcat cgacgactaa tatgtttttt gatgccattg ttgtcttcta
    15961 tcgttgcttt ctcttattgt ctttagctgt tttcaaatca atacgttaat ttttaatccc
    16021 agtgcgttgt cattgtaaac ggctaccaac atgatgcttc aaaagcggga tgctgatggt
    16081 cacgacggca cctgtttggc tgttattaga aataggctct accttatcat catgcgtatc
    16141 atgcgccact tttggtttga tgttttcaat tttaatacta cctttgtgtt cttcaacgat
    16201 ttttttaaca atcgccagcc ccaaacccgt accatgcggt tttgtggtca tatagggttc
    16261 aaacgcatgc aaaagcatct ccgcaggaaa gcctgcgccg ttatcttcca cgcgtaattg
    16321 cagcatatta ccttcaatat gggttgtcac tttaatttgc gcagtgggcg gcattgtatg
    16381 tggatggttg gtcatcgcat cttgcgcatt ctgcaataaa ttatgaatca cttggcgaag
    16441 catggtgcta tccccttgaa tatcacaggc ttgcttttca agtgatagca gtactttaat
    16501 ttttggtaac tcataaaaag aaaccacttc ttctatcaac tgattcaaat ttaaaggctg
    16561 catttttggt gcaggcgcac gcgcatactc actgaattca ttcaccattt ttttcattgc
    16621 atccacttgg ttgacaatgg tttccgttga ccgctttagc atttgtgcgt ctgcaccttc
    16681 taatttattt tgcagcttat gcgccatgcg ctcggcagaa agctgtattg gcgtgagcgg
    16741 atttttaatt tcgtgcgcga gacggcgcgc gacttctccc cacgctgcat cgcgctgggc
    16801 ctgcaccatg gtcgtggcat catcaaacac gaccacatag ccgccatcgg gcaatggcgt
    16861 gccgcgcaag gttaaaattt gcttgccatg cgcattcaaa agctcaatct gcgtttgttt
    16921 ttcatctttt tgcggctgat ggttcgtggt gtttttgctg agttcaggtt cgttaatcgc
    16981 catgtcagcg cttattttat cttgttgaag gtcggccgca atttcttgaa agaaaatggc
    17041 tagccgcggc tgtttttcaa taattctatc gggcgtaaac cccacatagg gtgcaagcgg
    17101 cacgtctaaa atatgcatcg cagcttcatt aaatgtacgc aattcgccat gctgattaag
    17161 ggccagcacg ccagaagaca agtgtgcgag tatcgtctcg aggtaacgcc gtgccgcctc
    17221 tacgcgggca cggttactgt cagccgcttt tgttgcgtca cccagttgtt gcgtcatgct
    17281 gttgaatgat tgcactaata cgccaagctc atctttacca tgtgcgggta aaacggtact
    17341 gtagtctccg ctggcaatcg ctttggtgcc ctctgccagc acagtcagcg gtgtcgataa
    17401 tcggcggctg agtacaaagg caattgatac cgcacccaac atggcaagca tcatcactaa
    17461 agtcagcgta agcgcaaata cttctctgag tgacgcgcgg ctaaacgata gttgctgata
    17521 atcctgatac acatcttgca cactttccgc tgtggtggcg agctgctttg gcacgggctg
    17581 caataactgt aaaatatgct tatcgcccac cagtgtttgt ccgctcactg gagtaagtac
    17641 gcgcagataa aggcctttgt tgccgatggg ctctacactg ctaaccgtat gtttttgtgc
    17701 tgcccgcagt tgtgcaacac ttggtaattc aggtaaaaaa gtcgcagaat cactgctgga
    17761 aacttctaaa attctgcctt gtaccgtgag caaagtcaca tcctcaatac cgcttttctc
    17821 acgcagatca tttaaaatgg agcgggcttt atcaggctga aaagcaagac ttatcgccat
    17881 actttcccct ttttctttta agtctgccag cataatgtct agggccgtgc gacccaagtt
    17941 caagccgcct tcaagggctg cttctacttt tacattaaac caagattcaa ttgaacgcgt
    18001 taaaaaattc accgagacta aatacacaat caggcccggt atcaatgcca tcatggcaaa
    18061 actagtgagc aaacgcaggg taaagcggct accgacaaca cgcttacgaa tttgccggga
    18121 tattcgccaa atttggtagc caatcaccag cactaaaaaa atggccaaaa caatattgag
    18181 tgtcaccagc aatgtgtaat aagcgcccga ggcagccgta ttagcacttg cgtttgaaag
    18241 caagtacagc aaaaaagcgc ctaaaaccgt gctaatgaat gcgacatatt tcatgatgtg
    18301 gatttcatgg tctggatttc atggtgaaga ttttatttcg ccaatttcat ttcgtcaggt
    18361 ctatttcgct agctcgagct tgatttcagt tttggcagga tgatgggtaa aatttggtgc
    18421 ccactcaaag cgctgcgagc gcattcccca gtccccgtca ttggctaccc cctgcaaggc
    18481 ttgcggcagt tttttggtgt ccagccgcat caatatcgcg gctttataag cttcatcgag
    18541 gtcaacttca gattttttta acacttttag gccactgata tctgacaaat catccagcgc
    18601 ttcatctagc cgaacaaacg ctttttgctg atcaccacgc gtcactaaat actgtcgcga
    18661 aagcgcatga tagctcagtg caagcgcatg gactaccgtg acgacttcat cgtcaaacca
    18721 atattttctg ggcttagcga gttgaaactc caccaaaaaa ttcaatacaa agcctttgct
    18781 gatggcttct tcgacttttg cactaaattt catgtccaca tcggcattta aaacataccc
    18841 atcatccacg gcaacaagct cggccgttcg aatcgttaaa cttgtactat ttgcatatgc
    18901 actttgcgcc atcagcgcac acatgaaaaa aaacaaaagt tgcttaattt ttttgtagaa
    18961 gcgcatagaa aaaaccatcg tgagcattgt ttggaattaa ctgaccattc atctgcacca
    19021 tacctgcctg cggatactgc gctggtaaaa caaaaggcag ttggatggca ttggcgtttt
    19081 gctgtagaaa ttgattcact tgttgttgat tttcttcatt aaagacagaa caggttacat
    19141 aaagtaattt accacccttt gccaacatct gccataagtt ggccagaatt ttggcctgtt
    19201 gtgccgaaaa agaggcgatg tctgcctcac gccgcaacca tttaatatcc acatggcgcc
    19261 gcacgatgcc cgacgcactg caaggtacgt cggctaaaat tctgtcaaag gcaataccat
    19321 cccaccagtc tgttgatgcg gcatcaccca cacgtaaatc agcttgcaga tttagccgat
    19381 ttaaattgct ttgcacgcgt tctaggcgcg cttcgtcact atctagcgct gtcattttca
    19441 cgtctaccaa ttctagtac
//
LOCUS       DAOQSI010000017        19413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_1950_length_19413_cov_4.637463, whole genome shotgun sequence.
ACCESSION   DAOQSI010000017 DAOQSI010000000
VERSION     DAOQSI010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 19413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19413
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1950_length_19413_cov_4.637463"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1442)
                     /locus_tag="PKL42_02320"
     CDS             complement(<1..1442)
                     /locus_tag="PKL42_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="form I ribulose bisphosphate carboxylase large
                     subunit"
                     /protein_id="HNU65935.1"
                     /translation="MTTQSDARITDIKERYKGGVLKYKQMGYWRPDYEPKETDFICVF
                     RITPQEGVDPEEAAAAVAGESSTATWTVVWTDMLTAYEDYQGKAYRIDAVPGTGVGTD
                     KEAQYFAYIAYDLILFEEGSIANVTASLIGNVFSFKPLKAARLEDIRIPVAYVKTFKG
                     PPTGIIVERERLDKYGRPLLGATMKPKLGLSGKNYGRVVYEGLTGGLDFTKDDENINS
                     QPFMHWRDRFLFCMEAVNRAQANTGEIKGHYLNITAATMEDMYERAEFAKQLGSNIVM
                     IDLVVGWTAIQSISNWARKNDMILHMHRAGHGTYTRQKNHGVSFRVIAKWLRLAGVDH
                     LHAGTAVGKLEGDPMTVQGYYNVCRDAVTKQDLSRGIFFDQDWADLRKVMPVASGGIH
                     AGQMHQLLHLFGDDMVLQFGGGTIGHPDGIQAGAIANRVGIEVMVKARNEGKDILNEG
                     PDILREAGKWCKPLAQALETWKNVSFNYTST"
     gene            1561..2496
                     /locus_tag="PKL42_02325"
     CDS             1561..2496
                     /locus_tag="PKL42_02325"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNU65936.1"
                     /translation="MRNLTLRQVRIFLSAYKHMSFARAAEELCITAPAVSQQIKEMEQ
                     DMGVNMFIRENRKIELTSAGEYFLLYARKMLSTVNEADVMMERLRGTQMKTLNIGLVS
                     TAKYFLPHLIAEFKKDFPNLKIKMEARNRQQLVELLRDGEIDIAIMGQPPKEMDTRVE
                     AFAKHPHAFIANAQHALAGKLNITPDELNSYEVISRELGSGTRFIMEKYFTEHQISPI
                     VSMQISSIEAIKQAVIANLGITFTSLHTVGSEISNRQLVVLDVQETPIMRTWHVVALN
                     KHNNSQAAEAFRYFMLEKGGTLLNEMFKTIVTRNS"
     gene            complement(2701..3450)
                     /gene="rlmB"
                     /locus_tag="PKL42_02330"
     CDS             complement(2701..3450)
                     /gene="rlmB"
                     /locus_tag="PKL42_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNU65937.1"
                     /translation="MSDARILFGFHAVLSRLRQHAASVQEILIDRDRVDARMKDLLKM
                     AELSGVRTMEVERSRLDGMAGMNGRHQGVIARVVDTPIPYKDIHDILESDLTEPPFFL
                     ILDGVEDPHNLGACLRVADAMGVHAVIAPKDRAVGLNATVRKVACGAAETVPFIAVTN
                     LARTIRELKDAGVFVVGTTMDAPSNLLNTKLDGPIAIVLGAEGDGLRRLTIENCDALV
                     TIPMFGSVESLNVSVASGICLYEARRQRSLK"
     gene            complement(3816..6224)
                     /gene="rnr"
                     /locus_tag="PKL42_02335"
     CDS             complement(3816..6224)
                     /gene="rnr"
                     /locus_tag="PKL42_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HNU65938.1"
                     /translation="MSPSKKNSQKNNKRKLDPFFERESGKYEHPLPSREYILSVMTEQ
                     GVPLSLEQLYTLLDIGQDERDSFTKRLNAMEREGQIIQNRKGALCIADKLDLIAGVVQ
                     GHPDGFGFLIPDDKSKCNGEDLFLSAKEMSQVMHGDRAMVRMAGLDRRGRPEGKIVEV
                     LEHRTQRLVGRVLQTSGVTIVAAEDKRINLDILIPYHLDMGAKAGQVVMVELTSQPSL
                     HAQPMGKVVEILGNYADSGMEIEIALRKHNLPHEFSAEAVKLAESYPKLVQSADYKNR
                     IDCRELPLITIDGETARDFDDAVYAEPQGKGWRLVVAIADVSFYVRPGDALDKGAFER
                     GNSVYFPRRVIPMLPEALSNGLCSLNPDVERLCMICDMQVDGLGVVKQYKFYPSVMRS
                     KARMTYTQVYEMLQNPDGELAHEYAWLMPHLQHLNSVYQLMLTQREKRGAIEFETSET
                     IMVFNDQGKVESIIPSQRNEAHKLIEECMLAANVCAADFLLTHEHPALYRIHEGPTPE
                     KLELLRTFMGEFGFGVGGGDKPHAKDYGKLLARIRERPDAQLLQTVLLRSMQQAVYSP
                     DNVGHFGLAYEAYAHFTSPIRRYPDLLIHRAIKAVLKGERYKAGDWNALGTQCSMTER
                     RADDATRDVTNWLKCFYMQDKIGEVFEGTVAGVTSFGLFVALDGVYVEGLLHVTELGN
                     DYFHFDKARHEMAGERTGVRYRLGDRLTIKVARVDLETTKIDFTLINKTADINGDALQ
                     ASTGADYKGSKKPSGENVPKKTTLKLPEKAVKGSKTGAGKPAKKAVKKDRVRQSKNKG
                     KPKR"
     gene            6341..6425
                     /locus_tag="PKL42_02340"
     tRNA            6341..6425
                     /locus_tag="PKL42_02340"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6375..6377,aa:Leu,seq:cag)
     gene            6731..7498
                     /locus_tag="PKL42_02345"
     CDS             6731..7498
                     /locus_tag="PKL42_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNU65939.1"
                     /translation="MATTLENNHWDTLIDVIQRSYKIKKHVDFFRWQQDDLAKIFPHD
                     VLIAVWGDFNKGTLNYDVSSNIPGIRTQSLRESSGHIDHLMSDLYQRWLANSERWYRI
                     NNFSVDSDADGVPAFFNNQLSVINSLLVYGVRDIRGNNDCLYIFMDKQSEFPITSSVL
                     GMFMPHLDAALRRIESLESADAEEDDLSEYHVQGLSDREHEIIHWVRMGKTNFEISMI
                     LSISPNTVKNHLKRIFSKLDVSCRAQAVATYVPPKLN"
     gene            7561..8991
                     /locus_tag="PKL42_02350"
     CDS             7561..8991
                     /locus_tag="PKL42_02350"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HNU65940.1"
                     /translation="MAYRQSGSKTLLQRRSSLSNIVQQLLDGAAVIGIAWALIMRHMG
                     ALYSPYINLILVLLVVMALTYDRFAIYRSNGHAIDKAFNLLKAWTLTFFILVVLAFLT
                     KQSEIYSRNLITQLYIAGFIAQLIIHMLFRLVYKNIVQHAQQNEKVLIVGQSRLAYYL
                     QHRISDNPWVGEQVIGFVKLFDEDNKASGRKAAGRKNDPAVLGDVNDLPALIDLHDVS
                     TVYIVTPLKSSIELEKIYMDVIDKHVAIHWIPDIFALRLVNHSVKEISGIPVLTLSET
                     PLIGMRLLLKSIVDKVLSVLILLMIFPILIFVAIAVKIDSPGPVFFRQNRAGWSGKSF
                     RIWKFRSMYVHQEESGKLKQASKGDSRVTRVGAFIRKTSLDELPQIFNVLTGDMSLVG
                     PRPHALQHDKEYSQRIFDYFARHHIKPGITGLAQVRGLRGETVDIEQMVQRIESDIEY
                     INNWSIWLDISILFRTVFAFTGKGAY"
     gene            9114..10154
                     /locus_tag="PKL42_02355"
     CDS             9114..10154
                     /locus_tag="PKL42_02355"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNU65941.1"
                     /translation="MSSSLKSIKPILRDLLAEGLFISSISKPSRCANNKLTIVTFHRV
                     LPEELRQKYPYPGICVTPEELRWFCNFFNQFFTVGSLTYLHHRLMNGEHPEKPYLAIT
                     FDDGQLDNLQYGLPVLDEFNFKATFYIPARNVDEANILWHDRIGYCTLNAKNSGSEAM
                     KKVLILAEAYGVPSKVTDSFESHIIQNSKKIAPEKRTEFIENLENILKVTTPAWGRLM
                     TWSEIRQIHQQGHEIGSHSMTHALMPQCVDQDLKYEIVESRKRLESELNADITSFCYP
                     NGDYDERAIETVLQAGYRNAVTTKWGKNKPNQPQFQLDRCDINTFNNWNRKQELSVAR
                     FAFRLSGLQPGL"
     gene            10190..11416
                     /locus_tag="PKL42_02360"
     CDS             10190..11416
                     /locus_tag="PKL42_02360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNU65942.1"
                     /translation="MNLAYFCSEYPSRSHTFIRREIHELRRRGANIDVYALRKPKLEN
                     LISDTDHQEFKQTISILPIKPWQLVLVHGKMFFKNPLAYFKAFKVAFGHHLPGVKNTL
                     WSVFQFAEGIVLAHHLKQNPVQHLHIHFANSGANVGMIASEYLQLNWSMTLHGTSCFD
                     YPWGPLLGQKLEKCTFANCISYFGLSQAFRAVGPQHWDKLFVSRCGIELEAVNLAKSK
                     QSSQPKDATTIRLISVGRLHVEKGYPVMISAFAEALKTVPNMELVLLGDGPYRIALET
                     YVKEKGVSDKVIFCGAVPEQTVLDEMVHADIFALSSFMEGIPMVLMEAMILEVPVIAP
                     RLAGIPELVIDHENGLLFDPAHASQMAEHIVTLASDKEKRARFGATGKQKVLAEFTIE
                     KGVENLWQRFLKIEKN"
     gene            complement(11546..12562)
                     /gene="galE"
                     /locus_tag="PKL42_02365"
     CDS             complement(11546..12562)
                     /gene="galE"
                     /locus_tag="PKL42_02365"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNU65943.1"
                     /translation="MSVLVTGGAGYIGSHTCLELLNAGFDVVVIDNFSNSKFESLKRV
                     QQLTGRALKFKQGDVRDRNFLREVFSQHTITAVIHFAGHKAVGESIRLPLQYYDNNVS
                     GSIALAEVMSEFGVKSAVFSSSATVYDAPGDLQIPEDFPVKPINPYGQSKLMVEQIYR
                     DLSIADPTWKITILRYFNPVGAHESGQIGEDPNGIPNNLMPYISQVAVGRLEELSVFG
                     SDYPTVDGTGVRDYIHVVDLALGHLAALRTLEKQTGLVTVNLGTGKGCSVLQLVKAFE
                     TASGKPVKYRIVERRDGDIATCYANPQLAKSLFGWQAKFDIDAMCRDTWRWQFNNPLG
                     YNAS"
     gene            complement(12604..13425)
                     /locus_tag="PKL42_02370"
     CDS             complement(12604..13425)
                     /locus_tag="PKL42_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNU65944.1"
                     /translation="MYEAFYGLTEKPFSIQPDPDFLYFGQRHSLAYSMLEYGITNRSG
                     FTVVCGDIGCGKTTLVRHLLNNVNEDITVGIVYNTHQRVDDLLGWILLAFDQPYEGMS
                     EIALYEAFQKFLIESYTAGRRVVLIVDEAQNLSPSALESLRMLSNINADKDQLLQVVL
                     VGQPQLRELLRRPDLQQFFQRVAVDFFIPPLQVAEVSKYIEHRLKVAGCEKRLFTPRA
                     IELIAEASKGVPRSINILCDMVLVYGFSSGVEKIGVDLVEEVLRDRAEFGVLAYN"
     gene            complement(13543..14310)
                     /locus_tag="PKL42_02375"
     CDS             complement(13543..14310)
                     /locus_tag="PKL42_02375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNU65945.1"
                     /translation="MMNEIVEWKIRWYAMVDKIRVIADGVEEDALVKELSTSTYPRIL
                     GFVNSFAMNSMVTNEVFYNALHGADILLRDGSGMSMLMTRLAVNKGLNMNGTDFIPKL
                     LAAYRGKRVAFWGTEEPYLSQAVTKAVALYGVIPVSVHHGFESEQFYVDLAIETQPEF
                     IVLGMGMPKQEKIAAMIRDQNIATLVVCGGAIIDFMGNKVKRAPKWIRKIGFEWFYRL
                     VHEPKRLYRRYVLGNPAFIYRMLRMHRVVASRNKSHD"
     gene            complement(14320..15504)
                     /locus_tag="PKL42_02380"
     CDS             complement(14320..15504)
                     /locus_tag="PKL42_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNU65946.1"
                     /translation="MTLFFGVLLIVLGTVLLLPVVVFFTQVIFSSLLKLKETVVTVAR
                     PSFAVVVPAHNEQVQIRSTIASILPQLTSKDRLIVVADNCNDDTAKIARDAGAEVIER
                     FNLDARGKGYALDFGVRHCAETPPEVIVIVDADCQVEPNALGKLATTCIEFGRPVQAL
                     YLMKSPVGAGLKTKIAEFAWLVKNLVRPSGYAALGLPCQLMGTGMAFTWEGISKANLA
                     SGHIVEDLKLGIEFSQADKPPLFLKNALVTSVFPTNTEGLTGQRTRWEHGHLGVILSE
                     APSLFAEAIRKLNLNLLVLALDLIVPPLALLSLLIVGVFILSTVFYIVSGLLLPLVLS
                     GVGLLLLGIAVLVAWVRFGQQVISLASLCYAPIYALLKVPVYLNFFVKRQVNWVRAKR
                     DE"
     gene            complement(15501..15821)
                     /locus_tag="PKL42_02385"
     CDS             complement(15501..15821)
                     /locus_tag="PKL42_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65947.1"
                     /translation="MLRPMLNEMLMNPIRFFFALMLAFKMGWISERSLPYYLVFLAEA
                     CRILPWLNSHRSRHIHAHFGTNSTEVVMLARELGGLKYSFTVPGSQEFDRLQHLSLCE
                     RGAT"
     gene            complement(16231..16593)
                     /locus_tag="PKL42_02390"
     CDS             complement(16231..16593)
                     /locus_tag="PKL42_02390"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65948.1"
                     /translation="MSKLMVLRHRFWSVVTGADIPLTSKLGGGLLLPHPNGIVIHPDA
                     VIGPNCLIFQQVTIGMRAGSPPPTIGGHVDIGAGAKILGNVTIGNHACIGANAVVLKH
                     IPAGKSAVGIPAKVIDES"
     gene            complement(16782..17765)
                     /locus_tag="PKL42_02395"
     CDS             complement(16782..17765)
                     /locus_tag="PKL42_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNU65949.1"
                     /translation="MSISSRIGVVIIGRNEGERLVRCLKSLVQHTNQMVYVDSGSTDS
                     SISVATELGADIVALDMSQPFTAARARNEGYARLRKLFPHIEFVQFVDGDCEVNAQWL
                     ECATGFLDSQAQVAAVCGRRRERFPNKTVYNKLCDIEWDSPIGEAKACGGDVLMRANA
                     FESVHGFRAGLIAGEEPELCVRLRAAGWKVWRLEQEMTLHDAAMTTFGQWWKRTMRAG
                     HAFAEGAYLHGAPPERHWVQESKRAWIWGLVIPVVALLLSLISVKWGLLYLLIYPIQV
                     IRLAKHNSKSGKEAWMRAFFLVLGKFPEMVGQVKFFQRVCNGKQGSLIEYK"
     gene            complement(17785..18297)
                     /locus_tag="PKL42_02400"
     CDS             complement(17785..18297)
                     /locus_tag="PKL42_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HNU65950.1"
                     /translation="MFENIKADLKSYQGDFGAQGFWVMVVYRFGRWRYGVRPASLRKI
                     FSFIYKIMYKLMQILTGIELPCEADIGKNFIIDHFGGVVVSGYAKLGDNCRIRNGVVI
                     GLKNVEEVGAPVIGNNVDIGAGAKVLGKITIGNNVLIGANAVVISNVPDNSIAVGVPA
                     VIKARKLVQI"
     gene            complement(18360..19307)
                     /locus_tag="PKL42_02405"
     CDS             complement(18360..19307)
                     /locus_tag="PKL42_02405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNU65951.1"
                     /translation="MDISVILVSYNTIDLTKMALNDLFASVGDLELEVFMIDNASRDN
                     SVEVLRREYPNITLIENNKNVGFGRANNQALPFIKGRYVLLLNTDAFVEPDTLIKTMQ
                     YMDKHPRCGMVGVRLVGRDGVLQPSCRYFPTAWNIFLERTNLKKYFKHAKLIDNMSWD
                     HASVRSCDWVPGCYALIRKEVIDQVGLFDPRYFLYYEEVDHCFAAKRAGWEVAYYPDT
                     TVVHIGGESAKHEAAISSQSRQIESLQIESELLFFRKNHGVLGVITHLMLNSLADLIL
                     FLKALIKLKSPSDILFHLKRALFVWKMYFLTRFANVSTR"
BASE COUNT         5811 a         4509 c         3652 g         5441 t
ORIGIN      
        1 gtagacgtgt agttaaacga tacgtttttc caagtttcta gtgcctgcgc gagcggtttg
       61 caccatttac ctgcttcacg cagaatatct gggccctcat ttaaaatatc tttaccctca
      121 ttacgcgctt tcaccattac ctcaatacca acacgattag caattgcgcc tgcttgtatg
      181 ccatcaggat gaccaattgt gccaccacca aattgcagta ccatatcatc gccaaacaag
      241 tgtaaaagct gatgcatttg tcctgcatga atgccgcctg acgccactgg catgactttt
      301 cgtaagtctg cccaatcttg gtcaaagaaa ataccacgcg ataaatcctg tttcgtcact
      361 gcatcacgac atacgttgta atacccttgc acggtcatcg ggtcaccttc cagcttgccg
      421 actgctgttc cagcatgcaa gtggtcaacg cctgccaagc gtagccattt tgctatgacc
      481 ctaaacgata caccatgatt tttttggcgc gtgtaggtgc catgcccagc acgatgcata
      541 tgcaaaatca tgtcattttt acgcgcccaa ttgctgattg attgaatcgc tgtccaacct
      601 accaccaagt ctatcatcac aatattggaa cccaactgtt tggcaaattc tgcacgttcg
      661 tacatgtctt ccatggtggc cgctgtgata ttgagataat ggcctttaat ttcgcccgtg
      721 ttggcttggg cccgattaac ggcttccata cagaataaaa agcggtcacg ccaatgcata
      781 aatggttgac tattgatgtt ctcatcatct ttagtgaaat ccaagccgcc cgttaagcct
      841 tcatacacca cgcgcccgta atttttacca gataaaccta acttaggctt catggtggca
      901 cctaataacg gccgaccata tttatctaac cgttcgcgct caacgataat acccgttggc
      961 ggccccttaa aggttttcac atatgccact gggatacgaa tatcttctag ccgcgcggct
     1021 tttagcggct taaagctaaa tacattacca attaaagatg ctgtcacgtt tgcgattgac
     1081 ccttcttcaa acaaaatcaa atcataggca atgtaggcaa aatattgtgc ttctttgtcg
     1141 gtaccaaccc cagtgccagg cactgcatct atacgataag ccttaccctg ataatcttca
     1201 taggccgtta gcatgtctgt ccatacgact gtccacgtcg cggtggatga ttcgccagcc
     1261 acagctgcag ccgcttcttc tgggtctacc ccttcttgcg gcgtgatgcg aaatacgcag
     1321 ataaaatcgg tttcttttgg ctcataatct ggtcgccaat aacccatttg cttgtatttc
     1381 aacacaccac ccttgtagcg ctctttaata tcggtaatcc gtgcatctga ttgagtcgtc
     1441 attttttatc atccgctata aaaattaata agttgtaagt aatctggcta ttgcccacta
     1501 tatttaatat ataagttaag ttatattgat aaataattta ccatcaatta agttttactt
     1561 atgcgaaatt taacattacg gcaagtaaga atatttttaa gcgcgtacaa acacatgagc
     1621 tttgcacggg ctgcggaaga actttgtatt acggcccctg ctgtttctca gcaaattaaa
     1681 gaaatggaac aagacatggg cgtcaatatg tttattcgag aaaacagaaa aattgaactc
     1741 acttcagctg gcgaatattt tttgctctat gcgcgaaaaa tgctcagcac cgtgaatgaa
     1801 gcagacgtga tgatggagcg tctgcgtggt acacaaatga aaaccttgaa tattggcttg
     1861 gtgagtactg ccaagtattt tttaccgcat ttaatcgcag aatttaaaaa agactttcca
     1921 aatttaaaaa ttaaaatgga agcaagaaac cggcagcaac tggttgagtt attacgtgac
     1981 ggtgaaattg acatcgccat catggggcaa ccaccaaaag aaatggatac gcgtgtggaa
     2041 gcttttgcca aacatccgca tgcgtttatc gccaacgcgc aacatgcact tgcaggtaaa
     2101 ttgaacatca caccagatga gctcaattct tatgaagtaa tctcgaggga acttggctct
     2161 ggcacccgtt ttattatgga gaaatacttc actgagcacc aaatttcacc cattgtgagc
     2221 atgcagatat caagcattga agcgattaaa caggcagtga ttgctaattt aggcatcaca
     2281 ttcacctcgc ttcacaccgt aggaagtgaa atcagtaatc gccaattagt tgtactagat
     2341 gttcaagaaa cccccatcat gcggacttgg cacgtggtag ctttgaataa acacaacaac
     2401 tctcaggccg cggaagcttt cagatatttt atgctggaaa aaggtggaac actgcttaat
     2461 gagatgttta aaacgattgt aactcgtaac agttaagcta aattcgacga atgattaaat
     2521 tagctgattc aattgactga tttaatcaga catccacatt tttttgcaaa aaaatagctt
     2581 taaatttaat caaaacacgc ttaaaaacag ctcaaccttt tgacctgccc tggaagcctt
     2641 tatttacaag gctcgcagag cagtattttt cgctcgagag tgtttaatct attttaaacg
     2701 ttactttaaa ctacgttgtc gccgcgcttc atacaaacaa attccgctcg ccacactcac
     2761 attgaggctt tctactgagc caaacattgg aattgtcact aaggcatcac aattttctat
     2821 tgtaaggcga cgcaagccat ccccttcagc cccaagtaca atggcaatgg ggccatcaag
     2881 tttggtattg agcaaattac tgggtgcatc catggtggtg ccaaccacaa aaacacctgc
     2941 gtcttttagc tcgcggatgg ttctagctaa attcgttacc gcgataaaag gcacggtttc
     3001 agccgcgccg caggcgactt tacgcacggt tgcgtttagg ccgactgctc tatcctttgg
     3061 tgcaatcaca gcgtgcacac ccatcgcatc ggccacgcgc aagcatgcgc cgaggttatg
     3121 cgggtcttca acgccatcta aaattaaaaa aaacggcggc tctgtgaggt cagattctaa
     3181 aatatcgtga atatctttat acggaatcgg cgtatccacc acgcgcgcaa tcacgccttg
     3241 atgacggcca ttcatacctg ccatgccatc cagtcggcta cgttcaacct ccatggtgcg
     3301 cacacctgaa agttccgcca ttttcagcaa atctttcatg cgtgcatcca cgcggtcgcg
     3361 gtcaattaaa atttcttgca cactcgccgc atgctggcgc aaacggctca gcacggcatg
     3421 aaagccaaat aaaatacggg catcgctcat tgataatttt ccaagatgtt attcgggcat
     3481 tttagcatgg gtgagctaag ttaacttacc gatataggca tcatagcgat tcagtcacta
     3541 gatgattatt tagccttaaa tgacttgtaa attaaatata ttgctaagca ataaaatgct
     3601 agagcaatat ataacttacc tctttgatcc ccgaaaaata caaaaaaaat cgactcaatt
     3661 gcacatttac tacaaccaaa agtgagtaaa ggagtatctt taaatatata aactgttcca
     3721 gcaagagctg aagctaaaga tgcaaaaaaa aggaaaaaat taggttttcg cataccttta
     3781 aatatcaagc atgttttttc taatactaaa ataatctatc ttttcggttt gcccttattc
     3841 ttactctgcc tcacacggtc tttcttaacc gcttttttcg caggttttcc cgctccagtt
     3901 ttactacctt tcacagcttt ctcaggcaac ttcaatgtcg tctttttagg cacattttcg
     3961 ccagagggct ttttagaacc tttgtaatcc gcaccagtac tggcttgcag agcatcgcca
     4021 ttaatatctg ctgttttatt gattaaagta aagtcaattt tagtggtttc taaatctacc
     4081 cgcgcaactt taatggtgag cctatcaccc aagcgataac gcacacctgt gcgctcgcca
     4141 gccatttcat ggcgggcttt atcaaagtgg aaataatcat tgcctaactc ggtgacgtgc
     4201 aataggcctt ctacgtatac gccatctaac gccacaaata aaccaaagct agtcacgccg
     4261 gccacagtgc cttcaaacac ttcgccaatt ttatcttgca tgtaaaagca tttgagccaa
     4321 ttcgtcacat cgcgtgtggc atcatcggca cggcgctcgg tcatagagca ttgtgtacct
     4381 agtgcgttcc aatcacctgc tttgtatctt tcacctttta ataccgcttt aatggcgcga
     4441 tggataagta aatcggggta gcgacgaatg ggtgaggtga agtgcgcgta ggcttcataa
     4501 gccaagccaa aatggccgac gttatccggg ctgtaaacgg cttgttgcat cgagcgcaac
     4561 aatacggttt gcaacaactg agcgtcgggt cgttctctaa tacgtgccaa tagtttgcca
     4621 taatctttag catgcggctt gtcaccaccg cccacaccaa agccaaattc acccataaaa
     4681 gtacgcaaca actctaattt ttcaggggtt gggccttcgt ggatgcggta taaagctgga
     4741 tgctcatgcg ttagcagaaa atcggctgca caaacattgg ccgccagcat acattcttca
     4801 atcagtttat gtgcttcatt tctttggcta ggaataatgc tctcaacttt tccttgatca
     4861 ttaaacacca tgattgtttc tgaggtttca aactcaatgg ccccgcgttt ttctcgctgt
     4921 gtaagcataa gttgataaac gctattcaaa tgttgcaaat gcggcattaa ccaggcgtat
     4981 tcatgcgcca attcgccatc tggattttgc aacatttcgt atacttgtgt ataggtcatg
     5041 cgcgcttttg agcgcatcac tgaggggtaa aatttatatt gtttcaccac acccaagccg
     5101 tctacttgca tatcgcaaat catgcacagt cgctcaacat ctgggttaag cgagcacaat
     5161 ccatttgaca atgcttctgg caacatagga ataacacggc gtggaaaata aacagaattt
     5221 ccccgctcaa aagcgccttt gtctagggca tcacctggcc ttacataaaa actcacatct
     5281 gcaatggcaa cgaccaaacg ccagcctttg ccctgtggct ccgcgtacac agcatcatca
     5341 aagtcgcgcg cagtttcacc atcaatggta attaatggca attcgcgaca atcaatacga
     5401 tttttatagt ctgcggactg cactagtttt ggataggatt cagctaactt gacggcttcc
     5461 gcgctaaatt catgtggcaa attgtgcttg cgcagggcta tttcaatttc cataccacta
     5521 tcagcataat tgcccaaaat ttcaaccact ttacccatcg gctgggcatg caatgaaggc
     5581 tgcgaagtca actccaccat cacaacctga cctgctttag cacccatatc taaatggtaa
     5641 gggattagga tatctaaatt gatacgctta tcttcagccg ccacaatggt gacaccactg
     5701 gtttgcaata ctcgacccac caaacgttgc gtgcggtgct ctagcacttc gacaattttt
     5761 ccttctggtc ggccgcgcct atccaaacca gccatacgca ccatggcacg gtcgccgtgc
     5821 atcacttgcg acatttcttt agcgcttaaa aataaatctt ctccattgca tttactttta
     5881 tcgtctggaa tcaaaaaccc aaaaccatct gggtgccctt gcactacgcc tgcgattaaa
     5941 tccagtttat ccgcaataca caatgcacct tttcggtttt gaatgatttg tccttctcgc
     6001 tccatcgcat tcaaccgctt cgtaaaacta tcgcgttcgt cttgcccaat atcaagcagt
     6061 gtatagagtt gctctaaact taaaggcaca ccttgttcgg tcatgacaga caagatgtat
     6121 tcgcgacttg gcaaaggatg ttcgtatttg ccggattcac gctcaaaaaa cgggtctaat
     6181 tttcttttat tattcttctg gctgttcttt ttacttggcg acatggatta ccctttacga
     6241 tgaaactgtt ataatgcgca ctaccactta attggtagct caattttaat acgttttatc
     6301 ctgatatcat tcaatcacgc taaaacaaat aagcttacat gcccagatgg cggaattggt
     6361 agacgcgcta gtttcaggta ctagtatcga aagatgtgga gattcgagtt ctcttctggg
     6421 caccactgct acactataaa tcgaaattga atttaataaa aattttcggc cttttttaac
     6481 attaaattta tttactaact ctatccgtct acatttatcc cgaaagcaca atcaatctat
     6541 cacttgtcat caagattgcg cacttaaatg acaaaagtgt caaatttacg tcagtctaaa
     6601 atatccccca gtaaattttt tttgtatcta attgttttat atagttaata ttatccacta
     6661 ttcactggca ttcaacttgc aatagtcttt gtaaattgta attttgaaaa tgaatgggag
     6721 tgaatacaaa atggctacga ctttagaaaa caatcattgg gatacattaa ttgatgtcat
     6781 acaacgttct tataaaatta agaagcatgt tgattttttc agatggcaac aggatgatct
     6841 tgctaaaatt ttcccacatg atgtcttgat tgcggtgtgg ggcgacttca acaaaggtac
     6901 actaaactat gatgtaagct ctaacattcc gggtatacgt acgcaatcgc tgcgagaatc
     6961 atcagggcat attgatcacc taatgtcaga cctctaccaa aggtggcttg caaatagcga
     7021 acgctggtac agaattaaca acttctctgt tgactcagat gcagacggtg tgccagcttt
     7081 ttttaacaac cagctttctg tcattaactc acttctagtt tatggcgtgc gtgatattcg
     7141 tggtaataac gattgccttt acatcttcat ggacaaacag tcagagtttc caatcacatc
     7201 ttctgtgtta ggcatgttca tgcctcactt agatgccgcc cttcgtagaa tcgaaagttt
     7261 ggagtccgct gatgcagagg aagatgatct ttctgaatat cacgtacaag gtttaagcga
     7321 ccgcgaacat gagattattc attgggtacg aatgggtaaa accaactttg aaatcagtat
     7381 gattttgagc attagtccta atacagttaa aaatcactta aaacgtattt ttagtaaatt
     7441 agatgtttct tgccgtgcac aggctgtagc gacttatgtc ccaccaaaac tgaattaggt
     7501 catgagttta taaagctgtc ataataatgg attaattgaa aagtatttac ggcactacaa
     7561 atggcatata gacaatctgg ctcaaaaaca ctcttacaac ggcgtagtag tttaagtaat
     7621 attgttcaac aattactcga tggcgcagca gtgataggca ttgcatgggc gttaattatg
     7681 cgtcatatgg gcgcactata ctcaccttat atcaatttaa tattagtgtt actggttgtc
     7741 atggctttaa cttatgaccg atttgctatt tacagaagta atggtcatgc aatagacaaa
     7801 gcgtttaatt tattaaaagc atggacacta acttttttca tcttggttgt gctcgccttt
     7861 ttaacaaagc aaagcgaaat atattctcga aacttaatta cccagttata tatagcggga
     7921 tttattgcac agcttatcat ccacatgctc tttagattgg tctacaagaa catcgtacaa
     7981 catgctcagc aaaatgaaaa agttttgatt gtcgggcaaa gtcggctggc ttactatcta
     8041 caacacagaa tttcagataa cccttgggtt ggcgaacagg tgattggctt tgttaaatta
     8101 ttcgacgaag acaataaagc tagcggcaga aaagctgccg gcagaaaaaa cgaccctgct
     8161 gttcttggtg atgtgaacga tttacctgcg ttaattgatc tacatgacgt tagcacggtc
     8221 tatattgtca cgccgcttaa atcctcaatc gaacttgaaa agatttatat ggatgtcatt
     8281 gacaaacatg tcgccattca ttggattcca gacatttttg ctttaaggct tgtgaatcat
     8341 agtgttaaag agatttcagg cattcctgta ttaacacttt ctgaaacacc tcttattggc
     8401 atgcgtctac tgctaaaatc tatcgtcgat aaagtgctgt cagttttaat attgttgatg
     8461 attttcccaa tcctgatttt tgtggcaatt gcggtaaaaa tagacagtcc aggcccggtt
     8521 tttttcagac aaaatcgtgc aggctggagc ggaaagtctt tcagaatttg gaagttccgc
     8581 agtatgtacg tacaccagga agaatctggc aagttaaaac aagcatctaa aggcgatagt
     8641 agagttaccc gcgttggggc atttattcgc aaaacaagcc tagatgaatt gccacagatt
     8701 ttcaacgtac tcactggcga catgtcacta gttggccctc gtcctcatgc gcttcaacat
     8761 gacaaagaat attctcaaag aatttttgac tatttcgcaa gacaccacat caagcctggc
     8821 ataactggat tagcccaagt gagaggttta aggggcgaaa cggttgatat tgagcaaatg
     8881 gtgcaacgta ttgagtcaga tattgaatac attaataact ggtcaatttg gttggatatt
     8941 tccatcttat ttcgcaccgt ttttgccttt accggaaaag gtgcttatta gtcaacttaa
     9001 aagcaaggta tgcacgcaga attcaaatga taaaaattgt tacctgattt atttaacaac
     9061 caagtatccg tataaacaac cttcgaccct acaactaagc aattaatatc agcatgagtt
     9121 cctcactaaa atctatcaaa ccaatactgc gagatttgct tgctgaaggt ctatttatat
     9181 caagcatcag caagccaagc agatgtgcta acaataaatt gacgatagtt acttttcatc
     9241 gtgttttgcc agaggagcta cgacaaaaat atccttaccc aggtatttgc gtcacacccg
     9301 aagaattacg ttggttctgt aacttcttta atcaattctt tactgttggc tcactcacat
     9361 atcttcacca tcgattaatg aatggtgagc atccagaaaa accatattta gcaattacgt
     9421 ttgatgacgg gcaacttgac aaccttcaat atggattgcc agttttagat gaattcaatt
     9481 tcaaagcaac attttacatc ccagcacgca atgttgatga agctaatatt ctttggcatg
     9541 accgtatcgg ctactgcaca ctcaacgcca aaaacagtgg ttctgaagcc atgaaaaaag
     9601 tactgatact agcagaagct tacggggttc catcaaaagt aacggactca tttgaaagtc
     9661 atattattca aaattctaaa aaaatcgccc cagaaaaaag aaccgagttt attgaaaatt
     9721 tagaaaatat attaaaagta acaacgccag cgtggggcag gctcatgact tggagtgaaa
     9781 tacgtcaaat tcatcaacaa ggccatgaaa ttggctcaca ttcaatgaca catgcgctga
     9841 tgcctcaatg cgttgaccaa gatttaaaat atgaaattgt tgaatctaga aaacgactgg
     9901 aatcagagtt gaatgctgac atcacttcgt tttgttatcc taacggtgat tatgacgagc
     9961 gtgctatcga gacggtattg caagcaggtt atcggaatgc cgtgaccaca aaatggggta
    10021 aaaacaaacc aaatcaacct caatttcaac ttgatcgttg cgacatcaat acttttaata
    10081 attggaatcg taaacaagaa ctctctgtag cacgttttgc atttcggtta agtggcttac
    10141 agcccggcct ttaagttagt tacttaatga attttgaagg attagtgcag tgaatttagc
    10201 ttacttttgc agtgaatacc catcccgctc acacactttt attcgtcgag aaatccacga
    10261 attgcgccgt cgtggcgcga acattgacgt ttacgcacta cgtaaaccaa agttagaaaa
    10321 cctgatttca gatactgatc accaagaatt taaacaaacc atcagtattt tacccattaa
    10381 gccttggcag ttagtgttgg ttcacgggaa aatgttcttt aaaaacccac tcgcttattt
    10441 taaagctttt aaagtagcct ttggtcacca tcttcctggt gttaaaaata cattgtggtc
    10501 agtatttcaa tttgcagaag gaattgtact tgcacatcat cttaaacaaa acccagttca
    10561 acatttgcat attcattttg ccaactctgg cgccaatgtc ggaatgattg ccagtgaata
    10621 tttacagctg aactggtcca tgactctgca tggtacttct tgctttgatt acccttgggg
    10681 tcccctactt ggccaaaaac ttgaaaaatg tacgtttgcg aattgcattt cgtatttcgg
    10741 tctatcgcaa gcatttcgag cagttggtcc tcaacattgg gataaactgt ttgtatcaag
    10801 atgcgggatc gaattggaag ctgtcaatct ggcgaagtct aaacagtcaa gccaaccaaa
    10861 agatgcaact acgatacgct taatttctgt tggtcgactg catgtagaaa aaggctatcc
    10921 agtgatgatc agtgcctttg ccgaagcact aaaaacagtg cctaatatgg agcttgtgct
    10981 tttaggcgat gggccttatc gaattgcgct tgaaacttat gtgaaagaaa aaggcgtgtc
    11041 tgacaaagtc attttctgcg gtgccgtgcc tgaacaaact gtacttgatg aaatggtcca
    11101 tgccgatatt tttgcacttt cttcatttat ggaaggcatt cccatggtgc tcatggaagc
    11161 catgattctt gaagtacctg tgatcgcgcc gcgccttgcc ggcatcccag aattagtcat
    11221 tgaccacgaa aatggcttgt tgtttgaccc cgcgcacgca tcacaaatgg ctgaacatat
    11281 cgtaaccctt gcctcagata aagaaaaacg cgcacgcttt ggcgctacag gtaagcaaaa
    11341 agtgctggcg gaattcacga ttgaaaaagg cgtagaaaac ttgtggcaac gctttttaaa
    11401 aatcgagaaa aactaaacga aaaatctccg agagaaaatt tctgccctgt aaaccttata
    11461 aataaaggct tacaaaggag cataaaagaa gatgggtaaa atttaacgta aaaagtgagt
    11521 gtgttaccca acttgtagat gcgtattaag aagcgttata gcctaatgga ttgttgaatt
    11581 gccagcgcca tgtatcgcga cacatcgcat caatatcaaa cttggcttgc cagccaaaca
    11641 atgacttcgc caactgtgga tttgcataac acgttgcaat atcaccatca cgacgctcaa
    11701 caatgcgata tttcaccggc ttaccgcttg cagtttcaaa cgctttcacc agttgtaata
    11761 cgctacaacc tttgccagta cccagattca ccgtgactaa tccagtttgc ttttcaagtg
    11821 tacgcagcgc ggctaaatga cctagcgcta aatcaaccac atgaatatag tcacgcacgc
    11881 cagtaccatc tactgttggg taatctgaac caaaaacact gagctcctca aggcgaccaa
    11941 cggcaacttg cgaaatgtaa ggcatgagat tattgggaat accattaggg tcttcgccaa
    12001 tttggccgct ttcatgcgcg cctacagggt taaagtaacg caaaattgtg atcttccatg
    12061 tcggatctgc aattgaaagg tcccgataga tttgctcaac catcaacttg gattgaccat
    12121 aagggttaat cggtttaact ggaaaatctt caggaatttg taggtcaccc ggcgcatcat
    12181 aaacggtcgc tgaagagcta aagacagcac ttttaacgcc gaattctgac atcacttcag
    12241 ccagtgcaat actgcccgat acattgttat cgtagtattg caaaggtaac cgaatggact
    12301 caccaacagc tttatgccct gcaaaatgga taactgccgt gatggtatgt tgactaaaaa
    12361 cttcgcgcag aaagtttcta tcccgcacat caccttgttt aaatttgagt gcacggcctg
    12421 tgagttgctg aacacgcttc agcgattcaa atttgctatt gctaaaattg tctataacca
    12481 cgacatcaaa accagcattt aacaactcta agcaggtatg tgagccaata taacccgctc
    12541 cgccagtcac tagtacactc atgcgttcac tatccgaaat gtcaatatta aagtgcgttg
    12601 aaattaattg taagcaagga cgccaaattc agctctgtct cgcaatactt cttcaaccaa
    12661 gtcaacgcca attttctcta cgccacttga gaaaccatac actagcacca tgtcgcataa
    12721 aatattgata cttctaggca cacctttact ggcttctgca atcaactcaa tagcacgagg
    12781 ggtaaataaa cgtttttcgc aacccgccac ttttaaacga tgctctatgt atttcgagac
    12841 ttcagctact tgtaaaggtg ggatgaaaaa gtctacggca acacgctgaa agaattgctg
    12901 caaatcaggt ctacgcagta actctcttaa ttgcggctga cctacaagca cgacctgtaa
    12961 cagttggtct ttatctgcat tgatgtttga caacatgcgc aaagactcaa gtgcactagg
    13021 gctcaagttt tgcgcttcat caacaatcaa caccactctg cgtcctgccg tatagctttc
    13081 aatcaagaat ttctggaaag cttcatagag tgcgatttca gacatacctt cataaggttg
    13141 atcgaatgcc agcaaaatcc agcctaacaa atcatcaact ctttgatgtg tgttataaac
    13201 gataccgact gtaatatcct cattcacatt attaagcagg tgacgcacca gcgttgtctt
    13261 gccacagcca atatcaccac acaccaccgt aaagcctgaa cgattggtga tgccgtattc
    13321 aagcatagaa tacgctaagc tgtggcgttg accaaagtaa agaaaatcag gatcaggttg
    13381 aatagaaaac ggtttttccg tcaacccata aaacgcttca tacatactgg gaactacctt
    13441 tttacttaaa atgttgatta tttagtcttt aattaggtag tattctacca gcaaagtgat
    13501 gattttttca ttcactcaat cgtgtcaaac tgcaataaag cactaatcat gtgatttatt
    13561 gcgtgaggcg acaactcgat gcatgcgtaa catacgatat ataaatgcag ggttcccaag
    13621 tacatagcgc ctataaagcc gcttgggctc atgcactaac cgataaaacc actcaaaacc
    13681 gatttttcta atccattttg gggcgcgctt tactttattc cccatgaaat caataatcgc
    13741 accgccacag accaccaatg tcgcaatatt ctgatcacgt atcatagcgg ctattttttc
    13801 ttgttttggc atccccatgc ccagcacgat aaattcaggt tgcgtttcaa tcgccagatc
    13861 cacataaaac tgctctgatt caaacccatg atggacagaa actggaatga cgccatataa
    13921 agcgacagct tttgtaactg cttgactcaa ataaggctct tctgttcccc aaaaggccac
    13981 tcgcttgcca cggtatgctg ccaatagttt ggggatgaag tctgtaccat tcatatttaa
    14041 acccttattc acagcaagtc gcgtcattaa catcgacata cctgacccat ctctgagcaa
    14101 aatgtcagcg ccatgtaaag cgttatagaa cacttcattg gttaccattg agttcattgc
    14161 gaacgaattc acaaaaccca gaatccgtgg ataggtacta gtggacaact ccttaaccag
    14221 agcatcttcc tcaacaccat cggcgatgac gcgaatttta tcgaccattg cataccaacg
    14281 aattttccac tctactattt cattcatcat tttctcgaat cattcatctc gtttagcacg
    14341 tacccaattc acttgtctct ttacaaaaaa attcaggtaa acaggtacct taagaagcgc
    14401 ataaattggg gcatagcaca aactagccaa agaaatcact tgttggccaa atcttaccca
    14461 ggcaacaagt accgctatac ccaacaagag tagtccaacg ccagatagaa ctaatggcaa
    14521 cagcaaccca ctgacaatat aaaaaactgt gctgagtata aaaaccccca caatcaataa
    14581 gctcaataac gctaacggcg gcacaatcaa atctagcgcc aacaccaaaa gattcaaatt
    14641 aagcttgcgt atcgcctctg caaacagact tggcgcttca cttaatatca caccgagatg
    14701 tccatgctcc cagcgagtac gctgccctgt caaaccttcg gtgttggttg gaaacacact
    14761 ggtcactaac gcgtttttta aaaatagtgg cggcttatct gcctgactaa attcaatacc
    14821 taatttaaga tcctcaacaa tatggccact cgctaaattg gctttgctga tgccctccca
    14881 agtaaacgcc attccagtac ccattagctg gcacggcaag cccaatgcgg cgtaaccgga
    14941 tggcctgact agatttttca ccaaccaggc aaattcagca attttagttt tcaatcctgc
    15001 cccaactgga gatttcatta aatataaggc ttgtactggt cgaccaaact caatgcaagt
    15061 tgtagccagc ttgcctagcg catttggttc cacctgacaa tctgcatcga caatcacaat
    15121 cacttctggc ggagtttcgg cacaatggcg taccccaaaa tctagcgcat agcccttacc
    15181 tcgcgcgtct aaattgaaac gctcgataac ctctgcaccc gcatccctag caattttggc
    15241 agtgtcatca ttgcaattat cagctacgac aattaaacgg tcttttgagg tgagttgtgg
    15301 caatattgag gcgatggttg accgaatctg tacttgctca ttgtgcgctg gtacaaccac
    15361 agcaaatgaa ggccttgcga cggtgacaac cgtctctttc agtttcagta gtgatgaaaa
    15421 aatcacttgc gtaaaaaata caacaactgg caataacaat accgtcccca atacgatgag
    15481 caacacacca aaaaatagtg tcatgttgcg cccctttcac ataaacttaa atgttgcagc
    15541 ctatcaaact cttgtgagcc aggcacggta aaactatatt tcaacccacc caattcacga
    15601 gccaacatca caacttcggt agagttggtg ccaaaatgcg catgtatgtg cctagagcga
    15661 tgagaattta accaaggcaa gatacgacag gcctctgcta aaaaaaccag ataataaggt
    15721 aatgaccttt cagaaatcca gcccattttg aaagccagca tgagtgcgaa aaaaaatcga
    15781 attgggttca tcaacatctc gttgagcata ggtcttaaca atgggactaa gccatgctgt
    15841 agcacatatt gcgtacactt aagttcacga atatcaacct catctacagc gtctccctcc
    15901 cagtcaggaa cgcgatacgc tgaacataat aaccttgtcg ctctagcaca agaatttcgc
    15961 ggcgtataag tgtatagcta acttttggat acttattaat aaaataacta atgttcattt
    16021 aaaactaaaa tctactagga ataattaatg agaaacattc tggttttata ttacaaatga
    16081 cagagaagca aaattcacac caaattaaat attcatgcga atcataaagt tatattttta
    16141 tattgattta tcctgtcgat tattcggcat tagcgttaat ttaatggcat taaagtcgca
    16201 tctacaatat ttgaacgcca atagattggt tcaagattcg tctatcacct ttgctggaat
    16261 tcccactgca cttttgcctg caggaatatg tttaagcact actgcgtttg caccaataca
    16321 tgcatgattt ccaatagtta cgtttcctag tattttagcg ccagcaccaa tatccacatg
    16381 accgccaatt gtcggtggcg gactaccagc acgcatacct attgtaacct gttgaaaaat
    16441 taaacaattt ggaccaatga cggcatctgg gtgtatcacg atgccattag gatgaggtaa
    16501 cagcaaacct ccaccaagtt tactagttaa tggaatatcg gcacctgtca ccacactcca
    16561 aaaacggtgc cttaacacca tcaacttact catcaacttg aatagtggat tatgagaaac
    16621 tgaataacgc tgataatacc taatactggc caataatgac cttgctggat cccaagataa
    16681 aaaagtcttc tgctcacgtg accagtccgg gatttccgct gaaaccatgt cttgttgggg
    16741 agttgttgta ctcaatttaa tattctcggc accaataaaa gttacttata ttcaattaaa
    16801 ctgccttgct tgccgttgca aacacgttgg aaaaatttga cctgtccaac catttctggg
    16861 aattttccta agaccagaaa aaacgccctc atccaagctt ctttccctga cttgctgtta
    16921 tgcttagcaa gtcgaattac ttgaattgga taaatgagta aatacaaaag accccactta
    16981 acactaatca agctgagtaa tagcgcaacc acaggaataa ccaaccccca tatccaggca
    17041 cgctttgact cttgtaccca atgccgctct ggcggagcac catgcaaata agcgccttct
    17101 gcaaaagcgt ggcctgcacg catggtacgc ttccaccact ggccaaatgt tgtcattgcc
    17161 gcatcgtgta atgtcatttc ctgttcgaga cgccatactt tccaacccgc ggcgcgcaac
    17221 ctcacacaca attcaggctc ttctcctgca attaaacctg ctcgaaaacc atgaacagat
    17281 tcaaaggcgt tagcacgcat taatacatca cctccacacg cctttgcctc tccaataggc
    17341 gagtcccact caatgtcaca taatttgtta taaactgttt tgttaggaaa tcgttcacga
    17401 cggcgaccac aaacggcagc gacttgtgct tgactgtcta aaaaaccagt tgcacactcc
    17461 agccactgtg cattaacttc acagtcaccg tctacaaact gtacgaattc aatgtgtgga
    17521 aaaagctttc tcagccgagc gtaaccttca tttctggcac gtgcggcagt gaagggctgc
    17581 gacatatcta atgccacgat gtctgcacct aactcagttg caacactgat agaactgtct
    17641 gttgagcctg aatccacata caccatttga ttagtatgtt gcactaatga ttttaggcag
    17701 cgtacgagcc gttcgccctc attgcgacca ataattacca caccaatgcg gctagaaata
    17761 ctcattttga attactctaa tcatctaaat ttgaaccagc tttctcgcct taatcacagc
    17821 tggaacccca actgcgattg agttatccgg cacattgcta atcaccacag cattggcgcc
    17881 aatgagtaca ttattaccta tcgtaatttt gcctaacact tttgcgcccg caccaatatc
    17941 aacattattg ccaatcactg gtgcaccgac ttcttcaacg tttttaagcc cgatcacaac
    18001 accattgcga attcgacaat tatctcccaa cttggcgtag ccactaacca caactccgcc
    18061 aaagtgatca ataataaaat tcttgccgat gtctgcttca caaggcaatt caataccagt
    18121 aagaatctgc atcaatttgt acatgatttt atagataaac gaaaaaattt tacgtagtga
    18181 agctggacgt acgccatagc gccaacgacc aaagcgataa accaccatca cccaaaagcc
    18241 ctgcgcgcca aaatcgcctt gataggattt caaatctgct ttgatatttt caaacattgt
    18301 attattagca ataaagatgg atagttaaat cttgtaaaga tgttaatgag aattaatttc
    18361 taacgggttg acacattggc aaaacgtgta aggaaataca tcttccatac aaataaagca
    18421 cgttttaaat gaaagagaat gtctgaaggt gactttaatt tgataagcgc cttcaaaaac
    18481 aaaataaggt ccgcaaggct attcaacatt aaatgtgtaa tcacacccaa cacaccatga
    18541 ttcttgcgga aaaatagcag ttcactctct atttgcagcg attcaatttg acggctttga
    18601 gaagaaatcg ccgcttcatg ttttgcgctt tctcctccaa tatgcacaac cgtagtgtct
    18661 ggataataag ccacttccca acctgcccgt ttggcggcaa agcaatggtc aacttcttca
    18721 tagtacaaga aataacgggg atcaaataaa ccgacctgat caatcacttc tttacgaatt
    18781 aacgcatagc accctggcac ccagtcacaa ctacgcaccg atgcatgatc ccatgacata
    18841 ttgtcaatca acttggcatg cttaaaatac ttcttaagat tggtgcgctc caagaatata
    18901 ttccaagccg ttggaaagta ccggcaagaa ggctgtaaaa caccatcacg cccaaccaat
    18961 ctaacaccca ccatgccaca tcttggatgc ttatccatgt attgcatggt cttgattaat
    19021 gtatctggtt ctacaaaagc gtcagtattt aatagcaaca catagcgacc cttgataaag
    19081 ggtaaagctt gattgttcgc acgaccaaaa cccacattct tgttattctc aattaacgtg
    19141 atgttagggt actcgcgacg caacacttcg acggaattat cccgtgaagc gttatcaatc
    19201 ataaaaactt ccaactcaag atcgcccact gaagcaaaaa ggtcgttaag cgccatttta
    19261 gtcaaatcaa tggtgttgta actaaccaga attactgaaa tatccaactt tagccttcta
    19321 tctattacct ttaaatcaca gtcatctttg tctgtgcatt ttcaagagcg tatttaatag
    19381 gattacagtt caattatcaa gagaaaccat gct
//
LOCUS       DAOQSI010000018        18996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_2033_length_18996_cov_6.069057, whole genome shotgun sequence.
ACCESSION   DAOQSI010000018 DAOQSI010000000
VERSION     DAOQSI010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 18996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18996
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2033_length_18996_cov_6.069057"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="PKL42_02410"
     CDS             complement(<1..1116)
                     /locus_tag="PKL42_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNU65952.1"
                     /translation="MTEASETPVKSRFNLSEWALKNQVLVLYVMLMLTISGMLSYSKL
                     GQSEDPPFAFKVMLVRTTWPGASAVEVEQQITDKLEKKLQEVPNLDYSSSYSRPGESL
                     IFIVIKDSTFSEAIPDIWYQVRKKITDIRHTLPHNIESLTFNDEFSDVYGSMYALTGD
                     GFDNFALKKQAETIRAELLKTPDVAKVEFFGERKQRIFLEISNAKLSTLGISTTELIN
                     ILQTQNAIVRGGTFDSPQERIRIDVSGRYNTLEDLREIRLRANNQDFRLADVARVYRG
                     YEDPPHDTVRYEGQETLLLGVSMREGGDVIALGQHLAEKLAKIQQNLPVGLSLNTVTS
                     QPKIVANSVSDFVKALVEALVIVLGVSLLSLGLRTGVV"
     gene            complement(1128..2201)
                     /locus_tag="PKL42_02415"
     CDS             complement(1128..2201)
                     /locus_tag="PKL42_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNU65953.1"
                     /translation="MNPTKSSIIMALSSLLLACERPVEPPLPPRPALVTIVGSSAANS
                     SMVLVGEVKSRYETNLGFRINGKIIERNVEVGSVVKKGQVLARLDASDTNLSNQAANA
                     DVRAAEANHALAKAEVERQRLLFNKKFISQSALEIREAELKTSAARLQQVKAQAAVSS
                     NQSRYTALVADRDGVVTKIHAEPGQVVEAGSTIAQIVDTKQIEVLVAVPESRMTQLKV
                     GDAVAVKLWAAQSKTYQGKVREIAPAANSATRAFDVRVTIANADEAVKLGMTAGVNFG
                     ESQPDEIIIPSTALTQIEGKTSVWVINQAGIVQPRAVTAGAYSEAGIVISSGLQTGEM
                     IAIAGVHTLTKGQQVKPKVEAAL"
     gene            complement(2228..2857)
                     /locus_tag="PKL42_02420"
     CDS             complement(2228..2857)
                     /locus_tag="PKL42_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNU65954.1"
                     /translation="MPPKPKTEAQRQQLRTLIIDAARDLFVSKGVEAVTMREIAKRIG
                     YSATSIYLHFTDKESVLRAICETDFLALATSQKNIFEIADPVERLMMLGRGYAEFALS
                     HPNHYRLMFMTKHLPRHPNDLALEQNHPEQDAYFQLQLVVNDVFKAGLFKPALQDADL
                     IAQTIWAGMHGVCALEITMAADTWVNWRDIASRLQMMQSVLISGLLKSN"
     gene            complement(2999..3469)
                     /locus_tag="PKL42_02425"
     CDS             complement(2999..3469)
                     /locus_tag="PKL42_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HNU65955.1"
                     /translation="MNDTYLWLKTIHILGVVLFLGNIIITGWWKVMADNTKNAQVIAF
                     AQRQVTLTDYVFTAGGAAILLLAGFANVGIHQMSFSAKWLSQGLWMFVLSGIIWLVVL
                     IPTQVKQAKMAREFSQTNVIPETYWKLCTRWNIYGAIAVLLPLINLYWMTFKPA"
     gene            complement(3486..3932)
                     /locus_tag="PKL42_02430"
     CDS             complement(3486..3932)
                     /locus_tag="PKL42_02430"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNU65956.1"
                     /translation="MIVKKLVCDSFRQLSAGNYLSAYNLMAEDCHYHFIGNHALGGQR
                     YNRKLIALWFQRFLRILPDFQFIPANIIVSGWPWNTTVAVKLHVSWQRPDGKTYQNIA
                     LQMMKLQWGKAVDIITIDDTRALGDLLEDVSQQFGTAEASAKPIEG"
     gene            complement(4043..5440)
                     /locus_tag="PKL42_02435"
     CDS             complement(4043..5440)
                     /locus_tag="PKL42_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNU65957.1"
                     /translation="MDKQKTILAVDDEPSMRRLLEISLRQAGYQPVVAADGKEALQMI
                     RNQTIDLVVSDLHMPSMNGLELLKNIRLENETLPFIMVTAQGEIKTAVEAMKLGASDY
                     ILRPFDLETLEIAIAKALSVKRLNLENTYLKSNTNTENDGLVGLSAPMMALKQLIRQV
                     APEKATVLITGETGTGKELVAKAIHQHSDRKNALFVAVNCAAIPAEMLESELFGYEKG
                     AFTGAVKDRMGKFELAEGGTIFLDEITEMPINLQAKLLRALQEGVIEKLGGNRQITLD
                     IRVIAATNRDPLQAVKEGKLREDLYYRLNVFQLQIPALRERGDDIGLITQDYLAKKAA
                     QISDEALQKLNAYTWPGNVRELQNVLERAAILAGGKPITSQHLPADLVSSAPLSDIQP
                     SASLSAESANSNASPVMQMATQGFSIPQTIAQIERQLIVDAIAACKGNKSKAAKLLEI
                     SERSLWYKLEQYQLK"
     gene            complement(5462..7132)
                     /locus_tag="PKL42_02440"
     CDS             complement(5462..7132)
                     /locus_tag="PKL42_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNU65958.1"
                     /translation="MLKKPLSIRQLLLLAFLLAGLLPAMLVSFLSFYQARIALKSEIS
                     HDLQTLSNTVASNVESMLFERFHNVHSWSELAIMQDIQIGDIDKRLATFLQELVISYG
                     GIYQKIEVVDTNNVIVASSVPKNLGLTTQPFATWFQVNIGQKTIDVYPIETHQTPSLA
                     ISKAILSPDAGQVQGRLIAYFNWQLVLDKLNQSVQDSTAAALFDEQNHAIASTRNWAK
                     IHAEHGMHANSTFSGSTSPKWRVEIEKLHSVAVAPVHRLGYVFLALLITTLVFATFLV
                     TPIAKAVTAPLSQLTHFVRNFFLDQTSQLPTSGPAEVQELSSAFKKMISELESSQKNL
                     ERAAKLAVVGEMAAAMSHEVRTPLGILRSSANVLQREPQLSKEGHEVLGFIISETERL
                     NNLVLSLIDAARPRLPAFTEVNLSALAIKCIAMLSAQAQTKNVQLDCHADQDYLIKAD
                     SEQMTQVLMNLLINAIQMLPNHGKVEVGITALQDTIQLTISDNGPGIPAGSQAQIFEP
                     FFTQRAGGVGLGLAVVRQIVHAHQGEISYSPSQQGGAQFTITLPKLTP"
     gene            7190..8203
                     /locus_tag="PKL42_02445"
     CDS             7190..8203
                     /locus_tag="PKL42_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65959.1"
                     /translation="MNKRLIMIFASVTSLLLLCKSAWAEDEISSDAVPATFIDGFSLG
                     GYSSASLVIPRNQSVEAAIDEISLILRWENDGRFKFFGELELERPISWNDNERISHKN
                     AYLDLERLYLDYNLSEKLNLRTGRFLTPAGRWNLLHAAPLVWTTTRPLVTSRLFPAST
                     NGLMLYGSVPMQNKAFEYTFFVETLKDQYKDDFEIQYRDVSGAHFSLNNEVNLGLTLM
                     TLRQTDPRSPAYRMIGLDFLTQLGRLELSGEGYYRFASNGKDGGSGAYVQSAYHLGNE
                     WYGLARLETFQSPQEGSAERWLVGATKRLKPNQLLKMEFTGGSGELPDAPRGFIGSFA
                     ILF"
     gene            8203..8673
                     /locus_tag="PKL42_02450"
     CDS             8203..8673
                     /locus_tag="PKL42_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65960.1"
                     /translation="MRKFLQFLMLITLAGLAQAEQARPIAVIMQNDEEASKLAAEELS
                     LIYWRKKQYWQGGLRIHPVNLHAEHPLRLNFSKVVLGSLPATQTNYWNGLYFHGTTPP
                     YSVQSEEAVIRYVANTKGAIGYVDACKVDARVNAVLWISDNKLSATKPALDCGS"
     gene            complement(8882..10822)
                     /locus_tag="PKL42_02455"
     CDS             complement(8882..10822)
                     /locus_tag="PKL42_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HNU65961.1"
                     /translation="MQTVSTQKPLRTLPLFGFLAIFALCTWLILRVVLALNIGLGAMQ
                     FGEILGAFAKGLWFDVAALAYLVVPWLISSILLPNRWRHLAWVNKARWLVAFLATFGL
                     IFGAVSEFIFWQEFTTRFNFIAVDYLIYTNEVIGNIRESYPVPLILTGIALLVAGLLF
                     IASRFVRFDNRPKTTKNKLALLATAIALPVLSYQTTNVDQMEFSKNAYANELSGNGLF
                     SFSAAARRNELDYDKFYRTIPQAQADAILASNGLARQPLSQALQSEPQIETKAIGPFK
                     RRPKNVVLISVESLSADYLGTYGNQENLTPTLDKIAKEGLVFDKIFATGTRTVRGLDA
                     LSLGTPPIPGQAIVHRPNNEHLATVGEFLEAQSFYTFFIYGGYGMFDNMNAYFRGNDY
                     KVIDRTDFDAKSIQAENVWGVDDESLFNNAIKAIDDNVKNATPFFAHIMTTSNHRPFT
                     FPEGKIDLPQGKRAGAVKYTDYAIGKLIENAKTKPWFNDTLFIIVADHCSSAAGKTKL
                     PVDKYHIPLIFYAPDMLPAGHYPRMASQIDIAPTVMHLLGAKGEQHFFGENLFKPAEN
                     QTAPRAFLSNYQELGYYKNDTLIVLSPKQKAEAFRVNPKTLESTPASMNETLLREAIA
                     YYQTASRAYKTGALNESENLNL"
     gene            complement(10890..11600)
                     /locus_tag="PKL42_02460"
     CDS             complement(10890..11600)
                     /locus_tag="PKL42_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNU65962.1"
                     /translation="MQFQKNHAKAQTSAFWLRSGVWTLAFALFMMLIYPTFDLDFHLS
                     NLFFDAQQQRFTLRGHPILNVWLHTGIKWVMVCVALVSLALAISAHWLVKLKPYQSAL
                     FWVFIGLVLSTSAVAILKHDSMHACPWDLTIYGGDLPFFDLFKNPPASTKSGGCFPAG
                     HPSGGFALMAFYFAFRQYRARFAGAMLWLGIFMGLAMGLVQIIRGAHFLSHVLWSGWV
                     VWLTLLLLYGLWTPKIKS"
     gene            11990..12655
                     /locus_tag="PKL42_02465"
     CDS             11990..12655
                     /locus_tag="PKL42_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermostable hemolysin"
                     /protein_id="HNU65963.1"
                     /translation="MKIYKMITSPAFSLSTAAFFHAEAAYQHRALAAAKRHRINSITV
                     ATSDEYAPDRHELEQFVHDVFAKTYGADVQQFMPELVSLRDEHGDLVAAFGLRKATNS
                     ALFLERYLDAPVETVLSQRFNKPITRTAITEIGNLAVANPRHAGILIAHVIGHSLDIG
                     VEWAVATAHHTLQNGLIKGGRDVYALQAADKNRLSTAEQKTWGTYYDNAPQVVAIKGI
                     AQI"
     gene            12652..14151
                     /locus_tag="PKL42_02470"
     CDS             12652..14151
                     /locus_tag="PKL42_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNU65964.1"
                     /translation="MSAANSPPLLAAIWQHADRQPNRVALQSLQKTVTYAELASAIAQ
                     AQAILSLQTKQATIAVALENQPAWVVLDLAAITNGNPLVPIPAFFSAAQMRHAVSDAG
                     ATTLITDNPSNLQSIFAEIIDATSTIEVASISLTLFILKSEGKALPQQTAKITYTSGT
                     TGNPKGACLSLAAMTNVAQAIQLATKLRAEDQHLCVLPLATLLENVAGVYAALLAGAT
                     IHLLPSDQVGLMGSKLNIMQLHLALIYTQASTAIFIPEMLQALVQIVESGAAKLPSLR
                     FLAVGGASVSPQLLDRAAKIGLPVFEGYGLTESASVVSLNTPEANKMGSAGKPLSHIE
                     IKITGQHEVLVKGANFLGYTNQQQQIEQNAFIDTGDIGYLDDEGYLFINGRKKNVFIT
                     SFGRNVSPEWVERELKVSPYIAQAAVFGEARPWNVAVIVLRQPMDNIAISKVIQAVNA
                     YLPDYASVAQWIIADTPFTAANQQLTTNGKNRRDMIWQHYQDKINALYS"
     gene            14161..14832
                     /locus_tag="PKL42_02475"
     CDS             14161..14832
                     /locus_tag="PKL42_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HNU65965.1"
                     /translation="MTFYQKLLSATEKERNHLLSLPLITQGAKGQISLPTYVGFLTQA
                     YHHVKHTTPLLMACGGRLPGHYEWLRTAIGEYIEEEMGHQEWVLNDIAACGGDKETVR
                     HSKTAETSASTATEVMVAYAYDMIYRVNPVGFFGMVLVLEGTSTAVATQAGETLMNTL
                     NLPKKAFSYLLSHGSLDISHVSFYESLVNQITDPEDQAMLIHSAKVFYKLYGDIFKEI
                     ELRLM"
     gene            14855..15655
                     /locus_tag="PKL42_02480"
     CDS             14855..15655
                     /locus_tag="PKL42_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNU65966.1"
                     /translation="MQLKGKRILLTGATGGVGIVLAQQLALKGARLALVGRDLTKLNA
                     LKQSLVAQNGEHAVITANFDTAGTTQPLVDEAVKKLESIDILINNAGILDFIQLEDQP
                     EQRIAQMVQTNVTTLIQLTRALLPSFKAKNQGHFVMIGSIFGSLGFPHYATYCATKFA
                     VHGFSQALRRELVNTNIKVTYIAPRGIKTTMNDANTTAMMEQSGNKMDTPEIVASIII
                     KAIENEKQEVFIGQPQSFFAWLNGVLPMAVNFGLKKQTALAVPFLRKK"
     regulatory      15949..16061
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            16124..18004
                     /gene="thiC"
                     /locus_tag="PKL42_02485"
     CDS             16124..18004
                     /gene="thiC"
                     /locus_tag="PKL42_02485"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNU65967.1"
                     /translation="MHTVNANPKFLNTTAEVDPATTQPFAKSRKVYVEGSRPDIQVPF
                     REISLSDTPSAFGADKNPPVMVYDTSGPYTDPTAKIDIRSGLPALRAKWIEERNDTEQ
                     LDGPTSTFGQQRKVDPELAEMRFNLTRLPRRAKAGMNVSQMHYARKGIITPEMEYIAI
                     RENHRRENMSELLQTQHKGHDFGASIPKMITPEFVRDEVSRGRAIIPMNINHPEIEPM
                     IIGRNFLVKINANIGNSALGSSISEEVEKMVWSTRWGGDTVMDLSTGKNIHETREWII
                     RNSPVPIGTVPIYQALEKVDGKAEDLTWEIFRDTLIEQAEQGVDYFTIHAGVRLAYIP
                     MTAKRMTGIVSRGGSIMAKWCLAHHKESFLYEHFEDICQIMKQYDVSFSLGDGLRPGS
                     IYDANDEAQFAELKTLGELTQIAWKHDVQCMIEGPGHVPMHLIKENMDLQLEHCGEAP
                     FYTLGPLTTDIAPGYDHITSGIGAAMIGWYGCAMLCYVTPKEHLGLPDKEDVRVGIIT
                     YKIAAHAADLAKGHPGAQIRDNALSKARFEFRWEDQFNLGLDPEKAKEFHDETLPQEG
                     AKQAHFCSMCGPHFCSMKISQDVRDYAAEKGVSEQEALTKGMQEKAIEFVKKGSEVYQ
                     KV"
     gene            complement(18061..>18996)
                     /gene="gap"
                     /locus_tag="PKL42_02490"
     CDS             complement(18061..>18996)
                     /gene="gap"
                     /locus_tag="PKL42_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNU65968.1"
                     /translation="QEAEFSDIEIVAVNGTQELDYMVYMLKYDSVHGRFNADVRTENG
                     HLVVNGKKIHVTSERDPAYIDWDTSGVDVLVESTGKFLTQESCEVHLERGAKKVVQSA
                     PGKDDTPMFVYGVNHQDYKGQAIVSAASCTTNGLAPLVKVLNDNFSVKRGLMTTVHAA
                     TASQLTVDGTSKKDWRGGRGVFENIIPSSTGAAKAIGVLIPSLDKKITGMALRVPSAD
                     VSVVDLTVELNKEATYKQVCEAMKTAAQGELKNVLAYTEEKVVSTDFRTAPAASVFDA
                     DAGIMLDSTFLKVIGWYDNEYGYTCNLLRLAKHIA"
BASE COUNT         5264 a         4230 c         4285 g         5217 t
ORIGIN      
        1 caccacaccc gtgcgcaacc caagtgagag tagacttacg cccagcacaa tcactaacgc
       61 ttctaccagt gccttgacga agtcactgac tgaattggcg acaattttgg gttgtgaagt
      121 gactgtattt aggctcaacc caactggcag attctgctga attttggcaa gtttttcggc
      181 taaatgttga ccaagcgcaa tgacatcccc gccttcgcgc atgcttacac ccagcaataa
      241 cgtttcttgc ccttcgtagc gaacggtgtc gtgcggtggg tcttcataac cgcgataaac
      301 gcgggccaca tcagccagtc taaaatcttg attatttgcg cgcaatcgaa tttcacgcag
      361 gtcttctaag gtgttatatc tacccgaaac atcaatcctg atgcgctctt ggggcgaatc
      421 aaatgtaccg ccacgtacaa ttgcattttg tgtttgtaaa atgttaatca gttctgtggt
      481 gctgataccc aaggttgaaa gtttagcgtt agaaatttct aaaaaaatgc gctgttttcg
      541 ctcaccaaaa aactctactt tagcaacatc gggcgtttta agcagttcag cgcgaatggt
      601 ttcagcctgt tttttaaggg caaaattgtc aaaaccatcg cctgtgagtg catacatgct
      661 gccgtaaaca tcactaaact catcattaaa agtaaggctt tcaatgttgt gcggcaaggt
      721 gtgacgaata tccgtgattt ttttgcgtac ctggtaccaa atatcaggga ttgcttccga
      781 gaacgtgctg tctttaatga cgataaaaat gagcgattca ccggggcgag aataactgct
      841 ggaataatct aaattgggca cttcttgcag cttcttttca agcttatcgg taatttgctg
      901 ctcaacctca actgcacttg ctccaggcca agtggtgcgc accagcatca ccttaaaggc
      961 aaagggcggg tcttccgatt ggcctaattt actataagac agcatgccag aaatcgtcag
     1021 catcagcatg acatacagca ccagcacttg gtttttaagc gcccattctg ataaattaaa
     1081 acgcgatttt acgggtgttt cgctagcttc agtcatatcg ttattgatca caatgccgcc
     1141 tcaacttttg gcttaacttg ctgccctttc gttaaggtgt gcacgccggc aatggcaatc
     1201 atttcacctg tctgcaaacc gcttgaaatt acaataccgg cttctgaata agcccctgca
     1261 gtcaccgctc taggttgcac aatgcccgct tggttaataa cccaaacgct ggttttaccc
     1321 tcaatttgcg tgagtgcggt ggatggaata atgatttcat ctggttgtga ttcaccaaaa
     1381 tttacccctg ctgtcatgcc aagttttact gcttcatctg cattggcaat ggtgacgcgt
     1441 acatcaaagg ctctggtggc agaatttgcc gctggggcaa tttctcgaac ttttccttga
     1501 taggttttgc tttgtgcggc ccaaagtttt acagcaaccg catcccccac tttgagttgc
     1561 gtcatgcgtg actctggcac ggcaactaga acttcaattt gtttggtatc gacaatttgc
     1621 gcaatggtac tgcccgcctc taccacttgg ccaggttctg cgtggatttt tgtgacaacg
     1681 ccatcgcggt cagccactaa agcagtatag cgtgattggt tacttgaaac cgcggcttgc
     1741 gcttttactt gctgcaagcg cgcggctgaa gttttaagtt cagcttcacg aatctccagc
     1801 gctgattgag aaatgaattt tttattaaat agcaaacgct ggcgctcaac ttcagccttc
     1861 gcgagcgcgt ggttggcttc ggcggcgcgc acatcggcat tggcggcctg attgctcagg
     1921 ttggtgtcgg acgcatctag gcgcgccaac acttggcctt ttttgaccac actgccaacc
     1981 tctacattgc gctcaataat tttgccatta attctaaagc caaggttggt ttcataacgt
     2041 gactttactt cgcccaccag caccatgctg ctattggcgg cactgctgcc aacgatggtg
     2101 accaatgcgg gacgaggcgg tagcggcggc tctacggggc gctcacaggc tagcagcaag
     2161 ctgctgagcg ccatgattat gcttgatttt gttggattca tttttattct ctttttttac
     2221 aaaattttta atttgatttt aaaaggccgc taatcagcac cgactgcatc atttgcagac
     2281 gtgatgcaat atcgcgccaa ttcacccagg tatctgctgc catggtgatt tctagggcgc
     2341 aaacaccgtg cataccggcc caaatagttt gcgcaatcag gtctgcatct tgtagcgctg
     2401 gcttaaataa cccggcttta aatacatcat tcaccaccag ttgcagctga aaataagcat
     2461 cttgctcagg gtgattttgc tctaacgcca aatcatttgg gtggcgcggc aaatgtttag
     2521 tcataaacat caggcgataa tgattggggt gcgataacgc aaactctgcg taaccacggc
     2581 caagcatcat gaggcgctca acggggtcgg caatctcaaa aatattcttt tgcgaagtgg
     2641 caagtgctaa aaagtctgtt tcacagatgg cgcgcagcac agattcttta tcggtaaaat
     2701 gcagataaat actggtggca gaataaccaa tgcgcttggc aatttcacgc atcgtgactg
     2761 cttcgacgcc ttttgacaca aacaaatcgc gcgcagcatc aataattaag gtgcgtaatt
     2821 gttggcgctg ggcttcagtt tttggtttag gtggcatggc aattttgtat caaaaataat
     2881 gcttaaatat ttggttgttt tacatcaacc tttttcgatg ctctgcaagc cttataaaca
     2941 aaggctcgca gagcataaaa tttttctcgg ggctttttca tctattttac cgatattttt
     3001 aggcaggttt aaatgtcatc caatataagt taattaaagg caacagcaca gcaattgcgc
     3061 cataaatatt ccagcgtgta cagagtttcc agtaggtttc cggaatcaca ttggtttgag
     3121 aaaattcacg tgccattttg gcctgtttaa cttgggttgg aatgagcaca accagccaaa
     3181 taatgcctga taacacaaac atccataggc cttgcgataa ccattttgct gaaaaactca
     3241 tttggtgtat tccaacatta gcaaaacccg ctaataataa aatggcggca ccgcctgcgg
     3301 taaatacata atcggtcagt gtgacttgcc gctgggcaaa agcaatcacc tgtgcgttct
     3361 tggtgttatc tgccatcact ttccaccagc cggtgatgat gatgtttccc aaaaataaaa
     3421 ctactccaag tatgtggatt gttttgagcc ataagtaggt gtcattcatt attcgtccct
     3481 atctattatc cttcaatcgg cttagcgctg gcttcagcag tgccaaattg ttgtgagaca
     3541 tcttcaagta aatcacctaa tgcgcgcgtg tcatcaatcg tgataatgtc aacagctttg
     3601 ccccattgca acttcatcat ttgtaaggca atattttggt atgtttttcc atcggggcgc
     3661 tgccatgata cgtgcaattt cactgcgact gtcgtgttcc atggccaacc gctgacgatg
     3721 atgttagcgg ggataaattg aaaatcaggc aggatgcgta aaaatcgttg aaaccatagt
     3781 gcgatcagtt tacggttgta tctctgcccg cccagcgcat ggttgccaat aaagtgataa
     3841 tgacaatctt ctgccatcaa gttataggcc gagagataat tacccgcgct taattgacga
     3901 aaactatcac acactagctt tttgacaatc atgtgataca taacatactt aaatatttat
     3961 tattaacagc gttatattaa caatgttaac taaaggatgt caagctcaat ttatcgagtt
     4021 ttggttacct tccttattaa gattatttaa gctgatattg ctctaattta taccagagtg
     4081 agcgttcgct aatttctagt aattttgccg ctttgctttt attgcctttg catgcggcaa
     4141 tcgcgtccac aatcagctgg cgttcaattt gtgcgattgt ttggggaata gaaaaccctt
     4201 gtgttgccat ttgcattact ggagaagcat ttgagtttgc ggattcagcg cttaggctgg
     4261 ctgaaggctg aatgtcagaa agtggcgcgc ttgatacaag gtctgcgggt agatgttgcg
     4321 aggtgatggg tttaccgccc gctaaaattg cggcacgttc taatacattt tgcagttcgc
     4381 gcacattacc gggccaagtg taggcattta gtttttgtag ggcttcatcg cttatctgtg
     4441 cggccttttt agcgagataa tcttgggtaa tcaggccaat gtcatcaccg cgctcacgca
     4501 atgcggggat ttgcagttga aatacattca ggcggtaata caaatcttcg cgtagcttac
     4561 cttctttaac ggcttgaagt gggtcgcggt tggtagcggc aatcacgcga atatctaatg
     4621 taatctggcg gttgccacct aatttttcaa tcacgccttc ttgcagggcg cgcaaaagct
     4681 tggcttgcag attgatgggc atttcggtga tttcatctaa aaaaatggtg ccgccttcag
     4741 cgagttcaaa cttacccatg cggtctttca cagccccagt aaacgcgcct ttttcatagc
     4801 caaacaattc agattccagc atttctgcag ggattgccgc gcaattaacc gccacaaata
     4861 atgcattttt tctgtcagaa tgctggtgaa ttgctttggc caccaactct ttaccagtac
     4921 ccgtttcacc cgtaatcagc acggtggctt tttcaggcgc aacttggcgt atcaattgct
     4981 ttagcgccat catgggggca cttaggccta ctaaaccgtc attttcagta ttcgtgttgc
     5041 tttttaaata agtattttct aaattaagcc gtttaactga aagcgcttta gcaatggcaa
     5101 tttctagggt ttctaaatca aatgggcgca gaatataatc gctcgcacct agtttcatgg
     5161 cttcgaccgc cgttttaatt tcaccttgtg cagtcaccat aatgaagggt agcgtttcat
     5221 tctcaaggcg gatgtttttg agaagttcca gcccgttcat gctgggcatg tgtaaatcac
     5281 taacgactaa atcaatggtc tgatttctaa tcatctgcaa agcctctttg ccgtcagctg
     5341 ccacaaccgg ttgataccca gcctgacgca ggctaatttc aagcaaacgg cgcatgcttg
     5401 gctcatcatc caccgctaaa attgtttttt gcttatccat ctttgtgtgg tctcaatagt
     5461 atcaaggggt taatttaggt aaggtgatgg taaattgcgc accgccttgt tggctaggtg
     5521 aataagaaat ttcgccttga tgcgcatgca caatttgccg taccacggct aaacctaagc
     5581 ccacgccacc tgcgcgctgg gtaaaaaacg gctcaaaaat ctgtgcctga ctgcccgctg
     5641 gaatgcccgg gccattatcg gaaatggtga gctgtatggt gtcttgcaag gctgtgatac
     5701 cgacctccac cttaccatga ttaggcaaca tctgaattgc attgatgagc aggttcatca
     5761 gcacttgcgt catttgttcg ctatcggctt tgattaaata atcttggtct gcatggcagt
     5821 ccagctggac atttttagtt tgcgcctgcg ccgataacat tgcaatgcac ttgatcgcaa
     5881 gtgcagacaa attcacttcc gtaaatgcag gtaatcgtgg ccgtgcggca tcaatcaagc
     5941 ttaagactaa attgttcagg cgctcagttt cactgataat aaagcccaac acctcgtgac
     6001 cttctttact taattgcggc tcgcgctgca atacattggc tgacgaccgc aatatgccca
     6061 aaggcgtacg cacttcgtgg ctcattgcgg cggccatttc gccaaccacg gctaatttgg
     6121 ccgcacgctc aagatttttt tgtgatgatt ctagttctga aatcatcttc ttaaaggcgc
     6181 tagaaagctc ttgcacttca gcagggccag aggttggcaa ttggctggtt tgatcaagaa
     6241 aaaagttgcg tacaaaatgc gtgagctgcg atagcggcgc tgtcaccgct ttggcaatag
     6301 gcgtcactaa aaacgtggca aaaaccagtg tggtaataag taaggccaaa aaaacatagc
     6361 ctaaacgatg cacgggagca actgcgactg aatgcagttt ttcaatttct acacgccatt
     6421 ttggcgaagt gctgcctgaa aaagtactgt ttgcatgcat gccatgttcg gcatgaattt
     6481 tagcccaatt acgcgtgctg gctattgcat gattttgttc gtcaaacaat gccgcagcgg
     6541 ttgaatcttg taccgattga ttaagtttat ccagcactaa ttgccagtta aaataagcaa
     6601 tcaagcgccc ctgcacttgg ccggcatcag gcgagagaat agctttggaa atggctaagc
     6661 tcggagtttg atgagtttca atggggtaaa catcgatggt tttttgccca atattgactt
     6721 gaaaccaagt ggcaaaaggc tgtgttgtta agcctaaatt cttaggcaca ctggaagcga
     6781 caatcacatt gttggtatca accacttcaa ttttttgata aataccgcca taactaatca
     6841 ccagttcttg caaaaaagtg gctaaacgtt tatcaatgtc gccaatttga atgtcttgca
     6901 taattgcaag ctcactccac gagtgcacgt tatgaaaacg ctcaaacaac atgctttcaa
     6961 cattactggc aaccgtgttg cttaaggtct gcaaatcatg gctgatttca cttttaaggg
     7021 cgattctggc ctgataaaaa cttaaaaaac tcaccagcat ggccggcaac aaccccgcca
     7081 gcaaaaaagc caacagcaac aattggcgaa tagaaagcgg tttttttagc atttaatgcg
     7141 acaatatgtc attggtttta tgtttagctg cgttaatagg tgcgacacca tgaataaacg
     7201 actcattatg atatttgcca gtgtaaccag cctgttgttg ctgtgcaagt cagcttgggc
     7261 agaagatgag atttcgtcgg atgctgttcc ggcaactttt atagatggct ttagtttagg
     7321 cggctacagt agtgcttcac tggtcatacc gcgcaatcaa tccgttgagg ccgccattga
     7381 tgaaatcagc ttgatattgc gctgggaaaa tgacggccgc tttaaatttt ttggtgagtt
     7441 agagttagag cgccccatct cttggaacga caatgagcgc attagtcaca agaatgccta
     7501 tcttgattta gagcgtttgt accttgatta caatttgtct gaaaaattaa acttgcgcac
     7561 tggccggttt ttaacacctg cggggcgctg gaacttatta cacgctgcgc cattggtgtg
     7621 gacaacgaca cgaccgctgg tgactagccg actttttcca gcatcgacca acggattgat
     7681 gctctacggc agcgtgccga tgcaaaataa agcctttgaa tacacttttt ttgtagaaac
     7741 gttaaaagac caatacaaag atgattttga aattcagtat cgcgatgtca gtggcgcgca
     7801 ctttagctta aataatgagg tcaatttggg cttaacactc atgaccttgc gccaaactga
     7861 cccgcgttcg cccgcatacc gcatgattgg gcttgatttt ctcacgcagt taggccgcct
     7921 agagttaagc ggcgaaggct actatcgttt tgccagcaat ggcaaagacg gcgggagtgg
     7981 cgcttatgtg caatctgctt atcatctggg taatgaatgg tacggactcg caaggctgga
     8041 aacatttcaa agcccgcaag aaggaagtgc cgaacgctgg ctggtgggcg cgaccaagcg
     8101 tctaaagcca aaccagttgc ttaaaatgga attcacgggc ggcagcggag agctgccaga
     8161 cgcaccgcgt ggctttattg gctcatttgc gatattgttc taatgcgtaa gtttttacag
     8221 tttttaatgc tcatcacact cgctggcctt gcccaagccg agcaggcacg gccgattgcg
     8281 gtaattatgc aaaatgatga agaggcgagc aaactggccg cagaagaatt aagcctgatt
     8341 tattggcgta aaaaacaata ttggcagggc ggcctacgca ttcacccagt caatttgcat
     8401 gccgagcacc cgctacggct caatttttca aaagtggtgc tgggcagttt gcctgccacg
     8461 caaaccaatt actggaacgg cctgtatttt cacggcacca caccgcctta cagcgtgcaa
     8521 tctgaagaag cggttatccg ctatgtggcg aataccaaag gcgccatcgg ctatgtggac
     8581 gcgtgcaaag tagatgcgcg cgtgaacgcg gttttatgga ttagcgacaa caaactcagt
     8641 gcaacaaaac cggccttaga ttgcggtagt tgaagtgctt ttttgcttgg cgaattcaag
     8701 gttaaaaata cgcttttaca gaccataatt tgcacgagga aaaataaatg ctctggaagc
     8761 ctttatttac aaggcttcca gagcatcaaa aaaggttggt gaaaaaatca ggcattttag
     8821 gcagttaatt gatgcgttaa aacaacccat tcttggcgca taatcagcgt acattttcca
     8881 tttagagatt taagttttct gattcattta acgccccagt tttataagca cgactggccg
     8941 tttggtagta ggcaatcgct tcgcgcaata aggtttcgtt catgctggcg ggcgtggact
     9001 caagtgtttt cgggtttacc cgaaaggctt cagctttttg tttagggctt aacacaatca
     9061 gcgtatcgtt tttgtaatag cccaattctt gataattgct gagaaaggcg cgcggtgcgg
     9121 tttgattttc agctggttta aacagatttt cgccaaaaaa gtgttgctcg ccctttgccc
     9181 ccaataaatg catcacggta ggtgcgatgt caatttggct tgccattctt gggtaatgcc
     9241 cagcgggtag catgtctggc gcgtaaaaaa tcagcgggat atggtattta tcaactggca
     9301 atttagtttt gcccgccgct gacgagcaat ggtcggccac aataataaat aaggtgtcgt
     9361 taaaccacgg ttttgtttta gcgttttcaa tcagtttgcc aatagcgtaa tcggtgtatt
     9421 tcacagcacc cgcgcgcttg ccttgcggca aatcgatttt accttcagga aaagtaaacg
     9481 gccgatggtt gctggtggtc atgatgtggg caaagaaagg cgttgcattt tttacgttgt
     9541 catcaatggc tttgatagca ttattaaata aactctcgtc atccacaccc caaacgtttt
     9601 cagcttgtat ggatttggca tcaaaatcgg tacggtcaat caccttgtaa tcgttgcctc
     9661 taaaatacgc gttcatgttg tcaaacatgc cataaccgcc ataaataaag aaggtgtaaa
     9721 aactttgcgc ttctaaaaac tcacctaccg tggctaaatg ctcgttgttt ggccgatgca
     9781 caatggcctg accaggaata ggcggcgtgc caagtgacag tgcatccaaa ccacgtacgg
     9841 tgcgcgtgcc tgtggcaaaa atcttatcaa acaccaagcc ttctttcgca attttatcaa
     9901 gagtaggcgt aaggttttct tggttaccgt aagtgcctaa ataatctgcc gataagcttt
     9961 caaccgatat caaaaccaca tttttggggc ggcgtttgaa cggtccgatt gctttggttt
    10021 ctatctgagg ttcagattgc agcgcttgtg ataagggttg gcgcgccaag ccattactcg
    10081 ccaaaatggc atcagcctgc gcttgcggaa tggttctgta aaatttatcg taatctagtt
    10141 cgttacggcg agctgccgcc gaaaagctaa ataagccgtt gccagacaat tcattggcat
    10201 acgcattttt gctaaattcc atttggtcca cattggtggt ttggtaacta agcacgggca
    10261 aggcaatggc cgttgccagt aaagccagtt tgtttttggt ggtttttggg cggttatcaa
    10321 agcgcacaaa acggctggca ataaacaaca gaccagcgac aagtaatgct atgccggtta
    10381 atatcaaggg tactgggtat gattcacgaa tatttccaat cacttcattg gtgtatatca
    10441 ggtagtccac cgcaatgaaa ttaaagcgtg tggtgaattc ctgccaaaaa ataaattctg
    10501 acaccgcgcc aaaaatcagc ccaaaagttg ctaaaaatgc cactaaccag cgcgctttat
    10561 tcacccaagc aagatggcgc cagcggttag gcaatagaat tgaggatata agccaaggca
    10621 ccactaaata ggccagcgcg gcaacatcaa accacaaccc tttagcaaac gcgcctagaa
    10681 tttcgccaaa ttgcattgcg cctaaaccga tattgagtgc cagcaccact cttaaaatga
    10741 gccatgtgca aagggcaaaa atcgctaaaa agccaaacaa aggcaacgta cgaaggggtt
    10801 tttgtgttga aactgtttgc ataatattgt gcgtttttct tttaaattaa tgttgaggtt
    10861 tgaattacca gaatgcttta agctttgtgt cagcttttaa tttttggcgt ccaaagccca
    10921 tacagcaata acaatgtaag ccacaccacc cagccgctcc acaacacgtg tgataaaaag
    10981 tgagcgccac gtatgatttg caccaagccc attgccaatc ccataaagat gccaagccac
    11041 agcatggcgc cggcaaagcg cgcgcgatac tgcctaaaag caaagtaaaa tgccatcagc
    11101 gcaaatccac cagaaggatg tcctgccggg aagcatccgc cagattttgt gcttgctggc
    11161 gggtttttaa acaaatcaaa aaagggtaaa tcgcctccgt aaatagttaa atcccaaggg
    11221 caagcatgca tgctgtcgtg cttgagtatg gccaccgcgc tggtagaaag caccaagccg
    11281 ataaataccc aaaacaatgc ggattgatat ggttttaatt tcactaacca atgggcagaa
    11341 atcgccagcg ccaagctcac aagcgccaca cacaccatca cccatttaat gcccgtatgt
    11401 aaccaaacat tcagaattgg atgcccgcga agcgtgaaac gctgctgctg tgcatcaaaa
    11461 aataaattgc ttaggtgaaa atccaaatca aaagttggat aaatcaacat catgaatagc
    11521 gcgaatgcta aagtccaaac accagacctt agccaaaagg cggatgtttg agcttttgca
    11581 tgattttttt ggaattgcat gtgatatttt gtgtgaataa ttgatggcgt cattaagtgt
    11641 aaagttgatt aagtctggct tattgagcaa tatctgagcc aaacagctaa cctgttgttt
    11701 tatgatgatt attgatattg cattgactat cttcatgcaa attttgcagt gagctctgca
    11761 aatttaagct ttaaatatgc aatatttgca acgaaagcgt gtttgaaaat ttgtttaatt
    11821 cacgaaaggc cagtgcttag tttgttgcaa cgaaagggtg aacgttaaaa acgcttatta
    11881 atcaataggt taaacgagag tctgaggtaa agcatattac ctgcctcaag aaaaaacgct
    11941 atccaaactt gcttggcacg cacattgcat aatatttgct atcatcatta tgaagattta
    12001 taaaatgatt acttcaccag catttagttt aagcactgca gcattttttc acgcagaggc
    12061 agcttatcaa catcgcgcat tagcagcggc aaaacgtcat cgcatcaata gcatcacggt
    12121 cgcaacttct gatgaatacg caccagaccg ccatgaactt gagcaatttg tacatgatgt
    12181 atttgcaaaa acttacggcg cagatgtgca gcaatttatg ccagagttag tcagtttgcg
    12241 tgatgaacat ggtgatttag tggcggcctt tggcttacgc aaggctacaa atagcgcgct
    12301 gtttttagag cgttatctag atgcacccgt cgaaaccgtg ttatctcaac gctttaataa
    12361 gccgattacg cgtactgcta ttacagagat tggaaattta gcagtcgcca atccgcgtca
    12421 tgcgggtatt ttaattgcac atgtgattgg gcacagttta gatatcggcg tggaatgggc
    12481 tgttgcaacc gcacatcata ccctgcaaaa tggcctgatt aaaggtggcc gcgatgtgta
    12541 cgccttgcag gcggcagata aaaacagatt aagcacagca gaacaaaaaa cttggggtac
    12601 ttactatgac aacgccccgc aggttgtggc gataaaaggc attgcgcaaa tttgagtgcc
    12661 gctaattccc ctccgttatt ggcagctatt tggcagcatg ctgaccggca acccaaccgt
    12721 gttgcgctgc aaagcttaca aaaaacagtt acttatgctg agttggcgtc agccattgcc
    12781 caagcgcaag caatattatc gctacaaacc aaacaagcaa ccattgccgt tgcgctagaa
    12841 aatcaacccg cttgggttgt actggatttg gccgcaatta ccaatggcaa tccacttgtt
    12901 ccaatacccg cctttttttc tgcggcacaa atgcggcacg cggtcagtga tgcaggtgca
    12961 accacactca ttaccgataa cccaagtaat ttacagtcca tatttgcaga aataatagat
    13021 gctacatcca cgattgaagt cgccagtata agcctcacgt tgtttatctt aaaaagtgaa
    13081 ggtaaggcat tgccacaaca aactgcaaaa attacctata cctcagggac gacgggtaac
    13141 ccaaaagggg cttgcctaag tttagcggcg atgaccaatg tggcccaagc gattcaactt
    13201 gcaaccaaat taagagcaga agatcagcat ttgtgtgtgt tgccacttgc aactttgctc
    13261 gaaaatgttg ctggtgtcta tgcggccctg ctggcagggg caaccattca cctattgcca
    13321 agcgaccaag tgggcttaat gggcagcaag ctgaatatca tgcaactgca tttggcattg
    13381 atttatacgc aggctagtac cgcaattttt attccagaaa tgttacaagc cttggtgcag
    13441 attgtagaat ctggtgcagc taagttgcca agtttgcgtt ttttggcagt gggcggggct
    13501 tctgtttcac cgcaattgtt agaccgtgcg gccaaaattg gcttacctgt gtttgaaggt
    13561 tatggcctga ctgaaagtgc ttcagtggtt tcgctcaata cgcccgaggc caataaaatg
    13621 ggcagcgctg gaaaaccact ttcacatatt gaaattaaaa ttacaggcca gcatgaggta
    13681 ttagtaaaag gggctaattt tctaggctat accaatcagc agcaacaaat agaacaaaac
    13741 gcgtttatcg acacaggtga tatcggctat ttggatgatg aaggatactt atttattaat
    13801 ggccgcaaaa aaaacgtatt tatcacctct tttgggcgta atgtttcacc agaatgggtc
    13861 gagcgtgaac ttaaagtttc tccatatatt gctcaggctg cggtatttgg cgaagcaagg
    13921 ccgtggaatg tagccgttat cgtattacga caacccatgg acaatatcgc aatcagcaag
    13981 gtgattcagg ctgtaaatgc gtacttacct gactacgcaa gcgtggcaca atggattatc
    14041 gccgatacac catttacggc tgcgaaccag caactcacca caaatggcaa aaatcgacgc
    14101 gatatgattt ggcaacacta tcaagacaaa atcaatgcat tgtattcgta aaggattaac
    14161 atgacttttt atcaaaaatt actttcagcc actgaaaaag aacgcaatca cctgttgagc
    14221 ttaccgctta ttacacaagg tgcaaaaggc caaatttcac tgccaactta tgtaggattt
    14281 ctcacgcagg cctatcatca tgtgaagcac accacgcctc ttttgatggc atgtggtggg
    14341 cgattaccag gtcattatga atggttgcgc accgcaattg gtgaatacat tgaagaagaa
    14401 atgggccatc aagaatgggt gttgaatgac attgcagcgt gtggtggcga taaagagact
    14461 gtgcggcata gtaaaacagc tgaaacaagc gcaagcacgg caacagaagt gatggtagct
    14521 tatgcttacg atatgattta tcgcgtcaat cctgttggtt tttttggtat ggtattggtg
    14581 cttgaaggca caagtacagc agtggcgact caagctggtg aaaccctcat gaatacgtta
    14641 aatttaccca aaaaagcgtt tagctacttg ttgtcgcacg gttcattaga cattagtcat
    14701 gtgtcttttt acgagagttt agtcaatcaa atcaccgacc cagaagatca ggcaatgttg
    14761 attcacagtg caaaagtgtt ttataaattg tatggtgata tatttaaaga aatagaacta
    14821 cgtttgatgt aatttaaaat ttcagaggat catcatgcaa ctcaaaggta aacgcatatt
    14881 attgacgggc gctactggcg gcgttggcat tgtattagcc cagcaactgg cgctaaaagg
    14941 cgcaaggttg gcgttagtgg ggcgtgactt aactaagcta aacgcattaa aacaaagtct
    15001 tgttgctcaa aatggtgagc atgccgtgat tactgcaaat tttgatacgg caggcacaac
    15061 tcagccattg gttgatgagg ctgtaaaaaa actagaaagt attgatattc ttatcaacaa
    15121 cgccggaata ttagatttca ttcaacttga agaccaacca gaacaacgta ttgcacaaat
    15181 ggtgcaaacc aatgtcacta cgctaattca attaactcgc gccttgttac caagcttcaa
    15241 ggctaaaaat caaggtcact ttgtgatgat agggtctatt tttggctcgc ttggttttcc
    15301 acattatgca acttactgcg ctactaagtt tgctgtgcat ggtttttcac aagcattacg
    15361 tcgtgagttg gttaacacca acattaaagt tacttacatc gcaccacgtg gcatcaaaac
    15421 aaccatgaac gacgccaaca ctacggccat gatggaacaa tcgggcaaca aaatggacac
    15481 gccagagatt gtcgcgagca tcatcatcaa agcgatagaa aatgaaaaac aagaagtatt
    15541 tataggccag ccgcaatctt tttttgcttg gctaaatggc gtgctgccga tggcggttaa
    15601 ttttggactt aaaaaacaaa cggcacttgc tgtgccgttt ttgcgtaaaa aataagctcg
    15661 atttatttgt tttatagata aagcaccgct ggaaaaacag tcagtgcaaa tacaaaaacg
    15721 agccaatcca tattccaacg ttctaagtga ccagtaaagt gcagaactag cacagcaatc
    15781 gccaaaagat aataaattgc aatcagtaac ataaaaagcc caaatcttgt gaatatcaga
    15841 atcaatcagt gtataacggc accagctaag aataccttaa ttttaaattt ttttgtactt
    15901 ttttgcacag gcatttcttg cctaaagcgt gtttttcgcg taaatttagt ctattgctag
    15961 gggtctgttt caaatttgat cattcaaaat taaaacagtg agagataccc tttgaacctg
    16021 atgcgggtga tgccgccgta gggaagcgtg tcttagcttc cctattttat aaacagactc
    16081 ggaagcgctt gttggcttcc ttgcaatgaa tcaatgaata ggaatgcaca ccgtgaatgc
    16141 caatcccaag tttttaaaca ctactgcaga agttgatcct gcaaccactc agccatttgc
    16201 aaagtctcgc aaagtctatg tagagggtag ccgccctgat attcaagtgc cgtttcgtga
    16261 aattagcttg agtgatacac cctctgcctt tggtgctgat aaaaatccac ccgtgatggt
    16321 gtacgacact tcaggcccat atactgaccc aaccgcaaaa attgatattc gtagtggctt
    16381 gccagcgcta cgtgccaagt ggattgagga acgtaacgat actgagcaac tcgatggccc
    16441 cacttcaact tttggccagc agcgcaaagt agaccctgaa cttgcagaaa tgcgctttaa
    16501 tttaacgcgc ttgccacgcc gtgcaaaggc gggcatgaat gtgagccaaa tgcattatgc
    16561 gcgtaaaggc attattacgc ctgaaatgga atacattgcc attcgcgaga atcatcgccg
    16621 tgaaaatatg agtgaactgt tgcaaacaca gcacaaaggc cacgactttg gtgcaagcat
    16681 tcctaaaatg attacgccag aatttgtgcg tgatgaagta tcgcgtggtc gcgccattat
    16741 tccaatgaat atcaaccatc cagaaattga gccgatgatt attggccgta atttcttggt
    16801 aaaaatcaac gcgaatatcg gcaactcggc attgggttcg tctattagtg aagaagttga
    16861 gaaaatggta tggagtacgc gctggggcgg cgacactgtg atggatttgt ctacaggtaa
    16921 aaatattcac gaaacgcgcg agtggattat cagaaattca cctgtaccga ttggcactgt
    16981 gccgatttat caggcacttg aaaaagtgga cggtaaagct gaagacctca cttgggaaat
    17041 ctttcgtgat accttaattg agcaggcaga acaaggcgtg gattatttta cgattcatgc
    17101 cggtgtacgc ttggcttaca ttccaatgac tgcaaaacgc atgacgggta tcgtgtcacg
    17161 cggcggttca attatggcga aatggtgttt ggcacatcat aaagaatcat tcctttatga
    17221 gcattttgaa gatatttgcc aaatcatgaa gcaatacgat gtttcattta gtctaggcga
    17281 tggcttacgc ccaggttcaa tttacgatgc caacgatgaa gcacaatttg cagaattgaa
    17341 aacactaggc gagctcacac aaatcgcgtg gaagcatgac gtgcaatgta tgattgaagg
    17401 cccaggccat gtgccgatgc acctcattaa agaaaatatg gacttgcagc ttgagcattg
    17461 cggcgaagcg cctttttaca ccttaggccc tttgacgaca gatattgcac cgggttatga
    17521 ccacattaca tctggtatcg gtgccgccat gataggctgg tatggttgtg ccatgctatg
    17581 ttacgtcaca ccaaaagagc atttaggttt gccggataaa gaagatgtgc gcgtaggcat
    17641 tattacttat aaaattgcag cgcacgcggc agatttggct aaaggtcatc cgggcgcgca
    17701 aattcgtgat aatgcattga gcaaagcgcg ctttgagttc cgatgggaag accaatttaa
    17761 tctcgggttg gacccagaaa aagctaaaga atttcatgat gaaacgctcc cacaagaagg
    17821 tgcaaaacag gcacatttct gctcaatgtg tggcccacat ttctgctcaa tgaaaatatc
    17881 acaagatgtg cgtgattatg cggccgaaaa aggcgtttct gagcaagaag cgttgacgaa
    17941 aggcatgcaa gaaaaagcca ttgagtttgt gaaaaaaggc agtgaggttt atcagaaggt
    18001 gtaatacttg taaccgttag gcgtaaaaaa agcaggcgtt aagcctgctt tttttacgca
    18061 ctaggcaata tgcttagcta gtcgtaataa gttacaggta taaccatact cgttgtcata
    18121 ccagcctatg acttttaaaa aggttgaatc gagcatgatg cctgcgtctg catcgaacac
    18181 tgaagccgct ggtgcagtac gaaaatcagt ggaaacgact ttttcttctg tgtaggctaa
    18241 tacgttttta agttcgcctt gcgccgccgt tttcattgcc tcgcacacct gcttgtacgt
    18301 ggcttctttg ttgagctcta cggttaagtc taccactgaa acgtccgctg aaggcacccg
    18361 cagcgccatg cctgtgattt ttttgtccaa tgatggaatc aacacgccaa tcgctttggc
    18421 ggcccctgta cttgaaggaa taatattttc aaacacaccg cggccaccgc gccagtcttt
    18481 tttagaagtg ccatcgacag tgagttgaga agccgttgcc gcatgcactg tcgtcatcag
    18541 gccacgtttt acgctaaagt tatcgtttaa cactttaacc aatggcgcta gcccgttggt
    18601 agtacatgaa gctgctgaaa caatcgcttg gcctttgtaa tcctgatgat ttacgccata
    18661 gacaaacatt ggggtatcgt ctttacctgg tgcggattgc actacttttt tagcgccacg
    18721 ctccaagtga acttcgcagc tttcttgagt taaaaatttc cccgtcgatt ccactaacac
    18781 atcaacacca ctcgtatccc agtcaatata ggccgggtca cgttctgaag tgacatgaat
    18841 ttttttacca ttaacgacca aatgtccgtt ctcggtacgc acatccgcat taaaacgacc
    18901 atgtaccgag tcatatttca gcatatacac catgtaatca agttcttgcg tgccgttgac
    18961 agccacaatt tcaatgtcgc taaattcggc ttcttg
//
LOCUS       DAOQSI010000019        18827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_2059_length_18827_cov_5.277541, whole genome shotgun sequence.
ACCESSION   DAOQSI010000019 DAOQSI010000000
VERSION     DAOQSI010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 18827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18827
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2059_length_18827_cov_5.277541"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PKL42_02495"
     CDS             complement(<1..314)
                     /locus_tag="PKL42_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65969.1"
                     /translation="MSRNPSDTAAAYNQAAVLAREVGARMAERLDYMKISPQRMADIG
                     CATGDGIRALQARYPKTHALAIDVSLPMLHGVRAHNSRLTRLLNRLFNHGPQLVNADA
                     RAL"
     gene            complement(323..1309)
                     /gene="bioB"
                     /locus_tag="PKL42_02500"
     CDS             complement(323..1309)
                     /gene="bioB"
                     /locus_tag="PKL42_02500"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HNU65970.1"
                     /translation="MVINTDSLKHHSKQCSPTVNRWRVEEIAALYELPFNDLMHQAQT
                     VHRENFDPNGVQVSTLLSIKTGGCSEDCGYCPQAARYHTDVENEPMMAIDEVLAAAKE
                     AKDNGASRFCMGAAWRSPKQRDLEPVLKMIAEVKAMGLETCATLGMLKDGQAAQLKEA
                     GLDYYNHNLDTSPEFYGQVITTHTQADRFDTLAQVRSAGIKVCCGGIVGMGETRGARA
                     GLLAELANMPTPPESVPINQLVPMPGTPMGHVAPLDPFEFVRTIAVARILMPSSMVRL
                     SAGREEMSDEMQALCFLAGANSIFYGDKLLTAGNPQADRDEALFNRLGLISV"
     gene            1391..2110
                     /locus_tag="PKL42_02505"
     CDS             1391..2110
                     /locus_tag="PKL42_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNU65971.1"
                     /translation="MKTTQIINNWLKFNQRLFSGQCLLCTAPSQQDADLCSACFQALP
                     WHNVSQCLQCGLPSNHPICGHCLHEPPDFDATFAALRYEFPLDAVMQHYKYGSALQTA
                     HLFAHLMTTQLAKQQDKRAIDLIMPMPLHPQRLKERGFNQSLEIARILSKSTHIPLDF
                     QSAIRTIYTPPQASLALKERAKNIKGAFAVHADLSNQRIAIVDDVMTTGASLNELAKM
                     LKKAGAAHVECWVVARTLPPT"
     gene            2259..2723
                     /gene="trmL"
                     /locus_tag="PKL42_02510"
     CDS             2259..2723
                     /gene="trmL"
                     /locus_tag="PKL42_02510"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HNU65972.1"
                     /translation="MFHIVLFEPEIPPNTGNIIRLCANTGAQLHLVKPLGFSLDDKQL
                     KRAGLDYHEYANLQVHENWPACKTALAGKRMFAITTKGSSHYANTQFKDEDVFVFGPE
                     TRGLPEDIRNEFLAEHRLRLPMLPDSRSLNLSNSAAVLLYEAWRQIGFDGGV"
     gene            complement(2724..3239)
                     /locus_tag="PKL42_02515"
     CDS             complement(2724..3239)
                     /locus_tag="PKL42_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNU65973.1"
                     /translation="MPTIFSHVAVPIATSLGIGKIVSRRLLAFSAFCAVLPDFDTIGF
                     KLGISYASQWGHRGFTHSITFAVAVALLAMCFSRPLKSRAWVVFLLTFVAVVSHPLLD
                     MLTSGGLGVALYWPYSNERLFFEVRPIAVSPIRIANFFTERGWRVIKSELLWVWLPSV
                     ILLFTLRGMRK"
     gene            complement(3349..4353)
                     /locus_tag="PKL42_02520"
     CDS             complement(3349..4353)
                     /locus_tag="PKL42_02520"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNU65974.1"
                     /translation="MRKIAVLGAGAWGTALAMNISERHLVNLWARNAGHVSGMRKARA
                     NPLYLGDFQFNDQLQVQDDLATAISGADLILSVVPAAGFRGILEDLKALGCNLPIIWA
                     HKGLEPQSAKLPYEVALEELGDPKQTGQHWGALSGPSFAAELVRGLPTAVTLAATDEA
                     FSNEAALLIHGANLRVYHSTDVIGVSVGGALKNVMAIAAGISDGMGFGNNARAALITR
                     GLAEITRFGVALGASTETFMGLAGVGDLILTCTGQYSRNREVGLQLASGKSLADILQG
                     LGHVAEGVNTAREVMRRAETIGVDMPITYEVNQALSSGKSAKDAVTDLLGREQKPEAI
                     "
     gene            complement(4384..4839)
                     /locus_tag="PKL42_02525"
     CDS             complement(4384..4839)
                     /locus_tag="PKL42_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNU65975.1"
                     /translation="MWQIKRKVTIVGLLVLMPLTAFALDFRSIAQPKAVLYDAPSAAA
                     TKVLLLNQHYPVEVVVNLGDWLKVRDAQGSLNWVEAKYLSNKRTVLITQNNAEIRQSA
                     DARSTLLATVEKDVLLEVADAKPSRGWLRVKHRDGLTGYMLLSSTWGSQ"
     gene            complement(4859..5332)
                     /gene="secB"
                     /locus_tag="PKL42_02530"
     CDS             complement(4859..5332)
                     /gene="secB"
                     /locus_tag="PKL42_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HNU65976.1"
                     /translation="MAQEDNALAEQQAQPGFSIEKIYVKDASLEIPNAPQIFTDRTQP
                     QVSIELSNAAQALEDGVFEVALKVTVTSKIEDKTVFLVEVTQAGIFQIRNVPEENLDM
                     IIGITCPNILFPYVREAVSDLVVRAGFLPVLLNPINFEALYAQQKQQQAEAAAKH"
     gene            complement(5453..5707)
                     /gene="grxC"
                     /locus_tag="PKL42_02535"
     CDS             complement(5453..5707)
                     /gene="grxC"
                     /locus_tag="PKL42_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HNU65977.1"
                     /translation="MPVITMYSTAVCPYCINAERLLLSKGIVEINKIRIDLQPEKRDE
                     MMQKTGRRTVPQIYIGERHIGGFDDLRALDLAGGLDPLLA"
     gene            complement(5707..6171)
                     /locus_tag="PKL42_02540"
     CDS             complement(5707..6171)
                     /locus_tag="PKL42_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNU65978.1"
                     /translation="MEFIKNNILLIGLVLGSGIMLLLPSIKKSANGVPNLSPAEAVTL
                     INRSNAVVLDVRDAAEFATGHIAEAKHIALDDLPAKLNDLKKYQNKAVLVNCQRGMRS
                     AKACEMLRKAEFTQVHNLQGGLDAWVNAKLPVVTSQAPAKKTTSKPAEKAES"
     gene            6360..7931
                     /gene="gpmI"
                     /locus_tag="PKL42_02545"
     CDS             6360..7931
                     /gene="gpmI"
                     /locus_tag="PKL42_02545"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNU65979.1"
                     /translation="MLPAANLTPVCLLILDGFGYREESANNAIALARKPNIDALWNNM
                     PHTLINASEHYVGLPDGQMGNSEVGHLNIGAGRVVFQDFERINNSIKTGEFFEHQALK
                     SALLNIKNNNKALHILGLLSDGGVHSHHEHIYAMLEMAKKLGLTNVYVHVFLDGRDTP
                     PVSAAPYIAALEAKIQALGVGKIISVSGRFYSMDRDKRWPRVEAAYALITEGIGEFTF
                     ATANEALQAAYARGENDEFVKCTAIRKPNEAATHLEDGDLVVHMNFRSDRARQLTHSL
                     LSEEFDGFHRRHVPALSGYFTLTMHDVKETKATAIFPPFAVKNTFGEYLSSLGLTQLR
                     IAETEKYPHVTFFFNGGEEKVFTGEDRILVPSPQVATYDMQPEMSAFELTKKLEEAIL
                     SRKYNAIICNYANGDMVGHTGSLQAAIEAIETLDTCVGRIVNAMQSIDGEVIITADHG
                     NAETMFDVENNQPHTQHTTNKVPLIYVGREATLADDGALSDLAPTLLHLMGVAQPSEM
                     TGKNLVNFKSSNSPK"
     gene            8048..9283
                     /locus_tag="PKL42_02550"
     CDS             8048..9283
                     /locus_tag="PKL42_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNU65980.1"
                     /translation="MLLLTPAAHAAKKIDESKQDLNEVKARIESLKKELDQSQEAHKD
                     AADALKESEVAISIANKKLYEINNKQQVNKKELSHLEKQSYATNQALSHQQKRLSSQL
                     YQQYTRGEQSYAQIILQNEQPSAIARDVQYFSYVAKARAELIQDMRRNLEKINQLNDE
                     TASKLKEVAELQQKQLQEKKALENQKQAKSKVVSSLSQQIAAQRNEIQKLKRDEKALS
                     DLVERLAKIVPPKKKIVKKTTTPKPGKQDSDSTKPLPTKQTEEEVIENNDEPSADDGL
                     AGANFAALKGKLRLPVRGDVTNRFGSTRPDSGISWKGLFIKATEGAEVKSIATGRVVF
                     ADWLRGFGNLIIVDHGSGYMSLYGNNQAVLKQVGDTVQTGDAIASVGNSGGNETNGLY
                     YELRRQSKPFDPLSWSKLN"
     gene            9587..9919
                     /locus_tag="PKL42_02555"
     CDS             9587..9919
                     /locus_tag="PKL42_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNU65981.1"
                     /translation="METKSAVKLLSSIAQEARLDIFRLLVQAGSDGLAAGVIGEKLQI
                     PPSTLSFHLKELSISGLVKSRQVSRFIYYAANYEAMNGLLTYLTENCCAGNAACCGNT
                     TCDDSIKN"
     gene            9938..10417
                     /locus_tag="PKL42_02560"
     CDS             9938..10417
                     /locus_tag="PKL42_02560"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNU65982.1"
                     /translation="MNILFLCTGNSCRSILGEATFNHLAPAGWRAMSAGSKPAGYVHP
                     RSVALLAREGISTEGYYSKSWESLPTTPDIVISVCGNAANETCPAYLGPVLRSHWGVE
                     DPAHATGTDEEIDAAFMKAYRILRARIETFLALPLETLKHDKPALKLAMDKIATIVV"
     gene            10430..10789
                     /gene="arsD"
                     /locus_tag="PKL42_02565"
     CDS             10430..10789
                     /gene="arsD"
                     /locus_tag="PKL42_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter metallochaperone
                     ArsD"
                     /protein_id="HNU65983.1"
                     /translation="MKTIQVFDPAMCCSSGVCGVEVDQQLVSFSADVDWAKQNGAKIE
                     RFNLAQQPMDFANNKVVRDFLERSGQEALPLILVDGEFALAGRYPNRTELSRWAGIVA
                     ITEIAPVGGCCSGGKCC"
     gene            10809..12572
                     /gene="arsA"
                     /locus_tag="PKL42_02570"
     CDS             10809..12572
                     /gene="arsA"
                     /locus_tag="PKL42_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical pump-driving ATPase"
                     /protein_id="HNU65984.1"
                     /translation="MKFLDQAPRFLFFTGKGGVGKTSIACATAIQLAEAGKRVLLVST
                     DPASNVGQVFGITIGNNITAVPAVQNLSALEIDPQAAAQLYRDRIVGPVRGILPDAVV
                     KGIEEQLSGACTTEIAAFDEFTSLLIDSPLTTGYDHIIFDTAPTGHTIRLLQLPGAWS
                     GFLEEGKGDASCLGPLAGLEKQREQYKAAVDALADEKRTRLILVARAQLSTLREVART
                     HEELAGIGLKQQYLVVNGILPKAEAENDALAAAIYKREQAALNAIPEVLKSLPQDNVM
                     LKPFDLVGLEALRQLLVDTPHHAQVVQISPKVLDNPSLSKLVDEIAEDGHGLVMTMGK
                     GGVGKTTIAAAIAVELARRGLPVLLTTSDPAAHLSDTLNGALNHLTVSRIDPHEETER
                     YRQHVLETKGSKLDAQGRALLEEDLRSPCTEEIAVFQAFSRIIREAGKKFVVMDTAPT
                     GHTLLLLDATGAYHREVARQMNKSHLHYTTPMMQLQDPKQTKILIVTLAEKTPVLEAA
                     NLQADLRRAGIEPWAWVINNSVAATSTHSPLLHQRALNELVEINSVAQQHAKRYAVLP
                     MLKDEPRGLTRLLELAGNKHL"
     gene            12670..13104
                     /locus_tag="PKL42_02575"
     CDS             12670..13104
                     /locus_tag="PKL42_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HNU65985.1"
                     /translation="MNRREALLSGAAIAAAAVVGRAQAAEMQHHHHAAMANTALVSAA
                     ADCIQKGQVCLNHCLYLLGQGDKEMAACAKSVNEMLALCGALQSLSNQESATLPRLAA
                     IAMDACNQCEEECKKHADKHDVCKACGESCNACAKECKKIAA"
     gene            complement(13194..13688)
                     /locus_tag="PKL42_02580"
     CDS             complement(13194..13688)
                     /locus_tag="PKL42_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNU65986.1"
                     /translation="MSALITSPVTELLLQQGIAFSVIEIPLTEDKKPIRNLEALLTQQ
                     GINPQSVVRSVLFKAGSEQFVLLAVAGGGRADWAVLRQHLNARKCRMAEFDEVQTATG
                     YIVGAVPSIALPSEIKILVDNSVKDYPIVVIGSGVLGYALSLNSQDLFTLLADAEQGR
                     FTSA"
     gene            13779..14789
                     /locus_tag="PKL42_02585"
     CDS             13779..14789
                     /locus_tag="PKL42_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNU65987.1"
                     /translation="MKNSAIIVNTGGGYNNVTFGHREATAPRAGEIKVRLHANSLNYH
                     DFAVVSGMWGPTEPRIPMADGAGVVLEVGEGVTEFSVGDHVVSTFFPTWLAGEPLVEG
                     FATTPGDGVDGYAREVVTAMANAFTLAPKGWSHIEASTLTTAALTAWRALMSDDALKP
                     GDTVLIQGTGGVSIFALQFAKMAGATVIATSSNDEKLERLKSFGADHLINYKKTPQWG
                     NVVREITNHQGVDHVIEVGGPATLEQSMTAARIGGHISVIGILTGLGGEISIVSALIK
                     QLRLQGVLVGNRTQQQEMIKAINANNMRPVIDKVFALENIVGAFRYQETNQHFGKICL
                     EM"
     gene            complement(14847..>15590)
                     /locus_tag="PKL42_02590"
     CDS             complement(14847..>15590)
                     /locus_tag="PKL42_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65988.1"
                     /translation="SLYTACDSAQGALKLHLPAIEYCAEISLGANRQKSFDAIKTHLD
                     HFFQNNALSQIDYSVKDTWRVTWQVQSVSVELYCFYREMPDGWDNVAWLRIRYAPNLD
                     RFYRTDYQRNLVLNAAIQYATFDFAIDLNANYRQVENAIYTPDCFKPMITEAQPLLIW
                     HDTQQNVIGFADATLALIFNVNEVEKLTLAVQNFRGSEGRNGLEVHVQRETTHLGGVS
                     NVAKFKKNIKKITKLIDKEVSTYTYDEHY"
     assembly_gap    15591..15690
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15691..16153)
                     /locus_tag="PKL42_02595"
     CDS             complement(<15691..16153)
                     /locus_tag="PKL42_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65989.1"
                     /translation="MRSYSAQALSVCTLAGNFAALERIVTQLPNFDVERYTAFRNAKV
                     DMAEAVLWQKVHTADFAISPTDVADNGKSALDLLQQGLWLEHLNTLIAWGTYEDLQNN
                     KHIKTQRKDFPNPSFHATEYCITKPTIFNGLKLHSLYTACDSAQGALKLHLP"
     gene            16172..16354
                     /locus_tag="PKL42_02600"
     CDS             16172..16354
                     /locus_tag="PKL42_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65990.1"
                     /translation="MRVKIIKPINRDVIDVIQVNLAVIMMMANEAKVMAVNHQTLTIK
                     WVLVRRFFWYENFAYE"
     gene            complement(16556..17974)
                     /gene="cysS"
                     /locus_tag="PKL42_02605"
     CDS             complement(16556..17974)
                     /gene="cysS"
                     /locus_tag="PKL42_02605"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNU65991.1"
                     /translation="MLKIYNTLAREKQVFTPIQAGKVSMYVCGMTVYDFCHLGHARVM
                     VVFDMVSRWLRTSDYEVTYVRNITDIDDKIIKRANENNETISQLTQRFIDAMDEDSAR
                     LGVIRPTLEPKATEYVSGMVEMIQTLINKGFAYTAANGDVFYAVNKFDGYGKLSGKSL
                     EDLRAGERVEVDSFKQDPMDFVLWKAAKPNEPSWDSPFGGPNNGKGRPGWHIECSVMS
                     SHLLGAHFDIHGGGQDLQFPHHENEIAQSEAAHGGVWNGEATHSHDGQPCQMVNYWMH
                     NGFVRVDDEKMSKSLGNFFTIRTVLEKYDAEVVRFFILRAHYRSPLNYSDQHLDDAKL
                     ALTRLYTALRGHDVASNTIDWQHPQAVRFKAAMDDDFNTPEAMAVLFDLANEVNKSKS
                     AETAGLLKSLAGVLGLLQRSPDAFLQGEATQTLNIDALIQARAEAKKAKNFAEADTIR
                     KQLLEAGVVLEDSPQGTTWRRI"
     gene            18128..18700
                     /locus_tag="PKL42_02610"
     CDS             18128..18700
                     /locus_tag="PKL42_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNU65992.1"
                     /translation="MRHLFALLMLVFFSHSALAADTKTNPQVTFETTHGNIVLELYPQ
                     KAPKTVANFLQYAESGFYKDTIFHRVINGFMIQGGGFTADMQEKPTNAPIKNEAANGL
                     TNDKGTIAMARTSDPDSATAQFFINLVNNHALNYRGDSPEEIGYCVFGRVLTGMDVVR
                     EIAIVNTGNAGPHGNVPKSPIIIKQVKIVK"
     gene            18712..>18827
                     /locus_tag="PKL42_02615"
     CDS             18712..>18827
                     /locus_tag="PKL42_02615"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNU65993.1"
                     /translation="MVKLQTNFGDITIELNAEKAPITVANFLQYVDNGFYDGT"
BASE COUNT         5282 a         4448 c         4113 g         4884 t
ORIGIN      
        1 agcgcgcgcg catcagcatt gacgagttgc gggccgtgat tgaataggcg attgagtaag
       61 cgcgtgaggc gtgaattgtg tgcacgtacc ccgtgcaaca ttggtagcga aacatcaatt
      121 gccagtgcgt gcgtttttgg gtaacgcgct tgtaaagccc ggatgccatc gcctgtcgca
      181 cagccgatat cagccatgcg ttgcggcgaa attttcatgt aatcgaggcg ctctgccatg
      241 cgtgcaccca cttcgcgggc gagcacggcc gcttggttgt aagcggctgc tgtatcagat
      301 ggattgcggc tcacaccgca aatcacactg aaatcagacc caggcggtta aacagcgctt
      361 cgtcgcgatc ggcttgcggg ttgcctgcag tgagtagctt gtcgccgtag aaaattgaat
      421 ttgcaccggc cagaaaacac aaggcctgca tctcgtcgct catttcttca cggcccgccg
      481 agagtcgcac catggagctt ggcatcagaa tgcgcgctac cgcaatcgtg cgcacgaatt
      541 caaacgggtc gagcggtgcg acatgcccca ttggtgtgcc gggcatcggc actaactgat
      601 tgatgggtac cgattccggc ggcgtgggca tgttggccag ctcagccagc aaaccggcgc
      661 gtgcgccgcg tgtctcgccc atgccgacaa taccgccgca acataccttg atgcctgcgc
      721 tgcgcacttg tgccagcgtg tcaaaacgat ccgcctgcgt gtgcgtggta atcacctggc
      781 cataaaattc gggcgaggta tcgagattgt ggttgtaata atccagcccg gcttctttga
      841 gttgcgccgc ttggccatct ttaagcatgc ctaacgttgc gcatgtttct aagcccatcg
      901 ctttaacttc agcaatcatt ttaagcaccg gctctaaatc gcgctgtttc gggctacgcc
      961 atgccgcgcc catacaaaag cggcttgcgc cattgtcttt ggcttctttg gcggctgcca
     1021 gcacttcatc aatcgccatc atcggctcat tttcaacatc ggtatgataa cgcgcagcct
     1081 gcggacaata tccgcaatcc tcactgcaac cacctgtttt tattgataac aacgtactca
     1141 cttgcacacc gtttggatca aaattttcac ggtgcaccgt ttgcgcctga tgcatcaaat
     1201 cgttaaatgg caattcatac aacgctgcaa tttcttcaac acgccagcga ttcactgttg
     1261 gtgaacattg tttgctatga tgttttaaac tatcggtgtt aatcaccatt tctgttgttg
     1321 attgcatcgt gaaaaccttt taattggcgc attaagcgta ttgatgaata accgaattat
     1381 aagctgtaaa gtgaaaacca cacaaatcat taacaactgg ttaaaattta atcaacggtt
     1441 attttctggc caatgtttgc tatgcaccgc acccagccaa caagatgctg acttatgctc
     1501 ggcctgtttt caggcgcttc catggcacaa tgtatcgcaa tgtctgcaat gcggtttacc
     1561 gtccaaccat cccatttgtg gccactgcct gcatgaaccg ccagattttg atgccacatt
     1621 tgccgccttg cgttatgaat ttccgctaga cgcagtgatg caacattata aatacggcag
     1681 cgcactacaa accgcgcacc ttttcgcgca cctcatgacg acacaactgg cgaaacagca
     1741 agacaaacgc gcaattgatt taatcatgcc catgccgttg cacccgcagc gattaaaaga
     1801 acgtggtttt aatcaatcgc tagaaatcgc acgcatactc tccaaatcca cgcacattcc
     1861 gcttgatttc caaagcgcca ttcgcaccat atacacgcca ccacaagcaa gtcttgctct
     1921 caaagaacgg gctaaaaaca tcaaaggcgc gtttgctgtt catgccgatt taagtaatca
     1981 gcgcattgcc attgtggatg atgtgatgac aacaggtgcc agcctaaatg agcttgccaa
     2041 aatgcttaaa aaagctggcg cggcacacgt agaatgctgg gtggtggcgc gcactttacc
     2101 gcctacttag cccaagaaaa acatatcaaa aatagcaaaa aatgcacaca accttttttg
     2161 atgctctgta agccttataa ataaaggctc gcagaggcat tattttttct cgtacaaaaa
     2221 accttaatca ttgaaagctt ctaagttaaa atactgctat gtttcatatc gtcctttttg
     2281 agccagaaat tccacccaac acgggcaata ttattcggct atgtgccaac accggtgcgc
     2341 aactgcattt ggtaaaaccg ctagggttta gcttagacga caagcaactt aagcgcgcgg
     2401 ggctggacta tcacgaatac gccaacctgc aagtgcatga aaattggcca gcttgtaaaa
     2461 ccgccttagc gggcaaacgt atgttcgcaa ttaccaccaa aggcagcagc cattacgcaa
     2521 atacgcagtt taaagatgaa gacgtgtttg tgtttggccc tgaaacgcgc ggtttgcctg
     2581 aggatattcg caatgaattt ttagcagaac atcggctgcg cttgccgatg ttgccagata
     2641 gccgcagttt gaatttatct aacagcgcgg cagtattgct atatgaggct tggcggcaga
     2701 ttggctttga tggcggcgtg tagttattta cgcatgccac gcaaagtaaa tagcaaaatt
     2761 accgatggca accacaccca aagcaattct gatttaatca cccgccaacc gcgctcggta
     2821 aaaaaattgg caatcctaat cggtgaaacc gcaatcggcc gaacctcaaa aaacaagcgc
     2881 tcgtttgaat atggccaata aagggctacg cccaagccgc cgctagtgag catatcaagc
     2941 aatgggtgcg acaccacagc aacaaaagtc aataaaaaaa ctacccaagc acggctcttt
     3001 aatgggcgcg aaaaacacat ggctaatagt gcaacagcta ccgcaaaggt gatggaatgt
     3061 gtaaaaccac gatgccccca ttgcgaggca taagaaatac ctaacttaaa accgatggtg
     3121 tcaaaatctg gcaaaacggc gcaaaaagct gaaaaagcca gcaaacggcg gctaacaatt
     3181 ttgccaatgc ctaaactggt ggcaatgggt acggcaacgt gcgaaaagat ggtaggcatt
     3241 ggcgttttag ctaactgatt gatgacgtgc ctagcttaaa tcgcttgtgt gaagtgaggg
     3301 tgaagttaga ctggtgctgt tttggactga ccgctcaaaa cggacgcact agatggcctc
     3361 tggtttttgt tcacgcccaa gcaaatcagt caccgcatct tttgcacttt ttccgctaga
     3421 taaagcctga ttgacttcat aggtaatcgg catatccaca ccaatggttt cagcgcggcg
     3481 catcacttca cgcgccgtat tcacgccttc tgctacatgg cctagccctt gcagaatgtc
     3541 ggcaagcgat ttgccgctgg cgagctgcaa gcccacttcg cggttgcgcg aatattgccc
     3601 agtacaggtt aaaattaaat cgcccacgcc agcgagcccc atgaatgttt cagttgaagc
     3661 acccaaggcc acaccaaaac gggtaatttc agccaatcca cgggtaatca aagcagcacg
     3721 cgcattattg ccaaaaccca tgccatcact aatacctgcg gcaattgcca tcacattctt
     3781 taacgcgcca cccacagaaa caccaatcac atcggtgctg tgatacaccc gcagatttgc
     3841 gccatgtatc aacaaggccg cttcatttga aaacgcttca tctgttgccg ctaaggtaac
     3901 cgccgttggc aaaccgcgca cgagttctgc cgcaaaactt gggcctgaca acgcgcccca
     3961 atgctgtcct gtttgttttg ggtcgcctaa ttcttctagc gccacttcgt agggtagttt
     4021 tgctgattgc ggttctaggc ctttatgtgc ccaaataatt ggcaggttgc aaccaagcgc
     4081 tttgaggtct tccaatatac cgcgaaatcc ggcggcaggc actacagata aaattaaatc
     4141 agcgcccgaa atcgccgtgg ccaaatcatc ttgcacctgc aattggtcgt taaattgaaa
     4201 gtcgcctaaa tacaatggat tagcgcgcgc tttacgcata cctgaaacat gcccagcgtt
     4261 acgcgcccac aaattcacca aatgacgctc gctaatattc atcgccagcg ccgtacccca
     4321 cgcacccgcg cctaatactg cgattttacg cacccgttga gtcgccaaga tttcgccaac
     4381 catttattga gacccccagg ttgaagaaag caacatatag cccgtcaggc catcacggtg
     4441 tttcactctt agccagccac gactcggctt tgcgtcggcc acctcaagca acacgtcttt
     4501 ttcaacggta gccaataatg ttgatcttgc atcagcgctt tggcgaattt ccgcattatt
     4561 ttgcgtaatt aacactgtgc gcttattaga taaatattta gcctcaaccc aatttaggct
     4621 accctgggca tcacgcactt tcagccaatc acctaaattc accaccactt ctactgggta
     4681 atgttgattc agcaacaata cttttgtcgc agcggcagag ggcgcgtcat acaagacggc
     4741 tttcggttgc gcaatcgaac gaaagtctag cgcaaacgcc gttaacggca tcagcactaa
     4801 caaaccaaca atggttactt tgcgtttaat ttgccacaac cttttaatgt aagcaaaatt
     4861 aatgtttagc cgcggcttca gcttgttgct gtttttgctg cgcgtataaa gcctcaaaat
     4921 tgatagggtt gagcaataca ggcaagaaac ctgcacgcac gactaaatca gacactgctt
     4981 cacgcacgta ggggaacaag atattcgggc atgtgatgcc gataatcatg tctaagtttt
     5041 cttcaggcac attacggatt tggaaaatac ccgcttgtgt aacttcaacc aaaaatacgg
     5101 ttttatcttc gatttttgaa gtgaccgtta ctttcaaagc cacttcaaat acaccatctt
     5161 caagcgcttg tgcagcgtta ctgagttcga tgctcacttg tggttgcgta cggtctgtga
     5221 agatttgcgg tgcattagga atttctaatg aagcatcttt gacataaatt ttttcaatgc
     5281 taaagcctgg ttgcgcttgt tgttctgcta gtgcgttatc ttcttgtgcc ataatttttc
     5341 tactttaaat gttgatgagt ttgccaaaca aactagccaa acctcaaggt taataaaaat
     5401 actgctgttt ttagggggtt attttatcag gttttaacgg actctaaacg atttaggcta
     5461 acagcggatc tagcccacct gctaaatcta gtgcgcgcaa atcatcaaaa ccgccaatat
     5521 ggcgttcacc aatatagatt tgcggcacgg tgcgccgacc tgttttctgc atcatttcat
     5581 cgcgcttttc cggttgcaaa tcaatacgaa ttttattgat ttctacgatg cctttgctaa
     5641 gcaacaagcg ttccgcattg atgcaataag ggcacacagc ggtggaatac atggtaatga
     5701 caggcattag gattcggcct tctcagcagg tttacttgtt gttttttttg ctggtgcttg
     5761 ggacgttacc acgggtaact ttgcatttac ccaagcatct aatccacctt gcaaattatg
     5821 cacctgcgta aattctgctt tacgcaacat ctcacacgcc ttggcagaac gcataccgcg
     5881 ctgacaattg actaacactg ctttattttg atatttcttc aaatcattta gtttagccgg
     5941 taaatcatcc aatgcaatat gcttggcttc tgcaatatgg ccagttgcaa attccgcggc
     6001 atcgcgcaca tccaacacca cagcatttga gcggttaatc aaagtcaccg cttccgcagg
     6061 gcttaaattt ggcacaccgt tcgcgctttt tttaatgctc ggcaataaca acatgattcc
     6121 tgagcctaac accagcccaa ttagcaaaat attattttta ataaactcca cagacttcct
     6181 ctcgatgatt tatttgttgt atatggaggc tattgccgca atcacaacac tatttttagc
     6241 tcatttatca aatggcatac aaattttaaa aagcgatttt acgtggaaat acgctaaaat
     6301 agtggcaatc tctctaaaat attcaaacta aatttctcaa gcaattaaag gtgaaaatca
     6361 tgttacccgc agccaatctc acacccgtgt gcttactcat tttagacggt tttggttatc
     6421 gcgaagagtc agccaataac gccattgccc ttgcgcgcaa gccaaatatt gatgcccttt
     6481 ggaacaacat gccacacacg ttaattaatg cttctgagca ttacgttggc ttgccagatg
     6541 ggcaaatggg taactctgaa gttgggcatt taaacattgg tgcgggtcgt gtggtgtttc
     6601 aagattttga gcgcattaac aacagcatta aaaccggtga gttttttgag catcaagccc
     6661 ttaaatcagc gttgttgaat attaaaaata acaataaagc gctccatatt ttagggttgt
     6721 tgtctgacgg cggcgtacat agtcaccacg agcatatcta cgccatgctg gaaatggcta
     6781 aaaaactagg tttaaccaac gtatacgtgc atgtattctt agatggacga gacacgccgc
     6841 ccgtaagcgc agcgccttat attgcagcgc ttgaagcaaa aattcaggcg ctaggcgttg
     6901 gcaaaatcat ctccgtcagc ggacgttttt acagcatgga ccgcgataaa cgttggccgc
     6961 gtgttgaggc agcttatgca ctcattaccg agggtattgg tgaatttact tttgcaacag
     7021 ccaatgaagc cttgcaggcc gcctacgcac gtggcgaaaa tgatgagttt gtaaaatgca
     7081 cagccattcg caaacccaat gaagctgcca cgcacttaga agatggcgat ttagtggtac
     7141 acatgaattt taggtcggac cgtgcacgtc aactcacgca ttcattatta agtgaagaat
     7201 ttgatggttt tcatcgtcgc catgtgccag cattatcagg ctatttcaca ctcaccatgc
     7261 atgatgtaaa agaaaccaaa gccacggcga tttttccacc ctttgcggtt aaaaatacct
     7321 ttggcgaata tctttcaagc ctagggttaa cgcagttacg cattgctgaa actgaaaaat
     7381 acccgcatgt caccttcttt tttaatggcg gcgaagaaaa agtgttcacg ggtgaagacc
     7441 gcattttagt gccatcacca caggtagcca cctacgatat gcagccagaa atgagcgcgt
     7501 ttgaattaac caaaaagctt gaagaagcaa ttttgagccg aaaatacaat gccattattt
     7561 gtaactacgc caatggcgac atggtgggcc acaccggcag cctgcaagca gccattgagg
     7621 cgattgagac gctagatact tgcgttgggc gcattgtgaa tgccatgcaa agcatagatg
     7681 gcgaagtcat tatcactgct gatcacggca atgcagaaac gatgtttgac gttgaaaaca
     7741 atcagccgca cacgcagcac accaccaata aagtgccgtt gatttatgtg ggtcgagaag
     7801 ccaccttggc cgatgatggc gctctctctg atttggctcc caccttattg cacctgatgg
     7861 gcgtagcgca acccagtgaa atgacaggta aaaatcttgt caattttaaa tcatcaaact
     7921 cacctaaata atgagccgaa gataaacgcc gatgcacgcc gattatatgc ttcatttcat
     7981 caacccttct ggccgtttgg catttttaca aaaagtgcca aagttcagcg ttgtgatgct
     8041 gatttttatg ctattgctaa cgccagccgc acatgccgcg aaaaagattg atgaatccaa
     8101 gcaagattta aacgaagtca aagcacgcat tgagtcgctc aaaaaagaac tcgaccaatc
     8161 gcaagaggca cataaagatg cggcagacgc gctaaaagaa agtgaagtgg cgattagcat
     8221 tgctaacaaa aaactttatg aaatcaacaa taaacagcaa gttaacaaaa aagaactcag
     8281 tcatttagaa aaacaatcat acgcaaccaa ccaagcgctc agtcatcagc aaaagcgcct
     8341 cagtagccag ttgtaccagc aatacacgcg tggcgagcaa agttacgcgc aaatcatttt
     8401 gcaaaatgag caaccaagcg ctattgcgcg tgatgtgcag tatttctcat atgtggctaa
     8461 agcgcgcgct gaactcattc aggatatgcg gcgcaattta gaaaaaatca atcaattgaa
     8521 tgatgaaacc gcctctaagc tcaaagaagt cgctgaactt caacaaaagc agttgcaaga
     8581 aaaaaaggcc ttagaaaatc agaaacaagc caagtctaaa gttgtgagct cactttctca
     8641 acaaattgcc gcacaacgca atgaaattca aaaactcaaa cgcgatgaga aagcgctttc
     8701 tgatttggtt gagcggcttg ccaaaattgt gccgccgaaa aagaaaattg tcaaaaaaac
     8761 aacgacgccc aaacctggca aacaagattc cgactcaacc aaaccattgc caaccaagca
     8821 aacagaagag gaagtcattg aaaacaatga tgaaccgagt gctgatgacg gccttgcagg
     8881 tgccaacttt gcggcactga aaggcaagct tcgactaccc gttcgaggtg acgtgaccaa
     8941 ccgatttggc tccacccgtc ctgatagcgg tatttcatgg aaaggcctgt tcatcaaagc
     9001 gactgaaggc gctgaggtaa aatccattgc cacgggtcgc gttgtatttg cggattggct
     9061 gcgcggtttt ggtaatttaa ttattgtaga ccacggcagt ggctacatga gcttatatgg
     9121 caacaaccaa gccgtgctca agcaagtagg cgacaccgtg caaacgggag acgccattgc
     9181 ctcagtaggc aacagcggcg gcaatgaaac caatggactt tattacgaac tacgccgcca
     9241 aagtaagcca tttgacccgt tgagttggag caaactcaac taagtttaaa acgcaataaa
     9301 aaccgcctgt ttttgcccga gcaattttaa ctgccctgcg agcgtttttt tacaaggctt
     9361 gcagggtagc tcaaaagggt tgcatggatt tacataagca aatgcctatt tcattattta
     9421 attttcatcc cttatttgcg tattgactac atctaattcg cagtccaact agtcagttca
     9481 aaaacaaaat caattcaaga tgcgcatttt caaagcgcgt gttttttcaa tacattttca
     9541 aaagaactgt tgacaacagt tctacattta tggaaatata gtaaccatgg agactaaatc
     9601 tgctgttaaa ttactttcat ccattgccca ggaagcccgt ttggatatat tcagactgct
     9661 tgtacaagca ggttctgatg gtttggctgc gggcgtgatt ggagaaaaac ttcaaatccc
     9721 acccagtaca ctgtcatttc atctaaaaga gttaagtatt tccgggcttg taaaatctcg
     9781 ccaagtaagc cgttttattt attacgctgc gaattatgaa gcgatgaatg gcttacttac
     9841 ctacctcaca gaaaactgtt gtgctggcaa tgctgcatgt tgcggcaata cgacttgtga
     9901 tgattccatt aaaaattaat gatttgttga taaaaccatg aacattctat ttttatgcac
     9961 tggtaattca tgtcgctcaa ttttaggtga agcgactttt aatcatctag caccagcagg
    10021 ttggcgggcc atgagtgcag gcagcaaacc cgcaggttat gtgcatccac gctcagtggc
    10081 attgctcgcg cgtgaaggta tttcaaccga aggctattac agtaaatctt gggagagtct
    10141 gcctaccacg ccagatattg tgatttcagt ctgcggtaat gctgcaaatg aaacttgccc
    10201 agcttatctt gggcctgtac ttagaagtca ctggggcgtt gaagacccag ctcatgcaac
    10261 agggaccgat gaagaaatcg acgcagcttt catgaaagct taccgcattt tacgcgcgcg
    10321 tatcgaaacc tttttagcgc taccgctaga aacattaaaa cacgacaaac cagcactcaa
    10381 gcttgcaatg gataagattg ccaccatagt agtgtaagga gacaaggtga tgaaaacgat
    10441 acaagtattt gaccccgcca tgtgctgtag tagcggcgtt tgtggggtag aggttgacca
    10501 acagttagta agttttagtg cagatgtaga ttgggccaag cagaatggtg caaagattga
    10561 acgttttaat cttgcacaac agcccatgga ttttgcaaat aacaaagtgg tgagagattt
    10621 tcttgagcgt tcaggccaag aagcattacc gctcatttta gtggatggtg agtttgcttt
    10681 ggcaggacgt tacccaaatc gcactgagtt atcccgttgg gctggcatcg ttgcaataac
    10741 agaaattgcg cctgttggcg gatgctgtag cggcggtaaa tgttgctaat ttaaatgaag
    10801 gaaatcgaat gaaatttctt gaccaagcac cacgctttct attttttaca ggcaagggtg
    10861 gcgttggcaa gacttctatt gcctgtgcaa cagccattca attggctgag gcaggtaaac
    10921 gcgtattact ggttagtacc gaccccgcct ctaatgttgg gcaagtattt ggaatcacca
    10981 ttggcaacaa tataactgct gtgccagcag ttcaaaattt atcagcgctt gaaattgacc
    11041 cacaagctgc cgcgcaactc tatagagacc gcattgttgg ccctgtgcgt ggcatactgc
    11101 ctgatgcggt agtcaaaggc attgaagaac aactatcagg tgcatgcacc accgagattg
    11161 ccgcatttga tgaatttact tctttgctca ttgactcgcc actcactact ggctatgacc
    11221 acattatctt tgacacagcc cctactggcc ataccatccg cttattgcaa ctgcctggcg
    11281 catggagtgg ctttttagaa gaaggtaaag gtgatgcctc atgtttaggc ccattagcgg
    11341 gccttgaaaa gcaacgcgag caatacaaag cggcggttga tgcccttgcg gatgaaaaac
    11401 gtacccggtt aattttagtg gcacgtgcgc agctatccac tttgcgcgaa gtggcgcgta
    11461 cacacgagga gcttgctggc attggtttaa aacaacaata tttagtggtt aatggcattc
    11521 tgccaaaagc tgaggccgaa aatgatgcgt tagccgcagc aatatataaa cgcgagcagg
    11581 ctgctctaaa tgctattcca gaagtcttaa aatcgctccc gcaagataat gttatgctca
    11641 aaccatttga tttggttgga ctagaagcgc tacgtcaatt gctcgttgat acaccgcatc
    11701 atgcacaggt agtacaaatt tcgcccaaag tgttggacaa tcctagctta tcaaaactcg
    11761 tcgatgaaat agccgaggat ggtcatgggt tagtcatgac catgggtaaa ggtggcgtag
    11821 gtaaaacaac catagccgct gcgattgcgg ttgaattagc ccgccgtggc ttacctgtac
    11881 ttctcacgac ttctgaccca gccgcacatt tatcagacac ccttaatggt gcgctaaatc
    11941 atttaacagt cagtcgtatt gacccgcatg aagaaactga acgctatcgt cagcatgtat
    12001 tagaaaccaa aggctcaaaa ttagatgcgc aaggtcgcgc cttgctagag gaagatttgc
    12061 gctcaccgtg taccgaagaa attgccgtat ttcaagcatt ctctcgcatc attcgtgaag
    12121 cgggtaaaaa gtttgtggtg atggataccg cccctacagg gcatacgcta ttattactag
    12181 atgcaacagg ggcttatcat cgcgaagttg cacgccaaat gaataaatcg cacttgcact
    12241 acaccacccc gatgatgcaa ttgcaagatc caaaacaaac caaaatactc atcgtcaccc
    12301 ttgcggagaa aacgcctgta cttgaagcgg cgaatcttca agctgaccta cgacgcgcag
    12361 gcattgagcc ttgggcatgg gtgattaaca acagcgttgc agccacttcg actcactcac
    12421 cattgcttca tcaaagagca ctaaatgaat tagtggaaat taattcagtc gcgcaacaac
    12481 acgcaaaaag atatgccgta ttgccaatgc ttaaagatga accaagaggg ctgacccgat
    12541 tactggaatt ggctggtaac aagcatttgt agtgatgatt tgctctttgg gtgactcatt
    12601 caaccctgca taaaaattaa tcaagactat aataaccctt tttaagtcca ctttttaaag
    12661 gatcgcatca tgaacagaag agaagcttta ctgagcggcg cagcgattgc agcagcggca
    12721 gttgtgggtc gtgcgcaagc cgcagaaatg caacatcacc accatgcagc aatggccaat
    12781 accgccctag tatctgcggc ggcagattgt attcaaaagg gccaagtctg cctgaatcac
    12841 tgcctgtacc tgcttgggca aggcgataaa gaaatggccg catgtgcaaa aagtgtcaac
    12901 gaaatgctgg ctttatgtgg cgcactccaa tcactgtcca atcaagagtc agccaccctg
    12961 ccacggcttg cagccattgc tatggatgcc tgtaatcaat gtgaagaaga atgtaaaaaa
    13021 catgcggata agcacgatgt ctgcaaagcc tgcggtgaaa gctgtaacgc ttgtgccaaa
    13081 gaatgtaaga aaattgccgc ttaattttta gctttaaatc tcaaagccag ttttgcgtat
    13141 ttttttaagg ctaatttagc tgttaatttc tacagcgcgc ctaagtaaaa atctcacgcg
    13201 ctggtaaatc ttccttgctc agcatccgcc aataatgtga ataaatcttg actgttgagt
    13261 gacagcgcat aacccaatac gccactgcca atgacgacga ttgggtaatc tttcacactg
    13321 ttatccacca aaattttaat ctcactcggt aatgctatgg acggaacggc gccgacaata
    13381 tagcccgtgg cagtttgcac ttcatcaaat tctgccatgc ggcacttgcg cgcatttaaa
    13441 tgttggcgta aaactgccca atccgcacgg ccaccgcccg ccaccgctaa cagcacaaat
    13501 tgttcagacc ccgctttaaa caacacgctt ctcaccaccg attgcgggtt aatgccttgc
    13561 tgggtgagca aggcttctaa attgcgaatc ggctttttat cttcagtcag cggaatttct
    13621 atcacgctaa aagcaatgcc ttgttgcagt agcagttctg taacgggaga agtgattaaa
    13681 gcgctcattt tttaaacttt caaaattagg ttaaaaaatc cacttgatat tctaatgtta
    13741 aaaagtaaaa ttactgcaat caattcaaaa ggtttgtcat gaaaaatagt gcaatcattg
    13801 tgaatacggg tggcggttac aataatgtca ccttcggtca tcgtgaagca accgcccctc
    13861 gcgccggtga aattaaagta cggctgcacg ctaactcact gaattatcat gattttgcgg
    13921 tggtaagcgg catgtggggg ccaaccgagc cacgcattcc aatggcagat ggtgcaggcg
    13981 ttgtattaga agtaggagaa ggtgttactg agttttcggt gggtgaccat gtcgttagca
    14041 cgttttttcc tacttggttg gctggtgaac ccttggtgga aggctttgct accacccctg
    14101 gcgacggggt ggatggctac gcacgtgagg ttgtaaccgc catggccaac gcttttacac
    14161 tagcgccaaa aggctggagc cacattgaag cctctaccct caccacggcc gcattaacgg
    14221 catggcgcgc tttaatgtct gacgatgcgc taaaacctgg cgacacggtc ttgatacaag
    14281 gcactggcgg cgtttctatt ttcgctttgc agtttgccaa aatggccggt gcaacagtca
    14341 ttgccacatc atcaaatgac gagaaattag aaagactcaa atcattcggc gccgaccatc
    14401 tgattaacta taaaaaaacc ccgcaatggg gaaacgtcgt acgtgaaata accaatcatc
    14461 aaggcgttga ccatgtcatt gaagtgggcg gcccagccac actggaacaa tcgatgacgg
    14521 cggcgcgcat tggcgggcat atttcagtga tagggatttt aacggggcta ggcggggaga
    14581 tttcaattgt cagcgccctc attaaacaac tgcgtcttca aggcgtgctg gtgggcaacc
    14641 gcacgcaaca gcaagaaatg attaaagcta ttaatgctaa caacatgcgc cctgtgattg
    14701 acaaggtttt tgcattagaa aatattgtgg gggcttttcg gtaccaagaa accaaccagc
    14761 attttggaaa aatttgttta gaaatgtaat tgcccatcca cttgtgaaaa ccaactggcg
    14821 ctaattttta gggctgagtc acgtcgctaa tagtgttcat cataagtata agtgctcact
    14881 tctttatcga tgagttttgt gattttcttg atgtttttct tgaatttagc tacgtttgaa
    14941 acaccgccta aatgggtagt ttcgcgttga acatgcactt ccaaaccatt gcggccttcg
    15001 ctacctctaa agttttgcac cgccaacgtt aatttttcca cttcattcac gttaaatatc
    15061 aatgcaagtg ttgcatcggc aaagccaatc acattctgtt gtgtatcatg ccaaatcaac
    15121 agcggctgcg cttcagtaat catcggctta aagcaatctg gcgtataaat ggcattttca
    15181 acttgccgat aattagcatt taaatcaatc gcaaaatcaa acgtagcata ttgaatggct
    15241 gcatttagca ccaaattacg ttgataatca gtgcggtaaa agcggtctag atttggcgca
    15301 taacgtatgc gtaaccacgc cacgttatcc caaccatcgg gcatttcgcg ataaaaacaa
    15361 taaagctcaa cgctgaccga ttgaacttgc caagtgacgc gccaagtgtc tttgacgctg
    15421 taatcaattt ggctaagcgc gttattttga aaaaaatgat ctaaatgcgt ttttatggca
    15481 tcaaaacttt tttgtcggtt tgcgccaagt gaaatttctg cgcaatattc aatcgcgggc
    15541 aaatgcagtt tgagtgcgcc ttgtgcgcta tcgcaagcgg tataaaggct nnnnnnnnnn
    15601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgggcaaatg cagtttgagt gcgccttgtg
    15721 cgctatcgca agcggtataa aggctgtgaa gttttaaccc attaaaaatg gtcggttttg
    15781 taatgcaata ttctgtggca tgaaaagatg gattgggaaa atcttttcgt tgtgttttaa
    15841 tatgcttatt gttttgtaaa tcttcgtaag tgccccaagc aattaaggta ttgagatgtt
    15901 caagccacaa gccttgttgt agcaaatcca gtgcactttt tccgttatca gcaacatctg
    15961 tcggcgaaat cgcaaaatct gccgtgtgca ctttttgcca aagaactgct tcagccatgt
    16021 ccaccttggc gtttctaaag gccgtataac gctcaacatc aaagtttggt aactgggtga
    16081 caatgcgctc taaggctgca aaattgcctg ccaacgtaca aacgctaagc gcctgagcgg
    16141 aatatgatcg caaatttagc cagcagcggt catgcgcgtc aagattatca aaccgataaa
    16201 tcgtgatgta atcgacgtca ttcaagtgaa tctagctgtt atcatgatga tggcgaatga
    16261 ggcgaaagtg atggccgtca atcaccaaac gctcaccata aaatgggtgt tggtcaggcg
    16321 ctttttctgg tatgaaaatt ttgcttatga atgaaaacat cgcacttcat tgcttaaaaa
    16381 attggttaaa aaaataggaa tatgaagtgg tttaatttaa cttaaattgt tgctttaact
    16441 gttgcttttc aatacgagaa aaaaataatg ctctgcgagc ctttatttgc aaggctcaca
    16501 gagcatcaaa aaaggttgta acttaaattt acattttttt ggataaaatc cgacatcaaa
    16561 tacgtcgcca agtggtgcct tgtggactgt cttccaacac aacacctgcc tctaacaatt
    16621 gcttacgaat ggtatccgct tcagcaaagt ttttggcttt ttttgcttct gcacgcgcct
    16681 gaatcagggc atcaatatta agcgtttgcg ttgcctcacc ttgcaaaaac gcatcggggc
    16741 tacgttgcaa caagcctaat acgcctgcta atgatttaag caatccggca gtctcggcag
    16801 atttcgattt atttacttcg ttggccaaat caaacaacac ggccatggct tctggggtgt
    16861 tgaaatcatc atccatcgcg gctttaaagc gtactgcttg cggatgttgc caatcaatcg
    16921 tattacttgc aacgtcgtgg ccacgcaagg ccgtgtacaa gcgagtaagc gctaacttcg
    16981 cgtcatctaa atgctgatct gaatagttca gcgggctacg ataatgcgcg cgcaaaatga
    17041 aaaaacgcac cacttccgca tcgtattttt ctaatacggt tctaatcgta aaaaagttgc
    17101 ccagtgattt cgacatcttt tcatcgtcca cgcgcacaaa gccgttgtgc atccagtaat
    17161 tgaccatttg gcaaggctgg ccatcatggc tgtgcgtggc ttcgccattc catacgccgc
    17221 cgtgcgcggc ctcagattgg gcgatttcat tttcatggtg cgggaactgt aaatcttgcc
    17281 cgcccccatg aatgtcaaaa tgcgcaccca ataaatgcga actcatcacc gaacattcaa
    17341 tatgccagcc gggacgacct ttgccgttat tcggcccacc gaatggtgaa tcccaactcg
    17401 gttcattggg ttttgccgcc ttccaaagta caaaatccat cgggtcttgt ttaaagctgt
    17461 caacttccac gcgttcaccc gcgcgcaagt cttcaagcga tttacccgat aatttgccat
    17521 aaccgtcaaa tttattgaca gcataaaaca catcgccatt cgcggccgta taggcaaacc
    17581 ctttgttaat taacgtttga atcatctcca ccatgccgct tacatattcg gtagccttgg
    17641 gctctagcgt tgggcgtatt acccccaatc tggctgaatc ttcatccatg gcgtcaataa
    17701 agcgctgtgt aagctggctg attgtttcat tattttcatt ggcacgttta atgattttgt
    17761 catcaatatc agtgatatta cgcacataag tgacctcgta atcagaagta cgcaaccagc
    17821 ggcttaccat atcaaacacc accatcaccc gcgcatggcc taaatgacag aagtcataca
    17881 ccgtcatgcc gcacacatac atgctgactt tgccagcctg tataggggta aaaacttgtt
    17941 tttcacgtgc gagggtgttg taaattttga gcatagtaat gatgaaaaat cttaataatg
    18001 agtcaaacga taaaataacg ttaaaaataa aatgttcgct aattttaaag taaaatggcg
    18061 cctgacttga aattatctgc aaccgttgcg gtctactttt tacattttat tttaactaag
    18121 gacatgtatg cgtcatcttt ttgctctctt aatgttggta tttttcagcc acagtgcact
    18181 agcggcagat actaaaacaa acccgcaagt cacatttgaa accacacacg gcaacattgt
    18241 gcttgaactt tacccacaaa aagcgccaaa aacggtcgcc aattttctgc aatatgcaga
    18301 gtctggcttt tataaagata ctatttttca ccgtgttatt aacggtttta tgattcaagg
    18361 tggtggtttt acggcagata tgcaagaaaa accaaccaac gcacccatta aaaatgaagc
    18421 tgctaacggc ctcaccaatg acaaaggcac cattgccatg gcacgcacgt ctgaccctga
    18481 ctctgccact gcgcaatttt ttattaattt agtcaacaat cacgcactta attatcgcgg
    18541 tgatagccca gaagaaattg gctattgcgt ctttggccgc gtattgacag gcatggatgt
    18601 ggtgcgcgaa atcgccattg tcaatacggg taacgctggc ccacacggca atgtaccaaa
    18661 atcacccatc atcattaaac aagttaaaat cgttaaatag gaacattcaa catggtaaaa
    18721 ctacaaacca atttcggtga catcaccatt gaactcaacg ctgagaaagc acccattacg
    18781 gtcgccaatt ttttacagta tgttgataat ggcttttatg atggcac
//
LOCUS       DAOQSI010000020        18524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_2112_length_18524_cov_6.309275, whole genome shotgun sequence.
ACCESSION   DAOQSI010000020 DAOQSI010000000
VERSION     DAOQSI010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 18524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18524
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2112_length_18524_cov_6.309275"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            663..1520
                     /gene="rpoH"
                     /locus_tag="PKL42_02620"
     CDS             663..1520
                     /gene="rpoH"
                     /locus_tag="PKL42_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HNU65994.1"
                     /translation="MSNALTLPALTAIDSVEHYARAIKAYPILSAEEEYALAVKFKQE
                     NDLEAARQLVVSHLRLVASIARGYIGYGLPQADLIQEGNIGLMKAVKRFDPDRGVRLV
                     SFAMHWIKAEMHEYIVRNWRLVKIATTKAQRKLFFNLRSMRTGLNTLQPKEVAHIAKT
                     LNVKPEEVLEMEARLNGHEISLEADHDDDSDEHYSPIAYLQDESLEPSDALQAKQAEI
                     AETTGLAQALQSLDARSRRVVEARWLQDAGGQTLHELADEFGVSAERIRQIEQKAMQK
                     MKTLMLASQ"
     gene            complement(1754..3715)
                     /locus_tag="PKL42_02625"
     CDS             complement(1754..3715)
                     /locus_tag="PKL42_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HNU65995.1"
                     /translation="MSENKLAHQFAVYSEWRKALVDTICDYRSWLNEQELNDSQSDQR
                     IAQLLERLREDKLNVAFVAEFSRGKSELINAIFFAGYGKRLLPSSAGRTTMCPTELLY
                     DKSKPTSIMMLPIETRLSDATTTEYKRYPDEWVVAKFDVENNDSMVTAFKEVSNTKAV
                     TLEEAEKYGLFDPNSADDALNINKDGTIDVPCWRYAIINFPHPLLEQGLVILDTPGLN
                     AIGTEPELTLNMLPNAHAVLFILGADTGVTKSEIDVWRQYISGTRWKQKGRLAVLNKI
                     DGLWDELKNEKDIQTELTKQIKTSAELLGLETTQIFPVSAQRGLLAKIHNDAALLEKS
                     RILELEKALSDELIPSKKEIVRDNTQNEVEDLISNSNMIVDARLSGIVEQLNELRGLR
                     GKNEDVVEHMMNKVKVDKENFEKGLQRFQALRSIFSQHTNRLFTLLGMEALKTHVHTT
                     RETMVAANFTKTIRGSMDNFFSELRTRLISSDAQIEEIKKMMDVMYDKFSKDHGLRKT
                     EPPAFSTLRYQKELAKLERAYKEQFNTTFNMIANEKMTLTSKFFETLASRVIHVFEVA
                     NRDIENWLKAVIAPMESQVREHQLQLRRRLESIKRIYKATDTLEDRILELETIEKSIQ
                     AQLDDLQVLRMHMKNALAFEAEVEEAVAA"
     gene            3986..4363
                     /locus_tag="PKL42_02630"
     CDS             3986..4363
                     /locus_tag="PKL42_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HNU65996.1"
                     /translation="MSGLTQHSPRPIDIRLHQASRLLEIKFDNHTECQLSCEFLRVYS
                     PSAEVRGHGHGQEVLQTGKEEVNITNIEPVGNYAVKLTFSDGHNTGLYSWDYLYELAQ
                     NYEALWLEYLGKLSVAGIQRQST"
     gene            4384..5151
                     /gene="ubiE"
                     /locus_tag="PKL42_02635"
     CDS             4384..5151
                     /gene="ubiE"
                     /locus_tag="PKL42_02635"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HNU65997.1"
                     /translation="MTTEHDSNQQTHFGFKTVHEAEKAQKVGEVFHSVASKYDLMNDV
                     MSAGLHRSWKRFAVEVSGVKTGDKVLDIAGGSGDLSKLFAKKVGVGLPQNAGKVILTD
                     INASMLGVGRDRMLDAGLNVPALQCDAEKLPFPDQYFDCVIVAFGLRNMTHKEVALAE
                     MQRVLKVGGRLLVLEFSKVWQPLSKIYDAYSFKLLPLMGKIFAKDAESYQYLAESIRM
                     HPDQETLKQMMTDVGFFKVDYYNLAAGVVALHKGYKT"
     gene            5151..5765
                     /locus_tag="PKL42_02640"
     CDS             5151..5765
                     /locus_tag="PKL42_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU65998.1"
                     /translation="MLKQLSTRLLQHLIAQNSWANALLHPYVGQSVRINIGLISPTVV
                     ILEDGSLAMAGDTNTPEATVTIPPSLLLRLLAKDESAKNQIQITGDTHLATELAKVFA
                     HMRWDYEDDLSKIVGDIPAYKIADFGRSAVGSIKDTSTNLAEMLSEYWQEEMPVIAKK
                     RHVAQFNTEVDTLRADVARFEKKLRKLEKTLANPANDQPETQHS"
     gene            5773..7320
                     /gene="ubiB"
                     /locus_tag="PKL42_02645"
     CDS             5773..7320
                     /gene="ubiB"
                     /locus_tag="PKL42_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HNU65999.1"
                     /translation="MRIFRLFYIIFIALRFSLDDLVLSHTKLKPLQSVIRILLFWRNT
                     KKPRGERLRLALEALGPIFVKFGQMLSTRRDLLPLDIADELAKLQDQVPPVAFTEIEA
                     VIHEAFGLPLNQVFSEFETTAIASASVAQVHFAHLMDGTPVAVKVLRPGIAKTIQHDL
                     ELLDTGAWLLQALSTEGKRLKPREIVAEFAEHTHSELDLTLEAANCTRLAANFTDKKL
                     LIPAVHWDWCRQQVMVMQRMVGTPISKIEELRAKGIDIAKLAHDGVEIFFTQVFRDGF
                     FHADMHPGNIQVATDGEYKGRYIALDFGIMGTLTDNDKYYLARNFLAFFNRDYRDVAV
                     AHIESGWVPKDTNVEALETAVRAICEPIFNKPLKDISFGRTLLSLFQMSRKFGVVIQP
                     QLVMLQKTLLNIEGLGRDLDPNIDLWQTAKPFLKRWMSEQIGWRSVANTVKKELPFIV
                     KNLPQMPRLVHQFLQQQTQAEQEAPMRAALDALIKVQEKQARWQKRLTFALLILIALQ
                     IASFVLH"
     gene            7571..8422
                     /locus_tag="PKL42_02650"
     CDS             7571..8422
                     /locus_tag="PKL42_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spherulation-specific family 4 protein"
                     /protein_id="HNU66000.1"
                     /translation="MIQKKRIIQFALLSANMLMLNAHATEILVPAYFYPSSDPNLSFW
                     DEMTAAASQGVGITAIMNPNSGPSTAENSDYTNAVSTFRAAGGKVIGYVPTGYGARDA
                     SLVLADVSAYKSFYQIDGIFLDEMHNIASELTYYESLRTGISGIDVNYKVFGNAGTNT
                     LEDYMHTADVLVTFENQTGYTAYAPDSWTGNYTANRFAHLLYNVGTAADMQAAITLAA
                     SRNVGYIYVTNDNGANPWDTLPSYWNAEVTGVSAVPEPETYSMMLLGLGLIGWQYKRR
                     NALKTSD"
     gene            complement(8425..9240)
                     /gene="mutM"
                     /locus_tag="PKL42_02655"
     CDS             complement(8425..9240)
                     /gene="mutM"
                     /locus_tag="PKL42_02655"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HNU66001.1"
                     /translation="MPELPEVETTRLGLLPLLGKVVQAVEIRHPTLRWPIPQHLTQTL
                     PHQTLHKLTRRAKYILCAFDTGTLLLHLGMSGRIQLLDRHYPPEKHDHFDIQFSDAQV
                     LRLRDPRRFGAVLWLDNQNHDHALLNQLGPEPLEASFNAKYLHQTLSSKSLAIKNAVM
                     DGHVVVGVGNIYASESLFRARIHPETPAKNLSLKQCERLVTEIKNTLQDALNAGGSSL
                     RDFFGTDGNPGYFQQEYFVYGRTDEPCKVCAKPIQCIRLGQRSTFFCAHCQKR"
     gene            complement(9259..10170)
                     /locus_tag="PKL42_02660"
     CDS             complement(9259..10170)
                     /locus_tag="PKL42_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNU66002.1"
                     /translation="MKKRWLLNLVLLCAVASLVAFLYLRPKENVQQAEVYELSNYKLA
                     EFTAISVEPPTKAPVTFAKVDGFWRLTAPTKARADQNAVARILSIIAARSKEKVLVDS
                     ADTASLEKFGLTNPKLQLKLIRADQSVARFIFGTHNPINDAQYVLLENAVYLIDNAYA
                     EAATTQVIELIDKAPLKPTEKVAGFDLSRLEQWEEAKLNVDLVEGKWKVSIPAAKPQQ
                     DELNEWLEFSWVKTAAKSVEFYTPDPKAKYPSLEIKLADGSRVHFDKLQESPDLVLAR
                     PDEGLIYNFQSDIGFTMLNPPINLPSN"
     gene            complement(10362..11747)
                     /locus_tag="PKL42_02665"
     CDS             complement(10362..11747)
                     /locus_tag="PKL42_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HNU66003.1"
                     /translation="MNINRKLRFQLFVQNGFFVVLFLLLVILLGYLASQYHVAKDITQ
                     ANRNILTQGSVNVLKQMKGPINITVFATKDDASSGDNFRKGMIDFVARYQREKKDVNL
                     TFINPSIEPKLAQEAGVKEDGEVVVEYQKRVEHINPPIAEQEMTNLLVRLSRTNQQAV
                     MYLDGHGERNLLGVRNHDLGEFGKQLEKKGFKFANPDLTIAQAVPSNGAMLVIASPQV
                     DVSEIEAKKIKTYLESGGNLLWLLDDNNLRGLDQVAEYLGMQVSPGIALDMASAQYGA
                     DARVAFASLYGEHPITTNFMLRTLFPEAHQVTAKGTDENGWKVSNLVEVAPNGWLTAD
                     KLAKDAKPKFNEKTDKRGPINIGVALERIYGKKGQRVVVMGNANFLSNTFITNGGNLD
                     LGVNIVNWLAGDDNLITIQPMPLKDINVTIPDSDTGRLVAWTVFHGFQFFFPIGMMIA
                     GFYFWWKRRKA"
     gene            complement(11805..12551)
                     /locus_tag="PKL42_02670"
     CDS             complement(11805..12551)
                     /locus_tag="PKL42_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNU66004.1"
                     /translation="MIINVARKELKSMFSSPMGWVILSLLMFAFGTYYLNGVNDYFAV
                     MSGAMRPAERTGVTQFVGQTVYGFASFIVLFAVPLLSMRLISEERRNQTLPFLFSAPI
                     SLTEIVIGKFLGLVLFLSILIVYIGLMLSTLNIWADIDFGYILANSLGMLLMVASFSA
                     LGLYFSSMTQQPVVAAILTFIALFALMFLDRFFAGDPSNTLASLSLTRHFQSFASGLI
                     DTADIIYFVLFIATFLTLTVRRLDADRLRG"
     gene            complement(12594..13526)
                     /locus_tag="PKL42_02675"
     CDS             complement(12594..13526)
                     /locus_tag="PKL42_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNU66005.1"
                     /translation="MNNYTVEAIELTRLYGGREAVSNVSFTLSKGQVLGFLGPNGAGK
                     STTMKMLTGNLAPSQGSVKICGIDMIENPKEAKALIGYLPEMRPLYKELTVDEYLTIA
                     ARLHRVSAKHIKPAVEEAKERCGLSHMSKRLIENLSNGYQQRVGIAQSIIHKPMVVIL
                     DEPTVGLDPIQIRDIRALIREIGSDHSVILSTHILPEVEMVCDHVQIIDKGKLVFNGA
                     IEVLKKQRIGNKLLIAMRNPPTVEAMLSIPNVVEAESLPNGQMRVRFADDTAPAESIV
                     QAAVSNGWGLYQIAPDQTSLEDVFVQLTYQEQAA"
     gene            13897..15681
                     /locus_tag="PKL42_02680"
     CDS             13897..15681
                     /locus_tag="PKL42_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNU66006.1"
                     /translation="MRKYIGLQTNKTKTTSRYTKAFYAGLIVSTISLSGCATNKPVSD
                     TLASSNKPQPTQTGLSAEFVYKYLVAEIAGQRGDFATSGAAFYDLARTSRDPSLAERA
                     AKIAAYGKVGGLAIPAVKLWAELDPSSNEAQQAMTELYIATGKLNEAKPFLAQLLVKE
                     ETRAGGFLYLNTLLGKSPDRTAALKLVQSLAEPYPELAEAQVAIAQAAWAAKQNDATL
                     NALTKAETLKPGWNIPALLKGQVLFEQSPEAAIKFYQDFLVKYPQSNEVRLNLAKILV
                     NQKQYDAAKKHYPIIIQQAKEEPEKNTAEVTALIGLLSSQSGDYAAAESYYKQSIELG
                     FKNPDQLHIYLAQAAEKQKQDKSAIDWYNKIQPASAHYLEAQLNLANIIARTESADKA
                     IEKLDTIDDLTTEQQIIVIQSQASILAKAKRDQEAFDLLDKAVKNMPNTSELVYDYAL
                     AAERIKKFDLMEAELRKSIAVKPDFAAAYNALGYSFADRNIRLNEAIKLIEKALSFSP
                     NDHYMLDSLGWAHYRKGNLDKAITYLQQAYNTSQDPEIAAHLGEVLWKKGKRDEANKI
                     WSDALTANPENEVLINTTNKFKTIQSNS"
     gene            15691..16320
                     /gene="lolB"
                     /locus_tag="PKL42_02685"
     CDS             15691..16320
                     /gene="lolB"
                     /locus_tag="PKL42_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HNU66007.1"
                     /translation="MVSIQSTQWQQSIRIALVIGLMAFLQSCVSVPAIKPAESAASKA
                     LYLKHQQEIAAIQQFSLQGRIGVQSENKGFSGGLTWQHDNANDDISLFSPLGGQVASI
                     KKTATQVTLVDDKGNSISEVDAETLTQKTLGWQLPLSGLADWALGRPSSNHIMNSTWD
                     EQGYLITLKQDGWDIKYENYSEQNGRFLPRKILLKNEKVSLKLLIEEWN"
     gene            16427..16948
                     /locus_tag="PKL42_02690"
     CDS             16427..16948
                     /locus_tag="PKL42_02690"
                     /inference="COORDINATES: protein motif:HMM:NF038126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxDxF family PEP-CTERM protein"
                     /protein_id="HNU66008.1"
                     /translation="MKKLSKYLSVGLLVAASAASFTVNAAVVNLGNLSGTYANTFERS
                     FGPFIDNIKFKITTPSNLGSAANHLDLSFNIPGIGNVDLYNITGMYYNITGPGSFTTG
                     TLSADNTTHSTILNVAGDYQVNLFGNATGTKGGLYAVALNVTAVPEPETYALMLAGLG
                     LVGFAARRRKSAV"
     gene            17147..18481
                     /gene="miaB"
                     /locus_tag="PKL42_02695"
     CDS             17147..18481
                     /gene="miaB"
                     /locus_tag="PKL42_02695"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNU66009.1"
                     /translation="MNTPKKVFIKTFGCQMNEYDSSRMADMLSAENGMVETLTPEDAD
                     VILLNTCSVREKAEDKVFSHLGRFIPLKKANPNLVIGVGGCVASQEGDNIIKRAPYVD
                     VVFGPQTLHRLPEMIKSSQSSGQSQVDISFPEIEKFDHLPPPRVEGASAFLSIMEGCS
                     KYCSFCVVPYTRGEEVSRPFEAILTEAIQLAEQGVKELTLLGQNVNAYRAEYQGTEAD
                     LALLIEYIAEIPQLERIRFTTSHPNEMTERLIACFANIPKLAAQLHLPVQAGADRILM
                     AMKRNYTALQFKSTIRKLRAANPNLTFTSDFIVGFPGETEAEFEATAKLMQDVSFDYS
                     FSFLYSPRPGTPASYLTDETPQSTKLARLAKLQALNEAQGKLISEAMLGTAQRVLIES
                     ASWKNASELAGKTDNNRIVDIVGDANLIGHVVNVRITDVSNPKRLRGEIING"
BASE COUNT         5344 a         4229 c         3897 g         5054 t
ORIGIN      
        1 gcattcatac aactcaatca tttttgcgtt tttacacagc ctgtccccaa cttacgcgtt
       61 cacttggttg gcaaaatcca aattataatg gccaccctta acaacactta gttgatcttt
      121 tgacattagt atccagcaat ccgtaatgca aactctgttt cttttaatta atttccacac
      181 aaaattggct gagcaattat ctgtatctgt atcaacccta taatttctcc tttagacgat
      241 accccattgc cttgtatcct ttcatacgtt tttcaaacat gcgcatgagt actggtcgtt
      301 ctcatcaaaa taatcataag tcagcaccac tttttttcct tcatgcaaac gatgtagacg
      361 ccagcatatt gcttcaatgt gccttgctaa tatatgggca ttgttaaaaa taaagtatcc
      421 aattgtgggt catcgaatcc ttcaccaatc aattttcctg tggcaagaat tagccttgtt
      481 gctccaacgg gtattgtcaa gttaatgact ttattgtcca gttaacgact ttaatgtccg
      541 taaacatgaa gtttcatcaa acaaaaaaat tcaccagtga actattcacc tctagcactc
      601 tcaaagctag agtgctaata tatgcatgcc tgtttaattg gctataggag gaaacaaact
      661 gaatgagtaa cgcattaaca ttaccggcat taaccgcaat tgatagcgta gaacattatg
      721 cgcgggcaat taaggcatat ccaattctgt cagcagaaga agagtatgcg cttgctgtta
      781 agtttaaaca ggaaaatgat ttagaagctg ctcgccagct tgttgtatcg cacctgcgtt
      841 tggtcgcgag cattgcgcgt ggctacattg gctacggctt gccgcaagct gacctcattc
      901 aagaaggcaa tattggcctg atgaaagcgg taaaacgttt tgacccagac cgcggtgttc
      961 gcttggtttc gtttgccatg cattggatta aagcagaaat gcacgaatac atcgtgcgta
     1021 actggcgctt ggttaaaatt gccaccacca aggcacaacg taaattattc tttaatttac
     1081 gtagcatgcg cacgggctta aatacgctac agccgaaaga agtcgcgcac attgctaaaa
     1141 cgttaaacgt aaagcctgaa gaagtgcttg agatggaagc ccgtttaaat ggccatgaaa
     1201 tttcacttga ggctgaccat gacgatgaca gcgatgagca ttacagccca atcgcttatt
     1261 tacaagatga aagcctagag ccttctgatg cattacaagc aaaacaagca gaaattgctg
     1321 aaaccactgg tttagcgcaa gccttgcaaa gcttggatgc gcgtagccgc cgtgtggttg
     1381 aagcgcgctg gttacaagat gctggtggtc aaaccctgca tgaattggct gatgagtttg
     1441 gtgtatcagc agaacgtatt cgccaaattg agcaaaaagc catgcaaaaa atgaaaacct
     1501 tgatgctggc aagccaataa tcaaatccat tatctgcact ctaaaaatca taaaggctgc
     1561 aattgcagcc tttatttttt gcatctaatg cttatccaaa atcaagttaa aacctgccta
     1621 aattaaccac aacctttttg atgcctctgc gagcctttat ttacaaggct cgcagggcag
     1681 tatttttttc tcggcaatat ttagcttaaa atttgtgtga tttctgagct aaaacaaccc
     1741 tatcatttca gatttaagcc gctaccgctt cctcaacttc agcctcaaat gccagcgcgt
     1801 ttttcatgtg catgcgcaac acttgcaaat catccaactg cgcttgaata cttttttcaa
     1861 tcgtttctaa ttctaaaata cggtcttcta aagtatccgt cgctttgtag atacgtttaa
     1921 tactttctaa gcggcgacgc aattgcaatt gatgctcgcg cacttgcgat tccatgggtg
     1981 caatgacggc ttttaaccaa ttttcaatgt cgcggttggc cacttcaaac acatgaatca
     2041 cacgacttgc cagcgtttca aagaacttgc tggtaagtgt cattttttca ttggcaatca
     2101 tattaaaggt ggtattgaat tgctctttgt aagcacgttc taattttgcc aattcttttt
     2161 gatagcgcaa ggttgaaaat gccggtggct ccgttttacg cagaccgtga tctttcgaaa
     2221 acttatcgta catcacgtcc atcatttttt tgatttcttc aatttgcgca tctgatgaaa
     2281 tcaagcgcgt tctcaattca ctgaagaaat tatccattga gccacgaatg gttttagtaa
     2341 agtttgctgc caccatggtt tcacgcgtgg tgtgcacgtg cgttttgagc gcttccatgc
     2401 cgagcaaggt gaataagcgg ttggtatgtt gcgagaaaat actgcgtagt gcctgaaagc
     2461 gttgtagccc tttttcaaag ttttctttgt ctactttcac tttgttcatc atgtgttcaa
     2521 cgacatcttc atttttgccg cgtaagccac gtaattcatt caactgctca acaatgcctg
     2581 ataaacgcgc atcaacaatc atgtttgaat ttgaaattaa gtcttcaact tcattttgtg
     2641 tattgtcacg cacaatttct tttttggacg gaatcaactc gtctgataag gctttttcta
     2701 actcaagaat acggctcttt tcaagcaatg ccgcatcatt gtgaatttta gcaagcaggc
     2761 cgcgttgcgc agaaacaggg aatatttgcg tagtttctaa gcccagcaat tcagcactgg
     2821 ttttaatctg cttggtaagt tcggtctgaa tgtctttttc gttctttaac tcatcccaaa
     2881 gaccatcaat cttgttgagt accgctaagc gacctttttg tttccagcgc gtgccgctga
     2941 tatattggcg ccacacatca atttcagatt ttgtcacgcc tgtatcggca cccaaaataa
     3001 ataacaccgc atgcgcattg ggcaacatgt taagcgttaa ttctggttcg gtaccgattg
     3061 cgtttaaacc cggtgtatct aaaatcacca agccttgctc aagcaacggg tgtggaaaat
     3121 tgataatcgc ataacgccag catggcacgt caatggtgcc atctttattg atattaagtg
     3181 cgtcatccgc gctgtttgga tcaaacaggc cgtatttttc tgcttcttct agcgtaaccg
     3241 ctttagtatt gctcacctct ttaaatgcgg tgaccatgct atcgttattt tccacatcaa
     3301 acttggccac cacccattca tctgggtagc gtttatattc agtggttgta gcatctgaca
     3361 acctcgtttc aatcggcaac atcatgattg aggtcggttt agacttgtcg tacagcaact
     3421 cagttgggca catcgtcgta cgcccggcgc ttgaaggtaa tagccgtttg ccatagcctg
     3481 caaagaaaat ggcgttaatc agctcagact taccgcgtga gaattcagcc acaaatgcaa
     3541 cattaagctt atcttcacgc aatctttcaa gcaattgcgc aatgcgttgg tcgctttgtg
     3601 agtcgttgag ctcctgctca ttaagccagc ttcgataatc acaaatggta tcaacaaggg
     3661 ctttacgcca ctctgaatat accgcaaatt gatgtgctaa cttattttct gacataacaa
     3721 tccttaaaac ttttacactt aatataactt tatcatggtt tattgaataa tcaaataaat
     3781 caatcaatta ttgccgacat ctaaaataaa attcagataa gcggttgtat ttaatctatg
     3841 agcggtcaaa catgaccaaa agtgagtcat tatcgacaca taatcaaggt ttttgtatgg
     3901 cgccaaactt gcaaacttat agaagctagc ctgcacattc tgttatcatt tctactttaa
     3961 attgcacggt tttacatgtg aaataatgag cggtcttaca caacattccc cacgccccat
     4021 cgacattcgc ctgcatcagg cgtctcgatt actagagatt aaattcgaca accatacaga
     4081 atgccaattg tcttgcgaat ttttgcgcgt gtattccccc tctgccgaag tgcgcggcca
     4141 tggccacggc caagaagtgc tacaaacggg taaagaagag gtcaatatta ccaacatcga
     4201 acctgtgggt aattatgcgg tcaagcttac attctctgat gggcataata ctggcttata
     4261 ttcttgggat tatttgtacg agttagccca aaattacgaa gccctttggt tggaatattt
     4321 aggtaagtta agtgtggcag gcattcaacg tcaaagcact taaaaaaata tggaatattt
     4381 gaaatgacga cagaacacga cagtaatcag cagactcact ttggctttaa gacggtgcat
     4441 gaagccgaaa aagcccaaaa agtcggcgaa gtttttcatt cggtggcgag taaatatgac
     4501 ctcatgaatg acgtgatgtc ggcaggctta catcgcagtt ggaaacgctt tgcggtagaa
     4561 gtcagcggcg tgaaaacagg cgataaagta ttggatatcg cgggcggaag cggagattta
     4621 tctaagctat tcgctaaaaa agtgggcgtt ggtttacctc aaaacgcggg gaaagtcatt
     4681 ctcaccgata tcaacgcctc aatgttaggt gttggacgtg accgcatgct ggatgcaggg
     4741 ttgaatgtgc cggcactgca atgtgatgct gaaaaactgc cttttcctga ccagtatttt
     4801 gattgtgtga ttgttgcctt tggtttgcgt aatatgacgc ataaagaagt ggccttagcc
     4861 gaaatgcagc gcgtacttaa agtgggtggc cggctattgg tgcttgaatt ctcaaaagtg
     4921 tggcagccct tgagcaaaat ctatgatgca tattcattca agttattgcc gctcatgggc
     4981 aaaatttttg ccaaagatgc cgagagttat caataccttg ctgagtcgat acgcatgcac
     5041 cccgaccaag aaactttaaa acaaatgatg actgacgtag ggttttttaa agtagattac
     5101 tacaatcttg cagcaggcgt cgtggcgctg cacaaaggct ataaaacctg atgctgaaac
     5161 aactttccac gcgtttattg cagcacctca ttgcacaaaa tagttgggcg aatgcgctgt
     5221 tgcaccccta tgtcgggcaa tccgtgcgca tcaatattgg cttaatctcg cccacagtag
     5281 ttattttaga agatggcagt ttagccatgg cgggcgacac caacacaccc gaggcaacag
     5341 tgaccattcc gccaagcttg ttactgcgcc tgcttgccaa agatgaatca gcaaaaaacc
     5401 aaatccaaat cactggcgac actcatttag ccaccgaact tgctaaagtg tttgcccata
     5461 tgcgctggga ttatgaagac gatttaagca agatagtcgg tgatattcca gcctataaaa
     5521 tcgcagattt tggccgcagt gcagtgggtt caattaaaga caccagcact aaccttgctg
     5581 aaatgttgag cgaatattgg caggaagaaa tgccagtcat tgccaaaaaa cgccatgtgg
     5641 cgcagtttaa taccgaagtt gacacattac gtgccgatgt tgcgcgcttt gaaaaaaaat
     5701 tacgcaaact agaaaaaacg ctagccaacc ccgccaacga tcaacccgaa actcaacact
     5761 cttaattaca tgatgcgcat ttttcggctg ttctatatta tttttattgc cttacgcttt
     5821 agtctggatg acctggtact tagccacacc aagctcaaac cgctacaaag cgtgattcgt
     5881 attttattat tttggcgcaa tacaaaaaaa ccgcgtggtg agcgcttacg cctagcgctg
     5941 gaagcattgg ggccaatttt tgtaaagttt gggcaaatgc tctcaacccg tcgcgactta
     6001 ttaccgctcg acatcgctga cgaactcgca aaactgcaag accaagtgcc gcccgttgca
     6061 tttaccgaaa ttgaagccgt catacatgaa gcgtttggtt tgccgctgaa tcaagtgttt
     6121 tctgagtttg agaccaccgc tattgccagc gcatctgtgg cgcaagtgca ttttgcgcat
     6181 ttaatggacg gaacaccggt ggcggttaaa gtgttgcggc caggcattgc taagaccatt
     6241 caacatgatt tagaactact cgatacaggc gcttggttgc tgcaagcctt atccaccgag
     6301 ggaaaacgct taaagccacg tgaaattgtg gctgaatttg ccgagcatac ccacagcgaa
     6361 ctcgatttaa ccttagaggc tgccaattgc acgcggctcg cggctaattt tactgataaa
     6421 aagttactga ttcctgccgt acattgggat tggtgccgcc aacaggtgat ggtgatgcag
     6481 cgcatggtgg gcacgccgat tagtaaaatt gaagaactgc gcgccaaagg gattgatatt
     6541 gcaaaactcg cgcatgatgg tgtagaaata tttttcaccc aagtgtttcg cgatggtttt
     6601 tttcatgccg acatgcatcc tggcaatatt caggtagcaa cagacggcga atataaaggc
     6661 cgctacatcg cgcttgattt tggcattatg ggcacgctca ccgataacga taaatattac
     6721 cttgcgcgca atttcttggc attttttaac cgcgattacc gcgatgtggc cgtcgcgcat
     6781 attgaatctg gctgggtacc aaaagacacc aacgtagaag ccttagaaac cgcggtgcgc
     6841 gccatatgcg aaccaatttt caacaagccg ctaaaagaca tttcatttgg ccgcacgtta
     6901 ttaagcttat ttcaaatgtc gcgcaaattt ggcgtggtga ttcagccaca gcttgttatg
     6961 ttgcaaaaaa ctttgctgaa tattgaaggg ctaggccgcg atttagaccc caatattgac
     7021 ctgtggcaaa ccgccaaacc ttttctaaaa cgctggatga gcgagcaaat tggctggcgt
     7081 agcgttgcaa ataccgtcaa aaaagaactg ccatttattg tcaaaaatct accgcaaatg
     7141 ccgcgcttgg tgcatcaatt tttgcaacaa caaacccaag ccgagcaaga agcgccgatg
     7201 cgagcggcct tggatgccct aattaaagtg caagaaaaac aagcgcgctg gcaaaagcgg
     7261 ctgacttttg cgctcctgat tttaattgcg ctgcaaattg cttcttttgt actgcattaa
     7321 ccgcgtattt tcgccaaatt tttttacagt aaacttctat ttaaacatcg caaatttatc
     7381 gtaaaaatgc ctgactttaa ctacaaccct tttgaccttc cttgcaagcc ttgtaaataa
     7441 aggctcacag ggcagcgatt ttttctcgag cgctgtttgc catggtgtta acaagttata
     7501 atgcagtcag ttcatgcttt tatttttagt ttttcagccc aattcacttg tgcacttgaa
     7561 agatgattcg atgatacaaa aaaaaagaat aattcaattc gcgctgctca gtgccaacat
     7621 gctgatgcta aacgcacacg caactgaaat tttagtccct gcgtactttt atccctcgag
     7681 cgacccaaat ttaagttttt gggatgaaat gacggctgca gccagccaag gtgttggcat
     7741 tactgccatt atgaacccca atagcgggcc gagtactgcg gaaaatagcg actacaccaa
     7801 cgctgtaagc acttttcgtg ctgcaggtgg caaagtgatt ggctacgtgc cgacagggta
     7861 tggtgcgcgt gatgcgagcc tagtgctggc cgatgtgagc gcctataaaa gtttttatca
     7921 gattgacggc atttttttag atgaaatgca caacattgca agtgaactta cctattatga
     7981 aagcttgcga acaggcattt caggcattga tgtcaactac aaagtgtttg gcaacgctgg
     8041 cacgaatacg ctagaagact atatgcatac ggctgatgtg ctggtcacgt ttgaaaatca
     8101 aacaggctac accgcatacg cgcccgattc ttggacaggc aactacacag ccaatcgctt
     8161 tgcgcacttg ctttataacg ttggcacggc ggctgacatg caagcggcta ttacgctcgc
     8221 cgcaagccgc aatgtgggct acatttacgt gaccaatgac aatggcgcta acccgtggga
     8281 taccctaccc agctattgga atgccgaagt taccggtgtt tctgctgtgc cagagcctga
     8341 aacttatagc atgatgttgc tgggtttggg cttaatcggc tggcaatata agcgcagaaa
     8401 tgcactaaaa acatccgatt aactttaacg cttttggcaa tgcgcgcaaa aaaacgtaga
     8461 gcgttgccct aaacgaatgc actgaatggg ttttgcgcag actttacaag gttcatcggt
     8521 gcggccgtaa acaaaatatt cttgctgaaa ataaccgggg ttgccatcgg tgccaaaaaa
     8581 atctcgcagg ctgctgccac ccgcattcag cgcatcctgc aaggtgtttt tgatttctgt
     8641 cactaagcgt tcacactgtt ttaaactcaa attttttgct ggcgtttcag ggtgaatgcg
     8701 cgcgcgaaat aacgattcac tggcataaat attgccaacg cccactacca cgtggccatc
     8761 cattacggca ttttttattg caagcgattt actgctaagg gtttgatgca gatatttggc
     8821 attaaagctg gcctccaacg gttctgggcc aagctgattc agcaacgcat ggtcatgatt
     8881 ttgattatcc aaccacagca ctgcgccaaa acgacgcggg tcgcgcagcc gcaatacctg
     8941 tgcatcgcta aattgaatat caaaatggtc gtgtttttct ggcggataat ggcggtctag
     9001 tagctgaatg cggccagaca tgcccaaatg tagtaacagc gtacccgtat caaacgcgca
     9061 caaaatatat tttgcacggc gggtaagttt gtgcaaggtt tgatgtggca gtgtttgcgt
     9121 taaatgttgc ggaatcggcc aacgcaaggt tgggtggcgt atttccaccg cttgtaccac
     9181 tttgcccagc agtggcaata agcccaagcg cgtggtttca acctctggca gttctggcat
     9241 tggccgctat ttgccgactt aattactagg caagttaatt ggcgggttta gcatcgtaaa
     9301 gccaatatcc gattgaaaat tgtaaatcag tccttcatca gggcgcgcca atactaaatc
     9361 aggtgactct tgcagtttat caaaatgcac tctgctacca tcagccaatt taatttccaa
     9421 tgacggatat tttgcttttg ggtctggcgt ataaaactca acagatttcg ccgcagtttt
     9481 cacccatgaa aactctagcc actcatttaa ctcatcctgt tgcggttttg ctgctggaat
     9541 ggaaactttc catttaccct ccaccaagtc cacattgagc tttgcttctt cccattgctc
     9601 taggcgactc aagtcaaaac cagccacttt ttcagtgggc tttaatggcg ctttatcaat
     9661 gagttcaatg acttgcgtgg tggccgcctc ggcataggca ttgtcaatca agtagactgc
     9721 attttcaagc agcacgtatt gcgcatcgtt aatcgggtta tgggtaccaa aaataaagcg
     9781 tgccactgat tggtcagcac gaatcaactt taattgtaat ttagggttgg ttaagccaaa
     9841 tttttctaaa ctggcggtgt cggcactgtc taccagcact ttttcttttg atcttgctgc
     9901 aataatcgac aaaatacgcg ccactgcatt ttggtcagcg cgcgctttgg tgggggctgt
     9961 taagcgccaa aaaccatcca ctttagcaaa cgtcactggt gctttggtag gcggttctac
    10021 actgatggca gtaaattctg ccaacttgta attagagagt tcatagactt ccgcttgttg
    10081 cacgttttcc ttggggcgca gatataaaaa tgccaccaaa ctagcgaccg cacacaacaa
    10141 taccaaattc aataaccaac gttttttcat gcttgacctt taatctaaat aaccaaaatg
    10201 aattgactta aaaaagttaa attgagccgc taaaactctc gagaaatatt ttctgccctg
    10261 cgagccttta tttataaagc tcgcagggca tcgaaaaagg ttgtgctgta aaacagcgta
    10321 tttcaacatg caatttagac ctattgaatg ttgaatttta attaggcttt tctacgtttc
    10381 caccagaagt aaaatccagc aatcatcatg ccgatgggga aaaagaattg aaagccatga
    10441 aacacggtcc acgccaccaa gcgacctgta tcagaatcag gaatcgtcac attgatgtct
    10501 tttaacggca ttggctgaat ggtaataagg ttgtcatcgc ctgccagcca gttaacaata
    10561 ttcacgccta aatcgaggtt gccgccattg gtaataaagg tgttagataa aaagtttgcg
    10621 ttccccatca ccaccacgcg ttggcctttt ttgccgtaaa tacgctcaag ggctacgccg
    10681 atattgatgg gcccgcgctt gtcagttttt tcattgaatt ttggtttggc gtctttagcc
    10741 aatttatctg ccgtaagcca gccattgggc gccacttcta ccaaattgct gactttccag
    10801 ccattttcat ctgtgccttt ggctgtgacc tgatgcgcct caggaaacaa agtccgcaac
    10861 ataaaattag tggtaatcgg atgctcgcca tacaaactgg caaacgccac acgcgcatct
    10921 gcaccatatt gggcagaggc catatccagc gctatgccag gggatacttg catgcccaaa
    10981 tattctgcca cttggtctaa accgcgcaaa ttattgtcgt ccagcaacca aagcaaattg
    11041 ccgccgcttt caagataggt cttgattttt ttggcttcaa tttctgatac atctacttgc
    11101 gggctggcaa tcaccagcat cgcaccgtta cttggcacgg cttgggcaat ggtcaaatct
    11161 gggttggcaa atttaaagcc ttttttctcg agctgcttac caaattcacc aagatcatga
    11221 tttctaacac ccagcaaatt acgctcgcca tggccatcta aatacatcac cgcttgctgg
    11281 tttgtgcgtg ataagcgcac cagtaaattg gtcatttcct gctctgcaat cggcgggtta
    11341 atgtgttcaa cacgtttttg gtattccacc accacttcgc cgtcctcttt tacgccagcc
    11401 tcttgcgcca gttttggctc aattgacggg ttaataaaag tgaggttcac atcttttttc
    11461 tcacgctgat agcgcgccac aaaatcaatc atgcctttac gaaagttatc gccactcgag
    11521 gcatcatctt tggtagcaaa caccgtgata ttaatcgggc ctttcatttg cttaagcaca
    11581 ttcacgctac cctgcgtcaa aatgttgcgg ttagcttgtg tgatgtcttt tgccacatga
    11641 tactggcttg ccagataacc cagcaaaatc accaacagca aaaatagcac tacaaaaaaa
    11701 ccgttttgca caaaaagttg aaaacgtaat ttacgattta tattcatgtt tacaaagacc
    11761 taatatcgaa caacttaaag atgaaaatct aaaaaaatac cctattaacc gcgtaaacgg
    11821 tcagcatcta aacgacgcac tgtcagcgtt aaaaatgtgg caataaataa gacgaaatag
    11881 atgatatctg cggtatcaat caaaccactt gcaaaggact gaaaatggcg tgtgagcgac
    11941 aaacttgcaa gcgtattact tgggtcaccc gcaaaaaagc gatctaaaaa catcaacgca
    12001 aacaaggcaa taaacgttaa aattgccgca actacgggtt gttgcgtcat gcttgaaaaa
    12061 taaagcccaa gtgccgaaaa gcttgccacc atcagcagca tgccgagtga gtttgccaaa
    12121 atgtagccaa aatcaatatc cgcccaaatg ttcagagttg agagcatcaa gccgatgtat
    12181 acaatcaaaa tacttaaaaa taaaacaagg cctaaaaatt taccaatcac aatttcagtc
    12241 aatgaaattg gcgcgctgaa caaaaacggc agcgtttgat tacggcgctc ttctgaaatt
    12301 aaacgcattg aaagtaacgg caccgcaaac aacacaataa atgaagcaaa gccataaacg
    12361 gtttgcccaa caaactgcgt gacacctgtt cgctctgcgg ggcgcatcgc gcctgacatg
    12421 accgcaaagt aatcattcac cccattgaga taatatgttc caaacgcaaa catcaggagt
    12481 gataaaatca cccaacccat gggggatgag aacatgcttt tgagttcttt tcttgctaca
    12541 ttaataatca tgattcatgc cttaaattta ttttatgcca agtttcttgt tgcttaggct
    12601 gcttgttctt ggtaggtgag ttgtacaaac acatcttcca aactggtttg gtctggcgca
    12661 atttggtaca aaccccaacc attactcacc gccgcctgca caatggattc agccggtgcg
    12721 gtgtcatcgg caaagcgcac gcgcatttgg ccgtttggca aactctctgc ctcaaccaca
    12781 ttaggaatac tcaacattgc ctctacagtt ggcggattac gcatcgcaat cagcaactta
    12841 ttaccaatgc gttgtttttt cagcacctca atcgcgccgt taaacaccag cttaccttta
    12901 tcaataatct gcacatggtc acaaaccatt tcaacttctg gcaaaatatg cgtagataaa
    12961 atgacgctat ggtccgagcc aatttcacgt atcagtgcac gaatatcacg gatttgaatg
    13021 gggtctaagc ctacggttgg ttcatctaaa ataaccacca tcggcttgtg aataatagac
    13081 tgcgcaatgc caacgcgctg ctgataaccg ttggataagt tttcaatcag gcgcttactc
    13141 atgtggctta aaccacaacg ttctttggct tcttcaacag caggcttaat gtgcttggca
    13201 ctcacgcgat gaagccgcgc agcgattgtg aggtattcat ctacggtcaa ttctttgtag
    13261 agtgggcgca tttctggcaa atatccaatt aaagctttcg cctcttttgg gttttcgatc
    13321 atatcaatgc cacagatttt tacactgccc tgactaggtg caaggttgcc tgtcagcatt
    13381 ttcatggtgg tggattttcc tgcaccattt gggcctaaaa aacccaacac ctggccttta
    13441 cttagggtaa aactgacatt gctaaccgct tcgcgtccac cgtaaagcct ggtgagttca
    13501 attgcttcta ctgtatagtt attcatattt actcaatgtt taattgctca aagatttcat
    13561 gacacaaaaa ataagtgtcc atttgtgtgt cagcgctggc atgaagttca catgtcgcac
    13621 ataaattcat ttgacaccgt tcttagtcaa ttcataatta tggaggaact tgagacgact
    13681 gtcacggact aagcactcga gccatgcaat aatactgttg aattagccat ttaaaagtca
    13741 tgcaaacagc tttaaatgca ctaagaacgg tttttaactc accactttaa tcatacggaa
    13801 cttataatac tactttattt cgtcactaga atttaagttc cacaacggta cataaagcga
    13861 ctcgcctttt aactttgaga aatcaccatc atcgctatgc gcaaatatat tggtcttcaa
    13921 acaaataaaa ctaaaacaac ttcgcgctat accaaagctt tttacgcagg cctgattgta
    13981 agcacaatta gccttagcgg ctgcgctaca aacaaacctg tgagcgatac gttagcaagc
    14041 agcaacaagc cacaaccaac gcaaaccggt ttatctgcag agtttgtcta caagtattta
    14101 gtggcagaga ttgctggaca gcgtggcgat tttgcaacat caggcgcagc tttttacgac
    14161 cttgccagaa cctctagaga tccaagcctt gcagaacgcg cagcaaaaat tgcggcttac
    14221 ggcaaagtgg gcggcttggc aattccagcc gtaaagctct gggcagagtt agaccctagc
    14281 tctaatgaag cgcagcaggc catgacagaa ctctacattg cgacgggcaa gctcaatgaa
    14341 gccaaaccgt ttttagctca gcttttagtg aaagaagaaa cacgtgcggg tggattttta
    14401 tacctgaata cattgctagg caaaagtcca gatagaaccg ctgcgctaaa actagtgcaa
    14461 tctttggctg agccatatcc agaacttgct gaagcacagg tggccattgc tcaggcggct
    14521 tgggcggcaa aacaaaatga cgcaaccctc aacgccttaa caaaagctga aacacttaaa
    14581 ccaggctgga acatccccgc cctgttaaaa gggcaagtgt tatttgagca atctcctgaa
    14641 gccgccatca agttttatca agatttttta gtaaaatatc cgcaatcaaa cgaagtgcgc
    14701 ctgaacttgg caaaaatact cgtaaaccaa aaacaatatg atgctgctaa gaaacactac
    14761 cctattatta ttcagcaggc aaaagaagag cctgaaaaaa acacggctga agtcaccgcg
    14821 ttaattggat tgctctcatc tcaatctggc gactatgcgg cggctgaaag ctactacaag
    14881 caatccattg agctaggctt taaaaaccct gaccaactgc acatttatct tgcgcaagcg
    14941 gccgaaaaac aaaagcaaga caagtctgcc attgattggt ataacaaaat tcagccggca
    15001 agcgcgcatt atcttgaggc acagcttaat cttgccaata ttatcgcccg caccgagtca
    15061 gccgataagg caattgaaaa attagacacg attgacgact taacgaccga gcagcaaatc
    15121 atcgtaattc aatctcaggc tagtattttg gcaaaagcaa aacgcgacca agaagctttt
    15181 gatttgcttg ataaagcggt aaaaaatatg cctaacacct ctgagctggt gtatgactac
    15241 gcattggccg ccgagcgcat taaaaagttt gacctcatgg aagccgagct gcgtaaatct
    15301 attgccgtta aacctgactt tgctgccgcc tacaatgcgc ttggttattc attcgcagac
    15361 cgcaacattc gccttaatga agcaatcaaa cttatcgaaa aagctttatc ttttagcccc
    15421 aatgatcact acatgctaga cagcctaggc tgggcacact accgcaaggg caacttagac
    15481 aaagcaataa cgtatttaca acaagcctac aacaccagcc aagaccctga aattgcggca
    15541 cacttgggcg aagtattatg gaaaaaaggc aaacgcgatg aagccaacaa aatatggagt
    15601 gacgcgctca ccgccaatcc agaaaacgaa gtgctgatta acaccaccaa taaattcaaa
    15661 acgattcaat caaactctta gcaaaagcaa atggttagca ttcaatcaac tcaatggcag
    15721 caatcaattc ggattgcgct agtgattgga ttgatggcct ttttgcaatc ctgcgtgagt
    15781 gtcccagcaa tcaaacccgc tgagagcgcg gcttcaaaag ccttgtattt aaagcatcag
    15841 caagagattg cagcgattca gcaattttcg ctacaaggcc gcattggtgt tcaatctgaa
    15901 aataaaggtt tttctggcgg cctgacttgg cagcacgata atgcaaacga tgacatctca
    15961 cttttctcac cactgggtgg gcaagttgcc agtatcaaaa aaaccgccac gcaagtgacc
    16021 ttggtagacg acaaaggtaa cagcatttct gaagtagatg ctgaaaccct gacacaaaaa
    16081 accttaggct ggcaattacc gctttctgga ctagccgact gggcgctagg aaggccgtca
    16141 agcaaccaca taatgaatag cacatgggat gagcaaggtt atttaattac attaaaacaa
    16201 gatggatggg atataaagta cgaaaactac tctgaacaaa atggtcgctt tttacctaga
    16261 aaaatattgc taaaaaatga aaaagtaagc ctgaaactgc taatagaaga atggaattag
    16321 ttcgaaaccc gtggtttata atgtagagct tgtagaaaga gttgacgtgg aaaataaaag
    16381 gcttataatc aatactaatt atttttgaaa ttaggggtac tatattatga aaaaactttc
    16441 caagtactta tcagtcgggc ttttggttgc ggctagcgct gcttcattta ctgtaaatgc
    16501 tgcagtagtc aacttaggaa atttgtctgg cacttatgca aacacgtttg aaagaagttt
    16561 tggacctttc atagacaaca ttaaattcaa aattacgacc ccatcgaatt tgggttcagc
    16621 ggcaaatcac cttgatttaa gttttaacat acctgggata ggtaatgttg acttgtacaa
    16681 cattactggg atgtattaca acattacagg tccaggtagt tttactacag gtaccttatc
    16741 agcagacaac actactcact caacaatatt aaatgttgct ggtgattatc aagttaactt
    16801 atttggcaat gcaaccggga caaaaggcgg gttgtatgca gtagctttaa acgtaacagc
    16861 agtgccagaa cccgaaactt acgcgttgat gttagctggc cttggtttag taggttttgc
    16921 agcacgtcgt agaaaatcag ctgtttaaac tttagtaaaa gttattaaaa aaaggggctt
    16981 aacgcccctt tttgcaattt atgagcaacc tattataagt ggatgccact tgtagccaac
    17041 tttcactttt taccttcttt agctaaacat tgaacttagt tatccccttt cacgcattta
    17101 cgctaaaatg gcgcattgtg acttgctcgt taaatctctc taaattttga acaccccaaa
    17161 aaaagtcttt attaaaacct ttggttgcca gatgaatgaa tatgattcat ctcgcatggc
    17221 ggatatgctg agtgctgaaa atggcatggt agaaacgctc acgcctgaag atgccgatgt
    17281 gattttgctc aacacctgtt cggtgcgcga gaaagcggaa gataaagtat ttagccacct
    17341 tgggcgcttt attccattga aaaaagccaa ccctaatttg gtgattggcg taggtggctg
    17401 tgtagcttcg caagaaggcg acaacatcat taagcgcgcg ccttatgtgg atgtggtatt
    17461 tggcccgcaa accttgcacc gcttgcctga gatgattaaa agtagccaaa gtagcggtca
    17521 atcacaagtg gatatttctt ttcctgaaat tgaaaagttt gaccatttgc cgccgccgcg
    17581 cgttgaaggc gcgagtgcat ttttgagcat tatggaaggc tgtagtaaat attgcagctt
    17641 ttgcgtagtg ccatacacgc gcggtgaaga agtctctcgc ccgtttgagg ccatattaac
    17701 tgaagccatt caactggcag aacagggcgt gaaagaactc acgttactgg gccaaaacgt
    17761 gaatgcctat cgcgcagaat atcaagggac tgaggctgat ttggccttac tcattgaata
    17821 cattgctgaa attccgcaat tagaacgcat tcgctttacc accagccatc caaatgaaat
    17881 gactgagcgc ttaattgctt gttttgccaa cattccaaaa ctcgcagcgc aattacactt
    17941 gcctgtgcag gcgggggctg accgcatatt gatggcgatg aaacgtaatt acacagcgct
    18001 acaatttaaa tcaaccattc gtaaactgcg cgcggccaac ccaaatctca cgtttacctc
    18061 agatttcatt gtcggttttc ctggtgaaac tgaagctgaa tttgaagcca ccgcaaaact
    18121 catgcaagac gtgagctttg attatagctt tagcttttta tacagccctc gccccggtac
    18181 ccctgcatct tatttaactg atgaaacacc acaatcaacc aaactagcgc gcctagctaa
    18241 gttacaagca ctgaacgaag cacaaggcaa actgattagt gaagccatgc ttggcacggc
    18301 gcaacgcgta ctcattgaga gcgcttcttg gaaaaatgcg agcgaacttg ccggcaaaac
    18361 tgataacaac cgcattgtag atattgtggg cgatgcaaat ttaatcgggc atgttgtgaa
    18421 cgtacgcatt actgatgtat cgaacccaaa acgcctgcgt ggtgaaatca ttaacggata
    18481 acctattgaa gcatatggaa attaccctga ccccagaaga taac
//
LOCUS       DAOQSI010000021        18116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_2199_length_18116_cov_5.819279, whole genome shotgun sequence.
ACCESSION   DAOQSI010000021 DAOQSI010000000
VERSION     DAOQSI010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 18116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18116
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2199_length_18116_cov_5.819279"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="PKL42_02700"
     CDS             complement(<1..552)
                     /locus_tag="PKL42_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNU66010.1"
                     /translation="MNLHEYQAKTLLQKYNIPVPRGQVISVAKQTSAVTSEIDSQAWV
                     VKAQVHAGGRGKAGGVKVVKSPKEAQTVASELLGKTLVTYQNTPDGQPVNQVLIEETL
                     PIARELYLSMLVDRTLERVVVVASVAGGMDIEEIAHTSPEKILQEVCDPLNGLVDFQA
                     RNLAFKLALSGEQIGAFSKILKGL"
     gene            655..1257
                     /locus_tag="PKL42_02705"
     CDS             655..1257
                     /locus_tag="PKL42_02705"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HNU66011.1"
                     /translation="MTTPSPSLRDITGMGYEQPGLSNSALIMVDCQNTYRQGIMQLTN
                     VEPAIKEAQKLLQLARDLKIPVIHIQHDAGVSTPYDVSAEIGAISKEVAPIKGENVII
                     KNYPNSFVSTPLEAQLKAIGIKNLVIAGFMTHMCINSTARGAFNLGFSPVVVASACAS
                     RNLTGANGKVIDAQTMHDSALAALRDLFAVIVDDVASLKS"
     gene            1323..2162
                     /gene="serB"
                     /locus_tag="PKL42_02710"
     CDS             1323..2162
                     /gene="serB"
                     /locus_tag="PKL42_02710"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HNU66012.1"
                     /translation="MRLVVQGRAVSVAHLTHIHRLIGGSTQFMQIAEHAYYLPTNESN
                     IEPVLQFCAEQHIDCALVPDAQRLSHIGLAVMDMDSTLISIECIDEIADMMGIKPQIA
                     EITEAAMRGELDFAASLRKRVSLLKGLPESALQRVIDERLQLNPGAREWIAACKANNI
                     KTMVVSGGFTLFANHTQSLLGLDYAVANTLEIIDGKLTGHILGDIVDASRKAHELEKL
                     REALGLNVSQTIAIGDGANDLKMMSTAGVGVAYHAKPVVQAQATYALNFVGLDGIANL
                     FTA"
     gene            complement(2376..3155)
                     /locus_tag="PKL42_02715"
     CDS             complement(2376..3155)
                     /locus_tag="PKL42_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2135 domain-containing protein"
                     /protein_id="HNU66013.1"
                     /translation="MILLVLSALHVQIAAADTANTLESPRGGWNYSGMTDRAGEARVA
                     YPTPPIDRGAQRNRTLIKGRIAAAGTNRTPKLVVNGNPLPLNVGAEGRYERHYAFGSG
                     SNSVELKSPNGTSVRRVQFYESNPTKQLPKLRIICAWDAPEAEVDLHVLTPDGQHVTW
                     SSPILKTGGGLDVDSVDGPGPEMFSTSTPIRGAYNIYINYWGNLNEAGYNFDQSQLQK
                     PIITTRITLVFNENTPNEKREDFVVPLRKIGELNFIKSFVY"
     gene            3412..4587
                     /locus_tag="PKL42_02720"
     CDS             3412..4587
                     /locus_tag="PKL42_02720"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNU66014.1"
                     /translation="MKKRLQFLVQRIQALRAEHDKVAQAALQDTLQRQPLLLGLAIFS
                     MLLGAKVNWQSIGALAPTALQGIHHASSLNLIMAVWLISAGLFLNWGRSKYCNTTALL
                     ALATSFLIFGVLRAAIDQWGSGSVNMFSLCCVFTGTLILIRPAHAIALYAGSYLLFYI
                     AMALTQKNPVILAINQAQGFAAAALGLLLAFLLWRKHTVTTLLQQQLQASNEELQRQK
                     QQLEILSQLDALTGLYNRREFDRLANIELVRAKREGSDTAIIMADLDFFKRINDCFGH
                     PTGDEVIQKTAEALSQNIRASDIVARIGGEEFIILLPKTSLDEALVVAEKLRLSLQEN
                     AIQTQISQTIQMTASFGVTAMATHQQGSLNALYAAADLALYAAKNKGRNRVESQNIS"
     gene            5047..5460
                     /locus_tag="PKL42_02725"
     CDS             5047..5460
                     /locus_tag="PKL42_02725"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNU66015.1"
                     /translation="MSNLSTAVGAERRRYERFPFRRMVTLNFYEGAPIELPAIDISAG
                     GIGVRSHINIATDKMCMANISIVILQGNLSFEASAQVKHSIFSRSENGFILGLNYVKI
                     EPSLQTAIVCLMRGLSEQINTTHDAIARLGRHHTL"
     gene            complement(5467..7146)
                     /gene="recN"
                     /locus_tag="PKL42_02730"
     CDS             complement(5467..7146)
                     /gene="recN"
                     /locus_tag="PKL42_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNU66016.1"
                     /translation="MLQALSIRDFVIVDQLNLEFERGYTVLTGETGAGKSILIDALSL
                     ALGARNEGDVVRAGCDKAEMTAHFSLHDNLDAQAWLNANEMQTDENELVLRRVIYSDG
                     RSRAFINGIGATVAQLKEIGEYLVDIYSQNAHHSLLKLATQRNVLDDFAGASDLAQAV
                     AESFKVWHQLHRTRLEMEQNASKYADELAELRDKTRELSQLGLATDGWKDEWENLQQE
                     HSRLSHGASLLSGCEACRALLSEGEISALRQLATTQHKLQMLRDFDAQLDEPLASLDS
                     AIIQLEEVDRFLNRYVQKTELEPAQLAEIEARIQALHAAARKYRSTVDMLPTLLAQWQ
                     ARMAELASVGDDGELLKKEAAALATYHVHAEKLSTGRQKAAQQLSEKVTAEMQRLSLA
                     GGKFEVLLTPQEKSATGLEQVEFLVAGHAGVAPKPLNKAASGGELSRISLAIRVVTAQ
                     QSSVDRHSMNSMIFDEVDVGIGGGVAEVVGQLLKQLGQARQVLVITHLPQVAALGSQH
                     LRVSKSLVNGQTLSTIVTLNQTSRVEEIARMLGGLELTETTRQHAQEMLAR"
     gene            complement(7209..8072)
                     /locus_tag="PKL42_02735"
     CDS             complement(7209..8072)
                     /locus_tag="PKL42_02735"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HNU66017.1"
                     /translation="MNTPFNKIALIGKYQDREMREHIVALAEFLRARHLHVFLETETA
                     RCCEIADYPIRALTEFNGDIDLAIVLGGDGTMLGVARALLEHQVPLLGINRGRIGFLT
                     DISAQNMLEDVAKVLAGDYSIEQRLMLHTKILRKGKLLGEGVALNDVVISKGEVAQLL
                     GLEVMIDEQFVHRQRSDGLIVATPTGTTAYALSAGGPILHSALEAITLVPICPHTLSN
                     RPIVIKSTSQVHVKLVDQYESRIHFDGQLIARLYQYDDVYIARAEKMTRLLHPNGHCH
                     YEMLRKKLHWG"
     gene            8415..10304
                     /locus_tag="PKL42_02740"
     CDS             8415..10304
                     /locus_tag="PKL42_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNU66018.1"
                     /translation="MPSTTQKKILTLYFAMTYFLALWLVSDSISFSSAFEQSWLTLGF
                     MLLAFLLYGLYYLLPSILITALMGQFTRPNNSRPVYITAIITGAITTLLLYANAKIFA
                     LYGMFFNGFILNLVMSPGGIESLGGSTASDVGYALIALGFVLVQILILWLAKRFYQYK
                     TQTVLSFKWLPITAFVATILVHMGFALDSYTTTQLNVVAESVPFYQTVSARSFFSSLG
                     FKTQRDTKLKVKGKLNYPLNPIHFQQPAKPYNIIWLTSESLRADMLDEKIMPNSWAFA
                     KNAARFTRNYSTGNGTRMGVYGMFTGTPGNYWFSFLESRRGASIIDVLQQQHYQMSFY
                     TSAKFSYPEFDKSIFARVPTDQLHEIEKPKSGWESDRDNVSHLLAFIDQRNTTKPFFT
                     FMFFESPHARYYFPPESVIAKPYRDDLNYATLSKEQLRNDIVPIKNRYINSVHHLDAQ
                     FGRIYDYLRQHQLLENTIVIMNGDHGEEFMEKSFWGHNSTFVDEQVKTPLVIYKPGMQ
                     PLVSNQMTSHMDVIPTIMPLLGVTNPSSDYATGYNLLAGEKRTHTYISDWDKVAYVDD
                     EVKITQPVNGKSFALVKASKGDDSALTPEERKAVLAKKQPAMLQLVNDLSKFFKKKET
                     SPQPQ"
     gene            10355..11365
                     /gene="hrcA"
                     /locus_tag="PKL42_02745"
     CDS             10355..11365
                     /gene="hrcA"
                     /locus_tag="PKL42_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HNU66019.1"
                     /translation="MDKRAQILLKTLVEHYISDGQPIGSRTLLQHSGLDVSAATIRNV
                     MSDLEQLGFITSPHTSAGRIPTQKGYRLFVDSLLTVQPLENKTVQQLKSGLTSPNPTE
                     LIANAADMLSQLSHFAGLVVTPKRKRIAFKHLEFLALSEKRILVIIVTSDNNVQNRII
                     LSEKPFTASELTQAGNYFNAHYSGYTFEEVQQKLHIELKQMQTDMNKLMSAALEASNQ
                     TDADDGVVIAGERNLLHVDELSTNVASLRKLFDIFERRTSLMQLLDNSQRAEGIQIFI
                     GGESGYLPLDECSMVTAPYEADGQIVGTLGVIGPTRMAYERVIPIVDVTAKLLSNALS
                     TN"
     gene            11386..12501
                     /gene="hemH"
                     /locus_tag="PKL42_02750"
     CDS             11386..12501
                     /gene="hemH"
                     /locus_tag="PKL42_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008106758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNU66020.1"
                     /translation="MPFYNKEPQYEHGDQLKVGILLANLGTPDAPTAQALRPYLRQFL
                     SDRRIVEIPRVIWWFILNCIILVVRPKKSAEKYAQVWTKEGSPLLVHAQKQTQLLRGY
                     LSQKINSPHVVELGMSYGNPSMASAIEKLKAAHCDRILVFPLYPQYAASSTASALDAV
                     WRTLLKMRNVPAIRSIRSYHDHPAYIAALAKSVQEHWVIHHKPDFSSGEKLVMSFHGV
                     PKVHLTSGDPYHCQCHKTARLLAEALNLNKTDYIVAFQSRFGKQEWLKPYLASTLEDL
                     GKAKTKRLDVICPGFSSDCLETLEEIAMEGKHIFQSNGGGEYNYIPALNENDAWIQAM
                     TTIALENLQGWVRADWDSEAAKLQNTRTKNNAKLLQP"
     gene            12805..14268
                     /locus_tag="PKL42_02755"
     CDS             12805..14268
                     /locus_tag="PKL42_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNU66021.1"
                     /translation="MSLNACTALKNLSQQHSPQNTINTTKVADRWQATLPHGGNSANL
                     AQFWQQYQDPLLLKLIEAAQKESSSIATANSRIAQARATRTQANAALTPTVDGSVSAS
                     RSVQQPATSIGGSQQGNIGGGSGAFNNAQISAQASWELDIFGVNRGAFEASKAQENAT
                     LASWHEARVSVAAETANAYFNQRFCSTQANVLEADVISRTETLRLTNIAVKAGFSAPA
                     NSHLAEASLADARQQRTAQMAQCDLDVKALVALTAIDEATLREKLNQQDFVRLHESTN
                     NLFTLNEIPAQVIAQRPDIYAAEADLVSAAADIQTTYASSLPKVSLNGSIGWMWLSGS
                     GFSTNGKTWSLGPISITFPIYHPGVKEATLASSEAKYEEKASIYRSKVRTAVKEVEDA
                     LVNLHSANSRQADIAQAIQSYQASFDAMQTKVNAGFANLIDLEDQRRVLLNARTNAIN
                     NQKQQAVAWVSLYRAAGGGWDKTEKFKTEQLETGSTP"
     gene            14265..15368
                     /locus_tag="PKL42_02760"
     CDS             14265..15368
                     /locus_tag="PKL42_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNU66022.1"
                     /translation="MISKTLIPLLIAASLLLGGCQSEQDSSPETTTSKEAVAKASLTV
                     STIKPQQTALNKTLNANGNIAAWQESSISTETNGLLLREVLVGIGDQVKRGQVLARFA
                     AASIEAEVAQTQANVAEAQATLIEAAGNANRARSIQDSGALSRQQIEQYITAEASAKA
                     RLDAAQANLRAQLIKIKQATVTAPDDGIISQNPATVGTVYAAGQELFRLIRKGRIEWR
                     AELTSSDVSFIKAGMQAQITLPDGATAQGKVRTIAPSVDNKTRNAIVYVDIPAGSAKI
                     GMFARGQFLLGNTDSLTLPNDAIVMRDGFAYVMQVHNNKITQTKVQLGQRAGDQVELL
                     NFNAANAEIVASGGAFLTDGDRVRVVNKSVGNQ"
     gene            15365..>18116
                     /locus_tag="PKL42_02765"
     CDS             15365..>18116
                     /locus_tag="PKL42_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNU66023.1"
                     /translation="MNISSWSIKNPVPALILFILLTLAGSMSFNSMKVQNFPDLDLPT
                     VTVNASLPGASPDQLETQVARKIENAVAKLQGLRHIYTTIQDGTVRITAEFRLEKPVQ
                     EAVDDVRSAVSEVRADLPSDLREPIVSKLNIASSPILAFTIHSKRMDDEALSWFLDDA
                     LTKKLLGVKGVGNVARVGGVNRQIWVELDTAKLQSLGTTAAEISRNLGRIQLEVAGGT
                     TKLSGSEQPIRTLANVKSADELAQIEIALNDGRKIRLNQVANVLDTIAEPKSAAFLNG
                     QSVIGFEVSRSKGASELEVGAGVYDALKAIQLQYPDIEITEAFNFVQPVQDEFDASMT
                     MLYEGALLAVLVVWLFLRDFRATFVSAVALPLSVIPAFIGMKYMGFSVNIVTLLALSL
                     VVGVLVDDAIVEVENIVRHSRMGKPPFEAALIATEEIGLAVVATTFTLIAVFLPTAFM
                     DGIPGKFFKQFGWTAAFAIFASLLVARLLTPMMAAYLMRPSKVETETDGVVMRTYMRL
                     ARWCVQHRLMTTVAAILFFIGSLSLIPLLPKGFVPADDNSQTQVRIELAPGATLAQTI
                     AISEQARRILKDVAHVNSVYTAVGGGAAGSDPFAGGGTAETRKATLTIQLDPRGKRPK
                     KQVIEAEIREKLQALAGVRLGVGLEGSGEKYVLALTSEDATALQNAASAVEKDLRKIQ
                     GLGNIASSAALVRPEIIVKPDFAKTADFGVTSAAIGETLRIATIGDYEQFLPKLNLEQ
                     RQIPILVKLNNDARQDLNILGKLAVPSSNGSVALEQVASLQMSSGPAVISRYDRSRNI
                     QFNIELSGLAMGDAVEAVKNLPSIQHLPAGVMQTELGDAEMMGELFSSFGIAMLTGVV
                     CIYFVLVLLFKDFLQPITILVALPLSLGGAFLGLLVTGKALSMPSMIGLVMLMGIATK
                     NSI"
BASE COUNT         5035 a         4418 c         3949 g         4714 t
ORIGIN      
        1 caggcctttg aggattttac tgaacgcccc aatttgctcg ccacttaacg ccaacttaaa
       61 cgctaaattt cgtgcttgaa agtcaaccaa accgttgagc ggatcgcaca cttcttgcag
      121 aattttttct gggctggtgt gcgcgatttc ttcaatatcc attccgcctg cgacagatgc
      181 caccactacc acgcgctcta aagttctatc caccagcatc gacaaataca attcgcgcgc
      241 aatcggcaac gtttcttcaa tcaacacttg gtttacaggt tggccatcgg gcgtattttg
      301 atacgtcacc aatgttttgc cgagcaactc actcgcaact gtttgcgcct cttttggcga
      361 tttaacaact tttacgccac ccgccttacc gcgcccaccc gcatgcactt gggcttttac
      421 cacccacgct tggctatcaa tttcagaagt caccgctgaa gtttgtttag caactgaaat
      481 cacttgccct cgcggaacag gaatgttgta tttttgcagt aaagttttgg cttgatattc
      541 gtgtaaattc attttaaagt gcttgttgaa ggtaattatt ttctaggtag tattttggcg
      601 caaaagcgaa cgataagcta gtaaaaccct aactaaaaga aaggaagttg cttcatgaca
      661 accccctcac catcattacg tgacattacc ggaatgggct atgaacagcc gggcctttca
      721 aactctgctt taatcatggt ggattgccaa aacacttacc gccaaggcat catgcaactc
      781 accaacgttg agcctgcaat caaagaggca caaaaattac tacagttggc acgtgattta
      841 aaaatccctg ttattcatat tcaacatgat gctggtgtta gcacgcctta cgacgtatcc
      901 gctgaaattg gcgcaatttc aaaagaagtt gcgccaatta agggtgaaaa tgtcatcatc
      961 aaaaactacc ctaattcgtt tgtttctacc ccacttgaag cacaattaaa agcaatcggc
     1021 attaaaaatt tagtcattgc agggtttatg acgcatatgt gcatcaactc caccgcccgt
     1081 ggcgcgttta acttgggctt tagtcctgtg gtggttgcca gtgcctgtgc gagtagaaat
     1141 ttaacggggg ctaatggcaa agtgatagac gcacaaacca tgcacgacag cgcactagct
     1201 gcactacgtg atttatttgc tgtgatagta gacgatgttg caagcttaaa aagctaaatt
     1261 tgtcatctag cccctagcca agaaaagcta taattctagt ttaacattgt tagggctatt
     1321 tcatgcgctt agttgttcaa ggtcgcgccg ttagcgtggc acatctcacc cacattcacc
     1381 ggctcatagg tggcagtacg cagtttatgc aaattgccga gcatgcttac tacttgccca
     1441 ccaatgaaag caatattgaa cctgttcttc agttttgtgc tgaacaacac attgattgcg
     1501 cactggtgcc agatgcccag cgcctaagcc atattgggct ggcggtaatg gacatggatt
     1561 ccacactgat ttcaattgaa tgtattgatg aaattgccga catgatgggc attaaaccgc
     1621 agattgccga aattactgaa gccgccatgc gtggcgagtt agactttgcc gccagcttgc
     1681 gcaaacgcgt gagtttactc aaaggcttac ctgaaagtgc gctgcaacgc gtgattgatg
     1741 aacgcctgca actcaacccc ggcgcgcgcg aatggattgc cgcttgcaaa gccaacaaca
     1801 ttaaaaccat ggtcgtttca ggtggtttta cactgtttgc caaccacacc caatcgttat
     1861 tggggttgga ttacgccgtt gcaaatacac tcgaaatcat tgatggcaag ctcacaggcc
     1921 atattttagg cgacatcgtc gatgccagcc gtaaagcgca tgaactcgaa aaactgcgcg
     1981 aagcattagg cctcaacgtc agtcaaacca ttgccattgg cgatggtgcc aatgacctaa
     2041 aaatgatgtc aaccgctggc gttggcgtgg cttaccacgc aaaaccagtg gtgcaagcac
     2101 aagcgactta cgcactcaat tttgtgggtt tagatggcat tgctaaccta tttaccgctt
     2161 aatttaataa caaaaaaatc aacctcaaaa acatcttttt aaagaaaact tttcatcgag
     2221 aaaaaataat gcctctgcga gcctttattt ataaggcttg cagagcatcg aaaaaggttg
     2281 ctgtggaatt taaacgtttt tgcttgtttt tgaatgtcaa aatttaacgc gattacacga
     2341 ccatgttttt attttttaac tggtattcaa ctcggctaat acacaaacga cttaataaaa
     2401 ttcagttcac caattttacg tagcggcacc acaaaatctt cgcgtttttc gttgggcgtg
     2461 ttttcattaa atactagggt aatgcgtgtg gtgatgatgg gtttttgcag ttggctttgg
     2521 tcaaaattgt agccggcttc gttgaggttt ccccaataat tgatgtaaat attgtaagcg
     2581 ccgcgaatgg gagttgacgt gctaaacatt tcagggcctg ggccgtccac gctgtctaca
     2641 tccaacccgc cacccgtttt tagtatgggg cttgaccatg tgacatgctg gccatctggc
     2701 gttagcacat gcaaatcgac ttcagcttct ggggcatccc atgcgcaaat gatgcgcaat
     2761 tttggcagtt gctttgttgg gttagactca taaaactgca cgcgcctaac gctagtgccg
     2821 ttaggtgatt tcagctccac actatttgag ccgctgccaa acgcgtaatg gcgttcataa
     2881 cgcccctccg cgcctacgtt cagcggcaag gggtttccat tcaccacaag tttaggtgtg
     2941 cgattggtcc ccgccgccgc aatacggcct ttgattagcg tgcggttgcg ctgtgcgcca
     3001 cggtcaatcg gcggtgtagg gtaggccacg cgcgcctcac ctgcgcggtc agtcatacca
     3061 gaataattcc acccgccacg cggtgactct aacgtgtttg cagtatcggc agcagcaatt
     3121 tgcacatgta acgcacttaa tactaaaagt atcatcaagc agttagcgtg aagttgcatg
     3181 cgcacgttgg gtcgactcac tgtcatttaa aaaatttgca aatagattga atgggtagat
     3241 taccataatc cctcactgaa atgtttggcc ttaatttgat taaattttct aatccatgta
     3301 atctttaagg gtatttgtat tcatcaagac ttaagtattc accttgctag ccgtaattga
     3361 actatccaat gctaaatacc tctttcaaat tcatattttg atgattaatt aatgaaaaag
     3421 cgcctgcaat ttctagtgca acgcattcaa gccctgcgtg cagaacacga taaggtcgcg
     3481 caagctgctt tgcaagatac tttacagcgc caacctttat tattaggctt ggcgatattt
     3541 tctatgctgc tcggcgcaaa agtgaactgg caaagtatcg gtgcgcttgc acctacggca
     3601 ctacagggta tacaccatgc aagctcacta aatctcatta tggcggtttg gttgatatca
     3661 gcaggcctct ttttaaattg gggacgaagc aaatactgca atactaccgc cctgctagcg
     3721 ctggctacga gctttttaat tttcggcgta ctgcgcgctg ccattgatca atggggaagc
     3781 ggctcagtca acatgttctc actttgttgc gtttttactg gaaccctcat attaataagg
     3841 ccagcgcatg caattgcact ctacgccggc agctacctgc ttttttatat tgcaatggct
     3901 ttgacgcaaa aaaaccctgt catccttgcc atcaatcaag cacaagggtt tgccgcagcc
     3961 gcgcttggcc tgctccttgc gtttttgctg tggcgtaaac atacagtcac caccttgctg
     4021 caacaacaat tacaagccag caacgaagaa ctgcaacggc aaaaacaaca gctagaaatt
     4081 ctttcacaac tagatgcact tacaggctta tataaccgcc gtgagtttga ccgactcgcc
     4141 aatattgaac tagtcagagc caagcgcgaa ggcagcgata ctgccattat catggccgat
     4201 ttagactttt ttaaacgcat taatgattgt tttggccatc caacgggcga tgaagtcatt
     4261 caaaaaaccg cagaagcact cagccaaaat atccgcgctt ccgacatcgt ggcgcggatt
     4321 ggcggtgaag aatttattat attactgccc aaaacctcac ttgacgaagc tttggtcgtg
     4381 gctgaaaaac tacgccttag cctacaagaa aatgctattc aaacgcaaat tagccaaacc
     4441 attcaaatga ccgccagctt tggcgtcacc gccatggcca cgcatcaaca aggttcgctc
     4501 aatgcgcttt atgcggcggc cgacttagcc ctctatgcgg ccaaaaataa aggcagaaat
     4561 cgagttgaat cacaaaacat cagctaaatc ttatccatct tgcttaccaa agtaacaaat
     4621 ttccattcac attttccgtc tgaatcattg atatgactga taggaaaccc ctcttaagcc
     4681 accaaaaata tccatcgaca aacgtgtcta tttgatgctt ttatatacga gaaaaaaact
     4741 ctgccctgcg agccttgtaa ataaaggctc gcagggcagt caaaaaggtt tgtctacaaa
     4801 aactcacaaa aaatcttaaa ttaatctgtc taactgcatc taaaaaacgc atctgcaaat
     4861 cactttaact acaaaataaa accgaccaac gttaatttat tttggttgaa ttaaacctta
     4921 ttctggctag tatcaaccac ttttaaaatc aatcgtgatg aacaacacat tagctcaaat
     4981 attcatttga ccagcgcaat ctgatgtgcc taatatgcgt gtaacgcatt cataatcaat
     5041 aacaaaatgt ctaatttgag tactgctgta ggtgctgagc gccgtcgtta tgagcgtttt
     5101 ccgtttcgca gaatggtcac cctgaatttt tatgaaggcg cgcccattga gttacctgca
     5161 attgacatca gcgctggtgg aattggcgta cgctcgcaca tcaacattgc taccgataaa
     5221 atgtgtatgg caaatatttc tatcgttatt ttgcagggca atttatcttt tgaggctagc
     5281 gctcaggtga aacacagtat tttcagccgt agtgaaaatg gctttattct tggtctcaat
     5341 tacgtaaaaa ttgaacccag cctgcaaacg gccattgtgt gtttgatgcg gggcttgagt
     5401 gaacagataa acacaaccca tgatgctatt gccagattag gccgacatca cacgctttaa
     5461 atgcgcttaa cgggcaagca tttcttgcgc atgctgacga gtggtttccg tgagttctag
     5521 gccgcccagc atacgcgcga tttcttcgac gcgtgatgtt tgattcaatg taacgattgt
     5581 acttaacgtt tgtccattga cgagcgactt actcacgcgc aagtgttgcg acccaagtgc
     5641 cgccacttgt ggcagatggg taatcaccaa cacttggcgt gcctggccaa gttgcttaag
     5701 cagttggcca accacttcag ccacgccgcc gccaatgccc acatccactt catcaaatat
     5761 catactgttc atagaatggc ggtccacact gctctgctgc gcggttacta cccgaatggc
     5821 caaactgata cgcgataatt cgccgcctga agccgctttg tttaacggtt ttggcgcaac
     5881 acccgcatgg cctgccacca aaaattcaac ctgctctaac cctgttgcag acttttcttg
     5941 tggggtcagt aacacttcaa acttgccgcc tgcaagcgat aaccgctgca tttcagcagt
     6001 cactttttca ctcaactgtt gagcagcttt ttgccgacca gtgctgagtt tttcagcatg
     6061 cacatgatat gtggcaaggg cagcagcttc tttttttaac agttcaccat catcgcctac
     6121 actcgccaat tctgccatgc gcgcctgcca ctgggcaagt aaggtgggca acatgtctac
     6181 cgtgctacga tattttcggg cggcagcatg taaggcttga atgcgcgcct caatttctgc
     6241 tagctgtgca ggttccagtt ctgttttttg cacatagcga tttaaaaaac ggtcaacctc
     6301 ttctagctga ataatggcgg aatctaaact ggccagcggc tcatctaatt gcgcatcaaa
     6361 gtcacgcagc atttgcagtt tgtgctgagt ggttgccaat tgccgtagtg ctgaaatttc
     6421 gccctcactt aacaacgcgc gacaggcctc acagccactt aataagcttg cgccatgcga
     6481 taagcgactg tgttcttgct gcaagttttc ccattcgtcc ttccagccat cggttgccaa
     6541 acccaattgg ctcaactcac gcgttttatc gcgtaactcc gcgagttcat ccgcatattt
     6601 actggcgttc tgttccattt caaggcgcgt acggtgcaat tgatgccaaa ctttgaacga
     6661 ctccgccacc gcttgcgcta aatcgcttgc cccggcaaag tcatcgagta catttctttg
     6721 cgtggctaat ttcaatagcg agtgatgcgc attttggctg taaatatcca ctaaatattc
     6781 accaatttct ttgagttggg caaccgttgc gccgatgcca ttgatgaacg cgcgacttcg
     6841 gccatctgaa taaatcacac ggcgtaatac cagctcattt tcatccgttt gcatttcatt
     6901 ggcattgagc cacgcttgcg catccaagtt gtcatgcagg ctaaaatgcg cggtcatttc
     6961 agctttgtcg cacccagccc gcactacatc accctcgttt cgtgcgccca gtgctaaaga
     7021 tagtgcatca atcaatatcg atttacccgc gccagtctcg cccgtaagca cggtgtaacc
     7081 gcgctcaaac tctaggttta attggtctac gatgacaaaa tcacggatag ataacgcttg
     7141 taacatatac ttgaaattag attgctaaga ttgaaaataa gacattattt gggctaggtt
     7201 gcatgctgtt agccccaatg cagttttttg cgcagcattt cataatgaca atgcccgttt
     7261 ggatgcaaca agcgcgtcat tttctctgcc cgcgcaatgt aaacatcgtc atattgatat
     7321 aatcgcgcaa tcaattgccc gtcaaaatga atacgcgatt cgtattgatc aaccagtttc
     7381 acgtgcactt gactggtgct tttaatcaca atcggccgat tactcaatgt gtgtgggcaa
     7441 attggcacaa gggtaatggc ctcgagcgcg gaatgcaaaa tcggcccccc tgctgaaagc
     7501 gcataagcag tggttcccgt tggggtcgcc acaatcaagc catctgaacg ctgacgatgc
     7561 acaaactgct catcaatcat cacctctaaa cccagcaatt gcgccacttc acctttgcta
     7621 atcaccacat cattgagtgc cacgccctcg cccagcagtt tgcctttacg caaaatttta
     7681 gtatgcaaca ttaaacgttg ttcaatgctg taatcgcctg ccagcacttt agcaacatct
     7741 tccagcatat tttgcgcact aatatcagtt aaaaaaccaa tgcgtccgcg gtttattccc
     7801 aaaagaggta cttggtgttc aagcaacgct cgcgcaacgc ctagcatcgt gccatcgcca
     7861 cctagcacga tggctaaatc aatatcgcca ttaaactctg ttaatgcccg aatggggtag
     7921 tcagcaatct cgcaacaacg cgcagtctcc gtttccaaaa aaacatgaag gtgacgcgcg
     7981 cgcaaaaact cagccagcgc aacaatatgc tcacgcattt cacggtcttg gtatttacca
     8041 attaaagcta ttttattaaa tggcgtgttc acatgtttga atctaaatac agactgcctt
     8101 tagattcact ccctaaggtt aagttaaaaa cataagccga attaaaacat agtcactcga
     8161 catagtcact aagcacttaa aaggcaacgc cactttattt acaaaaaaat gtgcatttaa
     8221 tgcatgaata ctgcatggtt tgcatgtaac agataaatgc atagggcttt cattcagaca
     8281 caataattgt taataataat taaatacata taaatcataa tgttatataa ataaaagatg
     8341 catttttagt cgctaaaaat gtgaggcata gttattgcaa tcattgccca acacaattga
     8401 cttgaaaaat aaaaatgcca tccacaacgc aaaaaaaaat acttacgctt tatttcgcca
     8461 tgacttattt tctggcgttg tggctggttt ctgactccat ctcgtttagc agtgcatttg
     8521 agcaaagttg gctgacgctt ggatttatgt tgttagcttt tttgttgtat ggcctttatt
     8581 atttattacc ctcaatttta atcacagcgt tgatgggcca attcactcga cctaacaact
     8641 cgcgccctgt ctacatcaca gccatcatca ctggtgccat caccacttta ttgctctatg
     8701 ccaatgctaa gatttttgcg ctgtatggca tgttttttaa tggctttata ctcaacttag
     8761 tcatgtcacc gggcggcatt gaatctctgg gtggaagtac cgcgtctgac gtcggctacg
     8821 cgcttattgc tctcggcttt gtgctggtgc agattttgat tttatggctg gcaaaacgct
     8881 tttaccaata taaaacacaa actgttttgt cgtttaaatg gctacccatc acagcctttg
     8941 tcgccaccat tttagtacat atgggttttg cacttgatag ctatacaacc acccagttga
     9001 atgtggtggc agaatcagta ccgttttatc agacggtttc tgcgcgtagt tttttctcct
     9061 cactagggtt taaaacacag cgtgacacca aactaaaagt aaaaggtaag ctcaactatc
     9121 cacttaatcc gattcatttt caacaaccag ccaagcccta caatattatt tggctgacct
     9181 ctgaatcatt gcgtgctgat atgctggatg aaaaaatcat gccaaactct tgggcatttg
     9241 ctaaaaatgc cgcgcgcttt acccgcaatt acagcactgg caacggtacg cgcatgggcg
     9301 tatatggcat gtttacgggc acaccaggca attactggtt ttctttttta gagtcccgtc
     9361 gaggcgcatc cattattgat gtattgcaac aacaacacta tcaaatgagt ttctatacta
     9421 gtgccaagtt ttcttatcct gaatttgata agtctatttt cgcaagagtg cctacagacc
     9481 aattgcatga aattgaaaag ccgaaatctg gctgggaaag tgatcgcgac aacgttagcc
     9541 acttacttgc atttattgac caacgaaaca ccaccaaacc gttctttacc tttatgtttt
     9601 ttgaatctcc acatgcgcgt tattactttc cgccagagag cgtcattgca aagccgtatc
     9661 gtgatgattt aaattatgca acattgagta aagaacagct acgcaacgac attgtgccca
     9721 tcaaaaatcg ttatattaac tcggttcatc atttagatgc gcaatttggc cgcatttatg
     9781 attatcttag acaacaccag ctactggaaa acaccatcgt catcatgaat ggtgaccatg
     9841 gtgaagagtt tatggaaaaa agtttttggg ggcataactc aacctttgtt gatgagcagg
     9901 tcaagacgcc tctagtgatt tacaagcccg gcatgcagcc attggtgagc aatcaaatga
     9961 ccagccatat ggatgtgatt ccaaccatta tgccgttatt gggcgtcacc aatccaagca
    10021 gcgactacgc aactggctat aacttactgg caggtgaaaa acgtacccat acttatatat
    10081 cagattggga caaagttgcc tacgtggatg atgaagtgaa gattacgcag cctgtcaacg
    10141 gcaaaagttt tgcgttggtg aaagccagca aaggcgatga cagcgcatta acgccagaag
    10201 aacgcaaagc cgtgttagcc aaaaaacagc ccgccatgct gcaactggtc aatgacttaa
    10261 gcaaattctt taagaaaaaa gaaaccagcc cccaaccgca gtagtattgc ttaatagcgc
    10321 taactcaccc ataatctgcg ggtggatttt agctatggat aaacgcgcac aaatattact
    10381 caaaaccttg gttgagcatt acattagtga tggccaaccc attggctcac gcacgctatt
    10441 acagcatagc ggtttagatg tcagtgctgc taccattcgc aatgtgatgt ccgacctaga
    10501 acaattgggt ttcattacca gcccgcatac atctgccggg cgcattccca cacaaaaagg
    10561 ctatcggttg tttgtagatt cgttacttac cgtgcaaccg cttgaaaaca aaacagtaca
    10621 gcaactcaaa agcggcttaa cctcacccaa cccaaccgaa ctcattgcca atgctgctga
    10681 tatgctgtcg cagttgagcc attttgccgg cttagtggta acacccaaac gaaagcgcat
    10741 tgcttttaaa catttggaat ttttagcatt gtctgaaaag cgcattttgg tcattattgt
    10801 cacgtcagac aacaatgtac aaaaccgtat catcttgagc gaaaaaccct ttaccgccag
    10861 tgaactcaca caagctggca actattttaa tgcgcattat tctggctata catttgaaga
    10921 agtgcagcaa aaactgcaca ttgaactcaa acaaatgcaa accgatatga acaaactgat
    10981 gagtgcagcg cttgaagcca gcaaccaaac agatgcagat gatggtgttg tgattgccgg
    11041 ggaacgaaac ttattgcatg tggatgaact ctcaactaac gttgccagtt tgcgcaagct
    11101 ttttgatatc tttgagcgca gaacttcact gatgcaattg ctcgataaca gccagcgtgc
    11161 agaaggcatt caaatattta tcgggggcga gagcggttat ttgccacttg atgaatgctc
    11221 aatggtcacc gcgccgtatg aagctgacgg acaaattgtg ggcacattag gcgtcattgg
    11281 ccctactaga atggcgtatg agcgtgtgat accgattgtg gacgtgacag ctaaattatt
    11341 atcgaacgca ctttcaacta actaaaaaaa gaactgttaa attcaatgcc cttttacaac
    11401 aaagaacccc aatacgaaca cggcgaccaa ctaaaagtag gcatcttact cgccaactta
    11461 ggcacgccag acgcaccaac cgcccaagca ctacgtccct acttaaggca atttttgagt
    11521 gatcggcgca ttgttgaaat tccgcgcgtg atttggtggt ttattttgaa ctgcattatt
    11581 ttagtggtgc gccccaaaaa atctgccgaa aaatacgcac aggtgtggac taaagaaggc
    11641 tcgcccctat tggtacacgc gcaaaaacaa acgcaattac tgcgtggcta cctcagccaa
    11701 aaaatcaact caccgcatgt ggttgaacta ggcatgagct acggcaaccc cagcatggca
    11761 agcgctattg aaaaactaaa agctgcccat tgcgaccgta ttttagtgtt tccactttac
    11821 ccgcaatatg ccgccagcag cacggccagc gcgctggacg cggtgtggcg cacattatta
    11881 aaaatgcgca atgtgccagc gattcgctcc atacgcagct atcacgacca ccctgcttat
    11941 attgcggctt tggcaaaaag cgtacaagaa cattgggtta tccaccacaa acctgacttt
    12001 agcagtggtg aaaagttggt catgagtttt catggcgtac ccaaagtgca tctcaccagc
    12061 ggcgacccct accactgcca gtgccacaaa accgcacgct tattggctga agcactaaat
    12121 ctcaacaaaa ctgattatat cgtggctttt cagtcacgct ttggcaaaca agagtggcta
    12181 aaaccctacc ttgccagcac cttagaagat ttaggcaaag ccaagaccaa acgccttgat
    12241 gtcatttgcc ccggtttttc aagcgactgt ttagaaacac tggaagaaat cgccatggaa
    12301 ggcaaacata tttttcaaag caacggcggt ggtgaataca attacattcc cgctcttaat
    12361 gaaaatgatg cctggataca ggccatgacc accattgcgc tagaaaatct gcaaggctgg
    12421 gtgcgtgctg attgggatag tgaagctgca aaactgcaaa atacgcgcac taaaaataat
    12481 gccaaattac tgcaacccta acggcatttt ttagctataa aaatgatgtt ttttgatacg
    12541 ttattcgccc gagcaaaaaa cactgccctg cgagccttgt aaataaaggc tcacagagca
    12601 cctcaaaagg gttggtgtgt ttgcaaagca ataaatgatg tttttttaaa taccattaga
    12661 agataaaaat acccacgctg gaaagctgag aaatacacat aacatgcatt tcacgccttt
    12721 tcgatataga acataaaccg gtgcactacc aatacaaatt ccatgcaaaa taaaaccaag
    12781 ctttttttaa ttccgctttt ttgtatgagc ttaaatgcgt gcacggcgct taaaaacctc
    12841 agccaacaac attctccaca aaacacgatt aacaccacca aagtagctga tcgttggcaa
    12901 gcaacacttc cgcatggtgg caattcggca aatttagcgc aattctggca gcaatatcaa
    12961 gacccgctac tgctgaaact cattgaagcg gcacaaaaag aatcgtcgtc aattgcgact
    13021 gccaattcac gcattgccca agcccgcgcg acgcgcacgc aggccaatgc agcgctcacg
    13081 cccacggtgg atggctcagt ttcggcatca cgcagtgtgc agcaaccggc aaccagtatt
    13141 ggcggctcac agcaaggcaa tattggtggc ggcagtggcg cattcaacaa tgcacaaatc
    13201 agcgcgcaag ccagttggga gctagatatt tttggcgtca accgtggtgc ttttgaagcc
    13261 agcaaagcgc aagaaaatgc aacgcttgca agctggcacg aagcgcgcgt gtccgttgcg
    13321 gcagaaactg cgaatgctta ttttaatcag cgcttttgca gcacgcaggc taacgtgctc
    13381 gaagcggatg tgatatccag aactgaaacc ttgcggctca ccaacattgc agtaaaggcg
    13441 gggttttctg cgcctgcaaa tagccatttg gctgaagcaa gcttggctga tgccaggcag
    13501 caacgcacag cacaaatggc gcagtgtgat ttagacgtaa aagcgctggt ggcacttacc
    13561 gccatagatg aagcgacttt acgtgaaaaa ctaaaccagc aagattttgt gcgcctgcat
    13621 gaatccacca acaatctttt tacactcaat gaaattccag cacaagtcat cgcccagcgg
    13681 ccagatattt acgcagcaga agcagattta gtcagtgctg ctgctgatat tcaaaccacc
    13741 tacgccagca gcttgccaaa agtatcactc aatggctcaa taggctggat gtggttgtca
    13801 ggcagcggct ttagcaccaa tggtaaaacc tggtcattgg ggccaatttc aatcactttt
    13861 ccgatttatc acccaggtgt caaagaggcc acgcttgcca gcagtgaggc aaaatatgaa
    13921 gaaaaagcga gcatttatcg cagtaaggtt agaactgccg tgaaagaagt ggaagatgcg
    13981 ctggtaaact tacatagtgc caattcgagg caggctgaca ttgcgcaagc cattcagagc
    14041 tatcaggcct cgtttgatgc aatgcaaaca aaggtaaatg caggatttgc caatttaatt
    14101 gacttggaag atcagcgccg tgttttactc aatgcgcgca ccaacgccat taacaaccaa
    14161 aaacaacaag ccgtggcttg ggtgagcttg tatcgcgcgg ctggcggcgg ttgggacaaa
    14221 acggaaaaat tcaaaacaga acagttagag actggaagta ccccatgatt tcaaaaacac
    14281 ttattccact gttgattgct gccagcctgt tgcttggcgg atgccaaagt gaacaagaca
    14341 gcagccctga aaccacgacc agcaaagaag ccgttgctaa agctagcctc actgtcagca
    14401 cgattaagcc gcaacaaaca gcactgaata aaaccttaaa tgccaatggc aatattgctg
    14461 cctggcaaga atcgagcata agcacagaaa caaacggttt gcttttgcgc gaagtgttag
    14521 tgggcattgg cgaccaagtc aaacgcgggc aagtgcttgc gcgctttgct gccgccagca
    14581 ttgaagcaga agtggcgcaa acacaagcca atgtggcgga ggcacaagcc accctcattg
    14641 aggcagctgg caacgccaat cgtgcgcgta gcattcaaga ttcaggcgcg ttaagccgcc
    14701 aacaaattga gcaatacatc actgcagaag ccagcgcaaa agcacggctg gacgcagcac
    14761 aagcgaattt acgcgcacag cttattaaaa tcaagcaagc aaccgttacc gcgcctgatg
    14821 atggcattat ttcgcaaaac cctgccaccg ttggtacggt atatgccgct gggcaagagt
    14881 tatttcgcct gattcgaaaa ggtcgcattg aatggcgcgc agagcttacc tcctctgatg
    14941 tgtcgtttat taaagcgggc atgcaagcac aaatcacctt gccagacggc gctacagcac
    15001 aaggtaaggt acgcaccatc gcgccaagtg tagataacaa aacccgcaac gccattgtgt
    15061 atgttgatat tcctgctggc agcgcaaaaa tcggcatgtt tgcgcgcggc caatttttac
    15121 tgggcaatac cgatagcctc actctgccca acgatgccat tgtgatgcgt gatggctttg
    15181 cctatgtgat gcaggtacac aataataaaa tcacccaaac aaaagtacaa ctcggccagc
    15241 gcgcagggga tcaggttgaa ttactcaact ttaacgctgc aaatgcagaa attgttgcca
    15301 gtggcggcgc atttttgacg gatggtgatc gcgttagagt ggtgaataaa agtgtaggca
    15361 accaatgaat atttccagct ggtcaatcaa aaacccggtg cctgcactga tattatttat
    15421 actgctcacg ctagcgggca gtatgagttt taacagcatg aaagtgcaaa attttcctga
    15481 tttagactta cccactgtca ccgtcaatgc gtcccttcct ggtgcatcgc cagaccagct
    15541 tgaaactcaa gtcgcccgca aaattgaaaa tgccgttgcc aaactgcagg gtttacgcca
    15601 catttacacc accattcagg atggcaccgt acgcattacg gctgaattca ggcttgaaaa
    15661 accagtacaa gaagctgtag acgatgtgcg ttcagccgtt tctgaagtgc gtgccgattt
    15721 acccagtgat ttacgcgaac cgattgtcag caagctcaat attgcaagtt ctcccatttt
    15781 ggccttcacg atacattcta aacgtatgga tgatgaagcg ctttcatggt ttttggatga
    15841 tgcgctcacc aaaaaactgc taggcgtaaa aggcgttggt aatgttgcgc gcgttggcgg
    15901 tgtcaatcgc caaatatggg ttgaactcga caccgctaaa ttgcaatcac ttggtaccac
    15961 tgccgccgaa atttctcgaa atttagggcg cattcagctt gaagtagcgg gtggcaccac
    16021 taaattaagc gggagcgaac aacctattcg cacgcttgcc aacgtgaaat cagccgatga
    16081 attagcgcaa atagaaattg cacttaatga cggcagaaaa attcgtttaa atcaagttgc
    16141 caatgtcttg gataccattg ccgagcctaa atcggccgcc tttttaaacg gccaaagtgt
    16201 gattggtttt gaggtaagcc gcagcaaggg cgcaagcgag cttgaagtcg gcgctggcgt
    16261 ttacgacgcg ctcaaggcca ttcaactgca ataccccgat attgaaatca ccgaagcctt
    16321 taattttgta cagcccgtgc aagatgaatt tgatgcgtcc atgaccatgc tctacgaagg
    16381 tgcgttacta gcagtcttag tggtgtggct attcttgcgt gattttcgtg ccacctttgt
    16441 ctcggccgtg gccttgccct tgtctgtcat tccggcgttt atcggcatga aatatatggg
    16501 cttttcagtc aatattgtga ccttactcgc actgtctttg gtggtagggg tgctggtgga
    16561 cgatgccatt gttgaggttg aaaatattgt ccgccactcg cgcatgggaa aacccccttt
    16621 tgaagccgcg ttaatcgcca ccgaagaaat cggtttggcc gtggttgcta ccacattcac
    16681 gctcattgcc gtatttttac ctaccgcgtt tatggatggc attccaggaa aattctttaa
    16741 gcagtttggc tggacagcag catttgccat ttttgcatcg cttttggtcg cccggctact
    16801 cacgccgatg atggcagctt acctcatgcg acccagcaaa gtagaaactg aaacggatgg
    16861 tgttgtcatg cgcacttata tgcggcttgc gcgttggtgc gtacagcatc gtttaatgac
    16921 cacggttgcg gccattttat tttttatagg ctcactttca ctcatccctt tattacccaa
    16981 aggatttgta cccgcagatg acaactcgca aacacaagtg cgcattgagc tagcgccggg
    17041 tgcaacctta gcgcaaacca ttgccataag tgaacaagcg cgccgcatat taaaagacgt
    17101 agcgcatgtg aatagtgttt acacggctgt tggtggcggt gcggcaggca gcgacccatt
    17161 tgcaggtggc ggcacggctg aaacccgcaa agccacactg acgattcaac tagacccgcg
    17221 cggtaaacgg ccgaaaaaac aagtcattga agctgaaatc cgtgagaaat tacaagccct
    17281 agcaggggtg cgcctaggag tgggcttaga aggttctggc gaaaaatatg tactcgccct
    17341 caccagtgaa gatgccaccg ccttgcaaaa tgccgccagc gcagtagaaa aagacctacg
    17401 caaaattcaa gggctaggca acattgcctc gagtgcagct ttggttcggc ctgagattat
    17461 tgttaaacct gattttgcaa aaacagccga ctttggcgtc accagcgcgg ccattggcga
    17521 aaccctgcgc attgcaacga ttggcgatta cgaacagttt ttacctaaac tcaatttgga
    17581 acaacgccaa atacccattt tggtgaaact caataacgat gcgaggcaag acttgaatat
    17641 tcttggcaaa ctcgccgtgc caagctcaaa tggcagcgtc gcgctagaac aagtcgccag
    17701 cctgcaaatg tcaagcgggc cagcggtgat tagccgttat gaccgctcac gcaacataca
    17761 atttaacatt gagctttcag gccttgccat gggcgatgcg gttgaagccg taaaaaacct
    17821 gccgagtatt cagcatttac cagcaggcgt catgcaaacg gaactaggcg atgctgaaat
    17881 gatgggcgag cttttttcaa gctttggcat tgccatgctc acgggtgtcg tttgcattta
    17941 ctttgtactg gtattgttat ttaaagactt tttacagccc atcactattt tagtcgccct
    18001 gcctttgtca cttggcggtg catttttagg gctgctcgtc acaggtaaag cactgtcaat
    18061 gccctcgatg attgggctgg tgatgctcat gggcattgcc accaaaaatt caatct
//
LOCUS       DAOQSI010000022        17941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_2243_length_17941_cov_5.023203, whole genome shotgun sequence.
ACCESSION   DAOQSI010000022 DAOQSI010000000
VERSION     DAOQSI010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 17941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17941
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2243_length_17941_cov_5.023203"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="PKL42_02770"
     CDS             <1..1115
                     /locus_tag="PKL42_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNU66024.1"
                     /translation="LRVLLVQALFANPDILLLDEPTNNLDINTIRWLEDVLNNRDCTM
                     IIISHDRHFLNQVCTHTCDMDYGKITVYPGNYDDYMEASTQARAQQAKDNAKAKQQIA
                     DLQDFVRRFSANASKAKQATSRAKQIDKIKIEEFKPSSRQYPFIRFEYDERDKLHRNA
                     VELKKLSHGFDKLLFNDVNMMFEAGEKVAIIGENGIGKTTFLRCLARDLQPNHGEVKW
                     AEKANIGYFAQDHEYEFEDGEDLFEWMTKFRQPGDDDQVVRSMLGRLLFGGDDTKKSV
                     KVLSGGEKGRMLYGKMMLARTNVMLMDEPTNHMDMESIEALNTALDKYKGTLFFVSHD
                     REFVSSIATRIIEIKADGIVDFTGNYEDYLASQGVE"
     gene            1127..1525
                     /locus_tag="PKL42_02775"
     CDS             1127..1525
                     /locus_tag="PKL42_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66025.1"
                     /translation="MLKALWIIFLIIVWFFTLVMHSAVFQSYSFRGIAYLLACIWLIY
                     LAGLWFEQSRMTDAAWAMLLGYAIGMIRLLDLLPNQQQYANWLKYVPKFIVGGAALIF
                     ILIFVGLLPLIAKNIIASHKAKNRVLGRED"
     gene            1912..2055
                     /locus_tag="PKL42_02780"
     CDS             1912..2055
                     /locus_tag="PKL42_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3149 domain-containing protein"
                     /protein_id="HNU66026.1"
                     /translation="MQLLTNLLSTDYGLQSLAVIVFILGMVVWFGFYFKRHMDEDARN
                     AEK"
     gene            2413..3822
                     /locus_tag="PKL42_02785"
     CDS             2413..3822
                     /locus_tag="PKL42_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNU66027.1"
                     /translation="MFWHSTPSLAEESIWYRTNDPLNTDAQLPPAIPAGYIEDTACKI
                     RTYDQALTLADVAEAALCNNPQTRSAYASARVQAAQLGIAKSAYFPTVNDNVSANLNV
                     ALPEQIARNNPYSNLSNNIVASYLLYDFGNRDANLENARQLLFAASATQNSVVQTVLL
                     SAIQAYYQTQANAAALEAAITSEHASEESFKAAEARYKAGVSTPADKLQAQTLWAQNT
                     LSRISAEGALKTAYGTLANVMGLEANQSVKLAASSSIQPGQHITEDINALIEQARVRR
                     PDLLASEAQLKAAQASIDANRAAAKPTVSVALSNNLQDGSQVAFGSNSSLGVTVSIPI
                     FAGYGPTYKIRSAEASADLRATQRDQLKLQISLDVWRAYQNLRTANESIVAANALVSS
                     ATESSLVALGRYKAGVGNIIDAINAQSALASARQQQVQATLNWNIARATLAQSIGGLE
                     YAMIQSLPAGALPTSAVEK"
     gene            3825..5069
                     /locus_tag="PKL42_02790"
     CDS             3825..5069
                     /locus_tag="PKL42_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNU66028.1"
                     /translation="MQKTIKKIIIFAVLIGLVAGGYYYFQKRTTVPLEQRYRTQAITQ
                     GDVAQSVSANGTLNPVTLISVGAQVSGRVSKLYVDFNDHVEKGQILLELDDALFAAQI
                     AQSQGAVQNVQASVDLAQANEARMQTLFAQEYVSKQELDQATQALKSARAQLATARGQ
                     LQRDKTNQSYSVIRSPVSGVVVDRVVDVGQTVAASFQTPTLIKIAQDLSKMQIDTSFA
                     EADIGNIKVGQAVKFNVDAFPNRSFNGVVKQLRLNPTVTSNVVTYNVVVSVDNPEQIL
                     LPGMTAYVNIMVNKHDNVLLVPNAALRFKPKLDDTQSNGSASAGKTSTDKNIDANQSN
                     NGQMRGRKKASQDDISRGKIYIIADGKLTPIEVKTGISDGRTTEISSPTLKVNTQVIV
                     GENQTDETAASTPTSNMRFRMF"
     gene            5069..5788
                     /locus_tag="PKL42_02795"
     CDS             5069..5788
                     /locus_tag="PKL42_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNU66029.1"
                     /translation="MRLVQVHHLHKDYTTIAGTIHVLKDVNFVMDAGDFVAIMGPSGS
                     GKSTFMNILGCLDQPTSGEYLLDEHSVATLNANALAQVRNQTIGFVFQGFNLLSRTTL
                     LDNVALPLVYSKISKNERLARANSLLEKVGLGQHLYSFPNQISGGQQQRVAIARALVN
                     RPRLILADEPTGNLDSATSKEIMTLFSELNAEGISIILVTHESDIAAHASRQVRFLDG
                     RIVHDERSKSEIQLTEAAETS"
     gene            5788..7008
                     /locus_tag="PKL42_02800"
     CDS             5788..7008
                     /locus_tag="PKL42_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNU66030.1"
                     /translation="MFSAMLGEAWRAMGANRMRTFLTMLGMVIGVGSVVLMMAVGQGA
                     QESVKQSINAMGSNLFIVLSGSSTSGGARSGSGNTSTLTVADANAIAELDGVSNVAPI
                     SMGNAQVVYGNTNWSTSIIGTTPSYLDVRVWELADGYVFSESDMRSATRVALVGKTVV
                     DNIFGDTDPIGKTIRIKQNPFVVLGVLKRKGQSLDGRDQDDTIIMPLTTAQRKILGNQ
                     FSGSVRQVMVQANTPELMPSVEQSINALLKQRHNIREGVDNDFNVRNLTAVANSAAET
                     TRTMSLLLGAIASVSLLVGGIGIMNIMLVSVTERTREVGIRMAIGARERDILLQFLLE
                     AIVISVIGCLIGLVLGISSAVLVNTITQAAVVISGYSVMIAFAVAASVGIFFGYYPAR
                     KAARMNPIEALRFQ"
     gene            complement(7184..8998)
                     /gene="typA"
                     /locus_tag="PKL42_02805"
     CDS             complement(7184..8998)
                     /gene="typA"
                     /locus_tag="PKL42_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNU66031.1"
                     /translation="MSRNLRNVAIIAHVDHGKTTMVDKLLQQAGTFSSHQAVTERVMD
                     SGDIEKERGITILAKNTALTYEGTHINIVDTPGHADFGGEVERVLGMVDGVVLLVDAV
                     EGPMPQTRFVTKKALALGLKPIVVINKVDRPGARTDWVINQTFDLFANLGATDEQLDF
                     PIVYASAINGYAMLDMNKQTDNMRALFETIISYVSPPVGDSSAPLQMQISALDYSTYT
                     GRLGIGRITNGKVKPGQAVAVMNEDKQIATGKINQVLGFKGLERVPVEEAEAGDIVII
                     SGLDDIGIGFTVCDRENPKGLPHLGVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDR
                     LTKELLVNVALRVDETEDNDVFRVSGRGELHLTILLENMRREGFELAVGKPKVVYREI
                     NGEKCEPYEILTVDLEDEHQGAAMEELGRRRGEMQNMESDGNGRTRLEYRIPARGLIG
                     FQGEFLTMTRGTGLMGHVFDEYAPVKADMPGRRNGVLISAEHGEAVAYALWKLQDRGK
                     MFSVPGDKLYEGMIIGVHSRDNDLVVNPIKGKQLTNVRSSGTDEAVRLITPIALSLES
                     AVEFIDDDELVEITPKTIRIRKRFLTENERKRSGTNKS"
     gene            9398..11116
                     /locus_tag="PKL42_02810"
     CDS             9398..11116
                     /locus_tag="PKL42_02810"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNU66032.1"
                     /translation="MSFESLNLNPAILRAIEESGYTTPTAVQAQAIPVVLEGHDLMAS
                     AQTGTGKTAAFMLPALNLLATPHESKSRGPRILVLTPTRELAAQVNEAARKYGKHMRA
                     RTVSIVGGMPYPLQNKLLSQPLDILVATPGRLLDHMERGRIDLSRLQMLILDEADRML
                     DMGFLPDVERICEQLTPERQTLLFSATLDGDIARIAKQILKNPKSIQVAAQREKHANI
                     EQRLHYVDDMTHKNKLLEHLLIAPEVNQAIIFTSTKRHADVLAEDLYAAGHKTAALHG
                     DMTQGARNRTLTKLRHGDVKVLVATDVAARGIDVHGITHVINYDLPKFAEDYVHRIGR
                     TGRAGNTGIAISFASNVDRHLLRKIEQFTGNRMEAAVIEGFEPKRPMKSDAPSGKRGQ
                     DRNARNERSHAPGGRGGFKPREERSFGRNENRFEGRSETRGNRESAPREANGNSAGFA
                     PRPEFNRSERPASDRPRSFGDRNANGGRDRANTERSNAPAPNRSFGDAAAFGKKPFPR
                     DGQNSAPRNDNRVLGARFEGRADTARRDSRTDSRPAGRNHSDSRSFGNANAGGSSAPR
                     RPRSFA"
     gene            11143..12558
                     /locus_tag="PKL42_02815"
     CDS             11143..12558
                     /locus_tag="PKL42_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNU66033.1"
                     /translation="MTENVISETEVTQLLNFDNLGLAPELLKALSESGYTTPTPIQVQ
                     AIPVALAGGDVMAGAQTGTGKTAAFALPLLQKLLPLANTSTSPAKHPVRALILTPTRE
                     LAIQVEASVKAYAKHTALRSLVVFGGVDIKTQTPHLKTGVEVLVATPGRLLDHIEQKT
                     LQLNQVQMLVLDEADRMLDMGFMPDLKRILALLPKQRQNLMFSATFSNEIKKLSDDFL
                     TKPTLIEVARSNATNDNVTQQVYQVAQRDKEALLVQLLKEASTSQVIVFTKTKITASR
                     LSRSLAKSGIAADAIHGDKTQKERIEALDAFKAGKIMTLVATDVAARGLDISELPMVI
                     NYEIPSAPEDYVHRIGRTGRAGASGIAISLVSEEEEKYLTEIEKLIKKSIIKITAHIE
                     KPSPKPSSDLQKDSSRHSKTTRTSHEGATQRMPAKKKATDPWFDKPYEASNQIQAANA
                     RPASTRKTAVAALLGGLVSNK"
     gene            complement(12685..13116)
                     /locus_tag="PKL42_02820"
     CDS             complement(12685..13116)
                     /locus_tag="PKL42_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HNU66034.1"
                     /translation="MLNKIEETGSDKRTFFELLGGEENGIANIRQLVETFYDVMESDP
                     KVAPIRAMHQPDLTSAREKLFMFLTGWTGGPQLYIERYGHPMLRARHLPFPIDASARD
                     QWMYCMIKAMHQLEFDENLMTKLASQLYGVADFMRNQAEKN"
     gene            complement(13214..14413)
                     /gene="purT"
                     /locus_tag="PKL42_02825"
     CDS             complement(13214..14413)
                     /gene="purT"
                     /locus_tag="PKL42_02825"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HNU66035.1"
                     /translation="MQIGTPLSPNATRVMLLGSGELGKEVIIALQRLGVEVIAVDRYE
                     NAPGQQVAHRAYTIDMTDNQALRDLVAKEKPHLIVPEIEALNTSTLADIEAAGIATVI
                     PTVKAVQLTMNREGIRRLAAEELQLPTSPYAFARSEVELQAAIDAGIGYPCFIKPTMS
                     SSGKGQSRITQADEVKTAWEYAGKGGRVNQGVVIVEGQIDFDYEITLLTVRAKNAQGD
                     IQTYFCEPIGHVQKNGDYVESWQPQAMTVGALEESKRIAKAVTDSLGGLGLFGVELFI
                     KGDKVWFSEVSPRPHDTGMVTMCSQRFSEFELHARAILGLPVDVSLREAGASAVIYGQ
                     HETKSLAFEGVDAALAVPNSDIRLFGKPESFVKRRMGVALATGSDVDEARSRAILSAS
                     FVKPKIA"
     gene            complement(14529..15995)
                     /locus_tag="PKL42_02830"
     CDS             complement(14529..15995)
                     /locus_tag="PKL42_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNU66036.1"
                     /translation="MQLAHIAQGSTTVVLKQQSKILIVEDEVLFARAVMRRLQKAGYE
                     CEHVESLLAAAAALKQFSPDLVLLDMRLPDGNGLDLLADLVAKNAAVIVMTAHGEVSD
                     AVNAIKLGAVDYLKKPIDLEELLLSVQKAEAAAVQSNSLDYSRQRNAHAAEGVELLGD
                     SVAIQSVKSQIQRIAQLVSSDTVPPTVLIGGETGTGKDVAARLLHLSCANKDKPFVHI
                     DCASLPAELIESELFGHEKGAFTGAQGARPGLIEAAEDGTLFLDEIGELPLTLQAKLL
                     NVLERRIVRRLGSTKERAVPARFIAATNRDLHEMSAAGRFRQDLYYRLNVMSIHMPPL
                     RDREDDAFLLSKHFAAQTAKRYGLASPVFLADAVERIRQYQWPGNVRELRHQVSRAML
                     LSHQGRIAAVDLALPMLPNGAQSVDMVTNNTLADYQAYTQISSATQNQVTLYEAEKNI
                     LLKALAEAEYNVSKAARKLGITRMTMRYRMDKHQIPHK"
     gene            complement(16019..>17941)
                     /locus_tag="PKL42_02835"
     CDS             complement(16019..>17941)
                     /locus_tag="PKL42_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNU66037.1"
                     /translation="GLNTLGGALSVQTKNGRTHQGGGIETSFGSWSRKTVAAEFGGVS
                     ADNSIDYFLAGNYFDEEGWRDHSPTEVKQVFGKLGWQNEASRLELSYTGADNDMIGNG
                     LIQKELMEDFGRETINTKPDQTKNTLSFLNLNGSHWLNDDVQLSANTYYRKSKRKTLN
                     GDINDDYDLLATGTVAGNDADCPLGTGDEDELCSGALNRSNTDQKGYGFNAQLAFNQP
                     LLEKQNQLIVGLGYDYSRVKFNQSSEFGIVNASRGVDGIDVENDEAEVDLLGKTRSWG
                     VFATDTLSLTNQLHLTLSGRYNHIKVKNTDKINPKGSLEDVDGDPLTPDEDVSLSGNH
                     SFSRFNPAIGLSFTPTRDLSVYGSYNEGMRAPTSMELGCANPNVPCKLPNAMAGDPPL
                     DEVVSKTFELGARGNLTSDVKWSAAVYRTENHDDIHFISTNATNGMGYFDNVGKTRRM
                     GLDAGLSGAFGSFSWNAGYSYLKATYESDLELTNSLNSTSDGNAIQVNKGDRLANLPE
                     HSLKLRLQLQATPNWSIGTNINTFSDVYIRGNENNKHRANDGDADHVQDSGKIGGYTV
                     VNLDTRYKFGDSGWQVFAKAINIFDKEYATGGMLGENWIEDGVFAGDDEPSKMLMPGV
                     PRAGWIGVRYDFGGKK"
BASE COUNT         4967 a         4338 c         3842 g         4794 t
ORIGIN      
        1 aattgcgtgt tttgctggtg caagccctat ttgccaaccc agatattctg ctgctcgatg
       61 agccaaccaa caacttggat atcaacacca ttcgctggct ggaagacgtg ttgaataacc
      121 gtgattgcac catgattatc atctcgcatg accgccactt tttaaaccaa gtctgcacgc
      181 acacgtgcga catggattac ggcaaaatta cggtataccc cggtaattat gatgattata
      241 tggaagccag cacacaggcg cgcgcgcaac aagccaaaga caacgccaag gccaaacaac
      301 agattgctga cttacaagac tttgtgcgcc gcttttctgc caacgcctct aaagccaagc
      361 aggccaccag ccgcgccaag caaattgata aaattaaaat tgaagaattc aagccttcta
      421 gccgccaata tccctttatt cgttttgaat atgacgagcg cgacaaactg catcgtaatg
      481 cagtggagct aaaaaaactc agccacggct ttgacaaact gttattcaac gacgtgaata
      541 tgatgtttga agccggcgaa aaagtcgcaa taatcgggga aaacggcatt ggtaaaacca
      601 cctttttacg ctgtttagca cgcgacctac agcctaatca cggcgaagta aaatgggcag
      661 aaaaagccaa tatcggctac tttgcgcaag accacgaata cgaatttgaa gatggcgaag
      721 atttatttga atggatgacc aaattcagac agcccggtga cgacgaccaa gtggtacgca
      781 gcatgttagg caggttatta tttggtggtg atgacaccaa aaaatctgtc aaagtacttt
      841 ctggcggaga aaaaggtcgc atgctttacg gcaaaatgat gttggctaga accaatgtga
      901 tgctgatgga tgaacccacc aaccacatgg acatggagtc gattgaagca ctcaacaccg
      961 cgctagacaa atacaaaggc acgttatttt ttgtcagcca cgaccgcgaa tttgtaagtt
     1021 ccatcgccac acgcatcatt gaaattaaag ccgatggtat tgttgatttt accggcaatt
     1081 acgaagatta cttggcgagc cagggcgttg aataaggcat agaggtatgc tcaaagcgct
     1141 atggattatc tttttaatca tcgtctggtt tttcacttta gtgatgcata gcgcggtatt
     1201 tcagtcatat agctttcgcg gcattgccta tttacttgca tgtatctggc tgatttactt
     1261 agctgggttg tggtttgagc aaagccgcat gacagatgct gcatgggcca tgctactggg
     1321 ctatgccatc ggcatgatac gtctgctaga tttactccct aaccagcaac aatacgctaa
     1381 ttggttaaaa tacgtaccaa aatttattgt cggtggcgct gctttaattt ttatcctgat
     1441 atttgtcggc ctattaccgc tcatcgccaa aaatataata gcctcgcaca aagcaaaaaa
     1501 tcgcgtattg gggcgagagg attaattttt cggacttgaa attttctata gctaaatcag
     1561 tttaaataac gcgacttccc aagcccattc aacacctcac aacacttcaa aaaacctagt
     1621 ttttcactaa aaatcaagcg caaccttttt cgatgccctg agagccttat aaataaaggc
     1681 tctcagggca ataaaatttt ctcgcgtgat ttaatcgctt ttgggtgcaa tttaaaagcc
     1741 tactaaataa cctaaaatac gaaagtattt tatttgttaa taatgcctct agattttatt
     1801 taactccctt ttgattttta tcaatactac attcctatga caggagcata ttgatgacaa
     1861 cagagcattt tataagtttt tctgttaatt tagcctagag gagttagcat tatgcaatta
     1921 cttacaaatt tactttctac tgattatggt ctgcagagtc tcgctgtgat tgtatttatt
     1981 ttaggcatgg tggtgtggtt tggattttat ttcaaacgcc acatggatga ggatgcgaga
     2041 aacgctgaaa aataaatcca agatttggta ggcctacaaa ttacgaaatt tcaagggtct
     2101 ttttttaaag gcagatttaa cgtctgcctt gttgtattga gcctttcact tatttcacac
     2161 ttcgtgtgtc actccagcat tgttgatatt tttttatatt aacacttcac gattttcatt
     2221 tttgaatcta agccgttaga ctgaagatgc atgttagaat gaaaccagat gccgaactaa
     2281 cgttagcgca tcttgtcttt tctagcgagt attaaaacct cacatcgcct aatatcagag
     2341 caaatgccaa ctttcattcg cccacaaagt tcatccatct ttcatatatt ggtactgtgt
     2401 tttgccaatt taatgttctg gcactcgact ccaagtctgg ctgaagagtc tatttggtat
     2461 cgtactaatg acccactcaa cacagatgca caattaccgc ccgctatccc tgctggttat
     2521 atagaagaca ccgcttgtaa aattcgcact tatgaccaag cactcacatt agccgatgta
     2581 gctgaggccg ccttgtgcaa caacccgcaa acccgcagtg cttatgcaag cgcacgtgta
     2641 caggcggcac aacttggtat tgcaaaatct gcgtattttc caacggtcaa tgacaacgtc
     2701 tctgccaatt taaatgttgc cttacctgaa caaattgccc gcaataaccc gtacagcaat
     2761 ctaagcaata atattgtggc gtcttacctg ctgtatgatt ttggcaatcg cgatgccaat
     2821 cttgaaaatg cgcgccaatt attattcgcg gccagtgcca cacaaaatag tgtggttcaa
     2881 actgtattgc tcagtgcaat tcaagcttat tatcaaacgc aggccaatgc agcagccctt
     2941 gaagccgcca ttacatcaga acacgccagc gaagaaagct ttaaagcggc tgaagcacgt
     3001 tataaagccg gcgtttcaac cccagccgat aaattgcagg cgcaaacact ttgggcacaa
     3061 aatacgctgt caagaatcag tgctgaaggt gctttaaaaa ccgcctacgg cacactcgcc
     3121 aacgtgatgg ggttggaagc caatcaatca gttaaactgg cagcaagcag ttccatacaa
     3181 cccggacaac atattactga agacattaac gcgctgattg aacaagcgcg cgttcgccgc
     3241 cctgatttat tggcgagtga agcacagtta aaagcggctc aagctagtat tgatgccaac
     3301 cgtgcagccg caaaaccaac ggtttcagtg gcgctatcca acaacctaca agatggcagc
     3361 caagtggctt ttggtagcaa ctcctcactt ggcgtcaccg tttcaatccc aatatttgca
     3421 ggttatgggc ctacttacaa aattcggtct gccgaagcca gcgcagactt acgcgccact
     3481 cagcgtgatc agttaaaact gcaaatctca ttagacgtgt ggcgcgctta tcaaaatttg
     3541 cgcactgcca atgaatcaat cgttgcggca aatgcgctgg tgagcagcgc aacggaatcg
     3601 tcacttgtgg cacttgggcg ttataaagca ggtgttggca atatcattga cgctatcaat
     3661 gcacaaagcg ctttggcaag tgcgcgtcag cagcaagtgc aggccaccct caattggaac
     3721 atcgcgcgcg ccacgcttgc acaatccatt ggcggactcg aatatgccat gattcaatct
     3781 ttacctgcgg gcgcactgcc cacttctgct gtggaaaaat aagcatgcaa aaaaccatta
     3841 aaaaaatcat tatatttgcg gtgttaattg gtttagtagc tggtggctat tattactttc
     3901 aaaagcgcac cacagttccg ctagagcaac gctaccgaac acaagcaatc acgcaaggtg
     3961 atgtggcaca aagtgtttcg gccaatggca cgctaaaccc tgtcacgctg attagcgtag
     4021 gcgcccaggt ttcaggccgc gtcagcaagc tttatgtaga ctttaacgac cacgttgaaa
     4081 aaggccaaat cttacttgag ttagatgacg cactatttgc cgcgcaaatt gcccaatcgc
     4141 aaggtgcggt acaaaatgtt caggcctccg ttgatttagc acaggcgaat gaagcccgca
     4201 tgcaaaccct ctttgcgcag gaatatgttt ctaaacaaga acttgaccaa gcgactcaag
     4261 cgttgaaatc tgcccgcgca caattagcca cggcgcgagg tcagttgcag cgtgacaaaa
     4321 ctaaccaaag ctactctgtg atacgttcgc cggtttctgg tgtggtggta gaccgtgtgg
     4381 tggatgtggg gcagacggtt gccgccagtt tccaaacgcc aacgctgatt aaaattgcgc
     4441 aggatttatc taaaatgcaa attgacacca gctttgccga agcggacatt ggtaatatta
     4501 aagtggggca agccgttaaa tttaatgtgg atgcttttcc aaaccgcagt tttaatggcg
     4561 tagtcaaaca actgcgcctc aacccaacgg taacgtcgaa cgtggtgact tacaatgttg
     4621 ttgtttcggt agataacccc gagcaaatat tactgcctgg catgacagct tatgtgaata
     4681 tcatggtcaa caaacatgat aatgtgctgc ttgtgcccaa tgccgcgctt cggtttaaac
     4741 caaaattgga tgatacgcaa tcaaacggta gcgcaagcgc tggtaaaaca agtacagata
     4801 aaaatattga cgcgaatcaa tcgaataatg gccaaatgcg tggcagaaaa aaggccagtc
     4861 aggacgacat ttcacgcggt aagatttaca tcattgctga tggaaaactc acgccgattg
     4921 aagtgaaaac tggcatttcg gatggccgga cgactgaaat cagcagtcca actctaaaag
     4981 tgaataccca agtcattgtg ggcgaaaacc aaactgacga aactgctgcg agtacaccga
     5041 ccagcaatat gcgctttagg atgttctaat gcgtttggtg caagtgcatc atttgcacaa
     5101 agattacacc accatcgccg gcaccatcca tgtgctgaaa gatgttaatt ttgtgatgga
     5161 tgcaggtgac tttgtggcga ttatggggcc atccggctct ggaaaatcca cttttatgaa
     5221 cattctaggc tgcttggatc aacctaccag cggtgaatat ctgttagatg aacattcagt
     5281 agcaacgctt aatgctaacg cactcgccca agtgcgtaac caaaccatcg gctttgtatt
     5341 tcaaggtttt aacttactct cgcgcacgac cctgttagat aacgtggcgt tgccattagt
     5401 ctattcaaaa atcagcaaaa atgaacgctt ggcacgggca aattcgctat tagaaaaagt
     5461 gggcttaggc cagcatctat attcgtttcc aaaccaaatt tcaggcgggc agcagcaacg
     5521 cgtggccatt gcgcgtgcgc tggtgaatcg gccacggcta attttggctg acgaaccaac
     5581 aggtaaccta gacagcgcga ccagtaaaga aattatgacg cttttttcag aattaaatgc
     5641 tgagggcatt tcaatcattt tagtcacgca tgaatctgat attgccgcgc atgccagccg
     5701 tcaggtacgg tttttagatg ggcgcattgt gcatgatgaa cgcagcaaat cagagattca
     5761 actgacagaa gctgcggaga caagctaatg ttttccgcga tgttaggtga agcttggcgc
     5821 gccatgggtg ccaatcgcat gcgtacgttt ctcactatgt tgggcatggt gattggcgtg
     5881 ggttcagtgg tgctgatgat ggccgttggg caaggcgcgc aagaatctgt gaaacaatcc
     5941 attaacgcca tgggcagcaa tctttttatt gtgctttcag gctcatccac ctctggcggt
     6001 gcacggtcag gcagtggcaa caccagcacg ctgacagtgg ctgacgcgaa tgccattgcc
     6061 gagttagacg gggtaagcaa tgttgcgccc attagcatgg gcaatgcgca agtggtgtac
     6121 ggcaatacca attggagtac aagcattatt ggtacaacac caagttactt agatgtacgc
     6181 gtgtgggaac ttgctgacgg ctatgtattt tcagaatctg acatgcgttc tgccacccga
     6241 gtcgcgctcg taggtaaaac ggtggtagat aatatctttg gcgacactga ccccatcggc
     6301 aaaaccatcc gcatcaaaca aaacccattt gtggtactcg gcgtacttaa acgtaaagga
     6361 caaagtctgg atggccgcga tcaggatgac accatcatca tgccactcac caccgcacag
     6421 cgcaaaattc ttggcaacca attttctggc agcgtgcggc aagtcatggt gcaggcaaat
     6481 acccctgaat tgatgccaag tgttgagcaa agcattaacg ccttactcaa acagcgccac
     6541 aatattcgcg agggggtgga caacgacttt aatgtacgca acctcaccgc ggtagccaat
     6601 tcggctgcag aaactacgcg cactatgtca ctgttactcg gcgcgattgc ttcggtttca
     6661 ctcctggttg gcggcatcgg cattatgaat atcatgctgg tatctgtaac ggaacgtacg
     6721 cgagaagttg gcattcgaat ggctattggc gccagagagc gcgacatttt gttacagttt
     6781 ttacttgaag caattgtcat ttcagtcatc ggctgtttaa ttggtctagt tcttggcatc
     6841 agcagtgctg tgctcgtcaa taccattacg caagcggctg tggttatttc tggttattcg
     6901 gtaatgattg cgtttgccgt agccgccagt gtcggcatat tttttggcta ctatcccgcc
     6961 cgaaaagcag cccgcatgaa ccccattgaa gcgctacgtt ttcagtagaa aatctgcact
     7021 ttttaagcac catttgaatg cttaaatttt aatcagctaa aactcccatt cggagcaaaa
     7081 tttggcaata aaaaacccag cgttttgcgc tgggcttatg tttatcctac tactagcaga
     7141 ctattaaata ctaagatttt tttaaagtag gattttttta atactagctt ttattagtac
     7201 ctgaacgttt acgctcgttt tctgttaaaa aacgtttacg aatacgaatc gtttttggcg
     7261 tgatttctac caattcatca tcatcaataa actcaacggc tgattctaag cttaaagcaa
     7321 ttggcgtaat taaacgtacc gcttcatcag tgcctgatga gcgcacgttg gtaagttgtt
     7381 tgcctttaat tgggttcact accaagtcgt tatcacgact atggacgccg ataatcatac
     7441 cttcgtataa tttatcacca ggtactgaaa acatttttcc gcggtcttgc aatttccaca
     7501 gcgcgtaagc aaccgcttcg ccatgctcag ctgaaatcaa cacaccatta cggcggcctg
     7561 gcatatctgc ttttactggt gcgtactcat caaacacgtg acccattaag cctgtaccgc
     7621 gcgtcatggt taagaattca ccttgaaaac caatcaaacc acgggctgga atgcgatact
     7681 ccaaacgtgt acggccgttg ccgtcagatt ccatattctg catttcacca cggcggcgac
     7741 ctagctcttc catggccgca ccttggtgtt catcttctaa atccactgtt aaaatttcat
     7801 acggctcgca cttttcaccg ttaatttcac ggtaaaccac ttttggctta ccaaccgcca
     7861 attcaaaacc ttcacggcgc atattttcta acaaaattgt taaatgcaat tcgccacggc
     7921 cagaaacgcg gaatacgtca ttatcttcag tttcatcaac gcgcaatgcc acgttgacta
     7981 ataactcttt ggttaaacgg tcacgaattt gacgactagt gacaaattta ccttcagtac
     8041 ccgccaaagg tgaagtgttc accataaagt ccatagtcaa ggttggctca tccacgccta
     8101 aatgcggcaa gccttttgga ttttcacggt cgcacacggt aaagccgata ccaatatcat
     8161 ctaaaccaga aataatgaca atgtcacctg cttcagcttc ttctactggt acgcgctcaa
     8221 gtcctttaaa accaagaact tggttgattt tacctgttgc gatttgcttg tcttcattca
     8281 ttacagcaac ggcttggcca ggtttcactt taccgttggt aatacggcca atgcctaaac
     8341 gacctgtgta agttgagtag tccagcgctg aaatctgcat ttgtagtggc gcgctactgt
     8401 cgcccacggg cggtgataca tagctgataa ttgtttcaaa caaagcacgc atgttatcgg
     8461 tttgcttgtt catgtctaac atggcataac cattaatggc ggaggcatac acaatcggaa
     8521 agtctaactg ttcatccgtt gcgcctaaat tagcaaataa atcaaacgtt tggttaatca
     8581 cccaatccgt acgcgcacca ggacggtcta ctttattgat gaccacaatt ggttttaacc
     8641 caagtgccaa agcttttttc gttacaaaac gcgtttgcgg cattgggcct tcaaccgcat
     8701 caaccaacag caccacacca tccaccatgc ccagcacacg ctcaacctcg ccaccaaagt
     8761 ccgcgtggcc cggtgtatcc acaatattga tgtgtgtgcc ttcataagtc agggcagtgt
     8821 ttttagccaa aatggtaata ccacgctctt tttcaatatc gccactgtcc atcacacgct
     8881 cagttaccgc ttggtgggat gaaaaagtac ctgcttgttg taaaagttta tccaccatgg
     8941 tggttttacc atggtcaacg tgggcgatga ttgcgacatt acgtaaattg cgagacatat
     9001 atttgtagcc agttcattga aaggcggcga ttttaacaca ttaaccacaa taaacctttg
     9061 acaattttaa atttgtgcct ataatgcgca caacttaaaa cttaaatgta atttatagtt
     9121 ttagattcat tgccctgacc ctctgtcact cgtgaacttt tgaagtgact attctttaga
     9181 tttgattcca ttaaatctat cttttactgg ttagcggctg tgcccgtgcg ctggtaaata
     9241 aagcttgaag aatttatttt aaagtcttta aaaattgctt tttgcgttat gtttttttgc
     9301 gtggtgtcat tgatttaaca caactgcaaa ctagaatgca tttactcgcg ccctttatta
     9361 ttgtttttta taaaaacagc ttttgaaagg gactactttg tcttttgaat cattaaattt
     9421 aaatcctgcc atcctacgcg ctattgaaga gtctggttac accaccccta ctgccgtgca
     9481 agcgcaggca attcctgtgg tattagaagg ccacgacctg atggcctcag cccaaactgg
     9541 tacaggtaaa accgctgcat ttatgttgcc agcattgaac ttgctcgcaa ccccacacga
     9601 atcaaaaagc cgtggcccac gcattttagt gctaacgcca acccgtgaac tcgctgcgca
     9661 agtcaatgaa gctgcccgta aatacggcaa acatatgcgc gcccgtacgg taagcattgt
     9721 gggtggcatg ccatacccac tgcaaaacaa attattatca caaccgttag atattttagt
     9781 cgccacccct ggccgtttgt tagaccacat ggagcgcggc cgcattgatt tatctcgctt
     9841 gcaaatgctt attttagatg aagcagaccg catgctggat atgggctttt tacctgatgt
     9901 tgagcgtatc tgtgagcaat tgacgcctga gcgccaaacg ttgttattct cagccacatt
     9961 agatggtgac attgcacgca ttgctaaaca aatcttgaaa aatccaaaat caattcaagt
    10021 ggcggcacaa cgtgaaaaac atgccaacat tgagcaacgt ttgcactatg tggatgacat
    10081 gacacacaaa aacaaattgc ttgagcattt gttaattgcg cctgaagtga atcaagcgat
    10141 tattttcacc agcaccaaac gccacgctga cgtattggct gaagacttat atgctgctgg
    10201 ccacaaaacc gctgccctac atggcgacat gacgcaaggc gcacgtaacc gtactttaac
    10261 taaactgcgc catggtgacg ttaaagtgtt agtggcaacc gatgttgctg cgcgcggtat
    10321 tgatgtgcat ggcattacac acgtaatcaa ttacgatttg ccaaagtttg ctgaagatta
    10381 cgtgcatcgt attggccgta ctggccgtgc tggtaacaca gggattgcca tttcatttgc
    10441 ctctaacgta gaccgccatt tgctacgcaa aatcgagcaa tttaccggca accgtatgga
    10501 agctgccgtg attgaaggct ttgagccaaa acgcccaatg aaatctgacg caccaagtgg
    10561 caaacgcggt caagaccgca atgcgcgcaa tgagcgttca catgcaccag gtggtcgcgg
    10621 tggttttaaa ccacgtgaag aacgtagctt tggccgcaat gaaaaccgtt ttgaaggtcg
    10681 tagtgaaacg cgcggtaacc gcgaatctgc tccgcgtgaa gccaatggca attcagcagg
    10741 gtttgcacct cgcccagaat ttaaccgctc tgaaagacca gcaagtgacc gtccacgtag
    10801 ttttggtgat cgcaatgcca atggtggtcg tgaccgtgcc aatactgaac gctcaaacgc
    10861 acctgcgcca aaccgcagtt ttggtgatgc cgctgctttt ggcaaaaaac cgtttccgcg
    10921 tgatggccaa aatagcgcgc cacgcaacga caatcgcgtg ctaggtgcac gttttgaagg
    10981 ccgtgcagat actgctcgcc gtgatagcag aacagatagc cgccctgctg gtcgtaatca
    11041 cagcgattct cgcagctttg gcaatgcaaa tgcaggtggc agcagcgcac cgcgtcgtcc
    11101 acgtagcttt gcataatttt catattggat aaatttcaac acatgactga aaatgtaatt
    11161 tctgaaacag aagtaacgca gcttttaaac tttgataact tgggcctcgc gcctgagttg
    11221 ctcaaagcac tcagtgaatc tggctatacc acgcctacgc ccatccaagt gcaagctatt
    11281 ccagttgcat tggctggggg cgatgtgatg gcaggcgcgc aaacaggcac aggtaaaact
    11341 gcggcgtttg cattgccttt gttgcagaaa ttactgccgt tagccaacac cagcacttca
    11401 cctgctaaac accctgtgcg ggcgctcatt ttaacgccca cgcgcgagtt ggctattcag
    11461 gtagaagcca gcgtaaaagc gtatgccaaa catactgctt tacgctcgtt ggtagtgttt
    11521 ggaggggtgg acattaaaac ccaaacgccg cacttaaaaa ccggcgtaga agttttggtc
    11581 gcaacgcctg gtcgcctgct ggatcatatc gaacaaaaaa ctttgcagtt aaatcaagta
    11641 cagatgctgg tactcgatga agctgaccgt atgttagaca tgggctttat gcccgactta
    11701 aaacgcattt tggcgctgtt acccaaacag cgccaaaatt taatgttttc tgccacattt
    11761 tcaaatgaaa tcaaaaagtt atcggatgac tttttaacga aaccaacctt gattgaagtg
    11821 gcgcgcagta acgcaaccaa tgataatgtg acacaacaag tgtaccaagt tgcgcaacgt
    11881 gataaagagg cactacttgt tcagctactt aaagaggcaa gtacgagcca agtgattgtg
    11941 tttaccaaaa caaaaatcac ggcaagccgc ttgagccgta gcctagccaa aagcggcatt
    12001 gcggctgatg ccattcatgg cgataaaacg cagaaagagc gcattgaagc actcgatgct
    12061 tttaaagcag gcaaaatcat gaccttagtt gcaaccgacg tagcagcaag gggcttggat
    12121 attagcgaat tacctatggt aattaactat gaaattccga gtgcgcctga agactacgtt
    12181 caccgtattg gccgcacagg ccgcgcagga gcaagtggca ttgccatttc attggtgagt
    12241 gaagaagaag aaaaatactt aactgaaatt gaaaaactga ttaaaaaaag tattattaaa
    12301 atcacggctc acattgaaaa gccaagtcca aaaccgtcat ctgaccttca aaaagatagc
    12361 agtcgtcaca gcaaaacaac gcgcaccagc catgaaggtg ccacacagcg catgccggca
    12421 aaaaagaaag caactgaccc ttggtttgat aaaccttacg aagcaagcaa tcaaattcaa
    12481 gcagccaatg cccggcctgc ctctactaga aaaactgccg ttgctgcgtt gctgggcggc
    12541 ttagtttcta acaagtaatt tagcacaacc tttttcgatg ctctggaagc cttataaata
    12601 aaggctcgca gggcataaat ttttcctcgg ggataaatta aggctttttg tagtgtttta
    12661 tgtgtgtcat atacacctaa aacactagtt tttttctgcc tgattacgca taaaatcagc
    12721 cacgccatac aactggctgg ccagctttgt catcaagttt tcgtcaaact ccaactggtg
    12781 catggcttta atcatgcaat acatccactg gtcacgcgca gaggcatcaa taggaaacgg
    12841 caaatgccgc gcccgcaaca tggggtgtcc atagcgttcg atatagagtt gcggcccgcc
    12901 tgtccaaccg gttaaaaaca taaacagttt ttcacgcgct gaggttaagt ctggctggtg
    12961 catggcgcgt atcggtgcca ctttggggtc gctttccatc acatcgtaaa aggtttctac
    13021 taactggcga atattggcga tgccgttttc ttcaccacca agcagttcaa aaaatgtgcg
    13081 tttatcgcta cctgtttctt caattttatt gagcattatt ttcagccacc atttgtagga
    13141 gcaattttta aatcgcgaag atcaggatga ttataccaac attactttcg cgatttaaaa
    13201 atcgctccta cagctaggct attttaggtt taacaaaact tgccgacaat atcgcgcggc
    13261 ttcttgcttc atcaacatca ctacccgtag ccaaagccac gcccatgcgg cgtttcacaa
    13321 aagactcggg tttaccaaat aaacgaatgt cactgttggg taccgctaag gctgcatcta
    13381 caccttcaaa cgccaaactt ttcgtttcat gttggccgta aatcaccgca ctcgcgcctg
    13441 cttcacgcag gcttacatcc acgggcaacc ctaaaatggc acgcgcatgc aattcaaact
    13501 cactaaagcg ctggctacac attgtcacca tgccggtgtc atgcgggcgc gggctaactt
    13561 cactaaacca gactttatcg cctttaataa acagttctac accaaataag ccaagtccac
    13621 ccaagctatc cgtcaccgcc ttggcgatgc gttttgattc ctccagtgcg ccaactgtca
    13681 tggcttgcgg ttgccagctt tctacataat cgccattttt ctgcacatgt ccgatgggtt
    13741 cacagaagta agtttgaata tcgccttgtg catttttagc acgcactgtc agcaaggtga
    13801 tttcataatc aaaatcaatt tgaccttcca caatcaccac accttggttc acgcgccctc
    13861 ccttacctgc atattcccaa gccgttttca cttcatcagc ctgcgtaatg cgcgattggc
    13921 ctttgcctga tgacgacatg gtcggtttga taaaacaagg gtaaccaatg cctgcgtcaa
    13981 tggccgcctg tagttcaacc tcacttctgg caaacgcata gggtgaagtg ggtaattgca
    14041 attcctccgc tgccagccga cgaatgcctt cacggttcat tgttaattgc actgctttta
    14101 cagttggaat tactgtggca atgcccgctg cttcaatatc ggccaacgtg ctggtgttga
    14161 gcgcttcaat ttccggcaca attaagtgcg gcttttcttt tgctaccaaa tcacgcaaag
    14221 cctgattatc cgtcatatca atcgtgtaag cacggtgtgc tacttgctgg ccaggagcat
    14281 tttcgtaacg gtctaccgcg atgacttcaa cccctaaacg ctgcaacgcg ataatcactt
    14341 ctttgccgag ttcaccactg ccgaggagca tcacacgggt agcgttggga cttaatgggg
    14401 taccgatttg catgatagat ttcctttttt caatgggcta aatcatacca cgcagtgatt
    14461 atgtcgtcat ttgacaaaca tcatcaccat gcaaaaagat aaaaaattat gaaaggattg
    14521 gaactagatt atttatgggg aatttgatgc ttatccatgc gatatcgcat cgtcatacgt
    14581 gtaataccaa gctttcgtgc ggcttttgac acattatatt cggcctctgc caaagctttt
    14641 aacagaatat ttttctcagc ttcatacaat gtcacttgat tttgtgtggc tgaactaatt
    14701 tgtgtatagg cctgataatc agccagcgtg ttgtttgtga ccatgtcgac agattgcgca
    14761 ccgtttggta gcatggggag tgccaagtca acagcagcga tgcgtccttg atggcttaac
    14821 agcatggcgc ggcttacctg atggcgcagt tctcgtacat tgccaggcca ctgatattgc
    14881 cgaatgcgtt caacggcatc tgctaaaaaa acggggcttg caaggccgta gcgtttagca
    14941 gtttgcgctg caaaatgctt ggagagcaaa aaggcatcat cttcacggtc gcgcagaggc
    15001 ggcatgtgaa tactcatcac attgagtctg taatataaat cttgccgaaa gcgcccggcc
    15061 gcagacattt catgcaaatc gcgatttgtt gcggcgataa agcgtgcagg cactgcacgc
    15121 tctttggtag agccaagtct gcgcacaatt ctacgctcta gcacatttaa aagttttgcc
    15181 tgtaaggtga gtggcaactc gccgatttca tccaaaaaga gcgtaccatc ttctgcagcc
    15241 tcaatcaggc caggcctagc accttgcgcg ccagtaaatg cgcctttttc atgcccaaac
    15301 aactcgctct caatcagttc agcaggcaaa gatgcgcaat caatatgcac aaacggtttg
    15361 tctttattgg cacaagacaa atgcagtaag cgcgctgcga catctttacc cgtgccagtt
    15421 tcgccaccaa tcaataccgt tggcggcacg gtgtcgcttg aaaccagctg tgcaatgcgc
    15481 tgaatctgcg atttcacact ttgtatcgca acgctatccc ctaacaactc aacaccctca
    15541 gccgcatgcg cgttacgctg gcgtgagtaa tccaagctat tactttgtac tgcagcagct
    15601 tctgcctttt gaactgagag caacagttct tctaagtcta tcggtttttt aagatagtca
    15661 actgcgccta atttaatggc gttcacggcg tcactcactt caccatgggc cgtcatcaca
    15721 atcactgctg cgtttttagc aactaaatct gccagcaaat ccaacccatt gccatcaggc
    15781 aggcgcatat caagcagtac taaatcaggt gaaaattgct ttaatgcggc tgcggctgcc
    15841 aacaaactct ccacatgctc acactcgtaa ccagcttttt gcaagcggcg catcaccgca
    15901 cgggcaaaca acacctcatc ttcaacaatc agaatctttg actgctgctt caaaacgacc
    15961 gtggtgctgc cctgtgcgat atgcgctaat tgcaaaattt ttccttgatt gactacagtt
    16021 acttcttacc accaaaatca tagcgcacgc caatccagcc tgcacgtggt actcctggca
    16081 tcaacatttt tgatggttca tcatcaccag caaatacacc gtcttcaatc cagttttcac
    16141 ctaacatacc accagtggca tattctttat cgaagatatt gatggcttta gcaaatacct
    16201 gccaaccgct atcgccaaat ttatagcgtg tgtctaagtt aacaacggtg tagccgccaa
    16261 ttttgccgct atcttgcacg tgatcagcat caccatcatt ggcacgatgc ttattgttct
    16321 cgttgccacg gatataaaca tctgagaatg tgttgatatt ggtaccaata gaccaattag
    16381 gcgttgcttg taattgcaaa cgcaatttaa gtgaatgctc tggtaaattg gctaaacgat
    16441 cacctttatt cacctgaatt gcattgccat cgctagtaga gttcagtgag ttcgttaact
    16501 ctaagtctga ttcataggtt gcttttaaat agctatagcc tgcattccag ctaaagctac
    16561 cgaatgcgcc tgataaacct gcatctagcc ccatacggcg ggttttacca acgttatcaa
    16621 aataccccat accattggtt gcattagtcg agataaagtg aatatcatca tggttttcag
    16681 tgcggtatac cgctgctgac catttcacat ccgatgttaa attaccacgt gcacctaact
    16741 caaaagtttt tgaaaccact tcatctagtg gcgggtcacc tgccattgcg ttaggtaact
    16801 tacaaggtac atttggattt gcacagccta gttccattga agttggcgcg cgcataccct
    16861 cattataaga accataaaca gacaagtctc tggttggtgt aaagctcaaa ccaatggctg
    16921 ggttaaagcg gctgaagcta tgattgccag aaagggatac atcttcgtca ggcgtcaatg
    16981 gatcaccatc aacatcctcg agcgagccct tagggttgat cttgtctgtg tttttaactt
    17041 taatatggtt atagcggcca gaaagtgtta aatgcaactg atttgtcaac gacaaagtat
    17101 cagttgcaaa cacaccccaa ctgcgggttt tacccagtaa atccacctct gcctcatcat
    17161 tttcaacatc aataccgtcc acaccacgtg acgcattaac aatgccaaac tcagacgatt
    17221 ggttaaattt cacacgagaa taatcataac ctaaaccgac aatcaactga ttttgctttt
    17281 ctaacaatgg ttggttaaac gccaattgtg cattaaatcc atagcccttt tggtcagtgt
    17341 tactgcgatt gagtgctccg ctacacaact catcttcatc gccagtcccc aacgggcagt
    17401 cagcatcatt accagcaacc gtacctgtgg ccaataaatc ataatcatcg ttaatatcgc
    17461 catttaacgt tttacgtttt gatttacgat aataagtatt cgcactcaat tgcacgtcat
    17521 cattcaacca atggctgcca tttaagttta agaaagaaag tgtatttttt gtttggtcag
    17581 gttttgtatt aatggtttca cggccaaagt cttccatcaa ctctttttga attaagccat
    17641 taccaatcat atcgttatct gcacccgtat aactcagctc taagcgagat gcttcgtttt
    17701 gccagcccaa tttaccaaaa acctgtttca cttcggtagg cgagtgatca cgccagcctt
    17761 cttcatcaaa atagtttcct gcaagaaaat aatcaattga attatccgct gatacaccac
    17821 caaattcagc agcgacagtt ttgcgtgacc atgagccaaa actagtttcg ataccgccac
    17881 cttgatgggt acgaccattt tttgtctgta ctgataacgc gccaccaagc gtgtttaaac
    17941 c
//
LOCUS       DAOQSI010000023        16924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_2478_length_16924_cov_6.140317, whole genome shotgun sequence.
ACCESSION   DAOQSI010000023 DAOQSI010000000
VERSION     DAOQSI010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 16924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16924
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2478_length_16924_cov_6.140317"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /gene="ruvB"
                     /locus_tag="PKL42_02840"
     CDS             complement(<1..1047)
                     /gene="ruvB"
                     /locus_tag="PKL42_02840"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNU66038.1"
                     /translation="MIETDRLIAPETESPREEALERALRPKLLDEYVGQEKARGQLEI
                     FINAARGRSEPLDHVLLFGPPGLGKTTLAHIIAKEMGVNMRQTSGPVLERAGDLAALL
                     TNLEPNDVLFIDEIHRLSPVVEEILYPAMEDYRLDIMIGEGPAARSVRLDLPPFTLVG
                     ATTRAGMLTNPLRDRFGIVSRLEFYTAEELSRIVQRSAGLLEVELVDTGALEIAKRSR
                     GTPRIANRLLRRVRDYAQVKANGKVSAEIADAALKMLDVDKLGFDVMDRKLLQAVLEK
                     FGGGPVGLDNLAAAIGEERDTIEDVLEPYLIQQGYLMRTPRGRVATSLAYQHFGLSVP
                     ANLSTGELWQSVNGN"
     gene            complement(1107..3251)
                     /locus_tag="PKL42_02845"
     CDS             complement(1107..3251)
                     /locus_tag="PKL42_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNU66039.1"
                     /translation="MNNIGIKARVIFLGTVPALLFAIILIGYIISNIFGILNQSLVDR
                     GKIIATQLAPAAEYGVISGNRQMLQKLVQQALDHEQDLKTVMVVDNQGLVLAYSGQAI
                     PETLISKIMQENLPEYKQARGIIFTAPIHRSLVEIDDFSSLEENTQQLKKDFSPEKIG
                     QIYVELSNQGLQSYKLVFVVKIASIALLGLLLSGWLAWRIGRNITQPIQEIARAVNKV
                     REGVLSQTLVENSSGELKTLQKGFNSMSASLKHAYDVMQDKINDATSMLRHQAQHDDL
                     TGLINRREFEVRLERCLKSAKENNAKHVFCFLDLDQFKLVNDTSGHIAGDELLKQVSV
                     LLAIRMRERDTLARLGGDEFGLLLENCTQKEADQICSAIQRIIRDFRYSHDDKIFNIG
                     VSIGMVVIDDHFDSVSEVVHAADMACMAAKKAGRNQTVLFNAGDVEVVEQKNAVEAIA
                     DITDEIDDDQFMLYCQAIVPLTPNLPHLPNMPAIPQQHHYEILLRKVDLDGKIILPTT
                     FIPSAERYHLMPNIDRWVIKNAFIAYRKLLDIDYEHCNYIFSINLSGTSLGDQSLLPF
                     IREQFAKYAIPPASICFEITETAAIVNLKNTILLFNSLREIGCKFALDDFGSGMSSFM
                     YLKNFDVDYLKIDGAFVKEIHQNRIDHAMVRSIHNVAESMNIKTVAEFVESDAILKQL
                     EIIGVHYGQGLHLGLPITVKELIQQYAELHPR"
     gene            complement(3244..4212)
                     /locus_tag="PKL42_02850"
     CDS             complement(3244..4212)
                     /locus_tag="PKL42_02850"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HNU66040.1"
                     /translation="MSQYKNKNSVFSMRFACCFFAVMCLFSEVALAYTGVTIVMSSKT
                     EANLAFVEQFKTELASNENNTLNLKVIELPARKRLVVAENSELVIALGEKALEAASKL
                     SHTTPVLAVHTPSLAYEQILEKSRRDASNMSMIMQNQPYARQINFIQLVLPAAKNIGS
                     LVNGNSQTYAEQLKTQADKKGLNVQLTHLNDPSMLIPELKTLLEANDALLAIPDTAVY
                     KPDTAQSILMTSYQFKKPVLCYSLSYAKAGALAALYSSSRHLAKQAAEIAQQSQNAES
                     ALPLPQAPKYFSVYVNRQVARLLNIEVNKDVAIFQELIATDNLQNE"
     gene            complement(4389..5285)
                     /locus_tag="PKL42_02855"
     CDS             complement(4389..5285)
                     /locus_tag="PKL42_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNU66041.1"
                     /translation="MKRYLIFIVSLAVFLAACGQQQPPVPTVAMAPAPAEMASTKMAS
                     ADSVAVAGEGEQSLTELTEPATAQSEVKRFIALRHHLVVETPTEQMQANFDATVAHCQ
                     QLKCQILSANYNRETAYSPPSASLSARIPPRSVAVFLAGLAKSGEVLQHHRSSEDKTD
                     AVIDAEARIKNLTEFRDNLRAMLSDKSAKFKDLIEVQRELVNTQSELDGIQGVRKVLA
                     QETELVAINIDFTAKQGITEQGFFSPVAQAFKNAGHVMMESLASVITFFVTILPWLIF
                     GIPALILIRKLWAKIRTKKPHV"
     gene            complement(5319..5894)
                     /gene="ruvA"
                     /locus_tag="PKL42_02860"
     CDS             complement(5319..5894)
                     /gene="ruvA"
                     /locus_tag="PKL42_02860"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNU66042.1"
                     /translation="MIARLNGILIEKSPPQIVIDCNGVGYEVEVPMSTFYNLPELGSK
                     VNLLTYFVVREDAQLLYGFGSEQEKSTFKQLLKVNGIGAKSALSILSGVSIDDLIQAV
                     SMQEVGMLTRIPGIGKKTAERLLLELKDKFSVAGLAQTANQAKSATHDILNALVALGY
                     NEREALAAVKLLDKEVSVTDGIKQALKNLSK"
     gene            complement(5891..6847)
                     /locus_tag="PKL42_02865"
     CDS             complement(5891..6847)
                     /locus_tag="PKL42_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HNU66043.1"
                     /translation="MTNNAKLLLEPAIGTYANNITKYFFATRPAFLLAALAACLLGLA
                     SAKLGGVNLNVAFAIATILLALVVHAAVNVLNDYFDALSGADAFNTERVYPFTGGSRF
                     IQNGLFTPQQTAGFGYGLLLVAMLGGLWLVAEVGKGLLLIGGVGLFIGWAYSATPLSL
                     NSRGLGEVCVLLGFLGVVIGADFVQRISFSWQPVFVGFPYALLVTNLLFINQFPDYKA
                     DKLAGKHHWVVLLPLTTAANIYPILVLLACVWLLAMALLQRLPAMTLWSALPLLFALR
                     AAWLLQQHYAEPAKLRPAIQLTLLAMLGHALLLTLLLFWNAR"
     gene            complement(6844..7416)
                     /gene="ruvC"
                     /locus_tag="PKL42_02870"
     CDS             complement(6844..7416)
                     /gene="ruvC"
                     /locus_tag="PKL42_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNU66044.1"
                     /translation="MALVTTRILGIDPGLRLTGFGVIEKVGEKIVYVASGTIKTASGK
                     KNVVEGEADREELPARLKIILDGLFEVIDTYQPTQVAVEKVFVNVNPQSTLLLGQARG
                     AAISAAVIRNLIVAEYTALQVKQSVVGNGHAQKEQVQEMVKRLLNLPATPKPDSADAL
                     ACAICHAHGGQGLGRLATAGFRVKGGRLIG"
     gene            7624..8574
                     /locus_tag="PKL42_02875"
     CDS             7624..8574
                     /locus_tag="PKL42_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66045.1"
                     /translation="MLKTQHMLTKLLTAPFKLFAPLKKFGYRMFFATGVGIAVLFIAF
                     SLLLDKQSVAVLLAAIGAGLGALIISLTLPKLLVSADVERQKQLASEMQKVLKDKAKL
                     VIELERIKSQQMQFQQIQAVLKLTLLEVDLSLTDFKQEDLGEREKTLGGKTSTQYIGV
                     IRKKAKAMLGLDLAHIRVQQIGKELLVDKLRAQYQGVSANTEEWLFRQLQIHENNLVM
                     PNRININLDSNELMHASDAHREDLNQRINNGVELKAFDHAIEKMGEQWLKLMFAPMGL
                     TVRVAQINEEDSVLLLDYIKSTQLHLEQAQHLLEENTALI"
     gene            complement(8728..9453)
                     /locus_tag="PKL42_02880"
     CDS             complement(8728..9453)
                     /locus_tag="PKL42_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNU66046.1"
                     /translation="MAGHSKWANIQHRKGRQDAKRGKIFTKIIKEITVAARLGGGDAD
                     MNPRLRSAVAEAKEENMPSDNIIRAIKKGTGELEGVNYEEIRYEGYGINGAAIIIDCL
                     TDNKQRAVADVRHALTKFGGNLGTDGSVAFMFKHCGNLVFEPGTDEDKVMEVALEAGA
                     EDIVTNDDGSIEVITPPADFVAVKEALEAAGLKAVLAEVTMKALNEVEFTGDDAVKMQ
                     KILDALEDLDDVQDVYTTALIEE"
     gene            complement(9624..10535)
                     /locus_tag="PKL42_02885"
     CDS             complement(9624..10535)
                     /locus_tag="PKL42_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNU66047.1"
                     /translation="MQKNITIKALVALFILTLIWGYNWVVMKLAVQYANPFQFAAMRT
                     FSGAIILFIIMAFTKRAMMVKEFPTMLVLGFLQTIGFTGLIIWAVSIGGAGKTAVLSY
                     TMPFWVMLFAWPMLGEKVQGWQWLAVVSALLGIILIFDPLHIKADAFSMFLAVCSGVF
                     WAISAIISKKLHKRAPHLDLLNITAWPMLLGSIPLVIYALMLDAPPIQWTNTFIAAVL
                     FNVILSGVVAWLLWLYALQRLNAGIASMASMLAPVIGVISAWLQLNEVPGKQELIGMS
                     FIGLALVIISAISIRKHAQVDAAQGQE"
     gene            10622..11053
                     /locus_tag="PKL42_02890"
     CDS             10622..11053
                     /locus_tag="PKL42_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNU66048.1"
                     /translation="MKTLQQLLDSKKHKEVISVAPHRPVFDALVVMAEYRIGALVVLQ
                     GEKLVGIFSERDYAREVVLKGRSSKTTQISDVMTDKVLSAKPSDTVEQAMSIMSEKRI
                     RHLPVVENNQVVGMLSIGDLVKETIDYQQRLIKQLESYIRG"
     gene            complement(11156..12505)
                     /gene="yegQ"
                     /locus_tag="PKL42_02895"
     CDS             complement(11156..12505)
                     /gene="yegQ"
                     /locus_tag="PKL42_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HNU66049.1"
                     /translation="MKTPELLAPAGDLDRMRTAFDYGADAIYAGQPRYSLRARNNDFT
                     MANLEIGIKEAHARGKKFFVASNISPHNAKVKTYMADMEPVIAMQPDALIMSDPGLIQ
                     MVREKWPEVPVHLSVQANTVNFATVKFWQSMGLSRVILSRELSLDEIEEIRQLCPEME
                     LEVFVHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHDASEDAAGVIRLQD
                     FDFKAEMEKSSLSGCGSLPRHPLAEKVYLIEEKGRPGELLPIMEDEHGTYIMNSKDLR
                     AIEHVERLVKMGVDSLKIEGRTKSRYYVARTCQNYRKAIDDAVAGRPFDWTLLGDLEN
                     LANRGYTDGFYQRHHTAEHQNYKQGYSSSNRSLYVGDIVAYDTEKGLADIEARNKFGV
                     GDRLQVIHPTGNQDVLVETMFNKKGEPIQEASGSGYFVRLPIAANDLSNAMVVKYLS"
     gene            complement(12626..13045)
                     /locus_tag="PKL42_02900"
     CDS             complement(12626..13045)
                     /locus_tag="PKL42_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNU66050.1"
                     /translation="MKSYRKELWFNLPARMAFKNITGEIRECLRESGVKEGLVLVNAM
                     HITASVFINDDERGLHHDYGIWLEKLAPHEPVSGYRHNDTGEDNADAHMKRQVMGREV
                     VCAVTNGQLDFGPWEQIFYGEFDGKRNKRVLVKIIGE"
     gene            complement(13158..13931)
                     /locus_tag="PKL42_02905"
     CDS             complement(13158..13931)
                     /locus_tag="PKL42_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HNU66051.1"
                     /translation="MVAAGLSVAFLSNAFAEADPNLWPVMKEAFFAKREMQEVDFIKI
                     DAPKRAESGAQVPVTYSIDNAAANGVKIVKLYSFVDSNPIPLTATYNLSDRLGDFQLS
                     TRIRFETDGYVRLVGETADGKLYLGSRVIRAAGGCGGMVENNEAEIRAAAGKIKMNVE
                     PPAKFGTPTAATFNIKHIMRTGLQRDLVSQGYVPAFYINKTEFKYNGKTLMDVDVGVG
                     TAEDPYFKFNFVPDAPGKLEVTATDNEGKSFTHEVDVKG"
     gene            complement(14198..14584)
                     /locus_tag="PKL42_02910"
     CDS             complement(14198..14584)
                     /locus_tag="PKL42_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNU66052.1"
                     /translation="MPVTQLNHVNLRTPFETMLKLKDFYCDVVGLKVGPREGFTSRGF
                     WLYIGDTHVMHLAEYRGEGAPLTNVLTTIDHISFTCTDMPAMEAHLKAHNIDYSIRDL
                     PVLNVKQINFKDPVGNGVELFFYMST"
     gene            complement(14640..15215)
                     /locus_tag="PKL42_02915"
     CDS             complement(14640..15215)
                     /locus_tag="PKL42_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66053.1"
                     /translation="MKKQYITILTATTLALSLASCSHVSPRYNASATNVEEIRQITKD
                     ISAKISTGEFTATEIGKKSIVCRAAGPVGPSDNKTFDQFIKDAIADEFKIAGIYDEKS
                     NVKLKGNLDSIDFSSSIGAGKWMMKMTFSSQEVEPFVVENTYSFSTNFIADIACEQVS
                     QALPAATQDFIKKLIQHPSFRNILTQKVAIN"
     gene            complement(15370..>16924)
                     /gene="aspS"
                     /locus_tag="PKL42_02920"
     CDS             complement(15370..>16924)
                     /gene="aspS"
                     /locus_tag="PKL42_02920"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNU66054.1"
                     /translation="RPEGTVNPNIPTGEVEMLATEIEILNASLTPPFMLDDDNLTETV
                     RLQHRYIDLRRPAMQKNLMLRYKVAKNLRDYLDDNGFIEVETPMLTRSTPEGARDYLV
                     PSRVHAGQFFALPQSPQLFKQLLMVSGFDRYFQITKCFRDEDLRADRQPEFTQVDIET
                     SFMSENDIMDIVEEMIRQMLKKVQNVDLPAKFPRMPFAEAMNKYGSDKPDMRVTLEIT
                     ELSDVMKDVDFKVFAGAANAEGGRVAALRVPNGAAISRSEIDAYTEFVKIYGAKGLAY
                     IKINDTTKLNEEGLQSPIVKNIHVKALQAIIDRTGAQNGDIVFFGADKTKVVNEALGA
                     LRLKVGHDKGHVDGRAWAPLWVVDFPMFEHDEENDRWVALHHPFTAPKDGHEDLLTSN
                     PGAALSKAYDMVLNGWEVGGGSVRIHKQEVQSKVFDALKISKEEAQEKFGFLLDALQY
                     GAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKTQRAQCLMTNAPNEVDEKQLRELHI
                     RVRTPNSVA"
BASE COUNT         4621 a         4168 c         3480 g         4655 t
ORIGIN      
        1 gttgccgttt accgattgcc aaagctcacc agtcgacaaa tttgccggaa cactcaaacc
       61 aaaatgctga taggccaagc tggtagccac gcgcccgcgt ggtgtgcgca ttaaatagcc
      121 ttgctgaatt aaatacggct cgagtacgtc ttcaatcgtg tcgcgctctt cgccaatcgc
      181 tgcggccaaa ttatccaagc caacaggccc gccgccaaat ttttctagca ccgcctgcag
      241 cagttttcta tccatgacat caaagccaag cttatcaaca tcgagcattt tcagggcggc
      301 atcggcaatt tctgcgctga ccttgccatt ggctttaact tgcgcgtaat cacgtacgcg
      361 gcgtagtaaa cggttggcta tgcgcggtgt accgcgagaa cgtttggcaa tttcaagcgc
      421 gccggtatca accaactcta cctctagcag cccggcagag cgttgcacaa tgcgacttaa
      481 ttcttcagcc gtataaaact ctaaacgcga aacaatgcca aaacggtcac gcagcgggtt
      541 agtgagcatg ccggcgcgcg tggtggcgcc caccaaggta aatggcggca aatctagccg
      601 aacactgcgc gcggcggggc cttcgcctat catgatatcc agacgataat cttccatcgc
      661 tggatataaa atctcttcta cgacgggcga caagcggtga atttcatcaa taaatagtac
      721 atcattgggt tctaaattgg tgagtaacgc tgctaagtca cccgcgcgct ctagcactgg
      781 gcctgaagtt tggcgcatat tcacgcccat ttctttggca ataatatgtg cgagtgtggt
      841 tttacccagg cctggcgggc caaacaagag cacgtggtct aacggttcac tgcggccgcg
      901 agccgcattg ataaaaattt ctaactgacc gcgcgctttt tcctggccga cgtattcatc
      961 taatagtttg gggcgaagtg cgcgctccag cgcttcttca cgcgggcttt cggtttctgg
     1021 agctatcagg cggtcagttt ctatcatgat ggtgctttcg ctgatgggtg ttgtgcgcga
     1081 accaatgcat tactaacaat gcatcattag cgcggatgta attcagcata ttgttgaatc
     1141 aattctttca cagtaatcgg caagccaaga tgcagccctt ggccatagtg cacgccgata
     1201 atttctagct gtttcaatat tgcatcactt tccacaaatt cagcaaccgt tttgatgttc
     1261 atcgattctg ccacattgtg gatggaacgc accatggcat gatcaatccg gttttgatga
     1321 atctctttga caaaagcgcc atcaatttta aggtaatcaa catcaaaatt ctttaaatac
     1381 ataaatgaag acatgccact gccaaagtca tctaaagcaa atttgcagcc aatttcacgc
     1441 agtgagttaa acaataaaat ggtattttta aggtttacaa tggccgccgt ctcggtaatt
     1501 tcaaagcata tcgaggcggg cggaatagcg tatttagcaa actgttcgcg aataaacggc
     1561 aacagacttt ggtcacccaa tgacgtgccc gataaattga ttgaaaagat gtaattgcag
     1621 tgttcgtaat caatatcaag cagttttcga taggcaataa aagcattttt aatcacccag
     1681 cggtcaatat taggcatcag atgatagcgc tcggctgagg gaataaacgt cgttggcaaa
     1741 ataatcttgc cgtctaaatc tacttttctg agcaaaatct catagtgatg ttgctgtgga
     1801 atggccggca tattaggtaa atgcggtaaa ttcggcgtga gcggtacaat cgcctgacag
     1861 tacagcataa actgatcatc atcgatttca tccgtgatgt cggcaatcgc ttctactgca
     1921 tttttctgct cgaccacttc aacatcgcct gcgttaaata gcaccgtttg attacgtccc
     1981 gcctttttcg cggccataca cgccatatct gccgcgtgta ccacttcgct aacactgtca
     2041 aaatggtcat caatcaccac cataccaatg ctcacgccaa tattaaagat tttgtcgtca
     2101 tggctataac gaaaatcgcg aataatgcgt tgaattgcac tgcaaatttg gtcggcttct
     2161 ttctgcgtac agttctccaa cagcaggcca aactcatccc cgcctaaacg ggctaatgtg
     2221 tctctttcac gcatgcggat ggccagtaaa acactcactt gtttaagtag ttcatccccc
     2281 gcgatatgcc cactcgtgtc attcactaac ttgaattgat ctaaatccag aaagcaaaac
     2341 acgtgctttg cattattctc tttagcactc ttgaggcagc gctctaagcg cacttcaaat
     2401 tctcgacggt taataagccc agttaaatca tcatgttgcg cttgatggcg tagcatagag
     2461 gtggcatcgt taatcttatc ttgcatcaca tcataggcgt gcttcaagct cgccgacatt
     2521 gaattaaagc ccttttgtaa ggtttttaat tcaccgctgg agttttctac caaggtttgt
     2581 gacaacacac cttcgcgcac tttattgact gcacgggcaa tctcctgaat aggctgtgta
     2641 atatttcggc cgatacgcca agctagccag ccactgagca acagccccaa taaagcaatt
     2701 gatgcaattt ttacgacaaa tactaactta taactctgca gtccttgatt acttagttcc
     2761 acataaatct gaccgatttt ttcaggtgaa aaatcttttt tcagttgttg cgtattttct
     2821 tctagggacg aaaaatcatc aatctcgact aaacttcgat gaataggcgc agtaaaaatg
     2881 atgccgcgtg cttgtttgta ctcaggcaaa ttttcctgca tgattttgct aatcagcgtt
     2941 tcaggaatcg cctgtccact gtaggccagc actaatcctt gattatcaac gaccatcacc
     3001 gttttaagat cttgctcatg atcaagcgct tgctgcacaa gtttttgcag catttgtcga
     3061 tttccagaaa tcaccccgta ttcagcagca ggggcaagtt gagtagcgat gatttttccg
     3121 cggtcaacca gcgattgatt caatatgcca aatatatttg aaattatgta gccgattaaa
     3181 ataatcgcaa acagcagggc aggcactgtg cctaaaaaaa tcacccttgc tttaatgccg
     3241 atattattca ttttgcaaat tatcagtggc tattaattct tgaaaaattg ccacatcttt
     3301 gttcacttca atattgagca aacgcgccac ttgccgattg acgtacacag aaaaatattt
     3361 tggcgcttgc ggcaatggca aggcgctttc tgcattttgc gattgctgcg caatttccgc
     3421 agcctgcttg gccaaatgcc ggctagagct atataacgcc gccagtgcgc ccgccttcgc
     3481 ataagagagt gaataacaaa gcactggctt cttaaactga taactagtca tcaaaataga
     3541 ttgcgccgtg tcgggtttat aaacagctgt atctggtatc gccagcaacg catcattcgc
     3601 ctctaacaaa gttttaagct ccggtatcaa catcgatgga tcatttaagt gcgttaactg
     3661 cacattgagt ccttttttat cagcctgtgt tttcaactgt tccgcatatg tttgcgagtt
     3721 gccattgact aagctgccaa tattttttgc cgccggtagc accaattgaa taaaatttat
     3781 ttggcgcgca taaggctgat tttgcataat catgctcata ttactcgcat cacgccgact
     3841 tttttctaat atctgctcat aggctaatga cggcgtatgc actgccaaaa ccggcgttgt
     3901 atgcgataac ttgctggcgg cctcaagcgc cttttcaccc aacgcaatca ccaattcact
     3961 attttcagcc accacgagcc ttttgcgtgc tggcaactca atcactttta gattcagtgt
     4021 attattttca ttgctagcta gctctgtctt aaactgctca acaaacgcca gattcgcttc
     4081 agttttgcta ctcatgacaa tcgtcacgcc tgtataagct agcgccacct cagaaaatag
     4141 gcacattacc gcaaaaaagc agcaggcaaa ccgcattgaa aatacgctat ttttattttt
     4201 atattgactc acatttaacc cacaaaacaa actgattgat tacgcaaaaa catcacaaaa
     4261 accgcaataa aattcccgag acaaaaatac tgctctgcga gcctttattt acaaggcttc
     4321 cagagcattc aaaaaggttg tgcgtattat acccgtttga aattgtaaat taatgtatta
     4381 cgccaagttt atacatgcgg tttttttgtt ctaattttag cccaaagttt gcgaattaaa
     4441 atcagtgctg gaatgccaaa aataagccac ggcaaaattg ttacaaaaaa agtaatcacg
     4501 ctcgctaaac tttccatcat tacgtggcca gcatttttaa aagcttgtgc aacgggtgaa
     4561 aaaaagcctt gctcagtaat gccttgcttg gcagtaaaat caatattaat cgccaccaat
     4621 tcggtttctt gcgctagcac tttgcgcaca ccctgaatac catctaactc actttgcgtg
     4681 ttgactaact cacgctgcac ttcgattaaa tctttaaatt tggcagattt atcgctcagc
     4741 attgcgcgca aattatcacg aaactcggtc aggtttttaa tgcgcgcctc tgcatcaatc
     4801 accgcatcgg ttttatcttc gctgctacgg tgatgctgta agacttctcc acttttagct
     4861 aggcccgcta agaacaccgc tactgatctt ggtggaatac gcgctgacag gcttgccgat
     4921 ggcgggctat aagctgtttc acgattataa ttggccgaca aaatttggca cttgagctgc
     4981 tgacaatgcg caacggtggc atcaaaattt gcttgcattt gttctgttgg cgtttccacc
     5041 accaaatgat ggcgtaatgc gataaagcgt ttaacctcgc tttgagcagt agcaggctct
     5101 gtaagttctg taagtgactg ctcaccttca cccgcaacgg ccactgaatc agcactggcc
     5161 atttttgtac ttgccatctc ggcgggcgct ggtgccatcg ctaccgttgg cacaggtggc
     5221 tgttgctggc cacacgccgc caaaaacaca gctaggctaa caataaaaat tagatatctt
     5281 ttcatcactc atcctcacat tgcttcaatg ttttgttgtt attttgaaag gtttttcaac
     5341 gcctgcttaa tcccatcggt cacgctcact tctttgtcta gcaatttaac ggcggccagc
     5401 gcctcgcgct cattgtagcc caaagccacc agtgcattga ggatgtcatg cgtggcagac
     5461 tttgcctgat tggcggtttg cgccaagcct gccacactga atttgtcttt gagttcgagc
     5521 agtaaacgct ccgcagtttt tttgccaatg ccgggaatgc gcgtgagcat gccgacctct
     5581 tgcatgctca ccgcctgaat caaatcatca atacttacgc cacttaaaat agacaacgcc
     5641 gatttagcac caatgccatt gactttaagc aattgtttaa acgtactttt ttcttgttcg
     5701 ctaccaaaac catacagcag ttgcgcgtct tcacgcacca caaaataagt cagtaaattc
     5761 actttactgc ctaattctgg cagattataa aatgtgctca tcggcacctc aacttcataa
     5821 cccacaccat tgcaatcaat cacgatttgc ggaggagatt tttcaatcag tattccatta
     5881 agtctggcaa tcatcttgca ttccaaaata ataataaagt taatagtaag gcatggccca
     5941 gcatggcaag caaggttaac tgaatcgctg ggcgtaattt tgcaggttca gcataatgtt
     6001 gttgcaaaag ccaagccgcg cgcaatgcaa atagtagcgg caacgctgac catagtgtca
     6061 ttgctggcag cctttgtaac aacgccatcg cgagcagcca aacacatgcg agcaatacca
     6121 atattgggta aatatttgcg gctgttgtca gtggcaagag cacaacccaa tgatgcttgc
     6181 cggccagctt atcggcttta taatcaggaa actgattgat aaaaagcaag ttcgtcacca
     6241 gcaatgcata gggaaacccc acaaacacag gctgccaaga aaagctgatg cgctgcacaa
     6301 aatcggcccc aattaccaca cctaaaaaac ccaataacac gcacacctcg cctaaaccgc
     6361 ggctatttaa gcttaacggt gtggccgaat acgcccagcc aatgaataga ccaacgccac
     6421 caattagcaa aagacctttg ccgacttcag ccaccaacca tagcccaccc aacatggcaa
     6481 cgagcaacaa cccatagcca aatcctgcag tttgttgcgg agtaaacagg ccattctgaa
     6541 taaaacgact gccacccgta aatggataaa cgcgctcggt attaaaagca tctgcaccac
     6601 ttaacgcatc aaaataatcg ttaaggacgt tcactgcggc atgcacaact aacgccagca
     6661 gtattgtcgc aattgcaaaa gccacattca gattaacgcc gcctagtttt gcacttgcca
     6721 aacccagcaa acacgcggcc aaagcggcta acaaaaacgc tggccttgta gcaaaaaaat
     6781 acttggtaat attattggcg taagtgccaa tggcaggttc aagcaacagt ttcgcattgt
     6841 ttgtcaccca atcagcctcc cgcccttgac gcgaaaacca gccgtggcca acctaccaag
     6901 gccttgcccg ccgtgtgcgt ggcatatcgc acaagccagt gcatcggcag aatctggctt
     6961 gggtgtggct ggcaaattga gcaaacgttt caccatttct tgcacttgtt ctttttgtgc
     7021 gtgaccattc cccaccaccg actgcttcac ctgcaacgcg gtatattccg ccacaattaa
     7081 attgcgaatc actgccgcgc taatggccgc gccacgcgct tggccgagca gcagggtaga
     7141 ttgcgggttt acattcacaa acaccttttc aaccgccacc tgcgttggtt gataagtatc
     7201 aatcacttca aacaggccgt ctaaaataat ttttagccgt gctggcaatt cttctctatc
     7261 ggcctcgccc tcaacgacat tttttttgcc gcttgcagtt ttaatggtgc cgcttgccac
     7321 gtaaacgatt ttttcaccaa ccttttcaat gacaccaaac ccggtcaagc gcaggccggg
     7381 gtcaatacca aggatacgcg tggtaactaa cgccacttat accaacccta ttacggttaa
     7441 gtttaagggc ctttttttac aaagtgtttg tgtgaaatgc atgcgtaaat gattggtttt
     7501 gggttaactt aagacggtaa atacagcaca aattataagc cattcacttg cttacttact
     7561 gatttaattt gcttcttttg ggcattacct tacaatcaag cttagtaaaa taattatcca
     7621 atcatgctta agacccaaca catgctcaca aaattactca ccgcaccatt taagcttttt
     7681 gcaccactca aaaagtttgg ctatcgcatg ttttttgcca ctggcgtggg tatcgcggta
     7741 ttatttatcg ccttcagttt gctattagac aaacaaagcg tcgcggtact gcttgccgcc
     7801 attggtgctg gcttaggcgc gttgatcatc agcttaacat tgccaaaact actggtgtct
     7861 gccgacgtgg agcggcaaaa acaacttgcc agcgaaatgc aaaaagtgct caaagacaaa
     7921 gccaaactgg tgattgaact ggagcgcatt aaatcacagc aaatgcagtt tcagcagatt
     7981 caggccgtgc taaaactcac gctacttgaa gtggatttat cgctcaccga ttttaagcaa
     8041 gaagatttag gcgagcgcga aaaaacgctg ggtggtaaaa cctcaacgca atatatcggc
     8101 gtgattcgta aaaaagccaa agccatgctg ggccttgatt tagcgcacat ccgtgtgcag
     8161 caaattggca aagaactgct ggtggacaaa ctgcgcgcgc aataccaagg cgtttctgcc
     8221 aatactgaag aatggctttt tcgccaattg cagattcatg aaaataattt agtcatgccc
     8281 aatcgcatca acatcaatct tgatagcaac gagctcatgc acgccagcga tgcgcaccgt
     8341 gaagatttaa accaacgcat taacaacggc gttgaactca aagcctttga ccacgccatt
     8401 gaaaaaatgg gcgaacaatg gctaaaactc atgtttgcgc caatgggctt aaccgtgcgc
     8461 gtggcacaaa tcaatgaaga agacagtgtg ttgctactcg attacatcaa aagtacgcag
     8521 ctgcaccttg aacaagcgca acatttatta gaagaaaata ctgcgctgat ttagaaaaaa
     8581 tagttggctt aatccacccg agataaaaac gctgccctgt gagccttata aattaaggct
     8641 tgcagggcag cgttaaagta gatgcataca aactgcttaa atattaagca gtttgtagtt
     8701 ataagcccac gtaaaattgg ctaactttta ttcttcaatc aacgccgtgg tataaacatc
     8761 ctgcacgtca tctaagtctt ccagcgcatc cagaattttt tgcattttca ctgcatcatc
     8821 cccggtaaac tccacttcat tcagggcttt catggtcact tctgcgagca cagctttgag
     8881 acctgccgct tctagcgctt ctttaaccgc cacaaaatcc gctggcgggg taatcacttc
     8941 aatactgcca tcatcgtttg tcacaatatc ttcagcacct gcctcaagtg caacttccat
     9001 gactttatct tcgtcggttc ctggctcaaa aactaaattg ccacagtgtt taaacataaa
     9061 cgctacactg ccgtcagtgc ctaaattacc gccaaatttg gtcaaggcat gacgcacatc
     9121 ggccaccgcg cgctgtttgt tgtcggtgag gcagtcaata ataatcgccg cgccattaat
     9181 gccgtagcct tcatagcgaa tctcttcgta gttcacgcct tcaagctcgc ccgtgccttt
     9241 tttgatggcg cggataatgt tgtctgacgg catattttct tcttttgcct cggccacggc
     9301 gctacgcaag cgcgggttca tgtcggcatc gcccccgcct aggcgcgcgg ctacggtgat
     9361 ttctttaatg attttggtaa agattttgcc gcgttttgcg tcctgacgcc ctttgcggtg
     9421 ttgaatgttt gcccacttac tatgacctgc catgctacat tgtccttgaa actgaatgat
     9481 aaagaccacc attttagcat gtaggagcgc aatttattgc gcgattgccg gcgcggagtt
     9541 gcaacgcttt tgcgcaatac cacaggcata agtactgcta tcatgcaagc atgaggcatt
     9601 actaataaat gcgctcctac aagttactct tggccttgtg ctgcgtccac ttgcgcatgt
     9661 ttgcgtatgc taatcgcaga aataatcacc aaagccaacc caataaaact catgccaatc
     9721 agttcttgtt tgcctggcac ttcattaagt tgcaaccatg ctgaaattac gccaatgacg
     9781 ggggctagca tacttgccat gctggcgatg ccagcattta agcgctgtag tgcgtacaac
     9841 cataaaagcc acgccacaac accgcttaaa atcacattaa atagcacggc ggctataaac
     9901 gtattggtcc attgaattgg cggcgcatct aacatcagcg cgtaaatcac cagcggaatt
     9961 gagcctagca gcattggcca tgccgtgata ttaagcaagt ctaaatgcgg tgcgcgtttg
    10021 tgtaattttt ttgaaataat cgccgaaatc gcccaaaaaa cacctgaaca tacagccaaa
    10081 aacatactga aagcatcggc tttaatgtgg agtggatcaa atatcaaaat aatgcccaat
    10141 aaagctgaaa ccacggccaa ccattgccag ccttgcactt tttcgcccag catgggccac
    10201 gcaaaaagca ttacccaaaa gggcatggtg tagcttaaca ccgctgtttt gcccgcaccg
    10261 ccgatggaaa cggcccaaat aatcagcccc gtaaagccga tggtttgtag aaaacccagc
    10321 accaacatgg tgggaaattc tttcaccatc attgcacgtt ttgtaaacgc cataatgata
    10381 aacaaaatga tcgcgccact aaatgtgcgc atggcggcaa actgaaaagg gttggcgtat
    10441 tgcactgcaa gcttcatcac cacccagtta tagccccaaa tcagcgtgag tataaacagc
    10501 gcaaccaatg ccttaatggt gatatttttt tgcaaaatga gtgtctttaa gttgagagta
    10561 ttgctattgc cattataaag cgtatatttt taaatcgctt gcacaaaaaa aggggagagt
    10621 catgaaaaca ttgcaacaac tactcgatag taaaaaacat aaagaagtga tttcagttgc
    10681 accgcatcga cccgtgtttg atgcgctggt ggtgatggca gaatatcgaa tcggtgcgct
    10741 ggtggtgctt caaggtgaaa agttagtcgg tattttttca gagcgcgatt atgcgcgtga
    10801 ggtggtgctc aaaggtcggt cttcaaaaac cacgcaaatc agtgatgtga tgaccgacaa
    10861 agtgctgtca gccaaaccat ctgacaccgt tgaacaggcc atgtccatta tgtctgaaaa
    10921 gcgcattcgc cacttgcctg tggttgaaaa taaccaagtc gttggcatgc tctcaattgg
    10981 cgatttggtc aaagaaacca ttgattatca gcagcgttta attaagcagt tagaaagcta
    11041 tattcgaggc taaaaacaac acaacctttt tgactgccct gcaagcctta taaataaagg
    11101 ctcgcagggc ataaaaattt tctcgggcga ttatttggct taatttagcg cttttttatg
    11161 acaaatattt caccaccatc gcattactta aatcgtttgc cgctatgggt aggcgcacaa
    11221 aataaccgct accagaagcc tcttgaattg gctcaccttt tttattaaac atggtttcaa
    11281 ccaacacatc ttggttgccg gttggatgga tgacttgcaa acggtcgccc acgccaaatt
    11341 tattgcgcgc ttcaatatcc gccaggcctt tttcagtgtc gtacgccaca atatcgccca
    11401 catacaaact gcggtttgag cttgaatagc cttgtttgta gttttgatgc tcggctgtgt
    11461 gatggcgctg ataaaaacca tcggtgtaac cacggttcgc caagttttct aaatcgccca
    11521 gtaatgtcca atcaaacgga cgacccgcta ccgcatcatc aatggctttg cgataatttt
    11581 ggcaagtacg cgccacgtaa tagcgtgatt ttgtacggcc ttcaattttc agtgaatcca
    11641 cacccatctt caccaaacgc tctacgtgct caatggcgcg caaatctttg ctgttcataa
    11701 tgtaagtgcc gtgttcatct tccataatcg gcaacaactc acccgggcgg cctttttctt
    11761 caatcaaata gactttttca gccaatggat gacgcggtaa actgccgcag cccgaaagcg
    11821 aagatttttc catttctgct ttaaagtcaa aatcttgcaa gcgaatcaca cctgccgcat
    11881 cctcgctcgc atcgtgcact ttgtaatccc aacggcagct attggtacaa gtgccttgat
    11941 tcgggtcgcg atgattaaaa taacctgaaa gcaagcagcg gcctgaataa gcaatgcaga
    12001 gcgcgccgtg cacaaatact tcgagctcca tttcggggca aagctgacga atttcttcaa
    12061 tttcatccag tgacaattcg cgcgataaaa tcacgcgact taaacccatg ctctgccaaa
    12121 atttcaccgt ggcaaaattt accgtattgg cttgtactga aaggtgcacc ggcacctctg
    12181 gccatttttc acgcaccatt tgaataaggc cggggtcaga cataatcagc gcatctggct
    12241 gcatggcaat cacgggctcc atatctgcca tgtaagtttt tactttggcg ttatgcggtg
    12301 aaatattgct cgccacaaaa aactttttgc cacgcgcgtg cgcctcttta ataccaatct
    12361 caagattggc catggtgaaa tcgttattac gcgcccgcaa actgtagcgc ggctgccccg
    12421 cataaatcgc atctgcgcca taatcaaacg cggtgcgcat acggtctaaa tcgcccgctg
    12481 gggctaacaa ttcaggggtt ttcatatcgt tactcctaat cctagctacc cgttcgtggt
    12541 gagcttgtcg aaccatgaac ggcttattaa attttatttt tttgtcctga gcttatcgag
    12601 ggtcaaacgg ctattaaaaa caagtttatt caccaataat tttcacaagc acacgcttat
    12661 tacgcttacc atcaaactca ccgtaaaaaa tctgttccca cgggccaaaa tcgagctggc
    12721 catttgtcac tgcgcacacc acttcacgtc ccatcacttg gcgcttcatg tgggcatcgg
    12781 cattatcttc acccgtatca ttatggcgat aaccgctcac tggctcatgt ggcgcaagtt
    12841 tttctaacca aatgccgtag tcatgatgca aaccgcgctc atcatcatta ataaacacgc
    12901 tcgcggtaat gtgcatggcg ttcaccaaca ccaaaccttc tttcacgccg ctttcacgca
    12961 aacattcgcg aatttctcca gtaatgtttt taaaagccat gcgcgctggc agattaaacc
    13021 acaattcttt acggtaactt ttcatgttta ctcccttatt actttttaaa agttgctaac
    13081 gcacatttta acatcttgta tcgtaaacaa aaaaataaaa aaggcctccg tagaggcctt
    13141 tttagcaaac cgtattacta accttttacg tctacttcgt gggtaaatga tttgccttcg
    13201 ttatcagtgg ctgtcacttc taatttaccc ggtgcatctg gcacgaagtt aaatttaaag
    13261 taagggtctt ccgccgtgcc aacccccaca tcgacatcca ttaaagtttt gccattatat
    13321 ttaaattcgg ttttattgat ataaaacgct ggcacataac cttgcgacac taaatcacgt
    13381 tgcaagcctg tgcgcataat atgcttgata ttaaatgttg cggctgttgg cgtgccaaat
    13441 tttgcaggcg gctccacatt cattttaatt ttacccgcgg ccgcgcgaat ttctgcctcg
    13501 ttattttcaa ccatgccgcc acagccaccc gctgcgcgaa tcacacgaga gcctaaatac
    13561 aatttgccat ccgctgtttc acccaccaaa cgtacgtacc catctgtttc aaagcgaatg
    13621 cgggttgata attgaaaatc tcccaaccta tcactaaggt tataggttgc ggtgagtgga
    13681 atagggtttg agtccacaaa agaatatagc ttgacgattt ttacgccgtt tgccgcagcg
    13741 ttatcaatgc tataagtcac gggtacttgt gcgccacttt cagcacgttt tggtgcgtca
    13801 attttaataa aatcaacttc ttgcatttca cgttttgcaa aaaacgcttc tttcatcacg
    13861 ggccataaat ttgggtcggc ttcagcaaat gcattactta aaaaagcaac agacaaacct
    13921 gccgccacca tccatcggcc tagaccgctc gttaggccaa atttaagttg agattgtgtg
    13981 ttcattttat ccactccaaa atattattta gttattttat aggcgttttt tgcttacaca
    14041 ttgagacagt aagcttcacc acgtagttcg cattctaagg gaatatacgc aaaggtatgt
    14101 gataccagtc acacagacca aaatgatagc cgcttatcta aaaatgtgcg cattagatag
    14161 tgcaagaacg taaaacagaa tgttgcgttg acgccactca agtggacata taaaagaaca
    14221 actcaacgcc attacccaca gggtctttaa aattaatttg cttgacattc aacaccggta
    14281 aatcacgaat cgaataatca atattatgcg cttttaagtg cgcctccata gccggcatat
    14341 cggtgcaggt aaacgaaatg tggtcaatgg tggtgagtac attggtaagc ggcgcgcctt
    14401 caccgcgata ctccgccaaa tgcatcacgt gcgtatcgcc gatgtagagc caaaatccac
    14461 ggctggtgaa accctcacgc ggcccaacct ttaagccgac tacatcgcag tagaagtctt
    14521 tgagtttcag catcgtttca aaaggcgtgc gaaggttgac gtggtttagc tgggtgactg
    14581 gcatgatttc aatttaaaac tttccttaac tggcattttt aaatctatcc atttatcaat
    14641 cagttaatag caactttttg tgtcaaaata tttctaaaac tcggatgttg aataagtttc
    14701 ttaataaagt cttgagttgc tgcgggcagt gcctgagaga cctgctcaca cgcaatatct
    14761 gcaataaagt ttgttgaaaa ggagtaagta ttttcgacta caaatggctc tacttcttga
    14821 ctagaaaatg tcatcttcat catccatttc ccagcgccta tgctagaact aaaatcaata
    14881 gaatcgagat ttccctttaa tttcacatta gatttctcat catatattcc cgcaatctta
    14941 aattcatccg ctattgcatc tttaataaat tgatcaaaag ttttgttatc tgacgggcca
    15001 accggtcccg ctgcacggca tacaatagac tttttgccta tttctgtcgc ggtaaactct
    15061 cctgtactta tctttgcgga aatatccttg gtaatctggc gaatttcttc aacattggta
    15121 gcggaggcat tgtatctagg agatacatga gaacatgagg ccaaagataa agctaaagtc
    15181 gttgcagtta aaattgttat gtattgcttt ttcatttttt ccccgtttaa tttatttcga
    15241 aatatagttt tatcagtcta ttcacagttc agtatttcaa actgggtttg gctcacatat
    15301 taagcatatt aactcacgta attgtatgac caagtgaatg tagtgaagtt attgcaaagg
    15361 gagtgcattt taagccactg aattgggcgt acgaacgcga atatgcaatt cacgtagctg
    15421 cttctcatcc acttcgttcg gtgcattggt cattaagcac tgcgcgcgtt gggttttagg
    15481 gaaggcaatt acatcacgaa ttgattcagc acctgtcatc aacgtcacca aacggtctaa
    15541 accaaacgcc aagccgccgt gcggaggggc accatattgc aatgcatcga gcaagaagcc
    15601 aaatttttct tgcgcttctt ccttgctaat ttttagcgca tcaaacactt tcgattgcac
    15661 ttcttgttta tgaatacgca ctgagccacc gcccacttcc cagccgttga gtaccatatc
    15721 ataggctttt gaaagcgctg cacctgggtt gctggtaagc aaatcttcgt ggccatcttt
    15781 tggtgctgtg aacggatggt gtaaggccac ccagcggtca ttctcttcat catgttcaaa
    15841 catagggaag tccaccaccc acaatggcgc ccaagcgcga ccatctacat ggcctttatc
    15901 gtggcccact tttagacgta atgcacctag cgcttcattc accactttgg ttttatctgc
    15961 accaaagaat acgatgtcac cattttgtgc gccggtgcgg tcaataatgg cttgcagggc
    16021 tttcacatga atatttttaa cgattgggct ttgtaggcct tcttcgttca attttgtggt
    16081 gtcgttaatt ttgatatacg ccagcccttt ggcgccgtag atttttacaa attcggtata
    16141 agcatcaatc tcgctacgag aaattgccgc accatttggc acgcgcaagg ccgccacacg
    16201 gccaccttca gcattcgccg cacccgcaaa cactttaaaa tctacgtctt tcatcacatc
    16261 cgatagctcg gtaatttcaa gggtgacgcg catatcaggc ttatctgagc catatttatt
    16321 catagcttct gcaaatggca tacgcgggaa ttttgcgggc aaatcgacat tttgtacttt
    16381 tttgagcatc tggcgaatca tttcctccac gatgtccatg atgtcgttct cgctcataaa
    16441 cgatgtttca atatcaactt gggtgaattc cggctgacgg tcagcacgca agtcttcatc
    16501 gcggaagcat ttggtaattt ggaagtaacg gtcaaaacct gacaccatta acaattgctt
    16561 aaacagttgt ggtgactgtg gcaacgcaaa gaactggccc gcatgcacac ggcttggcac
    16621 taggtaatca cgcgcacctt caggcgtact gcgtgtgagc atcggtgttt caacttcaat
    16681 aaagccgtta tcatctaagt agtcacgtag gtttttagcc actttgtagc gtagcatgag
    16741 gtttttttgc atggctggac gacgcaaatc aatgtagcga tgctgtaagc gcacagtttc
    16801 agtcaggttg tcgtcatcca acataaaggg cggggtgagt gatgcattga gaatctcaat
    16861 ctcggtggcc agcatttcca cttcacctgt cggaatgttt gggttaacgg tgccttctgg
    16921 gcgc
//
LOCUS       DAOQSI010000024        16655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_2552_length_16655_cov_5.525602, whole genome shotgun sequence.
ACCESSION   DAOQSI010000024 DAOQSI010000000
VERSION     DAOQSI010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 16655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16655
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2552_length_16655_cov_5.525602"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PKL42_02925"
     CDS             <1..517
                     /locus_tag="PKL42_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66055.1"
                     /translation="AETAQNNDAQPNTDLDDYDVLVAADFLSCYVTFKAIQAAERSPS
                     AERNENFDILSVYQAYALLAYAFFTRPLGAEDITPNFQTAQITIAKTLFAGLPEPELI
                     EIIESGLHKFQLIADAEVEHWTEFRENLDKLTVAFIVAGTDDESPHGKEELFPLFGQL
                     LSQLCEAFENV"
     gene            772..2238
                     /locus_tag="PKL42_02930"
     CDS             772..2238
                     /locus_tag="PKL42_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HNU66056.1"
                     /translation="MSTPILIAKNQETSNFLLPKMANRHGLIAGATGTGKTVTLQSLA
                     ESFSAQGVPVFMADVKGDLAGMSQIGGGNAKVEARVQQLALTDFSYKKSPVAFWDVFG
                     VKGHPVRTTIAEMGPLLLARLLNLNEVQGGVLNSVFKIADDNGWLLLDLKDLRAMMQH
                     AAENSATYQAQYGNISAASVGAVQRSLLELETQGADQLFGEPALNLDDLMQTDADGRG
                     IVNILASDKLFNSPRIYATLLLWLLSELFEKLPEVGDLEKPKLVFFFDEAHLLFNDAP
                     QALLTKIEQVVRLIRSKGVGVYFVTQNPLDIPDAVLGQLGNRVQHALRAFTPRDQKAV
                     NAAAETFRSNPKVNVATAITELGVGEALVSLLDEKGIPTPVERTFVCPPSSRIGPITD
                     GERLDVMKTSLVAGAYEKTVDRESAYEILKTRATQSATEAKADEGFSWGDILGGKKTS
                     RSDTILESAAKSAARAVGSQLGRSIIRGVLGSILGKGR"
     gene            2267..2839
                     /locus_tag="PKL42_02935"
     CDS             2267..2839
                     /locus_tag="PKL42_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNU66057.1"
                     /translation="MISILEAGFAEQTINKSRFIAAALPCADEREIGAALRAFAAQHP
                     TAHHLAYGFRIKTTDGIIPRFSDAGEPSGTAGMPILKHIEGRDFINVCVAVIRYYGGI
                     NLGTGGLVRAYGGTAKLALDAAKTAEYVEMRSYPLTISYKQMDMLTNVLPKCNGTILS
                     KSFNAQVDLVLSLPVNEAENFLNRFKPYGS"
     gene            2939..3973
                     /locus_tag="PKL42_02940"
     CDS             2939..3973
                     /locus_tag="PKL42_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNU66058.1"
                     /translation="MINTSKKLPPNVYEIAAWVVAAIVLVFVLVFHLLPALLAGLLVY
                     ELVHMLTPQIAKRFPTKKPSNRAKLWAVGILVVIIVGLLSLAGAALVAFLRSDAGSVT
                     VLLAKMAQILEDSRKILPTWLLDHLPADAHTFREQASEWLRSHADELQVVGKEAGRAT
                     AHVLIGMIIGGMVAVRDAVRMDSRKPFARALATRGDMFGDAFRRIVFAQVRISAINTT
                     FTAIYLAVVLPLMGIHLPLIKTMIAITFLVGLLPVIGNLISNTVIVVVSLSQSLGIAI
                     ASLAYLIIIHKFEYFLNARIVGGQIRANAWELLIAMLLMEAAFGLAGVVAAPIYYAYI
                     KSELRRRELV"
     gene            3990..4418
                     /locus_tag="PKL42_02945"
     CDS             3990..4418
                     /locus_tag="PKL42_02945"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HNU66059.1"
                     /translation="MMVSAYLLNSFLVAAAVLIHFEALNLLTRLIPHLPIRQRLRILF
                     GVFGALIAHVVEIWVFAFGYYFMIHQGGFGTLEGHFDQSLMDCSYFSFVSYTSLGFGD
                     IVAKGDIRFLAGLEALTGLVLITWTASFMFIEMQKFWKDH"
     gene            complement(4473..5141)
                     /locus_tag="PKL42_02950"
     CDS             complement(4473..5141)
                     /locus_tag="PKL42_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66060.1"
                     /translation="MQDQIDIFVSSLNQFWHQVASFFPKLLAVIIILFFGWLIAKGSR
                     IAVKRFLKWVKFDKFSGKSGLESYLQNDDYDVSLSGIISQIVFWLVILLFIITGANAL
                     GLTEVADMLKQLANYLPKIIVAIVVLIFGTLLGRFVNRLVFAWLHSIKFDGALKVSTS
                     AEYLVQIFAIFVALEQLDIGSQLVTALFIIMFGAIFLALAIAFGLGGKDYAAKIIEDR
                     QNQK"
     gene            complement(5449..6423)
                     /locus_tag="PKL42_02955"
     CDS             complement(5449..6423)
                     /locus_tag="PKL42_02955"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNU66061.1"
                     /translation="MSAATFQYRQLFFVVAGLVTALASAQTQALDLGKALEKAAKQKI
                     EEIKTDNSTNSEPQKTEAAASNASNIQATANADATSTPAESTPFNWKNPSRAEEVALG
                     REISGSVLGAAPLVNDAALQKYVNNVGRWVASQSERPDLPWKFGVIESNDLNAFAAPG
                     GYVLVTKGLYQKLQNEAQLASVLGHEIAHVVKKHQLKVLQKQQLLGYGAGKLGEMFAK
                     DDKVAKKVIDSGAEISARSLDKDAEFEADRMGMVLATRAGYDAFSLGEVLQTIGQTNK
                     TDNSVALLFKTHPHPDERLEKLGSSAGNKFDQVQNGKTLENRFYTLKN"
     gene            complement(6423..6929)
                     /locus_tag="PKL42_02960"
     CDS             complement(6423..6929)
                     /locus_tag="PKL42_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNU66062.1"
                     /translation="MKQFTKLHSKKWLLLALLLPAISIAAELGTALKADTLRVEPFSD
                     AKTKGTIAKNETVEILNKKGAWLHIKTQKNTGWVRIFSVKRGSASNTNQAKGVYDVAS
                     GRASTGKVISTTGIRGLSAEELKAAKFNEEEMKKLESYAVSAEDSQKFAKAGGLKSVQ
                     FAYLKGAK"
     gene            complement(6926..8647)
                     /locus_tag="PKL42_02965"
     CDS             complement(6926..8647)
                     /locus_tag="PKL42_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNU66063.1"
                     /translation="MTKLKLLLGQWLANMTRKLKNNFYIYLAALFTVLVLLDASVFGV
                     GLNMRDRAFDFMVKYRVIQPQADPDIIIVDINEASLHAFAKEFGSWPWSRQVMGEFVE
                     NIQAQKPKAIVFDVLFSDADIFRPEGDEYFNEVISSADNTFFPILRLDISQDRHSELK
                     FSQIPGLKFKQDSGNQDATFAAILPHFEGAIKANRLGTHNVYPDIDGIVREYRIYHDD
                     YGWFLPSLPLIVGDFSQMNSYPLPQNMLINWRGKPFSYQYVPFSDVFTDLTSKVKKRP
                     QNEFTNKIVIIGSTAPGLFDIKATAMDKQFPGVEILATAIDNVKNRDYLKVWRGKTPY
                     ILLSLLLIWLTTLAFYRSMDRDRFNTIFTSGQMGLLVLSYLAINFTNTYLDLTAPILW
                     AVAFFGIAKIYALANDRALQRWLTFGVKSGDSKTLALIMPILLESNDPLNHDALNDAL
                     LRKFKRQIELTSKTPSNIEVLHGTQGGIWGLFGDMVVVTWQFSDQKDEYVTAAKHDAQ
                     LLAKSLPHLSQALDLPTSTIIRYSLHEGQLENDSAAALASQWRALFAQAVIKLEQNDH
                     ASLKDLA"
     gene            8967..10289
                     /locus_tag="PKL42_02970"
     CDS             8967..10289
                     /locus_tag="PKL42_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNU66064.1"
                     /translation="MNMIFLQWIATLITLLLLAWLLWRSYQNKSTELLLQQLEDKHRA
                     MLLDLNDGLNKLGDRINAASQEGSERLKSAVATELQATREAMQALQLAQNNSLAQTRE
                     TVLEKLHTTLSEQGKSQQALINDTMLKATTTLTQSVESLGKIVDSRLEQIGGKVSERL
                     DEGFKKTNQTFIDVMARLATIDEAQKKIDGLTTNVVSLQELLGDKRSRGAFGEVQLEA
                     LVRNVLPVNSFAMQHTFENGTRADCALFLPEPTGTVAVDSKFPLENYHRMFDSQLTEA
                     EQLVAEKQFKLDVKKHVDDIANKYIISNVTSDGAVMFIPAEAVFAELHAYHNDVIEYA
                     MNKRVWVVSPTTLMAVLNTARAVLKDVETRKQVHVIKDELGKLSKDFGRFDQRMKKLA
                     DNIRQAHENAQEVHISSQKISNRFAQIERVELTNKPLDVLDIIDDKED"
     gene            10307..10564
                     /gene="moaD"
                     /locus_tag="PKL42_02975"
     CDS             10307..10564
                     /gene="moaD"
                     /locus_tag="PKL42_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HNU66065.1"
                     /translation="MKIKIFYFARLKETLKFSTEDLDLPDETLTILKLKALLAQRSDT
                     WQEMLMGKLKVRGAINHELVDDLATIHDGDEVAFFPPVTGG"
     gene            10576..11028
                     /gene="moaE"
                     /locus_tag="PKL42_02980"
     CDS             10576..11028
                     /gene="moaE"
                     /locus_tag="PKL42_02980"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HNU66066.1"
                     /translation="MTVRVQTQDFDVGFEIKQLRVARPDVGAVVSFVGQVRDLNGGNT
                     IARLTLEHYPGMTEKSLEAIVNQAKSRWNIMDATIIHRVGTLQPLDQIVLVAVSSAHR
                     NEAFSACEFMMDYLKTEAPFWKKEATAEGEQWLEAKETDETARKRWEK"
     gene            11096..13222
                     /locus_tag="PKL42_02985"
     CDS             11096..13222
                     /locus_tag="PKL42_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNU66067.1"
                     /translation="MSQFLLPQQLTLNISAQQFKFADTSALIATERKSAQHQAWVAQS
                     EAKKAAEFGLNIQQAGFNLLALGEPSTGRTTLMLSAMQEVATQLASPTDLIALYQFDL
                     NGKPLFVKLPAGVGTQLKQAQDNFIRQLAKALPTLLEAKIKENSLKPIQIFLETQINV
                     IVGKIALIQEHASLINYFKLLKQDVLEYLDAWQPSLGSEGEANIDALLSESFFGRYRV
                     NVLVEHAVDALNSAINAPAIYDNDPSLQSLFGGIESAGEAGNAPDFMRLRAGNLLRAD
                     GGTLLLHLRDILADEQNGAQLLEKLHRFLRNGTLQIEDLSSSNSQGGGFVSTQASIPV
                     SVKLVLIATRKDYYALVDEKSDFFNYFPIKIEFSEYVKANAESYHVYAAFIAQKCQQY
                     HCKHFTAEAVAALLQAMHRLIEDQTRLSTKFADLEKLILESAAVADLRGAKLVALEDV
                     KAAISRKYARHSYIEARIRDSIVDNELMINVQGEVIGQINGLTHVDLADASFGSPVRI
                     SANCYAGRQDVLTIDREVAMSGPTHDKGVMILQSWLHANFADSNPLNLTASLVFEQEY
                     SGVDGDSASCAELFAVLSALAQVPIKQGIAVTGAMNQHGEVLPVGGLNEKIEGYFRVC
                     QEIGLDGQQGVLIPKRNSRHLILSDEVVNAVANGKFHIAVMDDIAEGISHLTGQTLGQ
                     INLLATKKLVSFKATLEANSPKILTR"
     gene            13246..13653
                     /locus_tag="PKL42_02990"
     CDS             13246..13653
                     /locus_tag="PKL42_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNU66068.1"
                     /translation="MKEQDEKQDAKCRLDKWLWAARFFKTRSLATAAIGTGKVHVDGD
                     RVKPAKELHVGQLVHIRNKDFEVEVTVQALSNIRRGAPEAQLLYTETTASVTKRENLK
                     VTGENAHALRDRGAGRPTKRQLRDIKRFTGDAY"
     gene            13733..14065
                     /locus_tag="PKL42_02995"
     CDS             13733..14065
                     /locus_tag="PKL42_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family EPS-associated transcriptional
                     regulator"
                     /protein_id="HNU66069.1"
                     /translation="MLTDEYRYKILKLLETNPEISQRELAKILGISLGKANYCLNALI
                     EVGLIKVGNFRTSKNKLAYMYLLTPSGIEEKAKITLRFLTFKLNEYELLKKEISTLKR
                     EVGKESFT"
     gene            14140..15435
                     /locus_tag="PKL42_03000"
     CDS             14140..15435
                     /locus_tag="PKL42_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNU66070.1"
                     /translation="MNVTDKKIAIIGLGYVGLPLAVEFGKTRSVIGFDINTKRIAELE
                     DGQDHTLEVTPEELVNAKFLAYTANSDVLKSASIFIVTVPTPIDQANRPDLTPLIKAS
                     ETVGKALKKGDVVIYESTVYPGCTEEICVPALEKNSGLKFNVDFYCGYSPERINPGDK
                     VNTLTKIKKITSGSTPEIADEVDALYASIITAGTHKASNIKVAEAAKIIENTQRDLNI
                     ALVNELSVLFERLGIDTIEVLEAAGTKWNFLPFRPGMVGGHCIGVDPYYLTHKAEEVG
                     YHPQIIASGRRMNDNMARYAARSVIKLMLKNGIDVSKSKVGVMGITFKEDCPDIRNSK
                     VVDLIKEFQAWGVNVVVADPYADADEVFDEYGVRLNSMDDLSSVDALVVAVGHKVYRN
                     MSPLELKQMLKSEKPVLADLKALYNRHEAAALGLTVFRF"
     gene            15491..16552
                     /locus_tag="PKL42_03005"
     CDS             15491..16552
                     /locus_tag="PKL42_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNU66071.1"
                     /translation="MYHFPPPILNRKIRFALVGCGRIAQNHFAAIKQHSDHAELVGVC
                     DINPVALADAVKTTGAQPFNSLTEMLANCDADVFVLTTPSGLHPDQAIEIAKAGRNVI
                     TEKPMATRLADGKRMVNACDEAGVRLFVVKQNRRNATLQLLKKAIEQKRFGKIYMVNL
                     NVFWTRPQSYYDQGAWRGTWEYDGGAFMNQASHYVDLIEWLIGPLESLQAYTATLERD
                     IEAEDTGVISLRWRSGALGSMNVTMLTYPKNLEGSITILGEKGTVRIGGMAVNEVQHW
                     EFADTDPDDAKVKEASYETTSVYGIGHPLYYDNVIKVMRGESEPETDGREGLKSLEVI
                     IATYLSARDGKRVGLPLAY"
BASE COUNT         4632 a         3383 c         3979 g         4661 t
ORIGIN      
        1 agccgaaact gcccaaaata atgatgctca accaaatacc gacttggatg actatgatgt
       61 cttagtggcc gcagactttt taagttgcta tgtcactttt aaggcaattc aggcggcaga
      121 gcggtctcca agtgctgagc gcaacgaaaa ctttgacata ttgagtgtct accaagctta
      181 tgcgttactg gcctatgctt tttttacgcg ccccttgggt gcagaggaca ttacccccaa
      241 ctttcaaacc gcgcaaatta ctattgcaaa aacattgttt gcggggttgc cagagccaga
      301 attaattgaa attattgaat ctggcttaca taaatttcaa ctcatcgcag acgctgaggt
      361 tgaacactgg actgagttta gagaaaatct cgataagctg acagttgctt ttattgttgc
      421 tggcaccgat gatgaaagcc cgcatggtaa agaagaatta tttccgctat ttggccaact
      481 attaagccaa ctgtgtgagg cgtttgaaaa cgtttaaaac gccttaattt tctacgagaa
      541 aaaaataatg ctctgcgagc ctttatttat aaggctcgca gagcatcaaa aaaggttgtt
      601 tgatccttag ctaaaatatt taagtttttt aggtaaattt caccttgttt caacaatcac
      661 atttgatgag ctgataatct gctcagtctc gttttttgtg caagcgttgg tgcgatgaat
      721 tttgtttcaa tgttagaata agcgcatcca aataacggtt gaaagcctgt gatgtccaca
      781 ccaattctta tcgcaaaaaa tcaagaaact tctaattttc tgttgccaaa aatggctaat
      841 cggcatggtt tgattgcagg ggcaacaggt acaggtaaaa cggtgacctt gcaatcgctg
      901 gcagagagtt tttcagcgca gggtgtgcca gtgtttatgg ccgatgtgaa gggcgattta
      961 gcaggtatga gccaaatagg tggcggcaac gctaaggtgg aagcgcgtgt acagcaattg
     1021 gcgttgactg attttagcta taaaaagagc ccagtggcgt tctgggatgt gtttggcgta
     1081 aaaggtcacc ctgtgcgcac taccattgcg gaaatggggc cgctattatt ggcacgtttg
     1141 ctcaatttaa atgaagtgca gggcggcgtg ttgaattctg tatttaaaat tgccgatgac
     1201 aatggctggc tgttgctgga tttaaaagat ttacgcgcca tgatgcaaca cgcggcagaa
     1261 aattcagcca cttaccaagc gcagtatggc aatatttcgg ccgccagcgt gggcgcggtg
     1321 caacgcagtt tgcttgaatt agaaacccaa ggcgccgacc aattgtttgg cgaaccagcg
     1381 ctgaatctgg atgacttaat gcaaaccgat gcagatggac gcggcatcgt gaatattctg
     1441 gcgtccgata agttgtttaa ttcgccacgt atttacgcta ctttactgct gtggttgttg
     1501 tcagaattgt ttgaaaaatt acccgaagtg ggtgatttag aaaaaccgaa actggtgttt
     1561 ttctttgatg aggcacactt attgtttaac gatgcaccgc aagcattatt gaccaaaatt
     1621 gagcaagtgg tgcgtttgat tcgttcaaaa ggtgtgggcg tgtattttgt cacgcagaac
     1681 ccgttagaca ttccagatgc cgtccttggc cagcttggta accgcgtgca acatgccctg
     1741 cgcgccttta ccccgcgcga ccaaaaagcg gtgaatgccg cggcagaaac ctttagaagc
     1801 aatccaaaag tgaatgtggc cacggcaatt acagaattag gcgtgggtga agcgctggtg
     1861 tcattgttag atgaaaaagg cataccaaca ccagttgagc gcacgtttgt ttgcccgcca
     1921 tccagccgca ttgggccgat taccgatggt gagcgcttgg atgtgatgaa gacttcattg
     1981 gtggctggcg cttatgaaaa aacggttgac cgtgagtcgg cctacgagat tttaaaaaca
     2041 cgtgctacgc agagcgcaac ggaagccaaa gcagatgaag gctttagctg gggcgacatt
     2101 ttgggtggta aaaaaactag ccgttctgac accattttag aaagcgctgc aaagtctgct
     2161 gcgcgcgcgg tgggttcgca acttgggcgc agtattattc gcggtgtgtt gggtagcata
     2221 ttgggcaagg gaaggtagtt tttaagatac aaactcaggt gcaattttga tttctatttt
     2281 agaagcgggg tttgccgaac aaaccattaa caaatcccgc tttattgctg cagcgttgcc
     2341 gtgtgcggat gagcgtgaaa ttggcgcggc actccgtgcg tttgctgcac aacacccaac
     2401 ggcgcatcac ttggcttatg gctttcgcat taaaaccact gatggcatta ttccgcgctt
     2461 ttcagatgcg ggcgagccat ctggcacggc tggcatgccg atactcaagc atattgaagg
     2521 gcgcgatttt atcaatgtgt gtgtagcggt cattcgctac tacggcggta ttaatttagg
     2581 gacgggcggt ttggtgcgcg catacggtgg cacggcaaag cttgctttgg acgcggcaaa
     2641 aacggccgag tatgtagaaa tgcgcagtta cccgttaacc atcagctaca aacaaatgga
     2701 catgctcaca aatgtgctgc caaaatgtaa cggcacaatt ttgagcaaat catttaacgc
     2761 gcaggtggat ttggtgctgt cgctacctgt gaatgaggca gaaaacttcc tgaatcgctt
     2821 taagccttac ggcagttaaa gtttgtagca tgcttttaca ctgggtgtgg tattgtttta
     2881 tcgcgtgatg ccttaatcac ttaatgacca ataacgcatt taaattgaaa ctattcgcat
     2941 gatcaacact tcaaaaaaat taccaccaaa tgtttatgaa attgctgctt gggtagtggc
     3001 ggcgattgtt ttggtttttg tgctggtgtt tcatttgctg cctgcattgt tggcgggatt
     3061 gttggtgtat gaactcgtgc atatgctcac accgcaaatt gccaagcgct ttcctacaaa
     3121 aaaaccaagt aatcgcgcca aactttgggc ggtgggtatt ttggtggtga taattgttgg
     3181 attgctgagt ttagcagggg ccgccttggt ggcgttttta aggtctgatg caggaagtgt
     3241 gacagtttta cttgcgaaaa tggcgcaaat tctggaagat tcacgcaaaa ttttacccac
     3301 ttggttgctg gatcatctgc cagccgatgc ccatactttt cgggagcaag cctccgagtg
     3361 gttgcggtca catgcagatg agctgcaagt ggtgggtaaa gaagcagggc gggcaaccgc
     3421 acatgtgcta attgggatga ttattggcgg catggttgca gtgcgggacg cagtgagaat
     3481 ggatagccgc aaaccttttg cccgcgcgct ggcgacaagg ggcgatatgt ttggcgacgc
     3541 gtttcgtcga atcgtttttg cgcaagtgcg tatttcggcc attaacacca catttacggc
     3601 aatttattta gcggttgtgt tgcctttaat gggcattcat ttaccgttga ttaaaaccat
     3661 gattgccatc acctttttgg tgggcttgtt gccagtcatt ggtaatttaa tttcaaatac
     3721 ggtgattgtg gtggtgagtt taagccagtc attgggcatt gcgattgcgt cattggccta
     3781 tctgattatc atccacaagt ttgaatattt tttgaatgca cgtattgtag gcgggcaaat
     3841 tcgcgccaac gcatgggagc tattaattgc catgttactc atggaagcgg cttttggctt
     3901 agccggtgtg gtggcagcgc caatttatta cgcctacatt aagtctgagt tgcgtcgtag
     3961 agaacttgtg taatagagcg caaaggtaaa tgatggtttc agcgtacttg ttgaatagtt
     4021 ttttggtagc ggcagcggtg ttaattcact ttgaagcact gaatttgctc acacgactta
     4081 ttccgcattt gccgattagg caacgattgc gcattttgtt tggggtattt ggcgcactga
     4141 ttgcccacgt cgttgaaatt tgggtattcg cttttggcta ttattttatg attcaccaag
     4201 gtgggtttgg cacgcttgaa ggccattttg accaaagcct aatggactgc agttactttt
     4261 cgtttgtcag ctacacttca cttggttttg gtgacattgt tgccaaaggc gatattcgct
     4321 ttttggccgg gcttgaagcg ctcactggct tggtgctgat tacctggacc gcctctttta
     4381 tgtttattga aatgcagaaa ttttggaaag accattaaaa aagccgcact tgttaaggtg
     4441 cggcattttt atttcagtta aaagattttg tcttactttt ggttttgtct atcttcaatg
     4501 attttcgctg cataatcttt accccccagc ccaaaagcaa tcgccagtgc caaaaagata
     4561 gcgccaaaca taatgataaa cagcgctgtc acgagttgag agccaatgtc taactgctca
     4621 agcgccacaa aaatcgcaaa tatctgcacc agatattcag ctgaagtgct tactttaagc
     4681 gcaccatcaa atttaatgct gtgcagccag gcaaacacca agcggttcac aaagcggccc
     4741 aataacgtac caaaaatcaa caccacaatt gccacaatga tttttggcag gtagttggct
     4801 aattgtttga gcatgtcagc cacttcggtt aaacccagcg catttgcacc cgtaataata
     4861 aacagcaaaa tcactaacca aaatacaatt tgactaatga tgccgctgag cgacacgtcg
     4921 taatcatcat tttgtaggta ggattcaagc ccggatttac ctgaaaattt gtcaaattta
     4981 acccatttta aaaagcgctt aaccgcgatg cgcgagcctt tagcaatcag ccagccaaaa
     5041 aataaaatga tgatgaccgc gagtaatttt gggaaaaatg aggcgacctg atgccagaat
     5101 tgattgaggg atgaaacaaa aatatcaatt tgatcttgca tgtaaagctc ctttatttgc
     5161 gtgactgcta ttatttttta tgatgacgat atttgcaaaa atgcaaaagt gtctagctag
     5221 cctgatgttg taacgctgaa gatgttgctg tttgaaaaga agttttattg tcgcttaatt
     5281 catgctggct cgcaatgaaa aatcgttttt aaattgctaa ttttcacaga gaaaaaaacg
     5341 ctgccctgcg agccttgtaa ataaaggctc gcagggcagg tcaaaagggt tgtattgaaa
     5401 ttgcgtgtaa aaatgttgtt gatagaagcc tgaataaatc ggtaacgttt aatttttgag
     5461 tgtgtaaaaa cggttttcta aggttttacc attttgcact tggtcaaatt tattgcctgc
     5521 gctactgccc agtttttcca gccgttcatc agggtgcgga tgcgttttaa acagcaacgc
     5581 aacgctatta tcggttttat tggtttggcc tatggtttgc aatacttcgc ctaaactaaa
     5641 agcatcatag cccgcgcggg tggcaagcac catgcccatg cggtctgctt caaattctgc
     5701 gtctttgtcc aaactgcgtg cgctaatttc agcgccgcta tcaatcactt ttttagcaac
     5761 tttatcgtct tttgcaaaca tttcacccag ttttcctgcg ccataaccta ataattgctg
     5821 tttttgcagc actttcagct gatgcttttt aaccacatgg gcaatttcat ggcctagcac
     5881 gctggctaat tgcgcttcat tttgcagctt ttggtacaag cctttggtca ccagcacata
     5941 accgccgggc gcggcaaaag cgtttaaatc attgctttca atcacgccga atttccacgg
     6001 caggtcaggg cgttcagatt gacttgccac ccagcggcca acgttgttca cgtatttttg
     6061 caatgcagca tcattcacca aaggggcggc ccctaataca ctgccagaaa tttcacgccc
     6121 aagcgcgact tcttctgcgc ggcttgggtt tttccagtta aatggtgtgc tttcagcggg
     6181 tgttgatgtg gcatcagcgt ttgcagtcgc ttgaatattg cttgcgttgc tggcggcagc
     6241 ctctgttttt tgaggttctg aatttgtcga attgtccgtt ttaatctctt caatcttttg
     6301 cttggcagcc ttttcaagcg cttttcctaa atccaacgct tgggtttgtg ctgaagctaa
     6361 cgcagtaacc aagcccgcaa caacaaaaaa taattgacga tattgaaatg tggcggctga
     6421 cattattttg ctccttttaa ataagcaaac tgaacgcttt ttaagccgcc tgctttggca
     6481 aatttttggc tatcttcggc gctcactgca tagctttcta gttttttcat ttcttcttca
     6541 ttaaattttg cggctttgag ttcttctgca cttaaaccgc gaatgccagt ggtagaaatc
     6601 actttgccag tacttgcgcg gccgctggca acatcgtaaa cgcctttggc ctgattggtg
     6661 ttactggcgc tgccgcgttt gacggagaat attctgaccc agccagtatt tttttgcgtt
     6721 ttaatgtgta gccacgcgcc ttttttgtta agaatttcta ccgtttcatt tttggcgatg
     6781 gtgcctttcg tttttgcgtc tgaaaatggt tctacgcgca aggtatccgc ttttagcgcc
     6841 gtgcctagtt ctgcggcaat acttatggcg ggcagcagta gcgctaacag cagccatttt
     6901 tttgagtgaa gcttcgtgaa ttgtttcatg ctaaatcctt taaagatgcg tggtcgtttt
     6961 gctctagttt aattaccgct tgcgcaaaca gcgcccgcca ttgtgaggca agggcggcgg
     7021 cactgtcatt ttcaagttgc ccttcatgca agctataacg aataatcgta ctggttggta
     7081 gatccaacgc ttggcttaaa tgaggtaggg attttgctaa aagttgcgca tcgtgcttgg
     7141 ccgctgtaac gtattcgtct ttttggtcag aaaactgcca agtgaccacc accatatcgc
     7201 caaacagccc ccaaataccg ccttgtgtgc cgtgtagcac ttcaatatta ctcggtgttt
     7261 tgctggtgag ttcgatttgg cgcttaaatt ttcgtaaaag tgcgtcattg agtgcatcat
     7321 ggtttaaggg gtcgttactt tcaagcaaaa tgggcatgat caatgctaac gttttgctgt
     7381 cacccgattt aacgccaaaa gtcagccagc gttgtagcgc gcggtcattc gccagtgcgt
     7441 agatttttgc aatcccaaaa aacgccactg cccataaaat aggcgcggtg aggtcgaggt
     7501 aagtattggt gaaattaatg gcgaggtacg acaacaccag tagccccatt tggccgctgg
     7561 tgaagatggt gttaaatcgg tcgcggtcca tactgcggta aaacgccaag gtggtgagcc
     7621 aaattaatag caaactcaat aaaatatatg gcgttttgcc gcgccacact tttaaataat
     7681 ctcgattttt aacgttatca atggcagtgg ccaaaatttc aacgcccgga aattgcttgt
     7741 ccatggctgt ggctttaatg tcaaacagcc caggcgcggt tgaaccgata atcacaattt
     7801 tattggtaaa ttcattttgc gggcgctttt taaccttgct ggtgaggtca gtaaatacat
     7861 cgctaaacgg cacgtattga tagctaaagg gcttgccgcg ccagttgatg agcatgtttt
     7921 gcggtagggg gtatgaattc atctgcgaaa aatcgccaac aattagcggc aaagacggca
     7981 aaaaccagcc gtaatcatca tggtagatgc gatattcacg cacaatgcca tcaatatcag
     8041 ggtaaacgtt gtgcgtgccg agtcgattgg ccttaatggc gccctcaaaa tgcggcaaaa
     8101 tcgcggcaaa cgtggcgtct tgatttcctg agtcctgttt aaattttaag ccgggaattt
     8161 gtgaaaattt aagctcacta tggcggtctt ggctaatatc caggcgcaaa ataggaaaaa
     8221 acgtgttatc agcgctagaa atcacctcat taaaatattc atcgccttct ggcctaaaaa
     8281 tatcggcatc gctaaacagc acgtcaaata caatcgcttt gggcttttgc gcctgaatgt
     8341 tttcaacaaa ttcgcccatc acttggcgcg accaaggcca actgccgaat tctttagcaa
     8401 acgcgtgcaa actggcttcg ttaatgtcca caatgataat gtcagggtca gcttgtggtt
     8461 gaattacacg gtacttcacc ataaaatcaa acgcacggtc acgcatgttt aaacccacac
     8521 caaacacgct ggcatccagc aacaccagca cggtgaacag ggcagctaaa taaatgtaga
     8581 aattgttttt aagcttgcgc gtcatattgg caagccattg gcctaacaaa agtttcaatt
     8641 tagtcatttg cgcaatcagc gagtggttaa gcctaaaatc ataaatcttt ttggcggctt
     8701 tgtgggtttg atttggcggt ttgtgaaatt taaattgcct aaaattagcg ccgcaaaaat
     8761 acactttatg gatgtgattt ttaacccaac ctttttcgat gctctggaag ccttataaat
     8821 aaaggctcgc agaggagaaa atttttctcg ggaggaaaat gactgaaatt caccgtattt
     8881 tcgacgtgaa taacacgctt tagtttgcta gcaatttgca cgattgcgca aaactattta
     8941 gcgcgcatcc gttataatac ggctcaatga atatgatttt tttacaatgg attgcaacgc
     9001 tcattacctt gcttttgtta gcatggttgc tgtggcgtag ttaccaaaac aaatccaccg
     9061 aattgctatt gcaacagctt gaagacaagc accgcgctat gctgcttgat ttaaatgatg
     9121 gcttgaataa attgggcgac cgcatcaatg cagcctcgca agagggcagt gagcgcttaa
     9181 agtcagccgt ggccacagag ttgcaagcca cgcgcgaggc catgcaggct ttgcagttag
     9241 cgcaaaataa cagcttggcg caaacccgcg aaaccgtgct ggaaaaactg cacaccacct
     9301 tgagcgagca aggtaaatct caacaggcgc tgattaacga taccatgctt aaagccacca
     9361 ccacgctcac acaaagtgtt gaaagcttgg gaaaaattgt ggatagtcgt ttggagcaaa
     9421 ttggcggaaa agtgtctgag cgcttggatg aaggctttaa aaagaccaat caaaccttta
     9481 ttgatgtgat ggcacgattg gccaccattg atgaagcgca aaagaaaatt gatggcctca
     9541 ccaccaatgt ggtgagtttg caggaactct tgggcgataa acgcagccgt ggcgcgtttg
     9601 gcgaagtgca gttagaggcc ttggtgcgta acgtgttgcc agtcaattca tttgccatgc
     9661 agcatacctt tgaaaatggc acacgtgccg attgtgcctt atttttacct gaacccacgg
     9721 gcacggtggc ggtggatagc aagtttccgc tcgaaaatta tcatcgcatg tttgacagtc
     9781 aattaactga agccgagcaa ttggtggctg aaaaacagtt caaacttgat gtcaaaaaac
     9841 atgtggatga cattgccaat aaatacatta tttctaatgt cacgtcggac ggcgcggtca
     9901 tgtttattcc tgctgaagcc gtatttgccg aactgcacgc ttatcacaat gacgtgattg
     9961 agtatgccat gaacaagcgc gtgtgggtgg tttcgcctac cacgctcatg gccgtgctca
    10021 atactgcccg tgctgtgtta aaggacgtgg aaacgcgcaa acaagtccat gtcattaaag
    10081 atgagctagg caaactcagc aaagattttg gccgctttga ccaacgcatg aaaaaacttg
    10141 ccgataatat tcgccaagca cacgaaaacg cgcaagaagt tcatatcagc agccagaaaa
    10201 tttcaaaccg ttttgcgcaa attgaacggg tagaactcac caacaagccg ctggatgtgc
    10261 tcgatattat tgatgataaa gaagattagg tttacatcat aacaccatga aaattaaaat
    10321 attttatttt gcaagactta aagaaacgtt gaagttttca actgaagact tagatctgcc
    10381 agacgagacg ctgacgatac taaaactcaa agctttgtta gcgcaacgca gtgatacttg
    10441 gcaggaaatg ctcatgggca agctcaaagt gcgcggcgcg attaaccatg aattggtgga
    10501 tgaccttgca accattcatg atggtgatga ggtggcattt tttcccccgg taacaggtgg
    10561 ttagttagtt ggcacatgac agtacgcgta caaacacaag attttgatgt aggttttgaa
    10621 atcaagcagt tacgtgttgc ccggcccgat gttggggctg ttgtgagttt tgttggtcaa
    10681 gtaagggatt taaatggcgg taatacgatt gctaggctca ctttagagca ttatccgggc
    10741 atgactgaaa agtccttaga ggccatcgtg aaccaagcaa aaagccgttg gaatatcatg
    10801 gatgccacta ttattcatcg tgttggcacg ttacagccgc tggaccaaat tgtgctggtt
    10861 gccgtcagca gtgcgcatcg taatgaggcg tttagcgctt gtgaatttat gatggattac
    10921 ttaaaaacag aagcaccatt ttggaaaaaa gaagcaacgg cggaaggcga gcaatggctg
    10981 gaagccaaag agacagatga aactgcccgt aagcgctggg agaaatagcg ttggtttact
    11041 caacattcat tcacaatagc aattcaatag atacaccata aaataacaag gcaatatgtc
    11101 acaattttta ttaccgcaac aattaacact gaatatttca gcgcagcaat ttaagtttgc
    11161 tgatacgtcc gcgcttatcg caactgagcg taaaagtgcg cagcaccaag catgggtggc
    11221 gcaatctgaa gcaaaaaaag cagcagagtt tggcttaaat attcagcagg cagggtttaa
    11281 cttgctagca cttggtgagc ctagcaccgg gcgcacaacg ctgatgttaa gtgccatgca
    11341 agaggttgcc acacagcttg caagccccac tgatttaatt gcactttatc aatttgattt
    11401 aaatggcaag cctttatttg ttaagcttcc agcaggtgta ggaacacaat taaagcaagc
    11461 gcaagataat tttattcggc aattagccaa agcgttacca accttacttg aggccaaaat
    11521 aaaagaaaac tcactcaagc cgatacagat atttttagaa acacagatca atgtaattgt
    11581 cggtaaaatt gcgttaattc aagagcatgc ctccttgatt aattatttta agttgcttaa
    11641 acaagatgtt ctggaatatt tagatgcttg gcagcctagc cttggcagtg agggtgaggc
    11701 caatattgac gcgttgttga gcgagagttt ttttggtcgt taccgcgtga atgtgttggt
    11761 tgagcatgcg gtagatgcgt taaattcagc gattaatgcg ccagcaattt atgacaatga
    11821 cccaagcttg cagtcattat ttggtggtat tgaaagcgca ggcgaagcag gcaatgcgcc
    11881 agattttatg cgtttgcgtg ccggcaacct tttgcgtgca gacggcggca cgctgttgct
    11941 ccacttgcgt gatattttgg cggatgagca aaatggtgcg cagctccttg aaaaattaca
    12001 tcgtttttta cgtaacggca cgttgcaaat tgaagattta agcagcagta atagtcaggg
    12061 tggtggtttt gtcagcacac aggcgtccat tcccgtttca gtcaagctgg tactaattgc
    12121 aacgcgtaaa gactactacg cgttagtcga tgaaaagtct gatttcttta actactttcc
    12181 tattaaaatt gaattttccg aatacgtaaa agccaatgca gaaagctatc atgtgtacgc
    12241 ggcttttatt gcacagaaat gtcagcaata tcactgcaag catttcaccg cagaagctgt
    12301 ggcagcgtta ttgcaagcga tgcaccgctt aatagaagac caaacacgct taagtacaaa
    12361 atttgccgat ttagaaaaac tgatcttgga aagcgcggct gtggctgact tacgcggtgc
    12421 taaacttgtc gcgcttgaag atgtaaaagc agcgattagt cgtaaatatg cacggcatag
    12481 ttatattgaa gcgcgcattc gcgattctat tgtagataat gaattaatga ttaatgtgca
    12541 gggcgaggtg attggccaaa ttaacggcct gacgcatgtt gaccttgcgg acgccagctt
    12601 tggctcgcca gtgcgaatat ctgcaaattg ctacgctggc cgccaagacg tgctgacaat
    12661 agaccgtgaa gtggccatga gcggccccac gcacgataaa ggcgtgatga tattgcaaag
    12721 ctggctacat gctaattttg cagactcaaa tccattaaac ctgacggcgt cattggtgtt
    12781 tgagcaagaa tacagcggcg tggatggtga ctctgcatcg tgcgcagagt tgtttgcagt
    12841 attgtcagct ttggcccaag tgccaattaa gcaaggtatc gccgtgacgg gtgcaatgaa
    12901 ccagcatggt gaagtgttac ctgttggcgg cttaaatgaa aaaattgaag gctattttag
    12961 agtttgccag gaaattggtt tagatggtca acaaggcgtg cttattccca aacgcaattc
    13021 acgacattta atactgagtg atgaagtagt caatgcggtg gcaaatggca aatttcatat
    13081 tgccgtcatg gatgatattg ccgagggaat ttcgcatctc actggccaaa ccttagggca
    13141 aatcaatcta ctggcaacta aaaaattggt ttcatttaag gctactttag aagctaattc
    13201 gccaaaaata ttaactagat agaagataaa tctctaagaa ttaacatgaa agagcaggac
    13261 gaaaaacaag atgcaaagtg caggctagat aaatggcttt gggctgctcg tttttttaaa
    13321 acgcgaagct tagccactgc tgcaatagga acaggcaaag tgcatgttga tggcgaccgt
    13381 gttaaacccg ccaaagaact gcatgttggc caacttgttc atattcgtaa taaagatttt
    13441 gaagtggaag ttacagtaca agcgctatca aatatacgta gaggcgcccc agaagcccag
    13501 cttttatata ctgaaaccac tgcaagtgtt acaaagagag aaaacttaaa agtaacaggt
    13561 gaaaatgcac atgccctgcg agataggggt gcaggcaggc caaccaaacg acaactgcga
    13621 gatattaaga gatttacagg cgacgcttac tgatttttta caattttctt tgaacttatt
    13681 aatctttaat ataaactgat aatccattcg ttcaatagtt gaactcctag atatgcttac
    13741 agacgaatat cggtataaaa tacttaagtt gttagaaaca aatccagaaa ttagccaacg
    13801 tgagttggct aaaattttgg gaattagtct tggaaaagct aattattgct taaacgcttt
    13861 aatagaagtt ggcctgataa aggttggtaa ttttcgaaca agtaaaaaca agttagcgta
    13921 catgtattta ctcacgccaa gtggcataga ggaaaaagct aaaattacac taaggttttt
    13981 aacttttaaa ttaaatgaat atgagttgct caaaaaagaa atttctactt taaagcgaga
    14041 ggtaggaaag gaaagtttca catgaatttg gttgaaaatc accttttgct ggggtaaagc
    14101 tttttaataa ttacactaat cttaacgaga taaatataaa tgaatgtaac tgataaaaaa
    14161 atcgctatca tcggactagg ctatgtcggt ttgccattgg cggtggagtt tggtaaaacc
    14221 agatctgtaa tcggcttcga tatcaacaca aaacgcattg cagagctcga agacggccaa
    14281 gaccacactt tggaagtaac gcctgaagag ttggtaaatg caaaatttct agcttacaca
    14341 gcaaactctg atgtgcttaa atcggccagc atttttattg ttaccgttcc taccccaatc
    14401 gatcaagcga atcgccctga cttaacacct ttaattaaag caagcgaaac agtcggtaag
    14461 gcgctaaaaa aaggggatgt cgttatttat gaatctacag tttatcctgg gtgtactgag
    14521 gagatttgtg ttcctgcttt agaaaaaaac tcaggtttaa aatttaatgt cgatttttac
    14581 tgtggatata gtcccgagcg aatcaatcca ggtgataagg ttaataccct tacgaaaata
    14641 aaaaaaataa cgagtggctc aacgccagag attgcggatg aagttgatgc gttatatgcc
    14701 tcaattatta ccgcaggcac gcataaagct tcgaatatca aagtggcaga agccgccaag
    14761 attattgaaa acacccagcg cgatttaaat attgcattag tcaatgaatt gtcagtgttg
    14821 tttgagcgtt tgggtatcga cactatagag gtgctggagg ccgcaggaac aaaatggaac
    14881 ttcttgccat ttaggcctgg tatggtgggt ggtcattgta ttggtgttga cccatattat
    14941 ctaacgcaca aagctgaaga ggttggctac cacccgcaga tcattgcttc tggtcgccgc
    15001 atgaatgaca acatggcaag atacgcagcg cgaagtgtaa taaaattaat gttgaaaaac
    15061 ggtattgatg tttctaaaag taaagtcggt gtgatgggca ttactttcaa agaagattgt
    15121 ccagatatac gcaacagcaa agttgtagat ctgattaaag agtttcaggc ttggggtgtc
    15181 aatgtggtgg ttgcagaccc ttacgcagat gcggatgaag tgtttgatga atatggcgta
    15241 aggcttaatt caatggatga cttatcttca gtagatgctt tagtggttgc tgtgggtcat
    15301 aaggtttatc gcaatatgtc gccattagaa cttaaacaaa tgttgaaatc tgaaaagcca
    15361 gtgctggctg atttaaaagc actttataac cggcatgaag ctgccgcatt aggtttaact
    15421 gtgtttagat tctagggccg atatttctaa tctaaatttt aagaatatac aaataaaaga
    15481 gacgctcgat atgtatcatt ttccgccacc aattttgaac agaaaaatcc gattcgcact
    15541 cgtaggttgt ggacgcattg ctcaaaatca cttcgctgct attaagcaac atagtgacca
    15601 tgctgaactg gtgggggttt gtgacatcaa tccagtcgca ttggctgacg cagttaagac
    15661 gacaggtgca caaccattta actctctcac tgaaatgctt gctaattgtg atgccgatgt
    15721 gtttgtttta acaacaccgt ctggtttgca cccagatcaa gccattgaga ttgccaaagc
    15781 aggccgtaat gtgattacag agaagccgat ggcaacgcgc ttggcagatg gtaaacgcat
    15841 ggtaaacgca tgtgatgaag ctggagtgcg cttgtttgtg gtgaagcaaa atcgtcgtaa
    15901 tgcaacattg cagttactta aaaaagccat tgagcaaaag cgcttcggta aaatttacat
    15961 ggtgaacctg aacgtgttct ggacacgtcc acaatcatat tatgatcaag gagcatggcg
    16021 cggcacatgg gagtatgatg gcggtgcatt tatgaaccaa gcgagtcatt acgttgattt
    16081 gattgaatgg ttgattggcc cactggaaag cttacaagcc tacaccgcaa ccctagagcg
    16141 tgatattgag gctgaggata ctggtgtcat tagcttgcgt tggcgcagtg gcgcgttggg
    16201 ctcgatgaat gtaaccatgc tgacttaccc taagaactta gaaggaagca ttactatctt
    16261 gggagaaaag ggcacggttc gtattggtgg catggctgtc aatgaagttc agcattggga
    16321 atttgctgat accgaccctg acgacgcaaa agtgaaagag gcaagctatg aaacaacctc
    16381 tgtctatggc attggtcacc ccctctatta cgataacgta attaaagtga tgcgtggcga
    16441 gagtgaacct gaaacagatg gtcgtgaagg cttaaaatct ctggaggtga ttattgcaac
    16501 ctacctttct gcgcgtgatg gtaagcgcgt tggtttgcca ctagcttatt aggtatttga
    16561 tgcatatagc tgttgttggt gggggtatta acggtttaag ttgtgcttgg cttcttgctc
    16621 aggctggtta taaagttgat ttatatgacc gtggt
//
LOCUS       DAOQSI010000025        16480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_2602_length_16480_cov_5.315190, whole genome shotgun sequence.
ACCESSION   DAOQSI010000025 DAOQSI010000000
VERSION     DAOQSI010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 16480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16480
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2602_length_16480_cov_5.315190"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..635
                     /gene="wrbA"
                     /locus_tag="PKL42_03010"
     CDS             42..635
                     /gene="wrbA"
                     /locus_tag="PKL42_03010"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HNU66072.1"
                     /translation="MTEILILYYSQGGAVRDMAQLVARGVESVPNIKARIRTVPKVSA
                     NCEATEADIPSTGDPYVELCDLEECIGLALGSPTRFGNMAAPMKYFLDSTTALWLKGA
                     LIGKPAAVFTSTGSMHGGNESTLLTMMLPLMHHGMVMVGLPYSEPELSSTQTGGTPYG
                     ASHVAGAMDDKPLSADERKLCMALGKRLGEIALKLNA"
     gene            698..1423
                     /locus_tag="PKL42_03015"
     CDS             698..1423
                     /locus_tag="PKL42_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HNU66073.1"
                     /translation="MRLANEALQFLRSTRSGVLSTFSAKYAGYPFGSVAPFVLDHSGQ
                     PVILISTIAEHTHNIVANPKVSLLVFAGDDDLQANARLTLIGEAKLIDKQDVDLRARY
                     LRYLPQAASYFDMHDFNFYRIEIAHIRYIAGFGRMGWIDGTELTHTQLLASSALATQE
                     ASIIEHMNTDHVHSLVAYCKHFHQIEATHAQMLGIDRDGFDVKVTDAQHNTQVLRFHF
                     NQPIEDAQSARVALVAMSKAATT"
     gene            1420..1764
                     /locus_tag="PKL42_03020"
     CDS             1420..1764
                     /locus_tag="PKL42_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HNU66074.1"
                     /translation="MIQILRLGASISLISLILLCLCWEAFLAPLKPNGSMLILKTVPL
                     LLPLFGVLHGKRYTYQWASMFILFYFTEGVVRAWSDTGLSANLALIEVVLCVIFFVCA
                     IFYAKLTRKPVQ"
     gene            1830..2573
                     /gene="surE"
                     /locus_tag="PKL42_03025"
     CDS             1830..2573
                     /gene="surE"
                     /locus_tag="PKL42_03025"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNU66075.1"
                     /translation="MKILLSNDDGYFAPGLNILAEHIAQLAEVMVVAPERNRSGASNS
                     LTLDRPLSVRKASNGFYYVNGTPTDCVHIALTGLMEDMPDMVISGINDGANMGDDTIY
                     SGTVAAAMEGYLLGIPSIAVSMSQHNSTHFETAAKVAVELVQHYQKHGFSSPTLLNVN
                     VPDIPYNELQGTVVTRLGKRHKAEPVVQLKTPRNETVYWVGAAGLPYDGGEGTDFYAV
                     ANHKVSISPIQVDLTNHAQLSDINDWLKS"
     gene            2589..3278
                     /locus_tag="PKL42_03030"
     CDS             2589..3278
                     /locus_tag="PKL42_03030"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNU66076.1"
                     /translation="MRGTPKLALTRPPTQTGIGMTSLRTRERMLTRLRDQGIKDEVVL
                     SAISAIPRHIFVDEALSIRAYEDVSLPIGFGQTISQPYIVARMSEVLRNGKPLQKVLE
                     VGTGCGFQTAVLSKLAKEVYSVERIRPLVMKARGHLRELKCINVRLGHSDGAMGLPEH
                     APFDGIIVTAAASHIPQDLLDQLAVGGRLVIPVGVDEQILVLVERRTEADYRQTKLEP
                     VKFVPLLGGVS"
     gene            3333..4370
                     /locus_tag="PKL42_03035"
     CDS             3333..4370
                     /locus_tag="PKL42_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNU66077.1"
                     /translation="MTLTACGSNPPAPVTDRTPTKPSKPVANSQSTNTSSKANRPTYK
                     PGDWRPDNYTVKKGDTLYSIGLELGYYYKDIAQANNIGAPYLIKVGQVLNLAALKEKV
                     VVTEVKPEAATGTQPDPQDQDGVIITPIGAETASPNATIESTETPAAVAIVAITEPKA
                     IRELYSDEALKKPLPTPKPITVKPSTPTEVKLDTKPVEVKPTEAKPAVAPTLKPETKT
                     TAESPEDMEWSWPTKGKVVGNFSEAGNKGIDIAGSLGQAITAAAPGKVIYSGSDLRGY
                     GNLVIIKHNATYLSVYAHNSAILVKEGQQIARGQKIAEMGNTDSNTVKLHFEIRRQGK
                     SVDPSKYLAAN"
     gene            4392..4757
                     /locus_tag="PKL42_03040"
     CDS             4392..4757
                     /locus_tag="PKL42_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66078.1"
                     /translation="MAKNPREKVDPNKYLKNKDIQAESKLSVLIAVIKMGLVLAGIAG
                     VAMEFFRDGGWLKTALSWLFDSTEHMMFIPVIIFAMWFLNKIFSAAGKGETKKSGDFP
                     MYIMMAIGAYYIFLYLTAA"
     gene            complement(4966..5337)
                     /locus_tag="PKL42_03045"
     CDS             complement(4966..5337)
                     /locus_tag="PKL42_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNU66079.1"
                     /translation="MAVVTLTKENFKDTIVNNPFVIVDFWAPWCEPCVSFTPIFEAAA
                     NNNPDILFGMVDTEAAPEIAEYFEVTKIPGILVIREQAGIHAQQGEIGAPAFDEIIKW
                     ARDFDMTPVRAYYKEEAKKAN"
     gene            complement(5653..6252)
                     /locus_tag="PKL42_03050"
     CDS             complement(5653..6252)
                     /locus_tag="PKL42_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNU66080.1"
                     /translation="MKKPANTQAPIHELIANRWSGRAYDASQPVTQTQIISLLEAARW
                     APSCFGDQPWRFIVWDKNTDASAWQQALDCLVPGNQAWAKDAPVLVLVAADSLFGHNQ
                     QPNRWAQYDTGAAAENLCLQAEALGLMAHQMGGFNPDLARESFNIPSQYTLMAFITVG
                     HPADIATVTGEALTRETAARTRKPLAELFFSESWGKPIV"
     gene            complement(6275..7354)
                     /locus_tag="PKL42_03055"
     CDS             complement(6275..7354)
                     /locus_tag="PKL42_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HNU66081.1"
                     /translation="MANDLFSPVQLGSIALKNRIVMAPLTRNRAGENGVPNDLNVTYY
                     AQRATAGLIITEATPISPLAHGYPALPGIYTDAQVAGWKKITDTVHAKGGKIVIQLWH
                     VGRISHPSLLPNGALPVAPSAIKPAGQAFTYQGLQDYVTPRALEANELPAIVQDYVHA
                     TKAALAAGFDGVEVHAANGYLLDQFLRDGSNKRTDNYGGSVENRTRLLLEVVDAVVKT
                     AAADKVGVRLSPVNPFNDMHDSNPQALFEYVAEALNRFNLAYLHAVEGGIHGGGKADP
                     FDFVAFRKRFKNGYIGNLSYDKARGNAAIASGHADAIAYGVPFIANPDLVERFNKDAP
                     LNVADAKSFYGGTEKGYTDYPFLEV"
     gene            complement(7502..8098)
                     /locus_tag="PKL42_03060"
     CDS             complement(7502..8098)
                     /locus_tag="PKL42_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNU66082.1"
                     /translation="MKHTLIAAVSAVFFSSTAFAAPELYNIDTAHSFANFSIRHVVAK
                     TSGSFNDIQGTIKIDRENLANSSVDARISVASISTGHAKRDDHIKKPDYLDAAAFGQI
                     TFVSTKVEAKSATEGVLTGQFTLHGVTKELSFPFTVLGFGKDPWGGERSGFEARTTIK
                     ASDFGYEWMKKEGAPVGDDIEVTLLIEGVKPAPAKDKK"
     gene            complement(8186..8884)
                     /locus_tag="PKL42_03065"
     CDS             complement(8186..8884)
                     /locus_tag="PKL42_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNU66083.1"
                     /translation="MITIRKSQDRGYAKHDWLESFHSFSFADYYDAAHMGYSVLRVIN
                     QDKIAAGTGFGMHGHRDMEIITYMLAGELKHQDSMGNGSIIRADDVQYMSAGAGVRHS
                     EINPSAEHEAHLLQIWLLPSVLGLPPNYAEKHFLPEQKRNCWCLVVSSDGREGSIQIQ
                     QAVDLYASVLDAGSQLSKTLQDNRRAYLQIAKGEVQCNGVQLSAGDAAKIEAETAIEL
                     IAMAEAEVLWFDLP"
     gene            9002..9919
                     /locus_tag="PKL42_03070"
     CDS             9002..9919
                     /locus_tag="PKL42_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNU66084.1"
                     /translation="MTLKISIEALEVIDAIARKGSFAAAAESLFHVPSALTYTVRKLE
                     EDLGVTLFDRSGHRARLTDAGTELLNEGRHLLDAALALESHVKRVATGVETHIGIAIN
                     DLFNMQPIYNLLQAFYAQGFGTRVKLTREVFGGSWDALLSGRADISIGAPGEAPSGGG
                     YATKLLGHLEFVFAVAPHHPLASLQEPLENQTIIKHRAVAAADSSRNLPPRTSGILSG
                     QDVLTVPDMQSKLEAQMNGLGVGYLPIQLANHYATQGKLIIKQVAEPKTIAPTFLAWR
                     NNRKTGIGKAQQWLLKRLERLTLEELLKN"
     gene            10039..10938
                     /locus_tag="PKL42_03075"
     CDS             10039..10938
                     /locus_tag="PKL42_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNU66085.1"
                     /translation="MKNLTNQILAAVNEVVLGKEAQVKLSLACIIARGHLLIEDQPGM
                     GKTTLAHTLATVLGLKYQRVQFTSDLLPADILGVSVYDRNSASFKFHHGAVFTNVLLA
                     DEINRATPKTQSALLEVMEEAQVTIDGNTYPLTQPFFVIATQNPVQHIGTFPLPESQL
                     DRFLMRISLGYPDHASERALLKNQGGRAIAGQIKAVCDARTLIGLQHVASKIHVSDGL
                     LDYLQALVEYTRSHFATGLSPRSSLGLLQCARAWAMCEGRDFVIPEDIKTVLPSVVGH
                     RLQSSDNTHVAQQILDSIAIPKL"
     gene            10954..11922
                     /locus_tag="PKL42_03080"
     CDS             10954..11922
                     /locus_tag="PKL42_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNU66086.1"
                     /translation="MQAQFWPLTFWKRDWWQQWFRVSESSNNVAVLGSRQIYIVPTRW
                     GLLYAVMVFLLLLGSINYSLSLGFYLTFLLTSLGCVAMFHTWLNFAHLEIEILSAKPV
                     FAGQEALVHIQVKEYKNQQRHSIAAHFDAESAVLHTIPALGAQLFAVPISTRQRGWLS
                     LPKLVFYTEFPLGLFHAWAIVRHPMNIMVYASPSENPSPVVPVVSGLASASPHASQQG
                     DDDFIGHKSYQIGDAPSRLDWKASSRGVGMLTKRYSGNAQEILWLDWATTQGLPMEER
                     IAMLTKSVIDAHSTQLTFGLKLPGNTVQPGQDEAHYHRCLQVLALL"
     gene            11919..13943
                     /locus_tag="PKL42_03085"
     CDS             11919..13943
                     /locus_tag="PKL42_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HNU66087.1"
                     /translation="MNTSPKPQHFKWLLATLALILLLHAGNLPIWVVIISALFGIWRF
                     FILSKNWPMPKLYILAPITIGIGLGIIFTFGGMFGRDTSLALLVSMLSLKLLETKTKR
                     DFYLVVILGYFVVGKLFLFNQSMFTFVLSIAPLALLTAALVQINSTHAQPSTYLLKYA
                     AKMLLQAIPLMLVLFVLFPRIPGPLWSLPQDAHSKIGLPGLSDSIKFNQVSQNAQDNS
                     VAFRVKFKGDVPAQQQLYWRGPVLLTSDQDEWNISNQAGYINQEPLTIGGKPIEYSIT
                     LEPHKQKWLLMLDMPSQVPDFATFTHDYSVVAKEPVYTRIRYDATSYAHYRLGANRLT
                     QREKNLSLQLEEGVNPRTIALAEQWIDLNAEDTVNQALKLFREKDFYYTLNPPILGKD
                     PIDDFLFNTKRGFCEHYAISFVVLMRAAGLPARVVTGYQGGELNPHDNFLIVRQMDAH
                     AWAEVWLKDRGWVRVDPTAAVAPTRIERGIVEAARVARPAPGQTKAETSNLPVAARTK
                     DYPWLHQMMLRLDSIDNGWNQWVIGYNQKKQQAVLSQITGQNVTVNTLLIWLICALML
                     LGGLTLFIIFRKNRQKQSRTQQLYGQYIRALKPFDLAPKPHEGALEFAERVAIALPQL
                     KKEVFNIAESYNTLQYSRFSSLPDTTLLADFEKTIQQFNPKSTQQSHAKH"
     gene            13930..14409
                     /gene="tadA"
                     /locus_tag="PKL42_03090"
     CDS             13930..14409
                     /gene="tadA"
                     /locus_tag="PKL42_03090"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HNU66088.1"
                     /translation="MQNTNQPLTDETGMRAAIELAKLAALRGEVPVGAIVVKEGVIIG
                     RGSNAPIEHHDPSAHAEIIAMREAAALLGNYRLVGCTLYVTLEPCAMCSGAIQHARIA
                     RLVYGAQDPKTGACGSVVDLMAETKLNHHTEVVSGVLAQECGALLSDFFRARRLAKL"
     gene            complement(14491..15183)
                     /locus_tag="PKL42_03095"
     CDS             complement(14491..15183)
                     /locus_tag="PKL42_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HNU66089.1"
                     /translation="MSENIQVLGRETTAVEAQNKVLRNTYAMLGMTMIPTVIGAVVGI
                     NMNFAWMMASSPIMVFIGIFAAVFGMTWLIAKNANNAAGVWLLFAFTFLMGVLLGPIL
                     QYALNLSNGAQLIGLAAGGTAISFLTLAGVASNTKRDFSFMGKFLTIGFVVVIAAILA
                     NFFFQIPALSLTISAVVVMLMAGFILYEINQIVRGGQTNYILATMSIYISLHNMFTNL
                     LHLLMAFMGGDD"
     gene            complement(15543..>16480)
                     /gene="nagZ"
                     /locus_tag="PKL42_03100"
     CDS             complement(15543..>16480)
                     /gene="nagZ"
                     /locus_tag="PKL42_03100"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HNU66090.1"
                     /translation="YENNRQLKALTDSIHAVRQPTLLIAVDHEGGRVQRFREGFTKIP
                     PMREFGKIWDKNPKKAKELATEAGWILASELRAHGVDFSFTPVLDMDYGDSLVIGDRA
                     FHLNPQAINDLAFALMQGLKKGGMAAVGKHFPGHGFVAGDTHVSMPVDERSFDKIAQN
                     DMQPFRMLIDDGIQAIMPAHVIYTSVDDKPAGFSPKWLQKVLRERLGFNGVIFSDDLS
                     MEAASAGGDVTTRALAALNAGCDMVLLCNQPAMADELLANLKWTISAQSLSRLARMHG
                     HRQPPTLDALHENGDFVKAVHQVAQIGITEGELFV"
BASE COUNT         4491 a         3982 c         3741 g         4266 t
ORIGIN      
        1 tgaattgatg caatctgtaa acctaaacaa cctaaaacct catgactgaa atcttaattc
       61 tttattactc tcaaggcggc gccgtgcgcg acatggcgca actcgtagca cgcggcgttg
      121 aaagcgttcc aaacattaaa gcacgcattc gcacggtgcc taaagtatcc gccaattgtg
      181 aggcgactga agctgacatc ccctcaacag gcgaccccta cgttgaattg tgtgatttag
      241 aagaatgcat aggcttagcc cttggtagcc caacgcgttt tggcaatatg gccgcaccaa
      301 tgaaatactt tttagatagc acaacagcac tgtggttgaa aggtgcgctg attggcaaac
      361 ctgccgctgt attcacctca actgggtcaa tgcatggcgg taatgaaagc actttactga
      421 ccatgatgct gccgctcatg caccatggca tggtgatggt tggcttaccc tactctgaac
      481 ccgagctttc aagcacacaa actggcggca ctccttatgg tgcaagccat gttgccgggg
      541 ccatggatga taaaccgtta agtgctgacg agcgaaaact atgtatggcc ttaggcaagc
      601 gcttgggcga aattgcgtta aagttgaacg cataaatgcc ggtggtttcg gataataagt
      661 gcatgttaat caataaaaag aatcgagcga gtatcaaatg cgtttagcta acgaggcatt
      721 acaatttctg cgaagcaccc gtagtggtgt actttccact ttttctgcaa aatatgcagg
      781 ttatcctttt ggctcggttg cgccttttgt gctggatcat tctggtcaac cagtgatttt
      841 aatcagtacc attgcagaac acacccataa cattgttgcc aacccaaaag tgtctttatt
      901 agtgtttgcg ggcgatgacg atttacaggc aaacgcacgg ttaacactga ttggtgaagc
      961 caaactgata gataaacaag atgtcgattt acgcgcgcgt tatttacgct atttaccaca
     1021 ggctgcgagc tattttgata tgcatgattt taatttctat cgcattgaaa ttgcacatat
     1081 acgctatatt gccgggtttg gtcgcatggg ctggattgat ggcactgaat tgacgcacac
     1141 ccaactgtta gctagtagcg cgcttgctac ccaagaggct tcaattatcg agcatatgaa
     1201 taccgaccac gtacatagct tagtggctta ctgcaaacat tttcaccaaa ttgaagcaac
     1261 gcacgcacag atgctgggta ttgaccgtga tggttttgat gtaaaagtaa cagacgcaca
     1321 acacaacacg caggtgttac ggtttcattt caaccagcct attgaagacg cacagtcggc
     1381 tagagtagct ttagttgcca tgtcaaaagc ggcaacaaca tgattcaaat actaagactt
     1441 ggtgcctcca ttagcttaat ttcgctcatt ttattgtgcc tgtgttggga ggcgttttta
     1501 gccccactta agcccaatgg cagcatgctg atactcaaaa ccgtgccact gttactgcct
     1561 ttatttggtg tgctgcatgg taaacgctac acctaccaat gggccagcat gtttatactg
     1621 ttttacttta ccgaaggcgt ggtgcgcgcg tggtcagata ctggcttatc ggccaattta
     1681 gcgctgattg aggtagtact ttgtgtcatc ttttttgttt gtgcaatttt ttatgcaaaa
     1741 ctcacgcgaa agccagttca ataatttata atcaggctca tgatagctca ccctttaaaa
     1801 tcacgtaaaa aattacgcac caatcttgta tgaaaattct tctctcaaac gatgatggct
     1861 attttgcacc tggcctcaat attcttgcag aacatatcgc gcaactggct gaagtgatgg
     1921 tggtagcgcc tgagcgcaat cgtagtggcg caagcaattc actcacgctg gatcggccgc
     1981 taagtgtgcg taaggcaagt aacggctttt attacgtgaa tggtacccca actgattgtg
     2041 tgcacattgc cttaactggg ctgatggaag acatgcccga catggtgatt agcggcatta
     2101 acgatggtgc caatatgggc gatgatacga tttactcagg taccgtcgcc gccgcaatgg
     2161 aaggctattt gctgggcatt ccatcaattg ctgtttccat gtcgcaacat aattcaacgc
     2221 actttgaaac agccgcaaaa gtagcggttg agctagtgca gcattaccaa aaacacggtt
     2281 ttagttcacc cactttgctc aatgtgaatg tgcctgatat tccttacaat gaactgcaag
     2341 gcactgtggt cacacgattg ggtaagcgcc acaaagctga acctgttgtg cagcttaaaa
     2401 caccgcgaaa tgaaacggta tattgggtcg gtgctgctgg cctgccgtac gatggcggtg
     2461 aaggcacaga tttttatgca gttgccaatc acaaagtgtc aatctcgcct attcaagtcg
     2521 atttaaccaa tcatgcgcaa ctgtcagaca tcaatgattg gctgaaaagc taagtcaatt
     2581 gaaatggaat gcgtggaaca cccaaattgg cgttaactag acccccaaca caaactggca
     2641 tcggcatgac atcgctgcgt acgcgcgagc gtatgctcac gcgccttcgt gatcaaggca
     2701 ttaaagatga agtggtgctt tctgctatct cagcgattcc gcgccatatt tttgtagatg
     2761 aagccttatc aattcgtgct tatgaagatg tttcattgcc aattggtttt ggccaaacca
     2821 tttcgcaacc ttacattgtg gcgcgtatgt ctgaggtatt gcgtaacggc aagccgctac
     2881 aaaaagtgct tgaggtgggc accggttgcg ggtttcaaac ggcggtttta tcaaaattag
     2941 ccaaagaagt gtattccgtc gagcgtattc gcccattggt gatgaaagcg cgcgggcatt
     3001 tgcgtgagct taaatgcatt aatgtacggc taggccattc agatggtgcg atgggtttac
     3061 cagaacacgc accgtttgat ggcattatcg tcaccgcagc ggccagccac attccacaag
     3121 atttgttaga ccaacttgca gtgggtgggc gtttagtgat tcctgtaggc gttgatgagc
     3181 aaatactggt gctagttgag cgcagaactg aggctgacta ccgccaaacc aagttagagc
     3241 ctgttaaatt tgtaccgttg ctcggtggtg tcagttaact tatgccttta ttgattaagc
     3301 cccttcgttt tattagtttt tgcgcattag tcatgacatt gacagcctgc ggtagcaacc
     3361 cgccagcacc ggtcacagac cgtacaccaa ccaagcccag caaacctgta gctaacagcc
     3421 aaagcaccaa cacaagcagc aaagccaacc gcccaactta caaacctggt gattggcggc
     3481 ctgataatta cacggtaaaa aaaggcgata cgctgtatag cattggcctt gagctaggtt
     3541 attactataa agacattgcc caagccaata atattggcgc gccctatctg attaaggttg
     3601 ggcaggtgtt aaaccttgct gcgttgaaag aaaaagttgt agtgactgag gtaaagcctg
     3661 aggcggcgac tggcacacaa cctgacccac aagaccagga tggcgtgatt atcacgccaa
     3721 ttggcgcaga aactgccagc cctaacgcca ccatcgaaag caccgaaacg ccagcagccg
     3781 ttgcaattgt cgccatcact gagccaaaag cgattcgtga actgtatagt gatgaagccc
     3841 ttaaaaagcc tctgccaacg ccaaaaccaa ttactgtaaa accaagcaca cccactgaag
     3901 taaagcttga caccaaacca gtagaagtaa aacccaccga agccaaacca gccgtggcac
     3961 ctacgctaaa acctgaaaca aaaacgaccg ctgaatcacc tgaagatatg gaatggtcat
     4021 ggccaaccaa gggcaaagtg gttggcaact ttagtgaagc cggtaacaaa ggcattgata
     4081 ttgccggcag ccttggccaa gcgattacgg cggcggcccc cggcaaagtg atttacagcg
     4141 gctctgattt acgcggttac ggtaaccttg tcattattaa gcacaatgcc acgtatttat
     4201 ctgtgtacgc gcacaatagc gccattttgg ttaaagaagg ccaacaaatt gcgcgcgggc
     4261 aaaaaattgc cgaaatgggc aacaccgata gcaacacggt aaaactgcat tttgaaattc
     4321 gccgccaagg caaatcggtt gacccaagca agtatttggc cgcaaactaa cgttacaact
     4381 caggaggcac tatggcaaaa aatccacgtg aaaaagttga ccccaataaa tacttaaaaa
     4441 acaaagatat acaagctgaa agcaaactca gcgttttaat tgccgtcatt aaaatgggtt
     4501 tggtgctggc aggcattgcc ggcgttgcga tggagttttt ccgcgatggc ggctggttaa
     4561 aaacggccct tagttggctg tttgatagca ccgaacacat gatgtttatc cccgtaatta
     4621 tttttgcgat gtggtttctc aacaaaatat ttagcgccgc gggcaaaggt gaaaccaaaa
     4681 aaagtggcga ctttcccatg tatatcatga tggcgattgg cgcctattat atttttctct
     4741 acctcaccgc cgcatagaga atttttgtag cgattcaact aactaaaccg tcattcccgc
     4801 gcaggcggga atgacgaatg agaataactg acttcattat cggaatattt aacggaactg
     4861 ctgtcaatca agcccaaaaa tccccgagaa aaaaataatg ccctggaagc ccttattcat
     4921 aaggctcaca gggcatctaa aaaggttgcg gcttaaattt aaactttaat tcgctttttt
     4981 agcctcttct ttgtaataag cgcgcacggg cgtcatatca aaatcgcgcg cccatttaat
     5041 aatttcatca aacgctggcg cgccaatttc accttgctgc gcatgaatgc cggcttgctc
     5101 gcgaatcacc aaaatgcctg ggatttttgt cacttcaaaa tactcggcaa tttcaggcgc
     5161 ggcttcagta tccaccatgc caaataaaat atccggatta ttatttgctg ccgcctcaaa
     5221 aatcggcgtg aatgaaacac atggctcgca ccacggcgcc caaaaatcaa caatcacaaa
     5281 cggattgttc acaatcgtgt ctttaaagtt ttctttggta agggtaacaa ctgccatgat
     5341 ttagctttca attaaacggt ccgtcaaatc gaccagaagc gtgattttag cagagcgcta
     5401 ttatttagtt aaggtgtttt gttcaggtgt ttttttgtga ggggaaaaga agcaatcaaa
     5461 aaccacaaat aattagcgta atacatggtg atttaggtac ataaatataa ccaactttct
     5521 gccgagaaaa aaataatgcc ctgtgagcct ttatttacaa ggctcacagg gcatttaaaa
     5581 aggttgtgca taaaaacact ttagaaccac ttgatttaaa aagcaatgaa ttgttaggta
     5641 gttatattgt cgctaaacaa ttggctttcc ccaactttca ctaaaaaata attccgccag
     5701 tggtttgcgt gtgcgcgctg cggtttcgcg cgtcagtgct tcacccgtga ccgtggcaat
     5761 gtcagctggg tgtcccacgg taataaacgc cataagggta tattggctgg gaatattaaa
     5821 gctttcgcgc gctaggtctg ggttaaagcc gcccatttgg tgcgccatca ggccgagggc
     5881 ttcggcttgc aaacagaggt tttctgcggc ggcgccagtg tcgtattgtg cccagcggtt
     5941 gggttgctga ttgtggccaa ataagctgtc ggccgccacc aacaccaata cgggggcatc
     6001 tttggcccaa gcctgattac cgggtactag gcagtctagc gcttgctgcc acgcactagc
     6061 atccgtattt ttatcccaca caataaagcg ccaaggttgg tcgccaaagc acgaaggcgc
     6121 ccagcgcgcg gcttcaagca ggctgatgat ttgcgtttgc gtgacgggtt ggctggcgtc
     6181 ataagcgcgg ccgctccagc ggttggcaat gagttcatgg attggcgctt gggtgttggc
     6241 aggttttttc atgagattgt cgcccttaaa aaatttaaac ttctaaaaat ggataatcgg
     6301 tatagccttt ttcagtgcca ccatagaagc tttttgcatc agccacattg agtggcgcat
     6361 ctttattaaa gcgctcgact aaatctgggt tggcaataaa gggtacgcca taggcaatgg
     6421 catcggcatg gccgctggca atagcagcat tgccgcgtgc tttgtcataa cttaaattgc
     6481 caatgtagcc atttttaaag cgctttctaa aggccacaaa atcaaacgga tcagccttgc
     6541 cgccaccgtg aataccgcct tcaaccgcat gcaaatacgc caagttaaag cggtttagcg
     6601 cctctgccac gtattcaaac aaggcttgcg gattactatc gtgcatgtcg ttaaatggat
     6661 taacgggcga caatcgcaca cctactttat cagccgctgc ggtttttacc accgcatcga
     6721 caacttcaag cagtaagcgc gtgcggtttt caacactgcc gccgtagttg tctgtgcgtt
     6781 tattgctgcc atcgcgcaaa aattggtcta acaaatagcc attggcggcg tgcacttcaa
     6841 cgccatcaaa acctgcagcc agtgcggctt tagtggcgtg cacgtagtct tgcacaatcg
     6901 cgggcaattc gtttgcctca agcgcgcgcg gcgtaacgta atcttgcagg ccttgatagg
     6961 taaacgcttg gccggcaggt ttgatggcag aaggcgcaac gggcaaggca ccattgggca
     7021 acaggcttgg gtgtgaaata cgccccacat gccagagttg aatgacaatt ttgccgccct
     7081 tggcatgcac cgtatcggta atctttttcc agcccgccac ttgtgcatct gtgtaaatgc
     7141 cgggcagcgc tgggtaaccg tgcgctaacg gcgaaatcgg tgtggcctct gtaataatca
     7201 gcccggccgt ggcgcgttgt gcgtaatacg tcacattcaa atcatttggc acaccatttt
     7261 cacccgcgcg attacgtgta agcggcgcca tcacgatacg gtttttgagt gcgatactgc
     7321 ccaactgaac gggtgagaat aaatcatttg ccattaaaaa ctcctattaa attgcattca
     7381 ttttttgact gataaaaagc atcaaaacca tcttatttta cgcccaacct tttttgatgc
     7441 tctgcaagcc tttatttaca aggctcgcag cgcatttatt tttcctcgca gttgaaaacg
     7501 cttatttttt gtcttttgca ggcgctggtt ttacgccttc aatcagcaaa gtaacttcaa
     7561 tatcgtcacc cactggcgca ccttcttttt tcatccattc atagccaaaa tctgatgcct
     7621 taatggtggt gcgtgcttca aaaccagaac gctcgccgcc ccaagggtct ttgccaaaac
     7681 ctaacacggt aaatggaaag ctcaactctt tcgtcacgcc atgcaaggta aattggcctg
     7741 ttaatacgcc ttcggtcgcg cttttagctt ccactttagt gctgacaaat gtaatttggc
     7801 caaacgcagc ggcatctaaa taatcaggtt ttttaatgtg gtcatcacgt tttgcatgtc
     7861 cggtgctgat gctggccacg ctaattctgg catctacgct tgagttggct aagttttcac
     7921 ggtcaatctt gatggtacct tgaatgtcgt taaagctgcc ggaagtttta gccactacat
     7981 ggcgaatgct gaaattggca aaactatgtg cggtgtcaat attgtaaagc tcaggcgcgg
     8041 caaatgcggt gctactaaaa aatacagctg aaactgctgc aatgagtgtg tgtttcatgg
     8101 ggttaccttt cagttaaata atgaaatcaa tcaaacttaa ttattcgaat taattatatt
     8161 actatatgcc aatattatta aatatttatg gtaaatcaaa ccataaaact tcagcctcag
     8221 ccatggcgat gagctcaatc gcagtttcag cttcaatttt tgcagcatcg cctgcggata
     8281 attgcacacc attacattgc acctcacctt tggcaatttg caaataagca cggcgattat
     8341 cctgcagggt tttgctcagt tgactgccag catccagcac gcttgcatac aaatcaactg
     8401 cttgctgaat ttgtattgac ccttcacggc cgtcgcttga cactactaga caccaacagt
     8461 tgcgcttttg ttcaggcaaa aagtgttttt ctgcgtagtt tggcggcaag cctaaaacag
     8521 atggcaatag ccagatttgc aataaatggg cttcatgttc agcagatggg ttgatttcgc
     8581 tatgcctcac acccgcgccg gccgacatat attgcacatc gtccgcgcga atgatcgagc
     8641 cgttgcccat gctatcttgg tgcttgagtt cacccgccag catgtaagtg ataatttcca
     8701 tatcacgatg cccatgcata ccaaagcccg tgcctgcggc aattttatct tgattgatca
     8761 cgcgtagcac tgaatagccc atgtgcgccg catcgtaata atcggcaaat gaaaaagaat
     8821 gaaagctctc cagccaatca tgcttggcgt aaccgcgatc ttgagatttt cgaattgtga
     8881 tcatattatt tattcaatat agttgaatta gttatagcat gataaactta gaccatagac
     8941 attcataaca aattattttg cattacttat tcaaattatt tgattattat ggcgcctgca
     9001 tatgaccctt aaaatttcta tcgaagcact agaagtgatt gatgcgattg cgcgcaaagg
     9061 cagctttgcc gcagcggctg aatcgctttt tcacgtgccg tctgccctca cctacaccgt
     9121 gcgcaaactt gaagaagact tgggcgtcac tttatttgac cgtagcggcc atcgcgcgcg
     9181 cctcacggat gctggcacag aactattaaa tgaaggccgc catttgctcg atgcagcgct
     9241 tgcgctcgag tcgcacgtga agcgcgtggc cacaggggtt gaaacccaca tcggcattgc
     9301 gataaacgat ttgtttaata tgcagccgat atacaacttg ttgcaagcat tttacgcaca
     9361 agggtttggc acgcgcgtaa aactgacgcg cgaagtgttt ggtggcagtt gggatgcact
     9421 actttcaggc cgtgctgata tttcaatagg cgcacctggc gaagcgccat cgggcggtgg
     9481 ctatgcaacc aaattgctgg ggcatttaga gtttgtgttt gccgtagcac cgcatcaccc
     9541 gttggcgtca ttacaggaac cgcttgaaaa tcagacgatt atcaaacacc gcgcggttgc
     9601 agccgctgat agctcacgca atttaccacc gcgcacgtct ggcattttat cagggcaaga
     9661 tgtgctcacc gtgccagata tgcaaagcaa acttgaggcg caaatgaacg gcttaggcgt
     9721 tggttattta cccatacagc ttgcaaatca ctacgcaacg caaggtaaac tcatcattaa
     9781 acaggtggca gaacctaaaa caattgcccc aacattttta gcatggcgca acaatcgaaa
     9841 aacaggcata ggcaaagcgc aacaatggct attaaagcgg ctagagcgat taacgttaga
     9901 agaattgctg aagaattgaa aaaaatctat cgcaattaca tgtagaatat tcactgatgc
     9961 gcctagctca tgaaacactt aacagattgt gactcaatac tggtcaacaa tcatccaacc
    10021 aacaagatta agcacatcat gaaaaattta accaaccaaa ttctggccgc tgttaatgaa
    10081 gtggtgctag gcaaagaagc acaagttaaa ctgtcgctcg cctgcattat tgctcgcgga
    10141 catttgctga ttgaagacca accgggcatg ggtaaaacca cgctggccca tacacttgcg
    10201 actgtgttgg ggcttaaata tcagcgcgta cagttcacca gcgatttatt acccgccgat
    10261 attttaggcg tttcggtgta cgacagaaat tcagccagct ttaaatttca ccatggcgct
    10321 gtttttacca atgtgttgct ggcagatgaa attaaccgcg ccacacccaa aacccaaagt
    10381 gccttattag aggtcatgga agaggcgcaa gttaccattg acggcaatac ttacccactt
    10441 acgcagccgt tttttgtgat tgccacgcaa aacccagtgc aacatattgg aacttttccg
    10501 ctgccagaat ctcaactcga ccgcttttta atgcgcatca gtttaggcta cccagaccac
    10561 gcctctgaac gcgctttatt aaaaaaccaa ggcgggcgcg ccattgccgg gcaaatcaaa
    10621 gcggtctgcg atgcgcgcac cctgattggc ttgcaacatg tggccagcaa aattcacgta
    10681 tctgatggcc tactggatta cctgcaagcc ttggtggaat atacccgcag ccattttgct
    10741 acggggctat cgccaaggtc tagtcttggc ttgttgcaat gcgcacgcgc atgggccatg
    10801 tgtgaggggc gagattttgt catcccagaa gacatcaaga ccgtattacc aagcgtggtt
    10861 gggcatcgtc tgcaatcaag cgacaatacg catgtagcgc agcaaatatt ggatagcatt
    10921 gctattccaa aactctagtg tttggaacgc agcatgcaag cacaattttg gccgctgact
    10981 ttttggaagc gtgactggtg gcaacagtgg tttcgcgttt ctgaaagcag caataacgta
    11041 gccgtattag gctcacggca aatttatatt gtacccacac gttgggggct gctttatgcg
    11101 gtgatggtgt ttctgctgtt gctcggctcc attaactatt cgttaagctt aggcttttac
    11161 cttacttttc tattaacctc tctgggttgc gtggccatgt ttcacacttg gctcaacttt
    11221 gcccatcttg aaattgaaat attaagcgcc aaacctgttt ttgctgggca agaagcgctt
    11281 gtccacatcc aagtcaaaga atataaaaac caacaacggc atagcattgc cgcgcatttt
    11341 gatgcagaat ctgccgtgct tcacacgatt ccagcacttg gggcacagtt atttgccgtg
    11401 cccattagca cgcgtcagcg cggctggttg agcctgccta aattggtttt ttataccgaa
    11461 ttcccactag ggctatttca cgcttgggca atagttcgcc atccaatgaa tatcatggtg
    11521 tatgcaagcc ccagcgaaaa tcctagccca gtggtgccgg ttgtcagcgg gctggccagt
    11581 gcatcgccgc atgccagcca gcaaggtgac gatgatttta ttgggcataa aagctatcaa
    11641 atcggtgacg cgccaagccg ccttgattgg aaagcttcaa gccgcggcgt tggcatgctg
    11701 accaaacgct atagcggcaa tgcacaagaa atcctctggc tcgattgggc gaccacccaa
    11761 ggattaccca tggaagagcg catcgccatg ctgacaaaat ctgtgattga tgcccatagc
    11821 acacaactta cttttggcct aaaactcccc ggcaataccg ttcagcctgg ccaagatgaa
    11881 gcgcattatc atcgctgcct acaggtactg gcgttactat gaacacgtca ccaaagccgc
    11941 agcatttcaa atggttgctg gcaacactgg cgcttattct gctgttgcat gcaggcaatt
    12001 tgccaatctg ggtggtcatt atcagcgccc tgtttggcat atggcgcttt tttattctta
    12061 gtaaaaactg gcccatgcca aaactctata tactggcacc catcaccatt ggcattggct
    12121 tgggcattat ttttaccttt ggtggcatgt ttggtcgcga taccagcctt gcactgctcg
    12181 tttcaatgct ttcccttaaa ttattagaaa ccaaaaccaa gcgcgacttt tacttagtcg
    12241 tcattttggg ctattttgtg gtgggcaagt tatttttgtt caatcagtcc atgttcacgt
    12301 ttgtgctgag cattgcgcct ttagctttac taactgcggc ccttgttcaa attaactcaa
    12361 cccacgcgca gccaagcact tatttactaa aatatgcggc aaaaatgttg ctgcaggcga
    12421 ttccgctgat gctggtattg tttgtgcttt ttccgcgtat tcctggccca ttgtggagtt
    12481 tgccgcaaga tgcgcacagt aaaattggtt tgccgggcct gagtgattcc attaaattca
    12541 accaagtaag ccaaaatgcg caagacaaca gcgtggcgtt tcgggtgaag tttaaaggcg
    12601 atgtccccgc gcaacaacag ctttattggc gcggcccagt gttgttgaca agcgaccagg
    12661 atgaatggaa tatcagcaat caagcgggct atattaacca agaaccgctc actatcggtg
    12721 gcaaacccat tgaatacagc atcaccttag aaccacacaa gcaaaaatgg ctgctcatgc
    12781 tcgatatgcc tagtcaggtt cctgattttg caacgtttac gcatgattat tctgtggttg
    12841 ccaaagagcc ggtttatacc cgtattcgct acgatgcgac ttcatacgcc cattatcgct
    12901 tgggggcaaa ccgccttacc cagcgcgaaa aaaacctgag tttacaacta gaagaaggtg
    12961 tcaacccacg caccattgcg cttgccgagc aatggattga tttaaatgca gaagataccg
    13021 tgaatcaagc gcttaaacta tttagagaaa aagattttta ctacacgctt aacccgccga
    13081 ttttaggtaa agacccgatt gatgactttt tgtttaacac caagcgcggc ttttgcgagc
    13141 attacgcgat tagttttgtg gtgctgatgc gggcggcagg cttgcccgcg cgcgtggtca
    13201 caggttacca aggcggagaa cttaatccgc atgataattt tttaatcgtg cgtcaaatgg
    13261 acgcccacgc ctgggctgaa gtttggctca aagaccgcgg ctgggtgcgc gttgacccca
    13321 ccgctgcagt agcccctacg cgcatagaac gcggcattgt agaggccgcg cgcgttgcca
    13381 gacccgcacc tgggcaaacc aaagctgaaa ccagcaactt acccgtggcc gcacgcacaa
    13441 aagattaccc ttggctgcat caaatgatgt tgcgcttaga tagcatcgac aatggctgga
    13501 accaatgggt gataggctac aaccaaaaga aacagcaagc cgtattgagc caaattacgg
    13561 ggcaaaacgt gacggtgaac acattactga tttggctaat ttgcgcgttg atgctgctgg
    13621 gtggcttgac actattcatc attttcagaa aaaaccggca aaaacagagc cgcacccagc
    13681 aactttacgg gcagtatatt cgcgcgttaa agccttttga tttagcccca aaaccacacg
    13741 aaggcgcgct agagtttgcc gagcgtgtgg ccatagcact gccacagctc aaaaaagaag
    13801 tatttaatat tgcagaaagt tataatacgt tgcaatacag tcgttttagc agtctgccag
    13861 acacgacgct gctcgctgac tttgaaaaga caattcagca atttaaccca aaaagcacgc
    13921 aacaatctca tgcaaaacac taaccaacca ttgacggatg aaaccggaat gcgcgccgcc
    13981 atagaactgg caaagcttgc ggctttgcgt ggtgaagtgc cagtgggcgc gattgtagtg
    14041 aaagaaggcg tcattattgg ccgcggcagc aatgcgccca ttgaacacca tgacccaagc
    14101 gcacacgcag aaattatcgc catgcgagaa gcggccgcat tgcttggcaa ttatcggctg
    14161 gtcggatgca cgttgtatgt caccttagag ccttgtgcca tgtgtagcgg cgcgattcag
    14221 catgctcgca tcgcaaggtt ggtctatggc gcacaagacc ccaaaacagg tgcttgtgga
    14281 agcgtggtag acttaatggc cgaaacaaag ctcaaccatc acaccgaagt ggtcagcggc
    14341 gtattagcgc aagaatgcgg cgctttactt tcagattttt ttagagcccg ccgactagcc
    14401 aagctgtaaa tacaacacaa cgcttcaaga cagtacgcat aaaaaaactc gccgaagcga
    14461 gttttttatt tgagcgctca aactgcaata ttagtcatcg ccacccataa acgccatcaa
    14521 caagtgcaat aagttagtaa acatattgtg caagctgatg taaatgctca tggttgccaa
    14581 gatgtaattg gtttgaccac cgcgcacgat ttggttgatt tcatacaaaa taaaccctgc
    14641 cattaacatc acaacgaccg cagaaatggt gagtgataac gctggaattt ggaagaaaaa
    14701 gttagctaaa attgctgcaa ttaccaccac aaagccaatg gttaaaaatt tacccatgaa
    14761 gctgaagtcg cgtttagtgt tagaggccac gcctgctaaa gttaaaaaag aaatagccgt
    14821 accacccgca gccaagccga tgagttgtgc accattactt aaattaagtg cgtattgcaa
    14881 aattgggcct aacaacacac ccataagaaa ggtgaatgca aacagcaacc aaacacctgc
    14941 tgcattattg gcatttttgg caatcagcca tgtcatgccg aacacggcag caaaaatacc
    15001 aataaacacc ataatcgggc tagaggccat catccatgca aaattcatgt taatgcccac
    15061 cactgcacca atcacagttg gaatcatggt catgcccaac atcgcataag tgttacgcaa
    15121 cactttattt tgcgcttcaa ccgcggtggt ttcacgacct aaaacttgaa tattttctga
    15181 cattgttatt tcctcaatta aaatatgcct aacaggcaac gttgctaaga tagcgatttt
    15241 tgcaataagt ttcaagtgtt aatgcaggtt ttacacaata cgccagtgcg aaacccatgc
    15301 gatattaaac ccatacactt gctaaagttg tgctcaaaac ttgccgaaac aatcaattaa
    15361 aaatgtcttt ttaagcaaaa tttacacatc aacctttttg atgcctctgc gagcctttat
    15421 ttataaggct cgcagagcat aaattttttc tcgggcattt ttattaataa atacagtcgt
    15481 ttttaacgcg ctaaaatcaa tgagaaatca taccaaaacc acggcaacat tgccgcccaa
    15541 catcagacaa ataattcacc ttcggtaatg ccaatttgcg ccacttgatg cacggctttt
    15601 acaaaatcac cattttcgtg cagtgcatct aaggttggcg gttgcctgtg gccgtgcatg
    15661 cgcgccaagc ggcttaaact ttgcgctgaa attgtccact ttaaatttgc gagtaattca
    15721 tcggccatgg cgggctgatt acataacagc accatatcgc agccggcatt aagtgctgcc
    15781 agtgcacgtg tggtgacatc gccacctgca ctggcggctt ccatacttaa atcgtcgcta
    15841 aaaatcacgc cattaaagcc tagccgctcg cgcaacactt tttgcagcca tttcggtgaa
    15901 aacccagcgg gtttatcatc cacgcttgtg taaatgacgt gcgcgggcat aatcgcttga
    15961 atgccgtcat caatcagcat tctaaaaggc tgcatatcat tttgcgcaat tttgtcaaaa
    16021 ctgcgttcat ccacgggcat actcacatgc gtatcgcccg ccacaaaacc atgcccagga
    16081 aaatgcttac ccaccgctgc catgccgcct tttttaaggc cttgcatcaa agcaaaagcc
    16141 aagtcattaa tggcctgtgg gtttaaatga aacgcgcggt caccaatcac caagctatcg
    16201 ccgtaatcca tatccaatac cggcgtaaag ctaaaatcca cgccgtgtgc gcgtaactct
    16261 gacgctaaaa tccagcctgc ttccgtagca agctctttgg cctttttagg gtttttatcc
    16321 catattttgc caaattcacg catgggcgga attttagtaa agccttctct aaaacgttgc
    16381 acacgtccgc cttcatgatc taccgcaatc aataatgttg gctgacgcac cgcatgaatg
    16441 ctgtctgtga gtgcttttaa ttgtcggtta ttttcataat
//
LOCUS       DAOQSI010000026        16460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_2610_length_16460_cov_5.859738, whole genome shotgun sequence.
ACCESSION   DAOQSI010000026 DAOQSI010000000
VERSION     DAOQSI010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 16460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16460
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2610_length_16460_cov_5.859738"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="PKL42_03105"
     CDS             complement(<1..1176)
                     /locus_tag="PKL42_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNU66091.1"
                     /translation="MHHSQLNLSSATRQNMQRLMMLRGVVILCLVVVMVGLKQASIPL
                     HVLPLTIGLGILCALSVLAWWRLQSSAHTTQRELLFQLMGDIAVFTLIFYFSGGYSNP
                     FIWMFLIPITVAAVALRAVYAWLVAALSITSYTLLMFFHIPLSHLHMHMRGDTQLDIH
                     LVGMWLGFVISAIIVAIFISRIGQNLRDYDKKMAEVREKALESERMLALGTLAASAAH
                     ELGTPLATMAVVSKELAQEFSHQPEALSQLDIMRTQIMRCKEILSSITQNAGKTRADG
                     GQGILLRDFLEEAIQRWRDTRPATELVVAFANHTVNPLIFSDRTLTQAIQNMLDNAAD
                     EFADRVLLNAHWNEKMVTIEIRDFGKGLSQEVKQQLGKPFFSSKNDEGMGLGVFLTQT
                     "
     gene            1346..3568
                     /locus_tag="PKL42_03110"
     CDS             1346..3568
                     /locus_tag="PKL42_03110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNU66092.1"
                     /translation="MKKIALAVMLACNSGLLAAEETAIVNVAEQVQADTAEAPITQLE
                     PMQIRAESIGSSLLLDTPSTAGSRLDLTPRETPASVTIVDRNLIEQRGATNTQEILKS
                     VPGVLAASPPGSAGSVTYRGFGSSSLTQMFNGITVQYDAIAARPVDSWIYDRVEVVGG
                     PSTFLFGAGAVGGSINNITKTASKSGDFTQARASYGSFDTSQLSFGLNRQLGDDVGVK
                     NYVRADINRTNSNGYVDGNEREAWSGAFSLLTEFTPNLSHTLALEYQNEQDDRVYWGT
                     PLLRPASGPIVGNGKIADGTRFKNYNSADGIYEQTVKWGRSIIDFKLNDTTSIRNTFY
                     HYDALRDYRNVEVYKYNVTNTAVVRSSPLLQRHDQEVDGDRVELSYKTDLGGMKSDWA
                     AGFDYNVNRQTRFPRSLTLNVSTVNPYNFTTEKFFDIPGMVRGFNPQRTNRVETLAFF
                     LENRTKFTDQFSLVTGLRHDKIDLSVKNHQVATATNPAYFDRKYTPTTGRVGLIYDFT
                     PTANAYVQYSTAADPPAGILTTAAFTQVRDFDLTTGKQIEVGSKFDFLEGRGSATIAA
                     YKIERKNLATPDPGNPGTTIPVGQQSSRGIELAASMKVTDDLTLQGNYSLVDAQYDKF
                     TQVVAGKAVSRVGNTPTNTPTSIANAWLTYNITPTLQAGADVRYVSSRYGDVANTFGD
                     ASYTLFGAFASYKLPKYGTLTARVRNLTDEVYAASVTGDQMFYLGAPRSYEFGWQAEF
                     "
     gene            3640..4932
                     /locus_tag="PKL42_03115"
     CDS             3640..4932
                     /locus_tag="PKL42_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66093.1"
                     /translation="MSGFSGDHSLAVVSLDNPVAEAGRLPRLRTLPNGDALMSWVEPK
                     GQGHALKFGVVHDGRWVRQGEAAQGENWFVNWADFSSVVAIDENFWVAHWLAKQKGGK
                     TYDYDVSLAISHDAGQTWREVGHPHRDGTAAEHGFATIFKDLDGAGIVWLDGREHVKK
                     EDKAKYPGKSGNFNLRYTHINRDGTMTDEQVLDNNTCTCCWTAVASTPEGAIAAWRGR
                     TDDEIRDNRVALLRDGQWRPPAPLGAEGWKIAGCPVNGPSISALGNKIVASWFTAEGD
                     RPRVRAAFSEDGGVTFGKPIEIDDISPLGRIGLTWRDSQSAVVSWLSAPEVEQKKSVL
                     KVAVIYTDGRVEPTRQLAEVSSGRDTGVPQMVSTADGLLFAWTNPAPQYGITTKLLRH
                     ADLKLAVQNVSKDKRQQKLAMLNHKFTINASICTSKHE"
     gene            5366..6037
                     /locus_tag="PKL42_03120"
     CDS             5366..6037
                     /locus_tag="PKL42_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNU66094.1"
                     /translation="MYENRIEKLVGLVLVVAIHVALLYVAMSYKLIPPPQEALTLMVN
                     LIKPPPPKEEPPPEPPKPTPPKEVKLIKKQKPIELPKPAPVLVAEAPVVAPTEPVAPP
                     PPPEPIQETPPPEPVVEAPPPKPAAPVQITGDMLQCSQKSEPNYPPASRRMGEQGSVK
                     LRLELDETGHITSAKVIESSGHTRLDNAGLATVKNWRCQAATRDGQPVRAVALQTFDF
                     ILEGN"
     gene            6063..6776
                     /locus_tag="PKL42_03125"
     CDS             6063..6776
                     /locus_tag="PKL42_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNU66095.1"
                     /translation="MANQLGFANFIQNVDSVGKAVLVFLLILSIASWYLIFTKSFANW
                     LEAKRAKAFLEKFRDVTSLEQLNSILRQGKPDNAFANLATEGLSALRNAGSFGSQKLA
                     GAGGISEFMTRAIRNGIDQESARSENGLTILASAGSASPYVGLFGTVWAIYHALVAIG
                     LSGQGTLDKVAGPVGEALIMTAIGLAVAIPAVFAYNAFNRRNRMWLAKLDAFAHDLFV
                     MLTVSNTENNQAKPTGNAS"
     gene            6773..7192
                     /locus_tag="PKL42_03130"
     CDS             6773..7192
                     /locus_tag="PKL42_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNU66096.1"
                     /translation="MSFGSFNSQQHAVKAEINVVPLVDVMLVLLVIFIITAPLLTHSV
                     KIDLPKASSNPNITKPDHVELAIREDGSLYWNGELVQKESLAARFELEAKKEPQPELH
                     IKADGTSKYEKIAQVMSIAAKTGLTKIGFVTDPSGVQ"
     gene            complement(7258..8538)
                     /locus_tag="PKL42_03135"
     CDS             complement(7258..8538)
                     /locus_tag="PKL42_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HNU66097.1"
                     /translation="MNNNNLIDVSQLRVGLYVHLDLGWMDHPFTFSNFKVKDETQIAS
                     IQKIGLKKLRYDPKRSDCEPLPIADLSIEDESIANQEVVAPPVPADTPLLQPVPQQVR
                     LKQLHHAMDESEKKFLVACDVVRKATNNILATPQTSIEQAALTVDDMVETALMEGDVA
                     IHALNGNRSSDAHYLHPMNVTVLSLMMAKSIEMSKEDARLLGMAAIFHDIGKIEISGK
                     VLSKQAPLTKQEQLHFEQHSEIGARMAKEVGLPARVGKIILQHHVHADGTGYPKQIKP
                     ELTDPLANIIALVNWYDNLCNPNDHTVAKTPYEALALMYTHQRNKFDSNLLKHLIKTV
                     GIYPPGSTVQLSTGVYAIVVSVNPNKPLRPYVMLHDPLASRLEPNIIDLRDEPNISIS
                     QCLRPNQLPKDALDYLNPRKRISYFLDADIPHET"
     gene            complement(8528..10456)
                     /locus_tag="PKL42_03140"
     CDS             complement(8528..10456)
                     /locus_tag="PKL42_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNU66098.1"
                     /translation="MKNLQDFKPYHLVSENDSFENLGQPFTGDRLRTSQLSNAVQNAL
                     EKHWVWVDINYHSNTGTSQTQAASSQDGLQSATAFSNSAYHLAKIAFENASEGMMVMD
                     ANANILSINKSFTAITGFNQQEVFGTVPKALQVTEREAWRHLQLDGQWRSELLNHRKN
                     GEAYQERLTVNAVKNQQDETIYYVGVFADITEAKHSQNRLHELVNHDPLTGLPNRRLF
                     NELLEHAIKRAEREHHQIALLFVDLDRFKAINDSLGHQVGDKLLYEVSKRIHHAMRDS
                     DIVARLGGDEFLVMMDMIKHPQDAALIAQKIIYALQVEFFIDGKEIFIGASVGISIFP
                     KDGSDVDSLIKAADIAMYQVKNRGKNNHCFYSEDLSKNAVERFTIESQLRHALERNQF
                     EMYYQPQISLLTGDIIGAEALIRWRHPDLGLVSPARFIPIAEETGLIVQIGEWVLRQA
                     ALQAIQWINDGYTMQWISVNVSGVQIMRSNFYDTVYGILIETNCNPNILELEITESTV
                     MQNTEFVIDTFNNIKQLGVRVAIDDFGTGYSSLSNLKRLPLDKIKIDQSFVRGLPDDL
                     DDAAITNTINAMACSLGFTVIAEGVETLAQATFLKNMGCLEAQGYLYSKPVTAAEFTK
                     LLAISTKKYHAKGNTHEQ"
     gene            10587..11156
                     /locus_tag="PKL42_03145"
     CDS             10587..11156
                     /locus_tag="PKL42_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66099.1"
                     /translation="MKTNATKKRWQIAVALTMIYGAIFGINSLQAKENGVVQEANVKQ
                     PPCPIAQAQDHRQQPIAFKQHMFDDGAQDLAVIAVDEQQGRITKRITYNQHQEAGCHF
                     SAVAIARGGDWGWLMVWADAEKIYYTRLDGEALVFVPPKKLPISQVTLIEFLLDTAQP
                     TMRVQTMQGKTQLLTSDDEGRNWQLLPDQ"
     gene            11442..14027
                     /gene="clpB"
                     /locus_tag="PKL42_03150"
     CDS             11442..14027
                     /gene="clpB"
                     /locus_tag="PKL42_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HNU66100.1"
                     /translation="MRFDKLTTKFQQAINDAQSLALGADNTAIEPQHLLLALLNQEDG
                     GTASLLSRAGVQLNPLKTSLNTAINNLPKSPDASGEVAISRDLNNLLNVTDKFAQKRG
                     DSYIASEMFLLALADDKGEVGRLLKQNGLTKSALEAAITAVRGNENVNSQEAEGQRES
                     LQKYTIDLTERAKLGKLDPVIGRDDEIRRAIQILGRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPDTLKGKKVLSLDMAALLAGAKYRGEFEERLKSVLKELAQDEGQTIVF
                     IDEIHTMVGAGKAEGAMDAGNMLKPALARGELHCVGATTLDEYRKYIEKDAALERRFQ
                     KVMVDEPTVEATIAILRGLQEKYELHHGVEITDPAIVAAAELSHRYITDRFLPDKAID
                     LIDEAASRIKMEIDSKPEVMDKLDRRLIQLKIEREAVRREKDEASHKRFELIEEEISK
                     IEKEYADLEEIWKAEKAQVQGSAHIKEAIEKVKFEMEEATRKGEWQKVSELQYGKLPQ
                     LEAQLKQASSAEAESGQVKHKMLRTEVGADEIAEVVSRATGIPVSKMLTGEREKLLTM
                     EAKLHERVVGQDEAVRLVSDAIRRSRSGLSDPNRPYGSFLFLGPTGVGKTELCKSLAN
                     FLFDSEDHLIRIDMSEFMEKHSVARMIGAPPGYVGYEEGGTLTEAVRRKPYSVILLDE
                     VEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFKNTVIIMTSNLGSQMIQSMSDQDYQVV
                     KLAVMGEVKTHFRPEFVNRIDEVVVFHALGEANIKSIASIQLQYLAKRLAAMDMQLKV
                     TDAALAEIASAGFDPVYGARPLKRAIQSEIENPLAREILAGNFMAKDTVQVDYKAGRM
                     QFSKV"
     gene            14140..14661
                     /locus_tag="PKL42_03155"
     CDS             14140..14661
                     /locus_tag="PKL42_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66101.1"
                     /translation="MNKQSLMLSTVLLTALLAGCASTAPREVAVDRLTPEEVAQITPA
                     ATPTLSLDEIVSLTQQKVSSDEIIAKIKASNSQYDLTASQIVDLSKQGVDSKVLDYIQ
                     TEREKARLNSLADEINKREKANKEAQEKLKQQAWRNRYDPFYDPFYHPFYGYGPYWGS
                     HFYLNNHLHRRRR"
     gene            14711..15130
                     /locus_tag="PKL42_03160"
     CDS             14711..15130
                     /locus_tag="PKL42_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNU66102.1"
                     /translation="MRQFSEQVTIKTAGRKLYDITPDVLQLTKKAAIQTGLLTLYIQH
                     TSASLLINENYDADVLVDMEAFFNRLVPDGDKLFIHTVEGPDDMPAHVRSALTQTSLS
                     IPIMQGRPALGQWQGIFLYEHRHVAASRRVILHAIGD"
     gene            15133..15504
                     /locus_tag="PKL42_03165"
     CDS             15133..15504
                     /locus_tag="PKL42_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HNU66103.1"
                     /translation="MGKRGRKLSPAEWQIYQPYFSPEVLHYARIVDGHTPFWLRPSMC
                     AVVLQQKIYFRAGYYQPSTLQGIALLGHELTHVEQFLQGMTIWHYLWECRHGYVKNRY
                     EVAAYAKGAMISYDVAKRGII"
     gene            15552..16049
                     /gene="lysM"
                     /locus_tag="PKL42_03170"
     CDS             15552..16049
                     /gene="lysM"
                     /locus_tag="PKL42_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HNU66104.1"
                     /translation="MGLFSFIKEAGEKLFGIGEAKAAEEVAKKDPTPVNVEAANKAAA
                     KAIADYVAKMGLTAEGLTIGFDGASATVIVQGMADTKETKEKILLCCGNVKGVEHVQD
                     QLGVIEPADESVFYTVVSGDTLSKIAKAQYGNANDYMKIFEANKPMLSHPDKIYPGQS
                     LRIPN"
     gene            16217..>16460
                     /locus_tag="PKL42_03175"
     CDS             16217..>16460
                     /locus_tag="PKL42_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66105.1"
                     /translation="MSTLLRALLALSVTSLLLVGCGEKEAAPVEATPAVEAPAPVEAA
                     PVEVAPAPAPAAKEPGGYEPTPEERVPALPLMIQRNR"
BASE COUNT         4665 a         3418 c         3970 g         4407 t
ORIGIN      
        1 ggtttgggtg agaaaaacgc ctaaacccat accttcatca tttttagaac tgaaaaaagg
       61 ttttcctagt tgctgtttta cttcttggct gagtcctttg ccaaagtcgc gaatctcaat
      121 agtgaccatt ttttcattcc agtgcgcatt cagcaatacc cgatctgcga attcatccgc
      181 agcgttatca agcatattct gaatagcttg cgtgagcgtt ctatctgaaa aaatcaatgg
      241 attaaccgta tgatttgcaa acgcgacaac tagctcagtg gcggggcgtg tatcacgcca
      301 acgctggata gcctcttcta aaaaatcacg cagcagtatg ccttgtccgc catctgcacg
      361 tgttttgccg gcattttgag tgatggaaga gaggatttct ttacatcgca taatttgtgt
      421 tcgcatgatg tcgagttgcg acaaagcttc aggttgatgg ctaaattctt gcgcgagttc
      481 tttgctaacc accgccatgg ttgcaagtgg cgtgccaagc tcatgtgcgg cgctggcggc
      541 aagcgtacct aaagccaaca tgcgttcgct ttctagtgct ttttcacgga cttcagccat
      601 ttttttatcg taatcgcgta agttttggcc aatacgcgaa ataaaaatcg ccacaataat
      661 cgcactaatc acaaacccta accacatgcc gacaagatga atatctaatt gggtgtcgcc
      721 gcgcatgtgc atgtgcaaat gtgagagcgg aatgtgaaaa aacatcaata aagtgtagct
      781 ggtgatactt aaggcggcta ccagccaagc ataaacagcg cggagtgcaa cagcagctac
      841 ggtaattggg attaaaaaca tccagataaa agggttgctg tagccgccgc taaaataaaa
      901 aataagcgta aaaacggcaa tgtcacccat gagttgaaac aataattcgc gttgtgttgt
      961 gtgtgcagaa gactgcaggc gccaccaagc cagcacgctt aatgcacata atatgccaag
     1021 tccaatagtc agcggcaaca catgcagtgg aattgaagct tgctttaaac caaccatcac
     1081 aaccacaagg caaagtatga ccacgccacg cagcatcatt aaacgctgca tattttgccg
     1141 cgtggcacta gataaattga gttgagagtg gtgcaaaata aattcgctta aatcatctga
     1201 taacaatcac attataaagc caacatcaca attatgccct gcgacaaatt gtcgcgcgac
     1261 aatatgccac attccgctta cttaaattaa gctctacaat tgcccgatat aaaaataatg
     1321 acaatatttt agagggatga aaaatatgaa aaaaatagca ctggcagtaa tgttggcgtg
     1381 taacagtggg ttattggctg cggaggaaac cgcaattgtg aatgtagcag agcaagtgca
     1441 ggcagatact gctgaagcgc ccattaccca attagagccg atgcaaattc gtgctgaatc
     1501 gattggttct tcactattat tagatactcc ctctacagcg ggaagtcggc ttgacttaac
     1561 gcctagagaa acgcctgctt cagtcacaat tgtagaccgc aatttaattg agcagcgggg
     1621 cgctacgaat acacaagaaa tattaaaaag cgtgccgggt gtgttagcag cttcgccgcc
     1681 tggctcggca ggttcagtca cttatcgtgg ttttggctca tcttcactta cgcaaatgtt
     1741 taatggtatt acggtgcagt acgacgccat cgcagcaagg ccagtagata gttggattta
     1801 tgatcgtgtt gaagtggtcg gtgggccttc gaccttttta tttggcgcgg gtgctgtggg
     1861 tggctcaatc aataatatta ctaagacggc aagcaagtcg ggcgacttta cgcaggcgcg
     1921 agccagttac ggttcatttg atacctctca attatccttt ggcctcaatc gtcagttagg
     1981 ggatgatgtt ggcgttaaaa actacgttcg cgccgatatc aatcgaacaa attccaatgg
     2041 ctatgtcgat ggaaatgagc gtgaggcttg gtctggcgca ttctctttgt taaccgaatt
     2101 tacgcctaat ctttctcaca cattagcgct tgaatatcaa aatgagcaag atgatcgcgt
     2161 ttattggggt actccgttgt taaggccggc ttctggtcca attgttggca atggaaaaat
     2221 cgcagatgga acacgcttta aaaactacaa ctcagccgat ggtatttatg agcaaacagt
     2281 caagtggggc cgctcaatca ttgattttaa attgaacgat accacttcaa ttcgtaacac
     2341 cttttaccat tatgatgctt tgcgagatta tcgcaacgta gaagtatata aatataatgt
     2401 cacaaataca gccgttgtga ggtcgtcacc cttattgcag cgacatgatc aggaagttga
     2461 tggtgatcga gttgaattaa gctataaaac agatttaggt gggatgaagt cagattgggc
     2521 ggctgggttt gattataatg tgaatagaca aacgcgtttt cctagatctt taaccttaaa
     2581 tgtaagcacg gtaaaccctt acaacttcac aacagaaaag ttttttgata ttcctggaat
     2641 ggtgcgtggc tttaacccgc aacgtaccaa tcgggtagag acgctggcgt tctttcttga
     2701 aaatagaacc aaattcaccg atcaattttc gttagtcacg ggcttacgtc acgacaaaat
     2761 tgatttatcg gttaaaaacc atcaagtagc cacggcaaca aatccagctt attttgatcg
     2821 aaaatatacg ccaaccacag gtagggtagg tttgatttat gatttcacac caacggccaa
     2881 tgcctatgtt caatatagta ctgctgcaga tcctccagca ggtattttaa caacggcagc
     2941 ttttacgcaa gtgagagatt ttgatttaac gacaggaaaa caaattgaag ttggaagcaa
     3001 gtttgatttt cttgaaggac gtggctctgc tactattgcc gcctataaaa ttgaacgcaa
     3061 aaatttagca acacctgatc ctggtaatcc gggaacgaca attccggttg ggcagcagtc
     3121 ttcacgtggt attgagcttg cagcctcaat gaaagtgacg gatgatctaa ccttacaggg
     3181 caattattca cttgttgatg cgcagtatga taagtttacg caggtggtag cgggtaaggc
     3241 tgtttcacgt gtgggaaata ccccaacgaa cacgccgacg agcattgcca atgcctggct
     3301 tacttataac atcacgccaa ctttgcaagc gggtgccgat gtgcgctatg tttcatcacg
     3361 ttatggggat gttgcaaata cttttggcga tgcttcgtat acattatttg gcgcgtttgc
     3421 ttcatacaag ctacctaaat atggtacgtt aacagcacgt gtgcgaaact taacagacga
     3481 agtctatgcc gcatcggtta caggtgatca aatgttttat ttaggcgcgc ctagatctta
     3541 cgagttcggc tggcaggcag agttttaatg aaagttattc attaagtgaa acaaaatact
     3601 aggctttcaa tcatcacatt atgttttgct gcttttttaa tgagcggctt ttcaggtgac
     3661 cattcgctcg ctgttgtttc acttgataac cctgtcgcag aagcagggcg actgccgcgc
     3721 ttacgtactt taccgaatgg tgatgcgttg atgagttggg ttgagcctaa aggtcagggc
     3781 catgcattaa agtttggtgt tgtgcatgat gggcgctggg ttaggcaagg tgaggctgcg
     3841 cagggcgaga actggtttgt gaattgggct gatttttcat cggtagtggc catagatgaa
     3901 aatttttggg tagcacattg gttggccaaa caaaaaggtg gcaaaactta cgactatgac
     3961 gtttcgctag caatttctca tgatgctggg cagacatggc gagaggttgg tcatccacat
     4021 cgagatggca ctgcagccga acatggattt gccacaatat ttaaagactt ggatggcgct
     4081 ggcattgttt ggttagatgg tcgcgagcat gttaaaaaag aggataaagc taaatatcct
     4141 ggaaagtctg gcaactttaa tttgcgttac acacatatca accgtgatgg aacaatgacg
     4201 gatgagcagg tactcgacaa taacacttgc acctgttgct ggacagccgt cgccagcacg
     4261 cctgaaggtg cgattgctgc ctggcgtggg cgcacggacg atgaaattag agataatcgt
     4321 gtggcactgc tgcgcgatgg tcaatggcga ccacctgcac cacttggggc tgaaggctgg
     4381 aaaatagcag gttgccctgt taacggtcct tccattagcg ctttgggaaa taaaatcgtc
     4441 gcaagctggt ttaccgctga aggcgaccgc ccgcgtgtac gcgcagcatt ttcagaagat
     4501 ggcggtgtca cctttggtaa gcccattgaa attgatgaca tcagccctct aggccgtata
     4561 ggccttacgt ggcgcgacag tcaaagtgcc gtggtttctt ggctaagcgc acctgaggta
     4621 gagcagaaaa aatccgtact taaagtagcg gtgatttata cagatggtcg tgttgagcca
     4681 acgaggcagt tggcagaagt gagcagtggc cgtgatacgg gcgtgccgca aatggtatca
     4741 acagcagacg ggctattatt tgcatggaca aatccagcgc cgcaatacgg aattaccaca
     4801 aagctattgc gacatgctga tttaaagtta gcagtgcaaa acgtaagcaa agataagcga
     4861 caacaaaaac tggcaatgct taatcacaaa tttaccatca atgcgtcgat ttgtacttca
     4921 aagcatgaat gaccgtcatt ttcagcataa tttttttata ggatataaaa aaagtaattt
     4981 ttagaaaacg ccaaaagaat aaccaatgag gtctaaaaaa tagtgttttt tatgcctaaa
     5041 tttacgcaaa cctttttaga tgctctgcaa gcctttattt acaaggctct cagggcagta
     5101 aaattttctc gagtgttttg gctgtgtgta agtattattt ttaagcgcct caagaaagca
     5161 ggttgttgag aaacattatc atttggagta atggcttgct tgtgatatat ttcagcagct
     5221 taatttttta taaatttaga tttttttaaa cggcaagcat tgcgtcacca agtgtcatga
     5281 caaagtgatc tggtaaagta tcttggcaac catctaaccg aaacaacgaa acattcaatc
     5341 aaaaacttga gaatgaacta aagaagtgta cgaaaaccgt atagaaaaat tggtaggcct
     5401 agtgctggtt gttgctatac atgtcgcatt actttatgtg gcgatgagct ataagctaat
     5461 tccgccccct caagaagcgc tgaccttgat ggtgaaccta attaagccgc cgccgccaaa
     5521 agaagagccg ccgcctgaac cgccaaagcc gacaccacca aaagaagtga agttaattaa
     5581 aaagcagaag ccgattgagt tgcccaagcc cgcgccagtg ctggtggccg aagcgccggt
     5641 ggttgctcct actgaaccag ttgcgccacc gccgccacct gagccaattc aagaaacgcc
     5701 gccacctgaa cctgtggttg aggcgccacc gccaaaacct gccgcaccgg tccaaattac
     5761 tggagatatg ctacagtgtt cacaaaaatc tgaacccaat tacccaccgg catcgcgccg
     5821 tatgggcgaa cagggttcag ttaagctgcg tttagagtta gatgaaacag ggcatattac
     5881 ttcagccaaa gtgattgaaa gttctgggca tactcggttg gataatgccg ggttggcaac
     5941 cgtaaaaaat tggcgatgcc aggcggcaac gcgcgatggc cagccagtgc gcgctgtggc
     6001 attacaaaca tttgatttta ttttagaggg aaattgaaat ggcacaagaa gttgggcagg
     6061 agatggcaaa ccagttaggc tttgccaatt ttattcaaaa tgtggacagt gtaggcaagg
     6121 ctgttttagt gtttttgttg attctatcaa ttgcaagctg gtacttgatt tttactaaaa
     6181 gctttgcgaa ttggttagaa gccaagcgcg caaaggcctt tttagaaaaa tttagggatg
     6241 tcacctcatt agagcagctt aatagcattt taagacaagg caaacccgat aacgcctttg
     6301 caaatttagc gacagaaggc ctcagcgcac tacgcaatgc cggcagtttt ggcagtcaaa
     6361 agttagcggg cgcaggcggt atttcagaat ttatgacgcg tgccattcgc aatggcattg
     6421 accaagaatc tgcacgcagt gaaaacggct taacgatttt agcttctgca ggttcagctt
     6481 caccctatgt tgggttgttt ggtacggttt gggcgattta tcacgcatta gtggccattg
     6541 gtttaagtgg ccaaggcaca ctagataaag tagcaggccc agtgggtgaa gcgcttatta
     6601 tgacggcgat tggcttggct gtggcgattc ctgcggtgtt tgcctataac gcttttaacc
     6661 gccgtaaccg tatgtggcta gccaagctag atgcctttgc acatgattta tttgtaatgc
     6721 tcacggtaag taatactgaa aacaatcagg caaaaccaac aggtaacgca tcatgagttt
     6781 tggcagcttt aactctcaac aacatgcggt aaaagctgaa atcaacgtgg tgccgttggt
     6841 agatgtgatg ttggtgttgc tggtgatttt tattatcaca gctcccttac tcacgcattc
     6901 cgttaaaatt gatttaccaa aagcttcgag taatcccaat attaccaagc cagaccatgt
     6961 ggaactggcg attcgtgaag atggcagttt atattggaat ggcgaattgg tgcagaaaga
     7021 atccctagca gcacgttttg agcttgaagc gaaaaaagag ccgcaaccag agctgcatat
     7081 taaagccgat ggcacatcga aatatgaaaa aatcgcgcag gtgatgtcga ttgcggcaaa
     7141 aaccggcctg acaaaaattg gctttgtgac tgacccatca ggcgtgcaat aagtaatatt
     7201 tcaacgtcac aaaagaagcc gtcatttcac gttaattgaa atggcggttt tttttatcta
     7261 cgtttcatgc ggaatatcag catctaaaaa ataactaatt cttttacgag ggtttaaata
     7321 atccagcgca tctttgggta gttgatttgg tcttaagcat tggctgatgc tgatattggg
     7381 ctcatcacgt aaatcaatga tgttcggctc tagacggcta gctagcgggt cgtgcagcat
     7441 gacgtatggc cgcaatggtt tgttggggtt gactgacacc acaatggcat atacacctgt
     7501 agaaagttgc accgtactgc cgggcggata tatgccgacc gttttaatga gatgtttcag
     7561 taagttactg tcaaacttgt tacgttgatg cgtatacatg agcgccagtg cttcgtaggg
     7621 agttttggca acggtgtggt cgttagggtt gcataaatta tcataccaat tcaccaaagc
     7681 gataatgttt gccagcggat cagtgagttc aggtttaatt tgtttggggt agccggtgcc
     7741 gtctgcatgc acatggtgct gtaaaataat tttaccaacg cgcgcaggta agccaacttc
     7801 tttcgccatg cgggcgccaa tttcgctatg ttgctcaaaa tgtaattgct cttgcttggt
     7861 aagcggtgct tgttttgata aaacttttcc tgaaatttca attttgccaa tatcatgaaa
     7921 aattgccgcc atgcctaata gccgcgcatc ttcttttgac atttcaatcg attttgccat
     7981 cataagcgac agcaccgtga cgttcatcgg gtgtagataa tgcgcgtcac tcgagcgatt
     8041 gccgttgagc gcatgaatgg ccacatcccc ttccatcagc gccgtttcaa ccatatcatc
     8101 cacggttagt gcggcttgtt caatcgaggt ctgcggtgtc gctagaatgt tgttggttgc
     8161 tttacgcact acatcacagg cgactagaaa ttttttctca ctttcatcca tggcatgatg
     8221 cagttgttta agcctgactt gttgaggcac tggttggagc aatggtgtgt cagcgggaac
     8281 tggaggcgca acaacttcct gattcgcaat gctttcatct tcaatagaca aatctgctat
     8341 tggtaacggt tcacagtcgc tccgtttagg gtcgtaacgt agttttttta aaccaatttt
     8401 ttggatgctg gcgatttgtg tttcatcctt cactttaaag ttgctaaagg tgaaaggatg
     8461 gtccatccag cctaaatcca gatgcacata gaggccaacg cgcagttggg aaacgtcaat
     8521 tagattatta ttgttcatgg gtattacctt ttgcatggta tttttttgtg ctgattgcca
     8581 ataacttggt aaattcagcg gctgtcacag gtttgctata taaatatcct tgtgcttcca
     8641 agcagcccat atttttcaaa aaggtggctt gtgcaagggt ttcaacccct tcagcaatca
     8701 cggtaaatcc aaggctgcaa gccattgcgt taatggtgtt ggtaatggcg gcgtcatcta
     8761 aatcatctgg caaaccgcgc acaaatgact ggtcaatctt aattttatcg agcggcaaac
     8821 gcttgaggtt agagagtgat gaatagcccg tgccaaagtc atcaatcgcc acgcgcacgc
     8881 cgagttgttt gatgttgttg aaggtgtcga tcacaaattc ggtattttgc atcaccgtgc
     8941 tttcggtaat ttcaagttcg agaatgttcg ggttgcaatt ggtttcaatc agaatgccgt
     9001 aaaccgtgtc ataaaaatta ctgcgcataa tctgcacgcc agaaacattc actgaaatcc
     9061 actgcatggt atagccatcg ttaatccact gaatggcttg caatgcagcc tgacgcagta
     9121 cccactcacc gatttgtaca atgaggccag tttcttccgc aatcggaata aatcttgcgg
     9181 gggatactaa acccaaatca ggatgacgcc agcgaattag cgcctcggcg cctataatgt
     9241 cgccggtcag caatgaaatt tgtggttggt aatacatttc aaattggtta cgctcaagcg
     9301 catggcgcaa ctggctttca atcgtaaagc gttcaactgc gtttttactg aggtcttccg
     9361 agtaaaagca gtggttattt ttgccacgat tttttacctg atacatggca atgtcagcag
     9421 ctttaatcag gctgtcaaca tctgaaccat ctttgggaaa gatggaaatg cctacgctcg
     9481 cgccaataaa aatttcttta ccgtcaataa aaaactctac ttgcagggcg tagataattt
     9541 tttgtgcaat gagagcagca tcctgcggat gcttaatcat gtccatcatc accaaaaatt
     9601 catcgccacc cagccgtgcc acaatgtcgc tatcgcgcat ggcatgatga attcgcttgg
     9661 acacttcata caataattta tcgcctactt ggtgcccaag gctatcgtta attgctttaa
     9721 agcggtctaa atcaacaaaa agcagcgcaa tttgatggtg ttctcgttca gcgcgtttaa
     9781 tggcatgttc gagtagttcg ttaaataacc tgcggtttgg caagcccgtc agcgggtcgt
     9841 gattcacgag ttcgtgtaat ctgttttgcg agtgtttggc ctcggtaatg tcggcaaaca
     9901 cccccacgta gtaaatcgtt tcatcttgtt gatttttaac ggcattcacc gtcagccgtt
     9961 cttgataggc ctcaccattt ttacggtgat ttaacaactc agaacgccat tggccatcaa
    10021 gttgcaggtg tcgccaagcc tctcgctctg tgacttgtaa cgcttttggc acggtgccaa
    10081 acacttcttg ctgattaaac ccggtaatag cagtaaaact tttgttaata cttaaaatat
    10141 tggcgtttgc atccatcacc atcatgcctt cgctggcatt ttcaaaggcg atttttgcta
    10201 agtgatatgc gctgttgcta aaagcggttg cgctttgcag tccatcttgc gatgatgctg
    10261 cttgtgtttg ggacgtgccg gtgttgctgt gataatttat atcgacccag acccaatgtt
    10321 tttccagcgc gttttgcacc gcattggata attgtgacgt gcgtaagcgg tcgccagtaa
    10381 aaggctggcc aaggttctcg aatgaatcgt tttctgaaac caaatgatag ggtttaaagt
    10441 cttgtaaatt tttcactttt tttgaacgtt tcgttatcaa atgttatcca taaacaggct
    10501 gatgacacat caacataaag cacggtataa gtggatatcg gacgcgagag agaaaacttg
    10561 agaagtttct gaataaattg aattttatga aaacaaacgc tacaaaaaaa cgatggcaaa
    10621 ttgctgtggc tttaaccatg atttatggcg caatatttgg cataaattcg ctgcaggcta
    10681 aagaaaatgg ggtggttcaa gaggctaatg tcaaacagcc accatgccca attgcgcaag
    10741 cacaagacca cagacagcaa cccattgcgt ttaaacagca catgtttgat gatggcgcac
    10801 aagatttggc ggtgattgcc gtggatgaac aacaaggacg catcacaaaa cgcatcacct
    10861 ataatcagca tcaagaagcc ggctgtcatt tttctgcggt cgcgatagcc cgaggaggcg
    10921 actggggctg gttgatggtt tgggccgatg cagaaaaaat ctattacaca cgtttagatg
    10981 gtgaagcatt ggtttttgta ccgccaaaaa aattaccgat ttcgcaggtg acgctcattg
    11041 agtttttact tgataccgcg caacccacta tgcgcgtgca aacaatgcag ggtaaaacgc
    11101 aattattgac atcggatgat gaaggtcgaa attggcagct tttgccagac caataacgta
    11161 agtatttagc aagatttttt attgcttact tcgcttgaaa aactactaga aaaaataaat
    11221 gccctgcgag cctttattta taaggcttcc agaggcatca aaaaggttgg tgttaaaaat
    11281 aggccttttt ttataaaaaa tacgtattaa taatacgcgc tgcaagtttt tagaaattat
    11341 tttttttggc gcttactgcc atccatcttc gggtttgccc ttattgaaaa aaccacaatt
    11401 tgctccatag tagcttcata tcatttatga ggagcaacag aatgcgattt gataaactca
    11461 ctaccaaatt tcaacaggcc attaacgatg cgcaaagctt ggctttgggc gcagataaca
    11521 ctgcgattga gccgcagcat ttattgctgg cgttgctgaa tcaagaggac ggcggcacgg
    11581 cgtcactgct ttcgcgcgcg ggtgtgcagt taaatccgct gaaaaccagt ttgaatactg
    11641 ccatcaacaa tttgccaaaa tcacctgatg caagcggtga agtggcgatt tcgcgtgatt
    11701 tgaataactt gttgaatgta actgataaat tcgcgcaaaa acgtggtgat tcctacattg
    11761 cgagtgaaat gtttttattg gcactggcgg atgataaagg tgaagtaggt cgattgctta
    11821 aacaaaatgg cctgacgaag tcggcgttag aagctgcgat tacggcagtc cgtggcaatg
    11881 aaaatgtgaa tagccaagaa gccgaaggcc agcgcgaatc tttgcaaaaa tataccatcg
    11941 acctcactga gcgcgctaaa ctgggcaaac ttgaccctgt catcgggcgt gatgatgaaa
    12001 ttcgccgtgc gattcaaatt ttagggcggc gcaccaaaaa caacccagtg ctgattggtg
    12061 agccaggcgt gggtaaaacg gccattgttg aaggcttggc gcagcgtatt gtgaatggcg
    12121 aagtacccga tacgctgaaa ggcaaaaaag tattgtcact tgatatggcg gctttgttag
    12181 cgggtgcaaa gtaccgaggc gagtttgaag agcgtttaaa atctgtgctc aaggaattgg
    12241 cgcaggacga aggccaaacc atcgtattta ttgatgaaat tcacaccatg gtgggtgccg
    12301 gcaaagccga gggcgcaatg gatgcgggca atatgctaaa acctgctcta gcacgcggtg
    12361 aattgcattg cgtgggcgca acaaccttag atgaatatcg taaatatatt gaaaaagatg
    12421 ccgcccttga acgccgcttt caaaaagtga tggtggatga gccgaccgta gaagcgacaa
    12481 ttgcgatttt gcgcgggcta caagaaaaat acgaattaca tcatggtgta gaaatcaccg
    12541 accctgcgat tgttgcggca gccgagctat ctcatcgcta cattacggac cgctttttac
    12601 ccgataaagc cattgattta attgatgaag cggcaagtcg tatcaagatg gaaattgatt
    12661 ccaaacctga ggtgatggat aaactcgacc gccgcttgat tcaattaaaa attgagcgtg
    12721 aagccgtgcg ccgcgagaaa gacgaagcca gccataaacg ttttgagttg attgaagaag
    12781 aaatctcgaa aattgaaaaa gagtatgccg atttagaaga aatctggaaa gccgaaaaag
    12841 cgcaagtgca gggttctgcg cacataaaag aggccatcga aaaagtaaaa tttgagatgg
    12901 aagaagccac ccgtaaaggc gagtggcaga aagtgtctga actgcaatat ggcaaattgc
    12961 cgcagttaga ggcgcagtta aaacaagcat ccagtgctga agcggaaagc gggcaagtaa
    13021 aacacaaaat gctgcgcacc gaagtgggcg ccgatgagat tgccgaggtt gtgagccgcg
    13081 ccacagggat tcctgtgagc aaaatgttaa cgggcgagcg cgaaaagtta ttgaccatgg
    13141 aagcgaaact gcacgagcgc gtggtagggc aagacgaggc ggtgcgtttg gtgagtgatg
    13201 cgattcgccg ttcacgctca ggcttaagcg accccaatcg tccctatggc agctttttgt
    13261 ttttaggccc aacgggtgtg ggtaaaactg agttgtgtaa atcgctggcc aatttcttgt
    13321 ttgattcaga agaccatttg attcgcatcg acatgagcga atttatggaa aaacattctg
    13381 tggcacggat gataggcgcg ccacccggct atgtgggcta tgaagaaggc ggtacattga
    13441 cggaagccgt gcgccgcaag ccttacagcg tgattttgct tgatgaagtt gaaaaagcgc
    13501 accctgatgt gtttaatgtg ctgttgcagg tgctggatga tggccgccta accgatggcc
    13561 aaggccgcac ggtggatttc aaaaatacag tgattatcat gacttctaac ttaggtagtc
    13621 agatgattca aagcatgtca gatcaagatt accaagtggt gaaactggcg gtgatgggtg
    13681 aggtgaaaac gcatttcagg ccagagtttg tcaaccggat tgatgaagtg gtcgtttttc
    13741 acgcattggg tgaggcaaat atcaagtcaa ttgcaagcat tcaattgcaa tacttggcca
    13801 aacgtttggc tgcaatggac atgcaactga aggtgactga tgcggcactg gctgaaatcg
    13861 ccagcgcagg gtttgacccc gtgtatggtg cacgtccatt aaaacgggcc attcagagtg
    13921 agattgaaaa cccattggca cgtgaaattt tagccgggaa ttttatggcg aaagatacag
    13981 tccaagtaga ttataaagct ggaagaatgc agtttagtaa agtttgatgg tttgtaggcg
    14041 cgcaatacca caggcataag taattctaat catgcacagc atgaaagtat tactaatcaa
    14101 ttgcgcgcct acaaaataag cccatgagag gaatcagcga tgaataagca aagtttaatg
    14161 ttgagtacag tattgctgac cgccttattg gctggctgcg ccagcactgc gccgcgtgag
    14221 gtggctgttg ataggcttac gcctgaagaa gtggcacaaa ttacacctgc ggctacgcca
    14281 acattgagtt tagacgagat tgttagttta acgcagcaaa aagtctcgtc tgacgagatt
    14341 attgctaaaa ttaaggcgag caactcgcaa tatgatttga cagccagcca aattgtagac
    14401 ttaagcaagc aaggcgtgga ttcaaaagtg ctggattaca ttcagactga acgtgaaaag
    14461 gcacgcctaa atagccttgc agatgaaatc aataaacgcg aaaaagccaa taaagaagcg
    14521 caagaaaagc tcaaacaaca agcttggcgc aatcgctacg acccctttta tgacccgttt
    14581 taccaccctt tttatggtta cggcccttat tggggaagtc acttttactt gaacaaccat
    14641 ttacatcggc gtagaagata acgcaaaaaa actcgcgcta taatggcgta ttgtgtttga
    14701 gggtttttac atgcgccaat tttcagaaca ggtcaccatt aaaacagcag gccgtaaact
    14761 ttacgacatt actcctgatg ttttacaact taccaaaaaa gctgctattc agacgggttt
    14821 gctcacactc tatattcagc atacctctgc aagcttgctg attaacgaaa attacgatgc
    14881 cgacgtgctg gtggatatgg aggcattttt taatcgactg gtgcctgatg gcgataagct
    14941 ctttattcat accgttgaag gtcctgacga catgcctgca cacgtgcgta gtgcgttgac
    15001 gcaaaccagt ttatctattc caattatgca aggcaggcca gcactcggcc agtggcaggg
    15061 tatttttttg tatgaacacc ggcatgttgc ggcgagtcgg cgcgttattt tgcatgcaat
    15121 aggagattaa ttttgggcaa gcgtggccgg aaattaagcc ctgcggaatg gcagatttat
    15181 cagccttatt tttcacccga agtattgcat tacgcgcgca ttgttgatgg gcatacgcca
    15241 ttttggttac ggccaagcat gtgtgcagtg gtgttacagc aaaaaatcta ttttcgagct
    15301 ggttattatc agcccagcac attgcagggt atagcattgt tagggcatga attaacacat
    15361 gtagagcagt ttttacaagg catgacaatc tggcattatt tgtgggaatg ccgacatggc
    15421 tatgtaaaaa atcgttatga agtggcagct tatgcgaaag gtgctatgat tagttacgat
    15481 gtcgccaaac gaggcattat ttagggtttt tactaaaata aaatctacaa attttttaag
    15541 gagaagaaca aatgggattg ttttcattta ttaaagaagc aggcgaaaaa ctatttggta
    15601 ttggcgaagc aaaagcggca gaagaagtcg caaaaaaaga cccaacaccg gtgaatgttg
    15661 aagctgcaaa taaagctgct gcaaaagcga ttgctgatta cgtggcaaaa atgggtttaa
    15721 cggccgaggg cttgaccatt ggctttgatg gtgcatccgc cacggtgatt gtacaaggca
    15781 tggcagacac caaagaaacc aaagaaaaga ttttactctg ttgcggtaat gtaaaaggcg
    15841 tggaacatgt gcaagaccaa ttgggtgtga ttgaacctgc agatgagtct gtgttctaca
    15901 ccgtcgtgag tggcgatacg ctatcaaaaa ttgctaaagc gcagtatggc aatgccaatg
    15961 actatatgaa gatatttgaa gcgaataaac ccatgttaag ccacccagat aaaatttatc
    16021 ctggccagtc attgcgtatt cctaactaaa cggtacgact ttgcattaag tcattcattt
    16081 taaatgactt taagtttact ttcctgagaa atgtcgctag aatgcaactt cgagtttagt
    16141 cagataaaaa atccttgtta cttgtcaata gttcaaggat ttttttatgt taatcatcaa
    16201 gttgaaagga attaaaatgt ctacattatt acgtgcttta ttagcactaa gcgttaccag
    16261 tttgttgctc gtcggttgtg gtgaaaaaga agctgcacca gtggaggcta caccagccgt
    16321 tgaagcgcca gcgccagttg aagcggcgcc tgttgaagtt gcacctgcac cagcccctgc
    16381 tgcaaaagag ccaggtggct atgagccaac acctgaagag cgcgtgcctg cattaccctt
    16441 gatgattcag cgcaaccggt
//
LOCUS       DAOQSI010000027        15992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_2734_length_15992_cov_5.488047, whole genome shotgun sequence.
ACCESSION   DAOQSI010000027 DAOQSI010000000
VERSION     DAOQSI010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 15992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15992
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2734_length_15992_cov_5.488047"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1353
                     /gene="glmU"
                     /locus_tag="PKL42_03180"
     CDS             <1..1353
                     /gene="glmU"
                     /locus_tag="PKL42_03180"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HNU66106.1"
                     /translation="VILAAGKGTRMHSNLPKVLHEIGAKPILEHVIDCAKALNPDKVI
                     VVYGFGGEAVKEQFSKENITWVNQAEQLGTGHAVQQAVPHLEDRANTLILLGDVPLVD
                     VEACRKLIAHSDSKLVIQSFNKTDPAGYGRIVRSADGQVNAIVEHKDATAAQRDISEV
                     NTGIMAMPNKYLKKWLLQLTNNNAQGEYYLTDIVSLAVAEGVQVSAEITEDEWSVTGI
                     NSKTDLVQIERVYQSRIAQKLLQQGVTLKDSSRLDVRGSLACGRDVEIDVNCVFEGNV
                     TLGDNVKIAANCLIKNAVIAAGTKIAAFTHIDDTAIGENSRIGPFARLRPGTILAADT
                     HIGNFVELKNAQVDIGSKVNHLSYIGDATIGKQVNVGAGTITCNYDGANKFRTVIEDG
                     AFIGSDSQLVAPVTIGKNATIAAGSTITRDAPADSLTLCRAREQKTIVGWQRPTKIKK
                     "
     gene            1371..3203
                     /gene="glmS"
                     /locus_tag="PKL42_03185"
     CDS             1371..3203
                     /gene="glmS"
                     /locus_tag="PKL42_03185"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNU66107.1"
                     /translation="MCGIVGAVANRNVVSTLIEGLSRLEYRGYDSAGVAVLSSGSIER
                     VRAVGRVANMTEKANAVHLSGEVGIGHTRWATHGGVTESNAHPHVSKGEIAVVHNGII
                     ENHDEQRTRLKALGYEFTSQTDTEVIAHLIHYYHKQGLVLLAATKKAVAELTGAFAIS
                     VISTKEPKHMVTARLGCPLLIGLGEGENFIASDVSAVLSVTRKVIYLEDGDVADIRRD
                     GVTIFDKHDAEVARKIHLSDVSLASLELGPYAHFMQKEIHEQPRALTDTIEALIDDNN
                     FSPALFGDQGGEVLKQADSILILAAGTSYYAALTAKYWLESIARIPTNVEIASEYRYR
                     DSVPNPRQLIVTISQSGETLDTMEALKHAKSLGQNLTLAICNVQESAIPRASALVFYT
                     RAGAEIGVASTKAFTTQLVALFTLAATLAKLRGLLSVALEQEFLTALRQLAGSVQHAL
                     NLEPQIREWAKRFADKEHALFLGRGIHYPIALEGALKLKEISYIHAEAYPAGELKHGP
                     LALVDNNMPVVVIAPNDALLEKVKSNMQEVNARGGELFVFADADSHFTESEGVHVIRT
                     PRHVGVLSPILHTIPVQMLAYHAALLKGTDVDKPRNLAKSVTVE"
     gene            complement(3361..4599)
                     /locus_tag="PKL42_03190"
     CDS             complement(3361..4599)
                     /locus_tag="PKL42_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HNU66108.1"
                     /translation="MTIENALWLNLAVALGIGLLIGAERERSKGNGPERSAAGIRTFT
                     ITTLLGAVSIVFNVWLLIASIFCVMIFAATAYYSKRTEDPGLTTEITLIFSVILGGLA
                     MSEPILAAALAVSIAILLSAKEPMHGFVLATVTKDELNDFLILAAATLIILPLVPNAF
                     VGPFEAINPRNLWLIVILIMTISALGHLALRWLGGRVGLPLVGLFSGFISSIATISAM
                     GARVKESPHLLSATVAGAVLSSLATILQIAMLLLAIHPPSLIALQWPLIFGGLSIAIY
                     GLIATVNCYHDPHVDAHKQTKTFSVKTALLFAAIIAIILIASAALNAWFGQAGLVVAS
                     GVAGLADVHAAVISITSFVASGKISIQSAVIPILVAFSVNTLVKAVIAQVTGGTQFAR
                     LVVPALAIQVAAVWLGWWLM"
     gene            complement(4647..5177)
                     /locus_tag="PKL42_03195"
     CDS             complement(4647..5177)
                     /locus_tag="PKL42_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HNU66109.1"
                     /translation="MAIKSTIYKIDLQIADMDRNYYALHNLTLARHPSETDERLMVRL
                     IAFTLYANEALTFGKGLSDDEEPDLWQKDLTGAIQLWIDVGLPDEREIRKACGKSAQV
                     VVVLYGGRVADMWWAQNSKALLKLNNLTVINLPDTEALTNIASRGLNISCTIQDGQML
                     VGHETGSVDITPITLK"
     gene            5331..6995
                     /gene="ettA"
                     /locus_tag="PKL42_03200"
     CDS             5331..6995
                     /gene="ettA"
                     /locus_tag="PKL42_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HNU66110.1"
                     /translation="MAQYVMSMLRVSKIVPPKRTIIKDISLSFFPGAKIGLLGLNGSG
                     KSTVLRIMAGVDKEYEGEVQWQPGISIGYLPQEPQLDANKTVREEVESGMGEVMQAQA
                     NLDAVYAAYAEPDADFEKLAEEQAKWENIIAASGADVGHQLEIAADALRLPPWDAKIG
                     PLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAVTHDR
                     YFLDNAAEWILELDRGHGIPWKGNYSSWLDQKQARLALEESQESSRRKALNTELEWVR
                     QNPKARQAKSKARIARYEELASAEYQKRNETQEIFIPAGERLGDQVIEFNNVSKAFKD
                     RLLIDNLSLSIPAGAIVGIIGPNGAGKSTIFKMITGLEQPDSGEVKIGQTVKLAYVDQ
                     SRDDLSNEKTVFDEISGGSDILTVGRYETPSRAYIGRFNFKGGDQQKVVGQLSGGERG
                     RLHLAKTLISGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVLVISHDRWFLDRIAT
                     HILAAEGDSQWTFFNGNYQEYEADKRKRLGEEGAKPKRIRYKPITR"
     gene            complement(7059..7262)
                     /locus_tag="PKL42_03205"
     CDS             complement(7059..7262)
                     /locus_tag="PKL42_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNU66111.1"
                     /translation="MSSAKNDLSDEIMCPCSGTTRGKIQKLFEDGLDIDAISRRTGAI
                     SGCAGCEWDIAQFVKELAGLQGK"
     gene            complement(7305..8093)
                     /locus_tag="PKL42_03210"
     CDS             complement(7305..8093)
                     /locus_tag="PKL42_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNU66112.1"
                     /translation="MKIFITGASSGIGEALAHAYAKTSAERPIFIGLAARRQDVLEKL
                     ACLLQTQPNVECVIYLLDVKDSLALDFAAKDFIQRFGAPDLVIASAGVSRGTLTEYQE
                     DNAAFQAMMQINVLGMVHTFQPFIAAMKSAGHGHLVGIASVAGIRGLPGAGAYSASKA
                     AVINYLESLRAEMLHDNISVTTIVPGYIRTPLTDVNQFRMPFLMDADDFAKKFIHHVA
                     SKRRVCVIPWQMGIVVKLMRLLPPALWDCLVKNAPQKSRVIEKL"
     gene            complement(8240..8893)
                     /locus_tag="PKL42_03215"
     CDS             complement(8240..8893)
                     /locus_tag="PKL42_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HNU66113.1"
                     /translation="MLKKLLASFLLISSFNLMAADPQIGQDFEKTAQVITTENPVKIE
                     VNEIFWYGCIHCYHMDPVLEAWAKKLPADVIFKRTPGLPNPSWAPMAKAFYTLEELKL
                     LDKLHTPLFDAVHKQKALNPTDEKAIIDWITKQSGLDKIKIEEHFKSFSMNNKLNRAA
                     KMFKDSGATGVPSLVIDGQYITSSTMVGGNAQALRVTDYIVGNIRADKAKAATPAKK"
     gene            complement(8990..9610)
                     /locus_tag="PKL42_03220"
     CDS             complement(8990..9610)
                     /locus_tag="PKL42_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNU66114.1"
                     /translation="MSKDYKQSAERQTRSKKGSPFLTGLLIGILLGIAASLAVVVYLK
                     RDDNPFAIQTNKQTSKSLSDKIAEDAKSQEKNATASKDISPEASANNDDTSYDFYTIL
                     PSDENKISKEVESTLKANNEPATNKTYYLQVGSFHTEEEADNLKAKLALQGFEAVVQT
                     ADIPEKGVWHRVRVGPLNDLDQITKTKTDLLNNGFNADLIKINSES"
     gene            complement(9625..11280)
                     /gene="argS"
                     /locus_tag="PKL42_03225"
     CDS             complement(9625..11280)
                     /gene="argS"
                     /locus_tag="PKL42_03225"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNU66115.1"
                     /translation="MKTHLTQLLANAAKTIAPELQVAILLERPKSADHGDFATNLALV
                     LAKPLKQNPRAIATQLIAAMPNSDYIAKTEIAGAGFINFFLNSHSQQKVVKDILQAGH
                     QYGRNQNGQGQKVQVEFVSANPTGPLHVGHGRGAAVGDCLARLLDANGWDVTREFYYN
                     DAGAQIDNLTISVLARCKGISTEHESFPENGYRGEYINDIAAAYLTKHTVIADDIEVT
                     ASGDINNLDSIRAFSVAYLRHEQDLDLKAFQIKFDVFSLESALYTTGKVKDTVQALIN
                     SGHTYEANDALWLKTTDFGDDKDRVMRKTDGSYTYFVPDIAYHTDKWNRGFVRVINEQ
                     GADHHSTITRVRAGLQALNIGIPNGWPDYVLHQMVTVMRGGEEVKLSKRAGSYVTLRD
                     LIEEVGCDATRYFLAARRADSQLVFDIDLARAQTNDNPVFYIQYAHARICSVLTQWNG
                     DKSLLLSADLSPLDNPHETALMQRLSAYSETVENAANELAPHVIANYLKDLASDLHSY
                     YNEYKFLIEDETVKMARLSLIYATQQVLKNGLDLLGVTAKERM"
     gene            11376..13595
                     /locus_tag="PKL42_03230"
     CDS             11376..13595
                     /locus_tag="PKL42_03230"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNU66116.1"
                     /translation="MPKSIVKVALDVPLNRLFDYLSDNSEIRIGQRVLVPFGRRNQIG
                     IVLSQSDSSDYPIEKLKSVKQVFIDETPLDAELLKLIRFSADYYQYPLGQALLSALPV
                     RLRQVAPAVARKQFIYSLTDLGRSQTVAQLPKRQLVTHRVFSALLASNTVSEAELDTI
                     SSSARKAAKQLVANGWASAKQVHLLQRVAEIPTAQVPELNDEQQSAVAEVIKDSHHFK
                     SWLLHGITGSGKTEVYIRLMQHVLMQNEAQVLVLVPEINLTPQLEARFRSRFSGIPLV
                     SLHSHLSESERLHHWQLAQSGVARIIIGTRLSVFTPLPHLKLIIIDEEHDGSYKQQDS
                     MRYHARDVALVRAKWLNIPVVLGSATPSLESWHNATVTAAGLKANKYHLLSLNQRAVT
                     AAQLPIIHCIDTTKVNLQHGFTPQLIEALKLRISRGEQSLLFINRRGFSPVLLCSACH
                     WIAPCTRCSSRLVVHLGQRKLRCHHCGYEQKIPNQCPSCGNADLHPTGHGTQRLEQTL
                     AQLLPTARIARVDKDSISKKNALVEILDKVHRQEIDILVGTQMLAKGHDFPNLTLVGV
                     IDADSALHSPDFRASERLFAQLMQVAGRAGRADKTGQVIIQTQFPGHVLFNALRRQDY
                     ASYANALLHERQQMQFPPYIFMALLRAEANDFNLVHQFLSYAFKVARDLNTKVVVYDP
                     IRPQMERLKGMERGYVLMQAGNRGVLQKLLNRLVAQLRGHTASAKVRWAIDVDPLEF"
     gene            13714..14727
                     /locus_tag="PKL42_03235"
     CDS             13714..14727
                     /locus_tag="PKL42_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family peroxidase"
                     /protein_id="HNU66117.1"
                     /translation="MTTFSRHLYTPILFALLGLSNACLAQADDKQPDSAKSVVEQIVD
                     VQTKIASGPHKGFRANHAKGIIATGIFTPSAEAAGLTSAVHLQNTASEVIVRFSNATG
                     IPTIADTSPKANPRGIAIRFKLADEAYTDIVSLSVNRFPVSTPEAFLALLQANAATKA
                     DSPKPTPIEQFLAENPSAQAFVKIPKPAPASYATQSYFGINAFKFTNAKGESRYGRYL
                     ITPLAGNIVLDEAKVASASPDYLSEELAARLNQGEASFRISVQVANDGDSINDPTVVW
                     PEDRAQVALGTLTLKAIPADSKTIEQKITFNPLILPDGIAPSEDKILLERPAAYAVSA
                     SRR"
     gene            14849..14922
                     /locus_tag="PKL42_03240"
     tRNA            14849..14922
                     /locus_tag="PKL42_03240"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14881..14883,aa:Gly,seq:ccc)
     gene            15088..>15992
                     /locus_tag="PKL42_03245"
     CDS             15088..>15992
                     /locus_tag="PKL42_03245"
                     /inference="COORDINATES: protein motif:HMM:NF024750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HNU66118.1"
                     /translation="MKKLTTSALDDLTIKAAKAKEKAYTLRDGNGLFLLVHPNGSKYF
                     QLRTTLHGKAKLIQLGTYPILSLSEARDLGREKLKKVKVEQIDPVIEAKLLKHQKAKD
                     ASNIFEDVANEWLAIKQRTLAPTSHTKIKQTFNANVFGVLGKYPIKDIDNQMVRKCLM
                     IMQKRGALELMEQTRAWIKQVFEFALSDNLIAENPIPSIDVRLEKHNPEKYPRLQSRQ
                     DAGTLLRNITKYKGSFEVITCVYLQMHFAQRPSELRCAKWSEFDLDNAVWTIPLPRSK
                     TRKHMTKPHTVMLSRQAGKMCWRLKS"
BASE COUNT         4564 a         3425 c         3665 g         4338 t
ORIGIN      
        1 gtgatacttg ctgccggtaa aggcacgcgc atgcattcaa atcttccaaa agtactgcat
       61 gaaattggag caaagccaat attagaacat gtgatagatt gtgcaaaagc gctaaatcca
      121 gacaaagtga tcgtggtata tgggtttggt ggtgaagctg tcaaagagca gttttctaaa
      181 gagaatatta cctgggtgaa ccaagcagaa cagctaggaa ctggccatgc ggtgcaacaa
      241 gcagttccac acttggaaga ccgtgctaat acgcttattt tgttaggtga cgtgccactg
      301 gtagatgttg aggcgtgtag aaaattaatt gctcattcgg atagcaagtt ggtgattcaa
      361 tcgttcaata aaactgaccc tgcaggctac ggtagaatag tccgctcagc cgatggtcag
      421 gtcaatgcaa ttgtagaaca taaagatgca acagcagcac agcgcgacat tagtgaagtg
      481 aatacaggta ttatggcgat gccaaataag tatttaaaaa aatggttatt acaacttaca
      541 aataataacg cacaaggtga atactattta acggatatcg tttcattggc tgtagcagaa
      601 ggtgtgcaag tgagtgctga aatcaccgaa gacgaatggt cggttactgg cattaattct
      661 aaaactgatt tggtgcaaat tgagcgcgtg tatcaaagta ggatcgccca aaagctgcta
      721 caacagggcg tcacgcttaa agactcatca agattagacg tacgtggcag tttagcctgt
      781 ggccgtgatg tggaaattga tgtgaactgc gtatttgaag gcaacgtgac gttaggcgac
      841 aacgtaaaaa tcgctgccaa ttgtctcatt aaaaatgccg tgattgctgc tggtaccaag
      901 attgctgctt ttacacacat tgatgatact gcaattggtg aaaatagccg aattgggcca
      961 tttgcacgat tacgcccagg cactatactt gctgctgaca cgcatattgg caattttgtc
     1021 gagttgaaaa atgcgcaagt agatattggt agtaaagtaa atcatttaag ttatattggt
     1081 gacgcaacta taggcaaaca agtaaatgta ggtgccggca cgattacttg taattatgat
     1141 ggcgccaata aatttagaac ggtgattgaa gatggcgcct ttattggctc agacagccaa
     1201 ttagttgcac cagtcaccat cggcaaaaat gcaactattg cggctggctc taccatcacg
     1261 agagatgcac cagcagactc gttaacgtta tgccgtgcaa gagaacaaaa aacgattgtg
     1321 ggttggcaac gacctactaa aattaaaaag taagaaagca tcaattaact atgtgtggaa
     1381 tagtcggcgc agttgccaat agaaatgttg tatcaacctt aatcgaaggt ttaagtcgct
     1441 tagagtatcg cggttatgac tcagcaggcg ttgctgtgtt aagtagcggt agcattgaac
     1501 gcgtgcgtgc agtgggccgt gtagcgaaca tgactgaaaa agcaaatgct gtgcatttaa
     1561 gtggggaagt cggtattggc cacacgcgct gggccacgca cggtggcgtt acagagagta
     1621 atgcacatcc acatgtatca aaaggtgaaa ttgcggttgt acataatggc attattgaaa
     1681 accacgatga acaacgcacg cgtttaaaag cgctaggtta cgagtttacc tcgcaaacag
     1741 acaccgaagt gattgctcac ttgattcatt attatcacaa acaagggcta gttttactag
     1801 ccgcaactaa aaaagcggtg gcagaactca ctggtgcatt tgcaattagc gtaatttcaa
     1861 ccaaagagcc taaacacatg gtaacagcac gtttaggttg cccgcttttg attggtttgg
     1921 gtgaaggtga aaactttatt gcctcagatg tctctgcagt gctgtcggtc acgcgtaaag
     1981 tcatctacct tgaagatggc gatgtagcgg acattcggcg tgatggcgtt acaatattcg
     2041 ataagcatga tgcagaagtc gcgcgcaaaa ttcatttaag tgatgtgtca cttgccagcc
     2101 ttgagttggg cccttacgcg cattttatgc aaaaagaaat tcacgagcaa ccacgcgcgc
     2161 tgactgacac catagaagca ttgattgatg ataataattt ctcccccgcc ctatttggtg
     2221 atcaaggcgg tgaagtatta aagcaagcgg acagcatttt aattttggcc gcaggcacca
     2281 gttattacgc agcactcaca gcaaaatatt ggctggagag cattgccaga attccaacca
     2341 atgttgaaat cgcctcagag tatcgttata gagactctgt gccaaaccca cgtcaattga
     2401 ttgtgacgat ttcacagtcg ggtgaaacac tagatacgat ggaagcactg aaacatgcaa
     2461 aatcgttagg gcaaaactta acactggcca tttgcaatgt gcaagaaagc gctattccgc
     2521 gtgcttcagc acttgtgttc tacacgcgcg caggtgcaga aatcggcgtg gcttctacca
     2581 aagcattcac tacacaatta gtcgctctgt tcaccttagc ggcaacgctc gcaaaactgc
     2641 gcggcttact tagtgtggct ttggagcaag aatttttgac agcattacgc caactggcgg
     2701 gcagcgtaca acatgcgctc aatctagaac cgcaaatacg tgaatgggca aaacgctttg
     2761 cggacaaaga acatgcgtta tttttaggcc gcggcattca ttaccccatt gccttggagg
     2821 gcgcgcttaa actgaaagaa atttcttata ttcatgctga agcctaccct gccggtgagt
     2881 taaaacatgg cccgttggca ttagtggata ataatatgcc agtcgttgta attgcgccca
     2941 atgacgcgtt gctagaaaaa gtaaaatcaa acatgcagga agtcaacgca cgcggcggcg
     3001 agctttttgt atttgccgat gcagatagcc attttaccga gagcgaaggc gtgcatgtga
     3061 ttcgcacgcc gcggcatgtg ggcgtactct cccctatttt gcataccata cctgtgcaaa
     3121 tgctcgctta tcacgccgct ttacttaaag gcactgatgt tgataagcca agaaatttgg
     3181 caaaaagtgt gacagtagaa taggttttaa aaagcattta acctgctatt tcaaacaaca
     3241 acctttttcg atgccctgga agccttataa ataaaggctt ccagggcata aattttttct
     3301 cgtagattta agtgcgttat aagtgtaatt attagtgtgc aaaaatggcg taaaaactta
     3361 tcacattaac caccagccca gccagacggc tgccacttgt atggctagtg caggcaccac
     3421 taatcgtgca aattgagtgc cgccagttac ctgcgcgatg acggctttaa ccaaagtgtt
     3481 gacactaaat gcgacaagta ttggtatcac agcactttga atgctaattt ttcctgaggc
     3541 aacaaaagag gtaattgaaa tcactgcagc atgaacatcc gctaaacccg caacgccaga
     3601 ggcaaccact aaacccgctt ggccaaacca cgcgttaagc gcggcagaag caattaatat
     3661 gatggcgata attgcagcaa aaagcagcgc agtttttacg ctaaatgttt tggtttgttt
     3721 gtgtgcatct acgtgcgggt catgatagca attgaccgtt gcaattaagc catagatggc
     3781 aatagataag ccaccaaaaa tcagtggcca ttgtaaggct attagtgagg gtggatggat
     3841 ggcgagtagc aacatggcaa tttgtaaaat ggtcgccaag cttgataaca cggcgccggc
     3901 aactgtggcg ctgagtaaat gcggagattc tttgacgcgt gcgcccatgg cgctaatggt
     3961 tgcaatgctt gagataaatc cagaaaataa accaacgagt ggcaacccta cccgcccgcc
     4021 tagccagcgt agcgccaagt gaccaagggc gctgatggtc atgatgagaa tgacaataag
     4081 ccaaagattg cgcgggttaa tggcttcaaa tggccccaca aacgcattag ggaccaaggg
     4141 caagatgatg agggttgcag cggctaaaat gaggaagtca ttaagctcat ctttagtgac
     4201 agtggccagc acaaacccat gcatcggttc tttggctgaa aggaggatgg ctatgctcac
     4261 cgctaatgcc gcagccaaaa tgggttcgct catggctaat ccaccaagaa ttactgaaaa
     4321 tatcagggtg atttctgtcg taagacctgg gtcttcagtt cttttggaat aataggccgt
     4381 tgcagcaaaa atcatcacgc aaaagatgga agcaatcagc agccaaacat taaatacgat
     4441 gctgacagcg cctaataaag tggtgatagt aaaggttcga ataccggcag ctgaacgttc
     4501 tgggccgttc cctttactgc gctctctttc ggcgccaatg agcaggccaa tgcccaaggc
     4561 cacggccaaa ttaagccaaa gtgcgttttc aatagtcatt tgcattatgt gaacgattta
     4621 aacatgcgta acaaagttgt tatacgctat tttaatgtga ttggcgtaat gtcaacactc
     4681 cctgtttcat gaccaaccag catttggcca tcttgaatgg tgcaactgat atttaaccct
     4741 cggcttgcga tgttagtcag tgcttcggtg tcgggcaggt taattaccgt gaggttattg
     4801 agttttagca acgctttgct attttgcgcc caccacatat ctgcaacgcg tccgccgtaa
     4861 agtacaacga ccacttgcgc agattttcca caggccttac gaatttcacg ttcatctggc
     4921 agaccgacat caatccaaag ctgaattgcg cctgtgagat ctttttgcca taaatcgggc
     4981 tcttcatcgt cacttaggcc cttgccaaat gtgagcgctt catttgcata aagtgtaaaa
     5041 gcgatgaggc gcaccattaa gcgttcatct gtttcagaag ggtgtctggc taacgtgaga
     5101 ttgtgtagcg catagtaatt gcgatccata tctgcaattt gcagatcaat tttgtatata
     5161 gttgatttta ttgccataac ggtttaaatg gctttcgtaa aagacttaaa aagtgcgtac
     5221 attatacctt tatcgccaag cgggctgaca caatccagca tggtgttgat tcacaaggga
     5281 aggcgacttt ctgttaagct tgcattcttt caaatttaca aattatcatc atggctcaat
     5341 acgtaatgtc aatgctccgc gtgagcaaaa ttgttcctcc taaaagaaca atcattaaag
     5401 atatctcttt atcgtttttt ccaggcgcta aaattggttt gctcggctta aacggctctg
     5461 gtaagtctac cgtactacgt attatggctg gggtcgataa agagtatgag ggtgaagtgc
     5521 aatggcagcc cggcatttca attgggtatt taccgcaaga accgcagctg gatgccaata
     5581 aaaccgtacg tgaagaagtg gaaagtggca tgggtgaagt catgcaagcg caggcaaatt
     5641 tagatgccgt gtatgccgcc tatgccgagc cagatgcaga ctttgaaaaa ctggctgaag
     5701 aacaagccaa atgggaaaac atcattgccg ccagcggagc agacgttgga catcaattag
     5761 aaattgcggc cgatgcgcta cgtttgccac catgggacgc gaaaattggc ccattatcag
     5821 gtggtgaaaa acgtcgcgtg gctttgtgca aactgttgct atcaaagcct gatatgctgc
     5881 tgttagacga gccaaccaac catttggatg cagagtcagt ggagtggctg gaacaattcc
     5941 tagtgcgctt tcccggcact gtggtggccg tcacgcatga tcgttacttc cttgataacg
     6001 ccgctgaatg gattttagag ttagaccgtg gtcatggtat tccatggaaa ggcaattact
     6061 ctagctggct agaccaaaag caagcacggt tggctttaga ggaatcacaa gaatcatctc
     6121 gccgtaaagc gctaaacaca gaattagaat gggtgcggca aaatccaaaa gcgcggcaag
     6181 ctaaaagtaa ggcacgtatc gcccgttacg aagaacttgc cagtgccgaa tatcaaaaac
     6241 gcaatgaaac ccaggagatt tttattcctg caggtgagcg tttaggtgac caggtgatag
     6301 agtttaataa tgtcagtaaa gcgtttaaag accgtctgct gattgataat ttgagcttga
     6361 gtattccagc aggtgcgatt gtcggtatta tcggcccgaa tggcgcgggt aaatcgacga
     6421 tatttaagat gattacaggc ttagaacaac ctgatagtgg tgaagtgaag attggccaaa
     6481 cagtaaaact ggcgtatgtt gatcaaagcc gtgatgactt aagtaatgag aaaaccgtgt
     6541 ttgatgaaat ttctggtggc tcagatattc tcaccgtggg tcgttacgaa acgccctcgc
     6601 gcgcctatat tggccgcttc aactttaaag gcggcgacca gcaaaaagta gttggccaac
     6661 tttcgggcgg tgaacgtggc cgtttgcact tagcaaaaac gcttatttct ggtggcaatg
     6721 tgttgctact cgatgagcca tctaacgatt tagatgttga aactttacgc gcactagaag
     6781 atgctttgtt ggagttcgct ggttcggtat tggtgatttc acatgaccgt tggttcttag
     6841 atcgtatcgc cactcatatt ttggctgcag aaggcgattc gcaatggacg ttctttaatg
     6901 gaaactatca ggaatatgag gccgataagc gtaaacgttt aggtgaagaa ggtgctaaac
     6961 cgaaacgcat tcgatataaa ccaataacac gctagatttt tttcttggac tttttagtgg
     7021 atatgggcgg agatactttg cctatttccc tgatggtgtt atttaccttg cagcccagcg
     7081 agttctttta caaattgtgc aatatcccac tcgcagccgg cacaacctga aatagcgcct
     7141 gttcttctag aaatcgcgtc aatatccagc ccatcttcaa acagtttttg aatcttgcca
     7201 cgcgtagtgc cactgcacgg gcacataatt tcatcggata agtcgttttt tgcagaactc
     7261 attggtgaag tatttctaaa taaatagtgt taatgattag tttgttaaag tttttctata
     7321 acgcgtgatt tttgaggcgc atttttaacc agacaatccc aaagcgccgg cggtaaaagg
     7381 cgcatcagct tcactacaat acccatttgc catggaatca cacaaacgcg gcgtttagag
     7441 gctacgtgat ggataaattt cttagcaaag tcatcagcat ccataagaaa aggcatacgg
     7501 aattggttta cgtccgtcaa gggcgtgcga atataacctg gcacaatggt ggtgacgcta
     7561 atattatcat gcagcatttc tgcgcgcaga ctttctaaat agttgatgac tgctgctttg
     7621 ctggcactgt aagcacctgc gcctggtaaa ccgcgaatgc ctgcaacgct ggcaatccca
     7681 actaaatggc cgtgccctgc acttttcatg gctgcaataa aaggctgaaa tgtatgcacc
     7741 atgccaagca cgttaatttg catcattgct tgaaacgcag cgttatcttc ttgatattcg
     7801 gtgagcgttc cgcggctaac acccgcgctc gcaatcacaa gatctggtgc gccaaagcgt
     7861 tgtataaagt ctttggccgc gaaatccaat gcaaggctgt ctttgacatc aagtagatat
     7921 attacacatt caacattggg ttgcgtttgt aaaaggcaag ctagtttctc taatacatct
     7981 tggcgccgag ctgccaagcc aataaatatc ggacgttcgg cgctagtctt tgcgtaggcg
     8041 tgagctaatg cttcaccaat gccgcttgat gcgcccgtga taaatatttt catatctaga
     8101 taaggcctta taaaagtaaa aaacccgttt cgccttaaat ggtgaaaacg ggtactgaaa
     8161 agtaattggt atggtttgtt aaatgtctac caattaaaaa cttttttcaa gaaaaacgat
     8221 agccaacttg atttttcaac tacttttttg caggtgttgc cgccttcgct ttatctgcgc
     8281 gaatgttgcc gacaatatag tcagtcactc ttaaggcttg cgcatttccg cccaccattg
     8341 tgctggaggt gatgtactga ccgtcaatca ccaagcttgg cacgccagta gcaccagaat
     8401 ctttaaacat tttggcagcg cggttaagtt tgttattcat actaaaggac ttaaaatgct
     8461 cttctatttt aattttatct agcccacttt gtttggtaat ccagtcaatg attgcctttt
     8521 catcggttgg attaagcgct ttttgtttat gcaccgcgtc aaacaatggc gtgtgtaatt
     8581 tatcgagtaa ttttaattct tccaatgtat aaaacgcttt ggccattggc gcccatgatg
     8641 ggttaggcaa cccgggagtg cgcttgaata tgacatcagc aggcaatttt ttggcccatg
     8701 cttcaagcac tgggtccata tggtaacaat gaatacagcc gtaccaaaag atttcattca
     8761 cttctatttt gactgggttt tctgtagtga ttacttgtgc ggtcttttca aaatcttggc
     8821 caatctgagg gtcagccgcc attaaattga aagaagagat taacaaaaat gaagcgagta
     8881 atttttttaa cataaataat ccttaaattt ctttctagtt ttatgatgta aaactgtcaa
     8941 aacatgcaac taacgtctga catagtgttt ggctgacagg ttccggcaat tagctttcac
     9001 tattaatttt aatgaggtct gcattaaaac cattattcag taaatccgtc ttggtttttg
     9061 taatttggtc taaatcgtta agtggcccta cgcgcacacg atgccatacg cctttttcag
     9121 gaatatcagc cgtttgcacg acagcttcaa aaccttgcaa tgcaagtttc gcttttaagt
     9181 tatctgcttc ttcctcagta tgaaaagacc ctacttgcag ataataagtt ttattcgtgg
     9241 cgggctcatt gttggctttc aatgtgcttt caacttcttt gcttatttta ttttcatcac
     9301 ttggcaaaat cgtataaaag tcatagcttg tatcgtcatt attagcacta gcctcaggcg
     9361 agatgtcttt actggcggtt gcattttttt cttgagactt cgcatcctca gcaattttgt
     9421 ctgagagaga tttgctggtc tgcttattcg tttgaatcgc gaaagggtta tcgtctcttt
     9481 tgaggtaaac gacaaccgcg agcgaagcgg caatgcccag taaaatgcct attaacagtc
     9541 cagttaaaaa cgggctaccc tttttgctac gggtttgcct ttcggcgctt tgtttgtaat
     9601 ctttactcat ggtagataat gtccttacat tctttctttg gcggtgacgc ctagtaaatc
     9661 cagaccgttt ttaagcactt gctgtgtggc gtagattaag ctcaagcgag ccattttaac
     9721 ggtttcatcc tcaattaaaa acttatattc attgtaataa ctatgtaggt cactggctaa
     9781 atcttttaaa taatttgcaa ttacgtgagg ggcaagctca tttgcagcat tttctacagt
     9841 ttcagagtat gcgcttaagc gctgcattaa cgcagtttcg tgtggattat caagtgggga
     9901 aagatcagcg ctcaataaaa gtgatttgtc gccattccat tgcgttaata cactgcaaat
     9961 acgcgcatgg gcatattgaa tatagaaaac cgggttatcg ttggtttggg ctctagccaa
    10021 atcaatgtca aacactaatt gtgaatcagc gcggcgtgca gccaaaaagt aacgcgttgc
    10081 atcacagcca acttcttcaa ttaagtcacg tagcgtgaca taactcccag cgcgttttga
    10141 gagttttact tcttcaccac cgcgcatcac ggtgaccatt tggtgcaaca cataatctgg
    10201 ccaaccattg gggatgccaa tatttaacgc ttgcaggcca gcacgcacgc gggtaatggt
    10261 gctgtggtga tcagcgccct gctcgttaat tacgcgaaca aaaccacggt tccatttatc
    10321 ggtatgatag gcaatatcag ggacaaaata agtatagcta ccatccgttt tgcgcatgac
    10381 acggtctttg tcatcgccaa agtccgtggt ttttagccaa agggcatcat ttgcctcata
    10441 ggtatgccca ctgttgatga gtgcttgaac ggtatctttt accttacctg ttgtataaag
    10501 cgcgctttct agcgaaaaca catcaaattt gatttgaaac gcttttaaat ctaaatcttg
    10561 ttcatgacgc aaataagcaa cactaaaagc acgaatagaa tcaagattat taatgtcccc
    10621 gctggcggtg acttctatat cgtcggcaat gacggtgtgc ttggttaaat atgcggcagc
    10681 aatatcatta atgtactcac ctcggtaacc attttcagga aaactttcgt gttccgtaga
    10741 gatacccttg catctagcca gcacggagat tgtaagattg tcaatttgtg cgccggcatc
    10801 attgtagtaa aattcgcggg tcacatccca gccattagca tctaataacc ttgctaaaca
    10861 atctccaact gccgcaccac ggccgtgccc aacatgcaac gggccagttg ggttagctga
    10921 cacaaattca acctgaacct tttgcccttg accattttgg ttgcggccat attgatgccc
    10981 ggcttgcaga atatctttga ccactttttg ctgagaatgg ctgtttagaa aaaagttaat
    11041 aaaccctgca cctgcaatct ccgtttttgc aatatagtca ctgttaggca tagcagcaat
    11101 gagttgtgtg gcaatggcgc gtggattttg ttttaacggt tttgccaaaa caagtgctaa
    11161 atttgttgca aaatcgccat ggtctgctga ttttggtctt tcgagtaaaa tggctacctg
    11221 tagctcaggg gcaattgttt ttgcagcgtt ggcgagtaat tgggtaagat gtgttttcac
    11281 aataattgca ttaaatgcta aaagcgctat tttaacctac tgccgttatt ttttcttgaa
    11341 acaattgttt agtacactat attagaaaca ccactttgcc aaaatcaatc gtaaaagttg
    11401 ctctggacgt tccgttaaat cgcttatttg attatttatc tgacaatagc gagataagaa
    11461 ttggtcagcg tgtgcttgtg ccatttgggc gcagaaatca aattggcatt gtcttgagcc
    11521 agagtgattc cagcgattat ccaattgaaa agttaaagtc tgtaaagcag gtttttattg
    11581 atgaaacgcc gctggatgca gagttattaa aactcattcg atttagcgcg gactattatc
    11641 aatacccgtt agggcaagcg ctgctgtcag cacttcctgt acgtttgcgg caagtggccc
    11701 ctgctgtcgc gcgtaaacag tttatctata gtttgactga cttagggcgc agtcaaactg
    11761 ttgcacagct tcctaagcga cagttagtga cacatcgtgt gtttagcgcc ttgttggcaa
    11821 gcaatacggt atctgaagct gagctagaca caatatccag tagtgctcga aaagcggcca
    11881 agcaattggt ggcaaacggc tgggcaagcg ccaagcaagt ccatttgtta cagcgggtgg
    11941 ctgaaatacc cacggctcaa gtgcctgaac tcaatgatga gcaacaatcg gctgtagccg
    12001 aggtgataaa agattcacat cactttaagt cgtggttatt gcacggtatt acgggcagcg
    12061 gtaaaacaga agtttatatt cgcttaatgc agcatgtgtt aatgcaaaat gaagcacaag
    12121 tattggtgtt ggtgcctgaa attaacctta ccccccagct agaggcacgc tttagaagtc
    12181 gtttttctgg cattccgctc gtgagcttac atagtcattt gagcgaaagc gagcgcttac
    12241 atcactggca gttggcgcaa tcaggcgtgg ctagaatcat tattggtacg cgactttcgg
    12301 tttttacgcc gttgccgcat ttgaagttaa tcatcatcga tgaagaacat gatggctcgt
    12361 ataagcagca agatagcatg cgctaccacg cgcgggatgt tgcactggtt cgtgcaaaat
    12421 ggctgaatat tccagttgta cttggttcag caacgccctc gttagaaagt tggcacaatg
    12481 ccacggtgac agccgctgga ctaaaggcta ataaatatca cctcttaagc cttaaccaac
    12541 gtgcagttac ggcggcacaa ttaccaatca ttcattgtat agatactacc aaagtgaatc
    12601 tgcagcacgg tttcacaccc caactgatag aagcactaaa acttaggata tcgcgtggcg
    12661 agcagagttt gttgtttatc aaccgccgtg ggttctcgcc agtattatta tgttctgcgt
    12721 gtcactggat agcgccctgt acccgctgta gtagccgatt ggtggtgcat ttggggcagc
    12781 gaaaattacg ctgccatcac tgtggatacg agcaaaaaat tccgaatcaa tgcccaagtt
    12841 gtggcaatgc agacttgcac ccaacggggc atggcacgca acgcttagag cagactctgg
    12901 cacagttgct acccacagcg cgaatcgcac gtgttgataa agacagcatt agtaaaaaaa
    12961 atgccttggt agaaatttta gataaagttc accgtcaaga aatagatatt ttggtgggca
    13021 cgcagatgct tgccaaaggc catgactttc ctaatttaac attagttggc gtgattgatg
    13081 cagatagcgc cctgcatagc cccgatttta gggcaagcga acgattgttc gcacaattga
    13141 tgcaagtggc tggacgtgct ggacgtgctg ataaaacagg tcaagtcatt atccaaacac
    13201 agtttcctgg acacgtatta ttcaatgcgt tgcgaaggca agattacgca agttatgcaa
    13261 atgctttgtt gcatgagcgc caacaaatgc aattcccacc ctatattttt atggcattat
    13321 tgcgtgcgga agccaatgac tttaatttgg tacatcagtt cttaagttat gcatttaaag
    13381 ttgctagaga tttaaatact aaagtggtag tttatgaccc gattcgccca caaatggaac
    13441 gtttgaaagg catggagcgt ggctatgtgt tgatgcaagc tggcaataga ggcgtcttgc
    13501 aaaagttact taatagattg gtagcacaat taagaggaca tactgcatct gctaaggtta
    13561 ggtgggcgat agatgttgat ccattagaat tctgaaaact cgcacaagct cataataatt
    13621 tttttgtata aactaataca aaatatgtct ttgacgagct aaatttaacg gattagtatc
    13681 attcaaacga aaccatagtt aaggatgaat gaaatgacta ccttttcaag gcacttgtac
    13741 acgcccatac ttttcgcgtt gctaggtttg agcaatgcgt gtttagcgca ggcagacgat
    13801 aaacaacctg attccgctaa atcagtggtt gagcaaattg ttgacgtgca aactaaaata
    13861 gccagcggcc cgcataaagg atttcgcgcg aatcatgcta aaggaatcat tgcgacgggt
    13921 atttttacgc catctgccga agcggcaggt ttaaccagcg cagtccattt gcaaaacact
    13981 gccagcgaag tcattgtgcg gttttcaaat gcaacaggca ttccaaccat tgctgacacg
    14041 agcccaaagg ctaatccacg tggcattgca attcgtttta aacttgcgga tgaagcttat
    14101 acggatattg tcagtttgtc ggttaatcgg tttccggttt ctacccctga agccttttta
    14161 gccctgttgc aggcaaatgc cgcaacaaaa gctgattcac ctaaacctac cccaatcgaa
    14221 caatttttag cagaaaaccc tagcgcgcag gcttttgtga aaataccaaa gccagcaccc
    14281 gccagttatg ccacgcagtc ctattttggc attaatgcgt ttaaatttac caatgccaag
    14341 ggtgagtcgc gctatgggcg ctacctcatt acgccgcttg ccggaaatat cgtgctggat
    14401 gaagctaaag ttgccagcgc ttcgcctgat tacctctctg aggaattggc agcgagatta
    14461 aatcagggcg aagccagttt tcgcattagt gtgcaggttg ctaatgatgg cgacagtatc
    14521 aatgacccca ccgttgtttg gcctgaagat agggcgcaag ttgcacttgg gacgttgaca
    14581 ttaaaagcga ttccagcaga cagcaaaacc atagagcaaa aaattacatt taacccactc
    14641 attctgcctg atggcatcgc gccatcagag gataaaatat tacttgagcg gcctgcggcc
    14701 tatgcagtga gtgcttcacg ccgataaatc aaagctgata aatcaaacaa gtcgcctaga
    14761 gaaatttagg cggcttcaaa cataattcaa ttgactccgc gactgtgtcg tcacctgcaa
    14821 aatgattcgg gtataattgt acccttacgc gggcgtcgta taatggtaat accctagctt
    14881 cccaagctag agccgtgggt tcgattccca tcgcccgctc caaataaatt attcaccccg
    14941 tcttatgcgg tctaaaatca cataaatatc atatatttaa gccaaaatac cagcctatca
    15001 cgacttatcc tgtactatat tgtccattta caaaacgtat aaaataacgt ataaaagcaa
    15061 taccgattta ttttttatac gttatttatg aaaaaactaa ccactagcgc cctggatgat
    15121 ttaacaatca aagcggccaa agccaaagag aaagcctaca cgctaagaga cggtaacggc
    15181 ttgtttttgc tagtgcatcc taatggaagc aagtacttcc agttacgcac taccctgcat
    15241 ggcaaagcaa agctcataca gcttggcacg taccctattc tatcgctaag tgaagcccgt
    15301 gacctggggc gtgaaaaact caaaaaagta aaagtcgagc aaatagaccc agttatagag
    15361 gccaaattac taaagcacca aaaggctaag gatgccagca acatatttga agatgtggcc
    15421 aatgagtggt tagctattaa gcagcgtact ctagcgccca ctagccacac aaaaataaaa
    15481 cagacgttta atgctaacgt atttggggtg cttggcaagt accctatcaa agacattgac
    15541 aatcaaatgg tgcgcaaatg cctcatgatt atgcaaaagc gtggtgcgct cgagctgatg
    15601 gagcaaacaa gggcatggat taaacaggtg tttgaatttg ccctaagcga taacctgata
    15661 gctgaaaacc ctatcccatc gattgatgtc aggctagaaa agcacaaccc agaaaaatac
    15721 ccacgtttac aaagccggca agatgctggc accctactcc gcaatatcac taaatataag
    15781 ggtagctttg aggtgatcac ctgcgtgtat ttacaaatgc actttgcaca aaggcctagt
    15841 gagttaaggt gtgctaaatg gtcagagttt gacctggaca atgctgtttg gactattccg
    15901 ctgccacgat ctaaaacacg taagcacatg actaaacctc atacagtcat gctatcaagg
    15961 caggcgggga aaatgtgctg gcggttaaaa tc
//
LOCUS       DAOQSI010000028        15880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_2781_length_15880_cov_6.045118, whole genome shotgun sequence.
ACCESSION   DAOQSI010000028 DAOQSI010000000
VERSION     DAOQSI010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 15880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15880
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2781_length_15880_cov_6.045118"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="PKL42_03250"
     CDS             complement(<1..705)
                     /locus_tag="PKL42_03250"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNU66119.1"
                     /translation="MLGNWIKTFVLMAAITALFGVVGAALGGQAGMLLALLFAAAMNV
                     YAYWFSDKAVLKMYGAQEVSAENNYGDGQFTKYYHMVKELAENAQLPMPKVYVMNETQ
                     PNAFATGRNPEHAAVAATVGIMRALSERELRGVMAHELAHIKHRDTLISTISATVAGA
                     ISSLAQFGMFFGGARGGDGERSGSPIVSMLMMFLAPMAASLIQMAISRSREFEADRGG
                     AEISKDPQALASALEKI"
     gene            complement(798..1721)
                     /gene="fmt"
                     /locus_tag="PKL42_03255"
     CDS             complement(798..1721)
                     /gene="fmt"
                     /locus_tag="PKL42_03255"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNU66120.1"
                     /translation="MKIIFAGTPEFAVPALAALIAAGHEIVMVLTQPDRPAGRGMKLQ
                     ASPVKQLALQHQLHVFQPESLKPADVQAQIAAIGADVMIVAAYGLIIPTSVLHMPKKG
                     CYNIHASLLPRWRGAAPIHRALLAGDHETGVTIMEVVPALDAGAMVSKGVIPITESDT
                     TQTLHDALAQLGAELMGKAMQKLAEDGALPSTPQDERLVTYAHKLEKSEAAIDWRKSA
                     VEISRQVRGFNPFPVATAHLRGMTCRIWFANAAAGDGAIGEIRNLGETISVACGQGVL
                     HIYELQMPGGKRQSAKVFAQSQQLKAGEFFS"
     gene            complement(1742..2242)
                     /gene="def"
                     /locus_tag="PKL42_03260"
     CDS             complement(1742..2242)
                     /gene="def"
                     /locus_tag="PKL42_03260"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNU66121.1"
                     /translation="MAVLNILNYPDPRLHTVAKLVKEVDANIRRLIDDMTETMYAAPG
                     IGLAATQVNQHIQLLIIDTSETKDELQVFINPKIIEKNGERAFEEGCLSVPGVYESVT
                     RAETVKVEALNRHGEKFTLHAEGLLSVCIQHEMDHLLGKVFVEYLSPLKRSRIKNKML
                     KLSRQK"
     gene            2413..3564
                     /locus_tag="PKL42_03265"
     CDS             2413..3564
                     /locus_tag="PKL42_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNU66122.1"
                     /translation="MFRHIITLLMFCCISLTANAQEVQLNSNHPDRHVVKKGDTLWGI
                     SGKFLKDPWLWPKVWQFNRAEIKNPHLIYPGDVVVLDRSGKNPRLRLLRETVTLQPGA
                     VEEPLDKEAISTIPLSVISPFLNQPLVIEKDQLATSPRIIGAQDNRVVLSPGTRVYIN
                     DLNEGDGLDWFVYRPGKNLVDPDTKEVLGIEANYLGNARVTKYGEPASADITKAKEEI
                     FTKDRLVVSGDEVTTNFVPRAPESVITGRIMHIYGGVAEGGPQSIVSLNRGSTDGVEV
                     GHVLAINRYGRVIKDPEYQKDKNAPSKPKLKELNFDVTTGPDGKKIVNFEKEPVDPSG
                     KIVLEPGMVKLPDERVGLLMVFRVFDRVSYALVMQASEPVYELDAVTTP"
     gene            3577..4692
                     /gene="dprA"
                     /locus_tag="PKL42_03270"
     CDS             3577..4692
                     /gene="dprA"
                     /locus_tag="PKL42_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNU66123.1"
                     /translation="MYSGEDIAEKTLWIRLSSIEGIGSQTFCQLLKEFGSPANVFSAT
                     FKQLTAIVSEKIALSIKQDFNEDSLKTSQNWLMQANNHLVTLADAQYPQLLLEINDPP
                     PFLYAKGNLALLNQPSIAIVGSRNASVQGEKNAEAFAEGLCHHGLCVVSGLALGIDGA
                     AHRGALKANGATIAVVGTGLDIVYPAKHRDLAHHIVEHGLIISEFALGTPSKPQNFPK
                     RNRIISGLSLGCLVVEANLQSGSQITARLAGEQGREVFAIPGSIHSPMSKGCHQLIKQ
                     GAKLVDDLQDIVEELGLSRQLNSQISTTANHDNAANTQTGSHQTLLDLMGFEPIALDR
                     LVHLSDLTVSEVSSMLMLLELEGSVASIAGGNFQRIV"
     gene            4814..5287
                     /locus_tag="PKL42_03275"
     CDS             4814..5287
                     /locus_tag="PKL42_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HNU66124.1"
                     /translation="MFEVLVYMFENYIDTHARPDEYTLSKELVAAGFDEQDINGAFHW
                     FNQLERKTSQPDIFQPPKFTSTRIFTEDELKKVTLESIGFILFLEQANILNSAQREII
                     LELAMHLPQAEISVDEIRWIVLMTTWGAGQSGKDHTKDYLFIEDVLMSKQKPTLH"
     gene            complement(5496..6332)
                     /gene="metF"
                     /locus_tag="PKL42_03280"
     CDS             complement(5496..6332)
                     /gene="metF"
                     /locus_tag="PKL42_03280"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HNU66125.1"
                     /translation="MNNNIPLSFEFFPPKTAEGIDKLRETRQQLAKFDPEFFSVTFGA
                     GGSTRDRTMDTVLEIQAEGFSAAPHISCISSSKSEIRELLHAYQSHGIKRLVTLRGDV
                     PSGEVSSGDFRYALELVEFIRAETGNYFHIEVAAYPEFHPEARSPAADLLNLKRKVEA
                     GANSAITQYFYNADAYFRFIDQCATANINVPVVPGIMPIYNITQLARFSSVCGAEIPR
                     WLKMRLEEYGDDLQSLRAFGIDVVSELCANLLESGVPSLHFYTLNQAGIISNIIENIR
                     AE"
     gene            complement(6371..6715)
                     /locus_tag="PKL42_03285"
     CDS             complement(6371..6715)
                     /locus_tag="PKL42_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNU66126.1"
                     /translation="MKLLLAWVLNALALLAVAYLIPEIHVANFITALIAAVFIGLANI
                     LIKPILTVLTLPITIITLGLFILVINGLLFYAVGNLLQGFEVTTFTAGIIGAIAYSII
                     SWLLSALLIEKD"
     gene            complement(6737..8146)
                     /gene="ahcY"
                     /locus_tag="PKL42_03290"
     CDS             complement(6737..8146)
                     /gene="ahcY"
                     /locus_tag="PKL42_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNU66127.1"
                     /translation="MNAVTNFNDYIVADINLAAFGRKEIAIAETEMPGLMAIRQEYAK
                     AQPLKGARITGSLHMTIQTAVLIETLKDLGAEVRWASCNIYSTQDHAAAAIAAGGTPV
                     FAIKGETLTEYWDYTHRIFEWTDGGYSNMILDDGGDATLLLHLGTRAEKDLSVIAKPT
                     SEEEICLFDAIKAKLKTDPTWYSTRLAKIIGVTEETTTGVHRLYQMHKEGKLAFPAIN
                     VNDSVTKSKFDNLYGCRESLVDAIKRATDVMIAGKIAVVAGYGDVGKGSAQALRALSA
                     QVWVTEIDPICALQAAMEGYRVVTMDYASAHGDIFVTATGNYHVITHDHMAKMKDQAI
                     VCNIGHFDNEIDVASLEKYEWDEIKPQVDHVIFPDGKKIILLAKGRLVNLGCGTGHPS
                     YVMSSSFANQTIAQIELFTQTDKYPVGVYTLPKHLDEKVAVLQLKKLNAQLTTLTDAQ
                     ADYIGVSKSGPYKPDTYRY"
     regulatory      complement(8232..8349)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            complement(8425..9588)
                     /gene="metK"
                     /locus_tag="PKL42_03295"
     CDS             complement(8425..9588)
                     /gene="metK"
                     /locus_tag="PKL42_03295"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNU66128.1"
                     /translation="MNTYLFTSESVSEGHPDKLADQVSDSILDAILAQDPTARVAAET
                     LANTGLIVLAGEITTTANVDYIKVARDAIKRIGYDNTEYGIDYKGCAVLVAYDKQSPD
                     IAQGVDNANDDPLDQGAGDQGLMFGYAVNETPQLMPLPIWLSHRLMERQAQLRKDGRL
                     PWLRPDAKSQVTIQYVDGKPHKIDTVLVSTQHAPEMSLEAIREATIEEIIKPMLPKEL
                     IKGEIKYLVNPTGRFVVGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRS
                     AAYAGRYVAKNIVAAGLADKCLVQVSYAIGVAQPTSVMVETYGTGKVSNEVLTALVLA
                     HFDLRPKGIVKMLDLLRPIYAKTAAYGHFGRDEPEFTWEATDKAATLRAAAGL"
     gene            10024..10848
                     /gene="dapF"
                     /locus_tag="PKL42_03300"
     CDS             10024..10848
                     /gene="dapF"
                     /locus_tag="PKL42_03300"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNU66129.1"
                     /translation="MNLNFTKMQGAGNDFMVIDAIHQNVALTPAQIKSLAHRQFGVGF
                     DQLLLVEPSTIADFKYRIFNADGSEVSQCGNGARCFVRFVVDQHLTNKTEISVETASG
                     IITPKLEANGLVTVNMGAPKFAPNEIPFIADNIATSYTLNVGEQNLTIAAVSMGNPHA
                     VQIVDDVETAPVVALGSQIEVHPRFPERVNAGFMQVIDPHHIKLRVFERGSGETLACG
                     TGACAAVVTGIQLGRLQSPVNVTARGGELHIAWLGVNSPVMMTGPAVTVFTGSLTL"
     gene            10885..11580
                     /locus_tag="PKL42_03305"
     CDS             10885..11580
                     /locus_tag="PKL42_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HNU66130.1"
                     /translation="MQQDNQNLNTPAQQQSISDDQVTSYLRSHPHFFEHHASLLTEIY
                     LPSPHGAGAISLAERQQLAQRDKIRVLEVKLASLIGYAQENDATSEKIHALCVNLLKN
                     QGFENLQNLIAKSMQDDFAVTQSAIRIWANPDSPQPTEFTPENKAFIEWVLTLNAPHC
                     GALSDAAKGMLNDHLASFAFIPLYQNSAEKQAFGVLMLGAADAERFKADMGLMYLARI
                     GEFVSASLLQYIE"
     gene            11600..12481
                     /gene="xerC"
                     /locus_tag="PKL42_03310"
     CDS             11600..12481
                     /gene="xerC"
                     /locus_tag="PKL42_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HNU66131.1"
                     /translation="MPHLDDYLQHLTFERGLSTNTLKSYTRDIQLLQGLAQESDLANI
                     QNTQIRRFIATLHSRGLSSKSIARALSAWRGFFDYLIHHKGFTQNPVVGLRAPKSAKT
                     LPQALSTDQAIQFVDIQGDGLLETRDHAILELFYSSGLRLSELVNLDTDRLDFSEGTV
                     TVTGKGNKTRIVPMGSHSMNAIQTWLQHRARIQIAEANPNALFVTQQGRRITPRAVQY
                     RVKEWAIKQGVNTDMHPHLLRHSFATHVLQSSQDLRAVQEMLGHANISTTQVYTHLDF
                     HHLAKIYDTAHPRAKKK"
     gene            12572..13156
                     /locus_tag="PKL42_03315"
     CDS             12572..13156
                     /locus_tag="PKL42_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HNU66132.1"
                     /translation="MEHTLPALPYAKNALVPHISEETFEYHYGKHHQAYVTNLNNLIK
                     GTEFENASLEDIIKKSSGGIYNNSAQVWNHTFFWNSLKPNGGGAPTGALADAINKKWG
                     SFDEFKKAFQASAVGNFGSGWTWLVKKADGSVDIVNMGAAGTPLTTGDKALLAIDVWE
                     HAYYIDYRNARPKFVETFLTSLANWDFAASNFAA"
     gene            13258..13605
                     /locus_tag="PKL42_03320"
     CDS             13258..13605
                     /locus_tag="PKL42_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66133.1"
                     /translation="MSPNYETLSDAEKYFIVILLALYAGTFLIGIMFLGEVPCMLDLE
                     GECKFGTYFKQLLPFYGFTFALNIAIVMSAYYLNKLAKKSIFIFRGLILPPLLALFYY
                     LYHYGSNFVRALF"
     gene            13641..13955
                     /locus_tag="PKL42_03325"
     CDS             13641..13955
                     /locus_tag="PKL42_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66134.1"
                     /translation="MLQRIVIQFLFIFLFAFTQIGVVTHEISHFSDAAKHTQQDKNTS
                     AEQCGQCISYAQVASGLQPHAFELHDFITSFELNSSKRFNLQPSLLTAYAARAPPQNI
                     SI"
     gene            14018..>15880
                     /locus_tag="PKL42_03330"
     CDS             14018..>15880
                     /locus_tag="PKL42_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNU66135.1"
                     /translation="MKKHHLKQVRPAFALNTLFLALSCLFIENAKAEAPLPTDTTAFE
                     LSTISVTGNPLGVGSDALVIPISILNGRELANRRESTLGDTLNGIPGVAATHFGPNAS
                     RPVIRGLDAERVRIMQNGVGVLDASSLSFDHAVTIDPLIIEQIDVVRGPAALLYGGSA
                     VGGVVNAIDHRIPKESFDGVTGRVEGSIGGAESQNNLAGVVDVGYGALVIHADAYTRK
                     TSDTDIDGYAVSSRKSRADGTPRENRGKLVNSSASGEGGALGASFIFDNGYLGASYSA
                     FRNNYGTVAEEAVRADMRSDRVDVATEFRDLGNVINRVKARMAYTDYQHQEIEDGEVG
                     TTFKNEGVEGSLEFGHAKIGNLEGVLGFQFQNTDFEALGDEAFVPGVNTQNKALYLYE
                     ELPIDQLKLSFGGRVERVNVDSRNNAKFGAGQNNSFTPYSYALGGLYTFNPNWSLATN
                     LSHNERAPSYFELYADGAHLATGQYEVGNTNFDKERSNGIDAQIRWKDGKNSFSVGAY
                     YTRFSRFLGLLDSGNTRGIDGELNPDDLDGDGVADLSGEDILPESQFSMFAATFKGLE
                     AEGKFNLVDNLDLTLRGDYVRATNRDNGQALPRITPLRLGAGLRYQKNALGARVD"
BASE COUNT         4319 a         3713 c         3522 g         4326 t
ORIGIN      
        1 aattttctct aaagcactgg ctaacgcttg cgggtcttta ctgatttctg cgccgccacg
       61 gtcagcctca aactcacggc ttctcgaaat cgccatttga ataagcgagg ccgccatcgg
      121 cgccaaaaac atcatcaaca tacttacaat ggggctgccg ctacgctcgc catcaccgcc
      181 tctagcgccg ccaaaaaaca taccgaattg tgcaagtgaa gaaatcgccc ccgcaacggt
      241 ggcagaaatg gttgaaatta atgtatcgcg atgtttaatg tgtgcaagtt catgcgccat
      301 cacaccgcgt aactcgcgct cacttaaagc ccgcatgatg cccaccgttg ctgcaaccgc
      361 agcgtgttca ggattgcggc ctgttgcaaa ggcgttaggc tgcgtttcgt tcatcacata
      421 gacttttggc attggtaatt gggcgttttc agccagttcc ttcaccatat ggtagtactt
      481 ggtaaattga ccatcgccat aattgttttc tgctgaaact tcttgtgcgc catacatttt
      541 gagcaccgct ttgtctgaaa accaataggc atatacattc atggccgccg caaacaacaa
      601 tgccaacagc atgcccgcct ggccacctaa tgccgcacca accacaccaa acaatgcggt
      661 gatggctgcc atgagtacaa aagttttaat ccaattgcct aacataagca tctcctgatt
      721 cttttaaaaa acgtgcttat ctaatatgct gcttaatgcc aaaaaattca agaaaaaatc
      781 agccgagatt acaaagttta actaaaaaat tcccccgcct tgagttgctg cgattgcgca
      841 aacactttag cactttggcg cttgccgccc ggcatttgta gctcgtaaat gtgcaaaacg
      901 ccttgcccgc atgccacgct aattgtttcg cctaggttgc gtatttcacc aatcgcgcca
      961 tcgcctgctg cggcatttgc aaaccagatt cgacatgtca ttccgcgcaa atgcgcggta
     1021 gcaacaggaa aagggttaaa tccgcgcact tgccgtgaaa tttcaaccgc agatttacgc
     1081 caatcaatgg ccgcttcgga tttttcaagc ttgtgcgcgt aagtgaccaa tcgctcatct
     1141 tgcggtgtgc tgggtagcgc gccatcttca gctaattttt gcatggcttt acccattaac
     1201 tcagcgccaa gctgtgctaa tgcatcgtgc aacgtttggg tggtgtcgct ttctgtaatc
     1261 ggaattacac ctttgctgac catcgcgcct gcgtctaacg cgggtacaac ttccataatg
     1321 gtcacacctg tttcatggtc ccccgccagc agtgcgcgat gaatcggtgc cgcgccgcgc
     1381 caacgtggta aaagtgaggc gtgaatgtta tagcaaccct ttttgggcat gtgcaatacg
     1441 gacgttggaa taatcagccc atatgcagcc acaatcatga catcagcccc tatggcagca
     1501 atttgcgcct gcacgtctgc gggctttaag ctttctggct gaaaaacatg caactgatgt
     1561 tgcaaagcta attgcttaac agggcttgct tgcaacttca tgccgcgccc tgcggggcgg
     1621 tctggctggg tgagcaccat cactatttcg tgcccagccg ctattagcgc tgcaagcgct
     1681 gggacggcaa attctggagt gcctgcaaat ataatcttca atgattgcgc cttttgggtt
     1741 gttatttttg acggcttaat ttaagcattt tgtttttgat gcgactgcgt tttagcggtg
     1801 acaaatattc aacaaacacc ttacccaaca gatggtccat ttcgtgctga atacacacac
     1861 ttaatagccc ttcagcatga agagtgaatt tttcaccgtg gcggtttaag gcttcaactt
     1921 taacggtttc agctcttgtc acactttcat atacaccagg cacagacaaa cagccttctt
     1981 caaaggcacg ttcaccgttt ttttcgatga tttttggatt gataaatacc tgcaactcgt
     2041 ctttggtttc gctggtgtcg ataatcaaca actggatatg ctgatttact tgcgtagcgg
     2101 ccaagccaat accgggcgct gcgtacatgg tctcagtcat atcatcaatt aaacgccgaa
     2161 tattcgcatc cacttctttc acaagcttgg caaccgtgtg cagccgcgga tctggataat
     2221 ttaaaatatt taaaactgcc ataaagtgtc tttattttga taaaaagtgt aaatttgttc
     2281 aaaaaaatca aggcattttt taaaaaagct tgattgttta aaggattacg tgataaattt
     2341 aacgtagttt cgcggtttgt tcatgtgttt ttaccgccta tttttaatgc attttcgact
     2401 gaggtcttca gtatgtttcg tcatattata acgctgctca tgttttgttg cataagcttg
     2461 actgcgaatg cgcaggaagt tcaattaaat agcaatcatc cagaccgaca tgtcgttaaa
     2521 aaaggtgaca ccctgtgggg catttcaggc aagtttctga aagacccttg gctgtggcca
     2581 aaagtttggc aatttaatcg cgctgaaatc aaaaacccac atcttattta ccctggcgat
     2641 gtcgtcgtac ttgaccgtag cggtaaaaac ccgagattgc gcttattacg tgaaaccgtc
     2701 accctgcaac cgggggccgt ggaagaaccg ctcgataaag aagccatttc aaccattccg
     2761 ctcagtgtta tcagcccatt tttaaatcaa cctttggtga ttgaaaaaga ccaactggcc
     2821 acttctccca gaattattgg tgcccaagac aaccgcgtgg tgttaagtcc cggcacacgt
     2881 gtctacatta atgacctgaa tgaaggcgac ggccttgatt ggtttgtgta tcgtccgggt
     2941 aaaaacttag ttgaccctga caccaaagaa gtattaggca ttgaagcaaa ctacttgggt
     3001 aacgcaagag ttactaaata tggcgaacca gccagtgcag acattacaaa agcaaaagaa
     3061 gaaattttca ccaaagaccg gttagtggtg agtggtgatg aagtgaccac caactttgtt
     3121 ccccgcgcac cagagtccgt gattactggc cgcatcatgc atatttatgg cggcgtggct
     3181 gagggcggcc cacaaagcat agtcagcctc aaccgcggta gtacagatgg cgttgaagtt
     3241 gggcatgtgc tggcaattaa tcgttatggc cgcgtgatta aagaccctga gtatcaaaaa
     3301 gacaaaaacg cgccatcaaa acccaagtta aaagaactta attttgatgt gaccactggc
     3361 cctgacggca aaaaaattgt caattttgaa aaagaacctg tagacccaag cggcaaaatt
     3421 gtgctcgaac ctggcatggt gaagttacct gatgaacgcg ttggcttgct catggtgttc
     3481 cgtgtgtttg accgcgtgtc atacgcgctg gtgatgcagg catcagaacc tgtttatgag
     3541 ctagacgctg ttactacccc ttaaataatc taaagaatgt acagcggcga agatattgca
     3601 gaaaaaacgc tgtggattcg tttaagcagt attgagggta ttggttcaca aactttttgt
     3661 cagttgttaa aagagtttgg cagcccagcc aatgtatttt ctgcaacttt taagcaactg
     3721 acagcgatcg tctcagaaaa aatcgcgctt agtatcaaac aagatttcaa tgaagacagc
     3781 ctgaaaactt cacaaaattg gctgatgcaa gccaacaatc atctggttac gcttgcggat
     3841 gcgcaatacc ctcaactttt acttgaaatc aacgacccgc caccgttttt atacgctaaa
     3901 ggtaatcttg ctttactcaa ccaaccgagc attgccattg tgggtagccg caacgcttct
     3961 gtgcagggcg aaaaaaatgc tgaagccttt gctgaagggc tatgtcacca cggtttatgc
     4021 gtggtgagtg gcttggcact tggtattgat ggcgcggcac accgtggtgc gctcaaggca
     4081 aatggtgcga ccattgcggt ggtgggtaca gggcttgata ttgtctaccc tgccaaacat
     4141 cgtgatttgg cgcatcacat tgtcgagcat ggtttgatta tttctgaatt tgccttgggc
     4201 acaccatcta agccgcaaaa ttttccaaag cgcaatcgca ttatcagcgg cttgagtttg
     4261 ggttgtttgg tggttgaagc taatctgcaa agcggctcac aaattaccgc acggctcgcc
     4321 ggcgagcaag gacgtgaagt gtttgccatt cccggttcca ttcactcacc catgtctaaa
     4381 ggctgccatc aactcatcaa acaaggcgct aaactggtag atgatttaca ggatattgtt
     4441 gaagagctgg gcttatctcg ccagcttaac agccaaatca gcactactgc caaccatgat
     4501 aatgcggcaa acacacaaac tggcagccac caaactttac ttgatttaat gggctttgaa
     4561 cccattgccc ttgaccgctt agtccatttg agtgacttga ccgtcagtga ggtttcctcc
     4621 atgcttatgc tattggaatt agaaggaagt gtggcgagca ttgcgggtgg aaattttcaa
     4681 cgcattgtct aattaaaacc gcgcttcacg cagtgtagat gttggcaatt tgaacttgcc
     4741 gcgcttcatt aagctaatat ccatgcacgc gacacgcaag tatttaaata atgattttgg
     4801 gtgataaaag acaatgtttg aagtattagt atatatgttt gaaaactaca ttgatacgca
     4861 tgccaggcca gacgaatata cactttcaaa agagctggtt gctgctgggt ttgatgagca
     4921 agacatcaat ggtgcgtttc attggtttaa tcagcttgag cgcaaaacga gccaacctga
     4981 tatttttcaa ccgcccaaat ttaccagcac acgtattttt accgaggatg agcttaaaaa
     5041 agtcacctta gaaagtatcg gctttatttt gtttttagag caggccaata ttttaaattc
     5101 tgcacagcgc gaaattattc ttgagttagc catgcattta ccgcaggctg aaatcagcgt
     5161 tgatgagatt cgctggattg tgcttatgac cacctggggc gctggccaat cgggcaaaga
     5221 ccacaccaaa gattacttat ttattgaaga tgttttgatg agtaagcaaa aaccaaccct
     5281 gcattaatag ctttcagcgt gcgaattttt tcgctaatgt ctaccgaagt ttaagcccta
     5341 aataaaccaa aaaaaaggct acataattcg ccaacctttt tcgatgccct gcaagccttt
     5401 taaataaagg ctcgcagagc atatatttgt tctcgggtga aaatgtgctt atttgagggt
     5461 tgttttaacc atgcaattta ctacgcaaaa atccgttatt ctgcgcggat attttcaata
     5521 atattactga tgatgcccgc ttgattgagc gtataaaaat gcaagcttgg cacgccgctt
     5581 tcaagcaaat tcgcacacaa ctcgctcacc acatcaatgc cgaacgcacg taatgactgc
     5641 aagtcatctc cgtattcttc caaccgcatt tttaaccagc gcggaatctc agcaccgcac
     5701 acgctagaaa agcgcgctag ctgtgtaatg ttataaatcg gcataatgcc aggcactacc
     5761 ggtacattga tattagcggt tgcacattgg tcaataaagc gaaaataggc gtctgcattg
     5821 taaaaatatt gcgtaatggc agaattggca cctgcctcca ctttgcgttt taagttcaac
     5881 aaatcagcgg ccgggctacg cgcttctggg tgaaactccg ggtaagcagc cacttctata
     5941 tgaaaatagt tgcctgtttc agcacgaata aactctacca attcaagcgc atagcgaaaa
     6001 tcgccactgc taacttcacc tgaaggtacg tcgccgcgca atgtgacaag acgcttgatg
     6061 ccatgcgact ggtaggcgtg caataattct cgaatttcag atttactcga tgagatgcac
     6121 gaaatatgcg gcgctgcaga aaagccttcg gcctgaattt ccagcacagt atccatggtt
     6181 ctgtcacgtg ttgagccgcc tgcaccaaag gtgactgaaa aaaattcagg gtcaaattta
     6241 gcaagctgtt ggcgtgtttc acggagctta tcgatgcctt ccgctgtttt tggcggaaaa
     6301 aactcaaaac tgaggggtat gttgttattc atttcattct tattcaatgt aattgcctgc
     6361 gagccgttga ttaatctttt tcaatcaata gcgcacttaa aagccacgaa atgatgctgt
     6421 acgcaatggc accaataata ccagcggtaa aggttgtgac ttcaaaacct tgcaacaaat
     6481 taccaacggc ataaaacaat aagccattga tcaccagtat aaaaagcccg agcgtaataa
     6541 tagtaatggg cagtgtcagc accgtcaaaa taggtttaat cagaatattg gcaaggccga
     6601 taaaaactgc cgcaattaac gcggtaataa aattcgccac atgaatctca ggaattaaat
     6661 atgcaaccgc taacagcgct aaagcattta atacccatgc taataataat ttcatgtggc
     6721 gactcctttt ttgaacttaa taacgataag tatctggttt atatgggcct gatttagata
     6781 cgccaatata atctgcttgt gcatcagtca gtgtcgtcaa ttgtgcattt aattttttca
     6841 attgcaaaac ggcgactttt tcatccaaat gcttaggcaa agtataaacg cctaccgggt
     6901 atttgtcagt ttgcgtaaac aactcgattt gcgcaatggt ttgattggca aaacttgagc
     6961 tcatgacata acttgggtgg cctgtgccac aacctaaatt caccaaacgg ccttttgcca
     7021 acaagataat ttttttacca tcagggaaaa tcacatggtc tacttgtggt ttgatttcgt
     7081 cccactcgta tttttcaagg cttgctacgt caatctcgtt atcaaaatgg ccaatattgc
     7141 acacaatggc ctggtctttc atttttgcca tgtggtcatg tgtaatgaca tgatagttac
     7201 ccgttgccgt cacaaaaata tcgccatggg ctgaagcgta atccatggtg acgacacggt
     7261 agccttccat ggccgcctgc aatgcgcaga ttgggtcaat ttctgtcacc cacacttgag
     7321 cagaaagcgc acgcaacgct tgcgcagagc ctttacccac atcaccataa cctgccacga
     7381 ctgcaatttt tcctgcaatc atcacatcgg tagcgcgttt aattgcatca accaagctct
     7441 cacggcagcc atataagttg tcaaattttg attttgtcac tgaatcgttc acgttaatgg
     7501 ccgggaaagc gagtttgcct tctttatgca tttgatacaa acgatgcacg cctgtagtgg
     7561 tttcttccgt cacgccaata atttttgcta accgggttga ataccaagtt gggtcagttt
     7621 taagcttggc tttaatcgca tcaaacaaac atatttcttc ctctgaagtt ggttttgcaa
     7681 tcactgacaa atctttttct gcacgtgtgc cgaggtgcag taacaaagtc gcatcgccac
     7741 catcatccaa aatcatgttg ctgtagccac cgtcagtcca ttcaaaaata cggtgggtgt
     7801 aatcccaata ttcagtcagc gtttcacctt taatggcaaa aacaggtgta ccgccagccg
     7861 caatcgcagc agccgcgtgg tcttgcgttg agtaaatatt gcaagatgcc cagcgtactt
     7921 cggcgcctaa atctttgagt gtttcaatca gcactgctgt ttgaatggtc atgtgtaatg
     7981 agccagtaat gcgcgcgcct ttgagtggtt gcgctttggc gtattcttgg cgaatcgcca
     8041 tcaagcctgg catttccgtt tcggcaatgg caatttcttt gcggccaaaa gccgctaaat
     8101 tgatatcagc aacaatgtaa tcgttaaaat tagtcacagc gttcatcatt aaatccttaa
     8161 agtttcgtga ctgttgatga cgtacaaaaa gcaaaacggc gtttcacttt acttcaccga
     8221 acagtcggtt aataaaatga acgccgttac aagattgcca aacgctttta agttggcggc
     8281 ttgaaaaccg agcctagggg ccgatgcttt tgactacgcg catcaaccct cgcaacgttc
     8341 ctcggttaat tgtttgaatt atattacttt ttcaattcgc tacgcaaata cttaacatag
     8401 cgaattgaat ttcaacatta caaattataa tcccgcagct gcgcgtaatg tagccgcttt
     8461 gtcggtggct tcccaagtaa actcaggctc atcacgacca aaatggccat aagctgccgt
     8521 tttagcgtaa atcgggcgta acaaatcaag cattttaaca atgccttttg ggcgcaagtc
     8581 aaaatgcgct aacaccaatg ccgttagcac ctcattcgac actttacccg tgccgtaagt
     8641 ttctaccatc acagatgtgg gttgcgctac gccaattgcg taagaaacct gcaccaggca
     8701 tttatctgcc aagccagctg ccacaatgtt tttggcaaca taacgacccg cgtaggcggc
     8761 tgaacggtct acttttgacg gatctttacc tgaaaaagca ccgccgccat gtggcgccgc
     8821 gccaccgtaa gtatccacaa taatcttacg gccagttaag ccgcaatcgc cttgtgggcc
     8881 gcccacaaca aagcggccag ttgggttcac caaatattta atctcgccct taatcaactc
     8941 ttttggcaac atcggtttaa tgatttcttc aatcgtcgct tcgcgaatgg cctctaagct
     9001 catttctggc gcgtgttggg ttgaaaccag caccgtatca attttgtgcg gtttgccatc
     9061 tacatactga atcgtcactt gcgattttgc atctggacgc aaccaaggca ggcggccatc
     9121 tttacgcaat tgcgcttggc gctccatcaa acgatggctt aaccaaatcg gcaaaggcat
     9181 tagttgcggt gtttcattca cggcatagcc aaacatcaag ccttggtcac ccgcgccttg
     9241 gtctagcggg tcgtcattgg cgttatccac gccttgtgca atgtcgggcg attgcttatc
     9301 gtaggccacc aacaccgcgc aacctttgta atcaatgccg tattcggtat tgtcgtagcc
     9361 aatgcgttta atcgcatcgc gtgctacttt aatataatct acatttgcag ttgttgtgat
     9421 ttcacccgct aaaacgatca gccctgtatt ggctaacgtt tctgcggcaa cgcgcgcggt
     9481 cgggtcttgt gcgagaatcg catctaaaat actgtcggat acttggtctg cgagtttgtc
     9541 tggatgacct tcagaaacgg actctgaagt aaaaagataa gtgttcattt gtgcgccttg
     9601 gggtaaattt caaactttcg tgtctgaaaa tattagttaa aaatgaaatg atatttatca
     9661 ctaaaggcta aaggataaac atcaggtcaa catcggacag tcattctacc taatatattg
     9721 agactatgac caacaatctt gtcaataatt tgtaacggat atggattgat aacgtgatgt
     9781 ttttacattc attttgtatc gaatttttgt ccgagaaaat tttgatgctc tgcgagcctt
     9841 ataaataaag gctcgcagag catcaaaaaa ggttgggctt gtaaaagcac aataaaaccc
     9901 attttttttc tgaaaatatt cagcaatttt aatgaaaata aatgcgctta gttgccaaca
     9961 cttcaagcgc tgcttcacgt aaataaagtg ttatccgctg gcagttacaa ttataataat
    10021 cggatgaatt tgaatttcac aaaaatgcag ggcgctggca atgattttat ggtgattgac
    10081 gccatacatc aaaacgttgc gctgacgcca gcgcaaatta aatcgctcgc ccatcggcag
    10141 tttggggttg gctttgacca attattattg gttgaacctt caaccattgc cgattttaaa
    10201 taccgcattt ttaacgccga tggctctgag gtgtcacaat gcggcaacgg cgcgcgctgt
    10261 tttgtgcgct ttgtggttga ccaacactta actaacaaaa cagaaatcag tgttgaaacg
    10321 gctagcggca tcatcacacc caaactagaa gccaatggac tggtgacggt gaacatgggc
    10381 gcgcctaaat ttgcgccaaa tgaaataccc tttattgcag ataacatcgc gacgagttat
    10441 acgctaaacg ttggcgagca aaacttgacc atcgctgcgg tttccatggg aaatccacat
    10501 gccgtacaaa ttgtcgatga cgttgaaaca gcgccagtgg tggcgcttgg cagccaaatt
    10561 gaagtgcatc cgcgctttcc tgagcgtgtg aatgcgggtt ttatgcaagt gattgacccg
    10621 catcacatta agttgcgcgt gtttgagcgc ggcagtggag aaacgctggc ctgcggcacg
    10681 ggcgcttgtg cggcggttgt gacgggcatt cagttagggc gattacaatc gcctgttaac
    10741 gtgaccgcgc gtggtggtga actccacatt gcctggttag gcgtaaattc gcccgtgatg
    10801 atgaccggcc ctgctgtgac cgtattcaca ggcagcttaa cattgtaata aatgaagctg
    10861 taacaacaat ctagaaggat gaccatgcaa caagataacc aaaacctgaa cacgccagcg
    10921 caacaacaga gcatttctga cgaccaagtg acgagctatc tgcgtagcca tccacatttt
    10981 tttgagcacc acgcaagctt actgacagaa atctacttgc ccagcccgca tggcgcaggc
    11041 gcgatttcgc ttgcggagcg tcaacaatta gcccagcgcg ataaaattcg cgttcttgaa
    11101 gtcaaactgg ccagcctgat tggctacgca caagaaaacg acgcaaccag tgaaaaaatt
    11161 catgcgcttt gcgttaactt attaaaaaat cagggctttg aaaacctgca aaacctcatt
    11221 gcaaaaagca tgcaagacga ttttgcggta acgcaatccg ctattcgtat ttgggctaac
    11281 cctgattcgc ctcagccgac tgaattcacg cctgaaaata aggcttttat agaatgggtg
    11341 ctaacgctca acgcaccgca ttgtggcgcg ctgtcagacg ctgcaaaagg catgctcaat
    11401 gaccatctgg cttcttttgc ttttatcccg ctatatcaaa acagcgctga aaagcaagct
    11461 tttggcgttt taatgttagg cgcggctgat gccgagcgct ttaaagcaga catgggcctc
    11521 atgtacttag cgcgcattgg cgaattcgtc agcgcatcgc tactacaata tattgagtag
    11581 ttgtgcgcac cgctaaggaa tgccgcattt agacgactac ctgcaacatt taacttttga
    11641 gcgtggctta agtacaaaca cgctcaaaag ttacacccgt gacattcaat tgctacaagg
    11701 gttagcgcaa gagtctgact tagcaaatat tcaaaatacg caaattcggc gttttattgc
    11761 caccctgcac agccgtggct taagtagcaa atccattgcg cgcgcgctgt cggcatggcg
    11821 cggttttttt gactacctca tccaccataa gggcttcacc caaaaccctg tcgttggttt
    11881 gcgcgcaccc aaatctgcta aaacactacc acaagcgctc tccactgacc aggcgattca
    11941 gtttgttgac attcaaggcg atggcttact tgaaacacgg gaccacgcga ttttggaact
    12001 gttttactcc tctggcttgc gtttatcaga gctcgttaat ttagacacag accgcttaga
    12061 cttttctgaa ggcacggtaa cggtgacggg taaaggcaat aaaacgcgca tcgtgccgat
    12121 gggcagccat tcgatgaatg ccattcaaac ctggctacaa catcgcgcgc gcattcaaat
    12181 tgccgaggca aaccccaacg cactgtttgt aacgcagcaa ggaaggcgta tcacaccgcg
    12241 tgctgtgcaa tatcgtgtaa aagaatgggc catcaagcaa ggtgttaaca ctgacatgca
    12301 cccgcatttg ctacgccata gctttgctac ccatgtgctg caatctagcc aagatttacg
    12361 tgctgttcaa gaaatgctcg gccacgccaa cattagcact acgcaggttt acacgcactt
    12421 ggattttcat catctggcca aaatttacga tactgctcac cctcgcgcca agaaaaaata
    12481 aaatacctga ctaatttacc ctgctattat ctcagcgtaa attatggcaa aatagagcca
    12541 cataattttt gactcacaaa aaggaaacat catggaacac accctacccg ctctacctta
    12601 tgcaaaaaac gctttggtgc cacacatttc agaagaaacc tttgaatacc attacggcaa
    12661 acatcaccaa gcctacgtaa caaacttaaa caacttgatt aaaggcacag aatttgaaaa
    12721 tgccagccta gaagacatta tcaaaaaatc ttctggcggt atttacaata actcagcgca
    12781 agtgtggaac cacacattct tttggaacag cttaaaacca aatggcggtg gcgctccaac
    12841 aggtgcattg gctgatgcga ttaacaaaaa atggggttct tttgacgaat tcaaaaaggc
    12901 ttttcaagct tcagccgttg gtaacttcgg ctctggctgg acatggttag tgaaaaaggc
    12961 ggatggttca gttgatattg tgaatatggg tgctgctggc acaccattga caacaggtga
    13021 taaagcgcta ttggcgattg atgtgtggga acacgcttat tacatcgatt accgcaacgc
    13081 acgccctaaa tttgttgaga ccttcttaac ttctttagcc aattgggatt ttgcagccag
    13141 caattttgcc gcttaacatt taaagcgttt taattttagg ctagctttcc ataagctagc
    13201 cttttattct ttaaaagctg cttatagact aaccactatt tttttaaggg atattgaatg
    13261 tctccaaact acgaaacgtt atcggatgct gaaaaatatt tcatcgttat tttattggcg
    13321 ctttatgctg gaacatttct cattggcatc atgttcttag gcgaagtgcc ttgcatgctt
    13381 gatttagagg gtgaatgcaa attcggcaca tactttaagc aattgctgcc attttatggg
    13441 tttacttttg ctctcaacat tgcgattgtg atgtctgctt attacctcaa caagctcgct
    13501 aaaaagtcca tttttatttt tcgcggcctc attttgccac cattgctagc gctgttttat
    13561 tatctttatc actatggtag caattttgtt agggcattat tttagtttat atcgcttagt
    13621 taagctgtta acataagtct atgttgcaac gcatcgtcat tcaatttcta tttatttttc
    13681 tatttgcctt cacgcaaata ggcgttgtta cgcatgaaat cagccatttt tcagatgctg
    13741 ccaagcatac ccagcaagat aaaaacacct cagcagagca atgcgggcaa tgtatcagct
    13801 atgcacaagt cgccagtggc ttgcagccgc acgctttcga gttgcacgac tttataacaa
    13861 gcttcgaact caattcaagc aaacgattta atcttcaacc ctctctttta acggcttacg
    13921 cagcccgcgc cccgcctcaa aacatcagca tttaattttt aaatttctcg gtgctttaat
    13981 caatcgaccg aggtttgcta ttttttgagg ctttcacatg aaaaaacatc acttaaagca
    14041 agtgcgccct gcatttgcgt tgaatacatt atttttggcg cttagttgcc tgtttattga
    14101 aaatgccaag gctgaagcgc ccttgccaac cgacacaacc gcttttgaat tatccaccat
    14161 ctcagtcaca ggcaatccgc tgggcgttgg ttctgacgct ttggtgatac ccatttcaat
    14221 tttaaacgga cgcgaattag caaatcgccg tgaaagcaca cttggcgata cgctcaatgg
    14281 tattccgggt gtggcggcca cgcattttgg gcctaacgca tcacgcccgg tgattcgcgg
    14341 cttagatgct gaacgggtac gtattatgca aaatggcgtt ggtgtgttgg atgcgtctag
    14401 cttgagtttt gaccacgcag tgacaattga cccgctgatt attgagcaaa ttgatgtggt
    14461 gcgcggccct gccgccctgc tgtatggcgg cagtgctgtt ggcggtgtgg tcaatgcaat
    14521 tgaccaccgt attcctaaag agtcgtttga tggtgttact ggccgtgtgg aaggctcaat
    14581 tggcggcgct gaaagccaaa ataatttagc aggcgtggtt gatgtcggtt atggcgcact
    14641 ggtgattcac gctgacgctt acactcgcaa aacaagcgat acggatatag acggctatgc
    14701 tgtatcaagc cgaaaaagcc gtgccgatgg cacaccacga gagaatcgcg gtaagctggt
    14761 caatagtagt gctagtggtg aaggcggtgc attgggcgcg tcgtttattt ttgataacgg
    14821 ttatttaggc gcctcgtact ctgcatttag aaataattac ggtaccgtgg ctgaagaagc
    14881 tgtacgcgca gatatgcgca gtgaccgcgt tgatgtggcg accgagttcc gcgacttagg
    14941 taatgtcatc aaccgcgtaa aagcgcgcat ggcctacact gattatcaac atcaagaaat
    15001 tgaagacggt gaagtaggca ccacctttaa aaatgaaggg gttgaaggta gtttagaatt
    15061 tggccatgca aaaatcggca atttagaagg tgtgctcggc tttcagtttc aaaacacaga
    15121 ctttgaagca ttgggagatg aagcctttgt gccaggggta aatacgcaaa ataaagcact
    15181 ttatctgtat gaagaactcc cgattgatca attaaaactg agctttggcg gacgtgtgga
    15241 gcgtgtcaat gtggattcac gcaacaatgc caaatttggc gcgggtcaaa ataacagttt
    15301 tacgccttac agctatgcgc ttggcgggct ttataccttt aatccaaact ggtctttggc
    15361 gacaaattta tcgcacaacg aacgcgcgcc gagctatttt gagctgtatg ccgatggcgc
    15421 gcacttggcc accgggcaat atgaagtagg taataccaat tttgacaaag agcgctctaa
    15481 tggcatcgat gcacaaatcc gctggaaaga cggtaaaaac tctttcagtg ttggcgctta
    15541 ctacacccgc tttagccgct ttttgggctt gcttgatagc ggcaatacgc gtggtatcga
    15601 tggtgaactg aaccccgatg atttagatgg tgatggcgtt gccgacctat caggagaaga
    15661 cattttacca gagtcgcaat tcagcatgtt tgccgccacc ttcaaaggtt tagaagcaga
    15721 gggaaaattc aacttggtag ataatctgga tttaacccta cgcggcgatt acgtgcgcgc
    15781 aaccaatcgc gataacggcc aagcactgcc gcgcataacg ccgctgcgtt tgggcgctgg
    15841 cttgcggtat caaaaaaatg cactgggtgc cagagttgat
//
LOCUS       DAOQSI010000029        15391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_2919_length_15391_cov_6.174100, whole genome shotgun sequence.
ACCESSION   DAOQSI010000029 DAOQSI010000000
VERSION     DAOQSI010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 15391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15391
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2919_length_15391_cov_6.174100"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PKL42_03335"
     CDS             <1..478
                     /locus_tag="PKL42_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HNU66136.1"
                     /translation="TAPEFYGDVITTRTYQDRLDTLDSVREADINVCCGGIIGMGESR
                     NQRAGLLAQLANMERPPESVPINLLTQVEGTPLHGTEELDPFEFVRTIAIARITMPKS
                     FVRLSAGRQSMHEGIQALCFLAGANSIFYGEKLLTTGNPEAETDKKLFAKLGLHPI"
     gene            482..1642
                     /gene="bioF"
                     /locus_tag="PKL42_03340"
     CDS             482..1642
                     /gene="bioF"
                     /locus_tag="PKL42_03340"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HNU66137.1"
                     /translation="MLDALQKALEQREKDGLLRQRRLLDSPQAEHIVADAKPYLSFCS
                     NDYLGLANHPKLIAAMQQAAGIAGVGSGASGLVTGHHRYHDDLEKQLAKFVNLPAALL
                     FSTGYMANIGVLGALVGRGDAIFADKLNHACLNDGGYYSLAEFNRFAHNDVAALESLL
                     KTSTAKHKLIAVDAVFSMDGDIAPIPDYLALCEKYDVYLYIDDAHGFGVLGEHGAGSL
                     SHFNLQSPRIIMMATLGKAAGVAGAFVAGQQVVIDYLIQTAKSYVYSTPAPPALSATL
                     SASVNIIAQGDALRAHLYALIALLKTELNLTQWQLMPSITSIQPLLIGDNFEAVRVSQ
                     FLQAKGILVPAIRPPTVPKNTARLRISLSAAHSKADVMRLMSALHEAEKEFS"
     gene            1639..2409
                     /gene="bioH"
                     /locus_tag="PKL42_03345"
     CDS             1639..2409
                     /gene="bioH"
                     /locus_tag="PKL42_03345"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HNU66138.1"
                     /translation="MSLHVEIIGEGQPLVMLHGWGSHGGVFHSLVKSMSKSFTLHLVD
                     LPGSGLSRPIDPYHLYSLAEHVADILPAHADVLGWSLGGQVAMRLALDRPDMIRRLVL
                     VGATPCFVNKPDWEIGVPPRVFDDFARDVNLDYQKTMLQNITLQCMNAKDARKTIKII
                     REKFLERPAANTQAIRHGLQVLVDTDLREEVGNLRKPTLLIHGDRDNLAPVQAAHWMM
                     QNLPAGFLRVMSGSAHAPFLSHQAQFIEALEQFLEPAK"
     gene            2409..3308
                     /gene="bioC"
                     /locus_tag="PKL42_03350"
     CDS             2409..3308
                     /gene="bioC"
                     /locus_tag="PKL42_03350"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HNU66139.1"
                     /translation="MLDQYHIDKKRARASFGRAAETYDAAAILQKLVRDEMLSRLDLV
                     NINPHTILDAGCGTGAGSHALQKRFTKAQVVSLDFAMPMLLKTRSPKRQSGLMHQVKS
                     IFGNANSHCLCADIEALPLVTASIGLLWSNLAIQWCNDLDVALQEFHRVLQPEGLLMF
                     STFGPDTLKELRVATAQENTTSVSRFIDMHDIGDALVRAGFSAPVLDVERFTLTYDDV
                     KSVMRDLKSIGAHNATDGRARGLHGRGFLQKIESVYEQFRDGTKSDGGKLPATFEVVY
                     GHAWRGQDKKVLPDGAHVIHFHQ"
     gene            3360..4013
                     /gene="bioD"
                     /locus_tag="PKL42_03355"
     CDS             3360..4013
                     /gene="bioD"
                     /locus_tag="PKL42_03355"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HNU66140.1"
                     /translation="MKQSYFIIGTDTNVGKTYVASLLIQQFVAAGYQTVGMKPIASGC
                     AFDAKGELINDDVQALNVASNVSAPLNLINPYHFSPAIAPHIAAELAGISMQIELIQQ
                     AYTQLTELAEVVIVEGAGGFFVPINATQTLADVAVALNIPVILVVGIKLGCINHALLT
                     VEAIQARGLTLAGWVANEIEPEMQMFEENLSSLTQRIAAPCLGVVYWQKNANFELAN"
     gene            complement(4154..5725)
                     /locus_tag="PKL42_03360"
     CDS             complement(4154..5725)
                     /locus_tag="PKL42_03360"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNU66141.1"
                     /translation="MSNPTVSKKFSIFIIASLALITTACGTNPVTKKREIQLVSESQE
                     IAIGKQNYSPARQSQGGDYIIDPELTAYVQSVGDKLAKVADRQLPYEYAVINDSTPNA
                     WAMPGGKIAFNRGLLYELNSEAELAAVMGHEMVHAAARHGAKSMERGIFMQGAMVAVG
                     IGAQNTNYANLIVGGAQVGAALTMSKYGRDAESESDLYGMQYMKKAGYDPTAAVNLQE
                     TFVRLSADRKSNFIDGLFASHPPSQARVDANKATLAKVGAGGDWGKEVYAQKTAKLKA
                     TQAAYKAYDDGVKALVAKDTAKATALANQAIKGEPREARFQELLGDIALTERKTTEAL
                     AYYDKAIKLQPDYFKPHVQSGIALYNAGKKTEAEPFLKRANELLPTAPGHALLGQIAE
                     ERGDIDNALKHYSVAAGSNSDYGKEANARGVRLDLPRNPSKYLQSGAVADNAGALFAV
                     VQNNTDVPVGRVQVRVVKYDAKTGRAIAQSAPMTISGGVAPGKRNQIAIGEKVATPQE
                     AQLYKVVVEAAEVAK"
     gene            complement(5768..6427)
                     /gene="nadD"
                     /locus_tag="PKL42_03365"
     CDS             complement(5768..6427)
                     /gene="nadD"
                     /locus_tag="PKL42_03365"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HNU66142.1"
                     /translation="MKKYPAIGILGGTFNPIHFGHLRMAEELAEILHLNEVRFIPSAN
                     PPHKPAPAVSAEHRAAMVQLAIANNPTFKFDGRELARTGASYTFDTLSSLREELGAHT
                     SITLFMGNDAFTKLNTWHRWQELLNLCHICLVARAHTASEKSEGLPKALESYLQAHYT
                     ENSEDLLEQAYGCIIMQKMTRLDISSTAIRHALRGKHSVRYLLPDEILSYIHQHQLDY
                     L"
     gene            complement(6461..7717)
                     /locus_tag="PKL42_03370"
     CDS             complement(6461..7717)
                     /locus_tag="PKL42_03370"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNU66143.1"
                     /translation="MNIQTYMQNIGIEARKASRAMASADTLQKNNALNAIAKAILREK
                     DVLLAANKLDLDAARANGLEPAMLDRLAISEKTIASMAEGLTQIANLADPIGEMTDFK
                     YMPSGIQIGKMRVPLGVIGIIYEARPNVTVDAAGLCIKSGNAAILRGGSEAIHCNQAL
                     AKLVHEGLAEAGLPKTAVQVVETTDRAAVGELITMKQYVDVIVPRGGKGLIERISADA
                     RIPVIKHLDGNCHVYVDDDADFDKALRILENSKTQRLGTCNTAESLLVARSVAKEMLP
                     NLADMLTQHGIEIRGCAETCALVKNAIPATDEDYYTEYLAAIISCKVVSGLDEAIAHI
                     NQHSSQHTEAIVTENYTKARRFLREVDSSSVMVNASTRFADGFEYGLGAEIGISTDKL
                     HARGPVGLEGLTSLKYVVLGDGHVRA"
     gene            complement(8560..9597)
                     /gene="holA"
                     /locus_tag="PKL42_03375"
     CDS             complement(8560..9597)
                     /gene="holA"
                     /locus_tag="PKL42_03375"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNU66144.1"
                     /translation="MQLAPAQLEKHLSLGLKSLYVLVGDEPLAQRECLDAIRAAARAQ
                     GLASSGGQIVVERNSLTVERNFNWQQIEAYGQTISLFANLRLLEINIPNGKPGIDGGK
                     ALQALVSKPIPDTTVVIILPKLEREAKNSAWFSALEQHAVLIANDEVSAANLPKWIAN
                     RLGQQGQQASPQTLDFLANQVEGNLLAANQEVQKLGLLYPQGKLTDEAVREAVLNVSR
                     FDAFQLGEAVLLGDAARTARILQGLQDEGENPVAVMSPLMWVLRPLVRVKQAEMRGEN
                     LQTAMTNARIFGDKQALTKRALARLSLRQLEAALQKLADIDKTAKGVMLGDAWLEISR
                     LCFGLAKVRGR"
     gene            complement(9601..10113)
                     /gene="lptE"
                     /locus_tag="PKL42_03380"
     CDS             complement(9601..10113)
                     /gene="lptE"
                     /locus_tag="PKL42_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HNU66145.1"
                     /translation="MKKHVMQYARFNLICLFLLSTTLAACGFQLRGVANLSFKSVFVQ
                     GSVLSISRELNQSFKTNGIQVVNSIEDAELLIEMLNETNEKRILSLSGGGVVREYELN
                     YRVSFRTRDPASETWSAPQTVQARRDFSYNDNALLGKLDEENKLNTDMRSDAVREIMR
                     RLSAIKVTAK"
     gene            complement(10166..12607)
                     /gene="leuS"
                     /locus_tag="PKL42_03385"
     CDS             complement(10166..12607)
                     /gene="leuS"
                     /locus_tag="PKL42_03385"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNU66146.1"
                     /translation="MQQQYPFKEVEESAQTYWETNQTFAASEQSDKPKYYCLSMFPYP
                     SGRLHMGHVRNYTIGDVLSRFHKMKGFNVMQPMGWDAFGLPAENAAIKNNTAPAKWTY
                     ENIEHMKAQLKQLGLGVDWNREIATCKPEYYKWEQWLFTELFKKGLIYKKTSTVNWDP
                     VDGTVLANEQVIDGRGWRSGALVEKRDIPQYFMKITAYAEELLNDLDKLDGWPEQVKT
                     MQRNWIGKSYGCEVEFPIIGQHGNLKVYTTRPDTLMGATYVAVAAEHALATLATQNNP
                     ALAEFVAECKRGSVAEADVATAEKKGMATGLFVTHPLNGEQLPVWVANYVLASYGEGA
                     VMAVPAHDERDFEFAKKYNLPIKTVIKNTNADVLPMTEHGILFNSGAFNTLNFEGAGT
                     AIAEALSAKKLGKRRTQYRLRDWGVSRQRYWGCPIPIIHCEKCGEVPVPAEQLPVVLP
                     EDVVMDGVGSPIKKDPNFYETTCPTCGGRATRETDTLDTFFESSWYFARYASFDCNTA
                     MADSRANYWLPVDQYVGGIEHAILHLLYARFFNKLMRDVGLLKNDEPFTNLLTQGMVL
                     KDGSKMSKSKGNTVDPQALIDTYGADTARLFMMFAAPPEQSLEWADSGVEGANRFLRR
                     LWKAVYDHVELGQIAAYTQGELTAELKAIRLQLHLTIEKVADDYGRRHSFNTAISSVM
                     ELMNALAKIEGTDEVTRSVRQEVLQNVALLLSPIVPHICQAIWAELCPHSNILDAGWP
                     VADKSAMVQDSVEYVIQVNGKLRGSITVAKTENKETLEKLALQQPFVQKFIAENMTVR
                     KVIVVPNKLINVVVS"
     gene            complement(12736..13524)
                     /locus_tag="PKL42_03390"
     CDS             complement(12736..13524)
                     /locus_tag="PKL42_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HNU66147.1"
                     /translation="MRKSDRFVNERPAKDLPVIRRVKPSAVADWLHAGVMDTHRGGWA
                     SLFYGAALTVTGLFIHVFFAKSYWLLAGLTTGFLLLGPFLAIGLYDLSRRMELNEPPK
                     LIPSLTAWLPNLINVSIFAGLLITVMLIWVGISFSIFSHYFNHNLPTFIDVAINVITF
                     KQPTFALIYFAVGGFFAAFIFAISVVAVPLMLDRKASAVSAALASLRVCVRNPLPMLL
                     WAFCIVVLVGFGFATSFLGLIIAMPIVGHASWHVYRDLIETNDK"
     gene            complement(13609..14262)
                     /gene="adk"
                     /locus_tag="PKL42_03395"
     CDS             complement(13609..14262)
                     /gene="adk"
                     /locus_tag="PKL42_03395"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNU66148.1"
                     /translation="MRIILLGAPGAGKGTQATFIKEKFAIPQISTGDMLRAAVKAGTP
                     LGLEAKSFMDSGGLVPDAVIIGLVSERIKEADCANGFLFDGFPRTIPQAEAMKAAGVG
                     IDYVVEIDVPDEAIVERMSGRRSHPASGRTYHVKFNPPKVAGKDDVTGEELVQRDDDK
                     EDVVKNRLDVYHSQTKPLVKYYTDWATSGVAGAPKHVFVNGLGEVTKIRDTIFLALA"
     gene            complement(14504..15268)
                     /gene="rsmA"
                     /locus_tag="PKL42_03400"
     CDS             complement(14504..15268)
                     /gene="rsmA"
                     /locus_tag="PKL42_03400"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNU66149.1"
                     /translation="MKHVAKKRFGQNFLTDQSIITSLVEAISPQPNDLMVEIGPGLGA
                     LTKPLLQKLKLLHVVEVDRDIISWMQQAYAKNNIAIHNSDALKFEFSALGADLRVVGN
                     LPYNISTPILFHLLDNVSAITDMHFMLQKEVVERMVAAPSTPAYGRLSVMLQYHLHME
                     YLFTVPPEAFDPAPKVESAFVRCVPHASLPYVANNTALFAKVVLAAFGQRRKTLRNTL
                     KDWLKDEDFSTLQVDSQLRAENLSVADFVKIANYVS"
     gene            complement(15265..>15391)
                     /locus_tag="PKL42_03405"
     CDS             complement(15265..>15391)
                     /locus_tag="PKL42_03405"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNU66150.1"
                     /translation="MIRTSVDHGTALALAGTGNAEIGSLLAAIKLAIELSQHQHP"
BASE COUNT         3927 a         3629 c         3602 g         4233 t
ORIGIN      
        1 taccgcgcca gaattttacg gtgatgtgat taccacacgc acgtatcaag accgtttaga
       61 tacgttagat agcgtgcgag aagcagatat caatgtgtgt tgcggcggca ttatcggcat
      121 gggtgagtcg cgcaaccagc gcgcgggttt gttggcgcag ttggccaata tggagcgccc
      181 acctgaaagc gtgccaatca atttgctgac acaggtggaa ggcacgccat tgcacggcac
      241 tgaagaactc gacccgtttg aatttgtgcg caccattgcc atcgcccgca tcaccatgcc
      301 aaaaagtttt gtgcggcttt ccgctggccg ccaaagcatg catgaaggca ttcaggcgct
      361 ttgcttttta gcgggggcaa attccatttt ttacggtgaa aaattactca ccacgggtaa
      421 cccagaagcc gaaacggata aaaaattatt tgcgaaatta gggttgcacc cgatttaaac
      481 catgctggat gctttacaaa aagcgttgga acagcgcgaa aaagacggct tattacgcca
      541 gcgacgcttg ctcgattctc cacaagccga gcatattgtg gccgatgcca agccgtattt
      601 atcattttgc agtaatgatt atttaggttt agccaatcac cccaaactca ttgctgcaat
      661 gcaacaggcg gcgggcattg ccggcgtagg tagcggtgca tctgggcttg ttacggggca
      721 tcatcgttac catgatgatt tagaaaaaca gctagcgaag tttgtgaatt tgcccgccgc
      781 tttattattt tcaacaggct acatggccaa tattggggtg cttggcgcgc tggttggccg
      841 tggcgatgcc atttttgccg ataaattaaa ccacgcctgc ttaaacgatg gcggttatta
      901 ttcgttggca gagttcaacc gctttgcgca taacgatgtg gcagcactcg aaagcctgtt
      961 aaaaaccagc actgccaaac ataaactcat cgcagtggat gccgtattta gcatggatgg
     1021 cgatattgcg ccaattccag attatttagc gctttgcgaa aaatacgatg tttacttgta
     1081 tattgacgat gctcacggct ttggtgtgtt gggagaacat ggtgcaggct cgctgagtca
     1141 ttttaacttg caatcaccac gcatcataat gatggcaacg ctcggcaaag ctgccggggt
     1201 agcaggggcg tttgtggcag ggcaacaggt ggtgattgat tatttaattc aaaccgccaa
     1261 aagctatgtc tattcaactc ctgcgccgcc ggcactatcg gccacgctgt cagcttctgt
     1321 caatataatc gcgcagggcg atgcgctacg tgcgcatttg tatgcactga ttgccttatt
     1381 aaaaactgaa cttaacttaa cacaatggca gctcatgccc tcaatcacca gtattcagcc
     1441 gttgttaatt ggcgataatt ttgaagcggt gcgtgtgagc cagtttttac aggccaaagg
     1501 catactggtg ccagccatac gtccgccaac agtgccaaaa aatacggcaa gattacgcat
     1561 ttcgctgtct gccgcacata gcaaagcgga tgtcatgcgt ttaatgagcg cgttgcatga
     1621 agcagaaaag gagttttcgt gagtttacat gttgaaatta ttggagaagg ccaacccctt
     1681 gtgatgctgc acggttgggg ctctcatggc ggtgtttttc actcattagt taaaagcatg
     1741 agcaaaagtt ttaccctgca tttggtcgat ttgcccggct ctggcttaag tcgcccgatt
     1801 gacccgtatc atctgtattc gctcgctgaa catgtggccg atatattgcc agcgcatgcc
     1861 gacgtgctag gctggtcact cggcgggcag gtcgccatgc gcttagcact tgaccgccca
     1921 gacatgatac gcagattggt gctggtaggt gcaacccctt gttttgtcaa taaacccgat
     1981 tgggaaatcg gcgtgccacc gcgtgtgttt gatgattttg cgcgggacgt aaacttggat
     2041 taccaaaaaa ccatgttgca aaacattacc ctgcagtgca tgaatgcgaa agatgcgcgt
     2101 aaaaccatta aaattattcg tgagaaattt ttagaacgcc cagccgccaa cacgcaggca
     2161 atccgtcatg gcttgcaagt gctggtggat actgatttac gcgaagaggt tggtaattta
     2221 agaaagccaa cactgttgat tcatggcgac cgcgacaatt tggcccccgt acaagccgcc
     2281 cattggatga tgcaaaattt gcccgctggc tttttacgcg tcatgtcggg ctcagcacat
     2341 gcaccatttt tatcacatca agcacaattt attgaagcgc ttgaacagtt tttagagcca
     2401 gccaagtaat gttggatcaa taccatattg ataaaaagcg cgcgcgcgct tcatttgggc
     2461 gcgcagctga aacttacgac gcggccgcca ttttgcaaaa gctggtacgc gacgaaatgc
     2521 tcagtcgtct ggatttagtt aacattaacc cacacactat attagacgcg ggctgtggca
     2581 ctggggcggg cagccacgcc ttgcaaaaac gttttacaaa agcacaagtg gtgtcactgg
     2641 attttgcaat gcccatgctg ctaaaaacgc gcagcccaaa gcgtcaatca ggcttaatgc
     2701 atcaggtaaa gtcaattttt ggcaacgcaa actcccattg tttatgcgcg gatattgaag
     2761 ccctgccttt ggtaacggcg agtattggtt tgctgtggtc aaacctggcc attcagtggt
     2821 gcaacgattt agatgtagcc ttgcaagaat ttcaccgcgt tttgcagcca gaaggcttgc
     2881 tcatgttcag cacctttggg ccagatacgc tgaaagaatt acgtgtggcc accgcgcaag
     2941 aaaacaccac cagcgtgagt agatttattg acatgcacga cattggcgat gcgctggtgc
     3001 gggcaggttt tagcgcacct gtgttagatg ttgaacgctt tacgctcact tatgatgatg
     3061 tgaaaagtgt gatgcgcgac ttaaaaagca tcggcgcgca taacgcaacc gatggccgtg
     3121 cgcgcggcct gcacgggcgt ggatttttgc aaaaaattga atcagtgtat gagcaattca
     3181 gagatggcac aaaatcggat ggcggaaaat tgcccgcgac ttttgaagtg gtttatgggc
     3241 atgcctggcg cggacaagat aaaaaagtgc tgccagatgg tgcgcatgtg attcattttc
     3301 atcaataaat caccgtcatt cccgcgcaga aatgacgatt aaggaagctg caagcttaaa
     3361 tgaaacaatc ctacttcatc atcggcaccg ataccaacgt gggcaaaacc tatgttgcga
     3421 gcctactgat tcagcagttt gttgctgctg gctaccaaac ggttggcatg aagccaatcg
     3481 catcaggttg tgcgtttgat gcaaaaggtg aactcatcaa tgacgatgtg caagcactca
     3541 acgtcgccag caatgtcagc gcaccactca atttaatcaa cccttatcac ttttcgcccg
     3601 ccattgcgcc ccacattgcc gcagagcttg ctggtattag tatgcaaatt gaattgattc
     3661 aacaagctta cacacaactt accgagttgg cagaagtggt gatagtagaa ggtgcgggcg
     3721 gtttttttgt gccaataaac gcaacgcaaa cgctggcaga tgttgctgta gcattaaata
     3781 ttcccgttat tttagtggtc ggtatcaagc ttggttgcat caaccatgcc ttactcacgg
     3841 tagaagccat acaagcgcgt ggtttaactt tagctggttg ggtcgctaat gaaattgaac
     3901 ccgaaatgca gatgtttgaa gagaatttaa gcagcctcac acagcgtatt gcagcgccat
     3961 gtttgggggt ggtgtattgg caaaaaaacg ccaattttga actagcaaat taggcgcatt
     4021 caaagtctga attcgtacga gaaaaaaata atgccctgta agcctttatt tataaggctc
     4081 gcagggcatc gaaaaaggtt ggtgttaaaa ttgcctaatt tttaagcatt tttgcataca
     4141 taatatgcgc tgattattta gctacttctg ccgcctcaac caccacttta taaagctgcg
     4201 cctcttgcgg tgtggcgact ttttcgccaa tggcaatttg gttgcgttta ccaggagcta
     4261 cgccaccaga aattgtcatc ggcgcacttt gtgcaatggc gcggcctgtt tttgcgtcat
     4321 atttcaccac acgcacttgc acgcgcccca cgggtacatc agtattattt tgcactacgg
     4381 caaataaagc gccagcgttg tcggccactg cgccagattg caagtatttg ctcgggttac
     4441 ggggtaagtc taagcgcacg ccgcgcgcat tggcttcttt cccatagtct gaattagaac
     4501 ccgcagcaac agagtaatgt ttcagcgcat tatcaatgtc accccgttct tcagcgattt
     4561 gcccaagtaa tgcgtggccc ggggcggtag gaagcagttc attcgcgcgt tttaaaaaag
     4621 gctcagcctc agtttttttg cctgcattat ataaagcaat gccgctttgc acatgcggtt
     4681 taaagtaatc aggttgcaat ttaatggctt tatcataata ggccagtgct tcagttgttt
     4741 ttctctcggt gagcgcgatg tcgcctaata gttcttgaaa gcgcgcttca cgcggttcac
     4801 ccttaatcgc ttggttggca agcgcggtgg cttttgcggt atcttttgct accaatgctt
     4861 tcacgccatc gtcataggct ttataggccg cttgcgtggc ttttaacttg gcggtttttt
     4921 gcgcatacac ttctttaccc caatcgccac ctgcgcctac tttagccagc gttgctttat
     4981 tggcatccac ccgtgcttgt gaaggcgggt ggctggcaaa taagccatca ataaagttgc
     5041 ttttgcgatc agcactcagc cgcacaaacg tttcttgtaa attcaccgct gccgttgggt
     5101 cgtagcccgc ttttttcatg tactgcatgc cgtataagtc agattcactt tcagcatcgc
     5161 ggccatattt gctcatggtg agcgcagcac caacttgcgc gccaccaact attaaattag
     5221 cgtaattggt gttttgcgca ccaatgccaa cggcaaccat cgcgccttgc ataaatatgc
     5281 cgcgctccat gctttttgcg ccatgccttg ctgctgcgtg caccatttca tggcccatca
     5341 ccgccgccag ttcagcctca ctatttaatt catacagtaa gccacggtta aaggcaattt
     5401 tgccacctgg catcgcccac gcgttggggg tagagtcgtt aattacggca tattcataag
     5461 gcagctggcg gtcagccact tttgcgagtt tatcgcccac actttgcaca tacgcagtca
     5521 attcagggtc gataatataa tcgccgcctt gcgactgccg cgcgggcgag taattttgct
     5581 taccaatggc aatttcttgc gattctgata ccagctgaat ttcacgcttt ttagtcacgg
     5641 ggtttgtgcc gcaggcggtg gtaatgagcg ccaagcttgc gataatgaaa atagaaaatt
     5701 ttttagaaac ggtagggttt gacatggttc actctcttac tgcacaaaaa gttaggtaaa
     5761 aaaacagtta caaatagtca agttgatgct gatggatata gctcaatatc tcatcaggca
     5821 ataaataacg cacagaatgt ttgccacgca atgcatgacg tatggctgtg gatgaaatat
     5881 ccagccgcgt cattttttgc atgataatgc atccgtaagc ctgttcaagc aaatcttcac
     5941 tgttttcagt gtaatgcgct tgcaagtagc tttcaagtgc cttgggcaag ccctcagact
     6001 tttctgaagc ggtgtgcgcc cttgccacca agcaaatgtg gcaaaggttc aaaagctcct
     6061 gccagcgatg ccaagtgtta agttttgtaa aagcatcatt tcccataaaa agtgtgatgc
     6121 tggtgtgcgc gccaagttct tctcttaaac tcgataacgt gtcgaatgta taagatgcac
     6181 ccgtgcgcgc aagttcacga ccatcaaact taaaggtagg gttgttggca atggccaatt
     6241 gcaccatcgc cgcccgatgc tcggcactta ccgcaggggc aggtttgtgc ggcgggttgg
     6301 cggagggaat aaaacgcact tcgtttaagt ggaggatttc agcgagttct tcagccatac
     6361 gcaaatgacc aaagtgaatc gggttaaacg tgccaccgag aatgccaatt gcagggtatt
     6421 ttttcattgg attatctcag gcctctaagg gctagtttag ctatgcacgc acatggccat
     6481 cgcccaacac cacatacttg agtgaagtca ggccttctaa acccacaggg ccgcgagcgt
     6541 gtaatttatc ggttgaaatg ccaatttcag cacccaagcc atattcaaaa ccatcagcaa
     6601 agcgggttga ggcattgacc atcacagaac ttgaatccac ctcgcgcaaa aaacgacgtg
     6661 ccttggtgta gttctcagtc acaatcgctt cggtgtgttg gctagaatgt tggttaatat
     6721 gcgcaatcgc ctcatctaaa ccacttacca ctttgcatga aataatcgcc gctaagtatt
     6781 cggtgtaata atcttcatcg gtggctggaa ttgcattttt tacaagcgcg caagtttctg
     6841 cacagccgcg aatttcaatg ccgtgttggg tgagcatatc tgccaaattt ggcaacattt
     6901 ctttggctac agagcgtgca accagtaatg attccgccgt gttgcacgtg cctaagcgct
     6961 gcgttttact gttctctaaa atccgcaacg ctttgtcaaa atccgcatcg tcatccacat
     7021 acacgtggca attaccatct aaatgtttaa tcacaggaat gcgcgcatcg gcactaatgc
     7081 gttcaatcaa acctttgccg ccgcgtggca caatcacatc cacatattgc ttcatggtaa
     7141 tcagctcgcc caccgccgcg cggtctgtcg tttcaaccac ttgcacggca gtttttggca
     7201 ggccagcctc cgccaagcct tcatgcacca gtttagccaa agcttggtta cagtgtatag
     7261 cttcgctacc gccacgcaaa atggccgcat tgccagattt aatgcacagc ccagcggcgt
     7321 ctaccgtcac attcgggcgt gcttcataaa taatgccaat tacgcccagc gggacgcgca
     7381 ttttgccaat ttgtataccg ctcggcatat atttaaaatc agtcatctcg ccgattgggt
     7441 cagcaagatt agcgatttgt gttaaacctt ccgccatgct ggcaatggtt ttttcgctaa
     7501 tcgctaaacg gtctagcatc gctggttcta agccgtttgc acgcgcagcg tctaaatcga
     7561 gcttattggc cgccagcaat acgtcttttt cacgcaaaat cgccttggca attgcattca
     7621 atgcattgtt tttttgaagt gtatcggcac tcgccattgc gcgtgaagcc ttgcgggctt
     7681 caatgccaat gttttgcata taggtttgaa tgttcatgct gtgattatac ttttttgagg
     7741 gatttttaac agtaaatttg gaggccgaat ttgagcaatt aaatcgtaaa aataacacaa
     7801 aaaatccctc gagagatttt tatgcctctg gaatcctcat ggatacttgc ttgcagagga
     7861 ggtgaaatga tttggtgtga atttagtgat gaaaaatggg ttttgtaggg cggatgagcg
     7921 cagtgttatc cgccgtaagg gtattacatt cggcggaagg cgtttcactt ttccgcccta
     7981 cgagctaagt ctttgataat gttatttagt tggcttcagt gttcttcgta ttgatttcta
     8041 aaagcttact tgttaataat ccaattgcaa tgttactagt gccgctaatt aataaggata
     8101 tatagccatc tactaacaaa atgaatcttt gcaaaattcc aaaatcacta gctaacgcat
     8161 gctgcaattt cagggcttca taaatttcta ctttaagttc atttccaaat taagtaagat
     8221 ttttgataaa tatttcatgt tcaactactc ctacatcata aaagtggata ttttcttctt
     8281 cctcgagagt tagcagcata tctttgaaaa cgctgataaa gttatccagt ctatggtcat
     8341 agtttttatg aagatattca cttacatcgt caagtagctg tttttttgag cttctaactt
     8401 ggttttattg tttaaaatta atattgcaat aacaatgagt gcagctatca atagacctaa
     8461 gagcattcca acctccttaa ccaaaatatt gtttttgcgt attactataa ataaaaatcc
     8521 catgtaacag gtgtatgcaa aaagtaatac accttaaagc taacgccccc gcaccttcgc
     8581 caacccaaag caaagccgcg aaatttctag ccaggcatca cctagcatta cgcctttagc
     8641 ggttttatca atatcggcca gtttttgtaa ggcggcttca agctgacgca ggcttaatct
     8701 tgccaatgcg cgtttggtga gggcttgttt gtcgccaaaa atgcgggcgt tggtcatggc
     8761 ggtttgtagg ttctcgccac gcatttcggc ttgttttacg cgcacaagtg gtctcagcac
     8821 ccacatgagc gggctcatca cggcgactgg gttttcgcct tcatcttgta agccttgtaa
     8881 aatacgggca gtgcgggcgg catcgcccaa tagcacggct tcaccgagtt gaaaagcatc
     8941 gaagcgcgaa acatttagca cggcttcgcg cacagcctca tcggtgagtt taccttgtgg
     9001 gtaaagtaag ccgagttttt gcacttcttg attggcggcc agtaggttgc cttctacttg
     9061 gttggctaaa aaatcgagcg tttgcgggct ggcttgttgg ccttgctggc caagcctgtt
     9121 tgcaatccat ttaggtaaat tggctgcgct gacttcgtcg ttagcaatca ataccgcgtg
     9181 ttgctcaagc gcactaaacc atgcgctgtt tttggcttcg cgctcgagtt ttggcaaaat
     9241 aatcaccaca gtggtgtcag gtataggttt gctcactaag gcttgcagcg ctttaccacc
     9301 gtcgatgcct ggcttgccat tgggaatgtt gatttcgagt aagcgtaagt tggcaaatag
     9361 tgaaatagtt tggccgtaag cttcaatttg ttgccaatta aaattgcgtt caacggttaa
     9421 gctatttcgc tcaactacta tttgcccgcc gctagaggcg agaccttgcg cgcgggctgc
     9481 tgcgcgaatc gcatctaggc attcgcgttg tgcaagcggc tcgtcaccca ccaacacata
     9541 taaacttttt agcccaaggc ttaagtgttt ttcaagttgc gcaggcgcaa gttgcatggc
     9601 ttatttagcg gtgactttaa tggctgataa tcggcgcata atttcacgca cggcgtcact
     9661 acgcatgtca gtattaagtt tgttttcttc atccaacttg ccgagtaggg cgttgtcatt
     9721 atatgaaaag tcacggcgtg cttgcacggt ttgcggagcg ctccaggttt cacttgcggg
     9781 gtcacgcgtt ctaaaactga cgcgataatt aagttcgtat tcacgcacca caccgccacc
     9841 actgagtgac aaaatgcgct tttcattggt ttcgttgagc atttcaatga gcagctctgc
     9901 atcttcaata ctgttcacca cttgaatgcc attggttttg aaagactggt taagctcacg
     9961 agaaatgctt aaaacgctgc cctgcacaaa cactgattta aaagacaaat ttgccacgcc
    10021 gcgcagttga aacccgcagg cagcaagtgt cgtactcaat aaaaataagc agattagatt
    10081 aaagcgtgca tattgcatga catgtttttt catcggtttt cctgctcgta gggttagcct
    10141 gctttaagtt tagctgactt taagtttagc ttactacaac gttaataagt ttattgggta
    10201 ccacaatgac tttacgaaca gtcatatttt ctgcaatgaa tttttgcaca aaaggctgtt
    10261 gcaaggctaa cttttcaagc gtttctttgt tttctgtttt ggcaacggtg atactgccac
    10321 gcaatttacc gttgacctga atcacatact ccacactgtc ttgcaccatg gctgatttgt
    10381 cggcgactgg ccagccagca tcgagaatat tgctgtgtgg gcatagttca gcccaaatgg
    10441 cctggcagat gtgcggcaca atcggcgaga gtagtagtgc tacattttgc aaaacttctt
    10501 ggcgaacact gcgtgtcact tcatctgtac cttcaatttt tgccagcgca ttcatgagtt
    10561 ccatgaccga tgaaatggcc gtgttaaaac tatggcgacg gccgtagtca tcagcgactt
    10621 tttcaatagt gagatgtaat tgtaagcgaa tcgccttgag ttcggccgtc agctcacctt
    10681 gagtataggc tgcaatctgc cctaattcta catggtcgta tacggctttc cagagtctgc
    10741 gtaaaaagcg gttggcacct tcaacgccac tatccgccca ttccaagctt tgctcaggtg
    10801 gggcagcaaa catcataaaa agccttgctg tatcagcacc ataggtatca atcagtgctt
    10861 gcgggtcaac cgtattgcct tttgatttgc tcatttttga gccatctttc agcaccatgc
    10921 cttgggtgag caagttggta aatggttcgt catttttcaa caacccgaca tcgcgcatca
    10981 atttgttaaa aaagcgcgca tagagtaagt gtaaaatagc atgctcaatg ccgcctacat
    11041 attgatccac aggtagccag tagtttgcgc gcgagtctgc catggcggtg ttgcagtcaa
    11101 agctcgcgta acgcgcaaaa taccatgaag actcgaaaaa tgtgtctaac gtatcggttt
    11161 cacgcgtggc tctgccgcca caagtggggc aagtggtttc ataaaagttt gggtcttttt
    11221 taattgggga gccaacgccg tccatcacca catcttcagg caataccact ggtagttgtt
    11281 ctgcaggcac gggtacttca ccacattttt cacagtgaat aatgggaatt gggcaacccc
    11341 aataacgttg acgcgaaaca ccccaatcgc gcagacgata ttgggtgcgg cgtttaccga
    11401 gctttttggc agaaagcgcc tcggcaatcg ccgtaccagc gccttcaaaa ttaagcgtat
    11461 taaaagcacc agaattaaac aaaatgccat gttccgtcat gggtaataca tcagcattgg
    11521 tatttttgat caccgtttta atgggcaagt tatatttttt tgcaaactca aaatcgcgtt
    11581 catcgtgtgc tggtaccgcc ataacggcgc cttcaccata gcttgccaat acgtagttag
    11641 ccacccacac aggcagttgc tcgccattta aaggatgggt gacaaataag ccggttgcca
    11701 tgcctttttt ctcagccgtc gctacatcgg cttcagccac actaccgcgc ttacattcgg
    11761 caacaaactc agccagtgca gggttgtttt gtgtagcaag ggttgctaaa gcgtgttctg
    11821 ccgcaactgc cacataagtc gcgcccatga gcgtatcggg gcgtgtggtg tacactttta
    11881 agttgccatg ttgaccaata atcggaaact ccacttcaca gccgtagctt ttacctatcc
    11941 agttgcgctg catggttttt acttgctctg gccagccatc gagtttatct aaatcgttca
    12001 ataactcttc agcgtaagcc gtgattttca taaagtattg tggaatatcg cgcttttcaa
    12061 ccagtgcgcc actgcgccaa ccacggccgt caatcacttg ctcattagca agcacagtgc
    12121 cgtccactgg atcccaattt acggtggatg tttttttgta gattaggcct tttttaaaaa
    12181 gctcggtaaa aagccattgt tcccacttgt aatactctgg cttgcaggta gcaatttcgc
    12241 ggttccagtc caccccaagc cccaactgct tgagttgggc tttcatgtgt tcaatatttt
    12301 catacgtcca tttggcaggc gcggtgttgt ttttaatcgc cgcattttcg gcgggcaagc
    12361 caaacgcatc ccaacccatc ggctgcatga cgttaaagcc tttcatttta tgaaaacggc
    12421 tgagaacgtc accaatggtg taattgcgca cgtgccccat gtgcaaacgg ccacttgggt
    12481 agggaaacat cgataaacaa tagtattttg gtttatctga ttgttctgag gctgcgaaag
    12541 tttggttcgt ttcccagtag gtttgggcag attcttcgac ttctttgaaa ggatattgct
    12601 gttgcatggt taagctattc ttgaaatttt ttggacggct taattatacc gtgaagcggt
    12661 gattgcgtca tggtttgtca cgcagcgtaa tgctttgtca gcccacaact ttcctgcatc
    12721 acatgccagc aatgcttact tgtcattcgt ttcgataagg tcacgataga cgtgccaact
    12781 tgcatgccca acgattggca ttgcaataat taaacctaga aagctcgtgg caaaaccaaa
    12841 gccgacaagg acaacaatac aaaaagccca aagaagcata ggtaatgggt tgcggacgca
    12901 gacgcgcagg ctggccaatg cggcactgac tgcactggcc ttgcgatcta gcatcagcgg
    12961 tacggcaaca acgctaatgg caaagataaa ggctgcaaaa aatcctccaa cagcaaaata
    13021 aatgagcgca aaagtgggtt gtttaaaagt gattacattg attgcaacat caatgaaagt
    13081 ggggagatta tgattaaaat aatgcgagaa aatgctaaat gagattccaa cccagatgag
    13141 catgactgta ataagcagcc ctgcaaaaat gctcacgtta attaaattcg gcagccatgc
    13201 agttaggctt ggtataagtt tcgggggttc gttaagctcc atgcgccgac ttaggtcgta
    13261 tagtcctata gccagaaatg gaccgagcag cagaaatcca gtggttaagc cagctaatag
    13321 ccaatagctt ttagcaaaaa aaacatgaat aaacaagccc gttaccgtaa gcgctgcgcc
    13381 atagaaaagg cttgcccagc cgcctcgatg cgtgtccatt acgcctgcat ggagccagtc
    13441 ggcgactgca ctcggcttga ctcgccgaat gacaggtaaa tccttggcag ggcgctcatt
    13501 gacaaaacga tcacttttac gcattaacaa cccctttgca gttttaatga caacgcactt
    13561 cagctaattt gaactgacat acgcatggtg ctgactacaa cttaaagctt aagcaagtgc
    13621 cagaaaaata gtatcccgaa tttttgtcac ttctcccaac ccattcacaa acacatgttt
    13681 tggtgcacct gcaacgccac tcgttgccca gtctgtgtaa tatttgacta acggtttggt
    13741 ttggctgtga tacacatcga ggcgattttt aacgacatct tctttatcat cgtcgcgctg
    13801 aacaagttct tcacccgtta cgtcgtcttt gcccgccact tttggaggat taaatttaac
    13861 gtgataagta cgacctgagg cagggtggct acggcggccg ctcatgcgct caacaatcgc
    13921 ttcatctggc acgtcgattt caaccacata atcaatgcca acaccggccg ctttcatggc
    13981 ttcagcctgc ggaattgtgc gaggaaagcc gtcgaataga aagccgtttg cgcagtctgc
    14041 ttctttaatg cgctcactta ccaagccaat aatcacggca tctggcacca atccgccgct
    14101 atccataaag cttttggctt ctaagcccag cggtgtgcca gctttcacgg ctgcgcgtaa
    14161 catgtcgcca gtggaaattt gcggaatggc aaacttttct ttaatgaacg ttgcctgtgt
    14221 gcctttgcct gcgcccggtg cgccaagaag aatgattcgc atgttgaagt cctaaagagg
    14281 gttgatttga gtttattttg aaaataatta tagcagaaga cgcgtgcgtc aattttgtaa
    14341 aatattgcgc caaaaacagt atgattttta atgtaaatac cacatgttta atacacaacc
    14401 tttttggatg ctctggaagc ctttatttac aaggctcgca gagcataaaa tttttctcga
    14461 ggggttttaa gcgatttttt aatgttttta cagcacttaa tttttagctt acataattgg
    14521 ctattttgac aaaatcagcc acgcttaaat tttctgcacg cagttgcgaa tcaacttgca
    14581 atgttgaaaa gtcttcgtct tttaaccagt ctttgagtgt gttgcgcagg gttttacggc
    14641 gttgcccaaa tgccgccagt acgacttttg caaacagggc tgtgttgttg gcaacgtagg
    14701 ggagactggc atgtggcacg cagcgcacaa aggccgactc aactttaggt gcaggatcaa
    14761 aggcctcggg tggcacggtg aagagatatt ccatgtgcaa atggtattgc aacatcacag
    14821 aaagccgccc gtaagcaggc gtggaagggg ctgccaccat gcgttcaacc acttcttttt
    14881 gcaacataaa atgcatatca gtaatcgcac ttacgttatc gagcaggtga aaaagtatgg
    14941 gtgtggaaat gttataaggt agattgccga ccacgcgcaa gtctgcgcca agtgcgctaa
    15001 attcaaactt tagcgcgtcc gaattatgaa tagcgatatt gttttttgca taggcttgct
    15061 gcatccaact gatgatgtca cggtctacct ccaccacgtg caaaagcttt aatttttgta
    15121 gcagcggctt agtcaacgcg cccaagcccg ggccaatttc aaccattaaa tcattaggtt
    15181 gcggtgaaat agcttcaacc aagctggtga taatgctttg gtcggtaaga aaattttgcc
    15241 caaagcgttt tttggcgacg tgtttcatgg gtgttggtgc tgactaagtt caatggccaa
    15301 tttaatggcg gccagcaagc tgccaatttc ggcgttgccg gtgcctgcta gcgccaacgc
    15361 ggtgccatgg tcaaccgagg tgcgaatcat t
//
LOCUS       DAOQSI010000030        15053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_3019_length_15053_cov_4.729097, whole genome shotgun sequence.
ACCESSION   DAOQSI010000030 DAOQSI010000000
VERSION     DAOQSI010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 15053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15053
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3019_length_15053_cov_4.729097"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..755)
                     /locus_tag="PKL42_03410"
     CDS             complement(93..755)
                     /locus_tag="PKL42_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNU66151.1"
                     /translation="MIQSSLEWLSLFPELAQLDQHAKEILAKYARIVEAPIGTIGYRE
                     GMPCGAYVMRLAGKSRVYKMSTSGREILLYRVGAGETCVITTTCLLGDSDYPASTIVE
                     EPIRDVLIPANAFHQLMIDSAVFRKFVMTNYGALISDLIVLLDEVAFHSLNARLAKVL
                     LDANTTTITRTHQKIADELGTAREVVSRQLKRLEQKGAVSLGRGHVEITDRKTLEKMA
                     NV"
     gene            complement(868..1572)
                     /locus_tag="PKL42_03415"
     CDS             complement(868..1572)
                     /locus_tag="PKL42_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNU66152.1"
                     /translation="MLFKQLFDTDSSTYTYFIADIDAGEAVLIDPVNTHIDEYLHLLN
                     THKCSLKYSLETHVHADHITGGGKLRQKTDALTAVSAACGAQTADLQLNDGDVISFAN
                     ETITVIATPGHTLGSISFLWRDRLFTGDALLINGCGRTDFQGGSAESQYNSIVNKLFS
                     LPDETLVYPGHDYQGRRVSCIGQEKVINPRLAGKSLDEFVKIMNNLNLPKPRLIDIAV
                     PANRMCGVPENTMMQG"
     gene            1692..2006
                     /locus_tag="PKL42_03420"
     CDS             1692..2006
                     /locus_tag="PKL42_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNU66153.1"
                     /translation="MIDIKDINFDELRESADKASHLMRVMANADRLMLLCQLSQGEKS
                     VGELQDLLKIYQPTLSQQLTVLREAELVATRREGKSIIYRISSQPALAVMQVLSQQIC
                     HI"
     gene            2055..2399
                     /locus_tag="PKL42_03425"
     CDS             2055..2399
                     /locus_tag="PKL42_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HNU66154.1"
                     /translation="MTIEINEINQQFSTAPQLSTEDFAEIAKLGYKTIINNRPDYEGG
                     DAQPNSASLKAAAEAAGLAYFYIPVIPNNIQADEVAAFAQTFSVATKPVLGFCRTGNR
                     AGKLFQLAQVSA"
     gene            2438..3262
                     /locus_tag="PKL42_03430"
     CDS             2438..3262
                     /locus_tag="PKL42_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNU66155.1"
                     /translation="MVDPQIISILLGLFVGLVLALTGAGGSVIAVPLLAFFLNLSILQ
                     AAPIGLIAVTFAATIGALHGLQAGIVRYKAAALMAAFGVLLAPAGVWFAHRIPNQWLH
                     LVFSVVLIAVAWRIWKQDLTTPTIAEENPAFACAVNPATSKLFWTASCTKRLITVGSL
                     SGFLSGLLGVGGGFVIVPTLRKFSNLALNTIVATSLAIIALVSAVSVASYMLHGQINW
                     QIAMPFMLATATGMLAGRTLAGKLSSQFTQRSFAILAWLIAAMLIYQWVNITLFGS"
     gene            complement(3387..3947)
                     /locus_tag="PKL42_03435"
     CDS             complement(3387..3947)
                     /locus_tag="PKL42_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66156.1"
                     /translation="MQKKWLRICLVFSLALCLLGCSKTEQLSAQEQSLLLTELDFAAF
                     ELAIEKAPAGVFLKEVNYLAHATDYTYEVTTKDSFYLHSTFSDEQSAIDAKITATAMR
                     TGALIGLTVGEMKNQEIVLKNQFGGNAKLSLLLMHGKPAGNLFTFAHGNKSASVIFTG
                     VYFDNEADFSDFFTPYITHIKIAKLH"
     gene            complement(3953..5407)
                     /locus_tag="PKL42_03440"
     CDS             complement(3953..5407)
                     /locus_tag="PKL42_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HNU66157.1"
                     /translation="MSLILVQNAALGSENSLSSENHILLVMDGDAKDAFPYAKLLETK
                     LKRSKSALKDLNKTPVTIELPHGGIASVVVLDNSLNVFQKHTLLRKAIKPLIAEQPEQ
                     LAICVFGSEAARKANACAAYYVAAVNAAELPSRKKEHQHEPLKAIHIYGWQEDHNYAF
                     TNARVAGNTLCRQLTRLPPNELTPTLYREKIKTLASEYGWDWEAFDMPTLKTMGAGAF
                     YAVGQGSEPQDAAIVHLTYQPEKATKHIALVGKGICFDTGGHNLKPAKYMHGMHEDMN
                     GSAVALGILAAATLQKLPVKLDCWLAIAQNHISPAAYKQNDVVKALNGMTIEIIHTDA
                     EGRMVLADTLTLASRKKPDAMLDFATLTGSMQTALADRMSGIIANRPELACMAVGAGA
                     AAGERIVAFPYDDDYDSDLDSDIADIKQCTLEGGGDHIHAARFLGKFIENDIAWLHMD
                     LSSYNRKDGLGAVQSDINGFGVGFGLEMLKRLME"
     gene            5622..8291
                     /gene="aceE"
                     /locus_tag="PKL42_03445"
     CDS             5622..8291
                     /gene="aceE"
                     /locus_tag="PKL42_03445"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HNU66158.1"
                     /translation="MTFNQQTGLQETDAQETQEWLDALASVIETEGTERAHFLLEAMI
                     DQARRSGSNLPYNATTAYVNTIPTHLQAKLPGDPEMERRVRALVRWNAIMTVLRANEK
                     SPGVGGHIASFQSAATLYDTGFNYFFRAPNENFGGDCVYFQGHSSPGVYARAFLEGRF
                     TEEQMDNFRQETGGNGLSSYPHPWLMPDFWQFPTVSMGLGPLAGIYQARFLKYLHDRG
                     IADTSDRKVWVFCGDGEMDEPESQGAISLASREKLDNLIFVINCNLQRLDGPVRGNGK
                     IIQELESNFRGSGWNVLKVIWGSYWDPLLAMDKDGLLKKRMEECVDGEYQNFKAKGGA
                     YTREHFFGKYPQLKEMVAAMSDSDIWRLNRGGHDPHKVYAAYNAAVNHKGQPTVILAK
                     TVKGYGMGNAGEAQNTTHQQKSMDIESLKKFRDRFDLPITDEQVENLSFYKPAEDSPE
                     MQYMAARRAAMGGFVPQRRRKGNALTVPELSAFENMLAATGEREISTTMAFVRILSTL
                     VRDKQIGKYVVPIVPDEARTFGMEGLFRQLGIYSSAGQLYEPMDSDQVMFYKESKDGQ
                     ILEEGINEAGSFASWLAAATSYSVTGTQMIPFYIYYSMFGFQRIGDLAWMAGDSRARG
                     FLLGATAGRTTLNGEGLQHEDGHSHLMSAMIPNCVSYDPTFAYELAVIIQEGLRRMVQ
                     NQEDVYYYITLMNENYSHPALPKGAEEGILKGLYSFSKSKAKGEKVQLMGSGVILREV
                     IAAAELLESDWGVSADVWSATSFTELRREGLDCERWNLLNPEKPQKVNFVAQSLKDAK
                     GPVIASTDYMKSFAEQIQRFVPNKFVALGTDGYGRSDSREALRDFFEVNRYYVTVAAL
                     KALSDEGKLPVSKVAEAVKKYKLNAKKPSPTSI"
     gene            8315..9658
                     /gene="aceF"
                     /locus_tag="PKL42_03450"
     CDS             8315..9658
                     /gene="aceF"
                     /locus_tag="PKL42_03450"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HNU66159.1"
                     /translation="MALKDVLVPDIGNFDSVDVIEVLIKTGDTIAKDDSLITVESDKA
                     SMDIPAPFAGVVKEVKVKIGDKVAEGTLILTLEASDVATATVEVKPASVPEVPEVPKA
                     AAPVAAIPEPSRPAPEPPKQVQPAQQPAPVGEHVVVAAGKLSHASPSIRKFAREIGVN
                     LALVKGSGPKNRILQSDVQSFVKGELAKPRTENMGAGLGALATLPMPVIDFSQFGAIE
                     TKPLSRIKKLSGANLHRNWVTAPHVTQFDEADITDLEDFRKSMQADAEKRGVKLTMLA
                     FLIKASVNALKAYPNFNASLSPDGDSLILKNYYNIGFACDTPDGLVVPVVKDVQSKDV
                     LDIARDLMELSTKARERKLKVEEMQGGTFTISSLGGIGGTMFTPIINCPEVAILGVSR
                     SSMQPVYDKTTNGFEPRLILPLSLSYDHRVVDGADGARFTSHMRMMLSDVRRLLL"
     gene            9695..11485
                     /gene="lpdA"
                     /locus_tag="PKL42_03455"
     CDS             9695..11485
                     /gene="lpdA"
                     /locus_tag="PKL42_03455"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNU66160.1"
                     /translation="MIEIKVPDIGNFDSVDVIDVLVKVGDTIAKEDSLITVESDKASM
                     DIPAPQAGVVKELKIKVGDKVAKDSLILLLETDGVKNDAPEALQNKASSPVAEKVAEK
                     AVENPVTAPIPAVATSGNDISTEVVVLGSGPGGYTAAFRAADLGKKVVLIERYATLGG
                     VCLNVGCIPSKALLHTAKVITEAQETAHHGVSFGAPKVDLDTLRNWKANDVVGKLTGG
                     LAAMAKGRGVTVVQGVGKFTSPNQIAVTLNDGSAADGKVTTIGFEHAIIAAGSQATKF
                     PGAPDDERIMDSTGALALADIPKRMLVIGGGIIGLEMGTVYDALGTKVSVVEFMDGLI
                     TGCDRDLVRPLQKRMEKRFESIMLSTKVSKIEAKKDGIHVSFEGENAPDGVQIYDRVL
                     VSIGRKPNGKNIGAENAGVAVNEWGFINVDKQQRTNVAHIFAIGDIVGQPMLAHKATH
                     EAKVAAEVIAGHKVEFVASVIPSVAYTDPEVAWVGMTETEAKAKGLEIEKASFPWAAS
                     GRALSIARTEGATKLIFDKETHRVIGAGIVGVNAGELLAEAVLAIEMGADAHDLGLTI
                     HAHPTLSETVCFAAELKEGTITDMMPPKKR"
     gene            11492..12856
                     /locus_tag="PKL42_03460"
     CDS             11492..12856
                     /locus_tag="PKL42_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNU66161.1"
                     /translation="MNSGLIVDLQQVLPSDRLFTDPVDCYAYAYDNSRNYHSPIAVVF
                     PLNIEEVASIINLCNLHKVPVVPRGRGTGTAGGSVPEVGGVVLSLERMNKIISIDPDN
                     RMAIVEPGVLNQELQDAIKGVGFFWPPDPSSAAYCSIGGNLATCAAGPHAVKYGVARD
                     HILGLKAVTGNGDIIKTGCYTSKGVVGYDLTRLLVGSEGTLAVIVEATLKLTPLPKHT
                     GGLTAEFIDTESCAKAIAAIMAQPYTPSALEFLDNGALNLIRGRHKNLLPENAKAYLM
                     IEVDGSEQEIREASAAILQACQVTGLIEAKLAHDTKALWAARKALSPLLKDIAPKKIN
                     EDIAVPVSRLPELLTGLEKLSSQYQISNVNFGHAGNGNIHVNLLVNPDNAEEMKRAYE
                     CLDEVFSLVLSLQGTLSGEHGVGMAKRPFVSREIDATTMALMKSLKLTFDPNNVLNPG
                     KLFP"
     gene            complement(13001..13675)
                     /locus_tag="PKL42_03465"
     CDS             complement(13001..13675)
                     /locus_tag="PKL42_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNU66162.1"
                     /translation="MKTTAWILLFLNIGLLVYFNLDRILPNAPTIQWAEISPEKISLL
                     TEAQISKLPKIIKPAPQAVAAPIVTPPVSACFEWGTFSLASVSKVKLALEKLSIEPII
                     NEHNAQTKKRFWVYVPPAKSAEAAQKLVGEYSTLGIDDMFIVQTPKWKNAISFGLFED
                     ETLAQSLLSTLKAKGIKNAQKSLWKQEKGDATLLLNQLSSLQVSSLKNLEVDFPEANL
                     KKVACK"
     gene            complement(13672..14502)
                     /locus_tag="PKL42_03470"
     CDS             complement(13672..14502)
                     /locus_tag="PKL42_03470"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNU66163.1"
                     /translation="MILTIDAGNSRTKWALFDDAGEVVWQGVCLNADIAAAAFLPDNQ
                     AANSTIIANVAGKAHAEKISARLQQFHINQPHWAKASAEACGLKNSYAQPETLGIDRW
                     AAMIAAWQQIQAPCVVVNAGTAVTIDGIYPISAQNPSIGEFKGGLILPGLRLMQQSLG
                     QATAQLPMPNETQTTLSNPTRHNIFAQNTADAILFGALHAIAGAITLMSHTLNAMFRT
                     WPNVVMSGGDARPIYKNMTADVTRQTIIVDNLVLQGLYLLEKEAQQHQTPRENQGKTQ
                     "
     gene            14705..>15053
                     /locus_tag="PKL42_03475"
     CDS             14705..>15053
                     /locus_tag="PKL42_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNU66164.1"
                     /translation="MPSVEVRAEKLQESASSTVNSSDTASLLENEPGVSLYRAGGVSS
                     LPAIHGLADDRIRIKVDGMDLIASCPNHMNPPLSYIDPTNIDNVKVYAGITPVSVGGD
                     SIGGTIIADTKTPL"
BASE COUNT         4016 a         3257 c         3647 g         4133 t
ORIGIN      
        1 gaacaaaaag cccaacagca atgttgggct ttttgctttt ttagtgataa gagttattgg
       61 gttcagatgt attaaaaaaa cacgattaat gattaaacgt ttgccatttt ttctaacgtt
      121 tttctgtccg taatttctac gtgcccacgc cctaaagata ctgcaccttt ttgctctagc
      181 cgtttgagtt ggcggctgac cacttctcgt gcagtcccaa gttcatcggc aattttttgg
      241 tgtgtgcggg taattgttgt ggtatttgca tcaagcagca cttttgccaa gcgtgcattt
      301 aagctgtgaa aagcgacttc atctaacagt acgattaaat cgctaatgag tgcgccgtag
      361 ttggtcatca caaacttgcg aaatactgcg gagtcgatca tgagttgatg aaaggcgttt
      421 gctgggataa gcacgtcgcg tatgggttct tcaacaattg tggaagccgg ataatcacta
      481 tcaccaagta agcacgtggt ggtgataaca caggtttcac cggcacctac gcgataaagt
      541 aaaatttctc gcccactggt cgacattttg taaacgcgag attttccagc taagcgcatc
      601 acgtaagccc cgcatggcat gccttcacga taacctatgg tgcctattgg cgcttcaaca
      661 atacgggcat atttcgctaa aatctctttt gcgtgctgat ctagttgcgc gagttctgga
      721 aatagtgaca accactcaag actactttgt atcatatgta ttaccttata ataccctatt
      781 aaaatacttg gttaatgtat tgtagcgtga atatgatgcg tttggtgagt tttcatctct
      841 taattttaat tacgttttta tttaaactta cccttgcatc attgtatttt cagggacacc
      901 gcacatgcga tttgccggta cggcaatatc gatgagcctt ggtttaggca agttaagatt
      961 attcatgatt tttacaaatt catctaatga tttgccggct aagcgcggat tgattacttt
     1021 ttcttggcca atgcaactga cgcggcggcc ttgataatca tggccggggt ataccagcgt
     1081 ttcatcaggc aggctaaaga gtttattgac gatgctattg tattgcgact cagcacttcc
     1141 gccttgaaaa tctgttctac cacaaccgtt aataagcaag gcatcgccag taaataatct
     1201 gtcgcgccat aaaaatgaaa tgctgcccag tgtatggcca ggggtggcga taactgtaat
     1261 agtttcattt gcaaatgaaa tcacatcacc atcatttaat tgtaggtctg cagtttgcgc
     1321 cccgcaagca gctgaaaccg ccgtaagagc atcagttttt tgccttagtt ttccgccccc
     1381 cgtgatgtgg tctgcatgca cgtgtgtttc aagtgagtac ttaagtgaac atttgtgagt
     1441 gttgagtaag tgtaaatatt catctatatg cgtgtttaca ggatcaatca acacagcctc
     1501 gccagcatca atatctgcga tgaaataagt ataagttgaa gagtcggtgt cgaaaagctg
     1561 tttgaaaagc atggtgtggt cactttctgg ccatatattg ccaataatat atatactcac
     1621 ataatatatt aatataatag atagtttaat atataattac ctaaacgaat aaattcaatt
     1681 tttaagtagg tatgatagac ataaaagata taaattttga tgagttacgt gaatcagccg
     1741 ataaagccag tcatttgatg cgcgtgatgg caaatgctga ccgactcatg ctactttgtc
     1801 agttgtcgca aggcgaaaag tctgttggcg aattgcagga tttacttaaa atttatcagc
     1861 ctacactttc acagcagctc acggtattac gagaggccga attggtggca acccggcgtg
     1921 agggtaaaag tatcatatac cgaatttcga gccagccagc attagcggtg atgcaggtgc
     1981 tgagtcaaca aatttgccat atttaacttt tggcattttt aagcatttat caaatttaaa
     2041 ctagaggatg catcatgacg attgaaatca acgaaattaa tcaacaattt agcacagccc
     2101 cacaactcag caccgaagac tttgcagaaa ttgcgaaact tggctataaa acgattatca
     2161 ataaccgacc tgattatgaa gggggcgacg cgcaacctaa tagtgcttcg ttaaaagcag
     2221 cggctgaagc ggctggattg gcctattttt atattcctgt gattccaaat aatattcagg
     2281 cggacgaagt agccgcattt gcccaaacgt tttcagtggc aaccaaaccc gttttaggtt
     2341 tttgtcgaac gggtaatcgt gctggtaaac tttttcaatt agcccaagta agcgcctagt
     2401 ttacctgtgt gcaaaaggcg ggtaacgggt taggcaaatg gttgacccgc aaatcattag
     2461 cattctattg gggctttttg ttggcttggt attagcgctc acaggtgctg gtggctctgt
     2521 tattgccgtg ccgttgctag cttttttctt aaacttaagc attctgcaag cggcacccat
     2581 cggcttgata gcggtcacct ttgcagcaac cataggtgct ttacatggct tacaagctgg
     2641 aatagtgcgc tataaagctg cagcactgat ggcggcattt ggtgtgctgt tagcgccagc
     2701 aggtgtatgg tttgcgcatc gcatccctaa tcaatggctc cacttggttt tttcagtcgt
     2761 acttattgct gtggcatggc gtatttggaa gcaagaccta actacaccga caatcgctga
     2821 agaaaaccct gcatttgcat gtgccgtaaa tccggcgacc tccaaattat tttggacggc
     2881 atcatgcacc aaaaggctga ttacagttgg aagcctttcc ggatttctat caggtctact
     2941 cggtgttggc ggtgggtttg ttattgtgcc aacgctgcgc aagttcagca atttagcgtt
     3001 aaacacaatt gtggcgactt cacttgctat catcgcactg gtgtcggcgg tgagcgttgc
     3061 cagttatatg ttgcacggcc aaattaactg gcaaattgca atgcctttta tgttggcgac
     3121 ggcaaccggc atgcttgctg gccgtacgtt agctggcaaa ttatcaagtc aattcacgca
     3181 acgcagcttt gcaatattgg cttggttgat tgctgcaatg ctaatttatc agtgggtaaa
     3241 catcacgttg tttggcagct aaatgttgcg caatatatca ggctaaaaag ctaaaaaacg
     3301 cgttaaattt aacacaacct ttttcgatgc tctgagagtc ttataaataa aggcttgcag
     3361 aggagaaaat ttttctcgat gtatttttaa tgtaattttg ctattttaat atgtgtaata
     3421 tagggcgtaa aaaagtcgct aaaatcagcc tcgttatcaa aatagacacc agtaaaaatg
     3481 actgaagcac ttttgttgcc atgcgcaaaa gtaaacaaat taccggctgg tttaccgtgc
     3541 attaacaaaa gcgaaagctt agcattgccg ccaaattgat tttttaaaac aatctcctga
     3601 tttttcattt caccaaccgt taaaccaatg agcgcgcctg tgcgcatcgc agtagccgta
     3661 atttttgcat cgatggcact ttgctcatcg ctaaaagtgc tatgcaagta aaagctatct
     3721 tttgtggtga cttcataggt gtagtcagtg gcatgagcca agtaattgac ttctttcaaa
     3781 aaaacgcctg ctggcgcttt ttctattgct aattcaaaag ctgcaaaatc aagctcagtc
     3841 agcagtaaag actgctcctg cgcgctcaat tgttcggttt ttgaacaacc cagcaggcaa
     3901 agcgcaaggc taaaaacaag gcatattcga agccattttt tctgcatcag atttattcca
     3961 tgaggcgttt aagcatttcc aacccaaagc caacgccaaa accgttgatg tcgctttgta
     4021 ccgcacccag cccatcttta cgattgtagg aggacaaatc catatgcagc caggcaatgt
     4081 cattttcaat aaatttgcct aaaaagcgcg cggcgtgaat atggtcaccg ccaccttcaa
     4141 gtgtgcactg tttgatgtcg gcaatgtcgc tgtctaaatc gctgtcgtag tcgtcatcat
     4201 atggaaaagc cacaatgcgc tcgcctgccg cagcgcctgc gccaaccgcc atgcaggcaa
     4261 gttcagggcg attggcaata atgccgctca tgcggtcagc caatgctgtt tgcatgctgc
     4321 ctgtcagtgt tgcaaaatcg agcatcgcat ctggcttttt acgtgaggcg agtgtgagcg
     4381 tatcggccag caccatgcgg ccttcagcat cggtatgaat aatttcaatc gtcatgccgt
     4441 tgagtgcttt gactacgtca ttctgtttgt aggcggccgg gctaatgtgg ttttgtgcaa
     4501 tggctaacca gcaatcaagt tttacgggca gtttctgcaa agtggcagcc gccaaaattc
     4561 caagagccac ggcactgccg ttcatgtctt cgtgcatgcc gtgcatgtat ttagcgggtt
     4621 ttaaattatg cccgcctgta tcaaagcaaa tccctttgcc aaccagcgca atatgttttg
     4681 ttgctttttc aggctgatac gttaaatgca caatcgcagc gtcttgcggc tcgctgcctt
     4741 ggccaacagc ataaaaagca cctgcgccca tggttttaag ggttggcata tcaaacgctt
     4801 cccaatccca gccatattcg ctggcgagcg ttttgatttt ttcacggtaa agtgttggtg
     4861 tcagttcgtt gggaggcaac ctagtgagtt gtcgacaaag cgtattgccc gccacacgcg
     4921 cattggtgaa cgcgtagttg tgatcttctt gccagccgta aatatggatg gctttgagcg
     4981 gttcatgttg atgctctttt ttgcggcttg gcagttcagc agcattcaca gcggccacgt
     5041 aataagccgc gcaggcattg gccttgcgtg ccgcctctga gccaaatacg catatcgcta
     5101 gctgctcggg ctgctcggca attaacggct taatcgcctt acgtagcaag gtgtgttttt
     5161 gaaagacatt gagcgagtta tctaaaacaa caacactggc gataccgcca tgcggtaact
     5221 caatggtgac tggcgtttta ttaaggtctt ttagcgcaga tttactgcgt tttaacttag
     5281 tctctaataa ttttgcgtaa ggaaatgcgt ctttagcatc accatccatg actaaaagta
     5341 tgtggttctc tgaagatagt gaattttcgc tacctaacgc agcattttgt actaatatta
     5401 acgacatttt agcatcctca actcttatat attatgtatg ataaacgtag caaatcttgg
     5461 gtaaataacc gcataacttc atgtttaact tgaccaaaat gcttaaaaaa gccacactta
     5521 cggctttatt aactgtaatt aacgacgcct taattcacgt ttagttgcta tagttttgtt
     5581 aattatacgc attgcgacta caacacggag aaatctcacg catgacattc aatcagcaaa
     5641 caggattgca agaaacagac gcgcaggaaa cacaagaatg gttagatgcg cttgcctcag
     5701 tgattgaaac tgaaggtact gaacgcgcac actttttact tgaggcaatg attgaccaag
     5761 cgcgccgctc tggcagcaat ttgccctata acgccaccac cgcttatgtg aatacgattc
     5821 ctacacattt gcaggcaaaa ttaccgggcg accctgaaat ggagcgccgt gtgcgcgcac
     5881 tagtgcgctg gaatgcgatt atgaccgtcc tacgcgccaa tgaaaaatcg cctggcgtcg
     5941 gtggccacat tgccagcttc caatctgctg ccactttgta tgacactggg ttcaattatt
     6001 tcttccgcgc gccaaatgag aattttggtg gtgactgtgt gtactttcaa ggtcactcat
     6061 ctccaggtgt atacgcacgt gctttcctag aaggccgctt tactgaagag caaatggata
     6121 attttcgcca agaaacaggc ggcaatggct tatctagtta cccgcatcct tggttaatgc
     6181 cggatttttg gcagttccca acagtgtcta tgggtttagg gcctttggca ggtatttatc
     6241 aagcgcgctt tttaaaatat ttacatgacc gtggcattgc cgacacctct gaccgcaaag
     6301 tgtgggtgtt ctgtggtgac ggcgagatgg acgagcctga atcgcaaggt gcgatttcat
     6361 tagcttcacg tgagaaatta gataatttaa tctttgtgat taactgtaac ttgcaacgtt
     6421 tagacggccc agtgcgtggc aacggcaaaa ttattcaaga gttggaatca aatttccgcg
     6481 gttcaggctg gaatgtgctg aaagtgattt ggggttctta ttgggatccg ctgctggcca
     6541 tggataaaga tgggttgctc aaaaagcgca tggaagaatg tgtggatggc gaataccaaa
     6601 actttaaagc taaaggtggc gcctatacgc gtgaacattt cttcggcaag tatccgcaat
     6661 tgaaagaaat ggttgctgcg atgtccgata gcgatatttg gcgtttgaac cgcggcggcc
     6721 atgacccgca caaagtgtat gcagcttata acgcagcggt aaaccacaaa ggtcaaccaa
     6781 ccgtgatttt agccaaaact gttaaaggtt atggtatggg gaatgcgggc gaggcgcaaa
     6841 atacgacgca tcagcaaaaa agcatggata ttgaatcgct aaaaaaattc cgtgaccgct
     6901 ttgatttgcc tatcaccgat gagcaagttg aaaatctatc gttttacaaa ccggcagaag
     6961 atagcccgga aatgcaatac atggcagcac gccgtgccgc gatggggggc tttgtgccac
     7021 aacgccgtcg caaaggtaac gcgttaaccg tgcctgaatt gtcagcattt gaaaatatgc
     7081 tggcagccac aggtgaacgt gaaatttcaa ccaccatggc gtttgtgcgc attctttcaa
     7141 cattggtacg cgataaacaa attggtaaat atgttgtgcc gattgtgcct gacgaagccc
     7201 gcacatttgg tatggagggc ttattccgtc agttagggat ttattcatcc gctgggcaac
     7261 tctacgaacc aatggattct gatcaagtga tgttctacaa agaatcgaaa gatggtcaga
     7321 ttcttgaaga gggtattaac gaagcgggtt catttgccag ctggttggct gccgccacgt
     7381 catactcagt gacgggcaca cagatgattc cattctatat ttattattca atgttcggct
     7441 ttcagcggat tggcgattta gcatggatgg cgggcgatag ccgtgcgcga ggcttcttgc
     7501 tgggagctac cgctggtcgt actacattaa atggcgaagg cttgcagcat gaagatggtc
     7561 atagccattt aatgtcagcc atgattccaa actgtgtttc atacgatccg acttttgcgt
     7621 atgaattagc cgtgattatt caagaaggct tgcgccgtat ggtgcaaaac caggaggatg
     7681 tgtattacta catcactttg atgaatgaaa actatagcca cccagcgttg ccaaaagggg
     7741 ctgaagaagg catcttgaaa ggcttataca gtttcagtaa atcaaaagcc aaaggcgaaa
     7801 aagtacaact catgggttcg ggcgtaattt tacgtgaagt gattgccgca gctgaattgc
     7861 tggaaagcga ttggggtgtg tctgcagatg tgtggagcgc caccagcttt accgaattac
     7921 ggcgtgaggg cttggattgt gagcgctgga acttgctcaa tccagaaaaa ccacaaaagg
     7981 ttaattttgt ggcgcaaagc ttaaaagacg ccaaaggccc ggtgattgcg tcaacagatt
     8041 acatgaagag ttttgctgaa cagattcaac gtttcgtacc aaataaattt gtggctttag
     8101 gcaccgatgg ttatggccgt tctgacagca gagaagcctt gcgtgatttc tttgaagtaa
     8161 accgatacta cgttaccgtg gctgcgttaa aagcactgag tgatgaaggc aaattgcctg
     8221 tttcaaaagt ggcagaggcg gttaaaaagt acaagttgaa cgcaaaaaaa ccaagcccaa
     8281 cgagtattta aaaaagcatt gaaggactaa aaaaatggca ttaaaagacg tactcgttcc
     8341 tgatattggt aactttgatt cagtggatgt aattgaagtt ctaattaaaa ctggtgacac
     8401 catcgccaaa gatgattcgc tcattaccgt ggaatctgac aaagcgtcga tggatattcc
     8461 tgcgccgttt gccggcgttg tgaaagaagt caaagtaaaa attggcgaca aagtggctga
     8521 aggcacttta attttaacgc ttgaggcaag tgatgttgct actgccacag ttgaggttaa
     8581 accagcatca gtgcctgaag tgcctgaagt gcctaaagcc gcagcgccag ttgcggcgat
     8641 tccagagcct agtcgccctg cgccagagcc gccaaagcaa gtgcagccag cgcaacagcc
     8701 agcaccagtg ggtgagcatg tggtggtggc tgctggcaag ttgtcccacg ccagcccatc
     8761 cattcgtaag tttgcccgcg agataggtgt taatctcgcg ttggtgaagg gttctggccc
     8821 aaaaaaccgt attttgcaat cagatgtaca gagttttgtt aagggtgaac tggctaaacc
     8881 gcgtaccgag aatatgggag ctggcttggg cgcgctcgca accttaccga tgccagtgat
     8941 tgatttcagt cagtttggcg cgattgaaac taaaccgctt tcacgcatta aaaaattgtc
     9001 gggtgcaaac ttgcaccgca attgggtaac cgcgccacac gtaacgcaat ttgatgaagc
     9061 ggatattact gacttggaag attttagaaa atccatgcag gccgacgcag aaaaacgtgg
     9121 cgttaaactt accatgctag cctttttgat taaggcttca gtcaatgcgc taaaagccta
     9181 ccctaatttc aacgcttcac tttcacccga tggcgatagt ttgatattaa aaaactatta
     9241 caacattggc tttgcctgcg atacgcctga cggcctagtg gtgccagtgg tgaaagatgt
     9301 gcaaagtaaa gatgtgcttg atattgctcg cgacttaatg gagctctcca ccaaagcgcg
     9361 cgaacgcaaa ttaaaagtgg aagaaatgca gggcggaacc ttcaccattt caagtttggg
     9421 cggcattggt ggcaccatgt ttacgccaat tattaattgc cctgaagtgg cgattttagg
     9481 tgtttcacgt tcaagtatgc agcccgtgta cgacaaaaca accaacggtt ttgagccacg
     9541 cttgatttta ccgttatcgc tttcttatga tcaccgcgtg gtcgatggtg cggacggtgc
     9601 gcgctttacc agccatatgc gcatgatgct cagtgatgta cgtcgcctgt tgctctagtt
     9661 gcaaattatt caaaaaattt agaaatgcaa aatcatgatt gaaattaaag tccccgatat
     9721 tggcaatttt gacagcgttg atgtgataga cgtgttagtt aaagtgggcg ataccattgc
     9781 aaaagaagac tcgctcatta ccgttgagtc tgacaaagcg tcgatggata ttcctgcgcc
     9841 tcaagccggt gttgtaaaag aattgaaaat caaagtgggt gataaagttg caaaagactc
     9901 actaatttta ttgcttgaaa ctgacggagt aaaaaatgat gccccagaag ccttgcagaa
     9961 taaggcttcc agcccggtag cagagaaggt agctgaaaaa gcagttgaaa acccggtgac
    10021 agcccctatt ccagcagtcg ccacaagtgg caatgacatc agcactgagg ttgtggtgct
    10081 gggttcaggg ccaggtggtt acacggcggc gttccgcgcg gcagacttgg gcaaaaaagt
    10141 cgtcttgatt gaacgctatg caacgctggg cggcgtgtgc ttaaacgtcg gttgtattcc
    10201 ttccaaagcg ttattgcaca ctgcaaaagt gattaccgaa gcccaagaaa ctgcgcacca
    10261 cggcgtgtct tttggtgcgc caaaagtgga tttagatacc ttgcgcaatt ggaaagccaa
    10321 cgatgtagtg ggcaaactca ctggcggctt ggcggcgatg gctaaggggc gcggtgttac
    10381 tgtggtgcaa ggcgttggca aatttactag tccaaatcaa attgccgtga ctttaaatga
    10441 tggttcagca gcggatggca aagtgaccac tatcggtttt gaacatgcca ttattgccgc
    10501 aggctctcaa gccactaaat ttcccggtgc gccagatgat gagcgcatta tggattccac
    10561 cggcgcgttg gcgttagccg acattccaaa acgaatgttg gtgattggtg gcggtattat
    10621 cggcctagaa atgggcaccg tgtacgacgc gctcggcacc aaagtgagcg tggtggaatt
    10681 tatggatggc ctgattacag gttgcgaccg cgacttggtg cgtcctttgc aaaagcgcat
    10741 ggaaaaacgc tttgaaagta ttatgctctc caccaaagtc tcaaaaattg aagcaaaaaa
    10801 agatggcatt catgtgagct ttgaaggtga gaatgcgcct gatggcgtgc aaatttatga
    10861 ccgtgtattg gtttctattg gtcgtaaacc taacggcaaa aacataggcg cagaaaatgc
    10921 tggtgtggct gtgaatgagt ggggctttat caatgtggat aaacagcaac gcactaatgt
    10981 agcgcatatt tttgccattg gtgacattgt tggccagcca atgctcgcgc ataaagccac
    11041 gcacgaagcc aaagtggcgg cagaagtgat tgcgggccat aaagtagaat ttgttgcgag
    11101 cgtcattcca tctgttgcct atacggatcc tgaagtggct tgggtaggca tgaccgaaac
    11161 tgaagccaag gccaaaggtc ttgaaattga aaaagcatca ttcccttggg cagctagtgg
    11221 acgcgcactt tcaattgcac gcactgaagg cgctactaaa ctgatatttg ataaggaaac
    11281 gcaccgtgtc attggcgcgg ggattgtggg cgtaaacgca ggtgaattgc tggctgaagc
    11341 ggtgctggcc attgaaatgg gtgcggatgc gcatgactta ggtttaacca tccacgcgca
    11401 cccaacgcta tctgaaaccg tgtgttttgc ggcggagtta aaagaaggca ccatcactga
    11461 catgatgccg ccgaaaaaac gataaattca gatgaattca ggcttaatcg tcgatttaca
    11521 acaggtgctg ccatcagata ggctgttcac tgaccctgtc gattgttacg cttatgcata
    11581 cgataattcg cgtaattatc attcgcccat cgccgttgtt tttccgctga atattgaaga
    11641 agttgccagc attatcaatc tgtgcaattt gcataaagtg cctgttgtgc cacgtgggcg
    11701 cggcactggc acggcgggcg gcagtgtgcc tgaagtcggc ggcgtggtac tgtcgcttga
    11761 gcgcatgaac aaaattataa gcattgaccc agataatcgt atggcgattg ttgagccggg
    11821 tgtgttgaat caagagctgc aagatgccat taaaggcgtt ggcttttttt ggccgcctga
    11881 cccgtcaagt gcggcctatt gtagcattgg cggcaatctt gccacttgcg ctgcaggccc
    11941 acacgcggtg aaatacggcg tggcgcgcga ccatattctt ggcctaaaag ccgttaccgg
    12001 caatggcgac attatcaaaa cgggctgcta caccagcaaa ggcgtggtgg gttacgattt
    12061 aacacgattg ctcgttggca gtgaaggcac attagcggta attgttgagg ccacgctcaa
    12121 gcttacgccc ttacctaagc atacaggcgg gttaacggca gaatttattg acaccgaaag
    12181 ttgcgctaaa gccatcgccg ccattatggc gcagccttac acacctagtg cgctagaatt
    12241 tttagataac ggcgcgctca atttaatacg tgggcgacac aaaaatctgc tgccagaaaa
    12301 cgcaaaagcc tatttgatga tagaagtgga cggcagtgag caggagattc gtgaggccag
    12361 cgcggctatt ttgcaggctt gccaagtcac tggcttgatt gaagccaaac tcgcgcacga
    12421 caccaaagca ctttgggcag cgcgtaaagc cttgtcgcct ttgcttaaag acattgcgcc
    12481 gaaaaaaatc aatgaagaca tcgcggtacc ggtgtcgcgc ctgcctgaac tgctcacggg
    12541 tttagagaaa ttgagcagcc aatatcaaat cagcaatgtg aattttggcc atgccggtaa
    12601 tggcaatatt catgtgaatt tgctggtgaa ccctgataac gctgaagaaa tgaagcgtgc
    12661 gtatgaatgt ttggatgaag tgtttagttt agtgttatcc ctgcaaggca cgctctcggg
    12721 cgagcatggc gtgggcatgg ccaagcgtcc ttttgtatcg cgcgagatag atgccaccac
    12781 catggcgcta atgaaatcgc taaagctcac ttttgacccg aataatgtgt taaatccggg
    12841 caaacttttt ccttaaaacg ccgtttttta aagacgtttt tgtgaccata aatacgccga
    12901 ggaaaaaata atgctctgcg agcctttatt tataaggctt gcagagcatc gaaaaaggtt
    12961 ggtgcttaaa atggcattaa aaagccattt ttcacaggct ttatttgcag gccacttttt
    13021 taagatttgc ctcaggaaaa tcaacttcca agttttttag tgaactcact tgcaacgatg
    13081 aaagttgatt caacaatagc gttgcatcgc ctttttcttg cttccaaagt gatttttgcg
    13141 catttttaat gcctttggct tttaaggtgc ttaataacga ttgcgccagt gtttcatctt
    13201 caaacaggcc aaaagaaatc gcatttttcc attttggtgt ttgtacaata aacatatcat
    13261 caatacctag cgtgctatat tcgccaacca acttttgtgc cgcctcagca gatttggctg
    13321 gcggcacata aacccagaaa cgcttcttgg tctgggcatt atgctcgtta atgatgggct
    13381 cgatagaaag tttttctaag gctaatttca ctttacttac actcgcaagt gagaaagtac
    13441 cccattcaaa acaagcgctt acaggcggcg taacaatagg cgctgcaacg gcttgcgggg
    13501 ctggcttaat gatttttggc agtttgctaa tttgcgcttc agtgagcaag ctgatttttt
    13561 ctggtgaaat ttcagcccat tgtatcgtgg gtgcattggg taagatgcgg tctaaattaa
    13621 aatagaccag taggccaata tttaaaaata gtaatatcca agctgttgtt ttcattgcgt
    13681 tttaccttga ttttcgcgcg gtgtttggtg ctgctgcgct tctttttcaa gtaaatataa
    13741 cccttgtagc actaaattat caacgatgat ggtttgtctt gtcacgtccg cagtcatatt
    13801 tttataaatg ggtcgcgcat cgccgccgct cattaccaca ttaggccaag tgcgaaacat
    13861 cgcgttgagc gtgtgagaca ttaaagtgat tgcacccgca atggcgtgta gcgcgccaaa
    13921 aagaatggca tcggcggtat tttgcgcaaa aatattgtgc cgcgtaggat tgctaagggt
    13981 cgtttgtgtc tcatttggca taggcagttg tgctgtggct tggcctaaac tttgctgcat
    14041 taagcgcaac cccggcaaaa taaggccgcc tttaaattcg ccaatgctgg gattttgtgc
    14101 agatatgggg taaatgccat caatggtcac cgctgtacct gcattcacta ctacgcacgg
    14161 cgcctgtatt tgttgccaag ctgcaatcat ggccgcccag cgatcaatgc ctaatgtttc
    14221 tggctgcgcg taactatttt taagcccaca ggcctctgca ctggcttttg cccaatgtgg
    14281 ttgattgatg tgaaactgtt gtagccgtgc tgagattttt tctgcgtggg cttttcccgc
    14341 aacattggca ataatcgtgc tgttggcggc ttggttatca ggtaaaaatg cagcagcagc
    14401 aatgtctgca ttcaggcaga cgccctgcca aaccacttct cctgcatcat caaagagcgc
    14461 ccatttagtg cgggagttgc ccgcatcaat cgttaatatc ataagtggtt aacctgactt
    14521 ttggcttaag tgcgacaatt tgccacattc tcagcgttac cctaaaaagg taaaatatcc
    14581 ttttttctaa tagggtattc gtatcatgca acattttagg cttaagcgaa atattttatc
    14641 gagcatttta gcaccttgtg tgctgagtgt cgtttcaagc gttgcatggg cggatgattt
    14701 aacaatgcct agcgtagaag tacgtgctga aaagctgcaa gaaagtgcat catcgactgt
    14761 taatagtagt gatactgctt cgttacttga gaacgaaccg ggtgtaagct tgtatcgcgc
    14821 gggtggtgtg tcgagcttgc cggcgataca tgggttagcc gatgaccgca tacgcatcaa
    14881 agttgatggt atggatttga ttgcttcctg tcctaaccac atgaacccac cgctttcata
    14941 tatcgaccca accaatattg ataatgttaa agtttatgct ggtatcactc ctgtgagtgt
    15001 gggtggcgac agcattggcg gaacaattat cgcagataca aaaaccccac tat
//
LOCUS       DAOQSI010000031        14918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_3074_length_14918_cov_5.719639, whole genome shotgun sequence.
ACCESSION   DAOQSI010000031 DAOQSI010000000
VERSION     DAOQSI010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 14918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14918
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3074_length_14918_cov_5.719639"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3335
                     /locus_tag="PKL42_03480"
     CDS             <1..3335
                     /locus_tag="PKL42_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HNU66165.1"
                     /translation="VKNALASAKPYQQWLKKSRYFLCDMPKIESDFKLTASLLDIQQA
                     FGYTQEDIKFVLQPMVQTGEEAYGSMGNDAALPVLSAKPKLLYNYFKQLFAQVTNPPV
                     DPIREELVMSLVTFIGPKPNLLAVDETAPSIRLEASQPVLTLNELEQLKAIATLTQNQ
                     HKSLLLDITYPAAQGKAGMAAAVASVTRAAEQAVKDGFNILILSDRTVSEARLAIPAL
                     LACSATHKHLVQAGLRTSTGLVVDTGSAREVHHFALLGGYGAEAVCPWLAFETIKHLE
                     RKNTDEAAKKFVKATGKGLYKVMSKMGISTYQSYCGAQIFEAIGLNSAFVEQYFTGTA
                     THIEGIGLDQVAEETLRLHSAAFGADPLLANNLDAGGEYAFRVRGEEHTWTPDSIAKL
                     QHATRTGQYETYKEYAKLINDQSRRHMTLRGLFEIKPANLPIPLYRVESAKEIVKRFA
                     TGAMSLGSISTEAHATLAIAMNRIGGKSNTGEGGEDPKRFIPVTSGTSVAAVIGRHLI
                     ETDIPLNVGDSMRSAIKQVASGRFGVTAEYLSNADQIQIKMAQGAKPGEGGQLPGHKV
                     SPYIAQLRFSVPGVGLISPPPHHDIYSIEDLAQLIHDLKNANPSATISVKLVSETGIG
                     TVAAGVAKAKADHIVVAGHDGGTGASPISSVKHTGTPWELGLSETQQTLVLNQLRGRV
                     VLQVDGQIKTGRDVLIGALLGADEFGFATAPLVVEGCIMMRKCHLNTCPVGVATQDPE
                     LRKRFTGQPEHVVNYFFFVAEEVRELMASMGIEKFDDLIGRADLLDMQAGIDHWKLQG
                     LNFSKVFHQPEMPASVSRKHNDVQDHGLANALDNKLVKLAKPALERREQVVLDLPISN
                     TNRTVGTMLSHQIAKRYGQAGLPDDTIQVKFTGTSGQSFAAFLAQGISFELTGEGNDY
                     VGKGLCGGRIVIKPPAAFRGVSHENIIVGNTVMYGATTGESYFSGVAGERFCVRNSGA
                     SAVVEGVGNHGCEYMTGGTVVVLGVTGQNFAAGMSGGVAYVYDEDGQFAKRCNMGMVS
                     LEKVQHISQVSADAIHHLGTPDETILLGLLAKHIAYTNSERAKIIVQYWDEHREKFVK
                     VMPNEYKRALIELAEDKVLEAV"
     gene            3335..4762
                     /locus_tag="PKL42_03485"
     CDS             3335..4762
                     /locus_tag="PKL42_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HNU66166.1"
                     /translation="MGKVTGFIEYNRLSEANLPVPERVKNYKEFVLHLTDEQAQTQGA
                     RCMDCGIPFCTTGCPINNIIPDWNDLVYHQDWRSAIDVLHSTNNFPEFTGRICPAPCE
                     AACTLNINDDAVGIKSIEHAIIDKAWENNWVTPQINPNKTGKKVAVVGSGPAGLAAAQ
                     QLARAGHAVVVLEKNSRIGGLLRYGIPDFKMEKSHIDRRMAQMTAEGVEFRVNQHVGD
                     NVSADDLVAEFDAVLLAAGAEQPRDLPVKGRELDGVHFAMDFLTPQNKVNAGDVIPNQ
                     IKATGKHVIVIGGGDTGSDCVGTSNRHGAASITQFELMPQPPEKENRALVWPNWPLKM
                     RTSSSHEEGANRDWSITTKELMGENGKVEALKGARVAWKDGKMTEIAGSEFTIPADLV
                     FLAMGFVSPVEKLLAVFGVEKDQRGNAKATTEGATSYQTSKAKVFAAGDVRRGQSLVV
                     WAIREGRQAAQAIDAFLMGASVLPR"
     gene            4832..5896
                     /gene="hemE"
                     /locus_tag="PKL42_03490"
     CDS             4832..5896
                     /gene="hemE"
                     /locus_tag="PKL42_03490"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HNU66167.1"
                     /translation="MTTLQNDTFLRALMRQPTDYTPVWMMRQAGRYLPEYRESRKTAG
                     SFLQLCKNAQFATDVTLQPLDRYPLLDAAILFSDILTVPDAMGLGLYFEEGEGPKFER
                     TLQLEADIQKLVVPDMAKLQYVFDAVSSIRKALNGRVPLIGFSGSPWTLATYMVEGKG
                     GTDFLTTKKMAYARPDLLHHILETTAQTVIQYLNAQIAAGAQAVMIFDSWGGALSHHA
                     YTEFSLNYMQKIVAGLTKNAEGKKVPSIVFTKGGALWLEAQADIGADALGLDWTVDIG
                     SARQRVGDKVALQGNLDPAILLSTPQAIEREVASVLASYGKGHGHVFNLGHGITQWTP
                     PENAACMLNAVRELSRQYHA"
     gene            complement(6106..6705)
                     /locus_tag="PKL42_03495"
     CDS             complement(6106..6705)
                     /locus_tag="PKL42_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HNU66168.1"
                     /translation="MNQVSTRYTKVAVILHWLIALVVFGMFALGWFMSELPKDAPKQL
                     GFDLFDLGLYTWNLAEEASPRTFYFNLHKSIGVTLLALIALRLVWRITHRPPAMLTSY
                     KAWERKLATGTHHVLYLLMFAVPLSGLIMAINSKYGLMWFGIDFIAGLDNKDIRELFK
                     EVHEIIGFVFLVVIALHIAGALKHKFIDKDGTMKRMSLK"
     gene            6812..7753
                     /locus_tag="PKL42_03500"
     CDS             6812..7753
                     /locus_tag="PKL42_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein relay system zinc metallohydrolase
                     2"
                     /protein_id="HNU66169.1"
                     /translation="MRILMIIMSMLAFVGCAHSQPNQTASVNHDDFAIENVGDGIYVH
                     HGAHLDIDTGYQGDICNISFVVGSRGVAVIDTGGSLKVGQQLREAIRKITPLPVLYVI
                     NTHVHPDHIYGNAAFLTDKPQFIGHEKLANAMATREEQYAKLNVQLLGEDAKGSELVK
                     PTMAVKTTLEIDLGDRQLRLTAHPVAHTNTDVSLVDSKTSTLFAGDLLFIDRTPVIES
                     DIEGLIAELEKFKAGQFKQVVPGHGPVAKDWLAAINNAERYLNVLLADIRASIKKGES
                     MESAMNTAAASEKEKWVLFDIANRRNVNILYPALEWE"
     gene            complement(7884..8252)
                     /locus_tag="PKL42_03505"
     CDS             complement(7884..8252)
                     /locus_tag="PKL42_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNU66170.1"
                     /translation="MQKYLGTLGRVLLAQVFLLQVIILIVGFFNTPDGYQQYQAGLGS
                     LGLPGIFAPLIILVQLVGGLALFLGFKTKFFALLMAIYAVIISYLLHLPVLQYLAIAG
                     GLITLYVNSNTAFSLDNRNK"
     gene            8328..8813
                     /locus_tag="PKL42_03510"
     CDS             8328..8813
                     /locus_tag="PKL42_03510"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNU66171.1"
                     /translation="MKNWQLITTVSLSARATEPNAASKISIFQVSWQAAQHELQAVRT
                     PVFVVEQLVSPDFEWDEIDAQAVHLLVMHHAQPIACLRIIAYQKIGRMAVLKQWRNKG
                     LGKALLEEAVRICRAQGSASVRLSAQTHAVQFYQKAGFKQTSEEYTDVHIPHVDMQLD
                     L"
     gene            8888..9439
                     /gene="fae"
                     /locus_tag="PKL42_03515"
     CDS             8888..9439
                     /gene="fae"
                     /locus_tag="PKL42_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HNU66172.1"
                     /translation="MSKIIFKAGEATVYSEGKDVTAAMPEILIGAVDGPVGQAFANLM
                     AQSKGHTAMFAVRDINQLVRPVCMTVPKVTLKGSTDVGLFGGVVQAATADAILDCVIE
                     GIIPKDQANDLCIISLVWIDPGCIPLEKEGKLDKADMYKNNYDATKLAIKRALNDEPS
                     IDELIANRKKIKHCMWEESWGEI"
     gene            9537..9767
                     /locus_tag="PKL42_03520"
     CDS             9537..9767
                     /locus_tag="PKL42_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66173.1"
                     /translation="MAEHNVCKEAFDRLCDEVNTDKKSAINPDDYWLFELGFRSAIEE
                     LLSIADAGEQARKFVSPRFQMLADKILNSRLH"
     gene            10052..10561
                     /locus_tag="PKL42_03525"
     CDS             10052..10561
                     /locus_tag="PKL42_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HNU66174.1"
                     /translation="MPSMYQHTIPPLKRALRNLAAILKKGAAYAEAKNIEPGVLLNAR
                     LFPDMYPLVRQVQIATDMSKGAAARLANVEIPKYEDNETTFDALQARIAKTIAFIDSI
                     QPAQLEGSESREVVISVRKIELKFTGLDYLLKWVNPNVYFHVTTAYNILRHNGVELGK
                     SDFLGPRKD"
     gene            complement(10571..10786)
                     /locus_tag="PKL42_03530"
     CDS             complement(10571..10786)
                     /locus_tag="PKL42_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HNU66175.1"
                     /translation="MNSDDLQRLVTLAMPYGKYKGRLIADLPGHYLNWFAREGFPKGD
                     LGRLLQLMQEIDHNDLSALLEPLRNKG"
     gene            complement(10952..11704)
                     /locus_tag="PKL42_03535"
     CDS             complement(10952..11704)
                     /locus_tag="PKL42_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66176.1"
                     /translation="MRNTALYTTCLLAFWFSASAQATPCLDANALEKMRQNELNYLLN
                     RVPPAFKHAVDDGKITLTMALAEGVACRAQATFNLPADDLAEGNKVLEADPAKRIILF
                     SQGYALPESTTVSAQFEVDSGTLAVSHQDILQTAELGKLRASIEMLYATLSQSRAVLA
                     QNQTNSLAWPKEFRDNEIAQCSARAKATNVTEACTCKIDALAKVVSARQVEYQTYLRS
                     NPYASATGAGNTFNALEQQVSQDCGLQLANAK"
     gene            complement(11715..12215)
                     /locus_tag="PKL42_03540"
     CDS             complement(11715..12215)
                     /locus_tag="PKL42_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNU66177.1"
                     /translation="MLATNAFAADCPTLLNHQFKTLQGNTINLCDYRGKPILVVNTAS
                     KCGFTPQFEKLEGLYSRYKDNGLLVIGFPSNDFKQELTSDSEIGSFCKLTYGVKFPMV
                     SKGSVKGENANPFYKQLIKASGTEPQWNFHKYVISADGKTVQSFKSGVEPDDAAILKL
                     IEPALK"
     gene            12376..13005
                     /gene="umuD"
                     /locus_tag="PKL42_03545"
     CDS             12376..13005
                     /gene="umuD"
                     /locus_tag="PKL42_03545"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HNU66178.1"
                     /translation="MSENHPIHLSKIAPRKNKPGGGRKVGTGKFGEATAVMRVPASQT
                     LIIKDFLAAYQQKMRATNLDAVREFEWPADEVKAVDLPLYSSKVSAGLPSPAEEHVEK
                     RLDPNEFLIDQKDATFFVTIQGMSMVDVGLLPGDKAVVDRSKLPSIGDIVLAVLDGEF
                     TIKTLSRSKHGNVRLLPANATGAYSPIEITENMNFEIWGVVTGSFRRFK"
     gene            complement(13080..13151)
                     /gene="pqqA"
                     /locus_tag="PKL42_03550"
     CDS             complement(13080..13151)
                     /gene="pqqA"
                     /locus_tag="PKL42_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HNU66179.1"
                     /translation="MWTTPAATEMRFGFEVTMYVMNK"
     gene            complement(13300..13731)
                     /locus_tag="PKL42_03555"
     CDS             complement(13300..13731)
                     /locus_tag="PKL42_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66180.1"
                     /translation="MNMKFVYSLAALTVLCPMLYWVANTFDWFSADQPKAEPSAMSLA
                     LNSCSGIADKSVANLTAVVEYQKLEIAGRRARVLQNCMNDQGFTENPAWVKATQPLAQ
                     AAANTQNISLDEAQENLKRHDMEAFYPSQDAPLYWLAKPAQ"
     gene            complement(13757..14791)
                     /locus_tag="PKL42_03560"
     CDS             complement(13757..14791)
                     /locus_tag="PKL42_03560"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNU66181.1"
                     /translation="MKTAKLLYLLGLVVPFASAAPPAEPTDAFIYQLKESLVKVAVNT
                     KSGGHGFGTGVAVSKDHIVTNCHVVDNANGISVSKWGVEYAPVSMQADWKHDLCILKF
                     EWADFKPVKIAESDAMQYEQPVISISMPSDSPAPYVELSAIKALYPMDDGEVIRTKAA
                     FAIGASGSPVFDYSGNLIGISTFKSPGRNAYFYNMPVKWVRALLQQPAMQFDAAHALP
                     FWDAPEDGRPFFMQIVLPFQNNRWADVQKIASNWRNAEPNNAEAWFYLGAAAQNQNDS
                     TNALKHFNKALALHPNHPASLQALALMAHQQGNAAEVNTIRAVLKTVSIDTLQSLDDA
                     LGALQQCATC"
BASE COUNT         4195 a         3271 c         3587 g         3865 t
ORIGIN      
        1 aagtcaaaaa tgcacttgcc agcgccaaac cttaccagca atggctgaaa aaatctcgct
       61 actttttgtg cgatatgcca aaaattgaaa gtgattttaa attaaccgca agcttgctgg
      121 acattcagca ggcctttggt tacacacaag aagacattaa atttgtattg cagccgatgg
      181 tgcaaacggg cgaagaagct tatggctcga tgggtaacga tgctgccctg cctgtgttat
      241 ctgcaaagcc aaagcttttg tacaattact ttaagcaatt attcgcacaa gtgaccaacc
      301 cgccagttga cccgattcgt gaagaattgg taatgtcgct ggtgactttt attggcccca
      361 aacctaactt gttagccgtg gatgaaaccg caccgtcgat tcgattagag gccagccagc
      421 cagtactcac gttaaatgag ctggaacagt taaaggcgat tgctaccctc acgcaaaatc
      481 aacacaaatc tttgctgctg gacattactt accctgccgc gcaaggcaag gcgggcatgg
      541 cagctgccgt tgccagcgtc acccgcgcag cagagcaagc agttaaagac ggtttcaata
      601 ttcttatact ttcagacaga actgtgagtg aagcgcgctt ggcgattcct gccttgctgg
      661 cttgttcagc aacgcataaa catttggtac aagcgggctt aagaaccagc actggtttgg
      721 tggttgacac aggctcggca cgcgaggtgc atcattttgc gctgttgggc ggctatggtg
      781 cagaagccgt ttgcccgtgg ctggcgtttg aaaccatcaa acacctagag cgcaaaaaca
      841 cagatgaagc cgccaagaaa tttgtaaaag cgactggcaa aggcttgtat aaagtcatgt
      901 ctaaaatggg catttcgact tatcaatcgt actgtggtgc gcaaattttt gaagcaattg
      961 gcttaaacag tgcattcgta gagcaatact ttacaggcac agccacgcat attgaaggca
     1021 ttggcttaga ccaagtagcc gaagaaacgt tacgcttaca tagcgcagcc tttggtgcag
     1081 acccattgct ggcaaataat ttggacgcag gcggtgaata cgcgttccgc gtgcgcggtg
     1141 aagaacatac ctggacgcca gattccatcg ccaagctgca acacgcaacg cgcaccggcc
     1201 aatatgaaac gtataaagaa tatgccaagt tgattaacga ccaatcacgc cgccatatga
     1261 ccttgcgtgg cttgtttgaa attaaacccg ccaatttgcc gattccgctt tatcgcgttg
     1321 aatctgccaa agaaattgtt aaacgttttg ccactggcgc gatgtcgctg ggctcaattt
     1381 caaccgaagc ccatgcaacg ctggcgattg ccatgaaccg cattggcggc aaatcgaaca
     1441 cgggtgaagg cggtgaagac ccgaaacgct ttattcctgt aaccagtggc accagcgtgg
     1501 ccgccgtgat tgggcggcac ttaattgaaa ccgacatccc gctcaatgtg ggcgattcca
     1561 tgcgctctgc gattaaacaa gtggcttctg ggcgctttgg ggttacggca gagtatttat
     1621 ccaatgccga ccaaatccaa atcaaaatgg cgcaaggtgc aaaaccgggt gaaggcgggc
     1681 aactacctgg ccataaggtc agcccgtata ttgcacaact acgtttttct gtgccaggtg
     1741 tgggcttgat ttcgccgccg ccgcaccatg atatttattc gattgaagat ttagcacagc
     1801 tgattcatga tttaaaaaat gccaacccaa gtgccacgat ttcagtgaag ctggtgtcag
     1861 aaacaggcat cggcaccgta gcggcaggtg tagctaaagc aaaagccgac cacattgtgg
     1921 ttgcggggca tgatggcggt acaggcgcat cgccaatttc atcggtaaaa cataccggta
     1981 cgccatggga gttggggctt tctgaaaccc aacagacgct ggtcttgaat caattgcgtg
     2041 gccgtgttgt gctgcaagtg gacggccaaa ttaaaacggg ccgcgatgtg ctgattggcg
     2101 cattgctggg tgccgatgag tttggttttg ccaccgcgcc cttagttgtg gaaggctgca
     2161 tcatgatgcg taaatgtcat ctcaacacgt gcccggtagg cgttgccacg caagaccctg
     2221 aactgcgtaa acgctttaca ggccagcctg agcatgtggt gaattatttc ttttttgtgg
     2281 ctgaagaagt gcgcgagctg atggccagta tgggcattga aaagtttgat gatttaattg
     2341 gccgtgccga tttgctggat atgcaagcgg gcatagatca ttggaagctt cagggcttaa
     2401 atttcagcaa agtatttcac cagccagaaa tgcctgccag cgtgtcacgc aagcacaatg
     2461 atgtgcaaga ccatggtttg gctaacgcgc tggataacaa attggtcaaa ctcgcaaaac
     2521 cagcgttaga aagacgcgag caggtggtgt tagatttacc tatttccaat acaaatcgta
     2581 ctgtgggcac gatgttgtcg caccaaattg ctaaacgtta cggtcaggca ggcttgccag
     2641 atgacaccat acaagtgaaa tttactggta cttcaggcca aagttttgcg gcatttttag
     2701 cgcaaggcat tagctttgaa ctcacgggcg aaggcaacga ttacgtaggt aaaggcctgt
     2761 gcggtggcag aattgtgatt aaaccgcccg ctgcattccg cggtgtttcg catgaaaaca
     2821 tcattgttgg caacactgtg atgtacgggg caaccacagg cgaaagctat ttcagtggtg
     2881 tggcgggcga gcgtttttgt gtgcgtaatt ctggcgcatc agcggtggtc gaaggcgtgg
     2941 gtaaccacgg ctgcgaatac atgacaggcg gcacggtggt ggtgttaggc gtcactgggc
     3001 aaaactttgc cgcaggcatg agcggcggtg tggcttatgt gtatgatgaa gacggccagt
     3061 ttgccaaacg ttgtaatatg ggcatggttt cattagaaaa agtccagcat atcagccaag
     3121 taagcgcaga tgccatacat cacttgggca cccctgacga aacgatattg cttggcctac
     3181 tggctaaaca cattgcttat accaatagtg aacgtgcaaa aatcattgtg caatattggg
     3241 atgagcatcg cgaaaaattt gtaaaagtga tgccaaatga atacaagcgc gcgttaatag
     3301 agctcgctga agataaagtg ctggaggccg tataatgggt aaagtaactg gatttataga
     3361 gtataaccgc ctctctgagg caaacttgcc agtgccagag cgcgttaaaa attataaaga
     3421 gtttgtttta catttaacgg acgagcaagc ccaaacccaa ggcgcgcgct gtatggattg
     3481 tggcattcca ttttgcacta cgggctgccc aatcaacaat attattcctg attggaatga
     3541 tttggtgtat caccaagatt ggcgcagtgc cattgatgta ttgcactcaa ccaataattt
     3601 tcctgagttc acaggccgca tctgccctgc accgtgcgag gctgcctgta ctttaaatat
     3661 caatgatgat gccgttggta ttaaatcgat tgaacatgcg attattgata aggcttggga
     3721 aaataattgg gtcacaccgc aaatcaaccc caataaaaca ggtaaaaaag tcgcagttgt
     3781 tggctctggc cctgccggtt tagccgcggc acagcaatta gcgcgcgcgg gtcatgctgt
     3841 ggtagtgttg gagaaaaata gccgcattgg tggcttgtta cgttacggca ttccagattt
     3901 taaaatggaa aaatcgcaca ttgaccgccg catggcacaa atgactgccg aaggcgtaga
     3961 gtttcgcgtc aatcagcacg taggtgacaa tgtgagtgct gacgacttgg tggcagaatt
     4021 tgatgcggta ttattggccg caggtgcaga acagcctcgt gatttacccg ttaaagggcg
     4081 tgagctagat ggcgtacatt ttgcgatgga ttttttaacg ccgcaaaata aagtgaatgc
     4141 tggggatgtg attcctaatc agattaaagc cacaggcaag cacgtcatcg tgattggcgg
     4201 tggtgatact gggtctgact gcgtgggcac tagcaaccgt catggcgcgg caagtattac
     4261 acagtttgaa ctgatgccac agccgcctga aaaagaaaat agagcccttg tttggcctaa
     4321 ttggccgctc aaaatgcgca cttcaagctc gcatgaagaa ggcgcaaacc gtgattggtc
     4381 aatcaccacc aaagaactga tgggtgaaaa tggtaaagtg gaagcactta aaggcgcgcg
     4441 tgtggcgtgg aaagacggca aaatgactga aatagctggc tcagaattta cgattcctgc
     4501 cgatttggtg tttttagcca tgggttttgt gtcacctgtg gaaaaattgc tagcagtatt
     4561 tggtgtagaa aaagaccaac gcggtaacgc aaaagcaacg actgaaggtg ctacaagtta
     4621 ccaaaccagc aaagctaaag tatttgccgc aggtgatgtg cgtcgaggtc aatcgttagt
     4681 ggtatgggca attcgcgaag ggcgccaagc cgcgcaagca atcgatgcat ttttaatggg
     4741 cgcatcggta ttgccgcgct aattaccgtt aattctagta aaatagctgg ggatgcgcat
     4801 gacgtgcatc cccatttttt tgagtaagct catgacaaca ttacaaaacg atacttttct
     4861 tcgcgctttg atgcgccagc caaccgatta cacgcctgtg tggatgatgc gccaagcggg
     4921 gcgttatttg ccagagtatc gtgaaagccg caaaacagca gggagttttt tgcaactgtg
     4981 caaaaatgcg caatttgcaa cggatgtgac cttacagccg ctagaccgct acccactgct
     5041 tgatgcggcg attttgtttt cagatattct taccgtacca gatgccatgg gcttaggcct
     5101 gtattttgaa gaaggcgaag gcccaaaatt tgagcgcacc ttgcagctag aagccgatat
     5161 tcaaaaatta gtggtgccag acatggctaa actgcaatat gtgtttgatg cggtttctag
     5221 catccgcaaa gccttaaatg gccgcgtgcc attaattggt ttttcaggca gcccttggac
     5281 actagccact tacatggtag agggtaaagg tggtaccgac tttttaacca ctaaaaaaat
     5341 ggcctatgcg cgcccagatt tgctacacca tatcttggaa acgactgcac aaacagttat
     5401 tcaatatctc aatgcgcaga ttgccgcagg cgcacaagcc gtgatgattt ttgactcgtg
     5461 gggcggtgcg ctttcacacc atgcttatac tgaattttcg ctcaattaca tgcaaaaaat
     5521 tgtggctgga ctcactaaaa atgcagaagg caaaaaagtt ccgagcattg tgtttactaa
     5581 aggtggcgca ttatggcttg aagcacaagc cgacattggc gcagatgcgc tgggcttaga
     5641 ttggacagtg gacattggta gcgcacgaca acgtgtgggc gataaagtcg ccctgcaagg
     5701 caatttagac cctgcaatat tgttatcaac cccgcaggcc attgaaagag aagttgcgag
     5761 cgtgctggca agctacggca aaggccatgg ccatgtgttt aatttggggc atggtattac
     5821 gcaatggacg ccaccagaaa acgcagcatg tatgctaaac gcggtcagag agttgagcag
     5881 acagtatcac gcctaaatta gtctgaaata cacatgcaag tttcagcgaa gactaacctt
     5941 taggttatgt cggagctaaa atgcggcggc aatgttagaa gcagttagaa agcatagtca
     6001 gcaatacaag gtgaattaaa ctcaagaagt gagtttagag aagatgccct ggggtatcag
     6061 taataaaaaa gcccgaaaat ttcgggcttt tttatggttt ttatcttatt ttaaagacat
     6121 gcgtttcatc gtaccgtctt tatcaatgaa cttgtgtttt aatgcaccag caatatgcag
     6181 ggcaatcaca actaaaaaca cgaacccaat aatttcgtgc acttctttga agagttcacg
     6241 tatgtcttta ttgtctaagc cagcaataaa gtcaatgcca aaccacatga ggccgtattt
     6301 actgttgatg gccatgatga ggccgctcaa aggcacggca aacattagca gatacaatac
     6361 atgatgcgtg ccagtagcaa gtttgcgttc ccaggccttg tatgaggtga gcatcgccgg
     6421 tgggcgatgg gtaatacgcc atactagacg taaagcgatt aaagcaagca aagtgacgcc
     6481 aattgattta tgcaggttaa agtaaaacgt tctggggctg gcttcttcag caagattcca
     6541 agtatagagg cctaagtcaa ataaatcgaa gcccaattgt tttggtgcat ctttaggtaa
     6601 ttcactcatg aaccagccga gtgcaaacat gccaaaaaca acaagcgcga tgagccagtg
     6661 tagaatgaca gctacttttg tataacgtgt tgaaacttga ttcattgtag tatcctgatt
     6721 gaaataaaca tttactgtga cagcggtcac agcaaatgat tgcataaaag cgcaccattt
     6781 taatcggtaa aaacttaata aggaatattt catgcgaatt ctaatgataa tcatgagtat
     6841 gttggccttt gttggctgtg cgcattcaca acctaaccaa actgcgtcgg taaaccatga
     6901 tgattttgcg atagaaaatg ttggtgatgg tatttacgtg catcacggcg cgcatcttga
     6961 tattgataca ggttaccaag gcgatatttg taatattagt tttgttgtgg gaagccgcgg
     7021 tgttgccgtg attgacactg gcggcagcct caaagtgggc cagcagttgc gcgaagcaat
     7081 acgcaaaatc acgccattgc ccgtgcttta tgtcattaat acccatgtgc atccagacca
     7141 tatatacggc aacgccgctt ttttaaccga taaaccgcaa tttatcggcc atgaaaaatt
     7201 agccaatgcc atggccacgc gtgaggagca atatgcaaaa ctcaatgtgc aattgttagg
     7261 agaggatgcc aaaggcagtg agctcgtaaa gccaaccatg gcggttaaaa ccacgctgga
     7321 aattgatttg ggtgatcgtc aattacggct gacagcgcac ccagtggcgc ataccaatac
     7381 cgatgtttcg ctggtagata gcaaaaccag caccttattt gcgggggatt tattatttat
     7441 tgaccgcaca ccagtgattg aaagcgatat tgaaggcttg attgcagagc tagaaaagtt
     7501 taaggcaggc cagtttaaac aagtagtgcc aggccatggg ccggtggcga aagactggct
     7561 tgccgcgatt aacaacgccg agcgttattt aaatgtattg ctggcggata ttcgcgccag
     7621 cattaaaaaa ggtgaaagca tggaatctgc catgaacacc gcagccgcga gtgaaaaaga
     7681 aaaatgggtg ctatttgaca ttgccaacag acgcaacgtg aatattctct accccgcgct
     7741 agagtgggaa taataccaac caacattagg cattattcac gcatttttac acgcaacctt
     7801 ttttgatgct ctgtaagcct ttatttataa ggctcgcaga gcatttattt tttctcgaga
     7861 gaaattaagc gtgttttaaa gagttatttg ttgcgattat caagactaaa agcggtattt
     7921 gaattgacat aaagcgtgat taacccgcct gcaattgcta aatattgaag gactggcaaa
     7981 tggagtaaat aggaaataat cactgcataa attgccatca ataatgcaaa aaatttcgtt
     8041 ttaaaaccta agaataaagc caaaccaccg accaattgca ctaaaatgat taaaggtgca
     8101 aaaattcctg gcaagccaag gctacctaag cccgcttgat attgttggta accatcgggc
     8161 gtgttaaaaa agccaacgat taaaataatg acttgtaata aaaaaacttg agcaagtaaa
     8221 actctgccca gtgtgcctaa atatttttgc ataaatgact ttcaaattga caatcaacag
     8281 agcctgcata atgcaccgaa caatgcaatt tcgcaagtgt atctaaaatg aaaaactggc
     8341 aactaatcac cacagtttcg ctgtcagcga gggctacaga gccaaacgcg gcctcaaaaa
     8401 tcagtatttt tcaagtttcg tggcaagcgg cgcaacacga actgcaagca gtaagaacgc
     8461 ccgtttttgt ggtggagcag ttagtcagcc cagattttga atgggatgaa attgatgcgc
     8521 aagccgtgca tttattggtc atgcatcatg cgcaacccat tgcctgcctg cgcatcattg
     8581 cgtaccaaaa aattggtcgc atggcggtgt taaaacaatg gaggaacaaa ggcttgggca
     8641 aggcgttgct ggaggaggcg gttcgtattt gccgcgcaca gggtagcgct tctgttcgct
     8701 tatcagccca aacgcatgcg gtacagtttt accaaaaggc gggctttaag caaacaagtg
     8761 aggaatacac ggatgtgcat attccacacg tggatatgca actggattta taaattgttt
     8821 ggtaaaataa cgctttatgt tttaatccat tttcaatcta attttttaac aacgcaaaga
     8881 gaaaatcatg tcaaaaatca tttttaaagc gggcgaagcc accgtatatt cagaaggtaa
     8941 agatgtaaca gccgcaatgc cagaaatttt aattggcgcg gttgacggcc cggtaggcca
     9001 ggcgtttgca aacctaatgg cgcaaagtaa aggccacact gcgatgtttg ccgtacgcga
     9061 cattaaccaa cttgtgcgcc ctgtgtgcat gacggtgcca aaagtcacct taaaaggcag
     9121 cacggacgtg ggcttgtttg gtggtgtggt gcaagcggct accgccgatg cgattttaga
     9181 ttgcgtgatt gaaggcatta tcccgaaaga tcaggccaat gatttatgta ttattagctt
     9241 agtgtggata gacccaggtt gtattccact tgaaaaagaa ggcaaacttg ataaagccga
     9301 catgtacaaa aacaattatg atgcgactaa attagccatt aaacgtgcgc taaatgacga
     9361 gcctagtatt gatgaactga ttgcaaaccg caaaaaaatc aaacactgca tgtgggaaga
     9421 aagctggggc gaaatttaat tttcacatta atttacgcca tacatcgcaa tatagtgtta
     9481 cataaatcgt gttagttttg ttatgataaa aacatgacat gaaattcgga gggtgtatgg
     9541 ctgaacataa tgtatgtaaa gaagcctttg accggctttg tgatgaagta aacaccgaca
     9601 aaaagtcggc aattaaccca gatgactatt ggctgtttga gttgggcttt cggtctgcca
     9661 ttgaagaact gctgtcgata gctgatgcag gtgagcaggc gagaaaattt gtctcaccgc
     9721 gctttcaaat gctggcagac aaaatactca attccagact gcattaaaca aaaaagcggc
     9781 aagttgccgc tttttttacg ccatttactt agctggcgct attgagtacg ttttgcaaaa
     9841 gcatataaaa taaagccgat tacaacgaca actccaacaa agatgaaaac gccttgccat
     9901 ccatcaaaaa gcatcattga ctccttttta gcttatttaa gcgcgctggg ttaataaaat
     9961 gcacgaccag caacgaataa taatattttt gacacaggca acacgttgat ataagtcaat
    10021 aaacttaatt tattagaaca ggatttcaac catgccttct atgtaccaac acaccattcc
    10081 accattaaaa cgcgctttgc gtaaccttgc cgccatcctt aaaaaaggcg ccgcttatgc
    10141 tgaagcgaaa aatatcgagc cgggtgtgct gttgaatgca cggctttttc ctgatatgta
    10201 tccgctggtt cgccaggtgc aaattgccac ggacatgagt aaaggcgcag ctgcgcgctt
    10261 ggcaaatgtg gaaattccaa agtatgaaga caatgaaacc acctttgatg cgctgcaggc
    10321 gcgcattgcc aaaaccattg cgtttattga cagcattcag ccagcacaac tcgaaggcag
    10381 cgaatcacgc gaggtggtga ttagtgtacg caagattgaa ctcaaattta cggggctaga
    10441 ttacttgttg aaatgggtga accccaatgt gtattttcat gtgaccacgg cgtacaacat
    10501 tttgcgccac aacggtgttg agctgggcaa gtcagatttt cttgggccca gaaaagattg
    10561 attttttact tcaacccttg tttcttagcg gctcaagcaa cgccgataag tcgttatggt
    10621 caatctcttg catgagctgc aataagcgcc ccaagtcacc ctttggaaaa ccttcgcgcg
    10681 caaaccagtt taggtaatgg ccgggcaaat cggcaatcaa acggccttta tacttgccat
    10741 aaggcatggc cagcgtcacc aaacgttgta aatcatcgga gttcatatgg ggtagaagtt
    10801 tagcatttgg cagccaaaaa tgttgtgtgc aaaacacacc ttttacttgc taataacgcc
    10861 cgagaaaatt tctctgccct gtgagccttt atttataagg ctcacagggc atcaaaaaag
    10921 gtttgttgta attgagccgt taaaaaagcg tttattttgc attggccagc tgtaatccac
    10981 aatcttgcga gacttgttgc tcaagtgcgt taaacgtatt tcccgcccct gtggcactgg
    11041 catatggatt tgagcgcaag taggtttggt attcaacttg gcgcgcgcta accaccttgg
    11101 cgagtgcatc tatcttacag gtacacgcct cagtgacatt tgttgccttg gcgcgcgcac
    11161 tgcattgcgc aatttcattg tcacgaaact cttttggcca agcaagcgaa ttggtttgat
    11221 tttgcgccaa caccgcgcgc gattgcgata acgtggcata gagcatttca atgctcgcac
    11281 gcagtttgcc tagctcggct gtttgtaaga tatcttgatg agataccgcc aatgtgcctg
    11341 aatcaacctc aaattgcgcg cttactgtgg tggattcagg taaagcgtag ccttgtgaaa
    11401 ataatataat gcgttttgcg gggtcggcct caagcacttt attgccttca gctaaatcgt
    11461 ccgcaggcaa attaaaagtg gcttgcgcgc gacacgccac accttctgcc agcgccattg
    11521 tgagcgtgat tttgccatcg tctaccgcgt gtttaaaagc cggcggcacg cgattaagca
    11581 aataattcag ctcattttgg cgcatttttt caagcgcgtt ggcatctagg catggggttg
    11641 cctgagcgct ggcagaaaac caaaatgcca acaaacacgt agtgtagagc gcagtatttc
    11701 gcatcacaaa cgccctattt aagcgcaggt tcaatcagtt ttaaaatggc ggcatcgtct
    11761 ggttcaacgc cacttttaaa gctttgtaca gtttttccat cggcagaaat cacatactta
    11821 tgaaaattcc actgcggttc ggttccactg gctttaatga gctgtttgta aaaagggttt
    11881 gcgttttcgc ccttaacaga accttttgac accatgggaa acttcacgcc ataggttaat
    11941 ttacaaaaac tgccaatttc agaatcgctg gtgagttctt gtttaaaatc gtttgacgga
    12001 aagcctatca ccaataagcc gttgtcttta taacggctgt agaggccctc cagcttttca
    12061 aattgtggcg taaagccgca tttgctggcg gtgttcacca ccaaaatagg tttgccacga
    12121 taatcgcaca ggttaatggt gttgccctgc aaagttttaa actggtggtt taataaagtc
    12181 gggcaatcgg cagcaaatgc atttgtggct aacatgaaca atactcctat taaaaaacgc
    12241 atggcaaact cctttattta ttgtagccat atgagttggg gcgcttgcta aagttcaaca
    12301 tcgcccttgt gctaatttga gattattttt aatcaatatt gcactaaaaa caatatttat
    12361 tgttacaata aattcatgtc agaaaatcac ccaatacact taagcaaaat tgccccgcgc
    12421 aaaaataagc ccggcggtgg ccgtaaggtt ggcacaggca aatttggtga ggcaactgcg
    12481 gtgatgcgtg tgccggcaag ccaaacgctg ataattaaag attttttggc ggcgtatcag
    12541 caaaaaatgc gcgccacaaa tttagatgcc gtgcgtgaat ttgaatggcc cgctgatgaa
    12601 gtaaaagccg ttgatttgcc gctttatagc tcaaaagtgt ctgctggtct gccctcacct
    12661 gcggaagaac atgttgaaaa acggctagac cctaacgaat ttttaattga ccaaaaagac
    12721 gccacttttt ttgtcaccat tcaaggcatg tcgatggtgg atgttggttt attgccgggc
    12781 gataaagcgg tggtggatcg ctcaaaattg ccgtcgattg gtgatattgt gctggcggtg
    12841 cttgatggcg aatttaccat taaaaccctc agccgaagta agcatggcaa tgtgcgcttg
    12901 ttgcccgcta atgctacggg ggcttattcg ccgattgaaa ttactgaaaa tatgaatttt
    12961 gagatttggg gtgtggtcac gggttcattc aggcgcttta aatagcaaac cccaccattc
    13021 aaaaaaccta gcaaaagcta ggttttttga atggtgtact tttaagtgct taacgttgat
    13081 tacttgttca ttacgtacat tgtcacttca aagccaaaac gcatttcagt cgctgcaggt
    13141 gttgtccaca taataatctc cattttttaa ttttctgaac cgacaacatg tcgtgttcga
    13201 gtgagttcat tatgcgcttt ggcaaaaatt acacactatt gctgttcatg aatcacgcct
    13261 acgtaaaatc atgagtcacg cctatattgc acccttcaat tattgtgcag gctttgccaa
    13321 ccaatacaaa ggcgcatctt ggcttgggta aaacgcctcc atatcatgtc gctttaaatt
    13381 ctcttgcgct tcatccaatg aaatattttg tgtgttggcg gctgcctgtg ccagcggttg
    13441 cgttgctttt acccacgcag ggttttcagt aaagccttgg tcgttcatgc agttttgtaa
    13501 aacgcgcgcg cgtctgccgg caatttctag tttttgatac tcaacaacgg cggtgagatt
    13561 ggccaccgat ttatcggcaa tgccgctaca agagttcagc gccaagctca tagcgctggg
    13621 ttcagccttt ggttgatctg ctgaaaacca atcaaacgta ttggccaccc aatacagcat
    13681 tgggcacagc acggtgaggg ccgctaggct ataaacgaat ttcatgttca tcattaatca
    13741 acttttaatt caagttttaa caagtagcac attgctgcaa agcgcccagt gcatcatcca
    13801 gagattgtaa tgtgtctatg ctaacagttt taagcacagc acgaatcgta ttcacctccg
    13861 ccgcgttgcc ctgctggtgc gccatcaaag ccagtgcctg caaactggcg gggtgattgg
    13921 ggtgcagcgc gagcgcttta ttaaagtgtt tcagcgcatt ggttgagtcg ttttggtttt
    13981 gcgctgctgc gcctaagtaa aaccaagctt ctgcgttatt gggttcagca ttgcgccagt
    14041 tgctggcgat tttttgcacg tccgcccaac ggttattttg aaaaggcagc acaatttgca
    14101 taaaaaatgg ccgaccatct tcaggcgcat cccaaaaagg caatgcgtgt gccgcatcaa
    14161 actgcatggc gggttgttgc aagagcgccc tcacccattt caccggcatg ttataaaaat
    14221 aggcattgcg accggggctt ttaaaggtgc taatgcctat taaattgccg ctgtaatcaa
    14281 acacagggct accgctggcg ccaatggcaa aagccgcttt ggtgcgtatc acttcgccat
    14341 catccattgg gtagagtgct ttaatggcgc ttagctccac atacggcgcg ggtgagtcgc
    14401 tgggcattga aatggaaatc acaggctgtt catattgcat ggcatcggat tcggctattt
    14461 tcacgggttt aaaatcggcc cattcaaatt ttaaaatgca caaatcgtgc ttccaatcgg
    14521 cctgcatgct cacaggcgcg tattccacac cccacttgct cacgctaatg ccattggcgt
    14581 tatccaccac atggcaattg gtgacaatat ggtcttttga aacggccacg cccgtgccaa
    14641 agccatgccc gccagacttg gtgtttacag ccaccttcac caatgattct ttaagctgat
    14701 aaataaacgc atcggttggt tcggcgggtg gcgcagcact ggcaaacggc actaccaagc
    14761 ctagcaagta aagtaatttt gcggttttca ttggtgttgt tccatgcaaa aaaacattga
    14821 ccacacaaac accattttgt ttaaaacacg atgatttatt ttaaataaat ccagatattg
    14881 aaaagaatga attatcacaa agtttacaca ttatatta
//
LOCUS       DAOQSI010000032        14689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_3163_length_14689_cov_5.478065, whole genome shotgun sequence.
ACCESSION   DAOQSI010000032 DAOQSI010000000
VERSION     DAOQSI010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 14689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14689
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3163_length_14689_cov_5.478065"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="PKL42_03565"
     CDS             complement(<1..901)
                     /locus_tag="PKL42_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNU66182.1"
                     /translation="MAASIKATKEVIYSWEGKDKKGKAVKGEMKASGDSFVSATLRRQ
                     GITVTKIKKQSSFKSKGSVSDKDVTLFTRQLATMMKSGVPLLQTFDIVGKGHANPAVG
                     KLLLDLKSDIETGSSLSAAFRKYPLYFDALYCNLVGAGEQAGILDTLLDRLASYKEKI
                     QAIKSKIKSALFYPISIIVVAFVIVAIIMIFVIPAFKQLFEGFGADLPGPTLVVMAIS
                     GFFVEWWWAIFGSIGFGLWFFFYTWKRSISMQATMDRLMLKIPIFGPLVRKATIARWS
                     RTLSTMFSAGVPLVEALDSVAGAC"
     gene            complement(966..2684)
                     /gene="pilB"
                     /locus_tag="PKL42_03570"
     CDS             complement(966..2684)
                     /gene="pilB"
                     /locus_tag="PKL42_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HNU66183.1"
                     /translation="MSTVAPTVKLSGIARTLIQEKLLTEEEATAIQTKANAANTPFLT
                     HLIASKKLSAAAVAASSAKAFGFPYFNLDAYNIDYLPSKKIDAQLMLSHRVLAIQARN
                     NVLFVAISDPTNLHALDSVQFQMGMALSPVIVEDDKLGRLIDKVIEASDASMKSLHTD
                     DEFDLGEDIGQTVEEEAQAQEVDDAPVVKFLNKILIDAINMGASDLHFEPYEKFYRIR
                     YRVDGMLREITQPPLAIKEKLASRIKIISSLDISEKRIPQDGRMKLVLSKTRTIDFRV
                     STLPLIHGEKIVMRILDPSSATLGIEALGYEPIQKERLLNAVSRPYGLVLVTGPTGSG
                     KTVSLYTCLNILNNPGVNISTAEDPCEIPLPGINQVNVNDKQGLTFAAALKSFLRQDP
                     DIIMIGEIRDLETADMAIKAASTGHMVLSTLHTNDAPSTLTRLLNMGVAPFNIASAVS
                     LITAQRLARRLCKSCKVPIDVPKEALLGVGFREEDFNESWQLYGAKEGGCDMCNDGYK
                     GRVGIYQVMPITDEISRIIMSNGNAHDIKDQAEREGVNDLRRSGILKVMQGLTSIEEV
                     EGCTNE"
     gene            complement(3029..4267)
                     /gene="icd"
                     /locus_tag="PKL42_03575"
     CDS             complement(3029..4267)
                     /gene="icd"
                     /locus_tag="PKL42_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNU66184.1"
                     /translation="MASKIVVPATGSKITVNKDNSLNVPNNPIIPFIEGDGIGADITP
                     PMIKVVDAAVHKAYGGTRQIAWMEVYAGEKATKVYGENDWLPQETMKAVRDYVVSIKG
                     PLTTPVGGGIRSLNVALRQELDLYVCLRPVQYFSGVPSPVKHPEKTDMVIFRENTEDI
                     YAGIEWAAGTDEVEKVIQFLSDMGMRKKIRFPESSAIGIKPVSKEGSQRLVRKAIQYA
                     IDNKRKSVTIAHKGNIMKFTEGGFRDWGYEIAKKEFGAVEIDGGPWCKLPNGILIKDC
                     IADAFLQEILLHPQDYDVVATLNLNGDYMSDALAAQVGGIGIAPGANLSDTVAMFEAT
                     HGTAPKIAGKDLANPSSIILSAEMMLRHLGWIEAAELVIQGVAKAIIAKRVTGDFSNE
                     MENATQVGTAQFGEEIIANM"
     gene            4316..4846
                     /locus_tag="PKL42_03580"
     CDS             4316..4846
                     /locus_tag="PKL42_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit pseudouridine synthase E"
                     /protein_id="HNU66185.1"
                     /translation="MIILFNKPFNVVCQFSAHEKHATLKDFIPIPNVYAAGRLDTDSE
                     GLLILTDDGALQHRLSDPKFKEFKTYWVQVEGAPTENDLAPLKAGVDLGDFVTKPAQV
                     KLIEAPQNLWGRHPPIRERKAIPTTWLEIKISEGKNRQVRRMTAKVGFPTLRLIRYAV
                     GAYTLEGLAVGEYKVQ"
     gene            5129..6217
                     /gene="aroC"
                     /locus_tag="PKL42_03585"
     CDS             5129..6217
                     /gene="aroC"
                     /locus_tag="PKL42_03585"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNU66186.1"
                     /translation="MSGNTIGKLFTFTSFGESHGAAIGGVVDGCPPGLSLCAEDLQLD
                     LDRRKPGTSRHVTQRQEADLVEILSGVFEGKTTGAPIGLLIRNTDQRSQDYSKIMDTF
                     RPGHADYTYTQKYGVRDYRGGGRSSARETAVRVAAGAIAKKWLKERFGVAVRGYMSQL
                     GEIDIPFETWDCVNENPFFSPNSKVLPQLEAYLDTIRAERDSVGAKITVVAEGVPVGW
                     GEPIFDRLDAEIAYAMMSINAVKGVEIGAGFAAIHQRGSEHSDEMTPQGFVTNHAGGI
                     LGGISTGQEIRVNVALKPTSSIPQERRSIDKNGNAVTMQTTGRHDPCVGVRATPIAEA
                     MLALVLMDHALRHRAQNADVTSSTPILK"
     gene            6329..6565
                     /locus_tag="PKL42_03590"
     CDS             6329..6565
                     /locus_tag="PKL42_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HNU66187.1"
                     /translation="MSIEHHDLVHELPEHREKIHSMKMSNAHFAKLFDEYHVVTNEVE
                     QIEKSGVPVSDVDFENQKKKRAQLKDQLYSMLIS"
     gene            6748..7953
                     /locus_tag="PKL42_03595"
     CDS             6748..7953
                     /locus_tag="PKL42_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNU66188.1"
                     /translation="MSHALHFNLSRFYFIYYFFVGAFVPYWGLYLAAEKFSPADIGIL
                     MSLFQISRIFAPNFWGWLADHTAKRVVWIKLTAVLGLLGFVAVFWAHGFWAIFLVMAA
                     LSLFTSSTLPLSESLTLAHLATTNGHYSRIRMWGSLGFIVASVILGYVTEAFGIRSLL
                     WFLLAVQIVLFALTFKLPEAKVAPHAHDEFSIWQVVRQPNVIALLLGCALMVTAHGVL
                     YNFYSIYLVQHGYSKSTIGLLWSVGVICEIGIFMWMPKIMARYSLKTILLTSLALAVL
                     RFSLIGVAIDSLFMIVLAQTLHAATFGSFHAASVEVVTQFFNGRHQAKGQAIYNSVAY
                     GVGGTVGGVAGGFGLQYLGQLANWYGWTYSSGQITFMLAAIFPLIGFLVIGLGLKLTP
                     QNQTQGMFR"
     gene            7950..8606
                     /locus_tag="PKL42_03600"
     CDS             7950..8606
                     /locus_tag="PKL42_03600"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HNU66189.1"
                     /translation="MKVIIKFCFCFCFILLTANLAQAATQVNIVGLFSNKAVVIINGG
                     KPKTLSVGQTSEGVKLLAADSQTATLQIEGKTQQLGMGQAASVGGNSANAGVASVTLY
                     ADTAGHFISECYVNGAPLKFLLDTGATTVALNSGDAKFAKIDYKRGEPIQVSTANGVV
                     TAYRVTIATLKIGALTLNQVEASVLEGGSPSVVLLGMSALNRLDMKRQDIALTLSKKY
                     "
     gene            complement(8772..8978)
                     /locus_tag="PKL42_03605"
     CDS             complement(8772..8978)
                     /locus_tag="PKL42_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HNU66190.1"
                     /translation="MSVAKVIEIIASSQKSFEDAVSNGVAKAAESLHGITGAWVQDQS
                     VKVKDGKVTEYRVVMKVTFVLKDD"
     gene            complement(9091..9897)
                     /gene="folE2"
                     /locus_tag="PKL42_03610"
     CDS             complement(9091..9897)
                     /gene="folE2"
                     /locus_tag="PKL42_03610"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HNU66191.1"
                     /translation="MTKSTHPAAIEDVQNTVDTRHIAIDKVGIKAIRHPVIVKDRSGG
                     EQHTVAMFNMYVHLPQQFKGTHMSRFVEILNMNEHAISIESFETILREMVKRLEAESG
                     HVEMTFPYFVNKAAPISGVKSLLDYEVTFIGAIEHGKFDITVKVLVPVTSLCPCSKKI
                     SDYGAHNQRSHVTVTALINDFIWIEDIIDLVENQASCQLYGLLKRPDEKFVTERAYDN
                     PKFVEDMVRDVAAQLNKEKRIDAYTVESENFESIHNHSAYALIESDKRVK"
     gene            complement(9950..11818)
                     /gene="dxs"
                     /locus_tag="PKL42_03615"
     CDS             complement(9950..11818)
                     /gene="dxs"
                     /locus_tag="PKL42_03615"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNU66192.1"
                     /translation="MTSFPRQPILDAIDFPAQLRQLERAQLPQLAAELRAFIIDSVAK
                     TGGHLASNLGVVELTIALHYVFNTPDDRLVWDVGHQTYPHKILTGRREAMQHLRKPKG
                     IAGFPRRAESEFDTFGTGHSSTSISAALGMAVAAQKAGLERRAVAIIGDGALTGGMAF
                     EALNNAGNMDANLLVVLNDNDMSISDNVGGLNRYLAKLLASRFYGTIRKSGKKVLSGM
                     PHMQEFAKRAEEHVKGMVLPGTLFEELGFNYIGPIDGHDLEALITSLQNIKQLKGAQF
                     LHIVTQKGKGFEPAEDNPNKFHGVGKFDPFNGDAITSVSKKTYTQVFGEWLVDMADAD
                     QRLIGITPAMCDGSGLNAFAEKYPQRYFDVGIAEQHALTFAAGMACDGLKPVVAIYST
                     FLQRAYDQLIHDIALQNLPVLFAIDRAGLVGADGPTHAGSFDLSFLRCIPNIIIMAPS
                     DENECRQMLTTGFQHHGPAAVRYPRGSGTGVATEAALNAIAIGKGEIKLSSHKPKQKV
                     AILAFGSMLTPALEAAEKIDATVANMRFVKPLDTALIAELAKNHDLIVTIEENAVMGG
                     AGSAVMEALQAMRIVTPILCLGLPDTFIEHGDHATMLAACGLDASGIIAAIEQIIP"
     gene            complement(11896..12792)
                     /locus_tag="PKL42_03620"
     CDS             complement(11896..12792)
                     /locus_tag="PKL42_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNU66193.1"
                     /translation="MQKPNAGAFQDWAQQQQARIETVLARTLPAADVAPQTLHSAMRY
                     SALGGGKRVRALLCYAAAELCNTELKIADAAASAVELIHAYSLVHDDMPCMDDDDLRR
                     GKPSCHKQYDDATALLVGDALQSLAFEVLAAPQLCANAHQQVQLLHMLAIASGSTGMA
                     GGQAIDLGAVGKPLTQTQLESMHQLKTGALIQASVLLGAINGSTAQIKAVSEYARNIG
                     LAFQVVDDILDVEADTSTLGKTAGKDAMSQKPTYVTLLGLVEAKAYATQLYENAILSL
                     VTFGEEARRLRELASFITQRQF"
     gene            complement(12823..13089)
                     /locus_tag="PKL42_03625"
     CDS             complement(12823..13089)
                     /locus_tag="PKL42_03625"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNU66194.1"
                     /translation="MNSAKPKSTRILATQEVAPDNFEHALTDLEKIVAQMESGQMSLE
                     ASLAAYQRGDALLQYCQKSLAEVEQQVQILNARNQLNPFQPNQE"
     gene            complement(13341..14012)
                     /locus_tag="PKL42_03630"
     CDS             complement(13341..14012)
                     /locus_tag="PKL42_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNU66195.1"
                     /translation="MNHQPPLELSQSGAQITASVILMHGLGADGHDFAPIVSKLNLPH
                     IRFILPHAPEMPVTRNGGFIMPAWYDLYGQPNNQTGYPIEDEAGIQVSQHYIHSLIAH
                     EVARGISTKRIMLAGFSQGGAIALYTALRYPQPLAGVLALSTYLPLKSKLAVEANFAN
                     AHCPIFMAHGVADDIISLDMCKNSRSVLLDNHYPVDWHEYAMAHSVCLEEIDDMRAFL
                     IAKLP"
     gene            14104..>14689
                     /locus_tag="PKL42_03635"
     CDS             14104..>14689
                     /locus_tag="PKL42_03635"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNU66196.1"
                     /translation="MRIFTLISQHAVRFALGAAVLVLLLLNATGYVHLSAIQKLELFT
                     YDTRLNMLLPKGVDERIVIIDIDEKSLQAQGRWPWGRNKLADLVNILFDDYHIQVLGF
                     DMVFAEKDESSGLKHLEAIQSQYLSTDPELGQALGQAVETLRPTLDFDALFANSLKNR
                     NVVLGYVFSHDVASVGSGATSCENTYPSTTFRFFR"
BASE COUNT         3792 a         3236 c         3533 g         4128 t
ORIGIN      
        1 cacaagcgcc agccacagaa tctaaagcct ctactaaagg tactcctgca gagaacatgg
       61 tggataatgt gcgagaccat ctggcaatgg tggctttgcg tacaagtgga ccaaagattg
      121 gaattttaag cattaaccga tccatcgtag cctgcatgct gatagagcgc ttccaagtgt
      181 agaagaaaaa ccaaaggcca aacccaatcg agccaaatat tgcccaccac cactctacaa
      241 agaagccaga aatggccatc acaacgagcg ttggaccagg taaatcagcg ccaaagccct
      301 caaacaattg tttgaaagcc gggatgacga atatcataat gatggcaaca atcacaaacg
      361 ccactacgat aattgaaatt gggtaaaaca aagcagattt aatttttgat ttaattgcct
      421 gaattttttc tttgtagctt gcaaggcggt caagtagggt gtctaaaatg cccgcttgct
      481 caccagcacc cactaagttg caataaagag catcgaagta aagaggatat ttacggaaag
      541 cggcgcttag gctactacca gtttcaatgt ctgattttag gtcaagtaat aatttaccta
      601 ccgcagggtt ggcatgacct ttacccacaa tatcaaaagt ttgcagtaaa ggtacgccag
      661 atttcatcat ggtggcaagt tggcgggtaa atagtgtcac atctttatct gaaacactgc
      721 ctttgctttt gaaactgctt tgttttttga ttttagtgac ggtaatgcct tggcgacgaa
      781 gtgttgcgct gacaaacgag tcacctgatg ctttcatttc gcctttgaca gctttgcctt
      841 ttttgtcttt accttcccaa ctgtaaataa cttccttcgt ggctttaatg cttgctgcca
      901 tagtaatccc ctgtatgtgt ttttgtgcct agcatttaaa tgcagttggc tgatcaaaca
      961 aatgtctatt cattggtgca accttctacc tcttcgatag aggtgaggcc ttgcatcact
     1021 ttaagtatgc ctgagcgacg taaatcgttg acaccttcgc gttcagcctg atctttaata
     1081 tcatgcgcgt taccgttgct catgatgata cggctaattt cgtctgtaat cggcatcacc
     1141 tgatagatac caacacggcc tttatagccg tcattacaca tgtcgcagcc gccttcctta
     1201 gcaccgtaga gttgccatga ctcattaaag tcttcttctc taaagcctac gccaagtaaa
     1261 gcttctttag gcacgtcaat cggcacttta cagcttttac ataagcgcct tgccaaccgc
     1321 tgggcagtaa tgagagatac tgcagaagca atattaaagg gcgcaacccc catgttgagt
     1381 agccgagtaa gcgtggaggg ggcatcattg gtatgcaggg tagaaagcac catgtggcca
     1441 gttgatgccg ccttaatagc catgtctgca gtttctaaat cacgaatctc accaatcatg
     1501 atgatgtcgg gatcttgtcg taagaatgat tttagcgctg cggcaaaggt gagcccttgc
     1561 ttatcattca cgttgacttg attgatgcct ggtagtggaa tttcacaggg gtcttccgca
     1621 gtggaaatat tcacaccagg attatttaag atattaagac aggtgtagag cgacacggtt
     1681 ttacccgaac cagttggacc agtgacaagc accaagccat atgggcggct gaccgcattg
     1741 agtaggcgtt ctttttgaat aggctcatag cctagcgctt caataccaag tgttgcgcta
     1801 cttgggtcta aaatacgcat cacgattttt tcgccatgaa tcaacggcaa ggtgcttacc
     1861 cgaaaatcaa tggtgcgagt ttttgacagc accagtttca tgcgtccatc ttgcggaatg
     1921 cgtttttcag aaatatctag gctagagata attttgatac gtgaggccaa tttttcttta
     1981 atggctagag gcggttgcgt gatttctcgc aacatcccgt ctacgcgata gcgaatacgg
     2041 tagaattttt cataaggttc aaaatgtaag tccgacgccc ccatgttaat ggcgtcaatc
     2101 agtattttat taagaaattt aacaacaggc gcatcgtcca cttcttgggc ttgcgcttct
     2161 tcctcaacgg tttgtccaat atcctcgccc aaatcaaatt cgtcatctgt atgcaatgac
     2221 ttcatgctgg cgtcgctggc ttcgatgact ttatcgatta atcgcccgag tttatcgtcc
     2281 tcaacaataa cgggagataa agccatgccc atctgaaact gcacactatc gagtgcatgc
     2341 agatttgtgg ggtcagagat ggcgacaaaa agtacgttat tacgtgcctg aatagccaat
     2401 acacgatgtg ataacatcaa ctgtgcatca atttttttgc tgggcaggta atcgatgtta
     2461 tacgcatcta aattaaagta cggaaagcca aacgctttgg cggaagatgc cgcaacggct
     2521 gcggcactta attttttgct ggcaatcaga tgcgtcagaa atggcgtgtt cgccgcattt
     2581 gctttggttt gaatagccgt agcctcttct tcagtgagca atttctcctg aatcaaggtg
     2641 cgggcgatgc cgcttaactt cactgttggt gctacggtag acatagaaaa atcattaatt
     2701 gcgtgtgaac gctactttca aaaaaaactt tatcaggcta ataatgccct ctagtgtttt
     2761 ttttgtaaag actttatggc tttaaaattg taaaaaattg gtataaatca tacagtaagc
     2821 gcgtaaagct tatgtttttg ttaaaaagcg cacgtgatat cgcgcgtcgt gtatttgagt
     2881 ttggggtatt aggcgcttaa attttgcaaa aaagcgtgaa attgccaaac ttactagaag
     2941 aatacttgag aggtgctctg taagccttta tttaaaaggc ttacagaggc ataaattttt
     3001 ctcgtgagat gaaacgtctt aatttttact acatattagc gatgatttct tcgccaaact
     3061 gtgccgtacc tacttgcgtt gcattttcca tttcatttga aaagtcgccc gtgacacgtt
     3121 tggcaataat ggctttcgct acaccttgaa tgactagttc agccgcttct atccagccta
     3181 agtgacgcaa catcatttca gctgaaagga taatcgagct tgggttggct aaatctttgc
     3241 cggcgatttt gggtgctgtg ccatgtgtag cctcaaacat ggcaacggta tcgcttaagt
     3301 tggcgcccgg cgcaatgcca atgccgccca cctgcgcggc aagcgcatca ctcatgtagt
     3361 cgccatttaa gttaagcgtt gcaaccacgt catagtcttg cggatgcaac agaatttctt
     3421 gtaaaaatgc atcggcaatg cagtctttga tgaggatgcc gttgggcaat ttacaccatg
     3481 ggccgccatc aatttctact gcgccaaatt cttttttggc gatttcataa ccccaatctc
     3541 taaagccgcc ttcggtgaat ttcatgatgt tgcctttatg ggcaatggtc actgattttc
     3601 ttttgttatc aatggcatat tgaatggctt tacgtactaa acgctggctg ccttcttttg
     3661 aaactggctt aatgccaatg gccgatgatt caggaaagcg aattttttta cgcatgccca
     3721 tgtcgcttaa aaattggata actttttcaa cttcgtctgt gcctgctgcc cactcgatgc
     3781 ccgcgtagat gtcttcagta ttttcacgga aaatcaccat gtcggttttt tcaggatgtt
     3841 tgactgggct tggaacgcca gaaaaatact gtactgggcg taaacagaca tataaatcaa
     3901 gctcttggcg caaggccacg tttaatgagc ggatgccacc acccacgggc gtggtgagcg
     3961 ggcctttgat tgaaaccaca taatcgcgta ccgctttcat ggtttcttgc ggtaaccagt
     4021 cattttcgcc atacactttg gtggcttttt cacctgcata cacttccatc caggcgattt
     4081 ggcgcgtgcc gccgtaggct ttatgcacgg ccgcatccac aacttttatc attggcggcg
     4141 taatgtctgc accgatgcca tcgccctcaa taaatgggat aatgggattg tttggcacgt
     4201 tcagcgaatt atctttattt accgtgattt tactgccagt tgcagggaca acaattttag
     4261 aagccatgat ttttccttat tttttaacgc tattgttcac gattgaatgc gcgtcatgat
     4321 aattttattc aacaaacctt ttaacgtcgt atgccagttt agtgcgcatg agaagcacgc
     4381 cacgttaaaa gactttatcc caatcccgaa tgtgtacgct gccgggcgac ttgataccga
     4441 tagcgagggt ttgcttattt taaccgatga tggcgcgttg caacaccgtt taagtgaccc
     4501 aaaattcaaa gaatttaaaa cttattgggt gcaggtcgaa ggtgctccga ctgaaaatga
     4561 tttggcgccc ttaaaagctg gggtggattt gggtgatttt gtgacaaaac cggcacaggt
     4621 caagttgatt gaagcgccgc aaaacttatg ggggcggcat ccacccattc gtgagcgtaa
     4681 agcgattcca accacttggc ttgaaatcaa aatttctgaa ggcaaaaatc ggcaagtacg
     4741 gcgcatgaca gctaaagtgg gctttccaac attgcgtttg attcgctatg ccgttggagc
     4801 ttacacctta gaggggcttg cagtgggtga atataaagtg cagtaaaaca gcatgctaaa
     4861 tcaagccaat taaagcgtaa aatcctacga gaaaaaaata atgccctgca agcctttatt
     4921 tataaggctc gcagagcatc gaaaaaggtt gtggcttatt tagcaataaa aatatagccg
     4981 attcacctgc aaaatgtctg cgtattgtgc gaaattttgc gctgttaaaa caggtagaaa
     5041 attgtgcatg ttgcaacaac atcaaccagg cggtctattg gcgcgcatgt gctaaaatta
     5101 caagctattt caatgctcga gaatcgatat gtctggtaac acgataggca aactatttac
     5161 gtttacatct tttggtgagt cgcacggtgc ggctatcggc ggcgtggtgg acggttgtcc
     5221 accaggatta agtttatgtg cagaagattt gcagctagat ttagaccgac gtaaacctgg
     5281 cacgtcacgc cacgtgacac agcgtcaaga ggctgatttg gtggagattt tatcaggcgt
     5341 gtttgaaggc aaaaccacag gcgcgcccat tggcttattg atacgcaata ctgaccagcg
     5401 tagtcaggat tattcaaaaa tcatggatac ctttcggcct ggccatgcag attacaccta
     5461 cacgcaaaaa tatggcgtgc gtgattatcg gggcggcggc cgctccagtg cgcgcgaaac
     5521 cgccgtgcgt gtggccgcgg gtgcgattgc caaaaagtgg ctcaaagagc gctttggtgt
     5581 ggcggtgcgc ggatatatga gccaattagg tgaaattgac attccatttg aaacatggga
     5641 ttgcgtgaat gaaaatccgt ttttttcacc caattctaag gtattgccgc agttagaagc
     5701 ctacttagat accattcgtg ctgagcgtga ttctgtgggt gcaaaaatca ctgtggtggc
     5761 cgaaggtgtg ccggtcggct ggggtgaacc aatttttgat cggctggatg ccgaaatcgc
     5821 atatgccatg atgagcatta atgccgtcaa aggcgtggaa attggcgcag gctttgcggc
     5881 gattcatcag cgcggcagtg agcattctga tgaaatgacg ccacagggtt ttgtgactaa
     5941 ccatgcgggt ggcattttgg gtggcatttc tacggggcag gaaattcgtg tgaatgttgc
     6001 cttaaaacca acttcaagca ttccccaaga acgccgctca atagataaaa atggcaacgc
     6061 cgtgaccatg caaacaacag gccgacatga cccgtgcgtg ggcgtgcgcg caacgccgat
     6121 agctgaagcc atgctcgcct tggtgctgat ggaccatgct cttcgccacc gtgcgcaaaa
     6181 tgcggatgtg accagttcaa cgcctatact taagtaagtt aatcgtgctt gatgtatatc
     6241 atagcaaagc atttgccgtg ggctgataat gaactcaggt cttatgcaac gcgcgtaagg
     6301 taagttgatt tgtcgataag gagattttat gagcattgag caccacgatt tagtccacga
     6361 gttaccagag catcgtgaaa aaatacacag tatgaaaatg agcaatgcac actttgcaaa
     6421 gttatttgac gagtatcatg tggtcaccaa cgaggtggag cagattgaaa aatcaggtgt
     6481 gccggtgtct gatgtggatt ttgaaaatca gaagaaaaag cgggcacagt taaaagatca
     6541 gttatattct atgctgattt cttaatacag tatttttgaa tttaattcta gaaatttaac
     6601 tctaagaatt aaatccgcaa caaaaaagtc ggcttctgcc gacttttttg ttgcggtaag
     6661 cctttggtag cagctatatt ggtgggcggg tttgatcctc gaccttattt gtaaaattta
     6721 ttttgttatt tgatagaaga ctaaattttg agccacgcgc ttcactttaa cctttctcgt
     6781 ttttacttta tttactactt ctttgtgggc gcatttgtgc cctattgggg cttgtattta
     6841 gcagcagaaa aattttcgcc ggctgatatt ggcattttga tgtcgttgtt tcaaatcagc
     6901 cgtatttttg cgcccaattt ttggggttgg ctggcagacc acaccgccaa gcgtgtggtg
     6961 tggattaagc tcacagcggt acttggctta cttggttttg ttgccgtatt ttgggcgcat
     7021 ggcttttggg cgatattttt ggtgatggct gccttgagtt tatttaccag ttccactttg
     7081 ccgctttcag aatccctcac gctggcgcat ttggccacca ccaacggcca ttacagccgt
     7141 attcgcatgt ggggttcact agggtttatc gtcgcgtcgg ttattttggg ttatgtgact
     7201 gaagcctttg gtattcgaag tttgttatgg tttttgttgg cggtgcagat tgttttattt
     7261 gcgcttacgt tcaaattgcc tgaagcaaaa gttgccccgc atgcgcatga tgaattttct
     7321 atctggcaag tggtgcggca gccaaacgtg attgcgttat tgctagggtg cgcgctcatg
     7381 gtaacggcgc acggggtttt atataatttt tattcgattt atttagtgca gcatggctat
     7441 agcaaaagca ccattggctt attgtggtct gttggcgtca tctgtgaaat tggtattttc
     7501 atgtggatgc caaaaatcat ggcgcgctat agtctaaaaa cgatattgct cacaagcctt
     7561 gccttggctg tcctccggtt tagcctcatc ggcgttgcaa ttgatagcct tttcatgatt
     7621 gtgcttgcgc aaaccttgca tgcagcaacg tttggcagct ttcatgcggc ctcggttgaa
     7681 gtggtgacgc agttttttaa tggccgccat caagccaaag gccaagccat atacaacagc
     7741 gtggcgtatg gcgtgggcgg tacagtgggc ggtgtagcag gcggctttgg tttgcaatat
     7801 ctaggacaat tggccaattg gtacggctgg acatattcaa gtgggcagat cacctttatg
     7861 ttggccgcga tttttccgct cattggcttt ttggtgatag gattaggctt aaaattaacg
     7921 ccgcaaaatc aaacacaggg gatgtttaga tgaaggtcat cataaaattt tgcttttgct
     7981 tttgctttat attactgaca gccaacttgg cgcaggcggc aacgcaagtg aatattgtcg
     8041 gtttgttcag caataaagcc gttgttatta tcaatggcgg aaaaccaaaa acgctgagtg
     8101 ttggccaaac cagcgagggc gtgaaattgc tcgcggcaga tagccaaaca gcgactttgc
     8161 aaatagaagg caaaacgcaa caactgggca tggggcaggc cgcctcggta ggtggcaata
     8221 gtgccaatgc cggcgttgcg agtgtgacgc tgtatgctga tacggcaggc cattttattt
     8281 cagaatgtta tgtgaacgga gcgccactga aatttttact cgatacaggt gccacaacgg
     8341 ttgcacttaa cagcggggac gcaaaatttg ccaaaattga ttataagcgc ggcgagccaa
     8401 tccaagtaag cacagccaat ggcgtggtga cagcctaccg cgtgaccatt gctacattaa
     8461 aaataggcgc gctgacgtta aaccaagtag aagccagcgt gctagaaggc ggttcgccct
     8521 cggtggtgtt gcttggcatg agtgccctta atcggctgga catgaaacgt caggatattg
     8581 cactgacgct cagtaaaaaa tactagccca attttatcaa tataaatcgc tgtaatttag
     8641 ggcgttggtg gcgccaattt ttaccgagaa aaaattaatg ccctgagagc ctttatttat
     8701 aaggctcgca gggcaggtga aaagggttgc gcttaaattt agcaggtttt tgctaaatca
     8761 gttcaagtat tttagtcgtc cttcaacaca aaagtcactt tcattactac gcggtactct
     8821 gtcaccttgc catctttcac tttaacactt tggtcttgca cccaagcacc cgtaatgcca
     8881 tgtaaagact cggcagcttt agcaacacca tttgatacgg catcttcaaa acttttttgg
     8941 cttgaggcga tgatttcgat aacttttgca actgacataa tgcgctcctt ttgttgattg
     9001 ttaaaaagtg cctacagatg atgttgagtc gatactttga taccgttcac aattaaagca
     9061 taaagacgac taaaacttag ttaattttat ttacttaacg cgtttgtcac tctcaattaa
     9121 agcataagca ctgtggttgt gaatgctctc aaagttttca gattccacgg tgtaggcgtc
     9181 gatacgtttt tctttgttca gttgggccgc tacatcacgc accatgtctt cgacaaattt
     9241 tgggttgtca taagcgcgct cggtgacaaa tttttcatct ggacgtttga gtaagccgta
     9301 aagctggcat gacgcttggt tttcaaccaa atcaataatg tcttcaatcc aaataaagtc
     9361 gttgataagc gcggtaacag tgacgtgcga acgctggttg tgtgcgccgt aatcagagat
     9421 ttttttgctg catgggcaaa ggctggtcac aggcaccagc actttaacgg taatgtcaaa
     9481 tttgccgtgt tcaatggcgc ctataaaggt gacttcgtaa tcgagcagac tttttacgcc
     9541 agaaatcggt gcggctttat tcacaaaata cggaaaggtc atttccacat ggcctgattc
     9601 ggcttcaagc cgtttaacca tttcgcgcaa aattgtttca aaagactcaa tagaaatcgc
     9661 atgttcattc atgttgagaa tttcaacaaa acgtgacatg tgcgtgcctt taaactgttg
     9721 cggcaaatgc acatacatat taaacatggc aacggtatgc tgctcgccgc ctgagcggtc
     9781 tttaacaatc acggggtggc gaatcgcttt gatgcccact ttatcaatcg caatgtggcg
     9841 ggtatcgacg gtgttttgta cgtcttcaat cgcagcagga tgtgtggatt tagtcatgat
     9901 attccagttc tcgatagggt ctcaggttgc ataatacctg agtataacac taaggtatta
     9961 tttgctcaat ggcggcaatg atgccgcttg catctagacc acacgcagcc aacatggttg
    10021 catggtcgcc atgttcgatg aaagtatcgg gcaagccaag gcataaaatc ggggtgacga
    10081 tgcgcatggc ttgcaatgct tccatcacgg cacttcctgc accgcccatg acggcatttt
    10141 cttcaatggt gacgattaaa tcgtgatttt ttgcgagttc ggcaatcaat gcggtatcaa
    10201 gcggttttac aaaacgcatg ttagccacgg ttgcatctat tttttctgcg gcttcaagtg
    10261 ctggtgtgag catcgagccg aaggctaaaa tagcgacttt ttgcttaggt ttatgactac
    10321 taagcttaat ttcacctttg ccaatggcaa tggcgttaag tgccgcttct gttgcaacgc
    10381 ctgtaccact gccgcgcggg tagcgcactg cggctggccc atgatgttga aagccggtgg
    10441 tgagcatttg cctacattca ttttcatcac tgggcgccat gatgatgata ttgggaatgc
    10501 atcttaaaaa acttaaatca aaactccctg cgtgggtggg gccatctgcc cccacgaggc
    10561 ctgcgcgatc aatggcaaac aatacgggta aattttgtaa ggcaatatca tgaataagtt
    10621 ggtcgtaagc gcgctgtaaa aacgtgctgt aaattgccac cacaggtttt aggccatcac
    10681 aagccatgcc cgcagcaaag gtgagcgcgt gctgctctgc aatgccaaca tcaaaatagc
    10741 gctgcggata tttttcagca aacgcattga gaccagagcc atcacacatc gcaggcgtaa
    10801 tgccaatcag gcgctggtcg gcatctgcca tatccaccag ccattcgcca aacacttgtg
    10861 tgtaggtttt ttttgaaaca ctggtaatcg catcgccgtt aaaggggtca aatttgccaa
    10921 ccccgtgaaa tttattgggg ttgtcttcgg caggctcaaa gcctttacct ttttgcgtga
    10981 cgatatgtaa aaactgcgcg cctttgagct gcttgatatt ttgaaggctt gtgataagcg
    11041 cttctaaatc atgtccgtca atggggccga tatagttaaa gcctaactct tcaaatagtg
    11101 tgccgggcaa taccatgcct ttaacatgct cttctgcgcg cttggcaaat tcttgcatgt
    11161 gcggcatgcc agacaaaact tttttgcccg atttgcgtat cgtgccataa aagcggctag
    11221 cgagtaattt tgctaaataa cgatttaagc cgcctacatt gtctgagatg ctcatgtcgt
    11281 tgtcgttcag tacgaccagc aagtttgcat ccatattgcc tgcgttatta agtgcctcaa
    11341 acgccatgcc gccggtgagt gcaccgtcac caataatcgc aacagcccga cgctctaagc
    11401 ctgctttttg cgcggcaacc gccatgccaa gtgcggcaga aatagaagtg ctggaatgcc
    11461 ctgtgccaaa cgtatcaaat tcactttcgg cgcgacgtgg aaagccggca atgcctttgg
    11521 gtttacgcaa gtgttgcatg gcttcgcgtc tgccggtaag aattttatgc ggatacgttt
    11581 gatggccaac gtcccatacc aagcgatcgt caggggtatt aaatacgtaa tgtagcgcaa
    11641 tggtgagctc aaccacgcct aaattagagg ccaaatgccc acccgttttt gctacgctat
    11701 caataataaa agcgcgtaat tcagcggcta actgcggcaa ttgcgcgcgc tcaagctgcc
    11761 gcagttgtgc gggaaaatca atggcgtcaa ggatgggttg gcgagggaat gaggtcacaa
    11821 gttattttgc cataaatggt tcaaaagagg cgttaagtaa tgttaaaaac ggggtcgtca
    11881 aaagggtcag ttttcttaaa attgtcgctg ggtgataaag ctggcaagtt cgcgtaaacg
    11941 tcttgcctct tcaccaaatg ttaccagcga taaaattgca ttttcataaa gctgtgtagc
    12001 ataggctttg gcttccacta aacccaatag cgtgacataa gtcggctttt ggctcatggc
    12061 atctttaccg gcggttttgc ctaaggtgct ggtgtctgcc tctacatcca aaatatcatc
    12121 caccacttga aaagccaggc caatattgcg cgcatattca cttacagcct tgatttgcgc
    12181 agttgaccca ttaattgcgc ccagcaaaac ggatgcctga ataagtgcgc cggtttttaa
    12241 ctgatgcata ctttcaagct gggtttgcgt caggggtttg ccaacggcgc ctaaatcaat
    12301 cgcctgaccg cccgccatgc cggttgagcc agaggcaatg gctaacatgt gcagcaattg
    12361 cacttgctga tgggcattgg cacataactg cggcgctgct aaaacttcaa acgccaggct
    12421 ttgcaaagca tcgcccacta ataatgcggt ggcgtcatca tactgcttgt ggcaactggg
    12481 tttgccgcgc cgtaaatcgt cgtcatccat gcagggcatg tcgtcatgca ccaatgaata
    12541 cgcgtgaata agctcaactg cgctggcagc cgcatcggca attttcagtt cagtattgca
    12601 caattctgcc gccgcatagc aaagcaacgc acgcacacgc ttgccgccgc cgagcgcact
    12661 gtagcgcatg gccgaatgta atgtttgcgg ggcaacatca gcagcaggca gcgtgcgcgc
    12721 caatacggtt tcaatacgcg cttgctgctg ctgcgcccaa tcttgaaaag cgcctgcgtt
    12781 tggcttttgc atggatgatg cttgcgtatt caaggcggtt acttattctt gattgggttg
    12841 aaaagggtta agctgattgc gcgcatttaa aatttgcact tgctgctcaa cctctgccag
    12901 tgatttttgg caatattgca acagcgcatc accgcgttgg tacgcagcga gggaagcttc
    12961 caaactcatt tggccagatt ccatttgcgc gacgattttt tctaaatccg taagcgcatg
    13021 ctcaaagtta tcgggcgcta cttcttgcgt ggcaagtatg cgggtggatt ttggttttgc
    13081 agagttcatc gcttattttt gttcgcagtt gagctaagtt gctattctac caaatctagc
    13141 gccaattttg gtcgtgcaag cgggcttgaa aagatgaaag aaatagggcg taaacaggca
    13201 ttaaaaggct taaaaatcac accgaccttt ttcgatgctc tggaagcctt ataaataaag
    13261 gctcgtagag cattattttt ttctcgagag attttagctt gcaaaataac gtttttttaa
    13321 acctgaaaaa tggcaaaaat ttatggtaat tttgcaatta aaaatgcacg catgtcatca
    13381 atttcttcta aacacactga atgcgccatc gcatattcat gccaatcaac agggtaatga
    13441 ttgtctagca acacactgcg cgaatttttg cacatgtcca agctgataat gtcatccgca
    13501 acgccatgcg ccataaaaat cgggcagtgg gcattggcaa aattggcttc aactgccaat
    13561 ttgcttttaa gcggtaaata agttgaaagc gccagcacgc ctgccagcgg ttgtgggtag
    13621 cgcagcgcag tgtagagcgc aatggcgccg ccttgcgaaa aacctgccag catgatgcgt
    13681 ttggtagaaa tgccccgtgc cacttcatgc gcaattaggc tgtgaatata gtgctggcta
    13741 acctgtatgc ctgcttcatc ttcaatgggg tagccggttt ggttattggg ttggccgtac
    13801 aggtcatacc aagctggcat gatgaagccg ccattgcgcg tgacgggcat ctctggcgcg
    13861 tgcggtagaa taaaacggat atgcggcaga ttaagcttac tcacaatcgg tgcaaaatcg
    13921 tgcccgtctg cgcctaagcc atgcattaag atgacgctgg cggtgatttg tgcgcctgat
    13981 tggctcagtt ctaaaggggg ttgatggttc atggctgatt caaaatcgtt ggtgaataat
    14041 tcacagtata atgcgcttat gaggcatatc ataatgttag caatggcgga ctaaattacc
    14101 ttcatgagaa tattcacttt aatttcgcag catgcagtgc ggtttgccct tggtgcggcg
    14161 gtgctggtgc tgttgctact gaatgccacg ggttacgtcc atttatcggc tatccaaaag
    14221 ctggagctat ttacctatga cacaaggctg aatatgctgc tgcctaaagg cgtggatgaa
    14281 cgcattgtga ttattgatat tgatgaaaaa agcctgcagg cacaaggccg ctggccgtgg
    14341 gggcgcaata agctcgccga cctagtgaat attttatttg atgattacca tattcaagtg
    14401 ctgggttttg atatggtgtt tgcagaaaaa gatgaaagct cgggccttaa acacttggag
    14461 gctattcaaa gccaatatct atccaccgac cccgagctag ggcaggcatt aggtcaggca
    14521 gttgaaacat taaggcctac gcttgatttt gatgcgttgt ttgccaacag tctaaaaaat
    14581 cgaaatgtgg tgctggggta tgtgttttcg catgatgtgg cgtcggtggg tagtggtgcc
    14641 acatcatgcg aaaacacata ccccagcacc acatttcgat tttttagac
//
LOCUS       DAOQSI010000033        14613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_3193_length_14613_cov_6.161492, whole genome shotgun sequence.
ACCESSION   DAOQSI010000033 DAOQSI010000000
VERSION     DAOQSI010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 14613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14613
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3193_length_14613_cov_6.161492"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            420..2006
                     /locus_tag="PKL42_03640"
     CDS             420..2006
                     /locus_tag="PKL42_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNU66197.1"
                     /translation="MTTETTLNFNELGLTEPILRAINDAGYTTPTPIQAKAIPVVLGG
                     GDLLAGAQTGTGKTAGFTLPILHLLSQKPLATKTTGRPRCLMLTPTRELAAQIEESVG
                     TYGKYLPLTSTVIFGGVNANPQIARLKKSLDILVATPGRLLDHANQKNVDLSGVEILV
                     LDEADRMLDMGFIRDIKKILAMLPKQRQNLLFSATFSDEIKTLADGLLHNPGFVEVAR
                     RNTASELVEQTVHMVAQSHKRELMSYLIQKNDWQQVLIFTRTKHGANRLAEKLIKDGI
                     NAAAIHGNKSQGARTKALAQFKDGTMRVLVATDIAARGLDIDQLPQVVNFELPNVPED
                     YVHRIGRTGRAGSSGAAVSLVDREELKLLKDIEKLIKREIPKVQVEGFTPTDKPEPEA
                     PRSTQPHGRPPRGHGQGQQQRKPREANANGANAGRNQNRNGASRNGQPQVRNQKTYVE
                     ESILIQGHQPKPQGQPTKNLSEAARHQSMPQTGLFVPTVPSRPATSHRPPNSQNTAHG
                     KPRSPNANPNHMHRNGGRGR"
     gene            2124..2603
                     /locus_tag="PKL42_03645"
     CDS             2124..2603
                     /locus_tag="PKL42_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNU66198.1"
                     /translation="MQVTMNTVVTMTYELKDTDGNVLESSTEPVAYLHGGYDNIFPKV
                     EEAIHGKNVGDVVIVDLQPDDAFGEYDEELVQIEPASAFPAADLKVGMQFEGEDETGE
                     VILYTVTEIADGKVVVDGNHPWAGQRVVFTATITDVRAANQEEVSHQHVHGAGGHHH"
     assembly_gap    2675..2774
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2775..5177)
                     /gene="mutS"
                     /locus_tag="PKL42_03650"
     CDS             complement(<2775..5177)
                     /gene="mutS"
                     /locus_tag="PKL42_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNU66199.1"
                     /translation="MSSFENHTPMMRQYLALKAQHPDMLLFYRMGDFYELFFEDAEKA
                     SRLLGITLTQRGSSNGAPIKMAGVPYHAAEGYLAKLAKLGEAVVICEQVGDPATSKGP
                     VERQVARILTPGTLTDTALLDESRDNQLLSIFYADNLVGLARLNLASGTFILSEIALG
                     LLGQEIERIAPSEILLPDDFQHAAIRSSKVAKKRLSPWQFDYDSAFNTLTKQLNTRDL
                     NGFGCAELKPAICAAGAMLDYVQHTQRTTLPHINAVSVESTSAYVQLDAATRRNLEID
                     ITLRGEAAPTLYSLLNTTQTAMGARLLRHWLHHPLQDRNLVLKRHEAIAELIDSNGFQ
                     TLARTLKAVGDIERITARVALKTARPRDLSSLAASLQQLPLLQSDLAIMQTSLLQTLG
                     ANLTAPSAVVTLLTTAIQPEPSTVLREGGVIADGYDAELDELRSLQNNHGEFLLQYEA
                     AEKARTGIANLKVEYNSVHGFYIEMSRTQAESAPAEYRRRQTLKNVERFITPELKAFE
                     DKVLSANDRALAREKLLYAQVLEQLMPFITPLQTNAGAVASLDVLCSFAERAVALKYV
                     APQFSTEAGIQIVGGRHPVVESIAQPFVANDVILNPYRQLLLITGPNMGGKSTFMRQT
                     ALIVLLAYCGSYVPATSAKIGEIDRIMTRIGASDDLAGGRSTFMVEMTETANILHNAT
                     DKSLVLLDEIGRGTSTFDGLSLAWAVAKQLLEKNHSYTLFATHYFELTRIVDEFKYAA
                     NVHLDAIEHGHNIVFMHKVEEGAANQSYGLQVAQLAGIPKSVVASAKRKLQQLEASQI
                     AQH"
     gene            complement(5170..7176)
                     /locus_tag="PKL42_03655"
     CDS             complement(5170..7176)
                     /locus_tag="PKL42_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HNU66200.1"
                     /translation="MIEQAFAQHEQQPDAPKLIATLVAELRPTKSHQTEVAVKAIQAL
                     CFLLNSDANKAKLLREAILQLLAEHKPISLYLIARHSVFTGFFSEMRRRISHKFLPEA
                     INEGYLIDLFARFFTKNTDAIWVSAVPNEVWQQLIVAIGFQDAPSHLKAESKQNLLAA
                     TQVLSYRIAALGLEPELLRNHPELEQYTSPFLAQHAVLSEFLISINTLSGQPDQEAWP
                     SHDIDHILVMLSQSSAVISKIHRNSAQVGTSIQLTHITQQMTKQIARLELLLRIFASF
                     DKPDSAHTEVVSLFKQLVYSECHKNDVSEHWHENMEVMALRVTENASRTGEHYITDSR
                     PEYFGLMRSAMGAGVIIALMAMLKILLSKMQLAPLTEAILFSLNYGLGFILIHILHFT
                     VATKQPAMTAAAIAASIDAADSQSKAVDNLVAMIANTTRSQMVAIFGNVVLAIPVAML
                     LAWITLLATGTHFITPDKAHHLLESIDPFNSGALIYAGIAGVCLFLSGLIAGYHDNMA
                     IYNKIPQRLRALSWLQLLLGKSLLARVANYIENNLGALAGNFYFGCLLGGMTAIGVLF
                     GLPFDIRHIAFSSAFVGFAAVGLEFMLTWQAALYAALGLVLIGLVNLSVSFSLALYVA
                     MKSRGVRFKQWRLLIARLATRLNQHPAEFVLPPKKLEKQSGTHE"
     gene            complement(7228..8034)
                     /locus_tag="PKL42_03660"
     CDS             complement(7228..8034)
                     /locus_tag="PKL42_03660"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNU66201.1"
                     /translation="MHPMLTIAVKAAREAGKIINRASQDVGAIKIQTKTYNDFVSDVD
                     RAAEQAIISVLKDAYPDHGFLGEESGESNQQADNIWIIDPLDGTTNFLHSFPQYCISI
                     ALQQKGVLTQAVIYDPVRNDLFTATKGRGAFLNDKRIRVTNRSKLQDSLIATGFPFRD
                     FTHLETYLNMLRDMIKKTTGIRRPGSAALDLAYVAVGWVDGFFEINLSAWDIAAGGLI
                     VQEAGGIVGDFEGNESWLKTGNIVAANPKVFAQILQTLSPHLTDDLKTPK"
     gene            8097..8852
                     /locus_tag="PKL42_03665"
     CDS             8097..8852
                     /locus_tag="PKL42_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNU66202.1"
                     /translation="MSQLIFNNIPVFNNIRVVLCQTSHPGNIGSAARAMKTMGLQHLY
                     LVKPQHFPDAHATALSTGAADVLENAIVTETLSEALTGCVLAIGMSARKRQISHELVN
                     VREAALRASKIASTQPIALVFGTEMSGLSNAELDCCQLLAMIPANPEYSSLNLAAAVQ
                     VMCYEVRMAVLEGKLEATPSPELATNEALEGFYAHLEETLIQIGYLNPSAPKKLSERI
                     RRIYARAQLEREEVNLLRGILTLSLTPKKHTKY"
     gene            8949..9989
                     /locus_tag="PKL42_03670"
     CDS             8949..9989
                     /locus_tag="PKL42_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNU66203.1"
                     /translation="MRKTLINFALLLFAMPAFALPEDGLVYQLKASVVKIRVILPNGH
                     YGVGSGVVVAKDQVVTNCHVVANAAAINVIKFGESFRASSIKADWQHDICIVRFDGLN
                     TEVAQIGSSKNLQYEQPVFTISFPNNAPKPLATYGFVKALYPMDSSVIIRSTSDFRMG
                     ASGGAMFSDDGKLVGIITLKSPGRNAFYYNMPVEWIAQVQNQPEQAIPAIGAPPFWDA
                     LEENRPYFMRVVQPYQTEDWPALLSLATRWTEQEPAAIEAWYYRAVAEYKLKNIAEAI
                     KHFNQVLAQNSRHTNALLYLALMDAENGNKEAALAKAKQLDALDVEAANQVKVALGLP
                     AKTEAKDCQATC"
     gene            complement(10158..10577)
                     /locus_tag="PKL42_03675"
     CDS             complement(10158..10577)
                     /locus_tag="PKL42_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNU66204.1"
                     /translation="MLRTKIKRRVTLMLALGMLAGMPTTLLAKEKLKHPSSAVSKQKP
                     MVGLASFYAAKFHGRRTASGEIFITHALTAAHLTLPFGTKVKVTNLRNGKSVIVRIND
                     RGPHVRGRIIDLSVAAAKKIGLNRAGTVRVKLEILGK"
     gene            10688..>11326
                     /gene="cysE"
                     /locus_tag="PKL42_03680"
     CDS             10688..>11326
                     /gene="cysE"
                     /locus_tag="PKL42_03680"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNU66205.1"
                     /translation="MFNHIKEDISIVFERDPAARTRFEILTTYPGVHALIMHRFSHWL
                     WKADFFWLGRFFSHIGRWLTGIEIHPGATIGRRVFIDHGMGVVIGETAVIGDDCTLYH
                     GVTLGGTSWNKGKRHPTLEQGVVIGAGAKVLGPITIGKNAKIGSNAVVVKDVPENATA
                     VGIPARILEEEKSKQREATAQKIGFAAYAVGNDENDPMIKALHSLLDHANKQD"
     assembly_gap    11327..11426
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11531..12043
                     /locus_tag="PKL42_03685"
     CDS             11531..12043
                     /locus_tag="PKL42_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcription factor"
                     /protein_id="HNU66206.1"
                     /translation="MRLTTKGRFAVTAMLDLALHEAEELAAQVTPIQQGVGSKPVTLA
                     GISERQHISLSYLEQLFSRLRKDGLVSSVRGPGGGYRLAKPASEISVSNIITAVDELI
                     DATQCGGTENCHDEGRCMTHDLWTALNGKILEYLSGVSLAELVANQLNKQNNQPVQFT
                     LKRENAQLRA"
     gene            12063..13208
                     /locus_tag="PKL42_03690"
     CDS             12063..13208
                     /locus_tag="PKL42_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HNU66207.1"
                     /translation="MSHVYFDHNATTALDSRVLKAMLPFLTTQQGNATSRHAYGRFAR
                     DAVEQAREQVANAVGAYPSQVVFTASGTEANNFAIAGMAATLPNTQIAISAIEHPCVT
                     RPAEMMQSRGWTLAKIGVNHQCELAFAQLENALRKPTSLVSVMLANNETGTIQNIADV
                     AELARKNGAYVHTDAVQALGKIDVNFEALNVHAMTISSHKIHGPQGAGALILDKRVDI
                     SPLLLGGGQEKGLRSGTENVAAIVGFGMACALAVEKLHEHALHTQSLRQQLEIDLQAL
                     DATIFSHHTARLPNTSFFAFPNIEGETLVMALDKKGFAVASGSACSSDSTEPSHVLLA
                     MNLPPDLARGAIRVSFDASNTVEQVSAFLTILKQELKQLQQMVAIAA"
     gene            13248..14462
                     /locus_tag="PKL42_03695"
     CDS             13248..14462
                     /locus_tag="PKL42_03695"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HNU66208.1"
                     /translation="MDRDPIYLDYSATTPVDPRVAAKMIPYLTEHYGNPASRSHPYGW
                     TAEKAVENAREEVAKLVNADPREIVWTSGATESNNLAIKGAANFYASTKGKHIITVAT
                     EHKAVIDTVRELERQGFTATYLEPEPTGLVDLEKFKAAIRPDTVLASVMLVNNEIGVI
                     QDIEAIGKICREHGVIFHVDAAQATGKVAIDLEKLPVDLMSFSAHKTYGPKGIGALYV
                     RRKPRIRIEAQMHGGGHERGMRSGTLATHQIVGMGEAFRIAREEMEMENARIRKLRDR
                     LLTGLQSIEETYVNGELDHRIPHNLNISFNYVEGESLIMAVKGIAVSSGSACTSASLE
                     PSYVLRALGRSDELAHSSIRFSIGRFTTEADVDYTIALLKEKISKLRELSPLWEMYKD
                     GIDISKVQWAAH"
BASE COUNT         3987 a         3446 c         3353 g         3627 t
ORIGIN      
        1 caaaagcgcc ttattttaag cacaaccttt ttcgatgccc tgcgagcctt ataaacaaag
       61 gctcgcaggg cataaaaatt ttctcgggcg attgaatgcc aacattcaag ataaaattaa
      121 tatgcatttt tacttgaatt tttatcactt caccccattt acaaattagc ttggctgttt
      181 ttaaaaatat cgcctaaaaa atatagcttt aaaaatacaa agctcgcttt aatcgtaatc
      241 ttccgtataa tgccaagcac gtaactcaaa agccctatgc caaccaatca gcgtagcggt
      301 ctctttactt aaaatctttc tgatttttca aatcacgcta aagataaaac tttgccttcc
      361 tcgattggtg ttgcttcaaa gtgataggtc ggcttaattc ctagctggag ctatatacat
      421 tgacaactga aacaacgctt aactttaacg agttaggtct gactgaaccc attttacgtg
      481 cgattaacga cgcaggttac actaccccaa ccccaattca agccaaggcc attcccgtag
      541 ttttaggcgg tggcgattta ctcgccggcg cacaaacggg cacaggcaaa acggctggct
      601 ttacgctgcc gattttgcat ttacttagcc aaaaaccgct ggccaccaaa acaactggac
      661 gaccacgctg tttaatgctg acgcctacgc gagagcttgc agcgcaaatt gaagagtctg
      721 ttggcactta tggcaaatat ttgccgctga cttcaaccgt gatttttggc ggcgtgaatg
      781 ctaacccgca aattgcgcgt ttaaaaaaat cgctcgatat tcttgttgcc acgcctggcc
      841 gtttgcttga ccacgccaat caaaaaaatg tcgatttatc aggcgttgaa attttagtgc
      901 tggatgaagc cgaccgtatg ctcgatatgg gctttattcg cgacatcaaa aaaatattgg
      961 ccatgctgcc aaagcaacgc caaaatttgc tgttttcagc cacgttttcg gacgaaatta
     1021 aaacgctggc cgatggcttg ctacacaacc ccggctttgt tgaagtggcg cgccgcaata
     1081 cggcaagtga attggttgag caaaccgtgc acatggtggc acaaagccat aaacgtgagc
     1141 tgatgagtta tctcattcaa aaaaatgact ggcagcaagt gttgattttt acccgcacga
     1201 aacacggcgc caaccgtttg gcagaaaaac tgattaaaga tggcattaac gcagcggcga
     1261 ttcatggcaa taaaagccaa ggcgcgcgca ccaaagcgct ggcgcagttt aaagatggca
     1321 cgatgcgcgt attggtggcc acagatattg ctgcacgcgg cttggatatt gaccagttgc
     1381 cgcaagtggt gaattttgaa ttgcccaacg tgcctgaaga ctacgtgcat cgcatcggtc
     1441 gtaccggccg tgcaggcagt agcggtgctg cggtatcatt agtagatcgt gaagaactca
     1501 aactgctgaa agacattgaa aagctgatta aacgcgagat tccaaaagtg caggtggaag
     1561 gctttacgcc aacagacaaa cctgagccag aggcgccacg ctcaacccag ccgcatggca
     1621 gacctccgcg cgggcatggt caaggccagc aacaacgtaa gcccagagaa gccaacgcca
     1681 atggcgccaa tgcaggtcgt aatcaaaaca gaaatggcgc atcgcgtaac ggccaaccgc
     1741 aggttagaaa ccaaaaaacc tacgttgaag aaagcatttt aattcaaggc catcaaccaa
     1801 aaccgcaggg tcagccaact aaaaacctgt ctgaagcggc acgccatcaa tccatgccgc
     1861 aaactggttt gtttgtgcca accgtgccca gtcgtccagc aaccagccat cgcccgccaa
     1921 atagccaaaa tacagcgcat ggcaagccac gtagcccaaa tgccaacccc aatcacatgc
     1981 acagaaatgg tgggcgtggc cgttaggtgt taacttttaa taaattaacg cgcagaatat
     2041 ttttgggaat atcgaaaata gattaaaatt gcaccacctg ttttgcgcac aaccccttac
     2101 caataatgat ctaggaactc atcatgcaag taaccatgaa taccgtagta acaatgactt
     2161 acgaacttaa agataccgac ggcaacgtgc tggaatcaag taccgagcca gtggcttatt
     2221 tgcacggcgg ctacgataat atttttccaa aagttgaaga agccattcac ggcaaaaatg
     2281 tgggggatgt agtcattgta gatttacaac ctgatgatgc ctttggcgag tatgacgaag
     2341 aactggtgca aattgagcca gccagcgctt ttccagcagc tgatttaaaa gtcggcatgc
     2401 aatttgaagg cgaagatgaa acaggcgaag tgattttata caccgtgact gaaattgccg
     2461 atggcaaagt ggtggtggat ggtaatcatc cttgggccgg ccaacgcgtg gtttttactg
     2521 caacaattac tgatgtgcgt gcagccaacc aagaagaagt ttcacaccag cacgtgcatg
     2581 gcgcgggcgg gcatcaccac taatttttag ccgtcgctca tagtagaaaa gcacgcatat
     2641 tttgcgtgct tttttttggt tttttaatct aaaannnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnatgttg cgcaatttgg ctggcttcta actgctgtaa cttacgcttg
     2821 gcactcgcca cgactgactt tggaataccc gctaactgcg ccacttgtaa gccgtaactt
     2881 tgattggcgg caccttcttc cactttgtgc ataaagacaa tattgtgtcc gtgttcaatg
     2941 gcatctaaat gcacattggc cgcgtatttg aattcatcga caatgcgcgt gagctcaaaa
     3001 taatgcgtgg caaagagtgt ataactgtgg tttttttcta atagctgttt ggccaccgcc
     3061 cacgctaaac tcaggccgtc aaaggtgctg gtgccacggc caatttcatc gagcagcact
     3121 aaacttttgt ctgtcgcatt atgcaaaata ttggcagtct cggtcatttc caccataaag
     3181 gttgacctgc cccccgccaa atcatccgat gcgccaatac gcgtcataat acggtcaatt
     3241 tcaccaattt tcgccgaagt tgctggtaca taactgccac aataagccag cagtacaatc
     3301 aaagcagttt gtcgcataaa ggtcgattta ccgcccatat tggggcctgt aatgagcagc
     3361 aactggcggt atgggtttaa aatcacgtca tttgccacaa acggttgagc aatgctttca
     3421 accacaggat ggcgaccacc aacaatttgg atgcctgctt ccgtgctaaa ttgcggcgca
     3481 acatatttca gcgccaccgc gcgctcggca aagctgcaca gcacatccaa gctagctacc
     3541 gcgcctgcgt tagtttgcaa gggggtgata aatggcatga gttgttcaag cacctgtgcg
     3601 tagagcaatt tttcacgcgc cagtgcgcgg tcattggcgc taagcacttt atcttcaaag
     3661 gctttgagtt cgggcgtaat aaagcgctca acatttttaa gcgtttggcg gcggcgatat
     3721 tctgcaggcg cgctttctgc ctgtgtgcgg ctcatttcaa tgtaaaagcc atgcacgctg
     3781 ttgtattcca cttttaagtt ggcaataccc gtgcgtgctt tttcagcagc ttcgtattgc
     3841 agtagaaatt ctccgtgatt attttgcaag ctgcgcaact catccagctc ggcatcatag
     3901 ccatccgcaa tcacaccgcc ctcacgcaat acagtactgg gctctggctg gatggcagta
     3961 gtcaataacg tcaccaccgc actgggcgca gttaaatttg cacctaaggt ttgcagcaaa
     4021 cttgtttgca tgatggccaa atccgattgc aacaagggta attgctgcaa gctggccgca
     4081 agacttgata aatcgcgtgg tcgtgccgtt tttaacgcca cacgcgcggt aatgcgctca
     4141 atatcgccca ccgctttcag cgtacgcgcc agtgtttgaa aaccgttgct atcaattaat
     4201 tcggcaatgg cctcatggcg tttaagaacc agattacggt cttgcaatgg atgatgcaac
     4261 caatggcgta acaaacgcgc gcccatggca gtttgcgtgg tgttaagtag tgaatataac
     4321 gttggcgcgg cttcgcctcg cagtgtgata tcaatttcta agtttctgcg cgtggcggca
     4381 tccagttgca cataggcact ggtggattca acactcactg cgttaatatg cggcaaagtg
     4441 gtgcgctgtg tgtgctgaac gtaatccagc atggcccctg cggcacatat ggcgggcttt
     4501 aactcagcac aaccaaaacc gttcaggtcg cgcgtattta actgtttagt aagtgtatta
     4561 aaagcgctgt cgtaatcaaa ctgccaagga cttaagcgtt ttttagccac tttgcttgaa
     4621 cgaatggccg catgctgaaa atcatctggc aacaaaattt cactcggtgc aatgcgctca
     4681 atttcttggc caagcagccc caatgcaatc tcacttaata taaacgtgcc agaagctaaa
     4741 ttgagccgcg ctaaaccaac aaggttatct gcataaaaaa tacttagcaa ctggttatcg
     4801 cgcgactcat ctaaaagcgc agtgtcagta agcgtccctg gcgttaagat gcgtgccact
     4861 tgccgctcaa ccgggccttt gctggtggcg gggtcaccca cttgctcgca aatcaccacg
     4921 gcttcaccca atttcgccag cttggctaaa taaccctccg ccgcatggta aggcacgccc
     4981 gccattttga tcggcgcgcc gttgctggag ccgcgctggg ttaaggtaat ccccagcaga
     5041 cgtgaagctt tttccgcatc ttcaaaaaat aactcgtaaa aatcccccat gcgataaaac
     5101 aacagcatgt caggatgctg cgccttgagc gcaaggtatt ggcgcatcat cggggtgtgg
     5161 ttttcaaaac tactcatgcg taccggattg cttctctagc ttttttggtg gcaatacaaa
     5221 ttctgctggg tgctgattta aacgtgtggc taaacgagca atcagcaagc gccactgttt
     5281 aaaacgcaca ccgcgcgact tcattgcgac ataaagcgct aggctaaagc tcacgcttaa
     5341 attcacaaga cctattaaaa ctaagccgag tgcagcatag agagctgcct gccaagtgag
     5401 cataaattct aggccaactg cggcaaaacc cacaaatgcg gatgaaaacg caatgtggcg
     5461 aatatcaaac ggcaagccaa acagcacgcc tatggccgtc atgccaccaa gcaagcagcc
     5521 gaagtaaaaa ttccctgcaa gcgcccctaa attattttca atataatttg caactcgcgc
     5581 cagtaacgat ttgcctaaca atagttgcag ccaagatagg gcgcgcagtc gttgcggtat
     5641 cttattgtat atcgccatat tatcgtgata gcccgcaatc aagccagata aaaatagaca
     5701 tacgccggca atgccggcat aaatcaaggc gccactatta aaggggtcaa tactttccaa
     5761 taagtgatgc gctttatcgg gtgttataaa atgcgtacct gtggccaaca aggtaatcca
     5821 tgcgaggagc attgccacag gaatcgctaa caccacgtta ccaaaaatcg ccaccatttg
     5881 actacgtgtt gtgttggcaa tcatcgcaac caagttatcg accgcttttg attggctatc
     5941 ggccgcatca atactggcgg caatggccgc agctgtcatg gcgggctgtt tggtggccac
     6001 ggtaaaatgt agaatatgaa tcaatataaa acccaaacca taatttaagc taaaaagaat
     6061 ggcttccgtc agtggcgcca attgcatttt actcaacagt attttcagca tggccatcag
     6121 cgcaatgatc acacccgcac ccatggcgga gcgcatcagg ccaaaatatt caggccgact
     6181 atctgtaata tagtgctcac ctgttctgct ggcgttttct gttacacgta gcgccattac
     6241 ttccatattt tcgtgccagt gctcagaaac atcattctta tggcactcac tataaacgag
     6301 ctgtttaaac aaactgacaa cttctgtatg ggctgaatct ggtttatcaa aacttgcaaa
     6361 aatcctcagc aatagctcta accgggcgat ttgcttggtc atttgttgtg tgatgtgtgt
     6421 cagctgaatg cttgtaccca cttgggcgct attgcggtga atcttactga ttaccgcact
     6481 agattgacta agcatcacta gaatatggtc aatatcatgg cttggccatg cctcttgatc
     6541 aggctgaccg gacagcgtat taattgagat taaaaactca gaaagcactg catgctgtgc
     6601 cagaaatggt gaagtgtatt gctctaactc aggatgattg cgcaacaact ctggctctaa
     6661 ccccaaagcg gcgatccgat acgaaagcac ctgcgtagca gccaataaat tttgcttact
     6721 ctcggccttt aagtggctgg gggcgtcttg aaaaccaatc gccacaatca gttgttgcca
     6781 cacctcatta ggtacggctg acacccaaat ggcatcggta tttttagtaa aaaaacgtgc
     6841 gaataaatca attaaatacc cttcgttaat agcctcgggt aaaaatttgt gtgaaatgcg
     6901 tctgcgcatt tctgagaaaa acccagtaaa aaccgaatga cgtgcaatta aataaagtga
     6961 aatcggttta tgttctgcca gcaactgcaa aattgcctcg cgtagcaatt ttgctttgtt
     7021 tgcatcgcta ttgagtaaaa agcaaagcgc ctgaatggcc ttgactgcta cttcggtttg
     7081 gtgcgatttg gtgggcctta attcggccac aagcgttgcg atgagcttag gtgcatctgg
     7141 ctgttgttca tgctgggcaa acgcttgttc tatcatcgct tcacattcta attaagtgat
     7201 tttttcagca gtggctatta ccgccactta ttttggcgtt tttaaatcat cagttaaatg
     7261 cgggctgagc gtttgcaaaa tttgcgcaaa cactttgggg ttggctgcga caatattacc
     7321 cgttttcagc caactctcgt tgccctcaaa gtcgccgaca ataccgcccg cctcttgcac
     7381 gattaaaccg ccagcagcaa tgtcccacgc agataaatta atttcaaaaa aaccatccac
     7441 ccaacctacc gccacatacg ctaaatctag cgccgcagaa cctgggcgac ggatgcctgt
     7501 ggttttttta atcatgtcac gcaacatatt gaggtaagtt tctaagtgcg taaaatcgcg
     7561 gaagggaaag ccggtggcaa ttaacgagtc ttgcagcttg cttctgttgg tgacgcgaat
     7621 acgtttatca tttaaaaaag caccgcggcc tttggtggcg gtaaataaat cattacgcac
     7681 tgggtcgtaa atcaccgctt gcgtcagcac gcctttttgt tgcaaggcaa tcgaaatgca
     7741 atattgcgga aagctatgta aaaaattggt cgtgccatcg agcgggtcaa taatccaaat
     7801 gttatcggct tgctggttgc tctcaccgct ttcttcacct aaaaagccat ggtctgggta
     7861 agcgtctttg agtacagaaa taatggcctg ctcggcggcg cggtccacat cactgacaaa
     7921 atcattatag gttttggttt gaattttaat cgcaccgaca tcttgcgaag cacggttaat
     7981 aattttaccg gcttcgcgcg cggctttgac tgcaatggtg agcattggat gcatggcgta
     8041 gttttcttaa agagctgtta aaatggcatt ttagcattat taggcgcttc cccaaattgt
     8101 cgcaactcat ttttaataac attcctgtat ttaacaatat tagggttgtg ctttgccaaa
     8161 ccagccatcc gggcaacatc ggctcagccg cgcgcgccat gaaaaccatg gggttgcagc
     8221 acctgtattt ggttaaacca caacattttc ctgatgctca cgcgaccgcg ctttccacag
     8281 gggcggcaga tgttttagaa aatgccattg tcaccgaaac attaagtgaa gcgctcaccg
     8341 gttgcgtgtt ggctattggc atgagtgcgc gaaaacggca aatttcgcat gagttagtga
     8401 atgtacgtga agccgcgctt cgggccagca aaatcgcctc aacccaaccc attgccttgg
     8461 tgtttggcac tgaaatgagc gggctttcaa atgctgaatt ggattgctgc caactgcttg
     8521 cgatgattcc tgctaaccca gaatactctt cactcaacct tgccgccgcc gtacaagtga
     8581 tgtgctatga agtccgcatg gcagtgttag aaggcaaact ggaggcaacg cctagccctg
     8641 aacttgccac caatgaagcg cttgaaggtt tttatgcgca tttggaagaa acgctcattc
     8701 aaattggcta ccttaacccc agcgcaccga aaaaactcag cgagcgcatc cgccgcattt
     8761 acgcgcgcgc tcagcttgaa agagaagaag tgaacttatt aagaggcatt ttgacgttat
     8821 cgctcacccc aaaaaagcat actaaatatt agttacacgc tatttgcacg caatgacttg
     8881 aattgggaac ttaaaagctt aaactttctc ttaacttgtt aaatcttcaa acgcttaggt
     8941 gcctagccat gagaaaaacg ctcattaact ttgctttgtt attgtttgcc atgccggcat
     9001 ttgccttacc tgaagatggc ttggtttacc aattaaaagc atctgtcgtc aaaattcgtg
     9061 tgattctgcc aaacggccat tacggagttg gttctggcgt ggtggtggcc aaagaccaag
     9121 tcgtcaccaa ttgtcatgtg gtagccaatg cagctgccat caatgtgatt aaatttggcg
     9181 agagttttcg cgcttccagc atcaaagcag attggcagca cgatatttgc attgtgcgct
     9241 ttgatgggct gaatactgaa gttgcgcaaa tcggctcaag taaaaatctg caatatgagc
     9301 aacctgtttt taccatcagc tttcccaata atgcgcccaa accgctggcc acttacggct
     9361 ttgttaaagc gctctacccc atggatagca gcgtaatcat tcgctcaacc agcgatttta
     9421 gaatgggcgc cagtggcggc gcgatgttta gcgacgatgg caaactggtg gggatcatca
     9481 cactaaaaag cccggggcga aacgcgtttt attacaacat gcctgtggaa tggatagcgc
     9541 aagtgcaaaa tcagcctgaa caagcgattc cggcgattgg cgcgccgcct ttttgggatg
     9601 ctttggaaga aaatcgcccg tattttatgc gcgtggtgca accctaccaa acggaagact
     9661 ggccagcgtt gctttcattg gctacgcgct ggaccgagca agaaccagca gctatcgagg
     9721 cttggtatta ccgtgcggtg gctgaataca aattaaagaa tatagcggaa gcaatcaagc
     9781 attttaatca agtgctcgcg caaaatagcc gccacaccaa tgccttgctg tatttagcct
     9841 taatggatgc cgaaaatggc aataaagaag cggcccttgc aaaggcaaag cagctcgacg
     9901 cacttgatgt tgaagccgcc aatcaagtaa aagtggcgct agggttgccc gctaaaactg
     9961 aagcaaaaga ttgccaagcc acctgttaaa tgttgcgcat tttatacttc taaattaacg
    10021 cttaaaacgc aaccaaatcc tacgagaaaa aataaatgct ctggaagcct tgtaaataaa
    10081 ggcttccaga gcatcgaaaa aggttgcgct taaaacaaca tgctttttaa gtgattttta
    10141 ctttatctaa caccgtttta tttgccaagt atttctaatt ttacccgcac cgtacccgca
    10201 cgatttaatc cgattttttt ggccgccgcg accgataaat caataatccg tccgcgtaca
    10261 tgcgggccgc gatcattgat gcgcacgatg accgatttac cattacgcaa gttggtgact
    10321 ttcactttgg tgccaaaagg caaggtaagg tgcgctgcgg tgagggcgtg cgtgataaaa
    10381 atctcgccac tggcggtgcg tctgccatga aatttggcag cataaaacga agccaaaccc
    10441 accatcggtt tttgtttact gacagcgctt gagggatgtt ttagtttttc tttagccagc
    10501 aatgtggtcg gcatgcctgc cagcatgccc agcgccaaca ttagcgtgac gcgtcgctta
    10561 atttttgtgc gtaacatgca attttccccc gatacaagtg aaggcaatct aaaccaatac
    10621 cacactacca aaaaacttca agatgcacac ctctgccttc aagtatggat tgtggcataa
    10681 taccgccatg tttaatcaca tcaaagaaga catctccatc gtattcgagc gcgaccctgc
    10741 agcacgcacg cgatttgaga ttctcaccac ttatcctggc gttcatgcgc tgattatgca
    10801 ccgctttagc cattggcttt ggaaagccga ttttttttgg ctaggccgat ttttctcgca
    10861 cattggccgc tggcttaccg gcattgaaat tcacccaggc gccaccattg gccgccgcgt
    10921 atttattgac cacggcatgg gcgtggtgat tggtgaaacc gcggtgattg gcgatgactg
    10981 cacactctac cacggcgtta cccttggcgg cacttcgtgg aacaaaggca aacgccaccc
    11041 aacgctcgaa caaggcgtgg tgattggcgc gggcgcaaaa gtgctgggac ccatcaccat
    11101 aggcaaaaac gctaaaatcg gctcaaacgc ggtagtggta aaagatgtgc ctgaaaacgc
    11161 cactgcggtc ggtatccccg cgcgcatttt agaagaagaa aaatcgaaac agcgtgaggc
    11221 cactgcgcaa aaaattggct ttgccgccta tgcggtgggt aacgatgaaa atgaccccat
    11281 gattaaagcg ctacattctt tgcttgatca tgccaataag caggatnnnn nnnnnnnnnn
    11341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11401 nnnnnnnnnn nnnnnnnnnn nnnnnntttt aaggccattc aagcccatga aacttttatc
    11461 cataataatt gactaaatca ctaggttatt atagaattta accctaggaa attaaaaaaa
    11521 gccatttatt atgagactca ccacaaaagg ccgttttgca gtgactgcga tgttagacct
    11581 cgccctacat gaggcagaag aacttgcggc acaagtcaca cctatccaac aaggtgttgg
    11641 cagcaagcct gtaacgcttg cgggcattag cgagcgccag catatttcac tttcatattt
    11701 agagcagctt tttagccgct tgcgtaaaga tgggttggtc agcagcgtgc gcggccctgg
    11761 cggcggttac cgtttggcca aacccgccag tgagattagc gtatctaata tcattactgc
    11821 ggtggatgaa ctgattgatg caacgcaatg cggtggcacc gaaaactgtc atgatgaagg
    11881 tcgttgcatg acgcatgatt tatggacggc gctaaacggc aaaattttgg aatatctgtc
    11941 tggtgtaagt ttggctgaac tggttgcaaa ccagttgaac aagcaaaaca accagcctgt
    12001 gcaattcacg cttaagcgcg aaaacgcgca attgcgggct taaggtaaac acaaaaccaa
    12061 tgatgtcaca tgtgtatttt gatcataacg ctaccaccgc gcttgatagc cgtgtgctga
    12121 aggcgatgtt gccgttttta acaacgcaac aaggcaatgc caccagccgc catgcctatg
    12181 gtcggtttgc acgtgatgcg gtggaacaag cccgtgaaca agtggcaaac gcggtgggcg
    12241 cttatcctag ccaagtggta tttacggcta gcggtactga agccaacaat ttcgcgattg
    12301 caggcatggc cgcaacgctg ccgaatactc aaattgccat cagcgccatt gagcatccat
    12361 gcgtaacgcg cccagctgaa atgatgcaat cacgcggttg gactttggca aaaattggcg
    12421 tgaatcatca atgtgaatta gcgtttgcgc aacttgaaaa tgcacttcgc aaaccaacca
    12481 gtttggtttc agtaatgctt gccaataatg aaacaggcac aatacaaaat attgctgacg
    12541 tggctgagtt agccagaaaa aatggtgctt atgtgcatac cgacgcagtt caggcattgg
    12601 gtaaaattga tgtaaatttt gaagcgttaa atgtgcatgc tatgaccatt tcaagccata
    12661 aaattcatgg tccgcaaggc gctggtgcgc tgattttaga taaacgtgtt gatatttcac
    12721 ccttgttatt aggcggcggc caagaaaaag gcttacgtag cggcacagaa aatgttgcag
    12781 cgattgttgg ttttggtatg gcttgcgcgt tggcagtaga aaaactacat gaacacgcgt
    12841 tgcatacaca gagtttgcgc cagcaattgg aaattgactt acaggcactt gatgccacaa
    12901 tttttagtca ccatacagcc agattgccta ataccagctt ttttgctttt cctaacattg
    12961 aaggtgaaac attagtgatg gcgctggata aaaaaggctt tgcggttgcc agcggttcgg
    13021 cttgttcaag cgacagcacc gagccaagcc atgtgttgct tgccatgaat ttgccacccg
    13081 acctagcgcg gggtgcgatt cgcgtgagtt ttgatgccag caatacggtt gagcaagttt
    13141 cagctttctt aaccatactc aaacaagagt taaagcaatt acaacaaatg gttgcaatcg
    13201 cggcttagga aaaatattta gaaatcaaga aagtaaagtg taaacacatg gatagagacc
    13261 caatttattt agattactcc gccactacgc cagttgatcc gcgtgtagcg gccaaaatga
    13321 ttccgtattt gacggagcat tacggcaacc cggcgtcacg tagccacccg tatggctgga
    13381 cggctgaaaa agccgtggaa aacgcccgag aagaagtggc taaattagtc aacgccgacc
    13441 cacgcgaaat cgtctggaca tccggcgcaa cggaatcaaa caacttggcg attaaaggcg
    13501 cggctaactt ttatgccagc acaaaaggca agcatattat taccgtagcc accgagcata
    13561 aagctgtgat tgacaccgtg cgtgaattgg agcgccaagg ctttaccgcc acttatttag
    13621 aacctgaacc aaccggattg gtggatttgg aaaaattcaa agcagccatt cgccctgata
    13681 cggtattagc ctcagtaatg ttggtgaata atgaaattgg cgtcattcaa gacattgaag
    13741 ccatcggtaa aatttgccgc gagcatggcg tgatttttca tgtagatgcc gcgcaagcca
    13801 ccggcaaagt tgccattgat ttagaaaaac tgcccgttga cctcatgagt tttagcgcgc
    13861 acaaaactta cggccccaaa ggcattggcg cattgtatgt gcgccgtaaa ccgcgtattc
    13921 gcattgaggc acaaatgcat ggcggcggtc atgagcgcgg catgcgctct ggcacattgg
    13981 ccacacacca aattgttggc atgggcgaag cgtttcgtat tgcgcgtgaa gaaatggaaa
    14041 tggaaaacgc acggattcgt aaattgcgtg accgcctact aacaggtttg caaagtattg
    14101 aagaaaccta tgtcaatggc gagttggacc atagaatccc tcataattta aatatcagct
    14161 ttaattatgt ggaaggtgaa tcgttaatta tggccgtgaa aggcattgca gtttcaagcg
    14221 gctcggcttg tacttcggcc agtttagaac ccagctatgt attgcgtgcg ctaggccgta
    14281 gtgatgaatt agcgcatagc tcgattcgtt tttcaatcgg ccgtttcacc accgaggccg
    14341 atgtagatta caccattgcg ctattgaaag aaaaaattag caaattgcgt gaactatccc
    14401 cactttggga aatgtacaaa gatggtattg atatctcaaa agtacaatgg gcggcgcatt
    14461 aaaccgtgaa aagagaaagg tgaaatgtga aacgtaatca aattggcgcg gcgcgcgatt
    14521 tctttcacct ttcgcgtttc aaatttcact aagaattaag gagaactaaa atggcatatt
    14581 cagataaggt tttagaccac tacgaaaacc cgc
//
LOCUS       DAOQSI010000034        14557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_3210_length_14557_cov_5.772031, whole genome shotgun sequence.
ACCESSION   DAOQSI010000034 DAOQSI010000000
VERSION     DAOQSI010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 14557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14557
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3210_length_14557_cov_5.772031"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="PKL42_03700"
     CDS             complement(<1..843)
                     /locus_tag="PKL42_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNU66209.1"
                     /translation="MQAISHIVAATDFSPAAERAIYRAALIAKQLNANLSLIHVAHPL
                     DLYVGTELSFGSQKQIAHMQQSTNISQLSVIADNLKKDFDIPIEIATRIGLAHAEIAS
                     YAASKAGCLIVAGARGQHENAILNLLLGSTATRLLRSANCPVLIVKNKKVSPYQQVIA
                     AVDFSEGSRAVPTIACTLAPEATVETLHVFDVTQEARMRQIGLDDEKLHLYQNSALME
                     VGKQLDKILAKLSDNRVTSKVVTGYPASGICVRATELPADLIVIGRHGMSGFQEWLLG
                     SVSKD"
     gene            complement(917..3613)
                     /locus_tag="PKL42_03705"
     CDS             complement(917..3613)
                     /locus_tag="PKL42_03705"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HNU66210.1"
                     /translation="MKILLHHWHTLSADDVALQLETNHQTGLNAKQVAERIVRHGPNA
                     LQEKRRRSPWRMLLDQFTEFMIMVLIVAAIISGFLGDVGDTLFIIVIVILNAIVGFIQ
                     EFRAERAMAALKRMSEASARVLRDGQVKTINASELVPGDVVLLEAGNVVPADFRLIEA
                     AQLRIDESALTGESVAVEKQIHPLSVVDAPLGDKTCLAYKGTVVTYGRGRGLVIATGM
                     QSELGKIARLLSEGDDSKTPLQKRLAGFGKWISLAALTICVLVFVIGITRGEPLLLMF
                     MTAVSLAVAAIPSALPAVVTISLALGAHKMVKQHALIQSLPAVETLGSVTFICSDKTG
                     TLTQNKMHVTTLYVDGALCSTWQNKNTLIEHESLIKNKDKPWLTLFQALALSNDAHLD
                     SLGKIQGEPTEAAFLRAAKEADWDKVILEQNYPRLKELAFDSERKRMTTFHQTADSVI
                     AYTKGSPEALISQCTRALTSSGEQAIDQLALLQQAEAMAANGLRVLAFAYRHWPALPD
                     SQQPDELEQNLVFIGLIGLIDPPRSEAKEAVSLCKSAGITPVMITGDHPATARAIAHQ
                     LGILADSDGRVMTGADLASLDEKTFKAEVERVRVYARVDPAQKIKIVRALQDKGEIVA
                     MTGDGVNDAPALKAADIGIAMGKGGTDVAREASRMVLLDDNFATIVNAVRYGRRLYDN
                     IRKFVRYAVTTNSAEVLTIFLAPFLGLPIPLLPTHILWINLVTDGLPALALTAEPAER
                     GVMRRPPRAPQESLFAHGMWQHVIWVGLLMAGLTLFVQAWAYHTGSSHWQTMAFTVLT
                     LSQLAHVMAIRSEKESLFSIGIFSNMPLVITVAVTFMLQMATIYVPAFNAIFKAEPLS
                     MHELALCLVLSSVVFIAVEIEKWLIRQGWLYRETKARIATHH"
     gene            complement(3693..4694)
                     /locus_tag="PKL42_03710"
     CDS             complement(3693..4694)
                     /locus_tag="PKL42_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HNU66211.1"
                     /translation="MFLWLQFLTCLITIGFAGYYLSRYGDIIAEKTGMSASWVGLILL
                     ATATSLPELATGISAVSLANAPNIAVGNALGSTVFNLGFLVILDALYQRETLYSRAAQ
                     GHILSASLGALMIAFAGFSLLLDHAGISPALGHVGLYAPFFVLVYFVAIRAVHRYERR
                     TLDAYTEASTERYPQVTLRQAIAGYAMAAMAVVAAGSWLPFVAKDIAELMGWGQSFVG
                     TLLVAAVTSAPEIVVTISALRIGALDMAIANLLDSNLFNIIYLAVDDLFYTKGPLLAS
                     VDAGHAMTAFTAVMMSALVIVGITFRPQHRAVLKLTWISLGLFLLYILNTWIQFQHG"
     gene            complement(4842..5459)
                     /locus_tag="PKL42_03715"
     CDS             complement(4842..5459)
                     /locus_tag="PKL42_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNU66212.1"
                     /translation="MNANSTTIFIVDDDPAIRDALSLMIDLDNVAVQTYESAEAFLTH
                     YQPECRGCAIIDIRMPGMSGLDLHKAMLARHILLPVIFLTGHGDIPMSVQAMKAGAVD
                     FLTKPVTREKLLASVKAAIQKSERVLSENSQHLEALSRLAELTDRERDVMLLAVQGHT
                     NKHIARELGISHRTVEIHKSKIMHKTGATNLLDLARIVHESDLMT"
     gene            complement(5512..7008)
                     /locus_tag="PKL42_03720"
     CDS             complement(5512..7008)
                     /locus_tag="PKL42_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNU66213.1"
                     /translation="MKDLIKHLGFEAIFEAAADAMLLVNNAGIILQANASALSLLEYS
                     AEAMCGLAVEALIPVSYRNHHQHQRQSYFNKPEKRAMGKDKALFALTGSGKKIAVDIS
                     LSPVTIKDQVYVLTTFYVAGHRREAEDALRISEERLQLARRSAGLGVFDFDAKQNIVH
                     WDERMRELWGGDSDSTISYEKFIEAIHPQDRETRQAALILATDPDGTGEYNAEFRIIN
                     AKDGTEHWIATTGRMHFEDNHATRLVGIAQDITERKKLETKLQEQRSETESIFKQQVA
                     ARTASAIAHELNQPLAAISAYSEVALHALSDANFNTDKLKRALEGCVAQAQRAGTSLH
                     ELLAFLQAGNIVVAPFDLNMLVKDALNIARSDGYKEFQPALELEQNLPQVLANRIQIQ
                     KVLVNLIRNAVEAMRSIGTNNSTITVTVRTNKNLNMGHVSVQDSGPGIPAEAVKRIFE
                     PFFTTKPTGIGMGLAISRALVESNGGQLWLEPNVELGAIFHFTLPFAP"
     gene            7276..7521
                     /locus_tag="PKL42_03725"
     CDS             7276..7521
                     /locus_tag="PKL42_03725"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HNU66214.1"
                     /translation="MQLSKVTQLNANNPIPVYGVEAKEKKQVKAKAESKKTEVAVDGQ
                     EMSCRVAMCAYFKAEARGFEPGYELEDWLAAEAEVGK"
     gene            7518..7955
                     /locus_tag="PKL42_03730"
     CDS             7518..7955
                     /locus_tag="PKL42_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66215.1"
                     /translation="MRSAQVVQLQGQVRRRGSFVAVSAEHASLCPSEKVTAVLTLDEN
                     GTIFDCNQAAAKLIDCVPSRLKWKHISKVIPEFAETALVQGGIVNPNLRFLSHIGYQF
                     ELVGLNGVHIACALFFNEVEDFGRHCLRMIIRPVTEENQLVFE"
     gene            7975..8451
                     /locus_tag="PKL42_03735"
     CDS             7975..8451
                     /locus_tag="PKL42_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66216.1"
                     /translation="MVHLIKKRFIVTAAVLCGFVLPQYGSASALMPTQAQNESGVTYI
                     TGGIAEEAEMMRSVAKNYTLEMAFVQKLKQQEEFISEVTVKLINAKKDVVLDTITKGP
                     YLYVNVPSGRYTIVAEFNGVEKHQQVVVNAKKHQKVVFWWPITELPQPEDRSEPIE"
     gene            8509..8823
                     /locus_tag="PKL42_03740"
     CDS             8509..8823
                     /locus_tag="PKL42_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HNU66217.1"
                     /translation="MDLSNKNASKDQLVSDFKVVVADAEALLKATASQGGEKVAEVRA
                     KAEKSLHAAKIALAEAQSVVVEKSKEAAKATDVYVHDHPWKSIGFAASVGLVVGLLIG
                     RK"
     gene            8848..9228
                     /locus_tag="PKL42_03745"
     CDS             8848..9228
                     /locus_tag="PKL42_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNU66218.1"
                     /translation="MAEDSRGLFDSLTAFASTLVSIAHTRLALLSNDLEEDRARLFSL
                     VLLYVAAMFFLIVGVVLAIILVVYILWESHRLLALSSLAGLFLLLGVGLCITALRKSK
                     TKPKLFSSSLSELVKDKDRLDAHE"
     gene            9221..9511
                     /locus_tag="PKL42_03750"
     CDS             9221..9511
                     /locus_tag="PKL42_03750"
                     /inference="COORDINATES: protein motif:HMM:NF025363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HNU66219.1"
                     /translation="MNKKLKVLADRRERLLRDSDLQREQLSGAVNGLRAPLALVDQGV
                     ALVGFFKKHPVLLASSSALMFKVLRPSRLGKWLSRGLFAWQMVRKLHSKFLA"
     gene            9522..10088
                     /locus_tag="PKL42_03755"
     CDS             9522..10088
                     /locus_tag="PKL42_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNU66220.1"
                     /translation="MDIAPAGHRTCLHQSEQLEAVLDQMAWQAAGYLAGKQRVMIVGI
                     LRRGAPLAQMLHNRLVRKFDLAHVELMHLQIKRYADDLTLLHPETQLTENPMQATLNL
                     AHTSVLVVDDVMYHGHSMLRAVEYLARKQALEIRTAVLVDRGANKLPVRVNITGVRLD
                     VAPSDIIECNVPPYEASFKIDLLKPSIK"
     gene            10100..10729
                     /locus_tag="PKL42_03760"
     CDS             10100..10729
                     /locus_tag="PKL42_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNU66221.1"
                     /translation="MFYDREDAARQLAKKLEHLRGKNPLVLAIPRGAVNMAKIIADKL
                     SGDYDVVLVRKLRAPYQPELAIGSVDESGWTYIADYAASVGADNAYIENEKNFQLRTI
                     QQRRAIYTPIRAPLAVEGRVVVVVDDGLATGASMIAALHSLKIKHTARLICAVPVSPP
                     STLNKIAQYADEVVCLASPDDFQAVGQFYLSFPQVDDDEVMEILKAPVE"
     gene            10773..11219
                     /locus_tag="PKL42_03765"
     CDS             10773..11219
                     /locus_tag="PKL42_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66222.1"
                     /translation="MKIKLLTAAMLFSALFSTAHAADVGVSVSIGQPGFYGRIDIGDY
                     PYPPPRVIYRQPRIVERVYIEREPIYLRVPPGHAKNWPKFCGRYHACGEPVYFVQDNW
                     YNNDYAPRYREYHHDDRHEAYDDSKHDKHEYKRQNKGKSKGKSHKH"
     gene            11299..12252
                     /locus_tag="PKL42_03770"
     CDS             11299..12252
                     /locus_tag="PKL42_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HNU66223.1"
                     /translation="MKFKDYYEILGVSRTATSDEIKKAYRKLAHQYHPDISKDPAGEE
                     KFKEIGEAYATLKDSEKRAAYDALGSHPSGQEFRPPPNWSGSQGGQSYSFDDIDLSDL
                     FANFTGRRQGEQHHGGHRPIPGQDYELAIQISLEDAYHGTMVDLNFTVPEYDNQGQLK
                     RVAHTFKARIPKGVSHGQKLLLRGKGGKGANGGKNGNLYLNIGFIAHPLFRVAEHDLL
                     IDLPVTVWEATLGATVKVPTLDGPVNLKIPAGMNSGKKLRIKDRGLPKQNQAHGDLFA
                     VVQIVVSPNPSEQEIALMKQVAEISSFNPRVHFGLDTTNEK"
     gene            12242..12574
                     /locus_tag="PKL42_03775"
     CDS             12242..12574
                     /locus_tag="PKL42_03775"
                     /inference="COORDINATES: protein motif:HMM:NF024979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HNU66224.1"
                     /translation="MKNDDAELLWLDEQHEVTASELLELSGLSAEELEWLVEAGAIKP
                     IRRVPAAVTQQSNAWCFSGQCLIAVRMVSRLKRDFDLAPDALSLTLVLLERIRMLEQQ
                     LQQKNSQA"
     gene            12833..13495
                     /locus_tag="PKL42_03780"
     CDS             12833..13495
                     /locus_tag="PKL42_03780"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNU66225.1"
                     /translation="MQTFTYQRQKIYDPILRLIHLWNGLAIVFLMATIWLSELFEKGA
                     GEDTLWQLHIYIGYALVVGVVARLMWGFVGPRHARFSDMWHPAAWWGAVRHVNFKATP
                     RFGHNTLASGAYLLVYGLLVIMAVTGLGLAAIEHSAGPFNAWLGDTAWLEDFFEEPHE
                     IIYYFLMGFVVIHIAALIWHETQDKTPLAQAMVTGYQYEIEANPSTMTEVSHYIQGEH
                     HA"
     gene            13488..13895
                     /locus_tag="PKL42_03785"
     CDS             13488..13895
                     /locus_tag="PKL42_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1924 domain-containing protein"
                     /protein_id="HNU66226.1"
                     /translation="MRKSMLIIGLLLSFKTFAATPQELLKQYEAQAKQEHSAFAGFSA
                     EKGATFFKTERLHSDGKKVSCATCHTADPRNQGKTRANKVIEPMAPIVNPKRFTDRAK
                     VEKWFGRNCRDVLERACTAQEKGDYVAYMLSIQ"
     gene            13908..14420
                     /locus_tag="PKL42_03790"
     CDS             13908..14420
                     /locus_tag="PKL42_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diheme cytochrome c"
                     /protein_id="HNU66227.1"
                     /translation="MKNWMQLLLLFTSISATSALLASGGMEEGGGAMPVVSNAKWAAE
                     CSSCHMLYHPGLLPERSWRKMMLGLDKHFGENASLDAATQKEITAFLALNSADKQDNR
                     RAKRINQSIPANSTPLRITETRYFTDKHDEVSANTFKRKSIGSASNCAACHKNAEKGN
                     FSESQIKIPR"
     gene            14430..>14557
                     /locus_tag="PKL42_03795"
     CDS             14430..>14557
                     /locus_tag="PKL42_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HNU66228.1"
                     /translation="MRILLVEDDALLGDGVRAGLKQAGFAVDWSQDGQAAKLALET"
BASE COUNT         3961 a         3116 c         3537 g         3943 t
ORIGIN      
        1 atctttgctg acgctaccta atagccattc ttgaaaaccg ctcatgccat gccggccaat
       61 cacaatcagg tcggcaggta attcggtagc gcgcacgcag attccagatg ctgggtagcc
      121 agtcacgact ttacttgtaa cacgattgtc acttagtttc gccagtattt tatcaagctg
      181 tttgcccact tccatgagcg cactattttg gtatagatga agtttttcat catcaaggcc
      241 tatttgacgc atacgagctt cctgtgtcac gtcaaagaca tgtagtgttt cgacagtagc
      301 ttctggcgcc aaagtgcagg cgattgttgg aaccgctctc gatccttctg aaaaatcaac
      361 ggcagcaatt acctgttgat agggtgaaac tttcttgttt ttaacaatca gtactgggca
      421 gttagctgac cttagtaagc gtgtagccgt ggaacctaat agtaggttaa ggatagcatt
      481 ttcatgctgc cctctcgcgc ccgccacaat caggcagcct gctttgcttg cggcgtagct
      541 tgcgatttct gcgtgagcaa gaccaattcg cgtcgctatt tctattggta tatcgaagtc
      601 ttttttgaga ttgtcagcaa taacgcttag ttgactgatg ttagttgatt gttgcatgtg
      661 tgcaatttgc ttttgagacc caaaagacaa ctctgttccc acatataaat ctaagggatg
      721 tgctacgtga ataaggctca aatttgcgtt gagttgtttt gcaatgagtg cggcacgata
      781 aatggctcgt tcggcggcag gcgaaaaatc ggtggcggca acaatgtgag aaatagcttg
      841 catgacgttc tcctgataat tgaagtatat ttttttttaa atatgatgac tatgaggtaa
      901 atactaaaag gtcatatcaa tgatgagtgg caatccgcgc ttttgtttca cggtacaacc
      961 aaccttgccg tatcaaccat ttttcaattt ctactgcaat aaatacaaca ctagataaaa
     1021 caagacaaag agcaagctcg tgcatactca gcggttcggc tttaaaaatg gcgttaaatg
     1081 caggcacgta tatggttgcc atttgtaaca taaacgtcac agcaacggtg attaccaatg
     1141 gcatgttcga gaaaatgccg atgctaaata gcgactcttt ttcagagcgt attgccatga
     1201 catgcgctaa ttgagataac gttaacacag taaatgccat ggtttgccag tgagaagacc
     1261 ctgtgtggta tgcccatgcc tgtacaaata gggttagccc cgccatgagc agacctaccc
     1321 aaatgacgtg ctgccacatc ccatgtgcaa acaagctttc ttgtggtgcg cgtggcggac
     1381 ggcgcattac accgcgttcg gcaggttcgg cggtcagcgc caaagccggt aagccgtcag
     1441 tgaccagatt aatccacaat atatgggttg gtagcaatgg gattggcaac cctaaaaaag
     1501 gcgccaagaa aatcgtcaat acttctgccg agtttgtggt gactgcgtag cgaacaaatt
     1561 tacgaatatt gtcatacagg cgcctaccgt agcggacagc attgactatt gtggcaaagt
     1621 tatcgtctaa caacaccatg cgcgatgctt cacgtgcgac atcggtgccg cccttgccca
     1681 tggcaatgcc aatgtctgcg gctttaagtg ctggcgcatc gttgacacca tcgcctgtca
     1741 tggcgacaat ctcgccttta tcttgcagcg cgcgtacgat tttaattttt tgggctgggt
     1801 ccacgcgcgc ataaacgcgt acgcgttcaa cttcagcttt aaatgttttt tcatctaaac
     1861 tggccaaatc ggcgccagtc attactctgc catcgctatc agccaatata ccaagttgat
     1921 gcgcgatggc acgcgcggtg gcagggtggt ctcccgtaat catcacaggc gtaatgccgg
     1981 cggatttgca taatgaaacc gcctcttttg cctcgctacg tggcgggtca atcaagccga
     2041 ttagcccaat aaacaccagg ttttgttcca actcatcagg ctgctggctg tcaggcagtg
     2101 caggccaatg acgataggca aacgcaagca cgcgtagtcc attggctgcc attgcttcag
     2161 cctgttgcag caatgctaat tggtctatgg cttgctcacc acttgaagtg agtgcgcgcg
     2221 tacattgtga tatcagcgcc tctggcgacc ctttagtgta agcaatcacg ctatccgctg
     2281 tctgatgaaa agtagtcata cgcttacgct cagaatcaaa cgcgagctct tttagccttg
     2341 gataattttg ttctaatatc actttgtccc agtcggcttc tttggcagca cgcaaaaaag
     2401 cagcttctgt cggctcgcct tgtatttttc cgaggctatc aagatgcgcg tcattactca
     2461 atgctaacgc ctgaaacaag gtaagccacg gtttatcttt gttcttaatc aaagattcgt
     2521 gttcaatcaa agtgttctta ttctgccatg tgctacaaag cgcgccatct acatacaagg
     2581 tggttacatg cattttattc tgcgtcagtg tgccagtttt atcagagcag ataaaagtca
     2641 ctgagcctaa cgtttcaacg gctggcaagc tttgaatcaa ggcatgctgc ttgaccattt
     2701 tatgcgcgcc aagcgcgagt gaaatcgtca ccacggccgg caatgccgat ggaatggccg
     2761 ctacagccaa actcactgct gtcataaaca tcagcaataa gggctcgcct cgggtaatac
     2821 caatgacaaa caccagtacg caaatcgtca gcgcagcaag tgatatccac ttaccaaagc
     2881 cagccaagcg tttttgtaat ggcgttttgc tgtcgtcacc ttcgcttagt aatctggcga
     2941 ttttacccaa ttcactttgc atgcccgtgg cgatgactaa gccacgtccg cgtccgtaag
     3001 tcacgactgt acctttgtaa gctaggcagg ttttgtcgcc caaaggagca tccactacag
     3061 ataggggatg aatttgtttt tcaaccgcga ctgattcacc agtcagcgct gattcatcaa
     3121 ttctgagttg tgccgcctca atcagtctga aatcggctgg caccacgttg ccagcttcca
     3181 gcagtacaac atcgcctggc accagttcgg atgcgttaat ggtctttact tggccatcgc
     3241 gtagcacacg tgcgcttgct tcagacatgc gcttgagagc tgccattgcg cgctctgcac
     3301 gaaactcttg gataaaacca acaatcgcat tcagaatcac aatcacgatg ataaataaag
     3361 tatcgccaac atcgcccaaa aatcctgaaa tgatggccgc aacaatgagc accataatca
     3421 tgaattcggt aaattgatcg agcagcatgc gccaaggtga acgcctgcgc ttttcttgca
     3481 gggcattagg gccgtgccgt acgattcgtt ctgccacttg tttagcgttt aggccggttt
     3541 ggtgattcgt ttcaagttgc agcgccacat catctgctga tagtgtgtgc caatggtgca
     3601 gaaggatttt catatgatca ataaacggcg gtattgcttg aaaaaatcca actaagtaat
     3661 ggcgtcatgg taagtacctg atatctattt tattacccat gctgaaattg tatccatgtg
     3721 ttaagaatgt acaacagaaa caggcccaga ctaatccaag tgagttttag tacagcacgg
     3781 tgctgcgggc gaaaagtaat tccaacgatt accagcgcac tcatcatcac tgcagtaaag
     3841 gccgtcatgg cgtgaccggc atctacgctg gcaagtaaag ggcctttggt gtaaaacaag
     3901 tcatctaccg ctaggtaaat aatgttaaac aaattgctgt ccaacaagtt ggcaatcgcc
     3961 atatccagcg cgccgatacg taatgcagat atggtaacca cgatttccgg tgcggaggtg
     4021 accgcagcca ctaacaaagt gccaacgaag ctttgtcccc agcccatcag ttcggcaatg
     4081 tctttagcta cgaaaggcag ccaggaacct gctgctacca ctgccattgc cgccattgca
     4141 taaccggcga ttgcttgacg caatgtcact tgcggatagc gttctgtcga ggcttcggta
     4201 taggcgtcca gcgtgcgccg ctcatagcgg tgaacagcac ggatggctac aaaataaact
     4261 agcacaaaaa atggtgcata caacccaacg tggccgagcg cggggcttat accagcatgg
     4321 tcgagtaata ggctaaatcc tgcaaaagca atcatcagtg cgcccagcga ggcagacaaa
     4381 atatgtcctt gtgcggcgcg gctgtatagc gtttcgcgct gatatagcgc atctagtatc
     4441 accagaaagc caagattaaa tacggtgcta cccaatgcgt tgccgactgc gatattgggg
     4501 gcattggcca gtgagactgc ggaaattcca gtcgccaatt ccggcaaaga agtagcggtg
     4561 gctagcaaga tgaggcctac ccaagaggcc gacatccccg ttttttcagc aatgatgtca
     4621 ccatagcgtg aaaggtaata gcctgcaaaa ccaatggtaa tcaaacaggt gagaaattgt
     4681 agccaaagaa acaatggatt gaccttacct tatagtgtta ttgcatactg atcgttcgct
     4741 ttggttttta tctgacatca aagcacttat ttagggttga ttttaccgcc atcgaaattt
     4801 gcttgctacg cgtgtgaaca cgtagcttca tcttgcaaga cttaagtcat taaatcactt
     4861 tcatgcacaa tgcgcgcaag gtctagcaag tttgtagccc ctgttttatg cataatttta
     4921 gatttgtgaa tttctaccgt gcgatggcta atgcctaatt cacgggcaat atgcttattt
     4981 gtatggcctt gcacagcaag tagcatcaca tcacgctctc tgtcggtaag ctcagcaagt
     5041 cgcgacaagg cttctagatg ctgagagttt tccgataaca cccgttcaga cttttgtatt
     5101 gccgctttta ctgaggctaa aagcttttcg cgtgttacag gtttggtaag aaagtctact
     5161 gcgcctgctt tcatggcctg cacgctcatt ggaatgtcgc catggccggt gaggaaaatg
     5221 accggtaata atatgtggcg tgccaacatc gctttatgca aatccagacc actcatgcca
     5281 ggcatgcgaa tatcaattat ggcgcagccg cggcactctg gctgataatg ggttaaaaat
     5341 gcttcagcgc tctcataagt ttgtacagca acgttgtcca aatcaatcat cagagacagc
     5401 gcgtcacgaa tagcggggtc atcatccaca ataaaaatcg tcgtgctgtt ggcgttcata
     5461 ggattgtatt ttccgagtgt tttaaaagtt aatgaaccga tattgcatat tttatggtgc
     5521 aaaaggtaat gtaaagtgaa aaatagcgcc taattctacg ttaggttcca gccatagctg
     5581 cccaccgttc gattcgacta aagcacggct aatggcaagt cccattccga ttcctgtagg
     5641 cttggtggta aaaaaaggct cgaatattcg cttcaccgct tctgcgggta tgcctggccc
     5701 actatcctgc actgatacat gccccatatt aaggttttta ttcgtgcgta ccgttacggt
     5761 aatggttgag ttgttggtgc caatgctacg catggcttcc acggcattac gaattaaatt
     5821 aaccagtact ttttgtattt gtatacgatt tgctaatacc tgaggcaaat tttgctcgag
     5881 ttccagtgcc ggttgaaatt ctttataacc gtcagatctg gcaatattca gtgcatcttt
     5941 cacaagcata tttaaatcaa agggtgcaac cacaatgttg ccagcttgca aaaaggcaag
     6001 taattcatgc aaactggttc ctgcacgttg cgcttgggca acacaaccct caagtgcacg
     6061 ctttagttta tccgtgttga aattagcatc actcagtgca tggagggcaa cttcactgta
     6121 agctgaaatg gcagctaagg gttgatttag ctcgtgtgca atcgcagagg ccgttcttgc
     6181 tgcgacttgt tgtttaaata tagattctgt ttctgaacgt tgttcttgta atttagtttc
     6241 aagcttttta cgttcagtaa tgtcttgtgc aatacccacc agtctcgtgg catgattgtc
     6301 ctcaaagtgc atgcgccctg tcgtggcaat ccaatgttct gtgccatctt ttgcattaat
     6361 gatgcgaaat tcagcattat attcccctgt gccatcgggg tcggtggcta aaattaaggc
     6421 ggcttgtctg gtttcacggt cttgaggatg aatcgcttca ataaattttt cgtatgaaat
     6481 tgtgctgtct gaatctccac cccacaattc gcgcatgcgc tcgtcccaat gcacgatatt
     6541 ctgcttggca tcaaaatcaa aaacccctaa tccagcagaa cgcctcgcca gttgtaaacg
     6601 ctcttcactg attcttaacg cgtcttcagc ttcacggcga tgaccagcca cataaaaagt
     6661 agtgagtacg taaacttggt ctttaatagt gacgggactc aaacttatat ccacggcaat
     6721 ttttttgcca gaacccgtta aagcgaaaag cgctttatct ttgcccatcg cgcgcttttc
     6781 gggcttgtta aaataagatt ggcgctgatg ttggtgatga ttgcgatagc tgacaggaat
     6841 aagtgcctca actgcaaggc cgcacatcgc ttcggcagag tactcaagta gcgatagcgc
     6901 cgaggcattg gcttgaagaa taatgcccgc attgtttacc aataacatgg catctgcagc
     6961 ggcttcaaaa atcgcttcaa aacccaggtg tttgattaaa tctttcaatg gatttacagc
     7021 ctaattattt ggtttatcgt gttggaatct agcaacgcag tctagtaaaa aagttaaatg
     7081 atgtccaatc attacagatg cattctccag tttttgatat aagggcttgc ccttacgtgc
     7141 tagcccgaat ttacgtagag ggtttagtcc agtacattgc aagtgtcgat ggtgcttaac
     7201 ttgcaacggc tagttgcact aatcccccta gatgagtgcc gtcgacattt tttggtgcaa
     7261 gcgaggagaa atgtgatgca actttcaaaa gttacacagc ttaacgcaaa taacccaatt
     7321 cctgtgtatg gggttgaagc caaagaaaaa aaacaagtta aagccaaggc tgaaagtaag
     7381 aaaacagaag tggcggtgga cggtcaagag atgagttgtc gtgttgcgat gtgcgcttac
     7441 tttaaagcgg aggcgcgagg ttttgagccc ggctatgagt tagaagattg gctagcggca
     7501 gaggctgagg tggggaaatg aggtccgcgc aagtggtgca gttgcaaggt caagttaggc
     7561 ggcgtggtag ttttgtggct gtaagtgctg aacatgcgag cttatgccca agtgagaagg
     7621 tgacagccgt actcacgctg gatgaaaatg gcaccatttt tgactgtaac caagcggcgg
     7681 caaagttgat tgattgtgta ccgagcaggc ttaaatggaa gcatatctcc aaagtgattc
     7741 cagagtttgc ggaaacggct ttggtgcaag gcggtattgt taacccaaat ctgcgatttt
     7801 tatcgcatat cggttatcaa tttgaattgg tgggtttaaa cggtgtacat attgcttgtg
     7861 cgctattttt taatgaagtt gaagattttg gtcggcattg tttaagaatg attattcgac
     7921 cagtaacaga agaaaatcaa cttgtatttg agtgaagttg caaaaaggaa aaaaatggtt
     7981 catttgatta aaaagcgttt tattgttaca gcagccgtgc tgtgtggttt tgtgttacct
     8041 cagtatggta gtgcttcggc cttaatgccg acgcaagcgc aaaatgaaag tggcgtgacc
     8101 tatatcactg gcggtattgc tgaagaagct gagatgatgc ggagtgtcgc taaaaactac
     8161 acattagaaa tggcgtttgt acaaaagctt aaacaacaag aagaatttat ctctgaagta
     8221 accgttaagc tcatcaacgc caaaaaagac gttgtactgg acaccattac caaagggcct
     8281 tatttatatg tgaatgtgcc gtctgggcgt tacacaattg ttgccgagtt taatggggta
     8341 gaaaaacacc agcaggtcgt ggtcaatgct aaaaaacatc aaaaagtagt attttggtgg
     8401 cctattactg agttgccgca gccggaagat cgttcagagc ccattgaata aaagtagttg
     8461 aactataacg agattgaata aaagaaaact aacaaaagga gaataaaaat ggatttgtct
     8521 aacaaaaatg caagtaaaga ccaattggtg agtgatttta aagtggtggt ggctgatgcc
     8581 gaagcgctgt tgaaagctac tgcgagccaa gggggtgaaa aagtggcgga agtgcgtgca
     8641 aaagcagaaa aatcactcca tgccgcaaaa attgccctag ctgaagcgca atctgtggtg
     8701 gttgaaaaat caaaagaagc ggcaaaagct actgatgttt atgtgcatga ccatccttgg
     8761 aaatcaattg gttttgctgc cagcgtgggc ttggtggttg gcttgcttat tggccgtaaa
     8821 tagtttattt gctgacgagg cgattaaatg gctgaggaca gcagggggtt gtttgactcg
     8881 ctgaccgcgt ttgcaagtac tttagtcagt attgcgcata cgcggttggc gttgttatcg
     8941 aatgatttag aagaagatcg cgcccgttta ttttcgttgg ttttgctcta tgttgccgca
     9001 atgttttttc tgattgtggg cgtggtgctc gccattattt tagtggtcta catcttgtgg
     9061 gaaagtcatc gattgttggc gctttcttct ttagcaggcc tatttttgct gttaggcgtt
     9121 ggcctttgca tcactgcatt gcgaaaatcc aaaacaaaac ctaaattgtt ttcatcaagt
     9181 ttgtctgagt tggttaaaga taaagatagg ctagatgcgc atgaataaga aactcaaagt
     9241 attggctgat aggcgtgagc gattacttcg tgattctgat ttgcagcgcg agcaactctc
     9301 cggcgctgtc aatggattac gcgcaccgct tgcgttggta gaccaagggg tggcgttggt
     9361 tggttttttt aagaagcacc cagtattact tgcaagcagc agtgcgctga tgtttaaagt
     9421 attgcggcca agtcggctgg gaaaatggct aagtcgcgga ttatttgctt ggcagatggt
     9481 gcgtaagcta cattctaagt ttttggctta ggggcaaagc gttggatatt gcgccggctg
     9541 gtcatcgcac ttgcttacat caaagtgagc aattggaggc cgtattagat caaatggctt
     9601 ggcaagcggc agggtatttg gcaggaaaac agcgcgtcat gattgttgga attttacgac
     9661 gcggcgcgcc tttagcgcaa atgctgcata acaggctggt gcgtaagttt gatttggcgc
     9721 atgttgaatt gatgcacttg caaattaagc gctatgcaga tgatttaact ttacttcacc
     9781 cagaaaccca gctgactgaa aacccgatgc aggcaacatt gaatctcgca catacttcgg
     9841 tgctggtggt ggatgatgtg atgtatcacg ggcattccat gctgcgtgca gtggaatatt
     9901 tagccagaaa gcaggcgttg gaaattagaa ctgcggtgct ggttgaccgc ggtgcgaata
     9961 aactgcctgt gcgggtaaat attacaggcg tgagactgga tgtggcaccg tcagacatca
    10021 ttgagtgcaa tgtgccgcct tacgaagcaa gttttaaaat tgacttgctg aagcctagta
    10081 tcaaataggt atctgagtaa tgttttatga ccgtgaagat gcagcccgcc agttagctaa
    10141 aaaactagag catttgcgtg gcaaaaatcc gctcgtgttg gcgattcccc gaggtgctgt
    10201 caacatggcg aaaatcatcg ccgacaaact aagtggtgat tacgatgttg tgctggtgcg
    10261 taagttacgc gcgccctatc agccggaatt agccattggc tcagtggacg aaagtggttg
    10321 gacctatatt gcagattatg ctgcttccgt tggcgctgat aatgcctata tagagaatga
    10381 aaaaaatttt caactgcgta caattcaaca acgccgcgct atttatacac caatacgtgc
    10441 gccactggcg gtggaaggac gtgtggttgt ggtagtggat gatgggttgg ccacgggcgc
    10501 cagtatgatt gcagcattgc atagtttgaa aatcaaacat acagcgcgac tgatttgtgc
    10561 tgttcctgtg tcgccaccga gcactttaaa taaaatcgcc caatatgcag atgaagtcgt
    10621 ttgtttggcg tcacccgatg attttcaggc ggtaggccag ttttacctga gttttccgca
    10681 agtggatgac gatgaagtga tggagatttt aaaagcgcct gtagaatagg gttgttggat
    10741 tttatagata gaatagttga aaaagttgaa tgatgaaaat taaattatta acagcagcaa
    10801 tgttgttttc ggcattgttt agtacggcac atgcggccga cgtgggcgtt tcggtcagta
    10861 ttggtcaacc tgggttttac gggcgaattg atattggcga ttatccttac ccgcctccgc
    10921 gcgttattta tcgccagcct agaattgttg agcgcgtgta cattgaacgc gagcctattt
    10981 atttgcgcgt tccgccgggc catgccaaaa attggcccaa gttttgcggc cgatatcatg
    11041 cgtgcggtga acctgtctat tttgttcagg ataactggta taacaatgat tatgcaccgc
    11101 gttaccgtga atatcatcat gatgacaggc atgaagccta tgacgactca aaacatgaca
    11161 agcatgaata taaacgtcaa aacaaaggta aatcaaaagg gaaaagtcac aagcattaaa
    11221 gcgatgagag gcatcgtcat ctttaaggct atgctttagc gttatgaatt taacttacaa
    11281 tcgctcaaca aaccaaacat gaaatttaaa gattattacg aaattcttgg tgtatcgcgg
    11341 acggcaacaa gtgatgagat taaaaaagct tatcgaaaac tggcgcacca gtatcaccca
    11401 gatatatcaa aagaccctgc gggagaagaa aaatttaaag aaatcggtga agcgtatgcc
    11461 acattaaaag acagcgaaaa acgtgcagct tatgacgcgt taggcagtca cccatccggc
    11521 caggaattca ggccgccgcc aaattggtca ggcagtcagg gtggccagag ttattcattt
    11581 gacgacatcg atttatctga tttatttgca aattttactg gcaggcggca gggtgagcag
    11641 caccacggcg ggcataggcc aataccaggt caggattatg aacttgccat acagatttca
    11701 ctggaagacg cctatcacgg cacgatggtt gatttgaatt tcaccgtacc tgaatatgac
    11761 aatcaaggtc aactcaaacg cgttgcacat acttttaaag caaggattcc taagggcgtg
    11821 agccatggcc aaaagttgct actccgaggt aaaggtggca aaggcgcaaa tggcggtaaa
    11881 aacggcaact tatatttaaa tatcggcttc attgcgcatc cattatttcg tgtggctgag
    11941 catgatctgt taattgattt gccggttacc gtttgggaag ccactttagg cgcaactgta
    12001 aaagtgccca ctttagatgg gccagtaaat cttaaaatac cagctggtat gaatagcggc
    12061 aaaaaattgc gcatcaaaga ccgtggcttg cccaagcaaa atcaagcaca tggtgatctt
    12121 tttgctgtgg tacaaattgt tgtttcgccc aacccgtctg aacaagaaat tgcattgatg
    12181 aaacaggtgg cggaaatttc aagttttaat ccacgcgtgc attttggatt ggatactaca
    12241 aatgaaaaat gatgacgcag aattgttatg gctagatgag cagcatgaag tcacagcaag
    12301 cgaactctta gagctttctg gcttatctgc agaagagctg gaatggctgg tagaagccgg
    12361 tgccattaaa cctattcgac gtgttccggc agcggtgacg cagcaaagta atgcgtggtg
    12421 ctttagtggt caatgcctta ttgccgtcag aatggtgtca agactgaaga gagattttga
    12481 tttggcaccc gatgcgttga gtttgacctt ggtgcttcta gagcgcattc gaatgctcga
    12541 acaacagtta cagcaaaaaa actcgcaagc ataaagtact tgggcatagt atttttaatc
    12601 tttatttaat aaaaaatact tctttaaaat cctttaattt aaacacaacc tttttcgatg
    12661 ccctgcgagc ctttatttat aaggctcgca gggcattatt tttttctcgg acgattttcg
    12721 tctatttttt catgtttttt ttcgcattca aaaaaaacat ttttccaatt aaggttatgt
    12781 taagaatcgc gttctaaaat gcattcacct ttaaataaaa aggagtgcaa aaatgcaaac
    12841 attcacgtat cagcgccaaa aaatttacga cccaatatta cgtttgattc acctttggaa
    12901 tgggctggcg attgtttttt taatggcaac aatttggttg tcagagctgt ttgaaaaagg
    12961 cgcaggtgaa gacactttat ggcagttgca tatttatatt ggctacgcct tagtagtggg
    13021 tgtggtcgcg cgcttgatgt ggggctttgt ggggccgcgc catgcgaggt tttcagatat
    13081 gtggcaccca gccgcgtggt ggggtgcggt acgccatgtt aactttaaag ccacaccgcg
    13141 ctttgggcat aacacgctcg ccagtggtgc ttatttgctc gtgtatggct tgcttgtcat
    13201 catggctgtg actggtttag ggcttgccgc cattgagcat agtgctgggc cgttcaatgc
    13261 ttggttgggt gacacggctt ggttagaaga cttttttgaa gagccacacg aaattattta
    13321 ctacttttta atgggctttg tcgtcatcca tatcgccgcg cttatctggc atgaaacaca
    13381 agacaaaaca ccactggcgc aagcgatggt gacaggctat caatatgaaa ttgaagccaa
    13441 tccatcaaca atgactgaag tcagtcatta cattcaagga gaacatcatg cgtaagtcaa
    13501 tgttaattat cggacttttg ttaagtttta aaacatttgc cgctacacct caagaactac
    13561 taaagcagta tgaagcgcaa gccaaacagg agcattcggc atttgcaggg ttttctgcag
    13621 aaaaaggcgc tactttcttt aaaacagaac gcttacatag cgatggcaaa aaggtgagtt
    13681 gcgccacttg ccacacggct gacccgcgca accaaggcaa aacacgcgcg aataaagtca
    13741 ttgagccgat ggcgccaatt gttaacccga aacgctttac cgatagagca aaagtggaaa
    13801 aatggtttgg ccgcaattgt agggatgtgc tggagcgcgc atgtaccgcg caagaaaaag
    13861 gcgattacgt tgcctacatg ttaagcattc aataaaggag aatcatcatg aaaaactgga
    13921 tgcaattact gttacttttc acgagtatca gcgcaacaag cgccttactc gccagtggcg
    13981 gaatggaaga aggcggtgga gcaatgcctg tggtgagcaa tgcaaaatgg gcggcagagt
    14041 gcagcagttg ccatatgtta taccatccgg gcttattgcc agagcggtct tggcgcaaaa
    14101 tgatgctggg cctagataaa cattttggtg aaaacgcaag tctagatgca gcaacgcaaa
    14161 aagagattac cgcttttctt gctttgaata gtgccgataa acaagataac cgacgcgcta
    14221 agcgcattaa tcagtcgatt ccagccaatt caacgccatt acgcattact gaaacacgct
    14281 actttactga taagcatgat gaagtttctg ccaacacgtt taagcgtaaa agcatcggca
    14341 gtgcatcaaa ttgcgcggcg tgtcataaaa atgcagaaaa aggtaatttt tcagagtcgc
    14401 agattaagat tccgcgctaa tataaagcca tgcgaatact gttagttgaa gatgatgccc
    14461 tgttgggcga tggtgtgcgg gcaggtttaa agcaggccgg ctttgcggtg gattggtcgc
    14521 aagatggcca agcggcaaag ttggcactag aaactga
//
LOCUS       DAOQSI010000035        14397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_3262_length_14397_cov_6.197880, whole genome shotgun sequence.
ACCESSION   DAOQSI010000035 DAOQSI010000000
VERSION     DAOQSI010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 14397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14397
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3262_length_14397_cov_6.197880"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PKL42_03800"
     CDS             complement(<1..390)
                     /locus_tag="PKL42_03800"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNU66229.1"
                     /translation="MRKKLVVANWKMHGNLALNQQLISAYLAQLSTLNEVDVVVCVPY
                     PYLFQAQQILQNTSIAWGAQNISKAEVGAFTGEVSAAMLNDFGASYVIIGHSERATAY
                     CESDENIATKFIQAQKHGITPILCVGET"
     gene            complement(484..1707)
                     /locus_tag="PKL42_03805"
     CDS             complement(484..1707)
                     /locus_tag="PKL42_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNU66230.1"
                     /translation="MLLRVLLLMVTLSFSFAGCANEAKTMPVPKSVANAIQLPEGFKL
                     EVFADFTKHPPLTRGAPRMMALDAAGNLYVSMPRQGKVVMLARNQPENQAVLVADNLN
                     APHGLVFVGEKLFVAHQDGIVVLEKDPQATSASGWPAKTVTGLVKNLPTGGHTLKTLK
                     LGPDGLLYINVGSSCNVCNESDPLRATILRYTTDGKAAGALATFGRHAPNPIFASGLR
                     NSQGFAWHPQSQAMFATNNGADNRSEIKNGPVNDELPPEHLNLIAAGKHYGWPYCWGV
                     AKDSQGMYSDPNFAGEADFCKSATPPAITFVSHSTPMGITFLDKTNWPAEYKHDAIVA
                     LHGSWNRKTPSGYKLVRVRFDGDKPIAVSDFATGWLHGNAAFGRPVDVIVGADGALYV
                     SDDRADVIYRISSKQ"
     gene            complement(1948..2394)
                     /locus_tag="PKL42_03810"
     CDS             complement(1948..2394)
                     /locus_tag="PKL42_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HNU66231.1"
                     /translation="MKKIMLSLCCMAVMLVTNVVIASETTPAGLWKTIDDKTGKPRSL
                     IRITENNGEYSAVVEKGLLATDTGEAVCDKCTDDRKGQKIVGMTIVKGIKKKNDSYEG
                     GEILDPDNGKTYKCKMKLDSTGTQLEVRGFIGVSLFGRSQTWIREE"
     gene            2625..2840
                     /locus_tag="PKL42_03815"
     CDS             2625..2840
                     /locus_tag="PKL42_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66232.1"
                     /translation="MKKQFGISTLELLCSLTIMSSITAYTVAMSEEVETAIVDYQKTT
                     NVKNIKEKIHTASCLPQNNKESESAEN"
     gene            complement(3031..4308)
                     /locus_tag="PKL42_03820"
     CDS             complement(3031..4308)
                     /locus_tag="PKL42_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNU66233.1"
                     /translation="MNKNLTLTRRNFIKAGAATGLLLANPAFSMGRKPPLGRVVIVGG
                     GYAGTTAAKYIRMWSLGAIEVVVVEKSSQFVSCPMSNLVLGGTKTIDDLTFGYDLVQK
                     NHGIQWAQDEVTAINTQAKKVIMKRGELNYDRLIVAPGIDFDYAALPNLMTKDAQQQI
                     PHAWKAGWQTVNLRKQLEQMPDGGVFVMTIPKAPYRCPPGPYERACQVASYFKAHKPN
                     SKVIVLDANAEVISKKGLFQKAWKALYPNIVEYRPNSAIVSVDVATKTVITELDQVKA
                     DVLNVIPPQQAGKVAQLANLLEPDYPWCEVDFLTYESKVAPNVHVIGDSVSSGLPKSA
                     HMATSQARVCASAIVELMKGQSPDPAPVFANTCYSFIDDKLAMHVANVYRYDAPKKIM
                     VPAEGGGVSANASAQEGVYAQAWAQNIWADVLT"
     gene            complement(4301..4711)
                     /locus_tag="PKL42_03825"
     CDS             complement(4301..4711)
                     /locus_tag="PKL42_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66234.1"
                     /translation="MHHNKLQKIFIALIASSVIWPLLAQTAEDVVVSAAQNSDLHARV
                     LAASCAACHGTNGNAVTGSNENRNAILAGRDANDFTTIMLGFKNGSREATVMHHHAKG
                     LTEDEIRQLAVYFSKQKPTGAVLLKPQTLKAYHE"
     gene            complement(4790..5287)
                     /locus_tag="PKL42_03830"
     CDS             complement(4790..5287)
                     /locus_tag="PKL42_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNU66235.1"
                     /translation="MKLISAMLAVLALSFSMNAMALSDDDTLEFVNAITEGNLKVVKK
                     FVEAEPADVNYKSFAWSPIQMAANKGQIEVAKYLISKGAELDYVHPLSKNTAFHLAAF
                     GNHREMATLLAKSGADVNKKLKANVSLLRPFQDANNTDMIAFLTGLGVKDDGCKDVKC
                     FEEPD"
     gene            complement(5372..9304)
                     /gene="purL"
                     /locus_tag="PKL42_03835"
                     /gene_synonym="purI"
     CDS             complement(5372..9304)
                     /gene="purL"
                     /locus_tag="PKL42_03835"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HNU66236.1"
                     /translation="MSQSNANPQMLCLRGSAALSQFRIDKIQSQLSHHSNIKHLYAEF
                     RHFIWVENKGAEVALSAAQQETLKQILTYGPTLQVEDPQGELLVVIPRIGTISPWASR
                     ATDIVQHCGLENVQRVERGIAYFVQTKNGEKLTQDERKALLPLIHDRMTETVIANLDD
                     AAKLYHTDTPKPLSTVDILQGGKAALVQANSEMGLALSPDEVDYLFENFSKIQRNPTD
                     VELMMFAQANSEHCRHKIFNADWVIDGVQQAFSLFGMIRNTHKRNPGNTVVAYSDNSS
                     IVEGQNVGGKTKRFYPREDGAYGFIEEEMHYLMKVETHNHPTAISPFAGAATGAGGEI
                     RDEGATGSGSKPKAGLTGFSVSNLNIPNFEQPWESNDYGRPSRIASPLQIMIDGPLGG
                     AAYNNEFGRPNIAGYFRTFELEANNEVRGYHKPIMLAGGLGNISAKHSKKNPIPAGAA
                     LVQLGGPAMLIGLGGSAASSMDTGSNVENLDFDSVQRGNPELERRAQEVIDRCWQLGD
                     KNPILSIHDVGAGGISNAFPELVNDAGVGAVFQLRDVHNEEPGMSPRELWSNEAQERY
                     VMAIAQEDLPRFKEICERERCPFAVVGVATEERHLTVADSYFGNKPVDMEMSVLLGKP
                     PKMTRDVKSIHKTFTEFDTSKIDLKDAAARVLRLPGVADKTFLITIGDRSVTGLVARE
                     QMVGPWQVPVADVAVTLAGYETYVGEAFAIGEKAPLALINAPASGRMAIGEAITNIAA
                     CLIDDLSNLKLSANWMAPAGHAGEDAALYETVKAVGIELCPQLGVSIPVGKDSMSMKT
                     VWDDKGEKKAVTSPISLVVTAFATTPDARKTLTPQLKTDVATKLILIDLGAGKNRLGG
                     SSLAQVYGAVGDVAPDVDDALLLKRFFNTIQSLNKASKLLAYHDRSDGGLLTTLVEMA
                     FAGGCGLNVDISALQGDAVSALYNEELGAVIQVSATEANEVLGQLKLALSSNAYIIGE
                     VAAGNSIEIAQHGKVIFSDTRVNLHRIWSETTYHMQSLRDNPVCAQQEYDRILDEQDR
                     GLFAELTFDPSENIAAPYISTGTRPKMAILREQGVNGHVEMAVAFDRAGFATFDVHMS
                     DIIAGRVSLQDFAGFVACGGFSYGDVLGAGEGWAKSILFNNRARDEFSAFFNRADSFA
                     LGVCNGCQMMSNLHSIIPGAQHWPHFVKNKSEQFEARLAMVEVLESPSLFFSGMAGSK
                     MPIAVAHGEGFTEFSGESAVNDVLAKNLVTMRFIDSASTPTVRYPFNPNGSPQGITGL
                     TSTDGRFSIMMPHPERVIRNVQNTWQRCGDAEDGAWLRMFRNARKYIA"
     gene            complement(9829..10509)
                     /locus_tag="PKL42_03840"
     CDS             complement(9829..10509)
                     /locus_tag="PKL42_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66237.1"
                     /translation="METPLICNHGCPTWFQGSMSDLNKMFMQGKWLMSSKNFSVKTQR
                     FYSAHHTLLRIAKDNLRRAETEGKGCANWQFAAITLSSLAIEAICNAVGEKVVGNWSD
                     FESCSPIAKTRLICEHLSINYDTSKEPWASFVWLCKTRNLIAHPKAEPIMYEAVITEQ
                     EHDSLEFRHAPKSKLEKRMTLSNAKKSVAVVDQLINLFCEKLTSEQKFGISGDMWHSS
                     STIQNDIN"
     gene            complement(10535..11248)
                     /locus_tag="PKL42_03845"
     CDS             complement(10535..11248)
                     /locus_tag="PKL42_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HNU66238.1"
                     /translation="MHQFFTKSFITKNSLATLGFIFFATQANALGVSDLTNTEASGGL
                     KEALTQGVGKAISTLGAADGFLGNKEVKIPLPSSLKKIEKGMKLMGMGKQSDELILKM
                     NRAAEAAVPEAKTLLVDSIKKMTVADAKAILTGPNDAATQYFKKTTSEQMAAKFLPIV
                     EKATANVQLADSYNKYAEMGSKFGVVKKEDANINQYVTKKALDGVYLMIAKEEAAIRQ
                     DPVGQASNLLKKVFGALGK"
     gene            11486..12049
                     /locus_tag="PKL42_03850"
     CDS             11486..12049
                     /locus_tag="PKL42_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66239.1"
                     /translation="MKNYLKTKTCIIILALSSIPSMAAASPNSLLYISANDYSHSVRL
                     LHPYYDYWFEQGPLVEPIAFEALKEKLPDISMCKANETADTIIRIKPSLFYNPQMRVY
                     HGRLVATVFSGGGNVLGTYVGEAQQQGFLSVNHATQLHIQKVYAKAMQNLMSKFEIKP
                     IERNGNAENKLPCGLVGAQEEAKINFY"
     gene            complement(12282..>14397)
                     /locus_tag="PKL42_03855"
     CDS             complement(12282..>14397)
                     /locus_tag="PKL42_03855"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNU66240.1"
                     /translation="IETDAALGNGGLGRLAACFVDSMATMDIPATGYGIRYEYGMFRQ
                     SIENGQQVENPDNWLRYGNIWEFQRPEATYNIRFYGHVVKYADEHGETQHWVDGEHVV
                     AMAYDVPVPGYGTETVNSLRLWSAKAAREFDLRHFNDGDYEKAVAERNETESISKVLY
                     PNDTSVLGKELRLKQQYFFVSASIQDILRRFLSTNEMKKQADWKLLPEKVAIQLNDTH
                     PSIGVAEMMYQLVDVHGLPWAFAWELTGKIFAYTNHTLMPEALETWSVDLFGRLLPRH
                     LEIIYKINHEFLHMVNHHFPGDVDLLARVSIIDESHGRRVRMAHLAVVGSHTVNGVAA
                     LHSELLKQHLFADFDRIFPGKLTNVTNGITPRRWLNQANPGLTALIEKAIGAGFKKDL
                     TQIKKITPLANDAAFRKAFAAVKQANKARLAAKIEQKTGVKLNVNSLFDVQIKRIHEY
                     KRQLLNVLHVITLYNRIRRGEKTTPRTVIFGGKAAPGYWMAKQIIRLINDVAQIINDD
                     PHVGDQLKVVFYPNYEVSAAEILFPGSDLSEQISTAGTEASGTGNMKMALNGALTIGT
                     LDGANVEILEEVGHENIFIFGLTTPQVAEIKTNGYHPRAFYESNPALKEVIDMIANGF
                     FSVEEPNRYQAIVDNLLNNDQYLLLADYASYIETQDKVSSVYQDQDQWTRMAILNVAN
                     MAKFSSDRSISDYANNIWKVKV"
BASE COUNT         3888 a         3479 c         2964 g         4066 t
ORIGIN      
        1 cgtctctccc acacacaaaa ttggcgtgat gccgtgtttt tgggcttgaa taaacttggt
       61 tgcgatgttt tcatcggact cacaataagc ggtagcgcgc tcggaatgcc caataattac
      121 atagcttgcg ccaaaatcat tgagcattgc tgcactgact tcgccggtaa acgcgcctac
      181 ttcagccttg cttatatttt gcgcacccca ggcaatagag gtgttttgta atatttgctg
      241 tgcttgaaaa aggtatgggt acggcacgca gaccaccaca tcaacttcat tgagggtaga
      301 taattgcgct agataagccg atataagctg ttgatttaac gctaagttgc catgcatttt
      361 ccaattggca acaactagtt tttttcgcat gctaaccttg tttaaaattg attcggcgta
      421 attttacgct gaagttgaag gttgctgtca aattacgcat caaaattgtg cgacattgaa
      481 acattattgc ttactgctaa tacggtaaat gacatcggcc ctatcatccg acacataaag
      541 cgcgccgtca gcgccaacaa tcacatccac cgggcggcca aaagctgcat ttccatggag
      601 ccagcctgtc gcaaaatctg acaccgcaat cggcttatcg ccatcaaagc gcacgcgcac
      661 caatttgtag ccgcttgggg tttttctatt ccaagagcca tgtaacgcaa caatggcatc
      721 atgtttatat tctgctggcc aattggtttt atctaaaaaa gtgatgccca ttggcgttga
      781 atgtgacaca aaagttatgg cgggtggcgt ggcagacttg caaaaatctg cttcaccggc
      841 aaagtttgga tctgaataca tgccttggga atctttggct acgccccaac aataaggcca
      901 gccgtaatgc ttacctgccg cgataagatt taaatgctct gggggaagtt catcattgac
      961 gggcccattt ttaatttctg aacgattatc tgcgccatta ttggtcgcaa acatcgcttg
     1021 agattgcgga tgccaggcaa atccttggga atttcgcaaa cctgatgcga aaattggatt
     1081 aggcgcatgc cgaccgaatg tcgcaagcgc acccgctgcc ttaccatcag tggtgtaacg
     1141 tagaatcgtt gcacgcaatg ggtcactttc gttacagaca ttgcaacttg aaccaacatt
     1201 aatatacagc aaaccatcag gccctaattt caaagtttta agggtgtggc cacctgttgg
     1261 taagtttttc acgaggccgg tcacggtttt ggccggccag ccactagcag aggttgcttg
     1321 cgggtctttt tctaacacca caataccatc ctgatgagcg acaaacagct tttcgccaac
     1381 aaaaaccaag ccatgcggag cgtttaaatt gtcagccacc agcactgcct gattttcagg
     1441 ctgattacgg gccaacatca ccaccttacc ctgcctaggc atgcttacat ataaatttcc
     1501 agccgcatca agcgccatca tgcggggtgc gccgcgtgtt aatggtggat gttttgtaaa
     1561 gtcggcaaac acttctaatt tgaagccttc tggcagttga attgcattcg ccacactttt
     1621 aggcacaggc atcgttttag cttcattggc acatccagcg aaactaaaac tcaatgtcac
     1681 catgagcaac aaaacacgta acagcatcat aaactcctca attaaaaaaa cgtatttttt
     1741 aactaaattt accccaacct ttttcgatgc tctgtgagcc ttataaataa aggctcacag
     1801 agcatatttt ttctctcggc gaatttttac gcattttaca aggtatttaa aacctgtttg
     1861 gcaagcattt tttaggcatc aaacgaatgc attaacttaa aaatgccgca aaatttaagt
     1921 gcgcttgatg cgatgatttt ttgtgaatta ttcttcgcgt atccaagttt gagagcgccc
     1981 aaacaatgaa acgccaataa aaccgcgcac ttctaattgt gtaccggtac tatcgagctt
     2041 cattttgcat ttataggttt tgccgttatc cgggtctaaa atctccccgc cttcgtagct
     2101 gtcatttttt ttcttaatgc ctttcacaat ggtcatgcca acaatttttt gacctttacg
     2161 atcatccgta catttgtcac ataccgcttc gcctgtgtct gtcgccagca agcccttttc
     2221 aaccacggcg ctatattcac cattattttc ggtgattctg attaaagagc gcggtttgcc
     2281 cgttttatca tcaatggttt tccataatcc tgccggcgta gtttcagaag cgattacaac
     2341 attggtcacc agcatcacgg ccatacagca caagcttaac attatttttt tcatccagaa
     2401 tctccttaaa tgttttaatt caaccagtta accaccaaca aaacccccaa acatgactgc
     2461 gaataacatt cacttagtca atatcagtgc gtcaatatac aacaaaatgc gctgcttaaa
     2521 aaaaatctcg gacacaaatt tacacaattc actaaagctt ttagaaattg tgccgttgat
     2581 taacttgtag attcattaaa agcaataaaa gacaggatta cgctatgaaa aaacaattcg
     2641 gaatttcaac cttagagttg ctctgttcac tcaccattat gtcatcaatt acggcataca
     2701 cggtggcgat gtctgaggaa gtggaaactg cgattgtgga ctaccaaaaa acaaccaatg
     2761 tgaaaaatat caaagaaaaa attcacaccg ccagctgcct gccacaaaac aataaagaaa
     2821 gtgaatctgc tgaaaattaa tacgttacgc cagaaaaaat atcgcaattt ttaagcgcaa
     2881 aacatcgtta aaaagcttaa aaaatataag ccttctccct ccctgccctg ccagcctcta
     2941 tttacaaggc tcccagggca gctttttttg ctcgggagaa tttatagcct taaaaacgtt
     3001 aaagcttaaa aacgtttaag ataagttgtt ttatgtcagc acatctgccc aaatattttg
     3061 cgcccaggcc tgtgcataaa ctccttcttg tgcacttgca tttgctgata cgccgccacc
     3121 ttcagcaggc accatgattt tttttggtgc gtcatagcga tacacattcg ccacgtgcat
     3181 cgcaagcttg tcatcaataa agctatagca agtattggca aatacggggg cggggtctgg
     3241 gctttgccct ttcatgagct ctacaattgc gcttgcacac acacgcgcct gactggtcgc
     3301 catatgcgca gattttggca agccagatga aacactgtca ccaataacat gcacattggg
     3361 cgcaactttt gattcatagg tgagaaagtc aacctcacac catggataat ccggttccag
     3421 caagttggct aactgcgcaa ctttaccggc ctgttgtggt ggaataacgt tcaacacatc
     3481 tgcttttact tggtctagct ctgttatcac agtttttgtg gcaacatcaa cgcttacaat
     3541 cgcgctattt ggacgatact ccacaatgtt cggatataac gctttccatg ccttttgaaa
     3601 caagcctttt tttgaaatga cttccgcatt ggcgtctaac acaatcacct tgctatttgg
     3661 tttgtgtgct ttaaagtagc tcgccacttg gcaagctctt tcgtatgggc caggcgggca
     3721 acgatacggg gctttaggaa tggtcataac aaacacgccg ccgtcaggca tttgttcaag
     3781 ttgcttacgt aaatttacgg tttgccaacc cgctttccat gcatgcggta tttgctgctg
     3841 ggcgtcttta gtcattaaat ttggcaaggc agcataatca aaatcgatac caggcgcgac
     3901 aatcaaacgg tcatagttaa gttcaccacg cttcatgatg actttttttg cttgggtgtt
     3961 aattgcagtg acttcgtctt gcgcccattg aatgccatga tttttttgta ctaaatcata
     4021 accaaaagtt aaatcatcaa tcgttttagt gccgcccaac accaaattac tcatcgggca
     4081 tgaaacaaac tggctggact tttcgaccac caccacttca atcgccccca aactccacat
     4141 gcgaatatat ttggccgccg tggtgccggc atagccaccg ccaacaatca ccacgcggcc
     4201 cagtggcggt tttcgcccca tcgaaaaagc tggattcgcc agcaacaagc cagtggcagc
     4261 gccggctttg atgaaattac gtcgggtcaa cgttaggttt ttattcatga taggctttca
     4321 aagtttgcgg cttcaaaagc accgcgcctg tcggtttttg ctttgaaaaa tacacggcga
     4381 gctgcctgat ttcatcttca gtcaagcctt ttgcgtgatg atgcatcacc gtcgcttcgc
     4441 ggctgccatt tttaaagcca agcataattg tggtgaaatc attggcatct cgccccgcga
     4501 gaatcgcgtt tctattctcg ttgctacccg tgactgcatt gccattggtg ccatgacatg
     4561 ctgcacacga ggcagccaac actcttgcat gtaagtctga attttgtgct gcagaaacaa
     4621 caacatcctc agcagtttgt gccaataaag gccaaatcac tgaggatgcg atgagtgcta
     4681 tgaaaatttt ttgaagttta ttgtgatgca tgatgttcac ctgaattcaa tgaacatcat
     4741 tctaaacgtt taatacaaat tattcgtgac caaagtcaca tattgccgat taatcaggtt
     4801 cttcaaagca tttaacgtct ttacagccgt catctttaac acctaaccct gttaaaaacg
     4861 caatcatatc ggtgttattg gcatcttgaa atgggcgtaa tagtgaaaca ttggctttta
     4921 gttttttgtt cacatcagcg ccgctttttg ccagcaaagt ggccatttct ctgtggttgc
     4981 caaaggccgc caaatgaaat gctgtgtttt tagagagcgg atgcacgtaa tcaagctctg
     5041 cacctttact gattaaatat tttgccactt caatttggcc tttattagcg gccatttgaa
     5101 taggagacca agcaaatgat ttgtaattca cgtcagcagg ttcagcctca acaaattttt
     5161 tgaccacttt gagattgccc tcggtaatgg cattcacaaa ttccagcgta tcatcatcag
     5221 ataacgccat cgcgttcatt gaaaaactta aggctagcac cgccaacatt gcactaatta
     5281 atttcatatt tctttatctc caagttaatt gacttacaac actacatact acctaatcat
     5341 cttacctagc catcctacca aaatcatgcg attaagcgat atattttcta gcatttctaa
     5401 acattcttaa ccaagcgcca tcttcagcat caccacagcg ttgccatgtg ttctgtacat
     5461 tgcgaatgac acgttcagga tgtggcatca taatactaaa acgtccatct gtgctggtta
     5521 aacctgtaat cccttgcggc gaaccgtttg gattaaacgg gtaacgaacc gttggcgttg
     5581 acgcactatc aataaagcgc attgtcacta aatttttagc cagcacgtca ttgaccgcgc
     5641 tttcaccaga aaattccgta aagccttcac catgtgcaac cgcaattggc attttactgc
     5701 ctgccatgcc gctaaagaac aaagatggcg attccaatac ctcgaccatc gccaatctgg
     5761 cttcaaattg ctcagattta ttttttacaa aatgcggcca atgttgcgcg ccaggaataa
     5821 tgctgtgtaa attactcatc atttggcaac cgttacatac gccaagtgca aagctatcag
     5881 cacggttaaa aaatgcagag aattcatcgc gcgcgcggtt gttaaataaa atagacttcg
     5941 cccaaccttc acccgcgccc agcacatcac cgtaagaaaa cccgccacaa gcgacaaagc
     6001 ccgcaaaatc ttgcaatgaa acacggccag caataatatc gctcatgtgt acgtcaaacg
     6061 ttgcaaaacc tgcacgatca aatgcaacag ccatttcaac atggccattc acaccctgct
     6121 cacgcagtat ggccattttt gggcgtgtac ccgttgaaat ataaggtgca gcaatatttt
     6181 cacttgggtc aaacgtcagt tcagcaaata aaccgcggtc ttgctcgtct aaaatacggt
     6241 catattcctg ctgcgcacat actgggttgt cgcgcagact ttgcatgtgg taggtggttt
     6301 cagaccagat gcgatgtaaa tttacacgcg tatcgctaaa aatcactttg ccatgctgcg
     6361 caatttcaat gctgttgcca gccgccactt caccaatgat gtaagcatta gagcttaatg
     6421 cgagtttgag ttgccctaaa acttcgttag cttctgtagc actcacttga atcaccgcgc
     6481 caagctcttc gttataaagt gcacttacag catcgccctg caacgctgaa atatcgacat
     6541 ttaagccgca acccccagca aaggccattt caaccaaagt agtcaataaa ccaccatctg
     6601 aacggtcgtg atacgcaagc agcttgctag ctttgtttaa actttgaata gtattaaaaa
     6661 aacgtttgag gagcaaagca tcatccacat cgggcgccac atcacccacc gcgccataca
     6721 cttgcgccaa acttgagccg cccaagcggt ttttgcctgc gcctaagtca attaaaatca
     6781 gcttagttgc aacgtcggtt tttaattgtg gcgtcagcgt tttacgtgca tctggcgtgg
     6841 tggcaaatgc cgtcaccacc aacgaaatcg gtgaagtgac cgctttcttt tcacctttat
     6901 catcccatac cgtcttcatt gacatggaat ctttacccac ggggatgctc acacccaatt
     6961 gcgggcatag ctcgatgccg actgccttga ccgtttcata caaagcagca tcttcccctg
     7021 catggcctgc tggcgccatc caattggcag agagtttcaa gttacttaaa tcatcaatta
     7081 aacaggcggc aatattggta atggcctcgc ctatcgccat gcgacctgat gctggcgcgt
     7141 tgattaaggc taacggtgct ttttcaccaa tggcaaaggc ttcacccaca taggtttcat
     7201 agcctgccag cgtcacggcc acatccgcca cgggcacttg ccatgggccg accatctgct
     7261 cgcgcgcgac taagcctgtg actgagcggt cgccaatagt aatcaagaaa gttttatccg
     7321 ccacacccgg caagcgcaac acgcgcgcgg cggcgtcttt taaatcaatt ttgctggtat
     7381 caaactcagt aaaggttttg tgaatgcttt tcacatcgcg cgtcattttt ggcggcttac
     7441 caagtaacac tgacatttcc atatcaacgg gtttattgcc aaaatagcta tctgccaccg
     7501 ttaagtgacg ttcttcagtc gccacgccca ccacggcaaa cgggcaacgt tcgcgctcgc
     7561 aaatttcttt aaagcgcggt aaatcttctt gtgcaatcgc catcacataa cgctcttgcg
     7621 cttcgttact ccacaattcg cgtggtgaca tgcctggttc ttcattatgc acatcgcgca
     7681 attgaaacac tgcaccaacg cccgcatcgt tcaccaattc aggaaaagca tttgaaatac
     7741 cgcccgcgcc cacatcatga atgctcaaaa tagggttttt gtcaccgagt tgccagcaac
     7801 ggtcgatgac ttcttgcgca cggcgctcta attctgggtt gccgcgttgt acggaatcaa
     7861 aatctaaatt ctcgacgttt gagccggtgt ccatactcga agccgcgcta ccgccaaggc
     7921 caatcaacat cgctgggccg cccagttgca ctaacgccgc acctgctgga atcggattct
     7981 ttttagagtg tttggcagaa atgttgccca atccacccgc caacataatc ggcttatgat
     8041 agccgcgcac ttcattattt gcttctaatt caaatgtacg gaaataaccc gcgatattcg
     8101 ggcggccaaa ttcattgtta tacgccgcgc cacccaaagg gccatcgatc ataatttgca
     8161 ggggtgaagc aatacggctt gggcgaccgt aatcattcga ttcccacggc tgttcaaaat
     8221 ttgggatatt taagttcgat actgaaaacc ccgtcaaacc cgctttcggt ttagagccgc
     8281 tgcccgttgc gccttcatcg cgaatctcac cgcccgcacc tgtggcagcc cctgcaaaag
     8341 gtgaaatcgc tgtcgggtga ttatgcgttt ctaccttcat taaataatgc atttcttctt
     8401 caataaaacc atatgcgcca tcttcacgcg ggtaaaaacg cttggttttt ccgcctacgt
     8461 tttggccttc aacaatcgaa gaattgtcag aatacgccac cactgtattg ccggggttgc
     8521 gtttatgtgt gttgcgtatc atgccgaaca gtgaaaacgc ttgctgcacg ccatcaatca
     8581 cccaatcagc attaaagatt ttgtggcgac aatgctcaga gttcgcctgt gcgaacatca
     8641 tcaactcaac gtcagtgggg ttacgctgaa ttttgctaaa gttttcaaac agataatcca
     8701 cttcatcggg tgacagcgcc aaacccatct cactattggc ctgcaccaat gcggccttgc
     8761 caccttgcag aatatccact gtgcttagtg gcttgggcgt atcagtgtgg tacaacttgg
     8821 ccgcatcatc caagttcgca atgaccgttt cagtcatgcg gtcatgaatc agcggcaaca
     8881 acgctttacg ctcgtcttgc gttaattttt caccattttt agtttgcaca aaatacgcaa
     8941 taccgcgctc gacccgttgc acattctcta agccgcaatg ctgcacaata tcggttgcgc
     9001 gtgatgccca aggtgaaatg gtgccaatgc gcggaatcac caccagcaat tcgccttgcg
     9061 ggtcttcaac ttgcaaggtc ggcccgtaag tcaaaatctg ctttagcgtc tcttgttgcg
     9121 cggcgcttaa ggcaacctcc gcgcccttgt tttcaaccca aataaaatga cgaaactccg
     9181 catacaaatg cttgatgttc gagtggtgac tcagttgcga ttgaatctta tcaatacgaa
     9241 actgagataa agcagcgctg ccacgcaggc aaagcatttg agggttagcg ttagattggc
     9301 tcatggaata tcgggcaaaa attcaaaggt ttatttaagc cgaaatttta acagattagc
     9361 gccatttcag ccgaaaaaat acaacgaatg acggccataa aaaacgttat ttttgacgca
     9421 ttcaagcccg agaaaaaaca acgactctgc gagcctttat ttataaggct tgcagagcat
     9481 cgaaaaaggt tggtgataga aaagaggttt taaaagaaaa aataagtgta aattcaactg
     9541 caattttgaa tttaaattcc cctgctaccg cccctgatat tgtgctaatg ttgggaggat
     9601 ttcggtaagc ggctgtcaca accgacttag tgctttagca cttagcgagg ttggggaagg
     9661 cccttgcggt cttaagagcg taagtgagtt tccgcttgcc gtccaaaaat tggggcaatt
     9721 taaggaaaca agcgattggg ggtgtcttct tctttggtta ctttcttgtg gacaagcaca
     9781 aaaaagtaac tcgcctagag gagaaaaggt gtattacata ctacgagcct aatttatatc
     9841 attttgaatc gtagaggagg aatgccacat atcgcctgaa ataccaaatt tttgctcact
     9901 agttaatttt tcacagaaca aattaatcag ttgatcaaca actgccacag attttttagc
     9961 attgctcagt gtcattcttt tttctaactt tgatttcgga gcatgtctga attctaggct
    10021 atcatgttct tgttcagtta ttacagcctc atacattatt ggttcagctt tggggtgggc
    10081 tatgagatta cgtgttttgc ataaccaaac aaagcttgcc caaggctctt tactagtgtc
    10141 atagttaata ctcaaatgct cacaaatcaa tctagttttt gcaattggac tacaactttc
    10201 aaaatcactc caattaccta caactttttc tccaacagca ttacatattg cttcaatagc
    10261 tagagagctg agagttattg cagcaaattg ccagttagca caacctttac cttcagtttc
    10321 cgcccttctc agattatctt tagcaatgcg aagtaatgta tggtgtgctg aatagaatcg
    10381 ctgggttttt acactgaaat tttttgaact cattaaccac tttccctgca taaacatctt
    10441 atttaaatca ctcatactcc cctgaaacca cgttgggcag ccgtggttgc aaatcaatgg
    10501 ggtttccatt gatttgcatt ttcaataagc taacttactt acccaatgcc ccaaacactt
    10561 tcttcaacaa attactcgcc tgccccactg ggtcttggcg aatggccgct tcttctttgg
    10621 caatcatcaa atacacgcca tctaaggctt ttttagtcac gtattggtta atattggcgt
    10681 cttctttttt caccacgcca aatttactgc ccatttcagc atatttattg tagctatctg
    10741 ccaactgcac gttggcggtg gctttttcta caatcggtaa aaatttagcg gccatttgct
    10801 ctgaggtggt ttttttaaag tattgcgtgg cggcatcatt tgggccagtt aaaatggctt
    10861 tggcatcggc cacggtcatt tttttaatcg aatccaccag taaggtttta gcttctggca
    10921 cggcagcttc ggcagcgcgg ttcattttta aaatcaattc gtctgattgt ttgcccatgc
    10981 ccattaactt catgcctttt tcaatttttt tgagcgaact aggtaacgga attttcactt
    11041 ctttattgcc caaaaagcca tctgcggcgc cgagcgtgct gatggctttg cccacgcctt
    11101 gggtgagggc ttctttaagg ccgccgctgg cttcggtgtt ggttaaatcg ctcacgccta
    11161 gcgcattggc ttgcgttgcg aaaaaaataa agcctaaagt agctaaacta ttttttgtta
    11221 taaaagattt tgtaaaaaat tgatgcattt ttcttctcca atgaatgagg ttgcaggcgt
    11281 tagcataacc ctaaatatat tcttttgcat gctggtaatg ataatttacg tttgtgcttc
    11341 attcacatcc attcaggcaa agcatgaact tgaacttatc aagtttacag aagccaaaaa
    11401 ttgggtgaaa atcaacgcga gtggttaacg catcagcctg atgccttgtt gacaacgcac
    11461 agtatgtcaa cgattggaga ttactatgaa aaattattta aaaacaaaaa cttgtatcat
    11521 tattttggca ttatccagca tccccagcat ggcggcggcc agcccgaaca gtttgctata
    11581 tattagtgcc aatgactaca gccatagcgt gcgcttgctg cacccatatt atgattattg
    11641 gtttgagcaa gggccgctgg tggagccaat tgcgtttgaa gcgcttaaag aaaaattgcc
    11701 agacatcagt atgtgcaaag ccaacgaaac ggctgatacg attattcgca ttaaacccag
    11761 tttattttac aacccgcaaa tgcgcgttta tcacggcagg cttgttgcaa cggtgttttc
    11821 tggtggtggc aatgtgcttg gcacttacgt gggcgaggcg caacagcagg gatttttaag
    11881 cgtgaaccac gcgacgcaat tgcatattca aaaagtttac gcaaaagcca tgcaaaattt
    11941 aatgtcaaaa tttgaaatta aaccgattga gcgcaacggc aacgccgaaa acaaattgcc
    12001 ttgtggttta gtgggtgcgc aagaagaagc caaaatcaat ttttattgag attttttatg
    12061 agaaaactta tgataaaact aggtgattaa ttttccttaa aaacgcatct aaaaccactt
    12121 aaaatcgccc gagaaaaaac gctgccctgc gagcctttat ttataaggct cgcagggcag
    12181 cgaaaaaggt tgctaatgat tttgacttaa tttaaacgtt taaattaaag catttgaact
    12241 taaaattaaa tccttaaagt gaatcactta aagttaatca cttaaacttt cactttccaa
    12301 atattattcg cgtaatcgct gatggagcgg tcacttgaaa acttggccat attcgccaca
    12361 tttaaaatcg ccatgcgcgt ccattgatct tggtcttggt acacgctgga aactttatct
    12421 tgcgtttcaa tatagctagc gtaatcggcc aatagtaaat attggtcatt attcaataag
    12481 ttatcaacaa tggcttgata gcgattaggc tcttcaaccg agaagaaacc gttcgcaatc
    12541 atatcaatca cttcttttag cgctgggttg ctttcgtaga acgcacgtgg gtggtagccg
    12601 ttggttttaa tttcagccac ttgcggagtg gtcaggccaa aaataaagat gttttcatgg
    12661 ccaacttctt caagaatttc tacgttagcg ccatcaagcg tacctattgt caacgcgccg
    12721 tttaaggcca ttttcatgtt gcccgtgccg ctggcctcgg tgcctgcggt agaaatttgc
    12781 tcagacaaat cactgcccgg gaacagaatt tcagccgctg agacttcgta gttcgggtag
    12841 aacaccactt taagttgatc gcctacgtgc ggatcgtcat tgatgatttg cgccacatcg
    12901 ttaatcaacc gaatgatttg cttcgccatc caatagcccg gcgctgcttt accgccaaaa
    12961 atcacggtgc gcggcgtggt tttttcgcca cggcgaatgc gattgtataa agtaatcacg
    13021 tgtaatacat tgagcaactg gcgtttgtat tcgtgaatac gtttaatttg cacatcaaac
    13081 aagctattta cgttgagttt tacgccggtt ttttgctcga tttttgcggc caagcgggct
    13141 ttattggctt gttttaccgc ggcaaatgct tttctaaacg cggcatcgtt ggctaacggt
    13201 gtgatttttt tgatttgcgt caggtctttt ttaaagcctg cgccaatggc tttttcaatc
    13261 agcgcggtta agcccgggtt ggcctgattt aaccagcggc gcggtgtaat gccgtttgtg
    13321 acattggtta attttcctgg aaaaatgcgg tcaaaatcgg caaataaatg ttgcttcaac
    13381 aactcactgt gcagtgcggc tacaccattc acggtgtgcg aacccaccac tgccaagtgc
    13441 gccatgcgta cacggcggcc gtgcgactca tcaataatcg acacgcgggc aagcaaatca
    13501 acatcgcccg gaaaatgatg attgaccatg tgcaagaact catggttgat tttatagatg
    13561 atttctaaat ggcgcggcaa caggcggccg aacaaatcaa ccgaccatgt ttccagcgct
    13621 tctggcatca aggtgtggtt ggtgtaggcg aaaatcttgc cagtcagttc ccacgcaaat
    13681 gcccatggca aaccatgcac atcaaccaat tgatacatca tttcagccac gccaattgat
    13741 gggtgcgtgt cattgagttg aatggcgact ttttctggca acagtttcca gtcggcctgt
    13801 tttttcattt cattggtgct taaaaagcgg cgcaaaatgt cctgaataga ggctgaaacg
    13861 aagaaatact gctgttttaa acgcagttct ttacccaaaa cagaggtatc gttcgggtac
    13921 aacacttttg atatgctttc tgtttcgtta cgctcggcga cggctttttc atagtcaccg
    13981 tcgttaaagt ggcgcaaatc aaactcgcgc gcggctttag ctgaccataa acgcaggctg
    14041 ttcacggttt ctgtaccata gcctggcacg ggcacatcgt aggccattgc caccacatgt
    14101 tcgccatcta cccaatgctg tgtttcacca tgttcatcgg cgtatttcac cacgtggcca
    14161 taaaagcgga tattgtaagt ggcttcaggg cgctgaaact cccagatatt gccatagcgc
    14221 agccaattat cagggttttc tacttgttga ccattttcaa tactttgtct gaacatacca
    14281 tattcataac ggatgccgta gcctgtcgca ggaatatcca tcgttgccat ggaatcaaca
    14341 aaacaagcgg ccaaacgacc taaaccacca ttgcccagcg cggcatcggt ttctatc
//
LOCUS       DAOQSI010000036        14153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_3355_length_14153_cov_5.386722, whole genome shotgun sequence.
ACCESSION   DAOQSI010000036 DAOQSI010000000
VERSION     DAOQSI010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 14153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14153
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3355_length_14153_cov_5.386722"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..82
                     /locus_tag="PKL42_03860"
     tRNA            6..82
                     /locus_tag="PKL42_03860"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40..42,aa:Val,seq:gac)
     gene            412..2205
                     /gene="lepA"
                     /locus_tag="PKL42_03865"
     CDS             412..2205
                     /gene="lepA"
                     /locus_tag="PKL42_03865"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNU66241.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRIIHLCGGLADREMEAQVLDSM
                     DIERERGITIKAQTAALQYKADDGQIYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQSVANCYTALDLGVEVTAVLNKIDLPSADPERVKQEIEDVIGVDASDAVL
                     CSAKTGLGVRDVLEAVIKKVPPPQGNIDAPLKALVIDAWFDNYVGVVMLVRVVDGVLK
                     PKDKIRLMATHSDFLCEQVGVFTPKSLQKSLLSAGEVGFVIAGIKELSSAKVGDTVTL
                     ANKPAAAPLAGFKEVKPQVFAGLYPVESNQFEALRTALEKLSLNDASLLFEAENSTAL
                     GFGFRCGFLGLLHMEIVQERLEREYDMDLITTAPTVVYEILLKDGSVVQIENPSRLPE
                     PSRVEETREPIIVVNLLMPQDYVGPVMTLCNNKRGIQKNMQYMGRQVMLSYEIPLNEV
                     VLDFFDKLKSVSRGYASMDYEFLEFRAADLVKLDIMVNGERVDALSMIVHRSNSVHRG
                     REVAAKMRELIPRQMFDVAIQASIGANIIARETVKAMRKNVIAKCYGGDVSRKRKLLD
                     KQKEGKKRMKQVGNVEIPQEAFLAILRVEDK"
     gene            2363..3142
                     /gene="lepB"
                     /locus_tag="PKL42_03870"
     CDS             2363..3142
                     /gene="lepB"
                     /locus_tag="PKL42_03870"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNU66242.1"
                     /translation="MPFALFMLLMLVLTGVIWLVDSLFFKAKRENAGEYPEVVEYARS
                     FFPVILVVFFVRSFIVEPFKIPSGSMMPTLLAGDFILVNKFHYGLRVPVLNNTFIEVN
                     HPKRGDVFVFHYPPDPSIDYIKRVVGLPGDKIRYQNKRLTINGQLLELNEVGQYNYVS
                     AGLNMVTATQYREKLGEVSHEILNNDWVGNYEHDSIGAKFANDEEITVPAGHYLAMGD
                     NRDNSSDSRVWGFVPEHNLVGKAFFIWMNFDQGSRIGKSVQ"
     gene            3188..3595
                     /locus_tag="PKL42_03875"
     CDS             3188..3595
                     /locus_tag="PKL42_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HNU66243.1"
                     /translation="MLNKINTQKQFLQSLPVKKQAGATLLGMMFVGGMIVFVALIAMK
                     MFPAYQEYFSVKTIIRAMKQEVGNMSKNEIAASFDRRADTGYVTVIRGKDLEIGKNAS
                     GDTTVTARYRVVTPLFGNVSVLMDFETSTDSKL"
     gene            3619..4311
                     /gene="rnc"
                     /locus_tag="PKL42_03880"
     CDS             3619..4311
                     /gene="rnc"
                     /locus_tag="PKL42_03880"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNU66244.1"
                     /translation="MTLQLLAKRLKHQFEDENLLSQALTHRSYSGKNNERLEFLGDGA
                     LNFIIANQLYQRFQTLDEGDLSRLRAQLVKEATLCEIALSLGLGDALKLGEGELKSAG
                     WRRPSILADALEAIIGATYLDGGFVAAEALVLDLYAEKLSTIDPKVIDKDPKSLLQEF
                     LQSKKIAVPEYAVVQTSGEAHAQIFVVECFIQKLDIRTVGEGTSRRIAEQKAAQLAMQ
                     QVEQLSIRKPKK"
     gene            4339..5232
                     /gene="era"
                     /locus_tag="PKL42_03885"
     CDS             4339..5232
                     /gene="era"
                     /locus_tag="PKL42_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNU66245.1"
                     /translation="MTQTTVGFKCGTVAIVGRPNVGKSTLLNHILGMKLAITSRKAQT
                     TRHRLLGIHTTDDTQFLFVDTPGFQQKHLNTLNKGLNKTVTQVLTEVDVVLFVIEPMK
                     LGEADEKVLALLPKDKPVILVVNKADLMGDKNNLLPLIQDFDLLHPFVGIVPVSAKKS
                     LHLEELLETTRKYLPEQPAVYGEDELTDKNERFLAAELIREKIFRLLGDEVPYAVAVE
                     IEKFEVVGKLRRIFAAIIVDKDSQKPMLIGKNGEKMKQISTEARQDMEKFFGGKVYLE
                     TWVKVQGGWADNARALKSLGY"
     gene            5251..5973
                     /gene="recO"
                     /locus_tag="PKL42_03890"
     CDS             5251..5973
                     /gene="recO"
                     /locus_tag="PKL42_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNU66246.1"
                     /translation="MALASNLRQDNQSVYVLHSYPFKETSLVVELFSQQFGRVAAVAK
                     GARRPRSAMRGMLQSFQLLSGTWSGKNELKTLHSLDWAHGLVNLQGEALMCGFYMNEL
                     LLRLLPREDAHEALFDYYQTTLSTLSKSTDLATHLRRFELKLLQELGYAVPLIADEHG
                     EVISASKQYRYEAEFGARAPHENHNGVQLSGSTLLDMAKDNYQNSLTQAQSKQLMRYL
                     LAHYLGDKPLHTRQLLIDLQGF"
     gene            5975..6703
                     /gene="pdxJ"
                     /locus_tag="PKL42_03895"
     CDS             5975..6703
                     /gene="pdxJ"
                     /locus_tag="PKL42_03895"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNU66247.1"
                     /translation="MIKLGVNIDHVATIRQARGTKYPSVVQAALRAEQSGADSITLHL
                     REDRRHMQDADIFALRPLLQTKMNLECAVTDEMIEIALKVAPQDVCLVPERREERTTE
                     GGLDVIGHFDKIKKAVQRLGDAGIRTSLFIAPDLAQIDAAVKTGAPVVELHTGTFADA
                     HTDALQDVERKRIEIALHHAKDAHLVVNAGHGLHYHNVHHIAKMYGFEELNIGHAIIA
                     HALFVGWDNAVREMKALIKEFSPN"
     gene            6707..7090
                     /gene="acpS"
                     /locus_tag="PKL42_03900"
     CDS             6707..7090
                     /gene="acpS"
                     /locus_tag="PKL42_03900"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNU66248.1"
                     /translation="MIYGIGTDIVEVARIEASINQFGDDLAKRILAESEFKSYMDSNI
                     KPRFLAKRFAAKEAFSKALGTGLRAPATMQNIAVSHDDLGKPILVLANELQAFLQAKD
                     ITKMHISISDEKNLAAAFVVLESTN"
     gene            7246..8043
                     /locus_tag="PKL42_03905"
     CDS             7246..8043
                     /locus_tag="PKL42_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HNU66249.1"
                     /translation="MTALSLDFSRRFGGVARLYGADGLTNLQAAHICVIGIGGVGSWA
                     AEAVARNAVGKITLIDLDNIAESNVNRQLHALDGAFGKAKVTAMAERIKQINPHATVI
                     QIEDFVSPENIATLLNQPYSGVIDCIDDAKAKAAIADFCKKNAIPLVMTGAAGGRLNA
                     THIKQADLSVVSHDKLLAKVRNLLRREYGFAKAISGKTSKFGVLCVYSDESVIKPEGV
                     CDTEDVGITGLNCAGYGSSVCVTAPFGFAAAGLLLQAIALVGGQYRE"
     gene            8114..8731
                     /gene="ccmA"
                     /locus_tag="PKL42_03910"
     CDS             8114..8731
                     /gene="ccmA"
                     /locus_tag="PKL42_03910"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HNU66250.1"
                     /translation="MLVAQGLACLRGDRLLFKNVGFELNAGGLLYVLGENGSGKSSLL
                     RMLCGLLTPEEGAVFWNGKKIKEDAEQYLSNLTYLGHLNGLKDDLTALENLQTNARLA
                     GVEASEQAAMAALTAIGIARCAYLPARVLSQGQKRRVALAGLWLANTSFWILDEPFAA
                     LDVASVEVLAARLGEHIAEGGMAILTTHQDVTVNAKHTQTLRLSK"
     gene            8728..9396
                     /gene="ccmB"
                     /locus_tag="PKL42_03915"
     CDS             8728..9396
                     /gene="ccmB"
                     /locus_tag="PKL42_03915"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HNU66251.1"
                     /translation="MNGAWLTVLTRDLLQAYRRRSDIATTLFFFVIVSSLFPLGVGSD
                     SAILSTIAPGVLWVAALLAGMISLSRLFAADFADGALEQMLLSPQPLSLLVSAKILAH
                     WLVCGLPVVLLAPMIGLQYALPSEAIGILVVSLLLGTPALSLIGAIGAALTLGARGSG
                     LLVALLVLPLYIPVLIFGSLAVSASQHGMSPQAHLSLLAAISLLAVVLAPLATAAALR
                     ISVE"
     gene            9538..10299
                     /locus_tag="PKL42_03920"
     CDS             9538..10299
                     /locus_tag="PKL42_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HNU66252.1"
                     /translation="MRQSAYFSDNFYQHASPQTFYPTAGRMIPWFAWLAVVFVIYGLY
                     ISFAIAPTDFQQGEAYRIIFVHVPAAWMSMVIYLAMAFWAGVGLALNSKLSAMMAQAL
                     APTGAIFTLLALVTGSLWGKPMWGTYWVWDARLTSELILLFLYLGFMALQASIDEPRK
                     ADRAGALLALVGAVNIPIIYFSVKWWNTLHQGSSINMGAAPKMAATMFEGMILMALAF
                     WMYTIAVALIRVRCIILERERNAGWVKELSLKDHA"
     gene            10299..10466
                     /gene="ccmD"
                     /locus_tag="PKL42_03925"
     CDS             10299..10466
                     /gene="ccmD"
                     /locus_tag="PKL42_03925"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HNU66253.1"
                     /translation="MQWESWSAFFNMGGYAFYVWFSFGLTALCVVWEVIALRKRSSEA
                     LRLTRLLSQND"
     gene            10494..10952
                     /gene="ccmE"
                     /locus_tag="PKL42_03930"
     CDS             10494..10952
                     /gene="ccmE"
                     /locus_tag="PKL42_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNU66254.1"
                     /translation="MALKARHKRFILIGIGLGLIGLAAMLILNAFQSNMVFFYTPTQV
                     AQGEVPKNTGFRIGGLVEENSFKRGEDGLSVRFVITDTAKTVPVLYKGVLPDLFKEGK
                     GVVAQGKLGEDGMFTASEVLAKHDENYMPPEAAEALKQAQTQSNTVVMPK"
     gene            11169..13145
                     /locus_tag="PKL42_03935"
     CDS             11169..13145
                     /locus_tag="PKL42_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HNU66255.1"
                     /translation="MIPEIGHFSLILALLTAVLLGSIPLIGAARGNAVWIGLAKPLVN
                     VQFLLIAFAFICLAYAFATNDFSVAYVAKNSNSKLPLQFRIAAVWGGHEGSLLLWALI
                     LSMWTVAVGLFSKHIPDNMRARILGVMGLVSVGFLLFTLIASNPFERMIPAALDGSDL
                     NPLLQDPGMIFHPPMLYMGYVGFSVAFAFSIAALLGGQLDAAWARWSRPWTTLAWVFL
                     TIGIMLGSNWAYYELGWGGWWFWDAVENASFMPWLVGTALIHSLAVTEKRGSFKAWTV
                     LLAICAFSLSLLGTFLVRSGVITSVHAFATDPRRGVFILAFLVVVIGGSLALYAWRAP
                     KIGRGGQFDVVSRESFLLANNVLLAVAAMAVLLGTLYPLFVDALGYGKISVGPPYFDA
                     VFAPLMAPALFLMGVGPIARWKESSLPELATRLRWALAISLILAIALPFALGNFTAWI
                     GFGLFLAFWVFVTSFELIYKRLAKSTGSLIARIQANSRSWWGMVIAHLGIAVFTLGVT
                     IVKGYESEIAVKMAPGEVAHLAGYDFTFDGVKAVQGPNFEASVGQIHVSKNGKLRTVL
                     EPQKRIYTVSGMPMTEAGISPHFIHDLYASLGEPLGDGAWSVRIYHKPMVEWIWFGCL
                     MMGFGGILAIADKRYRLRNRSKNDTPSNTSGAAV"
     gene            13151..13678
                     /locus_tag="PKL42_03940"
     CDS             13151..13678
                     /locus_tag="PKL42_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HNU66256.1"
                     /translation="MLRALIPLAIFLALVGFLFKGLFLNPKEIPSPLVGKPAPQFDLP
                     RLDNPEATFSPKEMLGKVWMLNAWASWCPSCVEEHPVLVELAKTGIVPIVGLDYKDTR
                     PEALQWLEKGGNPYVAVAVDADGRVGIDYGVYGVPETYVIDKSGLIAYKQTGPLNARN
                     LNETILPLVEKLQAQ"
     gene            13675..14151
                     /locus_tag="PKL42_03945"
     CDS             13675..14151
                     /locus_tag="PKL42_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HNU66257.1"
                     /translation="MKISNFYRSAIIAFLMQWTLVHAQEAQPLQTNSAVEAQVQRLSE
                     ELRCLVCQNQTLADSHAGLAEDLKQEIRDMANKGMTDQAIIDYLVARYGDFVRYRPAL
                     NSTTLILWLGPFVLLLAGGIGLIFMLRRRQKISTDQPLSNDEARRVKELLDLDRKA"
BASE COUNT         3668 a         2696 c         3634 g         4155 t
ORIGIN      
        1 aaaataggcg gttagctcag ttggttagag cgcttggtcg acatccaaga ggtcaccagt
       61 tcgaatctgg tactgcctac cagtgtattt tcctcgttgt actgctgcat ttttagtctt
      121 ttagttcatt taaagtgtgc cgagcaaaaa aagatgctct ggaagccttt gtttataagg
      181 ctcgtagcca tgcgtaaaag gttggtgatt tttttgcaat aaaatgcaat tttatttgcc
      241 aattttgtaa tgtatgcgac acgcattttt ctgacgcttc agtctatttt gtttatcttg
      301 ataatttgct aaatagttga taaaaaatca agcggttgta gtaaaatcac ggctattgtg
      361 gagggcgctt aattgcgcct tatttgtttg taaaagctta aaaaagacct catgaaaaac
      421 attcgtaatt tttctatcat cgcgcatatt gaccatggta aatctacttt agctgaccgc
      481 attattcatt tgtgtggtgg tttggcagac cgtgaaatgg aggcgcaagt gcttgattca
      541 atggatattg aacgcgagcg cggtattacc attaaagcgc agacggcggc tttgcaatat
      601 aaagccgatg atggccaaat ctaccaatta aatctgattg atacgccggg tcatgtggat
      661 tttagttatg aggtttcgcg ctcactttca gcctgtgagg gtgcgttgtt ggtggtggat
      721 gcatcgcaag gtgttgaagc gcaaagtgtg gccaattgct ataccgcact ggatttaggc
      781 gtagaagtca cagcggtact caataaaatt gatttgccat cagccgaccc agagcgcgtt
      841 aagcaagaaa ttgaggatgt gattggcgtg gatgcgagtg acgccgtatt gtgttcagca
      901 aaaacgggct taggtgtgcg tgatgtgttg gaagccgtca ttaaaaaggt gccgccgcca
      961 caaggcaata ttgatgcgcc gctgaaggcc ttggtgattg atgcatggtt tgataattac
     1021 gtgggtgtgg tgatgttggt gcgtgtggtt gacggcgtgc tcaagcccaa agataaaatc
     1081 cgtttaatgg cgacgcatag tgactttttg tgcgagcaag tcggcgtgtt tacaccaaaa
     1141 tcattgcaaa aatctttgct gtcagccggc gaggtgggtt ttgtgattgc tggcattaaa
     1201 gagttatcaa gtgcaaaagt gggtgatacc gttactttgg cgaataagcc agccgcagcg
     1261 ccattggcag gttttaaaga agtaaagccg caagtgtttg cgggtttgta ccctgtggaa
     1321 tctaatcaat ttgaagcatt gagaactgcg cttgaaaagt tgagtctgaa tgatgcttcg
     1381 cttttgtttg aagctgaaaa ttcaacggcg ttgggctttg ggtttagatg cggcttttta
     1441 ggcttgttgc acatggaaat tgtgcaagag cgcctagagc gcgaatatga catggattta
     1501 attactaccg cgccgaccgt ggtttatgaa atattgctca aagatggttc agtggtgcaa
     1561 attgaaaatc catcccgctt acctgagccg tcccgtgtag aagaaacgcg cgaaccgatt
     1621 attgtggtga atttgctgat gccgcaagat tacgttgggc cggtgatgac gctatgtaac
     1681 aacaaacgcg gcatacaaaa aaacatgcaa tatatggggc gtcaggtgat gttgagctat
     1741 gaaataccgc tcaacgaagt ggttctcgat ttttttgata aattaaaatc tgtatcgcgt
     1801 ggctatgcgt ccatggatta tgagtttttg gaattccgtg ccgcagattt ggtgaagctt
     1861 gatattatgg tcaatggtga gcgtgtggac gcattgagca tgattgtaca taggtcaaac
     1921 agcgtgcatc gcggtcgaga agtggccgct aaaatgcgcg agttgattcc acggcaaatg
     1981 tttgatgtgg cgattcaggc atcgattggt gcaaatatta ttgcccgtga aacagtaaaa
     2041 gccatgcgca aaaatgtgat tgccaaatgt tatggcggtg acgtttcacg taaacgtaaa
     2101 ctgctagata aacaaaaaga aggtaaaaaa cgcatgaagc aagtgggtaa tgtagaaatt
     2161 ccacaagaag catttttagc gattttacgt gttgaagata aataatttag aaagtaagga
     2221 ttaaacacaa atgattgttt atttggttct agggttaatg cttttttgct taatcttgtt
     2281 gatacagcgc aagctcaccg taggcttatt tttgttttct gtggcaggtt tgttggggct
     2341 gttgcttacg gcaaccagta tcatcccgtt tgccttattc atgctgttga tgttggtgtt
     2401 aaccggggtc atttggctgg tggacagttt gttctttaag gcaaaacgag aaaatgccgg
     2461 tgaataccct gaagtggtag agtatgccag aagttttttc cccgtgatct tggtggtatt
     2521 ttttgtaagg tcttttatcg tggagccttt taaaattcca tcagggtcga tgatgcctac
     2581 gcttttggca ggtgatttta ttctggtgaa taaatttcac tatggtttgc gtgtaccagt
     2641 cttgaacaat acctttattg aggtgaatca tccaaaacgt ggcgacgtgt tcgtgtttca
     2701 ttatccacca gacccttcta ttgactatat taaacgagtg gtggggctgc cgggggataa
     2761 aattcggtat caaaacaaaa ggctcaccat taacggtcag ttgcttgaac tcaatgaggt
     2821 tggtcaatac aattatgtga gcgcagggtt aaatatggtc acagcaacac aatatcgaga
     2881 aaaattgggg gaggtgagcc atgaaattct caataatgac tgggttggaa attacgaaca
     2941 tgattctatc ggtgccaagt ttgccaacga tgaagagatt actgtgcctg ctggacatta
     3001 tttagcgatg ggtgataatc gcgacaatag ttcagatagt cgtgtctggg gatttgtacc
     3061 agagcataat ttagtgggta aggcattttt tatttggatg aatttcgacc aaggttctag
     3121 aatcggaaaa tcagtgcagt aatttcttta gtgtttaaat ggataacttc atcttcaggg
     3181 aggctttatg ttaaataaaa tcaatactca gaaacagttt ttacaatcgt taccagtaaa
     3241 aaaacaggca ggtgcaacgc tcctaggcat gatgtttgta ggaggtatga ttgtttttgt
     3301 ggcgctgatt gccatgaaga tgtttcctgc ctatcaggag tatttttcag ttaaaacgat
     3361 tattcgcgcg atgaagcaag aagtgggcaa tatgagcaaa aatgaaatcg ccgcttcttt
     3421 tgatcgccgt gctgatactg gctatgtcac tgtaattagg ggtaaagatt tagaaatcgg
     3481 taaaaatgcg agtggtgata caacggttac tgcaaggtat cgtgtggtga caccactctt
     3541 tggcaatgtc agtgtactta tggattttga gacatccacc gacagcaagc tttaatgcaa
     3601 gcacctgata taccccgcat gactctacag ttgttagcca aacgcctaaa acatcaattt
     3661 gaagatgaaa atctgttaag tcaggcgctc acgcaccgta gttatagcgg taaaaataac
     3721 gagcgattag agtttttagg cgatggcgca cttaatttta ttatcgccaa ccagctttat
     3781 cagcgctttc agaccctgga tgaaggcgat ttaagtcgtt tacgtgcaca acttgtgaaa
     3841 gaggccactc tttgtgaaat tgccctatca ctagggctag gtgatgcact caaactgggc
     3901 gaaggcgaac tcaaaagcgc tggctggcgc aggccttcaa ttttagcgga tgcgttagag
     3961 gctatcatcg gtgctaccta tcttgatggc ggctttgtgg cggcagaagc gctagtactt
     4021 gacctttatg ctgaaaagct cagcaccatt gacccaaaag tcattgataa agatcctaag
     4081 tctttactgc aagagttttt gcaaagtaaa aaaattgcgg tgccagaata tgccgttgtg
     4141 cagacgagtg gtgaagcgca tgcacaaata tttgtggtgg agtgttttat tcaaaagtta
     4201 gatattcgta ccgtaggcga aggcacgagc cgtcgtatcg ctgagcaaaa agctgctcag
     4261 ttagctatgc agcaagtaga acaactaagt ataagaaaac ctaagaaata aaacattaga
     4321 gatgagaaaa cgttaaaaat gactcaaacc acagttggat ttaagtgcgg taccgttgcc
     4381 attgtcggcc gtcccaacgt gggaaaatcg actttgctaa atcatatttt agggatgaaa
     4441 ttagccatta cctcacgtaa ggcgcaaact acacgacatc gcttacttgg tattcataca
     4501 acagatgaca cgcagttttt gtttgtagat acccctggct ttcagcaaaa acatcttaac
     4561 accttgaata aaggtttaaa taaaacagtg acacaagtgt tgaccgaagt cgatgtcgtg
     4621 ctgtttgtga ttgaacccat gaaattgggt gaagcggatg aaaaggtgtt agcactgcta
     4681 cctaaagata aacccgtgat cttggtggtg aataaagcgg atttgatggg cgacaaaaat
     4741 aatttgctac cattgattca agactttgat ttgctgcatc catttgtcgg tattgtgccg
     4801 gtaagtgcaa aaaaatcatt gcatttagaa gagttgcttg aaactactcg caaatacttg
     4861 cctgaacagc cggccgtgta tggtgaagat gagctcaccg ataaaaacga acggttttta
     4921 gccgcggagc tgattcgtga aaagattttt agattgctag gcgatgaagt accttatgca
     4981 gtagcagttg aaattgaaaa gtttgaagtg gtaggtaaat tgcgccgaat ttttgctgcg
     5041 attattgtag ataaagacag tcaaaaaccc atgttgattg gaaaaaatgg cgaaaaaatg
     5101 aagcaaatat ccaccgaagc ccgccaagat atggaaaagt tttttggtgg taaggtttac
     5161 ttggaaactt gggtaaaagt acaaggcggc tgggctgata atgcacgtgc gctgaaaagc
     5221 ttgggttact aattcgcaat aagtgaaagc atggcgttag caagcaacct tcgtcaagat
     5281 aatcaatccg tgtatgtgtt gcacagctat ccgtttaaag aaaccagttt agttgtagaa
     5341 ctctttagcc agcagtttgg ccgtgttgca gcagttgcaa aaggtgcgcg ccggccaagg
     5401 tctgcaatgc gcggcatgtt gcaatcattt cagcttttat ctggcacatg gtcaggtaag
     5461 aatgagttaa aaacactgca tagtttggat tgggcgcatg gtctagttaa tttacaaggt
     5521 gaagccctga tgtgcggttt ctacatgaat gagcttttgt tgcgtttact cccccgtgaa
     5581 gatgcgcacg aagcgctgtt tgattattat caaacaacac tcagtacgtt aagtaaatca
     5641 actgatttgg ctacacatct acgccgcttt gagcttaaac tattgcaaga gttgggctat
     5701 gccgtgccgc taattgcaga tgaacatggg gaagtgattt cagccagtaa gcaatatcgc
     5761 tatgaagctg aatttggtgc gagagcgcca catgaaaatc ataatggcgt acagttaagt
     5821 ggaagtacgt tattggatat ggcgaaagat aattaccaga atagcctgac gcaagcgcaa
     5881 agtaagcagc tcatgcgcta tttattagcc cattacttgg gtgataaacc cttacacacg
     5941 cggcaacttt taattgattt acagggtttt taagatgatt aaattaggtg tcaatataga
     6001 ccacgtagcc actatacgtc aggcgcgcgg aacgaagtat cccagtgtag ttcaggccgc
     6061 gcttcgcgct gagcaatctg gcgcggatag cattacccta catttacgtg aagaccgccg
     6121 ccacatgcaa gatgcagaca tttttgcctt gcggcccttg ctgcaaacta aaatgaatct
     6181 cgaatgcgcg gtgacggacg agatgattga aattgcccta aaggttgcac cgcaagatgt
     6241 ttgcttagta ccagagcgcc gcgaggagcg tacgacagaa ggtggcttgg atgtcatcgg
     6301 tcactttgat aaaataaaaa aggccgtgca aagattaggt gatgcaggta ttcgtacatc
     6361 gctatttatt gcgcccgatt tggcacaaat tgatgcagcg gtaaaaacag gcgcaccagt
     6421 cgttgagctg catacaggta cttttgcaga tgcgcacact gatgctctgc aggatgtaga
     6481 acgtaagcgt attgaaattg cactacatca cgctaaagat gcgcatttgg tggtgaatgc
     6541 tgggcatggt ttgcattatc acaacgtgca ccacattgct aagatgtacg gatttgaaga
     6601 actgaatatc ggccatgcca ttattgcgca tgcgttattt gtgggttggg ataatgcggt
     6661 gcgcgagatg aaagcgttaa ttaaagagtt ttctcctaat taagccatga tttacggcat
     6721 tggcacggat attgtagaag tggcgcgaat agaagcctcg attaaccaat ttggcgatga
     6781 cttagcgaaa cgcatcttgg ctgaaagtga gtttaaaagt tatatggatt caaatattaa
     6841 accacgtttt ttagccaaac gttttgctgc aaaagaagct ttttctaaag cgttaggcac
     6901 aggtttgcgt gcaccagcca ccatgcaaaa tattgcagtt tcgcacgatg atttaggcaa
     6961 gccaattttg gtgttggcga atgaattgca agcttttttg caagctaaag acatcacaaa
     7021 aatgcacatc agtatcagtg atgaaaaaaa tttagccgct gcgtttgtgg tgttagaaag
     7081 cacaaattga actacaaatt ttgccttaaa tatttatttt aaaagcagca acaaacgccc
     7141 gagagaaaac aatgcctctg tgagccttac tttaggcggc ttgcagaggt gttgaaaagg
     7201 tttgtattaa aattacgtta tttttaagac ttttattatt caaatatgac agctttaagc
     7261 cttgatttta gccgccgttt tggcggcgtt gcgcgccttt atggcgcaga cggcttgacc
     7321 aacttgcaag ctgcacatat ttgcgttatt ggcatcggcg gcgtgggttc atgggcagca
     7381 gaagccgtgg cgcgcaatgc ggttggtaag attactttga ttgatttaga caatattgcg
     7441 gaatctaatg tgaatcgcca attacacgcg ttggatggcg cgtttggcaa agcaaaagtt
     7501 actgcgatgg ctgagcgcat caagcagatt aacccgcatg caacggttat tcaaattgaa
     7561 gatttcgtca gccctgagaa tatagcgaca ctattgaatc agccttatag cggggttatc
     7621 gattgcattg atgatgccaa agccaaagca gccattgcgg atttttgcaa aaaaaatgcc
     7681 attcctttgg tgatgacagg ggcggcaggt gggcgattaa acgccacgca tatcaaacaa
     7741 gccgatttaa gtgtggtgag ccacgataaa ttgctggcaa aagtgcgcaa cttgctacgc
     7801 cgcgagtatg gctttgccaa agcgatttca ggtaaaacct caaaatttgg cgtgttgtgt
     7861 gtgtattccg atgaatccgt gattaaacct gaaggtgttt gtgatacaga ggacgttggc
     7921 atcaccggtt taaattgtgc gggctatggt tcaagtgtat gcgttaccgc gccatttggc
     7981 tttgcagcag ctggattact acttcaagcg attgccttag ttggtggtca atatcgagaa
     8041 tgatacaatt acccttcatg atttcgtcct tgcttaattt aaatttatta tcctgcgcag
     8101 ggttctttaa accatgttag ttgcgcaggg tttagcctgt ttacgcgggg atcgcttgct
     8161 gttcaaaaat gttgggtttg aactaaatgc gggtggtttg ttgtatgtat taggtgaaaa
     8221 cggcagtgga aaaagtagtt tattgcgcat gctatgcggt ttgctgactc ccgaagaagg
     8281 cgcagtgttt tggaatggca agaaaattaa agaagatgct gagcagtatt tatccaatct
     8341 cacctacctt ggccatttaa atggcttaaa ggacgacctc accgcgctgg aaaatttgca
     8401 gaccaatgca cggttagcag gtgttgaagc aagtgagcaa gccgcaatgg cagcacttac
     8461 ggcgataggc attgcgcgtt gcgcctattt gcccgcacgg gtgctttcac aaggccaaaa
     8521 gcgtcgcgtt gcgttagccg gtttatggct ggctaacact tcattttgga ttttagatga
     8581 gccatttgcc gcattggatg tggcatctgt tgaagtgttg gccgcaaggt taggcgagca
     8641 tattgccgaa ggtggcatgg ccatactcac cacccatcaa gatgttactg ttaacgcaaa
     8701 gcatacgcaa acgttgaggc taagcaaatg aatggcgcgt ggttgactgt tttaacgcgg
     8761 gacttactgc aggcgtaccg caggcggtca gacattgcga cgactttgtt tttctttgtc
     8821 attgtgtcta gcttgtttcc gttgggtgtt ggttcggatt cagccatttt aagtacgatt
     8881 gcacctggag tgctttgggt agcggccttg ctagcaggca tgatttcact atcgaggctg
     8941 tttgcggcag attttgccga cggcgcttta gagcagatgt tattatcacc tcagccttta
     9001 tcattgttgg tatcggccaa aattttggcg cattggctgg tgtgcggttt gccagtggtg
     9061 ttgcttgcgc cgatgattgg tttgcaatat gcattgccaa gcgaagcgat tggcattttg
     9121 gtggtttcac tcttgctggg tacacccgca cttagcctga taggcgctat tggcgccgcg
     9181 ctgactttgg gcgcacgcgg cagtggttta ttggttgcat tgttggtgtt gccgctgtat
     9241 attcctgtgc tgatatttgg ctcgctggca gtgtctgcca gccagcatgg gatgagtcca
     9301 caagcgcatt tatctttgtt ggcagcaatt tcgctcttgg ctgtggtgct tgcgccactg
     9361 gcaacggctg cggccttacg catttctgta gagtaataaa aacctatgag tttaaagaaa
     9421 attatatttg ccttaggctt aatcattgcg gtaagtgccg ctgcgctgat tttttttacg
     9481 cacggtctta aatacatcag tgtgaacgtt gccttgttag ccttaggggc ggtgctgatg
     9541 cgccaatctg cctacttttc agataatttt tatcaacatg cctcaccgca aaccttttac
     9601 cctactgctg ggcgaatgat tccgtggttt gcttggttgg cggtagtatt tgttatttat
     9661 ggcctctata tcagctttgc gattgccccg accgatttcc agcaaggcga agcctaccgc
     9721 attatttttg tgcatgtgcc agcggcctgg atgtcgatgg tgatttactt agccatggct
     9781 ttttgggcgg gtgtaggatt ggcgctaaat tcaaaacttt cagccatgat ggcgcaggcg
     9841 ctcgcgccca cgggtgcaat attcaccttg ctggcgctgg taacggggtc actctggggc
     9901 aagccgatgt ggggcactta ttgggtgtgg gatgcacgtt taacttctga gctgatttta
     9961 ctatttctat atttaggctt tatggcattg caagcttcta ttgatgaacc gcgcaaagct
    10021 gaccgtgcgg gtgcgttatt agcgctcgtc ggtgcagtga atatcccgat tatttatttt
    10081 tcagttaagt ggtggaatac tttgcatcaa ggatccagca ttaacatggg cgccgcaccc
    10141 aaaatggccg ctacaatgtt tgaaggcatg attttaatgg cgcttgcgtt ttggatgtac
    10201 accattgctg tggcactcat tcgtgtgcgt tgcattattt tagaacgaga gcgtaatgct
    10261 ggctgggtta aagaattgtc actaaaggat cacgcataat gcaatgggaa agttggtctg
    10321 ctttttttaa catgggcggt tacgcatttt atgtgtggtt ttcttttgga ttaacggcac
    10381 tttgtgtggt ttgggaagtc atagccttgc gtaaacggag cagtgaagcc ttgcgtttaa
    10441 cgcgattact ttctcagaat gattaacaag catacatata ggtgtaaaaa ataatggcat
    10501 taaaagcacg acataaacgg ttcatactta tcggtattgg cttaggctta attggcctag
    10561 ctgcaatgct gattttgaat gcgtttcaaa gtaatatggt gtttttttat acgccaacac
    10621 aagtggcaca aggtgaggtg ccaaaaaata ccggttttcg aataggcggg ttggtagaag
    10681 aaaacagctt taaacgtggc gaagacggtt tgagcgtgcg ctttgttatt acagacacgg
    10741 ccaaaacggt gccggtactt tataaaggcg tgttgcctga cttgtttaaa gaaggcaagg
    10801 gtgttgtcgc gcagggtaaa cttggtgaag acggcatgtt taccgccagc gaagtgttgg
    10861 ccaagcatga tgaaaactat atgccgccag aggctgcgga agcattaaag caagctcaaa
    10921 cgcaaagcaa taccgtggtg atgccaaagt aattttggcg tttaaataac actcaaaaat
    10981 tgtcaaaaaa agcacaaccc tttttagctg ctctgtaagc cttttaaata aaggctcaca
    11041 gagcagtaaa attttctcga cgtgttttgc ttcagttttt ttgtttttta aaacatataa
    11101 agtagtactt tttggttgcg aatgagcaat cgcaaagcaa ccgacaaaat tgtgataagg
    11161 atgtagatgt gattcccgaa attggtcatt tttctttaat actcgcgtta ctaacagcgg
    11221 tgttattagg cagtattcct ttaattggtg ctgcgcgcgg taacgcagtg tggatagggc
    11281 tagccaaacc tttggtgaac gtacaatttt tgttgattgc ctttgcgttt atttgtttgg
    11341 cctacgcgtt tgccaccaat gatttttctg tcgcctatgt ggcgaagaat tcaaactcaa
    11401 aattacccct gcaatttcgt attgctgccg tttggggcgg ccatgaaggc tcattgttgt
    11461 tgtgggcttt aattttaagt atgtggactg tggccgttgg cttgttttca aaacatattc
    11521 cggacaatat gcgggcgcgt attttgggcg tgatgggctt agtgagcgtt ggctttttgt
    11581 tatttacgct aatcgcatca aatccatttg agcggatgat accagccgca ttggatggca
    11641 gtgacctcaa cccgttattg caagacccgg gcatgatttt tcacccacca atgttgtata
    11701 tgggttatgt tggtttttca gtggcatttg ccttttcaat tgcggcattg cttggcggcc
    11761 agttagatgc cgcgtgggcg cggtggtctc gcccttggac aacgttggct tgggtttttc
    11821 taaccatcgg cattatgctc ggcagtaact gggcgtatta tgagttaggt tggggcggct
    11881 ggtggttttg ggatgccgtt gaaaatgctt catttatgcc gtggttagtt ggcacagcgc
    11941 tcatacactc gttagctgtg acagaaaaac gcggcagctt taaagcgtgg acggtcttgc
    12001 tggcgatttg tgctttttct ctcagcttgt tgggtacttt tttggtgcgc tccggcgtga
    12061 tcacttccgt gcatgctttt gcaactgacc cgcgacgcgg cgtgtttatt ctggcgtttt
    12121 tagtggttgt cattggcggt tcgttagcgc tatatgcttg gcgtgcccca aagattggtc
    12181 gtggtggcca gtttgatgta gtttcgcgcg agagctttct tttggcaaat aatgtgttgc
    12241 tggcggttgc ggccatggcg gtgttacttg gcacgttata cccgttattt gttgatgcgc
    12301 taggctacgg caaaatttca gttggcccgc cgtattttga tgccgttttt gcgccgctta
    12361 tggcgccagc gttgttttta atgggcgttg ggccaatagc ccgctggaaa gaatcttcat
    12421 tgccagaatt ggcgacccgt ttacgctggg ccttagctat tagccttata ttggccattg
    12481 ctttaccttt tgcgctgggt aactttactg cttggattgg ctttggtttg ttcttggctt
    12541 tttgggtgtt tgtaacgagt tttgaattaa tctataagcg cttagccaag tctaccgggt
    12601 cattgattgc ccgtattcag gccaattcaa ggtcatggtg gggcatggtg attgcgcatc
    12661 ttggcattgc ggtatttacg ctgggggtca ccatcgtaaa aggctatgag tctgaaattg
    12721 ccgtaaaaat ggcgccgggt gaagtggcgc atttagcggg ttacgacttt acttttgatg
    12781 gtgtaaaagc cgtgcaagga ccaaactttg aggcaagcgt tgggcaaatt catgtaagta
    12841 aaaacgggaa attgcgtacc gtgcttgagc cgcaaaaacg catatacaca gtctctggca
    12901 tgccgatgac cgaggctggt atcagcccgc attttattca tgatttgtat gcatcattag
    12961 gcgagccttt gggcgatggc gcttggagtg tgcggattta ccataaaccg atggtagagt
    13021 ggatttggtt tggctgcctg atgatgggtt ttggcggcat attagcgatt gccgataaac
    13081 ggtatcgact cagaaatcgc tccaaaaatg acacgccatc taacacaagc ggggctgctg
    13141 tatgaaatcc atattgcgcg ctttaattcc gttagctata tttttggcat tagttggttt
    13201 tctatttaaa ggcttatttc ttaatcctaa agagattcca tcgccactgg tgggcaagcc
    13261 tgcgccacag tttgatttac cacgtttaga taatccagaa gctacgtttt cacccaaaga
    13321 aatgctgggc aaagtatgga tgttaaacgc ttgggcatct tggtgcccat cgtgtgtgga
    13381 agagcatcct gtgttggtgg aattagcaaa aactggcatt gtgcctattg tgggcttgga
    13441 ttataaagat acacgcccag aggctttgca atggttagaa aaaggcggta atccttatgt
    13501 ggccgtggcc gttgatgccg atggacgcgt cggtattgat tatggggtgt atggtgttcc
    13561 tgagacctat gtaattgata aatcagggct gattgcctat aagcaaactg gaccgctcaa
    13621 cgcaagaaat ctgaatgaga caattttgcc tttagttgaa aagttacaag cacaatgaaa
    13681 atttcaaatt tttatcgctc agcaatcatc gcatttttaa tgcaatggac gcttgttcat
    13741 gcacaagagg cgcaaccatt gcaaaccaac agtgcggttg aagcgcaagt gcagcgtctt
    13801 tcagaagagt tgcgctgttt ggtttgccaa aaccaaacac tggctgattc acacgcgggc
    13861 cttgcagaag acctaaaaca agaaattcgc gacatggcca acaaaggcat gacagatcaa
    13921 gcgattattg attatctagt ggcgcgttat ggcgactttg tgcggtatcg cccagccctt
    13981 aattctacaa cgctgattct ttggcttggg ccatttgtgc ttttattggc gggcgggatt
    14041 gggcttattt ttatgttacg taggcgtcaa aaaataagca ctgatcaacc acttagcaat
    14101 gacgaagcgc gccgtgtaaa agaattatta gatttagata ggaaagcata atg
//
LOCUS       DAOQSI010000037        14138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_3362_length_14138_cov_5.459703, whole genome shotgun sequence.
ACCESSION   DAOQSI010000037 DAOQSI010000000
VERSION     DAOQSI010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 14138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14138
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3362_length_14138_cov_5.459703"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..1064
                     /locus_tag="PKL42_03950"
     CDS             69..1064
                     /locus_tag="PKL42_03950"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNU66258.1"
                     /translation="MAKRQYFKKLAAHQDLLVLLAVLMLLLLALLKPSVPVKHDIHSY
                     FFIVDITQSMNAVDMQVNGKPASRLDHTKQLLRETISSLPCDTKVGLGMFSGVNVVTL
                     YLPIEVCSNFSAIQDTLDHIDWRNAWTADSRVRESMLSTSRVVSNFPEPAQVVFFSDG
                     EEAPKLHAFNTRDLSTLQNADGWLLVGIGSEKGAAIPKFTEKNQLLGYWSHESMQLAP
                     GAAPIAAAGLLNRKKDIAEDINDRYLSKLSEATLQAMAKEIGATYVRGDTYMTLQSAM
                     KKQKPARREWAPWSLSSALAGLAGLFLLLAYVPFSRFVKKDIASKQPPAFISAQL"
     gene            complement(1045..1467)
                     /locus_tag="PKL42_03955"
     CDS             complement(1045..1467)
                     /locus_tag="PKL42_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66259.1"
                     /translation="MLCMLIAAALFFASMLFVVPIAWRYWSAFRWVLLAFIVLVTIYV
                     IAKNALLSLPWSYVGLSIDSKNQLQLLRKNGTRLDVLVRPSTTVTAFLTVLNGQVKSP
                     TLLEKIFTLHVLILPDTLNAEDYRQLRVWLRWAKVVQK"
     gene            1546..2583
                     /locus_tag="PKL42_03960"
     CDS             1546..2583
                     /locus_tag="PKL42_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HNU66260.1"
                     /translation="MANNDWQEFLFIKGAVFSNGTISSFGNTEKELAYAHTDNIICDL
                     SHLDLLEISGDDALAFLQGQVTNDVKLLSENNAHYTGYCSPKGRLLALFFAFKFNNQL
                     HLQLNHKLAEPIAKRLKMYVMRSKVHINNISDNTVSVGLSGNDVPQLLSSFFTQIPQH
                     AYETSHTDGATLIRLSGALPRFEIVGQTEMIKTIWQTLKTSCKPVGKPCWEWLEIQAG
                     IPDIVVTTQEEFVPQMVNLDCLGAINFKKGCYTRQEIVARTHYLGKVKRRTQLAHIAT
                     STPPQAGNDVTNQNNDAVGKIVRSAPAPSGGYDVLAEVRLEALAEGTVFWNNLALQIQ
                     PLPYSFDAATH"
     gene            2910..3524
                     /locus_tag="PKL42_03965"
     CDS             2910..3524
                     /locus_tag="PKL42_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNU66261.1"
                     /translation="MHDLLSDEALMQQFCAGKQQAFALLYARHEKPLYRYVRRVLGQS
                     LAIHADDIFQDTWMKLVDAKSTWQAQENASFKTWLFTLAHHRAVDILRKNGREISVDD
                     GWLDDNSESPWQLWPASTAEQPEQQAFWRKAGQQLLDCLEGLPALQRAAFLLHHEDGL
                     SLDEMTQVLSAEFETVKSRLRYALGKLRACMGAYLHPMIEKDQS"
     gene            3524..4576
                     /locus_tag="PKL42_03970"
     CDS             3524..4576
                     /locus_tag="PKL42_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66262.1"
                     /translation="MHLQDEHLKTALQHAPDRDVQPKAHVRDAVLRYAKKATVPQPSK
                     LQRLRYWLFYGKLSQAQWAGIGSVATVLLVVFMAWQQHPEEHVWITAETNNQNAAHTE
                     MAASAPAPVSQDAEITPATAPLAQEQLNDFAQEKPIATQNEQDKYDSETPTKRLKGNV
                     IEEKLTGKKSVSKDAETVKPLEKAAHEASQTSVSESNAASKELPAELSAQHQPSPVVA
                     ATAPQVNLPESKISLGAADKLESRAIANKTRETLANSQSALVEQLITQGGQKVAHQDI
                     KNEVLRILNVVVHQAEVRNQDACTLNQTPSTLVDAATGLRIENIDICADSEQLQKEVE
                     SYNKTMKSGYLQYQGE"
     gene            complement(4746..5057)
                     /gene="ftsB"
                     /locus_tag="PKL42_03975"
     CDS             complement(4746..5057)
                     /gene="ftsB"
                     /locus_tag="PKL42_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HNU66263.1"
                     /translation="MKALTLIFVVLIALLQYPLWLGKGSWLRVWNLNTQITAQQEKND
                     ALKMRNEALNAEVRDLKSGRAAIEERARSELGMIKQDEVFYQVLQNEVPAAPAPQNSA
                     P"
     gene            complement(5172..6458)
                     /gene="eno"
                     /locus_tag="PKL42_03980"
     CDS             complement(5172..6458)
                     /gene="eno"
                     /locus_tag="PKL42_03980"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNU66264.1"
                     /translation="MSAIVDIIAREILDSRGNPTVEVDVLLESGIIGRAAVPSGASTG
                     AKEAMELRDGDKSRYLGKGVLQAVENVNTEITEAIIGLDAEEQSFIDKTLIELDGTEN
                     KDRLGANAILGVSMACARAAAEESGLPLYRYLGGSGAMQLPTPMMNIINGGAHADNSV
                     DMQEFMIIPAGLPSFREAIRCGAEIFHQLKKTLHKKGLATTVGDEGGFAPNLPSNEAA
                     IQLIMESISDAGYEPGRDVYLGLDCASTEFYKDGKYHLESEGLALSSAQFTDYLATWC
                     DKYPIISIEDGMGEFDWEGWEILTKRLGKTTQLVGDDLFVTNAKILREGIQRGVANSV
                     LIKVNQIGTLTETFQTIEMAKRAGYTAVVSHRSGETEDTTIADISVATNALQIKTGSL
                     CRSERIAKYNQLLRIEEELGDASSYAGKGAFYQLFK"
     gene            complement(6507..6845)
                     /locus_tag="PKL42_03985"
     CDS             complement(6507..6845)
                     /locus_tag="PKL42_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66265.1"
                     /translation="MKKLLTILLLTSLTGCGDVIEKASDIIDILNKPSKKQIVQHLGS
                     ANCLKEYYDYWDYSSNKAFATSSDESCGWSGSHHETIEAAKKEAVEYCEQNRKGGTPC
                     KVVDVNGRWL"
     gene            complement(6854..7693)
                     /gene="kdsA"
                     /locus_tag="PKL42_03990"
     CDS             complement(6854..7693)
                     /gene="kdsA"
                     /locus_tag="PKL42_03990"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNU66266.1"
                     /translation="MKLCHFDVGLEHPLFLIAGPCVIESKQMAIDTAGQLKEITSALG
                     IPFIYKSSYDKANRSSNKTFRGFGMEEGLRILDDVRAQIGVPILTDVHTEEQVPHVAA
                     VVDVLQTPAFLCRQTDFITACAKSGKPVNIKKGQFMAPGDMKNVVQKAREANGGADNI
                     MVCERGVSFGYNTLVSDMRGLAIMRETHCPVVFDATHSVQQPGGQGDKSGGQREHVPV
                     LARAAVASGIAGIFMETHPDPAKALSDGPNAWPLGKMHDLLAVLIDLDRLVKKAGFAE
                     MTL"
     gene            complement(7704..9341)
                     /locus_tag="PKL42_03995"
     CDS             complement(7704..9341)
                     /locus_tag="PKL42_03995"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNU66267.1"
                     /translation="MTKYVFVTGGVVSSLGKGIAAASLGAILESRGIKVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFISQRMARRNSFTTGQIYETVIKKE
                     RRGEYLGKTVQVIPHITDEIKNHIKRGAAGADVAIVEVGGTVGDIESLPFLEAIRQMG
                     FEEGRNNACYVHLTLLPWIPTAGELKTKPTQHSVKELRQIGIQPDILLCRAEREIPED
                     EKRKIALFTNVAYEAVISAIDSDSIYKIPGLLHDQMMDEIVCHKLNILAKAADLTAWK
                     NIVTRLENPKNSVNIAFVGKYVDLTESYKSLTEALIHAGIHTESKVKINYIDSEELEK
                     SGTGSLKNMDAILIPGGFGVRGTEGKILAIQYARENKVPYLGICLGMQLAVIEFARNV
                     AGLTGANSTEFNASAPHKLIGLIDEWQDATGKIEKRDENSDLGGTMRLGAQACPIVQG
                     TMAASIYGAQVNERHRHRYEVNNHYVEQLKAAGLVVSARTPREDLCEIIELPKATHPW
                     FMACQFHPEFTSNPRSGHPLFIAYVKAALANQKAHSL"
     gene            complement(9483..10301)
                     /locus_tag="PKL42_04000"
     CDS             complement(9483..10301)
                     /locus_tag="PKL42_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNU66268.1"
                     /translation="MKLNTIAGFVLLSLAPALSHAGAVNWTSQKYNLNAYLDIDEPEQ
                     PVSLPQLNMTAFSLAGLPNISTASLTSANGVASAALGIKNLSNSMYDIVELSLNAFSS
                     YKGGTSAGSQYSYVVPSVSVENQFVADSNILTVTYDFSSLINLATPTVGQLSYNAMFI
                     GLSIVEDAFGATEQELKFVDNNFEAGTTVDKLFSGSGTAFFNVTPGKTYNVFADISPD
                     LFTSGTVSGNSSKSVLTLGFSNVAVAAVPEPETYAMLLAGLVLVGFSARRQTQA"
     gene            complement(10447..11091)
                     /locus_tag="PKL42_04005"
     CDS             complement(10447..11091)
                     /locus_tag="PKL42_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNU66269.1"
                     /translation="MQHLFEQLLRTSQKLTALGLNVGTAGNCSVRLNAATFLITPSGV
                     ATEAMSANSMVRMQFDGTVEPNKTPSSEWRFHRDILQNRPEINAVVHTHSMFATTLAC
                     LHIEIPAFHYMIAVAGGDNIRCAPYALFGSQALSDAALTALFERKACLLANHGMIALG
                     RDLDDALAVALEVENLCAQYWRTLQIGKPHILSTQDMQDVFQQFKGYGNWGENK"
     gene            complement(11252..12349)
                     /gene="mtnA"
                     /locus_tag="PKL42_04010"
     CDS             complement(11252..12349)
                     /gene="mtnA"
                     /locus_tag="PKL42_04010"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNU66270.1"
                     /translation="MKIEGKNYRTIWPSADTSADKPIVNIIDQTKLPHVFEIAEISTL
                     PQMLHAIKTMQVRGAPLIGAAAAYGMALAALHHDDLQEAAQLLIHSRPTALNLRWAVE
                     RMRNVLLPLSPESRKSAAWLEAARICDEDVQMNHQIGQHGLNVIQQIAASRKANQAVV
                     NILTHCNAGWLATVDGGTALAPIYAAHDAGLRVHVWVGETRPRNQGASLTAWELGQHG
                     VRHTVVADNAIGHLMQIGAVDMVIVGADRVTALGDVCNKIGTYLRALAAKDNHIPFYA
                     AVPSPTIDWSISDANLIEIEHRSSEEVSHMQGLSEAGHLQSVCILPAGSPAFNPAFDI
                     TPAQLVTGIMTEHGVYPPAELEPALHPKISL"
     gene            complement(12602..13384)
                     /locus_tag="PKL42_04015"
     CDS             complement(12602..13384)
                     /locus_tag="PKL42_04015"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNU66271.1"
                     /translation="MLIDTHCHLDAAEFNADRDAVALSALQAGVEKIVIPAVERANFD
                     AVIAVCQRHPHCAFALGVHPMYVDHATEDDLATLQTYVAKHQPVAIGEIGLDYFVKNA
                     KEHPAHIEKQLYFFTEQLKIANQFHLPVILHVRNAIDDVLKYLRKAKVNGGIAHAFNG
                     SFQQAEQLIALGFKLGFGGAMTYSRALHIQALAKQLPLEAIVLETDSPDIPPEWLGKQ
                     ARNTPAQTVKVAQFLADLRQVNVSQVLDITSANALQILPKLA"
     gene            complement(13398..>14138)
                     /gene="ribD"
                     /locus_tag="PKL42_04020"
     CDS             complement(13398..>14138)
                     /gene="ribD"
                     /locus_tag="PKL42_04020"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNU66272.1"
                     /translation="GIEVAVGLMESVAFNLNSGFIARMTRGLPYVRCKIAASLDGRTA
                     LNNHQSQWITGEAARADVQHWRAQSCAIITGIGTVLADNPCMNVRLANVSRQPLRVIV
                     DSQLQTPLDCNLLSTSHPQAAPVLIAYANDANQQQQLLMAKGVRLLQLPNQNGNVDLT
                     ALLATLAQQGVNEVLLEAGQGLNGAFLQAGLIDELILYYAPKLMGTDAKGMFAVPELT
                     NMQQVTHLQIIDVRQFGQDIRLRAKPKK"
BASE COUNT         3919 a         3400 c         2999 g         3820 t
ORIGIN      
        1 caaacgtaca cacttgagcc acaatgaagc cagcattgca catgacaatc actttactta
       61 actaggcgat ggcaaaacgt caatatttta agaagttagc cgcacatcaa gatttattgg
      121 tgttgctggc ggtattgatg cttttgttac ttgctttgtt aaagccaagt gtgcctgtta
      181 agcatgacat tcatagttat tttttcattg tcgatattac gcaaagcatg aatgcggtgg
      241 acatgcaggt gaatggcaaa cctgccagcc ggttagacca taccaaacaa ttattacgtg
      301 aaaccatctc atccctgccc tgcgacacca aagtgggctt gggcatgttt tcaggggtta
      361 atgtcgtcac cctgtattta ccaatagaag tctgcagtaa tttttctgcc atacaagata
      421 cactggacca cattgactgg cgaaacgctt ggacagccga cagccgcgtg cgtgaaagca
      481 tgctgtccac ctcgcgcgtg gtgagtaatt tccctgaacc ggcgcaggta gtttttttta
      541 gtgacggtga agaagcacct aaattacatg cttttaatac gcgcgattta tccaccttgc
      601 aaaatgctga tggctggctt ttggtaggca ttggcagtga aaaaggtgcg gccattccaa
      661 aatttactga aaaaaatcag ctactaggct attggtctca tgaaagtatg caattagcgc
      721 caggcgccgc accgattgcg gcggcggggt tgctgaatcg caaaaaagac atcgctgaag
      781 acatcaacga ccgctattta tccaagcttt ctgaagccac cttgcaagcc atggcgaaag
      841 aaatcggtgc aacgtatgta cgcggtgata catacatgac actacaatcc gccatgaaaa
      901 aacaaaagcc cgcacggcgt gaatgggcgc cttggtcact ttcatcagca ttggcggggc
      961 ttgctggctt gttcctgttg ttggcttatg tgccgtttag ccgttttgta aaaaaagaca
     1021 tagcaagcaa acagccacct gcttttattt ctgcacaact ttagcccaac gcaaccaaac
     1081 tctaagctgg cgataatctt cagcattaag cgtatctggc aatatcaaca catgcagtgt
     1141 aaaaattttc tctagcagtg taggagattt tacctgacca tttagcaccg tcaaaaaagc
     1201 agtcactgtc gtactaggcc gtaccaaaac gtccaagcgc gtaccattct tacgcagtaa
     1261 ctgtagttgg tttttgctgt caatactcag ccccacataa gaccaaggca ggcttaataa
     1321 cgcatttttt gcaataacat aaatcgtcac cagcacaata aaagccaata atacccaccg
     1381 aaacgctgac caatatcgcc atgcgattgg tactacaaat aacatgctag caaaaaatag
     1441 cgcggctgca atcagcatgc agagcatgat gcgcgatgga tggaatgtca ggttaagtgg
     1501 cttcacgtta taatgaacaa tatcaatttt attttagagt atcacatggc aaacaacgat
     1561 tggcaggagt ttttatttat taaaggtgct gtgtttagta atggcacgat tagttctttt
     1621 ggcaacactg aaaaagagct cgcctatgca cacactgaca atatcatttg cgatttatcc
     1681 catcttgatt tgcttgaaat cagtggtgac gatgcgctgg cttttttaca aggtcaggtc
     1741 acaaatgatg ttaaattgtt gagcgagaat aacgcacatt acacgggcta ctgcagccct
     1801 aaagggcgtt tgctggcatt attttttgcc tttaaattta acaatcaact gcacttgcaa
     1861 cttaaccaca aattggccga acccatcgct aaacgcctca aaatgtatgt gatgcgctca
     1921 aaagtacaca tcaataatat ttctgacaac actgttagcg ttggcctaag cggtaacgat
     1981 gtgccacaat tgttaagcag ttttttcacg cagattccac agcacgctta tgaaactagc
     2041 cacacagatg gcgcaacgct tattcgtctt tcaggcgcgc taccccgttt tgagattgtt
     2101 ggccaaactg aaatgatcaa aacaatatgg caaacgctca aaaccagctg caaacccgta
     2161 ggtaaaccat gctgggaatg gttagaaatt caggcgggca ttccagatat tgtggtgact
     2221 acgcaagaag aatttgtgcc gcaaatggtc aatttagatt gtttaggcgc aatcaacttt
     2281 aaaaaaggtt gctatacccg ccaagaaatt gtcgcccgca ctcactattt aggcaaagtg
     2341 aaacgccgca cgcaacttgc gcacattgcc accagcacgc caccgcaggc tggcaatgat
     2401 gttacaaacc aaaataatga cgcggtcggt aaaattgtac gtagcgcgcc cgcaccaagc
     2461 ggcggctatg atgtgctggc agaagttcgc ttagaggcat tggccgaagg cactgtgttt
     2521 tggaacaacc ttgcattaca aatacagccg ctaccttatt cgtttgatgc tgcaactcac
     2581 taatcacaat gggtaaattc ttcaacgttt ttctgttttt ttgaacgcta ggctgccaga
     2641 ataaatcttt tttattggaa tagtttttat aaaacattgg cactcacagc aatagcgtaa
     2701 cgaacttaaa atctattaat aaacgcccat ttacagtgca acctttttcg atgctctgcg
     2761 agccttataa ataaaggctt ccagagcata atttttttct cgaagcgaaa ttgaacgtaa
     2821 agttaaccat ttcaatcata aatattttta ataatttaac cccattgccg ccagttgacc
     2881 gtttaatcag caaatgccaa actgcaaaca tgcatgactt actttctgat gaagccctaa
     2941 tgcagcagtt ttgcgcaggt aaacaacaag cgtttgcgct actgtatgcg cggcatgaaa
     3001 agccgcttta ccgctatgta cggcgcgtgc tgggccaatc acttgccatc catgcggatg
     3061 acatttttca agatacgtgg atgaaactgg tagacgcaaa aagcacttgg caggcacaag
     3121 aaaacgccag ttttaaaact tggctattta ctttggcgca tcaccgtgct gtggatattt
     3181 tgcgcaaaaa tggccgtgaa atctctgtgg atgatggctg gctggatgat aacagtgaat
     3241 ccccttggca attatggccc gcttccaccg ccgaacaacc tgaacagcag gcgttttggc
     3301 gcaaggctgg gcaacaattg ttagattgtt tagaaggctt gcctgcactg cagcgcgctg
     3361 cctttttatt acaccatgaa gacggattaa gtttggatga aatgacacaa gtattgagtg
     3421 cagaatttga aactgtaaaa agccgcctac gctacgcgct tggcaaactg cgtgcatgca
     3481 tgggcgcata tctgcacccg atgatagaga aagaccaaag ctaatgcatc tgcaagacga
     3541 gcatctcaaa accgccttac agcacgcgcc agaccgtgat gtgcaaccta aagcccatgt
     3601 gcgtgatgct gtattgcgct atgcgaaaaa agccactgtg ccgcaaccaa gcaaacttca
     3661 acgcttgcgg tactggttat tttatggaaa actatctcaa gcgcaatggg ctggcatcgg
     3721 tagcgttgcc accgttctgc tggtggtgtt catggcgtgg caacagcatc ctgaagagca
     3781 cgtttggatt acggctgaaa cgaataacca aaatgcagca cacactgaaa tggccgccag
     3841 tgcgccagca ccagtcagtc aagatgctga aattacgcct gcaaccgccc cgcttgcaca
     3901 agaacaatta aatgatttcg cccaagaaaa acctatagca acgcaaaacg agcaggataa
     3961 atacgatagc gaaacgccaa ctaaacgtct caaaggcaat gtcatcgaag aaaaattgac
     4021 tggtaaaaag agcgtttcca aagatgctga gactgtaaaa ccactagaaa aagctgcaca
     4081 tgaagccagt cagacgagcg tgagtgaatc aaatgctgcc agcaaagaac taccggctga
     4141 attgagcgcg cagcatcagc ccagccctgt cgtagcagcc actgcaccac aagtgaatct
     4201 accagagagc aaaatcagct tgggcgcggc agataaatta gaaagccgcg ctattgcaaa
     4261 taaaacgcgc gaaaccttgg cgaacagcca aagtgccctt gttgagcaac tcatcacgca
     4321 gggtggccag aaagtcgctc atcaggatat taaaaatgag gtattacgca tactgaatgt
     4381 tgtggtgcat caagccgaag tacgcaatca ggacgcatgt acgcttaacc aaacaccatc
     4441 aacattagtt gatgcagcca ctggcttacg cattgaaaat atcgacattt gcgcagactc
     4501 agaacagtta caaaaagaag ttgaaagtta taataaaacg atgaaaagcg ggtatttgca
     4561 gtatcaaggc gaatagtaat ttgttacttt tctacattga gaaatgtccg agagaaaaaa
     4621 catgcgctgg aagcctttat tgataaggct cacagagcat cgaaaaaggt tgcaacttaa
     4681 attaaccctt taattgcgct attttgatga cgcgccgata tgacttggta aattttaaca
     4741 gacgtttatg gtgcgctgtt ttgtggcgct ggcgctgccg gcacttcatt ttggagcact
     4801 tggtaaaaga cttcatcttg cttaatcatg cccaactcgc ttctggcacg ctcttcaatg
     4861 gcagcgcggc cactttttaa atcgcgcact tcagcattga gcgcttcgtt acgcattttt
     4921 aacgcatcat ttttttcttg ctgcgcagtt atttgcgtat ttaaattcca cacacgcagc
     4981 caactgccct tgcccagcca cagcgggtac tgtagcaagg caatgaggac cacaaaaatg
     5041 agcgtcaggg ctttcattca gataaattca attgcaattg cattcaagct gagactaggc
     5101 gcacctttga agacagtaca attagtacgg caaaaaggcg caacgacgaa tcgggttgaa
     5161 agcatttgga attacttaaa tagctggtag aacgcacctt tgccagcata gctagaggca
     5221 tcgcccaact cttcttcaat acgcaagagt tgattgtatt ttgcaatacg ctctgaacgg
     5281 cataatgaac ccgttttgat ttgcagcgca ttggttgcca cagaaatatc ggcaatagtg
     5341 gtgtcttccg tctcgccaga gcgatgtgaa accactgccg tgtaacccgc acgtttcgcc
     5401 atttcaatgg tttggaatgt ttcagtcaag gtgccgattt ggttcacttt aatcaacact
     5461 gagtttgcca cgccgcgctg aatgccttca cgcagaattt ttgcgttggt gacaaacaag
     5521 tcatcgccca ctaattgcgt ggttttacct aaacgtttgg tgagaatttc ccaaccttcc
     5581 cagtcaaatt cacccatgcc gtcttcaatg gaaataatcg ggtatttatc acaccaagtg
     5641 gccaaataat cagtaaattg tgctgaagaa agtgctaaac cttctgattc taaatggtac
     5701 tttccatctt tgtagaattc ggtactcgca caatccaaac ccaaatacac atcacggccc
     5761 ggctcgtaac cagcatctga aatggattcc ataatcaact gaatcgctgc ctcattgcta
     5821 ggcaaattcg gtgcaaaacc accttcatca cccacggtag tagccaagcc ttttttatgc
     5881 aatgtttttt tcagttgatg aaaaatttct gcgccacaac gaattgcctc acggaatgat
     5941 ggcaaaccgg ctggaataat cataaattcc tgcatatcaa cggaattatc agcatgtgcg
     6001 ccaccattga tgatgttcat catcggggtg ggcaattgca tagcgccaga accgcctaaa
     6061 tagcgataca gcggcaaacc agactcttca gccgccgcgc gcgcacaggc cattgaaaca
     6121 cctaaaatcg cattcgcgcc aagtcggtct ttattttcag ttccatcaag ctcaatcaaa
     6181 gttttgtcga taaaactttg ttcttctgca tctagaccaa taatcgcttc tgtgatttct
     6241 gtattcacat tttcaaccgc ctgcaacacg cctttaccta aatagcggct tttatcgccg
     6301 tcacgcaact ccatggcttc ttttgcgcct gttgatgcgc ctgatggcac ggccgcgcgg
     6361 ccgataatgc cgctttcaag caacacatcc acttcaacgg ttgggtttcc gcgtgaatcc
     6421 agtatttcac gggcaataat atctacaata gcactcatgg ttttctccta attttcaaaa
     6481 aacgcgttca tttttatcac tgattgttat aaccaacgac cgttaacatc aacaactttg
     6541 caaggggttc caccttttcg gttctgttcg caatattcta ctgcttcttt ttttgcggcc
     6601 tcaatggttt catgatgtga gccactccag ccacatgatt cgtcacttga cgttgcaaac
     6661 gctttattgg aagagtaatc ccaataatcg taatattctt tcaggcaatt ggccgaaccc
     6721 aaatgctgca caatctgttt tttgcttggt ttattcaaaa tatcaatgat atccgatgct
     6781 ttttcaatca catcaccgca accagtgaga gatgtaagca gcaaaatggt caataatttt
     6841 ttcatgcgat tgcctataaa gtcatttcgg caaagcctgc ttttttaact aagcggtcta
     6901 agtcaattaa caccgccaac aaatcatgca tcttgcctaa aggccacgca ttagggccgt
     6961 cagacaaagc tttggctggg tcaggatgcg tttccataaa aatacccgca atgccactgg
     7021 ccaccgccgc ccttgccaac accggcacat gctcgcgctg accaccgctt ttatcccctt
     7081 gcccaccggg ttgctgcacg gaatgcgtgg catcaaacac taccgggcaa tgcgtttcgc
     7141 gcataatggc aagcccgcgc atatctgaaa ccagcgtgtt ataaccaaat gatacgccgc
     7201 gctcgcacac cataatatta tctgcgccgc cattggcttc acgggctttt tgcaccacgt
     7261 ttttcatgtc acccggcgcc ataaattggc cttttttgat attcacaggc ttaccagatt
     7321 ttgcacaggc ggtaataaaa tctgtttggc ggcacaaaaa agccggcgtt tgcaatacat
     7381 ccaccactgc tgccacatgc ggcacttgtt cttcagtatg cacgtcagtc aaaattggca
     7441 caccaatttg cgcgcgtacg tcatccaaaa tacgtaagcc ctcttccatg ccaaagccgc
     7501 gaaaagtctt gttcgagctg cgattagctt tgtcataaga tgacttataa ataaaaggaa
     7561 tacccaacgc gctagtgatt tcttttaatt gcccagccgt atcaattgcc atttgttttg
     7621 actcaatcac acacggccct gcgattaaaa acagcggatg ctcaagccca acatcaaagt
     7681 gacataattt catgctttag cctttacaga gagtgcgcct tttgatttgc caaggctgct
     7741 ttcacatatg cgataaacag tggatggcca ctacgtggat tagaggtaaa ttccgggtga
     7801 aactggcagg ccataaacca tgggtgtgtg gctttgggca gctcaatgat ttcacataaa
     7861 tcttcacgtg gcgttctggc agaaaccacc aagcccgcgg cttttaactg ttctacgtaa
     7921 tgattattca cttcataacg atggcgatgg cgctcattca cttgcgcacc ataaatgctg
     7981 gcggccatgg tgccttgtac aattgggcaa gcttgtgcgc ctaaacgcat ggtgccgcca
     8041 aggtcagagt tttcgtcacg cttctcgatt ttgcctgtgg catcttgcca ttcatcaatc
     8101 aagccgatca gtttatgcgg cgcgctggca ttaaattcgg tgctattggc acctgttagc
     8161 cctgccacat tgcgggcaaa ttcaattaca gccagttgca tgcccaaaca aatgcctaaa
     8221 taaggcactt tgttttcacg cgcatattga atagccaaaa ttttgccttc tgtaccgcgc
     8281 acaccaaagc cgcccggaat caaaatagca tccatgtttt ttaaactacc agtacctgat
     8341 ttttctaact cttcactatc aatatagtta attttaactt tagattctgt atgtatgccc
     8401 gcatgaatta acgcttcggt tagggattta tagctttcgg ttaaatctac atatttacca
     8461 acaaaagcaa tattgacgga atttttaggg ttttctaaac gtgtaacgat gtttttccaa
     8521 gccgttaaat cagctgcttt agctaaaata ttaagcttgt ggcaaacaat ttcatccatc
     8581 atttggtcgt gcaacagccc agggatttta taaatagagt cagaatcaat cgcgctaatc
     8641 actgcctcgt aagccacatt ggtaaacagc gcaattttgc gtttttcgtc ttcaggaatc
     8701 tcgcgctctg ccctacacag caaaatatct ggctgaatac caatttgccg caactctttt
     8761 acactatgct gagttggctt ggttttaagt tccccagcag ttggaatcca cggtaataaa
     8821 gtgagatgca cataacaagc attattgcga ccttcttcaa aacccatttg gcgaatggcc
     8881 tctaaaaatg gcagcgattc aatatcgccc accgtgccgc ccacttcgac aatcgcgaca
     8941 tccgcccctg ctgcaccacg cttgatatga tttttaattt catcagtaat atgcggaatc
     9001 acctgcaccg ttttgcctaa atattcacca cgacgctctt ttttaatcac ggtttcgtaa
     9061 atttggccag tggtaaagct atttcgccta gccatgcgct gcgaaataaa acgctcataa
     9121 tggcctaagt cgaggtcggt ttctgcgccg tcatctgtca caaacacttc gccatgctga
     9181 aaaggcgaca tggtgccggg gtcaacgtta atataggggt caagcttgag catggtcact
     9241 ttaatgccac gcgattcgag gattgcacct aggcttgcag ccgcaatgcc tttgccaaga
     9301 gaagaaacaa caccgccggt tacgaataca tatttggtca tggaaaggct ttacaagatt
     9361 tgttgcagac gggtataaat tttaccgcaa aggcgcttta ttcactagcg aaagttgcga
     9421 tgaattggca ataaaaaagc atccttgcgg atgcttttca tttaattaaa cttgctaaaa
     9481 aattaagctt gcgtttgtcg tcttgcgcta aaacccacta agaccaagcc agctaacaac
     9541 atggcatagg tttctggttc aggcaccgca gccaccgcta catttgaaaa gcctagtgtt
     9601 aagactgact ttgagctgtt tcctgagact gtgccactgg taaacaaatc gggcgagatg
     9661 tcagcaaaga cgttgtaagt tttgccaggt gtcacattaa agaacgccgt gccactacca
     9721 gaaaataact tatcaacggt agtgcctgct tcaaagttgt tatctacaaa tttcaattcc
     9781 tgttctgtgg caccaaaagc gtcttctaca atgctaaggc caatgaacat agcattgtag
     9841 gataattgac ctaccgttgg cgttgcaagg ttgataagag aactaaagtc ataagtgacg
     9901 gtgaggatat ttgaatctgc tacaaactga ttctccactg agacactcgg caccacgtaa
     9961 ctatattgtg aaccagcact tgtaccgcct ttatagcttg aaaaagcgtt gagtgacaac
    10021 tcaacaatat cgtacattga attcgataga tttttaatgc ctaaagccgc actcgccact
    10081 ccgttagcgc ttgtaaggct tgctgtgctt atattaggca agccagcaag actaaatgcg
    10141 gtcatgttta attgtggcaa actaacaggt tgttctggtt catcaatatc taagtaagca
    10201 ttaagattat atttttgtga cgtccagttt accgcgcccg catggctgag cgctggcgct
    10261 aaacttaaca gtacaaatcc tgcaatcgta ttgagtttca ctaaatgatt cctttttttt
    10321 gtttttatga cgggctaatt gtaacagtat ttatttttga aaatccacta taaattgata
    10381 aaaaatttac ataagtatca gaatttacac attttttagt cattttcgtt gttttgcaac
    10441 gcaaagttac ttattttcac cccagttacc atagccttta aattgctgaa aaacatcctg
    10501 catgtcttgc gtgcttaaaa tatgcggctt accaatttgc aatgtgcgcc aatattgtgc
    10561 gcacaagttt tctacctcca gcgccactgc cagcgcatca tctaaatcac gcccaagggc
    10621 tatcatgccg tggtttgcca gcaggcaggc tttgcgctca aataaggccg tcagtgctgc
    10681 atcagagagt gcttgcgagc caaacaaagc ataaggcgca cagcgaatat tgtcgccacc
    10741 ggccacggca atcatgtaat gaaacgcggg tatttcaata tgcaaacaag ccagcgtggt
    10801 ggcaaacatg ctgtgcgtgt gcaccaccgc gttgatttct gggcgatttt gcaaaatatc
    10861 acgatgaaaa cgccactcgc ttgaaggggt tttattcggt tcaacagtgc catcaaactg
    10921 catgcgcacc atgctattgg ccgacatcgc ctcagttgcc acgcctgaag gcgtaattaa
    10981 aaaagttgcc gcatttaagc gcacactgca attgcctgcc gtgcctacgt ttaagcctaa
    11041 cgcagtgagc ttttggctgg tacgcaataa ttgttcaaat aaatgttgca tgttatttct
    11101 cagtccattt ggcgcgtttt aagtgtattt ttgattcaat aaacgcttaa attcacacta
    11161 accttttttg atgctctgca agccttataa ataaaggctc gcagagcata tattttttct
    11221 caagatgaat atccttcttt atacatttta atcataagct gatttttggg tgtaatgccg
    11281 gctcgagttc cgctggcggg taaacaccat gctcggtcat aatccctgtc accagttgcg
    11341 ctggcgtaat atcaaaagcc gggttaaatg caggcgaacc tgctggcaaa atgcatacac
    11401 tttgcaagtg cccagcctca cttagccctt gcatgtggct gacctcctca cttgatcggt
    11461 gctcaatttc aatgagattt gcatcactga tagaccaatc aatagtgggg ctgggtacgg
    11521 ccgcgtaaaa cggaatatgg ttgtctttag ccgccagcgc acgtaaatag gtgccaattt
    11581 tattgcacac atcgcccaag gccgtcacgc ggtctgcccc cacaatcacc atatctactg
    11641 cgccaatttg catcaaatgg ccaatcgcgt tatctgccac aacggtatgt cgcacgccat
    11701 gctggcccaa ctcccaagcg gtgagactgg ccccttggtt gcgcgggcgc gtttcaccca
    11761 cccacacgtg cacgcgaagc ccggcatcat gcgcagcgta aataggcgcg agtgctgtgc
    11821 cgccatccac cgttgccagc caacctgcat tgcagtgcgt taaaatattt accacagctt
    11881 gatttgcttt gcgcgatgcc gcaatctgct gaataacgtt taagccgtgc tggccaatct
    11941 ggtggttcat ttgcacatct tcatcacaaa tacgggccgc ctcaagccag gcggccgact
    12001 tacgactttc tggcgacagc ggcaacaaca cattgcgcat acgctcaaca gcccagcgca
    12061 aattaagtgc ggtgggacga ctatggatta ataattgggc ggcttcttgt aaatcatcat
    12121 gatgcaacgc tgctagtgcc attccataag cagccgcagc gcctatgagc ggcgcgccac
    12181 gcacttgcat ggttttaatg gcgtgtagca tttgcggcaa tgtgctgatt tctgctattt
    12241 caaacacgtg cggcagttta gtttggtcaa taatgttaac aattggtttg tctgcacttg
    12301 tgtctgcact tggccaaatc gtacgatagt tttttccttc aattttcact tgacacgcct
    12361 aattttttta cgtttaaaat tgcacaaaaa tcacccaaaa gggctattca caatatctgt
    12421 ggataactct gtgaataact gtgactcaag aatgtaacat accaatttat aagggttttt
    12481 taaaatcgct taatttttga acttttattt ttatgtttta aatcagttag ttacaaacat
    12541 tccatgcttt tatcagggtt gctagccata gttaaagtag cacttgaagt gatgtgtata
    12601 actaagctaa ttttggcaaa atttgtagcg cattggcact agtaatatca agcacttgcg
    12661 acacatttac ttgacgtaaa tctgccaaaa actgggcgac tttaaccgtc tgagcgggcg
    12721 tatttctggc ctgttttccc agccattctg gcggaatatc gggtgaatct gtctctaata
    12781 cgattgcttc tagcggtaat tgttttgcca gcgcctgaat gtgcaacgca cggctatagg
    12841 tcattgcgcc accaaaaccc agtttaaaac ctaacgctat taactgttcc gcttgctgaa
    12901 aactaccgtt aaacgcatgc gcaatgccgc cattgacctt agctttacgc agatatttta
    12961 aaacatcgtc aatggcattt cttacatgca aaatcaccgg caaatgaaat tgattggcaa
    13021 tttttagctg ctcggtaaaa aaatatagtt gtttttcaat atgtgctggg tgttctttgg
    13081 cattttttac aaaataatcg aggccaattt caccaatcgc caccggttga tgcttggcca
    13141 cataagtttg aagcgttgct aaatcatctt ctgttgcgtg atccacatac atggggtgaa
    13201 cgcccaaggc aaaggcacaa tgggggtgtc gctggcaaac cgcaatcact gcatcaaaat
    13261 ttgcccgttc cacggctgga atcacaattt tttcaacccc ggcttgcagg gcagataacg
    13321 ccactgcatc gcggtcagca ttaaattcgg cagcatctag gtggcaatga gtgtcgatta
    13381 gcatcaaatt gaccgcttta cttttttggc ttggcgcgta agcgaatatc ttggccaaac
    13441 tgacgcacgt ctatgatctg caaatgcgtc acttgttgca tgttcgttaa ttcaggcaca
    13501 gcaaacatgc cttttgcgtc ggtacccatc agtttaggcg catagtacag gattagttca
    13561 tcaatcaagc cagcctgcaa gaacgcgcca ttcaaaccct gccccgcttc taacaacact
    13621 tcattcacgc cttgttgggc caaagttgca agtaacgccg ttaaatcaac gttgccgttt
    13681 tgatttggca actgcaacaa acgcacccct ttggccatga gcaattgctg ctgttgattt
    13741 gcatcattcg cataggcaat taacacgggg gctgcttgcg ggtggcttgt actcagcaaa
    13801 ttgcagtcta gtggcgtttg cagttggcta tcaacaatca cccgcaaagg ctggcgcgaa
    13861 acattggcta gccgcacatt catgcaagga ttatcggcca ataccgtgcc aatgccggta
    13921 ataatggcgc aagactgcgc gcgccagtgc tgtacatccg cccttgctgc ttcacctgta
    13981 atccattgac tttgatgatt gttaagtgcc gtgcggccat ctaagctcgc cgcaattttg
    14041 caacgcacat aaggcaagcc acgtgtcatt cgtgcaataa agcctgaatt caggttaaac
    14101 gccaccgatt ccataagccc taccgcaacc tcgatacc
//
LOCUS       DAOQSI010000038        14137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_3363_length_14137_cov_5.406547, whole genome shotgun sequence.
ACCESSION   DAOQSI010000038 DAOQSI010000000
VERSION     DAOQSI010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 14137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14137
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3363_length_14137_cov_5.406547"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..1273)
                     /locus_tag="PKL42_04025"
     CDS             complement(23..1273)
                     /locus_tag="PKL42_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNU66273.1"
                     /translation="MPTTLTVTFISLLIATTFIRIWLGRRHFLHVQQHRQAVPAAFSE
                     NITLSAHQKAADYTAAKTKLAMFEVVVQVALLLWLTLGGGLQWIDDIWRNLLPSQEII
                     RGAFVISSVMIVSSLIDLPFEYYRTFVVDEKFGFNKMTPAMFVTDLVKHTLVGLALGA
                     PILLVALWLMQGAGNYWWLYLWVIWSVFNLVMLAIYPTFIAPLFNKFTPLQDENLKQR
                     IEALLTKCGFKSQGLFLMDGSARSSHGNAYFTGFGASKRVVFFDTLLARLNADEIESV
                     LAHELGHFKHHHVIKRIALMFLVSFLGLALLGWLMNQPWFYTSFGVTQASNYMALVLF
                     LLVSPVFLFLLRPIMASYSRKNEFEADDYAAKHASAKYLVAALVKLYRDNASTLTPDP
                     LHSAFYDSHPPASIRISKLEAYTS"
     gene            1383..1943
                     /gene="orn"
                     /locus_tag="PKL42_04030"
     CDS             1383..1943
                     /gene="orn"
                     /locus_tag="PKL42_04030"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HNU66274.1"
                     /translation="MAESTNSSNQNNLIWLDMEMSGLLPDSDRILELAAVVTDADLNV
                     LGESPVLVIHQSDAVLDGMDAWNKGAHGRSGLIDKVKASTLDEEAATLQMIAFLKQFV
                     PAGKSPMCGNSICQDRRFMARYMPNLESYFHYRNLDVSVFKELARRWKPEIYAGFKKA
                     SKHEALADIYESIDELKYYREHFIIK"
     gene            complement(2067..3521)
                     /gene="glgA"
                     /locus_tag="PKL42_04035"
     CDS             complement(2067..3521)
                     /gene="glgA"
                     /locus_tag="PKL42_04035"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HNU66275.1"
                     /translation="MRVLFATSEVFPLIKTGGLADVSGSLPSALQHLGVDIRILVPGY
                     TAVLNALTDLHHLAHLAHLPVIGHAELLLGTIQETQVKVIVIKAAGIYERAGGPYADA
                     NGLEWLDNPVRFGVLSKVAAILGGPNSPLTDWHPNIVHCNDWQTGLTPAYMKLVEHAQ
                     AKSVISLHNMAFQGCYAPSWLPTLGLPSTHFNPEGLEYHGQLSFLKAGIFYADSITTV
                     SPSYAKEIQTPAFGFGLEGLLSKRGNELKGILNGIETKEWNPKTDPHLVKNYSATQLA
                     GKKQVKAALQERLGLEINADAPLLGVVSRLTHQKGLDMLAPIVPQLLDAGCQLALLGG
                     GESELENAFRHLARNHLGRVSVNIGYNEPLSHQIMAGSDMFIMPSRFEPCGLNQLYGL
                     AYGTPPVVNATGGLADSVIDTNIITYKNKTANGFVMSEASSASLLHCIKQALNVFKND
                     ADAWRQIQKNGMSQNLSWDKSALEYLAEYQKLLA"
     gene            3601..3984
                     /gene="crcB"
                     /locus_tag="PKL42_04040"
     CDS             3601..3984
                     /gene="crcB"
                     /locus_tag="PKL42_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNU66276.1"
                     /translation="MGFNGFLAVGAGAAIGAWARWGLGMLLNATWPALPLGTLVANLV
                     GGYFIGLLMGLFSLSTTVSPELRLFLITGLLGGLTTFSTFSVEAFTLLSRGQYGWATT
                     HILVHVIGSLVMTGLGFLTVNLIRG"
     gene            complement(4232..6898)
                     /locus_tag="PKL42_04045"
     CDS             complement(4232..6898)
                     /locus_tag="PKL42_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNU66277.1"
                     /translation="MKRMLFNATQEEELRVAIVDGQKLIDLDIEHAGKEQRKSNIYKG
                     VITRIEPSLEAAFVDYGQERHGFLPFKEVARSYYKEGADGKSRIQDALKEGQEVIVQV
                     DKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERDELRDVLAQLEVPK
                     GMSIIARTAGIGRNAEELQWDLNYLTQLWAAIDGASAMQAGAYLIYQEGSLVIRAIRD
                     YFSADIGEILIDTPDIHEQALQFMSHVMPGNVSRVKLYQDEIPLFTRFQIEHQIESAF
                     SREVRLPSGGAIVIDHTEALVSVDVNSGRATRGSDIEHTAFNTNLEAAEEVARQLRLR
                     DLGGLVVIDFIDMESQRNQREVENALRDALHADRARVQTGKISRFGLLELSRQRLRPS
                     LGETNHIPCPRCHGTGHIRGIESTALHILRITQEDAMKENSAIIQVQLPVEVATFLLN
                     EKRADIHAIEQRMGVEVVLIPNIHLETPNYNIVRVKHDDVTDETSRASYKLVELPTET
                     SYIRSPEETAKAVRPVAAVKGITPAAPAPIVAEKVAEPTPAPKLSLMARIKKFFGAPV
                     ESQPVQTPEAKKAAETRNEQNRNNRNRNRNRNDRNRGERADRPNAQDQQNRPADAKPN
                     EPRQQKPQQPRHEQQKNEAQRNSNQQTNVAQVEAIAAPNEATPQTTERTGRRNRNNRR
                     GRRDRDESAARPFVPNEALNANSATEAVAGTVAETQVQAAVISPVETEQNNNANAVVN
                     EQAQKNEAKAEKEVNAPHANIAEAIKAKRKTAKPTAAKEAVSNTAPANEGNTSETAVA
                     TADVEAAPAKANNKRPARTSKAKTSKAKAAAPVDLAASGLQLVETKVDAAKAVVLEEA
                     KPKAPRRAANWQKKANEEASAEPLVMVETQNK"
     gene            7611..8612
                     /locus_tag="PKL42_04050"
     CDS             7611..8612
                     /locus_tag="PKL42_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNU66278.1"
                     /translation="MQQVSALAAAFLTVDEASEGQRVDNFLTKMLKGVPKSHVYRILR
                     SGEVRVNKKRIDADFRLSLGDVVRIPPTRATQTAPADTIVPAVSKLNHTIIYEDDAML
                     VIDKPVGFAVHGGSGVSRGVIEQLRLERPKAKFLELVHRLDRETSGVLMLAKKRSALV
                     ALHEAIRNNQTDKRYLVLVQGEWTAPKKRVVLDLQRYLLPNGERRVNVVTDISKDKYD
                     ERQTSETLFRLLKNYQHPVLGHFSLLEAQLVTGRTHQLRVQLAHLGFVIVGDDKYGDF
                     ALNKTLQKNGLKRMFLHSATTKIRHPLTQEKMELTAPMPAELNQFLLKLTPATSVKN"
     gene            8790..9446
                     /locus_tag="PKL42_04055"
     CDS             8790..9446
                     /locus_tag="PKL42_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNU66279.1"
                     /translation="MPKQFDLIVWDWDGTLADSTGMITHAILKAAEQVGLPTISARAA
                     RNIIGLGLRESIHALYGDIPTEQAQALASQYSANYYAGEHEIPLFEGAADTITTLNKH
                     GFKLAVATGKGRRGLNSALEHCGLGNYFHATRTVDECFSKPHPQMLDELMDHLVVMPE
                     RTLMIGDTSYDLHMAQNAGVSAIGVSYGAQAAEQWQHLNPIGQFDDFATLSEWLFANA
                     "
     gene            9475..>9824
                     /locus_tag="PKL42_04060"
     CDS             9475..>9824
                     /locus_tag="PKL42_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNU66280.1"
                     /translation="MTEDSNIVNTIIFKSEELQNGALGLRFNLPQLGEFVTGFVVRFQ
                     DQPYAYVNQCAHVPVELDWNQGDFFTVQKDYLICSTHGAHYRPDNGFCVMGPCKGKSL
                     KPIPVTELNGQVII"
     assembly_gap    9825..9924
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9928..10884
                     /locus_tag="PKL42_04065"
     CDS             9928..10884
                     /locus_tag="PKL42_04065"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HNU66281.1"
                     /translation="MPEDNRWQRDTIEKLASSALTEQKTARRWGTFFKALTFIYLFTL
                     LFVAMGWFGGGKGHIGAHTALIEVSGVIQAGGDVNADSFMSSLRDAYEDKGTKGIILR
                     INSPGGSPVQAGIINDEIKRQKKLHPTIPVYAVVEDICASGGYYIAAAADKIYVDKGS
                     IVGSIGVLMDGYGFTEVMKKIGVERRLMTAGENKAMLDPFSPVNPKHQALAQAMLDQI
                     HEQFKTVVRQGRGNRLKETPETFSGLFWSGEESIKIGLADALGSADFVAREVIKQEEI
                     KDFTYQEDFASRIAKRVGAGASAAFGETLAKQMIEAGQVKLH"
     gene            complement(11012..11824)
                     /locus_tag="PKL42_04070"
     CDS             complement(11012..11824)
                     /locus_tag="PKL42_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HNU66282.1"
                     /translation="MKSLDQYRVNPKKFSLSQFRPDDKSSRSGNKAEDALTLTQLATE
                     INALQDVLHAEGKRKLLLVLQGMDASGKDGTVRHVFSECDPLGIRLASFKAPTSEELA
                     HDYLWRVHSQVPKAGELVIFNRSHYEDVLIVKVHDWINQAECQRRYAQINDFERMLTE
                     TGTTIIKCFLHISKEEQKIRMQERLNDPTKSWKFNVGDLKERALWPQYMQAYEDALKA
                     TSTDYAPWYVIPADSKTNRNLLISHLLRNTLKNMKSSYPATPAEYQSIVVED"
     gene            complement(11833..13485)
                     /gene="pgi"
                     /locus_tag="PKL42_04075"
     CDS             complement(11833..13485)
                     /gene="pgi"
                     /locus_tag="PKL42_04075"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNU66283.1"
                     /translation="MAALTTLPVWQKLCQHQKSMVSIHMRDLFAADKNRFDKYSLKLD
                     DILFDFSKHRINDETLPLLTQLAREANIEGWRDRMFAGEKINITENRAVLHTALRNRS
                     NTPVYVDGHDVMPDVNAVLAQMRAFSTKVRNGEWTGYTGKRITDIVNIGIGGSDLGPV
                     MVCDALKPYASPELQVHFVSNIDGAHLMRALEKCNPETTLFIVASKTFTTQETMTNAT
                     SARTWFLASAIDNAYVAKHFVALSTNAKAVQDFGIDINNMFAFWDWVGGRYSLWSAIG
                     LSIALYVGMDHFEALLTGAHEMDNHFKSAPLEQNIPVIMALIGIWYNNFFHVDTNAIL
                     PYDQGMARFPAYLQQADMESNGKFICRDGKRIQYKTGPVIWGEAGTNGQHAFYQLIHQ
                     GTQIVPCDFLMPVHSHYAIGKHGYAHHKILLANFLAQTQALMLGKTRDEARAELEKQG
                     LTGEALEQLLPHKVFEGNRPSTSILFDQVTPNTLGKLIALYEHKIFVQGMIWDINSYD
                     QWGVEYGKQIAQEILPLLTNDAVITNFDSSTNGLINYTKAHK"
     gene            complement(13517..>14137)
                     /locus_tag="PKL42_04080"
     CDS             complement(13517..>14137)
                     /locus_tag="PKL42_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HNU66284.1"
                     /translation="SHDEVVHGKHSMLDKMPGDAWQKFANLRLLLTYQMTSPGKKLNF
                     MGNEFGQGREWNVNSSLDWHLLSNEYAGHQWHQGLKLAVHDLNRLYQSHGALHTLDFE
                     VQGFEWIDCHDSDQSIVSYIRRGLDNSFVIIVLNFTPVLRQTYRIGVPVAGTYREVFN
                     SDANCYGGSNQGNGAGMHTENVAWMRHNQSLVITLPPLAGIVLQLN"
BASE COUNT         3670 a         3031 c         3315 g         4021 t
ORIGIN      
        1 atcgaaaaag gttgcggcct atttagctag tatacgcttc taatttagaa atacgaatac
       61 ttgctggcgg atgagaatca taaaatgcag agtgcaaggg gtctggcgtg agcgttgaag
      121 cattgtcacg atacaatttc accaacgctg caaccaaata tttggcactg gcgtgcttgg
      181 cggcatagtc atctgcttca aattcatttt tgcgtgaata gctcgccatg atagggcgta
      241 gcaaaaataa aaacacgggt gacaccaata aaaagagcac caatgccatg tagttgctgg
      301 cttgcgtcac accaaaactg gtgtaaaacc aaggctgatt cataagccat ccgagcaagg
      361 ctagccctaa aaagctcact aaaaacatca aggcaatacg tttaatgaca tgatgatgtt
      421 taaagtggcc cagttcatgg gcaagcaccg attcaatttc atcggcattg agccgcgcta
      481 atagcgtatc aaaaaacacc acgcgttttg atgcaccaaa accagtaaag taggcattgc
      541 catgactgct tctggctgag ccatccatca aaaataaacc ttgcgattta aaaccgcatt
      601 ttgtaagtaa ggcttcaatc cgttgcttga gattttcatc ctgtaacggc gtaaatttat
      661 taaataatgg cgctataaat gttggataaa tggctaacat gacgagatta aagacactcc
      721 agatgaccca taaatacaac caccagtaat ttcccgcgcc ttgcataagc cataaggcca
      781 ccagcaaaat aggcgcacca agtgctaaac ccaccaacgt gtgcttaaca agatctgtaa
      841 caaacatggc gggcgtcatt ttgttaaagc cgaatttttc atccaccaca aaagtacggt
      901 aatactcaaa tggtagatca atcaaactgc tgacaatcat gacgctgcta ataacgaaag
      961 caccgcgtat gatttcttga ctaggtaaga ggttgcgcca aatgtcatca atccattgta
     1021 aaccgccacc taaggtaagc cacaggagca atgccacttg cacgaccact tcaaacatcg
     1081 ctaacttggt tttagcggca gtgtagtcag cggccttttg gtgtgctgat aatgtaatat
     1141 tttcactaaa agccgcgggg acggcttggc ggtgttgctg cacatgtaaa aaatgcctgc
     1201 ggcctagcca aatgcgaatg aaggtggtcg caatcagtaa gctaataaaa gtaacagtta
     1261 gtgtagtggg cataatgtgt tcagttcggt taatatagtg ctattgtgaa gcaaaatttt
     1321 gcatttggtt cgcaccataa acgaaatggc gctttaattt tgatatttta atggaaatca
     1381 tgatggcaga atcgacgaat agcagtaatc agaataattt aatttggctg gatatggaaa
     1441 tgagcggctt gttgcctgat tctgaccgta ttttagaatt agccgccgtg gtgacagacg
     1501 ccgatttgaa tgtgttgggc gaatctcccg tgctggtgat tcatcaaagt gatgccgttt
     1561 tggacggcat ggatgcttgg aacaaaggcg cacacggccg ttctggctta attgacaaag
     1621 taaaggcttc caccttggat gaagaggccg ctacgctaca gatgatcgcg ttcttaaaac
     1681 agtttgtacc tgccggaaaa tcacccatgt gtggtaactc aatttgtcag gaccgacgat
     1741 ttatggcacg ttatatgcca aatttggaaa gttactttca ttaccgaaac ttggatgtga
     1801 gtgtgttcaa agagttagcc agaagatgga agccggaaat ttatgcagga ttcaagaaag
     1861 ccagcaaaca tgaggcgttg gcggatattt acgagtctat tgatgagtta aaatattacc
     1921 gcgagcactt tattatcaag tgattcagtc aaaaaaagag ggcgtctttt aggacgcccg
     1981 ggagggagaa aaaccagcct tggagaagct taattaaatt taagcattaa ctgtgccaat
     2041 tttctccctt acttgtttta cagattttaa gcgagtaact tttgatactc agctagatat
     2101 tccagcgcgc ttttatccca acttaaattt tgcgacatgc catttttttg aatttggcgc
     2161 caggcgtctg catcattttt aaagacattg agcgcttgtt taatgcagtg tagcagactt
     2221 gcggagctgg cttcgctcat cacaaaacca ttggccgttt tgtttttata ggtgatgatg
     2281 ttggtgtcaa tcactgagtc tgcaagcccg ccagtggcgt taacaaccgg tggcgtacca
     2341 taagctagac cgtacaactg gttgagtccg caaggttcaa aacgtgatgg catgataaac
     2401 atatcgctac ccgccatgat ttgatgagat agcggctcgt tataaccaat gttgacgctc
     2461 acacgcccta aatgatttct cgccaaatgg cgaaaggcat tttctagctc gctttcgcca
     2521 ccacccaaca gcgccaactg gcagcctgca tctaatagtt gcggcacaat gggggctagc
     2581 atatccaagc ctttttgatg tgttaagcgg ctgaccacgc ccagcaatgg tgcgtctgca
     2641 ttaatttcaa gccctaatcg ttcttgaagc gcggctttga cttgtttttt acccgccaat
     2701 tgcgttgcgc tataattttt aaccaaatgt gggtcagttt tcgggttcca ctctttggtt
     2761 tcaatgccgt tgagaatgcc tttaagctca ttgccccttt tactgagtaa accttctaag
     2821 ccaaaaccaa aagcaggcgt ttgaatctct ttcgcgtagc ttggactcac ggtggtgatg
     2881 ctatccgcgt aaaaaatacc cgcttttaaa aaggacaact ggccgtgata ttccagcccc
     2941 tcaggattaa agtgcgtgct gggtagccca agcgttggca gccaactggg cgcataacag
     3001 ccctgaaaag ccatgttatg caggctaatc actgatttag cctgtgcatg ctcaacaagc
     3061 ttcatatacg ctggggtaag gccagtttgc cagtcgttgc aatgaacgat attcgggtgc
     3121 caatcagtta aaggtgagtt tggaccgcct aagatagctg ccacttttga cagcacgcca
     3181 aagcgcacgg ggttatcaag ccattctaaa ccgttggcat ctgcatacgg gccaccagcg
     3241 cgctcataaa tgccggcagc cttaatgacg atgactttga cctgtgtttc ctgaatcgta
     3301 cccagcaaga gttctgcatg gccaatcaca ggtaaatgcg cgaggtgcgc gaggtgatgt
     3361 aaatcagtca gcgcatttaa tacagccgtg tagcctggaa ctaaaatgcg aatatccacc
     3421 cctaaatgtt gcaaggcaga aggcagagag ccgctgacat cggctagccc tcctgtttta
     3481 atgagcggaa atacttcaga agtggcaaat agaacgcgca aaatagtctt tcaataaaat
     3541 gaggtggatg cattattata tggcataaat tagttgctta atatcgtttt gggagtgggt
     3601 atgggattta acggattttt agcagtaggg gcaggggctg ccatcggcgc atgggcgcgc
     3661 tggggtttgg gaatgttgct caacgcaact tggcccgcgc taccattggg aacactggtg
     3721 gccaatttag tgggcggtta tttcattggc ttactgatgg gtttgttttc tctcagtacc
     3781 accgtttcac ctgagttaag attgttttta atcacggggt tacttggtgg cttaacaaca
     3841 ttttctacat tttcggtaga agctttcacg ctgttaagcc gtggccaata tggctgggcg
     3901 accacgcata ttttagtgca tgtgattgga tcgttggtga tgacgggctt gggatttttg
     3961 acggtgaatt taatccgcgg ttaatgcatt tttcgtatga ttttttcaga ggttttgatt
     4021 aactaaagcg tgaataatct atcgagaaaa aataaatgcc ctgtaggcct ttatttataa
     4081 ggctcgcagg gcatcaaaaa aggttgaagg taaaaatgac ataattgaac cattttattc
     4141 atgacacaat cagcaacaga aatcatcagt ttcaatacaa aaaagccctg aataacaggg
     4201 ctttttttag ctcaacacac acaaaatata attatttatt ttgtgtttcc accatcacca
     4261 aaggctccgc gctggcttct tcattcgctt ttttctgcca attggcggct ctgcgtggtg
     4321 cttttggttt tgcttcttca agcacaaccg cttttgcagc atctactttt gtttctacta
     4381 attgcaagcc agatgccgct aaatcaaccg gtgctgctgc cttagctttg ctggttttag
     4441 ctttgctggt gcgtgcaggg cgtttgttgt tagcctttgc tggggctgct tcaacatcgg
     4501 ctgtagcaac tgcggtttca cttgtgttgc cttcattggc aggagcggta ttgctcaccg
     4561 cctctttggc ggctgttggt ttagctgttt tacgcttggc cttaatcgcc tcagcaatat
     4621 tggcgtgtgg cgcattcact tctttttcag ctttcgcttc atttttttgt gcttgctcgt
     4681 ttaccacagc gttagcattg ttgttttgct ctgtttctac cgggctgatt actgccgctt
     4741 gaacttgcgt ttcagcaact gtgccagcaa cggcctctgt cgctgagttt gcatttagcg
     4801 cttcattggg aacaaaaggg cgtgctgcag attcatcacg gtcacggcgg ccacggcgat
     4861 tattgcggtt acggcggcct gtgcgctcgg tcgtttgtgg ggttgcctca tttggcgcag
     4921 caatggcttc aacttgcgca acgtttgttt gctggttgct gttgcgttgt gcttcatttt
     4981 tttgttgctc atgacgtggc tgctgcggtt tttgctggcg aggttcattc ggcttagcat
     5041 cggcagggcg attttgctgg tcttgcgcat tgggtctatc ggcacgctca ccgcgattgc
     5101 ggtcattacg gttacgattg cggttgcgat tgttacgatt ttgctcattg cgcgtttcgg
     5161 ccgctttttt ggcttcaggc gtttgcacag gctggctttc aaccggcgca ccaaagaact
     5221 ttttgattct ggccattagc gacaattttg gcgctggcgt tggttctgct actttttcag
     5281 ccacaatcgg cgctggtgct gctggggtaa tgccttttac tgcggcaacc ggacgcactg
     5341 cttttgctgt ttcttccggg cttctgatgt atgaagtctc cgttggtaac tccaccaatt
     5401 tatagctggc acgactagtt tcatcggtca catcatcatg ttttacacgc acaatattgt
     5461 agttaggtgt ttcaagatga atgttaggaa ttaacaccac ttcaacgccc atacgttgtt
     5521 caatggcgtg aatatcagca cgtttttcat tcagcaagaa agtggcgact tccactggta
     5581 attgcacttg aatgatggcg ctgttttctt tcatcgcatc ttcttgcgta atacgtaaga
     5641 tgtggagtgc ggttgattca atgccgcgga tatggcctgt gccatggcag cgtggacagg
     5701 gaatgtggtt agtttcaccc aaacttgggc gcaagcgttg acgtgacaat tcaagtaaac
     5761 caaagcgtga aattttgcca gtttgtacgc gcgcacggtc tgcgtgcagg gcatcacgca
     5821 aagcattttc aacttcacgt tgattgcgtt ggctttccat gtcaataaaa tcaatcacca
     5881 ccaaaccgcc caagtcacgt aaacgtaact ggcgagctac ttcttcagca gcttctaaat
     5941 tagtgttaaa tgcggtgtgt tcaatatcgc taccacgggt ggcgcggcca gagttaacat
     6001 caaccgaaac taacgcttcg gtgtggtcaa ttacaatggc accgccagaa ggcaaacgta
     6061 cttcacgtga gaatgcagat tcaatttgat gctcaatttg aaagcgtgtg aagagtggaa
     6121 tttcatcttg gtataatttc acacgcgaca cattgccagg catcacgtgg ctcataaact
     6181 gcaacgcttg ttcgtgaata tccggtgtgt cgattaaaat ttcgccaatg tctgcactga
     6241 aataatcgcg aatggcgcga atcactaagc tgccttcttg ataaatcaaa tacgcaccgg
     6301 cttgcatggc tgaagcgcca tcaatcgcgg cccaaagttg ggtgagatag tttaaatccc
     6361 actgcaactc ttcagcgtta cggccaatac ctgccgtacg ggcaatgatg ctcatgcctt
     6421 taggtacttc aagttgggct aatacatcgc gcaactcatc gcgctcttcg ccttcaattc
     6481 ggcgtgaaac gccaccgcca cgcgggttgt tgggcatcag cactaaataa cggccagcga
     6541 gtgaaataaa agtggtgagc gctgcgcctt tgttgccgcg ctcgtcttta tcaacttgca
     6601 cgatgacttc ttgaccttct ttcagcgcat cttgaatgcg agatttacca tctgcgcctt
     6661 ctttgtaata actgcgcgcg acttctttaa agggtaagaa gccgtggcgt tcttggccgt
     6721 aatcaacgaa agccgcttca agcgaaggtt cgatgcgtgt aattacgcct ttgtagatat
     6781 tgcttttgcg ctgttcttta ccagcgtgtt caatatcaag atcaatcagt ttttgtccat
     6841 cgacaatcgc tacgcgtaat tcttcttctt gcgtagcatt aaataacatg cgtttcatgg
     6901 tgtactcctg gctcaaggtc aggggcagac acaatgctta aagtataagc ttctaataaa
     6961 tttgagactt ttagtgaggt accaaatgat tggcacaacc tactgaaggc gcttgcaatg
     7021 cgcggggaaa aatttaacgt gttgaattaa aaaattcaag catcaatgaa atgaaaacaa
     7081 acgcgtgaga acacagcgtt gttttgcatt aaccgttcat gaaaaccagt ttcaaaatgt
     7141 gcttgctggc agttgcacag cgcggcatta ctttgcggtt ttgaatgaaa acttgttaat
     7201 tgtgacgtta aattaaacta atttattaaa tttacgcctg tggtggccta aattcgttaa
     7261 aattccatct tcagtaaatg ctaagttagc gctaagatta attactgcta agttagactt
     7321 tcactttaag tcgcgcttta tgttttttgt ctgcggcggc ttgagcgttc tttttacttg
     7381 ctgctacttt ataatatggc gagcggcatt aacccagtat ctgttttttt acttttaatt
     7441 acattaggtt gcgttcatct agaacgctat cttcaatgca attacgaaat tcgtaatgca
     7501 ctgatcgtgc caactaattt ataagtttaa ttggcacgcg taagtatagg ctactactca
     7561 catttaagca aagcatgaac acaattaaag cacaacatcc gcaagaaaaa atgcaacaag
     7621 tcagtgcgtt agccgcagct tttttaacgg tggacgaggc aagtgaaggg caacgagtgg
     7681 ataacttttt gacgaaaatg ctcaagggcg tgccaaaaag tcatgtctac cgtattttgc
     7741 gtagcggcga agtgcgcgtg aataaaaagc gaatcgatgc tgattttcgt ttaagtttgg
     7801 gagatgtggt gcgcattccg cccacacgcg caacgcaaac tgcgcccgca gacaccattg
     7861 tacccgccgt ttctaaactg aatcacacca ttatttatga agatgatgcg atgttagtta
     7921 ttgataagcc ggtaggattt gccgtgcatg gcggcagcgg ggtgagtcgc ggtgtgattg
     7981 agcagctgcg cttagagcgc cccaaagcta aatttctaga gttggtgcat cgcttggacc
     8041 gtgaaacttc tggcgtgctc atgttggcca aaaaacgcag cgctttagtg gccctgcatg
     8101 aggcgattcg caataaccaa acggataaac gttatttggt gctcgtgcag ggtgaatgga
     8161 ctgcgcctaa aaaacgcgtg gtgcttgatt tgcagcgcta tttactgccc aatggtgagc
     8221 gacgtgtgaa tgtggtcacc gatatcagta aagataaata tgatgagcgg caaacttctg
     8281 aaactttatt cagattgctc aaaaactatc agcacccggt gttaggtcat tttagtttgc
     8341 tagaggcgca acttgtcacc ggtcgtacgc atcaattgcg cgtgcagttg gcgcatttgg
     8401 gttttgtgat tgtgggcgat gacaaatatg gtgattttgc actgaacaaa actttacaaa
     8461 aaaatggtct taaacgcatg tttttgcatt ccgcgacaac taaaattcgc catccgctaa
     8521 cacaagaaaa aatggaactc acggccccga tgcccgcgga attgaatcag tttttattga
     8581 aactgacccc tgcaacatct gttaaaaatt gagcattgca aacagtcaaa aatggcctgt
     8641 tttaatcaca acctttttcg atgctctgtg agccttataa ataaaggctt gcagaggcga
     8701 aattttttct cgtacgtttt ttaatgtata tttaaataaa ttcttcatgt ttatttaatt
     8761 gttttttttg acacaaggta atatttttta tgcccaagca gtttgattta atcgtgtggg
     8821 attgggatgg tacattggcc gattccactg gcatgattac acatgccatt ttaaaagctg
     8881 ccgagcaggt aggtttgccg actatttcgg cacgtgcggc gcgcaatatt attggcttag
     8941 gcttacgtga gtcgattcat gcgttatatg gcgacattcc aacggaacag gcgcaagcgc
     9001 ttgccagcca atattcggcc aattattatg ctggtgaaca tgaaattcct ttatttgaag
     9061 gcgctgctga cacaattacc acgctgaata aacacggctt taaattagcc gtcgccactg
     9121 gcaagggccg aagagggctt aactctgcgc tggaacattg cggtttgggc aattattttc
     9181 acgccacgcg tacggtggat gaatgttttt caaaaccgca tccgcaaatg ctggatgaat
     9241 taatggacca tttagtggtg atgcccgagc gcactttgat gattggtgac accagttacg
     9301 atttacatat ggcgcaaaat gcaggcgtga gcgcgatagg cgtaagttat ggcgcacaag
     9361 ccgctgaaca atggcagcat ttaaacccga ttggtcaatt tgatgatttt gcgacgttaa
     9421 gtgagtggct gtttgcaaat gcctagccgg ttaatttgag cgaggatgga tgtgatgaca
     9481 gaagatagca atattgttaa taccattatt tttaaaagtg aagaattgca aaatggcgcg
     9541 cttggtttgc gttttaattt accgcagttg ggtgagtttg tgacaggctt tgtggtgcgt
     9601 tttcaagatc aaccgtatgc ctatgtgaac caatgcgcac atgtgccagt ggagttggat
     9661 tggaatcaag gtgatttctt tacggtacaa aaagattatt tgatttgttc aacgcacggc
     9721 gcgcactacc ggccggataa tggcttttgt gtgatggggc cgtgcaaggg caaaagttta
     9781 aaacccattc ccgtgaccga actaaatgga caagttataa tcaannnnnn nnnnnnnnnn
     9841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9901 nnnnnnnnnn nnnnnnnnnn nnnngctgtg ccagaagaca atcgttggca gcgcgacacc
     9961 attgaaaaac ttgcgagttc agcgttaact gagcaaaaaa ctgcgcggcg ctggggtacc
    10021 ttttttaaag cactcacgtt tatttatttg tttacattat tatttgtagc catgggttgg
    10081 tttggcggcg gtaaaggtca cataggcgca catactgcat tgattgaggt gtctggtgtg
    10141 attcaggctg ggggtgatgt gaacgcagat tcgtttatga gcagtttacg tgacgcctac
    10201 gaagacaaag gcacgaaggg cattatttta cgtattaaca gcccaggtgg cagcccggtg
    10261 caagctggca ttattaatga tgaaatcaaa cgccaaaaga aattacaccc gaccattccc
    10321 gtgtatgccg tggtagaaga tatttgcgca tcgggcggct attacattgc cgcagcggcc
    10381 gacaaaattt acgtggataa aggcagtatt gtgggttcga ttggcgtgtt gatggatggt
    10441 tatggtttta ccgaagtaat gaaaaaaatt ggcgttgagc gccgtttgat gactgctggt
    10501 gaaaacaaag ccatgctcga cccattttca cccgtaaacc caaaacatca ggcattggcg
    10561 caggccatgc tagaccaaat tcacgagcaa tttaaaactg tggtgcgtca gggtcgtggc
    10621 aaccgtttaa aagaaacgcc agaaactttt agcggcttgt tctggagcgg tgaagaaagc
    10681 atcaaaattg gcttggccga cgcgctgggc agtgcagatt ttgtggcacg tgaagtgatt
    10741 aaacaagaag agattaaaga ctttacctac caagaagatt ttgccagccg tattgcaaaa
    10801 cgtgtgggcg ctggtgccag tgccgctttt ggtgaaaccc ttgccaaaca aatgatagaa
    10861 gcggggcaag ttaaattgca ttaaatatag tctttaatat tgggccgtaa atgaatgcaa
    10921 cctttttcga tgccctgtga gccttatcaa taaaggctta cagggcattg tttttttctc
    10981 ggggaaaatt tacagcatta aaagcgaaat tttagtcttc aaccacaatc gattgatatt
    11041 cagctggcgt tgccggataa gatgatttca tgttttttaa agtgttgcgt aacagatgag
    11101 aaatcagcag attacgattg gttttagaat cagcaggaat cacgtaccag ggtgcgtaat
    11161 cggttgaagt tgctttgagc gcatcttcat aagcttgcat gtattgtggc caaagcgcgc
    11221 gttcttttaa atcccctacg ttaaatttcc aacttttagt cgggtcattt aagcgttctt
    11281 gcatgcgtat tttttgctct tcttttgaaa tatgcaaaaa acatttgatg atggtggtgc
    11341 cggtttcagt cagcatacgc tcaaaatcgt tgatttgcgc ataacgacgc tggcattccg
    11401 cctgattaat ccagtcgtgt actttgacaa tcagcacatc ttcatagtgg ctgcggttga
    11461 aaatgactaa ttctcccgct tttggcactt ggctgtgtac gcgccataaa taatcatgcg
    11521 cgagttcttc actcgttggg gctttaaagc tggcgagtcg aatgccgagc gggtcgcatt
    11581 cactgaacac atggcgcacg gtaccatctt tgccgctggc atccatgcct tgcaatacta
    11641 gcaatagttt acgtttgccc tcggcatgta aaacatcttg caaggcgtta atttcggttg
    11701 caagttgcgt gagtgtcagt gcatcttccg ctttattgcc tgaccgtgag cttttatcgt
    11761 ctggcctgaa ctgacttaac gagaactttt tagggttaac gcgatactgg tctaggcttt
    11821 tcatggcggc gtttacttgt gcgcttttgt gtagttgatg aggccgttgg ttgaactgtc
    11881 aaagttagtg atgaccgcat cgttggtgag taacggcaga atttcttgtg caatctgctt
    11941 gccgtactct acgccccatt ggtcgtaact gttgatatcc caaatcatgc cctgcacaaa
    12001 aattttatgc tcatataaag caattaattt acccagcgtg ttaggggtca cttggtcaaa
    12061 caaaatagaa gtactggggc ggttaccttc aaacacctta tgcggcaaca gttgctccag
    12121 tgcctcacct gttaagcctt gtttttctaa ctctgcacgc gcttcatctc tggttttacc
    12181 cagcatcagg gcttgtgttt gcgctaagaa gttcgcaaga agaattttat ggtgcgcata
    12241 accatgcttg ccaatcgcat agtgactatg cactggcatt aaaaagtcgc acggcacaat
    12301 ttgcgtgcct tggtgaatca gttggtaaaa cgcatgctgg ccattggtgc cggcttcacc
    12361 ccaaataaca gggcccgttt tatactgaat gcgtttgccg tcgcggcaaa taaatttgcc
    12421 attactttcc atatccgctt gctgtaaata ggctggaaag cgcgccatac cttggtcata
    12481 aggcaaaata gcattggtat ccacatgaaa gaaattgtta taccaaatgc caatcagcgc
    12541 cataatcacc ggaatatttt gctcaagcgg ggcggattta aaatggttgt ccatttcatg
    12601 cgcgcctgta agcaaggcct caaaatgatc catgccaacg tatagcgcaa ttgataagcc
    12661 aatcgctgac cacaatgaat agcgcccgcc aacccaatcc cagaaggcaa acatattgtt
    12721 gatatcaatg ccgaaatctt gcacggcttt ggcattggtt gaaagcgcaa caaaatgttt
    12781 tgccacatag gcgttatcaa ttgcggaagc taaaaaccaa gtgcgcgccg aagtggcatt
    12841 ggtcatggtt tcttgcgtgg taaacgtttt tgaggccact ataaacaggg tggtttcagg
    12901 attacatttt tccaatgcac gcattaaatg ggcgccatca atatttgaca caaaatgtac
    12961 ctgtaattct gggctggcat aaggtttgag tgcatcacac accatgacgg gccctaaatc
    13021 ggagccgcca ataccaatat tcacaatatc agtgatgcgt ttacctgtat agccagtcca
    13081 ttcgccattg cgtacttttg ttgaaaacgc acgcatttgc gctaacaccg cattcacgtc
    13141 tggcatcaca tcatggccat ccacataaac cggcgtatta cttcggttac gaagggctgt
    13201 gtgtaacacc gcgcgatttt cggtaatgtt gattttttca cccgcaaaca tgcggtcgcg
    13261 ccagccttca atgttcgctt cacgcgccaa ttgagtcagt aagggtaagg tctcatcatt
    13321 gatacgatgc ttggaaaaat caaataagat atcgtcgagt tttaaactgt atttatcaaa
    13381 tcgattttta tccgctgcaa ataaatcgcg catgtggatt gagaccattg atttttggtg
    13441 ctggcagagc ttttgccata cgggtagtgt cgttaatgca gccatttgat ttaagagtac
    13501 tttactttct agtaggttaa tttaattgta aaacaatgcc agctaaaggt ggcaaagtga
    13561 tgacaagtga ttgattgtga cgcatccatg caacgttttc tgtgtgcatt ccagcaccgt
    13621 ttccttggtt gctaccgcca taacagttcg catcactatt aaatacctct cggtatgtgc
    13681 ccgctacagg cacgccaatg cggtaagtct gacgcaacac aggcgtaaaa ttcagtacta
    13741 ttataacaaa actattatct aagccacgtc gaatatagct gacaatggat tggtcgctgt
    13801 catgacagtc tatccattca aagccttgca cttcaaaatc cagcgtgtgc agcgcaccat
    13861 gtgactgata gagtctgttt aagtcatgga cagcaagttt aaggccttga tgccactgat
    13921 gacccgcata ttcattactc aataaatgcc agtcgaggct gctattcaca ttccactctc
    13981 ggccttggcc aaattcgttg cccataaagt tgagtttttt gccaggtgaa gtcatttgat
    14041 aagttagcaa cagccgcagg ttggcaaatt tttgccatgc atcaccaggc attttgtcga
    14101 gcatagagtg cttgccatgc acaacttcat catgcga
//
LOCUS       DAOQSI010000039        13968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_3436_length_13968_cov_6.633724, whole genome shotgun sequence.
ACCESSION   DAOQSI010000039 DAOQSI010000000
VERSION     DAOQSI010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 13968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13968
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3436_length_13968_cov_6.633724"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PKL42_04085"
     CDS             complement(<1..478)
                     /locus_tag="PKL42_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNU66285.1"
                     /translation="MDISDLLAFSVKNKASDLHLSAGLPPMIRVHGDIRRINVPPMDH
                     TQVHDMMYDIMGDGQRKVFEETLECDFSFEIPNLARFRVNAFNHNRGAGGVFRTIPSK
                     ILSLEALNCPAIFKEISDTPRGLCLVTGPTGSGKSTTLAAMIDYINDNHFGHVLTVE"
     gene            612..1304
                     /locus_tag="PKL42_04090"
     CDS             612..1304
                     /locus_tag="PKL42_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNU66286.1"
                     /translation="MTSINSQLQEIHTRIQSVRAENGCPQPVYVLAVSKSKPAAAVRE
                     AYIAGQTMFGENYLQEALEKQAQLTDLPIEWHFIGPIQSNKTQAIAQHFSWVHSVDRL
                     KIAQRLNDARPIDLPPLQICIQVNISHEESKSGVQPEALFALASEISHLPQLRLRGLM
                     AIPAPTPDIEAQREQFKQVRRCYEALIAKGFDLDTLSIGMSDDYPIAIEQGATIVRIG
                     AAIFGARQLQST"
     gene            1407..2243
                     /gene="proC"
                     /locus_tag="PKL42_04095"
     CDS             1407..2243
                     /gene="proC"
                     /locus_tag="PKL42_04095"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNU66287.1"
                     /translation="MSNHIHFIGGGNMASAIIGGLKTNGFDMSNITVIEPDAEKRTLL
                     SATYGVQNLDTYVENLDSPSRQNIIVLAVKPQILQEVCQNLAKKISRQSSPLIISIAA
                     GVQCAAISRWLGGYSAIVRVMPNTPAQIQVGVSALFATSDVNDAQRLQAEQTLSAVGK
                     TLWLSDEQKMDAVTAISGSGPAYVFYLIEALQEAAMKLGLTLEEARLLSIETFAGASL
                     LAQQSTTDIQTLRAQVTSKGGTTEQGILALENANTKDIILAAAKAAAKRANELGILLG
                     KG"
     gene            2249..2809
                     /locus_tag="PKL42_04100"
     CDS             2249..2809
                     /locus_tag="PKL42_04100"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HNU66288.1"
                     /translation="MLNSIFIFLIKTILDFLAANFLLRFYLQRIQAPYQNPLSHSVIA
                     MTNFAIKPLRRIIPSLGKIDTSSLLAAFLSQLILYSTLAVSGNLPLISTFSKGAILLL
                     FSLVGLVKISLYLVMIVLIVYVILGWVAPHNPISEVLNTMTKPMLKTVRKYVPPVNFL
                     DLSPLIVGILAQIALIVMHHLVPLSL"
     gene            2929..5040
                     /locus_tag="PKL42_04105"
     CDS             2929..5040
                     /locus_tag="PKL42_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HNU66289.1"
                     /translation="MTNYANNTLTSPINPAPTSVQQNGLATASLSAKISEYSQWRDTL
                     IATIDEYANWLNNEDSIDSIQELRLYDIKDMLKKDQLVLAFLAEFSTGKTELINALFF
                     SDFNQRLLPSSPGRTTMCPTEIFWNELDEPSIRLLPMETRQTDDSITFLKTTPEIWHK
                     IRLDISSAESMKESLEVLAQKRNVDLATAKRLGFWNENDSTMTRSLAENGTVEIPAWR
                     HALVNYPHPLLKDGLVVIDTPGLNTMGAEPELTLSIIPSAHAVLFLTSTDTGITKSDM
                     QIWTEYVQKRASHKLVVLNKVDTLWDGLETPAEVDAIIYRQKQNTARELGLDARNVFA
                     MSAQKALLAKIRKDDALLARSGIGLLEDALANQLIDAKHEILGRAVITECSNMIRSTR
                     KIAQMRVKDSKSQLEELKAMQLKNRDSAKEILANVVAERKRYEASLLSFNQGSEKIKR
                     LGDKLLRHLSLGYLDTTLARTRQDMGDSWTTVGLNRSMKELTTLANNLAEEITLEGKT
                     IKQQADDLYHLFWSKHGFEKTEAIALDMSKFTKSMQMLEKVTSDFCSDPINILTEKHF
                     LIRRYYLGLGAQIQATFEEAHTDCTRWLNFVIDELKTQINSHKKSLDERAETLMQSHS
                     SADQLIQYLEKTEKEYYKLLQRSSELDGILLKLMRSTKFRPEKPKAAELGAPELNLSG
                     FNQATLSANAVLNNTQTQTLN"
     gene            complement(5149..6600)
                     /gene="rng"
                     /locus_tag="PKL42_04110"
     CDS             complement(5149..6600)
                     /gene="rng"
                     /locus_tag="PKL42_04110"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HNU66290.1"
                     /translation="MSEEILINVTPQETRIAMIENGVVQELQIERSSSLGLVGNVYRG
                     VVCRVLPGMQSAFVEIGLQRAAFLHAGDIFECSRSEPPLTIQSVLHEGQSLMVQVIKE
                     PIGTKGARLTTQISIAGRFLVFLPQQEHIGVSQRIEDEAEREQLRVRLIGLLPENYAG
                     GYIIRTMSETATDAELLADIDYLTKTWAHIQSKSTQVPERALIFQDLNLPMRALRDVL
                     CETTEAVRVDSSETYQKLVDFANVYAVTAVNKLMHYQGERPLFDLYNIEQEIENAMSR
                     KVALKSGGYLIFDQTEALTTVDVNTGSFVSGKNLSDTIFKTNLEAAQTIARQLRLRNL
                     GGIIIIDFIDMDEDSQREAVLEELQKEVARDRARTGVNGFTPLGLVEMTRKRTRESLA
                     HILCEPCTTCQGRGELKTAQTVCYEIMRELLKEARQFNARELRVLAAPKVIEMLLEEE
                     SQNLANLSDFIAKPISLQVEANYSQESFDIILM"
     gene            complement(6643..7230)
                     /locus_tag="PKL42_04115"
     CDS             complement(6643..7230)
                     /locus_tag="PKL42_04115"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNU66291.1"
                     /translation="MTSATLYLASRSPRRVELLKQLGLTCEIVPADIDESQLSHELPE
                     AYVKRLAQEKAVACLKNLSGAQRRHAVLAADTTVAIAGKVLGKPEDDQDARQMLQALS
                     GRTHQVHTAVALAFADAIDVVLSTTLVEMMELSSAQIEHYLASNEHRDKAGSYGIQGL
                     AGAWITRIEGSYTGVMGLPVYETANLLRKHGLIAL"
     gene            7347..7991
                     /locus_tag="PKL42_04120"
     CDS             7347..7991
                     /locus_tag="PKL42_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNU66292.1"
                     /translation="MTDWPALFKITIALFAIVNPIGSVPIFISATHGWSEADRAKTAK
                     TVAFTVFIVLAISAFFGDMILDFFGVTIPSFQVGGGILLMLIAISMMHGKQSGTRQTP
                     EEAQTLAEREVIAIVPLSIPLLAGPGAISNMIIAAQQNSSFAGHLSLIAPIFCISLII
                     WLVLKLAVVISKKLGTIGINIVTRLMGLILAAMAVEFIAHGLHGLFPALAVISP"
     gene            8074..8517
                     /locus_tag="PKL42_04125"
     CDS             8074..8517
                     /locus_tag="PKL42_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNU66293.1"
                     /translation="MQWMPHATVAAMIEDNGKFLLVEETTDRGNRFNQPAGHLEDNET
                     LLDAVVRETLEETAYDFTPEALLGVYHWKHAHNDTTYLRFAFIGKVGTHYHAQPLDDG
                     IIRAVWMTVDEIRNQAHLMRSPQVLTCIEDYLAGKQFPLSVLTHL"
     gene            8530..9639
                     /gene="mnmA"
                     /locus_tag="PKL42_04130"
     CDS             8530..9639
                     /gene="mnmA"
                     /locus_tag="PKL42_04130"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNU66294.1"
                     /translation="MNLNKVQKRRIVVGLSGGVDSSVTALLLKQKGYDVTGLFMKNWE
                     DDDTDEYCSSKQDLIDAVSVADKIGIDIEAVNFSAEYKDRVFANFLSEYKAGRTPNPD
                     ILCNAEIKFKAFLDHAMGLGADLIATGHYAQVRENPLQAGQYQLLKADDGSKDQSYFL
                     YRLNQAQLSKTLFPLGTYLKREVREIARQAGLINAEKKDSTGICFIGERPFQEFLSKY
                     LPREPGDIKTPEGKVVGKHEGLMYYTLGQRQGLKIGGSRESNGEPWFVAAKDMDKNEL
                     IVVQGHEHPLLLNDGLKAGQLHWISEEQPITNWVYAAKTRYRQPDAPCEIDKISADEV
                     EIKFGQKQWAITPGQSAVVYESNVCLGGGIITGAI"
     gene            9697..10443
                     /locus_tag="PKL42_04135"
     CDS             9697..10443
                     /locus_tag="PKL42_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66295.1"
                     /translation="MPYFLGSIHILIALFFAVHAIRTGRQMYWLLILFSFPLLGSAVY
                     FFAEYLPSSKVDRGVKQVSNIALQLLDPTRELREAKQAFELTPTVQNRMRLAAALDGA
                     GEYAEAVKQFDACLNGPFADDPEVVFGAAKAKFHNQQPQLAIPLLLDIRAKQASFRTE
                     ELSVLLAQSYAASGDNSNALKEFNYAADTFGSAETRLQFALWAVSANDIEKAKILRVT
                     LEKDWQHWNKHTRGLYKALFNQLDAALSAK"
     gene            complement(10581..10937)
                     /locus_tag="PKL42_04140"
     CDS             complement(10581..10937)
                     /locus_tag="PKL42_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66296.1"
                     /translation="MSANQLYLEMVLGAFKVLAAYGQVLLSFLFLFAAGLLFFRTKSI
                     PAGVFFGGLLITTLISFVIRIMIEFQWFESTIALQEKIQLLSMVGFVAMAIQVIGFLV
                     YVLSLPNVPNKNAENS"
     gene            complement(10977..11585)
                     /locus_tag="PKL42_04145"
     CDS             complement(10977..11585)
                     /locus_tag="PKL42_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HNU66297.1"
                     /translation="MKLFYTVNSPYARKVRIVALEKRIQIGLLEVVLADPDCPVKQYN
                     PLGKVPVLVLPDGESLYDSRVIVEYLDNRTPLAHLIPQDPTAKIKVRRWEALADGVCD
                     AAVSVMLEQRKPEAQQSSAHIEKQFDKVTRGLNVLNLDLAKKKWCVNEKFSLADIALG
                     CALGYLDLRFAHLNWQESYPNLAKHYNVLVKRASFKQTMPVA"
     gene            11742..12617
                     /gene="nadC"
                     /locus_tag="PKL42_04150"
     CDS             11742..12617
                     /gene="nadC"
                     /locus_tag="PKL42_04150"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNU66298.1"
                     /translation="MTLFSQHYPPFSSAFYALVDSQVQAALAEDVGTGDLTAALVPAE
                     QHVTASIIAREPAIICGQDWVNACFLQTDANIHIQWLITEGEHVQPKQVLCKITGPAR
                     ALLTAERCALNFLQTLSATATHTRRYVDAITGTQAQIFDTRKTLPHLRLAQKYAVTVG
                     GGQNQRLALYDGILIKENHIAAAGGIAEVMTAAQAASSGKSVQIEVENLKQLEEALVA
                     GATSVLLDNFSPALLTEAVALNVQLGKKALLEASGGITLDNVREIALTGVDRISIGAI
                     TKNVIAIDLSMRIQH"
     gene            12721..>13968
                     /locus_tag="PKL42_04155"
     CDS             12721..>13968
                     /locus_tag="PKL42_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNU66299.1"
                     /translation="MNKPVVLSNPTGRLTLGDVLRMLVNDGMVDKVQAEKLYKDRKLD
                     SSKLHPLVVIGEQKWKNLLPPHKAFTVEALSHWLAARAGLDFYHIDPLKLDFGAAAKL
                     FAQGYAERLKIMPIGAKNGEAIIATTEPFSTEWIPDLERLLKMKVKLVVANPLEVSRY
                     LPEIYNLAHSMSAADASKAGQIVGVQNFEQLVELGKDKNLDANEQHVVNIVDWLFKYA
                     FEQRASDIHLEPRRGMGFMRFRIDGVLHQVYQLPPNIMNAITSRIKLLGRMDMVEKRR
                     PQDGRIKTLAANGQEIELRLSTMPTAFGEKLVMRIFTPEVSASDFLTLGFSKAQAATW
                     NTWTKAPNGIILVTGPTGSGKTTTLYATLRQLATPEVNVCTVEEPIEMVEPAFNQMQV
                     QTNIGLNFADCIRTLMRQDPDIIM"
BASE COUNT         4004 a         3301 c         3096 g         3567 t
ORIGIN      
        1 cctctaccgt taacacatga ccaaaatgat tgtcattgat atagtcaatc atcgctgcca
       61 atgtcgtgga cttacctgaa cccgttggcc ctgtcactaa acaaaggcca cgcggtgtgt
      121 cagaaatttc tttaaaaata gctgggcagt taagcgcttc taaagataag atctttgacg
      181 ggatggtacg aaaaacgccg ccagcaccgc gattatgatt aaatgcattg acgcgaaagc
      241 gcgccaaatt aggaatttca aatgaaaagt cgcattccag tgtttcttca aagactttac
      301 gttgcccatc gcccatgatg tcatacatca tatcgtgcac ttgggtgtgg tccataggcg
      361 gcacattgat acggcgaata tcaccatgca cacgaatcat cggtggcaaa cctgcagaaa
      421 gatgtaagtc cgaggccttg ttttttactg aaaacgcaag taaatctgaa atgtccacaa
      481 ctggctcctg taataattga tgccgatgtt actaaaaatg atttaaaatg gctatgacga
      541 tactgtatgt taatcaagaa gttatttcaa gatgttaatt tactctataa caaatattaa
      601 ccaactgcat tatgacctca atcaatagcc aactgcaaga aattcacacg cgcattcaat
      661 cagttagggc tgaaaatggc tgcccacagc ccgtgtacgt attggcggtg agtaaatcca
      721 aaccagcggc ggctgtgcga gaggcttata ttgccggcca aaccatgttt ggagagaatt
      781 atttacaaga agcgctcgaa aagcaggcac aattgactga cttgcccatt gaatggcatt
      841 ttattggccc aattcaaagt aacaaaacgc aggccattgc ccagcatttt tcttgggtac
      901 atagtgtaga tcggctaaaa attgcccaac gattaaatga tgctaggccg attgatttgc
      961 cgccattgca aatttgcata caggtcaata ttagccatga ggaaagcaaa agcggcgttc
     1021 agccagaagc actatttgcc cttgcaagtg aaatttcgca tctcccccaa ttaagactac
     1081 gcgggctgat ggcgattcct gcaccaacac cagatattga agcgcagcga gaacaattta
     1141 aacaagtgcg gcgttgctat gaggcgctta tcgcaaaagg atttgaccta gatacacttt
     1201 cgattggcat gtcagacgat tacccgattg ccatcgaaca aggtgccacc attgtgcgca
     1261 ttggtgctgc catctttggc gcaaggcaat tgcaaagtac ttaattaaat tgatacatgt
     1321 ttaaattgat acatagcaat tacatagaca ttacagcgct acatcaatat gataaccata
     1381 accatttaac aaacagactg ggcaacatga gcaaccatat tcactttatt ggcggcggca
     1441 acatggcaag cgccatcatt ggcggcttaa aaaccaacgg ttttgacatg tctaatatca
     1501 ccgtgataga accggatgct gaaaaacgga cactactcag cgctacttat ggtgtacaaa
     1561 atctcgacac ttatgtcgag aatctcgaca gtccatcccg tcaaaatatc atcgttttag
     1621 cagtgaagcc gcaaatttta caagaagttt gtcaaaacct cgctaaaaaa atatccagac
     1681 aatcgtcacc gcttattatt tcaattgcag ccggtgtgca gtgtgcggct atttcacgtt
     1741 ggcttggcgg ttactctgcc attgtgcgcg tgatgcccaa cacccccgca caaatacagg
     1801 tgggtgtttc tgcgctattt gccacttcag acgtaaatga tgcgcagcgc ttgcaggctg
     1861 agcaaacttt atcggccgtc ggaaaaacat tatggttaag tgatgagcaa aaaatggatg
     1921 cagtaacggc gatttctggc agcggtcctg cttatgtgtt ctacttgatt gaagccttac
     1981 aagaagcggc aatgaagctt gggctgacct tggaagaagc gcgcttattg tcgattgaaa
     2041 cattcgcagg cgcgagtttg ctcgcccaac aaagcacaac cgatattcag accctgcgcg
     2101 cgcaagtgac gtcaaaaggg ggcaccacag aacaaggtat tctagcactt gaaaatgcca
     2161 ataccaaaga cattatcttg gccgcagcca aagctgcagc caagcgcgct aacgaactgg
     2221 gcattctgct aggaaagggc taatcgttat gctgaattct atttttatat tcttaattaa
     2281 gacgatactt gattttttag ctgcgaattt tttgctgcgt ttttacctgc aacgcattca
     2341 ggcaccttac caaaacccgc tttcgcatag cgtaattgcc atgactaatt ttgcaattaa
     2401 acctttgcgt agaatcattc ctagcctagg aaaaatagat acttccagtc ttttggcagc
     2461 atttctcagc caactcatcc tctatagcac actggctgtt agcggcaact taccgctcat
     2521 ttcaacgttt tcaaaaggcg ccattttgct gctattttcc ttggtagggc ttgtaaaaat
     2581 cagcttatat ttggttatga ttgtgctgat tgtttacgtg attttgggct gggtcgcacc
     2641 gcataaccct atttctgaag tcttaaacac aatgacaaaa ccaatgctaa aaacagtaag
     2701 aaaatatgtt ccaccggtta attttttaga tttgtcgccg ttaatagtag gcatactcgc
     2761 gcagattgca ttaattgtga tgcatcatct tgtgccttta tcgctttaat ttgtaataac
     2821 aaaaactaaa gccaaatcac ggcacagtaa ttgcttacac agaggttgta cccatgtttc
     2881 aacgacttgt tatcgacttt atttagtgaa ttgaagacag cgctcaatat gacaaactat
     2941 gcaaataata ctttgaccag ccccatcaac cctgcaccaa ctagcgtgca gcaaaacggc
     3001 ttggctacgg ccagcctgag tgcaaagatt tcagagtact cgcaatggcg agatacgctg
     3061 attgcaacca tagacgaata cgcaaactgg ctcaataatg aagacagcat tgattcgatt
     3121 caagaattac ggctatacga catcaaagat atgttaaaaa aagatcaact cgttttggca
     3181 tttttggcgg agttttctac cggtaaaacc gaactcatca acgcgttatt cttttcagac
     3241 tttaatcaac gtctattgcc cagctcaccc ggccgcacaa caatgtgccc aacagaaatt
     3301 ttctggaacg aactggacga accttcgatt cgccttttac cgatggaaac ccgccaaacg
     3361 gacgactcca ttactttttt aaaaaccaca cctgaaatct ggcacaaaat ccgtttggac
     3421 atttcatcag ccgagtcgat gaaagaatca ctggaagtgt tggcacaaaa gagaaatgtt
     3481 gatttagcca ccgccaaaag actgggattt tggaacgaaa atgacagtac gatgacccga
     3541 tcgcttgctg aaaatggcac cgttgaaatt ccggcatggc gccatgcatt ggtaaactac
     3601 ccgcacccac tgctaaaaga tggcttagtg gtgattgata caccgggcct gaataccatg
     3661 ggtgccgaac ctgaactgac gcttagcatc atccctagcg cgcacgccgt gttgttttta
     3721 acctcaacag acactggcat cacaaagtct gacatgcaga tttggacaga atacgtacaa
     3781 aaacgcgcct cgcacaaatt ggtggtgctt aataaagtgg atactttgtg ggatggtttg
     3841 gaaacacctg cagaagtgga tgccattatt taccgacaaa aacaaaatac cgcacgcgaa
     3901 ctggggcttg atgcgcgtaa tgtgtttgcc atgtcggcgc aaaaagcctt gcttgctaaa
     3961 atcagaaaag atgacgccct actagcacgt agtgggattg gcttgcttga agacgcactc
     4021 gcaaaccagc tgattgatgc aaaacatgaa attctaggcc gcgccgttat tacagaatgc
     4081 tcgaacatga ttcgctctac gcgtaaaatt gcgcaaatgc gcgtaaaaga cagcaaaagc
     4141 caactagaag aattaaaggc tatgcagctc aaaaaccgtg attctgccaa agaaattttg
     4201 gccaacgtgg ttgcagagcg caaacgttac gaagcttcac tgttgtcatt taaccaaggc
     4261 agtgaaaaaa tcaaacgctt gggcgataaa ttattacgcc atttaagctt gggttattta
     4321 gacactacgc tagcgcgcac gcgccaagat atgggcgaca gctggacaac ggttggtctg
     4381 aacagaagca tgaaagaact caccacgctt gcaaataacc ttgctgaaga aattaccctt
     4441 gaaggcaaaa ccattaaaca gcaagcggat gatttatatc atttattctg gagcaaacac
     4501 ggctttgaaa aaaccgaggc cattgcgctc gatatgagta aatttacaaa atccatgcag
     4561 atgcttgaaa aagtgacttc tgatttttgt agtgacccca tcaacattct gacagaaaaa
     4621 cacttcttga ttcgccgcta ttatttaggc ttaggcgcgc aaatacaggc aacttttgaa
     4681 gaagctcata cagactgtac gcgctggctc aactttgtga ttgatgaact caaaacacaa
     4741 atcaacagcc acaaaaaatc acttgatgag cgtgcagaaa cattgatgca atcacacagc
     4801 agcgccgacc agttgataca atatttagag aaaactgaaa aagaatacta caagctactt
     4861 caacgctcga gtgaattgga tggcattttg ctgaagctca tgcgctctac caaatttagg
     4921 cctgaaaaac cgaaagcggc tgaattaggc gcgccagaat taaatttaag cggctttaac
     4981 caagcgacat tgtcagccaa tgcagtgctc aataacacac aaacacagac ccttaattaa
     5041 cccaaccctt ttgcatgcct ctggaagcct ttatttataa ggctcgcagg gcagaaaaat
     5101 tctctcggct ttaaaatact gcttttagca tgatttattt gcgatgtttt acatcaaaat
     5161 aatatcaaac gactcctggc tgtaattcgc ttccacttgc aaagaaattg gtttagcaat
     5221 gaaatcggat aaattggcca gattttgtga ttcttcttcc aataacattt caattacttt
     5281 gggcgcggct aatacgcgca attcacgcgc attaaactgg cgcgcttctt taagcaactc
     5341 gcgcataatt tcataacaca cggtttgtgc tgttttaagc tcgccgcgcc cttggcaggt
     5401 tgtgcatggc tcacacaaaa tgtgcgctaa actctcacgc gtgcgtttgc gcgtcatttc
     5461 tactaaaccg agtggcgtaa acccgtttac gcctgtgcgg gcacggtcgc gggcgacttc
     5521 tttttgcaac tcttccagca cagcttcgcg ttggctatct tcatccatat caataaaatc
     5581 gatgataatg atgccgccca agttgcgtaa tcgtaactgg cgcgcaatgg tttgcgccgc
     5641 ttcaagattg gttttaaaga tggtgtcgga aagatttttg ccactgacaa aactgccggt
     5701 attcacatcc accgtggtga gcgcctcagt ttggtcaaaa atcaaatagc cgcccgactt
     5761 taacgccact ttgcgcgaca tggcgttttc aatttcttgc tcgatattat ataaatcaaa
     5821 cagcggtcgt tcaccctgat aatgcatcag tttattcacc gcagtcacgg catagacatt
     5881 ggcgaaatcc accaattttt ggtaggtttc actcgaatcc acccgcacgg cttctgtggt
     5941 ttcgcacagc acatctcgca gggcgcgcat gggtaaattc aagtcctgaa aaatcaatgc
     6001 gcgctcgggc acttgcgtac ttttagattg aatgtgcgcc caggttttag ttaaataatc
     6061 aatatcggcc agcaattcag catctgttgc agtttcagac atggtacgaa taatatagcc
     6121 gcctgcataa ttttcaggca gcaagccaat cagacgtact ctaagctgct cgcgctcggc
     6181 ttcatcttca atacgttgcg acacaccaat gtgttcttgc tgtggcaaaa acactaaaaa
     6241 ccgcccggcg atgctgattt gcgtggtaag ccgtgcgcct ttggtgccga tgggttcttt
     6301 aattacttgc accatgagcg actggccttc gtgcagcaca ctttgaatag ttaaaggtgg
     6361 ttcggagcga ctgcactcaa aaatatcgcc tgcatgtaaa aatgctgcac gttggaggcc
     6421 aatttcaaca aaggccgact gcatgccggg caacacgcgg cagacgacgc cgcgataaac
     6481 attgccgacc aagcctaatg atgaactgcg ttcaatttga agctcttgca ccacgccatt
     6541 ttctatcatg gcgatacggg tttcctgcgg ggtgacgtta attaaaattt cttcgctcaa
     6601 tgtgtactca cttacttcat ttttttaaat cgaatgacat gactatagcg caatcagccc
     6661 gtgttttctt agcagattgg ccgtttcata gacgggcaag cccatcacac cggtatagct
     6721 gccctcaatg cgggtaatcc atgcgcccgc caacccttgt atgccataac tgcctgcttt
     6781 atcacgatgc tcgtttgagg caagataatg ttcgatttgc gcagatgaca attccatcat
     6841 ttcaacaagc gttgtcgaaa gcacaacgtc aatcgcatct gcaaaagcca gcgctaccgc
     6901 agtatgcact tgatgggtac gccccgataa tgcttgtaac atttggcgcg catcttggtc
     6961 atcttcaggt ttacccagga ctttacccgc gatggcaacc gtagtatcgg ccgccaaaac
     7021 cgcatgcctg cgttgagcgc cagataagtt ttttaagcaa gctacggcct tttcttgggc
     7081 aagccgcttc acatacgctt ctggcaactc atgacttaac tgtgactcat caatatcggc
     7141 tggtacgatt tcacaagtaa ggcctaactg ctttaataat tccacacgtc gtgggctgcg
     7201 cgaggcaagg tagagagttg ctgatgtcac gataatattt cacatattta ttttattaag
     7261 tcagcacttt aatttactat gagtcaaatg acaaagattt cgtgatttaa gtttagggca
     7321 cctctatata attcaaacgg agtgatatga cagactggcc tgcacttttt aaaattacca
     7381 ttgcattatt tgccattgtt aacccaattg gcagcgtgcc tatttttatt agcgccacgc
     7441 atggctggag tgaggctgac cgtgccaaaa cagccaaaac agttgcattc acggtgttta
     7501 ttgtgctggc catttccgca ttttttggcg atatgattct cgactttttt ggcgtcacca
     7561 ttccgtcttt tcaggtcggc ggtggaattt tgttaatgct gattgccata tcaatgatgc
     7621 acggcaaaca aagcggcaca agacaaacac ccgaagaagc gcaaacgctg gcagagcgtg
     7681 aagtgattgc cattgtgcca ttaagcattc cgctacttgc aggtccgggt gccattagca
     7741 atatgattat tgccgcacaa caaaatagca gctttgccgg gcacctttct ttaattgcgc
     7801 ccattttttg catcagcctg attatttggc tggtgttgaa actagccgtg gtgatttcaa
     7861 aaaaacttgg caccatcggc atcaacattg tcacccgcct catgggcctc attttagcgg
     7921 ccatggcggt ggagtttatt gcacacggtt tgcatggttt atttccagcg cttgctgtta
     7981 tcagcccgta aaaccacaaa gaacattcaa cccaagcgca attgagcgct tcgagttaaa
     8041 atagcgctta tatcaatatt caagaccaat accatgcaat ggatgccaca tgccaccgtc
     8101 gcggcgatga ttgaggacaa cggaaagttt cttttggtgg aggaaaccac cgatcgcggt
     8161 aaccgtttta accagcctgc gggccattta gaagacaatg aaaccctgct agatgccgtg
     8221 gtacgcgaaa cgctggaaga aactgcatac gactttacgc cagaagccct gcttggtgtt
     8281 tatcactgga agcatgcaca caacgacacc acatatttac gttttgcctt tattggcaag
     8341 gttggcacgc attatcacgc acagccgctg gacgacggca ttattcgcgc tgtgtggatg
     8401 accgttgatg aaatccgcaa tcaggcgcat ttaatgcgta gcccgcaagt gctaacgtgt
     8461 attgaagact atttggcagg caagcaattt ccgttatcgg tactgacgca tttatagaga
     8521 agactcaaca tgaatttaaa caaagtgcag aaaagaagaa ttgtcgtcgg cctgtcaggt
     8581 ggcgtagatt caagcgtgac agctttattg ctcaaacaga aaggctatga cgtgactggc
     8641 cttttcatga aaaactggga agatgacgac acggatgaat actgttccag caaacaggat
     8701 ttgattgatg ccgtatcggt ggcagataaa attggcattg atattgaagc agtgaatttt
     8761 agtgccgaat acaaagaccg cgtatttgcc aattttttaa gcgaatataa agcaggccgc
     8821 acacctaacc ctgatatttt atgcaatgcc gaaattaaat ttaaggcatt tttagaccat
     8881 gcaatgggtt tgggggcaga tttaatcgcc accggccatt atgcacaagt gcgcgaaaac
     8941 ccgttgcagg cagggcaata tcagttgctg aaagcagatg acggcagcaa agaccaaagt
     9001 tatttcttat atcgcttgaa ccaagcgcaa ttgagcaaaa ccttgttccc tttggggacg
     9061 tatcttaagc gagaggtgcg cgaaatcgca cgacaagccg gcttaatcaa tgctgagaaa
     9121 aaagattcca cgggcatttg ctttattggc gagcggcctt ttcaggaatt tttaagcaaa
     9181 tatttaccac gtgaacccgg tgacattaaa acaccggaag gcaaagtggt cggcaaacat
     9241 gaaggcctga tgtactacac cctcggccag cgccaaggct taaaaattgg cggaagccgt
     9301 gaaagtaacg gcgagccgtg gtttgtggcg gcaaaagaca tggataaaaa tgagctgatt
     9361 gttgtgcaag gtcatgagca tccactttta ttaaatgatg gcctaaaagc tggacaactg
     9421 cactggatta gcgaagagca gcccatcacc aattgggtgt atgccgctaa aacacgctat
     9481 cgccagcccg acgcaccgtg tgagattgat aaaatttcag ccgatgaagt ggaaattaaa
     9541 ttcggccaaa aacaatgggc cattacgccg gggcaatcgg ccgtggtgta tgaaagcaat
     9601 gtttgcctgg gcggtggcat tatcaccggc gcaatctgat taaaagttcg gtggtattat
     9661 gatgtcagcc atttaacgtc acgtgaaagt aagtacatgc catatttttt aggtagcatt
     9721 cacattttaa tcgcgctgtt ttttgccgtg catgccatcc gcaccggccg ccaaatgtat
     9781 tggttgctca ttttattttc attcccctta ttgggcagcg cggtgtattt ttttgcagaa
     9841 tatttaccca gctcaaaagt ggatcggggc gtaaagcaag tatcaaacat tgccttgcaa
     9901 ctgcttgacc ccacgcgcga gctacgcgaa gccaagcaag cctttgaact cacccccacg
     9961 gtacaaaaca gaatgcgcct cgctgccgca ctcgatggcg ccggggaata tgcagaagcc
    10021 gtaaagcagt ttgacgcttg cttaaacggc ccatttgcag atgaccctga agttgttttt
    10081 ggcgcagcaa aagccaagtt tcacaaccag caaccgcaac tcgctattcc attattgcta
    10141 gacattcgcg ctaaacaagc cagtttcaga acagaagaac tcagcgtatt gctggcgcaa
    10201 agctacgctg ccagtggcga caacagcaat gcgctaaaag aatttaacta cgcggcagat
    10261 acttttggtt cggcagaaac acggttgcag tttgcgcttt gggcggtgag cgccaatgac
    10321 attgaaaaag ccaaaatcct acgcgtcacg ctagagaaag actggcaaca ttggaacaaa
    10381 catacacgcg gtttgtataa agcattattc aaccagctcg atgccgcgtt gtcggctaaa
    10441 taaaacgccc ttaaaaagct atttctaagg cacgtttctt cccgagaaaa aaattatgct
    10501 ctgcgagcct ttatttataa ggctcgcaga gcatcgaaaa aggttggtgt taaaaaaggc
    10561 tgttttggcc tactttttac ctaactgttt tctgcatttt tattgggcac atttggcaaa
    10621 ctgagtacat aaactaaaaa gccaatcact tgaatcgcca tcgccacaaa acccaccatt
    10681 gagagcagtt gaattttctc ttgtaacgca atggttgatt caaaccactg aaactcaatc
    10741 atgattcgaa tcacaaaaga gataagcgtt gtgatgagca agccgccaaa aaacacgccc
    10801 gcagggatcg attttgtgcg aaaaaacagc aagcctgccg caaataaaaa caaaaaactc
    10861 agcagcactt ggccatacgc agccagcact ttaaatgcgc ccaacaccat ttctagatat
    10921 agctggttag ctgacatggc aaaccctttc aaaaaagcca gttaagtgcc tgattgttag
    10981 gcgaccggca tggtctgctt aaaactggcg cgcttaacga gcacattgta atgcttggct
    11041 aaattggggt aactttcttg ccaattcaaa tgtgcaaagc gcaaatccaa atagcccagt
    11101 gcgcagccta aagcgatatc tgccaggctg aatttttcat tcacacacca ttttttcttg
    11161 gctaaatcaa ggttaagcac attgaggccg cgcgtgactt tatcaaactg cttttcaata
    11221 tgcgcgctgg attgctgggc ttctggtttg cgctgttcaa gcatcacaga aacggccgca
    11281 tcacaaacgc catcagccag cgcctcccag cggcgcactt taattttggc ggtggggtct
    11341 tgcggaatta aatgtgcgag cggcgtgcga ttgtctaaat attccacaat cacccgtgaa
    11401 tcatacaggc tttcgccatc aggcagcacc agcacgggca ctttaccaag cgggttatat
    11461 tgcttgactg ggcagtctgg gtcagccaac accacttcaa gcaggccaat ttgaatgcgc
    11521 ttttcgagtg ccacaatacg cactttgcgc gcgtacggac tattgacggt gtagaacagt
    11581 ttcatggctt gaaccttttt gtattttcac cattaaacaa caaagctgtg ctgcttaaca
    11641 atctaaagat attgataatt cgtttatttg ctgaattttg cacgttgata gcattataat
    11701 tcaaccaaca cttttaacac atataacagc accagttact tatgacactt tttagccaac
    11761 attacccgcc attttcaagt gcattttacg cgctggtgga tagccaagtg caagcggcac
    11821 ttgccgaaga tgtcggcacg ggcgacctca ctgcggcact ggtgccggcc gagcaacacg
    11881 tcactgccag cattattgcg cgcgagccag ctattatatg cgggcaagac tgggtgaatg
    11941 cctgcttttt gcaaactgat gccaacattc acattcaatg gttaattacc gaaggcgagc
    12001 atgttcaacc taagcaagtg ttgtgcaaaa taacagggcc agcccgcgca ctgttaaccg
    12061 ctgaacgctg tgctttgaat tttctgcaaa cgctttcagc cacggcaacg cataccagac
    12121 gctatgtaga tgccattacc ggcacgcaag cgcaaatatt tgacacgcgc aaaacactgc
    12181 cgcatttacg ccttgcgcaa aaatatgccg taacagtggg cggcgggcaa aatcagcgcc
    12241 tagctttata cgatggcatt ttaattaaag aaaaccatat tgccgccgct ggtggcattg
    12301 ccgaggtgat gactgcggca caggccgcga gttccggtaa gagcgtacaa attgaagtag
    12361 aaaaccttaa acagcttgaa gaagccctcg ttgcaggtgc tacttctgtc ttattagata
    12421 attttagccc tgcactgctc acagaagccg ttgcgcttaa tgtgcaatta ggtaaaaaag
    12481 cgttattaga agcttctggc ggcattacgc tagacaatgt gcgtgaaatc gcgctcacag
    12541 gggttgaccg catttcaatc ggcgccatta ccaaaaatgt cattgccatc gatttatcaa
    12601 tgcgaattca gcactaggct tgtaaatact gctttttcat gcagaataga caagtaacta
    12661 acaaaacgcg gcatattttt tgcatgcttg tttgtaatca acatgacaaa ggcaacaccc
    12721 atgaataaac ctgttgtact cagcaaccct accggccgcc tcacgctggg cgatgtgcta
    12781 cgcatgttgg taaatgacgg catggttgat aaagtacaag ctgaaaaact ttataaagac
    12841 cgtaagctag atagctcaaa actacaccca ctggtggtga taggcgaaca aaaatggaaa
    12901 aacctgctgc cgccgcacaa agcttttacg gtggaagcct tgtcccactg gctggccgcg
    12961 cgtgcaggct tagattttta tcatattgac ccgctcaaac tcgactttgg cgccgccgcc
    13021 aagttgttcg cgcaaggtta tgccgagcgt ttaaaaatta tgccgatagg tgcaaaaaat
    13081 ggtgaagcga ttatcgccac caccgaaccc ttcagcacag aatggatacc agaccttgag
    13141 cgcctgctca aaatgaaggt caaactagtg gttgccaatc cgctcgaagt cagccgctat
    13201 ttacccgaaa tttacaactt agcccactca atgagcgcgg ctgatgcctc taaagctggg
    13261 caaatcgttg gcgtacaaaa ttttgagcaa ttggttgagc ttggtaaaga caaaaacctg
    13321 gatgccaacg agcaacatgt ggtgaatatt gtcgactggt tgttcaaata tgcctttgaa
    13381 caacgcgcga gtgacatcca cctagaacca cgccgcggca tgggctttat gcgttttaga
    13441 atcgatggcg tattgcatca ggtgtatcag ttgccgccca acatcatgaa tgccatcacc
    13501 agccgcatta aactgctggg ccgcatggac atggtggaaa aacgtcgccc gcaagatggc
    13561 cgcattaaaa ccttagccgc aaatgggcaa gaaattgagc tgcgtttatc caccatgcca
    13621 accgcgtttg gtgaaaaatt agtcatgcgt attttcacac ccgaagtctc ggccagcgac
    13681 ttcttaacgc tggggttttc aaaagcgcaa gcggccacct ggaacacttg gacaaaagcg
    13741 cccaacggca ttattttagt cacaggtcca accggttcag gcaaaaccac cacgctctat
    13801 gccacgctac gccagcttgc cacgccagaa gtcaacgtgt gtacggttga agaaccgatt
    13861 gaaatggttg agccagcgtt taaccaaatg caggtgcaaa ccaatatcgg ccttaatttt
    13921 gccgactgta ttcgtacgct catgcggcaa gacccagaca ttattatg
//
LOCUS       DAOQSI010000040        13638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_3565_length_13638_cov_5.058603, whole genome shotgun sequence.
ACCESSION   DAOQSI010000040 DAOQSI010000000
VERSION     DAOQSI010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 13638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13638
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3565_length_13638_cov_5.058603"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..693
                     /locus_tag="PKL42_04160"
     CDS             22..693
                     /locus_tag="PKL42_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNU66300.1"
                     /translation="MKKFIVPLLFSIFSASASAALVTLNFEGIASNAPVNNFYNGGAG
                     GNYGVQFGSTALGIIDSDAGGTAPIANEPSPNTALAFTTLSGVSNPYLTVSTGFDTGF
                     SFFYSSSQPASIYLYDGADGTGNLLGSLTLVAQSAGNGCAGDPNGRFCNWSQVGLSFN
                     GVAKSIYFGETLNFTVFDNVTLGGNLTAVPPPVPEPETYALLLAGLGLIGFSVRRRKA
                     SQEYA"
     gene            823..1506
                     /gene="ispD"
                     /locus_tag="PKL42_04165"
     CDS             823..1506
                     /gene="ispD"
                     /locus_tag="PKL42_04165"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNU66301.1"
                     /translation="MARFHVIIPAAGSGSRMGAEAPKQYLSLNGKSLIQHVIKVFDLA
                     TRIDSIHIILSEGDAHWRSTYLHLSDKAQVHYCGGDTRAATVLNGLNAISTRVDAEDW
                     ILVHDAARPGLSNLLLNQLLNTLAHDEVGGLLALPLADTLKRADDAQRVSATIPRNDL
                     WQAQTPQMFKYATLKKALSEFVGSPTDEAEAIEALGLKPKLVTGELRNLKVTYPQDLA
                     VLSALLNQS"
     gene            1524..1997
                     /gene="ispF"
                     /locus_tag="PKL42_04170"
     CDS             1524..1997
                     /gene="ispF"
                     /locus_tag="PKL42_04170"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNU66302.1"
                     /translation="MRIGHGFDVHALVAGRKCIIGGIDIPHDKGLDGHSDADVLLHAI
                     CDALLGAAALGDIGKHFPPTDMRYKGADSRTLLRHVVALLHEQKYVVGNIDATVICEA
                     PKLSPHTAQICANIAADCRVAVTQINVKATTTEKLGFTGRREGIAAEAVCMIQAV"
     gene            1999..3825
                     /locus_tag="PKL42_04175"
     CDS             1999..3825
                     /locus_tag="PKL42_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNU66303.1"
                     /translation="MFRWFEHLVNPFPSADFNVAPKRFWPFVWACTQGMRPFIVGMTL
                     LTACIGAFEALLFAMMGKVVDRLSAVPQPLLWEYHLSDLLVLAGVLLFSTFLVGMQTL
                     LKHQTLAGNFPMRLRWNFHRLMLGQSMSFYQDEFAGRVSAKVMQTALAVRDVWFIVTD
                     IMVYVVIYFVTIVTVVGSFNLMIVWPFLLWLLFYGAALFYFIPKLARVSQQQADARAL
                     MTGRITDAYTNISTVKLFSHAGREADYAKSAMHEFLGFVNRQMRMVSGVETVNHFLNM
                     CLVIGCSGVALWLWSQGQVGVGAVAAVTAMALRLNGISHWVMWELTSLYEQVGTVQDG
                     INTLSQPHNIIDETNAQTLAVKKGEITFSKVDFAYANQKPLLAQFQLTIKAGEKVGIV
                     GRSGAGKSTIVNLLLRFYDVQSGDIAIDGQPVKQVKQNSLREHIGMVTQDTSLLHRSV
                     RDNILYGRPDATDDEMIAAAKRAEAHDFILNLRDSHGRTGYDAHVGERGVKLSGGQRQ
                     RIAIARVMLKDAPILVLDEATSALDSEVEAVIQSSLYQLMQGKTVMAIAHRLSTIAAM
                     DRLIVLDNGEIIEQGTHQELLKKHGLYASLWARQSGGFLAED"
     gene            complement(4177..4716)
                     /locus_tag="PKL42_04180"
     CDS             complement(4177..4716)
                     /locus_tag="PKL42_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNU66304.1"
                     /translation="MAKSSHENENHLFSEAVKDARPLPDHNRAFHEIKKPKPIPKQLI
                     RDEQQALVDSLSDHYIPAYEIETGEELLYLREGHSPDILRKLRRGKWVIQAHIDLHGL
                     ISDEARLYVATFIGDCKKRGIRCIRIVHGKGLGSRNREPILKHKLRGWLIQKDEVIAY
                     AQAKEQDGGSGAVVVLLKA"
     gene            complement(4823..5767)
                     /gene="trxB"
                     /locus_tag="PKL42_04185"
     CDS             complement(4823..5767)
                     /gene="trxB"
                     /locus_tag="PKL42_04185"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNU66305.1"
                     /translation="MATRHTQLLILGSGPAGYSAAVYAARANLKPVLITGIAQGGQLM
                     TTTEVDNWPADADGVQGPELMARFQKHAERFNTEMIFDHIHTTHLHEKPIRLVGDSGE
                     YTCDALIIATGASAKYLGIPSEEKFSGKGVSACATCDGFFYRNKEVAVIGGGNTAVEE
                     ALYLANIASKVTLVHRRDKFKAEAILVDKLMERVKEGKIVLETFQTLDEVLGDDSGVT
                     GMRLKNVNDGATKEIKLMGVFIAIGHKPNTDIFEGQLEMEGGYIVTQMGRAGNATATS
                     VPGVFAAGDVQDHIYRQAVTSAGTGCMAALDAERYLSQ"
     gene            complement(5925..6389)
                     /locus_tag="PKL42_04190"
     CDS             complement(5925..6389)
                     /locus_tag="PKL42_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HNU66306.1"
                     /translation="MDIGITAQDRKEIAQGLSHLLADTYTLYLKTHYFHWNVTGPMFN
                     TLHLMFEAQYNELALAVDLVAERIRALDIYAPGTYRDFAKLTSIQESETVPNAQAMIA
                     ELVAGHEAVCRTARKVFPVAETAADEATKDLLTQRLQLHEKTAWMLRSLLEK"
     gene            6505..8814
                     /locus_tag="PKL42_04195"
     CDS             6505..8814
                     /locus_tag="PKL42_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNU66307.1"
                     /translation="MFFKKSIPVNARREAEVSKSQIIGKTLIKEAWWLVLVLLGLYIT
                     VILFTYHRDDVSWVNVSANIAQIHNAGGSVGANVSALLLFLFGFSAWWWAVFAFYAMW
                     LVYLKLEVVNQADKPFLLFNLIGFALLLVASCALEAGHLISLPVSLPDARGGMIGTAV
                     DMGLRSMFGSTGTLMLLLSMFAIGFSLFTGWSWIMLTEKLGAGLIASYQFILYKWQDW
                     QDRKVGKVIEQERTEFVQTERKRSENREPVHIEKPIMEVEQSARVQKEKQAPLFESLP
                     DSVLPPLHLLDPPSGAVELPSAETLDFTSRLIERKLMDFGIEVKVLTAQPGPVITRFE
                     LEPAPGVKGSQVTNLIKDLARALSVVSVRVVETIPGKTCMGLEIPNPKRQIVHLSEIM
                     GSQAYADIHSPLAISLGKDIAGKPAVADLAKMPHVLVAGTTGSGKSVAINALILSWLY
                     KADASQVRMILIDPKMLELSVYEGIPHLLAPVVTDMRQAANALNWCVAEMERRYKLMS
                     MLGVRNLAGYNQKIRDAEKAGEKIPHPFSLTPDEPEPLTEMPLIVVVIDELADLMMVV
                     GKKVEELIARLAQKARACGIHLVLATQRPSVDVITGLIKANVPTRISFQVSSKIDSRT
                     ILDQMGAEALLGQGDMLYMPPGTGYPVRIHGAFVSDAEVHKVVNYLKAQGEPNYIEGI
                     LTNEVEEGGSDFAGGNANANSEVDPLYDEAVSIVLKTRRASISAVQRQLRIGYNRAAR
                     LIEDMERAGLVSSMQSNGNREVLAPHHEA"
     gene            8832..9458
                     /gene="lolA"
                     /locus_tag="PKL42_04200"
     CDS             8832..9458
                     /gene="lolA"
                     /locus_tag="PKL42_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HNU66308.1"
                     /translation="MYKIKTTIVSVALLLVSTSTIAMAEGIDSLKKFYNSTQSISAKF
                     HQVVTDTKGQKIQDVFGEMVIKRPNQFRWDYKKPFEQQLVSDGKQVWLYDIELAQVTT
                     RQLSKALSASPAALLAGGEDLDKNFNLSNLSREDNLDWVSALSKDADSGFNNIAIAFK
                     GDKLQQMELVDSFGHLTKIVFTDVKPNPNVSEKLFLFKAPKGVDVLVE"
     gene            9660..10994
                     /locus_tag="PKL42_04205"
     CDS             9660..10994
                     /locus_tag="PKL42_04205"
                     /inference="COORDINATES: protein motif:HMM:NF016462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNU66309.1"
                     /translation="MRFHQFNKKVQQWALCAAFLMPLCAAAMEDVVAEQARALMQSGD
                     FVGAYKLLEPLENTHAGEVDFDYLLGVAGVESGNVTRGVFALERVLATQPNHTEARTE
                     MAKAHFKLGEIESSKLEFQNVLDQKPTQEVTNAITRYVTAIDKSLGLTTTFGGFIGFG
                     VGYDSNVNSASSDNNIFIPTFGVLVANSSPEEADGFKSISGGLSVRHPINQTVSVFGS
                     ISGNSKLNNTETTFDTSSLDINAGLQIKRFIDTFTLAAQDSRFDVDSDAYRRAYGVTA
                     QWQRDFDDRNQGSLFAQATRLDYAGNAIRDADRWILGAGWAHVFSGDKSPVLFTNAYY
                     GRENARDNQADFLDQHLWGLRLGGQLSMTTKLIGYVSTSYEARHHQYKDPIFLVDKED
                     KQYEIALGLRYLPARDWVIKPELTFLKNDSNIVLNDFDRYMFSINVRKDFHW"
     gene            11099..11377
                     /locus_tag="PKL42_04210"
     CDS             11099..11377
                     /locus_tag="PKL42_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66310.1"
                     /translation="MAIKLIKLRKNNFSASATLGLLLVMTLPMLAVAEVGKVQFVTGN
                     VTAIDVNGTTRALTKGADIQNGDMIQTADGRAQVRFIDGGYMSFQPKY"
     gene            11385..12410
                     /locus_tag="PKL42_04215"
     CDS             11385..12410
                     /locus_tag="PKL42_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66311.1"
                     /translation="MEDYHFSGQADGSEKGFFRLVKGSLRAITGLIGKSNKQAWRMNT
                     PVATIGIRGTETLSEFKDGVLRVTVGDGAAYLENSQGSLILYQGQTGETDGKQPPQYS
                     AQEVKISAAAPRKAGVGSTEHQKDASTSVDEIDSNTKVAAIEKDRYFSVTDTKVDKNN
                     GSDASDTSDDIIKAIPAVILVALDKNSSSLFEGGLANLPQLHTLRAKAEYSATTPISF
                     LGGLVPSNANLTSTLKINFSNYKAEFDVQSDKFVGGLLDNRKISAEIKATLNSSTGAI
                     PFTSAPLKTTGPGANGLLTVNSAQLDPTNLSLANMSYTLQEGVTGDSAIISNQTLVGN
                     LTKKSNH"
     gene            12500..>13638
                     /locus_tag="PKL42_04220"
     CDS             12500..>13638
                     /locus_tag="PKL42_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNU66312.1"
                     /translation="MSDLFQPNTPQAPLAEKLRPKTLDEVVGQSHLLGENKPLRLAFQ
                     SGKLPSMILWGPPGVGKTTLARLIANTADAEFIPISAVLSGIKDIREAVERAELTLQQ
                     HGRKTILFVDEVHRFNKGQQDAFLPFVESGLITFIGATTENPSFEVNSALLSRSQVFV
                     LNALAESELALLLERARLLVAENITLSAPVREQVLAYADGDARRLLNFVEGLFNAAEG
                     AHISEIDEAFLQTTMAEKLRRFDKGGEAFYDQISALHKSVRGSNPDAALYWFLRMIDG
                     GADPLYLGRRIVRMAIEDIGLADPRAQGMALEACQIFERLGSPEGELALSNAVIYLAV
                     APKSNAAYNAYNQAKAFVGQDKSRPVPLHLKNAPTKLMKALDYGK"
BASE COUNT         3666 a         2792 c         3296 g         3884 t
ORIGIN      
        1 gtcttgcaaa ataggttcaa aatgaaaaaa ttcatagtac cgctgttatt ctcgattttt
       61 agtgcatctg cttcggccgc attggtgact ttaaattttg aaggcattgc gagtaacgca
      121 cccgttaata acttttataa tggcggtgca ggtggtaatt atggtgttca gtttggtagt
      181 accgcgcttg gcattattga ctctgatgcg ggtggcacag caccaatcgc taatgagcct
      241 tctcccaata cggcgcttgc ttttacaaca ttaagtggcg tcagtaatcc ttatttaact
      301 gtttcaacag gttttgatac aggattttca ttcttttata gttcatcgca accggcctct
      361 atttatcttt acgatggtgc tgatggcaca ggaaatttgc taggttcgct gacgctagtg
      421 gctcagagtg caggtaatgg ttgtgcaggt gaccctaacg gaagattttg caattggtca
      481 caggttgggc tttcatttaa tggggttgct aaatctattt attttggaga gacattaaat
      541 tttaccgtgt ttgacaatgt cactttgggg gggaatctca ccgctgtgcc gccgccagtg
      601 ccagaaccag aaacctacgc cttgctgttg gcaggtttgg gactcatcgg cttttctgtt
      661 cgacgtagaa aagcaagcca agaatacgcc tagcatcatc tgttcatatg gtgatataaa
      721 aacgggagca tctgctcccg tttttatttg tgcgaataaa aaatctattc agtggcctat
      781 gttaaaattt gcctatgcat ttcattgaat ctcaatttta atatggcaag gtttcatgtc
      841 attatccctg ctgcgggtag cggtagccgt atgggagccg aggcgcctaa gcaatacctg
      901 agtttaaatg gtaagtcgct gattcagcat gtgattaagg tgttcgatct tgcgacaaga
      961 attgatagta ttcatatcat ccttagcgaa ggtgatgcgc attggcgcag cacttatttg
     1021 catttgagtg ataaggcgca ggtgcattat tgcggtgggg atacacgtgc cgcaacggtg
     1081 ttaaatggct taaatgcgat ttcaacacgg gtagatgcag aggactggat tttagtgcac
     1141 gatgccgcac ggcctggttt aagcaatctg ttattaaatc agctattaaa taccttagcg
     1201 catgatgaag tcggtggttt attggcattg ccgctggccg ataccctcaa gcgcgcagat
     1261 gatgcacagc gcgtgtcagc tacaattccg cgtaatgact tatggcaggc gcaaacacca
     1321 caaatgttta agtatgcgac gcttaaaaaa gcgcttagcg aatttgtagg tagcccgaca
     1381 gatgaggcag aagcgattga agcgcttggc ctaaaaccaa aattggtcac aggcgagtta
     1441 cgcaacctta aagtgactta tccgcaagat ttggccgtgc tttcagcatt gcttaaccaa
     1501 agttaaagga tcaatgcatg acaatgagaa tcggtcacgg gtttgatgtg catgcgctgg
     1561 tggctgggcg caaatgcatc atcggcggta ttgatattcc acatgataaa ggtttggacg
     1621 gccattctga tgccgatgtg ctgctacatg caatttgtga tgctttattg ggcgcggctg
     1681 ccttgggtga tattggcaag cattttccgc caaccgatat gcgttataaa ggggctgatt
     1741 cacgcacatt actgcggcat gttgtggcgc tactgcatga acaaaaatac gtggtaggca
     1801 atatcgatgc cactgtaatt tgtgaggcgc caaagctttc gccacacacc gcgcaaatat
     1861 gcgcgaacat cgccgccgac tgccgcgtgg cggttacaca aatcaatgtg aaagcaacga
     1921 ccactgaaaa acttggcttt actgggcgcc gcgaaggcat tgccgctgaa gccgtatgca
     1981 tgattcaagc tgtttaatat gtttcgttgg tttgaacatt tagttaatcc ttttccgtcc
     2041 gccgatttta acgtagcgcc aaagcgtttt tggccctttg tttgggcgtg tacgcaaggc
     2101 atgcgcccct ttattgttgg catgacattg ctcacagctt gcatcggcgc ttttgaagca
     2161 ctgctgtttg ctatgatggg caaggtggtg gatagactca gtgcggttcc gcaaccattg
     2221 ttatgggaat accatctaag tgatttactc gtgcttgccg gggtactgct tttcagcact
     2281 tttctagttg gcatgcagac tttactcaag catcaaacgc tggcaggtaa tttccccatg
     2341 cgcttgcgct ggaattttca tcgactaatg cttggccaaa gcatgagttt ttatcaggat
     2401 gaatttgccg gacgtgtgtc tgcaaaggtc atgcaaacag cacttgcagt gcgtgatgtc
     2461 tggtttattg tgacggatat catggtttac gtggtgatct attttgtaac gattgtgacg
     2521 gtggttggta gttttaacct gatgattgta tggccttttt tactttggtt gctgttttac
     2581 ggtgcggctc tattttattt tattcctaaa ttagcgcgcg tttctcaaca gcaggcagat
     2641 gcccgcgctt taatgacagg gcgcattacc gatgcctata ccaatattag tactgtgaaa
     2701 ttattttcac acgcaggacg agaggccgac tacgcaaaat ctgccatgca cgaattttta
     2761 ggctttgtga atcggcaaat gcgcatggtg agtggcgttg aaacagtcaa ccatttttta
     2821 aacatgtgct tggttattgg ctgttcaggc gttgcattgt ggctttggag ccaaggacag
     2881 gtaggcgttg gcgcggtcgc ggcggttacc gccatggcct tgcggttaaa cggcatttca
     2941 cactgggtaa tgtgggaact cacctcactt tatgagcaag tgggcactgt gcaagatggc
     3001 atcaacactt tatctcaacc gcataatatt attgacgaga ccaatgcgca aactctagca
     3061 gttaaaaagg gcgagattac attttcaaaa gttgactttg cctacgcgaa ccaaaagccg
     3121 ctgttagcgc aatttcaact gaccattaaa gcaggtgaaa aagtgggcat cgtagggcgt
     3181 tcaggcgccg gaaaatcaac catcgtgaat ttactattgc gtttttatga cgtgcagtcg
     3241 ggtgatattg ccattgatgg gcagccggtg aaacaagtca agcaaaacag cttgcgtgaa
     3301 cacatcggca tggtgacgca agatacttcc ttgctgcacc gtagcgtgcg cgacaatatc
     3361 ttatacggcc gccccgatgc caccgatgac gaaatgattg ccgctgcaaa acgtgccgaa
     3421 gcgcatgatt ttattctgaa tttacgtgat tcacatggcc gtactgggta cgatgcgcac
     3481 gtaggtgagc gcggcgtaaa actctctggc ggccagcgac agcgtattgc cattgcgcgt
     3541 gtgatgttga aagacgcgcc gattttagtg ctggatgaag ccaccagtgc gctcgattca
     3601 gaagtggaag cggtcatcca aagtagtttg tatcaactca tgcaaggcaa aacggtgatg
     3661 gcgattgcgc atcggctttc taccattgct gcgatggatc ggttgattgt gttggataac
     3721 ggcgaaatta ttgagcaggg cacacatcag gaactgctga aaaaacatgg tctgtatgcc
     3781 agtttgtggg cacgccaaag tggtgggttt ttggcagaag attaagcacc attttaatga
     3841 gaaaaaacgc cgaggatttt ttaatgctct gagagccttg taaataaagg ctctcagagc
     3901 aggtgaaaag ggttgtgcta aattaaaccc agattttccg ttaagcggct cttcaagcct
     3961 acacgaatgc tggtggtcgc tttttggtta ttttcgcagc ttattcgtcg tccatagctg
     4021 gctatgaacg cctcgtaagc cacaaaacta cctcaaaaat cgcctcacta tcatttcgcg
     4081 tcctaatgga aaatcagggt taattgattt cacaaaaaaa tagtgcttaa gtcagtgctg
     4141 ttttattcaa attcaagccg cgattttgtg ggctgattaa gcttttaata acaccacaac
     4201 cgcgccactg ccgccatctt gctctttggc ttgcgcatag gcaatcactt catctttttg
     4261 tattaaccag ccacgcaatt tgtgctttaa aatcggctcg cggttgcgcg agcctaagcc
     4321 tttgccatgc acaatgcgaa tgcagcgaat gccgcgcttt ttgcagtcgc caataaatgt
     4381 ggccacataa agccgtgctt catcacttat caggccatgc aaatcaatat gtgcttgtat
     4441 tacccattta ccacggcgca gttttcttaa aatatcgggc gagtggcctt cgcgcaggta
     4501 caacagttct tcgccagttt caatttcgta cgcgggaata tagtggtcag ataacgaatc
     4561 aaccaaagct tgctgttcat cgcgaataag ctgttttgga atgggcttgg gttttttgat
     4621 ttcgtgaaaa gcgcggttat gatcaggtaa aggccgcgca tctttaactg cttcagaaaa
     4681 cagatgattt tcgttttcgt gagaagattt tgccatgact gttgcgccgc gtagtttttg
     4741 taagagcgca atttaatgcg cgaaagcaat catgatgatg aataaaagcc ttcgcgattt
     4801 aaaaaccgca cctacaacat cattattgac ttaaataacg ttcagcatct aaggccgcca
     4861 tgcagcccgt gcctgcactt gtgaccgctt ggcgatagat gtgatcttgc acgtcccctg
     4921 ccgcaaaaac acccggtaca cttgttgctg tggcgttgcc tgcgcggccc atttgcgtca
     4981 cgatatagcc gccttccatt tcaagctggc cttcaaatat atcagtatta ggtttgtggc
     5041 caatggcaat aaatacgccc atgagcttga tttctttggt tgcgccgtca ttcacatttt
     5101 ttaaacgcat gccagtcaca ccgctgtcat cgcctaacac ttcatctagc gtttgaaagg
     5161 tttctaagac gattttgcct tctttgacgc gctccattag cttgtcaacc aaaatggctt
     5221 cagctttaaa tttgtcgcga cggtgtacta atgtcacctt gctggcaatg ttagcaaggt
     5281 agagcgcttc ttcaacggcg gtgttgccgc cgccaatcac tgcaacttct ttattgcgat
     5341 agaaaaaacc atcgcaggtg gcgcaggcag aaacgccttt tcctgaaaac ttctcttctg
     5401 acggaatgcc caaatatttt gccgacgcac ctgttgcaat aatcaacgca tcgcaagtgt
     5461 actcaccgct gtcgcccact aagcgaatgg gtttttcatg tagatgcgtg gtatgaatat
     5521 ggtcaaaaat catttcggta ttaaagcgtt cagcatgttt ttgaaaacgc gccattaatt
     5581 ctggcccttg cacaccgtca gcgtctgcgg gccagttatc aacttcggta gtcgtcatca
     5641 gttggccgcc ttgtgcaatg cccgtgatga gcactggctt aaggttggcg cgcgcggcat
     5701 acaccgcagc agagtagccc gcggggcctg agcctaaaat aagtaattga gtatgacgtg
     5761 ttgccatgtt aagcgacttt ctatatttaa aaatttgtgt tgaatatctg caagcagttg
     5821 aggccgagcg cgttactttt caagcagact tcttagcatc catgcggttt tttggcttcg
     5881 gcataaccta aaggttagcc tacaccgaaa gacagcctat ttacctactt ttctaataag
     5941 cttcttagca tccaggctgt cttttcatgt aattgcaaac gttgtgtgag tagatctttt
     6001 gtggcttcat ccgcagccgt ttcagccaca ggaaaaacct tacgcgccgt gcggcatacc
     6061 gcttcatggc cagccactaa ttcagcaatc atcgcttgcg cgtttggcac agtttccgac
     6121 tcttgaatcg acgttaattt ggcaaaatcg cgataagtac ctggtgcgta aatgtctagg
     6181 gcgcgaatgc gttcagcaac taaatccacc gcaagcgcca attcattata ttgggcttca
     6241 aacatgagat gtaaggtatt aaacatcggc cctgtgacat tccagtgaaa ataatgcgtt
     6301 tttaaataga gcgtgtaagt atcagccaaa agatgcgata agccttgcgc aatctctttt
     6361 ctatcttgtg ctgtaatgcc aatatccatg atgaactcct atgaaaaatc acgattaatt
     6421 ctagtatgct attgtattag ctacagagcg actttgatac tgctataatt tgttttaatg
     6481 ttaaatttgt aaggttttaa tatcttgttt ttcaaaaaat caatcccagt taacgcacgt
     6541 cgcgaagcgg aagtcagcaa gtcgcagatc atcggcaaaa ccctgattaa agaagcttgg
     6601 tggctggtgt tagtgctgct cgggctctat attacggtca ttcttttcac ctatcatcga
     6661 gatgatgttt cgtgggtcaa tgtgtcggca aatattgcgc aaattcataa tgcgggcggt
     6721 agcgtgggcg ccaatgtttc ggctttactg ctgtttttat ttggcttttc tgcttggtgg
     6781 tgggcggtct ttgcttttta cgccatgtgg ctggtttatt taaaactaga ggtggtcaat
     6841 caagccgata aaccattttt attgttcaac ctgattggtt ttgccttgtt gttagtggct
     6901 tcttgcgcct tagaggcagg gcatcttatc agcttgccag tgtcattacc tgacgcacgc
     6961 ggcggcatga taggcacagc ggttgatatg ggtctacgta gcatgtttgg cagcacaggc
     7021 acattgatgt tgctgctttc aatgtttgcc attggtttta gtctgtttac gggctggtct
     7081 tggattatgc tgactgaaaa actgggtgct ggcttgattg cgagctatca gtttatccta
     7141 tacaaatggc aagattggca agatagaaaa gttggcaaag tgattgagca agaacgtact
     7201 gaatttgtgc aaactgagcg taaacgttct gaaaaccgtg aaccagtgca tattgaaaaa
     7261 ccaataatgg aagttgagca aagcgcgcgt gttcaaaaag aaaaacaggc gccgttattt
     7321 gaatcattac cagactcagt attgccacca ttacatttat tagatccgcc atcgggtgct
     7381 gttgagttac cttctgctga aacgctagac ttcacttcac gtttgattga acgcaaactg
     7441 atggattttg gcattgaggt gaaagtgtta accgcacaac caggaccagt gattacccgt
     7501 tttgaattag aaccagcacc tggcgtaaag ggcagccaag tcactaattt aattaaagat
     7561 ttagcgcgcg cgttatccgt agtaagtgtg cgtgtggttg aaactattcc cggtaaaact
     7621 tgcatggggt tggaaattcc aaaccccaaa cgtcaaatcg tgcatttatc cgaaattatg
     7681 ggtagccaag cttatgctga cattcattcc ccacttgcga tttcccttgg caaagacatc
     7741 gcaggtaaac cggcagtggc tgatttagcc aaaatgccgc acgtgttggt ggcgggtacc
     7801 actggttctg gtaaatctgt tgccattaac gcgctgattt tgagttggct ttataaggcc
     7861 gatgccagtc aagtacggat gattttaatt gacccaaaaa tgctagagct ttcagtctat
     7921 gaaggtattc cacatttatt agcacctgtt gtcaccgata tgcgccaagc tgcaaatgcg
     7981 cttaactggt gcgtggctga aatggaacgt cgctataaat tgatgtcgat gttaggggtg
     8041 cgtaacttag ctggctataa ccaaaaaatc agagatgctg aaaaagccgg agaaaaaatt
     8101 ccacatccat tcagcttaac accagatgaa cctgagccac tgacggaaat gccgctgatt
     8161 gttgtggtga ttgatgagtt ggccgattta atgatggtag tgggtaaaaa agttgaggaa
     8221 ctcattgcac gtttagcgca aaaggcgcgt gcttgtggaa ttcatctcgt cttggcaaca
     8281 cagcgcccat ccgtcgatgt gattacaggg ttgattaaag ccaatgtgcc aacgcgcatt
     8341 tcttttcagg tgtccagcaa aattgattca cgcaccatat tagaccaaat gggcgctgaa
     8401 gcgttattag ggcagggcga tatgctctac atgccgcccg gtaccggtta ccccgttcgc
     8461 attcatgggg cttttgtttc tgacgctgaa gtgcataagg ttgtgaatta cctcaaagca
     8521 cagggtgaac caaattatat tgaaggtatc ttaaccaacg aggttgaaga aggtggtagt
     8581 gatttcgcag gtggtaacgc aaacgcaaat tcagaagtgg accctttgta tgatgaagcg
     8641 gtgagtattg tgcttaaaac gcgtcgtgca agtatctctg ctgtccaaag gcaactgcgc
     8701 atcggctaca accgtgcggc ccgtttgatt gaagatatgg agcgagcagg gttggtgtct
     8761 agcatgcaaa gtaacggtaa ccgcgaagtg ctggcaccgc accatgaagc gtaaccttat
     8821 aaaggaatta aatgtataaa attaaaacta cgattgtttc agttgcgttg ctactggtaa
     8881 gtacatcaac catcgcaatg gctgaaggca tcgacagcct aaaaaaattc tataacagca
     8941 cacaatctat tagcgcaaag tttcaccaag tagtgactga taccaaaggt cagaaaattc
     9001 aagatgtatt tggtgaaatg gtgattaagc gacctaatca atttcgatgg gactataaaa
     9061 aaccttttga acaacaattg gtgagcgatg gcaagcaagt gtggctctac gatattgaat
     9121 tggcacaagt gaccacccgt caattaagca aggcgttaag tgctagtcct gctgcattat
     9181 tagcaggggg tgaagacctt gacaaaaatt ttaacttatc taatttaagt cgtgaagata
     9241 acctcgactg ggtgtcagcc ttgtctaaag atgctgattc aggttttaac aatattgcca
     9301 ttgccttcaa gggtgataaa ttgcaacaaa tggagcttgt agatagtttt gggcacctta
     9361 cgaaaattgt gtttaccgat gtgaaaccga accccaatgt tagtgaaaaa ctctttttgt
     9421 ttaaggcacc taagggagtg gatgttttgg ttgagtaggt acatctaact ttgcttaaaa
     9481 ctatattttt taatagtatt taaactgaaa tggcgctaaa tgattggcgc catttttttg
     9541 ctaaaaaaca acactcattt atgaatgacc acgtattata taaaatacta gcaaaaaaat
     9601 aattcatcat tttttcaata agttgtatag tgttcttaat ttatttggtt aggctttgca
     9661 tgcgatttca tcaattcaat aaaaaggttc agcaatgggc actttgtgca gcatttttga
     9721 tgcccctttg tgctgcggcg atggaggatg tcgtggcaga gcaggcgcgc gccttgatgc
     9781 agtcaggaga ttttgtaggg gcatacaagc tacttgaacc attggagaac acgcacgcag
     9841 gtgaagtgga ttttgattat ttgctgggcg ttgccggggt tgaaagcggc aacgtgacac
     9901 gcggtgtgtt tgccctagag cgcgttttgg ctacacagcc aaatcatact gaagccagaa
     9961 cagaaatggc aaaagcccac tttaaactgg gtgaaatcga gtcttctaag cttgagtttc
    10021 aaaatgtgct tgatcaaaaa cccacacagg aagtgactaa tgcaattacg cgttatgtga
    10081 ctgccattga taagtccctt gggctaacga ccacttttgg tggttttatt gggtttggtg
    10141 ttggttacga tagcaatgta aacagtgcat catcagataa caatattttt attccaacct
    10201 ttggtgtatt ggtagcaaat tcgtcgcctg aagaggctga tggttttaaa agcatttctg
    10261 gtggtttgtc tgtgcggcac cctattaacc agacagtttc ggtatttgga agtataagcg
    10321 gtaacagtaa gctcaataat acagagacta cttttgatac cagtagctta gatatcaatg
    10381 caggtctaca aattaaacga tttatcgata cgtttacatt ggcggcacaa gatagtcgat
    10441 ttgatgtaga tagtgatgct tatcgacgtg cctacggcgt aacggcgcag tggcaacgcg
    10501 attttgatga ccgtaaccaa ggtagccttt ttgcgcaggc aacgcgctta gattatgcag
    10561 gcaatgctat tagagatgca gaccgctgga ttttgggtgc gggttgggca catgtttttt
    10621 cgggtgataa aagcccagtg ctttttacca atgcttatta tggtcgggaa aatgcccgtg
    10681 acaaccaagc agatttttta gaccagcatt tatggggttt acgtttaggt ggacaattgt
    10741 caatgaccac aaaactcatt ggctatgtct caacaagtta tgaagcgcgt caccatcaat
    10801 ataaagatcc tatattttta gtcgataaag aagacaagca atatgaaatt gccttgggat
    10861 tacgctattt gccagcgcgt gactgggtaa ttaaacccga attgactttt ttaaaaaatg
    10921 attcaaatat cgtattgaat gattttgatc gctatatgtt ttcaatcaac gtacggaaag
    10981 attttcattg gtagtcttta ggaatgaaaa ttgcatgagt atttgcttca attgcgcagt
    11041 tgttaagaaa tggctcatct aaatgtgggt aatttcagca aaaaaacggg aataatttat
    11101 ggctatcaaa ttgattaaat tgagaaaaaa taattttagt gctagtgcaa cactcggact
    11161 gttgctggtg atgacgctcc ctatgttagc agttgcagaa gtcggcaagg tacagtttgt
    11221 gacaggaaat gtcactgcca tagacgtaaa tggtacaact agggcactga cgaagggtgc
    11281 ggatattcaa aatggcgaca tgatacaaac cgctgacgga cgtgcgcaag tacggtttat
    11341 cgacggtggc tatatgtcat ttcaacctaa atactgagtt taaggtggaa gactatcatt
    11401 tttcaggtca agctgacggg tctgaaaaag gtttttttag gttggtaaaa ggtagcttaa
    11461 gggcgattac tggtttaatt ggaaaatcta ataagcaagc gtggcgcatg aataccccag
    11521 tggcaacgat tggtattcgt ggtactgaaa cattaagtga atttaaagat ggcgtgctgc
    11581 gtgtgacggt aggcgatggt gcagcctatc ttgaaaatag ccaaggtagc ttaattttat
    11641 atcaaggcca aacaggtgaa accgatggta agcagccacc gcaatatagt gcacaagaag
    11701 ttaaaatcag tgctgcagca cccagaaaag caggggtagg cagtaccgaa catcaaaaag
    11761 atgctagcac gtcagtggat gaaattgaca gtaatactaa agttgctgcc atagaaaaag
    11821 acagatattt ttctgttact gatactaaag tagacaaaaa taatggcagt gatgcaagtg
    11881 atactagtga cgatattatt aaagcgattc cggctgttat tttggttgcg cttgataaaa
    11941 attctagtag tctgtttgaa ggggggttgg caaatttacc gcaattgcat acgctgcgtg
    12001 ctaaagcaga atatagcgcg accacaccca ttagctttct tggtgggctg gtgccgtcca
    12061 atgccaattt gacgtcaacc ttgaaaatta acttttctaa ttataaagca gagttcgacg
    12121 tgcaatctga taagtttgtc ggcggcttat tggataatcg taaaatttca gctgagataa
    12181 aggcgacgct aaattcaagc acaggggcga ttccatttac cagcgctccg ttaaaaacaa
    12241 ccgggccagg tgccaatggc ttactgactg tgaattccgc ccagctagac ccgacgaatt
    12301 tgtcactggc caatatgtca tatacgctac aagaaggtgt cacgggtgat agtgccatta
    12361 tttctaatca aacgttagtg ggtaatctca caaaaaaatc taatcattga aaatattggg
    12421 tgaatgttta tcattgcaac ttgacgtaat atgtcaatga gtccatttgc tatgctaaac
    12481 actagaaaaa acccatattt tgagcgattt atttcaacct aataccccgc aggcaccttt
    12541 agctgaaaag ttacgtccca aaactttaga tgaagtggtg gggcaatcac atttgctggg
    12601 tgaaaataag cctttaaggc tagcatttca atcaggcaaa ttgccctcta tgatattgtg
    12661 gggcccgcca ggtgtgggta aaaccacatt ggcgcgactc attgccaata ctgctgacgc
    12721 tgaatttatt cctatttctg ctgtgttatc gggtattaaa gacattcgtg aggcagtaga
    12781 gcgggcagaa cttacactac agcaacatgg ccgcaaaacc attttgtttg tggacgaagt
    12841 gcatcgcttt aataaaggtc agcaagatgc ttttttgccg tttgtggaaa gtgggctgat
    12901 tacctttatt ggcgccacca ctgaaaaccc atcgtttgaa gtcaacagcg cgctattaag
    12961 ccgttcgcag gtatttgtgc tgaatgcgct tgctgaatca gagttggcat tgctattaga
    13021 acgagccaga ttattagtag ccgaaaatat tacgctctct gcaccggtta gagagcaggt
    13081 attagcgtat gctgatggtg atgcaagacg cttgcttaac tttgttgaag gtttatttaa
    13141 tgcggctgaa ggcgcgcata tttcagagat tgatgaagcc tttttacaaa ccaccatggc
    13201 agaaaaactg cgccgttttg ataaaggcgg cgaggcgttt tacgaccaaa tttcggcctt
    13261 gcataaatct gtgcgcggtt caaacccaga tgcagcgtta tactggtttt tacgcatgat
    13321 agatggtggc gctgacccgc tgtatttagg ccggcgtatt gtgcgcatgg cgattgaaga
    13381 tattggcttg gcagacccac gcgcgcaagg gatggcactg gaagcctgcc agatttttga
    13441 gcgcttaggt tcgccagaag gagaattagc gctatcaaac gcagtgattt atttggcggt
    13501 tgcgcccaaa agtaacgcgg cctataacgc gtataatcag gccaaagcct ttgttggcca
    13561 agataaatcg cgccccgtgc cattacattt aaaaaacgcg ccaactaaat tgatgaaggc
    13621 gttagattac ggcaaaga
//
LOCUS       DAOQSI010000041        13328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_3701_length_13328_cov_5.400211, whole genome shotgun sequence.
ACCESSION   DAOQSI010000041 DAOQSI010000000
VERSION     DAOQSI010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 13328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13328
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3701_length_13328_cov_5.400211"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1276
                     /locus_tag="PKL42_04225"
     CDS             <1..1276
                     /locus_tag="PKL42_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNU66313.1"
                     /translation="KSFEDSQFQQFLIAQGYAAERLPIKLWHLDDLTYCALFFHPSLE
                     VARAQWRTAQLAEQVAAQKPLPTLSSHLAHSDDPDPEKKPFALGLSIDIPIEVANKRQ
                     VRIEQAQHLSQIAKLEIAQTAWQLRHQLAQTLSSLQYNQQQRTVLTQEKALREAIVGL
                     YQKRLSLGEASNIELSNATLQLQTSANALLSAQQAQLVLQSQLASHLGLPLAQVNKMM
                     LAPMDDHRQLSAIPTDEIQATALLNRIDIRMALERYAVAEAKLKLEIANQYPDLVISP
                     GYTYEFGDNVWSLGISSLLKLLNKNKTNIASASQLREVEAAQFEALQTKVIQDAATAH
                     AQLTQANAELQDQQRLLMQQQANTQRIARQFSAGEVDRLAITYAELENVGAEKAVALS
                     RYKRNQALDQFENAMQKPIATQSLFNQYRGEL"
     gene            1273..2358
                     /locus_tag="PKL42_04230"
     CDS             1273..2358
                     /locus_tag="PKL42_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66314.1"
                     /translation="MSRKAMMIIGIQAFLIIVLFWLLVFYGKDEYEELGAELDEEVET
                     RSLVTEHDSNDAGVATLVLPVALQKQSGLQFTRLQATTHQASSAAFGTVESIDSLVEL
                     RTRYFAALADGNVIRSNIASAEQKLQRLKLLNQDDKNVSDRVVQEAEALLNSEKAKLG
                     ASSTSANGIRDNIRQLWGSTLANWATQNTPNNTFQGLLQHRDVLLKVTLPFDVVPDKN
                     TMLNITPMGAVSQFIQAKFISEAALSDSTVQGKTYYYHAPAGNLRTNMRVSARLEKQD
                     KVTTGVIVPHQAVVWYANQAWVYQKTGAEKFVRRLINTDIEIESEAISGWYNTAEVAE
                     NDEVVVSGAQLLLSEELKYQIQNENED"
     gene            2467..5598
                     /locus_tag="PKL42_04235"
     CDS             2467..5598
                     /locus_tag="PKL42_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNU66315.1"
                     /translation="MMSALVRFSIRFHGVVIGLACLLVVYGLYNLSRANLDVFPEFSP
                     TQVVIQTESPGLSSSLVEQLVTQPIENTISGTVGVESMRSQSIPGLSIITIIFNENTD
                     IYKNRQVVAERLATLSNQLPKNIVPNITPLTSSASSVLGMGITSDKRSLMELRTLVDW
                     TIRPHIMAVPGVADVNVLGGDVRQFQILVDPKKLIQYNLSIQEVLAVTARVTGVRGAG
                     YIENNNQRILINTEGQARNANELAMMPILHRQGKTVRLSDIGEVVVGAAPSISAAAIN
                     GKEGVYFSVQGQLGANTKAVTEAVEKAMQELEPELKAQQITFYPELFRPANFIETAID
                     GVKTDILIGSVLVIAILFLFLFNARTAFISATAIPLSLLAGTIVLSYYGIGLNIMVLG
                     GLAIALGEVVDDAIIDTENIFRRLRENRLLTNPLPAHQVVFNASMEVRSSVVYATIIV
                     VLVFLPLLTLSGVAGKLFAPLGYAYISAIVASLVVALTLTPALCYLLFTHGKLESGDP
                     PLIAWLKVRYERLLKQIEKRHIMILSVSTIIIALGLGILPLFKSQFIPSLHEGHYIMH
                     MTAVPGTSAQESLRIGKRVSKVIENIKGVKSVTQWVGRAPNAADTFGTHYSEFEIELG
                     NASGPEQRYILNRIREELAGEVEDEDGDGIAEIGFIGVNFAINTFLTERIEETISGYT
                     AAMVINIHGQDLDALDRDAREVAELLSSISGAADIQIQSPPGTPELTIRLRPERLAMY
                     GLQSLDVLENIQAAYEGVQATQIYQGNQVTSVNVMFTHNVRDDLREIGLLPLKNPDGK
                     MILLKEVANITQENGRSKILHAGAKRIQTVTCNIQDRSVTSFADELKKRINKEAKLTP
                     GNFVTYTGEAEANAQSREDLIVHSLLAGVGIFLMLYIAFGRLRNLLLTFANLPFALIG
                     GVIAAMFTGGWISLGSLVGFVTLFGITLRNSIMMVSHYQHLVDEEHCVWGLETCIRGA
                     SERLPSILMTALVTALGLLPLAMGSGQPGREIEGPMATIIVGGLVTSTILNLLILPTI
                     MLHFGKFEKPMQV"
     gene            complement(5790..6167)
                     /locus_tag="PKL42_04240"
     CDS             complement(5790..6167)
                     /locus_tag="PKL42_04240"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNU66316.1"
                     /translation="MTKIKAYTDDEVQQKLAKELPHWFLENGWIRRKYKTSGWKATLM
                     VVNTIGHLAEAAWHHPDLTVSYAFVIVKLCTHDAKGITDKDFELAHKIEQVIGWQPAL
                     EGGVLTGTPNDDVRFKYIKYDEV"
     gene            complement(6180..6818)
                     /locus_tag="PKL42_04245"
     CDS             complement(6180..6818)
                     /locus_tag="PKL42_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridylate kinase"
                     /protein_id="HNU66317.1"
                     /translation="MWVVKLGGSLLGAPELANWLETLVKFSDGKVVIVPGGGLFANAV
                     REAQQIANVSDALAHQLALLAMDQYGLLLTGLNPALVTARSELEISERGWQHRAIVWL
                     PSKMVLADDTIPKNWEITSDSLSAWLANKLGAEQLILVKSKALLAYQTNAPTRLQHLV
                     DDELIDKQFINYIHGQSYQTWVVNKADYAIFAQGVNVDALQAKGLQLSTSVV"
     gene            complement(6841..8262)
                     /gene="pabB"
                     /locus_tag="PKL42_04250"
     CDS             complement(6841..8262)
                     /gene="pabB"
                     /locus_tag="PKL42_04250"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HNU66318.1"
                     /translation="MLKKALPYHHDSAKLFECIAHLDWPMMLDSGQALNPQTGEPGSQ
                     YGRYDILVASPVKTFVTYGKRTEITENGLTEVSEENPFELIKSALAQYISSENASTDS
                     PFSGGAVGYFGYDLALYFEKLPSVDIQGTPKSMDAIPELMVGIYDWAVVVDHRQKTAH
                     LIAHFCHENAREKFNALEALFYKASSGAKKVEAFKVTTDVISNMTKARYAHAFDKVKN
                     YILEGDCYQVNLAQRFSAQAKGDGWLAYKKLREISPAPFMAYMQLPHLEVLSASPERF
                     LQVVGQHVETRPIKGTRPRSDDAIEDAKQAIELQNSLKDRAENLMIVDLLRNDIGKNC
                     EIGSVKAEKLFQLQSFANVHHLVSIVTGKLAQGKSALDLLRGCFPGGSITGAPKLRAM
                     QIIDELEPSPRGLYCGAIGYIGFDGNMDTNIAIRTAVYTQNEVSFYAGGGIVADSTME
                     KEYIETLDKASSMLRLMQFFKVD"
     gene            complement(8256..9293)
                     /locus_tag="PKL42_04255"
     CDS             complement(8256..9293)
                     /locus_tag="PKL42_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HNU66319.1"
                     /translation="MVKNNSVIGWDVGGAHLKAVLRDATGKVLAAKQVYCPLWRGLNE
                     LATAIDDVLLEMQATQHAVTMTGELADIFSNRQAGVVQIAQLAAKKLTGEVRFFAGNA
                     GFVPLNLVEHHAHDIASMNWLASLQYLAQQVKQALFVDIGSTTTDLAILHSHAPQVLG
                     FNDAARLKTDELVYTGVVRTPLMALAQKIPFNGDWVNVAAEHFATTADVYTLTGELPL
                     HDNMSDTSDHAEKSVEASARRIARMVGMDATDAPLSAWRQLAHSFKTAQLNQLQLAML
                     RQISRLQDQQSLQVIGCGAGEFLAAELAQRLGLQYVSVTHYIAAENEELTSSAAVCFP
                     AFAVAGLGLAC"
     gene            complement(9287..10333)
                     /locus_tag="PKL42_04260"
     CDS             complement(9287..10333)
                     /locus_tag="PKL42_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNU66320.1"
                     /translation="MKEKQEKLFVCEFITGGGLSDEALPESLVHEGTLMRDALLRDLN
                     QMKQYALITTHDARLPQPALARKSLPVIASDFKKTFKKALKQADLVWVIAPETDGALL
                     GLTELCLAEEDKEHGAQFLGCGFDATLIGTSKSLTFDTLQAAGIHTLPVYSGEDLIQP
                     AFFEEMSTLKVAKWLAKPEDGAGCEGIRLFDSLTALRDWLQEDQRYLLYFAQPYQSGI
                     NASFAYLSGGGGLAWLLCCNEQHICQTGQLFKLTGITVNGMVAYWQRFETLMRKLAKE
                     LPDALGYVGVDVIIDTQANKIYVIDINPRLTTSYVALTEAIGCNAAKLILDNQCDESF
                     KMPRIQKNIVEIQW"
     gene            10344..11048
                     /locus_tag="PKL42_04265"
     CDS             10344..11048
                     /locus_tag="PKL42_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HNU66321.1"
                     /translation="MQIIPVIDLLNGVVVHAKRGERQQYQPIQSKLCDSHAPLAIVSA
                     LLDNMPFTQLYIADLNAIQKLEAQYTTNYSAIAAIAKKYPQITLWVDAGISNNTELQY
                     WQKLKVRLVIASENFKTIAQYHSLNLAETPHILSLDFFPQGYAGPLELLANCQHWPQD
                     VIVMSLENVGANAGVNTALLMEIKARAQQHSLIAAGGIRNIDDLRALEALGIQQALMA
                     TALHQQQITAAQLAKL"
     gene            complement(11142..11681)
                     /gene="fae"
                     /locus_tag="PKL42_04270"
     CDS             complement(11142..11681)
                     /gene="fae"
                     /locus_tag="PKL42_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HNU66322.1"
                     /translation="MAVVDRVLVGEALVIENRPNLENCPDLRNVAHIDLIMGPRGSTA
                     EEAFCRTLTDQKVGVNGLLALIAPNLPCKPATVMFNKVTIKNEKQAVQMFGPAQHGIA
                     MAVMDCVVDGTIPKNEVDDIFICVGVYISARADMDDRVQEWNYLAMKQAIKNAVNREP
                     NLETIIEKYQETRHPFQAK"
     gene            complement(12012..12551)
                     /locus_tag="PKL42_04275"
     CDS             complement(12012..12551)
                     /locus_tag="PKL42_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNU66323.1"
                     /translation="MSSSQKLILDKLTSILAETDNAKSAELLKLVEGAGRTFIGGAGR
                     SLLVSRFFAMRLVHAGYNVSMVGEVVTPAIKAGDLLILVSGSGGTETLLPFVKKAKSV
                     GAKLAVISMKKSSAMADVADLVIQIGNDNSFPLTKGMPMGSQFELSTLIFLEAAIADL
                     IFAKDLTEEGMRAIHANLE"
     gene            complement(12671..13303)
                     /locus_tag="PKL42_04280"
     CDS             complement(12671..13303)
                     /locus_tag="PKL42_04280"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HNU66324.1"
                     /translation="MALTQMALDSLDFDGTVALATLVAPHVDILEIGTPCIKHNGIKL
                     LEVLRAKFPNNKILVDLKTADAGFYEAEPFFKAGADIVTVLGISDIGTIKGVIDVANK
                     YGKKAQVDLINVADKAAVTKTVAAAGAHIIGVHTGLDQQAAGQTPFADLALVAGLNLG
                     VEISVAGGVKAATAAQVRDAGATIIVAGAAIYGAADPAAAAAEITKIAHG"
BASE COUNT         3665 a         3176 c         2883 g         3604 t
ORIGIN      
        1 caaatcattt gaagacagtc agtttcaaca gtttttaatc gcgcaaggtt atgcggctga
       61 gcgcttaccg attaagcttt ggcatttaga tgacttaacc tattgcgcat tgttttttca
      121 tcccagttta gaggttgccc gagcacagtg gcgcacagcg cagcttgcag aacaagttgc
      181 agcacaaaag ccgctcccta ctttaagcag ccatttggcg catagcgatg acccagaccc
      241 agaaaaaaaa ccttttgcgc tcggcttgag tatagatatt ccgatagaag tcgccaataa
      301 acgtcaggtg cgtattgaac aagcacagca tttatcgcaa attgccaaac ttgaaattgc
      361 acaaactgcc tggcaattac gtcaccagct cgcacaaacg ctgtcaagtc ttcaatataa
      421 tcaacaacaa cgcacggtgt taacgcaaga aaaagcgctg cgcgaagcaa ttgtgggtct
      481 ctaccaaaaa cgactcagtt tgggcgaagc ctctaatatc gaactaagca atgcaacact
      541 acaactgcaa accagtgcaa atgcattgct aagtgcgcag caagcgcaac tggttttaca
      601 atcacaactc gctagccatt tagggcttcc gttggcacaa gtcaacaaaa tgatgttggc
      661 accgatggat gaccatcgtc aattgagcgc aatccctact gatgaaattc aggcaactgc
      721 cctgctcaac cgcattgaca tccgcatggc gcttgaacga tatgcggtgg ctgaggctaa
      781 gttaaagcta gagattgcca accaataccc tgaccttgtt attagccctg gttacactta
      841 tgaatttggt gacaacgtgt ggtcattagg catttcaagt ttactgaaac ttcttaataa
      901 aaataaaacc aatattgcgt cagctagcca actacgagaa gtggaagctg cacaatttga
      961 agcattgcaa acaaaagtga ttcaagatgc cgctacggcc catgcgcaac tcacgcaagc
     1021 gaatgctgaa ctacaagatc agcaacgatt attaatgcaa cagcaggcaa acacacaacg
     1081 tatagcacgc caattttctg cgggtgaagt tgatcggctc gccattacat atgccgaatt
     1141 agagaatgtt ggggctgaga aagcggttgc attgtcccgc tataaacgca atcaggcact
     1201 agaccaattt gaaaacgcca tgcaaaaacc aatcgcaaca caaagtttgt ttaatcagta
     1261 tcggggtgaa ctatgagccg taaagcaatg atgattatcg gcatacaagc atttttaatt
     1321 attgtacttt tttggctatt ggtgttttac ggtaaagatg aatacgaaga gcttggtgca
     1381 gaactagatg aagaagttga aactcgctca ctagtgaccg agcacgatag taatgacgcc
     1441 ggtgtcgcaa ccctagtgct gccggttgca ctgcaaaaac agagtggttt gcaattcaca
     1501 cggttacagg caaccacgca tcaagcttca tcggcggcgt ttggtactgt ggaatccatc
     1561 gacagcttag ttgagcttcg tacccgctat tttgcagcac ttgctgatgg caatgtgatt
     1621 cgtagcaata ttgcgagtgc tgaacagaag ctgcaacgct taaagctgct caaccaagac
     1681 gataaaaacg tttctgaccg tgtggtgcaa gaagcggaag ccctgctcaa tagtgaaaaa
     1741 gcaaaattag gcgcttccag cacgtcagcc aatggcatac gcgataatat tcggcaactt
     1801 tggggttcaa ctctggctaa ctgggctaca caaaacacgc caaataatac atttcagggc
     1861 ctattgcaac accgtgatgt gctactgaaa gtgaccctgc catttgatgt ggtgcctgat
     1921 aaaaacacca tgctgaacat cacaccgatg ggtgcggtca gtcaattcat acaggcaaag
     1981 tttatttctg aagcagcact ttctgatagc acagttcaag gtaaaactta ttattaccac
     2041 gcgcctgctg gtaacttaag aaccaacatg cgtgtttcag caaggctgga aaaacaagac
     2101 aaagtaacga ccggcgtgat tgttccgcat caagccgtgg tttggtacgc aaaccaggct
     2161 tgggtatatc aaaaaacagg ggcagaaaaa tttgttcgcc gtttgattaa cactgacatt
     2221 gaaattgaaa gtgaagcgat tagtggctgg tataacaccg cagaggtcgc agaaaatgat
     2281 gaagtcgttg tcagcggtgc acagttattg ctgtctgaag aattaaaata ccaaattcaa
     2341 aacgaaaacg aggattagat tgttaaattt atacttcgtt acaatgaata cgcacgtagc
     2401 aacgcatcgt tttatcgtcg cgctacaaag tgtaatgatc attagcacac aaaggcagcc
     2461 gttgccatga tgtccgcgtt ggttcgattt tcaatacgct ttcacggtgt cgtcattggc
     2521 ctggcatgcc tgttggtggt ctatggtttg tataacttat ctcgcgcaaa tctggatgtg
     2581 tttccagaat tttcgcccac gcaagttgtt attcaaactg aatcacctgg tttgtcgtca
     2641 agcttagttg agcaactggt aacacaaccg attgaaaata cgatttccgg tacggttggc
     2701 gttgagtcga tgcgctcaca atcgattccc ggtttatcca tcatcacgat catttttaat
     2761 gaaaacaccg atatttataa aaatcgtcaa gttgttgctg aacgcttagc aactcttagc
     2821 aatcaattac ctaaaaatat tgtacctaat atcacgccgc tgacttcaag cgccagttcg
     2881 gtgctaggca tgggcattac ttcagataaa cgtagcttga tggaactacg caccttggtg
     2941 gactggacga tacgcccaca tattatggca gtgccgggtg tggcagatgt aaatgttttg
     3001 ggcggtgacg tgcgccagtt ccaaattcta gtggacccta aaaaactcat tcagtacaac
     3061 ttatcaattc aagaagtctt ggctgtcact gcgcgcgtta ctggcgtgcg cggtgcagga
     3121 tatattgaaa ataacaatca gcgcatttta attaacacag aaggtcaggc cagaaacgca
     3181 aacgaattgg ccatgatgcc aattttacat cgccaaggta aaaccgtgcg tttaagtgat
     3241 attggtgaag ttgtggtcgg tgcagctccc tctatcagtg cggcggccat caatggcaag
     3301 gaaggggtgt atttttcagt acaaggccaa ttgggggcca ataccaaagc cgtgactgaa
     3361 gcggttgaaa aagccatgca agaactcgaa cccgagttaa aagcacagca gattacgttt
     3421 taccctgagc tattccgccc agcaaatttt attgaaacgg ccattgacgg cgttaaaaca
     3481 gacattttaa tcggctctgt gctggtcatc gccattttgt ttttattttt atttaatgct
     3541 cgtaccgcat ttatctctgc cacggccatt ccactttcat tgctggcagg cacgattgtg
     3601 ctgagttatt acggcattgg gcttaacatc atggtgctag gcggcttggc gattgcgctg
     3661 ggtgaagtgg ttgacgatgc cattatagac acagaaaaca ttttcagacg gctacgtgaa
     3721 aaccgcttac tgaccaatcc cttgcctgct caccaagttg tttttaatgc atcaatggaa
     3781 gtgcgtagct ctgtggtcta tgccaccatt attgtggtgc ttgtgtttct gccattgctc
     3841 acgttatcag gcgttgctgg taaacttttt gcgccactcg gctatgccta tattagcgca
     3901 atcgtggcct ctttagtggt agccctcacc ttaacccctg ccctttgcta tttgttattt
     3961 acacacggaa aattagaaag tggtgaccca ccactcattg cctggctgaa agtacgttac
     4021 gagcgacttt taaaacaaat tgaaaaacgc cacatcatga tattgagcgt gagtacgata
     4081 atcatagcgc ttggccttgg catattgcct ttgttcaaaa gccaatttat ccccagcctg
     4141 catgaaggcc actacattat gcacatgacg gcggtacccg gcacgtcagc acaggaatct
     4201 ctacgcatcg gcaaacgcgt ttctaaggtg attgaaaata ttaaaggtgt gaaatctgtc
     4261 acgcagtggg taggccgcgc gccaaatgcg gcagacactt ttggtacgca ttacagcgag
     4321 tttgaaattg agctggggaa tgcctcaggc cctgagcaac gctacattct gaatcgtatt
     4381 cgtgaggaac tcgcgggtga ggtcgaagat gaagatggcg atggcattgc agaaattggc
     4441 tttatcggtg taaattttgc catcaacacg ttcttaaccg aacgtattga agaaaccatt
     4501 tcaggttaca ccgccgccat ggtgattaac attcatggcc aagatttaga tgcacttgat
     4561 cgcgacgcac gcgaagtagc cgagctatta agcagcattt caggtgccgc agacattcaa
     4621 attcaatcac cgcctggcac cccagagctc accattcggc tcaggccaga acgcttggcc
     4681 atgtatggtt tgcaatcgct tgatgtactc gaaaacatac aggccgccta cgagggcgtg
     4741 caggcaacgc aaatttacca aggtaaccaa gtcaccagcg tgaatgtgat gtttacgcac
     4801 aatgtgcggg atgatttgcg tgaaattggc ttattaccac ttaaaaatcc tgatggaaaa
     4861 atgatactcc tgaaagaagt tgccaatatc acacaagaaa atggccgctc aaaaatcttg
     4921 catgcgggcg ccaaacgcat ccaaaccgtc acctgcaaca ttcaagaccg cagcgtgaca
     4981 agttttgccg atgaattaaa aaaacgaatc aataaagagg caaaactcac gccgggcaac
     5041 tttgtaactt acacgggtga agctgaagcc aacgcacaat cgcgtgaaga cctcattgtg
     5101 cattcgctgt tagccggtgt gggaattttc ttgatgctct acattgcctt tggccgcctg
     5161 cgcaatttgc tgctgacgtt tgccaacctg ccctttgcct taattggcgg ggtgattgcc
     5221 gccatgttta ccggtggctg gatttcgctt ggctcattgg ttgggtttgt cacgctgttc
     5281 ggcattacgc tgcgcaactc gattatgatg gtgtcccatt atcagcattt ggttgatgaa
     5341 gaacattgtg tttggggctt agaaacctgc atacgtggcg cttctgagcg cctgccctcc
     5401 attttaatga cagcgcttgt taccgcgctc ggtttgttgc cactcgccat gggtagcggc
     5461 cagccaggtc gcgaaattga agggccgatg gccaccatta ttgttggcgg cttggtcact
     5521 tctaccatat taaacttgct gattctacct accatcatgc tgcacttcgg taagtttgaa
     5581 aaacccatgc aggtttagtt tttaggtgtt ttcaaatcct gttttattca attaaactgc
     5641 gctaaaatcc cccgagaaaa aaataatgcc ctggaagcct tgtaaataaa ggcttccagg
     5701 gcatcgaaaa aggttgagtg ttaaaaacac ctgtttggat ggtttttaac gcaaaaaagt
     5761 cacacctaaa taaatgtgac gattacaccc taaacttcat cgtattttat atatttaaaa
     5821 cgcacatcat cattgggcgt acctgtgagt accccacctt caagcgctgg ctgccagccg
     5881 atgacttgct caattttgtg cgccaactca aagtctttat cggtaatgcc tttcgcgtca
     5941 tgcgtgcaga gtttaacaat cacaaacgca tatgacaccg ttaaatcggg gtgatgccag
     6001 gccgcttcag ccaaatggcc aatcgtgttt accaccatga gtgtggcttt ccagccgctg
     6061 gttttatatt tacggcgaat ccagccgttt tctaaaaacc aatgtggtaa ttctttagct
     6121 aatttctgtt gaacttcatc atccgtgtaa gctttaattt ttgtcattcc attctcctat
     6181 caaactacag atgtgctgag ttgcaggcct ttagcttgca atgcgtctac attgacgcct
     6241 tgtgcaaaga ttgcataatc tgctttatta accacccaag tttggtaact ttggccgtga
     6301 atatagttga taaattgctt atcaatgagt tcatcgtcca ccaaatgttg caagcgcgtg
     6361 ggtgcattgg tttggtaggc caacaatgct ttggatttca ccagtatcaa ttgctcggca
     6421 ccgagtttat tggcgagcca cgcactgagg ctgtcagacg tgatttccca gtttttggga
     6481 atcgtgtcat cagccagtac cattttactg ggcagccaga caatggcacg atgttgccag
     6541 ccacgctctg aaatttccaa ttcactgcgc gctgtcacca gcgctgggtt aagccctgtc
     6601 agcaacaagc catactgatc catcgctaac aaggccaact ggtgggctaa cgcatcactc
     6661 acattggcaa tctgctgcgc ttcacgtaca gcattggcaa acaaaccacc gcctggcaca
     6721 atcaccactt taccatcact aaacttcact aaagtctcaa gccagtttgc caattcaggc
     6781 gcacccagca agcttccgcc taatttaaca acccacatcc ctagtccaat ttagcatttg
     6841 ttaatccact ttaaaaaatt gcatcaaccg caacatgctt gaagctttat cgagtgtttc
     6901 aatatactct ttttccatgg tggaatctgc cacaatgccg ccgcccgcat aaaaactcac
     6961 ttcgttttgg gtatataccg ccgtgcgaat ggcaatattg gtatccatat tgccatcaaa
     7021 gccaatgtag ccaattgcgc cgcaatatag gccacgcggg ctgggttcga gttcatcaat
     7081 aatttgcatg gcacgtagtt tgggtgcacc ggtaattgag ccacccggaa aacaaccgcg
     7141 caacaaatct agcgccgatt taccttgtgc aagttttccc gtcacaatac tcaccaaatg
     7201 atgcacgttg gcaaagcttt gtagttgaaa taacttttca gcctttaccg agccaatttc
     7261 gcagtttttg ccaatatcat tccgcagtaa atcgacaatc atcaaatttt cagcgcggtc
     7321 ttttaagcta ttttgcagct caattgcttg tttggcatcc tcaatcgcat catcagaacg
     7381 cgggcgtgtg cccttaattg gccgtgtttc cacatgttgg ccgaccacct gcaaaaatcg
     7441 ctcgggggat gcagacagca cttcaagatg cggcaactgc atatatgcca taaagggtgc
     7501 gggggaaatt tcgcgcagtt ttttgtaagc taaccagcca tcgccttttg cctgtgctga
     7561 aaaacgctgg gctaaattta cctgatagca atcaccctct aaaatgtagt ttttcacttt
     7621 atcaaaagcg tgcgcatagc gtgcttttgt catattagaa attacatccg ttgtcacttt
     7681 aaatgcttca acctttttag caccgctgga agccttgtaa aataaggctt ccagggcatt
     7741 aaatttttct cgggcgtttt catggcaaaa atgtgcgatc aaatgcgccg ttttttgacg
     7801 atgatcaacc actaccgccc aatcataaat gccaaccatt aattctggaa tcgcgtccat
     7861 tgatttgggg gtgccttgta tatctacact gggtagcttt tcaaaatata gcgctaaatc
     7921 atagccaaaa taacccacgg ccccacctga aaaaggacta tcagtgctgg cattttctga
     7981 tgagatatat tgtgcaagcg cacttttaat gagctcaaat ggattttctt cagaaacttc
     8041 tgttaaacca ttttctgtaa tttcagtcct tttaccgtaa gttacaaagg ttttcacagg
     8101 agaagccacc aaaatgtcgt atcttccata ctgacttcct ggctcacctg tttgcggatt
     8161 aagggcttgg ccgctatcga gcatcatcgg ccaatccaga tgcgcgatac attcaaatag
     8221 ctttgcgcta tcgtggtgat aaggaagtgc tttttttaac atgccaaccc caaccctgca
     8281 acagcaaacg caggaaagca tacggctgca ctgcttgtta attcctcatt ttcagcagca
     8341 atataatgcg tgaccgaaac gtattgtagc cctagccgct gtgcgagttc tgccgctaaa
     8401 aattccccag ccccgcaacc aatcacctgt aaagattgct gatcttgcag gcgtgaaatc
     8461 tggcgtaaca tggcaagctg taattgattc aactgcgcag ttttaaagct gtgcgccaac
     8521 tgccgccacg cgctgagtgg cgcatcggtg gcatccatgc ccaccatccg cgcaatgcgt
     8581 cttgcgctgg cttccaccga tttttctgca tgatctgacg tgtcgctcat gttatcgtgt
     8641 agcggcaatt cgcccgtgag ggtatacaca tccgctgttg tggcaaagtg ttctgcggcg
     8701 acattcaccc aatcaccgtt aaacggtatt ttttgcgcaa gcgccatcag cggcgtgcgg
     8761 acgactcctg tataaaccag ctcatcggtt tttaatcgcg cagcatcatt aaagcccagc
     8821 acttgcggag cgtggctgtg taaaatcgca aggtcggtag tggtgctacc aatgtccaca
     8881 aacaaagcct gttttacctg ttgcgccaga tattgcaagc tcgccagcca gttcattgag
     8941 gcaatgtcat gcgcatggtg ttcaacaaga tttagcggta caaaaccagc gtttccagca
     9001 aaaaatctga cttcacccgt taattttttc gcggccaatt gcgcaatttg caccacgccc
     9061 gcctgccggt tagaaaaaat gtcagccagt tcacccgtca tggttacagc atgctgcgtg
     9121 gcctgcattt ccagcaatac gtcatcaata gccgttgcaa gttcattaag gccacgccat
     9181 agcgggcaat acacttgctt ggctgccagc accttgcctg ttgcatcgcg cagcacagct
     9241 ttaaggtgtg cgccgccaac atcccaacca attacgctat tgtttttcac cattgaatct
     9301 caacgatatt tttttgtata cgcggcattt taaaagattc atcacactga ttatcgagaa
     9361 tcaatttagc cgcgttacag cctatcgcct cagtcagcgc aacataactc gtggtaaggc
     9421 gcggattaat atcaatgaca taaattttat ttgcttgcgt gtcgatgatg acgtccaccc
     9481 caacatagcc cagcgcatca ggcagctctt tggccagttt acgcatgagt gtttcaaaac
     9541 gctgccagta agccaccatg ccgttgacgg tgattcccgt cagtttaaac agctgacctg
     9601 tttggcagat atgttgttca ttacagcaaa gtagccaggc tagcccacca cccccactta
     9661 aatacgcaaa gcttgcgttg atgcctgatt gataaggctg cgcaaaatac agcagataac
     9721 gctggtcttc ttgcagccaa tcccttagtg cggtcagcga atcaaacaag cggatacctt
     9781 cgcaccctgc cccgtcttca ggttttgcca gccatttggc cacttttaac gtggacattt
     9841 cttcaaaaaa ggctggctga atcaagtctt cacccgagta cacgggtaag gtgtgaattc
     9901 ctgccgcttg caaggtatca aacgttaaac ttttgctggt gccaatcagc gtggcatcaa
     9961 atccacagcc taaaaactgt gcgccgtgtt ctttgtcttc ttccgctagg cacagttcgg
    10021 tcaaccctag caaagcgcca tccgtttcag gcgcaataac ccagactaaa tcagcctgtt
    10081 ttagtgcctt tttaaaggtt tttttaaagt cactggcaat caccggtaaa cttttacgcg
    10141 caagtgctgg ctgtggcaag cgtgcatcat gcgtcgtgat gagagcatat tgcttcattt
    10201 gatttaagtc acgcaacaaa gcatcgcgca ttaatgtgcc ttcatgcacc agagattctg
    10261 gcagtgcttc atcacttaaa ccgcctccag tgataaactc acagacaaat aatttttctt
    10321 gtttttcttt catgagtttg aagatgcaaa taatccccgt gattgattta ttaaatggcg
    10381 ttgtggtaca cgccaagcgt ggcgaaaggc aacaatatca gccaattcaa tccaaactat
    10441 gtgattcaca cgcaccactc gccattgtca gcgctttgct cgacaacatg ccgtttacgc
    10501 aattatatat cgcggactta aatgctattc aaaaattaga ggcgcaatac acgacaaatt
    10561 acagtgccat tgcagcgatt gcaaaaaaat atccccagat aacgctgtgg gtcgatgctg
    10621 ggattagtaa caatactgaa ctgcagtatt ggcaaaagtt gaaagtgcga ctggtaattg
    10681 cgagcgaaaa ctttaaaacc attgcgcaat atcatagttt gaacttggct gaaacaccgc
    10741 atattttatc gctggatttt tttccgcaag gctatgcggg gccgctagaa ctgcttgcaa
    10801 attgtcagca ttggccgcaa gacgtgattg tgatgtcgct ggaaaatgtg ggggcaaatg
    10861 caggcgttaa tacagcatta ttgatggaaa taaaagcacg tgctcaacag cacagcctta
    10921 ttgctgcggg cggaatcaga aatattgacg atttaagagc gctcgaggca ttaggcattc
    10981 agcaggcgct catggccacc gccctgcatc aacaacaaat cactgcggca caacttgcaa
    11041 agctataagc accaaacccc gattacaaag cccaactagc aagctgggct ttgtacaagc
    11101 ataacaattc acgaataagc tcactcgcga acaccaagcg cttactttgc ttgaaacggg
    11161 tgtctggttt cctgatattt ttctataatt gtctctaaat taggttcacg atttaccgca
    11221 ttcttaatgg cttgcttcat cgccaagtag ttccattcct gcacacggtc atccatatcg
    11281 gctctggcgc taatataaac gcctacacag ataaaaatat catcgacttc gttttttggg
    11341 attgtgccat caaccacaca atccattacc gccatggcaa tgccatgttg cgccgggcca
    11401 aacatttgca cggcttgttt ctcattcttg atggtgactt tgttaaacat cacggttgct
    11461 ggcttgcagg gcaaattagg ggcaatcagc gccaacaagc cgtttacccc cactttttgg
    11521 tctgttaaag tacggcaaaa tgcttcttca gcagtgctgc cgcgcggccc cataattaaa
    11581 tcaatatgcg ccacattgcg taaatctgga caattttcta aatttggacg attctcaatc
    11641 accagcgcct caccgactaa cacacggtct acgacagcca tttttattct cctttttttg
    11701 cacgttttgt aagaatagag ttaaacgcat actgcgtata agtacgactg accaatgtag
    11761 taaaagtgca gaatataagt gcgcggttgc gcttgctaaa agagcacatc acactaaaac
    11821 agaacgtgaa atcgctacga gaaaaaaacg ctgccctgcg agcctttatt tataaggctt
    11881 acagggcagc ggaaaaggtt tggttaaata atgaaggaaa tattcaatgt ttaagccgtt
    11941 gcatataggt gttcgcccat agtgcgcagc cgctttattg aatcttttat tgcattattt
    12001 tttacctagc attactctaa attggcatga attgcacgca ttccttcttc agttaagtct
    12061 tttgcaaaaa tcaaatcagc aatagcggct tctaaaaaga taagggttga aagctcaaac
    12121 tgtgagccca ttggcatccc tttagtgagc gggaagctat tgtcattacc gatttgaatc
    12181 actaaatcag ccacatccgc cattgcagaa gatttcttca ttgaaatcac cgctaatttt
    12241 gcacctactg atttcgcttt tttcacgaat ggtaataaag tttcagtacc gccagaacct
    12301 gaaaccaaaa tcaataagtc gcccgcttta atggctggcg tcacaacttc acccaccatg
    12361 cttacgttgt aacctgcatg taccaagcgc attgcaaaaa agcgcgaaac caataaagaa
    12421 cgacctgcac cgccaataaa tgtacggcct gcaccttcaa ctaattttaa aagttctgct
    12481 gattttgcat tatcagtttc agccaaaatg ctggttaatt tatctaaaat caatttttga
    12541 ctactactca tgatatttcc ttatttaatc gaatgtgatg caaaaaattt atgataaaaa
    12601 aacccgacac atttctgagt cgggttttta attactcatc tcaataattg cttattgatg
    12661 tcaggtttaa ttaaccgtga gcaatttttg tgatttctgc agcagcagca gcaggatcag
    12721 cagcgccgta gattgcagca ccagcaacga tgatagtcgc gccagcgtca cgtacttgag
    12781 cagctgttgc agctttaacg ccaccagcca ctgaaatttc aacgcctaag ttcaaaccag
    12841 caaccaaagc taagtcagca aatggtgttt ggccagcagc ttgttggtct aaaccagtgt
    12901 gcacgccaat gatgtgtgcg ccagcagcag caacagtttt agttacagca gctttatcag
    12961 caacgttgat caagtcaact tgtgcttttt tgccgtattt gttagcaaca tcgattacac
    13021 ctttgattgt accgatatca gaaataccta atacagtcac gatgtcagca ccagctttga
    13081 agaatggctc agcttcgtag aaaccagcat cagctgtttt caagtcaact aagattttgt
    13141 tgtttgggaa tttagcacgc aatacttcaa gcaatttgat accgttgtgc ttgatgcatg
    13201 gtgtaccgat ttctaagatg tcaacgtgtg gagcaaccaa tgttgctaat gcaactgtgc
    13261 cgtcaaaatc taatgaatct agtgccattt gggttaatgc catggtgttt ctccgtttaa
    13321 tagtaatt
//
LOCUS       DAOQSI010000042        13237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_3752_length_13237_cov_5.478683, whole genome shotgun sequence.
ACCESSION   DAOQSI010000042 DAOQSI010000000
VERSION     DAOQSI010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 13237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13237
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3752_length_13237_cov_5.478683"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PKL42_04285"
     CDS             complement(<1..395)
                     /locus_tag="PKL42_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66325.1"
                     /translation="MIHHISIPAKDPLHVTKVLVELFGGVLTRFGPYRDSYIAWSGDE
                     YGTAIEVYPVGTEMFPDAGGGQANFRHNGNASPFVATHATVSVDRSKEEIMSLAQREG
                     WRAIELSRGSFRVIEFWIENHVMLEVTTE"
     gene            complement(479..961)
                     /locus_tag="PKL42_04290"
     CDS             complement(479..961)
                     /locus_tag="PKL42_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66326.1"
                     /translation="MSKHQIDQKRRSILTAGVISALAFMSGCATQNKKTGPIQVDVSG
                     VKDVPSLIAAIKKSAGSSLPASITDETFYREFANSFVKNARAAAELGYSIPKWVLDKL
                     PPQRKVVLPFLGVAVFMVAGVALAVPVSTIIIAVLASIILLGSAIISALSALFASDPV
                     "
     gene            1100..2092
                     /locus_tag="PKL42_04295"
     CDS             1100..2092
                     /locus_tag="PKL42_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HNU66327.1"
                     /translation="MLQENTPTNPQAPKLLDVVRDKIRVKHYSIRTETQYLQWVKRYI
                     LFHHKRHPREMGGKEVEAFLSHLATHGHVSSSTQNQALSALLFLYREVLGQSLPWMDD
                     VIRAKKPQRLPVVLSKQEVGKILACMQGTHGLIARLLYGTGMRIMECCRLRVLDIDFD
                     RGEILIRNGKGAKDRVTMLPNALVEMLKTHLIWRKTVFEADAAKGKAEVFLPDALDKK
                     YINAATSWEWQYVFCSGSYSVDPRSGRERRHHLDEKLVQRAVKKAVANAGVVKLATPH
                     TFRHSFATHLLENGYDIRTVQELLGHSDVSTTMIYTHVLNKGGRGVSSPLDGLA"
     gene            complement(2143..2514)
                     /locus_tag="PKL42_04300"
     CDS             complement(2143..2514)
                     /locus_tag="PKL42_04300"
                     /inference="COORDINATES: protein motif:HMM:NF025742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HNU66328.1"
                     /translation="MNNLHQASQINGFDYVVTDEQMRLYAKMSDLERLEWVESARIFT
                     LLSQTPVTKVRHEALRKGLTQNNTLKKIKRGRPAGTTKKDTKVAVKLRLNPEVLKAFR
                     ATGRGWQTRINDLLVSYINSL"
     gene            complement(2507..2992)
                     /locus_tag="PKL42_04305"
     CDS             complement(2507..2992)
                     /locus_tag="PKL42_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66329.1"
                     /translation="MFYIELFKVLEAANIDYLLVGGLAMNLHGVPRMTMDIDLIIALN
                     TENVAKFEACMKNLDIFPNVPVKLSELVDTKKREALINDKHLIALSLISKKPALPAVD
                     IVIFHPLNFNEAFKNRIVRDIAGTRVFLASIEDMITMKKSAGRMQDLADVKHLERFLN
                     E"
     gene            complement(3157..3546)
                     /locus_tag="PKL42_04310"
     CDS             complement(3157..3546)
                     /locus_tag="PKL42_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HNU66330.1"
                     /translation="MFNQNTLCACESGKPYANCCEPYHLGLATPNAEALMRSRYTAFV
                     LKFEAYLLATWHPTTRPTSLNLAEDPPTKWLGLQIKRAENVNENFATVEFVARYKIAG
                     KATRLHETSQFERIDGRWYYLTGEYHN"
     gene            complement(3539..5110)
                     /locus_tag="PKL42_04315"
     CDS             complement(3539..5110)
                     /locus_tag="PKL42_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNU66331.1"
                     /translation="MSLQEEITRRRTFAIISHPDAGKTTLTEKLLWFGGAIQVAGEVR
                     GRKASRHATSDWMELEKQRGISVTSSVMQFPYREHMVNLLDTPGHDDFSEDTYRTLTA
                     VDSAVMVIDSVNGVEAQTIKLLNVCRMRDTPIITFINKLDRESRAPIELLDEIETTLG
                     MECAPMTWPIGMGKSFRGVYNLYTDTISFFDPRAEKGTSEVIKGLDNPRLDELLGHVA
                     GDLRIDIELVRGASHAFDAARYLSGKQTPVYFGSAINNFGVQSLLDAVVDLSPAPLAR
                     NTTSRLVAPDEAKFSGFVFKIQANMDPKHRDRIAFLRLCSGRFERGMKIKQVATGKQM
                     AVNNAITFMAQDRTTMDEAYSGDIIGIPNHGTIKVGDTFTEGENLKFTGIPSFAPEFF
                     RRARIKNPMKMKQLQIGLKQLSEEGAAQLFRPLLSNDLILGAVGMLQFEVVAHRLEHE
                     YSVDMAFEPYDCYTARWLRGKDADLKAIADKYGFNVALDGADNYVYLAPNRVNLQMAQ
                     ERYPDIEFAETRELV"
     gene            complement(5448..7424)
                     /gene="acs"
                     /locus_tag="PKL42_04320"
     CDS             complement(5448..7424)
                     /gene="acs"
                     /locus_tag="PKL42_04320"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNU66332.1"
                     /translation="MSIESVLHENRVFEPSADFVKQANVSGMANYKAICAEATADYEG
                     FWAKLARELLIWHKPFTKTLNEDNAPFYKWFEDGELNVSANCLDKHLNTIPNKVAIIF
                     EGDDGTSKNVTYKALYHQVCAFANGLKKLNLNIGDRVIIYLPMGIEAVVAMQACARIG
                     LIHSVVFGGFSAKSLNERIIDAGAKAVITADGQFRGGRTLPLKAAVDEGIAMGGCDSI
                     EKIVVLKKTGQEVAWNSKDMWWSDLIAGCADTCEPVMVNAEHPLFLLYTSGSTGKPKG
                     VQHSSAGYLLHAINTMRWTFDIKDNDVFWCTADVGWITGHSYVAYGPLAIGATQVIFE
                     GIPTYPNAGRFWQMIEKHNVTIFYTAPTAIRSLIKASETTPEVHPKNYNLNSLRILGS
                     VGEPINPEAWMWYFENVGGSRCPIVDTFWQTETGGHVITPLPGATPLVPGSCTLPFPG
                     IDIDVVDETGKDVPWGTGGLLVIKKPWPSMIRTIYNDPERYKTSYYPIDLGGKTYLAG
                     DGAVRDAVTGNFTIMGRIDDVLNVSGHRLGTMEIESALVSNHLVAEAAVVGKPHDVKG
                     EAVVAYVVLKGARPTGDEAKKIVAELRDWVGKEIGPIAKPDEIRFGDNLPKTRSGKIM
                     RRLLRTLAKGEEITADISTLDNPAILEQLKEVVK"
     gene            7513..10263
                     /gene="glnE"
                     /locus_tag="PKL42_04325"
     CDS             7513..10263
                     /gene="glnE"
                     /locus_tag="PKL42_04325"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HNU66333.1"
                     /translation="MQTLSDHLIHPEFSALQRAYLRRLEVCSPFVSRMLAKDANLLED
                     LLKNLHIPYTLEEMQSFIAKQAITDENSLKKSLRQLRKHVMARMICRDLNGFADLHEV
                     MHTTSTLAECVINTAIDFLNTWLVESYGQPVDGQGYPQHLIVIGMGKLGGGELNVSSD
                     IDLIFAYAAEGETTGEKSISNQDFFVRLAKKLIAVIDEITEDGFVFRVDMRLRPFGSE
                     GALVSNLDALEEYYQNNGREWERYAWIKGREVTGGNEVSHLLKPFVFRKYLDYGVYES
                     MRNLKRQIQRDVNSKGMHDNIKLGRGGIREIEFIAQVYQLIRGGKDASLQIKPTLSVL
                     NLLKNKGLMSEQAVKDLTEAYVFLRNLEHRLMYVEDAQTHELPTTDEAKARIAEAMNF
                     STIDQHGWPAFLATLEAHRALVQQHFDATFNEEDVGQDTLATENSIWGGLLAENDALN
                     ALHQLGFGDATEVFRRITMLRQGSRYLQLPDMSRQRFDALMPYVIAQSGKMQNADVTI
                     TRVIDLLESICRRASYLALLAEHPQSMQLLVKLCGSSPWLTNYLTQHPILLDELLDTN
                     TLYAKPDFAALHKELLERLTEAEHDIERQMDVMRHFKHACVFRFAAQDINGELTIETL
                     SDYLSDLADLIMRVALKVIWPYVRGKHCDEPKFAVIGYGKLGGKELGYASDLDIIFLF
                     DDNATEAGEAYVRFAQRISNWFNSLTSAGLLYETDLQLRPDGNSGLLVSTVEAFREYQ
                     RNKAWVWEHQALSRARFVAGDTNIGAAFEKIRVEVMTQARDAEKLKNEVLRMREKMRD
                     AQHFIAGQFDIKHSVGGIIDVEFLVQYLVLRYASNYPQLTANIGNIGLLKRMAELGII
                     DVALAENVAVAYREFRHIQHRLKLQDAARLSVELNQVITHADAVRFLWREVFGAENTQ
                     NS"
     gene            complement(10457..11494)
                     /locus_tag="PKL42_04330"
     CDS             complement(10457..11494)
                     /locus_tag="PKL42_04330"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNU66334.1"
                     /translation="MSNTLTTAETNTLLSKLVALTHGGDEVSLEMKLLGLLSDFVGPV
                     NNHENAVAIYHLIHLEKQRFSRHTQQQHETNIKLTKAHQQAVMHCFTEGVVGLYQPAN
                     APHVKLYPIKDFAEKVVSVVEITMPSQEAGSVDAKADTAIGMMLEIYQNFTKLMNDNE
                     CDTLTGLLNRKTFDSKINKIITQMRTVAKRKEDKSAQFYYLAIFDIDHFKRVNDNFGH
                     LIGDEVLLMFSQLMKQSFRDSDPLFRFGGEEFVGVFGCNHVNDINTILERFRNKMAQF
                     VFPQVGKVTVSAGYTEIQDFDNSSQLIDRADSALYFAKNHGRNQVACYEHLVATGELQ
                     VNKKEGDIELF"
     gene            complement(11619..12620)
                     /gene="dhaK"
                     /locus_tag="PKL42_04335"
     CDS             complement(11619..12620)
                     /gene="dhaK"
                     /locus_tag="PKL42_04335"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HNU66335.1"
                     /translation="MKKFINKVDDILVESLSGFATAHADLVALNLNPNFLTRKNKAHN
                     KVAIISGGGSGHEPLHAGYIGVGMLDAACPGHVFTSPTPDQMLEAATAVHADKGILLI
                     VKNYAGDVMNFEMAAEMLPFENATVLTSDDCAVINSTYTTGRRGVAGTVIVEKCVGSL
                     AETGADLAACKALGDKVNTRTASIGVALTSCTVPAAGRPTFDLNETDLEMGVGIHGEP
                     GRRREALREADAIVADCMEAILADFKTKNIATSQTEALLLVNGFGATPLMELYLLYNT
                     AAQLFTQHGIQIVRSLVGNYTTALDMAGASITLCLLDDEIKQHWDSPVHTPALRWKM"
     gene            complement(12650..>13237)
                     /gene="dhaL"
                     /locus_tag="PKL42_04340"
     CDS             complement(12650..>13237)
                     /gene="dhaL"
                     /locus_tag="PKL42_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HNU66336.1"
                     /translation="AIQRTILANEHTLESLDREIGDGDHYINMKRGASTVVDMQAELV
                     TLSPEAALNKIGLKLLSTIGGASGPLLASFFMSMSKTLKEHGDDSLPKIAAAFAAGVQ
                     AIIQRGKANLGEKTMLDVLIPVATQFESLAALGHDAKLIASALTKTAETGMLSTQDLI
                     ATKGRAAGLGERAIGHIDPGAKSCQLMIEAVCQLV"
BASE COUNT         3504 a         2937 c         3091 g         3705 t
ORIGIN      
        1 tcctctgtcg tgacttcgag catgacatga ttctcgatcc agaattcaat gacccgaaac
       61 gatcccctag acaactcgat agctcgccat ccttctcgtt gtgcaagaga cattatctct
      121 tccttacttc gatcaacaga aacagtggcg tgtgttgcga caaagggtga ggcgttccca
      181 ttgtgcctaa agttggcttg accacccccg gcgtcaggaa acatttccgt gccaactggg
      241 taaacctcaa tagcggtacc gtattcgtct ccagaccatg caatgtacga atcccggtag
      301 gggccgaatc tcgtcagcac accaccaaat agctcaacca gcacttttgt gacgtgcaag
      361 gggtctttcg cgggtatgga aatgtgatga atcatcgctt aatgctcctt gtagaggccc
      421 aacgtttgac ataaggggcg cgctttagcg cgtcccgctt gatggatggg ttgggcattt
      481 acactgggtc agatgcaaac aatgcactaa gtgctgaaat gatagccgaa cctaaaagga
      541 ttatagatgc caagacagca ataataattg tagatactgg caccgcaagt gctacaccag
      601 caaccataaa aacagcaacg cctaaaaatg gaaggacaac ctttctttgt ggcgggagtt
      661 tatcgagcac ccacttaggg attgaataac caagctcggc tgcggcacgg gcatttttta
      721 caaatgaatt tgcaaactct ctgtaaaaag tttcatctgt aatagatgct ggcaatgaac
      781 taccagccga ttttttaatt gctgcaatta agctcggaac atctttaacc ccagaaacat
      841 caacttgaat tggacctgtt tttttattct gtgtagcgca tcccgacata aatgccaacg
      901 ctgaaattac accagcagtt aggatggaac gacgtttttg atcaatttga tgcttactca
      961 taaagttcct ttgtgtttat gcctaacgta aagtaggcac caaaatcggt gcatagttaa
     1021 taaaatatag gtgtataatc tttttgtaat tcatattatc tatttgtatt gtatatttat
     1081 ttttaataat taaaccaaaa tgcttcaaga aaatacacct actaatccgc aagccccaaa
     1141 actgctagat gttgtgcgcg ataaaatacg tgtaaagcat tacagcattc gcacagaaac
     1201 gcaatacctg cagtgggtta agcgttatat tttgtttcac cataagcgcc acccgcgtga
     1261 aatgggcggt aaagaggtgg aggcgttttt gtcgcatttg gccacacatg gccatgtgtc
     1321 atccagcacg caaaatcagg cgctttcggc tttgttgttt ttgtatcgtg aggtgttggg
     1381 gcaaagcctg ccgtggatgg acgatgtgat tcgcgctaaa aagccgcagc gtttgccggt
     1441 ggtgctttca aaacaagagg tgggcaaaat tttagcctgt atgcaaggca cgcacggctt
     1501 aattgcgcgg ctgctttatg gcacgggcat gcgcattatg gaatgctgtc gtttgcgtgt
     1561 gctggatatt gattttgacc gaggcgaaat tttaatccgc aatggcaaag gcgcaaaaga
     1621 tagggtgacc atgttgccca atgccttggt tgaaatgtta aaaacacatt taatttggcg
     1681 caaaaccgtg tttgaggcgg atgctgctaa aggcaaggcc gaagtttttt tgccagatgc
     1741 gttagataaa aaatacatta acgccgctac cagttgggag tggcaatatg ttttttgctc
     1801 gggcagttat tcagttgacc cgcgcagtgg tcgcgagcgc aggcaccatt tagatgaaaa
     1861 attagtgcaa cgtgcagtta aaaaagccgt tgctaatgcg ggcgtggtta agttggctac
     1921 accgcacact tttcgccaca gttttgccac gcacttgctt gaaaatggct acgatattcg
     1981 caccgtgcaa gagttattgg ggcattcaga tgtgagtacc accatgattt acacgcatgt
     2041 gttgaataaa ggcgggcgcg gcgtgagtag tccgttagat ggtttggctt agtgtttata
     2101 aagaattttt ggtattttta tcacaccaaa ctcaaacgtt cgctaaagcg agttgatata
     2161 acttaccagt aaatcgttta ttcttgtttg ccagccgcgc cccgtggcac gaaaggcttt
     2221 caaaacttca gggtttagcc tgagtttaac ggccactttt gtatcttttt ttgttgtgcc
     2281 tgctggtcgc ccgcgcttaa tttttttaag tgtattgttt tgagttaagc cttttcttaa
     2341 cgcttcatgc cgcacttttg ttacgggtgt ttgcgataaa agtgtaaaaa ttctggcact
     2401 ttccacccat tctaaacgtt ctaaatcact cattttagcg tataggcgca tttgctcatc
     2461 ggtcaccaca taatcaaaac cattgatttg acttgcttgg tgcaaattat tcattcaaaa
     2521 atctttctaa atgcttaacg tcggctaaat cttgcatgcg cccagcagat tttttcatgg
     2581 taatcatatc ttcaatactc gccagaaaaa cgcgcgtgcc tgcgatgtct cgtactattc
     2641 tgtttttgaa agcctcatta aaatttagtg ggtgaaaaat cacaatatca acggcaggca
     2701 aggcgggttt tttgcttatc aatgaaagcg caatcaaatg tttgtcgttg atcaaagcct
     2761 cacgtttttt ggtgtctact aattcagata atttaacagg cacgtttgga aagatatcaa
     2821 gatttttcat gcatgcttca aatttggcta cattttcagt attcaaggcg ataattaaat
     2881 caatatccat cgtcatgcgc ggaacgccat gcaagttcat ggctagccca cccacgagca
     2941 aatagtcaat attggctgct tctagaactt taaataattc aatataaaac attatttaaa
     3001 tgtacaccca tttaattgta gttacaagct tttaatagca tggatattgc aaagagtgtt
     3061 ctgcatagcc ttgaatattt gaagggattc ggtgttttag ctcagttcac tgattttaaa
     3121 tttatacaaa aaatcagtac gatttcgctt caaatattag ttgtgatact cgcccgttaa
     3181 ataataccag cgcccgtcta tgcgctcaaa ctggcttgtt tcatgcagtc tggtggcttt
     3241 acctgcaatt ttatagcgtg ctacaaattc aaccgttgcg aagttttcat ttacattttc
     3301 tgcgcgcttg atttgtaacc ccagccattt tgtgggtggg tcttcagcta aattaagcga
     3361 ggtagggcgt gtagttgggt gccaagtggc tagcaaatac gcttcaaatt tcaacacaaa
     3421 agccgtgtag cgtgagcgca ttaaggcttc tgcgttgggc gtagcaaggc ctaagtgata
     3481 aggttcgcag cagttggcat aaggtttgcc actttcacat gcgcaaagcg tgttttggtt
     3541 aaacaagctc gcgcgtctcg gcaaactcaa tatctgggta gcgctcttgc gccatttgca
     3601 agttcacgcg gttgggtgca aggtaaacat aattatctgc gccatcgagc gccacgttaa
     3661 agccatattt atcggcaatc gcctttaggt cagcatcttt gccacgcagc cagcgcgcgg
     3721 tgtagcaatc gtaaggttca aatgccatgt ctacgctgta ttcgtgctct aggcgatgcg
     3781 caaccacctc aaattgcagc atgccaactg cgcccaaaat caaatcattg ctcaataatg
     3841 ggcggaataa ctgcgccgcc ccttcttcag aaagttgttt aagtccaatt tgcaactgct
     3901 tcattttcat tgggttttta attctagcgc ggcggaaaaa ctctggcgca aaagatggga
     3961 ttcctgtgaa cttaagattt tcgccttccg taaacgtatc gcccacttta atggtgccgt
     4021 ggttaggaat accaataata tcgcccgaat acgcctcatc catcgtggtg cggtcttgcg
     4081 ccataaaggt aatggcgttg ttcaccgcca tttgtttacc cgtagccact tgtttaattt
     4141 tcatgccgcg ttcaaaacgg ccagaacaca ggcgcaaaaa cgcaatgcgg tcgcggtgtt
     4201 tggggtccat attcgcttga attttaaata caaagcctga gaattttgct tcatccggcg
     4261 ctactaagcg cgaggttgtg ttacgtgcca atggcgcggg ggataggtca acaacggcat
     4321 ctagcaacga ttgcacgcca aagttgttaa tggccgagcc aaaatacacg ggcgtttgct
     4381 tgccacttaa ataacgcgcc gcatcaaagg catgcgatgc accgcgcacc agctcaatgt
     4441 caattcgcaa atcgcctgcc acatgcccca gcaactcgtc taaacgtggg ttatctaaac
     4501 ctttaattac ttcagaagtg cctttttcag cccgcggatc aaaaaacgaa atggtatcgg
     4561 tgtataagtt atatacaccg cgaaatgatt tgcccatgcc aatcggccac gtcatgggcg
     4621 cacattccat gcccagcgtg gtttcaattt catctaacag ttcaatcggc gcgcggcttt
     4681 cacggtcgag cttgttaata aacgtaataa tcggcgtgtc gcgcatgcgg cacacgttca
     4741 gcaatttaat ggtttgcgct tcaacgccat tcaccgagtc aatcaccatc acggcagagt
     4801 caacggccgt caacgttcta taagtatctt ctgaaaagtc atcatggcct ggcgtgtcga
     4861 gcaaattcac catgtgttca cggtaaggaa actgcatgac cgatgaagtc accgaaatgc
     4921 cgcgttgttt ttcaagttcc atccagtcag aagtcgcatg gcgtgaagcc ttacgcccac
     4981 gtacttcacc cgctacttga atcgcgccac caaaccataa tagtttttcg gtaagcgtgg
     5041 ttttacccgc atcggggtgc gaaataatgg caaaggtgcg gcgacgggtg atttcttctt
     5101 gaagagacat aagttaagca aacaataaac acaaagccag cattttaccc gaaatatcca
     5161 aatgccttaa aactacacat tattttagaa attatcggta gttaaataaa gtttaaataa
     5221 atggaatata cgcccgagag aaaaaacatg ctctgcaagc ctttattgat aaggcttgca
     5281 gagcatcgaa aaaggttgca ttaaaaaatg gtgtttttga atggtttttc agatgtaatg
     5341 ttgagattaa aattgagaac gcagtgcgtt gagcgaaacc taataatccg ccgaaagggt
     5401 aatgttgcat gcggcggaat aacaaagtgt attccgccca tttaaagtta tttcaccact
     5461 tctttcaact gctctaaaat tgccgggtta tccaaagtcg aaatatccgc cgtaatttct
     5521 tcgcccttag ccaatgtgcg tagcaagcgg cgcataatct tacctgagcg ggttttgggc
     5581 aggttatcac caaagcgaat ttcatcgggt ttggcaattg ggccaatttc tttgcctacc
     5641 caatcacgta gttcagcgac gatttttttc gcttcatcgc cagttgggcg cgcgcctttt
     5701 agcaccacat aagctaccac ggcttcacct ttaacatcat gcggtttgcc aactacggcg
     5761 gcttcagcca ccaagtggtt tgaaacgagc gcggattcaa tttccatggt gcctaaacgg
     5821 tggccagaaa cattcagcac gtcatcaata cgccccatga tggtgaagtt gccagtcact
     5881 gcatcgcgca ctgcgccatc gcccgctaaa taggttttgc ctcctaagtc aattgggtaa
     5941 taactcgttt tgtagcgttc agggtcgtta taaatcgtgc gtatcatcga aggccatggt
     6001 tttttaatca ccagtaagcc gcccgtgccc caaggcacat ctttgccggt ttcatcgact
     6061 acatcaatgt caataccggg gaagggcagc gtacaggagc cgggcacgag cggtgtggcg
     6121 cctggcagtg gcgtaatcac atggccgcca gtttctgttt gccagaaagt atccacaatt
     6181 gggcagcggc taccgccgac attctcaaag taccacatcc aagcttctgg gttaatgggt
     6241 tcacccaccg aacctaaaat ccgcaggcta ttgaggttgt aatttttggg gtgcacttca
     6301 ggggtggttt ccgaggcttt aatcagcgag cgaatggccg ttggtgcggt gtagaaaatc
     6361 gtcacgttgt gtttttctat catttgccaa aagcgtccgg catttgggta ggttggaatt
     6421 ccctcaaaaa tgacttgtgt ggcgccaatg gcaagcggcc cataggccac ataactgtgg
     6481 ccggtaatcc agcctacatc ggccgtgcac caaaacacat cattgtcttt aatgtcgaaa
     6541 gtccagcgca tggtgttaat cgcatgcagc aaatagccag cagagctatg ttgcacgcct
     6601 tttggcttac cggttgagcc tgaggtatag agtaaaaaga gcggatgttc agcattgacc
     6661 atcaccggct cgcatgtgtc agcacagcca gcaattaaat cactccacca catatctttt
     6721 gagttccaag ccacttcttg gccggttttt ttcaatacca caattttttc aatactgtca
     6781 cagccaccca tggcaatgcc ttcatcgact gcggctttaa gtggtaaggt tctaccgcca
     6841 cgaaactggc catcagcggt aatgacggct ttggcacctg catcaataat gcgctcattt
     6901 aagcttttag cagaaaaacc accaaatacc acagagtgaa tcaggccgat gcgcgcgcag
     6961 gcttgcatcg caaccacggc ctcaatgccc ataggtaaat aaataatgac gcggtcgcct
     7021 atattgagat taagtttttt caacccgttg gcgaacgcac atacttggtg gtacaacgct
     7081 ttgtaagtaa cgtttttgct tgtgccatcg tcgccttcaa aaataatcgc gactttattg
     7141 ggaatggtat ttaagtgttt atctaaacag ttggcagaaa cattcagttc gccatcttca
     7201 aaccacttgt agaagggcgc gttgtcttca ttgagtgttt tcgtaaaagg cttgtgccaa
     7261 atcaataact cgcgtgctaa tttagcccag aatccttcat aatcagcggt ggcttcagcg
     7321 catattgcct tatagtttgc cattcctgat acattggctt gttttacaaa atctgcactt
     7381 ggttcaaaaa cgcggttttc atgaagaact gattcgattg acatgctgcc cccaaaaagt
     7441 ttttaagctt aaaatttagt tagaatggta ttttaaacgc catattacat taaaaaaccg
     7501 aagcaaacac ccatgcaaac attaagcgac cacctgatac accctgaatt ttctgccttg
     7561 caacgtgctt atttacgaag gttagaagtt tgcagtccgt ttgtgagccg aatgttggca
     7621 aaagatgcca atttgctcga agatttgcta aaaaatctgc acattcccta taccttagaa
     7681 gaaatgcaaa gtttcatcgc aaaacaagca attaccgatg aaaattcgtt aaaaaaatca
     7741 ctcaggcagc ttagaaaaca tgtcatggcg cgcatgattt gtcgtgattt aaatggtttt
     7801 gcagatttac atgaggtgat gcacaccacc tcaacccttg ctgagtgcgt gattaataca
     7861 gcgattgatt ttttgaatac atggttggtt gagtcttatg gtcagccggt ggatggccaa
     7921 ggctatccac aacatttaat tgtcattggc atgggcaaac tgggtggagg tgagcttaat
     7981 gtttcatcag atattgattt gatttttgct tatgccgcgg aaggcgaaac gactggcgaa
     8041 aaatcaatca gcaatcaaga tttttttgtg cgcttggcta aaaagttaat tgcggtgata
     8101 gacgaaatca ccgaagacgg ctttgtgttt agagtggaca tgcggctacg cccatttggc
     8161 agtgaaggcg cattagtgag caatttagat gcgctggaag aatattacca aaacaatggc
     8221 cgcgagtggg agcgctacgc ctggattaaa ggacgagagg tgacgggtgg aaatgaggtg
     8281 tcgcacttgc tgaagccttt cgtgtttcgt aaatatcttg attatggcgt gtatgaaagc
     8341 atgcgcaatt taaagcgcca aatccagcgc gatgtgaata gcaaaggtat gcatgacaac
     8401 atcaagctcg gccgcggcgg gattcgtgaa attgaattta ttgcgcaggt ttatcagttg
     8461 atacgtggcg gtaaagatgc cagcttgcag attaaaccta ccttgtccgt attaaattta
     8521 ttgaaaaaca aagggttaat gtcggagcag gcagtgaaag acctgacgga ggcttatgtg
     8581 tttttgcgca atttagaaca tcgcttaatg tatgtagaag acgcgcaaac gcatgagttg
     8641 cccaccacgg atgaagcgaa agcgcgcatt gccgaggcga tgaatttttc gaccattgat
     8701 caacatggct ggccagcttt tttagcgaca ttagaggcac accgtgcgct ggttcagcag
     8761 cattttgatg caacctttaa tgaagaagac gttggccaag atacgctggc gactgaaaac
     8821 tccatttggg gtggtttgct cgctgaaaat gacgcattga atgctttgca tcaactaggg
     8881 tttggtgatg caacagaagt gttccgccga attaccatgt tgcgacaggg cagccgttat
     8941 ctccagttgc ccgacatgag ccgccagcgc ttcgatgcgc tgatgccgta tgtgattgcg
     9001 cagtcaggca aaatgcaaaa cgccgatgtc accatcacgc gcgtgatcga tttacttgaa
     9061 agcatttgcc gccgcgccag ttatttagcc ttattggcag agcatccgca atctatgcaa
     9121 ctgctggtga agttatgtgg tagcagcccg tggttgacta actacctcac gcaacatcct
     9181 attttattag acgaattgtt agacacgaat acgctctatg caaaaccaga ttttgcagcc
     9241 ttacacaaag aattattaga gcgcctaaca gaagccgagc acgatattga acggcaaatg
     9301 gatgtgatgc ggcattttaa acacgcgtgt gtttttcgct ttgctgcgca ggacatcaat
     9361 ggggaactga ccatagaaac gctctcagac tatttaagtg atttggctga tttaattatg
     9421 cgcgtcgctt taaaagtcat ttggccatat gtgcgcggta aacattgcga tgaacctaaa
     9481 tttgccgtga ttggctatgg caaactgggt ggcaaagagc ttggctatgc ctctgattta
     9541 gatattattt ttctgtttga tgacaatgca acggaagcgg gtgaagccta tgtacgcttt
     9601 gcgcaacgca ttagcaactg gtttaacagc ctgacttccg caggcttact ttatgaaacc
     9661 gatttgcaac tgcgccccga tggcaatagt ggcttattgg tgagtacggt agaagctttt
     9721 cgtgaatatc aacgcaataa agcatgggtt tgggaacatc aagcactcag ccgcgctagg
     9781 tttgttgcag gggacaccaa cattggtgcc gcctttgaaa aaattcgcgt tgaagtcatg
     9841 acgcaagccc gagatgcaga aaagctgaaa aatgaagtgt tacgcatgcg tgaaaaaatg
     9901 cgcgatgccc agcattttat cgcagggcag tttgatatca agcacagtgt gggcggcatt
     9961 attgatgtgg aatttttggt gcaatattta gtgttgcgat acgcctcaaa ttatccgcaa
    10021 ctcacagcaa atattggcaa tattggccta ttaaaacgga tggctgagtt aggcattatt
    10081 gatgtagcac tggcagaaaa tgtcgcggtt gcctaccgag aatttcggca tattcagcat
    10141 cggctaaaat tacaagatgc tgcgcgattg agcgttgaac tcaaccaagt aatcacacat
    10201 gccgatgcgg taagattttt gtggcgtgaa gtttttggtg ccgaaaacac gcaaaatagt
    10261 tgagcgatta aatagcttga attttagctt tttttgctta aatcaacctc caaccttttt
    10321 ggatgccctg cgagccttat aaataaaggc tcgcagggca gaaaaaattc ctcgagtgtt
    10381 tttgcgtgtg ttttaattaa aaaaaggcac taaaaaacgc agaaattggg tcggttttta
    10441 actgaaataa tcagcgttaa aatagctcaa tatcgccttc ttttttgttc acttgcagtt
    10501 cacctgtggc cacgagatgt tcgtaacaag ccacctgatt acggccatga ttttttgcaa
    10561 aatagagggc agagtctgca cggtctatca actggctgga attgtcaaaa tcttgaattt
    10621 cggtgtaacc cgcgctcact gtcactttgc ccacttgcgg aaaaacaaac tgcgccattt
    10681 tgtttctaaa gcgctccaaa atggtgttga tatcattaac gtggttacag ccaaaaacgc
    10741 ccacaaactc ttcaccgcca aatcgaaata gcgggtcaga atcgcgaaaa gattgcttca
    10801 taagttgcga gaacatgagt agtacttcgt cgccaattaa atggccaaag ttatcattta
    10861 cccgtttaaa gtggtcaatg tcaaaaattg ccaaataata aaattgcgct gatttatctt
    10921 ctttgcgctt ggccacggta cgcatttgcg tgatgatttt attgattttg ctatcgaatg
    10981 ttttgcgatt aagtaagccg gtgagagtgt cgcactcatt gtcattcatg agtttggtaa
    11041 agttttgata aatttccagc atcatgccga tggcggtgtc ggcttttgca tctacactgc
    11101 cagcttcttg acttggcatg gtaatttcaa ccaccgaaac gactttttct gcaaaatctt
    11161 ttattgggta tagttttacg tgcggcgcgt tagccggttg gtagaggccg accacacctt
    11221 cagtaaagca gtgcataaca gcctgctggt gcgcttttgt aagcttaatg ttggtttcgt
    11281 gctgctgttg cgtgtgtctg gaaaaccgtt gtttttcaag gtgaatcagg tggtaaatgg
    11341 caacagcgtt ttcatggtta tttaccggcc caacaaagtc ggacagcaag cctaatagct
    11401 tcatttcaag cgaaacctca tcaccgccat gggtgagcgc aacaagtttg cttaataacg
    11461 tattggtttc tgcggttgtc aaagtgttgc tcatttaagt actgttcatt taagtgctag
    11521 gttaacttag tttacttttt gatgtgaaat gattcatagg caatgagtaa gagctgcatt
    11581 gtagcttaaa aattgaccac atctaatagc ctgattggct acattttcca cctgagtgct
    11641 ggcgtgtgta ctgggctgtc ccaatgttgt ttaatttcat catccaacaa gcacaaggtg
    11701 atggatgcgc ctgccatgtc gagcgcggtg gtgtagttgc ccactaaaga acgtacgatt
    11761 tgaatgccgt gctgtgtgaa taattgtgcg gcggtgttgt agagcagata gagctccatt
    11821 aacggtgttg cgccaaagcc gttcaccaat agcaacgcct cagtttgtga ggtggcaatg
    11881 tttttagtct tgaagtccgc tagaatcgcc tccatacagt cggcgacgat ggcatcggct
    11941 tcgcgcaaag cttcacgtct gcgccccggt tcaccatgaa tccccacgcc catttccaga
    12001 tcagtttcat ttaagtcaaa ggtcgggcgg cccgccgctg gcacggtgca acttgttaaa
    12061 gccacgccaa tacttgccgt gcgtgtgttg actttatcgc ctaaggcttt gcaggcagct
    12121 aagtccgcgc cagtttcggc aagactgccc acacattttt ctacaatcac ggtgccggcc
    12181 acaccgcggc gcccggtggt atacgtgctg ttaatgactg cgcaatcgtc actggtgagt
    12241 acggtggcgt tttcaaaagg cagcatttcc gccgccattt caaaattcat cacgtcgccc
    12301 gcgtagtttt taacaatcaa taaaatacct ttgtcagcat gcacggcggt tgccgcttct
    12361 agcatttggt cgggcgtggg cgaggtgaat acgtggccgg ggcaggcggc gtcgagcatg
    12421 ccaacaccaa tatagcctgc gtgcaacggc tcgtggcctg agccgccgcc agaaataatg
    12481 gcaactttat tgtgagcctt atttttacgt gttaaaaaat tggggtttaa gttgagtgcg
    12541 actaagtcgg catgcgctgt ggcaaaacca gaaaggcttt caactaaaat gtcatcgact
    12601 ttattaataa attttttcat cgtgattgta ctttcttgat tgtgtgttct taaacaagtt
    12661 ggcaaacggc ttctatcatc agttggcagc tttttgcgcc tgggtcaatg tgaccaatcg
    12721 cgcgttcacc taagcctgcc gcgcggcctt tggtcgcaat caggtcttga gtgctgagca
    12781 tgccggtttc agctgttttg gtgagtgcac ttgcaatcag ttttgcatca tggcctaggg
    12841 ctgcgagtga ttcaaattgt gttgcaacag gaatgagtac atcaagcatg gttttttcac
    12901 ccagatttgc cttaccgcgc tgaataatcg cctgcacgcc tgctgcaaaa gctgcagcaa
    12961 tttttggcag cgagtcgtcg ccatgttctt tcagggtttt actcatgctc ataaaaaagc
    13021 tcgcgagcag tggcccagaa gcaccgccta tcgtactcaa aagttttaag ccaattttat
    13081 tgagtgctgc ttctggacta agggtgacta attcagcttg catgtccaca acagtcgatg
    13141 caccgcgttt catgttgatg tagtggtcgc catcaccgat ttcgcggtcg agggattcaa
    13201 gcgtatgctc attggctaaa atggtgcgct gaatggc
//
LOCUS       DAOQSI010000043        12933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_3924_length_12933_cov_5.313325, whole genome shotgun sequence.
ACCESSION   DAOQSI010000043 DAOQSI010000000
VERSION     DAOQSI010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 12933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12933
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3924_length_12933_cov_5.313325"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /gene="cgtA"
                     /locus_tag="PKL42_04345"
     CDS             <1..686
                     /gene="cgtA"
                     /locus_tag="PKL42_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HNU66337.1"
                     /translation="GGLGNVHFKSSLNRAPRQCTKGELGEEFELYLELKVLADVGLLG
                     MPNAGKSTFIRSVSAAKPKVADYPFTTLHPNLGVVRVDAERSFVIADIPGIIEGAAEG
                     AGLGHQFLRHLQRTSLLLHLVDIAPFDEAVDPVHEAKAIVEELRKYDEALYEKPRWLV
                     LNKIDMLQDSEQAVKQFVKDYGWKGRVFAISAISGIGCKELTYAIMQHIDDSKAQDAQ
                     ELENEISGT"
     gene            667..1785
                     /gene="proB"
                     /locus_tag="PKL42_04350"
     CDS             667..1785
                     /gene="proB"
                     /locus_tag="PKL42_04350"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNU66338.1"
                     /translation="MKSLVHDAKVIVVKVGSSLVTNNGNGLDHAAIVAWAAQIASIVK
                     QGKQVVLVSSGAVAEGMQRLGWKKRPTAVNELQAAAAVGQMGLVQMYERCFAEHDLHT
                     AQVLLTHDDLADRKRYLNARSTLRTLLDLKVIPIINENDTVVTDEIRFGDNDTLGALV
                     ANLIEADALVILTDQAGLYTADPRSNPKAEFVQHATAGDVALEQMAGGAGSSVGTGGM
                     LTKILAAKRAARSGAHTVIASGREKEVLLRLANGEVIGTHLEATQIKTVARKQWLADH
                     LRTSGKLVLDAGAVKVLKSEGKSLLPIGVTAVEGYFERGDVVACVDVNGLEIARGLVN
                     YSASETARILKKPSSEIEKILGYVDEAELIHRDSLIVM"
     gene            1797..2294
                     /gene="purE"
                     /locus_tag="PKL42_04355"
     CDS             1797..2294
                     /gene="purE"
                     /locus_tag="PKL42_04355"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNU66339.1"
                     /translation="MSQKPLVAIIMGSDSDWPVMKNAAQMLADFGVAYEAKVVSAHRT
                     PDLMFKFAEDAKENGVQVIIAGAGGAAHLPGMVAAKTTLPVLGVPVPSKHLQGQDSLL
                     SIVQMPKGIPVATFAIGDAGAANAGLFAVSILANHDLSLAKKLDEFRQNQSDKVHAMS
                     LSEKP"
     gene            2350..3540
                     /locus_tag="PKL42_04360"
     CDS             2350..3540
                     /locus_tag="PKL42_04360"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HNU66340.1"
                     /translation="MSHSPIKQEHIILPPAMLGMLGGGQLGRFFVIAAHEMGYQVTVL
                     DPDKNSPAGKIADVHLCANYDDADALQEIAKTCAAVTTEFENVPASTLQKLAESVVVR
                     PSADSVAIAQHRVLEKNFIKDAGLPVAPFAVINEAADLPDDTSTLYPAILKVARFGYD
                     GKGQARVKNQSEAQLAFAEFKHEVCVLEQMLPLDLEVSVVLARDANGHIEAFTVAENS
                     HLNGILDISIAPARCSEVVKANARELAKKLAEKLSYVGVLGVEFFLVGKQLLVNEIAP
                     RPHNSGHYTIDACVTNQFEQQVRVLTGLPLGDASLHSNAVMVNILGDSWFSNTESARE
                     KKEPLEPAWNKACSHANLKLHLYGKAEPRIARKMGHFTVIGKDVKSVLNAALVARSEL
                     HIGE"
     gene            complement(3645..4037)
                     /gene="rpsT"
                     /locus_tag="PKL42_04365"
     CDS             complement(3645..4037)
                     /gene="rpsT"
                     /locus_tag="PKL42_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNU66341.1"
                     /translation="MANTAQARKRARQSVKVNAHNAALRSTLRTAIKKVIKAIEAGDK
                     AAAVVSYQENVSIIDRIADKKIIHKNKAARHKSRLTAAIKALKGDAPATLTKKAAPKA
                     KAAPKAKAEAKPKAAAPKKAAVKKAKAE"
     gene            4219..5760
                     /gene="murJ"
                     /locus_tag="PKL42_04370"
     CDS             4219..5760
                     /gene="murJ"
                     /locus_tag="PKL42_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNU66342.1"
                     /translation="MNLLKVLAKVGSMTFVSRILGFVRDTLIARAFGASMLSDAFIVA
                     FKIPNLLRRISAEGAFSQAFVPILGEYKSQRSFDETHQLVSRVGSWLGLILVVVTLLG
                     MLAAPFIVTLIAPGFRTDAGKMQLTIELLRITFPYIFFISLVSMAGGVLNTYNKFSIP
                     AFTPVWLNVSMIAAVLFFADYFDEPIKVLAWAVFVGGALQLAFQIPFLKQIGLLPRLD
                     FTRDEGVWRILKLMGPAVLGVSVAQISLILNTIFVSFLKTGSVSWLYYADRLMEFPTG
                     VLGVALGTILLPSLSKAFASKDESEYQQLMDWGLRLTFLLAAPAAVALAILATPLVCA
                     LFFYGKFTANDVMMTEQALIAYSVGLLGLILVKILAPGFYARQNVKTPVKIAIFTLVL
                     TQVMNVLFVFVLDLRHAGLALAIGLGACANASLLYYYLRQSGVFQPQAGWLAFLFKLA
                     AALAVMGFVLYFAMGETASWLSYTVLERIGYLAGLVLLGAASYFAALYMLGFRPRDYV
                     RRVNR"
     gene            5760..6653
                     /locus_tag="PKL42_04375"
     CDS             5760..6653
                     /locus_tag="PKL42_04375"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNU66343.1"
                     /translation="MQVFRHFQANYRAPLALAIGNFDGLHLGHQALLTRLVNEAKLQN
                     VTSAVLTFEPHPREFFTPESAPARLCSMREKLEHFAAANVERVHICRFDKRFASLSSQ
                     DFMRMILRDALNAQTILVGEDFRFGAKRVGSIVDIRQAGFGLISLPQVNLAGARVSST
                     RVRSALATGDLAQAAQLLGRPYSISGKVVHGAKRGRQLGFPTANVHMRHERPPMTGVY
                     AVKLDGMNAVANLGVRPTIAGVPKVSLEVHVLDYQADLYYKHVHVEFLLKIRDEMKFD
                     GLDALKAQIAADVTVAREFFM"
     gene            6699..9533
                     /gene="ileS"
                     /locus_tag="PKL42_04380"
     CDS             6699..9533
                     /gene="ileS"
                     /locus_tag="PKL42_04380"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNU66344.1"
                     /translation="MTEKNSEENKDTKYKLNLPETTFPMRGDLAKREPAWLKAWNDKK
                     LYQRIRASRKGAKKFILHDGPPYANGDIHIGHAVNKILKDIIIKAKTMDGFDAPYVPG
                     WDCHGLPIELVVEKNHGKNIAPAKFRELCRAYAAEQVEKQKKDFIRLGVLGDWDNPYL
                     TMDFKTEADIMRALGDIYKNGYLYQGSKPVHWCVDCGSALAEAEVEYEDVNSPAIDVG
                     FKIVDNKALSKAFGVEVNGDAYAVIWTTTPWTLPANQAVSVNAELEYDLIQTSKGLLI
                     LVHELAKATLARYGETDTQLIASCKGDALKGLALQHPFDNRQVPIICGDHVTTDAGTG
                     LVHTAAAHGNDDWLVMRANFPNEKPRVLMGGDGKFFNSELVEFEAIRGLDRKEANKVI
                     LAKMQENGTLLASARLNHSFPHCWRHKTPLMQLATHQWFIGMYAQDAVGISLREYANK
                     AVDATEFFPSWGRARLEAMIKNRPDWCVSRQRNWGVPMPFFVHKETGEPHPQTAALLE
                     QAALLTEQQGVEAWFSLDGDAFLAQHAPANADQYKKVTDTLDVWFDSGTTHAAVLKRR
                     TELAFPADLYLEGSDQHRGWFQSSLLTGCAIDGRAPYDALLTHGFVVDGSGHKMSKSK
                     GNVVAPQKVMDTYGADILRLWVASTDYSGELTISDEILKRVADSYRKLRNTMKFLLAN
                     LADFDASKDLLPVNDWMEIDRYALHLTSQLQTQVLADYSRYEFHLAVQKLVAFCSDDL
                     GSFYLDILKDRLYTAGENSPARRAAQSALHHITHTFMRLVAPILSFTADEIWQSLNLS
                     AEVTVFEDVWYSIPRHGLTDERLQAWEEILRVRSFVAKQIEMLRTEGKVGSSLQAELE
                     FHVPAATYNALASLEDDLRFVMITSKAELVSKTNDSTIMNVSVTPSAHQKCDRCWHYR
                     ADVGADKAHPHICGRCVSNLFGKGEARKYA"
     gene            9526..9984
                     /gene="lspA"
                     /locus_tag="PKL42_04385"
     CDS             9526..9984
                     /gene="lspA"
                     /locus_tag="PKL42_04385"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNU66345.1"
                     /translation="MRKYFGLSAVIVALDLYTKHLIEQDFQFGEKLTFTSFFDLVRYH
                     NEGSAFGFLNDAGGWQKWFFAVISVVAVIVMIYLIRKHQTEKLFCWGLALVLGGAIGN
                     LYDRVTLGYVVDFLNFHINDYYWPAFNVADSAIVGGVGLLLLDSFKKPNN"
     gene            10003..10659
                     /locus_tag="PKL42_04390"
     CDS             10003..10659
                     /locus_tag="PKL42_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HNU66346.1"
                     /translation="MPDWKKLIEGKISLAKRGAKPSKASENGKTSENARIPAGQKQVN
                     NFPILDLGIKPEIKLDDWRLRVFGLVENEIKLDWAQFQALPQITDISDFHCVTRWSQL
                     DMDWQGVRVRDVLALAKPLNTAHFATMHSYDGYTTNLPIEALLDDDVILAHAVFGKAL
                     SREHGGPVRMIVPKRYAWKGAKWIKAIELHEHDKRGFWEVRGYHNEADPWLEQRFSED
                     "
     gene            10770..11087
                     /locus_tag="PKL42_04395"
     CDS             10770..11087
                     /locus_tag="PKL42_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HNU66347.1"
                     /translation="MVDLSFTFSGECWLWTAENQVSWHFVTLPQDQSEEIKFFNQNLH
                     GKRRGWGSVRVAVTIGNTHWQTSIFPSSQLNAYLLPIKSEVRKSEKIAVGDSVQLALK
                     INV"
     gene            complement(11254..12174)
                     /locus_tag="PKL42_04400"
     CDS             complement(11254..12174)
                     /locus_tag="PKL42_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HNU66348.1"
                     /translation="MAAGSLLALLDDIATMLDDVAVMTKVAAKKTAGVLGDDLALNAQ
                     QVTGVDASREIPVVWAVAKGSFINKLILVPAALAISYFIPWLITPLLMIGGAYLCFEG
                     FEKIAHKLLHSKHEDKAHENELAQAVADPKVDLVALEKDKIKGAIRTDFILSAEIIVI
                     ALGTVATATFSKQVAVVSLIALVMTVGVYGLVAGIVKLDDLGLYMMMRKGKSFFKQVV
                     RKVGTWLLNLTPYLMRTLSILGTAAMFLVGGSIIGHGIPALHHLTEPMRNISALLPIM
                     FDGVLGLIVGAICVALFTIYTKIMSQKQPA"
     gene            complement(12245..12808)
                     /locus_tag="PKL42_04405"
     CDS             complement(12245..12808)
                     /locus_tag="PKL42_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNU66349.1"
                     /translation="MNSLPSHAPQSEQPSLLLVDDDEAFLGVLAKAMRKRGFAVSTAN
                     SAESAFELAQLDAPEFAVVDLKMSGNSGLVLVRQLASLNAGTRIVVLTGFASVATAVE
                     AIKLGATHYLAKPVDADEIIAAFGKQSGDANMELTSNPLTIDRLEWEHLQRVLAECGG
                     NVSETARRLNMHRRTLQRKLSKNPTKP"
BASE COUNT         3374 a         2593 c         3276 g         3690 t
ORIGIN      
        1 agggtggttt gggcaacgta cactttaaat ccagtttaaa tcgtgcgccg cgccaatgca
       61 ccaaaggtga actcggtgag gaatttgagc tttatttaga actgaaggtg ttggctgatg
      121 ttggcttgct tggcatgcca aatgccggta aatcaacctt tatccgctca gtttctgctg
      181 caaaaccgaa agtagccgac tatcctttta ctaccttgca ccctaattta ggcgtagtgc
      241 gggtagatgc tgaacgtagt tttgtgattg cggatatccc cggcattatt gagggtgccg
      301 ctgaaggtgc aggccttggg catcaattct tacgccattt gcagcgcacc tctttattgt
      361 tgcatttggt cgatattgca ccatttgatg aggccgttga tccggtgcat gaggctaagg
      421 cgattgttga agagcttaga aagtatgatg aagccctcta tgaaaaaccg cgctggttag
      481 tgctaaataa aatcgacatg ctgcaagatt cagagcaagc agtaaaacag tttgtcaaag
      541 actatggctg gaaaggccgt gtgtttgcga tatccgccat tagcggtatt ggttgtaaag
      601 aactcaccta tgccatcatg caacacatag atgacagcaa agcgcaagac gctcaagagt
      661 tggaaaatga aatctctggt acatgatgca aaagtgattg tcgttaaagt aggctctagc
      721 ctcgtgacga ataatggcaa cggcttagac cacgctgcca ttgtcgcgtg ggcagcacaa
      781 attgccagta ttgtgaagca aggtaaacag gtggtcttag tttcaagtgg tgcagtggcc
      841 gaaggcatgc aacgattagg ttggaaaaag cgtccaactg cggtgaatga actacaagcg
      901 gcggcggccg tcgggcaaat gggtttagtg caaatgtacg agcgctgctt tgccgaacat
      961 gatttacaca ccgcacaggt attgctcacg cacgatgatt tggcggatag aaagcggtat
     1021 cttaatgcac gcagtacgtt acgtactttg ctggatttaa aggtgattcc tatcatcaac
     1081 gaaaacgata cggtcgtcac cgatgaaatt cggtttggtg ataatgatac attaggtgcg
     1141 ctggtggcga atttaattga agcggatgca ctggtgattc ttaccgatca ggctggctta
     1201 tatactgcgg acccacgtag caacccaaaa gctgagtttg ttcagcacgc cactgcgggt
     1261 gatgttgcat tagaacaaat ggcgggtggc gcaggcagta gtgttggcac cggtggtatg
     1321 ctgactaaaa ttctcgcggc aaagcgtgca gcgcgcagtg gcgcacatac ggtgatcgct
     1381 tcaggtcggg aaaaagaagt attgttgcgt ctagcaaatg gtgaagtcat tggaacgcat
     1441 ttagaggcta cgcaaattaa aacggttgca cgtaaacaat ggctagcaga ccatttacgc
     1501 acttcaggca aactggtgtt agatgcgggt gccgtaaaag tattgaagtc cgaaggcaaa
     1561 agtttattgc ctattggggt gactgccgtc gaaggctatt ttgagcgtgg tgatgttgtc
     1621 gcttgtgttg atgtgaatgg acttgaaatc gcgcgcggtc tagtcaatta ttcggcgagc
     1681 gagaccgcgc gtattttaaa aaaacccagc agtgaaattg agaaaatact aggctatgtc
     1741 gatgaagctg aattaattca tcgcgatagt ttaatcgtga tgtagaagga tacagaatga
     1801 gtcaaaaacc attagtggca attattatgg ggtcggatag tgattggccc gtgatgaaaa
     1861 atgcagcaca aatgctggca gattttggtg tggcctacga ggccaaggtg gtttcagcgc
     1921 accgcacgcc agatttaatg tttaagtttg cagaagacgc gaaagaaaat ggcgttcaag
     1981 ttattatcgc aggtgctggt ggcgcagcac atttacctgg catggtggct gctaaaacga
     2041 ccttgccagt attgggcgtt cctgtgccaa gcaaacattt acaaggtcaa gattcattat
     2101 tgtccatcgt gcaaatgccc aaaggtattc cagtcgctac ctttgcgatt ggtgatgcag
     2161 gggcagcgaa tgctggtttg tttgctgttt caatattagc gaatcatgac ctatctctgg
     2221 ccaaaaaact agatgaattc cgccaaaatc aatctgataa agtgcacgca atgtcgttaa
     2281 gcgagaagcc ttaatctaca attgtttgaa taatttgatt atagtaaaaa tttacaaagt
     2341 gttgaaccaa tgagtcattc acctataaaa caagaacata tcattcttcc accagccatg
     2401 ctgggcatgc ttggcggtgg acaattgggc cgtttctttg tgatagcagc gcatgagatg
     2461 ggttatcagg taacggtgct ggaccctgat aaaaatagcc cagctggcaa gattgccgat
     2521 gtgcatttat gtgcaaatta cgatgatgca gacgcgcttc aagaaattgc aaaaacctgt
     2581 gctgcggtga ccactgagtt tgagaatgtt cctgccagca cgttgcaaaa actagcagag
     2641 tcagttgttg tgcgaccaag tgccgatagt gttgcgattg cgcaacaccg tgtgctggag
     2701 aaaaatttca ttaaagatgc tggcttgcct gttgcgccgt ttgcagtcat taacgaagcc
     2761 gccgatttgc cagatgacac cagtacactt tacccagcaa tattaaaagt agcgcgtttt
     2821 ggctatgatg gaaaaggaca ggcgcgcgta aaaaatcaaa gcgaagcgca gttggcattt
     2881 gctgaattta agcatgaagt ttgtgtgttg gagcaaatgc taccgttaga tttagaagtt
     2941 tcagtcgtgc ttgcgcgcga tgcaaacggg catattgaag ctttcactgt tgctgaaaac
     3001 agccatttaa atggcatttt agatattagt attgcgcccg cccgatgcag tgaagttgtt
     3061 aaagccaacg cacgtgagtt agcaaaaaag cttgctgaaa aactcagtta tgttggagtt
     3121 ttgggggtgg agttttttct agtaggtaag caattgctcg tcaatgaaat tgccccgcgc
     3181 ccacataatt ctggccatta cacgatagat gcctgcgtga cgaaccaatt tgagcagcaa
     3241 gtgcgagtac ttacgggttt gcctttgggc gatgctagtt tacacagcaa tgcggtgatg
     3301 gtgaatatat tgggagatag ttggttttct aatacagaaa gtgcgcgaga gaaaaaagaa
     3361 cctctggaac ccgcatggaa taaggcttgc agccatgcta acttgaagtt gcacctttat
     3421 ggcaaagctg aaccaagaat cgcgcgcaaa atgggacact ttactgtgat tggaaaagat
     3481 gtaaaatcgg tgctaaatgc agcgcttgtg gcgcgcagtg aactgcatat tggcgagtaa
     3541 atttaaacga ggcttgagaa gtcactaaca cgctatgaat aatcaataaa aaagccccgc
     3601 atattttgcg gggctttttt tattgttgct gttttgttgc gaaactattc agcttttgct
     3661 ttttttaccg ctgctttttt tggtgcagct gcttttggtt ttgcttcagc tttggccttt
     3721 ggcgctgctt tagctttagg cgcggctttt tttgttaatg ttgcaggtgc gtcgcctttt
     3781 aaggctttaa ttgctgccgt taaacggctt ttgtgacgag ctgctttatt tttatggata
     3841 atctttttgt cagcaatacg atcaataatg ctaacgtttt cttgatatga aacaactgca
     3901 gcagctttgt cgccagcctc aatcgcttta atcacttttt taattgcagt acgcaaagta
     3961 gagcgcaacg ctgcattatg tgcgtttact tttacggatt gacgcgcgcg tttgcgagct
     4021 tgtgcggtat ttgccatgtg taatttatcc taatgcttaa cgggtgttaa tacgaaaccg
     4081 tgcatattag tgttttttgt ctgatttggc aacaactaat caccattttt ctataaattt
     4141 atgcttaatt gatatggcaa aaaatagtct catctgcgtg ttaaaatacg cgattatttc
     4201 gaaatcaccg tgtattcaat gaatttactc aaggttcttg ccaaagtagg tagcatgacg
     4261 tttgtgtcac gcattttggg ctttgtgcga gatacactca ttgcacgtgc ctttggcgca
     4321 agtatgttga gtgatgcatt tatcgtcgcc tttaaaatac caaacttatt gcgcagaatt
     4381 tctgctgaag gcgcatttag tcaggctttt gtgccaattc taggtgaata taaaagccag
     4441 cgtagctttg atgaaacgca ccaattggtg agccgtgttg gtagttggtt gggattgatt
     4501 ttagtggtgg ttacattatt aggtatgttg gctgcgcctt ttatagtgac gcttattgcc
     4561 cctggtttta gaactgacgc aggtaaaatg cagctcacca tcgaattgct gcgtattact
     4621 tttccgtata ttttctttat ctcgctagtc tctatggcgg gaggcgtatt aaacacttat
     4681 aacaaattta gtattccagc atttacgccc gtgtggctca atgtctcgat gattgctgca
     4741 gtattgtttt ttgccgatta ttttgatgag cccattaagg tgctggcatg ggcagtgttt
     4801 gtgggcgggg cattgcagtt agcttttcaa attccatttt taaaacaaat tgggttatta
     4861 ccaaggcttg attttacccg tgatgaaggc gtatggcgga tattgaaatt aatgggcccg
     4921 gctgtgctag gggtttctgt ggcgcagatt tcactgattc taaatacgat ttttgtatct
     4981 tttttaaaaa caggcagtgt cagttggctt tattatgcag accgtctcat ggagtttccc
     5041 acgggcgtgc tgggtgtggc gttagggact attttattgc ccagtttatc taaggcgttt
     5101 gccagcaaag atgaaagcga ataccagcaa ctaatggatt gggggttgcg cctaacattt
     5161 ttattggctg ctcctgcagc tgtagcttta gcaatattag caacgcctct tgtatgtgcg
     5221 ctttttttct acggaaaatt tactgcaaac gatgtaatga tgaccgagca ggctttaatt
     5281 gcctatagcg taggcttgct gggtttgatt ttggttaaaa ttttggcgcc tggtttttat
     5341 gcacggcaaa atgtgaaaac gccagtgaaa attgcgattt tcactttagt gttaacccaa
     5401 gtcatgaatg tattgtttgt atttgtactc gatttacgtc atgcaggctt ggcattggcg
     5461 atagggcttg gtgcatgtgc aaatgccagc ttgctttatt attatttacg ccaatcaggc
     5521 gtatttcaac ctcaggctgg ctggctggct ttcttgttca agttagccgc agccttggcc
     5581 gtgatgggat ttgtgctgta ttttgcaatg ggagaaacag catcttggct tagctatact
     5641 gtgttagagc gaatcggcta tttggctggt ttggtattgt taggtgcagc cagctatttt
     5701 gccgcactat atatgcttgg ttttagacca agagattatg tgcggcgggt taatcgctaa
     5761 tgcaggtctt taggcatttt caggccaatt atcgtgcgcc cttagcgtta gccattggta
     5821 attttgatgg gttacatttg ggccatcaag ctttgttaac gcgtttggtt aatgaggcaa
     5881 agttgcagaa tgtcacttct gccgttttga cgtttgaacc tcacccacgc gagttcttta
     5941 cgccagaaag tgccccagca aggctttgct caatgcgtga aaagcttgag cactttgccg
     6001 ccgcaaatgt agagcgtgtg catatatgcc gctttgataa gcgctttgcg tcattgtcct
     6061 cacaagattt tatgcggatg attttacgag acgctttgaa cgcacaaacg atattagtgg
     6121 gtgaagattt tcgttttggt gcaaaacgtg tgggttctat cgttgatatc aggcaagcag
     6181 gatttgggct tattagtttg ccgcaagtga atttggcggg tgcacgtgtt tcaagtacgc
     6241 gagtacgcag tgctttggct acgggagact tagcccaagc ggcgcagttg ctgggccggc
     6301 cttatagtat cagtggtaaa gtggttcacg gggcaaaacg tggcaggcaa cttgggtttc
     6361 ctactgcaaa tgtgcatatg cggcacgagc ggccgccaat gacgggtgtt tatgcggtaa
     6421 aattggacgg gatgaacgct gtggctaact tgggggtacg gcccaccatt gcaggcgtac
     6481 caaaagtgtc actcgaagtc catgtgcttg attatcaagc ggacttgtat tacaagcatg
     6541 tgcatgtaga gtttttactt aaaattcgtg atgaaatgaa gtttgatggc ttagatgcgc
     6601 taaaagcgca aattgcggcg gacgtgaccg ttgcaagaga attttttatg taggactgca
     6661 attgattgcg ctcctacaaa aattagctgg aataaaaaat gacagaaaaa aactcagaag
     6721 aaaacaaaga tacgaaatat aaattaaacc tacctgaaac cacgttcccc atgcgtggtg
     6781 atttagccaa gcgcgaaccc gcatggttaa aggcttggaa tgataaaaag ctttatcagc
     6841 gcattcgtgc atcgcgcaaa ggtgcaaaaa aattcatttt gcatgatggc ccgccgtatg
     6901 ccaatggcga tattcacatt ggccatgccg tcaataaaat tttaaaagac atcattatta
     6961 aagccaaaac catggatggc tttgatgcgc cttatgtgcc gggctgggat tgccacggct
     7021 tgccgattga gttggtggtg gaaaaaaatc acggtaaaaa tattgcccct gcaaaatttc
     7081 gcgagttatg ccgcgcttat gccgctgaac aagtggaaaa acaaaagaaa gactttattc
     7141 gtttaggtgt gttgggtgat tgggataacc catatttgac gatggatttt aaaaccgaag
     7201 ccgacattat gcgtgctttg ggtgacattt ataaaaacgg ctatttatat caaggtagca
     7261 agcctgtgca ttggtgtgtc gattgcgggt cggcgttagc tgaggctgaa gttgaatatg
     7321 aagatgttaa ttcgcctgcg attgatgttg ggtttaagat tgttgataac aaagcgttaa
     7381 gcaaggcttt cggtgtagag gttaatggtg atgcttatgc tgtgatttgg acaacgaccc
     7441 cttggacgtt gccagccaac caagcagtga gtgtcaatgc tgaacttgaa tacgatttaa
     7501 tacaaaccag caaaggcttg ctgattttag ttcatgagct agctaaagca acattagcac
     7561 gctatggcga aactgatact caattgatag cttcttgcaa aggcgatgct ttaaaaggtc
     7621 tggctttgca acacccattc gacaaccgcc aagtgcctat tatctgtggg gaccacgtca
     7681 cgaccgatgc gggtacaggc ttggtgcata ccgccgccgc gcatggtaat gatgactggt
     7741 tggtcatgcg cgctaacttc cccaatgaaa agccacgtgt gctgatgggt ggtgatggca
     7801 agttctttaa cagtgaatta gttgagtttg aggcaatacg cggcttggac cgcaaagaag
     7861 ccaataaagt gattttggct aaaatgcaag aaaatggcac attgttagct tctgcgcgtt
     7921 taaaccatag ctttccgcat tgctggcgcc ataaaacgcc attgatgcaa ctggcgacac
     7981 atcaatggtt tattggcatg tatgcgcaag acgcggtggg cattagcttg cgcgagtatg
     8041 cgaataaagc tgtagatgca actgagtttt tcccgtcatg ggggagagca cgtttagaag
     8101 ccatgattaa aaaccgtcca gattggtgcg tttcacgcca gcgtaactgg ggcgtgccga
     8161 tgccgttttt tgtgcataaa gaaacaggtg agccgcatcc acaaacagct gcgttattag
     8221 agcaagccgc gttgctgacg gagcaacagg gtgttgaagc ttggtttagt ttagatggcg
     8281 acgcctttct tgcgcaacac gcacctgcaa atgctgacca atacaaaaaa gtgaccgaca
     8341 ctttagatgt ttggtttgat tcaggcacca cgcatgcggc cgttttaaaa cgtcgcactg
     8401 agttagcttt tcctgctgat ttgtatttag aaggttcaga tcagcatcgc ggctggtttc
     8461 aatccagttt gctgacaggc tgtgccattg atggacgtgc gccttatgat gccttgctca
     8521 cgcacggttt tgtggtggac ggtagtggcc ataaaatgag taaatctaaa ggcaacgtgg
     8581 ttgcgccaca aaaagtgatg gacacttacg gcgcagacat tttgcgcctt tgggtagcca
     8641 gcaccgatta ttcaggtgaa ctgaccattt cagatgaaat tttaaaacgt gtggcagata
     8701 gttacagaaa attgcgcaac accatgaaat ttctgctggc caacttggca gattttgatg
     8761 caagcaaaga tttgctgcca gtgaatgatt ggatggaaat tgaccgttac gcgctacatc
     8821 taactagcca attgcaaacg caagtgttgg cggattattc acgttatgag tttcacttag
     8881 cggtgcaaaa attggtggca ttttgctcag atgatttagg cagtttttac ctcgatattt
     8941 tgaaagacag gctttatacc gcaggtgaaa attcgcccgc gcgccgtgcc gcgcaaagtg
     9001 ctttacacca tattacgcac acctttatgc gcttggttgc gccgatttta agctttaccg
     9061 ctgatgaaat ttggcaatca ctaaacttaa gtgcagaagt aacggtattt gaggatgtgt
     9121 ggtatagcat ccctaggcat gggcttacag atgagcgttt gcaggcatgg gaagagatac
     9181 taagggtgcg tagttttgtg gcaaaacaga ttgaaatgtt gcgcacagag ggtaaagtag
     9241 gttcgtcgct tcaagcagag ttggaatttc atgtgcctgc tgctacatat aatgcgctag
     9301 ccagcctaga agatgatttg cgttttgtga tgattacttc taaagcagaa ttggtttcta
     9361 aaaccaatga ttcaacgatt atgaatgttt cagtgacccc cagtgcgcac caaaaatgcg
     9421 accgttgctg gcactaccgc gccgatgtag gcgcagacaa agcacatccc cacatttgcg
     9481 ggcgttgcgt aagcaatttg tttggcaaag gcgaggcgcg caagtatgcg taagtatttt
     9541 ggcctgagtg ccgtgattgt tgcgctggat ttatacacta agcatttaat tgagcaggat
     9601 tttcagtttg gtgaaaagct gacttttacc tcattttttg atttggtgcg ttatcacaac
     9661 gaaggctcgg cttttggttt tttgaatgat gctggcggct ggcaaaagtg gttttttgca
     9721 gttatttctg tggtggcagt catcgtgatg atttacttaa tccgcaagca tcagaccgag
     9781 aaactttttt gttggggcct tgccctagtg ctgggtggcg caattggtaa tttgtatgac
     9841 cgcgttaccc ttggttatgt ggtggatttt ttgaattttc acatcaatga ttattattgg
     9901 cctgcgttta atgtggccga tagtgcgatt gtaggcggcg ttggtctgtt gttgttggac
     9961 agtttcaaaa agccgaataa ttagcgctta aagggtgatg ccatgccaga ctggaaaaaa
    10021 ctcatagaag gtaaaatttc actggcaaaa cgtggtgcaa agcccagtaa agccagtgaa
    10081 aatggtaaaa ccagtgaaaa tgcgcgcatt cctgctgggc aaaaacaggt taataacttt
    10141 cccattttag atttaggcat caagcctgag attaaattag acgactggcg actcagggtt
    10201 tttggcttgg tagaaaacga aatcaagctc gattgggcgc agtttcaagc cctgccgcaa
    10261 attaccgata tttctgattt tcattgtgtg acgcgctgga gccagctgga tatggattgg
    10321 caaggcgtgc gcgtgcgcga tgtgctggca ctcgccaaac cactgaacac cgcgcatttt
    10381 gccaccatgc acagctatga tggctacacc accaacttgc cgattgaagc tttgctggat
    10441 gatgatgtca ttctcgcgca tgctgtgttt ggcaaagccc tatcgcgcga gcacggcggc
    10501 ccagtgcgca tgattgtgcc caagcgctat gcgtggaaag gcgcaaaatg gattaaagcc
    10561 atagaattgc atgagcatga taaacgcggt ttttgggaag tgcgcggtta tcataatgag
    10621 gcagatccgt ggttagagca acgttttagc gaagattgaa tttaattcgc accaagcgtt
    10681 gttgcaaatg cttgccgtac aaatgcgtac tgtctgcgca tttgcgccta gcttgacgcg
    10741 tttaaattca atcttttctg gaggtttgag tggttgattt aagctttaca ttttcaggtg
    10801 aatgttggct gtggactgct gaaaatcaag tcagttggca ctttgtgacc ttgccgcaag
    10861 atcaatcaga agaaatcaag ttttttaacc aaaatctgca tggcaaaagg cgtggctggg
    10921 gttcggtgcg cgttgcggtg accatcggca acacgcactg gcaaacgtca atttttcctt
    10981 catctcaatt gaatgcgtat cttttgccta ttaagtcaga ggtgcgcaaa agtgaaaaaa
    11041 ttgctgtggg cgactccgtg caattggcgc ttaaaatcaa tgtgtaactc atgcatattt
    11101 agccagcaga aaaatacaaa tttgtgatta aaatttctac gagcaaaaaa atatgctccg
    11161 gaagccttgt aaatcaaggc tcgcagggca tcgaaaaagg ttgtgattaa atttgattaa
    11221 aaaataagca atttcttgct tgttctttta tgtttaagcc ggctgctttt gggacatgat
    11281 ttttgtataa attgtaaata gcgccacaca aatagcgcct acaattaagc ccagcacacc
    11341 atcaaacatg atgggtaata acgctgaaat attgcgcatg ggttctgtta agtgatgtag
    11401 cgctggaatg ccatgcccga taatgctacc acccaccaaa aacatggcgg ctgtacccaa
    11461 aatgctcaat gtgcgcatta aataaggcgt aaggttaagt agccaagtgc caactttgcg
    11521 cacaacctgc ttaaaaaagc ttttgccttt acgcatcatc atataaaggc ccaagtcatc
    11581 cagtttaaca atgcctgcca ctaagccata cacgcccacc gtcattacca aggcgatgag
    11641 tgaaacaacg gccacttgtt tgctaaaggt tgccgttgcc actgtaccta gggcaatgac
    11701 aataatttca gccgataaaa taaagtcggt gcgaatcgcg cctttgattt tgtctttttc
    11761 aagcgcgact aaatcgactt tggggtcggc aacggcttgt gctaattcat tttcgtgggc
    11821 tttatcttca tgtttgctgt gcagtaattt atgtgcgatt ttttcaaaac cttcaaaaca
    11881 taaatacgcg ccgcctatca tcagcaaagg cgtaatcaac catggaataa aatagctaat
    11941 ggccaatgcg gcaggtacta gaatgagttt attaatgaac gagccttttg ctaccgccca
    12001 aaccacgggg atttcgcgcg aggcatccac gcctgtcact tgttgcgcgt ttaaagctaa
    12061 gtcatcgccc agcacgccgg ccgttttttt tgcggctact ttggtcatca cagcaacgtc
    12121 atcaagcatg gttgcaatgt catctaatag tgcgagtaaa ctgccagcgg ccatgttttt
    12181 tcctgttagg tatttgtgaa attaaggtgg cattttaact taaaacgcac ctacttaaaa
    12241 acgattaagg ctttgtagga tttttgctta atttgcgctg caaagtgcgt ctgtgcatgt
    12301 ttaagcggcg cgctgtttca gaaacattac caccgcattc ggccagcacg cgttgtaaat
    12361 gctcccactc taagcggtcg atagtgagcg ggtttgaggt gagctccata ttggcgtcac
    12421 cactttgttt gccaaacgcg gcaataattt catcggcatc cacgggtttg gcaaggtagt
    12481 gtgtagcgcc gagtttaatg gcttcaactg cggtggcaac gcttgcaaac cctgtgagca
    12541 ccacaatgcg ggtgccagca tttaagcttg ccagttgccg caccagcacc aagccggaat
    12601 tgcctgacat tttaaggtca accacggcaa attctggcgc atccaattgg gcaagttcaa
    12661 aggcgctttc agcactgttg gccgtgctaa cggcaaaccc gcgtttgcgc atcgcttttg
    12721 ccagcacgcc taaaaatgct tcatcgtcat caaccaaaag taaacttggc tgttcgcttt
    12781 gtggcgcatg gcttggtaat gagttcatcg catcttggct ttcattttga aatgattagt
    12841 ttggcaagag ggaggcgtat ggtagataca acgccacctt ccgggtggtt agtaagtgtt
    12901 aactctccgt cataacgcgc cagcgtggtt tgg
//
LOCUS       DAOQSI010000044        12796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_3999_length_12796_cov_4.990817, whole genome shotgun sequence.
ACCESSION   DAOQSI010000044 DAOQSI010000000
VERSION     DAOQSI010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 12796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12796
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3999_length_12796_cov_4.990817"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..459)
                     /locus_tag="PKL42_04410"
     CDS             complement(34..459)
                     /locus_tag="PKL42_04410"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HNU66350.1"
                     /translation="MANTVHIDVVSAEASIFSGEAEFVVAPASGGEVGLYPNHAPLVT
                     TIKPGALRIKQANEAEETLIFISGGLLEVQPGIITVLADTAVRGHDLDEAKAIAAKEA
                     AEEALKNRTSDIDYASAQAELSEALAQIQTIERLRKSTH"
     gene            complement(501..1937)
                     /gene="atpD"
                     /locus_tag="PKL42_04415"
     CDS             complement(501..1937)
                     /gene="atpD"
                     /locus_tag="PKL42_04415"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNU66351.1"
                     /translation="MANANQVKIGKVVQCIGAVVDVEFPRDQMPKVFEALIIDDASSQ
                     AEAGLTLEVQQQLGDGIVRTIAMGSSDGLARGTAFKSTGDQITVPVGTGTLGRIMDVL
                     GRPIDEAGPIDSDERRSIHQKAPTFEELSPSVDLLETGIKVIDLVCPFAKGGKVGLFG
                     GAGVGKTVNMLELINNIAKQHSGLSVFAGVGERTREGNDFYHEMAEAGVIKLDDMKES
                     KVAMVFGQMNEPPGNRLRVALSGLTMAEKFRDEGRDVLFFVDNIYRYTLAGTEVSALL
                     GRMPSAVGYQPTLADEMGRLQERITSTKTGSITSIQAVYVPADDLTDPSPATTFQHLD
                     STVVLSRDIASLGIYPAVDPLDSTSRQLDPLVVGEEHYNVARSVQQTLQRYKELRDII
                     AILGMDELSPEDKLAVGRARKIQRFLSQPFHVAEVFTGAPGKYVPLKETIKGFKAIVA
                     GEYDHLPEQAFYMVGGIEEAVEKAKTLN"
     gene            complement(1995..2933)
                     /gene="atpG"
                     /locus_tag="PKL42_04420"
     CDS             complement(1995..2933)
                     /gene="atpG"
                     /locus_tag="PKL42_04420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HNU66352.1"
                     /translation="MAGSKEIRTKIKSVENTRKITKAMEMVAASKMRKAQDRMRASRP
                     YAEKIRNVAAHLSLAHSEYKHPFLVKREDVKNVGIIVVSSDKGLCGGLNTNVLRLTVN
                     QMKSWEAEGKKLSVSAIGNKGFSFMNRVGANVQSHITGLGDVPHLETLIGTIKVMLDA
                     YTSGQIDQLYICYTKFINTMKQEPVIEQLLPLTSERLGTHETTEKVEALTTSGVVKSA
                     LQIGAAKGHWDYIYEPEAKPVIDQLMVRYIESLVYNAVSENMASEQSSRMVAMKSASD
                     NAKNVIGELKLVYNKARQAAITKEISEIVGGAAAVS"
     gene            complement(2970..4511)
                     /gene="atpA"
                     /locus_tag="PKL42_04425"
     CDS             complement(2970..4511)
                     /gene="atpA"
                     /locus_tag="PKL42_04425"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNU66353.1"
                     /translation="MQLSTSEISELIKSRLENFSTSAEARTQGTVISVTDGIVRIHGL
                     SNVMAGEMIEFPGNTFGLALNLERDSVGAVVLGDYEHITEGDICKCTGRILEVPVGPE
                     LIGRVVNALGQPIDGKGPVNAKLTDKIEKVAPGVIARKSVSQPVQTGLKSVDSMVPVG
                     RGQRELIIGDRQTGKTAVAVDAIINQKGQNMTCIYVAIGQKASTIANVVRKLEENGAM
                     AYTIVVAAAASDSAALQFLAPYAGCTMGEYFRDRGEDALIVYDDLTKQAIAYRQISLL
                     LRRPPGREAYPGDVFYIHSRLLERAARVNEEYVEKFTEGKVKGKTGSLTALPVIETAA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     VRLALAQYRELAAFAQFASDLDEATRKQLDRGRMFTELMKQAQYAPLSVSNMAITLFA
                     ANKGYFDDVPTNKVLAFENKLHGFISSKYKAVAEAIETSKDLSADNEKALEAAIKDFK
                     ATTAY"
     gene            complement(4564..5100)
                     /locus_tag="PKL42_04430"
     CDS             complement(4564..5100)
                     /locus_tag="PKL42_04430"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HNU66354.1"
                     /translation="MAEISTIARPYAVAAYKLGKEQKALAKWSEMLGFATAVANNAQM
                     QAYIQDPKVVSSDLQAAFLKVCGEKLNENAQNLIKVLVEYGRLSILPAITSAFEELKA
                     QDEGVLDAQIIAAAKISDKEVKDLVKRLETKFGKKITASVTVDPEIIGGIKIVVGDTV
                     IDASVKGQLQNLAYALTA"
     gene            complement(5110..5580)
                     /locus_tag="PKL42_04435"
     CDS             complement(5110..5580)
                     /locus_tag="PKL42_04435"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNU66355.1"
                     /translation="MNINFTLIAQAIAFAVLIWFTVKFVWPPLLNAIETRQKEIADGL
                     AAASEGRSALEVAAKKSEVTINEAKQKASEIIGQAEKRASQIVEEAKGNAKVEGDRIL
                     AGAKAEIDQEVNRAKEGLRAQVSSLAIAGAEKILRKEIDAKAHSEMLSKLAAEL"
     gene            complement(5647..5913)
                     /gene="atpE"
                     /locus_tag="PKL42_04440"
     CDS             complement(5647..5913)
                     /gene="atpE"
                     /locus_tag="PKL42_04440"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HNU66356.1"
                     /translation="MDALALIQAYTGVGIGLIIGLGAAGACIGIGIMCASFLEGAARQ
                     PEMIPQLQGKVFLLLGLIDASFIIGVGLAMMFAFANPLLSVVAK"
     gene            complement(5984..6835)
                     /gene="atpB"
                     /locus_tag="PKL42_04445"
     CDS             complement(5984..6835)
                     /gene="atpB"
                     /locus_tag="PKL42_04445"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNU66357.1"
                     /translation="MATEHADNAHHTEQALTPSGYIEHHLSFNAHGVNGNVTDQGFWV
                     LHYDTLIMSVALGVLVMGLIWLVARKASAGVPTKAQAFVELVFGFIDDQVKNIFHGNR
                     HSFIAPTALTVFLWVFAMNSMDFLPVDWVAGIVSFFGGAHAKWRAVPTSDINTTFALA
                     LAIWFLMIYFSIKVKGLGGWVHELFCSPFGTKIWVWPANFLFNLIEYVSKPLSHSLRL
                     FGNMYAGEVIFLLLGLWAATGVSGTIFGAILGAGWSIFHILIVALQAFIFMMLTVVYL
                     AMAHESH"
     gene            complement(6873..7211)
                     /locus_tag="PKL42_04450"
     CDS             complement(6873..7211)
                     /locus_tag="PKL42_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HNU66358.1"
                     /translation="MLKIQLVATAVVALAAMLISGFNASVSSLLGGLTVVLGTTAAGL
                     IVKRGAATSEAASVLVNLLKAEAVKILVIVAVLAIIFNVYQQLVPYALIAGLAVALLL
                     SASALSKLKA"
     gene            complement(7441..8316)
                     /locus_tag="PKL42_04455"
     CDS             complement(7441..8316)
                     /locus_tag="PKL42_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNU66359.1"
                     /translation="MAKLKGLGRGLDALLAGDMGTVGEADSLMMLKVEQMQPGKYQPR
                     SYMDDAALQTLAASIKAQGIMQPILVRQLSDEQYEIIAGERRWRASQIAGLSEVPVLV
                     REIADEAALAMALIENIQRENLNPLEEALGIKRLIDEFAMTHEKAAEAVGRTRVTVSN
                     LLRLLTLSAPVQDMLMQSKLDMGHARALIGLTGAQQIMLAEKIIQDDLSVREVENLVK
                     KTMANIDENTEKNKSKQQVKYSVSKDVLRLQETLSDQLGASVSIKANANGAGVLKINY
                     ASLDQLDEILAKIAR"
     gene            complement(8309..9109)
                     /locus_tag="PKL42_04460"
     CDS             complement(8309..9109)
                     /locus_tag="PKL42_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNU66360.1"
                     /translation="MKILAITNQKGGVGKTTTCVNLAASLASLDKRVLLIDLDPQGNA
                     STGSGIDKAHLKHSIYHVLIGEKTLQDVVLRSEKGGYDVAPSNRDLAGAEVELVHEIA
                     REMRLKHAIAKLKDFYDFVLLDCPPALNLVTVNALTAANAVMIPMQCEYYALEGLSDL
                     VNTIKKVRAHLNPTLEIEGLLRTLFDHRNMLANQVSAQLISHFGNKVYKTIIPRNIRL
                     AEAPSYGLPVLNYDKNSKGAQAYIELAREIVSQSKQVLENIKKEVNHG"
     gene            complement(9106..9726)
                     /gene="rsmG"
                     /locus_tag="PKL42_04465"
     CDS             complement(9106..9726)
                     /gene="rsmG"
                     /locus_tag="PKL42_04465"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNU66361.1"
                     /translation="MTPDVVLTAGLAEMNIEVTPNAKQKLLAYLALLQKWNKVYNLTA
                     VRDPLDMVTLHLLDSLSVLPYIKTDNLLDVGSGGGLPGIVLAIMKPDLQVTTIDTVQK
                     KTIFMRQVKGELGLPNLEVVHARVEQFQPTEKFDAIITRAFSDIGLLIKLTQHLLAPH
                     GHWLAMKGVVPHHEFEGLNIRPKEIIPLKVAGLQAERHLVVIESNA"
     gene            complement(9723..11651)
                     /gene="mnmG"
                     /locus_tag="PKL42_04470"
     CDS             complement(9723..11651)
                     /gene="mnmG"
                     /locus_tag="PKL42_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNU66362.1"
                     /translation="MNYQENFDVIVVGGGHAGTEAALAAARMGQKTLLLTHNIETLGQ
                     MSCNPSIGGIGKGHLVKEVDALGGAMAAATDVGGIQFRILNSSKGPAVRATRAQADRI
                     LYKAAIRHKLENQPNLWLFQQAVDDIILEGERVVGVVTQIGLRFKAKSVVLTAGTFLG
                     GLVHVGMQNYSAGRAGDPPSISLAARLRELNLPAGRLKTGTPPRIDGRTIDYSVMTIQ
                     PGDNPVPVFSFLGNAAQHPTQLPCWIAHTNERTHEIIRSGLDRSPMYTGVIEGVGPRY
                     CPSVEDKIHRFADKESHQIFLEPEGLNTNEIYPNGISTSLPFDIQLALVRSVRGLENA
                     HILRPGYAIEYDYYDPRGLKSSLETKAIRGLFFAGQINGTTGYEEAAAQGLLAGANAA
                     LLAQEKEAWCPARDEAYLGVLVDDLVTNGVTEPYRMFTSRAEYRLQLREDNADMRLTA
                     KGRELGLVDDVRWEAFSRKQEAVSREQARLKKTFVQPANVSRETQLAVLGKEIDHEYS
                     LFELLRRPEVTYEALLTLGGLGQNNQNLAELAPEVREQVEIAAKYQGYIDRQADEVTR
                     SRGQENTKLPTDLDYREIHGLPIEAQQKLNAQKPETIGQASRISGITPAAISLLLVYL
                     KRKSRSKIIALKDSEKAA"
     gene            11969..12421
                     /locus_tag="PKL42_04475"
     CDS             11969..12421
                     /locus_tag="PKL42_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1924 domain-containing protein"
                     /protein_id="HNU66363.1"
                     /translation="MKKLNIILAAFLGLAALSTQAYAASNVTNAEVLTKKYTGIAQTV
                     NPEFVPSVSEGKTFYNRKFTLKDGKQAACASCHTSNPADAGKHIVTGKAIAPLAPAVN
                     KKRFRSLDKVEDKFTEHCNDILGADCTAGEKANYIVYLLTERTPTAKK"
BASE COUNT         3541 a         3100 c         2436 g         3719 t
ORIGIN      
        1 tgccttttta gtattgtaac cattgcactt atattagtgt gtacttttgc gtaagcgttc
       61 gatagtttgg atttgcgcaa gcgcctctga taactcagct tgcgcagagg catagtcaat
      121 atctgaagtt ctatttttaa gcgcctcttc agctgcctct tttgcagcaa tcgctttagc
      181 ttcatctaaa tcatggccgc gcaccgcagt atcagccaac actgtaatta tgccaggttg
      241 cacttccagc aatccgcctg atataaaaat gagcgtttct tctgcttcat tcgcttgctt
      301 gatacgcaag gcacctggtt taatggtagt tactaacggc gcgtgattcg ggtataaacc
      361 cacttcacca ccacttgcag gcgcgacaac aaactcagct tctcctgaaa atatgcttgc
      421 ttctgcactc accacatcaa tatgaacagt atttgccatt atttttcctt aaacatttaa
      481 ccaataacta ataacagaaa ttagttaagc gttttagctt tctcaacagc ttcttcaata
      541 ccacctacca tgtagaacgc ttgctctggc aaatgatcat actcaccagc aacaatcgcc
      601 ttaaagcctt tgattgtttc tttcaatggc acgtatttac caggtgcacc ggtaaacact
      661 tctgccacgt ggaatggttg tgacaagaaa cgttgaattt tacgtgcacg gcctactgct
      721 aatttatctt ctggtgacaa ctcgtccata cccaaaatag cgataatgtc gcgaagctct
      781 ttataacgtt gtaatgtttg ttgaacagaa cgtgcaacgt tgtaatgctc ttcaccaact
      841 accaatgggt ccaactgacg tgatgttgaa tcaagtgggt caaccgctgg gtagatacct
      901 aaagaagcaa tgtcacgtga tagaacaacg gttgagtcta aatgttgaaa tgttgttgca
      961 ggcgatgggt cagtcaagtc atctgctggt acataaaccg cttgaataga ggtaatagaa
     1021 ccagttttag ttgaggtaat acgttcttgt aaacggccca tttcatccgc cagtgtaggt
     1081 tggtaaccca cggctgaagg catacgtcct aacaacgcag atacttcagt accggccaat
     1141 gtgtaacgat agatgttatc aacgaagaac aacacgtcac gaccttcgtc acggaatttt
     1201 tcagccatgg tcaaacctga tagcgctaca cgtaaacgat tgcctggcgg ttcgttcatt
     1261 tgaccgaata ccatcgctac ttttgattct ttcatgtcat ctagtttaat cacgccagct
     1321 tcagccattt catggtagaa gtcgttacct tcacgcgtac gctcacccac acctgcaaac
     1381 acagataagc ctgagtgttg tttagcaatg ttattaatca attcaagcat gttcactgtt
     1441 ttgcccacac cagcaccacc gaacagaccc actttaccac ctttagcaaa tgggcaaacc
     1501 aagtcaatca ccttaatacc tgtttctaac aagtcaactg aaggtgacag ctcttcaaat
     1561 gttggtgctt tttggtgaat tgagcgacgc tcgtctgaat caattgggcc agcttcatca
     1621 attgggcggc caagcacatc cataatccgg cctagagtac ctgtacctac tggcacagta
     1681 atttggtcac cggtggattt aaacgcagta ccgcgcgcaa gaccatcaga tgatcccatc
     1741 gcgatggtac gcacaatgcc atcacctaat tgttgctgca cttctaatgt caagcccgct
     1801 tctgcttggc ttgatgcatc atcaataatc aatgcctcaa aaactttagg catttggtca
     1861 cgaggaaact caacgtcaac aaccgcacca atacattgca ctactttacc gatttttact
     1921 tgatttgcat ttgccatttt ctattcctaa cttcttttag tcagatttaa ttaaatactt
     1981 taataagttg caaactacga taccgcagct gcaccaccaa caatttcgga tatttctttt
     2041 gtaattgcgg cttgacgtgc tttgttatat accaatttaa gctcgccaat aacgttttta
     2101 gcattatcag atgcggattt catggcaacc atacgtgatg attgctctga ggccatattt
     2161 tctgatactg cgttatatac caatgattca atatagcgca ccatcaattg atcaatcaca
     2221 ggctttgctt caggttcgta aatgtaatcc caatggcctt ttgcagcgcc aatttgcagc
     2281 gcactcttaa ccacgccgct ggtcgttaac gcttcaacct tttctgtagt ttcatgagta
     2341 cctaagcgct cgctggtaag tggcaataat tgctcgatta ccggctcttg cttcatggtg
     2401 ttaataaatt ttgtatagca aatatacagc tggtcaatct gaccagaagt ataagcatca
     2461 agcatgactt taatcgtccc gatcagcgtt tctaaatgcg gaacatcgcc caaacccgtg
     2521 atgtgtgatt gcacatttgc acccacacga ttcataaaac taaagccttt gttgccaatc
     2581 gcgcttacgg acaatttttt gccttcggct tcccagcttt tcatctggtt tactgtcaaa
     2641 cgtaacacat tggtatttaa tccaccacaa agccctttgt cagagctgac gacaataatc
     2701 cctacatttt taacatcttc acgctttact aaaaaagggt gtttatattc tgaatgcgcg
     2761 agtgaaaggt gagccgctac gttacgtatt ttttcggcat aagggcggct ggcacgcatt
     2821 ctatcttgcg ctttgcgcat tttagaagcg gccaccattt ccattgcctt ggtgatctta
     2881 cgtgtatttt caacactttt gatcttggtt ctaatttctt tactaccagc catatgtttt
     2941 cctaaacagt attgtcttaa tagtcaaaat tagtatgctg tcgttgcttt aaagtcttta
     3001 attgcagctt ctaatgcttt ttcgttatct gcacttaaat ctttactggt ttcaattgct
     3061 tcagcaacag ctttgtattt tgaagaaata aaaccatgca atttattttc aaaagccaac
     3121 actttattcg ttggcacatc atcaaaatag cctttgtttg cggcaaacaa agtaattgcc
     3181 atgtttgaaa cacttaaagg tgcatattgt gcttgcttca ttagctcagt aaacatccga
     3241 ccacggtcca actgtttacg ggttgcttca tctaaatcag aagcaaactg ggcaaacgca
     3301 gccaattcac ggtattgcgc taaagctaaa cgtacaccgc cgcctaattt tttgatgact
     3361 ttagtttgtg cggcaccacc tacgcgagac actgaaatac cagcattaat tgcaggacga
     3421 ataccagcgt tgaacaagtc agtttccaag aagatctgac cgtccgtaat agaaattacg
     3481 ttggttggaa cgaatgcaga aacatcaccc gcagcggttt caatcacagg tagggctgtc
     3541 aatgaaccag ttttaccttt aaccttacct tctgtaaatt tctctacata ctcttcgtta
     3601 acacgagcag cacgctctaa tagacgtgag tggatgtaga acacgtcacc tgggtaagct
     3661 tcacggcctg gtggacggcg taataacaat gaaatttgac ggtaagcaat cgcttgtttt
     3721 gttaagtcat catacacaat taaagcatct tcaccgcggt cacggaagta ttcacccatc
     3781 gtgcaaccag cataaggtgc caagaactgt aaggcagcag aatcagatgc agcggcagcc
     3841 actacaatgg tatacgccat tgcaccattt tcttcaagct tacgcactac gttggcgatg
     3901 gttgaagctt tttgaccaat cgcaacatag atacaggtca tgttttgacc tttttgatta
     3961 ataattgcat ctaccgcaac ggctgtttta cctgtttggc ggtcaccaat aatcaactca
     4021 cgttggccgc gacccactgg caccatagag tcaacagatt tcaaacctgt ttgcactggt
     4081 tgtgacactg atttacgtgc aatcacacca ggcgcgactt tttcaatttt gtcagttagc
     4141 ttcgcattaa ctgggccttt accatcaatt ggctgaccta gcgcattgac tacacggcca
     4201 atcaattcag ggccaacggg cacttctaga atgcggcctg tgcatttaca tatatcacct
     4261 tcggtaatat gttcgtaatc gcccaaaacc actgcgccca ctgaatcgcg ctctaggtta
     4321 agtgctagac caaacgtatt gcctgggaat tcgatcatct cacctgccat cacatttgat
     4381 agcccgtgaa tacgaacgat accgtccgtc actgaaatga ctgtaccttg tgtacgcgct
     4441 tcagcacttg tagaaaaatt ttctaatctg cttttaatca gctcactgat ttctgatgta
     4501 gataactgca ttcctaaact cctataattt tttctgctta agcttaaagt aagcttaagc
     4561 gccttatgct gtaagtgcgt aggctagatt ttgcaactga cctttaacag aagcgtcgat
     4621 cacagtatcg ccaacaacaa ttttaatgcc gcctataatc tccgggtcta cagtaacgct
     4681 ggctgtaatt tttttaccga atttagtttc aaggcgcttc accaagtctt ttacttcttt
     4741 atcgctaatt tttgcagctg caataatctg agcgtccaac acaccctcat cttgcgcttt
     4801 caattcttca aaagcactcg taattgcagg caagatagac aagcgaccat attcaaccag
     4861 cactttaatc agattttgtg cattttcatt gagcttttcg ccgcatactt ttaaaaatgc
     4921 agcctgcaaa tcactactca ccactttagg atcttgaata taagcttgca tctgtgcatt
     4981 atttgcaact gcagtggcaa accctagcat ttcagaccac ttagcgagcg ctttctgctc
     5041 tttacctaat ttgtaagcag ctactgcgta gggtctagcg atggttgata tttccgccat
     5101 gtcttatcct taaagttctg ctgctagttt agaaagcatc tcgctatgcg cttttgcatc
     5161 aatttcttta cgtaagattt tttctgcacc tgcaatcgct aagctagaaa cctgagcacg
     5221 caaaccttct tttgcacggt ttacttcttg gtcaatttca gctttagcac ccgctaaaat
     5281 acggtcacct tcaaccttag cgttaccttt tgcctcttca actatttgac tcgcacgttt
     5341 ctcagcctga ccaatgattt cactggcttt ttgttttgct tcgttgatgg tcacttctga
     5401 tttctttgct gccacttcca aggcacttct gccttctgat gccgcagcca aaccatcagc
     5461 aatttctttt tggcgcgttt caattgcatt taacaacggc ggccatacaa atttcactgt
     5521 gaaccaaatc aacacagcaa atgcgatagc ttgtgcgata agtgtaaagt taatattcat
     5581 tacttactcc aatatttaag tcgtttcatt tgggctagag ttactagcaa ccaaacttga
     5641 ggctaattac ttagctacta cgcttaacaa tgggttagca aatgcaaaca tcatggctag
     5701 accaacacca ataataaatg aagcatcgat aagacccaat aacaagaata ctttaccttg
     5761 taactgagga atcatttctg gttgacgtgc tgcgccttct aagaagcttg cacacataat
     5821 accaataccg atacaagcac ctgcagcacc taaaccgata attaaaccaa taccaacgcc
     5881 tgtgtaggct tgaatcaacg ctaatgcatc cattttgtta ccctctctaa aattaaacta
     5941 aaactactta aacttacaac taaactacaa aacaaccaaa aatctaatgc gactcatgcg
     6001 ccatggccaa ataaactaca gtcaacatca tgaaaataaa ggcttgcaat gcaacaatta
     6061 gaatgtggaa gattgaccag ccagcaccta aaattgcacc aaaaatagtg cctgatacac
     6121 cggttgccgc ccaaaggccg agcaataaga aaatcacctc acccgcgtac atattgccga
     6181 ataaccgcaa tgagtgtgaa agcggttttg aaacatattc aatgagatta aataagaagt
     6241 tagctggcca tacccagatt tttgtgccaa aaggggagca aaacagctca tggacccagc
     6301 cgccgagacc tttaactttg atcgaaaaat aaatcattaa gaaccaaatt gccagtgcca
     6361 gcgcaaaggt tgtattaatg tcggatgtgg gaacagcgcg ccattttgca tgtgcgccac
     6421 caaaaaaaga aacaataccc gcaacccagt caacgggtaa aaagtccatg gagttcatgg
     6481 caaacaccca taaaaacacc gttaacgccg ttggagcaat aaagctatga cgatttccgt
     6541 gaaaaatatt ttttacttga tcatcaataa aaccaaacac caattcaaca aacgcttgcg
     6601 cctttgttgg tacacccgct gaagccttgc gagcaaccaa ccaaataagg cccattacca
     6661 acacgcccaa tgctaccgac atgattaacg tgtcgtaatg cagtacccag aaaccttgat
     6721 cggttacatt gccatttacg ccatgcgcat taaagctaag gtgatgttct atataaccag
     6781 aaggggttag cgcctgttcg gtatggtgtg cgttatctgc gtgttctgta gccataatct
     6841 aaatttactt taatattgtt aagttatctt gctcaagctt ttagtttgct caaggccgaa
     6901 gctgaaagta gtaacgctac agccaaacct gcaattagcg cataaggcac caattgttga
     6961 tacacattaa aaatgattgc caaaactgcg acaatcacca aaatttttac cgcttctgct
     7021 ttaagcaagt ttaccaacac cgaagccgcc tcacttgttg ccgcacctcg tttaacgata
     7081 agacccgctg ctgtcgtgcc taaaactact gttaagccac ccagcaacga ggaaacactt
     7141 gcattgaacc ctgaaatcaa cattgctgcc aatgcaacta ctgctgtagc caccaactga
     7201 attttaagca ttttgctaaa tacatctgat gtgttggatg ttgacaattg cttatgccca
     7261 gttaatttta atactgaaat tttttgctgt tttacgttct tttacattgt atttatagca
     7321 aagaccgcga ttttaaatta ttaccgcttt ttagtcaagg cttaagtgaa agtgtttttc
     7381 atatagttcg cacaaaatga accacttagc acaaatatcg attttgcaaa cttagcataa
     7441 ctagcgtgct atttttgcaa gaatctcatc cagctggtct aaacttgcgt aattgatttt
     7501 taatacgccc gcgccattgg cattggcttt gatgctcact gacgcaccca gttgatcgct
     7561 caacgtctct tgtagcctta atacatcttt gcttactgaa tatttaacct gttgttttga
     7621 tttgtttttt tcagtatttt catcgatatt agccatcgtt tttttgacta agttttcgac
     7681 ttcacgcacg gataaatcat cttgtataat tttttcagcc agcataattt gttgcgcgcc
     7741 tgttaaacca atcaaggcac gtgcatgccc catatctaac ttagactgca tcagcatgtc
     7801 ttgcactggc gcgctcaatg ttaacaagcg tagcaaatta gaaactgtaa cgcgtgtacg
     7861 gccaacggct tcagcggctt tttcatgcgt catggcaaat tcatctatta accgtttaat
     7921 gcctagcgcc tcttctaatg gatttaagtt ttctcgctga atattttcaa ttaaggccat
     7981 cgccagcgcg gcctcatcgg caatttcgcg cactaatact ggcacttcac tcaacccagc
     8041 tatttgactt gcgcgccaac ggcgctcacc tgcaataatt tcatattgtt catcagacaa
     8101 ttggcggacc aaaataggct gcataatgcc ttgtgcttta atggatgccg ccaaggtttg
     8161 tagggcggca tcatccatat aactacgcgg ctggtattta ccaggctgca tctgttcaac
     8221 tttaagcatc atgagtgaat cagcctcgcc aactgtaccc atatcgcccg ccaacagcgc
     8281 gtccaaccct ctgcccaacc cttttaactt agccatgatt tacctctttt ttgatgtttt
     8341 ctagaacctg tttgctttgg ctaacaatct cacgcgccag ctctatataa gcttgtgcgc
     8401 cttttgagtt tttatcgtaa ttgagtacag gcaagccata gcttggcgct tcggccaaac
     8461 gaatgttgcg cggaataatg gttttataca ccttgttgcc aaagtggcta attagctgcg
     8521 cggatacttg attcgccaac atatttcggt gatcaaataa ggtacgcaat aacccttcaa
     8581 tttccaacgt ggggtttaaa tgcgcgcgca cttttttgat ggtgttcact aaatctgaca
     8641 aaccttctag ggcgtaatat tcacactgca ttggaatcat cacagcattg gccgcggtaa
     8701 gtgcattcac cgtaactaaa ttgagggcag gggggcaatc gagcaataca aaatcataaa
     8761 agtcttttaa ttttgctatg gcatgcttta gacgcatttc acgggctatt tcatgcacta
     8821 attcaacttc agcgcccgcc aaatcacgat ttgaaggtgc aacgtcatag ccaccttttt
     8881 cgctacgcaa caccacatct tgcaatgttt tttcaccaat caatacatga taaatgctgt
     8941 gctttaaatg cgctttatca atgccgctac cggtgctggc gttgccttgt ggatcaaggt
     9001 caattaacag cacacgctta tctaagcttg ccaaacttgc tgccaagttt acacaagtgg
     9061 tggttttccc tacgccacct ttttgatttg tgattgctaa aattttcatg cattactctc
     9121 aataactacc aaatgtcgct cggcctgcaa acccgccact ttgagcggaa taatttcttt
     9181 aggcctgatg ttcaagcctt caaattcgtg atgcggcaca acgcctttca tcgcaagcca
     9241 atgcccgtgc ggcgctaata agtgctgggt aagtttgatc aataaaccaa tgtcagaaaa
     9301 agcgcgtgtg ataatcgcat caaacttttc tgttggctga aactgctcaa cccgcgcatg
     9361 cacaacctca agattaggta aacctagctc accctttact tgacgcataa aaatggtttt
     9421 tttttgcacg gtatcaatcg tcgtgacctg caaatccggc ttcataatcg ctagcacaat
     9481 gccaggcaac ccgccgccac taccaacatc aagcaaattg tctgttttaa tgtacggcaa
     9541 cacactcaaa ctatctaaca aatgcaacgt gaccatgtct agagggtcgc gcacagctgt
     9601 gaggttatac actttgttcc atttttgtaa taacgccaaa taggccagca atttctgctt
     9661 ggcatttggc gttacttcga tgttcatttc agccaagccc gctgttaata caacgtctgg
     9721 cgtcatgcgg ctttttctga atcttttaac gcaatgattt tactgcgcga tttacgcttt
     9781 aaatacacca gcaatagcga aattgccgca ggcgtaatgc ctgaaatacg gcttgcctga
     9841 ccaatcgttt ctggtttttg cgcgttgagt ttttgttgcg cctcaatcgg cagaccgtga
     9901 atttcacgat aatccaaatc agtcggtaac tttgtatttt cttggccccg actacgcgtc
     9961 acttcatcgg cctgacggtc gatataacct tgatattttg cagcaatttc aacttgctcg
    10021 cgcacttctg gtgcaagttc ggcaaggttt tgattatttt ggcctagccc acctaatgtt
    10081 agcaatgctt cataagtcac ctcagggcgg cgtagcaatt caaataagct atattcgtgg
    10141 tcaatttctt tacccagcac tgcaagctgt gtttcacgtg aaacatttgc aggctgcaca
    10201 aaggtttttt tcaagcgcgc ttgctcgcgt gacacggctt cctgtttgcg actaaacgcc
    10261 tcccaacgca cgtcgtccac caagcccaac tcacggcctt tggcggtaag tcgcatatcg
    10321 gcgttatctt ctcttagttg cagacgatat tctgcgcggc tggtaaacat gcgatacggc
    10381 tcggtaacgc cgttggtgac caaatcatcc accaaaacac ccaaataggc ctcatcgcgc
    10441 gcagggcacc aagcttcttt ttcttgcgcc aacagcgctg cgttagcgcc tgccaacagc
    10501 ccttgcgcgg cggcttcttc ataccccgtg gtgccgttaa tttgccccgc aaaaaataag
    10561 cctcgaatcg ctttggtttc caaactcgac tttaatccgc gcggatcgta ataatcgtac
    10621 tcaatggcat agcctgggcg caaaatgtgt gcattctcta agccacgcac cgagcgcacc
    10681 aacgctagct gaatatcaaa cggcaagctg gtagaaatgc cgtttgggta aatctcattg
    10741 gtgttcaggc cttctggctc taaaaatatt tggtgcgatt ctttatctgc aaaacggtga
    10801 attttatctt ccacacttgg gcaatagcgc gggcctaccc cttcaatcac gccggtatac
    10861 atgggcgagc ggtctaggcc gctacgaata atttcatggg tacgctcatt ggtatgcgca
    10921 atccagcagg gcagctgggt agggtgctgc gcggcgttac ctaaaaatga aaataccgga
    10981 accggattat cacccggctg aatggtcatc acagagtaat caatagtgcg gccatcaata
    11041 cgtggcggcg tgcccgtttt taacctacct gccggtaaat tcagttcgcg caaacgcgca
    11101 gccaatgaaa tagatggcgg gtcgcccgcg cggccagcgc tataattttg catacccaca
    11161 tgcactaagc cacccaaaaa cgtgcccgcg gtcagcacca cggactttgc cttaaaacgc
    11221 aagccgattt gcgtcactac accaaccacg cgctcgcctt ctaaaataat atcgtccacc
    11281 gcctgttgaa acagccacaa attgggttga ttttcaagct tatggcgaat cgctgcttta
    11341 tataaaatcc gatcagcctg cgcacgcgta gcgcgcactg ccgggccttt gctggaattt
    11401 aaaattctaa attgaatacc gccaacatcg gtggcagcag ccatagcgcc gcccagcgca
    11461 tccacctctt taaccaaatg acctttacca ataccaccga tgctcgggtt gcatgacatt
    11521 tggcccaaag tttcaatgtt gtgggtgagc aaaagtgttt tttgccccat gcgggccgct
    11581 gctagcgccg cttcggtgcc tgcatgaccg ccgcccacca cgatcacatc aaaattttct
    11641 tggtaattca tcattaaaat tcaggttttc aataaaatgc cgtaatgcgc aaagaagtga
    11701 aattttaacg caatctatag ctaaggtcat gcaaaacctt gatttaagtt aatgtttcac
    11761 gtgaaacata tttaattgag cgcacaacaa cactttattt acaaactttt catgctttct
    11821 taacaaaatt ggcataaaat tcgcttattg acatttgacc taccgctaat ttgttcggat
    11881 tgaatatgtt ataataatga aaaaattggc atattttttt aatatttaac tttaccattt
    11941 ttattaaata acttaaccgg agaaatacat gaaaaaatta aacatcattt tagctgcgtt
    12001 tttgggcttg gctgcactat caacacaagc ttacgctgcg agcaacgtca ccaatgctga
    12061 agtgctgact aaaaaataca ctgggattgc acaaactgta aaccctgagt ttgtaccttc
    12121 agtgtctgaa ggcaaaactt tttacaaccg taaatttaca cttaaagatg gcaaacaagc
    12181 tgcttgcgca tcatgccaca ccagcaaccc ggctgatgca ggtaagcaca ttgtgacagg
    12241 caaagcaatt gcgccgcttg ccccagcagt aaacaaaaaa cgtttcagaa gcttagacaa
    12301 ggttgaagat aaattcactg aacactgcaa cgacatctta ggtgcagatt gcactgcggg
    12361 tgaaaaagca aactacatcg tttatctttt aacagaaaga acgccaactg ctaaaaaata
    12421 gtgcctcgtt atttacgcat ttagttaagt tggtttttaa agcaatgcgc acgatgtaaa
    12481 acacatcaag tgcaaaaatg aaaaagcaat gtttcacgtg aaacattgct ttttttatgc
    12541 ccgttatttt gcttatttct agcgtaattt aacatgtaaa atttgcattt taagcaggtc
    12601 tttaatacac aaccttttac catgctctgg aagccttata aataaaggct tccagagcat
    12661 attttttctc tcggggataa tatgtttcta tcaaaaggtt ttaaccatat cctaggatca
    12721 taaaaagata gcgctgtttc ctattcagtc agcagaaact actttgtgtg ctatgcattt
    12781 ttggcgctta cggtct
//
LOCUS       DAOQSI010000045        12697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_4065_length_12697_cov_6.212466, whole genome shotgun sequence.
ACCESSION   DAOQSI010000045 DAOQSI010000000
VERSION     DAOQSI010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 12697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12697
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4065_length_12697_cov_6.212466"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="PKL42_04480"
     CDS             <1..524
                     /locus_tag="PKL42_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNU66364.1"
                     /translation="DSLGKQIASPLLNISEDPHIKKGLASSPFDNEGVATHARQLVKD
                     GVLQGYVLGSYSARKLGMQTTGNAGGNHNLIVQSSGQDFTALLKQMGTGLLVTELLGH
                     GMNMVTGDYSRGAAGFWVENGVILHPVEEITIASNMADMLKNIAAIGNDILVQGSKQV
                     GSILIERMTVASE"
     gene            complement(614..1423)
                     /locus_tag="PKL42_04485"
     CDS             complement(614..1423)
                     /locus_tag="PKL42_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HNU66365.1"
                     /translation="MRHPQKNYIKTTLAIASLCAVTILSGCATQANKDPLEGVNRAVY
                     KFNDVTDKAVFKPVATAYKAVTPSPIRTGFNNFFTNLGSITTVLNDLLQFKFAHAFTD
                     AGRFVINSTFGLAGFIDVAGKDNIALRKEDFGQTLGFWGVGNGAYLVLPFLGPSTLRD
                     TTGLVVDTTTTDPIHYTHNIGEIRLHNQLRLAQFIDKRTELLDASDLVDEASLDPYAF
                     MRDAYLQRRENQVNDNLAKRNLSNDEFFEEDDTLDNATTPATENQPAAETK"
     gene            complement(1617..2093)
                     /locus_tag="PKL42_04490"
     CDS             complement(1617..2093)
                     /locus_tag="PKL42_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66366.1"
                     /translation="MKWRIFVGLFVLFCGLAFWAGEADGKLFGNFLLFSIIPAVFFTL
                     FSAPTNLWGKVILGCVFVSSYSYSFYLGTKSYMRAYNECVTQGEVIREQLTTFYQQNL
                     QYPEHLSQINGFNACKRVMHPTILMYQKTTLGYQISFDDGHVLHQATESQPFEAHK"
     gene            complement(2090..2521)
                     /locus_tag="PKL42_04495"
     CDS             complement(2090..2521)
                     /locus_tag="PKL42_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66367.1"
                     /translation="MTHIEQQQNSSTTMQTPEKNAPKNLVPRSKAILISYCIMFAVAL
                     IFSIGAQFAIPLYETVFSSFGADLPWLTSIVVDYRKWLLLMPIYMSVIVIFFMVQSRV
                     TTEMNHAFKIIFFADLLTFIGIIATVVVAMYLPLFKMGAVA"
     gene            complement(2665..4092)
                     /locus_tag="PKL42_04500"
     CDS             complement(2665..4092)
                     /locus_tag="PKL42_04500"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNU66368.1"
                     /translation="MHYEKSLVWFRRDLRDYDHAALYHALKNSTQVYCVFVFDTEILN
                     ALKDKADRRVEFIWESVKVLKTALQAKGGDLMVLHGNAREEVPALALQLGVQAVFTNH
                     DYEPAAIARDLLVTQRLQPHQIALHHYKDQVIFEQEEVLSQVGKPYSVFTPYKNAWLK
                     KVNDFYLSPYPVDAYIQHLVKVEPTPLISLENLGFARTNLSAMRLPTGMHGGAQLFAD
                     FTERMQCYKEARDFPAVKGVSYLSVHLRFGTVSIRHLAREAWQRGGAGAETWLNELIW
                     RDFYFQILHHHPQVASGRAYKAEFESLNFPNHAEHFKAWCEGNTGYPLVDAAMRQLNQ
                     TGFMHNRLRMVAASFLVKDLLIDWRWGEQYFSEKLIDFDFSANNGGWQWAASTGCDAQ
                     PWFRIFNPITQSERFDSLGKFIRKYVPELGACDDKEIHAPWLIPPLRQQSLQLNLGKD
                     YPNPIVDHASQREKALALYKNTPTK"
     gene            complement(4094..4561)
                     /gene="rlmH"
                     /locus_tag="PKL42_04505"
     CDS             complement(4094..4561)
                     /gene="rlmH"
                     /locus_tag="PKL42_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNU66369.1"
                     /translation="MKLRIISVGHKMPAWVETACAEYIKRMPRELAIEIIEIKPDKRA
                     DGKNSVVVQEAEAKRILEAAGKDFLIACDERGQEVTTLLLAEKMQFWQGLGRDVSIVI
                     GGADGLHASLKQQADWLWGLSKLTLPHAFVRVLLCEQLYRAHSVNQGHPYHRE"
     gene            complement(4563..4799)
                     /locus_tag="PKL42_04510"
     CDS             complement(4563..4799)
                     /locus_tag="PKL42_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66370.1"
                     /translation="MPFSVEERAALLKLKGVGNTVISRLEEMGFASLNQLAQAKTQDI
                     LEYGANLTRATCWKNSPQARAAIDAVIQLAQSKA"
     gene            complement(4810..5199)
                     /gene="rsfS"
                     /locus_tag="PKL42_04515"
     CDS             complement(4810..5199)
                     /gene="rsfS"
                     /locus_tag="PKL42_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNU66371.1"
                     /translation="MTTNSNTPTSAPFDLEAMKTAVVDAIEDIKGFDISVMDVRKLTS
                     MTNYMIVASANSTRQCKAIADNVREKLKEKGFHIRGTEGEKEGEWVLVDLDDIVVHIM
                     VPTTRAYYNLEQLWGEAESRRGHIKAS"
     gene            complement(5200..5580)
                     /locus_tag="PKL42_04520"
     CDS             complement(5200..5580)
                     /locus_tag="PKL42_04520"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HNU66372.1"
                     /translation="MQRTMLKSKLHRVHTTHAELHYEGSCAIDEVLLEAANIREYEQI
                     HIYNVTNGERFSTYAIRGERNSGIISVNGAAAHKANPGDILIIASYADYHELELEKFK
                     PQLVYVDEKNRIQTQRNAIPAQAA"
     gene            5734..7548
                     /locus_tag="PKL42_04525"
     CDS             5734..7548
                     /locus_tag="PKL42_04525"
                     /inference="COORDINATES: protein motif:HMM:NF025067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HNU66373.1"
                     /translation="MTDLAPQNREHRFILSMALLQGVALLLIWLWVDTLSNARQYFNV
                     ILPLYVLAISFPLSLMLLAHQPRKLALSLAGAFSLIAAFCAMYFGDVSYVKGLPRYGV
                     EGLVFQFGFCMVMAWFIAVTFIEHFCQYGRWSNHYESLYDYSWRNAVKLITSGIFTGL
                     FWAALLLLAGLFKVLGITFFKTIIEDVYFIYPATAVAFGLGISLYNAKQDALSEFKRA
                     ILQVLGWLLPLVSLILLAFMITLPFKGLASLWGTGYATSLMLSLLALMVFLLNTAFQD
                     GKQTQYPAWLLKLSNLAVLTMPIYAMLCIYALYLRVNQYGWTDDRVWAASITLIMAIY
                     SVGYAFAAFQSFNQNTWMQAIKSVNVIAAVCLMAFIGLLYSPILSPMRIGVQSQLARL
                     HNETIAASQFDYEYLRFSGGKYGEAALKNLLADTTHPQAATIKPLVEATLKAQYRHYR
                     SVETKKPPVTLQTLQAHFNIYPKGTPIEAAFFERVLQEYQNHEVYFACLDFPQDTDCA
                     NTKPKDQLLAVDLNQDGHVEIVFFQRNGGGRAYKKEANSWIMFAHLNNYCCEAQETTE
                     TFQKNLQSQLEAGEVTATAPQWQDLKVGNKKYQLNVYR"
     gene            complement(7588..8451)
                     /gene="panC"
                     /locus_tag="PKL42_04530"
     CDS             complement(7588..8451)
                     /gene="panC"
                     /locus_tag="PKL42_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNU66374.1"
                     /translation="MQIVHTIAELRATLKNQSGISFVPTMGNLHAGHLQLVEIAKQHA
                     HCVVVSIFVNPLQFGANEDFGSYPRTLEADCEKLKAAGADIVFAPNVTEIYPDFDGKN
                     LNQTMTITPPPIASELCGASRPGHFSGVATVVMKLFNMVQPDVAVFGQKDFQQLFIIK
                     EMVKQFNLPIEIIAGDTMREASGLAMSSRNGYLNEAQRTQAAQLNAALQSMVQALKNG
                     DKNFSALENTATQQLNQQGWEVDYISIRSALTLQPAAAQDQHLVVLGAAKISNNQAGK
                     TRLIDNIEVHL"
     gene            complement(8666..9478)
                     /gene="panB"
                     /locus_tag="PKL42_04535"
     CDS             complement(8666..9478)
                     /gene="panB"
                     /locus_tag="PKL42_04535"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNU66375.1"
                     /translation="MSLTKLQTKVAAGEKIAMLTCYDTTFAKLMELAGVDMLLVGDSL
                     GMVLQGAENTLNVSMHHMTYHTKMVKNGAPNTVIMADMPIGSYEQDNEAAYKNALWLV
                     KSGAHIVKFEGGGKRVITARYLVERGIPVCAHLGFTPQSVNQLGGYKIQGKTEESAEQ
                     ILNDAKAMSDAGVSFVLLEMVPAALAKKITEGISAPTIGIGAGVHCSGQVLVIQDLLG
                     IYTGASSKDPKDFKAPRFAKNFLIETNSVQQAVKNYVQEVKNETFPDVEHSY"
     gene            complement(9475..10122)
                     /locus_tag="PKL42_04540"
     CDS             complement(9475..10122)
                     /locus_tag="PKL42_04540"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNU66376.1"
                     /translation="MSIFTRFPYIVVEGPIGSGKTTLARMLADKFPVDYLSEKAEANP
                     FLPRFYQDAQRYALPTQLFFLFQRANQIKDLSQRDMFAKPIVADFFLEKDALFARLNL
                     DDEEYALYHNIYTHLQLKAPKPDLVIYLQTPIDALAERIEERNVHYEQNMPREYLARL
                     SDAYSEFFHHYDATPVLIVNNEKLNIAKNPEALDLLLNRIMQIKSQREFFNPNFE"
     gene            complement(10119..10622)
                     /gene="folK"
                     /locus_tag="PKL42_04545"
     CDS             complement(10119..10622)
                     /gene="folK"
                     /locus_tag="PKL42_04545"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNU66377.1"
                     /translation="MKHAFIALGSNLADPILQVKNAFSALSKIPKTRVIKGSSLYRTA
                     PIDCPQTTEQSVPDFINAVVEIETTLSPEALLTALHAIENAAGRERPYINAPRVLDCD
                     LLLYENIVQNTAKLTLPHPRMHTRGFVLLPLFEIAPQLIVPKHGKIAQLMTPQLAQGI
                     QKLENHL"
     gene            complement(10629..11996)
                     /gene="pcnB"
                     /locus_tag="PKL42_04550"
     CDS             complement(10629..11996)
                     /gene="pcnB"
                     /locus_tag="PKL42_04550"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HNU66378.1"
                     /translation="MIKRFLQKVFTKRTKAHLAIELADASDTSAAKKISAKVHKINKN
                     LISQAALKTCEGLQKAGFEAFIVGGAVRDLLLNFKPKDFDVATNATPEEVNRIFRRSR
                     IIGKRFRLVHVLWGNETIEVSTFRGHHLAEGDAKTADSGRILRDNIFGSLEEDAARRD
                     FTANALYYNPAKDEVLDFHNGVADVNAKLLRMIGNPTTRYQEDPVRMLRAVRLSAKLG
                     MKIAPDTQAPIAKLVDLLQDVPASRLFDEMLKLFLSGHAVESVNALRAQHLHHGLLPM
                     LDVVLEQPLGERFVMLALKNTDDRINQGKSSNPSFLFATLLWHEVLTAWQTYKNEAPP
                     IPALYMAMNEVIASQAEKLAIHNRYTATMKEIWAMQPRFEQRAGKRPFSLLTHPRYRA
                     GYDFLLLRCESGELPMELGEWWTAFANAEGEARTAMLQADTNPNKRRKRNRKKVSANK
                     SEPIS"
     gene            12117..>12697
                     /gene="murI"
                     /locus_tag="PKL42_04555"
     CDS             12117..>12697
                     /gene="murI"
                     /locus_tag="PKL42_04555"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNU66379.1"
                     /translation="MPELLPNHENPTKLTSASQPVGVMDSGVGGISVLKHIHALLPDE
                     DLIYVADSKYAPYGSKTAAEITERCFAVADFLIAQHVKALVVACNTATAAAIDAMRQK
                     YTIPIIGMEPAVKPAASASKNGVIGVLATVGTLKSAQFAALLESYGRNVKVVTQGCTG
                     LVECVERGELHNENTKALIQQYCAPLLTEGADT"
BASE COUNT         3302 a         2729 c         2975 g         3691 t
ORIGIN      
        1 tggatagttt gggtaaacaa atcgcttcac ccttgttgaa tataagtgaa gatccgcata
       61 tcaaaaaagg cttggcgagc agtccgtttg ataatgaagg cgtcgcaacc cacgcgcgac
      121 aattagtgaa agatggcgtg ttgcaaggct acgtgcttgg cagctactct gccagaaaac
      181 taggaatgca aacaacgggt aatgcaggtg gcaaccataa tttaatcgtg caatctagcg
      241 ggcaggattt tactgcgtta ttgaagcaaa tgggaacagg attgctggtg acagagttac
      301 tggggcacgg catgaatatg gtcactggtg actattcacg cggtgcggcc ggtttttggg
      361 ttgaaaatgg cgttattctt caccctgttg aagaaattac cattgccagc aatatggctg
      421 acatgctcaa aaatattgct gcgattggca atgacatttt ggtacaaggt tcaaagcaag
      481 tgggctcaat tttgattgag cgtatgacgg tcgcgagcga ataacacggt gttttaatcc
      541 agcaaaaaag cgtagtgtta ataaaaaagc ctaatcatct gattaggctt ttttatttgt
      601 tacgaaaagc agtttatttt gtctcagctg ctggttggtt ttcagtggct ggtgttgtag
      661 cattatctaa cgtgtcatct tcttcaaaga attcgtcgtt tgatagattt cgttttgcga
      721 gattgtcatt cacttggttt tcacgtcttt gcaggtaggc gtcacgcatg aatgcgtatg
      781 ggtctaatga agcttcgtcc accaaatcac tcgcgtctag cagttctgta cgcttgtcaa
      841 taaactgcgc taggcgcaat tgattgtgta ggcgaatttc accaatgtta tgtgtgtagt
      901 gtatcgggtc ggtagttgtt gtatccacta ccaagccagt ggtgtcgcgt agtgttgatg
      961 ggcctaaaaa tggtaaaacc aaataagcgc cattgcccac gccccaaaaa cctagggttt
     1021 gaccaaagtc ttctttgcga agcgcaatat tatctttgcc ggccacatca ataaaaccag
     1081 ccaaaccgaa cgtgctgtta atgacaaatc ggcctgcatc agtaaaggca tgtgcaaatt
     1141 taaactgtaa taaatcattt aaaacagtag taatcgagcc taaattggta aaaaagttat
     1201 taaatccagt gcgaatcggt gaaggggtga ctgctttata agccgtcgca accggtttaa
     1261 atacggcttt atctgtgaca tcgttaaatt tatacaccgc gcggtttaca ccttcaagcg
     1321 ggtctttatt ggcttgtgtt gcacagcctg aaagtatggt gacagcacat aggctagcga
     1381 tagcaagcgt tgttttaata taattttttt gtggatgacg catttaatac cccgatatga
     1441 ttgatttttc actaacgata tttttgtttt ttgtagatgg atttacaatt attaacggaa
     1501 aaatctttcg ttactttaac ctagcgtgat tgggttgtct agtgggtgaa cgtgaatcta
     1561 cttgaaaaat ttacaattac ctaatgtttt taaagtaaat tatgcaaaaa aaccaattat
     1621 ttgtgggctt caaatggttg ggattctgtt gcctgatgca gcacatgacc atcatcaaat
     1681 gaaatttgat aacccaatgt ggttttttga tacattaaaa ttgtagggtg catcacgcgc
     1741 ttacaagcgt tgaagccgtt tatttggctt aaatgctctg gatattgcag gttttgttgg
     1801 tagaaagtgg tcagttgctc ccgtatgacc tcaccctgcg tcacgcattc gttataggcg
     1861 cgcatatacg attttgtgcc taaataaaaa ctatagctgt agcttgatac aaaaacgcat
     1921 cctaatatga ccttacccca caaattggtt ggcgcagaaa agagcgtgaa aaagacggca
     1981 gggataatac taaaaagcaa aaagttacca aacagttttc cgtctgcctc acctgcccaa
     2041 aaggctaggc cacaaaagag tacgaatagg ccaacaaaaa tgcgccactt cacgcaacgg
     2101 cacccatttt aaataatggc aaatacatgg ccacgacaac ggttgcgata atgccaatga
     2161 aagtcagtaa gtctgcaaaa aatatgattt taaaggcatg gttcatttca gtggtgaccc
     2221 gtgattgaac cataaaaaaa ataacaatca cgctcatata gattggcatc agcagcaacc
     2281 attttcgata atcaacaacg atgcttgtga gccaaggtaa atctgcgcca aaacttgaaa
     2341 aaaccgtttc atataaggga atggcgaatt gcgcgcctat agaaaaaata agcgccactg
     2401 caaacatgat gcaataactt atcagaattg ctttgcttct gggaactagg tttttgggcg
     2461 catttttttc aggcgtttgc atggtggttg agctgttctg ctgttgttca atatgcgtca
     2521 ttatagtgct gttttaatgc gcgtgaatat gatcaaaagt acattgaatt ttctgcgagg
     2581 aaaattttat gccctgtgag cctttattta taaggctcgc agggcagtca aaaaggttgt
     2641 ggtgtaaaaa tgggtgtttt tggcttattt tgttggcgta tttttataca acgccaaagc
     2701 tttttcacgc tggctggcat ggtcaacaat tggatttggg tagtctttgc ctaggttcag
     2761 ttggagcgat tgttgccgta gtggtggaat cagccaaggc gcatggattt ctttatcatc
     2821 acaagcgcct aactcaggca catatttgcg aataaatttg cctaggctgt caaaacgttc
     2881 gctttgtgtg attgggttaa aaatgcgaaa ccaaggctgt gcatcgcagc ctgtgctggc
     2941 cgcccattgc cagccgccgt tgttggctga aaagtcaaag tcaatcagtt tttcggaaaa
     3001 atattgctcg ccccagcgcc agtcaatcag taaatctttt accagaaagc tggccgctac
     3061 catgcgtaag cggttatgca taaagccggt ttggttgagc tggcgcatgg cggcatctac
     3121 cagcgggtaa cccgtgttgc cctcgcacca tgctttgaaa tgttctgcat ggttggggaa
     3181 gtttaaggat tcaaattcgg ccttataagc gcggccgctg gccacctgcg gatgatggtg
     3241 cagaatttga aaataaaaat cgcgccaaat caattcattg agccatgttt cagcaccggc
     3301 accgccgcgc tgccaagcct cgcgcgctag gtggcgaata gatacggtgc caaagcgcaa
     3361 atgtaccgat aaatacgaaa cgccctttac cgcaggaaaa tctcgcgctt ctttgtagca
     3421 ttgcatgcgc tcagtaaaat cggcaaataa ttgtgcgccg ccgtgcatac cagtgggcag
     3481 gcgcatggcg cttaaattgg tgcgcgcaaa accaaggttt tctaaagata tgagcggcgt
     3541 tggctcaact tttaccaagt gctgtatata ggcatcgact gggtaagggc tcaggtaaaa
     3601 atcattcacc tttttgagcc aagcgttttt gtagggcgtg aatacactat aaggtttgcc
     3661 cacttggctc aagacttcct cttgctcaaa aatgacttgg tctttgtaat gatgcaaggc
     3721 aatttgatgt ggctgaagcc gttgggtcac caataaatca cgcgcaatgg cggcgggttc
     3781 atagtcatga ttggtaaaaa ctgcctgcac gcccagttgc aaggctaggg ctggaacttc
     3841 ctcacgcgca ttgccgtgca acaccattaa atcgccgcct ttggcttgca gcgcggtttt
     3901 gagcactttg acgctttccc aaataaattc tacgcggcgg tcggctttat ctttaagcgc
     3961 attgagaatc tccgtatcaa acacaaagac acaatatact tgggtggaat ttttgagggc
     4021 atgataaagc gctgcatggt cgtagtcgcg caagtcgcgg cgaaaccaga ccagagattt
     4081 ttcgtaatgc atattactct cggtggtagg gatggccttg attgaccgag tgcgcgcggt
     4141 aaagttgctc gcacaacaat acgcgcacaa aggcatgcgg caaggtgagt ttagataaac
     4201 cccaaagcca atcggcttgt tgttttaggc tagcgtgtaa gccatctgca ccgccaatca
     4261 caatgctgac atcgcgccct aggccttgcc aaaattgcat tttttcagcc agtaacaagg
     4321 tggtgacttc ttggccgcgc tcatcgcagg cgattaaaaa atctttgcct gcggcctcta
     4381 aaatgcgctt cgcttcggcc tcttgcacca ccacactatt tttgccatcg gcacgtttgt
     4441 ctggtttgat ttcgataatt tcaatagcga gttcgcgcgg catgcgcttg atatactctg
     4501 cgcaagcggt ttcaacccaa gcgggcattt tatggccaac ggagataatg cgcagtttca
     4561 ctttaagctt ttgattgcgc taattgaatc actgcatcaa ttgcagcgcg ggcttgcggg
     4621 ctgtttttcc agcaagtagc gcgcgtaagg tttgcgccat attctaaaat atcttgtgtt
     4681 ttagcttgcg cgagttgatt aagtgacgca aagcccattt cttctaaacg cgagataacc
     4741 gtattaccta cgccttttag cttgagtaat gcggcgcgtt cttcaactga aaaaggcata
     4801 taacctcaat tagctggctt taatatgccc gcggcggctt tctgcctcgc cccataattg
     4861 ctcaaggttg tagtaagcac gggtggttgg caccataatg tgtacaacaa tgtcatctaa
     4921 atctaccaac acccattcgc cttctttttc gccttctgta ccgcgaatat gaaagccttt
     4981 ttctttgagt ttttcacgta cattatcagc aatggctttg cactggcggg tagagttggc
     5041 agaggccacg atcatgtaat tggtcatgct ggtgagtttg cgcacgtcca tcacgctgat
     5101 gtcgaaacct ttaatatctt caatcgcgtc aaccacggcg gttttcatgg cttcaaggtc
     5161 aaaaggggcg gaagttgggg tgttgctgtt ggttgtcatt tatgcggcct gtgctggaat
     5221 tgcgttacgt tgtgtttgta tgcggttttt ttcatccaca tacacgagtt ggggtttaaa
     5281 tttttcaagt tctaattcat ggtagtcggc atagctggca ataattaaaa tatcgccagg
     5341 atttgcttta tgcgcggcag cgccattcac tgaaataatg ccagaattgc gctcaccacg
     5401 gattgcgtag gtgctaaaac gctcaccatt ggtgacattg taaatatgaa tctgttcata
     5461 ttcacgaata ttggccgctt ctaataacac ctcgtcgatc gcacacgaac cttcataatg
     5521 cagctcggca tgtgtggtgt gaacgcggtg taacttagat ttcaacatgg ttctttgcat
     5581 gtgatttttt ggctttttaa tttaaagcgc tattataact ttatatcgct acaaacttca
     5641 cgctcacttc acataagcac tttaatattg ctagatgaaa aattgtcata cggtgaaaac
     5701 attaaagcgg ttaaaacttt aaagaggaat gcaatgacag acttagcccc acaaaacaga
     5761 gagcatcggt ttattttaag catggcttta ttgcaaggcg ttgcgctgtt actcatttgg
     5821 ctttgggtcg atacattaag caatgcacga caatatttta atgtgatttt gccgctgtac
     5881 gtgctggcca ttagttttcc gcttagctta atgttgcttg cgcatcagcc acgcaaattg
     5941 gccttgagtt tggcgggcgc gtttagcctg attgcggcat tttgcgccat gtattttggt
     6001 gatgtgagct atgtaaaagg tctgccgcgt tatggcgtag agggcttggt gtttcaattt
     6061 ggtttttgca tggtgatggc atggtttatt gccgtgacct ttattgagca tttttgccaa
     6121 tatggtcgtt ggtctaacca ttatgaatct ttgtatgatt attcttggcg caacgcggta
     6181 aagcttatta cttcgggtat ttttactggc ttattttggg cagctttatt gctattggca
     6241 ggcttgttta aagtactagg catcaccttt tttaaaacca ttattgaaga tgtttacttt
     6301 atttaccccg ccacagcggt ggcgtttggc ttaggcattt cactctataa cgctaagcaa
     6361 gatgcgcttt ctgagtttaa acgtgcgatt ttgcaagtgc tgggctggtt attgccgctc
     6421 gtgagtttga ttttgctggc gtttatgatt actttgccat ttaaaggctt agcttcgtta
     6481 tggggcacgg gctacgccac cagcttaatg ttgagtttgc tggcgctcat ggtatttttg
     6541 cttaacaccg cttttcaaga tggtaaacaa acacaatacc ccgcttggtt gttaaagcta
     6601 agcaatttag ccgtgttgac catgccaatt tacgccatgt tgtgcatcta tgcgctgtat
     6661 ttacgcgtca atcaatatgg ctggacggac gaccgcgtat gggcggcaag cattaccttg
     6721 attatggcaa tttatagcgt gggttatgcg tttgccgcgt ttcaatcatt taaccaaaat
     6781 acttggatgc aggcgattaa atcagtcaat gtaatcgcgg ccgtttgttt aatggcgttt
     6841 atcggcttgc tctattcgcc catactcagc cccatgcgca ttggtgtgca aagccagctc
     6901 gcgcggttgc acaatgaaac aatagcagca agccagtttg attatgaata tttacgtttt
     6961 agtggcggaa aatatggcga agcggctctc aaaaacttgc tggctgatac cacccacccg
     7021 caagcggcca cgattaagcc attggtggaa gctacgctca aagcgcaata tcgtcattac
     7081 cgctcagtcg aaacaaagaa gccgccagtg accttgcaaa cattgcaagc gcatttcaat
     7141 atttacccta aaggcacacc tattgaagcg gcattttttg aacgtgtgtt gcaagagtat
     7201 caaaaccatg aagtatattt tgcatgttta gattttccac aagacaccga ttgcgcaaac
     7261 accaaaccaa aagaccaatt gctggcagtg gacttaaacc aagacggaca cgttgagatt
     7321 gttttttttc agcgcaatgg tggtgggcgt gcctataaaa aagaagccaa cagttggata
     7381 atgtttgcgc acttaaacaa ctattgctgt gaagcacaag aaacaacaga aacatttcaa
     7441 aaaaatctgc aaagccagtt agaagcagga gaggtaacgg ccacagcgcc acaatggcaa
     7501 gatttaaaag ttggtaataa aaaataccag ctcaatgttt accgttaatt aggagtggtc
     7561 tgtggctgtg aagaatgtga ttcgcggtta taaatgcact tcaatattgt ctatcaagcg
     7621 ggttttgcct gcttgattat tgctgatttt agctgcgccc agcaccacta aatgctggtc
     7681 ttgtgcggct gcgggctgca aggtaagggc agaacgaata gaaatgtaat caacttccca
     7741 accttgttga ttaagttgtt gcgtggccgt attttctaag gcactaaaat ttttatcacc
     7801 atttttaagc gcttgcacca tgctttgcaa tgctgcattg agttgcgcag cttgcgtgcg
     7861 ttgcgcttca tttaaatagc cattgcgtga gctcatcgcc aagccactgg cttcgcgcat
     7921 ggtatcacct gcaataattt caataggcaa attaaactgc ttcaccattt ctttgatgat
     7981 aaaaagttgc tgaaaatctt tttggccaaa cacggcaacg tctggctgta ccatattaaa
     8041 aagcttcatt accaccgttg ccacgccaga aaagtggcct ggtcgagacg cgccacaaag
     8101 ctcactagca attggcggtg gggtgatggt catggtttga ttgaggtttt ttccgtcaaa
     8161 gtctgggtag atttctgtca cattaggcgc aaacacaata tcagctccag ccgcttttag
     8221 tttttcgcaa tctgcctcga gtgtgcgtgg ataactgcca aaatcttcat ttgcgccaaa
     8281 ttgcagcggg ttcacaaaaa tgcttacaac aacgcagtgc gcatgttgtt tggcaatttc
     8341 aaccagttga agatgccctg catgcagatt gcccatagtt gggacaaaac tgataccaga
     8401 ttggtttttg agtgtggcgc gaagttcggc gatggtgtgg acaatttgca tgcgcgcatt
     8461 ttagcattgc ctatctgctt acagaagaat tttacttttt gtggtaaaac aatccatcaa
     8521 tttgcattaa tatattgctt aatttttaag caaaatatcc tcgagaaaaa aattatgctc
     8581 tggaagcctt tatttacaag gcttgcagag catcaaaaaa ggttgtgtat gatttaggtg
     8641 ttttttaatt gattttatgc gcagtttaat aactatgttc tacatcagga aaagtttcat
     8701 ttttaacttc ttgcacgtaa tttttaaccg cctgttgcac gctattggtt tcaatcaaaa
     8761 agtttttagc aaatcttggg gctttaaagt ctttagggtc tttgctggag gcacctgtgt
     8821 aaatgcccag taagtcctga atcaccaata cttgtccgct acagtgcaca cctgcgccaa
     8881 tgccgatggt gggtgcgcta atgccttcgg tgattttttt ggcaagcgcg gcgggtacca
     8941 tttcgagcaa cacaaaactc acgcctgcgt cactcatggc cttggcgtca tttaaaatct
     9001 gttcggctga ttcttcagtt tttccctgaa ttttatagcc acctaactga ttgaccgact
     9061 gcggcgtaaa gcctaaatgc gcgcaaacag gaatgccgcg ctcaaccaaa taacgtgcgg
     9121 ttataacgcg ttttccgccg ccttcaaact tcacaatgtg cgcgccactt tttactaacc
     9181 aaagcgcatt tttataagcg gcttcgttat cctgctcata actgccgata ggcatgtcgg
     9241 ccataatcac cgtgtttggc gcaccgtttt taaccatctt agtgtgataa gtcatgtggt
     9301 gcatgctgac attgagcgtg ttctcagcac cttgcaacac catgcctaaa gagtcgccca
     9361 ccagcaacat gtccacgcct gctaattcca ttaatttggc aaaggtggtg tcatagcagg
     9421 tcagcatggc aattttttcg ccagcggcta ctttggtttg aagtttagtt aaactcattc
     9481 gaaattcggg ttaaaaaatt cgcgctgact tttaatctgc atgatgcggt taagcagtaa
     9541 atccaatgct tcaggatttt tagcgatatt taatttttca ttgttcacaa tcaacactgg
     9601 cgtggcgtcg tagtggtgaa aaaattcgct gtaggcatct gataaacgcg ccaaatattc
     9661 gcgcggcata ttttgttcat aatgtacgtt gcgttcttca atgcgctcag ccagcgcatc
     9721 aatgggcgtt tgcagataaa tcaccaaatc aggcttgggc gcttttaatt gtaagtgcgt
     9781 gtaaatgttg tgataaagcg catattcttc atcatctaaa ttgagccgcg caaacaatgc
     9841 atctttttct aaaaagaagt cggccacaat cggcttggca aacatatcgc gctggcttaa
     9901 atctttgatt tggttggcgc gctgaaacaa aaagaatagc tgcgttggta gcgcgtaacg
     9961 ctgcgcatct tgataaaagc gcggcaaaaa tgggttggct tcggcttttt cagataaata
    10021 atccacagga aatttatctg ccagcatacg cgccagcgtg gttttgccgc tgccaatcgg
    10081 gccttccaca acaatataag ggaatctggt aaaaatgctc ataagtgatt ttctagtttt
    10141 tgaatacctt gggcgagttg cggcgtcatc aattgcgcta ttttgccatg ttttggcaca
    10201 attagttgcg gtgcgatttc aaatagtggc aataaaacaa atccgcgggt gtgcatgcgc
    10261 gggtgcggta gcgtgagttt ggcggtattt tgcacgatat tttcataaag caataagtcg
    10321 caatccagca cgcgcggcgc gttgatgtaa ggccgctcgc gccccgcggc gttttctatg
    10381 gcgtgtagtg ctgttaataa ggcttctggc gaaagcgtgg tttcaatttc aaccacggcg
    10441 ttgataaagt ccggcacgga ttgttccgtg gtttgcgggc aatcaatcgg cgcagttcta
    10501 taaagcgatg agcccttgat gacgcgtgtt ttaggaattt tactcagcgc agaaaacgca
    10561 tttttaactt gtaaaatggg gtcagctaaa ttgctgccta atgcgataaa agcgtgtttc
    10621 atgcgttttt aactaatggg ctcacttttg ttggcgctca cttttttacg gtttcttttt
    10681 ctgcgcttgt tagggttggt gtcggcttgc agcattgccg tgcgtgcttc gccttccgca
    10741 ttggcaaaag cggtccacca ttcaccaagt tccatcggta attcacctga ttcacaacgc
    10801 agcagtaaaa agtcatagcc tgcgcggtag cgcgggtgcg tgagtaaact aaacggccgc
    10861 ttgccagcgc gttgctcaaa gcgcggttgc atagcccaaa tttctttcat ggtggcggtg
    10921 tagcggttat gaatcgccaa tttttcagct tggctggcaa tcacttcatt cattgccata
    10981 taaagcgcag gaatcggcgg ggcttcattt ttataagttt gccatgcggt cagcacctca
    11041 tgccaaagta gcgtggcgaa taaaaagctg gggtttgaag atttgccctg attaatgcga
    11101 tcgtcggtat tttttaacgc cagcatcacg aagcgctcgc ccagcggttg ctccagcact
    11161 acatcgagca tcggcaataa gccatgatgc agatgctgtg cacgtaaggc attgacgctt
    11221 tcaaccgcgt ggccggataa aaacagtttc agcatttcat caaacaggcg gctggcaggc
    11281 acgtcttgca gtaaatctac cagtttggcg atgggcgctt gtgtgtcagg ggcgattttc
    11341 atgcctaatt tggctgacaa gcgcaccgca cgcaacatgc gcacagggtc ttcttgatag
    11401 cgcgtggtcg ggttgccaat catgcgtaac aatttggcgt ttacatcggc tacgccattg
    11461 tgaaaatcca gcacttcatc tttcgcaggg ttgtaataga gggcgttggc ggtaaagtcg
    11521 cgccgtgctg cgtcttcttc taaactgcca aagatgttgt cgcgcagaat gcgaccgctg
    11581 tcagcggttt tggcatcacc ttccgctaaa tgatggccgc gaaaagttga gacttcaatc
    11641 gtctcattgc cccacaatac gtgcaccagc ctaaaacgtt tgccgataat gcgagagcgc
    11701 cgaaaaatgc ggtttacttc ttcaggtgtg gcattggtgg cgacatcaaa atcttttggc
    11761 ttaaaattca atagcaaatc acgtacagca ccaccgacaa taaatgcctc aaagcccgct
    11821 ttttgtaagc cttcgcaagt ttttagtgca gcttggctaa tcagattttt gttgatttta
    11881 tgtactttgg ccgatatttt ttttgcggcg ctggtatcgc tcgcatccgc aagttctatt
    11941 gccaaatgtg ctttagtgcg cttggtaaat actttttgta aaaatctttt aatcatcgag
    12001 gtttaatcat gccaaatctt ttaggtgttc attgttgggt gcaagtgttg cacaacttat
    12061 aagccattca aaatcctata taattcaagc attataaatg atgatgctaa aacttgatgc
    12121 ctgagttact accaaatcac gaaaatccaa ccaaattaac atcagcaagc cagcctgttg
    12181 gcgtcatgga ttcaggtgtg ggtggaattt ctgtattaaa gcacattcac gccttactgc
    12241 cagatgaaga tttaatttat gtggctgaca gcaaatatgc gccttacggc agcaaaactg
    12301 ctgctgaaat caccgaacga tgctttgccg tggccgattt tttaattgct caacatgtta
    12361 aggccctagt cgttgcctgt aacactgcca cagccgccgc catagatgcg atgcgtcaaa
    12421 aatataccat tcccattatt ggcatggagc cagccgttaa acctgccgct agcgcgagca
    12481 aaaatggcgt tatcggcgtg ctggccaccg ttggcacact taaaagtgcg caatttgccg
    12541 cgttacttga aagttatggc cgcaatgtaa aagttgtgac gcaaggctgc acaggcttgg
    12601 tggaatgcgt ggagcgcggc gaactacata acgaaaatac caaagcactg attcaacaat
    12661 attgcgcgcc attactgact gaaggggctg acaccat
//
LOCUS       DAOQSI010000046        12572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_4126_length_12572_cov_5.716386, whole genome shotgun sequence.
ACCESSION   DAOQSI010000046 DAOQSI010000000
VERSION     DAOQSI010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 12572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12572
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4126_length_12572_cov_5.716386"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PKL42_04560"
     CDS             <1..306
                     /locus_tag="PKL42_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination factor protein RarA"
                     /protein_id="HNU66380.1"
                     /translation="NAAYNAYNQAIAFVGQDKSRPVPLHLKNAPTKLMKALDYGKEYR
                     YAHHEPEAYAAGVHYFPDELHDKNAVPQFYMPTPRGLEGKITEKLAHLRELDNKAKK"
     gene            313..1674
                     /gene="serS"
                     /locus_tag="PKL42_04565"
     CDS             313..1674
                     /gene="serS"
                     /locus_tag="PKL42_04565"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNU66381.1"
                     /translation="MLDIQLLRSDLDCVVARLSARGFDFPKAKFSELEAERKILQTST
                     EALQAKRNAASKQIGFAKSKGEDVSAIMAEVAGLGRQLVADALVLSKLQTKLQDLLLD
                     VPNLPHESVPQGKSEVDNVEVRKVGTPRSFDFEIKDHTDVGAPLGLDFDTGAKLSGAR
                     FTFMRGQVAKLHRALAQFMLDTQTEQHGYEECYTPYIVNEQTLLGTGQLPKFEQDLFQ
                     VKFDKSMTRKELMMLNPECKIDVPPDWPFQVFESSLYLIPTSEVTLTNTVRDEIVPLE
                     SLPIKLTAHTPCFRSEAGSYGRDTKGMIRQHQFDKVEMVQIVHPEKSYEALEEMVGHA
                     ENILKALELPYRVVSLCTGDMGFGAAKTYDLEVWLPAQNTYREISSVSNCEAFQARRL
                     QARFRNEQGKPELLHTLNGSGLAVGRTLVAVLENYQNADGSVTVPQVLRPYMNNLDKI
                     KPI"
     gene            complement(1793..3220)
                     /gene="mltF"
                     /locus_tag="PKL42_04570"
     CDS             complement(1793..3220)
                     /gene="mltF"
                     /locus_tag="PKL42_04570"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HNU66382.1"
                     /translation="MHKLFIVLLVLLAAACTRTDERTTSTAHSETDDANTLIFVTHNG
                     PTTYYYNGDNELAGLEYDLATLFVKDFAPEYQLKFLVVNSVSDVIPSLLKGRAHIAAA
                     NLSITRLRQELVQFSTPYQQTQQQLVTLNEENTKQNRPIQVKDFNVQLANKKLAVPLG
                     TSFEERLIQLKKSQPALQWQSIPNTNSELLLSDVANGTLDYTMADSHLVSIMQNYYPN
                     LAVAAKVGEADNLAWAFSKQANPALVKKVNAFFAKIKKDGTLKHLIDRYYGHSDRLNT
                     QDVTVFLARSQSILPNYAALFKQAQEQTGLDWRLIAAVSYRESHWDPLNTSPTGVRGM
                     MMLTENTAKLMGVTDRLDPVQSIPAGAKYLVKMIEKIPETVPEPDRTYLALAAYNIGY
                     AHLLDARTLAKRLKLNPDRWSDVKKTLLMLRQPDYYTTLKYGYANGSAPVIFVESVRS
                     YQNILEYHQPSHPANEQINVANLSK"
     gene            complement(3265..3942)
                     /gene="rpiA"
                     /locus_tag="PKL42_04575"
     CDS             complement(3265..3942)
                     /gene="rpiA"
                     /locus_tag="PKL42_04575"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HNU66383.1"
                     /translation="MNDKQLVALEAAKFVKNGMLVGLGTGSTANYFIDELARLQRENG
                     LSITTVASSTISAQRAQSLGLPIVALEHISQIDLYVDGADEISPDMTLLKGRGSDLVK
                     EKLLAKAATQFIVVADESKLVSRIGQNFVIPIEVIPFAWQLTKKSLEDAGGIGHLRPN
                     AAKDGFWITSHGSYVLDLTFDADFSANALNELINNTPGVVEHGIFYGLTDAVLIAANG
                     KVEARWA"
     gene            complement(4119..4679)
                     /gene="efp"
                     /locus_tag="PKL42_04580"
     CDS             complement(4119..4679)
                     /gene="efp"
                     /locus_tag="PKL42_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNU66384.1"
                     /translation="MKTAQELRAGNVIMVGDVPLVVVKAEYNKSGRSTAVVKMKFKNL
                     LTEAPSEAIYGAGDKFDVIVLEKKEATYSYFADPTYVFMDADYNQYDVDAEFMEDVLP
                     YLEDGMPADIRFYNDKAISVELPTTVVREITYTEPAVKGDTSGKVMKPAKIATGYEIS
                     VPLFCAQGDKIEIDTRTGEYRNRVMK"
     gene            complement(4737..5840)
                     /gene="earP"
                     /locus_tag="PKL42_04585"
     CDS             complement(4737..5840)
                     /gene="earP"
                     /locus_tag="PKL42_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HNU66385.1"
                     /translation="MQNGTDQRWDIFCRIVDNFGDIGVCWRLSQQLANTHQLPIRLFI
                     DDLETAKKIIPGYQPELGTQIINHVEIWAWPNDDDAIQPAEVVIETFACGIPQRYLSA
                     MQPQTKWVNLEYLSAEKWIDEFHALPSPQASGLSRHFFFPGFTEATGGLIREPNIVSY
                     ADTHRTNLAEQTLKISLFAYPNAPIEDLLHILQASQQSTVVYVPNSSILPQVESFLGI
                     TQSNPHETYLRDKLHIKMLPFLSQDNYDALLATCDINFVRGEDSWVRGIWAAKPLIWQ
                     PYFQTENTHMVKLNAFLKLFYTACHEETFQAIAELNGGWAQGSISTEIWLNYVRQLPA
                     IRAITQASTMALAAQPDLASKLVIYLQKISVQV"
     gene            complement(5851..6363)
                     /gene="greB"
                     /locus_tag="PKL42_04590"
     CDS             complement(5851..6363)
                     /gene="greB"
                     /locus_tag="PKL42_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HNU66386.1"
                     /translation="MAKAKLAVSDEKNYITPEGHAALEAEFQQLLKVERPEVVRVVSW
                     AAGNGDRSENGDYIYGKRRLRQIDSRLRFLMQRMDLAIIVDPATQVGLEKIFFGAWVI
                     LFSLKNDTEHSYRIVGKDELEPSKGYISWVSPLAKAMLGKSVGDRIKVSTPAGDEEYE
                     VIDIQYNLPK"
     gene            complement(6366..6800)
                     /locus_tag="PKL42_04595"
     CDS             complement(6366..6800)
                     /locus_tag="PKL42_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66387.1"
                     /translation="MLAKLLQLLKYSIWLLLVPIGLFALYTWATLTWVYSSGERAGYV
                     QKLSEKGWVCKTFEGELILVSMPGTQAEKFFFTVRDKAVAKKINETVGKRVRLVYEEH
                     IGIPTTCFGETGYFVHDVQPIDQVWPEQQLLDTQPMNPQTAQ"
     gene            6960..9659
                     /locus_tag="PKL42_04600"
     CDS             6960..9659
                     /locus_tag="PKL42_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNU66388.1"
                     /translation="MSKQTLILCPSARLARSIQNDIALQNSQSGQVVWHSPAVMTLSQ
                     WLENVINTAFLSGQFTQVQPYALSTLNEQLLWQEVITQSLKKNAFGALFDVAGLAQAA
                     MEANRYQVAWHLHLPREHLAEESRQFLHWQRAFAARCAELNVLEQVRYVDWQLAQLAA
                     GFVMLPSHIAFAGFDQTAPQELRLRKILVCQGVEVSEYATTADTVANAQHVRLENQAA
                     ELRAAVAWAKDLLAHQPKAKLAIVVPQLNTVRNQLADLLDDVFYPESVRPGLFEMPRN
                     YNFSLGTPLAQQPIIQAALNLLRLLSHSSANQPMPQSDYSAVLLSPFWSASTTEADAR
                     AMLDATMRDKLPMQFKLTNLLALAEKQLKNGLAITKFLADLKAAIALIPNKKVPPSQW
                     AQTITTLLTALHWPGERAVTSMEYQAQNAWQKALQQLAQMDVLQKNIGLAEAVSLLQQ
                     ICTAMIFQAETEYAPTIQILGVMEALSAPVDALWCMHMNDHIWPPPARPNPLLPAYLQ
                     RQAMLPNADNTVQAAFAETIHQRLLHSAPQIIFSSSMSERDSQLRPSPLMKNMAHAAH
                     EMPLANTLAEQLSAEGNTDLTFLDDHIAPAIQADEHVRGGTGLFKAQAVCPAWAFYQY
                     RLGAKALKTPTDGIDSMERGSLVHGVLEQFWRKRHFADLRDMSVDALYLAVNHAITQT
                     LQVFAAENSVSANVLALEHERLFKLITDWLLYEKLRGVGFNMVACEAEKKVSICGIEV
                     TLKIDRIHALENGGLEFVDYKTGQAPKITSWAETRITEPQLPIYAAFYMEDAEHVTGV
                     QFGMVKTAEHMFTGVSAENFDTEPEKRKPVFIRAFSDWASLLTHWKSSIEAIAQEIKS
                     GEAAVRFNDEQALAYCEVLPLLRLPERKLQFERFANNADEGAV"
     gene            9833..>12572
                     /locus_tag="PKL42_04605"
     CDS             9833..>12572
                     /locus_tag="PKL42_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNU66389.1"
                     /translation="MTAQCELNLATDEKNRQRALDLTSFIVEAPAGAGKTELLTQRYL
                     KLLQTVSAPEEIIAITFTNKAAAEMRVRILDSLIKASHRDKPAAAHKQITYALSLKAL
                     EIAEAKHWQLIENPARLRIFTIDSLCAHLARQMPLMSRFGAQPRVAEDAGALYTQAAE
                     HTLALLEAPAHTEVVKYALRYVDNNAEQLKGLLVKMLEKRDQWLHHAQREVDVGVLQQ
                     TLRHLVEQELANIAQVFNARLQFALMPLARFAASQLPCEHAIALLRDWDTALMTHAEA
                     LPMWQSVRELLLTNDDSIRASISKNIGFPPEFKDEKAQFKCFLEALQAVPNSAESLAK
                     VKKLPNIANTQDAWQIISTLSQLLNLAVAELWLVFQRAGEVDFVEIATRATQALSHDF
                     GEPTDLALKLDYQIQHLLVDEFQDTSPSQIELLKLLTLGWQVGDHRTLFCVGDPMQSI
                     YRFRKANVGLFIKASTQGIGGINLERLQLYQNNRSSPAVVDWINQTFEHVFPAEDDMM
                     QSAVHYRPFIATKENLTNAGVQVHPIIKRADESNALAQQREAEAVINIILQAQAANAQ
                     QKIAVLVRSKKHLAQLVRLLRRHHPDIAFQAVEIEALEGRQIVQDLLSLTHALHHRAD
                     RVHWLSLLRAPWCGLTLHDLHALAGQNLHATIWSLMQNAAVVQRLSADGQKRLIHVRE
                     ILAEAFATQGRMNTSRWLRGVWLMLNGSACLWAQNDVIDVQAFFACLEALDRSNQLSP
                     ERMANEITKLFASPEAQGECLQMMTIHKSKGLEFDTVILPGLGATTGGGNGDKPLLLW
                     EEVPIDDDTQLLAAPYLPKNQRNHDSVSPYDYLEMLESTREKNEAARVLYVAATRAER
                     CLHLVGIANQKADNSIAPTKNTYLDLLWEPLAAHYEMPVLQPDAAANSEDDIAFFTP"
BASE COUNT         3323 a         2775 c         3073 g         3401 t
ORIGIN      
        1 aacgcagctt ataacgcgta taatcaggcc atagcctttg ttggccaaga taaatcgcgc
       61 cccgtgccat tacatttaaa aaacgcacct accaagttga tgaaggcgtt agattacggc
      121 aaagaatacc gttatgcgca tcacgagcca gaggcttatg cggcaggtgt gcattatttt
      181 cccgatgaac tacatgataa aaatgccgta ccgcaatttt atatgcccac accacgcggg
      241 cttgagggta aaattacgga aaaattagcg catctgcgtg aactagacaa caaagccaaa
      301 aagtagagag aaatgttaga tattcaacta ttgagaagtg atttagattg cgttgtagca
      361 cgtttgagtg cgcgtgggtt tgattttccc aaagccaagt ttagcgagtt agaagcagaa
      421 cgcaaaatac tacaaacaag tacagaggcg ctgcaagcta agcggaatgc ggcttctaag
      481 cagattggct ttgcaaaatc taaaggtgaa gatgtttctg caatcatggc ggaagttgct
      541 ggtttaggta gacagttggt agctgatgcc ttggtgttaa gtaaactgca aactaagcta
      601 caagatttat tgttagatgt tcccaatttg ccgcacgaga gtgtaccgca aggcaagtca
      661 gaggttgata acgttgaagt acgtaaagtg ggtacaccgc gcagttttga ttttgaaata
      721 aaagaccata cggatgtagg cgcgccgctg ggtttggatt ttgatacagg cgcaaaactc
      781 tctggtgccc gctttacctt catgcgcggg caggttgcta agctgcaccg tgcattggcg
      841 caatttatgt tggatacgca aactgaacag catggctatg aagagtgtta tacgccatat
      901 attgtgaatg agcagacatt gcttggtact ggacagcttc cgaagtttga gcaagattta
      961 tttcaagtta agtttgataa aagcatgact agaaaagagt taatgatgct caatcctgaa
     1021 tgcaaaatag atgttcctcc agattggcca tttcaagtgt ttgaaagtag cctttatcta
     1081 atcccaactt cagaagtcac cctcaccaac accgtgcgcg atgaaattgt accgttagaa
     1141 agcttgccaa ttaagcttac cgcacacacg ccttgcttcc ggtcagaggc tggttcttat
     1201 gggcgtgata ccaaaggcat gatccgccag caccagtttg ataaagtcga gatggtgcaa
     1261 attgtgcatc ctgaaaaaag ctatgaagcg ttagaagaaa tggtgggcca cgctgagaat
     1321 attttaaaag cgctagaatt accatatcgc gtggtttcac tttgcaccgg cgacatgggt
     1381 tttggcgcag ccaaaaccta tgatttagaa gtttggttgc ccgcacaaaa tacttaccgt
     1441 gaaatttctt ctgtgagcaa ttgtgaggcg tttcaagcgc gccgtttgca ggcacgattt
     1501 agaaacgagc aaggcaagcc tgagttgcta catacgctca atggttcagg cttggcggtt
     1561 gggcgcacgt tagtggcggt gttagagaat tatcagaacg ccgatggcag tgttactgtg
     1621 ccgcaagtgc tgcgtccgta catgaataat ctcgataaaa ttaagccaat ttaagcttta
     1681 attttacaac aacctttttc gattccctga aagccttata aataaaggct tccagggcat
     1741 tatttttttc tcggcggttt taaagcattt ttaaggcaat gttgagcgct tattatttgc
     1801 tcagattggc gacgttgatt tgttcattgg cagggtggct gggctgatga tattccaaaa
     1861 tgttttggta gctgcgcacg gattctacaa aaataacggg cgcactgcca ttggcatagc
     1921 catactttag cgtggtgtaa taatctggct ggcgtaacat gagcaaggtt tttttaacat
     1981 cagaccaacg gtccgggttg agttttaagc gctttgcgag ggttctcgca tctagcaggt
     2041 gggcgtaacc gatgttgtag gctgccaatg ccaaataggt gcggtcgggt tctggaactg
     2101 tttcaggtat tttttcaatc attttgacga gatattttgc gccagcagga atgctttgta
     2161 cggggtccag cctatcagtg acccccatga gcttggcggt gttttcggtg agcatcatca
     2221 tgcctcgcac gcctgtgggt gacgtgttta atgggtccca gtgcgattcg cgataactca
     2281 ccgcagcaat taagcgccaa tcaaggccgg tttgttcctg cgcttgttta aatagcgccg
     2341 catagttggg caaaatactt tggctacgcg caagaaacac ggtgacgtct tgcgtattca
     2401 ggcgatctga gtggccataa tagcggtcta tcaggtgctt taaagtgccg tcttttttaa
     2461 tttttgcaaa aaaggcattc acttttttaa caagcgctgg gttcgcctgt tttgaaaatg
     2521 cccaagcgag gttgtcagct tctcccactt ttgccgccac ggccaagttg gggtaataat
     2581 tttgcataat agacaccaag tggctgtctg ccatggtgta gtccagcgtg ccattggcca
     2641 catcgcttaa aagtagctca gagttggtat tgggtatgga ttgccattgc agtgctggct
     2701 ggcttttttt gagctgaatc agccgttctt caaaacttgt gcccagtggt acagccaatt
     2761 ttttgttggc taactgcacg ttaaagtctt ttacttgaat agggcgattt tgtttggtat
     2821 tttcttcatt taaggtgaca agctgttgct gtgtttgttg gtaaggcgtg gaaaattgaa
     2881 ccagttcttg ccgcaaacgg gtaatagata aattggctgc agcaatatgc gcacggcctt
     2941 ttaatagcga ggggatgaca tcgctcacac tattgaccac taaaaattta agttggtatt
     3001 caggcgcaaa atccttaaca aataaagttg ctaaatcgta ttcaaggcca gccagctcgt
     3061 tatcgccatt gtaatagtaa gtcgtagggc cgttatgtgt gacaaaaatc agggtgttgg
     3121 catcatcggt ttcagaatgt gctgtagaag ttgtgcgctc gtcagtgcgc gtacaggcag
     3181 ccgccaacag cacgagcagc acaataaaga gcttatgcat gtaaccctga tttagcacat
     3241 tcactacaag aaaacgcgcg tgcattaagc ccagcgcgcc tctaccttgc cattggcggc
     3301 aatcagcacg gcatctgtta ggccataaaa aatgccgtgc tcaaccacgc caggcgtatt
     3361 gttgatgagt tcattcagtg cgttggcgct aaaatcggca tcaaaggtta aatcgagcac
     3421 ataactgcca tgcgaggtaa tccaaaagcc atcttttgca gcattagggc gcaaatggcc
     3481 aatgccaccc gcatcttcaa gacttttttt ggtgagttgc catgcaaatg gaatgacttc
     3541 aatcgggata acaaaattct gaccaatgcg gctgaccaat ttgctttcat cagccaccac
     3601 aataaattgt gtggccgctt tggctaacaa cttctctttg actaaatcgc tgccacggcc
     3661 tttaagtaag gtcatgtcgg gtgaaatttc atctgcacca tctacataga gatcaatttg
     3721 gctaatatgc tctaaggcga caataggcaa accaagtgat tgcgcacgtt gggcgctgat
     3781 ggtggagctt gcaacggtgg tgatgctaag accattttca cgttgcaggc gtgctagctc
     3841 gtcaataaaa taattggcag tagagcctgt acccaagccc accaacatgc catttttaac
     3901 aaatttagcg gcttctagcg cgactaattg cttatcgttc atactgcctc cagcaaagtt
     3961 tttattgcga tggataagta tagtagcgcg aattttaatg gcttgtatat aaaaaccttg
     4021 cttgtaaaaa ccttctatat aaaaacaatg ataaaaaaca tggataaaaa aagggagcaa
     4081 attgctccct tgatgatgac atgaactaaa tgcgtgattt atttcatgac acggtttcta
     4141 tattcacctg tacgcgtgtc aatctcgatt ttgtcgcctt gcgcacaaaa taatggcact
     4201 gagatttcat aacctgtggc gattttagct ggtttcatca ctttgcctga ggtgtcacct
     4261 tttactgcgg gttctgtgta agtaatttca cgcacaaccg ttgttggtag ttcaactgaa
     4321 atggctttgt cgttataaaa acgtatatct gccggcatgc cgtcttcaag gtagggaagt
     4381 acgtcttcca tgaactcagc atcaacatcg tattggttgt agtctgcatc cataaacaca
     4441 taagttgggt cggcaaagta tgagtaagtc gcttcttttt tctcaagcac aatcacgtca
     4501 aatttatctc cagcgccgta aatggcctca cttggggctt cagtcagcaa gtttttaaat
     4561 ttcattttta ccacagcagt gctacggcca gatttattgt attctgcttt taccaccact
     4621 aatggcacat cgcccaccat aataacgttg ccggcgcgaa gttcttgagc tgttttcatg
     4681 agattgcctt gatataaaaa agtactaaaa attgggttaa atattaaagc cgcgcattat
     4741 acctgaacgc ttattttttg caaataaatc accaacttag aagcaaggtc tggctgcgcg
     4801 gccagcgcca ttgtgcttgc ttgcgtaatt gcgcgaatcg cgggcaactg gcgcacataa
     4861 ttcagccaaa tttcagttga aatgctgcct tgcgcccagc caccgtttaa ttcggcaatg
     4921 gcctgaaacg tttcttcatg acaagcggtg tagaaaagtt tcaaaaacgc attgagtttg
     4981 accatatgcg tgttttctgt ttgaaaataa ggctgccaaa tcagtggttt tgctgcccaa
     5041 atgccccgca cccagctatc ttcaccacgc acaaaattga tgtcgcaagt ggctaacagc
     5101 gcgtcgtaat tgtcttgact taaaaatggc agcattttga tgtgtagttt gtcacgcaaa
     5161 tacgtttcgt gagggttact ttgtgtgatg cctaaaaaac tttcaacttg cggcaaaatg
     5221 ctgctatttg gcacatatac cacggtggat tgctggctgg cttgcagaat gtgcagtagg
     5281 tcttcaatgg gtgcgtttgg gtaggcaaat agggatattt ttaacgtttg ttctgctaga
     5341 tttgttctat gtgtgtcagc atacgacaca atattcggct cacgaattaa gccgccggtg
     5401 gcctcggtaa agccaggaaa gaaaaagtga cgcgatagcc cgctcgcttg cggtgagggt
     5461 agagcgtgaa attcatcaat ccacttttca gccgataaat actctaaatt cacccatttt
     5521 gtttgcggct gcattgcgct taaataccgt tgaggaatgc cgcaggcaaa ggtttcaatc
     5581 actacttctg caggctggat agcatcgtca tcattgggcc aagcccatat ttcaacatgg
     5641 ttgatgattt gtgtgccaag ttcaggttga taaccaggaa tgattttttt ggcggtctca
     5701 aggtcatcaa taaataatcg aatcggcagt tgatgcgtat tggccagttg ttgactgagg
     5761 cgccagcaaa cgccgatgtc gccgaagtta tcgacaatgc gacagaaaat atcccagcgc
     5821 tggtcggtgc cgttttgcat ggtgttcgat ttatttgggt aagttgtatt gaatgtcgat
     5881 gacttcgtat tcttcgtcac cggctggcgt tgagacttta atacgatcgc ccacactttt
     5941 gcccagcatg gctttggcta aaggtgaaac ccagctgata tagcctttgc ttggttcaag
     6001 ttcatctttg ccaacgatgc ggtagctgtg ctctgtatca tttttaagtg aaaacaagat
     6061 tacccaagcg ccaaagaaga ttttttctaa gccgacttgc gtggcggggt ccacaatgat
     6121 ggctaaatcc atgcgctgca ttaaaaaacg caggcgacta tcgatttgcc gcaagcgtcg
     6181 cttgccataa atataatcac cgttttcact gcggtcgccg ttgcctgccg cccacgacac
     6241 cacgcgtact acttctggcc gttctacttt aagtaattgc tgaaattccg cttcaagcgc
     6301 tgcatgaccc tcaggcgtga tgtagttttt ttcgtctgag acggctagtt ttgctttggc
     6361 catatttact gcgctgtttg cggattcatg ggttgcgtgt cgagcaactg ttgttctggc
     6421 caaacctgat caatgggctg tacatcatgc acaaaataac ctgtttcgcc aaagcaggtg
     6481 gttggaatac cgatatgctc ttcataaaca aggcgcacac gtttgccgac ggtttcattg
     6541 attttttttg ccacggcttt gtcgcgcacg gtgaagaaaa acttctctgc ttgtgtgcca
     6601 ggcattgaaa ccaatatcag ttcgccttca aaagttttac atacccagcc tttttctgat
     6661 agcttttgca cgtagcctgc gcgttcgcca gaagaataga cccaagtaag tgttgcccaa
     6721 gtgtataaag caaataggcc aattggcacc agtaatagcc aaatagaata tttaagcagt
     6781 tgcaagagtt ttgctaacat gaaaagtcct tattttaagt gcgtcatatt ttgtaagtgg
     6841 cgtcattatc gcgcgaattg ccactatttt attcggcttg ggtaaattat cctagcgtgg
     6901 caaataatag tgtaataatt attagcatgg taattatcaa gtcgctatag aatactagga
     6961 tgtcaaaaca aacgcttatt ctttgcccat cagcccgttt ggcgcgcagt attcaaaacg
     7021 atattgccct acaaaacagc cagtctggcc aagtcgtttg gcatagcccc gccgtaatga
     7081 cgctatcgca gtggttagaa aatgtcatta atactgcttt tttatcaggg caattcacgc
     7141 aggtgcagcc ttatgcctta agcacactca acgagcagtt actttggcaa gaggtgatta
     7201 cgcaatcttt aaagaaaaat gcgtttggtg cgttgtttga tgttgcgggt ttggcgcagg
     7261 ccgccatgga ggcgaaccgc tatcaagtag cgtggcactt gcatttgccg cgtgagcatt
     7321 tggctgaaga atcccgccag tttttgcact ggcaacgtgc atttgcggcg cgttgtgctg
     7381 aattaaatgt gcttgaacag gtgcgctatg tggattggca actcgcgcaa ttggccgcgg
     7441 gattcgtcat gttgccatcg cacatcgcct ttgctggttt tgaccaaacc gcaccacaag
     7501 aattgcgttt gcgtaaaatt ttagtgtgtc agggcgttga ggtttctgag tacgctacaa
     7561 ctgctgatac agtggctaat gcgcaacatg tgcgcttaga gaatcaagcg gcagagctac
     7621 gcgcagcggt ggcttgggct aaagatttgt tggcacacca gcccaaagca aaattagcga
     7681 ttgttgtgcc gcaacttaat accgtgcgaa accagctggc agatttgctg gatgatgtgt
     7741 tttacccaga aagtgtgcgc cctggtttat ttgaaatgcc gcgaaattac aatttttcgc
     7801 tcggcacgcc attagcacag cagccgatta ttcaagctgc gctgaatttg ctgcgtctat
     7861 tgagccacag ttcagccaat caacccatgc cgcaaagtga ctattcagca gtgctgttat
     7921 cgccattttg gtctgcaagt acaacagaag ccgatgcccg tgcgatgctg gatgcaacca
     7981 tgcgcgacaa attaccgatg cagtttaaac tcaccaactt gctggcattg gcagaaaaac
     8041 agcttaaaaa cggcttagcg attaccaaat ttttggccga tttaaaagcc gcaattgcac
     8101 tcatccccaa taaaaaagtg ccgccatcac aatgggcgca gaccatcacg acattattaa
     8161 ccgcattgca ttggccgggt gaacgcgctg ttaccagcat ggaatatcag gcacaaaatg
     8221 cttggcaaaa agccttgcag caattagcgc aaatggatgt gcttcaaaaa aatattgggc
     8281 tagcagaagc ggtgagtctg ttgcagcaaa tttgcacggc gatgattttt caggcagaaa
     8341 cagaatatgc accaacgata cagattttag gcgttatgga agctctgagc gcaccggtgg
     8401 atgcgctttg gtgcatgcac atgaacgacc acatctggcc gccgccagcg cgccccaacc
     8461 cgttattacc tgcgtatttg caacggcagg cgatgctgcc caatgccgat aacactgtgc
     8521 aggctgcttt cgccgagacg attcatcagc gattacttca ttccgcacca cagattattt
     8581 tttcaagcag catgtcagaa agagatagcc aactacgccc gtcaccactg atgaaaaata
     8641 tggcccatgc tgcgcatgaa atgccgctag caaacacgct ggccgagcaa ttaagcgctg
     8701 aaggcaatac cgatttaact tttttagatg accacattgc ccccgcaata caagccgatg
     8761 aacacgtgcg cggtggcaca ggtctattta aagcgcaggc agtttgcccc gcctgggcct
     8821 tttaccaata tcgcttgggc gctaaagcgt taaaaacccc aacggatggt attgatagca
     8881 tggaacgtgg ttcgctggtg catggcgtgc ttgagcagtt ttggcgcaag cgccattttg
     8941 cggatttgcg cgatatgtcg gtggatgcgc tttatcttgc ggtcaatcat gccatcacgc
     9001 agactttgca agtatttgcg gccgaaaatt cggtttcagc caatgtgctg gcgcttgagc
     9061 atgaacggct ttttaagttg ataacggatt ggctgcttta tgaaaaattg cgcggcgtag
     9121 gttttaacat ggtagcgtgc gaggccgaga aaaaagtcag catttgtggc attgaagtca
     9181 cgttaaaaat tgaccgcatt catgcgcttg aaaatggtgg gctggaattt gtggactata
     9241 aaactgggca agcacccaaa atcaccagtt gggcagaaac gcgcattacc gagccgcaac
     9301 tgcccatcta tgcggcattt tatatggaag atgctgaaca cgttacaggc gtacagtttg
     9361 gtatggttaa aacagcagag cacatgttta ctggcgtgag cgcagaaaat tttgacactg
     9421 aacctgaaaa acgcaagcca gtgtttattc gcgcattcag cgattgggca tcattactca
     9481 cgcactggaa aagcagcatt gaggccattg cccaagaaat taaaagtggc gaggcggctg
     9541 tgcgctttaa cgatgaacag gcattggcgt attgtgaagt gttaccacta ttgcgtttgc
     9601 ccgagcgaaa attgcagttt gagcgctttg caaataacgc agatgagggc gctgtatgat
     9661 gcaattttta gcgcaaaaaa acacgtgttt taaggcattt tttacctcca acctttttcg
     9721 atgctctgga agccttgtaa ataaaggctc gcagaggtat ttttttctct cggcgatttt
     9781 ttaagtgatt tttttcagtg attttaaagt tggatttttt aggtaaaaat gcatgacagc
     9841 acagtgtgaa ttgaatcttg caacggatga aaaaaaccgt cagcgtgcgc tcgatttaac
     9901 ctcatttatt gtagaggcgc cagcaggggc gggcaaaacc gaactactca cgcagcgtta
     9961 tttgaaactg ctgcaaacag tgagcgcgcc tgaagaaatt attgccatta cttttaccaa
    10021 taaagcagcc gcagaaatgc gtgtgcgtat tttagacagt ttaatcaagg ctagtcatcg
    10081 cgataaacca gccgccgcgc acaaacaaat cacatatgca ttgagtctta aagcccttga
    10141 aattgctgag gctaagcatt ggcagctcat cgaaaaccca gcgcgattac gcatttttac
    10201 cattgatagt ttgtgtgcgc atctggcgcg gcaaatgcct ttgatgagtc gttttggcgc
    10261 acagccgcgt gtcgcggagg atgccggggc actctacacg caagcggcag aacatacgct
    10321 ggctttgctt gaagcgcccg cgcataccga ggtggtgaaa tatgctttgc gttatgttga
    10381 taacaatgcc gagcaattaa aaggcttgct cgtaaaaatg ctcgaaaagc gcgaccagtg
    10441 gctgcatcac gcgcaacgcg aagtggatgt tggcgttttg cagcaaacat tacgacattt
    10501 ggtagagcaa gaattagcaa acatcgcaca agtttttaat gcgcgcctgc aatttgcgct
    10561 aatgccactt gcacgctttg ctgctagcca gttaccgtgc gaacacgcca ttgctttgtt
    10621 gcgtgattgg gatacggctt tgatgacgca tgccgaagcg ctacccatgt ggcaaagtgt
    10681 gcgtgaatta ttgctgacca atgatgatag tattcgtgcc agtatcagca aaaacattgg
    10741 ttttccgcca gaatttaaag atgaaaaagc gcaatttaaa tgctttttag aggctttgca
    10801 ggcggtgccg aatagtgcag aaagtttagc gaaagttaaa aaactgccaa acatagccaa
    10861 tacgcaagat gcctggcaaa tcatcagcac attgtctcaa ctgctcaatt tggcggtggc
    10921 tgaactctgg ttagtgtttc agcgtgcggg tgaggttgat tttgttgaaa ttgccacgcg
    10981 cgccacgcag gcacttagcc atgattttgg tgagcctacc gacttagcct tgaaattaga
    11041 ttaccagatt cagcatttac tggtggatga gtttcaggac accagcccca gccagattga
    11101 gttgctgaag ctactaacac tgggttggca agtgggtgat caccgcacgt tattttgtgt
    11161 gggcgacccc atgcagtcga tttaccgctt tcgtaaagcc aatgttgggc tgtttattaa
    11221 agcatcaacg caaggcattg gcggcatcaa ccttgaaaga ttgcagttgt atcaaaataa
    11281 tcggtcaagc ccagcggtgg tggattggat taaccagacg tttgaacatg tttttcctgc
    11341 agaagacgac atgatgcagt ctgctgttca ttaccgccca tttattgcca ccaaagaaaa
    11401 tttaaccaat gcgggcgtgc aggtacaccc cattattaaa cgcgcagatg aaagcaacgc
    11461 cttagcgcaa cagcgcgaag ctgaagcggt gatcaacatt attttgcaag cgcaagcagc
    11521 aaatgcacag caaaaaatcg cggtgctggt gcgctcaaaa aaacacttgg cgcaattggt
    11581 gcgcctgtta cgccgtcatc atcctgacat tgcttttcag gcggtggaga ttgaggcgct
    11641 ggaaggccgc cagattgtgc aggatttgct gtcactcacg catgctttgc atcaccgtgc
    11701 agaccgcgtg cattggttga gcctgttacg tgcgccttgg tgtggtttaa cgttgcatga
    11761 tttacacgcg ctggcggggc aaaatttaca cgccaccatt tggtcactca tgcaaaatgc
    11821 ggcggttgtg cagcggctat ctgccgatgg tcagaaacgc ttaatccatg tgcgtgaaat
    11881 tttggcagag gcctttgcaa cgcagggccg catgaatacc agccgctggc tgcgcggcgt
    11941 gtggttgatg ttaaatggca gcgcctgcct atgggcgcaa aatgatgtga ttgacgtaca
    12001 ggcttttttt gcctgtttag aagcgcttga ccgcagcaat cagttatcac ccgagcgcat
    12061 ggcgaatgaa attaccaagt tatttgccag cccggaagcg cagggcgagt gtttgcagat
    12121 gatgaccatt cataaatcga aaggtttgga atttgacacc gtgattctgc ccggtttagg
    12181 cgccaccacg ggcggcggca acggtgataa gccactgctg ctgtgggaag aagtgccgat
    12241 tgatgacgat acgcaattac ttgcggcacc ttatctaccc aaaaatcagc gcaatcacga
    12301 cagcgtcagc ccctacgact atttagaaat gttagaaagc acacgtgaga aaaatgaagc
    12361 cgcgcgtgtg ttatacgtgg cggccacgcg tgcggagcga tgcctgcatt tggttggcat
    12421 tgctaatcaa aaagcggata acagcattgc accaaccaaa aacacctatt tagatttact
    12481 atgggaacca ctggcagcgc attatgaaat gccagtattg cagcctgatg ctgctgcaaa
    12541 ctcagaagat gatattgcat tttttacgcc ac
//
LOCUS       DAOQSI010000047        12248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_4303_length_12248_cov_5.837530, whole genome shotgun sequence.
ACCESSION   DAOQSI010000047 DAOQSI010000000
VERSION     DAOQSI010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 12248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12248
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4303_length_12248_cov_5.837530"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            266..1699
                     /locus_tag="PKL42_04610"
     CDS             266..1699
                     /locus_tag="PKL42_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNU66390.1"
                     /translation="MAVPRPVLMIIDDDPLITDTLHFVLSKYFDVCVAESHAQLKSLL
                     LQQDAPPALALVDLGLPPSPHTPEEGFKVISALLAHSPTIKIHVLSGQSDDVNVKRAL
                     SLGAVDFIAKPCDVDKLKDMLLNALKVQDVELNEIKAEQEHDGLLGNSLPMQALRMQI
                     NQFADSPFPVLIEGESGSGKELVAGSFHHASKRRMHPYLSVNCAAISPLLAESILFGH
                     TKGAFTGAISAHTGYFEDASEGTLFLDEIGELPLELQAKLLRVLENGEYQRIGETQTR
                     KSRARIVAATNRDLRLEVRAGKFRSDLYHRLSVFAVHVPPLRELGEDKALLREHFISF
                     YAKQIGQAPFTLDASAQARWLEYDFPGNVRELRNIVIRLIAKYAGKTLNANDLVAEFD
                     LSHAAPEGSSTEQININDESAMMAQAQRHLQTQANVTLDAVLKTWEKAYIEAAMKITH
                     GNLSQAAKLLNVNRTTLYSRMSTPENS"
     gene            1717..3096
                     /locus_tag="PKL42_04615"
     CDS             1717..3096
                     /locus_tag="PKL42_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNU66391.1"
                     /translation="MYYPHFGLKEPPFKITPNTDFFFSGGNRGAVLDALVYAITNGEG
                     IIKVVGEVGSGKTMLCRMLQTILPEKIESIYLANPSVAPEDVLHAIAFELQLKLPKNA
                     DRLKVMQALQAHLLSRHKEGRQVVIFVEEAQGMPLATLEEIRLLSNLETKQDKLLQIV
                     LFGQPELDTNLNQPHIRQLRERITHSFHLGPLQTKDIGEYLIFRLRAAGYYGPHLFTD
                     EAIKKLSAAAQGLVRRVNILAEKSLLAAYAENVHQVSVKHVNAAIQDSEFGKSASATK
                     KLLSSQAILIGVAIIALFAALTLFFLRQNNPTQPQEKTALIFNQPANITNQKAPESST
                     NTSKKASVAQQVEPKQNLIDSRLEATKTLLRDGHPDSITLQMMTSSDERQVKEDLDAL
                     SRQLEVDKLYLYQKKQNSTNLTVFLYGIFSQRAEAISAMQGLPSHIKNNRPQLRTFAG
                     INKELEQSQ"
     gene            3207..5000
                     /gene="mshL"
                     /locus_tag="PKL42_04620"
     CDS             3207..5000
                     /gene="mshL"
                     /locus_tag="PKL42_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus (MSHA type) biogenesis protein MshL"
                     /protein_id="HNU66392.1"
                     /translation="MTNNIRITLLACVALALASCAHESKVQPSSGHIDGQNDSASQPN
                     VAVTNVADIPKPITKNTYLPPPKAKAKEPVYSIVVYDTPVREVLFAIARDSKFNVDIH
                     PNIKGNVTLNAVDQTLPAILERISKQVDLTYKIQNNVLTIAPDQPVLRTYKVDYVNMS
                     RDTTSFIGADGQISSASQSSGANGTISNSAGNGANNSSRTSVTSESKSHFWETLEKNL
                     KDILAETDKEVLITRSGSSSKSNTKSKAGQAGKSAEDEVANVADLKSEREEDRNEFKT
                     LFAGTVIVNREAGIINVRATNKQHEKIQDFIDKVMSSARRQVLIEATIVEVRLNDSFQ
                     AGIDWSRFGNTATSSGFTFQQSLGPSINPASNGFAIGYLNPVSSLGNLAASVTLLKQF
                     GDTKVISSPKLMVLNNQTAILKVVDNIVYFTVQAQQSQATQGGVLSTVTTTPNTVPVG
                     IVMSVTPQINENSAVNINVRPTISRVVSFVRDPNPQLANIASQVPQIQVREMESLLQV
                     NSGNTAILGGLMQDEIQRNTDKVPGLSDIKGFGEIFKGRDYANKKTELVIFLRPTVVK
                     NASLESDELSRYKQYLPSSQLQQTLEVDGER"
     gene            5009..6313
                     /locus_tag="PKL42_04625"
     CDS             5009..6313
                     /locus_tag="PKL42_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNU66393.1"
                     /translation="MSLLIKALANAEKDKQAELDKKKSVERDSALSLELAPIDQAQNS
                     NKSRTPRQSFEKAPIHSDNELKHQEAAKVFVANQAASTPKSNLNLLLIGVAGAAIILL
                     GFQGYVYIQSLSSPQIASAKVSVPAVQPIPDQQVQIEETSTQSQTELNTQAVAINSDE
                     PEKTQDETLAEKQSEPDSFSVDNDELNKASPKPVKKSNLKANVSNTVNNSESSIDNSQ
                     QQVRGKPSKQAPLKLTTKSPAAGVDPTLLAAYEALNRGEYAIAQQQYRAVLQKDVRNV
                     DALLGMAVIAQRQTRDADAEGWFQKVLEVEPRNVIAQTALMGVKEGGGGSDRLEQNSE
                     SHIKSMLAQQPEAANLHAALGNVYAEQGQWTSAQSAYFEASKLAPHNADYAFNLAVSL
                     EQMRKPHLALEQYQRALALLNQSGATSPDRLTLETRIQALQK"
     gene            6352..8088
                     /locus_tag="PKL42_04630"
     CDS             6352..8088
                     /locus_tag="PKL42_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNU66394.1"
                     /translation="MAEQRRILRLGELMVQQGLISQDQLRIALIEQEQNDLPLGRQLV
                     RLGFVTEAMVRDTVAHTIGQESVDLSAVVADIEALQMVPQDFCRRYHVLPVAFEESSK
                     SMVVAMADLFNVVALDQLRAMLGGQIQLKPVLAAEAQLEEFIDQFYGYELSVDGILRE
                     IETGEIDYQSLTAGGDEYTQPVVRLVGALLMDAVKRGASDIHFEPEYAFLRIRYRIDG
                     VLQQIRSLHKSYWGGLAVRLKVVSGLDIAETRAPQDGRLNMNLCGRPIDFRVSTHPTI
                     HGENIVLRVLDREKSIIPMERMGLRPTTLDEMRLMMTRPEGILIVTGPTGSGKTTTLY
                     SLLSHRNTEAVNIMTLEDPVEYPVTMMRQTSVAEVNKVDFANGIRSIMRQDPDIILVG
                     EIRDTDTATMAFRAAMTGHQVFSTLHTNSALGAFPRLADIGILPDIMAGNIIGVVAQR
                     LVRVLCPHCKEVHKLDSLERKILRLKTTDKPQIYKSKGCKLCNQTGYRGRMAIIELLR
                     IDSDFDSLISRRAHLDELQKLALDKGFVTLAEDGVRRILEGYTSLAEVMRVIDLTSRM
                     HDGVKTKVASKS"
     gene            8092..9297
                     /locus_tag="PKL42_04635"
     CDS             8092..9297
                     /locus_tag="PKL42_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNU66395.1"
                     /translation="MAAYNYKAIDQMGRPAMGQIDAINEIDLEIRLERMGLDLITFRA
                     AKKSTTLFNRNKVSNQDLVMFCFQMEQLSSAGVPLLESLNDIRENNPNLYFQKILGAV
                     SAEVEGGKMLSQALAEHQSVFGEVFVSLIHAGEQTGQLPVVFNNLFETIRWQDELMSQ
                     TKKLLAYPAFVAVVVLGAVAFLMSYLVPQMVSFLRNMGQELPLNTKILIMFSNAFVHY
                     WWLMIGLPIIIFIAATAIIKTNPVARYRFDLFKLNMPITGPILHKIIMARFARYFALM
                     YQTGIPILDAIKICEKIVGNRVVTDALSRVHAQINSGDAMSESFRNAGLFPPLVVRMI
                     RVGENTGALDKSLLNISYFYDRDVKDSMEKMLKLIEPTLTVILGAILGFIMFSVLGPV
                     YDSFSKLNF"
     gene            9331..10986
                     /locus_tag="PKL42_04640"
     CDS             9331..10986
                     /locus_tag="PKL42_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66396.1"
                     /translation="MFKASQHKIVLCATANNLLAGVWRNGQLQGNQAFANDDVGYVAF
                     TEFLKQYESAPIYLIADAVEEDYKLESLPHTSGAAKRELIERKLNQFYRGLAYRTAHF
                     IHREKDKRKDDKYLFVALSNDEFLKNWVALIHAAKAQLVGVYLLPMLSQVLVRQLKLM
                     SPHILLCEKLSSGLRQTYFHNGRLRMSRLVTSVPTAANQLGYFYLVETEKTRLYLISQ
                     RFISQDTPINLVLVSIDGSTKAISQGISQEQTLECTDLDLSQTAKSLNLPAHLVQQMP
                     ELLHMQLLAKGHLVDNLAPSSQTKEYQFNQVKRAIFIATALIGLIGLLVAGWFFKLGL
                     DNKEAFNQAVQDTVIQEHRYSEAAKNFPATPVSAADLKLAVELDQSIAAYPKSPRRMM
                     LVVSAALELSSEIQLDRLRWAFTNDLNIKDEDNLLALPASNLQPTASVMDDPAKLIEL
                     VYVNAQISNFNGDYRAALNSVNKFVANLKANKQVATVEVLQAPVNVSSYVGLQGSTTD
                     EQSTQRQPALFKLKVILKSADALVETPLVEQVPSNQALGKQLP"
     gene            10983..11636
                     /locus_tag="PKL42_04645"
     CDS             10983..11636
                     /locus_tag="PKL42_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66397.1"
                     /translation="MKLEATKQEARPSAKDAHKLQVPILLIGITLIVVIILIVLAHRY
                     STEQEKALQTQQGLLNTARQHFQSSGIEKDMITEYLPKYQMLIDKGFVGEERRIEWVD
                     ELRAQHKNHKLFGVKYSISQQEVYKPAFISNTGGFVLHRSVMTLDLDMLHEGDILQLI
                     ESLGAKNGAPFVLRDCEIRRLNANTELSRQLVANLHAQCELDWLTLHEPLAQPATNP"
     gene            11633..12184
                     /locus_tag="PKL42_04650"
     CDS             11633..12184
                     /locus_tag="PKL42_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66398.1"
                     /translation="MTSMMNYLFTFLLLLGLAIPKVHAAPGDNLGRLFSRPMERNNLD
                     VLRQNQKLKVIVPQENTAPEIEEKAAPEELPDPITLQGYVKRSDGKGNTIWINNQAVQ
                     ENSTVDKVKVGKVNQRGFSKKGASTDGVDIKIPMTGKQVRLKPGQMYAPENNKIYEMQ
                     VVEKSKRLELEQSGVIDGDETPQ"
BASE COUNT         3733 a         2416 c         2852 g         3247 t
ORIGIN      
        1 gaatggatcc aattgagggt agcacttacg gtagcactta tttttgggat ttgtacgcta
       61 caataggctc gtacacattg attaacgctg gcttgtgaaa attatgggtt cgagccccat
      121 cagccacccc aaattttctc cagcaatcaa aacacattta atcgtgagcg cgaaggcggc
      181 ccgtattcgt caaactcatt gatacgcgta gcctttaagg ctatagtatc aacgattaca
      241 ctttaatttt tgtaaaggtt tttgaatggc tgtacctcgt ccagttttaa tgattattga
      301 tgatgatcct ttaattacgg atacgttgca ttttgtttta agtaaatact ttgatgtttg
      361 tgtggctgaa tcgcacgcgc agctcaaaag tttactgttg cagcaagatg ccccgccagc
      421 cttggcgtta gtagatttag ggctgccacc atcaccgcat acgcctgaag agggctttaa
      481 agtcatcagc gcactattag cgcattcccc taccattaaa attcatgtgc tttcgggcca
      541 aagtgatgat gtcaatgtga agcgcgcatt gtcattgggc gcggtggact ttattgccaa
      601 gccttgcgat gttgataagc ttaaagacat gctcttaaac gcacttaaag tgcaagatgt
      661 tgagctaaat gaaattaaag ccgaacagga gcatgacggc ctgttgggga acagtttgcc
      721 aatgcaagcc ttgcgtatgc aaattaacca atttgcagac tcgccttttc cagtactgat
      781 tgagggtgaa tctggcagtg gtaaagagtt ggttgcaggg agctttcacc acgcaagtaa
      841 acgtagaatg catccttatt tgtcggtcaa ttgcgccgca atttcgcctt tattggctga
      901 gtctatttta tttggccata ctaaaggtgc gtttaccggt gctatttctg cgcatacagg
      961 gtattttgaa gacgcgagcg aaggcacgct gtttttagat gaaattggcg aactgccatt
     1021 agaattgcag gcaaagttgc tacgcgtatt agaaaatggt gaataccagc gaattggtga
     1081 aacacaaact aggaagagtc gcgcacgtat tgtagcggca actaatcgtg acctgcgact
     1141 tgaagtgcgt gcaggtaaat ttagatcaga cttatatcat cgtttaagtg tatttgctgt
     1201 tcacgtgccg ccacttagag agttgggcga agataaagcg ttattgcgag aacactttat
     1261 ttcgttctac gccaaacaaa ttgggcaagc gcccttcacg cttgatgcct cagcgcaggc
     1321 acgctggctt gaatatgatt tccctggcaa tgtccgcgag ctcagaaata tagtgattcg
     1381 gttgattgcg aaatatgctg gcaaaacgct taatgctaat gatttggttg ctgagtttga
     1441 tttgtctcac gccgcgcctg aaggctcaag tacagagcaa atcaacatta acgatgagtc
     1501 tgcaatgatg gcgcaggcgc agcgccactt gcaaacacaa gcaaacgtga ccttggatgc
     1561 cgtacttaaa acttgggaaa aagcctatat tgaagcggcc atgaaaatta cgcatggcaa
     1621 cttaagtcag gctgcaaagt tgttaaatgt aaatcgcacc acgttatata gccgcatgag
     1681 cacccctgag aatagctgac aggtactgga tacgctgtgt attacccgca ctttggctta
     1741 aaagaaccac catttaaaat tacccccaac actgattttt tcttttctgg gggtaatcgg
     1801 ggcgcggtat tagacgcact cgtctatgct attaccaacg gcgaaggcat tatcaaggtt
     1861 gtgggcgagg ttggcagtgg taaaaccatg ctttgccgca tgctgcaaac cattttgcct
     1921 gaaaaaattg agagcattta tctcgctaat ccaagtgttg cccctgaaga tgtgttgcac
     1981 gccattgcat ttgagttgca actcaaactc cccaaaaacg ccgaccgcct caaggtcatg
     2041 caggcactgc aggcacattt attaagccga cacaaagaag ggcgccaagt cgttatattt
     2101 gttgaagaag cacaaggcat gccgctggca acgcttgaag aaatcaggct actttcaaac
     2161 ttagaaacta agcaagacaa actgctgcaa attgtcttat ttgggcaacc agaactcgat
     2221 acgaatttaa atcagccgca cattcgtcaa ctgcgtgagc ggattaccca tagctttcat
     2281 cttgggccac tacaaacgaa agatattggc gaatatctta tttttcgctt gcgtgcggca
     2341 ggctattacg gcccacattt atttacagat gaagccatta aaaaactctc agccgcggcc
     2401 caaggtttgg tgcggcgcgt aaatattctg gcagaaaaaa gtttgttggc ggcatatgca
     2461 gaaaatgtgc atcaagtgag tgtaaagcac gtcaatgccg cgattcaaga tagtgaattt
     2521 ggcaaaagcg caagcgctac gaaaaagtta ttatcgtccc aagctatatt aatcggcgtg
     2581 gcaattatcg cgctattcgc agcattgacc ttattttttc tacgccagaa taacccaacg
     2641 cagccgcaag aaaaaactgc gcttatattc aatcagccag caaatataac aaaccagaaa
     2701 gcgcctgaaa gtagcaccaa taccagcaag aaagccagcg tagcgcagca ggtagagcct
     2761 aagcaaaatt taatagatag ccgattggaa gccactaaaa cactgctgcg agatggccat
     2821 ccggatagta ttaccttgca gatgatgacc tcctctgacg agcgtcaggt gaaagaagat
     2881 ttagatgcat tgagtcggca acttgaagtg gacaaactgt atttgtacca aaaaaagcaa
     2941 aatagcacca accttaccgt gtttttatat ggcatttttt ctcaacgtgc agaagcgata
     3001 tccgcaatgc aaggcttgcc tagtcacatc aaaaataatc gcccccaatt gcgtacattt
     3061 gctggaatca acaaagagct tgagcaatcg caatagaaaa cccctgtaaa ttattgtttt
     3121 actttggtat tttgtataaa atacttcttg catttttgca atagtgtgca aaaatacagt
     3181 aatgtaaaaa ataaggggta aatcctttga caaataatat tcgaattacg ctactggcgt
     3241 gcgtagcctt ggcattagca tcgtgcgcgc atgagtcaaa agtccagcct tcaagtggtc
     3301 atattgatgg tcaaaatgat agcgcaagcc agccaaacgt agctgtcacc aatgtggctg
     3361 acatcccaaa accaattaca aaaaacacct atctgccccc gccaaaagca aaggcaaaag
     3421 aacctgttta cagcattgtg gtgtacgaca caccggtaag agaagtgttg tttgccattg
     3481 cgcgagatag caagtttaat gtggatattc atccaaatat taaaggaaat gtgaccctaa
     3541 atgcagtgga tcaaacatta ccagcaattt tagagcgtat ctctaaacaa gtcgatttaa
     3601 cctacaaaat tcaaaataat gtactgacga ttgcgccaga ccagccggtg ttgcggacgt
     3661 ataaagtgga ctatgtcaac atgtcgcgtg acaccacgag ttttattggg gctgatggac
     3721 aaatttcgag cgcttcgcaa agttcagggg caaatggcac aatcagtaac agtgcgggca
     3781 atggggcgaa taatagctcg cgtacttcag tgaccagcga gtcaaaaagc cacttttggg
     3841 aaacgttaga aaaaaatctc aaagatattt tggctgaaac tgataaagaa gttctcatca
     3901 cccgttctgg tagttcaagt aaatcaaata cgaagtcaaa ggccggccag gcaggtaaaa
     3961 gtgcagaaga tgaagtagcg aatgtagcgg atttaaaaag tgagcgtgaa gaagaccgta
     4021 acgaatttaa aaccctgttt gctggtactg taattgtgaa tcgtgaggca ggcattatca
     4081 atgtgcgtgc tacaaataaa cagcatgaaa agattcaaga ctttattgat aaagtcatgt
     4141 ccagtgcgcg tcgtcaagta ctgatagagg caacgattgt cgaagtgcgg ttaaatgata
     4201 gttttcaggc ggggattgat tggagccgtt ttggcaatac cgcgaccagc agtggtttta
     4261 cctttcagca atccttaggg cctagcatta accctgccag caatggtttt gcaatagggt
     4321 atttaaatcc ggtgagctcc cttggaaacc tcgctgcctc cgtaacttta ctgaaacagt
     4381 ttggtgacac aaaggtgatt tctagtccaa aattgatggt gctaaataat caaaccgcca
     4441 ttttaaaagt agtagataac attgtatatt ttaccgtgca agcgcagcag tcgcaggcta
     4501 cgcaaggtgg cgtactatct accgtaacca ccactccaaa taccgttcca gtgggaattg
     4561 tgatgagtgt gacgccacaa attaatgaaa acagcgcggt gaacattaat gtgcgcccaa
     4621 ccatttctcg cgtggtgagt tttgtgcgtg accccaaccc gcagttagcg aatatcgcta
     4681 gccaagttcc acaaattcaa gtgcgagaga tggagtctct tttgcaggta aatagtggta
     4741 atacagcaat attaggcggc ttgatgcaag atgagataca gcgtaacacc gataaagtgc
     4801 caggcttgtc agacattaaa gggtttggtg aaatttttaa aggacgtgat tatgcgaata
     4861 aaaaaactga gttggtgatt ttcttacgcc caacagttgt gaaaaatgca agcttagaaa
     4921 gcgatgaact ctctcgctat aaacaatatc tgccaagttc acagttgcaa caaacgcttg
     4981 aagttgatgg tgaaaggtaa gttgcgcaat gagtttactc attaaggctt tggcgaatgc
     5041 tgaaaaagac aagcaggcgg aactcgataa aaagaagtca gtcgagcgtg attccgcact
     5101 atctctagag ttggccccta tcgatcaagc gcaaaatagc aataaaagta gaacacctcg
     5161 tcaatcattt gagaaagcgc caattcatag tgataatgaa ttaaagcatc aagaggcggc
     5221 aaaagtgttt gtggccaacc aggcagcaag tacgccaaaa tcaaatttaa atttattgtt
     5281 aattggtgtc gcaggggcag ccattatttt gttaggcttt cagggatacg tctacataca
     5341 aagtttatcc tcaccacaaa tagcatcagc aaaagtaagt gtacctgccg tgcaacccat
     5401 cccggaccag caagttcaga ttgaagagac ttccacacaa agtcagactg aactcaatac
     5461 gcaggctgtt gcaattaact cagacgaacc tgaaaaaacg caagatgaaa ctttggcaga
     5521 aaagcagtcg gagcctgatt cattttctgt tgataatgat gaacttaata aagcaagccc
     5581 aaagccagtt aaaaaaagta atttaaaagc aaatgtatcc aacacggtta ataatagtga
     5641 atcttctatt gataactcac agcaacaagt tcgcggcaag ccctcaaaac aagcgccgtt
     5701 aaagttaacc actaaatcac cagctgcagg ggttgaccca acattattgg cggcttatga
     5761 agcacttaat cgaggggaat atgcgatcgc acagcaacaa tatcgagcgg tgttgcaaaa
     5821 agatgtgcgt aacgtggatg ctttacttgg catggcagtg attgcacaac ggcaaactag
     5881 agatgcggat gctgaaggct ggtttcaaaa agtgctagag gttgaaccaa gaaacgttat
     5941 tgcccagacc gcgctgatgg gtgtgaaaga aggtggcggt ggaagcgata ggcttgagca
     6001 gaactcagaa agtcatatta aaagtatgct tgctcaacag ccagaagcag ccaatttgca
     6061 tgccgcttta ggcaatgtat atgctgagca gggccaatgg acatctgccc aaagtgctta
     6121 tttcgaggca agcaagttag cgcctcataa tgcagattac gcatttaatt tagcggtgag
     6181 tctagaacaa atgagaaagc cacacttagc tttagagcag tatcagcgtg cgttggcttt
     6241 gttaaatcaa tcaggggcta ccagcccaga tagactcact ctagaaacga gaattcaagc
     6301 actacaaaaa tgacaatatt aaatttaaaa ataataaagc aggttaaata gatggctgag
     6361 cagcgacgca tactccgcct aggcgaactc atggtgcagc aaggcttgat tagtcaagat
     6421 cagctacgca ttgcactcat agaacaagag caaaatgacc taccattagg ccgccaactg
     6481 gtgcgcttag gttttgtgac ggaagcaatg gtgcgtgata cggtggcgca tactattggc
     6541 caagaaagcg tagatctatc agcggtggta gcagatattg aagccttgca aatggtgcct
     6601 caagattttt gtcgccgata tcatgtacta cccgttgcat ttgaggaatc aagcaaatcc
     6661 atggtggtcg ccatggcaga tttattcaat gtcgtggcgt tagaccaatt acgtgcaatg
     6721 ctcggcggcc agattcagct aaagcctgtt ttggcggccg aagcgcaact tgaagagttt
     6781 attgatcagt tttatggata tgaactctct gttgatggca ttctgcgtga aattgaaaca
     6841 ggtgaaattg attatcaaag tttgactgcg ggtggtgatg aatacacgca gcctgtcgta
     6901 cgcctggttg gggcgttgtt gatggatgca gtaaagcgcg gcgcatccga tattcacttt
     6961 gaaccagagt atgctttttt gcgtattcgt tacagaatcg atggcgtgct tcagcaaata
     7021 cgcagcttgc ataaaagcta ttggggtggt ttagcagtaa ggcttaaggt tgtgagcggt
     7081 cttgatattg ctgaaacacg agcgcctcaa gatggccgtt tgaacatgaa tttgtgtggg
     7141 cgtccaattg attttagggt ttctacgcac ccaacgatac acggtgaaaa tatcgtttta
     7201 cgtgtgctcg atcgtgaaaa atcgattatt ccaatggagc gcatgggtct gcgacctaca
     7261 acgttagacg aaatgcgctt gatgatgacg aggccagagg gcatattaat tgttaccggc
     7321 cctacaggta gtggtaaaac aaccacgcta tattcactgc tatcgcatag aaataccgag
     7381 gcggtaaaca ttatgacact ggaagaccca gtggaatacc ctgtcacgat gatgcgtcaa
     7441 acttcagtgg cagaggtcaa taaagtagat tttgccaatg gtatccgatc cattatgcgg
     7501 caagaccctg acattatatt agtcggcgag attcgtgata ctgatactgc gacgatggct
     7561 tttcgtgcag ccatgactgg gcatcaagtt tttagtacgt tacacactaa ctctgcttta
     7621 ggcgcttttc cgcgtttagc cgatataggc attctgccag acattatggc gggcaatatc
     7681 attggcgtgg tagcgcagcg cttggttcgc gtgttgtgtc cgcattgtaa agaagtacac
     7741 aaactcgata gcttagaacg gaaaatatta cggcttaaaa cgactgataa gccacaaatt
     7801 tacaagtcaa agggttgcaa gctttgtaat caaaccggat atcgcggtcg gatggcaatt
     7861 attgaattgt tgcgtattga cagtgatttt gattcgttaa tttcgcgtcg cgcgcattta
     7921 gatgaattgc aaaaactagc cttagataag ggttttgtca cacttgccga agatggcgtt
     7981 cgtagaattc tagaaggtta tacaagtttg gccgaggtga tgcgcgtgat tgacctgaca
     8041 agtcgtatgc atgatggcgt caaaactaag gtagctagca agtcataggg tatggctgcg
     8101 tataattata aagcgataga ccaaatgggt cgcccagcca tggggcaaat tgacgcaatc
     8161 aatgaaattg acctagaaat ccgtctagag cgtatgggtt tggatttaat tacatttaga
     8221 gcggcaaaaa aatcgaccac cctttttaac cgcaacaaag tgagcaatca agatttagtg
     8281 atgttttgtt ttcagatgga gcaactctca agtgctggag ttccattatt agaaagcctg
     8341 aatgacatta gagaaaataa tcctaatctg tattttcaaa aaatattggg tgcagtaagt
     8401 gctgaagttg aaggcggaaa aatgctttct caggcgttgg cagaacatca aagtgtattt
     8461 ggtgaggtgt tcgtgagttt aatccacgca ggcgaacaaa ccggccaatt gccagtagta
     8521 tttaacaact tatttgaaac aattcgttgg caagatgaat taatgtctca aacaaagaaa
     8581 ctacttgcat accctgcttt tgtggcggtg gttgtattgg gcgcggtcgc ttttctgatg
     8641 tcctatttag taccgcaaat ggtttctttt ttgcgtaata tggggcaaga gttaccatta
     8701 aatacaaaaa ttttaattat gttttcaaat gcgtttgtgc attattggtg gttgatgatt
     8761 gggttaccta ttattatttt tattgccgcg accgctatca ttaaaaccaa cccagtggcg
     8821 cgttatcgat ttgatttgtt taagctgaac atgcctatta cagggccaat tttacataaa
     8881 attattatgg cgcgatttgc acgttacttt gccttaatgt atcaaacggg catcccaatt
     8941 ttggatgcca ttaaaatatg tgaaaaaatt gtgggtaatc gtgtggtgac tgacgcactg
     9001 agtcgtgtgc atgcacaaat taattcgggc gatgcgatgt ctgagagttt tcggaatgca
     9061 ggattatttc ctcctctggt ggttcgtatg attcgcgtgg gtgaaaatac tggcgcgtta
     9121 gataagtcat tgttgaatat cagctatttt tatgacagag atgtaaaaga ctcgatggaa
     9181 aaaatgctta aattaattga gccaacgctc accgtgatat taggtgcaat acttggattt
     9241 attatgttct cggtattagg gccagtttat gactcattca gtaaacttaa cttttaaagc
     9301 gtcgagcatc agttaaccag aaatcaaaac atgtttaaag ctagtcaaca caaaatagta
     9361 ctatgcgcaa cagccaacaa tttgctagct ggggtttggc gcaacggtca attgcagggt
     9421 aatcaggcgt ttgcaaacga cgatgttggg tatgtagcat ttacggagtt tttaaagcaa
     9481 tacgaatcag cacccattta tttgattgca gatgccgttg aagaagatta taagttagaa
     9541 agtttgcctc atacttctgg tgcggcaaaa cgtgaattaa ttgagcgtaa actgaatcag
     9601 ttttatcgag gtctggctta tcgtactgcg cattttatcc atcgagaaaa agacaagcgc
     9661 aaagatgata agtatttatt tgttgcatta agtaatgatg aatttttaaa aaactgggtt
     9721 gcgctgatac acgctgccaa agcccagctg gttggtgttt acctgctacc aatgctaagt
     9781 caggtattgg tgcgccaatt aaaactgatg tccccgcaca ttttgctatg tgaaaaactt
     9841 tcttcaggct tgcggcaaac atattttcat aacggtcggc ttcgtatgag ccgcctcgta
     9901 acgagcgttc caacagcagc aaatcaactg ggctattttt atttagtaga aactgaaaaa
     9961 acacgtttat atttaattag ccaacgattt atttcgcaag atacgccaat caacctagtg
    10021 ttggtaagta tagacggcag cacaaaagca attagccaag ggataagtca agagcaaacg
    10081 cttgagtgta cagatctcga tttgagtcaa actgcgaaga gcttaaattt accagcacat
    10141 ttagtgcaac aaatgccaga actcttgcat atgcaactat tggcaaaggg tcatttagtt
    10201 gataatttgg caccatcttc tcagacaaaa gaatatcagt ttaatcaagt taaaagggct
    10261 attttcatcg ccactgcttt gattgggttg attggtttat tggtggcagg atggtttttt
    10321 aaactaggat tagacaataa agaagcgttt aaccaagcgg tacaagatac tgttattcaa
    10381 gagcatcgtt atagtgaagc ggcaaaaaac tttccagcaa cacccgtcag tgcagcggat
    10441 ttaaaactcg ctgtagaatt agatcaatca atcgcagctt atccaaaatc accgcggcgc
    10501 atgatgttag tggtgagtgc tgcattagag ctttcatctg aaattcaact tgatagattg
    10561 cgttgggcgt tcacgaatga tttaaatata aaagatgaag ataatttgtt ggctttgcct
    10621 gccagcaatt tgcaaccgac tgccagtgtg atggatgatc cggctaaact gattgaactg
    10681 gtctacgtga atgcacaaat atctaatttt aacggcgatt accgcgcagc gctcaatagt
    10741 gtaaataagt ttgtcgccaa tttaaaagcg aataagcagg tggctacagt tgaagtgttg
    10801 caggcaccgg taaatgttag ttcttatgta ggcttgcagg gcagcacaac cgacgagcaa
    10861 tcgacgcaaa ggcaacccgc tttatttaaa cttaaagtaa tacttaaatc agctgacgcg
    10921 cttgtggaaa cgcctctagt agagcaagtg cccagtaatc aggctttagg aaaacagctg
    10981 ccatgaagtt agaagcaact aagcaagagg ctaggccaag tgctaaagat gcacacaaac
    11041 tacaggtgcc gatattgctg attggcatca cgctcattgt ggtgattatt ttaattgttt
    11101 tggcgcatcg atatagcact gaacaggaaa aagccttgca gacacagcaa gggttgctga
    11161 ataccgcgcg tcaacatttt caatcttctg gcattgaaaa agacatgatt acagaatatt
    11221 tgccaaagta tcaaatgctg atcgataaag gatttgtggg cgaagagcga cgcattgaat
    11281 gggtagatga gttacgagca cagcataaaa atcacaaact ttttggtgtt aaatatagca
    11341 ttagccagca agaggtttat aagcctgcgt ttatttcaaa tactggcggg tttgtattac
    11401 accgttctgt catgacgcta gatttagaca tgctgcatga aggtgacatc ttgcaattaa
    11461 tcgaaagttt aggggccaaa aatggcgcgc catttgtgtt acgtgattgt gaaataagaa
    11521 gattaaatgc aaatacagag cttagtcgac aactggttgc taacttacac gcgcaatgtg
    11581 agctcgactg gctgacttta catgaaccct tggcccaacc ggcaacaaac ccatgactag
    11641 catgatgaat tatttattta cttttctttt attgcttggt ttagccatac caaaagttca
    11701 tgccgccccc ggtgataact tgggacggtt atttagtcgc ccgatggagc gcaacaatct
    11761 agatgtgtta aggcaaaatc aaaaattaaa agtgattgtg ccgcaagaaa atacagcgcc
    11821 agaaattgaa gagaaagcgg caccagaaga gctaccagac cccatcaccc tgcaagggta
    11881 tgtgaagcgc agtgatggca aaggtaacac aatatggatt aacaatcagg cagtgcagga
    11941 aaatagtacg gttgataaag ttaaagttgg caaagttaac cagcgcgggt tttcaaaaaa
    12001 aggtgcaagt accgacggcg tggatataaa aattccgatg acaggaaaac aagtaagatt
    12061 gaaaccaggc caaatgtacg cgccagaaaa taacaagatt tatgaaatgc aggttgtaga
    12121 aaaatccaaa agactcgagc tagagcaaag tggtgttata gatggcgatg aaacaccaca
    12181 gtaatatcaa attaaaccaa cgaggggcat cgttaattat tttggtgttg attttagttc
    12241 tgggtggg
//
LOCUS       DAOQSI010000048        12195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_4338_length_12195_cov_5.185338, whole genome shotgun sequence.
ACCESSION   DAOQSI010000048 DAOQSI010000000
VERSION     DAOQSI010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 12195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12195
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4338_length_12195_cov_5.185338"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /gene="murD"
                     /locus_tag="PKL42_04655"
     CDS             complement(<1..1194)
                     /gene="murD"
                     /locus_tag="PKL42_04655"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNU66399.1"
                     /translation="MMVLKDKNVLVVGLGDTGLSALRWLECQGARLSVADSRENPPNL
                     DKVKAEFPALKVFTGTFTAALLRSVDLIVLSPGVSLNEPEIKAAIACGVSVVGDVELF
                     AQYRNRNAKVIAITGANGKTTVTTLVGEVCRAAGLKTIVAGNIGLPVLDALMMDVPDV
                     YVLELSSFQLETTDTLYLDAATMLNLSEDHMDRYAAMSDYAIAKARIFYNAKLQVLNR
                     DDAWSMLMARPKLAQVTFGLSEPDEGEYGLRLTNGETWLCFGEKELINMRDLKLFGLH
                     NAANALAAVALCRGIGIDFAPIIQTLYNFKGLPHRVEWIANIDDVDYYDDSKGTNVGA
                     TCAALGGLPQKVVLIAGGDGKGQDFSPLKDAVQSNARAVVLIGRDAQLIEAVLHSTGV
                     PIYRAM"
     gene            complement(1198..2307)
                     /gene="mraY"
                     /locus_tag="PKL42_04660"
     CDS             complement(1198..2307)
                     /gene="mraY"
                     /locus_tag="PKL42_04660"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNU66400.1"
                     /translation="MLLDLAQWLAKVQHIRGFSVFDYITLRAVLATLTSLAISLMVGP
                     SMIRKLTEYKVGHAVRDDGPQTHLIKSGTPTMGGALILVAIAISTLLWGDLSNRFIWV
                     VLFTTLGFGAVGWVDDYRKVVYKNPKGLSAKAKYFWQSLIGVAVAVFLYKTAKTPVET
                     TLIVPFFKHWVIPLSALSFMVLTYLVVVGSSNAVNLTDGLDGLAIMPTVMVSSALGIF
                     AYLAGHLYFSKYLGIPYVAGAGELLVFCAAMAGAGLGFLWFNAYPAEVFMGDVGALAL
                     GAALGVIAVIVRQEIVLFIMGGVFVMETFSVAAQVLYFKYTKKKYGVGRRIFLMAPLH
                     HHYEQKGWKETQVVVRFWIISMVLVLIGLASLKIR"
     gene            complement(2308..3687)
                     /gene="murF"
                     /locus_tag="PKL42_04665"
     CDS             complement(2308..3687)
                     /gene="murF"
                     /locus_tag="PKL42_04665"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNU66401.1"
                     /translation="MMMLSEAAAALGASLIGADVPFTSVGTDSRYITRGQLFVALKGD
                     RFDGHDYVEQAKSLGASAVLVSDNKGVGPAIFVNDTCQALGELAKYWRKKFKIPVMAI
                     TGSNGKTTIKEMMIAILACHGGGVEKIHATVGNLNNHIGLPLTLLKLRATHQYSVLEM
                     GMSHLGEIAYLTDIATPDVAVISNAGTAHIGEVGSRHNIAKAKGEIFSGLASNGVAVI
                     NVDDDYAEYWKSLNASRKIVTFGLNADADVSATFEEKSGVCHVQLKTKRGDADFILNT
                     LGIHNVRNALAACAAAIALEIPIKEIAVGLSQFHAVKGRLQRKVGVNNAVVIDDTYNA
                     NPDSMKAAINVLESLSGKKILVLGDMGELGIDASQMHAEIGAYARAANIDTLYCLGEL
                     SKEAVNSFGVGAKHFTHVDLLINALLQEIKPGDSVLVKGSRFMKMERIVNAIVLDADN
                     KNLTKEEVH"
     gene            complement(3684..5156)
                     /locus_tag="PKL42_04670"
     CDS             complement(3684..5156)
                     /locus_tag="PKL42_04670"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNU66402.1"
                     /translation="MSILLQLDFPQQKYTSITSDSRMVQKGSLFLAYPGTQSDGRKYI
                     AQAIEAGAGAIVWERKQTETVDFSWDSAWRVKNVPCDDLKLHAGEIAAEFYDYPSKKV
                     TMIGVTGTNGKTSVSQWVAQCLTELDKKTAVIGTVGNGFYKHQTEAANTTPDAVLLQA
                     MLAKYVECKAEMVVMEVSSHGLEQGRVNAVEFDYAVFTNLSRDHLDYHITMEAYTKAK
                     QKLFEKRGLKASVINIDDIVGREIVESLQAQGRRCVTYGFMKADIQATNLVLHPDGLQ
                     MNVTVGSKDYLLSAAVLGRFNAYNLLAVLAVLIELKVNVQDAIAAIASIKSVQGRMQQ
                     IGGGNLPLVVIDYAHTPDALEKVLSTLKEHANSKLICVFGCGGNRDKGKRPLMGKVVS
                     NLADIVLITNDNPRNEDPATIIREITANMHGEYQVEMDRAIAIEKAIHLSGRGDVVLV
                     AGKGHENYQEIAGVKYPFDDAEVALMALSKYQASNMEKSA"
     gene            complement(5153..6895)
                     /locus_tag="PKL42_04675"
     CDS             complement(5153..6895)
                     /locus_tag="PKL42_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNU66403.1"
                     /translation="MALRTAPVIVKLPAWRRRLLLIAVLLGFVVLLGRGLYLQSFHKK
                     FLQAKGDARYSRTLVLPAHRGKITDRNGELLAISSPVESVWASPPDVEITSAQVQQVA
                     ALLGMKSSELSKKLVNTERGFVYLKRRLSPELAAKVMKVGVPGIYLQREYKRFYPAGE
                     VTAHLVGFTGVDDTGQEGFELAMNASLSGKAGSRRVIKDRSGHIVEDLEAVNVPQDGR
                     DLVLSIDRRIQYLVHRELAKAVELRKAKAGAAIILDAKSGEVLAMVNLPTYNPNNPVN
                     IKGKTRNRAITDMFEPGSTLKSVAVAAGLESGQYQPDTKIQTAPGHMSIGPATIHDSH
                     PHGILSVAEVIQKSSNVGASKIALSLDRLYLWNIFNQLGFGVKTNIGFPGEATGRVRD
                     YKTWRPIEQATMSYGHGISVTLLQLARAYTVFANDGELKPVSLLKLNESPVGIQVFSP
                     KTANQMKDMLEMVVQPGGTALKAQVVGYRVAGKTGTTHKLGDKGYEKNKYVGSFVGIA
                     PASNPRLIMAVMIDEPSIENKQYYGGTVAAPVFSAVMADALRMLAVPQDSPNTNIVIE
                     EDVTENVDDVSESI"
     gene            complement(6895..7212)
                     /gene="ftsL"
                     /locus_tag="PKL42_04680"
     CDS             complement(6895..7212)
                     /gene="ftsL"
                     /locus_tag="PKL42_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HNU66404.1"
                     /translation="MTRLNIILFIALILSGLGLVNAQHKARNLYFGLEQLNQAAKQYE
                     QEYGLLQLEQSTWAMHSRIEEVATQELQMQVPDAKRVQVVTLEKPITSAATHLNEPLK
                     GAP"
     gene            complement(7209..8249)
                     /gene="rsmH"
                     /locus_tag="PKL42_04685"
     CDS             complement(7209..8249)
                     /gene="rsmH"
                     /locus_tag="PKL42_04685"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNU66405.1"
                     /translation="MIKGVHNAPTQASLSLKKASVTPVGSLVENKEKSLQATNSHITV
                     LLDEAVEALAVKPDGVYVDGTYGRGGHSKKILAQLGSSGRLVAFDRDLSAIESGKAIQ
                     DSRFTMVHSHFSAMQAKLTQLGISKVDGILLDLGISSPQIDEASRGFSFRFDAPLDMR
                     MDQSRGQTVADYLAATTEQHLGEVIKNYGEERFAKQIARTIIAQRNDGHAITTTGQLA
                     KIVAGAVTRFEPGQNPATRTFQALRIFINQELEELSLTMPQCLALLAPQGRLAVISFH
                     SLEDRMVKQFIRNQENTDHLPANFPVRAADLPLPKLKSVGKAIKPSLAEIKANPRSRS
                     AVLRVAERTLET"
     gene            complement(8246..8692)
                     /gene="mraZ"
                     /locus_tag="PKL42_04690"
     CDS             complement(8246..8692)
                     /gene="mraZ"
                     /locus_tag="PKL42_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNU66406.1"
                     /translation="MFRGATHLSLDAKGRIAVPTKHREALQLQCAGDLVLTAHPHRCL
                     LLYPQPAWEPIQAKMMALSSFDKQSSALQRLLVGFAEDVALDSAGRMLVSPVLRDFAG
                     LDKEVMLVGQGSHFELWNMEAWRAQLAQVLQEEGFNMPAELEGFSL"
     gene            complement(9136..9503)
                     /gene="rnpB"
                     /locus_tag="PKL42_04695"
     ncRNA           complement(9136..9503)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKL42_04695"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            9776..10390
                     /locus_tag="PKL42_04700"
     CDS             9776..10390
                     /locus_tag="PKL42_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEPxxWA-CTERM sorting domain-containing protein"
                     /protein_id="HNU66407.1"
                     /translation="MKIAKLTAALLLSLGSTLSQAAVFTENFNGFTADQLNWNPAGAN
                     GWVVGPTANDTVDLIGQGGDFDLLPGNGRYVDLDGSTGSSGVLRNFVDLIAGTQYTLS
                     FWLAGNQRLNQGTESVNVHFGGVNQTFNLTSYLDPFNTFSLIFTPTATNRYSFGFTTN
                     SNDNVGALLDNVSVTAVPEPETYALMLAGLGLIGFSARRRKTQA"
     gene            10637..11251
                     /locus_tag="PKL42_04705"
     CDS             10637..11251
                     /locus_tag="PKL42_04705"
                     /inference="COORDINATES: protein motif:HMM:NF038126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxDxF family PEP-CTERM protein"
                     /protein_id="HNU66408.1"
                     /translation="MKNNIKQLALAAVIATGVSSINASAATLEILAATAGNTYEQNFG
                     FGATLGGPDALVYGTPEPTTHYSDFIEFVLTSPSSIIVFAKNLSSGFAFDVNPLDTVP
                     VIDLKDSLGNYILKNHDHAALNPAADGSYSYYYPNIYSDYIPLAAGKYTVNFNYGPKV
                     TSLTGGTYTGGITVAAVPEPETYAMMLAGLGLIGFSARRRKTQA"
     gene            complement(11434..11991)
                     /locus_tag="PKL42_04710"
     CDS             complement(11434..11991)
                     /locus_tag="PKL42_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNU66409.1"
                     /translation="MQIFKTKRAVTSVTGTSMLGVSFQRSLVSAIVLGFMSMTALAIT
                     PDPADQSGYKREFTLLDVDGNGKLSASEIQKDALFDGGGFGKADKNGNKSLSENEYAT
                     YKSAVQQKETKRVAKDSAITSKVKSKYLLEKNFKSFDVSVETKEGVVLLSGFVDNEST
                     KARAGQIAAGVKGVKAVKNGVVVKP"
BASE COUNT         3392 a         3142 c         2237 g         3424 t
ORIGIN      
        1 cattgcgcgg tatatcggca cgcctgttga atgcaaaacc gcctcaatca attgagcatc
       61 acgaccgatt aacaccactg cacgggcatt gctttgcact gcatctttaa gagggctaaa
      121 atcttgcccc ttaccatcac cacctgcgat taacactact ttttgtggta atccacctaa
      181 cgcagcgcaa gttgctccaa cattagtacc tttagaatcg tcgtaataat ctacatcatc
      241 gatgttcgca atccactcaa cacgatgtgg caatccttta aagttataaa gagtttggat
      301 aatcggagcg aaatcaatac caataccacg acacaacgca acagcagcaa gcgcattagc
      361 cgcgttgtgt aagccaaata atttaaggtc tcgcatatta atcaattctt tttcaccaaa
      421 gcagagccat gtctcgccat tggttagtcg cagcccatac tcaccctcat ctggctcact
      481 tagtccaaat gtcacttgtg ccagctttgg acgcgccatg agcatactcc acgcatcatc
      541 acggtttagc acttgcaatt ttgcattgta aaaaatacgt gccttggcaa tcgcgtagtc
      601 agacatggcc gcgtatctat ccatatgatc ttcagataaa ttgagcatcg tggctgcatc
      661 taggtatagc gtatcggttg tttcaagctg aaaactagat agctccaaca cataaacatc
      721 tggcacatcc atcattaagg cgtctaatac aggcaatcca atgttgccgg caacgattgt
      781 tttcagacct gcggctctgc acacttcacc cactaaggtc gtaacagttg ttttgccgtt
      841 ggcgccggta attgcaatga ctttggcgtt tctatttcga tactgcgcaa atagctctac
      901 gtcaccaacc acgcttactc cgcacgcaat cgctgcttta atttcaggct cgttaagcga
      961 tacacctggg cttaatacaa tcaaatctac actacgcaac aatgcggctg taaaggtacc
     1021 ggtaaacact tttagtgcag gaaactctgc ttttacctta tctaaatttg gtggattctc
     1081 acgtgaatca gcaacagaca aacgtgcacc ctggcactct aaccagcgca atgccgacag
     1141 gcctgtatca cccaagccta ccaccaacac gtttttgtct ttaagtacca tcataattta
     1201 tctaattttt aagctagcca gcccaattaa taccagtacc atgctgataa tccaaaaacg
     1261 caccacaact tgcgtttctt tccagccttt ttgctcatag tgatgatgaa gtggtgccat
     1321 taaaaatatt cgccgcccaa ctccatattt tttcttggta tatttaaagt agagcacttg
     1381 tgctgcaact gaaaaggttt ccatcacaaa cacgccaccc ataataaaca ggacgatttc
     1441 ctgacgaaca ataacggcaa tcacgccaag cgcagcaccc agtgccaacg cacctacatc
     1501 ccccataaaa acttccgcag gataggcgtt aaaccataga aaacctaaac cggctcccgc
     1561 catcgccgca caaaatacca acaactcgcc ggcgcctgct acgtaaggaa tgcctaaata
     1621 ttttgaaaag tatagatgtc ccgccaggta agcaaatatc cccaacgcac ttgacaccat
     1681 gactgtaggc ataatggcta aaccatctaa accatctgtt agattcaccg cattgctgct
     1741 acccactacc accaaataag tcagaaccat aaaactcaat gcactgagtg gaatcaccca
     1801 atgtttaaaa aatggcacaa tcaatgtggt ttccactggc gtttttgctg ttttatatag
     1861 aaatacagcc actgcaacac ctatcaagct ttgccagaaa tacttagctt ttgcagatag
     1921 ccctttagga tttttgtaaa ccaccttacg ataatcatcg acccaaccaa cagcaccaaa
     1981 tcctagcgtt gtaaacaaca cgacccagat aaagcggtta cttaaatcac cccaaagcag
     2041 ggttgaaatt gcaatagcaa ctaagataag cgccccaccc attgtaggtg tgccagattt
     2101 aattaaatgc gtctgaggtc catcatcccg aacggcatga cccactttat attccgttag
     2161 ctttcgtatc atgctggggc caaccatcag tgatatcgcc aatgacgtga gtgtggctaa
     2221 aaccgcacgt aatgtaatgt aatcaaatac cgaaaaccct cttatgtgct gtacttttgc
     2281 caaccattgc gccagatcta ataacattta gtgcacctct tccttcgtca gatttttatt
     2341 gtcagcatca agcactatcg cattcacaat gcgctccatt ttcataaagc gcgatccttt
     2401 caccaacaca gagtcgcccg gtttaatctc ttgcaacaag gcattaataa gtaaatcgac
     2461 atgggtaaaa tgctttgccc ctacaccgaa actattaacc gcctctttgc ttaattcacc
     2521 caagcaataa agcgtatcaa tattggcagc cctagcatat gcgcctattt cagcatgcat
     2581 ttgtgaggcg tctattccta actctcccat atctcccaat actaaaatct tcttacccga
     2641 taaactttcc agcacattaa tcgccgcctt cattgagtct gggttagcgt tataggtatc
     2701 atcaataaca accgcattat tcacaccaac cttacgctgc aacctgcctt tgactgcatg
     2761 aaactgactt aacccgaccg caatctcttt tattgggatt tccaaggcaa tagccgccgc
     2821 acaggccgca agcgcatttc gaacattgtg tataccgagc gtatttaaaa taaaatccgc
     2881 atcacccctt tttgttttga gctgaacatg gcaaacacca gacttttctt caaaggttgc
     2941 agaaacatct gcatcagcat tgagcccaaa agttactatt tttctacttg cattaagcga
     3001 cttccaatac tcagcgtagt catcatccac attaatgact gccacaccgt tacttgctaa
     3061 cccagaaaaa atttcacctt tagcttttgc aatattgtga cgagagccaa cctcaccaat
     3121 atgcgcagtt cctgcgttgc taatcaccgc cacatcaggt gttgcaatgt ctgttaagta
     3181 cgctatttcg cccaagtgac tcatccccat ttccagcact gagtattgat gagtagcacg
     3241 tagttttagt aaagtgagcg ggagcccgat atgattattc aaatttccga ccgtggcatg
     3301 tattttttca acaccacctc catggcaagc caatatggca atcatcattt ctttaatcgt
     3361 cgttttaccg ttactgccag taatcgccat cactggaatc ttaaattttt ttcgccaata
     3421 cttagctaac tcacctaatg cctggcaagt atcatttaca aatatcgctg ggccgacacc
     3481 cttattgtca ctgaccagaa ctgctgaagc gcctagactc ttagcctgct ccacataatc
     3541 atggccgtcg aaacggtccc cttttaacgc cacgaatagc tgtccacgtg tgatgtaacg
     3601 gctatcagta cccacacttg taaatggcac atcagcacca atcaagcttg cgcctaaagc
     3661 agccgcggct tctgaaagca tcatcatgca gacttctcca tatttgaagc ctgatatttt
     3721 gacaaggcca tcagcgccac ttccgcatcg tcaaatgggt acttcacacc tgcaatttct
     3781 tggtaatttt catgcccttt tccagccacc agcacgacat caccacggcc tgagagatga
     3841 attgcctttt cgatggcaat tgcgcgatcc atttcaacct gatattcgcc atgcatattc
     3901 gcagtgattt cacgaatgat tgttgccggg tcttcattac gtggattgtc gttggtaatt
     3961 aacacgatat ccgcaaggtt acttactact ttccccataa gcggacgctt tcctttgtct
     4021 cgattaccgc cgcaaccaaa aacacaaatc aattttgagt ttgcatgctc tttgagggta
     4081 cttaatacct tttccaatgc atctggcgtg tgtgcataat cgataactac taaaggtaaa
     4141 ttacccccgc caatttgctg catacgccct tgtaccgact taatcgacgc aatggcggct
     4201 atcgcatcct gcacattcac cttaagctct atgagaactg ccaacactgc aagcaagtta
     4261 tacgcattaa agcgccctaa gacggccgca cttagcaaat aatctttgct accaacagta
     4321 acgttcatct gcaatccgtc tggatgtaat actaagtttg ttgcttgaat atcagctttc
     4381 ataaatccat aagtgacaca tcgcctacct tgcgcctgaa gactttcgac gatttcccgc
     4441 ccgacgatat cgtctatatt gataacggat gccttcaagc cgcgcttttc aaaaagcttt
     4501 tgtttggctt tggtatatgc ttccattgtg atgtggtaat ctaaatgatc tcgactcaag
     4561 ttggtaaaca ccgcgtagtc aaactccaca gcattaactc gcccttgctc aagcccgtgt
     4621 gatgaaactt ccatcaccac catctcagcc ttacattcga catatttagc taacatggct
     4681 tgcaacaaaa ccgcatctgg tgtggtatta gccgcctccg tttgatgctt gtaaaagcca
     4741 ttaccaacgg tgccaatcac tgctgttttc ttgtctaatt ccgtcaagca ctgcgcaacc
     4801 cactgactaa cagaggtttt accgttcgta cctgtcacac caatcatcgt cactttctta
     4861 gagggataat cataaaattc agctgcaatc tctcccgcat gaagctttaa atcatcacac
     4921 ggtacgtttt ttactcgcca agctgaatcc caagagaaat ccacagtttc tgtttgcttt
     4981 ctctcccata caatcgcacc agcacccgct tcaatggctt gtgcaatgta ttttctgcca
     5041 tcactttgcg taccagggta agccaaaaac aatgatccct tttgcaccat tcgactgtcg
     5101 ctagtgatcg atgtgtattt ttgctgcgga aaatctaact gtaagaggat gctcatatac
     5161 tctcacttac gtcatcgacg ttttcagtaa catcttcttc aattacgata tttgtgttgg
     5221 gagaatcttg tggcactgcc agcattctta gcgcgtctgc catcacagca ctaaatactg
     5281 gggccgccac agtgccgcca taatattgct tattttctat actcggctca tcaatcataa
     5341 ccgccatgat taagcgagga ttagaagcgg gtgcaattcc tacgaatgat cctacatatt
     5401 tatttttttc atagccttta tcacccaatt tatgtgtcgt acctgttttt cctgccacac
     5461 gataacccac tacttgcgcc tttagcgccg tcccgcctgg ctggactacc atttcaagca
     5521 tatctttcat ttgatttgct gtttttgggc taaatacctg aatacctaca ggcgactcat
     5581 ttagctttaa taatgaaact ggctttaact caccatcgtt ggcaaatact gtataagctc
     5641 ttgcgagctg caataacgtg acactaattc catggccata tgacatggtt gcctgttcaa
     5701 taggccgcca agttttatag tcgcgcaccc taccagtcgc ttccccagga aagccaatat
     5761 tcgttttcac gccaaagcca agttggttaa atatattcca cagatataaa cggtccaaag
     5821 atagtgcaat ttttgatgcg ccaacgtttg aagatttttg aattacttct gctaccgata
     5881 aaatcccgtg cggatgcgag tcatgtattg tggctggccc tatactcata tgaccaggag
     5941 ctgtctgaat ctttgtatcg ggctgatact gacctgattc tagacctgct gctacggcca
     6001 ccgacttcaa tgtagagcct ggctcaaaca tatcagtaat cgcgcgatta cgcgttttac
     6061 ctttgatgtt aacggggtta ttggggttgt aagtcggaag gttgaccatc gccagcacct
     6121 cgccggattt ggcatctaat atgatggcgg cgcctgcttt tgccttgcgt aactccaccg
     6181 ccttagctag ctcacgatga acaagatact gaattctacg atcaatcgac aacactaaat
     6241 cgcgcccatc ctgcggaaca ttgacagcct ctaagtcctc aacaatatga ccagacctat
     6301 ctttaatcac gcgacggctt ccagccttgc ctgataaaga ggcattcatt gccaactcaa
     6361 agccttcctg cccggtatca tcaactcctg tgaaaccaac cagatgcgcc gtcacctctc
     6421 ctgcagggta aaaacgtttg tactcgcgct gcaggtatat gccaggaaca ccaactttca
     6481 tgacttttgc agccaactct ggcgacaatc tacgttttaa gtatacgaac ccacgctcag
     6541 tattcacaag ctttttactc agctctgatg atttcatgcc caaaagtgct gccacctgtt
     6601 gcacttgtgc agaagtaatt tcaacatctg gcggacttgc ccaaacagac tctacaggcg
     6661 aacttatcgc cagcaactcg ccattcctat cagtgatttt tccacgatgt gcaggcaaaa
     6721 ccagcgtgcg gctataacgt gcatctcctt ttgcttgcag aaacttctta tgaaaactct
     6781 gcaaatacaa tcctcggcct aacaacacta caaagcccag caacacagcg atcaacaaca
     6841 atctgcgtcg ccatgctggc aatttcacaa taaccggtgc tgtgcgcaaa gccattatgg
     6901 tgcccccttc aatggctcat tcagatgagt tgctgccgat gtaatgggct tttccaaggt
     6961 gaccacttgc acacgctttg catccggtac ttgcatctgc aattcttgcg ttgcaacctc
     7021 ctctatacgc gaatgcatcg cccacgtgct ctgctctaat tgcaaaagtc cgtactcttg
     7081 ctcatattgc ttggcagctt gatttagctg ttcaagccca aaatagagat tgcgtgcttt
     7141 atgttgcgca ttcaccaacc ctaaacccga taaaatcagc gctataaata atatgatatt
     7201 taagcgcgtc atgtttccag cgttctttct gccacacgca gcaccgcact gcgtgagcgg
     7261 gggttggctt ttatttctgc caaactgggc ttgatggcct tgcccacgct cttgagtttt
     7321 ggtagcggca agtcagcagc cctcactgga aaattcgcag gtaaatggtc ggtattttcc
     7381 tgattgcgaa taaactgctt caccatacga tcttcaagcg aatgaaagct aatcacagct
     7441 aaacgcccct gcggtgccag caatgccaaa cattggggca tggttagcga taactcctca
     7501 agctcttgat tgatgaaaat ccgtagagct tgaaaggtgc gcgttgcagg gttctgccct
     7561 ggctcaaacc gcgtgactgc ccctgccacg atcttggcaa gttgccctgt agtggtgatt
     7621 gcgtgcccgt cattgcgctg cgcaataatc gtccttgcaa tctgcttagc aaaccgttct
     7681 tcaccataat tctttataac ctcccctaaa tgttgctctg ttgtagcggc aagataatct
     7741 gccaccgttt gtccacgact ttgatccata cgcatatcca gcggcgcatc aaacctaaaa
     7801 ctaaacccac gactcgcctc atcaatctgt ggagatgaaa ttcccaagtc cagcaaaatt
     7861 ccatcaactt tactaatgcc caattgagtc agcttggctt gcatcgcaga aaaatggcta
     7921 tgcaccatgg taaaacgcga atcttgtata gccttgccag attcaatcgc actcaagtca
     7981 cgatcaaacg caaccaacct accactgcta cccaactgtg ctaatatttt ttttgaatgt
     8041 ccgccacgcc catacgtacc atccacataa accccatcgg gctttacagc cagcgcctcg
     8101 acggcctcat ctagcaaaac agtaatatgg gagtttgttg cttgtagact tttttcttta
     8161 ttctcaacca gcgaaccaac gggtgttact gatgcttttt ttagagacaa cgatgcttgc
     8221 gtgggcgcgt tgtgcacgcc cttaatcaca gtgaaaagcc ctctaattct gccggcatat
     8281 taaatccttc ctcttgcaac acctgtgcaa gttgagcacg ccatgcctcc atgttccaca
     8341 attcgaaatg actgccttgc ccgaccaaca tcacctcttt atctaacccg gcaaaatctc
     8401 gcaacacggg tgacaccaac atgcggccag cactatccag cgcaacatct tctgcaaaac
     8461 ccaccaataa acgctgtaat gcactcgact gcttgtcaaa tgaagacaaa gccatcatct
     8521 tggcttgaat tggctcccac gcgggttgtg gatacaacaa caagcaacga tgcggatgcg
     8581 cggtcaacac caaatcacca gcacactgaa gctgtagcgc ctctctatgc ttggtcggca
     8641 cagcgattct gcctttagca tccaaactta aatgtgtagc cccgcgaaac atgattgatg
     8701 aattaaccta aaataattac taacaaaacc aatatggata aaccaatatt aaaaggtgag
     8761 aaatacctaa ccacccttag catcagttag atatttccca caaaaaccca ctttttccca
     8821 ctaaattgaa ctatagataa aaaaaaagag caatgcaagc tctttttgtg taaattttct
     8881 ttatatctca atgacttagc gcatttttct cataaaaata atatcgttat aaattaagga
     8941 catacaaaat taagtgaatg tagattatta ggatagtgtt gaaagcgatt gaattgtaat
     9001 tagcgagatt tatagggtga gaatgaaaat atattggcgg cctttttata ccagaaaatc
     9061 tttctgagtt tgactttaag accacaaagg tactcgctat gcacttaagc caccaaagcg
     9121 gcaagtaaag agagaaaacg aagctggccg ataagccggg ttttgtagac cttattacta
     9181 aggctgacag tcattcatct aggcgcataa ttactcatgc gctcaagcaa cctacccgct
     9241 ggcaacgcga gccacgtcaa tgccagccta tttggtcttg ctgcagatgg aggttaccgc
     9301 gtttcacccg tttcgacgca agctcgtaac tttcgttact cgcaggtcga accgactcgt
     9361 ctctgtggcc ctattccgca tcttataatc tgccagcttt cgccagcaaa catttcaatg
     9421 tacggccgtt agccgtcatc ccgctctatg cagcccggac tttcctcccc ctattgctag
     9481 gcggcgactg tctggccagc ttcaagtgca ttttaacaca ctggacagct aaacaaatcc
     9541 ttaagccttt tgcaaaattt tcacttaaaa tcaagagttc agttaatatg aaactcatat
     9601 tcatgcaaat tgtttaatcc aaactgtaaa cttaagtaaa taaacgatat ttttcaattc
     9661 cattcacccg aatgggctat tgcaccagat gtagtcatta actagcatga gtacatcaac
     9721 atgttgtgtt ggtaatttac aaagaattag tacattctca agataggtgg caattatgaa
     9781 aatcgcaaaa cttaccgcag cactactcct tagcttaggt tcaacactat cacaagcagc
     9841 cgtgtttact gaaaacttta atggctttac agctgatcaa ttaaattgga atccagctgg
     9901 tgcaaatgga tgggtagttg gccctacagc caacgacacg gtcgacctaa tcggtcaagg
     9961 aggtgacttt gacctgttac caggcaatgg ccgctatgtt gacttagatg gatctacggg
    10021 ttcttcagga gtcttaagaa actttgtgga tttaattgca ggcacccaat acacgctatc
    10081 attttggtta gctgggaatc agcggttaaa tcaaggtacg gagtcagtca acgtgcattt
    10141 tggtggtgtt aaccaaacgt ttaacttaac aagttacctt gacccgttca atacatttag
    10201 cttgattttc accccaacag caaccaatag atatagtttt ggattcacca ccaacagtaa
    10261 cgacaatgtc ggcgcactgc ttgacaacgt ttcagtgact gctgttcctg aaccagaaac
    10321 ctacgcatta atgcttgctg gacttggttt gattggtttc tcagcccgcc gtcgcaaaac
    10381 acaagcctag ataaaataaa ttagtttaga taaatttgtt gctttcaaag caagtcttaa
    10441 aagcccacgt aattgtgggc tttttcttgt ttggcagaga aatcaattaa aactcaccca
    10501 cccaatagcc cgttcgggta attgccacat ctttaaacaa caaatatcat cgagcgactt
    10561 agataatatt tggattatct acaaagcata tacgcctagg taaccaactt aacaaaactt
    10621 tagggaaaac ttaaaaatga agaacaatat taaacaatta gcgttagctg ctgtaattgc
    10681 aactggcgta tcaagcatca atgcaagcgc agcaacactc gaaattcttg cagctacagc
    10741 aggcaatacg tacgaacaaa actttggttt tggcgctaca ctaggcggcc ctgacgcgct
    10801 tgtttatggc actcctgaac ctactaccca ttactcagac tttatcgaat tcgtactaac
    10861 ctcaccttca agcattatag tctttgcaaa aaatctatca tcaggttttg catttgatgt
    10921 taatccttta gatacagtgc cagtgattga ccttaaagac tctttaggca attatatttt
    10981 gaaaaatcat gatcatgccg ccctaaatcc tgcagcagac ggtagttaca gctattatta
    11041 tccaaatatc tactctgact atataccttt agcggcaggt aaatacacgg tgaatttcaa
    11101 ttacggtcct aaagttacaa gccttacagg tggaacctat actggtggca ttactgttgc
    11161 tgccgtgcct gaaccagaaa cttatgccat gatgcttgcc ggccttggct taattggttt
    11221 ttcagcacgt cgtcgtaaaa cgcaggctta agttttatca ttaattgcta gcacagcgca
    11281 aacaaacccc accgatgtgt ggggtttgtt tttttatgct tgcacaactc ttaaactttt
    11341 tacttagtca ctcatcgcct gacaaagttt ttagcgttaa aaaattaatc tttcaaaata
    11401 gataactttg tattcacggg ttaatgcatt tagttagggc ttaaccacaa ctccgttttt
    11461 tactgctttc acgcccttca ctccagctgc aatttggccg gcgcgcgctt tagtggattc
    11521 gttatcaaca aatccgctaa gtaaaacaac gccttctttg gtttctacac tgacatcaaa
    11581 gcttttaaag tttttttcaa gcaggtattt tgattttact tttgaagtaa tcgcgctgtc
    11641 ttttgccacg cgcttggttt ctttttgctg cacggctgat ttataagttg cgtattcatt
    11701 ttcacttaaa cttttattgc cgtttttgtc tgccttgcca aatccgccgc catcaaaaag
    11761 cgcatctttt tgaatttctg acgcactaag cttaccgttg ccatcaacat cgagtaatgt
    11821 aaactcgcgt ttgtagccgc tttggtctgc cgggtctggc gtaatggcca acgccgtcat
    11881 gctcataaac cctagcacaa tcgccgaaac taaacttcgt tgaaaactta cgcctaacat
    11941 gcttgttcct gtaacgcttg taaccgcccg ttttgtttta aaaatttgca tctagtcacc
    12001 tccgtaaaaa taaacaatac gccatttaaa aaccaacgca aaactacttt tagtgtataa
    12061 gtgcgcctaa aaataatgcg cctaaaatcc tcctaaaatc agtactctgg caaccacttt
    12121 agcatgctta cgttctttca atgcatttca tttggacgct ctaacattag tgtgacttta
    12181 tagcggtgca attca
//
LOCUS       DAOQSI010000049        11969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_4469_length_11969_cov_6.332634, whole genome shotgun sequence.
ACCESSION   DAOQSI010000049 DAOQSI010000000
VERSION     DAOQSI010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 11969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11969
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4469_length_11969_cov_6.332634"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..1001
                     /gene="folD"
                     /locus_tag="PKL42_04715"
     CDS             141..1001
                     /gene="folD"
                     /locus_tag="PKL42_04715"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HNU66410.1"
                     /translation="MTAQIIDGKAIAESLLNSIKTRIQQRLQANKRAPGLAVILVGAD
                     PASAIYVRNKRLACEKVGIVSKAYDLPSSTTEAELLALIAELNEDDTIDGILVQSPLP
                     PQIKDEHVIEHISPYKDVDGFHPYNIGRLAVRQPTLRSCTPFGVIKMLEATNISLMGL
                     DAVVVGVSNHVGRPMALELLLAGCTVTSCHRHTKDLAKQVMQADLVVAAAGKHGLIQG
                     EWIKPGAIVIDIGINRLENGSIAGDVDFAAAKQRAGYITPVPGGVGPMTVATLMENTL
                     LALELKEKAQ"
     gene            1125..1547
                     /gene="dksA"
                     /locus_tag="PKL42_04720"
     CDS             1125..1547
                     /gene="dksA"
                     /locus_tag="PKL42_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HNU66411.1"
                     /translation="MADVISKQFTPYQPKPDEQYMSKAQLNHFRKILNDWKSELSHDL
                     DNTIHTMQDEVTMFADPNDRASQESDMTLELRNRDRERKLIKKIDETLRNIDEGDYGY
                     CEGCGIEIGLKRMEARPTATLCIDCKTLDEMREKQVAK"
     gene            complement(1883..4012)
                     /gene="pnp"
                     /locus_tag="PKL42_04725"
     CDS             complement(1883..4012)
                     /gene="pnp"
                     /locus_tag="PKL42_04725"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNU66412.1"
                     /translation="MFNKVTKSIQYGAHTLTLETGEISRQADAAVLVSYGDTVVLVAV
                     TSKSEVKAGQDFFPLTVDYMEKTYAGGKIPGGFFKREGRPSEKETLTSRLIDRPLRPL
                     FPEAFYNEVQVVATVLSSDPEIDADIPAIIGASAAMSLSGIPFFGPLGAARVGYINDE
                     YVLNPTKAQLEETALDLVVAATETAVMMVESEAKELSEEIMLGAVVYGHEQMQAVIKM
                     ISELAAEAGKDAWDWVAPEPDTALIEKVAALAAADINAAFQIKAKGARSTKLSEITNR
                     VLAELITEETSTTEANKIKNEIFNLEAKTVRSQILNGEPRIDGRDTRTVRPISIRTGV
                     LPRTHGSALFTRGETQALVVATLGTGRDEQVIDALQGEFKDRFMLHYNMPPYATGETG
                     RVGTPKRREIGHGRLAKRALVAALPEKEDFDYTMRVVSEITESNGSSSMASVCGGCLA
                     LMDAGVPMKNHVAGIAMGLIKEGNRVAVLTDILGDEDHLGDMDFKVAGTEDGITALQM
                     DIKITEITAQIMQVALAQAKEGRAHILGIMKQAVSGANAEMSQFAPRIITMKINPEKI
                     RDVIGKGGAVIRALTEETGTSIDIDDDGTVKIACTSSEQGAEAQRRIAEITAEVEIGN
                     VYEGTVLKLLDFGAIVSLIPGKDGLLHISQIAHQRVNAVGDFLKEGDVVKVKVVEADE
                     KGRVRLSMKALIDPPAPEAKAESEADE"
     gene            complement(4209..4478)
                     /gene="rpsO"
                     /locus_tag="PKL42_04730"
     CDS             complement(4209..4478)
                     /gene="rpsO"
                     /locus_tag="PKL42_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNU66413.1"
                     /translation="MATTAADRAKIVSEFQTAPKDTGSPEVQVALLTSRIAYLTEHFK
                     SNKKDNHSRRGLLALVSQRRRLLDYLKSKDVTRYQALIGRLGLRK"
     gene            complement(4628..5542)
                     /gene="truB"
                     /locus_tag="PKL42_04735"
     CDS             complement(4628..5542)
                     /gene="truB"
                     /locus_tag="PKL42_04735"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNU66414.1"
                     /translation="MQYKRPKKNINGILLLDKPHGFSSNQALQKVKWLLSAAKAGHTG
                     TLDPLATGLLPLCFGEATKFAHYLTDADKTYVATLKLGVTTNTGDAEGAVLSTQPVNA
                     TQAQFEAACRLFVGEISQTPPMYSALKFEGKAMYEYARAGVEIARQPRAVMIHQIIVH
                     SFAGDVAEITVTCSKGTYIRTLAEDIGNALGCGAHLIGLRRTATANYHIHQSVTLSQF
                     EATPAEARIALLMPADSAVQHLAAITLDADSTFYLQQGQAVWKSDVVPTGLLRLYDEQ
                     QQFLGLGEQQSDGKIAPKRLIVNKPISG"
     gene            complement(5707..6063)
                     /gene="rbfA"
                     /locus_tag="PKL42_04740"
     CDS             complement(5707..6063)
                     /gene="rbfA"
                     /locus_tag="PKL42_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNU66415.1"
                     /translation="MAKDFSRSHRVAEQMQRELADLLQFEVKDPRVGMVTITAVEVTG
                     DMAHAKIFYSAAKASESMQQGLEKSAGFLRTQLAKRMLLRTVPQLHFVYDSSIDNGMM
                     MSKLIDEAIASNTHKT"
     gene            complement(6192..8858)
                     /gene="infB"
                     /locus_tag="PKL42_04745"
     CDS             complement(6192..8858)
                     /gene="infB"
                     /locus_tag="PKL42_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNU66416.1"
                     /translation="MAQITVEQFAGELKLPTALLLEQLKSAGVNKSNANDLMSETDKA
                     ALLEHLRKEHGTLAPKNKITLTRKSSTEIKKTDNTGKARTIQVEVRKKRVIEQRDELI
                     APIELTPEVPVSEPVVEEKVIVEQVPEIVEAVESAEVSIQPEAVSEPVVEAEPIVAKT
                     ISRKDLLGADEIALREQEAKRHSKLAAMQAEDKRKKEELAQKRLEEEAKRLAEAEAAK
                     AKVAKLSEGTLHRPVAKEGVAKPAAKDAKKGKGNNKDWTDAENKKRGIKTRGDTTLGK
                     AGWRAPKGKNRHHDDDAQHAFNAPTEAIVYEVLVPETITVAELAHKMAVKSAEVIKTL
                     MGMGMMVTINQVLDQETAIIVVEEMGHKASAAAPNDPETFIEETEHAEAILESRPPVV
                     TVMGHVDHGKTSLLDYIRRSRVASGEAGGITQHIGAYHVETPRGMVTFLDTPGHEAFT
                     AMRARGAKATDVVILVVSADDGVMPQTIEAIHHAKAAGVPLVVAINKIDKPGAEPERV
                     KMELVAQEVVPEDFGGEVMFREVSAKTGQGIDELLEAVLLQAEILELQAPRNTPAKGL
                     VIEGRLDKGRGSVATILVTSGTLKRGDMILAGNTFGKVRAMLDETGKDIQEAGPSMPV
                     EIQGLADVPSAGEEVIVLNDERKAREIANFRQGKFRDVKLAKQQAAKLENMFDQMGDG
                     AAQQTLGLIIKSDVQGSYEALATSLQKLSTAEVKVNIIHTGVGAISESDVNLAAASKG
                     VLIGFNVRADTGARKLIETLGIDVRYYNIIYEAVDEIKAALGGMLAPEQKESMIGTVE
                     IREVFRISKVGAIAGCYVQDGLIKRNSLVRVLRNNVIVHTGELDSLKRFKDDVKEVKN
                     NFECGLSLKNFNDIEVGDILEVYEIVEVARTL"
     gene            complement(8960..10426)
                     /gene="nusA"
                     /locus_tag="PKL42_04750"
     CDS             complement(8960..10426)
                     /gene="nusA"
                     /locus_tag="PKL42_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNU66417.1"
                     /translation="MSREILLLVDALAHEKNVSKEVIFTALELALASATKKKHHDDAD
                     IRVSIDRETGDYETFRRWQYVEYDLLENSNYQFDEESEHAKGRQIGDYYEEPLENIEF
                     GRIGAQAAKQVILQKVREAEREQILEDFLARDEKLVTGQIKRMEKGNAIIEVGRIEAL
                     LPREYMIPKENLRVGDRVRAYLSRVERGGRGPQLILSRISPEFLVRLFELEVPEIEEG
                     LLEIRAAARDPGLRSKIAVKTNDQRLDPVGTCVGMRGSRVQAVTGELAGERVDIVLWS
                     MEPAQFVINAMSPAEVSSIVVDEDAHSMDVVVDEEQLALAIGRNGQNVRLASELSGWT
                     LNILTVDQAAQKNQDEFAGVTQLFMQKLDVDEEVAEILVQEGFSTLEEIAYVPLAEMN
                     EIEAFDEDTIEELRKRARAALLTEAIAKEEKVEEAAEDLLQMEGMDDKTAHLLASKGV
                     ATMDDLAELSVDELVELTKMDSERAKMLIMTARAPWFK"
     gene            complement(10475..10960)
                     /gene="rimP"
                     /locus_tag="PKL42_04755"
     CDS             complement(10475..10960)
                     /gene="rimP"
                     /locus_tag="PKL42_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNU66418.1"
                     /translation="MQDLQALVEKTVVQLGFELVDFEMSNRGKLLRVFIDKLNPKNTK
                     DSVTIDDCVLVSNQLGNVLTVEHEIDYDRLEVSSAGMDRVLKKEKDFVRFVGERASIK
                     LRVGMQDLSANATATTLPRKTFLGVINGVEDGHVIVEFEGVLYKMALSNIDKARLSPV
                     F"
     gene            complement(11256..11369)
                     /gene="rrf"
                     /locus_tag="PKL42_04760"
     rRNA            complement(11256..11369)
                     /gene="rrf"
                     /locus_tag="PKL42_04760"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(11655..>11969)
                     /locus_tag="PKL42_04765"
     rRNA            complement(11655..>11969)
                     /locus_tag="PKL42_04765"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         3119 a         2993 c         2302 g         3555 t
ORIGIN      
        1 tcccaaagca ggcgcgctac caggctacgc tacacgccga agaggcagaa atatactgga
       61 aaccgagcga taggtcaatt cgaatatgtt aaaatgctgc attattttta acaattgata
      121 aaaattcacc cccaaaactc atgacagctc aaataattga tggcaaagcc attgccgaat
      181 cgctattaaa ctcaatcaaa acacgcattc agcagcgcct tcaggcgaat aaacgtgcgc
      241 ccggcttggc cgtcattctt gtgggcgccg atcctgcatc ggccatttat gttcgcaaca
      301 agcgccttgc ctgtgaaaaa gtaggcattg tttctaaagc ttacgactta ccgtcaagca
      361 ctacagaagc tgaactgctg gcgttgattg ccgaattaaa tgaagacgac acgattgacg
      421 gtattctcgt gcagtcaccg ctaccgccac aaattaagga tgagcatgtg attgaacaca
      481 ttagccctta taaagatgtg gatggctttc acccatacaa tattggcagg ctggcggtcc
      541 gccaaccaac gctacgttct tgcacgcctt ttggggtgat taaaatgctt gaagctacca
      601 atatttcgct gatgggttta gatgctgtgg tggttggtgt ttcaaatcat gtgggtcggc
      661 caatggcgct ggaattgtta ttggcgggtt gtaccgttac aagctgccat cgccacacta
      721 aagatttggc aaagcaagtg atgcaggcag atttagtggt tgcggcggca ggcaagcatg
      781 gattgattca aggagaatgg attaaacctg gtgcgattgt gattgatatt ggtattaacc
      841 gtttagaaaa tggcagtatt gctggtgatg tggattttgc agcggctaag caacgtgccg
      901 gctatattac ccccgtacct ggtggcgtgg ggccaatgac cgtggctacc cttatggaaa
      961 acactttact cgcattagaa ttgaaagaaa aagcacaata atcaaccagc aaatcttact
     1021 aataattcaa tcaattacac gattttttgc atcaaaactt ttttcggtgt acactcacgc
     1081 gcttaatttt atttaagtca cttttttagt ctttaaggca aaacatggca gacgttatca
     1141 gtaaacaatt tacgccctat cagccaaaac ctgatgagca atacatgagc aaagcgcagt
     1201 tgaatcattt tcgtaagatt ttaaatgatt ggaagtcaga actaagccac gatttagaca
     1261 atacgataca caccatgcaa gatgaggtga cgatgtttgc tgacccaaac gaccgcgcca
     1321 gccaagaatc tgacatgaca ttagaactac gcaaccgtga ccgtgagcgt aaactcatca
     1381 aaaaaattga tgaaacatta cgcaatattg atgaaggtga ctatggctat tgtgaaggtt
     1441 gcggcatcga aattgggtta aagcgtatgg aggcaaggcc cacggcaacg ctttgcattg
     1501 attgcaaaac gctcgacgaa atgcgtgaaa agcaggttgc taaatagcca acagctttca
     1561 gagaagccta acctcaaggt taggcccgaa ctaaaagcaa gttgcaaaat tattttgctt
     1621 aatttaaagc ccgatttttt cgggcttttt taatttatgt tgtaagtatt ttagcgaagt
     1681 atttcgcttt taaaatcaca ttaaatcacg ctgaaatctt gccgagaaaa aaataatgcc
     1741 ctacgagccc ttatttacaa ggctcgtagg gcatcgaaaa aggttgtgcg aattttattc
     1801 gcttttttta aaggtttttt cgttcccatt tccccaaaaa ttaagcccgc taaagcgggc
     1861 ttaattttta cctcatgaca gattattcat ccgcttcaga ttctgctttc gcctctggtg
     1921 caggtgggtc aatcaatgct ttcatgctta aacgcacacg gcctttttca tcggcttcta
     1981 ctactttcac tttcacaaca tcgccttctt ttaagaagtc acccaccgca ttcacgcgtt
     2041 gatgtgcaat ttgtgaaata tgcaacaagc catctttacc tggaattagc gacacaatcg
     2101 ctccaaaatc aagtaattta agcacagtac cttcataaac attaccgatt tcaacttctg
     2161 ctgtaatttc agcaattcga cgttgcgctt cagcaccttg ctctgaactt gtacaagcga
     2221 tttttaccgt gccgtcatca tcaatatcaa tgctggtgcc agtttcttct gttaatgcac
     2281 gaattaccgc gccgccttta ccaatcacat cacgaatttt ttcaggattg attttcatgg
     2341 tgataatgcg tggggcaaat tgtgacatct cagcatttgc gccgcttacc gcttgcttca
     2401 taatgcccag gatatgcgca cggccttctt tagcttgtgc taaagccact tgcatgattt
     2461 gtgcagtaat ttctgtaatt ttgatgtcca tttgtaacgc agtaatgccg tcttcggtac
     2521 cggccacttt aaagtccatg tcccctaggt gatcttcatc gcctaaaatg tcggtcaata
     2581 cagcgacgcg gttgccttct ttaatcagac ccattgcaat accggctacg tgatttttca
     2641 tcggcacgcc tgcgtccatc aacgctaaac agccaccgca aacagaggcc attgagcttg
     2701 aaccgtttga ttcagtgatt tcagacacaa cacgcatggt gtaatcaaag tcttcttttt
     2761 ctggcaatgc agccactaac gcacgttttg ctaaacggcc atgaccgatt tcacgacgtt
     2821 ttggcgtgcc cacacggccc gtttcacctg tggcatacgg aggcatgttg taatgcaaca
     2881 taaaacgatc tttgaattca ccttgcaatg catcaatcac ttgctcatca cggccagtac
     2941 caagtgtagc aaccactaat gcttgcgttt caccgcgtgt gaacaatgca gagccgtgcg
     3001 tacgtggcaa cacgcctgta cgaatgctga ttgggcgcac tgtgcgtgtg tcacggccat
     3061 caatacgcgg ctcaccattt aaaatttggc tacgtactgt tttagcttct aaattaaaga
     3121 tttcattttt aattttgttg gcttcagtgg ttgaggtttc ttctgtgatt aactcggcta
     3181 atacgcgatt tgtgatttct gacagcttcg ttgaacgcgc gccttttgct ttgatttgga
     3241 atgctgcgtt aatatccgct gctgccaaag ccgctacttt ttcaatcaat gcagtgtctg
     3301 gctcaggagc cacccaatcc catgcgtctt tacctgcttc agcagcgagt tcgctaatca
     3361 ttttaatgac tgcttgcatt tgctcgtgac catataccac cgcgcccagc atgatttctt
     3421 ctgaaagctc tttcgcttct gattccacca tcatcacagc agtttcggtt gcggcgacga
     3481 ctaagtctaa cgctgtttct tctaattggg ctttggttgg gtttagtaca tattcatcat
     3541 taatataacc aacgcgtgcc gcacctaatg ggccaaagaa tggaatgcct gacaatgaca
     3601 tggcagcaga agcgccgata atggctggaa tatctgcatc aatttcaggg tcagatgaca
     3661 atacagtcgc taccacttgc acttcgttat aaaacgcttc tggaaataag ggacgcaatg
     3721 gacggtcaat taaacggctc gtcaatgttt ctttttctga aggacggcct tcacgtttga
     3781 agaagccccc tggaatttta ccgcctgcat aggttttttc catgtaatcc acagtgagtg
     3841 ggaaaaaatc ttggcctgct tttacttctg atttgctggt cacagccact aacacaacgg
     3901 tatcaccata actcaccaac actgctgcat cggcttgacg tgagatttca cctgtttcaa
     3961 gcgtaagcgt atgcgccccg tattgaatgc ttttcgttac tttattaaac atgtcttttc
     4021 cttttcaatt caataaactg ttattcacta cccaataaca gctgttttac tgctttaatt
     4081 ttaatttagt gatatttttg gctgatattg ccgccatgaa aaaagccgac gcatatcacc
     4141 atgcagtcgg cttttcttac atcataaaag tgcgctatat ctaaacaagc aatgcacttt
     4201 tacaacaatt acttacgtaa acctaaacgc ccaatcagtg cttgataacg agtcacatct
     4261 ttgcttttta aataatctag caaacgacga cgttggctta ccaatgccaa caaaccacgg
     4321 cgtgagtgat tgtctttttt gtttgattta aagtgctcgg ttaaataagc aatacggctg
     4381 gttaaaagtg ccacttgcac ttctgggctg cctgtatctt ttggtgctgt ttgaaattcg
     4441 cttacgattt tagcgcgatc tgctgcagta gttgccataa cttaattcct aataatttag
     4501 atgttttgcg tcaatgtaaa agcagcctaa aagttaaacc gctaaaacat tcatttgcat
     4561 gaaccgagca ttgtaacgat aaccgaatga tttatcaaat atttattgcc ttaaacgcaa
     4621 aaaacaatca gccagatatc ggtttattga caataagccg tttgggcgca atttttccat
     4681 cgctttgttg ttcaccaagc cctaaaaatt gctgctgttc gtcataaagc cgcaacaatc
     4741 ctgtaggcac cacgtcactt ttccataccg cttggccttg ctgcaaataa aatgtactgt
     4801 cggcatctaa cgtgatggcc gctagatgtt gcactgcact atcggctggc atgagcagtg
     4861 caatacgcgc ttctgccggc gttgcttcaa actgggacaa ggtcaccgat tgatggatgt
     4921 gatagtttgc ggtagcggtt ctgcgtaaac caattaaatg tgcgccgcaa cctagcgcgt
     4981 tgccaatatc ttctgccaat gtgcgaatat acgtgccttt gctgcaagtt acagtgattt
     5041 ccgccacatc ccccgcaaat gaatgaacga tgatttggtg aatcatcacg gcacgcggct
     5101 gacgcgcaat ttctacacca gctctagcat attcgtacat tgccttacct tcaaatttga
     5161 gcgcggagta catcggcggc gtttgactaa tttcgcccac aaacaggcga cacgctgctt
     5221 caaactgcgc ttgtgtcgca ttgactggtt gcgttgataa aaccgcacct tccgcgtcac
     5281 cggtattggt ggtgacaccc aacttcaatg tggccacata agttttatcg gcatctgtca
     5341 ggtaatgcgc aaatttagtt gcctcgccaa aacaaagcgg gagcagtcca gttgccaacg
     5401 gatccagcgt gccggtatgg ccagcctttg ccgcagacaa cagccatttt actttttgca
     5461 gggcttggtt agatgaaaaa ccatgcggtt tatctaacag caggattccg ttaatatttt
     5521 ttttggggcg tttatactgc acagggattt ttacaattga tgtgtgtttg cgaattttca
     5581 gaggttaaat ttacaatcaa aaaattgcat tttctccact aaaaatgtta cgagaaaaaa
     5641 tttctgccct gcgagccttt atttataagg cttccagggc atcgaaaaag gttgatgttt
     5701 taaaacctat gttttatgcg tatttgaagc gatcgcttca tcaattaact tagacatcat
     5761 catgccgttg tcaattgaac tgtcatagac aaaatgcaac tgcggtacgg tgcgaagcaa
     5821 cattcttttt gccaattgcg tacgcaaaaa tcctgcggat ttttctagcc cttgttgcat
     5881 gctttcgctt gcttttgcag cgctataaaa gattttagcg tgcgccatat cgcccgtgac
     5941 ttcaaccgca gtaatggtca ccatgccaac ccgcgggtct ttcacttcaa actgcagcaa
     6001 gtcagccaac tcacgctgca tctgttctgc aacgcggtgg ctgcgtgaga aatcttttgc
     6061 catatttatc ctattaaatg caaagtgtta agctaggctg ggcgtgaaac aaaaaattga
     6121 gcgcggcata cattgagtat gtaagcaaaa gttttctgtt ttgcaacaac gcatcgcgac
     6181 aaagtttgca tttatagcgt acgtgccact tctacgattt catacacttc taaaatatcg
     6241 ccaacttcaa tatcattaaa gtttttcagc gataatccgc actcaaagtt gtttttcact
     6301 tctttcacat catctttaaa gcgtttgagt gaatcgagct cgcctgtatg aacaatcaca
     6361 ttattgcgca gcactcgcac cagtgaatta cgtttaatca gcccgtcttg tacataacaa
     6421 ccagcaattg cgcccacttt agaaatacgg aatacttcgc gaatttccac agtaccaatc
     6481 atgctttctt tttgctccgg tgccaacatg ccgcccagcg ctgctttaat ctcatcaact
     6541 gcttcgtaaa taatattgta gtagcgtacg tcaattccta aagtttctat caacttacgc
     6601 gcacctgtat cagcacgtac gttaaagccg atcaacacac cttttgaagc tgccgccaag
     6661 ttcacatcag attcgcttat cgcgccaacg ccagtatgaa taatattaac cttcacttca
     6721 gcggtcgata atttttgtaa gctggtcgct aatgcttcat aagaaccttg tacgtcagac
     6781 ttgataatta accctaaggt ttgctgtgca gcaccatctc ccatttggtc aaacatattt
     6841 tcaagtttgg ccgcctgctg tttcgccaat ttaacatcgc ggaatttacc ttggcggaaa
     6901 ttagcaattt cacgcgcttt acgctcgtca ttaagcacaa tcacttcttc accagcactt
     6961 ggcacatcag ctaaaccttg aatttcaacc ggcatggaag gacccgcctc ttgaatatct
     7021 ttacctgttt catccagcat ggcgcgcact ttaccaaaag tgttgccagc caaaatcatg
     7081 tcaccgcgtt taagcgtgcc agatgtcacc aaaattgttg ccacagaacc acggccttta
     7141 tccagacggc cttcaatcac caaacctttt gctggtgtgt ttcttggcgc ttgcaactct
     7201 agaatttcag cctgtaacag taccgcttct aacagttcat caataccctg accagttttt
     7261 gctgaaactt ctctaaacat cacttcgcca ccaaagtctt ctggcacaac ttcttgcgcg
     7321 actaattcca tttttacacg ctctggctca gcgccaggct tatcaatttt attgatggcc
     7381 acaacaagtg gcacacctgc cgctttagca tgatgaatcg cttcaattgt ttgtggcatc
     7441 acaccatcat cagctgaaac cactaaaatc accacgtccg tcgctttggc accacgagca
     7501 cgcatggccg taaacgcttc atggcccggc gtatccaaaa aggttaccat gccgcgtggc
     7561 gtttctacgt ggtacgcccc aatgtgttgc gtaataccgc cagcctcgcc tgaggctaca
     7621 cggctacggc gaatataatc gagtaatgaa gtcttaccgt ggtctacgtg acccatcacc
     7681 gtgactactg gtgggcgcga ctcaagaatc gcctcagcgt gttctgtttc ttcaataaat
     7741 gtttcagggt cattcggcgc cgcagcagag gctttatgcc ccatttcttc caccacaata
     7801 atagcggtct cttggtctaa cacttggttg atggtcacca tcatgcccat gcccatgagt
     7861 gttttaatca cctcagcaga ctttactgcc attttgtgcg ccagctctgc aaccgtaatt
     7921 gtctcgggta caagcacttc gtaaacaata gcctctgtag gtgcattaaa ggcgtgttgc
     7981 gcatcatcat cgtgatgtct gtttttgcct tttggtgcgc gccagcctgc cttacctaag
     8041 gttgtatcac ctcgcgtttt aatgccacgt tttttattct cagcatccgt ccaatcttta
     8101 ttattgcctt ttcctttttt ggcatctttt gctgctggtt ttgctacgcc ttctttagcc
     8161 actggtctat gcaaagtccc ctctgatagt ttcgctacct tagcttttgc tgcctcggct
     8221 tcggccagtc gcttagcttc ttcttctaat cttttttgcg caagttcttc ttttttacgc
     8281 ttatcttctg cttgcatggc ggccaacttg gagtgtcgct tggcttcctg ctctctgagt
     8341 gcaatctcat ccgcacccag taaatctttt ctgctaatcg ttttagcgac aattggctca
     8401 gcctctacaa ctggttcact tacagcttca ggctgtatag acacttcagc actttccaca
     8461 gcttcaacga tctcaggcac ttgctcgact atcacttttt cctcaaccac aggctcactc
     8521 acaggaactt ctggtgttaa ttcaataggt gcaatcaatt catcgcgctg ctcaatcaca
     8581 cgtttttttc gcacttccac ctgaatcgta cgagccttac cggtgttgtc cgttttttta
     8641 atttctgtgc tagattttcg tgtcagtgtg attttatttt taggcgcaag cgtaccatgc
     8701 tctttacgta agtgttccag taaagctgct ttatccgttt cgctcattaa gtcattggca
     8761 tttgatttat tgaccccagc acttttgagc tgctctaata acaatgctgt aggcaacttt
     8821 aattcgccag caaattgttc tacggttatt tgtgccatct tgttactcca gtattctttc
     8881 taagcttaat tgcttcgcct taattagttc tattacatca ttgacttggc gccatacaag
     8941 caccaagctg tttctgcaat cacttaaacc atggcgcgcg tgctgtcatg atgagcattt
     9001 tggcgcgttc gctatccatt ttagtcagct ccaccaattc atcgactgat agctctgcta
     9061 aatcatccat ggttgccaca cccttagagg ccaacaaatg agctgtttta tcatccatcc
     9121 cctccatttg tagtagatcc tcagcggctt cttctacttt ttcttcctta gcaattgcct
     9181 cggttaacaa agcagcgcgt gctcgcttac gaagctcttc aatcgtatcc tcgtcaaaag
     9241 cttcaatttc attcatttcc gctaatggaa cataagcgat ttcttctaaa gtactaaaac
     9301 cttcctgcac cagaatttct gctacttctt catcaacatc cagtttctgc atgaacaact
     9361 gcgtcactcc agcaaactcg tcttgatttt tctgcgccgc ctgatcaaca gttaaaatat
     9421 ttaaagtcca acccgatagt tcagaagcaa gacgaacatt ttgaccatta cgtccaattg
     9481 ccaaagccaa ctgctcttcg tccaccacaa catccatgct atgcgcatct tcatctacaa
     9541 caatgctcga cacttcagca ggtgacatcg cattaatcac aaactgtgca ggctccatgc
     9601 tccataacac aatatccacg cgctctcctg ccaactcacc tgtgaccgct tgtacgcgtg
     9661 agccgcgcat accaacacat gtacccactg ggtctaaacg ctgatcattt gttttaactg
     9721 caatctttga acgcagtccc gggtcacgtg ctgcagcacg tatttccaac aatccctctt
     9781 ctatttcagg cacttctaat tcaaataagc gcactaaaaa ttctggtgaa attctagata
     9841 atattaactg cggtccgcgg ccaccacgct ctacacgcga taaataagca cgcacacgat
     9901 cacccacacg caaattttct ttcggaatca tatattcgcg cggtagcaac gcctcaatac
     9961 gacccacttc gataatcgca ttgcccttct ccatacgctt aatttggcct gtgactaatt
    10021 tttcatcacg cgctaaaaag tcctctaaaa tttgctcacg ttctgcttcg cggacttttt
    10081 gcaaaataac ttgctttgca gcctgtgcgc caatacgccc aaattcaatg ttttctagtg
    10141 gctcttcata atagtcacca atttgacgac ctttagcatg ctcactctct tcgtcaaact
    10201 gataatttga gttttccagt aaatcatatt ccacatattg ccagcgccta aaggtttcat
    10261 aatcgcccgt ttcgcgatca atagatacgc gaatatccgc atcatcatga tgttttttct
    10321 tagtggcaga tgctaaagcc aattccagtg cagtaaaaat cacctcttta ctcacgtttt
    10381 tttcatgcgc caacgcatca actaataata aaatttcacg actcattgca atctccgact
    10441 taaaatttac tttttaataa cgattactta ttatctaaaa tactgggctt aaccgtgctt
    10501 tatcaatgtt gctgagcgcc attttataca acacgccttc aaattcaact atcacatgtc
    10561 cgtcttcaac accatttata acacctaaaa aagtcttgcg tggcaatgtg gttgccgtag
    10621 cgttcgcgct taaatcttgc atacctacac gcagcttaat actggcgcgc tcacctacaa
    10681 accgcacaaa atctttttcc ttcttcagca ctcgatccat cccagcagat gaaacttcca
    10741 atctgtcgta atcaatctca tgctcaaccg tcaatacatt acccaactga ttactcacca
    10801 gcacacagtc atcaatagtc acactgtctt tagtgttttt aggattcagc ttatcaataa
    10861 acacgcgcaa cagcttaccg cggttagaca tttcaaagtc aaccaactca aagcctagct
    10921 gcaccactgt tttttcaacc aacgcctgca aatcttgcat tacacaacac ccttaaacta
    10981 cgtatcgagc agaaacaaaa aatgggcttg aagcccatct cattttttcg cacattatac
    11041 caatttttta caagaattgg aatcactgac gcaactgtat tcttcgcaat gtaacgccgc
    11101 catatcgcat ctagctcgaa ttccgttcgt agcagtaaat gttttcgaac ctctgctcta
    11161 tacttgggct tcataaccta aaaggctaaa aagcaaaaag cccaacattg ctgttgggct
    11221 ttttgttcac tactttgcag tagtatcaat aaatagcttg gcagtgacct actttcgcat
    11281 gcaaaaagca tactatcatt ggcgctggtt cgtttcacgg ccctgttcga gatgggaagg
    11341 ggtggttcca aaccgctatg gccgccaagc ataactggta tcactgatgt ggttgacttt
    11401 ttagtcagct tcatcagcag aaatgcttag tatcaataac gatacatgtt cacctgcttg
    11461 ctgtgctacg agccacgtct aacttttcac gttgcgcatt tcacagccat caggcgtctg
    11521 taacaaattg gaagaagtaa agtgtttgat gtcgttgaca tcaattaaat ggggttgatt
    11581 acattatctt ttctttagaa taacgcttat ctcgattgtc acctttcagt aacttggtca
    11641 agttttaagg ttataggatc aagcctcacg agcaattagt aacggtcagc ttaatgcatt
    11701 actgcacttc cacacccgac ctatcaacgt cctggtctcg aacgactctt taggggggtt
    11761 aaacccccag ggaaatctca tctcaaggcg agtttcacgc ttagatgctt tcagcgttta
    11821 tctcttccgc acttagctac ccagctatac cattggcatg ataactggtc caccagcggt
    11881 gcgtccactc cggtcctctc gtactaggag caggtccctt caaatttcca gcgcccacgg
    11941 cagataggga ccaaactgtc tcacgacgt
//
LOCUS       DAOQSI010000050        11878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_4529_length_11878_cov_4.832276, whole genome shotgun sequence.
ACCESSION   DAOQSI010000050 DAOQSI010000000
VERSION     DAOQSI010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 11878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11878
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4529_length_11878_cov_4.832276"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PKL42_04770"
     CDS             complement(<1..200)
                     /locus_tag="PKL42_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166425.1"
                     /note="macrophage inducible 5; Mig-5; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNU66419.1"
                     /translation="MYKHPLLNRDKMSPQEALDILIEGNHRFTNSLTANKDLMRLVSI
                     TKDRQHPFVSVLSCSDSRAPVE"
     gene            complement(296..1681)
                     /locus_tag="PKL42_04775"
     CDS             complement(296..1681)
                     /locus_tag="PKL42_04775"
                     /inference="COORDINATES: protein motif:HMM:NF017591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF839 domain-containing protein"
                     /protein_id="HNU66420.1"
                     /translation="MQKWLKFIPFLLSLMGCSSILPHDKSSAAMIHPTAAGWQVRPLI
                     SVGQPAENGYRMTGIPDGMGAFDNDDGTFTLFVSHEIAADKGEVVTHGAKGAFVSSWR
                     IDVETLQPLSGQDLIQKVHLWDAQSRQHQQVKGAAFSRFCSADLAKRGTFYDATTGAG
                     YQDYLFLNGEEDKTSGRAFAHTLAGESYELADLGKIQWENAVANPTLSKQTLVIGMDD
                     NHDKGLVLVYIGQKRKAGNPVELAGLVGGQLYAVKVDAKRFSLVPLAQVDTMNIGTFR
                     NHALAQGATLFARPEDGAWNTLNNHQFYFATTDKIGGNPQLLSLQFDDIQQPALGGTF
                     SVVVNGKDFGAQMFDNITVDGDGHVLLEEDPGNDARLAAIWRYMPQSKKIEKIFESNP
                     DFFKVGEKKFLTINEEHSGIIEITHLVKKASWFAQNKRYFLGNTQVHPQGEADRAIYM
                     KYGQIWMIAGQ"
     gene            complement(1791..3230)
                     /locus_tag="PKL42_04780"
     CDS             complement(1791..3230)
                     /locus_tag="PKL42_04780"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNU66421.1"
                     /translation="MNGNKLRGVIAAVAGVMMFNAMPAMADSTDDILNALIAKGVLTE
                     EEGALLQKGRTGEKEAAAKKKESSISTSFKDGIVFESGDKQHKMSINGRAQLDFRSFS
                     NEVTSNQAETFDIRRAYLSAKGSFYDTYDFELTENSGDLLYAYFNVRWWEQAQFKLGQ
                     FKQPFSMEELGSSRFLNFTERSFVTQLVPAVDRGAQIHGTPFKGVTYALGVFNGVGIN
                     RGQNANDTSIDADGKSVTGRATVNIAELMDQKDAVYHLGAAYSDTSDNHDVNAFAIRT
                     EGRGSNIFQSTAPGAVGATNKGYDLKRTGLEGAVAYGPVKFQTEWIKAEFEPDGNNRL
                     VAGAPVGQEKDITAWYAAVNWMITGESYADVYKGGLFGGRMKPKNNFDPKAGKWGAGA
                     WEIGARYSKLDASDFSLGAETADVFNGTNENGKFNEADAWTLGLKWIPSPNVRFLANY
                     VTTDMDCVKGFVCTEDKEKAVNLRAQIDF"
     gene            complement(3369..4226)
                     /locus_tag="PKL42_04785"
     CDS             complement(3369..4226)
                     /locus_tag="PKL42_04785"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNU66422.1"
                     /translation="MNTALLAIAGSVAMHVAWNLLVRQQGTKTRLLWWALATHCMILA
                     PWGVYALVTQAHWGPQLTMALLVSSLTNVAYFVALDRAYHRAPVALVYPIVRSSPILI
                     AIWSMLFFGETFGIGAWLAMLLSVAGLILLASTAWRNDANAALPWALAAALATSAYSL
                     SDKAAAKFLPTLSSVLGFISVGYALSFIAITVLLRREHGRWCPERRPPLLIMLFAGLC
                     IGLAYVLVIHAMRNMPAAVVVAFTNAGIVIAGALSIFMFRERQHWKQRLIGMVVVMLG
                     LIVLAIVQI"
     gene            complement(4223..4975)
                     /gene="phnP"
                     /locus_tag="PKL42_04790"
     CDS             complement(4223..4975)
                     /gene="phnP"
                     /locus_tag="PKL42_04790"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HNU66423.1"
                     /translation="MQLTFVGTGSTTAAPLYGCDCEVCTTARTNPTFKRNPASVLVEA
                     GGSRILIDAGLHNLGERFAAGTFEAFLITHFHADHVQGLLPIRWGVGNKIAVYGPPDT
                     EGCADLYKNPGLLDFCTVHKFESFSIGEITITPVPLIHSKPTLGYCLAWRDTRIAYLT
                     DTVGLPPATADFLRQWQPTQLILDCSFPPRLDMPKGHNDVTRAIESIALVQPEHAWIT
                     HIGHELDVWLNQYMDALPTNISIARDNMVIGR"
     gene            complement(4966..5541)
                     /gene="phnN"
                     /locus_tag="PKL42_04795"
     CDS             complement(4966..5541)
                     /gene="phnN"
                     /locus_tag="PKL42_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism
                     protein/1,5-bisphosphokinase (PRPP-forming) PhnN"
                     /protein_id="HNU66424.1"
                     /translation="MAEGKLFYVIGPSGSGKDSLMSYARKRLASKSGIVFAHRYITRP
                     ANAGGENHIALSEEEFAARVHSSLFAMYWESHGCRYGIGSEVNIWLAKGYNVVMNGSR
                     EHLHEAQKSHPKLIPVWIEVAPEVLAKRLRARGRENETEISERLKRASVYQATPDGIV
                     IRNDGELQEAGDKLVQVIAPLTTSRVGEKCN"
     gene            complement(5534..6688)
                     /locus_tag="PKL42_04800"
     CDS             complement(5534..6688)
                     /locus_tag="PKL42_04800"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HNU66425.1"
                     /translation="MSKIYLTHANVVLENETLHDASVLIENGMIAAINPEQVSDAEEI
                     NLHGQTLIPGMIDLHCDALEKEVEPRPNVHFPLDFACAQADKRNAAAGVTTVFHALSF
                     ANHELGVRNNAFAAEIARAVHAWQPHALVDNRVHCRYEVTDPTAPEVLLDLMQKGDMH
                     LFSVMDHSPGQGQFKDITAFRDYLARTYKKTDAELDALLAEKAAQSEGAMQRIETLIE
                     AARGFGIRVASHDDDTPEKVQTVHALGVGISEFPINLAAAQAAKANGMFTIFGAPNIL
                     RGKSQSGSMKALDAVKENVADCLCADYAPAALIVAIFRLPELAGISLADAIRLVTINP
                     ARAVGLDDRGVIAVGKRADLVAVAHINGLVQASSVWTNGRLAYQARFDHG"
     gene            complement(6685..7425)
                     /gene="phnL"
                     /locus_tag="PKL42_04805"
     CDS             complement(6685..7425)
                     /gene="phnL"
                     /locus_tag="PKL42_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003118408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HNU66426.1"
                     /translation="MQTPILKINGFAKHFTLHEQGKQIPSSHNVNLEVYAGELTALIG
                     PTGAGKSSVLKGIYRTYLPSAGEILYRDRHGELVDLAQADEYRMLQLRHEEIGFVTQF
                     LFCLPRQSTLDVVAHPLVVRGECRESARNKAAEMLAALNLPERLWSISPATFSGGEKQ
                     RVNLARGLVAKPRLLLLDEPTASLDPVTAERVIGIIEGIKQEGVAMLAIFHHPELTHR
                     LGDRVVELTPPSAFLKTQAIDKQEKVYA"
     gene            complement(7435..8265)
                     /locus_tag="PKL42_04810"
     CDS             complement(7435..8265)
                     /locus_tag="PKL42_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNU66427.1"
                     /translation="MRKILEVRNLSKIYGKGCPRCIESTGPEADTNMCPHCGSVVAIH
                     DVSFDLHEGEILGLMGESGSGKSTVVKNLYFDQTPTSGSARFIEEDATHEMFNLNAVQ
                     QRRLRNQRFGMVYQNPHLGLNFGISSGGNIAERLLMSDVSHYGEIRERAKELLSRTEV
                     LLSRMDESPAAFSGGMQQRVQIAKALATNPPLLYLDEVTTGLDLSVQARILDLILEIQ
                     QERNTAMIVVTHDLGVIRLLTGRALVMKYGRVIESGLTDQILEDPQHAYTQRLVASAL
                     "
     gene            complement(8258..9127)
                     /locus_tag="PKL42_04815"
     CDS             complement(8258..9127)
                     /locus_tag="PKL42_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003118411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HNU66428.1"
                     /translation="MSVTYNFGFMDEHAKKEVRRSILKAISIPGYQVPYSSREMPMGR
                     GFGTGGLQITLSLIGADETLKVIDQGSDDSVNAVNLRQFVELTCPGVNTTTHLNAATL
                     IQSRHRIPEVPLTPNQTLVLQVPYPDPLVVVEPSEDKRKVMHGEADYARLLVKLYEDI
                     VKFDEITISHRYPTRINGHYVIDPSPIPRWDVPKLNDSPALILFGAGREKKIYAVPPH
                     TKAEPLAFDDVPFRVEGFNDAHGNRRPCERCGATDSFLDEYINEDGSKRYQCSDSDYC
                     DTVLAETKEAANA"
     gene            complement(9124..10248)
                     /locus_tag="PKL42_04820"
     CDS             complement(9124..10248)
                     /locus_tag="PKL42_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HNU66429.1"
                     /translation="MGYVAIKGGGQAIRGAEDVLETLRASEGAAGVPLSIDTIENQLR
                     LLTSRIVSEGGLYHPKLAALAIKQSQGDTLEAAFALRAYRSTMPRLMETQAQDTSKMR
                     LIRRISSAFKDIPGGQMLGPTYDYALRLMRLDLTDESPEKFRDIAHQFLEQTPEPNLP
                     DTFPKVVEALREEGLLPQAEVSEALAFDITREPLIFPVPRSAALATMARAETGSLLAL
                     AYSNMRGYGDVHPTIAELRVGYLPVMLPHPITGELTEAGEVLMTECEVVAMYEDDSNQ
                     GKPTFTLGYGACFGHNEVKAISMAILDRALQKGMKEGASNPSSDPEFVLLHVDGVDSM
                     GFASHYKMPHYVTFQSDMDRLRTTQQKHEDSKKTKMGDAQ"
     gene            complement(10252..10815)
                     /gene="phnH"
                     /locus_tag="PKL42_04825"
     CDS             complement(10252..10815)
                     /gene="phnH"
                     /locus_tag="PKL42_04825"
                     /inference="COORDINATES: protein motif:HMM:NF017645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HNU66430.1"
                     /translation="MSNLATAIPTIWQPHTQQHAYRQLMQAFANPGQIETLDEDALLL
                     TLATLLDGATSIADVHGLISELDLARLETVNAATETAHFVLAEGGQVPAFTPSLGTLE
                     SPEGGATLLLTVTAFNDGTPYTLSGPGVDGARNVTLQGLNSAWLDSRESWNAAFPMGV
                     DIVLLAGKQVMALPRTTKISTTSIKGA"
     gene            complement(10808..11284)
                     /gene="phnG"
                     /locus_tag="PKL42_04830"
     CDS             complement(10808..11284)
                     /gene="phnG"
                     /locus_tag="PKL42_04830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HNU66431.1"
                     /translation="MLNNQSQRAQLPRLLMALPEDEVIGAAASFAEQYTIEDIKLPQA
                     GLGLMQLKDSALQEGYFLGEIPLATAHVVLTDAQQNRTEGAAQIMHSEATLARAIAIM
                     DAVVSAKLNGHEGVTQLLARGLTVLTEQQSSRKKMLARTRVDFSLLSSAEEEDADE"
     gene            complement(11379..>11878)
                     /gene="phnE"
                     /locus_tag="PKL42_04835"
     CDS             complement(11379..>11878)
                     /gene="phnE"
                     /locus_tag="PKL42_04835"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HNU66432.1"
                     /translation="RNYTYNKFTYTLARGTISLLRSMPELIVALFLVLAYGFGPIAGV
                     LALGLHAAGFLGKFYAEDIENADKKPQEALEAIGAGKLKTLWYGVMPQVVPQYIAYTL
                     YILDRNVRMATVIGLVGAGGIGQELKGRFDMFQYDHVATILIAIFIVVFLLDQFSARL
                     RAKLI"
BASE COUNT         3088 a         3124 c         2611 g         3055 t
ORIGIN      
        1 atttcaaccg gcgcgcgcga atcgctgcaa ctaagcactg aaacaaaagg atgttgccgg
       61 tctttggtaa tgctcaccaa acgcatcaag tctttattgg ccgttaagct gttggtaaag
      121 cgatgattgc cttcaatcaa aatatctaag gcttcttgcg gcgacatttt gtcacggttg
      181 agtagtgggt gcttgtacat gtagatttct cgctaaagta gtgttcagat tatacctgag
      241 ctaacgcttt ttttaagaat cgaaactaga actagagcag ccaagactaa gacatttatt
      301 gcccagctat catccaaatt tgcccatact tcatgtagat tgcccgatct gcctcacctt
      361 gtggatgcac ttgcgtatta cctaaaaaat agcgtttatt ttgcgcaaac caactcgctt
      421 tcttcactaa atgcgtaatt tcaatgatgc ccgaatgttc ttcattaatg gttaaaaact
      481 ttttttcacc caccttaaaa aaatcaggat ttgattcaaa tattttttca atttttttgc
      541 tttgcggcat atagcgccaa atggcggcca atcgcgcatc atttccgggg tcttcttcta
      601 acagtacgtg accatcgcca tctaccgtga tgttatcaaa catttgcgca ccaaaatctt
      661 tgccattcac caccaccgaa aaggtgccgc ctagtgcagg ttgttgaata tcatcaaact
      721 gcaatgacaa caattgagga ttaccgccaa ttttgtcggt agtggcaaag taaaactgat
      781 gattgtttag cgtattccaa gccccatctt caggacgagc aaataaagtt gcaccttgtg
      841 cgagcgcatg atttcgaaaa gtaccgatgt tcattgtatc cacctgcgct agcggtacta
      901 aactaaaacg ttttgcatct actttcactg cataaagctg accacctacc aagcctgcta
      961 actccactgg gttacctgct ttgcgttttt gcccaatata gaccaacacc aagcctttat
     1021 catggttgtc atccatgccg attaccaacg tttgtttact taacgtaggg ttagccactg
     1081 cattttccca ctgaatttta cctaaatccg ccaactcgta actttcacct gccagcgtgt
     1141 gtgcaaaagc gcggcctgat gttttatctt cctcaccatt taaaaataaa taatcttgat
     1201 aacctgcccc tgtagttgca tcgtagaatg tgccacgttt ggctaaatca gccgaacaaa
     1261 aacgactaaa cgcagcacct tttacctgtt gatgttgcct tgattgcgca tcccaaagat
     1321 gtactttttg aatcaggtct tggcctgata aaggctggag ggtttctaca tcaatacgcc
     1381 agcttgaaac aaaagcgcct ttagccccat gcgtgactac ctcacctttg tctgcagcaa
     1441 tttcatgact tacaaatagg gtaaaggtac catcgtcatt atcaaatgcc cccatgccat
     1501 ctggaatacc tgtcatacga tagccatttt cagcaggctg acctacagaa atgagcggac
     1561 gtacttgcca gcctgccgcc gttggatgaa tcattgccgc agacgattta tcgtgaggta
     1621 atattgatga acaacccatc agagataaca agaagggaat gaatttaagc catttttgca
     1681 tttcaacgtc ctactaaatc aaaatttctt aaaaaaaagc ccgcttgcgc gggctaaagg
     1741 gcttgtagcc taagccacaa ggagaacctt attgactttt actataagaa ttagaagtca
     1801 atttgtgcac gcaagttaac ggctttttct ttgtcttcag tacatacaaa gccttttacg
     1861 caatccatgt cagtggtcac gtaattagct aagaagcgca cattcgggct aggaatccat
     1921 tttaaaccaa gcgtccaagc atccgcttca ttgaatttac cattttcatt agtaccattg
     1981 aatacatcag cggtttcagc acccaaacta aaatcagagg catctagctt gctgtatcgc
     2041 gcaccaattt cccatgcacc cgcaccccat ttaccggctt tagggtcaaa attatttttt
     2101 ggtttcatgc ggccaccaaa caaaccacct ttgtagacat ctgcatagct ttcaccagta
     2161 atcatccagt ttacagcggc ataccaagca gtaatgtctt tttcttgacc aactggagcg
     2221 cctgcaacca gcctattgtt gccatcaggt tcaaattccg ccttaatcca ttctgtttga
     2281 aatttaacag ggccataagc aactgcacct tccaagcctg tgcgttttaa atcataaccc
     2341 ttgttggtag caccgacagc accaggtgca gtagattgga atatattaga accacgacct
     2401 tcagtacgaa ttgcaaatgc attgacatca tgattgtcac tggtgtcaga atatgccgca
     2461 cctaagtgat acaccgcatc tttttgatcc attagctcag caatattgac tgtcgcacgg
     2521 cctgtgactg atttaccgtc ggcatctata ctagtgtcat tcgcattttg acccctattg
     2581 atacccacac cattaaacac acctaatgca taggtcacac ctttaaatgg tgtaccgtga
     2641 atttgtgcac ctcgatctac tgcaggcact agctgtgtca caaaagaacg ttcagtaaag
     2701 ttaagaaagc gtgagctgcc taactcttcc atgctgaatg gctgtttaaa ctgacctaat
     2761 ttaaactgag cctgctccca ccagcgcaca ttaaaatatg cgtataacaa atcacctgaa
     2821 ttttcagtca attcaaaatc ataagtgtca taaaatgaac ctttggcact taggtaggca
     2881 cgacgaatat caaaggtttc tgcctgatta ctcgtgacct cgttgctaaa tgaacggaaa
     2941 tctaactgtg cacggccgtt gatagacatt ttgtgttgtt tgtctccaga ctcaaacaca
     3001 ataccatctt tgaacgacgt gctaattgaa ctttcttttt tcttggcagc agcttctttt
     3061 tcaccagtac ggcctttttg taacaacgca ccctcttctt cagtcagcac cccttttgca
     3121 atcagcgcat ttaaaatatc atcggtgctg tccgccatgg caggcatggc attaaacatc
     3181 attacaccgg ctacagcggc aataacgccg cgtaatttat taccattcat tgaattactc
     3241 ccttttgaaa aattaaatat aaactgaact acgtataagc tttaagacga gtgcaattat
     3301 gaattttgaa tatgacatct ttgtgacacg tgactatttt tacaacgcag tgttgcgtaa
     3361 aaacaacgtc aaatttgaac aattgccaac acaattaacc ccaacatcac aacaaccata
     3421 cctattaatc gctgcttcca atgctgacgt tcgcgaaaca taaatatcga cagcgcacct
     3481 gcaatcacaa tacccgcatt agtaaatgcc accacgactg ccgccggcat gttgcgcatc
     3541 gcgtgtatca ccagtacata ggcaaggcca atgcagagcc cggcaaacag catgattagt
     3601 aacggcggtc tacgctcagg gcaccagcgg ccatgctctc gacgtaaaag tacggtaatc
     3661 gcaataaaag aaagggcgta accgacactg ataaaaccga gcacactaga aagcgtaggc
     3721 aaaaatttcg cagccgcttt atcagaaagc gagtaggcac tggtcgccag tgccgcagcc
     3781 aacgcccaag gtaacgcggc attcgcatca ttgcgccagg cggtagatgc tagcaatata
     3841 agcccagcaa cgctcaacag catggccagc caagcgccaa ttccaaaagt ctcgccaaaa
     3901 aacaacatgc tccagatggc aatcaaaatg ggtgaactac gcacaattgg gtaaaccagt
     3961 gccactggcg cacggtggta ggcgcggtct agcgcaacaa agtaggcaac attggtaagg
     4021 ctagatacca acaaagccat tgtaagctgt ggcccccaat gcgcctgcgt taccaatgca
     4081 tacactcccc acggggcgag aatcatgcaa tgggtcgcca gcgcccacca tagcagtcta
     4141 gttttagtgc cctgctgacg tacaagtaaa ttccatgcca cgtgcatcgc caccgaacca
     4201 gcaatggcta agagggcggt gttcatctgc caatgaccat attatcgcgc gcgatagaaa
     4261 tattcgtggg tagtgcatcc atatattggt ttaaccatac gtctagctca tgccctatgt
     4321 gagtaatcca agcatgttca ggctgcacta atgcaatact ttctattgca cgcgtaacat
     4381 cattatgccc ttttggcata tccaatcggg gtggaaagga gcaatcaagg ataagctgcg
     4441 tgggctgcca ttgcctcaaa aaatccgctg tagcaggtgg caaccctacc gtatctgtga
     4501 ggtaagcaat acgtgtgtcg cgccatgcca agcaataacc cagcgtaggt ttagagtgta
     4561 taagcgggac tggcgtaatc gtgatttcgc cgatgctgaa tgattcaaat ttatgcaccg
     4621 tgcaaaaatc cagcaatcca ggatttttgt ataagtctgc gcagccttcg gtgtctggcg
     4681 ggccatagac ggcaatctta ttacccacac cccagcgaat tgggagcaaa ccttgtacat
     4741 ggtcagcgtg aaaatgtgta atcaaaaagg cctcaaaagt gcctgcagca aaacgctcac
     4801 ctaaattgtg tagcccagca tctatcaata ttcgcgagcc gccagcctcg actaatacag
     4861 aggctgggtt acgtttaaac gttggattag tgcgcgcagt ggtgcaaact tcacagtcac
     4921 atccataaag cggtgctgcg gtggtagagc ctgtgccaac aaaagttaat tgcatttttc
     4981 gcctacccta gaggtagtca gtggtgcaat cacttgaacc aacttgtccc ctgcttcttg
     5041 caactcacca tcattacgaa tgacgatacc gtctggtgtc gcttgatata ctgatgcgcg
     5101 cttgaggcgt tcagaaattt ccgtttcatt ttcacgaccc ctcgcacgca atcgttttgc
     5161 caatacttct ggcgcaacct cgatccatac tggaattaat tttggatggc ttttttgagc
     5221 ttcatgcaaa tgttcacgcg atccgttcat caccacgtta taacctttag ccaaccaaat
     5281 gttcacttca ctgccaatac catagcggca accgtgactt tcccaataca tggcaaacaa
     5341 gctggaatgc acacgagcgg caaactcttc ttcacttaat gcaatatgat tttcgccacc
     5401 agcattagcc gggcgcgtaa tataacgatg cgcaaatacg atgcccgatt tgcttgccaa
     5461 tcttttgcgc gcatagctca tcaaactatc tttgccactg ccagaagggc caatcacata
     5521 aaaaagctta ccttcagcca tgatcaaacc tcgcctgata agcaagtctg ccattcgtcc
     5581 aaacactgga ggcctgcacc aaaccattga tatgcgccac cgcaactaaa tcagcacgtt
     5641 tacccacagc aatcacgcct ctgtcatcca aacctactgc acgtgctggg ttgatggtga
     5701 ccaaacggat agcatctgcc agcgaaatgc ctgccaattc aggcaggcga aaaatcgcaa
     5761 cgattaaagc cgctggggcg taatctgcgc acaggcagtc ggccacgttt tctttcaccg
     5821 cgtccagcgc cttcatcgaa ccagactggc ttttgccgcg taaaatgtta ggagcaccga
     5881 atatggtaaa catgccattg gccttggcgg cttgtgctgc ggccaagtta atagggaact
     5941 ctgaaatccc cacacccagc gcgtgtacgg tttgcacttt ttccggcgtg tcgtcatcat
     6001 gactcgccac acggatgcca aaaccgcgtg cggcctcaat caaggtttca atgcgctgca
     6061 tggcgccttc tgactgcgcg gctttttctg ccagcaaggc atcgagttct gcatccgttt
     6121 ttttgtaggt acgggctaaa taatcgcgaa aagcggtaat gtctttaaac tgcccttgcc
     6181 caggcgagtg atccatcact gagaacaaat gcatgtcgcc tttttgcatt aaatccagca
     6241 acacctctgg cgcggttggg tcagtcactt cataacggca atgtacgcgg ttatccacca
     6301 gcgcatgcgg ttgccaggca tgtacggcac gggcaatttc tgccgcgaat gcattattac
     6361 gcacgcctag ctcgtggttg gcaaatgaca gcgcatgaaa caccgtggtc acaccagcag
     6421 cggcgttgcg cttatcggcc tgcgcgcagg caaagtcgag tggaaaatgc acgttaggac
     6481 gtggctcgac ttctttttcc agcgcatcgc agtgcaaatc aatcatgccg ggaatcaagg
     6541 tttgcccatg caggttaatt tcttccgcat cgctgacttg ttctggatta atagccgcaa
     6601 tcatgccatt ttcaatcagc acggatgcat cgtgcagggt ttcattttct aataccacat
     6661 tggcgtgggt taagtagatt ttgctcatgc atagaccttt tcttgcttgt cgattgcttg
     6721 tgtttttaaa aaggcagatg gtggggtgag ttccaccact cggtcgccta acctgtgggt
     6781 caattccgga tggtgaaaaa tcgccagcat ggcaacacct tcctgtttga tgccctcgat
     6841 aatgccgatt acccgctcgg cggtgacggg gtcaaggctt gccgttggtt catccagcaa
     6901 cagcaggcgt ggcttggcca ccaaaccgcg tgccagattc acacgctgct tctcaccgcc
     6961 tgaaaacgtg gccggtgaga tgctccacaa acgctcaggt aaattgagtg ctgccagcat
     7021 ttctgccgct ttgttgcgtg ctgattcacg gcattcgcca cgcactacca gcggatgcgc
     7081 aaccacatcc agtgtcgatt ggcgcggtaa acaaaacagg aactgcgtga caaagccgat
     7141 ttcttcatgg cgcagttgta gcatgcggta ttcatccgcc tgcgctaaat ccaccagttc
     7201 accatggcgg tcgcgataca gaatttcacc cgcgctaggc agataggtgc ggtaaatgcc
     7261 tttgagtaca gacgatttac ctgcgccagt cgggccgatg agtgcggtca gttcgcctgc
     7321 gtagacttct aaattcacat tatgcgaaga aggaatctgc ttaccttgct catgtaaggt
     7381 aaagtgtttg gcaaagccgt taattttaag tatgggtgtt tgcataaaat ttccttatag
     7441 agcagacgcg accaaacgct gcgtgtaggc gtgttgcggg tcttcaagaa tctggtctgt
     7501 tagcccagat tcaatcacgc ggccatattt catcaccagt gcgcgaccag tcagtaggcg
     7561 aatcacgcct aaatcatgcg tgaccacaat cattgcggtg ttgcgttctt gctggatttc
     7621 taaaatcaaa tccaaaatac gcgcttgcac ggataaatct agccctgttg tcacttcatc
     7681 cagatacagc agcggcggat tggtcgccag tgccttggcg atttgcacgc gctgctgcat
     7741 gccaccagag aatgccgctg gggattcatc catgcgtgac agcagcactt cggtacgtga
     7801 cagcaattct ttggcccgtt cgcggatttc accgtaatgc gatacatcgc tcatcaataa
     7861 tcgctcagca atgttaccgc ctgatgaaat gccaaaattc aggcccaaat gcgggttttg
     7921 atacaccatg ccaaaacgct ggttacgcaa gcgacgttgc tgcaccgcat tgagattaaa
     7981 catttcatgc gtcgcatctt cttcaataaa acgcgcggac ccactggtcg gcgtttggtc
     8041 aaaatacaga tttttaacca cggtggattt accactgccg gactcaccca tcaggcctaa
     8101 aatttcacct tcatgcagat caaaactcac atcatggatt gccactacac tgccgcagtg
     8161 cggacacata ttggtatccg cttcaggtcc ggtggactcg atacagcgcg ggcaaccttt
     8221 gccgtaaatt ttagataaat tacgcacttc taaaatctta cgcatttgcg gcctccttgg
     8281 tttcagctaa caccgtgtcg caataatcgc tgtctgaaca ctggtagcgc ttgctgccat
     8341 cttcattaat atattcatct aaaaaggaat ccgtcgctcc gcagcgctcg catggacggc
     8401 gattgccgtg cgcatcgtta aaaccttcca cgcggaacgg cacatcatca aacgccaatg
     8461 gctcagcttt tgtgtgtggc ggtacggcgt aaattttctt ttctcgccct gcgccaaata
     8521 aaatcaaggc tggcgaatca ttgagcttag gtacatccca acgtggaatc ggtgacgggt
     8581 caatcacgta atgcccattg atacgcgtag ggtaacgatg cgaaatggtg atttcgtcaa
     8641 acttcacgat gtcctcatat aatttcacca gtaggcgcgc gtaatccgcc tcgccatgca
     8701 tcactttgcg tttatcttct gaaggctcta ccaccaccaa cgggtcagga tatggcactt
     8761 gtaacaccaa ggtttggttc ggcgtcagcg gcacttccgg gatgcggtgg cgtgattgaa
     8821 tcagcgtcgc cgcattcaaa tgcgtggtgg tattcacacc tgggcaagtg agctcaacaa
     8881 actggcgtaa attcaccgca ttgaccgagt catcagaacc ctgatcaatc acttttaaag
     8941 tttcatctgc accaatcaac gacagcgtaa tttgcagccc gcctgtacca aaaccacgcc
     9001 ccatcggcat ttcgcgcgat gaatatggta cttggtaacc gggaatagaa atggctttta
     9061 agatagaacg gcgcacttct tttttagcgt gctcatccat aaagccaaag ttataagtca
     9121 cactcattgc gcatctccca tcttggtctt tttgctgtct tcatgttttt gctgggtggt
     9181 acgcaatctg tccatatcag actggaacgt cacgtaatgc ggcattttat agtgcgaagc
     9241 aaagcccatc gaatccacgc catcgacatg caggagcaca aattcagggt ctgaagacgg
     9301 gttactcgcg ccctctttca tgcctttttg caaagcacgg tctaaaatgg ccatagaaat
     9361 ggctttgact tcgttatggc caaaacacgc gccatagccc agcgtaaacg tcggtttgcc
     9421 ttggttgctg tcatcttcat acatcgccac cacttcacat tcagtcatca acacttcgcc
     9481 tgcttctgtg agttcgcccg tgatcgggtg cggcagcatc acaggcaaat accccacgcg
     9541 cagttcggca atggtcgggt gaacatcgcc ataaccgcgc atgttagaat acgccaaggc
     9601 cagcagcgag cctgtttcag cacgcgccat ggtggcaagt gctgccgagc gtggcacggg
     9661 aaaaatcaac ggctcgcgcg taatatcaaa tgccaaagct tcgctgactt ctgcctgcgg
     9721 taacaaccct tcttcacgca atgcctctac cacttttggg aaagtatccg gcagattagg
     9781 ctctggggtt tgttctaaaa actggtgcgc gatgtcacga aatttttcgg gggattcatc
     9841 ggttaaatcc agacgcatca ggcgcaaggc ataatcgtaa gtggggccta acatctgccc
     9901 gcccggaata tccttaaacg ccgatgaaat gcgccgaatc aaacgcatct tgctggtgtc
     9961 ctgcgcttgc gtttccatca ggcgcggcat ggtggagcga taagcccgta aggcaaaggc
    10021 cgcctccagt gtatcgccct gcgattgctt aatcgctagt gctgcaagtt tagggtgata
    10081 taagccccct tctgacacaa tgcgtgaagt aagcaagcgc aactggtttt caatcgtgtc
    10141 gatgctaagc ggcacacccg ccgcgccttc actggcacgc aaggtttcca gtacatcttc
    10201 agccccgcgt atggcctgcc cgcccccttt aatagcgaca taacccataa tttatgctcc
    10261 ctttatgctt gtagtactta tttttgtcgt acgcggcaag gccattacct gtttacctgc
    10321 cagcagcaca atatccacac ccatcggaaa agccgcattc cagctttcac gcgaatcaag
    10381 ccatgcagag ttcaagcctt gcagcgtgac attacgtgcg ccatcaacgc ccgggccaga
    10441 aagggtgtat ggcgtaccat cgttaaacgc agtcacggtg agtaacagcg ttgccccacc
    10501 ctcaggtgac tccagcgtgc caaggctagg cgtaaacgca ggcacttgcc caccctctgc
    10561 cagcacaaag tgcgctgttt cggtggccgc attgactgtt tccaatcgtg ccaaatccag
    10621 ttctgaaatc aggccatgca catcggctat tgaagtagca ccatcgagca gcgtagctaa
    10681 tgtgagtagc aaggcatctt catcgagtgt ttcaatttgt cctgggtttg caaacgcctg
    10741 catcaactgg cgataggcat gctgttgtgt atgcggctgc caaattgttg gtatcgctgt
    10801 tgcaagatta ctcatctgcg tcctcctctt ctgcactgct aagcaatgaa aaatcaaccc
    10861 gagtacgtgc cagcattttt ttacggctgg attgttgttc ggtgagtacg gttaagccac
    10921 gcgccagcaa ctgcgttacc ccttcatggc cgtttagttt tgcactcacg accgcatcca
    10981 taatcgcaat ggcgcgggca agggttgctt cgctgtgcat gatttgcgca gcgccttctg
    11041 tgcggttctg ctgtgcatcc gtgagtacca cgtgggcggt agccagcgga atttccccca
    11101 aaaaatagcc ttcttgcaaa gcgctatctt ttaactgcat caaccctaag ccggcctgcg
    11161 gcaacttaat gtcttctatg gtgtattgct cggcaaagct tgccgccgcg cctatcactt
    11221 catcttctgg caacgccatc agcagtctag gcaactgcgc gcgctggctt tgattattta
    11281 acatttggtt catctctttc ttttagtatg gatgtctaga caagaagtgc cgtgtcgaaa
    11341 cccgctttaa ttggcgggga atcaaccaaa cagtccgatt aaattagttt ggctctcagc
    11401 cttgctgaaa actgatccaa taaaaacacc acaataaaaa tggcaatcaa aatggtcgct
    11461 acatggtcgt actggaacat gtcaaaacgc ccttttagct cttgcccgat accgcctgcg
    11521 ccaaccaaac caatcaccgt tgccatccgc acgttacggt ctaaaatgta gagcgtatag
    11581 gcaatgtatt gcggcactac ttgcggcatc acgccatacc acagcgtttt aagcttgcct
    11641 gcaccaatgg cctccagtgc ttcttgcggt tttttgtcag cgttttcaat gtcttcagca
    11701 tagaacttgc ccaagaaacc tgccgcatgt aaaccaagtg ccaacacacc cgcaatgggg
    11761 ccaaagccat aagccaatac caaaaacaac gccacaatca gctctggcat ggagcgcaac
    11821 aaactaatcg tgccgcgtgc cagcgtgtaa gtgaacttgt tataggtata gttacgtg
//
LOCUS       DAOQSI010000051        11530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_4771_length_11530_cov_6.717908, whole genome shotgun sequence.
ACCESSION   DAOQSI010000051 DAOQSI010000000
VERSION     DAOQSI010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 11530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11530
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4771_length_11530_cov_6.717908"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(461..2386)
                     /locus_tag="PKL42_04840"
     CDS             complement(461..2386)
                     /locus_tag="PKL42_04840"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNU66433.1"
                     /translation="MTNEQTIRNVFEQDGILASQIEGYSARTQQLEMALAIEDAIQHN
                     KQLVAEAGTGTGKTFAYLVPALLSGGKVIISTGTKTLQDQLFSRDLPNVRNALKVPVT
                     VAILKGRANYVCHYHLERAGLEGRFTSREEAGYIPMIKAFAANSKSGDKAELVEVPEN
                     ALVWQSVTSTRDNCLGQECSFYKDCFVMEARKQALAADVVVVNHHLFFADVMLRDEGV
                     TELLPSANTVIFDEAHQLPEVAGLFFGEDVSTSQLLDLARDCTTAYITLAKDCVALGD
                     AIPVLEKACKDFRLVFQYEGSRLPVQKALALKNFESAFEWMQTKLTDLNTVLETQAAR
                     DPMLEKCWQRGMELSAQLSHWLKASNENLVRWVEVFTQSVQLHATPLSVADGFGKQLN
                     AQPRAWIFTSATLAVKSDFSHYQAQMGLQNATTGYWQSPFDYGNQALLYAPANMPDPN
                     SPAYTSAVAAVALPVIQASRGRAFVLCTSLKAMREIHALLKEAFSENGMEYPLLLQGD
                     TTRTELLERFRNHGNAVLVGSQSFWEGVDVRGEALSVVIIDKLPFAPPDDPVLSARID
                     KMNAEGKNAFMEYQLPYSVITLKQGAGRLIRDENDKGVLVICDPRLITKPYGRRIWQS
                     LPPFKRTRELADVAAFF"
     gene            2454..3020
                     /locus_tag="PKL42_04845"
     CDS             2454..3020
                     /locus_tag="PKL42_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNU66434.1"
                     /translation="MRLFIPFQRLAESRLFLCAILAFVSVSCAAVEPAPDVISEAPVE
                     NTMAADETWSEHAREVLMSALSLTGVKYKFGGNSPETGFDCSGFVRYVFNQAASLTLP
                     HSALAISQLGKSIPKSELAPGDLVFFKTVKNAISHVGIYMGNNRFIHSPSAGGNVRVE
                     SMQEGYWAKRYSGAQRLEKSDENTAQSE"
     gene            complement(3186..3470)
                     /locus_tag="PKL42_04850"
     CDS             complement(3186..3470)
                     /locus_tag="PKL42_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SelT/SelW/SelH family protein"
                     /protein_id="HNU66435.1"
                     /translation="MTTHYVEISYCTQCRWMLRAAWLAQELLTTFEEDLASVALKPGT
                     GGVFDIVLDGKLIYSRKTEGRFPEAKEVKQLIRDCIDPNRDLGHSDRPKI"
     gene            3613..5076
                     /locus_tag="PKL42_04855"
     CDS             3613..5076
                     /locus_tag="PKL42_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNU66436.1"
                     /translation="MQVKNKYRGFTKLGLVGSGLLLGFMLSLTYSAVAEKMAKPELPL
                     DDLRAFAEVFGKIKTDYVEPVEDKKLISEAISGMLTGLDPHSSYMDVDSFKDMQAATQ
                     GEFGGLGIEVGMEDGLVKVISPIEDSPAYAAGIKSGDLIMKLDEKPVKGMTLNDAVKI
                     MRGTPNTPIVLTILRKNESKPLIVNLVRAVIKNKSVKFKMIEPGFAYARVTQFQERTG
                     EDLAKAIKTMHAENKGPFKGFVLDLRNDPGGLLNGAVGVSAAFLPKDTLVVYTEGRAQ
                     DSKMNLTVNPDNYARGGAANDYMRDIPADLKTVPMVVLINAGSASASEIVAGALQDHK
                     RAKLIGVRTFGKGSVQTIMPMNNGAAIKLTTARYFTPNGRSIQAKGIEPDFYVEDGTD
                     QSYNIHEADLSRHISNPTDDKSKEAAKAEAAKAAQDAAKAEAAKAKLNEKKYAEPTAP
                     IEPASKDDLQFAQAMNFLKGNPVVESVRPKVETAEAK"
     gene            5206..6381
                     /locus_tag="PKL42_04860"
     CDS             5206..6381
                     /locus_tag="PKL42_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNU66437.1"
                     /translation="MTLTIQELIQEADRCVACGLCLPHCPTYRKTGSEADSPRGRIQL
                     MRAVAQDILPNNDRFKQHIDLCLSCRSCESACPNNVNYGALADTARAVFVPKKRLFSG
                     LVKPFIRHRKLANFALWPLWFLQKTHLLSPLKKLVPTAGFLPNITEPVSWKPLYASSA
                     PKKGAVSLFLGCTSQALDQATLTASITVLNALGFDVHVPTRQTCCGGIARQMGDAEES
                     ARLVATNQNVFDAAMPILTVASGCGAGLCDYLSTHHIQDISAFLSTCDWSDVSLSPLE
                     MRVFVQDPCTLRNVQKSHQAVYQLLKNIPALEVAALPGNSQCCGGAGTYMLNQKAMAT
                     SLLNDKLSAIKANDVVLLATSNIGCSLHIANGLRAQNASVTVVHPVQIIAKQLVFKS"
     gene            6406..7032
                     /gene="coq7"
                     /locus_tag="PKL42_04865"
     CDS             6406..7032
                     /gene="coq7"
                     /locus_tag="PKL42_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HNU66438.1"
                     /translation="MSKLDHFIVEFDTALRTLFAKPHSVRQHPDAAIAEADLSDTEKK
                     HASALMRVNHTGEVCAQALYSGQSLTAHHPEITSALKQAALEETEHLAWCETRIAALG
                     GRTSALNPLFYAGSFTLGAVAGALGDKWNLGFLAETEQQVGAHLASHLELLPETDLKT
                     RSIITQMQQDEAEHATLAKQHGAADLPAPIQFLMKKSSKLMTTVAYYV"
     gene            7064..7381
                     /locus_tag="PKL42_04870"
     CDS             7064..7381
                     /locus_tag="PKL42_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HNU66439.1"
                     /translation="MQKFSDVLLTLEDASHVKRIALFNKDGSPAGVIDNKPGSQGSIK
                     VYYHLYRMYGEISLDAAVEGLSLYAEHTEDAENCPGKHPNVDRLLNILEDEQPLTVEV
                     TEI"
     gene            complement(7580..8005)
                     /locus_tag="PKL42_04875"
     CDS             complement(7580..8005)
                     /locus_tag="PKL42_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNU66440.1"
                     /translation="MHVSIKWIDGVSFIGESETGHAVVMDGAPENGGRNIGMRPMEML
                     LIGMGGCTSFDVVAILKKARQPIVHCVAEIDATRADEIPKVFTKIHVHFVVTGNNLNE
                     TQVERAVKLSAEKYCSASIMLSKSVEITHSFEIKAPDFS"
     gene            complement(8050..8511)
                     /locus_tag="PKL42_04880"
     CDS             complement(8050..8511)
                     /locus_tag="PKL42_04880"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNU66441.1"
                     /translation="MKLLNLLLASGLVFSLGACNADSTQNTSAKETSAMTANLTELQK
                     IDTQVGTGREAEPGFNVKVHYTGWLHDAAAEGGKGKKFDSSVDRGQPFEFFLGQGQVI
                     QGWDEGFAGMKVGGKRTLIIPSEMGYGARGAGGVIPPNANLIFDVELLDVK"
     gene            complement(8613..10052)
                     /gene="dacB"
                     /locus_tag="PKL42_04885"
     CDS             complement(8613..10052)
                     /gene="dacB"
                     /locus_tag="PKL42_04885"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HNU66442.1"
                     /translation="MQFWWCLIVLATGAVGLPAYAELPPSVASALKNAGIPAQNVAVF
                     VQAVEGSSPILSHNADKSMNPASVMKLVTTNAALDLLTPSYRWKTEVYRDGTVTDGVL
                     QGNLIIKGYGDPSFKAQDFWRMLMSVQQTGITKIQGDLVLDKSYFSQQVGPRKTFDDE
                     TWRAYNAEPSALLVNGRNTSFKFVATEAEVNVSQEFELSEIQIINRMKPAKGACGEWR
                     SRFGYTVTQQANGAVVTFNGTYAPDCGERYLELSVFDDEKYAFYTFKKLWCQLGGSFA
                     GKLKTQDVTTQILNGQAVKILAQFSEPLAYVEREINKWSNNLMARQLLLTIGAEKLGA
                     PASEEKGAVAIKDWLASKTLNGQSLAASELVIENGSGLSRIERINAQHLGRMLLSAYI
                     SPVMPEFMASMPILAQDGTIQKRLKESPVAHHAHLKTGSINGVSAIAGYVLDANNHRH
                     VLVMMVNHANAGESKNAQDALIEWVHQQP"
     gene            complement(10058..11362)
                     /gene="bioA"
                     /locus_tag="PKL42_04890"
     CDS             complement(10058..11362)
                     /gene="bioA"
                     /locus_tag="PKL42_04890"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HNU66443.1"
                     /translation="MRNQDLLHRSLQSVWHPCTQMKQHEQFPLVPISRGDGVWLVDED
                     GNRYLDAVSSWWVNLFGHNNTRIKDAIKLQLDTLEHVILAGFTHEPVVALSEKLAKLT
                     GLGHAFYGSDGASATEIALKMSFHYWRNTGKPNKTKFVSLQNSYHGETLGALGVTDVA
                     IFKDTYAPLLMQSAQMPSPDFRLAETGESAENYAMRCAEALENYLAAHHHEIAAFIIE
                     PLVQCAAGMGMYHPAYITRAREICTQYEVHLIADEIAVGFGRTGTMFACEQANIKPDF
                     ICLSKGITGGYLPLSAVLTTDEVYQAFYDDSTIRGFLHSHSYTGNPLACSAALATLAI
                     FESDNVLQTNLEKAAAIWAKMQVLTDLPIKHLRHQGMIFAFDVVTQNAQFFKQCYQAA
                     LQQNLLIRPVGNTVYFMPPYTITFDEINTMVTAAYQAVKNSI"
BASE COUNT         3175 a         2793 c         2494 g         3068 t
ORIGIN      
        1 atttggcgaa cccggacgtt cgacacagac gacacattta tgtcaaaaac atgatatatc
       61 acacaataag atatcaacta tctaatacgg tttcattata cgttgatata ccgaataaat
      121 caaccataaa ttacggtttg taagttagtt acagccaaga ttaaaaacca gcgatcaacc
      181 acggattacg gatacccatg aaatgttaca ttatgctaaa accagtaggg gcttagaacc
      241 aaaattcaag tttaaattta aatcgctacc aagtatatca actggtgatt tttcaatttc
      301 cagccttacc cataaaatta tactcggccc aacattttcc tcgcccatgg caaaaagtac
      361 ttttgaaaaa atgcttacta ttgttggaaa acaagagcta atagaaaaag tgagtgtttc
      421 agatataccc tttaggccat tataaattgc aaattaacct tcaaaaaaac gccgcgacat
      481 ctgctagttc tcttgttcgt ttaaacggcg gcaaactctg ccaaattcgc ctgccataag
      541 gcttagtaat taaacgcgga tcgcaaatca ccagcacgcc tttatcattt tcatcgcgaa
      601 ttaacctgcc cgcgccttgt ttgagcgtaa tcaccgaata cggcaactga tattccataa
      661 acgcgttttt tccttcggca ttcattttat caatgcgggc tgaaagtacg gggtcatcgg
      721 gcggggcaaa aggtaacttg tctataatca ctacactgag cgcttcgccg cgcacatcaa
      781 cgccttccca aaagctctga gagcccacta gcacggcatt gccatggttg cggaaacgct
      841 ctagcaactc ggtgcgcgta gtgtcgcctt gcaaaagtag cgggtattcc atgccgtttt
      901 ctgagaaggc ttctttaagc agcgcatgaa tttcgcgcat ggctttgagc gaggtacaca
      961 acacaaaagc acggccacgg ctggcttgaa tgacaggcaa ggccaccgca gccacggctg
     1021 atgtgtaggc aggcgaatta gggtcaggca tattggcggg cgcatacaat aaagcttggt
     1081 tgccataatc aaacgggctt tgccaatagc cagtagtggc attttgcaag cccatttgcg
     1141 cttgataatg gctaaaatca cttttcaccg ccagcgtggc agaggtaaaa atccaagcgc
     1201 gtggttgagc attaagttgt ttgccaaagc catccgctac gctgagtggc gtggcgtgta
     1261 gttgcacgct ttgggtaaac acttctaccc agcgcactaa gttttcattg ctggctttta
     1321 accaatggct gagttgcgca ctgagttcca tgccgcgttg ccagcatttc tctagcatcg
     1381 ggtcgcgcgc agcttgggtt tctagcacag tgttcaaatc agtgagtttg gtttgcatcc
     1441 attcaaaagc ggattcaaaa tttttgagtg ccaaggcttt ttgcacgggc aggcgcgagc
     1501 cttcatattg aaacaccagc cgaaagtctt tgcaggcttt ttcaagaaca ggaattgcat
     1561 cgcccaaggc cacgcaatct ttagccagcg taatataggc ggtggtgcag tcacgcgcta
     1621 aatccagcaa ctggctggtc gatacatctt caccaaaaaa caagcctgcg acttctggca
     1681 actgatgcgc ttcatcaaaa atcacggtgt tggcacttgg caaaagttca gttacgcctt
     1741 catcacgcag cattacatcg gcgaaaaata agtgatgatt caccaccacc acatcggccg
     1801 ccagcgcttg tttgcgcgct tccatcacaa agcagtcttt ataaaaacta cactcttggc
     1861 ccaggcaatt gtcgcgcgta gacgttacac tttgccacac cagcgcattt tctggcacct
     1921 ccaccagctc ggctttgtcg cctgatttag aatttgcggc aaaagcttta atcattggaa
     1981 tgtaacccgc ttcttcacgc gaggtaaaac gcccttccag ccctgcgcgt tctaaatggt
     2041 agtggcacac ataattggcg cgccctttaa gaatcgccac ggtcactggc acttttaagg
     2101 cattacgcac attgggtaaa tcacggctga aaagctggtc ttgcagcgtt ttagtccccg
     2161 ttgaaataat caccttgccg ccagaaagca gcgcaggcac caaataagca aaggttttac
     2221 cggtacccgt accggcttca gccactaact gtttgttgtg ctgaatggca tcttcaatgg
     2281 ccagcgccat ttctaactgc tgcgtacgcg cagaataacc ctcaatttgg cttgctagaa
     2341 tgccgtcttg ttcaaatacg ttgcggatag tttgctcatt cgtcatgtgc gccattatcc
     2401 catgactgac tagccaagct tgcgtttttc gcgtgaattc ttataatccc cccatgcgtt
     2461 tatttatacc attccaaaga ttggcggaat cacggctgtt tttgtgcgca atattggcat
     2521 ttgtcagcgt ttcttgcgcg gccgttgaac ccgcgccaga tgtcatctct gaagcgcccg
     2581 tcgaaaatac aatggccgca gatgaaacgt ggtctgagca cgcgcgtgaa gtgctgatga
     2641 gtgctttaag cctgactggc gtaaaataca aatttggcgg caactcacct gaaaccggct
     2701 ttgattgcag cggttttgtg cgctacgtat ttaatcaagc cgccagctta acactgccgc
     2761 acagcgcttt ggccattagc caactgggca agtctattcc aaaaagtgaa ctcgcgcctg
     2821 gcgatttagt ttttttcaaa actgtcaaaa atgctatttc acatgtaggc atttacatgg
     2881 gcaacaatcg ctttattcac tctcccagcg cgggcggcaa tgtacgcgtt gaaagtatgc
     2941 aagaagggta ttgggcaaag cgttacagtg gcgcacaacg gctggaaaaa tctgacgaaa
     3001 ataccgcgca atctgaatga gcaaaaagcg ataaaaaccg cttaatcttt ttcgatgctc
     3061 tgcgagcctt ataaataaag gcttccagag cataaatttt ttctcggcaa aatttagtgc
     3121 ttcaaaacaa cttttacaaa cttcttttta gcatttattt tttgcaaaca atttgcaaat
     3181 agcgcttaaa tttttggccg atcgctatgc cctaaatctc gattgggatc gatgcaatct
     3241 ctgattaact gcttcacttc ttttgcttca ggaaagcgcc cttctgtctt gcgcgaataa
     3301 atcagtttgc catctaacac aatatcaaac acgccgcccg tgccgggttt taaggcaacg
     3361 ctggctaaat cttcttcaaa cgtcgtgagc agctcttgcg caagccatgc cgcgcgcaac
     3421 atccagcgac attgcgtgca gtagctaatt tcaacataat gcgtcgtcaa tttggtaatc
     3481 cttactggta tactttgctt agtataaatg aaatataata agtgcaaatt gaaactaatc
     3541 gaactgacct tggctcataa ccatgcgtaa cattagccgg gcgctacaat ctattattgg
     3601 gaatcatcat acatgcaagt aaaaaataaa tatcgcggtt ttaccaaact aggcttggtt
     3661 ggttcgggct tgttgttggg ctttatgtta agcctgactt attctgcagt ggcagaaaaa
     3721 atggccaaac ctgaattgcc gctagatgac ctgcgcgcgt ttgccgaagt gtttggcaaa
     3781 atcaaaacgg attacgttga accggttgaa gacaaaaagc tcatttcaga agccatcagc
     3841 ggtatgctca ctggtctaga cccccactca agctacatgg atgttgattc attcaaagac
     3901 atgcaggcag ccacgcaagg tgagtttggt ggtttaggca ttgaagtcgg tatggaagat
     3961 ggcttggtga aagtgatttc accgattgaa gattcacctg cttatgcggc cggcattaaa
     4021 agtggtgacc tcatcatgaa attggatgaa aaacctgtaa aaggcatgac gctcaatgat
     4081 gcagttaaaa ttatgcgtgg cacacccaac accccgattg ttttaaccat attgcgcaaa
     4141 aatgaaagca aaccgctgat tgtgaactta gtgcgcgcgg tcattaaaaa caaaagcgtg
     4201 aaatttaaaa tgatcgaacc aggctttgct tacgcaagag tgacgcaatt ccaagagcgc
     4261 acaggcgaag acctcgcaaa agcgattaaa accatgcatg cagaaaataa aggcccattc
     4321 aaaggctttg tgcttgattt acgtaacgac ccgggtggct tgctcaatgg tgcggtgggt
     4381 gtttcagcag cctttttacc aaaagatacg ttggtggttt acaccgaagg ccgtgcgcaa
     4441 gattcaaaaa tgaacctgac ggttaaccct gacaattacg cgcgtggcgg tgccgcgaat
     4501 gattacatgc gtgatattcc agcagattta aaaaccgtgc cgatggtggt gcttatcaac
     4561 gcaggctcgg cttccgcttc tgaaattgtg gcgggcgctt tgcaagacca caagcgggca
     4621 aagttaattg gcgtgcgtac ctttggtaaa ggttcagtgc aaaccattat gccgatgaat
     4681 aatggcgcgg cgattaaatt gactaccgcg cgttatttca cgccaaatgg tcgttcaatt
     4741 caggcaaaag gcattgagcc ggatttttat gttgaagacg gtacggacca atcgtacaac
     4801 attcatgaag cggatttatc gcgccacatt agcaacccaa cggacgataa atctaaagaa
     4861 gcagcaaaag ccgaagccgc taaagccgcg caagatgcag caaaagcgga agctgccaaa
     4921 gctaaattga atgagaaaaa atacgccgag ccaacagcgc caattgagcc tgccagcaag
     4981 gatgacttac agtttgcgca ggccatgaat tttctaaaag gcaatccagt ggttgaatct
     5041 gtgcgaccaa aggttgaaac tgccgaagca aaatagcact tcaacaatag gtgatttaaa
     5101 ataaaaatcc gcttcgtgcg gatttttatt ttcaagcgcg ataaatatta tcgaataagc
     5161 attgaacact accaccgagt ttatgcactg tgtttataat gcgatatgac tttaaccatc
     5221 caagaactta ttcaagaggc agaccgttgc gtggcttgcg gcttatgctt accgcactgc
     5281 ccgacctatc gaaaaaccgg ttctgaggcc gattcacccc gcgggcgcat tcagttaatg
     5341 cgtgctgtgg cgcaagatat tttgcccaat aatgaccgct ttaaacaaca catcgattta
     5401 tgcttaagtt gccgcagctg cgaatcagcc tgccccaata acgtaaatta cggtgcgctg
     5461 gccgataccg cgcgcgcagt gtttgtgcct aaaaagcgcc tattttcagg cttagtaaaa
     5521 ccgtttattc gccatcgaaa attagccaat tttgcacttt ggccgctttg gtttttgcaa
     5581 aaaactcacc tgctgagtcc gctaaaaaaa ctagttccca ctgcaggttt tttaccaaat
     5641 attactgagc ccgtttcttg gaaacccctc tacgcttcat ctgcacccaa aaaaggggcg
     5701 gtaagcttat ttttaggctg taccagccaa gcgctcgacc aagccacgct aacagccagc
     5761 attactgtac tcaacgcact gggttttgat gtgcacgtac ccacgcgcca aacctgctgt
     5821 ggcggaattg ccaggcaaat gggcgatgct gaagagtccg cgcgtttagt tgcaacaaac
     5881 cagaatgtat ttgatgccgc catgcctatc ctcaccgttg caagcggttg tggggctggt
     5941 ttgtgtgatt acttgtctac gcaccatatt caagacatca gcgctttttt aagcacttgt
     6001 gattggtctg acgttagcct ttcgccctta gaaatgcgtg tttttgtgca agatccgtgt
     6061 acattaagaa acgtgcaaaa aagtcatcaa gcggtttatc aactgctcaa aaatatacct
     6121 gcacttgaag ttgcagccct gcctggcaat agccaatgct gtggtggcgc cggcacctac
     6181 atgcttaatc aaaaagcaat ggcaaccagt ttattaaatg acaaactttc tgccatcaaa
     6241 gcaaatgatg ttgttttgct ggcaacttcc aatataggtt gcagtctaca cattgccaat
     6301 ggtttgcgcg cgcaaaatgc atctgttaca gtggttcatc cagtgcaaat catagccaag
     6361 cagctagtct ttaagtccta accgaataaa tcaaggagca atcagttgag caagctagat
     6421 catttcattg tggaatttga taccgcacta cgtaccttgt ttgcaaaacc gcacagtgtg
     6481 cgtcagcatc cagatgctgc cattgctgaa gctgatttat ctgataccga aaaaaaacac
     6541 gccagcgcgc tgatgcgtgt caatcatact ggtgaggtat gcgcgcaggc tttatacagc
     6601 gggcaatcgc tcacggcgca tcatcccgaa attaccagtg ctttaaagca ggccgcgctt
     6661 gaagaaaccg agcatcttgc ctggtgcgaa acgcgcatcg ccgcgcttgg cgggcgcacc
     6721 agcgcgctaa accctttgtt ttacgcagga tcattcacct taggcgcagt ggctggggcg
     6781 ttgggtgaca aatggaattt aggatttttg gcagaaaccg aacagcaagt cggtgcacat
     6841 ttagccagcc accttgaact actccctgaa accgacctca aaaccagaag catcattacg
     6901 caaatgcagc aagatgaggc tgaacacgca acactggcaa aacagcacgg tgccgcagat
     6961 ttacccgcac caattcaatt tttaatgaaa aaatcttcta aattgatgac gactgttgca
     7021 tactacgtat aacaaaattt aacaaaagag tggaatcata aaaatgcaaa aattcagcga
     7081 cgtgttactc acattagagg atgcatcaca cgtcaaacgc atcgcattat ttaacaaaga
     7141 tggcagcccc gcaggcgtca ttgacaacaa acccggcagt caaggctcga taaaagtgta
     7201 ttaccacctt taccgcatgt atggcgaaat ttcgctggac gcggcggttg aaggcctgag
     7261 tttatatgcc gaacacacag aagatgcaga aaactgccca ggcaaacacc cgaacgtaga
     7321 ccgcctgttg aatattttgg aagatgaaca accgttgaca gtggaagtga ctgaaattta
     7381 atttttttgc gcgttgtaat ttgctgttag caattgttta agcagcgcgt tttaaccaaa
     7441 ctatttgcat gaatgtttct aaaaaaaaca gaaaaattca cgttattttt agaccaacct
     7501 tttttgatgc tctgcgagcc ttataaataa aggcttccag ggaatttatt ttttctcggc
     7561 gcaaataacc cgttttgcct taactaaaat cgggggcttt gatttcaaaa ctgtgcgtaa
     7621 tttctactga ctttgacaac ataattgaag cagaacaata tttttctgct gagagtttta
     7681 cggcgcgttc cacctgcgtt tcattcaaat tgttacccgt caccacaaaa tgcacgtgaa
     7741 ttttggtaaa cacttttgga atttcatccg cacgtgtcgc atcaatttca gccacacaat
     7801 gcacgatggg ctgacgggct tttttgagaa tggccaccac atcaaacgaa gtgcatccgc
     7861 ccatgccaat caataacatt tccatggggc gcatgccaat attgcgtccg ccgttttcag
     7921 gtgctccatc catcaccacc gcgtggccag tttcagattc gcctatgaaa ctgacgccat
     7981 cgatccattt aatgctaacg tgcattttac tctcctgtag gcgcgcaata aattgcgcgc
     8041 ctacatattc tatttaacgt ctaacaattc aacatcaaaa atcaaattag cattcggtgg
     8101 aattacgcca cccgcgccgc gcgcgccata acccatttca gatggaataa tcaaggtgcg
     8161 tttgccaccc actttcatgc cagcaaaacc ttcatcccag ccttgaatta cttggccttg
     8221 acccaaaaag aattcaaatg gctgaccacg gtcaacgctg ctatcaaatt ttttgccttt
     8281 tccgccttct gcggcagcat cgtgcagcca tccggtgtaa tgtactttga cattaaaacc
     8341 gggttctgcc tcgcgacctg tgcccacctg cgtgtcaatt ttttgtaatt ctgtgagatt
     8401 tgctgtcatg gccgaggttt cctttgctga agtattttgt gtggagtctg cattacaagc
     8461 gccgagtgaa aaaaccaatc cgcttgctag caacaggttc aatagtttca ttgctaatcc
     8521 tttatttcat tcaatcatct aaaattatta caggcaatat gatacctcaa tcgccagtta
     8581 agttcgcctg tatttaccta aggccgctcg ccttaaggtt gttgatgcac ccattcaatc
     8641 agcgcatctt gcgcattttt gctttcccct gcgttagcat gattcaccat catcaccagc
     8701 acatggcggt ggttattcgc atccagtaca tagccggcaa tcgcgctcac gccattgatc
     8761 gagcctgttt ttaagtgcgc atgatgcgcg actgggcttt ctttcaggcg cttttgaatc
     8821 gtgccatctt gtgccaaaat aggcatcgag gccataaatt ctggcatcac tgggcttatg
     8881 taagcactta gtaacatacg ccccaaatgt tgggcattaa tacgctcaat gcgggataaa
     8941 ccagaaccat tttcaatgac cagttcgctc gctgccagcg attgtccatt aagcgtttta
     9001 ctggctagcc aatctttgat ggccacagcg cctttttcct cgctggcagg tgcgcctaat
     9061 ttttccgcac cgatggtgag caataattga cgcgccatta aattattgct ccatttatta
     9121 atctcgcgct caacataagc caatggctca gaaaattgcg ctaaaatttt tactgcctgg
     9181 ccatttaata tttgcgttgt aacatcctga gtttttaatt ttccggcaaa acttccgcca
     9241 agttggcacc ataatttttt gaacgtataa aacgcgtact tttcatcatc aaacacgctg
     9301 agttccaagt agcgctcacc gcaatctggg gcgtaagtgc cattaaacgt gacaactgca
     9361 ccgttggctt gttgcgtgac ggtataacca aaacggctgc gccattcccc acatgccccc
     9421 ttggctggct tcatgcgatt gatgatttgt atttcactca actcaaactc ttggctgacg
     9481 ttaacttccg cctcagttgc gacaaactta aaactcgtgt ttcgtccatt gaccaacaac
     9541 gcactcggct cggcgttata cgcgcgccat gtttcatcat caaaggtctt tctagggcca
     9601 acttgttgcg agaagtagct tttatccaac actaaatcgc cttgaatttt tgtaatgcca
     9661 gtctgttgca cactcatgag catgcgccag aagtcttgtg ccttgaaact tgggtcacca
     9721 taacctttaa taattaaatt gccttgtaac acgccatctg taacagtgcc atcgcgatat
     9781 acttcggttt tccagcggta agatggtgtc agtaaatcga gtgcggcatt ggttgtcacc
     9841 aatttcatca ccgaggcggg gttcatgctt ttatccgcat tgtggctgag tattgggctg
     9901 ctgccttcaa cagcctgaac aaaaactgcg acattttgcg ctggaatacc cgcattttta
     9961 agtgcgctgg caacagatgg aggtaactcc gcataggcgg gcaagccaac agcgcctgtt
    10021 gcaagcacaa tcaaacacca ccaaaattgc atgaatttca tattgaattt tttactgctt
    10081 gatacgcagc tgtaaccatc gtattgattt cgtcaaaagt aattgtgtag ggtggcataa
    10141 aataaacggt attaccaacg gggcgaatga gtaaattttg ctgcaacgcc gcttgataac
    10201 actgcttaaa aaactgcgca ttttgtgtaa ccacatcaaa ggcaaatatc atgccctgat
    10261 gccttaaatg cttaattggc aagtcagtaa gcacttgcat tttagcccaa atggcggcgg
    10321 ctttttcaag atttgtttgt aaaacattgt ctgattcaaa aatcgccaag gtcgccaacg
    10381 cagcactaca agccagtggg ttgccagtat aactatggct gtgcaaaaac ccgcgtattg
    10441 tactgtcgtc ataaaaagcc tgatagactt catccgtggt gagtactgct gacaaaggta
    10501 agtagccccc cgtgatgcct tttgagagac aaataaaatc aggttttatg tttgcttgct
    10561 cacaggcaaa cattgtgccg gtgcggccaa aaccaacggc aatttcatcc gcaattaaat
    10621 gtacctcata ttgcgtacaa atctcacgtg ctctagtgat gtaggctgga tgatacatgc
    10681 ccataccggc ggcacattgc acgagcggct caataataaa cgccgcaatt tcatggtggt
    10741 gcgctgccaa ataattttct aaagcttcgg cacagcgcat tgcataattt tctgcacttt
    10801 ccccagtttc ggctaatcta aaatcagggc taggcatttg cgcagactgc attaaaagtg
    10861 gcgcataagt atctttaaaa attgccacat cggtgacgcc tagcgcgccc agcgtctcgc
    10921 catggtagct attttgcagg ctgacaaact tggtcttatt cggcttgccc gtgttgcgcc
    10981 aataatgaaa actcattttc aacgcaattt cagtcgcact tgcgccatca cttccgtaaa
    11041 atgcatgccc taaacctgtc agtttggcga gtttctcaga taacgccacc acaggctcgt
    11101 gggtaaaacc agccagaatc acgtgttcta aggtatccaa ctgcaattta atcgcgtctt
    11161 taatacgcgt gttattgtgg ccaaacaaat tcacccacca agaactcact gcatctaaat
    11221 aacgattgcc atcttcgtct accaaccaaa cgccatcgcc cctgctaatc ggaaccaaag
    11281 gaaattgttc atgttgtttc atctgcgtac aggggtgcca aacagattga aggctacgat
    11341 gtaaaaggtc ttgattgcgc atggctaaat tgtacgatgt ttttgatatc cacataaatc
    11401 aaatgtcgtc acatgcgatt gtttagtcta aaatcgtggc ttagcataac ttcaacgaca
    11461 cgcatgcaaa aaacccgccg ccatttagaa ctcctcgctc ccgcaaaaaa tgctgatttt
    11521 ggcattgagg
//
LOCUS       DAOQSI010000052        11388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_4866_length_11388_cov_4.381099, whole genome shotgun sequence.
ACCESSION   DAOQSI010000052 DAOQSI010000000
VERSION     DAOQSI010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 11388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11388
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4866_length_11388_cov_4.381099"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            609..899
                     /locus_tag="PKL42_04895"
     CDS             609..899
                     /locus_tag="PKL42_04895"
                     /inference="COORDINATES: protein motif:HMM:NF016268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HNU66444.1"
                     /translation="MAKTKFEWDAEKDALNQEKHGVSFAVAQYAFADLRRVIAKDDTH
                     SQSEERFYCFGEVDGGVLTVRFTYRASIIRIIGAGYWRKGKAIYERENKIHE"
     gene            871..1107
                     /locus_tag="PKL42_04900"
     CDS             871..1107
                     /locus_tag="PKL42_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66445.1"
                     /translation="MSAKIKYTNEPLGDLKVIKDFLPSPAELSYNEEAVKVTLALSKK
                     SIDFFKSEAAKNHTQYQRMIRRLVDAYVESHSPT"
     gene            complement(1638..2126)
                     /locus_tag="PKL42_04905"
     CDS             complement(1638..2126)
                     /locus_tag="PKL42_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HNU66446.1"
                     /translation="MVMSAGCATANPVNDMPPIDPNSAIYEVPVKEGVSYQDLLDSLK
                     SVSAGMNYVSPASFNIAEQMKKRDIDPQGIKEVHAVCNLGMGTEIMLDHPEFIVFAPC
                     RIAIYEKPDANQKLKLYIALDRPTYDLKSIKNPTERAKKSAQELEDGLIKLIDKVRKG
                     DI"
     gene            complement(2260..3186)
                     /locus_tag="PKL42_04910"
     CDS             complement(2260..3186)
                     /locus_tag="PKL42_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HNU66447.1"
                     /translation="MKIHQLRYVHEVAQNKLNISTAAEALHTSQPGVSKQIQLLEEEL
                     GLQIFQRNGKRLVGVTEPGAIVIQLAQRIMREMESIKRVGDEFSNVTTGSLTIATTHT
                     QARYKLPAAVKAFIQMHPEIKLTIHQGSPKQVSEQIVSGEADIGIATESISQFDEILC
                     LPCYQWNRCVVVPHDHPLLQDLPLTLEKIASYPIVTYDYGFTGGTLVGSVFHDAGIEP
                     NVVLTAIDADVIKTYVTLGLGIGLLAQMAYDPVRDSNLAMLDVSHLFPMSTTYLGVRK
                     DAFLRGFMIDFMQLLAPAHSRKEIQRALKNLT"
     gene            3357..4328
                     /locus_tag="PKL42_04915"
     CDS             3357..4328
                     /locus_tag="PKL42_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNU66448.1"
                     /translation="MKIRDLDLSESTLSHDHEALGLPPLERQIAEAAVHPILITASYL
                     AIATLLGLAFFLIFKLFNGHQTSGLDLIKEALHNPIFWNAVIVGLIAQAIDGALGMAY
                     GLTSSSFLLASGASPALATGATHLAEVFTTGISGVSHVKLGNVNKKLFFNLLIPGIIG
                     VLLGAYVITNVDGKAIKPYISGYLLIMGLYILSKAFKHITLRTDIESAKVAPLAVFGG
                     FMDTAGGGGWGPIVTTSLVGAGHNPRTTIGTVNFAEFFLTVISATAFFALLDGQVWVM
                     VAGLVVGGLFSAPFAAYATKHFKTKTLLILVGCLISSVSIYSLSKLF"
     gene            4368..6047
                     /locus_tag="PKL42_04920"
     CDS             4368..6047
                     /locus_tag="PKL42_04920"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008107104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HNU66449.1"
                     /translation="MYQYDEIDQQIVDERVAQYRDQTRRYLAGELTEDEFRPLRLQNG
                     LYIQRQAPMLRIAVPYGMLSSKQLRKLGDIAKKYDKGYGHFSTRQNLQLNWPRLEDTP
                     DILAELATVQMHAIQTSGNCIRNITTDQFVGIAPDEVIDPRAMAEIMRQWSTFHPEFA
                     LLPRKFKIAVSGTKEDRAVCQAHDIGLEFYRDANNQIAIKVWVGGGLGRTPILGTVIR
                     EHLEWQHALTYSEAIIRVYNLHGRRDNIYKARIKILVKALGIDEFRSQVEAEWAHLKN
                     NENTITEAELARVAKHFDAMPYETLAAQNADYDAAVASNPAFAAWVKRCVHPHKQAGY
                     RAVTLSLKPHGSAPGDITSEQMWAVADLADAYSFGELRSSHEQNLILADVKLNDLYPL
                     WEKARANGLATPNIGLLTDIICCPGGDFCSLANAKSIPIAEAIQMQFDNLDYLHDIGE
                     LELNISGCMNACGHHHVGHIGILGVDKDGSEWYQVTIGGKQGNDASIGNVIGPSFSAD
                     EMPNVVQKLIEVYLKERTADERFIDTVRRLGIAPFKAHVYSPKETNTEVTA"
     gene            6044..6661
                     /locus_tag="PKL42_04925"
     CDS             6044..6661
                     /locus_tag="PKL42_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HNU66450.1"
                     /translation="MSSLISLKNDVASVASDNWTLVKLPESQVEERKQAGKVVLFKLT
                     GEQTVTEAQVANTVIPETGNVIVPLSVFIARQQALKTRIAQGEIGVWLATHELLETLT
                     QAQADLNHLPIIAIFVERFADGRIFSLGNLLRTRYGFKNELRAFGDVLRDQLFFLKRS
                     GFTSFVIRADRSAQDAIASLNDFTQPYQGAVDEPRPVFKRYNRGA"
     gene            6662..7438
                     /locus_tag="PKL42_04930"
     CDS             6662..7438
                     /locus_tag="PKL42_04930"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HNU66451.1"
                     /translation="MADHFDSGVTMLKPAANNPATRPALTDALVASVKAKATTTLTLL
                     NNASIEFGANNVTFANSLGAEDMVLTDIILKSALNIEIFSLDTGRLPVETYDLMSATE
                     KHYNTKLNVYFPHADAVETYVKLNGINAFYESIDLRKACCHMRKVEPLQRALKNKAAW
                     ITGMRATQATTRASLPVREFDTANQLEKLNPLSDWTEQEIWAYIRMHEVPYNKLHDQF
                     YPSIGCAPCTRAVAMGEDIRAGRWWWEDPQNKECGLHLKK"
     gene            7488..8414
                     /gene="cysD"
                     /locus_tag="PKL42_04935"
     CDS             7488..8414
                     /gene="cysD"
                     /locus_tag="PKL42_04935"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HNU66452.1"
                     /translation="MNKQPLSHLDWLEAEAIHILREVAGQCSNPVLLFSGGKDSLCIL
                     RLAEKAFRPGKFPFPLLHIDTGHNYQEVINFRDQRAAELGERLIVRSVEDSMKRGTVV
                     LKTPDEPRNKHQSVTLLEAIEEFGFDCCIGGARRDEEKARAKERIMSFRDEFGQWDPK
                     NQRPELWNLYNARSHKGENIRAFPISNWTEMDVWQYIERENLQLPSIYFAHQRDVVMR
                     GGAIVPINVPLANGDVVNPPKTGEEIINMQVRFRTVGDITCTAPVISDADNVSKIVLE
                     TATTTITERGATRLDDQTSDASMEQRKKEGYF"
     gene            8415..9692
                     /gene="cysN"
                     /locus_tag="PKL42_04940"
     CDS             8415..9692
                     /gene="cysN"
                     /locus_tag="PKL42_04940"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HNU66453.1"
                     /translation="MTNKTQNDSLLRFMTCGSVDDGKSTLIGRLLFDTKTILADTLTQ
                     ISNTSKKRGMEAVDLSLLTDGLQAEREQGITIDVAYRYFSTGTRKYIIADAPGHEQYT
                     RNMVTAASTANLAIILIDARRGVLTQTRRHSYLAHLVGIQHIVVAVNKMDLVNYDQAI
                     YDKICADYTAFAKEIGLAQARDIRFIPMSALNGDMLVDRLDSMPWYQGETLLEMLEKA
                     PAAHTEQSEEFRFPVQFVCRPHASADAALHDFRGFMGRVESGDITVGDAVTVLPNGYQ
                     SKVKAIQLGEAQLQSAQTEQSITLLLEDEIDTSRGDMIVKTGSNITPVKQIEAFVCWL
                     SETPMSTARTYIIRHTTRESKAKVGAISYKVDVNTLEQQTTTDLKMNDIARISFKLAQ
                     PLMVDSYSQNRATGAFIVIDESTNNTVGAGMIV"
     gene            9790..10113
                     /locus_tag="PKL42_04945"
     CDS             9790..10113
                     /locus_tag="PKL42_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNU66454.1"
                     /translation="MTYVVTENCIQCKYTDCVDVCPVDCFVEGPNFLAIDPDECIDCT
                     LCVAECPAEAIFAEDDVPANQQEYIALNARLAKVWPVISARKEPLEDADAMNGVPDKR
                     DLLVE"
     gene            10227..10652
                     /locus_tag="PKL42_04950"
     CDS             10227..10652
                     /locus_tag="PKL42_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNU66455.1"
                     /translation="MKDSLKTGIQYTHTFFVPQSKTVPALYPEATEFIAMPEVFATGF
                     LVGFLEWACIKAIHPYLDWPFEQTVGTHINVSHQAATPIGLEVTAHVELIAIDGRKLT
                     FNVEAHDGIDLISTGTHERFIINREKFEAKLATKRNQTT"
     gene            10656..11237
                     /locus_tag="PKL42_04955"
     CDS             10656..11237
                     /locus_tag="PKL42_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HNU66456.1"
                     /translation="MELISNLALAGGLSWASGLRLYITVFAVGMMNKFGYIQLPATLD
                     ILSNPIVIAVAGLLAVIEFLADKIPYVDTMWDSIQTFIRIPAGALLAMGAINTSDPVI
                     ATIAALLGGSLAGATHATKAGSRALINTSPEPVSNIAASFGEESALITGGWLMFAHPA
                     VFIGILCGFIVLLFWLLPKVWRGVKMVLVKLKI"
BASE COUNT         3131 a         2535 c         2532 g         3190 t
ORIGIN      
        1 ttagccaatt cctttgcttt ttctagttct tttttcttta gcaacgcttc actttggtta
       61 gttggcagtc tttttattag cgggcttggg ctactttggc tcgccttggc agcaagcgct
      121 aaagttgcag tcaaacttgg taactttttc cctctggggt tttagcgcat ggcaacattt
      181 gtccttattc acaggctgtg cttttctcac cagcctaacc catcattcaa gcgggacgcc
      241 taggcactat gctgaaatcg atctggtttc ttgtgggggt tgtggttgcg tatttcgtgg
      301 tggtatccga cgcctcgttc aacgcaaagg tattagcgtc gatggtatac gttgctggca
      361 tcgtattgat gcttacaaaa tttgccccaa tcttgggtgt cttgcttcag ggtagcgttg
      421 gcattgccat agctttttgg tttgcatacc ggcgggcatg gaagtgaaca tttcgttttt
      481 gttggctttc tataaattgc ctaacccggc agtcaagtgg gacgcgcctt acttttacgt
      541 tagaactcaa taacattaac ccatataaaa gtatggtaat ttatatgggt atgtattacc
      601 attaacaaat ggcaaaaaca aaatttgaat gggatgcaga gaaagatgca ctaaatcaag
      661 aaaagcatgg tgtttcgttt gctgttgccc agtacgcttt tgcagaccta cgccgtgtga
      721 ttgccaaaga cgacacccac agccaatctg aagaacgttt ttattgcttt ggcgaagttg
      781 atgggggtgt tttaacggtg cgatttacat atcgcgcctc aattattcga attattggcg
      841 ctggttactg gcgcaaagga aaggcaattt atgagcgcga aaataaaata cacgaatgaa
      901 ccgttaggtg accttaaagt aattaaagac tttttaccat cgcctgccga gctttcatat
      961 aatgaggagg ctgtcaaagt gactttggca ctaagcaaaa aaagcattga ttttttcaag
     1021 tccgaggctg ctaagaacca tacacaatac cagcgcatga ttcgccgttt ggttgatgct
     1081 tatgttgaat cacacagccc gacctaaacc atcattcaag cggggctggc taaagccagc
     1141 ccctttattc aaacgttagg cgtcatgaaa tatatatcag aacttataaa gccatttttc
     1201 atctttttgt taatattttt ggctattttt atcgtgtctt caaaaatagt acaaaacaga
     1261 gagagtcagt gtgccaaaaa atgctctcaa gcaggggcaa aaaactactc ttatcacggt
     1321 tttaccggtg gtcttcatat tgatgcagat acttgctcat gcattcatta gaaaatacgc
     1381 ctaacccatc attcaagtgg gacgcctaac ggcgctcatt aattataaaa catctcaaaa
     1441 accgttattt ttcaacacaa cctttttcga tgccctgcga gccttataga taaaggctta
     1501 cagaggcgaa aatttttctc gatgaatttt tgatgcgaat ttgctgtttt tttgcacaca
     1561 aatttatata cattgaatta aatttatctg tggttttgtg cggttttctt gccaacattt
     1621 atgcagattt tacttgctta aatatcgccc ttacgcactt tatcaatcag ttttatcagg
     1681 ccgtcttcaa gttcttgggc agattttttg gcgcgctcgg ttgggttttt aatgcttttt
     1741 aaatcgtagg ttgggcggtc tagcgcaatg tagagtttta gtttttgatt ggcgtcaggt
     1801 ttttcatata tggcaattcg gcatggcgca aatacaataa actctgggtg gtctaacata
     1861 atctctgtgc ccatgcccaa gttgcacacc gcgtgcactt ctttaatgcc ttgcgggtca
     1921 atgtcgcgct ttttcatttg ctcggcaatg ttaaagcttg ccgggctgac gtaattcatg
     1981 ccggctgaca cgctttttag gctatctagc aagtcttgat aacttacgcc ttctttcact
     2041 ggcacttcat aaatggcgct gttggggtct atgggtggca tgtcgttcac cgggtttgcc
     2101 gtggcgcaac ctgcactcat aaccataagt agatacagca aatttaaggg ggtttgcatg
     2161 ttgcgctcct attttgttgt gtgcatcatg gcgcaaattt gcagtttaca ctgtaactac
     2221 agttacataa tttaaagtta gcttaaccat tgcaccacgc taggttaagt tttttaacgc
     2281 acgttgaatt tcttttcggc tatgcgcagg ggcaagcaat tgcataaaat caatcataaa
     2341 gccgcgtaaa aaggcatctt ttctcacgcc taaataagtg gtactcatgg gaaataaatg
     2401 gctaacatct aacatggcca agttgctgtc gcgcacgggg tcataggcca tttgtgccag
     2461 caggccaatg cccaaaccaa gtgtgacata ggttttaatt acgtctgcgt caatggcggt
     2521 gagcaccaca ttgggttcaa tgccggcatc atgaaacacg ctgcccacca atgtgccgcc
     2581 tgtaaagccg tagtcgtaag tcacaatcgg ataactggcg attttttcta acgtcagtgg
     2641 caaatcttgc aatagcggat gatcatgtgg caccacgaca caccgattcc actgataaca
     2701 aggcaggcac aaaatttcgt caaactggct aatgctttcc gtggcaatgc caatatcagc
     2761 ctcgccactg acaatttgct ctgaaacctg ttttgggctg ccttggtgaa tcgtgagctt
     2821 aatttcaggg tgcatttgaa taaatgcttt cactgcggca ggcaacttat agcgcgcctg
     2881 cgtgtgtgtg gtggcaatgg tgagcgagcc agtggtaacg tttgaaaact catcgcccac
     2941 acgtttaata ctttccatct cgcgcataat gcgttgcgct agctgaatga caattgctcc
     3001 cggttcggtt acaccgacca aacgcttgcc attgcgctga aaaatttgca ggcctaattc
     3061 ttcttcaagt agttgaatct gcttactcac cccaggctgc gaggtatgta acgcctcggc
     3121 tgctgtagaa atgttaagct tattttgcgc aacttcatgc acgtaacgta actggtgtat
     3181 tttcatgact taattttaaa cttttatata actattggct atatattaga acaattcatt
     3241 ataaaagcta ttgttaaacg atgttatatt acgcacctca ataaaccgtt gctcagtgcg
     3301 taatttcttt agcaaaagcg cttggtaaat caatattcaa ttttggatga ttcagtatga
     3361 aaattagaga cttagacctc tctgaaagca cactttcgca cgaccacgaa gcacttggtt
     3421 tgccgccgct tgagcgccaa attgccgagg ccgcagttca ccctattttg atcaccgctt
     3481 catatttggc cattgccaca cttctgggct tggctttttt tctgatattt aaattattta
     3541 atggccatca gacgtctggc ctagacctga ttaaagaggc cttgcataac cccatatttt
     3601 ggaatgccgt cattgtgggg ttaattgcac aagcgattga tggcgcattg ggtatggctt
     3661 acggcttaac ttccagctca tttttattgg cttctggcgc ttcccctgca ctcgccacag
     3721 gcgctacgca tttagctgaa gtgttcacca cgggcatttc tggtgtttca catgtaaaac
     3781 ttggcaacgt gaataaaaag ttatttttta acttattgat tcccggcatc atcggcgtat
     3841 tacttggcgc ttacgtcatt accaatgttg atggcaaagc gattaaacct tatatcagcg
     3901 gctatttact gattatgggt ttatacatat tgagcaaagc ttttaaacat atcaccttaa
     3961 gaaccgacat tgaatctgct aaagtagcac ctttggcggt atttggcggc tttatggaca
     4021 cggctggcgg cggtggctgg ggcccaatcg tgacgacttc attggtgggg gcaggacata
     4081 atccgcgcac gaccattggc acagtcaatt ttgccgagtt tttcttaacg gtgatttcag
     4141 ccacagcttt ttttgcacta ctggatgggc aagtgtgggt gatggtggca ggtttagtgg
     4201 ttggcggctt attttcagcg ccttttgctg cttacgccac aaagcacttt aaaactaaaa
     4261 cgctattgat tttagtcggc tgcctgatta gttcagtaag catatacagc ctgagtaagc
     4321 ttttttaatt tcaaaatttt aattttaaat gactgaatca aaattttatg tatcaatatg
     4381 atgaaataga tcaacaaatt gttgatgaac gcgttgcaca atatcgtgac caaacgcgtc
     4441 gctacctagc aggcgaactc actgaagatg agtttcgccc actgcgctta caaaatggct
     4501 tgtatattca acgccaggca cctatgttgc gtatcgccgt gccttacggc atgctttcaa
     4561 gcaaacaatt acgcaaatta ggcgatattg ccaaaaaata cgataaaggc tacggacatt
     4621 tttctacccg ccaaaacttg caattgaact ggccaaggct tgaagatacg ccagatatat
     4681 tggcagaact tgccactgtg cagatgcacg ctattcaaac ctctggcaac tgcattcgca
     4741 acatcaccac tgaccaattt gttggcattg ctccagatga agtgattgac ccacgcgcga
     4801 tggccgaaat tatgcgccaa tggagtacct ttcaccctga atttgcatta ttgccacgca
     4861 aattcaaaat tgctgtttca ggcacaaaag aagaccgtgc cgtgtgccaa gcgcatgata
     4921 ttggccttga attctaccgt gatgcaaata accaaattgc gattaaagtt tgggtaggcg
     4981 gcggtttggg ccgcacgccg attttaggca ccgtgattcg tgaacattta gaatggcaac
     5041 acgccctcac ctatagtgaa gccattatcc gcgtatataa cctgcatggc cgccgcgaca
     5101 acatttacaa agcacgtatc aaaattcttg taaaagcgct cgggattgat gaattccgta
     5161 gccaagttga agctgagtgg gcgcacctta aaaataacga aaataccatc acagaagctg
     5221 aactagcgcg tgttgcaaag cattttgacg cgatgcctta tgaaacctta gctgcgcaaa
     5281 atgcggacta tgatgcagca gttgcaagca acccagcctt tgccgcttgg gtaaaacggt
     5341 gtgtgcatcc acacaaacaa gcaggttacc gagcagtcac gctttcactc aagccgcacg
     5401 gcagcgcacc aggtgatatc acctctgagc aaatgtgggc tgtagctgat ttagctgatg
     5461 cctatagttt tggtgagtta cgctcttcgc atgagcaaaa cctgatattg gccgatgtaa
     5521 aactgaacga cctgtatccg ctgtgggaaa aagcccgtgc aaatggcctc gcaacgccaa
     5581 atattggctt actcacagac atcatttgct gcccgggtgg agatttctgc tcgctcgcga
     5641 atgcaaaaag tatcccgatt gctgaagcca ttcaaatgca gtttgataac cttgattact
     5701 tgcatgatat tggcgagtta gagcttaaca tttcaggttg tatgaatgct tgcggccacc
     5761 accatgtggg tcatatcggt attttgggcg tggataaaga cggttcggag tggtatcaag
     5821 tgaccattgg aggtaagcaa ggcaacgatg ccagcattgg caacgtgatt ggtccatcat
     5881 tctccgccga tgaaatgccc aatgtcgtgc aaaaactgat agaagtttat ttaaaagaac
     5941 gcaccgctga tgagcgtttt attgacactg tgcggcgcct aggcattgcg cccttcaaag
     6001 cgcatgtcta ttcccctaaa gaaacaaaca cagaggtgac ggcatgagta gcttaattag
     6061 cctaaaaaat gatgttgcta gcgtcgctag tgacaactgg actttagtga agctaccaga
     6121 atcacaagtg gaagaacgca aacaggcagg taaagtggtg ttattcaaat taacgggtga
     6181 acaaaccgtg actgaggcac aagttgccaa caccgtgatt cctgaaactg gcaatgttat
     6241 tgtgccttta agcgtattta ttgcccgtca acaggcattg aaaacgcgta tcgcgcaggg
     6301 cgaaattggt gtttggctgg caacgcatga actgttagaa accctcacac aagcacaggc
     6361 cgatttaaat cacctaccca tcatcgcgat atttgtagag cgttttgctg atggccgtat
     6421 cttttcactt ggcaatttat tacgtacacg ttatggcttt aaaaatgaat tgcgcgcgtt
     6481 tggtgacgtg ttgcgagacc aactgttttt cttaaaacgc agcggtttta ccagctttgt
     6541 tattcgtgct gatcgctccg ctcaagacgc gattgccagc ctgaatgatt tcacacagcc
     6601 ctaccaaggc gcagtggatg agccccgccc agtctttaag cgttacaaca gaggtgcata
     6661 aatggccgac catttcgact caggtgtgac gatgctaaag ccagcagcaa ataaccccgc
     6721 tacgcggcca gcgctgacag acgcacttgt tgccagcgtt aaggctaaag caaccaccac
     6781 gctcactcta cttaacaatg cgagcattga atttggtgca aataacgtca cctttgccaa
     6841 tagtttgggt gcagaagaca tggtgctgac tgatatcatt ttaaagagtg cgctcaatat
     6901 tgaaatcttt tctctagata ctggccgctt acctgtagaa acttacgatt taatgagtgc
     6961 cactgaaaag cattacaaca ccaagcttaa tgtttatttt cctcacgcgg atgccgttga
     7021 aacttacgta aaattaaatg gcatcaatgc gttttatgaa tccatcgact tgcgcaaggc
     7081 atgttgtcac atgcgtaaag ttgagccgct acaacgtgct ttaaaaaata aagccgcatg
     7141 gattacgggc atgagagcca cgcaggccac tactagggcc agcctacctg ttagagagtt
     7201 cgacaccgca aatcagcttg aaaagcttaa ccctctcagc gattggaccg aacaagaaat
     7261 ctgggcctac attcgcatgc atgaagtgcc ttataataag ctacacgacc aattttaccc
     7321 aagtatcggc tgcgcaccct gcacacgcgc tgtagccatg ggtgaagata ttcgcgctgg
     7381 acgttggtgg tgggaagacc cacaaaacaa agaatgcggt ttgcacttaa aaaagtaaaa
     7441 taaattaaag caatctatta aattaatcta atatcttatt gaatataatg aataaacaac
     7501 ctttatcaca tctcgattgg ctagaagctg aagccatcca tattctgcgc gaagttgctg
     7561 gccaatgtag caaccccgtg ctgctttttt caggtggtaa agactcgcta tgtattttgc
     7621 gattggccga aaaagccttt cgtccaggaa aatttccatt tccattgcta catattgata
     7681 ctgggcacaa ttaccaagaa gtcatcaatt ttcgtgacca acgtgcagca gaactcggtg
     7741 agcgcttaat tgtgcgctca gttgaagatt cgatgaaacg tggcaccgtt gttttaaaaa
     7801 caccagatga gccgcgcaat aaacatcaat cggtcacttt acttgaagcc attgaagaat
     7861 tcggttttga ttgttgcatc ggcggtgcaa gacgcgatga agaaaaagcc cgcgccaaag
     7921 aacgcatcat gagtttccgg gatgagtttg gtcagtggga tccaaaaaat cagcgtccag
     7981 aactatggaa cctgtacaac gcccgttcgc acaaaggtga aaatatccgc gcctttccaa
     8041 tatccaattg gacagaaatg gatgtttggc aatacattga gcgcgaaaac cttcaacttc
     8101 caagtattta ttttgcgcat cagcgtgatg ttgtaatgcg tggcggtgcg attgtgccaa
     8161 tcaacgtacc attagcgaat ggcgatgtgg taaatccacc taaaacaggt gaagaaatca
     8221 tcaatatgca agtgcgtttt cgcacggtgg gtgatatcac ctgcacggcg ccagttattt
     8281 cagatgccga taatgtgagc aaaattgtgc tggaaacggc caccactacc attaccgaac
     8341 gcggcgctac ccgcttggat gaccaaacct cagacgcttc aatggaacag cgcaaaaaag
     8401 aaggttattt ctaaatgaca aataaaacac aaaacgattc tctattacgc tttatgacat
     8461 gcggcagcgt ggacgatggc aaaagcacgc tgattggtcg cctgttattt gataccaaaa
     8521 ctattttggc agatacgctc acgcaaattt caaacacctc gaaaaaacgc ggcatggaag
     8581 cggtagattt atcattactt accgatggcc tgcaggctga gcgcgagcaa ggcattacga
     8641 ttgatgtcgc ttaccgttat ttcagcactg gaacacgtaa gtacattatt gcggatgcgc
     8701 caggccacga acaatatacg cgcaacatgg tgaccgcggc ttctacagct aacttagcga
     8761 ttattctgat tgatgcacgc cgtggcgtat taacgcaaac aaggcgtcac agctatttag
     8821 cccatttagt gggcattcag catattgttg ttgcagtcaa caagatggat ttagtcaatt
     8881 acgaccaagc catttatgac aaaatttgtg ctgattacac agcgtttgcc aaagaaattg
     8941 gcttggcgca agcaagggat atacgcttta ttccaatgtc tgcgctcaat ggcgatatgc
     9001 tggttgaccg cttagacagc atgccttggt atcagggtga aacactgctt gaaatgctcg
     9061 aaaaagcccc tgctgcccat acagaacaat ctgaagagtt tcgctttcct gttcaatttg
     9121 tgtgtcgccc acatgccagt gcagatgcag cgttacatga ttttagaggc tttatgggcc
     9181 gcgttgaaag tggtgacatt acagtgggcg atgcagttac cgtgttaccc aatggctatc
     9241 aatcaaaagt aaaggccatt caacttggtg aagctcagct acaaagtgcg caaaccgaac
     9301 aaagcatcac gctgttgtta gaagacgaaa ttgatacttc acgcggtgat atgattgtta
     9361 aaactggtag caacattacc cccgtcaaac agattgaggc ttttgtttgc tggctttcag
     9421 aaacaccaat gtctacagcg cgcacctata ttattcgcca caccacgcga gaatctaaag
     9481 caaaagtggg tgcaatttct tataaagtgg atgttaatac gcttgaacag caaacaacaa
     9541 ctgacctgaa aatgaatgat atcgcccgca tcagttttaa actcgcacaa ccactgatgg
     9601 ttgacagcta cagccaaaac cgtgcgactg gcgcgtttat cgtgatagat gaaagcacta
     9661 ataataccgt tggtgcaggc atgattgttt aacatctatt tgatatggcg caacaattaa
     9721 gccatgcaaa taaactaatt ttctaataaa atagcgtttt acgcttttta gctgttaagg
     9781 actgaagaaa tgacctatgt tgtcaccgaa aactgtattc aatgtaaata tactgactgt
     9841 gtagatgttt gcccagttga ctgctttgta gaaggcccaa actttttagc gattgacccg
     9901 gacgaatgca ttgattgcac tttatgcgta gcagaatgcc ctgccgaagc catttttgct
     9961 gaagatgacg ttccggctaa ccagcaggaa tatattgcac tcaacgcgcg tttagctaaa
    10021 gtttggccag tgatttctgc acgtaaagag cctcttgaag atgcagatgc catgaatggc
    10081 gtaccggata agcgtgattt actggttgaa taaactttaa ccttcacatt tttcaccaag
    10141 gaggccatgc ctcctttttt gttgtttgtg catttgtctt tagcaaaaac tatgttgtaa
    10201 tgacattttt tggccaaaag taaagcatga aagactcatt aaaaacgggc attcaataca
    10261 cgcacacgtt ttttgtgcca cagtctaaaa ccgtgccagc tttgtacccc gaagccacag
    10321 aatttattgc catgcctgaa gtatttgcca ctggtttttt agtgggcttt ttagagtggg
    10381 cctgcattaa agccattcat ccctaccttg attggccttt tgagcaaact gttggcactc
    10441 atatcaatgt aagccatcaa gcagctacgc ccatcggttt agaagtcacg gctcatgttg
    10501 aactcatcgc gattgatggc agaaaactta cttttaatgt agaagcacat gatggcattg
    10561 atttaatttc aacaggcacg catgagcgct ttatcatcaa ccgtgaaaaa tttgaggcca
    10621 aattagccac aaagagaaat caaactacct aacatatgga actcatctcg aatctggccc
    10681 ttgcgggcgg cctcagttgg gctagcggac tgcggcttta tatcaccgtg tttgccgtgg
    10741 gcatgatgaa taaatttggc tacatacaac tgcccgctac cttagatata ctcagcaacc
    10801 cgattgtgat tgctgtggct ggcttgctag ctgtgattga atttttagcc gataaaatcc
    10861 cctatgtaga taccatgtgg gatagcattc aaacttttat ccgcatcccc gctggcgcac
    10921 tgttagcgat gggcgcgatt aacaccagcg accctgttat cgccaccatc gcagccctac
    10981 tcggcggctc gcttgcaggc gcaacgcacg ccaccaaagc aggcagccgc gcattgatta
    11041 acacctcacc tgagccagtc agcaatatcg ccgcctcgtt tggtgaagaa agcgcgctga
    11101 ttacgggcgg ctggttgatg tttgcccacc ctgcggtgtt tataggcatc ctttgcggat
    11161 ttattgtatt gctgttttgg ctattaccta aagtttggcg aggggtgaaa atggtgttgg
    11221 ttaagttgaa aatttaacac ggaaaactaa cccataaaat gaaagacaaa cctatttcta
    11281 gttatcgcat cagcgaatat gccaaaaaat ttgccagcta cagcaaatac cattcaattg
    11341 atattcacga aaataatatt gtctaactag agcagtgtaa aatttaat
//
LOCUS       DAOQSI010000053        11269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_4957_length_11269_cov_5.268771, whole genome shotgun sequence.
ACCESSION   DAOQSI010000053 DAOQSI010000000
VERSION     DAOQSI010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 11269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11269
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4957_length_11269_cov_5.268771"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="PKL42_04960"
     CDS             complement(<1..927)
                     /locus_tag="PKL42_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HNU66457.1"
                     /translation="MDNVPIPLQLGALVLLLLISAFFSIAETSLMSLNRYRLRHLVKE
                     GHRGAKLANALLAKTDKLLGVILLCNNFANAASATLVTVIIVELFGEGEWMLMLGTVA
                     VTFAILVFSEISPKVIAAAHAEKLGILCSYILYPLLKVLYPVVWFVNLFVEGLLKILG
                     VNVNFNETSQSLTMDELRSIVTDAGNFLPKKHRTILLNLFELEKISVDDVMTAHTLVE
                     AIDFDAPLEDILQQIANSQHTRLPVRQGEHEEIIGILHLRKVMSQLRAHRESNDLNKE
                     TLREVITEPYFIPSGTPLYTQIQQFQEHQQRVA"
     gene            complement(1189..2127)
                     /gene="secF"
                     /locus_tag="PKL42_04965"
     CDS             complement(1189..2127)
                     /gene="secF"
                     /locus_tag="PKL42_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNU66458.1"
                     /translation="MELFRIKNDIPFMSYGRLTTTISLVTFILAVFFLATRGLNFGVD
                     FTGGTIMEVSYPQAANLDTIRKAVDNIGLKEATVQNFGSSRDVLIRLPAKTDISVANL
                     SEHVKEALVSADPAAEVRRVEAVGAQVGAELYENGALALFFVIVGIMLYLWLRFEWRF
                     AVAAIIANMHDVVIILGFFAFFQWDFNLTVLAAILAVLGYSVNESVVVFDRVRENFRK
                     MRKASVVQVIDNAITRTMSRTIITHFSTQLMVLSMLLFGGEALHNFALALTIGILFGI
                     YSSVLVASPIAMYLGVSRANLMGDTEKKPEAAEATP"
     gene            complement(2153..4033)
                     /gene="secD"
                     /locus_tag="PKL42_04970"
     CDS             complement(2153..4033)
                     /gene="secD"
                     /locus_tag="PKL42_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNU66459.1"
                     /translation="MNRYPMWKNILIAVIILIGLIYTVPNFFGESPAVQITPAKSTTK
                     LDTALLGQVEEIFKKQNVAYDGVYLDERGVKARFANTDTQIRAKDVLEAHLGKDFTVA
                     LNLLSRSPDWLRGSFNAKPMYLGLDLRGGVHFLMQVDMKAVLKKAVEGYVGDFKASLR
                     KERIAYTSVSNEGQEIQIRFNDANELAKAKKLIGKNYADLTLTDTTLGEDKVLKITLS
                     LVEQKALQDAAIKQNIQTLHNRVNELGVAEPVIQQQGADRIVVQLPGVQDTAKAKDII
                     GRTATLQVRMVAAHGLNNGSRDYNPQKVEAAINGTVPAGTELLKVRGDRTGQEEPLLV
                     DKEVVFSGDNLTSANPNTDSQQGGAIISIRLDAKGAAAMKSASREGVKRRMAVVLIEK
                     NEQEVLTAPEIQTELSSSFQITGMANLQEAQDLALLMRAGALAAPMDIIEERTVGPSM
                     GEENISRGIHSTLWGFAAIAIFMVIYYHLFGGVSVFALSINLLLLVALLSMLQATLTL
                     PGLAAVALTLGMAIDANVLINERIREELRNGNTPQAAIHAGYERAWDTILDSNVTTMI
                     AGLALFMFGTGPVKGFAVVHVLGILTSMFSAVLVSRAVVNLIYGYRRKVGKISIGQIF
                     KA"
     gene            complement(4138..4455)
                     /gene="yajC"
                     /locus_tag="PKL42_04975"
     CDS             complement(4138..4455)
                     /gene="yajC"
                     /locus_tag="PKL42_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNU66460.1"
                     /translation="MFISNAYAASAAPSNDLMSFLPLIVIFVLFYFMIIRPQMKAAKE
                     QRAMIAALQKGDEVVTSGGIVGKVTKVAEAFVTIEIAANTEITVQKQAIQTALPKGTI
                     KSL"
     gene            4846..5460
                     /locus_tag="PKL42_04980"
     CDS             4846..5460
                     /locus_tag="PKL42_04980"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNU66461.1"
                     /translation="MKRSIVAIIFASLSLFTTLVSAETVTIDFESLTSPDLVYKSTYT
                     EDGYRFISSKSDPEAFLSWQTGSPYYSGTAFANNFMGETTTLSKLDGELFNFNSIDLA
                     GVYLNDFFIPGSVLKFNGLLVGGSTVSQDVKINNPGVFTTFKLEGFSNLVSVSWTQGS
                     TAMQNAHQFDNLVLSAAPVPEPSTWILMAVGLIGMISLRCRKII"
     gene            complement(5572..6672)
                     /gene="tgt"
                     /locus_tag="PKL42_04985"
     CDS             complement(5572..6672)
                     /gene="tgt"
                     /locus_tag="PKL42_04985"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNU66462.1"
                     /translation="MQFTLHKQDGLARRGTVKLAHGNVETPAFMPVGTYGTVKAMSPL
                     ELKEIDAHIVLGNTFHLWLRPGLEVIAAHGGLHKFMGWDKPLLTDSGGFQVFSLGAMR
                     KISEEGVKFKNPINGDTCFLTPEESMRIQKVLNSDIVMIFDECTPFPATLKEANDSMQ
                     LSLRWAKRSKDAHQGNPNALFGIIQGGMYEELRDVSLAGLIEIDFDGFAIGGLSVGEP
                     KEDMLRILAHTAPKMPQNKPRYLMGVGTPEDLVDAVTLGIDMFDCVMPTRNARNGWLF
                     TQYGDIKIKNASYKLDTQPLDADCTCYTCQNFTRAYLHHLHKVGEILGARLNTIHNLH
                     YYQVLMQGMRQAIENGTFEAFKTEFAAKRARN"
     gene            7044..7679
                     /locus_tag="PKL42_04990"
     CDS             7044..7679
                     /locus_tag="PKL42_04990"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018228023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNU66463.1"
                     /translation="MNETDQNRRQLATRSAGWATWLGATVAKTNISPNQISVLSIVPA
                     LLGAWLLACTQIDKTPAILLLIVLCIQLRLLCNMLDGLVAIEGGKQSKVGILYNEIPD
                     RIADSLLIVALGYAISMPWLGWLGALLAAKTAYIRLLGGANGLAQDFRGPLAKQHRMF
                     AMSLGCLIAAVEWYVSRTQFALVITAYVIAIGAGLTCITRTLAIAQKLKQQ"
     gene            7692..8309
                     /locus_tag="PKL42_04995"
     CDS             7692..8309
                     /locus_tag="PKL42_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNU66464.1"
                     /translation="MLSNVVRVSLIGLVKLLIGAHPRWIEAAPDLRQTPAQSIYFTNH
                     SSHLDTWALWVALPAVMRARTRPVAAKDYWGNGVIKPFIALKVLNAVLIEREVSRHRD
                     PLAPLLAALAEGDSLIIFPEGTRRAQAEPGNFKGGLFHLAEAFPAVKLVPVYLDTLHR
                     SMPKGSWLPVPLICTVRFGSAMALQANEHKKVFLNRAREAILKLI"
     gene            8320..9300
                     /locus_tag="PKL42_05000"
     CDS             8320..9300
                     /locus_tag="PKL42_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNU66465.1"
                     /translation="MANFLLNLDPNHQFYCLVGGVAILLLIASSIGWVLKSRLNQQPN
                     PSERSRKTIDNLVVRVNAWWVMLAVLVVAFLGGTLGTVILFGAISYFALREFITLTPT
                     RTGDHRTLSLAFFLLIPLQYYLIYIEWYALFTILIPVYAFLLMPCISVLAQDTEHFLE
                     RAAKIQWGVMIAIYCISHAPALLLLKIPNYDGHNTLLLFYFLLIVQLSDVLQYVFGNL
                     FGKTKVAPIVSPNKTLEGLIGGGLSAVCVGALLWWITPFTPLQSAGMAAVIVIMGFLG
                     GLVMSAIKRSLGAKDWGNMIEGHGGMLDRMDSVSFAAPIFFHLVRYFFTA"
     gene            9365..10159
                     /gene="pssA"
                     /locus_tag="PKL42_05005"
     CDS             9365..10159
                     /gene="pssA"
                     /locus_tag="PKL42_05005"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HNU66466.1"
                     /translation="MAEQNDHNKPHEQHKPDATGLRKRGIYLLPNLFTSAALFAGFFA
                     IVQAMNSHFELSAIAIFIAMVLDGLDGRVARLTNTQSAFGAEYDSLSDMVSFGVAPAL
                     ILYVWALKPLGKLGWLAAFAYCACAALRLARFNTKLDDEHQDKRFFQGLPSPGAAALL
                     AGFVWVSYEYGVSGRDIFFGLLQWKWMAWFITVFAGISMVSDLRFYSGKDINLKESVP
                     FFVMLGVMMAFVLISYRPPEVLFFVMVAYALSGYVLWIRDKLKKKA"
     gene            10487..>11269
                     /locus_tag="PKL42_05010"
     CDS             10487..>11269
                     /locus_tag="PKL42_05010"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNU66467.1"
                     /translation="MNQTAEKQNQIIIFDTTLRDGEQSPGAAMTKEEKIRIARQLEKL
                     GVNVIEAGFAAASPGDFDAISEIAKIIKNSTVCSLARASENDVRRAGEAIKHAASGRI
                     HTFIATSKIHMENKLRMTEDQVVERAVQAVKWAKEYTDDVEFSAEDAVRSEIDFLVRV
                     FDEVIEAGAKTINVPDTVGYSIPAPWGERMATLIKRVKNSDKVVWSTHCHNDLGMAVA
                     NSLAAVMGGARQVECTINGLGERAGNASLEEVVMAIKTRRDIF"
BASE COUNT         2987 a         2405 c         2691 g         3186 t
ORIGIN      
        1 cgctacacgc tgctggtgtt cttgaaactg ttgaatttgc gtataaagcg gtgtacctga
       61 tggtataaaa taaggctctg taatcacttc acgcagggtt tctttgttta aatcattgct
      121 ctcacgatgc gcgcgcagtt gcgacatgac tttgcgtaaa tgtagaatgc cgatgatttc
      181 ttcatgttca ccttgtcgca ccggcaagcg cgtatgttgg ctgttggcta tttgctgcaa
      241 aatatcttcg agcggtgcat caaaatcaat ggcttctacc aatgtgtgag ccgtcatcac
      301 atcatctaca ctaatttttt caagttcgaa cagatttaat aaaatagtac ggtgtttttt
      361 aggtaaaaaa ttacctgcat cggtcacgat gctgcgtagc tcatccatgg taagtgattg
      421 agaagtctca ttaaaattga catttacgcc cagaattttg agtaagccct ctacaaaaag
      481 attaacaaac caaactacgg gataaagcac tttgagtaat gggtaaagaa tgtagctgca
      541 aagtatgcca agtttttctg cgtgagctgc agcgataact ttaggcgaaa tttcactaaa
      601 tactaaaata gcaaatgtta ctgctacagt acccaacatg agcatccact ctccttcacc
      661 aaatagctca acgatgatga cggtgactaa tgtagctgag gcggcgtttg caaaattatt
      721 gcatagtaaa attacaccga gcagcttgtc agtttttgcc agtaatgcat tagccaattt
      781 tgccccacgg tgtccttctt tgactaaatg gcgcaatcgg taacgattta gtgacattaa
      841 gcttgtttcc gcaatagaga aaaaagcgga aatcaggagt agtaaaacaa gtgcgccaag
      901 ttgcagcgga ataggaacgt tatccaaggc ggtagctaga tataaaaagt ggagttattt
      961 tacatggaag cgcccaccta aacaatggtt ttgctttttg aagaaattat caaaatctgt
     1021 aaatgaaatc gtaaaaaata aggaaaaaat attgtaaaca acacaagtga aagcactaat
     1081 tctacgagaa aattttaatg ccctgggagc cttgtaaata aaggctcgca gggtatgtca
     1141 aaagggttgt gtggcaatat tgtttgatta ttattttttt gacgtgcttt agggcgttgc
     1201 ttcagcggcc tctggttttt tctcagtgtc gcccattaag ttggcacgac ttacaccaag
     1261 gtacatggca attgggcttg cgaccaatac cgatgagtag atgccgaata aaataccaat
     1321 ggtgagggcg agggcaaagt tgtgtaatgc ttcgccgccg aacaagagca tggataacac
     1381 catcaattgt gtagaaaagt gcgtgatgat ggtacgtgac atggtgcggg taatggcgtt
     1441 gtcgatgact tgcactacgc tggctttacg catttttctg aagttttcac gtacacggtc
     1501 aaacaccacc acagattcat tcactgagta gcccaatact gccaatatgg cggctaatac
     1561 ggtgaggttg aaatcccact ggaaaaacgc gaaaaagcct aaaattatca ccacgtcatg
     1621 catgttggcg atgatggccg ccaccgcaaa gcgccattca aaacgcagcc acaggtagag
     1681 cataatgccg acaatcacaa aaaacagggc aagcgcaccg ttttcataaa gttcagcacc
     1741 cacttgcgcg cctacggctt ccactcggcg aacttcagcg gctgggtcag cagagacgag
     1801 cgcttctttg acgtgttctg agagattagc aaccgagatg tctgtttttg ccggtaagcg
     1861 aatcagcacg tcgcggctcg agccaaaatt ttgcacggtg gcttctttaa ggccaatatt
     1921 atcaaccgct ttgcgaatag tatctaaatt ggccgcttgc gggtagctta cttccataat
     1981 cgtgccgcca gtgaaatcca caccaaaatt aaggccacgc gtggctaaaa agaataccgc
     2041 cagaatgaaa gtcactaatg agatcgtcgt cgtgagacgg ccatagctca taaagggaat
     2101 gtcattttta attctaaata attccataat atttactcta agtaattgac gcttaagctt
     2161 taaaaatttg accgatggag attttgccaa ccttgcgacg gtagccgtag atcaagttca
     2221 ccacagcgcg tgacaccagc accgcactaa acatcgaagt taaaatcccc aaaacgtgga
     2281 ccaccgcaaa accttttacc gggccagtac caaacataaa caaggctagc cccgcaatca
     2341 tggtggtaac gtttgaatct aaaatagtat cccaggcacg ttcataacct gcatgaatgg
     2401 cggcttgcgg cgtgttgccg ttgcgtaatt cttctcgaat acgctcgtta atcaatacgt
     2461 tggcatcaat cgccatgcct aatgtgagcg ccaccgcggc taggcccggc aatgtcagcg
     2521 tggcttgtaa catggataac aaagccacca gtaatagcaa gttaatgctt aacgcaaaca
     2581 cggaaacgcc accaaacaag tggtagtaaa tcaccatgaa aatagcaatg gcggcaaagc
     2641 cccaaagcgt tgagtgaatg ccgcggctga tgttttcttc acccatgctt gggccgacgg
     2701 tacgctcttc aataatatcc attggtgcag ctaacgcgcc agcgcgcata agcaaggcta
     2761 agtcttgcgc ttcttgcaag ttggccatgc cagtgatttg aaagctggaa gatagctcgg
     2821 tttgaatttc aggtgcggtt aatacttctt gttcgttttt ttcaatcagc actacagcca
     2881 tgcggcgttt cacaccttca cgtgaggcgc ttttcatggc ggcagcgcct tttgcatcca
     2941 agcgaatact aataatggcg ccaccttgtt gtgaatcagt atttgggttg gctgatgtta
     3001 ggttatcccc tgaaaacact acctctttat caaccaataa cggctcttct tggccggtac
     3061 ggtcgccccg aactttgagt aattcagtgc cagctggaac cgtgccgtta atcgctgctt
     3121 caactttttg cgggttgtag tcgcgtgagc cgttattgag gccatgtgcc gccaccatac
     3181 gcacttgtag tgttgcggtg cggccaataa tatctttggc ttttgcggta tcttgtacac
     3241 cgggcagttg caccacaatg cggtcagcgc cttgttgttg aatcaccggc tcggccacac
     3301 caagttcatt cacacggttg tgcaaagttt gaatgttttg cttaatggcg gcatcttgta
     3361 aggctttttg ctcaaccaag cttaaagtga ttttcagcac tttatcttca cccaaggtgg
     3421 tgtctgttag cgttaaatcc gcatagtttt tgccgatgag ctttttagcc ttggcaagct
     3481 catttgcgtc attaaagcga atttgaattt cttgcccctc gtttgaaacg ctggtgtagg
     3541 caatgcgctc tttacgaagc gatgctttaa agtcacccac gtaaccttca accgcttttt
     3601 tgagtacggc tttcatgtcc acttgcatta aaaagtgcac gccgccacgc aaatcaaggc
     3661 ccaagtacat gggtttggcg ttaaaagacc cacgcaacca gtcaggagag cgcgatagca
     3721 aattcagtgc cacggtaaaa tctttgccta aatgcgcttc taacacatct tttgcacgaa
     3781 tttgagtatc cgtattggca aagcgcgctt taacgccacg ctcatctaaa tacacgccat
     3841 cgtaggcaac gttttgtttt ttaaatattt cttcaacttg gcccaacagc gcggtatcaa
     3901 gctttgttgt gcttttagct ggcgtaattt gtaccgcggg cgattcacca aaaaaattgg
     3961 gcacggtata aatcagccca atcaaaatga tgactgcgat aagaatgttc ttccacattg
     4021 gatagcggtt catagtctag ctctacttaa gtaacagggt taagttgaca aaaatactag
     4081 gatagcgggt gttattgtgc gctttttatt aaagcgagca ttcattaaaa gtgattatta
     4141 aagactttta atagtgcctt taggcaaggc ggtttgaatg gcctgttttt gcacagtaat
     4201 ttcagtattt gcagcaattt caatggtgac aaaagcctca gccactttgg tcactttacc
     4261 cacaatgccg ccgctggtga ctacttcatc acccttttgc aaagcggcaa tcatcgcgcg
     4321 ttgctctttt gcggccttca tttgcgggcg aataatcata aaataaaata aaacaaaaat
     4381 cacgataagc ggtaaaaagc tcattaaatc gttacttgga gcagcgctgg cggcgtaggc
     4441 gttagaaata aacatattaa aaccttcaaa aaaaagagac acaaaaatta acgcaagatt
     4501 ctaacacatc cttgagtttt gttagtacga tgagcaatag tcaaaaattt tgtgaaagta
     4561 gctttgtgaa tagtggattt ttttgaacac taatcaaatt aatcgtgtct gaccccattt
     4621 aatatcaccg cttgagtcaa agttttagcg aaagtgactt tgtgtcaatt ggtaaaatgc
     4681 agaaacacaa gcatgtcgcg cgtggcaagt ttaagttttt caagagggtt tgtttcgctg
     4741 atacgtgttt atgttgcagt gaatgtagcc taaatgggta attgtatttt cagtatttat
     4801 ttgatatgtt tgattatatt ttttccgcat cgaaaggtat tttcaatgaa aaggtcaata
     4861 gttgccatca tatttgcatc actttcacta ttcactacct tagtctccgc ggaaactgtc
     4921 accattgatt ttgagtccct gactagtcct gatctcgtct ataaatctac ttatactgaa
     4981 gatggctata gatttatctc ttcaaaatcg gatcctgagg cctttttgtc ttggcaaact
     5041 ggcagtcctt attattcagg tacagcattt gcgaataatt ttatgggtga aacgacgaca
     5101 ctatcgaagt tagatggtga gttgtttaac tttaattcga ttgatttagc aggagtgtat
     5161 ttaaatgatt tttttatacc tggctctgtt ttaaagttta acggcctgct ggttggtggc
     5221 agcactgtga gtcaagacgt taaaattaac aatcctggtg tttttactac atttaagcta
     5281 gaaggtttta gcaatcttgt tagcgtttct tggacgcaag gctctacagc aatgcaaaac
     5341 gcacatcaat ttgacaattt agtccttagt gctgcacccg taccagagcc aagcacatgg
     5401 atattgatgg cagtagggtt gataggcatg attagtctac gttgcagaaa aattatttaa
     5461 aagtaaaaaa catcaaaaaa gcgaaggttg gcgtggtttt ttcaggaatg ttctagtccg
     5521 cgtaggtagg ctagtacaat gaagtcaaat aattaactac agtcatgcta cttaattacg
     5581 cgcacgtttt gcggcaaact cagttttaaa tgcctcaaac gtgccgtttt caatcgcttg
     5641 gcgcatgcct tgcatcaaca cttggtaata atgcaagttg tggatggtgt ttaagcgcgc
     5701 gcctaagatt tcgccaactt tatgtaaatg gtgcaagtag gcgcgggtga agttttggca
     5761 ggtataacaa gtgcagtcgg cgtcaagcgg ttgggtgtct agcttgtagc tggcgttttt
     5821 aatttttata tcgccatatt gggtgaatag ccagccgttg cgggcgttgc gggtgggcat
     5881 cacgcagtca aacatgtcaa tgccaagggt cactgcatct accaagtctt caggtgtgcc
     5941 aacgcccatt aagtagcgcg gtttattttg cggcattttt ggtgcggtgt gcgccaaaat
     6001 gcgcagcatg tcttctttgg gttcgccaac ggataatccg ccaatggcaa agccgtcaaa
     6061 atcaatttcg attaagcccg ccagcgaaac gtcgcgcagt tcttcataca tgccgccctg
     6121 aataatgcca aagagcgcat tggggttgcc ttggtgtgcg tctttgctgc gtttggccca
     6181 gcgcaaactc agttgcattg aatcattggc ttctttaagc gtggcaggaa aaggcgtgca
     6241 ttcatcaaaa atcatcacga tgtcggaatt taataccttt tgaatacgca tggattcttc
     6301 aggcgttaaa aagcaggtgt cgccgttgat ggggttttta aacttaacgc cttcttcact
     6361 gattttgcgc atggcgccta agctaaagac ctgaaaaccg ccagagtcag tcaaaagggg
     6421 cttgtcccag cccatgaatt tgtgtaatcc gccgtgtgcg gcaatgactt ctaagcctgg
     6481 gcgtagccat aaatgaaacg tattgcccag cacaatgtgt gcatcaattt cttttagctc
     6541 aagcggcgac atggctttga ccgtgccata ggtgccaact ggcataaaag cgggggtttc
     6601 aacgttgccg tgcgcgagct taacggtacc acggcgcgcg aggccgtctt gtttgtgtag
     6661 ggtaaattgc atgaataacg tgtttaaaat ttgacaaata atgatagccc gcgatattaa
     6721 cacatggcac catcaatact ttgcggtggc gtttgataaa gtaattttaa tgctaaaaat
     6781 ttacctgtaa tttagttaat ataattgctt aaaatttaag cattttttgt gcgaggaaat
     6841 ttttatgctc tgcaagcctt tatttataag gctcgcagag catcgaaaaa ggttgtgcaa
     6901 aaaattagct ttaaaaacat gctttttttc attgatgatg cacggtttat ttgagctgtt
     6961 aagcttgcga ataaaagcac cgcctgcgca acacattcgc cgtataaaca gctacaatcg
     7021 acaaaatttt tgggaaagct gctatgaatg aaaccgatca aaatcgtcgt cagttggcca
     7081 cgcgcagtgc cggttgggca acgtggcttg gggcgacagt tgcaaaaacc aatatttcgc
     7141 cgaatcaaat ttcagtattg agcattgtgc cggcattact gggcgcgtgg ttgctggctt
     7201 gtacgcaaat tgataaaacg cctgcgattt tgttgctcat cgtgctgtgt attcagctgc
     7261 gtttgctgtg caatatgctg gatggtttgg tggcgattga gggcggcaag cagtcaaaag
     7321 tgggcatttt atataatgag attccagacc ggattgctga ttcgttgttg attgtggcac
     7381 ttggttatgc aatctctatg ccctggctgg gctggctggg cgctttattg gccgcaaaaa
     7441 cggcttatat tcggctactg ggcggtgcca acgggctggc gcaagatttt cgcgggccgt
     7501 tggccaagca gcaccgcatg tttgccatgt cgctcggttg cctgattgca gcggtggagt
     7561 ggtatgtgag ccgcacgcaa tttgcgctag tgattaccgc gtatgtgatt gcaattggcg
     7621 cagggcttac gtgcatcaca cgcacattgg caattgctca aaaacttaag cagcaatagc
     7681 ggataaaatc catgttaagt aatgttgtgc gcgtcagcct gattgggctt gtgaaactgc
     7741 tgattggcgc gcacccgcgc tggatagaag ccgcgccaga tttaaggcaa acgccagcac
     7801 aaagtattta ttttaccaat cactccagcc atttagacac ttgggcgctg tgggtggcct
     7861 tgcccgccgt gatgcgcgct agaacaaggc ctgtggcggc aaaggattat tggggcaatg
     7921 gcgttattaa gccttttatt gcgctaaaag tactcaatgc cgtgctgatc gagcgtgagg
     7981 tgtcgcgcca tcgtgacccg cttgcaccat tgttggcggc tttggctgaa ggcgattcgt
     8041 tgattatttt tcctgaaggc acgcgccgtg cgcaggccga gccaggtaac tttaaaggtg
     8101 gcttgtttca tttggcagaa gcttttccag cggtgaagtt agtgccggtt tatttagata
     8161 cgctgcatcg cagtatgccc aaaggcagct ggctgccagt gccgttaatt tgcacggtgc
     8221 gttttggcag tgcaatggcg ctacaggcaa acgagcataa aaaagtattt ttaaatcggg
     8281 cgcgtgaagc cattttaaaa cttatttagg atcatacgca tggccaattt tttgctgaat
     8341 ttagacccaa atcatcaatt ttactgcttg gtcgggggcg tagccatttt gctattgatt
     8401 gcatctagca tcggttgggt gttgaaatcg cgcttgaacc aacagcccaa ccccagcgaa
     8461 agatcacgca aaaccattga taatttagtg gtgcgcgtga acgcttggtg ggtaatgctg
     8521 gcggtgctgg tggtggcatt tttgggcggc acactcggca cggtgatttt atttggcgcc
     8581 atttcatatt ttgccctgcg cgaatttatt acactcacgc ccacgcgcac aggcgaccac
     8641 cgtacccttt cactggcatt ttttctgtta attccactgc aatattattt aatttatatt
     8701 gaatggtatg ctttgttcac cattttaatt cctgtgtacg cctttttact gatgccttgt
     8761 atttcagtgc tggcgcaaga caccgagcat tttcttgaac gtgccgccaa aattcaatgg
     8821 ggcgtgatga ttgctattta ctgcattagc cacgcgccag cgttattgct gttaaagatt
     8881 cccaattatg atgggcacaa taccttgctg ctgttttact ttttattgat cgtacagttg
     8941 agtgatgtgc tgcaatacgt atttggcaat ttgtttggca aaaccaaagt agcgccgatt
     9001 gtaagcccga ataaaacgtt ggaaggcctc attggcggtg gtttaagtgc ggtttgtgtg
     9061 ggcgcgctgc tgtggtggat tacaccgttt acgccactac aaagtgcagg catggcagct
     9121 gtgattgtca tcatgggctt tttgggcggc ttggtgatgt cagctattaa acgtagttta
     9181 ggtgcaaaag attggggcaa catgatagaa ggacacggcg gcatgctcga cagaatggat
     9241 tcagtgagct ttgcggcacc gatttttttc catttagtgc gttacttttt taccgcttga
     9301 attaataaaa ctgtttcaag agtttgatat actagcgcaa cattattttc actggtttta
     9361 aaccatggct gaacaaaacg accacaacaa gcctcacgaa cagcataaac cggatgcaac
     9421 aggtttgcgt aagcgtggca tttatttact gccaaatttg tttacttccg ctgcgctatt
     9481 tgcagggttc tttgcgattg tgcaggcgat gaacagtcac tttgagcttt cagccattgc
     9541 gatatttatc gcgatggtgt tagatggttt ggatggccgc gttgcgcgcc tgaccaatac
     9601 gcaaagtgcc tttggtgcag aatacgatag cttgtctgac atggtatcgt ttggcgtagc
     9661 ccctgcgctc attttatatg tgtgggcatt aaaaccctta ggtaaattag gctggctggc
     9721 ggcctttgct tattgcgcct gcgcggcctt gcgcttggcg agatttaaca ccaagttaga
     9781 cgatgaacat caagacaaac gattctttca aggcttgccc agcccgggtg cggcggcttt
     9841 attggcaggg tttgtatggg tttcatacga atacggcgtg agtggccgtg atatcttttt
     9901 tggcctgtta cagtggaagt ggatggcgtg gttcattacc gtattcgctg gcatttcaat
     9961 ggtgagtgat ttacgttttt acagcggtaa agacattaac ttaaaagaaa gcgttccgtt
    10021 ttttgtgatg ttgggcgtga tgatggcctt tgtcttgatt tcatatcgtc cgccagaagt
    10081 gttgtttttt gtgatggtcg cctacgcgct ttctggctat gtgttgtgga ttcgcgacaa
    10141 actcaagaaa aaagcctaaa tttaagctag ttagcgcttg cagaacccaa taaaaaatac
    10201 tatatttcta tcactatgtt gaacacacat attttcagta tcatttttgc cgttagcttc
    10261 atcggctacg cattatactt acgcaaatta cttgcgttac gtacggctgc gctccgcaat
    10321 accctgcgct agcgcgcaca atattccccc accccctatg taagaatcga cgttgcgcac
    10381 catacgcaac gaaatgccag tatttaagcg aaactcaagc aatagccata gagacttggc
    10441 gcgatacagg ctaaaataaa ctgaataata agtaaggtag ctcaccatga accaaacagc
    10501 agaaaaacaa aatcaaatta ttatttttga taccaccttg cgcgacggag aacaaagccc
    10561 gggcgcggcc atgaccaaag aagaaaaaat ccgtattgcg cgccagcttg aaaaattggg
    10621 cgtgaatgtg attgaagcgg gctttgccgc agctagccct ggcgattttg atgccatatc
    10681 agaaattgca aaaatcatta aaaattccac cgtatgttct ttggcacgcg cgagcgaaaa
    10741 cgatgtgcgc cgcgcgggtg aagcgattaa acatgcggca agcggccgta ttcacacgtt
    10801 tatcgccacc agcaaaatcc acatggaaaa caaattgcgc atgaccgaag accaagtggt
    10861 tgagcgtgct gtgcaagcgg tgaaatgggc caaagagtac accgacgatg tcgagttttc
    10921 ggctgaagac gcggtgcgtt cagagattga ttttttagtg cgtgtatttg atgaggtgat
    10981 tgaagcgggt gctaaaacca tcaacgtgcc tgataccgtt ggctatagca ttccagcccc
    11041 ttggggtgaa cgcatggcga ccttaattaa acgcgtaaaa aatagcgata aagttgtttg
    11101 gagtacacat tgccacaacg atttaggcat ggcggtggcg aactcgttgg ccgcagtaat
    11161 gggcggcgca aggcaagtgg aatgcaccat taacggcttg ggtgaacgtg cgggcaatgc
    11221 cagcttagaa gaagtggtga tggcaataaa aacacgccgc gatattttt
//
LOCUS       DAOQSI010000054        11267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_4959_length_11267_cov_6.259365, whole genome shotgun sequence.
ACCESSION   DAOQSI010000054 DAOQSI010000000
VERSION     DAOQSI010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 11267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11267
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4959_length_11267_cov_6.259365"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(229..1263)
                     /gene="pyrC"
                     /locus_tag="PKL42_05015"
     CDS             complement(229..1263)
                     /gene="pyrC"
                     /locus_tag="PKL42_05015"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNU66468.1"
                     /translation="MTKLLTITRPDDWHLHLRDGAALKAVLPDTARQFARAIVMPNLR
                     PPVTTTELAVAYRQRILDALPEGANFEPLMTLYLTDNTTAEEIATAKASGIIHGVKLY
                     PAGATTNSDSGVTSLDKCATALAAMEKLGVPLLVHAEVTDHDVDVFDRERVFIERNMV
                     PLLKKYPTLKIVFEHITTKDAAAFVTSAPSNVAATITAHHLLMNRNDMFKGGIQPHHY
                     CLPILKREEHRLALVQAATSGNAKFFLGTDSAPHAKHTKEAACGCAGMYTAHTAIELY
                     AEAFEAANALDKLEGFASVYGADFYALPRNSAQITLAKETWIVPANLPFDQDVIVPLR
                     AGQAVHWKLK"
     gene            complement(1276..2118)
                     /gene="rsmI"
                     /locus_tag="PKL42_05020"
     CDS             complement(1276..2118)
                     /gene="rsmI"
                     /locus_tag="PKL42_05020"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNU66469.1"
                     /translation="MAGILYVVATPIGNLGDITLRAIETLKTVDAIAAEDTRHTSGLL
                     SHFGISKKLIAVHEHNEHQLAEKLLLQLQAGENIALVTDAGTPGISDPGAVVVDAVRK
                     AGVKVVPIPGVSAVITALSASGIVQNGFLFHGFLPASGAARRKVLEALTSQTVTLVFY
                     EAPHRIVESIVDMANVLGATRHITIARELTKTFETIHHCMLGEAQQWLASDANQQRGE
                     FVLLVEAAAITEAEALSEDAMRVLKLLLAELPLKQAVKLATDITKEKKNDLYEQALAL
                     KQAN"
     gene            2189..2542
                     /locus_tag="PKL42_05025"
     CDS             2189..2542
                     /locus_tag="PKL42_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNU66470.1"
                     /translation="MKLNKNNAGLEAEKLAATFLAKQGLKLVVQNYHCKYGEIDLIMQ
                     EAKTLVFVEVRQRSNSQFGGAAASITPQKQQKLILTAQHYLQQHGECACRFDAIVMRN
                     ADAQQIEWIRNAFGA"
     gene            2653..3222
                     /locus_tag="PKL42_05030"
     CDS             2653..3222
                     /locus_tag="PKL42_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNU66471.1"
                     /translation="MHNITKLILSTALLSQLVACAPVIVGGAAAGGAMAADRRTSGIY
                     VEDQNIELKALKKVETNLGEEAHVSVTSYNRNVLLTGEVPADESKSKAETLIKEIENV
                     RNITNEIAIGPKSTLSSRSNDTFITSKVKTKFVTENKFPANYVKVVTENSVVYLLGIV
                     TKEEGDAAAEIASNVDGVTRVVKVFEYLP"
     gene            3261..3569
                     /locus_tag="PKL42_05035"
     CDS             3261..3569
                     /locus_tag="PKL42_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HNU66472.1"
                     /translation="MENELVTQNEIIIESITRAGKQFRPSDWVDRMCSSYATFGEDRK
                     LKYSPYLKPKVRNGVRCLAVDLKLKTVNPEGFAQLMHFADENQLNVLDEAGAVIDVPH
                     "
     gene            complement(3772..4248)
                     /locus_tag="PKL42_05040"
     CDS             complement(3772..4248)
                     /locus_tag="PKL42_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNU66473.1"
                     /translation="MQKLFIPALLGFMLYVSAFAPSMAVANHQFVMKDLAGKQHKLSD
                     YRGKWVLVNYWATWCPPCLEEVPDLVSLYHQQKKDVMVFGVVFDYETVASVKSFVDDL
                     RMTYPVVLGDDEVAAQIGSADSLPTTYFYNPKGELVKIKRGLITKEYIENIIKSGK"
     gene            4336..5889
                     /locus_tag="PKL42_05045"
     CDS             4336..5889
                     /locus_tag="PKL42_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66474.1"
                     /translation="MALLLCESIKMALILNLPILKENPVIVAETRPQKISLYLTSLRA
                     QNPQELASYLLSELSTLNRQKVSPTNRIQALDTYRPMLINTCHALAEHYSDAALPLHS
                     QEKAAASAAESLWLELGYGYKLALVDLQNQLFKIGNNTAHCIQHAMHAIAEQALVYYQ
                     TYVTPPSHLWGDLHQLYFCAVQLGVQNQTLLDIDQQTTIENTYVHALLMSLADTQHLS
                     QQNIRLAATFLANHLKDAHISAVAPLESTAGAFVIDLKSNQPPVAFSKRQTFPDPATD
                     ILLLTIDLVRALHLQLSNLQNNQLVSATRENEIDLLTYLIMHWGITPKRLFRRLPKNG
                     DLELVTGIADIQQVSNPNMAINQPEGKQIKPILPSRWQILNISASGMSIRRHPTAEKN
                     IRIGSLVGIKTKNEAQWSLAVVRWANCKSRDKLDIGLELIAPQTDCATASIAAPTADQ
                     QGRNETVLLVPENTATKQTASIIVPRGTYATARQLTLKHKNEIQYALLTKLIDRTQHF
                     EQVQYSVIE"
     gene            6048..6374
                     /gene="trxA"
                     /locus_tag="PKL42_05050"
     CDS             6048..6374
                     /gene="trxA"
                     /locus_tag="PKL42_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HNU66475.1"
                     /translation="MSEHITHLNDAGFEQDVLQSQIPVLVDYWAEWCGPCKMIAPILD
                     DISKEYAGRLKVGKLNIDDNQVTPSKYGIRGIPTLMLFKNGNVEATKVGALSKSQLTA
                     FIDSNI"
     gene            6518..7777
                     /gene="rho"
                     /locus_tag="PKL42_05055"
     CDS             6518..7777
                     /gene="rho"
                     /locus_tag="PKL42_05055"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNU66476.1"
                     /translation="MHLSDLKHLPVTELVEMAIASEIDNASRMRKQDLIFAILKNKAK
                     KGESIFGDGTLEVLQDGFGFLRSPDTSYLAGPDDIYVSPSQIRRFNLHTGDSIQGEIR
                     IPKDGERYFALVKVDLVNGEAPENTKHKILFENLTPLFPTIPLTLERDIKGVENITSR
                     VIDMIAPIGRGQRGLLVASPKSGKTVMMQNIAHAITANHPDVILIVLLIDERPEEVTE
                     MTRSVKGEVVASTFDEPATRHVQVAEMVLEKAKRLVEHKKDVVILLDSITRLARAYNT
                     VIPSSGKVLTGGVDANALQRPKRFFGAARNVEEGGSLTIIATALVDTGSRMDDVIYEE
                     FKGTGNMEIHLDRKMAEKRQYPAINVNKSGTRREELLIEKDVLQKIWVLRKLLYPMDD
                     IEAMEFLLDKIKATKNNADFFDSMRRG"
     gene            7888..8130
                     /locus_tag="PKL42_05060"
     CDS             7888..8130
                     /locus_tag="PKL42_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HNU66477.1"
                     /translation="MKDGIHPNYREVVFQDIGADFSFITRSTIAAKDTIKWTDGKEYP
                     LVKIEVSSKSHPFYTGKQMILDTAGRVDKFRKKYGM"
     gene            8281..9933
                     /locus_tag="PKL42_05065"
     CDS             8281..9933
                     /locus_tag="PKL42_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HNU66478.1"
                     /translation="MRFQLDHDWQGNMASPRARVGEGVKTQLLLILCAIWILFGLIGH
                     APWKPIESTSISIVKNILDTGSFVAPLAVDAANLESPPLYYLSAAVSAKLFSPWLALH
                     DGARLINAVWLSITLLMVGMTGRELWSRGVGRHATFIMIGTIGLVMSAHSLNNEVAHL
                     TSLATGFYALALAKRRPWRASVLFAAALSVGFLAYGLMPLLILLSSAFIMPLLFNTWR
                     SSSFFKFLLAAILLASLPIAGWLLTFQHFHDALFNQWWQSNVTHFGESNHFYFARILL
                     WFAWPALPLALLGLWRYRKQLLTQSKFQLILVFFICSLVFLGIGADSKDIYALPLLLP
                     LVALAAGSVEHLKRGFAAALNWFGVLLFGIIGALIWLGWVAMMTNVPAKIKERLQFLS
                     GLNEISLNWLSLLAAICITVIWAFVCIRAKQTNKSTVTNWAVGMTFSWTLLMTLWLPL
                     LDSAKSYQPVFSSLFHALPTQYHCINSLNVGQSQRLLMHYHTNIKLRPFEDTQELSCD
                     LYLLQDTKGMGKMQPGQEWEMIWSGRRIADRKEGFRLFRRIN"
     gene            complement(10163..10381)
                     /locus_tag="PKL42_05070"
     CDS             complement(10163..10381)
                     /locus_tag="PKL42_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HNU66479.1"
                     /translation="MDIQRLISMANQIGDFYESYPDKSHAEQDIAGHLNKFWALPMRQ
                     QIAKHVQTQNGEGLHVQVKRAIQAHLQL"
     gene            complement(10404..11258)
                     /gene="fdhD"
                     /locus_tag="PKL42_05075"
     CDS             complement(10404..11258)
                     /gene="fdhD"
                     /locus_tag="PKL42_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNU66480.1"
                     /translation="MNRLKLHNGVTQIELESKPFSLLQVEQWHDGLSEEKQDCLAEET
                     PVAIVYNGVSHAVMLATAEDLEDFALGFSLSEEILSDANELYGVEVNIRHNGIELHCE
                     IASERFAQLKERRRTLAGKTGCGLCGAENLAQAMRYPKPISVNHTFASKRILLGLQAI
                     KTQQALQQKTGATHASAYVLADGTIALLREDVGRHNALDKLVGALAKSTLDKTGFILT
                     TSRASFEMVQKTATAGVGMLVAVSAPTGLAVRVAKVCGLALVGFARENRYVVYSHPEL
                     IQHKSIKT"
BASE COUNT         3168 a         2650 c         2390 g         3059 t
ORIGIN      
        1 atgcatttca tttggacgct ctaacattag tgtgacttta tagcggtgca attcacaatt
       61 acggcaactg cttttggtgc tttttatacg cttaaaacct cccgagagaa aagcgctgcc
      121 ctgtgagcct tataaataaa ggctcgcagg gcagcgtaaa aggttgtatc taaaaaccag
      181 ctttaaaaac agaaattgtg atgtgcaaaa tatgcactga tttgaagatt attttaattt
      241 ccaatgcacc gcttgccctg cacgtaacgg cacgatgacg tcttggtcaa aaggtaaatt
      301 ggcaggcact atccaagttt cttttgccag cgtgatttgc gcggaattgc gcggtaatgc
      361 ataaaaatct gccccataaa cactggcaaa cccttctagc ttatctaatg cgtttgccgc
      421 ctcaaatgct tctgcataga gctcaattgc cgtatgtgct gtatacatgc ccgcacaacc
      481 acaagcggcc tctttggtgt gtttggcatg cggcgcgctg tctgtgccta agaaaaattt
      541 agcattgccc gaggttgcgg cttgtaccaa ggccaagcga tgttcttcgc gctttaaaat
      601 tggcaaacaa taatgatgcg gctgaatgcc gcctttaaac atgtcgttac ggttcatcaa
      661 taaatgatgg gcggttatgg ttgcggccac attgctgggc gcgctggtga caaacgctgc
      721 cgcatctttg gtggtgatat gctcaaaaac aattttgagc gtagggtatt ttttcaataa
      781 cggcaccata ttgcgctcaa taaatacgcg ctcgcggtca aacacatcca catcatgatc
      841 tgtcacttct gcatgtacca ataacggcac acctagtttt tccatcgcgg cgagtgccgt
      901 tgcgcattta tctagactgg tgacccctga atcgctattg gttgtggcgc ctgcagggta
      961 gagttttacc ccgtgaataa tgccgctcgc cttagctgtt gcgatttctt cagccgtggt
     1021 gttatcggtg agatacaagg tcatgagcgg ctcaaaatta gcgccttccg gtaatgcatc
     1081 taaaattcgt tggcgataag caacggctaa ttctgtggtg gtgactggcg ggcgcaaatt
     1141 cggcatcaca atggcgcggg cgaattgcct ggcggtgtca ggcaacacgg ctttaagcgc
     1201 cgcgccgtca cgcaagtgta agtgccagtc atctgggcgg gtaatggtaa gtagttttgt
     1261 catcttattg gctttttaat tggcttgctt gagggcaagc gcttgttcat ataaatcatt
     1321 tttcttttct ttagtaatat cagtcgccaa cttgacggct tgttttaatg gcagttctgc
     1381 tagcaataat tttaaaactc tcatagcatc ttcagacagc gcttcagctt ctgttatggc
     1441 agccgcttca accagtaaca caaattcacc acgctgctga ttagcatcac ttgctaacca
     1501 ctgttgcgct tcgccaagca tgcaatggtg aatagtttca aacgttttgg tgagctcacg
     1561 cgcaatggtg atgtgtcgcg tagcacccag cacgttggcc atgtcaacaa tgctctcaac
     1621 aatacgatgc ggggcttcat aaaacaccaa tgtaaccgtt tgagatgtga gtgcttcaag
     1681 cactttacgc cgcgctgcgc cgctggccgg taaaaaccca tgaaataaaa agccattttg
     1741 cacaatgccg gatgctgaca acgcagtaat caccgcactc acacccggaa tgggcactac
     1801 cttaactccc gctttgcgca cggcatccac caccacggca cccgggtcac taatgccagg
     1861 cgtaccggca tcggtcacca atgcaatatt ttcaccggct tgcagttgca ataatagttt
     1921 ttcagccaat tgatgctcgt tatgctcatg cacagcaatc agttttttgc tgatgccaaa
     1981 atgcgataac aagccagacg tgtggcgcgt gtcttctgcg gcaattgcat ctaccgtttt
     2041 aagcgtttca atggcgcgta aggttatatc gcctaagttg ccaatgggcg ttgcaaccac
     2101 atataaaatg cccgccatca aaagcacccg ctatttaatt taaattgaat cattttataa
     2161 gtttaccgct ttaggtaaac tctcgcctat gaaattaaac aaaaacaacg cgggcttaga
     2221 agctgaaaag ttagctgcaa cttttttagc aaaacaaggc ctcaagcttg tggtacaaaa
     2281 ctatcattgc aaatatggcg aaatcgactt gattatgcaa gaggcaaaaa cccttgtatt
     2341 tgttgaagta agacaacgtt caaacagcca atttggcggc gctgcggcca gtattacccc
     2401 gcaaaaacaa caaaaactca ttcttaccgc acaacattat ctacaacaac atggcgagtg
     2461 cgcctgccga tttgatgcga ttgtcatgcg aaacgcagat gcccagcaga ttgaatggat
     2521 tcgcaatgct tttggcgcct aattggcgta tgatgcgcac aaaatgacaa attgttatat
     2581 tttattaaca atttaatggt tcaatagccc ctaagatttt ggcaatacaa catccactta
     2641 aaggagtttt agatgcataa catcaccaaa ctaattttat ccaccgcatt attaagccag
     2701 ttggttgcgt gtgcgccagt gattgtcggt ggcgcagccg ccggcggtgc aatggctgcc
     2761 gatagacgca cttccggcat ttatgtagaa gaccaaaata ttgagcttaa agcccttaag
     2821 aaggttgaaa ccaatcttgg tgaagaagca catgttagcg tgaccagcta taaccgcaac
     2881 gtgctactca caggtgaagt gcctgctgat gaatcaaaat ccaaagcaga aaccttaatc
     2941 aaagaaatcg aaaacgtccg caatattact aacgaaattg ccatagggcc aaagtccacc
     3001 cttagttcac gtagcaacga cacctttatc acctcaaaag taaaaaccaa atttgtcacc
     3061 gaaaataaat tccctgcgaa ttatgtcaaa gtggtcactg agaacagcgt ggtttattta
     3121 ctaggcattg tcaccaaaga agaaggcgat gcggctgcgg aaatagccag caatgtggat
     3181 ggcgtcacac gcgtggttaa agtttttgaa tatctgccgt aattattaac acacacatca
     3241 acttcatggg taaacctgtc atggaaaacg aattagtcac acaaaatgaa attattattg
     3301 aaagcatcac gcgcgcgggc aaacagtttc gcccaagtga ctgggtggat cgtatgtgca
     3361 gcagctacgc cacctttggt gaggaccgca aattaaaata ttcgccttat ttaaaaccaa
     3421 aagtacgcaa tggcgtgcgt tgcttggctg ttgatttaaa attaaaaacc gtcaaccctg
     3481 aaggctttgc gcagctcatg cactttgctg atgaaaacca actgaatgtg ttggatgaag
     3541 cgggtgctgt catcgacgtg ccgcattaag gtttcagttt aagtttaggt tttgttcaaa
     3601 caagggcgct atttgcgccc ttaatttttt actgaaatta acgggaatat aaacaatttt
     3661 caccctgaaa aaacgccgag cgaaaaacat tgccctgcga gcctttattt ataaggctcg
     3721 cagggcatag aaaaaggttg catcaaaaat aaagcaaaat atcttaaaaa atcacttgcc
     3781 tgatttgatg atattttcaa tatattcctt ggtaattaag ccgcgcttaa ttttcaccag
     3841 ttcgcctttt ggattataaa aataagtggt tggcaacgag tcagcactgc cgatttgcgc
     3901 cgcaacttca tcatccccca gcacaactgg gtatgtcatg cgcagatcat ccacaaaact
     3961 cttcaccgac gccacggttt cataatcaaa caccacgccg aacaccatca cgtctttctt
     4021 ttgctgatgg tagaggctca ccaaatcagg cacttcttcc aaacatggcg ggcaccatgt
     4081 ggcccaataa ttcaccagca cccattttcc ccgatagtca gagagtttgt gctgcttgcc
     4141 tgcgaggtct ttcatgacaa attgatgatt cgccaccgcc atagacggcg caaatgcgct
     4201 gacatacagc atgaacccta gcaatgctgg gatgaataat ttttgcatat taactttctc
     4261 tcggttctca atcaaacatt tgctcaggcg tttatcaata tcaactagtc taaatatcaa
     4321 cattatcata caatcatggc attgcttttg tgtgaatcca taaaaatggc gttaattctt
     4381 aatctaccga ttcttaaaga aaaccctgtg attgttgcgg aaacacgtcc gcaaaaaata
     4441 agtctttatc tgacaagttt acgcgcgcaa aatccgcagg aattggcttc ttatttgcta
     4501 agtgaactca gcacattgaa tcggcaaaag gtttcgccaa ccaatcgaat tcaggcatta
     4561 gacacttatc gtccaatgct tatcaacacc tgccacgcgc tagccgagca ttatagcgac
     4621 gctgcgctac cactacatag ccaagaaaaa gccgctgcat cagctgctga gtcattatgg
     4681 ctagagttag gctacggcta taaactggca ttagtcgatt tgcaaaatca gctctttaaa
     4741 attggcaaca atacagcgca ttgtattcag cacgccatgc acgccattgc tgaacaggcg
     4801 ctggtgtatt accaaacata tgtcaccccg cccagccacc tttggggcga cttgcaccag
     4861 ctttattttt gcgcagttca gcttggggtg caaaaccaaa ccctactaga tattgaccaa
     4921 caaaccacta ttgaaaatac ctatgtacat gctttgctga tgtcgcttgc cgatacacag
     4981 cacctgtcac agcaaaacat caggcttgcg gcaacttttc tagccaacca cttaaaggat
     5041 gcccacatct ctgcagttgc cccgctagaa tctactgccg gcgcgtttgt cattgatttg
     5101 aaatcaaatc agccgccagt ggccttcagt aagcggcaaa cgttccccga ccccgcaacc
     5161 gacatcttat tgctgacaat tgatttggtg cgcgcattgc acttgcaact aagcaatttg
     5221 caaaacaatc aactggtgag cgccacccgt gaaaatgaaa ttgatttgct cacctacctg
     5281 attatgcact ggggaatcac ccctaaacgt ctgttcaggc gtttgccaaa aaatggtgat
     5341 ttagaattag tgactggcat tgccgatata caacaagtat ctaaccccaa catggccatc
     5401 aaccagccag agggcaaaca aatcaaaccg attttgcctt ctcgctggca aatcttaaac
     5461 atcagtgcca gcggcatgtc gattcgccgc catccaactg cagaaaaaaa catcagaatc
     5521 ggcagcctgg tcggcattaa aactaaaaat gaagcgcaat ggtcacttgc tgtagtacgt
     5581 tgggccaatt gcaaaagtcg cgacaaatta gacattggcc ttgagctgat tgcaccgcaa
     5641 accgattgcg ccaccgcgag cattgcagcg ccaactgctg atcaacaagg tcgtaacgaa
     5701 accgttttat tggtaccaga aaatactgca accaaacaaa cggcgagcat cattgtgcct
     5761 agaggcactt acgcaacagc acgccaactg accttaaaac acaaaaatga aattcaatat
     5821 gcgctattaa caaaattaat tgaccgaaca cagcattttg aacaagtgca atacagcgtg
     5881 attgaataac attgaaacta tctttattga acgctagccc tgcaaaatgc cgttattatc
     5941 aataaatacc aattaaaaaa ataaagattt aacgcttcac taaagctggt tttttcgtta
     6001 cgatacatct ttactataaa agcattattt aattttcggg gtttaccatg agcgagcata
     6061 ttacacacct aaatgacgca ggatttgagc aagacgttct gcaatcacag attcctgttt
     6121 tagtagatta ctgggcagaa tggtgcggcc catgcaaaat gattgcacca attttagatg
     6181 acatttctaa agaatatgca ggccgtctaa aagtgggcaa actcaacatt gatgacaacc
     6241 aagtcacgcc atctaaatac ggcattcgtg gcattccaac cttaatgtta tttaaaaatg
     6301 gcaatgtgga agccacaaaa gtaggcgcgt tatcaaagtc tcaattaacc gcttttattg
     6361 acagtaacat ctaaacgcat tacgctacgc atatattttc agtgcttggt tgtcttttac
     6421 ttgcaattag tgaattaaac caagcacctt agtacccacc tttattttaa tcagtaactt
     6481 ctaacatccc acaccactct tgctttagtg agccttcatg cacttatcag accttaaaca
     6541 cctacctgtc accgaattag tagaaatggc catcgccagc gaaatcgaca atgcgagccg
     6601 tatgcgcaag caagatttga tttttgccat tttaaaaaac aaggctaaaa aaggcgaaag
     6661 tatttttggc gatggcacgc tagaagtgtt gcaagatggc tttggttttt tgcgctcccc
     6721 agatacctca tatttggcag ggcctgacga tatttacgtt tcaccttcac aaattcgacg
     6781 ttttaatctg catacaggcg acagcatcca aggtgaaatt cgtattccca aagatggcga
     6841 acgttatttc gcactggtca aagtagattt agtgaatggt gaagcgccag aaaataccaa
     6901 gcataaaatt ctgtttgaga atttaactcc gctttttcct accattccac tcacgttgga
     6961 gcgcgacatc aaaggtgtgg aaaacatcac cagccgtgtg attgacatga ttgcgccaat
     7021 tggccgtggg cagcgcggcc tgttagtcgc cagcccaaaa agcggtaaaa ccgtgatgat
     7081 gcaaaatatc gcgcatgcga ttaccgccaa tcatccagac gtgattttaa ttgtgctgtt
     7141 gattgatgaa cgccctgaag aagtaacgga aatgacacgt tcagtcaaag gcgaggtcgt
     7201 tgcttcaacg tttgacgaac ccgcaacacg ccacgtgcaa gtcgctgaaa tggtacttga
     7261 aaaggccaag cgtttggtcg aacataaaaa agacgtggta attttactag actccattac
     7321 gcgtctagcg cgtgcttaca atacggtgat tccttcctca ggcaaagtgc ttacaggcgg
     7381 cgtagatgct aacgcgctgc agcgcccaaa acgctttttt ggtgccgcac gtaacgtaga
     7441 agaaggcggc tcactgacga ttattgctac ggccctagtt gatacgggtt ctcgcatgga
     7501 tgatgtcatc tatgaggagt ttaaagggac gggcaatatg gaaatccatc ttgaccgtaa
     7561 aatggcggaa aaacgtcaat atcctgcaat taacgtcaat aaatccggca cgcgtcgcga
     7621 agaattactc attgagaaag atgtgctgca gaaaatttgg gtactacgca aactgctcta
     7681 cccaatggac gatatcgagg caatggaatt cttgttggac aaaatcaagg ccaccaaaaa
     7741 caatgcggat ttctttgaca gcatgcgtcg cggctaaagc gacttaatta ttcgcttgac
     7801 cttgcttcat tacttaaaaa tcaatataat acgcggctaa tttatttaaa cgtgccgtat
     7861 cgttcggcaa acatagaggc ttacaaaatg aaagatggaa ttcacccaaa ttatcgcgaa
     7921 gttgtttttc aagacatcgg cgctgatttt agctttataa cacgctcaac cattgcggca
     7981 aaagacacga ttaaatggac tgacggtaaa gaatatccac tcgtgaaaat tgaagtttca
     8041 tcaaaatcac accctttcta tactggcaaa caaatgattc ttgatacggc tggtcgtgtg
     8101 gataaattcc gtaaaaaata cggtatgtaa tctaacttgc aacaatggtt gcaatattaa
     8161 ggcagcttac gctgcctttt tttattttta caattgcgat tcccatatct ggctatggct
     8221 tcagtctgca atcagttaaa atcaactatt aactactctt tcatcaatta agctttattc
     8281 atgcgctttc aattagatca cgactggcaa ggcaacatgg catctccccg cgcgcgcgtg
     8341 ggggaagggg tgaaaacgca gttattgcta attttgtgcg ccatttggat actatttggt
     8401 ttaattggcc atgcaccctg gaaaccaata gaatcaacca gcatcagcat cgtcaaaaac
     8461 attctagaca caggcagctt tgtggcaccg ttggcagtag atgccgccaa ccttgaatct
     8521 ccgccacttt attatttatc cgcagctgtt agcgccaagc tattctcgcc atggttagcg
     8581 ctacatgacg gcgcaaggct catcaatgcc gtttggcttt ctatcacgtt actgatggtg
     8641 ggaatgacag gtcgagaatt atggtcgcga ggagtgggcc gccatgcgac atttattatg
     8701 atcggcacca tcgggctggt catgagtgcg cacagtttaa ataatgaagt tgcacacctg
     8761 accagccttg ccacaggctt ttatgcgctc gcactggcaa aaaggcgccc ttggcgtgca
     8821 agtgtgctgt ttgcggccgc cttaagtgtt ggctttctgg cttacgggct gatgccatta
     8881 ttgattttac tcagttcggc cttcattatg ccgttgttat tcaatacttg gcgaagtagc
     8941 agttttttta aatttcttct cgccgccatt ttactggcaa gcttgcctat cgctggctgg
     9001 ttacttactt ttcagcactt tcatgatgcg ttattcaacc agtggtggca atcaaacgtc
     9061 acgcactttg gcgaatcaaa ccatttctat tttgcgcgaa ttttgctgtg gtttgcatgg
     9121 ccagctctgc ctttggcgct actaggatta tggcgctatc gcaaacaatt gctcacgcag
     9181 tcgaaatttc aactgatact ggtgtttttt atctgtagcc tggtatttct tggcataggt
     9241 gcggattcta aagacattta tgcacttccc ctgctgctgc cacttgtggc gctggcggct
     9301 ggcagtgttg aacaccttaa aagagggttt gcagccgcgc taaactggtt tggcgtatta
     9361 ctatttggca tcataggcgc gctcatttgg ctaggctggg ttgccatgat gaccaatgtg
     9421 ccggccaaaa ttaaagaacg cctgcaattt ttatctggac tcaacgagat tagcttaaac
     9481 tggctatcct tgttagcagc catctgcatc actgtaatct gggcgtttgt gtgtattcgc
     9541 gccaaacaaa ccaataagtc caccgtcacc aactgggccg ttggcatgac ttttagctgg
     9601 acattgctaa tgaccctttg gctaccgctc cttgattcgg ctaaaagcta tcaacctgtt
     9661 ttctccagtt tgtttcacgc cttaccaact cagtatcatt gcatcaatag cctgaatgta
     9721 ggccagtcac agcgcctgtt aatgcactat cacaccaaca tcaaactgcg cccgtttgaa
     9781 gatacgcaag aactcagttg tgatttgtat ttgctgcaag acaccaaagg catgggcaaa
     9841 atgcagcccg gtcaagagtg ggaaatgatt tggagtggca gaagaatcgc cgatagaaaa
     9901 gaaggattca ggctatttcg gcgcatcaat taacaggcaa atgagtacta aaacaccact
     9961 tgattgaagc tgaataatag tcgaaattct acgagaaaaa atttacgccc tggaagcctt
    10021 tgtttataag gctcgcagag catcaaaaaa ggttgaatgt taaattgcct tgaaaaatag
    10081 tcttttagac cctgttaatt tagcgccaag ctatccgtct aatggcaaga gtaactgctt
    10141 gctcaccgta ggtgaatttt tattaaagtt gcaagtgtgc ttgaattgca cgctttactt
    10201 gtacgtgtaa accttcacca ttttgtgttt gaacatgttt cgcaatttgc tggcgcattg
    10261 gcaatgccca aaacttgttt aaatgcccgg caatatcttg ctcggcatga cttttatcgg
    10321 gataagactc ataaaaatcg ccaatttgat ttgccattga aattaaacgc tgtatatcca
    10381 taattgtatt cctgtaagcc tagtcaagtt tttattgatt tgtgttgtat cagttctggg
    10441 tgactataaa ccacgtaccg attttcacgg gcaaaaccta ccagcgctaa tccacatact
    10501 tttgcaacgc gcaccgccaa ccctgtgggc gcggacacag cgaccaacat accaacaccc
    10561 gcagtggcag ttttttgcac catttcaaaa ctggcgcggc ttgtggtaag aataaacccc
    10621 gtcttatcta gcgtcgattt ggcaagtgcg ccaaccaatt tatcaagtgc gttatgccgc
    10681 ccgacgtctt cgcgcaatag ggcgatggtt ccatccgcta acacataagc actggcgtgc
    10741 gtagcacctg tcttttgctg caaggcctgt tgtgttttaa tggcttgcaa ccctagcaat
    10801 atacgctttg atgcaaatgt atggttaacc gagattggtt tcggatatcg cattgcttgt
    10861 gctaaatttt ccgcaccgca cagcccgcaa cctgttttgc ctgccagcgt acggcggcgc
    10921 tctttcaatt gtgcaaaacg ttcactggca atttcacaat gcagttctat gccattgtgc
    10981 cgtatattga cctcaacccc gtatagctca ttcgcatcac ttaaaatctc ttcagatagc
    11041 gagaaaccta acgcaaaatc ctctaaatct tcagcggttg ccagcataac cgcatgcgaa
    11101 actccattat aaacaatcgc gaccggcgtt tcttctgcta aacaatcctg tttttcttct
    11161 gaaagaccgt catgccactg ttcgacctgt agcaggctaa atggcttact ttcaagttct
    11221 atctgcgtga ctccattatg caactttagt cgattcattt gcacgtg
//
LOCUS       DAOQSI010000055        11238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_4979_length_11238_cov_5.357596, whole genome shotgun sequence.
ACCESSION   DAOQSI010000055 DAOQSI010000000
VERSION     DAOQSI010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 11238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11238
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4979_length_11238_cov_5.357596"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..784)
                     /locus_tag="PKL42_05080"
     CDS             complement(257..784)
                     /locus_tag="PKL42_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HNU66481.1"
                     /translation="MSQPLVIDNLAFSKKNEALSGVLLLTDCPRLQELLHQPSLKAES
                     NAHEIVFDLKGTTNSLNQHFLSLSITAKLSANCQRCLSAMPLTIKLHFNYLISDVDDA
                     ALEALELDNVDDFDLLQANANMDLAALIEDEVIMALPIAPTHENACVELVTQSGEKSN
                     PFAALKGLIKPQTGE"
     gene            927..2345
                     /locus_tag="PKL42_05085"
     CDS             927..2345
                     /locus_tag="PKL42_05085"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNU66482.1"
                     /translation="MFKKILIANRGEIAVRIVRACSEMGIKSVAIYADADRQALHVKK
                     ADEAYNIGADPVAGYLNAHNIVNLAVASGCDALHPGYGFLSENPELAEICARRGIKFI
                     GPNASVIRQMGDKIQARNAMTAAGIPCTPGSDGNLENLDQAVALAKKIGYPVMLKATN
                     GGGGRGIRRCDSEKELLSNYDRVISEASKAFGKPEVFLEKCVVSPRHIEVQVLADSQG
                     NVIHLFERDCSIQRRNQKLIEIAPSPQLTQAQREYIGGLAVKAAKAVGYENAGTVEFL
                     LDSDNTFYFMEMNTRLQVEHTVTETITGIDIVQEQIRVAYGLPLQYVQKEVQFRGYAM
                     EFRINAEDPKNDFLPSFGKITRYYSPGGPGVRMDAAIYTGYVIPPYYDSMCAKLTVWA
                     LDWESVIERGRRALDDMLIYGVKTTIPYYQQILKHPDFRNAEFNTGFVETHPELTHYA
                     NEIAPEVIAAAISAAIAAHEGV"
     gene            2403..4274
                     /gene="oadA"
                     /locus_tag="PKL42_05090"
     CDS             2403..4274
                     /gene="oadA"
                     /locus_tag="PKL42_05090"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HNU66483.1"
                     /translation="MTNNQATKSSKVHITELVLRDGHQSLIATRMRTEDMLPICAKLD
                     AIDFWSLEAWGGATFDACVRFLKEDPWERLTKLRKALPNSKIQMLLRGQNLLGYRHYS
                     DDVVQAFVKKSADNGVDVFRIFDAMNDMRNLRTSVEAVKKVGKIAEGAISYTTSPVHD
                     VAHFVGLAKELEGMGCDTIAIKDMAGLLTPQSTAELVKALRTAVSLPIHLHSHATSGL
                     SAMCFLKGIEAGATIVDTSNSSFGEGASHSSTESIVAALQGTEYDTGINLQALQEITA
                     YFREVRKKYWQFESDFTGVDTRVLVNQVPGGMISNLSNQLKEQGALNRMDEVLAEIPR
                     VREDLGFPPLVTPTSQIVGTQAVLNVMTGGRYKSVTNEVKNYLLGHYGKSPAAVNQTV
                     KQNAVGNAEVITCRPADLLTPEMAKLTSEADRFAKTEEDVLTYAMFPDIGKTYLQERN
                     AGSLQPEALLDKETVNASASRFAPSEFKVTLHGETFHINLTGSGHPGEEKRPFYVSID
                     GIAEEVIVETLSEIEVTSGKTSGNNKKKAATSKASNGRPRPSHAGHVTTSMPGTIVAV
                     KAKVGDKVTAGDGVLVIEAMKMENEIQASTTGIVVAVHVVKGDTVTPDEALLEIQPE"
     gene            4344..4961
                     /locus_tag="PKL42_05095"
     CDS             4344..4961
                     /locus_tag="PKL42_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HNU66484.1"
                     /translation="MKQSVKSSAKLKSKQTLILASSSEFRRALLQKLQIPFSSISPHV
                     DETSLEGEKPHETALRLAKEKAKKIGQEYPHALIIGCDQVATLDGEQIGKPGNHSNAT
                     KQLQRMRGREVTFHSALCLYNAATGNMQAEVVPYLVRFRQLSDEQIENYLNKEQPYHC
                     AGSAKSEGLGIALIERMLGEDPNALIGLPLIKLINMLQVEDVQVI"
     gene            5091..6578
                     /locus_tag="PKL42_05100"
     CDS             5091..6578
                     /locus_tag="PKL42_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HNU66485.1"
                     /translation="MPLKYKLIVIATLSTLMTGVVAMEKNQKNSDNTPSSLDETRITQ
                     DDQKTVAVTIYNQDLALVKDTRKVNLKKGLNALALRDVSAQIRPETALLRSINAPGSL
                     TLLEQNFDFDLLTPEKLLEKYVGKTVSIVKTHPTTGAETSEKATVLSANNGVVLKIGN
                     RIETGVSGRIVYDDVPANLRDRPTLVTQVDNSGATDQTMELSYLTGGLGWKADYVAEL
                     SPKEDSIDLSGWVTLTNTSGATYKNAKLQLVAGDVNRVQPEYPRAAMMRKNADMVMEA
                     AASAPMAEESLLEYHLYTLDRPTTIAESQTKQVALLSASNIPARKELVLKGAEYYYTA
                     QYGEIGTKLKVSTFVEFENKEASKLGMPLPKGVLRVYKKDKAGNAQFVGEDNIDHTPK
                     NETVRIKLGESFDVTADKKQTEFKVLPNPQKGRSAYESAFEFTLKNAKKEKMTVLVQE
                     PIGGDWKIINENHPHQKANSHLAIWKIDVPAEGKTTLTYRVQTKY"
     gene            6878..7603
                     /locus_tag="PKL42_05105"
     CDS             6878..7603
                     /locus_tag="PKL42_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNU66486.1"
                     /translation="MSNAPVKLGTLYFIPVTLGDDNFHKVLPPEVVAIAQKLDTFIVE
                     NEKTARHFLSTIKTAKPVRELTLLTLNEHTEDKQIPALLAPLLAGIDVGLMSEAGCPG
                     IADPGAKLAALAHPKGIKVAPLVGPSSILLSLMASGLNGQRFTFLGYIPQDKAARINR
                     LKEIEKQSAKNNETQIFIETPYRNQHLLEDILAHCQANTRLCVACNISLESEVIVTKT
                     IAQWKKSPLPDLHKKPTVFLLMA"
     gene            complement(7600..8106)
                     /locus_tag="PKL42_05110"
     CDS             complement(7600..8106)
                     /locus_tag="PKL42_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66487.1"
                     /translation="MRKATYLIWLLIFLAGFYIAGLEFFPKSASQATSANMAYMGVAA
                     ALTIFLMIGLFPLVTLAYLLNWKDLFNQTGTVSAWRYFEAIIFVIPALLVGYLWVENT
                     WAINQLITMLAAVSALPIILFGVVNVTQYWRSKEYKTLRRNVELAYFVVPALLMVAYL
                     WVSFIKAG"
     gene            complement(8103..8708)
                     /gene="rnhB"
                     /locus_tag="PKL42_05115"
     CDS             complement(8103..8708)
                     /gene="rnhB"
                     /locus_tag="PKL42_05115"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNU66488.1"
                     /translation="MQISKIICGVDEAGRGPLAGSVYAAAVILDANQPIIGLADSKKL
                     SEKRRDALAIEIKQKALAWAIASCTAQEIDEINILQASLLAMKRAIEDLQAQLKLTTE
                     MGEIFVQVDGNKCPNISLPCEAIVKGDSKVQAISAASILAKTARDAELYELDKIYPQY
                     GFAQHKGYPTAAHLAALAEHGICVEHRLSYAPVKKIVRGLV"
     gene            complement(8940..10109)
                     /gene="lpxB"
                     /locus_tag="PKL42_05120"
     CDS             complement(8940..10109)
                     /gene="lpxB"
                     /locus_tag="PKL42_05120"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNU66489.1"
                     /translation="MVMKIAIVAGEASGDLLGSHLMAALKKHHPHIEFIGIAGPKMIG
                     EGAKSLFSIEQLSVRGYVEVIKHLPGLLKLRRALFQQLLATKPDLFIGVDAPDFNFWL
                     EKKLKNKGIPAIHYVSPSIWAWRKGRIGGIKKAVSHILALFPFEPAIYQKAGIPVTYV
                     GHPLADILPLVPDIIGARENLKIRTTQTVIAMLPGSRQGEVEQHAELFIQAAHRINQS
                     LIEEGKHDTVFLVPLITRETRAIFKKKLHQFLVSLPESSPSPPITVMFGHAHDAMEAA
                     DLIIVASGTATLEAALLKKPMIITYRMSNLSWQILKRMRLQPYVGLPNILAERFVVPE
                     LLQQDATPEKIAETAHKLLSDKALQTDIKEEFTRMHHQLRQNTAEKAAEAILKFL"
     gene            complement(10114..10893)
                     /gene="lpxA"
                     /locus_tag="PKL42_05125"
     CDS             complement(10114..10893)
                     /gene="lpxA"
                     /locus_tag="PKL42_05125"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNU66490.1"
                     /translation="MSAKIHATAIIDKNAELDSSVEVGPFSIIGPNVKIDAGTRIAGH
                     VTISDHTIIGKHNQIFQYSSLGEAPQDKKYKNEPTLLEIGDNNTIREFCTFNRGTVQD
                     KGTTKIGNDNWIMAYVHLAHDCVIGNHTIFANNASLAGHVDVHDYAILGGFTLIHQFC
                     KIGSHVITAVGSVVFKDIPPYVTAAGYDAKPHGINAEGLKRRGFSSEAILEVKRAYKT
                     LYRNGLTLEEAKLELAEKQKSCQEIGLLLDFLNTSTRGIVR"
     gene            complement(10938..>11238)
                     /gene="fabZ"
                     /locus_tag="PKL42_05130"
     CDS             complement(10938..>11238)
                     /gene="fabZ"
                     /locus_tag="PKL42_05130"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNU66491.1"
                     /translation="FPYHPVMPGVLIVEAMAQAAAVLSFKTMDAKPNDESVYYFAGID
                     SARFKKPVSPGDQIVLNVKIDRILKGIWKYTGSASVDNEVVAEASMMCILKAIEK"
BASE COUNT         3334 a         2473 c         2443 g         2988 t
ORIGIN      
        1 ttcgcgtatt gctaaaaacg gatgatattc cacataaaat tttattcaaa aactgccatt
       61 tttgaatagt aaaagtacgc catcattccg ccgagaaaaa ttatcgcctc tgcaagcctt
      121 tatttataag gcttccagag catcgaaaaa ggttgtgatt aaaaatggcc tatttgaagc
      181 aaaatttcaa tcattaacga gacgtgaaaa gcctgtttaa aattcaggtg aatttagcaa
      241 aaaaaccctg attttatcat tcaccggttt gtggttttat cagtcctttt aacgcagcaa
      301 acgggttgga tttttcgcca ctttgggtga ccaattcaac acaagcattt tcatgcgtgg
      361 gcgcaatggg cagcgccatg atgacttcat cttcaataag tgcggctaaa tccatattgg
      421 cgttggcttg cagcaaatca aaatcatcca cgttatcaag ttccagcgcc tctaaggctg
      481 catcatcaac atcgctaatc aggtaattaa aatgcagttt tatagtgagc ggcattgcgc
      541 tcaagcagcg ctggcaattt gcgctgagtt tggcggtaat gctgaggctt aaaaaatgtt
      601 ggttgagcga attggtcgtg ccttttaaat caaatacaat ctcgtgcgcg ttggattcgg
      661 cttttaatga tggttggtgc aataactctt gcagccttgg gcagtctgta agtagcagaa
      721 cgccgctaag cgcctcattt ttttttgaaa aagctaaatt atcaatcact aaaggttggc
      781 tcattatatt tttaactcgt catcagggtg gtttaatctt aaagtttatg cgtaaattta
      841 acacgggaag cctgagttta gtatttagct accggctcgt gcatgttaca attgccgagt
      901 tttagtaata actttaaggc tcgtttgtgt ttaaaaaaat tctaattgct aaccgtggtg
      961 aaatcgcagt gcgcattgta cgcgcttgct ctgaaatggg catcaaatct gtggcaattt
     1021 atgccgatgc agatcgtcag gctttgcatg ttaaaaaagc agatgaagcc tataacatcg
     1081 gcgcagaccc agtagcaggc tacttaaatg cacataatat tgtgaatttg gccgtggctt
     1141 ctggctgtga cgccctgcac cctggctatg gtttcttgtc tgaaaaccct gagttagctg
     1201 agatttgcgc acgccgtggc attaaattta ttgggccaaa tgcgtctgta attcgccaaa
     1261 tgggcgacaa aattcaagcc agaaacgcca tgaccgctgc tggcattccc tgcacaccag
     1321 ggagtgatgg caatttggaa aatctggatc aagccgttgc gctagcaaaa aaaattggtt
     1381 acccagtcat gcttaaggcc accaatggcg gtggcggtcg cggtattcgc cgttgtgata
     1441 gtgaaaaaga attattaagc aactacgacc gcgtgattag tgaagccagc aaggcgtttg
     1501 gtaaacctga agtgttttta gaaaaatgtg tggtttcacc acgccatatt gaagtgcaag
     1561 tgctggccga ttcacaaggc aatgtgattc atctatttga gcgcgactgc tcaattcaac
     1621 gccgcaacca aaaactgatt gaaattgccc cctccccgca actcacgcag gcacaacgtg
     1681 aatatattgg aggcttagcg gtaaaagcgg ctaaagcagt aggctacgaa aatgctggta
     1741 ctgtggagtt tctgctggat tcagacaaca cattctattt tatggaaatg aatacgcgtc
     1801 tacaagtaga gcatactgtc actgaaacca tcaccggcat tgatattgtg caagagcaaa
     1861 ttcgcgtcgc atacggctta cctttgcaat atgttcaaaa agaagtgcaa ttcaggggct
     1921 atgcaatgga gtttcgcatc aatgcggaag accctaaaaa cgactttttg ccaagctttg
     1981 gcaaaataac tcgctattat tcacctggtg ggcccggcgt gcgaatggat gctgccattt
     2041 atactggtta cgtgattccg ccctattacg attcaatgtg tgccaagctc accgtatggg
     2101 cactggactg ggaaagtgtg attgagcgtg gacgccgcgc attagatgac atgctcattt
     2161 acggtgttaa aacaaccatt ccttactatc agcaaatttt gaagcaccct gattttagaa
     2221 atgcagaatt taataccggc tttgtagaaa ctcaccctga gctcacgcac tatgcgaatg
     2281 aaattgcacc agaggtcatt gctgccgcca tttctgctgc cattgctgca catgagggtg
     2341 tgtagattaa atataataaa aacatataat taaacatatt atttaaagta aagagttaag
     2401 ctatgacaaa caaccaagcg actaaatctt ccaaagtaca cattactgaa ctggtgttgc
     2461 gtgacgggca tcagtcgtta attgcaacac gcatgcgtac agaagatatg ttgccaatct
     2521 gtgcaaaatt ggatgcgatt gatttttggt cgctcgaagc ttggggcggc gctacgtttg
     2581 atgcctgtgt acgcttttta aaagaagacc cgtgggagcg gttaactaaa ttacgtaagg
     2641 ctttgcctaa cagcaaaatt caaatgttgc tgcgcggcca gaatttgctt ggctaccgcc
     2701 attattctga cgatgtcgtt caggcatttg tcaaaaaatc agcggataac ggtgttgacg
     2761 ttttccgtat ttttgacgca atgaacgata tgcgtaactt aagaacttca gttgaagctg
     2821 tcaaaaaagt aggcaaaatt gctgaaggtg ctattagcta tacaactagc cctgtacacg
     2881 atgttgcgca ttttgtcggg cttgccaaag aactggaagg catgggctgt gacaccattg
     2941 ccattaaaga tatggcgggc ttgctcaccc cacaaagcac ggctgagctt gtgaaagcat
     3001 tacgcacggc agtgagctta cccatccatt tacatagtca tgccacttca ggcttgtcgg
     3061 caatgtgctt ccttaaaggc attgaagctg gtgccaccat tgtagatacc agcaactctt
     3121 cttttggcga aggtgccagt cattcttcta ctgaaagtat tgtggcagcg ctacaaggca
     3181 ctgagtacga taccggcatc aacctgcagg cattacaaga aattactgct tatttccgtg
     3241 aagtgcgtaa aaaatactgg caatttgaaa gcgatttcac gggcgttgat acgcgtgtat
     3301 tggtcaacca agtgccaggt ggcatgattt ccaacttatc taaccaatta aaagaacaag
     3361 gcgcactgaa ccgcatggat gaggtgcttg ctgagattcc gcgtgttcga gaagacttag
     3421 gctttccgcc gctcgtcacg ccaacttcgc aaattgtggg cacacaagca gtgctgaacg
     3481 taatgacagg tggacgatac aaatctgtca ccaatgaagt taaaaactat ttgctcggcc
     3541 attacggaaa atcaccagcc gcagtcaatc aaactgtcaa acagaatgcc gttggaaatg
     3601 ctgaggtcat cacttgccgt cctgcagatt tactgacacc tgaaatggca aaactgacca
     3661 gtgaggcaga tcgttttgca aaaactgaag aagatgtgct gacttatgcc atgtttccag
     3721 atattggtaa aacttattta caagagcgca acgctggctc attgcaacct gaggcattgt
     3781 tagacaaaga aactgtcaat gcttcggcgt cacgttttgc cccgagcgag tttaaagtga
     3841 cactgcatgg cgaaaccttc catatcaacc tcactggcag cggccatcca ggggaagaaa
     3901 aacgtccatt ctatgtttct attgatggca ttgcagaaga agtcattgtt gaaacattga
     3961 gtgaaattga agtcacaagt ggtaaaacca gtggcaacaa taaaaagaaa gcggccacga
     4021 gcaaagccag caatggccgc cctcgcccat cgcacgcagg gcatgtaacc acatcaatgc
     4081 caggcacaat tgtggctgta aaagccaaag tgggtgacaa agtgactgca ggcgatggcg
     4141 tgctagtgat tgaagcaatg aaaatggaaa acgaaattca ggcgtcaacg acaggaattg
     4201 tagtcgctgt gcacgtggta aaaggcgaca cagtgacgcc cgatgaggca cttcttgaaa
     4261 ttcaacctga gtaatttact tcagatgtaa tttttaagcc ggcagcatcc acctgtcggc
     4321 tttatttttt gggtttagtg acaatgaaac aaagcgtaaa atcatccgcc aagctaaagt
     4381 cgaagcaaac gctaatcttg gcatcctctt cagagtttcg tcgtgcgctt ttacaaaagc
     4441 tgcaaattcc atttagcagc atttcacccc atgtagatga aacatctctc gaaggtgaaa
     4501 agccgcatga aaccgcgtta agacttgcaa aggaaaaagc caaaaaaatt ggtcaggaat
     4561 acccgcatgc gttgatcatt ggctgcgatc aagtcgccac attagatggg gagcaaatag
     4621 gaaaacccgg caatcacagc aacgcgacaa agcagttgca acgcatgcgc gggcgagagg
     4681 tgacatttca tagcgcgctc tgcttataca atgctgctac gggcaatatg caggctgagg
     4741 ttgtgcctta tctcgttcgt tttaggcagt taagcgatga gcaaattgaa aactatctga
     4801 ataaggagca accttatcac tgtgcgggca gcgcaaaatc tgaaggatta ggcatcgcgc
     4861 ttattgagcg catgcttggt gaagacccca atgcgttaat aggcttacct ttaattaagt
     4921 taatcaatat gctgcaagtt gaagatgtac aggttatcta aaatattttt gaaaatattt
     4981 aaccccatca caatacttga aacgtttaag ctggtgtagc gttcatgtat tgcatgacaa
     5041 gttcaatgtt tgaagcgcta ggtaaccgtt aactttttag gaagaacatc atgccactga
     5101 aatataaact cattgtaatt gcgacacttt caacactcat gacaggcgtt gtagccatgg
     5161 aaaaaaacca aaagaactca gacaataccc caagcagctt ggacgaaacg cgcattactc
     5221 aagatgacca aaaaacggtt gccgtgacta tttacaacca ggatttggca cttgtaaaag
     5281 acacgcgcaa agtgaaccta aaaaaaggtt taaatgcgct ggcgctacgc gatgtgagcg
     5341 cgcagattcg ccctgaaacc gcattgctac gcagcattaa tgcgccaggt agcctcacat
     5401 tactagagca gaatttcgat tttgatttat taacaccaga aaaattactc gaaaaatatg
     5461 taggaaaaac cgttagcatc gtcaaaacgc atccaactac aggcgctgaa accagtgaaa
     5521 aagccactgt actatcagcg aataacggcg tagtgcttaa aatcggcaac cgcattgaaa
     5581 caggtgtttc tggcagaatt gtgtatgatg atgtgcctgc aaatttacgt gaccgcccga
     5641 cattagtgac tcaagtcgac aatagcggcg ccacagacca aacgatggag ctcagctatt
     5701 taacaggtgg actaggctgg aaagcggatt acgtggccga actcagccct aaagaagata
     5761 gtatagactt atcaggctgg gtaacactga ccaataccag cggcgcaaca tataaaaatg
     5821 ccaaattgca gttggtggcg ggtgatgtga accgcgtaca accagaatac ccgcgcgctg
     5881 ccatgatgcg caaaaatgct gatatggtga tggaagcggc agcctccgca cccatggccg
     5941 aagaatcttt actggaatat cacctttaca cgctagaccg ccccactacg attgccgaaa
     6001 gccaaaccaa gcaagtggcg ctactttctg ccagcaatat ccctgctcgc aaagagttgg
     6061 tgcttaaagg cgcagaatat tactacaccg cgcaatatgg cgaaattggc accaaattaa
     6121 aagtaagcac ttttgtggaa tttgaaaaca aagaggctag caaattaggc atgccgttac
     6181 ctaaaggcgt attgcgcgtg tataaaaaag ataaagcagg caatgcgcaa tttgtgggtg
     6241 aagacaacat tgaccacacg cctaagaatg aaacggtgcg cattaaattg ggagaatctt
     6301 ttgacgtaac tgccgataaa aaacaaaccg aatttaaagt gttgcccaac ccgcaaaaag
     6361 gccgcagcgc gtatgaaagc gcatttgagt ttaccttaaa aaatgctaaa aaagagaaaa
     6421 tgaccgtgct tgtgcaagag ccgattggtg gcgattggaa aattatcaac gaaaatcacc
     6481 cacatcaaaa agccaatagt cacttagcga tatggaaaat tgacgtacca gccgaaggca
     6541 aaacaacgct gacttaccgc gtgcaaacca agtattaaaa tgcactaaaa tagatgcttg
     6601 ttagcgccga gaaaaattca ctgccctgca agccttatag aataaggctt gcagggcatg
     6661 tgttttgata tggtgtaaaa tatagacaga atatgtcttc aagaaagccg ctcgcccttc
     6721 acactttact tgccacttta gtggcttagt aaattgggga agccccttgc ggtctgagct
     6781 tatcgaaggg taatttatgt ggttcgacag gctcaccacg aacggatata tttaaactga
     6841 gaagcctccg aagtatcgtt aaattaaaca acaaatcatg agcaacgcac ccgtgaaact
     6901 aggcacctta tattttatcc ccgtcacctt gggtgacgac aattttcaca aagtgttgcc
     6961 gccagaagtg gttgccattg cacaaaaatt agacacattt attgtagaaa atgaaaaaac
     7021 tgcacggcat tttttaagca cgattaaaac agctaaacca gtgcgtgagc tcacattact
     7081 cacgcttaac gaacacaccg aagataagca aattcctgcg ctactcgcgc cattattagc
     7141 tggtatagat gtcgggttga tgagtgaagc aggctgtccc ggcattgccg accctggcgc
     7201 caaacttgcc gcgctcgcgc accctaaagg cattaaagta gcgccgctgg ttgggccaag
     7261 ctcaatatta ctcagcttaa tggcaagcgg cctcaatggg caacgtttta cattcttagg
     7321 ctacattccg caagacaaag ccgcccgcat taaccgccta aaagagattg aaaaacaatc
     7381 cgcaaaaaac aatgaaacac aaatttttat agaaaccccc taccgcaacc agcatttgtt
     7441 agaagatatt ttggcacact gccaagccaa tacaaggttg tgcgtggctt gtaatattag
     7501 ccttgagagt gaagtgattg tgactaaaac gattgcgcag tggaaaaaat caccgctgcc
     7561 tgatttgcat aaaaaaccga cggtgttttt gttaatggcc tagccagctt taataaaact
     7621 tacccataag taagcaacca tcaatagcgc aggcactaca aaataagcga gttcaacatt
     7681 tctacgaagt gttttatatt cttttgagcg ccaatattga gtgacattca caaccccaaa
     7741 caaaatgatg ggcaaagcag aaaccgctgc taacatggtt atcaactggt ttatagccca
     7801 agtgttttcg acccataaat aaccaacaag cagcgctggt atgacgaata tgattgcttc
     7861 aaaatatcgc catgctgaaa cagtaccagt ttgattaaat aaatctttcc aatttaataa
     7921 ataagctaag gtgacaagtg gaaataaacc tatcattaaa aatatagtca acgcagcagc
     7981 tacgcccata taggccatgt ttgcactcgt tgcttgtgag gctgatttag gaaaaaactc
     8041 aagccccgca atataaaatc cagctaaaaa aatcaatagc catatcaaat aagttgcttt
     8101 tctcacacta atcccctgac aatttttttc actggcgcat aactcaaccg atgctcaacg
     8161 caaatcccat gctccgcaag tgctgctaaa tgcgccgccg ttggatagcc tttatgttgt
     8221 gcaaagccgt attgcgggta gattttatcc agttcataca attcagcatc tcgcgcagtt
     8281 ttggctaaaa tactagccgc agaaatcgct tgaaccttgc tgtcgccctt cacaatcgcc
     8341 tcgcacggca ggctgatgtt cgggcattta ttgccatcga cctgcacgaa tatttcaccc
     8401 atttctgttg tcagtttaag ctgtgcttgt aaatcttcta tcgcgcgttt catggctagt
     8461 aagctggctt gcaaaatgtt gatttcatca atttcttgcg cggtacagct tgcaattgcc
     8521 caagctagtg ctttttgttt aatctcaatc gccaatgcat cgcgcctttt ctcgctgagt
     8581 tttttagagt ccgctaaacc aataatgggt tgattcgcat ctaaaatgac ggctgccgca
     8641 taaacgcttc cggccaatgg ccctcgccca gcttcgtcaa cgccacagat tatttttgag
     8701 atttgcatga gatttttata ttcaaaatat gatgattaag tgctctcaac aacacgttaa
     8761 aaaagtgagt tttcgaggtg tttttctacg agagaaaaaa tctcctctgc gagcctttat
     8821 ttataaggct ttcagagcat cgaaaaaggt tgtgcttgaa aattgccttt taatacgatt
     8881 ttttaatgta catttaatca aagattgctg gaaaattgta cgcgatttaa gccaagtatt
     8941 tataagaact ttaagatggc ttctgcagct ttttcagcgg tattttggcg taattggtgg
     9001 tgcatgcgcg taaattcttc tttaatatca gtttgcaacg ctttgtcact caataatttg
     9061 tgtgcagtct cagcgatttt ttcaggcgtc gcgtcttgtt gcaacagttc tggcaccaca
     9121 aatcgctcag ccaaaatgtt cggtaagccc acataaggct gcaagcgcat tcttttaagt
     9181 atttgccagc tcaaattcga catgcggtaa gtaataatca tcggcttttt caaaagcgca
     9241 gcttccaatg tcgcagtacc tgaggcgaca atgattaaat ctgcggcttc catggcatca
     9301 tgggcgtgac caaacattac cgtaatgggt ggacttgggc tgctttctgg caaactgact
     9361 aaaaattgat gcaatttttt tttaaaaatc gcgcgtgttt cacgggtaat caatggcaca
     9421 agaaaaaccg tgtcgtgttt tccttcttca atgagtgact ggttaatgcg atgcgctgct
     9481 tgaataaaca attcagcatg ttgttccact tcaccttgtc ggctacctgg cagcatcgca
     9541 ataacggttt gcgtcgttct tattttaagg ttttctcttg ccccgatgat atctggcaca
     9601 agcggcagta tatcggccag cggatgaccc acataggtca ctggaatgcc ggctttttga
     9661 taaatggcag gctcaaatgg aaacagtgcc agtatgtgcg aaacggcttt ttttatgccg
     9721 ccaatgcgcc ccttacgcca cgcccaaatg gatggactta cataatgaat ggctggaatg
     9781 cctttgtttt ttaatttttt ttcaagccag aagttaaaat ctggcgcatc cacgccaata
     9841 aacaaatcag gtttagttgc taataattgt tgaaataatg cgcgacgaag ctttaataag
     9901 ccaggtaaat gcttaatcac ttctacataa ccgcgtacgg aaagctgctc aattgaaaat
     9961 aaactttttg caccctcgcc tatcatttta gggccggcaa tgccaataaa ctcaatgtga
    10021 ggatggtgtt ttttaagtgc cgccattaaa tggctgccaa gcaaatcccc agaggcttcc
    10081 cctgccacaa tcgcaatttt catgaccatg actttagcgg acgatgccgc gtgtagatgt
    10141 atttaagaaa tcgagcaaca ggccaatttc ttggcagctt ttctgttttt ctgcaagctc
    10201 aagcttcgcc tcttctaagg ttaagccatt gcgatacagc gttttatacg cgcgtttgac
    10261 ttctaagata gcctcagaag aaaagccacg acgcttgagc ccctcggcat tgataccatg
    10321 tggtttagca tcgtaacccg cagccgttac ataaggcgga atgtctttaa acaccaccga
    10381 gcccacagcc gtaatgacat gtgaaccaat tttgcagaac tgatgaatca gagtaaagcc
    10441 gcctaaaatg gcgtaatcgt gtacatcgac atgaccagct aacgatgcat tgttagcaaa
    10501 aatcgtatga ttaccaatca cacaatcatg tgccaagtgc acgtaggcca ttatccaatt
    10561 atcgttgcca atttttgtcg tgcctttgtc ttgcacggtg ccacgattaa acgtgcaaaa
    10621 ctcacgaatg gtattgttat caccaatttc gagtaaggtt ggttcattct tgtatttttt
    10681 atcctgcggc gcttcaccca gtgacgaata ttgaaaaatc tgattatgct tgccaataat
    10741 ggtgtggtcg ctgatagtga catgcccggc aatgcgcgtg ccagcatcaa ttttaacgtt
    10801 tggcccaata atcgaaaacg gacccacctc aacgctggag tctaactctg catttttatc
    10861 aataatagca gtggcgtgaa tttttgcaga catttaacaa tttactttaa tttataatta
    10921 tatattattg attggtatta cttctctatc gcctttaaaa tgcacatcat gctcgcttca
    10981 gccaccactt cattatctac agacgcactt ccagtgtatt tccatattcc tttaagaatg
    11041 cggtcaattt taacgttcaa cacaatttga tcgcctggtg acactggttt tttaaatctt
    11101 gcactgtcaa tacccgcaaa ataatagacg gactcatcat taggttttgc atccatggtt
    11161 ttaaatgaca acaccgcagc cgcttgtgcc atggcttcaa caattaaaac gcccggcatg
    11221 actggatgat acggaaaa
//
LOCUS       DAOQSI010000056        11014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_5162_length_11014_cov_5.179943, whole genome shotgun sequence.
ACCESSION   DAOQSI010000056 DAOQSI010000000
VERSION     DAOQSI010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 11014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11014
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5162_length_11014_cov_5.179943"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..571)
                     /gene="queF"
                     /locus_tag="PKL42_05135"
     CDS             complement(113..571)
                     /gene="queF"
                     /locus_tag="PKL42_05135"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HNU66492.1"
                     /translation="MSITTSSTSLGAKPTAQPTKSLETFENPKQSRDFHIHMEIPEFT
                     CLCPKTGQPDFAVIYLDYIPDALCVELKSLKLYMWSFRDEGCFHEAVTNQILDDLVAA
                     TQPKFMRVTAKFYVRGGVFTNVVAEHRKPGWQPLPRVDLTQFDSQSNIRG"
     gene            638..4171
                     /gene="smc"
                     /locus_tag="PKL42_05140"
     CDS             638..4171
                     /gene="smc"
                     /locus_tag="PKL42_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNU66493.1"
                     /translation="MRLTHLKLAGFKSFVDPTTLHIHGQRVGVVGPNGCGKSNVMESV
                     RWVLGESSAKEMRGDSMDAVIFNGSGNRKPISRASVELIFDNSLGGASGEWSQYAEIS
                     VKRVIERDKGSTYYINNTVVRRRDVADLFLGTGLGGRAYAIIGQNTINRIVEAKPEEL
                     RIFLEEAAGISKYKERRRETELRLRDTRENLTRVEDILRELDKQIARLQSQAVVAEQY
                     HQLQAALNVSKGQVWLLKKRDASTQWEKAQRNVERLVNELEAQMAALRSNESVLEIAR
                     QENITANESVNLAQASYYEINAEVSNLENQVKNTADARERIQQQLMQLVASLEKNAVQ
                     SQHYKNMSLSAQQALEVANTEGTAAEQAVAQVKGALPALLAQFELAATTFTTRQTALL
                     NAEQRLRLEQTNLDHISRAIRETQDQLSRLVQNLNSIQITSEEAIDEKQSALRLAETE
                     VAKLEAQVTSVLENEQSLGARIKVQRDLYAEKQRDLSHLEAEIASLTKIQHSMQNGQS
                     ESALKGWLNSVGLTDEVRFWQSVRVKKGWEAAFEAALGAKLNAIPHASLNIQTRPPGA
                     LTIALDSDETSDVIQRENSLYSLVENIHPKQRGTLQDWLAGAYILENGVNIEDARRGL
                     SSGEYLVSKQGDIYTQHSVTYFGSQSLLHGVLERQAHLEALEKQKPLAVQTVADALDQ
                     VTQTENALRQLRDVQRESSVLLKSALQNQHQLNLTLQQLKQTQSNAIQRQKSLQSDYK
                     VMEEKLQKLNDERGAKETIIHDITQSLQKIQQDKTIAEANKVQAEMAFNQARTQLQSL
                     EHAFQEKSFNIKIKQNEINELNSKMQHADEECASLKLRRDEAEATLNVTQMDTLKLNL
                     EVALNRKQQAEQLLASARNQLSVCEQALLQQERLRMQNEQLLHPLRDKLETSRLEEQE
                     TRLHFELCQAELSASHLSETQLAEHLEALASKTADGKLKVIDLEKRRDQIERQITELG
                     AVNLAAIQELESEQTRKQYLDSQCKDLMDASETLEDAIRKIDKETRGRLQATFDEANR
                     HFSELFMTLFGGGQARLELLGEEILDTGMQVFAQPPGKKNSTIHLLSGGEKALTALAL
                     VFALFRLNPAPFCLMDEVDAPLDDSNTERFCTMVKKMSEKTQFLYVSHNKITMEMAQQ
                     LIGVTMQESGVSRIVDVDMEQAVKIAEVASA"
     gene            4228..5691
                     /locus_tag="PKL42_05145"
     CDS             4228..5691
                     /locus_tag="PKL42_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HNU66494.1"
                     /translation="MSNLYILLIVIGVLIIVAVLAYNWWQERKFNQQVDSGFSALQKD
                     ALLDDPEIDSNQVKSGFEDTIIDDFAIKHQKSVNESQPASQPTPQYQTQASPSANNPL
                     ENSIADQILPDDVSINDAFSELTNALKGTQTRKVEPSFNAANLASTASNVNAPEKPVQ
                     RSDIKAIFDDAFGKTNKAPSANIGNTAPEQKSRPTANIVNEIEKPPLGLPAMLQAQMD
                     LTAVLHLAKEIPASLLIKTVAGLFNDYDKPIFVHTIATRVNANPSWLLLSETPEKQLI
                     AKIACSLQLADRAGAVSRNVLNRFQQAVETLGKGLNAHVEWQSNGDALTTATALDTFC
                     IEVDKTIGFHLVHGESGAFTGTKLKGLAEAQGFKLAADGSFKFYNDEGRVAFIMFNRD
                     NFPFSQDMLRNSVIKGITFQLDIPHVLHCAEAFNQMVEIARLMESSLHATLVDDNNRV
                     LGDIQVEKIRQQLKVIHATMLVRGIIPGSDNAIRLFS"
     gene            5728..7734
                     /gene="ligA"
                     /locus_tag="PKL42_05150"
     CDS             5728..7734
                     /gene="ligA"
                     /locus_tag="PKL42_05150"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNU66495.1"
                     /translation="MLEEPTQQIHALRKKITQYDYEYYVQDAPSVPDSEYDKLYRELQ
                     YLEQQFPQLIKPDSPTQRVSGTAVNAFNSITHRQAMLSLNNAFEALEVEAFDKRVRDT
                     LGQALVEYAVEPKFDGLAITLTYEDGVFTQGATRGDGYTGEDVTHNLRTIHSIPMRLD
                     VTHPPQLLEVRGEVLMLKRDFEKLNLAQSNKGEKLFANPRNAAAGSLRQLDAKITATR
                     PLHFFAYGLGAAEGVPSITSHSMAMDYLARLRFPVCAERGVVKGLAGLLEYYKSVGLA
                     RDTLPFDIDGVVYKVNQFSQQNELGFVSRAPRWAIAHKFPAQEALTTVEDITVQVGRT
                     GAITPVARLAPVFVGGVTVTNATLHNEDEIRRKDVQIGDTVSVRRAGDVIPEVVSVVL
                     EKRPANARRFEMPQVCPECGSHILKQADEAVARCTGGLFCPAQRKQAIIHFASRRAMD
                     IEGLGEKLVDQLVEAHLVHSLADIYTLDLATLSSLERMATKSAQNILDALQHSKTTTL
                     ARFIYALGMRNVGETTSKDLAKHFGNLSALMQANTEALLEVNDVGPVVAESITNFFSE
                     THNQEVIAALLNAGITWPETDGKQQATGQLVGKTFVLTGTLPTLSREAAKELIEQAGG
                     KVSGSVSKKTDFVVAGSDAGSKLDKAQELGVAILDEAGLQQLLS"
     gene            7845..>8073
                     /locus_tag="PKL42_05155"
     CDS             7845..>8073
                     /locus_tag="PKL42_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66496.1"
                     /translation="MNLYSTLSYTFILLILNSSIAFSAEPESCQQALQKGDVAQALST
                     AKKMLAVNAKDVDTLLCQGRAFTASQDFNSAL"
     assembly_gap    8074..8173
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8174..8902
                     /locus_tag="PKL42_05160"
     CDS             <8174..8902
                     /locus_tag="PKL42_05160"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNU66497.1"
                     /translation="LLCQGRAFTASQDFNSALDAFKLAQLNASDSTQKTIAALLIGNA
                     QLSLKQPDLAIASFQQALEHAKTTNSATYVRASHLALGNAYLNQQQFEPALAQYQAVN
                     KLDANDNERGESFEKIALTHHKMGQHDAALEFQIKAYMMHEKSGTLDQYAHSSIELGR
                     YYGQAKQYIRAENTLNKIIKFAKEQGGAYYEAKGSCVLGIVKAAEGEKEAAKALVEYA
                     KSIAKNTQDKSLEEEINQETQGLL"
     gene            9120..10115
                     /gene="msrP"
                     /locus_tag="PKL42_05165"
     CDS             9120..10115
                     /gene="msrP"
                     /locus_tag="PKL42_05165"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HNU66498.1"
                     /translation="MKPYSPPYIPASEITPKDTFENRRAFLQKAGFGVAAGTLALLNH
                     PVRAAETAVKSSIIQPVRAGKKIAQYKKTAFGSEEKLTSYEDVTTYNNFYEFGTDKSD
                     PAKNAKLFKTQPWAVSIEGEVKKPKIISLEDIIKLAPLEERIYRMRCVEGWSMVIPWL
                     GIPLAKLIQWAEPTGNAKFIEFITLNDKQQMPGQNLPVLDWPYVEGLRMDEAMHSLTM
                     LAVGLYGEQLPNQNGAPLRLVVPWKYGFKGAKSIVKIRFTEKMPRTSWMKAGPREYGF
                     YANVNPNVDHPRWTQSSEKRIGAGLFAGRIKTQMFNGYAEQVGQMYAGLDLQKNF"
     gene            10335..10958
                     /locus_tag="PKL42_05170"
     CDS             10335..10958
                     /locus_tag="PKL42_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HNU66499.1"
                     /translation="MLHFFKKTLNKQQIARVKVLVFLLCTIPMLRLIWLGFQEDLTAN
                     PIEFLERSTGFWALMILLATLTLTPIRIITGRAWPLQMRRMLGLWMFFYACCHVIIYV
                     WLDFAFLWADIVKDIAKHPRILVGFAAFVLTIPLAATSNQAMMKRLGQHWKPLHQSVY
                     VIAILSIVHFWWLVKIDIREPMFYALVLAILLGIRIYYQFKSKHKIK"
BASE COUNT         3316 a         2228 c         2562 g         2808 t
ORIGIN      
        1 cccagttacc tacattcacc acttacttaa ttcagtaatc agcgaggcgc aatattaaag
       61 atgagaggct aggttgtcaa tgacttttgt gaggtttatg taaaatcatc cgttagccac
      121 gtatatttga ttggctgtca aattgggtta aatcaacgcg cggtaaaggc tgccaaccag
      181 gtttacgatg ttcagcaacc acattggtga atacgccgcc acgcacataa aattttgccg
      241 tcacgcgcat aaattttggc tgtgtcgctg caaccagatc atctaatatc tggttggtga
      301 ctgcttcatg aaagcagcct tcatctctga atgaccacat atagagtttt aaacttttaa
      361 gttcaacaca taatgcgtct ggtatgtaat ccagataaat gacggcaaaa tcaggttgtc
      421 ctgttttagg gcacaagcat gtaaattccg gtatttccat gtgtatgtga aaatcacggg
      481 attgttttgg attttcaaac gtttccaatg acttagtagg ttgtgcagtc ggtttggcac
      541 ctaaagaagt gcttgaagta gtgatagaca taaggcatct tagtaaaaaa aacgttatta
      601 taacgctcta acgaaaacct aaaattaaaa aatcaatttg cgcttaacac acttaaaact
      661 cgcaggcttt aaatcatttg ttgatccaac cacattgcac attcacgggc aacgtgttgg
      721 cgtggttggc cccaacggtt gcggtaaatc taacgtcatg gaatctgtgc gctgggtgtt
      781 gggtgagtct tctgctaaag aaatgcgcgg agacagtatg gatgccgtga tttttaacgg
      841 ctctggtaat cgcaaaccta tttcacgtgc aagcgttgag ttgatatttg acaatagttt
      901 aggtggtgcg tcaggtgaat ggtcgcaata tgccgaaata tcggttaaac gcgtgattga
      961 gcgtgacaaa ggctcgactt actacattaa caataccgtt gtgcgacgcc gtgatgttgc
     1021 tgatttattt ttaggaactg gcttaggtgg ccgcgcctat gcaattattg gtcaaaatac
     1081 cattaaccgt atcgttgaag ctaaacccga agagttgcgt attttcttgg aagaggcagc
     1141 aggtattagt aaatataaag aacgcagacg ggagactgaa ttgcgtttgc gtgacacgcg
     1201 cgaaaattta acccgtgtag aagatattct tcgagaactt gataagcaaa tagctcgttt
     1261 acagtcacaa gcagtggttg ccgaacagta tcatcaattg caagccgcat taaatgtgag
     1321 taaaggtcaa gtttggctgc taaaaaaacg tgatgcaagt acgcaatggg aaaaagcgca
     1381 acgcaacgtg gaaagacttg tgaatgaact tgaagcacaa atggcggctt tgcgcagtaa
     1441 tgaatcagta ttagagattg cacggcaaga aaacattaca gccaatgaat cggtaaatct
     1501 ggcacaggcc agttattacg agataaacgc tgaagtttca aacctagaaa atcaggttaa
     1561 aaatactgcc gatgcgcgtg agcgcattca acagcagttg atgcagttag tggcctcatt
     1621 agaaaaaaat gctgtgcaat cgcaacatta taaaaatatg tctttgagtg ctcaacaagc
     1681 gcttgaggtt gccaatactg aaggcactgc tgctgaacag gcggttgccc aagttaaagg
     1741 tgctttgcca gcattgttgg cgcaatttga gctagctgca acaacattta ctacacgtca
     1801 aacggcgtta cttaatgccg agcagcgctt acggcttgag caaacaaatc ttgaccatat
     1861 ctcacgtgca attagggaga cgcaggatca gctgtcacgg ttagtacaaa atttaaatag
     1921 cattcaaatc acttctgaag aggccattga tgaaaagcaa tctgcgctca gattagctga
     1981 aacagaagtc gctaaattag aagcgcaagt cacctcagtg cttgaaaatg aacagtcact
     2041 tggcgcacgc attaaagttc agcgtgatct ttacgctgag aagcagcgtg atttaagtca
     2101 tcttgaggct gaaattgcct cacttactaa aattcagcac agtatgcaaa atggtcaaag
     2161 tgaatctgca ttgaaaggct ggttaaatag tgttggtttg accgatgaag tgcgtttttg
     2221 gcaatcagtg cgtgttaaaa aaggttggga agccgcattt gaagcagcct taggcgcaaa
     2281 actcaacgct atacctcatg cgtcactgaa tattcaaaca cggccgccgg gcgcgctcac
     2341 gattgctctg gattctgatg aaacaagtga cgtgatacaa cgagaaaact cactctattc
     2401 tttggtggaa aatattcacc caaagcagcg cggcacgcta caagactggc tagccggtgc
     2461 gtatattctg gaaaacggag tgaatatcga ggacgctcgt cgcgggttat ccagtggtga
     2521 atatttagtg tccaaacaag gtgatatcta cacgcaacac agcgtgactt attttggctc
     2581 acagtcactg ttgcacggtg tacttgagcg gcaggcgcac ttggaggcat tagaaaaaca
     2641 aaagccactt gctgtgcaaa ctgtggcaga tgcgctagac caagtgacac aaacagaaaa
     2701 cgctttgcgt caattacgtg atgtgcaacg cgaaagtagc gtgttactta aaagtgcttt
     2761 gcaaaatcag catcagctca atttaacact gcagcagtta aaacaaaccc agtctaatgc
     2821 aattcagcgg cagaaaagct tgcaaagtga ttacaaggtc atggaagaga aattgcagaa
     2881 gttaaatgat gagcgtggtg caaaagagac gattatccat gacataacgc aaagtttgca
     2941 aaaaatacag caggataaaa ccattgctga agcaaataag gtacaggcag aaatggcgtt
     3001 taatcaagcg cgcactcaat tgcagtcgct ggaacacgct tttcaagaaa aaagttttaa
     3061 catcaaaatt aagcaaaacg aaatcaatga attaaattct aaaatgcagc atgctgatga
     3121 agaatgtgcc agcttgaagt tacgtagaga cgaagctgaa gccacgctca atgtgaccca
     3181 gatggacacg ctaaagctga atttagaggt ggcattgaat cgtaagcagc aagccgaaca
     3241 gttgctggct agcgcacgta accaattaag cgtatgtgag caagcgttgc tgcaacaaga
     3301 acggctacgt atgcaaaatg agcaattatt gcacccactg cgtgataaat tagaaaccag
     3361 ccgcttagaa gaacaagaaa cacgcttaca ttttgaattg tgccaagctg aattgtctgc
     3421 gtcgcatttg agtgaaactc agcttgcaga acacttagag gctttggcta gtaaaactgc
     3481 tgacggtaaa ttaaaagtaa tcgatttgga aaagcgacgc gatcaaatag agcggcaaat
     3541 taccgaatta ggcgcagtca atttggccgc tattcaagag ttggaatccg aacaaacgcg
     3601 aaaacaatat ttagatagcc aatgcaaaga cctaatggac gctagcgaga cgcttgaaga
     3661 tgcgattcgc aaaatagaca aagaaacaag agggcgtttg caggccacgt ttgatgaggc
     3721 caaccgacat tttagtgagc ttttcatgac cctgttcggt ggtggccaag cgcgtttgga
     3781 gttgctcggc gaagaaattc tagataccgg catgcaagtt tttgcgcaac cgccgggtaa
     3841 gaaaaatagc accatacatt tactttctgg tggcgaaaaa gcattaacag cgcttgcttt
     3901 agtgtttgcc ttgtttaggt taaatccagc gccgttttgt ttaatggatg aggtagatgc
     3961 accattagac gatagtaata cagaacgttt ttgtacgatg gtgaaaaaaa tgtctgagaa
     4021 aacacagttt ttatatgtaa gtcataataa aataaccatg gaaatggcgc agcaattgat
     4081 tggtgttact atgcaagaat caggcgtttc acggattgta gatgtggata tggagcaggc
     4141 tgtaaaaatc gccgaagtgg ccagtgctta aaattgaata tttttactgt taagtaataa
     4201 aaaacaacta tttgaaagtc agcttaaatg agtaatttgt atattttgtt aatcgtcatt
     4261 ggcgtactga ttattgttgc agtactggcg tacaactggt ggcaagagcg aaaatttaat
     4321 cagcaagtgg atagtggttt cagtgcattg caaaaagatg cgctattaga tgacccggaa
     4381 attgattcaa atcaggtgaa aagtggtttt gaagatacca ttattgatga ttttgcaatt
     4441 aaacatcaaa aaagtgttaa tgaatctcaa ccagctagcc aacccacacc gcaataccaa
     4501 acgcaagctt cacccagtgc aaacaatccg cttgaaaatt caatcgccga tcaaattctg
     4561 cctgatgatg tgtcgattaa cgatgcattt tccgaactaa ccaatgcgtt aaaaggcacg
     4621 caaacgcgta aagttgagcc tagttttaat gccgcaaatt tggcctcaac agcgagcaat
     4681 gtgaatgcgc cagaaaaacc ggttcagcgc agtgatatta aagcgatttt tgatgacgca
     4741 tttggaaaaa ccaacaaagc gccgtctgcc aacattggta acactgcacc tgagcaaaaa
     4801 agccgaccta ccgccaatat tgtcaacgaa attgaaaagc cgcccttggg tttacccgca
     4861 atgctacaag cacaaatgga cttaactgca gtattgcatt tagccaaaga aatcccagca
     4921 agcttgctca ttaaaacggt ggcagggctg tttaacgatt acgacaagcc catttttgta
     4981 cacaccatcg ccacgcgtgt gaatgccaac ccaagctggc ttttgctgag tgaaacgccc
     5041 gaaaagcagc tgattgcaaa aattgcctgt agcttgcaac tggctgaccg tgctggcgca
     5101 gtttctcgca acgtgctgaa ccgctttcaa caggctgttg aaacactagg caaaggttta
     5161 aatgcgcatg ttgagtggca aagtaacggt gacgcgctca caacagccac cgcattagat
     5221 acattttgta ttgaagtgga taaaaccatt ggttttcatc tggtgcacgg cgaaagcggg
     5281 gcgtttaccg gcactaaact gaaaggtttg gccgaggcgc aaggctttaa acttgcggcg
     5341 gatggcagct ttaaatttta caatgatgag ggtcgtgtgg ctttcattat gtttaatcgg
     5401 gacaactttc cattcagtca agacatgctg agaaactctg tgattaaagg cattactttt
     5461 cagctggata ttccgcatgt actgcattgc gctgaggcgt ttaaccaaat ggtggaaatt
     5521 gctagattaa tggaaagcag cctgcatgcc acgctggtgg atgacaataa tcgggtgttg
     5581 ggcgatattc aggttgaaaa aatccgtcaa caacttaaag taatccatgc caccatgctg
     5641 gtgcgcggca ttattcctgg ttctgataac gcaatcagat tattctcata agacgtttca
     5701 gtcgcttcgc aaattttaga gtttagtatg cttgaggagc caacgcagca gattcacgca
     5761 ctacgcaaaa aaataacgca gtatgattac gaatactacg tgcaggatgc gccctccgtc
     5821 ccggacagtg aatacgataa gctttatcgt gagttgcagt atttagagca gcaatttccg
     5881 caactcatca agcctgattc tcctacacag cgcgtaagcg gcaccgcagt aaatgcattt
     5941 aatagcatta cccatcggca agccatgctg tcactcaaca atgcttttga ggcactcgaa
     6001 gttgaagctt tcgataaacg cgttcgagac accctagggc aagcacttgt tgaatatgct
     6061 gtagaaccta aatttgatgg tttagccatt accctaactt acgaggatgg tgtgtttaca
     6121 caaggtgcca cgcggggcga cggctacaca ggtgaagatg tcacgcataa tttacgcacc
     6181 attcactcaa tccccatgcg tcttgatgtg acccatcctc cacaattatt agaagtgcgt
     6241 ggcgaagtgc tcatgctgaa gcgcgatttt gaaaaattaa atttggcgca atcaaataaa
     6301 ggggaaaaac tttttgcaaa cccgcgcaat gcggcggctg gcagtttgcg tcagctagat
     6361 gcaaaaatta ctgcgacccg acccttgcat ttttttgcct atggtttagg tgcggcagaa
     6421 ggcgtgccga gtatcaccag ccacagcatg gcgatggatt atttagcgcg tttacgtttt
     6481 cctgtctgcg ctgaacgtgg tgtagtcaaa ggtttggcag gactgctgga atactacaaa
     6541 agcgtggggt tagcgcgcga tacattaccc tttgatattg atggtgtggt gtataaggtt
     6601 aaccaattca gccagcagaa tgaattaggc tttgtatcac gggcgccgcg ctgggcaatt
     6661 gcccataagt ttcctgcgca agaagcactt accacggttg aagacattac cgtgcaagtg
     6721 gggcgcaccg gtgcaattac gccggtggca agattagcgc cagtgtttgt gggcggcgtt
     6781 acggtaacga acgctacttt gcataatgaa gatgaaattc ggcgcaaaga tgtgcagatt
     6841 ggtgacaccg tcagcgtgcg cagggcaggg gatgtcattc cagaagtcgt cagcgtggtg
     6901 cttgaaaaac gcccagccaa cgcacgccgc tttgaaatgc cgcaagtttg cccagagtgt
     6961 ggctcgcata ttctaaagca ggctgatgaa gccgttgcgc gttgtacggg tggcttgttt
     7021 tgcccggctc aacgcaagca ggccatcatt cactttgcct cacgtcgcgc gatggatatt
     7081 gaaggcttag gcgaaaaatt agttgaccag ctagtagaag ctcacttggt gcacagtctg
     7141 gcagacattt atacactaga tttggcaacg ttaagtagcc ttgagcggat ggcaacaaag
     7201 tcagcgcaaa atatactgga tgccttgcaa catagtaaaa caaccacgct ggcacggttc
     7261 atttatgcgc tgggtatgcg taatgtgggt gaaaccactt caaaagacct tgccaagcat
     7321 tttggtaatt tatcggcgtt aatgcaagcc aatacggaag cactgcttga agtgaatgat
     7381 gttggccctg tggttgctga atccattacc aattttttta gcgaaacgca taatcaagaa
     7441 gtcatcgcgg cgctactaaa tgctggcatc acgtggccag aaaccgatgg caaacagcag
     7501 gcgacaggcc aattggttgg taaaacgttt gtgttaaccg gcacgcttcc tacgttatcg
     7561 cgtgaggccg ccaaagagtt gattgaacaa gccggcggaa aggtcagcgg cagtgtctcg
     7621 aagaaaacgg attttgttgt ggcaggaagc gatgccggca gcaaactcga caaagcgcaa
     7681 gaattgggtg tagcgatatt agatgaggct ggcctccagc aattgcttag ttaagttgct
     7741 agcttatgtg attattggcg aagttgaaag ttgaaccgag cgttcaaaga ttagtctagc
     7801 taacgttgtt ttggaagata ttttattggg tgattgggtg aaaattgaat ctatattcta
     7861 cattaagtta tacttttata ttattgattt taaatagttc tatagcattt tcagcagagc
     7921 ctgaaagctg tcagcaagct ctacaaaaag gcgatgtcgc gcaagcatta tctaccgcaa
     7981 aaaaaatgct cgctgtgaat gctaaagatg ttgatacgct gctgtgccaa ggccgcgcat
     8041 tcactgcaag tcaagatttt aacagcgcac tggnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnctgctgt gccaaggccg cgcattcact gcaagtcaag attttaacag
     8221 cgcactggat gcatttaaac tcgcacaact gaacgcaagc gacagcacgc agaaaacaat
     8281 tgcggccttg ttaatcggta atgcgcagct atcactcaag caaccagatc tggcaattgc
     8341 gagtttccag caggcattag aacatgcaaa aacgaccaat agcgccacct acgtgcgtgc
     8401 aagtcattta gccttgggga atgcctattt gaatcagcag caatttgaac cagcattggc
     8461 gcagtatcag gccgtgaata agctggatgc caacgacaat gaacgtggtg aaagttttga
     8521 gaaaattgcc ttaacgcacc acaaaatggg ccagcatgat gccgcccttg agtttcaaat
     8581 taaggcttat atgatgcatg aaaagtccgg cacactagac caatatgcgc attccagcat
     8641 tgaattaggg cgctattatg gccaggcaaa acaatatatt cgcgctgaaa acaccttaaa
     8701 taaaatcatt aaattcgcaa aagagcaggg cggggcttac tatgaagcaa aaggtagctg
     8761 cgttttaggc attgtaaaag cagctgaagg tgaaaaagag gcggcaaaag cgttggttga
     8821 atacgcaaaa tcaattgcaa aaaatacgca ggataagtca cttgaggaag aaattaatca
     8881 agaaacgcaa ggcttgttat aagcgcaaaa ttgctacgag taaaaattac tgccctgcga
     8941 gcctttattt acaaggctta cagagcatcg aaaaaggttg tatggggttt ttgctagtaa
     9001 aaatgtattt tttctacaaa attgcaatga gttccagcat aaaaatttgt aaggcttatg
     9061 caaatatggc gaccaagtat tcagccgcca aacatcacca tgacaaataa ggcaatgcca
     9121 tgaaaccata ttcgccaccc tatattccag cttctgaaat cacacccaaa gacacctttg
     9181 aaaaccgtcg cgcatttttg caaaaagccg gatttggcgt tgcggcaggc actcttgcgc
     9241 tgttgaatca tcccgtgcgt gcggcagaaa ccgccgtaaa atcgtccatt attcagccag
     9301 ttcgcgcagg taaaaaaatt gcgcaatata aaaaaaccgc ttttgggtcg gaggaaaagc
     9361 tgacttcgta tgaagatgtc acgacctata ataatttcta cgagtttggc accgacaaaa
     9421 gtgatccggc caaaaatgcc aagctgttta aaacacaacc ttgggcggta agtattgaag
     9481 gtgaagtaaa aaaaccgaaa atcatcagcc ttgaagatat catcaaattg gcgccccttg
     9541 aagagcgcat ttaccgtatg cgctgcgttg agggctggtc catggtgatt ccatggctgg
     9601 gcattccttt agctaaattg attcaatggg cagaaccaac gggcaatgct aaatttattg
     9661 aattcatcac gttaaacgac aaacagcaaa tgcccgggca aaacttaccg gtgctggatt
     9721 ggccctatgt tgagggtttg cgcatggatg aagctatgca ttcgttaaca atgctggcgg
     9781 ttggcttata tggcgaacaa ttgcctaatc aaaatggcgc gcctctgcgc cttgtagtgc
     9841 cgtggaaata cggttttaag ggcgcaaaat cgattgtaaa aattcggttt acagaaaaaa
     9901 tgccacgcac cagctggatg aaagccgggc caagagaata tggattttat gccaacgtga
     9961 acccaaatgt tgatcatccg cgctggacgc aatcttcaga aaaaagaatc ggtgctgggc
    10021 tttttgcagg gcgtattaaa acgcaaatgt ttaacggcta tgccgagcaa gttgggcaaa
    10081 tgtatgcggg tttggatttg cagaaaaact tctaattttt ctcaacttat caatacgata
    10141 cggtaataga aagagggcgg ataaaaatgc aaaaaagtgc catttttaga agcaaccttt
    10201 ttcgatgcca tgcgagccct ataaacaaag gctcgcaggg cataaaaatt ttctcgtaag
    10261 aataacgcct agtttttgaa tgtgataatt gcgtttaaaa aagcacccac tctattgaaa
    10321 cttactggtt aagcatgttg catttcttca aaaaaactct gaataaacaa caaatcgcgc
    10381 gcgttaaagt gttggtgttt ttgctctgta ccatcccaat gttgcgcttg atttggcttg
    10441 ggtttcaaga agatttaaca gccaacccaa tagaatttct tgaacgttca accggttttt
    10501 gggcgctcat gatattacta gcaaccttaa cacttacccc catccgtata atcactggca
    10561 gggcatggcc gttgcaaatg cggcgtatgc tagggctgtg gatgtttttt tacgcttgct
    10621 gccatgtcat tatttatgta tggcttgatt ttgccttttt atgggctgat atcgtcaaag
    10681 atatcgctaa acatccacgt atattggtag gttttgcggc ctttgtgctc accattcctt
    10741 tagccgcgac ttcaaatcaa gccatgatga aacgcttagg ccaacactgg aaaccgttac
    10801 atcaaagtgt ttatgtcatc gccatattaa gtattgtgca tttttggtgg cttgtaaaaa
    10861 tagatattcg tgaacccatg ttttacgcac ttgttttagc tattttgctg ggtatacgta
    10921 tttattatca atttaaatca aagcataaga taaagtaaat attataacta attgaataat
    10981 ataaacatta aatatattga taaaattgct tgta
//
LOCUS       DAOQSI010000057        10936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_5235_length_10936_cov_6.091352, whole genome shotgun sequence.
ACCESSION   DAOQSI010000057 DAOQSI010000000
VERSION     DAOQSI010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 10936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10936
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5235_length_10936_cov_6.091352"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /gene="coaBC"
                     /locus_tag="PKL42_05175"
     CDS             complement(<1..1136)
                     /gene="coaBC"
                     /locus_tag="PKL42_05175"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNU66500.1"
                     /translation="MTISTNTNIRRKKLVLGITGGIAAYKAAELVRLLVKAEIDVQVV
                     MTTAACQFITPVTMQALSGKPVFMDMWDSSISNGMPHIELSREADAILIAPASADFLA
                     KLVHGRADDLLSTLCLARDCPLLVAPAMNKQMWEAPATQRNIAQLSADGVTILGPESG
                     VQACGETGLGRMLEAETLLAHINSHFTPKLLANKRVIITAGATIEKIDPVRAITNLSS
                     GKMGYAIAQAAADMGAEVTLVSGATALATPHNVTHISATSAENMYQAVMKNIANLKFG
                     PKDIFISVAAVADYSPAQTSTQKIKKSEQTLTIALTKNKDILAEVASLPNAPFCVGFA
                     AESENLLEYAEAKRLAKKLPLIVANLATDAMGSDDNQVTLLDKNG"
     gene            1251..2018
                     /locus_tag="PKL42_05180"
     CDS             1251..2018
                     /locus_tag="PKL42_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66501.1"
                     /translation="MMWKKIRIFILLLVFGAVVQQTMIEQSDLDWDRNFYVAVYPINA
                     DGTTAVDHYISTLTKEDFEPIAEYFSEEVKRYGLTMRRPFQVQLGAKVLRQPPAPPKA
                     ASILDTITWSLKFRYFSWKNSPQVAVKPKIRMYLLYFDPTKNARLSHSTALSKGRVGY
                     VNLFGSKAAHRQNMVILAHEMLHTLGATDKYNLTTTLPIFPEGYAEPNKTPRYPQDFA
                     ELMGGRVPISESQAEIPKSLLHTLVGENTAKEIGWIK"
     gene            2083..2757
                     /gene="radC"
                     /locus_tag="PKL42_05185"
     CDS             2083..2757
                     /gene="radC"
                     /locus_tag="PKL42_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNU66502.1"
                     /translation="MAITDWPAAERPREKLIEQGAGALSDAELLAIFLRVGVVGKSAV
                     DLARDLLTQFGSLNGIFAATEHELSQVHGIGSSKYVQLQAIFEMSRRALSEQLQQRDV
                     LSSPQHVRDYLVLKLASLTREVFMVLFLDTQNRLLASEELFSGTLSQASVYPREVIKR
                     VLHHNAASVIFAHNHPSGIAQQSAADELLTKQLKEALTLVDVGVLDHFIVAGNQTLSF
                     KERGLL"
     gene            2804..4873
                     /locus_tag="PKL42_05190"
     CDS             2804..4873
                     /locus_tag="PKL42_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HNU66503.1"
                     /translation="MNKYFRILIWVAVALLGAFAISGIALNRGESINALWLITAAVCV
                     YAIAYRFYAAWIAAKVLVIDETRATPAERLNNGRDYIPTNKWVVFGHHFAAIAGPGPL
                     VGPTLAAQFGYLPGTLWILIGAVLGGAVQDMVTLFISTRRDGRSLGQMARDEIGAIGG
                     TAALVGTFLIMIILIAVLGLVVVNAMKHSPWATSTVAATIPIAMIVGVYMRNIRPGRV
                     LEATTLGVVLLLFSVFAGGWIDHHETLRTFFDHDGLPLAFAIIVYGFAAAVLPVWLLL
                     APRDYLSTFVKLGTIALLAVAIVILRPDIHMPALTQFTDGTGPIFGGKLFPFVFITIA
                     CGAISGFHALISSGTTPKLLSNERDIRMIGYGAMLLESFVAIMAMIAATVLEPGIFFA
                     INSPAGVVGKEAVDAVAKINSWGYAVTVEQMNQLAKDMGESSLFARTGGAPSLAVGMA
                     SIFGSAFGKGMLALWYHFAIMFEAIFILTTLDAGTRVGRFMLQDMLGNFNKKLGETSY
                     MPSVILTSAIVVAGWGYFLYIGVIDPNGGVNILWPLFGIANQMLAAIALCVATGVLVK
                     SGKLKYAWVTGLPLFWLVIITTTAAYEKIFSSDIRVGFFAAANDLSTKLAAGILPPEK
                     AAVAPQLIFNQHLDAYLTLFFVVVLWVVLLDMLRLCVRYLAGKPVAALSETPREVTQL
                     ENAWVRD"
     gene            4883..5074
                     /locus_tag="PKL42_05195"
     CDS             4883..5074
                     /locus_tag="PKL42_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HNU66504.1"
                     /translation="MLKKLKHLWHIVRRLTGDDAYEVYLKHHAAFHQSALDAPPPLSR
                     KEFFKIWQDSQWKDIKRCC"
     gene            5065..5331
                     /locus_tag="PKL42_05200"
     CDS             5065..5331
                     /locus_tag="PKL42_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03643 family protein"
                     /protein_id="HNU66505.1"
                     /translation="MLLEHSLSEEDVSRIIEMAWEDRTAFEAIAVQFNLNQDGVIKLM
                     RSQLKPNSFKRWRARTRGQVTKHLHLRVAGVSRHQSRQHNKLHR"
     gene            complement(5344..5532)
                     /locus_tag="PKL42_05205"
     CDS             complement(5344..5532)
                     /locus_tag="PKL42_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66506.1"
                     /translation="MKNLIDKLFKKESELNYILPQYQPAQFDLISTRVVNKEYDLKDP
                     AESFEIDDMQIDNIYQRM"
     gene            complement(5771..6556)
                     /gene="lgt"
                     /locus_tag="PKL42_05210"
     CDS             complement(5771..6556)
                     /gene="lgt"
                     /locus_tag="PKL42_05210"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNU66507.1"
                     /translation="MLVHPQFDPVAIHIGTFGIHWYGLMYLTAFLAFFGLGKWQIQHR
                     TWHGWTTSMLDDALFFGALGVVVGGRLGYVLFYQPGHFVENPLDIFKTWQGGMSFHGG
                     FIGVLVAMWVFARKYQLKWLSIMDFVAPLVPLGLGAGRMGNFINGELWGRPTDLAWGM
                     VFPQADALARHPSQLYQFAIEGIIMFAVLWWFSSKPRPVGAISALFLIIYGTGRFLVE
                     YAREPDSYLGLLSLGFSMGQWLSLPMIAIGIWMWRRAVQQKFS"
     gene            complement(6833..7777)
                     /gene="rssA"
                     /locus_tag="PKL42_05215"
     CDS             complement(6833..7777)
                     /gene="rssA"
                     /locus_tag="PKL42_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HNU66508.1"
                     /translation="MVVSKNKMKIGLALGGGSARGWAHIGVLNALTEQGIVPDIVCGT
                     SIGALVGASYASNNFHQLEQWVCSLTKFELVKFFEINTSMNGFVDTARLHSFLNACVA
                     SDSAQIEALTKRFAAVATELDTGREVWLTKGAVLEAVWSSISLPGLFPAIKYHDKWLV
                     DGGLVNPVPVSVCRALGADVVIAVNLNGDIVGKHFQKPHTVDHAPTAAKSSENNGMVQ
                     KITDLVSDYTSSMFSNNNEDKEQAPSLFEAIAGSINITQDRITRSRMAGDPPDILLSP
                     KLSHIGLLEFYRANEAIIEGKNCVARMLPEIHYTLKLK"
     gene            7956..9806
                     /gene="ilvD"
                     /locus_tag="PKL42_05220"
     CDS             7956..9806
                     /gene="ilvD"
                     /locus_tag="PKL42_05220"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNU66509.1"
                     /translation="MPVYRSRTTTHGRNMAGARALWRATGMKDGDFDKPIIAVCNSFT
                     QFVPGHVHLKDLGQLVAREIEKAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRDLIAD
                     SVEYMVNAHCADALVCISNCDKITPGMLMAALRLNIPVVFVSGGPMEAGKVNWQGEML
                     KLDLVDAMVAAADSKVSDAKSEAIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGN
                     GSALATHGARKELFLKAGRLIVEITKRHYEQDDYSVLPRSIANMKAFENAMSLDVAMG
                     GSTNTVLHLLAAAHEAGVDFTMSDIDHISRKVPCVCKVAPATQKYHMEDVHHAGGVMG
                     ILGELDRAGVIHRDTPTVHSATLGEALDKWDIKVTQDEDVKKFYRAAPAGIVTTIAFS
                     QSMLYPTLDDDRAEGCIRDKAHAYSQDGGLAVLYGNIAQDGCIVKTAGVDDSILKFTG
                     RARIFESQDAAVEGILADKIVAGDVVVIRYEGPRGGPGMQEMLYPTSYLKSKDLGKAC
                     ALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVEENDTIEIDIPNRTIHLAVSDEELS
                     HRRAKMEAKGKDAWKPVNRERTVSPALRAYAAMSTSAARGAVRDVSQIER"
     gene            9885..10787
                     /locus_tag="PKL42_05225"
     CDS             9885..10787
                     /locus_tag="PKL42_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HNU66510.1"
                     /translation="MSESNIDFMIQPQLKFSQDESGLEYIEIENRLATAKIALQGGHV
                     MTWQPKSQDQPVLWLSSNARFIKGRSIRGGIPICWPWFGAHPTDSALCPHGFARVIPW
                     QLVDADTLKNGATRLVLQMMETAESRRQLSYPHLLAMTITIGETLKIDLATTNKASHP
                     FVVGEAFHTYFKVSDISNVHVKGLEDALYSDKVFAYERRVEKNDVRFNGEFDRVYLNT
                     TADCMIEDTGLNRQIRISKSGSQSTVVWTPWADKAEQMGDMGAPDEWRTMVCVETANA
                     LENAVVISPNRTHVLSVEYSVETL"
     gene            10791..>10936
                     /locus_tag="PKL42_05230"
     CDS             10791..>10936
                     /locus_tag="PKL42_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HNU66511.1"
                     /translation="MSNRLAEETSPYLLQHADNPVNWYPWSEQALTLAREQDKPIMLS
                     IGYSA"
BASE COUNT         2920 a         2301 c         2703 g         3012 t
ORIGIN      
        1 ccatttttat caagtaacgt gacttggttg tcatcacttc ccatcgcatc ggttgctaaa
       61 ttggcaacta tcaaaggtaa tttttttgcc agtcgcttgg cttctgcgta ttccagtaag
      121 ttttcgctct ctgccgcaaa acctacgcaa aacggtgcgt tgggtaagct ggcgacttca
      181 gcgagaatat ccttattttt tgtgagcgca atggtaagtg tttgctcact ttttttaatt
      241 ttttgtgtgc tggtttgcgc tggtgagtaa tcagcaacag ccgcaacgct gatgaaaata
      301 tctttgggcc caaattttaa gttcgcgata tttttcatca cagcctgata catattttct
      361 gcgctggttg cagaaatgtg tgtcacgtta tggggtgtag caagcgcggt tgcaccgctt
      421 actaaggtaa cttcggcacc catgtctgca gccgcttggg caatggcata gcccattttt
      481 cctgagctta agttggtaat cgcgcgaaca gggtctattt tctcaatggt tgctccagcg
      541 gttattatca cacgcttatt cgctaatagt ttaggcgtaa agtggctatt aatatgcgcg
      601 agtagtgttt cggcctccag catacggcct aaaccagttt caccgcaggc ctgcacgcca
      661 ctttctgggc caagaatggt gacaccatcg gcgctcagtt gtgcaatatt gcgttgcgtt
      721 gccggggctt cccacatttg tttattcatc gcaggtgcaa caagtagtgg gcaatcacgc
      781 gctaagcaaa gggttgagag caaatcatct gcgcgcccat gcacaagttt tgctaaaaaa
      841 tcagcactgg caggcgcaat taaaatagcg tctgcttcac ggcttaactc aatatgcggc
      901 attccgttgc taatgctact atcccacata tccatgaaaa caggcttacc agacaacgct
      961 tgcatggtga ctggcgtaat aaactggcaa gcggccgttg tcatgacaac ttgcacatca
     1021 atttcagctt taactaacag gcgaactaac tctgccgcct tgtaggcagc aataccgcca
     1081 gtaatgccaa ggacgagttt ttttcgtctg atgttggtgt tggtagatat cgtcatggta
     1141 gatttgagct tatgtaatac tctttaaaag atgataatga agctttagtt tatcatgcga
     1201 gattgtgctg tgtgcaactg tcttaaattg aaaagaatca gcagggttta atgatgtgga
     1261 aaaaaattag aatttttata ttactgctcg tttttggggc tgtggtacag caaaccatga
     1321 ttgagcagtc ggatttagat tgggacagga atttttatgt ggcggtatat cctatcaatg
     1381 cggatggcac gactgcggtg gatcactata ttagcacttt gacaaaagag gattttgagc
     1441 cgattgcgga atatttttct gaagaagtga agcgctatgg tttgacaatg cgacgcccgt
     1501 ttcaggtgca acttggcgca aaggtgctac gtcagccgcc tgcaccgcct aaggcagcat
     1561 caattttaga cacgattact tggagcttga agtttagata tttttcttgg aaaaacagcc
     1621 cgcaagttgc agtaaaacct aaaatacgca tgtacttgtt gtattttgat ccaactaaaa
     1681 acgctagatt atcgcattca acggcgctca gtaagggccg tgtaggctat gtgaatttat
     1741 ttggaagtaa agctgctcat cgacaaaata tggttattct tgcgcatgaa atgttgcata
     1801 cactgggtgc aaccgataaa tataacttaa cgactacgtt gccaattttt ccagaaggct
     1861 atgctgaacc caacaaaaca ccacgttacc cgcaagattt tgctgaattg atgggcgggc
     1921 gtgtgccaat cagtgagtcg caggcggaaa ttcctaaaag tttgttacac acactagttg
     1981 gtgaaaatac ggcaaaagaa attggctgga taaagtaaat ctttacaaac tgttctgcaa
     2041 cttgcatttt atgatgcaaa gtatttgatt gaggaattaa gtatggcaat tacagattgg
     2101 cccgccgcag aacgcccacg agaaaaactc atcgaacagg gtgcaggcgc attgtcagat
     2161 gcagagttac tggccatttt cttgcgcgtg ggcgtagtag gaaaaagcgc agtagattta
     2221 gctagagatt tgctcacgca gtttgggagt ctgaatggta tttttgccgc tacagagcat
     2281 gaattaagtc aggtacatgg cataggttcg agcaagtacg tgcagctaca ggcgattttt
     2341 gaaatgagcc gacgtgcctt gagtgaacaa ttgcagcaac gtgatgtgtt gagttcgccc
     2401 caacatgtgc gtgattattt agtattaaaa ttagcgagcc tgaccagaga agtgtttatg
     2461 gtgctttttt tagatacgca aaatagattg ctggccagtg aagagctatt tagtggcacg
     2521 ttaagccaag caagtgttta tccgcgagag gtgattaaac gcgtgttgca ccataatgca
     2581 gcgagtgtga tttttgcgca caatcatccc tcaggcattg cccagcaaag cgcggctgat
     2641 gagttgttaa ctaagcagct gaaagaggcg cttacactgg tggatgtagg tgttttagac
     2701 cattttattg tggcgggaaa tcagacctta tcgtttaaag agcggggttt gttgtaaact
     2761 tatcactaaa atttagcatt cgcataggtg ttgggtatta atcatgaata aatatttcag
     2821 aattttgatt tgggttgcgg ttgcattgtt gggggctttt gcgatatcag gcattgcgtt
     2881 aaaccgagga gaaagcatca atgcgttgtg gctgattact gcggccgtgt gtgtgtatgc
     2941 cattgcatac cgtttttacg cggcttggat tgcggccaaa gtgctggtga ttgatgagac
     3001 gcgtgcaacg cctgcagagc gcttaaataa cggtcgcgac tatattccaa ccaacaaatg
     3061 ggttgtgttt ggacaccatt ttgccgctat tgcagggcct ggcccattgg ttgggccaac
     3121 cttggcggcc cagtttggct atttgccagg cacgctctgg attttaatcg gtgcagtgct
     3181 gggtggtgcc gtgcaagaca tggtgacgtt gtttatttca acgcgccgtg atggtcgtag
     3241 tttagggcaa atggcccgcg atgaaattgg cgcaataggc ggcacggctg cgctggtggg
     3301 tacgttttta atcatgatta ttctcatcgc agtactgggt ttggtggtgg tgaacgccat
     3361 gaaacacagc ccatgggcca cttcaaccgt ggcggctaca attccaatcg ccatgatcgt
     3421 gggcgtttac atgcgcaata ttcgccctgg gcgagtatta gaagcgacaa cccttggtgt
     3481 ggttttactg ctattttcag tttttgcggg tggttggatt gatcaccatg aaaccttacg
     3541 tacgtttttt gatcatgacg gcttgccgct cgcttttgca attattgtgt atggatttgc
     3601 agcggccgta ctgcctgtgt ggctgctgtt agcaccacgc gattatttat cgacctttgt
     3661 aaaattgggc acaattgcat tattagccgt agcgattgtg attttgcggc ctgatatcca
     3721 tatgccagca ttgacgcaat ttactgatgg cactgggccg atatttggtg gaaaactctt
     3781 cccattcgtg tttattacca ttgcttgtgg tgcaatttct ggttttcacg cgctgatttc
     3841 atcgggtact acaccaaaat tacttagtaa cgagcgcgat attcgtatga taggctacgg
     3901 cgcaatgttg cttgaaagct ttgtggcgat tatggcgatg atagccgcaa cggtgttaga
     3961 acctggtatt ttctttgcaa ttaacagccc tgcgggcgtt gtgggtaaag aagccgttga
     4021 tgctgtggca aaaatcaata gttggggcta tgccgtgaca gttgagcaaa tgaaccaact
     4081 tgccaaagac atgggtgaga gcagtttatt tgcgcgcacg ggtggcgcac cgagtttggc
     4141 agttggtatg gccagtattt ttggcagcgc atttggtaag ggcatgttgg cactttggta
     4201 ccattttgcc attatgtttg aagccatttt cattttaacc acacttgatg ctggtacgcg
     4261 agtggggcgt tttatgttgc aagacatgct agggaatttt aataaaaaac ttggtgaaac
     4321 ttcttacatg ccctcggtga ttttaaccag tgcgattgtg gtggcaggct ggggttactt
     4381 tttatatatc ggcgtgatag acccgaatgg cggcgtaaat attttatggc cattattcgg
     4441 tattgcgaac caaatgctgg ctgccattgc cttatgcgta gcaacaggcg ttttggttaa
     4501 atcgggtaaa ttgaaatatg catgggtaac gggtttgcca ttgttttggt tagtgattat
     4561 taccaccaca gccgcctatg aaaaaatatt cagcagtgat attcgggttg gtttttttgc
     4621 cgccgcgaac gatttaagta ccaaattggc cgctggcata ctgccacctg aaaaagcggc
     4681 cgtggcaccg cagttaatat ttaatcagca tctggatgcg tatttaaccc tattttttgt
     4741 ggtggtgttg tgggtggtgt tactggatat gttgcgtttg tgcgtacgat atttagcggg
     4801 taagccggtt gcggcactgt ctgaaacgcc gcgtgaagtg acccaattag agaatgcatg
     4861 ggtgagagat taagcgtaaa tcatgctcaa aaagttaaaa catttatggc acatagtacg
     4921 ccgcctcacg ggcgacgatg cgtatgaggt gtacttaaaa catcacgcag cgtttcatca
     4981 atcggcactt gatgcgccgc cgccgttatc acgcaaagaa ttttttaaaa tttggcaaga
     5041 tagccaatgg aaagacatta agcggtgctg ctagaacatt cactgagtga agaggatgta
     5101 tcgcgcatca tagaaatggc ctgggaagac cgaactgcat ttgaggcaat tgcggttcaa
     5161 tttaacctta atcaagatgg cgtgattaag ctgatgcgta gccaattaaa gccgaattct
     5221 tttaaacgct ggcgcgcacg tacacgtggg caagtgacta agcaccttca cttgcgggtt
     5281 gccggcgtga gcagacacca atcacggcaa cacaataaat tgcatcgctg attgcattta
     5341 cacttacatg cgttgataaa tattatcaat ttgcatgtcg tcaatttcaa aagattctgc
     5401 cgggtctttt aaatcgtatt ctttgttgac tacgcgcgtg ctaatcaagt caaattgtgc
     5461 aggctgatat tgcggcaaaa tgtagttcaa ctctgattct tttttaaaaa gtttatcaat
     5521 taaatttttc atggtcgttt actccaaaat acatcacgct gtgacaatgt tgagtcagat
     5581 tagctgaccc tatacataga taagcaaagc gtatgccaaa aaatttagag ataaacagca
     5641 tgatcggttt ttgtaaccaa ttgaatttta ttgaattatt tgaactgaga aatcatgtac
     5701 ccgcaagatt aacgataaag tgtcatcttg cgaataggga tgacaattct tcgacagatt
     5761 aaattgaaat ttaagaaaac ttttgttgaa ctgctcgtcg ccacatccat atgccgatgg
     5821 caatcatggg gaggcttagc cattggccca tgctaaagcc cagtgacagt aagcctaaat
     5881 aactatctgg ctcgcgtgcg tactccacca aaaatcggcc tgtgccgtaa atgatgagaa
     5941 ataaagccga aatcgcgcca acggggcgtg gcttgcttga aaaccaccac agcaccgcga
     6001 acatgatgat gccttcaatg gcaaattgat acagctgcga aggatgtcgc gcaagtgcat
     6061 ctgcttgcgg aaacaccatt ccccaagcta aatcggtggg tctgccccat aattcgccat
     6121 tgataaagtt gcccattcgc ccggcgccta agcccagcgg cacgagtggc gcgacaaaat
     6181 ccataatgct cagccatttg agttgatatt tgcgcgcaaa aacccacata gcaacgagca
     6241 cacctataaa gccaccatga aagctcatac caccttgcca agttttaaaa atatcaagcg
     6301 gattttcaac aaaatggcct ggctgataaa acaatacata acccaagcgc ccgccaacca
     6361 ccacaccaag tgcgccaaaa aacagggcgt cgtccagcat tgaagtagtc cagccatgcc
     6421 aagtgcgatg ttggatttgc catttgccaa ggccaaaaaa agctaaaaat gccgttaggt
     6481 acatgagccc ataccaatga ataccaaatg tgccgatgtg aattgcaacg gggtcaaact
     6541 gtgggtgaac aagcatggtc ttttgggcta tttttagatg aaagtaggat tttacctaat
     6601 ttaattcgat aaagtgggtt tatcaaaaat ggttttgatg gtgggcattt tccgcttaaa
     6661 attttatatg ctttagcttg aatgttgatg caaaaaaatg aataaaatag gacgaaaaaa
     6721 cacacgagaa aatttttatg ctctgcgagc ctttatttat aaggctcgca gagcatcgaa
     6781 aaaggtttgt cttaaaaaga gcgcattttt agtgatttaa ggcattaaaa aatcacttga
     6841 gttttaatgt gtaatggatt tctggcaaca tgcgtgccac acagtttttg ccttcaataa
     6901 tagcctcatt tgcgcggtaa aattctagta aacctatgtg cgataactta ggggagagca
     6961 aaatgtcggg tggatcgccc gccatacggc tgcgggtaat tctatcttgg gtgatattga
     7021 tggagccggc aatcgcctca aacaagctgg gcgcttgttc tttatcctca ttgttatttg
     7081 aaaacatgga cgacgtataa tcgctcacta aatcggtgat tttttgaacc atgccattgt
     7141 tttcagatga ttttgcagcg gttggcgcat gatcaacggt atgcggtttt tgaaaatgct
     7201 tgcccacaat gtcaccattc aagttgaccg caattacaac atctgccccc aatgctcgac
     7261 acaccgaaac tggcacagga ttcaccaagc cgccatccac cagccacttg tcatgatatt
     7321 taattgccgg aaaaagcccg ggtagtgaaa tggatgacca aacagcttcc agcactgcgc
     7381 ctttggttag ccaaacctct ctgcctgtat ctagctcggt ggctactgcc gcaaagcgct
     7441 tggtgagcgc ctcaatttgc gcgctatcac tggctacgca ggcatttaaa aaactgtgta
     7501 gccgcgcggt gtccacaaaa ccgttcattg aggtattgat ttcaaaaaat ttcaccagct
     7561 caaattttgt gagtgaacac acccattgct ctagctgatg gaagttattt gatgcgtaag
     7621 acgcgccgac tagcgcccca atggacgtac cgcacacaat gtcaggcaca atgccttgct
     7681 cggtcagcgc gttcagtacg ccaatatgcg cccagccgcg cgccgatcca ccacccagcg
     7741 ccaagccaat tttcatttta tttttagata caaccatgcc tgtattgtaa aaccaaacgt
     7801 ttgtgtcagg gcgcactttt aactatgtca cactttaaaa ttaaatctgc tgacaatata
     7861 acgcgttata ttgcatattt ctttggcgag ttagcgtcag ctagtctaaa attacatata
     7921 ataattgttg actcgtttac tacggaattt ttatcatgcc agtttatcgt tcacgtacca
     7981 ccacccacgg ccgcaatatg gcaggcgcac gcgctttatg gcgcgccaca ggcatgaaag
     8041 acggtgattt tgataaaccg attattgcgg tttgcaattc ttttacgcag tttgtgcctg
     8101 gccatgtcca tcttaaagat ttaggccagt tagtcgcgcg tgaaattgaa aaagcggggg
     8161 gtgtggcaaa agaatttaac accatcgcgg tggatgacgg catcgccatg ggtcacggtg
     8221 ggatgttgta tagcctgcct agccgcgatt taattgcaga ttctgtagaa tatatggtga
     8281 atgcgcattg tgctgatgcg ctggtatgta tttcaaattg cgacaaaatt accccaggca
     8341 tgctgatggc ggcattgcgc ttgaatattc cagtggtgtt tgtttctggc gggccgatgg
     8401 aagcgggtaa agtgaactgg caaggtgaaa tgctaaaact agatttggtt gatgcaatgg
     8461 tggccgcggc tgacagtaag gtttcagatg caaaatctga ggcgatagag cgctcggctt
     8521 gcccaacctg cggttcatgc tctggtatgt ttaccgcaaa ctcaatgaac tgcttaaccg
     8581 aggcgttagg cttgagtttg ccgggtaacg gctcggcact cgccacgcac ggcgcacgta
     8641 aagagctttt cttaaaagca gggcgtttga ttgttgaaat caccaagcgc cattacgaac
     8701 aagatgacta ttctgtcttg cctcgttcga ttgcgaatat gaaagctttt gaaaatgcca
     8761 tgagcttgga tgtggccatg ggcggctcaa ccaatacggt attgcacctt ttggccgcag
     8821 cgcatgaggc tggcgttgat ttcaccatga gtgatattga tcatatttca cgcaaagtgc
     8881 cgtgtgtatg caaagtagca ccagccacgc aaaaatatca tatggaagat gtgcaccatg
     8941 cgggtggcgt gatggggatt ttaggtgagc tagaccgcgc tggcgtcatt catcgcgaca
     9001 cgcccaccgt gcatagtgcc actttgggtg aagcattaga taaatgggat attaaagtca
     9061 cccaagatga agacgttaag aaattctacc gcgctgcgcc tgcgggcatt gtcacaacca
     9121 tcgcgttttc gcaaagtatg ctctacccaa ccttagacga tgaccgcgct gaaggttgta
     9181 ttcgagataa agcccacgcg tattcacaag atggtgggtt ggcggtactt tatggcaaca
     9241 ttgctcaaga tggctgtatt gtgaaaacgg ctggcgtcga tgacagtatt cttaaattta
     9301 ccggccgcgc gcgtattttt gaatcgcaag acgctgctgt ggaaggcatt ttggccgata
     9361 agattgtggc tggcgatgtg gttgtgattc gctacgaagg cccacgcggt ggcccgggca
     9421 tgcaagaaat gctctatcca acgagttatt taaagtcaaa agatttaggc aaggcttgcg
     9481 cgttgctcac tgacggccgc ttttcgggtg gaacttctgg tttgagcatt ggccatgctt
     9541 caccagaagc cgctgaaggt ggcgcgattg gattggttga agaaaatgat acgattgaaa
     9601 ttgatattcc aaaccggaca attcatttag ccgtgtcaga cgaagagctc tcgcaccgac
     9661 gcgctaaaat ggaagccaaa ggcaaagacg catggaaacc ggtaaaccgt gagcgtactg
     9721 tttcaccagc gttgcgcgcg tatgcggcca tgagcaccag cgcggcacgc ggcgcggtgc
     9781 gcgatgtttc tcagattgag cgttaacgac tatttacaat gtataatatc gtaaaaaata
     9841 attaaaacaa tagaatataa aaactttgga gaacggttag cgtcatgtca gagagcaata
     9901 tagattttat gatccagcca caattaaaat ttagtcaaga tgaaagtggt ttggaatata
     9961 tcgagattga aaatcgatta gccacagcaa aaatcgccct acagggcggc catgtgatga
    10021 cttggcaacc aaaatctcaa gatcagccag tattatggtt gtcaagtaat gcgcgattca
    10081 ttaaagggcg ctccatacgt ggtggcattc caatttgttg gccttggttt ggcgcacacc
    10141 caaccgatag tgcactttgc ccgcacggtt ttgcaagggt tattccatgg cagcttgtgg
    10201 atgcagatac cctaaaaaat ggcgcaacgc gcttggtgtt gcagatgatg gagacagcag
    10261 aatcaagacg ccagttgtct tatccgcatt tactggcgat gaccatcacc atcggtgaaa
    10321 cgctcaaaat tgatttagca acaacgaaca aagcaagcca cccctttgtg gtaggcgaag
    10381 cttttcatac ctattttaaa gtcagcgata tttcaaatgt acacgttaag ggcttagaag
    10441 atgccttgta ttctgacaaa gtatttgcct atgagcgccg tgttgaaaaa aatgacgttc
    10501 gctttaatgg ggagtttgac cgtgtctatc tcaacaccac agcagactgt atgatagaag
    10561 acacgggttt aaatcgtcag attagaatat caaaatctgg cagccagtct accgtggttt
    10621 ggacgccatg ggccgacaaa gcagaacaaa tgggcgatat gggtgcaccc gatgagtggc
    10681 ggacgatggt atgcgtggaa acggcgaatg cgctagaaaa cgcagtggtt atttcaccca
    10741 acagaacgca tgtgctgtca gtggaatata gcgtagaaac tctataaacg atgtcaaatc
    10801 ggcttgcaga agagacgagt ccttatcttc tgcaacatgc tgacaacccg gttaattggt
    10861 atccgtggag tgagcaagcg ctcactttag cgcgtgagca ggataagcca ataatgctat
    10921 ccattggcta ttcagc
//
LOCUS       DAOQSI010000058        10828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_5321_length_10828_cov_5.422538, whole genome shotgun sequence.
ACCESSION   DAOQSI010000058 DAOQSI010000000
VERSION     DAOQSI010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 10828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10828
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5321_length_10828_cov_5.422538"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="PKL42_05235"
     CDS             complement(<1..779)
                     /locus_tag="PKL42_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HNU66512.1"
                     /translation="MSFQQGLSGLNAASKTLEVIGNNVSNANTVGFKQSRAEFADVYA
                     NSLTGGGASQIGIGTNLANVSQQYTQGNITSTSNTLDIAINGGGFFRMSNNGAVTYSR
                     NGQFQMDKFGYIVDAENKRLTGYTADANGVLSTGAPSEININTADLQPSASTEYKGVV
                     NLDSRKTALPAAGFDPSDPTTYHNSTAVTVFDSLGNEHTVQNFFVKTAPGTWSVFTTA
                     DGISTPALPASTATMTFSGTGVAPTIVPATPTVNFAPTTGA"
     gene            complement(847..1521)
                     /locus_tag="PKL42_05240"
     CDS             complement(847..1521)
                     /locus_tag="PKL42_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HNU66513.1"
                     /translation="MATTVQNTNTASQALLDSVNGKKSKSDSTQEAQDRFMTLLVTQM
                     KNQDPLNPMDNAQVTSQMAQLSTVSGINKLNSTVESLMTSLQMGQSYQAANMIGHGVL
                     VEGKTLTTDGEGGYFGVELPVGADKLSIDIKDTAGNTIRTLDFGAQTAGSVPLIWDGH
                     TNDGTIAANGTYQFDINATVGGQPVQATRLSFAEVKSISNSSNGVTLNLSNLSSVSTS
                     EVKEIF"
     gene            complement(1567..1974)
                     /gene="flgC"
                     /locus_tag="PKL42_05245"
     CDS             complement(1567..1974)
                     /gene="flgC"
                     /locus_tag="PKL42_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HNU66514.1"
                     /translation="MSLFNVFNISGSAMSAQSQRLNAVASNLANADSATSANGTPYKA
                     KQVVFSAIPSASPEAQAVKVTKVVEDTSPAKIVFDPKHPLANEQGYVTMPNVNVTEEM
                     VNMISASRAYQNNVETMNAAKAMLLKTLSIGQS"
     gene            complement(2010..2447)
                     /gene="flgB"
                     /locus_tag="PKL42_05250"
     CDS             complement(2010..2447)
                     /gene="flgB"
                     /locus_tag="PKL42_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HNU66515.1"
                     /translation="MLNKLDAELNFHQTALRVRGQRQELIAANIANADTPNFKARDLD
                     FKTAMQQAMGQNAAMQGLTAAKSIAKTSELHLNALPMVGLQQASGPGEAMYRPISQGS
                     VDGNSVDMDVERNQFVDNGIRYEASLTMLNGQLKKMLAAIQGQ"
     gene            2693..3484
                     /gene="flgA"
                     /locus_tag="PKL42_05255"
     CDS             2693..3484
                     /gene="flgA"
                     /locus_tag="PKL42_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HNU66516.1"
                     /translation="MNTRQLQFKTNAFPRTRVSQYWLCAVFGVGMLFSALSVADEVKV
                     ADVKPAAGLTASKQSLAVVKSKVAEFLTMQTIGYPGEVIVQVGAIDPNLKLAACDDLQ
                     LFLPNGSRAWGKTSVGVQCQAPSKWSIYVQSTGKVVGNYLVAAAPLAQGQVLSDYDVM
                     VVKGDLTHLPAGVFTEQTQAIGRSVQISMTAGTVLRQEMLKVTPVVQQGQTVKLFTSG
                     NGFSISAEGQALAKANEGQVTQVKVASGKVISGIARQGGQVEVAF"
     gene            3629..3946
                     /gene="flgM"
                     /locus_tag="PKL42_05260"
     CDS             3629..3946
                     /gene="flgM"
                     /locus_tag="PKL42_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HNU66517.1"
                     /translation="MNINDSIKSKAVITADKTQLEKPTAKPAQTATGAAAPVAAGESV
                     TLSSLSAQVKSFEAEASAGVFDAEKVQAIKSAISSGQFKVDSEKVADGLIQTVQDLIS
                     AKK"
     gene            4014..4484
                     /locus_tag="PKL42_05265"
     CDS             4014..4484
                     /locus_tag="PKL42_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HNU66518.1"
                     /translation="MNASKPVQISFEQDVTLVNRLLEELTTEQAALVKGDIATVEAII
                     DRKFLLLQQLSTVAKNRYEQLSLHGFQANESGMVAWIKQQANTNMQKAWDQFQKTLTT
                     TKETNRLNGILINKHFSRNQQMLSQLQGKPEAGSMYGKNGQARTSQANQSVLMA"
     gene            complement(5318..6085)
                     /gene="motD"
                     /locus_tag="PKL42_05270"
     CDS             complement(5318..6085)
                     /gene="motD"
                     /locus_tag="PKL42_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotD"
                     /protein_id="HNU66519.1"
                     /translation="MIRRKPIDDDDENPERWLVSYADFITLLFAFFVVMYAISSVNQS
                     KYDQLTSAMGSAFMGQANGGKIILKKESTPENKIHQDSSLIKPLPLSHIYAEKQRKER
                     EAMVKTGLDLSSQLSPLIGAGKVTVVQNNRGVRIDINDSLLFETGSADLSEQAKPLMQ
                     QIAEKLKATPYFMQIEGHTDNAPMHSAAFYSNWELSAVRASSVVRMLNSYGIAENRLS
                     ATGFGATQPISENETAEGRAKNRRVSIMILYETKNPT"
     gene            complement(6262..7002)
                     /locus_tag="PKL42_05275"
     CDS             complement(6262..7002)
                     /locus_tag="PKL42_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HNU66520.1"
                     /translation="MDWNSLIGLAIAVLGILLGQSIEGGHIGSLVQPAAFLIVMCGTF
                     GAVILQTRPKNFIDGVKMLSQVFNMPVDDRQDLAKRINLWSTYARKEGLFMLESYMQK
                     ETEPFIKKGMQLMIDGTPPEKIREIVAIDMHFHEVELRNSAKIWSAAGGYAPTVGILG
                     AVLGLIHVMENLSDPSKLGSGIAVAFVATIYGVGLANLVFLPIANKLKNVIQFEMMRR
                     EMLLNAWVSIAKGDHPNVVAERLLSYMR"
     gene            complement(6984..7718)
                     /locus_tag="PKL42_05280"
     CDS             complement(6984..7718)
                     /locus_tag="PKL42_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HNU66521.1"
                     /translation="MYTAKGKKDQKDELLSTHATLVKKIAYQLKAKLPPNVEIDDLIQ
                     AGMMGLLDAINRYEDTHGAQFETYATQRIRGNMLDELRSADWLPRSIRKNMRDVETAI
                     SQLEQQLGRPPTENEVAKKLQLPLDEYYDMLGDCTGHQLVYYEDFHEAEAGEHFLDRF
                     QTDDKGDPIKAMLEGDFRDALIDAIDSLPDREKMLLGLYYEQELNLKEIGAVMSVSES
                     RVCQLHSQAVARLRATLREKSWTGTV"
     gene            complement(7732..8583)
                     /locus_tag="PKL42_05285"
     CDS             complement(7732..8583)
                     /locus_tag="PKL42_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotR"
                     /protein_id="HNU66522.1"
                     /translation="MRNFQNDQAAGLRRIMSSPKPRVVSILSAGLEATLPRMMTNLAA
                     SIQTQGSQVLLMHASAYSRESQYALNKAASLYDICQNNDSYQNKTALQSAVKVSPYGF
                     SLAKLMQKNQPAHLTSAHVVQTNEQHANQLVKQLATQFDIVLVDASVNDAGKLALPIL
                     NESEIIIQLSRKPESIKQAYALIKLICNQLGRRSFGIIVDDANEEQAQVVFRNIAQVA
                     KRYLQIELEFFGAIPTDEKLSLATKLGRTVIDAFPLAKASAAFTSIAKKLDVQLDHAA
                     PFKQAYR"
     gene            complement(8576..9997)
                     /gene="flhF"
                     /locus_tag="PKL42_05290"
     CDS             complement(8576..9997)
                     /gene="flhF"
                     /locus_tag="PKL42_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HNU66523.1"
                     /translation="MNLKRFFGKNSREALNMVRKTLGENAVIISNRAIEGGNEIIAVQ
                     EDEMNALAMNNYSFNDAPAAKSVVPSSSYTQDASQTMQEIEPITLLEMLNKKNQPNSL
                     SKASHHDFDHLRGNHTPIQTEVRATPKPAQNNHIDLSIAALHHAPAAPNHEMTAILNE
                     MRTMRSSIESQLTTLSWASIQQREPIKAQILSTLLSAGFSAALSRQLAEKLPANLDYG
                     KAITWLKSVLTRNLHSVEDEAEMLGHGGVFALIGPTGVGKTTTVAKLAARYVMKHGTH
                     NLGLITTDAYRIGGHEQLRIYGKILGVMVHTVKDEADLQIALNELKGKHTVLIDTVGV
                     SQRDQMVGEQIAMLSNTDTPIKRLLCLNATSTGETLANVVKAYKGKGLDGCIMTKIDE
                     AATIGNALDVIIREKLKLHYATNGQRVPEDILIANKEQLIQDALNHESLQYLPFKFLA
                     DELPLVMGNTISSANQTAEAHYA"
     gene            complement(9994..>10828)
                     /locus_tag="PKL42_05295"
     CDS             complement(9994..>10828)
                     /locus_tag="PKL42_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HNU66524.1"
                     /translation="ITLKGVEIASGESHFGQYLAIDPGMVTGPLAGQITTDPAFGLPA
                     VWIDNNTRDEAQNMGYTVVDAGTVIATHLNHVISSHAADLLGRQEAQQLLDHVGKESP
                     KLIEDLVPKLLPLSMVQKVLQNLLTEGVHIRDMRTIIETLAEFASQTQDANELTAQVR
                     IKLGRAIVQQLFPSGNEVSVITLDNGLERLLTQAVQSNSTSAAIEPNMAETLARQAES
                     AAKQQEQMGMSPVLLVSAPIRAALAKFLRRSVPHLRVLSHEELPDSKIIRVTNLIGGS
                     A"
BASE COUNT         2818 a         2345 c         2496 g         3169 t
ORIGIN      
        1 ttggcccctg tagttggtgc aaagttaaca gtaggggttg caggcacgat ggtcggcgca
       61 actcctgtgc cagaaaatgt catggtcgcg gttgaggctg gcagcgctgg tgttgaaatg
      121 ccgtcagcgg ttgtaaatac gctccatgtg ccaggcgcag ttttaacaaa aaagttctgc
      181 acggtatgct cgttgcctaa gctgtcaaac accgtcacag cggttgaatt atgataggtt
      241 gttgggtcag acggatcaaa accagcagcc ggtagggcag tttttcttga atccagattc
      301 accacacctt tatattctgt actcgcagaa ggttgcaaat ccgcagtgtt gatgttgatt
      361 tcagatggcg cgccagttga aagtacccca ttggcatctg ccgtataacc cgtcaaacgc
      421 ttattttcgg catctacaat gtaaccgaat ttatccattt gaaattgacc attacgtgaa
      481 taggtcaccg caccattgtt actcatgcgg aaaaacccac caccgttgat ggcaatatct
      541 aaggtattac ttgtgctggt aatattacct tgagtgtatt gttgtgatac gttggcaaga
      601 ttagtaccaa taccgatttg cgacgcaccg ccgcctgtga gtgagttggc ataaacatcg
      661 gcaaactctg cgcgcgattg tttaaaacca acggtgttgg cgttagaaac gttgttgcca
      721 atcacttcta atgttttaga tgctgcattt aatccactta aaccttgttg aaaactcatg
      781 tgattctcct aatctttaat tcgataaacc gctaaattat gttggtaaaa atatgttaaa
      841 aaaactttaa aagatctctt tgacttcact ggtactcact gagcttaaat tgcttaaatt
      901 caaggtcacg ccattgctgc tgtttgatat gctttttact tcagcaaagc tcaggcgagt
      961 cgcttgtaca ggttgcccgc caacggtagc gttaatgtca aattgatagg tgccattggc
     1021 tgcaatcgtg ccgtcattgg tatggccatc ccaaatcagc ggcactgagc cagccgtttg
     1081 cgcgccaaaa tccaatgttc tgatcgtgtt gccagcggta tcttttatgt cgattgataa
     1141 cttatcggcg ccaacaggta gttcaacacc aaagtaaccc ccctcaccat cggttgtgag
     1201 ggttttacct tctactaaca cgccgtggcc gatcatgttg gccgcttgat atgattgccc
     1261 catctgtagg ctcgtcatta gcgattcaac ggtgctgtta agtttgttga tgccgctcac
     1321 cgtcgaaagt tgtgccatct ggcttgttac ctgcgcgtta tccatggggt tcaacgggtc
     1381 ttgatttttc atctgcgtaa ctagcaaagt cataaagcgg tcttgagcct cttgtgtaga
     1441 gtcggattta gactttttgc cattgacact atctaatagc gcttgtgaag cagtgttggt
     1501 gttttgtacg gtggtggcca tgagagtcct ttatttgtag tgactaaatt taacgttaaa
     1561 atgcagttaa gattgaccaa tgctcagcgt ttttagcaac atggctttgg ctgcattcat
     1621 ggtctctacg ttgttttgat atgcgcgtga ggctgaaatc atattcacca tttcttctgt
     1681 tacattcaca tttggcatgg tgacgtagcc ttgttcgttg gctagcggat gttttggatc
     1741 aaacactatt ttggcaggtg aggtatcttc aacaacttta gtgactttta ccgcttgtgc
     1801 ttcagggctg gcgcttggta tggcactaaa caccacttgc tttgctttgt aaggcgtacc
     1861 attggcgctg gtggcgctat cggcattggc caaattactg gcaaccgcat tgaggcgttg
     1921 tgactgtgcg ctcatggcag aacctgaaat attaaaaacg ttaaaaagtg acatggtgac
     1981 ctcctgttat tggtctagat tcataatttt tactggccct gtatagcggc cagcattttt
     2041 ttaagttggc cattaagcat ggttaaactg gcctcgtagc gaatgccgtt atcaacaaac
     2101 tggttacgtt caacatccat atcaacgctg ttgccatcaa cactgccttg cgaaatgggc
     2161 cgatacatcg cctcaccagg cccacttgct tgctgtaacc ccaccattgg cagtgcattc
     2221 agatgtagct cagaggtttt tgcgatgctt tttgccgccg ttaatccttg catcgcagcg
     2281 ttctgcccca tggcctgttg catggctgtt ttaaaatcta aatcgcgcgc tttaaagttt
     2341 ggcgtatcag cattggcaat attggcggca atcagctctt ggcgctgtcc acgcactctt
     2401 agcgcagttt ggtgaaaatt taattcagca tctaatttgt ttaacatcat tggcatcttt
     2461 tctcaaaagg tactgttaaa ccttaagttg aaattaaaaa ggttgaaatt taaagggttg
     2521 aactttgcaa actcaaccat aaaattttcc tttatctatg tgtgccagtt tatagaagat
     2581 gccaagtttt aatcgttgaa gtaaaagggg aaaaaccgcc ttattcttga ttttgttata
     2641 gacgtgctta cgtaaaatgg tcaccatcga atcaatgtag aagaggccta ttgtgaatac
     2701 gcgccaattg cagtttaaaa ccaacgcttt tccacgcaca cgcgttagcc aatattggct
     2761 ttgtgccgtt tttggtgtgg ggatgctgtt ttctgcactg agtgttgcag atgaagttaa
     2821 agtggctgat gtaaaaccag cggctggttt aaccgcatca aaacaaagtt tggcggttgt
     2881 aaaatccaaa gtggctgaat ttttaaccat gcaaaccata ggctacccgg gtgaagtcat
     2941 tgttcaggtg ggtgcgattg accccaattt aaaactggcc gcttgtgatg atctacagct
     3001 atttttgccc aatggcagtc gtgcctgggg caaaaccagt gtgggcgtgc agtgccaagc
     3061 gccaagtaaa tggagcattt atgtgcagag cacgggaaaa gttgtgggca attatttagt
     3121 ggctgcggcg cccttggcgc agggtcaagt tttatcagat tatgacgtaa tggtagtgaa
     3181 gggcgattta acgcatttgc cagctggcgt gtttacggag caaacgcaag ccattggccg
     3241 cagtgtacaa atatccatga ccgctggcac cgtgcttagg caagaaatgc tcaaagtgac
     3301 gcctgtcgtg cagcagggtc aaacagtcaa gctatttaca tcaggcaatg gcttttcaat
     3361 ctcggctgaa ggtcaagcac ttgcaaaagc caatgaaggc caagtgacgc aggtgaaagt
     3421 ggcaagcggt aaagtgattt caggcattgc gcggcaaggc gggcaagttg aagtggcgtt
     3481 ttaatgatct ttataagata cgttttagct aaatgaaaag accataaaaa aatattacaa
     3541 accattaaag ttcgcttaaa ctatgccgtt aagaacacta tggataccaa agaattggta
     3601 tttagaatga aaaataagga tcaatgccat gaacattaat gactcaatta aaagcaaagc
     3661 ggttattact gctgataaaa cacagcttga aaagcctacc gccaaacctg cacaaactgc
     3721 tacaggtgca gccgcgccag tcgctgcggg tgagagcgtt accctatcat cactatcagc
     3781 acaggtgaaa tcatttgaag cggaagcatc tgctggcgtg tttgacgcag aaaaagtaca
     3841 agcgattaaa tcagcaatat cgagcggcca atttaaagtg gactctgaaa aagtggctga
     3901 cggtttaatc caaacggtgc aagatttaat ttcagccaaa aaataatgtg taccttaagt
     3961 attggtttaa gcatcgtatt attttaggca ttatttaaga aacggtgaaa aaaatgaacg
     4021 cctcaaaacc tgtgcaaatt tcgtttgaac aagacgtaac cttagtcaat cggctgctag
     4081 aagagctcac cacagagcaa gccgctttgg taaaaggcga tattgcaacg gtagaagcga
     4141 ttattgaccg caaattttta ctgctgcaac aacttagcac ggtagccaaa aaccgctacg
     4201 agcaattgtc tttgcatggc tttcaggcca atgaatctgg catggtggct tggattaaac
     4261 agcaagcgaa tacgaacatg caaaaggcgt gggatcagtt tcaaaaaacc ttgaccacca
     4321 ccaaagaaac caaccgatta aatggcattt taatcaacaa acattttagc cgtaatcagc
     4381 aaatgttatc gcaactgcaa ggcaaacctg aagccggtag catgtacggc aaaaacggcc
     4441 aagccagaac atcacaagca aaccaatctg tattaatggc gtagttcaat ttgcacactt
     4501 gcccgtgcaa aaaatgcgcc atttattttg cagaaaaaat cacttttttt cacaaaaaat
     4561 tgcatcaacc tttttgactg ccctgtgagc ctttatttat aaggctcgca gggcattaat
     4621 tttttctcga gctttaaacg tgcaatttaa ctgcgttaaa ttgccttaaa ttggcatggc
     4681 gtattttttg taatttatta cgctgaatgt gtgaaatatc ttaaggcgaa atatgcaagc
     4741 tatttcaccc tacatgctag gcgtaccgtt tcaatgatgg gttagcacgc attgactcga
     4801 ccaccacaac cgaaagcaag tattagtacc actaagatta taaaaaacca gaaaaaccaa
     4861 gtgatgagca actgctcacg ataaagtaaa gttttacgac gctttacata attcttaaac
     4921 gaatcagttt ttacgccatg tatccattgc taatcaccta accaagccat gtacaagcga
     4981 actggtaagc ttcgattttc agggttaaat tttcgaaggt taaaacaagc caatagaatc
     5041 gcgataatgg cagctaataa aaacaaccat atatttctcg gaagaagagc tcagtccata
     5101 agttgctaac tgcaattgag cggatgcttg agtccgttta gtaatattga aaatattgat
     5161 gtgagcatat tggtttgctt tttaaatctt aagcttatgt gttgtttttc acacatcata
     5221 ataaatcaaa agtacggacg caattaaatc tttaactcac caaaactaat atgtataaaa
     5281 agagtagtta tgtttttgcc aaaagcacgt agctgattta agttgggttt tttgtttcgt
     5341 acaaaatcat aatggagacg cggcgatttt tggcgcggcc ttcggcggtt tcattttcac
     5401 taatgggttg cgttgcgcca aagccagtgg cgctgagtct gttttcggca atgccataac
     5461 tattgagcat gcgtactacg ctgctggcgc gcacggcaga gagttcccaa tttgagtaaa
     5521 acgccgcgct gtgcatgggt gcgttgtcgg tatggccttc aatttgcata aagtagggcg
     5581 tggctttgag tttttcggca atttgctgca tgagtggttt ggcctgctct gaaaggtctg
     5641 ccgagccggt ttcaaacagt agagagtcgt taatatcaat gcgcacaccg cgattattct
     5701 gcaccactgt cactttgcca gcgccaatca atggcgagag ttggcttgat agatccagcc
     5761 ccgtttttac catggcctca cgttctttgc gctgtttttc agcataaata tgtgaaagcg
     5821 gtaatggctt aatgagtgag ctgtcttgat ggattttgtt ttcaggagtg gattcttttt
     5881 tcagaattat tttgccgcca ttcgcttgcc ccataaatgc tgaacccatg gctgaggtga
     5941 gttgatcgta tttactttgg ttaaccgatg aaatggcgta catcaccaca aaaaaggcaa
     6001 acagcagggt aataaaatcc gcgtatgaaa ccagccagcg ttcggggttt tcatcatcgt
     6061 catctatcgg ctttcttctg atcattttag tgggctatcg ctataaaaaa ttagtaatca
     6121 atgtttgtat tgggtgtatt taaaaacagc ttatttgcta cttaaaactc ccgaggattt
     6181 ttttttgccc tgcgagcctt tatttacaag gctcgcaggg catcgaaaaa ggttgtatat
     6241 aaattaaggc ctttttaacc gttatcgcat gtatgaaagt aagcgttctg ctaccacgtt
     6301 tggatggtcg cctttggcta ttgaaaccca agcattaagt agcatttcac gacgcatcat
     6361 ttcaaactga atcacgtttt taagtttatt ggcaatcggt aaaaacacta aattggccag
     6421 ccctacacca taaatcgttg ccacaaacgc cactgcaata ccactgccca atttgctggg
     6481 gtcagataga ttttccatca catgaattaa gcccaaaacc gcgcccaaaa tgcccacggt
     6541 gggcgcgtaa ccaccagcgg cagaccagat tttggccgag ttgcgtaatt caacctcatg
     6601 aaaatgcatg tcaatggcga caatttcacg aattttttcc ggtggcgtgc cgtcaatcat
     6661 gagctgcatg ccttttttaa tgaaaggctc cgtttctttt tgcatataag actccagcat
     6721 gaacaggcct tctttgcgcg cataggtgct ccagaggttg atgcgcttgg ctaggtcttg
     6781 cctgtcatcg actggcatgt tgaatacttg cgagagcatt ttgacgccgt cgataaagtt
     6841 tttaggccgt gtttgtagaa tcactgcacc aaaggtgccg cacatcacaa ttaaaaatgc
     6901 ggcaggctga acgagcgagc caatgtggcc gccttcaatc gattggccta ataaaatgcc
     6961 taaaacggca atcgctaggc caattaaact gttccagtcc atgatttctc tcttagtgtt
     7021 gcgcgcagcc tagccacggc ttggctatgc aattggcaca cgcgggattc tgaaacactc
     7081 atgactgcgc caatttcttt taagttaagc tcttgttcgt aatacaggcc aaggagcatt
     7141 ttttcgcggt ctggcagtga gtcaatggcg tcaattaaag cgtctctaaa gtcgccttcc
     7201 agcatggctt tgatggggtc acctttgtca tcggtttgaa aacggtctaa aaagtgttca
     7261 cccgcttctg cctcgtgaaa atcttcgtaa tacaccagtt ggtggccggt gcagtcgccc
     7321 agcatgtcat agtattcatc cagcggcagc tgcagttttt tggcaacttc attttctgtc
     7381 ggcgggcggc ctagctgttg ctcaagctgg ctaatggctg tttcaacatc acgcatattt
     7441 ttgcgaatgc ttctgggtag ccagtcggcg ctacgaagct catccagcat attgccgcga
     7501 attcgctgtg tggcgtaggt ttcaaactgc gcgccgtgcg tgtcttcata acggttaatt
     7561 gcgtcgagta agcccatcat gccggcttgt atcaaatcat caatttcaac attgggcggt
     7621 aattttgcct tgagctgata ggcgattttt ttgaccagcg tcgcgtgcgt gctgagcaac
     7681 tcgtcttttt ggtctttttt gccttttgcg gtgtacatat gaatcacaat cttatcgata
     7741 ggcttgttta aagggtgctg catggtccaa ctgaacatct aattttttgg cgatgcttgt
     7801 aaatgcagcg gaagcttttg ctaacggaaa agcgtcaatc acggttctgc caagttttgt
     7861 ggcaaggctt aatttttcgt ccgtgggaat tgcgccaaaa aattccagct caatttgcaa
     7921 gtagcgcttg gccacttgcg caatattgcg aaacacaacc tgggcttgtt cttcattggc
     7981 atcatcaaca ataatgccaa agctacggcg accaagctgg ttgcaaatca gtttaatcag
     8041 cgcgtacgcc tgtttaattg attcaggttt tcttgaaagc tgaatgatga tttcgctctc
     8101 gtttaaaata ggcagcgcta attttccggc atcattcaca gaagcatcca ccaaaacgat
     8161 gtcaaattgc gttgccaatt gttttactaa ttggttggca tgttgttcat tggtttgaac
     8221 cacgtgagct gaagttaagt gcgctggctg gtttttttgc atcagcttgg ctaatgaaaa
     8281 cccatatggc gacactttca cagcactttg taatgcagtt ttgttttgat aagaatcatt
     8341 attttggcaa atatcataaa gtgaagccgc tttgttgagc gcatattgcg actcgcgcga
     8401 gtacgcagag gcgtgcataa gcagcacttg gctgccttgc gtttgaattg aggccgctag
     8461 attggtcatc atgcgcggca atgttgcctc aagccctgca gatagtatgg acaccacccg
     8521 tggtttgggt gatgacataa tccttcttaa gccagcggct tggtcatttt gaaagttacg
     8581 cataatgggc ctccgcagtt tgatttgcgc tgctaatggt gttgcccatc accagcggca
     8641 attcatcggc taaaaactta aagggcaagt actgcaagct ttcatgatta agcgcgtctt
     8701 gaatcaactg ctctttattg gcgattaaaa tatcttctgg cacgcgttgg ccgttggtgg
     8761 cgtaatgaag tttgagtttt tcacgaataa tcacatcaag cgcattgcca atggtggcgg
     8821 cttcatcaat ttttgtcatg atgcagccat ctaagccttt gcctttatag gcttttacca
     8881 cgttggccaa agtttcacct gtgctggtgg cgtttaggca aagtaagcgt ttaatcggcg
     8941 tatccgtgtt cgataacatg gcaatttgct cacccaccat ttggtcacgc tggctcacgc
     9001 cgacggtgtc aattaaaacc gtgtgcttgc ctttgagttc atttaaggca atttgcaagt
     9061 cagcctcatc ttttacggta tgcaccatta cgcctaatat ttttccgtaa atacgcagtt
     9121 gttcatggcc gccaatacgg taagcatctg tagtgattaa acccagatta tgcgtgccgt
     9181 gcttcattac gtagcgtgct gccaatttgg ccacagtcgt ggttttaccg acacctgtgg
     9241 ggccaattag tgcaaatacg ccgccgtgcc ccagcatttc tgcttcatct tcaacagagt
     9301 gtaagtttct tgtgagtaca gattttagcc aagtaatcgc tttaccgtaa tcaagattcg
     9361 ctggtagttt ttccgctaat tgtcttgaaa gtgcagcact aaagccagca gataacagcg
     9421 tactcaaaat ttgtgctttg ataggctcac gttgctgaat gctcgcccat gacaatgtgg
     9481 tgagttggga ttcaattgag ctacgcatgg tgcgcatttc attcagaatg gccgtcattt
     9541 catgattggg cgcagcaggc gcatggtgca gcgccgcaat gctcaaatca atgtggttgt
     9601 tttgcgcagg tttaggcgta gcccgcactt cagtttgaat tggcgtgtga ttgcctctca
     9661 aatggtcaaa gtcatgatga ctcgccttgc tcaagctatt aggctgattt tttttattca
     9721 gcatttcaag caaggtaatc ggttcaatct cttgcatggt ttggctagca tcttgtgtgt
     9781 aagacgatga tggcaccaca ctttttgcag caggggcgtc attaaagctg taattgttca
     9841 tggcgagcgc gttcatttca tcttcttgaa cggcgataat ttcattgccg ccttcaatcg
     9901 cacggtttga gataatcacg gcattttcgc ctagtgtttt gcgcaccatg ttgagtgcct
     9961 cacgcgagtt tttgccaaag aatcgtttta agttcatgct gaacctccaa taagatttgt
    10021 aacgcgaata attttagaat caggtaattc ttcatgtgaa agcacacgaa gatgtggcac
    10081 agagcggcgt aagaattttg ccaatgccgc gcgaataggt gccgaaacca acagcactgg
    10141 cgacatgccc atttgttctt gctgttttgc agcagattct gcttggcgtg caagcgtttc
    10201 agccatgttg ggctcaatag ctgcactagt gctgttagac tgcaccgctt gggtaagtaa
    10261 gcgttcaaga ccattgtcta atgtaattac cgacacttca ttaccactag ggaataactg
    10321 ctgcacaatg gcgcgcccaa gtttaatgcg gacttgtgcg gtgagttcgt tagcatcttg
    10381 agtttggctg gcaaactctg ccaatgtttc aataatggtg cgcatatcac gaatatgtac
    10441 gccttcagta agtaggtttt gcaatacttt ttgcaccatc gataacggca agagttttgg
    10501 cacaaggtct tctatcagtt taggagactc ttttccaaca tggtctaata attgttgggc
    10561 ttcttggcgg cctaataagt cagcggcgtg gctggaaatc acatggttta aatgggtggc
    10621 aatcacagtg ccagcatcca caacggtgta acccatgttt tgtgcttcgt cgcgcgtgtt
    10681 gttgtcaatc catactgctg gtaggccaaa agctgggtcg gtagtgattt gccccgctaa
    10741 cgggccagtc accatgccag gatcaatggc taaatactgg ccaaagtgtg attcaccgct
    10801 ggcaatctcc acgcctttaa gcgtaatg
//
LOCUS       DAOQSI010000059        10622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_5499_length_10622_cov_4.472887, whole genome shotgun sequence.
ACCESSION   DAOQSI010000059 DAOQSI010000000
VERSION     DAOQSI010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 10622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10622
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5499_length_10622_cov_4.472887"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PKL42_05300"
     CDS             complement(<1..653)
                     /locus_tag="PKL42_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNU66525.1"
                     /translation="MKKNIINLAVATTLGLAAFAASAEDMYRGSWYLVPGATYMNTDS
                     DLDANNGGGAFLKLGKELSPSWDIQGGLGYNRASEDLNLGIGEGGRYKQTTLGLDALY
                     MFSRENFRPFLLAGLGAARNNVDYSIPGYDVDGKKTSWLANVGLGAQYLFNDSFGLQA
                     DLRHQWSRAKVKVNGPGINDSDTDTMGNTLFNLGGIFRFGAPAPAPVAAVEPEPAPVA
                     "
     gene            complement(812..1522)
                     /locus_tag="PKL42_05305"
     CDS             complement(812..1522)
                     /locus_tag="PKL42_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(5-formylfuran-3-yl)methyl phosphate synthase"
                     /protein_id="HNU66526.1"
                     /translation="MQLLISVTSVQEAQMALAHQVEILDLKDPNTGALGALPFDVITE
                     VVQYVKNQTQNATPTISATIGDVPMLPEVLVEKVERLQATGVDYIKIGFFAADDYQPC
                     LNALQPIIQAGAKIIAVLFAENTYSATLIESIQRAGFVGVMLDTANKNGQTLFNYYST
                     LALEEFAEKVIMNDMMLGFAGSLKAQDVVKLKMFNPTYLGFRGGVCDNFLRQASLNAE
                     KIRLVQNAMIVCCIKPTI"
     gene            complement(1573..2148)
                     /locus_tag="PKL42_05310"
     CDS             complement(1573..2148)
                     /locus_tag="PKL42_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF447 family protein"
                     /protein_id="HNU66527.1"
                     /translation="MIYEVIVSTINAEGQPHVAPFGVRYEGDLVVISPYKPSTTLANI
                     LATQHAVLNVVDDVCVFAGALTKQQDLSLVATEIVNGFRLKNVLLHKELKLQTVEDDV
                     QRPQLFLQVMHEQYHQSFQGFNRAQAAVIELAVLVSRLHMLPKEKVLSEMAYLQIAID
                     KTAGERELQAWCWLLDQVTNFYSEQIGDNLA"
     gene            complement(2145..3569)
                     /locus_tag="PKL42_05315"
     CDS             complement(2145..3569)
                     /locus_tag="PKL42_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6513 domain-containing protein"
                     /protein_id="HNU66528.1"
                     /translation="MPENLLFLTGKLAEKSLHKVLKEVQSNPNTPAFKYRVEQIGVSV
                     AALMTPEMVARRLKTTGDANKMILPGLCQGDLSQLHRQFGIPAERGPNDLKDLPQYFG
                     QQGIAPDLSQYSTQIFAEIVDAPDLSIEQILAKAQRFQAQGANVIDLGCLPNRPFLHL
                     TEAIYALKSAGFKVSVDSINPEELLAAGKAGADYLLSLTEKTLWIADEVSSTPILIPA
                     KQGSLVSLYRAIEHCLKQNRAFIADPILDPIHFGLTDSIVRYQRLRKKYPDIAILMGV
                     GNLTELTDADTTGINAILFGIISELNINAVLATSVSPHAINAIAEADIARRVMHAAKQ
                     DNRLPRGYSDGLLGLHDRRPFPYHADEIAELAVQIKDPSFRVMVSEAGVHVYNREGMQ
                     VAVDPFDFYPKLGVENDASHAFYLGVELARAQIAWQLNKRYVQDETLLWGVNSRMGEG
                     LKSSNDSPRQSHRELSLKEKQHKK"
     gene            complement(3562..4140)
                     /locus_tag="PKL42_05320"
     CDS             complement(3562..4140)
                     /locus_tag="PKL42_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HNU66529.1"
                     /translation="MLEQRLAWAITGSGHYLSESLALLAQLKNVDVFLSRAAAEIIKQ
                     YGYQAQLDAMGHKIYQDKTASSVPVELFYEGKYHTLVVAPATSNTIAKMAYGFSDSLV
                     TNLYAQAGKTRVPSIIFACDTVPESEDFSLESVAPRDNIVKVFPRKIDLENVAKLATF
                     EHTKIAESFSCLQEALFTRLAEVHSRVDTKNA"
     gene            complement(4177..5505)
                     /locus_tag="PKL42_05325"
     CDS             complement(4177..5505)
                     /locus_tag="PKL42_05325"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNU66530.1"
                     /translation="MAKNSVAKNVVVIGTQWGDEGKGKIVDWLTDHAQGVVRFQGGHN
                     AGHTLVVGQGDAQRVYKLNLVPSGIVRSGINCYIGNGVVLDAGHLLNEIAALEKDGLE
                     VKSRLKVSSGCPLILSHHIALDTAREAKRAVKIGTTGKGIGPTYEDKVSRRALRVYDL
                     FYPSRFADKLREVMDYHNFVLTNYLGAKAVDFNQQYDASMQHAVALKPMISDISADLY
                     AANARGDNLLFEGAQGTLLDIDHGTYPYVTSSNCIAGQASAGTGVGANMLHYVLGITK
                     AYTTRVGGGPFPSELDIDTAGAPGTQMSVKGQEIGTVTKRKRRCGWFDAAALRRSAMI
                     NGLTGLCITKLDVLDGIKELNICVGYELDGKKIDVLPIGADDVAGCKPIYETVAGWTE
                     NTFGVKTWDGLPKNAQHYLKRLEALCGVKVAIVSTGPERDETIVIEHPFA"
     gene            complement(5544..6701)
                     /locus_tag="PKL42_05330"
     CDS             complement(5544..6701)
                     /locus_tag="PKL42_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNU66531.1"
                     /translation="MRNWLLPEYLEDVLPAEAARIEALRRTLLDLFKVHGYQYVIPPM
                     MEYMESLITGAGHDLDLATFKVVDQLSGRLMGVRADMTPQAARIDAHLLNHQGVTRLC
                     YAGSVLRTKPDGLAQTREPLQVGAELYGHAGVESDIEIQRLLIKALHAIHIDGIHIDF
                     SHVGIFESLVQAGNIDANLEQALYQALQSKDQSAVAELAKGLDGKIKESLIHLTTLNG
                     DRRVLEQAARVLPNTPAIQTALQSLAQVSQALVHLNVSVGFDLSELRGYHYHSGVVFA
                     AYAQRYKGPLALGGRYDEVGVAFGRARPATGFSLDLRGVATALSPATLPKAILAPSEM
                     NVDLAAKIDNLRDSGEIVIQALPDAVNNVIELNCDRELVLCAGEWVIEFIN"
     gene            complement(6720..6905)
                     /locus_tag="PKL42_05335"
     CDS             complement(6720..6905)
                     /locus_tag="PKL42_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HNU66532.1"
                     /translation="MKSTFLLALGLMLVLEGLMPLLLPQAWRETFKRLIEFKDGQLRF
                     VGLMSVVGGLLIIWLSK"
     gene            complement(6935..7807)
                     /gene="hflC"
                     /locus_tag="PKL42_05340"
     CDS             complement(6935..7807)
                     /gene="hflC"
                     /locus_tag="PKL42_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HNU66533.1"
                     /translation="MKNLTNILVTVLVALLFLMTSAYTVDQAEFVVVKRLGEIVSVKK
                     SPGLYFKVPFIDDIKRFDNRIVTLDWEQPAKFITSENKYMLVDSFVKWRIIDPAKYYV
                     SVKEGGETAAEDRLSKVVNAGLRSEFGKRTVREVIAGEREVIMKNLRKSADTEARQIG
                     IEVVDVRLKRVDYSEDISKSVYDRMIAERKRLANQLRSEGSAASEKIRADADKQREVI
                     IAEAYREAQKTKGEGDAKAGEIYNQSYSRNPEFYAFYRSQEAYKNSFKNKSDVMVLDP
                     NSDFFKYMRNPAKK"
     gene            complement(7817..9007)
                     /gene="hflK"
                     /locus_tag="PKL42_05345"
     CDS             complement(7817..9007)
                     /gene="hflK"
                     /locus_tag="PKL42_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HNU66534.1"
                     /translation="MIKFPGWKAQNNEGPPDLDQVMRDLSRKINGLFGSGGNGNQSNG
                     QSRIPKGGNVSVPVLPVLAVLLLIWLASGFYLVDSGSKGVVQRFGKMAVDTTEPGPRW
                     HLPYPIEKVTVVNMEQVRRLEVGYRSSGEGSGIKVKQPKEALMLTEDENIIDLQFAVQ
                     YNLKNATDYLFNNRSTDDAVMSAAETAIREVVGKNKLDDLLQKGIQDTSDRMQAILDS
                     YKTGVKIISVSLQSAQPPEQVQEAFEDVNRANQDNQRQINEGQAYANDVIPKARGTAS
                     RLLAEAAGYKLKIESEAKGNASRFDQILAQYNNAPEVTRQRLFLDAQEQILSSVSKVV
                     IDQKAGSLMYLPLDKLMSADNAAPQQSQSMQSLNPQAAVAPEAAATADRTRDAFRSRD
                     REGR"
     gene            complement(9153..10289)
                     /gene="hflX"
                     /locus_tag="PKL42_05350"
     CDS             complement(9153..10289)
                     /gene="hflX"
                     /locus_tag="PKL42_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNU66535.1"
                     /translation="MKDLFERPSGFEAAILVSVDFGDHDYEESLNELRQLSMTAGLAI
                     RGTIESKRNVPDAKYFIGSGKADELKLMMQASEANIAVFNHDLSPSQQRNLERLLEAR
                     VVDRTGLILDIFSQRAQSHEGKLQVELAQLEHLSTRLVRGWTHLERQKGGIGVRGGPG
                     ETQLELDRRMLRVRVKQLREKLIKLKAQRGMQRRARKRSSVMTVSLVGYTNAGKSTLF
                     NRLTKADIYVADQLFATLDTTTHKIFIPDCGSVVISDTVGFIKHLPHALVEAFGATLE
                     EAAQADLLLHVVDTASSNRDEQIAQVNKVLLEIGAANVPQILIHNQIDRVGLLPEITR
                     DEYGRINTIHVSAKTGEGMDLLRLAMAEHYQFLQRKNTEESAYV"
     gene            complement(10329..10565)
                     /gene="hfq"
                     /locus_tag="PKL42_05355"
     CDS             complement(10329..10565)
                     /gene="hfq"
                     /locus_tag="PKL42_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HNU66536.1"
                     /translation="MNQKGQMLQDPFLNALRKEHVAVSIYLVNGIKLQGQVDSFDQYV
                     ILLKNTVTQMVYKHAISTIVPARMVNIGLSEDNA"
BASE COUNT         2906 a         2596 c         2046 g         3074 t
ORIGIN      
        1 gccactggtg ctggttctgg ctcaacagct gcgactggtg ctggcgctgg tgcgccaaaa
       61 cggaaaatac cgcctaggtt aaacagcgta ttacccatgg tgtctgtgtc actatcatta
      121 atgccagggc cattcacttt caccttagca cgactccatt gatgacgtaa gtcagcttgt
      181 aaaccaaatg aatcattaaa gagatactgc gcacctaaac ctacattggc taaccatgat
      241 gtttttttac catcaacatc atagccagga atactgtaat caacgttgtt gcgggcagca
      301 cccaaaccag ccagcaagaa tggacggaaa ttttcacggc taaacatata caaagcatct
      361 aaacctaaag tggtttgttt gtaacggcca ccttcaccaa taccaagatt taaatcttca
      421 cttgcacggt tgtagcccag tccaccttga atatcccagc ttgggcttaa ctctttacct
      481 aatttaagaa acgcaccacc accattgtta gcgtctaaat cgctatcagt gttcatgtaa
      541 gttgcacctg gtaccaaata ccatgaaccg cggtacatat cttctgctga agcagcaaat
      601 gctgctaaac ctaatgttgt agcaactgct aagttgataa tattcttttt cattcgttta
      661 actcctaatt gatagaccat ctaaaatccg tttacagctt tctactccaa ggattagaag
      721 aaagtgcatt tatacaaact gtatacgcgg atactacgca cactcaacgc ttaaggcaat
      781 gcctgaaagc aattaaattg cgacttagcg cctagattgt tggttttatg caacaaacta
      841 tcatcgcatt ttgcactaat cgaatttttt ctgcatttaa gctggcttgc cgcagaaaat
      901 tatcacagac cccaccccta aaacctaaat atgttggatt aaacatctta agtttcacca
      961 catcttgtgc ttttaatgaa ccagcaaaac ccagcatcat gtcgttcatg atgacttttt
     1021 ctgcaaattc ctcaagcgcc aacgttgagt agtaattaaa caatgtttgt ccgtttttat
     1081 tggcggtatc taacatcaca cccacaaaac cagcacgctg aatagattct attaacgttg
     1141 ccgaataggt attttcagca aataatactg cgatgatttt tgcacctgct tgaataatcg
     1201 gttgaagtgc attcaaacat ggctgatagt catctgccgc aaaaaagccg atttttatat
     1261 aatccacacc agtcgcttgc aggcgctcaa ccttttcaac tagcacctct ggcaacatcg
     1321 gcacatcgcc aatggtcgcg ctgatggttg gtgttgcatt ttgcgtttga tttttcacat
     1381 attgcacaac ctcagtaatc acatcgaatg gcaatgcacc taatgccccc gtgttagggt
     1441 cttttaagtc tagaatttcc acctgatgcg ctagggccat ttgcgcttct tgcacacttg
     1501 tgacactgat tagtaattgc atgatgttta aattgccaat tttaagaatt tttatgccaa
     1561 atatgaaaac ttttatgcga ggttatcgcc gatttgttca gaataaaaat tcgtgacttg
     1621 atctagcaac caacaccatg cctgcaactc tcgctcacct gcagttttgt cgatagcaat
     1681 ttgcaaataa gccatttcag ataatacttt ttctttaggc agcatatgca ggcggctaac
     1741 cagtacggcc aattcaataa cagccgcttg tgcgcgatta aaaccctgaa aagattggtg
     1801 atattgctca tgcatcacct gcaaaaataa ttgcggccgt tgcacatcgt cttcaactgt
     1861 ttgcagtttt aactctttgt gcaataacac atttttcaat ctaaacccgt tgacaatttc
     1921 agtggcaact aacgataaat cttgctgctt tgtgagcgcc ccagcaaata cgcacacgtc
     1981 atccactaca ttcagcacgg catgctgcgt tgctaaaatg ttagcgaggg ttgtagaggg
     2041 tttataaggt gaaatcacaa ccaagtcacc ttcatagcgc acgccaaagg gtgccacatg
     2101 aggctgacct tcagcattga tggtactcac aatgacttca taaatcattt tttgtgctgc
     2161 ttttctttga gtgatagttc gcggtgcgac tgtcttggcg aatcatttga acttttcaaa
     2221 ccctcgccca tgcgtgaatt taccccccac aagagcgttt catcttgcac ataacgttta
     2281 ttgagttgcc aggcaatttg cgcacgggca agttcaaccc ccaaataaaa tgcgtgagaa
     2341 gcgtcatttt ccacgccaag ctttgggtaa aaatcaaaag gatccaccgc aacctgcatg
     2401 ccctcgcggt tataaacatg tacgcccgcc tcgctcacca tcaccctaaa actcgggtct
     2461 ttaatttgca ctgcaagctc ggcaatctcg tctgcatggt agggaaatgg tcgcctatca
     2521 tgcaaaccaa gtaaaccatc gctgtaacca cgtggtagac ggttatcttg tttggcagca
     2581 tgcatcacgc gccgcgcaat gtcagcttca gctatggcat tgattgcatg cgggctcact
     2641 gaagtcgcca acaccgcatt aatattgagt tcactgatga tgccaaacaa aatcgcgtta
     2701 atgcctgtgg tgtcagcgtc ggtgagttct gtaagattgc caacccccat caatattgca
     2761 atatcaggat atttttttcg aagcctctgg tagcgcacta tcgaatcagt caaaccgaag
     2821 tgaatcggat ctaaaattgg atcggcaata aacgcgcgat tttgttttaa acagtgttca
     2881 atcgcgcgat aaagtgacac aaggctaccc tgcttagccg gaatcaatat aggcgtagac
     2941 gacacttcat ccgcaatcca taacgttttt tcagtgaggc ttaataaata atccgcaccg
     3001 gcttttccag ccgcaagaag ttcttccggg ttgattgaat cgacactgac tttaaaccct
     3061 gctgacttta acgcatatat cgcttcagtg agatgcaaaa aaggtcggtt gggcaaacaa
     3121 cccaaatcaa tcacatttgc accttgcgcc tgaaaacgct gcgcttttgc cagaatttgc
     3181 tcgatgctca agtctggcgc atctacaatt tcagcaaaaa tctgcgtgct gtattggctt
     3241 aaatcaggtg caatgccttg ttgcccaaaa tattgcggca aatctttcaa gtcattcgga
     3301 ccacgctcag caggaatacc aaactggcga tgcaattggc ttaaatcacc ttggcaaagg
     3361 ccgggcaaaa tcattttatt ggcatctccc gtcgttttta aacgacgcgc caccatttct
     3421 ggtgtcatca atgcggccac tgaaacaccg atttgctcaa cccgatattt aaaggctggt
     3481 gtgtttggat tagattgcac ttctttgagt actttatgca gacttttttc ggctaattta
     3541 cctgttaaaa aaagtaaatt ttcaggcatt ttttgtatca acccttgagt gcacctctgc
     3601 aagccttgta aataaagcct cctggaggca tgaaaaactc tcggcgattt ttgtgtgctc
     3661 aaatgtggct aattttgcaa cgttttccaa gtcaatttta cgcggaaaaa ctttgacgat
     3721 attgtcgcgt ggtgccacag attccagtga aaaatcttca gattctggca ccgtgtcgca
     3781 ggcaaaaata atacttggca cacgtgtttt tcccgcttgg gcataaagat tggtgactaa
     3841 agaatcagaa aaaccatacg ccatttttgc aatagtgttg gatgtggcag gcgcaaccac
     3901 taaagtgtga tatttacctt cataaaacaa ttccacaggt acactgctgg ccgttttatc
     3961 ttgatagatt ttgtggccca tcgcgtctaa ctgcgcctga tagccatatt gtttgatgat
     4021 ttcagccgca gctctgctca aaaaaacgtc cacatttttc agttgcgcaa gcagcgctaa
     4081 cgattcactt aaataatgcc cagaacctgt tattgcccat gctaagcgtt gttcgagcat
     4141 atttacccag tttagtcgtt gcaatgagcc aaaaaattat gcaaacgggt gctcaatcac
     4201 gatcgtttca tcgcgctcag ggcccgttga aacaattgca actttaacac cacaaagggc
     4261 ttctaaccgt ttaagataat gttgggcatt tttaggtaaa ccatcccaag ttttaacgcc
     4321 aaaagtgttt tctgtccagc ctgcaactgt ttcataaatc ggcttgcaac cagctacatc
     4381 gtctgcacca atcggcagaa catcgatttt tttgccatct aactcgtaac ctacacagat
     4441 attgagttct ttaatgccgt caagcacatc aagtttggta atacataacc ctgttaagcc
     4501 gttaatcatg gcagagcgac gcagtgcggc agcatcaaac cagccacaac ggcgcttgcg
     4561 ttttgttacg gtaccaattt cttggccttt aacagacatt tgcgtgccag gcgcacctgc
     4621 cgtatcaata tctaattcgc ttggaaaagg gccgccgccc acacgggtcg tatacgcttt
     4681 tgtaattcct aatacgtaat gcagcatatt tgcacccacg ccggtgcctg cagaagcttg
     4741 tcctgcaata cagttgctag atgtcacata cggataagta ccgtggtcaa tatctaacag
     4801 cgtgccttga gcgccttcaa acagtaaatt atcaccacgt gcattggccg catataaatc
     4861 agctgaaata tcagaaatca tcggctttag ggcaaccgca tgctgcatgc tcgcatcata
     4921 ttgctgatta aaatcaaccg cttttgcgcc caagtagttt gttagaacaa aattatgata
     4981 gtccattact tcgcgtaatt tatctgcaaa tcgcgaaggg taaaataagt cgtacacacg
     5041 caatgcacgg cgcgacactt tgtcttcata agtggggcca atgcccttac ccgtcgttcc
     5101 gattttcacg gcacgtttag cttcacgcgc agtatccagc gcaatatggt ggcttaaaat
     5161 aagcgggcag ccagagctta cttttaagcg gcttttaact tctagtccat ctttttccag
     5221 cgctgcaatt tcatttaaca aatgacctgc gtctagcacc acgccattgc cgatataaca
     5281 attaatgcca gagcgcacaa ttccagaagg cacgagattg agcttgtaaa cacgttgcgc
     5341 atcaccttgg cctactacca aagtgtgtcc agcattatgc ccgccttgaa aacgcaccac
     5401 accttgcgca tggtcagtca accaatcaac gattttacct ttaccttcat cgccccattg
     5461 cgtaccaatg acgactacat tttttgctac tgaatttttg gccataatat gttacttatt
     5521 ttgtatgaaa attaactatt gatttaattg ataaactcaa tcacccattc gcccgcgcac
     5581 aataccagct ctctgtcaca attgagttct atcacgttat tgacggcatc tggtaatgct
     5641 tgaatgacaa tttcaccact gtctcttaag ttgtcaattt tagcggctaa atcgacattc
     5701 atttctgatg gtgccaggat ggctttaggt agggttgccg gtgacaaagc tgtcgcaaca
     5761 ccgcgcaaat ctagactaaa tccagttgca ggtcgcgcac gcccaaaggc aacgcctact
     5821 tcatcatacc gaccgcccaa agccagcggt cccttgtagc gttgcgcata agctgcaaat
     5881 acaacaccgc tgtggtaatg atagccacgt aattcgctta aatcaaagcc aacggaaacg
     5941 tttaaatgca ctaaggcctg actaacttga gccaaacttt gcaatgcggt ctgaattgca
     6001 ggtgtgttcg gaagcacgcg cgcggcctgt tccagcacgc gcctatcgcc attaagcgtt
     6061 gttaaatgaa taagtgactc tttaatcttg ccatccagcc ctttggctaa ctcagccaca
     6121 gcagattggt ctttactttg gagtgcttga tataaagctt gctcgagatt tgcatcaata
     6181 ttgcctgctt gcaccaagct ttcaaaaata cccacatggc taaaatcgat gtgaatacca
     6241 tcaatatgaa tggcatgtag tgctttaatg agtagccgct gaatttcaat atcgctttca
     6301 acgcctgcat ggccatataa ctctgcgcca acttgcaacg gttctcgcgt ttgggctaaa
     6361 ccatctggtt tagtacgcaa tacactacct gcataacata gccttgtcac accttgatga
     6421 tttaacaaat gcgcatcaat acgtgcagct tgcggcgtca tatcagcacg cacacccatc
     6481 aaccggccgc tgagttgatc aaccacttta aacgtggcca agtctaaatc gtgtcccgcg
     6541 ccggtgatga gtgattccat gtattccatc atcggcggaa ttacgtactg gtagccatgc
     6601 actttaaata aatcgagcaa ggttctacgt agtgcttcta tgcgtgcagc ctcagcaggg
     6661 agcacgtctt ctaggtattc cgggagtaac caattacgca tgtttatcta cttaatactt
     6721 tatttactta accatatgat aagtaatccg cctaccaccg acattaatcc gacaaagcgt
     6781 aattgcccat ctttaaattc tatcaaccgt ttaaaagttt cacgccatgc ttgtggtaac
     6841 aataaaggca tcaatccttc tagcaccagc atcaacccta acgccaataa aaatgtactc
     6901 ttcacttgaa aaatctttga aatttaaacc aagcttattt cttcgcaggg tttcgcatat
     6961 atttaaagaa gtcggaattt ggatctaaca ccatgacatc acttttattc ttgaagctat
     7021 ttttataggc ttcctggcta cggtagaacg cgtagaactc aggattacgg ctatatgatt
     7081 gattgtatat ctcaccagct ttagcatcac cctcaccttt ggttttttgt gcttcacgat
     7141 atgcttcggc aataatcact tcacgctgtt tatctgcatc ggcacgaatt ttctcagatg
     7201 cggctgaacc ttctgagcgc aattggttgg ctaagcgttt acgctcggca atcatacgat
     7261 cataaaccga cttgctgata tcttctgagt aatccacacg tttcaaacgt acatccacca
     7321 cttcaatgcc gatctgacgt gcctcagtat cggcggattt gcgcaagttt ttcataatca
     7381 cttcacgctc gcctgcaatc acttctctga cggtacgttt accaaactca ctacgcaaac
     7441 cagcattcac cactttagaa agcctatctt cagcagcagt ctcaccgccc tctttaactg
     7501 aaacatagta tttagcaggg tcaataatgc gccactttac aaaagaatct accagcatat
     7561 atttattttc actggtaata aatttagctg gctgctccca atctagggtc acaatacggt
     7621 tatcaaaacg cttaatgtca tcaataaaag gcactttaaa atatagcccg ggtgattttt
     7681 ttactgagac aatttcaccc aagcgtttta ctactacaaa ttcagcttga tcaaccgtat
     7741 aagcggaggt catcaaaaac aacagtgcaa ctaaaacagt taccaatata ttcgttaaat
     7801 ttttcatggc gaatccctat cgtccttctc tatcacggct acgaaatgca tctcgcgtac
     7861 gatctgccgt tgcggcagct tcaggcgcca ctgccgcttg tgggtttaaa gactgcattg
     7921 attgagattg ttgtggtgca gcattgtcag cgctcatgag tttatcaagc ggaaggtaca
     7981 tcaggctacc tgctttttga tcaatcacca ctttacttac actagataat atctgctctt
     8041 gtgcatctaa aaacagacgt tgacgagtca cttcaggtgc attgttgtat tgcgccaaga
     8101 tttggtcaaa acggcttgcg ttacccttgg cttcgctttc aattttaagt ttgtaacccg
     8161 ctgcttctgc aagtaatctt gaagccgtac cacgcgcttt tggaatgaca tcattcgcat
     8221 aagcttggcc ttcgttaatt tgacgttgat tgtcctgatt ggcgcggtta acgtcttcaa
     8281 acgcttcttg cacttgctct ggcggttgtg cgctttgtaa agacacactg atgattttga
     8341 cccctgtttt atagctgtct aaaattgcct gcatccggtc tgaagtatct tgaatgcctt
     8401 tttgcagcaa atcatctaat ttatttttac ccacaacctc acgaatagct gtttctgcag
     8461 cagacatcac agcatcatct gttgaacggt tgttaaacaa ataatcggtc gcatttttta
     8521 ggttatattg cacagcaaac tgcaaatcaa tgatgttttc atcttcggtg agcatcaatg
     8581 cttctttagg ttgctttact ttgatgccgc tgccttcacc gctgctacga taacccactt
     8641 ctaaccgacg cacttgctcc atattaacca ctgttacttt ttcaattgga tacggtaggt
     8701 gccagcgagg ccctggctcg gtcgtatcta ctgccatttt accaaagcgc tgtaccacgc
     8761 cctttgagcc agaatccacc aagtaaaagc cgcttgccag ccaaatcagt aacaaaacag
     8821 caagaacagg caatacaggc acactcacat tgccgccttt tggtattctg gattggccgt
     8881 tggattgatt gccattgcca ccgctgccaa ataagccatt gattttacgg cttaaatcgc
     8941 gcatgacttg gtctaggtct gggggacctt cgttattttg tgctttccat cctggaaatt
     9001 taatcattca aaactccata aaaacaaact taatttattt cagtgccaag caatatctac
     9061 acaccaacca acgatattct taattcaaaa ttctaaattc aaaccaatat tctttttcaa
     9121 agtttgttgc gtaaaccaac ctaaatctaa agtcacacgt acgcactttc ttctgtgttt
     9181 tttctctgca aaaattggta gtgttctgcc atcgccaagc gtaataaatc cattccttct
     9241 ccagttttag cagagacatg aatggtgtta attctaccat attcatcgcg cgttatttct
     9301 ggcaatagcc ccacgcgatc aatttgatta tgtatcaaaa tttgcggtac attggccgcg
     9361 ccaatttcta acagtacttt attgacttgt gctatttgct catcacggtt actactcgca
     9421 gtatcgacca catgaagcaa caaatccgcc tgcgccgctt cttccaaagt agcgccgaac
     9481 gcttcaacta gggcatgtgg taaatgttta ataaaaccaa ccgtatctga aataaccact
     9541 gagccacaat ccggtataaa aatcttatgc gtggtggtat ccagtgttgc aaacaactgg
     9601 tctgccacat aaatatctgc ttttgtgagg cgattaaata gcgttgattt gcctgcattg
     9661 gtgtaaccaa ccaacgacac cgtcatgacg cttgaccgct tacgtgcacg tcgctgcatg
     9721 ccacgctgcg ctttaagttt tatgagtttt tcacgcagtt gttttacgcg aacgcgcaac
     9781 atccgacgat cgagttccaa ctgcgtctca cccggaccac cgcgcacccc aataccgcct
     9841 ttttgacgtt cgagatgggt ccagccacgt actaagcgcg tcgataagtg ctcaagttgc
     9901 gctagctcga cctgtagctt accttcatgg ctttgcgcac gttgactgaa aatatcaaga
     9961 atcaagccgg ttctatccac cacgcgggct tcaagcaagc gctcaagatt gcgttgctgg
    10021 gatggtgaca ggtcatggtt gaagacagca atatttgctt cactagcctg catcatcagc
    10081 tttaattcat cggctttgcc cgaaccgata aaatatttag catcaggcac attgcgcttt
    10141 gattctattg tgcctcgaat agccaaacca gccgtcatac tgagttgacg gagttcattt
    10201 aggctttctt cgtaatcatg atcaccaaaa tcaacgctca ccaaaattgc agcttcaaag
    10261 ccacttggcc tttcaaataa atctttcaag gttaattaaa cccaatcttt aaatttattt
    10321 gctaccactt aggcattatc ttcagataag ccaatattga ccatgcgcgc agggacaatc
    10381 gtggaaattg catgtttgta caccatttgc gttactgtgt tcttgagcaa aatgacatac
    10441 tgatcgaatg aatctacctg accttgcaac ttaataccat ttacaaggta gatagaaaca
    10501 gcgacatgct ctttgcgtaa tgcgtttaga aatgggtctt gtaacatttg ccctttttgg
    10561 ttcatgactt tctcctatta cttggagtta aattaaatgg ttatagcttg cgctacagtg
    10621 ta
//
LOCUS       DAOQSI010000060        10457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_5648_length_10457_cov_6.226879, whole genome shotgun sequence.
ACCESSION   DAOQSI010000060 DAOQSI010000000
VERSION     DAOQSI010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 10457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10457
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5648_length_10457_cov_6.226879"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1362)
                     /gene="dnaE"
                     /locus_tag="PKL42_05360"
     CDS             complement(<1..1362)
                     /gene="dnaE"
                     /locus_tag="PKL42_05360"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNU66537.1"
                     /translation="MSNANSFEPSQVLQNAPAFVHLRCHSEYSIVDGIVRIDDYVKQA
                     LADQMPALALTDLNNLFGAIKFYKAARGRGLKPILGCDIWLENSTNRDQPTRLLLLCQ
                     THAGYLLLCQLLSRAYLTNQYRGRTEFKREWFEECGTEGLLLLSGAMAGDIGQACLQD
                     NIALATQLAQDWNDLFPDRFYLEVQRVATDNKVQQEHYISHIRSIAHQLNLPVVATHP
                     IQFTTPDDFRAHEARTCISEGYVLADTRRPKHFTTEQYFKKQAQMAALFADMPEALTN
                     SVEIAKRCNLSLTLGKNYLPNFPTPNNESLDEFLILEAKRGLTKRLEVLYPNEEVRHA
                     KQPAYDARLEFETKVINQMGFAGYFLIVADFIQWAKHNGVPVGPGRGSGAGSVVAYSL
                     GITDLDPLEYNLLFERFLNPDRVSMPDFDIDFCQEGRDRVIDYVKQKYGLDAVSQIAT
                     FGTM"
     gene            complement(1553..6754)
                     /locus_tag="PKL42_05365"
     CDS             complement(1553..6754)
                     /locus_tag="PKL42_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNU66538.1"
                     /translation="MAEVFDAGPLSWVKDEIDRSLKKVQDNFAAVTEKPEEFATLRFT
                     IAHLYQISGALDMVGLEGCKRYCAEIEKLTSKLEKQEIPVTQALMAHLSHAISTLEQY
                     LQDLLNGYPDVPTRLFESIKPLVEAQGEALEITDLFFPDTGFNAPKDLPTSELPEFAV
                     PLLVAEQRTVFQKYLLDWLRNHDSEALSHMREAVVKVQQVQHKSAQKTLWWAAIALME
                     ALSQEKIAALSGAKKLCRRLDQQLRNMAEGDAKTPSQLLRDVLYYVAISDRTTETIAR
                     VKETFELDQVLPLNVTGASGQGNLTTDAERTALAQLSADLPILKDLWTRISENPSSTE
                     DLATFAGKFQHLSNVQQGLNNQAAYALVNAIYAAASALHTDPLKVNEVSLIEIAAALN
                     LLEYISEHYAQLDVQMQAKITTQTARLLAVVDGVPLAPPLEVFKNETKLDASVMEAVA
                     KQVLAALLHAEKAFDAFFRNPTDAALLDDTVKPLQQVSAAFDMLEMPTQKSISDLSLG
                     FVQYFKEHIGYLEQSTFELLAESLSMLGLYAEELPNARAESETALQGAQYRLERHAKA
                     LDIVQNTDVVEAHQELETDITEDNEVTKSTVAPYPIIDGLDELPRITDPDSTIDDVTD
                     EAIDEELQEIFLAEAEEVLINIAQHLKALGVNGRDSEALADLRRAYHTLKGSGRTVGL
                     VGMSKVAWAIEKLLNLIIEHKAFPSPQHLAFIERVTGAFARCVGHLQADKKVTLNPTI
                     FQKEAATYEREFELSLTGEQPKPVKEEVLIGGTRRLSREFFNIFLHEAQQHLATLAEA
                     QSALTLNSTELPTDASCRAAHTLGSNALTAGFKPTGDLARALEYWLDEHRASWTAQHI
                     NLYANVVKALSDGIERIKTLKNPKSSRALIVALGESTAEMQAIAAQQAEAAVLELEKE
                     MQSAGISVDKLKVKVPPAGKVADKKQKRESNKPAIHESVELDTHEEPSADEVVVALDE
                     IAVFGQQSSFGTETEIAEPQLTSSQQASKPNVPVDQELLALFTEEARELVPQVGRDLR
                     GWRNNPHETEYSDSLQRALHTLKGSARMAGLATIGDSVHLMEDRVIHALRNAPTPSDF
                     EEMFAEFDRIGYMLEDLTGGASEKQSVEEVSIKPAAPTRAADRKYQYLRMRADVLDRL
                     INEAGEVSIMRSRMEREMQNFKQSSTDLTDSISRMRAYLRELEIEAETQMQSRMSLLQ
                     EAHEEFDPLEFDRFTRLQELTRMLAESVNDVATIQHGLLLNLDQTDAALQQQNRMNRE
                     LQQGLMGVRMLPFATITERMQRIVRQTARELGKRVEMTIEGEDIEIDRGVLDKIGAPL
                     EHLLRNAVAHGLETTAERRVLGKPEVGQIKLKVSLENDEITLVVTDDGAGIDLEKVKQ
                     KAIEKGLFEANQALSDQALMAVIFEPGFSTADTISQIAGRGVGLDVVRSDIAALSGRI
                     DVSNVAGLGAMFNIYLPVTLSVAQVVMVRAGHKIFAIPSVMVEQVQKLKPADLNQAYA
                     TQKIEWSNRDYPLHYLAKLIGDADTVAELHAYTPMVLMRSGIYRTALHVDEILGNQEV
                     VMKQIGTQLARVPGIIGATVLGDGDIVLVLNAVQLANREALSVGSVKVSSATPAIEQR
                     RRIALVVDDSLTMRKVLSRVLERDDFEVLTANDGMDAVQKLQQITPDIILTDIEMPRM
                     DGFEFSRHVRDNQATKHTPLIVISSRTADKHRNLATEIGVNAFLGKPVQDEDLIAQVN
                     ALLGQNA"
     gene            complement(6858..8612)
                     /locus_tag="PKL42_05370"
     CDS             complement(6858..8612)
                     /locus_tag="PKL42_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNU66539.1"
                     /translation="MALNLAVVKSLPQLLGGFFSKLTSKKASASSSETVGSGLKQLYL
                     QIAAFIIFAALSFWVINQVRVNGPVYNALHDFDTLVADTVPPPLYPVDAFAAYNEAYV
                     ASSNFNNDKRDKALAKAARFESLFRERSAYWQENIENPELKGALLASIKAGENFFLVA
                     KNRFIPSLPLGTVAASAPLSELTAAFEITNQTGNTLREAVASENTKLLESQTNFIQLV
                     LAGLLLLGLTLLALMYFFGKKQATQAMKLTEQLAKDGQENQEAVLQLLDEMGDLSDGD
                     LSVKAQVKDNITGAIADSINYTIDNLRDLVLEINRATEQVTSATEVAQETSTKLLSAA
                     ETQSEQILQTTDAVTDMTRSILQVSNNATQASEVAQRSLEAANQGSQAVQNTIAGMND
                     IRTQIQETSKRIKRLGESSQEISEIVELISDITEQTNILALNAAIQAASAGEAGRGFT
                     VVAEEVQRLAERSSEATKQISAIVKTIQTDTHGAVAAMEKSTEGVVDGARVADAAGQA
                     LTEIESVTVNLARLIQSISTATSAQTESASVVANNMQLIQEITTQTSDGTKLTAEAVG
                     QLTSLAEELRTSVAGFKL"
     gene            complement(8728..9258)
                     /locus_tag="PKL42_05375"
     CDS             complement(8728..9258)
                     /locus_tag="PKL42_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNU66540.1"
                     /translation="MAKTSNLREFQEGILQKLKEATSQGAAESTSRLGVMVGEYRLLI
                     NLTAVTEVLPVPTLQSVPLTQPWFLGVANVRGNLYNITDLLQFIGKPALPKTIHSRIL
                     LLNSLNTTQTALVVTSLVGLRNIESMKVKQKAIIERGLLDLLIKQVYEDENQQTWEEL
                     NIEALVQHQDFIQPTI"
     gene            complement(9310..9675)
                     /locus_tag="PKL42_05380"
     CDS             complement(9310..9675)
                     /locus_tag="PKL42_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNU66541.1"
                     /translation="MAIEKILVVDDSATDRFFLTELLETAGFSVTGADSGEDCLDKVA
                     TNPPDLVLMDVVMPGLTGFQVTRMLTKDPKTTNIPVILCTGKSQATDLAWALKMGAKA
                     CVTKPVVSADLLALIKTLG"
     gene            complement(9677..10057)
                     /locus_tag="PKL42_05385"
     CDS             complement(9677..10057)
                     /locus_tag="PKL42_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNU66542.1"
                     /translation="MVIDDSNTIRRSAEIFLKNSGCKVILAEDGFDALAKIANEHPDI
                     IFVDIMMPRLDGYQTCSLIKRNARYKTTPVIMLSSKDGLFDRARGRMVGSDQYLTKPF
                     TQETLVDAVQTYASLGKNNQEEAD"
BASE COUNT         2742 a         2564 c         2145 g         3006 t
ORIGIN      
        1 catagtaccg aaagtggcaa tttgcgatac ggcatctaac ccatattttt gcttcacata
       61 atcaatgacc cgatcgcggc cttcttggca aaagtcgata tcaaagtcgg gcatggatac
      121 gcggtcagga tttaaaaagc gttcaaacaa taaattgtat tcaagcgggt caaggtcagt
      181 aattcccaaa ctgtaggcca ccactgaacc cgcgcctgaa ccacggcctg gcccaaccgg
      241 tacgccattg tgttttgccc attgaataaa gtctgccaca attaaaaaat agccggcaaa
      301 acccatttga ttgatcactt tggtttcaaa ttcaagcctg gcgtcatacg caggctgctt
      361 ggcatggcgc acttcttcat tcgggtacaa cacttccaat cgcttggtta agcctctttt
      421 cgcttcaaga attaaaaatt cgtccagact ttcgttattc ggtgttggaa agttaggtaa
      481 gtaattttta cctagggtta gcgataaatt acagcgtttg gcaatttcca cactgttggt
      541 gagcgcttca ggcatatcgg caaacaaagc ggccatttgc gcctgctttt taaaatattg
      601 ctccgttgta aaatgcttcg gccgacgcgt gtcggcgagc acatagcctt ctgaaataca
      661 ggttctggct tcatgcgcgc gaaaatcatc gggcgttgta aattgaatgg ggtgcgtggc
      721 caccacaggt aagttgagct ggtgagcgat gctgcgaata tgcgaaatat aatgttcctg
      781 ctgcactttg ttatccgttg caacacgctg cacttcgaga taaaaacggt ctgggaataa
      841 atcattccaa tcttgggcga gttgcgttgc cagcgcaata ttatcctgca aacaggcctg
      901 accaatatcg cccgccatag cccctgagag caatagcaag ccctcagtgc cgcactcttc
      961 aaaccactcg cgcttaaact cggtacggcc gcgatattga ttggtgaggt aagcgcggct
     1021 taataattgg cacagcaata gataaccagc atgcgtttgg cataataaaa gcaagcgagt
     1081 tggttgatcg cgattggtgc tattttctag ccagatatca caacccaaaa tcggttttag
     1141 gcccctaccg cgtgcggctt tataaaactt gatggcacca aataaattat tgaggtcagt
     1201 gagcgccagt gccggcatct ggtcagccag tgcctgtttg acataatcgt caatgcgcac
     1261 aatgccatcc acaatcgaat attcgctgtg gcaacgcaaa tgaacgaatg caggcgcatt
     1321 ttgtaaaact tggctaggtt caaatgagtt tgcgttggac atagcctaaa ttttacctga
     1381 ttgacgcaaa tttagagact aatacttagg ataatttaat agaaaaaata acccaaaaat
     1441 gacatgtttt actacgagag aaaaacaatg ccctggaagc cttataaata aaggcttgca
     1501 gggcagcgta aaaggtttgt gctgaaatag actaaaaaaa tgaaatttta gatcatgcgt
     1561 tttgcccaag caatgcattg acctgcgcaa ttaaatcttc atcttgcacc ggtttaccca
     1621 agaaggcatt cacgccgatt tctgtcgcca aattgcgatg cttatcagcc gtgcgagaac
     1681 taattacaat cagcggtgta tgtttggtgg cttggttgtc acgcacgtgc cttgagaatt
     1741 caaagccatc catacgcggc atttcgatgt ctgtcagaat aatatccggt gtaatttgct
     1801 gtaatttttg caccgcatcc atgccgtcat tggcagtcag cacttcaaag tcatcacgct
     1861 ccaatacgcg tgacagcacc ttacgcatgg taagtgagtc atccaccacc aaggcaattc
     1921 tgcggcgctg ttcaatggca ggtgttgctg aactcacttt gaccgaacct acagacaatg
     1981 cctcacgatt ggccaactgc actgcattga gcactaaaac gatgtcgcca tcacccaata
     2041 ccgtggcgcc aattatgcct ggcacacgcg caagttgggt gccaatttgc ttcatcacca
     2101 cctcttggtt gcccaaaatt tcatccacat gcaacgcggt gcgataaata ccgctacgca
     2161 ttaataccat gggcgtgtaa gcatgaagtt cagccaccgt atcagcatca ccgattagtt
     2221 ttgcaagata atgcagcgga tagtcacgat tcgaccattc aattttttgt gttgcatacg
     2281 cttggtttaa atctgcgggt tttaactttt gcacctgttc caccattact gagggaatcg
     2341 caaaaatctt atggccagcg cgtaccatca ccacttgcgc aaccgacagc gtgacaggca
     2401 ggtagatatt aaacatcgcg ccaagaccag ccacattgct aacatcaatg cggccgctta
     2461 gtgccgcaat atcactgcgc accacatcta aacctacgcc tcggccagca atttgcgaaa
     2521 tcgtatccgc tgttgagaac cctggctcaa aaattactgc catcaaagct tgatcagaca
     2581 atgcttgatt cgcttcaaaa agcccttttt ctatcgcctt ttgtttaact ttttcaagat
     2641 cgatgcctgc gccatcgtca gtgacaacca gcgtaatttc atcattttcc aaacttactt
     2701 taagtttgat ttggccaact tctggcttcc ctaacacgcg gcgttctgcg gtcgtttcta
     2761 aaccatgcgc aactgcgtta cgaagcaaat gctcaagcgg cgcaccgatt ttatcgagca
     2821 cgccacgatc aatctcgatg tcttcacctt caatcgtcat ttcaacgcgc ttgcctaact
     2881 ctctagcagt ttggcgcaca atacgctgca tgcgctcggt gatggttgca aacggcaaca
     2941 tccgtacgcc catcaagccc tgctgcagct cgcgattcat acggttttgt tgttgaaggg
     3001 cggcatctgt ttggtctaaa ttcaataaca gaccgtgttg aatagtggcc acgtcgttca
     3061 cactttcagc cagcatgcgc gtgagttctt gcaaacgggt gaatcggtca aattctagcg
     3121 ggtcaaattc ttcatgcgcc tcttgtaaca aactcatacg cgactgcatt tgtgtttccg
     3181 cttcaatctc caactcgcgc aaataagcac gcatacggct gatgctgtcg gtcaaatcgg
     3241 tggaagattg cttgaaattc tgcatttctc gttccatacg agagcgcata atcgacactt
     3301 cacccgcctc attaatgagt cgatcaagca catcagcacg catacgcaaa tactgatatt
     3361 tgcggtctgc agcgcgtgtg ggcgccgctg gcttaatgga aacttcttca acagattgct
     3421 tttcactagc accaccggtg aggtcttcca acatatagcc aatgcggtca aactcggcaa
     3481 acatttcttc aaaatcactt ggggttggtg cgttccttag cgcgtgaatc acgcgatctt
     3541 ccataagatg cacgctgtca ccgatagtgg caagccccgc catccgcgca cttcctttta
     3601 aggtatgcag cgcacgctgt aatgaatctg aatattctgt ttcatgcgga ttgttacgcc
     3661 atccgcgcaa atcacgtcca acctgtggca caagttcacg cgcttcttcc gtaaataacg
     3721 ctaggagttc ttggtcaact gggacattcg gtttgctggc ctgctgactt gatgtaagtt
     3781 gtggttcggc tatttcagtt tccgtaccaa acgatgactg ttgcccaaat acagcaattt
     3841 catcaagtgc aaccacgact tcatccgcgc ttggttcctc atgtgtatcc agctcaactg
     3901 attcgtgaat agctggttta ttagattccc ttttctgttt cttatctgca actttacctg
     3961 ctggcggcac tttaactttg agtttatcaa ctgaaatgcc ggcagactgc atttcttttt
     4021 caagttccag tacggctgct tccgcctgtt gggccgcaat agcctgcatt tccgcggtgg
     4081 attcacccaa tgccacaatt aatgcgcgtg atgattttgg atttttaagt gttttaatac
     4141 gctcgatgcc atctgataat gctttcacaa cattcgcgta taaattgata tgttgtgccg
     4201 tccagctggc tcgatgttcg tctaaccaat actctaaggc acgtgctaaa tccccagttg
     4261 gcttaaagcc tgcggttaac gcgttgctcc ctaatgtgtg tgctgcacgg cagctggcgt
     4321 ctgttggcaa ctccgttgag tttaaagtca aagcgctttg cgcttcggct aaagtcgcca
     4381 aatgctgctg tgcctcgtgc aaaaagatat taaagaattc gcggcttaat ctgcgcgtgc
     4441 caccaattaa aacttcttct ttgactggtt ttggttgttc gcctgtgagg gataactcaa
     4501 actcgcgctc ataggtagcg gcttcttttt gaaaaatagt tggattgagc gtaacttttt
     4561 tatcagcctg cagatgtccc acacaacgcg caaacgcccc tgtcacgcgc tcaataaacg
     4621 ctaaatgttg cggacttgga aatgccttat gctcaataat taaattaagt agtttttcaa
     4681 ttgcccaggc tactttgctc atacccacta agccaaccgt acgcccactg cccttcagtg
     4741 tatggtatgc acggcgtaaa tcagccagcg cttcactatc gcggccattg acgcctaacg
     4801 cttttaagtg ttgggcaatg ttaatgagca cctcttcagc ttccgccaag aatatttcct
     4861 gtagctcttc atcaatcgct tcatcagtca catcgtcaat cgttgaatcc gggtcggtaa
     4921 ttctcggtaa ctcgtctaaa ccatcgatga ttggataagg tgcaacggtg gatttagtca
     4981 cctcattatc ttctgtaata tctgtttcta attcctgatg cgcctccaca acgtcagtat
     5041 tctgaacaat atccaacgct ttagcatgcc gttctaatcg atattgcgct ccttgcaagg
     5101 ctgtctctga ttcagcgcgt gcattaggta actcttcagc atacaaccca agcatgctca
     5161 agctttcggc cagcaactca aaagttgatt gttccagata acctatatgc tctttaaaat
     5221 attgaacaaa gcccaaactc aaatcgctga tagatttctg cgttggcatt tccagcatat
     5281 caaaggctgc tgaaacttgt tgcaacggct tgacagtgtc atctaataac gcagcatctg
     5341 ttgggtttct aaagaatgca tcaaacgctt tttcagcatg tagcaaagct gccagcactt
     5401 gctttgcaac ggcttccata acacttgcat ctagtttagt ttcattttta aagacttcta
     5461 atggcggagc gagcggcacg ccatctacca cggcaagtaa acgcgccgtt tgcgtggtaa
     5521 tttttgcctg catttgcaca tcaagttgtg cataatgctc gctgatatac tccagcaaat
     5581 taagggcagc agcaatctca attaaggaga cttcattcac ttttaaaggg tcagtatgca
     5641 gcgcacttgc cgccgcatag attgcgttca ccaaggcata agccgcctga ttattcaggc
     5701 cttgctgtac gttacttagg tgttgaaatt ttcctgcgaa cgtggctaag tcttctgtcg
     5761 atgaggggtt ttcgctgatg cgcgtccaca agtcttttaa tattggcaaa tctgcgctca
     5821 actgtgccag tgcggtgcgt tcagcgtcgg tggttaaatt accctgtcca gatgcacctg
     5881 tcacatttaa cggtagaact tgatcaagct cgaatgtttc tttaactcta gcaatggttt
     5941 ctgttgttct atcgcttatc gccacataat acagaacatc acgcagcaac tgagaaggtg
     6001 ttttagcatc gccttcagcc atattgcgta attgctgatc aagccggcgg cataattttt
     6061 tagccccaga taaagctgcg attttttctt ggctaagtgc ctccatcaac gcaattgcag
     6121 cccaccaaag ggttttctgc gcagatttat gttgtacttg ctgcactttg acaacagcct
     6181 ctcgcatgtg cgacagggct tcagaatcat gattgcgcag ccaatcgagt agatattttt
     6241 gaaaaaccgt acgttgctct gcaacaagca gtggcacagc aaattctggc aattcactcg
     6301 taggcaaatc tttaggtgca ttaaagcctg tgtcaggaaa aaataaatcc gtgatttcca
     6361 gcgcctcacc ttgggcttca accaaaggtt taatactttc aaaaagccta gtcggcacat
     6421 ctggatagcc gtttagtaag tcttgcaaat actgctctaa ggttgatatg gcgtgactca
     6481 aatgcgccat taacgcttgt gttactggaa tctcttgttt ttctagtttg ctcgttagtt
     6541 tttcaatttc tgcacaatat cgcttgcaac cttcaaggcc caccatatcc aatgcgccac
     6601 tgatctgata taaatgcgca atcgtaaagc gtagcgttgc aaactcttct ggcttttcag
     6661 tcactgcggc aaaattgtcc tgcacttttt ttagtgagcg atcaatctca tctttcaccc
     6721 aagacaaagg gcctgcatca aatacttcag ccatgctgcc cccctgtttt gttacgaaaa
     6781 ccattcgtta gtgttttcat tcgaatcttc actgagctta tcaattaatc tcgttttaat
     6841 atggatgtta ttgtgcgtta aagcttaaag ccagccacag aagtacgaag ttcttcagca
     6901 agagacgtca attgacctac cgcctcagct gtcagcttag taccatcaga cgtttgggtg
     6961 gtaatctctt gaatcaactg catgttattc gccaccacag aggcagactc agtttgcgca
     7021 gaggtcgcgg ttgaaataga ctgaatgagt cgcgccagat tcactgtcac ggactcaatc
     7081 tcagtcagcg cttgaccagc agcatcggcc acacgtgcac catccaccac gccctcggta
     7141 cttttctcca tcgccgctac ggcgccgtgg gtatctgttt gaatagtctt cacaattgcg
     7201 ctaatttgct tagtcgcttc acttgaacgt tccgccaagc gctgcacctc ttccgcaacc
     7261 acggtaaagc ctcgacccgc ttcaccagca gatgccgcct gaatggccgc attcaacgcc
     7321 aaaatattgg tttgctcggt aatgtcggaa atcaattcca caatttcact aatttcttgt
     7381 gagctttcac ccaaacgttt aatccgtttt gaggtttctt gaatttgtgt acgaatatcg
     7441 ttcatccccg caatggtatt ttgcaccgct tgcgaacctt ggtttgcggc ttccagtgag
     7501 cgttgcgcca cttcagacgc ctgcgtcgcg ttgttagaaa cctgcaaaat agaacgtgtc
     7561 atgtctgtca ccgcatctgt cgtttgcaaa atttgctctg actgcgtttc agcagcgctt
     7621 aacaacttgg ttgatgtttc ttgcgcaact tctgtcgctg aggtcacctg ctccgtcgct
     7681 cggttaatct ccagcaccaa gtcccgtaaa ttatcaatcg tgtagttaat tgagtctgca
     7741 atcgcgccag taatgttatc tttaacctgt gcttttacac ttaagtcacc atcagataaa
     7801 tcacccattt catcaagtaa ctgtagaaca gcctcttggt tctcctgacc atcttttgca
     7861 agctgttcgg taagtttcat cgcctgcgtt gcttgctttt taccaaaaaa gtacatcaac
     7921 gctaataaag ttaaacccaa cagcaacaaa cccgccagca caagctgaat aaagtttgtt
     7981 tgtgattcga gcagtttggt gttttcacta gccactgctt cacgcagcgt gttacctgtt
     8041 tggttggtta tttcaaacgc agctgtcaac tctgaaagcg gagccgaagc cgctaccgtg
     8101 cccaagggca aagaaggaat aaagcgattt tttgctacca agaaaaagtt ttcacctgct
     8161 ttaatcgaag caagcaacgc gccttttaat tcaggatttt caatattttc ttgccagtaa
     8221 gcagaacgct ccctgaataa agactcaaac cttgctgcct ttgccagcgc tttatcccgt
     8281 ttatcattat taaaattgga tgatgccaca taggcttcgt tatacgcggc aaacgcatct
     8341 acagggtata gcggtggcgg caccgtgtcg gcaaccaacg tgtcaaagtc atgcagtgcg
     8401 ttatatacag gaccgttcac acgcacctga ttaatcaccc aaaaactcag tgccgcaaaa
     8461 atgataaacg ccgcaatttg caagtaaagc tgttttaagc cagaacctac agtttcagaa
     8521 cttgaagcgc tcgctttttt gctagttagc ttactaaaaa accctccaag taactgtggc
     8581 aagcttttta caaccgctag atttaacgcc attttttacc ccaatccata aaattataat
     8641 tttttataat taaaattttg tttattaaaa tcagatgctt tagtactaaa ttacaacttt
     8701 aatctcgcta cacaagttca aaaaatgcta tatcgttggt tgaataaagt cttgatgctg
     8761 cacaagcgct tcaatattta actcttccca cgtttgctga ttttcatctt catacacttg
     8821 cttaatcaat aaatccagca aacctcgctc aataatggct ttttgcttca ccttcatcga
     8881 ctcaatgttc cgtaaaccta ctaagctagt tacaaccagc gcagtttgcg tagtgttgag
     8941 cgagttcaat aataaaattc ggctgtgtat ggtttttggc aatgctggct tgccaataaa
     9001 ttgcagcaag tcggtaatat tgtataaatt accgcggacg ttagcgacac ccaaaaacca
     9061 aggctgtgtg agcggaacgc tctgtagcgt tggcactggt aacacctcgg tcacggcggt
     9121 taaattaatt aacaatcgat actcacccac catcacgcct aaacgtgatg tggattctgc
     9181 cgcaccttgc gaagtggctt cttttagctt ttgaagaatt ccttcttgaa actcacgtaa
     9241 atttgaggtt tttgccatgt tttaagtgtg gatgaacgac aatatctaaa ctgataaaaa
     9301 aaacaaagct taacctagcg ttttaatcag cgccaataaa tcagcgctca ccacaggctt
     9361 agtcacacag gctttggcgc ccattttaag cgcccaagcc aagtccgttg cctgcgattt
     9421 acctgtacat aaaatcactg gaatattggt cgtttttggg tctttagtca gcatgcgcgt
     9481 cacctgaaag ccagttaaac ctggcatcac aacatccatc agtactaaat caggtggatt
     9541 tgttgcgact ttatccaagc aatcttcgcc actgtctgca cccgtgacgc taaagcctgc
     9601 tgtttctagc aactcagtta aaaaaaatcg atccgttgcc gagtcatcta ccactaatat
     9661 tttttcaatt gccatatcaa tctgcctctt cttgattatt tttgcctaac gacgcatacg
     9721 tttgcacagc atcaacaagc gtttcttgtg taaatggttt ggtgagatac tgatctgaac
     9781 ccaccatgcg cccgcgcgca cggtcaaata agccatcttt gctagatagc ataatgactg
     9841 gggttgtttt atagcgcgcg tttcgcttga tgagcgagca tgtttggtag ccatccaaac
     9901 gcggcatcat gatatcgaca aagataatat caggatgttc attggcaatt tttgccagtg
     9961 catcaaaccc atcttccgct agaatgactt tgcagccaga gtttttaaga aaaatctcag
    10021 cgctgcgccg aatggtattg ctgtcatcaa tgaccatgac tttaactgcc gacaacgcgt
    10081 ctaagctgag tttttttgcg ttcgattgat cttcttgact tggcttatcc agactcacaa
    10141 gcaagctcct agttatcatt gttgcgctga cgactgcgca aattcactaa aattgttaca
    10201 attaatttgg ttacattata tgtaaaatca cgcaataagc aatactatgt aatgtattac
    10261 attaatatat agtggtatct atgtaatatt ttgcattttc aaccataatg gtatagattt
    10321 acaaacttta caattgcctc acaaaaattt aacaatttgc aactttagtt tatagctcaa
    10381 gactaaagta aaaacatact taaatcttag ggattacttt catgatcgtt cgacaagaaa
    10441 tactgaatgc caaaaca
//
LOCUS       DAOQSI010000061         9976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_6094_length_9976_cov_5.149380, whole genome shotgun sequence.
ACCESSION   DAOQSI010000061 DAOQSI010000000
VERSION     DAOQSI010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 9976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9976
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6094_length_9976_cov_5.149380"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PKL42_05390"
     CDS             <1..220
                     /locus_tag="PKL42_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66543.1"
                     /translation="IYHTSCQYGFWHGQNAGRLGNDCSPIFTTTGPVLIAYLASHLRR
                     ENFIALRAFIYKASRASKKVGCKNGIKT"
     gene            413..901
                     /gene="fliL"
                     /locus_tag="PKL42_05395"
     CDS             413..901
                     /gene="fliL"
                     /locus_tag="PKL42_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HNU66544.1"
                     /translation="MAADPKPEAAEAAPKSKKKLIIIIAAVVLLAGGGGAAWFFTQSK
                     ADHKKEVKHEEVEKAPVFVTLETFTVNLQPDPDEKFLQLDITTQVATPEAAEQFKAQM
                     PAVRNRLLMLLTSKMASEISSTEGKQALSEEITAELKKPFSEKGKPLEISGVFFTSFV
                     IQ"
     gene            935..1939
                     /gene="fliM"
                     /locus_tag="PKL42_05400"
     CDS             935..1939
                     /gene="fliM"
                     /locus_tag="PKL42_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HNU66545.1"
                     /translation="MADKFLSQDEVDALLKGVDGDEDDGQSKGDESEVRPYNLATQER
                     IVRGRMPTLEIINERFARLLRIELFNFLRRAVEVSVGPVKITKYSDFIRNLVVPTNLN
                     LINIHPLRGTALMVFDPTLVFLVVDNMFGGDGRFHTRIEGRDFTQTEQRIIQRILNIV
                     FDTYSKSWEPVYPVRFEYIRSEMNTQFANIATPNEVVVEVTFNIELGPASGEVHLCIP
                     YSMIEPIRDLLASPLQGEVLGVDKRWVKLMTQQVQAAEVEIVTDLAKTNMRLGDVLNM
                     KVGDVIPLQIDEAVEANVDGIPVMLCKYGIFNGQYALRVEKLLRSNSTEYIKGEPYGD
                     "
     gene            1929..2366
                     /gene="fliN"
                     /locus_tag="PKL42_05405"
     CDS             1929..2366
                     /gene="fliN"
                     /locus_tag="PKL42_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HNU66546.1"
                     /translation="MATDTVDENDVEEIADDVWGAAMAEQSAAETSSVLQNSSADQSQ
                     VFTEFSAKNKLHETQNDIDFILDIPVQLTVELGRTKIAIKNLLQLAQGSVVELDGMAG
                     EPMDVLVNGCLIAQGEVVVVNDKFGIRLTDIITPAERIRKINR"
     gene            2376..2861
                     /gene="fliO"
                     /locus_tag="PKL42_05410"
     CDS             2376..2861
                     /gene="fliO"
                     /locus_tag="PKL42_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HNU66547.1"
                     /translation="MKVKHATLILAGLLSAPINILAATATSTAPSTSGSLFKMFLGLA
                     VVLLVMAAITWALKRIVPGAGGQQSVIKVVGGVSVGTRERVVVVEVAGRWLVVGVAAG
                     QVNAIANLDKTTDSLNDALQNTPANAEAGLQGFTQSLPPQFSTWLKKSKDKLAAHSNA
                     K"
     gene            2851..3606
                     /gene="fliP"
                     /locus_tag="PKL42_05415"
     CDS             2851..3606
                     /gene="fliP"
                     /locus_tag="PKL42_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147712.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HNU66548.1"
                     /translation="MQNNPSKIIALLCLAAGLLLPISTFAENGISVINATPAAGGGQN
                     YSLSLQTLILLTSLTFIPAVVLMMTGFTRIIIVLSLLRQALGTQSAPPNQVMIGLSLF
                     LTFFVMSPVIDKIYTDAYQPLAENRISTEQAIDIGSKPLKEFMLKQTRESDLALFVKM
                     AQIEKIDTPEQVPLKVLVPAFMTSELKTAFQIGFAIFIPFLIIDMVVASVLMSMGMMM
                     VSPAIVALPFKLMLFVLVDGWALLMGSLVQSFF"
     gene            3659..>3865
                     /gene="fliQ"
                     /locus_tag="PKL42_05420"
     CDS             3659..>3865
                     /gene="fliQ"
                     /locus_tag="PKL42_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HNU66549.1"
                     /translation="MTPESVMSLGGEAMQITLMVAAPILLTVLIIGLVVSIFQAATQI
                     NEQTLSYIPKLVGVFVALIFTGPWM"
     assembly_gap    3866..3965
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3976..4083
                     /locus_tag="PKL42_05425"
     CDS             3976..4083
                     /locus_tag="PKL42_05425"
                     /inference="COORDINATES: protein motif:HMM:NF013480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66550.1"
                     /translation="MVGVFVALIFTGPWMLTTMVDYMHRVFTSIPAMAN"
     gene            4111..4914
                     /gene="fliR"
                     /locus_tag="PKL42_05430"
     CDS             4111..4914
                     /gene="fliR"
                     /locus_tag="PKL42_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HNU66551.1"
                     /translation="MISISSELLQTWIIALLWPLTRVLGFIAVAPIFSHKAVPNQVKL
                     GFAMMLTLMVMPILPPLPQFEIFSWYGLLLLVEQLVIGLAIGFSMRVVLAAIDIAGQI
                     IGMTMGLGFASFFDPNTHGQSTALTQFLILITMLVFLSLDGHLLMVSAVADSFTTMPI
                     ALNGAGIQPMKIVNWAEAIFSTGLLLSLPAITALLMTNMALGILTRTAPQLNLFGIGF
                     PITLSMGLLIILLTLPGMLKPIENLIEQGISHMSEIATNPSAIDPILKP"
     gene            complement(5087..6001)
                     /gene="flgL"
                     /locus_tag="PKL42_05435"
     CDS             complement(5087..6001)
                     /gene="flgL"
                     /locus_tag="PKL42_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HNU66552.1"
                     /translation="MRISTNTLYQAGFAKLSDLQSNQSKLQQQIATGRRILSPSDDPI
                     ASARALEVSHEKNVNNSFADTRKVAQLKLNTLEANLTSVTNLLVATQSSLVAAGNGAL
                     SNAERKIIGTELQGSLEALIGLANTKDAAGNFIYSGFKSDTAAFTATPTGATYNGDAQ
                     QQLLQVDPQRQMSVNVTGDTLFLNGSNVFSTLKDIVTLLNTPITDATTQANFTSGLSS
                     AIGKLQGSVDNVLNVRTAIGTKLNELDALDVAGTDRDLQYSQSLSDLQDLDYTAALTD
                     LAKQQTIIEAAQKSFVTTTSLSLFDYMR"
     gene            complement(6034..7995)
                     /gene="flgK"
                     /locus_tag="PKL42_05440"
     CDS             complement(6034..7995)
                     /gene="flgK"
                     /locus_tag="PKL42_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HNU66553.1"
                     /translation="MATSNILSIGKTALNAAQVGISTTGHNIANASTAGYSRQVVVQS
                     AASSQNFGYGYVGQGTEVSSVTRVYNDLLARQAINSQSVSAGLETYAANMSSIDNMLS
                     DASAGLSPTLNDFFASVKALSANPNDPSTRQTMLSNAQSLVNRFHSTNSRLDEIREGI
                     NTQLSSSVGLVNTYAKQLSSLNDTIDKAISATGNQPNDLLDQRDQLVTELSKLIKTTV
                     VPQGQGGYNVFIGNGLPLVVGTTSYNLTTASSPTDPSRLEVAYHSNNKTSILSTESLP
                     GGSIGGLLQFRSESLDAIQNKIGQIAVSIAGTFNAQHAQGTDKNGNPGGNLFTIAAPI
                     VNSNSNNTGSAVISSQVVDASAVTSSDYRLQYDGANYKITRLADQSVQTFASLPQTVD
                     GLSFTQASGTINANDDFVIRPTLNAGNTLALAFSDVNKLAVGGPVLSSSANAGNTGSA
                     NLSALSVNSSYAASPLASPLTFTYTSGIPGTFAMAPSALPVTVTNGGSSTTYPPGAPI
                     TYTSGATISVGSLSFALSGTPANGDEFTVAPSASTGPSDNRNGLLLANLETKGNVQAI
                     GSTTKNSYNAAFAQMVSGVGNKTRELKVTSAAEAQVLEQANTAMQSESGVNLDEEAAN
                     LLRYQQAYQAAGKMMQIASQLFDVLLNIR"
     gene            complement(8077..9057)
                     /gene="flgJ"
                     /locus_tag="PKL42_05445"
     CDS             complement(8077..9057)
                     /gene="flgJ"
                     /locus_tag="PKL42_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HNU66554.1"
                     /translation="MINTSELTGKVAFDANGLNGLKNAAKENSPEAIKEVAKQFEAIF
                     MNMMLKSMREASSSEDNPFDNEQSRTFTAMLDQQLSSNLSSKGLGLADVLAQQLSKTG
                     YGKVNEMQQLKDAATDNTTKNDTSSLINPSIQNLSHLKQNTSSISQANFNANPIINNA
                     LNAARKVETNKPVELSVTAFQAKMAAHAEEASRTTGIPTNLMLGQAALESGWGKREIK
                     GADGTNSHNLFGIKATGGWDGKVVETMTTEYINGIKQKRIEKFRAYDNYADSFKDFAK
                     LMRQNPRYENVMANMHNPQAYAKAMQQAGYATDPQYASKLANVIEKVSAS"
     gene            complement(9059..>9976)
                     /locus_tag="PKL42_05450"
     CDS             complement(9059..>9976)
                     /locus_tag="PKL42_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HNU66555.1"
                     /translation="VQSILSMMQQMGVSLPPGTNLQLKNVAAVMVTSSLPAFAQPGQT
                     LDITVSSMGNAKSLRGGTLLMTPLKGADGQIYAIAQGNLIVGGVGASANGSQTQINHL
                     GVGRISDGATVERALPNAINQGDSINLELKESDFTTASSAVDAINRQFGQNTAVAEDG
                     RVIRVTPPTNRNRVSFLAALESINVTPAATTAKIILNARTGSVVMNRAVTLDSCAVSH
                     GNLTVVIDTSPVISQPAPLSNGQTVSTARSQIEISKEPGKVLKLNSGTNLSDVVKALN
                     AIGATPQDLLAILQAIKAAGALKADLEII"
BASE COUNT         2512 a         2068 c         2358 g         2938 t
ORIGIN      
        1 tatctaccac acctcatgcc aatatggatt ctggcacggc caaaatgcag ggcgcttagg
       61 taacgattgc agcccaattt ttaccacaac tggacctgtt ttaatcgcat atttggcatc
      121 acatttacgc cgtgaaaatt ttattgccct gcgagccttt atttataagg cttccagagc
      181 atcaaaaaag gttgggtgca aaaatggcat taaaacctag ttttttcttg cgctgatttt
      241 actattgttt agttcaggtg aatctctggc atcgcatccg caattgttaa tataacgcct
      301 ttttcccctt ctaatccacg catgttattg cgtgattttt ctccataatg tcattgtcga
      361 tttagcctaa ggctgtgaat taggcggtgt atttatagag aaaggcaata ccatggcagc
      421 agatccaaaa ccagaagccg cagaagcggc accaaaatca aaaaagaaat tgattattat
      481 catcgcggcg gttgtgttgc tggcaggtgg tggcggtgcg gcttggttct ttacccaaag
      541 taaagctgac cataaaaaag aagtgaagca tgaagaggtt gaaaaagcgc cggtttttgt
      601 cacgcttgag acgtttaccg tgaatttgca gccagaccct gatgagaagt tcttgcaact
      661 agacattacc acacaagtgg caacgcctga agcggccgag cagtttaaag cgcaaatgcc
      721 agcggtgcgt aatcgtttgt tgatgctgct taccagtaaa atggcttcag aaatttccag
      781 tacagaaggc aagcaggcgc tgagtgaaga aattaccgca gagcttaaaa agccgtttag
      841 tgaaaaaggc aaaccgctgg aaatttcagg cgttttcttt acttcatttg ttattcaata
      901 aatgtgcgaa atttaatatt gcgagtattg caccgtggct gataaatttt tatctcaaga
      961 tgaagtagat gccctgctca aaggcgtgga tggcgatgag gatgatggcc aatccaaagg
     1021 ggatgagagt gaggtgcgtc cttataacct agccacgcaa gagcgcattg tgcgtggccg
     1081 tatgcccacg cttgaaatta ttaacgagcg ctttgcacgg ctgttacgca ttgagttgtt
     1141 taattttttg cgtcgcgccg ttgaggtttc cgtcggccca gtcaaaatca ccaagtacag
     1201 tgattttatt cgtaatttag tggtgccaac caatttaaat ttaatcaaca ttcacccctt
     1261 acgtggcaca gcgttgatgg tgtttgatcc aacattagtg tttttagtgg tcgataatat
     1321 gtttggtggc gatggccgct tccatacccg tatcgaaggg cgagacttta cccaaaccga
     1381 gcaacgcatt attcagcgca ttttaaatat tgtgtttgat acctattcaa aatcttggga
     1441 gcccgtctac ccggtgcgat ttgaatatat tcgttcagaa atgaataccc aatttgcaaa
     1501 cattgctacg ccaaatgaag tggttgttga agtcaccttc aatattgagc ttgggcccgc
     1561 cagtggtgaa gtgcatctgt gcattcctta ttcaatgata gaacccattc gcgatttgtt
     1621 agcctctccc ttgcagggtg aagtattagg cgtagataag cgctgggtca aactgatgac
     1681 acaacaagtg caggcggctg aagttgaaat tgtgaccgat ttagccaaaa ccaacatgcg
     1741 gctgggtgat gtgctcaata tgaaagtggg cgatgtgatt cccctgcaga ttgatgaagc
     1801 ggttgaagcc aatgtagatg gcattccagt catgttatgc aagtacggaa tttttaatgg
     1861 gcaatatgcc ttgcgagtag aaaagctgtt gagatcaaac tcaacagaat atattaaagg
     1921 agagccatat ggcgactgat actgtagatg aaaatgacgt agaagaaatt gccgatgatg
     1981 tttggggcgc tgccatggcc gagcaatctg cggcagaaac ttcttcagtg ctgcaaaaca
     2041 gcagtgcaga tcaatcacaa gtgtttaccg aattttctgc aaaaaataaa ctgcatgaaa
     2101 ctcagaatga cattgatttt attttagata ttcccgtaca gctcaccgtt gaattgggcc
     2161 gcacaaagat tgccattaaa aacttattgc aacttgcaca gggttcggtg gtggagttag
     2221 acggcatggc gggcgagcct atggacgtgt tggtgaacgg ctgtctcatc gcgcagggtg
     2281 aagtcgtggt ggtgaatgat aaatttggta ttcgtctcac agacattatt acgcctgcag
     2341 agcgtattcg taaaatcaat cgataacaat ttgtgatgaa agttaaacac gcaacactga
     2401 tattggcggg tttgttgagc gcgccaatca atattttggc cgcaacggcc accagcacag
     2461 caccctcaac ctcaggcagt ttatttaaaa tgtttttagg tttggctgtt gtattactgg
     2521 tgatggcggc cattacttgg gcattaaagc gcattgtgcc aggtgctgga ggccagcaat
     2581 cggtcattaa agtggtgggt ggcgtaagtg taggtaccag agagcgtgtc gtggtggtgg
     2641 aagtggccgg ccgttggttg gtggtaggtg ttgcagcagg gcaggtcaac gcgattgcga
     2701 atttagataa aacaacagat agcctgaatg acgcgctaca aaatacccct gccaatgcag
     2761 aggctggcct gcaagggttc acgcaatcac taccgccgca attctcaacg tggctaaaaa
     2821 agtcaaaaga taaactggca gcgcattcaa atgcaaaata atccatcaaa aatcatcgca
     2881 ttattatgtt tggcagcagg tttgttgctg ccaataagca cttttgctga aaacggcatt
     2941 tctgtaatta acgcaacgcc agctgcgggc ggcgggcaaa actatagctt gagcttgcaa
     3001 acgctcattt tgctgacgtc gctcactttt attcctgccg tcgtgctgat gatgacaggg
     3061 tttacgcgca tcatcattgt gctgtcgtta ttaaggcagg cactaggtac gcaatctgca
     3121 ccgccaaatc aggtgatgat tggcttatcg ttatttttaa ctttttttgt gatgagcccg
     3181 gtgattgata aaatttacac cgatgcgtat caacctcttg cagaaaatag aatttctacg
     3241 gagcaggcca tcgacattgg ctcaaaaccg cttaaagaat ttatgctcaa acaaacgcgt
     3301 gagtctgatt tggccttgtt tgtaaaaatg gcgcaaattg aaaaaatcga cacgcccgag
     3361 caagtgccac ttaaagtgct cgtgcctgca tttatgacca gtgaactcaa aaccgcgttt
     3421 caaattggct ttgcgatttt tattccgttt ttaattattg acatggtggt tgccagcgtc
     3481 ttgatgtcaa tggggatgat gatggtgtcg ccggctattg tggcactacc ttttaagctg
     3541 atgctttttg tgttggtaga tggctgggcg ctgttgatgg gttcgcttgt gcaaagtttt
     3601 ttctaagaaa attaaactac aaagtttctt ttaaagtaaa aaggaaaaag gataaaaaat
     3661 gacgccagaa agtgtgatgt ccctcggggg cgaagccatg caaatcaccc taatggtggc
     3721 agcccccatc ttattgacgg tgcttatcat tggtttagtg gtgagtattt ttcaggcagc
     3781 cacgcaaatt aacgagcaaa ctttatcata tattcctaaa ttggtcggcg tgtttgttgc
     3841 gcttatcttc accggccctt ggatgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnntattc ctaaattggt cggcgtgttt gttgcgctta tcttcaccgg cccttggatg
     4021 ctcaccacca tggtggacta tatgcaccgc gtatttacca gcattccagc aatggctaat
     4081 taagcgtatt ttctttgaaa taacgttaat atgatttcaa ttagtagcga acttctacaa
     4141 acatggatta tcgcgctact ttggccgctt accagagtgt tgggttttat tgccgttgcg
     4201 ccaattttta gccacaaagc ggtacctaac caagtaaaac taggctttgc catgatgctc
     4261 actttgatgg tgatgccaat cttgccgcca ttgccacaat ttgagatttt ttcttggtat
     4321 ggtttgctgt tgttagtcga acaactcgtc atcggcctcg ccattggctt tagcatgcgc
     4381 gtggtgctgg cagccattga tattgccggg caaattattg gcatgaccat gggcttaggt
     4441 tttgccagtt tttttgaccc caatacgcac ggccaaagca cggcattaac gcagttctta
     4501 attttaatca ccatgctggt atttttaagt ctggatgggc atttgctcat ggttagtgct
     4561 gtggcagata gttttaccac catgccgatt gcacttaatg gcgcaggcat tcagcccatg
     4621 aaaattgtta attgggctga agcaattttt agcacagggt tgctcctaag tttgcccgct
     4681 attacggcac tcttaatgac caatatggcg ctcggcatct taaccagaac cgcgccacaa
     4741 ctgaatctgt ttggtattgg ctttccaatt acgctcagca tgggcttgtt gattattttg
     4801 ctgaccctac cgggcatgct aaaaccgata gaaaatttaa tcgagcaagg gatcagtcat
     4861 atgtcagaaa ttgccaccaa cccaagcgcc attgacccaa tacttaaacc ctaattttta
     4921 gaattgaacc tgagaaaacc aattaaaaaa gcacttaaaa aagtgctttt ttagtggttt
     4981 ttttgcatca aacctttttc gatgccctgc gagcctttat ttataaggct tgcagggcaa
     5041 tgttttttgc tcgggagttt ttaagtgcgt tattcgactt taaaatttaa cgcatatagt
     5101 caaacagtga caggctcgtc gtcgtcacaa aagatttttg tgcggcttca attatcgttt
     5161 gctgtttggc taaatcggtt aaggctgcgg tgtaatccaa gtcttgtaaa tcagaaagtg
     5221 attggctgta ttgcaagtct cggtcggtgc cagccacatc tagtgcatct aactcattta
     5281 atttggtgcc aatggcggtg cgtacgttca gaacgttatc cacagaacct tgcagtttgc
     5341 caatcgctga actcaagcca cttgtaaagt tagcttgcgt tgtagcatcg gtaatgggtg
     5401 tgttcaatag cgttacgata tctttcaagg tgctaaatac gttactgcca ttcaaaaata
     5461 aggtgtcgcc tgtcacgtta acgctcattt ggcgttgcgg atccacttgc agcagttgct
     5521 gttgtgcatc accgttgtaa gtggcgccag ttggtgtggc ggtaaatgcg gccgtgtctg
     5581 atttaaagcc ggagtaaata aaattacctg ccgcgtcttt ggtattggca aggccaatca
     5641 aggcttcaag tgagccttgt aattctgtcc caataatttt acgttcagcg tttgatagcg
     5701 cgccattgcc cgcagccact aggctagatt gcgttgccac cagtaaatta gtcacactgg
     5761 tgagattggc ttctaaggta ttaagcttta attgtgcaac tttacgcgta tcagcaaacg
     5821 aattattcac atttttttca tgagatactt ccagcgctct ggcagaggca atcgggtcat
     5881 cagaaggcga taaaatacgc cggcctgtgg cgatttgctg ttgcaattta ctttgatttg
     5941 actgtaaatc actcagtttt gcaaagccgg cttggtaaag ggtattggta ctaatgcgca
     6001 tcataaactc cgaaaaaaat cagtaaagct gcgttatcga atgttcaaca atacatcaaa
     6061 caattggctg gcaatttgca tcattttgcc agcggcttga taagcttgtt ggtatctgag
     6121 taaattagca gcctcttcat caagattaac gccagattct gattgcattg cagtgttggc
     6181 ttgttcaagt acttgggctt cagctgcgct cgttacttta agttcacggg ttttgttgcc
     6241 tacgccactg accatttgtg caaaagctgc gttgtaactg tttttagtgg tgctgcctat
     6301 agcttgcacg tttcctttgg tttcaagatt agcaagcaaa aggccatttc tattgtcact
     6361 tggcccagtt gaagcgcttg gcgctacggt aaattcatca ccatttgcag gtgtaccaga
     6421 aagagcaaaa ctgagactcc ccacagaaat ggtggcgcca ctcgtgtaag taatcggtgc
     6481 gcctggtgga tatgtagtgc tgctaccgcc attggtgaca gtaactggta gtgcagaagg
     6541 tgccatggca aacgtgcctg gtattccgct ggtgtacgta aaggttagcg gcgaggcaag
     6601 tgggctagcg gcgtaacttg aattaacgct gagtgcgctc aaatttgcac tgccagtatt
     6661 gcccgcattg gcacttgaac tcagcacagg gccgccaacc gccagtttat tgacatcact
     6721 aaaagccaat gccaaggtat tgccggcatt taaggtgggc ctaatcacaa agtcatcatt
     6781 tgcattgatt gtgccagaag cttgggtaaa gcttaaacca tcgactgttt gtggcaggct
     6841 tgcgaaggtt tgcacggatt gatcagctaa acgggtgatt ttgtaattag cgccatcata
     6901 ttgcaggcgg taatcgctgc ttgttacggc gcttgcgtct accacttggc tactgatgac
     6961 agctgagccg gtgttgttgc tattgctatt cacaataggt gcggcgattg tgaataaatt
     7021 gccaccagga ttgccatttt tgtcggtacc ctgcgcgtgt tgcgcattaa atgtaccagc
     7081 aatagatact gcaatttggc caattttatt ttgtatggcg tctaatgatt ctgaacggaa
     7141 ctgtaataag ccgccgatgc tgccacctgg caagctttca gtacttagaa tagaggtttt
     7201 attattgcta tggtatgcca cttctaatcg actggggtct gttggagacg aagcagttgt
     7261 caggttgtag ctagttgttc ccacaacaag tggtaagcca ttgccaataa aaacgttata
     7321 accaccttgg ccttgtggca caacggttgt tttaattagt ttacttaact cagtcaccaa
     7381 ttggtcacgt tggtctagta aatcgttggg ctgattgcct gtggcgctaa tcgctttatc
     7441 aatggtatcg tttaagcttg aaagctgctt tgcataagtg ttcaccaggc ctacactgga
     7501 ggaaagctga gtattgattc cctctctaat ttcatctagg cgactattgg tggaatgaaa
     7561 gcgattgacc aatgattgcg cattggaaag catggtttga cgcgtacttg ggtcgttagg
     7621 gttggcagat aacgctttta cactggcaaa aaagtcattt agtgttgggc ttaatccagc
     7681 cgaagcgtca gataacatgt tgtcaataga tgacatatta gcagcataag tctctaaccc
     7741 tgcgctgaca gattgactgt taatggcttg tcttgccaac aaatcgttgt aaacacgcgt
     7801 gacggatgat acttcagtcc cttgacctac ataaccataa ccaaaatttt gtgatgatgc
     7861 agccgactgg accacaacct ggcgggaata tccagcggta gatgcgttgg cgatattatg
     7921 ccccgtggtg ctaatgccca cttgagcggc gttaagggcc gttttcccaa tacttaatat
     7981 atttgaggta gccatgatgt tgccttttta tagaatctca taatgtgcat tacgacatga
     8041 attagagaaa ctttaacgtt attttttgtt gtggcttcat gaggcagaga ctttctcaat
     8101 cacattggct agtttgctgg catattgcgg gtctgtggca tagcccgctt gctgcattgc
     8161 ttttgcataa gcttgcgggt tatgcatatt ggccatcaca ttttcatagc gcggattttg
     8221 ccgcattagt tttgcaaaat ctttaaatga atcagcgtag ttgtcataag cacgaaattt
     8281 ttcaatacgt ttttgcttga tgccattgat atattcggta gtcatggttt cgacaacttt
     8341 gccatcccat ccgcctgtgg ccttaatgcc aaataaatta tggctattgg taccatcagc
     8401 gcctttaatt tcacgttttc cccagccact ttcaagtgca gcttggccta gcattaagtt
     8461 tgtagggatt cccgttgtgc ggcttgcttc ttcagcatgt gctgccattt tggcttgaaa
     8521 agcagtcacg ctgagttcaa caggtttgtt ggtctctacc ttacgcgcag cattcaaagc
     8581 attgttgatg atgggattag cgttaaagtt agcttgacta atacttgaag tattttgttt
     8641 taaatggctc agattttgta tgctggggtt aattaaagaa gaggtgtcat tttttgtagt
     8701 attgtcagtt gcggcatctt taagttgctg catttcattg accttgccgt agccagtttt
     8761 actgagttgt tgtgctaaca cgtcagccaa ccctaagcct ttactgctca ggttgctgct
     8821 gagttgttgg tcaagcattg ccgtaaaggt gcggctttgt tcgttatcaa aagggttgtc
     8881 ttcagatgaa gaagcctcac gcatactttt gagcatcatg ttcatgaata ttgcttcaaa
     8941 ctgctttgca acttctttaa tcgcttcagg tgaattttct ttagccgcgt tttttaagcc
     9001 attcaggccg ttcgcatcaa aagctacttt tccagtaagt tcggacgtgt tgatcatctt
     9061 aaataatttc caaatctgct tttagcgcgc ctgccgcttt aatggcctgc aaaatggcga
     9121 gcaaatcttg cggtgtggca ccaatggcat tgagtgcttt gacgacatca gataaatttg
     9181 tgccactatt aagttttagc actttgccgg gttcttttga gatttcaatt tgagaacgtg
     9241 cggttgaaac ggtttgccca tttgagagtg gcgcaggttg actaatcact ggggaagtgt
     9301 caatcactac cgttaagtta ccatgtgaaa ctgcgcagct atccagcgtt accgcgcggt
     9361 tcatcacgac tgatcctgta cgcgcgttca gtatgatttt tgctgtagtt gctgccgggg
     9421 taacgttgat ggattcaaga gctgctaaaa aagagacgcg attacgatta gtgggtggcg
     9481 tgactcgaat cacccgtcca tcttcagcca cggccgtatt ttggccaaac tggcggttaa
     9541 tggcatctac cgcagaagat gcagtagtaa agtctgattc tttgagctca aggttgatgc
     9601 tgtccccttg gttaatggcg tttggtaatg cacgctctac ggtggcgcca tctgaaatac
     9661 ggccaacgcc caagtggtta atttgcgttt ggctaccatt ggctgaggct cccacgccac
     9721 ccacaattaa attaccctgt gcaattgcat aaatttgacc gtctgcacct ttaagggggg
     9781 tcattaataa tgtgccgcca cgtaaacttt ttgcattccc catcgaagat acggtgatgt
     9841 cgagcgtttg tccgggttgc gcaaaggcgg gaagactgct ggtgaccatg acggcggcaa
     9901 catttttgag ttgtaaattg gtgcctggtg gcaaactcac gcccatttgc tgcatcatac
     9961 ttaatatgct ttgtac
//
LOCUS       DAOQSI010000062         9947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_6124_length_9947_cov_4.473110, whole genome shotgun sequence.
ACCESSION   DAOQSI010000062 DAOQSI010000000
VERSION     DAOQSI010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 9947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9947
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6124_length_9947_cov_4.473110"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="PKL42_05455"
     CDS             <1..830
                     /locus_tag="PKL42_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated putative
                     ABC transporter substrate-binding protein"
                     /protein_id="HNU66556.1"
                     /translation="NPDEGRMGEVRRVADDSEFKVCADPENMPYSNNKQEGFEDKIAE
                     VLAKDLGKKLAFTYAYSRQGFYRNTLGANRCDVVMGTNSDSDLMRTSKPYYRAGHVYV
                     WRKESNFNITDWSSPDLRKGFIGVVDHSPATIPMDDNGLIANARPYRIQRDLNLPSSY
                     MIDDLAKGDIDIAIAWGPIGGYFAKHSKIPMVVVPTPEYETQNAKGKNYWNISIGVRK
                     KDKERMAMIQGALDRNQDKINKILDDYGIPHVPVVEGDSLLKAYQKYGAGDKAPVNK"
     gene            1013..1516
                     /locus_tag="PKL42_05460"
     CDS             1013..1516
                     /locus_tag="PKL42_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNU66557.1"
                     /translation="MLSNKTLKSALFASLLAFAGLVSMNASAADISLVATQDGSPLNI
                     KKELFDTPQAKEFLATGKNPYVGNEEGIKGGKKKYNLYSCVACHGGHGEGAVAPGLIG
                     PGFKYAKNATNKGMFETIWHGTNGGMGAKGLGLMLPDDPTEGLKPDDVLKVIAFIRSN
                     SKITGNE"
     gene            1717..2271
                     /locus_tag="PKL42_05465"
     CDS             1717..2271
                     /locus_tag="PKL42_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNU66558.1"
                     /translation="MKQLKISVLLFVFIGLAACGKSESEATSGTPEAAATASSAAPAG
                     NAIAFVDTQEGKPLVIDAALFDTPAAKEFLATGKNPYVGNEEAIAKGKKVFQLYSCVA
                     CHGGNAEGAVGPGLIGPNFKYAKNATNKSMFETVWHGTNGGMGAKGIGLMDPSDPKNG
                     VTPDELLKIIAWIRSVSTGITGNE"
     gene            2361..3698
                     /locus_tag="PKL42_05470"
     CDS             2361..3698
                     /locus_tag="PKL42_05470"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNU66559.1"
                     /translation="MRDNQQQDLHHVVIVGGGAGGLELATKLGDSLGRKGKATITLID
                     HSKTHVWKPLLHEIAAGSMNPEKHELVYLAQAHWHHFKFRLGKMDALNRAKQEISIAP
                     NYDEDGNEVIPRRTFKYDTLVIAVGSTTNDFGIKGAREHSIALDTQEQAERFHRRLHN
                     ALLRAQTQTTPVQPGQLEVVIVGAGATGVELSAELHNTTRELAAYGLDKIDADRDVKI
                     SLIEASERVLPALPPKLSHSVELELKKLNVHLYTGERVTEVTSEGIHTHSGKFIPSSL
                     VVWAAGIKAPDFLHEIDGLETNRINQLVVNRTLQTTLDANIFAFGDCAACPVVSSKAD
                     ENVPPRAQAAHQQASMLYKTIKNRVAGKSHLPEYTYRDYGSLVNLGRYTTVGNLMGSI
                     SGGSLFIEGLIARLMYQSLYQMHLMALHGFWSTALQALARTISRRTESQVKLH"
     gene            complement(3834..5009)
                     /gene="lplT"
                     /locus_tag="PKL42_05475"
     CDS             complement(3834..5009)
                     /gene="lplT"
                     /locus_tag="PKL42_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HNU66560.1"
                     /translation="MAKGFYTFLIAQFLSAIADNALLFACIALLSTLNAPEWHVPFLK
                     ETFVVSYIILAPFVGSFADALPKGRVMFISNGIKFFGCVLALLGVPPLFAYAIVGIGA
                     AAYSPAKYGILTEMLPSNQLIKANAWVEGTTVSAIILGPVLGSTIAADNPYFGIAIIA
                     VVYLLAAFFNHFIPKVPIDHKVAQRSLTFLIRDFWHAFTTLWKDTQGQVALAVTTLFW
                     GAGATLQFVILQWAKEVLGMDQQAATVLIAILAIGIAVGSVGASMFVKLEEAVKVLPS
                     GILMGFLVVSMIFVDTREVAAVVLFCIGVLAGYFLVPLNSLLQHRGHILLGAGHSIAV
                     QNFNENIGILLLLGIYTFMVHQNLPINFIIIIFGIFVSVSMAVIYQLYLKHTRANAA"
     gene            complement(5012..6409)
                     /locus_tag="PKL42_05480"
     CDS             complement(5012..6409)
                     /locus_tag="PKL42_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNU66561.1"
                     /translation="MAKKTSSNNKLFVLDTNVLIHDPSSLFRFEEHDIFLPMVTLEEL
                     DNNKKGLTEVARNARQASRNLEEIVGTDLVNLELGCPLGVSGNKHLTGRLFLQTMQVS
                     AELPGLAGFKADNQILAVVLDLKKKQPDRQVILVSKDINIRIKARAMGVPAEDYFNDK
                     VLEDTDLLYSGVKELPADFWEKHSKAMESWQDAGRMFYRINGPLCKTFLLNEFVYYEF
                     DKPFHAIVRSIENNTAVLEIVKDHQLPKHNVWGINARNREQNFALNLLLDPDIDFVSL
                     LGQAGTGKTLLTLAAALTQVLDSKRYSEIIMTRVTVSVGEDIGFLPGTEEEKMGPWMG
                     ALDDNLDVLNKSDSDAGEWGRAATQDLIRSRIKVKSLNFMRGRTFLNKFLIIDEAQNL
                     TSKQMKTLITRAGPGTKVVCLGNIAQIDTPYLTEGSSGLTYVVDRFKGWSHNGHITLM
                     RGERSRLADFAAEAL"
     gene            complement(6421..6879)
                     /locus_tag="PKL42_05485"
     CDS             complement(6421..6879)
                     /locus_tag="PKL42_05485"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNU66562.1"
                     /translation="MLNTSVSDFELPATSGKTFKLSNFIGKNLILYFYPKDSTPGCTT
                     QGIQFRDAYDQFQAHHTEIFGISRDSLKSHENFKAKFSFPFELLADTEELACQLFGVI
                     KMKNMYGKQVRGIERSTFIIDKNGKLVKEWRGVKVDRHVNEVLNFIQTLN"
     gene            complement(6979..8454)
                     /locus_tag="PKL42_05490"
     CDS             complement(6979..8454)
                     /locus_tag="PKL42_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNU66563.1"
                     /translation="MSKKNKSLKDLLLIHELAFILLIFFAGAAGTYGIHLWEKSSQEA
                     SRISALINEVQQTRGDLYRQMKELFDAYFLEDITAKDEYNEFTKSVEKHFTLLDTLAV
                     GADEKAAIDDLYANYKQFVIETPILFDAFQNANTEDLKRALNTDIETGLFKRFEDILS
                     RTEQLLNQKQMQLDHQLTRTKRITSQLLIIPLLLAVLLLIFSRIFLKRAIVQPISGVL
                     KATSEISSGNLAHKAPEEGVAELAEVSKAINEMAGQLASSQEALVRTEKQAAQGLLVP
                     MLAHNIRNPLASIRATAQVMDDPTHDTETRESLLGIINTVDRLERWTGALLAYLLPLK
                     PQPTHTSIQTIVEGALGPLQQKIKEKSIQLNLPAWDNSTAIYTDQHLLEQVIYNLLLN
                     AADASKASATLDISYTNSAQTIYLRIADRGSGMPFTPDPSAISAPTTKRFGTGLGIPF
                     AFKVCDALGGELNFEPRVDGGTIVTITLPKQMTSTQTNRLS"
     gene            complement(8500..9765)
                     /locus_tag="PKL42_05495"
     CDS             complement(8500..9765)
                     /locus_tag="PKL42_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNU66564.1"
                     /translation="MSNITTLSNLANNPNSMEDYDPNAMSVSQARQFIQQFLSPISAT
                     ETLPIRESLGRVLSANVISPNNVPNYNNSAMDGFAFRFSEGVKIIKVIGTAFAGRPFT
                     DELKAGECVKIMTGAMIPAGADTVVMQEHVVVKSENISLLSNIKPGANVRLAGEDLRK
                     GQKVLGRGHLMRPADLGLVASLGIGEVSAYKKIKVAFFSTGDELKSLGNPLAEGQIYD
                     SNRYTLFGMLSRLEVDVHDLGAVPDDPNLLETTLLKAASENDVVITSGGVSVGEADHM
                     KTLLAQHGQVLFWKINMKPGRPLAYGKIGNAHYFGLPGNPVATMVTFYQFVREAIITL
                     MGATYQPLPLFKVECTEPIKKATGRTEFQRGLLFDVDGIWKVKPTGAQGSAILSSMSQ
                     ANCFIVLDESTGNLEARATVQVQLLDGLV"
BASE COUNT         2858 a         1998 c         2270 g         2821 t
ORIGIN      
        1 taaacccaga cgagggacgt atgggtgaag tgcgacgtgt cgcggatgac tccgaattta
       61 aagtgtgcgc agaccctgaa aacatgcctt actcaaataa caagcaagaa ggttttgaag
      121 ataaaattgc tgaagtactc gcaaaagact taggaaaaaa actcgcattt acttacgctt
      181 atagtcgtca agggttttat agaaatacat taggcgcaaa tagatgtgat gtggtgatgg
      241 gcactaactc tgatagcgat ttaatgcgta catcaaaacc atattatcga gctggccatg
      301 tatacgtgtg gcgaaaagaa agcaacttta atattactga ctggagttcg ccagatttac
      361 gcaaaggctt tataggcgtt gttgaccaca gccctgcaac tataccaatg gatgataatg
      421 gattgattgc aaatgctcgt ccttatcgaa ttcaacgaga tttaaattta ccatcaagtt
      481 atatgattga tgatttggca aaaggggata tagatattgc aattgcctgg ggaccaatcg
      541 gtggttattt tgcaaaacat tcaaagattc caatggtagt agttccaaca ccagaatatg
      601 aaacacaaaa tgccaaaggt aaaaattatt ggaatatctc tattggcgtt cgcaaaaaag
      661 ataaagaacg tatggcgatg attcaaggtg cactagatag aaatcaagat aaaatcaata
      721 aaattttaga tgactacggt attccgcatg tgccagtggt ggaaggtgat agcttgctta
      781 aggcgtatca aaaatatggc gctggcgata aagcacctgt aaataagtaa tttttacatt
      841 taaacgttct ttgatgttta agccagcctg aaatacgtgt aaaattgtaa gtattatttt
      901 ggttaaagcg tcaaccgatt aaataataga atcgtttaag ggtcgtacgg cgaagaatgc
      961 gggagtgttt accagtcgta actcaattta gtgatataga ttaggagata gcatgttaag
     1021 caataaaact ttaaagtctg cattgttcgc tagtttgtta gcttttgcag gtttggtttc
     1081 aatgaatgca agcgcagctg atatttcgct tgttgcaact caggatggct caccgttaaa
     1141 cattaaaaaa gagttgtttg acacacctca agcgaaagag tttttagcaa caggtaaaaa
     1201 cccgtatgtt ggtaatgaag aaggtatcaa gggcggcaaa aagaaatata acctttactc
     1261 atgcgttgct tgtcatggtg gtcatggtga gggtgcagtt gcacctgggt taattggccc
     1321 tggctttaaa tatgcaaaaa acgcaacaaa caagggtatg tttgaaacta tctggcatgg
     1381 cacaaacggt ggtatgggtg ccaaaggctt gggtttgatg ttacctgatg accctacaga
     1441 aggtttgaaa ccagatgacg ttttaaaagt aattgctttt attcgtagca attcaaaaat
     1501 cactggtaat gagtaataca aaagcctgat tattgggcga ttaggatatt ttaaaaggct
     1561 acttcggtag ccttttttat tgagtaattt ttacttatgg ataaacctag gttgctgttg
     1621 ctgtgatttt ctggaacagt aacaaattgt tacggatatt gtataatgcg ttgataatta
     1681 tttctaaagt gttgtttagt ttataggagc gtaagaatga agcagttgaa aattagtgtg
     1741 ttgctattcg tattcattgg tctagcagcc tgtggtaaat cagagtcaga ggcaacttct
     1801 ggcacgcctg aagcggcagc aacagcttca tctgcagcgc cagctggcaa cgctatcgcg
     1861 tttgtagata cgcaagaggg taaaccgtta gtgattgatg cagcactatt tgatacgcct
     1921 gcagcaaaag aatttttagc gacaggcaaa aacccatatg taggcaatga agaagcaatc
     1981 gcaaaaggta aaaaagtatt tcagctttat tcatgtgttg cttgtcatgg tggcaatgct
     2041 gaaggcgcag tagggccggg tttgattgga ccaaacttta aatatgccaa aaatgcgact
     2101 aacaagagta tgttcgaaac cgtgtggcat ggtaccaatg gtggcatggg ggcaaaaggc
     2161 attggcttga tggacccaag tgatccaaaa aatggggtta ctccagatga attgttaaaa
     2221 attattgctt ggatacgtag tgttagtacg ggtattacag gaaacgagta atttttaaaa
     2281 tgtttatatt gcgcgcttac tgtgatggta agcgcgtttt tgttttataa ggctttaaaa
     2341 gaagctaggc ggataattca gtgagagata atcaacagca agatttgcat catgtcgtga
     2401 ttgtaggcgg tggcgctggc ggccttgaac tggccactaa gctcggtgat tcattaggcc
     2461 gcaaaggtaa ggcaacgatt acgcttattg accattctaa aacccatgtg tggaagccat
     2521 tgttgcatga aattgcggca gggagtatga atcctgaaaa gcatgagttg gtctaccttg
     2581 cgcaagcaca ttggcatcat ttcaagtttc gtttaggcaa aatggatgcg cttaaccgag
     2641 caaagcagga aatttcgatt gcgccaaact acgatgaaga tgggaatgag gtgattccac
     2701 ggcgcacttt taaatatgat acgttagtga ttgcggtggg cagtaccacc aatgattttg
     2761 gcatcaaggg cgcgcgtgag cattcaatcg cattggacac gcaagagcaa gccgagcgct
     2821 ttcaccggcg tctacacaat gccttgttgc gtgcgcaaac gcaaacaacg cctgtgcaac
     2881 ctggccagtt agaggtggtg attgttggtg ctggcgctac aggtgtggaa ctctctgctg
     2941 aattgcacaa tacaacacgt gagctagccg catacgggct tgataagatt gatgctgatc
     3001 gggatgtgaa aatttcactg attgaagcca gtgagcgcgt gttgcctgcg cttccgccca
     3061 agctctcgca ttcggttgag ctggaactca aaaaactaaa tgtacattta tacacaggtg
     3121 agcgtgtgac ggaagtcacc agtgaaggca ttcataccca tagcggaaaa tttattccat
     3181 catcattggt ggtgtgggcc gcaggcatca aggcaccaga ctttttacat gagattgatg
     3241 gcttagaaac caatcgtatc aatcagcttg tggtgaatag aacactgcag accaccttgg
     3301 atgcaaatat ctttgcattt ggtgattgtg cggcttgccc tgtagtgagc agtaaagccg
     3361 atgagaacgt gccgccgcgc gcgcaggccg cgcatcagca agcttcgatg ctctataaaa
     3421 ccattaaaaa tcgcgtggca ggtaaatcgc atttacctga atatacttac cgagattacg
     3481 gctctttggt gaacctcggc cgctatacaa cggtaggtaa tttaatgggt tctatttcag
     3541 ggggcagttt atttattgag gggctgattg cgcgattgat gtatcaatcg ctctaccaaa
     3601 tgcacttgat ggcattgcat ggcttttgga gtactgcctt acaagcgctg gcacgcacca
     3661 tttcaagacg tactgaatcg caagttaaac tgcactaatt tgatacataa tgacgttgaa
     3721 taaaagtaac aaaattgact tattttctcc gagcaaaaaa caatgccctg caagccttgt
     3781 aaataaaggc ttgcagggca tgtcaaaagg tttgttgcta aaagttgctt aaattacgct
     3841 gcgttagcac gcgtatgttt gaggtaaagc tggtaaataa cggccatgct gacgctgacg
     3901 aaaataccga aaataatgat gatgaagttg atgggcaggt tttgatgcac catgaacgtg
     3961 taaatgccca acaacaataa aataccaata ttttcattaa aattttgcac tgcgatggag
     4021 tggcctgcac ctaataaaat atggcctctg tgctgcagta atgaatttaa tggcactaaa
     4081 aagtagcctg caagcacgcc gatacagaac agtacaaccg cagcgacttc acgcgtatca
     4141 acaaaaatca tgcttaccac taaaaagccc atcaaaatac ctgaaggtag cacttttacc
     4201 gcttcttcta atttaacgaa cattgaagcg cccacagagc ctacagcgat gccaatcgcc
     4261 aagatggcaa tcagcacggt agcggcttgc tggtccatgc caagcacttc ttttgcccat
     4321 tgtaaaatca caaactgcaa tgttgcacct gcgccccaaa ataacgtggt gacggctaaa
     4381 gctacctgac cttgcgtatc tttccaaagc gttgtgaatg cgtgccaaaa atctcgtatt
     4441 agaaaggtca aactacgttg cgcgactttg tggtctatag gtactttggg aataaagtgg
     4501 ttgaaaaaag ccgctaataa ataaacaact gcaataatgg caatgccaaa atagggatta
     4561 tctgcagcaa ttgttgaacc caataccggc cctaaaatga tggcgctaac ggtagttcct
     4621 tcaacccagg catttgcttt gatcagttgg ttgctaggta gcatttcagt caatatgcca
     4681 tatttggccg gtgaatatgc ggcagcacca ataccgacaa tggcataggc aaacaacggc
     4741 ggtacgccaa gtaacgccag cacgcagcca aaaaatttaa tgccattgct gataaacatc
     4801 actctgcctt ttggcagggc atcagcaaaa gaacctacaa acggcgcgag gataatatag
     4861 gaaacaacaa aggtttcttt taaaaatggc acatgccact caggcgcatt aagggttgag
     4921 agtaaggcaa tacaggcaaa tagcagtgcg ttatctgcaa tagcggataa aaattgtgca
     4981 attaaaaagg tgtaaaagcc cttagccatc gctatagggc ttctgcagca aagtcagcca
     5041 agcgtgagcg ttcgccacgc attaaagtga tgtggccatt atgcgaccag cctttaaagc
     5101 ggtctaccac gtaagtaagg cctgaactgc cttcagttaa gtaaggcgta tcaatttgcg
     5161 caatattgcc taaacaaacc actttggtgc cagggccggc gcgcgtgata agcgttttca
     5221 tttgtttgga ggtaagattt tgcgcttcat caataattaa aaatttattt aaaaaagtac
     5281 ggccacgcat aaaatttagc gacttcactt taatgcggct acgaattaaa tcttgagttg
     5341 ccgcacgtcc ccattcgcct gcgtcagaat cacttttatt taatacatct aagttgtcat
     5401 ctaatgcgcc catccacggc cccatttttt cttcttcagt gcctggtaaa aagccaatat
     5461 cttcacccac ggataccgtg acacgggtca tgatgatttc tgaataacgt tttgagtcta
     5521 aaacctgcgt aagtgcggcg gcgagtgtga gtaacgtttt gccagtaccc gcttggccca
     5581 ataaactcac aaaatcgatg tcaggatcca gcaataaatt taatgcaaaa ttttgttcac
     5641 gattacgtgc gttgatgccc caaacattgt gttttggcaa ttgatggtct tttacaattt
     5701 caagcacggc agtgttgttt tcaatgctgc gtacaatggc atgaaatggc ttatcaaatt
     5761 cgtaatagac aaattcatta agcagaaatg ttttgcatag cgggccattg atgcggtaaa
     5821 acatgcggcc agcatcttgc caggactcca tcgctttact gtgtttttcc caaaagtcgg
     5881 caggtaactc tttgacgccg ctatacagaa gatccgtatc ttccagcacc ttatcattga
     5941 aatagtcttc agccggtacg cccatggcgc gcgctttaat gcgaatgttg atgtctttac
     6001 tgactaaaat aacttggcgg tcaggctgct ttttcttgag atcgagcaca accgcaagaa
     6061 tttggttgtc tgctttaaag cctgctagtc ctggaagttc agcagagact tgcatggttt
     6121 gtaaaaacaa acgaccagtg aggtgcttat tgccgctgac accgagagga cagccgagct
     6181 caagattaac taaatcggtt ccgacaatct cttccaaatt acgactagcc tggcgagcgt
     6241 ttcgcgcaac ttcggtcaac cccttcttgt tgttatcgag ttcttctagc gtcaccattg
     6301 gcaaaaatat gtcatgctct tcaaagcgaa atagggaaga ggggtcatga attagaacat
     6361 tggtatccag cacaaagagt ttgttgttgc ttgatgtttt tttagccatg atgatgtttt
     6421 ctagttgagc gtttgaataa agttgagcac ttcattgaca tgcctatcca ctttgacgcc
     6481 gcgccactct tttaccaact tgccattttt atctatgata aaggtgctac gctcaatgcc
     6541 tctcacttgt ttgccataca tatttttcat tttgatcacg ccaaataatt ggcaagcaag
     6601 ctcttccgta tctgcaagaa gctcaaatgg aaaactgaac ttggctttga agttttcatg
     6661 agattttaga ctgtcacgcg atatgccaaa aatttcagtg tgatgtgctt gaaactggtc
     6721 gtaagcgtct ctaaattgaa tgccttgcgt tgtacagccg ggggttgagt ctttagggta
     6781 aaaataaaga attaaatttt taccaataaa attagataat ttgaaagttt tgccactcgt
     6841 ggccgggagt tcgaagtcag aaacagatgt attgagcatg gtgttatttt tttaaaagag
     6901 gttcttacgt cattgttgat aaaaagtcat gttaaggcaa tagctgaata gataaattta
     6961 tgaaatgaat acgctaattt agctcagcct gttcgtttgg gtagaagtca tttgctttgg
     7021 tagtgtaatc gtgacgatgg ttccgccatc aacgcgaggt tcaaaattta attctccacc
     7081 caaggcatca catactttaa aagcaaatgg aattcctaga ccagtaccaa aacgtttggt
     7141 ggttggtgcg ctaatcgcac taggatctgg cgtaaaaggc attccgctac cacggtcagc
     7201 aatacgcaaa taaattgttt gagcagaatt agtataggag atgtcaagcg tagcggaggc
     7261 tttggaggca tctgcagcat ttaacagcag gttataaatg acttgttcta ataaatgctg
     7321 atcagtataa atggcggtgc tgttatccca tgcgggtaaa tttaactgaa tcgatttctc
     7381 tttaattttt tgttgcagcg ggccgagcgc gccttcaaca atagtttgta tgcttgtatg
     7441 cgtgggttgc ggctttagtg gcagcaagta agccagtagt gcgccagtcc agcgctccag
     7501 cctatctacg gtattaataa ttcctaataa tgattctctg gtttctgtgt catgcgtcgg
     7561 gtcatccatc acctgcgccg tggcgcgtat acttgctaag gggttgcgaa tgttgtgcgc
     7621 gagcatcggc actaataagc cttgagctgc ttgtttctcg gtacgcacta atgcttcttg
     7681 gctagaagct aattgaccag ccatttcatt gatggctttt gagacttcag cgagttccgc
     7741 aacgccttct tcaggtgctt tatgcgcgag atttcctgaa ctgatttctg acgtagcttt
     7801 tagtacgcca gaaattggct gaacaattgc acgtttaaga aatattctag aaaaaatgag
     7861 caacagcacg gcaagaagta atgggatgat cagtaactgg gaagtgatgc gcttagttct
     7921 tgttaattga tggtcaagtt gcatctgttt ttgattgagc aattgttctg tgcgagaaag
     7981 aatatcttca aagcgtttaa ataaacctgt ttcaatgtcg gtgtttagcg cgcgctttag
     8041 gtcttctgtg ttggcattct gaaacgcatc aaagagaata ggtgtttcaa tgacaaattg
     8101 cttgtagttg gcatagaggt catctattgc agccttttca tcggcgccga cagccaaagt
     8161 gtcaagtaaa gtgaagtgtt tttctacgga cttcgtaaat tcattgtatt catcttttgc
     8221 ggtaatatct tctaaaaaat aggcgtcaaa gagttctttc atttgacggt aaaggtcacc
     8281 gcgcgtttgt tgaacttcat tgatcaatgc gctaattcta ctagcttcct ggctcgactt
     8341 ttcccatagg tggatgccgt aggtgcctgc ggcgccagca aaaaaaatga gaagaataaa
     8401 ggcaagttcg tggatgagta gcaggtcttt aagagattta ttttttttac tcatattcac
     8461 ggaggtctta tttaattaag tggttaaaca caatgaacct cacactaaac cgtccagtaa
     8521 ctgtacttga acggtggccc tagcttccag attgcccgtt gactcatcta agacaataaa
     8581 acagtttgcc tgactcatgc tgctcaaaat tgcagagcct tgtgcgccag tgggttttac
     8641 tttccaaatg ccatcgacat caaataataa gccccgctga aactcagttc gcccggttgc
     8701 tttttttatt ggttcagtac attcaacctt gaataacggt agcggctgat atgtggcgcc
     8761 catcaaggta ataatggctt cacgcacaaa ctggtaaaat gtgaccatag tagcgacggg
     8821 gttgccaggc aagccaaaat aatgcgcatt gcctattttc ccatatgcta acgggcggcc
     8881 aggtttcata ttgatcttcc aaaataacac ttgcccatgc tgtgctagca gggttttcat
     8941 gtggtctgcc tcaccaacag aaacgccacc gctggtgatg acgacatcat tttcacttgc
     9001 cgctttaagt agcgttgttt ccaatagatt ggggtcatct ggaacggcgc caagatcatg
     9061 aacatccact tctagcctgc ttaacatgcc aaataaagta tagcggttgc tgtcgtaaat
     9121 ttgaccttct gctaaaggat ttcctaagct tttcaactcg tcccctgtgc taaaaaaagc
     9181 cactttaatt tttttgtaag cgcttacttc gcctatgcct aaagaagcta caagacccaa
     9241 atcggccgga cgcattaaat ggccacgccc taacactttt tggcctttgc gtaaatcttc
     9301 cccagccaag cgcacatttg cacccggctt gatattgctt aacaatgaga tgttttcact
     9361 tttaaccact acgtgttctt gcatcaccac cgtatcagca ccggctggta tcattgcgcc
     9421 cgtcatgatt ttgacgcatt cacctgcttt gagctcgtca gtaaaaggtc taccggcaaa
     9481 agcagtgcct ataactttga tgatttttac accttcagaa aatctaaaag caaagccatc
     9541 catggcgcta ttgttgtaat taggcacatt gtttggagag atgacgttgg cagacaacac
     9601 gcgccctaaa ctctcacgaa tcggcaacgt ctccgtagca gaaatcgggc tcaaaaattg
     9661 ttgaataaat tgccgcgctt ggctaacaga catcgcgtta gggtcgtaat cttccatgct
     9721 gtttgggttg tttgctagat ttgaaagtgt ggttatattg gacattaatg ttaaaacttc
     9781 cttattattt attcccgtgt gggcgggaat ctagtcaagc ttcaaattga gcgaatgctt
     9841 gtttattgta ccaatattaa gcgaggtcta gcctagattc acgtctgcgg gtaatggcga
     9901 ttttttagtt ctagggcttc caaatcttcc agcgtgttta agttaat
//
LOCUS       DAOQSI010000063         9770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_6299_length_9770_cov_5.862790, whole genome shotgun sequence.
ACCESSION   DAOQSI010000063 DAOQSI010000000
VERSION     DAOQSI010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 9770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9770
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6299_length_9770_cov_5.862790"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /gene="pdxA"
                     /locus_tag="PKL42_05500"
     CDS             complement(<1..813)
                     /gene="pdxA"
                     /locus_tag="PKL42_05500"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNU66565.1"
                     /translation="MTAQRPRIAITAGEPAGIGLDLCVKLAQIPLAANIIVIADQYAL
                     KQRAQALNLPLHICADHICANEVPHLGNGGLQVLHHALAEPVVAGQLNPNNSTYVLNT
                     LKSAAQGCLNGTFDAMVTAPVHKGVINDAGIAFTGHTEYLAELTNTKQVVMMLVGGGM
                     RVALATTHLALKDVPAAITLASLETTIRILHADLVNKFGIPQPKIYVAGLNPHAGEGG
                     YLGMEEIETINPVLNKLRAQGFDLVGALPADTMFSPNNLKNADAFLCMYHDQG"
     gene            complement(817..2088)
                     /locus_tag="PKL42_05505"
     CDS             complement(817..2088)
                     /locus_tag="PKL42_05505"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNU66566.1"
                     /translation="MYVCLAASFNIVAAEVQKLDRIVAVVDQTVITEQELEGRIATVT
                     AQLKKQGAELPDEALLRKQILERLISDTLQIQYAAQTGLKVEDSQLDKTIERIADQNN
                     LTLTEFSEALTRDGISMRKFRNDIRNEITLARLREREVEGRVNVTESEVDNYLTNQSN
                     SGESLDEFEISHILIRTPEEGATEEIQKAKAKVEEAIKALSAGTSFAKVSASYSDAPN
                     ALEGGNLGWKKGSQMPNLFLETLKNLQIGEVSEPIRSPNGYHILKLTNKRGGNSPLVV
                     QQTRARHILIKLSEIMSEAEGKTKMENIKERLDNGEKFEILARQYSEDSTASNGGDLG
                     WVNPGDTVPQFEKAMNELRENEISAPVRSQFGWHVIQVLERRGQDMTKEAARLKARQE
                     IRARKGEEAYQDWIRELRDRAYVELRLEDKF"
     gene            complement(2130..4535)
                     /locus_tag="PKL42_05510"
     CDS             complement(2130..4535)
                     /locus_tag="PKL42_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HNU66567.1"
                     /translation="MMLLGLSQIALAAEEVSVDETHSNEPLVEASSDSATEIAAGSVV
                     VDGDKIELYLDRKMRAIGDASISQNGKTILGDTIDYDVQNEQLDVKGNAQINVGNAKL
                     TGPALSMQLSDDVGEMKDASITLFKPARENKDQLITISSEAFSKSLGNLQSQQIGGVS
                     TYTLDGSKTIADRNHTDGVTKIESGIAQDIPETRVSSARGDAQTILFEGQDTKRLKGA
                     RYTTCAPGVDDWYIKAKDIELNDFTESGVAKNAYIEFKGVPILYTPWVSFSYNNQRKS
                     GLLAPTYGTTSKSGFELTVPFYWNISPNMDATFAARALSKRGVQLQGEFRYLEPSFSG
                     IDNIEFLPSDSLSGKNRYYANLKHQHNLGRGWSAGYSLEKVSDDQYFSDMSTRIITTS
                     RVNLAQQFNVDYADETWRFNALAQKYQTLDQVSYPYERLPQMTLTGNRYFGDFNTNLY
                     TQLVAFDTNNNAPTLVTGTRFTVYPSISLPMNRSYGYLTPKIGVHHTNYSLNNDPNNQ
                     NSISRTLPIFSVDSGLFFDRDFKIARRGYSQTIEPRLFYTYIPESKQSLIPIFDTSES
                     DLNFSSLFSENQFSGNDRVNNANQLSFSVTSRLIESASGTQRLSASIGQRYYFADQKV
                     ALPGAELRKNNSSDIIAGLTTNLKTTLNLDAFWQYNTDDSQSVRTTITSRYNPEPGKA
                     LNLSYSYRRDSIDQVDVSAQWPIKPGWYGVARMNYSLREKQLIESIAGLEYNAGCWQA
                     RTLIQRVETATADANYALFFQLELGGLASIGNNPLSVIKRSVPGYVSSGLIPDSNQQS
                     YYE"
     gene            4747..5763
                     /locus_tag="PKL42_05515"
     CDS             4747..5763
                     /locus_tag="PKL42_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNU66568.1"
                     /translation="MDTRQQELNDWLTLTLPDADFTLTTASADASFRRYFRVHTHNGT
                     LIAMDAPPPQENCEPFVRIAKLFGDAGLNVPKVIAEDLERGFLLLNDLGDDTYLSKIN
                     ADNAPKLYADANNALIKLQLASQPNVLPSYDEALLTREMQLFPDWYIAKHLNVTLDEK
                     QQAVLTNTFNVLNKNILAQKQVYVHRDYHSRNLMMTQDNNPGVLDFQDAVYGAITYDL
                     VSLLKDAYISWDEELIIDWLVRYWQNARKAGLAVPEDFSEFYRDFEYMGAQRHIKVLG
                     IFARLYHRDGKDGYLKDMPLVMEYLRKVCERYVELRPMLRLLDSLSGQDQHPDANYKS
                     LLKL"
     gene            5760..6530
                     /locus_tag="PKL42_05520"
     CDS             5760..6530
                     /locus_tag="PKL42_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNU66569.1"
                     /translation="MKAMILAAGRGERMRPLTDHTPKPLLKVGGKPLIVWHIENLAKA
                     GFTEIIINHAHLGEQIEDALGDGAKWGVGIQYSPEKVALETAGGIANALPLLGDAPFL
                     VVNGDVFTGLDFSQLFENTLRPFDEFMAQGEWDKDLRLNTTVRPEPVEGRAQSNYNLA
                     HLVLVDNPPQHPKGDFAIENGMLKNVGETMLTFSGVGVYHPDLFASITRGEPAKLAPL
                     LRQAIQENKATAEYFKGVWHDIGTPERLKALDDALLRL"
     gene            6883..8205
                     /gene="pepP"
                     /locus_tag="PKL42_05525"
     CDS             6883..8205
                     /gene="pepP"
                     /locus_tag="PKL42_05525"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HNU66570.1"
                     /translation="MQTHPTEALAEFKSRRQQLIAQMVEGVAIIPTAAEAIRNRDSHY
                     PYRFDSYFYYLTGFKEPEALLVLVAGNENKSILFCRDKDMEREIWDGFRYGAEAAKAE
                     FGFDEAYSFNQLDEMLPKLLGNQTKLFYSLGADASWDARVTGWLNQVKSQARTGISAP
                     DEVFDVRKLVDEMRLYKSAYEIDVMRRSANIAAAAHNRAMQKTRAGKMEYEIEAEFLH
                     EFYRKGAQAPAYTSIIAGGANACTLHYNANNCVLKDGDLLLIDAGCELDGYASDITRT
                     FPVNGKFSAAQKDVYELVLAAQAAAIAKVNTANHWNAPHEAALEVLAQGFIDLKLCKG
                     SLSGVIESGDYKQFYMHRTGHWLGLDVHDAGEYKNKAGAWRQLEAGMTLTVEPGCYIR
                     PADHVPKALWNIGIRIEDDVLVTTAGAEVLTKNAIKTEVDIESIMAKS"
     gene            8205..9377
                     /locus_tag="PKL42_05530"
     CDS             8205..9377
                     /locus_tag="PKL42_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNU66571.1"
                     /translation="MSETMREPIIIVGGGPVGATLALLLAQKGIATTVLEARKKAAAY
                     TESRALALSFGSRKILEKLGVWTALAPHVTAINTIHVSQKGSLGRSILRAQDFNLAAL
                     GYVVSYGALSAALDEVLANTTIVNFVFEASAEAITYHPTHATVTYRQHQFSQSLNSAL
                     VVLADGGRGLDEIAGMVKETKDYGHDALISKVSAELPHNHIAYERFTAHGPMALLPNG
                     ARDFSLVWTGKKAYVNNLLNLSDEAFLALFHAEFGDRVGRFLTVAKRMTFPLKLSQLK
                     TTDTPHLVVIGNAAQTMHPVAGQGFNVGLRDAETLANCIASSAPERLGDEKMLADYRV
                     SRQTDTKNGLLFTDFLVNIFSNDIVCVSGLRSIGLGVFDMVKPIKAHLVSKMSFGK"
     gene            9374..>9770
                     /locus_tag="PKL42_05535"
     CDS             9374..>9770
                     /locus_tag="PKL42_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNU66572.1"
                     /translation="MSTILQKDVVIVGAGLVGLSAAVGLHQAGYSVVLVDRQNPTKLA
                     STDDAWDTRIYAVSPRNAQWLATLGVWQWLPPARVCEIQAMDIFGDMSQKPLSLSAAD
                     ANAENLGFIVESSALMQALLKQIALLGVPT"
BASE COUNT         2558 a         2083 c         2395 g         2734 t
ORIGIN      
        1 gccttgatcg tggtacatgc ataaaaaggc atccgcattt ttgaggttgt tgggggaaaa
       61 catggtgtca gctggcaatg cgccgactaa atcaaagcct tgcgcacgta atttattgag
      121 cacaggattg atggtttcaa tttcttccat gcccaaatag ccgccttcgc ccgcgtgcgg
      181 atttaaacca gcaacataaa ttttaggctg tggtatgcca aatttattga ctaaatcggc
      241 atgcagaatg cggattgtgg tttctaagct tgctaaggtg atggcggctg gcacatcttt
      301 tagcgcaagg tgtgtggtcg ccagcgccac acgcatgccg ccgcccacta gcatcatcac
      361 tacttgtttg gtgttggtga gttccgccaa atattcggta tggccagtaa aggcaatgcc
      421 tgcatcgttg atcacccctt tgtgtacggg ggcggtgacc atggcatcaa acgtgccatt
      481 caggcaacct tgtgcggctg actttagcgt atttaataca taagtactat tgtttgggtt
      541 gagttggccg gcaacaacgg gttctgcgag cgcatgatgc aatacctgca aaccgccgtt
      601 acctaaatgc ggcacttcat ttgcgcaaat gtgatcagca caaatatgca gcggcaaatt
      661 gagcgcttgg gcccgctgtt taagcgcata ttggtctgca atgacgatga tatttgcggc
      721 aaggggtatc tgcgctaact tcacgcataa atcaaggcca atgcccgctg gctcgccagc
      781 ggtaatggca attctagggc gctgcgctgt cacagattag aatttatctt caagtctcag
      841 ttctacataa gcacggtcgc gcaattcgcg aatccagtct tgataggctt cttcgccttt
      901 acgtgcgcgg atttcctggc gtgcttttaa gcgcgccgct tctttggtca tgtcttgccc
      961 gcggcgctca agtacctgaa ttacgtgcca accaaactgg ctacgcacag gtgcgctaat
     1021 ttcattttct ctgagttcgt tcatcgcttt ttcaaactgc ggcacggtgt cacccgggtt
     1081 tacccagcct aaatcaccgc cgttggaagc ggttgaatct tcagagtatt gtcgtgcgag
     1141 gatttcaaat ttttcgccat tatccaaacg ttctttgata ttctccattt tggttttacc
     1201 ttcagcttca gacatgattt ctgaaagctt aatcaggata tgtcgtgcgc gtgtttgttg
     1261 cacaacaaga ggcgagttac cgccacgttt attcgtgagt ttcaatatat ggtaaccatt
     1321 cgggctacgt attggttcag acacctcgcc aatttgtagg ttttttaggg tttctaaaaa
     1381 taaattcggc atttgcgagc cttttttcca gcctaaatta ccgccttcaa gcgcgtttgg
     1441 cgcgtccgag tagctggcgg atacttttgc aaaactcgtt ccagcgctca gtgcttttat
     1501 agcctcttca actttggctt tggctttttg gatttcttct gttgcgcctt cttcaggggt
     1561 gcgaatcaaa atgtgtgaaa tttcaaattc atccaagctc tcaccgctgt tggattggtt
     1621 agttaagtaa ttatccactt cagattcggt cacattcacg cgaccttcga cctcgcgctc
     1681 acgcaggcgc gccagtgtga tttcattgcg aatatcgttt ctgaacttac gcatgctgat
     1741 accgtctcgg gtcagcgctt cagaaaattc tgtcagtgtc agattgtttt ggtctgcaat
     1801 tcgctctatg gttttatcta actgactatc ttcaactttt aagccagttt gcgctgcata
     1861 ttgaatttgt agcgtgtcag agatcagacg ctcaagaatt tgtttacgca acagtgcttc
     1921 atcgggtaat tctgcgcctt gttttttgag ttgcgccgtg acagttgcaa tacgaccttc
     1981 cagttcttgc tcagtaatca cggtttggtc taccaccgcc acaatgcggt ctaatttttg
     2041 aacttcagcc gctactatat tgaatgaagc ggctaaacaa acgtacaaaa atacgcgact
     2101 aaaaaattga atatattttt gcaaaataat cactcgtaat aagattgttg attagagtca
     2161 ggaatcaggc cagaactcac atagccaggc acactacgtt taatgacgga taacggattg
     2221 ttgccaatcg aggctaatcc acccaattct aactgaaaga acaaggcata gttagcatcg
     2281 gccgttgctg tttctacgcg ttggataagc gttctggctt gccagcagcc cgcattgtat
     2341 tctaggccag caatgctttc aatgagttgt ttctcacgaa gtgagtagtt catacgcgca
     2401 acgccgtacc agcccggttt aatcggccat tgtgcagaaa catccacttg gtcaatagaa
     2461 tcacgtctgt agctgtaact caagttaagc gctttacctg gttctgggtt ataacggctg
     2521 gtaatggtgg tacgcactga ttgcgaatca tccgtgttgt attgccagaa ggcatcaagg
     2581 ttgagcgtgg tttttaagtt tgtggtgagg ccagcaatga tgtctgagct attgtttttt
     2641 ctgagctcgg cgccaggcaa tgctactttt tggtctgcaa aataatagcg ctggccaata
     2701 gaggccgata agcgttgtgt gccgctcgca gattcaatta aacgcgaggt gacggagaaa
     2761 ctgagctggt ttgcgttatt aacgcggtca ttgcctgaaa actgattttc actgaataaa
     2821 ctactaaagt ttaaatctga ttcgctggtg tcgaagattg gaatcagtga ttgctttgat
     2881 tctggaatat aagtgtagaa caatcttggt tcaatggttt gggaatagcc gcgtctggca
     2941 attttaaaat cgcggtcaaa aaatagcccg ctatcgacac tgaaaatcgg taacgtgcgg
     3001 ctgatgctgt tttggttatt ggggtcattg ttaaggctgt aattggtgtg gtgaacgcca
     3061 atttttggtg tgagataacc ataagaccgg ttcatgggca agctgatgct cgggtaaacg
     3121 gtaaagcgcg tgccagtgac taaggttggc gcattgttgt tggtgtcaaa agccaccagt
     3181 tgtgtgtaca ggtttgtgtt gaagtcacca aagtaacggt tgcctgtgag cgtcatttgc
     3241 ggcaagcgct catagggata tgaaacttgg tcaagcgttt ggtatttttg cgccagcgca
     3301 ttgaaccgcc atgtttcatc cgcgtaatcc acgttaaact gttgcgcaag gttcacgcgg
     3361 ctggtggtga taatgcgggt ggacatgtca gaaaaatatt ggtcgtcaga gactttttct
     3421 aggctataac ctgccgacca gccacggcca aggttatgct gatgttttaa gttggcatag
     3481 taacgatttt ttccagacag gctatcactt ggtagaaatt cgatattatc aatgccagaa
     3541 aagctgggtt ctaaatatct gaattcgcct tgtaactgaa cgccacgttt actcagtgcg
     3601 cgtgctgcaa aagtggcatc catgttgggt gaaatgttcc aataaaacgg cacggttaac
     3661 tcaaagccgc ttttggatgt agtgccataa gtgggcgcca gtaagcctga tttgcgctga
     3721 ttgttgtatg aaaagctcac ccatggcgtg tagagtattg gcacgccttt aaattcaatg
     3781 tacgcatttt tagcgacgcc tgactctgta aagtcgttga gttcaatgtc tttggccttg
     3841 atgtaccaat catccacccc aggtgcgcaa gtggtgtaac gtgcgccttt gagccttttt
     3901 gtgtcttgac cttcaaacag aattgtctgg gcgtcgccgc gcgctgacga tacacgggtt
     3961 tcaggaatat cttgcgcaat gccagattca atctttgtga cgccatcggt atggttgcga
     4021 tctgcgattg ttttgctgcc atcaagcgta taggtactca cgccgccaat ttgctggctt
     4081 tgtaaattac ctaaactttt agaaaaagcc tctgagctaa tggtgatgag ttgatctttg
     4141 ttttctcgcg cgggtttaaa taaagtaatt gaagcgtctt tcatttcgcc cacatcgtca
     4201 gataactgca tgctgagagc aggcccagtg agtttggcgt tgcccacgtt gatttgcgca
     4261 tttcctttaa catcgagctg ctcgttttgt acatcgtagt caatggtgtc acccaaaatg
     4321 gttttaccat tctggctgat tgatgcgtca ccaattgcgc gcatttttct gtctagatac
     4381 aactcaatct tatcaccatc cacaaccact gatccggcag caatttcagt tgcgctgtca
     4441 ctgctggctt caacaagggg ttcatttgag tgtgtttcat caacgctcac ctcttcagcc
     4501 gccagcgcta tctggctaag cccaagcagc atcatgctaa cacataaaag ggtgcgattt
     4561 tttagcataa gcaatgcaag gtacttgctt ggtgagtgaa aagtgaattt aaatgcacga
     4621 tgtattttag gtggacggtt ttatgttaac gataattttt atcagtgata aaatctcaca
     4681 aatcaggctt tttggcaatg attaaaaaat tgccaacgcg cattatataa cattagaaag
     4741 atagacgtgg atacaagaca gcaagaactt aacgattggc tcacattaac actccctgac
     4801 gcagatttta ccctgacgac ggcttcggca gatgcgagtt tcaggcgcta ctttagagta
     4861 cacacgcaca acggaacgct cattgccatg gatgcgccgc cgccacaaga aaattgcgag
     4921 ccgtttgtgc gcattgccaa actgtttggc gatgcgggct taaatgtgcc taaggtcatt
     4981 gcagaagatt tagaacgagg ctttttgttg cttaatgatt taggcgatga tacttattta
     5041 tcaaaaatta atgcagataa cgcgccaaag ctctatgcgg atgccaacaa tgccttgatt
     5101 aaactgcaac tcgccagtca gcccaatgtg ttacccagct atgatgaagc cttgctgacg
     5161 cgcgaaatgc aattattccc cgattggtat attgcaaaac atttaaacgt aactttagat
     5221 gaaaagcaac aagcggttct caccaacacg tttaatgtgc tgaataaaaa cattctcgcg
     5281 caaaagcaag tctatgtaca ccgagattat cactcacgta acttgatgat gacgcaagac
     5341 aataaccctg gtgtgctgga ctttcaagat gctgtttatg gcgcaattac ttacgatttg
     5401 gtttcactgc tgaaagacgc ttacatcagc tgggatgaag agttgattat tgattggctg
     5461 gtgcgctatt ggcaaaacgc gcgcaaagca ggcttggccg tgccagaaga ttttagcgag
     5521 ttttaccgcg attttgaata catgggcgca caacgtcaca ttaaagtgtt gggcattttc
     5581 gcaaggcttt accatcgcga tggcaaagac ggctatttaa aagatatgcc cctcgtcatg
     5641 gagtatttgc gaaaagtgtg tgaacgttat gtagaactta ggccgatgtt gcgtttgctg
     5701 gatagcttga gtgggcaaga ccaacaccct gatgcgaatt ataaaagttt gctaaaacta
     5761 tgaaagccat gattttggca gccgggcgcg gtgagcgcat gcgcccattg actgaccaca
     5821 cgccaaaacc attactcaag gtgggtggca agccactgat cgtttggcat attgaaaact
     5881 tagcaaaagc gggctttact gaaattatca tcaatcacgc gcacttgggc gagcaaattg
     5941 aagatgcgtt gggtgatgga gcaaagtggg gtgttggcat tcaatatagc cccgaaaaag
     6001 tggctttaga aaccgcaggt ggcattgcca atgcactgcc tttgttaggc gatgcaccat
     6061 ttttagtggt aaatggcgat gtgtttacgg ggttagattt ttctcaatta tttgaaaaca
     6121 cccttcgacc attcgacgag ttcatggctc agggcgaatg ggacaaggat ttgagactaa
     6181 atacaacggt tcgccctgag cctgtcgaag ggcgtgcgca atcaaattac aatcttgccc
     6241 acttggtttt ggtggataat cctccgcagc acccaaaagg tgacttcgct attgaaaatg
     6301 gaatgcttaa aaatgtaggt gagaccatgc tcactttttc aggggtaggt gtttatcatc
     6361 cagatttatt tgccagcatt acgcgcggtg aacctgcaaa actcgcgcca cttttgcggc
     6421 aagccattca agaaaacaaa gccacggcag aatattttaa aggggtttgg cacgatatcg
     6481 gtacgcccga gcgtttaaaa gcgttagatg atgcgctgtt gcgtttgtaa attctgccaa
     6541 agaaaaattg ccaaaatagg ctgaaaatta gcgccgaaaa aatcaataaa acaggcttaa
     6601 aatctcccga gcaaaaaaac atgctctgga agcctttatt tacaaggctt ccagagcatc
     6661 aaaaaaggtt gtgtattaaa atgcgcctta aaacaggttt ttagtatgca tttttttgca
     6721 tagtttaagc ctttagtttt tgtgtaaaaa caatcaagat tgaaccattt gaaacgaaac
     6781 gccacaaaat aaacatcact ttgtgtgatg gcttttgttt atgttgctaa aatgccatat
     6841 tgagtgaatc aaccctatca aaaaagtgaa gtaaacattg gcatgcagac gcaccccaca
     6901 gaagctttag cagaatttaa atcaagacgg caacaactca ttgcacaaat ggttgaaggg
     6961 gttgccatta tccccactgc ggctgaggca atccgcaacc gcgacagcca ttatccttac
     7021 cgattcgaca gttattttta ttacctcacc ggctttaaag agcctgaggc tttgctggtt
     7081 ttagtggcag gtaatgagaa taaaagcatt ttattttgtc gcgataaaga catggagcgc
     7141 gaaatttggg atggctttcg ttacggcgcg gaggctgcta aagcggaatt tggctttgat
     7201 gaagcctaca gttttaatca acttgatgaa atgctgccaa aattactagg caatcagact
     7261 aaattatttt atagcttggg tgcggatgca agctgggacg cgcgcgtgac aggttggttg
     7321 aaccaagtta aatcacaagc gcgtacaggc attagtgcac cagatgaagt gtttgatgtg
     7381 cgtaagcttg tagatgaaat gcggctgtat aaatctgcct atgaaattga cgtgatgcgc
     7441 cgctctgcca acattgccgc cgctgcgcat aaccgcgcca tgcaaaaaac gcgcgcaggc
     7501 aaaatggaat atgaaattga agccgaattt ttgcatgagt tttatcgcaa aggtgcgcaa
     7561 gcgccagctt ataccagcat tattgcgggt ggcgctaatg cctgcacatt gcattacaac
     7621 gccaataatt gcgtgctaaa agatggtgat ttgttgctga ttgatgcggg ctgtgagctg
     7681 gatggctatg ctagcgacat cacacgtact tttccagtta acggtaagtt cagtgcagcg
     7741 caaaaagatg tgtacgaatt ggtgttagcc gcgcaagctg cggccattgc caaagtaaat
     7801 accgcaaacc attggaatgc accgcatgaa gctgcgcttg aagtattagc ccaagggttt
     7861 atagacctaa aactatgcaa aggcagcctt tcaggggtga ttgaaagtgg tgattacaaa
     7921 cagttttaca tgcaccgcac cggccactgg ctgggattag atgtgcacga tgctggtgaa
     7981 tataaaaaca aagcgggtgc gtggcgtcaa ttagaagcag gaatgacgct caccgtagag
     8041 ccaggctgtt atattcgccc agcagaccat gtacctaaag cgctctggaa tattggcatt
     8101 cgtatagaag acgatgtgtt ggtgacgaca gcgggtgctg aagtgctgac taaaaacgcc
     8161 attaaaactg aggtggatat tgaatctatc atggcaaaaa gctaatgtca gaaacaatgc
     8221 gtgaacctat cattattgtc ggtggcggcc cggttggcgc aacgctagcg ttgctactgg
     8281 cacaaaaagg gattgcaacc accgtgctag aagctcgcaa aaaagcggcg gcgtacactg
     8341 aaagccgcgc attagcgttg tcttttggca gccgcaaaat tcttgaaaag cttggcgtat
     8401 ggacggcttt ggcaccgcat gttacggcga ttaacaccat tcatgtgtcg caaaagggta
     8461 gccttggtcg gtcaatattg cgcgcgcaag attttaacct tgctgcattg ggctatgttg
     8521 tctcttatgg tgcattgagt gcagcgctgg atgaagtatt ggctaacact acaatcgtta
     8581 attttgtgtt tgaagccagt gcagaagcca tcacttatca cccaacgcac gctacagtca
     8641 cctatcggca gcatcaattc agtcagtcct taaatagtgc attggtcgta ttagcagatg
     8701 gcggccgtgg cttagatgaa attgcaggca tggtcaaaga aactaaagat tacggccatg
     8761 atgctttaat tagtaaagtg agtgcagagt taccgcacaa tcatattgct tatgagcgct
     8821 ttactgcaca tgggccgatg gccttgctgc caaatggcgc gcgtgacttt tcgttggtgt
     8881 ggacaggtaa aaaagcatac gtgaataact tgttgaatct gagtgacgaa gcgtttttgg
     8941 cgctatttca tgcagaattt ggcgatagag tgggacgttt cttaacggta gccaaacgta
     9001 tgacgtttcc actgaagcta tcgcaattaa aaactaccga tacgccgcat ttggtggtaa
     9061 tcggtaatgc agcacaaacc atgcaccccg tggcgggaca aggctttaat gtgggtttgc
     9121 gcgatgctga gacgttggca aattgcattg ccagcagtgc gcctgaacgg ttgggcgatg
     9181 aaaaaatgct tgcggattac cgtgtaagtc gccaaacgga tactaaaaac ggattgttat
     9241 tcacagattt tctggtcaat attttctcca atgatattgt ttgcgtcagc ggtctcagaa
     9301 gtattgggtt gggcgtgttt gatatggtta aaccgattaa agcacatttg gtcagtaaaa
     9361 tgagttttgg taaatgagta ctatcctgca aaaggatgtt gttatcgtcg gcgcaggttt
     9421 ggttggcctg tcagccgccg tagggttgca tcaggcgggg tattcggttg ttctggttga
     9481 tcgtcaaaat ccaacaaaat tagcatctac tgatgacgct tgggatacca gaatctatgc
     9541 ggttagccct agaaacgcac agtggctggc aactttgggt gtttggcaat ggttgccgcc
     9601 cgcgcgagtc tgtgaaattc aggccatgga tatttttggc gatatgtcac aaaagccgct
     9661 atcgctaagc gcggctgatg ccaatgcaga aaacttaggc tttatagttg aatctagtgc
     9721 cttaatgcaa gcattgctca agcagatagc gttgctaggt gttccaactt
//
LOCUS       DAOQSI010000064         9741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_6334_length_9741_cov_5.852880, whole genome shotgun sequence.
ACCESSION   DAOQSI010000064 DAOQSI010000000
VERSION     DAOQSI010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 9741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9741
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6334_length_9741_cov_5.852880"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PKL42_05540"
     CDS             complement(<1..651)
                     /locus_tag="PKL42_05540"
                     /inference="COORDINATES: protein motif:HMM:NF017078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HNU66573.1"
                     /translation="MAEKHFTGLRAKMLVVVAVGMILLFTLVFFSARKVLYDGYAKLE
                     SNKTLIQVSSAATLLNEQIEQLDGIIAEYAHWDDTYQYMAQPDQRFIESNFTDGTFAN
                     LKVKAILLVTPEGATHYKRGFDYSTGKPWPIPPSLAQAISKEGVFLDASHNKISGFFW
                     TPEGVCIVSASSILPTSGDKKTTRRGTLIMVRHLDQALMEHLDKVVGAKVVFQQKLE"
     gene            822..2078
                     /locus_tag="PKL42_05545"
     CDS             822..2078
                     /locus_tag="PKL42_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HNU66574.1"
                     /translation="MNSLPFELFVGWRYTRAKRKNHFTSFISITSMVGIALGVAVLII
                     VLSVMNGFGTELRNRILGVASHMQVMGINSPLSDWQNVVAQAKTVPQVKAAAPYVMGQ
                     GMLSFGQYAQGAIVRGIVPAQEVQVAELGNKMKVGQLDNLRAGEFGIVLGIDLARYLG
                     AELGDKVMLMTPNGQATPAGMVPRMKQFKLVGVFEMDMKEYDGNLALVHIEDAAKLYR
                     MGDDVSGVRLKLDDPFHASEVSPLLSLALSGQQNYYISDWVDENPNFFAALKMEKRVM
                     FIIMALVVAVAAFNIVSTLIMAVTDKRADIAIMRTLGASPSSIMQIFVVQGALIGVIG
                     TLAGVVLGVLIALNIDVIVPFIEHLFGIQFLDKDVYMITSVPSQLLWSDVLVIMLTSF
                     ALSLLATLYPSWKAARMNPAEALRYE"
     gene            2071..2763
                     /gene="lolD"
                     /locus_tag="PKL42_05550"
     CDS             2071..2763
                     /gene="lolD"
                     /locus_tag="PKL42_05550"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HNU66575.1"
                     /translation="MNNATTKTNNILRCIGLQKTYAGLEVPVLAGIDFSVNAGEQVVV
                     VGASGSGKSTLLHLLAGLDSPSQGEVMLMGKSLKSMTETNKGLMRNQSLGFVYQFHHL
                     LPEFTALENVALPLLIRKLAKREAYQQASEMLAKVGLGHRLEHLPGELSGGERQRAAV
                     ARALVTKPKCVIADEPTGNLDRTTAHQVFDMLLDINQTQGVALVVVTHDLELAAKMQR
                     QYRLVDGLLQPL"
     gene            2798..5212
                     /locus_tag="PKL42_05555"
     CDS             2798..5212
                     /locus_tag="PKL42_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HNU66576.1"
                     /translation="MRLAALNFVLGAWLVQQLTQLPSVPWLVCVWIVALVLLCLLFAP
                     QHQQQSSVNKYSKVFLRGLAALMLGLSWASSVALWRLSDALPHTWEQKTIAIEGVVAS
                     VPEVTERGVRFRFDVEKTLTEHAVVPSHISLNYYAVNQYAKDQQTIEPTATQSNLFNA
                     GERWLLNVRLKRPHGSQNPHGFDFESWALSENIRATGSIKTKAGWKKLDDFVWRPSYM
                     IEHVRARIQQRIASVLANKPYSGVIQALVMGNDNNITPEDWQIFLRTGTTHLMSISGL
                     HITMLSGLAFGLASFLWRRSPKLAIRIPTRKAAVLAGVLVALSYALIAGFSVPTQRTV
                     YMLSVFAVALWSGRQVAISQVLALALLVVVLLDPWAVNAPGFWLSFGAVALIAYALDA
                     RIGESHWFKVAVKTQWAVTIGMLPLLLVMFNQASIISPIANAIAIPVISFVVTPLALL
                     GSFLPVDAPLKWSYFALELCMRVLTWLNQLPLATWQQHTPPVWTLFPALIGVFWLLLP
                     RGMPMRWLGLLGFLPMLFIVPARPMQGDMKITVLDVGQGLSVVVQTSKRTLLYDTGPK
                     FNAQSDAGSRIVVPYLRGEGIGKLDGMMVSHHDNDHSGGLSSILALMPVDWLNSALPE
                     SLALPESLETMQCYAGQIWTWDDVQFEVLHPSLESYDNEKIKDNDRSCVLKITSHAGS
                     MLMTGDIEKNVELALVELDADILKSDVVIAPHHGSKSSSTAAFIEAVAPSASIMTPGY
                     LNRYQHPKPEVVVRYQAAGSQIYRSDYHGALEIHYVKNQPIRFNSYRVQHRRYWHDVF
                     ENSPAP"
     gene            5623..6228
                     /locus_tag="PKL42_05560"
     CDS             5623..6228
                     /locus_tag="PKL42_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNU66577.1"
                     /translation="MWQIILAAGWPIWPLIIASVVALAIIGERAFALRESSVAPQNLL
                     PEVQSWLAAGGVAKETIAKLEQHSPLGKVFASALVNTANSREVTKEAIEETGRAVAHS
                     LERYLSTLGTIATVAPLLGLLGTVIGMVELFGAFTNTGHDIAQFARGISVALYNTAAG
                     IIVAVPAMVAYRYYRSKVDDLLVGMEQQAIKLVEIIHGERK"
     gene            6268..6690
                     /locus_tag="PKL42_05565"
     CDS             6268..6690
                     /locus_tag="PKL42_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNU66578.1"
                     /translation="MNFQRGKRHEELEMNLVPLIDVLLVIIIFLVVSATFSRTSELQI
                     NLPTAEANQAQDKPLTIEVGVDASGKYVVNGKALADTSVSGISAALQAAAQGGKEPTI
                     IINADAKATHQSVIDVMEASRTAGYTHITFATQQPAGN"
     gene            6766..8523
                     /gene="msbA"
                     /locus_tag="PKL42_05570"
     CDS             6766..8523
                     /gene="msbA"
                     /locus_tag="PKL42_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HNU66579.1"
                     /translation="MSKSRRNKEKLPQIANAKVLYLRLFRYAWRYKYVFVTSILSLVV
                     LSASNTGFLALIKQVTDEGFVKKAAGQEIILPLMIFGLMFIRGLAGYISVYSMRTVAR
                     RVVEDFRKEMFARLMVLPVNYFDARSSGALVAKFGYDVERLSTATTRSWLNILRDILT
                     VIGLLGYMLYLDWQLTLVFACILPFIAFYLRQVTPKLKSGAKLVQDSVGSMTKSAEEA
                     IAGQRIVKIFGAQDFELNRFSEIVTNNRRIELRLTRIAGLSSFMVEMFAAIALGLVIY
                     YAISHFTIGEFAAFVGALLMMISPIKHIAAANEDFQIGLAAAQSIFEVMDAAPEQDEG
                     AIEVMRAKGEIEFKNVTLRYENASGVALDNLSFTIKAGEKIALVGRSGGGKTTLVNLL
                     PRFYELQQGVVLLDGIDIRALKLRNLREQFAMVSQDIVLFNDTIFNNIAYGVLRSATE
                     EEVIAAAKAAHAWEFIQQLPLGLQNEIGDRGVRLSGGQRQRIAIARAILKNAPILLLD
                     EATSALDTESEQHVQAAMDALMQNRTSIVIAHRLSTIENADRIMVMEQGKIIEAGTHD
                     ELMKQNQHYAKLYQKQFNT"
     gene            8557..9567
                     /gene="lpxK"
                     /locus_tag="PKL42_05575"
     CDS             8557..9567
                     /gene="lpxK"
                     /locus_tag="PKL42_05575"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNU66580.1"
                     /translation="MRNWFQKQWQTITPWHILLMPLSWLFGAIVYIRRTLYQIGLLKS
                     MHLNVPVIVVGNISVGGTGKTPMVIWLANTLKQQGYRPGIISRGYGGSAKQVTEVFAH
                     SNPLQVGDEPVLITKRTACPMFVAADRVLAGKALLTAHPDCNVIISDDGLQHYRLARD
                     MEIALINSKDGFGNHLLLPAGPLREKVARLQAVDAIVDSANQPNLDLAIKLPPQFNLQ
                     LQGDKFLPVTGEADGQPAQIFKHKKLVAIAGIGNPSRFFNQLSSMGLQFESISFADHH
                     LYAAEDLLKFADKTLVMTEKDAVKCSELGLNEAWFLPVTANVTNLDGVSLIALLQEKL
                     GI"
     gene            9569..>9741
                     /locus_tag="PKL42_05580"
     CDS             9569..>9741
                     /locus_tag="PKL42_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008107784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HNU66581.1"
                     /translation="MDTKLLQILVCPVTKGALIYNKEKNELISKSARLAYPIKDGIPV
                     MLEEEARHLEQDE"
BASE COUNT         2551 a         2050 c         2446 g         2694 t
ORIGIN      
        1 ctccagtttt tgctgaaaaa ccacttttgc acctacaact ttatcaagat gctccatgag
       61 cgcttgatcc agatggcgca ccataataag cgtgccacgg cgagttgttt ttttgtcacc
      121 actggtcggt agaatgctag atgccgaaac aatacagaca ccttcaggtg tccaaaaaaa
      181 tccggaaatt ttgttgtggc tggcgtctaa aaacacaccc tctttgctaa tggcttgcgc
      241 gagcgatggt ggtattggcc acggttttcc tgtggaataa tcaaaccctc ttttgtagtg
      301 cgttgcgcct tcaggggtaa ccagtagaat ggcttttact ttgaggttgg caaatgttcc
      361 atctgtgaag tttgactcaa taaaacgctg atctggctgc gccatgtatt ggtaagtgtc
      421 atcccaatgc gcatattcag caataatgcc gtctagctgc tcaatttgtt cgttcaaaag
      481 cgttgcagca ctactgactt gaatgagcgt tttgtttgat tccagtttgg catagccgtc
      541 gtagaggact ttgcgggcgg aaaaaaagac gagcgtaaac agcagtatca tccctaccgc
      601 taccaccacc aacattttgg cgcgtaatcc agtaaagtgt ttttctgcca ttgaatctac
      661 caggaaagag ttattgcaaa cgtattgtga taacggcaag caattgccgt atctttaatt
      721 tttctgcaaa tatagagtat ctcacatgaa tgcgctaaca caagcggcaa cccacaaaat
      781 gtatttctga aaaattggat aagcctgata gaatgcggct catgaactct cttccatttg
      841 aattgtttgt tggttggcgc tacacccgcg ccaagcgtaa aaatcatttt acttcattta
      901 tttcgatcac cagtatggtc gggattgcct tgggcgtggc tgtgctgatt attgtgctgt
      961 cggtcatgaa tggttttggt acggaactgc gcaatcgtat tttgggtgta gcctcgcata
     1021 tgcaagtcat gggcatcaac agcccgttat ctgattggca aaatgtggta gcgcaggcta
     1081 aaacagtgcc gcaggtaaaa gcggcagcgc cttacgtgat ggggcaaggc atgctctctt
     1141 ttgggcaata cgcgcaaggg gcgattgtgc gcggcattgt gccggcgcaa gaagtgcaag
     1201 tcgctgaact tggcaataaa atgaaagttg gccagttaga taatttacgt gccggtgaat
     1261 ttggcattgt gctgggcata gatttggcgc gttatttagg tgcagagttg ggcgataaag
     1321 tgatgctcat gacgccgaac ggccaagcca ccccagccgg catggtgccg cgcatgaagc
     1381 aatttaaact agttggtgtg tttgaaatgg atatgaaaga gtatgacggc aatcttgcgc
     1441 ttgttcacat tgaagatgcg gcaaaactgt atcgtatggg tgatgatgtt tcaggggtgc
     1501 gcttaaagtt ggacgatcct tttcacgcca gcgaggtttc acccttgctg agtttggcgt
     1561 taagtggcca gcaaaattat tacattagtg attgggttga tgaaaacccc aatttttttg
     1621 ccgcactcaa aatggaaaag cgcgtcatgt tcatcatcat ggcattagtc gtagcggtgg
     1681 cggcctttaa cattgtgtcc acactgatta tggcggtgac agataaacgg gctgatattg
     1741 caattatgcg cacattaggc gccagcccca gcagtattat gcagatattt gtggtgcagg
     1801 gtgcgttgat tggcgtcatc ggcacccttg caggggtagt gttgggtgtg ttaattgccc
     1861 taaatattga tgttatcgtg ccttttatag aacatttgtt tggtatccag tttttagata
     1921 aagatgtgta tatgattacc tcggtaccct ctcaattgct atggtctgac gtgctggtga
     1981 ttatgttgac atcctttgcg ctaagccttt tggcaacgtt atacccgagc tggaaagcgg
     2041 ccagaatgaa ccccgctgag gctttgcgct atgaataacg ctactacaaa aacaaacaac
     2101 attttgcgct gcattgggct gcaaaaaact tatgcagggc tagaagtgcc ggtattggcc
     2161 ggaattgact tttcagtcaa tgcgggtgag caagtcgttg tggtcggtgc gtccggctct
     2221 ggcaaaagca ctttgttgca cctattggca gggcttgata gcccaagtca gggtgaagtc
     2281 atgctgatgg gaaaatcact taaaagcatg acagaaacca ataaaggcct gatgcgtaat
     2341 caatcgctgg gttttgttta tcagtttcat catctattac ctgagtttac ggcgttggag
     2401 aatgtagccc tgcctttgct gattcgcaag ctagccaagc gtgaggctta tcagcaagcc
     2461 agtgaaatgc tggctaaagt gggtttaggg caccgcctgg agcatttgcc gggtgaactt
     2521 tcgggaggcg aacggcaacg ggctgcggtg gcgcgcgcgt tagtgaccaa gccaaaatgc
     2581 gtcattgccg atgaaccaac aggcaattta gaccgaacaa cggcgcatca ggtattcgat
     2641 atgttattag acatcaacca aacgcaaggc gtggctttgg tggtagtaac gcacgattta
     2701 gaattggccg cgaaaatgca gcggcaatat cggttggtcg atgggttgtt acagcctttg
     2761 taaatgcgct aaaaaccgtt agtcgcagct aaaaatcatg agacttgcgg cattaaattt
     2821 tgtgttgggt gcgtggctag tacaacagct gacgcaactg ccaagtgtgc catggttggt
     2881 ctgcgtatgg attgttgcgc tggtcttatt atgcttgttg ttcgccccgc aacatcagca
     2941 acaatcatcc gttaataaat attcaaaagt ttttttacgc gggttggcgg cattgatgct
     3001 tggcttaagt tgggcaagca gtgttgctct ttggcggctg agtgatgcat tgccgcacac
     3061 ttgggaacaa aaaaccatcg cgattgaagg tgttgttgcc agtgtgccag aagtaaccga
     3121 gcgtggcgtg cgctttcggt ttgatgttga aaaaacactg actgaacatg ccgtggtgcc
     3181 aagccacatt agccttaatt actatgcagt aaatcaatac gctaaagacc aacagacaat
     3241 agaacccact gctacgcaat ctaatctatt caatgcgggt gaacgctggt tgctaaatgt
     3301 acgcttaaag cggccgcacg gtagccaaaa tccgcatggg tttgattttg agtcatgggc
     3361 gttgagtgag aatatacgcg ccacaggcag cattaaaacc aaagcgggtt ggaaaaagtt
     3421 ggacgatttt gtttggcgcc cgagctatat gattgaacat gtgcgtgcac gcattcagca
     3481 gcgcattgca agcgtgctcg ctaataaacc ttatagcggt gtcattcagg cgctggtgat
     3541 gggcaacgat aacaatatta cgcctgaaga ttggcagata tttttacgca caggcaccac
     3601 gcatttgatg agcatttcag ggttgcacat caccatgcta tctggcttgg catttggctt
     3661 ggcgagcttt ttatggcggc gtagccccaa gcttgccata cgcattccca ccagaaaagc
     3721 agcggtgctt gcaggcgttt tggtggcgct cagctatgcg ctgattgcag gcttttcagt
     3781 gccaacacaa cgcacggtct acatgcttag cgtatttgct gtggcgttgt ggtctggccg
     3841 acaagttgcg ataagccagg tgttggcgtt ggcgctattg gtcgtggtgc tgcttgatcc
     3901 gtgggccgtc aacgcgcctg gattttggct ttcatttggc gcggtggctt taattgccta
     3961 tgcattagat gcgcgtattg gtgaatcaca ctggtttaag gtcgcggtaa aaacacaatg
     4021 ggcggtaacg attggtatgt tgccattgct actcgtgatg ttcaatcaag cctctattat
     4081 ttcacccatt gccaatgcaa tcgctattcc cgtcatcagt tttgtagtaa cgcccctcgc
     4141 gttactgggc agttttttac ctgtggatgc gccgctaaaa tggtcatatt tcgcgctgga
     4201 gctttgcatg cgtgtgctga cctggctcaa tcaactacct ttagccactt ggcagcaaca
     4261 cacgccacca gtttggacgc tttttccagc gcttatcggg gtgttttggc tattgctgcc
     4321 acgcgggatg ccgatgcgct ggttaggctt gcttggcttt ttgccgatgc tatttattgt
     4381 tccagcacga cccatgcaag gtgacatgaa aattaccgtg ctcgatgtag ggcaaggcct
     4441 aagtgtggtg gtacaaacca gcaagcgcac actgctttat gacacaggcc caaaatttaa
     4501 tgcacaaagt gacgcgggca gccgcattgt tgtgccttat ctgcgaggtg aaggcattgg
     4561 caaacttgat ggcatgatgg tcagccatca tgataatgac cacagcggag gattgtcatc
     4621 aatactcgcg ctgatgcctg tggattggct taatagcgcc ttgcctgaaa gtttagcgct
     4681 accagaaagc ttagaaacca tgcagtgcta tgctgggcaa atctggactt gggatgatgt
     4741 acagtttgaa gtgctgcacc ccagccttga aagttacgac aacgagaaaa taaaagataa
     4801 cgaccgtagc tgcgtgctta aaattacaag ccatgccggc agtatgttga tgacgggcga
     4861 tattgaaaaa aatgttgagc tggcattggt agaattagac gcagatattt taaagagtga
     4921 tgtggtcatc gcaccgcatc acggcagtaa atcttcttca accgctgctt ttattgaagc
     4981 cgtagccccc agcgcaagca tcatgacgcc gggctacctc aatcgctacc agcatcccaa
     5041 gccggaagtt gtggtgaggt atcaggcggc tggcagccaa atttatcgct cagattatca
     5101 cggtgcgctt gaaattcatt atgtgaaaaa tcagccgatt cgctttaaca gctacagagt
     5161 gcaacatcgc cgatattggc atgatgtgtt tgaaaattcg ccagcgccat aaggcgaaat
     5221 gtttaagcat aatattgtgc tttttggtac cattttttgt tgatttaaaa tacacgcatt
     5281 tgcgccgaga aaaattttat gctctggaag cctttattta aaaggcttcc agagcatcga
     5341 aaaaggttgc atgtaaaatt gcttaaaaaa taagcaattt tcgcctgttt tgatgaatgt
     5401 attttctgct gaatttgcgt gaaattttta gctgaattta aaagcgctta agtgattttg
     5461 atgacacgct ttgcgcttaa cttttattga ctaattgttg ttcttgcttg cccaaacgcg
     5521 ggcgacaagc taaagtatcg caagcataag tttcatcgca ttgatggcaa aattaaagtt
     5581 gtttaatcac agtctttttt gattttttgg agttgtaatc tcatgtggca aattatttta
     5641 gcagctggct ggccaatttg gccccttatc attgcttctg ttgtagcgct ggcaatcatt
     5701 ggagagcggg catttgcatt gcgtgaatca agtgtagcac cacaaaatct gctgcccgaa
     5761 gtgcagtcat ggctggctgc tggcggtgtg gcaaaagaaa caattgccaa gttagagcag
     5821 cactcaccgc ttggtaaagt gttcgccagt gctttagtca ataccgcaaa ctcgcgagaa
     5881 gtgaccaaag aagcgattga agaaaccggg cgcgcggtgg cacacagtct tgagcgttac
     5941 ctttccactt tggggactat tgccacggtt gcccccttgt tgggcttgct cggtaccgtg
     6001 attggtatgg tggaactttt tggcgcattt acaaatactg gccacgatat tgcacaattt
     6061 gcccgcggta tttctgtggc tctttataac acggctgcgg gtattattgt tgcggtgccg
     6121 gcaatggtgg catatcgcta ttaccggtca aaagtagatg acttgctggt gggtatggaa
     6181 cagcaggcaa ttaagctggt ggaaatcatt catggtgaac gcaagtaagc gttctggaat
     6241 caacgagtca aaactgaaag aaataggatg aactttcaac gcggaaaacg tcatgaagaa
     6301 ctcgagatga atctcgtgcc gcttattgat gtgctactgg tgattattat atttttggtg
     6361 gtgagtgcta cgttctcgcg caccagtgag ctgcaaatta atctgccaac cgctgaagct
     6421 aatcaggcgc aagacaaacc gctcacgatt gaagtgggcg tggatgcgag cggtaagtat
     6481 gttgtgaatg gtaaagcgct ggcagatacc tccgtttctg gcataagcgc ggcgctgcaa
     6541 gcggccgcgc agggtggcaa agagcccacg attattatta atgcagatgc aaaagcgacc
     6601 catcaatccg tgatcgatgt gatggaagct tcacgcacgg caggctacac gcacatcact
     6661 tttgcaaccc aacagccagc gggtaattaa ataacttaca acttcaacca ctttcaatca
     6721 caaaaagccg cttttgtgat gcataacctt ttattagtat gactaatgtc aaaatccagg
     6781 cgtaataaag aaaaacttcc gcaaattgcc aacgcaaaag tattatattt gcgcttgttt
     6841 cgctatgcgt ggcgctataa gtatgtgttc gtcaccagta ttttatcgct cgtggtgcta
     6901 tctgccagca atacgggctt tttagcgctc atcaaacagg tgaccgacga aggttttgta
     6961 aaaaaagcgg ctgggcagga aatcattttg ccattaatga tttttggctt gatgtttatt
     7021 cgcggccttg caggctacat ctctgtttat tcaatgcgca ccgttgctag gcgcgtggta
     7081 gaagattttc gtaaggaaat gtttgctcga ctcatggtgc tgccagtcaa ttattttgat
     7141 gcgcgctctt caggcgcatt ggtggctaaa tttggctacg atgtcgagcg gctttcaacc
     7201 gccaccaccc gttcctggct taatattttg cgcgacattc tcactgtaat tggtttgctg
     7261 ggatacatgt tatatctcga ttggcagctg acccttgtgt ttgcctgcat tttgcctttt
     7321 attgcctttt atttaaggca ggtcacccct aaacttaaaa gtggtgcaaa gcttgtgcaa
     7381 gacagcgttg gaagtatgac aaaatcggcg gaagaggcga ttgctggcca gcgcatcgtt
     7441 aaaatttttg gtgcgcaaga ttttgaatta aatcgttttt ctgaaattgt caccaataat
     7501 cggcgtattg aattacgttt gacccgtatc gctggcttga gcagttttat ggtggaaatg
     7561 tttgcagcca ttgcgcttgg attggtgatt tattatgcaa ttagtcattt tacaatcggc
     7621 gagttcgcgg cttttgtggg cgcactgcta atgatgattt caccgattaa acatatcgcg
     7681 gcagctaacg aagactttca aatcggactt gctgccgcgc aaagtatttt tgaagtgatg
     7741 gatgccgcgc ctgagcaaga cgaaggcgca attgaggtaa tgcgtgcaaa aggcgagatt
     7801 gaatttaaaa acgtcacctt gcgttacgaa aatgccagtg gtgttgcctt agataatttg
     7861 agttttacca tcaaagcggg tgaaaaaatt gcgctagtcg ggcgttccgg cggcggtaaa
     7921 accactttag tgaatttgtt gccgcgtttt tatgaactgc aacaaggtgt tgtattgcta
     7981 gatggcatcg atattcgggc actcaaactt agaaacctgc gtgaacagtt cgcaatggtg
     8041 agccaagata ttgtgttgtt caatgacaca atatttaaca atattgccta tggtgtgtta
     8101 agaagtgcaa ccgaagaaga ggtcattgct gccgcaaaag ctgcgcatgc gtgggagttt
     8161 attcaacaac tgccgctcgg tttgcaaaat gaaatcggcg atcgtggtgt gcgcttatca
     8221 ggcgggcagc gccagcgcat cgcgattgcg cgtgccattc taaaaaatgc acccatttta
     8281 ttattagacg aagcgacatc agcactcgat acggaatctg aacagcatgt tcaagcagcc
     8341 atggatgctt taatgcaaaa tcgcaccagt attgtcattg cgcatcgctt gagtacgata
     8401 gaaaacgcag accgcattat ggtgatggag cagggtaaaa tcattgaagc cggtacgcac
     8461 gacgaactga tgaaacaaaa tcagcactat gccaagctgt atcaaaaaca gtttaatact
     8521 tagcagtttg tgacttcaat cggcccaaaa tctaacatgc gtaattggtt tcaaaagcaa
     8581 tggcaaacaa tcacgccttg gcatatttta ctcatgccgc tctcctggtt gtttggcgcc
     8641 attgtctata ttcgacgcac actctatcaa attggcttgt taaaaagtat gcacttgaac
     8701 gtgccggtga ttgtggtcgg aaacattagc gtaggtggta caggaaaaac gccgatggtg
     8761 atatggctgg ccaatacgct aaagcagcaa ggttatcgac cagggattat cagccgaggc
     8821 tacggcggta gcgctaaaca agtcacagaa gtatttgcgc atagtaaccc actgcaggta
     8881 ggcgacgagc cagtgttaat taccaagcgc actgcatgcc ccatgtttgt cgctgcagac
     8941 cgcgttttag ccggaaaagc attattaact gcgcatccag actgtaatgt tattattagt
     9001 gacgatggcc ttcagcatta ccgtttagcg cgagatatgg aaatagcttt aattaactca
     9061 aaagacggtt tcggcaatca cttattatta ccagcgggcc ctttacgtga aaaagtagca
     9121 agactgcagg ctgtagatgc aattgtagat agtgcaaatc agccaaatct tgaccttgct
     9181 ataaaattac ccccccaatt taatttacaa ttacagggtg ataaattttt acctgttacc
     9241 ggtgaggctg acggacagcc tgcacaaatt tttaaacata aaaaattagt cgcaattgcc
     9301 ggaattggaa atccgtcccg ttttttcaat caactatcat caatgggttt acaatttgaa
     9361 agcataagct ttgccgatca ccatctgtac gcggctgaag atttattaaa atttgcagat
     9421 aaaacactgg tgatgacaga aaaagatgcc gtaaaatgca gtgaactagg gttaaatgag
     9481 gcatggtttt taccagtgac agcgaacgtg actaatttag atggagtgtc gcttattgca
     9541 ctcctgcaag aaaagttggg aatataaaat ggatacaaaa ttattacaga ttttagtctg
     9601 ccctgtgacc aaaggtgcat tgatttataa taaagagaaa aatgaactga tttccaaatc
     9661 agcacgattg gcttacccaa tcaaagacgg cattcccgtt atgctggaag aagaagcgcg
     9721 ccatttagag caagatgaat a
//
LOCUS       DAOQSI010000065         9739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_6340_length_9739_cov_5.131144, whole genome shotgun sequence.
ACCESSION   DAOQSI010000065 DAOQSI010000000
VERSION     DAOQSI010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 9739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9739
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6340_length_9739_cov_5.131144"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="PKL42_05585"
     CDS             complement(<1..633)
                     /locus_tag="PKL42_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNU66582.1"
                     /translation="MKSTVKLSQRIVPWLALGGLVVLAFMVVAPFFAPIAWAGIIAYV
                     AWPVAMRIRACCGQRDTLAASITTALAALTLLLPLVWLVWVAQLELSNAFPALQNLLT
                     HPPPVPEPLLNIPWLGEWLVQQQASLSSNPQGLSQVIKTWLTTNANNVTTIVGGVGRN
                     FAKLVFVIVILFFFFRDGARILRELRHVLERFIGQKIHGYLHAVGSTTRAV"
     gene            complement(683..1561)
                     /gene="htpX"
                     /locus_tag="PKL42_05590"
     CDS             complement(683..1561)
                     /gene="htpX"
                     /locus_tag="PKL42_05590"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HNU66583.1"
                     /translation="MKRIFYFLATNIAIMLVLSITMRLLGVEPYLNENGLDLRNLLVF
                     SAIMGFGGAFISLAMSKWSAKRMSGAVVIEEPRTPTETWLIKTVRAQADMVGIKMPEV
                     AIFDSPEVNAFATGMTKNSSLVAVSSGLLNSMTKDEAEAVLAHEVSHIANGDMVTLTL
                     IQGVVNTFVMFLSRVIGYVVDKVVFKTERGTGPAFFVTMMIAQFVLGILASIIVMWFS
                     RQREFRADAGAAKLSSSKKMIAALERLKNEQTPSALPEQMAASGISGGVGSGLKKLFT
                     THPSLDDRIAALKNQL"
     gene            complement(1597..1923)
                     /gene="raiA"
                     /locus_tag="PKL42_05595"
     CDS             complement(1597..1923)
                     /gene="raiA"
                     /locus_tag="PKL42_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNU66584.1"
                     /translation="MQLDIQTNGFTLTEGIRSYTTQRLQFALDRNDQQITHVSVHLAD
                     INGPRGGVDKRCKINLTLVGQNNIVIEDTEADLYVAIDRASERCERTLTRRLERSREF
                     VTSSMA"
     gene            2052..2966
                     /gene="nhaR"
                     /locus_tag="PKL42_05600"
     CDS             2052..2966
                     /gene="nhaR"
                     /locus_tag="PKL42_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HNU66585.1"
                     /translation="MVNYKQLHYFWQVARAGSIARASEQLDLTPQTLSGQIGLLEASL
                     NTVLFRRVGRGLELTEAGRLALSYADEIFEVGAEMEQALTNGEIKHLMPFKVGIADVM
                     PKSIVYKLLAPAMALETAIKIVCKEDRLERLLGDLAIHRLDLVLSDRPMPSDINIKGV
                     SHQLVECGVSFFATPALAAQYGQHFPQSLHQAPLLVPGTDTSMRQKLMHWLDTQRIRP
                     KIVGEFDDSALMKAFGQAGIGIFTAPALIDDEVQRQFNVIKLGQIDAITERYYAISAI
                     RRIHHPAVVAISAAAIAETMTEINIKQP"
     gene            3232..5727
                     /locus_tag="PKL42_05605"
     CDS             3232..5727
                     /locus_tag="PKL42_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNU66586.1"
                     /translation="MKKTENKAKSSAPEHFNLMIIGAGPSGLSAAVRAAEHEMSYVLL
                     ESELAPASTVRSYLRGKFVMAEPRGLPLRSPLPFTASLREKVLETWENAIEAHHLNVR
                     YGAKVVDIKRRDHVLEVTLAEATKLTADHVVLAIGVQGNLRKLEIPGGDLPQIQYQLD
                     DPQAYQDETIVVIGGGDSAVENALALTGRNQIIILNRAEDFSNCKESNIHQLMDARKR
                     GALDWLLETRPKLIEANTKGEFPITLFANTPNGVERIPCHRVIARLGALPARPQLEAF
                     GVAFPTPDPAALPSLTSEYESSVPGLYIIGALAGYPLIKQGINQGYEVVEYILGNPVE
                     PADTALLREKFASFCSDRGVDDVLQKIKKSVPLLAMLNTLQLRELVLESNILQPAAGD
                     IIFARNDYSTTFFFIVEGELDVLIDADETSDATLKAGQFFGEMALVSGRRRPGTVVAR
                     TPCVLIETPRRTMQKLINSVPSMRRVLNEVAIKSAVQICIGLSLSEEDLNDLAQHAVL
                     KSYAAGEELFHEGDEADGLYLIQSGSVTVSHMLGGREVVLFYVAAGHYVGEISLVSGD
                     PRYATVRAAVATDAVMIDAARMRDIIARSPEIRSELDARYLKHLQDQEKRQQVESTLQ
                     AGGVTADSATPSSLISFLIQQGVGEATDVLLIDESLCVRCNHCEDACAETHGGTTRLD
                     RDTGPIYANIRVPTSCRHCEHPHCMKDCPPDAIHRAPHGEVYIDDSCIGCGNCQQNCP
                     YDVIQMAVIQDQPQRSLWQMLLGIQPKVLPKADGAKVAVKCDMCKDIPEGPVCVRACP
                     VGAALRVNPEALLSYAGGTASEIVLQDNIAHKD"
     gene            5766..6578
                     /locus_tag="PKL42_05610"
     CDS             5766..6578
                     /locus_tag="PKL42_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66587.1"
                     /translation="MFNYKNYRYFKWAVVVILVAFAAYLLFEPAVGHYGGSWLGYGLG
                     TISALMVFWMAWYGVRKRRYRSSGNTQGWLSAHIYLGTALTVLVTLHTGFRFGLNIHT
                     FAYGMLLIVVVSGFFGNYAYMIYPRLMTENMGEDSLDGLLLRIAETDKLARQIALVMP
                     DDINFTVDRACRETYIGTRLLEQLRGYQEDCPTEKAVKKLTVMGNDLSPEQRKLYREL
                     YSIMVRRQTLVKRVRQELMYRSRLGIWLYFHVPFAIALITAMLVHIIAVFLY"
     gene            6587..8164
                     /locus_tag="PKL42_05615"
     CDS             6587..8164
                     /locus_tag="PKL42_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNU66588.1"
                     /translation="MNCLLIRISLNKRGLPVRSYRTLNAEELTIGRGAECNIHLLDPR
                     IAMHHAVIKRLDDGQTHLVAINGELQVDGLMVSEVALTDGQQAMIGPYLLKVEPAPPD
                     VNISVSLELSHRLPDDFQDLKARTHEPLQGSSSVKRKIALGLFAFITLIFLALPLAQN
                     LSSRLHTTMEELPMGFDRVWSPGHISNAHLHFGSQCFNCHEKLTQQVTDAACLTCHQD
                     TAPHIANAALQKQVFNQKHRFSEGIRCAECHREHKAPVSLTRQDNAKCVECHGNIKAV
                     NAKTDLSNVHDFDKDHPPFKLTFITENQQVERISQTEKERLIEQSGLIFPHSQHVGKV
                     QGPNGMADVRDLTCTNCHHPKGKEMRFSPPTFERDCKTCHRKELKLGSAEKAIYAPHG
                     SVQQVVSAMQTEAPKQAATYLETLKTEGCAYCHEVLETQKEDALPWQIKPLTINHDWF
                     SKAHFNHAKHRTQQCQACHDVETSETSEDVAMPDKAVCLKCHSGNKPKYKRIASTCMS
                     CHEFHDAHATQEALLKK"
     gene            complement(8444..>9739)
                     /locus_tag="PKL42_05620"
     CDS             complement(8444..>9739)
                     /locus_tag="PKL42_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HNU66589.1"
                     /translation="NPNGSGVGRDTVSSGIEGAWTTNPTQWDNGYFYLLFNYDWALKK
                     SPAGAWQWEPINIKEEDKTVDVENPAIRHNPIMTDADMAMVKDPIYREISERFYKNPE
                     YFSEVFARAWFKLTHRDLGPKSRYLGPDVPQEDLIWQDPVPQANYALSDAEIVELKNT
                     LLNSGLSVAELVATAWDSARTFRGSDYRGGANGGRIRLAPQKNWEGNEPARLQKVLNV
                     LEGIQAGLSKKVSMADLIVLAGTAAVEKAAHDAGVNITVPFAPGRGDATAEMTDADSF
                     AVLEPIHDGYRNWLKDDYAVSAEELLLDRTQLMGLTAHEMTVLVGGMRVLGTNHGGSK
                     HGVLTNREGVLTNDFFVNLTDMRYTWKPVGKNRYEVRERKTGAVKWTATRVDLVFGSN
                     SILRSYAEVYAQDDAKEKFVKDFVKAWTKVMNADRFDLK"
BASE COUNT         2623 a         2293 c         2339 g         2484 t
ORIGIN      
        1 cacagcccgt gtggtgctgc ctacagcatg cagataacca tggattttct gaccgataaa
       61 gcgctctaat acgtggcgaa gttcacgtaa aatacgcgca ccatcacgaa agaagaaaaa
      121 taaaatcaca atcacaaaca cgagttttgc aaaatttctg ccaacaccgc ccacaattgt
      181 tgtgacatta ttggcattgg tagtgagcca tgttttaatc acttgcgata aaccctgcgg
      241 gttgctactc aggctggctt gctgctgaac aagccactca cctaaccaag gaatatttaa
      301 taaaggctca ggcacagggg gcgggtgcgt gagtaagttt tgtaatgcgg gaaaagcatt
      361 gcttagttct aactgcgcca cccaaaccaa ccaaaccaaa ggcaacaaaa gcgtgagtgc
      421 tgccaatgcc gttgtgatgc tggcagcaag cgtatcgcgc tggccgcaac atgcgcgaat
      481 gcgcatggcc accggccacg ccacataagc gatgatgcca gcccaggcga ttggcgcaaa
      541 aaagggcgca accaccataa aagccagcac cactaagccg cccagcgcta gccaaggcac
      601 aatgcgctga ctcagtttta cggtgctttt catcttatct ctttgtcagg catcagccat
      661 ccatgcgtaa tcattgcgtg aattacagct gatttttcaa tgccgcaata cggtcatcta
      721 aacttgggtg cgtggtaaat agctttttaa ggccacttcc cacgccacct gagatgccag
      781 aagccgccat ttgttcaggc aaggcggaag gtgtttgctc attttttaag cgctcaaggg
      841 cagcaatcat ttttttgctg cttgagagtt tagccgcacc ggcatctgcc ctaaattcgc
      901 gttggcgtga gaaccacatc acgataatcg aagccaagat gcccaataca aattgtgcaa
      961 tcatcatcgt gacaaaaaag gcggggcctg tgccgcgctc ggttttaaag accactttat
     1021 caaccacata acctatcacg cgtgataaaa acatcacaaa ggtattgacc accccttgaa
     1081 tgagcgtgag agtgaccatg tcaccattgg caatgtgcga gacttcatgc gcaaggactg
     1141 cttcagcttc gtcttttgtc atgctattga gtaggccaga agaaaccgcc accagcgagc
     1201 tgttttttgt catgcccgtt gcaaacgcgt tgacttcagg cgagtcaaaa attgccactt
     1261 caggcatttt aatgcccacc atatcggctt gcgcgcgcac ggttttaatc aaccatgttt
     1321 cggtaggcgt acgcggttct tcaatgacga ccgcgccaga catgcgtttg gccgaccatt
     1381 tagacatggc gagtgaaata aatgcaccgc caaaacccat aatggctgaa aatactaata
     1441 aattgcgtaa atctaagccg ttttcgttga gataaggctc aacccccaat aagcgcatgg
     1501 tgatagataa caccagcata atggcaatat tggtggctaa aaaataaaaa atacgtttca
     1561 tgacgaactc caaaaattta taaaatgagc ctctacttag gccattgaag aggttacaaa
     1621 ttcgcgcgaa cgttctagcc tgcgtgtcag tgtacgctca caacgctcgc tggcacgatc
     1681 aatggccaca tataaatcgg cttcggtgtc ttcaatgaca atattatttt gcccaaccag
     1741 cgtaagattg attttacaac gtttatcaac accaccgcgc gggccgttaa tatcggcaag
     1801 gtgcacgctc acgtgggtaa tttgttgatc attgcggtct agcgcaaatt gcaggcgttg
     1861 tgtggtgtaa ctgcgaatgc cctcagtgag cgtaaaacca tttgtttgaa tatcgagttg
     1921 catatcatgc tccttcaagt taaataaaac agactacatt caaagtgcat gcatcatagc
     1981 gtagttaaca cttcgtaaaa ataaagctta tactgataaa atgttcgtaa aaaactgatg
     2041 attgggtgac gatggtcaat tacaagcaac tgcattattt ttggcaagtg gcccgtgcgg
     2101 gcagtattgc gcgcgcgagt gagcagctcg atttaacgcc acaaactctt agcggccaaa
     2161 ttggtctgtt ggaagcttcg cttaacaccg tattatttcg ccgcgttggg cgcggtttag
     2221 agctaacaga agctgggcgg ctagcgcttt catatgcgga tgagattttt gaagtgggcg
     2281 ctgaaatgga gcaggcatta accaacggtg aaataaaaca cctgatgccg tttaaagttg
     2341 gcatcgccga tgtgatgcca aaatcaatcg tctataaatt gcttgccccc gccatggcgc
     2401 ttgaaaccgc catcaaaatt gtttgtaaag aagatcgatt agagcggcta ctcggagact
     2461 tggcaattca ccgcttagac ttggtgttat cagaccggcc gatgccgtca gacatcaaca
     2521 tcaaaggggt tagtcaccaa ttggtggaat gtggcgtgag tttttttgcc acgcccgcat
     2581 tggccgcgca atatggccaa cattttccgc aaagcttgca tcaggcgccg ttgctcgtcc
     2641 ctggcaccga taccagtatg cgtcaaaaac tcatgcactg gcttgatacg caacggataa
     2701 ggccaaaaat tgttggcgaa tttgatgaca gtgctttaat gaaagccttt ggccaagcgg
     2761 gcattggtat ttttactgca ccggctttaa ttgatgatga ggtgcaacgc caatttaatg
     2821 tcatcaaatt aggccaaatc gatgccataa ctgagcgata ctatgccata tcagccatcc
     2881 gtcgtattca tcacccagcg gtggtagcga ttagcgcggc tgcaattgcc gaaaccatga
     2941 cagaaatcaa cataaaacaa ccataattgc gtgataattt actatggtga tgatgcagcc
     3001 gtcagtaaaa ttgcattgtg aagataggcg ttaagtgtga gaaaatggcg gcattagctt
     3061 aggatttaaa aaccttaaag gcgcatataa aaaggtgtta aaaaaagcgt tttttagccc
     3121 gagaaaaaaa tgatgctctg cgagccttta tttacaaggc tcgcagagca tccaaaaagg
     3181 ttatatgcaa aacaagcaca taaaacaaca tgttgagatg gtaatttttt agtgaaaaaa
     3241 actgaaaata aagcaaaaag tagtgcgcca gaacacttta atctgatgat tattggcgcg
     3301 ggccccagtg gcttgtcggc agcggtgcgt gctgccgagc atgaaatgtc ttatgtgttg
     3361 ttagagtctg aacttgcccc agccagtacg gtgcgtagtt atcttcgcgg taagtttgtg
     3421 atggccgagc cgcgcggttt gccattacgc agcccattgc cgtttacggc ttcgttgcgc
     3481 gaaaaagtgt tagaaacatg ggaaaacgcc atagaagcgc accatctcaa tgtgcgctac
     3541 ggggccaagg tggtagatat caagcgccgc gatcatgtgc ttgaagtgac gctggcagaa
     3601 gctacaaaac tgactgctga tcacgtggtg ctggccatag gcgtgcaagg caatttgcgc
     3661 aagctggaaa ttccaggcgg tgatttaccg caaattcaat atcaacttga tgacccgcaa
     3721 gcttaccaag atgaaaccat tgttgttatc ggcggcggcg atagtgcggt ggaaaatgcg
     3781 ttggcgctga ccgggcgcaa ccagatcatt attttaaatc gtgctgaaga ttttagtaat
     3841 tgtaaagaaa gcaacatcca ccaattgatg gacgcgcgca agcgcggtgc gctcgattgg
     3901 ctgttagaaa ccagaccaaa actcatcgag gcgaacacca aaggtgagtt tccaatcacc
     3961 ttatttgcca atacgcccaa tggcgttgag cgtattcctt gccatcgtgt gattgcccgt
     4021 ttaggcgcat tgccggcacg cccgcaactt gaagcgtttg gcgtggcttt tccaacacca
     4081 gacccggctg cactgccttc gctcacttca gaatatgaat ctagcgtgcc agggctttat
     4141 attattggcg cgcttgcagg gtatccgctg attaagcagg gcatcaatca agggtatgag
     4201 gtcgttgaat acattttagg taacccggtt gaacctgccg ataccgcgct gctacgcgag
     4261 aaattcgcca gcttttgttc agaccgcggt gtggatgacg tactgcagaa aattaaaaaa
     4321 agtgtgcctt tattggcgat gcttaatacg ctgcaattac gcgaattagt gcttgaaagt
     4381 aatattttac agcctgcggc aggcgatatt atttttgcgc gcaatgacta cagcaccaca
     4441 tttttcttta ttgtggaagg cgaattagac gttttaattg atgccgatga aacatcagac
     4501 gcaaccctga aagcgggcca gttttttggt gaaatggcgt tagtttcagg ccgtcgcaga
     4561 cctggtacgg tagttgcgcg cacgccctgt gtgctgatag aaacacccag acgcaccatg
     4621 caaaaattga ttaactccgt gccgtccatg cgccgtgtat tgaatgaagt ggccattaaa
     4681 tcagccgtgc aaatatgcat agggttgtcg ctcagtgaag aagatttaaa cgacttagcg
     4741 caacatgcgg tactgaaaag ttatgccgca ggtgaagagc tttttcatga gggcgatgaa
     4801 gccgatgggc tgtacctaat acaaagtggt tcagttacgg tttcgcatat gttgggcggc
     4861 agagaagtgg tgttgtttta cgtggcggca ggacattatg tgggcgaaat atcgctggta
     4921 tcaggtgatc cgcgttatgc cacggtgcgt gcagccgttg caaccgatgc cgtaatgatt
     4981 gatgccgccc gcatgcgcga tattattgcg cgcagcccag aaattcgcag tgagcttgat
     5041 gcgcgctacc tcaagcattt gcaagaccag gaaaaacgtc agcaagttga gtcaacgctt
     5101 caggccggtg gggtgacggc agacagtgca acgccatcaa gtttgatttc atttttaatt
     5161 cagcaaggcg tgggtgaggc gacggatgtt ttgttgatag acgaatcgct ttgtgtgcgc
     5221 tgcaaccatt gtgaagatgc gtgtgcagaa acgcatggcg gcaccacgcg gctagatcgt
     5281 gataccgggc cgatttatgc aaatatacgt gtgcctactt catgcaggca ttgtgaacat
     5341 ccgcactgca tgaaagattg cccgcccgat gccatacacc gcgcaccaca tggcgaagtg
     5401 tatattgacg acagctgcat tggctgtggc aactgccagc aaaattgccc ttacgatgtg
     5461 attcaaatgg cagtgattca agatcagcct cagcggagct tgtggcaaat gctattgggt
     5521 attcagccaa aagttttgcc caaagcagac ggcgctaaag tggcagtaaa atgcgacatg
     5581 tgtaaagata taccagaggg gcctgtgtgc gtacgcgcct gcccggtggg cgccgcgttg
     5641 cgggtgaacc cagaagcgct gttaagttat gccggcggca ccgcaagtga gattgtcttg
     5701 caagacaata tcgcccacaa agattagcct ataaaaaaga accagcgccc atgcagcata
     5761 acaacatgtt taattataaa aattatcgct attttaaatg ggcggtggtg gtcattttag
     5821 tcgcctttgc cgcgtattta ttgtttgaac cagcggtcgg acattatggc ggcagttggt
     5881 taggctatgg cttaggcaca atttcggcgt tgatggtgtt ttggatggca tggtacgggg
     5941 tgcgtaaacg tcgctaccgt agcagcggca atacgcaagg ttggctttct gcgcatattt
     6001 atttgggcac cgcgctcacc gtattggtca cccttcatac gggctttcgt tttggtttga
     6061 atattcatac ctttgcctat ggcatgctgc tgattgtggt tgtcagcggc ttttttggca
     6121 attatgccta catgatttat ccgcggctaa tgaccgaaaa catgggcgaa gacagccttg
     6181 atggtttgct tttgcgcatt gctgaaaccg ataaactagc gagacaaatc gcgctggtga
     6241 tgcccgatga tattaacttc acggtggatc gtgcctgccg tgaaacttat atcggcaccc
     6301 gattgctgga acaattaaga ggctatcaag aagattgccc aactgaaaaa gcagtaaaaa
     6361 aactcacggt gatgggtaat gatttgtcgc cagaacaaag aaagctctat cgtgaactat
     6421 attccattat ggtacgccgc caaaccttgg ttaagcgtgt gcgccaagaa ctgatgtaca
     6481 ggtcacggtt aggcatttgg ttgtattttc atgtgccgtt tgcgattgca cttatcaccg
     6541 ccatgctggt gcatattatt gctgtatttt tgtattgata accatcgtga actgcctgtt
     6601 aattagaatt tctctcaata agcgcggcct gccagtgcgc agctaccgca ctttaaatgc
     6661 ggaagaatta accatcggcc gtggcgcaga atgtaatatt cacttattag acccgcgcat
     6721 tgccatgcac cacgccgtaa ttaagcgctt ggatgatgga caaacccacc ttgtggccat
     6781 caatggtgag cttcaagtag acggtttaat ggtgagcgaa gtggccttga ccgatggcca
     6841 gcaggccatg ataggcccgt atctgttaaa ggttgagcct gcaccgccag atgtgaatat
     6901 ttcggtttca ttagagttgt cacaccgctt acccgatgat tttcaggacc taaaagcccg
     6961 tacccacgaa ccgctgcaag gctcatcgtc ggtcaagcgt aaaatcgctt taggcttatt
     7021 tgcattcatc accttaattt tcttggcgtt gccattggcg caaaatttat cttcccgatt
     7081 acataccacc atggaagagc tgccaatggg gtttgatcgg gtatggagtc ctggccatat
     7141 ttcaaacgcc cacctgcatt ttggctcgca atgctttaat tgccatgaaa aactcacgca
     7201 gcaggtgact gatgccgcgt gtttaacttg ccatcaagat accgcgccgc acattgcaaa
     7261 cgctgccttg caaaaacaag tgtttaatca aaaacatcgt ttttcagagg gcattcgttg
     7321 tgcagagtgt catcgtgaac ataaagcgcc ggtatcgctc acgcgccaag ataacgctaa
     7381 atgtgtggag tgccatggca atatcaaagc ggtgaatgcc aaaacagatt taagcaatgt
     7441 gcatgatttt gacaaagatc atccgccgtt taagctaaca tttattacgg aaaatcaaca
     7501 ggttgaacgc atttcacaaa cagaaaaaga gcgcttaatt gagcaatctg gcttaatttt
     7561 tccgcattcg caacatgtgg gaaaagtgca gggccccaat ggcatggccg atgtgcgcga
     7621 tttaacctgc acaaattgcc atcaccctaa aggcaaagaa atgcgattta gcccaccaac
     7681 ttttgagcgc gactgcaaaa catgccatcg caaagaactc aaattgggta gtgccgaaaa
     7741 agccatttat gcaccgcatg gcagtgtgca acaggtcgtg agtgccatgc aaacagaagc
     7801 ccctaaacag gcggctactt atttggagac gctcaaaaca gaaggctgcg cgtattgcca
     7861 tgaagtgtta gaaacgcaaa aggaagatgc gctgccttgg caaattaagc ccctgacaat
     7921 caaccatgat tggtttagca aagcgcactt taatcatgca aaacaccgca cacaacaatg
     7981 ccaagcctgc catgacgtag aaacctccga aacgagcgaa gacgttgcca tgccggataa
     8041 agcggtgtgt ttaaagtgcc actctggtaa taaaccaaaa tataagcgca ttgccagtac
     8101 gtgcatgtcg tgccacgagt ttcatgacgc gcatgcaaca caagaagctt tactgaaaaa
     8161 ataagcgttt gcttggttga attaaagaca cgccgcacac aaattttaag cggcttaatc
     8221 gcctaaaatt acacgcaacc ttttttgatg ctctgcgagc cttataaata aaggctcaca
     8281 gagcattatt ttttcctcgt acaaaattag gctttttttg aatgtatttt aaagccacac
     8341 ggcgctgttt tttcggatca gaaagcatga taaaaaaccc gccacattta acaattaaat
     8401 gggcgggttt ttcaggcgaa ccgtgcgtta agaatccgcg cgattatttc aaatcaaaac
     8461 ggtccgcatt catcactttg gtccaagctt tcacaaagtc ttttacaaat ttctcttttg
     8521 catcatcttg cgcatacact tctgcataag aacgcagaat cgagtttgag ccaaagacta
     8581 aatcaacgcg tgtagctgtc cacttcactg cgccagtttt acgttcgcgc acttcatagc
     8641 ggttttttcc aacgggtttc catgtgtagc gcatatccgt gaggttcaca aagaaatcgt
     8701 tcgttaaaac accttcacga ttcgtcaata caccgtgttt gcttccacca tgatttgttc
     8761 ccagtacgcg catgccgccc actaaaacgg tcatctcatg cgcggttaag cccatcaact
     8821 gggtgcggtc gagcaatagt tcttctgccg agaccgcgta gtcgtctttc agccaattgc
     8881 gatagccgtc atgaattggt tctagcacag caaaagaatc tgcatcggtc atttctgcgg
     8941 tcgcgtctcc acggcccggt gcaaatggca cggtgatatt tacgccggca tcatgtgcgg
     9001 ctttttccac cgcggccgtg cctgcaagta caatcaaatc tgccatgctg acttttttgc
     9061 tcaggccagc ttgaatgcct tcaagtacat tcagcacttt ttgcagtctg gctggctcat
     9121 tgccttccca gttcttttgc ggcgccaagc gaatacgccc accattagcc ccaccgcgat
     9181 agtctgagcc gcggaaagtg cgtgcactat cccatgcggt cgccaccaat tcagcaacac
     9241 ttaaaccact gttcaaaagt gtgttcttga gttcgacaat ttccgcatca cttaaggcat
     9301 aattggcttg tggaacaggg tcttgccaaa tcaagtcttc ttgtggcaca tcaggaccta
     9361 aataacggct ttttggccct aaatcgcgat gcgttaattt aaaccatgca cgcgcgaata
     9421 cttctgagaa atactccgga tttttgtaga aacgttcaga gatttctctg taaatagggt
     9481 ccttcaccat cgccatatcc gcatcggtca taatcgggtt gtggcgaatc gccgggtttt
     9541 caacatccac ggttttatct tcttctttaa tgttaatcgg ctcccattgc catgcaccag
     9601 ctgggctctt tttcaatgcc caatcgtaat taaaaagtag atagaagtag ccgttatccc
     9661 attgcgttgg gttggtcgtc caagcgcctt caatcccgct tgataccgta tcgcggccaa
     9721 caccgctgcc attcgggtt
//
LOCUS       DAOQSI010000066         9729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_6354_length_9729_cov_4.679657, whole genome shotgun sequence.
ACCESSION   DAOQSI010000066 DAOQSI010000000
VERSION     DAOQSI010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 9729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9729
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6354_length_9729_cov_4.679657"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..659
                     /gene="grpE"
                     /locus_tag="PKL42_05625"
     CDS             156..659
                     /gene="grpE"
                     /locus_tag="PKL42_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNU66590.1"
                     /translation="MQEENQPEVAAEYVEPTEITAEARIAELEAALEEAKASVLYVKA
                     EGENIRRRAMDDIDKARKFALEKFSNELLAVKDSLDAALLIETADVQSYKDGVQLTAN
                     QLTSVFEKFNIAEINPVGEKFDPNKHQAISMQENSGEPNTVFNVLQKGYTLNDRVLRP
                     ALVMVAK"
     gene            793..2721
                     /gene="dnaK"
                     /locus_tag="PKL42_05630"
     CDS             793..2721
                     /gene="dnaK"
                     /locus_tag="PKL42_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNU66591.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGGKPRVIENAEGTRTTPSIIAYQE
                     DGEILAGAPAKRQAVTNPKNTLYAVKRLIGRRFEEKEVQKDIALMPYTIAKADNGDAW
                     VEVRGQKMAPPQISAEVLRKMKKTAEDYLGEEVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLEVKRIINEPTAAALAFGLDKQEGDRKIAVYDLGGGTFDVSIIEISNIDGEHQFEV
                     LSTNGDTFLGGEDFDNRLIDFLADEFKKDNGGLDLRNDLLAKQRLKEAAEKAKIELSS
                     SAQTEVNLPYITADATGPKHLVVKITRAKLESLVDDLIERTIGPCKTAIKDAGVKIED
                     ITDVILVGGQSRMPKVQEKVKEIFGKEPRKDVNPDEAVAVGAAIQGGVLQGDVKDVLL
                     LDVTPLSLGIETLGGVMTKLIKKNTTIPTKASQVFSTADDNQNAVTIQVLQGEREMAS
                     ANKSLGQFNLSDIPPAPRGMPQIEVTFDIDANGILHVSAKDKATGKENKITIKANSGL
                     SEEEIKKMEEDAIKYADEDRKLRELVDARNAADGMVHSVKKSLTEHGDKLDAAEKEAI
                     ETALKDVEEAMKSDDKATIEAKTEALTTASQKLGEKVYAAQQAEANTESAQPSAEGEK
                     TVDGDVVDAEFEEVKKDK"
     gene            2845..3969
                     /gene="dnaJ"
                     /locus_tag="PKL42_05635"
     CDS             2845..3969
                     /gene="dnaJ"
                     /locus_tag="PKL42_05635"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNU66592.1"
                     /translation="MAAKKDYYEVLGVNKDASEEDIKKAYRKLAMKYHPDRNPDNPKA
                     EEQFKEAKEAYEMLSDDQKRAAYDQYGHAGVDPSMGAGAGGFGAGGFSDAFGDIFGDI
                     FGSRAGGQRNNVYRGADLRYNMEISLEDAAKGTETKIRIPVQTTCETCKGSGARPGTQ
                     PVTCTTCGGHGQVRMQQGFFSVQQTCPKCHGSGKMIKDPCPTCHGAGRTKQNKTLSVK
                     IPAGVDEGDRIRLTGEGEAGVNGGPTGDLYVVVHLKEHNVFQREGANLHCEMPISFST
                     AALGGEIEVPTLDGSARMKVPAETQTGAVFRLRGKGVKPLRQSEHGDLMVHVVVETPV
                     KLTEQQKELLRQFETSTQADAGKHSPKNKSWMDKAREFFG"
     gene            4193..4894
                     /locus_tag="PKL42_05640"
     CDS             4193..4894
                     /locus_tag="PKL42_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66593.1"
                     /translation="MKIISWNCNGALRKKLEPISAFNADIYIIQECEDRAKINDKNYQ
                     SWAGQYLWYGHNKNKGVGVFAKNGFSLTPLPLESGRLEYFLPFKVNESFTFLAVWTRE
                     ANSPTFKYIGQMWKYLQLHKNQLKSDKTVIIGDLNSNVRWDAWDRWWNHSDVVRELEG
                     IGICSLYHHIYKEDQGQESKPTFYLQRNIQKPYHIDYAFISKEMAPSASLSVGEKEIW
                     LEHSDHMPLSVHIYA"
     gene            5029..5697
                     /gene="creB"
                     /locus_tag="PKL42_05645"
     CDS             5029..5697
                     /gene="creB"
                     /locus_tag="PKL42_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HNU66594.1"
                     /translation="MPHILIIEDEPAIAETLIYAFKEAGIATTHKMLGGEATPALAQQ
                     AIDFVVLDVGLPDMTGFDVCKKIRETSQVPILFLTARNHEIDRIVGLEIGADDYVTKP
                     FSPREVVARVKAILRRSEPQANHHKNHTFMMDAQANRIQYRGQYLELTRYEFLLLKTL
                     ISQPQRVYSRNQLMDIVWANAENTLERTVDAHIKTLRAKLREVDAATEAIVTHRGMGY
                     SLLA"
     gene            5701..7164
                     /gene="creC"
                     /locus_tag="PKL42_05650"
     CDS             5701..7164
                     /gene="creC"
                     /locus_tag="PKL42_05650"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF008312.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HNU66595.1"
                     /translation="MKISLKIFLGYFLLVGLAAWFVLNVFVDEVKPGVRQAMEDALVD
                     TANVLAELATDDIKNGTIQTGNFAQSLAHYQARSRQSNPNNANIWGINKQTADYRVYI
                     TDAKGMVIYDSANLALGQDYSRWNDVYLTLRGKYGARSSLVVPNDENSDTIMYVAAPI
                     KVGNQIIGSLTVAKPNRALLPFIERAQQKIIRWGALLLVLSLIIGTLFTWRFTRKINQ
                     LRDYAMRVSQGQKATPPTSSNDELTELALAMQTMRVELDGKQHVQDSIQHLTHELKSP
                     MTAIQGALELISPNMPADAQAHFLAQIKVQSTRVQNIIQNMLGLAALEHQQQLTHLTH
                     INLADLIRTQMAHLAEKSTARNVQFQLTEEHAVSLRAEAFLLGQAIYNVLDNALDFSP
                     ENSHIQIHISQQQHQAHIRIQDQGTGIPDYALTKIFDKFYSLPRPQNAPNGGQKSTGL
                     GLNFVQEVIKLHLGEIHIENNSASQGVTVSILLPLNI"
     gene            7476..8900
                     /gene="creD"
                     /locus_tag="PKL42_05655"
     CDS             7476..8900
                     /gene="creD"
                     /locus_tag="PKL42_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HNU66596.1"
                     /translation="MNRAFIFKVIGIFLLTIVMSWAVMYINSLIKERQYRQEEVKLQI
                     ASSSAGAQTIIGPVLAIPYIEEYEETITENKIKKVEKRKAEYVVYALPEDLQLSGGFT
                     NEYKKLGIYKALMYQLGGKVKGTFNLGADFKITPQHAGAKITMLPAYVSMGISDTRGI
                     NGKPALTLNHEVYAFAQGTKLPVLGNGIHAELGKLDVVASKTLNFEFQLNLRGMENFN
                     FTPIADDNKIALQSSWQHPHFNGSFLPTHHITPAGFEAKWAVSSLSSSNQTKLLNMLE
                     QRWNENSAASAAATASRDSAYPAAAQTAYHNSNLESLAIGFVEPINVYSQSDRATKYG
                     LLFIGLTFAGFFIYEILKQLRIHPAQYTLVGLAMALFYLLLTSLSEHISFALSYLTAS
                     LACVALLGYYLTHVLKSKSHGLLFAGLLTALYGALYGILASEDNALLMGALLVFGLLA
                     VTMVITRKVDWYQISSKENMQINA"
     gene            complement(9060..9308)
                     /locus_tag="PKL42_05660"
     CDS             complement(9060..9308)
                     /locus_tag="PKL42_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66597.1"
                     /translation="MNKTALLLAVQAALDGDWDKSHKIAQDYSDATANWIHAVLHKIE
                     GDAWNSKYWYARTAGKHYEDFGDANAELKTIQNYLNQT"
     gene            complement(9305..9580)
                     /locus_tag="PKL42_05665"
     CDS             complement(9305..9580)
                     /locus_tag="PKL42_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNU66598.1"
                     /translation="MLIDVVQDCWFLYLLECKNGAYYAGITNDVEARFTAHLSGKGAR
                     YTRANPPLKVLATKPYAGRSSASVAEAQLKKLPRHKKLAFFEVLPEA"
BASE COUNT         2958 a         2131 c         2199 g         2441 t
ORIGIN      
        1 cacaaaaaat cctcgggcgc atttatgcat aaaaaggtgt ttaaatcacg ctcaaaaaat
       61 tcgtcatttt tatctatttc cctcttgaaa ttggggaaaa gttacccaca ttaattgaaa
      121 cttttattaa aaaataattt gagcgaggaa aatccatgca agaagaaaac caaccagaag
      181 tagcggctga gtatgttgag ccaactgaaa ttacggctga agcgcgcatt gccgagctag
      241 aagccgcgct tgaagaagct aaagccagcg tgctttatgt aaaagctgaa ggtgaaaata
      301 ttcgccgtcg tgcgatggat gatattgata aagcccgtaa atttgcctta gagaaattct
      361 ccaatgaatt attagcggtg aaagatagcc tggacgctgc gctgctgatt gaaaccgctg
      421 acgtgcaaag ttataaagac ggcgtgcaac ttacggcaaa ccaactcacc agcgtgtttg
      481 aaaagttcaa cattgctgaa attaacccag tgggtgaaaa gtttgacccc aataaacatc
      541 aggcaatctc aatgcaggaa aatagcggcg agccaaacac agtatttaac gtgctacaaa
      601 agggttacac cttgaacgac cgcgtgctgc gccctgcttt ggtgatggtg gcaaaataat
      661 atcgttatct catattcagc cacttgaatt taaaaaaaca atcaccatct agctattaag
      721 gttaaacaac aaactgcttt aaaagttgaa ttaaagcggt taatcaaaat ttaaagtaaa
      781 aggaaattaa acatgggcaa aattattggt attgacttag gtacaacaaa ctcttgcgtt
      841 gcagtcatgg aaggcggcaa accacgcgtg attgaaaacg cggaaggcac acgcacaacc
      901 ccttctatta tcgcctatca agaagatggc gaaatcttag ccggcgcgcc tgcaaaacgc
      961 caagcggtga ccaatccaaa aaacacactt tacgcagtaa aacgcttaat cggccgccgt
     1021 tttgaagaaa aagaagtgca aaaagatatt gctttgatgc cttacaccat cgctaaagcc
     1081 gataacggcg atgcatgggt ggaagtgcgc ggccaaaaaa tggcaccgcc gcaaatttct
     1141 gctgaagtgt tgcgcaaaat gaaaaaaacc gctgaagatt acttaggcga agaggtgaca
     1201 gaagctgtga ttacggtgcc agcttacttt aacgacagcc aacgccaagc cactaaagac
     1261 gctggccgta ttgcgggctt ggaagtaaaa cgtatcatca atgagcccac tgctgctgca
     1321 ttggcgtttg gtttagataa acaagaaggc gaccgcaaaa ttgcggtgta tgacttaggc
     1381 ggtggtacgt ttgacgtgtc tattattgaa atttcaaaca ttgatggcga acatcaattt
     1441 gaagtacttt caaccaatgg cgatacattc ctaggcggcg aagactttga taaccgtttg
     1501 attgactttt tagcggatga gtttaaaaaa gataatggcg gcttagattt acgcaatgat
     1561 ttattggcaa aacaacgctt aaaagaagcg gcagaaaaag ccaaaattga attgtcttca
     1621 agcgcacaaa cagaagtaaa cttgccatac atcacggcag atgccactgg cccaaaacac
     1681 ttggtggtaa aaatcacccg cgcaaaattg gaatcattag tggatgattt aattgagcgc
     1741 accattggcc catgcaaaac agccattaaa gatgctggcg ttaaaattga agacatcacc
     1801 gatgtgattt tggtgggcgg tcaaagccgt atgccaaaag tgcaagaaaa agtgaaagaa
     1861 atctttggca aagagccacg taaagacgtt aaccctgatg aagccgtagc cgtgggcgct
     1921 gctattcaag gtggcgtatt gcaaggtgat gtaaaagacg tgctgttgct agatgttaca
     1981 ccgttatcac ttggcattga aacacttggc ggcgtaatga caaaactcat caagaaaaac
     2041 accacgattc ctaccaaggc atcacaagtg ttttcaacgg cggacgacaa ccaaaacgca
     2101 gtgaccattc aagtattgca aggtgagcgt gaaatggctt ctgccaacaa atcacttggc
     2161 caatttaatt tgagcgatat tccaccagcg ccacgtggca tgccgcaaat tgaagtgacg
     2221 tttgatattg atgccaacgg tattttgcat gtttcagcaa aagacaaagc cactggcaaa
     2281 gaaaacaaaa ttaccatcaa ggcaaactct ggcctaagtg aagaagaaat caagaagatg
     2341 gaagaagatg ccattaaata tgccgatgaa gaccgcaaat tgcgcgaact ggttgacgca
     2401 cgtaacgcgg ctgacggcat ggttcactca gtgaaaaaat cactcactga acatggcgat
     2461 aagctagatg ctgctgaaaa agaagccatt gaaacagctt taaaagatgt tgaagaagcg
     2521 atgaagtctg atgacaaagc aaccattgag gctaaaacag aagcgttaac taccgcctca
     2581 caaaaacttg gtgaaaaagt ctatgccgcg caacaagcag aagccaacac tgaaagcgca
     2641 caaccaagcg ctgagggcga aaaaacagtg gatggcgacg tagtagatgc agagtttgaa
     2701 gaagtgaaaa aagacaaata agtaagacgt tatttgtcag tcaatggtcg ttagtatctt
     2761 aaaatgatac taacgacttt tgcactcatg acgaatgatg attaactggt aactcaagac
     2821 cacctaaatg aacgactaaa aattatggca gctaaaaaag attactacga agtgctaggc
     2881 gtaaataaag atgcctcaga agaagacatc aaaaaggctt atcgcaaatt agcgatgaag
     2941 tatcaccctg accgcaaccc agataaccca aaagcagaag agcagtttaa agaggcaaaa
     3001 gaagcctacg aaatgctttc tgacgaccaa aaacgtgccg cttacgacca atacggccac
     3061 gcaggtgtag acccaagcat gggtgctggc gcaggtggct ttggcgcggg cggttttagt
     3121 gatgcctttg gcgatatttt tggtgatatt tttggcagcc gtgcaggcgg ccagcgcaat
     3181 aatgtgtacc gtggcgctga tttacgctac aacatggaaa ttagcctaga agatgccgcc
     3241 aaaggcactg aaaccaaaat tcgcattccg gtgcaaacca cctgtgaaac ctgtaagggc
     3301 tcaggcgcgc gccctggcac gcaacccgtt acttgcacca cttgtggcgg ccacggccaa
     3361 gtgcgtatgc agcaaggctt tttttcagta cagcaaacct gcccaaaatg tcatggctca
     3421 ggcaaaatga ttaaagaccc ttgcccaact tgtcatggcg caggccgcac caagcaaaac
     3481 aaaacccttt cagttaaaat tccagcaggt gtggatgaag gcgatcgcat tcgtttaacg
     3541 ggtgaaggtg aagcaggcgt aaacggcggg ccaacgggtg atttatacgt ggtggtgcac
     3601 ctgaaagaac acaacgtttt tcaacgtgaa ggcgccaacc tgcattgtga aatgcctatt
     3661 agctttagta ccgcagcatt gggtggcgaa attgaagtgc caaccttaga cggttccgcc
     3721 cgcatgaaag tgccggccga aacacaaact ggcgcagtat ttaggcttag aggcaaaggc
     3781 gttaaaccgc tgcgccaatc tgaacacggc gatttgatgg tgcatgtggt ggtggaaaca
     3841 cccgtgaaat tgactgaaca acaaaaagaa ttgttgcgtc agtttgagac cagcacccaa
     3901 gccgatgccg gcaaacacag cccaaaaaac aaaagctgga tggataaagc tagggagttt
     3961 tttggttaat tcattttata actcgtttca ttaaaaaggc ctgcaatttg caggcctttt
     4021 tgattttgtt ttaaggctgc ataacaaaac atttgcgtcc tacatttgca ttgattatga
     4081 taccgacaag accacctaac cttaagattt aaaaagaaaa atcacatgaa aataacaatt
     4141 ggtaaagtat tattatacgg acgcacaggt ctatatataa ctgttagccg caatgaaaat
     4201 cattagctgg aactgcaatg gtgctttgag aaagaaactt gaaccaatct cggcatttaa
     4261 tgcagatata tacatcattc aagaatgtga agaccgagca aaaatcaatg ataaaaatta
     4321 tcagtcgtgg gctggtcagt atctttggta tggccataat aaaaataagg gtgtgggggt
     4381 atttgcaaaa aatggcttta gcttaacgcc gcttccactt gaatcaggaa ggcttgagta
     4441 ttttctccct tttaaggtca atgaatcttt tacatttcta gctgtatgga ccagagaagc
     4501 aaactcacct acatttaaat acattgggca aatgtggaaa taccttcagc tacataaaaa
     4561 ccaacttaaa agcgataaaa cagtaatcat cggcgattta aatagcaatg tgcgttggga
     4621 tgcatgggat cgttggtgga atcattctga tgttgtgagg gaacttgaag gaattggcat
     4681 ttgtagcttg tatcaccata tttacaaaga agatcaaggc caagaatcaa aaccaacatt
     4741 ttatttgcag cggaatattc aaaaaccata tcatattgat tatgcattta tttctaaaga
     4801 aatggcgcca tcagctagtc tcagcgttgg cgagaaagaa atttggcttg aacatagtga
     4861 ccacatgccg ttatcggttc acatttatgc ctaacctcac gttcagcaag gacaatcaac
     4921 agggccgacg acttcgcctg ttttcggcct tgtcgcctgc cggttaattc aaacgttaaa
     4981 tcttcaccct acccacatca atccaacttt ctcatctaaa ataaccccat gccacacata
     5041 ctgattattg aagatgagcc tgccattgca gaaaccctga tttacgcgtt taaagaggcg
     5101 gggattgcca ccacgcacaa aatgctgggc ggtgaagcca cccctgcgct tgcacaacaa
     5161 gccattgatt ttgtggttct tgatgttggc ctgcccgaca tgacggggtt tgatgtttgc
     5221 aaaaaaattc gtgaaacttc gcaagttcct attttatttc tcactgcgcg taatcatgag
     5281 attgaccgta ttgttggcct agaaattggc gcggatgact atgtgaccaa gccatttagc
     5341 ccgcgtgaag tggtggcgcg ggtaaaggct attttgcggc gcagtgagcc gcaagccaat
     5401 catcataaaa atcacacttt tatgatggat gcacaagcga atcgcattca gtatcgcggg
     5461 cagtatttag agctgacgcg ttatgaattt ttgctgctta aaaccttaat cagccagccg
     5521 cagcgtgtgt attcacgtaa tcaattgatg gatattgtgt gggccaacgc tgaaaacact
     5581 ttagagcgca cggtagatgc gcacattaaa accctgcgcg ccaaactgcg cgaggtggac
     5641 gcggccactg aagcaattgt gacgcatcgc ggtatgggtt atagcttgct ggcataagca
     5701 atgaaaatca gtctgaagat ttttcttgga tattttctat tagtgggctt agccgcgtgg
     5761 tttgtgctca atgtgtttgt agatgaagtc aagcccggcg tgcgccaagc catggaggac
     5821 gcgctggttg ataccgccaa tgtacttgca gaactcgcca ccgatgacat taaaaacggc
     5881 acgattcaaa cgggcaactt tgcccaaagt ttggcgcact atcaagcgcg tagcaggcag
     5941 tctaacccaa ataatgcaaa tatttggggc atcaacaaac aaacggctga ctaccgcgtg
     6001 tatattaccg atgccaaagg aatggtgatt tatgactctg ccaatttggc gctggggcaa
     6061 gattattcgc gctggaacga tgtttacctg actttgcgcg gtaaatatgg cgcgcgttct
     6121 agcctagtag tgcccaatga tgaaaatagc gacacgatta tgtatgtggc cgcgcccatt
     6181 aaagtgggca atcaaattat cggttcatta accgtagcca agcccaatcg cgcgcttttg
     6241 ccatttattg agcgtgctca gcaaaaaatt attcgttggg gtgcgctatt gctggtttta
     6301 tcgctcatca tcggcacgct ttttacttgg cgctttacac gtaaaatcaa tcagttacgt
     6361 gattacgcca tgcgtgtgag ccaaggccaa aaagccacac cgccaacttc aagtaatgat
     6421 gagctgaccg aactggcgct ggccatgcaa accatgcgcg ttgaattaga cggcaaacag
     6481 catgtgcagg attcaattca gcacttaacg cacgaactca aaagccccat gacggccatt
     6541 caaggtgcgc ttgagctgat ttcgcccaat atgccggctg acgcccaagc gcatttttta
     6601 gcgcaaatta aagtgcaatc cacccgcgtg caaaatatta ttcaaaatat gctgggcttg
     6661 gcggcattgg aacatcagca gcaattaacg catcttacgc acatcaacct tgccgattta
     6721 attcgcacac aaatggcaca tttggctgaa aaatccaccg cgcgcaatgt gcagtttcag
     6781 ctcactgaag aacatgcagt cagcctacgc gcagaggctt ttttacttgg gcaagccatt
     6841 tacaatgtgc tggataacgc actggatttt agccccgaaa attcgcacat tcaaattcat
     6901 atcagccagc agcaacatca ggcgcatatt cgcattcaag accaaggcac gggcattccg
     6961 gattacgcac tgactaaaat ttttgataaa ttttactctt tgccccgccc gcaaaacgcg
     7021 cccaatggcg gccaaaaaag taccgggttg ggcttaaact ttgtgcaaga agtgataaaa
     7081 ctgcaccttg gcgaaattca tattgaaaat aacagcgcct cacaaggtgt aacggtttca
     7141 attttattac cgttaaacat ttaatatgtg aacttttgca tgcaaattca gtgcaaataa
     7201 catatcaagt tttagcattc aaaaaaacgt gttttttagc caaatttaac cccaaccttt
     7261 ttcgatgctc tgcgagcctt ataaaaaagg cttgcagagc ataatttttt tctcgtagtt
     7321 aaaatgggta ttttaagcat tgcaatacgc acttaaaaaa gcgttaaatt tgtaactttt
     7381 tttaaaactt cacactcact tcacacaacc gcatcaacac ttcatccagc ctgattattc
     7441 tgcacattcc acattgtttt taataggagt gagtcatgaa tcgggcattt atttttaaag
     7501 tcatcggcat atttttgctg accattgtca tgtcatgggc ggtgatgtat atcaattcgt
     7561 tgattaaaga gcggcaatat cggcaagaag aagttaaact gcaaattgcc agcagttcgg
     7621 ctggcgcgca aaccattatc gggccggtgt tggcgattcc gtatattgaa gaatatgaag
     7681 aaacaattac cgagaataaa atcaagaaag ttgaaaaacg taaggcggaa tatgtggtct
     7741 acgcattgcc ggaagacctg caactcagcg gcggctttac taatgaatac aagaagttag
     7801 gcatttacaa agccttaatg tatcagttgg gcggcaaagt gaaaggcacg tttaatttag
     7861 gcgctgattt taaaatcacg ccgcagcatg ctggcgcaaa aattaccatg ttacctgcct
     7921 atgtgagtat gggcattagc gacacgcgcg gcatcaacgg caagccagcg ctaacgctga
     7981 atcatgaagt ttatgcgttt gcgcagggca caaagttgcc ggtgttgggc aatggtattc
     8041 atgcagaatt aggtaaactg gatgttgtag caagcaaaac gctcaacttt gaatttcaac
     8101 ttaacctgcg cggtatggag aattttaact tcacaccaat agcagatgac aacaaaatcg
     8161 cgttgcaatc cagctggcaa cacccgcatt ttaacggcag ctttttgcct acgcatcaca
     8221 ttaccccggc cggttttgag gcaaaatggg cggtgtcttc gctttcaagc agcaaccaaa
     8281 caaaattatt gaatatgctt gagcaacgtt ggaatgagaa ttctgcagca agcgcggcag
     8341 ccacggcatc acgcgacagt gcctacccag ccgctgccca aaccgcatat cacaacagca
     8401 acttagagtc gcttgccatc ggctttgtag aacccattaa cgtctatagc caatcagacc
     8461 gcgccacaaa atatggttta ttattcatcg gcttaacatt tgctggcttc tttatttatg
     8521 aaattttaaa gcaattgcgc attcaccctg cgcaatacac gctggtgggc ttggcaatgg
     8581 cgctgtttta cttgctgctg acttcacttt cagaacacat cagctttgcg ctttcatacc
     8641 tcacagccag cttggcgtgc gtggcgctac ttggctatta cctcacgcat gtgcttaaaa
     8701 gcaaaagcca cggcctactg tttgcaggat tactcacggc tttatatggc gccttatatg
     8761 gcatattggc ttcagaagat aacgctttat tgatgggcgc cttgctggtg tttggcttgt
     8821 tggcagtgac gatggtaatc accagaaaag tggattggta tcagattagc agcaaagaaa
     8881 acatgcaaat caacgcgtaa aacaacacaa cccttttgac ctcctctgca agcctttgtt
     8941 tataaggctt gcagaggcat aaaaaatcct cgtacaaata atgcgcttcg tttagaggcg
     9001 cctacttttg gtggggcaca tattctacgc gcttaatctt gacggttacg cgaggaaaat
     9061 taggtctgat ttagatagtt ttgaatggtt tttagctcag cgttggcatc gccaaaatct
     9121 tcataatgtt tgccagccgt gcgcgcatac caatacttgc tgttccacgc atcgccttca
     9181 attttgtgca gtaccgcatg aatccaattg gctgtggcgt ctgaataatc ttgcgcaatt
     9241 ttgtgcgact tgtcccaatc gccatctagg gcagcttgca cggcaagtaa taatgcggtt
     9301 ttattcatgc ttcaggtaat acttcaaaaa aggcgagttt tttgtgtcta ggtaattttt
     9361 ttagttgcgc ttcagccaca ctggcgcttg agcggcccgc ataaggtttt gtggcaagca
     9421 cttttagggg tggattagcg cgtgtatagc gcgcaccttt tccacttaaa tgcgccgtaa
     9481 aacgtgcttc tacatcattg gtaatgcctg cataataggc accgtttttg cattctagca
     9541 agtataaaaa ccagcaatct tgcacaacgt ctatcaacat ctatactccc cgttcgcccc
     9601 gaccttgtag aagggctgat tcaatgaacc atgcttcgac aagctcagca cgaacggcgg
     9661 ttgttaagcg tttagtaggt tccagcctga atgaattcaa ctttatagcc atccgggtct
     9721 tccacaaac
//
LOCUS       DAOQSI010000067         9697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_6392_length_9697_cov_6.071873, whole genome shotgun sequence.
ACCESSION   DAOQSI010000067 DAOQSI010000000
VERSION     DAOQSI010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 9697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9697
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6392_length_9697_cov_6.071873"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PKL42_05670"
     CDS             <1..187
                     /locus_tag="PKL42_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNU66599.1"
                     /translation="NDVLVVKADVVQPELAKSASDKVVEVKNEKPQERLLPFIASVVL
                     EVDLKAQTMLVDWDEDF"
     gene            203..940
                     /gene="trmD"
                     /locus_tag="PKL42_05675"
     CDS             203..940
                     /gene="trmD"
                     /locus_tag="PKL42_05675"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNU66600.1"
                     /translation="MAYQFDVVTLFPEMFEAITQHGITSRALQHNIYHVQFWNPRDYT
                     TDNHKTVDDRPYGGGPGMVMLVEPLEKAIAAAKTAQQEKHIESWVVHLSPAGKPLTHQ
                     KVMQLSKKPGLVLLASRYEGVDQRLIDAEVDEEISIGDYVLSGGELPAMALMDAIIRQ
                     LPGSLGDGDSAIEDSFVDGLLDCPHYTRPEEYKGVKVPEVLLSGNHAKIKQWRLKAAL
                     QRTRDLRPDLLAVRPLTKEETRLLQHD"
     gene            1005..1400
                     /gene="rplS"
                     /locus_tag="PKL42_05680"
     CDS             1005..1400
                     /gene="rplS"
                     /locus_tag="PKL42_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNU66601.1"
                     /translation="MADIIKMLEEEEIARLGKTVPDFAPGDTVVVGVNVVEGTRKRVQ
                     SYEGVVIAKRNRGLNSSFIVRKISSGEGVERTFQTYSPLIASIELKRRGDVRRAKLYY
                     LRERSGKSARIKEKLVARDREVLAPVEKA"
     gene            1498..2001
                     /locus_tag="PKL42_05685"
     CDS             1498..2001
                     /locus_tag="PKL42_05685"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNU66602.1"
                     /translation="MKSQHKHIQVYEAVVETPFGAVGIVSQGMQVVIDLLPERLEQKP
                     TTQKLAQNIAAQIEAYLKNANSGFNLPIKLKGTPFQRNVWQIIDAIPSGQVLTYSQIA
                     EQLKSSPRAVANACGANFLPIVIPCHRVVAKDGLGGFMRDAEHGLTIKKWLLKHEGAI
                     DENGKLV"
     gene            2006..2905
                     /gene="xerD"
                     /locus_tag="PKL42_05690"
     CDS             2006..2905
                     /gene="xerD"
                     /locus_tag="PKL42_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNU66603.1"
                     /translation="MALESAEAQQLDSFIDHLWLEDGLSKNTLESYRLDLTSFTLWLP
                     TQKKQLLTVEEADIQQYLAVKFPQSKPRSISRLIATLRRFYRYCLRENLIKTDPTIQI
                     QSPKLPRSLPKSLNEEEVIALLNAPNLNAPAGIRDKAMLELLYACGLRVSELVNIKAT
                     EVSINDAVVRVTGKGSKTRLVPMGEDAADWISRYLREARPALLHKRLCDALFVTNRGE
                     AMTRQAFWYLIKRYALLAGITKPMSPHVLRHAFATHLLNHGADLRVVQMLLGHADIST
                     TQIYTHVARERLKQLHSVHHPRG"
     gene            complement(2961..3314)
                     /gene="folB"
                     /locus_tag="PKL42_05695"
     CDS             complement(2961..3314)
                     /gene="folB"
                     /locus_tag="PKL42_05695"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNU66604.1"
                     /translation="MDTIFISELKVQTKLGVPEWERMTAQTIILDIEIGYDLTKACRS
                     DVIANTIDYGAVVGRIRETLTDNSFQLVEALAEHICQLILQEFGALNVKVKVAKPAIL
                     AGLKSLGVIVERAKT"
     gene            3382..4002
                     /gene="plsY"
                     /locus_tag="PKL42_05700"
     CDS             3382..4002
                     /gene="plsY"
                     /locus_tag="PKL42_05700"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNU66605.1"
                     /translation="MNELGFIPVTLVPAVWIVAAYLLGSVAFGILVSKAFGLPDPRTV
                     GSGNIGATNVARSGKKSAAVLTLLGDVLKGWLPVWLALQSDMLMWVVAWVGLAVFFGH
                     LYPIYYKFKGGKGVATALGVMLALSPWVALAALATWLVVFAISRYSSLAAISAAALAP
                     VYAWFFLSAYKDYFMTVLVMALFLIWRHRSNIQKLLAGTESGFGKK"
     gene            complement(4142..5194)
                     /gene="tsaD"
                     /locus_tag="PKL42_05705"
     CDS             complement(4142..5194)
                     /gene="tsaD"
                     /locus_tag="PKL42_05705"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNU66606.1"
                     /translation="MLILGVESSCDETGLALYDTERGLLAHALHSQVEMHAEYGGVVP
                     ELASRDHIRRVLPLTERVLKDADKKLSDIDAIAYTQGPGLSGALLVGTSFAESLAFSL
                     QIPTICVHHLEGHLLAPLLEDNPPAFPFVALLVSGGHSQLMRVDGIGQYELLGDTLDD
                     ASGEAFDKTAKLLGLGYPGGPALSKLAELSHSTHGKPRFKLPRPLLNSGDLNFSFSGL
                     KTAVLTLVNTHTDLDLQTKADIAWEFQEAVTEVLTTKCMAALRETGLDNLIVSGGVGA
                     NSKLREKLNAATKRKLCKVSYPRLEFCTDNGAMIAFAGAMRFSAMRLQAMQNINKNDY
                     SFSVKPRWNLGELKAP"
     gene            5387..5599
                     /gene="rpsU"
                     /locus_tag="PKL42_05710"
     CDS             5387..5599
                     /gene="rpsU"
                     /locus_tag="PKL42_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNU66607.1"
                     /translation="MSSVRVKENEPFDVALRRFKRLIEKTGLLNDLRAREFYEKPTWE
                     RKRKAAAAVKRQYRRLMKQTLPAKLY"
     gene            5609..5917
                     /locus_tag="PKL42_05715"
     CDS             5609..5917
                     /locus_tag="PKL42_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3144 domain-containing protein"
                     /protein_id="HNU66608.1"
                     /translation="MADSKEVEAKPEDVFWQLTDNFIGHANSLVPAHDVGVVNASMIF
                     ASARFNTFVIASQCGTKEAFEAEKAAALEYFVNQYKIALTEHMAEVQTNFDKYISPSA
                     "
     gene            5923..6366
                     /locus_tag="PKL42_05720"
     CDS             5923..6366
                     /locus_tag="PKL42_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNU66609.1"
                     /translation="MSLKQQISEDMKNAMRAKDSARLGAIRLLQAAIKQREVDERIEM
                     TDADVIAAIEKMLKQRRDSIAAFELANRTDLADIEKFEVSVLQTYMPQQLSDEAIHQI
                     INQVIADTGAQGAKDMGKVVGLVKPLVAGVADMGKVSGLIKARLS"
     gene            6451..8220
                     /gene="dnaG"
                     /locus_tag="PKL42_05725"
     CDS             6451..8220
                     /gene="dnaG"
                     /locus_tag="PKL42_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNU66610.1"
                     /translation="MIPESFIQELLNRVDIVDVVDKSVPLKKAGANYSACCPFHNEKS
                     PSFTVSPTKQFYHCFGCGAHGTALGFLMEYQGLSFVEAVHELAKMVGLQVPQVQATSE
                     AREQAHAQQKVAASLQEVLQQAADYYKAELKKSPRAIEYLKARGLSGKVAAKFQIGYA
                     PAGWQNLQSVFPQYENEALNVAGLVVENEQGKRYDRFRDRIMFPIHDQKGQVIGFGGR
                     VINPEDTPKYYNSPETPLFQKGHELYGLYLARRAIRDAGRALVVEGYMDVVALAQYGI
                     EYAVAALGTATTPVHIAKLMRQADEIVFCFDGDNAGRTAAWRAAMNALPALSDGLKLS
                     FLFLPKEHDPDSYVREFGKEKFEAEMKVAMPLSQYILKHLSENNTLQSQEDKVRFLNE
                     AEPILQQIKAPRSAMFLRKKVAELAQLSTDEMHSMLKLPSLNKAPVQARPKFSRTPSS
                     LHKRFCLMLLMQPSFAAAEDLLWVVGQGDETQLLRHTIETCLIHPHSKPVVIMRALET
                     KVDAKLLRDLERDLHLLDETLDMALELTGARHQLKQTYTQQSSNVLLAHLSEKPLSDL
                     TEDEKAMLRTVGFKTLATKLEPK"
     gene            8310..>9697
                     /locus_tag="PKL42_05730"
     CDS             8310..>9697
                     /locus_tag="PKL42_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNU66611.1"
                     /translation="MARPKKSEREAAKEKNEFMTPAIQEANAEERRVRLKSLIVLGKE
                     RGYLTYAEINDHLPDDVQGSEQIDGIIGMINDMGIQVYEEVPDAEVLLMSDAPAAVTD
                     EDAVEEAEQALATVDSDFGRTTDPVRMYMREMGTVDLLTRESEIEIARRIEDGLKHMV
                     QAIAACPTTIAALLEMVDKVENDEMSVDDLVDGLIDADVGLDDAMTVEAAEDEEPELV
                     DDDEDDGDEDGAKAAAISAEALARLKEEVVNRFAVIRAANQKMQLILAESGSQDSEYQ
                     RLLAEILEELTAFRFSAKQVEGLCDQVRAIIEAVRGHERKVMDFCVEKSGMPRAHFIK
                     SFPGNECDLSWLEQELKAEKPYIEKLARYNHSIIDQQQRLIDLENKVGIPIRELKEIN
                     KQMSTGEARARRAKREMIEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFE
                     YRRGFKFSTYAT"
BASE COUNT         2800 a         1923 c         2381 g         2593 t
ORIGIN      
        1 aaatgatgtg cttgttgtga aggccgatgt tgttcaaccg gaattggcaa agtcagcttc
       61 ggataaagtt gtagaagtta aaaatgaaaa gccgcaggaa agattgttgc cttttattgc
      121 atcagtggtt ttagaagtgg acttgaaagc tcaaacgatg ttggtcgact gggacgaaga
      181 cttttagttt agtacagcaa ccatggctta tcagtttgat gtagtaacgt tatttcctga
      241 aatgtttgag gccattaccc agcatggcat taccagcagg gcgttgcaac ataatattta
      301 tcacgtgcag ttttggaatc cacgtgatta cactactgat aatcataaaa cggttgatga
      361 caggccgtat ggcggtggcc ctggcatggt gatgctggtt gagccgctcg aaaaggcaat
      421 agcggcagca aagacggcgc aacaagaaaa gcatatagaa agttgggttg tccatctttc
      481 accagcggga aagccgctga cgcatcaaaa agtgatgcag ttaagtaaaa agccaggctt
      541 agtattactg gctagccgtt atgaaggggt tgaccaacgc ctgattgatg ctgaagtaga
      601 tgaagaaata tccattggtg attatgttct aagtggcgga gagttacctg ccatggcatt
      661 aatggatgcg attattcgcc aattgccagg aagtttaggg gatggggatt cagccattga
      721 agattcattt gtcgatggat tgttggattg cccacactac actaggcctg aagaatataa
      781 aggtgtgaaa gtgcctgaag tgttgttatc aggcaatcat gcaaagatta agcagtggcg
      841 tttaaaagcg gcattgcaaa gaactcggga tttacgtccc gatttattgg ccgtaaggcc
      901 gttgacgaaa gaagaaacac gactgctaca gcatgattag agagcaggaa caagttactt
      961 cataattaaa aggaaccccg caagggagaa aaaaggtaaa aaagatggca gatatcatta
     1021 aaatgttaga agaggaagaa attgctcgat taggcaaaac agttcctgat tttgcgccag
     1081 gcgacacagt cgtggttggt gtaaacgttg ttgagggtac tcgtaaacgt gtacagtcat
     1141 atgaaggtgt tgtgattgct aaacgcaacc gtggcttaaa ctcatcattc atcgtgcgta
     1201 aaatttcttc aggtgaaggt gttgagcgta cattccaaac atattcacca ttaattgcga
     1261 gcattgagtt gaaacgtcgc ggtgatgtgc gtcgcgctaa actttactac ttgcgtgagc
     1321 gttcaggtaa atctgcgcgt attaaagaga aattagttgc acgtgacaga gaagtattgg
     1381 cgccagtaga aaaagcataa caaacgcttt aacgcagtca caaaaaggcc ctaaaactgt
     1441 acgcggttta gggctttttt atttgcacta acatcaataa tagcgtttaa ttactgtatg
     1501 aaaagccagc ataaacacat tcaagtatac gaggcggttg ttgaaacgcc atttggcgcg
     1561 gtggggattg tgtctcaagg aatgcaagtg gtcattgatt tattgccaga aaggcttgaa
     1621 caaaaaccca ccacgcaaaa attagcacaa aacattgccg cacaaattga agcgtattta
     1681 aaaaacgcca acagtggatt taacttgccc atcaaactga agggtacgcc atttcagcgc
     1741 aatgtttggc agataataga tgcaattcca agtgggcaag tgctcactta tagccaaatt
     1801 gccgaacagc ttaaatcaag cccgcgcgct gtggccaatg catgcggcgc aaattttctg
     1861 cctatcgtca tcccgtgcca tcgcgtggtg gcaaaagatg gtttgggcgg ttttatgcga
     1921 gatgctgaac atggattaac tataaaaaaa tggcttctca agcatgaagg tgccattgat
     1981 gaaaatggca aattagtttg aatcaatggc gttagaaagt gcagaagccc agcagttaga
     2041 tagctttatt gaccaccttt ggttagaaga tggcctatcc aaaaacacgc ttgaaagcta
     2101 tcgcctcgat ttaaccagtt tcacactttg gttgcccaca caaaaaaaac agttactcac
     2161 agttgaagaa gcagatattc aacaatatct cgcggttaaa tttccacaaa gtaagccacg
     2221 cagtatcagc cgattgatag ccactttgcg gcgtttttat cgttattgtt tgcgtgaaaa
     2281 tcttattaaa acagacccga ccattcaaat tcagtcgcct aagttgcctc gtagtttgcc
     2341 caaaagcctg aatgaagaag aagtcatcgc gctactcaac gcgcctaatt taaatgcgcc
     2401 agcaggcata cgcgataaag ctatgctgga gctactgtac gcctgcggtt tgcgggtatc
     2461 agagctggtc aacattaaag cgaccgaagt gagcatcaat gatgctgttg taagggtgac
     2521 gggaaaaggc agtaaaacga gattagtgcc aatgggtgaa gatgcagccg attggatttc
     2581 acgttatttg cgtgaggcaa ggccagcgtt gctgcataaa cgtctgtgcg atgcgctgtt
     2641 tgtaaccaat cgaggcgagg cgatgacgcg tcaagctttt tggtatttga ttaaacgcta
     2701 tgccttgctg gctggcatta ccaagcctat gtcgccacac gtattgcgcc atgcgtttgc
     2761 cacgcatttg ctgaatcatg gcgccgattt acgtgtagta caaatgttgt tagggcatgc
     2821 ggatatttca accacgcaga tttatacgca tgtggcgcgg gagcgattga agcagttgca
     2881 tagtgtgcat cacccacgag gataggtgtg gttgcaccat ttaaattgtt aacgccttaa
     2941 cggctaaatg caaaatcgag ttatgttttt gctcgctcaa cgatcactcc caacgatttt
     3001 aacccggcca aaatcgcagg cttagcgact tttaccttca cgtttaacgc accaaattct
     3061 tgtaaaatca attggcaaat atgttcggcg agggcttcta ctagctgaaa gctgttatca
     3121 gttaaggttt cgcgtatgcg acccaccaca gcgccgtagt cgatggtgtt ggcaatgacg
     3181 tcgcttctgc aggccttagt taaatcgtag ccaatctcaa tgtctaaaat aatggtttgc
     3241 gcggtcatgc gttcccattc tggcacgccc agtttggtct gtactttgag ttcgctaata
     3301 aaaatggtgt ccatacaatg tttgtttatc gtataaaata taatgctgtg attttagagc
     3361 atctcaagaa ggttgggcaa aatgaatgag ttaggtttta ttccggtgac gttagtccct
     3421 gctgtgtgga ttgttgcggc gtatttgctg ggttcggtag cttttggtat tttggtgagc
     3481 aaggcatttg gtctgccaga tccgcgcacc gtaggcagcg gcaatattgg cgccacaaat
     3541 gtagcgcgta gcggtaaaaa atctgccgct gtgttaacgc tgctgggtga tgtactcaaa
     3601 ggctggctac cggtatggtt ggcgttacag tcagacatgt taatgtgggt agtggcttgg
     3661 gttgggttgg ctgtgttttt tggtcatttg tacccgattt actataaatt taaaggtggc
     3721 aaaggcgtgg cgaccgcact gggcgtgatg ttggcgcttt ccccatgggt tgcgttggcg
     3781 gcgctagcaa cttggctagt ggtgtttgcc atttcacgct actcatctct tgctgcaatt
     3841 tccgctgccg cactggcgcc cgtgtatgca tggtttttct tgtctgcgta taaagactat
     3901 tttatgaccg tgctggtgat ggcgctattt ttgatttggc gtcaccgcag caatattcaa
     3961 aagttgctgg caggcactga atctggtttt ggaaaaaagt agcgcttttg caacatcaaa
     4021 ttggcattaa aaaatacgct taattcacct aaatttaaca ccaacctttt ttgatgccct
     4081 gcgagcctta taaataaagg ctcgcagggc ataaattttt tctcggacga aagcgaggcc
     4141 attaaggtgc ttttaattcg cctaaattcc atcttggttt tacgctaaaa ctgtagtcat
     4201 ttttgtttat attctgcatg gcttgcaacc gcattgcact gaatctcatt gccccggcaa
     4261 aagcaatcat cgcgccgtta tctgtgcaaa actctaatcg cggataactc actttgcaaa
     4321 gtttgcgctt agttgcggcg ttgagttttt cacgtagttt gctattggcg cctacgccgc
     4381 cagatacaat caagttatct aagcccgttt cgcgtaatgc cgccatacat tttgtggtta
     4441 agacttcggt gacggcttct tgaaactccc aagcaatatc cgctttggtt tgcaaatcaa
     4501 ggtctgtgtg agtgtttact agcgttaaaa cggctgtttt taaaccacta aagctaaaat
     4561 tcaaatcacc gctattgagc agtgggcgcg gtaatttaaa gcgcggtttg ccatgggttg
     4621 aatgagaaag ctccgctagt ttggaaagcg ctggtccgcc tggataaccg agccccaaca
     4681 atttagctgt tttatcaaac gcctcacctg aggcatcatc taaggtatcg ccaagcaatt
     4741 cgtattggcc aataccatca acgcgcatca actgactatg cccgccagaa accagcagcg
     4801 ccacaaacgg aaaagctggt ggattatctt caagcaaagg ggctaataaa tggccttcta
     4861 aatgatgcac gcagatggtc ggtatttgca ggctaaacgc caaggattcg gcaaagctgg
     4921 tgccaaccag caatgcgcca gatagccctg gtccttgtgt ataggcgatt gcatcaatgt
     4981 cacttagttt cttgtcggca tcttttaaaa cgcgctcggt gagcggcaat acgcgccgaa
     5041 tatggtcacg cgaggcgagt tctggtacca cgccaccgta ctcggcgtgc atttcaacct
     5101 gtgaatgcaa ggcgtgcgcc agtaaaccgc gttcggtgtc gtataatgca agccctgttt
     5161 cgtcacagga agattcaacg cctaaaatta acatgaatgg tgatggatga actgtaaagg
     5221 ttgaattatt gcgcatttaa ctgattaatg cataaaagta attgaataaa aacaattatg
     5281 cgattaaaat agtcggcttt tctcagttta taaaccttgc atgtgcaaaa tggttaagaa
     5341 cttgagcata atttaaacta acttcttacc aaataagaga gattgaatgt ctagtgtacg
     5401 cgtaaaagaa aatgagccgt ttgacgttgc ccttcgccgt tttaaacgct taatcgaaaa
     5461 aactggttta ttgaatgatc ttcgcgcacg cgaattttat gaaaaaccaa cttgggaacg
     5521 taaacgtaaa gctgcagccg cagttaagcg tcaatatcgt cgcctgatga agcaaacatt
     5581 gccagctaaa ctttactaag ttagtctgat ggctgattcc aaggaagttg aagcaaagcc
     5641 tgaagatgta ttttggcagt tgactgataa ttttattggc catgccaatt cgctagtgcc
     5701 ggcgcacgac gttggtgttg tgaatgcgag catgatattt gcatcggctc gctttaatac
     5761 ttttgtcatc gcttcgcagt gtggcactaa agaagcattt gaggccgaaa aagccgctgc
     5821 tttagaatat tttgtaaatc aatacaaaat tgcgcttaca gaacacatgg cagaagtgca
     5881 aacaaatttc gacaaataca ttagtccaag cgcctaaaat ccatgagtct caagcaacaa
     5941 atctccgaag acatgaaaaa cgcgatgcgc gctaaagata gcgcgcgttt aggcgcaatt
     6001 cgtttgctgc aagccgccat taagcagcgc gaagtggacg agcgcataga gatgaccgat
     6061 gctgatgtga ttgctgcgat tgagaaaatg cttaaacaac gacgcgattc aatcgccgcg
     6121 tttgaattag ccaaccgcac agatttagcc gatattgaaa agtttgaagt gagcgtgttg
     6181 caaacttaca tgccgcaaca attgagcgat gaagccattc accaaattat taaccaagtg
     6241 attgccgata ccggcgcgca aggtgctaaa gatatgggta aagtggttgg gttggtcaaa
     6301 ccgctagtcg caggtgttgc agatatgggt aaagtgtcgg gcttaataaa ggcgcgacta
     6361 agttaaatta ggataatccg caatccagcg attaaaaaaa caagggagcg acatcgctcc
     6421 tttttttatt gatgctttat actcacgcat atgattccag aaagttttat tcaagaatta
     6481 ctgaatcgcg ttgatattgt cgatgtggtt gataaatctg tcccgcttaa aaaggctggt
     6541 gcaaattatt ctgcttgctg cccgtttcat aatgaaaaat cccccagttt taccgtgagt
     6601 ccaaccaagc agttttatca ctgttttggt tgcggtgcgc atggtacggc gctgggtttt
     6661 ttaatggaat atcaaggtct tagctttgta gaagctgttc atgaactcgc taaaatggtg
     6721 ggcctgcaag tgccacaagt gcaagcaaca tcagaagcgc gagagcaagc gcatgcgcaa
     6781 caaaaagtgg cggcaagttt gcaagaggtg ttgcagcaag cggctgatta ttacaaggct
     6841 gagcttaaaa aatcaccgcg tgcgattgaa tatttaaaag cgcgcggctt aagtggtaaa
     6901 gttgctgcaa aattccagat tggttatgcg cccgctggct ggcagaattt gcaaagtgtg
     6961 tttccacaat atgaaaacga agcgctcaat gttgcagggt tggtcgttga aaatgaacaa
     7021 ggtaaacgtt acgaccgctt tcgtgaccgt attatgtttc caattcatga ccaaaaaggc
     7081 caagtgattg gttttggtgg gcgcgtgatt aacccagaag atacgcctaa atattacaac
     7141 tcccctgaaa cgccgttatt ccaaaagggc catgagcttt acggcttgta tttagcgcgt
     7201 cgtgcaattc gtgatgctgg tcgtgcgctg gtggtagaag gttatatgga tgtggtggcg
     7261 cttgcacaat acggcattga atatgctgtt gcggcgctag gtaccgcaac cacacctgtt
     7321 catattgcta agctcatgcg tcaagcagat gaaatcgtat tttgctttga tggtgataac
     7381 gctggtcgca cggccgcttg gcgtgcggca atgaatgcgt tgccagcact gagtgatggc
     7441 ttaaagttat ctttcttatt tttgccaaaa gaacacgacc ctgattcata cgtgcgtgaa
     7501 tttggaaaag aaaagtttga ggctgaaatg aaggtggcta tgccgctttc gcaatatatt
     7561 cttaagcatt taagtgaaaa taataccttg caaagccaag aggataaagt acggtttttg
     7621 aatgaagcag aaccaatttt gcagcaaatt aaggcaccac gcagtgcaat gtttttgcgt
     7681 aaaaaagtgg cggaacttgc gcaactttcg acagatgaaa tgcatagtat gcttaaactg
     7741 cccagcttga acaaagcacc agtgcaagca aggccaaagt tttcacgcac gcctagttca
     7801 ttacataagc gcttctgcct catgctgcta atgcagccca gttttgcagc cgcagaagat
     7861 ttattgtggg tggttggtca aggtgatgag acgcaattat tgcgccatac tattgaaact
     7921 tgtctaattc accctcattc taagcctgtg gtcattatgc gagcgcttga aacgaaagtg
     7981 gacgcaaagt tattgcgcga tttagagcgg gacttgcatt tactagatga aacattagac
     8041 atggcacttg agctcactgg cgcaagacac cagctaaaac aaacttacac tcaacagagt
     8101 agcaatgttt tgttggcgca tttgagtgaa aagcccttaa gcgacctcac ggaagatgag
     8161 aaagcaatgt taaggacggt tgggtttaaa actttagcta ctaaactcga gccaaaatag
     8221 ggtttaattc tgttataatt gaaggttaca aaaaatcatt tgccagatac ttttttagaa
     8281 atctggcgtg caaaatttag aggtaagtca tggcgagacc aaagaaaagc gaacgagaag
     8341 cagcaaaaga aaaaaatgaa tttatgacac ctgcgattca agaagcaaat gcggaagagc
     8401 gtcgtgttcg tttaaaatcg ctgatagtac taggcaaaga acgcggctat ttgacctacg
     8461 ctgagatcaa tgaccatctg cccgatgacg tgcaaggctc tgagcagatt gatggcatta
     8521 tcggcatgat taatgacatg ggtattcagg tttatgaaga agtccctgat gccgaagtgt
     8581 tattaatgtc agatgcccca gcagccgtta ctgatgaaga tgccgttgaa gaagctgagc
     8641 aggcgctggc aacggtagat tccgattttg gtcgcactac tgaccccgtg cgcatgtaca
     8701 tgcgtgaaat gggtacggtt gacctgctca cacgtgagag cgaaatcgaa attgcgcgtc
     8761 gtattgaaga tggcctcaag catatggtgc aagcgattgc cgcctgtcca actacgattg
     8821 ctgcgctttt agaaatggtt gataaagttg aaaacgatga aatgagcgtt gacgacttag
     8881 tcgatggctt aattgatgcc gatgttggcc tagatgatgc catgacggta gaggccgcag
     8941 aagatgaaga accagagttg gttgatgatg acgaagatga cggtgatgaa gatggtgcaa
     9001 aagcggcggc catttctgct gaagctttag caagattaaa agaagaagtt gtgaatcgtt
     9061 ttgcggttat tcgggcagca aatcaaaaaa tgcagttaat tttagctgag agcggctcgc
     9121 aagattcaga atatcaaaga ttgttagcag aaattttaga agaactgaca gcgtttaggt
     9181 tctcagcaaa acaagtagaa ggcttgtgcg accaagtgcg tgcaattatt gaagcagtgc
     9241 gtggccatga gcgtaaagtg atggatttct gtgttgaaaa atcgggtatg cctagagcgc
     9301 attttattaa atcattccca ggaaatgagt gtgatttaag ctggctggaa caagagttaa
     9361 aagctgaaaa accctacata gagaaattag caaggtataa ccactcgatt attgaccaac
     9421 agcaacgact gattgatttg gaaaataaag tcggcattcc aatcagagag ttaaaagaaa
     9481 tcaataagca gatgtccact ggcgaggcgc gtgcaagacg tgctaaacgc gaaatgattg
     9541 aagcaaactt gcgcttagtg atttcaattg caaaaaaata cactaacaga ggcttgcaat
     9601 ttctagattt aatccaagaa ggtaatattg gtttaatgaa agcggttgat aaatttgaat
     9661 accgacgcgg ctttaaattt tcaacttatg ctacttg
//
LOCUS       DAOQSI010000068         9660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_6432_length_9660_cov_4.656845, whole genome shotgun sequence.
ACCESSION   DAOQSI010000068 DAOQSI010000000
VERSION     DAOQSI010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 9660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9660
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6432_length_9660_cov_4.656845"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PKL42_05735"
     CDS             <1..331
                     /locus_tag="PKL42_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNU66612.1"
                     /translation="WSPKALRGGQGAQFVLPIVERADLVTVLNHFQGNSYATTMQGES
                     LYQQNLSAPTAFVIGNEGAGLKHKTIAAATKSMSVPMQANSVESLNAAAATAICLFER
                     VRQLAIK"
     gene            340..828
                     /locus_tag="PKL42_05740"
     CDS             340..828
                     /locus_tag="PKL42_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HNU66613.1"
                     /translation="MNRKCKWSAGFSAIEMMVVVAIIGILAMIAIPNSMGRIVKEQVK
                     AALPLTDVAKEPIAAAWKALKLLPKDNEEAGLPVAEKIVSNFASAVTVEEGAIHLTFG
                     NKAHKVLQGKVITMRPAVIEESSIVPVEWVCGNAKPPEKMVVKGANKTNVAEEFLPML
                     CR"
     gene            complement(858..1733)
                     /gene="hslO"
                     /locus_tag="PKL42_05745"
     CDS             complement(858..1733)
                     /gene="hslO"
                     /locus_tag="PKL42_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HNU66614.1"
                     /translation="MHNQDLLSRFIFDNTAVRGNHIQLSQTIQQALQHHNYPTLLKTA
                     LSELMVASALLAATLKMQGALVLQIQGRGKLKLLVVECTSQLEIRATAKWTGDIVEDN
                     FIALLEQGQFVITLDPKDGRQTYQGIVPIEGHTIAEILQNYMQRSEQIETRIWLTSAH
                     NQAAGMLLQKLPEQLEIDTDAWNRICQLADTTTKQELIELDANELLRRLFHEENVRIF
                     EPHTAQFKCSCTRKNVGNMLRMLGTEEIASILAERESIEVHCDFCNQAYVFDAVDAAQ
                     LLASETTLDTPKLAH"
     gene            1850..2053
                     /locus_tag="PKL42_05750"
     CDS             1850..2053
                     /locus_tag="PKL42_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66615.1"
                     /translation="MANQIISQTQLAGDASCKVLYNKVNDKVELEIGGTSIRLEANQF
                     FVMHEMLRKAVARLVMQTDLTKV"
     gene            2150..4312
                     /locus_tag="PKL42_05755"
     CDS             2150..4312
                     /locus_tag="PKL42_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNU66616.1"
                     /translation="MQHYKSNTTQFAGAKLKTISACVMVLYATHASLALAEPASEDNA
                     LDMQSIRIIGSQSQLLNIPGSALLIEQQDLEESHVMTTNEALRKAPGVVVRDEEGIGM
                     RPNIGMRGLNPTRSTKVLLLEDGIPVTYAPYGANESYYHPTVDRFSSIEVLKGASQIK
                     YGPLTAGGVINYITPPAPEKFGGHLSVTAGNRDFLNTKMNVGGNGLLFDYTHKEGDGA
                     RDNTHSNVEDLNIKFNQALNDQHSITLRANWFSEDSQVSYSGLTQKEYENFGGTYNPF
                     NHDNFATTRYGVSATHDWQLNDSALLTTNFYYSYFDRDWWRQQSNTTASNVATGTGCA
                     AFSADRIAGLATNGDNCQGVQGRLRTYNTYGVEPRLTITHALGELEMGAKAHFEDQER
                     RQVNGTNATARTGTLVENNERKTDAYSGFISNRFTIGQFSITPAIRYEHIENERTNRL
                     TGSHGSANLQEWIPGIGVAYNPNQNITLFAGVHEGFAPPRTEDLITNSGGSVEVDAEK
                     STNYELGFRAKPVKGLQVDATAFYNDFSNLIAVGSVASNVSLSQGEATFAGLEFSGQY
                     DFDNGFYSRLAYTWLPVAEQDTPFKQVSNGAAVAGSSKGNRQPYAPKNTLTAAVGYRV
                     ASWNAQLEAVHVGKQYSDFAETNEVPNTALGATGQFGEIASYTIYNAAVNYKFEPYKT
                     TVFVTGKNLFDKDYITDRTRGILTGMPRLVQVGVRYDF"
     gene            complement(4495..5388)
                     /gene="gluQRS"
                     /locus_tag="PKL42_05760"
     CDS             complement(4495..5388)
                     /gene="gluQRS"
                     /locus_tag="PKL42_05760"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HNU66617.1"
                     /translation="MYVGRFAPSPTGPLHFGSLVAAVASYCDAKVNHGKWLLRIEDLD
                     KPREIAGAADTILRQLEAFGFEWDGEIIYQSQRHAFYTEAFQQLNAQHLLYPCTCTRK
                     EITDSSQQWGIEGVIYPKTCLTHALKPLAPSAWRIKTNATPIQFEDAIQGTLSQQLNE
                     AVGDFILKRADGLFAYQLAVVVDDAAQDITHIVRGADLLASTPRQIYLQQLLQLNTPH
                     YAHVALACNAAGEKLSKQTLAKSIDASQANQRLFEALSFLGQHPPDELKNATMADLWH
                     WAMTYWQLKNVPKIKNMVVKM"
     gene            complement(5395..5706)
                     /locus_tag="PKL42_05765"
     CDS             complement(5395..5706)
                     /locus_tag="PKL42_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3301 domain-containing protein"
                     /protein_id="HNU66618.1"
                     /translation="MELVLIFILFAVVWFWQDSLAKRERATLLGRELAARFQLQLLDE
                     SVACSNLWLGRNSRGQAQLQRTYEFDVSANGADRLPCHLILLGNQLSSWHIPPYLQPD
                     H"
     gene            complement(5752..6930)
                     /locus_tag="PKL42_05770"
     CDS             complement(5752..6930)
                     /locus_tag="PKL42_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNU66619.1"
                     /translation="MTNEQALNYMHNLLRGMLDKKASDLFISADFPPAMKIDGKMTPV
                     TQQKLTGDLTKAFADALMNEKQRAEFAVDKECNFAIYPAGIGRFRVNVFVQQEKVGMV
                     LRTITTQIPNFDDLGLPEVLKDVMMTKRGLVILVGGTGSGKSTTLAALIDYRNQNSYG
                     HIITVEDPVEYVHTSKNCLITHREVGRDTNGWFNALKNTLRQAPDVILIGEIRDRETM
                     EFALAFAETGHLCMATLHANSSNQALDRIINFFPEERHAQLHMDLSLNMKAFVSQRLV
                     SRTGGGRCAAIEILLNSPLVADLILKGETNMVKEAMAKSVELGMQTFDQALFNLCEEG
                     RITQEDALRNADSINELRLRFKLHSKNAGEKSSAFEKPSLSLHVDEPEDPDEDPMAKF
                     "
     gene            7137..7811
                     /locus_tag="PKL42_05775"
     CDS             7137..7811
                     /locus_tag="PKL42_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66620.1"
                     /translation="MSQMREKEAIDAYFKLVQAKGADNATLKKRVLFLEQLSEHLAEK
                     NVTGQDYREAVEALMETIPPEDWHPSLTTAREYFPFWMHDIKAIAAHNRFPGFDIPPN
                     QWRPATTTLKTLTASLETEKFETSETWPIKAYTQALRHEGAELPLVETRVKLAKMMLL
                     RLRGVPERNHKLYRIAVDLTLPLFSNKASRRLFLVVVREFYHFWIGNPDAASKVLQDG
                     SGNILL"
     gene            8229..9419
                     /locus_tag="PKL42_05780"
     CDS             8229..9419
                     /locus_tag="PKL42_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNU66621.1"
                     /translation="MQALITSSDVLFVLLGAMMVLAMHAGFAFLEVGTVRKKNQVNAL
                     VKIMVDFSVSTIAYFFIGYSIAYGVDFFSGAETLSVNNGFALVKFFFLLTFAAAIPAI
                     ISGGIAERAKFNPQMAATFIVVGFIYPFFEGIVWNEHYGVQSWLLATFGAKFHDFAGS
                     VVVHAMGGWIALAAVVLLGARNGRYSKDGRLHAYPPSNIPFLALGAWILTVGWFGFNV
                     MSAQSSQGISGLVAVNSLMALVGGTLAAFIAGKNDPGFVHNGPLAGLVAVCAGSDVMH
                     PLGALLTGVVAGALFVMAFNLTQSRLKIDDVLGVWPLHGLCGAWGGIAAGIFGSTIFG
                     GRGGVSFFSQLIGTSLGIVIALVGGVMVYGVLKKLVGIRMGQEEEFDGADLSIHKIGS
                     TAED"
BASE COUNT         2620 a         2007 c         2310 g         2723 t
ORIGIN      
        1 ctggtcacct aaagctttgc ggggcgggca gggcgcgcag tttgtattgc ctattgtgga
       61 acgcgctgat ttggtaacag tgctaaacca ttttcaagga aacagttatg ccaccaccat
      121 gcaaggcgaa tcgctctacc aacaaaactt atctgcaccc actgcatttg tcattggcaa
      181 tgaaggtgca gggctaaaac ataaaaccat tgcggcagcg actaaaagca tgagtgtgcc
      241 catgcaggcc aattctgttg aatcattaaa tgctgctgca gccactgcga tttgcttgtt
      301 tgagcgtgtg agacaattgg cgataaaata ggaaaaatga tgaatcgtaa atgtaaatgg
      361 agcgctggat tttcagccat tgaaatgatg gtggtggttg ccattattgg tattttggcg
      421 atgattgcga ttccaaattc aatggggcgg atcgtgaaag agcaggttaa agcggcactt
      481 cctttaacgg atgttgcaaa agagccgatt gccgcagcgt ggaaagcctt aaaactattg
      541 ccaaaagaca atgaagaagc gggtttgcca gtagctgaaa aaattgtgag caattttgcc
      601 agtgcggtga cggtggagga gggtgcaatt catctcactt ttggtaacaa ggcgcacaaa
      661 gtgctgcaag gaaaagtgat aacgatgcgg ccagcggtga ttgaggagtc aagcattgtg
      721 ccagtggaat gggtctgcgg taacgccaag ccgccagaaa aaatggtcgt aaagggcgcc
      781 aataaaacca atgtagcgga agagtttttg ccgatgctgt gtcgataaaa ttataaagct
      841 acttggctta tacgctgtta atgcgctagt ttaggcgtgt ccaacgttgt ttctgaggcc
      901 agtaattgcg ctgcatccac ggcatcaaac acataagcct gattgcaaaa gtcgcagtgt
      961 acttcaatcg attcacgttc ggctaaaatg ctagcaattt cttcagtccc tagcatacgc
     1021 aacatattgc cgacattttt tcgtgtacaa ctgcacttga attgtgctgt atgaggctca
     1081 aaaatacgta cattttcttc atgaaagagt ctgcgcaata gttcgttagc gtctaactca
     1141 attaactctt gcttggtggt ggtatctgcc agttggcaga ttctattcca tgcatcggtg
     1201 tcaatttcta actgttctgg cagcttttgc aatagcatgc ctgccgcctg attgtgcgcg
     1261 cttgtgagcc aaatgcgtgt ttcaatttgc tcagagcgct gcatataatt ttgcaatatt
     1321 tctgcaatgg tatggccttc aatcggcaca atgccttggt aggtttgccg cccatctttg
     1381 gggtcaagag taattacaaa ctgcccctgt tctaatagcg caataaaatt atcttcaaca
     1441 atatcgcccg tccattttgc cgtagcacga atttcaagct gtgaagtgca ttcaaccact
     1501 agtaatttta gcttgcctcg cccctgaatt tgtagcacca gcgcgccctg cattttaagt
     1561 gtggcagcca gtaacgcgct ggcaaccatt aattcactta aagcggtttt taatagggtt
     1621 gggtaattat ggtgctgcag cgcttgttga attgtttggc tgagctggat gtgattaccg
     1681 cgcacggcgg tgttatcaaa aataaagcgc gagagtaggt cttgattatg catacgtgca
     1741 ttttatcatt cgatgccttc atattgatta ggttaataac aaaaaactgt ttacattgca
     1801 aatgataatc attatcattt attaacttaa tttaaccgag aatggcaaca tggcaaacca
     1861 aattattagt caaacgcaac ttgcgggcga tgcgtcatgc aaagtgcttt ataacaaagt
     1921 gaatgataag gtagaacttg agatcggtgg tacttctata agactagagg ccaaccagtt
     1981 ttttgtgatg catgaaatgc tgcgcaaggc cgtggcgaga ttggtgatgc aaacagatct
     2041 gacaaaagtg tgacatacat gatatttatc aattattcat tgtaatgaga atcgttatca
     2101 tatagaatta aacttagttg agaattattc ttatttaacg ggtgaaaaaa tgcaacatta
     2161 caaatctaat actacacagt ttgcaggtgc taaattaaag acaatttcag catgcgtcat
     2221 ggttttatac gctacacatg caagcttggc gctggctgaa ccagcaagtg aagacaatgc
     2281 gctagatatg caaagtattc ggattattgg cagtcaatct caattattga atattccagg
     2341 ttcagccttg ttaattgagc agcaagattt agaagaaagc catgtaatga ccaccaacga
     2401 agccctgcgc aaagcgcctg gcgtagtggt gcgtgatgaa gagggcattg gcatgcgccc
     2461 gaatattggc atgcgcggcc ttaacccaac acgctcaacc aaagtgctgt tgttagaaga
     2521 tggcattccg gtcacttacg cgccttatgg cgctaatgag agttattacc acccaacggt
     2581 agaccgcttt agtagcattg aagtgcttaa aggcgcatca caaataaaat atgggccgct
     2641 tacggcgggt ggcgtaatta actacattac tccgccagcg ccagaaaaat ttggcggcca
     2701 tttaagtgta actgcgggaa atcgtgactt tttaaatact aaaatgaatg ttggcggcaa
     2761 tggtttatta tttgattaca cacataaaga gggcgatggc gcgcgtgata acacacattc
     2821 aaatgtagaa gatttgaata tcaagtttaa tcaggctctt aacgaccaac atagcattac
     2881 cctacgggca aactggttta gtgaagattc acaagtcagt tactctggcc tcacgcaaaa
     2941 agaatatgaa aactttggcg gtacttacaa cccgtttaat cacgacaatt ttgccaccac
     3001 acgctatggc gtttcagcta cgcatgattg gcaactcaac gacagcgcac tattaaccac
     3061 caatttctat tacagctatt ttgaccgcga ttggtggcgc cagcaaagca acaccaccgc
     3121 gagtaacgtg gccactggca ccggttgcgc agcattttca gcggacagaa ttgccggctt
     3181 ggccactaac ggcgacaatt gccaaggcgt gcaaggacgt ttgcgcactt acaataccta
     3241 cggtgttgag ccacgtttga cgattacgca cgcgctcggt gagcttgaaa tgggcgcaaa
     3301 ggcgcacttt gaggaccaag aacgcaggca agtaaacggc acaaatgcga cggcaagaac
     3361 aggaacattg gttgaaaata atgagcgtaa aactgatgcg tactcaggct ttatttcaaa
     3421 ccgttttact attggccaat tttcaatcac gcccgctatt cgctatgagc atattgaaaa
     3481 cgaacgcacc aatcggttaa cggggagtca tggtagcgca aacctgcaag aatggattcc
     3541 agggattggt gttgcctaca atcccaacca gaacatcacc ctgtttgctg gcgttcatga
     3601 gggctttgcc ccaccaagaa cggaagactt aatcaccaac tcgggcggct ctgtagaggt
     3661 tgatgctgaa aaatccacta attatgaatt aggattcaga gctaaacctg tgaaaggttt
     3721 acaagttgat gctaccgctt tttataatga ttttagtaac ttaattgcgg taggctcagt
     3781 ggcgagtaac gtgagcttgt cgcaaggtga ggccacattt gcgggcttgg aattcagtgg
     3841 tcaatatgac tttgataacg ggttttatag ccgtttagct tacacttggc tgcctgtggc
     3901 cgagcaggat acccctttca aacaagtaag taacggcgcg gccgttgctg gcagttcaaa
     3961 aggcaatcgc cagccctacg cccctaaaaa tacgctgacg gctgcagtgg gttaccgagt
     4021 ggccagttgg aacgcgcaac tggaagcggt gcatgttggc aagcaatatt ctgactttgc
     4081 tgaaacgaat gaggtgccga ataccgcgct gggggccaca ggtcaatttg gtgaaatagc
     4141 cagttacacc atttacaacg cggcggtgaa ctacaagttt gagccgtata aaaccactgt
     4201 ttttgtaacg ggtaaaaact tgtttgataa agactatatt accgacagaa cgcgtggcat
     4261 tttaacggga atgcctagac ttgtgcaggt gggtgtgcgt tacgattttt aggtgctttt
     4321 taatcgcatt taactccatt aaaatcactc gaggaatttt tgatgctctg cgagccttta
     4381 tttataaggc tcgcagagca tcgaaaaagg ttgcaacttg aaaacacgtt tttttacatg
     4441 atttttacct gtttttttaa gtgcgaaagc agcattgatt gagtacataa acagctacat
     4501 tttaacgacc atgtttttga tttttggcac gttttttagc tgccaataag tcatcgccca
     4561 atgccacaag tcagccattg tggcgttttt taattcgtct ggcgggtgtt gccctaaaaa
     4621 tgacagcgct tcaaaaaggc gttgattggc ttggctggca tcgatggatt ttgccagcgt
     4681 ttgtttgctg agtttttcac cagcggcatt acaggccagc gccacgtggg catagtgcgg
     4741 tgtgttgagt tgcagcaatt gctgcaggta aatctgtcgc ggggtggaag caagtaaatc
     4801 tgcgccgcgc acaatgtggg tgatatcttg tgcagcgtcg tccacgacca cggccaactg
     4861 gtaggcaaat aagccatcgg cacgttttaa tataaaatca cccacggctt cattgagctg
     4921 ttggctgagg gttccttgaa ttgcatcttc aaactgaatg ggtgtggcat tggttttaat
     4981 gcgccaagca cttggggcca gtggtttaag tgcgtgtgtc aggcaggttt ttgggtaaat
     5041 aacgccttca ataccccatt gctggctaga atctgtaatt tctttgcgcg tacaggtgca
     5101 ggggtagagc aagtgctgcg cgttcagttg ctgaaaagcc tctgtgtaaa acgcatgacg
     5161 ctggctttgg tagataattt cgccatccca ctcaaaacca aaggcttcaa gctggcgcaa
     5221 aatagtgtct gctgcaccgg caatctcacg tggtttgtcg aggtcttcaa tgcgcagtag
     5281 ccattttcca tggttcactt tggcatcaca atagctggca accgcggcaa ctaacgagcc
     5341 aaaatgcagc ggaccagtgg gcgagggggc gaatctgcca acgtacatgg caagttaatg
     5401 gtctggttgc aggtacggtg gaatatgcca actgctgagt tggttgccca gcaaaattaa
     5461 atggcagggc aggcggtcag cgccattcgc gctcacgtca aattcataag tccgctgcag
     5521 ctgcgcctgc ccacgtgaat tgcgccccag ccacaggttg gagcaggcta cactttcatc
     5581 taacaactgc agctgaaagc gcgcagcaag ctcacgcccc agcaaagtgg cgcgttcgcg
     5641 tttggcgagg ctgtcttgcc aaaaccaaac taccgcaaaa agtatgaata taagcacgag
     5701 ttccattgaa attgattttt tcttggttta actaattgaa tttaaataat tttaaaattt
     5761 agccattgga tcttcatccg gatcttcagg ttcatccaca tggagcgata aacttggttt
     5821 ttcaaacgcg ctggattttt cgcctgcatt tttgctatgc aatttaaaac gcaaacgcag
     5881 ttcgttaatg gaatcggcat tgcgaagcgc gtcttcttgc gtgatgcggc cttcttcgca
     5941 taagttgaac aaggcttggt caaacgtttg catgccaagt tcaactgatt ttgccatcgc
     6001 ttctttcacc atgttggttt cacctttaag aattaaatcg gctacaagtg gtgaattgag
     6061 caaaatttct atggcggcac aacgcccacc acccgtgcgt gaaaccaagc gttgtgaaac
     6121 aaacgctttc atgttgagcg ataagtccat gtgtagttgc gcatggcgtt cttcagggaa
     6181 gaagttaata atgcggtcaa gcgcttgatt tgagctattg gcatgtagcg tggccataca
     6241 aaggtggcca gtttcagcaa aggcgagggc aaattccatg gtttcgcggt cgcgaatctc
     6301 gccaattaaa atcacatcag gcgcttggcg caaggtgttt ttaagtgcat tgaaccaacc
     6361 gttagtatca cggcccactt cgcggtgggt aattaaacag tttttgctgg tgtgtacata
     6421 ttccacaggg tcttctacgg taatgatgtg cccatagcta ttttggttgc gataatcaat
     6481 caatgccgcc agcgtcgtag atttacccga gcccgtaccg ccgactaaaa tcaccaagcc
     6541 acgcttagtc atcatcacgt ctttaagtac ttcgggtaag cctaggtcat caaaattagg
     6601 gatttgcgtg gtgatggtac gcaacaccat gccgactttt tcctgttgca caaacacgtt
     6661 cacccgaaag cgaccaatcc cagcagggta aatcgcaaag ttacattctt tatcaacagc
     6721 aaactcagca cgttgctttt cattcatcag cgcatctgca aatgctttgg tgaggtctcc
     6781 cgtgagtttt tgttgtgtaa cgggggtcat ttttccgtca attttcatag cgggaggaaa
     6841 atcagcagaa ataaataaat cagaagcttt tttatcgagc atgccacgca ataaattatg
     6901 catgtaattt aaggcttgtt catttgtcat tcaatcaaac tccctgcgtt attgttatta
     6961 tctctatgca tcttttgcct agagcgacca ttgcaatgtt aaaataaaag gctgaaaatt
     7021 gtgcttaaag gcaaattgta atgcataaaa aacattatga tgttgaatta taagcaaagc
     7081 aaaccgtaat tacggttcca gatatgtcat tgtgcgttag gaaaagctaa aatttaatga
     7141 gtcaaatgag agaaaaagaa gccattgatg cctattttaa gttggtgcag gcaaagggcg
     7201 ctgataatgc aacactcaaa aaacgtgtat tgtttttaga gcaattaagc gagcatttag
     7261 cagaaaaaaa tgtcactggc caagattatc gtgaggctgt tgaagcgctg atggaaacga
     7321 ttccgcccga agactggcac ccaagcctga ccacagcgcg tgagtacttt ccattttgga
     7381 tgcatgacat caaagccatt gctgcccaca accgttttcc tggctttgat attccaccaa
     7441 atcaatggcg gccagcgacc actaccttga aaacgctgac cgctagccta gaaactgaaa
     7501 agtttgaaac ttcagaaact tggccgatta aagcctacac gcaagccctc cggcatgaag
     7561 gtgcagaact gcccttagta gaaactcgcg ttaaactcgc caaaatgatg ttgttacgat
     7621 tgcgcggcgt gccagagcgc aatcacaaac tataccgcat tgcggtagat ttaaccctac
     7681 cgttatttag caacaaagcc agcagacgcc tatttttagt ggtcgtgcgc gagttttacc
     7741 acttttggat tggcaaccca gatgccgcca gtaaagtgct acaagatggt tcaggcaata
     7801 ttctgcttta aataatccca atctaaaaat caacttaagc aaaacttcgc gcgcaatttg
     7861 agtattttag aaatgcttaa atacattaaa ttcaagcatc aaatctcccg agaaaaaata
     7921 agtgctctgg aagcctttat ttataaggct tgcagagcat caaaaaaggt tgtgtgttaa
     7981 aaatatgcca ttttgctatt tttttaataa tttctgcatc atttttttgc acttaataaa
     8041 gcatcttttg cgaccattgt taaattgcac caattcagtg ctaacggtgg tgcaaagcgc
     8101 ttttaaagtg catttcggtt aacctaaagt ttttaaataa cataaaaaac aatgagttaa
     8161 gtgttggctt gtttcttgcg taacctcgcc attacgattt ttttactcat tttggggaga
     8221 cttcagttat gcaggcgtta atcacatcca gcgacgtttt atttgtattg ttaggcgcca
     8281 tgatggtgct ggcaatgcat gcaggttttg cctttttaga agtgggtacg gtgcgcaaaa
     8341 aaaatcaggt gaatgccttg gtgaaaatca tggtggattt ttcggtttct acaatcgcct
     8401 atttttttat tggttacagc atcgcttacg gggtggattt ttttagcgga gcagaaacgc
     8461 tatcagtcaa taatggcttt gctttggtga agttcttttt cttactgacg tttgctgccg
     8521 ccattccagc cattatttct ggcggcattg cagagcgtgc aaaatttaac ccgcaaatgg
     8581 cggccacttt tattgtggtg ggctttattt atcctttttt tgaaggcatc gtctggaacg
     8641 aacactacgg tgtacagtca tggctattag ccacttttgg ggctaagttt catgattttg
     8701 ccggctcagt agttgtgcat gccatggggg gctggattgc gttggctgcc gtggttttgc
     8761 tgggtgcacg aaatggacgt tatagtaaag acggccgcct gcatgcatat ccaccatcga
     8821 acattccatt tttagcttta ggtgcatgga tattaaccgt tggctggttt ggttttaatg
     8881 tgatgtccgc acaaagtagc caaggcattt caggcttggt ggcagtgaat tctttgatgg
     8941 cgctcgttgg cggcacgctg gcggcgttta ttgcaggtaa aaatgaccca ggctttgtac
     9001 acaatggtcc actcgctggc ttggtagcgg tttgtgctgg ctccgatgtg atgcatccgc
     9061 ttggtgcgct gctgacggga gtggttgctg gggctttatt tgtgatggcg tttaatttaa
     9121 cgcaaagccg tttaaaaatt gatgacgtat taggcgtgtg gccattacat gggctttgcg
     9181 gagcatgggg cggcattgct gctggcatat ttggcagcac catttttggc ggccgaggcg
     9241 gcgttagctt cttctcgcaa ttgattggaa cgagtttggg tatcgttatt gcgcttgtgg
     9301 gtggcgtaat ggtgtacggt gtgcttaaaa aactagtagg tattcgcatg ggccaagaag
     9361 aagagtttga tggtgcggat ttaagtattc ataaaatagg ctcaacagcg gaagattaat
     9421 cgtgtgacgc tcttttgtga tttatttgat tgaaactaac ccttgtgggt gattgaactt
     9481 aattttataa agttgtattg ttattattca ataagttata aactttaatt cataaaaaca
     9541 tatccgttag atacttcgta aaaatatctg gagtgttggt aaataggttc aatatgaaaa
     9601 aattagtttt gctatcatta atgtcggctt ttacttcatc tgcatccgca gcagttgtat
//
LOCUS       DAOQSI010000069         9658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_6435_length_9658_cov_4.808081, whole genome shotgun sequence.
ACCESSION   DAOQSI010000069 DAOQSI010000000
VERSION     DAOQSI010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 9658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9658
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6435_length_9658_cov_4.808081"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PKL42_05785"
     CDS             complement(<1..192)
                     /locus_tag="PKL42_05785"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNU66622.1"
                     /translation="MNIKSIITLLIVYLMAMTASIASAEVPQGYKLRHGDLIQVSVWG
                     EEKLHKESLVLPDGSITYPL"
     gene            complement(272..556)
                     /locus_tag="PKL42_05790"
     CDS             complement(272..556)
                     /locus_tag="PKL42_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66623.1"
                     /translation="MSKKIAIIALLSLSISLSNISFAAVADEASTPVVTSNVSIKDQA
                     LKQTVNGTEVVNKRLNEPTDRDQEMPLPATGWLLFFSLIGFVLLSNRQNI"
     gene            complement(806..2026)
                     /locus_tag="PKL42_05795"
     CDS             complement(806..2026)
                     /locus_tag="PKL42_05795"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNU66624.1"
                     /translation="MSKVNKVVLAYSGGLDTSVILKWLQDEYQCEIVTFTADLGQGEE
                     LEPARAKAKAAGVKEIYIDDVREEFVRDFVFPMFRANTVYEGEYLLGTSIARPLIAKR
                     LIEIARETNADAISHGATGKGNDQVRFELGAYALNPNIRIIAPWREWDLLSREKLMAY
                     AEAHGIPVDMKHKQGGSPYSMDANLLHISYEGRHLEDPAAEAEESMWRWTVSPENAPD
                     AAEYLDIEYVNGDPVSLNGEAMKAHEILAHLNKLGGKHGIGRLDLVENRYVGMKSRGC
                     YETPGGTILLKAHRAIESITLDREVAHLKDDLMPRYASLIYNGYWWSPERLALQTLID
                     HTQKSVNGWVRVKLYKGNVIVSGRDSETDSLFDSSIATFEDDKGAYDQKDAGGFIKLN
                     ALRMRIAANLKNRK"
     gene            complement(2030..2944)
                     /gene="argF"
                     /locus_tag="PKL42_05800"
     CDS             complement(2030..2944)
                     /gene="argF"
                     /locus_tag="PKL42_05800"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNU66625.1"
                     /translation="MAIKHFLQFNDLNRDELEHLFSRTKIIKQQFKAYQQYWPLQDRT
                     LVMIFEKASTRTRLSFEAGMHQLGGSAIYLNTRDSQLGRGEPVEDAAQVISRMSDLVM
                     IRTFEQEIIERFAANSRVPVINGLTNEYHPCQILADIFTFIEHRGSMKGKTVAWIGDS
                     NNMCNTWLQAAELLDFNVHVSTPPGYEVEPERANLYGHDHFEVFADPMDAAKGADLVT
                     TDVWTSMGFEAENEARKRDFADWQVDSDMMHIAANDALFMHCLPAHRGEEVAADVIDG
                     AQSVVWEEAENRLHVQKALMEYLLLGKV"
     gene            complement(3005..4186)
                     /locus_tag="PKL42_05805"
     CDS             complement(3005..4186)
                     /locus_tag="PKL42_05805"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HNU66626.1"
                     /translation="MTQHLMNTYSRQPVTFVKGEGVWLWDNQGEKYLDALAGVAVNGL
                     GHAHPKLVKAISEQAGKLIHVSNIYQIAEQAALADKLCEISAMDKVFFCNSGCEANEA
                     AIKLARLYGHNKGIENPEIIVMDKSFHGRTMATLSATGSRKVQAGFEPLVSGFVRVPY
                     DDVEAVKQVATRKNNVVAILVEPVQGEGGINIPKDAAGYLEALREICDANGWLLMLDE
                     VQTGIGRTGTWFAFQHTSIKPDVMTLAKGLGSGVPIGACVARGVAADVFTYGKHGSTF
                     GGNPLATAAGLATLKIIEEEHLRENAEKIGNIMRESFLAELKTTQGVVTVRNAGLMIG
                     IELDRPCGDLVKMALEDHLLINVTAEKVVRLLPPLIMNQAEAQILVSRLSALIKAFLT
                     K"
     gene            4364..5500
                     /locus_tag="PKL42_05810"
     CDS             4364..5500
                     /locus_tag="PKL42_05810"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HNU66627.1"
                     /translation="MTRTNALRAQTQASLTQTQLPVACYVDAKVYALEQQHLFSQMPK
                     YIGHELMVPNVGDFRVLDWANEASSLVHDSASLHLISNVCRHRQAIMLKGHGNTKNIV
                     CPLHRWTYNLQGELLGAPEFDKNPCLHLNRKTLQNWQGLLFESERNIAEELNQIGCKT
                     DFDFTGYQLHSVDIEHYNFNWKTFIEVYLEDYHVGPYHPGLNQFVDCGDLAWDFGEHY
                     SVQTVGFRAMPHQHATPAYQRWQKAVRNYRQASHTKTATNHGAIWMVYYPLLMVEWYP
                     NVLVVSHIIPQGVDACMNVVEFYYPEDIALFESEYVAAQKAAYHETAIEDQDICQRMH
                     DGRRALFAQGLDERGPYQHPMETGLAHFHQWYRAQLAPHIALLN"
     gene            5532..6419
                     /locus_tag="PKL42_05815"
     CDS             5532..6419
                     /locus_tag="PKL42_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNU66628.1"
                     /translation="MANLLPKYDLPKLGSLWMLVAAAGFAIMGALVKVGAQKFSSGEL
                     VFYRSLFGLLVIWLYILQQKKPLATPYLGKQVSRALVGFVSLVLFFYAIAHLPLATAI
                     TLNYTSPLFLAILTPFLLHEPPKKTLALTLIMGFIGISLLLKPTLDSDDWLAGSIGLL
                     SGVGAAWAYVHVKQLGNLNEPDWRTVFYFTLISTIGAGGWMLFSDFHSVDWQDLPLLL
                     GLGLSATIAQLAMTRAYRTGNTLVVASLAYLTVVLASLIGVFWWHEHLNLESWLAIGL
                     IILSGIISVRSTQVQSAKK"
     gene            6815..>7320
                     /locus_tag="PKL42_05820"
     CDS             6815..>7320
                     /locus_tag="PKL42_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66629.1"
                     /translation="MFQWLVSATLTLLLTGCLGGSIGQQIVQSILLQGADKATASAMD
                     AHERNQIALAKKNAKPDPYTIAFLNSGFEPVTAQIEPLPEAPIIEEEQVLPMMQETKL
                     VPVEIWSLLAGEEKQNVLEAARLRGSTEIPPKDEWRQWQIATGASHQSNQPITFLIPP
                     EMGRMRSGA"
     assembly_gap    7321..7420
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7611..8249)
                     /locus_tag="PKL42_05825"
     CDS             complement(7611..8249)
                     /locus_tag="PKL42_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNU66630.1"
                     /translation="MSSIHVMLVDDHAVVRMGFKMLLESDSDIKVVAEAESGEQAIQR
                     FVEFKPHVVVMDITMPGIGGLEAIERILAKDNTAKILVLSAHEDSVHPKRVLNAGAMG
                     YLTKRSAAEELIKAIRVVATGKKYLEASVAQQMAIQQLSGEQNPVDVLSPREFEVFMA
                     LAKGKTTNEIAETLFLSPRTVGTHLYNIKQKLNANNSAEIALIAMRSGLLDA"
     gene            complement(8283..9632)
                     /locus_tag="PKL42_05830"
     CDS             complement(8283..9632)
                     /locus_tag="PKL42_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNU66631.1"
                     /translation="MSLKFRLNLLITLLSIAFILVVGSILVNDTRISIRERVEAASRV
                     TVQLLDTVIVSSALNPEYGPTHKVLQSFLQSLGYVRSSHIVLKDLRDEIIYESPESTY
                     KDEVKPPDWFVHLVAPATEKVERKIRYGTLIVTSSAAGSIREAWSGFSQLMMVGLGFF
                     VVLNVMVYALLSHSLRPVSRILKAINKIERGDLTTRLPAFKLPEFDRIGHSLNRMAES
                     LAVERQLEENRQLTQLIQSHIEDERRSLARELHDELGQYVTAIKTFAVAIVNKTREKS
                     PDIASNAQTIVAAANQIYDGMHDIIRKLRPGALDNLGLAETLRDAVNHLHSQHPALNI
                     NLQIEGELDDLGETLNINLYRIVQEAMNNALKHAQASKIDIKLAVNTDEYEQKNIVLI
                     IADDGVGMNLAAVDQTQHFGLLGMRERVQGFHGSFNLISEPNQGTTLNISIPLRVAP"
BASE COUNT         2637 a         2294 c         1919 g         2708 t
ORIGIN      
        1 aagtgggtag gtaatgctac catctggtaa aaccaaactt tctttatgta atttttcttc
       61 accccaaact gatacctgaa ttaaatcacc atgacgtaat ttatagcctt gtggcacctc
      121 agcacttgca attgatgcgg tcattgccat caagtaaacg atgagtaaag taataattga
      181 tttaatattc atttgcattt cctttaaaat ttatatgctc tatgtgagcc tttattttac
      241 ttcttgattt aaaattttca gattaaatca cttaaatatt ttgcctattt gacagcaaaa
      301 caaatcctat gagtgaaaag aacaacagcc atccagtggc tggcaacggc atttcttgat
      361 cacgatctgt cggttcattc aaacgcttat taacaacttc agtaccattt actgtttgct
      421 tgagcgcttg atcttttatc gagacatttg atgtcacaac tggcgtactt gcttcatctg
      481 caactgctgc aaaagaaata ttggacaacg agatacttaa actcaacagc gcaattatcg
      541 caatcttttt tgacatttgg cttgcctttc aacatacatg agtaacattt actaactggt
      601 actcattcta tgaagggcaa gccttggtca caatagccca taagggccat caaaattatc
      661 aagcattaaa aattaaattt taaacttaat aaatcaataa gttataaaat ataattcaat
      721 taaccatgca ataatattcc gcctatttta caaagtaaaa acataaaagc atttatatct
      781 aagcttcaaa tttattgaaa aaattctatt ttctattttt tagatttgca gcaatacgca
      841 tcctaagtgc atttaattta ataaacccac ccgcatcttt ttgatcataa gcacccttgt
      901 catcctcaaa agtcgcaatc gatgaatcaa acagcgaatc cgtttcacta tctcggccag
      961 aaacaattac attaccttta tagagtttca ctcgcaccca accattcact gatttctgtg
     1021 tgtgatcaat taatgtttgc aaagcaagtc gttccgggct ccaccagtag ccgttgtaaa
     1081 ttaaactagc gtagcgtggc atcaaatcat cctttaaatg ggccacttca cgatcaagcg
     1141 taattgactc aatggcgcgg tgtgctttta acaaaatagt accacctggt gtttcgtaac
     1201 agccgcgtga cttcatcccc acgtagcgat tttcaaccaa gtctaggcgg ccaataccgt
     1261 gcttaccacc caacttattc aaatgtgcca aaatctcgtg cgccttcatg gcttcgccat
     1321 tcaaacttac tgggtcgccg ttcacatatt caatatctaa atactctgca gcatctggtg
     1381 cattttctgg actgactgtc cagcgccaca tagactcctc agcctctgcg gctgggtctt
     1441 ctaggtgacg gccttcgtat gaaatatgta ataaattggc atccatactg tatgggctgc
     1501 cgccctgctt gtgcttcata tccacaggaa taccatgcgc ttcggcgtaa gccatgagtt
     1561 tttcacggct gagcaaatcc cattcgcgcc aaggggcgat aattcggatg ttaggattta
     1621 acgcatacgc gcccaattca aagcgcactt ggtcgttacc cttgcctgta gcgccatgtg
     1681 aaatggcatc cgcattggtt tcacgcgcga tttcgattaa acgctttgca ataagcggac
     1741 gtgcgataga ggtgccgagc aggtattcgc cttcatagac cgtattggcc cgaaacatgg
     1801 gaaatacaaa gtcacgcaca aactcttcac gcacatcatc aatgtagatt tctttcacgc
     1861 cagccgcttt tgcttttgca cgcgccggct ccagttcttc accttgacct aaatcggcag
     1921 taaacgtgac gatttcacat tggtactcat cttgcagcca cttcagaatc actgaagtat
     1981 caaggccacc tgaatacgct aaaacgactt tattaacttt gctcattggt tacacttttc
     2041 ctaataataa atattccatc aaggcttttt gtacgtgcaa gcgattttcc gcttcttccc
     2101 acaccacgct ttgcgcgcca tcaataacat cggcagccac ttcttcaccg cgatgcgcag
     2161 gtaggcaatg cataaatagc gcatcatttg ctgcaatatg catcatgtca ctatcgactt
     2221 gccaatctgc aaagtcacgc ttacgggctt cattttcggc ttcaaacccc atactggtcc
     2281 acacatctgt agtgactaaa tctgcacctt ttgcggcatc catcgggtcg gcaaacacct
     2341 caaaatggtc atgaccgtat aagttagcgc gctcgggctc tacttcataa cctggtgggg
     2401 ttgaaacatg cacattaaaa tctaacaact cagcggcttg caaccaagtg ttgcacatgt
     2461 tgttgctgtc accaatccac gccactgttt tgcctttcat tgagccgcga tgttcaataa
     2521 aagtaaaaat atcagccaga atttggcaag gatgatattc attggttaag ccgttaatga
     2581 ctggcacacg tgagtttgcg gcaaagcgct caataatttc ttgttcaaag gtccgaatca
     2641 tcaccaaatc actcatgcgt gaaatgactt gcgctgcatc ttcaaccggt tcaccacgac
     2701 ccaattgcga atcacgggtg ttcaaataaa ttgctgagcc accaagctgg tgcatccccg
     2761 cctcaaacga aagtcgtgtt ctggtgctgg ctttttcaaa aatcatcacc agtgtacgat
     2821 cctgcaaagg ccaatattgc tgataagcct tgaattgttg cttaatgatt ttggttcgcg
     2881 agaatagatg ctctaattca tcacgattga gatcattaaa ttgtaaaaaa tgtttaatag
     2941 ccatataagt taagtcgaat aattttatta aaactagcga ctaattttga tttttcatga
     3001 aaatttattt tgttaaaaac gctttaatta aggctgacag gcgcgaaact aaaatctgcg
     3061 cctctgcttg attcatgatt aaaggtggaa gcaagcgcac cactttttcc gccgtcacat
     3121 taatcagcaa atggtcctcc aatgccattt tcactaaatc accacaaggg cgatccaact
     3181 caataccaat catcaaacct gcatttctaa ctgtcaccac gccttgcgtg gttttaagct
     3241 cagctaaaaa actttcacgc ataatattac cgattttttc agcattttca cgtaaatgtt
     3301 cttcttcaat aattttgaga gtcgctaaac cggcagcagt tgccaaagga ttgccgccaa
     3361 aggtggagcc gtgcttgcca taagtaaaca catctgccgc cacgccacgt gctacacaag
     3421 cgccaatagg cacccctgaa cccaaacctt ttgccaatgt catcacatcg ggtttaatgc
     3481 tggtatgctg aaaagcaaac caagtgcctg ttcgaccaat gccagtttgc acttcatcta
     3541 acatcaatag ccaaccgtta gcatcgcaaa tttcacgcaa tgcttctaaa tagcccgccg
     3601 catctttggg aatattgata ccaccctcgc cctgcaccgg ctcaaccaaa attgctacga
     3661 cattattttt gcgcgttgcg acttgtttaa cggcttccac atcgtcgtat ggtacgcgaa
     3721 caaagccgct gacaagcggc tcaaacccgg cttgcacctt acggctaccg gtggcagaaa
     3781 gtgttgccat ggtccgacca tgaaacgatt tatccatgac gataatttca ggattttcta
     3841 ttcctttatt gtgaccataa agccgcgcga gtttgatggc agcttcattc gcttcacacc
     3901 ctgagttgca aaaaaacact ttgtccatcg ctgaaatttc gcacaattta tcagccaatg
     3961 cagcttgctc ggcaatttga taaatatttg aaacgtgtat gagtttgcct gcttgctcgc
     4021 taatcgcttt tacaagctta ggatgcgcat gacccaagcc gttcaccgca acgccagcca
     4081 gtgcgtcaag atacttttca ccttggttat cccaaagcca aacgccctct cctttgacaa
     4141 aagtcactgg ttggcgcgaa taagtgttca ttaagtgttg agtcatagtt cagaatagtt
     4201 cagatttaca ggtgtttaac cttgcgagat tagcttaaaa cctcgctttt ggcaagtttt
     4261 agcttttggt ttactggtca atgacactaa ataattgctt gaaaaaaagt gacttttagg
     4321 caaaaatcct ccctctttta aattacacct cgttttattg cttatgactc gcactaacgc
     4381 tcttcgcgcc cagacacagg ccagtctcac acaaacacaa ttacccgttg cttgctatgt
     4441 agatgccaaa gtatatgcct tagagcaaca acatttattt agtcaaatgc cgaagtatat
     4501 tggccatgaa ctgatggtgc ccaatgtggg tgattttcgt gtacttgatt gggcgaatga
     4561 agccagcagt ttagtgcatg atagcgcaag cctgcatctc atcagcaatg tttgccgcca
     4621 tcgtcaggcg attatgctaa aaggacacgg caacaccaaa aacattgtgt gcccactgca
     4681 tcgatggact tacaacttgc aaggagagct gctcggcgca cctgagtttg acaaaaaccc
     4741 atgcttgcac ctcaacagaa aaacgctcca aaattggcaa ggcttattat ttgagagcga
     4801 acgcaatatt gccgaggaac tcaatcaaat tggctgtaaa acagattttg actttaccgg
     4861 ctaccaactt cattctgttg atattgagca ttacaacttc aactggaaaa cctttattga
     4921 agtgtatttg gaagactacc atgttgggcc gtaccatccc ggattgaatc aatttgtgga
     4981 ctgtggtgat ttagcatggg attttggcga acattacagc gtgcaaacgg tgggcttcag
     5041 ggcgatgccg caccaacacg ccacccccgc ttaccaacgc tggcaaaagg cggtgcgcaa
     5101 ttaccgtcaa gcaagccaca caaagaccgc taccaaccat ggtgcgattt ggatggtcta
     5161 ctatccatta ttaatggtgg aatggtatcc caatgtgctg gttgtttcgc acatcattcc
     5221 gcaaggcgtt gatgcgtgta tgaatgtggt ggaattttat taccctgaag atattgccct
     5281 ttttgaaagt gaatatgtgg ccgcgcaaaa agcagcctat catgaaactg cgattgaaga
     5341 ccaagatatt tgtcagcgta tgcatgatgg cagacgtgca ctgtttgccc aaggattaga
     5401 cgagcgcggc ccctaccaac acccaatgga aactggttta gcgcattttc accagtggta
     5461 ccgcgcgcaa cttgccccgc atattgccct tttaaactga ttaccctcat tgacggccaa
     5521 ttatcatcaa aatagctaat ttattaccca aatacgacct cccaaagctt ggaagcctgt
     5581 ggatgttagt ggctgctgcg ggttttgcca tcatgggtgc attggtaaaa gttggcgcac
     5641 aaaaatttag tagcggcgag ttggtgtttt atcgctcact ttttggcttg ctggtcattt
     5701 ggttatatat tctgcagcaa aaaaaaccac tcgcaacgcc ctatcttggc aaacaagttt
     5761 ctcgcgcgct ggtgggcttt gtttcactgg tgctgttttt ttatgccatt gcgcacttgc
     5821 cattagcaac ggcgataacg ctcaactaca cttctccctt atttttagcc atcttgacgc
     5881 catttttatt gcatgaaccg cctaaaaaaa ccttagcgct cacgttgatc atgggcttta
     5941 ttggcatcag tttactttta aaaccaacgc tagactcgga tgattggctg gcaggcagca
     6001 ttggtttact gtccggcgtg ggcgcggcgt gggcttatgt acatgttaaa caactgggca
     6061 atctgaatga acccgattgg cgcacggtct tttatttcac acttatttca acgataggcg
     6121 caggtggctg gatgttattt agcgactttc attccgttga ttggcaagac ttacccttac
     6181 tactgggctt aggcctttct gccaccatcg cacaactagc catgacgcgc gcctaccgca
     6241 cgggcaatac gttagtggtg gcaagtttgg cctatttaac agtagtgctc gccagcttaa
     6301 tcggtgtatt ctggtggcac gagcacctta accttgagtc atggttagcc attggcttaa
     6361 ttattttaag cggcatcatc agtgtgcgta gcactcaagt gcaatcggcc aaaaaatagt
     6421 gctacttggc atgtgattca aggctattta aatcctcatt taacagctta attttctacg
     6481 agaaaaaatt tatgccctgc gagcctttgt ttacaaggct tccagagcat caaaaaaggt
     6541 tggtgttaaa aattggctaa aaatacctat tgttaatgcc tgaaattctg ataaaaaatt
     6601 gttcttttta ccccaattaa ataatggaat atgaacgcta tcgttttagc tcaaatacaa
     6661 caaaccgata cgcaccagtg atgcaacctc tttaaacccc tatacaaacg cactatttag
     6721 aggtaatctt atcttccgca tttaactgat tgattgtatt gaatttacaa taacagaaat
     6781 gacaccgcaa cgagacaaaa taaagtgttt tgcaatgttt caatggttgg tatctgcaac
     6841 cttgaccctg ttgctgaccg gctgtttagg cggctctatc ggccagcaga ttgtgcaaag
     6901 cattttgcta caaggggcag ataaagccac cgccagcgcg atggacgccc atgagcgtaa
     6961 tcaaattgcc ttggctaaaa aaaatgccaa gcccgaccct tataccattg cgtttttaaa
     7021 ttctggcttt gaacctgtca ccgcgcaaat tgagcctttg cctgaagcgc cgataataga
     7081 agaagaacaa gttttaccca tgatgcagga aaccaaactg gtgcctgtgg aaatttggag
     7141 tttgcttgcg ggggaagaaa agcaaaacgt gctggaggcc gccagactgc gcggctccac
     7201 agaaattccg cctaaagatg aatggcgcca atggcagatt gccacgggag catctcacca
     7261 atcgaatcaa cccattacat ttttaattcc acctgaaatg ggcagaatgc gaagcggcgc
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atcaacccat tacattttta
     7441 attccacctg aaatgggcag aatgcgaagc ggcgcaaagg cgatggtaga gttatctcgc
     7501 tcaaatgaac taagcgtagc gcgctacagt ttgaattaag cttaacttat taaaataaca
     7561 gtcaaatcta gcgctttatt gagtgtaaat catctcagac caaaatcata ttaggcatcc
     7621 agcaaaccac tgcgcattgc aatgagcgca atttctgccg aattattggc gttcaacttt
     7681 tgtttaatgt tatacagatg cgtacccacg gtgcgcggac tcaaaaacag agtttcggct
     7741 atttcattgg tggttttacc tttggcaagc gccataaaca cttcaaactc gcgcggcgac
     7801 aacacatcca ctgggttttg ctcaccagaa agctgctgaa tcgccatttg ctgcgccacg
     7861 ctggcctcaa ggtatttttt gccagtcgcc accacacgaa ttgccttaat gagctcttct
     7921 gcggcactgc gttttgttaa gtagcccatc gccccggcgt tcagcacccg cttggggtgt
     7981 acagaatctt catgcgcgga gagtaccaaa attttggccg tgttatcttt tgctaaaatg
     8041 cgctcaatcg cctctaaacc gccaatgccc ggcatggtga tatccatgac caccacatgc
     8101 ggcttaaact caacaaaacg ctgaatcgcc tgctcgccgc tttcagcttc tgccactact
     8161 ttaatatccg aatcagattc gagcagcatt ttaaagccca tgcgcaccac cgcgtggtcg
     8221 tcaaccaaca ttacatgaat tgaactcatt taattatcct gtgtttgcaa tatttattca
     8281 cattaaggcg ccacacgtag cggtatgctg atattaagtg tggtgccttg attcggttct
     8341 gaaatcaaat taaaactacc atgaaagcct tgtacacgct cgcgcatgcc caataatcca
     8401 aaatgctgtg tttggtcaac ggccgccaga ttcatgccta caccgtcatc tgcaatgatg
     8461 agtacaatgt ttttttgctc atattcatcg gtgttcactg caagttttat atctatttta
     8521 ctcgcttgtg cgtgttttaa tgcgttattc atggcttctt gcacaatgcg gtataaattg
     8581 atattgagcg tttcaccgag gtcatccaat tcgccctcta tttgcaaatt gatgtttaac
     8641 gcaggatgct ggctgtgtaa atggttgacc gcatcgcgca gggtttcggc tagccctaaa
     8701 ttatcgagtg cacccggcct taatttgcga ataatatcgt gcatgccatc gtaaatttga
     8761 tttgccgcag caacaatggt ttgcgcatta ctggcaatat cgggcgactt ttctctggtt
     8821 ttattcacaa tcgccacggc aaaagtttta atcgcggtga cgtattgccc tagttcatcg
     8881 tgcaactcgc gcgctaaact gcggcgctcg tcttcaatat ggctttgtat gagttgcgta
     8941 agctggcgat tttcttccaa ttgccgctca accgcaagtg actctgccat gcggtttaaa
     9001 ctgtgaccga tgcggtcaaa ttcgggtaat ttaaaggctg gtaaacgcgt ggttaaatca
     9061 ccccgctcaa ttttattaat ggcttttaaa atacggctca ctggccgcag tgaatggctt
     9121 agcaacgcat ataccatcac attcaatacc acaaaaaagc ctaaacccac catcatcaat
     9181 tgactaaaac cagaccaagc ttcgcgaatc gaacccgcgg cacttgaagt gacaatgagc
     9241 gtaccgtaac gaattttacg ttctactttt tctgtggcag gcgcaaccaa atgcacaaac
     9301 caatcaggcg gttttacttc gtctttatag gtggattctg gcgattcata aatgatctca
     9361 tcgcgcaaat cttttagcac aatatggctg ctacgcacat aacctagtga ttgtaaaaaa
     9421 ctctgcagta ctttatgcgt ggggccatat tcaggattaa gtgcggaact cacaatgacg
     9481 gtatcgagca attgcacggt gacacggcta gccgcttcta cccgctcgcg aattgaaatg
     9541 cgcgtatcgt tgactaaaat cgagccaacc accagaataa atgcaatcga cagtagcgta
     9601 atcagcaaat tgaggcgaaa ttttaagctc atacgtgtgc ctcaatatgt gcgctaat
//
LOCUS       DAOQSI010000070         9612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_6480_length_9612_cov_4.830281, whole genome shotgun sequence.
ACCESSION   DAOQSI010000070 DAOQSI010000000
VERSION     DAOQSI010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 9612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9612
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6480_length_9612_cov_4.830281"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..133)
                     /locus_tag="PKL42_05835"
     CDS             complement(<1..133)
                     /locus_tag="PKL42_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HNU66632.1"
                     /translation="MIATMQHNIEIIPIPAFENNYIWLIHNGKDAIVVDPGDANPVIE
                     "
     gene            151..912
                     /locus_tag="PKL42_05840"
     CDS             151..912
                     /locus_tag="PKL42_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNU66633.1"
                     /translation="MSIETRPDSDWFNGTAGRYLLSQEQAWYDNEVFDLFGFNALQMG
                     CLHMDLLRNSRIPNRYLSADSVTATQPHHISCCDDFLPFADASLDLLVLPHRLEFSER
                     PHQTLREAARVMMPEGHLLISGFNPTSTWGATARMNAWFCKNKTYPWNGEFIGLTRLK
                     DWLALLGFEVMWVDKRCHIPPFEQASWHKRFTFMDKLGARCGGVYLIVAKKRVVGMTP
                     IKPNWKAHPLKSALVARRPKSNTSQNTTSQFDATK"
     gene            complement(1103..1375)
                     /locus_tag="PKL42_05845"
     CDS             complement(1103..1375)
                     /locus_tag="PKL42_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66634.1"
                     /translation="MHIVKQFILVAAIASAVVTGVAVAEDAAVTCATTGQPAQLEGKV
                     INVDTTNEKITVRDSNGTIHEFQASVETLQEYKAGDTIKAKLRCEK"
     gene            complement(1387..1617)
                     /locus_tag="PKL42_05850"
     CDS             complement(1387..1617)
                     /locus_tag="PKL42_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HNU66635.1"
                     /translation="MNKTSQLISKKSKLTVITSLTLVTLLATACGTVTGAAVGAGAGA
                     AVGAGTGHGAKKGALIGTGIGAAVGAVYDITK"
     gene            1890..2366
                     /gene="rnhA"
                     /locus_tag="PKL42_05855"
     CDS             1890..2366
                     /gene="rnhA"
                     /locus_tag="PKL42_05855"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HNU66636.1"
                     /translation="MSSQAIGSKTEVEIYADGACKGNPGPGGWGAWIRYGSKEKELFG
                     GELLTTNNRMELTAVIRALEALNRTCHAKVFTDSVYVQKGISEWIIGWKARNWRTADK
                     KPVKNDDLWRVLDTLAQQHTVQWVWVKGHAGNVGNERADMLANLGVAQVLQNENEK"
     gene            2363..3064
                     /gene="dnaQ"
                     /locus_tag="PKL42_05860"
     CDS             2363..3064
                     /gene="dnaQ"
                     /locus_tag="PKL42_05860"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HNU66637.1"
                     /translation="MRQVFLDTETTGLYHAQGHRVIEVAAVEVVNRRLTKQHFHYYLN
                     PDREIDQGAQEVHGISLEFLQDKPRFADIAHDLIAFIADAELIMHNAPFDVGFLNMEF
                     GLIDQKPVESIAAKITDTLKIAKEMRPGQRNSLDALCKHFGIDNSKRTLHGALLDAEL
                     LADVYMAMTRGQDSLMMELNQPQISMEARALQQNVPLMIKRVSTEEETAHQEYLAALA
                     KSGACLWQKLKEEMP"
     gene            3126..4211
                     /gene="rfbB"
                     /locus_tag="PKL42_05865"
     CDS             3126..4211
                     /gene="rfbB"
                     /locus_tag="PKL42_05865"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNU66638.1"
                     /translation="MNQTIIVTGGAGFIGANFVLAWVKQGLGTVVNLDKLTYAGNLEN
                     LVDIQQNPQHIFVHGDIGDQALVSKLLAEHKPCAVVNFAAESHVDRSIHGPEDFIQTN
                     VVGTFHLLESVRAYWGALNETDKKAFRFLHVSTDEVYGSLSKDEPAFVETRKYEPNSP
                     YSASKAASDHLVRAYHHTYGLPVLTTNCSNNYGPHHFPEKLIPLVIHNALAGKPLPIY
                     GNGQQIRDWLYVQDHCSAIRRVLEAGTVGEVYNVGGWNEKPNIEVVKTLCKMLDAKKP
                     RADGKPYADQITYVEDRLGHDQRYAIDATKIARELGWKPQETFESGIEKTVNWYLEHQ
                     EWVNNVTSGDYRNWVQKHYVDRGEEMA"
     gene            complement(4353..4793)
                     /gene="queD"
                     /locus_tag="PKL42_05870"
     CDS             complement(4353..4793)
                     /gene="queD"
                     /locus_tag="PKL42_05870"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HNU66639.1"
                     /translation="MQITTRLEFDAGHRIPCHKSQCKNLHGHRYAIEITLSGDIIKNE
                     HASENGMVMDFSDVKAIAKSALVDVWDHAFLVYQHDTDVLNFLNSLPQHKTVVFPTVP
                     TAENMAAEAFKILKTKYQDTYGNHLKLEKVRLYETPNNWADALS"
     gene            complement(4904..6757)
                     /locus_tag="PKL42_05875"
     CDS             complement(4904..6757)
                     /locus_tag="PKL42_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNU66640.1"
                     /translation="MANQSSLALTEAKPSQVATAPAFSFALSPNELVQLMNTYGLKAS
                     DVVGLYQVSSALFHPLRSLPPFNTMDAAEANHEKSIDVIRQWPKQLNWVQFNTMINRL
                     LQKWLEDYQAGAALANAFVLARSLTLQTAEAIGTQDLQHADQTVSLKFQSFLMHLLAT
                     ATHQFDAFQFNYIQHYDLETGLPNQRFLLDILNRYSPVTEAAVAQANDVLASEKIGIL
                     VIQLNINFDKTPQLNAISNQIVRAAVDVVEQHLSSDATLFRIGVFEFAIIVENLHFSA
                     QLNLMAAKLAHAFETELPLENVTLILKPIFGGICSLEEPISALSLFDNAKLALHHALV
                     NDSRIEIYDKVISNMHQKAHQLEEAIITALQHNELEMYFQPIVTLPNAENPVERCVSA
                     ELLLRWISEEWPSVSPFRLIETIYKKGFGKVFIRWLINSACRHCAELLSNHQRNVLLT
                     INLCTTDLLDEDLPELLSQSIALWEIPAENLVMEITETDILANEEKVSQVLDQIVSLG
                     CRLALDDFGTGYSSMARLRNMPVDLVKIDQSFVRNIATSNQDKEIVVSILKLAHSLGK
                     EVVAEGVEDPACLQLLRMMKCDKIQGYYYSKPLPFEAFATWLAAFEATHST"
     gene            complement(6750..8363)
                     /locus_tag="PKL42_05880"
     CDS             complement(6750..8363)
                     /locus_tag="PKL42_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNU66641.1"
                     /translation="MSDVSRKETPVSQLEMGMFVTALDRPWLGTPFMLEGLLLEEQEQ
                     IAAMAKLCKVVYVDHTMSIGKHFIAPPKEEIGIKRDGITSKMPISANTKSGLSGTKLA
                     KRSPSSQKFTFFNVMKEIQAANQASLANQANNPGAKTTDNILLNVQNINNQIYTPIIE
                     KDEPEENPSLASKIKEDFTSFIGGLTSWGSKNKKLKDKTDKNNGENADENNSLDQVTI
                     IEEASLVEDEIAEIYPTYDKSQIATREIFEALAAGQQIDITNVNEALDGMVDSIERNP
                     DALMWLAKLKLTDNYAYNHALNVSITLMAFASFMSFPKAQIKNLGMAGLLQDIGKGKL
                     PAQLLHKEGKISSAEFSVLKRHVDYAIDILKETEDIPESVIAIVAEHHERFDGSGYPN
                     ALSGKQINRGGQMAGLIDTYCALTTNKVYAKGVYNQVALERINKMSDVKFSAALVNQL
                     VQFLGMYPVSSLVELNSGEVGVVIQQNSVRRLLPRVMILLNPDKTKNEYPATINLITS
                     PLTPKGEPYKIVRGLPADSYGLNPSNFYG"
     gene            complement(8577..9488)
                     /gene="rsgA"
                     /locus_tag="PKL42_05885"
     CDS             complement(8577..9488)
                     /gene="rsgA"
                     /locus_tag="PKL42_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNU66642.1"
                     /translation="MHTKTPTTSIANHFIPPLQGIIVAAYGKRFQVELPDKKRISCVT
                     RGKKTDLTCGDVVTIKLTDQHEGVVETTLPRRTLLYRSNAFKSKMLAANVTQIVVVLA
                     TQPSFYEGLLNRCLIAAEAANIKPLIVLNKCDLPDHDHASEKLALYTTLGYKVLRLSA
                     KEDITALKPYLEGETSILVGQSGMGKSTMINALLPNESVRTQEVSHVLDSGKHTTTAT
                     HLYHLDSTSQLIDSPGLQEFGLHHLSAGELEHAFVEFRPYLGKCRFNNCKHLQEPDCA
                     IKAAVTSQDIHAARLAMYQTLRAELST"
BASE COUNT         2606 a         1985 c         2207 g         2814 t
ORIGIN      
        1 tttcaatcac ggggtttgca tcaccagggt caacaacaat cgcatctttg ccattgtgga
       61 ttagccagat ataattattc tcaaacgctg gaataggtat aatttcaata ttatgttgca
      121 tagtcgctat catttgtttt acacctgtaa atgtcaatag agacacggcc tgattctgat
      181 tggtttaatg gcaccgctgg gcgctatttg ctgagccaag aacaagcttg gtatgacaat
      241 gaagtatttg atttgtttgg ctttaatgcc ttgcaaatgg gctgcttgca catggactta
      301 ttgcgcaatt cgcgcattcc taaccgttat ctatccgctg attccgttac tgcaacacag
      361 ccacatcata tcagttgttg cgatgacttt ttgccttttg ctgatgccag tcttgattta
      421 ctcgtcttgc cgcatcgctt ggaattcagt gaacgccccc accaaacctt gcgcgaagcg
      481 gcaagggtga tgatgcctga aggccattta ttgatttccg gatttaatcc aacaagcact
      541 tggggcgcaa ctgcgcgaat gaacgcgtgg ttttgtaaaa ataaaactta tccttggaac
      601 ggtgaattta ttggcctgac acgcttaaaa gattggttag cactactagg ttttgaagtc
      661 atgtgggtag ataaacgctg ccatattcca ccgtttgagc aggccagctg gcataaacgt
      721 tttactttta tggacaaact gggcgcacgc tgcgggggtg tgtatttaat tgttgcaaaa
      781 aaacgtgtgg tcggcatgac gccaatcaag ccaaattgga aagcgcatcc actgaaatct
      841 gcgctggtgg caaggcgacc gaaatcaaat acttcacaaa acacgacctc acaatttgat
      901 gcaacaaaat aggtttttag cataaattaa atgcaaacct ttttcgatgc tctgcaagcc
      961 tttatttata aggctcacag ggcagtattt ttttctcgta tgattttatt gcttaataca
     1021 aataaaaaag gccagtaaaa ctgacctttt tttgtcatgt cacaactgca atttgttcta
     1081 cactaactta tcaataatcc acttactttt cacaccttaa tttggcttta atggtgtcac
     1141 ctgctttgta ttcctgcaag gtttcgacag aagcttgaaa ctcatgaatc gtgccgttgc
     1201 tatcgcgcac agtaattttt tcattggtgg tatcaacatt gatcacttta ccttctagct
     1261 gagccggttg gccagttgtg gcacaggtga cagcagcgtc ttcagcaacg gcaacgccag
     1321 tgaccacggc agaagcgatg gcagcaacca agataaattg tttaacaata tgcatgttcg
     1381 tctccattat tttgtgatgt cgtaaactgc gccgacggca gcaccaatgc ccgtgccgat
     1441 taacgcgcct tttttggcgc catggcctgt gccggcacca acggcagcgc cagcgcccgc
     1501 accaacagca gcgcctgtta cggtgccaca tgcggtagca agtaaggtta caagcgttaa
     1561 gctggtaatc actgtgagtt tggatttttt gctaatcaat tggctggtct tgttcatatt
     1621 ttgtatcctt cttttgaata aagaaaatgc aaaagtgcaa gtgcatcaac gtacttaaat
     1681 catgcttcgt tgacagtttc attctacatt caattcatca atactgaatg gctttattac
     1741 cttaaaaccg agaataacaa tttttaacaa aaataatcaa tcatggatta aaccaattgg
     1801 ctagttgggc tattacgcgt tgtgtattga atgcactata atgccgttta gtgtatgcaa
     1861 agcacaggtt tttgaatgat cataaacaga tgagtagtca agcaatcggc agcaaaacag
     1921 aagttgaaat ttacgcagat ggcgcatgta aaggcaatcc tggtccaggt ggctggggtg
     1981 cgtggattcg ttatggtagc aaagaaaaag aactgtttgg cggcgagttg cttacaacca
     2041 ataatcgcat ggaactgacg gcggtgattc gtgcgttaga ggcgttaaac cgtacttgtc
     2101 atgccaaagt gtttacagat tcagtttacg tgcaaaaagg catttctgaa tggattattg
     2161 gctggaaagc gcgcaattgg cgcactgcag ataaaaagcc tgtgaaaaat gatgatttat
     2221 ggcgcgtttt agatacgctt gcccagcaac acacagtgca atgggtatgg gtgaaagggc
     2281 acgcagggaa cgtgggcaat gaacgcgctg atatgctcgc taatctcggc gtggcacaag
     2341 tattacaaaa tgagaatgaa aaatgaggca agttttttta gatactgaaa cgacaggttt
     2401 ataccacgcg caaggtcacc gtgtgattga agtggcggca gtagaagtgg tgaatcgccg
     2461 attgactaaa caacattttc attactattt aaacccagac cgtgaaattg accaaggcgc
     2521 gcaagaagtg catggcattt cactcgagtt tttacaagat aagccacgat ttgctgatat
     2581 tgcgcatgat ttaattgcct ttattgccga tgctgaactc atcatgcata acgcaccgtt
     2641 tgatgtgggt tttttaaata tggaatttgg cctaattgac caaaagccag tagaatctat
     2701 tgcggctaag attaccgata cgcttaaaat tgccaaagaa atgcggccag ggcaacgcaa
     2761 cagcttagac gcgctgtgta agcattttgg tattgataac tctaagcgca cgttgcatgg
     2821 cgcattacta gatgccgaat tgctggcgga cgtttacatg gcgatgacgc gtggccaaga
     2881 tagcttaatg atggaattaa accagccgca aatcagtatg gaggcgcgag cattgcagca
     2941 gaatgtgccg ctgatgatta aacgtgtcag cacagaggaa gaaactgcac accaagaata
     3001 tttggcagca ctggctaaat cgggtgcttg tttgtggcaa aagttaaaag aagaaatgcc
     3061 atgacatttt gcatttagag aatgattaac attgtattga aatttaattt ttagaaagag
     3121 atgaaatgaa tcaaacgatt attgtgacag gcggcgcagg atttattggc gctaattttg
     3181 tgttggcttg ggtaaagcag ggcttaggca cggtggtgaa tttagataaa ttgacctatg
     3241 ccggcaattt agaaaatctt gttgatattc agcaaaaccc acagcatatt tttgtgcatg
     3301 gcgacattgg cgaccaagcc ttggtgtcaa aactgttggc cgaacataag ccatgcgcgg
     3361 tggtgaactt tgctgctgaa agccatgtag accgctctat tcatggccca gaagatttta
     3421 ttcaaaccaa tgtggtcggt acttttcatt tgttggaatc tgtgcgcgct tattggggcg
     3481 ctttgaatga aactgacaaa aaagcctttc gatttttgca tgtttcaacg gatgaagtct
     3541 acggctcgct cagtaaagac gaacccgctt ttgtagaaac ccgtaaatac gagccaaata
     3601 gcccatactc tgcttcaaaa gccgcttctg accacttagt gcgcgcttat caccacacct
     3661 atggcttgcc agtgctgacg accaattgct ctaacaatta cggcccgcat cattttcctg
     3721 aaaaactgat tccgctggta attcacaacg cgttagctgg aaaaccgttg cccatttacg
     3781 gcaatggcca gcaaattcgt gattggctat acgtgcaaga tcactgttct gctattcgcc
     3841 gtgtgcttga agcgggcacg gtgggcgagg tgtataacgt gggcggctgg aatgaaaaac
     3901 ccaatattga agtggtgaaa accttatgca aaatgttgga cgcgaaaaag ccccgcgcag
     3961 atggcaagcc gtatgccgac caaattacct atgttgaaga ccgtcttggt catgatcaac
     4021 gctatgcaat tgatgctact aaaattgcac gtgagttagg ttggaaaccg caagaaacgt
     4081 ttgaatccgg cattgaaaaa accgtgaatt ggtatttaga acatcaagaa tgggttaata
     4141 atgttaccag tggcgactac cgaaattggg tacaaaaaca ctacgttgac cgtggcgaag
     4201 aaatggctta atcattaagt ttagactgaa aaaattacgc taaaaaacac ctaataaagc
     4261 gtaaatgatt gcgcaacctt ttttgatgcc ctgagagcct tgtaaataaa ggctctcagg
     4321 gcatttattt tttctcgggc gaaatgcgtg gcttaactga gtgcgtctgc ccagttattg
     4381 ggggtttcat aaagacgcac tttttccagt tttaaatgat tgccgtaggt gtcttggtat
     4441 ttggttttca gaattttaaa cgcttctgcg gccatgttct ccgcagtggg gacagtgggg
     4501 aacaccaccg ttttatgttg tggaagtgaa tttaaaaaat taagcacgtc cgtgtcgtgt
     4561 tggtacacta aaaaagcatg gtcccacaca tcaactaggg cagatttggc aatggcttta
     4621 acgtcagaaa agtccatcac cataccgttt tctgaggcgt gttcgttttt gatgatgtcg
     4681 ccagaaagcg taatttcaat ggcataacga tggccatgca agtttttgca ttggctttta
     4741 tgacaaggaa tgcggtggcc ggcatcgaat tctaagcggg ttgtaatttg catgggtttt
     4801 acgtctattg cactaaagtt gagcagcaat tatacgcggt caagcgaaaa gtcatcaaaa
     4861 atttatcgtg tagaaattga aggggatgac tgagttggtg gctttaggtt gaatgtgttg
     4921 cctcaaaagc cgcaagccaa gtggcaaatg cctcaaaggg caggggttta gaataataat
     4981 agccctgaat tttgtcacat ttcatcattc taagcagctg taagcacgct gggtcctcaa
     5041 cgccttcggc gaccacttct ttacccaggc tatgtgctaa tttaagaatg gatacgacaa
     5101 tttctttatc ttgatttgat gtggcgatat ttctgacaaa agattggtca attttcacca
     5161 aatccaccgg catgttgcgt aggcgcgcca tggatgaata gcccgtgcca aagtcatcca
     5221 aggcaagcct gcagcctaga gaaacgattt gatcgagtac ttgggagact ttttcttcat
     5281 tggctaaaat gtctgtttcg gtaatttcca tcaccagatt ttctgcagga atctcccaca
     5341 acgcgatgga ttggctgagt aattcaggca aatcttcatc cagtaaatcg gtagtacaca
     5401 ggttaatggt taataagaca ttacgctgat gatttgataa caactcggcg cagtgccggc
     5461 acgcactgtt gataagccag cgaataaata ccttaccaaa gccttttttg taaatggttt
     5521 caataaggcg gaaaggtgaa accgatggcc attcttcgct aatccaacga agcagcaact
     5581 ctgcactaac acaccgctct acaggatttt ctgcattagg gagtgtaaca atcggctgaa
     5641 aatacatttc caattcgtta tgctgcaacg ccgtaataat cgcttcttct agctgatgcg
     5701 ccttttgatg catgtttgag atgactttat cgtaaatctc aatgcgcgaa tcgttgacca
     5761 acgcatggtg cagcgcgagt ttggcattgt caaacagcga gagggcgctg atgggttctt
     5821 cgagtgaaca gatgccgcca aaaattggtt ttaaaatcag ggtgacgttt tcaagtggca
     5881 attccgtttc aaacgcgtga gcaagcttag ctgccattaa atttaactgc gctgaaaaat
     5941 gtaagttttc gacaataatg gcaaactcaa acacaccaat tctaaatagc gtggcgtctg
     6001 agctcaggtg ttgctcaacc acatcaaccg ccgctcgtac gatttggttg gaaatagcgt
     6061 tgagctgtgg tgttttatca aagttgatat tgagttgaat aacgagaatg ccgatttttt
     6121 ctgaagcaag cacatcgttc gcttgcgcta ctgcagcctc agttactggt gaatagcggt
     6181 taagaatatc cagcaaaaat cgctgattgg gtaaccctgt ttctaaatca tagtgttgaa
     6241 tgtagttaaa ctgaaacgca tcaaattggt gggtggccgt tgccagcaag tgcatcaaaa
     6301 aactttgaaa ttttaagctg acggtttggt cagcgtgttg caaatcttgc gtgcctatgg
     6361 cttctgcagt ctgtagcgtt aagctgcgag ctaatacaaa tgcattggct aatgctgcgc
     6421 cagcttggta atcttccagc catttttgta ataagcgatt gatcattgtg ttaaattgca
     6481 cccaatttaa ctgtttaggc cattggcgaa taacgtcaat ggatttttca tggtttgcct
     6541 ctgctgcatc catcgtgtta aacggcggca gactgcgtag tgggtgaaat agcgcgctac
     6601 ttacttgata aagccccaca acatctgagg ctttcagacc ataagtgttc atcagttgca
     6661 caagttcatt gggtgacaat gcaaaagaaa acgccggtgc tgtagccact tggcttggtt
     6721 ttgcctcggt cagtgctaag ctactttgat tagccataga aattgctggg gttgagtccg
     6781 taactgtcgg cgggtaaccc acgcacgatt ttatagggct cgccttttgg ggtgagagga
     6841 gatgtgatta agttaatggt tgccggatat tcatttttag ttttatccgg gttgagtaaa
     6901 atcatcactc tcggcaacag acggcgcaca ctgttttgct gaatcaccac gccgacttca
     6961 cctgagttaa gttctaccag tgaactcaca gggtacatgc ctaaaaactg caccaattgg
     7021 ttgaccaatg ccgcactaaa tttaacatca ctcattttgt ttatgcgttc tagcgctact
     7081 tggttataca cccctttggc atacacctta ttggtggtga gcgcacagta ggtatcaatt
     7141 aagcctgcca tttggccgcc acggttgatt tgtttgccag aaagtgcatt cggataaccg
     7201 ctgccgtcaa aacgctcgtg gtgttctgcc acaatcgcaa tgacactttc aggaatatct
     7261 tcggtttctt ttaatatgtc aatggcataa tccacgtggc gtttcagcac cgaaaattcg
     7321 gcactggaga tttttccctc tttgtgcagt agttgtgctg gcaacttacc tttaccaata
     7381 tcctgcaata agcctgccat gccgagattt ttaatttgcg ctttaggaaa cgacataaaa
     7441 ctggcaaacg ccatgagcgt gatggataca ttcagcgcgt ggttataggc gtagttgtcg
     7501 gttagtttga gtttggcaag ccacatcagc gcatctgggt tacgttcaat actatccacc
     7561 atgccatcca gcgcttcatt tacattggtg atgtcaattt gctgcccggc cgccagtgcc
     7621 tcaaaaatct cacgcgtggc gatttgagat ttatcatagg ttggatagat ttcagcaatt
     7681 tcatcttcaa ccagtgaagc ttcttcaatg attgtgactt gatctaggct attattttcg
     7741 tcagcatttt cgccattatt tttatcagtt ttatctttaa gttttttatt tttgctaccc
     7801 cagctggtaa gtccaccgat gaagctcgta aagtcttctt taattttact tgctaggctt
     7861 gggttttctt caggctcatc tttttcaatg atgggcgtat agatttgatt gttgatattt
     7921 tgcacattca gcagaatatt gtcggtagtt ttagcgccgg ggttattcgc ctgattcgca
     7981 aggctggctt gattagccgc ctgtatttct ttcataacgt taaaaaacgt aaatttttga
     8041 ctgctggggc tgcgcttggc taatttagtg ccagataaac ctgattttgt atttgcactg
     8101 atgggcattt tggaagtgat gccatcgcgt ttaatgccaa tttcttcttt gggtggggca
     8161 ataaaatgtt tgccaatcga catcgtatgg tcaacataaa ccactttgca tagctttgcc
     8221 atagcggcaa tttgttcttg ctcttctagc agcaggcctt ccagcataaa gggcgtgcct
     8281 agccaaggcc ggtcaagggc tgtaacaaac atgcccattt ctaattgtga aaccggggtt
     8341 tcttttcgac tcacgtcact catttcactc aaaaatacct ttttgctttt atttgttaat
     8401 tcgtttaaag accaatgaat caatattatc ttattttgat tacggtatta cattgcgata
     8461 ttaaccagtt tctaattatt gcagttgata aatatcaaac caataattga ggcgtggtta
     8521 ggtgcaagcg tcccaaatca ttcttcaacc ataaagcaat agttatgcca aaaaatttaa
     8581 gtagatagct ctgcacgcag cgtttgatac attgccaaac gcgctgcatg tatatcttgc
     8641 gaggtaactg ccgctttaat tgcacagtct ggctcttgca ggtgcttgca attattgaat
     8701 ctgcacttgc ctaaataggg tctaaactca acaaaagcat gttccagttc accagcactt
     8761 aaatgatgca ggccaaattc ttgtaagcct ggggaatcaa tcaactggct tgtgctgtcg
     8821 aggtgatata aatgggtggc ggtggtggta tgtttgccgc tgtctaacac atggctgact
     8881 tcttgggtgc gtacgctttc gttgggtagc agggcgttaa tcatggtaga tttccccata
     8941 cctgattggc caacgagaat gctggtttca ccttctaaat aaggtttgag tgctgtaatg
     9001 tcttcttttg cggaaagacg caacacctta tagccaagcg ttgtatagag ggccagcttt
     9061 tcacttgcat ggtcatggtc aggcaagtca cacttgttta acacaatgag tggtttgata
     9121 ttggcggctt cggctgcaat taaacagcgg ttgagcaagc cttcataaaa gcttggttgt
     9181 gtggcaagca ctacaacgat ttgcgtcaca ttggctgcca gcattttgct tttaaaggcg
     9241 ttgcttcgat agagcagcgt tctacgcggc agggtcgttt caaccacgcc ttcatgttga
     9301 tcggttaact taatcgtcac cacatcacca caggttaaat ccgttttttt gccacgggtc
     9361 acgcagctaa tgcgcttttt atcaggtagt tcaacttgaa atcgcttgcc gtaagccgca
     9421 acgattatgc cttgcaaagg cggaataaag tggttggcaa tactggttgt tggcgttttc
     9481 gtgtgcaaat tattcagtca atattttggc taagattatt gataacgtgt acatttttga
     9541 tataaaaaag cccttaaaga tcaccgagaa aaaataaatg ctctgaaagc ctttatttac
     9601 aaggctcgca ga
//
LOCUS       DAOQSI010000071         9585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_6506_length_9585_cov_5.879958, whole genome shotgun sequence.
ACCESSION   DAOQSI010000071 DAOQSI010000000
VERSION     DAOQSI010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 9585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9585
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6506_length_9585_cov_5.879958"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PKL42_05890"
     CDS             complement(<1..326)
                     /locus_tag="PKL42_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNU66643.1"
                     /translation="MSNPTAKVQLPAIPAKRYFTIGEVSELCGVKPHVLRYWEQEFTQ
                     LKPVKRRGNRRYYQHHEVLLIRRIRELLYEQGFTISGARLRLDEANGSESNVNTVQLS
                     STLAEH"
     gene            complement(373..666)
                     /locus_tag="PKL42_05895"
     CDS             complement(373..666)
                     /locus_tag="PKL42_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HNU66644.1"
                     /translation="MTLTKAELADLLYEQVGLNKREAKDMVEAFFEEVRIALEAGDSV
                     KLSGFGNFELRKKSERPGRNPKTGEEIPITARRVVTFHASQKLKSKVEEHYAK"
     gene            complement(780..3134)
                     /gene="pheT"
                     /locus_tag="PKL42_05900"
     CDS             complement(780..3134)
                     /gene="pheT"
                     /locus_tag="PKL42_05900"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNU66645.1"
                     /translation="MQFSENWLRTLVDTDLDSQALSHALTMAGLEVEDMQPVAASFSN
                     VVVAKIVSAEKHPDADRLQVLKVDIGQGEPLQIVCGAANARVGLIAPCALVGAELPGI
                     SIKQAKVRGVESFGMMCSSKELGISAEASGLLELESHAVIGLNIREHLDLDDHVFTLK
                     LTPNRSDCLSITGIARDVAAITGAKTKFVEITPATATLQQTKQVTVNAQAVCPRYCGR
                     LISGVNANAPTPVWMVKRLERSGLRSISAVVDVTNYVLLELGQPLHAFDAAKLNGDIS
                     VRFANAGESLNLLNDQTVELKVDDLVIADASGPVALAGIMGGKPTSVDDATMELFLES
                     AFFIPAVIAGKARRIGLSTDSSYRFERGVDFGNTRNAIEYATTLIQQICGGSAGKITE
                     VAGEMPARHAVKLRMTRLVSVLGIAFEQEKVAQLLTQLGFSYKELNNVFTVTPPSYRF
                     DIAIEEDLIEEIARLHGYDHIPATPPVATLRMLASPEKELALNQLRDALVAEGYQEVV
                     TYSFVDESWERDLLGNLNPIKLKNPIASNLSVMRTSLWGGLLDALRYNLNRKQDRAFV
                     FEIGAVYHQQGVSYTEATRISGLAYGSALPEQWATTGAEVDFFDVKAHIEGLLGSKAV
                     FEKAEHTALHPGQSAKILLNGKVVGRFGKLHPKWQQQYDLPNSAYLFEIEVAAIVSRD
                     LPSYHDVSKLLPVRRDIAIVIDENIAAGDVLSCINNANLLLVSEVALFDLYQGKGVAE
                     NKKSLALSVLMHDTQKTLTDNDADTTMANLLQLLQNEFKAELRN"
     gene            complement(3269..4267)
                     /gene="pheS"
                     /locus_tag="PKL42_05905"
     CDS             complement(3269..4267)
                     /gene="pheS"
                     /locus_tag="PKL42_05905"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNU66646.1"
                     /translation="MADLTHLISEAQTDFACSTTMNDLEVAKAKYLGKSGLLTDALKG
                     LGKLTAEERPAAGAAINVVKQAVEAALTARREALLNAEQAKQLAQESIDVTLPARAQA
                     QGGLHPVTLTLKRVESLFHSIGFEVATGPEIETDFYNFTALNIPEDHPARAMHDTFYI
                     DDAHVLRTHTSPVQVRYMENALKINKTPPLKIIAPGRVYRVDSDATHSPMFHQVEGLW
                     VDEDISFANLKGVVQDFLQKFFERDDLTVRFRPSFFPFTEPSAEMDMSWNGGWLEIGG
                     CGMVHPEVFKHVNIDSEKYRGFAFGLGIERLTMLRYGVNDLRHFFNNDLRFLSQFR"
     gene            complement(4368..4727)
                     /gene="rplT"
                     /locus_tag="PKL42_05910"
     CDS             complement(4368..4727)
                     /gene="rplT"
                     /locus_tag="PKL42_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNU66647.1"
                     /translation="MPRVKRGVIARARHKKVLKAAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQKKRQFRTLWIARINAGARELGLTYSRFMNGLKKASIEVDRKVLADLAVFD
                     KAAFAKFVESAKNALAA"
     gene            complement(4738..4935)
                     /gene="rpmI"
                     /locus_tag="PKL42_05915"
     CDS             complement(4738..4935)
                     /gene="rpmI"
                     /locus_tag="PKL42_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008107656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNU66648.1"
                     /translation="MPKMKTKSSAKKRFKFLGNGKVKRTHSHLRHILTKKTTKQKRKL
                     RGTSIISPTDINRVRAMMPTR"
     gene            complement(5072..5596)
                     /gene="infC"
                     /locus_tag="PKL42_05920"
     CDS             complement(5072..5596)
                     /gene="infC"
                     /locus_tag="PKL42_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNU66649.1"
                     /translation="MAQDKETRINGDITASPVRLLGIEGEPLGIVALAEAFAKAEEAD
                     IDLVEIAPNASPPVCRLMDYGKFKYAESKKQHEVKLKQKQVQVKEIKFRPGTDDGDYS
                     IKVRNIIRFIEDGDKAKITLRFRGREITHQEFGLALLRRVEADTKEVAVVEQFPKMEG
                     RQMVMVLGPHKKAK"
     gene            complement(5654..7561)
                     /gene="thrS"
                     /locus_tag="PKL42_05925"
     CDS             complement(5654..7561)
                     /gene="thrS"
                     /locus_tag="PKL42_05925"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNU66650.1"
                     /translation="MPVIRLPDGSQRSFDAPVTVAEVALNIGAGLAKAALAGKVNDKI
                     VDTSFLIEADSDVAIITDKNPEGLDIIRHSTAHLLAYAVKELFPDAQVTIGPVIENGF
                     FYDFSYVRAFTPEDLLMIEKKMVELAKKDEPVTRQVLPRDEAIAYFKSIGEDYKAEII
                     GAIPAGEEVSLYTEGRFTDLCRGPHVPNTGKLKAFKLMKLAGAYWRGDSNNEMLQRVY
                     GTAWRNKEELDAYLFQLEEAEKRDHRKLGKQLDYFHMQDDAPGMVFWHPRGWSIWQEI
                     EQYMRQMFRDFGYQEVRTPTIMDKTLWEKSGHWQNYRDNMFVTASESRDYAVKPMNCP
                     GHIQIFNSGLHSYRDLPLRLAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQI
                     ESEVADFIQMLYKAYGDFGFTDVLVKLSTRPEKRVGSDETWDKAEASLANALNKNNLA
                     FDLQPGEGAFYGPKIEFTLKDSLGRLWQCGTIQLDFNLPERLDAEYVAEDNSRKRPVM
                     LHRAIVGSMERFIGILIENYAGAMPLWLAPVQAMVLNISENQADYVRQVVENLKKNGI
                     RCEFDLRNEKITYKIREHSMQKMPYLLVAGEREMQTGHVAVRTRKGEDLGSMPIAAFI
                     ERLKAEVETKA"
     gene            complement(7876..8715)
                     /locus_tag="PKL42_05930"
     CDS             complement(7876..8715)
                     /locus_tag="PKL42_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNU66651.1"
                     /translation="MATILIIGCGDLGSAVALKLAQLGHRVVGVRRSATPLTNVEMIQ
                     ADVTHPETLDKLAKIQPDILVYCVAANGQTDAHYKAAYVDGLRNVIATQVNNTALKQV
                     FFVSSTRVYGQVTDAVLDENTPAIAADFGGERLLEAERLLEGLTCGHTALRLSGIYGP
                     GRLRMIHLAKTPALWPAQNSWTNRIHRNDAAAFLVFLVQKVLHQTPIAPCYIVTDAAP
                     VSQYEVLQWLALQHNIAFNAHVPAIQSGKRLSNRATLATGFQLQYPDYRAGYAALLAL
                     NAD"
     gene            complement(8720..>9585)
                     /locus_tag="PKL42_05935"
     CDS             complement(8720..>9585)
                     /locus_tag="PKL42_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HNU66652.1"
                     /translation="ERPARMQAIEQALQRAGLFSKLAQYEAPLASKAVLARAHTNAYI
                     DWIFSQSPKTGLVQLDADTAMNAHSLEATLRASGAAVKGVDLVMTGQMQVAFCNTRPP
                     GHHAGRASSAGFCIFNHVAAAALHAIAQYGLTRVAIADFDVHHGNGTEDIFHDDPRVM
                     LCSSFRHPYYPYSGADSGNDHIINTPLAAGSDGKALRKVVNEQWLPALARFKPQLLII
                     SAGFDAHQEDSMGGLKWLEEDYVWVTEALKAVAKQHAEGRIVSVLEGGYALDALGRSA
                     AAHLNTLIAAS"
BASE COUNT         2476 a         2355 c         1999 g         2755 t
ORIGIN      
        1 tgatgctcag cgagagtcga tgacagttgc actgtattca cgtttgattc gctaccatta
       61 gcctcatcta aacgcagtct tgcaccactg attgtaaagc cttgctcata caatagttcg
      121 cgaatacggc gtattaataa aacctcatgg tgctgataat aacgacgatt gccacgtctt
      181 ttaacgggtt taagctgagt aaactcttgc tcccagtacc taagtacatg aggtttaaca
      241 ccacaaagct cactcacttc accaatggta aaataccgtt ttgctggtat cgctggtaac
      301 tgcacttttg ctgttggatt actcataggc gctcacttaa cttattgttt aggatgctaa
      361 ataaattaat ttctattttg cgtaatgctc ttcaacttta ctttttagct tttgacttgc
      421 atgaaaagtc acaacgcgac gtgcagtaat tggaatctct tcacctgttt tagggtttcg
      481 gccaggacgc tctgactttt tacgcaactc aaaattacca aagccagata gctttacgct
      541 gtcacctgcc tcaagcgcaa tacgcacttc ttcaaaaaag gcttccacca tatcttttgc
      601 ttcgcgctta tttaagccca cttgctcata aagcaagtct gcaagttcag cttttgttaa
      661 tgtcatatta cactcccgct tgtttctagc attttaatgc tttttttatg taccaataat
      721 caattaaaaa ctgatgaaaa atgaaaaatc agtgaattta atataaaaaa taggtaccct
      781 taatttctta attccgcctt aaattcattt tgtaacaatt gtaacagatt tgccatcgtt
      841 gtatcggcat cattatcagt taaagttttt tgagtatcat gcataagtac tgataatgca
      901 aggctttttt tgttttctgc gacacctttg ccttggtata aatcaaataa cgccacctct
      961 gatacgagca gtaaattggc gttattgata caacttaata cgtctccagc agcaatattt
     1021 tcatctatca caatggcaat atctcggcgt acaggcagca attttgacac gtcatgataa
     1081 ctgggcaagt cacggcttac gattgcagcg acttcaattt caaataaata ggcactattt
     1141 ggtaagtcat attgttgctg ccatttcggg tgcaatttac caaaccgacc gactaccttg
     1201 ccattgagca atattttggc agattgtccc gggtgcaatg ctgtgtgttc tgctttttca
     1261 aacacagcct tagacccaag caacccttca atgtgtgctt taacatcaaa gaaatccacc
     1321 tccgcacctg ttgtagccca ttgctctgga agagcacttc cgtaggccaa acctgaaatt
     1381 cttgtcgcct ctgtataact caccccttgt tggtggtata cggcgccaat ttcaaaaaca
     1441 aatgcccggt cttgtttacg atttaagtta taacgcagag catcaagtaa cccaccccat
     1501 aaacttgtac gcatcacact taaattactg gcaatcggat tttttagctt gatggggttc
     1561 agatttccta ataaatcacg ctcccagctt tcgtctacaa agctgtaagt cactacttct
     1621 tggtaccctt ctgctaccaa cgcatctctt aattggttaa gcgctagttc cttttcaggg
     1681 cttgccaaca tgcgcaacgt tgccactgga ggagtagctg gaatatggtc gtaaccatgt
     1741 agccgtgcaa tttcttctat taagtcttct tctattgcaa tatcaaaacg gtaacttggt
     1801 ggtgttaccg taaaaacatt gtttaactct ttataagaaa atcctagttg cgttaatagc
     1861 tgtgctactt tttcctgctc aaatgcaata cctaacactg aaactaaacg tgtcatcctt
     1921 aatttaacag catgacgcgc aggcatttca cccgcaactt cagtgatttt ccctgcgcta
     1981 ccgccacaaa tttgctgaat caatgttgtc gcatattcaa tggcattgcg cgtgttacca
     2041 aaatctacgc cgcgctcaaa ccgataagat gagtctgtgc tcaagccgat tcgtcgcgct
     2101 ttacctgcaa ttaccgccgg aataaaaaag gcactttcta aaaatagttc catcgttgca
     2161 tcgtctacgg aggtaggttt tcctcccata atgccggcta acgccactgg tccactagca
     2221 tcggcaatca ccaagtcatc tacttttagt tcaacggttt gatcattgag taaattcagt
     2281 gactcacccg catttgcaaa acgcaccgat atatcaccat ttaatttggc cgcatcaaac
     2341 gcgtgtaaag gctgacctaa ttcaagtaac acatagttgg tcacatcgac cacagcacta
     2401 atgctacgca aaccgctacg ctcaagacgc tttaccatcc acaccggcgt tggggcattt
     2461 gcattaaccc ctgaaatcaa acggccacaa tagcgtggac ataccgcttg cgcattcacc
     2521 gttacttgct tggtttgttg caacgtagcc gttgcaggtg taatttcaac aaatttagtc
     2581 ttggctccag taatggcagc cacatcgcgc gcaataccgg taatactcaa gcaatcactg
     2641 cgatttggtg tgagttttaa cgtgaataca tggtcatcta agtccaagtg ctctcgaata
     2701 tttaggccga tcacggcatg gctctccagt tctagtaagc ctgacgcctc agcgctgatg
     2761 cctaactctt ttgatgaaca catcatgccg aaagactcta caccacgtac tttagcttgt
     2821 ttaatcgaaa tgccgggcaa ctcagcacct accagcgcac atggggcaat tagtccgacg
     2881 cgtgcattgg ctgcaccaca cacaatttgc agaggttctc cttgaccgat atccacctta
     2941 agaacttgta ggcggtctgc atctggatgc ttttcggctg atacgatttt agcaaccacg
     3001 acattgctaa aagatgcagc aacaggttgc atgtcttcaa cttctagccc tgccattgtc
     3061 agggcgtgac ttaacgcctg actgtccaag tctgtatcca ctaaagttcg taaccagttt
     3121 tctgaaaatt gcataatgct ttaatgcctt caattataaa aatgcactcg ctaaaaaatc
     3181 ttttagccgc agataaacgc agatgtacgc aggttttgaa ttaaaactaa agtatttagc
     3241 tattatctgc ggctaacaaa ataatctatt acctgaattg gcttaaaaag cgcaaatcat
     3301 tattaaagaa atggcgcaaa tcattcacgc cataacgcaa catggttaaa cgctcaatac
     3361 ctaaaccaaa ggcaaagcca cgatatttct cgctgtcgat attcacatgt ttaaacactt
     3421 ctgggtgcac catgccacaa ccaccaattt ccagccaacc accattccag ctcatatcca
     3481 tttcagcaga gggctctgtg aaaggaaaaa atgagggtct gaaccgcaca gtcaagtcat
     3541 cgcgctcaaa gaatttttgc aggaaatctt gcacaacgcc ttttaaattg gcaaaactga
     3601 tgtcttcatc cacccacaga ccttcaacct ggtgaaacat cggcgagtgc gttgcatctg
     3661 aatccacccg gtaaacacgg cctggcgcaa tgatttttaa agggggtgtt ttgttgattt
     3721 ttaaggcatt ctccatatat cgcacttgca caggcgaggt atgcgttctg agcacgtgcg
     3781 catcatcaat gtagaacgtg tcgtgcattg cgcgcgctgg gtgatcttca ggaatattca
     3841 aggcagtgaa attataaaaa tctgtttcaa tttcagggcc ggttgccact tcaaaaccaa
     3901 tggaatgaaa tagcgattca acacgtttta gcgtgagcgt gactggatgc aaaccacctt
     3961 gagcctgtgc acgggcaggt aaagtcacat caatcgattc ttgcgctagc tgtttggcct
     4021 gctctgcgtt taacaaagct tcacgacgag cagttaaagc ggcttcaact gcttgcttga
     4081 ccacgttgat ggcagcaccg gcggctggac gctcttcagc agttaacttt cccaagccct
     4141 tcaaggcatc cgtcagcagg ccagattttc ccaaatactt tgctttggca acttccaggt
     4201 cgttcatggt tgtgctgcaa gcaaaatctg tttgcgcttc tgaaattaaa tgcgttaaat
     4261 cagccatata ttttaagaat ctttaaatta actgcggagg aatgacacaa tttattgatt
     4321 ttacagtaaa aaaaagaggc ctaagcctct ttttttgttt tttcaactta agcggctaaa
     4381 gcattttttg ctgattctac aaatttagca aatgctgctt tatcaaatac cgccaagtca
     4441 gccaagactt tacggtccac ttcaattgaa gcttttttca agccgttcat gaagcggctg
     4501 taagttaagc ctaactcacg tgcgcctgca ttaatacgtg caatccacaa ggtgcggaat
     4561 tgacgtttct tttgacggcg gtcgcggtat gcatattgac cagccttcat caccgcttgc
     4621 ttggcaacgc ggtaaacgtt tttacgacga ccgcgataac cttttgcggc ttttaatact
     4681 tttttgtgtc ttgcgcgtgc gatgacacca cgttttactc taggcatatc ggtctcctta
     4741 tcgtgttggc atcattgcgc gaacgcgatt gatgtctgtt ggtgagatga tcgacgtacc
     4801 acgtaattta cgtttttgtt ttgtcgtctt ttttgtgagg atatggcgta aatgtgaatg
     4861 tgtacgtttc actttaccat ttcccaagaa tttgaagcgt tttttggcgc tgctcttggt
     4921 tttcattttt ggcattttgt tctccttgaa cttgagttat gagtgcttag gcatgccgtt
     4981 tagccgtatc actcggaatc ttcgctacta cttttttgct acttttaatg caattacatt
     5041 aaatcttgtt actttattgc ttatgacttg gttacttcgc ttttttgtgc gggcccagca
     5101 ccatcaccat ttggcggcct tccattttag gaaactgctc gaccactgcc acttcttttg
     5161 tatctgcttc tacacgtctt aataaggcca gaccaaattc ttggtgtgta atctcacggc
     5221 ccctaaagcg taaagtaatt tttgctttat cgccgtcttc aataaaacgg atgatattac
     5281 gcactttaat ggagtaatcg ccatcgtcag tacctgggcg aaacttaatt tctttgacct
     5341 gcacttgttt ttgcttaagc ttaacttcgt gctgtttctt actttcggca tatttaaatt
     5401 tgccgtaatc catcaatcta cacacgggcg gactggcatt tggcgcaatt tccaccaagt
     5461 caatatcggc ttcttcagct tttgcaaacg cttctgctaa tgcaacaatc cctagaggtt
     5521 caccctctat gcccaacaaa cgaacaggtg atgcagtaat atcaccattg attcgcgttt
     5581 ctttgtcctg tgctatatca atttctccaa atagttaagc cgcgcttcga cagaaaatcc
     5641 ctgaattggg atgctaagcc ttagtttcaa cctctgcttt taagcgctct atgaacgctg
     5701 caatcggcat cgatcctaag tcttcgcctt ttctggtacg cacggctaca tgtcctgttt
     5761 gcatttcacg ctcacctgct accagcaagt aaggcatttt ctgcattgaa tgttcgcgta
     5821 ttttataggt aatcttctca tttctcaagt caaattcgca tctaatgcca tttttcttta
     5881 aattttcaac tacttggcgc acgtaatcag cttggttttc tgaaatattg agcaccatcg
     5941 cttgtacagg tgccagccag agcggcatcg cgcctgcgta gttttcaatg agaatgccaa
     6001 taaagcgctc catcgagccg actatcgcac ggtgtagcat cactggacgc ttgcgtgaat
     6061 tgtcttctgc cacgtattca gcatccaaac gctcagggag gttaaagtca agctgaatag
     6121 tgccgcattg ccacaggcga cccagcgaat ctttcagggt aaattcaatt ttagggccat
     6181 aaaatgcgcc ctctcccggc tgtaaatcaa aagctagatt atttttatta agcgcgttcg
     6241 cgagtgatgc ttcggcttta tcccaagttt cgtcacttcc tacgcgtttt tctgggcgcg
     6301 ttgagagttt caccagcaca tcggtaaagc caaagtcgcc ataagctttg tagagcattt
     6361 gaataaaatc cgcaacttct gactcgattt gttcttctgt acagaaaata tgcgcgtcat
     6421 cttgggtaaa gccgcgcacg cgcatcaagc catgtaggga cccggatggc tcgttacggt
     6481 ggcaagagcc aaactcggcc aaacgcaacg gcaaatcgcg atagctatgc agcccgctgt
     6541 taaagatttg aatgtggccg gggcagttca ttggcttcac tgcgtaatcg cggctttcag
     6601 atgcggttac aaacatgttg tcgcgatagt tttgccaatg gcctgatttt tcccacaagg
     6661 ttttatccat aatggttggc gtgcggacct cttggtagcc gaaatctcga aacatttggc
     6721 gcatgtattg ctcaatttcc tgccaaatac tccagccgcg cggatgccag aacaccatgc
     6781 ctggcgcgtc gtcttgcatg tggaaataat ccaactgttt gcccagtttt cggtggtcac
     6841 gcttttcagc ttcttctagc tgaaataagt acgcgtccaa ctcttcttta ttgcgccagg
     6901 cggtgccgta tacgcgctgc agcatttcgt tattgctatc gccgcgccag taagcgcccg
     6961 ctaacttcat gagtttaaat gcttttagct tgccagtgtt cggcacgtgc gggccacgac
     7021 aaaggtcagt aaagcggcct tcagtgtaga gtgatacctc ttcacctgct ggaatcgcgc
     7081 caataatttc ggccttataa tcttcaccta tgcttttaaa gtaggcaatt gcttcatcgc
     7141 gcggcaatac ttggcgggtc actggctcgt cttttttcgc cagttccacc attttctttt
     7201 ctatcatcag caaatcttct ggcgtaaagg cgcgcacgta agaaaaatca tagaaaaaac
     7261 cattttcaat cacagggcca atggtgactt gcgcatcagg aaacaattct ttcaccgcat
     7321 aagctaacaa atgcgcggtg gaatggcgaa taatatctag cccttctgga tttttgtcgg
     7381 tgataattgc cacatcgctg tcagcctcga ttaaaaagct ggtatccacg attttatcgt
     7441 tcaccttgcc ggcaagcgcc gcttttgcaa gccctgcgcc aatattgagg gcgacttcag
     7501 ccacagtcac aggcgcatca aaactacgtt gagagccatc aggtaaacga attacaggca
     7561 tgcgatttcc aatttattaa gttgcaatta tcgttagggg attttgtcac taatatgcca
     7621 cgtggcattt gctaaaaatt caacatgaga ttatcgcata cctcattggt agtcagcaat
     7681 ttttgggaac ttaagtgtgg ttaagttgcg atgatttaag gcaagtttta cgtacgctta
     7741 atcacagacc aaaaggcata aaattgacaa aaaaccgccc gagaaaaaat ttatgccctg
     7801 caagccttta tttataaggc tcgcagagca tcgaaaaagg ttgcacgggt tttgagcact
     7861 taaaacccta aaaaattagt ccgcattaag cgctaataat gcagcataac cagcccgata
     7921 atcaggatat tgcagttgga accctgtggc caatgtggca cgattactta agcgcttgcc
     7981 gctttgtatg gctggaacat gcgcattaaa cgcaatatta tgttgtagcg cgagccattg
     8041 cagcacttca tactggctga cgggcgcggc gtccgtaaca atataacaag gcgcgatggg
     8101 cgtttgatgc agcacttttt gcaccaaaaa aaccaaaaac gcagcggcat cgttgcgatg
     8161 aatgcgattg gtccagctat tttgcgcggg ccacagcgct ggcgttttgg caaggtgaat
     8221 catgcgcaag cggcccggcc cgtaaatgcc tgaaagccgc aatgccgtgt ggccgcaggt
     8281 caaaccttcg agaagccgtt cagcttccag cagccgttcg ccaccaaaat ctgccgcaat
     8341 cgctggcgtg ttttcatcaa gcaccgcgtc tgttacttga ccatacacgc gcgtgctaga
     8401 aacaaaaaac acctgtttta aggcggtgtt attgacttgc gtggcaatca cattgcgcaa
     8461 gccatccaca taggcagctt tgtagtgggc atcggtttgc ccgtttgcgg ctacgcaata
     8521 aacaagaatg tcaggctgaa tttttgccag cttatcgagc gtttcagggt gtgtaacatc
     8581 ggcctgaatc atttcaacat ttgtaagcgg tgtggcactg cgccgcacac ccacaacccg
     8641 atgccccaat tgcgctagtt ttagcgccac ggcgctgcct aaatcgccac aaccaataat
     8701 caatatcgtt gccatataat tagctagccg caatcaacgt gttgagatgc gccgccgcgc
     8761 tacgcccaag cgcatctaag gcgtagccgc cttctaacac cgagacaatg cggccttcgg
     8821 catgctgttt ggcaacggct tttagtgctt cggtcaccca aacgtaatcc tcttcaagcc
     8881 atttcaagcc gcccatgcta tcttcttgat gcgcatcaaa accggctgaa ataatcagta
     8941 attgtggttt gaatcgcgct aatgccggca gccattgttc attcaccact ttgcgtaaag
     9001 ctttgccatc actgcctgcg gctaatggcg tattgatgat atgatcgttg ccgctgtcag
     9061 ctccactata agggtaatat ggatggcgaa agcttgaaca caacatcacc cgcgggtcgt
     9121 catgaaaaat atcttcggta ccattgccgt ggtgtacatc aaaatctgca atcgccacgc
     9181 gggttagccc gtattgtgca atggcatgca gcgccgcagc ggccacatgg ttgaagatgc
     9241 aaaagccagc tgaactcgcg cgcccagcgt gatggcctgg cgggcgcgta ttgcaaaatg
     9301 ccacctgcat ttgcccggtc atgactaaat ccacgccttt cacagcagcc cctgacgcac
     9361 gtaaggtggc ttccaaacta tgcgcattca tggcggtatc ggcatctaac tgcactaatc
     9421 ctgttttggg cgattggcta aaaatccaat caatataggc attggtatgc gcccgcgcca
     9481 gcacggcttt actggcaagt ggtgcttcat attgcgcgag tttactgaat aagcctgcgc
     9541 gctgtaacgc ctgctcaatg gcctgcatac gcgctgggcg ctctg
//
LOCUS       DAOQSI010000072         9546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_6541_length_9546_cov_6.010536, whole genome shotgun sequence.
ACCESSION   DAOQSI010000072 DAOQSI010000000
VERSION     DAOQSI010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 9546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9546
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6541_length_9546_cov_6.010536"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PKL42_05940"
     CDS             complement(<1..243)
                     /locus_tag="PKL42_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNU66653.1"
                     /translation="MMRTHYCGHLNREHIGQTVTLCGWAHRRRDHGGVIFIDLRDREG
                     MAQIVIDPDTPQAFANAETVRNEFVLKVTCKVRARPE"
     gene            complement(339..956)
                     /locus_tag="PKL42_05945"
     CDS             complement(339..956)
                     /locus_tag="PKL42_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HNU66654.1"
                     /translation="MKQYFITGLLVLVPLVITVWVLKTFIGVMDQSLLLLPDAWHPHN
                     LFKRDIPGLGAILTVAIVLVTGVLATNFFGKQLITLWENLLNRVPVVKSIYAGVKQVS
                     DTLFSDSGNAFRHAVLVQFPHSGAWTIAFITGQPGGEVAIHLRGEHVSVYVPTTPNPT
                     SGYFLMIPKEDVITLEMSVDEALKYIISMGVISPSDLVAAKKLKG"
     gene            complement(974..1258)
                     /locus_tag="PKL42_05950"
     CDS             complement(974..1258)
                     /locus_tag="PKL42_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNU66655.1"
                     /translation="MPIYDYQCSSCGHKAEVMRKISEPNLTVCPACGSETFAKQVSAP
                     SFQLTGSGWYATDFKGGQKPAASSTQTKPAESKPAEATPPAACGTGCACH"
     gene            complement(1449..1952)
                     /locus_tag="PKL42_05955"
     CDS             complement(1449..1952)
                     /locus_tag="PKL42_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNU66656.1"
                     /translation="MASVNKVILVGNLGRDPEVRFMPNGEAVCNFSIATTDNWKDKSG
                     AKQERTEWHNIVMYRKLAEIAGEYLKKGRPVYIEGRLQTRKWQTKEGQDRYTTEIIAD
                     QMQMLGGRDGGSNASYDGGMDQSGGDDYNQTAPSQGQPRQASSAQKAPAQTGGNAFDD
                     FEDDIPF"
     gene            complement(1955..3355)
                     /locus_tag="PKL42_05960"
     CDS             complement(1955..3355)
                     /locus_tag="PKL42_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNU66657.1"
                     /translation="MAISEKMTPLEVRATTSLASIYGLRMFGMFSILPIFAIYAASFP
                     EQPTPFMIGLALGAYGLTQALFQLPFGMASDKYGRKPMIYLGLGIFAVGSMVAALAMN
                     IEGVIIGRALQGAGAVSAVVTALLADLTRDEHRTKAMATIGATIGVAFAISLVGGPLL
                     NQWIGVPGIFFMTALLTLAAMAVVKFVVPDPVHSYHHSDASATPAKLKDVLKNKQLLR
                     LNYGIFALHAAQMAMFVVVPFAITKSSQIEVNQHWHIYLPVLIGSFVLMVPAIIYAEK
                     QAKMKQVFLLAIGLMLIAQLAFSAFIQYFWGIVFSLTLYFVAFNILEATLPSIISKIA
                     PAAAKGTAMGVYNTSQSIGIFVGGALGGYLSHQFGYASVFIFCSVMMILWLIFAFSMQ
                     APLAVKTKMFSMDESALNMPAAEAEALKQSLTNLHGVLEVAVLPQELTVILKIDKSKN
                     VNEAEIETLVHQLLKG"
     gene            3534..6347
                     /gene="uvrA"
                     /locus_tag="PKL42_05965"
     CDS             3534..6347
                     /gene="uvrA"
                     /locus_tag="PKL42_05965"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNU66658.1"
                     /translation="MEFIKIRGARTHNLKNVSLDIPRNQLVVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLARMDKPDVDLIEGLSPAISIEQKSTSHNPRSTVGTVT
                     EIHDYLRLLYARAGDPECPDHGIKLEAQTVSQMVDSVLKLPEDTKLMILAPVVANRKG
                     EQVDLFEELKAQGFVRLRIDGKIYEVDALPQLAKTSKHNVDVVVDRLKVKADMKQRIA
                     ESFETALRLADGKAIAVEMDSDIEHLFSAKFSCPVCDYSLAELEPRLFSFNNPMGACP
                     KCDGLGNVNFFDPKRVVAFPHLSLASGAIKGWDKRNQFYFQMLTSLANHYAFDIETPF
                     EKLPQTMQEVVLNGSGKEQIPFNYLNERGTIFQRSHSFEGILHNLQRRYHESDSLAVR
                     EELSKYINSTTCPDCAGTRLKREARHVKVGNQNIHQVCEVPLKQALTFFESLELQGAK
                     LAIADKIVKEIQNRLKFLTNVGLEYLSLSRSAETLSGGEAQRIRLASQIGSGLTGVMY
                     VLDEPSIGLHQRDNDRLLDTLKRLRDIGNSVIVVEHDQDAIMSADYVVDIGPGAGEHG
                     GQIVAEGTPAQIQQNAQSLTGLYLSGKKQIEYNKVRTKADPARWLKLNHATGNNLKDV
                     SVEIPVGLLTCITGVSGSGKSTLINDTLYRAVSQHLYGSNAEPSAFGDISGLEFFDKV
                     VDVDQSPIGRTPRSNPATYTGLFTPIRDLFAAVPESRARGYGPGRYSFNVKGGRCEAC
                     QGDGVIRVEMHFLPDVYVPCDVCKGHRYNRETLEIQFKGKNIHEILGMTVEQAHSFFS
                     AQPVIARKLKTLLDVGLGYITLGQSATTLSGGEAQRVKLALELSKRDTGRTLYILDEP
                     TTGLHFADIQLLLDVIHRLRDAGNTIVIIEHNLDVIKTADWIVDMGPEGGDGGGMIIA
                     EGTPEQVAQISQSYTGKYLKAML"
     gene            complement(6504..6980)
                     /locus_tag="PKL42_05970"
     CDS             complement(6504..6980)
                     /locus_tag="PKL42_05970"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HNU66659.1"
                     /translation="MDWLWLLAAILVLVGIAGSILPMLPGIPLVFAGLLMVAWLDGFA
                     KVSVFTIVIIGLIALLALIVEFVASFITVKKAGASQYALWGAAIGGLVGFFVGPFGLI
                     IGTAVGAAIGELLAKKETSQATAVGIAAGLGFVIALVAKVVLLVTMLAIFAYAYYI"
     gene            complement(6984..7724)
                     /gene="mtgA"
                     /locus_tag="PKL42_05975"
     CDS             complement(6984..7724)
                     /gene="mtgA"
                     /locus_tag="PKL42_05975"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HNU66660.1"
                     /translation="MIRWLFNRKPNALPLGFGGVVKRAFVLFILTVFLYQLWIFLHIC
                     WWIKFNPSTSAFMEDRLAVIQESKPDAELKYRWVDYAHISNNLKRAVIASEDAKFNDH
                     EGFDWEGIEHAFEKNLKKGKIVAGGSTISQQLAKNLFLSSKRTPWRKAEEAVITIMLE
                     AMLSKQRILEIYLNVIEWGDGIFGAEAAARHYYKTSAGKLSTAQGAKLAAMIPNPRFY
                     DKHRSTKYLNRRTATIQSRMRFAELPKD"
     gene            complement(7721..8557)
                     /gene="aroE"
                     /locus_tag="PKL42_05980"
     CDS             complement(7721..8557)
                     /gene="aroE"
                     /locus_tag="PKL42_05980"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNU66661.1"
                     /translation="MTDHYAVIGNPISHSKSPNIHTAFGLQTHQDIAYEAKLAPLDGF
                     ATLVHALVKQGYRGANVTVPFKLEAYQLCDTLTERARSAGAVNTLTFAENKIKGDNTD
                     GAGLVNDIEQNLHMRFENKHVLLLGAGGAAQGVLLPILSAHPASLTIANRTIEKAHFM
                     VQSVAATHAVCNINVTTFEHLFPPILKQPFDIVINATSAGLTASHLPISDQVFAQRTL
                     AYDMMYGRETPFMVQARAAGAQVADGLGMLVEQAAEAFYVWRGIRPETATVISHLRKP
                     SN"
     gene            complement(8573..9022)
                     /locus_tag="PKL42_05985"
     CDS             complement(8573..9022)
                     /locus_tag="PKL42_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNU66662.1"
                     /translation="MTTQENILLLAHQRATLNNYPYAGALTPNEAFDVLQNNPSAILV
                     DVRSRAELELVGKVPQASHIEWAFYPGMVANPDFAQTLQAQVDDDLMVIFMCRTGARS
                     HNAAVLAQQLGYSKAYNMLEGFEGEANALKQRTLINGWKHAGLPWTN"
     gene            9352..>9546
                     /locus_tag="PKL42_05990"
     CDS             9352..>9546
                     /locus_tag="PKL42_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HNU66663.1"
                     /translation="MSVANVMKMVEENDIKFVDFRFTDTRGKEQHVTVPVSHFNEDKF
                     TEGHAFDGSSIGGWKGIQASD"
BASE COUNT         2658 a         2251 c         2084 g         2553 t
ORIGIN      
        1 ttctgggcgc gcacgaactt tacaagtcac ttttaaaaca aactcattac gcactgtttc
       61 agcattggca aaagcttgtg gcgtatctgg gtctatcaca atttgcgcca tgccttcgcg
      121 gtcacgcaaa tcaataaaaa taacgccgcc atggtcacgg cgacggtgcg cccagccaca
      181 caatgttacg gtttgtccaa tatgttcgcg gtttaagtgg ccgcagtaat gtgtacgcat
      241 cataataaaa atcttaagtt aaatagttgg taaaaatgtc atggtgtaat tgatagggtt
      301 tggcgaaaac gagtctttat aatttcaacg agaattattt agcccttaag ttttttagcc
      361 gctactaagt cagatggaga aataacgccc atcgaaataa tatatttcaa agcctcatcc
      421 acactcattt caagcgtaat aacgtcttct ttaggtatca tcaaaaagta gccactggtg
      481 gggtttgggg ttgtaggcac ataaacgctg acatgctcac ctcttaaatg aattgcgact
      541 tcaccacccg gctggccagt aataaaggca atggtccaag caccggaatg tgggaattgc
      601 actaaaacag catgccgaaa cgcgttgcca gaatctgaaa acaaagtgtc agatacttgc
      661 tttacacccg cgtaaattga cttcaccacg ggtacgcgat taagtaagtt ctcccataac
      721 gtaatcagtt gcttaccaaa aaaattggta gcgagcacac ccgtcaccag cacaattgcc
      781 accgtcaaga ttgcgcctag gcctggaatg tcacgtttaa aaaggttgtg tgggtgccac
      841 gcatcaggca ataacaacaa gctctggtcc atcacgccaa taaaggtttt taacacccaa
      901 acagtaataa ccagtggcac tagcacgagc aggcctgtaa tgaaatattg ttttattttt
      961 ggcatcatgg tgtttagtgg caggcacacc ctgtgccaca ggctgctggc ggggttgctt
     1021 ctgcaggttt agattctgcc ggttttgttt gagtgctgct ggctgctggt ttttggccgc
     1081 ccttaaagtc ggttgcatac caaccgctac cagttaactg aaagcttggc gctgatactt
     1141 gcttggcaaa agtttcactg ccgcaggcag ggcaaactgt tagatttggt tcactaattt
     1201 tgcgcatcac ctctgctttg tgaccgcaac tactgcattg ataatcgtaa atgggcatag
     1261 tttaaatgac tgctaaattc taaaagcgaa attatacctt acagatagcg ccattgcgat
     1321 gaaacacttt gattttaaaa caatgtttaa attacctgcg gtttatcata tttgccaaca
     1381 cgggcgccaa atattaaaag ccagcattgc tggcttttaa ttgaagcatt tttattttta
     1441 agtaaattct aaaaaggtat gtcatcctca aaatcatcaa aggcatttcc acctgtttgc
     1501 gctggtgctt tttgagcgct tgaagcttgt cttggctgcc cttggcttgg tgcggtttga
     1561 ttataatcat caccgccact ttgatccata ccgccatcat aactcgcatt tgagccgcca
     1621 tctcgcccac ccaacatttg catttggtct gcgataattt ctgttgtgta acgatcttgg
     1681 ccttctttcg tttgccattt gcgcgtttgt agacggcctt caatatacac agggcggccc
     1741 ttttttaaat actcacctgc aatttcagca agtttacgat acatcacaat gttgtgccac
     1801 tctgtgcgct cttgttttgc acctgattta tctttccagt tatcagtcgt ggcaatacta
     1861 aaattacata ctgcttcgcc atttggcata aaacgcacct ctggatcgcg cccgagattg
     1921 cccactagta ttaccttatt cactgatgcc atttttaacc tttcaacaat tgatgcacta
     1981 aagtttctat ttccgcttca ttcacatttt tcgatttatc tatttttaat ataaccgtaa
     2041 gctcttgtgg caacacggcc acttctaaca cgccatgcag gttggttaag ctttgtttaa
     2101 gcgcctcagc ttctgcggcg ggcatattta aagccgattc atccatgcta aacatttttg
     2161 tttttactgc taatggtgct tgcattgaga atgcaaatat tagccataaa atcatcatca
     2221 cgctacaaaa aataaaaaca ctggcatagc caaattgatg cgataaatat ccgccaagtg
     2281 cgccacccac aaaaatgcct atggactggc ttgtattata aacgcccatc gccgtaccct
     2341 ttgcagctgc cggcgcaatt ttactgataa ttgagggtag cgttgcctct aatatgttaa
     2401 acgcaacaaa ataaagtgtg agcgaaaata caatccccca aaaatactga ataaatgccg
     2461 aaaatgctag ttgtgcaatt aacatcaacc caatagcaag tagaaatacc tgcttcattt
     2521 tcgcttgttt ttcagcgtaa ataatcgccg gcaccatgag tacaaatgag ccaattaaaa
     2581 cgggtaaata aatatgccaa tgctgattaa cttcaatctg gctcgacttt gtaatcgcaa
     2641 atggtaccac cacgaacatc gccatttggg cggcatgcag ggcaaaaatg ccataattta
     2701 aacgcaataa ctgcttgttc ttaagcacat cttttaactt agcaggggtg gcactcgcat
     2761 cagaatggtg gtagctgtgc acggggtctg gcaccacaaa ctttactacc gccattgcag
     2821 ccaatgttag caatgcggtc ataaaaaata tgcccggcac gccaatccat tgattgagta
     2881 atggaccgcc cacaagagag attgcaaaag caacaccgat ggtagcgcct atagtagcca
     2941 tggccttggt acgatgctca tcgcgggtaa ggtctgccag caaagctgtc accacagcag
     3001 aaactgcgcc tgcaccttgc agtgcgcggc caataatcac gccttcaata ttcatggcaa
     3061 gggctgccac catactaccc acggcaaaga taccaagccc aagataaatc atcggtttac
     3121 ggccatattt atctgatgcc atgccaaatg gcaattgaaa tagcgcctgc gttaaaccat
     3181 aagcgcctag cgccaagcca atcataaacg gggttggctg ctcaggaaaa gatgcggcat
     3241 aaatcgcaaa aatcggcaaa attgaaaaca tgccgaacat gcgcaagccg taaattgagg
     3301 ccaagctcgt tgtcgcgcga acttcaagcg gggtcatttt ttctgaaatt gccatgtgta
     3361 atgtgaagat taattggtgt gctgaattaa aaatttggac ggttgtattt gcttatttca
     3421 ttactttttc tgacaaattt ttacaagcga tgaaaaattt tgcgcttaat ttataaaagt
     3481 aggtatatta gcaggttgcc aaaaaattaa ctcgagtctt tagtaaaagc atgatggaat
     3541 tcattaaaat tcgcggtgca cgcacgcaca atctcaaaaa tgtctcactg gatattccgc
     3601 gcaatcagct cgtggttatt actgggctat ccggctcggg taaatcaagc ttagcgtttg
     3661 atacgcttta cgcagaaggt cagcgtcgat acgtagaaag tctttcagct tatgcgcgtc
     3721 aatttttggc gcgtatggat aagcccgatg tcgatttgat tgaaggttta tcaccagcca
     3781 tttctattga gcaaaaatcc acctcacaca atccgcgctc gactgtaggc actgtcactg
     3841 aaattcatga ttatttgcgc ctgctctacg cgcgcgctgg tgacccagag tgtcctgacc
     3901 acggcatcaa gcttgaggcg caaacggtca gccaaatggt cgatagcgtg ctcaaactac
     3961 cagaagatac caagttgatg attctcgcac ccgtggtagc caaccgcaaa ggtgagcaag
     4021 tcgatttgtt tgaagaactc aaagcgcaag ggtttgtacg tctacggatt gacggcaaaa
     4081 tttatgaagt cgatgcgctg ccgcaattgg ccaaaaccag caaacacaat gttgatgtgg
     4141 tggttgaccg cttaaaagtg aaggcggaca tgaagcagcg tattgccgaa tcttttgaaa
     4201 ccgcactgcg gttagccgat ggaaaagcaa tcgcagttga gatggattct gacattgaac
     4261 atttattctc cgccaagttt tcatgcccag tatgtgacta ctcacttgca gaactggagc
     4321 cgcgtttatt tagttttaac aacccaatgg gcgcatgtcc aaaatgtgat gggttgggca
     4381 atgtcaactt tttcgacccc aaacgggtgg tggcttttcc gcatttgtcc cttgcgtcgg
     4441 gcgctattaa aggctgggac aagcgcaatc agttttactt tcaaatgctc accagtttgg
     4501 cgaatcatta tgcatttgat attgaaaccc cctttgaaaa attaccccaa accatgcaag
     4561 aagtggtgtt aaatggtagc ggaaaagagc aaatcccttt taactactta aacgaacgcg
     4621 gcactatttt tcagcgtagt catagctttg aaggtatttt gcataacttg cagcgtcgct
     4681 accacgaaag cgattcactc gctgtgcgtg aagagctatc aaaatacatc aacagcacca
     4741 cttgccctga ttgcgctggc acacggctaa aacgcgaagc gcgccatgtt aaagtgggca
     4801 atcaaaacat tcatcaggtg tgcgaagttc cattaaagca agcgctcact ttttttgaat
     4861 cgcttgaatt acaaggcgcc aaactcgcca ttgccgataa aattgtgaaa gagattcaaa
     4921 accgtttgaa gtttttaacc aatgtcggtt tggaatattt atcactttcg cgctcggctg
     4981 aaacactttc gggcggtgaa gcgcagcgca ttcgcttagc cagccaaatt gggagcggcc
     5041 taacgggtgt gatgtatgtg ctggacgagc cttccattgg tctgcatcag cgcgacaacg
     5101 accgcctgct cgacacactc aaacgtctgc gcgacatagg caatagcgtc attgtggttg
     5161 agcatgatca agatgcgatt atgtcagctg attatgtagt agatatcggg ccaggtgcag
     5221 gcgagcatgg cggccaaatt gtggccgaag gtacgccagc gcaaattcaa cagaatgcac
     5281 aatcgctcac aggactttac cttagcggca aaaagcaaat tgaatataac aaagtgcgca
     5341 ccaaagctga cccagcccgt tggttaaagc ttaatcacgc cacgggtaac aacctaaaag
     5401 atgtgtcagt cgagattcct gttggcttac tcacctgcat taccggggtt tcgggcagtg
     5461 gaaaatcaac gcttatcaat gacacgcttt accgtgccgt gtcgcaacat ttatatggca
     5521 gcaatgccga accatctgct tttggcgaca ttagcggctt agaatttttt gacaaggttg
     5581 ttgatgttga ccaaagcccc attggtcgta cgccgcgctc aaaccccgcc acttataccg
     5641 gcttatttac cccgattcgt gatttattcg ccgccgttcc tgaaagccgc gcccgcggct
     5701 acgggccagg ccgctattca tttaacgtca aaggtgggcg ttgtgaagcc tgccagggcg
     5761 atggcgtgat tcgcgtcgaa atgcactttt tgcccgatgt atacgtgcca tgcgatgtgt
     5821 gcaaaggcca ccgttacaac cgagaaacgc tggagattca atttaaaggc aaaaatattc
     5881 acgaaatatt aggcatgacg gtagagcaag cgcacagctt ctttagtgcg cagcctgtga
     5941 tagcccgtaa actcaaaaca ttgctcgatg tgggtttggg ctatattaca cttgggcaaa
     6001 gcgctacaac cctttcaggc ggcgaggcgc agcgtgtgaa actggcactt gaactgagca
     6061 aacgcgacac tggccgaact ttgtatattt tagatgaacc caccaccggc ctgcactttg
     6121 ctgacattca actattgctc gatgtgattc accgactgcg tgacgcaggc aacaccatcg
     6181 tcattattga gcataactta gatgtgatta aaaccgcaga ctggattgtt gacatgggcc
     6241 cagaaggcgg cgatggcggc ggcatgatta tcgccgaagg tacacctgag caagtggcgc
     6301 aaatcagcca aagttacacg ggtaaatatt taaaagcgat gttgtagata gcgatttaaa
     6361 aagatatttt taacgccaaa atccgccgag gaaaaaataa tgctctggga gcctttattt
     6421 ataaggctcc cagagcatcg aaaaaggttg tgctttaaat gtggcttttt tatctgtttt
     6481 taatgcatca atgaatgctt aaattaaatg taatacgcat atgcaaaaat tgccagcatg
     6541 gtgactagca gcacgacttt tgcaacaagc gcaatgacaa aacctaaccc tgcggcaatg
     6601 ccaactgcag tggcttggct ggtttctttt ttcgcaagca gttctccaat agcagcccct
     6661 acggcggtgc caatgatcaa cccgaacggc ccgacaaaaa agccaactaa tccaccaatt
     6721 gccgcgcccc aaagtgcgta ttgactggcg ccagcttttt tgacggtgat aaatgaagcc
     6781 acaaactcaa caataagcgc taataatgcg ataagcccaa tgatgacgat ggtgaatacg
     6841 ctcactttgg caaagccatc taaccatgcg accatcaata agcctgcaaa taccagcgga
     6901 atgcccggca gcatcggcaa aatagacccc gcaatgccaa ccagcacgag tattgcagca
     6961 agtagccata accaatccat tgcttaatcc tttggcaatt cggcgaaacg catgcggctt
     7021 tggatggttg cggtacggcg atttaaatat ttagttgacc gatgtttatc ataaaaacgc
     7081 gggttaggaa tcatagcggc aagcttagca ccctgcgcag tcgataactt gccggcgctg
     7141 gttttataat agtgccgcgc cgcagcttcg gccccaaaaa taccatcgcc ccattcaatc
     7201 acgtttaaat atatttctaa aatgcgctgt ttgctcagca tggcttctag catgatggtt
     7261 atgacagctt cttctgcctt gcgccaaggc gtacgcttgc tcgataaaaa taggttttta
     7321 gcaagttgct ggctaatggt cgaaccacct gcaacaattt tgcctttttt aagattcttt
     7381 tcaaaggcgt gttcaatgcc ttcccaatca aaaccttcat ggtcgttgaa tttagcatct
     7441 tcactggcaa tcacggcgcg ctttaagtta tttgaaatat gcgcgtaatc tacccaccga
     7501 tacttgagtt ccgcatccgg tttactttct tgaataaccg ctaggcggtc ttccataaag
     7561 gcgctggttg acgggttaaa cttaatccac cagcaaatat gtaaaaaaat ccacagttga
     7621 tataaaaaaa ccgtgaggat aaaaagcaca aatgcgcgtt taacaacacc gccaaaaccc
     7681 aaaggaagcg cgtttggttt gcgattgaat agccagcgta tcaattagat ggctttctta
     7741 aatgcgaaat cacggtagca gtttctggcc gaatgccgcg ccaaacataa aaagcctcgg
     7801 ccgcttgctc aaccagcatg cctaagccat cggccacttg cgcaccggcc gcacgcgcct
     7861 gcaccataaa gggtgtttct cggccgtaca tcatgtcata ggccaaagtg cgctgcgcaa
     7921 aaacttggtc agaaattggc aaatgcgatg ctgttaagcc agcagaggtg gcgttgataa
     7981 caatatcaaa aggttgcttt aatatcggcg gaaacaaatg ttcaaaggtg gtaacgttga
     8041 tattgcacac cgcatgcgta gcagcaacac tctgcaccat aaaatgcgct ttttctatcg
     8101 ttctattggc aatggtcagg cttgccggat gagctgataa aatgggtaac aacacacctt
     8161 gcgccgcgcc gcccgcaccc aacagcaata catgtttatt ttcaaaccgc atgtgcaaat
     8221 tctgttcaat gtcattgact aaccctgcac catcagtgtt atcccctttg attttattct
     8281 ctgcaaaagt gagagtgttc actgcaccgg ccgaccttgc gcgctcggtt aaagtatcgc
     8341 aaagttgata agcttccagc ttaaaaggca cggtcacatt ggcaccacga tagccttgtt
     8401 tgactaacgc atgtaccaac gttgcaaacc catccagcgg cgcgagtttt gcctcataag
     8461 caatgtcttg atgtgtttga agcccaaagg ctgtatgaat gttgggtgat ttgctgtgtg
     8521 aaattgggtt gcctattacc gcgtaatggt ctgtcatgct ggcattctgc tttcaattag
     8581 tccacggcag gccagcatgc ttccagccgt taatgagtgt gcgctgcttt aaggcattgg
     8641 cctcaccttc aaaaccttca agcatgttat aggctttgct gtaacctaat tgctgcgcca
     8701 acacggcggc attatggctg cgtgcgccag tacggcacat aaaaattacc atcaagtcat
     8761 catccacttg cgcttgtaat gtttgtgcaa aatcagggtt tgccaccatg ccagggtaaa
     8821 aagcccactc tatatgcgaa gcctgtggca ctttccccac caattctagc tcggcacggc
     8881 tacgcacatc taccaaaatg gcagaagggt tgttttgcaa aacatcaaaa gcctcatttg
     8941 gcgttaaagc gcctgcatag ggataattgt ttagtgtggc tcgctgatgc gccagcaata
     9001 aaatgttttc ttgagttgtc atgtaaaaat ctttcttctt ttgccaatgt cattgaaaca
     9061 tgccaatgat tttaatttta atctctacaa agtagcacta ttcccatata tgaaagtata
     9121 acgcgtaatc cgtcattttc ggctgctgca agcttgcgca tcatattggt gcattttttc
     9181 acaatatgca caatttacgt gcagcctaac gcgctgcagt ttgctacatt cactcgcaaa
     9241 tgacctattt tactattttt tagctggcac acttcctgct atcattacgc attaacaatt
     9301 agtactaatc tttatttttc acccatgcgt cctcaacaat aggagacatt catgtctgta
     9361 gcaaatgtaa tgaaaatggt agaagaaaac gacatcaaat ttgtcgattt ccgttttact
     9421 gatacacgtg gtaaagaaca gcacgtgact gtgcctgttt cacattttaa cgaagataaa
     9481 tttaccgaag gccacgcgtt tgacggctct tcaattggcg gctggaaagg cattcaagct
     9541 tctgac
//
LOCUS       DAOQSI010000073         9503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_6592_length_9503_cov_4.429932, whole genome shotgun sequence.
ACCESSION   DAOQSI010000073 DAOQSI010000000
VERSION     DAOQSI010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 9503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9503
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6592_length_9503_cov_4.429932"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /gene="rpsK"
                     /locus_tag="PKL42_05995"
     CDS             <1..196
                     /gene="rpsK"
                     /locus_tag="PKL42_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNU66664.1"
                     /translation="AAEVAGKSAQENGVKNLEVRIKGPGPGRESAVRALNAAGFKITS
                     ITDVTPVPHNGCRPPKKRRI"
     gene            223..852
                     /gene="rpsD"
                     /locus_tag="PKL42_06000"
     CDS             223..852
                     /gene="rpsD"
                     /locus_tag="PKL42_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNU66665.1"
                     /translation="MARNLDPKCRQCRREGEKLFLKAEKCFTDKCAIEKRNFPPGQHG
                     QRRNQRLSDYGVQLREKQKVRRIYGVLEAQFRSYYAEADRKKGITGENLLQLLECRLD
                     NVTYRMGLGGSRTEARQIVRHNSILVNGKRVNIPSYQVKAGDVISVAESSKSQLRIKG
                     ALEAAEQRGFPAWIEVDVKALKGTFKAKPQRDELPPTINESLVVELYSK"
     gene            969..1955
                     /gene="rpoA"
                     /locus_tag="PKL42_06005"
     CDS             969..1955
                     /gene="rpoA"
                     /locus_tag="PKL42_06005"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNU66666.1"
                     /translation="MQNSPTEYLKPRVVDVEVISPLRARVTLEPMERGFGYTLGNALR
                     RVLLSSIPGYAITEVKIDGVVHEYSTLEGVQEDVVDILLNLKGVALKLNTKSETILVL
                     NKSTEGVVTAGDFETGHDAEIVNPNHVIAHLTKGGKINLEVKVAMGRGYQPVPVRQKA
                     NEEDRVLGFIMVDASFSPINKVSYQVESARVEQRTDLDKLVMDVETNGIIEPEQAIRD
                     AARILMGQLSVFADLEGAPSEVEVKSAPQVDPVLLRPVDDLELTVRSANCLKAENIFY
                     IGDLIQRTENELLKAPNLGRKSLNEIKDVLATRGLTLGMKLENWPPVGLEKA"
     gene            1993..2382
                     /gene="rplQ"
                     /locus_tag="PKL42_06010"
     CDS             1993..2382
                     /gene="rplQ"
                     /locus_tag="PKL42_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNU66667.1"
                     /translation="MRHGNSNRKLNRTSSHRAAMFRNMTASLLRHEVIKTTLPKAKEL
                     RKFAEPLITLGKTPTLANRRLAFSRLRDRDIVGKLFGELGPRYLARNGGYLRVLKCGF
                     RNGDNAPMALVELVDRPDTAAEVVVAE"
     gene            complement(2450..2695)
                     /locus_tag="PKL42_06015"
     CDS             complement(2450..2695)
                     /locus_tag="PKL42_06015"
                     /inference="COORDINATES: protein motif:HMM:NF017574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HNU66668.1"
                     /translation="MHTNLELYTTSHCHLCEEAEALLVQVGEELSFNYNKIEISDNED
                     LLTTYGAKIPVLRNMSTQQELCWPFDKQSIYQLIGEI"
     gene            2830..4221
                     /gene="mgtE"
                     /locus_tag="PKL42_06020"
     CDS             2830..4221
                     /gene="mgtE"
                     /locus_tag="PKL42_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNU66669.1"
                     /translation="MADLHEYKESLSENLMQVIALLEEYGFDEGQSHSHDYSRLQRKL
                     DTLHPADVADILEALPLDQRLQVWDLVKAERDGDILLEVSDAVRQSLIADMDSDELLA
                     AAEQLDTDELADLAPDLPKDVLQELLYSLDAQNRARLQSALSYSEDTVGAIMDFDIVT
                     IREDVTLEVALRYMRRIGSLPDHTDKLFVVDRDDILRGVLPLTKLVVSDLDATVTDVM
                     AEDTVTFHPEDIADEAAKAFERYDLVTVPVVDANNKLVGRITVDAVMDYIREETESDM
                     LSLAGLREEEDFFASVWKSVQNRWAWLAINLVTALVASRVIGVFEGSIEKIVALAALM
                     PIVAGIGGNSGNQTTTMIVRGLALGQVASHNMSSLITKELGVALLNGLIWGGVLALIA
                     YALYGNYQLGLVMMAAMTLNLMLAAMMGVVIPLYMNKFGRDPAVGSSVLITAVTDSGG
                     FLIFLGLATIFLL"
     gene            4256..5536
                     /locus_tag="PKL42_06025"
     CDS             4256..5536
                     /locus_tag="PKL42_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNU66670.1"
                     /translation="MAQSTQQIWDEISVDILTRTGLWQICIIIAACAFAWAVNGALRA
                     YVLKRSKDIHGAREALAATVEGINRVLFPLTALSIVWLAKIFLSKLQHTSLLALASNL
                     LLAMAVIRLVVFVLRYVFSPSGWVKKTENAIAASIWVVLALHLSGLLPELLSALDSIS
                     FSAGKSKVTLLLAIQAVLTVVITVIIALWLSRFVENRLMQNTHLNPNMRVVLSKVLRI
                     VLTLFAVLFAFSAIGLDITLLSVFGGALGVGLGFGLQKIASNFISGFILLLDDSMHIG
                     DVITVEGHYGVVNDLRARYLVLKKLDGTEVVIPNETLIASPVINHSFTDHKARVQMPI
                     QVSYDSPLERAMILVNEAAAKHERVLIEPEPRVLLKGFGESGIDLMLSFWIPDPEEGS
                     AILQSEIYLDIWRAFQANSIVIPFPQREVKIVQK"
     gene            complement(5750..5905)
                     /gene="rpmG"
                     /locus_tag="PKL42_06030"
     CDS             complement(5750..5905)
                     /gene="rpmG"
                     /locus_tag="PKL42_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012696028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNU66671.1"
                     /translation="MREKIKLESSAGTGHFYTTTKNKRTMPGKMEIKKFDPVVRKHVL
                     YKETKLK"
     gene            complement(5931..6167)
                     /gene="rpmB"
                     /locus_tag="PKL42_06035"
     CDS             complement(5931..6167)
                     /gene="rpmB"
                     /locus_tag="PKL42_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNU66672.1"
                     /translation="MARVCQVTGKKPMVGNNVSHAQNKTKRRFLPNLQNRKFWVESEN
                     RWVSMRITNNALRTIDKNGIDAVLAEMRAAGQKI"
     gene            complement(6303..6755)
                     /gene="dut"
                     /locus_tag="PKL42_06040"
     CDS             complement(6303..6755)
                     /gene="dut"
                     /locus_tag="PKL42_06040"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNU66673.1"
                     /translation="MSVLIDLKILDDRIRGQFPTYATPGSAGLDLRACIDSPITLNPG
                     ETTLIPTGMAIHIDNSYYAALILPRSGLGHKHGIVLGNLVGLIDSDYQGQLMVSCWNR
                     GNTAFELNPMERIAQLVIVPVMQAEFHVVDDFESSERGEGGFGSTGKH"
     gene            6893..9184
                     /locus_tag="PKL42_06045"
     CDS             6893..9184
                     /locus_tag="PKL42_06045"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNU66674.1"
                     /translation="MKFNITKINTGLLFAGLLSATSSLMAETMDGAEEASVMIAPKVV
                     VTGTRVEQNSFDLPMSIDATDAAQIQEGQLKVNLSESSSRIPGVVVNNRNNPAQDLAI
                     QIRGFGARSAFGVRGVRLYADGIPMTMPDGQGQTGTFNLDTAGRVEYLRGPFSALYGN
                     SSGGVVQIFTKDGAKEPEINAGMTFGSDNTQRYSLGLSGSGEGFDVVVNANTYRSDGY
                     RDQSDTRRETLHGKFNFKLGDDTKLTVVATALDQPDNEDPQGLTKAQMEADRKQANPG
                     AVKFDTRVSRSHEQVGATLEHKFTQDDTVRLMAYYGQRENEQYQSVAVGAQRNDFQGG
                     GVATIDRTFGGADIRWTHNGKIGENDYSVTAGMNYDRMKDDRKGYENFISNRAFGLLP
                     AGVDCGNVANAVVCGVKGHLRRDEVNTAKNFDQYLQGSVDLSQRFTLSGGLRHSKVRF
                     NNDDKYIANAPAYPNSNPDDSGSVTFSETTPVIGAIFKLTDAVNIYANAGESFETPTF
                     VEMAYKAAGAGLNLDLKPAKSRQYEAGIKALIGSNTLVNASVFKIDTDDEIVVQQQAA
                     GRTVYQNAKSSERKGFELSVDSKFDNGLGAYLAYSYLDAEFSSDFTACRPFRGNQTAC
                     NINAIATLPTAPGPGNSGGELIKSGSNIPGTYKQTLYGEVSWKYAPIGFSTALEARMN
                     SKTDVAFKSEYGHASGYTVAAWRGGFTQDLTQWKFSEFVRIDNLFDKDYVGSVRVADL
                     NSSYYEPAPGRTWLLGLNATYKF"
BASE COUNT         2667 a         1865 c         2231 g         2740 t
ORIGIN      
        1 tgctgctgaa gtggctggta aatctgcaca ggaaaatggt gttaaaaatt tagaagtgcg
       61 catcaaaggt cctggcccag gtcgtgaatc agcagtgcgt gcacttaatg cggctggttt
      121 taaaattact agcattacgg atgtgacccc agtgccacat aacggctgcc gtccacctaa
      181 aaaacgtcgc atttaagcga aaaagattac tggagaacta tcttggctag aaatttagac
      241 cctaaatgcc gtcagtgtcg tcgagaaggc gaaaaattat ttttgaaagc agaaaaatgc
      301 ttcactgaca aatgcgcaat tgaaaaacgt aactttccac ccggtcaaca cggtcaacgt
      361 cgtaaccaac gtctgtctga ttatggcgtt caattgcgtg aaaagcaaaa agtacgtcgt
      421 atatacggcg ttttagaagc acaattccgt agttattatg ctgaggcaga ccgtaaaaag
      481 ggcattaccg gtgagaatct gttacagctt ttagagtgcc gtttagacaa cgtgacatat
      541 agaatgggtt tgggtggatc acgtactgaa gcaagacaaa ttgttcgtca taacagcatt
      601 ctagtaaatg gcaaacgtgt caatattcct tcgtatcagg tcaaagcggg tgacgtaatt
      661 agcgtggctg agtcatctaa gtcacaattg cgaatcaaag gcgcacttga ggcggctgaa
      721 caacgtggct tcccagcatg gattgaagtt gacgttaaag cattaaaagg cactttcaaa
      781 gctaagccgc agcgtgatga gttgccacca acaatcaatg agtcactggt tgttgagctt
      841 tactcaaaat aattaagatt taatataaaa catataaaag tgtgagtgtt atatacattc
      901 acaagaatcg atactctact agctagcatc gctagtagag tttaattctt aaatttaaag
      961 gtataactat gcaaaacagt cctaccgaat acttgaaacc acgtgttgtt gatgtagaag
     1021 tcatatcccc attacgcgca cgtgttacat tagagccgat ggaacgtggc tttggttaca
     1081 cactaggcaa tgcgttgcgt cgagttttat tgtcatctat tcctggctat gccatcacag
     1141 aagttaagat tgatggtgtt gtacatgagt attcgacact agaaggcgtt caagaagatg
     1201 tagttgatat tttattaaat ctcaaaggcg tcgcattgaa actcaatact aaatcagaga
     1261 cgatattagt tttgaataag tctactgaag gtgttgtgac cgctggtgat tttgagacag
     1321 ggcatgatgc tgaaatcgtt aatcccaacc atgtgattgc tcaccttact aaaggcggaa
     1381 aaatcaactt ggaagttaaa gtagcgatgg gtcgcggtta tcaaccagtg ccagtaagac
     1441 aaaaagcgaa cgaagaagat cgcgtgcttg gtttcatcat ggtagatgca tcattcagtc
     1501 caatcaacaa agtgagttat caagttgaaa gtgcacgtgt tgagcagcgt acagacttgg
     1561 ataaattggt gatggacgtt gagacgaacg gtatcattga gcctgaacag gctattcgcg
     1621 atgcggcacg tatcttgatg ggtcaattgt cagtatttgc tgatcttgaa ggcgcaccta
     1681 gtgaggttga agtgaaatct gctccacagg ttgaccctgt attgcttcgt ccagtggatg
     1741 atttagaact aacagtccgt tcagctaact gcctgaaagc tgaaaatata ttctatatcg
     1801 gtgatttgat tcaacgtaca gagaacgagc tattaaaagc acctaatctt ggccgtaaat
     1861 cactcaatga aattaaggat gtacttgcaa cgcgtggcct aacattaggc atgaaactag
     1921 aaaactggcc tccagttggg cttgaaaaag cttaactaac cagcataaac aaagaaactt
     1981 taaggaatta ctatgcgtca cggtaatagc aatcgtaaat taaatcgtac tagcagtcat
     2041 cgcgcggcga tgtttcgcaa catgacagca tcactgctgc gccatgaagt gatcaaaacc
     2101 actttgccta aggcaaagga gttgcgtaaa tttgctgaac ctctcattac attaggtaaa
     2161 acgccaactt tagcgaatcg tcgtttagca tttagtcgct tacgtgatcg tgatattgta
     2221 ggtaaattgt ttggcgagct tggtccacgc tatttggcac gcaatggtgg ctatctacgt
     2281 gtattaaaat gtggcttccg taatggtgac aatgcaccta tggcattggt tgagcttgtg
     2341 gatcgcccag atacagctgc tgaggttgtt gtagcagaat aagcgttatc aaaataaaaa
     2401 agccagcagt agctggcttt ttttatgcgg gtgaaagttt caagtgtttt taaatctcgc
     2461 caatcagctg ataaatgctt tgcttatcaa atggccaaca caactcttgc tgtgtagaca
     2521 tgtttctcag aactggtatt tttgcgccat aggtcgtaag taagtcctcg ttatcagata
     2581 tttcaatttt attgtagttg aaacttagtt cttctccgac ttgaaccagt agtgcttctg
     2641 cctcttcaca tagatggcaa tgtgaggttg tgtataactc taagttagta tgcattttgg
     2701 attggattct attctaaatc tataaaaagt aggctatctc gtcaaaatta aatcataata
     2761 gagggataga gtgtattgca tgattttatt caatgctgtt gttatagtcg ggaatttatt
     2821 tggatcatta tggcagatct gcacgaatat aaagagagtc taagtgaaaa cttaatgcaa
     2881 gtcattgcgt tactggagga gtatggtttt gatgaaggcc agtctcattc tcacgactac
     2941 tctaggcttc aacgcaagct agatacactc catcctgccg acgttgctga catactggaa
     3001 gccttgccgc ttgatcagcg acttcaggta tgggacttgg tcaaagctga gcgtgacggc
     3061 gacattcttt tagaagtatc tgatgcagta cgccaatcgc tgatagcgga tatggattcg
     3121 gatgaactgc tcgctgctgc cgagcagtta gatactgatg aattggccga cctagcccct
     3181 gacttgccta aagatgtttt acaagagctt ttatacagcc tagatgcaca aaatagggcg
     3241 cggttgcagt ctgcactttc ttattcggaa gatacagtgg gtgcgattat ggactttgat
     3301 attgtgacaa ttcgcgagga tgtcacactc gaagttgcct tgcgttatat gcgtcgcatt
     3361 ggtagtttac cagaccatac ggataagctt ttcgtagtag accgcgatga tatcttacgg
     3421 ggtgtattgc cactcactaa gcttgttgtg agcgatttag atgcaacggt taccgatgtc
     3481 atggcagaag ataccgtgac ttttcaccct gaagacattg cagatgaggc ggccaaggct
     3541 tttgaacgct acgatttagt cactgtgcca gtggtggatg ccaataacaa actcgttggc
     3601 cgtatcacag ttgatgcggt gatggattat attcgtgaag aaaccgagag tgatatgctg
     3661 tcattagcag gattgcgtga agaagaagac tttttcgcat ctgtttggaa gtctgtgcaa
     3721 aaccgctggg cctggctcgc aatcaatctc gtcactgctt tagtcgcatc aagagtgatc
     3781 ggcgtattcg aaggttcaat tgaaaaaata gttgcgctgg ctgcactcat gccgattgtt
     3841 gctgggattg gtggaaactc tggcaaccaa acgacgacga tgattgtgcg tggcttggca
     3901 ctagggcaag tggcgtcaca caatatgtca tcactgatta ccaaagaact tggcgtagct
     3961 ttgctgaacg gcctaatttg gggtggcgtg ctggctttaa ttgcttacgc tttatatggc
     4021 aactatcagc taggcttggt gatgatggca gcaatgacat tgaatttaat gctggcggca
     4081 atgatgggtg tagtcattcc gctttacatg aataaatttg gccgcgaccc agcggtaggc
     4141 tcaagtgtgc ttattacagc cgtcactgat agcggcggat ttttgatatt tttagggttg
     4201 gcgacaatct ttttacttta gttgaatttt tattactatt tgaatttaaa ctactatggc
     4261 gcaatccaca caacaaattt gggatgaaat ttcagttgat atcttaaccc gaaccgggct
     4321 ttggcaaatc tgcattatta ttgcggcatg tgcatttgct tgggcagtca atggcgcgct
     4381 acgtgcctat gtgcttaagc ggtccaaaga tattcatggc gcgcgtgagg cgttggccgc
     4441 cacggttgaa ggcattaatc gcgtattgtt tccgctaact gcgctgtcca ttgtctggtt
     4501 agccaaaatc tttctttcca aactgcaaca cacaagctta ttagcgctcg ccagtaacct
     4561 actcttagcc atggcagtga tacggttagt ggtgttcgta ctgcgctatg tattttcacc
     4621 gagtggatgg gttaaaaaga ccgaaaatgc catcgcggct agtatttggg ttgtcttggc
     4681 actccattta agtggcttgt tacctgagtt gctcagtgcg ctggatagca ttagttttag
     4741 tgcaggcaaa agcaaagtca ctttactgct cgctatccaa gcggtgctga cagtggtgat
     4801 tacggtcatt attgcgttat ggttaagccg atttgtcgaa aatagattga tgcagaatac
     4861 gcatctcaac ccgaatatgc gcgtggtgtt gagtaaagta ttacgcattg tgctaacact
     4921 atttgcagtg ctatttgcct tttctgccat tggcttggat atcacattac tttctgtatt
     4981 tggtggcgca ctaggcgtgg gtttgggttt tggtctgcaa aaaattgcca gcaatttcat
     5041 tagcggcttt atattactac tagatgactc aatgcacatt ggcgatgtga ttaccgttga
     5101 gggccattac ggcgtggtca atgacctgcg tgcgcgttat ttggtattaa aaaagctaga
     5161 cggtactgaa gtggtgattc caaatgaaac gctgatagcc agccctgtca tcaaccattc
     5221 atttaccgac cacaaagcgc gtgtgcaaat gccaatacaa gtgagctatg acagcccgct
     5281 ggaacgcgca atgatcctag tgaatgaagc agctgccaag catgaacgcg tgctgataga
     5341 gccagagcca cgcgttttac tgaaaggctt tggtgaaagc ggcattgatt tgatgttatc
     5401 gttttggata ccagatccgg aagaaggctc ggcgatactg caatctgaaa tttatttaga
     5461 tatctggcgc gcattccaag cgaatagcat cgtgattcca ttcccgcaac gcgaagtaaa
     5521 aattgtgcag aagtaactta gccacattgt taaaagccgt attttagcct gatttataac
     5581 acaacctttt tcgatgccct gcgagcctta taaataaagg ctcgcagggc ataaaatttt
     5641 tctcgagaga aataagcgct attacaaagc tgtttaagca catcaattac tgcttgatgc
     5701 caacaaaaaa gcctgcattc cgcaggcttt ttttactact taaataaaac tatttaagtt
     5761 ttgtttcttt gtacagcaca tgtttacgca ctactggatc aaatttcttg atttccattt
     5821 tgcctggcat cgtacgtttg tttttagtcg ttgtgtagaa gtgcccagta ccagcactag
     5881 actctaattt gattttttcg cgcatgctat atgctcctag tttttcttac ttagattttc
     5941 tgaccagcag cacgcatttc agcaagcacg gcatcaatgc catttttgtc gatagtgcgt
     6001 agggcattgt tagtaatgcg catgcttacc cagcgatttt cgctttcaac ccaaaatttg
     6061 cggttttgca aattaggtaa aaaacgacgt tttgttttgt tttgtgcgtg agaaacattg
     6121 ttacccacca tcggcttttt gccagttact tgacatacac gtgccataat tcatccttaa
     6181 cgcttgctta aatgctgtgc ttttttagaa agaccgcaat tatagtacga aagcgattat
     6241 tttctcaagg aaaatatgcg ttaaattgat ttaattacat cattttaagc acattttgcc
     6301 gtttagtgct ttcctgtgct gccaaagccg ccttcgccgc gttcagaaga ttcaaaatcg
     6361 tccacaacat gaaactcagc ctgcatcaca ggtacaatga caagctgcgc gatgcgttcc
     6421 atcgggttga gttcaaatgc ggtattgcca cgattccagc aagacaccat gagttggcct
     6481 tggtaatcag aatcaatcag cccaaccaag ttgccaagca caatgccgtg cttatgccca
     6541 aggcctgagc gtggcaaaat caatgccgca tagtagctgt tatcaatatg gattgccatg
     6601 cccgttggaa tcagtgttgt ctctcctgga ttcaacgtaa tcgggctgtc aatacacgca
     6661 cgtaaatcga gccctgctga gccgggcgta gcatacgttg gaaactggcc acgaatacgg
     6721 tcatctaaaa tttttaaatc gataagcact gacataaaac gtaatctttt tgagaaatga
     6781 ttgaatgcgc tattaaacac taattattca agaaaatcac tatggtttaa tagtgttcaa
     6841 ctaggtatca ttgcgcccaa caatacagtt aaggtttatg gggagtttac aaatgaaatt
     6901 taatattaca aaaatcaata caggactatt atttgctggc ttactgagcg caacttcttc
     6961 gttaatggcg gagaccatgg atggtgctga agaagcaagc gtcatgattg cgccaaaagt
     7021 ggtcgtcact ggcacgcgtg ttgagcaaaa tagctttgat ttgccaatgt cgattgatgc
     7081 aacggatgca gcgcaaattc aagaaggtca gttgaaagtt aatttatcag agtcatctag
     7141 ccgcatacca ggtgtggtgg tgaataaccg taataatcca gcacaagatt tagccattca
     7201 aatccgcggc tttggggcac gttcagcgtt tggtgtacgt ggcgtgcgat tgtatgccga
     7261 tggtattcca atgacgatgc ccgatggaca aggtcaaaca ggcacgttta acctagatac
     7321 ggcaggccgg gtagagtatt tgcgcggccc attttcagcg ctttatggca actcatcggg
     7381 tggcgtggtg caaattttca ctaaagatgg tgcaaaagag cctgaaatca atgcaggcat
     7441 gacctttggc agtgataata cacaacgtta cagcttgggt ttaagcggta gcggcgaggg
     7501 ttttgacgtg gtcgtaaatg caaatactta tcgtagcgat ggttaccgtg accaatctga
     7561 cacacgtcgt gaaaccttac acggtaaatt caattttaaa ttgggtgatg ataccaagct
     7621 gacagttgta gcaacggcgc ttgatcagcc agataacgag gatccgcaag ggctaacaaa
     7681 agcccaaatg gaagcagatc gtaaacaagc gaatccaggt gcggttaagt ttgatacgcg
     7741 tgtttcgcgt agccacgagc aggtaggtgc aactttagag cataaattta cgcaagacga
     7801 cacagtgcgc ttgatggcct attatgggca gcgggagaat gagcaatatc aatcagtagc
     7861 agtaggcgca caacgaaatg actttcaagg tggtggcgta gcgaccattg accgcacttt
     7921 tggtggagca gatattcgct ggacacataa tggaaaaatt ggtgaaaatg actacagcgt
     7981 aacagcaggt atgaattacg accgtatgaa agatgaccgc aaaggctatg agaattttat
     8041 ctcaaataga gcatttggtt tgttgccagc aggagttgat tgcggaaatg tagctaatgc
     8101 agtggtttgt ggtgttaaag gtcatttgcg tcgcgatgaa gttaatacag ccaaaaactt
     8161 tgatcaatat ctgcaaggtt cagtggattt aagccaaaga tttaccttaa gcggtggttt
     8221 gcgccacagc aaagtacgct ttaacaatga cgataaatat attgctaatg cgcccgctta
     8281 cccaaatagc aaccctgacg atagcggctc agttactttt agtgaaacaa cgccggtgat
     8341 tggcgcgata tttaagttga ctgatgcggt taacatttat gccaatgctg gcgagagttt
     8401 tgaaaccccc acttttgtag agatggctta taaagcagca ggcgctggtc ttaacttaga
     8461 cttaaaacct gctaaaagtc gtcagtatga ggcgggtatt aaagctttaa ttggtagcaa
     8521 cactttagta aatgcctcag tttttaaaat tgataccgat gatgaaattg tggtgcagca
     8581 acaagcggct ggccgtacgg tttatcaaaa cgcgaaaagt agtgagcgta aaggctttga
     8641 attatctgtg gatagcaagt ttgataacgg cttaggcgcg tacttggctt atagctattt
     8701 agatgctgaa ttctcatccg attttacagc ctgccgacca tttagaggca atcaaacagc
     8761 ctgcaacata aatgcgatag ctacattacc taccgcacct gggcctggta actcaggtgg
     8821 tgaattaatt aaatctggta gcaatattcc tggcacctat aaacaaacct tgtatggtga
     8881 agtttcttgg aaatatgcac ctataggctt ttcaactgca ttagaagccc gcatgaatag
     8941 caaaacagat gttgcattta agtcagaata tggtcatgca tcgggctata ccgtagcggc
     9001 atggcgtggt ggttttacgc aagatttaac tcaatggaaa tttagtgagt ttgtgcgcat
     9061 tgataacttg tttgataaag actacgttgg ctcagtgcgt gtggccgatt taaatagcag
     9121 ttattacgag cctgcccccg gccgtacttg gttgttaggt ttgaatgcta cttataagtt
     9181 ttaagcaata gacggcaata aaaaagcccc gagttttcgg ggctttttta tggttataat
     9241 ttatggttaa aatagcagtt ttaataccaa acctttttat atcctctaga agccttgtgt
     9301 ttattggctt gcaaaggtga aattttttct cgggagtatt ttaaaagtaa tactagttta
     9361 ttattaagtc tgagaaagta gttttgcagt atgttgcata atctctcttg caacttgaat
     9421 tttaggggcg gtttctaaag tatgcgcccc atttttatca agtaacgtga cttggttgtc
     9481 atcacttccc atcgcatcgg ttg
//
LOCUS       DAOQSI010000074         9426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_6691_length_9426_cov_6.201899, whole genome shotgun sequence.
ACCESSION   DAOQSI010000074 DAOQSI010000000
VERSION     DAOQSI010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 9426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9426
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6691_length_9426_cov_6.201899"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            368..1120
                     /locus_tag="PKL42_06050"
     CDS             368..1120
                     /locus_tag="PKL42_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNU66675.1"
                     /translation="MHNTLRIATFNIHKGLTHFNAAFSLHQQRELLRKVHADIVFLQE
                     VRDAHHGHSKRFANWPTAGQMEFLADAVWQDFSYGKNAVYPAGHHGNALLSKFPIGHT
                     FNQDISAHSTEERGMLHSEIHIPNWNTPLHTICVHLGLFAHWRRQQLMAVAEYIEQHV
                     PHDAPLIIAGDFNDWSTRSGQQFASRLHLHEVFEHHNGKPARSFPSWLPVLRLDRIYV
                     RGFQVNHAAVHAGPHFIKLSDHAVLTATLRKL"
     gene            1182..2363
                     /gene="clsB"
                     /locus_tag="PKL42_06055"
     CDS             1182..2363
                     /gene="clsB"
                     /locus_tag="PKL42_06055"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HNU66676.1"
                     /translation="MTTFVDGNHIQLLRNGTEYFPALVVAIDLANHDIYLQTYIYQAD
                     AIGELIGNALMRAAKRGVAVHVLIDGFGSKDLPPYFVKTLTHAGVHVRVYRPKISPWT
                     LKKSRLRRLHQKVVVIDSTIGFVGGINIIDDYDIPEPAPNNVPPRIDYAVRIEGKLMP
                     AITDNVRKLWHRITWTPLRTAKALHQKNKSKKAKVNTAPATPMSAALVIRDNVLHRRD
                     IEEAYLSAIQNARSEIIIANAYFVPGWRFRRALMAAANRGVRVTLLLQGRMEYFLMFA
                     THAFYHEFLKSGIEIFEYHKSFMHSKVAVIDAQWATVGSSNIDPFSLFLAREANVVIN
                     DTTFAAELRSDICHTIESGASQILPHVWARANLLKRASSWLAFGMVRLFLDLIGRRNE
                     E"
     gene            complement(2550..3968)
                     /locus_tag="PKL42_06060"
     CDS             complement(2550..3968)
                     /locus_tag="PKL42_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HNU66677.1"
                     /translation="MLKNWFAAMSLAFIATFNTVGYAKELPDFTELAEKQGATVVNIS
                     VTQNMRSNTVSPFGPEGDEQLQEFFRRFGIPNMPGMPGQNGEAQPDYKSQSLGSGFII
                     SNDGYILTNAHVVSGADEVIVKLSDNREFKAKIIGTDKRTDVALVKIEASGLQKVTIG
                     DPSKLKVGEWVAAIGSPFGLENTMTAGIVSAKGRALPQENFVPFIQTDVAINPGNSGG
                     PLFNLNGEVVGINSQIYSRSGGSMGLSFAIPIDVAIDVSNQLKTSGKITRGWLGIAIQ
                     EITKELAESFGMKNTNGALVAGVEKNGPADKGGLEAGDVILKFDGKPITTSADLPRAV
                     GATKPGKIVPVEVLRKGSAKTLNIGVGEMPSDPNEAAQSSKSAPQAEANKIGLTLKEL
                     TAAQKKKLNGKNGLLVIESTGAAAQAGIRRGDVILGLNNSETQSVEQFNKQINAVATG
                     KTVAVLVQRGENTLYVPIKVTK"
     gene            complement(4030..4548)
                     /locus_tag="PKL42_06065"
     CDS             complement(4030..4548)
                     /locus_tag="PKL42_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HNU66678.1"
                     /translation="MLEEYAVVLACEGKQATLEIERRTACGLCGQKRGCGNATWGKLL
                     GHQQQGFVAENTVNAKVGDSVIVGIDERAVLNTTLYLYVIPLLSMLLVTVLANFLFTN
                     QFYVMLGAISGLLVGFLWVKGYLIGHDQTGVAYSKKYQTKILRFAESDPDNKKGLENC
                     SSAQTITFEEHT"
     gene            complement(4621..5583)
                     /locus_tag="PKL42_06070"
     CDS             complement(4621..5583)
                     /locus_tag="PKL42_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HNU66679.1"
                     /translation="MRNILVLLFAFIAQHGFAAEDDEAAWQMLQKSAVAAHALSYQGI
                     FVCNSGKQSRSVEIKHMFDGQNEFSRNVVLDGAPREVLSQDGNLVIYNPKNEKIVIEK
                     RRGHNMFPVVLPKNLERIKANYRLKMGGIERVAGRDAQVLLLTPKDQLRYQHHFWVDT
                     EFGLILKSVILNHQNEAIDQIGFSQLNLFNTLNLDWFKPKIDANKDYVMEEENATDVD
                     TSLSPHWMLKDLPAGYRKVEQMVRKVRGKDFPVTHVVFSDGLASVSLFIEPLAKDVKP
                     KTGASSVGNTNFYSRASGFLQVTSVGEVPAATTASIANAVVFVK"
     gene            complement(5623..6162)
                     /locus_tag="PKL42_06075"
     CDS             complement(5623..6162)
                     /locus_tag="PKL42_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HNU66680.1"
                     /translation="MNEQLSALIDDEVSMDEARHLMSALNSSAEVGETWAQYHLIGDV
                     MRGSSTLRSSFKHDLMQKIEQEPTVLSPNAASMKHEATPTFTDEATVVQLPKKIPAYW
                     SVAASLSAVMVVGWMMWQTPVANQNTSMQMASIAPVQPLNTSVQTEVAEIPTEYLVAH
                     QSSAPTASAYFIQTASYSE"
     gene            complement(6243..6863)
                     /gene="rpoE"
                     /locus_tag="PKL42_06080"
     CDS             complement(6243..6863)
                     /gene="rpoE"
                     /locus_tag="PKL42_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HNU66681.1"
                     /translation="MAVQPISSGNREIDHELVLRAQRGDKRAFGLLVDKYQRKLARLL
                     SRMVRDQSEIEDIVQESFIKAYRALPNFRGDSAFYTWLYRIGINTAKNYLVAMGRRPT
                     VSTGVEIEDAENFEDGDELRTTDTPESAMATKQIAQTVNDTVASLPEELRTAITLREI
                     EGLSYEEIATIMACPIGTVRSRIFRARETISLKLKPLLDTPDNKRW"
     gene            7194..8855
                     /gene="nadB"
                     /locus_tag="PKL42_06085"
     CDS             7194..8855
                     /gene="nadB"
                     /locus_tag="PKL42_06085"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNU66682.1"
                     /translation="MQQFDVLIIGSGLAGLTLALRSAADKKVCIVSKRSINDAASDWA
                     QGGISAVLSDDDSIDNHVRDTLTAGGGLCDTAVTRMVAENAKASIEWLIAQGVGFTRE
                     ADNSAYHLTREGGHSHRRIIHAADATGHAVQTTLSEKVKQHPNITVMENHIAVDLITI
                     GKINKRKIVRPGTELNDKNTCLGAYVLDKSTGKVITIAAQNSVLATGGAGKVYLYTTN
                     PDVSTGDGIAMAWRAGCRVANMEFIQFHPTCLYHPHAKSFLISEAVRGEGGLLKLPDG
                     TRFMPEHDRREELAPRDVVARAIDFEMKKRGIDCVYLDITDKPLSFIQAHFPNIYKRC
                     LSFGIDISKVPIPVVPAAHYTCGGVMTDDKGRTDIQNLYAIGETACTGLHGANRLASN
                     SLLECLVFGKAAAEDILSQPVKTSPPLPYWDESRVTDADEEVLITHTWDELRRFMWNY
                     VGIVRTDKRLSRALHRIHMLRDEVHEFYSNFKISNDLIELRNLLQVAELIVESAISRK
                     ESRGLHYSKDHTNLETEPIPTVLEPSNYYSLLDREHGHQRPHVDA"
     gene            8848..9342
                     /locus_tag="PKL42_06090"
     CDS             8848..9342
                     /locus_tag="PKL42_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HNU66683.1"
                     /translation="MRNVWLALIFLSFFTLAGKTEENRYIVTYVYDGDTVKLHPVNTF
                     SQKGDFKLRITDIDAPERNQPFGQKSRRALIQLCQGNHVIATTEVIAKDQYQRALGKL
                     QCNQVDASLHLVNLGLAWYNSKYANDVALYSAEVNARAQKLGLWADKKPIAPWAWRKL
                     NQRQ"
BASE COUNT         2473 a         2296 c         2009 g         2648 t
ORIGIN      
        1 tcaatggcct gcatacgcgc tgggcgctct ggatggttgg ccgtcatttc atgccttaag
       61 catgcggcgt ggctgatgta ggcagttttc attgtggttg gctttagggc agcaggcgtt
      121 ttaaaagcgg tcaatgcgcc aagcgctgcg cccgcgcgta aaaatgttct tctatccaca
      181 gtcagcctat ccttttattt tggtaaaaca gtgcgtgata cgaccaaatg atacgctaaa
      241 aattcatcct ttttttgctt cactaaccca gctttttaaa agcgtgtggc tgttgggtca
      301 gtcagcaata atgcaccact tttgttgtat gataaagtat tcattattgc cagctaacga
      361 gcaacatttg cataatacat tacgcattgc cacctttaat atccataaag gattaaccca
      421 ttttaatgcg gctttttcgt tgcaccagca gcgcgagctg ttgcgcaaag tgcatgccga
      481 cattgtgttt ttgcaagaag tgcgcgacgc gcatcacggc catagcaaac gctttgccaa
      541 ttggccaaca gcggggcaaa tggagttttt ggctgatgcc gtttggcaag atttttcata
      601 cggaaaaaac gcggtttacc ctgccgggca ccatggcaat gcgttgttgt ccaagtttcc
      661 gattgggcac acgtttaacc aagatatttc agcgcacagc actgaagagc gcggtatgtt
      721 acacagtgaa attcatattc caaactggaa cacgccgctt cacaccattt gtgtgcattt
      781 aggtctgttt gcgcattggc gccgtcaaca attgatggct gtagcagagt atattgagca
      841 gcatgtaccg cacgatgcgc cattgattat tgccggtgat tttaacgact ggagcacgcg
      901 ttctggccaa cagtttgcta gtcgcctgca tttgcatgaa gtgtttgagc atcacaatgg
      961 caagcctgcc cgtagttttc catcttggtt gcctgtgttg cggttagacc gtatttatgt
     1021 gcgcggcttt caggtgaacc atgcagcggt gcatgcaggg ccgcacttta taaaattatc
     1081 agaccacgcg gtattgaccg ccacactgag aaagttgtaa tggataaatt taagtgaaat
     1141 tgccaacgct tgattattta ctggacgcct gaccacacac gatgaccact ttcgtcgatg
     1201 gaaatcatat acagctgtta cgcaacggta ccgagtattt tcctgcgtta gtggtggcca
     1261 ttgacctcgc taaccatgat atttatctac aaacttacat ctaccaagca gatgccattg
     1321 gcgaactgat tggcaatgct ctcatgcgtg ccgccaagcg cggcgttgcc gtacatgtac
     1381 tcatagacgg gtttggcagc aaagatttac cgccctattt tgtaaagaca ctcacacacg
     1441 cgggcgtgca tgtgcgcgtt taccgcccca aaatttcgcc atggacactc aaaaaaagcc
     1501 gtttacggcg cttgcatcaa aaagttgtgg tcatcgacag caccatcggt tttgtcggcg
     1561 gcatcaacat tattgatgac tacgacattc cggaaccggc gcccaacaat gtgccgccac
     1621 gtatagatta cgcggtgcgt attgaaggca aactcatgcc agcaatcacc gataatgtgc
     1681 gtaaattatg gcatcgcatt acatggacac cattacgcac cgcaaaagcc ctgcatcaaa
     1741 aaaacaaaag taaaaaagcc aaagtgaaca ccgctcccgc cacgcccatg agcgccgcac
     1801 tggtgattcg agataacgtg ctgcaccgcc gcgatattga agaagcctat ttaagtgcca
     1861 ttcaaaacgc gcgttctgaa atcatcatcg ccaacgccta ttttgtgcct ggctggcggt
     1921 ttcggcgtgc cttgatggct gcggccaatc gcggtgtgcg cgttactttg ctactgcagg
     1981 gccgtatgga atattttttg atgtttgcaa cgcacgcgtt ttatcacgaa tttttaaaaa
     2041 gcggcattga aatttttgaa taccacaaaa gttttatgca cagcaaagtt gcagtgattg
     2101 acgcacagtg ggccacggtt ggttcatcca atattgaccc ttttagttta tttttagcac
     2161 gtgaggccaa cgtggtaatc aatgacacca cctttgctgc cgaattacga agcgacatct
     2221 gccacaccat cgaaagcggc gccagccaaa ttttgccgca tgtttgggcg cgcgccaatc
     2281 tgctgaaacg cgcctcttca tggctcgctt ttggcatggt gcgcttattt ttagacctga
     2341 ttggcagaag aaatgaagaa tgaatttttg cttaattttt aagcaaaata acacccaacc
     2401 ttttttgatg ctctgtgagc cttataaata aaggctcaca gagcatttat tttttctcgt
     2461 gcgattttct attaggctaa caagtaactt tagaactcaa tacacataaa aaaacccgcc
     2521 ataaggcggg ttcggtttaa tgcattgcgt tacttcgtta ctttaatcgg cacatacaag
     2581 gtattctcac cacgctgtac caacaccgcc acggttttac ccgtagcgac cgcattgatt
     2641 tgcttgttaa actgctcaac gctttgcgtt tcagaattat ttaatcctaa tatcacgtca
     2701 ccacggcgaa tacctgcctg cgcagctgcg ccggtacttt caatcaccag caagccgttt
     2761 ttgccgttga gctttttctt ttgtgcggca gtcagctctt tgagcgttaa gcctatttta
     2821 ttcgcctcag cctgtggcgc tgatttactg gattgtgcgg cttcatttgg gtcgcttggc
     2881 atttccccca cgccaatatt gagggtttta gcgctgcctt tacgcaacac ttccactggc
     2941 acaattttgc cgggttttgt tgccccgact gcacgcggca aatcggctga agtggtaatt
     3001 ggcttgccat caaattttaa aatgacatcg cctgcttcta ggccgccttt gtctgccggg
     3061 ccattttttt caacgcctgc caccaacgcg ccattggtgt ttttcatacc aaaagattcc
     3121 gccaattctt tggtgatttc ttgaatggca atacccagcc agccgcgcgt aattttgcct
     3181 gaagttttca attgatttga aacatcaatc gctacgtcaa ttggaatggc aaaagataag
     3241 cccatcgaac caccactacg gctgtagatt tgtgagttaa tacctaccac ttctccattt
     3301 aaattaaaca gcgggccgcc agagttgccc ggattaatgg caacatcggt ttgaataaac
     3361 ggcacaaaat tttcttgtgg caaggcacgg cctttggcac ttacaatccc tgccgtcatg
     3421 gtgttttcta ggccaaaagg tgagccaatg gcagcaaccc attcacctac tttaagtttt
     3481 gaggggtcac caatggtcac tttttgtaag ccagacgctt caattttcac caaagccacg
     3541 tcagtgcgtt tgtcagtgcc aatgattttg gctttaaatt cacggttatc agagagtttg
     3601 acgatgactt catctgcacc gctcaccacg tgcgcattgg ttaaaatata gccatcgtta
     3661 ctgataataa agcctgatcc gagtgactgc gatttgtaat caggctgggc ttcaccgttt
     3721 tgtcctggca tgccaggcat gttgggaatg ccgaatcgtc tgaaaaattc ctgtaactgc
     3781 tcatcgccct cagggccaaa tggcgatacg gtattgctac gcatattttg cgttacacta
     3841 atgttgacga cggtagcgcc ttgtttttct gccagttcag tgaagtctgg caactctttt
     3901 gcgtatccaa ctgtgttaaa agtggcaatg aaagccaaac tcattgctgc aaaccaattt
     3961 tttaacattt actcacctct tttatatttt ttaaaaaccc tgaatttgcg cgacacattc
     4021 actcttcgtt tatgtgtgtt cttcaaatgt tattgtttgg gcgctgctgc aattttcaag
     4081 cccttttttg ttatctgggt cagattcagc aaaacgcaat atttttgttt ggtacttttt
     4141 gctgtacgcc acacccgttt gatcatgccc aatcagatac ccttttaccc ataaaaagcc
     4201 caccagcaaa cctgatattg cccctagcat cacataaaac tgattagtaa acaaaaagtt
     4261 agccagcact gtcaccaata acatagaaag caacggtatt acatacaaat aaagcgtggt
     4321 gttcagcact gcgcgctcat caatgccaac gataacgcta tcacctactt ttgcgttgac
     4381 ggtattttct gccacaaacc cttgttgctg atgcccaagt aattttcccc aagtagcgtt
     4441 tccgcagcca cgtttttgcc cacaaagacc acacgcggtg cgccgctcaa tttctagcgt
     4501 ggcctgttta ccttcacaag caaggacgac tgcatattct tctaacatga tgtttgaata
     4561 gataaatttt agttaattag atcagcacga acgctacttt ttgcgcttta agaggcatta
     4621 ttattttaca aatacaacgg cattggcaat actggccgtt gtggctgctg gtacttcgcc
     4681 cacagaagtg acttgtaaaa atcctgatgc gcgtgagtaa aaatttgtgt tacccacact
     4741 tgatgcacct gttttaggtt taacgtcttt tgctaatggc tctataaata aagataccga
     4801 tgctaagcca tcagaaaaca ccacatgtgt cacaggaaaa tctttaccgc gaactttgcg
     4861 caccatctgc tcaaccttac ggtagcccgc tggcaagtct tttaacatcc agtgcgggct
     4921 taagctggta tcaacatcgg tcgcgttttc ttcctccatc acataatctt tattggcatc
     4981 aattttaggt ttaaaccaat ctaaattcag cgtattaaaa aggttcagct gactaaagcc
     5041 aatttgatct atcgcttcat tttgatgatt gaggatgact gatttcaaaa taaggccaaa
     5101 ttccgtatct acccaaaagt gatgttgata acgcagttgg tctttaggcg ttaacaataa
     5161 tacttgcgca tcgcgccccg caacacgctc aataccaccc atttttaacc ggtaattggc
     5221 tttaattctt tctaaatttt tgggtaatac gactggaaac atattatgcc cacggcgttt
     5281 ttcaatcaca attttttcat ttttagggtt gtaaatcacc aaattaccat cttggctcaa
     5341 cacttctcgt ggcgcgccat ccagcaccac atttctagag aactcatttt gcccgtcaaa
     5401 catatgctta atttcaaccg agcgagattg tttgcctgag ttgcagacaa aaataccttg
     5461 atagcttaaa gcatgcgctg caactgcaga tttttgcagc atttgccatg ccgcttcatc
     5521 atcctctgct gcaaaaccat gctgggctat gaaagcaaat aataaaacta aaatattgcg
     5581 catttgagcc aacgacttaa acatctttcg tgattctttt cattattctg aatagctcgc
     5641 tgtttgaata aaatatgcgc ttgcagtggg cgctgaagat tgatgcgcca ccaaatattc
     5701 cgtcggtatt tctgcaactt cagtttgcac tgacgtattt aacggttgca cgggcgcaat
     5761 gctagccatc tgcattgaag tgttttgatt tgcaaccggc gtttgccaca tcatccagcc
     5821 caccaccatt actgcggata aagaagccgc cactgaccag tatgcaggga tttttttggg
     5881 taattgcacc accgttgctt catctgtgaa agttggcgtt gcttcatgct tcattgatgc
     5941 tgcattgggc gaaagtacag taggttcctg ctcaattttt tgcatcaaat catgcttaaa
     6001 actgctacgc agcgtggaag atccgcgcat gacatcgcca attaaatgat actgtgccca
     6061 agtttcaccc acttcggcac tgctattgag tgccgacatc aagtgcctag cttcatccat
     6121 cgaaacttca tcatcaatga gtgctgataa ctgttcgttc ataccttacc cctaaacttc
     6181 tactacttta attttaaaaa atattttcaa tcaatctgtt attaatttta acgaagccgt
     6241 acttaccatc ttttattatc aggcgtatca agcaaaggtt ttaatttcaa cgagattgtc
     6301 tcgcgcgccc taaaaatacg tgagcgcact gtgccgatgg gacaagccat gatggtagca
     6361 atctcttcat agctcaaccc ttcaatttca cgtaatgtga tggctgtacg caattcttca
     6421 ggcaaacttg ccactgtatc attcaccgtt tgtgcaattt gcttggttgc catggctgat
     6481 tctggtgtat ccgtggtacg taactcatca ccatcttcaa agttttcggc gtcttcaatt
     6541 tcaaccccag tgctcacggt tggcctgcgc cccattgcca ctaaataatt ttttgcggta
     6601 ttgatgccaa tacgataaag ccaagtgtag aacgcgctat cacccctaaa atttggcagg
     6661 gcgcggtacg ccttaataaa ggactcttga acaatgtctt caatttcaga ctggtcacgc
     6721 accatacgcg ataacaaacg cgctaatttg cgctgatact tatcgaccag caagccaaaa
     6781 gcacgcttat cgccacgctg cgcacgcagc actagctcat ggtcgatttc acgatttcct
     6841 gatgatatgg gttgaactgc cactacgcgc agatgtcctt ttgttggtgt ttgctgaaga
     6901 ttaagcgcat taatttgtgc ttcgtttaag tgaatcaccg cgccgctttg gtgtacactt
     6961 tttttattcg accctttagt ctcatttata gcagaagtat aaccttgtgc cgccggctgt
     7021 actaaattta gttgtgttaa ttctaatatt ttaccaaatt gcatcatgaa gtttcctctc
     7081 aacattcaca cgaaccaaaa ttaatgtttt tgcacctttt tcactaacac gcttgccttt
     7141 tataaagaca gcgatacact ctcgatagtt caacaccttt gtcaaagtct cttatgcaac
     7201 aatttgatgt attgattatc ggtagcggtc ttgcaggctt aaccttagca ctacgctccg
     7261 ccgctgataa aaaagtatgt attgtcagca aacgcagtat caatgatgca gccagcgatt
     7321 gggcgcaagg tggcatttcg gcagtattga gcgatgatga ctccattgat aaccatgtgc
     7381 gagatacgct gacggcaggc ggtggcctgt gtgataccgc tgtcaccaga atggtggccg
     7441 aaaatgccaa ggcttcgatt gaatggctga ttgcgcaggg tgttggcttt acccgcgaag
     7501 cagataacag cgcctaccat ttaacccgcg aaggtggtca cagccaccgt cgcattattc
     7561 acgcggcgga cgccaccggc catgcagttc aaaccacgct ttcagaaaaa gtaaagcagc
     7621 accccaatat tacggtaatg gaaaatcata tcgctgtcga tttgataacc attggaaaaa
     7681 tcaacaaacg caaaatagtc aggcccggca ctgaacttaa cgacaaaaac acttgcttgg
     7741 gcgcctatgt gctcgataaa agcaccggca aagtgatcac cattgccgca caaaattccg
     7801 tgttagccac gggtggcgca ggtaaagtct atctatacac caccaatccg gatgtcagca
     7861 cgggcgacgg cattgccatg gcctggcgtg cgggttgccg agtggccaat atggaattta
     7921 tccagtttca cccaacctgc ttgtaccacc cgcatgcgaa atcattttta atctctgaag
     7981 ccgtacgtgg tgaaggcggt ttattaaagc tacccgatgg cactcgcttt atgccagaac
     8041 atgaccggcg tgaagaactt gccccgcgtg atgtagtagc ccgcgccatt gactttgaaa
     8101 tgaaaaaacg cgggattgat tgtgtttatt tagacattac agacaagcca ttaagcttta
     8161 ttcaagcgca ctttcctaat atttataaac gctgcctgag ttttggcatt gatatcagta
     8221 aagtgccgat tcccgtggtg cctgccgcgc actacacttg cggcggcgtg atgaccgacg
     8281 acaaaggtcg taccgacatt caaaatctgt acgccattgg cgaaaccgct tgcactggct
     8341 tgcatggtgc taatcgtctt gccagcaact cgcttttaga atgcttggta tttggcaaag
     8401 cggcagcaga agatatttta agccagcctg ttaaaacgtc accaccactg ccgtattggg
     8461 atgaaagccg cgtgaccgat gccgacgaag aagtgctgat tacgcatacg tgggatgagt
     8521 tgcgccgctt tatgtggaac tacgtaggca ttgtgcgcac cgacaaacgc ttgagccgtg
     8581 cgctgcatcg catccacatg ctgcgcgatg aagtgcatga gttttacagc aattttaaaa
     8641 tcagcaatga tttaattgaa ctgcgtaact tactgcaagt ggctgaattg attgtagaaa
     8701 gtgcaatttc acgcaaagaa agccgtggcc tgcattacag taaagaccac accaatttag
     8761 aaaccgaacc cattccaacc gtgctagaac cttcaaacta ttacagcctg ctggaccgtg
     8821 aacatgggca tcaaagaccg catgtagatg cgtaacgttt ggttagcctt aattttttta
     8881 agctttttta ctttagcggg caagactgaa gaaaaccgct acattgtcac ctacgtttac
     8941 gatggcgaca ccgtaaaact ccaccccgtg aacaccttca gccaaaaagg tgattttaaa
     9001 ctgcgcatca ccgatataga cgcacccgag cgcaatcagc cctttggcca aaaatcgcgc
     9061 cgtgcgctta ttcaactttg ccaaggtaac catgtaattg ccaccactga agtcatcgcc
     9121 aaagatcaat accagcgcgc gcttggcaaa ttgcaatgca atcaggtcga tgcgagtttg
     9181 catttggtaa accttggcct tgcttggtat aacagcaaat acgcaaacga cgttgcgcta
     9241 tacagtgccg aggttaatgc acgtgcgcaa aaattagggc tttgggcaga taaaaaaccg
     9301 attgcccctt gggcttggcg aaagctaaat caacgccaat aataggtaca aaaataggcc
     9361 caaatcacac gcaaaagcgc cttattttaa gcacaacctt tttcgatgcc ctgcgagcct
     9421 tataaa
//
LOCUS       DAOQSI010000075         9291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_6837_length_9291_cov_5.957557, whole genome shotgun sequence.
ACCESSION   DAOQSI010000075 DAOQSI010000000
VERSION     DAOQSI010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 9291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9291
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6837_length_9291_cov_5.957557"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PKL42_06095"
     CDS             <1..305
                     /locus_tag="PKL42_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HNU66684.1"
                     /translation="ALIQQYCAPLLTEGADTIVLGCTHYPFVREAIRDIVGDSVTLID
                     TGAAVAKQLKRQLAESQLLTTKTQPADVMFWTNNDADNAAKIIEALWGKSASVGRI"
     gene            complement(643..963)
                     /locus_tag="PKL42_06100"
     CDS             complement(643..963)
                     /locus_tag="PKL42_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66685.1"
                     /translation="MNAILWDLVANQLAPALVFYLIVWFSTNKKDGKKIKINGAWHIF
                     GVFLSAFIAGVLRLASIFVVGGETALNSQGGSTYGIFFFIILPIIIALGVTSLIRTKG
                     RYVS"
     gene            complement(1003..1191)
                     /locus_tag="PKL42_06105"
     CDS             complement(1003..1191)
                     /locus_tag="PKL42_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HNU66686.1"
                     /translation="MSKIKEIEVTAKDLPLHCPTDAVAQWSSHPRVFLDVTVTGKVAC
                     PYCGTKYKLKAGEHVKHH"
     gene            complement(1248..2180)
                     /locus_tag="PKL42_06110"
     CDS             complement(1248..2180)
                     /locus_tag="PKL42_06110"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HNU66687.1"
                     /translation="MAIPSSMADRDGVIWYDGKMVNWRDATTHVLTHTLHYGMGVFEG
                     VRAYKTDKGTAIFRLQDHTDRLFRSAHILQMKMPFDKATISEAQRAAVRDNNLESAYI
                     RPMAFYGAEAMGIAAKTLSTHVICAAWSWGAYMGEEAITKGIRVKTSSFSRHHVNITM
                     CKAKANGNYMNSILAHQEAAQDGYQEALLLDVDGFVAEGSGENIFIVRKGKLYTPDLT
                     SALEGITRDTIVTLAKELGIEVIEKRITRDEVYGADEAFFTGTAAEVTPIRELDNRAI
                     GTGVRGEITEKLQSLYFDVVKGKSAKHAHWLTLV"
     gene            2294..2884
                     /locus_tag="PKL42_06115"
     CDS             2294..2884
                     /locus_tag="PKL42_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNU66688.1"
                     /translation="MVQKLIRIFGLALLLSFLAACKPASDAGKLVAQDITGADFGQSF
                     HLTDHTGKPRTMDDFKGKAIALFFGYTHCPDVCPTTMADLKHTMKLLGPRADEVQVLF
                     VTLDPKRDTQEVLAQFVPSFDARFIGLRGSEEEIAATAAVFKIYAKKVEAQGQGGYTI
                     DHSAGMYIFDKNGKIRLYVDYGQQPVDIANDIKQIL"
     gene            complement(3048..3422)
                     /locus_tag="PKL42_06120"
     CDS             complement(3048..3422)
                     /locus_tag="PKL42_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HNU66689.1"
                     /translation="MTFVDSVKTCLAKYADFSGRATRSEYWWFYLAFILFYIAGLVLA
                     GILSSISETLGMIGFGIVALGALGFILPSLAAGVRRLHDVDKSGWFLLLGLIPIVGLV
                     LLYFFVIPGTPGDNRFGAPPAN"
     gene            complement(3510..4220)
                     /locus_tag="PKL42_06125"
     CDS             complement(3510..4220)
                     /locus_tag="PKL42_06125"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNU66690.1"
                     /translation="MSNATFTLPNGETISYLLERRQRRTIGLKITADGLVVHAPKRIF
                     DYQLNLVLQQKSHWILSKLKAREANYIEPIQWLHDEHLLVLGNDVQLNITQHSTSKAA
                     TFAQNKLLIAQQDPNNQTLTCRKVIAWYKKQALVDFSRRLEIFAAKLNVPTPPLTLSN
                     AKSRWGSCNSRGEVRLNWRLLQAPPHIINYVICHELAHLKEMNHSAKFWAVVSSIFPE
                     YKLAEKELKILSPQLHRL"
     gene            complement(4217..4945)
                     /locus_tag="PKL42_06130"
     CDS             complement(4217..4945)
                     /locus_tag="PKL42_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNU66691.1"
                     /translation="MADLILRIRAFLFYLGMFIITPLFALLVILMFPLNNITRSRMAS
                     YWAHWSLAWLKLTCNLSFTVRGRENIPDHPSIILCKHQSAWETIALQVIFPPQIWVLK
                     RELLYIPFMGWAWIAMSAIPINRSGGREALKQLVKHGKDRLARGLWVVIFPEGTRVAP
                     GARGKYHIGGAWLASHTESTVVPVAHNAGLFWRKNAFIKQAGTITVSIGKPIETAGLK
                     ADEVNKRVEDWIENEMLQLPQSAE"
     gene            complement(4945..>5400)
                     /gene="gmhB"
                     /locus_tag="PKL42_06135"
     CDS             complement(4945..>5400)
                     /gene="gmhB"
                     /locus_tag="PKL42_06135"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HNU66692.1"
                     /translation="IALLNQSGFKVAIATNQSGISRGLFDMATLNAIHDKMHHALSSY
                     GGRIDAIFYCPHAADDHCHCRKPEAGMIEEIGKRFSIDLVGVPAVGDALRDLQAFANA
                     GCQPILVRTGKGEATLAASLTHADKALPPNTWVCADLAEAVHKMIEVDR"
     assembly_gap    5401..5500
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5501..5660)
                     /locus_tag="PKL42_06140"
     CDS             complement(<5501..5660)
                     /locus_tag="PKL42_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HNU66693.1"
                     /translation="MKLVILDRDGVINQDSTQFIKNPDEWIPIPGSLEAIALLNQSGF
                     KVAIATNQS"
     gene            complement(5679..7775)
                     /gene="glyS"
                     /locus_tag="PKL42_06145"
     CDS             complement(5679..7775)
                     /gene="glyS"
                     /locus_tag="PKL42_06145"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNU66694.1"
                     /translation="MNLLLELFVEELPPKALKKLGEAFSGNLFESLKAQGLTAENTVV
                     TSFATPRRLAAHISSVSAQAADKQIAQKLMPVNVGLDASGNATPALIKRLQGLGLDES
                     AVSQLTKQADGKNEALFLEVTQKGAALKEGLQKALDETLAKLPIPKVMTYQLADGWSN
                     VNFVRPAHGLVALHGSEIVPITALGLTAGNTTQGHRFEAKQPSITLKNADTYAEQLKT
                     EGAVIANYDERRAEIVCQLNAAAAKENLKPIEDEALLDEVNSLVELPNVLLGQFEAEF
                     LAVPQECLILTMKANQKYFPLLDANNKLTNKFLIVSNISPADASAVIGGNERVVRPRL
                     ADAKFFFDQDRKKTLESRIAGLDKVVYHNKLGSQGERNQRVVKIAGEIAKLIGDAKLV
                     EKAELAARLSKADLVTDMVGEFPELQGIMGRYYAQHEGLDDDVAFAIEDHYKPRFAGD
                     ELPRNQIGICVALADKLETLVGMFGIGSTPTGDKDPFALRRHALGVIRMLIEKKIAIP
                     IDNFVNVAFRAFPPKILGDAHTDLHMFIFERVAGLLREQGYSAQEVDAVLSKNTLSLD
                     LVPKQLSAVRAFVELPEADSLIAANKRVGNILKKVEGEVKAEVNAKLLQEPAEIALNA
                     ALCKVKPEADKLFEAGDYTASLQALAALKAPVDDFFDNVMVNADDPALKANRLGLLAI
                     LHQTMNRVADLSKLAS"
     gene            complement(7812..8096)
                     /locus_tag="PKL42_06150"
     CDS             complement(7812..8096)
                     /locus_tag="PKL42_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNU66695.1"
                     /translation="MQPCVYILASERNGTLYIGVTSDLIGRMWQHKNDVVEGFTQKYG
                     VHRLVWYEPHATMESAITREKALKKWNRIWKLRLIEQSNLEWRDLLEQLT"
     gene            complement(8137..9078)
                     /gene="glyQ"
                     /locus_tag="PKL42_06155"
     CDS             complement(8137..9078)
                     /gene="glyQ"
                     /locus_tag="PKL42_06155"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HNU66696.1"
                     /translation="MVLTFQQIILRLQEYWDKQGCALLQPYDMEVGAGTSHTATFLRS
                     LGPEPWKAAYVQPSRRPKDGRYGENPNRLQHYYQYQVVLKPAPANILELYLGSLEALG
                     FDLKKNDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCKPITGEIT
                     YGLERLAMYLQGVDSVYDLQYSENVKYGDVFHQNEVEQSTYNFEHSDADFLFTAFTAH
                     EKQALHLIEAKLALPAYEQVLKAAHTFNLLDARGAISVTERAAYIGKIRNIARAVAAS
                     YLESRARLGFPLAPKDWADEVLEKLSKEKLEKEKKAA"
     gene            complement(9110..>9291)
                     /locus_tag="PKL42_06160"
     CDS             complement(9110..>9291)
                     /locus_tag="PKL42_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66697.1"
                     /translation="REKINALRAIIYKARRASKKVASLIGLFFHFFACFFICKFAIKT
                     RIHPHGLSAKNAFLR"
BASE COUNT         2450 a         2064 c         2086 g         2591 t
ORIGIN      
        1 aagcactgat tcaacaatat tgcgcgccat tactgactga aggggctgac accattgtgc
       61 tgggctgcac acattatccg tttgtgcgcg aagccattcg tgacattgta ggcgatagcg
      121 tcaccttgat tgatactggc gccgccgtag ccaagcaact taagcgccaa ttagcagaaa
      181 gtcagctgtt aaccacaaaa actcagccag cagacgtaat gttttggacg aacaatgacg
      241 ctgataatgc ggcaaaaatt attgaagcct tatggggaaa aagtgcatcg gttggccgaa
      301 tttaattagc aatgtttacc tgtaaattcg cttttttgat gccagaatta gtaccgaaag
      361 aaaaatgatg ctctgcgagc ctttatttac aaggcccaca gagcatctta aaaaggttgt
      421 agggctaaag cagtaaatat tagaacttgt agggcatatt agtgaaactt aatacgtcga
      481 aagtggtgtg tgacatgcga cgcattaaaa ttcgttaatg cgccatacgc aggctggaaa
      541 cgcgccgcaa gtgattgaaa tattttggaa ttgttgtgca gaagtgaagc ttttgtaggt
      601 tgggcagaat gcaatgaagc ccaatattta caattaacaa aatcaactta catatcggcc
      661 ttttgtcctg ataagagaag taacacctaa cgcaatgata atgggtagaa taatgaagaa
      721 aaaaataccg taagtactac ctccttgtga gtttagtgct gtttctcctc ctacgacaaa
      781 aattgaagcg agacgtaata caccggcaat aaaagcagat agaaaaactc caaaaatgtg
      841 ccaagcgcca ttgattttta tttttttacc atctttttta tttgttgaaa accaaactat
      901 tagatagaaa accaatgctg gggctaattg atttgcaact aaatcccata atatggcatt
      961 catcataaac tcctaattta gtacagtcgt taattaaatt gcttagtgat gcttaacgtg
     1021 ctcgcccgct ttaagtttgt atttagtgcc gcagtaaggg catgccactt tgccggtgac
     1081 agttacgtct aaaaacacac gtgggtgtga tgaccattgt gcaactgcat cggtcgggca
     1141 gtgtagcggt aaatctttgg cggtgacttc aatttctttt attttggaca tatcttaaaa
     1201 tctccttaaa tcgtcactcc gactttcgtc ggaatgacga ccttggttta aaccagcgtt
     1261 aaccagtgcg catgtttggc tgatttgcct ttaaccacat caaagtacaa actttgcagt
     1321 ttttcagtga tctcaccgcg cacgcctgtg ccaatggcgc ggttatctag ctcgcgaatt
     1381 ggggtgactt cagccgctgt gccggtaaag aacgcttcat ctgcgccgta cacttcatcg
     1441 cgcgtaatgc gtttttcaat cacttcaatg cctaattctt ttgcgagtgt aacaatggtg
     1501 tcgcgggtaa tgccttcaag cgcgctggtt aaatctggcg tataaagttt gcctttgcgt
     1561 acgataaaaa tattttcgcc tgagccttct gccacaaagc cgtctacatc tagcaataat
     1621 gcttcttggt agccatcttg cgcggcttct tggtgcgcta aaatgctgtt catgtagttg
     1681 ccattggctt tggctttaca catggtgatg ttcacgtggt gacgtgaaaa tgacgatgtt
     1741 ttaacgcgaa tccctttggt aatggcttct tcacccatat aagcacccca gctccaagct
     1801 gcgcaaatta catgcgtgct gagtgtttta gcggcaatgc ccatggcttc tgcaccgtaa
     1861 aacgccattg ggcgaatata ggcagattct aaattgttgt ctcgcaccgc agcgcgttgc
     1921 gcctcactga tggtggcttt atcaaacggc attttcattt gcaaaatatg cgcgctgcgg
     1981 aaaagtcggt cagtgtggtc ttgcaaacgg aaaatcgccg tgcctttgtc ggttttatac
     2041 gcgcgcacgc cttcaaacac gcccatgccg taatgcaagg tgtgggtgag tacgtgggtg
     2101 gtggcgtcgc gccagttcac cattttaccg tcataccaaa ttacgccgtc gcggtctgcc
     2161 attgagcttg gaattgccat gaattgctgc cttataaacg aattaaagta aaagcaatat
     2221 tgtaaactaa agggcggaat tcttgtcgca tgttaaaatc aaaaatgtag ataattgaac
     2281 ataattgagg tgtatggtgc aaaaattaat cagaatattc ggcctagcgt tattgcttag
     2341 ctttttggcg gcctgtaagc ctgcaagtga cgcgggcaag ttagttgcgc aagatattac
     2401 cggggcagat tttggccaat catttcacct gactgaccac acaggtaaac cccgcacgat
     2461 ggatgatttt aaaggcaaag ccattgcctt attttttggc tatacgcatt gtccggacgt
     2521 ttgcccaacc acgatggctg atttgaaaca caccatgaaa ttgcttggcc cacgtgccga
     2581 tgaagtgcag gttttatttg taacgctaga ccccaagcgc gatacgcaag aagtattagc
     2641 gcaatttgta ccttcatttg acgcacgctt tattggcctg cgcggcagtg aagaagaaat
     2701 tgccgcaacc gctgccgtgt ttaaaattta tgcaaaaaaa gttgaagcgc aaggccaagg
     2761 tggttacacc atagaccata gtgcaggcat gtatattttt gataaaaacg gaaaaattag
     2821 actgtacgtg gattacgggc aacaacctgt tgatatcgcc aacgacatca aacaaattct
     2881 ataagcttga tgattaaact gcgtgttatt taagtatctt taaacaaggc tgaattcgcc
     2941 cgagcaaaaa acaatgccct gcacgccttg taaataaagg ctcgcagggc agtataaaag
     3001 gttgcatgca caattgatta aaaattgtgc aatttcttta gatgatttta gttggctggg
     3061 ggcgcaccaa aacggttgtc acctggcgta cctggaatca caaagaagta aagtaacact
     3121 aaaccaacga ttgggataag acccaataac aaaaaccagc cacttttatc tacatcgtgt
     3181 aagcgacgca cccctgcggc gagtgaaggt aagataaaac caagtgcgcc taaagcaaca
     3241 ataccaaaac caatcatgcc taatgtttca gatatgcttg ataaaatacc ggccaagact
     3301 aaacccgcga tataaaacaa aataaaagct aagtaaaacc accaatattc tgaacgtgtt
     3361 gcacggcctg aaaagtcagc gtattttgcc aaacaagttt tcactgaatc tacaaaagtc
     3421 attgtttact cccctaagta atttaaaaat tgaccaaaaa acgcactatt atttttggaa
     3481 caacgacaat ctaacatgaa atttgtaatt tacaaacgat gcagttgcgg cgataaaatt
     3541 ttaagttctt tttcggcaag cttgtattca gggaaaatac tgctaacgac agcccaaaat
     3601 ttagcagagt ggttcatctc ttttaaatgt gccaattcat gacaaatcac gtagttgatg
     3661 atgtgcggcg gcgcttgcag tagccgccag ttgagccgca cttcgccgcg gctgttacag
     3721 cttccccagc gcgacttggc gtttgaaagc gtgagtggcg gggtgggcac attcagtttt
     3781 gcggcaaata tttcaagcct gcggctaaaa tcaaccagcg cctgtttttt ataccaggca
     3841 atcactttgc ggcaggttag cgtttggttg ttagggtctt gctgtgcgat taagagctta
     3901 ttttgcgcaa aggtggcggc tttgctagtg ctgtgttggg tgatgttgag ttgcacatca
     3961 ttgcctaaaa ccagcagatg ttcatcatgt aaccattgaa taggttcaat ataattggct
     4021 tcgcgcgctt tgagtttgct taaaatccaa tgcgattttt gttgtaaaac aaggttgagc
     4081 tgatagtcaa aaatgcgttt tggtgcgtgt accaccaagc catctgctgt aatttttagg
     4141 cctatcgtac ggcgttggcg gcgttcaagc aggtaagaaa tggtttcgcc attggggagt
     4201 gtgaatgttg cattgctcat tcagcgctct gtggtagctg cagcatttcg ttttcaatcc
     4261 agtcttcaac tcgcttattc acttcgtccg ctttgagtcc agccgtttca atcggcttgc
     4321 cgatactcac cgtaatggtg ccagcctgtt tgataaaagc atttttgcgc cagaataaac
     4381 cagcattatg cgccactggc accacggtac tttcagtgtg tgaagcgagc cacgcgccgc
     4441 caatatgata tttaccacgc gcgccaggtg ccacacgtgt cccttcagga aaaattacca
     4501 cccaaagtcc gcgcgcaagc cggtctttac cgtgtttgac taattgtttt agtgcctcgc
     4561 ggccgccaga acggttaatg ggaatggcgg acatggcaat ccaagcccag cccatgaatg
     4621 gaatgtagag caattcacgt tttaataccc aaatttgcgg cggaaaaatc acttgcaagg
     4681 caatggtttc ccaagcggat tggtgtttgc ataaaataat ggaaggatgg tcaggaatat
     4741 tctcgcggcc acgtacggta aaacttaaat tgcaggtgag ctttaaccat gctaatgacc
     4801 aatgcgccca atagctcgcc atgcgtgagc gggtgatatt gttgagcggg aacattaaaa
     4861 taaccaataa cgcaaaaaga ggggtgatga tgaacatgcc taagtaaaat aaaaaggcgc
     4921 gtattcttaa aattaagtct gccattaacg atcgacctca atcattttat gtacggcttc
     4981 ggctaagtcg gcacataccc atgtattggg cggcagggct ttgtctgcat gggttaatga
     5041 ggctgcgagt gtggcttcac ctttgccagt gcgcactaaa atgggttggc aacctgcgtt
     5101 agcaaaggcc tgcaaatcgc gcagtgcatc gccaacagcg ggcacgccaa ctaaatcgat
     5161 tgaaaagcgt ttgccaattt cttctatcat gccggcctct ggtttgcggc agtggcagtg
     5221 atcatcggca gcatgtgggc aataaaatat ggcatcaata cggccgccat agctcgacaa
     5281 agcatggtgc attttgtcat ggatggcgtt gagcgttgcc atgtcaaata aaccgcggct
     5341 gatgcccgat tggttcgtgg caatcgccac tttaaagcca gattgattca gtaaggcaat
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccgattggtt cgtggcaatc
     5521 gccactttaa agccagattg attcagtaag gcaatcgctt ctaaactgcc gggaatcgga
     5581 atccactcat caggattttt gatgaattgc gttgaatcct gattgattac accatcgcgg
     5641 tctaaaatga ctagcttcat agttggacga atttcccctt agcttgctaa tttcgacaaa
     5701 tcagccacgc ggttcatggt ttggtgcaag attgccagca agcctaaacg gttggctttc
     5761 agcgccgggt cgtctgcatt gaccatcacg ttatcaaaga agtcatcgac tggggctttg
     5821 agggcagcta atgcttgcaa tgaagcggtg taatcgccag cttcaaaaag tttatcggct
     5881 tcaggcttta ccttgcataa agctgcattg agtgcaattt cagctggttc ttgaagtagt
     5941 ttggcattca cttcagcctt tacttcaccc tctacttttt tcagaatatt gccaacgcgt
     6001 ttgttggctg cgatgaggct atctgcctct ggtagctcaa caaaggcgcg tacggcactg
     6061 agttgctttg gaacgaggtc gagagaaagc gtgtttttac ttagcacagc atcgacttcc
     6121 tgagcagagt agccttgttc gcgtagcaat cctgcaacac gttcaaatat aaacatatgt
     6181 aggtcggtgt gcgcatctcc aagtattttt ggtgggaatg cgcgaaatgc cacatttaca
     6241 aaattatcga tagggatggc aatttttttc tctattagca tgcgaatcac gcccaacgca
     6301 tgccttctga gagcgaaagg atccttatcg cctgttggag tgctgccaat gccaaacatg
     6361 cctactaacg tttctaactt gtctgccagt gcaacacata ttccaatttg atttcgcggt
     6421 aactcgtcgc ctgcgaagcg tggtttatag tggtcttcaa tggcaaacgc cacatcatca
     6481 tccaagcctt catgttgtgc atagtagcgc cccataatgc cttgcaactc tgggaattct
     6541 cccaccatat cagtgactaa atcagctttt gaaagacgtg cggcaagctc agctttttca
     6601 accaatttag catcaccaat cagtttggca atttcaccgg caattttcac cacacgttgg
     6661 ttacgctcac cttggctgcc taatttattg tgatacacca ctttatctaa gcctgcaatg
     6721 cggctttcta aggttttttt gcgatcttgg tcaaaaaaga atttggcatc agccaagcgt
     6781 gggcgcacca cgcgctcgtt accgccaatc accgcgctgg catcggctgg gctgatgttt
     6841 gaaacaatca aaaacttgtt ggtgagcttg ttgttagcat ccaatagcgg gaagtacttt
     6901 tgattcgctt tcatagtgag aatcaagcac tcttgtggca cagctaaaaa ttctgcttca
     6961 aattggccaa gcaatacatt gggcaattca actaatgaat tcacttcgtc cagcaaggct
     7021 tcatcttcaa tcggcttgag gttttctttg gcagccgcag catttagctg gcacacaatt
     7081 tctgcacgtc gctcgtcata gtttgcaatc acagcgcctt cagttttaag ttgctcagcg
     7141 taagtatcgg catttttaag tgtgatgctt ggctgtttag cttcaaagcg gtggccttgc
     7201 gtggtgttgc cagccgttaa gccaagggca gtaatcggca caatttcact gccatgcaaa
     7261 gccaccaggc catgtgcagg ccgcacaaaa ttcacattgc tccagccatc agccaattga
     7321 taagtcatca ctttgggaat cggcaatttt gctagcgttt catctagtgc cttttgtagg
     7381 ccttctttta atgccgcgcc tttttgtgtg acttctaaaa ataacgcttc atttttgcca
     7441 tcggcttgtt tggtgagttg tgaaacagcg ctttcatcca gccccaagcc ttgtaaacgt
     7501 ttgatgagtg ctggcgtggc gttgccgctt gcatctagcc ccacgtttac aggcatgagt
     7561 ttttgtgcaa tttgtttatc tgctgcttgg gcagaaacag atgaaatatg agcagccaaa
     7621 cggcgcggag tggcgaagct tgtcaccacg gtattttctg ccgttaagcc ttgtgctttt
     7681 aagctttcaa acaggttgcc gctaaaagcc tcgcccaatt tttttaaggc tttaggagga
     7741 agctcttcta caaatagttc taggagtaaa ttcattttaa attcgtcatt cccgcgtagg
     7801 cgggaatcca gtcaagtaag ttgttcaagt aaatctcgcc attccagatt tgattgctca
     7861 atcaatcgca atttccaaat cctgttccat ttttttagtg ccttttcacg tgttatcgct
     7921 gattccatgg tggcatgcgg ttcataccaa accagccgat gtacgccata tttctgtgta
     7981 aacccttcta ctacatcatt tttatgttgc cacattcttc ctatcaagtc agaagtcacg
     8041 ccaatatata aagtcccatt tcgctcactt gctaatatgt aaacacaggg ttgcatattc
     8101 actttgccta gattcccgcc tacgcgggaa tgacggctag gcagctttct tttctttttc
     8161 taatttttct tttgagagtt tctccagcac ttcatccgcc cagtctttag gggcaagcgg
     8221 aaagcctaat cttgcgcggc tttctaaata actggcggct acggcgcgag caatgttacg
     8281 aattttgcca atgtaagccg cgcgttctgt cacgctaatt gcaccgcgcg catccagcaa
     8341 attaaacgta tgcgccgctt taagcacttg ctcgtaagcg ggcagggcga gtttggcttc
     8401 aatcaaatgc aatgcctgtt tttcatgtgc ggtaaaggcg gtgaacagaa agtcagcatc
     8461 agaatgctca aagttgtaag tggattgctc cacttcattt tggtgaaaca cgtcaccgta
     8521 tttaacgttt tctgagtact gcaaatcgta cacgctatcc acgccttgca agtacatcgc
     8581 taaacgctca agtccgtagg taatttcgcc ggtaatgggc ttgcaatcaa tgccgcccac
     8641 ttgttgaaaa taggtaaatt gcgtcacttc catgccgttt agccacactt cccaacctag
     8701 gccccacgcg cccagcgttg ggttttccca atcatcttct acaaaacgga tgtcattctt
     8761 tttcaggtca aaacctagcg cttcaagcga acctaaatac agttctaaaa tattggcagg
     8821 tgcgggtttt agtactactt gatattggta atagtgttgc aaacgatttg ggttttcacc
     8881 atagcggcca tcttttgggc gacggctggg ctgcacgtag gcggctttcc aaggctcagg
     8941 cccgagcgag cgcaaaaatg tggcggtgtg tgaagtgcca gcgccaactt ccatatcgta
     9001 aggttgcagc aaggcgcagc cttgtttgtc ccaatattct tggagtctta aaataatttg
     9061 ctgaaaggtg agtaccatgt tgggagttcg ttcaactaaa agcatgattt taccttagaa
     9121 acgcattttt cgcagaaagt ccatgaggat gtatgcgggt tttaatggca aatttacata
     9181 taaaaaagca ggcaaaaaaa tgaaaaaaca agccaattaa gcttgcaacc ttttttgatg
     9241 ctctgcgagc cttataaata atggctcgca gagcatttat tttttctcga g
//
LOCUS       DAOQSI010000076         9156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_7001_length_9156_cov_5.719371, whole genome shotgun sequence.
ACCESSION   DAOQSI010000076 DAOQSI010000000
VERSION     DAOQSI010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 9156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9156
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7001_length_9156_cov_5.719371"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3642
                     /locus_tag="PKL42_06165"
     CDS             <1..3642
                     /locus_tag="PKL42_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HNU66698.1"
                     /translation="LKKTDVTFSWLSIPLLEPHLANLVIREPELTIRLSPNGAIYIAG
                     ISMEGQSKPDLPNWLLRQSQFQIKNAKVIWQDDMRNAPPLSLNQLNLQLHSPPWASLL
                     KNHRISLTATPSIGTSNPIHIQADVYGNDVSQLKEWRGTLEASLKDADMAAFKPWVNY
                     AAITHPIELQAGNGSAEVSLQFDDAQVQALSSKLALDGVKIKLRTQAEAVQLNQLSGK
                     FNWINTTNSQKLNVENLSLAASNGLKIDNVTGSFTDTIAGNRSLNFKLPQVDLALIQP
                     YLLQLPIPAQPLQKLTQLSPKGKLERLAFSWSGNKTQTNTYQIDTKFDGLSISAHEKI
                     PGFSNLTGEINANQKLGKVTLHSTKALLDFKGILRWPIPADKLDGDITWSLNDQSSKI
                     QIKDLKVSNQHLTGSLNASYLMDGNKGGLLDLKGNFGKGNAKYALFYYPTILGETTLH
                     WLDTSILSGRAEDIHLTVKGRLADFPFVDSKNNLDSKLGLFRVTAKVSDSVLEFGTGW
                     PHIEGLGLDLLFEGKRMELNANTGHLLGNQIIKSKTSIAQLDADSPLLVIDSEVKGNV
                     ENAIQFVNKSPVVTVTQGFTEGLKTGGQGKLNLNLKIPLQNVEASQYKGLYQIVEGRM
                     ESESIPTLTNINGAIEFTESNLTAKNINASAFGSPVTLNLTTGKDKTIYVTTKGKISD
                     TSLKQLVNSQGQNNQSLINIANYFSGSTDWAGNITIRKPAVDISIQSNLVGIGSRLPM
                     PMNKASNEALNLRIDKKQTPNNETISVSLGNRMAAKLIRTGENGQFVLDGGVIQVNAI
                     AESEIKPIDGIQRKGLLVSGNLEYLDGDAWRNVIRDISGTEKQKNMLPMPKFDIKINA
                     LDIFDRRINQLRLTNTADKDNLRLSVQSNEISGDLQWSSQNNGKLMARLSNLTVPEAS
                     PNRSKINDKEASLSDFKKLEQDYPALDITAEQFIYNKKNFGALELVAYPQNDNWNIEK
                     LKLIKSDSIISAEGQWNNWVKNPNTYLNITWDIKNLGKTLNQVGYPDTIKGGEGTLSA
                     QLHWPGSPHQFNAKGLNGDLQLDLKKGQFLKVQPGVGRLLGLLSLQSLPRRLTLDFRD
                     LFSNGFAFDRINGNAKISQGVMRSDNFQMSGPAADVAIKGETNLQNETQHLYVKVLPH
                     VSDSLSLAALAGGPLAGAVAFLAQKILKDPLNKIASSEYEIIGTWDNPQEVNAPKAGP
                     EVNQASPLQ"
     gene            3673..4515
                     /locus_tag="PKL42_06170"
     CDS             3673..4515
                     /locus_tag="PKL42_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNU66699.1"
                     /translation="MPSTNSAPKQEIVKIAAIQMASGPQVAANLSEAKRLIESAVKLG
                     AKLVVLPEYFAIMGLKDTDKVAVREQDGSGPIQDFLSTTAKKNKIWIIGGTVPLVSQF
                     PNKVRNSCLVYDDKGKQVARYDKIHLFGLDLGNEHYHEENTIEAGDAVKVVETPFGKI
                     GLSVCYDLRFPELYRAMGEVNIIVVPAAFTDTTGKAHWETLIRARAIENLSYVIAPAQ
                     GGYHVSGRETHGNTMIVDPWGVILDRLPRGSGVVIATMNPKYQESLRKSLPALKHRTI
                     KSVG"
     gene            4545..6020
                     /gene="tldD"
                     /locus_tag="PKL42_06175"
     CDS             4545..6020
                     /gene="tldD"
                     /locus_tag="PKL42_06175"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HNU66700.1"
                     /translation="MKNPENASQSTLAKGTHFEAATEALLTPANLEVPQLQQVLGEMM
                     SHHIDYADLYFQYSRAESWSLEEGQVKSGNFSIDQGVGVRAVSGEKTAFAYSDDITFD
                     ALQLAAKATKSIASLGNAQTAHKIVTRQAPQLYLPQDPIASLSAEAKVKLLERLEQYA
                     RKLDTRITQVIASITGEYEVVMVARHDGVMAADVRPLVRVGINVIAESNGRREQGSAG
                     GGGRFDYGYFTDEVLQDFAKKAVHQAILNLDARPAPAGSMTVVLGAGWPGILLHEAIG
                     HGLEGDFNRKGSSAFSNMIGQQVAAKGITIVDDGTIADRRGSLSMDDEGNPTNRTVLI
                     EDGILRGYIQDTLNARLMNMPITGNARRESYAHIPMPRMTNTYMLNGTTPPEEIIKSV
                     KRGLFAANFGGGQVDITSGKFVFSAAEAYMIEDGKITYPVKGATLIGNGPEVLKRVSM
                     IGNDMALDSGVGTCGKEGQSVPVGVGQPTLKIDGLTVGGTA"
     gene            6365..>9156
                     /gene="mfd"
                     /locus_tag="PKL42_06180"
     CDS             6365..>9156
                     /gene="mfd"
                     /locus_tag="PKL42_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNU66701.1"
                     /translation="MSQTSPAFSNAIPKLGQVGRITLATSGEDALALANLALQLKANK
                     PSQPLVIISANAFDAQRLIEELPYFAPNLSVHLLPDWETLPYDQFSPHPDLISERLTT
                     LYHIAQNACDVIIVPLSTALIRLSPKAFLSANTFMLKKGQKLDIEALRLQCAEAGYHH
                     VTQVISHGEFSVRGGLVDLFPMGSALPYRIDLFDDEIETIRTFDVDTQRSLYPVPEIR
                     LLPAREFPLDESGIALFRSQFREAFEGDPQRAKIYKDVSKGVASGGIEWYLPLFFEQT
                     ATLLDYLPANSLLCLHGDLDHSAQQFWREAQSRYRTLAHDAERPILPPETLLIKTEDF
                     FAATHAFARISLSISKTSNNLPALDIERRAEHPLHKLADFIRQFSGRILIVAESLGRR
                     ETMHQLFTEHGLAVGICETWADFQASKAHVMLGVSTLHAGVALTDQAQPIAIITEAEL
                     YAATVRQQRRREKEKTRSTEGMLKDLSELRINDPVVHEQHGVGRYKGLVSIDFGEGET
                     ELLLLEYFGEDKLYVPVSQLFLISRYSGGPPESAPLHRLGSGSWEKAKKKALKQIRDT
                     AAELLNLYAQRASRRGHAFTLSLHDYEAFCEGFPFEETPDQLEAIENVIKDMQSGRPM
                     DRLVCGDVGFGKTEVALRAAFVAVMGGRQVAVLVPTTLLAEQHYNNFKDRFAEWPIKI
                     AEISRFRTAKEQKEALAGLEAGNIDIIIGTHRLIQKDVKFKNLGLVVLDEEHRFGVRQ
                     KEQLKALRAEVDVLTLTATPIPRTLSMAMEGLREFSIITTPPQKRLSIKTFHTDYSEG
                     IIREAATREFKRGGQVYFLHNEVDTIHSMREKLERILPDARIAVAHGQLRERELEHVM
                     RDFYHQRYNLLLCTTIIETGIDVPTANTIIMNKADMFGLAQMHQLRGRVGRSHHQAYA
                     YLLTDPDRKITVQAQKR"
BASE COUNT         2713 a         2131 c         2090 g         2222 t
ORIGIN      
        1 cttaaaaaaa cggatgtcac attttcatgg ctaagtattc cgctattgga acctcatcta
       61 gcaaacttgg tgattcgtga acctgaactc acgattagac taagccccaa cggcgcaatt
      121 tatattgccg gcatcagcat ggaaggtcaa tcgaaacctg atttacctaa ctggctcttg
      181 cgtcaatcgc aattccaaat taaaaatgca aaagttatct ggcaagatga catgcgaaac
      241 gcgccaccac ttagtctgaa ccaattaaac ttacaattgc atagcccacc ttgggcaagc
      301 ctacttaaaa accatcgtat aagcctaacg gcaacgccaa gtatcggcac cagcaatccc
      361 attcacattc aagctgatgt gtatgggaat gatgtcagcc agctcaaaga gtggcgtggt
      421 acccttgagg ctagcctcaa agatgcggat atggcagcct ttaagccatg ggttaactat
      481 gctgcaataa cacaccccat tgaactacag gcgggcaatg gttcagctga agtctcttta
      541 cagtttgatg atgcacaagt acaagccctc agtagcaagc ttgcgctgga cggtgtgaaa
      601 attaagctaa gaacccaagc agaagcggtg caacttaatc aactgtcagg taaatttaat
      661 tggattaaca ccacaaactc gcaaaagctc aatgttgaaa acctttcttt ggccgccagc
      721 aatggcctca aaattgacaa cgtaacaggc agttttaccg acacaattgc tggcaaccgc
      781 tcactcaatt ttaaacttcc tcaggttgat ttggcattaa tacagccgta cttattacaa
      841 ttaccgatac ctgcccaacc tttgcaaaaa ttaacgcagc tttcgcctaa aggtaaatta
      901 gagcgccttg cttttagttg gagtggtaac aaaacacaaa caaacacata tcaaattgat
      961 actaaatttg acggattaag catctcagca catgaaaaaa tcccgggatt tagcaattta
     1021 acaggcgaaa tcaacgctaa tcaaaagctt ggaaaagtta ccctgcatag caccaaagcg
     1081 ttgcttgatt ttaaaggcat attacgttgg ccaattccag cggacaagtt agatggtgac
     1141 attacttgga gcctcaatga ccaatcgagc aaaattcaaa ttaaagactt aaaggtcagc
     1201 aatcaacatt taactggcag cctaaacgcc agttacttaa tggacggtaa caaaggtggg
     1261 ctactggact taaaaggtaa ctttggtaaa ggtaatgcca aatatgcact attttattac
     1321 ccgacgattt tgggtgaaac cacgctacat tggctggata cgtctatttt atcaggccgc
     1381 gcagaagata ttcacctgac ggtaaaaggt cgtttagctg attttccatt tgtggatagc
     1441 aaaaacaatc tagatagcaa actggggcta tttagagtga ccgctaaagt gagcgatagc
     1501 gttttggagt ttgggaccgg ctggccgcat attgaaggtt taggcctaga tttactgttt
     1561 gaaggcaaac gcatggagct caatgccaat acggggcatt tgttgggtaa ccaaatcata
     1621 aaaagtaaaa cctccattgc acagttagat gctgattcac ctttgctggt tattgatagc
     1681 gaagtaaaag gaaatgttga aaatgccatt cagtttgtga ataaaagccc cgttgtaacc
     1741 gtcacacaag ggtttactga aggcttaaaa accggcggtc aaggtaagtt gaatctaaat
     1801 ttaaaaatcc ctctgcaaaa cgttgaagca tctcagtaca aaggcttata tcaaattgtt
     1861 gaaggacgta tggaatctga gagcattccc actctcacca atatcaatgg cgcaattgaa
     1921 tttacggaaa gtaatttgac ggctaaaaat atcaatgcaa gtgcatttgg tagccctgtc
     1981 acccttaact taaccactgg taaagacaag actatttatg tcacaacaaa aggcaaaatc
     2041 agtgatacca gccttaaaca actcgtcaat agccaagggc agaataatca gtcactcatc
     2101 aatattgcaa actatttcag tggcagcaca gactgggcag gcaatatcac cattcgaaaa
     2161 cctgccgtgg atattagtat tcaatcaaat ttagtcggca ttggctcacg tctacctatg
     2221 ccaatgaaca aagcgtcgaa tgaagcctta aacttgcgaa tcgataaaaa acaaacccca
     2281 aataatgaaa caatctccgt gtcattaggc aacagaatgg cagccaaatt gatacgcact
     2341 ggagaaaacg gccaatttgt gcttgatggc ggggtcattc aagtcaatgc tatcgctgaa
     2401 agtgaaatca aacccattga cggtattcaa cgtaagggtt tgctagtatc aggtaatctt
     2461 gaataccttg atggagatgc ttggcgtaat gtcatacgtg atatatcagg cactgaaaaa
     2521 caaaaaaata tgttgccaat gcctaaattt gatataaaaa tcaatgcgct ggatattttt
     2581 gacaggcgta tcaatcaact gagactgacc aatacagcag acaaagataa cttgcgcttg
     2641 tcggtgcaaa gtaatgaaat ctccggcgat ttacagtggt caagccaaaa taatggcaaa
     2701 ttaatggcta gactctctaa tttaaccgtg ccagaagcct cgcctaatcg aagcaaaata
     2761 aatgacaaag aagcatcact cagcgatttc aaaaagctag aacaagacta ccctgcgcta
     2821 gacattactg cagagcaatt tatttacaac aagaaaaact ttggcgcact cgaacttgtt
     2881 gcttatccgc aaaatgacaa ctggaatatt gaaaaattaa aactcatcaa gtctgacagc
     2941 attatcagcg ctgaaggtca gtggaataac tgggtaaaaa acccaaatac ctatctcaac
     3001 attacttggg atatcaagaa ccttggcaaa acgctcaatc aagtgggata tccagatacc
     3061 atcaaaggcg gcgaaggcac attgagcgcg caattacatt ggccaggaag tccgcaccag
     3121 tttaatgcaa aagggttaaa cggtgatttg caactagacc ttaaaaaagg ccagttttta
     3181 aaggtgcagc caggggttgg ccgacttttg ggtttattaa gcttgcaaag cctgccgcgc
     3241 agactcaccc tagactttag agatttattc agcaacggct ttgcctttga tcgtattaac
     3301 ggcaatgcaa aaatcagcca aggcgtgatg cgaagtgata actttcaaat gtcaggccct
     3361 gctgccgacg ttgccattaa aggcgaaacc aatctgcaaa atgaaacaca gcatttatat
     3421 gtcaaagtgc tgcctcacgt aagtgacagt ctttcacttg ccgctttagc gggcggccca
     3481 ctggctggtg ctgtcgcatt tttagcgcaa aaaatattaa aagatccctt gaacaaaatc
     3541 gcttcaagtg aatatgaaat tattggcact tgggataatc cgcaggaggt caacgcgccc
     3601 aaggcaggac ctgaggtgaa tcaagcttca ccgctccaat aatgtatgat gtaataaaac
     3661 taaggataca aaatgccaag tacaaactca gcccctaaac aagaaattgt aaaaattgca
     3721 gccatccaaa tggcctctgg cccacaagtt gcggctaact tgagcgaggc caaacgcctt
     3781 attgaatctg cggtaaagct tggcgctaaa ctagtggtat tgcctgagta tttcgcgatt
     3841 atgggcttaa aagacaccga taaagtggcc gtgcgcgagc aagatggcag tggccctatc
     3901 caagatttct taagcactac tgccaaaaaa aataaaatct ggatcattgg tggcacagta
     3961 ccgcttgtca gccaatttcc caataaagtt cgcaatagct gcctagttta tgacgacaaa
     4021 ggtaaacaag tcgcccggta cgataaaatc cacctgtttg gcttagattt aggcaatgaa
     4081 cactaccatg aagaaaacac cattgaagcg ggtgatgctg tcaaagtggt agaaaccccg
     4141 tttggtaaaa tcggcctttc tgtttgctac gacttgcgct ttccggaact atatcgcgca
     4201 atgggtgagg tgaatatcat tgttgtgccc gccgcattta ccgatactac aggtaaggca
     4261 cattgggaga cgttaatccg cgcccgtgcc attgaaaact tgagttatgt gatagcaccc
     4321 gctcagggtg gctaccatgt ttcaggccgt gaaacgcatg gtaacacgat gattgttgac
     4381 ccttggggcg tgattttaga tcggctgccg cgcggctctg gcgttgtgat tgctaccatg
     4441 aacccgaaat accaagaaag cctcagaaaa agcttgcccg cattgaaaca tcgtacgatt
     4501 aaatctgtcg gatgataaaa ctcaccaatc aattgaaaac caaaatgaaa aatccagaaa
     4561 acgccagtca atcaacactt gccaaaggca cgcattttga ggcagcaact gaggctttgc
     4621 ttacacccgc aaatctagaa gtgccgcagt tgcaacaagt gctcggtgag atgatgtcac
     4681 accacattga ttacgcagat ttatattttc agtacagccg tgcagaaagc tggagccttg
     4741 aagaaggcca agtgaaatcg ggtaactttt caattgacca aggcgttgga gtacgcgcag
     4801 tgagcggtga aaaaacagct tttgcttact cagacgacat cacctttgat gcattgcaac
     4861 ttgccgcaaa agcgacaaaa tcaatcgcca gtttaggaaa cgcgcaaacc gcgcacaaaa
     4921 ttgttacccg ccaagcaccg caactctatt taccgcaaga cccgattgct tcactttcag
     4981 cagaggccaa agtgaagcta ttggagcgcc tagaacaata tgcccgtaaa ctggacacac
     5041 gcattacaca agtaatcgcc tcaatcactg gggaatatga agtggtcatg gtagcacgcc
     5101 atgacggcgt catggccgcc gacgtgcgcc cattagtgcg tgtaggcatt aacgtcattg
     5161 ccgaaagtaa tggccgccgt gagcaaggct ctgcgggtgg cggcggacgt tttgactacg
     5221 gctattttac cgatgaggtg ttacaagact ttgccaaaaa agcggtgcat caggccattt
     5281 tgaacctcga tgcgcgccct gctcccgcag gcagcatgac cgtagtgcta ggcgcgggct
     5341 ggccaggcat attattgcac gaggccattg gccacggctt agaaggtgac tttaaccgaa
     5401 aaggcagctc agctttttct aacatgatag gccaacaagt ggccgccaaa ggcatcacca
     5461 ttgtggatga tggcactatt gccgaccgcc gtggttcact cagcatggat gatgaaggca
     5521 accccaccaa tcgcacggtg ctgattgaag acggcatttt acgcggctac attcaggaca
     5581 ccctgaatgc gcgactcatg aacatgccca tcaccggcaa cgcaaggcgc gaaagctacg
     5641 cacatatccc catgccgcgc atgaccaaca cttatatgct gaacggcacc acgccacctg
     5701 aagaaattat taaatcggtc aaaagaggcc tttttgccgc caactttggt ggtgggcaag
     5761 tcgatattac cagcggaaaa tttgtattta gcgcggcaga agcctatatg attgaagatg
     5821 gcaaaatcac ctaccccgtc aaaggcgcca cgctcatcgg caacggccca gaagtgctga
     5881 aacgcgtgag catgataggc aacgatatgg cgctagattc tggcgtaggc acttgcggaa
     5941 aagaaggcca aagcgtgcct gtgggcgtgg ggcaaccgac gttgaaaatt gatgggttga
     6001 ctgtgggggg gacggcataa ttataaaaac ggtcatgtta aattacagtt cgcttagaga
     6061 aaaatcgctt ggcgagcatg ttgtactcga ccctgaaaaa tacgtttaac caacacttca
     6121 attctgttta attccatgca taacctttta agcacctctg caagccttta tttataaggc
     6181 tcacagaggc ataaaaatct ctcggggaat atttatcgga ttttaatcat ttttttcatg
     6241 tcacattgct acgcaaacca agcatttcta cgatgaaaaa caagctataa atttccacca
     6301 aaaccgcgtt tttcaaaccc cagccgttat aatctagcct ttaaaaattc atcaataagt
     6361 atttatgtcg cagacttcac cggccttttc aaacgctatt ccaaagttgg ggcaagtggg
     6421 ccgcatcacg cttgccacaa gtggtgaaga tgcgcttgcg ctggcaaatt tggcattgca
     6481 actcaaagcc aacaagccca gccagccgtt ggtaattatt tccgccaatg cttttgatgc
     6541 gcagcgtttg attgaagaac tcccctattt tgcacccaat ttaagcgtac atttgctgcc
     6601 cgactgggaa acgctacctt acgaccaatt ttcgccgcat cctgatttaa tttctgagcg
     6661 gctcaccacg ctgtatcaca ttgcgcaaaa tgcctgtgac gtgattattg tgccgctttc
     6721 aaccgcgctc atccgcttaa gcccaaaggc ttttttgagt gccaatacct ttatgctcaa
     6781 aaaagggcaa aagctggata ttgaagcctt gcgtttgcaa tgcgccgagg cgggttatca
     6841 ccatgtaaca caagtgattt cgcacggtga gtttagcgta cgcggtggct tagttgattt
     6901 attccccatg ggttccgccc tgccttatcg cattgatttg tttgatgacg aaattgaaac
     6961 catccgcacg tttgatgtcg atacgcaacg cagcctatac cccgtgcctg aaattagact
     7021 actgcctgcg cgcgaatttc cgctagatga gtcaggcatt gcgctctttc gtagccagtt
     7081 tcgcgaagcg tttgagggcg acccgcaacg cgcaaaaatt tacaaagatg tcagcaaagg
     7141 cgtggcttct ggcggcattg agtggtattt gccgttattt tttgagcaaa ccgccaccct
     7201 gctcgattac ctacccgcta acagcctact ttgcctgcac ggtgatttag accacagcgc
     7261 tcaacagttt tggcgcgagg cgcaatcgcg ctaccgcacg ctggcacacg atgctgaacg
     7321 ccctattttg ccgccagaaa ccttgttaat taaaacggaa gacttttttg ctgcaacgca
     7381 tgcgtttgcc agaataagcc tcagcattag caaaacgtct aacaatcttc cggcgctcga
     7441 tattgaacgc cgcgcagaac atccgctgca taaattggca gactttattc gccaatttag
     7501 tggccgaatt ttaattgtgg ctgaaagttt aggccgccgt gaaaccatgc atcagttatt
     7561 taccgagcat ggattggctg ttggtatttg tgaaacatgg gcagattttc aggcaagtaa
     7621 agcgcatgtg atgttaggtg tcagcacatt acatgcgggc gtggcactta ctgaccaagc
     7681 acagccaatt gccattatca cagaagccga actctatgcc gccactgtgc gccagcagcg
     7741 ccgccgcgaa aaagaaaaaa cccgcagcac tgagggcatg ctcaaagatt tatctgagtt
     7801 gcgcattaac gaccccgtgg tgcatgagca gcatggggtt ggccgctaca aaggtttagt
     7861 cagtattgat tttggtgagg gcgaaaccga gctcttgcta ttggaatatt ttggtgaaga
     7921 caagctgtat gtacctgttt cacagctatt tttaatttcg cgctattcag gcggcccgcc
     7981 agaaagcgca ccattgcacc gtctaggcag cggtagttgg gaaaaagcca agaaaaaagc
     8041 gctcaagcaa attcgcgata ccgcagccga attacttaac ttgtatgcac aacgggcatc
     8101 gcggcgcggc cacgcattta ccctcagttt gcatgattac gaagcttttt gtgaaggctt
     8161 tccgtttgaa gaaaccccag accagttaga agccattgaa aacgtcatta aagacatgca
     8221 atctggccgc ccgatggacc gattggtatg cggcgatgtg ggttttggca aaaccgaagt
     8281 tgccctgcgc gcagcttttg tggcggtgat gggtgggcgt caagtggccg tgctggtacc
     8341 caccacgcta ttggctgagc agcattacaa taattttaaa gaccgctttg ccgagtggcc
     8401 gattaaaatt gccgaaatat cgcgctttag aaccgcaaaa gaacaaaaag aggcgttagc
     8461 aggcttagaa gcaggcaata ttgacatcat tattggcaca catcgattga ttcaaaaaga
     8521 tgtgaagttt aagaatttag gcttagtggt gctggatgaa gaacaccgct ttggtgtgcg
     8581 ccaaaaagag cagctcaaag cgctgcgtgc cgaagtagac gtgctgacgc tcacggccac
     8641 accgatacca cgcaccctca gcatggcgat ggaaggcctg cgcgagtttt caattattac
     8701 tacgccaccg caaaagcgct tgagtattaa aacttttcat accgattatt ctgaaggcat
     8761 tattcgcgag gcggctacgc gggagtttaa gcgcggcggg caagtgtatt ttctgcataa
     8821 tgaggtggat accatacatt cgatgcgcga gaaattggag cgtatattgc ccgatgcgcg
     8881 cattgctgtg gcacacggcc aattacgcga gcgtgagcta gaacacgtga tgcgcgactt
     8941 ttatcatcag cgctacaacc tgctactgtg tacaaccatt attgaaaccg gtattgacgt
     9001 accaaccgcc aacaccatta ttatgaataa ggccgatatg tttggcttgg cgcaaatgca
     9061 ccaattgcgt ggtcgcgtgg gccgctcaca tcatcaggcc tatgcgtatt tgctgactga
     9121 ccctgaccga aaaattaccg tgcaagcgca aaaacg
//
LOCUS       DAOQSI010000077         9145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_7014_length_9145_cov_5.268757, whole genome shotgun sequence.
ACCESSION   DAOQSI010000077 DAOQSI010000000
VERSION     DAOQSI010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 9145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9145
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7014_length_9145_cov_5.268757"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PKL42_06185"
     CDS             <1..544
                     /locus_tag="PKL42_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNU66702.1"
                     /translation="TAHEWLNAHGDYLYRFALARLRDVHLAEDAVQETFLAAIKNPNF
                     AAQSSPRTWLTGILKHKIIDTMRKGQREIAVTDLTDDEDADMDEFFNARGHWAEKPIA
                     WNMPSDALEQKQFLGILHSCMDKLPKKLAALFLMRDVHETDNEEICKELQITATNAWV
                     MLYRARMSLRKCLEINWTTG"
     gene            561..812
                     /locus_tag="PKL42_06190"
     CDS             561..812
                     /locus_tag="PKL42_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNU66703.1"
                     /translation="MMLSCKSASRLVSQSLDRPLNWQERLALRFHLVICRHCRRFGKQ
                     LQQLRLAVNAMVQQTERDTNITLKPEAKENIANAINQHY"
     gene            846..1238
                     /locus_tag="PKL42_06195"
     CDS             846..1238
                     /locus_tag="PKL42_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66704.1"
                     /translation="MNTTHKALFASVSAVFALSVAAPASAAENPFALKTLSSGYQVAD
                     THDAKMKDGKCGTGKCGANKKTKNKAKDGACNHDKAKEGSCSHDKAKEGSCSAEKMKE
                     GACSAEKMKEGACSAEKMKEGSCGGEKK"
     gene            1271..2137
                     /locus_tag="PKL42_06200"
     CDS             1271..2137
                     /locus_tag="PKL42_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HNU66705.1"
                     /translation="MSQLHDIQGVGLGLKRELIPQIQALYGQEIIRNIDFLEIAPENW
                     IGAGGKYKDQLDWFLARYPIICHGLCLSLGGLEPLNVAFIKQVKAFLHQHEIPLYTEH
                     LSFCSDGGYLYDLLPIPFTEEAVHYVAQRIRQTQDILGQRIGIENASSYVAAPISEMD
                     ELTFLKAVIEEADCLLHLDINNIYVNSVNFNYDAHQLLRGIPNERIVYAHVAGHYQQA
                     PDLLVDTHGENIIDPVWQLLNAAYQQFGVFPTLLERDLNIPPLTTLISEIDKIAAIQQ
                     QYAQKNHAQTVA"
     gene            2118..2915
                     /locus_tag="PKL42_06205"
     CDS             2118..2915
                     /locus_tag="PKL42_06205"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HNU66706.1"
                     /translation="MPKQLPDTTTPLPAFQAYQKQFTQHIRDPKNQPRPANVPAKRMA
                     IYTEIVYNNIEGTLASCFPVTKKILSARQWQQLMRGFMANYQASTPFFREIPAQFLQH
                     LDSVKLNESKLPAFLPNLMHYEWVELALSVQAAPADLERIDSATDDLLLKPIVITPNL
                     FLLQYDYPVHLISPNYQPKQKTAQPTYLLVFRHLELQIKFMELNPITFQLVALLQNES
                     LSGQHALTKLANAHPQISAEVMMQFGAATLAELKENGVILGVKKALF"
     gene            complement(3124..3315)
                     /locus_tag="PKL42_06210"
     CDS             complement(3124..3315)
                     /locus_tag="PKL42_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HNU66707.1"
                     /translation="MTNETEQEIESPCIGVCAMDETTGYCLGCYRTIDEIKTWWDKTN
                     AEKSALLQTLEARVAQSFD"
     gene            complement(3332..6061)
                     /gene="secA"
                     /locus_tag="PKL42_06215"
     CDS             complement(3332..6061)
                     /gene="secA"
                     /locus_tag="PKL42_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNU66708.1"
                     /translation="MISSLFKKIFGSRNDRLVKQYAQKVQKINAFEPAIQALSDDALR
                     AKTEEFKQRFQNGETLEDLLPEAFAVVREGGVRALGMRHFDVQMIGGMVLNAGKIGEM
                     RTGEGKTLVATLPVYLNALTGKGVHVVTVNDYLAKRDAEWMGKLYNFLGLTVGINLSQ
                     MPNELKRQAYAADITYGTNNEYGFDYLRDNMVHEREARVQRGLSYALIDEVDSILIDE
                     ARTPLIISGQADNSIALYNQINEVAAKLIAQTEEEGEGDFWVDEKGQNVVLSEQGHER
                     AEVILTDSGLLSEGSSLYDAANITLVHHLYASLRARNLYHRDQHYVVRDGEIIIVDEI
                     NGRMMPGRRWSDGLHQAVEAKEGVEIQKENQTLASITFQNYFRMYAKLSGMTGTADTE
                     AYEFNQIYGLETVVIPTHRPMQRKDNMDKVYRTAKEKYAAVILDIKDCQSRGQPVLVG
                     TTSIENSELISTLLNAEKLEHQVLNAKQHEREAHVIAQAGRPGVITIATNMAGRGTDI
                     VLGGNPETDIDAVQHDEKLSEAEKETRIAKLKAEWQQRHDAVLAAGGLHIAGTERHES
                     RRVDNQLRGRSGRQGDPGSSRFYLSLEDQLLRIFASDRVSAIMEKLNMPEGEAIEHVW
                     VTRAIENAQRKVEGRNFDIRKQLLEYDDVANDQRKVIYEQRNELLEAVDVGDTIKAMR
                     EDVLISLIATHIPPNSVEEQWDVPSLERELKAETGLEIPLQKMLEDNPDLHEETLRER
                     VLEAANSAYAAKEQLASAEVMRQFERSVMLQSLDNHWREHLAALDHLRQGIHLRSYAQ
                     KNPKQEYKREAFALFEGLLNTVKAEVTKITMLVQVKTEADVEAVEQPVEVENVQYQHA
                     DYDEALANPQSDEEAALLASGQPVVRDGIKVGRNDPCPCGSGKKYKQCHGQLS"
     gene            complement(6197..7111)
                     /locus_tag="PKL42_06220"
     CDS             complement(6197..7111)
                     /locus_tag="PKL42_06220"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNU66709.1"
                     /translation="MNIIFVSNKMAQAKTLSVFHLVLIALALVAIPVLITLMFIVPQE
                     AAAQKGGKSLIPPQLRFSFNNQQKHLDAYALQLGEMQARMMRLDSQAERLGKLAGEKS
                     QDTPLSDSAPQKAVAKTAQDGRGGPLVLHAPMTEADLQKEIATLLSQIEAGTDNLARI
                     EAKLLQQSVLKDLLPNSSPVTAAYNSSSYGWRSDPFTGEKAFHEGLDFPAPTGTLIYA
                     AAGGIVSTSEDTAGYGKLVKIEHGSGLETRYAHCSKLLVKAGERVEKGQVIAEMGSTG
                     RSTGPHLHYEIRLNGNALDPRQYLKAGA"
     gene            7141..7557
                     /locus_tag="PKL42_06225"
     CDS             7141..7557
                     /locus_tag="PKL42_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HNU66710.1"
                     /translation="MLPFNSLLKQPELLALSVKTKEAQIAQSIWLTVSPENLAQWSHA
                     YSLKNKQLSVFADNNAVAAKIKLLIPSLLIKLEKQGCEVTAIRVKVQVKSTPETPTKP
                     VKKLSRHAATELTALAEKLAGSALGDALAKLAKKAN"
     gene            7813..>9145
                     /locus_tag="PKL42_06230"
     CDS             7813..>9145
                     /locus_tag="PKL42_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HNU66711.1"
                     /translation="MQVELIVALSAAALAVLYGVVMSKWITNLPAGNARMQEIAGAIQ
                     QGAAAYLARQYKTIAIVGVILAVLIAIFLGKQTAIGFVVGAVLSGACGFIGMNVSVKA
                     NVRTAQAATGGIAPAFDVAFKGGAITGMLVVGLGLLGVTAFYWYLGADRGTDLNPLIG
                     LAFGSSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNVGD
                     CAGMAADLFETYVVTIIATMVLGGLMLDNEGISAMIYPLMLGAVSIVASIIGCFFVKA
                     SPGMKNVMPALYRGLAVAGLLSLVAFYFVSHQLFPNGFNHAPNLVASANALFGACAVG
                     LVLTAALVWITEYYTGTDYAPVKHIAKASTTGHATNIIAGIGISMKSTALPVISVCIA
                     ILTAYHLAGLYGVAIAATSMLSMAGIIVALDAYGPITDNAGGIAEMVELPSN"
BASE COUNT         2445 a         2177 c         1959 g         2564 t
ORIGIN      
        1 cacagcacac gaatggctta acgcgcatgg cgattattta taccgctttg cgctggcacg
       61 cttgcgcgat gtccatttgg ctgaagatgc agtgcaagaa acttttttag cggccatcaa
      121 aaatccaaac tttgcagcgc aatcttcccc acgtacatgg ctcacgggta ttctcaaaca
      181 taaaattatt gataccatgc gtaagggtca acgtgaaatt gcagtcaccg atttaactga
      241 tgacgaagac gccgatatgg atgaattttt taatgcgaga ggtcattggg ctgagaaacc
      301 aatcgcttgg aatatgccaa gtgatgcgtt ggaacaaaaa cagtttttgg gtatcttaca
      361 tagttgcatg gataagttac ccaagaaact ggccgcgctt tttttaatgc gcgacgtgca
      421 tgaaaccgat aatgaagaaa tttgtaagga actgcaaatt accgcgacca atgcttgggt
      481 gatgttatac cgcgcgagaa tgagcttaag aaaatgttta gaaatcaatt ggacaacagg
      541 ttaagtagag gaagatgaac atgatgcttt cctgcaaatc tgccagccgt ttggtttcgc
      601 aatcgctcga tcggccgctt aactggcaag aaagattggc tttacgcttt caccttgtga
      661 tatgcaggca ttgtcggcga tttggcaaac aattgcagca gttgcgcctt gcagtcaacg
      721 caatggtgca gcaaactgag cgtgacacta acattactct gaagcctgaa gcgaaagaaa
      781 acatcgctaa tgctatcaat caacattatt aattattgaa tttttaattg aaaaggaaac
      841 atcaaatgaa cacgacccat aaagcactat tcgcctcagt tagcgcggta tttgcacttt
      901 cagttgcagc gcctgccagt gctgcagaaa atccatttgc gcttaaaacc ttgtcttccg
      961 gctatcaagt ggccgatacc cacgatgcca aaatgaaaga cggcaaatgt ggcactggca
     1021 aatgtggcgc aaacaaaaaa accaaaaaca aagcaaaaga tggcgcttgc aaccatgata
     1081 aagctaaaga gggttcatgc agtcacgaca aagccaaaga aggttcatgc agtgctgaaa
     1141 aaatgaaaga aggcgcttgt tcagccgaga agatgaaaga aggcgcgtgc tctgctgaaa
     1201 aaatgaaaga aggttcatgt ggcggtgaga aaaaataacg cttgttattt aaggcgcaac
     1261 tcaccatcgc atgtcacaat tgcatgacat tcaaggcgtg ggtttggggt taaagcgcga
     1321 gctcatcccc caaatccaag cgctttatgg ccaagaaatc atccgcaata ttgattttct
     1381 tgaaattgcc cccgaaaact ggataggcgc aggtggcaaa tacaaagacc agcttgattg
     1441 gtttttagcg cgttacccca ttatttgtca tgggctttgc ctttctttgg gtgggttaga
     1501 accgctcaat gtagcgttta tcaaacaagt caaagcgttt ttacaccagc atgaaatacc
     1561 gctttatacc gaacatttaa gcttttgcag tgatggcggc tatttgtatg acttgctccc
     1621 catccctttt acagaagaag ccgtgcatta cgtggcgcag cgcatacgcc aaacgcagga
     1681 tatcttgggc cagcgcattg gcattgaaaa cgcatcaagc tatgttgcgg cacccatttc
     1741 tgaaatggac gagctgactt ttttaaaagc cgtgattgaa gaagcggact gtcttttgca
     1801 cctagacatc aacaatattt atgtgaatag cgttaatttt aactatgatg cgcatcagct
     1861 tttgcgcggc attccaaatg agcgcattgt ttacgcgcac gtggcggggc actatcaaca
     1921 agcgcccgat ttattggttg atacccacgg tgaaaatatc attgaccccg tctggcaact
     1981 attaaacgcc gcctaccagc aatttggtgt ttttcccacg ttattggaac gtgatttaaa
     2041 tatcccgccg ctcacgacac tgatttctga aatcgataaa atcgcagcga ttcaacagca
     2101 atatgcgcag aaaaatcatg cccaaacagt tgcctgacac aacgacgcca ttgccagcat
     2161 ttcaggctta ccaaaagcaa tttacgcaac atattcgtga tccgaaaaat cagccacggc
     2221 ctgccaacgt gccagccaaa cgcatggcaa tttataccga aattgtctat aacaatattg
     2281 aaggcacgct ggcgagttgc tttcctgtta caaaaaaaat cctcagcgca cgccaatggc
     2341 agcagttgat gcgaggcttt atggctaatt atcaagcaag cacgccattt tttagagaaa
     2401 ttcctgcgca gtttttacag catttagata gcgttaagtt gaatgaaagc aaattacctg
     2461 cttttctgcc caatttaatg cactatgaat gggtagaact cgcactttct gtgcaagcgg
     2521 cgcctgctga tttagagcgc atcgattctg caacagacga cttactactg aaacccattg
     2581 tcatcacacc caacctattt ttgttgcaat acgactaccc cgtacacctt atttcgccga
     2641 actatcaacc taaacaaaaa actgcgcagc caacttattt gctggttttt cgtcatctag
     2701 aactgcaaat taagtttatg gaactcaacc ccatcacttt tcaattggtg gcgttgttac
     2761 aaaatgaatc gctgtcaggc caacatgcgc taacgaaatt ggccaacgcg caccctcaaa
     2821 tatcagcaga agtcatgatg caattcggcg cagcaacact ggcagaactg aaagaaaacg
     2881 gtgtgatttt aggcgtaaaa aaagcgctct tttaacttaa aaaagcgctt ttttaacacc
     2941 aacctttttt gatgctctga aagccttata aataaaggct cgcagggcag aaaaaaatcc
     3001 tcgtagtttt tctacgcatt aaaattggtt tttttggatg cgcctttact aaaaacttgc
     3061 atccaattca acggcaattt ttaaatcaaa acttttaaat caaaactttc gaatcaaagc
     3121 tatctaatca aaactttgcg caacgcgtgc ttcaagcgtt tgtagcaatg cgcttttttc
     3181 tgcgtttgtt ttatcccacc aagttttgat ttcgtcgata gtgcgatagc accctaggca
     3241 atagcctgtg gtttcatcca ttgcacacac gccaatgcag ggcgattcaa tttcttgttc
     3301 agtttcattt gtcatgctaa aaaaaaccca cttaacttaa ttgaccgtga cattgcttat
     3361 attttttacc agaaccgcat ggacaagggt cgttacggcc cactttgatg ccatcacgca
     3421 ctacgggctg accactggcc agtaaggctg cttcttcatc tgactgcgga tttgcaagtg
     3481 cttcatcgta atcagcatgc tgatactgta cattttccac ttcaaccggc tgctcaacgg
     3541 cttctacatc cgcttctgtt ttcacttgca ccagcatggt gattttagtg acttctgctt
     3601 taaccgtatt cagtaagcct tcaaataacg caaaggcttc tcgtttgtac tcttgttttg
     3661 ggtttttttg tgcatatgag cgcaaatgta tgccttggcg caaatggtca agtgccgcca
     3721 agtgctcacg ccaatgatta tctaaactct gcaacatgac tgagcgttca aactggcgca
     3781 ttacctctgc actggctaac tgctcttttg ccgcataagc gctgttagcg gcctccagca
     3841 cgcgttcgcg cagggtttct tcatgcaaat cagggttgtc ttccaacatt ttttgcagcg
     3901 gaatttctaa gcctgtttcg gccttcagtt cacgctctaa acttggcacg tcccattgct
     3961 cttccacgct attgggcgga atgtgtgtgg caatcaggct gatgagcaca tcttcacgca
     4021 tggctttaat ggtatcgcca acatccactg cttctaacaa ttcgttgcgt tgctcataaa
     4081 tcactttacg ttgatcgttt gcaacatcat catactcaag taattgttta cggatatcaa
     4141 agttgcggcc ttcgaccttg cgttgtgcgt tttcaatcgc acgtgtgacc cacacatgct
     4201 caatcgcctc gccttcaggc atgttcagtt tttccataat cgcagaaaca cggtcagagg
     4261 caaaaatgcg caacaattgg tcttcaagcg ataagtaaaa acgactggag cctgggtcac
     4321 cctgacggcc agaacgacca cggagttgat tgtctactcg gcgagattca tgacgctcag
     4381 taccggcaat gtgcaggcca cccgctgcca gcacggcatc atggcgctgt tgccactcgg
     4441 ctttaagttt tgcaatgcgt gtttcttttt cagcttcact taatttttca tcgtgctgca
     4501 ccgcgtcaat atcggtttct gggttaccgc ccaacacaat atcggtacca cggcccgcca
     4561 tgttagttgc aatggtaatc acgccagggc ggccggcttg tgcaatcaca tgcgcttcac
     4621 gctcgtgctg tttagcattt aaaacttgat gctcaagttt ttctgcattc aataatgttg
     4681 agatcagttc tgagttttca atcgaagtcg ttcccaccag cactggctga ccgcggcttt
     4741 gacaatcttt aatgtccaaa ataaccgccg catacttctc ttttgcggtg cggtagactt
     4801 tatccatatt gtctttacgc tgcattgggc ggtgtgttgg aatcacgacc gtttctaagc
     4861 cataaatctg attaaattca taagcttctg tatctgcagt acctgtcatc cctgacagtt
     4921 ttgcatacat acggaaatag ttttgaaaag taattgaagc gagcgtttga ttttcttttt
     4981 gaatctcaac gccctctttg gcttcaactg cttggtgcaa accatctgac cagcgacggc
     5041 ccggcatcat acggccgtta atttcatcca caatgataat ttcaccatcg cgcaccacat
     5101 aatgctggtc acgatgatat aaattgcgcg cgcgtagtga ggcatacaag tgatgcacca
     5161 acgtgatatt ggccgcgtca tacaggctag atccttcaga caacaagcct gaatcagtaa
     5221 gaatcacttc tgcacgctca tggccttgtt cagacaacac cacattttgg cctttttcat
     5281 caacccaaaa gtcaccttca ccctcttctt cggtctgtgc gatgagcttt gcagccacct
     5341 cgttaatttg gttgtaaagc gcaatgctgt tatcggcctg ccctgaaata atcagcggcg
     5401 tacgtgcctc atcaattaaa atcgaatcga cctcatcaat cagggcgtaa ctcaagccgc
     5461 gctgcacacg tgcttcacgc tcgtgcacca tgttatcgcg caggtaatca aagccgtatt
     5521 cattattggt gccataggta atatcagccg catatgcctg acgcttcaat tcattgggca
     5581 tttgcgacaa gttaatgcca acagttagcc ctaaaaaatt atagagtttg cccatccact
     5641 cagcatcgcg tttggccaga taatcattca ccgtaaccac atgcacaccc ttgcccgtta
     5701 aggcattaag gtacaccggc aaggtcgcca ccaaggtttt accttcacct gttcgcatct
     5761 caccgatttt tccggcattt agcaccatgc cgccaatcat ttgcacatca aaatggcgca
     5821 tgccgagggc tcgcacgcca ccttcacgca ccacggcaaa ggcctctggc agcaggtctt
     5881 caagcgtttc accattttga aagcgctgtt taaactcttc cgttttggct cttaacgcat
     5941 catcagaaag cgcctgaatc gccggctcaa atgcatttat tttttgtact ttttgtgcgt
     6001 actgtttaac caaacggtca ttacggctac cgaatatttt tttgaacaac gaagaaatca
     6061 tgaagtaagt ggctttccgc tttaaggata taaggcttta atgatgtgta actaaattta
     6121 tgtgaaggtg atgttatgct tgaaaagtgc taaataaaat ttgtcttata ggtacatgag
     6181 ggcattcaag gtaaattcaa gcacccgctt taagatattg tcttgggtcg agcgcattgc
     6241 catttagcct aatttcataa tgcaaatgcg gccctgtaga gcgtcccgtg ctacccatct
     6301 cggcaatgac ttgacctttt tctacgcgtt cacccgcttt caccaacaac tttgaacaat
     6361 gcgcataacg cgtttctagc cctgagccat gctcaatttt gactagttta ccatatcccg
     6421 ctgtatcttc agaggttgac acaatgccac ccgctgctgc atagatcaac gtacccgtcg
     6481 gcgcaggaaa atccagcccc tcatgaaatg ccttttcacc ggtaaaaggg tccgagcgcc
     6541 agccgtaact tgaagagtta taagccgcag tgactgggct gctattaggt aacaaatctt
     6601 ttaacacgct ttgctgcagt aatttggctt ctattctcgc taaattatct gtgcccgctt
     6661 ctatttggct taatagtgtt gcaatttcct tctgcaaatc agcttcggtc attggggcat
     6721 gcaataccag tggaccacca cgaccgtcct gcgctgtctt ggcaacggct ttttgtggag
     6781 cgctgtcact cagcggcgta tcttgtgatt tttcaccggc caactttccc aaacgctctg
     6841 cttgtgaatc cagacgcatc atgcgcgctt gcatttcacc cagttgcaaa gcgtaggcat
     6901 ctaaatgttt ttgttgatta ttaaacgaga atcttaattg cggcggaatc aatgacttcc
     6961 cccccttttg tgctgctgct tcctgtggca caatgaacat caaggtgatt aacactggaa
     7021 tagccaccaa agcaagtgca attagcacaa gatgaaacac agaaagtgtt ttcgcttgcg
     7081 ccatcttatt agagacaaaa atgatattca taaagttatt taatactttt agtggcccgc
     7141 atgcttccgt ttaattcgtt actcaaacag cctgaacttc tagccctcag tgttaagacg
     7201 aaagaagcgc aaattgccca atctatttgg ctgactgtta gccctgaaaa cttagcgcaa
     7261 tggagccatg cctacagttt aaaaaataaa caattgtcag tatttgcaga caacaatgca
     7321 gttgccgcaa aaattaaact tttaatccct agcttattga ttaagctaga aaagcaaggg
     7381 tgtgaagtta ctgcaattcg ggtaaaagtg caagtaaaat ctaccccaga aacccccact
     7441 aaaccagtca aaaagctaag ccgccacgcc gccaccgagt taacggcact agccgaaaaa
     7501 ttagcaggct cagctctagg tgacgcactt gctaagcttg caaaaaaagc aaactaaaca
     7561 attattctct tttgttgcgt atgcaagaaa catccctccg cacccctaaa aatctaacgg
     7621 ttgttcacct tgcttaatag tgcaataata aaccgttgtt acaaacgagt tacacgctat
     7681 aaatgggtgt aacgcttggt tgctgacttc ttattgacat gagtaatgcg taattgtttt
     7741 tgcttcacac tagttttaaa ttctgtatga cttaaataat atttagatga aaaatttgga
     7801 tagggagaaa ttatgcaagt tgaattaatt gttgccctca gtgccgctgc gttagcggtg
     7861 ctatacgggg ttgtcatgag taagtggatt actaatttgc ccgcaggaaa cgcgcgcatg
     7921 caagaaattg caggagcgat tcaacaaggg gcggcggctt acttggcccg ccaatataaa
     7981 accattgcaa tcgtcggtgt cattttagcg gtattaattg ccatatttct aggcaagcaa
     8041 actgccatcg gttttgtcgt aggtgccgtg ctttcaggcg catgtggctt tatcggcatg
     8101 aatgtctcag ttaaagcaaa cgttcgtacc gcacaggccg caacaggtgg catagccccc
     8161 gcatttgatg ttgcatttaa aggcggcgcc attactggca tgctggtggt tggtcttggc
     8221 ctattgggtg tcactgcttt ttattggtat ttaggtgctg accgtggcac tgatttaaat
     8281 ccactcattg gcttggcgtt tggttcttct cttatttcaa tttttgcacg tttaggcggc
     8341 ggcattttca ctaaaggtgc ggacgttggt gcggatttgg tgggtaaagt tgaagcgggc
     8401 atccctgaag atgacccacg caaccctgcc gtaattgccg acaacgtggg tgataatgtc
     8461 ggtgactgcg ctggtatggc ggcagactta tttgaaacct atgtcgtcac cattatcgca
     8521 actatggtgc ttggcggctt aatgctcgat aacgaaggca tcagcgcgat gatttacccc
     8581 ttaatgctgg gggcagtttc tattgttgcc tctatcattg gctgcttctt tgtcaaagca
     8641 tctcctggca tgaaaaacgt gatgccggcc ttatacagag gcttagcggt tgcagggcta
     8701 ctttcattgg ttgcatttta ttttgtcagc catcagttat ttccaaatgg ttttaaccat
     8761 gcaccaaacc ttgttgcttc agccaatgcc ttatttggtg cgtgcgcagt aggtttagtg
     8821 ttaactgcgg cactggtttg gattacggaa tattacacgg gcactgacta cgccccagtt
     8881 aaacatatcg ccaaagcctc aaccactggc catgcaacca acatcattgc aggcattggc
     8941 atttcaatga aatcaaccgc acttcctgtg atttcagtgt gtattgccat tttaacggcc
     9001 tatcatttag caggcttata tggcgtggcg attgcggcaa cttctatgtt gagcatggcc
     9061 ggcattatcg tggcgcttga tgcttatggc ccaattactg acaacgctgg cggcattgct
     9121 gaaatggtcg aattgccaag caacg
//
LOCUS       DAOQSI010000078         9126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_7031_length_9126_cov_5.322346, whole genome shotgun sequence.
ACCESSION   DAOQSI010000078 DAOQSI010000000
VERSION     DAOQSI010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 9126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9126
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7031_length_9126_cov_5.322346"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1624
                     /locus_tag="PKL42_06235"
     CDS             <1..1624
                     /locus_tag="PKL42_06235"
                     /inference="COORDINATES: protein motif:HMM:NF021572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2300 domain-containing protein"
                     /protein_id="HNU66712.1"
                     /translation="TAPQWWVGEAASAPAKVPIGSLWKLYVYNYSLALDLPDKPYQCH
                     AGRHAKVGDEYCCSQDETITRDIALARSCGAYFEPQRLRIDARQWQQYWQRNVPEIKW
                     LAQLENIQPQTQSSVHDILLSLNRLSANSIKHTRKALLGRILQPQWSVLLPHLGGAYR
                     FKTFTWVHPQYKGAYFGGAAGWLADGTAFWVGGTGSSKVVIEKAAPLIAEKLPIKGQS
                     QQSFDEECVAVHYFKRYPIQTVTRLSQRGADSTKVTSGALQGQYAVKFSNGNALQIIS
                     DGELSLTQRGNALQLWGKLSMQEYVARVLDREGDATKTEAAKALSIAARSYVYQNAHF
                     HLGCWQIDDDSRKQRVSPNPASVRASQVVGFTEDLSLVGSPIYFHQNKTKPNTLNWQH
                     AVNRAALGADYLALLHEAYPNASWRLNSQAQQCQRLPQAELYLRQSFPQAKQHLREVK
                     GVEVVQGLKVCQLDYGNPYADQHSTSIYVRDWRSENDRVTLWHEYLHLALRYHPNGHD
                     EALIEQNARRLASQLSLGKYTSSTKSKIRAAQ"
     gene            1656..2426
                     /locus_tag="PKL42_06240"
     CDS             1656..2426
                     /locus_tag="PKL42_06240"
                     /inference="COORDINATES: protein motif:HMM:NF021430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2135 domain-containing protein"
                     /protein_id="HNU66713.1"
                     /translation="MKNIGWYSIVLLTSVSFYGHAEEGQAPSRPMGGWRYSPSNQANF
                     SQTVNYPATRSNSGGHALSALIIGNIKARPKNSQNPHQLVVNGANMPLAVDESGHYER
                     PYAFGAGSNSVEVIDPQGKSVVKRQFYEANAGKAPAKLRIVLAWDSDHTDVDLHVVTP
                     NGEHAWYGQRTIPSGGSIDIDATDGFGPEIFATPNAIRGAYQVYANYYGGNSDQQVIT
                     RCKIAIISDENTPNEKQQNFIVPLRKAGELTLVKSFIY"
     gene            2581..4230
                     /locus_tag="PKL42_06245"
     CDS             2581..4230
                     /locus_tag="PKL42_06245"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HNU66714.1"
                     /translation="MQIQTIQTQAFNDQKPGTSGLRKKVKVFQQAHYLENFVQSIFDT
                     LREKEGIPAGAVLALGGDGRFHNREAIQTIISMAAANGFAKVMVGQAGILSTPAASHV
                     IRKYKTFGGLVLSASHNQGGVDGDFGIKYNMSNGGPAPEKITDSIFEKSKTISTYKMA
                     DLPQVNIDQIGETKFDGGFSVQVFDAVVDYADLMQEIFDFSAIKTLLASGFTMQFDAM
                     HAVTGPYAKEIFINRLGASASSLMNCEPSEDFGGGHPDPNLTYAEDLVKILFAGENSP
                     DFGAASDGDGDRNMILGKNFFVTPSDSLAVLAANATLIPAYKNGIAGVARSMPTSGAA
                     DRVAAKLNIPSYETPTGWKFFGNLMDAGKVTLCGEESFGTSSSHVREKDGLWAVLFWL
                     NILAKKGTSVEQVLMSHWAEFGRNVYSRHDYENIPTDAANSVIAHIKAQFTSLPNQKL
                     GSYTVKTCDDFSYHDPIDGSISNNQGIRILFDDGSRIVFRLSGTGTEGATIRIYLEAF
                     EPDTKNHHLDAQVALAEMIKIALQISLLKEKTGRDAPTVIT"
     gene            4270..4983
                     /locus_tag="PKL42_06250"
     CDS             4270..4983
                     /locus_tag="PKL42_06250"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNU66715.1"
                     /translation="MKKTVLLVTVFSVLVSIALSFLVATLIPSISVKVSLIMSVIVPL
                     IIAPLVSFPFVKLLFQLNALEVEMRALATYDALTKLLNRRAFLERADFAYNIAKREKQ
                     PFCILAVDLDFFKSINDQYGHATGDEVLAQFGLITNQISRESDICGRTGGEEFSFFLA
                     NTTIDEAENFTNRLHTAIRSASVNYKGLPIHFTVSIGLAAFTGYNISQLDALLQFADA
                     ALYEAKKSGRNQTVKSVGL"
     gene            complement(5018..5494)
                     /locus_tag="PKL42_06255"
     CDS             complement(5018..5494)
                     /locus_tag="PKL42_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HNU66716.1"
                     /translation="MSRHTLIFATIWIALAGVIYYIADGIQNPNKISKLGDASTVVLK
                     RGLDGHYRSEALINGQKVDVLVDTGATGVAISQRVADKLKLQSIDAVRTNTANGNSVG
                     YMVRLKSIQIGGVHAENVAAMIAPGLDGDVLLGMSFLGRMDVRLYKGEMTIKQVHE"
     gene            complement(5499..5876)
                     /locus_tag="PKL42_06260"
     CDS             complement(5499..5876)
                     /locus_tag="PKL42_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HNU66717.1"
                     /translation="MPEVAFIALIWLFVKHFICDFPLQATPWMYRNKGTYGHAGGLAH
                     SALHGIGTFVILFYWLGLHAWVYAMAETMIHYHIDWAKMNTNRYFNLKPDSSEWFWIS
                     LGFDQLLHHLTYFGIVAFAFKLL"
     gene            complement(5882..6076)
                     /gene="iscX"
                     /locus_tag="PKL42_06265"
     CDS             complement(5882..6076)
                     /gene="iscX"
                     /locus_tag="PKL42_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HNU66718.1"
                     /translation="MKWTDTSTIAEMLYDAHPDIDPKTIRFTDLMQWVLALEGFNDLP
                     EHCGEKILEAIQLAWIDEAA"
     gene            6481..7752
                     /gene="glyA"
                     /locus_tag="PKL42_06270"
     CDS             6481..7752
                     /gene="glyA"
                     /locus_tag="PKL42_06270"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNU66719.1"
                     /translation="MTSTTIHPAPFHFANTLNQVDPALDAMIKGEVVRQLEHIELIAS
                     ENYTSPAVMQAQGSQLTNKYAEGYPGKRFYGGCEYVDQVEQLAIDRLKALYGAEYANV
                     QPHSGSQANQAVYFSILKPGDTVMGMNLGHGGHLTHGSPANLSGKLFNIVPYGLNANE
                     EIDYDEMERIAVECKPKLLIGGASAYSLRFDWARMAEIAKKVGAYFMVDMAHYSGLIA
                     AGVYPNPVPHADFVTSTTHKTLRGPRGGIILAKAEFEKSLNSSVFPALQGGPLMHVIA
                     AKATAFLEASQPEFKAYQAQVINNAQAMAQALTARGLRIISGRTESHMFLVDLRPKGL
                     TGKAADAALGLAHITVNKNSIPNDPESPFVTSGIRIGAPAITTRGFKEDEAKLVANLI
                     ADVLDNPADETVIAAVKAKVHALTARFPVYQ"
     gene            7864..8307
                     /gene="nrdR"
                     /locus_tag="PKL42_06275"
     CDS             7864..8307
                     /gene="nrdR"
                     /locus_tag="PKL42_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNU66720.1"
                     /translation="MKCPFCGAVDTQVIDSRVNDEGDSIRRRRKCGACDKRFTTYETA
                     ELHMPQVVKTNGTREEFNREKLRLSFSRALHKRPVPTEYVDRALDKISQKLLSLGVRE
                     IPARDLGESVMHELKLMDKVAYIRFASVYRSFSDVDDFNDAIRDL"
     gene            8356..8733
                     /locus_tag="PKL42_06280"
     CDS             8356..8733
                     /locus_tag="PKL42_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66721.1"
                     /translation="MKLINWLTIGFAVFTLILTIVFFGWWTMKDSVVFGSEQFDQVKW
                     MQHAATIEQECKRGDMAYDLQQNVLSKGTAREAVSVLLGRPSYEDEKSMEYDLGKCMH
                     VYHGLLLFFDKNNRLINSRISSH"
     gene            8794..>9126
                     /gene="ribD"
                     /locus_tag="PKL42_06285"
     CDS             8794..>9126
                     /gene="ribD"
                     /locus_tag="PKL42_06285"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNU66722.1"
                     /translation="MTLALQLAEQGLYTTTPNPRVGCVIVKNNRIIGQGAHLKAGEPH
                     AEVLALRTAGEHAKGADVYVTLEPCNHYGRTPPCVDALIQAVVGRVVVAMQDPNPLVA
                     GNGVKRLQA"
BASE COUNT         2534 a         1918 c         2240 g         2434 t
ORIGIN      
        1 gactgcccca caatggtggg tgggtgaggc ggctagtgct ccagcaaaag tgccaattgg
       61 aagcttatgg aaactttatg tctacaacta tagcttggct ctcgacttgc ctgacaaacc
      121 gtaccagtgc catgctggcc ggcatgcaaa agttggtgat gaatattgtt gtagtcaaga
      181 cgaaactatc acgcgagata ttgcgctcgc ccgctcatgc ggcgcttatt ttgaacctca
      241 gcgtttgcgt atagatgcaa ggcaatggca gcaatattgg cagagaaatg tgccagaaat
      301 taaatggtta gcccagcttg aaaatataca gccacaaaca caaagtagtg tgcatgatat
      361 tttgttgagt ttaaaccgcc tgtctgccaa tagcattaag cacactcgca aagcactatt
      421 aggacggata cttcagccgc aatggagtgt actgttgccg catttaggcg gcgcgtatcg
      481 cttcaaaaca tttacttggg tacatccaca atacaaaggg gcttactttg gtggtgctgc
      541 agggtggttg gcagatggta cggctttttg ggttgggggt acaggaagca gtaaagtcgt
      601 tatagagaaa gcggcgccac taattgctga aaaattgcct ataaaagggc agtctcagca
      661 aagttttgat gaagagtgtg tggcggttca ttattttaag cgctacccca ttcaaacggt
      721 tacgcggtta agtcagcgtg gtgctgactc cacaaaggtg acaagcggcg cattgcaagg
      781 ccaatatgca gttaaattta gtaacggcaa tgcgctgcaa ataataagtg acggtgagtt
      841 aagcttaacg cagcgtggta atgctctgca gttatggggg aaattaagca tgcaagagta
      901 tgttgcacgc gtgcttgacc gtgaagggga tgcgacaaaa acagaggcag caaaagcgct
      961 gagtattgcc gcgcgtagtt acgtttatca aaacgcgcat tttcatctgg gctgttggca
     1021 gattgatgat gatagccgta agcagcgggt ttcaccaaac ccggcgagcg tcagggcaag
     1081 tcaggtggta gggtttactg aagacttaag tttagtgggt agcccgattt attttcatca
     1141 aaataagaca aaaccaaata cgctaaattg gcagcatgct gtgaatcgtg cggcacttgg
     1201 cgctgattat ttagcattgc tacatgaagc ctaccccaat gccagctggc ggctgaatag
     1261 tcaagctcaa cagtgccagc gtttgccaca ggccgaactt tatttgcggc agagttttcc
     1321 acaagcaaag caacatttgc gcgaagtaaa aggggttgaa gtggtacaag gattaaaagt
     1381 ttgtcagcta gactatggta atccgtatgc agatcagcat agtacgagca tttatgtgcg
     1441 cgattggcga agtgaaaatg accgggtgac gttgtggcat gagtatttac atttagcact
     1501 tcgttatcat ccgaatgggc atgatgaagc cttgattgag caaaacgcac gacgactggc
     1561 gagtcaatta tcgctgggaa aatatacaag tagcactaaa agtaagatac gtgctgcgca
     1621 atagtaaatg tgttagaaat actttaggta aacatatgaa aaatataggt tggtattcaa
     1681 tagtgttatt aaccagtgtg agcttttacg gccatgctga agaaggccaa gcgccaagtc
     1741 gtccaatggg ggggtggcgt tatagtccgt caaatcaagc gaatttttcg caaacagtca
     1801 attaccccgc cacacgcagt aacagtggcg gtcacgcttt gagcgcgctg attatcggca
     1861 acataaaagc acgtccaaaa aacagccaaa atccgcatca acttgtggtg aacggtgcca
     1921 atatgccgct cgcggtagat gaatctggcc attacgagcg accttatgcc tttggcgcgg
     1981 gcagcaacag tgtagaagtt atcgacccac agggtaaaag cgtcgtaaaa cgccaatttt
     2041 atgaagccaa tgcaggtaaa gcgcctgcaa aattaagaat tgtgttggca tgggattctg
     2101 atcataccga tgtggatttg catgtggtca cgccaaatgg tgagcatgct tggtatggac
     2161 aacgcacgat tccgagtggc ggctcgattg atattgatgc caccgatggg tttggcccag
     2221 agatttttgc aaccccaaac gcaatacgtg gcgcgtatca agtgtatgcg aattattacg
     2281 gcgggaatag cgaccaacaa gtcattacgc gctgtaaaat tgccattatc agtgatgaaa
     2341 atacacccaa tgaaaaacag caaaacttta ttgttccgct acgaaaagcg ggcgagttga
     2401 cgttggtgaa gagttttatt tattgaaatt ttgcacttat aatttaccga gcaaaaatca
     2461 ctgccctgca agccttgtaa attaaggctt gcagggcagc tgtaatatag acatgcgaat
     2521 ttaatcaata cagcagtata attaacgtta ttctagtgac gattttccaa aaggcatttt
     2581 atgcaaattc aaacgattca aacccaagct tttaatgacc aaaaacctgg cacttctggc
     2641 ttacgtaaaa aggttaaagt gtttcagcag gcgcattatt tagaaaattt tgtgcaaagt
     2701 atttttgata ccctacgtga aaaagagggc attcctgcgg gggcagtttt agcgttggga
     2761 ggcgatggtc gttttcacaa tcgcgaggcg attcaaacca ttattagcat ggcggcggcc
     2821 aatggttttg caaaggtaat ggtagggcag gctggtattt tatcaacgcc tgcggcttca
     2881 catgtgattc gcaaatataa aacatttggt ggtttggtgc tgtctgccag ccataatcaa
     2941 ggcggtgtgg atggcgattt tggcattaaa tataatatga gcaatggtgg gcctgcgcct
     3001 gaaaaaatca ccgatagtat ttttgaaaaa agcaaaacga ttagcactta taaaatggca
     3061 gatttgccac aagtgaacat tgaccaaatt ggcgaaacaa aatttgacgg cggctttagt
     3121 gtgcaagtgt ttgatgcggt ggtggattat gcggatttaa tgcaagaaat ttttgatttt
     3181 tctgctatca aaacgttatt agccagcggc tttaccatgc agtttgatgc catgcacgcc
     3241 gtgactggcc cttatgccaa agaaattttt atcaatcgct taggcgcctc tgccagcagc
     3301 ttgatgaact gtgaaccctc agaagatttt ggtggcggtc acccagatcc gaacttaact
     3361 tatgccgaag atttagtcaa aatcctattt gcgggtgaaa actcacctga ttttggcgcg
     3421 gcatccgatg gcgatggcga ccgcaatatg attttgggca aaaacttttt tgtgacacct
     3481 tctgacagtt tggcggtgtt ggcagcgaat gcaacgttaa tccccgctta caaaaatggt
     3541 attgcaggcg tggcgcgctc gatgcccacc agtggcgcgg ccgaccgcgt agccgcaaaa
     3601 ttaaatatcc ccagttatga aacacccaca ggctggaaat tttttggaaa cttgatggac
     3661 gcgggcaaag taacgctttg tggtgaagaa agttttggca ccagttctag ccacgtgcgc
     3721 gaaaaagatg ggctttgggc ggtgctgttt tggctgaata ttttagccaa aaaaggcact
     3781 tcagttgagc aagtgttgat gtcacactgg gcagaatttg gccgcaatgt gtattcacgt
     3841 catgattatg agaatattcc aacagatgct gcgaatagcg tgattgccca tatcaaagcg
     3901 caatttacca gcttgcccaa ccaaaagctg ggttcatata ctgtgaagac ctgcgatgat
     3961 tttagttacc acgacccaat tgacggctca ataagcaata accaaggtat tcgtatttta
     4021 tttgacgatg gctcacgcat tgtgtttagg ctttcaggca cgggtactga gggcgcgacg
     4081 attcgtattt acctagaagc gtttgagcct gacactaaaa accaccattt agatgcgcaa
     4141 gtggcgctgg ctgagatgat taaaattgcc ttgcaaattt cgctactgaa agaaaaaacc
     4201 ggacgcgatg ccccaacagt gattacttaa aataagcgcc gagtgaagca tttcatttta
     4261 aaggtaggca tgaaaaaaac ggtgttattg gtcactgttt tttctgtttt agtgtcaatc
     4321 gcactgtctt ttttggttgc aacattgatt ccttctattt cagtgaaagt ctcgctgata
     4381 atgtcggtaa ttgtgccgct tatcatcgcg cctttagtga gctttccttt tgttaaatta
     4441 ttgtttcaat taaatgcact tgaagtggaa atgcgagcgc tggccactta cgatgcgctt
     4501 accaagttgc tcaatcggcg tgcattttta gagcgtgcag actttgctta caatattgca
     4561 aagcgagaaa agcaaccttt ttgtattttg gcggtagatt tagatttttt taagtctatt
     4621 aatgaccagt acggccatgc aacaggggat gaagtgctgg cgcaatttgg tctaatcacc
     4681 aatcaaattt cgcgagagag cgatatctgt ggccgaaccg ggggcgaaga gttttcattt
     4741 ttcttagcca ataccacgat tgatgaagcc gagaatttta ccaatcgctt gcatacagcg
     4801 ataaggtcag cttcggtgaa ttataaaggc ttaccaattc actttaccgt gagtatcggg
     4861 ctggccgctt ttactggtta taacatcagt caattagatg cattgttgca atttgctgat
     4921 gccgctttgt atgaggcgaa aaaaagtggt cgaaatcaaa cggttaaatc agtagggttg
     4981 tagtcaatgt agcaaggtgg ctgataaatg agccttttta ctcatgaact tgcttaatcg
     5041 tcatttcgcc tttataaagc cgcacatcca tacggcctaa aaaagacatg cctagcagca
     5101 catcaccatc taagccgggg gcaatcatcg cggcaacatt ttcagcatgt acgccaccaa
     5161 tttgtattga tttcaagcgc accatataac ccacgctgtt gccattggcg gtattggtgc
     5221 gaacagcatc aatactttgt agtttgagtt tatctgccac gcgttgcgat atggccacgc
     5281 cagtcgcgcc ggtatctact aatacatcga ctttttgtcc gttgatgagc gcttcacttc
     5341 ggtagtggcc gtctaagcct cgtttaagta ctacggtgct ggcatcgccc agcttgctta
     5401 ttttgttggg gttttgaatg ccatcggcga tgtaataaat gacgcccgcc agcgcaatcc
     5461 aaatggtggc aaatattagc gtgtgacgag acatggcgct atagcaactt aaacgcgaaa
     5521 gccacaatgc cgaaataggt gaggtggtgc agcagttgat caaaccctaa cgaaatccag
     5581 aaccattcgc tgctatcggg cttgaggttg aaatagcggt tggtattcat tttagcccag
     5641 tcaatgtggt agtgaatcat agtctcagcc attgcataaa cccaggcatg caatcccagc
     5701 cagtaaaata gtatgacaaa agtgccaatg ccatgcaggg ccgaatgtgc caagccgcct
     5761 gcatgcccat aagtgccttt gttgcgatac atccaagggg tggcctgtag tggaaagtcg
     5821 caaataaaat gcttaacaaa taaccatatt aaggcgatga atgctacttc aggcatgtta
     5881 attacgctgc ttcatctatc caagcaagtt gaatggcttc tagaattttt tcaccgcaat
     5941 gttcgggcaa atcattaaaa ccttccagcg ccaacaccca ttgcatcaag tcggtaaaac
     6001 gaatggtttt tgggtcaata tcagggtgcg cgtcataaag catttcggca atggtgcttg
     6061 tgtctgtcca tttcatcggg agatttcctt acaggtggtt gaaccttatt ataacgctgt
     6121 gacctaaacg cggcaaagtg attcaattgc gcgtaagttt tgagaattgg ttagcactca
     6181 atgattttaa ccctgtcaaa ttctgccaat aacttgggtt gaaaaaacat cttttttact
     6241 tgcatttgcg tgcgagaaaa aatcgctgcc ctgcgagcct ttatttataa ggctcgcagg
     6301 gcgggtcaaa agggttgctg taaaataatg gaaaaaacac gtgaattttg ctttgcaaca
     6361 acttgtgaaa ataggtgccg aactgggcgc cactggtttt attttttaac ctttaatctt
     6421 caacctagag cgtggtaaaa tgccgcatca atttattcaa gtatttggaa aacttttctt
     6481 atgacttcta caaccataca tcccgcccca ttccattttg ctaacacctt aaaccaagtg
     6541 gatccagcct tagacgccat gattaaaggt gaagtggtgc gccagcttga gcatattgag
     6601 ctgattgctt cagaaaacta caccagccca gcggtgatgc aggcgcaagg ttcgcagctc
     6661 accaataaat atgctgaagg ctatcctggc aaacgttttt acggtggctg tgaatatgta
     6721 gaccaagttg agcagttggc gatagaccgc ttaaaagcat tgtatggcgc agagtacgcg
     6781 aacgtgcagc cgcattctgg ctcgcaagcg aatcaagccg tatatttttc aatattgaaa
     6841 cctggcgata cagtcatggg catgaattta ggtcatggtg ggcatttaac gcacggatca
     6901 ccggctaatt tatcaggtaa attattcaac attgtgcctt atggcttgaa cgccaatgaa
     6961 gaaatcgatt acgacgaaat ggagcgcatt gcggtggaat gcaagccaaa attgttaatc
     7021 ggcggtgcat cggcatattc gctacgtttt gattgggcgc gtatggcgga aattgccaaa
     7081 aaagtgggcg cctattttat ggtggacatg gcgcattact caggcctcat tgcagcgggc
     7141 gtttatccaa accctgtgcc gcatgctgat tttgtaactt caaccaccca caaaactttg
     7201 cgcggcccac gtggcggcat tattttggcc aaagcagaat ttgaaaaatc actcaactcc
     7261 agcgtgttcc ctgcattgca aggcggtcct ttaatgcatg tgattgcggc aaaagccacg
     7321 gcatttttag aagcctcaca gcctgaattt aaagcttatc aagcacaagt gattaacaat
     7381 gcccaagcaa tggcacaagc gttaacagcg cgcggtttac gaattatttc aggccgtaca
     7441 gaatcgcaca tgttcttggt ggatttacga ccaaaagggt taaccggcaa agcggctgat
     7501 gcggcacttg gtttggcgca cattaccgtg aacaaaaact cgattccaaa cgaccctgaa
     7561 agcccgtttg ttacttctgg tatccgcatt ggcgcgccag ccattaccac gcgcggcttt
     7621 aaagaagatg aagcaaaatt ggtggcgaat ttgattgcgg atgtgttgga taatccagca
     7681 gatgaaacag tcattgcggc agttaaagct aaagtacatg ctttgacggc gcgttttcct
     7741 gtgtatcaat aaatctgtga aaagtgaaat gagcaacgtg aaaagtaaaa acgccacatt
     7801 gcaaaatgtg ccgattggtt tttgtttctc cattcacctt tcacctttta ccggttcacc
     7861 atcatgaaat gccccttctg cggcgcagtt gatacgcaag tgattgattc acgcgtgaac
     7921 gacgaaggcg attcgattcg ccgtcggcgc aagtgcgggg cttgcgacaa acggtttacc
     7981 acctatgaaa ccgctgaatt gcacatgccg caagtggtaa aaaccaatgg tacgcgtgaa
     8041 gaattcaacc gtgaaaaatt acgtttgtct ttttcacgtg ccttgcataa acgccccgtg
     8101 ccgactgaat atgttgaccg cgccctcgat aaaatctcgc aaaaattgct aagtttgggc
     8161 gtgcgagaaa tccccgcacg cgatttaggc gagagcgtga tgcatgaact taaactcatg
     8221 gataaagtcg cttatattcg ctttgcatcg gtgtatcgca gcttttcgga tgtggacgat
     8281 tttaacgatg cgattcgtga tttgtaacac attcaataat catcaataac aggctcacgt
     8341 taaaggaaat taggcatgaa gttaatcaac tggcttacga ttggttttgc ggtgtttacg
     8401 ctcatattaa ccattgtgtt ttttggttgg tggacaatga aagacagtgt ggtgtttggc
     8461 agtgaacagt ttgatcaagt gaaatggatg cagcatgcgg ccaccattga gcaagaatgc
     8521 aagcgtggcg acatggccta tgacttacag caaaatgtat tgtcaaaagg taccgcgcgt
     8581 gaagcggtga gcgtgttatt gggtcgccca agctatgaag atgaaaaatc catggagtac
     8641 gatttaggta aatgcatgca tgtttaccac ggcttactgc tgttttttga taaaaataac
     8701 cgtttaatca acagccgaat ttcttcacac taatcgcaca ccccatgcgc gaatggtttt
     8761 acacttttgt tctccgccgc tgaccatagt tacatgacct tagcgctgca attggcagag
     8821 caaggtttgt acacgacaac acccaacccg cgtgtgggtt gtgtcattgt taaaaataat
     8881 cgaatcatcg gccagggcgc acatctcaaa gcaggcgagc cgcatgccga agtgctggct
     8941 ttgcgcacgg ctggggagca tgcaaaaggt gccgatgtat atgtaacgct tgagccttgc
     9001 aaccattatg gccgaacgcc accctgtgtg gatgctttaa ttcaagcagt ggtggggcgc
     9061 gttgtggtgg cgatgcagga ccctaatcca ttggtggcgg gcaatggcgt taagcggttg
     9121 caggct
//
LOCUS       DAOQSI010000079         9044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_7122_length_9044_cov_6.519301, whole genome shotgun sequence.
ACCESSION   DAOQSI010000079 DAOQSI010000000
VERSION     DAOQSI010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 9044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9044
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7122_length_9044_cov_6.519301"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..1385)
                     /locus_tag="PKL42_06290"
     CDS             complement(117..1385)
                     /locus_tag="PKL42_06290"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HNU66723.1"
                     /translation="MMQKMSTSAAPAAAISKMNGFENKANASAEMQTVAPASTFQSVL
                     KSQVQTRQPKPTAEAAKQHTPHTAKQVAAKTANQTDAGQKTNHTTAVISNIARQDEAE
                     PIATSKAVDEMVELAGMALPAEAKSVNQDEALTADPSKNSQPEGIALFGLMPTVTKLT
                     PLQDNAQDVQVTINTQTASSDAKNDSSIELGKPDVIAPKDASIANSVTRAGVSPQFNE
                     VKPLQTQPQDLVLNAANANQDVETPMPLASAISGLQKSLATPSIESPQIGQQVAQQVA
                     TSHVAEAFSGKATWNQAISQRVLWMVGAGEQSATLTLNPPDLGPLQVVVHVHNGMADT
                     RFTSDNAEVRQALQDGMDNLREKMRESGVQLGQTNVQSGEQSRQEFQQTSQQNNLGAR
                     NSTTTEATQNASVMSQVKSYVANGLVDTFA"
     gene            complement(1448..1891)
                     /gene="fliJ"
                     /locus_tag="PKL42_06295"
     CDS             complement(1448..1891)
                     /gene="fliJ"
                     /locus_tag="PKL42_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HNU66724.1"
                     /translation="MTTISPQVLKLLTETAVKEVELATEALAKAMKQATEAQQKLDML
                     IEYRRDYLNNLSKTLERGMSAEAHQNFHHFLAKLDQAVAGQKDVLVSVQYHVTVQKQL
                     WQECQRKKLSYEVLATRKNKQLHHVALKKEQKEMDEFAMRASHKK"
     gene            complement(1903..3336)
                     /gene="fliI"
                     /locus_tag="PKL42_06300"
     CDS             complement(1903..3336)
                     /gene="fliI"
                     /locus_tag="PKL42_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HNU66725.1"
                     /translation="MQASAISQEKAIGNLHQERWQDYMRDCREILRIIEPLEIAGRIT
                     KLTGLVMEAVGIQLPIGCACYVPLAEGRRVEAEVVGFDGDVLLLMPQSSVDGVVPGAK
                     VFALEIAEALPKPHFNIPPRRRVTDRARHLPVGEDLLGRVVDGAGNPLDNLGKIASSK
                     SAPLSARPINPLMRAPIEETLDVGVRAINAMLTVGRGQRMGLFAGSGVGKSVLLGMMA
                     RYTTADVIVVGLIGERGREVQEFIENILGEEGLARSVVVAAPADASALMRLQGANYAT
                     TIAESFRNQGKNVLLIMDSLTRYAMAQREIALAIGEPPATKGYPPSVFAKLPALVERT
                     GNGMRGEGSITAFYTVLTEGDDQQDPIADAARAILDGHIVLSRSLAESGHYPAIDIEQ
                     SISRAMHNITSPAHQKLARKLKQLNSRYMRSIDLINVGAYEAGSDQVLDEAIAKHEMV
                     EAFLQQDIDESAGFGDSLQGLAAILEV"
     gene            complement(3336..3992)
                     /locus_tag="PKL42_06305"
     CDS             complement(3336..3992)
                     /locus_tag="PKL42_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HNU66726.1"
                     /translation="MSNFATPKEQQTAYERWEMSSFPDDFSQSRPARQKPEKSTTDAG
                     VLAKIIEGARKEAYEKGIQEGFTVGMAKAREQALVEKQAFAQLMDSFSTALENKDAQI
                     AEDVLCLALDIAKSMLKIKLNVDPTVILPLVMDAIHYLPSVQKPARIMVHHDDAHILR
                     EYLAEELLSQEWQVQEDSNIERGGCLVETGANQIDATTQVRWKRISEALAQKNDWLLP
                     "
     gene            complement(3985..4983)
                     /gene="fliG"
                     /locus_tag="PKL42_06310"
     CDS             complement(3985..4983)
                     /gene="fliG"
                     /locus_tag="PKL42_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HNU66727.1"
                     /translation="MSDAGVMRSAVLMLALGEDEAAEVMKYLGPKEVQKLGAAMASLK
                     SVGRDQVETALSEFMLEAEQNSNFGLDSDEYIRSVLTKALGDDKASNLLNRILQSSDA
                     SGIEGLKWMDAPSVAELIKNEHPQIIATILVHLEADQAAEIVSNFTDRLRQDVMLRIA
                     TLDGVKPIALRELNEVMTKLLTGNENLKKQSMGGIKVAAEIMNFMGGEKEAELMEGLK
                     SYDEDMAQQIMDEMFVFDNIMDVDDKGIQALLREVQSETLIIALKGTTEEMRDKIFRN
                     MSSRAAEMMKEDLETKGPVKLSEVEAQQKQILQIVRRLVDEGQIMLAGKGGDDQYV"
     gene            complement(4984..6630)
                     /gene="fliF"
                     /locus_tag="PKL42_06315"
     CDS             complement(4984..6630)
                     /gene="fliF"
                     /locus_tag="PKL42_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HNU66728.1"
                     /translation="MAASNATLTNESAMGMGGMPQLGNKLMLVAGMAAVVAVMVVFWL
                     WSQQPDYRVLYSNFSDKDGGAIVAALEKMNVPYKFSEGGSAILVPAGQVHQARLKLAA
                     DGLPKGGNAGFELLENQKFGVSQFVEQVNFQRALEGELERSVQSISAVEGAKIHLAIP
                     KATVFVRDQLKPTASVLLNVRAGRNLDPQQVAAVVHLVASSVPNLPIENVTVVDQHGT
                     LLSDTSKKAGANGLDATQIKYIDEMQQSIVKRVESIIAPIVGAKNVRAESNVEIDFSS
                     TEQAAETYKPNQRTEDAAIRSMQNSESQTGNGGAATGVPGALSNQPPANATAPIVADG
                     ATAGAAAGAEATAINSQKNTTTNFEVDKTVRYTQQTMGGVKRINVAVVVNNMQVIDAK
                     GKVTYRPLSAAEKQQINDLAMQAMGFNKERGDSLTIVNSPFAENPVEPVAELPLWKNP
                     TVIEYGKDALRFLVGIIVLMMLYKRALKPMLNKLTTASPQLLPAPQGGQEDATVTLSN
                     QSGNAVPPYEQNLLAAKQMAKDNPRIVASVVSNWTNGNES"
     gene            6902..7261
                     /gene="fliE"
                     /locus_tag="PKL42_06320"
     CDS             6902..7261
                     /gene="fliE"
                     /locus_tag="PKL42_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HNU66729.1"
                     /translation="MKTSGIDAGRIESMLAQLKAAAQRPQAEALTALNVSGTKSAGNA
                     SKVDFSNALKASLDQVNQVQKSAEQLGKNFAMGDDSVSLSDVMIANQKANISFQATIQ
                     VRNKLVSAYHDIMNMQV"
     gene            complement(7374..7700)
                     /locus_tag="PKL42_06325"
     CDS             complement(7374..7700)
                     /locus_tag="PKL42_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HNU66730.1"
                     /translation="MNNLAQPHHHQQAIALAYGANDYAPKVVAKGHGAVAEQIIALAK
                     EHKVFVHESKDLVNLLMQVDLDAHIPPELYLAIAEILAWIYRLEAEKAQSQQAEKFEE
                     KIENWR"
     gene            complement(7693..8652)
                     /locus_tag="PKL42_06330"
     CDS             complement(7693..8652)
                     /locus_tag="PKL42_06330"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HNU66731.1"
                     /translation="MFKPADLTSILPVPKVLPIVAVEKIGDPAQALLSRATQFLVGQE
                     YLATVAAKTTDGKQLVEIEGQLLKMDLGSQVDKGQTLSLRFMQSQPVPTFYLLSADKF
                     NSAATAAISQTGFLLGSIIKQANEEGTSMRYQANGVVTQQPYNPQQLGLDLKNALQRS
                     GLFLESHLAEVVAGKRTIESMRPELQNLANNTPNTVLNTLPAQQLAALEHQRLSWHGE
                     IWQNQTMDWDVTFDEPREHAEQSSQDETQKTKPVSSEITMHLPNLGKVSARIGVVDGR
                     ISIHMLTENPQTLATFKKERQQLFASMVNKGQAIEALTVGQYE"
     gene            complement(8680..9003)
                     /locus_tag="PKL42_06335"
     CDS             complement(8680..9003)
                     /locus_tag="PKL42_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HNU66732.1"
                     /translation="MEQQNTVVLYESVAGIMHRMLDAAKQQEWDALAEMESHCAQMVQ
                     MIKVMESSSKLSEEELARKLSSVKSILANDREIRNIVSPWMAKLNAMMHSNHMEQKLS
                     QAYQH"
BASE COUNT         2154 a         2277 c         1933 g         2680 t
ORIGIN      
        1 ctctgcgagc cttataaata aaggctcgca gagcataaaa tttttctcgg gagaattaaa
       61 ccaatgtaac acctaaaaat tgagtgtaga ttttcgcctt aaatgagtgc ggttggctat
      121 gcgaaagtat ctaccaagcc gttggcaaca tacgatttca cttgactcat cacggatgca
      181 ttttgcgtgg cttcagtggt ggtactgtta cgtgcgccta aattgttttg ctgtgaagtt
      241 tgctgaaact cctggcgtga ttgctcaccc gattgcacat tggtttggcc taattgcaca
      301 cctgattcgc gcattttctc gcgaaggtta tccataccat cttgcagtgc ttgtcgcact
      361 tcagcattat ccgaagtaaa ccgcgtatcg gccatgccat tatgcacatg caccaccact
      421 tgcagcggcc ctaaatctgg cgggttcagg gttaacgtgg ctgactgctc gcccgccccc
      481 accatccaaa gcactctctg gctaatggct tgattccaag tagctttgcc tgaaaaagct
      541 tcagccacat ggcttgttgc aacttgctgc gcgacttgtt ggccaatttg cggactttca
      601 atagaaggcg ttgccaatga tttttgtaac cctgaaatgg ctgaggccaa tggcattggg
      661 gtttctacat cttggttagc atttgcagcg ttaagtacaa ggtcttgcgg ctgcgtttgc
      721 agtggtttaa cctcattgaa ttgtggagaa acccctgcac gtgtaactga atttgctatt
      781 gaagcatctt ttggcgcaat aacatctggt ttgccaagtt caatcgacga gtcatttttg
      841 gcatcactgc tagccgtttg tgtattgatt gtcacttgta catcttgggc attgtcttgc
      901 aatggggtca attttgtcac cgtaggcatc aatccaaaca atgcaatacc ttcaggttgg
      961 ctgtttttac tagggtcggc ggtcagtgct tcatcttgat taactgactt ggcttcagca
     1021 ggcaaggcca tgccagccaa ttccaccatc tcgtccaccg cttttgaagt cgctataggt
     1081 tcggcttcgt cttgccgcgc gatattgctt ataacagccg tagtgtgatt tgttttttgg
     1141 ccagcgtctg tttgattcgc tgttttggcg gccacctgct ttgcagtgtg cggcgtgtgt
     1201 tgtttggctg cttcggctgt tggctttggc tggcgcgttt gcacctgcga tttcaatacc
     1261 gattggaatg tactggcagg ggctacggtt tgcatttcag cactggcatt ggctttattc
     1321 tcaaagccgt tcattttgct gattgcggca gctggcgctg ctgacgttga cattttttgc
     1381 atcataccaa actccaaatt atgacctaat ttcgtttagg gtaaataaac cgcttaaatc
     1441 ttccacgcta ttttttatgg cttgcccgca tggcaaactc atccatctct ttttgctctt
     1501 ttttaagcgc cacatgatgc aattgcttat ttttacgtgt ggcaagcact tcataagaga
     1561 gctttttacg ctgacattct tgccaaagct gtttttgcac cgtgacgtga tattgcactg
     1621 acaccagcac atctttttgc ccggccaccg cttggtctaa ctttgccaaa aagtgatgga
     1681 aattttggtg cgcttcagcg ctcatgccgc gctcaagggt tttactcaaa ttatttaagt
     1741 aatcacgccg atattcaatc agcatgtcga gtttttgttg tgcctctgtg gcctgcttca
     1801 tggcctttgc caaagcttcg gtggcaagtt ccacttcttt tactgctgtt tcagtaagca
     1861 gttttaatac ttgaggagaa attgtcgtca tcgggcgcta ctctatacct ctaaaatggc
     1921 agctaagccc tgcaaactat cgccaaagcc tgctgactca tcaatatctt gctgtaaaaa
     1981 agcttcaacc atttcgtgtt ttgcaatcgc ttcatccagc acttggtcac tgcctgcttc
     2041 ataagcgccc acattaatta aatcaatgct gcgcatataa cgcgaattaa gttgttttaa
     2101 tttacgtgct aatttttgat gtgctgggct ggtaatgttg tgcatggcac ggctaatcga
     2161 ttgttcaata tcaatcgcag gataatgccc gctttcagcc aagctgcggc taagcacaat
     2221 gtggccatct aaaatcgcac gtgccgcatc ggcaatgggg tcttgctggt cgtcgccttc
     2281 agtcagcaca gtataaaaag cggttattga accttcgccg cgcatgccgt taccggtgcg
     2341 ttccaccaac gcgggcaatt ttgcaaaaac agagggcgga taacctttcg tcgcaggcgg
     2401 ctcaccaatc gccaacgcaa tttcacgctg cgccatggca taacgggtga gtgaatccat
     2461 aatcagcaac acatttttgc cttggttacg aaagctctcg gcaatggtgg tggcgtaatt
     2521 agcaccttgc agacgcatta acgctgaagc atctgccggc gcggcgacta ccactgagcg
     2581 cgccaagcct tcttcaccca aaatgttttc aataaactct tgtacttcac gaccacgctc
     2641 gccaattaaa ccgaccacaa tcacatccgc agtggtgtaa cgggccatca tgcctagcag
     2701 cacactttta cccacgccag aaccagcaaa taagcccatc cgctgcccac ggcccacagt
     2761 cagcatggcg ttaattgcgc gtacacctac atccagcgtt tcttcaatcg gcgcgcgcat
     2821 cagcggatta atcgggcgcg cactcaaggg cgcacttttg cttgatgcaa ttttgcctaa
     2881 attatctaac gggttgcccg caccatcgac cacgcggcct aataaatctt cacctactgg
     2941 caagtggcgg gcgcgatcgg tgactcttct gcgtggcggg atattaaaat gtggctttgg
     3001 tagcgcttct gcaatttcaa gtgcaaaaac ttttgcccct ggcaccacac catctacact
     3061 gctttgcggc atgagcaata gcacatcgcc atcaaaccct accacttcag cctccacacg
     3121 gcggccttca gctagcggca cgtagcaggc acagccaatc ggtaattgaa tgccaacagc
     3181 ttccatcacc aagccggtga gttttgtgat acggccagca atttcaagtg gctcgataat
     3241 gcgtaaaatt tcacggcaat cgcgcatgta atcctgccag cgctcctgat ggaggttgcc
     3301 gattgctttt tcttggctga tagcgctcgc ttgcattatg gcaataacca atcatttttt
     3361 tgcgccaaag cctcactaat tcgcttccaa cgcacttgtg tagtggcatc aatttgattc
     3421 gccccagttt caaccaaaca accgccgcgc tcgatattgc tatcttcttg cacttgccat
     3481 tcttggctca ataactcttc ggccagatac tcgcgcagaa tgtgcgcatc atcatgatgc
     3541 accataattc tggctggctt ttgaacactt ggtaagtaat gaatcgcatc catcaccaat
     3601 ggcaaaatca cagttgggtc tacgttgagt ttaattttta gcatcgattt tgcaatgtct
     3661 agcgcaaggc atagtacatc ttcagcaatt tgtgcatctt tattttctaa cgctgtacta
     3721 aaactatcca tcaactgggc aaacgcctgc ttttcaacta aagcctgctc tctggcctta
     3781 gccatgccaa ctgtaaagcc ttcttgaatg cctttttcat aggcttcttt gcgcgcgcct
     3841 tcaataattt tggctaacac acccgcgtct gtcgtgcttt tttctggctt ttgtctggct
     3901 gggcgagatt gcgaaaaatc atccggaaaa gatgacattt cccaacgctc gtaagccgtt
     3961 tgctgctctt tgggggtggc gaaattagac atattggtca tcaccaccct taccagctaa
     4021 cataatttgc ccttcatcta ccaagcggcg cacaatttgc agaatttgtt tttgctgtgc
     4081 ttccacttca gaaagtttga ccgggccttt ggtttctaaa tcttctttca tcatttcggc
     4141 cgcgcgcgac gacatattgc ggaaaatctt gtcgcgcatt tcttcggtag tgccttttag
     4201 ggcaataatc aatgtttcag actgcacttc tcttaacaat gcttgaatgc ctttatcatc
     4261 cacatccatg atgttatcaa acacaaacat ttcatccatg atttgctgcg ccatgtcttc
     4321 atcgtagctt ttcaggcctt ccatcagctc agcctctttt tcaccgccca taaagttcat
     4381 gatttcggcc gccaccttaa tgccgcccat actttgtttt ttaaggtttt cattgccggt
     4441 gagcaattta gtcatcactt cattcagttc acgcaaagcg atgggcttaa cgccatctaa
     4501 agtggcaatt cttagcatca catcttggcg caaacgatca gtgaaattgc tcacaatttc
     4561 agccgcttgg tcagcttcta aatgcaccaa aatggtagcg ataatttgcg ggtgttcgtt
     4621 tttaattaac tctgccacgc ttggtgcgtc catccacttt aagccttcaa ttccgctggc
     4681 atcactggat tgcaaaatac ggtttaataa attggatgct ttgtcatccc ccagcgcttt
     4741 ggtcaataca gagcgaatat actcatctga atccagccca aaattgctgt tttgttcggc
     4801 ttccagcata aattcactga gtgctgtttc cacttggtca cgcccaaccg attttaatga
     4861 cgccatcgca gcgccaagtt tttgcacttc tttcgggcca agatatttca tcacttctgc
     4921 tgcttcatcc tcacccagcg ccagcattag taccgcgctt ctcattacgc ctgcatcact
     4981 catttatgac tcatttccat tcgtccaatt tgaaaccacg cttgccacaa tgcgtgggtt
     5041 atcctttgcc atttgttttg cggcaagcaa attttgctca tagggcggca ctgcattgcc
     5101 ggattgatta cttaaagtca ccgtggcatc ttcttgacca ccttgtggtg caggtagcaa
     5161 ctgtggtgag gccgtagtga gcttattgag cattggcttt agggcacgtt tatagagcat
     5221 catcaacaca atgatgccca ctaaaaagcg caaggcatct ttgccatatt caatcacggt
     5281 tggatttttc caaagtggca attcagcaac gggttctact ggattttctg caaacgggct
     5341 attcactatc gtcaatgaat cgccacgctc tttgttaaaa cccatggctt gcatagctaa
     5401 atcattgatt tgctgtttct cggccgctga gagcggacga taagtcactt tgcccttagc
     5461 atcaatcact tgcatgttat tgaccaccac cgccacatta atacgtttaa caccgcccat
     5521 tgtttgttgg gtgtaacgca cggttttatc aacttcaaaa ttcgtcgtgg tatttttttg
     5581 gctgttgatt gcggtagctt cagcacctgc cgcagcgcct gccgttgcgc catcggccac
     5641 aatcggtgca gttgcgttag cgggcggctg attagataaa gcaccaggca cgcctgtggc
     5701 agcgccacca ttacctgttt gtgactcgct attttgcatg ctacgaatcg cagcatcttc
     5761 tgtcctctga ttaggcttat acgtttctgc cgcttgttca gtcgatgaaa aatcaatttc
     5821 aacatttgat tcagcccgca catttttagc ccctactatc ggcgcaataa tggattccac
     5881 gcgtttaacg atgctttgct gcatctcgtc aatatattta atttgggttg catctaagcc
     5941 atttgcgcct gctttttttg aagtgtcaga caacaaagta ccgtgctggt ctaccactgt
     6001 cacgttctca atgggcaaat taggcacgct gctggccacc aaatgcacaa cggcggccac
     6061 ttgttgcggg tctaaattgc gccctgctct tacatttaac agtacagatg ccgtcggttt
     6121 aagttgatcg cgcacaaata cggtggcttt tggaattgcc aaatgaatct tagcgccttc
     6181 aaccgcgctg attgattgca cactgcgttc taactcacct tcaagtgcgc gttgaaaatt
     6241 cacttgctct acaaattgtg aaacaccaaa tttttgattt tctaacaatt caaaaccagc
     6301 gttaccgcct ttaggtaagc catcggcggc taatttaagt ctagcttggt gcacttgacc
     6361 tgcaggcacc aaaatcgctg aaccaccttc agaaaattta tacggcacat tcattttttc
     6421 aagcgcggcc acaattgcgc caccgtcttt atctgaaaaa ttggaatata aaacacggta
     6481 atcaggttgc tgactccata accaaaatac caccatcacg gctaccacgg cagccatgcc
     6541 tgctaccagc atcagcttat tgcccaattg cggcatgccg cccataccca tggcggattc
     6601 gtttgttaag gtggcgtttg aggctgccat catcaatctc cagtttctta cgcaataaag
     6661 tcaaaatcag tgagtgtcaa aagaagcgtt ttgtgtttcc tttgacggat taatgactat
     6721 tatgcgcatg ttctgcaaat tcaattttta gaatagagcg gtaatattga gcttatttgc
     6781 accgtttagt attgccactg catgttaaat tgttcaccgt gatacaacct ttagttaaat
     6841 gtgtcttaaa cttaagcaca aaaagtgcta agttttttga attgaatgga gtacaagccc
     6901 aatgaaaaca agtggaatcg acgctggccg aattgaatca atgctggcac aacttaaagc
     6961 tgcggcacag cgcccacagg cagaggcttt aactgcactc aatgtttctg gcacaaaatc
     7021 tgctggcaat gcttcaaaag ttgatttttc aaacgcccta aaagcttcgt tagaccaagt
     7081 taaccaagtg caaaaaagtg ccgagcaatt aggcaaaaat tttgccatgg gcgacgatag
     7141 cgtcagcctg tctgatgtga tgattgccaa ccaaaaagcc aacatttctt ttcaggccac
     7201 cattcaagta agaaataaac tggtttcagc ctatcatgac atcatgaata tgcaggttta
     7261 aaagccttta aaaaggcact caatttacct aacatcaccc cgagaaaaat tttatgccct
     7321 gcgagccttt atttataagg ctcgtagagc atcgaaaaag gttggtgtaa acatcagcgc
     7381 caattttcga ttttttcctc aaacttttct gcttgttgcg attgcgcttt ttctgcttcc
     7441 agccgataaa tccaagccaa aatttcagca atcgccaaat acaattctgg tggaatatgc
     7501 gcatctaaat ccacctgcat gagcaaattc accaaatctt tggattcatg cacaaacacc
     7561 ttgtgttctt ttgccaaggc aataatctgt tcagctactg ccccgtgccc ttttgccacc
     7621 acttttggcg cgtaatcatt ggcgccatag gcgagcgcaa tggcttgctg atgatggtgc
     7681 ggttgcgcga gattattcat attggcctac cgtcagcgcc tcaattgctt ggcctttatt
     7741 caccattgat gcaaaaagtt gctggcgttc ttttttaaag gttgcgagcg tttgcgggtt
     7801 ttcagtcaac atatgaatgc ttatgcggcc atctaccacg ccgatgcgcg ccgacacttt
     7861 gcctaaattt ggcaaatgca tggtgatttc actactgacg ggtttggttt tttgggtttc
     7921 gtcttggctg gattgttctg catgttcgcg cggctcatca aaggtcacat cccaatccat
     7981 ggtttgattt tgccaaattt cgccatgcca cgacaaacgt tgatgctcta acgcggccaa
     8041 ttgttgtgcc ggtaaagtat tgagcacggt gttgggcgta ttgttcgcca agttttgcaa
     8101 ctctggtcgc atgctctcga ttgtgcgttt acctgccacc acttcagcca gatgcgattc
     8161 cagaaataat ccgctacgtt gtagcgcatt ttttaaatcc aggcccagtt gctgtgggtt
     8221 atacggctgc tgcgtcacca cgccattggc ctgatagcgc atagaagtgc cttcttcatt
     8281 ggcctgcttg ataatgctgc ccagaagaaa acccgtttgg ctgatagccg cagtggcagc
     8341 actattaaat ttatcggctg ataataaata aaaagttggc acaggttgcg attgcataaa
     8401 ccgcagtgaa agcgtttggc ccttgtcaac ttggctgcca agatccattt ttaacaattg
     8461 gccttcaatt tcaaccagtt gttttccatc ggtggtttta gccgccaccg tggctaaata
     8521 ttcttgccca accaaaaatt gggtagcgcg gctcaaaagc gcctgagctg ggtcaccaat
     8581 tttctccacc gcaacaatcg gcaatacctt gggtacaggt aaaatgcttg tcaggtcggc
     8641 tggtttaaac atttaggtca cgctcgctgc catcgccggc taatgctggt aagcctgcga
     8701 tagcttttgt tccatgtggt tgctatgcat catcgcattg agtttagcca tccatggcga
     8761 gacaatatta cgaatttcac ggtcattggc gagaatgctt tttacgctgc tgagtttgcg
     8821 cgccagttct tcttcactaa gcttgctgga tgattccatg accttaatca tttgcaccat
     8881 ctgcgcgcaa tggctttcca tttcagccaa agcatcccat tcttgctgct ttgccgcatc
     8941 taacatgcgg tgcatgatgc ctgccacaga ttcataaagt acgacagtat tttgctgttc
     9001 cattctcacg atgctcgctt aagttaaatt gattgagaat aagc
//
LOCUS       DAOQSI010000080         8955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_7240_length_8955_cov_4.443258, whole genome shotgun sequence.
ACCESSION   DAOQSI010000080 DAOQSI010000000
VERSION     DAOQSI010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 8955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8955
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7240_length_8955_cov_4.443258"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PKL42_06340"
     CDS             complement(<1..626)
                     /locus_tag="PKL42_06340"
                     /inference="COORDINATES: protein motif:HMM:NF014324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HNU66733.1"
                     /translation="MNRTPLWVSVLLFIFSTTAQAQLVGTNVEAAVAYCKTIFSETTQ
                     ADERLSCFDNIQLTADVEQASAAKKADTQDCKIASDKPCRYLDRKWRLSSSESKDLTD
                     LETHGLNYLVVTKTSNPNHIASSPRHLDGIDRDLDNQDLKFQVSFKTELMRSIPFVRD
                     LPYVDTSRIWAAYTQQSYWQVFDSKNSRPMREHNFSPEIILSLGLKAP"
     gene            complement(684..1742)
                     /locus_tag="PKL42_06345"
     CDS             complement(684..1742)
                     /locus_tag="PKL42_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNU66734.1"
                     /translation="MEYTKLGQSALNVSKVCLGTMTFGDQNTQAEAHAQLDYAISQGI
                     NFIDTAEMYPVPPKAETFTRTETIIGHWLKHQVRDKIILGSKVAGRNRGLNWIRGGDD
                     SLTRANIRNALHDSLKRLQTDYIDLYQLHWPERNVPLFGQYQFDPKLELDAQGNQKDW
                     VSIHTQLETLAELIQEGKVRAIGLSNEQPWGVMEFLRISKEYNLPRIATLQNSYHLMN
                     RSIDFGLSEVLYRENVSLLAYSPLAFGHLTGKYVDNPQTQGRVTMFLGYAQRYTKQNV
                     SPASAAYAKLARAHGLTPTQLALSFVYHRWCVASTIIGATSMLQLKENIGAWQIKLSA
                     EVLQEIEHLHLMMTNPAP"
     gene            1931..2467
                     /locus_tag="PKL42_06350"
     CDS             1931..2467
                     /locus_tag="PKL42_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNU66735.1"
                     /translation="MPLKVRLYINRMHQLDNSVCIAVNHTSQYRLIRNYFRLISRLGD
                     GLFWYILMLGIFMVKGVDGITAVTHMAFAGLTGTLVYKWLKGKTLRPRPYEVHQDIWL
                     TGTPLDKFSFPSGHTLHAVVFSLVAATYYPQLAFILVPFTTMIGLSRVVLGLHYPSDV
                     IAGALLGAVIVKLSFLIV"
     gene            complement(2464..3312)
                     /locus_tag="PKL42_06355"
     CDS             complement(2464..3312)
                     /locus_tag="PKL42_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HNU66736.1"
                     /translation="MMNAFWWLPIFLYSADANAWGLVTHVYFAQSLLWAMPLLDPRLQ
                     RAIRNFPELVMAGACLPDLAIVSRRYQHTHLWESGHRLMSAAQTEEETALAIGYISHL
                     YIDVIAHNHFVPAHEAMWHKNEIFTHVVSEWAMDGHLASLVNILPGQLLNRHHAIITR
                     FISIQFRCSVNVTSRALKRLAFWDGILRTVKLPNLIHRLVSILDKRVFKHFVYYLAKT
                     QVAVADIGEVLNGGNPILEPELKNLSVEQLDIWREQCVVHLGLLHPTPVQYFNKTLIN
                     PNYPDS"
     gene            3475..4707
                     /locus_tag="PKL42_06360"
     CDS             3475..4707
                     /locus_tag="PKL42_06360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNU66737.1"
                     /translation="MKILFLSDVYFPRINGVSTSIRTFVEQMQNLGHEVHLIAPDYEI
                     LTDDEAWIKRVPGRNIYFDQEDKLMKFGHVMQLLPELQQEKYDIIHVHTPFVAHYAGL
                     KLAAKLNIPCIETYHTFFEDYLHHYLPWLPKRMAKGIARLISKRQCNSVDAIVAPSKP
                     MLDVLRQYGIYTPSEVIPTGLQDSSFKTGNGQAFRSLYGISLERPMLLYVGRVAFEKN
                     IDFLLEMTKKLKEKQPDILLLVTGEGPAEASLHKLTQSLSLEDNVKFIGYLDRNTELN
                     ACYASADAFVFASKSETQGLVLLEAMAQGTPVVAIAELGTASILVEGKGALIAPDNTL
                     QFSERVHELLVNPLHRFELGKQAKTYALEKWTAKLQAERMIQFYEEIIKSKSSCQLNA
                     NNQLANMDRETSHLNASF"
     gene            5026..5313
                     /gene="groES"
                     /locus_tag="PKL42_06365"
     CDS             5026..5313
                     /gene="groES"
                     /locus_tag="PKL42_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNU66738.1"
                     /translation="MKIRPLHDRVIVKRSEEERTTASGIVIPDSATEKPDQGVVQAVG
                     NGKRDENGKVIALDVKVGDKVLFGKYAGQTVKVDGEELLVMREEDIMAIVE"
     gene            5358..7001
                     /gene="groL"
                     /locus_tag="PKL42_06370"
     CDS             5358..7001
                     /gene="groL"
                     /locus_tag="PKL42_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147112.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNU66739.1"
                     /translation="MAAKDVRFGDDVRQKMVAGVNVLANAVKVTLGPKGRNVVLERSF
                     GAPTITKDGVSVAKEIELKDKFENMGAQMVKEVASKTSDIAGDGTTTATVLAQAIIRE
                     GMKSVAAGMNPMDLKRGIDKAVAAAIADLKTQSKPCTTSKEIAQVGSISANSDETIGK
                     IIADAMDKVGKEGVITVEDGSGLESELDVVEGMQFDRGYLSPYFINNQERQIALLDNP
                     FVLLHDKKISNIRDLLPTLEQVAKSGRPLLIIAEDVDGEALATLVVNNIRGILKTTAV
                     KAPGFGDRRKAMLEDIAMLTGGTVIAEEVGLKLENVTLENLGQAKRIEVGKENTIIID
                     GAGNETNIKSRIAQIKTQIEEATSDYDREKLQERVAKLAGGVAVIKVGATTEIEMKEK
                     KARVEDALHATRAAVEEGIVAGGGVALIRARAAIAKVKGDNHDQDAGVKIVLRAVEEP
                     LRQIVQNAGVEPSVVVNNVEAGKGNYGYNAANETYGDMIEMGILDPTKVTRSALQNAA
                     SVAGLMLTTDCMVAEHPKEDGPAMGGGDMGGMGGMGGMM"
     gene            7171..7866
                     /gene="ung"
                     /locus_tag="PKL42_06375"
     CDS             7171..7866
                     /gene="ung"
                     /locus_tag="PKL42_06375"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNU66740.1"
                     /translation="MPLEKPGQLHPSWQAVIGDELEKPYMQNLRAFLQGEKSAGKTIY
                     PPSPLIFNAFNHTPFDKVRVVIIGQDPYHGQDNGQPQAHGLSFSVPAGVGLPPSLQNI
                     FKEISSDLGIRMSGKGDLTPWADQGVLLLNATLTVEQAKAGSHQTRGWEAFTDAAIAA
                     LNAHRQNLVFVLWGNYAQKKGAIIDEKKHLVLKSVHPSPLSAHRGFFGNHQFSKINQY
                     LIAHQQPAINWQL"
     gene            7991..8800
                     /locus_tag="PKL42_06380"
     CDS             7991..8800
                     /locus_tag="PKL42_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HNU66741.1"
                     /translation="MKEYMYNQNSFVIALVLFTLTAVAIERSYRYGIKSKHKNNSTYK
                     SHVDAVLTSLLSLLALLLAFTFSIALQRYDSRSDAVVEEANAIGTTYLRSALLPSEIR
                     NEAARIIGEYLDVRVKASSLTLDQISERGVLLNQATQLQTVLWNQAIKAAEIEPNPVT
                     SGLYIQALNELIDAYGRRIASIDRHVPESVLFLLSIVFIVVGGISGYAQGIASQRPTF
                     INYLSVGLFVLIVFLVIDLDHPRRGLIKVSQQSLLDLAISIDKQLPQVTQK"
BASE COUNT         2691 a         1891 c         1873 g         2500 t
ORIGIN      
        1 tcaggcgcct ttaagcctaa ggataaaatt atctccgggg aaaaattatg ctcacgcatc
       61 ggccgtgaat ttttgctatc aaacacctgc caataagatt gttgcgtgta ggccgcccaa
      121 attctagagg tatccacata gggtaagtcg cgtacaaaag gaatgctgcg catcaactct
      181 gttttaaaac tcacctgaaa ctttaaatct tggttgtcta aatcacgatc aattccatca
      241 aggtgtcttg gcgaactggc aatatggtta gggttggatg ttttagtgac cacaagataa
      301 ttcagcccgt gcgtttcaag gtcggttaaa tctttacttt cactactact taaacgccat
      361 tttctatcga gatagcgaca tggcttatca gatgcgattt tgcaatcctg tgtatcggcc
      421 ttcttagccg cactggcttg ctctacgtca gctgttaact gaatattatc aaagcatgat
      481 agacgctcat ctgcttgcgt ggtctctgaa aagattgttt tacaataggc aaccgccgct
      541 tcaacattcg tgcccaccaa ttgcgcttgt gcagttgttg aaaaaataaa taaaagcaca
      601 cttacccata acggggttcg gttcattgtt tgttccaatg taaaaatttt acgattgtaa
      661 agtttgaagg tgctgatagc gccctaaggt gcggggttag tcatcatcag gtgtaaatgc
      721 tctatttctt gaagtacttc agcagacagc ttaatttgcc acgcaccgat attctcctta
      781 agctgtaaca tactggttgc gccaataatg gtactggcaa cacaccagcg atggtaaaca
      841 aaacttaagg caagctgggt tggcgttaag ccatgcgctc gcgctagttt agcgtaagca
      901 gcactggctg gggaaacatt ctgtttagtg tagcgttgcg catagcctag aaacatcgtc
      961 acccgacctt gggtttgcgg gttatcaaca tacttgcccg ttaaatggcc aaatgcaagc
     1021 ggagaatagg ctagcaagct gacattttca cgatacaaaa cttcactcaa gccaaaatca
     1081 atactacggt tcattaaatg atagctgttt tgtaaagtag caatgcgcgg cagattgtat
     1141 tctttgctga tacgcaaaaa ctccatcaca ccccatggct gctcgttact tagcccgatt
     1201 gcgcgcactt ttccctcttg aatcagttcg gctaacgttt ctaactgcgt atgaatactt
     1261 acccaatcct tttggttgcc ctgtgcatct aattctagct ttggatcaaa ttgatattgc
     1321 ccaaataatg gcacattgcg ctcaggccag tgcaattggt ataaatcgat gtagtccgtt
     1381 tgcagacgct ttaatgaatc gtgcagggcg ttgcggatat tcgcgcgtgt caatgaatca
     1441 tcaccaccgc gaatccaatt taaaccacgg tttcgcccgg ccactttact gcctaaaata
     1501 attttgtcgc gcacttgatg ttttaaccaa tgcccgatga tggtttcagt gcgggtaaac
     1561 gtttcagctt tgggcggcac aggatacatt tctgctgtat caataaaatt aatcccctga
     1621 gaaatcgcat aatcaagctg cgcatgcgct tctgcttggg tattttggtc gccaaaggtc
     1681 atcgtaccca aacaaacttt ggagacattt aatgcggatt gaccgagttt tgtatattcc
     1741 atttgcaacc ctttattcat tgcaacttca catgaaatta gttctatttg aatgatcaat
     1801 gagttactta caacccacta taaatcactt tcagcttcaa gataacttca ttttttattc
     1861 atcaattgtt catatttcaa tgagattcta accttcaaac taaaaaattg aaaaattaag
     1921 gaggtttaca atgccactta aagtacgttt atacatcaac cgcatgcatc aactggataa
     1981 ctcagtgtgc attgctgtta accatacaag tcagtatcgc ctaatacgaa attacttcag
     2041 actgattagc cgattaggtg atgggctttt ttggtatatt ctgatgctgg gtatttttat
     2101 ggtcaaaggt gtcgatggta tcactgcagt gacacacatg gcatttgcag gcttaactgg
     2161 caccctcgtt tataaatggt taaaaggcaa aaccctgcga cctcgccctt acgaagttca
     2221 tcaagatatt tggctaacag gaacgcctct tgataaattt agctttcctt ctggccacac
     2281 ccttcacgcc gttgtattta gcctggtggc ggcaacctac tacccgcaac ttgcatttat
     2341 tcttgtacct ttcacgacaa tgattggtct ctcccgggtt gtacttggtt tacattatcc
     2401 gagtgatgtg attgctggcg ccttgttggg tgctgtgatt gtcaaactgt cttttcttat
     2461 agtctagcta tctggatagt taggatttat caaagtctta ttaaagtact gcacaggggt
     2521 tggatgcaat aatcctagat gcactacgca ctgctcacgc caaatatcca attgttccac
     2581 actgaggttt ttaagttctg gctctaatat tggatttcca ccatttaaca cttcaccaat
     2641 atcagctacc gcgacctgtg ttttagctaa atagtagaca aaatgcttga atactcgctt
     2701 gtctagaatg cttaccaagc gatgaattaa attgggtaac ttcacagtac gcaatatacc
     2761 atcccaaaac gctaggcgtt ttaatgcgcg gcttgtgaca ttgacagaac accgaaactg
     2821 aattgaaata aatctagtga tgatcgcgtg gtgcctgttt agcaactgcc ctggcaagat
     2881 atttactaag gaagctaaat gcccgtccat ggcccactct gatacaacgt gcgtaaatat
     2941 ttcattttta tgccacattg cttcgtgcgc gggaacaaaa tgattatgcg cgattacatc
     3001 aatatataaa tgactgatat agcctattgc aagcgctgtt tcttcttcag tttgcgctgc
     3061 agacatcaat cgatgcccgc tttcccataa atgcgtgtgt tgatagcgac gactcacaat
     3121 cgcaagatct ggcaaacacg cgcccgccat gaccaattct ggaaaatttc taattgctct
     3181 ttgtaggcgt ggatctagca acggcatcgc ccaaagcagt gattgtgcaa aataaacgtg
     3241 tgtcaccaat ccccatgcat tagcatctgc tgaatacaga aatattggta accaccaaaa
     3301 cgcattcatc ataaacgttg gcaagcgcat gggctataaa ttgtcatgca atcattaaaa
     3361 attaattaca aaaatatgat tttttggtga caggctttca atcaacattt cattaaaacg
     3421 tcatattgag acgttattgt ttgcctaaat tcattacaca tggccttaat ctatatgaaa
     3481 atattatttc tttccgatgt ttattttcca cgcattaatg gtgtttctac ctccattagg
     3541 acatttgttg aacaaatgca gaaccttggt catgaggtgc atcttattgc gcctgattac
     3601 gaaatattga cagacgatga ggcctggata aaacgtgttc caggacgaaa tatttatttt
     3661 gatcaagaag ataaactcat gaagtttggt catgtcatgc agctattgcc agagctgcag
     3721 caagaaaaat acgatattat tcatgtgcat acaccgtttg tcgcgcatta tgctgggtta
     3781 aaacttgctg ctaagttaaa cattccttgt atcgaaactt accatacatt ttttgaagat
     3841 tacttacatc actacttgcc atggctacct aaacgtatgg caaaaggcat tgcaagactc
     3901 atttctaaac gtcagtgtaa ttcagttgat gccatagtag cgccttcaaa accaatgtta
     3961 gatgtgctac gccagtacgg catctacacg ccatctgaag tgattcctac tggtttacaa
     4021 gatagcagct ttaagaccgg caatggtcaa gcctttcgct cactctatgg catctcatta
     4081 gagcgcccaa tgttacttta tgtaggcagg gttgcatttg agaaaaatat cgacttccta
     4141 cttgaaatga cgaaaaaact taaagaaaaa caacctgata ttttactcct tgtgactggt
     4201 gaaggccccg cagaagcttc cttacataag ctcactcaat cactatcctt agaagataac
     4261 gtcaaattca ttggttactt agaccgcaac accgagttaa atgcttgtta tgcgtcagcg
     4321 gacgcttttg tgtttgcctc taaaagtgaa acacaaggct tagttttatt agaggccatg
     4381 gcgcaaggaa cacctgttgt agcgattgct gaattaggca cagcctcaat tttagtggaa
     4441 ggaaaaggcg cattgattgc tcccgacaat acacttcagt tctccgaaag ggtgcatgag
     4501 ttactggtaa accctttgca tcgatttgaa cttggtaaac aggcaaaaac ttacgcacta
     4561 gaaaaatgga cagcaaagct acaagctgag cgaatgattc aattttatga ggaaatcatc
     4621 aaaagcaaat catcttgtca acttaatgca aataatcagt tagccaacat ggacagggag
     4681 acaagtcatt tgaatgcaag tttttaatag cgtaatccat aaaaagcaaa cgatttatca
     4741 agcaccaacc cgctgcatgc gattgcaatg aagcgttgtc accaagattt tccatgaaaa
     4801 tatcatcttc caaacaaaat aactcagctc tattttagat ttcaaaaatc taaaaatgaa
     4861 tgaaaaataa tatcaaaata ttagcgctta cctcttgaaa taagttgaat atgccccaaa
     4921 ttagcaatca aggcatgcga gtgctaaaat accgcattgc ccttgcaatt cataaacgca
     4981 atgacattca atagatttat agcataactt atcaggagat actgtatgaa aattcgtccc
     5041 ttacacgacc gtgtgattgt taaacgttct gaagaagagc gtacaaccgc ctctggcatt
     5101 gtgattccag attcagcgac tgaaaaacca gaccaaggag tggtgcaagc tgttggtaat
     5161 ggcaaacgtg atgaaaatgg caaagtgatt gccctagacg ttaaagtggg cgacaaagtg
     5221 ctatttggta aatacgcagg ccaaaccgtt aaggtggacg gcgaagaatt attagtgatg
     5281 cgcgaagaag acatcatggc aattgtcgag taatttctaa ttaagaatac attgaagaat
     5341 atctaggaga attgaatatg gcagcaaaag acgtaagatt tggtgatgac gttcgtcaaa
     5401 aaatggtagc tggcgtgaat gtactcgcca acgcagtgaa agtcacttta ggcccaaaag
     5461 gccgtaacgt tgtattagag cgctcatttg gcgcaccaac cattactaaa gatggtgttt
     5521 cagtagctaa agaaatcgaa ttaaaagaca aattcgagaa catgggcgca caaatggtga
     5581 aagaggttgc gagcaaaact tctgatatcg ctggcgacgg cacaacaaca gcaaccgtgc
     5641 tggcacaagc aattattcgt gaaggcatga aatcagtcgc tgccggcatg aacccaatgg
     5701 atttgaagcg cggcattgat aaagcggttg ctgcggccat tgctgattta aaaacacaat
     5761 caaaaccatg tacaaccagc aaagaaatcg cgcaagtagg ctctatctct gcaaactctg
     5821 atgagacaat tggtaaaatc attgctgatg cgatggacaa agtaggtaaa gaaggcgtga
     5881 ttacggttga agatggttct ggccttgaaa gcgaattaga tgtagttgaa ggcatgcagt
     5941 ttgaccgcgg ctacttgtca ccctacttca tcaacaacca ggagcgtcaa attgcgttgt
     6001 tagataaccc attcgtattg ttacacgaca aaaaaatctc aaacatccgt gatttattac
     6061 ctactttaga gcaagtcgct aaatcaggtc gtccattatt gattattgca gaagacgttg
     6121 atggtgaagc gttggcaacc ttggttgtaa acaacatccg tggcatcttg aaaacgactg
     6181 ctgttaaagc ccctggtttt ggtgaccgtc gtaaagcgat gttagaagac attgctatgt
     6241 tgactggcgg taccgtgatt gctgaagaag ttggtttgaa acttgaaaac gtaacgcttg
     6301 agaatttagg tcaagctaaa cgtattgaag taggcaaaga aaacactatt attattgatg
     6361 gtgcaggcaa tgaaactaac atcaaatcac gtatcgcaca gattaaaacc caaatcgaag
     6421 aagcaacaag cgattacgac cgtgaaaaac tacaagaacg cgtagcgaaa cttgctggcg
     6481 gtgttgcagt aattaaagta ggtgcaacca ctgagattga aatgaaagag aaaaaagcgc
     6541 gcgtagaaga tgcattacac gcaacacggg cagcggttga agaaggtatt gtagctggcg
     6601 gtggtgtagc actcattcgc gcacgtgcgg caattgcaaa agttaaaggt gataaccacg
     6661 accaagacgc aggtgttaag attgtattgc gcgcagttga agaaccatta cgtcagattg
     6721 tgcaaaacgc aggtgttgag ccttcagtag tcgtgaataa tgtagaagct ggtaaaggca
     6781 actacggtta caacgctgct aacgaaactt acggcgatat gattgaaatg ggtattcttg
     6841 acccaactaa agtgacacgc tctgcattgc aaaatgcagc ttcagtggcc ggcttaatgc
     6901 tgacaactga ctgcatggtc gctgagcatc caaaagaaga cggtccagca atgggcggtg
     6961 gtgatatggg tgggatgggc ggcatgggcg gcatgatgta gacttttgat gttgcatcaa
     7021 cttgtccaac ttaggtcggg ttgatgcaaa cactaaactc aatcttgcac ctccgtagta
     7081 ttaaaacctc gcttcggcga ggttttttat tgcctatcaa cttataatcc cttttataat
     7141 cccttaactt tcataaaaca cgcttagaca atgccattag aaaaaccagg tcagttgcac
     7201 ccttcatggc aagccgtgat tggtgatgag cttgaaaagc cctatatgca gaatttacgc
     7261 gcttttctac aaggtgaaaa atctgcgggt aaaaccatat acccacctag tccgctaatt
     7321 tttaatgctt ttaaccacac tccttttgac aaagtacgcg tggtgattat tggtcaagac
     7381 ccttatcatg gccaagataa tgggcagcct caggcacatg gcctgagttt ttcagtgcct
     7441 gcaggggtag gtttaccgcc atcattacaa aatattttta aagaaatttc atctgattta
     7501 ggcatacgca tgagtggaaa aggcgattta acaccttggg ccgaccaagg tgtattgctg
     7561 ctcaatgcca cactgaccgt tgaacaagca aaagcgggct ctcaccaaac gcgcggctgg
     7621 gaagccttta ccgatgcggc aatcgctgct ttaaatgccc atcggcagaa tctggtgttt
     7681 gtgctgtggg gcaattatgc gcaaaaaaaa ggcgcaataa tcgatgaaaa aaagcatttg
     7741 gtacttaaat ctgtacaccc atcgccttta tctgcccatc gcggtttttt tggtaatcac
     7801 cagttttcaa aaatcaatca atacttaatt gcacaccagc aacctgccat caactggcaa
     7861 ctttaaatag ttaaatatgc caagagaaaa ttgttgccct actaatgaat caattgtagt
     7921 attttttcct gcatacaata actaagcaat ccatgcctat tgctgtgctt ttcacaacac
     7981 agaaagcgcc atgaaagaat atatgtacaa tcaaaatagt tttgtcatcg cactggtgct
     8041 ttttacttta acggcagtcg caatcgaaag aagttatcga tatggcatta aatcaaaaca
     8101 caaaaacaat tcaacttata aatcccatgt tgatgcagta ttaacatcgc tgctcagtct
     8161 tttagccctt cttctcgcct ttactttttc aattgcgttg cagcgatatg acagccgcag
     8221 cgatgctgta gtggaagaag ccaatgccat tggtacaact taccttcgct ctgccttgtt
     8281 gccttctgaa atacgtaatg aggctgctcg cattataggc gaatatcttg atgtaagagt
     8341 aaaggcgagc tcgctgacgc tcgatcaaat cagcgagaga ggcgtgttgc ttaatcaagc
     8401 cacgcaatta caaactgtgt tatggaatca ggcaatcaag gctgcagaaa tagagcctaa
     8461 ccctgtgaca agcggtctgt atattcaggc actgaacgaa ctgattgatg catatggcag
     8521 acgcattgct agtatagaca gacacgtacc tgaatcagta ttgtttttgc tgtcaattgt
     8581 tttcatcgtt gtcggaggca tttctggata cgcacaaggt atcgccagtc agcgaccgac
     8641 tttcattaac tacctctcgg tcggcttatt tgtattgatt gtctttttag tgattgacct
     8701 tgatcaccca aggcgcggac tcatcaaagt cagccaacaa agcctgttgg atttagcaat
     8761 ttcaatagac aaacaactcc cgcaagtaac tcaaaaatag ccaagctaaa catagtggaa
     8821 atagcaatct aatccccagt gccaatcact taaaaccctg cgagaaaaaa ataatgctct
     8881 gcaagccttt atttataagg cttgcagagc atgcaaaaag gttgtgggtg aaataagcca
     8941 aaaatgtgct ttttt
//
LOCUS       DAOQSI010000081         8749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_7492_length_8749_cov_5.459167, whole genome shotgun sequence.
ACCESSION   DAOQSI010000081 DAOQSI010000000
VERSION     DAOQSI010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 8749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8749
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7492_length_8749_cov_5.459167"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="PKL42_06385"
     CDS             complement(<1..910)
                     /locus_tag="PKL42_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66742.1"
                     /translation="MLFNIGLAMCVAHASESSENAGFFILSDRSYGSQEIAQIRLEVQ
                     DVQAVHQNAGIDIAVYKVPNPIAFLQKQSNLHRINTQAQPAQPGVWNAIVAVWDYVAR
                     SARQLARKLFSEEARQTTVEAAPDLHIEKIQHPATRHSAYKPLKGYTLTARFRYPLQY
                     GKDVKSADALLSGANVEGNINFDDAAPAEQNAQAVAEPAQQPAFQTLSNGNVYIPIGQ
                     LSPGLYIVEAMLDEQRAVTMVFVGDTVAVTKTSSNGLFVWTVDRLSGAPVANVASHWS
                     DGVGELAKGVTNQQGWVQLNHAAPEQT"
     gene            complement(946..1710)
                     /locus_tag="PKL42_06390"
     CDS             complement(946..1710)
                     /locus_tag="PKL42_06390"
                     /inference="COORDINATES: protein motif:HMM:NF018387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HNU66743.1"
                     /translation="MQQQTVKSDLMIAKHQPSHGRRGVLGGFAALLLVLASPKQVFAL
                     MSKDERPSLGYPSNQRNSNQVRLNTSQMQAFRSWLQLIVHQQLNLGPTPRWQQRDCAG
                     LVRFASAEALRQHDATWLKANGFLGKKIPPELNLTVEQRAALRHAWLRADGSQGAFVS
                     ALELVQNNTQPIGRDMAHAEVADLLLFDQGQEQHLMIWMGRYVAYHTGKVAPNDNGLR
                     AYPLAKLMSWKDNRWQPQAGNPNFIGIFRLFFLSTS"
     gene            complement(1717..3681)
                     /locus_tag="PKL42_06395"
     CDS             complement(1717..3681)
                     /locus_tag="PKL42_06395"
                     /inference="COORDINATES: protein motif:HMM:NF021432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 family protein"
                     /protein_id="HNU66744.1"
                     /translation="MQSNNNNSNASAPPSSSLFEKVKQSFSHRKKTWLIAGFLLAGAI
                     LAGIFFKIPLAKNSQNGVALAQPDVWVHSQNLSLLPRDLLQVPLLKTLLTEDFVYFYA
                     EDEDWLSLQGAMRRISFEHALNWSDTLLKNIAEAPADIYMWHDDSHALRYWALSLERD
                     QLSAVAQKLATLKLTADKQLQQIGSVRIAGTSVPVLQLSLSSRRQMVLAAHQNRLVLL
                     SDIDMVSHADNELDDQTAKLISRLLSEDSKVRAEVIAEWQVTANAQPVHESKQTILVS
                     NRLFAQGYAPFVPYLGALRFDFDGKQWQTQANIADNTFNPNIWRHLPANAAFCVATPI
                     DWAQVQTALAGAKNLNTQLNLPAEFASSGAICWYAEKSDDIAQPLFVALRQPSQKKQE
                     MLTALFDWGVATNQDHLKALIALNRQKRHLKNQMEYAASNLEAAKQEKIDGKLDREQK
                     AARQAQKEAAIESSKAEVKVAETALAELAPAITAVKQAAEAPALIAKENAIVQQGAFT
                     VVSRKLSIEATSEKNPVLAFDNEVVYFSTNPALTQRAVSVGEKKYPNLQESAVVLQQS
                     TQQFLYVNPKKLSTLLTATGHAALPQETKSNLRAAFDYHMPARFAALSQQRPFSLVLE
                     SKASKTKQAGSSAHDIAQWQPLVWRTAPER"
     gene            complement(3786..5075)
                     /gene="gabT"
                     /locus_tag="PKL42_06400"
     CDS             complement(3786..5075)
                     /gene="gabT"
                     /locus_tag="PKL42_06400"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HNU66745.1"
                     /translation="MTTQPPSNQDLHQRRLAATPRGIGVMANFFIERALNSEVWDVEG
                     RRYIDFAGGIAVLNTGHRHPKMVAAVEAQLQQFTHTCYQVLPYENYVTLAERINQLTA
                     GSHAKKTCFFSSGAEAVENAIKIARAATNRSGVIAFSGAFHGRTMMGCALTGKVVPYK
                     VGFGPFPAEIYHVPFPTELHGVSVEDALHAIQTLFKADVDPMRVAAIIIEPIQGEGGF
                     YVTPPALMNALRTLCDEHGILLIFDEVQTGFGRTGKLFAAEHFNVTPDIMVMAKSLAG
                     GMTLSAVCGKAEIMDGPAPGGLGGTYAGNPLAIAAAHAVIDIIEEEQLVARANVLGAK
                     LTSRLKAMQVNTPALKEVRGLGSMIAAEFFDPVTQQPSPEMTKRVQQAALAEGLVLLT
                     CGVYMNAVRFLYPLTIQDAVFEEALSIIERAISTIRE"
     gene            complement(5245..6738)
                     /locus_tag="PKL42_06405"
     CDS             complement(5245..6738)
                     /locus_tag="PKL42_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HNU66746.1"
                     /translation="MADTTTDWHARAKTLKIDGRAFIGGVRMPAAGGQTFECFSPIDN
                     RKLADVASCQEIDVNLAVACARTAFNDRRWAGLSPRERKQAMIRFADLLITNREELAL
                     LETLDMGKPISASLSVDVNSAANSLRWFGEAIDKVYDEIAPTADNTLGMITREPMGVV
                     GAIVPWNYPILMACWKIAPALASGNSVVLKPSEKSPLTALRLGDLALEAGIPAGVLNI
                     IPGFGEQAGTPLAMHMDVDCIAFTGSTGVGKKIQIMAGQSNLKRAWCELGGKSPNIVF
                     ADCKDLDKAAAASIGSIFYNQGESCNAPSRLLVERAIKDAFVKKLLAHLPDYQPGDPL
                     DEKTTMGAIVDCGQMENVLKYIASGKAQGAKLLAGGAQVRTETGGFYISPTVFDAVTN
                     DMTIAAEEIFGPVLSIIAFDSEEEAIKIANDTPYGLAAAIWTQDISRAHLVAQRLRAG
                     TVHVNSYDEDDITVPFGGYKQSGNGRDKSLHALEKYTELKTTWIRLK"
     gene            complement(6744..8204)
                     /locus_tag="PKL42_06410"
     CDS             complement(6744..8204)
                     /locus_tag="PKL42_06410"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HNU66747.1"
                     /translation="MTLQIKHKSLIKNNQTLLAGNWVGADSGETFTVTNPANGQAIAN
                     VPRMGKAETERAIIASQTAQKQWKMLTAKARAAILKRWFDLMVEHHEDLAQILTAEQG
                     KPLAEARGEITYGASFVEWFAEEAKRVYGEVIPAPTADRKLLVIKQPIGVTAAITPWN
                     FPVAMITRKAAPALAAGCSMIIKPAENTPLSAIALAVLAEEAGVPAGVLQVLTGKARE
                     IGAVLCESPIVTKLSFTGSTEVGRILMRQCADTIKKLSLELGGNAPFIVFDDADLDAA
                     VEGAMVSKFRNAGQTCVCANRLFVQDGVFDAFAAKLAAKVATLKVGDGTAEGVTQGPL
                     IDNAAVEKVESHVADAVAKGATLLQGGKRHANGGTFYEPTVLADVSPNALIFKEETFG
                     PVAPLFRFKTDEDVIAMANNTEFGLASYFYSRDIGRIWRVAEALEYGMVGVNTGMISN
                     EVAPFGGVKQSGLGREGSHHGIDEYLEIKYIAMAGL"
     gene            complement(8254..>8749)
                     /locus_tag="PKL42_06415"
     CDS             complement(8254..>8749)
                     /locus_tag="PKL42_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNU66748.1"
                     /translation="SLLLMIISVQHWLGFPEKGLFTILLGHALLGTAYAAVVVTSRLR
                     EMDSKLDEAAMDLGCKPWQVFYLVTLPLLLPALISAFLLTFTLSFDDVVLSSFLSGPG
                     YSTMPMVIFSRARLGLNPTINAVATVTIVVVTFAVIASSFYTSHQARKRKREEAQAYS
                     DSNK"
BASE COUNT         2264 a         2210 c         1906 g         2369 t
ORIGIN      
        1 aggtttgctc aggggctgca tggtttaact gcacccaacc ttgctgattg gtgacgcctt
       61 ttgctaattc acctacgcca tcactccaat ggcttgcaac attggcaaca ggtgcgcctg
      121 acaaacggtc aaccgtccag acaaacaatc cattcgagga ggttttagtg acagcgacgg
      181 tatcgccaac aaacaccatc gtaactgcgc gttgctcgtc gagcatggct tctacgatgt
      241 acaaaccagg tgataattga ccaatcggaa tatatacatt gccattgctc aatgtttgaa
      301 atgctggctg ctgtgcaggc tcagcaactg cttgagcgtt ttgctcggct ggcgccgcat
      361 catcaaaatt aatgttgccc tcaacgtttg cgccacttaa taatgcatct gcagatttaa
      421 catctttacc atattgcaaa ggatatcgaa atcgagcggt caacgtataa cccttaagcg
      481 gcttgtatgc tgaatgacgc gttgctgggt gctgaatctt ttcaatatgt agatcaggtg
      541 cggcttctac ggtcgtttga cgcgcttctt ctgaaaacaa ctttctggca agttgtcgcg
      601 cgctacgtgc cacatagtcc caaacagcaa ctattgcatt ccaaacacct ggctgggcgg
      661 gctgcgcctg cgtgttaata cggtgcaaat tgctttgttt ttgcaaaaat gcaatgggat
      721 ttggtacttt atatacagca atatcaatac ccgcattttg atgtaccgcc tgcacatctt
      781 gcacttctaa tcgaatttgt gcgatctctt ggctgccata acttctatct gaaagtataa
      841 aaaaacctgc attttcactg ctctcacttg catgtgcgac acacatagcg agcccgatgt
      901 tgaacagcat aaatgctgat gtcattattt ttattaaatt aaaattcatg atgtactcaa
      961 aaaaaacaaa cgaaatatgc ctataaaatt agggttccct gcttgaggct gccagcggtt
     1021 atctttccat gacatcagct ttgcaagtgg gtaggcacgt aacccattat cattaggggc
     1081 aaccttgcca gtgtgataag ccacataacg gcccatccaa atcattaaat gctgctcttg
     1141 accttggtca aacaaaagca agtcggctac ttccgcatgc gccatatcac ggccaatagg
     1201 ctgtgtgttg ttttgcacaa gctctagcgc gctgacaaat gccccttggc taccatctgc
     1261 tctcagccag gcatggcgca gagctgcgcg ttgctctacc gttaagttga gttctggcgg
     1321 aatttttttt cccaaaaaac cattggcttt taaccaagta gcatcatgct ggcgtagcgc
     1381 ctctgcactt gcaaagcgta ctaagccagc acagtcgcgt tgttgccagc gcggtgtggg
     1441 gccaagattt aattgctgat gcacaatgag ttgtagccaa ctacgaaaag cctgcatttg
     1501 gcttgtattg aggcgcactt gattgctatt acgctgatta gacggataac ccaaacttgg
     1561 tcgctcatcc tttgacatca atgcaaaaac ttgttttggc gatgccagta ctaacaacag
     1621 cgcagcaaat ccacccaaaa caccgcgacg accatggctt ggctggtgtt ttgcaatcat
     1681 caaatctgat ttaaccgtct gctgctgcat atcattttac cgttcagggg cagtacgcca
     1741 gacaagtggt tgccattgcg caatgtcatg cgcactgctg cctgcctgtt tggttttaga
     1801 agcctttgat tccaaaacca agctaaacgg acgctgctgg gatagtgcag caaagcgagc
     1861 aggcatatgg taatcaaacg ctgcccttaa attgcttttt gtttcttgcg gtaatgccgc
     1921 atggcccgtc gcggtgagca gtgtggaaag tttttttgga ttcacataaa gaaattgctg
     1981 tgttgattgt tgcaacacaa ctgctgactc ctgcaaattt gggtattttt tctcgccaac
     2041 actgactgca cgctgagtta acgcagggtt cgttgaaaag tagaccactt cattatcaaa
     2101 agcaagtaca gggttttttt ctgaagtagc ctctatggaa agtttgcgtg acaccacggt
     2161 aaaagcaccc tgttgaacga ttgcgttttc ttttgcaatc agtgctggcg cttcggcagc
     2221 ctgttttacc gcggtgatgg cgggcgcaag ttcggctagg gctgtttcgg ccactttaac
     2281 ctccgctttt gaggattcaa tagcagcttc tttttgcgct tgtcgtgccg ctttctgctc
     2341 gcggtctagc ttgccgtcaa ttttttcttg cttagctgct tcaagattgg atgctgcata
     2401 ttccatctgg ttttttaaat gacgtttttg gcgattgagc gctattaacg ctttcagatg
     2461 gtcttgattg gttgcaacgc cccagtcaaa aagcgctgtc agcatttctt gtttcttctg
     2521 gctcggctgg cgtagcgcta caaataaggg ctgggcaatg tcatcacttt tttcggcata
     2581 ccaacaaatt gcaccactgg atgcaaattc agcaggcaaa ttcaattggg tgttcaaatt
     2641 ttttgcaccc gccagagcag tttgtacttg cgcccaatca attggtgtag ctacgcaaaa
     2701 cgcggcattg gcaggcaaat gcctccaaat attgggatta aatgtattgt cagcaatatt
     2761 agcttgggtt tgccattgtt taccgtcaaa atcaaagcgc aatgcgccca aatagggaac
     2821 aaaaggcgcg tatccttgcg caaacaagcg gttagaaact aaaatcgtct gcttagattc
     2881 atgcacaggc tgtgcattcg cggtaacttg ccattcagca atgacttctg cccgcacttt
     2941 ggaatcttca gacagcaggc gcgaaataag tttcgctgtc tggtcgtcaa gttcattatc
     3001 agcatggctt accatgtcga tgtctgacag cagcaccagc cgattttggt gtgctgccaa
     3061 aaccatttga cggcgtgatg agagcgacaa ttgcagtacg ggaacgctcg tcccagcaat
     3121 acgcacactg ccaatttgct gcaattgctt atccgcagtg agttttagtg tcgctaattt
     3181 ttgtgcgact gcgcttaatt gatcacgctc taaactaagc gcccaatagc gcaaggcgtg
     3241 gctatcatcg tgccacatgt aaatatcggc tggcgcttct gcaatatttt taagtagggt
     3301 gtctgaccag ttcagtgcat gctcaaaact aatgcggcgc atggcgcctt gcaaactgag
     3361 ccaatcttca tcttccgcat aaaaatatac aaaatcttcg gtcaacaatg ttttaagcaa
     3421 cggcacttgc agtaaatcgc ggggtaataa tgataggttt tggctgtgca cccacacatc
     3481 aggctgcgct aacgccacgc cattttggct attttttgcg agtggaattt taaaaaaaat
     3541 accggctaaa atggcacccg ctaataaaaa accagcaatg agccaagttt tttttctgtg
     3601 agaaaatgac tgttttacct tttcaaacaa tgaagatgaa ggcggtgctg acgcattgct
     3661 attattgttg ttcgattgca tggtttgatt gcttagtgta atggccaatt agaatgtttg
     3721 ataatgcgtg aatgcacaat attagtcaat ttttgccagg ccaatatttt tttcgtctgt
     3781 gcctgttatt cacgtattgt cgagattgca cgctcaatga tactgagcgc ttcttcaaac
     3841 actgcatctt gaatggtgag tggatacaaa aatcgaaccg cattcatata cacgccgcag
     3901 gttaatagta ccaagccttc tgccagcgcg gcctgttgca cgcgttttgt catttctggt
     3961 gaaggctgtt gcgtgactgg gtcaaaaaat tctgctgcaa tcatcgaacc caagccacgt
     4021 acttctttaa gagccggcgt attcacttgc atagccttta atctgctggt gagcttggcg
     4081 cctaaaacat tggcacgcgc aaccaattgc tcttcttcaa tgatatcaat cacagcatgg
     4141 gctgcagcaa tagctaacgg attacctgca taggttccgc ccaaaccacc gggcgctggg
     4201 ccatccatga tttcagcctt accacaaaca gccgagagcg tcataccgcc tgccaagctt
     4261 tttgccatta ccataatgtc gggggtcaca ttaaaatgtt cggctgcaaa taattttcct
     4321 gtgcggccaa acccggtttg cacttcatca aaaattagca aaataccgtg ctcatcacac
     4381 aaggtacgca gtgcattcat gagcgcaggc ggtgtgacat aaaaaccgcc ctcgccttgt
     4441 attggctcaa taataatcgc ggccacacgc atagggtcaa catctgcctt aaaaagtgtt
     4501 tgtattgcgt gtagcgcatc ttcaacgctc acgccatgca actccgtagg aaacggcaca
     4561 tgataaatct cagcaggaaa cggcccaaac cctactttgt aaggcaccac ctttcctgtg
     4621 agcgcgcaac ccatcatggt gcgaccatga aaagcacctg aaaacgcaat aacacctgaa
     4681 cgattggtgg cagcacgcgc aatttttatt gcattttcaa ctgcctctgc cccgcttgaa
     4741 aaaaagcagg ttttttttgc atgactacca gccgttaatt gattgatgcg ttcagccaat
     4801 gtcacataat tttcataggg caacacctga taacaagtat gggtaaattg ctgcaattgt
     4861 gcttcaacgg cggcgaccat tttagggtga cgatgcccgg tgttgagcac tgcaatcccc
     4921 cctgcaaaat caatatagcg cctaccttca acatcccata cttcagaatt tagcgcgcgt
     4981 tcgataaaaa aattggccat cacaccaatg ccgcgcggtg tggcggcaag acggcgctga
     5041 tgtaaatctt gattgctggg cggttgcgtt gtcatattaa aaaaccttca ctcaataaat
     5101 gcaatttctc atttttatct gccgttttaa tgcaatatgc gggcttaaaa atctacgaga
     5161 gaaaaaatat gctctggaag cctttgttta taaggcttcc agagcatcga aaaaggttgg
     5221 tgctaaattg ccacttaaaa caacttattt cagccgtatc catgtggtct tcaattctgt
     5281 gtatttttct agcgcatgca gtgatttatc gcggccattt ccactttgct tgtagccgcc
     5341 aaacggcaca gtaatgtcgt cttcatcgta actattgaca tgcaccgtgc ctgcgcgtaa
     5401 ccgctgtgcc accaaatgcg cgcgcgagat atcctgcgtc caaatcgcgg ctgctaagcc
     5461 atagggtgtg tcgttggcta ttttgatggc ttcttcttcg ctatcaaagg caataataga
     5521 caatacaggg ccgaaaattt cttctgccgc aatcgtcatg tcatttgtca ccgcatcaaa
     5581 cacggtcggg ctaatataaa aaccgcccgt ttcagtgcgc acttgcgcac cacctgcgag
     5641 tagttttgcg ccctgcgctt taccactggc aatgtatttc agtacatttt ccatctgccc
     5701 acagtccaca attgcgccca tagtagtttt ctcatccagc gggtcacctg gctgataatc
     5761 aggcaaatgc gcaagcaact ttttaacgaa cgcatcttta atagcgcgct caaccaataa
     5821 gcgtgatggc gcattacagc tttcaccttg gttgtaaaaa attgagccaa ttgaagccgc
     5881 tgcggcctta tctaaatctt tgcaatcggc aaatacaata ttgggtgact taccgccaag
     5941 ctcgcaccaa gcccgcttta agttgctttg cccagccatg atttgaattt ttttgcccac
     6001 acctgtagag cccgtaaacg caatgcaatc aacatccatg tgcattgcaa gcggtgtgcc
     6061 tgcttgctcg ccaaagccag ggatgatatt caacacgcct gcgggaatgc ctgcctctaa
     6121 tgccaaatct cctagcctca gcgcggtgag cggagatttt tctgaaggtt tcaacacaac
     6181 gctgttacct gacgctaaag caggcgcaat tttccaacac gccattaaaa tcgggtaatt
     6241 ccaaggcaca atcgccccca ccacacccat cggttcgcgc gtaatcatgc ctaacgtgtt
     6301 atctgccgtg ggtgcaattt catcgtacac tttgtcaatt gcctcgccaa accagcgtag
     6361 cgaattcgcg gctgaattca catccacact caaactggca gaaatgggtt tacccatatc
     6421 gagcgtttct aacaaggcca attcttctct attggtaatg agtaaatctg caaagcgaat
     6481 catggcttgc ttacgttcac gcgggctcaa accagcccat cggcggtcat taaacgccgt
     6541 gcgcgcgcag gccacagcta aattcacatc aatttcttgg caactggcta catcggctaa
     6601 tttgcgatta tcaatcggcg aaaaacattc aaatgtttgg ccgcctgccg caggcatacg
     6661 cacaccgcca ataaaagcac ggccgtcaat ttttaaggtt tttgcacgtg cgtgccagtc
     6721 tgttgttgta tcagccatat tgtttaaagc cccgccattg caatatattt aatttctaaa
     6781 tattcatcta taccgtggtg cgaaccttcg cgccccaagc cagattgctt cacgccgcca
     6841 aacggcgcca cttcgttgga aatcatgccc gtattcacac ccaccatgcc atattccaac
     6901 gcttctgcca cgcgccaaat gcggccaata tcgcggctat aaaagtatga ggccaagcca
     6961 aattcagtat tattggccat tgcaatcaca tcttcatccg ttttaaagcg gaataatggc
     7021 gcaactgggc caaaggtttc ttctttaaaa attaaggcat tcgggctcac gtctgcaagt
     7081 acagtgggtt cataaaacgt gcccccgttt gcatgacgtt tgccgccttg taataaagtt
     7141 gcgcccttgg ccaccgcatc tgccacatga ctttccactt tttcaactgc cgcattatca
     7201 atcagcgggc cttgcgtcac accttctgcc gtgccatcac ctacttttaa tgtggccacc
     7261 tttgccgcaa gcttagctgc aaacgcatca aacacgccat cttgtacaaa caagcggtta
     7321 gcgcatacgc aagtttgccc ggcgtttctg aacttgctca ccatcgcgcc ttcaaccgcc
     7381 gcatctaaat cagcatcgtc aaacacaata aaaggtgcgt taccgcctaa ttcgagtgat
     7441 aattttttaa tcgtatccgc acattgacgc attaaaatgc gcccaacttc ggttgagcct
     7501 gtgaatgaca acttggttac aatcgggctt tcacacagca ctgcgccaat ttcacgcgcc
     7561 ttacccgtga gcacttgcaa cacgcctgca ggcacgccag cttcttctgc caacacggct
     7621 aatgcgattg cagataacgg cgtattttct gcgggcttga taatcatgct gcagcctgcg
     7681 gccaaagcag gtgcggcttt acgggttatc atcgccacag gaaaattcca cggtgtaatc
     7741 gcagcggtta caccgatagg ctgcttaatc acaagcagtt tacggtcagc tgtgggtgct
     7801 ggaatcactt caccatatac acgcttagct tcttcagcaa accactccac aaagcttgcg
     7861 ccataagtaa tttcgccacg cgcctctgcc agcggcttac cttgttcggc agttaaaatt
     7921 tgggctaaat cttcatgatg ttccaccatt aaatcaaacc agcgtttaag aatagcagcg
     7981 cgcgctttgg ctgtaagcat tttccactgt ttttgggcgg tttgactggc aataatcgca
     8041 cgctcagttt cggctttacc catgcgaggt acattggcga tggcttgccc attagcagga
     8101 ttagtcacag tgaaagtttc gccagaatct gcacctaccc aattgccagc gagtaaggtt
     8161 tggttatttt tgattaaaga cttgtgttta atttgaagcg tcatgatgct ctctaattcg
     8221 cagtgtaggg gcgcaaatca ttgcacccca aagttattta ttactatcgc tatacgcctg
     8281 cgcttcttca cgtttacgtt tgcgcgcttg gtgcgaggta taaaagcttg aagcaatcac
     8341 cgcaaaagtc accaccacaa tcgtcaccgt cgccaccgcg ttaatggttg ggttcaagcc
     8401 taagcgtgcg cgtgaaaaaa tcaccatcgg catggtggaa tatcctgggc cagataagaa
     8461 cgatgacagc accacatcat caaaagacag tgtaaacgtt aataaaaagg cagaaattaa
     8521 cgctggtaac agcaatggca aggtgaccaa ataaaacact tgccaaggtt tacagcctaa
     8581 atccatggcg gcttcatcta atttactatc catttcgcgc aaacgcgaag tgaccaccac
     8641 cgctgcataa gcggtgccca acaaggcatg gcctaataaa atggtaaata aacctttttc
     8701 aggaaaaccc aaccaatgtt gcaccgagat aatcattagc aacaacgat
//
LOCUS       DAOQSI010000082         8718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_7541_length_8718_cov_5.921967, whole genome shotgun sequence.
ACCESSION   DAOQSI010000082 DAOQSI010000000
VERSION     DAOQSI010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 8718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8718
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7541_length_8718_cov_5.921967"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PKL42_06420"
     CDS             <1..741
                     /locus_tag="PKL42_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66749.1"
                     /translation="DDRASNPNANTVLKLTELSIGGDPQPITVAPTLSEINGKRAVFV
                     GTGKYIESSDLLPANYRQQSLYAFTDYGITSTLSGAALRALMEPQTISGGSGTRTSSG
                     NSPDWVAKIGWYADLPSSASTGERENVEATIISGKLFVPTLIPPTGTCDVSGKAWTNI
                     FDFKTGALTSSTESASPIAGLYFVFTNPTDDKVAVGTDFADGRPQNTDLTGLDAGGNT
                     VYKPHREDLLGTNGAFVGNRAIWRELVE"
     gene            complement(901..2025)
                     /locus_tag="PKL42_06425"
     CDS             complement(901..2025)
                     /locus_tag="PKL42_06425"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNU66750.1"
                     /translation="MSNITQSIKSLFSKNKVLIFALVVSLLFHFLLFSKFYFSFPKQT
                     NNQQTLNVRLANVQSKQKTSSKPNLETSTSSLNIPKPLQTQPPLAVANEITEIVEKSL
                     ELHEEPLMPTETTPIPALQEALSNEQEIIDITDHAAISNEPLNSAEQELIEATKVNAT
                     QPYKYVETEFEITQNNELHSSGIGRTIFNLDNNQTYTLISTIQTNRPTALNLNILRQT
                     SEGIVTENGLVPSYYSSHYSNEPSKAKSARFFWPDGALLLQDSKSEKTEPLNADTQDL
                     LSFMYQFMFAPPKNNGEITVTNGENSQSITYTSLGEEVITSNIGELKTIHLLINSLGQ
                     IKTEIWLAIDYQYLPIKIRKTLNNGLVIEENAVSIYTPQS"
     gene            complement(2046..2564)
                     /locus_tag="PKL42_06430"
     CDS             complement(2046..2564)
                     /locus_tag="PKL42_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HNU66751.1"
                     /translation="MNVKHIQTTVEDHQVAKLAAFAIALHMIEAVIPSPLPGVKPGIA
                     NIITLFVLYRYGFATAAWVSILRVFASSLLLGQFLSPAFSLSLGGAVVSLVVLWLAAH
                     LPSRHFGPVSLSILAAFGHIAGQLIIVRLWLIPHAGISYLIPIFATAALFFGLINGLI
                     TAKLINVKPSNN"
     gene            complement(2581..2907)
                     /locus_tag="PKL42_06435"
     CDS             complement(2581..2907)
                     /locus_tag="PKL42_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HNU66752.1"
                     /translation="MSIIGIIWLFNTLWSNEQAAKVQIRQGEKIIGTYSLNQAREVQI
                     KGPIGVNTIHISNGRARFSKSPCHNQYCVHQGWLNRAGQAAICLPNQISLELIGETKP
                     YDSLNY"
     gene            complement(2954..4033)
                     /locus_tag="PKL42_06440"
     CDS             complement(2954..4033)
                     /locus_tag="PKL42_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNU66753.1"
                     /translation="MRKVILYAAVIAVIGFGILQFKPKTPLYHSQSYVFGTLVDISIY
                     GETDERARAVTNLILQDFQHLHHRFHAWKTDTSGHASELGQLNEAFAKGTSPIEVQAD
                     LANIIRDATALSVKSNGLFNPAIGHLVSAWGFQRDEFSAVQINQEAINNLVQAAPRMT
                     DIVLNEKNVYSKNPSVKLDLGGYAKGYALDLALQRLRQEKINHALVNIGGNIIALGQH
                     GQKPWRVGIQHPRAPTAIATLDLADGWAIGTSGDYQRYFFKEKQRYCHIIDPRTGYPV
                     QHTQSVTVLIPPQSAHQIQAGVLSDVATKPIFIASPENKNQMAKKFGIEYLMVIDEKS
                     NILISKKLAKNLHWQQPDVQFSTFE"
     gene            complement(4034..4984)
                     /gene="gshB"
                     /locus_tag="PKL42_06445"
     CDS             complement(4034..4984)
                     /gene="gshB"
                     /locus_tag="PKL42_06445"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HNU66754.1"
                     /translation="MKLLFILDPLAHLKTYKDTSLAIMREAANRGHTLYVSMQHDLFL
                     RGSTARINAKIFNFTAQDFTTSEPNEFSPADFDAVIMRKDPPFDNEYLYSTYLLEIAA
                     SQGARVYNNPAAIRNWNEKLSVTRFAEFTPAFLVTANQQRIREFLNDQQDIVVKPLDG
                     MGGSSVFRLTLTDPNISVILETITQFGTRTVMAQRYLPAITQGDKRIIMINGEPIPYA
                     LARIPKAGETRGNLAAGGKGVAQLLSARDVEISTAIGKVLKQEGLLLVGLDVIGDYLT
                     EINVTSPTGMVEIAQQTAHLAEPCNPASIMLDALAQLAQT"
     gene            complement(5065..6387)
                     /gene="gshA"
                     /locus_tag="PKL42_06450"
     CDS             complement(5065..6387)
                     /gene="gshA"
                     /locus_tag="PKL42_06450"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HNU66755.1"
                     /translation="MVPHLTTALNGPLLGLEKKMLAAQPKIEHWFRTQWLDYAAPFYA
                     SVDLRNAGFKLAPVDTNLFPGGFNNLNPVFLPLSVQAATVAVEKVCPEAHRLLIIPEN
                     HTRNTYYLRNVVELVHIMKAAGLDVRVGSISPEITQPTTFETHDGQHLLLEPVVRVGN
                     RIKLINPELGDFDSCAILLNNDLSAGIPEILKNLEQNLIPPLHAGWSTRRKSQHFSAY
                     NRVVADFAKLLEIDEWLLNPYFETCGEINFHERTGEECLALKVAQLLEKIKIKYAEYG
                     VTQEPFVIVKADAGTYGMGIMTVKSPDDVRDLNRKARNKMSVIKEGLQVSEVIIQEGV
                     YTFESINSAVAEPVVYMMDHFVIGGFYRVHTGRGIDENLNAPGSQFVPLAFEEPCTMP
                     DCADAPDAAPNRFYAYGVVARLALLAAAIEIQEQDPFNQEDAQLAISI"
     gene            complement(6445..7401)
                     /gene="lipA"
                     /locus_tag="PKL42_06455"
     CDS             complement(6445..7401)
                     /gene="lipA"
                     /locus_tag="PKL42_06455"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNU66756.1"
                     /translation="MAETNITNRKVAGVKERDAAKTSRIPIKIIPQPIQRKPEWIRMK
                     VPDSARYQEIKKILRDNHLHTVCEEASCPNIGECFSGGTATFMILGDICTRRCPFCDV
                     AHGKPLPPDVNEPENLARTIAQMQLRYVVITSVDRDDLKDGGAQHFVDCIQAVRRLSP
                     NIKIEILVPDFRGRLDVAMEILRNAPPDVMNHNLETVPRLYKQARPGSDYQNSLSLLK
                     KFSEMYPEIPTKSGLMLGLGETDDEIISVMQDLRAHRVSMLTLGQYLQPSEHHHPVMR
                     YVEPAIFDTLKQKAEAMGFNNTASGPMVRSSYHADVQAHLVK"
     gene            complement(7546..8175)
                     /gene="lipB"
                     /locus_tag="PKL42_06460"
     CDS             complement(7546..8175)
                     /gene="lipB"
                     /locus_tag="PKL42_06460"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNU66757.1"
                     /translation="MHLPIHIRRLGVTDFESTWHAMQQFTATRTAETPDEIWLTEHYP
                     VYTLGLNRKEVRLPSLAHIPLVMTDRGGKITYHGPGQLIVYILLDMQRYQLNVRKLVS
                     LLEQAVVELLAKDGIAAVAKSDAPGVYVADAKIASLGLRIKNNCCYHGLSLNIHMDLS
                     PFEQIDPCGYVGLKVTQTHDLKSELNLENAGEFLVKKLTQQLQTFFDAL"
     gene            complement(8181..8474)
                     /locus_tag="PKL42_06465"
     CDS             complement(8181..8474)
                     /locus_tag="PKL42_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HNU66758.1"
                     /translation="MAKSENLANQGAKTPLIDFPCDFSIKVMGETQESFAQTIIEAIQ
                     TKEPTFNASKVEMRASSGGKYISLTCSVYVTSQTQLDDIYRVISGHPLVKFAI"
     gene            complement(8487..>8718)
                     /locus_tag="PKL42_06470"
     CDS             complement(8487..>8718)
                     /locus_tag="PKL42_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HNU66759.1"
                     /translation="QDYLITLPKGEYNNVKQVISSTQPLIAPIKKGQVIGNVKFTLNG
                     KVIDERPLVAAKSVEIAGIFGRAWDSIKLLLQ"
BASE COUNT         2307 a         1812 c         2021 g         2578 t
ORIGIN      
        1 gatgacagag ctagcaatcc aaatgctaat accgtactta aacttacaga attatctatt
       61 ggtggtgatc cgcagccaat tactgttgcg cctacgttga gtgaaataaa cggaaaacgt
      121 gcagtttttg taggtactgg caaatatatt gaatctagtg atttgctccc tgcaaattat
      181 aggcaacaaa gcttatatgc ctttaccgac tacggtatta cttcaacgtt gtctggcgcg
      241 gcactgcgcg ccttaatgga accacagacg atctctggtg gtagtggtac aagaacaagc
      301 tctggtaaca gccctgattg ggttgccaaa atcggctggt atgctgattt acccagcagc
      361 gcatcaactg gtgaacgtga aaatgttgaa gcgactatca tttcaggtaa gttatttgtt
      421 ccaacgctta ttccacctac aggcacatgt gatgtaagtg gtaaggcatg gacaaatatc
      481 tttgatttca aaaccggggc attaacgtca tctactgagt cggcttcacc aattgccgga
      541 ttgtattttg tcttcactaa tccaacggac gataaagtgg ctgttggtac tgattttgca
      601 gatggaagac cacaaaatac tgatttgact ggccttgatg caggcgggaa tactgtttat
      661 aaacctcatc gcgaagatct gcttggaacc aacggtgcgt ttgttggaaa cagggcaata
      721 tggagagagt tagtggagta aatgctttaa actagactaa aagccacggt aaagtcaaac
      781 ttaccgtggc ttttttctac ctaagtgttg ttggcgagta ttttagttat tcagagttat
      841 aactgaatat ccggtttgtt ggaaattaga gaagtgaaat aacgcgcagt aaaaacacaa
      901 ttaagattgt ggagtgtaaa tactgacggc attttcttca ataacaaggc cattattaag
      961 tgttttgcga attttgatag gtaaatattg gtaatcaata gcaagccaaa tttctgtttt
     1021 tatctggcca aggctgttaa ttagcaaatg gatggttttt agttcaccga tgttagatgt
     1081 gatgacctct tcacctaatg atgtataggt gatgctttgc gaattttcgc cgttagtaac
     1141 cgtaatttcc ccattgtttt ttggtggcgc aaacataaac tgatacataa agcttaataa
     1201 atcctgcgta tcagcattta atggctcagt cttttcactc ttactatctt gcaatagtaa
     1261 agcgccatca ggccaaaaaa acctagcact tttcgctttg ctcggttcgt tcgaataatg
     1321 gctggagtag taactcggaa ccaagccatt ttccgtcact atgccttcac ttgtttgcct
     1381 caagatgttt aagttgagtg cagttggcct attggtttga attgtgctga ttaatgtata
     1441 tgtctgatta ttatctagat taaatatagt acggccaatg ccagatgaat gtaattcatt
     1501 gttttgggta atctcgaatt cggtttcgac gtatttgtat ggttgagttg catttacctt
     1561 agttgcttca atcaattctt gctcagcact attcagaggt tcgttggaaa tagctgcatg
     1621 gtcagtgata tcaatgattt cctgctcatt cgatagggct tcttgcagcg ctggaatagg
     1681 tgtggtctca gttggcatga gtggttcttc atgtagttct agcgattttt caacaatttc
     1741 cgtaatttca ttcgctacag ctaaaggtgg ttgggtttgt aacggttttg gaatattcaa
     1801 tgaggatgtg gatgtttcaa ggtttggttt ggatgaggtt ttttgctttg attgcacatt
     1861 ggctaaacga acattaagcg tttgttgatt atttgtttgt ttaggaaaac taaaataaaa
     1921 cttagaaaat aataaaaagt gaaataacaa ggaaacaaca agagcaaaga taagtacctt
     1981 gttttttgag aataaagatt ttatggactg ggtaatattt gacatgtgat atcagtattg
     2041 cacttttagt tattactggg cttaacattg atgagtttag cagttatcaa gccattgatt
     2101 aacccaaaaa ataacgcggc tgttgcaaat attggaatta aataactaat ccccgcatgc
     2161 ggtattagcc ataatcgcac gataatcagt tgccctgcaa tatgcccaaa agctgccaaa
     2221 atactcaggc ttacagggcc gaaatgacga ctaggcaagt gggcggccaa ccataaaaca
     2281 actaaactaa cgactgcccc gccaagactt aaactgaaag cgggggataa aaattgacct
     2341 aatagtaaac tgcttgcgaa aacccgcagt atgctgaccc aggcagcagt tgcgaagccg
     2401 taccgatata acacaaatag agtaatgatg ttggcgatac ctggctttac accgggtaag
     2461 ggggatggaa ttactgcctc tatcatgtgt agcgcaatgg caaaagccgc aagcttggca
     2521 acttggtggt cttcaaccgt ggtttgaatg tgtttaacgt tcaatttata tggaaatcaa
     2581 ttagtagtta agtgagtcat aaggtttggt ttcaccaatt aattctaggc taatctggtt
     2641 aggtaaacaa atggcggctt gccctgctcg gtttagccag ccttgatgga cgcaatattg
     2701 gttgtgacac ggcgattttg aaaatcttgc gcggccattc gaaatgtgaa tggtgttgac
     2761 gccaattggc cctttgattt gtacttcacg ggcttggttt aaactgtaag tgccgatgat
     2821 tttttcaccc tgccgaattt gcacttttgc tgcctgttcg ttagaccaca gtgtgttgaa
     2881 taaccatatg atgcctatga tgctcatcag gatgagtatg ctatcaccta ggcgcaggta
     2941 ttttgttagc tggttattca aacgtagaaa attgcacatc aggttgctgc caatgcaggt
     3001 tttttgccaa tttttttgaa atcaaaatgt ttgatttctc atcaatgacc atcaagtact
     3061 caatgccaaa tttttttgcc atttggtttt tattttcagg tgacgcaata aaaatgggct
     3121 tggtggcaac gtcagataat acgcccgctt gtatttgatg cgctgattgt ggcggtatta
     3181 gcacggtgac agattgtgta tgttgcacgg gataaccggt tcgcgggtcg atgatatggc
     3241 aataacgttg cttttcttta aaaaaataac gctgataatc gcctgatgta ccgattgccc
     3301 atccatcggc taaatccagc gtggcaatgg ccgttggcgc gcgcgggtgc tgtatgccaa
     3361 cccgccaagg tttttggcca tgttgaccta acgcaataat attgccgccg atattcacga
     3421 gcgcgtgatt tattttttct tggcgtaacc gctgcaatgc taaatctagc gcgtaacctt
     3481 tagcatagcc gcctaaatca agcttaacgc tagggttttt actataaaca ttcttttcat
     3541 tgagtacgat atccgtcata cgaggcgcag cttgcacaag gttattgatg gcttcttggt
     3601 taatttgcac tgcgctaaat tcatcacgct gaaatcccca tgcacttact aagtgaccaa
     3661 tggcagggtt aaataatcca ttagatttta ccgataatgc agttgcgtca cgaataatat
     3721 tggctaagtc tgcttgtact tcaatgggtg aagtcccttt tgcaaaggct tcatttaatt
     3781 gccccagctc gctggcatgg ccagaggtat cagttttcca agcgtgaaag cggtgatgta
     3841 agtgctgaaa atcttgcaat atcaggttag tgactgcgcg tgcgcgttcg tctgtttcgc
     3901 cataaatgct gatatctacc agcgtgccaa acacatagct ttggctatga tataacggcg
     3961 ttttaggttt aaattgcagt atgccaaaac caatgaccgc tatcacggcc gcatacaaaa
     4021 tcactttgcg catttatgtt tgcgccaact gcgctaaggc atcgagcatg atgctggcag
     4081 ggttgcaagg ttctgccaaa tgggcggttt gctgcgcgat ttcgaccatg cctgtcgggc
     4141 tggtgacatt gatttcagta agataatcgc caattacatc caagccaact agtagtaaac
     4201 cttcttgttt aagtactttg ccgatggcgg tgctgatttc aacatcacgc gcacttaaca
     4261 gttgcgccac gcctttgccg ccagccgcaa gattgccgcg tgtttcacct gcttttggaa
     4321 tacgtgcaag tgcgtaaggt atgggttcgc cattaatcat gatgatgcgc ttatcccctt
     4381 gtgtgatggc aggcaaatag cgttgcgcca ttacggtgcg tgtaccaaac tgcgtgatgg
     4441 tttctaagat aacactgatg ttggggtcag ttaacgtgag cctgaaaaca ctgctgccac
     4501 ccatgccatc gagcggtttt acaacaatat cttgttggtc atttaaaaat tcgcgtatgc
     4561 gctgctgatt tgcagttact aaaaatgcgg gtgtaaattc agcaaagcgg gtgaccgata
     4621 gtttttcatt ccaattgcga atcgcggcag gattattgta tacacgtgca ccttggctag
     4681 cagcgatttc aagcaaatag gtactgtaga gatattcatt atcaaaaggt gggtctttgc
     4741 gcatgatgac ggcatcaaag tctgcgggtg aaaattcatt aggttcgcta gtggtgaaat
     4801 cttgcgcagt aaaattgaat atcttggcgt taattctagc cgtgctgccg cgcaagaaaa
     4861 ggtcgtgctg catactgaca tacagcgtgt gaccacggtt tgccgcctca cgcatgatgg
     4921 caaggctcgt gtctttgtac gtttttaaat gtgcaagcgg gtcaagaatg aaaagtagtt
     4981 tcatcgggct attctaactt tatcgcgact atttagccaa taaaggcact tgattcatct
     5041 aaagtgacca attgccttca acacttagat ggagatggcc aattgagcgt cttcttggtt
     5101 gaaagggtct tgttcctgaa tttcaatggc ggccgccaac aacgccaaac gcgccacaac
     5161 gccataagca taaaagcgat tgggcgcagc atctggggca tctgcgcaat ccggcatggt
     5221 acagggctct tcaaacgcga gcggcacaaa ctgtgagccg ggagcattga gattttcatc
     5281 aatgccgcgt ccagtatgga cgcggtaaaa gccaccaatg acaaagtggt ccatcatgta
     5341 aacaacaggc tctgccactg cgctattgat gctttcgaac gtgtaaacac cttcttgaat
     5401 aatcacttca gagacttgta agccttcttt aatcacagac attttgttgc gcgctttgcg
     5461 atttaaatcg cgcacgtcat caggcgattt tacagtcatg atgcccatgc cgtaggtgcc
     5521 tgcatccgct ttaacaatta caaagggttc ttgtgttacg ccatattctg cgtatttgat
     5581 ttttattttt tcaagcagtt gcgccacttt cagcgctagg cattcttcac ctgtgcgttc
     5641 atgaaagtta atttcgccac aggtttcaaa ataggggtta agcaaccatt catcaatttc
     5701 gagtagtttg gcaaaatcag cgactacgcg attatacgca gaaaaatgtt gcgattttcg
     5761 ccgcgtgctc caacccgcat gtaatggcgg aataagattt tgctcaagat ttttaagaat
     5821 ttctggaatg cctgcggata agtcgttatt cagtaaaata gcgcagctat caaaatcacc
     5881 taattctggg ttgattagtt taatacggtt gcctacgcgc accacgggtt ctagcagcag
     5941 atgttggcca tcgtgtgttt caaacgttgt gggttgcgta atttcgggtg aaatactgcc
     6001 aacacgtaca tctaaccccg ccgctttcat gatgtggacg agttcaacca cgttgcggag
     6061 ataataagta ttgcgggtgt gattttcagg aataatcagc aggcgatgcg cttcagggca
     6121 aactttttca accgctacgg ttgcggcctg aacgctgagc ggtaaaaaca caggattaag
     6181 gttattaaaa ccaccaggaa ataaattggt atccaccgga gcaagcttaa agccggcatt
     6241 gcgcaaatca acactcgcat aaaatggcgc ggcataatcg agccattgcg ttctaaacca
     6301 atgctcaatc tttggctgcg ccgccagcat tttcttttct aggccaagta gtgggccatt
     6361 gagggcagtg gttagatgtg gaaccataat ctctttctaa acaataggtt gctaagtccg
     6421 ctatctggtt ttctgggtta gcgattattt gactaaatgt gcttgcacgt ccgcatgata
     6481 gctacttctt accatggggc cactggcggt attattaaag cccatcgctt ccgctttttg
     6541 tttaagcgtg tcaaaaatag cgggttcaac ataacgcatc acagggtgat ggtgttcgct
     6601 gggctgtaaa tattggccaa gtgtgagcat gctcacgcga tgcgctcgca aatcttgcat
     6661 cacactgata atttcatcgt ccgtttcgcc caagcctaac attaaccctg attttgtggg
     6721 gatttctggg tacatttcac taaatttttt tagtaatgac aacgaatttt ggtagtcaga
     6781 acccggccgt gcctgcttgt ataggcgcgg cacggtttca aggttatggt tcatcacgtc
     6841 aggcggtgca ttgcgcaaaa tttccatggc aacatctaac cggccacgaa aatcaggcac
     6901 gagaatctca attttaatat tgggtgaaag cctacgcacc gcttgaatgc agtccacaaa
     6961 atgttgcgcc ccgccatctt ttaaatcatc tcggtcaact gaggtaatga ccacatagcg
     7021 aagttgcatt tgcgctattg tgcgggccag attttcaggt tcattgacat cgggcggtaa
     7081 gggtttgccg tgtgcaacgt cgcaaaatgg gcagcggcgg gtacaaatgt cgcctaaaat
     7141 cataaaggtg gcggtaccgc cactaaaaca ttcaccaata ttcgggcaac tcgcttcttc
     7201 gcacaccgtg tgcaggtgat tgtcgcggag gatttttttg atttcttgat agcgcgcaga
     7261 gtcaggcact ttcatgcgaa tccactcagg cttgcgctga ataggttgcg gaataatttt
     7321 aatgggaatc cgtgacgttt tggcagcatc gcgttctttc acgccagcga ctttacggtt
     7381 tgtgatgttt gtttcagcca tgcttaaatc cattattttt tatagataac gcgcgcttaa
     7441 aattgagcgc taaattgaca actcaagatt accgaaattc aacctaaatg tactcaaaaa
     7501 cacccgagaa attttttctg ccctgcgagc ctttatttat aaggcttaca gagcatcgaa
     7561 aaaggtttgt agctgttggg tgagtttttt taccaaaaat tcacccgcat tttcgaggtt
     7621 gagttcactc tttaggtcgt gcgtttgcgt cacttttaag cccacatatc cacaggggtc
     7681 aatctgctca aaagggctta aatccatgtg gatatttaaa cttaagccat gataacagca
     7741 attattttta atgcgtaacc caagggacgc gatttttgca tcagctacgt acacgcctgg
     7801 cgcatctgat ttggcaactg ccgcaatgcc gtcctttgcg agtaactcaa cgaccgcttg
     7861 ctctaatagg ctgaccagtt tgcgcacatt gagttgatag cgttgcatat cgagcaatat
     7921 atatacaatc aattgaccag ggccgtggta agtgattttt ccaccgcggt cagtcatcac
     7981 cagtggaata tgtgccaagc taggcaggcg aacttcttta cggtttagcc caagcgtata
     8041 aacagggtaa tgctcggtca gccaaatttc atcaggcgtt tcagcggtgc gcgtggccgt
     8101 gaactgctgc atggcgtgcc aggtgctttc gaaatctgtt acacctaagc gcctaatgtg
     8161 gataggcaaa tgcatgcggc ctagattgca aacttcacaa gtggatggcc gctaatgacg
     8221 cgataaatat catccaattg tgtttgtgag gtcacgtaga ctgagcaggt caggctaatg
     8281 tacttgccac cagaactggc gcgcatttct accttgctgg cattaaatgt tggttctttg
     8341 gtttgaatgg cttcaatgat ggtttgtgca aaactttctt gcgtctcgcc catgactttg
     8401 attgaaaagt cacaaggaaa atcaatgagt ggcgtttttg caccttgatt ggcgaggttt
     8461 tcagattttg ccatgacgct taaatgttac tgaagtagca gtttgattga gtcccaagcg
     8521 cggccaaaga tgccagcaat ttctactgat tttgcagcaa ctagcggacg ttcatcaatg
     8581 accttgccat ttaaggtgaa ttttacattg ccgatgacct gccctttttt aatgggcgct
     8641 attaaaggct gggtgctgga aatgacttgc ttcacattgt tatattcacc cttaggcagc
     8701 gtaatcaggt aatcttga
//
LOCUS       DAOQSI010000083         8622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_7662_length_8622_cov_5.134119, whole genome shotgun sequence.
ACCESSION   DAOQSI010000083 DAOQSI010000000
VERSION     DAOQSI010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 8622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8622
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7662_length_8622_cov_5.134119"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PKL42_06475"
     CDS             complement(<1..445)
                     /locus_tag="PKL42_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HNU66760.1"
                     /translation="MSQATNNKIFSNLWMIAVYLFLYLPIFTLVVYSFNDSKLISTWT
                     HASLRWYEALIKDDDLISAVLLSLKIAGISALMSVFFGTFTAFALNRYKRFKGRTLLS
                     SMSSMPLVMPDVIVGLSLLLMIISVQHWLGFPEKGLFTILLGHALL"
     gene            complement(445..1344)
                     /locus_tag="PKL42_06480"
     CDS             complement(445..1344)
                     /locus_tag="PKL42_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNU66761.1"
                     /translation="MATKQGFFTRLLDGKHAVIGIPYLWFILLAAVPILIILKISLSE
                     MQGTSVSNMIDWSNGWPKLTLNFSSYQFIFSDSLYLKTYLSSIKYALLTTLICLVIGY
                     PFAYFMARSPKHMQPTLLMLIMLPFWTSFLLRIYAWKTLLENNGIINNVLMSLGVIDA
                     PLQMMNTPFSLMIGMVYSYLPFMILPLYANLSKLDMRYLEAAADLGTSPFKAFWLITV
                     PLSKAGIIAGSMLVFIPTIGEYVIPELLGGSSTLMIGRVLWNEFFSNNDWPMASAVAV
                     VMILLILVPMAIYNKSQAEAEMK"
     gene            complement(1357..2478)
                     /gene="potA"
                     /locus_tag="PKL42_06485"
     CDS             complement(1357..2478)
                     /gene="potA"
                     /locus_tag="PKL42_06485"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HNU66762.1"
                     /translation="MATAPKEINQPAPEVLLRVENVTKIYDGTVKAVDDVSLTVNKGE
                     IFALLGGSGCGKSTLLRMMAGFEMPTKGKIFLAGQDITNLPPYQRPINMMFQSYALFP
                     HLSVWENIAFGLKRDGMPKDQIAERVEKMLNLAQLGKFAQRKPHQLSGGQQQRVALVR
                     SLAKRPQLLLLDEPLGALDKKLRETTQLELVNIIEEVGVTCVLVTHDQEEAMTMASRI
                     AVMSEGYFLQVGAPDAVYEMPNSRKVADFIGNVNLFDGVIEEDEADHVTVRCPECVHY
                     VGHGVSGHTGMAVGVALRPEKIMLSDKKPKGEYNWCEGEVIEIVYFGAHTTYHLKLDS
                     GKVIKAQELNNTRDLSCGLTWGDRAYAHWNDLAMVVLAQ"
     gene            complement(2558..3730)
                     /locus_tag="PKL42_06490"
     CDS             complement(2558..3730)
                     /locus_tag="PKL42_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HNU66763.1"
                     /translation="MQILLNKRLLCTMVGLAIVGFGSVSCTDSNKETTSDAKVAQSTV
                     ASEEEKILNIYNWSDYIAPDTIENFEKETGIKVRYDVFDSNEILHAKLIAKNTGYDIV
                     VPSSNWAKQQIEGGLFQKLDKTKLTNWKNLDEGILKQMEGVDPGNQYLVDWMWSYNTV
                     GINEDKVKAALGDTPMPDNAWDLVFNPTYTSKLKSCGITFLDTPTDVFQAALHYLGKP
                     VFTASNDDYQAAFDMLMKVRPHIKKFNSGGQIEDLAGGNVCVTYGWAGDINLARKRSI
                     ENKAEQNIVALVPKTGGLIFMDTMAIPADAKHPNNAHLFINYVMRPEVAASITNEVTY
                     ANPNKAATEFVDEEIKNNKSIYLSDEDIAKLVPPGVESQEAKRTLTRFYTKFKSGV"
     gene            complement(3905..5272)
                     /locus_tag="PKL42_06495"
     CDS             complement(3905..5272)
                     /locus_tag="PKL42_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNU66764.1"
                     /translation="MTNTKEIQAIDSAHYMHPFTDHKSLAAKGSRIITKADNVYIWDS
                     EGHKIFDAMSGLWCVNVGYGRKELVDAACKQMTELPFYNSFFQTTNVPAVKLAEKIIS
                     LAGDNYSHVFFGSSGSESNDTNVRMVRHYWATLGQPERTVIISRKNAYHGSTMVGSSL
                     GGMGGMHAQGGVISGVEHIEQPYHYGLAPDEDRDAFGLRAASWLEEKILEVGADKVAA
                     FIGEPVQGAGGVIIPPQTYWPEIQRICDKYGILLICDEVICGFGRLGAWFGSQRMGAK
                     PDLMTFAKGVSSGYIPLGGVVVGKRVAQVLIEQGGEFNHGYTYSGHPTACAVALANLE
                     IIEREDLVNQVRYETGPYLAQKYAELAKHPLVGGAETFGLVAGFVLMKNKEEHVHFDE
                     SLEVGMMCRGHCFANGLIMRAVGDRMIIAPPLVTTKAQIDEMIGLIYLCLDATLADLK
                     RLGVM"
     gene            complement(5296..6666)
                     /locus_tag="PKL42_06500"
     CDS             complement(5296..6666)
                     /locus_tag="PKL42_06500"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HNU66765.1"
                     /translation="MTEASAKNHIDMDKWLTDNNITEVECLVPDLTGVARGKILPREK
                     FSTERAMRLPEAVFGMTVTGESPEDNEGYDRVFGFTDKDMVLIPDPSTLRVVPWAQDP
                     TAQVIMDCFDHQGRPVNFSPRNVLRRVTNLYKELGFTPIVAPELEFYLLERNTDPDVA
                     LSPPVGRSGRKETSRQLYSIDAVNEFDPLFEDIYDYCDLMGLELDTLIHEFGAGQMEI
                     NFLHDEPMILADKTFFFKRTLREVAMRHGMYATFMAKPMQNEPGSAMHIHQSVIDCKS
                     GNNIFSKADGSASPLFFHYIAGLQKYLPAAMVLLAPYVNSYRRIVRNGAAPINIEWGY
                     DNRTVGIRVPISEPAGRRVENRVVGADANPYLAMAVTLACGYLGIKEKLQPTAETTGS
                     AYASDYQLPRGLSEAIRELEVATALHEVLGKDFVDVYLAVKDAEHDEFMRVISPWERE
                     HLLMHV"
     gene            complement(6725..7498)
                     /locus_tag="PKL42_06505"
     CDS             complement(6725..7498)
                     /locus_tag="PKL42_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNU66766.1"
                     /translation="MKNKQKKPVVLLPADVKPLGDHLFHAVGQKYILAVADAAEATPL
                     LIPAVADALDLEALLEMADGILLTGSVSNVHPSHFNQDVLDPSLPLDPARDALTLNLI
                     RTAIQKGVPLLAICRGFQELNVAFGGSLHQAVHDVDGLHDHREAKTVDLALQYAPVHS
                     VRLTEGGKLANITQSNEMFVNSLHGQGIHRLGAGLTIEATAPDGLAEAVSVDAAQTFA
                     LGVQWHPEWQVMQNPQYLAIFSAFGAACRLRMRRHEPPD"
     gene            7990..>8622
                     /locus_tag="PKL42_06510"
     CDS             7990..>8622
                     /locus_tag="PKL42_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66767.1"
                     /translation="MNKISQLTSMLFVASGLFFGLNPSAMAAPVVWTFNLPSTAVASQ
                     SPPYASVATLSLTQIGSDVQFFLDPNELNAGASPATSTFVHSIDFVYSGAVLTAANFA
                     YNAGASVDTFSYEVNQNNLDAGYTTQDQHVKVDFFSKNSDGGVRFDFTETSTWTIKDV
                     SLTNFTTTYATSGPKPSPIYGVISLGGFSGGTSNWVAAVPEPETYGMMLVG"
BASE COUNT         2458 a         2077 c         1718 g         2369 t
ORIGIN      
        1 ccaacaaggc atggcctaat aaaatggtaa ataaaccttt ttcaggaaaa cccagccaat
       61 gttgcactga aataatcatc aataataacg ataaacccac aatcacatca ggcatcacca
      121 atggcatact cgacatagac gacagcaatg tacgcccttt aaaacgttta tagcggttca
      181 gggcgaacgc agtaaatgtg ccaaaaaaca ccgacatcaa tgcagaaatg ccagcaattt
      241 taagggataa caacacggct gaaattaaat catcgtcttt aattaaagct tcgtaccagc
      301 gcaaactggc gtgcgtccat gttgaaatga gttttgaatc attaaacgag tacactacca
      361 gcgtaaaaat aggcaaatac aaaaacaaat aaaccgcaat catccacagg ttagaaaata
      421 tcttgttatt ggtcgcttgg ctcattattt catctccgct tctgcttgag atttattgta
      481 tattgccatt ggcactaata tgagcaatat catcaccacc gccacagcgg aggccattgg
      541 ccagtcgtta ttactgaaaa attcattcca taacacgcgg cctatcatca aggtgcttga
      601 gccgcccaat aactccggaa tcacgtattc accaatcgtt gggatgaata ccagcataga
      661 acccgcaatg atgcctgctt ttgaaagcgg cacagtaatg agccaaaacg ccttaaacgg
      721 gcttgtgcca aggtcagcgg cagcttctaa gtaacgcata tcaagcttag ataaatttgc
      781 atacaaaggc aaaatcataa aaggcaggta tgaatacacc atgccaatca tcagtgaaaa
      841 tggggtattc atcatctgta atggcgcatc gatcacgcct aaactcatca gtacattgtt
      901 gataatgccg ttgttttcaa gcaaagtttt ccacgcataa atacgtagta aaaatgatgt
      961 ccaaaaaggc aacatgatga gcatcaacag cgtaggttgc atgtgcttgg gtgagcgcgc
     1021 cataaagtag gcaaatgggt agccaatcac taagcaaatc agcgtggtga gtagcgcata
     1081 cttaatggat gataaatagg ttttaaggta gagagagtcg ctaaaaataa actgataact
     1141 tgaaaagtta agtgtaagtt ttggccagcc attactccag tcaatcatgt ttgaaactga
     1201 agtgccttgc atttcagata agctgatttt taatataatc agaataggca cagccgcaag
     1261 caaaataaac catagatatg gaatgccaat aacagcatgc tttccatcta acaggcgcgt
     1321 gaaaaaacct tgcttagtcg ccatttctcg ctccttttat tgtgctaata caaccatcgc
     1381 caaatcattc cagtgcgcat aagcgcgatc accccatgta agaccgcagc ttaaatcacg
     1441 cgtattgttg agctcttggg ctttaatcac tttgcctgaa tcaagcttga ggtgatatgt
     1501 cgtatgtgcg ccaaaataaa caatttcgat gacctcaccc tcacaccagt tatattcacc
     1561 tttaggtttt ttatctgaaa gcatgatttt ttcagggcgt aacgccacgc ccaccgccat
     1621 acctgtatgc ccgcttacac cgtgccctac ataatgcaca cattctgggc aacgtaccgt
     1681 cacatgatct gcttcgtctt cttcaatcac accatcaaat aaatttacat tgccaatgaa
     1741 gtcagccact ttgcggctgt tgggcatttc atacacggcg tctggcgcac ccacttgtaa
     1801 gaaatacccc tcagacatca cagcaatacg cgatgccatc gtcatcgcct cctcttggtc
     1861 atgcgttacc aacacacagg ttacaccaac ttcttcaata atattcacta actccagttg
     1921 cgtggtttcg cgcaattttt tatcaagcgc gcccagtggt tcatctaaga gcagcaattg
     1981 tgggcgttta gccaagctcc gcaccaaagc cacgcgctgt tgttgcccac cagaaagctg
     2041 atgtggcttg cgttgtgcaa atttacccaa ttgtgcaagg ttgagcattt tttctacgcg
     2101 ctctgcgatt tggtcttttg gcatgccatc gcgctttaag ccaaaggcta tgttttccca
     2161 tacactcaag tgtggaaaca acgcataaga ctgaaacatc atattgatag gacgctggta
     2221 aggaggcaaa ttggttatat cttgccctgc taagaaaatt ttgccttttg tgggcatttc
     2281 aaagcctgcc atcatgcgca acagtgttga ttttccgcag cctgaaccac ccagcaaagc
     2341 aaaaatttca cctttattca cggtcaatga gacatcatcc actgccttta ccgtgccatc
     2401 gtaaattttg gtgacatttt caacgcgcaa caatacttct ggcgctggct ggttgatttc
     2461 tttaggtgcg gttgccataa aaaaccctta gtaaatgatg atggtgtaaa cgagtaatca
     2521 gctaagtgtg ggcgtatgat acgcccacac ttgttagcta aacgccagat ttaaacttgg
     2581 tgtaaaagcg cgttaaagtc cgtttggctt cttggctttc aacacctggt ggcactagtt
     2641 ttgcaatatc ttcatcagat aagtagatgg acttattatt tttaatttct tcatcaacaa
     2701 actctgtagc tgctttattg gggtttgcat aggtcacctc attggtaata gatgccgcca
     2761 cttcagggcg catgacataa ttgatgaata aatgtgcgtt attaggatgt tttgcatccg
     2821 caggaatcgc catggtatcc ataaaaatca agccaccagt ttttggtaca agggctacaa
     2881 tattttgctc ggctttattt tctattgaac gcttgcgtgc caagttgata tcacctgccc
     2941 atccataggt tacacagacg ttaccacctg ccaaatcttc aatttggccg cctgaattaa
     3001 actttttaat gtgtgggcgc actttcatga gcatatcaaa tgctgcctga taatcatcat
     3061 ttgatgcggt gaataccggc ttgcctaaat aatgcaatgc cgcctgaaac acgtccgttg
     3121 gcgtatccag aaacgtgatg ccacatgatt ttagcttact ggtataagtt ggattaaaca
     3181 cgagatccca tgcattgtca ggcatcgggg tgtcgcccaa cgcggccttt actttatctt
     3241 cattaatacc cacggtgtta taactccaca tccagtccac caagtattgg ttaccggggt
     3301 caacaccttc catttgtttt aaaatgccct catctaaatt tttccagtta gtgagtttag
     3361 ttttatctaa cttttgaaac aaaccgccct caatttgctg tttggcccaa ttggatgaag
     3421 gtacaacgat gtcgtaacct gtgtttttgg caattaattt ggcatgtaga atttcattac
     3481 tgtcaaacac gtcgtaacgt actttgatgc cagtttcttt ttcaaaattt tctatggtat
     3541 cgggggcaat gtaatcagac cagttgtaaa tgttaagaat tttttcttcc tcgctggcaa
     3601 ccgttgattg cgcaactttt gcatcgctgg tggtttcctt attactgtca gtacatgata
     3661 ctgaaccaaa gccaacaatc gccaagccca ccatcgtaca taaaagacgt ttgttcaata
     3721 aaatttgcat cgaatgtctc ccaagaaaaa gtaaaaaatc tgaatcaatt ttaacagcca
     3781 aatttacttg ttgcactaac caaatgatta agttgcctta acttttagta atcaaaaata
     3841 caccggccgt attattaaaa gcaagttatg tgccctaccc tcaacttaaa cttaatataa
     3901 ttcattacat cacacccagc cttttcaaat cagccagcgt agcgtctaag cataaataaa
     3961 tgagccctat catttcatca atttgcgctt tggtcgtgac caatggtggt gcaataatca
     4021 tgcggtcgcc cacggcgcgc ataattaaac cgttagcaaa gcaatgcccg cgacacatca
     4081 tacctacttc taaactctca tcaaagtgaa catgctcttc tttgtttttc atgagaacaa
     4141 acccagcgac taagccaaat gtttcagcgc caccaaccaa tggatgttta gctaactcag
     4201 catatttttg agccagatac ggccctgttt catagcgtac ttggttaacc aaatcttcac
     4261 gctcgatgat ttcaagattc gccaatgcca cggcacatgc agttgggtgc ccagaatacg
     4321 tgtagccatg attaaactca ccaccctgct caatcagcac ttgtgcaacg cgtttaccga
     4381 ccaccacgcc ccctagcgga atatagcctg agctgacacc tttggcgaag gtcatcaaat
     4441 caggttttgc gcccatgcgt tgcgagccaa accacgcgcc taggcgacca aagccacaaa
     4501 tcacttcatc acaaatgagc aatatgccgt atttgtcaca aatccgctga atttctggcc
     4561 aataggtttg agggggaata atcacgccac ccgcgccctg cacaggttca ccaataaaag
     4621 ccgcaacctt atctgcacca acttctaaaa tcttttcttc cagccaagat gccgcgcgca
     4681 ggccaaaagc atcccgatct tcatcaggcg ccaagccata atgatagggt tgttcaatat
     4741 gctcaacacc tgaaatcacg cccccctgtg cgtgcatgcc gcccatgccc ccaagtgatg
     4801 aacccaccat ggttgagcca tgataagcat ttttacggct gataattact gtgcgttctg
     4861 gctgaccaag ggtagcccag taatggcgta ccatacgcac attggtgtca tttgactcag
     4921 accctgagct gccaaaaaat acatgcgaat aattatcacc tgcaagcgaa atgattttct
     4981 cggccagctt aaccgctggc acgttggtag tttgaaaaaa actgttataa aaaggtagct
     5041 cagtcatttg tttgcaagcc gcatccacca gctctttgcg accatagccc acattcacac
     5101 accacaaacc cgacattgca tcaaatattt tgtggccttc agaatcccaa atgtagacat
     5161 tgtcggcttt ggtgataata cgcgacccct ttgccgccag gcttttatgg tcggtaaacg
     5221 ggtgcatgta atgcgcggaa tcaatcgcct gaatttcttt agtatttgtc atgttattct
     5281 cgatgattga tttacttaaa cgtgcatgag cagatgttca cgctcccaag ggctaatcac
     5341 gcgcataaat tcatcatgtt ctgcatcttt aaccgctaaa taaacatcga cgaaatcttt
     5401 acctaacacc tcatgtaaag cggttgcaac ttctaattca cggatagctt ctgaaagccc
     5461 gcgcggtagt tggtagtctg aagcatatgc actacccgta gtttcagccg tgggttgtag
     5521 cttttcttta atgcccaaat aaccacaagc cagcgtgacc gccatcgcta aatatggatt
     5581 agcatcggca cccactacgc gattttctac gcgtcgccct gcaggttcag aaataggcac
     5641 acgaataccc acagtacgat tatcgtaacc ccattcaata ttgataggcg cggcaccgtt
     5701 acgcacaatg cggcgatacg agttcacata gggtgcaagc aacaccatgg cagcgggcaa
     5761 atatttttgt agccccgcaa tatagtgaaa gaaaagtggc gaagcagaac catctgcttt
     5821 gctgaaaata ttattgcctg atttgcaatc tatcacgctt tggtgaatgt gcatggccga
     5881 gcctggctca ttttgcatgg gttttgccat aaaggtcgcg tacatgccgt gacgcatggc
     5941 cacctctcgc aaggtgcgtt taaaaaagaa ggttttatcc gccaaaatca tcggctcatc
     6001 gtgcaaaaag ttaatttcca tctgccctgc gccaaattcg tgaatgagcg tgtctaactc
     6061 tagccccatc aaatcgcaat agtcgtaaat atcttcaaat aacgggtcaa attcattcac
     6121 tgcatcaata ctataaagct gacggctggt ttctttacgc ccacttcgtc ctaccggtgg
     6181 acttagtgct acgtccgggt cggtattgcg ttctagcaaa taaaactcta gctcaggggc
     6241 cacaatcggc gtaaaaccaa gttctttata taagttagtc acgcgacgca atacatttct
     6301 aggtgaaaaa ttaaccggcc ggccttggtg gtcaaagcaa tccatgataa cttgtgcggt
     6361 cgggtcttgc gcccaaggca ccacgcgcaa agtgctgggg tcaggaatca ataccatgtc
     6421 tttatcagta aacccaaaaa cacgatcgta accctcgttg tcttcaggtg attcgcctgt
     6481 taccgtcatg ccaaacacgg cttctggcaa gcgcatggcg cgttcggttg aaaacttttc
     6541 acgtggtaga attttcccac gcgccacccc cgttaaatcg ggcaccaaac attccacctc
     6601 ggtaatgttg ttatccgtca gccatttatc catatcaata tgattttttg cgcttgcttc
     6661 tgtcatgttt tgctcctgat tttactaatt tacgccgatt gatttaagat taaaacaatc
     6721 agagttaatc gggtggctcg tgacggcgca ttcttagcct gcaagcagca ccaaaagcgc
     6781 taaaaatggc taaatattgt ggattttgca tgacttgcca ttctggatgc cactgcacgc
     6841 ccagcgcaaa cgtttgcgca gcatccaccg aaaccgcctc ggccaagcca tctggcgcgg
     6901 ttgcttcaat ggttaaccct gcacccaaac ggtgaatccc ttgcccgtgt agtgagttca
     6961 cgaacatttc attcgactgt gtgatattgg ctagttttcc gccctcagtt aaacgtacgc
     7021 tgtggactgg tgcatattgc aacgctaaat cgacagtctt agcttctctg tggtcatgca
     7081 acccatcaac atcatgcaca gcctgatgca aactgccccc aaaagccaca tttaactctt
     7141 gaaagccacg acaaatcgca agtaacggca cgcctttttg tatggcggta cgaatcaaat
     7201 tcaacgtcag cgcgtctctt gcagggtcta gcggcaggga gggatcaagc acatcttgat
     7261 taaaatgcga agggtgcaca tttgaaactg acccggtgag caaaatacca tcggccattt
     7321 caagcagcgc ttctaaatca agcgcatcgg ctacggcagg aatcagtaaa ggtgtggctt
     7381 ccgccgcgtc agccacggcc aaaatatact tctgccccac cgcatgaaaa agatggtcgc
     7441 ccagcggttt tacatccgcc ggcaagagaa ctactggttt tttttgcttg tttttcatag
     7501 taaaacgtat ttactgtcac tgttataaaa accacaacag cacaataaac tgtttctata
     7561 atttcaagtt tctgtaattg tatgtttgaa gtaatccacc actttgaaca catactacgc
     7621 cttaggcaac gcgcctagca agtagtttat gtaaattatt gtggtcaatt ttaaaaagcg
     7681 tcttcgttaa aataccgatt gcaaacggct ttaaatttat taaaaattaa cgtaattttg
     7741 catgtaattt taaccaaacc tttttcgatg ccctggaagc cttataaata aaggctcgca
     7801 gagcataatt ttattctcgg acaaaattgg tgatattttt gactttaata tatcaacact
     7861 aaactttgac gaacagtcag cacaagaccc gaacgggtga ttgctacatc cacgcacaag
     7921 cctatagttt tacttacaat aattaacaat tctgtgtact taagcgaact gaaagttagg
     7981 ttaaaagtaa tgaacaaaat tagtcaattg acctcaatgt tatttgtagc ctctggcttg
     8041 ttctttgggt taaacccttc agcaatggca gcacctgtgg tgtggacatt taatttaccc
     8101 tctacggcag tcgcttcaca aagcccgccc tatgcatcag ttgcgacgct gtcactgacg
     8161 caaattggat cagacgtgca attttttctt gatccgaatg aattgaatgc cggtgcaagt
     8221 ccagcgacaa gcacctttgt tcattctatc gatttcgttt attcaggcgc agtattaact
     8281 gcggcaaatt ttgcctataa cgcaggtgct tcggttgata ctttttctta cgaggtaaat
     8341 caaaacaact tggacgctgg ctatactacg caagaccagc acgtaaaagt tgattttttc
     8401 tcaaaaaaca gtgatggcgg tgtccgtttt gattttactg aaacaagtac ctggacaata
     8461 aaagatgttt cattaacaaa cttcacaaca acttatgcaa cgagtggtcc aaaacccagc
     8521 ccaatttatg gggttatatc tttaggtggc tttagtggcg gcacatcgaa ctgggttgca
     8581 gcagtaccag aaccagaaac ctatggcatg atgctcgtgg ga
//
LOCUS       DAOQSI010000084         8488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_7879_length_8488_cov_4.360014, whole genome shotgun sequence.
ACCESSION   DAOQSI010000084 DAOQSI010000000
VERSION     DAOQSI010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 8488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8488
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7879_length_8488_cov_4.360014"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..613
                     /locus_tag="PKL42_06515"
     CDS             92..613
                     /locus_tag="PKL42_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNU66768.1"
                     /translation="MSSQSLFSTITAHYTKATGLLESLADYFPNLLIRIILAWEFGEA
                     GFEKLRGTNWFTEISFPFPFSLLPAEISWNMAMFFEIVGAFALLFGFATRFFSISLLI
                     LTVVAIAAVHWPEQWGTISELLKGYRIIDENGDGFGNYKLPVIFIIMFLPLIFSGAGK
                     LSLDYLVKKYLAK"
     gene            complement(832..1275)
                     /locus_tag="PKL42_06520"
     CDS             complement(832..1275)
                     /locus_tag="PKL42_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66769.1"
                     /translation="MHLNLRKISLNISLVTLTLLALSLSAIQVNAAEKEASKTTSKAK
                     VNKASKSTSYTEDQFLNAFSGKMKSVVLAQLGNPAKKEQSVKPAGASGYLGRVGADEK
                     NSKRVNVEMWYYKDLVNYDPKNKYKETEITFVNDRVMNIAFFNNR"
     gene            complement(1384..2148)
                     /locus_tag="PKL42_06525"
     CDS             complement(1384..2148)
                     /locus_tag="PKL42_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNU66770.1"
                     /translation="MLVTMLGVGSSAGTPMIGCKCKTCTSTDARNQRTRCASLITLDN
                     GRNILIDTGPDLRQQALREGITHIDAVLYTHTHADHLHGIDDLRSFCQIQRSQIPLYA
                     SHTAVKHITDKFGYTLREPSDYWDLPVLKIQAIEAAFELFGQKIIPIPIKHGNSDIFG
                     YRIGNLAYLTDVSAITEASLQLLDNLEVLLLDCLRYTPHFTHINVEQSLNYASLINAK
                     STYLIHMTHELEYTELTAKLPSNVYVGYDGLKINYQ"
     gene            complement(2182..2952)
                     /locus_tag="PKL42_06530"
     CDS             complement(2182..2952)
                     /locus_tag="PKL42_06530"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNU66771.1"
                     /translation="MLVDSHCHLNFPELLENLTVIKQSMQDNQVGHALCISVTLPDFP
                     QVLALAETNDNFYASVGVHPDYENIQEPDVDELIALANHPKVIAIGETGLDYYRLTGD
                     LEWQRERFRTHIRAAIACGKPLIIHTRNAAEDTLRIMHEENAQQIGGVMHCFTESLEV
                     AKQAIDLGFYISFSGIVTFKNALSIKEVAKNIPLDKMLIETDSPYLAPMPHRGKTNQP
                     SYVKFVAQEIATLRGISFDEVAAATTQNFFKLFKHASV"
     gene            complement(2982..3359)
                     /locus_tag="PKL42_06535"
     CDS             complement(2982..3359)
                     /locus_tag="PKL42_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNU66772.1"
                     /translation="MAEDTQDDFKNTTTPKPGVLSLAIKEKAALYAAYMPFLKGGGLF
                     IPTSKPFNIGEEIFMLLSLVDDPMKLRVMGHVVWVTPTSQAGKPQGVGVQFSEKDGGL
                     EAKNKIEALLAGALKSSRPTHTM"
     gene            complement(3393..4406)
                     /gene="holB"
                     /locus_tag="PKL42_06540"
     CDS             complement(3393..4406)
                     /gene="holB"
                     /locus_tag="PKL42_06540"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HNU66773.1"
                     /translation="MAKDFHPPYPWQTGLWQQLRHDRQQLGHALLLYGRAGIGKYVFA
                     RYLSQALLCKQPHSGHPCGECASCHWFNEESHPDFRLLSPEQAAESDDESAPSKKTKK
                     KTQISVSQIRDLNEFLSLSSHRYDGARIVLIQPAEALNAASANALLKMLEEPASGVIF
                     ILVANQLQRLLPTIISRCQKVSMPAPSESQALAWLSEQGVQNAQQQLAYASGSPIQVV
                     NEQAQSTTLADIWQQLALGSKLQPHLAASMLIAHSAETGIIALQKWLCDLLTMRLTKQ
                     VHYHVKQSGALQGLSDKVNLSQLMQLQKKVDELRKLALHPLNHELQLEALLVEYTRVF
                     VNK"
     gene            complement(4409..5014)
                     /gene="tmk"
                     /locus_tag="PKL42_06545"
     CDS             complement(4409..5014)
                     /gene="tmk"
                     /locus_tag="PKL42_06545"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNU66774.1"
                     /translation="MTGKLITLEGMDGAGKSTHIANIISALVARGHEVVSTREPGGTA
                     LGERLRELLLHESMHAETETLLMFAARREHIAQVISPALARGAYVLSDRFTDATYAYQ
                     AGAKGVDINKIRTLEQWVQGALQPDITLLFDVPVEVSMQRLVQAREPDKFERENAAFF
                     TKIRQMYLQRAAENPARFHVIDANQPLEAVRQSVLALVSTL"
     gene            complement(5032..6027)
                     /gene="mltG"
                     /locus_tag="PKL42_06550"
     CDS             complement(5032..6027)
                     /gene="mltG"
                     /locus_tag="PKL42_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNU66775.1"
                     /translation="MMKSIKTWLLLACVVLIGIGAWLAYYAVTPLKLAPSSQEIVIQP
                     KSGLKSIANQLVAQGVIQEPWRFIVLAKLLNKESTLQAGNYTLNKNVSPYQLLLSLNN
                     GKATQGSITFIEGRTFAQMREKMARNDALKQTVSTLSETEILKLVGSNRSKAEGLFFP
                     DTFYFDRNTADTVILQRSYDAMQSKLEAAWQKRAPNLPYKNSYEALIMASIVEKETGK
                     ASERAMIAGVFINRLRIGMRLQTDPTVIYGMGDEYKGNIRKKDLQKDTPYNTYTRDGL
                     PPTPIAMPGLAAIEAALHPEATKALYFVGKGDGSHAFSSSLQEHNRAVVKYQLKK"
     gene            complement(6041..6886)
                     /gene="pabC"
                     /locus_tag="PKL42_06555"
     CDS             complement(6041..6886)
                     /gene="pabC"
                     /locus_tag="PKL42_06555"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HNU66776.1"
                     /translation="MSAPTYIINGSFNHHITPLDRGFSYGDGVFRTMKMRGGLPLNWP
                     LHYQKLVADCAVIGIVCPSAELLMRDFEQLFTLQDADKQAENVAKIVITRGEGERGYQ
                     PPAVTSPTRVVIKTSLPQYLATYDEAGVTLTLCQTRLSAQGKLAGVKHLNRLENVLAR
                     MEWRDEAIFDGLLLDQHHHVIECTMSNVFMRVGNTLFTPDLSQCGVAGVTRQCVLNVA
                     HVLNLKPEITSFTFEDVLKADEVMICNSLFGCYQVTRMLDQTWPMQSMARSFKQLLAK
                     QSVNA"
     gene            complement(7190..8422)
                     /gene="fabF"
                     /locus_tag="PKL42_06560"
     CDS             complement(7190..8422)
                     /gene="fabF"
                     /locus_tag="PKL42_06560"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNU66777.1"
                     /translation="MSKRRVVVTGLGVISPVGNGVQTAWKNLIAGKSGITNITKFDTS
                     AFASTVAGEVKDFNVEDYLSAKDARRMDTFIQYGLAAGIEAFKDSGIEVTEENAERIG
                     VSIGSGIGGMQFIEDTDVLYHESGPRKISPFFIPGTIINMISGNLSIMFGLKGPNVAI
                     VTACTTGTHSIGDASRMIEYGDADVMIAGGAEAAITQLSVGGFAAARALSTRNDDPAT
                     ASRPWDKDRDGFVIGEGAGVMVLEEYEHAKKRGAKIYAELSGYGMSADAYHMTAPNMD
                     GPRRSMRNAMQNAGINPDAVQFVNAHGTSTPLGDANETNAIKAAFGEHAYNLVVNSTK
                     SMTGHLLGGAGGLESVFTVLSIHHQISPPTINIFNQDPECDLDFCANTARDLKIEYAL
                     KNNFGFGGTNGSLVFKKV"
BASE COUNT         2303 a         1887 c         1788 g         2510 t
ORIGIN      
        1 taaatatatt gataaaattg cttgtaaggc ttgtttaaac gaagcgacta agggctaggg
       61 ttcagaacaa tcattttaaa gggagatttt catgtctagc caaagcttat tttcaaccat
      121 tactgcacat tacaccaaag cgacaggctt attagaaagc ctcgccgatt actttccaaa
      181 tcttttgata cgcatcattc tcgcatggga gttcggtgaa gcaggttttg aaaaacttcg
      241 cggcacaaac tggtttactg aaatcagctt tccattcccg ttcagccttt taccagcaga
      301 aatcagctgg aatatggcga tgttctttga aatagtcggt gcatttgcac ttttatttgg
      361 cttcgctaca cggtttttct caatctcatt gctcatatta acggttgttg ccattgccgc
      421 ggtgcactgg ccagagcaat ggggcacaat ttccgaactg ctaaaaggtt accgaattat
      481 cgatgaaaat ggtgacggct ttggtaacta taaattgcca gtgattttta tcatcatgtt
      541 tttaccgcta atttttagcg gcgctggtaa attaagcctt gattatttag taaaaaaata
      601 tttggcaaaa tgaatgaaat gcgtttgaaa ctttgtaacc aatatatttc ttacgcattt
      661 taaatattaa gtcagcaggt tagttcattt tcatgtcaca tacgcttact atcaaagcac
      721 aatcttaatt ttacataata tacattatac gaagtatgta atatgtaaag aaggccaagc
      781 tttgcgcttg gccatctaat tttaatgctg ctataactac tgctgaatca attatctatt
      841 attaaaaaaa gcaatgttca tcactcggtc attcacgaat gtaatttcag tttctttgta
      901 tttatttttt gggtcgtaat tcactaaatc tttgtagtac cacatttcaa catttacacg
      961 tttagagttt ttttcatcag caccaacacg acccaaatac ccactagcac ctgccggttt
     1021 tactgactgt tctttttttg cgggattgcc taattgagcc aataccacac ttttcatttt
     1081 tccgctgaaa gcatttaaaa attgatcttc agtataactt gttgatttgc ttgctttatt
     1141 taccttcgct ttgcttgtcg ttttggaggc ttctttttct gcagcattta cttgtatagc
     1201 acttagtgac agagccaata aagtcagtgt gaccaagcta atgttgagtg aaatttttct
     1261 taaattgagg tgcatgtctt aaatctccaa atgtaatttg ttacctttta cattacctag
     1321 taactttatc gcgccattat agcgcacctg ataactcaac gcaccaaacc caattaagct
     1381 gacctactga taattgattt ttaggccatc atagcccaca tagacattac tcggcaattt
     1441 tgcagtgagt tctgtgtatt ccagttcatg tgtcatgtga atgagataag ttgatttagc
     1501 gttgatgaga ctagcgtagt tgagactttg ttccacatta atatgggtaa aatgtggtgt
     1561 atagcgcaag caatctaaaa gtaacacttc taaattatcg agtaattgta aagaggcctc
     1621 agtaatcgct gaaacatcag taagatacgc cagattgcca attcgatagc caaaaatatc
     1681 actattgccg tgttttatag gaatgggaat aattttctga ccaaataatt caaaggcagc
     1741 ctctatggct tgaattttaa gcacaggtaa atcccagtag tcacttggct cccgcaaggt
     1801 gtaaccaaat ttatcggtaa tatgttttac cgctgtgtgg cttgcataaa gcggtatttg
     1861 gctgcgttgg atttgacaaa agcttcttaa gtcatcaatg ccatgtaaat ggtctgcgtg
     1921 cgtgtgtgtg tagagcacag catcaatatg ggtaatccct tctctcaatg cttgttgcct
     1981 taaatcaggc ccagtatcaa tcagaatatt tctgccattg tcgagtgtaa ttaggctagc
     2041 gcagcgcgtg cgttgattgc gtgcgtcagt tgaggtgcag gttttgcatt tacacccgat
     2101 catgggcgtg ccagcgcttg aaccaacacc cagcatggtc actaacaagc gcttatgcct
     2161 tttctcgtgc agtaattgtg attacacaga tgcatgttta aataacttaa aaaagttttg
     2221 cgtggtagct gctgcaactt catcaaaact aattccgcga agcgtcgcaa tttcttgcgc
     2281 gacaaacttc acataacttg gctggttggt tttaccgcga tgtggcattg gcgctaaata
     2341 aggcgaatct gtttctatta acattttatc tagcggaata tttttagcga cttctttgat
     2401 agacaaggcg tttttaaaag tcacaatgcc tgaaaacgag atataaaaac ccaaatcaat
     2461 ggcttgtttt gccacttcca aactctcagt aaaacagtgc ataacgccac caatttgctg
     2521 ggcattttct tcatgcatga tgcgcagggt gtcttctgca gcatttcttg tatgaatgat
     2581 gagcggcttg ccgcaggcga ttgcggcgcg aatatgcgtg cgaaagcgtt cacgctgcca
     2641 ctctaaatca cctgtgagcc tatagtaatc gaggccagtt tcaccaattg caatcacttt
     2701 tgggtgattg gcaagtgcaa tcagttcatc aacatcgggt tcttggatat tctcataatc
     2761 tgggtgcacg ccgactgaag catagaaatt atcattggtt tcagccaagg ctaatacttg
     2821 aggaaaatca ggcaatgtga cagaaataca taaagcatgc cctacttggt tgtcttgcat
     2881 tgattgcttg atgacggtta aattttcgag taattcagga aaattcaggt gacagtgtga
     2941 atctacaagc atggataaga tggataattt ggccgttaag tttacatggt atgcgtgggg
     3001 cgagaagatt ttaatgcccc tgccaaaagc gcttcaattt tgttttttgc ttcaagacca
     3061 ccatcttttt cactaaattg cacccctacc ccttgtggct ttcctgcttg agaggttggt
     3121 gttacccaaa ccacgtgccc catcacgcgt agtttcattg ggtcgtccac taaacttagt
     3181 agcataaata tttcttcgcc aatattaaat ggcttgctgg ttggtataaa tagaccgcca
     3241 ccttttaaaa aaggcatata ggccgcatat agcgcggctt tctctttaat cgcgagggat
     3301 agcacaccag gctttggggt ggtggtattt ttaaaatcgt cttgtgtatc ttcagccata
     3361 gtttttctct attgatcacg tttcagcatt atctacttat tcacgaaaac tctcgtgtat
     3421 tctacaagca acgcttctaa ttgcaactca tgattaagcg gatgcaaggc tagctttcgc
     3481 agttcatcca cttttttctg taactgcatt aactggctta agtttacctt gtccgataag
     3541 ccttgcaaag cgccagattg cttaacgtgg taatgcactt gttttgtcag gcgcatggtt
     3601 aacaaatcac atagccactt ttgcagtgca ataatgcctg tttcagcaga atgtgcgatc
     3661 aacattgagg ccgcaagatg tggttgcaat ttgctgccta aagcaagctg ctgccagatg
     3721 tcagcaagtg tggttgactg cgcctgctcg ttgaccacct gaatgggaga acccgaagca
     3781 taggctaatt gttgttgtgc attttgcacg ccctgctcac ttaaccatgc caatgcttga
     3841 gattcactgg gtgctggcat gcttactttt tgacaacggc ttatgatggt aggcagtaaa
     3901 cgttgcagtt ggttggcaac taaaataaaa ataacccctg atgcaggttc ctcaagcatt
     3961 tttaataacg cattggcaga agcggcattg agtgcctcgg ccggctgaat taacacaata
     4021 cgtgccccgt catagcggtg actactcaag cttaaaaact cgttcaagtc acgaatttgc
     4081 gacactgaaa tttgtgtttt ctttttagtt tttttgcttg gtgccgattc atcatcagat
     4141 tccgcagctt gctcgggcga cagcaagcga aaatcagggt ggctttcttc gttaaaccaa
     4201 tggcaactgg cgcattcgcc acaagggtgg cctgaatgcg gttgcttgca tagtagcgcc
     4261 tgacttaaat aacgcgcaaa gacgtatttt ccaatacctg cgcggccata caacaacagg
     4321 gcatgcccga gctgctggcg gtcatgcctt agctgttgcc aaaggccggt ctgccatgga
     4381 tacggtggat gaaagtcttt tgccatcgtt acagcgtgga aaccagcgcc aacacagatt
     4441 gtctgacagc ttcaagcggc tgatttgcgt ctatcacatg gaatctagca gggttttcag
     4501 ccgcgcgctg caaatacatt tggcgaattt tagtaaaaaa tgcagcattt tctcgctcaa
     4561 atttatctgg ttcacgcgct tgtactagcc gctgcatact cacttccaca ggcacatcaa
     4621 acaataaggt gatatctggc tgcaacgcgc cttgcaccca ttgttcaagc gtacggattt
     4681 tattgatatc aacaccctta gccccagcct gataggcgta tgttgcatca gtaaaacggt
     4741 ctgaaagcac gtaagcccct cttgccagcg ccggactaat cacttgcgcg atatgctcac
     4801 gccttgccgc aaacatcagt aaggtttcag tttcagcatg cattgattca tgtagtaata
     4861 actcacgcaa ccgttcaccc aatgccgtgc cgcctggttc gcgcgttgaa acaacttcat
     4921 gaccgcgcgc tactaaagct gaaatgatat tggcaatgtg ggtgctttta ccagcgccgt
     4981 ccatgccttc caaggtgatc aatttacctg tcattagtgt acttttttga actatttttt
     5041 gagttgatat ttaacgaccg cacgattatg ttcctgcaaa ctacttgaaa aggcgtggct
     5101 tccatcgcct ttgccaacaa aatagagtgc tttagtcgct tctggatgta acgcggcttc
     5161 aatggcagca agccctggca tggcaatggg tgtcggtggc aaaccatccc gcgtataagt
     5221 gttgtaaggc gtatcttttt gcaaatcttt ttttctgata ttgcctttgt attcatctcc
     5281 catgccgtaa atcacggtag ggtcagtttg caggcgcata ccaatgcgta aacggttaat
     5341 gaaaacgcca gcaatcatgg cacgctcgct ggctttaccg gtttcttttt caacaatgga
     5401 agccataata agtgcctcat agctgttttt atatggcagg tttggcgcgc gcttttgcca
     5461 agcggcttca agtttgctct gcatggcatc gtaactgcgc tgcaagatga cggtatctgc
     5521 tgtgttgcgg tcaaagtaaa acgtatcagg aaaaaacaag ccttccgctt tactgcgatt
     5581 tgaaccgaca agctttaaaa tttcagtttc agaaagcgta ctcacggttt gcttaagcgc
     5641 atcattgcgc gccatttttt cacgcatttg tgcaaacgta cggccttcta taaaggtaat
     5701 gctgccctgc gttgccttgc cattattgag tgataacagc agttgatagg gtgaaacatt
     5761 tttattcagc gtgtaattgc ccgcctgcaa tgttgattct ttattcaaca gctttgccag
     5821 aacaataaaa cgccacggtt cttgaattac gccttgcgct accagttgat tggcgatact
     5881 ttttaaacca cttttaggtt gaatgacaat ttcttgactg ctcggagcga gctttaacgg
     5941 cgtgactgcg taataggcta accatgcgcc aatgccaata agcacaacgc aggcaagcaa
     6001 aagccatgtt tttattgatt tcatcatgac accgttttca tcaagcgtta accgattgtt
     6061 ttgcaagtag ttgcttaaac gacctcgcca tactttgcat aggccaggtt tggtccaaca
     6121 ttcgcgtgac ttgataacag ccaaacaagc tattacaaat cattacttca tcggctttta
     6181 aaacatcctc aaaagtaaac gaggtaattt caggttttaa attaagtaca tgcgctacat
     6241 ttaacacaca ttgccgcgtc acaccggcaa caccgcactg gcttaaatct ggtgtaaaaa
     6301 gagtattgcc aacgcgcata aatacattac tcatcgtaca ttcaataaca tggtgatgtt
     6361 ggtcaagcaa taaaccgtca aaaatcgcct cgtcacgcca ttccatgcgc gctaaaacat
     6421 tttctaagcg atttaagtgt ttaaccccag ccagtttacc ttgtgcgctc aaacgtgttt
     6481 ggcaaagcgt tagtgttacg ccggcttcgt catacgtcgc taaatattgc ggcaagctgg
     6541 ttttaatgac aacgcgtgtt ggtgaagtga ctgccggtgg ctgatagccg cgctccccct
     6601 ccccgcgtgt gatgacgatt ttggcaacgt tctctgcctg tttgtcagcg tcttgtaacg
     6661 tgaatagttg ctcaaaatca cgcattaaca actcagcact ggggcacaca atgccaatga
     6721 cggcacaatc tgccactaat ttttgataat gcagcggcca atttagcggc aaaccaccgc
     6781 gcattttcat ggtgcggaac acgccatcgc cataactaaa accacgatct aacggcgtga
     6841 tatggtgatt aaaactgcca ttaataatgt aagttggtgc agacatatgc caagcattat
     6901 aaacgatact ggttcacgat tggggcacag cttgatgaag aattactcag aatctttgct
     6961 taaaaagtga gatttcttgt ggttgtaaga cgctcaaaat acacatgata aaatgtgctt
     7021 actcataaca aaaaacgacc gagaaaaatt ttatgccctg cgagccttta tttataaggc
     7081 tcgcagggca tcgaaaaagg ttgcaggtaa atatgtgctt aaaaagacat tttttagcac
     7141 aatttaatgt aagaaaagtg acactttttt taaagactaa actgccacgc tatacttttt
     7201 tgaaaactag gctgccattc gtgccgccaa agccaaaatt gtttttcagc gcgtattcaa
     7261 tttttaaatc acgtgcggta ttggcacaaa aatccaaatc acattctggg tcttgattga
     7321 aaatgttaat ggtgggcggt gaaatttgat ggtgaatcga cagcacggta aaaactgatt
     7381 ccaacccacc tgccccgccc aataaatgtc cagtcatcga tttggtagag ttcaccacca
     7441 aattgtaggc atgttcacca aatgcggctt taatggcatt ggtttcgtta gcgtcaccca
     7501 acggtgttga agtgccgtgc gcgttcacaa attgtacggc atcagggtta atgccagcgt
     7561 tttgcatcgc gttacgcata gacctgcgcg ggccgtccat atttggggcg gtcatgtggt
     7621 aggcgtcggc gctcatgcca taacctgaaa gctcggcata aattttggcg ccacgttttt
     7681 ttgcatgctc atattcttca agcaccatca caccagcgcc ttcgccaatc acaaaaccat
     7741 cacggtcttt gtcccatgga cggctggcgg ttgctggatc atcgttgcgt gtggataacg
     7801 cacgtgcagc cgcaaagcca cccacgctca attgcgtaat agcggcttcg gcgccgcctg
     7861 caatcattac atcagcgtcg ccgtattcaa tcatgcgtga agcatcacca attgagtgtg
     7921 tgcctgtggt acaggctgtc acaatggcca cattggggcc tttcagccca aacataatgc
     7981 ttaaattacc ggaaatcata ttgataatgg tgccgggaat aaaaaacggt gaaattttac
     8041 gcggaccaga ctcatgataa agcacgtcgg tatcttcaat aaactgcatg ccgccaatac
     8101 cagaaccaat ggacacgcca atacgttcag cattttcttc ggtgacttca atgccagaat
     8161 ctttaaatgc ttcaatgcct gctgccaagc catattgaat aaacgtatcc atgcgtctag
     8221 catctttagc acttagataa tcttcaacat taaagtcttt cacttcgcca gccacagtgg
     8281 acgcaaaagc gctcgtatca aatttggtga tgttggtaat acctgattta ccagcaatta
     8341 agtttttcca agccgtttgg acgccattgc caacgggaga aataacacca agccctgtaa
     8401 ccactactct gcgtttagac atgagaaacc tcgaatgttg atgatatgtg tgaatgcaaa
     8461 taaaaaggca ataaaccccg ataggagt
//
LOCUS       DAOQSI010000085         8286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_8167_length_8286_cov_6.931479, whole genome shotgun sequence.
ACCESSION   DAOQSI010000085 DAOQSI010000000
VERSION     DAOQSI010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 8286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8286
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8167_length_8286_cov_6.931479"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..672
                     /gene="ybgC"
                     /locus_tag="PKL42_06565"
     CDS             238..672
                     /gene="ybgC"
                     /locus_tag="PKL42_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HNU66778.1"
                     /translation="MQNTGDFIWPIRVYYEDTDAGGVVYHSNYLNFMERARTEWLRAL
                     GFEQTHLKDDLGVMIVIHSLNIAFKSPARFNDLLQVHCQFANAGRSSMDIAQTIFRDG
                     VMLAEANVKAVFVDAASFRPVAIPQAIKDAAMGLTEAALTKT"
     gene            737..1411
                     /gene="tolQ"
                     /locus_tag="PKL42_06570"
     CDS             737..1411
                     /gene="tolQ"
                     /locus_tag="PKL42_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HNU66779.1"
                     /translation="MDVANDMSIFSLVAGASLPVQLVMIILLLTSLFSWWYIFIKVAT
                     IKRAETNAEDFEKAFWTGGDLNKLYEGLSVGRRKPQGMASIFEAGFKEYARHKQQGRT
                     EVSDIMEGTRRAMRAAYNREMDDLDAHLPFLASVGSVSPYIGLFGTVWGIMNSFRGLS
                     NVAQATLSQVAPGIAEALIATAIGLFAAIPAVIAYNRFASSVDRLSVRYESFMEEFTN
                     ILQRKS"
     gene            1415..1816
                     /locus_tag="PKL42_06575"
     CDS             1415..1816
                     /locus_tag="PKL42_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNU66780.1"
                     /translation="MARTRKRRMMNQINVVPYIDVTLVLLVIFMVTAPMTNPGVVDLP
                     KVGQGALVQKQVPPIVVTIKSNDKIEFEGAAVSSDVLLLKVKQALDANPQRAVVIAAD
                     KNVKYDDVTRVVGLFKQNKDIKLGLLLAPAP"
     gene            1828..2667
                     /locus_tag="PKL42_06580"
     CDS             1828..2667
                     /locus_tag="PKL42_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNU66781.1"
                     /translation="MDFNTMRGPSQTTMLKSYEKEISWKAGAYAIAVHLALLGGLLIS
                     FNWKAAHPMLNVAEVELWESIPSSAPPPPLIQPKVEPKIEPAPVVKDVPEPKPEAKVE
                     EPKVDIELEKKKELEQKKLEEKAAKLRKERLAAIQADTLKDEKRDSEKQLNDQKDKDA
                     IKKIQQDIDADGNQQALAAKASANAGVIDEYKRKIQDKIKGNVNNELCGDGKPVLKFK
                     IDLLPSGELSGSPKLTKTSGIEACDDAVERAIMASQPLPLPEDVALKSQFRNLNLTFT
                     PNN"
     gene            2807..4090
                     /gene="tolB"
                     /locus_tag="PKL42_06585"
     CDS             2807..4090
                     /gene="tolB"
                     /locus_tag="PKL42_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HNU66782.1"
                     /translation="MKIFRFLAFFTSFFASTFLFTPVANAALEIEISGGSAQQIPIAI
                     VPFGQGEHAATMSGIIAADLKRSGLFRVLETGGVASRPTDITQIKFAEWSALQAQAMS
                     VGTIEPQADGKLKVSFQLADVLKQTQLTSGQYNISPSQLRITAHKIADAIYQKLTGES
                     GIFASHIAYINKSKGRYTLQVADADGANPRTMLSSKEPIISPAWAPDGNRIAYVSFEK
                     KKPIIYVQTLGGQRTTVASFKGNNSAPAWSPDGNKLAIVLTHGANSQLYTINADGSGL
                     KQVTKSSAIDTEPTWSTDGQWIYFTSDRGGRPQIYKVSSQGGDPKRVSFEGAYNVSPR
                     FSPDGKSLAMIRNDGGRFRVALQDLNSGQVQLLSDGGQDESPSFAPNGRVILYATRNG
                     GRGALAAVSADGRVKQRLNESGGDIREPAWGPLIK"
     gene            4184..4756
                     /gene="pal"
                     /locus_tag="PKL42_06590"
     CDS             4184..4756
                     /gene="pal"
                     /locus_tag="PKL42_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HNU66783.1"
                     /translation="MNNLTFNKKLLSTLMLALVLTACKSTPMTKPSIEDKSVGQNTTT
                     TTPAAGADDGSTVKEVSLDDANANDAGNPLKDPNNILSKRNIYFDFDSDAVKAEYRPL
                     VEAHAKYLLSHKDARVIVQGNADERGTREYNLALGQRRSVAVKKALNVLGVQDEQIET
                     VSYGEEKSTGCADEACFSKDRRADIVYQGE"
     gene            4830..5687
                     /gene="ybgF"
                     /locus_tag="PKL42_06595"
     CDS             4830..5687
                     /gene="ybgF"
                     /locus_tag="PKL42_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HNU66784.1"
                     /translation="MMKKLNLVSLFLLVQLFAINSHAALFDDKEARKKILEIEATMTS
                     QHQATQNELANLKKSQQALEQRVIAIEAITKGQVLGDMQNHIDTLNQEIARLKGELEE
                     ANHHIEATQQRQKDLYGDTDARLRKLEGGDAAAATTTAPDATAAAASAATATPNKNTQ
                     EYQLLELANGLSREAKHKDAYNAYDKFLKDYPDSALAPEAKYGLGYTQFALKNYKSAI
                     ITQQKVIDLHPDSPKVPDAMLNKANSQIQLGQVANAKKTLRDLIAKFPESEVTPTAQR
                     RLKALEAIK"
     gene            5927..6565
                     /gene="queE"
                     /locus_tag="PKL42_06600"
     CDS             5927..6565
                     /gene="queE"
                     /locus_tag="PKL42_06600"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HNU66785.1"
                     /translation="MALKIHEIFYSLQGESTRVGLPTVFVRLTGCPMRCVYCDTAYAF
                     SGGSNMETADILAKIAEYGTKYVTVTGGEPLAQKECMVLLKALCDAGYNVSLETGGAV
                     DIGPVDSRVSVILDIKTPDSGELKNYLWENLTHLKKTDEVKFVLCSRTDYDWAKQILV
                     QYHLVEKCSVLFSPVYSQINPTDLANWVLADQLPVRMQLQLHKILWGEVPGK"
     gene            6562..7242
                     /gene="queC"
                     /locus_tag="PKL42_06605"
     CDS             6562..7242
                     /gene="queC"
                     /locus_tag="PKL42_06605"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNU66786.1"
                     /translation="MTKRAVVLLSGGLDSATVLAYARSLGFECYCLSLDYHQRHIAEL
                     HAAKNIAKLMNAKAHKTAQLDLSLFGGSALTDDAIAVPESATTGIPITYVPARNTIML
                     SLALAWAEVLEAQDIFIGVNALDYSGYPDCRGEYVKAFQSMANLATKSAVEGKTITIH
                     APLIDMTKAQIVQLGTKLGVDYAMTVSCYQADEHGEACGLCDSCRLRRDGFLAAGIDD
                     PTRYKALP"
     gene            7372..7866
                     /gene="rpsP"
                     /locus_tag="PKL42_06610"
     CDS             7372..7866
                     /gene="rpsP"
                     /locus_tag="PKL42_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNU66787.1"
                     /translation="MVIIRLARGGAKKTPFYNVVVADSRNRRDGRFIERVGFYNPSAK
                     ANAESLRIDLARVTHWQNNGAQLSETVARLVKFHAKGPEHAIAAKVKDAAKADAAKAK
                     IAAAEAAKVKAAADAAKAEADAKAAEEAAAAKEAEAAAKAAEAEAAAAPAEEAPAEEA
                     PAAE"
     gene            7937..>8286
                     /gene="rimM"
                     /locus_tag="PKL42_06615"
     CDS             7937..>8286
                     /gene="rimM"
                     /locus_tag="PKL42_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883636.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNU66788.1"
                     /translation="MGRIVAPYGVFGWLKVIPDTEALDGLLDYDSWWLGKGNDWRELK
                     VVEAKIHNDVIVVKLEGINDRDAAFACKGKQVAVPRDALPEPEENEYYWSDLIGLRVK
                     NKQDIDFGTIVDVF"
BASE COUNT         2381 a         1736 c         2008 g         2161 t
ORIGIN      
        1 atgcgcgtgg ttttttcttg gtttaaattg ggcttaattt gcaccgagaa aaatttatgg
       61 ctctgtaagc ctttatttat aaggctcaca gagcatcgaa aaaggttgtg ctgtaaattg
      121 cctaaaaatt aagcaatttc attttgctca aagcatctgc tattttgtca tttttgaaac
      181 aattacttaa caatttgaca cgccaccata ttaagttggc tgtgttaaaa tttgcccatg
      241 caaaacacgg gcgatttcat ctggccaatt cgcgtctatt acgaagacac agacgcgggt
      301 ggcgtagttt atcattcaaa ttatcttaat tttatggagc gcgcccgcac cgaatggctg
      361 cgtgctttgg ggtttgagca aacgcattta aaagacgatt taggtgtgat gattgtcatc
      421 catagcttga atattgcttt taagagccca gcgcgtttta atgacttatt gcaagtgcat
      481 tgccagtttg caaacgcagg gcgcagcagt atggacatcg cccaaacaat ctttcgtgat
      541 ggcgtgatgc tggccgaggc aaacgttaaa gcggtgtttg tggatgctgc gagctttcgg
      601 ccagtggcta ttcctcaagc aattaaagac gctgccatgg gattaaccga agcagcgctt
      661 acaaaaacat gatgacggtg caattttccc caacttttta tactattaaa actaaactat
      721 tggagctttt atgagtgtgg acgtagcgaa tgatatgtcg attttttcgc tggtggcggg
      781 tgcgagctta cccgtgcaac tggtgatgat aattttactc ctcacatctt tattttcatg
      841 gtggtatatt tttattaaag tggccaccat caagcgcgct gaaaccaatg ccgaagactt
      901 tgaaaaagcc ttttggaccg gtggcgactt aaataaactt tatgaaggtt taagtgtagg
      961 ccgccgaaaa ccacaaggca tggcgagcat ttttgaagcg ggttttaaag aatatgcgcg
     1021 ccacaagcaa caaggccgca ctgaggtttc agacattatg gaaggcacac gccgtgccat
     1081 gcgcgccgcc tataaccgtg agatggacga tttagatgcg catttgccgt ttttagcctc
     1141 ggtcggctcg gtgagtccgt atattggttt gttcggcacg gtgtggggta ttatgaattc
     1201 gttcagaggc ttgtctaacg ttgcgcaagc cacgctcagc caagtggcac cgggtattgc
     1261 agaagcgctc attgccacgg ccattggctt atttgcggca attccagccg tcattgccta
     1321 caaccgtttt gcctcaagtg ttgacaggct ttcagtgcgt tacgaaagct ttatggaaga
     1381 attcaccaac atcttgcaaa gaaagagcta aatcatggcg cgcacccgta aacgccgcat
     1441 gatgaaccaa atcaatgtcg tgccctacat tgatgtcacc ttggtcttgc tggtgatttt
     1501 tatggtgacc gcccccatga caaaccccgg cgtggtggat ttgccaaaag ttgggcaggg
     1561 agcattagtg caaaagcaag tgccgccgat agttgtaact ataaaatcaa atgacaagat
     1621 tgaatttgaa ggtgcggctg tttcatctga tgtacttttg ttaaaagtaa agcaagcatt
     1681 agatgctaac ccgcaaaggg ctgtcgtaat agctgcagat aagaatgtta aatatgatga
     1741 tgtaactagg gtggtgggat tgtttaagca aaacaaagat attaaattag gcttgttgtt
     1801 agcgcctgcg ccatagattt aaataatatg gatttcaata caatgcgtgg cccatctcag
     1861 accacaatgc ttaaaagcta tgaaaaagaa atttcatgga aggcgggcgc gtacgcgatt
     1921 gccgtgcatt tagcgttatt gggcgggcta ttgatttcat ttaattggaa agccgcacat
     1981 ccgatgttga acgtggcgga agtcgagctt tgggagagta ttcccagtag cgcaccgccg
     2041 ccgcccttga ttcaaccaaa agttgaacca aaaattgagc cagccccggt ggtaaaagac
     2101 gtgccagaac caaagccaga ggcgaaggtt gaagaaccaa aagtcgatat tgagcttgag
     2161 aagaaaaaag aacttgaaca gaaaaagctg gaagaaaaag ctgcgaaact taggaaagag
     2221 cgattagccg caattcaggc agatacacta aaagacgaaa agcgtgatag cgaaaagcaa
     2281 ttaaatgatc aaaaagataa agacgctatc aaaaaaattc agcaagatat tgacgctgac
     2341 ggtaatcagc aagcgctggc agcaaaagcc tcggctaatg caggagttat tgatgaatat
     2401 aaaagaaaaa tccaagataa aatcaaaggc aacgtcaata acgaactgtg tggtgacggc
     2461 aagcctgtat taaaatttaa aattgattta ctgccatcgg gcgaactaag tggcagccca
     2521 aaactcacca aaaccagtgg cattgaagcg tgtgatgatg ctgtagagcg cgccattatg
     2581 gcttcgcagc cattgccgtt gcctgaggat gtcgcgttga agtcgcaatt caggaactta
     2641 aatttaactt ttacacctaa taattagcaa tttgttagtg cgcggttctg gatggctttt
     2701 gtaacaaagt gtaaatcgtg tggcatcaaa atggttttgc ggttaaattt acacgaataa
     2761 atatcagggg ctcaatttaa caatcgagac gataaaacat aacaccatga aaatatttag
     2821 atttttagct ttttttactt catttttcgc atcaacattc ttatttactc ccgtcgcaaa
     2881 cgctgctttg gaaattgaaa tcagcggggg tagcgcacag caaattccaa tcgccattgt
     2941 gccttttggg caaggtgagc atgccgctac catgagcggc attattgccg ctgacttaaa
     3001 gcgtagcggt ttgtttcgtg tgcttgaaac tggtggtgtg gctagtcgcc caaccgatat
     3061 tacgcaaatt aaatttgctg agtggtcggc attacaagcc caagccatgt cggttggcac
     3121 cattgagcca caagcggatg gcaagttaaa agtatcgttt cagttggccg atgtgctaaa
     3181 acaaacgcag cttaccagcg gtcaatataa tattagccca agccagctaa gaattaccgc
     3241 acataaaatt gccgatgcga tttatcaaaa acttaccggt gaatcaggta tttttgccag
     3301 ccacattgct tatatcaaca aatcaaaagg ccgctacact ttgcaggtgg ccgatgcgga
     3361 tggtgcgaac ccgcgtacga tgttgtcatc aaaagagccg attatttcac ctgcctgggc
     3421 gccagatggc aaccgcattg cctatgtttc ttttgaaaag aaaaaaccga ttatctatgt
     3481 acaaactctg ggcggacaac gtaccaccgt ggcgagcttc aaaggcaaca acagcgcacc
     3541 tgcttggtcg ccagatggta ataagctcgc tattgtgctc acgcatgggg ctaattcgca
     3601 gttatatacc attaatgcag atggctcagg cttaaagcag gtgactaaga gttctgccat
     3661 tgataccgaa ccgacatggt caaccgatgg gcagtggatt tattttacct ctgaccgtgg
     3721 tggccgcccg caaatttaca aggtttcatc acaaggcggt gaccccaaac gggtgagttt
     3781 tgaaggcgct tataatgtaa gcccgcgttt ttcaccagat ggtaaatcat tagcgatgat
     3841 tcgtaatgat ggcggcagat tccgcgtggc gctgcaagat ttaaacagtg gtcaagtgca
     3901 gttgctaagc gatggcggcc aagatgagtc gcccagtttt gcgccaaacg gacgcgtaat
     3961 attgtatgct acccgtaacg gtggccgtgg cgcattggcg gcggtgtcgg ccgatgggcg
     4021 cgtgaaacag cgcttaaatg agtctggtgg cgatattcgc gagccggctt ggggcccttt
     4081 gattaaatag taatttttag cagcaatgtg tagtcgattc aatttacaat ttttatgtag
     4141 cagtttaagc aatttttagt attttatttt tatggagagc actatgaaca acctgacttt
     4201 taacaaaaaa ctactcagca cattgatgtt ggctttggta ttaaccgctt gtaaaagcac
     4261 accaatgact aaaccttcta ttgaagacaa aagcgtaggc caaaacacaa ccaccaccac
     4321 gccagctgct ggtgcggatg atggctcaac cgttaaagaa gtgtcacttg atgacgccaa
     4381 tgccaatgat gcaggcaacc cattaaaaga tcctaacaat attttgtcaa aacgtaatat
     4441 ctactttgac tttgatagcg atgctgtcaa ggcagaatat cgcccattgg tagaagcgca
     4501 tgcaaaatac ctactaagcc ataaagatgc gcgtgtgatt gtgcaaggca atgccgatga
     4561 gcgtggtaca cgtgaataca acttagccct tggccaacgt cgttcagttg cggtgaaaaa
     4621 agcattgaac gtgttgggtg tgcaagatga acagattgaa accgtgagtt acggtgaaga
     4681 aaaaagcacc ggttgtgctg atgaggcttg tttcagcaaa gaccgccgcg cagatatcgt
     4741 gtatcaaggt gagtaataga aactcggtca gccgctatca agttctgata gcggcacctt
     4801 cttttaattt ttagatgcat gcttaaaata tgatgaaaaa actcaatctg gtcagcttgt
     4861 ttttgctggt gcaacttttt gcgatcaata gccatgcggc tttgttcgat gataaagaag
     4921 cgcgcaaaaa aatcttggaa attgaagcga ccatgacgag tcaacatcag gcgacgcaaa
     4981 atgagctggc taatttgaaa aaaagccagc aagcgcttga gcagcgcgtg attgcgattg
     5041 aggccattac caaaggccag gttttgggcg atatgcaaaa ccatattgat acgctcaatc
     5101 aggaaatcgc ccgactaaaa ggtgaactgg aagaagcaaa ccatcatatt gaggccacac
     5161 agcagcgcca aaaagatttg tatggcgata ccgatgctcg tttgcgtaaa ttagagggtg
     5221 gagatgcggc tgccgcaacc actacagcgc ctgatgccac tgcagcagca gcgtcagccg
     5281 ctacagccac accgaataaa aatacccaag aataccagtt gttagagctt gccaatggct
     5341 tatccagaga ggccaagcat aaagatgctt acaatgccta tgacaaattc ttgaaagact
     5401 acccagatag cgcattggcg cccgaggcta aatatggctt gggttacacg caatttgcgc
     5461 ttaaaaacta taaatcagcc attatcacac agcaaaaagt cattgatttg catccagata
     5521 gcccaaaagt acccgatgca atgctgaata aagccaacag tcaaatccag cttgggcagg
     5581 tggcgaatgc gaaaaaaacg ttgcgtgatt taattgcgaa atttccagag agcgaagtaa
     5641 ctcccacggc acaaaggcgc ttaaaagcgc tggaagccat caaataaaaa caggtttttt
     5701 tcacgtcaga ttcaccctat tttctctcga gagatttttg atgctctgta agcctttatt
     5761 tataaggctc gcagagcagg tcaaaagggt tgtgactatt taagcacata atttttcatt
     5821 tttacgaacc ttgtttgttt gccgattgcg cttatatttt aaacagcctt tggctttcgc
     5881 ggtaaaatag cgccaacgca attgttggca ttttttatca tctctcatgg cacttaaaat
     5941 tcacgaaatt ttctattcgc tgcaaggcga atctacgcgt gttggcttgc caacggtgtt
     6001 cgtacgcctt actggctgcc caatgcgctg cgtatattgc gacactgctt acgcgtttag
     6061 tggcggcagc aatatggaaa ctgctgatat tttggctaaa attgccgaat atggcaccaa
     6121 atatgtgact gttaccggtg gtgaaccgct ggcgcagaaa gaatgcatgg tgctgcttaa
     6181 agcgttgtgc gatgcaggtt acaacgtctc tcttgaaaca ggaggggccg tcgatattgg
     6241 cccagtggac agtcgcgttt cagtcatttt agacattaaa acgccagatt caggtgagct
     6301 taaaaattac ctgtgggaaa atctaaccca ccttaaaaag accgatgagg tgaagttcgt
     6361 tttatgtagc cgcacagatt acgactgggc aaagcagatt ttggtgcaat atcaccttgt
     6421 tgaaaaatgt agcgtattgt tctcacctgt ttatagccaa attaacccaa cggatttagc
     6481 caattgggta ttagccgacc aattgccagt gcgtatgcaa cttcaattac acaaaattct
     6541 ttggggcgag gtgccgggaa aatgaccaaa cgtgctgtgg tattgctttc tggcgggctg
     6601 gattctgcca cggtactcgc ctatgcgcgt agtcttggct ttgaatgcta ttgtttaagt
     6661 ttggattacc atcagcgtca catcgctgaa ctgcatgcgg ccaaaaatat cgccaaatta
     6721 atgaatgcta aagcgcacaa aaccgcgcag cttgatttat ccttatttgg cggctcagcc
     6781 ctcacggatg atgccattgc cgtgccagaa agcgccacaa caggcatccc gattacttat
     6841 gtgccggcgc gcaacacgat tatgctgtca ctcgctttgg cttgggctga agtgcttgaa
     6901 gcgcaagata tttttattgg cgtgaatgcg ctagattatt ctggctaccc agattgccgt
     6961 ggtgaatatg tcaaagcgtt tcaaagcatg gccaatttag caactaaaag cgcggtagaa
     7021 ggcaaaacga ttacgattca cgccccgctg atagatatga ccaaggcgca aattgtgcag
     7081 ttaggtacca agttgggtgt ggattatgcc atgactgttt catgttatca ggctgatgaa
     7141 cacggtgaag catgcggcct gtgtgattct tgccgtctgc gtcgcgatgg ctttttagcg
     7201 gcgggtatag atgatccaac gcggtacaaa gcacttccct aaacattggg cacaaactta
     7261 tttgtaaata aatgcagtgc gacttgataa atagcttgtc aaaagcacaa taaatagcgt
     7321 agaatccgcg cctttctgct ttaaaatttc tattaaaaga gaactaagat tatggttatt
     7381 attcgtttag ctcgtggtgg cgcgaaaaaa actcctttct acaacgtggt tgtagctgat
     7441 tcacgcaatc gtcgcgatgg ccgctttatt gagcgcgtag gtttttacaa tccatctgcc
     7501 aaagcaaatg ctgaaagctt gcgcattgat ttagcgcgcg ttacgcattg gcaaaataac
     7561 ggcgcgcaat tgtcagaaac cgtagcgcgc ttggttaaat tccacgctaa aggcccggag
     7621 cacgcaattg ctgcaaaagt taaagatgct gctaaagccg atgctgctaa agctaaaatt
     7681 gctgctgctg aagctgcaaa agtaaaagct gctgccgatg ctgctaaagc tgaagcggat
     7741 gcaaaagccg ctgaagaagc tgccgctgca aaagaagctg aagccgctgc caaggctgct
     7801 gaagcagaag ctgctgctgc acctgctgaa gaagcaccag ctgaagaagc accagctgca
     7861 gaataaatca aagcactcgg gcgttaaaac gccttggtga attaagttta ttttggaagc
     7921 aagtcattta gttatcatgg ggcgcattgt tgcgccttat ggcgtttttg gttggctaaa
     7981 agtcattccc gatacagaag cgctcgatgg tctgcttgat tacgattcat ggtggctagg
     8041 caaaggcaat gattggcgtg aattgaaggt tgtagaggcc aaaatacaca atgatgtgat
     8101 tgtggtgaag cttgaaggca ttaacgaccg cgacgcagca tttgcatgta agggtaagca
     8161 ggtcgctgtg ccaagagatg cgctcccaga gcctgaagaa aatgaatatt actggtcaga
     8221 cctgattggt ttgcgtgtaa aaaataagca agatattgat tttggcacta tcgtggatgt
     8281 gtttga
//
LOCUS       DAOQSI010000086         8119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_8416_length_8119_cov_5.694940, whole genome shotgun sequence.
ACCESSION   DAOQSI010000086 DAOQSI010000000
VERSION     DAOQSI010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 8119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8119
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8416_length_8119_cov_5.694940"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="PKL42_06620"
     CDS             complement(<1..551)
                     /locus_tag="PKL42_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNU66789.1"
                     /translation="MIHNEFQLSHNTERNAKAARLSISIARTQSEIEQAQRLRYKVFA
                     EEMGAQLLGTGGLDVDGFDQYCEHLIVRDNETNQVIGTYRILSPERAFDAGGYYSAGE
                     FDLTRIAHLFDRTVEVGRACVHQNYRNGGTITMLWAGLAQYMQAHQYEFMIGCGSISM
                     ADGGHGAASLYQRLQDEYLSPLE"
     gene            complement(706..1149)
                     /locus_tag="PKL42_06625"
     CDS             complement(706..1149)
                     /locus_tag="PKL42_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HNU66790.1"
                     /translation="MQLFTIGLISVFLGACASSNSGSVYSRDDARKVQTVKTGVVESV
                     RQVKLEGTKSPVGTVAGGAVGGIAGGSIGHGRGSAVGAVVGAVAGALAGAALEEGVTR
                     KDALEITVKLDGGGLIAIVQEADEAFNIGENVRIIENGSTSRVSH"
     gene            complement(1225..2166)
                     /locus_tag="PKL42_06630"
     CDS             complement(1225..2166)
                     /locus_tag="PKL42_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNU66791.1"
                     /translation="MSTLICGSLAFDTIMVFQDQFKNHILPDKIHALSVAFYVPEMRR
                     EFGGTAGNIAYNLQLLEGNPLIMATVGEDFGTYTTWLHHNKLNTAHIKTIPQSFTAQA
                     FITTDTDDNQITAFHPGAMVESHQNSVNDVKNVKLAIIAPDGRDGMFQHARECFEAGI
                     PFMFDPGQGLPMFNGEELLHFIEMADYLAVNDYESQIIQDKTGLTLLQLAEKVKALIV
                     TLGGSGSHIYADGQRFDIPCVKVEKIVDPTGCGDAYRAGLLYGIARGWDWPTCGRLAS
                     TMGAIKIESRGGQNHKPTRDEIETIYTLALLNDVKLD"
     gene            complement(2270..2836)
                     /locus_tag="PKL42_06635"
     CDS             complement(2270..2836)
                     /locus_tag="PKL42_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNU66792.1"
                     /translation="MISLREKILNTASNLFQTQGINSTGVDTIVAAAGTTKMTLYKYF
                     TSKENLILAVLEKGHQDFQTWLNDNLNASTKKPSEKLQILFDFIEEWVTSPNFNGMGF
                     IKASAEFPSEENAVHQLSSEQSRQFRQYISTLATEANIHDADGLALQLSLLFEGAVQA
                     EQMKRGSGAIKYAKTAAKILIDGAKKAS"
     gene            complement(2886..3674)
                     /locus_tag="PKL42_06640"
     CDS             complement(2886..3674)
                     /locus_tag="PKL42_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66793.1"
                     /translation="MDIFNSEWLIRMLEKSSNTPQGRILNLFDILGDWLSAPNLHIIL
                     AAHISSHDLLTNYLSKQATAMHAASPEILAEHIALIAHNAALQTASEPNGNHLKHAKV
                     AANALILAQTQKQWASPKSIHSKRTIYGIAASFFVVGLTIAWLPTYLQTSSQQNIVVA
                     LDVMRKDNSTEIKTAAFASNSQNLSLSAHEAATMYAKYEQMRNGTCQFPEAIQIPDRD
                     KAVYLENVVGGKLPSNLKDLATANIYLEKVRCNFTPMLMANSVG"
     gene            complement(3701..4111)
                     /locus_tag="PKL42_06645"
     CDS             complement(3701..4111)
                     /locus_tag="PKL42_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HNU66794.1"
                     /translation="MLWIKALHIIFVTSWFAGLFYLPRLFVNHAMVTDEATSARLTLM
                     EAKLYRFMLPLAVLALIFGLWLWLGYGITGRWMHPKLTLVAILIGYHWYCGKLLNDFK
                     HNKNQRSHVWYRWFNEVPVIVLAIVVILVVVKPF"
     gene            4333..>8119
                     /locus_tag="PKL42_06650"
     CDS             4333..>8119
                     /locus_tag="PKL42_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HNU66795.1"
                     /translation="MNAPLPGSISHAEHQPAARLREIPYNYTSFSDREIVIRFLGEDI
                     WQVLNALRAERKTGRSARMLFEVLGDLWVVTRNPYLQDDLLENPKRRKALIDALHHRI
                     AAIDARNTNGTANEKVQKLVAAAKLAVEKFAEDFRQTYDLRKKALSKFSKITRKDNIQ
                     FDGLARVSHVTDATDWRVEYPFVVLNPDTEQEIAALVRTSIELGLTIIPRGGGTGYTG
                     GAIPLTPLSCVINTEKLDRHSGVQMRTLPGVARQVATIECGAGVVTRRAMEAASDAGL
                     EFACDPTSADASCIGGNVAMNAGGKKAVLWGTALDNLASWRMVTPDGEWMEVERLDHN
                     LGKIHDIETARFKISRFAEDMKTLLAEPQVLEIPGPSFRKVGLGKDVTDKFLSGLPGI
                     QKEGCDGLITSATFILHRMPKHVRTIALEFFGNVSNAVPAIVEIKDYLDATAKKEPAV
                     IMAGLEHMDERYIKAVGYATKAARQQRPKMVLIADIASDDENAVGEVASAMVRMCNAR
                     DGEGFIAVSAEARKKFWLDRARTAAIAKHTNAFKINEDVVIPLPRLGDYSDGIERINI
                     ELSIKNKLKLLDALEVFMQGELPLQADEDGNADVAMVKTKQVMALKLLSDLRTRWRLI
                     LDNLDAPVDVLGEAGQAFSHYESLFRAVQSYDLRISWKRELKRVMADIFAGREYQKIL
                     DKCDEIHKQVLKSRVFIALHMHAGDGNVHTNIPVNSDDYTMMQDAHAAVDRVMALAKS
                     LNGVISGEHGIGITKMEFLDQSTIDTFTAYKNKVDPNGHFNKGKLLAGSGLDNAYTPS
                     FGLLEQESIIMEQSAIGEIADDISDCLRCGKCKPVCSTHVPRANLLYSPRNKILGTSL
                     LIEAFLYEEQTRRGISLKHFDEFNDVADHCTICHRCEKPCPVDIDFGDVSIKMRNFLR
                     EQGKKKFNAGTTAGMWFLNAKDPATIKFLRTTMIGWGYKAQNLAHTLAKRFGLLKDKK
                     RPPATVGRAEIKSQVIHFINRPMPKKMQSKTSRAMLGIEDDHMIPVIRNPKKVTEESE
                     AVFYFPGCGSERLFSNVGLATQAMLYEVGAITVLPPGYLCCGYPQTSAGNHDKGQEIT
                     TDNRVLFHRVANTLNYLDIKTVIVSCGTCMDQLQKYEFEKIFPGCRLLDIHEYLMEKD
                     VKLKGITGTRYMYHDPCHTPMKTYKPLEVTNTLMGTDVALNDRCCGESGTFAAARPDI
                     ATQVKFRKQEELESGMAKMGLTVGAPEQPVKILTSCPSCLQGLARYGEDTGITADYIV
                     "
BASE COUNT         2226 a         1786 c         1924 g         2183 t
ORIGIN      
        1 tattcaagtg gcgataaata ttcatcttgt aagcgttggt ataaactcgc cgccccgtgg
       61 ccgccatcgg ccatactgat gctgccgcaa ccaatcataa actcatattg atgtgcctgc
      121 atgtattgcg ccaagccagc ccataacatt gtgattgtgc cgccattgcg gtagttttgg
      181 tgcacacaag ccctgcccac ttctacggtg cggtcgaata aatgcgcaat gcgtgtcagg
      241 tcaaactcac cggcagaata ataaccgccg gcatcaaatg ctctttcagg gcttaaaatg
      301 cggtaagtgc caatgacttg gttcgtttca ttatcgcgta caatcaggtg ctcgcagtat
      361 tggtcaaaac catcaacatc aagcccgcca gtgccaagca gttgcgcgcc catttcttcg
      421 gcaaatacct tatagcgtaa gcgctgtgct tgttcaattt cagattgcgt acgtgcaatg
      481 ctaatgctca ggcgcgccgc ttttgcgttg cgctcggtgt tgtgtgatag ttgaaactcg
      541 ttgtgaatca ttaaaatgct cctcttttga aatcaaagag actttaatga agcaatgtga
      601 acagacggtg aaagaaatat gacgctttat tgacagtgcg cttgacttag aagaaagctt
      661 tggtgatgca tggcacacat caccaaattt gtgatttgga aagttttaat gcgaaacgcg
      721 cgaagtactg ccgttttcaa taatgcgcac gttttcgccg atattaaagg cctcatccgc
      781 ttcttgcaca attgcaataa ggccgccacc atctaattta acggtaattt ccagcgcatc
      841 tttgcgggtc acgccttctt caagcgcggc gcccgccaat gcaccggcta cagcacccac
      901 cacggcgcca acagcgctgc cacggccatg cccaatagag ccgccagcaa tgccaccaac
      961 tgcgccacct gcgactgtac caacaggcga tttagtgcct tcaagtttaa cttgccgtac
     1021 actttctacc acacccgttt taaccgtttg taccttgcgt gcatcgtcgc ggctataaac
     1081 actgccagag tttgagcttg cacatgcgcc aagaaagacg ctaattaaac caatggtgaa
     1141 taattgcatt aatttcactt tgtactccca taaaaattaa gttaaaaatt caaaaataag
     1201 aatgtttaca ctcaagcggt tcgactaatc cagtttcaca tcgttcagca acgcaagggt
     1261 gtaaatggtt tcaatttcat cgcgcgttgg tttgtggttt tggccaccac ggctctcaat
     1321 tttaattgcg cccatggttg aagctaatct accgcaagtt ggccaatccc agcctctggc
     1381 aatgccgtaa agcaagcctg cacggtaggc atcgccacag cctgttggat ctacaatctt
     1441 ttcaacctta acgcagggaa tgtcaaaacg ttgaccgtcg gcataaatat gcgagccgct
     1501 tccccctaag gtgacgataa gcgctttcac tttttcagcc agttgtagta gcgtgagtcc
     1561 tgttttatct tgaatgattt gggattcata atcgttcact gctaaatagt ccgccatctc
     1621 aatgaaatgg agcaattctt caccgttaaa catgggcagg ccttggcccg ggtcaaacat
     1681 aaagggaatg ccagcctcaa aacactcgcg cgcatgctgg aacatgccat cacgcccgtc
     1741 aggggcaata atcgcaagtt tcacgttttt aacatcattt acactgtttt gatgtgattc
     1801 gaccatcgca cccggatgaa atgcggtgat ttgattatcg tctgtgtcag tcgtaataaa
     1861 tgcttgtgcc gtaaagcttt gcggaatggt tttaatgtgc gcggtattga gtttgttgtg
     1921 atgcagccat gtggtatacg tgccaaaatc ttcacccaca gtggccataa tcagcgggtt
     1981 gccttctaat agctgcaagt tgtaggcaat gttacctgcc gtgccaccaa attcacgacg
     2041 catttccggc acataaaatg ccacactgag tgcgtgaatt ttatcgggga ggatatggtt
     2101 tttaaattga tcttgaaaca ccatgatggt gtcaaaagcc agtgaaccgc agatgagagt
     2161 agacataaaa agcgcttgaa gtgtgaaaaa agctattatc ctgcataaag ccgcttaaag
     2221 ccagttggta caagtgccaa gtttagctta agcttagttt attgctgggt caacttgctt
     2281 tttttgcacc gtcaattaag atttttgcgg cagtttttgc atacttaatt gcgcctgaac
     2341 cacgcttcat ttgttcggcc tgtacggccc cttcaaacaa caatgaaagc tgcaatgcta
     2401 aaccatctgc atcatgaatg ttggcttcag tcgctagcgt gctgatatat tgcctgaatt
     2461 ggcgtgactg ctcagaagac agttgatgca cagcattctc ttcacttgga aactcggcag
     2521 atgccttaat aaagcccata ccgttaaagt taggtgatgt gacccattct tcaataaaat
     2581 caaataaaat ttgcagcttt tcgctgggtt tttttgtact ggcattcaga ttatcattga
     2641 gccaagtttg aaaatcctgg tgaccttttt caagcacagc caaaattaaa ttctctttgc
     2701 tggtgaaata tttatacagc gtcattttag tggtgccagc cgccgccaca atcgtgtcta
     2761 caccagtcga gttgattccc tgcgtttgaa acaggttact tgcagtattg agtatttttt
     2821 cacgtaatga aatcatgtga tgattatact aacctgtata gagtgtttgc aatgtttatt
     2881 atgcttcagc ctacagaatt ggccatgagc attggggtaa agttgcaacg tactttttct
     2941 aaataaatat ttgcagtggc taaatctttt aagttggatg gcagttttcc gcccacgaca
     3001 ttttctagat aaacggcttt gtctctgtca ggaatttgaa tcgcctcagg aaactggcaa
     3061 gtgccattgc gcatttgctc atatttggca tacatcgtcg ccgcctcatg tgcgcttaaa
     3121 cttagatttt gtgaattgct tgcaaacgca gcagttttta tttcggtcga attgtctttg
     3181 cgcatcacat ctaatgcaac aacgatgttt tgttgcgaac tggtttgtaa gtatgtgggt
     3241 agccaagcaa tcgtcagacc aaccacaaaa aaacttgcag cgatgccata aatagtacgc
     3301 tttgagtgga tggactttgg cgaagcccac tgtttttgtg tttgcgccaa aataagcgcg
     3361 tttgcggcaa ctttcgcatg cttcaggtgg ttgccatttg gttcagaagc ggtttgaagc
     3421 gcggcattat gggcaatgag ggcgatatgc tctgccagaa tttcagggct agctgcgtgc
     3481 attgcggttg cttgtttgct taaataattc gtcaataagt cgtgagagga aatatgtgca
     3541 gcaagaatga tatgtagatt aggcgctgaa agccagtcgc ccaaaatatc aaaaaggttg
     3601 aggatgcgac cttgcggggt attgctggat ttttccagca tgcggattaa ccactcagaa
     3661 ttaaaaatgt ccatcctatt acctttaaat ttatggtgaa ttaaaaaggt ttaacaacaa
     3721 ccagtatcac gactattgca agcacaatca ctggcacttc attaaaccag cgataccata
     3781 cgtgactgcg ttgattttta ttgtgcttaa aatcgtttaa cagtttgccg caataccaat
     3841 ggtaaccaat aagaatggct accaatgtca gcttaggatg catccagcgc ccagtgatgc
     3901 cgtagccaag ccataaccat aatccaaaaa taagcgctag cacggctaat ggcaacataa
     3961 agcgataaag tttagcttcc atgagtgtta agcgcgctga tgtggcctca tcggtcacca
     4021 tggcatggtt tacaaagagt ctaggtaaat agaataagcc ggcaaaccag ctggtgacaa
     4081 aaataatatg tagcgcttta atccataaca tggcggtata accttgctcg atgatagtta
     4141 actcaaataa agagtttacc ttagtttgta aaaaataatg catgtgagtt tgtgagcaat
     4201 atcacaaatt ttggcaaaat attagttaaa agcctattgc tgaataccca caacatggtc
     4261 gcgcttattc gctgggtttc tgcatgttat aatgcatacc tttaatcgtt tggctgttta
     4321 ttaaacaaga aaatgaacgc acctcttcct ggcagtattt ctcacgcaga acatcaacct
     4381 gcagcgcgct tacgcgaaat tccttacaac tacacttcgt tttctgaccg cgaaatcgtt
     4441 attcggtttc tgggcgagga catttggcag gtgctcaatg cgttacgtgc agagcgtaaa
     4501 acaggccgct cagcgcgcat gttatttgaa gtgctgggtg acttgtgggt agtgacgcgt
     4561 aacccttact tgcaagatga tttgctagaa aatccaaagc gccgtaaagc attgattgat
     4621 gcgctgcatc atcggatagc tgccattgat gcacgcaata ccaatggtac agccaatgaa
     4681 aaagtacaaa aactggtggc ggcggcaaag cttgcggttg agaagtttgc ggaagatttt
     4741 cgccaaacct atgatttgcg taaaaaagcg cttagtaagt ttagtaaaat cacacgtaaa
     4801 gataatattc agtttgatgg cttggcgcgc gtatcacacg tcacagatgc cactgattgg
     4861 cgcgtagagt acccctttgt ggtgctgaat ccggacaccg agcaagagat agcggcgctg
     4921 gtgcgaacaa gtattgagtt agggcttacc atcatcccta ggggcggtgg cactggctat
     4981 actggaggcg ctattccatt aacgccactt tcttgcgtga ttaacactga aaaactagac
     5041 cgccattcag gcgtgcaaat gcgcacattg cctggtgtgg caagacaagt ggcgaccatt
     5101 gaatgtggcg caggtgtcgt aacacgtcgc gcgatggagg cggcgagtga tgcaggttta
     5161 gaatttgctt gcgacccaac ttcagctgac gcgagttgta ttggcggtaa tgtggcaatg
     5221 aacgccggtg gtaagaaagc ggtgttatgg ggtacggctt tagataattt ggcctcatgg
     5281 cgcatggtga caccagacgg tgaatggatg gaagttgagc ggctagacca taatctaggc
     5341 aaaattcatg acatcgaaac agcacgcttc aaaatcagcc gctttgcaga agacatgaaa
     5401 accttgttgg ccgaaccgca agtgctggaa attcctggcc ctagttttag aaaagtcggt
     5461 ttaggtaaag atgtaacaga taagttttta tctggcttgc ccggcattca aaaagaaggc
     5521 tgcgatggtt taattacttc tgcaacgttt attttgcacc gtatgcccaa gcatgtacgc
     5581 accattgcgc tggaattttt tggcaacgtg tcaaacgccg tgcctgcgat tgtcgagatt
     5641 aaagattacc tcgatgcaac agccaaaaaa gagcccgctg tgattatggc gggtttggaa
     5701 cacatggacg agcgctacat caaagcggtc ggctacgcca ccaaggccgc gcgccagcaa
     5761 cgcccaaaaa tggtgttgat tgccgatatt gcctcggatg atgaaaatgc tgtgggtgaa
     5821 gtggcgtccg ccatggtgcg gatgtgtaac gcccgtgatg gcgaaggctt tattgcggtg
     5881 tcagctgaag cgcgcaaaaa gttctggtta gaccgtgcac gcacggcggc cattgccaaa
     5941 cataccaacg catttaaaat taatgaagac gtggttattc cattaccgcg attgggcgat
     6001 tattctgatg gtattgagcg catcaatatt gaactaagca ttaaaaacaa acttaagttg
     6061 ttagatgcct tagaagtctt tatgcaaggg gagctgccac tgcaagccga tgaagacggc
     6121 aatgccgatg tagcgatggt aaaaaccaag caggtgatgg cgcttaaatt actgagtgat
     6181 ttacgtacac gctggcgctt aatacttgat aatctggatg cgccagtgga cgtgctgggt
     6241 gaagcaggac aagcattcag ccattacgaa agtctgttcc gtgcggtgca atcgtatgat
     6301 ttacgcattt cttggaagcg tgaacttaaa cgtgtgatgg cggatatttt tgctggtcgt
     6361 gagtatcaaa agattctcga taaatgcgat gaaattcaca aacaagtgct aaaaagccga
     6421 gtgtttattg cgctacacat gcacgcgggt gatggcaatg tacacaccaa tattccagta
     6481 aactctgatg attacaccat gatgcaggat gcccatgcag cggtggaccg cgtgatggcg
     6541 ttagccaaaa gcttgaatgg tgtgatttct ggtgagcatg gcattggcat caccaaaatg
     6601 gagtttttag atcaatcgac cattgatact tttactgcct ataaaaataa agtggacccg
     6661 aatggccact ttaataaagg taaattgctg gctggttctg gtttagataa cgcctacacg
     6721 ccaagctttg gcttgctgga gcaggaatct attatcatgg agcaatcagc cattggcgaa
     6781 attgccgatg atatttctga ctgcttgcgt tgtggcaaat gtaagccggt gtgcagtacg
     6841 catgtgcccc gcgcgaactt actatacagc ccacgcaaca aaattttagg cacgtcacta
     6901 ttgattgaag cgtttcttta cgaagagcaa acgcgccgcg gcatttcact caaacatttt
     6961 gatgaattta acgatgtggc cgaccattgc acaatctgcc atcgttgtga aaaaccttgc
     7021 ccagtcgata ttgattttgg cgatgtgtct attaaaatgc gcaacttttt acgcgagcaa
     7081 ggtaaaaaga aatttaatgc aggcaccaca gctggtatgt ggtttttgaa cgctaaagac
     7141 cctgccacta ttaagttttt acgcaccacc atgataggtt ggggctacaa agcgcaaaat
     7201 ttagcgcaca cattggctaa acggtttggt ttgttgaaag ataaaaaacg tccaccagct
     7261 acggtgggtc gcgcagaaat caaatcacag gtgattcatt ttattaatcg gcccatgccg
     7321 aaaaaaatgc agtctaaaac ctcacgcgcc atgctgggca ttgaagacga ccacatgatt
     7381 ccggtgattc gtaacccgaa aaaggtaaca gaggaaagtg aggcggtgtt ttacttcccc
     7441 ggttgcggct ctgagcgttt gttctcaaat gtgggcttag ccacgcaagc catgctatat
     7501 gaagtgggcg cgattacagt attgccgccg ggttatttat gctgtggtta cccgcaaacg
     7561 tccgcaggca atcacgacaa aggccaagaa attaccacgg ataaccgcgt gttgtttcac
     7621 cgtgtagcga atacgctcaa ttacttggat attaaaaccg tgattgtgtc gtgcggtacg
     7681 tgcatggacc aactgcaaaa atacgagttt gagaaaatat tccctggttg cagattatta
     7741 gatattcacg aatatttaat ggaaaaagac gtaaaactca aaggcattac cggtacgcgt
     7801 tatatgtatc acgacccgtg ccacacgccg atgaaaacct ataaaccgtt agaagtcacc
     7861 aacacgttaa tgggcactga cgttgcgctg aatgaccgtt gttgcggcga aagtggcacg
     7921 tttgcagcag cacggcctga tattgctacc caagtgaagt tccgcaagca agaagaactt
     7981 gaaagtggca tggcgaaaat gggcttaacc gttggtgcgc ctgagcagcc cgtgaaaatt
     8041 ctcacgtcat gcccaagttg cctgcaaggc ttagcgcgtt atggtgaaga tacgggcatt
     8101 actgcggatt atattgtgg
//
LOCUS       DAOQSI010000087         8089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_8463_length_8089_cov_4.930670, whole genome shotgun sequence.
ACCESSION   DAOQSI010000087 DAOQSI010000000
VERSION     DAOQSI010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 8089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8089
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8463_length_8089_cov_4.930670"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="PKL42_06655"
     CDS             <1..898
                     /locus_tag="PKL42_06655"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HNU66796.1"
                     /translation="QSPIALLPDLANAQHYELPTEFFALCLGQHRKYSSCFWLPETKN
                     LEEAEAFALQLTCEHAELQNGQQILELGCGWGSLTLWMAERFPQSRITAVSNSNSQRE
                     YITQTALARGLNNIQVITCDMNTFSPSTFGIDVMFDRVVSVEMFEHMRNHPVLYGKIY
                     DWLVPGGKFLMHIFVHRLTPYAFEVQGRDDWMSEFFFSGGMMPSDDLPLYFQDKLKLK
                     QRWRWDGTHYEKTANAWLVNMDKQQALITPILEATYGVTEATRWRNRWRIFFMACAEL
                     FGYNQGQEWWVSHYQFERPIEG"
     gene            907..1455
                     /locus_tag="PKL42_06660"
     CDS             907..1455
                     /locus_tag="PKL42_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2878 domain-containing protein"
                     /protein_id="HNU66797.1"
                     /translation="MKPLLNFILFQVSWFACVLGAANSLPWLGVLVTLMVVFWHLFQA
                     KMMRPEIILMLTVLVIGGLFDQALLLTGLLDYAHHGWNSALVPVWILALWLAFASVLN
                     VSLRWMREKYLLGVLFGAVGGPLAYLGAEKLGAVTLHSLDAMLALSIGWAVITPILLL
                     LAKRFDGFVEPQVVLASQGRAS"
     gene            1449..2225
                     /locus_tag="PKL42_06665"
     CDS             1449..2225
                     /locus_tag="PKL42_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNU66798.1"
                     /translation="MMNWQLYFYGLAAISTLSFAGWLMSLVRKNVTHVDSLWSLFLAV
                     AAYSYALNVEVFNARIYLMLFLVTLWAIRLSAYLTWRNWGPHEDHRYAAIRKNNAPQF
                     WLKSLYIVFGLQALLAWIISLPLLGVATSAHGTLTLLDVSGALLFTFGFVWQTVADWQ
                     LSLFKADPSHRGQVLRSGLWRYSRHPNYFGESCIWWGFYLIALAAGAWWSVVSPILMT
                     LLLLKVSGVALLEKDIAERRPDYAIYRQQTNAFLPWWPKK"
     gene            2222..2869
                     /locus_tag="PKL42_06670"
     CDS             2222..2869
                     /locus_tag="PKL42_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6134 family protein"
                     /protein_id="HNU66799.1"
                     /translation="MTSLKQQCYTLSCVLISALFSANSMAREWLFDVYLDQSKIGQHQ
                     FKLSDAGVLTSTAKFNVKVLFINAYQYDHQAVEQWRGDCLEKLEARTVEDKVKTSVKA
                     QLTEQHLLVDDGKKQQTLPRCSMTFAYWNPKIIEQSKLLNPQNAEWLETQFIPLGTEN
                     ITVKNEKINAERYKLEGRFAGKPKLNIELWYKGGSDWVALKSITPEGYTIRYQLR"
     gene            2915..3433
                     /locus_tag="PKL42_06675"
     CDS             2915..3433
                     /locus_tag="PKL42_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HNU66800.1"
                     /translation="MTFLASFLSACATQKLAAIPTVAQVDLPKFMGDWYVIAAIPTLI
                     EKQSYNAIERYELNQDGTIATTFTFNKGALDGEFKTYQPRGFVVEDSGNALWGMQFIW
                     PIKAEYRIAYLNADYTQTIIARNARDYVWIMARTPTISEDDYQALTALIASFGYDLSK
                     LRKVPHSDISKP"
     gene            3475..4419
                     /locus_tag="PKL42_06680"
     CDS             3475..4419
                     /locus_tag="PKL42_06680"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNU66801.1"
                     /translation="MIKKLIKWLDNHAVQIADTPSQQADWRQIDLWRVIPFVLIHLAC
                     FAVFWVGFSWFAVGFAIALYALRMFAITGFYHRYFSHKTFRTSRLFQLLGAIAGATAV
                     QRGPLWWASHHRNHHVNADELEDAHSPVQHGFLWSHLGWFLSNQHFATRTERVKELVR
                     FAELRFLDRFDVLVPLVFAVCIYLFGFWLEVKYPSLQTNGLQLLVWGFAISTVALYHA
                     TFTVNSLAHVWGKRRYATRDHSRNNGWIAFLTLGEGWHNNHHHFPGSARQGFYWWEID
                     LTYYILKLLSTCGLIWDLRTIPKEVRESRKIQSHDHKK"
     gene            4424..5689
                     /locus_tag="PKL42_06685"
     CDS             4424..5689
                     /locus_tag="PKL42_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNU66802.1"
                     /translation="MKIAIIGSGIAGNTIAYHLHWHHDITVYEAENHIGGHTHTHTIH
                     HEGKDYQVDTGFIVFNDRTYPNFIALLDELKVAWQPSAMSFSVHCEKTGLEYNGTTLN
                     HLFAQRSNLFKPYFYQMIRDILRFNKSALELLEAGSEIKLGDYLRAGRYCQKFIDYYI
                     IPMGSAIWSTDAVQMLDFPARFFVRFFHHHGMLTVNNRPQWRTITNGSASYVNALTEP
                     FKHRIQLKTPIESVRRTENSVYVKPKHGEEIAYDYVFFACHSDQALKILSDQTLAETE
                     ILSAIPFQENSIYLHHDATLMPKRSLAWAAWNYHVTKQPLNRVAVTYNMNILQNLQSR
                     EPLLVTLNHVGGINPAKVIKRLTYTHPVYTLAGAAAQARHAEISGKNRTGYAGAYWRN
                     GFHEDGVVSALEALKHFESAIKSGLHNAQ"
     gene            5679..6455
                     /locus_tag="PKL42_06690"
     CDS             5679..6455
                     /locus_tag="PKL42_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HNU66803.1"
                     /translation="MPNSAIYEGVVTHQRFSPKPHGFRYRVFMMYLDLDELPQLFRGM
                     RFWSYESKNLACFKRADYYGNPDVPLKEEIANLVYQATGHAPRGAITMLTNMRYFGHC
                     FNPVTFYYCFEPDGTTLQAIVSHITNTPWGEDYAYVHDFKSEKTIKKTKQGDIAVFKL
                     DKNFHVSPFMPMDIEYDWAFKMEGNQLLVHMKNFEEGKQVFNATLALAKKEMTASSLN
                     KLLLSYPLMTIKVVAAIYLNAFLLWCKRVPFYSHPAQVSK"
     gene            6458..7729
                     /locus_tag="PKL42_06695"
     CDS             6458..7729
                     /locus_tag="PKL42_06695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HNU66804.1"
                     /translation="MSNPVQIQETLTASLRQKSRSKWVANFAKAQILKRLALLQKGNL
                     TIVDGAVQHSFGHTEDIRATIYVHDTRFYGEIAFGGSIGAGEAYMLGYWTTDNLTNVI
                     RMMCLNQAVMDTIEGGYQWFTKPLMQLLHWLNSNTTEGSRKNIAAHYDLGNDFFALFL
                     DETLMYSSAIFNQHTSTLKDASELKLKTICEKLDLQATDHVIEIGTGWGGFAIYAAMH
                     YGCKVTTTTISQQQYALAKSRVTAAGLQDKITLLLDDYRHLRGQFDKLVSIEMIEAVG
                     HRFYDTYFAKISALLKPDGLALIQAITIQDQRYESAKKSVDFIQRYIFPGSCIPSNTA
                     MLNSITRNSDLRLFDLEDIGPHYATTLKRWRENFFDNIEAVRKLGYSEEFIKMWEFYL
                     CYCEGGFAERALGDVHLLLAKPDNRRVALNA"
     gene            complement(7945..>8089)
                     /locus_tag="PKL42_06700"
     CDS             complement(7945..>8089)
                     /locus_tag="PKL42_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FxDxF family PEP-CTERM protein"
                     /protein_id="HNU66805.1"
                     /translation="IYGEISLGSFKGASSNWVAAVPEPETYGMMLVGLALVGFAARRR
                     KNG"
BASE COUNT         2216 a         1653 c         1894 g         2326 t
ORIGIN      
        1 tcaatcgccc attgccctgc tgcctgattt agcaaacgcc cagcactatg aacttcccac
       61 ggaatttttt gcattgtgcc taggacaaca tcgtaaatac agcagctgtt tttggttgcc
      121 agaaaccaaa aatttagaag aagctgaagc ctttgccctg caactgactt gtgaacatgc
      181 cgagttgcaa aacggacaac aaattttgga gttaggttgc ggctgggggt cgctcacgct
      241 atggatggcg gagcgctttc cacaaagtcg cattaccgca gtgtcaaatt caaattccca
      301 gcgcgaatac atcacgcaaa cggcactggc gcgaggctta aacaatattc aggtcatcac
      361 ttgcgatatg aatacgttta gcccgagtac ttttggtatt gatgtaatgt ttgaccgcgt
      421 tgtatcggtt gaaatgtttg agcacatgcg caaccaccca gtgctgtacg ggaaaattta
      481 cgattggtta gtgccaggag gcaagttttt aatgcacatt tttgtgcatc gattaacccc
      541 ttacgcgttt gaagtgcaag gtagagatga ctggatgagc gaatttttct tctctggcgg
      601 catgatgcca agtgatgatt taccgcttta ttttcaggat aaacttaaac taaagcaacg
      661 ctggcgctgg gatggtacac attatgaaaa aacggccaat gcttggcttg ttaatatgga
      721 taagcagcag gcattgatta caccaatttt ggaagcgact tatggggtaa ctgaggcaac
      781 gcgctggcgc aatcgctggc gcatattttt tatggcgtgc gctgaattat ttggctacaa
      841 ccagggtcaa gaatggtggg tttcacacta tcaatttgaa cggcctattg aaggctaaat
      901 tgcaccatga agccattgct taattttatt ttatttcaag tttcgtggtt tgcctgcgtg
      961 ctgggggccg cgaacagcct gccttggttg ggcgtgctgg tgactttgat ggtggtattt
     1021 tggcaccttt ttcaggccaa gatgatgcgg ccagaaataa tactcatgct caccgtgttg
     1081 gtgataggtg gcttatttga ccaagccttg ttgttaacag gtttgcttga ttatgcccat
     1141 catggctgga atagcgcatt ggtgccggtg tggattttag ccttatggct agcgtttgcc
     1201 agcgtactca atgtgagcct gcgctggatg cgcgaaaaat atcttttggg agtgttgttt
     1261 ggtgcagtcg gtggcccgct ggcatattta ggcgcagaaa aattaggtgc agtaacgcta
     1321 catagcttag atgccatgtt ggcgctatct attggctggg cagtcattac gccgatatta
     1381 ttattgctag cgaaacgttt tgatggattt gtcgaaccgc aggtcgtatt agcttctcaa
     1441 ggtagggcat catgaattgg caactgtatt tttatggtct ggcagcaatc agcacactgt
     1501 cctttgctgg ctggctgatg agtttagtaa ggaagaatgt cacccatgtg gatagtcttt
     1561 ggagtttgtt tttagcggtg gcggcttaca gttacgcttt gaatgttgaa gtgtttaacg
     1621 cacgtattta cttaatgctt ttcttagtaa ccttatgggc cattcgctta agtgcatatt
     1681 taacatggcg aaattggggg ccacatgaag accatcgcta tgctgcaatt cgtaaaaata
     1741 atgcgccgca attttggcta aaaagtttat atattgtatt tggtctgcaa gctctgctgg
     1801 cttggattat ttcgctgcct ctgctgggcg ttgcaaccag tgcacatgga acgctcacat
     1861 tattagacgt atcaggcgct ttgttattta cgtttggctt tgtttggcaa acagttgcag
     1921 actggcagtt aagtcttttt aaggcagacc ctagccatcg cggccaggta ttgcgtagtg
     1981 ggctgtggcg ctacagcagg caccctaatt actttggtga aagctgtatt tggtggggtt
     2041 tttatttaat cgcactggcc gctggtgcat ggtggagtgt ggtatcgcca atactcatga
     2101 ccttactttt actcaaggta agcggtgtag cgttgttaga aaaagatatt gctgaacgtc
     2161 ggcctgacta cgccatctat cgccaacaga cgaacgcttt tctaccttgg tggcctaaaa
     2221 aatgacaagt ttgaaacaac aatgctacac gttgagctgc gtattaatca gtgcgctgtt
     2281 ttcagcaaat tctatggcaa gagaatggct gtttgatgtt tatttagacc aatccaaaat
     2341 tggtcagcat cagtttaagt taagtgatgc gggtgtgctg accagcactg caaaatttaa
     2401 tgtgaaagtg ttatttatca atgcttatca atatgatcat caagcagtag agcagtggcg
     2461 gggcgattgt ttagaaaaac tagaagcacg gacggtggaa gataaggtaa aaacaagcgt
     2521 taaggcacag ttgacggaac agcatttatt ggtggatgac ggtaaaaaac agcagacgtt
     2581 accgcgttgt agtatgacgt ttgcctattg gaacccgaag attattgaac agtccaagtt
     2641 gctcaatcca caaaatgcag aatggcttga aacgcagttt attccgctag gcactgaaaa
     2701 tatcacggta aagaatgaaa aaataaatgc tgaacgatac aaattagaag gccgatttgc
     2761 aggtaagcct aaactcaata ttgagctttg gtacaagggc ggtagcgatt gggtagcgct
     2821 taaatcaata accccagaag gttataccat tcgttatcag ctacgttagg atgacttcac
     2881 gatgaaactt aatattttaa agagcgtatt tctaatgaca tttttagcca gttttttaag
     2941 tgcttgtgca acacaaaaac tcgccgctat tccaaccgtg gcgcaggtgg atctgccaaa
     3001 atttatgggt gattggtatg tgattgctgc aattccaacc ttgatagaaa agcaatctta
     3061 taacgcaatt gaacgctatg aattgaacca agacggcacc attgcgacca cgtttacctt
     3121 taacaaaggt gcgctagatg gagaattcaa aacctaccaa ccacgcggct ttgtggtgga
     3181 ggatagcggt aatgcgttgt gggggatgca gtttatctgg cccattaagg ccgagtacag
     3241 aatcgcttat ttgaatgcgg attatacgca gacgattatt gcgcgtaatg cccgtgacta
     3301 cgtgtggatt atggcgcgta cgcctacaat tagtgaggac gactatcaag cgctgacggc
     3361 tttaatcgca tcatttggct atgatttaag caagttgcgt aaggttccac acagtgacat
     3421 atctaaaccg taaccgttta acgcgactca atttatgtag catcattttt aaagatgatt
     3481 aaaaaactca ttaaatggct agacaatcat gcggtgcaaa ttgcagacac accgtcacag
     3541 caggcagatt ggcgacaaat tgatttgtgg cgcgtgattc cgtttgtgtt aatacacctt
     3601 gcctgctttg cagtgttttg ggtgggcttt agctggtttg ccgttggctt tgccattgca
     3661 ctttatgcat taagaatgtt tgcaatcacg ggcttttatc atcgctattt ttcacataaa
     3721 acctttcgca cttcgcgcct atttcagtta ttaggcgcta ttgcgggggc cactgctgta
     3781 cagcgcgggc cgttatggtg ggcatcacat catcgaaatc accatgtaaa tgcagatgaa
     3841 ttagaagatg cccattcacc cgtgcagcac ggctttttat ggagtcattt gggctggttt
     3901 ttatccaatc agcatttcgc aacccgcaca gagcgcgtta aagagttggt gcggtttgcc
     3961 gagctacgtt ttttagaccg atttgatgtg ctagtccccc ttgtgtttgc tgtgtgcatt
     4021 tatttatttg ggttctggct tgaagtgaaa tatcccagtt tacaaactaa cggcctgcaa
     4081 ttgctggtgt ggggttttgc catttcaacg gtggccttgt atcacgccac ctttaccgtc
     4141 aattcactgg cccatgtgtg gggcaagcgg cgctatgcga cgcgtgatca cagtcgaaat
     4201 aacggctgga ttgcattttt gacactgggc gagggttggc ataacaatca tcatcatttt
     4261 ccagggtcag ccagacaagg tttttactgg tgggaaatcg atttaaccta ttacattctc
     4321 aaattacttt caacttgcgg tttgatttgg gatttacgaa ccataccaaa agaagtgcgt
     4381 gaatcaagaa aaattcagtc acatgaccat aaaaaataac ccattgaaaa ttgccattat
     4441 tggtagcggt attgcgggta ataccattgc ctatcacttg cattggcatc atgacattac
     4501 agtgtatgag gccgaaaatc acattggcgg ccacactcac actcacacaa ttcatcatga
     4561 aggtaaagat tatcaggtag atacggggtt tattgtgttt aatgaccgca cttacccaaa
     4621 ctttattgcc ttgttagatg agttaaaagt ggcttggcag ccgagtgcca tgagttttag
     4681 cgtgcactgt gaaaaaactg gtttagagta caacggcaca acattaaatc atttatttgc
     4741 gcagcgcagt aatctattta agccgtattt ctatcaaatg attcgtgaca ttctgcgctt
     4801 taacaaaagc gcacttgaac tgttagaggc aggtagtgag attaagctgg gggattattt
     4861 acgcgctggt cgctattgcc aaaagtttat tgattactac attattccaa tgggctcagc
     4921 gatttggtca accgatgctg tgcagatgtt agatttccca gcacgctttt ttgtacggtt
     4981 ttttcatcat catggcatgc ttactgtcaa caataggccg caatggcgaa ccattaccaa
     5041 tggctcggcg agttatgtaa atgcgctgac tgaaccgttt aaacatcgta ttcagcttaa
     5101 aaccccgatt gaaagtgtgc ggcgcactga aaatagcgtc tacgtgaagc ctaaacatgg
     5161 ggaagaaata gcctatgatt atgtattttt tgcctgccat agcgatcaag cactgaaaat
     5221 attaagcgat cagacgttag ctgaaactga aattctaagt gccatcccct tccaagaaaa
     5281 cagtatttat ttgcaccacg atgcaacgct gatgcctaag cgttcattag catgggcggc
     5341 atggaattac catgtcacca aacaaccttt gaatcgcgtt gcggtcactt acaacatgaa
     5401 cattttgcag aacttgcaaa gtagggaacc tctgctggtc acgctgaatc acgttggggg
     5461 aattaaccca gctaaagtca ttaaacgcct cacttatacg cacccagtat atacattggc
     5521 aggagctgct gcccaggcca gacatgcgga aatcagtggt aaaaatagaa cagggtatgc
     5581 aggcgcctat tggcgcaacg gctttcatga agatggggta gtgagcgcat tagaagcgtt
     5641 gaagcacttt gagagtgcta ttaaatcagg cctacacaat gcccaatagt gcaatctatg
     5701 agggggttgt tacgcatcag cgctttagcc caaaaccgca tggctttcgc taccgagtgt
     5761 ttatgatgta cttagattta gatgagcttc cgcaattatt tcgtggcatg cgtttttggt
     5821 cgtatgaaag taaaaatttg gcatgtttta agcgcgcgga ctactatggc aacccagatg
     5881 tcccgctaaa agaagaaatt gctaatttgg tttaccaagc gacaggccat gcgccacgcg
     5941 gtgcaataac gatgctcacc aacatgcgct actttggaca ttgttttaat cccgtgacgt
     6001 tttattattg ttttgagcca gatggcacaa cactgcaggc gattgtgagt catattacca
     6061 acacgccttg gggtgaagat tatgcttatg tgcatgattt taaatcagaa aaaaccatca
     6121 aaaaaactaa gcagggtgat attgcagtgt ttaagttaga taaaaatttt cacgtctcgc
     6181 cctttatgcc tatggatatt gaatatgact gggcgtttaa aatggagggg aatcaattgc
     6241 ttgtacacat gaaaaatttt gaagaaggta agcaagtgtt taacgctacg ctagccttag
     6301 caaaaaaaga gatgacagcg tcatcattga ataaactttt gctaagttat ccgttgatga
     6361 ctatcaaagt cgttgccgcc atttatttga atgctttttt gctgtggtgt aaacgcgtgc
     6421 ctttttattc tcacccagcg caagtctcaa aataaatatg tcgaatcctg ttcagataca
     6481 agaaaccttg actgccagtc ttaggcaaaa aagtcgctca aaatgggtgg caaattttgc
     6541 caaggcgcaa atactcaaac gcttggcatt gttgcaaaag ggcaatttaa ccattgttga
     6601 tggtgcagtt cagcacagct ttggtcacac agaagacatt cgcgcaacaa tctatgtaca
     6661 tgatacacgt ttttatggag aaattgcttt tggcggtagc attggtgcgg gtgaggctta
     6721 tatgctggga tattggacaa cagataacct caccaatgtg attcgcatga tgtgcctgaa
     6781 tcaggcggtg atggatacca ttgagggcgg ttaccagtgg ttcaccaagc cattgatgca
     6841 gctactgcat tggcttaata gcaataccac agaaggaagc cgcaaaaata tcgccgcgca
     6901 ttatgactta ggcaatgatt tttttgcttt atttttagac gaaacgctga tgtattccag
     6961 cgctatattt aatcaacaca ccagcacatt aaaagacgct tccgaactga aactcaaaac
     7021 catttgtgaa aaactagact tgcaagccac tgaccatgtc attgaaattg gcacaggttg
     7081 gggtggtttt gcaatttacg cggctatgca ttatggatgt aaagttacca ccactactat
     7141 ttcacagcag caatatgcgc tggctaaaag tagggtgacg gctgctggct tacaagacaa
     7201 aatcactttg ctgttagacg attatcggca tttgagaggc cagtttgata agctcgtttc
     7261 aatcgaaatg attgaagcag tcggccatcg cttttatgac acctattttg caaaaatcag
     7321 cgcactactt aaaccagacg gtttggcgct cattcaagcc attaccattc aagaccagcg
     7381 ttatgaaagc gcaaaaaaat cagtcgattt cattcagcgc tacatttttc cgggctcgtg
     7441 cattccatcc aacaccgcca tgctcaacag tattacccga aacagtgatt tgcgtttgtt
     7501 cgatttggaa gatattggcc cgcattacgc cacaacgtta aaacgctggc gagaaaattt
     7561 ttttgacaac attgaagccg tacgaaagct tggctactca gaagagttca tcaagatgtg
     7621 ggagttttat ctttgttatt gcgaaggcgg ttttgcagaa cgcgcactgg gcgacgtgca
     7681 tttattgcta gcaaaaccag acaatcgacg cgttgcttta aatgcttaaa tcggcctatc
     7741 aattcgccga ggatttttta tgcctctgcg agcctttatt tataaggctc acagggcatc
     7801 taaaaagatt gggattaaac tcacgtttaa ttactcattc aatacagtac gattaaaatt
     7861 aaaaagtatt gtttagggtc ataacataag aaagggggct gatgccccct ttcttattga
     7921 tagctcattt gcgcaaagtt taatttaacc gtttttgcgc cggcgcgctg caaaacctac
     7981 caaagccaat cccacgagca tcatgccata ggtttctggt tctggtactg ctgcaaccca
     8041 gttagatgat gcacccttaa aactccctaa cgagatctcg ccataaatc
//
LOCUS       DAOQSI010000088         8069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_8491_length_8069_cov_5.028076, whole genome shotgun sequence.
ACCESSION   DAOQSI010000088 DAOQSI010000000
VERSION     DAOQSI010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 8069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8069
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8491_length_8069_cov_5.028076"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="PKL42_06705"
     CDS             <1..911
                     /locus_tag="PKL42_06705"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNU66806.1"
                     /translation="SVALDVLGFTLMRDVAPGEAVFIDMEGHFYSRQCAENAKLNPCI
                     FEYVYLARPDSVIDNVSVYQTRLDMGKSLADKIKREWSDKKIDVVIPIPDTSRPSALQ
                     LGIALGLDYREGFIKNRYIGRTFIMPGQALRKKSVRQKLNPIGIEFKGKNVLLVDDSI
                     VRGTTSQQIVQMARDAGANKVYFASAAPPVRFPNVYGIDMPSRDELLATGRTDAEICK
                     EIGADDLIYQDLDALVKDIKLSNDDINDFDCSCFDGKYVTGDISEAYLANIEAARGDM
                     TKKQKPTNSSTQMDLNLIDSTEEVLE"
     gene            1447..2634
                     /locus_tag="PKL42_06710"
     CDS             1447..2634
                     /locus_tag="PKL42_06710"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HNU66807.1"
                     /translation="MTTSQYHPETLSVRAGSESTEFGENSEALFLNSSFRFKSAAQAA
                     ARFGGTEPGNIYSRFTNPTVTMFQNKLAALEGADQCVATSSGMSAILACVMGLCSAGD
                     HVIASRSIFGTSVQLFSNILKRWGLEVTFVALTDVAAWQQAVTPKTKLFFIETPSNPL
                     TEVGDIQALATIAHQAGAHLVVDNCFCTPAIQKPLDLGADIIIHSATKYIDGQGRCLG
                     GAVLGSKTLMEPVYGFLRTAGVTMSAFNAWVFLKGLETLHVRMEAHAKRALELALWLE
                     AQPQVARVHYPGLKSHPQHALAMRQQSSGGGIVSFEVKPASGQTAQEAAWALIDATQL
                     ISITANLGDAKTTITHPATTTHSRVSAEARAEAGITDGLVRIAVGLEHVDDLKADLAL
                     LTR"
     gene            2796..5339
                     /gene="gyrA"
                     /locus_tag="PKL42_06715"
     CDS             2796..5339
                     /gene="gyrA"
                     /locus_tag="PKL42_06715"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNU66808.1"
                     /translation="MDQFAKETLPISLEDEMRRSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFAMHELSNDYNKPYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQDFSLRYML
                     VDGQGNFGSVDGDNAAAMRYTEIRMAKIAHELLADIDKETVDFGPNYDGSEHEPLIMP
                     ARIPNLLINGSSGIAVGMATNIPPHNLNEVLDACLALLENPETGIEELIELVPAPDFP
                     TAGIIYGTVGVKEGYRTGRGRVVMRGRTHVEDMDKGSRQMLVVDELPYQVNKKTFVEK
                     IAELVNEKKIEGISDLRDESDKSGMRVVIELKRGEIPEVILNNLYKQTQLQDTFGMNM
                     VALLDGQPRLLNLKQMLEAFLRHRREVVTRRTVFELKKARARGHVLEGLAVALANVDE
                     MIAIIKAAPTPPEAKVGLMAKIWHSPVVEEMLKRAAMEAARPEGLDVNFGLTPNGYKL
                     SDVQAQEILQMRLQRLTGLEQDKIVTEYKEVMDVIADLLDILATPARVTTIITDELTE
                     IRKQFGDKRRSEIVVNAQDLSLEDLITPTDVVVTLSHTGYVKSQPLEEYRAQRRGGRG
                     KQAAATKEDDFIDKMFVANTHDYILCFSSRGRVYWLKVYEVPQGSRIGRGKPIVNLFP
                     LEEGEKINAILSVKEFDENHFIFMTTALGTVKKTALTEFANPRKSGIIAINLDDGDYL
                     IGAEVTNGKNDIVLVSDGGKAVWFSEDEVRQMGRATRGVRGMKLAAKQKVLSLLIADN
                     DQQTVLVATENGYGKRTVLADFRHSGRGTQGVKAIAVSERNGLVVAAKLVNEEDEIML
                     ITTGGVLIRTRVKEIRELGRATQGVTLINLGEGEKLSGLEKIVETDDEE"
     gene            5343..6419
                     /gene="serC"
                     /locus_tag="PKL42_06720"
     CDS             5343..6419
                     /gene="serC"
                     /locus_tag="PKL42_06720"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNU66809.1"
                     /translation="MTQIYNFSAGPAVLPKPVLECAQAEMLDWHGTGMSVMEMSHRGK
                     AFLSILEKTEADFRKLLNVPSNYKVLFLQGGAIAENAAIPLNLLNGKSADYAITGGWS
                     KRSVEDARAYGTINVVTNTEAEQFTYVPDFATWQLNKNAAYLHICTNETLQGVEFDGL
                     PDTHGVPIVADMSSHILSRLVDVGQYGVIYAGAQKNIGPAGLCIVVVREDLLGNASPS
                     TPAVLNWKTQAENQSMINTPPTYSIYIAGLVFEWLLAQGGVEAIEKVNIAKANLLYDA
                     IDSSDFYINNVAVKNRSRMNIPFFLKNESLNDAFLKGAEAQGLMQLKGHRSVGGMRAS
                     IYNAMPIDGVQALVAYMKQFENNH"
     gene            6432..7514
                     /gene="pheA"
                     /locus_tag="PKL42_06725"
     CDS             6432..7514
                     /gene="pheA"
                     /locus_tag="PKL42_06725"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNU66810.1"
                     /translation="MSEKLKQHRDQIDAIDEQILKLVNERARLARQIGSLKDDGVIYR
                     PEREAQVLRRLQANNTGPLSNEAVNNIFRSVMSNCRALEKELAIAFLGPLGTYSEEAA
                     LKQFGLGCGAVVCGSIDEVFRTVEAGQADYGVVPVENSTEGAIGLTLDLLLTSTLKIC
                     GEVTLPIHHCLLSQQTDINNISHVFSHAQSLAQCHEWLHKTLPLAELEAVTSNARAAQ
                     MIHDLVSADGTFAAAIASKRAAEIFNLNILAENIEDDPKNTTRFLVLGRHTVAPSGAD
                     KTSLVMMTKNKPGAMVELLEPLSRHGVSMTKLESRPSKQNLWDYIFFVDVEGHSSDTN
                     VASALKECEARASLIKVLGSYPVAII"
     gene            7565..>8069
                     /locus_tag="PKL42_06730"
     CDS             7565..>8069
                     /locus_tag="PKL42_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66811.1"
                     /translation="MPLSASMLGDSVWNKFSLKFNNSRLEAHYKKYIFPRLLKQSRLS
                     LLLIAMMYELYGVLDYLLAPQNLLSQITFIRSATTFVVLAIFILTFFKVFKKHHQTLL
                     TFALLLSSASLLWKMTLIHQSIFPYYFTGLILLLLWVHVFYILNFSNVFFCTLAILIL
                     SFSAFFAL"
BASE COUNT         2259 a         1646 c         1980 g         2184 t
ORIGIN      
        1 aaagtgtcgc gctagacgtg ctgggcttta ccttgatgcg tgatgtggct cctggcgaag
       61 ccgtatttat cgatatggaa ggccattttt atagccgcca atgtgctgaa aatgcaaaat
      121 tgaacccgtg tatttttgaa tacgtctatt tagcgcgtcc tgattcagtg atagataacg
      181 tttcagttta ccaaacccgt ttagacatgg gcaaaagtct tgccgataaa atcaagcgtg
      241 aatggtcaga taaaaaaatt gatgtagtca taccgattcc cgacaccagc cgtccgagcg
      301 ctttgcagtt gggcattgcg ttgggcttgg attatcgtga agggtttatc aaaaatcgct
      361 atattggccg cacgtttatt atgcccggcc aggcgctacg taaaaaatcg gtacgccaaa
      421 aattaaatcc cattggtatt gagtttaaag gtaaaaacgt tttgttggtg gatgactcca
      481 ttgtgcgcgg gacgacttcg caacaaatcg tgcaaatggc gcgcgatgca ggtgccaata
      541 aagtatattt tgcttcagct gcaccaccag tgcgctttcc aaatgtgtat ggtattgaca
      601 tgccaagccg tgatgaactt ttggcaaccg gcagaaccga tgcagaaatt tgcaaagaaa
      661 ttggtgcgga tgatttaatt taccaagact tagacgcttt ggtcaaagat attaaattaa
      721 gtaatgacga tattaacgat tttgattgct catgttttga tggcaaatat gtcacgggtg
      781 acatcagcga agcctacttg gccaacatcg aagcagcgcg cggtgatatg actaaaaagc
      841 aaaagccaac caattcaagc acgcaaatgg atttgaattt aattgatagt acggaagaag
      901 tcttggagta ataagtggct tacaaaagtt ataaactgca gtcataaatg catgctttta
      961 agcacaaacc tttttcgatt ctctgcgagc ctttatttat aaggctctca gggcattaaa
     1021 aattcctcgg gcgtttctgc tgtgattaag cattaaaaat gttgcttgta tggattaaga
     1081 aaaaacctac ttttacatcg aaatcgtgaa gaagtaatga aaaattcttg acctcacact
     1141 gacttaggca taagatgaaa ttgcgctgat ttgagtgact taacttaaac aaagtcttct
     1201 cagcttgcgg gcgcataata aattcagcta aagcgagtta acaaagaacc cgttttggct
     1261 aatgcttaaa cgggtttttt aatgtcgaac cccttgtaaa agcacttctt gtagcgaatt
     1321 actgcgttac gcggtactca aaatgctcat gtacttcatg tacattgcgc tttttgcgtt
     1381 ccgtgcgcct tgaacttcac tacaataagc cctttttcaa ggagttcgat aagataagag
     1441 atgatcatga caacaagcca ataccatcca gaaacgcttt cagtacgagc cggttcagaa
     1501 tcaaccgagt ttggtgaaaa ctctgaagct ttatttttaa attcgagttt ccgctttaaa
     1561 agcgccgcgc aggcagcggc acgttttggc ggtactgagc cgggaaatat ctattcgcgt
     1621 tttaccaacc caaccgtgac catgtttcaa aataaattgg ccgcgttaga gggcgcggac
     1681 cagtgcgtgg caacctcatc tggcatgtct gccattttgg cgtgcgtaat gggcttgtgc
     1741 agtgcgggcg accatgtgat tgcatcgcgc agtatttttg gcaccagcgt acagcttttt
     1801 agcaatattc taaaacgttg gggtttggaa gtgacctttg tcgcgttaac cgatgtggct
     1861 gcgtggcaac aagccgtgac accaaaaacc aagctgtttt ttattgaaac gccttcaaat
     1921 ccgctcacag aagtgggtga tattcaagcg ctcgcgacga ttgcacatca agcaggtgcg
     1981 cacttggtgg tagataactg cttttgtacg ccagccatac aaaagccact agatttaggt
     2041 gcggacatta ttattcactc tgccaccaaa tatatcgatg gacaaggcag atgtttaggc
     2101 ggggccgtac taggttcaaa aacactgatg gaacctgtgt acggtttttt gcgtaccgca
     2161 ggggttacca tgagcgcttt taatgcatgg gtgtttttaa aagggttaga aacacttcac
     2221 gtgcgtatgg aggcgcatgc caagcgcgcg cttgaattgg ccttgtggct ggaggcgcag
     2281 ccacaagtgg cacgcgtaca ttacccgggg ttaaaatcac atccgcagca tgcgttggca
     2341 atgcggcagc aaagtagtgg cggtggcatt gtgagttttg aggttaaacc agcatcaggt
     2401 caaaccgcac aagaagcagc atgggcgctg attgatgcca cacagttgat ttctattacg
     2461 gctaatctgg gcgatgccaa aaccaccatt acgcatccgg caaccaccac gcacagccgc
     2521 gtttctgctg aagcaagagc tgaggcgggt attacggatg gcttggtgcg cattgccgtg
     2581 ggtcttgagc atgtggatga tttaaaagcg gatttggcat tattaacccg atagattagt
     2641 gcatttttga tgtggcgttt tatagcgcca catcgctagg tgcggtgggc tcgtttaaca
     2701 atctcggtca tcaatcaatc tcaaactagc aagccagaac gctgctaatc atgttaaaat
     2761 cacgcgatag tttttaatta aaataaagca aaaatatgga tcaattcgct aaagaaacct
     2821 tacccattag cttagaagat gagatgcgtc gctcatacct agattacgcc atgagcgtaa
     2881 tcgtaggccg tgccttgcct gatgtgcgcg atggtctgaa gccagtgcat cgccgcgtat
     2941 tatttgccat gcatgagctt tcgaacgact ataacaagcc gtacaaaaag tctgcccgta
     3001 ttgtgggtga cgtgattggt aaataccatc cgcatggcga cacggcggtg tatgacacca
     3061 ttgtgcgtat ggcacaagat tttagcttgc gttatatgtt ggtggatggg caaggtaact
     3121 ttggctctgt ggatggtgat aatgcggccg cgatgcgtta taccgaaatt cgtatggcga
     3181 aaatcgccca tgaactactg gccgatatcg acaaagaaac ggtcgatttt gggccaaact
     3241 acgatggttc agagcatgag ccattgatta tgccagcgcg cattcctaat ctgctcatta
     3301 atggtagttc aggcattgca gtaggcatgg cgaccaacat tccgccacat aatcttaatg
     3361 aagtattgga tgcctgtttg gcattgcttg aaaatccaga aactggtatt gaggaattga
     3421 ttgaattggt gccggcgcca gatttcccaa cggctggcat tatctatggc accgtgggtg
     3481 taaaagaggg ctaccgcaca ggccgcggtc gcgtagtgat gcgcggtcgt acacatgtgg
     3541 aagacatgga taaaggtagc cgccaaatgt tggtggtgga tgaactacca taccaagtca
     3601 acaaaaagac ctttgttgaa aaaattgccg agttggtcaa cgagaagaaa atcgaaggta
     3661 tttcagattt gcgtgacgag tctgataaat ctggcatgcg cgtggtgatt gagctcaagc
     3721 gtggcgagat tccagaagtg attttgaata atctctataa gcaaacgcaa ttacaagata
     3781 cctttggcat gaacatggtg gctttgctgg acggtcaacc gcgcttactc aatctaaagc
     3841 agatgttaga agcgttcctg cgtcatcgtc gcgaggtggt cacacgtcgt accgtatttg
     3901 agttgaaaaa agcgcgcgca cgtggccatg tattagaagg tttagcggtt gcattggcaa
     3961 acgtagatga gatgattgcc attatcaaag ccgccccaac gccaccagaa gctaaagtgg
     4021 gcttgatggc caaaatttgg cactcacccg ttgttgaaga aatgctcaag cgtgctgcca
     4081 tggaagcggc tcgcccagaa ggtttagatg tcaattttgg cttgacacca aatggctata
     4141 aattaagtga tgtgcaagcg caagaaatct tgcaaatgcg cttacaacgc ttaactggac
     4201 tagagcaaga taaaattgtc acagagtata aagaagtgat ggatgtgatt gccgatttgc
     4261 ttgatatttt agccacgcca gcgcgtgtaa cgaccattat taccgacgag ttaacagaaa
     4321 ttagaaaaca atttggcgat aagcgtcgta gcgaaattgt ggtaaatgcg caagatttat
     4381 cactcgaaga tttaatcacg ccgaccgatg tggtggtgac gctatcgcat acaggttatg
     4441 tgaaatcgca accgctggaa gaatatcgcg cacagcgccg tggtggtcgt ggcaagcaag
     4501 ctgctgcaac caaggaagat gactttatcg acaaaatgtt tgttgcgaat acgcatgatt
     4561 acattttatg cttcagtagc cgtggtcgcg tgtattggtt aaaagtgtat gaagtaccgc
     4621 aaggtagccg cataggccgt ggcaaaccga ttgtgaactt gttcccgctt gaagaaggtg
     4681 aaaaaattaa cgccattctt tctgtgaaag aatttgatga aaatcacttt atttttatga
     4741 ccaccgcact tggcaccgtg aaaaaaaccg ccttaactga atttgccaac ccacgtaaat
     4801 caggcattat cgccatcaac ttagatgacg gtgattacct gattggtgca gaagtcacca
     4861 atggaaaaaa tgacatagta ctggtttcag atggcggaaa agccgtgtgg tttagcgaag
     4921 atgaagtgcg ccaaatgggt agagctacgc gcggtgtgcg aggcatgaag cttgcagcaa
     4981 aacaaaaagt attatcattg ctcattgctg acaatgacca acaaaccgtg ctggtagcga
     5041 cagaaaatgg ctatggtaaa cgcaccgtgt tggctgattt ccgccattct ggccgtggta
     5101 cgcaaggcgt aaaagccatt gccgtgagtg agcgtaatgg cttggttgtg gcggcgaaat
     5161 tggtcaatga agaagatgaa attatgctca tcaccacagg cggcgtgttg attcgtacac
     5221 gcgtaaaaga aattcgtgaa ttaggtcgtg caacacaggg cgtcacgttg attaatttag
     5281 gcgaaggtga aaagctctca gggcttgaga aaatagttga aacggacgac gaagaatagt
     5341 ttatgacgca gatttacaat ttttccgcag gcccagcggt attacctaaa ccagtattag
     5401 aatgcgccca agctgaaatg cttgattggc atggcactgg tatgagcgtg atggaaatgt
     5461 cgcaccgtgg caaagcgttt ttgagcattt tagaaaaaac ggaagcggac tttcgtaagc
     5521 ttttaaatgt gccaagcaac tacaaagtgc tgtttttgca aggcggggcg atcgcagaaa
     5581 atgctgcgat tccattgaat ttactcaacg gaaaaagtgc agattatgcc attaccggcg
     5641 gctggagcaa acgtagcgtt gaagatgcgc gtgcttacgg caccattaat gtggtcacca
     5701 ataccgaagc tgaacaattt acctacgtgc cagattttgc tacttggcag ctcaacaaaa
     5761 acgcggccta tttgcatatt tgcaccaacg aaaccttgca aggcgttgag tttgatggct
     5821 taccagatac acatggtgtg ccgattgtgg cagatatgtc ttcgcatatc ctatctcgac
     5881 tagtagatgt tggccaatat ggtgtgattt acgccggagc acaaaaaaat attggcccgg
     5941 caggcttgtg cattgtagtt gtgcgcgaag atttgctggg caatgcttcg ccatccacgc
     6001 cggcggtact caattggaaa acgcaggctg aaaaccaaag catgatcaac acgccaccca
     6061 cgtacagcat ctatatcgct ggcttggtgt ttgagtggtt gcttgcgcaa ggcggggtgg
     6121 aggcgataga aaaagtcaat attgctaaag ccaacttgct ctatgatgcg atcgatagca
     6181 gcgattttta tattaataat gttgcagtta aaaaccgttc gcgtatgaat attcctttct
     6241 ttttaaagaa tgagtcgctg aatgatgcct ttttaaaagg cgcagaggca caagggctaa
     6301 tgcagctaaa aggtcatcgc tctgtgggtg gaatgcgtgc cagtatttac aatgccatgc
     6361 ccattgacgg tgtgcaggca cttgtcgcct acatgaagca atttgaaaat aaccattaat
     6421 tttttaaaaa aatgtcagaa aagctgaagc aacaccgcga tcaaatagat gcaatagacg
     6481 agcaaatact caagcttgtc aatgagcgcg cacgacttgc gcgtcaaatc ggctcgctta
     6541 aagatgatgg tgtgatttat cggcctgagc gtgaagcgca ggtgttgcgt cgtttgcagg
     6601 ccaataacac tggtccgttg tcaaatgaag cagtgaataa tatttttcgt agcgtgatgt
     6661 cgaattgccg cgcacttgaa aaagagcttg ctattgcctt tttagggcct ttgggcactt
     6721 acagtgagga ggctgcgcta aaacaatttg ggctaggatg cggtgcggta gtgtgtggct
     6781 caattgatga agtttttcgt acagtagagg ctgggcaggc cgattatggc gtggtgccag
     6841 tggaaaactc tactgaagga gcaattgggc taacacttga tttgttatta acaagtacac
     6901 ttaaaatttg tggtgaagtg acattgccaa ttcaccattg tttgctttcg caacaaacag
     6961 atatcaataa catctcgcat gtgttttctc atgcacaatc gctcgctcag tgccatgagt
     7021 ggctacataa aacactgccg cttgctgagc ttgaagccgt gaccagtaat gcacgcgcgg
     7081 cgcaaatgat tcatgatttg gtatctgcgg atggcacttt tgctgccgcc attgcaagca
     7141 aacgcgcagc tgaaatattt aacttaaaca ttcttgctga aaacatcgag gatgacccta
     7201 aaaatacgac acgattttta gtactgggca ggcatacagt agccccttcg ggtgccgata
     7261 aaacctcgtt ggtgatgatg acaaaaaaca aaccaggtgc tatggttgag ttgttagaac
     7321 cgctgtcgag gcatggtgtg agtatgacta agcttgaatc ccgcccctct aaacaaaact
     7381 tatgggatta catatttttt gtggatgtgg aaggacattc ttctgacaca aatgttgcgt
     7441 ctgccttaaa agagtgtgaa gcacgtgctt cactgattaa ggtgttgggg tcgtaccccg
     7501 ttgcgattat ttaaatggat taaaaaaatc atttgttgtg aattgatatt ggaaaactct
     7561 tgatatgcca cttagtgcct ccatgttggg cgatagcgtt tggaataagt tttcacttaa
     7621 attcaacaac tcacgcctag aagcacatta caaaaagtat atctttccta ggttactcaa
     7681 gcagtcacgc ctgtcattat tgctgattgc aatgatgtat gaactatatg gcgttttaga
     7741 ctacttacta gccccgcaaa atttactgag tcaaattact ttcattcggt cggcaacaac
     7801 atttgttgtc cttgcgatat ttattctcac tttttttaaa gtatttaaaa aacatcacca
     7861 gacattgctg acatttgcat tgttattaag tagcgcgagt ttgctttgga aaatgacact
     7921 cattcatcag agcatctttc cgtattattt cacggggttg atattactac tgctgtgggt
     7981 gcatgttttt tatatcctta atttttcaaa tgtgtttttt tgcacattag ccatactcat
     8041 actgtctttc agtgcgtttt ttgctttat
//
LOCUS       DAOQSI010000089         8052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_8522_length_8052_cov_3.484682, whole genome shotgun sequence.
ACCESSION   DAOQSI010000089 DAOQSI010000000
VERSION     DAOQSI010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 8052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8052
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8522_length_8052_cov_3.484682"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1347
                     /locus_tag="PKL42_06735"
     CDS             <1..1347
                     /locus_tag="PKL42_06735"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66812.1"
                     /translation="PNAQVTGGLGIYQFLLVNPAPGACQGSGIYILQVVQPAGYLPPA
                     STIIPPTAGNHTPVADAGDGLVDDIQEQSVPPTGAEPTTYYFGLNLTLGGVGVVNNHI
                     PLDPILNNAFIVTKTGNKSVAEIGETVMYTVQARLLQGLTVTELQLIDNLPAGFRYIP
                     GTATYATGSNAATALADPLPVGNKGPQLTFQIGPFAPAATLVTVTYKVRVGVGAMQGD
                     GINRVRGVAPGGLVSNTAQYKVKVTGGVFTNDACVAGKVFVDCNNNHIQDAEELGIPG
                     VRMYMEDGTYFITDVEGKYSYCGISPKTHVLKVDSLTMPRGSRLTATSNRNAGDGNSL
                     FLDTKNGELIRADFAEGSCSNTVLEQVKARRTGGEVRAPETEKKGGPAIKFEGKSPRY
                     PKQGTDSANQKLVKPRGGAGDSPVSNTVNDTPVQGLPDASGNTRGNNLRDQKGEGK"
     gene            1344..4880
                     /locus_tag="PKL42_06740"
     CDS             1344..4880
                     /locus_tag="PKL42_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66813.1"
                     /translation="MRQLDLFSQNQLSAACEHSSEQVTKASSSNVRNMCMKQQDNNVR
                     ASVFGVMCMRAVVIAITSALTANVAYADDEVRFDGLAGSSAASEVSHLVNPQTPETDP
                     DVKQPLSHFAGTSQNGTVDYKANEAVGKIAVEIDKDGLPADGQSAVEVTVKLSDLNAQ
                     NLEGVAYITVETSGGRVLLPGQSTDELGPEGGDLDKVTPGTQVKVENGLATFKLLAPA
                     EPQDVNVRITAGNVSAAGTVSFVPELREMVAAGLTEGIISKRNISAGSMSPNRREDGF
                     EQQIRRWSNQFQNGKANAAMRTAFFIKGKIKGDALLTAAFDSDKETRARLLRDIRPDE
                     FYPVYGDSSIHGFDARSSDRLYVRIDKNKHYALYGDFSTGSGFSQQTGGGNVASLQLR
                     NLGQYNRTATGFRGHFEQGNVLGNAFLVRDNLKQAIEEYPGNGTSGPFAVKNNSALEN
                     SEKVEIVIRDKDQLNVVKEVIALSRFEDYVFEPFSGRILLKTPLMALTPNGDRQSLRI
                     TYEVEQEDGEQFWVGGIDGQIKITNNIEVGGAWVEDRNPLSPYRLQSVNTGIKLGENT
                     MLVGEIARSDSTQYQYGTNLFSTPTGQAGEIEQSNSGNAYRVEIVSKSAQDALKPWNA
                     KAWWARSDADFDNSATGYTQGKGEAGARAKIQLSEFIGVYGEAIRSEDRALAGEAVRT
                     GEKLGLTAKVTDKLTLDGFVRHISEDGNLAPQALIGSNNAPLGGTLNQTGGFYGLGQD
                     QNGFASTGIASRGPGLSDSLDATTVGLGAHYQATEKLNLDALFERGGENQKRIVLGSQ
                     YQIAERTKLYARAETQTGLASSYSLDAADRSTSFSAGVDTSYMKGGNLFSEYRLRDAL
                     SGQQADIRDMQLANGARNVWDISEGVTVNTGIEYLKIFKGEQQNAMALTGGMDYNKNP
                     LWKASGRIEFRRLFDNNAVSGNQSSNQWLNTLSFARKLDRDWTLLTRNYLLYTRNNED
                     ASGLAKGNTFQDRFQIGAAWRPVDHNQWNGLARYEYKKMRDDSLAKDATGVSTGEDYR
                     THIASAHMDYHPSRPWWMTGRVAAKRTTDDTLPSDKTYTAYLVSGRTVYDITEKWDLG
                     VMGSVLYSPQGSATQWAAGLEAGYQLQTNLWASAGFNWSGFHDRDLSGSDYTAQGFYV
                     RLRFKFDENLFSGKSEEVNRTLERTDSAPEIN"
     gene            4972..6042
                     /locus_tag="PKL42_06745"
     CDS             4972..6042
                     /locus_tag="PKL42_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNU66814.1"
                     /translation="MKKTSTSFKAFNIKTISMLVGTLIMSSTVQAETSAEPMLNEAKD
                     RITDQAIRADQATYTATQARIHALNETGIPVADYNLSKAQCWLDVSLHEYTRNDRSAF
                     PQEALSQSAGILTALEQNTTPNPAEQTPLVNNADKLREDLWAKAGKLMQAPGKTCYAQ
                     KVACAEVELVHAGNEHKQQGWRHAKPYVQIAEDLLAEADAIGDSCLPPPAPVAATPVA
                     APIETVEEFSIAADALFKFNKSSGDDLLPAGKQTLDDIAAKINDGYVVIDSIKLTGYT
                     DRLGSESYNQKLSERRAQTVKAYLQSKGFTHVIETYGKGEADQVAECGTSTKPTKALT
                     DCLQPNRRVVVEVKGVKKGTAQ"
     gene            6322..6411
                     /locus_tag="PKL42_06750"
     tRNA            6322..6411
                     /locus_tag="PKL42_06750"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6356..6358,aa:Ser,seq:tga)
     gene            complement(6682..7647)
                     /locus_tag="PKL42_06755"
     CDS             complement(6682..7647)
                     /locus_tag="PKL42_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66815.1"
                     /translation="METIEQRRAYFKAEGDVALQEWYGHLPENCEMYKVASLVVASVV
                     EYFVHPKPELESLYFSNVLISTKDFLDQLRSDLNPINPSLTHPNGGTFLYKKDYYLER
                     ADNTQSPESWLSLKIKNLCYSEFAVRDLLSKGKESVDNWPIEKLNYTCSLYFQKPWLH
                     CAAIDRLLINAMIFQSVMEFCEYLTCFSLNRYTKEYEIRKRQRKNSHLIPFTFLIVFI
                     LGIGFASGYGWLTLAGIIILFAIKYTFNDFLPTPNSQAIEIDANAHLENLFRLFTLSH
                     KIVSISYFREQLTIIDSRFTKVPDEIFSILDNASARGLVLLQDPL"
     gene            complement(7656..>8052)
                     /locus_tag="PKL42_06760"
     CDS             complement(7656..>8052)
                     /locus_tag="PKL42_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66816.1"
                     /translation="WYTRIRVAVHLGVSLLAGVMATDNHGLGIGIPAAIGTWTMLKML
                     SWTSTIKTQWMANPIPLRDYELLVSQIASINVMLLDTRISPEFIRQRLLSVAELGAMW
                     PTPLIALVERAAQRNPVSWGLSFSVRYRY"
BASE COUNT         2360 a         1646 c         1930 g         2116 t
ORIGIN      
        1 ccaaatgcac aagtgacggg tggattgggc atctatcaat tcttgttggt gaacccagca
       61 cctggcgctt gccaaggtag cggaatttac atcttgcaag tggtacaacc tgcgggttac
      121 ctgccgccag catcaacgat tattccacca acagctggta accatacccc agttgccgat
      181 gcaggagatg gcttggtgga tgatattcaa gaacagagcg ttccacccac aggtgcggag
      241 ccaacgacct attactttgg cttaaactta accttaggtg gcgtaggtgt tgtgaacaat
      301 cacattccgc ttgatccaat tttaaacaat gcgtttattg tgacgaaaac aggcaataaa
      361 tcggtggcag aaattggtga aactgtgatg tacacagtgc aagcaaggct gttacaagga
      421 ctgactgtta ctgagttgca gttgattgat aacctacctg ctggcttccg ttatattcca
      481 ggcactgcaa catatgctac tggcagcaac gccgccacag cattggctga tccgctgcct
      541 gtgggtaaca aaggcccaca actgaccttc caaattggtc catttgcccc tgctgctact
      601 ttagtgacgg tgacatataa agttagagtt ggcgtaggcg ctatgcaggg cgatggtatt
      661 aaccgtgtac gaggtgtggc acctggtggg ttagtctcaa atactgcgca atacaaagta
      721 aaagtcactg gtggtgtgtt taccaatgac gcttgcgtag caggtaaggt gtttgtagat
      781 tgcaacaaca accacattca agatgctgaa gagcttggta ttccaggggt gcgcatgtac
      841 atggaagatg gcacttactt tattacagac gttgaaggca aatacagtta ctgcggtatt
      901 agcccaaaaa cccacgtact caaagtagat agcttgacca tgccgcgtgg cagccgatta
      961 accgctacca gcaaccgtaa tgcgggtgat ggcaatagct tgttcttaga tactaagaac
     1021 ggcgagttga ttcgtgctga ctttgcagaa ggcagttgct ctaatacggt actagagcaa
     1081 gtaaaagcac gtcgcacagg tggtgaagtg cgcgcgcctg aaactgagaa aaaaggtggg
     1141 cctgccatta agtttgaggg caaatctcca cgctatccaa agcaaggcac ggatagtgca
     1201 aatcagaaat tggtaaaacc gagagggggt gcaggcgact cgcctgtgtc aaacacggtg
     1261 aatgatacgc cagtgcaagg gttgccagat gctagcggca atacacgtgg taataacctt
     1321 cgtgaccaaa aaggagaggg caaatgagac aacttgacct ttttagtcag aaccaattgt
     1381 cagccgcctg tgagcattcc agtgaacaag tcacaaaagc gtctagttct aatgtgagaa
     1441 atatgtgtat gaagcaacaa gataataatg tccgtgcgag tgttttcggt gtaatgtgta
     1501 tgcgtgccgt ggtaattgcc atcacgagtg ccttaacagc aaacgtagca tatgcagatg
     1561 atgaagtgcg ttttgatggc ttggctggta gcagtgctgc cagtgaagtt tcacatcttg
     1621 ttaatccaca aacccctgaa actgacccag atgtaaaaca gccattaagt cattttgcag
     1681 ggacgagcca aaacggtact gttgattaca aagccaatga ggcggtaggt aaaattgccg
     1741 ttgaaattga taaagatggg ttacctgctg acggacaaag tgcggtagaa gtgaccgtaa
     1801 aactatctga tttgaacgca cagaatcttg aaggtgttgc ttatattacg gttgaaacta
     1861 gcggtggccg cgtattgttg ccgggacaaa gcaccgatga gctaggccca gagggtggcg
     1921 atttagataa agtaacgcct ggcacacaag tgaaagtaga aaatggctta gcgacattta
     1981 agttgctagc acctgccgag ccgcaagatg tgaatgtacg tattacagcc ggtaatgtaa
     2041 gtgctgccgg tacggtgagt tttgtgcctg agctgcgaga gatggttgct gccggcttga
     2101 ctgagggcat tattagcaaa cgtaatattt ctgcgggttc aatgtcccct aaccgtcgtg
     2161 aggatgggtt tgaacagcaa attaggcgtt ggagtaatca gttccaaaac ggtaaagcca
     2221 atgccgctat gcgtacagcg ttttttatta aaggcaaaat taaaggtgat gcattattaa
     2281 cagccgcatt tgactcagat aaagaaacac gcgcacgttt attgcgtgat atacgcccgg
     2341 atgagttcta ccctgtatat ggggatagtt ctattcatgg gtttgatgca cgctcgagtg
     2401 accgtttata tgtgcgtatt gataaaaata aacactacgc gttatatggt gactttagta
     2461 caggaagtgg ttttagccag caaacggggg gtggcaatgt tgccagtttg caattgcgta
     2521 atttaggtca atataaccgt acggccacag gttttcgtgg ccattttgag caaggtaatg
     2581 ttttaggtaa tgcgttttta gtgcgtgata acttaaaaca ggcgattgaa gagtaccctg
     2641 gaaatggtac ttctggccca tttgctgtca aaaacaactc ggctttagaa aactctgaaa
     2701 aagttgaaat tgtcattcgt gataaagatc aacttaatgt agttaaagaa gtcatcgcgc
     2761 tatcacgctt tgaagattat gtgtttgagc catttagcgg ccgcatttta ctaaaaacac
     2821 cactcatggc actcacccca aatggtgata gacaatcgct acgtattacc tatgaagttg
     2881 agcaagaaga tggcgaacaa ttttgggtag gcggtattga tggccaaatt aaaattacca
     2941 ataacattga agtagggggt gcttgggttg aagacagaaa tccgctttca ccttaccgct
     3001 tgcaaagtgt caatacaggt atcaagttgg gtgaaaatac catgctggtg ggtgaaattg
     3061 cgcggtctga ttctacgcaa tatcagtatg gtaccaattt attttcaaca ccaaccggtc
     3121 aagccggtga aattgagcaa agcaacagtg gcaatgcata ccgtgtggaa attgtaagca
     3181 aaagcgcaca agatgcactc aaaccttgga atgcaaaagc ttggtgggca aggtcggatg
     3241 ctgattttga taatagcgcc actggttaca cgcaaggcaa gggtgaggcg ggcgcaagag
     3301 ctaagattca acttagcgaa ttcattggtg tttatggtga ggccattcgt agtgaagacc
     3361 gtgcattggc tggtgaagcc gtgcgtacag gtgaaaagct tggattaacg gctaaagtga
     3421 ctgataaatt aaccttagat ggctttgtgc gccatattag cgaagatggc aatttagcgc
     3481 cacaggcgct gattggcagc aataatgcac cacttggcgg cacactcaac caaacaggtg
     3541 gtttttatgg cttaggccag gaccaaaacg gttttgcatc tactggtatt gcaagcagag
     3601 ggccaggttt atccgatagc ttggatgcca cgaccgtagg tttgggtgcg cattatcagg
     3661 caacagaaaa acttaacctt gatgcgttat ttgagcgtgg tggtgaaaac caaaaacgca
     3721 ttgtgctagg ctcacaatac caaatagcgg agcgtacaaa gctgtatgcg cgtgctgaaa
     3781 cacaaactgg tttggcttca agttattcat tagatgcggc tgaccgtagc acgagcttta
     3841 gcgctggggt tgataccagc tatatgaaag gtggcaactt atttagtgag taccgtctac
     3901 gtgatgccct ttctggccag caggcagata tacgcgatat gcaacttgcc aatggcgcgc
     3961 gtaatgtgtg ggatatttct gaaggcgtga cagtaaatac tggcattgag tacttaaaaa
     4021 tattcaaggg tgagcagcaa aatgccatgg ccttaacggg tggcatggat tacaacaaaa
     4081 atccactttg gaaggcttca ggcagaattg agttcagacg tttatttgat aacaatgcgg
     4141 taagtggcaa tcaatcaagt aatcaatggc tcaatacgct ttcgttcgca cgcaagctag
     4201 accgcgattg gaccttgctc acacgtaact acttgctcta tacgcgcaat aatgaagatg
     4261 ccagtggcct tgcaaaaggt aacacttttc aagatcgctt ccagattgga gcagcgtggc
     4321 gtccagttga ccacaatcaa tggaacggtt tagcgcgcta cgagtacaaa aagatgcgcg
     4381 atgattcgct ggcgaaagat gccacaggcg tatcaacggg tgaagactac cgtacgcata
     4441 ttgcatcagc acacatggac tatcatccga gccgtccgtg gtggatgact ggccgtgtag
     4501 cggcaaaacg tacgacggat gacaccttgc cctcagacaa aacctatact gcctacctag
     4561 tgagcggtcg tacggtgtat gacattactg aaaaatggga cttaggtgtg atgggcagtg
     4621 tgctctacag cccgcaaggc agcgcaacac aatgggctgc agggctagag gcaggttatc
     4681 aattgcaaac caatctttgg gcctctgcag ggtttaactg gtcgggcttt catgaccgtg
     4741 atttaagtgg ctctgattac acggcacaag gtttttatgt gagattacgc tttaagtttg
     4801 atgagaattt attctctggc aaaagtgaag aagtgaatcg aaccttagag cgcaccgata
     4861 gtgcgccaga gattaactaa ttgcaggttt gtaaaaacca tgacgaccac cttaaccatt
     4921 attgcaacat taaatacgaa ataaattcta ggctaaactg gacttaatca aatgaaaaaa
     4981 acatctacaa gctttaaagc atttaacatc aaaacgatta gcatgttagt cggcacttta
     5041 atcatgagta gtacagtgca agcggaaacc tcggctgagc caatgttgaa tgaggcaaag
     5101 gaccgcatta ctgaccaagc tattcgtgcg gatcaagcca cttacacggc aacgcaagct
     5161 agaattcacg cattgaatga aactggcatt ccagttgccg attacaattt atccaaggcg
     5221 caatgctggc tggacgtatc attgcacgaa tatacacgca atgaccgttc tgcatttcca
     5281 caagaagcac ttagtcaatc agctggcatt ttgacggcac ttgagcaaaa cacgacgcca
     5341 aacccggccg aacaaacccc attggttaat aatgctgata aattgcgcga agacctgtgg
     5401 gcaaaggctg gcaaattgat gcaagcgccg ggcaaaactt gctatgcaca aaaagtagca
     5461 tgtgcagaag ttgaactagt gcatgctggc aatgaacaca agcaacaagg ttggcgtcat
     5521 gctaaacctt atgtgcaaat cgctgaagat ttactcgctg aagcagatgc gataggcgac
     5581 agttgcctgc cacctccagc gcctgttgcg gcaacgcccg ttgcagcccc cattgaaacc
     5641 gttgaagaat tcagcattgc ggccgatgca ctgtttaaat ttaacaaatc atcaggggat
     5701 gacttattgc ctgctggcaa acaaacgctg gatgatattg ccgctaaaat caatgatggc
     5761 tatgtcgtga ttgatagcat taaattaacg ggttataccg accgccttgg ttcagaaagc
     5821 tacaaccaaa aactctcaga acgtcgcgca caaacagtta aagcatactt gcagtcaaaa
     5881 ggctttacac atgtgattga aacttacggc aaaggtgagg ctgaccaagt cgcagaatgc
     5941 ggcacatcaa ccaagccgac aaaagccctc actgactgct tacaacccaa tcgtcgcgtt
     6001 gtggtagaag taaaaggcgt taaaaaaggc actgcacaat aaaggtttca tctcaaaaaa
     6061 agcatgtgtt tttaattaat tcctaacaca acctttttcg atgctctgtg agcctttatt
     6121 tataaggctt ccagagcatt tattttttct cgagcattta ttgcgtgctt tttttaaatt
     6181 tttgtacaca atttatagcg tattagaaat ggcaaagtta cttaaaaata tctttttgca
     6241 taaatttaag ctggttaata ttcaaagtga atattttttt gctataatca cgccttcatt
     6301 tgcaagcaag caaatttaga cggaagagtg gcagagcggt ttaatgctac agtcttgaaa
     6361 actgtcgtgg gtgcgagccc accgtgagtt cgaatctcac ctcttccgcc aagaatatcc
     6421 atgaaagcct tatgtctact tgatgtaagg tttttttgtt tgtacagttc ttaccgtagt
     6481 tcttacccga ttgatttggt tacatcgaaa atcccaaata tttacttatg tttaatacat
     6541 gtgctatatg gcggcatggg ggggctatgt tgtcggatgt ggggtggtgg tcgacagtgt
     6601 ctttagctac atatgacatc tacgcttata taatgaaaca ctcacgaacg cttgtagata
     6661 caggtcagag ttgtaacgtt gctataatgg atcctgcagc aaaactaggc ctcttgcaga
     6721 agcattatca agaattgaaa aaatttcatc tggaactttt gtaaatctac tatcaattat
     6781 agttagttgt tctctaaagt aagagatgct tacaattttg tgggatagcg taaataatct
     6841 aaagagattc tcgagatgag cgttagcatc aatttcaatt gcttgtgaat taggggtggg
     6901 tagaaaatca ttaaaggtat acttaattgc gaataaaatt atgattcctg caagcgtcaa
     6961 ccaaccatat ccactagcga aacctattcc aagaataaag acaatcagga aagtaaacgg
     7021 tattaggtga gaattctttc tctgtcgctt acgaatttcg tattcctttg tataccgatt
     7081 gagggaaaag caggttaggt actcacaaaa ttccattaca gactgaaaaa tcatggcatt
     7141 aatgagaagg cggtcaattg ctgcgcaatg tagccatggc ttttgaaagt aaagactgca
     7201 tgtgtagtta agtttttcta ttggccaatt atcaacactt tccttgcctt tggaaagtaa
     7261 gtcacggact gcgaattcac tgtagcatag attttttatt tttaatgaca gccatgattc
     7321 tgggctttgt gtattgtccg cgcgttctaa gtaatagtct tttttatata aaaaggttcc
     7381 accgtttggg tgtgtaagtg atggatttat tggattgagg tcagaacgca gttgatccag
     7441 aaagtctttt gtactaataa gcacattact aaagtagagt gattctaact caggctttgg
     7501 atggacaaaa tattcaacaa cgcttgcaac caccaatgat gcaactttat acatttcaca
     7561 attttctggg agatgaccat accattcttg cagtgccaca tcaccttcag ccttgaaata
     7621 ggctctacgt tgttctattg tctccatttt gacccttaat aacgatatcg aacggaaaat
     7681 gataatcccc atgaaacggg attcctttgt gctgcacgtt ccacaagtgc aatcaaaggc
     7741 gttggccaca tcgcccccaa ttcagctacg gacaaaagtc tttgtcggat gaactcggga
     7801 ctaattcgcg tatcgaggag catgacattt atagatgcta tttgcgaaac aagcaactcg
     7861 tagtcacgca atggtattgg gttagccatc cattgagtct ttatggtgct agtccagctc
     7921 agcatcttta gcatcgtcca agtgccgata gcggcgggta tacctatacc aagcccatgg
     7981 ttatcagttg ccataacacc agcaagcaaa gacacaccta aatgaactgc taccctgatt
     8041 cgtgtgtacc aa
//
LOCUS       DAOQSI010000090         7991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_8634_length_7991_cov_4.378780, whole genome shotgun sequence.
ACCESSION   DAOQSI010000090 DAOQSI010000000
VERSION     DAOQSI010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 7991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7991
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8634_length_7991_cov_4.378780"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PKL42_06765"
     CDS             <1..288
                     /locus_tag="PKL42_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase B"
                     /protein_id="HNU66817.1"
                     /translation="NQARVADKATVFSTSTRNFDNRMGRDARVYLGSAELAAVCAKLG
                     RMPTHAEYLETVGNKLSNTAEIYKYLNFDQMTEYKGAFDKVKKVIPIAEVI"
     gene            349..792
                     /locus_tag="PKL42_06770"
     CDS             349..792
                     /locus_tag="PKL42_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66818.1"
                     /translation="MMSEQTPPKETRVEFVEISPEETLFEKPKRGSWHKMLPLLLLLA
                     VLVWTGLYFGLDNRVVASGAVVYGILTSAFAWLVGVIGLIPIIGPLIVKVLSIGVIWL
                     LNAIGYLVSYIAIKRGYSKDVLTYRGLTIALITGIVIGYIIGHYV"
     gene            814..1959
                     /locus_tag="PKL42_06775"
     CDS             814..1959
                     /locus_tag="PKL42_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HNU66819.1"
                     /translation="MLPLLDLTSWLYHLKHHVRKLTDFFPAIFFFGGFLWDALTIGRN
                     VAASDLLIFSGYWLVAGLILFIISRPSYILIGFPQPTTQSKSRYFKWLKGLLSRGYAR
                     FHYPQLPYFLLQFIFGNLLSALFILYFKSASHWLAWLLAFLLGVMLVANEYLEDEYKQ
                     FTLSWALFGFCAMLLFNFCLPFMVGSMHAIWFYLSTLLGAGAAYWLYKKTPNHLGSIA
                     PVGVIAALLMLAYAADMIPPVPLVKRDIAVGYALNKVEGSYQLSQQPASWWEFWQNTS
                     HDLQVIPGQRVYCFSSVFAPSGLKARLYHRWQHYDSKQGWRTMSRIGFTLSGGRYDGF
                     RGYTYKQQLQAGEWKVSVETENEKTVAVQEFTITEVKTAPPVVMMKY"
     gene            complement(1984..3228)
                     /locus_tag="PKL42_06780"
     CDS             complement(1984..3228)
                     /locus_tag="PKL42_06780"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HNU66820.1"
                     /translation="MADLRRVVITGFGLVSPLGNTVEALKENAFAGKSGIKPFMGDFA
                     ELLNCKVTAQAEFDPANYFSKKSDYAILDRNGQMALHASIEAVKHANLTLNDALKKQS
                     GVYIGTGMGGILSVEDGYQTLYKNGENRLKPYTVLMCMYNSAASAIATHFQVSGPNLT
                     FTTACSSSAVAIGQAYKDIRLGDIDVALAGGTDAILAYASIKSWEAIRTLADADAVDI
                     SASCKPFSADRSGLVIGEGAATLVLEEYEHAKARGATIYAEIVGYGAANDYQHIAVPS
                     VEGQAATMAAAMKNSGLQTRDIQYINAHGTGTKFNDETETNAIKQVFGEHAQRLAISS
                     TKSMHGHLMGAAGALEAIITTLAVYHNELPPTINLKNPDPKCDLDYVPNVSRKVERLE
                     YAMSNSFAFGGTGATLIFKKTN"
     gene            complement(3231..3500)
                     /locus_tag="PKL42_06785"
     CDS             complement(3231..3500)
                     /locus_tag="PKL42_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HNU66821.1"
                     /translation="MTQQTITSEDVKNFLQNLLLEEKKIDLKGVDLNTNLKELNIDSF
                     GFLEVIFSIEHHYNIFFPQGIDNINTLQDVIDVTYDLIQQKAKAA"
     gene            3605..4537
                     /locus_tag="PKL42_06790"
     CDS             3605..4537
                     /locus_tag="PKL42_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HNU66822.1"
                     /translation="MKIEDCGLRNNFKQLSIRYDQSHEVLWTFFNQPNMVPCANREII
                     SEIIQHQQEVENTRGVLYLNDHVYNIKYAVAASLTPNVFNLGGHLALIRGLAKNKNKE
                     ALLAYATKAIDILNSRLTRFNDCPIINISLVQGLALGAGLEGALTSDVIIAERKSLFS
                     FPEVLFNMFPGMGAHSLVMRKAGVKIADVMILGGKQYTAEQAYELGIVDVLVEDGEGE
                     QAVYKWIEKNKRSSNAYLAVQKAKNRVHPITYDELMDITRLWVDVALQLSEREFSIMD
                     RFIQMQEKLYLPNYTQHLATGTSDNIVSIQRTAG"
     gene            complement(4560..6782)
                     /locus_tag="PKL42_06795"
     CDS             complement(4560..6782)
                     /locus_tag="PKL42_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNU66823.1"
                     /translation="MNTTSRPANKALNWLLSIQSNLNALKNFVGFNQFGDEYQQSVFR
                     ILIISLIISSIFYQYDEVATSTMLIMAWFTIFSISIIFNIRKNPAQNRKRQLFAMFND
                     VAGTSISLYVTNDIGGVFVGVYLWLIIGYGFRFGKTMLIATFLTSLAGFVISSLMSPY
                     WQTHLVGFYGLLITMIAIPLHALALLNKLKDATKKAEAANKAKSEFLSHISHEIRTPL
                     NGIVGACDLLGSANLNKDHAMLFNVMKSSSNMLLELVNNVLDLSKIESGKVINNTEDF
                     YLRNLVTSTVNLFETQASQKGVSIAYEISKETPLRVHGNMLHTKQVLINLVGNAVKFT
                     EQGSVKILVTTTDQGHSQANIKFEVIDSGTGIADDSLPHIFESFVQAKESKHKLGGSG
                     LGTTISKNLVELMHGQIGVESTLGQGSNFWFEIPFEKLSSGNSQAEGVSSEIIPFKKT
                     VGKKKKNAYRILVAEDNDTNILIISQMLSMQNHTFDIVKNGELALDKLRDETYDLMIL
                     DCNMPVMGGFEALKIYQAINIGLPQTPAIILSADATNDTRALFEGLGVAAFLTKPMQS
                     EPLAEAIENAVASAKRVKAGVIHYHEAALNKTKPNVVDEAVTEKINTQIQIQMLDVGR
                     LEALANLGKSPNFLQNLVQGFISDTEKNFVQLITHILKRDYVNINDCGHAIAGSAANI
                     GADDLAKIARTINLIKPTDNENLDALLSAAVDSYEKTKLELTHYLEQRNAGENLSKAL
                     "
     gene            complement(7120..>7991)
                     /locus_tag="PKL42_06800"
     CDS             complement(7120..>7991)
                     /locus_tag="PKL42_06800"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNU66824.1"
                     /translation="GHSTFRRYSSCGRHGETNKDIRYQMLGHGATDFINKPIDHHECL
                     IRCRNLLMLRGHQKATHNRAKTLEESVKEATKNILAREHETLLRLAKAGEFRDSETGN
                     HVIRMAKYSRFIAEALGLDEERCALIEVSAPMHDIGKIGISDGILLKTGKLTDVEFST
                     MKEHTSIGHEILKDSPSKFLALGAEIALGHHEKFDGTGYPSGLKGKEIPLEARIVAVA
                     DVFDALTSERPYKKAWTNQAAIDYITEQSGKHFDPECVAAFKAQMGKVIFVQKQFNDP
                     LEQIPLGLKKAVG"
BASE COUNT         2163 a         1591 c         1743 g         2494 t
ORIGIN      
        1 aaccaagccc gtgtggcaga taaagcaacc gtgttttcaa ccagcacgcg taattttgat
       61 aaccgcatgg gccgcgatgc gcgtgtttat ctggggtctg cagagcttgc agccgtgtgc
      121 gccaaacttg gccgtatgcc aacccatgcc gagtatttag agaccgttgg taataaacta
      181 agtaatacgg ctgaaattta caaatattta aattttgacc aaatgaccga atacaaaggg
      241 gctttcgaca aagttaagaa agtgattccg attgctgaag ttatttagaa ctattcatac
      301 agtagcgctt gcaaaggtgt gttttaaaca cacttttatg agaaatgcat gatgtctgag
      361 caaacgccac ctaaagaaac gcgtgttgaa tttgttgaaa taagcccaga agaaaccctg
      421 tttgaaaaac caaaacgcgg tagttggcac aaaatgctgc cattgctact gctattagca
      481 gtgcttgttt ggactgggct atattttgga ttagataatc gggtagtagc cagtggcgcg
      541 gtggtttatg gcattttaac cagtgctttt gcctggttag tcggggtaat cggcttaatt
      601 ccaattattg ggcctttaat cgttaaggtg ctttcgattg gcgtcatctg gttgctcaat
      661 gcaattggct atttggtttc ttatatcgct atcaagcgcg gctactcaaa agatgtactc
      721 acctatcgcg ggctcactat cgccctgatt acaggcattg tcattggcta cattattggc
      781 cattacgtct agtgaagtag ctaaaggtat taaatgttgc cgctattaga tttaacttcc
      841 tggctctacc acctgaagca tcacgttaga aaattaactg acttctttcc tgcaatcttc
      901 ttctttggtg gttttttgtg ggatgcgctc actattggcc gcaacgtagc cgcctccgat
      961 ttattgatat tttctggcta ctggttggtg gcagggctaa ttctttttat tattagtcgg
     1021 ccgagctata tattaattgg ttttcctcag ccaactactc aatctaaaag ccgttatttt
     1081 aagtggctta agggtttgtt gtctcgcggt tatgcacgtt ttcactatcc tcaactgccc
     1141 tattttttat tgcagtttat ttttggcaac ctgttaagtg cgctctttat tttgtatttt
     1201 aaaagtgcga gtcattggct agcttggcta cttgcctttt tattgggtgt catgctggtg
     1261 gccaatgaat atttagaaga cgaatataaa caattcacct tgagttgggc gttatttggc
     1321 ttttgtgcca tgttactttt taatttttgc ctgcctttca tggtgggaag catgcacgct
     1381 atttggtttt accttagcac tttgttaggg gcaggcgcag cttactggct gtataaaaag
     1441 acacccaatc atttaggtag cattgcaccc gttggagtca tcgcagcgct gctgatgctg
     1501 gcctatgcgg ctgacatgat accgccagtg ccgcttgtaa agcgtgatat tgctgtcggt
     1561 tatgcactca acaaggtgga gggtagttat caattaagtc agcaaccggc aagttggtgg
     1621 gagttttggc aaaacaccag tcatgattta caagtcatcc caggacagcg ggtgtactgc
     1681 ttttcttcag tctttgcacc aagcggatta aaagcacgtt tgtaccaccg ctggcagcat
     1741 tatgattcaa aacagggctg gagaaccatg tcacgtattg gctttacgtt gtcaggcggg
     1801 cgatacgacg gttttagagg ctacacttac aaacagcagt tacaagcggg tgagtggaag
     1861 gtgagtgtag aaacggaaaa tgaaaaaacc gtggcggttc aggaatttac gatcacagaa
     1921 gtgaagactg caccgccagt tgttatgatg aaatactaag tatgtgcttg ttaacctttt
     1981 gttctaattg gttttcttga aaattaatgt agcgcccgtg ccgccaaagg cgaatgaatt
     2041 ggacatcgca tattcaagcc gctcaacttt tcttgacaca ttcggcacgt aatctaggtc
     2101 gcatttagga tcagggtttt taaggttaat ggtcggcggt agttcgttat ggtatacagc
     2161 tagtgtagtg ataatcgctt caagtgcgcc agcagcgccc attaaatgac cgtgcatgga
     2221 tttggttgag ctgatggcta aacgttgtgc atgttcccca aacacttgct taatggcatt
     2281 ggtttcggtt tcatcgttaa atttagtacc tgtgccgtgc gcattaatat actgaatatc
     2341 tctggtttgc agaccggagt ttttcatggc ggccgccatt gtggccgctt gcccttcaac
     2401 agatggaacc gcaatatgtt ggtagtcgtt ggccgcgcca tagcccacga tttctgcata
     2461 aatcgttgca ccacgcgctt tagcatgctc gtattcttct aacactaaag tcgccgcacc
     2521 ttcgcctatc accaaacctg accggtctgc agaaaatggc ttgcatgaag cactaatgtc
     2581 aacagcgtct gcatctgcaa gggtgcgaat cgcctcccat gatttaatgc tggcgtaagc
     2641 taaaattgca tcggtgccgc ctgccagcgc tacatcaatg tcaccaagtc gaatatcctt
     2701 gtaagcttgg ccaatggcca cagcagaaga tgaacacgcg gtcgtaaacg ttaaattagg
     2761 cccagaaact tgaaaatgtg tagcaatcgc tgaagccgcg ctgttgtaca tgcacatgag
     2821 tactgtgtag ggcttcaggc gattttcacc atttttatag agtgtttgat agccatcttc
     2881 aactgacaag ataccgccca tgcctgtgcc aatgtaaacg ccagactgtt ttttcagtgc
     2941 gtcattaagc gtcaggtttg catgcttgac cgcctcaatg ctagcatgca gcgccatttg
     3001 gccattacgg tctaaaatgg cgtaatcact ttttttgcta aaataatttg cagggtcaaa
     3061 ctcagcctgt gcggtcactt tgcaattaag taactctgca aagtcgccca taaatggctt
     3121 aataccggat tttcctgcga aggcgttttc ttttaatgct tcaactgtat tgccaagtgg
     3181 cgaaaccaac ccaaagccag taatgactac gcggcgcaaa tcggccatgg ttatgctgct
     3241 ttagcttttt gttgtattag gtcataggtc acatcaatga catcttgcag ggtgttaatg
     3301 ttatcaatgc cttgcggaaa aaaaatgttg taatgatgtt caatactaaa aataacttct
     3361 aaaaatccaa aggaatctat attcaattct tttaaatttg tattaaggtc tacccctttt
     3421 aagtctattt ttttttcttc taataacaag ttttgcaaaa aattcttaac gtcttcactt
     3481 gtaattgttt gttgtgtcat gcaatgtcct aagaatcacc cttaaaggct atattgtttt
     3541 taaaaagcaa cgttataatg ccataaactc cacaaaaata tgtataaaaa attaaggttt
     3601 gctgatgaaa attgaagatt gcggcttaag gaacaatttt aaacagctgt ctatccgcta
     3661 cgatcaaagt catgaagtac tctggacttt tttcaatcaa cccaatatgg tgccttgtgc
     3721 taatcgtgaa atcattagtg aaattattca acatcaacaa gaagttgaaa atacgcgtgg
     3781 cgtgttgtac ctaaatgatc atgtttacaa catcaaatac gctgtcgcgg cttcattaac
     3841 ccctaacgtg tttaacttgg gtggtcattt agcgttaatc agagggttag caaaaaacaa
     3901 gaataaagaa gcattgttgg cttatgcaac aaaggccatt gatattttaa atagccgcct
     3961 gacacggttt aatgattgcc ccattattaa cattagcttg gtacagggtt tggccttggg
     4021 cgctggctta gaaggcgcat tgactagtga tgtaattatt gccgagcgta aaagcttatt
     4081 tagctttcct gaagtgctgt tcaatatgtt tccgggcatg ggtgcgcaca gtttggtgat
     4141 gcgaaaagcg ggtgtaaaaa ttgccgacgt aatgattctt ggtggtaagc aatatacggc
     4201 tgagcaggct tatgaattgg gaattgtaga tgtattagtg gaagacggcg aaggtgagca
     4261 ggcggtttat aagtggatag aaaagaataa gcgttcgtca aacgcatatt tagcagttca
     4321 aaaggccaaa aatcgtgttc accccatcac ttatgacgag ttaatggata ttacgcgact
     4381 ctgggtagac gttgccttgc aactctctga acgtgaattt agcatcatgg atcggtttat
     4441 tcagatgcaa gagaagctct atttgccaaa ctacacgcaa catttagcaa caggcacgtc
     4501 agataatatc gtttccattc aacgtaccgc gggctaattc acaattttgg cggcgagatt
     4561 taaagcgcct ttgatagatt ttcacctgca ttgcgttgct ctaaatagtg tgtgagttcc
     4621 aattttgttt tttcataact atcaaccgcg gccgataaaa gcgcatctaa gttttcgtta
     4681 tcggttggct taatcaaatt aatggttctg gcgattttgg ctaaatcatc tgcgccaata
     4741 ttggcggcac tgccagcaat tgcatggcca caatcgttga tattgacata atcgcgcttc
     4801 aaaatatgcg taatcaattg cacaaaattc ttttcggtat cagaaataaa gccttgcact
     4861 aaattttgca aaaagttagg gcttttaccc aaatttgcta aggcttctaa cctgcccaca
     4921 tctaacattt gtatttgaat ttgagtgttg attttttcag tcactgcttc atcaactaca
     4981 ttgggttttg ttttgtttag tgctgcttca tgatagtgaa taacacccgc ttttacacgc
     5041 ttggcactgg ctaccgcatt ttcaatggcc tctgcaagcg gctcactttg catgggttta
     5101 gtgagaaaag ctgcaacgcc caagccttca aataatgctc gggtgtcatt ggtggcatca
     5161 gcacttaaaa taatggcagg agtttgtggt aaaccaatat tgatggcttg atagatttta
     5221 agtgcttcaa agccgcccat cacgggcatg ttgcagtcta gaatcatcag gtcgtaagtt
     5281 tcatcacgta atttatcaag tgctaattca ccgtttttca caatatcaaa cgtatggttt
     5341 tgcatagata gcatttgtga aatgattaaa atattggtgt cgttatcttc tgccaccaaa
     5401 atgcggtaag catttttttt ctttttgccg acggtttttt tgaatggaat aatctcagat
     5461 gaaacacctt ctgcttgtga attaccgctg ctgagttttt caaaaggaat ctcaaaccag
     5521 aagttagaac cttgtcccaa tgtgctttca acgccaattt ggccgtgcat gagttcaacc
     5581 agattttttg aaatggtcgt ccctaagcct gagccaccta atttatgttt tgattctttg
     5641 gcttgcacaa agctttcaaa aatatgcggt aatgagtcat cagcaatgcc tgtgcctgag
     5701 tcaattactt caaatttaat gttggcttgc gaatggcctt ggtccgttgt tgtgaccaat
     5761 attttcactg aaccctgctc ggtaaattta acagcgttac ctaccaagtt aatcagtact
     5821 tgtttggtgt gaagcatgtt gccatgtacg cgcagcggtg tttctttact aatttcataa
     5881 gcaattgaaa cgcccttttg cgaggcttgt gtttcaaaca aattgaccgt actggtgaca
     5941 agatttcgta aataaaaatc ttcagtgttg ttaatcactt tgccgctctc gatttttgat
     6001 aaatcaagta cattgttgac taattccagc agcatgtttg aagaggactt catcacgtta
     6061 aataacatcg cgtgatcttt attaaggtta gctgacccta acaaatcaca agcaccgaca
     6121 atgccattga gcggggtgcg aatttcatga ctgatgtgtg ataagaactc actcttagct
     6181 ttgtttgccg cttcagcttt ttttgtggca tcttttagct tgttcagtaa tgccaacgcg
     6241 tgtaatggaa tagcaatcat ggtgattaac aagccataaa aaccaaccaa atgtgtttgc
     6301 cagtatgggc tcattaaact ggagatgaca aacccagcta gactagtcag aaacgtagca
     6361 atcagcatgg ttttgccaaa tctgaagcca taaccgatga tgagccacaa atacacacca
     6421 acaaaaacac caccaatatc gttggtaacg tagagtgaaa tagaggtgcc agcaacatcg
     6481 ttgaacattg caaaaagctg tctttttcta ttttgggcag ggttttttct aatattgaag
     6541 atgattgaaa tactaaaaat ggtaaaccac gccatgatta acatcgttga tgttgcaact
     6601 tcgtcatatt gataaaaaat agacgagatg attaagctaa taatcaatat tctaaatact
     6661 gattgttggt actcatcacc aaactggttg aatccaacaa agttttttaa agcgtttagg
     6721 ttagattgaa tactaagtag ccaatttaat gctttgtttg ctggtcgtga agtagtgttc
     6781 attttttaac atttcaacaa gttaatattt aatggtaatt aagcaagagt tatgccattg
     6841 atattgatgt taaattattc atattaaaca agtagatatg tatttttatg gagatgaaaa
     6901 aaatgaaatt agttagtttt tcgtcaggta tgacaaaaat tgactattga aaaaaacacg
     6961 gcagtttgaa tggttcggca acctcaaaac agcacaaaat ttaaccccaa cctttttcga
     7021 tgctctggaa gccttataaa taaaggcttc cagagcatta tttttttctc gggagaaaat
     7081 ggggtgtttt taatggcttt atgatgtgtg atgacagggc taaccgactg ctttttttag
     7141 ccctagtggt atttgctcta aggggtcgtt aaattgtttt tgcacaaaaa tcactttgcc
     7201 catttgtgct ttaaaggcgg ctacgcattc cgggtcaaaa tgtttgccac tttgttcagt
     7261 gatgtagtcg atagcggctt ggttggtcca ggcttttttg taggggcgct cagaggtgag
     7321 ggcatcaaat acatcggcca cggctacaat gcgtgcttct agggggattt ctttgccttt
     7381 taagccgctt gggtaacccg tgccgtcaaa tttttcgtga tggcccagcg caatttctgc
     7441 acctaatgct aggaatttag aagggctgtc ttttaaaatt tcatggccaa tactggtgtg
     7501 ttctttcatg gtgctaaatt caacatcggt aagtttgccg gttttaagta aaataccatc
     7561 agaaataccg attttgccga tatcgtgcat cggcgcggac acttcaatca gcgcacagcg
     7621 ttcttcatcc aagcctaagg cttcggcaat aaagcgtgaa tattttgcca tacgaatcac
     7681 gtggttgccg gtttcagaat ctctaaactc gcccgctttt gccaagcgta acagcgtttc
     7741 atgctcacgc gcgaggatat ttttagtggc ttctttaacg gattcttcca atgttttagc
     7801 gcgattgtgc gtggcttttt gatggcctct aagcatgagt aagttgcggc agcgaatcaa
     7861 gcattcgtgg tggtcaatcg gtttgttgat aaaatctgtg gcaccgtggc ccagcatctg
     7921 gtaacgaata tctttgttgg tttctccgtg acgaccacaa ctggaatatc ttctaaatgt
     7981 ggaatgccct t
//
LOCUS       DAOQSI010000091         7967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_8679_length_7967_cov_4.718655, whole genome shotgun sequence.
ACCESSION   DAOQSI010000091 DAOQSI010000000
VERSION     DAOQSI010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 7967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7967
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8679_length_7967_cov_4.718655"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..1648
                     /locus_tag="PKL42_06805"
     CDS             179..1648
                     /locus_tag="PKL42_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNU66825.1"
                     /translation="MLRSWSLNLVVTKSSGLVVYLQIAQQIIDEIQRGRLPPSTAMPG
                     TRELAKSINVNRKTVVLAYDELIAQGWLTTENRRGTFVSSNLPNFSTPITQHDKRAPT
                     LQPAKITPYAEKLISESDTALNNIIDFNDGIPDTRLIPFKMISMAFRHALLVSSRANK
                     LGYDSPKGMLALRNSIAVMLNLERGLNVEVDNICIVRGSQMGIFLAARLLSKPNDYIV
                     VENLSYPPAREAFKSCGANVLGVGLDEHGINVDELKKLCEKYRIRAIYVTPHHQFPTT
                     VMMTAERRLKLLMLAEQYDFAIVEDDYDHEFHFSHHPVFPLASSNHHERVIYVGSLSK
                     VLAPGLRVGYLVASKAFINQCAAEVLLIDRQGNSVTELAVAELMDSGEIKRHIRRTLK
                     IYEERRNVLTQLLQNELGQYVSFDSPNGGLAIWLRLNEGIDVNALVKKALHEKVRILP
                     APMFSELPIDINAIRLGFGSLNAAELATGIQRLKRAFNA"
     gene            1881..3206
                     /locus_tag="PKL42_06810"
     CDS             1881..3206
                     /locus_tag="PKL42_06810"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat-containing protein kinase
                     family protein"
                     /protein_id="HNU66826.1"
                     /translation="MPQSQQLLQLRAGQLSGVKRLDISCGLTAFPTEIFDLADSLEVL
                     NLSGNALSSLPNDFARLKKLKILFCSNNAFTKMPEVLGQCESLSMIGFKANKISVLPE
                     NSLPKNLQWLILTDNQLTHLPSTIGQCAHLQKLMLAGNQLSNLPNSMADCTQLELLRI
                     SANRFESLPDWLLSMPRLAWLAYAGNPFCQSLEQKKSSLQPATQIDWANLTIKKQLGE
                     GASGVIYQAGWQRSDVEAKTIAVKLFKGAVTSDGLPKNEMSACIAAGTHPNLTTIEGA
                     ICNHPDHIQGLGMALIEPNFINLAHPPSFATCTRDVYATDTTFTFNQLINIADGIASA
                     AQHLHANHILHGDLYAHNILHNAQGQCLLSDFGAASFIADDLNGQAELLQKIEVRAYA
                     CLLEELIERCESPTPAIAALKSLQQDCAQANINARPLFSNITATLKALS"
     gene            3236..4102
                     /gene="ispE"
                     /locus_tag="PKL42_06815"
     CDS             3236..4102
                     /gene="ispE"
                     /locus_tag="PKL42_06815"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNU66827.1"
                     /translation="MLGFQSFLAPAKINLFLHITAQRADGYHLLQTVFRLLDFYDTVY
                     LKPSNDGLIKRTNQIAGVSEADDLCIRAAKLLQQHSQCTMGVEIALEKRIPMGGGLGG
                     GSSDAATVLLALNRLWQLNLSRAELMTLGLKLGADVPVFIHGENAWAEGVGEMLTTIM
                     LKEAYYVVLTPDAHVSTAQIFANKQLTKNTIPKTMSDFSRACSLSTGNISADFTNNLE
                     KVVCNEYPSVLACLTWLKQFGDARMSGSGASVFIEVADEKIANAICSQKPENVRGFVA
                     KGLNQHPFSTSS"
     gene            4164..4240
                     /locus_tag="PKL42_06820"
     tRNA            4164..4240
                     /locus_tag="PKL42_06820"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4198..4200,aa:Gln,seq:ttg)
     gene            4382..5332
                     /locus_tag="PKL42_06825"
     CDS             4382..5332
                     /locus_tag="PKL42_06825"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNU66828.1"
                     /translation="MSNGNLMVFTGTANPVLAQEVATHLGIELGRAHVGTFSDGETTV
                     ELLENVRGKDVFVLQSTSHPTNDSLMEVMVMVDALRRSSAGRITAAIPYFGYSRQDRR
                     PRSARVAITAKVVANMLTGVGVNRVLTMDLHSDQIQGFFDIPVDNIYATPILLSDLLK
                     QNHENLMVVSPDVGGVVRARAAAKQLNADLAIIDKRRPKPNVAKVMNIIGEVSGRTCV
                     IMDDMVDTANTLCEAAAALKNNGALKVVAYATHPVLSGGAAERIANSELDELVVTNTI
                     PLNDDTRNCKKIRQLSAAGILAETIRRISTEDSVSSLFMD"
     gene            5427..6032
                     /locus_tag="PKL42_06830"
     CDS             5427..6032
                     /locus_tag="PKL42_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HNU66829.1"
                     /translation="MSIEINAVTRDVKGTGASRRLRRAGTVPGVVYGGGQAAIPLEIN
                     AKELFLQFRHEAFHASVLNLNLDGKKESVLLRDFQMHPVRNTIQHIDFQRVSATEKIH
                     VKVPFHFINADVAPGVKLGGGIVAHVQTEADVSCLAKDLPEFIEIDVATLEMGHSIHL
                     SQIKLPKGVEFVQLAHGDDAAVASIAKTRGGVSAAAEEPAA"
     gene            6103..6678
                     /gene="pth"
                     /locus_tag="PKL42_06835"
     CDS             6103..6678
                     /gene="pth"
                     /locus_tag="PKL42_06835"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNU66830.1"
                     /translation="MSGIRLFVGLGNPGDKYEKTRHNAGFWWIDAIAAQTNSRLTADA
                     KMFGLVGKLNPAQDKWLLKPTTFMNLSGKAVAALANYYKISPAEILVIHDELDLPAGS
                     SKLKLGGGHGGHNGLKDIHAALGTANYWRLRIGIGHPGERNEVVNYVLKPPLKDEQAA
                     IDESLFDSTKVLDLLVAGEFENAMLKLHTKK"
     gene            6727..7812
                     /gene="ychF"
                     /locus_tag="PKL42_06840"
     CDS             6727..7812
                     /gene="ychF"
                     /locus_tag="PKL42_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNU66831.1"
                     /translation="MKCGIVGLPNVGKSTLFNAITKAGIAAENYPFCTIEPNVGIVEV
                     PDPRMQPLIDIVKPQKVQPAIVEFVDIAGLVAGASKGEGLGNKFLANIRETDAIAHVV
                     RCFEDSNVIHVANKIDPLSDIEVINTELALADMETVEKTMQRENKKAKSGDKDAIALI
                     KVLEKVVPCLDQAKAVRTLNLDADELQLLKPLCLITVKPVMYLANVDEKGFENNPHLD
                     KVVALGKVENAPVVCICAKIEGEISELDEEDKLLFLSELGQEEPGLNRVIRAAYDLLG
                     LQTYFTAGVQEVRAWTVKKGATAPQAAGVIHTDFERGFIRAEVIAYDEYVKNKGEQGS
                     KEAGKMRLEGKEYIVQDGDVMHFRFNV"
BASE COUNT         2354 a         1749 c         1762 g         2102 t
ORIGIN      
        1 gtatcaagca gtaacagcaa tgtaacagta tgtaaatttt caaaaacatc cagattgaca
       61 aatttttatg gtccaataga atatccttac attaataaac tttagtggtc cagtttataa
      121 atatcatata gtccagtcat cggtatatgc tgcattataa atattgaaaa ggaatactat
      181 gctgcgctct tggagcctta acttagtcgt tacaaaatca tctgggcttg tggtctattt
      241 gcagatagct caacaaatca tagatgaaat ccaaagggga cgacttccgc catccactgc
      301 catgcctggc acaagagagt tggccaaaag cattaacgtc aatagaaaaa cggtggtcct
      361 tgcctatgat gagctgattg cccaaggttg gcttaccacg gaaaatcgaa ggggtacttt
      421 tgtctcttct aacctaccaa atttctccac gcctattacg cagcacgata aacgggctcc
      481 aacacttcaa ccagcaaaaa tcacgcctta tgccgaaaag ctgatttctg aaagtgacac
      541 tgcacttaac aacatcatcg actttaatga cggcattcca gacacgcgct taattccctt
      601 caaaatgatt tccatggcgt ttcgacatgc cttattagta tcttccagag caaataaact
      661 gggctatgac agcccaaaag gcatgctggc actgcgcaat tcgattgcgg tgatgttaaa
      721 tttggaaaga ggccttaatg tcgaagtaga taacatatgt atcgtccgtg gcagccaaat
      781 gggcatattt ttggcggcgc gcctgttaag taaaccgaac gattatattg tggtagaaaa
      841 tctgagttac ccccctgccc gcgaagcttt taaatcgtgc ggtgccaatg tacttggcgt
      901 agggctggat gagcatggca ttaatgttga tgaactcaaa aagttatgtg aaaaatatcg
      961 catacgtgca atctatgtca caccgcatca tcaatttccc actactgtga tgatgactgc
     1021 cgaacgcaga ttgaaactgt tgatgctggc agagcaatat gattttgcga ttgttgaaga
     1081 tgattatgac cacgagtttc acttttcgca ccacccggta tttccgctcg ccagctcaaa
     1141 ccatcacgag cgtgtgatct atgtgggctc gctttctaag gtgctggcgc cggggttacg
     1201 agtcggttat ctagtggcat ctaaagcgtt tattaatcag tgtgcggctg aagttttgtt
     1261 aatcgacaga caaggtaact ccgtcaccga actggcggtt gcagaactca tggactctgg
     1321 cgaaattaaa cgccacattc gcagaacttt aaaaatttat gaagaacgca gaaatgtttt
     1381 aacgcagttg ctacaaaatg agctggggca atatgtgagt tttgattcac ccaatggcgg
     1441 tttggcaatt tggcttcgtt tgaatgaagg cattgacgtg aacgcgttag taaaaaaagc
     1501 cttgcacgaa aaagtaagaa tccttccagc gccaatgttt tctgaattac cgattgatat
     1561 caatgccatt cggcttggct ttggcagcct caatgcggct gagctggcca ctggcataca
     1621 acgattgaaa cgtgccttta atgcctaggt acacgcgcat cttctgacca tattttgcag
     1681 catcacatag ccaataaaac agcagaaaaa gcctcgagaa aaactttatg ctctgcgagc
     1741 ctttatttat aaggcttcca gaggcatcaa aaaggttggg gtaaaaaaca gccctttttt
     1801 agtatcattt ttagcgcatc acatagtatg attgcggctg tttaaattac cttaattttg
     1861 tgaaattacc cgtcaaacct atgccgcaat ctcaacagct tttacaattg cgcgctgggc
     1921 agttatctgg cgtaaagcgc ttggatatct cgtgcggttt aacagcgttt ccaaccgaaa
     1981 tttttgattt ggcagattct ttagaagtgc tcaatctctc gggcaatgca ttatcgtcac
     2041 tgccaaatga ttttgctcgg cttaaaaaat taaagatttt attttgctcg aacaatgcat
     2101 tcaccaaaat gccagaggta ttggggcagt gtgaatcgct tagcatgatt ggatttaaag
     2161 ctaataaaat cagcgtatta cctgaaaatt cgcttcctaa aaatctgcaa tggctcattt
     2221 taaccgataa ccaattaacg cacttgccaa gcaccattgg ccaatgcgcc cacttgcaaa
     2281 aacttatgtt agccggcaat caactatcca acttgccgaa cagcatggcg gattgcacac
     2341 aactggaatt actgcgcatt tctgcaaacc gctttgaatc tctgcccgat tggttgcttt
     2401 ctatgccacg gctggcttgg ctggcctatg cgggcaatcc gttttgccag tcactggagc
     2461 aaaaaaaatc atcactacag cccgcaacgc aaattgactg ggcaaattta accatcaaaa
     2521 aacaacttgg cgaaggtgct tcaggcgtga tttatcaagc tggttggcag cgtagcgatg
     2581 ttgaagctaa aaccattgca gtcaaattat ttaaaggcgc tgtcaccagc gatggactac
     2641 ctaaaaatga aatgtccgct tgtattgccg ccggcaccca cccaaacctc accaccattg
     2701 aaggggctat ttgcaatcat cccgaccata ttcaagggct aggcatggca ttgattgagc
     2761 caaattttat taatcttgct cacccgccca gctttgccac ttgcacgcga gatgtttatg
     2821 ccactgacac gacctttacc tttaaccagc tcatcaatat tgccgatggc attgccagtg
     2881 ctgcacaaca tttacacgct aaccacattt tacatggcga tttatacgcg cacaatattt
     2941 tgcataatgc gcaaggccaa tgcttgttaa gcgattttgg cgccgcttca tttattgctg
     3001 atgacttaaa tgggcaagcc gaattgttac aaaaaatcga agtacgtgcc tatgcctgct
     3061 tgcttgaaga actgattgag cgatgcgagt cgccaacgcc agcaattgca gcgttaaagt
     3121 cactgcaaca agactgcgcg caagccaata tcaacgctag gccattattt tccaatataa
     3181 ccgctaccct caaagcgtta tcataaatgc ctcacatgcg ttaaaatgca aggctatgct
     3241 aggttttcaa tcatttttag caccggcaaa aatcaacctg tttttgcaca tcactgccca
     3301 acgtgcggac ggctatcatc tacttcaaac cgtttttcgt ttgttggatt tttatgacac
     3361 cgtttattta aaacctagca acgacggact tatcaaacgt acaaatcaaa tcgctggtgt
     3421 gtcagaagcc gatgatttat gcatacgcgc cgccaaatta ttacagcagc acagccaatg
     3481 caccatgggc gtagaaatcg cacttgaaaa acgcattcca atgggtggcg ggcttggtgg
     3541 cggaagttct gatgcggcaa ccgtgctgtt agctctcaac cgcctgtggc agctcaattt
     3601 aagccgcgca gaactcatga cccttgggct aaaactaggt gccgatgtgc ctgtttttat
     3661 tcatggtgaa aacgcctggg cagaaggcgt gggcgaaatg ctgacaacca tcatgctaaa
     3721 agaggcttat tacgttgtat taacgcccga tgcgcatgta tcaaccgcgc aaatatttgc
     3781 caataagcaa ttgacaaaaa acacgattcc taagacaatg tcggactttt caagggcatg
     3841 cagtctcagc acaggaaata tcagcgctga ttttactaat aatcttgaaa aagtagtttg
     3901 caatgaatac ccctctgtat tggcatgcct cacatggctt aagcaatttg gtgatgcgcg
     3961 catgagcggt tctggtgctt ccgtgtttat agaagtagcg gatgaaaaaa ttgcaaatgc
     4021 aatatgcagt caaaagcccg aaaatgtgcg ggggtttgtt gcaaaagggc tcaatcagca
     4081 cccttttagc acttctagct aacttgttag cgtagaaagc actgatcagt ttattctgat
     4141 aaaaaataaa gccgattaaa tattggggag tcgccaagct ggttaaggca ccggattttg
     4201 attccggcat gcgaaggttc gaatccttcc tccccagcca gaatttaaaa tgcagacaat
     4261 ccgctgcatg aaatagaaca acaagcgtgt agatttctga tttacgcgct tttattttta
     4321 gtaagctttt attgatggtt acacgctggc aacacacgcc tgcacaatag gaaatcaact
     4381 gatgtctaac ggcaacttga tggtctttac tggcactgcc aacccagtgc ttgcacaaga
     4441 agtggctacc caccttggca ttgagctagg ccgtgcgcat gtaggcactt tttcagatgg
     4501 tgaaactacg gtagaactgc ttgaaaatgt acgtggtaaa gacgtgtttg tattgcagtc
     4561 aaccagccac ccaacaaacg acagcctgat ggaagtgatg gtgatggtag atgcgctaag
     4621 gcgctcatcc gctgggcgca ttacggcggc aatcccctac tttggttatt ccagacaaga
     4681 tcgccgtcca cgttctgcac gtgttgccat taccgcaaaa gtagtagcga acatgctgac
     4741 aggcgttggc gtcaaccgtg tgctgaccat ggatttgcac tcagaccaaa ttcaaggttt
     4801 ttttgatatt cctgtagata acatttatgc aacacccatt ttgttgtcag atctattaaa
     4861 acaaaatcat gaaaacttaa tggtggtgtc gcccgatgtt ggcggtgtgg ttcgtgcccg
     4921 tgctgcagca aaacaattaa acgctgactt ggctattatt gacaagcgtc gccctaaacc
     4981 aaatgttgct aaagtgatga acatcattgg tgaagtttca gggcgtacct gtgtgattat
     5041 ggatgacatg gtcgataccg ccaatacatt atgtgaagcc gccgccgcac ttaaaaataa
     5101 cggcgccctt aaggtagtgg cctatgccac gcacccagtg ttgtcaggcg gtgcagcaga
     5161 acgtattgct aactctgaat tagatgaatt ggtagtgacc aatacgattc cactcaatga
     5221 cgatacaaga aactgtaaaa aaattcgcca attaagtgcc gctggcattt tggctgaaac
     5281 gatacgcaga attagtactg aagactccgt ttcttcacta tttatggatt aactaaactg
     5341 tttttaaccc tgttttctgg tcgcggaaaa cagatttttg tgtagtgccc gctgcgtaaa
     5401 atacagtggt tttaggagta gtaaaaatgt ctatcgaaat taatgcagta acgcgtgatg
     5461 tcaaaggtac gggtgcgagc cgccgtctac gtcgcgctgg cacagtgcca ggtgtggttt
     5521 atggtggcgg tcaagcagcc attccacttg aaatcaatgc gaaagaattg ttcttacaat
     5581 tccgtcacga agcttttcat gcttcagtgt taaacctaaa cctagatggc aaaaaagaaa
     5641 gcgtattgct acgcgacttt caaatgcatc cagtccgcaa cactattcaa cacattgact
     5701 ttcaacgtgt aagtgctact gaaaaaatcc acgtaaaagt acctttccac ttcatcaatg
     5761 cagatgttgc acctggtgtt aaattaggtg gcggcattgt ggcgcacgta caaacagaag
     5821 ccgatgtaag ctgcttggca aaagacttgc cagagtttat tgaaattgac gttgccacac
     5881 ttgaaatggg tcactcaatt cacttatcac aaattaaact accaaaaggt gttgaatttg
     5941 tgcaattagc gcatggtgat gacgcagcgg ttgcctcaat tgccaaaacg cgcggcggtg
     6001 tttctgcagc tgcagaagaa ccagcagctt aagtttactg acatgcaaat taaacgcgca
     6061 ctttgctatg aagtgcgcgt tttttattga tactattgac ctatgagtgg aattagactt
     6121 tttgtgggat taggcaaccc aggcgacaag tacgaaaaaa cacgccataa cgcaggcttt
     6181 tggtggatag atgcaattgc agcgcaaacc aatagcagat taacggctga tgcaaaaatg
     6241 tttggattag ttggtaagct aaatccagcg caagataaat ggctactcaa accgactact
     6301 tttatgaacc tcagcggcaa agccgttgca gcactggcga actattataa aatttctcct
     6361 gctgaaattt tagtgattca tgatgagctt gatttgccag caggtagcag caagttaaaa
     6421 ttaggtggcg gccacggtgg tcacaatggc ttaaaagata ttcacgcagc cttaggaaca
     6481 gcaaactatt ggcgtttacg cattggcatt ggtcaccctg gtgagcgcaa tgaagtggtt
     6541 aactatgtac tgaaaccacc attaaaagat gagcaagctg ctattgatga aagccttttt
     6601 gatagcacta aagtgctaga cttactggtg gcaggtgagt ttgaaaatgc aatgttgaaa
     6661 ttgcacacga agaaatgact gaaatagaaa taacctaata aagtacctaa tagagaaagt
     6721 attgatatga agtgtggcat cgtaggtcta ccaaacgtag gaaaatcaac gttattcaac
     6781 gccattacca aagcaggtat tgcggctgag aactatcctt tttgcacgat tgaacccaac
     6841 gtcggcattg tggaagtacc agacccccgc atgcaaccgc tcattgacat cgtaaaacca
     6901 caaaaggtgc aacctgccat tgtagagttt gtcgatatcg caggcttagt agcgggcgct
     6961 tcaaaaggtg aaggtttagg taacaaattc ttagccaaca tccgcgaaac agacgccatt
     7021 gcccatgtag tgcgttgctt tgaagacagc aatgttattc acgttgccaa taaaattgac
     7081 ccactttctg atattgaagt catcaataca gagcttgccc ttgccgacat ggaaaccgtt
     7141 gaaaaaacca tgcagcgtga aaacaaaaaa gccaaaagcg gcgacaagga tgcgattgcg
     7201 ctcattaaag tattagaaaa agttgtaccc tgcttagacc aagccaaagc cgtacgaaca
     7261 cttaatttgg atgcagatga gttacagcta ctcaaaccac tttgccttat caccgttaaa
     7321 cccgtgatgt acttagccaa cgtcgatgaa aaaggctttg aaaacaaccc gcacctcgat
     7381 aaagttgttg ccttaggcaa agttgaaaac gcccctgtgg tatgtatatg cgccaaaatt
     7441 gaaggtgaaa tatcagagtt agacgaagaa gataaactgt tatttttatc agaactcggc
     7501 caagaagagc caggcctaaa ccgcgtgatt cgtgccgctt atgacttact tggcctgcaa
     7561 acttacttca ccgcaggtgt gcaagaagta cgcgcctgga ctgtaaaaaa aggcgcaacc
     7621 gcaccccaag cagcaggcgt tatccacaca gactttgaac gtggctttat tcgtgcagaa
     7681 gtcattgcat atgacgaata cgttaaaaac aaaggcgagc aaggcagcaa agaagctgga
     7741 aaaatgcgct tagaaggcaa agaatatatt gtgcaagatg gtgatgtaat gcactttaga
     7801 tttaatgttt aacaacgcta gactgaataa gaaaacgcaa actatttttg acaattggcg
     7861 actaaaacac tagaattgcg cccttgcttt agtttaggca tcaagtttac agggtgatgt
     7921 agctcagctg gttagagcac aggattcata atcctggggt cgagggt
//
LOCUS       DAOQSI010000092         7904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_8778_length_7904_cov_4.660339, whole genome shotgun sequence.
ACCESSION   DAOQSI010000092 DAOQSI010000000
VERSION     DAOQSI010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 7904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7904
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8778_length_7904_cov_4.660339"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PKL42_06845"
     CDS             complement(<1..466)
                     /locus_tag="PKL42_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HNU66832.1"
                     /translation="MSFKHITSRDNPIFKFYKKLSENARERRTEGKTLLDGVHLIEAY
                     MAAFGEPEVLIIPEGKSSVEATRLIQSLEHIATVMLPTLMFAEITPVATSTGILALVN
                     MPKLGSPDAPGFALLLEDIQDPGNLGSMLRTAAAAGVEVVYLSEACTDAWSPK"
     gene            606..1292
                     /gene="aqpZ"
                     /locus_tag="PKL42_06850"
     CDS             606..1292
                     /gene="aqpZ"
                     /locus_tag="PKL42_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HNU66833.1"
                     /translation="MSLTKRSLAELIGTFWLVLGGCGSAVLAAGIPDLGLGYLGVSFA
                     FGLTVVTMAYAIGHISGCHLNPAVSIGLVAGGRFKAAELPHYIVAQVIGAILAALLIK
                     TIASGAEGFAGGLASNGFAEHSPHGYSMVAGLITEIVMTAMFLFIIMGATDKRAPAGL
                     APIAIGFTLVLIHLISIPVTNTSVNPARSTGPALLEGGIALQQLWLFWIAPIVGAVIG
                     AVAYKTVSEE"
     gene            1386..2102
                     /locus_tag="PKL42_06855"
     CDS             1386..2102
                     /locus_tag="PKL42_06855"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HNU66834.1"
                     /translation="MAKKLDLERILKNQGFGARKLCRIMIINEEITVNGEVCDDPSAE
                     FDLENLHFTVKGEPWQYRENSYLMMHKPGNYECSHKTQHHPTIYSLLPHPLVERGVQC
                     IGRLDEDTTGLILISDDGQFIHRMSSPKHKVPKVYEVTCKHPVSDEQIAHILQGVQLV
                     DEDAPIAALACERITENIIHMTLAEGKYHQVKRMVAAISNRVEGLKRIQIGELKLPED
                     LKVGEWRWLTTDDMLKLNAN"
     gene            2105..2557
                     /locus_tag="PKL42_06860"
     CDS             2105..2557
                     /locus_tag="PKL42_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66835.1"
                     /translation="MDIVKLTKFEVAKIQLVKAIELFCSQEPLPAITLAGAAEEILGK
                     LVKKGGGTNALEEEIKDQCELYQTVFGCSGEPREFATLMNNSRNELKHLMSGNQLEFN
                     LEEEAVNLFQRAIDNFQKLEPGPNTVFQQFDDKAAAWYRRYSSRRAEK"
     gene            2746..4083
                     /gene="chrA"
                     /locus_tag="PKL42_06865"
     CDS             2746..4083
                     /gene="chrA"
                     /locus_tag="PKL42_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HNU66836.1"
                     /translation="MTDLKHNIAPPTPSLTEAFWYWLKLGFISFGGPAGQIAIMHQDL
                     VEKKRWISEKRFLHALNYCMVLPGPEAQQLATYIGWLMHKTWGGIIAGGLFVLPSLLI
                     LIALSWGYMRFGDLPQVAAMLYGIKPAVVAIVLFAAYRIGKRILNNAMLWGIAIAAFM
                     AISVLHLPFPAIIVAAALFGHVAHRYYPARFNVNNAHAAASKRFGAAVIDDTTPTPAH
                     AQFSWPTLWRRLAIGLGIWLVAMAMLILTQGWQTALTQMAWFFTKAAMLTFGGAYAVL
                     PYVYQGAVEHYQWLTPHQMMDGLALGETTPGPLIMVVAFVGFVGGWSQTVLGADNLVL
                     SGILAATVVTFFTFLPSFLFIFIGAPLIESSQNNLKLTAPLNAISAAVVGVVVSLAVF
                     FAQHIFVPDATFKVDALAILITLFSLVALVKWNLSIIKLVLLCGIIGVVNYFF"
     gene            4101..4667
                     /locus_tag="PKL42_06870"
     CDS             4101..4667
                     /locus_tag="PKL42_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HNU66837.1"
                     /translation="MKRLILLLALSLSACMGEMPKGVQAVDNVDAQQYLGTWYEIARL
                     DHSFERDLEQVTANYSLRDDGGIKVINRGYNIKNKTWKEAEGKAYFVEPANKDGTYSG
                     KLKVSFFGPFYGPYHIIALDKTMYNYALVTSGKEYLWILSRTPQLTYPIKQELMSQAK
                     ALGYPTENLIFVKQANQVEYESGRHVVH"
     gene            complement(4776..6917)
                     /locus_tag="PKL42_06875"
     CDS             complement(4776..6917)
                     /locus_tag="PKL42_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNU66838.1"
                     /translation="MLAKPINSYRPYWAKRFGTAKMLPMTRAEMDAQGWDSCDIILIS
                     GDAYIDHPSFGVSLVGRLLEAQGFRVGIIAQPDWQNASAFKALGKPNLYFGVTAGNMD
                     SMVNRYTADRKIRSDDAYTPDAAPNKRPDRAVLVYSQRCREAYSDVPLVIGSIEASLR
                     RIAHYDYWSDKVRRSILVDSKADILLYGNAERALVELSHRIAKGEKVSDIQDIRGTAF
                     LRKAIPDGWSEIESTKLDRPGAVEKHVDPYEMKLGKSDASCATDNPSPAGEGNVTHVI
                     AMPLSRKPKADRSKQVVRLPAYEEVASNPVMYAHASRVLHLEANPGNARALVQRHGDR
                     EVWLNPPPIPLTTKEMDYVYDLPYARAPHPIYKQAKIPAWEMIRFSVNIMRGCFGGCT
                     FCSITEHEGRIIQSRSEASILKEVEEIRDKVDGFTGVISDLGGPTANMYRIACKSEEI
                     EKNCRKLSCVYPSVCDNLNTDHSALIQLYRKARAIKGVKKILIGSGVRYDLAVKSPEY
                     VKELVQHHVGGYLKIAPEHSEENVLSKMMKPGMDAYDEFKAMFEKFSAEAGKKQYLIP
                     YFIAAHPGTTDQDMLNLALWLKKYDFKLDQVQTFTPTPMAMATAMYHSGKNPLRKVTA
                     DSENVPIPKAGNVRKLHKAFIRYHDPNNWPMLREALKKMGREDLIGSGKHCLIPAWQP
                     LSNKPITTVRGEQLERSVRPKRLHENKRKYS"
     gene            complement(7044..7394)
                     /locus_tag="PKL42_06880"
     CDS             complement(7044..7394)
                     /locus_tag="PKL42_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004644745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HNU66839.1"
                     /translation="MQYIILKYLITAGVVVLVSELAKRSDKLGSLVAALPLVTVLTLI
                     WLYVEQQPTSKIANHAYYTFWYVLPTLPMFLLFPYLLPKIGFVYTLISCVVFTMLVFW
                     GFALMMRLFGVDLL"
     gene            complement(7410..>7904)
                     /locus_tag="PKL42_06885"
     CDS             complement(7410..>7904)
                     /locus_tag="PKL42_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HNU66840.1"
                     /translation="MRAIEFVASELGDWALHCHKSHHTMNAMGHGVPTLLGVRQDDLV
                     EKISNLVPDYMPMGETGMAEMTDMANMMDMPLPENTLPMMMGKGQYGDISMGGMFSTL
                     KVRDHLAKSDYQDPGPYQHPKGTVAYAFNGDVPEAKRQPVVDTKTAVEVQVRKPSGHA
                     GHSH"
BASE COUNT         2069 a         1859 c         1798 g         2178 t
ORIGIN      
        1 ctttaggtga ccaggcatcg gtacaggctt ctgacaaata caccacttca acacccgctg
       61 ctgctgcggt gcgcaacata ctgcccaaat tgcccgggtc ttgaatgtct tctagcaata
      121 aagcaaaacc gggggcatcg gggctgccta acttaggcat attcaccagc gccaaaatcc
      181 cggtactggt tgccactggc gtaatttctg caaacattag cgtgggtagc atcacggttg
      241 caatatgttc gagcgattga attaagcgcg tagcttcaac actacttttg ccctccggaa
      301 taattaatac ttctggctca ccaaaagccg ccatgtaagc ttcaatcaaa tgcacgccat
      361 cgagcaaagt tttgccctca gtgcggcgct cacgcgcatt ttccgaaagc tttttataaa
      421 atttgaatat tgggttatcg cgtgaggtaa tgtgcttaaa actcataaaa tcctaatcaa
      481 ctaaagattg tttacaaaat ctagcgatag tttaacccaa acaaggttgt atgcatgcca
      541 gctttacagt aaattggcag ggcagtaacc attcaacaaa aaactatttc aatacggagg
      601 gtgagatgtc attaacaaaa cgttcattag cagaattaat tggtacattt tggttagtat
      661 taggcggttg cggtagcgct gtgctggctg caggtatccc tgatttaggc ttagggtatt
      721 taggtgtttc atttgcgttt ggcttaacgg ttgtgaccat ggcttatgcc attggccata
      781 tttcgggttg ccatttaaac cctgcagtat caattggttt ggtggcaggc ggccgattta
      841 aagctgccga attaccacat tacattgtgg cgcaagtcat tggcgcaatt ttggcagcct
      901 tgttaattaa aaccattgcc agtggcgcgg aaggctttgc aggcggtttg gcttctaacg
      961 gttttgcaga acactctccg catggctaca gcatggtggc tggtttaatc acagaaatcg
     1021 tgatgacagc gatgttcttg tttatcatca tgggcgctac cgacaaacgc gcacctgcag
     1081 gattagcacc gattgcaatt ggcttcacct tggtgcttat tcatttaatt agcattcctg
     1141 taaccaacac ctcagtcaat cctgcgcgta gtactggtcc agcgttactt gaaggcggca
     1201 ttgccttgca acagctttgg ttgttttgga tagcacctat cgttggcgca gtgattggcg
     1261 cggtggctta taaaaccgtt agcgaagaat aaggcctatt gtaggagcgc agtttattgc
     1321 gcccctacaa aaaactaagc gcccacatca cgtgggcgtt tttatttcta acgctataat
     1381 agcgcatggc taaaaaactc gatttagaac gcatcttaaa aaaccaaggc tttggcgcac
     1441 gtaagctttg tcgcattatg attatcaatg aagaaatcac cgtcaatggt gaagtttgcg
     1501 atgacccaag tgctgaattt gaccttgaaa acttgcattt cacggtaaaa ggtgagcctt
     1561 ggcaataccg tgaaaactcc tacctgatga tgcacaaacc gggcaattac gagtgttcac
     1621 acaaaaccca gcaccatcct actatttaca gcttactacc acacccattg gtagaaagag
     1681 gcgtgcaatg tatcggcagg cttgatgaag acaccacggg cctgatttta atttcagacg
     1741 acgggcaatt tatccaccgc atgagttcgc ccaaacataa agtgccaaaa gtgtacgaag
     1801 taacctgcaa acatcctgta agtgatgagc aaatcgcgca cattttacaa ggcgtgcagt
     1861 tggtggatga agatgcgccg attgccgcgc tggcctgtga gcgcatcact gaaaatatta
     1921 tccatatgac cttagctgag ggcaaatatc accaagtcaa acgtatggtg gcggccatca
     1981 gtaatcgtgt agagggttta aaacgcatcc aaattggtga actgaagcta cctgaagatt
     2041 taaaagtggg tgaatggcgg tggcttacaa cagatgatat gctaaaacta aatgcaaatt
     2101 gaatatggat atcgtgaaac tgactaagtt tgaggttgcg aaaatacaac tcgtaaaagc
     2161 catcgagttg ttttgttcgc aagagccgct gccagctatc acattggcag gtgctgcgga
     2221 agagatactt gggaaactcg ttaagaaagg tgggggtaca aatgctcttg aggaggaaat
     2281 aaaggatcag tgtgaactgt atcagacagt gtttggctgc tcaggcgagc ctagggagtt
     2341 tgccactctg atgaataact caagaaacga acttaaacac ctaatgtctg gaaatcaatt
     2401 ggagtttaat cttgaggaag aagccgtcaa cttgtttcag cgggctatcg acaactttca
     2461 aaaacttgaa ccaggtccta atacagtatt tcagcaattt gatgataagg cagcagcttg
     2521 gtaccgtaga tacagcagtc gtagggcgga aaagtgaaac gcattctgcc gtatgtcatc
     2581 tttcttacgg cggataacgc tgtgctcatc cgccctacat ctgacacttt tttgtaatca
     2641 ttttatgcta aattttacac atctttacct aaacacctct acaagccagt ctccatgcgg
     2701 cttgtagggc agtgtttttc tctcggggat ttttaagggt catttttgac tgatttaaag
     2761 cacaatattg cacccccaac acccagccta accgaagctt tttggtattg gcttaaactc
     2821 ggctttatca gctttggtgg gcctgctggt caaatcgcga ttatgcatca agatttggtt
     2881 gagaaaaaac gctggatttc agaaaagcgc tttttacatg cgttgaatta ctgcatggta
     2941 ttacctggtc cagaagcaca acaattagcc acctacatcg gctggctcat gcacaaaacg
     3001 tggggcggca tcattgcggg cggcttattt gtattgcctt cactacttat tttaatcgcg
     3061 ctgagttggg gctatatgcg ctttggcgat ttaccgcaag ttgccgccat gttgtacggc
     3121 atcaagccgg cggtggtggc catcgtgtta tttgcagcat accgcatagg caaacgcatt
     3181 cttaataacg caatgttatg gggtattgcc attgctgctt ttatggcgat tagtgtgttg
     3241 catttgcctt ttcctgccat tattgttgcg gcagccttgt ttggccatgt tgcacatcgc
     3301 tactaccccg cacggtttaa tgttaacaac gcacatgctg cagcgagcaa aagatttggc
     3361 gcggctgtta ttgatgatac aacgcccacc cccgcgcacg ctcaatttag ctggcccacc
     3421 ctttggcgca gattagcgat tggtttgggc atatggctag tagcaatggc catgttgata
     3481 ctcacgcaag gttggcaaac cgcactcacg cagatggcct ggttttttac caaagccgcc
     3541 atgctcactt ttggcggcgc gtatgcggta ttgccgtatg tttaccaggg cgcagttgaa
     3601 cattaccaat ggctaacacc acatcaaatg atggatggcc tagcgcttgg tgaaaccaca
     3661 cccggcccgt tgattatggt ggtggcattt gttggatttg ttggcggctg gtcacaaacc
     3721 gtgttaggcg cagataattt agtgttatcc ggcattttgg cggcaactgt agtgacattt
     3781 ttcacctttt taccttcttt tctgttcatt tttattggcg cgcccctgat tgagtcaagc
     3841 caaaataatc tcaaactcac cgccccgctc aatgccattt ctgcggccgt cgtcggcgtg
     3901 gttgtgagtt tagcggtatt ttttgcacag cacatttttg tgcctgatgc cacatttaaa
     3961 gtagatgcat tggccatttt gattacttta ttttcactcg tagcactggt gaaatggaac
     4021 ttgagtatca tcaaactagt cttgctttgt ggcatcattg gcgtcgttaa ttactttttt
     4081 taaatataga aaccaatctt atgaaacgtt taattctgct gttagcgcta agtttaagtg
     4141 cgtgtatggg tgaaatgcct aaaggtgtgc aagccgtgga caacgtagat gcccagcaat
     4201 acttaggcac atggtatgaa atcgcccggc tcgaccacag cttcgagcgc gatttagaac
     4261 aagtcaccgc caattacagc ctgcgtgacg acggcggcat taaagtgatt aaccgcggct
     4321 acaacatcaa aaataaaaca tggaaagaag cagaaggcaa agcgtatttt gttgaacccg
     4381 cgaacaaaga tggcacttat agtggcaaat tgaaagtctc tttttttggc ccgttttatg
     4441 ggccgtacca catcatcgcg ctagataaaa ccatgtacaa ctacgcctta gtgaccagcg
     4501 gaaaagagta tttatggata ctttcgcgca caccgcaact cacctacccg attaaacaag
     4561 agctgatgtc gcaagccaaa gcattgggtt acccgactga aaatctgatt tttgtaaaac
     4621 aagctaacca agtggaatat gagtctggcc ggcatgtggt tcactagtta tatacacgca
     4681 tcttcttgac cgctgccctg caagccttat aaaacaaggc ttgcagggca gcaaaaatct
     4741 ctcggggcca aaaacaggtt tttatagtaa aaaacttaag agtactttcg cttgttttca
     4801 tgtaaacgtt ttggccgcac actgcgctcg agttgttcgc cgcgcacggt ggtaatcggt
     4861 ttatttgaga gcggttgcca agcgggaatc aggcaatgtt tgccgctgcc aatcaaatct
     4921 tctcgaccca ttttcttgag tgcctcacgt aacattggcc aattattggg gtcgtgatag
     4981 cgaataaacg ctttgtgcag tttgcgcacg ttgcctgctt ttggaattgg cacgttttca
     5041 gaatctgccg tcactttacg cagtggattt ttacctgaat gatacatggc ggttgccatg
     5101 gccatcggtg tgggggtaaa tgtttgcact tggtcgagct tgaaatcgta ttttttcagc
     5161 caaagtgcca aattgagcat gtcttggtct gtggtgccag ggtgcgcggc aataaagtac
     5221 ggtatcaaat attgcttttt accggcctct gcgctgaatt tttcaaacat cgctttaaat
     5281 tcatcataag cgtccatgcc cggcttcatc attttgctta agacattttc ctcagaatgc
     5341 tctggcgcaa ttttgaggta gccgcctaca tgatgctgca ctagttcttt tacgtattca
     5401 ggtgatttca cggccaaatc gtaacgcaca ccgctgccga ttaaaatttt cttcacaccc
     5461 tttatcgcac gcgctttgcg ataaagctga ataagcgcac tgtggtcggt gtttaaatta
     5521 tcacaaacgc tgggataaac gcagcttaat ttgcggcaat ttttctcaat ttcctcactc
     5581 ttacaggcaa tgcggtacat attggccgtt gggccgccta ggtctgaaat tacaccggta
     5641 aagccatcga ctttgtcgcg aatttcttcg acttctttaa gaatgctcgc ttcactacgg
     5701 ctttgaataa tacggccttc atgctcggta atactgcaaa aggtgcaacc gccaaagcag
     5761 ccgcgcatga tattgactga aaagcgaatc atctcccaag cgggaatttt tgcttgtttg
     5821 taaatgggat ggggtgcacg cgcgtatggc aagtcgtaca cataatccat ctcttttgta
     5881 gtcagcggaa ttggcggtgg attcagccac acttcgcggt caccatgcct ttgcactaag
     5941 gcgcgggcat tgccggggtt tgcttctaaa tgcaatacgc gcgaggcatg tgcgtacatc
     6001 actggattgc ttgccacctc ttcataagcg ggcaagcgca ccacttgttt gctacggtcg
     6061 gccttcggct tacgtgatag tggcatggca atgacatgcg ttacattccc ctcgcctgcg
     6121 ggcgagggat tgtcagttgc gcaactggca tctgatttac ccaatttcat ttcatacggg
     6181 tcaacgtgtt tttccaccgc accggggcgg tctaacttgg tactctcaat ctcgctccag
     6241 ccatcgggga tcgcttttct caaaaaagca gtgccgcgta tatcttgaat gtcactgact
     6301 ttttcacctt ttgcaatgcg gtggctgagt tcaaccagtg cacgctcggc gttgccatac
     6361 agcaaaatat ccgctttaga atccaccaaa atactacggc gtactttgtc agaccaataa
     6421 tcataatgcg caatgcggcg caagctagct tcaatgctgc ctattaccaa cggcacatca
     6481 gaatacgctt cgcggcagcg ctgcgaatac actagcaccg cacggtcagg gcgtttgtta
     6541 ggcgcggcat ctggtgtgta ggcgtcatct gaacggattt ttcggtcagc cgtatagcga
     6601 ttgaccatgc tatccatatt gcccgcagtc acaccaaagt acagattcgg cttgcctagc
     6661 gctttaaaag cgctagcatt ttgccaatct ggctgtgcaa taatccccac acgaaaaccc
     6721 tgcgcttcaa gcaaacgccc caccaatgac acaccaaaac tcgggtggtc aatatacgca
     6781 tcgcccgaaa ttaaaataat atcgcagcta tcccaacctt gcgcatccat ctcagcgcgc
     6841 gtcatcggca gcattttggc cgtgccaaac cgtttcgccc aataagggcg ataggaatta
     6901 atcggcttag ccagcatggt ggtatagtgt tccaaaattt ctacacaatc aataaaagtg
     6961 cgcgcatttt acgcgctaat ggtttgatta aaaagctatt tttaaagaaa aattcacttc
     7021 aataccccta taaaatttag gctttaaagt aaatccaccc caaacagacg catcatcaat
     7081 gcaaaacccc aaaaaaccag catggtgaat acaacacagc tgattaacgt atagacaaag
     7141 ccaatctttg gtaacaaata cggaaacagc aaaaacatcg gtaaagtcgg caacacatac
     7201 caaaatgtgt aataggcatg atttgctatt ttactggtcg gttgctgctc aacgtacagc
     7261 caaataagtg tgagtacggt cactaaagga agtgcagcca ctaagctacc cagcttatcg
     7321 ctgcgtttgg ccaattcaga taccagcacc acaacaccgg cggtaatgag atatttcaag
     7381 ataatatatt gcatcttaac tccttaactt tagtgagaat gtccggcgtg gccgctcggt
     7441 ttacgcacct gaacttctac agccgttttt gtatcaacta ctggctgtct ttttgcttca
     7501 ggcacatcac cgttaaacgc ataggcaacg gtccctttcg gatgttgata tggccctgga
     7561 tcctgataat cactttttgc cagatgatca cgcactttca gcgtagaaaa catgccgccc
     7621 atactgatat caccatactg ccccttaccc atcatcatgg gcagtgtatt ttcaggtagc
     7681 ggcatgtcca tcatattggc catgtcggtc atttccgcca tgcctgtttc acccattggc
     7741 atataatctg gtacaagatt actaattttc tcaaccaaat catcttgcct tacaccaagt
     7801 aaagtgggta ctccatgccc catcgcgttc atggtgtggt ggcttttatg gcaatgtagc
     7861 gcccaatcgc ctaattcact cgccacaaac tcaatagcgc gcat
//
LOCUS       DAOQSI010000093         7888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_8808_length_7888_cov_5.219328, whole genome shotgun sequence.
ACCESSION   DAOQSI010000093 DAOQSI010000000
VERSION     DAOQSI010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 7888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7888
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8808_length_7888_cov_5.219328"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="PKL42_06890"
     CDS             complement(<1..1161)
                     /locus_tag="PKL42_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66841.1"
                     /translation="MQKQKNPILWKRGALWLAFFGPFFFLSYGWLNQFTSTRTDVGVA
                     VAAWEAAIPFVPWMMLPYMSIDAFYAGSLFLFRKRHALDRHALRLLLATTISLIGFLL
                     FPLQFSFAVPKIDGFNGFLQTILLGFDKPYNQAPSLHISLLIVLWVVYAKRLQGFWRL
                     ALHTWLFAIGASVLLVYQHHFIDVWTGALAGVACLYFIPDRPFYWRKKLPNHRMILMA
                     WRYVMGAVVALMAGLYLKQLSSFFTLLFSWVAIALALVAAAYFGFEKQVFQRHQGVMR
                     WPARLMLAPYLLGSWLSYRVYTRNNRHPVEIKNGVWLGAFPDKNMFQTRWLAVLDLTN
                     EFTTTPFKNVAHHKFLPVMDLTPPAPIVLVRAVRWMTFVGKQANLQDDAHAIL"
     gene            complement(1393..3183)
                     /locus_tag="PKL42_06895"
     CDS             complement(1393..3183)
                     /locus_tag="PKL42_06895"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HNU66842.1"
                     /translation="MGRVALERTFTAVDNAEIFYRHWPSEGQASKVNDTKRAVILFHR
                     GHEHSGRQQEVVDSLNMPDYDCFAWDARGLGRSPGVRGYAENFGVFVKDADDFAHHIC
                     RQYGFKLENIAVIAQSVGGVIAAAWVHDYAPNIRCLVLASPALKVKLYVPFARTALGL
                     LQKLGLTKLVPALAFVNSYVKAKWLTHDEARIASYERDHLITRPIAVNILLELYETAE
                     RLVKDAAAITTPTQFLVSGSDWVVHQTPVRDLYQNLGAKTKEYHVFEGFFHDTLGEKD
                     RHLPLAKAREFIAAQFATPFASQDLINADQAGYTFDEAEQLKKPLPTFSLKNLYFAAT
                     RANIRFGSQLSRGLRLGVDTGFDSGSTLDYVYENQARSTTFIGGALGEIIDRTYLNAI
                     GWRGIRVRKQHIEALLAKAAHLLRENAKEVRIMDIAAGHGRYALEAAVAVKADHVLLR
                     DYSDLNVAGGRELIQKLGLASKAQFEKADAFDEKSIATVTPKPTIAIVSGLYELFPEN
                     APIGASLAGVHQALEAGGYLIYTNQPWHPQLELIARTLTSHRQNKAWIMRRRTQREMD
                     QLVANAGFEKIDQWSDTWGIFSVSLAKKLD"
     gene            complement(3306..3983)
                     /locus_tag="PKL42_06900"
     CDS             complement(3306..3983)
                     /locus_tag="PKL42_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66843.1"
                     /translation="MLIRALAIFLMLFTVNLLADEGENPSEANIQQISSQDGAVQDAA
                     NQAIHQELRSLLTGIETAINTEQYADLKQYFHENLHVTTINQNSITTPEGIDAYFKEW
                     FGEGGYLKKLDIQLNADGLTQLHGNADDPNWGLVYGTGIENYQLADGRFLPMKTRWTA
                     TVTKDANGKWRILALHIGANFYDNPIFHAVKASTKYYAAGGLVIGVLLGALGMAWFRR
                     KKQSTLV"
     gene            4212..5240
                     /gene="recA"
                     /locus_tag="PKL42_06905"
     CDS             4212..5240
                     /gene="recA"
                     /locus_tag="PKL42_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNU66844.1"
                     /translation="MDDNKAKALTAALAQIEKQFGKGSIMRMGDSDIGEDIQAVSTGS
                     LGLDIALGIGGLPRGRVIEIYGPESSGKTTLTLSVIAQMQKMGGTAAFIDAEHALDPQ
                     YAAKLGVNVPDLLISQPDTGEQALEIVDMLVRSGSVDIVVVDSVAALTPRAEIEGEMG
                     DSHMGLQARLMSQALRKLTGNIKRTNTMVIFINQIRMKIGVMFGNPETTTGGNALKFY
                     ASVRLDIRRTGAIKKGDEVTGSETRVKVIKNKVAPPFKQAEFDIMYGEGISRLGEIIE
                     LGSNLKFVEKAGAWYSYNGEKIGQGKENAKEYLREHPAIAAEIEAKIRANAKQLAEGM
                     TAARTEDD"
     gene            5259..5714
                     /gene="recX"
                     /locus_tag="PKL42_06910"
     CDS             5259..5714
                     /gene="recX"
                     /locus_tag="PKL42_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HNU66845.1"
                     /translation="MKFQPKSLRQRALDYLAKREYSYAELGQKLKAFAEETDDIPAIL
                     DDFKQRGWLSDARFTEQIVHARKAKFGSAKVANELREKGVADNLIADAVKKLKETELE
                     NALAIWRRKYKASPLNREEWAKQARFLQSRGFGFDLIKKVLNSKFENDN"
     gene            5704..>7888
                     /gene="alaS"
                     /locus_tag="PKL42_06915"
     CDS             5704..>7888
                     /gene="alaS"
                     /locus_tag="PKL42_06915"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNU66846.1"
                     /translation="MTIKLSNNPSSNEIRQAFLDYFASKGHEIVASSSLVPHGDPTLL
                     FTNAGMNQFKDVFLGFDKRPYTRATSSQKCVRAGGKHNDLENVGYTARHHTFFEMLGN
                     FSFGDYFKEDAIKYAWELLIEVFKLPKDKLTVTVYAEDDEAYDIWHKQIGVPAERIIR
                     IGDNKGARYASDNFWMMGDTGPCGPCTEIFYDHGEHHFGGPPGSPDEDGDRFIEIWNN
                     VFMQFNRDEAGVMHPLPKPSVDTGMGLERISAVLQHVHANYEIDVFTTLIAAAARETK
                     TTDLTSPSLKVLADHIRACSFLIADGVIPGNEGRGYVLRRIIRRAIRHGYKLGVRSAF
                     FHKIVPDLIAEMGSAYPELVSNQARITDILRQEEERFFETIENGMAILEAELANTKKI
                     FNGDTAFKLHDTYGFPLDLTADICRERHVSVDTVAFDAAMARQKEQARSAGKFKMATN
                     LEYTGVATTFKGYESLETMGKITALYKDGTAVNRLSEGELGVVVLDNTPFYAESGGQI
                     GDNGVLKSDNGIFSVEDTQKIQAAVFGHHGVLKTGTLNVDDSVKSNVDLSSRNATMRN
                     HSATHLMHKALREVLGEHVQQKGSLVDADKTRFDFVHNAPMSDDQIAKVEAIVNAEIL
                     TNAACQARVMDIESAQKTGAMMLFGEKYGDEVRVLDIGSSRELCGGTHVNRTGDIGLF
                     KITSESGVAAGVRRVEATTGEGALKLINAQQSLISTIAAEIKAPIN"
BASE COUNT         2313 a         1836 c         1709 g         2030 t
ORIGIN      
        1 caaaatagca tgcgcgtcat cttgtaaatt ggcttgctta cccacaaacg tcatccagcg
       61 cactgcccgt accagcacga ttggcgcagg gggtgttaaa tccatcaccg gtaaaaattt
      121 gtgatgtgca acgtttttaa aaggggttgt tgtaaactca ttggttaaat ccagcaccgc
      181 cagccacctt gtttggaaca tgtttttgtc tggaaaagca cctaaccaaa caccattttt
      241 tatttcaaca gggtgcctat tgttgcgcgt gtaaacacgg taactcagcc aactacccag
      301 caaatacggt gccagcatga gccgcgcagg ccaacgcatc acgccctgat ggcgctgaaa
      361 cacttgcttt tcaaagccaa aataggcggc tgcaacgagc gcaagggcaa tcgccaccca
      421 actaaacagc aaggtaaaaa aactagacaa ttgctttaaa tataagcctg ccatcagcgc
      481 cactacggca cccatgacat aacgccaagc catcagaatc atgcggtggt tgggcaattt
      541 tttgcgccag taaaatggtc gatcgggaat aaaatataaa caggcaacgc ctgctaacgc
      601 ccctgtccat acatcaataa aatggtgctg atacaccaac aacaccgatg caccaatggc
      661 aaaaagccaa gtgtgtaacg cgagccgcca aaagccctgc aagcgtttgg cgtacaccac
      721 ccacaacaca attaacaagc taatatgtaa cgatggcgct tggttataag gcttatcaaa
      781 gccgagtaaa attgtctgta aaaagccatt aaagccatca atcttgggga cagcaaatga
      841 aaattgcaat ggaaatagca aaaatccaat cagtgaaatg gtcgtggcaa gcaatagccg
      901 tagcgcatgc ctatctaatg cgtgacgctt gcgaaataaa aacagtgacc ctgcataaaa
      961 cgcgtcaatc gacatatacg gcaacatcat ccacggcaca aacggaatgg cagcctccca
     1021 agccgcaacc gccacaccca catcggtacg tgtgctagtg aattgattga gccagccata
     1081 actcaaaaag aaaaatggcc caaaaaacgc cagccaaagt gcgccacgtt tccaaagtat
     1141 tggatttttt tgtttttgca tcacattgtt gttttaaaca gcagaaattt tatacatcac
     1201 tcaatcatca ttacgcacta caacctgcta ctttattttt aaaaaataac cattaaattt
     1261 tgcttaaatt ttaagcattt taatacaaaa attcgcccca acctttttcg atgctctgta
     1321 agccttgtaa ataaaggctt acagaggaga aattttttct cgatgatttt aaatcaattt
     1381 taaataaaat tattagtcta gtttttttgc caatgaaaca ctaaaaattc cccacgtatc
     1441 tgaccattgg tcaatttttt caaaaccggc attcgccacg agttgatcca tttcgcgttg
     1501 cgtgcggcgg cgcataatcc acgctttatt ttgacgatgg ctggtgagcg tacgcgcaat
     1561 tagctctaac tgtgggtgcc acggttgatt ggtgtaaatc aaatatccac ctgcttctaa
     1621 ggcttgatgc acgccagcca gtgaggcacc tatcggcgca ttttcaggaa aaagctcata
     1681 taaccctgaa acaatggcga tggttggctt gggggtaacg gttgcgatgg atttttcatc
     1741 aaaagcatcg gctttttcaa actgtgcttt acttgccaat cctaattttt gtatgagttc
     1801 gcgcccgccc gcgacattca agtcgctata atcacgtagc aatacatggt ctgctttcac
     1861 ggccactgct gcctctagcg cgtagcggcc atgccctgcg gcaatgtcca tgatgcgcac
     1921 ttctttggca ttttcacgta acaaatgtgc cgccttggcg agcaaggctt ctatatgttg
     1981 cttacgcacg cgtatgccac gccagccgat ggcatttaag taagtgcggt caatgatttc
     2041 acctaaagca ccgccaataa acgttgtact acgcgcttga ttttcataca cataatctaa
     2101 ggtgctgcct gaatcaaacc ctgtatccac ccctaagcgc aggccacgcg ataactgact
     2161 gccaaatcga atgttggcgc gcgtagcggc aaaatagaga ttttttaaac taaaggttgg
     2221 taatggtttt ttaagttgct ctgcttcatc aaaggtgtag ccagcctggt cagcgttgat
     2281 taaatcttgt gatgcaaagg gtgttgcaaa ttgcgcggct ataaattcac gcgcttttgc
     2341 taatggcaaa tggcggtctt tttcgccaag tgtgtcgtga aaaaagcctt caaatacatg
     2401 atactctttg gtttttgccc ccaaattttg gtacaaatca cgcaccggtg tttgatgcac
     2461 cacccaatcg ctgcccgaaa caagaaactg ggttggtgtg gttatggctg cggcatcttt
     2521 taccaagcgc tctgcggttt catataactc cagcaatata ttcaccgcaa tcgggcgagt
     2581 gatgaggtga tcgcgctcat acgatgcaat gcgcgcttca tcatgcgtca gccattttgc
     2641 tttaacatat gaattaacaa aagcgagtgc tggtaccaat tttgttagcc ctaatttttg
     2701 caacagacca agcgccgtac gtgcaaacgg cacatacaat ttcactttta acgcaggcga
     2761 agccaacacc aaacaacgaa tattaggtgc ataatcatgt acccaagcgg cagcaatcac
     2821 accgccaaca ctttgcgcaa tgacggcaat attttccaac ttaaagccat actgacggca
     2881 aatatggtgc gcaaagtcat cagcgtcttt cacaaatacg ccaaaatttt ctgcatagcc
     2941 gcgtacgccg ggtgacctac ctagcccgcg cgcatcccaa gcaaaacaat cgtaatctgg
     3001 catattgagg ctatccacca cctcttgttg gcggcctgag tgttcatgcc cacggtgaaa
     3061 taaaatgacg gcacgttttg tatcatttac ttttgaagct tggccctcac ttggccaatg
     3121 acgataaaaa atttcggcgt tatccaccgc cgtaaacgtg cgctcaagtg caactctacc
     3181 cattcttact cccatttttg gtgatttata ttgaacagcc atctttgaca taactatata
     3241 attagttacg tagtgatata tgtcaagtgt tttttatttt tttaaataat tttagtgtga
     3301 gatttttaaa cgagggttga ttgcttttta cgacgaaacc aagccattcc tagcgcgcca
     3361 agcaatacgc ctattactaa accgccggca gcataatatt tggtagacgc ctttaccgca
     3421 tgaaaaatcg ggttgtcgta aaagttggcc cctatgtgca aagccaaaat acgccacttg
     3481 ccgttggcat ctttcgtcac agtcgcagtc cagcgtgttt tcattggtag aaagcggccg
     3541 tccgctaact ggtaattttc aatgccggtg ccgtacacta aaccccaatt ggggtcatca
     3601 gcattgccat gtaactgggt aagtccatcg gcattcaact gaatatccaa ctttttcaaa
     3661 taaccgcctt cgccaaacca ttctttaaaa taggcatcaa tgccttctgg tgtggtgata
     3721 ctgttttgat tgatggtggt gacgtgtaaa ttttcatgaa aatattgctt taagtctgca
     3781 tattgctcag tgttaatcgc cgtttcaatc cccgttaaca gactacgcaa ctcctgatgt
     3841 atcgcttggt tagccgcgtc ttgaactgcc ccatcttggc tagaaatttg ctgaatattt
     3901 gcttctgacg gattctcacc ctcgtcagcc agcaagttaa ctgtaaacag cattaaaaaa
     3961 atggcgagtg ctcgtatcaa catgttgaat ctccaatggg ttacacaaag tgcgcagcat
     4021 gcaaaattaa attgcaagcg acaatatcta cgttctaata cctgacagtt tacagaaatt
     4081 ctaagctaaa aatgaatgcc gtatgcatta ggcacaaatt tagccctaac tactgatggc
     4141 gcttacttaa ttgcacattt tgtgaaataa ttagcagtta attcagcacc attatataaa
     4201 ggcaaaaacc catggatgac aacaaagcta aagccctcac cgcagcactt gcacaaatcg
     4261 aaaaacagtt tggcaaaggc tccattatgc gcatgggaga tagcgatatt ggcgaagata
     4321 ttcaagcagt ttccacaggt tcactcggct tagatatcgc cctcggcatt ggcggcttac
     4381 ctcgcggacg cgtcattgaa atttacggcc ctgaatcctc aggtaaaacc acgcttacat
     4441 tatccgtcat cgcccaaatg caaaaaatgg gcggtacggc agcgtttatt gatgctgaac
     4501 acgcactaga cccacaatat gcggcaaaat tgggtgtcaa cgtgcctgac ctcttaatta
     4561 gccaaccgga taccggcgag caagcgcttg aaatcgttga tatgctcgtg cgttcaggct
     4621 cggttgatat tgtcgtggtt gactcagtag ccgccctcac cccgcgcgct gaaattgaag
     4681 gtgaaatggg tgatagtcac atgggcttac aagcgcgttt gatgtctcaa gccctgcgta
     4741 aactcaccgg caacattaag cgcaccaata ccatggtgat tttcatcaac caaattcgta
     4801 tgaaaattgg cgtgatgttt ggcaacccag aaactaccac cggcggcaac gcactcaaat
     4861 tttacgcttc ggtacgttta gatattcgcc gtaccggtgc aataaaaaaa ggtgacgaag
     4921 tgacaggttc tgaaactcgc gtaaaagtga ttaaaaacaa agtcgcaccg ccatttaaac
     4981 aagctgaatt tgacattatg tacggtgaag gtatttcacg tttaggcgaa atcattgaac
     5041 ttggttcaaa cctgaaattt gttgaaaaag cgggtgcttg gtatagctac aatggcgaaa
     5101 aaattggcca aggtaaagaa aatgccaaag aatatttacg tgaacatcca gccattgcag
     5161 cagaaattga agctaaaatc cgcgctaatg ccaaacagct cgctgaaggc atgacggcag
     5221 cgcgcaccga agatgactaa aatcagggac gattcggtat aaagtttcaa cctaaaagtt
     5281 tacgacagcg tgcgttggat tatctagcaa aacgcgagta ttcatatgct gagctagggc
     5341 aaaaattaaa agcctttgct gaagaaactg acgatattcc tgccatttta gatgacttta
     5401 aacagcgtgg ctggctttca gatgcgcgtt ttaccgagca aattgtccat gctcgcaagg
     5461 ctaaatttgg cagtgcaaaa gtagccaatg agttacgtga aaaaggtgtc gcagataatt
     5521 tgattgcaga cgcggttaaa aagttaaaag aaacagaact ggaaaatgcg ctagccattt
     5581 ggcgcagaaa atataaagcc agcccgctaa accgtgaaga atgggcaaaa caagcaagat
     5641 ttttacaaag tcgcggtttc ggctttgatc ttattaaaaa agtcttaaat agtaagtttg
     5701 aaaatgacaa ttaaattaag taacaaccca agtagcaacg aaattcgcca agcctttctt
     5761 gactacttcg cctcaaaagg ccatgaaatc gtagcatcca gctcacttgt accacatggt
     5821 gacccaacct tgctatttac caacgcgggt atgaaccaat ttaaagacgt gtttttgggt
     5881 tttgataaac ggccttacac gcgcgcaact tccagccaaa aatgcgtacg cgcaggcggc
     5941 aagcataacg acctcgaaaa cgtgggctac actgcgcgcc accacacatt ctttgaaatg
     6001 ttgggcaact tcagctttgg cgactatttt aaagaagatg ccattaaata tgcttgggaa
     6061 ttgctcatcg aagtattcaa actgcccaaa gacaaactca ccgtcacggt atatgcagaa
     6121 gatgatgagg cttatgatat ttggcataag caaatcggcg tgccagccga gcgcattatt
     6181 cgcattggcg acaacaaagg cgcgcgttac gcttctgaca acttctggat gatgggtgac
     6241 actggcccat gcggcccatg taccgaaatt ttctacgacc acggtgaaca ccattttggt
     6301 ggccctcctg gcagtccaga tgaagatggc gatcgcttta ttgaaatttg gaacaacgtg
     6361 ttcatgcagt ttaaccgcga tgaagcgggc gtgatgcatc cgctccccaa gccatctgtg
     6421 gacactggca tgggtttaga acgaatttcg gccgtattgc agcacgtaca tgccaattac
     6481 gaaattgatg tttttacgac cttaatcgcc gccgccgcgc gtgaaactaa aaccaccgat
     6541 ttaaccagcc caagcctaaa agtgctggct gaccatattc gtgcttgttc tttcttaatt
     6601 gctgatggtg tgattcctgg caacgaaggc cgtggctatg tactgcgccg tattattcgc
     6661 cgcgccattc gtcatggcta caaactcggt gtacgcagtg cattcttcca taaaatcgtg
     6721 cctgatctaa tcgcagaaat ggggagtgct tacccagagc tggttagtaa ccaagcaaga
     6781 ataactgata tcctcagaca agaagaagaa cgtttctttg aaaccattga aaacggcatg
     6841 gcgattttgg aagctgagct tgccaatact aaaaaaatat ttaatggcga tactgcattt
     6901 aaattgcatg acacctatgg atttccgctt gatttaacgg ctgacatttg tcgtgaacgt
     6961 catgtgagcg tagacacagt cgcttttgat gccgccatgg cacgccaaaa agaacaagcc
     7021 cgctccgctg gcaagttcaa aatggcaact aaccttgaat atactggcgt agcgacaact
     7081 tttaaaggct acgaatcatt agagacaatg ggcaaaataa ctgcgctcta caaagatggt
     7141 acagcagtca atcgactctc tgaaggtgag ctaggagttg ttgttctcga taatacgcca
     7201 ttctatgcag aatctggcgg tcaaattggt gataacggcg tattaaaatc tgacaatggt
     7261 attttttccg ttgaagacac ccaaaaaatt caagccgcag tttttgggca tcacggcgtg
     7321 cttaaaacag gcacattgaa tgtagatgat tccgtcaaat cgaatgtgga tttatcaagc
     7381 cgtaatgcca ccatgcgcaa ccattcagca acccatttga tgcataaagc cttacgtgaa
     7441 gtattaggtg agcatgtaca acaaaaaggc tcgctggtag acgctgataa aacgcgtttt
     7501 gactttgtcc acaatgcgcc catgagcgat gatcaaattg caaaagtaga agccatcgtc
     7561 aacgctgaga ttttaacgaa tgccgcttgc caagcacgtg tgatggatat tgaatctgct
     7621 caaaaaactg gcgccatgat gttgtttggt gaaaaatatg gtgatgaagt tcgcgtgcta
     7681 gatatcggca gttcgcgcga gctttgcggc ggtacacatg tgaaccgcac cggcgatata
     7741 ggcttattca aaatcacctc tgaaagtggc gttgctgcag gcgtgcgtcg tgttgaagcc
     7801 accactggag aaggtgcatt aaaactgatt aacgcgcagc agagtttgat ttcaaccata
     7861 gcggctgaaa tcaaagcacc catcaatg
//
LOCUS       DAOQSI010000094         7818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_8922_length_7818_cov_6.621409, whole genome shotgun sequence.
ACCESSION   DAOQSI010000094 DAOQSI010000000
VERSION     DAOQSI010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 7818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7818
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8922_length_7818_cov_6.621409"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..1093)
                     /gene="waaC"
                     /locus_tag="PKL42_06920"
     CDS             complement(134..1093)
                     /gene="waaC"
                     /locus_tag="PKL42_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HNU66847.1"
                     /translation="MLKLLIVKTSSLGDVIHNLPIIHDICHHIADIEIDWVVEESFAD
                     IPKLHADVNRVIPVAMRRWRKSIFSKKTWQEIKLAKQQIQQKQYDIVLDTQGLLKSAF
                     ICKLAHGIKHGYDKHSIREPIASRFYDVTHACTYQQHAVIRNRTLAALSLGYPVPTGA
                     PNYGLGEALFTVNLPKPYVTGLHGTSRDSKLWPLQHWIQLGKALADQGLHLVLPWASD
                     EEESRANVIAISLNNATVLPKCTIEELAVIICQAQAAVGVDTGLSHLAAALNVPTVAI
                     YTDTDPALTGVMAGAFQPAVNLGGKAQLPSVDEVMRTLLTFIK"
     gene            1155..2339
                     /locus_tag="PKL42_06925"
     CDS             1155..2339
                     /locus_tag="PKL42_06925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNU66848.1"
                     /translation="MKFAFLIFKVFPYGGVQRDMLRIAHVCAAAGHEVTIFTGEWRGE
                     MPEARIKVQLLPSSGWMNHQKHQSLIHAMQQVLVKNPVDLVVGFNRMAGLDAYYAADP
                     CYVERTHKKGFFYKLTGRYRFFAEAEKAVFGKESQTQILLLTAREKQSFQQFYQTPDE
                     RFYLLPPSIPLEKFAGKNRKQCRQYVRAQFNLPENANIILTVGSAFVRKGVDRTIEAL
                     AALPKALQDNTWLIAIGEYESKSDMQTYAKDRHIAHRCIQAGGRDDVADLMVGADVLA
                     HPARSELAGIVLIEAMTAGLPVLVTDVCGYAPHIALANAGVVLTAPYQQESMNAALAD
                     MLQSEQKTAWQINGEQYTRHIKNTTSATAEADYLITLANKKLPRPQAGEGWGEGVLNE
                     ST"
     gene            2326..3135
                     /gene="rfaP"
                     /locus_tag="PKL42_06930"
     CDS             2326..3135
                     /gene="rfaP"
                     /locus_tag="PKL42_06930"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HNU66849.1"
                     /translation="MNQLNLPESLRPIFGADPFAEIMQMQGKVFRDVRGRKTIQVTLG
                     DKSYFIKQHFGVGWGEIFKSYLSFKEPVLGAMTEVRAIQKLDEIGIPTTPLVGYGVQG
                     NNPAKQQSFIITQDLGDITSLEDVCADWKTNPPDAQFKQQVMIKMAQLATKMHGAGLC
                     HRDFYLCHLALKKSEIAQGEVNLILIDLHRMMLSQPSGGGAVIKDIAGLIFSTMDCGF
                     TDHDWALFKQHYLPQTAHFWAKAEARANQLYAKYHSKKFQQRLSTEKSKMH"
     gene            complement(3284..4126)
                     /locus_tag="PKL42_06935"
     CDS             complement(3284..4126)
                     /locus_tag="PKL42_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNU66850.1"
                     /translation="MLTLLSKTLACLSLPMIHRLGAALGLLLHWCTPASAQLQHKNLQ
                     QSHICANETEISHVIHANIAETGKAILETLAIWQWPQEKALSLVKACHNWHLVEEALA
                     RGKGIIFLTPHLGCFEITSIYYAAKNPITVLFRPPKKSWLAPLIDSGRSKGHVTLAPA
                     NAQGVRAILQALKRGEAVGILPDQIPNEGEGEWADFFGKPAYTMTLASKLAEKTGAAV
                     IMAFGERLANGEGYEVHLSAVESIATPALLNQAIETQIRQKPAQYLWRYHRYKVRRGS
                     LPKA"
     gene            4228..5007
                     /locus_tag="PKL42_06940"
     CDS             4228..5007
                     /locus_tag="PKL42_06940"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HNU66851.1"
                     /translation="MLNFISAKHAQLLQKNQLDTFEKVWAYKVDWFEEPNERRGGWSG
                     VGRISIAQEDGNAIGAFLKKQENHCRTSFLHPIRGVPTFQREFEMMQYLASKNIRAPE
                     VLLFGRNPQGDLKTTLMTRELLGFQSLEALTETLFANESPGKTTQRALAKAVAKFARQ
                     LHLAKVQHRSFYPKHLFVNFSNQAAPEVAVIDLEKSRINWLPVLRTLIDLSVLNRHAK
                     YWSKTTRMYFYLQYFGIKRLTPYSKWLCRLIIKRSNRVKHK"
     gene            5065..5745
                     /locus_tag="PKL42_06945"
     CDS             5065..5745
                     /locus_tag="PKL42_06945"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNU66852.1"
                     /translation="MSKPKEAPIKLEFSNKYTREHSQYYYEKHQRGLEPRLSHWRDVQ
                     IARKALKIAGEPKEVLDLPCGAGRFWPTLAENPNRKIIGADNSADMVAVALEHCPKEI
                     ASRVTAFQTSAFAIDMQDKAVDSIFSMRLMHHIGQSEHRIAMLKEFHRVTKDTVILSL
                     WVDGNLKAYQRRKSEERHPRGPNQNRFVIPAKQIEQEFGQAGFNIVDYIDFLPYYAMW
                     RVYILRKA"
     gene            5755..7179
                     /locus_tag="PKL42_06950"
     CDS             5755..7179
                     /locus_tag="PKL42_06950"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HNU66853.1"
                     /translation="MQILLKNAPPIEVEAVVRTVPHQREVVRGFWNHQAVYAKKFTGP
                     RAKIHFERDIAGVQLLVKADIATPARLFEGETESAGGFVAIFAAIDKSQNAEVVYALL
                     DKNARLSLMQQLVKVVAQHHQAGLIQTDLYFKNFLVETKTQAIYTLDGDGIRPLNTLF
                     KTKQKLSNLATLFSKMDVQDDVWIADLTQRYCTQTNMQFSAELAAAVWAQTQKIRAQV
                     SRNYADKKVFRNCTDVKVIQNFSRFAATARDFVVEEKTLTELDAYLENPTQNIKNGNT
                     CTIAKAEIAHRAVVIKRYNIKGLSHKVSRAFRKSRAATSWANAFRLQMANIATPKPLA
                     LFEERFGVLRARAYFVSEFVDAPDIAQFFAQTTDIETKQQVALEVAKLFYQLYLLKIS
                     HGDCKATNIKIKDGKPLLLDLDAMQANAWNFESKHVKDLQRFMRNWQHAAALTTLFKD
                     AFVQVYDEADDVFAPSILSRAGVI"
     gene            7382..>7818
                     /locus_tag="PKL42_06955"
     CDS             7382..>7818
                     /locus_tag="PKL42_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HNU66854.1"
                     /translation="MIVLGLSGALGHDASAAILVDGKIIAAAEEERFIRDKHAKNKFP
                     YEAAKFCLKQAGIQPEEVDIVAFPYAEIPLSSNARWHYAKRHWYAPDRALDALFNGNR
                     RFRRNRDKSLKLMADLGLINAKFVPVEHHLAHASSAYHLSGFK"
BASE COUNT         2175 a         1647 c         1849 g         2147 t
ORIGIN      
        1 gattttttgt gcctctgtga gccttataaa taaaggcttg cagagcaggt caaaagggtt
       61 gtgctaaaaa acttgttttg aattaacttg tttttagttc aatagcgtac gcttaatttt
      121 tttgcagcgc taattatttt ataaacgtta aaagtgtgcg catgacttca tccacacttg
      181 gtagttgtgc ttttccgcct aaattcactg cgggctggaa cgcgccagcc atcacgccgg
      241 tgagcgctgg gtcggtgtcg gtgtaaatag caacggttgg cacattaagg gctgcggcaa
      301 ggtgcgataa acctgtgtct acgcctacgg cggcctgagc ttggcagata atgacggcaa
      361 gctcttcaat ggtacatttg ggcaatacgg tggcattgtt gagtgaaatg gcaatcacat
      421 tggcgcggct ttcttcttca tcactcgccc aaggcagcac taaatgcagg ccttgatcag
      481 cgagcgcttt gcctagctga atccaatgct gtaacggcca caatttgctg tcgcggcttg
      541 tgccgtgcag gccagtcaca taaggttttg gtaagttcac ggtgaataac gcttcaccta
      601 aaccgtaatt aggtgcgcca gtcggcactg ggtaacccaa gctcaacgcc gccagcgtgc
      661 gattgcgtat tacagcgtgc tgctggtacg tgcacgcatg ggtgacgtca taaaaacggc
      721 tggcaattgg ctcgcggatg gagtgtttgt cgtaaccatg tttaatgccg tgcgctaatt
      781 tgcaaataaa ggcgcttttg agcaggcctt gcgtgtcgag gacgatgtcg tattgttttt
      841 gctgaatttg ctgtttagca agcttgattt cttgccaagt ttttttgcta aaaattgact
      901 tgcgccagcg acgcattgcc acaggaataa cacggtttac gtctgcgtgc agcttaggaa
      961 tgtccgcaaa actttcttct accacccaat ctatttcaat atcagcaata tgatgacaaa
     1021 tgtcatgaat aatcggtaag ttgtgaatga catcgcctaa tgacgaggtt ttaacgataa
     1081 gcagtttcag cataaacgtc attttcgcat acaattaggt tttttcatca cctgcaaaaa
     1141 agtcgtctat tccattgaaa tttgcgtttc ttatttttaa agtgtttccg tacggcggcg
     1201 tgcagcgcga tatgttgcgg atcgcgcatg tctgcgcagc tgcagggcat gaagtcacca
     1261 tttttacagg ggaatggcgc ggtgaaatgc ctgaagcgcg gattaaagtg caactattgc
     1321 ccagcagcgg ttggatgaac caccaaaagc atcaatcact cattcatgcg atgcagcagg
     1381 tactagtaaa aaatcctgtg gatttagtcg tggggtttaa ccgcatggct gggctagatg
     1441 cttactatgc ggctgacccg tgctatgtgg agcgtacgca caaaaaaggt tttttctaca
     1501 aactcactgg gcgctatcgc ttttttgcag aggctgaaaa agcggtattt ggaaaagaaa
     1561 gccaaactca aattttattg ctcactgcgc gtgaaaaaca aagttttcag cagttttatc
     1621 aaactcctga cgaacgcttt tatttattgc cgcccagtat tccacttgaa aaatttgcag
     1681 gcaaaaaccg caagcaatgt cgccaatacg tacgggcgca gtttaacttg cctgaaaacg
     1741 ccaacatcat tttaacagta ggctcggctt ttgtgcgtaa aggtgtggat agaaccatag
     1801 aggcgctcgc ggcactgcct aaggctttgc aagacaacac ttggctaatt gcaattggtg
     1861 aatacgaatc aaaaagtgat atgcaaacct atgcaaaaga ccgccatatt gctcaccgct
     1921 gcatacaagc aggcgggcgc gatgatgtgg ctgatttgat ggtaggtgct gatgtgctag
     1981 cgcatcctgc gcgctctgag ttggcaggca ttgtgctgat tgaagccatg actgctggct
     2041 tgcccgtgct ggtgaccgat gtgtgtggtt atgcgccgca tattgcactg gccaacgctg
     2101 gcgtggtttt aacggcgccc tatcagcaag aatcgatgaa tgcggcttta gctgatatgc
     2161 tgcaatctga gcaaaaaacg gcgtggcaaa tcaatggcga gcaatataca cgacatatca
     2221 aaaacaccac ttcggcaact gcagaggcgg attatttaat tacattagca aacaaaaaac
     2281 tccctcgccc gcaggcggga gaaggttggg gtgaaggtgt attgaatgaa tcaacttaat
     2341 ttacctgaat cccttcgccc aatttttggt gcagacccgt ttgcagaaat catgcaaatg
     2401 cagggtaaag tttttcgcga cgtgcgtgga cgcaaaacca tacaagtcac gctaggggat
     2461 aaaagctact ttattaaaca gcatttcggt gtgggctggg gcgagatatt taaaagttac
     2521 ttgagtttta aagagcctgt gcttggcgcg atgactgaag tgcgggcgat tcaaaaattg
     2581 gatgaaattg gcatccccac tacaccgtta gtgggttatg gcgtgcaggg caacaaccca
     2641 gctaagcaac agtcatttat tattacgcaa gatttaggcg acattacctc gcttgaagat
     2701 gtgtgcgccg attggaaaac taatccccct gatgcgcagt ttaaacaaca agtgatgatt
     2761 aaaatggcac aactagctac aaaaatgcac ggtgcaggac tatgccatcg tgatttttat
     2821 ttgtgccatt tggcgctcaa aaagtcagaa atagcacaag gcgaggtgaa cctgatttta
     2881 attgatttgc atcgtatgat gctaagtcag ccaagtggtg gaggtgcggt aataaaggat
     2941 attgcaggct tgattttttc taccatggat tgcggtttta ccgaccatga ttgggcgtta
     3001 tttaagcaac attatttgcc acaaactgcg catttttggg caaaagccga agcacgtgcc
     3061 aaccaacttt atgctaaata tcacagcaaa aaatttcagc aacgcttatc tacagaaaag
     3121 tcaaaaatgc actaaaaaaa cagttaaatc cacctgtttt tttgcgcaac ctttttcgat
     3181 gctctgcgag ccttataaat aaaggctcgc agagcattaa atttttctcg agggaaatat
     3241 taagcgaaat atagttttta tagattggca atcaatgcca gttttacgct tttggtaagc
     3301 taccgcgccg cactttataa cggtgataac gccacaaata ttgcgctggt ttttgcctga
     3361 tttgggtttc gatggcttgg ttgagtaaag cgggtgttgc gatgctttct acagccgaaa
     3421 gatgaacttc ataaccttcg ccattggcca atcgttcacc aaatgccatg atgactgccg
     3481 cacccgtttt ttcagccaat ttgctggcaa gcgtcatggt gtaggcgggt ttgccaaaaa
     3541 aatccgccca ttcgccttcg ccttcattcg ggatttggtc tggcaaaatg ccgaccgctt
     3601 cgccgcgttt taaagcctgt aaaatcgcac gcacaccttg cgcattggcg ggcgcgagcg
     3661 tcacatggcc tttgctgcgc cctgaatcaa tcaatggggc aagccaagat ttttttggcg
     3721 gacgaaacag tacggtaatc ggattttttg cagcgtaata aatagaggtg atttcaaaac
     3781 agcctaaatg cggagtcagg aaaataatgc ctttgccacg cgctaaagct tcttctacca
     3841 aatgccagtt gtggcaggct tttaccagcg acagggcttt ttcttgcggc cattgccaaa
     3901 tcgccaatgt ttctaaaatc gctttgcccg tttcggcaat gttggcgtga atcacgtgac
     3961 taatctcggt ttcattcgcg caaatatgac tttgttgtag atttttatgc tgcaattgcg
     4021 ccgatgcagg ggtacaccaa tgcaatagca agcccaatgc tgcgcctaag cgatgaatca
     4081 tcggcaagga taagcaagca agtgttttgg agagtaaggt cagcataagg tttgcgctat
     4141 tttaatggat aacggaatgg ggtgcgtaaa tttatggcgt aacctgataa aatgctcacc
     4201 aattaaaatg attgaatgga gtgggttttg ctaaacttta tttctgcaaa acatgcgcaa
     4261 ttactgcaaa aaaatcaatt agatactttt gaaaaagtct gggcatacaa agttgattgg
     4321 tttgaagagc ctaatgaaag gcgtggcggc tggagcggtg tggggcgcat tagcattgcg
     4381 caagaagacg gcaacgctat tggcgctttt ttaaagaagc aagagaatca ttgtcgcacc
     4441 tcttttttgc accctattcg tggtgtgcct acatttcagc gcgagttcga aatgatgcaa
     4501 tatttggcat caaaaaatat tagagcgcca gaggtgctgt tgtttggccg aaatccgcag
     4561 ggagacttaa aaaccacgtt gatgacccga gagttgcttg ggtttcaatc gttagaggct
     4621 ttaactgaaa cgttatttgc aaatgaatct ccaggcaaaa caacgcaacg tgcgctggca
     4681 aaagcggtgg caaaattcgc ccgtcagctc catttggcca aggtccagca tcgctcgttt
     4741 tatcctaaac atctgttcgt gaattttagc aaccaggccg cacccgaggt ggctgtcatc
     4801 gatttagaaa aatccagaat taactggctg ccggttttgc gtacgctcat tgatttatct
     4861 gtattgaatc ggcacgccaa atattggagt aaaacgacaa gaatgtattt ttacctgcaa
     4921 tattttggta ttaaacgttt aacaccttat tcaaaatggc tttgtcgatt gattatcaaa
     4981 cgttctaatc gagtaaagca caagtaattg gtataattca actattattc agcctaacta
     5041 aaattttcaa ggtaccttta cacaatgtca aaacctaaag aagcgcccat caagctcgag
     5101 ttttcaaata aatatacacg tgagcattca caatattatt acgaaaaaca tcagcgtggc
     5161 ttagagccga gactttccca ctggcgcgat gtgcaaatag cgcgcaaagc cctgaagatt
     5221 gcgggcgagc ctaaagaggt gcttgattta ccctgtggtg cagggcgttt ttggcctacg
     5281 ttggctgaaa atccaaaccg caaaatcatc ggcgcggaca actcagctga tatggtggcg
     5341 gttgcactgg agcattgccc gaaagagatt gcctcacgcg tgactgcatt ccagacatca
     5401 gcgtttgcaa ttgacatgca agataaagcc gttgacagca ttttcagcat gcgattgatg
     5461 catcatattg gccagtcaga acaccgcatt gccatgctaa aagagtttca tcgcgtgact
     5521 aaagatacgg ttattttatc tttatgggtg gatggcaatt taaaagctta tcaacgcaga
     5581 aagtctgaag aaagacatcc acgtgggccc aatcaaaacc gcttcgttat tccagccaag
     5641 caaattgagc aagagtttgg tcaagcgggg tttaacattg tcgattatat cgattttctg
     5701 ccttattacg ccatgtggcg tgtctatatt ttgcgtaaag cctaattcac gcagttgcag
     5761 atacttctaa aaaacgcgcc gccgattgaa gttgaggctg tggtgagaac cgtgccgcac
     5821 cagcgcgagg tggtgcgtgg cttttggaat catcaagcgg tatatgccaa aaagtttact
     5881 ggtccacgtg caaaaataca ctttgaacgc gatattgcag gtgttcagtt actcgtcaaa
     5941 gccgatattg cgaccccagc acgtttgttt gaaggtgaaa cagaatccgc tggcgggttt
     6001 gtggctatct ttgcggccat agacaaatca caaaatgctg aagtggttta tgcgctgcta
     6061 gataaaaatg cacgtttatc gttgatgcaa caactagtaa aagtagtcgc gcagcatcat
     6121 caagcgggtt taattcaaac cgatttatat tttaaaaatt tcttggtaga aactaaaacc
     6181 caagccattt atacactgga tggcgatggc attcgtccgc tcaatacatt atttaaaact
     6241 aagcagaagt taagtaattt ggccacgcta ttttcaaaaa tggatgtgca agatgatgtg
     6301 tggattgccg atttaaccca gcgatattgt acgcaaacaa atatgcagtt ttctgctgaa
     6361 cttgcggcag cagtttgggc gcagacacaa aaaattcgcg cgcaggtgag ccgaaattat
     6421 gcggataaaa aggtttttcg caattgcacc gatgtgaaag tgatacaaaa ttttagccgc
     6481 tttgctgcaa ccgcgcgtga ttttgtagtt gaagaaaaaa cattgactga gttagatgcc
     6541 tacttagaaa atcctacgca aaacatcaaa aacggcaata cttgtactat tgctaaagct
     6601 gaaatcgctc atcgtgcagt agtgataaag cgttacaata tcaaaggatt atcgcataaa
     6661 gtcagccggg cttttcgcaa aagtcgggcg gctacctcat gggcaaatgc gtttcgtttg
     6721 cagatggcga acatcgccac tcccaagccg ctggcgttgt ttgaagagcg cttcggagtt
     6781 ttgcgcgcgc gcgcctattt tgtaagcgaa tttgtggatg cgcccgatat tgcacaattt
     6841 tttgcacaaa cgacagatat agaaaccaag cagcaagtag cgcttgaagt ggctaaactg
     6901 ttttaccaat tatatttgct taaaatctca catggcgatt gcaaagcaac gaatatcaaa
     6961 attaaagatg gcaagccgct gttgcttgat ttagatgcga tgcaggccaa tgcttggaat
     7021 tttgagtcaa aacatgtgaa agatttacaa cgttttatgc gcaattggca acatgctgct
     7081 gcgcttacaa cgcttttcaa agatgcgttt gtacaggttt atgatgaagc tgatgatgta
     7141 tttgcaccat caattttatc gcgtgcaggg gttatttaaa cgcataacct tttgacctgc
     7201 tcgctaagcc tttattttca aggcttccag aggcataaaa ttctctcgga gcaataatca
     7261 gcttttttaa caatgcgttg ctgtacaaaa accatagtta ccattgcatt atctttaacg
     7321 attttgccta aacacagtaa aatagcacca ttcttatcaa aatcactttg aaagtttcac
     7381 catgattgtt ttaggtttat caggcgcatt ggggcatgat gcttcggccg ctattttagt
     7441 agatggcaaa attattgccg cagctgaaga agagcgcttt attcgcgaca agcatgcaaa
     7501 aaataaattt ccttatgaag ccgcaaagtt ttgcttgaag caagcaggca ttcagcctga
     7561 agaagtcgat attgtcgcgt ttccgtatgc ggaaattcca ttgagtagca atgcgcgttg
     7621 gcattacgcc aagcgtcact ggtatgcgcc agaccgtgcg ctagatgcat tatttaacgg
     7681 taatcgccgc tttagacgta accgcgataa atcgctcaaa ttgatggcgg acttgggctt
     7741 aattaacgct aagtttgtgc cggttgaaca ccatttggcg cacgcttcga gtgcttatca
     7801 tttaagcggg tttaaaga
//
LOCUS       DAOQSI010000095         7812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_8931_length_7812_cov_5.952301, whole genome shotgun sequence.
ACCESSION   DAOQSI010000095 DAOQSI010000000
VERSION     DAOQSI010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 7812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7812
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8931_length_7812_cov_5.952301"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PKL42_06960"
     CDS             <1..424
                     /locus_tag="PKL42_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HNU66855.1"
                     /translation="PEHELEELTGIYVKRGLTSDLAKQVALQLTAHDALGAHARDELG
                     IIEATNAKPVQAALASAGTFAVGALLPLLVAWFASETKLVLSVAVMSLVFLALLGGLA
                     AKAGGASIWQGTARVTFWGALAMLATAGVGSLFGVAIA"
     gene            complement(547..1095)
                     /gene="ppa"
                     /locus_tag="PKL42_06965"
     CDS             complement(547..1095)
                     /gene="ppa"
                     /locus_tag="PKL42_06965"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HNU66856.1"
                     /translation="MSLNQVPSGKNLPHDFNVIIEIPMQGDPVKYEVDKESGAIFVDR
                     FMGTSMQYPCNYGYIPHTLADDGDPVDVLVITPVPLLPGVVVRCRALGMLQMTDEAGG
                     DAKLLAVPVEKVCGLYGYQKTYKDVSPWRLEMISHFFEHYKDLDKGKWVKIEGWVGID
                     DAHKEIMDGVARYNNAEVKPAF"
     gene            complement(1197..1469)
                     /locus_tag="PKL42_06970"
     CDS             complement(1197..1469)
                     /locus_tag="PKL42_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HNU66857.1"
                     /translation="MFDKFKTLYRHLKKELEVYRLVLKHPETPWMAKLLLGLAVGYLL
                     LPFDLIPDFIPIIGQLDDVVIIPALFYLALKLTPQEIIQQCRAQVS"
     gene            complement(1462..2358)
                     /locus_tag="PKL42_06975"
     CDS             complement(1462..2358)
                     /locus_tag="PKL42_06975"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNU66858.1"
                     /translation="MSSPLLYSNITSLHKLNQGKVRDIYAVDANQLLLITTDRLSAFD
                     VILPTPIASKGAVLTQIANFWFDKLKHIVPNHLTGIDPATVVSADEKSQVHGRAIVTK
                     KLKPLPIEAIVRGYLVGSGWKEYQAKGTVCGIALPANLQEASQLPQPLFTPSSKAEIG
                     DHDENISLEKCATLLGKELAEKVAKVSVQLYTEAAQYALTRGIIIADTKFEFGVDDAG
                     TLYLIDEILTPDSSRFWPADGYVVGKNPPSFDKQYVRDWLESTGWNKTPPAPALPADV
                     AQRTSEKYLEAFERLTGKKLDV"
     gene            complement(2377..2799)
                     /locus_tag="PKL42_06980"
     CDS             complement(2377..2799)
                     /locus_tag="PKL42_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HNU66859.1"
                     /translation="MKTSSQIGKAGQEAIKYVEQLVLVVIGMATVYATFDEIYKMWVT
                     SKVGVGDLLLLFLYLEVLSMVKQYLGSGKLPVRYPLYIGIVALARYLVLEVKTLTPEH
                     ILAVATAVLIIALAVLAVRFGHVRFPYTEDGDNPNVPK"
     gene            complement(2856..4289)
                     /gene="pyk"
                     /locus_tag="PKL42_06985"
     CDS             complement(2856..4289)
                     /gene="pyk"
                     /locus_tag="PKL42_06985"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNU66860.1"
                     /translation="MTLRKTKIVATLGPSSSSEEMLARMITAGVNVVRLNFSHGTAEE
                     HIKRAEIVRSIAAKLGRPVGVLCDLQGPKIRIGKFELGKVALKMGDIFILDADCELGN
                     QDHVGLDYKALPQEVSAGDVLLLDDGRITMQVDRVKGNQIHCLVLNGGVLSNNKGINR
                     QGGGLSADALTKKDREDIKTAVKLEADYIAMSFPKNALDVELARTLVKKAGGNSSIIA
                     KIERAEAIENLEAIIESSDAIMVARGDLGVEVGDAAVPGLQKRMIRMARAQNKLVITA
                     TQMMESMITSPIPTRAEVSDVANAVLDGTDAVMLSAETAAGQYPLETVQAVHRVVMEA
                     EKEYINQYHAQKSDQVFMKTDEAVAAAAIYTAKHLKVKAIAALTQSGNAAQWLSRADA
                     EVPIYALSPDKFARRKLTLHRGVYPFPIEQGSKKRDEVLNEMEQVLLKERAVSTGDLV
                     LFTFGEPIGAPGGTNTLKIVKIGEQKN"
     gene            complement(4409..5590)
                     /locus_tag="PKL42_06990"
     CDS             complement(4409..5590)
                     /locus_tag="PKL42_06990"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNU66861.1"
                     /translation="MAVIKMTDLDLSGKRVLIRADLNVPVADGKVTSDARITASMATI
                     EHALKAGAKVMVTSHLGRPEEGVYSEENSLKPVVDVISTKLGKPVRLIKDWLEGGFDV
                     AAGELVVLENCRFNKGEKKSTDELSQKYAKLCDVFVMDAFGTAHRAEASTHGVAKFAP
                     VAAAGILLVNELDALTKALLSPARPMVAIVGGSKVSTKLTVLESLSEKVDQLVVGGGI
                     ANTFLKAAGNEVGKSLCEDDLVPVARTLMDKMSQRGAAVPIAVDVVCGKKFDANEPAI
                     KKDVAAVAADDMIFDIGAKSASQIAEIIKNAGTVVWNGPVGVFEFDQFGEGTKTIANA
                     IATTKAFTLAGGGDTIAAIQKYGIEDKVDYISTAGGAFLEFLEGKQLPAVAILEQRAK
                     R"
     gene            complement(5607..6626)
                     /gene="gap"
                     /locus_tag="PKL42_06995"
     CDS             complement(5607..6626)
                     /gene="gap"
                     /locus_tag="PKL42_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNU66862.1"
                     /translation="MAIRVAINGYGRIGRMVLRALYESGRKDIEIIAINSADCDAESN
                     AHLTRYDSAHGRFPFDVGVDGDDMIIGSQKIKTFASRNPAELPWRDLAIDIVLECTGA
                     FNSKAKAQAHLDAGAKKVLLSAPGDKDIDATVVYGVNHHVIKASHTVISNASCTTNGL
                     APMVQPLHEKIGIVNGLMNTIHSYTNDQVLTDSHHADMRRARSATTSLIPTKTGAAAA
                     VGLVLPELNGKLDGFAIRVPTINVSLVDLTFTASRPTTKEEVNHIMHLAAGSGALKGI
                     LSYNEAPLVSVDYNHTNFSSNFDATLTKVTKDGLLVKVCAWYDNEWGFSNRMLDNAVA
                     MMAAK"
     gene            complement(6761..>7812)
                     /locus_tag="PKL42_07000"
     CDS             complement(6761..>7812)
                     /locus_tag="PKL42_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNU66863.1"
                     /translation="YAKAFPAEAAEYTRRMAGDLPANWKAETDKIIAAVNEKAEGNAS
                     RKASQNAIGALAPLLPEFLGGSADLTGSNLTSCGSFKHVDAKNPGNYISYGVREFGMA
                     AIMNGMALHGGLLPFGGTFHMFSDYMKSGMRMSALMHQRVIYVLTHDSIGQGEDGPTH
                     QPVENTSGLRYIPRMDVWRPADATETTVAWVAAVERKDGPSSLVLSRQNLAGVKHDAK
                     DFDLIRKGGYVFSDAAGKADVIIIANGSELDLAVKAAAELTAAGTKVRVVSMPSTNVF
                     DRQDQAYKDSVLTLGVKRVAVEAAHPDFWRKYVGLEGAVVGINTFGESAPGGALMKHF
                     GFTVENVVATVKSVL"
BASE COUNT         2086 a         1980 c         1571 g         2175 t
ORIGIN      
        1 gccagaacat gagcttgaag aacttaccgg tatttatgtg aaacgaggat taacgtcaga
       61 cctcgccaag caagtggcgc ttcaactaac cgcacatgat gcacttggtg cgcacgcacg
      121 ggacgaattg ggcattattg aagcaacgaa tgctaaacct gtacaagctg cactggcctc
      181 agcgggtacg tttgcagttg gggcgctgtt gccgttactc gtggcctggt ttgcatcaga
      241 aactaaactg gtattaagtg tcgcggtcat gtcattagta tttttagcgt tacttggcgg
      301 attggctgca aaagcgggtg gcgcaagcat ttggcagggt accgcccgtg ttaccttttg
      361 gggtgcctta gccatgctgg ccaccgccgg tgtggggagc ttgtttggtg tggctatcgc
      421 ataacggcaa aagcgctgac atgatgttgc accaacaaaa aaagcgtgga aacccacgct
      481 ttttttataa tatttgagct gcgcgttcat tcctataacg ctgacaagcc ctaagactgg
      541 tcgcggttag aaagctggtt ttacttctgc gttattgtaa cgcgccacac cgtccataat
      601 ttccttgtgc gcgtcgtcaa ttcccaccca gccttcaatt ttcacccatt ttcctttgtc
      661 caaatctttg taatgctcaa agaagtgaga gatcatttct aagcgccagg gtgaaacgtc
      721 tttataggtt ttttggtagc catataaacc gcaaactttt tcaacaggca ctgcgagtaa
      781 ttttgcatca ccccctgctt catccgtcat ttgcaacatg cctaacgcgc ggcaacgcac
      841 caccacacct ggcaacaatg gcacaggggt aattactagc acgtctaccg gatcaccgtc
      901 atcggccaaa gtatgcggaa tatagccgta gttacaagga tattgcatag acgttcccat
      961 gaagcggtca acaaaaattg cgccgctttc tttatccact tcatatttca cagggtcgcc
     1021 ctgcataggg atttcaataa tcacgttaaa atcgtgtggt aaatttttgc ctgatggaac
     1081 ttgattgagt gacattgcat atttccaata attaaataga gattcgtttg taaattcaag
     1141 gcgcgcattg taccaaagtc atgtaaataa attgtgacct gatacaaaaa ttaatatcaa
     1201 ctaacctgcg ctcggcattg ttgaatgatt tcttgagggg tgagctttaa cgcaagataa
     1261 aacaaagcgg gaataatcac cacatcatct agctgcccaa taataggaat aaaatcagga
     1321 atcaaatcaa agggtaacag caaatatcct accgccaagc ccaataataa cttcgccatc
     1381 cacggcgtct ctgggtgttt aagcaccaac cggtagactt ctagctcttt ttttaaatgg
     1441 cgataaaggg ttttgaactt atcaaacatc caacttttta ccagtaaggc gttcaaacgc
     1501 ttcaagatat ttttcggatg tgcgctgcgc tacgtcagcc ggcaatgcag gtgctggcgg
     1561 tgttttattc caacccgttg attccagcca atctctcaca tattgcttat caaagctcgg
     1621 cggatttttg ccaaccacgt agccatccgc aggccagaaa cgggatgaat caggcgtgag
     1681 tatttcgtca atcaaataca acgtccccgc atcatcaaca ccaaattcaa atttagtatc
     1741 ggcaatgatg atgccgcgcg taagcgcata ctgcgcagcc tcggtgtaga gttgaacact
     1801 cactttagcc actttttcag ccaattcttt gcctaacaac gttgcgcatt tttcgagcga
     1861 aatgttttcg tcgtggtcgc ctatttccgc cttgcttgaa ggagtaaaga gtggctgtgg
     1921 taactgcgag gcttcttgca aatttgcagg aagcgcaata ccgcataccg tgcctttagc
     1981 ctggtactct ttccaaccac tgccaacgag gtagccgcgg acaatggctt caataggtaa
     2041 aggctttaat tttttggtga caatggcgcg gccatgtact tgcgattttt catccgcact
     2101 aaccacggtg gcagggtcaa tgcccgtgag gtggttcggt acaatatgct ttaatttatc
     2161 aaaccaaaaa ttggcaattt gtgtgagtac cgcccctttg ctggcaattg gtgtgggtaa
     2221 aatcacatca aatgccgata aacggtctgt cgttatcaat aataattgat tcgcatcaac
     2281 ggcataaata tcgcgcactt tgccttggtt tagtttatgt aaacttgtaa tgttgctata
     2341 taacaatgga gaactcaaag catcacctat taaatattat tttggaacat tggggttatc
     2401 accatcttca gtatatggaa aacgcacgtg gccgaatcgc acggcgagta ctgcaagcgc
     2461 gataatcaat accgctgtcg caacggctaa aatatgctcc ggtgtgagtg ttttaacttc
     2521 cagcaccaaa tagcgcgcca gtgccacaat accaatataa agcggatagc gtacgggcaa
     2581 ctttccggaa cccaaatatt gcttcaccat cgacagcact tcaaggtaca agaataaaag
     2641 tagcaagtcg ccaacaccaa ccttcgatgt cacccacatt ttatagattt catcaaatgt
     2701 ggcatagaca gttgccatac caatcaccac aagtacaagc tgctcgacat atttaatcgc
     2761 ctcttgcccg gcttttccta tttgactgct cgttttcaca aagtcatcct ttatctcaat
     2821 gagtacaatt ataactgacc gtaatcacct atattttaat ttttttgctc accaattttt
     2881 acgatcttta gcgtattagt gccgcccggt gcgcctatag gctcaccaaa agtaaataac
     2941 accaagtcac ccgtactcac agctctctct tttaataaca cttgctccat ttcgttcagc
     3001 acttcatccc gttttttact gccttgctca atgggaaaag ggtacacacc acggtgcaac
     3061 gtcaattttc tacgtgcaaa tttatcaggc gacaatgcat atatcggtac ttctgcatca
     3121 gcgcgagata accattgtgc cgcgttaccc gattgcgtca acgctgcaat ggctttcact
     3181 tttaagtgtt ttgcagtata gattgccgcc gccgcaaccg cttcatccgt tttcataaac
     3241 acttgatctg acttctgcgc atgatattga ttaatatact ctttttcagc ctccatcacc
     3301 acacgatgca cggcttgtac agtttctaac gggtattgcc ctgcggctgt ttcagccgaa
     3361 agcatgacag catctgtacc atccaacacc gcatttgcaa catcagacac ttccgcacgc
     3421 gttggaattg ggctggtaat catggactcc atcatttgcg tggccgtaat cactaattta
     3481 ttttgtgcgc gcgccatacg tatcatccgt ttttgcaatc ccggtacggc ggcatctccc
     3541 acctctacac ccaaatcgcc tcgggcaacc ataatggcgt cggatgattc aataatcgcc
     3601 tccagatttt caatcgcctc agcccgctca attttggcaa taatgctgga atttccgccg
     3661 gcttttttta ccaaagttct tgccaattca acatcaaggg cattttttgg aaatgacatc
     3721 gcaatataat ctgcttctaa ctttacggcg gttttaatat cctcacggtc ttttttagtt
     3781 aaagcgtcag ctgacaagcc gccgccctgg cgattgatac ccttattatt actcagcaca
     3841 ccgccattga gtaccaaaca atgaatttga ttccctttaa cccgatcgac ttgcatggtg
     3901 atgcgaccat cgtccaaaag caacacatcg ccagccgata cttcttgtgg caacgcctta
     3961 tagtctaggc ccacatgatc ttgattaccc agctcacaat cagcatcaag aataaatata
     4021 tcccccatct tcaaggcaac ttttcctagc tcaaacttgc cgatccgaat ttttggtccc
     4081 tgcaaatcac acaacacacc taccgggcgc cccaactttg ccgcaatgct gcgtacaatt
     4141 tcagcacgct taatatgttc ctcggctgtg ccatgagaaa agttaagccg caccacgtta
     4201 acgcctgcgg taatcatacg tgcaagcatt tcttcgctag aagaagatgg gcctaatgtt
     4261 gctacaattt tcgtttttcg taatgtcaaa tttttacctt atttctgtag cgtaaatgta
     4321 atgtatttta aatatcgtta gccaccatgt cgccaaacaa aaatggggcg aatagcccca
     4381 tttttaaagt gctaaacgta tcgggctact acctttttgc gcgttgctct aatatggcga
     4441 ctgcaggtaa ttgctttcct tccaagaact ccaaaaatgc cccaccagca gttgaaatgt
     4501 agtctacttt atcttcaata ccatatttct gaatggctgc aatcgtgtct ccgccacctg
     4561 ccaaagtaaa ggccttagta gtggcaatcg cattcgcaat cgttttggta ccttcaccga
     4621 actggtcaaa ttcaaacaca ccaacgggcc cgttccaaac cacagtccca gcatttttaa
     4681 taatctccgc tatttgacta gcagatttcg cgccaatatc aaaaatcata tcgtcagcag
     4741 ccaccgcagc cacgtctttc ttgatagctg gctcatttgc atcaaatttt ttgccgcaga
     4801 ccacatctac cgcaataggt actgctgcac ctctttggct cattttatcc attaaagtgc
     4861 gcgccacggg aacgagatca tcttcacaca aagatttacc aacttcattt ccagcggctt
     4921 ttaagaacgt attggcaata ccgccaccca ccaccagctg gtcgactttt tcagacaaac
     4981 tttcaagcac agtcaacttg gtagaaactt tactaccgcc cacaattgct accattgggc
     5041 gtgccggact aagaagtgct tttgtgagcg catctaattc attcaccaac agaatacccg
     5101 ctgctgcaac aggtgcgaat ttagctacac catgcgttga agcctcagcg cgatgtgccg
     5161 taccaaaagc atccatcaca aacacatcac acagtttggc atacttttgt gacaattcat
     5221 cagtgctttt tttctcacct ttattaaaac ggcaattctc caaaacgacc aattctccag
     5281 ccgctacatc aaagccacct tcaagccaat ctttgattaa ccgtaccggt ttcccaagtt
     5341 tggtagaaat tacatcaacc acgggcttga gagaattttc ttctgaataa acgccttctt
     5401 ctggacgacc caaatgggaa gtcaccatga cttttgcgcc agctttgagc gcgtgttcaa
     5461 tcgttgccat ggatgcggtg atgcgggcat cagaggttac ttttccatca gccactggca
     5521 catttaaatc tgcacgaatc agcacacgct ttccgcttaa atccaaatcg gtcattttga
     5581 ttactgccat cacatcactc ctttatttat tttgcagcca tcatcgccac agcattatcc
     5641 agcatacggt tactgaaacc ccactcattg tcataccatg cacaaacttt aacgagcagc
     5701 ccatccttgg ttacttttgt gagggtggca tcaaaatttg atgagaaatt ggtatggttg
     5761 taatccactg aaactaatgg cgcctcgttg taagacaaaa taccttttag cgcaccactg
     5821 ccagcggcta agtgcatgat gtggtttact tcttctttag tggttgggcg tgatgcggta
     5881 aaggttaaat ccaccaaaga aacattaatg gtcggaaccc gaatcgcaaa accgtctagc
     5941 ttaccgttta actctggcaa taccaaaccc acggcggcgg cggcgccagt tttggtaggg
     6001 ataagcgaag tcgttgcaga acgtgcccga cgcatatccg catggtgcga atcagtgagt
     6061 acttggtcgt tcgtgtaaga gtgaatggta ttcattaagc cattcacaat accaattttt
     6121 tcatgcaaag gttgcaccat tggcgccaac ccattggtgg tgcaggatgc atttgaaatg
     6181 acggtatggc tggctttaat cacatgatga ttcacgccat acaccaccgt tgcatcaata
     6241 tctttatcac ccggcgctga taacaatact ttttttgcgc ctgcatcaag atgcgcttgc
     6301 gctttagcct tactgttaaa tgcgcccgtg cattccaaca caatatcaat ggctaaatca
     6361 cgccaaggca gttcagcagg gttacgagaa gcaaaagttt taattttttg gctgccgata
     6421 atcatatcgt caccatccac acctacatca aacggaaagc ggccatgcgc actatcataa
     6481 cgcgttaaat gtgcattgga ttctgcatca caatcggcac tgttgatggc gataatttcg
     6541 atgtcttttc gcccagactc atatagcgct cgtaacacca tgcgcccaat gcgcccatat
     6601 ccattaattg ctactcggat tgccatgaaa attcctaaat taaacttttt acctaattaa
     6661 aagaaataag gcacccctaa tgaggtgcct tactaaactt acgcactttc agccctaggg
     6721 ccgaattaag tgcgcattag tcaaactgca acaccttgaa ctacaggact gattttacag
     6781 ttgccaccac attttcaacc gtaaagccga aatgtttcat caacgcgccg cctggtgctg
     6841 actcgccaaa ggtattaata ccaacaaccg cgccttctaa acctacatat ttacgccaga
     6901 aatcaggatg cgccgcttct acagccacac gtttaacgcc tagggtcaat acgctgtctt
     6961 tataggcttg gtcttgacga tcaaatacgt tggtagatgg cattgaaacc acgcgaactt
     7021 tagtacctgc agcagttaat tctgccgccg ctttcactgc taaatctaat tcagagccat
     7081 tggcaatgat aatcacatcg gctttaccgg cagcgtctga gaatacataa ccacccttac
     7141 ggattaaatc aaaatctttg gcatcgtgct taacacccgc caagttttga cgtgaaagta
     7201 ccaagctgct tggaccatct ttacgttcaa cggctgcaac ccatgccact gtcgtttcag
     7261 ttgcatcagc tggacgccat acatccatac gaggaatgta acgtaagcca gaagtatttt
     7321 ctactggttg atgggttgga ccatcttcac cttggccgat agagtcatgt gttaatacat
     7381 aaatcacgcg ttgatgcatc aatgcagaca tacgcatgcc gctcttcatg tagtctgaga
     7441 acatgtggaa tgtgccgccg aatggtagta aaccaccgtg caatgccata ccgttcataa
     7501 tggctgccat accaaattca cgcacaccgt aagagatgta gtttcctggg tttttagcat
     7561 ctacgtgttt aaagctaccg catgaggtta agttagaacc tgttaagtcc gcagaaccgc
     7621 ctaagaattc tggcaataat ggtgctaatg cgccgattgc attttgtgat gctttgcggc
     7681 ttgcattgcc ttccgctttt tcgttcactg cggcgataat tttatctgtt tctgctttcc
     7741 agttagctgg caaatcacct gccatgcggc gtgtgtattc agccgcttct gctgggaatg
     7801 cttttgcata gg
//
LOCUS       DAOQSI010000096         7800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_8950_length_7800_cov_5.943835, whole genome shotgun sequence.
ACCESSION   DAOQSI010000096 DAOQSI010000000
VERSION     DAOQSI010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 7800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7800
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8950_length_7800_cov_5.943835"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PKL42_07005"
     CDS             complement(<1..384)
                     /locus_tag="PKL42_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNU66864.1"
                     /translation="MNTKNKALPLLGGITPKAFLAEYWHKKPLLIKNAIADFTGLLSP
                     EELAGLACEDEVQSRIVEEINGVWHAKHGPFDENDFAALPDKPNPNHRWTLLVQSVNH
                     YLPEASALLQQFDFIPHARLDDLMVS"
     gene            522..2657
                     /locus_tag="PKL42_07010"
     CDS             522..2657
                     /locus_tag="PKL42_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HNU66865.1"
                     /translation="MNAPAETSIKTFHGGCPHDCPDTCSMVYTVKDKKLISVTGNKEH
                     PMTRGGLCVKLKDYEKRHYHPDRLLYPLKRTGPKGSKQFKRITWDEALDEITTKWKAI
                     IAKDGPHAIMPNSYLGNQGLVHGLNGGDAFFNRMGATVCERTFCGEGSCTAWLLTVGP
                     TAGVDPESFIHANYIVIWACNSVSTNLHHWHIIHEAQKKGAKVVVIDSYASKTAKEAD
                     WHIAPKPGTDGALAMAMMNVIIAEDLVDQDYIDNYTIGYKELAERAKTRTPEWAAEIT
                     GIAADDIRKLAREYATTPPAAIRMGVALERSYGGSQAIRAVSSLPALIGAWRHVGGGA
                     LQFPVWEHPYKFDIISRPDLIPEGTPVVNNLQLGRALTGELNLKTPIKSMMVWNTNPL
                     TQAPETDKIAKGLANEDLFLVVAEHFMSDTAAYADIVLPAAMGAEMEDMVLSWGHLYL
                     TYNEKCIDPPGEAIPNNEIFRQLAKRLGYEEDNFKWNDTECLENYVDWNAPTCEGIDL
                     AYLRKHGFARLKAFGDKDVRARHANGDFPTPTGKCHFLVEGAKNFVAGPFRQMYEGFQ
                     PGEDLDSLPDYVPSRETPATNPTLAAKYPLNIISPKSHGFLNSCYANVEEKIKGQGEQ
                     FVLINQFDATARGINTGSVVKVFNDRGTFEGVAKISDDVNAGIIVATLGYWRQLNNGT
                     VNCISSAEFGDMGHSTTFSDNLVEVALAK"
     gene            2873..3889
                     /locus_tag="PKL42_07015"
     CDS             2873..3889
                     /locus_tag="PKL42_07015"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HNU66866.1"
                     /translation="MNIYKLISPLLFQFDAETVHDFTLNSLKTAERLGALKFYPAPPV
                     CQPRQVMGITFPNAVGLAAGLDKNAAVIDGMAALGFGFIEVGTVTPRPQPGNPKPRLF
                     RVEEAQGIVNRFGFNNLGVDHLIENVKAAKYKGVLGINIGKNFDTPNENAVDDYLVCM
                     RKVYAYASYITVNISSPNTKNLRALQEKEALSHLLATLKLEQLKLAGTHGRYVPVALK
                     IAPDLAQSQVNEIADLLMQHNIDGVIATNTTLARDMVQGMAHAEETGGLSGAPVREKS
                     TLVIQQLSQRLQGAVPIIGVGGILSGADAVEKIAAGADLVQVYSGLIYKGPKLVHEVC
                     SSLG"
     gene            3902..4576
                     /locus_tag="PKL42_07020"
     CDS             3902..4576
                     /locus_tag="PKL42_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HNU66867.1"
                     /translation="MALPILYSYRRCPYAMRARMALKLANVYVEIREISLREKPAHML
                     QVSPKGTVPVLVLPDGKVIDESLEIMQWALKTHALQPQGLSSQNIPAKAGHSCVNANI
                     HVGAGASVLALIAENDGEFKRALDAYKYPERYPHKTQIQYRADGEVFLQKLENLLQEN
                     QYLFGNTFSMADIAIFPFVRQFAEVDAAWFEVTPYVQLRIWLRTLVESALFLSIMEKR
                     PTHINM"
     gene            complement(4612..6885)
                     /gene="clpA"
                     /locus_tag="PKL42_07025"
     CDS             complement(4612..6885)
                     /gene="clpA"
                     /locus_tag="PKL42_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HNU66868.1"
                     /translation="MIAQELEVSLHMAFMEARQKRHELITVEHLLLAMIDNPTAAEVL
                     RACGATLETLRTELSQYIEEHTPTVGGQEDVDTQPTLGFQRVIQRAMLHVQSSGKKEV
                     TGANVLVAIFGEKDSHAVFFLHQQGITRLDIVNYISHGVSKIPDTEKPESADQEVETE
                     APPAGALENYTLNLNVQVAAGKIDPLIGRGPELERVVQTLCRRRKNNPLLVGEAGVGK
                     TAIAEGLARRIVEGDIPEVLANATVYSLDMGALLAGTKYRGDFEQRLKAVMKQLAEKP
                     DAILFIDEIHTLIGAGSASGGTLDASNLLKPALSNGSLKCIGATTYQEYRGIFEKDHA
                     LSRRFQKVDVTEPSVAETIEILKGLKSRFEEHHHVKYSASALTTAAELSAKYINDRHL
                     PDKAIDVIDEAGAAQRILPKSKQKKVIGNKEIEDIIAKIARIPPKNITADDRSALKTL
                     ERDLKAVVFGQDKAIEALTSSVKMARSGLGGNNKPVGSFLFSGPTGVGKTEVAKQLAY
                     IMGIELIRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLMTEAVTKHPYCVLLLDEIEK
                     AHPDIFNILLQVMDHGTLTDNNGRKADFRNVTIIMTTNAGAESLSKSSMGFTTSKQVG
                     DEQADIKRLFSPEFRNRLDATVSFAPLSQEIIIRVVDKFLIQLEDQLHDKKVEVTFSD
                     ALKAFLGKKGFDPQMGARPMARLIQDTIRKALADELLFGKLANGGSVHVDIDSKDEVK
                     LVFADDKTLSESVLVEA"
     gene            complement(6931..7242)
                     /gene="clpS"
                     /locus_tag="PKL42_07030"
     CDS             complement(6931..7242)
                     /gene="clpS"
                     /locus_tag="PKL42_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HNU66869.1"
                     /translation="MANAPLDANTVLKPEVAKPKLPPMFKVLLLNDDYTPMEFVVECL
                     ERFFNKNREQATQIMLKVHTEGVGVCGIYPQDIAETKMNQVLTLAKEHQHPLQCVIEP
                     T"
     gene            complement(7343..7762)
                     /locus_tag="PKL42_07035"
                     /pseudo
     CDS             complement(7343..7762)
                     /locus_tag="PKL42_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
BASE COUNT         2058 a         1692 c         1836 g         2214 t
ORIGIN      
        1 actcaccatc aagtcatcca gccttgcgtg aggaataaaa tcaaactgct gcaataaggc
       61 gctggcttca ggcaaataat ggttgacgct ttgcaccagc aatgtccagc gatgattggg
      121 attgggtttg tcaggcagtg cggcaaaatc gttttcatca aacggaccat gcttggcatg
      181 ccaaacacca ttgatttctt ccacaatgcg cgattgtact tcgtcttcac acgccaaccc
      241 tgctagttct tctgggctga gtaagccggt gaaatcggca atcgcatttt taatcagtaa
      301 cggttttttg tgccagtatt cagccaaaaa tgctttgggt gtgatgccgc cgaggagtgg
      361 caaagcttta ttttttgtgt tcatttcgtt caaattggat tgagttttct gagtgattat
      421 tatgccattg cattgctata caggttggta tatattgact gaaaaaataa gaatatgtgt
      481 catctacatt tgtatgtaag ttgatttttg aggagagaac gatgaatgca ccagctgaaa
      541 cttcaataaa aacctttcat ggtggttgtc cacatgattg cccggatact tgcagtatgg
      601 tgtataccgt taaagataaa aaactgattt cagtcactgg caataaagag catcccatga
      661 cgcgtggcgg cctatgcgtc aagctcaaag attacgaaaa acgccactat cacccagacc
      721 gcttactcta tccgctcaaa cgcacaggcc ccaaaggcag taaacagttt aaacgtatta
      781 cctgggatga ggcactagat gaaataacta ctaagtggaa ggcgattatt gccaaggatg
      841 gcccgcacgc gataatgcct aatagctatt taggcaacca aggcttggta catggcctta
      901 atgggggtga tgcatttttt aaccgcatgg gggcaactgt atgtgaacga actttttgtg
      961 gagaaggctc atgtaccgct tggttattaa ccgttggacc aaccgcgggg gtagatccag
     1021 aaagttttat acatgctaac tacattgtga tttgggcatg taattcagtc agtacaaatt
     1081 tacatcactg gcacatcatt cacgaagcgc agaaaaaagg tgctaaggtg gtggtgattg
     1141 actcctatgc gtctaaaact gcaaaagagg ccgattggca tattgcccca aaacccggca
     1201 ctgatggcgc acttgcgatg gcgatgatga atgtgattat tgcagaagac ttggttgacc
     1261 aagattacat tgataattac accataggct acaaagaatt ggcagaacgt gctaaaacgc
     1321 gcacccctga atgggccgct gaaatcacag ggattgctgc agatgacatt cgaaaacttg
     1381 cccgtgaata tgccactacg ccaccagcgg cgattcgcat gggtgtcgcg cttgaaagaa
     1441 gttatggcgg tagccaagca atacgcgcag taagcagttt acctgcattg attggtgcat
     1501 ggcgccatgt gggtggcggt gcgttgcagt ttccggtgtg ggagcatcct tacaaatttg
     1561 atatcatttc gcgcccagat ttaattccgg aaggcacacc agtggtaaat aatctgcaat
     1621 tagggcgtgc gcttacaggt gagcttaatc ttaaaacacc gattaaatcg atgatggtat
     1681 ggaacactaa tccactcact caagcaccag aaactgacaa aattgccaaa ggcttagcga
     1741 atgaagattt gtttttagtt gtggctgagc attttatgtc agacacggca gcctatgccg
     1801 atattgtact gcccgcggcc atgggtgctg aaatggaaga tatggtgctg tcatggggac
     1861 atttgtacct tacctacaat gaaaaatgta ttgacccacc aggtgaggct attcctaata
     1921 atgaaatttt taggcaatta gccaaacgtt tgggctatga agaagacaac tttaagtgga
     1981 atgacaccga gtgccttgaa aattacgttg attggaatgc accaacttgt gagggcatcg
     2041 atttagcgta tttacgcaaa catggctttg cgcgcttaaa agcttttggc gataaagacg
     2101 tacgcgcccg ccatgccaac ggagattttc ctactccaac gggtaaatgt cactttttgg
     2161 tagaaggagc aaaaaacttt gtcgcagggc ctttccggca aatgtatgaa ggttttcagc
     2221 caggtgaaga tttggacagc ttgcctgatt acgtgccatc acgcgagaca ccagcgacta
     2281 acccaacttt agcagcaaaa tacccgttaa atattatttc gcctaaaagt catggctttt
     2341 taaattcttg ttatgccaac gtggaggaga aaattaaagg gcagggtgag cagtttgtgc
     2401 tgattaacca gtttgatgcc acagcacgtg gcattaatac aggcagtgtc gtgaaagtgt
     2461 ttaatgatag aggtacgttt gaaggggtgg caaaaatctc agacgatgta aatgcaggga
     2521 ttattgtcgc cacacttggt tattggcgcc agttaaataa tggcacggta aattgcatta
     2581 gctctgcaga gtttggtgat atggggcatt ccacaacatt tagtgacaat ttggtggaag
     2641 tggcgctggc aaaataaact atgaatgaat ggttaattta gtcaaaattc aaccacaacc
     2701 ttttttgatg ctctggaagc cttgtaaata aaggcttcca gagcattatt tttttcacgt
     2761 agaattttga gtgtttttat tcggtttttt agtgcaattt tggcagcggt tttttgccta
     2821 cacgcgcgct gtgatttttg gtgatattgc agaatacaag taaaattgca gcatgaatat
     2881 ttacaaactt atcagcccac ttttgtttca gtttgatgct gaaactgttc acgactttac
     2941 cttaaacagc ttaaaaactg ctgaacgttt gggcgctttg aagttttacc ctgcgccacc
     3001 tgtttgtcag cctcgacagg tgatggggat tacttttccg aatgcggtgg gtttagccgc
     3061 gggcttggat aaaaacgcag cggtgattga tggaatggct gcgttaggtt ttggttttat
     3121 agaggttggc acagtgacgc cccgtccgca gccgggcaat cctaaaccga gattgtttcg
     3181 tgtggaagaa gcacaaggca tcgtgaaccg ttttggcttt aataacttag gtgtggatca
     3241 cttaattgaa aacgtgaaag ctgccaaata taaaggcgtg ctgggcatta acattggcaa
     3301 gaattttgac acgccaaatg aaaatgcagt ggatgattat ttagtttgca tgcgtaaagt
     3361 gtatgcgtat gcgagttata tcacggtgaa tatttcctca cccaacacca agaatttgcg
     3421 tgcattgcaa gaaaaagaag cgttgtctca tttactcgcc acgctcaagc ttgagcagtt
     3481 gaaactggcc ggcactcacg gacgttatgt gccagtggct ttaaaaatcg cgccagactt
     3541 agcgcagagc caagtgaatg aaattgctga tttgttgatg caacataaca ttgacggtgt
     3601 aattgcgacc aatacgacgt tggcgcgcga catggtgcaa ggcatggcgc atgccgaaga
     3661 aacaggcggt ttgtcaggtg cgcctgtgcg tgaaaaatcg acattagtca ttcagcaatt
     3721 gtcgcaacgc ttgcagggtg ctgtgccgat tatcggcgtg ggtggtatat taagcggtgc
     3781 agatgcagta gaaaaaatcg ccgcaggcgc agaccttgtg caggtttaca gtgggttaat
     3841 ttacaagggg cctaaactgg tgcacgaagt ttgtagctcg ctaggctagc ttttagctgt
     3901 catggcacta ccaattctat attcatatcg tcgctgccct tacgcgatgc gtgcgcgtat
     3961 ggcgcttaag ttggcaaacg tttacgttga gattcgcgaa atttcactac gcgaaaaacc
     4021 agcccatatg ttgcaagttt cccccaaagg caccgtgcct gtgttggtgc tacctgatgg
     4081 caaagtaatt gatgaaagct tagaaatcat gcaatgggca ttaaaaacgc atgccctgca
     4141 accacaaggg ttgtcctcgc agaatatacc agctaaagcg ggtcattcat gcgtgaacgc
     4201 caatattcat gtgggtgctg gagcatctgt tttagcgctt attgcagaga atgatggcga
     4261 gtttaaacgc gcgttagatg cttacaaata tcctgagcgt tacccacata aaacacaaat
     4321 tcaatatcgg gccgatggcg aagtgttttt gcaaaaacta gaaaacctac tgcaagaaaa
     4381 tcaatattta tttggcaata cgttcagtat ggctgatatt gcaatctttc catttgtgcg
     4441 gcagtttgct gaggtagatg ccgcttggtt tgaagttacg ccatatgttc aattgcggat
     4501 ttggctaaga acactggttg aatctgcgtt atttttgagc atcatggaga aacgaccaac
     4561 gcatatcaac atgtaggcgc gatttttaaa tcgcgcctac aataggatga attaagcttc
     4621 tactaggacg ctttctgaaa gtgttttatc gtccgcaaaa accaatttca cttcgtcttt
     4681 gctatcaata tccacatgca cgctaccacc gttggcaagt tttccgaata acaactcatc
     4741 tgccagcgct ttgcgaatag tgtcttgaat caagcgcgcc attgggcgag cacccatttg
     4801 tgggtcgaaa ccttttttgc ccaaaaaggc ttttaaggca tcgctgaagg tcacttccac
     4861 ttttttgtca tgcaattgat cttctagttg aatcaagaat ttgtctacca cgcggatgat
     4921 aatttcttgt gataatggcg caaatgatac agtcgcatcc aagcggttgc ggaactccgg
     4981 tgagaataag cgtttaatat cggcctgctc atcgcccact tgtttgcttg tggtaaagcc
     5041 catgctggat ttagataagc tttcagcacc agcgttagtc gtcatgataa tcgtcacgtt
     5101 tctaaaatct gcttttcggc cgttattgtc tgtgagcgtg ccgtgatcca tcacctgcaa
     5161 cagaatattg aaaatatccg gatgggcttt ttcaatttcg tccaacaaca atacgcaata
     5221 cggatgcttc gttacggctt cagtcatcag gccgccttgc tcaaaaccca catagcccgg
     5281 aggcgcacca atcaaacgcg agaccgcatg gcgctccatg tattcactca tgtcaaagcg
     5341 gatgagttcg atgcccatga tataggccag ctgttttgca acttcggttt taccaacacc
     5401 ggtcgggccg ctgaataaaa agctgccgac aggcttattg ttgccgccca agccgctacg
     5461 cgccattttt acggaagatg tgagggcttc aatggcttta tcttggccaa acaccacggc
     5521 tttcaaatcg cgctccagcg ttttgagcgc acttctgtca tccgcagtaa tattttttgg
     5581 tggaatacgc gcaattttgg cgatgatatc ttcaatctct ttattgccga tgactttttt
     5641 ctgttttgat tttggcaaaa tacgttgtgc cgcaccggct tcatcaatca cgtcaatcgc
     5701 tttatcaggc aagtggcggt cgttaatgta tttagctgaa agttctgccg ctgtcgtgag
     5761 tgcagacgct gaatatttca catgatgatg ctcttcaaag cgtgatttca agcctttcaa
     5821 aatctcgatg gtttcagcca cactcggttc agtcacatcc actttttgaa agcggcgcga
     5881 tagcgcatgg tctttttcaa aaataccgcg atattcttgg tatgtcgttg cgccgatgca
     5941 ttttaacgag ccgtttgaaa gggctggctt gagtaagtta ctggcatcca aggtgccgcc
     6001 actggccgaa cccgcgccga ttaaagtgtg aatctcgtca atgaacaaaa tcgcatctgg
     6061 tttttcagcg agttgtttca ttacggcttt taagcgttgc tcaaagtcac cgcgatactt
     6121 cgtgccggca agtaaagcgc ccatatcaag tgagtaaacc gtggcgttcg ccagcacttc
     6181 tggaatgtcg ccttctacaa tgcgccgcgc taagccttca gcaatcgcgg ttttaccaac
     6241 acccgcttcg ccgactaaca acggattatt tttacggcga cggcataaag tttgcacgac
     6301 gcgctctaat tctgggccgc ggccaatcag cgggtcaatt tttccagcgg ccacttgcac
     6361 attgaggtta agcgtatagt tttctaaagc accggctggc ggcgcttcgg tttcaacttc
     6421 ttggtctgca ctttctggtt tttcagtatc aggtattttt gaaacgccgt gcgagatgta
     6481 attaacaata tctaaacgcg taatgccttg ttgatgtaag aaaaacaccg cgtgtgaatc
     6541 tttttcacca aaaatcgcga ccaatacgtt ggccccagtg acttcttttt tgcctgatga
     6601 ctgcacgtgc aacatggcgc gttgaatcac gcgttgaaaa cctaacgttg gttgtgtatc
     6661 aacatcttct tggccaccca cggttggggt atgctcttca atgtactggc tgagttccgt
     6721 ccgcagtgtt tcaagcgttg cgccacaagc gcgaagcact tcagccgcag tcgggttatc
     6781 aatcatggca agtagcaaat gctctaccgt gatgagttca tggcgttttt gtcttgcctc
     6841 cataaaggcc atgtgcaggc tcacttctaa ctcttgggca atcattttat ttcctaactt
     6901 tcaatatgta ttctgtaatt gctagcgcca ttaagttggc tcaatgacac actgcaaagg
     6961 atgctggtgc tctttagcta gtgtaagcac ttgattcatt ttggtttctg caatatcttg
     7021 cgggtaaata ccgcacacgc ctacgccctc tgtatgcact ttgagcataa tttgcgttgc
     7081 ttgttcacgg tttttattaa aaaaacgttc caagcactca acaacgaatt ccatgggcgt
     7141 gtaatcgtca ttcagtagca gtactttaaa catggggggc aattttggtt ttgcaacctc
     7201 aggcttcagt acggtatttg catcaagtgg agcatttgcc ataatcagaa tctattttgg
     7261 gctaaactgg aaaaaattca agcttaattt cgccgattta ttgaggatga tactacgcgt
     7321 ttgtacgatt caattgctaa gattaaggga atatcttcac gcttttcaca atacggtctt
     7381 gggtttgaat gatttcaagc acatgatcac ccattttaaa gctggtgttt gattccggaa
     7441 tgtcttcaaa atgttccaaa atcaggccgt ttaacgtgcg cggaccatcg agcggtagtt
     7501 gtaaatctaa ttttttgttc aagtcgcgca gcatactgct gccatctacc agccagccgc
     7561 catcttcatc ttggcgataa ctgcctgtgc ggcttggtga ctgcgtggtg aagtcgccaa
     7621 tcatttcctc taaaatatct tcgagcgtaa ctaagccttt aaattcgccg tattcatcta
     7681 cgaccagcgc tacacgctgc tggtgttctt gaaactgttg aatttgcgta taaagcggtg
     7741 tgcctgatgg tataaaataa ggcacaccgc tttatacgca aattcaacag tttcaagaac
//
LOCUS       DAOQSI010000097         7776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_8999_length_7776_cov_5.054527, whole genome shotgun sequence.
ACCESSION   DAOQSI010000097 DAOQSI010000000
VERSION     DAOQSI010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 7776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7776
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8999_length_7776_cov_5.054527"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /gene="ispG"
                     /locus_tag="PKL42_07040"
                     /gene_synonym="gcpE"
     CDS             <1..1099
                     /gene="ispG"
                     /locus_tag="PKL42_07040"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNU66870.1"
                     /translation="VNSPEAAAQVGNIRRGLDALGCNVPLIGDFHYNGHKLLAAYPDC
                     AQSLAKYRINPGNVGKGSKRDEQFAAMIETAVKYDKAVRIGVNWGSLDQAKMAQMMDD
                     NAKLMQPLSSDALMREALIQSALESAEQAAAIGLPKNKIIISCKVSNVQDLVKVYSEL
                     SSRSEYPLHLGLTEAGMGSKGIVASTAALALLLQQGIGNTIRVSLTPEPNESRTKEVV
                     VAQEILQTMGIRSFTPLVTACPGCGRTTSTYFQELAMQIQTYLRNQMPTWRSLYAGVE
                     NMNVAVMGCVVNGPGESKLANIGISLPGTGETPVAPVFVDGEKTVTLKGDNIAQEFQA
                     IVEDYVKKNYADGGRLHDPHFKKIVPIQAVK"
     gene            1152..2411
                     /gene="hisS"
                     /locus_tag="PKL42_07045"
     CDS             1152..2411
                     /gene="hisS"
                     /locus_tag="PKL42_07045"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNU66871.1"
                     /translation="MSHQFQSIKGFYDILPEATPLWFKLEDTARRVLAQYGYNNIRMP
                     LVENTDLFVRSVGEHTDIVEKEMYAWEDKLNGDKLTLRPEGTAGCVRAVVENNLTYNG
                     PVRLWYSGAMFRHENVQKGRQRQFHQIGVEAFGFASPEVDAEQIVLLARLWRELGIAD
                     VELQINSIGDAHERAEYRKVLIAYFEQHAEVLDEDAKRRLHTNPLRILDSKNPRMQAM
                     CEAAPKLIDCLGDETRAHFASLCQLLDDAGVAYTINARLVRGLDYYNRTVFEWVTTKL
                     GAQGTIAGGGRYDSLVERLGGNPTPACGFGIGLERVFLLMQEYGVIAEKAPDIYLVNV
                     GELAEQQAFKVAETLRGAGLSVVLHAGGGSFKSQMKKADRSGARYAAILGDDEVQANQ
                     ISLKPLLQSGEQVKCAVNQVASSILAK"
     gene            2606..3451
                     /locus_tag="PKL42_07050"
     CDS             2606..3451
                     /locus_tag="PKL42_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNU66872.1"
                     /translation="MTPLLQISNLTITPKKQPTRLLVNGVSFNIEAGKTTCVVGESGS
                     GKSLTALAVMGLLSKQLQLNSGSILFNNAQAGVQDLSKLDIASLQKIRGAKIAMIFQE
                     PMTSLNPVLTVGYQIGESLTAHLGLKGAALKAKVASLLEMVGIPAGRADSYPDELSGG
                     QRQRVMIAMSIACEPQILIADEPTTALDVTVQAQILQLLHELKTRMNMGMLFITHDFG
                     VVADIADNVVVMFRGEIVESGAKEAVLNNPQHPYTQALLACVPDAEGKKPLKPIDYSW
                     LGHAA"
     gene            3448..4248
                     /locus_tag="PKL42_07055"
     CDS             3448..4248
                     /locus_tag="PKL42_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNU66873.1"
                     /translation="MSELILEANHLVKTYTQRSGRFGFNTTLVKALDDVSVGLSAGKT
                     LAVVGESGSGKSTLARCLLQLHPFDSGEVIFQGQQLDQLNGQALRQMRQYLQMVFQDP
                     FASLNPRMRVGEIVGEGLLIHGLGSSSEQQKKVVAMLKRVGLTEADMQKYPHEFSGGQ
                     RQRVGIARALVLQPKVVVCDEPVSALDVSIQAQILLLLKELQAEMALSYLFISHDLRV
                     VRHIADDVAVMFQGRIVEQGSVADIYDNPREEYTRKLLSAIPGKATQH"
     gene            4336..4959
                     /locus_tag="PKL42_07060"
     CDS             4336..4959
                     /locus_tag="PKL42_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNU66874.1"
                     /translation="MAYNLEEQEQIDELKAWWKQYGKTVSSLVIGLVVAYAAYQIWNY
                     VQHKQAIEASTQYQELTVTDQKDLKAIQAKSATLMEQYSGTPYAARAALFAAKANYAA
                     NDTKSAKAQLDWAIKNAEETATRAMATLQLANILAEEKDYEGALKILDAPHDAGFDGL
                     FADLKGDVLMLQGKRADAKAAYELALSKLDAQGKYRVITQRKLEALG"
     gene            5006..6196
                     /gene="bamB"
                     /locus_tag="PKL42_07065"
     CDS             5006..6196
                     /gene="bamB"
                     /locus_tag="PKL42_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HNU66875.1"
                     /translation="MYLKSYQSVAYKTLGILALLVTTSCSTLTDLKTDISDRVFGREP
                     ADAPEALVEFKQTASSKVLWQAKIGPAEDFEFSPVIEAGFAYAAGASGELVKLDINSG
                     KQEWRVNAGEKLSGGVGVGGSLVLVGTSKGVVYAYDNAGKLQWKSKLSSEVLSAPRYF
                     DGIVIVRTNDNRIYGINAEDGSRKWVYDRTSPALTLRSSAGLVVDGGAVYAGFGGGKL
                     IALRADNGKLLWEASVAQPKGVTEIERIADITSLPVLDGPLVYAVAYQGRIAAIDRIS
                     GRVIWNRDISSLNGISVEDGRIFVPHSMGSVYALDYTTGKTFWRQAALKNRQLTAPVP
                     MGNYVAVGDLEGYLHFLTRDEGAFASRVKLAGSAVMPQMVLLNSTTVLAQTRDGGLYA
                     VQIK"
     gene            6326..>7776
                     /gene="der"
                     /locus_tag="PKL42_07070"
     CDS             6326..>7776
                     /gene="der"
                     /locus_tag="PKL42_07070"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNU66876.1"
                     /translation="MLPTVVLVGRPNVGKSTLFNRLTKSRDALVADLPGLTRDRHYGR
                     GLGGSQPYLVVDTGGFEPNTDSGILKAMATQTLLAIDEADVIIFIVDGRQGATPQDME
                     IANRLRRCKCPVLLAVNKTEGMNKAVVSAEFHELGLGDPLSISSAHGEGVKDIIELAL
                     EHFQVVEDAPENENSGDRVPKVAIVGRPNVGKSTLVNALLGEERVIAFDQPGTTRDSI
                     SIDLEKNGKHYTLIDTAGVRKRGRVLEAIEKFSVIKTIQSIEEANVVILVVDAQEGIT
                     EQDAHVAAYILDAGRALVVAINKWDGLKEEERDWIKREIDRKLMFLDFAEFHYISALR
                     KKGLPELFKSVDVAFKAAFAKLSTPQLTRVLIDALQQHQPPISKGIRPKLRYAHQGGS
                     NPPIVVIHGSHVDGVKDAYTRFLEKTFRRTFQLSGTPLRVQYKQGANPFAEDEDKRKS
                     GEGIVSMRRRKTAQRAELKAKKTAELEAGKAKKR"
BASE COUNT         2233 a         1462 c         2002 g         2079 t
ORIGIN      
        1 tgtcaattca ccagaagcgg ctgcacaggt gggcaatata cgccgagggc ttgatgcatt
       61 gggctgcaat gtgccgttaa ttggggattt tcattacaac gggcataagt tattggctgc
      121 ttaccctgat tgtgcgcaat cattagctaa atatcgtatt aaccctggca atgtgggcaa
      181 gggcagtaag cgcgatgagc agtttgccgc gatgatagaa acagcggtta agtacgataa
      241 agctgtgcgt atcggcgtga attggggcag tttagaccaa gctaaaatgg cgcagatgat
      301 ggatgacaat gccaaattga tgcaaccatt gtcgtctgac gcgctgatgc gtgaagcgtt
      361 aattcaatcg gcgttagaaa gtgctgaaca agctgcagca attggtttgc ctaaaaataa
      421 aataataatc tcttgtaaag tcagtaatgt acaagattta gttaaagtat attctgaact
      481 ttcaagccgc agcgaatacc cgctacattt aggtttaact gaagcaggga tgggttcaaa
      541 aggaattgtg gcttctacag cggcgttggc tctgttatta caacaaggta ttggcaacac
      601 catacgtgtg agcttaacac ctgaacccaa tgaatcgcgc accaaagaag tggttgtggc
      661 gcaagaaatt ttgcagacca tggggattcg ttcatttacg ccgctcgtga ccgcgtgccc
      721 aggctgcggt agaacaacgt caacgtattt tcaagagtta gccatgcaaa tacaaaccta
      781 cctccgcaat caaatgccaa cgtggcgtag cttatatgcg ggcgtagaaa atatgaacgt
      841 ggcggtgatg ggctgtgtcg tcaatggtcc gggtgaatct aaattggcca acattggcat
      901 tagtttgcct ggcacaggtg aaacacctgt tgcccctgtt tttgtcgatg gtgagaaaac
      961 cgtcaccctg aaaggggata atattgcaca ggaatttcag gcgattgttg aagattacgt
     1021 gaaaaaaaat tatgcagatg gcggcaggtt acatgatcca cattttaaaa aaatagtgcc
     1081 tatacaagcc gtaaaataaa cttcaaatta aaatttaatc atcactatat ttatcaagaa
     1141 tatttttttt catgagtcat caatttcaaa gcatcaaagg tttctacgac atactgcctg
     1201 aggctacgcc actttggttt aagttagaag acacagcaag acgtgttctt gctcaatatg
     1261 ggtacaacaa cattcgcatg ccgctggttg aaaataccga tctttttgtg cgcagtgtag
     1321 gtgagcatac cgatattgtt gaaaaagaaa tgtacgcttg ggaagataaa ctcaatggcg
     1381 acaaactcac gctgcgccca gaaggcactg caggttgcgt gcgtgccgtg gtggaaaata
     1441 atctgaccta caatggcccg gtgaggcttt ggtatagcgg tgcgatgttt cggcatgaaa
     1501 atgtgcaaaa aggtcgtcaa agacagtttc accaaattgg cgtggaagcg tttggctttg
     1561 cctcacctga agtggatgct gagcagattg ttttgttggc cagattgtgg cgcgagttag
     1621 gcattgccga cgtagaattg caaattaata gtattggcga tgcacatgag cgtgcagaat
     1681 atcgcaaagt gctaattgcc tattttgagc aacacgccga agttttagat gaagatgcta
     1741 aacgacgctt gcacaccaac ccgttacgca tattggatag taaaaatccg cgtatgcaag
     1801 ccatgtgtga agccgcacca aaattgattg attgtttggg ggatgaaacc cgtgcgcatt
     1861 tcgcgagtct atgtcagtta ttggatgacg caggtgttgc atatactatt aatgcgcgct
     1921 tggtacgtgg cttagattat tacaaccgca ctgtgtttga atgggtcacc accaagcttg
     1981 gcgcacaagg taccattgct ggtggcggtc gttatgatag cttggtggag cggttaggtg
     2041 gcaatccaac gcccgcctgt ggctttggca ttggtttaga gcgcgtattt ttgctgatgc
     2101 aagaatacgg cgtcattgct gaaaaggcgc ctgatattta tttggttaat gtgggtgaat
     2161 tggcggaaca acaagcattt aaagtggcgg aaactctacg tggtgcgggt ttaagtgttg
     2221 tacttcatgc agggggcggc agttttaaat cgcaaatgaa aaaagccgat agaagtggtg
     2281 cgcgttatgc agctattttg ggtgatgatg aagtgcaagc gaatcaaatt tcgctcaagc
     2341 ccttattgca atctggcgaa caggtaaaat gcgctgtgaa tcaggtggca agtagcattt
     2401 tagcgaagta aatttttgaa aaaataggat ggttaaatcc aactttacaa caacattaaa
     2461 tgttaatttt taaacaaacc attgcaccac ctctgcaagc cttattctac gaggcttcca
     2521 gaggcataaa aatcgctcgg tgcaaaagca tcttattttt aatgcatttt aaacactaat
     2581 tgacatgcca ttatttattc aaacaatgac acccttactg caaatttcaa atttaaccat
     2641 tacgcctaaa aagcagccca cacgtttgct ggtaaacggt gtgagtttta atattgaagc
     2701 aggtaaaacc acttgtgtcg tgggcgagtc aggtagcggt aaaagtttaa ccgccttagc
     2761 ggtgatgggc ttgttatcta agcaattgca attaaattcg ggcagtattt tgtttaataa
     2821 tgcgcaggca ggcgtacaag atttatctaa gcttgatatt gcgagtctgc aaaaaattcg
     2881 tggtgcaaaa atcgccatga tttttcaaga gccgatgacc tcgcttaacc ccgtattaac
     2941 ggtaggttat caaattggcg aatccttaac cgcgcattta ggcttaaaag gagcggcact
     3001 aaaagccaaa gttgccagct tgcttgaaat ggtgggtatt cctgcaggcc gtgcagatag
     3061 ttacccagat gaattatctg gcgggcaacg ccaacgcgtg atgattgcca tgagcattgc
     3121 ttgtgagcca caaatattga ttgcagacga gcccaccaca gcactagacg tcaccgtgca
     3181 ggcgcaaata cttcaacttt tgcatgagct taaaacacgc atgaacatgg gcatgctgtt
     3241 tattacgcat gattttggcg tggtggcaga tattgccgat aatgtggtgg tgatgtttag
     3301 aggtgaaatt gtagaatccg gtgctaaaga agccgtttta aacaacccac aacatcctta
     3361 tacacaggct ttattagctt gtgtccctga tgcagaaggt aaaaaaccat taaaacctat
     3421 tgattacagt tggttagggc atgcagcatg agtgaactaa ttcttgaagc aaatcatctc
     3481 gttaaaacct acacgcagcg tagcggtaga tttggtttta ataccacgtt agttaaagcg
     3541 ttagatgatg taagtgttgg tttgagtgct ggcaaaacgc ttgcggtagt tggggagtcg
     3601 gggagtggta aatccacgtt ggccagatgt ctgttacaat tgcacccttt tgatagcggc
     3661 gaagttattt ttcaaggtca gcagcttgat caattaaatg gacaagcatt gcgtcaaatg
     3721 cgacagtatt tgcaaatggt gttccaagac cctttcgcgt cattaaatcc acgtatgcgt
     3781 gtgggcgaga ttgtcggtga aggtttgtta attcatgggc ttggtagcag tagcgagcag
     3841 caaaaaaaag tagtggcaat gctaaagcgt gttggtttaa ctgaggcaga tatgcaaaaa
     3901 tatccgcatg aattttctgg cggacaacgg caacgggtgg gtattgcacg tgcattggtg
     3961 ttgcaaccta aagtggtcgt gtgtgatgag ccagtttctg cgctagatgt ttcaattcag
     4021 gcgcagatat tgttgttgct taaagaatta caagcagaaa tggcgctgag ttatctcttt
     4081 attagccatg atttacgtgt tgtgaggcat attgctgacg acgtagcggt gatgtttcaa
     4141 ggccgcattg ttgagcaggg cagtgtggca gatatttatg ataaccctag ggaagaatat
     4201 acaaggaagc tactatctgc aattccaggt aaggcaacgc aacattaaaa tatgtaaatg
     4261 ataggttttt gcgcgtttac taaatatata aaattttgaa tttgaataat cagtaaaaac
     4321 aaataggaaa aagtaatggc atacaattta gaagagcagg aacagataga cgaacttaaa
     4381 gcctggtgga agcaatatgg caaaacagtc agttcattag tgattggttt ggttgtggcg
     4441 tatgcggctt atcaaatttg gaattatgtg caacataagc aagcgattga ggcctcaact
     4501 cagtaccaag agttaacagt aactgatcaa aaagacttaa aagcaatcca agcaaaatct
     4561 gcaacattga tggagcaata tagcggaaca ccctatgctg cccgtgcagc gttgtttgcc
     4621 gcaaaagcta actatgcagc aaatgataca aaaagtgcta aagcgcagct agattgggca
     4681 attaaaaatg ctgaagaaac tgcaacgcgt gcaatggcca ctttgcagtt ggcaaatatc
     4741 ttagcagaag aaaaggacta tgaaggcgca ttgaaaatac tagatgcccc gcatgacgct
     4801 ggctttgatg gcttgtttgc tgatcttaaa ggggatgtgc tcatgcttca aggtaagcgc
     4861 gcggatgcga aagcggctta tgagcttgca ctttctaagt tggatgctca gggaaaatac
     4921 cgtgtgatta cacagcgtaa actagaagca ttaggctagt taaagtgaac gtaattgtca
     4981 cgactttata aattggagcg atgcgttgta cttaaaatca tatcaatcag tagcttataa
     5041 aacgttaggc attttagctt tattggttac aacctcttgc agcacactga cggatcttaa
     5101 aacggatatt tcggatagag tatttggacg ggaacctgcc gatgccccgg aagcgttggt
     5161 ggagtttaaa caaacagcga gcagtaaagt gttatggcaa gcaaaaatag gcccggctga
     5221 agattttgaa ttttcacctg tgattgaagc aggctttgcc tatgcagcag gtgccagcgg
     5281 agagctagtt aagttagata ttaacagcgg caaacaagaa tggcgcgtga acgcaggtga
     5341 aaaattatcc ggtggtgtag gcgtgggtgg cagcttggtg ttggtcggca catctaaagg
     5401 tgtggtctat gcttatgata atgcaggaaa attacagtgg aaatcaaaat taagtagcga
     5461 agtattaagt gcgcctagat attttgatgg cattgtgatt gtgcgtacca atgataatcg
     5521 catctacggc attaacgcag aggatggcag tcgcaagtgg gtgtatgaca gaacaagccc
     5581 agctttgaca ttgcgtagta gtgctggttt ggtcgtagat ggtggcgctg tgtatgctgg
     5641 ttttggtggc ggcaagctta ttgcactgcg tgctgataat ggcaaattgc tgtgggaagc
     5701 ttccgtggcc cagcccaaag gcgtgactga gattgagcgc attgccgata ttacgagctt
     5761 gcctgtgcta gatgggcctt tggtttacgc ggtggcttat caaggaagaa ttgctgcaat
     5821 cgaccgaatt tctggccgtg ttatttggaa tcgtgatata tcaagcttaa acggcattag
     5881 tgttgaagat ggccgtattt ttgtgcctca ctctatgggc tcggtctatg cgcttgacta
     5941 tacaacgggg aaaacgttct ggcgtcaggc cgctttgaaa aatcggcaac taacggcccc
     6001 tgtcccgatg gggaattatg tcgctgtggg tgatttagaa ggataccttc attttttaac
     6061 ccgtgatgaa ggcgcatttg cctcaagagt taaattagct ggtagtgcag taatgccaca
     6121 aatggtgttg cttaatagca ccactgtgct ggcacaaaca cgtgacggcg gcctttatgc
     6181 tgtgcaaata aagtagtaaa aatacattaa tagaatggct taacgaaagc gcggagcagg
     6241 ttatttaaaa ttcaacagca ttgatactgc tagttatttt gcaacctatt atcaagcgtt
     6301 atgttgtcat caatagaagg ttttcatgtt acccaccgtt gttctggttg gccgacccaa
     6361 tgtcggtaaa tcaactttat ttaatcgact gaccaaaagc cgcgatgcgc tcgttgcgga
     6421 tttgccgggg cttacgcgcg accgccatta tggccgcggt ttggggggca gtcagcctta
     6481 tttagtggtc gatacaggtg gatttgagcc taataccgat agcggcatcc tcaaagcgat
     6541 ggctacgcaa acattactgg ctattgatga ggctgatgtc attatattta ttgtggatgg
     6601 ccgtcaaggg gctacgccgc aagatatgga gatcgcgaat cggttacgcc gctgtaaatg
     6661 tcctgtgttg ctcgcggtta ataaaactga gggaatgaat aaagcggttg taagcgcaga
     6721 gtttcatgaa cttggcctgg gtgatccgtt gtcaatttca tcggcccacg gtgaaggcgt
     6781 gaaagatatt atagaactgg cacttgagca ttttcaagtg gttgaagatg cgcctgaaaa
     6841 tgaaaattca ggtgacagag tgcctaaggt tgccattgtt gggcgtccga atgtaggtaa
     6901 atcgactttg gtaaatgcgt tattgggtga ggagcgcgtg attgcgtttg accaaccagg
     6961 cacgacccga gattcaattt caattgattt agagaaaaat ggtaaacatt acacgctgat
     7021 agataccgct ggtgtacgca agcgtgggcg cgtactagaa gcgattgaaa agttttcagt
     7081 gattaaaacc attcagtcga ttgaagaagc caatgtggtg attttggtgg tggatgcgca
     7141 agaaggcatc accgaacaag atgcacacgt ggcagcttac atcctcgatg cgggccgtgc
     7201 attagtggtg gcgattaaca aatgggatgg tctcaaagaa gaagagcgcg attggattaa
     7261 acgagaaatt gaccgcaagc tgatgttttt agattttgcg gagtttcact atatttctgc
     7321 cttacgtaaa aaaggcctgc cagagttatt taaatcagtg gatgtggctt ttaaagcggc
     7381 atttgccaaa ctttcaaccc cgcaactgac gcgcgtgtta atagacgcgc ttcagcaaca
     7441 tcagccaccg attagcaagg gcatacgacc gaaattacgg tacgcgcatc aaggtggaag
     7501 caatccgccg attgtggtga ttcatggtag ccatgtggat ggtgttaaag acgcgtacac
     7561 acggtttttg gaaaaaactt ttcgcagaac ttttcagctt tcaggcacac ccttgcgtgt
     7621 gcagtacaaa caaggcgcca acccatttgc agaagatgaa gataagcgta aatctggcga
     7681 agggattgtg agtatgcgtc gccgaaaaac tgcgcaacgt gctgaattaa aagctaaaaa
     7741 aacggctgaa ttagaagcag gaaaagcaaa aaaacg
//
LOCUS       DAOQSI010000098         7671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_9166_length_7671_cov_4.776392, whole genome shotgun sequence.
ACCESSION   DAOQSI010000098 DAOQSI010000000
VERSION     DAOQSI010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 7671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7671
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9166_length_7671_cov_4.776392"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="PKL42_07075"
     CDS             complement(<1..977)
                     /locus_tag="PKL42_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNU66877.1"
                     /translation="MKSWFIYFKKNRDTALLTGALLCLIIALLKPTVPIKHNIYTYLL
                     IADISQSMNTVDMKVDGKPAARIAHTQQLMHETVSSLPCGTKVSIGLFAGNSVAALYN
                     PIEVCENFAAIQDTIDHLDWRTAWSGNSRIRESLYGISRIIRSFPEPAQVVLFTDGEE
                     APKLHAFNTKDLTSFQGADGWLLVGVGSEKGEAIPKLSDKNQVIGYWSNESFALQPGI
                     AQISESTLGTRDDNVAGANDRYISKLDAEYLTSVAKEIGATYIKGDSIHALLDAMKTQ
                     KPARRDVAPFNLSWVFAALAGLLLLGAYFTKHPIQQIASTLRLYKKEFKR"
     gene            complement(1005..1565)
                     /locus_tag="PKL42_07080"
     CDS             complement(1005..1565)
                     /locus_tag="PKL42_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66878.1"
                     /translation="MKLNTIRHWFSVKKITILLVLTALASAAGLAYELNRIRQIEAFN
                     LAVSTGKNPQTDKQSFEAKYATAYWLAKNERFKEATLLYSQLTQKGKPSQRAAVQHNI
                     GNMFFLKALQVSGGNDSKIRDEVEYLLMQAHSSYKQALKADNQHWGTRRNLDRVITLL
                     PENPTPGVGDSDTPGLILGNIPNGLP"
     gene            complement(1562..2551)
                     /locus_tag="PKL42_07085"
     CDS             complement(1562..2551)
                     /locus_tag="PKL42_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HNU66879.1"
                     /translation="MAFNQPLLLWLLPLALLPILLERSHTRTYSWLAMLPPDPLSNLI
                     GLLLKILASLALVCIILGLAGPHSLEQKVERIGVGAQIGMVIDRSASMDDPFSGGTVE
                     GRVGETKSVAAARIMSDFVGKRQNDMIGVITFSNSAMYVLPLTESREAILGAIKATAG
                     NSLFQTNIGGGLTSAVGLFEKVPDSGSRAIILISDGAGRVSSEVQQKLRDWLQRFNIS
                     LYWIVLRQPGGVSIFDTKYESNYEEALPTEVELYYYFKQLRTPFQAYEAENPKSLEVA
                     IADIDQKEKKPIKYMEKIPGRDFTQLCYLLATLMIALLLAVKYLEVKTWSTKT"
     gene            complement(2551..3570)
                     /locus_tag="PKL42_07090"
     CDS             complement(2551..3570)
                     /locus_tag="PKL42_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66880.1"
                     /translation="MKTSPLNIKNTSLLRVMQGLMLALTLHLSTAVADVKPLPDVDPK
                     FVTITETNPTRDAGYVVGDMLDRTVVIAIKKPYELVKESLPIVGYEHRWKGQLIGVEL
                     VDIKTKEKLKSDSATHTLDLRYQVFTTGRTVKHASLKAEILQVRNLQTKEVFQLRIPF
                     FDFRVSPLSIFGQVKLHEDLSPFVPPLLLDAGPEKRNLTLLAVLLGASLLGLLYIFGV
                     YAWLPKMGGPFAKAYRDIRKMPDNAEGLQNAVARVHQSLNRTAGNSLFASNIAEFVAS
                     KPAFAPVSSQIQQFFNLSKQVFFEPNAAVQLDDNPKAWLLKFCRQLRDCERGLRPENK
                     QKANA"
     gene            complement(3574..4437)
                     /locus_tag="PKL42_07095"
     CDS             complement(3574..4437)
                     /locus_tag="PKL42_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNU66881.1"
                     /translation="MSEEFIKEFTYHIAWRSRSRRPGRHKSNQRGMGMEFRGHTTLLS
                     YPDPRRIDIRQTIRDPMEQVHVRIFNQKSVTPVFVICDMSGSMQYGSTRKKIEIAADI
                     SQSVAQSVTHNRDLVGFIGFDDVLREDWLCTLSARPHTVIEMAENLRNYEAAEVGSHG
                     IVDTVRLLPRERSLIFMVSDFHMPISDLEESLVLMQRHHIVPIVLWDTSEYADLPEFG
                     ITSVTDPETGAKRTLFLRKEYRERILQGFIERRKIIEDLFLRYDMQPFFVENSFDADQ
                     LSEYFHQYVAA"
     gene            complement(4484..5500)
                     /locus_tag="PKL42_07100"
     CDS             complement(4484..5500)
                     /locus_tag="PKL42_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNU66882.1"
                     /translation="MSDTQLTDWRKHALTLESEVNKVVVGQQSPIRLITTSIFARGHV
                     LLEGDVGVGKTTLLRAFTRGIGGGYQRIEGTIDLMPNDLVYHTYLGEDGKPHVSAGPL
                     LQSGENLSIFFFNEINRARPQVHSLLLRVMAERTLTAFNKEHHFPHMLVFADRNQVEK
                     EETFDIPSAARDRFMMEIPIVVPNDDHIMEELVFNTRFHDVDTLVGNVPENILQFDQL
                     NAFGAMLQNSISASPALRSYVLKLWRATKNPTDYGVKVSGVDMKRLVLSGASPRGMGM
                     VLRAARVNAWLNERTAVLPEDIHDVFHSTVAHRLVFSPVYEMRRTEIAREMLSGIVNS
                     IAAP"
     gene            5854..>7671
                     /locus_tag="PKL42_07105"
     CDS             5854..>7671
                     /locus_tag="PKL42_07105"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HNU66883.1"
                     /translation="MTKTFASLFFSLTFSLLAQAAPDLDRAMKNPNNWAYPRGQYNNQ
                     GYSELSQINTRNVKNLKLAWTFSTGVNRGHEGAPLVIDGVMYLHTAFPNNVYALDLNN
                     DQKILWSYFPKQDPSTQAVLCCDNVNRGLGFGDGKIFLQQNNGVLVALDAKTGKVVWQ
                     VQVNDPKTGATNTNAPQVIKDKVLTGCAGGEFGVRCFMAAYYLKDGALAWKAYSTGPD
                     ADVLIGKDFNQANPQYSALSVYEDVNGGNKQGGSFKPLPNAQLKINEPELGVRTWLKP
                     QQEKDGWQNGGGAVTGYFSFDAKTNLVYYGTGNPGVWNPDVRPGDNKWASSVFARDVD
                     TGQAKWAMQTTPHDEWDYDATNEVILFEQKDKHNKTQTYATQINKNGFIYTWQADNGT
                     LIAAEKVHPFVNWAIGVDLKTGVPAKDPTYSVHQDVNTKGICPTVMGVKGVANPAAYS
                     TNTGLLYVPLNITCMTIESVSSKYQTAQPWVGATISQFSLGSKIQGLDAISNDGFGKI
                     AAINPLTKKTIWQNKEEFTLWSGVLSTSSNLVFYGTLHRWFKAVDAKTGKDLWRYQLG
                     SGSVSNPFTYSLKGRQFIGTYSALGGWASVAADLAHCGNG"
BASE COUNT         2188 a         1668 c         1697 g         2118 t
ORIGIN      
        1 cgtttgaatt cctttttata tagccgcagt gtgctcgcta tttgttgaat tgggtgcttt
       61 gtaaaataag cccccagtaa caataaccca gccaatgctg caaacaccca acttaaatta
      121 aagggtgcaa catcacggcg ggcaggtttt tgtgttttca ttgcatctag cagggcatgt
      181 atgctgtctc ccttaatgta agtcgcacca atttctttag caaccgaggt taaatattcg
      241 gcatctaact ttgaaatgta acgatcattt gcaccagcca cgttgtcatc gcgtgtccct
      301 aaggtagatt cagaaatttg cgcaatcccc ggctgcaagg caaaactctc gtttgaccag
      361 taaccaatga cttgattttt atcacttagt ttaggaatcg cttcaccttt ttctgaaccc
      421 acaccaacca ataaccaacc atctgcaccc tgaaagctgg ttaaatcttt ggtgttgaaa
      481 gcatgcagtt tcggggcttc ctctccgtca gtaaataaca caacctgcgc aggttcagga
      541 aagctgcgaa tgattctgga aatgccgtat aaactctcac gaatacgact gttgccagac
      601 catgctgtgc gccaatctaa gtggtcaatg gtgtcttgta tggcggcaaa attttcacaa
      661 acttcaatcg ggttataaag tgcggcaacg ctgttgcccg caaataaacc aatactcacc
      721 tttgtgccgc aaggcagcga agataccgtt tcatgcatca gttgttgcgt gtgtgcaata
      781 cgcgcggcag gttttccatc tactttcata tccactgtgt tcatgctttg tgaaatatca
      841 gcaatcagca aataggtgta aatgttatgc ttaatcggca ccgtgggctt aagcagcgcg
      901 atgattaaac atagcaatgc accagtgagc agtgccgtat cacgattttt tttaaaataa
      961 atgaaccagg atttcattta gttactttca aatcatttgt taaattaagg taaaccattg
     1021 gggatgttgc ccagtattaa acctggggta tcagagtctc ctacacctgg ggttggattt
     1081 tcaggtaata aagtaatcac gcggtcaaga tttcggcgtg ttccccaatg ttgattatct
     1141 gctttcagcg cctgtttata agagctgtgc gcctgcatga gtaggtattc aacttcatct
     1201 ctaattttag aatcattacc gccgctcact tgcagtgcct ttaagaaaaa catattgcca
     1261 atattgtgtt gcactgcagc tcgctggcta ggtttacctt tttgggtgag ttgtgaatac
     1321 aaaagcgtag cttctttaaa gcgctcattt tttgccaacc aataggcagt ggcatatttt
     1381 gcttcaaaac tttgcttatc agtttgcggg tttttaccag tactgaccgc cagattaaat
     1441 gcctcaattt ggcgaatacg gttgagttca taggcaagcc cagcagcgct tgcaagtgca
     1501 gtgagcacca gtaaaatagt gattttttta acactaaacc aatgtctaat tgtatttagt
     1561 ttcatgtttt tgtgctccag gtttttactt ctaaatattt gaccgctaaa agtagtgcaa
     1621 tcattaaggt agccagtagg tagcaaagtt gcgtgaaatc acggccagga attttttcca
     1681 tatatttaat cggttttttc tctttttggt cgatatctgc aatggcgact tcaagtgatt
     1741 tcgggttttc agcctcatac gcctgaaaag gcgtacgtag ctgcttaaag tagtagtaaa
     1801 gctctacttc tgtggggagt gcttcttcgt aatttgattc atacttggtg tcaaaaatac
     1861 tcacgccgcc cggttgtctc agcacaatcc aataaagtga aatattaaag cgttgtaacc
     1921 agtcacgcag tttttgctgc acttctgagc ttacgcggcc agcaccgtcc gaaattaaaa
     1981 taatggcgcg cgagcctgaa tcgggtactt tttcaaataa gccgacggca ctggtcaggc
     2041 caccgccaat attggtttga aaaagagagt tgcccgcagt ggctttgatg gcgcctaaaa
     2101 ttgcctcgcg actttcggta agcggtaaaa catacatggc agaattgctg aatgtaatca
     2161 cgcctatcat gtcattttgg cgtttgccga caaagtctga catgattcta gctgcagcaa
     2221 ctgatttagt ctcacccacg cgaccctcaa ccgtaccgcc agaaaagggg tcatccatac
     2281 tggcgcttct gtctataacc atgccgattt gcgcaccaac cccgatgcgc tcaacttttt
     2341 gctctaggct atgcgggcct gctaagccta aaattataca aacgagcgca agagacgcca
     2401 aaatttttaa caacaagccg atgaggtttg aaagcggatc aggtggcaac atggccaacc
     2461 acgaataagt gcgtgtgtgc gaacgctcaa gcaatattgg caataaagca agcggcaata
     2521 gccacaataa taatggttga ttaaatgcca ttacgcatta gccttttgct tgttttcagg
     2581 gcgcaggcca cgttcgcaat cgcgtaattg acggcaaaac ttcaataacc aagctttcgg
     2641 gttgtcatca agttggacag cggcattcgg ttcaaaaaag acttgtttcg atagattaaa
     2701 gaattgttga atttggctac taacgggagc aaaagcgggt ttcgatgcga caaactctgc
     2761 gatattgctt gcaaacaggc tattgccagc ggtgcgatta agcgattgat gaacacgtgc
     2821 taccgcattt tgcaagcctt cagcgttatc aggcatttta cgtatatcgc ggtaagcttt
     2881 tgcaaaaggg ccgcccattt ttggtagcca tgcatataca ccaaatatat agagcaaccc
     2941 aagcaatgat gcacctaaaa gcactgccag caaggttaaa ttacgttttt ctggaccagc
     3001 atctagcagc aatggcggca caaatgggct caaatcttca tggagtttta cttggccaaa
     3061 gatagataac ggtgaaacac gaaaatcaaa aaatggaata cgcaactgaa aaacttcttt
     3121 agtctgtaag tttcttactt gcagtatttc agcctttagt gaagcatgtt taacagttct
     3181 gccagtggta aatacttggt agcgtaagtc tagtgtgtgt gttgcgctgt ctgattttag
     3241 tttttctttt gtttttatgt caacaagctc aacaccaatt aattggcctt tccagcggtg
     3301 ttcatagcca acaatgggca gtgattcttt gacgagttca taaggttttt tgatggcaat
     3361 cacgaccgtt ctatcgagca tatctcccac cacgtaaccc gcatcgcgtg tgggattggt
     3421 ttctgtaatg gtgacaaact ttgggtctac atcaggcaac ggctttacat cagccacggc
     3481 tgtggaaaga tgcaaagtta acgctaacat gagcccttgc atcacacgca acaaagaagt
     3541 gtttttgatg tttaatggag atgttttcat agattaggct gccacatatt ggtgaaagta
     3601 ttcagataat tggtcggcat cgaaactatt ttctacaaaa aacggctgca tatcgtagcg
     3661 taaaaataaa tcttctatta tttttcgccg ctcaataaag ccttgtaaga tgcgttcgcg
     3721 atattcttta cgtaaaaata gggtgcgctt cgcgcctgtt tcagggtcgg ttacgctggt
     3781 aataccaaac tcaggtaaat ctgcgtattc tgaagtatcc caaagaacga taggtacaat
     3841 gtggtggcgt tgcattaaca cgagcgactc ttctaaatcg ctaataggca tatggaaatc
     3901 tgaaaccata aatatcaacg accgctctct gggcaagagt ctaaccgtat ccacaatgcc
     3961 gtggctacca acttcagcag cttcataatt tcttaggttt tctgccattt cgatgacggt
     4021 atgcggtctg gcagaaagtg tgcataacca gtcttcacgc aacacatcat caaagccgat
     4081 aaagccgact aaatcgcgat tatgggtgac ggactgcgcg actgactgac tgatatctgc
     4141 cgcaatttca atttttttac gcgtgctgcc atattgcata gagccggaca tatcgcaaat
     4201 tacaaacact ggcgtaacgc ttttttggtt aaaaatacgt acgtgcacct gctccatcgg
     4261 gtcgcgaatg gtttggcgaa tatcaatccg ccgcgggtca gggtatgaaa gcagggtggt
     4321 atggccacga aactccatgc ccatcccacg ctggttactt ttgtgcctac caggacggcg
     4381 cgaacgtgaa cgccacgcaa tatggtaggt aaattctttt ataaattctt cagacatcag
     4441 cgtcgcagta gttaagttgg tatttgtatg aggctgaata aacttaaggt gccgcaatgc
     4501 tattgacgat accacttaac atttcacgcg caatctctgt gcggcgcatt tcgtacaccg
     4561 ggctaaatac caagcggtgc gccacagtgg aatggaacac atcatgaata tcttctggca
     4621 aaacagcggt gcgttcattg agccaagcat tcacacgtgc tgcgcgcaat accatcccca
     4681 taccacgtgg gctggcgccc gataacacca agcgcttcat atcaacgcct gaaactttta
     4741 cgccgtaatc ggttgggttt ttagtcgcac gccaaagttt aagtacgtag ctacgcaatg
     4801 ctgggcttgc agaaatgctg ttttgcaaca tagcaccaaa ggcgttgagt tggtcaaact
     4861 gcaaaatgtt ttctggcaca ttacccacca gcgtatctac atcatgaaag cgcgtgttaa
     4921 acactaactc ttccataata tggtcatcat ttggcaccac aatagggatt tccatcataa
     4981 aacggtcacg cgcggcagat ggaatatcga acgtttcttc tttttctact tggttgcggt
     5041 cagcaaacac cagcatgtgc ggaaagtgat gctctttatt gaatgccgtt aaagtgcgct
     5101 cagccatcac gcgcaataac agcgagtgca cttgcggacg tgcgcggtta atctcgttaa
     5161 agaagaaaat agaaagattt tcaccagatt gcagtagcgg tcctgcgctc acgtgcggtt
     5221 tgccgtcttc acccaaataa gtgtgataca ccaagtcatt gggcattaag tcaatggtac
     5281 cttcaatacg ctgataaccg ccgccaatac cgcgtgtaaa tgcacgaagc agcgtggttt
     5341 tacctacgcc cacatcgcct tcaagcaaca catgtccacg cgcaaaaatt gaagtggtaa
     5401 ttaaacgaat cggtgattgt tgacccacga ccactttatt gacctcagac tctaaagtaa
     5461 gtgcgtgttt gcgccagtca gttagttgcg tatcgctcat ttgaagtgtt ccccaaagta
     5521 aattgtgatt gtgtgatttg ttaaaatttt gtaacagaat atgtcaattg taaattaatt
     5581 actatagccc gagtagggtc cagttttttt aattttgttc aacaggtttg taaaatttaa
     5641 tatttaacta aataagaata taaactaatt caacagaaaa atatacaggt ctgtatattt
     5701 tttaatgcca acggtctttg aaaaaatggt gctcaaatgg gattgctttt aataaactgt
     5761 aaattttaat catcacttat tttatttcat tgaaataact cacactaagt gagacacttt
     5821 gctcaaatta agcaaaccca aagtgagata acaatgacaa aaacattcgc atcactcttt
     5881 ttcagtttga ctttcagttt acttgcccaa gctgcgccag acctagaccg cgcaatgaaa
     5941 aaccccaaca actgggctta cccacgtggt caatacaaca accaaggcta tagcgaactg
     6001 agtcaaatca atacgcgcaa tgtcaaaaat ctcaaacttg cttggacatt tagcacaggc
     6061 gttaatcgtg gtcacgaagg cgcacctttg gtgattgatg gcgtaatgta tctacacacc
     6121 gcctttccta ataacgtcta tgcactggat ttaaacaatg accaaaagat tctatggagt
     6181 tactttccta aacaagaccc cagcacccaa gcggtgttgt gctgcgacaa cgtcaatcgc
     6241 gggttaggct ttggcgatgg caaaatattt ttacaacaga ataatggcgt actagtagcg
     6301 ctcgatgcca aaacaggcaa agtggtttgg caagtacaag tcaacgaccc taaaacaggc
     6361 gccaccaata ccaatgctcc gcaagtgatt aaagacaaag tactgactgg ctgtgcgggt
     6421 ggtgagtttg gtgtgcgatg ctttatggca gcctattacc tgaaagatgg cgcactcgct
     6481 tggaaggctt atagcacagg ccctgatgcc gatgtattga taggcaaaga ctttaatcag
     6541 gccaacccgc aatacagcgc cttaagtgtg tatgaagatg tgaacggcgg caacaaacaa
     6601 ggtggctcat ttaaaccttt gcccaatgcg caactcaaaa tcaacgagcc tgagctaggc
     6661 gtacgcactt ggctaaaacc gcaacaagag aaagatggct ggcagaatgg cggtggtgcg
     6721 gtcacaggct attttagctt tgacgcaaaa actaatttag tctattacgg cacaggcaac
     6781 ccgggggtgt ggaaccctga cgtacgcccc ggtgacaaca aatgggcgag cagtgtattt
     6841 gccagagatg tggacacagg ccaagccaaa tgggcaatgc aaaccacgcc gcatgatgag
     6901 tgggattatg atgcgactaa tgaggtgatt ttgtttgagc aaaaagacaa acacaacaaa
     6961 acccaaactt acgccacgca aattaacaag aatggcttta tttatacttg gcaggcagat
     7021 aacggcacct tgattgcggc agagaaggtg catccgtttg tgaattgggc gattggggtg
     7081 gatttgaaga caggggtgcc ggcgaaggat ccaacatata gcgtgcacca agatgttaat
     7141 acaaaaggga tttgtccaac agtgatgggc gtaaaaggtg tggcgaatcc tgctgcttat
     7201 tcaacaaata cgggtttact ctatgtgcca ctcaatatta cctgtatgac tatcgagtca
     7261 gtgtcatcaa aataccaaac tgcgcaacca tgggttggtg caactattag tcaattttca
     7321 cttggtagta aaatacaagg actagatgca attagtaatg atggttttgg caaaatagcc
     7381 gcaattaacc cattaactaa aaaaactatt tggcaaaata aagaggaatt tacactttgg
     7441 agcggagttt taagtacttc atccaattta gtattctatg gaacacttca tagatggttt
     7501 aaagccgtag atgctaaaac tggtaaagat ttatggcgtt atcagttagg ttctgggagt
     7561 gtgtcaaatc cgtttacata tagtttaaaa ggtcgtcagt ttattggcac gtactctgcg
     7621 ttaggtggtt gggcgagcgt tgctgcagac ctagctcatt gtggaaatgg t
//
LOCUS       DAOQSI010000099         7651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_9207_length_7651_cov_6.135334, whole genome shotgun sequence.
ACCESSION   DAOQSI010000099 DAOQSI010000000
VERSION     DAOQSI010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 7651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7651
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9207_length_7651_cov_6.135334"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..1305
                     /locus_tag="PKL42_07110"
     CDS             52..1305
                     /locus_tag="PKL42_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66884.1"
                     /translation="MILVLGGLAVVFSSLDGQSIRVERDKKTHDALAEAKLALIGYAT
                     RSGLATGAARPGELPCPDANNDGSSDVCAVNAVGRLPWRTLGINDLRDGDGERLWYAV
                     STSFKNNPRIYPLNSDTLGTISLRDSAGNMLNDGSANNGLVAVIVSPSAPMTRQDGVE
                     QVRDVANINNAINYMDIVLGEDNQNFVNGNVNGFISGPIKDATGNIILNDKLVSISRR
                     EIMMPVERRVAATVINALLDYFCGSGNVNYDTNACLAVGGSRSYPHPANFAESTCLGI
                     GNLAAGCTSDLAVNHGRLPANPNLSWDTTSLLLGSNGNWFQRNGWREQVHYAVAPECL
                     MGTTDCDGAGFLTLNTSLVNTVNVNVIVAVGGEMLAGQSRASVADKLNVTNYLEDENL
                     TTADDVYTNLSLMGNPFNDRVLSLP"
     gene            1305..2027
                     /locus_tag="PKL42_07115"
     CDS             1305..2027
                     /locus_tag="PKL42_07115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNU66885.1"
                     /translation="MRDKPNQLKGFTLIEMAVVLVILALLLGGLLLPLSAQLDQKNYT
                     DAKRQLESSMEAVMGYSLSNKHLPCPDKTAGANNGPNDNPNDGIEDFDVVAGTCITQE
                     GNLPWATLSLKDADSWNQRFIYRASAAFTVRAPIGTFGLTTNGVLRVCNEAACNVPRL
                     TDTAVAVIVSRGKNRGICTTLPSPPACADERENDDVDNDFVNHELRASSVASPNAEYD
                     DVVVWLSSNILVNRMVSAGQLP"
     gene            2027..2770
                     /gene="moeB"
                     /locus_tag="PKL42_07120"
     CDS             2027..2770
                     /gene="moeB"
                     /locus_tag="PKL42_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HNU66886.1"
                     /translation="MNDNQLLRYSRHILLPEIGYEGQDKLTKSHALIVGAGGLGSPVA
                     LYLAASGVGTLTICDFDVVDLTNLQRQIIHTTQSVGINKAVSAQQTLYEINPDVNVKT
                     IQKKSTEQELADLVAQADVVIDCSDNFATRYALNRQCVQHQKPLVSGAALKFEGQITV
                     YDMRASTSPCYHCLFPDNGEDTDLRCATNGVFSPLVGMVGTTQAAEALKILMGIGESL
                     QGRLLLLDALAMEWRTIRLKKDPACIVCG"
     gene            complement(2838..3680)
                     /locus_tag="PKL42_07125"
     CDS             complement(2838..3680)
                     /locus_tag="PKL42_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNU66887.1"
                     /translation="MFELSGILVIVAVILAFKGIRIVPQGEEWVVERLGKFAGVLSPG
                     LHVINPIFTKVTYKVTTKDIILDVPEQEVITRDNAVILANAVAFIKVSKIERAVYGIE
                     NFREAMRNMVQTSLRSIIGGMDLNQALTSRDRIKSELKTAIADEALDWGLTVKSVEIQ
                     DIKPSPNMQDAMERQAAAERERVAVVTEAEGAKQSLILNAEARLEAARKDAEAQMVAA
                     KASAESIKFITEAVKENNASAMFLLGDRYITALQKISDSENSKIVMMPGDLVGAVKSL
                     VGGK"
     gene            complement(3734..4177)
                     /locus_tag="PKL42_07130"
     CDS             complement(3734..4177)
                     /locus_tag="PKL42_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNU66888.1"
                     /translation="MTEAMWVWGVIGLVLLAAEMATGTFYVLWFGVAAIILAILTWLV
                     PDLNVSTQLILFSVLSIGALFIWRNYYKKTSVDHRVGQSQGEEIGRIGTIIVPISAAQ
                     TGRIQFTQGVMGSREWDAVSSEAIEIGRQAEVIAVEGNSLRVKAH"
     gene            4642..5871
                     /locus_tag="PKL42_07135"
     CDS             4642..5871
                     /locus_tag="PKL42_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HNU66889.1"
                     /translation="MKSAQKLIIPSDRGERWHYIKRMFSSAQGPDLDFNWAGGRLNGL
                     KRLHSIDVATYARNRNFLNGAVTHLSPYIRHGCITLPEAFNFVKKQNAPLADRLLMQL
                     AWREYWQQVWQIQGDAIFSELEPAKVMLGYQSMADDILKGKTGLPCMDGFIADLIKTG
                     YVHNHARMWLASYIVHHRKIDWRAGADWFEAHLLDGDFASNHLSWQWVASTFSSKPYF
                     TNKESLSRFTGEQYCAGCLAQCPFNASYEVLSSKLFDQTISPAAKQYVITHLPKKAVS
                     TSTAMSVFVHDEMLSAANPLLEKPFPKLFVFDPQIHGTWSLNRLQFVADSLSEMQDVE
                     VWLGDTYEVLLNRGVGRVITQDTPNRRIKELLEPFVPKYEPVVKLVKVEVSAQRLKRF
                     SRYWEKVGPHLLHTPAT"
     gene            6105..6665
                     /locus_tag="PKL42_07140"
     CDS             6105..6665
                     /locus_tag="PKL42_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNU66890.1"
                     /translation="MITLVVLAVVGIYFVITYNGLVNIKHNVEKAWANIDVLLKQRHE
                     ELPKLVDTCKQYMKFEQETLEKVIQARSRVSDARESHNVAALGVAENALRSGLGQLFA
                     LAESYPELKANEQFLHLQSRISGLENAIADRREFYNESVNINNVRIEQFPDVLVAKLF
                     NFKAADLLKFAAEELKDIDISQRFNG"
     gene            6674..7564
                     /locus_tag="PKL42_07145"
     CDS             6674..7564
                     /locus_tag="PKL42_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66891.1"
                     /translation="MLSGLRRYYIEAISSTSYLGILALSAYAYKDGSATFSILLMAVV
                     SVLAWISTTKRVRAIADIATSRIGSAAQGYVELYGRASAQADNLIKSPLSGMPCIWFR
                     YWVYSKENSKREWREVSRGVSDTTFEISDHTGKCQVDPDDAEVISPERRVSYQGVYKH
                     VEELLYAGGSVYVLGEFSTIGGANSVLSVKEDVGALLADWKKDPTQLKKRFDLDGNGE
                     IDTQEWELARKAAKREIEHLHREIRKESGVHIIRAPRDRRLFLISSMSPQKLRQRYQR
                     WSYFHFAVLVLSVGAFFWLM"
BASE COUNT         2098 a         1563 c         1853 g         2137 t
ORIGIN      
        1 taaaatcaac accaaaataa ccaacgaggg gcatcgttaa ttattttggt gttgatttta
       61 gttctgggtg ggttggcggt agttttttca agtcttgatg ggcagagtat tcgagttgag
      121 cgagataaaa agacccatga tgccttagca gaggcaaaat tagcgctgat tggctacgca
      181 accagaagtg gtctagctac aggcgccgct aggccaggtg agttgccttg cccagatgca
      241 aataatgatg gatcgtcaga cgtctgcgca gtgaatgcag taggaagatt gccttggaga
      301 acgcttggca tcaatgattt acgtgatggc gatggtgaaa gactttggta tgctgtttca
      361 acaagtttta agaataatcc aaggatatac ccacttaata gtgatacgtt agggactatt
      421 tcactgcgag attcagctgg aaatatgctt aatgacggat ctgcaaataa tgggttggtc
      481 gcagtgatag tttcgccaag tgcacctatg acacgtcagg atggcgtaga gcaggtgcga
      541 gatgtcgcaa acataaataa tgcgattaac tacatggaca ttgtgttggg cgaagacaat
      601 caaaactttg taaatggtaa cgtgaatgga tttatctccg gacctattaa agacgccact
      661 ggtaacatta tcctaaacga taaattagtt tcaatttctc gacgtgaaat tatgatgcca
      721 gttgaaagac gagttgcagc cacagttatc aacgcattat tagattattt ttgtggttca
      781 ggtaatgtga attacgacac gaacgcttgt cttgccgtag gtggctcaag aagctatccg
      841 catccggcaa atttcgctga gtcaacatgt ttaggaatag gaaacttagc cgctggttgc
      901 acttcagatt tggcggtaaa tcacggccga ttgcctgcta atccgaatct aagctgggac
      961 acaacttcat tgttgttggg ttcgaatggg aattggtttc aaagaaacgg ctggcgcgag
     1021 caggtacatt atgcagtggc accagaatgt cttatgggta caaccgattg tgatggcgca
     1081 ggttttctaa cattgaatac atcacttgtg aatacagtta atgtcaatgt gatagttgcg
     1141 gtgggtggcg agatgctcgc gggtcaatca cgtgcctcag ttgccgataa attaaatgta
     1201 acaaattatt tagaggatga aaatttgaca acagcagatg atgtttatac aaacttatct
     1261 ctaatgggaa atccattcaa cgaccgagtg ctgagccttc cataatgcgc gataaaccca
     1321 atcagttaaa aggatttact ttgatagaaa tggccgtcgt actggtcatt ttggcgttgc
     1381 tgttgggcgg tttgctttta cctttatctg cacagctaga tcaaaaaaat tacactgatg
     1441 caaaaaggca gttagagagt tcgatggaag ctgtgatggg gtatagcttg tctaataagc
     1501 acttaccctg cccagataaa actgctggag cgaacaatgg accaaatgat aaccccaatg
     1561 acggtataga agattttgat gtggtagccg ggacatgcat tacgcaagaa gggaatttgc
     1621 cgtgggcaac attaagtttg aaggatgctg attcgtggaa tcaacgattt atttatcgcg
     1681 cgagcgccgc ttttaccgta agggcgccaa tcggaacttt tggattaaca acaaatggtg
     1741 ttcttcgggt gtgtaatgaa gccgcatgta atgtaccgcg gttaacagat actgcggtag
     1801 ccgtaatcgt ttcgagagga aaaaatcggg gtatctgcac aacgttacct tcgccgcctg
     1861 cttgtgcaga tgaacgtgaa aatgacgatg ttgataacga ttttgtgaat catgaactaa
     1921 gagcaagtag tgtagcaagt cctaatgcag aatatgatga tgttgtcgtg tggctttcaa
     1981 gtaatatatt agtgaaccgt atggtgtcag caggtcagtt accctaatga acgacaatca
     2041 actcttacgt tacagccgcc atatcttgct gccagaaatt ggttatgaag ggcaagataa
     2101 gctcacaaaa agccatgcgc tgattgtggg tgcgggcggg ttgggttcgc cggtggcgct
     2161 gtatttggcg gcaagtggtg tgggtaccct caccatttgc gattttgatg tggtggattt
     2221 aaccaatttg cagcgtcaaa ttattcatac cacgcaaagt gtaggcatca ataaagccgt
     2281 ttcagcacag caaaccttgt atgaaattaa ccctgatgtg aacgttaaaa ccatacagaa
     2341 aaaatcaaca gaacaggaat tggccgactt ggtggcgcaa gcggacgttg tgattgattg
     2401 ctctgacaat tttgcaacgc gctatgcgct caacaggcag tgtgttcaac atcaaaaacc
     2461 gctggtttcg ggtgcggcgt taaagtttga ggggcagatt acggtgtacg atatgcgcgc
     2521 aagcacaagc ccttgttatc actgtttgtt ccccgataat ggtgaagaca ctgatttacg
     2581 ttgtgcgacg aatggcgttt tttcgccttt ggtgggaatg gttggtacta cgcaggcagc
     2641 tgaggcttta aaaatattga tgggaattgg cgagagtctg caagggcgct tattgctatt
     2701 agatgcgctt gcgatggagt ggcgcacaat taggctaaaa aaagacccag cttgtattgt
     2761 ttgtggctaa tgtttaatag taggggtgca atcaattgcg cccctacctt ctaaacgtgt
     2821 tccttatttc tattcgttta tttaccgccg actaagcttt tgacagcacc aaccaagtcg
     2881 cccggcatca tcacaatttt gctattctca gagtcggaaa ttttctgcaa cgcggtaatg
     2941 tagcggtcac ccaataaaaa catggcggaa gcgttatttt cttttacggc ttcggtaatg
     3001 aatttgattg attctgccga ggctttggct gcaaccattt gtgcttcagc atctttacgc
     3061 gctgcttcca ggcgtgcttc agcgttcaaa atgagtgatt gttttgcgcc ttcggcttct
     3121 gttaccacgg ccacacgttc acgctctgcc gcagcttggc gttccatcgc atcttgcata
     3181 tttggtgatg gtttaatgtc ttgtatttca accgatttaa ctgttaagcc ccagtcgagg
     3241 gcctcatcgg caatggcagt tttgagttcg cttttaatgc ggtcgcgtga ggtgagtgct
     3301 tggtttaaat ccatgccgcc gataatcgaa cgcaagctgg tttgcaccat gttacgcatg
     3361 gcttctctaa agttttcaat gccataaacc gcacgttcaa tttttgaaac tttaataaaa
     3421 gccacggcat tcgccaaaat caccgcattg tcacgcgtaa tgacttcttg ttcaggcaca
     3481 tctaaaataa tgtctttggt ggtgacttta taggtgactt ttgtaaaaat tgggttaatc
     3541 acatgcaagc cgggtgacaa aaccccggca aacttaccaa gacgctctac cacccactct
     3601 tcgccctgcg gcacaatgcg gatgccctta aaagctaaaa tcaccgccac aatgactaaa
     3661 ataccagata attcaaacat aattcactac ctttcagttg gtttatacaa attaaattct
     3721 ttaaaacagt gtgttagtgg gcttttacgc gcaaagagtt gccttctacg gcaattactt
     3781 ctgcctgtct gccaatttca atcgcctcgc tagaaacagc atcccactcg cggctgccca
     3841 tcacgccttg ggtaaattga atgcggcccg tttgtgcggc gctgattggc acaataatgg
     3901 tgccaatgcg gccaatttct tcaccttgtg attgccctac gcgatggtca acactggttt
     3961 ttttgtaata gttgcgccaa ataaagagcg cgccgattga gagcactgaa aataaaatca
     4021 gctgagtgct cacatttagg tcgggcacca gccaagttaa aatggcaaga ataatggcgg
     4081 caacgccaaa ccacaacaca taaaatgtac cggtcgccat ttcggctgcc agcaatacta
     4141 aaccgataac cccccaaacc cacattgctt cagtcatatt cttccttttt tattgacgaa
     4201 cgatgatggg ctattatggc atagaaaatt cttggcgtga aagtgcgcgc cgtgttttgc
     4261 aatgcgggtg gcaatggaag ttttgcaatt taaagccgta taaatgcccg aggatttttt
     4321 tatgctctgt gagcctttat ttataaggct cgcagagcat cgaaaaaggt tggtgcgttt
     4381 tttcataaaa aaatgatgaa cattaggtca aaaattgaat tcgaatggag tgcaaatttt
     4441 ttgtgaatgc atgtattttt tgcagtagtg tcacttaaaa cccagcaaag cacttgagca
     4501 atggagattt ctgggcacat ttatttgcag caaaagtaaa aaaagcatgt ttgaagcgtt
     4561 taaaacactc aagatgtcac aaaatatgac gtgaaaatag tacacttgca ttaaatgacg
     4621 gaataagtaa attactgtta aatgaaatct gcacaaaaac tgattattcc tagcgatcgg
     4681 ggtgaacgtt ggcattacat taagcgtatg ttttcatccg cccaagggcc agacttagac
     4741 tttaattggg cgggcgggcg cttgaatggc ctcaaaagat tgcatagcat tgatgtggca
     4801 acgtatgcgc gaaatcgcaa ttttttgaac ggggcggtca cgcatctttc accctatatt
     4861 cggcacggtt gtattacgtt gcccgaagca tttaattttg ttaaaaagca aaatgcgccg
     4921 ctggccgata gattgttaat gcaattggca tggcgagaat attggcagca agtatggcag
     4981 attcaagggg atgcgatttt tagcgaatta gagccagcca aagtgatgct tggctaccag
     5041 tccatggcag acgacatatt aaagggcaaa accggcttgc cctgtatgga tggctttatt
     5101 gcggacctca ttaaaacggg ttatgtacat aaccatgccc gtatgtggct ggcgagctat
     5161 attgtgcatc accgaaaaat cgactggcgc gcaggggccg attggtttga agcgcacttg
     5221 cttgatggcg actttgccag caatcacctt tcatggcagt gggtggcttc aacatttagt
     5281 tctaagcctt atttcaccaa taaagagagt ttaagccgct ttacgggtga acaatattgt
     5341 gcgggctgcc tagcgcagtg cccgtttaat gccagttatg aagtgctgag cagcaaattg
     5401 tttgaccaaa cgatttcgcc tgcggccaag caatacgtca ttacgcatct acccaaaaaa
     5461 gcggtttcaa cgtctaccgc catgtccgtg tttgtgcatg atgaaatgct ctccgccgcc
     5521 aaccccttgc ttgaaaaacc ttttccaaag ctatttgtgt ttgacccgca aattcacggc
     5581 acttggtcgc tcaaccgcct gcagtttgtg gctgatagcc tcagtgaaat gcaagatgta
     5641 gaagtttggt tgggcgacac ctacgaagtg ctgttaaacc gcggtgttgg gcgagtgatt
     5701 acgcaagata cgccaaatcg acgcataaaa gagttgcttg aaccctttgt gccgaaatac
     5761 gaaccggtgg ttaaattagt caaggttgaa gtgagtgcgc aacgcctaaa acgcttttcg
     5821 cgctattggg agaaagtggg cccgcatttg ctacatacgc ccgcaacata aggcgtttac
     5881 tagcgcaaaa caaccaaaaa tccgcatgat ttttcaacca acctttttcg atgccctgcg
     5941 agcctttatt tacaaggctc gcagggcatt aaatttttct cgtagatttt tgaatgattt
     6001 tccgcgctta ttttgtatcc acaaaatatc gttataatga cctttagttt aagtattttt
     6061 ttgaattttt ttgtaaaagc acgtcatttt atttaggaaa tagcatgatt actttagtgg
     6121 ttttagcagt tgtcggtatt tattttgtca tcacttataa cggcttggtc aacattaaac
     6181 acaatgttga aaaggcgtgg gcaaatattg atgtgctact caagcagcgt catgaagaat
     6241 tgccaaaact ggtagacact tgcaagcagt acatgaagtt tgagcaagaa acattagaaa
     6301 aagtcattca ggcgcgttca cgcgtgtcgg acgcacgcga gtcgcataat gtggctgcgc
     6361 tgggtgttgc agaaaatgca ttgagaagcg gcttgggcca attgtttgca ctggctgaat
     6421 cttacccgga acttaaagcc aacgagcagt ttttacattt gcagtcacgc attagtggcc
     6481 ttgaaaatgc cattgcggat aggcgcgagt tttataatga gagcgtgaat attaataatg
     6541 tgagaattga gcagtttcct gatgttttag tggcaaaatt atttaatttt aaagcggccg
     6601 atttgttgaa gtttgctgct gaagagttaa aagatattga tatcagtcaa cgctttaacg
     6661 ggtaggtgtc gctgtgttaa gtggcctgcg tagatattat attgaggcga tttcctcaac
     6721 aagctaccta ggcatactcg ccctatctgc ttatgcttat aaggacggct cagcgacctt
     6781 ttccatctta cttatggcag tggtgagtgt tttggcttgg atttctacca ctaaacgcgt
     6841 tcgcgcaatt gccgatattg ccacatcacg catcggctcg gccgcacaag gttatgtgga
     6901 gttatatggc cgcgccagtg cacaagcaga taatcttatt aaaagtccac tcagcggcat
     6961 gccttgcatc tggtttcgct actgggttta ttccaaagaa aacagtaagc gtgaatggcg
     7021 cgaagtgagc cgtggagtga gtgacactac ttttgaaatt agcgaccaca ctggcaaatg
     7081 ccaagttgat ccagacgatg cagaagtgat ttcacccgag cggcgcgtca gttatcaagg
     7141 tgtttataaa catgtggaag agttgctgta tgcgggtggc tcagtctatg tgctgggtga
     7201 attttcaacc ataggcgggg ctaattctgt acttagcgta aaagaagatg tgggggcatt
     7261 actcgcggac tggaaaaaag acccaacaca gctcaaaaaa cgcttcgatt tagacggcaa
     7321 tggtgagatt gatacgcaag agtgggaatt ggcgcgcaaa gccgccaaac gcgaaattga
     7381 acatttgcat cgcgagattc gcaaagaatc aggcgtgcat atcatccgcg ccccacgtga
     7441 ccgccggctg tttttaattt ccagtatgtc accacaaaag ctcaggcaac gttatcaaag
     7501 gtggagctat tttcactttg cagtgttggt tctgtcggtg ggtgcctttt tctggctgat
     7561 gtgaatcgtg aattttaagt ttacactgcg ctagacttcc acaaaagccg catgaggctg
     7621 tgttaaaatt gcacgcatga ctacgcaaaa t
//
LOCUS       DAOQSI010000100         7641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_9225_length_7641_cov_7.047060, whole genome shotgun sequence.
ACCESSION   DAOQSI010000100 DAOQSI010000000
VERSION     DAOQSI010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 7641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7641
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9225_length_7641_cov_7.047060"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(284..1210)
                     /locus_tag="PKL42_07150"
     CDS             complement(284..1210)
                     /locus_tag="PKL42_07150"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNU66892.1"
                     /translation="MAQNPIFSEQVRLLVSVLPSVAKQSCFALKGGTALNLFVRNLPR
                     LSVDIDLAYLPAGDREASLREIDRALESIATDIMSTVPNTQVNTSLVHGTDKRIKLLV
                     SRNNVSVKIEVTPVLRGCVYESELREVAPKVQAEFGYVQMQLLSFEDLYAGKLCAALD
                     RQHPRDLYDVRLLLANEGFSSKLKDAFIVYLLGHHRPMAELLMPRRKDIEAIYHAEFV
                     GMTLEEVSVESLLEAREAMIANIHRHLTEQDKQFLLAIKRGDADWSQFILPEVEHLPA
                     IQWKLHNLSMMKTDKRKQALAVLERALYFGSS"
     gene            complement(1185..2030)
                     /locus_tag="PKL42_07155"
     CDS             complement(1185..2030)
                     /locus_tag="PKL42_07155"
                     /inference="COORDINATES: protein motif:HMM:NF022898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HNU66893.1"
                     /translation="MSMQKPSSSKPPKNSIQEGSSESLENTLPEGLVVDRAWLNERGV
                     TRPSIDYYLRAGRLQAVARGAYRRPGPPLKWEHLVYSLQELGYPVHVGGRAALELQGY
                     THYVSFGGKQQIDLYGVRHIPPSLLTNNAPVKLELHSVELFSQLPAEAISTKPFGHWD
                     WSIRYATPELALLELLAAVNKDADFSVADKFFETAATLRPTLINALLSACTQVKAKRL
                     FLWFATRHNHSWLKSLDVSKVNLGSGKRVIVTGGLLDKQYQITVPREMRNSEEISDGS
                     ESNFF"
     gene            complement(2386..2461)
                     /locus_tag="PKL42_07160"
     tRNA            complement(2386..2461)
                     /locus_tag="PKL42_07160"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2426..2428),aa:Lys,seq:ttt)
     gene            complement(2600..3964)
                     /gene="ffh"
                     /locus_tag="PKL42_07165"
     CDS             complement(2600..3964)
                     /gene="ffh"
                     /locus_tag="PKL42_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNU66894.1"
                     /translation="MLDNLTGRLQGVIKTLRGHARLTEENITDAMREVRMALLEADVA
                     LPVVKEFIANVKARAQGKEVLASLTPGQAVIEVVNDELTTLMGKANVGLNLAAVPPAI
                     ILMAGLQGSGKTTTSGKLAKLLKEQKKKVLLASADVYRPAAIEQLKTLAKQLDVECFD
                     SNANQKPLDIAAATLDFAKRGYFDVVIFDTAGRLGIDEAMMAEIKALHTFLNPIETLF
                     VVDAMQGQDAVNTAKAFGETLPLTGVILTKLDGDSRGGAALSVRHVTGKPIKFIGVSE
                     KVDGLEPFYPDRMASRILGMGDVLGLIEQAHKNVDMAEAQKVADKIKSGKNFDLEDFK
                     MQIGQMRKMGGMQSLMDKLPAQLAQAAGQLQSNESDNAIRRMEGIINSMTALERRKPE
                     VIKATRKKRIADGAGVQVQEVNRLLKQFEETQKMMKMFSKGGMSKMMRAMGGMMGGKL
                     PGLR"
     gene            4111..4914
                     /gene="ccsA"
                     /locus_tag="PKL42_07170"
     CDS             4111..4914
                     /gene="ccsA"
                     /locus_tag="PKL42_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNU66895.1"
                     /translation="MQNLIPYLIVFFIYLAVAVDFWRIAKLAEKNLKLHSSMIALGLT
                     LHGWLLVHDVFAGAGINLGFFLALSAILWLTVLIYWLVNLKHPLYSLQAFVLPPAAVF
                     ALLPALTTQNHFLSTEGANLLLTHIGIALVAYSLFTFATLHALIMLVAERSLHQKKSW
                     IQLPDFPPLLVMEKLLFRVISLGFILLTITLVSGMVFSEEIFGKIAQFNHKTIFSIAA
                     WVIYGWLLFGHYQYGWRGKKAIKLTLIGFVLLLLAYVGTKFILEVIKQT"
     gene            complement(5022..5702)
                     /locus_tag="PKL42_07175"
     CDS             complement(5022..5702)
                     /locus_tag="PKL42_07175"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNU66896.1"
                     /translation="MNKNSTQNLALFDLDNTLLAGDSDFQWGQFMIAQGLLDKAAHHD
                     RNVQFHEDYKQGKLDIEAFLKFQLHPLSQHKRAFLDALHLEYMKQVIRPMMTDKARAL
                     VEKHRAQGDLLLVITATNSFITRPIALDFGIEHLIGTDPEEINGEFTGGFVGVPSFQT
                     GKVTRLNQWLAERGQTLADFEVSYFYSDSHNDLPLMKLVTNPVAVDADAELSAYATAQ
                     NWPQISLR"
     gene            complement(5699..6586)
                     /gene="yedA"
                     /locus_tag="PKL42_07180"
     CDS             complement(5699..6586)
                     /gene="yedA"
                     /locus_tag="PKL42_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HNU66897.1"
                     /translation="MTSKNQTLFIVAALFCTYFIWGSTYLAIRFGIESFPPFLMAGLR
                     FTVAGLILYVVMRFLGAPNPTKQQWLGASAVGILLPALGNGTVCYVQQTVSSSVAALA
                     IATAPIWMAIFSSIWGHRITRQEWLGIAIGLVGIVLLNLGGSLHGDVASALLLIFAAA
                     SWSFGSVWGKRLAMPQGLMAAAAQMLVGGVVLMIVSAYANEQWPAHISPKSWGAMLFL
                     VVLGSLVAYSAYQYLLKTVRPLVASSNTFVNPVVAFIVGIWLANEVVTTNEYIALAVI
                     LAGVILVLTATNEENHLKI"
     gene            complement(6786..>7641)
                     /locus_tag="PKL42_07185"
     CDS             complement(6786..>7641)
                     /locus_tag="PKL42_07185"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNU66898.1"
                     /translation="TINGLGERAGNASLEEVVMAIKTRRDIFNLDTRIDTTQIVPTSK
                     LVSSITGYPVQPNKAIVGANAFAHESGIHQDGVLKHRETYEIMRAEDVGWNANKLTLG
                     KLSGRNAFRTRLKELGIELESEDAVNAAFARFKDLADKKSEIFDEDLHALVSDEFVQE
                     ASENYKLVYLQVTSQTGEIPHAFITVSDNGAEQKAEADGGGPVDATFKAIENIVNSGV
                     ELQLYSVNNITSGTDAQGEVTVRLSKAGRIVNGQGADTDIVIASAKAYLNGLNKLHSK
                     LEKMNPQV"
BASE COUNT         2073 a         1859 c         1519 g         2190 t
ORIGIN      
        1 ttgaataatt tagggggcgt aattcggtat tttttatcct cttattagct aataactgaa
       61 gttgttttgc caaacctgta ggaagcttta gcccccccat tgttgccact caatcttgat
      121 ggatgagctc ccacatgggg tcgaaagtga aggcctatgt ccatcagtaa ttgggaattg
      181 aaattccaca atcaatcatg taaattgggc accaaaaaaa acagcaaatc aacatattta
      241 aagcacgact aaaatcttct ccaaacttca aatatacgca aatttacgaa gacccaaaat
      301 ataaagcacg ttcaagcacc gctagtgcct gttttcgttt atcggttttc atcattgata
      361 agttatgcaa tttccactga atagcgggta agtgctcaac ttctggaaga atgaattgac
      421 tccaatctgc atcaccgcgt ttaattgcta gtagaaactg tttatcctgc tccgttaaat
      481 gacgatgaat attagctatc atcgcttcac gtgcctcaag caggctttct acactgactt
      541 cttccaatgt cataccaaca aattctgcat gatatatagc ttcaatatct ttacggcgtg
      601 gcatcaacaa ttcagccatg ggtctatgat gccctaacag atacacaata aaggcatctt
      661 tcagttttga tgaaaatccc tcattggcaa gtagcaatct aacatcatat aaatcccgtg
      721 ggtgttgacg atccagtgcg gcacataact tgccggcata taaatcttca aagctaagca
      781 gttgcatctg cacatagcca aattccgctt gcacctttgg cgctacttca cgtaattcac
      841 tttcatatac gcaaccacgt aacacgggag tgacttcaat cttcacagag acattgttac
      901 gcgaaacgag caatttaatg cgtttgtcag ttccatgcac aagagacgta ttaacttgag
      961 tattgggtac agtactcata atatctgtgg cgatactttc taaagcgcga tcaatctctc
     1021 ttaaacttgc ttctctatcg ccagctggca aataagccaa atcaatatct accgataaac
     1081 gtggtaaatt acgtacaaat aaattaagcg cagtgccgcc ttttagcgca aagcaagatt
     1141 gttttgccac agagggcaaa acactcacta acaatctgac ttgctcagaa aaaattggat
     1201 tctgagccat cggatatttc ttctgagttt ctcatttctc ttggtacagt gatttgatac
     1261 tgtttgtcta atagaccacc tgtcactatc acacgcttgc cactacctaa attaacctta
     1321 gaaacatcca gactctttaa ccaggagtga ttatgccttg ttgcaaacca taaaaataag
     1381 cgttttgcct tcacttgcgt gcaagcactg agcaatgcat tgatcagtgt aggccgtagc
     1441 gtcgcagcag tctcaaaaaa tttatctgcc acagaaaagt ctgcgtcttt attcactgct
     1501 gctaacagtt ccagtaaagc caattcaggt gtagcgtagc gtattgacca atcccaatga
     1561 ccaaaaggtt tggttgaaat agcctcagca ggtaattggc taaataattc aacgctatga
     1621 agttccaatt ttactggcgc attgtttgtc aataatgatg gcggtatatg tcttactcca
     1681 tacaaatcta tctgttgctt cccaccaaaa gacacgtaat gtgtataacc ctgcaattct
     1741 aaagcggctc gtccaccaac atgtactgga tatcctagct cttgtaatga gtaaaccaag
     1801 tgttcccact taagcggtgg acctggacgg cgatatgcgc cacgcgcaac cgcttgcaag
     1861 cgccccgccc tcaaataata atctatacta ggtcgagtaa caccgcgctc attcagccac
     1921 gccctatcca ccaccaaccc ttcaggcagc gtgttttcca aagactcgct agacccttcc
     1981 tgtattgagt tcttaggggg tttagatgaa gatggttttt gcatactcat actatgcatt
     2041 attaatcatt attaaaccaa acgcaagttt attgatgctc tattttgcat agctaggcta
     2101 tgcaaaaatg gtaagttatg aaaataattt gggcgtacaa ttaagctttt ctgatagcaa
     2161 tcagcctgat tgtagaatgt aataaattca ttgaagaatg agctatgcag ttgttactcg
     2221 aactgggtaa gtgaaaatta cagggcatta cgactggtag cgtcggggac aaaaagtaaa
     2281 cccaagcaga tagtctaaat cacctaaaaa tgtctatttg cggaaaagta aggtatttgc
     2341 ggaaataaat agcgcggctt aacttccgtt aagccgcgct ggtattggta gggcgtgcgg
     2401 ggatcgaacc cacgacaaac ggattaaaag tccgctgctc taccagctga gctaacaccc
     2461 cattgagtag gttttatttc cctgcccacg aaagcccgcc attatgcaag aaagcgctgt
     2521 tatggtcaat gcgaatttga gaatattgtg caaatttact taactatttt tagttgcggc
     2581 tgtgcaaagc ccctaaaaac tagcgtaagc ccggtagttt tccacccatc atgccgccca
     2641 tggcacgcat cattttgctc atgccgcctt tgctgaacat tttcatcatt ttttgcgttt
     2701 cttcaaattg ctttagcagg cggtttactt cttgcacttg cacgcctgcg ccatcggcaa
     2761 tgcgtttttt acgcgtggcc ttgatgactt ctggcttgcg gcgctcaagg gctgtcatgc
     2821 tgttgataat gccttccata cggcgaattg cattatcgct ttcatttgat tgcaactgcc
     2881 ctgccgcctg cgccaattgt gctggcaact tatccatgag tgactgcata ccacccattt
     2941 tgcgcatttg gccaatttgc attttaaagt cttctaaatc aaagttttta ccagacttga
     3001 ttttatcggc gactttttgc gcctcggcca tatccacatt tttatgcgct tgctcaataa
     3061 gaccaagcac atcacccata cccaaaatgc ggcttgccat gcggtctggg taaaacggct
     3121 ctaatccatc aactttttca ctgacaccga taaatttaat cggtttaccc gtcacatggc
     3181 gcactgaaag ggccgcgccg ccgcgtgagt caccgtccag cttggttaaa attacacctg
     3241 ttaatggcag agtctcgcca aaggccttgg cagtattcac cgcgtcttgg ccttgcatgg
     3301 catcgaccac aaacaaagtt tcaatcgggt ttaaaaacgt atgtagcgct ttaatttccg
     3361 ccatcatcgc ttcatcaatg cccaagcggc ctgcggtatc gaaaatgact acgtcaaaat
     3421 aaccccgttt agcaaaatcg agtgtagccg cggcaatatc tagtggtttt tgattggcat
     3481 tgctatcaaa acattccaca tccaattgtt ttgccaatgt ttttagctgc tcaatcgcgg
     3541 caggccgata cacatccgca ctggccagca atactttttt cttttgttct ttgagcagtt
     3601 tggcgagttt gcctgaagtc gtagttttac cagaaccttg caagccagcc ataagaataa
     3661 tcgctggcgg cactgcggct agatttaagc ccacattggc tttacccatc agtgtagtca
     3721 gttcgtcatt caccacttca atgactgctt ggccaggcgt taagcttgca agcacttctt
     3781 tgccttgcgc gcgcgctttc acattggcaa taaactcttt caccacgggc agggcaacgt
     3841 cggcctcaag cagcgccata cgcacttcac gcatggcatc cgtaatattt tcttcagtga
     3901 gccttgcgtg gccgcgcaac gtttttatga cgccttgcag gcgaccggtc aaattatcta
     3961 gcatcatgtt cctctttaaa tgcgcttact tacaaattta tttaaaatta aattgcttct
     4021 acagcaaaat aatttcattt caggataatt aaaatctagg ataatcaaaa tcaatgtgca
     4081 attttaacct aaacacgcga taatttagct atgcaaaatc ttattcctta tttaattgtc
     4141 ttttttatct atttagccgt ggcagtggac ttttggcgta tcgccaaact ggctgaaaaa
     4201 aatctcaaat tgcacagttc gatgattgcg cttggtttaa cgttgcatgg ctggctgctt
     4261 gttcatgacg tgtttgcagg cgctggtatc aatttagggt ttttcttagc gctttccgcc
     4321 attctttggc tcactgtact catttattgg ttggtcaatt taaagcaccc gctttatagt
     4381 ctgcaagctt ttgttttacc acctgcggcg gtgtttgcgc tgcttcctgc gctcaccacg
     4441 caaaaccatt ttttatcgac cgaaggggca aatttattgc tcacgcatat tggtattgca
     4501 ctggtggctt atagtttatt tacgtttgcc acactgcacg cgctgattat gctggtggcc
     4561 gagcgtagtt tgcaccaaaa gaaaagctgg attcagttac cagattttcc accgttactg
     4621 gtgatggaaa aattattgtt ccgcgtcatc agccttggtt ttattttgct taccatcaca
     4681 ctggtaagtg gcatggtatt ttcagaagag atttttggca aaatcgcgca atttaaccac
     4741 aaaaccattt tttcaattgc cgcatgggta atttacggct ggcttttatt tgggcattat
     4801 caatatggct ggcgtggtaa aaaggccatt aaactgacgt taattggctt tgtgctactg
     4861 ctgctggcat acgttggaac gaagtttatt ttagaagtca ttaagcaaac ttaagcctat
     4921 ttttttagac acaacctttt ttaatgccct gtgagcctta taaacaaagg ctcgcagggc
     4981 attaaaattt tctcgggcga aaactcaccc attttcaatc attatcgcaa actgatttgt
     5041 ggccaatttt gcgctgtagc ataagcggat aattctgcgt cagcatccac tgccaccggg
     5101 ttggtcacta acttcatcaa gggcaagtcg ttatgtgaat cgctataaaa atagctcact
     5161 tcaaaatctg ctaacgtttg cccgcgctcg gctagccatt gatttaagcg cgtaacttta
     5221 cccgtttgaa agctcggcac gcccacaaac ccgccagtaa attcaccgtt gatttcttct
     5281 ggatcagtgc cgattaagtg ttcaatacca aaatccagcg caatcggacg cgtaataaaa
     5341 ctattggtgg cggtaatcac cagcaacaaa tcgccctgcg cacgatgctt ttcaaccagc
     5401 gcccgcgctt tgtccgtcat catcgggcgg atgacttgtt tcatgtattc caaatgcagc
     5461 gcgtctaaaa acgcgcgttt gtgctgcgat aatggatgca actgaaactt taaaaaagct
     5521 tcaatatcca gcttgccttg tttgtaatct tcatgaaact gcacgttgcg gtcgtgatgc
     5581 gcggctttat cgagcaggcc ttgtgcaatc ataaattgcc cccactgaaa atcgctatca
     5641 cccgcgagca aggtgttatc taagtcaaat aaagcaagat tttgtgttga atttttgttc
     5701 atatttttaa atggttttct tcatttgttg cggtgagaac taaaatcacg ccagctaaaa
     5761 tcaccgccag cgcaatatat tcattggtgg taacaacctc attggccagc caaatgccaa
     5821 caataaacgc caccaccggg ttaacaaagg tattgctgct ggccactaat ggacgcactg
     5881 ttttgagcaa atattggtaa gcactataag ccaccagcga acccaacacc actaaaaaca
     5941 acattgcgcc ccaagatttt gggctaatat gcgcaggcca ttgctcgtta gcgtaagcgc
     6001 tcactatcat cagaacaacc ccaccaacga gcatttgcgc agcggccgcc attaagcctt
     6061 gcggcatcgc caaacgtttg ccccaaactg agccgaacga ccaacttgcc gccgcaaaaa
     6121 ttagcaataa tgcgctggca acatcgccat gcaaactgcc gccaagattg agcaacacaa
     6181 tgcctaccaa gccaatggcg atacccagcc attcttgccg ggtgattctg tgcccccaaa
     6241 tacttgagaa aatcgccatc caaatcggcg cagtggctat cgccaatgcc gccacgctcg
     6301 atgataccgt ctgctgcaca tagcacactg tgccattgcc caaagccggc agtaaaatac
     6361 ccaccgcgct tgcacccaac cattgctgct ttgttgggtt tggcgcaccc aaaaaccgca
     6421 tgaccacata caaaatcaat cccgccacag taaagcgcag ccccgccatt aaaaatggtg
     6481 gaaaactttc aatgccaaac cgaatcgcta aatacgttga gccccaaata aagtaggtgc
     6541 aaaacaacgc ggcaacaatg aaaagagttt gatttttact ggtcatttaa aggcttaaca
     6601 aagccgaatt ttagattttg atagcgtcgt taaaacgcgt taaattagcc ttatttaaac
     6661 acaacctttt ttgatgctct ggaagccttt atttataagg cttccagagg cattattttt
     6721 tctctgcgca aaaagcgtgc gattcactca atttttatcg caaaaataag tgccaatgca
     6781 aggcgctaaa cttgcgggtt cattttctca agtttgctgt gcaatttatt caagccgttc
     6841 aaatacgctt tggcggaggc aatcacaata tcggtatcgg caccctggcc attcacaata
     6901 cggcctgctt ttgaaaggcg cacggtcact tccccttgcg catcggtgcc gctggtgatg
     6961 ttattgaccg aatacaattg caactcaacg ccactgttga cgatattttc aatcgcttta
     7021 aaagtagcat ccactgggcc accgccatcg gcttcggctt tttgttctgc gccattatct
     7081 gaaaccgtaa taaaagcatg tggaatttca cccgtttgcg aagtgacttg caaatacacc
     7141 aatttgtaat tttcagaagc ttcctgcaca aactcatcgc tcacaagcgc gtgcaaatct
     7201 tcatcaaaaa tttctgattt tttatcagcc aaatctttaa agcgcgcaaa cgcggcgtta
     7261 accgcatctt cgctttctaa ttctatgccc aattctttca agcgcgttct aaacgcattg
     7321 cggcctgaca atttgcccaa agtcaatttg ttggcattcc agcccacgtc ttctgcacgc
     7381 ataatttcgt aagtctcacg gtgctttaat acgccatctt gatgaatgcc gctttcgtgc
     7441 gcaaacgcat tggcgccgac aatcgcttta ttcggctgca ccgggtaacc cgtgatgcta
     7501 gaaaccaatt tgctggtcgg cacgatttgc gtggtatcaa tgcgcgtatc caaattaaaa
     7561 atatcgcggc gtgtttttat tgccatcacc acttcttcta agctggcatt acctgcacgt
     7621 tcgcccaagc cgttaatggt g
//
LOCUS       DAOQSI010000101         7639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_9227_length_7639_cov_6.252637, whole genome shotgun sequence.
ACCESSION   DAOQSI010000101 DAOQSI010000000
VERSION     DAOQSI010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 7639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7639
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9227_length_7639_cov_6.252637"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(705..1352)
                     /locus_tag="PKL42_07190"
     CDS             complement(705..1352)
                     /locus_tag="PKL42_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNU66899.1"
                     /translation="MKNNNKIRVMIADDHAILRAGLKQILMATDDIEVIAEAQNAHEA
                     MKLTTQLNPDVLLLDIAMPDRSGIEALKYIKKDNPHTAVLMLSMYKEEQYAMRALKCG
                     ASGYLCKQSASNELENAIRVVAKGKKYITPEVAEILATHVETETAKPAHELLSDREFQ
                     TLMMIASGQSVSEIAEKLSLSVKTVSMYRTRLLEKMQLRHNADITHYVIKNNLDA"
     gene            complement(1398..2453)
                     /locus_tag="PKL42_07195"
     CDS             complement(1398..2453)
                     /locus_tag="PKL42_07195"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNU66900.1"
                     /translation="MNAQELSLQIALSNFPGLVIQFHLDEKNQFVFDYLSDGCQALLG
                     RTPEELMQNPKQFLEMMNARERTLLYKRLQKSVESITSANWEGRVWIDGWQDTKWINL
                     RATPRVLMNGKVTWGGVMMNITQSKKEKIEVEASRRKLAELTAHLETIKEEERAKIAR
                     EIHDDLGGNLTVIKLGLSNIRNKLAENQAELKKNALGLETITDSTFDAIHRISRNLRP
                     TVLDLGIVDAIDWQVEEFKKQLGIDCQFDYNQSDIKTSSEQSMTLFRICQEALSNIAK
                     HAHATLVEVRLVRENCHVELSICDNGVGIKPKDKHKANSFGLHGMQERIEMQHGTISI
                     TKAKPRGTLIKVNLPLG"
     gene            complement(2738..4624)
                     /locus_tag="PKL42_07200"
     CDS             complement(2738..4624)
                     /locus_tag="PKL42_07200"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNU66901.1"
                     /translation="MSSAPKTANTKLSPVEIARETIKQMALKRVEPNPENYLVIYNEL
                     AGVTAKATLAEAVQKSLKTLPRDTPMQMNWVNRWEKMLKQQSWDELPTLMAAGMDAKV
                     NMAKQWPDAIRELVRGWDSKRADYDSNRKKETLERVLIKFGQDEQLAQKIQNLAQTWT
                     QVTPKTGTAIEVEVAEVNPATPSTTNIALVDNAVQSTAVTPKTAADEVAVTHDNTQLF
                     EMFNALKEMYKLSLKHGLIPRLEGYPDIELEAQKLLTEAERARKFKDWEAVAKHFRAL
                     LIRVEVIGANEVGIRQDILGLLKLLVDNISELVAEDQWLRGQVAVVQTILSSPLDRAL
                     IQSAEKSLKEIIYKQGTVKHSLVEAKAAFKHMLATFVDRLSYMSQTSGDYQEKISDYS
                     TQLSATDDIFKINEILEGIMSDTHVLQTDIVRSRELLIEQRDEAMTAQEKIRHLQDEL
                     TKLSEVVRVDQLTGVLNRRGFDDAVVREVSRVNRGGGNLSIAMLDVDNFKRLNDTHGH
                     HVGDEALQHLAKVIQDTVRPTDIVSRYGGEEFVILLPETNLDDALIAMTRLQRTLTKR
                     FFMGNNERLVITFSGGIALYKQDEDPVVSINRADQAMYLAKKTGKNRVLTEIDLEAKQ
                     MSIL"
     gene            complement(4908..6125)
                     /locus_tag="PKL42_07205"
     CDS             complement(4908..6125)
                     /locus_tag="PKL42_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HNU66902.1"
                     /translation="MRLFYSLNRTHLKTHSKLLVYVLASLAALAPFAIDTYLPAFHAM
                     ADDFATSDVHIQQSLTFYLLPYALMTLWHGAISDAIGRITTIKWGLGIFVLASIGCAF
                     APNVETLWFFRALQGASGGAGNTVARAMVRDLFEGPQAQRVMATVQMLFGIAPAVAPI
                     IGGLLLGIHWQAIFIFLAFYAALSLWAAAKFLPETMPPEKRLKLSAHNVWASYKTIFS
                     DHEYTKLCVAVGANFAAFFVYVLASPVFLGKLLHLTPQQYGYLFIPTVTGMVLGSYIA
                     KHVAGKYGAKSVLKAAFGWMLAMVLLNIVICYALPAKPLWNILPIALFNMGMALAMPI
                     LSLAALDRHPKIRGTAASGQAFVQMALSTISAGAIVPLVWGSVAGLAWVMAAYLLLGL
                     WVISTTKLFKQIA"
     gene            complement(6162..7394)
                     /gene="pqqE"
                     /locus_tag="PKL42_07210"
     CDS             complement(6162..7394)
                     /gene="pqqE"
                     /locus_tag="PKL42_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HNU66903.1"
                     /translation="MTQNSLGDSVVQKAVQSQQVNNGVLASATQTQPLWLLAEVTYRC
                     PLHCAFCYNPTDYDKHTQNELTTEEWIQTLRDARKLGAIQLGISGGEPLLRDDIEDIV
                     IEAKKLGYYSNLITSGVGLTEKRIQAFKEGGLDHIQLSMHDITEEINNFITNTKTFEL
                     KKKVAAMIKNHGYPMVLNVVIHRYNIGHMKEILEMAEALGADYIELANTQYYGWSLVN
                     RSQLMPTKEQVDEAERITNAHRANPDNKMKIFFVVPDYFADRPKKCMNGWGEVFMIVT
                     ANGDVLPCHSARVLPNMKFPNVKDSDIGWTWKDSPAFNQYRGDAWMKEPCRSCSEKAN
                     DLGGCRCQSFLLTGDAEAADPVCTKSPHRHLIDKAIEDAKNPQLQAQPIVFRNDKNSK
                     KIIEGEFQERLTEFHALP"
     gene            complement(7423..>7639)
                     /gene="pqqD"
                     /locus_tag="PKL42_07215"
     CDS             complement(7423..>7639)
                     /gene="pqqD"
                     /locus_tag="PKL42_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HNU66904.1"
                     /translation="EAQQCYVILFLEGMVKLNGGAGEVLKRVNGKASVADIVADIQST
                     FPDAPNIEADVVGMFDLAVGKAWLRKV"
BASE COUNT         2035 a         1777 c         1569 g         2258 t
ORIGIN      
        1 attactgatg ccatgataaa tctcctaaaa taaaataaag tttaatgtca ctttgactgc
       61 tttcgctttg caacttattc aattgcttgg cagttaaaac tattatcggt aagcgtttta
      121 aaaactttag gcttatcttt aactatttgt tttgatttgc cttttttaaa atcgcgaact
      181 ttattttttt aactcgtaat aaagcagccc gatgaatcta tttcggttgt gagtattaaa
      241 actttagaat tatttttgca ttttcctcgt ttcttgattc atttttaact tcaacacacg
      301 tttcattttg atgcgcgccc cggatatgca ttacctttgc aggcgatttg aatagcaata
      361 tcatagccaa tatttttttt taacaagggg acaatacaag gcaaaaaatg ccttaaatca
      421 aacgatgaga acagcagttt aagcagtgtt ttgtgtgaaa tttgtagcgg aaaagtgggc
      481 gctgaaatta aaaaaatcag gcaatttaaa agtgattttt aggaaaattt aatagcaacc
      541 cttttgacct gccctgcgag cctttattta taagtctcgc agggcattaa aaatctctcg
      601 ggagcaaaag cttaagaaaa atacgatttt taaaacaaat aaagagaata aaactggtaa
      661 aaaaagtaac gcaggctatt gcgccaagcg tttactttta atgtttaagc atccagatta
      721 tttttaataa cgtaatgcgt aatatcggcg ttgtggcgca gctgcatttt ttctaacaaa
      781 cgcgtgcggt acatactgac ggttttaact gaaagtgaaa gcttttcggc aatttcactc
      841 accgattggc ctgaggcaat catcattaag gtttgaaatt cacggtcaga gagtaactcg
      901 tgcgcgggtt tggctgtttc agtttcaaca tgcgtggcta aaatttcagc cacttctggc
      961 gtaatgtatt ttttaccttt cgccaccacg cgaatggcat tttctaactc gtttgaggcg
     1021 ctttgcttgc ataaatagcc tgaggcgccg catttgagtg cgcgcatggc gtattgctcc
     1081 tctttataca tagagagcat caacaccgcg gtgtgcgggt tatctttttt gatgtatttt
     1141 agcgcttcaa taccacttcg gtctggcatt gcaatatcca gtaacagcac atccggattt
     1201 aattgcgtgg tgagtttcat cgcttcatgc gcgttttgcg cttctgcaat cacttcaata
     1261 tcatcggtgg ccatgagtat ttgcttaagt cctgcgcgca aaatagcatg gtcatccgca
     1321 atcatcaccc tgattttatt attgtttttc atacttactt tctataactt catacaacag
     1381 cgcgatacta caccctctta gccaaggggc aaattgacct taatcaaggt tcccctaggt
     1441 tttgcttttg taatgctgat ggtgccgtgt tgcatttcaa tgcgttcttg catgccatgc
     1501 agaccaaacg agttggcttt atgtttatct ttaggcttga tgcccacgcc gttatcgcaa
     1561 atggatagtt caacatggca gttttcccgc accaaacgca cctcaaccag tgtggcatgg
     1621 gcgtgcttgg caatatttga gagcgcttct tggcaaatac gaaataatgt cattgattgc
     1681 tcagaggagg ttttgatgtc tgattggtta taatcaaact ggcagtcaat gcccaattgc
     1741 tttttgaatt cttcaacctg ccaatcaatc gcatccacaa tgcccaaatc aagtacggtg
     1801 gggcgcaaat ttcgcgaaat gcggtgaatg gcatcaaacg tgctgtcggt aatggtttcc
     1861 aaacctagcg catttttctt taactcggct tggttttctg ctaatttatt gcggatatta
     1921 gataacccta acttaatcac cgtcaggttt ccaccgaggt catcatgaat ttcgcgggca
     1981 atcttggcgc gttcttcttc tttaatggtt tctaagtggg ccgtcaattc agcgagttta
     2041 cgacgtgacg cctcaacttc aattttctct tttttacttt gcgtgatatt catcatcacg
     2101 ccaccccaag tgactttgcc gttcatcaat acgcgcggtg tggcgcgcag gttaatccat
     2161 ttggtatctt gccagccatc aatccacacg cggccttccc agttggcaga agtgatactt
     2221 tcaactgatt tttgtagccg tttatataat aaagtgcgct cgcgcgcatt catcatttct
     2281 aaaaactgtt tggggttttg catcaattct tctggcgttc tgcctaataa tgcttggcag
     2341 ccatcgctta agtaatcaaa cacaaattga tttttttcat ctaaatgaaa ttgaatgacc
     2401 aacccaggaa agttggacaa cgcgatttgt aaacttaatt cctgtgcgtt cattgatgag
     2461 aacaactaac ggcatggatt gacacggcag gagatgacac ggaataacac gaggacaatg
     2521 caaggattgt cagcattgcg caccaccttt ttgaaaaagt cgtcgccgca cgaaatgaaa
     2581 ttgcacgtta ttaaacaact tggccacccc taaaacttcg tttattgtta tgagtctatt
     2641 attagctttt tttaaacgaa atcaagctaa atgtaacaaa aatttacaat tagaggttgc
     2701 tctcatgatt ttcatgtgtc aagcacatga cattttccta caaaatgctc atttgcttgg
     2761 cttctaagtc aatttctgtc agtacacgat ttttaccggt ctttttagcc agatacatcg
     2821 cttggtctgc acggttgatt gacaccaccg gatcttcatc ttgtttatac agcgcaatgc
     2881 cgccactgaa ggtaatcaca agacgctcat tgttgcccat aaaaaaacgt ttggtgagcg
     2941 tgcgctgtaa gcgcgtcatg gcaatcaagg cgtcatccaa attggtttcg ggcaataaaa
     3001 tcacaaactc ttcaccaccg taacgactaa cgatatcggt tggccgaaca gtgtcttgta
     3061 tcactttggc aaggtgctgc agcgcttcat cgcccacgtg gtggccgtgt gtatcgttca
     3121 ggcgtttgaa attatccaca tccagcatgg caatgctcaa attgccgccg ccacgattca
     3181 cgcgtgaaac ttcacgcacc acggcatcat caaaaccgcg ccgattcagc acgcctgtaa
     3241 gttggtctac ccgcaccact tcacttaatt tcgttagttc gtcttgcaga tggcgaattt
     3301 tttcttgggc tgtcatcgct tcatcgcgct gctcaattaa caattcgcgc gagcgtacaa
     3361 tgtcggtttg cagtacgtgt gtgtctgaca taatgccttc aagaatttcg ttaatcttga
     3421 aaatatcatc agtggcagaa agttgcgtag aataatcgct gattttttct tggtaatcgc
     3481 cgctggtttg cgacatataa gacaggcggt ccacaaaagt cgccagcatg tgtttaaacg
     3541 ccgctttggc ttccaccaga ctgtgcttca ccgtgccttg tttataaatg atttctttca
     3601 ggcttttctc ggcactttga atgagcgcac ggtctagcgg gctagataat atcgtttgca
     3661 ccaccgccac ttgcccgcgc agccattggt cttcagccac taattctgaa atgttgtcca
     3721 ccagcaattt aagtaggccg agtatgtcct gacgtatacc cacctcattc gcgccgatta
     3781 cttcaacgcg gattagcaac gctctaaaat gcttagcaac ggcttcccag tctttgaatt
     3841 ttctcgcgcg ttcggcttca gtcagtaatt tttgcgcttc taattcaata tcagggtagc
     3901 cttctaaacg cggaatcaaa ccatgtttaa gcgatagttt gtacatctct tttaaggcat
     3961 taaacatttc aaaaagctgc gtattgtcat gcgtaacagc cacttcatct gcggctgttt
     4021 tgggggtgac agccgtgctt tgcactgcgt tatctaccaa ggcaatattg gtcgtgcttg
     4081 gtgtggcagg attaacttca gccacttcaa cctcaatggc ggtacccgtt tttggcgtaa
     4141 cttgtgtcca ggtttgggcg agattttgaa ttttttgcgc aagctgttca tcttggccaa
     4201 atttaattag cacgcgctct aaggtttctt ttttacgatt actgtcataa tctgcgcgtt
     4261 tactatccca gccgcgcacc agttctcgaa tcgcatcggg ccactgttta gccatattca
     4321 ctttggcatc catgccagca gccattaagg tgggcagttc atcccagctt tgctgcttga
     4381 gcattttttc ccagcgattc acccagttca tctgcatcgg cgtatcgcgt ggcagtgttt
     4441 taagcgattt ctgaacagct tctgccagtg ttgctttggc agtaacaccc gctaattcat
     4501 tgtaaatcac caaataattt tcaggattag gctcaacccg cttaagggcc atttgcttaa
     4561 tcgtttcgcg ggcaatttct accggagaaa gcttagtgtt tgcagttttt ggggcgctag
     4621 acataaataa tggtggcttc gcaataaaag ttagaaattt ctaccttttc attttgcgtg
     4681 agtgatagca aaaacaattt gaaatatagc gctacttttc aggcttaatt taactgtttc
     4741 aaccctaata agaatacgat gattttagtc agtattacgc cgagagaaaa ataatgctct
     4801 ggaagccttt atttataagg cttccagagc atcaaaaaag gttgcatgta aaatgcaagc
     4861 taaaaagact tcatttagtg catttttatg ccctgatttt tttaccctta ggcaatctgc
     4921 ttaaataact tggttgttga aatcacccat aatccgagta gcaaataagc agccatcacc
     4981 caggcaagcc cggctacgct cccccaaacc agcggcacaa tcgcgccagc cgatatggtg
     5041 gataatgcca tttgcacaaa cgcctgacct gaagccgccg tgccacgaat ttttggatgg
     5101 cgatcaaggg ctgccagaga aagaatgggc atggccagcg ccatgcccat gttaaataag
     5161 gcaatgggta aaatattcca cagcggtttc gcgggtaagg cataacagat cacaatgttt
     5221 aataacacca tggccaacat ccagccaaaa gccgctttta gcacactttt tgcgccatat
     5281 ttgcctgcaa catgctttgc aatatacgaa cccagcacca tgcctgtcac ggtgggaata
     5341 aacaaatagc catattgctg gggcgttaaa tgcagcaact tgcctaaaaa aaccgggctt
     5401 gccagcacat acacaaaaaa tgccgcaaaa tttgccccaa ccgctacaca aagtttggtg
     5461 tattcatggt cactaaagat ggttttatag ctcgcccaca cattatgcgc tgagagtttt
     5521 aaccgttttt caggcggcat ggtttcaggt aaaaattttg ccgccgccca caaactcaag
     5581 gccgcataaa acgccagaaa aataaaaatc gcttgccagt gtatgcctaa caaaagcccg
     5641 ccaatgatag gtgcaactgc gggcgcaatg ccaaacagca tttgcacggt tgccatgacg
     5701 cgttgcgcct gtgggccttc aaacagatca cgcaccatgg cgcgcgcaac agtattgccc
     5761 gcaccgccgc tagcgccctg caatgcacga aaaaaccaaa gcgtttcaac attgggcgca
     5821 aaagcacagc caatgctagc caatacaaaa atacccaacc cccacttaat ggtggtaatg
     5881 cgcccaatgg catctgaaat ggctccgtgc catagggtca tgagagcgta aggcaataaa
     5941 taaaaagtga ggctttgctg aatatgcacg tcagatgttg caaaatcatc cgccatggca
     6001 tgaaaagctg gcaaataagt atctatggca aaaggcgcga gcgcggctaa gcttgcgagt
     6061 acatagacaa gtaatttgga atgggttttc aaatgtgtac gattcagcga ataaaaaagg
     6121 cgcatcttta caaatgcgcc ttgaattata acggctattg attaaggcaa ggcatgaaac
     6181 tcggttaaac gttcttgaaa ttcaccttca ataatcttct tagagttttt atcatttcta
     6241 aacacaatcg gctgcgcctg caattgcggg tttttggcat cttcaatcgc cttgtcaatc
     6301 aaatgacggt gcggtgattt tgtgcacacc ggatcagccg cttcagcatc acctgtcagc
     6361 aagaaacttt ggcaacgaca gccgcctaaa tcgttggctt tttctgaaca actacggcat
     6421 ggttctttca tccaagcatc accgcgatat tgattaaacg cgggagaatc tttccatgtc
     6481 cagccaatat cgctgtcttt gacattggga aacttcatat tcggcagcac gcgcgcagaa
     6541 tggcatggca acacatcgcc attggcggtg acaatcataa atacttcgcc ccagccattc
     6601 atacattttt tagggcggtc agcaaaataa tctggcacca caaaaaagat tttcattttg
     6661 ttatcaggat tagctcggtg cgcattcgtg attctttctg cttcatccac ttgctctttg
     6721 gtgggcatca attggctacg attcaccaat gaccagccgt aatattgcgt attggctaac
     6781 tcaatgtaat ccgcccctag tgcctcagcc atttctaaaa tctctttcat atggccaatg
     6841 ttgtagcggt gaatcaccac attcaacacc atcggatagc cgtgattttt aatcatggcg
     6901 gccacttttt tcttcagctc aaaggtttta gtgttggtga taaaattgtt aatctcttcc
     6961 gtaatatcgt gcatggacaa ctgaatatgg tccaaaccac cttctttaaa cgcttgaatg
     7021 cgcttttctg tgaggccaac gcctgaagta atcaaattac tgtaataacc gagttttttt
     7081 gcctcaatca caatatcttc aatatcatcg cgcaatagcg gctcgccacc tgaaataccc
     7141 agttgaatcg cgcctaattt acgcgcatca cgcaaggttt gaatccattc ttctgtcgtt
     7201 agctcgtttt gtgtgtgctt gtcgtaatcg gttgggttat aacaaaaggc acaatgcagc
     7261 gggcaacgat aggtaacttc tgccagcaac cacaacggct gggtttgggt agcgcttgcc
     7321 aacacaccat tattgacctg ctggctttgc actgcttttt gtactactga atcacctagt
     7381 gaattttgtg tcatgtactg ctccttaatg gcaattacaa ctttagactt ttcttagcca
     7441 agctttgcca acggctaaat caaacatgcc caccacgtct gcctcaatat tgggcgcatc
     7501 tggaaatgta gattgtatat cagccacaat atcagccacg ctggctttgc cgtttacccg
     7561 cttgagcact tcacccgcac caccgttcaa tttcaccata ccttctagaa acaatatcac
     7621 gtagcattgt tgcgcttct
//
LOCUS       DAOQSI010000102         7600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_9303_length_7600_cov_4.139430, whole genome shotgun sequence.
ACCESSION   DAOQSI010000102 DAOQSI010000000
VERSION     DAOQSI010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9303_length_7600_cov_4.139430"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="PKL42_07220"
     CDS             <1..956
                     /locus_tag="PKL42_07220"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030602.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HNU66905.1"
                     /translation="AQAALPPAPCKIMMNVGNPDMAFGFAKTPNDGVGLARLEFVINN
                     MVGIHPKAILNAEAMPTAIKTIIQNRARGYASPKQFYIDKVAEGVATIAAAFYPKPVI
                     VRTSDFKSNEYKKLVGGEIYEPDEENPMIGFRGAARYMAEDFKECFAMECAAMKKVRD
                     EMGLTNVELMLPFVRTIDEAKAVTDIMAANGLKRGQNGLRLIMMCEIPSNALLAEEFL
                     HYFDGFSIGSNDLTQLTLGMDRDSGILADGFDERNDAVKVLLKMAISTCNRLGKYVGI
                     CGQGPSDHPDFAEWLVAEGIQSVSLNPDTVVATWQRLTKKS"
     gene            1008..1820
                     /locus_tag="PKL42_07225"
     CDS             1008..1820
                     /locus_tag="PKL42_07225"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HNU66906.1"
                     /translation="MQKRTVFFISDGTGITAGALGKLLEHFPSTNFTQVRLPFTDTLD
                     KIKLAQDAILHAAEEDANRPVVIMSLGDMELRNSIKKSDAYFIDLFNTFIDPLGVELK
                     QKPLTGAGIAHSVMGTKYNDRMEAVNFTLNHDDGMTNSGLEEAQVILIGVSRCGKTPT
                     SVYLAMQFGIKAANYPLIPEDFERGTLPAVLHKHLDKIFGLTIKPERLHAVRNERRAG
                     SFYASLDNCKKEIATAENLMRNAGITWADSTSRSVEELSAIILEKTRKPSNA"
     gene            complement(1934..2024)
                     /locus_tag="PKL42_07230"
     tRNA            complement(1934..2024)
                     /locus_tag="PKL42_07230"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1988..1990),aa:Ser,seq:gga)
     gene            complement(2216..3466)
                     /locus_tag="PKL42_07235"
     CDS             complement(2216..3466)
                     /locus_tag="PKL42_07235"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNU66907.1"
                     /translation="MSQALATSSIFTRDFWRKFSNPTSATLSKSILTFLVIAESLVCI
                     FWIFTLSGLGEGYALIAAVPYFYLVVSYFSLFLFYRFKRFEYFTFTQLVMLLVMPFFM
                     QWAIGGFAASSGVAIWAILCPVGALMILGTRQSTPWFLLFLGLAFVSWKLNPYFAGNA
                     MPIPTGVKDTFFLMNITGTASILYGVLRYFQAQKERVLHSLELEQARSEKLLLNILPA
                     PIAKRLKDNDMRIADHYDSVTVMFADLINFTQMSEKMTPNQLIELLSQVFVKFDELAE
                     KYQVEKIKTIGDSYMVISGAPIVCNDHAERIANMALEMHAVLKEVSQANGINLNMRIG
                     INTGPVVAGVIGSAKFSYDLWGDTVNMASRMESTGLLGEIQVSEETQRLLQKNYEFTA
                     RTGVEVKGKGILDTFILRHQKNNL"
     gene            3917..5437
                     /locus_tag="PKL42_07240"
     CDS             3917..5437
                     /locus_tag="PKL42_07240"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNU66908.1"
                     /translation="MSLELLKGLGVKIPYKAKYDNFIGGKWVAPVKGEYFDVITPITG
                     KPYTKAARSSAEDVELALDAAHAAADKWGKTAPAERANLLLKVADRLEQNLELIATAE
                     TIDNGKPIRETINADIPLAIDHFRYFAGCLRAQEGSLSELDETTVAYHFHEPLGVIGQ
                     IIPWNFPILMAAWKLAPAIAAGNCVVMKPAEFTPISILIMTEVIADILPPGVMNIVNG
                     YGREVGAPLANSKRIAKIAFTGSTATGRVIAQAAASNLIPATLELGGKSPNVFFEDIA
                     QYDDDFFDKAIEGLVLFAFNQGEVCTCPSRALIQESVYDKFMERVLPRVAAIKQGNPL
                     DPNTMIGAQASEDQMNKIMAYMDIGKQEGAELLIGGERNVLDGALAGGYYVKPTLLKG
                     HNKMRVFQEEIFGPVLAVTTFKDEADALAIANDTIFGLGSGVWSRNGNVAYRMGRGIK
                     AGRVWTNCYHAYPAHAAFGGYKESGIGRETHKMMLDHYQQTKNLLVSYSPKKLGFF"
     gene            5514..5894
                     /locus_tag="PKL42_07245"
     CDS             5514..5894
                     /locus_tag="PKL42_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HNU66909.1"
                     /translation="MAERVSATPSASALIQQLIAQHGPIVFFQSGGCCEGSVPLCLPA
                     NEFKPSASDIKLGEICKDATYFMSDSHFSFAENMHTIVDAVPGSSGSFSLDCGSGLAF
                     ITRARLYSDEELAQLPPVERCGFA"
     gene            6072..6168
                     /gene="ffs"
                     /locus_tag="PKL42_07250"
     ncRNA           6072..6168
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKL42_07250"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            6283..>7600
                     /gene="dnaX"
                     /locus_tag="PKL42_07255"
     CDS             6283..>7600
                     /gene="dnaX"
                     /locus_tag="PKL42_07255"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNU66910.1"
                     /translation="MSYQVLARKWRPKSFDTLVGQDHVVRALSNALTHNRLHHAYLFT
                     GTRGVGKTTLARILAKSLNCETGITATPCGVCNTCTEIDRGRYVDLIEVDAASNTQVD
                     AMRDLLDNAQYAPTSGRFKVYIIDEVHMLSKSAFNAMLKTLEEPPAHVKFILATTDPQ
                     KVPVTVLSRCLQFNLRQMAGTSIIGHLQHILTEENIPFEATALHLIARAANGSMRDAL
                     SLTDQAIAYGSQSVNEAEVRAMLGAIDQSYLYQILSALISNDGKRLIEQAKHMEERSI
                     SFEAALNDLAQLLHQISVAQTVPDSVADDLPERAQLLALAQQLPAETLQLYYQIAILG
                     RRDIGLAPDEFAGFTMSLMRMLAFTPVENSTQKTQSRPQTPPAIKATPETEKPSFSAP
                     ELAPQPIKAIQPTETFADTAINQDVMAFNGNWRGLIDKLKLGLARNL"
BASE COUNT         2206 a         1685 c         1642 g         2067 t
ORIGIN      
        1 aagcgcaagc tgcattgcca cctgcgccat gcaaaatcat gatgaacgta ggtaatcctg
       61 acatggcctt tggctttgcc aaaacgccaa atgacggcgt agggttggcg cgtttggaat
      121 tcgtaattaa taacatggtc ggcattcacc cgaaagcgat tttaaatgct gaggccatgc
      181 caaccgcgat taaaaccatt attcagaacc gtgcacgtgg ttacgctagc cctaaacagt
      241 tctacattga taaagtcgct gaaggcgtgg ccacgattgc agctgcattt tatccaaaac
      301 cggtgattgt gcgtacttca gactttaaat cgaacgagta caaaaaattg gttggtggtg
      361 aaatctacga gccagatgaa gaaaatccaa tgataggctt ccgcggtgcc gcacgttaca
      421 tggcagaaga cttcaaagag tgcttcgcaa tggaatgcgc cgccatgaaa aaagtgcgcg
      481 atgaaatggg cttaaccaat gtggaattga tgcttccatt cgtgcgtaca atagatgaag
      541 ccaaggcggt aaccgacatc atggcagcca acggacttaa acgtggtcaa aacggattac
      601 gcctcatcat gatgtgcgaa attccttcta atgcgctgtt agcggaagaa ttcttgcact
      661 attttgatgg cttctcaatc ggctcaaacg acctaaccca actcacctta ggcatggacc
      721 gtgactctgg tattttggcg gatggttttg atgaacgcaa cgatgccgtc aaagtattgc
      781 tgaaaatggc cattagcacc tgtaaccgcc taggcaaata tgtaggcatt tgtggtcaag
      841 gcccatcaga ccacccagat tttgcagagt ggttagtggc tgaaggcatc caatccgtgt
      901 cgctcaaccc tgacacagtg gtcgccactt ggcaaagact gactaaaaag tcataaatcg
      961 caacctgata aatgagtcat aagcgtattc gttaaggaaa agtcgcaatg caaaaacgta
     1021 ccgtattttt tatatctgat ggtactggca tcactgctgg cgccttgggt aaattgctgg
     1081 agcattttcc aagcaccaat tttacccaag tgcgacttcc ctttactgac acgcttgata
     1141 aaatcaaact agcgcaagac gctattttgc atgccgcaga agaagatgca aatcgccctg
     1201 ttgttatcat gtcacttggt gatatggaat taagaaattc gattaaaaaa tctgatgctt
     1261 attttatcga cctgtttaac acgtttattg acccacttgg ggttgagtta aagcaaaaac
     1321 ctctgactgg cgcgggtatt gcccacagcg tgatgggcac caaatataac gaccgtatgg
     1381 aagccgtcaa cttcacactc aaccacgatg acggcatgac gaattctggc ctagaagaag
     1441 cacaagtgat actcatcggc gtatcacgtt gcggtaaaac acctaccagt gtttatctcg
     1501 ccatgcagtt tggcattaaa gcggccaact atccgctaat accagaagac ttcgaacgcg
     1561 gcaccctacc cgctgtttta cataaacatc tagataaaat ttttggcctc accatcaaac
     1621 ccgagcgttt gcacgccgtg cgcaacgaac gtcgcgccgg cagtttttat gcctcattag
     1681 ataactgcaa aaaagaaatt gccacggctg aaaacttaat gcgtaacgca ggtatcacat
     1741 gggctgactc aaccagtcgt tctgtagaag aactatcagc aatcatattg gaaaaaaccc
     1801 gtaaaccttc gaacgcctaa ctagacaaaa tgaaatttta gagaagctaa tttcaccata
     1861 gaataaataa tgctattacc agttgcattt attgggttta ttaaacctga ataaaactag
     1921 ttgaatgaac cgctggcgga aggggcggga ttcgaacccg cgttaagtta ttcacctaaa
     1981 cacgctttcc aggcgtgcga cttaaaccgc tcatccaccc ttccgatatt ttgttgctgg
     2041 aaacttgctc agttaaaaac ttaattgcca aattaaaaat ttggcaatta atcaaaaact
     2101 ttaattactt ttgcttcaca ggttaaagat ttctagaacc ggacgcatta taaaacatat
     2161 cattacaata tgctgagggt aagtcattta attgatgcgt tgtcgcaaca aatttttata
     2221 gattattttt ttgatggcga agtatgaacg tatccaaaat gcctttaccc ttcacttcaa
     2281 cacctgttct tgcagtaaat tcgtaatttt tttgtaatag gcgctgcgtt tcttcagata
     2341 cttgaatttc tccaagtaag cctgtgcttt ccatacggct tgccatattg actgtatcac
     2401 cccataaatc atagctaaat tttgcactgc caatgacccc tgccaccact ggaccggtat
     2461 tgataccaat acgcatattt aaattaatac cgtttgcttg ggaaacttct tttaatacag
     2521 cgtgcatctc cagcgccata tttgcaatac gttcagcatg atcattacac acgataggtg
     2581 cgccggaaat caccatgtaa gagtcaccaa tggttttaat tttttctacc tgatattttt
     2641 ctgcaagttc atcaaatttt acaaacactt gtgacagcaa ttcaattaat tgatttggcg
     2701 tcattttttc agacatttgt gtaaaattaa tgaggtcagc aaacatcacc gtcactgaat
     2761 cataatggtc ggcaatgcgc atatcgttgt cttttaggcg ctttgcaatc ggtgctggca
     2821 atatattcag caagagcttt tctgagcgcg cttgttctag ttctagtgaa tgcaatacac
     2881 gctctttttg tgcctgaaaa taacgtaaca cgccatatag aatggatgcc gtgccagtga
     2941 tattcattaa gaaaaaggta tcttttacgc cagttggtat tggcatagca ttgccagcaa
     3001 agtaaggatt gagcttccaa gacacaaagg ctaagcctaa aaacagcaaa aaccaagggg
     3061 ttgattgacg tgtgcccaat atcatcagcg cacccactgg gcaaagtata gcccagattg
     3121 ccacgccact ggaggctgca aatccgccaa tcgcccactg cataaaaaac ggcattacaa
     3181 gcaacatgac gagttgcgtg aaagtgaaat attcaaaacg cttaaatcga taaaataaga
     3241 aaagactgaa gtatgaaaca actaggtaaa agtaaggaac tgctgcaatt aacgcatagc
     3301 cttcaccaag gcctgagagg gtgaatatcc aaaagataca gactaaactt tcagcaatca
     3361 ccaaaaaagt taaaatggat ttacttagtg ttgcagacgt ggggttggaa aactttctcc
     3421 aaaagtcacg cgtaaatatc gacgatgtag ctaaagcttg tgacatatga cctatcgtaa
     3481 aaatgaaccg atttgtaaat caattgttac atatttttca aaagaatgtt gtaaattata
     3541 tgatttttca acttagtgat gtttaaaagc ttttgtttta tgagctaaaa tgaacaaggt
     3601 tttggtcgat actttgagta gcatgtgaaa ataaacagtt gttgattttt tacttcagac
     3661 tgccacttta aacagtcact ttgaaaaatg tcgtacattc gcataaatat gttttatgtc
     3721 ttatatgcat tataggcttg atttgcgcag ctattaggtt tattgttgtg ttcaaactgt
     3781 ggtgtgcaag agtccagtat ttataagcct tacagggcga ttgtgctagt tttaaataat
     3841 agcaaaaagt ttctttttag tgcggtttag caggtagatt tactatatta acgatagaaa
     3901 aactagagga gttagcatga gtttagagtt actaaaaggc cttggtgtaa aaatccctta
     3961 caaagccaaa tacgataatt ttattggtgg caaatgggtt gcccctgtta agggtgaata
     4021 ttttgatgtt atcaccccaa ttacgggtaa gccttacaca aaagcggcac gttccagtgc
     4081 tgaagatgtt gaacttgcgt tagatgctgc ccacgctgct gctgataaat ggggtaaaac
     4141 agcacctgca gaacgcgcaa atttactcct taaagtggct gatcgccttg aacaaaatct
     4201 agaattaatc gcaacggccg aaactattga taacggtaaa ccgattcgtg aaactatcaa
     4261 cgcagatatt ccgttagcca ttgaccattt ccgttatttt gccggctgtt tacgcgcgca
     4321 agaaggctcg ctcagtgagt tagacgaaac cactgtggct tatcattttc atgagcctct
     4381 cggcgttatt ggccaaatta tcccttggaa cttcccgatt ttaatggcgg cctggaaact
     4441 cgcccccgca attgcagcag gtaattgcgt ggtaatgaaa cccgctgaat ttacccctat
     4501 cagcatttta atcatgacag aagtcattgc tgacatttta ccgccaggcg tgatgaacat
     4561 tgtgaatggt tacggccgtg aagtcggcgc accgttagcg aacagcaaac gtattgcaaa
     4621 aatcgccttt actggctcaa cagccactgg ccgcgtgatt gcgcaggcag cagcatcaaa
     4681 tctcattcct gcaacactag aattaggcgg caaatcgcct aacgtgttct ttgaagacat
     4741 tgcccaatat gacgatgact tttttgataa agccattgaa ggtttggtgc tatttgcctt
     4801 taaccaaggt gaagtttgta cttgcccatc acgtgccttg attcaagagt ctgtctacga
     4861 caaatttatg gaacgcgtgt taccacgtgt tgcggcgatt aaacaaggca acccacttga
     4921 cccaaacacc atgattggtg cgcaggcttc tgaagatcag atgaataaaa tcatggcata
     4981 catggatatt ggtaagcaag aaggtgcaga attactcatt ggtggtgagc gcaatgtgtt
     5041 agatggcgca ttggcaggcg gctattatgt taagcctacc ctattaaaag gccataacaa
     5101 aatgcgcgtt ttccaagaag aaatttttgg cccagtactc gctgtgacga cctttaaaga
     5161 tgaagcggac gcacttgcca ttgccaatga cactattttt ggtctgggct caggcgtttg
     5221 gagccgtaat ggcaacgttg cctatcgcat gggtcgtggc atcaaggctg gtcgtgtttg
     5281 gacaaactgc taccatgcat acccagcaca tgccgcattt ggtggttaca aagagtctgg
     5341 cattggtcgt gaaacacaca aaatgatgct tgaccattac caacaaacca aaaacctatt
     5401 ggtaagctac agccctaaaa agcttggctt cttctaatgt tttagagttt cgctaagaga
     5461 ttagtgatac tagaggcgag tttactcgcc tcttcctttt tggagatgac atcatggccg
     5521 aacgtgtttc tgcaacaccc agtgctagcg cattgattca acagttaatt gcccaacatg
     5581 ggccgattgt attttttcaa tccggcggct gctgtgaagg cagcgtaccg ctttgcttgc
     5641 ccgccaacga atttaaaccc agtgcttcag atatcaagtt aggcgaaatc tgcaaagatg
     5701 ctacctattt tatgagcgat agccattttt cgtttgctga aaacatgcac acgattgtgg
     5761 atgcggtgcc gggttccagt ggctcatttt cgctagattg tggctcaggt ctagccttta
     5821 ttacccgcgc gcgtttgtat agtgatgaag agttagccca attaccgccc gttgagcgtt
     5881 gtggatttgc ctagctaatt tttgcaaata ttaaggcgtt tacacaccaa ccctttacac
     5941 ctgccctgca agcctttatt tataaggctc acagggcagc gtttttctct cgtagtaaaa
     6001 agtaagtaaa tttttatatt taaccgtcaa aatagatagt caattagggc aaataaagta
     6061 taattgcgct cggcgggtct cctcgcattg taaagtagcc aatctggtca ggtgcggaag
     6121 caagcagcca caactattga gcaagtgccg aggttaaggc tcgccacttc tttttttcaa
     6181 atcaagcaac accaaatcag ccgcattttt atgcataaaa gtagagacgt tgccgcgctt
     6241 ttaggctaaa gtttcactca tgttaaataa gcatttttac ctatgagcta ccaagttctg
     6301 gcacgtaaat ggcgaccaaa atcgtttgat accttggtgg gccaagacca tgtagtgcgt
     6361 gcgctcagca atgcactcac gcataaccgt ctgcatcatg cctatttgtt caccggcacg
     6421 cgcggcgtag gtaaaaccac cctcgcccgc attttagcta aatcgctcaa ttgtgaaacc
     6481 ggcatcacgg caacgccatg cggcgtatgc aatacctgta ctgaaattga cagaggccgc
     6541 tatgtagacc tcattgaagt ggatgctgcc agtaacacgc aagtggatgc tatgcgcgat
     6601 ttactggata acgcgcaata cgcacccact tctggccgct ttaaagtgta tatcatcgac
     6661 gaagtgcata tgttgtctaa aagtgcattt aacgcgatgt taaagacgct ggaagagcca
     6721 cctgcgcacg tcaaattcat tcttgccacc actgatccgc aaaaagtacc agtgacggtg
     6781 ctttcgcgct gtttgcagtt taatttacgg caaatggctg gcacgtcgat tatcggccac
     6841 ctgcaacata ttcttacaga agaaaatata ccgtttgaag ccaccgcgct gcatttaatt
     6901 gcccgtgcgg ctaatggcag tatgcgtgat gccttatcgc taactgacca agccattgct
     6961 tatggcagcc aatccgtaaa tgaagcagaa gttcgcgcca tgctgggtgc aattgaccag
     7021 agttatttat accaaatttt aagtgcgctt atctcaaatg atggcaaacg ccttatcgaa
     7081 caagccaagc acatggaaga acgcagcatt tcgtttgaag cggcgcttaa tgatttggcg
     7141 cagcttctac atcaaatcag cgtggcgcaa accgtgcctg atagcgtggc cgatgacttg
     7201 ccagagcgcg cgcagttgtt agccttagcg caacaattgc cggctgaaac tctgcagtta
     7261 tattatcaga ttgccatttt aggtcggcgc gatatcggtt tggcgccaga tgagtttgct
     7321 gggtttacca tgagcctgat gcgcatgctg gcgtttactc ctgttgaaaa cagcacgcaa
     7381 aaaacacaaa gcagaccgca aacgccacct gcaataaaag caacacccga gactgaaaaa
     7441 ccttcttttt cagcaccaga attagccccg caaccaatta aagccatcca gccaacagaa
     7501 acttttgctg atactgccat caatcaagat gtgatggctt ttaatggcaa ctggcgtggt
     7561 ctgattgata agctcaaatt aggcttagca cgcaatctag
//
LOCUS       DAOQSI010000103         7510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_9483_length_7510_cov_5.596647, whole genome shotgun sequence.
ACCESSION   DAOQSI010000103 DAOQSI010000000
VERSION     DAOQSI010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 7510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7510
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9483_length_7510_cov_5.596647"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..1321
                     /gene="ppk2"
                     /locus_tag="PKL42_07260"
     CDS             173..1321
                     /gene="ppk2"
                     /locus_tag="PKL42_07260"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HNU66911.1"
                     /translation="MASTKSTSKTNSNATVVNHPSNPATLTPDNVANITVNKEIAAAH
                     ESKAAAVADIVEHMAGLDPAQITDALQAILNGASPDDAAILRSMLIGTAAGVEKSNRN
                     ADTALASDWREGGYPYKNLLSRRSYEKQKYKLQVELLKLQAWTKETGQRVVIIFEGRD
                     AAGKGGAIKRFMEHLNPRGARVVALEKPSEVERGQWYFQRYVSELPTAGEIVLFDRSW
                     YNRAGVERVMGFCSNDEYLEFMREAPEFEKHLVRSGIHLIKFWFSVSRKEQRRRFKER
                     EVHPLKQWKLSPIDLASLEKWDDYTKAKEAMFFHTDTAESPWTVVKSDCKKRARLNAM
                     RYVLHKLPYKNKSLDAIGAVDPLLVGRAQVVYERGEKELTVLDQGALL"
     gene            1666..2109
                     /locus_tag="PKL42_07265"
     CDS             1666..2109
                     /locus_tag="PKL42_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNU66912.1"
                     /translation="MKINAKQHIIDADLRPTSARIAVLSVISCADSALSHSEILERLV
                     TQKEFDRVTIYRVLDWLTHHHLVHKISGENRAWKFQISQHTHHADAHHHAHLLCTVCG
                     KVSCIPELSPQFPKPILKKYHVETIDVHFKGLCEDCFSASQPPQN"
     gene            2194..2937
                     /locus_tag="PKL42_07270"
     CDS             2194..2937
                     /locus_tag="PKL42_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNU66913.1"
                     /translation="MTLLQIILATLVGGVFSVLLASLVALTFLARFANRMVAFAVGVL
                     LGFALTDILPEAVNLGLDITQAGWILIAGIMGFFLLEKLALWRHDHATHLGKTQHKPQ
                     VTMIVIGDGMHNFVDGVLLAATFLTDWKLGWAAAIAIIAHEIPQEISDFMVLLDAGVS
                     KRKALILNALSGAAMTLGGVIGWLSLSNAHAAIPYILVLAAASFIYIAVADLVPELHH
                     HRKPRDMALQLSLMAAGLGVVALNKLLLN"
     gene            2948..3934
                     /locus_tag="PKL42_07275"
     CDS             2948..3934
                     /locus_tag="PKL42_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNU66914.1"
                     /translation="MTTTSTDKRIPATILTGFLGAGKTTLLNRILSEQHGQRIAVIEN
                     EFGEAGVDGDLLIQGEEQIVEMNNGCICCTVRGDLVRILGDLSQRNQAGELRFDRVII
                     ETTGLADPAPVAQTFFVEEAIREQYKLDAIITVVDAVHGMQQLDEHHEALEQVGFADR
                     ILLSKTDLVTDEAVQAITARLRQINPRAPIKNVHFGKADIADILDIGGFVLDEILKIE
                     PDFLEDVSHEHDDEIGSFVFRSERAFDLQKLEVFISGLLEIYGTQLLRYKGILNIAGD
                     DHKTVFQGVHMLMGGEQGSTWKPNEKRESVLVFIGKALPRNAFEKGLAMCLV"
     gene            3972..5189
                     /gene="zigA"
                     /locus_tag="PKL42_07280"
     CDS             3972..5189
                     /gene="zigA"
                     /locus_tag="PKL42_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallochaperone GTPase ZigA"
                     /protein_id="HNU66915.1"
                     /translation="MKKLPVTVLSGFLGAGKTTLLNHILNNREGKRVAVIVNDMSEVN
                     IDAQLVRDGGAALSRQDEKMVEMSNGCICCTLREDLLIEITRLAKENRFDYLLIETTG
                     IAEPLPIAETFTFADEDGVSLSDVAQLDTMVTVVDAYNFLKDYSSRDFIADRGESLGD
                     DDERTVVDLLIEQIEFCDVLVLNKTDLLTKDEIARLEALLKTFNPRADIAHASFGKVP
                     LARVMNTGKFNFDDAANAPGWLQELRGEHVPETEEYGISSFVYCARQPFHPQRLWHWF
                     NQEWAGVIRSKGHFWIASRPEYCLNWSQAGAITRTEPAGFWWAASPKNYWPEDKEQLQ
                     MIQDRWQEPYGDRQQEIVIIGMQMNRDAIIAGFDACLLNAQEIALGMDGWKLLDDPFP
                     KWNAIHDEADEVN"
     gene            5210..6226
                     /locus_tag="PKL42_07285"
     CDS             5210..6226
                     /locus_tag="PKL42_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNU66916.1"
                     /translation="MDTRIPVTLLTGFLGSGKTTLLNKLLANPRMKDSAVIINELGST
                     RLDHILATQVESEHIADNTVLLESGCICCSLKNELADTMRDLFFKRALQAIPQFNRLV
                     IETTGMADPGPILGNLMNEPVIESVFRLDAVVVTVDSAYGLKQLQEHLEARKQAAVAD
                     VLLLTKTDLASADEVNALKEKLIEINPGATQHKIVHGDIDPDFIIDVGLFDPTGKRAE
                     PERWLRAPLKVARPKGTLPQASQHDEISSFTVTLPSPLNWRDLKPVILKLCQTHGDKL
                     LRLKGIIHAADQAAPLAIHAVHFTPYPPTLLEGWTEDEPENRIVLIGKGLDEYAIRKL
                     LTQV"
     gene            complement(6384..7307)
                     /locus_tag="PKL42_07290"
     CDS             complement(6384..7307)
                     /locus_tag="PKL42_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HNU66917.1"
                     /translation="MRTAYISHPEFLKHDMGPDHPECPARMYAITDQLIASGILDYLN
                     HYEASKVGKEILALAHSVDYIDWVYQNAPSDGLIDLDGDTLMNPYTLDAALYATGAVV
                     NAVDLVLSGEVNNAFCNIRPPGHHAKRNGASGFCIFNNVAVAAAYALEHRGLQRVAIA
                     DFDVHHCDGTEDIFRDDPRVMLCSTFQHPYYPYCGAESGNEHIINVPLAAGTSGEGFR
                     SAVEERWLPALTGFEPELILISAGFDAHREDDMGGLALHEKDYLWVTQQLKKIAEQFC
                     HKRIVSALEGGYELHALGRSVMTHVKCLAEL"
     gene            complement(7315..>7510)
                     /locus_tag="PKL42_07295"
     CDS             complement(7315..>7510)
                     /locus_tag="PKL42_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNU66918.1"
                     /translation="MRGTGLGHKLMTTLINIARSKGLKRIEGEVLKNNSSMLKLMKRL
                     SFSAKTNEDDTNIKDVYLDL"
BASE COUNT         2112 a         1725 c         1734 g         1939 t
ORIGIN      
        1 aataaaggct cgcagggcat tatttttttc tcgggcgatg ttaatgccga ttaaatcaca
       61 ttgacgcacc ttaattttca gctatcttta acctgtttga catccgtggt tgacaagccc
      121 atctattggc ataccattat gcttaactag gtaaaaatga cgaggtcaaa ctatggcaag
      181 cacaaaaagt acaagcaaaa caaattcaaa cgcaacggtg gtgaatcacc caagcaaccc
      241 tgctacgctc accccagaca atgtggcgaa tatcaccgtc aataaagaaa ttgccgcagc
      301 gcatgaatca aaagccgccg ctgttgcaga cattgttgag cacatggcag gtttagaccc
      361 cgcgcaaatt accgatgccc tgcaagccat attaaatggc gcttcccccg atgacgcggc
      421 aattcttaga agcatgttaa ttggcaccgc ggccggtgta gaaaaatcta acagaaatgc
      481 cgataccgcg cttgccagcg attggcgcga aggcggctac ccttataaaa acctgctctc
      541 gcgccgcagt tatgaaaaac aaaaatacaa actgcaagta gagttactca aactacaagc
      601 gtggaccaaa gaaactggcc agcgcgtagt gattattttt gaaggccgcg acgccgccgg
      661 caaaggtggc gccattaaac gctttatgga acaccttaac ccacgcggcg cacgcgtagt
      721 ggcgcttgaa aaacccagtg aagttgagcg cggccaatgg tattttcaac gctatgtgtc
      781 agaactaccc accgccggcg aaattgtgct gtttgaccgc tcttggtaca accgcgctgg
      841 cgttgagcgc gtaatgggtt tttgcagcaa tgacgaatat ttagaattta tgcgtgaagc
      901 gccagaattt gaaaaacact tggtgcgtag cggcattcac ctcattaaat tctggttttc
      961 tgtgagccgc aaagagcaac gccgtcgctt taaagagcgt gaagtacatc cactaaaaca
     1021 atggaaactc agcccgattg acttagcctc acttgaaaaa tgggacgact acaccaaagc
     1081 caaagaagcc atgttttttc ataccgacac cgccgaaagc ccttggacgg tagtgaaatc
     1141 ggactgcaaa aaacgcgcgc gtttaaacgc catgcgctac gtgctgcaca aattgcccta
     1201 taaaaacaaa agcctcgatg cgattggcgc ggttgatcca ctgttagttg ggcgcgcgca
     1261 agtagtgtat gaacgcggtg aaaaagaact gacggtgtta gaccaaggcg ctttgcttta
     1321 acacaacttt ttagcgcatt tttaaagcta aaaacccagc ctaaaagctg ggtttttgtt
     1381 tgcttaaaaa acactaaaaa accattcaaa acacatcaac cctttttagc acctctgtaa
     1441 gcctttattt ataaggctcg cagaggcata aaaaatcctc gggcgattct gttcttgtaa
     1501 aaactatttt tgtgtattta caacactaaa ttttcaaagc cctagtgtaa aaacgttaca
     1561 atacacaaaa aaacatcatg acccgttact actctagggc tacttaaaac aacaagttag
     1621 ccatagctaa tgaagggtat tacaaaacat gaataggcta tgcccatgaa aatcaacgct
     1681 aaacaacata tcattgatgc agatttacgt cctaccagtg cccgcattgc tgtgcttagc
     1741 gtcattagtt gcgcagacag cgcattgagt cactctgaaa ttttagaacg cctagtcaca
     1801 caaaaggagt ttgaccgtgt gaccatttac cgtgtgctag attggcttac ccatcaccat
     1861 ttagtgcata aaatttcagg cgaaaaccgc gcttggaagt ttcaaatttc tcaacacacg
     1921 catcacgctg acgcacacca ccatgcacat ttactatgca ccgtttgcgg aaaagtttct
     1981 tgcatcccag aattgtcccc gcaatttcct aagcccatat taaaaaaata tcacgttgaa
     2041 accatcgatg ttcattttaa aggcttatgt gaagattgtt ttagcgcttc acaaccaccc
     2101 caaaattaaa taatttttta acaaatgcaa ttttgttgca tttgtttgcc caccccacta
     2161 aaatgcaaca ttgttgcttt ttagtctttc taaatgacac ttctacaaat tatcttagcc
     2221 accttggtgg gtggcgtatt ttcagtcttg cttgcaagct tggttgcgct cactttttta
     2281 gcccgctttg ccaaccgcat ggtggcgttt gcggtgggcg tgctgctagg ttttgcgctc
     2341 accgatattt tgcctgaagc ggttaatcta ggtttagata ttacccaagc aggctggata
     2401 ttgattgcag gcatcatggg tttttttctg ttagaaaaac ttgccctttg gcggcacgac
     2461 catgccacgc acttaggcaa aactcagcat aaacctcaag tcaccatgat agtgattggc
     2521 gatggtatgc ataactttgt ggatggtgta ttgctggcgg caacgttttt aaccgattgg
     2581 aagttaggct gggcggcggc cattgccatt attgcccatg aaattccgca agaaatctcc
     2641 gactttatgg tgctgttaga tgcgggcgtt tcaaaacgca aggccttaat tcttaatgcg
     2701 ctttcaggcg cggccatgac cttaggcggt gtgattggct ggttgagttt aagcaatgcc
     2761 catgcggcca ttccctacat attggtgctg gcggccgcta gttttattta tattgccgtg
     2821 gccgatttag tgcctgaatt acatcaccat cgcaagccgc gtgatatggc cttgcaactg
     2881 agcttaatgg cggctgggct aggcgtggtg gcgttgaaca aactattact caactgaagg
     2941 acttaacatg acgacaacaa gcaccgataa acgcatccct gccacaatcc tcactgggtt
     3001 tttgggcgca ggcaaaacca cgctgcttaa ccgcatttta agcgagcagc atgggcagcg
     3061 cattgccgta attgaaaatg aatttggtga agcaggcgtt gacggcgact tgctgattca
     3121 aggggaagag caaattgtag aaatgaataa cggctgcatt tgctgcactg tgcgtggcga
     3181 tttagtgcgg attttaggtg atttatcaca acgtaatcaa gctggcgaat tgaggtttga
     3241 ccgagtgatt attgaaacca ctggcctagc tgacccagcc cctgtggcac aaacgttttt
     3301 tgtagaagag gcgatacgcg aacaatacaa gctagatgcc attattaccg tagttgatgc
     3361 cgtgcatggc atgcaacaac ttgacgaaca ccatgaagcg ctggagcaag taggctttgc
     3421 tgatcggatt ttgctctcaa aaacggattt agtgacagat gaagcagtac aagctatcac
     3481 cgcaaggctg cgccaaatta acccgcgcgc gccgattaaa aacgtgcatt ttggcaaagc
     3541 agacattgcc gatattttag atattggcgg ttttgtactc gatgaaatcc tcaaaattga
     3601 gcctgacttt ttagaagacg tgagccacga gcatgatgat gaaattggct cgtttgtgtt
     3661 tcgcagcgaa cgtgcttttg acctgcaaaa actagaggtg tttatcagcg ggttgctcga
     3721 aatttacggc actcagttgc tgcgctacaa aggcattttg aatattgcgg gggacgacca
     3781 taaaaccgta tttcaaggcg tacatatgct catgggcggc gaacaaggct cgacttggaa
     3841 acccaatgaa aagcgtgaat cggtattggt gtttattggt aaagcgttgc cacgaaatgc
     3901 ttttgaaaaa ggtttagcaa tgtgcttggt ttaaaacccg ctttttgcat taattataat
     3961 ttggaaaaca aatgaaaaaa ctcccagtga ctgtgttgtc aggtttttta ggcgcgggta
     4021 aaaccactct gcttaatcac attttaaaca atcgcgaagg caagcgcgtg gcggtgattg
     4081 taaacgatat gtctgaagta aacattgacg cgcaactggt gcgcgatggt ggtgctgcgc
     4141 tctcacgcca agatgaaaaa atggtggaaa tgagcaacgg ttgcatttgc tgcacgctac
     4201 gagaagattt attgatagaa attacacgct tagctaaaga aaaccggttt gactatttgc
     4261 tgattgaaac cactggcatt gccgaaccat tgcccattgc cgaaaccttc acttttgccg
     4321 atgaagatgg cgtgagctta tcggatgtag cgcaattaga caccatggtt accgtagtgg
     4381 atgcttacaa ctttttaaaa gattacagct cccgtgactt tattgccgac cgtggtgaat
     4441 cgctggggga tgatgatgag cgcacagtag tagatttact gattgagcaa attgaatttt
     4501 gcgatgtgtt agtactcaac aaaactgatt tattaacaaa agatgaaatt gcacggcttg
     4561 aagcactgct taaaaccttt aacccacgcg ccgacattgc gcatgcaagc tttggcaaag
     4621 taccgcttgc acgtgtaatg aacactggta agtttaactt tgacgatgcc gccaacgccc
     4681 caggctggct acaagaacta cgcggtgaac atgtgccaga aaccgaagaa tacggcattt
     4741 ctagctttgt ctattgcgcg cgccagccat ttcatccgca gcgtttatgg cattggttta
     4801 atcaagaatg ggcaggcgtt atacgctcaa aaggccattt ttggatagcc agccgcccag
     4861 agtattgttt gaactggtca caagcggggg caattacacg cacagaacct gctggctttt
     4921 ggtgggcagc ctcccctaaa aattactggc ctgaagacaa agaacaactg caaatgattc
     4981 aagaccgctg gcaagaaccc tatggtgaca gacagcaaga aattgtgatt ataggcatgc
     5041 aaatgaaccg tgacgcgatt attgctggct ttgatgcgtg tttgctaaac gcgcaagaaa
     5101 tagcactcgg tatggacggc tggaaactat tagatgaccc atttccaaag tggaatgcca
     5161 ttcatgatga agctgatgaa gttaattaat tttaagataa aatacgcaca tggacacacg
     5221 cattcccgtc accttactca ctggcttttt aggttcaggc aaaacgacac tactcaataa
     5281 actattagca aacccacgca tgaaagacag cgcggtaatt attaacgagc tgggtagcac
     5341 gaggctagac catattctgg ccacgcaagt ggagagtgag catattgccg ataacacggt
     5401 gcttttagaa tctggctgca tctgttgctc gctcaaaaat gagctggccg ataccatgcg
     5461 cgatttgttt tttaagcgtg cgttgcaggc gattccgcaa tttaatcgct tagtgattga
     5521 aaccactggc atggccgacc ctgggccgat tttgggcaat ctaatgaacg agccggtgat
     5581 agaaagtgtg tttcggttag atgcagtggt ggtgacggtg gatagcgctt atggcttaaa
     5641 acagttgcaa gaacacttgg aagcgcgcaa acaagcggca gtggccgatg tgttgctact
     5701 caccaaaacc gatttagcca gtgcagatga agtgaacgca ctcaaagaaa agttgattga
     5761 aattaacccc ggtgccaccc agcacaagat tgtgcatggc gacatagacc ccgattttat
     5821 tattgatgtc ggcctgtttg accccactgg caaacgtgcc gaacctgaac gctggttgcg
     5881 cgcgccgtta aaagttgcgc gccctaaagg cacgctgcca caagccagcc agcacgatga
     5941 aatcagcagt tttaccgtca ccctgcccag cccgcttaac tggcgcgatt taaaaccggt
     6001 gattttgaaa ctatgccaaa cgcatggaga taaacttttg cgcttaaaag gtattatcca
     6061 cgcggctgac caagccgcac ctttggcgat tcacgccgtg cattttaccc cttacccgcc
     6121 aaccttatta gaaggctgga ctgaagatga acccgaaaac cgcattgttt taatcggcaa
     6181 agggctggat gagtatgcga ttagaaagct gctaacgcag gtgtaaaatg cgccgaaaat
     6241 tagctaaatc ttttgggtat tttgtaaagc taaaaacacc cgatagaaaa caacgcctct
     6301 tgagcctcat agaaactggc ttacagaggt ggtgaaatga acttgtgcta aaaagcacgt
     6361 ttcttaatgt tttttgctta acattaaagc tcggcaaggc acttcacgtg cgtcatcaca
     6421 ctacggccaa gggcgtgcag ttcataaccg ccttcaagtg ctgaaacaat gcgtttgtgg
     6481 caaaattgct cggcgatttt tttaagctgt tgcgtgaccc ataaataatc tttttcgtgc
     6541 aaggccaagc cacccatatc gtcttctcgg tgcgcatcaa accctgctga tatcaatatc
     6601 agttccggct caaagcctgt gagtgctggc agccaacgct cttcaaccgc cgacctaaag
     6661 ccttccccac tggtgccagc ggccaacggc acgttaatga tgtgttcatt accgctctct
     6721 gccccacaat aagggtaata aggatgctga aaagtggagc acagcattac gcgtgggtca
     6781 tcacgaaaaa tatcttcagt gccatcgcaa tggtgcacat caaaatcagc aatcgccacg
     6841 cgttgcaagc cgcgatgttc aagcgcatag gccgctgcca ccgccacatt gttaaaaata
     6901 caaaagcccg atgcgccatt gcgcttagcg tggtggccag gcgggcgaat attgcaaaag
     6961 gcgttgttca cctcaccaga caacactaaa tctaccgcat taacaactgc acccgtggca
     7021 taaagcgccg catctagcgt gtatgggttc attaaagtgt cgccatctaa atctattaaa
     7081 ccatcgcttg gcgcgttttg gtaaacccaa tcaatatagt caacactgtg cgctagggcg
     7141 agtatttctt tgccaacttt tgacgcttcg tagtgattta aataatctaa tatgcctgaa
     7201 gcaatgagct ggtcggtgat ggcatacata cgcgcggggc attctgggtg gtctggcccc
     7261 atgtcgtgct taagaaattc agggtgagaa atataagctg tgcgcatttt tcacctataa
     7321 atctaaatat acatctttaa tattagtatc atcttcgttc gttttagcgc taaaactcaa
     7381 tcgtttcatc agcttgagca tgctggagtt gttttttaat acttcacctt caatgcgttt
     7441 taggccttta gagcgtgcaa tatttattaa agtggtcatc aatttatggc ctaaacctgt
     7501 gccacgcatg
//
LOCUS       DAOQSI010000104         7425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_9643_length_7425_cov_4.876798, whole genome shotgun sequence.
ACCESSION   DAOQSI010000104 DAOQSI010000000
VERSION     DAOQSI010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 7425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7425
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9643_length_7425_cov_4.876798"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1408)
                     /gene="ppsA"
                     /locus_tag="PKL42_07300"
     CDS             complement(<1..1408)
                     /gene="ppsA"
                     /locus_tag="PKL42_07300"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HNU66919.1"
                     /translation="MSSHVIPFENLRNSDVPSVGGKNASLGEMISQLNAKGVRVPTGF
                     ATTADAYREFLAQNGLDKKINDLLDNLNADDTQALAKCGTQIRAWIMDAPFPAALDKA
                     IAENYEILIAKSGNGATFAVRSSATAEDLPDASFAGQQESFLNIHGLDNILHAIKEVF
                     ASLYNDRAISYRVHKGFVHADVALSAGVQQMVRSDIGCAGVMFTIDTESGFKDVVFIT
                     SSYGLGETVVQGAVNPDEFYVHKPTLAQGKPSIVRRTMGSKLIKMVFSDATRAGKSTH
                     TIDVDAADSDRYSLSDADVLELARYATTIEAHYGCPMDIEWGKNGVDNKLYILQARPE
                     TVKSQETLNNVTETFKLNKHSEKPLVVGRAVTQKVGVGPVRIVLDPAEMHLVQPGDVL
                     VADMTDPNWEPVMKRASALVTNRGGRTCHAAIIARELGIPAIVGAVNATDVLREGEIV
                     TVSCAEGESGFVYHGAIEY"
     gene            1704..2684
                     /locus_tag="PKL42_07305"
     CDS             1704..2684
                     /locus_tag="PKL42_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HNU66920.1"
                     /translation="MSTKQFHLDSVTVSANARLHLGFFDLNGELGRKFGSLGVALSEP
                     VTRLTASKAHILQVIGAESHRAEKVLRQLIHARQLPASYGASIVIHQAISAHAGLGSG
                     TQLALAVGMAFSALYDLNLTPLEIASITSRGARSGIGIGTFLQGGVIVDGGRGSQTQI
                     PPVIARADFPTNWRIILIYDASHQGLCGEHEHHAFLQLQPAQAQVSEQLCRQVLMQAL
                     PALAEEDLPSFGRAICALQTATGDYFAPAQGGRFASLKVAQVLQFLENQGVHCVGQSS
                     WGPTGFAIVANEAQAHEVKEKLIAHFCAEKALTFQCVRGENRGAILDVSN"
     gene            2920..3834
                     /locus_tag="PKL42_07310"
     CDS             2920..3834
                     /locus_tag="PKL42_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydromethanopterin dehydrogenase"
                     /protein_id="HNU66921.1"
                     /translation="MEKVSILHLITAAKNASPFDVNMAFDAGFDNIMPYTNVTLNEIT
                     GLVQDAIFSRSPSGIKREGIFIGGRDIDLAMDMFEAAQKSMFTPFEVSVFADPSGAFT
                     TAAAMVAKVEVHLKKHFQTDLQGKHVAVFGATGPVGGCAAVIAAKQGADVTLVAHRTL
                     ADVQQIAQHLNQRYGITLRAVDGMSEASKQAILATSDIALCTAAAGVRVIQISQFLGS
                     ANLKVIADVNAVAPSGAEGVEASADGARIADTAVLGIGALAIGNLKYKTQQQLLESML
                     IRAQPNAKPNFLEFMAAFDVARALSAKV"
     gene            3821..4978
                     /locus_tag="PKL42_07315"
     CDS             3821..4978
                     /locus_tag="PKL42_07315"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNU66922.1"
                     /translation="MPKFSAEHLRGSPSLTLIVASFSARAYAQALVEAGHQVVTLDAF
                     ADADTVRIVKHTYQFRLHNFQVDVADFKRVFLTLELSQFDGFLYGSCFDALPELLDWV
                     ASKIRVIGNNAAVMRIAKGFDFFKRLDALNICHPSVQKTMPVNATQWLGKRLGGTGGL
                     HVSEVINQAQLLSSMQQVDYYQEKINGIPISMLFIANGEIAQGLGFNRQLLAPSDRWP
                     YRYGGAVSAYDLPHAAKLSFEVAAQELTQQLGLRGVNSLDAILSIDKLWILELNPRLS
                     ATFSLYPNAITAHLKSCAGELVDLPKVQGAKAHYILYADQPVEIAADFKWPDGVFDLP
                     QPLLGENTVQIAEDSPICTVEARAGNAELAHAAVLKKAQQLQLQLGMLKST"
     gene            5009..5980
                     /gene="mch"
                     /locus_tag="PKL42_07320"
     CDS             5009..5980
                     /gene="mch"
                     /locus_tag="PKL42_07320"
                     /EC_number="3.5.4.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HNU66923.1"
                     /translation="MAQHPNIQHVSVNALAAPLLQKLIKGAKSFGIEVTQLDNGCTII
                     DAGIKTEGSIRAGLLIAEICMGGLGKVSVKKSKQFEHCPLLVDVHSQTPVLACLGSQY
                     AGWALQHEKFFSLGSGPARAIAQREDLFKELNYQDSAEATMLVLETEKIPPMEVINKV
                     AQDTRVKPENLTFILTPTKSLAGTVQIVGRVLEVALHKAHTIHFPLAQIISGAGTAPI
                     PPVSGDFLTAMGRTNDAILFGGFVQLQVKSSDKEAEKLSQELPSCASKDYGRPFAEVF
                     KAVNMDFYQIDPLLFSPAKVSIKNMATGKTFTGGELNESLINKSFFS"
     gene            6027..6950
                     /locus_tag="PKL42_07325"
     CDS             6027..6950
                     /locus_tag="PKL42_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HNU66924.1"
                     /translation="MDPKTLQTPLEQVIIFTDELATQGGWHGEQLKLAFAARGVSSTF
                     ASLRDCLLDFSGLKPVIRIPQCEGVPQAAFVRGIAGGSMQQVTTRLNVLHLLKRLGTF
                     VYNDAHAIERTVDKAMTSFLLLQAGISTPPTWVCESRQHAHQTIRQHLKSHAQIVIKP
                     LFGSQGQGVRLLHQKTPWPLPMDNFVDGVFYLQTFVEATHDYRVFVMNHQAKVAMRRT
                     GEHWLHNVALGAKCERIIDLDVLNIATQAAIALDMAYCGVDVIRNKAGELQVLEVNSI
                     PAWRGLQSVCEINIAELLVDDLLRQLNDTKV"
     gene            6988..>7425
                     /locus_tag="PKL42_07330"
     CDS             6988..>7425
                     /locus_tag="PKL42_07330"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HNU66925.1"
                     /translation="MTPEKIANAYKLACMAELEALKPGNVHIFSDGHGMQISDFMLSA
                     EASALVIAQPNLTVGERILSAVQATQEAVGLNTNLGIILLCAPMIQAALQLKSEVNFN
                     HEALQAALQDCLHHLSVIDAEDTSSAIVLANPAGLGEVSQYDVH"
BASE COUNT         1950 a         1568 c         1827 g         2080 t
ORIGIN      
        1 cgtattcaat tgcaccgtgg taaacaaagc ctgactcgcc ttcagcgcag gatacggtaa
       61 cgatttcgcc ttcacggagt acgtcagttg cgttgaccgc acccacaatc gcaggaatac
      121 ctaattcacg cgcgataatt gcggcatggc aagtgcgacc gccacggttg gttacgagtg
      181 cagaagcacg tttcatcacc ggttcccagt ttgggtcggt catatcggct accagcacgt
      241 cacctggttg tactaaatgc atttcagccg ggtcgagtac gatgcgcaca gggccaacgc
      301 caactttttg agtcactgcg cggccgacta caaggggttt ttcgctatgt ttgttcagtt
      361 taaacgtttc agtaacgtta ttgagtgttt cttgcgattt cactgtttct ggacgggctt
      421 gcaaaatgta gagtttgtta tctacgccgt ttttacccca ttcgatatcc atcgggcagc
      481 cgtaatgcgc ttcaattgtt gtcgcgtaac gcgctagctc cagcacatcg gcatcggata
      541 atgagtagcg gtcgctatca gccgcgtcca catcaatggt gtgcgtactt ttaccggcac
      601 gtgtggcatc actgaagacc attttaatga gtttagagcc catggtgcgg cgcacgatag
      661 agggttttcc ttgcgccaaa gttggtttat gtacgtagaa ttcatctggg ttgactgcgc
      721 cttgcaccac ggtttcacct aggccgtaac ttgaggtaat gaataccacg tctttaaagc
      781 cagattcagt atcaatggta aacatcacgc ctgcacagcc aatatcagag cgcaccattt
      841 gttgtacgcc tgctgaaagt gcgacatccg catgcacaaa gcctttatgc acacggtatg
      901 aaattgcgcg gtcgttatac aaagaagcaa acacttcttt aatggcatgc agaatgttat
      961 ctaacccatg aatgtttaaa aatgattctt gctgaccagc aaatgaagca tctggcaaat
     1021 cttcagccgt ggcagaagag cgcactgcaa acgttgcgcc attgcccgat tttgcaatga
     1081 ggatttcata gttttcagcg atggctttat ctaatgccgc cgggaagggc gcatccataa
     1141 tccaagcacg aatttgtgtg ccacattttg ctaatgcttg cgtatcatct gcgttaagat
     1201 tgtctaataa gtcgttgatt tttttatcta aaccattttg cgcaagaaac tctcgataag
     1261 catcggcagt ggtggcaaac cctgttggca cacgcacgcc ttttgcgttt aattgagaaa
     1321 tcatttcacc taatgaagca tttttaccgc caactgaagg cacgtcgcta ttgcgtaggt
     1381 tttcgaatgg aatgacatgg ctggacataa ctaacccctt aaatttgcgc tgaatgaaac
     1441 ttacatttaa atcaagtttg ctttgaaact aactactaac cgcaatgtat aaacccaaat
     1501 gaacaaaccc tagtaatcag gctcatgtgg aattaaaaat cagttttaaa gttttatttg
     1561 aattatggtt taaactctta tgagtcatta tgccaaaata gacaagtgaa gtcacgctga
     1621 acttcacgag gtcggatagc ctattgaggc taacaaatcg gtattttcag cgattattta
     1681 aatttattga tagactttga tttatgtcaa caaagcaatt tcatctagat agtgtgacgg
     1741 taagtgcaaa tgcaaggctg catttgggtt tttttgattt gaatggcgag ctagggcgca
     1801 agtttggtag ccttggtgtg gcgctttccg aacccgttac acggttgact gcaagcaagg
     1861 ctcacattct tcaggtaatc ggtgctgaat cgcatcgtgc agaaaaagtg ttaaggcaac
     1921 tcatccatgc gcgccagttg ccagcttctt atggcgcatc gattgtgatt catcaagcca
     1981 tttcagcgca cgcagggcta ggctctggca cgcaattggc gttagcggtg ggaatggcgt
     2041 ttagtgcgtt gtatgactta aaccttacac cgttagagat tgccagtatt acttcacgcg
     2101 gtgcgcgctc tggcataggg attggtacct ttttacaagg gggcgtgatt gtggatggcg
     2161 gtcgcgggtc gcaaacgcaa attccacctg taatagcaag ggccgatttt ccaacaaact
     2221 ggcgtattat tcttatctat gatgcctcgc accaagggtt atgtggtgag catgagcatc
     2281 atgcatttct tcaattgcaa cctgcacaag ctcaagtttc tgaacaatta tgccgacaag
     2341 tattaatgca agcgctaccc gcgttggctg aagaagattt gccaagtttt ggccgtgcga
     2401 tttgtgcctt acaaacagca acaggtgact actttgcacc agcgcaaggg ggccgatttg
     2461 ccagtcttaa agttgcgcaa gtattgcagt ttttagaaaa ccaaggcgtg cattgcgttg
     2521 ggcaaagttc atggggacca acaggctttg ccattgttgc caatgaagca caggcgcatg
     2581 aagtaaaaga aaaacttatc gcgcactttt gcgctgaaaa agcactcaca tttcagtgtg
     2641 tacggggcga aaatcgtggt gcaattttgg atgtttctaa ttagtaaaaa aacaggaaat
     2701 tacatgcttt ttaacaccaa acctttttcg atgctctgcg agcctttatt tacaaggctt
     2761 ccagagcata ttttttcgct cggagaattt taagctggtt ttagtgttta aattcacaag
     2821 ttgtatggtg gttttttatc catattattt gggattatcg attgttaaat ggccggcaat
     2881 tcagtaagct gattattcat gtgttgaaag tgctttgaaa tggaaaaagt ttcgatatta
     2941 catttaatca cggcggccaa aaatgccagc ccgtttgacg tgaacatggc atttgatgct
     3001 ggctttgaca acatcatgcc ttacacaaac gtcacattaa atgaaatcac cggtttggta
     3061 caggatgcga ttttttcgcg cagcccatcg ggcattaagc gcgagggcat ttttattggc
     3121 gggcgtgaca ttgatcttgc catggacatg tttgaagccg ctcaaaaatc catgtttacc
     3181 ccttttgagg tttcagtgtt tgcagaccct tctggcgcgt ttaccacagc ggcggcaatg
     3241 gtggctaagg ttgaagttca tcttaaaaag cattttcaaa cagatttgca gggcaagcac
     3301 gttgccgttt ttggcgcgac tgggccggtg ggtggttgcg cggcggtgat tgcagccaag
     3361 caaggtgcgg atgtgacgct ggtggcacat agaacgctgg ccgatgtaca gcaaattgcg
     3421 cagcacttaa atcaacgtta cggtatcact ttgcgtgcag ttgatggaat gtccgaggca
     3481 agcaagcaag ccattttggc aacatcggat attgcgcttt gtacggcggc tgctggtgtt
     3541 cgtgttattc agatttcaca gtttttgggt tctgccaatt taaaagtcat tgccgatgtg
     3601 aatgcggtag cgccatctgg cgctgagggc gtggaggcaa gcgcggatgg cgcgcgtatt
     3661 gcagacacgg ctgtgctggg aattggcgca cttgcgattg gaaatttaaa atataaaacg
     3721 cagcagcaat tacttgaatc catgctgatt agagcgcaac ctaatgccaa acccaacttt
     3781 ttggaattta tggcggcttt tgatgtggcg cgcgccctga gtgccaaagt ttagtgcgga
     3841 acatttacgg ggtagcccat ccctaacctt gattgtggca agtttttcag cgcgtgccta
     3901 tgctcaggca ctggttgaag ctggccatca agtggtcaca ctagatgcct ttgctgatgc
     3961 agatactgta cgtattgtga aacataccta ccagtttcga ttgcacaatt ttcaagtaga
     4021 tgtggcagat tttaagcgcg tatttttaac cctcgaactt tcacagtttg atggtttttt
     4081 atacggcagc tgttttgatg cactgccaga gttgcttgat tgggttgcat ctaaaattcg
     4141 ggtcatcggt aacaatgcgg cggtcatgcg catagcaaaa gggtttgatt tctttaagcg
     4201 cctcgatgcg ctaaatattt gccatccttc ggtacaaaag actatgccag ttaacgcaac
     4261 ccaatggctg ggcaagcgct tgggcggcac gggtggcctg catgtgagcg aggtaatcaa
     4321 tcaggcgcaa ttgttaagct caatgcaaca ggttgattat tatcaagaaa aaattaacgg
     4381 aatacccatc tcaatgctgt ttatcgcgaa tggtgaaata gcgcaagggc ttggatttaa
     4441 tcggcaatta cttgcaccca gtgaccgttg gccttatcgt tatggcgggg cagttagtgc
     4501 atatgattta ccacatgcgg cgaaactttc atttgaggtt gcggcgcagg aattaacgca
     4561 gcaattgggc ttacgcggtg tcaatagttt agatgcaatt ttaagcattg ataagctatg
     4621 gattttagag cttaatccac gtttaagcgc cacgttttca ctgtatccta atgcaatcac
     4681 agcgcattta aaatcttgcg ctggcgagct ggttgatttg ccaaaagtgc agggggcgaa
     4741 ggcgcactat atcctctatg cagaccaacc agtagaaatt gctgcggatt tcaagtggcc
     4801 tgatggcgtg tttgatctac cgcaaccgtt gcttggggaa aacactgtgc aaatcgcaga
     4861 agattcgccc atttgcacgg ttgaagctag ggcaggcaat gctgaattag cccacgcggc
     4921 agtgttgaaa aaggcgcaac aactgcagtt gcaattgggt atgttaaaga gcacgtaatt
     4981 tgcaaagata ctgataagga cttaaagaat ggcacagcat cccaatattc aacatgtcag
     5041 cgttaatgca ttagcagcgc ctttgctaca aaaattgata aaaggtgcca agtcttttgg
     5101 catagaagtg acgcaactag ataatggctg caccattatt gatgcgggca ttaagactga
     5161 gggcagtatt cgcgcgggtt tgttgattgc tgagatttgc atgggcggct tgggtaaagt
     5221 aagcgttaaa aaaagtaaac aattcgagca ttgtccattg ctggtggatg tgcattctca
     5281 aacaccggtg ctggcttgct taggtagcca atacgctggt tgggcgttgc agcatgagaa
     5341 gtttttttca cttggcagcg gcccagcacg tgcaattgcg caaagagaag acttattcaa
     5401 ggagttaaat tatcaggata gtgccgaagc taccatgctg gtgcttgaaa ctgaaaagat
     5461 tcctccaatg gaagtgatta ataaggtcgc acaagatacc cgtgttaaac ctgaaaactt
     5521 aacttttatc ctgacaccta ccaaaagctt agcgggtacc gtgcaaatcg tcggccgcgt
     5581 gttagaggtg gcgctacata aggcacacac gattcatttt ccgctcgcgc aaattatcag
     5641 tggggctggc actgcgccca ttccacctgt gagtggtgat tttttaacgg ccatgggccg
     5701 caccaatgat gcgattcttt ttggagggtt tgtgcagttg caggtgaagt cgagtgataa
     5761 agaggcggaa aaattatccc aagagttgcc aagctgcgct tcaaaagatt atggcaggcc
     5821 ttttgcggaa gtgttcaaag cagtcaatat ggatttttac caaattgacc cattattatt
     5881 ttcacccgca aaagtgagta tcaaaaatat ggccaccggc aaaacattta ccggcggtga
     5941 gttaaatgaa tcactcatca ataaatcatt ttttagttaa attaaaaggt tataatccat
     6001 attcaatgtt atttattgaa tagtcaatgg atcctaaaac acttcaaaca cctctcgagc
     6061 aagtgatcat ttttacggat gagcttgcaa cgcaaggtgg ctggcatggc gagcaactta
     6121 agttggcctt tgcagcccga ggtgtttctt caacctttgc ttccctcaga gattgcctgc
     6181 ttgatttttc gggactaaag cctgtcattc gcattcctca atgtgaaggc gttccgcaag
     6241 ccgcatttgt gcgcggtatt gctggcggta gcatgcaaca agtgaccacc agactgaacg
     6301 tactacactt gttaaaacgc ttgggcacgt ttgtatataa cgatgcccat gccattgagc
     6361 gcaccgttga taaagccatg acgagctttt tattgttaca ggcaggtatt tcaacgccgc
     6421 ccacttgggt gtgcgaatcg cgtcaacatg cgcatcagac catccggcaa cacttaaaaa
     6481 gtcatgcgca gattgtaatt aagccattat ttggctcgca agggcagggg gtgcgcctat
     6541 tacatcaaaa aactccatgg cctttgccga tggataattt tgtagatggg gtgttttatc
     6601 tgcaaacttt tgttgaagca actcacgatt atcgcgtttt tgtcatgaat catcaggcaa
     6661 aggtggcgat gcgtcgtacc ggggaacatt ggttgcataa tgtggcgtta ggcgcaaaat
     6721 gtgagcgtat tattgacctg gatgtattga acattgccac gcaggccgcc attgcgcttg
     6781 atatggccta ttgtggtgta gatgtgatac gtaacaaagc aggcgaactg caagtattgg
     6841 aggtcaatag tattcccgca tggcgaggct tgcaaagcgt ttgtgaaatc aatattgctg
     6901 aattattagt tgatgattta ttgcgtcaat tgaatgacac taaagtttaa ttaaccacag
     6961 tttaatcgca ccacttttta gcacatcatg accccagaaa aaattgccaa tgcttataaa
     7021 cttgcttgca tggctgagct tgaagccctc aagccaggga atgtgcatat tttttctgat
     7081 ggtcatggta tgcagattag tgacttcatg ctgagcgctg aggcgtctgc tttagtgatt
     7141 gcacagccca atttaacagt gggcgagcgt attttatctg cagtgcaagc aacgcaagag
     7201 gcggttggtt taaataccaa tctcggcatt attttactat gtgcgccaat gattcaggcg
     7261 gccttacagt taaagagcga agtaaatttt aaccatgagg cgttgcaggc cgcgctacaa
     7321 gattgtctac atcatttaag tgttatagat gcagaagaca cgtcaagcgc aattgtttta
     7381 gctaacccgg caggcttggg tgaagtgagc cagtatgatg tgcat
//
LOCUS       DAOQSI010000105         7414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_9663_length_7414_cov_5.577796, whole genome shotgun sequence.
ACCESSION   DAOQSI010000105 DAOQSI010000000
VERSION     DAOQSI010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 7414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7414
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9663_length_7414_cov_5.577796"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2066
                     /locus_tag="PKL42_07335"
     CDS             <1..2066
                     /locus_tag="PKL42_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNU66926.1"
                     /translation="ILFLFLGEVRSALVVVAALPLAMLAAFICMDQAGLSANLMSLAG
                     LAIGIGMMVDGAVVMVENSFRIMAERLEHGGKVNRTEAVLTAAKEVANPMAFAILIII
                     VVFLPLFSLQGLEGKMFKPMAFSISFAMAGSLILALTLIPVLAALFLKPKAERDTLLM
                     RVIKRRYLPLLDWVLSHKKAVGIISGVLLVASLALFPFLGKEFMPQLQEGSIMWRVTS
                     IPSTSLDESIAISKRIEKSLKEFPEVNTAIAMIGRAEKGETADVNYMEIYTELKPKEA
                     WTNGQSIEQLADGMREKLEKVAPNSVIAFTQPIQMRVEELISGVRATLALKVYGGDLA
                     TLDRLSGQLKEVVDKVPGATDVSLEANIGKPQIRITVDREKLARYGMNADDVLQVVRS
                     GIGQEPISTLIDGVRRYDIAFKLEDSAKASPAAIGAIPLRTPTGGMIPLAEVAKVDVA
                     EGYSFIRREQLQRYAVIQLDVKGRDVDGFVKEAEANISQKVKLPTGYWIEWGGAFENQ
                     QRALTRLSIIVPLTIFFIFILLYTAFNSFKYAAMILANVPFAAVGGILALFITGQYLS
                     VPSAIGFIAVFGVAMLNGIVLVSFFNELQDKGLSVAEAVKQGAMLRLRPVLMTASVAI
                     LGLVPMLLSSGVGAETQRPLATVVVGGLITSTLLTLLLLPVVYEWVETRASRKANQTT
                     SELNT"
     gene            2082..2420
                     /locus_tag="PKL42_07340"
     CDS             2082..2420
                     /locus_tag="PKL42_07340"
                     /inference="COORDINATES: protein motif:HMM:NF012753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNU66927.1"
                     /translation="MKEIKAFIHAHRVSDVLQAIKEVDSSGVLISNFAVFPVQALLHD
                     TELKEARYSVNFGETVINEFKIEILCHEEAIASLVEVIQRVGHTGDHEEGWIIVTDVL
                     SAVPVGKIHK"
     gene            2429..3151
                     /locus_tag="PKL42_07345"
     CDS             2429..3151
                     /locus_tag="PKL42_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNU66928.1"
                     /translation="MQTAQSVILFALYPAAAVMAGGAIALWRTMPPRLISAVQHFAAG
                     VLFCALSTELLPDLVHRKMPWVTLLGFTLGVIVMLLVKHFAENFGQRGLIKSQQPISL
                     IVILGLDIALDGLLIGLGFAAGQKQGLLLTIALTLEVLFLGLSGGAALQSSGASRKRV
                     LFITLGFALTLISGAWAGQTLLAGASDLLLDAVLAFGLAALLYLVTEELLVEAHEVPE
                     TSAQTAMFFVGFILLLTIEMFI"
     gene            3179..5590
                     /locus_tag="PKL42_07350"
     CDS             3179..5590
                     /locus_tag="PKL42_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNU66929.1"
                     /translation="MSDNHNHNSDLPLKAETATKILSETLILHIEKMDCPTEESLIRK
                     KLEGMKGVESLDFNLIQRKLTVRHRLPDPEPIISAVVALDMEPVIENAAKPLSRSSQA
                     IFIIDNMDCPTEEALIRNKLSTMNGITELHFNLMQRKLTVTHTLGKSDAIKQALITID
                     MHAVPLVATAVQAQSDNAPPPNSQKKWWFLGFGGVAAILAEVIAWTGGTDKSWPVVAL
                     ALFAIAVSGTSTYKKGWIALKNLNLNINALMAIAVTGAVLIGQWPEAAMVMVLFTLAE
                     MIEAMSLDRARNAIRGLMEMTPDKANVLQANGTWKELEAATITIGMTARVAPGERIPL
                     DGELTKGQSAVNQAPITGESIPVAKAIGDKVFAGTINETGSFEYRITAVQTDSTLSRI
                     IRAVEDAQGSRAPTQRFVDSFAKIYTPIVFVVALGIAVFPPLLFGLAWMPWIYKALVL
                     LVIACPCALVVSTPVTVVSGLAAAAKAGILIKGGVYLEEGRKLKSLALDKTGTITQGK
                     PVVTDIVSLSGEKSQAMRLAATLAGRSDHPVSAALSNHFKSQSTLVLEDVTDFEAITG
                     RGVKGRIGTQLYYLGNHRLVEELGICNGATEAALNKLEADGKTAVIISNDTTPLAVIA
                     VADTVRESSRQAIVELHALGIRTLMLTGDNDLTAKAIAKSVGIDDARGNLLPEDKLSV
                     INEELSKYGTVGMVGDGINDAPALAKSSIGFAMGAAGTDTALETADVALMDDDLRKIP
                     HFIRLSIKAAGILKQNIIIALSIKAVFLVLAFMGVATLWMAVFADMGASLIVVFNGLR
                     LLRRG"
     gene            5644..6579
                     /locus_tag="PKL42_07355"
     CDS             5644..6579
                     /locus_tag="PKL42_07355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNU66930.1"
                     /translation="MSGSHAHGAGISSNKMWFATAITFAFCLGEALIGYKSNSLALMA
                     DAGHNFADSLALALSAFALWVATKPADNKRTFGYHRVGILAALVNAVGLAVMAVVIFW
                     EAIQRLQTPEHVQSGPMIWVALLAIILNSGIAWWLSSAAKEDLNIRSAYLHMLGDAAA
                     SLGVVIAGIIIAFTSWFIADPIVSIIFALLVLWSSWSIFTESIQMLLEGIPVGMELNQ
                     VEQAIREVKGVLDVHDLHVWTVSSGLIACNCHILVAEQNVSAAQKIQAEVAHELEHDF
                     KISHSTIQIEVEDCGGHDHTTQSDHEHEHKHKHGH"
     gene            6641..7048
                     /locus_tag="PKL42_07360"
     CDS             6641..7048
                     /locus_tag="PKL42_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66931.1"
                     /translation="MRLLLISGLAIVLISCTHDIALQTPDGQPVGKAVLAFEGNSSGT
                     IAIDRNGEAYRGQWIASKVDESAKIASAYGIGSKKYKDYKRGWGKYLRAGQSRLQSDK
                     GGILNCEFQYRGTNIHGSCKSETEEFEFIGKPE"
     gene            7056..7175
                     /locus_tag="PKL42_07365"
                     /pseudo
     CDS             7056..7175
                     /locus_tag="PKL42_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611385.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2034 a         1468 c         1810 g         2102 t
ORIGIN      
        1 tcattttgtt cttattctta ggtgaagtgc gttcagcatt agtggtggtc gcggctttac
       61 cattggcgat gttagctgct ttcatttgta tggatcaggc tggactctcg gctaacttga
      121 tgtcgcttgc gggtcttgcg atcggcatcg gtatgatggt ggacggcgct gtggtgatgg
      181 ttgaaaactc tttccgtatc atggcagagc ggcttgagca tggtggcaaa gttaaccgaa
      241 ctgaagctgt cttaacagca gcaaaagaag tggctaaccc aatggcattc gctattctca
      301 ttattatcgt cgtgttctta ccactcttca gcttacaagg actggaaggc aagatgttta
      361 agccgatggc ttttagtatc agctttgcga tggctggttc gttgatactt gcattaacgc
      421 taatacccgt gcttgctgca ttattcctga agccaaaagc agaacgggat acgctgttaa
      481 tgcgcgtcat caaacgtcgt tacctaccac ttttggattg ggtgctgtca cataaaaaag
      541 ctgtcggcat tatttccgga gtgttgctgg tagctagtct tgccttgttc ccatttctgg
      601 gtaaagagtt tatgccacaa ctgcaagaag gctcgatcat gtggcgcgtg acttctattc
      661 cgtctacttc gctggatgag tcaatcgcca tttccaaacg cattgaaaaa tcactcaaag
      721 agttccctga agtgaatacc gctatcgcca tgatcggtcg cgctgaaaaa ggtgaaacag
      781 ccgatgtcaa ttacatggaa atctacaccg agctcaagcc aaaggaggca tggacgaatg
      841 gtcagtcgat tgaacagttg gctgatggta tgcgagagaa attagaaaaa gtagcaccca
      901 actcagtgat cgcctttacc caaccaattc agatgcgcgt agaagagttg atttctggtg
      961 ttcgagcaac gttggcctta aaagtctatg gtggcgactt agctacttta gataggctat
     1021 cagggcaatt aaaagaagtg gtcgataaag tgccaggtgc taccgatgtg tcattagaag
     1081 ccaatatcgg taaaccacaa atccgtatta cggttgatcg agagaagctt gctcgctatg
     1141 gcatgaacgc ggacgatgta ttacaagtag tgcgttctgg tatcggccaa gaacccatct
     1201 ctacattgat tgacggcgtg cgccgttacg atattgcgtt caagctggaa gacagcgcaa
     1261 aagcgagtcc agctgcaatt ggcgcaattc cgctacgcac gcctacaggc ggcatgattc
     1321 cgcttgcaga agtcgcaaaa gtggatgtgg ctgaaggcta ttcctttatt cgtcgtgagc
     1381 agttacagcg ttacgccgtg attcaattag atgtgaaagg tcgtgatgta gatggttttg
     1441 taaaagaagc agaagctaat atcagtcaga aagtcaaact accgaccggc tactggattg
     1501 aatggggcgg cgcttttgag aatcagcaac gggcactgac tcgactgtcc atcatcgtgc
     1561 ccctgacgat tttcttcatc tttattttgc tttataccgc gttcaattcg ttcaaatatg
     1621 cggcaatgat tttagccaat gtgccctttg cagcagtggg tggaatattg gcattgttca
     1681 tcactgggca atacctatcc gtaccatccg ctattggttt tattgccgtg ttcggtgtgg
     1741 cgatgttgaa tggtatcgtt ttagtgagct tctttaatga gttgcaggat aaaggtttat
     1801 cggttgcaga ggcagttaaa caaggtgcga tgttgcggct aaggccagta ctgatgactg
     1861 ccagcgtagc gatactaggt ttagtaccga tgttgctttc tagtggtgta ggcgctgaga
     1921 cgcaacggcc acttgctacc gtggtagttg gtggcttgat tacatcgacg ttacttactt
     1981 tactgttatt gcctgtagtt tatgagtggg ttgagacgag agcatctcgc aaagccaatc
     2041 aaactacatc tgaattaaat acttagtata ggggatcaaa aatgaaagaa attaaagcgt
     2101 ttatccatgc tcatcgcgtt tcggatgtat tgcaagcaat aaaggaagtt gatagcagtg
     2161 gagtgctaat tagcaacttt gctgtattcc ccgtacaagc gctacttcat gacacagaat
     2221 tgaaagaagc caggtactcg gtaaatttcg gtgaaacggt gattaatgaa tttaagatcg
     2281 aaatcttatg tcatgaagaa gccattgcaa gcctagtaga agtcatacaa cgcgttggcc
     2341 atactggcga tcacgaagaa ggctggatca tcgtcactga tgtattgtcg gctgtacctg
     2401 ttggcaaaat tcacaaataa gggtgggtat gcaaactgct caaagtgtaa tactttttgc
     2461 gctctatcct gccgctgctg taatggcagg aggagccatt gcgctttggc gcacgatgcc
     2521 accgagattg atcagtgcag tgcagcattt tgcagctgga gtgctgttct gcgcgctttc
     2581 aaccgagctt ttaccggatc ttgtgcatcg taagatgcca tgggtgactt tgctgggttt
     2641 cacacttggc gttatcgtga tgctattggt taagcatttt gccgaaaatt tcgggcagag
     2701 aggattaata aaatcccaac agccaatcag tcttattgtg atattgggtt tagatatcgc
     2761 attggatggt ctgcttattg ggcttggttt cgcagcaggt caaaaacaag gattattgct
     2821 aacgattgca ttaacccttg aagtgttatt tttaggttta tcaggtggcg ctgcactcca
     2881 aagctcaggt gcaagtcgta aacgcgtact ttttatcacg ttaggatttg cccttacgct
     2941 aatctcaggc gcttgggcag gtcaaacctt actcgccggt gcttcagatc tgctccttga
     3001 tgctgtgctc gcttttggtt tggcggcact tctctatctt gtaactgaag agcttttagt
     3061 agaagcacac gaagtacctg aaacgagtgc acagacggcg atgttttttg ttggcttcat
     3121 tttgctatta acgattgaaa tgtttattta atgttaccaa gaataaggtt gcaacatcat
     3181 gagtgataat cataaccata attccgactt gccattaaaa gctgagacag ctacaaaaat
     3241 actgtctgag acactaatac tccacattga aaaaatggat tgtccgactg aggagtcctt
     3301 aatacgcaaa aagcttgaag gtatgaaagg tgttgaaagc cttgatttca atctaatcca
     3361 acgcaagctg actgtaagac atcgtttgcc tgacccagaa ccaattattt ccgcagtcgt
     3421 ggcacttgat atggagcctg tcatcgagaa tgctgctaag cctctatcaa gaagttccca
     3481 agctattttt atcatcgaca acatggactg cccaaccgaa gaagcattga ttcgcaataa
     3541 gttatccacg atgaacggta ttaccgagtt acatttcaat ctaatgcaac gtaagcttac
     3601 ggtcactcat actttaggaa agtctgatgc catcaagcaa gcattaatta cgatagacat
     3661 gcatgcagta ccattagtag caacagcagt acaagcgcaa tcagataacg cgcctcctcc
     3721 aaattctcag aaaaaatggt ggttcttagg ctttggtggc gtcgcagcta ttttagccga
     3781 agtgattgca tggacaggtg gtaccgataa atcttggcct gtagtggctt tagcgctttt
     3841 tgcgattgca gtgagtggta ccagcaccta taaaaaaggc tggattgcgc ttaagaatct
     3901 taatctgaat atcaatgcat tgatggcgat tgctgtaaca ggtgcagttt taattgggca
     3961 gtggccagag gcagccatgg ttatggtgct ttttacactt gctgagatga ttgaagccat
     4021 gtcgctggac cgtgcacgta atgctattcg tggcttgatg gaaatgacgc ctgacaaggc
     4081 aaacgtttta caggctaatg gcacttggaa agaattagaa gccgcaacaa ttaccattgg
     4141 catgactgcc cgtgtagcgc caggcgaacg cattccatta gatggggaat tgactaaagg
     4201 gcagtctgca gtgaatcagg cgccaattac cggtgaaagc ataccggtag ccaaagctat
     4261 aggtgacaaa gtgtttgctg gcaccattaa tgaaactggc tcttttgaat atcgtatcac
     4321 tgcagtacaa accgattcaa cattgtctcg catcatacgc gcagtagaag acgcgcaagg
     4381 cagtcgcgca cccacgcaac gttttgtgga cagcttcgcc aaaatatata cacctatcgt
     4441 gttcgttgtg gccttgggga ttgcagtttt tccaccactt ttattcggat tggcatggat
     4501 gccatggatt tataaggcac tggtgttatt agtcattgct tgcccatgtg cactagttgt
     4561 atcgacacca gtcacagtag tgagtggtct cgcagctgct gcaaaagctg gaattctgat
     4621 caagggtggt gtctatctag aagagggtcg taaactcaaa tcattagcat tggataaaac
     4681 aggtacgatt acacaaggta agccggttgt aaccgacata gtgtctttga gtggcgaaaa
     4741 gagtcaggca atgagattag cggctacgtt agctggtcgc tcagatcacc ccgtgtcagc
     4801 ggcactcagt aatcatttta aatcgcaatc cacattggtt ttagaagatg ttactgactt
     4861 tgaagctatt actggacgtg gcgttaaagg acgcataggc acacagttgt attacttagg
     4921 taatcatcgt ttggttgaag aattaggcat atgcaatggg gctacagaag ccgcattgaa
     4981 taaactggaa gccgatggta aaacagctgt cattataagt aatgatacaa caccactcgc
     5041 tgtgattgct gtagccgaca cagttcgaga gtctagtcgt caagcgattg ttgaattaca
     5101 tgccttaggg attcgcacct taatgcttac cggagataat gacctcacag caaaagcaat
     5161 tgctaaaagc gttggcatag atgatgccag aggtaattta ttgcctgaag ataagctatc
     5221 tgtcattaat gaagagttaa gcaaatatgg cacggtagga atggtaggtg acggtatcaa
     5281 cgatgcgcct gcgctagcta aatccagtat tggttttgca atgggagctg cgggtactga
     5341 tactgcgtta gaaacagccg atgtcgcttt aatggatgat gatttacgaa aaattccaca
     5401 tttcattcga ttaagtatta aagcagctgg tattttgaaa caaaatatta tcattgcctt
     5461 aagtatcaag gcggtgtttt tggtactcgc ttttatgggc gttgcgacac tatggatggc
     5521 ggtattcgcc gatatgggtg caagtctgat tgtagtattt aatggtttgc gcctgttgag
     5581 acgaggctaa ataacgatgc caaattcaca caaaaacgtt aaatttttta aaaggaatta
     5641 aaaatgtcag gttcacatgc acacggcgca ggaatatcca gtaataaaat gtggttcgct
     5701 accgcaatca cattcgcctt ttgtttaggc gaagcgctca ttggatacaa atctaacagc
     5761 ctcgcactca tggcggatgc tggtcataac tttgctgata gcttggcgtt ggcattatct
     5821 gcatttgcgt tatgggtggc caccaaacca gcagataata aacgaacttt tggttatcac
     5881 cgtgtaggta ttcttgcagc actagttaat gcagttggtt tagccgtcat ggcagttgta
     5941 attttttggg aagcaattca gcgcttacaa actccggagc atgtgcaaag cggcccaatg
     6001 atatgggtgg cgctactagc aattatcttg aattctggca ttgcgtggtg gttgtcatcc
     6061 gccgcaaaag aagacttgaa tatccgtagc gcttatttac atatgttagg tgatgctgct
     6121 gcttcattgg gtgtagtgat tgcaggcatt atcattgcat tcacaagctg gtttattgcc
     6181 gaccctatcg tttccattat ttttgcactg ctcgttttat ggagctcatg gagcatcttt
     6241 actgaatcta ttcagatgct tttggaaggc attcccgttg gcatggagtt gaatcaagtg
     6301 gaacaagcga ttcgagaagt aaaaggtgtt ctggatgtac atgatttaca tgtatggacc
     6361 gtttcatcag gattgattgc atgtaattgt catatcctag ttgctgaaca aaatgtaagt
     6421 gctgcccaga agattcaagc agaagtggca catgagttag aacatgattt caagataagt
     6481 cattcaacga ttcaaattga ggttgaagat tgtggtggac atgatcatac aacgcaaagt
     6541 gaccatgagc acgaacataa acacaaacat ggtcattaac ttatgacact agatgaaaat
     6601 taaatagact ttaaattact taaccaaaaa aggatttttt atgcgcttat tacttatctc
     6661 tggattagca atagtattga tcagctgcac ccacgatatt gcactacaaa cccctgatgg
     6721 ccagcctgtt gggaaggcgg tgttagcatt tgagggcaac agttcaggca ccattgctat
     6781 tgaccgaaat ggcgaagcct accgaggtca atggatagca tcaaaagtcg atgaaagcgc
     6841 aaaaatcgct agcgcctatg gcataggtag taaaaagtac aaagactata agcgaggatg
     6901 ggggaagtat cttcgggctg gacaaagcag attacaatca gataagggcg gtattttaaa
     6961 ctgtgaattc cagtatcgtg gcactaatat acatgggtca tgcaagtcgg aaacggaaga
     7021 gttcgaattt ataggcaagc ctgagtgaat atctacgttt aaacgtgttt taatacatcg
     7081 aaatgtttta cattaggaag cgtcgacata gtcatcttga tcaactgagt ccgatggtgt
     7141 ttgaaaagtt acaagtgtgg actgcctacg gttaggtaga cacatagcct tgttcaaagt
     7201 aatttgtgca ttcacaaact agacttaatt gaattgctgg gattttttgt tccgcaaaaa
     7261 tagaaggagc cgcgtgctta ctgggtgtaa atagtattaa gtacgcgctt ttgcggaact
     7321 gaaatggtct tgtaagccaa ttcaatgctg gatattgggt atggactttt aatccgtttg
     7381 tcgtgggttc gatccccgca cgccctacca atac
//
LOCUS       DAOQSI010000106         7381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_9747_length_7381_cov_6.200655, whole genome shotgun sequence.
ACCESSION   DAOQSI010000106 DAOQSI010000000
VERSION     DAOQSI010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 7381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7381
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9747_length_7381_cov_6.200655"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(398..955)
                     /gene="def"
                     /locus_tag="PKL42_07370"
     CDS             complement(398..955)
                     /gene="def"
                     /locus_tag="PKL42_07370"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNU66932.1"
                     /translation="MAVKPVLRMGEPCLLQQAQPVTQFDSPELQTLIHDLEDTMKFMN
                     GAGIAAPQIGVSLRVVIFGQKPTQDEGEQKNPRYPDADVVPYTVLINPVITPVGHAMA
                     TDWEGCLSVPGMRGLVPRFERIHYKGVDQYGNTIDRLVSGFHARVVQHECDHLDGVLY
                     PMRIPDLKDFGYTSVFFPNQEMQDE"
     gene            complement(1220..1990)
                     /locus_tag="PKL42_07375"
     CDS             complement(1220..1990)
                     /locus_tag="PKL42_07375"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HNU66933.1"
                     /translation="MLAIIGGTGLTQLDNLEITRRQIVRTPYGEPSQPLLFGEIEGRE
                     VIFLARHGNGHTIPPHQVNYRANISALHLQGVTEIAAVATVGGIHADLASGSIVMPHQ
                     IIDYTHGRKSTFFDGIDLPVKHIDFTDPYCAIMRSNWQQAAHSIDETIINHGVYAATQ
                     GPRLETAAEINRLERDGATIVGMTGMPEAALARELGISYAAICPVANQAAGRGKSLHG
                     IQYEDVIENLNQTMVRVRNIIGALVKLNGLCKINTQKK"
     gene            2128..3027
                     /gene="hpnC"
                     /locus_tag="PKL42_07380"
     CDS             2128..3027
                     /gene="hpnC"
                     /locus_tag="PKL42_07380"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HNU66934.1"
                     /translation="MQNQQQSSTSQHHITTQSIALANQHYENFPVASVFLPKDLRAPI
                     GLIYSFARQADDFADEGHLSTDERLALLDGFRDELDLLQAYIKPKTPFFLTLGEMIKA
                     RKLPYAPFFDLLDAFRQDVIKTRYQHYGEVLDYCTRSANPVGRLMLHLYGQSNENNIA
                     LSDHICSALQMINFLQDIAIDFNKNDGKQRIYMCQDELAQFGITEQDLYTWVNHQAPI
                     NAQWQAFMQFNLNRVQALMQAGKPLGRILTGRIGFEMRMIIAGGERIIHKINVVKGDI
                     FNQRPRLHGGDWLVIFLKALFRI"
     gene            3045..3884
                     /gene="hpnD"
                     /locus_tag="PKL42_07385"
     CDS             3045..3884
                     /gene="hpnD"
                     /locus_tag="PKL42_07385"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HNU66935.1"
                     /translation="MTPQQYCQEQAAKSGSSFYSSFMFLPKPKREAITALYAFCRAVD
                     DVVDEVSDLQVAQAKLNWWRDEIKNLYAHQPQHPITKALLAPIQTYQLDAEHFAEIID
                     GMEMDLNFNRYADFKQLQLYCYRVASVVGLLSAQIFGFNNRKTLKYAHDLGMAFQFTN
                     IIRDVGEDARRNRIYLPLDELAKFKVTEEDILNSHETESVKQLLDFQIERAESYYDRA
                     LNALPIDDRKSQRVGLMMAAIYRTLLREIKKDGAEKVLNSRTSLGTLRKLFLALSVWL
                     KTR"
     gene            3913..5238
                     /gene="hpnE"
                     /locus_tag="PKL42_07390"
     CDS             3913..5238
                     /gene="hpnE"
                     /locus_tag="PKL42_07390"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HNU66936.1"
                     /translation="MSKTHVAIIGGGCAGLSAAATLVERGYAVTLFEASPHLGGRART
                     VLVENNSLMYVLDNGQHILLGAYRETLNLLNKVGVDEAEAFLRVPLKINLHSASSAPD
                     FLLKSESLLPAPLNLLFGLFGCEGLSFTERAAAIKLMAQLKMNGYQIATDTALEAFLL
                     SKNQHARLIEVLWEPLCLAALNTPIAVASTRIFLNVLRDSFTGNKKNSDFLIPKLDLS
                     KIIANPLSQYIQAKGGTIKLDSRVRKLKMENDGQYTISTGSEHLAFSHVIIATPPSRL
                     DNLLTAFPKLQNTLVVTESFGYQPIFTIYLQYPADTKLPNIMTGFTKNISQWAFDKGQ
                     LCGQNGLVSVVVSASGVHQQLSHEELALKVANELHQVFPHLPKPLWNKVIAEKRATFS
                     CVPNLARPSNKTVQPNLFLAGDYTYADYPSTIEGAIRSGIACANLIASG"
     gene            complement(5273..7102)
                     /locus_tag="PKL42_07395"
     CDS             complement(5273..7102)
                     /locus_tag="PKL42_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNU66937.1"
                     /translation="MWILHALPALNPWIESFVDATLLSIFVAVLMYIFVFRNMKKTAA
                     DKFKAEEQLRLVSLAFEMQEAIMITDANANILRVNRAFEKITGYSEQEVIGKNPSILS
                     SGLHDHAFYAKLWSDLLNHGIWKGEMWDRGKNGEIYSKIATISAVKNDKNETVQYIAA
                     FTDNTAHKKAENEIYRLAFYDALTGLPNRRLLLDRLSVALSASTRNQQYGALIFLDLD
                     NFKTLNDTLGHYYGDMLLVEVAKRLKMSVREMDTVARLGGDEFVVLLEDLDADLQEAS
                     RKVAQIAEKLRVKIATPYQLNENIRHSSPSIGVCMFYGNQVSVDDLLKYADMAMYQAK
                     NAGRNRVRFFDPHMQQVVETRALMEADLRSAITDKQLSLYYQVQLDQTMQPVGAEALL
                     RWQHPTRGFVSPVDFIQIAEESSLILDIGDWVLETACQQLAHWGQFEKTRHLVLAINI
                     SAQQFMQTDFVEHVAASVKANQINPACLKLELTESVALGGLDIVVTKMLALKHGIGVT
                     LSLDDFGTGYSSLSYLKKLPFNQIKIDRSFVRDMLTDASDAMMVKNIIDMTHNFGMQV
                     IAEGVETEQQLALLREYGCLSYQGYLFSKPLPAEDFEALINNN"
BASE COUNT         2096 a         1641 c         1552 g         2092 t
ORIGIN      
        1 agttaccgtc aatttataga aactactcta atagacgatg caatattaac gacgaacttg
       61 tcaatgcgca attattattt acattgaaat tacatttatt tacaaactgt tagcatatct
      121 acaacgcgga aattatcgtt aatttttgta ctgaacatca ttcaatcgac acccgattcc
      181 aacaccaaaa tttaaattaa attcattgtt gtttggtatt gctaaattgc actattttta
      241 agtgctactt aaatgccatt gacgttgaat tattcactga gaaaaaataa atgctctgcg
      301 agccttataa ataaaggctc gcagagcatc aaaaaaggtt ggaggattaa attgcgataa
      361 atacgtattt ttgattgctt ataaaagtgc agtttttcta ctcgtcttgc atctcttgat
      421 tagggaagaa aacgctggta tagccaaaat cttttaagtc gggaatgcgc attgggtaca
      481 aaacgccatc aaggtggtcg cactcatgtt gcactacgcg cgcgtgaaag ccgctgacta
      541 agcggtcgat ggtgttgcca tactggtcaa cacctttgta atgaatacgc tcaaaacgcg
      601 ggactaagcc acgcataccc ggcacggata ggcagccttc ccaatcggtc gccattgcat
      661 gacctacggg ggtaatcaca ggattgatca gcacggtgta gggtacaaca tcggcatctg
      721 gataccgtgg gttcttttgc tcaccctcat cttgcgtcgg tttttggcca aaaatcacta
      781 cgcgtaaact cacgccgatt tgtggggcag cgatgcctgc gccattcatg aatttcatgg
      841 tgtcttctaa atcatgaatg agtgtttgca attcaggaga atcaaactgc gtgacaggtt
      901 gtgcttgttg caataaacat ggttcaccca tgcgaagtac tggtttaacg gccatattct
      961 aaatttactt tctgagcctt ttccacaagg cacattttaa ctaaaaaaat tacaatctcg
     1021 tcattcccgc gtaggcggga atctaggctt ggcaaatggt taaatggtta aaggatttaa
     1081 ttacaattag taatcttgaa aattgactgg attcccgcct acgcgggaat gacggattta
     1141 gacaattttc agaaaatagt tcaacttatt ttaagtggta taaaggcctc tttatattga
     1201 ttgatacgca agcctgccac tattttttct gcgtattgat tttgcaaagc ccattgagtt
     1261 ttaccagtgc accgataatg ttgcgcacgc gtaccatggt ttggttaagg ttctcaatca
     1321 cgtcttcgta ttgtatgcca tgcagacttt taccccgccc tgccgcttgg ttggccaccg
     1381 ggcaaatggc cgcataacta atccctaatt cacgggctaa agcggcttcg ggcatccctg
     1441 tcatgccgac aatcgttgcg ccatcgcgct ctaggcgatt aatttcagca gccgtttcta
     1501 aacgtgggcc ttgcgtggcc gcgtatacgc catgattaat aatggtttca tcaatactgt
     1561 gggcggcctg ctgccaatta ctgcgcatga tagcgcaata agggtcggta aaatcaatgt
     1621 gtttcaccgg taaatcaatg ccatcaaaaa aagtgctttt acggccatgt gtgtaatcaa
     1681 tgatctgatg cggcatcaca attgaaccag aggctaaatc agcgtgaatg ccaccgaccg
     1741 tggccactgc tgcaatttct gtcacccctt gtaaatgcag cgcagaaata ttggcgcgat
     1801 aatttacctg atgcggtgga atggtatggc cattgccgtg cctagctaaa aagatgactt
     1861 cacgcccttc tatttcgcca aacagaagcg gttgagaggg ttcaccatag ggtgtacgca
     1921 caatctggcg gcgcgtaatt tccaagttat ccagttgtgt aaggccagtg cccccaatga
     1981 ttgccagcat acggttctct ttacgaatgt gatttaagcc tgattttagc aaagcttgct
     2041 caataattga cataaaaatt cgttttttat cgcgcttggc tacatttcat gaaaaaaaat
     2101 tcgcataatt tatggcatac tcacagcatg cagaatcagc aacaatcaag tacaagccag
     2161 catcacatta ccacgcaaag cattgcgcta gccaatcaac attacgaaaa ctttcccgtt
     2221 gcgtcggttt ttttgcccaa agatttacgc gcgccaattg gcttgattta cagttttgcg
     2281 cggcaggccg atgattttgc cgatgaaggt catttaagta cagatgaacg cctggcttta
     2341 ctggatggct ttcgcgatga acttgattta ctgcaagcct atatcaagcc aaaaacgcca
     2401 ttctttttaa ccttgggtga aatgattaag gcaaggaaat taccctatgc gccttttttt
     2461 gatttattag atgcgtttcg tcaggacgtt atcaaaacgc gttaccaaca ttatggcgag
     2521 gtgttagatt actgcacgcg ctctgccaac cctgtcggtc ggttgatgtt gcatctttac
     2581 gggcaatcaa acgaaaacaa catcgccctt tcagaccata tttgcagcgc gctacaaatg
     2641 attaattttt tgcaggatat tgcgattgat tttaataaaa atgatggcaa acaacgcatt
     2701 tacatgtgcc aagatgagtt ggcacaattc ggcattacag aacaagactt atacacgtgg
     2761 gtgaatcacc aagcgccaat taacgcgcaa tggcaagcgt ttatgcaatt taatttaaac
     2821 cgcgtgcaag ctttaatgca agcaggcaaa ccattgggca gaattttaac aggcagaatt
     2881 ggctttgaaa tgcggatgat tattgccggt ggtgagcgta tcattcataa aatcaatgtg
     2941 gtaaaaggtg atatatttaa ccagcgccct cgcctgcacg gtggcgattg gcttgtgata
     3001 tttctcaaag cactttttag aatttaatag gcacgacgtt actcatgaca ccccagcaat
     3061 actgccaaga gcaagctgct aaaagcggct caagctttta ttctagtttt atgtttttac
     3121 ccaagcctaa gcgcgaggcc attacggcac tgtatgcttt ttgccgcgcg gtagatgatg
     3181 ttgtggacga agtgagtgac ttgcaggtgg cgcaggctaa attgaactgg tggcgcgatg
     3241 aaattaaaaa cctatatgcg caccagccac aacacccgat tacaaaagca ttgctagcgc
     3301 ccattcaaac ctatcaactt gatgccgagc attttgcgga aatcatcgat gggatggaaa
     3361 tggacttgaa ttttaatcgc tatgcagact ttaagcagtt gcaactgtat tgttaccgcg
     3421 tggccagcgt ggtgggtttg ctttctgcac agatatttgg ctttaacaat cgaaaaacac
     3481 tcaaatatgc gcatgatctg ggcatggctt ttcagtttac caatatcatc cgtgatgtag
     3541 gtgaagatgc gcgccgtaac agaatttacc tgcctttaga tgaactggct aaatttaagg
     3601 tcacagaaga agatattctt aacagccatg aaacagaatc ggtaaaacag ttgctggatt
     3661 ttcagattga acgggcagaa agctactatg accgcgcgct gaatgcactc cctatagacg
     3721 accgaaaaag ccaacgtgtt ggcctcatga tggcggccat ttaccgcaca ttgttgcgag
     3781 aaattaaaaa agacggcgcc gaaaaagtat tgaactcgcg cacctctctt ggtacattac
     3841 gaaaactttt tttggcattg tcagtatggt taaaaacacg ttaatgatta tgatacgcaa
     3901 ggtcatgacc ccatgagcaa aacacatgtt gccattattg gcggcggatg cgcagggctt
     3961 agtgcggcgg caacgctcgt tgagcgcggg tatgctgtaa cattgtttga agccagcccg
     4021 catttgggtg ggcgcgcacg cacggtgtta gttgaaaata acagcctgat gtacgtgctg
     4081 gataacggcc agcatatttt attgggcgcg taccgtgaaa cacttaattt actcaataaa
     4141 gtgggggttg atgaagccga agcttttttg cgcgtaccct taaaaattaa cttgcattcc
     4201 gcttcaagtg cgcctgattt tttattaaag tctgagtcgc tgttacctgc tccgctcaat
     4261 ttattatttg ggctatttgg ttgcgaaggt ttaagtttta cagagcgtgc cgctgccatt
     4321 aaattaatgg cgcaacttaa aatgaatggc taccaaattg ccactgacac cgcgttagag
     4381 gcgtttttgc tttcaaaaaa tcaacatgca aggttaatag aagtgctttg ggaaccgctt
     4441 tgcctggcgg cacttaacac gccgattgcc gtggcaagta cgcgcatctt tctaaatgtg
     4501 ttgcgtgaca gctttaccgg caataagaaa aatagcgact ttttaattcc aaaattagat
     4561 ttatcaaaaa ttattgctaa cccgctttcg caatacattc aggctaaagg cggcactatc
     4621 aagctcgata gccgtgttcg caaattaaaa atggaaaacg atggtcaata tacaatttca
     4681 actggaagcg aacacctcgc gtttagccat gtcatcattg caacgccgcc atcacgacta
     4741 gataacttgc taaccgcttt tccaaaatta caaaacacct tggttgtgac ggagtctttt
     4801 ggctaccagc ctattttcac aatttatttg caatatccgg ctgatactaa attacccaac
     4861 atcatgacag gatttacaaa aaacattagc caatgggcct ttgataaagg ccagttgtgt
     4921 ggccaaaatg gcttagtgtc ggttgttgtg agtgccagcg gcgttcatca acaacttagt
     4981 catgaagaac tggcgctaaa agtagccaac gaacttcacc aggtatttcc tcacttacca
     5041 aaaccgttat ggaataaggt gattgctgaa aagcgcgcta ctttttcatg tgttccaaac
     5101 ttggctagac cctcgaacaa aacggttcag ccaaacttgt ttttggcggg cgattacact
     5161 tacgcagatt acccatcgac catagaaggt gcaattagaa gtggcattgc ttgtgccaac
     5221 ttaattgcaa gcggttaatt gaaggtgatt gcgtgttgct taaaaggcca acttaattgt
     5281 tattgataag cgcttcaaaa tcttcagcag gtaaaggctt gctgaacaaa taaccttggt
     5341 acgacaagca gccgtactcg cgcaacaagg ccagttgctg ttctgtttca accccttctg
     5401 caatcacttg cataccaaag ttgtgcgtca tatcaataat atttttcacc atcatggcat
     5461 cactggcatc cgttaacata tcacgcacaa aactgcgatc aattttaatt tgattaaacg
     5521 gcaatttttt caaataagac agtgaagagt aacccgtgcc aaagtcatcc agtgacaaag
     5581 ttacgccaat gccatgcttt aatgccagca tttttgttac cacaatatcc aaaccaccta
     5641 acgcgacgct ttcagttaac tccagtttta ggcatgccgg gttgatttgg tttgctttaa
     5701 ccgatgctgc cacatgttca acaaaatctg tttgcataaa ttgttgcgcg ctgatgttga
     5761 ttgccaacac taaatgtcgc gttttttcaa actggcccca gtgcgccaat tgctgacaag
     5821 ccgtctccag cacccaatcg ccaatatcta atattaaaga actctcttct gcaatctgaa
     5881 taaaatccac tggagacaca aaaccacggg ttgggtgttg ccagcgcaaa agtgcctctg
     5941 cgcctaccgg ctgcattgtt tggtcaagct gcacttggta ataaaggcta agttgtttat
     6001 cagtgattgc actgcgcaaa tcggcttcca tcaaagcccg cgtttcaacg acttgctgca
     6061 tatgcgggtc aaaaaatcgt acgcggtttc tacctgcgtt tttagcttga tacatcgcca
     6121 tatcggcgta ttttaacaag tcatccacgc ttacttgatt gccataaaac atgcacacgc
     6181 caatgcttgg gctactgtga cggatatttt cattgagctg gtaaggcgtt gcaattttta
     6241 cgcgcaattt ctctgcaatc tgcgcaactt ttcggctggc ttcttgcaaa tccgcatcta
     6301 aatcttcaag caacaccaca aattcatcac cccctaaacg ggcaacggtg tccatttcac
     6361 gcacactcat tttgagtcgc ttggccacct caaccagcag catgtcgcca tagtagtggc
     6421 caagggtgtc attgagcgtt ttaaaattat ctaaatccaa aaagattaac gcaccatatt
     6481 gctgatttcg cgtagaggct gagagcgcaa cacttaagcg atcgagcaac aatcggcggt
     6541 ttggcaagcc tgttaatgca tcgtagaagg cgaggcggta aatctcattt tcagcttttt
     6601 tatgggctgt gttatcggta aaagctgcga tatactgcac ggtttcattt ttatcgttct
     6661 tgacggcaga aatcgtggca atcttagaat atatttcacc atttttgcct ctatcccaca
     6721 tttccccttt ccaaatgcca tgattcagca agtcactcca taactttgca taaaacgcat
     6781 ggtcgtgcag accagagctc aaaatgcttg ggtttttgcc gatgacttct tgctcggaat
     6841 atcctgtgat tttttcaaaa gcacgattca cacgcaatat gttggcattg gcatcggtaa
     6901 tcataatggc ttcttgcatt tcaaacgcta acgaaacaag gcgcaactgc tcttctgctt
     6961 taaatttatc tgcagcggtt tttttcatat tacggaacac aaaaatatac atcagcactg
     7021 caacaaaaat agaaagcaaa gttgcatcaa cgaatgactc tatccatggg tttaatgcgg
     7081 gcaaggcatg caatatccac ataatgatca gctcgcccac aaaaatgcag ccagtcagca
     7141 ctaaccatta atattgattt gttttagaaa tattcattca tgcaccagaa atcggtaatt
     7201 aaattgataa tacgctgcta tgtactaagc tgattaccac aataaaacca ttaattaggc
     7261 gtaattcatt agatgtaaat ttttgtatta ccctttatca aacagctcaa tttaactacg
     7321 caaaaactcc cgagcaaaaa acattgccct gcgagccttt atttacttgg cttgcagggc
     7381 a
//
LOCUS       DAOQSI010000107         7359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_9792_length_7359_cov_5.392662, whole genome shotgun sequence.
ACCESSION   DAOQSI010000107 DAOQSI010000000
VERSION     DAOQSI010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 7359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7359
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9792_length_7359_cov_5.392662"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2398)
                     /locus_tag="PKL42_07400"
     CDS             complement(<1..2398)
                     /locus_tag="PKL42_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
                     /protein_id="HNU66938.1"
                     /translation="MKINMPVTQNQVELKDNSQIVSKTDLKGRITFVNREFIDVSGFT
                     EAELIGQSHNIVRHPDMPPEAFEDLWSTVKNGKPWIGIVKNRCKNGDYYWVEAVVAPV
                     VENGQPAGFISVRKKASRQQIEGAMRFHRKIKEGQSWAATQLDKLRAFKRNFSLRARI
                     ISIFLTVVLTTGFVGFIGLKGIEDTNQAVHSLYENRVVAIEQLKRVADMYAVNIVDTS
                     HKVRNGNMTWAEGEANVEVALKEIDTQWKAYHAKTLEAEEQKMAADIESLFANANASV
                     ATLQAILRAHDDKRLAEFTAKELYPAIDPISDRVNALTNLQTKISGQVFAEAEEDFQL
                     HFKGGMLFLVLGILITGYLGRLLFQAIMPRVHDAVRYLLLSAQGVDHESVARTGHRDE
                     LTDVMDAYRALKTRLDFDHAETLAGVNRIKSALENSSMAVTVSNEHNALIYMNKAAKT
                     LFSKMSRGIAVRHPGFTVEKMMGTKVGQYLENETDRENFAQPLSGSKQISTVNAGLHL
                     DMLLNPVYDKETGNYLGRMTQWVDRTAEVFAQQKVAELIDQTVAGDLTKRIDATTLPE
                     GFVRDISKGINQLLEAVINPLNMAADYVDNLSKGVIPAEITSEYQGDFNIIKNNLNAC
                     GQAIKALVADGNLLAEAAEAGVLTTRADATQHLGEFRKVVEGLNATLDAIVTPLNMAA
                     ENLASIARGEIPAKITAQYQGDFNNIKDNLNTCIESINALVNDVQMLATAAHDGRVSV
                     RADVSAHEGDFRKIVEGVNETLEMIVGPIGTVKVAVETINTAAKEISQGNADLSRRTE
                     E"
     gene            complement(2597..3928)
                     /locus_tag="PKL42_07405"
     CDS             complement(2597..3928)
                     /locus_tag="PKL42_07405"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HNU66939.1"
                     /translation="MHTNKHKVVVAAAAGINLVIGVLYAWSIFKEAIVASINTGGPNA
                     FHWNLASINDPYALCCLIFAIVMVPAGRMQDSIGPKKVALLGGLLGAIGFVLIANSVN
                     YWVWMFGFGGLVGAGIGFAYASTTPAALKWFAPNKSGLVTGIVVSGFALASVYVAPLA
                     SHLVIVYGLNKAMLFFALQFFLLISLFSFLLHTPPHGYMPPGFEERRVARNDRKRDAM
                     SHLDEDVLSPLHVLKESRFWVLWVLLFIGAGAGLMVIGNLKPLAKLSMGELAYLAIVI
                     LAVGDAAGRILTGALSNRFGRRNVLSAAFLLQMLLMFGAFFLSQSGNAVWIVLAATFI
                     GMSYGANLVLFPNYVKDFWGMRHFGAIYGMLFTAWGVGGFVMIKVAENLTVNSGSSFQ
                     SFMVAGCLMLVAFMLTFRVDNRKDIERAAMRRAKKQNLVQQKNLSEITPVA"
     gene            complement(4074..7067)
                     /locus_tag="PKL42_07410"
     CDS             complement(4074..7067)
                     /locus_tag="PKL42_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNU66940.1"
                     /translation="MNFIKSMTVAQKLFAIIAIAVTVATVQAGIGLFELSKSRDSLKT
                     VYEDRMVPLEQLDSIAINMLKNRTHINAALNETHTVIGPDKKPILALDTQNALKEAQY
                     IESKIEEISKTWAVYMATNLTPEEKLLAEKFSDSRGEFVTKILKPAIVKLQSGNYQEL
                     AQINAKTQDLFMLAEKDLLALKTLQLDTAAKEYQSAASSFENIRLLTFAGLAFAVILM
                     MVVGYAISRSITKALGGEPGAMYDAANRIAAGNLDFKINLAANDSTSAMAAMSNMQSN
                     IKQLVADASMLSTAAKEGRLATRADANRHLGDYRKVVAGVNETLDAVIGPLNVAANYV
                     DRISKGDIPAKITDNYNGDFNAIKQNLNTCIDAVNNMVTEAVSLEKSAIDGQLNTRAD
                     ANKYQGDFRKVIEGFNKSLDAIVTPLTMAAGYVEKLAKGDMPAKITDSYNGDFNVIKN
                     NLNTCIDAISLLVFDANKLAQAAQDNKLDERADAKKHFGDYRKVIEGVNGTLDTVMAK
                     NTTDAAQKAKEAQALQNAVEETQEVIESAKTGDLSNRVSLAGKTGAIASLCDGVNALM
                     DKMTEVVVQVREASETINTAAGEISSGNNDLSSRTEQQASSLEETAASMEELASTVKN
                     NAENAKQANQLAASASSVAIKGGEVVGQVVATMSSINESAKKIEDIISVIDGIAFQTN
                     ILALNAAVEAARAGEQGRGFAVVAGEVRNLAQRSASAAKEIKELITDSVSKTAEGTVQ
                     VENAGKTMQEIVSSVQRVTDIMGEIAAASSEQSSGIDQVNNAITSMDEVTQQNAALVE
                     EAAAAAESLVEQAVTLMDTVNEFKLNGGNQVTNNRRASNSPMRRPNATVSKAAGLAKP
                     ETKFSFADATQAHIKWKNRLVNYVSGNSDEHLEVEKVCRDDQCPLGGWIHGEARKHAH
                     LHEYKELKTAHAEFHQSVGDIVQHVHDNKQAEARKLLGADFSRTSKRTIAAIQEIESV
                     VNANSANHLKTVRKNGTDDGEWEAF"
     gene            complement(7136..>7359)
                     /locus_tag="PKL42_07415"
     CDS             complement(7136..>7359)
                     /locus_tag="PKL42_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66941.1"
                     /translation="TLMDTVNEFKLNGGNQVTNNRRASNSPMRNAAPSRPAATRSVAS
                     RPVAKPVSRPAPVKAIAKTGTDDGDWEEF"
BASE COUNT         1949 a         1756 c         1375 g         2279 t
ORIGIN      
        1 gttcttcggt gcgacggctt aggtctgcgt tgccttgcga gatttctttg gcagccgtgt
       61 tgatggtttc taccgcaact ttgacggtgc cgatggggcc gacgatcatt tcgagggttt
      121 cgttcacccc ttctacaatt ttacggaagt caccttcatg agcactgacg tcagcacgaa
      181 cagacacacg accatcgtgc gcagcagtgg ctagcatttg aacatcattt accaaggcat
      241 taatcgattc aatacaagtg ttcaaattat ctttaatgtt gttaaagtca ccttgatatt
      301 gcgcagtaat tttcgctgga atttcgccac gggcaatgct ggctaagttt tcagccgcca
      361 tattaagtgg cgtcacaata gcatccagtg ttgcattcaa gccttcaaca actttgcgga
      421 attcgcctaa atgttgcgtg gcatctgcgc gggttgtgag cacgcctgct tcagctgctt
      481 ctgctagtaa attaccatcc gctactaatg cttttatcgc ctgaccacaa gcatttagat
      541 tgtttttgat gatattaaaa tcaccttgat attcactggt aatctcagct ggaatgacac
      601 cttttgataa attatcaaca taatcagccg ccatattgag tgggttaatc acagcttcaa
      661 gcaactgatt gatgcccttg ctaatatctc gcacaaaacc ctctggcaac gttgtcgcat
      721 caatacgctt ggttaagtcc cctgctacag tttgatcaat caattctgct actttttgct
      781 gcgcaaacac ttctgccgtg cggtcaaccc attgcgtcat gcggcctaaa tagttaccgg
      841 tttctttatc ataaacgggg ttgagtagca tgtctaaatg cagcccagca ttgaccgtgc
      901 taatttgttt actgcctgaa agcggttgtg caaagttttc acggtcagtc tcattttcaa
      961 gatattgccc aaccttggtg cccatcattt tttcaacggt aaagccaggg tgacgaactg
     1021 caataccacg actcatttta ctgaataacg tctttgctgc tttattcatg taaatcaatg
     1081 cattatgttc gtttgaaact gtcactgcca tggatgagtt ttccaaggct gatttaatac
     1141 gatttacccc tgccagcgtt tcagcatggt caaagtcaag gcgtgttttt aatgcacggt
     1201 aggcatccat cacatccgtt agctcatcac gatggcctgt acgtgcaaca gattcatggt
     1261 ctacaccctg cgcacttaat agcaagtaac gcacggcatc atgcacacgc ggcataatcg
     1321 cctgaaataa taatctgcct aaataccctg ttatcaaaat ccctaacact aaaaacagca
     1381 ttccaccttt aaagtgcaac tgaaaatctt cttctgcttc ggcaaatact tggcctgaaa
     1441 tttttgtttg aagattcgtc agcgcattta cgcggtcaga aattggatca atcgcaggat
     1501 agagttcttt tgcagtaaat tcagcaaggc gtttatcatc gtgcgcacgt aaaatagctt
     1561 gtaacgtggc aacacttgcg ttggcattgg caaataacga ttcaatatct gctgccattt
     1621 tttgctcttc agcctccaat gttttagcat ggtaggcttt ccactgcgta tcaatttctt
     1681 ttagtgcaac ttccacattg gcttcacctt cagcccacgt catattgcca tttcgcacct
     1741 tgtgtgaggt atccacaata ttcacggcat acatatctgc cacacgtttg agttgctcaa
     1801 tcgcaaccac gcggttttca taaagtgaat gcactgcttg attggtatct tcaatacctt
     1861 ttagaccaat aaatcctaca aaacctgtcg ttaacaccac ggttaaaaaa attgaaataa
     1921 tgcgtgctct taaactaaag tttcgtttaa atgcgcgtaa cttatctaac tgcgttgccg
     1981 cccagctttg gccttctttt attttacggt gaaaacgcat cgcgccttca atttgctggc
     2041 gtgaagcttt ttttcgtact gaaataaagc ctgctggttg gccattttca accactggcg
     2101 caactactgc ctcaacccaa taataatcac catttttgca acggttttta acaatgccaa
     2161 tccaaggttt gccgttttta acggtagacc ataaatcttc aaacgcttca ggtggcatgt
     2221 ctgggtggcg cacaatatta tggctttgcc ctatgagttc ggcttctgta aaaccactca
     2281 catcaataaa ctctctgttt acaaaggtaa tccggccttt taagtcggtc tttgaaacaa
     2341 tttgtgagtt atcttttaac tcaacctgat tttgcgttac gggcatatta attttcattt
     2401 ttatttcctt ttttggtgtt acccactttt aacttttaca cttttggtgt ttaagtcatt
     2461 tttctatttc aatattgaga atctcggcgc gtcattcacg cgttatttca caaagctgtt
     2521 caacttttat tacggcaatt ttttacaaat atttactttt tttaatagaa attttggtca
     2581 attttttatc atcaactcaa gcaactggcg ttatctcaga aagatttttc tgttgcacca
     2641 aattctgctt tttcgcccta cgcatggctg cacgttcaat gtctttgcga ttatccacac
     2701 gaaaagtcag cataaaagcc actagcatta aacaccctgc caccataaac gattgaaaac
     2761 tagaaccaga attcaccgtc aagttttcag ccactttaat catgacaaag ccaccaacgc
     2821 cccaagctgt aaacaacatg ccgtagattg caccaaagtg cctcatgccc cagaagtctt
     2881 ttacgtaatt tggaaaaagc actaaatttg caccgtagct catcccaata aaagttgctg
     2941 ccagaacaat ccataccgcg ttgcccgatt gtgatagaaa aaatgcgcca aacatcagta
     3001 acatttgcaa taaaaaggct gcacttaaaa cgtttcggcg accaaagcgg ttagaaagtg
     3061 cgcctgttaa aatgcgccct gctgcatcgc caaccgctaa aattacaatc gcgagatacg
     3121 ccaattcacc catactcaat ttggcaagtg gttttaaatt accaatcacc ataaggccag
     3181 cacccgcgcc aataaaaagc aacacccata acacccaaaa ccttgattct ttcaacacat
     3241 gcaaagggga aagcacgtct tcatcaagat gcgacatcgc atcacgtttt ctatcgttac
     3301 gagcaacgcg tctttcttca aagcctggtg gcatatagcc atgtggcggc gtatgtaata
     3361 aaaatgaaaa cagtgaaatc agtaagaaaa attgcaacgc aaagaaaagc atcgctttat
     3421 ttaagccata cacaatcaca agatgactcg cgagcggtgc aacatatacc gaagccagcg
     3481 cgaagcctga aaccacaatg cctgtgacca agccgctttt attcggtgca aaccatttta
     3541 atgctgcagg tgttgtagag gcataagcaa aaccaatacc agcgccaact aaaccaccaa
     3601 aaccaaacat ccaaacccaa taattaactg aatttgcaat gagcacaaac ccaatagcac
     3661 cgagcaagcc gcctaataaa gcaacttttt ttggcccaat agaatcttgc atacgccctg
     3721 ccggcaccat cacaattgca aaaattaggc aacataaggc atacggatca ttgattgagg
     3781 ctagattcca atgaaaagca ttggggccgc cagtgtttat tgaagcaaca atggcttctt
     3841 taaaaatgct ccacgcatac agcacaccaa tcaccaagtt aatacctgcg gcggcggcca
     3901 caacgacttt atgtttgttg gtgtgcattt tttattcaaa catcatcaag taaatataaa
     3961 ttagaactca tagccaatca ttgaagatga ccatattttc attgcttcta ccataagctt
     4021 tgcccattct gaaaagtgtt tgctcatctt caccccccta cattatcaat aaactaaaac
     4081 gcttcccatt caccatcatc ggtaccattt tttctaaccg ttttaagatg gtttgcacta
     4141 tttgcgttta caacagattc aatctcttga atagccgcaa tggtacgttt agaggttctt
     4201 gaaaaatcag cccctaataa ttttcgcgct tcggcttgtt tgttatcgtg cacgtgctgc
     4261 acaatgtcac caacactttg gtgaaattcg gcatgggctg ttttgagttc tttatattca
     4321 tgcaaatgcg catgtttacg tgcttcgcca tgtatccaac cccctagtgg gcattggtcg
     4381 tcacggcata ctttttcaac ttctaaatgc tcatcagagt ttcctgacac atagttcact
     4441 aaacggtttt tccatttaat gtgcgcttgc gtggcatctg caaatgaaaa ttttgtttct
     4501 ggttttgcaa ggccagccgc tttactcacc gttgcatttg gtctacgcat tgggctgtta
     4561 cttgcgcgac gattatttgt cacttggttg ccaccattta acttaaactc gttcacggta
     4621 tccattaatg tcaccgcttg ctcaaccagt gattcagcag ccgcagccgc ttcttctact
     4681 agtgcggcat tttgttgcgt cacttcatcc atgctggtaa tcgcgttatt tacttggtca
     4741 atacctgagc tttgctcgct tgatgctgca gcaatttcac ccatgatgtc agtcacacgt
     4801 tgcactgaac tcacaatttc ttgcatggtt ttaccagcgt tttcaacttg tacggtgcct
     4861 tcagcggttt tgctgactga atctgtaatg agttctttaa tctctttagc tgcactggct
     4921 gagcgttggg ctaggttacg cacttcaccc gctaccaccg caaaaccgcg accttgctca
     4981 ccggcacggg ctgcttcaac ggcagcgttt agcgcaagaa tattagtttg gaaggcaatg
     5041 ccatcaatga ctgaaataat gtcttcaatc tttttagcgc tttcgttgat ggagctcata
     5101 gtagcaacca cttgaccaac gacctcacca ccttttatcg ccacgcttga ggcactggcc
     5161 gcaagttgat tggcttgctt ggcgttttct gcattgtttt taacagttga ggcgagttct
     5221 tccatgctcg ctgctgtttc ttcaaggctt gaggcttgtt gttctgtgcg gctagatagg
     5281 tcgttgttcc ctgaagaaat ctccccagcg gcagtattga tggtttcact tgcttcacgt
     5341 acttgcacta ccacttcagt cattttatcc atcagggcat ttactccgtc acaaagggaa
     5401 gcaatcgcac ctgttttacc agctaatgaa acacggttgg ataagtcgcc tgttttagct
     5461 gattcaatca cttcttgcgt ttcttcaact gcattttgaa gtgcttgtgc ttctttagct
     5521 ttttgtgcgg catcagtagt atttttcgcc atcactgtat ctagcgtacc atttacaccc
     5581 tcaatgactt tgcggtaatc gccaaagtgt ttttttgcat ctgcacgttc gtctaatttg
     5641 ttatcttgcg ccgcttgtgc aagtttgttt gcatcaaaca ccaataaaga aatcgcatca
     5701 atacaggtat tcaaattatt tttaatcaca ttaaaatcac cgttataact atcggtaatt
     5761 ttggcaggca tatctccttt tgcgagtttt tcaacatagc cagcagccat agtcaatggc
     5821 gtaacaatgg catcaagaga tttattaaat ccttcaatca ctttacggaa atcaccctga
     5881 tatttattgg catctgcacg tgtgttcagt tgcccatcta ttgcagactt ttcaagacta
     5941 acagcctcgg tcaccatatt gttcacagcg tcaatacagg tatttaaatt ctgtttaatc
     6001 gcgttgaaat caccgttata gttatctgta attttggctg gaatatcccc tttagaaatt
     6061 cggtcaacgt aatttgcggc aacatttaat ggcccaatca ctgcatctaa ggtttcattc
     6121 accccagcaa ccactttacg gtagtcgccc agatgtcgat ttgcatctgc acgggtggct
     6181 aatcgcccct ctttggctgc ggttgataac atcgaagcat cggccactaa ctgcttaata
     6241 ttactttgca tattactcat ggcggccatt gcactggtgc tatcattggc ggcaaggtta
     6301 attttaaaat ctagattacc tgccgcaata cggttagctg cgtcatacat tgcaccgggt
     6361 tcgccaccaa gtgcttttgt aattgaacgt gaaatcgcgt aaccaacgac catcatcaaa
     6421 attacagcaa aagctagacc tgcaaaagtg agtaagcgaa tgttctcaaa acttgaagca
     6481 gctgattgat actctttagc agcagtatct aactgcaatg tttttaaagc caataagtct
     6541 ttttcagcca acataaataa atcttgggtt tttgcattga tctgcgctaa ctcttgataa
     6601 ttcccagatt gcaattttac aatggcgggc ttcaatattt ttgttacaaa ttcacctcgg
     6661 ctatctgaaa atttttcagc caaaagcttt tcttctgggg ttagattcgt ggccatataa
     6721 acggcccagg tcttactgat ttcttcaatt ttactttcaa tatactgcgc ttcttttaat
     6781 gcattttgag tatctagtgc caatattggt tttttatctg gcccaatgac agtatgcgtt
     6841 tcatttaatg cagcattgat atgcgtacgg tttttaagca tattgattgc aatactgtcc
     6901 aactgctcaa gtggcaccat tcggtcttca tagaccgttt tcaggctatc tctactttta
     6961 cttaactcaa ataagccaat acctgcctga actgttgcta cagtgacagc aatggcgatg
     7021 attgcgaaca acttttgagc aaccgtcatt gatttaataa aattcatttt tttatcctat
     7081 taatacttta ttgaacagct agaatctatt cagaacaaaa gcccttctca cctgtttaaa
     7141 actcttccca atcaccatca tccgtgccag tttttgcaat ggctttcact ggcgctgggc
     7201 gtgacactgg ctttgctact ggacgacttg cgactgatct tgtggctgct ggtcggctcg
     7261 gtgcagcatt ccgcattggg ctgttgctag cacggcgatt attagtcact tggttgccac
     7321 catttaactt aaactcgttc acggtatcca ttaatgtca
//
LOCUS       DAOQSI010000108         7290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_9940_length_7290_cov_5.437042, whole genome shotgun sequence.
ACCESSION   DAOQSI010000108 DAOQSI010000000
VERSION     DAOQSI010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 7290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7290
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9940_length_7290_cov_5.437042"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /locus_tag="PKL42_07420"
     CDS             complement(<1..1419)
                     /locus_tag="PKL42_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HNU66942.1"
                     /translation="MNQTLNQTNNHLLAPKAQGLYRPSNERDGCGVGFVAHIKGKKSH
                     QIVQQGLALLTNLTHRGATGYDPKLGDGAGLLMQLPDAFMRKEAAKLNITLPPAGQYA
                     VGNVFLPQLAKNRAACEALFTKIIAEENQTLLGWRDVPVDNSNIAQAARDVEPVMRQV
                     FIASTGTDQNPESQNKFERKLFVIRKRIEHAVRALNLQDNAMFYLPSLSSRTIVYKGM
                     LLANEVGVYYKDLSDGTVVSALAMVHQRFSTNTFPAWDLAHPFRMIAHNGEINTIQGN
                     VNWVAARHETMKSSLIGEDLEKLWPLIVDGQSDSACFDNCLELLVAGGYSLPHAMMML
                     IPEAWGAKDAAGKPLMEEERRAFYEYHAALMEPWDGPAAVAFTDGRMVGATLDRNGLR
                     PARYLVTDDDIVMMASEMGVITFPQERIVKKWRLEPGKMLLIDMEQGRIIADAEVKNA
                     LAGAKPYQQWLKKSRYFLCDMPK"
     gene            complement(1934..3286)
                     /gene="gdhA"
                     /locus_tag="PKL42_07425"
     CDS             complement(1934..3286)
                     /gene="gdhA"
                     /locus_tag="PKL42_07425"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HNU66943.1"
                     /translation="MPYVAETIARLKKSAPAEVEFYQAVEEVLTSLRPLLDKNPKYRK
                     HGIIERIVEPERQISFRVSWIDDKGHVQVNKGYRVQYSSAIGPYKGGIRFHPTVYSGI
                     IKFLGFEQIFKNSLTGLAIGGGKGGSDFDPKGKSDNEIMRFCQSFMTELYRHIGATVD
                     VPAGDIGVGGREIGYMYGQYKRLTGSYEGVLTGKKLNWGGSLARTEATGYGAVYFAQN
                     MLEGRGDSLNGKTCVVSGAGNVAIYTIQKLHQLGAKAVTCSDSRGFIYHEKGIDLALL
                     KQLKEVEGASLEKYLETHQDAKYTPVSAYPAGRNAVWSVPCQAAFPSATQNELNEADA
                     KELLKNGCICISEGANMPSTPEAVEAFLKAKICYGPGKAANAGGVATSQLEMAQNGSM
                     QQWTFEEVDAKLKDIMKNIYIRASEAAAEFGEPTNLVLGANIAGFRKVADSMIDQGVV
                     "
     gene            complement(3467..4765)
                     /gene="hemL"
                     /locus_tag="PKL42_07430"
     CDS             complement(3467..4765)
                     /gene="hemL"
                     /locus_tag="PKL42_07430"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HNU66944.1"
                     /translation="MTAPAISQNQTLFEKSQALIPGGVNSPVRAFRSVGGTPIFFKKG
                     LGSKLWDVDGKEYIDYINSWGPMIVGHAHPAVISAVQEAAANSLSFGAPTGLELEMAQ
                     LINKLVPSMEQVRLNSSGTEATMSAIRTARGFTGRDKIVKFEGCYHGHSDGLLVKAGS
                     GLLTFGEPDSGGVPASVAAHTLTLEYNNTQQLKDLFAKSGDEIACVIIEPVVGNMNLI
                     VPHMEFLQTLRELCTQHGAVLIFDEVMTGFRVALGGAQALYNIKPDMTTLGKVIGGGL
                     PVGAFGGRKDIMQCLAPVGKVYQAGTLSGNPVAVTAGLKTLALIQAPDFHAKLSAQTQ
                     KLVDGLKAVAKEAGVIFNAQSVGGMFGLYFCEKCPTSYHEMMQSNKEHFNQFFHSMLD
                     SGIYLGPSAFEAGFVSAAHSDADIATTLNAAKVAFSSIKI"
     gene            complement(4886..5542)
                     /gene="thiE"
                     /locus_tag="PKL42_07435"
     CDS             complement(4886..5542)
                     /gene="thiE"
                     /locus_tag="PKL42_07435"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNU66945.1"
                     /translation="MNSPHKIRGLYAVTLDEPDTALLIQKVTAALQGGVSLLQYRNKA
                     ANHKLKTQQARALLPLCRQYGVPLIINDSIKLCLTLDADGVHLGAEDGDLKEARARLG
                     AGKILGASCYNRIDLATQAQSAGADYVAFGACFGSNTKPNARHAPLSLFKEARQHIHV
                     PMVAIGGITLENAQQVLQAGADAIAVVNALFNAKNITETSHLLSRYFPRENFDALRAL
                     "
     gene            complement(5557..6417)
                     /locus_tag="PKL42_07440"
     CDS             complement(5557..6417)
                     /locus_tag="PKL42_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HNU66946.1"
                     /translation="MSNQSPPSVLTFAGTDPSSGAGLQADILTFASIGCHPLSVVTAI
                     TAQDTIGVNGVLAMDAEWVNDQARAILEDVQVSAFKLGLLGSVENIAVIAEIMADYPD
                     VPLLIDPILTSGRGDDLSNDEMMEAMIELLFPQATLITPNSLEARRLAFAEDSDEVAL
                     TSLDESAARLLAMGSEYVLITGTHERTLEVTNTLYGENNFVKAYKWERLVGSYHGSGC
                     TLTSAIAACMAHGLTLEEAVLEAQEFTWQTLKNGFRPGMGQYIPDRLFWARDEEDAVV
                     TSGDVTTKAH"
     gene            complement(6645..6881)
                     /locus_tag="PKL42_07445"
     CDS             complement(6645..6881)
                     /locus_tag="PKL42_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNU66947.1"
                     /translation="MTADEFREGLATLGWKQTDFAMATGLSKVAVSHWLTGTAPLPLW
                     AQSHLSLLLKLHDLAGELLTPPTKAAKQDKKIND"
BASE COUNT         1972 a         1728 c         1505 g         2085 t
ORIGIN      
        1 ttttggcata tcgcacaaaa agtagcgaga ttttttcagc cattgctggt aaggctttgc
       61 gccggcaagt gcatttttga cttcagcatc ggcaataata cggccttgct ccatgtcaat
      121 cagtaacatt ttgccaggtt ctaaccgcca ttttttaaca attctttctt gcggaaacgt
      181 aatcacgccc atttctgacg ccatcatcac aatgtcatca tcggttacca aataccgtgc
      241 cggacgcaag ccattgcggt ctaaggttgc ccccaccatg cgcccatcgg taaacgctac
      301 ggccgctggg ccatcccatg gctccatgag tgctgcgtgg tattcataaa aggcacgacg
      361 ctcttcttcc atcaacggct tgcccgccgc atcttttgca ccccaagctt caggaatcaa
      421 catcatcatg gcatgtggca agctgtatcc acctgctacc agtaactcta agcaattatc
      481 aaagcaggca gaatcagatt ggccatcgac aatcaatggc cacagctttt ctaaatcttc
      541 accaatgagg ctcgatttca tggtttcatg gcgcgccgcc acccaattga cattgccttg
      601 aatggtgtta atttcaccat tgtgcgcaat catgcggaat ggatgggcta aatcccaagc
      661 ggggaaggtg ttggtagaaa aacgttgatg caccatcgcc aaggcagaaa ccaccgtgcc
      721 atcgcttaaa tctttgtaat aaacgcccac ttcattggcc agcaacatgc ctttgtaaac
      781 aatggtgcgg gatgaaagcg atggcaggta aaacatagcg ttgtcttgca aattaagtgc
      841 gcgcaccgca tgctcaatgc gtttacgaat cacaaataat ttacgctcaa atttattctg
      901 actctctgga ttttggtctg tacccgttga tgcaataaac acctgacgca tcaccggctc
      961 aacatcacgc gcggcttgcg cgatgttgct gttatccacg ggtacatcac gccaacccaa
     1021 taatgtttgg ttttcttctg caataatctt ggtgaataag gcttcacatg ccgcacggtt
     1081 tttggcaagc tgcggtaaaa atacattgcc taccgcatat tggccagcag gcggcaaagt
     1141 aatgttgagt ttagccgcct ctttacgcat aaacgcatct ggcaattgca tgagcaagcc
     1201 tgcgccatcg cccagcttgg ggtcataacc cgtggcacct cgatgcgtta aattggtgag
     1261 cagggctaag ccttgctgca caatttgatg gcttttttta cccttgatgt gcgctacaaa
     1321 acccacgcca cagccgtcgc gctcattact cgggcgatat aagccctgcg cttttggtgc
     1381 taacaaatga ttgtttgtct gatttaatgt ttgattcaat gcttgattca cagcctgctc
     1441 catcaacctt aaccctcatg tttgactaca ctttagccag cttactacca atacaaaata
     1501 cgctggggta aaagtattaa gcaaaaagtg tgcaagctat aatgagttca caaatatttt
     1561 acatatttac tcattaatat ttatataaat cataaggttg taagtttgtt tgacgctttg
     1621 ttacaaatat tttcgcatgg tagtcgataa tatttacgcg ctcaaacacc attgatgcac
     1681 caagatagtg ctggcgtatt tgtatgtgca ctgttttaga actttttagt aattaacgct
     1741 gaaaaatgga cggtgaaaag tagctaacta agcagctaaa taacacgaga aaaaaacgct
     1801 gccctgtaag cctttattta taaggcttac agggcaggtg aaaaggttta ggctctttga
     1861 ctataaaaaa taaattttaa acacaaaaaa ccccgcaact ttcgttgcgg gtttgaccta
     1921 actaaaagct cgcttacaca acgccttggt caatcattga gtctgctacc ttacggaagc
     1981 cagcaatgtt tgcacccaat accaagttgg ttggctcgcc aaattctgcc gccgcttcac
     2041 ttgcacgaat gtagatattt ttcataatgt cttttaattt ggcatccact tcttcaaacg
     2101 tccattgttg catgctgcca ttttgcgcca tttctaactg gctagttgcc acgccacctg
     2161 cgttagccgc tttacctggg ccgtagcaga ttttagcttt tagaaacgct tcaaccgctt
     2221 caggggttga aggcatgttg gcgccttcag aaatacaaat acagccattt ttcaataact
     2281 cttttgcatc ggcttcgttc agctcatttt gcgttgcact tggaaaagcc gcttggcaag
     2341 gcacagacca cactgcattt ctacctgcag ggtaagcgct cacaggggta tattttgcgt
     2401 cttgatgcgt ttcaaggtat ttctctagcg atgcgccttc aacttctttt agttgtttaa
     2461 gcaaggctaa atcaatgcct ttttcgtggt agataaaacc gcgtgaatcg ctacatgtga
     2521 cggctttagc acctaattgg tgcaattttt gaatcgtgta aatcgcaacg ttacccgcac
     2581 cagaaacaac gcaggttttg ccattgagtg aatcaccacg gccttcaagc atgttttgtg
     2641 caaagtaaac agcaccgtaa cctgtggctt cagtacgcgc tagtgagccg ccccagttaa
     2701 gttttttacc agttaacaca ccttcgtagc tgcctgttaa acgcttgtat tggccataca
     2761 tgtagccaat ttcacggcca cccacgccaa tgtcacctgc tggcacatcc acagtagcac
     2821 caatatggcg ataaagctct gtcataaatg attggcagaa acgcataatt tcattgtctg
     2881 atttgccttt tggatcaaag tcgctaccgc ctttgccgcc gccaattgcc aaacctgtta
     2941 atgagttttt gaaaatttgc tcaaagccca agaacttaat aatgcctgag taaacggttg
     3001 ggtgaaaacg aatgccgcct ttataagggc caatcgctga actatattga acgcggtagc
     3061 ctttattgac ttgcacatgg cctttgtcgt caatccaaga aacacggaaa gaaatttgac
     3121 gttctggctc tacaatacgc tcaataatgc cgtgttttct gtattttgga tttttatcta
     3181 acaacgggcg aagtgaagtt aacacttctt caaccgcttg gtaaaattca acttctgctg
     3241 gggctgattt ttttaatctg gcaatggttt cggctacata gggcatgatg tcttctcaaa
     3301 tttagggttt aaatatagtc acaaccgcta aatgtcatga ttattttaca tcacatcatg
     3361 actcaactta ccaaatgcac ttgatgagtg cattattttt agcgggcaga tactttaccc
     3421 caaaatggtg catgttcgca agaattcagc cttaagtttt taatccttaa attttaatgc
     3481 ttgaaaaagc tactttagcc gcgttgagcg ttgtagcaat gtctgcatcg ctatgcgcgg
     3541 ctgaaacaaa acctgcttca aaggccgatg ggcctaaata aatgccgcta tctaacatgc
     3601 tgtggaaaaa ctgattaaag tgctctttat tggattgcat catttcatga taagaagttg
     3661 ggcatttttc gcagaaatac aggccgaaca tgccgcccac actttgtgcg ttaaaaatta
     3721 cgcccgcttc ttttgcaact gcttttaggc catctaccaa tttttgggtt tgcgctgata
     3781 attttgcgtg aaaatctggc gcttgaatca acgctagcgt ttttaaaccc gccgttactg
     3841 caaccgggtt gcccgatagc gtgcctgcct gatacacctt acccactggc gccaagcatt
     3901 gcataatatc ttttcggccg ccaaacgcgc ccacaggcaa gccgccacct atcactttac
     3961 ctaaagtcgt catgtctggt ttgatgttat acagcgcttg tgcgccacct aaagccacac
     4021 ggaagcccgt catcacttca tcaaaaatca gcacggcgcc atgttgggtg caaagctcgc
     4081 gcaaggtttg taaaaactcc atgtgtggca caatgaggtt catattaccc accacaggct
     4141 caataatcac acaggcaatt tcatcgcccg atttggcaaa taaatccttt aactgctgcg
     4201 tattattata ttcaagagtt aaagtgtgag cagccacact cgctggaacg ccaccagaat
     4261 ctggctcgcc aaaagtcagc aagcctgaac ctgctttaac taacaagcca tcagaatggc
     4321 cgtgatagca accctcaaac ttcacaattt tatcgcgccc ggtaaacccg cgcgcggtgc
     4381 ggattgcact catggttgct tctgtgccac tgctatttag gcgcacttgc tccatgctcg
     4441 gcactagctt gttaatcaac tgcgccattt caagctctaa accagttggc gcgccaaagc
     4501 tcaagctatt ggccgccgct tcttgcacag cgctaattac ggctgggtgc gcgtggccta
     4561 caatcatcgg cccccaagaa ttgatatagt cgatatattc tttaccgtcc acatcccaaa
     4621 gcttagaacc caaccctttt ttaaagaaga ttggtgtgcc gccaacacta cgaaaagcgc
     4681 gcactggtga attaacacct ccgggaataa gcgcttgaga tttttcaaaa agggtttggt
     4741 tttgtgagat tgctggtgcg gtcatgatgt catcttaaat tacgaataaa aatctatttt
     4801 aacagagtgt tctagccgtg ttgtaaacct tctttttaag cgctttttta gcacaaccct
     4861 ttttacatgc tctgtaagcc tttatttata aggctcgcag agcatcaaaa ttttctcggg
     4921 gaaaataacg gctcaataga tggctcgttt cagtaatatt ttttgcatta aacagtgcat
     4981 ttaccactgc aatggcatct gcacccgctt gcaaaacctg ttgtgcattt tctagggtaa
     5041 ttccaccaat ggccaccatc ggcacatgaa tgtgctgtcg agcttcttta aaaaggctta
     5101 gcggtgcatg gcgagcgttg ggtttggtgt tagagccaaa acacgcgcca aatgccacgt
     5161 aatctgcgcc ggctgattgc gcctgcgtag caaggtcaat gcggttataa caagaggcac
     5221 ctaaaatttt gcctgcgcca agccgcgcgc gcgcttcttt taaatcgcca tcttctgcgc
     5281 ctaaatgcac accatccgca tcgagcgtta agcaaagttt aattgagtcg ttgatgatta
     5341 acggcacacc atattgccga cacaaaggta aaagcgcgcg cgcttgctgc gttttcaact
     5401 tatgattagc ggctttattg cgatattgca gcaaactaac gccaccttgc agtgctgctg
     5461 ttactttttg tattagcagc gctgtatcgg gctcgtctag agttacggcg tataagcccc
     5521 tgattttatg cggagaattc aatctgaatg ctttaattaa tgtgcttttg tcgtcacatc
     5581 gccactggta accacggcgt cttcttcatc acgcgcccaa aacaagcggt caggaatata
     5641 ttgccccatg ccaggtcgaa agccgttttt aagggtttgc caagtaaact cttgtgcttc
     5701 caaaacggct tcttcaagcg tgaggccgtg cgccatgcaa gcggctatgg cgcttgtgag
     5761 cgtgcagcct gagccatgat agctgccaac caaacgttcc catttatagg ctttaacaaa
     5821 attattttcg ccatacagcg tattggtcac ttctaacgtg cgctcgtgtg tgccggtaat
     5881 tagcacatat tcactgccca tcgctaataa tctagccgcg ctttcatcca gtgaagtcag
     5941 cgcgacttca tcgctatctt cagcaaaagc taatcggcgc gcttctaaac tattgggtgt
     6001 aattaaagtt gcttgcggaa aaagcaactc aatcatcgcc tccatcattt catcattact
     6061 taaatcatcc ccacggccag aggttaaaat ggggtcaatt aacaaaggca catctgggta
     6121 atcggccata atttctgcaa tcactgcaat attttcaact gagcctaaca gcccaagctt
     6181 aaacgcggaa acttgcacgt cttctaaaat ggcacgcgct tgatcgttca cccattcagc
     6241 gtccatggcc aatacaccat tgacgccaat ggtatcttgc gctgttattg ctgtcaccac
     6301 agatagcgga tggcagccaa tgcttgcaaa tgtcagaata tctgcctgca aacctgcgcc
     6361 actgctaggg tcagtgcctg caaaagtgag tacggatggt ggtgattgat tactcataag
     6421 taataacttt attggtgaag atggagcggg cgatgggaat cgataaacgc cctacaaccc
     6481 tcttatttac tgcatcgtta aaaaaccgtg ttaattttta tacggatttt tatacgtatt
     6541 aaattttgct tacctatttt aaagcacttt aaattggact aaaaaagcag ataaacgccg
     6601 ttatgtaatt gtacctaatg cgggtgcaat tatctgcata agatttaatc attaatcttt
     6661 ttatcttgct tggccgcttt tgttggtggt gtaagtagct caccagcgag gtcatgcagc
     6721 ttaagcaaca ggcttaaatg cgattgcgcc cataatggca gcggggcggt accagttaac
     6781 caatgagaca cggccacttt acttaagcca gtggccatag caaagtctgt ttgcttccag
     6841 cctagtgtgg caagcccctc tctaaattca tcagctgtca taacatacct taatcatagt
     6901 aatttgcacc cgctaaaggt aacacgctag aatcgttaaa gcaagtttaa caatagttga
     6961 attatcgtta tagccaaagt ggggaatgat agctaaaaag tgtactaaat ttaacgtaaa
     7021 gtgggaaatt gttaaataaa tgaaatttgt ttcgtataac ggatgttatg taaaattgct
     7081 attttttatt tgtgggaaac tcaataaaat caatgactta cgaaaatgat aatttgcgca
     7141 atatgttatt ttatgcagta ataattgtca ataaccaagc ttttttatat atgtgtatca
     7201 aaatgccgct ttaagttatt agtgatagcg ccccagcgaa ttaacaaaat atactagtcg
     7261 tttttaatca aatcatccaa tttaattaaa
//
LOCUS       DAOQSI010000109         7147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_10243_length_7147_cov_6.161027, whole genome shotgun sequence.
ACCESSION   DAOQSI010000109 DAOQSI010000000
VERSION     DAOQSI010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 7147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7147
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10243_length_7147_cov_6.161027"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..174
                     /locus_tag="PKL42_07450"
     CDS             <1..174
                     /locus_tag="PKL42_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNU66948.1"
                     /translation="AKHMHTIISSECTGCELCVAPCPVDCITMEPIAETPDNWKWKYP
                     VIPIRAVPLHEVK"
     gene            202..2052
                     /gene="rsxC"
                     /locus_tag="PKL42_07455"
     CDS             202..2052
                     /gene="rsxC"
                     /locus_tag="PKL42_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HNU66949.1"
                     /translation="MNAILNFFKPDFLKQKRGVHPPEHKAESTQLPIAKLAMPQKLVL
                     PLRQHIGNMPKILVSVGEHVLKGQLLATPECTVSAAIHAPTSGTILSIADEIIPHPSG
                     LPDKCITLIPDGKDQWTNLTSLDWRNAPKEALIASLCTSGIVGLGGATFPTHIKLRSS
                     GKLNIQTLVINAAECEPYITCDDMLMREHAEEIVQGIEIAQYLLGAEKSVIGIEDNKS
                     IAQQAMQAALTGKPISIVTVPTLYPSGDARQLIHLVSGIEIPHDKRATEMGVQVFNVA
                     TVRALYRHFTFGEPAINRVVTVTGNVAQPQNFEVLFGTPLTALTQATGGAKPDTTHYI
                     MGGPMMGFDLPTPEVPITKATNCIIAASPHLFAPPPPAMPCIRCARCAEACPIQLQPQ
                     ELYWFAKSDAFEKARDYKLFDCIECGVCTYVCPSNIPLVQYFRYAKSEVIAADKAKEA
                     ADLARERNEFRLARIEREKQERAQKHAERALGTKAEAAKAASAKKDAANEAPTTEAPI
                     NSATDEAAKAAKQAAIAAAIERAKAAKAAADATGLAPKNTAPANPAVQQEIAEIDARR
                     KNVGLANTNEAPMTADAEKQAKIAAAIARAKAAKAEAEAAKQAQNLELKE"
     gene            2054..3073
                     /gene="rsxD"
                     /locus_tag="PKL42_07460"
     CDS             2054..3073
                     /gene="rsxD"
                     /locus_tag="PKL42_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HNU66950.1"
                     /translation="MNRSPYISDAPSVSMIMLKVLLALVPGIFAYAWIYGGGILVTIA
                     LATLTALAAEAALLKIRQRPIKPHLMDLSAVVTAWLLALALPPLAPWWLIVVGTLFAI
                     VIAKQLYGGLGYNPFNPAMVGYAVLLISFPLIMTKWPAPLILAQTKLSFLDQLYYIFN
                     QVLPNDIKVDAITSATPLDYLKTHLKLNQTLSEISQAPIFGVYGAKGGEVVTGAYLLG
                     GLYLIQQRIIGWHLPTAFLGALSAIALVFYVANPEHFASPLFHLTSGASLLCAFFIIT
                     DPVSGPTTPRGKLYFAAGVGVLTYLIRVYGGYPDGVAFAVLIMNMCVPLIDAHTQPRV
                     FGHEK"
     gene            3075..3743
                     /gene="rsxG"
                     /locus_tag="PKL42_07465"
     CDS             3075..3743
                     /gene="rsxG"
                     /locus_tag="PKL42_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HNU66951.1"
                     /translation="MSKLFFKNPIVKHAVQTALSLLAFAFVGTLMLAYVFDITRAPIE
                     ASEKEARLALFAQILPKGNFDNDLLKTTVQIPANAQLGNLQPSTANVAKINNKTVAVI
                     LEAIAHDGYSGDIKLLIALRADGTISGVRVLAHKETPGLGDYIDIAHGDWIKLFNNES
                     VHKTPEAQWQVKKDGGKFDYMVGATITPRAVVKAVLKATQFFEANKATLFSIQTTQAE
                     KLAQ"
     gene            3984..4670
                     /locus_tag="PKL42_07470"
     CDS             3984..4670
                     /locus_tag="PKL42_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HNU66952.1"
                     /translation="MSNIYKEIVENGLWKQNPGVVQLLGLCPTLAVTTTAVNGISLGL
                     ATALVMAASNGAVSPVRKWIASEIRVPVFILVIAALVTVIDLSINAFAHPLHKVLGIF
                     IPLIVVNCIVLARVESFAAKNAPVPSILDGLMMGIGLTLVLALLGAMREIFGRGTLFS
                     GLDLVFGPAAKQFILTVIPDYHGFLLAVLPPGAFLGLGILIAGRNWLELRKAAKTSAP
                     THPLKPALSH"
     gene            4697..5332
                     /gene="nth"
                     /locus_tag="PKL42_07475"
     CDS             4697..5332
                     /gene="nth"
                     /locus_tag="PKL42_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNU66953.1"
                     /translation="MNAEKRFEIFNRLKLAIPNPATELKHNSTFELLIAVILSAQATD
                     KSVNLATEKLYAVANTPETILALGLQGLESYIQSIGLYHAKAKNVLATCQQLISQHQS
                     QVPNTRAALEALPGVGRKTANVMLNTAFGQPTIAVDTHLFRLGNRIKLATGRTVLEVE
                     KKYLKTIPQAFMQDAHHLLILHGRYTCTARKPKCSSCCIEDLCEYNAKEYH"
     gene            5359..6471
                     /locus_tag="PKL42_07480"
     CDS             5359..6471
                     /locus_tag="PKL42_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HNU66954.1"
                     /translation="MKVATSIVIGRTANPNLAAQAVMQAMHKADISIANSVLLLLSSE
                     FANNPQPAIRAAAKAGNCTQVFGCSAAGIFTEDDWVLDAPAAAAMVFSGDVGIQPAKS
                     NLTQQSQQALLTLTAPNAINSTWLNDGHDRYGGVSGDVVGQGPFSVWQNSKGESAGRV
                     EAIFSGVKLATSASHGLKILTKPKKIQSTETFDITQLDNKPPFFALQKAWEAESKGKP
                     AAKDATLPLHLMMAVYANSVEAIMTGEFNQTHLISFDETVGSVTLAQTLQPGQFLSWG
                     IRDSETAQQDLRQITQQLTENLGSKPDFGLLFSCIGRGPFYDGIDHDLNIITQQLPNM
                     PLLGFYGNGEISRIGEHNQLLPYSAVLSLFAAHHTK"
     gene            6468..6902
                     /locus_tag="PKL42_07485"
     CDS             6468..6902
                     /locus_tag="PKL42_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HNU66955.1"
                     /translation="MSLFNPSRDQVRQFFFNTWQQFSQQKPLTDLEKIALEVIQMHPE
                     YHAILSHPAQTSQQEYFPEMGETNPFLHMSLHLSVIEQIGINQPIGIASIYESLKAKH
                     QEAHHAQHDILDCLAETIWQAQRNQRPLDAEHYLTLLNKKLM"
BASE COUNT         1980 a         1736 c         1644 g         1787 t
ORIGIN      
        1 gctaaacata tgcacaccat tattagtagt gaatgtacag gttgcgaact ctgcgtagcg
       61 ccctgccctg tggattgcat taccatggaa ccgattgctg aaacgcctga caactggaaa
      121 tggaaatatc ctgtcattcc cattcgcgcc gtgccgttac atgaagtgaa atgaaatgcg
      181 tcttaaacca gtgaatgata tatgaacgct attctgaatt tttttaagcc agacttttta
      241 aaacaaaagc ggggcgtgca tccgccggag cataaggcgg aatcgacgca actgccgatt
      301 gccaaattag ccatgccgca aaaactggtg ctgccgttgc gccagcatat tgggaatatg
      361 cctaaaattt tagtcagcgt gggtgaacat gtactaaaag ggcaattact cgccacgcca
      421 gaatgtacgg tttccgcggc gattcacgcg cccacttctg gcactatttt atctattgca
      481 gacgaaatta ttccacatcc ttcagggctg cccgataaat gtatcacgct gattcctgat
      541 ggaaaagacc agtggaccaa tttaacttcg cttgactggc gtaacgcacc aaaagaagca
      601 ctgattgcca gtttatgcac ctctgggatt gttggtttag gcggcgctac ttttccaacc
      661 catattaaat tacgcagcag cggcaaatta aacattcaaa ctttagtcat taacgcggcg
      721 gaatgtgaac cctacatcac ctgcgacgac atgctgatgc gagaacatgc cgaagaaata
      781 gtacaaggca ttgaaattgc gcaatattta ttgggcgcag aaaaaagcgt aattggcatt
      841 gaagataata aatccatcgc ccagcaagcc atgcaggccg cgctgacggg caagccaatt
      901 tcaattgtca cggtgccgac cctttacccc agcggcgatg cgcggcagtt gattcatctg
      961 gtttctggca ttgagattcc gcatgacaaa cgtgcaaccg aaatgggcgt tcaggtgttt
     1021 aatgtggcca ccgtgcgggc gctttatcgg cattttactt ttggtgaacc cgctatcaat
     1081 cgggtggtga cagtcacggg caacgtcgcc cagccacaaa attttgaagt attatttggt
     1141 acgccgttaa ctgcgcttac acaagccaca ggtggcgcaa aacctgacac aacgcactat
     1201 attatgggcg gccccatgat ggggtttgac ttacccacac ccgaagtgcc cattaccaaa
     1261 gccaccaact gcattattgc ggcttcaccg catttatttg ccccgccacc gccagccatg
     1321 ccttgcatac gttgcgcccg gtgcgcagag gcctgcccga ttcaattgca accgcaagaa
     1381 ctttactggt ttgccaaatc ggacgctttt gaaaaagccc gggattacaa actgtttgac
     1441 tgcattgagt gcggcgtgtg cacgtatgtg tgcccaagca atattccgct tgtacaatat
     1501 tttaggtacg caaaaagtga agtgattgcc gctgataaag ccaaagaagc ggccgattta
     1561 gcgcgcgaac gcaacgaatt caggctggcg cgtattgagc gagaaaaaca agaacgcgca
     1621 caaaaacatg ctgaacgtgc gctgggtaca aaagctgaag cggctaaggc tgcaagtgcc
     1681 aaaaaagacg ctgcaaatga agccccaaca acagaagcac cgataaacag cgcgactgac
     1741 gaggccgcca aggcggcaaa acaagcggcc attgccgccg caattgagcg ggcaaaagcg
     1801 gccaaagctg cggccgatgc aactgggtta gcacctaaaa acacggcgcc agccaacccc
     1861 gctgtgcagc aagaaatcgc tgaaatagat gctcggcgaa aaaatgttgg tctagccaat
     1921 acaaatgaag cgccaatgac ggcagatgct gaaaaacaag ctaaaatcgc cgcagccatt
     1981 gcgcgggcga aagcagccaa agcggaagcc gaagctgcaa aacaggcgca aaatttagaa
     2041 ttgaaggaat aacttgaacc gctctcctta catttcagac gcacccagtg ttagcatgat
     2101 tatgctaaaa gtattgctgg cgcttgtgcc gggcattttt gcttatgctt ggatttacgg
     2161 tggcggcatt ttagtcacca ttgcactcgc caccctcacg gcgcttgctg ctgaagcggc
     2221 cttattgaaa attcgccaac gcccaatcaa accccatctc atggacttaa gcgcagtggt
     2281 caccgcttgg ctattagcat tagcgctgcc gccgcttgcg ccttggtggt tgattgtcgt
     2341 cggcacgctg tttgccattg tgatcgccaa acaactttat ggcggcttag gctacaaccc
     2401 atttaacccg gcgatggtgg ggtatgcggt gttattgatt tcatttccgc tcattatgac
     2461 taaatggcca gcgcctttaa ttttggcgca aacaaaatta agttttttag accaactcta
     2521 ttatattttt aaccaagtgc taccaaacga tatcaaagtt gatgcgatta cctcggctac
     2581 gccattagat tatctaaaaa cacatctcaa actgaatcaa accttgtccg aaatatctca
     2641 agcgcccatt tttggcgtgt acggtgccaa aggcggcgaa gtcgtcacgg gcgcctattt
     2701 gcttggcggt ctttatttaa ttcaacagcg cattatcggc tggcatttac caacggcatt
     2761 tttgggtgcg ctatcggcga ttgccctggt tttttatgtg gctaaccccg aacactttgc
     2821 cagcccgtta tttcatttaa ccagcggtgc atcactactt tgtgcatttt ttattatcac
     2881 cgatcctgtg agcggcccca ccacaccgcg tggaaaactc tattttgctg cgggcgtcgg
     2941 tgtgctgact tatctaattc gcgtttatgg cggctaccct gatggcgtag cgtttgcggt
     3001 gttgatcatg aatatgtgcg tgccgctgat tgatgcgcat acgcaaccta gagtgtttgg
     3061 ccacgagaaa taacatgtcg aagcttttct ttaaaaaccc gattgttaag cacgctgtac
     3121 aaacggcgct ctcgttattg gcgtttgcct ttgtgggcac actcatgctg gcttatgtgt
     3181 ttgacattac gcgcgcgcct attgaagcga gtgaaaaaga ggcgcgtttg gcgctttttg
     3241 cgcaaattct gccaaaaggc aatttcgata acgatttgtt aaaaaccact gtgcagattc
     3301 cagccaatgc gcagctggga aacctgcaac caagcacggc caatgttgca aaaatcaaca
     3361 acaaaacagt ggccgtgatt ttagaggcca ttgcccatga tggttatagt ggcgacatta
     3421 aattactcat cgcgttacgc gctgacggca ccattagcgg tgtacgcgta ttagcgcata
     3481 aagaaacacc cgggctgggt gattacattg acattgctca cggcgactgg attaaactat
     3541 tcaacaatga atctgtccat aaaacacctg aagcgcaatg gcaggttaaa aaagatggcg
     3601 gtaaatttga ttacatggtg ggtgcaacca ttacgccccg cgcggtagta aaggccgtgt
     3661 taaaagccac gcaatttttt gaggccaaca aagcgactct tttttcaatt caaacaacac
     3721 aagctgaaaa attagcccag taaaatacgc tgaaatattt tgcaaaaagc agtgattatt
     3781 ttgaattgaa aattacattt tacgcattaa aagtaacaca acccttttac gatgctctgg
     3841 gagccttgta aataaaggct cacagggcat aaaaaaatcc tcggcggatt ttaggtgctt
     3901 tatgaatgtt ttttgcgcaa gaattaaagt aaaatttcag gcataaacta cgccgtttgg
     3961 cgcaagtttt tgaaggataa aatatgagta acatttataa agaaattgtc gaaaatggct
     4021 tatggaagca aaaccccggg gtggtgcaac tcttagggtt atgccctacg ttggctgtca
     4081 ccaccacagc cgtaaatggc ataagtttag gcctggccac ggctttagtc atggccgctt
     4141 caaacggtgc cgtgtctccg gttcgcaaat ggattgcgag tgaaattcgc gtgccagtgt
     4201 ttattttagt cattgccgcg ctcgtgacgg tgattgattt atccatcaat gcgtttgccc
     4261 acccgctaca caaagtgctg ggcattttta ttccgctgat tgtagtcaat tgtattgtgc
     4321 tggcccgcgt tgaatcattt gccgctaaaa atgcgcctgt gccttcaatt ttggatggct
     4381 tgatgatggg cattggttta acgctggtgt tagctttgct gggtgccatg cgtgaaatat
     4441 tcggtcgcgg cacgctgttc tcagggctgg atttagtctt tggtccagct gccaagcagt
     4501 tcatattaac cgtaattcca gattaccatg gctttttact ggccgtgttg ccaccgggtg
     4561 cttttttggg gctgggcatt ttaattgcag ggcgtaactg gcttgaactc agaaaagccg
     4621 ccaaaaccag cgcgcctaca catccgctaa aacctgcatt aagccactaa atatcatcac
     4681 ctcatttaag tgacttatga acgctgagaa acgctttgaa atttttaacc gcctaaaact
     4741 cgccattccc aaccccgcca ctgagctcaa acacaacagc acatttgagt tgctgattgc
     4801 cgtgatttta tctgcccaag cgactgataa aagcgtgaac ctcgccacag aaaaactgta
     4861 tgccgttgcc aatacgcctg agaccatatt ggcattaggg ctgcagggct tagaaagcta
     4921 tattcaatcg attggtttat atcacgcgaa agccaaaaat gtgctggcca cttgccagca
     4981 actcatcagc cagcatcaat cacaagtgcc taatacgcgc gccgctttag aagcacttcc
     5041 cggtgtgggc cgaaaaacgg cgaatgttat gttaaatacc gcttttggcc agccgaccat
     5101 tgccgtggat acgcatttat ttcgtttagg taaccgcatt aaactggcca cgggcagaac
     5161 ggtgctggag gttgaaaaaa aatacttaaa aacaattcca caagctttta tgcaagatgc
     5221 gcaccacctg ctgattttgc atggccgcta tacctgcacg gcgcgtaagc caaaatgcag
     5281 cagttgctgt attgaagatt tatgcgaata taatgctaag gaataccatt aagcccatat
     5341 ggaaacacaa agaacctcat gaaagttgcc accagtatcg tcattggccg cacggctaac
     5401 ccaaaccttg ccgcacaagc cgtaatgcag gccatgcaca aggccgatat ctcgattgcg
     5461 aatagcgtat tactgctatt gagttcagag tttgccaata atccgcagcc tgctattcgc
     5521 gctgcggcca aagcgggtaa ctgtacgcag gtcttcggct gttctgccgc aggcattttt
     5581 acagaagacg attgggtgct ggatgcgccc gccgctgccg caatggtgtt tagcggagac
     5641 gtaggtattc aaccggccaa atcaaacctc acacaacaat cacaacaggc actgctcacc
     5701 ctcaccgcac ctaatgccat caacagcact tggctaaatg atggccatga ccgctatggt
     5761 ggcgtttctg gcgatgtggt tgggcaaggg cctttttcag tttggcaaaa tagcaaaggt
     5821 gaaagcgcag ggcgggtaga agccattttt tcaggcgtaa aacttgccac gagtgcatcg
     5881 cacggtttaa agatactgac caagcctaaa aaaattcaaa gtactgagac ctttgatatt
     5941 acgcaacttg acaataaacc accctttttt gcccttcaaa aagcctggga ggccgagagt
     6001 aaaggcaagc cagccgccaa agacgccaca ctaccgcttc atttgatgat ggcggtttac
     6061 gccaatagtg tggaggcgat catgacaggc gaattcaacc aaacacattt aattagtttt
     6121 gacgaaaccg taggcagcgt aacgctcgca cagaccttgc agccaggcca atttttaagt
     6181 tggggcattc gtgatagtga aacagcgcaa caagacttgc gccaaatcac gcaacagctc
     6241 accgaaaact taggcagcaa gcccgatttt ggactattgt tttcatgtat tggtcgcggc
     6301 cctttttatg atggcattga ccatgactta aatatcatta cacagcaact ccccaatatg
     6361 ccgctgcttg gcttttatgg caatggcgaa atttcgcgga ttggtgaaca taatcagttg
     6421 ctaccctact cagccgtctt aagtttattt gcagcgcatc atacaaaatg agtttattta
     6481 accccagccg cgatcaggtt cgccagtttt ttttcaatac ctggcaacaa tttagccaac
     6541 aaaaaccgct cactgattta gaaaaaattg cgcttgaagt cattcaaatg catcctgaat
     6601 accacgccat tttgagtcat ccagcgcaaa ctagccagca ggaatacttt cctgaaatgg
     6661 gcgaaaccaa tccattttta cacatgagcc tgcatttgtc tgtcattgaa caaattggca
     6721 tcaatcagcc cattggtatt gccagcattt atgaaagcct caaggccaaa catcaagaag
     6781 cacatcatgc ccaacatgat attttagatt gtttggccga aaccatttgg caggcgcaac
     6841 gcaaccagcg cccgttagat gcagaacatt atcttacttt actcaacaaa aagttgatgt
     6901 gagtccgcgt gaaaatatag cttaaaacct gcttaaaaat caccgagaga aaaacactgc
     6961 cctgcgagcc tttatttata aggctcgcag ggcagtgcaa aaggttgtaa cttaaacagc
     7021 ataaaaatat ttgccttttt aatgcaacaa cagtaataat gcaacacccc aataaggaag
     7081 ctcgatgacc agcacagcac acgaatggct taacgcgcat ggcgattatt tataccgctt
     7141 tgcgctg
//
LOCUS       DAOQSI010000110         7144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_10248_length_7144_cov_5.559458, whole genome shotgun sequence.
ACCESSION   DAOQSI010000110 DAOQSI010000000
VERSION     DAOQSI010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 7144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7144
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10248_length_7144_cov_5.559458"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..1076
                     /locus_tag="PKL42_07490"
     CDS             36..1076
                     /locus_tag="PKL42_07490"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNU66956.1"
                     /translation="MGNENQASVADRRRPSLLSNTALILVIIALLTLGWQWITTQQKF
                     SAVEKTLSQRLENYQALNQQSLALAKQAEERSTQANARTVLLEEKIAESRDQQEVLQT
                     LYNQLAENREATAVAEVEQLLTIANQQLQLAGNIKAALLALEAADKRLQPLALPRASQ
                     LRQTLALEIENLRKLPQVDISNISLQLERLSQLSATLPLISERQPTLNASLAELETPQ
                     ASNLNAVEKVVLPIWRDIKNLVTVERIDKPEPPLLPADNARYLRENIKLRLLTARIAL
                     LQRDEATYKADLTAVVDWLNLYYDGKHPKTKQAIAQLKELSNSDINIELPQLNESIGA
                     VNRYKQTLEDHD"
     gene            1069..1491
                     /locus_tag="PKL42_07495"
     CDS             1069..1491
                     /locus_tag="PKL42_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HNU66957.1"
                     /translation="MIRRKIFWWLLMFALLVCVVWFASNNEGYVLIVRPPYRLQFSFN
                     FLLILMVLGFLAIHYCLRFLHFLRRFPANRRSKQETQRLEASNAALLQGMHALAEGDF
                     ESAEIAVKHAHDLIQNADHERLIAALAAEKNKQSTLFN"
     gene            complement(1672..2706)
                     /locus_tag="PKL42_07500"
     CDS             complement(1672..2706)
                     /locus_tag="PKL42_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HNU66958.1"
                     /translation="MTRITIQSSNHQFEARPSQTILEAAIAAGVNLPYGCRSGACGNC
                     KGDIISGEIDYGEYAASALTDAEKAAGKALFCCAHLLTDVVIACREIASDVIPPRILP
                     TRVERKVQLSHDVMALFLKLPSNEHLQFRAGQYIEFLLKDGKRRAFSIANAPHVDNLL
                     ELHLRLIPGGQFTEYVFNEMPEKAILRIEAPFGSFFLREDSTKPIIMVAGGTGFAPIK
                     GIIEHMLQHHIQRDVILYWGALSKQDLYMPELPQAWAAQHPHIKFIPVLSDAKPEDQW
                     TGRTGYVHQAVLDDFAATGLAEFEVYCCGAPAMVEVAHASFMQAGLAEDAFLSDAFTY
                     AKPLPNKTTS"
     gene            complement(2703..3182)
                     /locus_tag="PKL42_07505"
     CDS             complement(2703..3182)
                     /locus_tag="PKL42_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HNU66959.1"
                     /translation="MRLILFLMLLAFPIAEIWILINLSHRYGWWVLLYLVVVGWLGLQ
                     LVREEKQLFSGKMMQSLMQGGNPARAMFASARNLIAGVLLMIPGVLTDVIAAILLLIP
                     MKQPKLASGRSDSQTYQEPTYQQEQYQEKTFKHQRKASNDEIIDAEFTEIKEETDKK"
     gene            3428..5230
                     /gene="dsbD"
                     /locus_tag="PKL42_07510"
     CDS             3428..5230
                     /gene="dsbD"
                     /locus_tag="PKL42_07510"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HNU66960.1"
                     /translation="MLNALIDDGAETFLPPEVAFKLALKGMDANHIEASFLVQPKYYL
                     YRERIKFEVVSPAEVKISQITLPNGDIKDDPNFGKQEVYHNDFKANIQLSAGTQSPVT
                     INASYQGCSEKGLCYAPIKQTFTLNLPANAALASTSSVSSAANNLTASNEVDSTTQIL
                     KSGNLWLIIAGFFVAGLLLSFTPCVLPMIPILSSIIVGSQSQQVRLSRLHAFMLSVAY
                     VLGMALSYTLAGIAAGLSGDLLSQSLQNAWVLGATALIFVLLALSMFGFYELKLPQSF
                     EDKLVLTTNKLKGGHFLSVFIMGALSALIVSPCVAAPLAGALIYIGQTHNVFLGGVGL
                     FALAIGMGVPLLIIGASAGSLLPKTGNWMNSVRNFFGVLMLGMAIWLISPVIPVNVQL
                     ALWAALLIVTAVYHNALDQLPAHANSVAKVGKGLAVIALLFGVAFLIGALSNAKSVVQ
                     PLSGFSFSTTKQQNSLPPLAFTRITTLAELDQKLAETGGKPVMLDFYADWCVACKELE
                     AFTFSHPQVQQLLKNTTLLQVDVTANSDEDKALLKRFGLFGPPGIAFFNAQGHEMTTL
                     KTVGFQNADRFSDTLARRDRCIVTPLSNGKTLQC"
     gene            5239..5784
                     /locus_tag="PKL42_07515"
     CDS             5239..5784
                     /locus_tag="PKL42_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNU66961.1"
                     /translation="MTTLGYLAIMLSLGFVIYFYFLNPNSPLKNLANDFSALTTKPLF
                     AAKFPNENGVIQDLNQYKGKIIVLNFWATWCPPCREEMPDFSKLYQNYQHQNVVVLGA
                     AADELAAVRHYLAASPVSYPIIVGDMEAMALASNLGNAQGALPFTVFINTDGIVANVH
                     LGRISYTQLEKELTRILPSKN"
     gene            6031..6480
                     /gene="aroQ"
                     /locus_tag="PKL42_07520"
     CDS             6031..6480
                     /gene="aroQ"
                     /locus_tag="PKL42_07520"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNU66962.1"
                     /translation="MATKSILVLHGPNLNLLGLREPNHYGHATLASINQALSDKAAVA
                     GVALETFQSNAEAELVNKVQSIASKSVDFVIINPAAFTHTSVALRDALSAVKVPFIEV
                     HLSNVYAREAFRHHSYFTDIAVGVISGLGAQGYELAFDYAIRHVHQA"
     gene            6525..6980
                     /gene="accB"
                     /locus_tag="PKL42_07525"
     CDS             6525..6980
                     /gene="accB"
                     /locus_tag="PKL42_07525"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNU66963.1"
                     /translation="MDLRKLKKLIDLVEESGIAELELTEDGEKVRISRNFTANNNVPT
                     QQYYAQYAPPAQQQPQISQAPAVEAPAVETGHTVKSPMVGTFYRASSPDAKSFVEVGD
                     TVAVGDTLCIIEAMKLLNEIESDKAGVVKKILVDNGLAVEYGEPLFIIG"
     gene            6998..>7144
                     /locus_tag="PKL42_07530"
     CDS             6998..>7144
                     /locus_tag="PKL42_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNU66964.1"
                     /translation="MFEKTLPASRIEKILIANRGEIALRVQRACRELGIRTVAVHSEA
                     DREAK"
BASE COUNT         1971 a         1624 c         1544 g         2005 t
ORIGIN      
        1 gataattcaa cgattggcgc aattggtgaa acctcatggg aaatgaaaat caagccagcg
       61 ttgcagatag gcgccggcca tcgttgctgt ctaacacggc gctcatttta gtcattattg
      121 ccttgctgac gctaggctgg caatggatta ccacgcaaca aaaatttagc gccgttgaaa
      181 aaaccttaag ccagcgacta gaaaattacc aagcgcttaa ccagcaaagc cttgcgttag
      241 ccaaacaggc tgaagagcgc agcactcaag ccaatgcccg cactgttttg ctagaggaaa
      301 aaatcgccga atcgcgcgac cagcaagagg tattacaaac cctttacaac caactggcag
      361 aaaaccgtga agccaccgca gtggcagaag tagaacaact cctcaccatc gctaaccagc
      421 aacttcagct tgctggcaat attaaagccg cgcttttagc acttgaagca gccgataaac
      481 gtttgcagcc gctggcgttg ccgcgcgctt cacaattgcg ccaaacgctg gcgcttgaaa
      541 ttgaaaatct gcgtaaatta ccgcaggtgg acatcagcaa tatcagtttg caattagagc
      601 gtttaagcca attaagtgcc acgctgccgt taatcagcga gcgccagcct acgctcaacg
      661 ccagccttgc cgaacttgaa accccgcaag ccagcaacct caatgcggtt gaaaaagttg
      721 tgctgcccat ttggcgtgat attaaaaatc ttgtcaccgt tgagcgcatt gataaacctg
      781 agccgccttt actgccagcg gataatgcgc gctatttgcg tgaaaatatc aaattgcggc
      841 tgttaactgc gcgcatcgcc ctgctgcaac gcgatgaagc cacctataaa gccgacttaa
      901 ctgccgtggt ggactggctg aacctttatt acgatggcaa acaccccaaa accaaacagg
      961 cgatcgctca actgaaagag ctttctaaca gcgacattaa cattgaatta ccgcaactaa
     1021 atgaaagcat tggcgcggtc aatcgttata aacaaacgct agaagaccat gattagacgc
     1081 aaaatttttt ggtggctgtt gatgtttgca ttgctggtat gcgtggtgtg gtttgccagc
     1141 aataacgaag gctacgtact catcgtgcgc ccgccctacc gcttacaatt ttcttttaac
     1201 tttttgctga tattaatggt gctgggcttt ttggccatcc attactgttt gcgatttttg
     1261 cattttttgc ggcgctttcc agccaatcgg cgcagtaaac aagaaacgca acggttagaa
     1321 gccagcaatg cggcactttt acaaggcatg cacgcactag ctgaagggga ttttgaaagt
     1381 gctgaaattg cagtgaagca cgcacatgat ttgattcaaa atgctgacca tgaaagactc
     1441 attgcagcgc tggcggctga aaaaaataaa caaagtacgc tgtttaacta gccaaaaaac
     1501 ctgttttttg tgtgcaaaat ttagcgttca aaaacattgt ttttttactg aaatttcccc
     1561 gagaaaaaat ttatgctctg gaagcctttt tttataaggc ttccagaggc atcaaaaagg
     1621 ttggtgttaa attaagcgct ttttaagctt aaaaatacat gcataaaaca cttatgatgt
     1681 tgttttattt ggcaatggtt ttgcgtaagt aaaggcatcc gataaaaacg catcttctgc
     1741 caaacccgct tgcataaaac tggcatgcgc cacttctacc atggcgggtg cgccacagca
     1801 ataaacttca aattcagcta ggcccgtagc ggcaaagtca tccagcaccg cttggtgaac
     1861 ataaccggtt cggccagtcc attggtcttc aggtttggca tcggataaca cgggaataaa
     1921 cttaatgtgt ggatgctgcg cagcccaggc ttgaggcaat tctggcatgt agagatcctg
     1981 cttagaaagt gcgccccaat acaaaatgac atcgcgctga atgtgatgtt gtagcatgtg
     2041 ctcgataatg cccttaatgg gtgcaaaccc tgtgccgcca gctaccataa tgatcggttt
     2101 tgttgagtct tcacgcagaa aaaaactgcc gaacggcgct tcaatccgca aaatcgcttt
     2161 ttccggcatt tcgttaaaga cgtattctgt gaattggcca cccggaatca aacgcaaatg
     2221 cagctctagc agattatcca catgcggcgc attggcgatt gaaaaagcac ggcgtttacc
     2281 atctttgagc aaaaactcaa tgtattgccc cgccctaaac tgtaaatgct cgttactcgg
     2341 caattttaag aataacgcca tcacatcatg tgaaagttgt actttacgct ccacgcgcgt
     2401 gggcaaaatg cgtggaggga ttacatcaga ggcaatttcg cggcacgcta tcaccacatc
     2461 ggtcagcagg tgtgcgcagc aaaacagcgc ttttccggcg gctttttctg catcggttaa
     2521 ggcgcttgcg gcatattcgc cgtaatcaat ttcgccactg atgatgtcgc ccttacaatt
     2581 gccgcacgcg ccacttctgc aaccataagg cagatttaca cctgcagcaa tagcggcttc
     2641 taatatggtt tggctcggac gcgcttcaaa ttgatgattg ctcgattgaa tggtaatacg
     2701 ggtcattttt tgtcggtttc ttctttaatt tctgtaaatt cggcatcaat aatttcatca
     2761 tttgaagctt ttcgctgatg cttaaacgtt ttttcctgat attgttcttg ttgataagtc
     2821 ggttcttgat aggtttgcga atcacttcga ccgcttgcca atttaggttg tttcattgga
     2881 atcagcaaca atattgctgc aatcacatcg gttagcacac caggaatcat taataaaacc
     2941 cccgcaatta aattgcgcgc actggcaaac atggcgcgtg cagggttgcc gccttgcatt
     3001 aaactttgca tcattttgcc agaaaataat tgcttttctt cacgcaccag ttgtaagccc
     3061 agccaaccga caacgactaa atacaataag acccaccaac catagcgatg acttaagttt
     3121 attaaaatcc aaatttctgc aattggaaaa gccagtaaca ttaaaaacaa aatgaggcgc
     3181 atgtgagagt tttccttcaa atacggaacg taagaccata ttgagtatct ataggttgct
     3241 atctactttt gtggtggcga tgctctataa ttgcaagacc aaacgattac aatagctgaa
     3301 ataattaaat ttggaacata attgcgtgaa aaaattcttg cttaagatgt ttttaaatgg
     3361 cttagcggcg ttatttgcat ttggcttttt ttcaagtgca tacgccgctg aggcaaacaa
     3421 gagctcaatg cttaatgccc tgattgatga tggtgcagag acctttttgc ccccagaagt
     3481 ggcctttaag cttgcactaa aaggcatgga tgcgaaccat attgaagcca gttttttagt
     3541 acagccaaaa tattacttat accgtgagcg tattaaattt gaagttgttt cacctgctga
     3601 agtcaaaatt agccaaatta ctttacccaa tggcgacatc aaagatgacc ccaattttgg
     3661 caagcaagaa gtctaccaca atgacttcaa agccaatatt cagctgtctg ccggcactca
     3721 atcgcctgta acaatcaatg ccagctacca aggatgtagt gaaaaaggct tatgctatgc
     3781 gcccattaaa caaacattca cacttaactt acccgcaaat gccgcactag caagcacaag
     3841 ctctgtcagc agcgcagcta acaacttaac ggctagcaac gaagttgata gcactacaca
     3901 gattttaaaa tctggcaatt tgtggctcat cattgcaggt ttttttgttg cagggttatt
     3961 gctctcgttc acaccttgcg tgttgccgat gattccaatt ttatccagca tcattgtggg
     4021 tagccaaagc cagcaggtac gcctttctcg actacacgcc tttatgctct ctgtcgctta
     4081 tgttttgggc atggccttaa gttatactct tgccggcatt gcggccggat tatcgggaga
     4141 tttacttagc caatcactgc aaaacgcatg ggtgctaggg gctacagcgc tgatttttgt
     4201 gttgctagcc ttgtcgatgt ttggatttta cgaacttaaa ttgccacaat cgtttgaaga
     4261 taaattggta ctcacgacca acaaactaaa aggtgggcac tttttaagcg ttttcatcat
     4321 gggtgcgcta tcggccttaa ttgtgagccc gtgtgttgct gcaccgctgg ctggtgcgct
     4381 gatttatatt gggcaaaccc acaatgtgtt tttgggtggt gttggccttt ttgcattggc
     4441 cattggcatg ggggtgcctt tgcttatcat tggcgcttct gcaggcagct tgttacccaa
     4501 aaccggtaat tggatgaata gcgtgcgcaa cttttttggt gtactcatgt tgggcatggc
     4561 gatttggctc atctcgcctg ttattccggt caatgtgcaa cttgctttat gggcggcttt
     4621 attgattgtt actgcggttt accataatgc gcttgaccaa ctgcctgcac acgccaatag
     4681 cgttgcaaaa gtaggcaaag gccttgcggt gattgcctta ctctttgggg ttgcttttct
     4741 cattggtgcg ctttctaacg caaagtcggt cgttcagcct ttgagtggtt ttagttttag
     4801 cacgacaaag caacaaaaca gtttaccccc actggctttc acccgcataa ccacccttgc
     4861 ggagttagac caaaaacttg cagagaccgg cggtaaaccc gtgatgctgg atttttacgc
     4921 agattggtgt gttgcgtgca aagagcttga agcgtttact tttagccacc cacaagtgca
     4981 gcagttactc aaaaatacca cattgctaca ggttgatgtc accgctaact ctgatgaaga
     5041 taaagctttg cttaaacggt ttggcctatt tggcccgccc ggcattgctt tttttaacgc
     5101 acaaggccat gaaatgacaa cgcttaaaac cgttggtttt caaaatgctg atcgctttag
     5161 cgataccctt gcacggcgcg atcgctgcat tgtcacgcct ctttcaaacg gaaaaaccct
     5221 gcaatgctaa aaaaaatcat gaccacccta ggctatttag ccattatgct cagcttgggg
     5281 tttgttattt atttttactt tttaaaccct aactcaccac ttaaaaatct agcgaatgat
     5341 ttcagtgcgc ttactacaaa accgcttttt gcagctaaat ttccaaatga aaacggtgtc
     5401 attcaagacc taaatcagta caagggcaaa attattgtgc tgaatttttg ggctacttgg
     5461 tgcccacctt gccgtgagga aatgccggat ttttcaaagc tttatcaaaa ttatcaacat
     5521 caaaatgttg ttgtgctcgg cgctgccgct gatgaacttg ctgctgtgcg gcattatttg
     5581 gcggcttccc ctgtgagtta cccgattatt gttggtgaca tggaggcgat ggcgcttgca
     5641 agtaatttag gcaatgcaca aggcgcatta ccatttaccg tttttattaa tacagatggt
     5701 attgtggcca atgtacatct aggccgcatt tcatatactc aactagaaaa agaattgact
     5761 cgcatacttc catctaaaaa ttagatttgc gacctaacta ctcgcttatc attgtctaaa
     5821 tgaagaacca aacgggcgat attttacttt ttatcatctt aaatttactt caattagccc
     5881 cagcaacttt taaaaaaata tcatcgcctt aaatagccaa tttcttaaaa ttcacgctat
     5941 tttactggac aaagttcagt aacttcggca aacttgcagt tatgaattac agttatttac
     6001 aagttgcgtt gcatcgtcat gaggataagc atggcaacta aatccatttt agtgcttcat
     6061 gggcccaact taaatctatt agggctacgc gaacccaatc attacggtca tgcaacactc
     6121 gcttcaatca atcaagcact ttccgataaa gccgccgttg ctggagttgc gcttgagact
     6181 tttcaaagca atgctgaagc tgaacttgtt aacaaagtgc agtctattgc ttcgaaaagt
     6241 gtcgattttg tcattatcaa ccctgcagcg tttacgcata catcggtcgc cctacgcgat
     6301 gcactctctg ctgttaaggt gccttttatt gaagtacact tatccaatgt ttatgcgcgt
     6361 gaggcttttc gccaccattc ctattttacc gatattgcag tcggtgtcat tagtggatta
     6421 ggggcgcagg gttacgagct agcttttgac tatgctattc ggcatgttca tcaagcttga
     6481 taaagattaa atactaaact tttcctggat ttttagaggt tattatggac ttacgcaaac
     6541 taaaaaaatt aattgatctg gttgaagaat caggcattgc cgagcttgaa ctaaccgaag
     6601 acggcgaaaa agtacgcatc agtcgcaact ttacggcaaa caacaacgtg ccaacgcaac
     6661 aatattacgc acaatatgcg ccaccggcac agcaacaacc tcaaatcagc caggcgcctg
     6721 cggtagaagc gcctgctgtt gaaactgggc acactgttaa atcaccgatg gtgggtacgt
     6781 tttatcgcgc atcatcgcct gacgctaaat cttttgttga agtaggtgat actgtggctg
     6841 tgggtgacac gctctgcatc attgaagcga tgaaattatt aaatgaaatt gaatcagata
     6901 aagcaggtgt cgttaaaaag atattggtag ataacggcct agcagttgag tacggcgaac
     6961 ccctcttcat tataggttag gattttataa catggctatg tttgaaaaaa ccttacctgc
     7021 aagtcgtatt gaaaaaatat taattgccaa tcggggtgaa attgctttgc gtgtgcagcg
     7081 cgcatgtcgt gaattgggca ttagaacggt tgctgttcac tctgaagcag accgtgaagc
     7141 caaa
//
LOCUS       DAOQSI010000111         7135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_10275_length_7135_cov_5.042373, whole genome shotgun sequence.
ACCESSION   DAOQSI010000111 DAOQSI010000000
VERSION     DAOQSI010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 7135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7135
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10275_length_7135_cov_5.042373"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1208
                     /locus_tag="PKL42_07535"
     CDS             <1..1208
                     /locus_tag="PKL42_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66965.1"
                     /translation="QISKLYKVAEKHFLQNEAMAIYFDPTSAEQKLTTISSMYIQACM
                     LGSLEGLSFKSQQIELAHKILDFWAAKVSIDRDYDSQKYFFYVDTGSNTPAKRIRKFK
                     PTEHCRYWSLDGINSKVELCLSLLEFNIAPKQEKIKEIVNSKYALITLGALQAEWSRI
                     DPSRQRRSLERVKTEKAANTTFGFEETCDRIRHYESISEHKVAEPKSAEIILTEKDTN
                     HESIKGRGANIIYVDLGAGQSNIVDESLRGFGLLVRKQASELSIGMMIGVSAKEKSHD
                     IKVGVIRNIKPVAGNQFHLGIELLSRAAFCAKAENVSRQLINTTSANQLNDTISGFSN
                     DFTRFTCLFLPKEFSVSKQETLIVPRYQYNRKDAFKINIAGHDMLVRLTDSFEQHENW
                     VRVAFTKAY"
     gene            complement(1389..1961)
                     /locus_tag="PKL42_07540"
     CDS             complement(1389..1961)
                     /locus_tag="PKL42_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66966.1"
                     /translation="MNIKRQAFCMKSHALWVAVFCALASVQVAANESCVAGKVYVDCN
                     HNQRQDLKELGIPNVRIYIEDGTYLITDVAGKFSFCGLSAQTHVLKVDKTTLPTGSQL
                     IASSNRNSGDANSLFLDSKNHQLIRADFIENSCSKAVLTQVRARQNPRIQNTESANQV
                     QPAITFKGTSLEYEVNNPKTSADIAKQKTR"
     gene            2133..2456
                     /locus_tag="PKL42_07545"
     CDS             2133..2456
                     /locus_tag="PKL42_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66967.1"
                     /translation="MQIQTGEIYGKQTAAKFVMYNVLKVSKSVVTLQNIDNPLSLFET
                     TAQKLLSSGYIRISQTPYIDLVSTTPKKRKAAKKPTRCPLTYDFLEARADSERPAQIL
                     TDLFN"
     gene            complement(2467..3921)
                     /locus_tag="PKL42_07550"
     CDS             complement(2467..3921)
                     /locus_tag="PKL42_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HNU66968.1"
                     /translation="MVCNQSNNHPRLKLRLIGLSVVAVLMAGCKTVGPDYQRPEVSLP
                     ATYAEGVSQVSQTIAANWWTHYQDPILNDLVGQALVNNSNIKLAVARIEEANALANEI
                     GAASLPQVDLNGAANRSRVTESGANEVSNNPRNLYRAQLGTSFEIDFWGKLIRAKESV
                     RAQVLASQYAKDTVALSLTGLVASNYLTLRSLASQISVTQANLKTWDESLALTKRRLE
                     GGVASILEVHQAEAASAILSAQLAELTRLRAQSHHQLAVLTGNLNLTLAQVGDVQQLP
                     VPPTPPAGLPSSLLEARPDVAQAEAKMVAANANIGVAKAALYPTISLTAIFGGESMEL
                     ADVLKSASRIWTGGLNLYLPIFDSGKRNARVDQASAKQKQALASYEGAVQNAFREVND
                     ALVDLRQHTARETALNASQQSAKKALEIAENRYQSGYSAYLDVLDAQRVYNETSIAFI
                     QSRQSRLLASVALFKALGGGWNAPDKTNNVVTKQ"
     gene            complement(3921..7064)
                     /locus_tag="PKL42_07555"
     CDS             complement(3921..7064)
                     /locus_tag="PKL42_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HNU66969.1"
                     /translation="MTKFFITRPIFASVLSIIIVLAGLAAAFKLPIAQYPQIAPPTVT
                     ITASYPGASAETLSKTVAAPIEEQLSGVDNLLYFSSSADSSGTLVITATFEVGTDVDQ
                     ATFNVSNRVNIATPRLPDDVRRNGLIVQKKSNDILLVVMLTSKDPKHDRLFLSNYATL
                     NVLDEFKRVKGVGDALIFGGQDYSMRIWLRPDRMAQLGVTTSDIAAAISAQNNQYAAG
                     KIGAEPAPASQQLVYTVTAKGRLVDPEQFANIMIKADGPRGVLYLKDVARIELGAQNY
                     NVTSALNGQTGVALPIFLQSGANALDTAKGIKDKVEELKARFPEGMTYTIPYDTSDFV
                     KATINEVLKTFGEALVLVVLVVFLFLQSWRATLIPIIAVPISLIGTFAGLYMFGFSIN
                     LLTLFAMILAIGIVVDDAIVVLENTERLMREENLAPLPAAIKSIHQVSAAVVAIVLVL
                     CAVFVPVAFQGGIAGELYRQFAVTVAISVVISGIVALTLTPALCAMILKKSHGENWFF
                     TKFNQSFTRFTHFYTHTVGVTLKHKIIGAIAFLGIVATVILLVMKVPGSFVPAEDQGY
                     VVTIVVMPNGATLSRTTKTTENIRAAIHQDDAVAHEFAVNGLELLTGANKTNAATMFV
                     RLKDWQDRTATADDIVGKLFGIGMSQPDGIAFAVNPPAIRGLGSAGGFEVYVQSKLDT
                     DPIKLAEVTNRFIEALTADKNLTGLNTFFRPTVPQLLIEVDEAKALSQKVRISDIYAT
                     LQSTMGSFYVNDFNRNGRTYRVQLQAEQQYRMQPEDLGKVYVRSQPTAVNPAGNMIPL
                     SALSKVTNIVGAEQLERYNGLLSAKVFGSGASGVSSGDAIQTVERIAEANLPEGYQIA
                     WTAQAYQEKRTGSAAMFAFGFAIIMVFLILAAQFETWALPLAVIMAVPFALAGAFLAV
                     LLRGMPNDIYFQIGLITLIGLAAKNAILIVEFASQKMEEGMPVAEAALEAARLRFRPI
                     VMTSMAFVLGIVPLVIATGAGAAARQSMGTGVFGGMILATFVATIFIPLFFTWLSGKH
                     VRHHGQIETELKSQETV"
BASE COUNT         2022 a         1720 c         1471 g         1922 t
ORIGIN      
        1 tacaaatttc aaaactctac aaagttgcag aaaaacattt tctgcaaaat gaagctatgg
       61 ccatttattt tgacccaacc agtgctgaac aaaaattgac gaccatttca tctatgtaca
      121 ttcaagcttg catgttgggg tcgttagagg ggttaagctt taaatcgcag caaattgaat
      181 tagcgcataa aattcttgat ttttgggcag ctaaagtcag catagaccgt gattacgatt
      241 cacaaaaata ttttttctat gttgatacag gcagtaacac accggccaaa cgtattcgta
      301 aatttaaacc caccgagcat tgccgctact ggtcgcttga cggcatcaac tcaaaagtcg
      361 agctgtgcct ctcattgcta gaatttaata ttgcacctaa acaagaaaag attaaagaaa
      421 ttgtcaacag caaatatgcg ctcatcactt taggcgcact gcaggcagag tggtcacgca
      481 ttgacccttc acgccaacgc agaagccttg agcgtgttaa aactgaaaaa gctgccaaca
      541 ccacttttgg ttttgaagaa acctgcgacc gcatcagaca ttatgaaagt atttcggaac
      601 acaaagttgc tgagcccaag agcgcagaga ttatcttgac tgaaaaagac acaaaccatg
      661 aatctatcaa ggggcgtggt gccaacatta tttatgtgga tttaggtgct gggcaatcga
      721 atattgtgga tgaaagcctg cgtggatttg gcttgctggt acgcaaacaa gccagtgaat
      781 tgagtatcgg catgatgatt ggtgtatcgg ccaaagaaaa aagccatgac attaaagttg
      841 gcgtgattcg taacattaag cctgtggcag gtaaccagtt tcatcttggt attgaattac
      901 tcagccgtgc cgcattttgc gcaaaagctg aaaatgtcag ccgtcagtta attaacacca
      961 cttcggctaa ccagctaaac gacaccattt caggctttag caatgacttt acccgcttta
     1021 cctgcttgtt tttaccaaaa gagttttctg tctctaaaca agaaacgctg attgtgccgc
     1081 gctatcagta caacagaaaa gatgcgttca aaattaatat tgcaggacac gacatgctcg
     1141 tacgcttaac tgacagtttt gaacagcatg aaaactgggt ccgtgtggcc ttcacaaaag
     1201 cgtattaaaa agccatattt tctcccgaga gaaaaacgct gccctgcgag ccttataaat
     1261 aaaggcttgc agagcatctc aaaaggtttg tgtgtttttc aatataaaat tacatgattt
     1321 aacatcggtt caatgcgtgg tgtgtctgca ccaaatagtt aaatcatttg gtgaaattgg
     1381 cttaatattt aacgtgtttt ttgcttggca atatcagcag aagttttagg attattcacc
     1441 tcatattcca gcgaagtgcc cttaaaagtg atggctggct gcacctgatt agctgactca
     1501 gtattctgaa tacgcggatt ttggcgcgct ctcacttgcg ttaatactgc ctttgagcag
     1561 ctattttcaa taaaatcagc acgaataagt tgatgatttt tgctatccaa aaataaacta
     1621 ttagcatcac ccgagtttcg attactgctg gcaatcaatt ggctaccagt aggcagggtc
     1681 gttttatcca cttttaacac atgcgtttgc gcgctcaggc cgcaaaaact aaatttacct
     1741 gcaacatcgg taatcaaata ggtaccgtct tctatgtaaa tacgcacatt gggtatgccc
     1801 aactctttta agtcttgtcg ctgattgtgg ttacaatcca catacacctt gccagccacg
     1861 cagctctcgt tcgccgcgac ttgtacagat gccagtgcgc aaaaaacagc cacccataac
     1921 gcgtgagatt tcatacaaaa tgcttggcgc ttaatgttca ttgggttgtg catctaaatt
     1981 tgtcatctca ttcaattttg ctatgttcat catgttttag cctgcctgaa tagtgtacac
     2041 caaccagcta aaagcaaaac acttgttcaa ctaagcgact aacgataata tgcacgtaac
     2101 acatcgatga tgtaagcgct ttaggaggct ttgtgcaaat tcaaactggc gaaatatacg
     2161 gtaaacaaac ggcggctaaa tttgtcatgt acaacgtgct taaagtcagc aaatcggttg
     2221 ttactctaca aaacattgac aatccgctta gcctgtttga aaccaccgcg caaaaacttc
     2281 tgagttctgg ttatattcgt attagccaaa caccgtatat tgatttagtt tcaacaaccc
     2341 caaaaaaacg taaagctgcc aaaaaaccga cgcgctgccc gctgacttat gatttcttag
     2401 aagcccgtgc agatagtgaa agacctgcac aaatattaac agatttgttt aactaaatat
     2461 tttatcttat tgtttagtaa caacattgtt tgttttgtct ggcgcattcc agccgccacc
     2521 caatgcttta aacagcgcaa cagaggccag caaccttgac tgtcgacttt gaataaaagc
     2581 gattgaagtt tcgttataaa cacgttgggc atccagcaca tctaaatagg cagaatagcc
     2641 agattgataa cgattttcgg caatttcaag cgctttttta gcagattgct ggctcgcatt
     2701 taaggcagtt tcacgtgcgg tatgttgtcg caaatcaacc aaggcatcgt tcacctctct
     2761 aaaagcgttt tgaacagcgc cttcataact tgccaaggct tgcttttgtt tggcgctagc
     2821 ctgatctacg cgcgcgttac gtttgccaga atcaaatatc ggcaaatata aattcaagcc
     2881 gcctgtccaa attctagaag ccgatttcag cacatcagcc agttccatac tttcgccacc
     2941 aaaaattgct gtcaatgaaa ttgtaggata caaagcggcc ttggctacgc caatatttgc
     3001 gtttgccgct accattttcg cttcagcctg tgcaacatcg ggccgcgctt caagcaagct
     3061 cgaaggcaaa cccgcaggtg gcgttggtgg aacgggtaac tgctgaacat ctcccacttg
     3121 tgcaagcgtt aagttcaaat tgccggtcaa gactgccagt tgatggtgac tttgtgcccg
     3181 taatcgcgtt aattctgcca attgtgcact caaaattgca ctggctgctt ccgcctgatg
     3241 cacttctaaa atggaagcaa ccccaccttc taagcgacgt tttgtaagcg ctaaactttc
     3301 atcccatgtt tttaggttcg cttgggtgac gctgatttgt gacgctaagc tacgtagcgt
     3361 taaataattg ctggccacca aacctgtgag tgaaagcgca accgtgtctt ttgcgtattg
     3421 actcgccagc acctgcgcac gtaccgattc tttggcacga atcaacttgc cccaaaaatc
     3481 aatttcaaag cttgtgccta attgcgcccg atagagatta cgcgggttat ttgaaacttc
     3541 attggcaccc gattcagtca cgcgtgaacg gttggccgct ccatttaaat ccacttgtgg
     3601 caatgatgcc gcgccaatct cattggctaa cgcattggct tcttcaatac gcgctacggc
     3661 taatttaata ttgctattgt ttactaatgc ttgaccaacc aaatcattca atatcgggtc
     3721 ttgataatgt gtccaccaat ttgccgctat cgtttggctg acttgactca ccccctcagc
     3781 ataagtcgca ggcagagaaa cttctggacg ctgataatct ggccctaccg ttttacaacc
     3841 cgccatcagc acagcaacca ctgacaagcc aattaggcgc aacttcaacc ttggatgatt
     3901 gttactttga ttgcaaacca tcacaccgtc tcctgcgatt ttaattcggt ttcaatttgg
     3961 ccatgatgcc gtacatgctt gcctgacaac caagtaaaaa agagtggtat aaatatcgtt
     4021 gccacaaagg tggctaaaat catgccgcca aataccccgg tacccattga ttggcgtgcc
     4081 gcagccccgg cacccgtggc aataaccaac ggcacaatac ccaacacaaa agccattgag
     4141 gtcatgacaa tcggcctaaa ccgtaatcgt gcagcttcaa gcgcggcctc tgccactggc
     4201 atgccctctt ccattttttg gctggcaaac tctacaatta aaatcgcgtt tttggcggcc
     4261 aatccaatta aagtgatgag cccaatttga aagtaaatat cattaggcat accgcgcagt
     4321 aataccgcca aaaaagcccc tgccagcgca aatggcacgg ccataatcac tgccaatggc
     4381 aaagcccatg tttcaaactg tgccgctaaa attagaaaca ccatgatgat ggcaaaacca
     4441 aaagcaaaca tggcggctga cccagtacgc ttttcttgat aggcttgggc agtccatgca
     4501 atttgatagc cttcaggcaa gtttgcttcc gcaatccgct caaccgtttg aatggcatca
     4561 cccgaactca cgccactcgc accgctacca aacacttttg cagaaagtaa gccgttataa
     4621 cgctcaagtt gttctgcccc aacaatattg gtcactttac tcaatgcaga gagtggaatc
     4681 atattgccgg caggatttac agcggtaggc tggctacgca catacacttt gcctaaatct
     4741 tcaggctgca tacgatattg ttgttcagct tgcaattgca cacgataagt acggccattt
     4801 cggttaaagt cattcacgta aaatgaaccc atcgtacttt gcaacgtggc ataaatatct
     4861 gagattctta ccttttgtga gagcgctttt gcttcatcta cttcaattaa cagttgcggc
     4921 acggtcggcc gaaaaaaagt attcaaaccc gttaagtttt tatctgccgt caatgcttca
     4981 ataaaacgat tggtcacttc agctaactta attgggtcgg tatctaattt actttgcaca
     5041 tacacttcaa aaccgcctgc tgagcccaaa ccgcgaatag ccggtggatt cactgcaaag
     5101 gcaatgccgt cgggttggct catgccgatg ccaaataatt taccgacaat atcatctgct
     5161 gttgcagtgc ggtcttgcca atctttaagt ctaacaaaca tggtggcagc attggtttta
     5221 ttcgcccctg tcagcaactc taagccgttc acggcaaact catgtgccac ggcatcgtct
     5281 tgatgaatcg ctgcgcgaat attttctgtg gtttttgtgg tgcggctaag cgttgcgcca
     5341 ttgggcatca ctacaattgt caccacgtaa ccttggtctt cggctggcac aaaactgcca
     5401 ggcaccttca tgactaacaa aatcacggtt gcaacaatac ccaaaaacgc aatggcacca
     5461 atgattttat gtttaagcgt gacaccaacg gtatgcgtat aaaaatgtgt gaacctcgta
     5521 aacgattggt taaatttagt aaaaaaccaa ttttctccat gggatttttt taaaatcatg
     5581 gcacaaaggg ctggcgttaa agtaagcgcc acaatgcctg aaatgactac tgaaatggct
     5641 accgtaactg caaactgacg gtataactcg cctgcaatac cgccctgaaa agcaacaggc
     5701 acaaacaccg cgcacaatac caatacaatc gccaccacag cggctgatac ttggtgtatg
     5761 gatttaatcg ctgcgggcag cggtgctaaa ttttcttcac gcatcaatcg ctcagtgttt
     5821 tccagcacca caatggcatc atccaccaca atgccaatgg ctaaaatcat ggcaaacagc
     5881 gtgagtaagt taatggaaaa accaaacata tagaggccag caaaggtgcc aattaaagaa
     5941 ataggcacgg caataattgg tattaacgtt gcacgccagc tctgtaaaaa taaaaatacc
     6001 accagcacga ccagcaccaa tgcttcacca aacgttttta gtacctcatt gatggtggct
     6061 ttcacaaagt cactggtgtc ataaggaatg gtgtaagtca tgccttcagg aaaacgtgct
     6121 tttagttctt ctactttatc tttaatgcct tttgcggtat ctagcgcgtt cgcgccactt
     6181 tgtaaaaata ttggtagcgc aacacctgtt tgcccattca aggctgaagt cacattgtag
     6241 ttttgcgccc caagttcaat tcttgccaca tcttttaaat acaaaacacc gcgcgggcca
     6301 tccgctttaa tcatgatgtt agcaaattgc tcagggtcaa ccaatctgcc ttttgccgtg
     6361 acggtgtaga ccaactgctg gcttgcaggc gcaggctcag caccaatctt gccggcagca
     6421 tactgattat tttgtgcgct aatcgctgcg gcaatatcac tggtagtgac acctaattgc
     6481 gccatacggt ctggccgcaa ccaaatacgc atggagtaat cttgcccacc aaaaatcagc
     6541 gcgtccccca cccctttcac gcgcttaaac tcatccaata cattgagcgt tgcgtaatta
     6601 cttaaaaaca gccggtcatg ctttgggtct tttgacgtca gcatcacgac cagcaaaatg
     6661 tcattcgatt ttttttgcac aatcaggcca ttacggcgca catcatcggg caggcgtggc
     6721 gtagcaatat ttacgcggtt gctaacgtta aacgttgcct ggtcaacatc tgtaccaact
     6781 tcaaacgtag cggtaatcac caatgtgccg ctagagtctg cactggatga aaaataaagc
     6841 aggttatcca cgccactaag ctgctcttca atgggtgctg caacggtttt tgacagtgtt
     6901 tctgcactgg ctcctgggta tgaggcagtg atcgtgaccg taggcggagc aatttgcgga
     6961 tactgcgcaa taggtaactt aaacgccgcc gccagccctg ccagcacaat aataatggac
     7021 agcacggagg caaagatggg gcgcgtgata aaaaacttgg tcatgtcttt tatcctttat
     7081 ctgctggctg attttggctt gcaggacttg attcagacgt agcccctact atatt
//
LOCUS       DAOQSI010000112         7103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_10339_length_7103_cov_6.080874, whole genome shotgun sequence.
ACCESSION   DAOQSI010000112 DAOQSI010000000
VERSION     DAOQSI010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7103
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10339_length_7103_cov_6.080874"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PKL42_07560"
     CDS             <1..327
                     /locus_tag="PKL42_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNU66970.1"
                     /translation="RDNGQALPRITPLRLGAGLRYQKNALGARVDVLHAFSQNRTAEN
                     ELKTDGYTDVSATVTYKLPTKVHVELFAKAKNLLNQEIREHTSFLKDISPAGERSVLL
                     GLRADF"
     gene            complement(366..734)
                     /locus_tag="PKL42_07565"
     CDS             complement(366..734)
                     /locus_tag="PKL42_07565"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HNU66971.1"
                     /translation="MESPFKGKTGLSRLINAFGYSVEGFKAAFKHEDAFRQEVFLSVV
                     LIPLGLYLGKTGVEKALLVSAVLLVLIVELFNSAIEAAVDHTSLERHALAKRAKDIGS
                     AAVFVALGIVVSVWGLVLFL"
     gene            843..1115
                     /locus_tag="PKL42_07570"
     CDS             843..1115
                     /locus_tag="PKL42_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HNU66972.1"
                     /translation="MARTVKCIKLDKEAEGLDFPPYPGALGKRIFENVSKEAWAAWLK
                     QQTMLVNENRLSLADPSARKYLTEQTEKYFFGEGADTASGYVPPSK"
     gene            complement(1373..2032)
                     /gene="rpiA"
                     /locus_tag="PKL42_07575"
     CDS             complement(1373..2032)
                     /gene="rpiA"
                     /locus_tag="PKL42_07575"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HNU66973.1"
                     /translation="MAYTQDELKQQVARAAVEYVKEGIIGVGTGSTANFFIEELAKVK
                     HKIEGAVASSEATAQRLRAHGIEVFDLNNVDSLDIYVDGADEITEHLHMLKGGGGALT
                     REKIVAAVAKTFICICDETKYVPVLGKFPLPVEVLPMARSYVARELVKLGGQPSLRNF
                     TTDNGNVILDVHGLNIVDPIATEAKINQIVGVVTNGLFAARPANVLLLATAEGVKTYK
                     K"
     gene            2115..3638
                     /gene="ilvA"
                     /locus_tag="PKL42_07580"
     CDS             2115..3638
                     /gene="ilvA"
                     /locus_tag="PKL42_07580"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HNU66974.1"
                     /translation="MTTIAPINYQEKIEHSRVYDVANKTPLEFQPNISARIGNRVLLK
                     REDMQPVFSFKIRGAYNKMAHLPKDVLKRGVIAASAGNHAQGVALSAQKLGCRAVIVM
                     PTTTPAIKVDAVKNRGAEVVLFGDSYSDAYVHALELEKAEKLTFVHPYDDPDVIAGQG
                     TIALEILDEHPQPIEAIFCCVGGGGLLAGIAAYVKAVRPEIKVIGVEAKDAEAMTESL
                     KKGERIILDQVGLFADGAAVKQVGVHTFKLCQQYVDEMIVVDNDAICAAIKDVFEDTR
                     SILEPAGALALAGLKEYAERENLHNKTLIGIASGANMNFDRLRFVAERAEIGEKREAV
                     LAVTIPEKPGAFKNFCRLLGNRNITEFNYRYSDPKNAHIFVGIAITDPTESAKLVADL
                     QAQGLSTLDLTDNEMAKLHLRHLVGGHAPQAEHEVVYRFEFPEKPGALMKFLDTMGHD
                     WNISLFHYRNHGADFGRVLVGMQVPPNEQDEYLNFLHNLGYPYWDETNNPAYQLFLG"
     gene            3838..4995
                     /locus_tag="PKL42_07585"
     CDS             3838..4995
                     /locus_tag="PKL42_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66975.1"
                     /translation="MKYTQHKTRLSLVLLALIAGLSACTKEKSEEAKTSSDSATNNQA
                     ADTTIPTNSVTASGFAYVTSQDAGVSVIDLASMKVIKQFDVKAESPRGIGITDDGKKL
                     IVATRGNDSISVIDTATGEVLQQILIGKNPEFVRVRGNFAFISSEPSAKGGPPPKPGA
                     KEEEDDDDDEEKIPAQIVVVDLAQGKKVREITGGPETEGIEFSNDGSKMIITNEADNT
                     VTVHNIETGELLKTISTHEHGDRPRGIKASPDGSNYIATLEYGNKFMVLDKDFNFVRT
                     VDTGETPYGVAYDATGNNIYVASNKAKLLEVFDAKTFVKTKEVTTGNRCWHFSFTPDH
                     QQILLACGKSDAVLVVDANQFTVSSQIEVKGMPWGVVTYPKAMGSLETSIR"
     gene            complement(5081..5542)
                     /locus_tag="PKL42_07590"
     CDS             complement(5081..5542)
                     /locus_tag="PKL42_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HNU66976.1"
                     /translation="MRYWLMKSEPSDVSIDDLAAMPNQTVDWYGVRNYQARNFMRDQM
                     QLGDGVLFYHSNCETPGIAGICEVSQLAYPDRLQFIKGHHYYDEKSTPENPRWLNVDV
                     KLVQKTRLLSLKEMRETPELANLRILQRGNRLSITPVDPKDWQFIMDILKR"
     gene            complement(5834..6139)
                     /locus_tag="PKL42_07595"
     CDS             complement(5834..6139)
                     /locus_tag="PKL42_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNU66977.1"
                     /translation="MSENKGVDVNIMGREFTVACSEEERPGLIEAVNFLDKKMRDIRD
                     TGKIVGVERIAIMAALNLAHELLNTKNGTVDVGDIKRRISHMQDQIDQVYATKELFS"
     gene            complement(6153..6341)
                     /locus_tag="PKL42_07600"
     CDS             complement(6153..6341)
                     /locus_tag="PKL42_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66978.1"
                     /translation="MDADLKALETKLTGLISLCTDLRNQNAQLRQDLDATLRDTAELK
                     ANMAQASERIEALMETLP"
     gene            complement(6475..7065)
                     /gene="cobC"
                     /locus_tag="PKL42_07605"
     CDS             complement(6475..7065)
                     /gene="cobC"
                     /locus_tag="PKL42_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ribazole phosphatase"
                     /protein_id="HNU66979.1"
                     /translation="MKLTLVRHTTLDIAPHICYGQSDVDVSANFDNERLALQRKLGDT
                     QFDAIYTSPLRRCQQLARALCHHNNFGFTADDILHDHRLKELNFGDWEMQAWDVIPRD
                     MFDVWANDYGNLAPPNGETFSQLHARAKDFVDEVAKDAQNQHLLVITHGGFIRALIAE
                     VLQMPLKRLFRLSISHASVTLLEFKDDVPKVHFMNL"
BASE COUNT         2072 a         1600 c         1507 g         1924 t
ORIGIN      
        1 cgagataacg gccaagctct gccgcgcata acgccgctac gtttgggcgc tggcttgcgg
       61 tatcaaaaaa atgcactggg tgccagagtt gatgttttac atgcatttag ccaaaaccgc
      121 acggctgaaa atgaacttaa aacagatggt tataccgatg tgagcgcaac agtgacttat
      181 aaattaccca ctaaagtgca tgttgagcta tttgcaaaag caaaaaatct gctcaatcaa
      241 gaaattcgtg aacatacgtc tttcttgaaa gatatttcac ctgcaggaga acgctcggtc
      301 ttgctggggc tgcgtgccga tttttaaata agccacatat tgtaggagcg attttcaaat
      361 cgctcctaca aaaacaacac caaaccccaa acgcttacca caatacccaa cgccacaaaa
      421 acagccgcac tgcctatgtc ttttgcacgt ttagccagtg cgtgacgctc caaagacgtg
      481 tgatccaccg ctgcttcaat cgccgaatta aacaactcca caatcagcac cagtaacacg
      541 gcagatacca acaaagcctt ttcaacccct gttttaccca aataaagccc aagcggaatc
      601 aacacaaccg acaaaaatac ttcttgccga aacgcgtctt catgtttaaa cgctgcttta
      661 aatccttcta ctgaataacc aaacgcatta atcaaacggc ttaaccccgt tttaccttta
      721 aatggacttt ccatgacacg ccttcattaa aaaactaatt tgcgaatgat aacatgcgca
      781 ctcgcgccgt ttaaaggctt gcgctatcat agtggtttta ttgaacacct aaaggcacaa
      841 caatggcacg caccgtaaaa tgtatcaaac tcgacaaaga agcagaaggt ttagattttc
      901 ctccctaccc tggcgcgttg ggcaaacgta tttttgaaaa tgtttcaaaa gaagcctggg
      961 cagcttggtt aaaacagcaa accatgctgg tgaacgaaaa ccgtttaagc ttggccgacc
     1021 cttctgcgcg taaatatttg accgaacaaa ctgagaagta tttttttggt gaaggtgcag
     1081 atacggcgag tgggtatgtg ccaccgagca aataagtgat atgcaaaata tggtttaatg
     1141 aaattctatg ttatttcata tttcattcgg atttgtctac ataatccacc atatgaaaag
     1201 ttttaatttt taacgccaaa tcaacacaac ccttttcacc tcctccgaaa gccttgtaaa
     1261 taaaggctcg cagggcagcg aaaatctctc gagggatttt tagtttgttt aggcaattaa
     1321 aaaggtgcgt aactaccaag ttacgcacct taaattttaa gcagctacag atttattttt
     1381 tgtaagtttt tacaccctca gcggttgcca ataatagtac attggctggg cgcgctgcaa
     1441 ataatccatt ggtgacgacg ccaacgattt ggttgatttt tgcctcagtc gcaattgggt
     1501 caacaatatt taaaccgtgt acgtctaaaa tcacgttgcc gttatcggtg gtaaagttac
     1561 gtagtgatgg ttggccgcca agcttgacca actcacgcgc aacgtaactg cgcgccattg
     1621 gtagcacttc tactggcaac ggaaatttgc ctaataccgg cacatatttg gtttcatcac
     1681 agatgcaaat aaaggttttt gctacggctg ctacgatttt ttcacgggtg agtgcgccgc
     1741 cgccgccttt gagcatatgc agatgctcgg taatttcatc tgcgccatct acataaatat
     1801 ctaggctatc gacgttattt aaatcaaaca cttcaatacc atgcgcacgt aaacgttgcg
     1861 cggtagcttc tgagctggcc actgcacctt ctattttgtg ttttactttt gcaagctctt
     1921 caataaagaa gttggcggtt gagcctgtgc ccacgccgat gatgccttct tttacgtatt
     1981 caaccgcggc gcgtgccact tgttgtttta attcgtcttg ggtatatgcc attttgcctt
     2041 tttcctctac aaattctcta taatgtaagt caattcaaca taatacactg ctgacgcatt
     2101 tttaagaata tcgcatgaca acaatcgcac ccatcaatta tcaggaaaaa atagagcatt
     2161 cgcgcgttta cgacgttgcg aacaagacgc cgcttgaatt tcagcctaat atttctgccc
     2221 gtattggcaa ccgtgtgttg ctcaagcgcg aagatatgca accggtgttt tcattcaaaa
     2281 ttcgcggtgc atataacaaa atggcccatc tgccaaaaga tgtgctcaaa cgtggcgtga
     2341 ttgcagcctc tgctggcaac catgcgcagg gtgtggcatt aagcgcacaa aagttaggct
     2401 gtcgcgcggt aattgtaatg ccaaccacca cgccggccat taaagtagat gcggttaaaa
     2461 accgtggtgc tgaagtcgtg ttgtttggcg acagctattc agatgcctac gtacacgcgc
     2521 ttgaacttga aaaagccgaa aaactcactt ttgtgcatcc ctatgacgac cccgacgtga
     2581 ttgcagggca aggcacgatt gcactcgaga ttttagacga acacccgcaa cccattgaag
     2641 ccatattttg ctgtgttggc ggcggcggct tattagcagg cattgcggcg tatgttaaag
     2701 cggttcgccc agaaatcaaa gtcattggcg tagaagccaa agatgccgaa gcgatgacgg
     2761 aatcgctaaa aaaaggcgag cgcatcatcc ttgaccaagt agggcttttt gccgatggtg
     2821 ctgctgttaa acaggtgggc gttcacactt ttaaactttg ccagcagtat gtggatgaaa
     2881 tgattgtggt cgataacgat gcgatttgtg cagccattaa agatgtgttt gaagatacgc
     2941 gcagcattct tgagcctgct ggcgcgctcg ccttggcggg gcttaaagag tatgcagaac
     3001 gcgaaaacct gcacaacaaa acgctcattg gtattgcatc tggtgccaac atgaactttg
     3061 accgcttgcg atttgtggcc gagcgcgccg agattggcga aaaacgtgaa gcggttttag
     3121 ctgtgacgat tcctgaaaag cccggtgcgt ttaagaactt ttgccgatta ctgggtaacc
     3181 gcaatattac cgaattcaac tatcgctatt ctgaccccaa aaatgcccat atttttgtag
     3241 gtattgccat tacagaccca acagaatctg ccaagttggt ggcagattta caagcacaag
     3301 ggttatctac gttggattta actgataacg aaatggctaa attacatttg cgccacttag
     3361 tcggtggtca cgccccgcaa gctgagcatg aagtagtgta tcgctttgag ttccctgaaa
     3421 agccaggcgc tctcatgaag tttttagata cgatggggca cgactggaac atcagtttat
     3481 tccattaccg caatcatggc gcggattttg ggcgcgtgct ggtgggtatg caagtgccac
     3541 caaatgagca agatgaatat ctcaattttt tacacaactt agggtatcca tactgggatg
     3601 aaacaaataa ccccgcttat caattatttt taggatgagt gtcattatag attggctgga
     3661 taaaaccacc atttattggt tatattcagc caataaatgg tgcattaaat tacaatgcat
     3721 tgtttaaatt caagtttatt ttcagcacgt ttcttgcttg gaaatacccc ttaaggcatt
     3781 gaactaaatc aataaaaatt gaccttacta ctgtagcaat cactttggag atattaaatg
     3841 aaatatacgc aacataaaac gcgcctgagt ttagtgcttt tagcgcttat tgcagggtta
     3901 agcgcctgta ctaaagagaa atctgaagaa gcaaaaacga gtagtgactc agctacaaat
     3961 aaccaagcag ccgacactac aataccaacg aatagtgtaa ctgccagcgg ttttgcttat
     4021 gtaacttcac aggatgcagg cgtgagcgtg attgacctcg cctcaatgaa agtgattaag
     4081 caatttgacg taaaagctga aagcccacgt ggtattggca ttactgatga tggtaaaaaa
     4141 ctgattgtgg caacgcgtgg caatgacagt atttcagtga ttgacaccgc gactggcgaa
     4201 gtattacagc aaatcttaat cggcaaaaac cccgagtttg tgcgcgtgcg cggcaacttt
     4261 gcttttattt cttctgaacc aagtgctaaa ggtgggcctc caccgaagcc aggcgccaag
     4321 gaagaggaag atgacgacga tgacgaagag aaaatcccag cacaaattgt ggtggttgat
     4381 ttagcgcaag gtaaaaaagt acgtgaaatc acaggcggcc cagaaactga aggcattgag
     4441 ttttcaaacg atggtagcaa gatgattatc accaatgaag ccgataacac ggtgactgta
     4501 cataacattg aaacaggtga gttacttaaa accatttcca cacacgaaca cggcgacagg
     4561 ccacgcggca ttaaagcatc gcccgatggc agcaactaca ttgcaacatt agaatacggc
     4621 aacaaattca tggtattaga taaagacttt aactttgtac gcactgttga tacgggtgaa
     4681 acaccctacg gcgttgcata cgatgcgaca ggtaacaata tctatgtggc aagtaacaaa
     4741 gccaagttgc ttgaagtgtt tgatgcaaaa acgtttgtta aaacgaaaga agtcaccaca
     4801 ggcaatcgct gctggcactt tagttttaca ccagaccatc agcaaatctt gttagcctgt
     4861 ggtaagtcag atgccgtgtt ggtggtagat gccaatcaat tcaccgtaag cagtcaaatt
     4921 gaagtaaaag gcatgccttg gggtgtggtt acttacccta aagccatggg cagtttagag
     4981 acttcaatca gatagaactc ttggtatatt gcagcacata aaaaagcccg agcagtcggg
     5041 ctttttttta tttagtcacg gttgaaatca ccgtttaaaa ctaacgcttt aatatgtcca
     5101 taataaactg ccaatctttt gggtcaactg gcgtaataga tagacggtta ccgcgctgca
     5161 aaatacgcaa attggcaagt tcaggcgttt cacgcatttc tttcaagctt aaaagccgcg
     5221 ttttttgcac caacttcaca tcaacattca accagcgtgg attttctggg gtagattttt
     5281 catcgtagta atgatggcct ttgatgaact gcaaccggtc aggataagcg agctggctca
     5341 cctcgcatat gcctgcgatg cctggtgttt cacaattgga gtggtaaaac agcacgccgt
     5401 cacccaattg catttggtca cgcataaaat tgcgcgcctg ataattgcgc acaccatacc
     5461 aatcaaccgt ttgattaggc atcgccgcta aatcatcaat agaaacgtcc gagggctcag
     5521 acttcatcag ccaataacgc attttttagt cttaaattgg atttattatc attttaatga
     5581 gggaagttct ctacggatat ggcctagacc agcatcctga accaaaaagg ctcaagtggt
     5641 aacagcctag agcgcattag gtacaccaac catgtggcgc gcacaccgca actcgaatga
     5701 ctaaaaccga tgctataaac tagttcaagg aattattagc ctaaaccgca ccgcagagaa
     5761 caaactttga aactgaaaac gctcactaca attctacgcg caataacgga gtttgtatta
     5821 acgtgaagtt gaattaacta aaaagctctt ttgtggcata aacctgatca atttggtctt
     5881 gcatgtggct gattctgcgt ttaatgtctc ccacatccac tgtgccattt tttgtattga
     5941 gtaattcgtg tgccaaatta agtgctgcca taatggcaat ccgctcaacc ccaacaattt
     6001 tacccgtgtc gcgaatatca cgcatttttt tatctaaaaa attaaccgcc tcaatcaagc
     6061 cggggcgttc ttcttcagaa catgccaccg tgaattcacg gcccattatg ttgacatcta
     6121 cacctttatt ttcgctcatc cgtttttacc cgctatggca aggtctccat caacgcttct
     6181 atacgctcac tggcttgcgc catatttgct tttagctctg cggtatctcg caaggtggcg
     6241 tctaaatctt ggcgtaactg cgcattttga ttgcgcaggt ccgtacacag ggaaattaaa
     6301 cctgttaact tagtctctaa tgcttttaaa tcggcgtcca ttcagtcact ataagttaaa
     6361 aagtttattg tagtcaatag ctaacgtgta tttttaaatg tattacaaca cctattagtc
     6421 ttaacgggtg aatgtgcgtt atttttacga gacatcgtct taggttaagt ttgactataa
     6481 attcataaag tgcacttttg gcacatcatc tttaaactca agtaaggtga cgcttgcatg
     6541 gctaatagac agtctaaaca aacgtttgag tggcatttgt aaaacttctg caattaacgc
     6601 acgaataaat ccaccatggg taatcactaa tagatgctgg ttttgtgcgt cttttgctac
     6661 ttcatctaca aaatctttag cacgcgcatg aagttgtgaa aatgtttcgc cattgggtgg
     6721 cgctaaattg ccataatcat tggcccatac atcaaacata tcgcgcggaa taacatccca
     6781 ggcttgcatt tcccagtcac caaaatttaa ctctttaagg cggtgatcat gcagaatatc
     6841 gtctgctgta aacccaaaat tattgtgatg acataaggcc cgtgctaact gctggcaacg
     6901 ccgcagcggg cttgtgtaga tggcatcaaa ctgcgtatct ccaagtttgc gctgtaacgc
     6961 taatcgctca ttgtcaaaat ttgctgaaac gtcaacatcg ctctgcccgt aacaaatatg
     7021 cggtgctata tctaaagtag tgtggcgcac caacgtaagc ttcatacgaa cctaaccaat
     7081 acacttaaac ttgcaagcaa acc
//
LOCUS       DAOQSI010000113         7065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_10423_length_7065_cov_5.190870, whole genome shotgun sequence.
ACCESSION   DAOQSI010000113 DAOQSI010000000
VERSION     DAOQSI010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 7065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7065
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10423_length_7065_cov_5.190870"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      258..480
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            613..2475
                     /locus_tag="PKL42_07610"
     CDS             613..2475
                     /locus_tag="PKL42_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNU66980.1"
                     /translation="MKKTIIASLIGAAFTTPIYAADAVNFNDIIVTASRTPQTREAPV
                     ADVSVIDVEEIQRAGQSTLVEILARQPGIEITSSGGAGTISSIFMRGTNSGHVVILID
                     GLRIDSATLGSVALENLPVAQIQSIEILRGPASSLYGQDAIGGVIQIFTKKGSGDIKF
                     NANLGYGRYNTKRADAGVSGGIGDTRFALNVSSLNTHSFSSIDTQNSNYNDDDANRNL
                     AFSGNVSHQIENGHEVGVQFFHTDGRVHFDNRFNGSNFDSRSDSKQYSFALFSNNQFT
                     DVWLSKARIGQGADESVTFDEFSTAGGDLYKTKQLQLSWQNDFKLPVGTLTLLYDGLF
                     QKVRSNSVSYSTKARDNDGFVASYLLDYDAHSLQLSARHDHNSQFGRHNTSNIGYGYK
                     LNQNWRLTAGYGTAFKAPAFNELYWPYTDYGFGFSYGGNPNLAPEKSRNFETSARFQD
                     EDTTATITAFHNRIDNLIGLNNLAAAQPVNINQATINGLSVTANQRLGNFNLSGSVDF
                     QSPKDDKTDKLLVRRANRHASANVSYTWGDWRIGAESIASSKRYNDPANTKSMSGYAL
                     LNATVDYKLSQDWSIQARANNILDKKYALGLDFGGEAYNTPRANLFVNIRYQPE"
     gene            2597..3175
                     /locus_tag="PKL42_07615"
     CDS             2597..3175
                     /locus_tag="PKL42_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU66981.1"
                     /translation="MEQTNSPSYVKPIVIASAITFIMLATRGSHVLTNVSLPDASLAL
                     FLVGGLLLRRAAWFVFFVVLATVIDFSAAAVDSWFGFCLTAGYWGMLPTYAVMWLAGV
                     WLAKQSDSLSVPKFTLAALLATFAAFVISTQTYYLFSGRFPNTGLLETLQYGWNYLPS
                     YMSYTAAYLLAFWVSAKTLPKLEQLINSVKTA"
     gene            3259..3861
                     /gene="cobO"
                     /locus_tag="PKL42_07620"
     CDS             3259..3861
                     /gene="cobO"
                     /locus_tag="PKL42_07620"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNU66982.1"
                     /translation="MTVDKNARHASRMARKKAVIDSAIEQANQEKGLLLVLTGNGKGK
                     SSSAFGMAARALGYGMKVGVAQFIKGRSDTGEEAFFSALPNVEWHVLGEGFTWDTQDL
                     ERDIHTAKAGWEIAKKMLQNPEVNLVILDELTYTLNYGYLETSEILADIAARPPMQHV
                     VVTGRAAPQVLIDLADTVSEITDVKHAYRAGIKAQPGIDL"
     gene            3877..4404
                     /gene="cobU"
                     /locus_tag="PKL42_07625"
     CDS             3877..4404
                     /gene="cobU"
                     /locus_tag="PKL42_07625"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HNU66983.1"
                     /translation="MPIHFILGGARSGKSAFAEKLAFESQLSVTYIATAQVYDAEFGK
                     RIAHHQARRPAHWQTIEQPFQLGQTLQTHTKEDVCIVVDCLTLWLAQCIFPDCDRPAD
                     LNWQDERQAMLDVLPTLKGNIILVSNEVGMGIVPLGEINRQFQDEQGRLNQAVAQIAH
                     QVSFIAAGLPIVLKG"
     gene            4407..4625
                     /locus_tag="PKL42_07630"
     CDS             4407..4625
                     /locus_tag="PKL42_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNU66984.1"
                     /translation="METTTFELRGEFIELCQLLKLVGIANSGGQGKMMVADGIVKVDS
                     NVELRKTAKIRAGQLVECFGQQIKVEKP"
     gene            complement(4807..6645)
                     /gene="recQ"
                     /locus_tag="PKL42_07635"
     CDS             complement(4807..6645)
                     /gene="recQ"
                     /locus_tag="PKL42_07635"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HNU66985.1"
                     /translation="MSNTAQQLLHDVFGYSSFRGEQEAIVAHVSAGGDALVLMPTGGG
                     KSLCYQLPALLREGVAVVVSPLIALMQDQVDALKQLGVRAAFLNSSQEANESREVTAQ
                     LMRGHLQIVYVAPERLLMESFLALLEQIEQGASIALFAIDEAHCVSQWGHDFRPEYRK
                     LTVLHERFPQVPRIALTATADAPTRAEIVERLKLEQARQFVSSFDRPNIRYRITQKDV
                     TQKTSARQQLETFLEHEHANDAGIVYCLSRRKVEETAEWLKSRGWDALPYHAGLDASV
                     RNKNQRRFLREEGVIMVATVAFGMGIDKPNVRFVAHLDLPKSMEGYYQETGRAGRDGL
                     PANAWMAYGLGDVVSMRKMLDSGDAPEERKQVERQKLDALLGFCESTQCRHQTLLRYF
                     GEQHPGDCGQCDNCLNPVDTWDATQAAQMALSCVYRTGQRFGVVHLIDVLLGKTNVKV
                     SQFSHQNLSVFGVGKAISQAQWSSVFRQLVAGGYLMSDIEAYGGLKLTESARPVLKGE
                     AEVWLRRDVEVVSRKSSKAERGARAKEGYAEVSDDPLWHALKAKRMELAKEQGVPPYV
                     IFHDSTLLEILNRKPASLTEMSQITGVGQAKLQRYGDAFLAVLEDR"
     gene            complement(6721..6796)
                     /locus_tag="PKL42_07640"
     tRNA            complement(6721..6796)
                     /locus_tag="PKL42_07640"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6761..6763),aa:Lys,seq:ctt)
     gene            complement(6889..7059)
                     /locus_tag="PKL42_07645"
     CDS             complement(6889..7059)
                     /locus_tag="PKL42_07645"
                     /inference="COORDINATES: protein motif:HMM:NF021526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2256 domain-containing protein"
                     /protein_id="HNU66986.1"
                     /translation="MLKKANFPTKLCLACHRPFVWRKKWKAVWQEVKYCSEACRKFKK
                     TKTLLSDLKSIK"
BASE COUNT         1930 a         1710 c         1563 g         1862 t
ORIGIN      
        1 gccaatttta cgaaggccac caagacaaag cgctacggct tgataaaatt agcttttggg
       61 gaattcttgc cacctatatt gcgctcaatt taatactggt catcaaggcc attcactagc
      121 aacaaaacac gacccaactc ccccgagaaa aaataaatgc tctggaagcc tttatttata
      181 aggcttccag agcatcgaaa aaggttgatg tgaaatcttg gctaaaaaat gcctttttta
      241 tttcaaatta acgctataat tacggcttgt tgtcaggtgt caaatttgcg ctctagcaaa
      301 tttggtgaaa ctgggaaata ggtgcgtttt tatctcgata taaaaattag tcctatgctg
      361 cccccgcaac ggtaagtgat ttaacgcttt gcaatttgcc actgaatttt ttgataattt
      421 gggaaggcgc aaagaatatc gtcacaagtc cggagaccgg cctcgcaact taaagagttg
      481 aaaataccgc ggggtgacgg tgttcaggcg cggcggtttc attcgctacc gttttgttca
      541 tgtttgttcc ctgaagtgtt ttctattgat gaatctcgta tgcggggacg catgcgcaat
      601 caggaaaata acatgaaaaa aaccatcatt gccagcctaa ttggtgcggc ttttacaaca
      661 cccatttatg cagcagatgc cgttaatttt aacgacatca ttgtcactgc ctcccgcaca
      721 ccacaaaccc gcgaggcgcc tgtagcggac gttagcgtga ttgatgttga agaaattcaa
      781 cgtgccggac aatccacatt agtcgagata ctcgcccgcc aacctggtat tgaaattacc
      841 agtagtggcg gtgcaggaac catttccagc atttttatgc gcggaactaa ctctggtcat
      901 gtggtaattt taattgatgg gctgcgaatt gattcagcca cattggggtc ggttgcgctt
      961 gaaaacttgc ctgttgcgca aattcaaagc attgaaattt tacgcggccc cgcatctagc
     1021 ctttatggac aagatgcgat tggcggcgtc attcaaatat tcacaaaaaa aggctcaggc
     1081 gatatcaagt tcaatgccaa tctgggctat ggtcgctata acaccaaaag agctgatgca
     1141 ggcgtcagcg gcggcattgg cgatacccgc tttgcattaa atgtatcaag ccttaatacc
     1201 catagtttct catccatcga cactcaaaat agtaactaca atgacgatga tgcaaatcgc
     1261 aatttagcgt tttctggcaa tgtttcgcac caaattgaaa atgggcatga agtaggggtt
     1321 caattttttc ataccgatgg acgtgtgcac tttgataacc gattcaacgg atcaaacttt
     1381 gatagtcgct cagattcaaa gcaatacagc tttgccctat ttagtaataa ccagttcacc
     1441 gatgtctggc tatcaaaagc ccgtattggg caaggcgcgg atgaaagcgt cacttttgat
     1501 gagtttagta ctgccggtgg agatctttac aaaaccaagc agctgcagtt gagctggcaa
     1561 aatgacttta agttgccagt tggcacactc actttgcttt atgatggttt gtttcaaaag
     1621 gtgcgttcaa attcagtgtc gtattcgaca aaagcgcgcg acaacgatgg ctttgttgca
     1681 agttatttgc tggattacga cgcgcattcg ctacagttaa gcgcacgtca tgatcataac
     1741 tctcagtttg gtcgccacaa cactagcaat attggctacg gctataaact taaccaaaac
     1801 tggcgtctta cagctggata tggcacggct tttaaagccc cagcttttaa tgagttatat
     1861 tggccatata ctgattacgg ttttggtttt tcttatggtg gcaacccaaa tctagcccct
     1921 gaaaaatcgc gtaattttga gaccagtgca cgttttcaag atgaagacac cacagccacc
     1981 attactgctt ttcataaccg tattgataat ctcattgggc ttaataactt ggctgcagcg
     2041 caaccagtaa atattaatca agcaacaatt aacgggttaa gtgtaaccgc gaatcaacgt
     2101 ttgggtaact ttaacctgag tggcagcgta gactttcaat cgcctaaaga tgacaaaaca
     2161 gataagcttt tagtcaggcg tgctaatcgt catgccagtg ccaacgttag ttacacttgg
     2221 ggagattggc gtattggtgc tgaaagcatc gcttcttcaa aacgttataa tgacccagcc
     2281 aacaccaaat caatgagcgg gtatgcgtta ttgaatgcca ccgttgatta caaattgagt
     2341 caagattggt ccattcaagc gcgcgcgaat aatattttag ataagaaata cgccctaggt
     2401 ttagattttg gcggcgaagc ttataacacg ccaagggcga atttatttgt caacattcgt
     2461 tatcagccag agtaatctta agcgtacaaa aatgttatga tgcagtcttt taagaaacat
     2521 gaatcgccaa gtttagaaaa agctattagt taagtttaga caacgctatt tacaagtgtt
     2581 agcagtggag aaaaaaatgg aacaaaccaa cagcccatct tatgttaagc ctattgtgat
     2641 tgcctcggcg ataactttta tcatgctggc cacgcgaggt agccatgtgc tgaccaatgt
     2701 atctttacct gatgcaagtt tagctttatt tttggtcggt ggtttattgt taagaagagc
     2761 tgcttggttt gttttcttcg tggtgctcgc cacagtcatt gactttagcg cagcggctgt
     2821 agatagctgg tttggtttct gcctaaccgc aggttattgg ggcatgttgc caacttacgc
     2881 ggtaatgtgg ctggctggtg tatggctagc taaacaatct gatagcctta gcgtgccaaa
     2941 attcacgcta gccgctttgc tggcaacctt tgccgcattt gtcatttcca cgcaaacata
     3001 ttatttgttt tcaggccgct ttccaaacac cgggttacta gaaacgcttc aatatggctg
     3061 gaactatctg ccaagttaca tgagctacac ggccgcgtat ttactggcat tttgggtttc
     3121 ggctaaaaca ttaccgaaat tagagcagct tatcaattcg gtaaaaactg cataattatt
     3181 tctacgctta gctagcattc aagccgcgtt agtgaacaat actaacgcgg ttttttaatc
     3241 gcatttcagg taaatgacat gacagttgat aaaaatgcgc gccacgcaag ccgtatggcg
     3301 cgtaaaaagg cggtaataga ctccgccatt gaacaggcaa atcaagaaaa aggcttgtta
     3361 ttggtgctga ctggcaacgg caaaggcaaa agctcgtcag cgtttggcat ggctgcacgt
     3421 gcccttggct atgggatgaa agtaggcgtc gctcaattta ttaaaggccg tagcgatacc
     3481 ggcgaggaag catttttttc agccttgcct aatgtggaat ggcatgtgct gggcgaaggc
     3541 tttacgtggg acacgcaaga ccttgaacgc gacatccaca ccgcaaaagc tggctgggaa
     3601 atcgccaaaa aaatgttgca gaaccctgaa gtcaatttag tgattcttga tgaactcacc
     3661 tacaccttaa actacggcta tttagagact agcgaaatcc tcgctgacat tgccgcccgc
     3721 ccaccaatgc agcacgtggt ggtaacaggt cgcgcagcac cgcaagtctt aattgatctg
     3781 gcggataccg tcagcgaaat cacggatgtg aagcacgctt accgcgctgg cattaaggca
     3841 cagccgggca tagacttata aaccaaacta ctgcacatgc ccattcattt tattctcggc
     3901 ggcgcccgct ctggcaaaag tgcctttgca gaaaaacttg catttgaaag tcaattatcc
     3961 gtaacttata ttgcaactgc gcaagtctac gatgcagaat ttggcaagcg cattgcgcat
     4021 caccaagcac gccgccccgc gcattggcag accattgagc agccatttca gctaggtcaa
     4081 accttgcaaa cgcacacaaa agaagatgtt tgtattgtgg tcgattgcct cacactgtgg
     4141 ctcgcgcaat gcattttccc agattgcgac cgcccagcag acctcaactg gcaagatgag
     4201 cgccaagcca tgcttgacgt gctgccgaca ctcaaaggta acatcattct agtcagcaat
     4261 gaagtcggca tgggcatcgt gcctttgggc gaaatcaatc gccagtttca agacgaacaa
     4321 ggtcggctga accaagccgt ggcgcaaatc gcgcatcaag tcagttttat tgcggcaggt
     4381 ttgccgattg ttttaaaagg ataaccatgg aaaccacgac ttttgaactt agaggtgaat
     4441 tcatcgagct ttgccaatta ttaaaacttg tcggtattgc caatagcggt ggccagggca
     4501 aaatgatggt ggctgacggc attgtaaaag ttgatagtaa cgttgagttg cgcaaaactg
     4561 ccaaaattcg tgctgggcaa ttagtggaat gtttcggcca acaaattaag gtagaaaaac
     4621 cttaacttaa gcacaacttc tagcctgtta aagtggtata aaattatctc aaaacaccac
     4681 taaaaatcac acaacctttt tcgatgctct gtgagcctta taaataaagg cttccagagc
     4741 atattttttc tctcgggcgt tttgttgctt taaaaggtga tttttgaatc gtcataaaaa
     4801 agcgcgttat cggtcttcca gcacggctaa aaacgcatca ccataacgct gcaatttagc
     4861 ttgccccaca ccagtaattt ggctcatttc tgtcaagctg gcaggtttac gatttaatat
     4921 ttccagcaag gtgctgtcat gaaaaatcac ataaggcggc acgccctgct cttttgccaa
     4981 ctccatgcgt ttggctttga gtgcatgcca aagcgggtcg tcgctcacct ctgcataacc
     5041 ttctttggcg cgtgcgccgc gctcggcttt tgaagatttt cgactcacaa cttccacatc
     5101 gcgccttagc caaacttctg cttcgccttt caacaccggt ctggcagact ccgtcagttt
     5161 caggccacca taagcttcaa tgtcactcat caaatagccg cccgccacca gttgcctaaa
     5221 cacgctgctc cattgcgcct gagaaatggc cttgccaacc ccaaacacgc ttaagttttg
     5281 atggctaaat tggctgactt tgacattggt tttgcctagc agcacgtcaa ttaaatgcac
     5341 cacgccaaag cgctgcccgg tgcgatacac gcatgacagc gccatttgtg cggcttgcgt
     5401 ggcatcccat gtatccactg ggttaaggca gttatcacac tgcccacaat cgcccggatg
     5461 ctgctcgcca aaatagcgca agagcgtttg gtgacggcac tgcgtagatt cgcaaaagcc
     5521 taacagtgca tccagctttt gccgctctac ctgtttgcgt tcttctggcg cgtcgcctga
     5581 atcgagcatt ttgcgcatgc tcaccacgtc acccaaacca tacgccatcc atgcatttgc
     5641 gggcaagcca tcgcgacctg cacggccagt ttcttggtaa tatccttcca tactttttgg
     5701 caaatccaga tgcgccacaa aacgtacatt gggcttatca atgcccatgc caaacgccac
     5761 agtggccacc ataatcacgc cttcttcacg caaaaatcgg cgctgatttt tgttgcgcac
     5821 actcgcgtcc aaccctgcat ggtaaggcag tgcatcccat ccgcgcgact tcagccactc
     5881 agccgtttct tccacttttc tgcgtgataa acaatacaca atgccggcat cattggcatg
     5941 ttcatgttct aaaaaggttt ctaactgctg gcgcgcgctg gttttttgcg taacgtcttt
     6001 ttgcgtaatg cggtaacgaa tattcgggcg gtcgaagcta gaaacaaact gccgcgcttg
     6061 ttcaagcttc aaccgctcaa cgatttcggc gcgcgtgggc gcatcggcag tggctgtgag
     6121 tgcaatgcgt ggcacctgcg gaaagcgctc gtgcaacacc gtgagcttgc gatactccgg
     6181 ccgaaaatca tgcccccatt gcgaaacaca atgcgcttca tcaatggcga ataacgcaat
     6241 gctagcaccc tgttctattt gctccagcaa cgccaaaaag ctttccatca acaatcgctc
     6301 aggcgccaca tacacaatct gcaaatgccc acgcatcagt tgtgcggtca cttctcgtga
     6361 ttcattcgct tcttggctgg aattcaaaaa cgccgcccgt acgcccaatt gttttagtgc
     6421 atccacttgg tcttgcatca aggctatcaa tggagaaacc acaaccgcca caccttcacg
     6481 caacaaggct ggcaactgat aacacaaaga tttaccgcca cccgttggca tcaacaccaa
     6541 cgcatcgccg ccagcgctca cgtgggcgac aatcgcttct tgctcacccc gaaatgatga
     6601 atagccgaaa acgtcatgaa gaagttgctg tgcggtgttt gacatgaaat acccaaatag
     6661 cgtgcgtaag cccgccgtgc aatcaataaa acctagccaa agctgactag gttttgaatt
     6721 tggtagggcg tgcgaggatc gaactcgcga caaacggatt aagagtccgc tgctctacca
     6781 gctgagctaa caccccaatg acgagataat acactaatct agcgtgacaa ctaaaaccca
     6841 tgagctgatc gtctgataaa aaacgaaatg aaaaacgatt agtgctattt acttaattga
     6901 ctttaaatct gacagcagag tttttgtttt cttaaatttt cgacaagcct ctgagcaata
     6961 tttcacctct tgccacaccg ctttccattt cttccgccaa acaaaaggcc gatgacaagc
     7021 taaacataac ttagtgggaa aattggcttt tttgagcata tcaat
//
LOCUS       DAOQSI010000114         7060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_10435_length_7060_cov_6.586153, whole genome shotgun sequence.
ACCESSION   DAOQSI010000114 DAOQSI010000000
VERSION     DAOQSI010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 7060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7060
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10435_length_7060_cov_6.586153"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="PKL42_07650"
     CDS             <1..1111
                     /locus_tag="PKL42_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNU66987.1"
                     /translation="KDLPAVSPLPSASLVLVFGSRERFNDAKLPATLKARFPAAQIIG
                     CSTAGEISANGVLDDSLQITAIQWEKTVQRVTHTKMTSMQNSYETAADLARQLKSDEL
                     KAVLVYSDGLHVNGSELLEGFKSILGNTPIMGGMAGDGFNFSKTVQLFNDNVGEGLVI
                     AVGLYGRHLIAATGVGRGWKPYGPPRKVTKSNKNVVLELDGKPALPLYNMYIGAHSAK
                     GLPGSGLKFPFAIIEEGKRDIEKIRTLLAIDAKENSLTFAGNVDEGETVRFCQATHDR
                     LVEGAGDAAHLVTNHASTNQPGLAVCVSCVGRKGVMAENVADEVRLVQQILGPQTTLT
                     GFYSYGEFSPRPDTNDSVLHNQTMTIGYLSENLLA"
     gene            1272..1772
                     /gene="tpx"
                     /locus_tag="PKL42_07655"
     CDS             1272..1772
                     /gene="tpx"
                     /locus_tag="PKL42_07655"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNU66988.1"
                     /translation="MATVHLKGNPVQIGGNLPQNGQTAPDFQLADAKRNLVSLDAYAG
                     KRKVLNIFPSIDTPTCATSVRTFNQKASSVNNTVVLCISADLPFAQSRFCGAEGIENV
                     VTLSTFRDTAKFAKDYGVQILDSSLAGLTARAVVVLDENNQVLHSELVNEIADEPNYE
                     AALAVL"
     gene            complement(1814..2167)
                     /locus_tag="PKL42_07660"
     CDS             complement(1814..2167)
                     /locus_tag="PKL42_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HNU66989.1"
                     /translation="MSDSLVELRKRIDQFVTERDWAQFHTPKNLAMAMIVEAAELVEQ
                     FQWNTPEESQQLTPEKREAVSHELADTFVYLLRLAEVLEIDLIAAANAKIDLNAKKYP
                     VEKARGSNAKYTEYQ"
     gene            complement(2262..3422)
                     /locus_tag="PKL42_07665"
     CDS             complement(2262..3422)
                     /locus_tag="PKL42_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HNU66990.1"
                     /translation="MIPNRYFATCPRGLEQLLADELQTVKAKSIKLTDGGVSFDGDWA
                     VCYAANLHSRIATRILWQVARGKYVNEEDLFNAAYKLNWPQWFDVKHDFMVKVTGVKC
                     PLKSLEFATLKIKDAVCDKFRLAVGSRPYIDTKTPAVRIHAYLTAEEYQFYLDTSGSA
                     LYQRGNRGASIEAPLRENLAAGILKLSGWQPGQALLDPMCGSGTFLLEAAMVVLNIAP
                     GHKRSFGFEKLNTFEPNIWHQLKSQAAAKVKPVTFQKIYGSDVDLRAVRIAKRNLEEA
                     GLLAAVQLSQQDIVNVVPPADSGVLVANPPYGVRIGEGDDLALLYPKMGEALKRRFAG
                     WNTYFLTNDFTMPKLMRLTPSKRTPLFNGPLECRLFEIKMVAGSNRKDKNEG"
     gene            complement(3419..4033)
                     /locus_tag="PKL42_07670"
     CDS             complement(3419..4033)
                     /locus_tag="PKL42_07670"
                     /inference="COORDINATES: protein motif:HMM:NF018626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HNU66991.1"
                     /translation="MLKAGVVLLLLFNLQHAWASNLKLVEAARSQVGVTHSYDPQYTK
                     LAYPNGDVPLHKGVCTDVVIRALRDAHQLDLQQLVHEDMAKNFALYPKKWGLKKTDKN
                     IDHRRVPNLQVYFKRHWQSLGATQTAKDYQAGDIVTVMLPGNLPHIMLVSNKTEKNGP
                     SRAPTPLVIHNIGRGAQEEAALFAYPITGHYRLPKKYLTEESST"
     gene            complement(4026..4829)
                     /locus_tag="PKL42_07675"
     CDS             complement(4026..4829)
                     /locus_tag="PKL42_07675"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HNU66992.1"
                     /translation="MFRIAKHLMKRGNSDAVDVSETNGVRALHLGSPTIQSAMRIRDP
                     NALELTYTRGMMCFLLFNQNIKDVLAIGLGGGSLAKYMHAYCPEIISTVVEINPNIIQ
                     VARNQFYVPANDARLQVIEGDGLQYLADHANGADVLLIDAFDSNGIPPDFCSQDFFDM
                     CARSLKNAGIFAINLWGSDRNFDVYLQRIEHSFQQKVLILRTGRPGNIVVFGFNALYK
                     HFNIAQLQARAKLLESTHRIEFNQFLKQLMEDNIVQHNQLQFKRDDHDA"
     gene            complement(4829..6796)
                     /locus_tag="PKL42_07680"
     CDS             complement(4829..6796)
                     /locus_tag="PKL42_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HNU66993.1"
                     /translation="MNVFFEEDGSFKVASIMSETDSALQVESISGKRSKIKANHVLLR
                     FEAPLTGFLEAANQEAEPLDLDFLWECCGEEEFGFAEFAADYYGRKPSAVESAAIAIK
                     LHSAPVYFNRKGKGRYKAAPADILKAALAGLEKKRLLAEKMAHYVALLKAQQMPDELK
                     QKLDMLLYAPDKNAFEYKTLDAAANELHISHIKLLHACGAIPSVHDYHLGAFLREYFA
                     DGTDFKAMAATEALATLQFDDLPLAEAQAFSIDDSTTTEIDDAFSMKPLADGRMRVGI
                     HISAPALGIAVDSPLDVEAMQRLSTVYMPGHKITMLPEAVIRPFSLDAGTIKPVLSLY
                     LEVNADYTIAKRESKVERIHVAENLRHDTLEPYFNEETLSADSGHPYWANLLFLFNLS
                     EALEKARGKYDPSKPPQIDYNFYVTEGVVSIIGRRRGSPMDKLVAELMIEANQQWGAL
                     LAEHEVPGLYRAQTGGRVFMTTKAEPHLGLGVAQYAWSTSPLRRAVDLINQRQIISVV
                     QGTLPAYPPKSDALTTHMRNFELTYKSYSEFQARMERYWSLQYLIQEKIEEVHATVWR
                     DNIVRIDFPPLMTKVYGLPELKPGTRVGLKVQEVDTLLIEFRPKFIGVLEEAPAILAI
                     DEDEVFIEEPVAVVPPKPEESAPAEVNPEAT"
BASE COUNT         1821 a         1730 c         1588 g         1921 t
ORIGIN      
        1 taaagattta cccgctgttt cacctttgcc aagcgctagc ttagtgttgg tgtttggttc
       61 tcgagagcgt tttaacgatg cgaaattgcc tgccacttta aaggcgcgct ttcctgccgc
      121 acaaattatc ggctgctcta ctgctggtga aatttctgcc aatggcgtgc tggatgacag
      181 cttgcaaatc actgcaattc agtgggaaaa aaccgtacag cgtgtcacgc ataccaaaat
      241 gacgagtatg cagaactcgt atgaaacggc cgccgattta gcgcggcagt taaaatcaga
      301 tgaattaaaa gccgtgctgg tatattcaga tggcctgcat gtgaatggct cagaactgct
      361 tgaagggttt aagagcattc ttggcaacac gcctattatg ggcggcatgg cgggtgatgg
      421 ctttaacttt agcaaaaccg tgcagttatt taacgacaac gtgggtgaag gcttagtgat
      481 tgccgtaggt ttatacggcc ggcatttaat cgcggcaaca ggcgttggcc gcggctggaa
      541 accttatggc ccaccacgca aagtgactaa atctaacaaa aacgtggtgc tggaattaga
      601 cggcaaacca gcgctacccc tatacaatat gtacatcggc gcacattcgg caaaagggtt
      661 acctggctct ggcttaaaat ttccgtttgc gattattgaa gaaggcaagc gagacattga
      721 aaaaattcgc acacttctgg caattgatgc caaagaaaac agccttactt ttgccgggaa
      781 cgtggatgaa ggtgaaaccg tacgtttttg tcaggcaacc catgaccgct tagttgaggg
      841 cgcaggcgat gccgcacact tggtgaccaa ccacgccagt accaaccagc cagggttggc
      901 ggtttgtgtg agctgcgttg gccgtaaagg agtgatggcc gaaaatgtgg ccgatgaagt
      961 caggctggtt caacaaattt taggcccaca aaccacgtta accggttttt attcttacgg
     1021 tgaattctcc cctcgcccag atactaacga cagcgtattg cataatcaaa ccatgacaat
     1081 tggctattta agtgaaaact tacttgcctg atttgtttag ataaaaccct cattaaaaca
     1141 agggattaaa tccaaaataa atatgaaaaa gcccagtgca aactgggctt ttttgtttta
     1201 tgatgtgtgc atcatttcgt ttcatcgata ctgctttata acccctacgc aaaataacaa
     1261 aggagcacaa catggcaaca gttcatttaa aaggtaaccc ggtacaaatc ggcggtaatt
     1321 tgccgcaaaa tggtcaaaca gcacccgact ttcaactggc ggatgccaaa cgtaacttgg
     1381 tttcattaga cgcttatgct ggcaaacgca aagtgctcaa tatttttcca agtattgata
     1441 cgccaacttg cgccacttca gtacgcacat tcaaccaaaa agcgagcagc gttaataaca
     1501 ctgtggtgtt atgcatttca gcggatttgc catttgcgca aagccgtttt tgtggcgcag
     1561 aaggcattga aaatgtggtc acactttcca ccttccgcga taccgccaag tttgcaaaag
     1621 actacggcgt acagatttta gatagcagtt tagccggctt aaccgcacgc gcggtggtag
     1681 tgctggatga aaacaaccag gtattgcaca gtgaattggt gaatgaaatt gcagatgaac
     1741 ctaactatga agctgcgtta gcggtgcttt aagtcttaat ctaggagcgc aattgattgc
     1801 gctcctacac tagctattga tattcagtat atttcgcgtt actaccccgc gctttttcga
     1861 cggggtattt ttttgcattc aggtcaattt tcgcgttagc cgctgcaatc aaatcaatct
     1921 ctaacacttc ggccaatctc aacaaataaa caaacgtatc ggccaactca tggctcactg
     1981 cttcgcgctt ttcgggtgtg agttgctgac tttcttctgg tgtattccat tgaaactgct
     2041 cgaccagttc ggcggcttca acaatcatcg ccatcgccaa atttttaggt gtatgaaatt
     2101 gcgcccaatc gcgctcggtc acaaattggt caatgcgttt gcgcaattct acgagtgaat
     2161 ctgacatatc aaaacccctg attttgcatc gagaaaattt ttatgctctg cgagccttta
     2221 tttataaggc ttccagagca tcgaaaaagg ttgggctgaa attagccctc atttttgtct
     2281 tttcggttgc tacctgccac cattttaatt tcaaataacc ggcactctaa cggcccgtta
     2341 aataatggcg tacgtttgct tggcgttaag cgcatcagtt ttggcatggt gaaatcattg
     2401 gttaaaaagt acgtattcca gccagcaaaa cggcgtttta atgcttcacc catttttggg
     2461 tagagcaacg ctaaatcgtc accttcacca atccgcacgc cgtaaggcgg gtttgccacc
     2521 aacacgccgc tgtctgctgg cggcaccaca tttacaatat cttgttgtga aagctgcacc
     2581 gctgccagca agccggcttc ttccaaattg cgtttggcaa tgcgcaccgc acgtaaatct
     2641 acatctgagc cgtaaatttt ttgaaacgtt acgggcttca cttttgcagc agcctgtgat
     2701 tttaattgat gccaaatatt gggttcaaac gtgttgagtt tttcaaaacc aaaacttctt
     2761 ttatggccag gcgcgatgtt aagcaccacc atggcggctt ctaataaaaa cgtgccgctg
     2821 ccgcacatgg ggtctaacaa ggcttgtcct ggctgccagc ctgagagttt taaaattccg
     2881 gcggcaagat tttcacgcaa tggcgcttca atactcgcac cgcgattgcc gcgctggtag
     2941 agcgcgctgc ccgaagtgtc gaggtaaaac tggtactctt ctgccgttaa atacgcatga
     3001 atgcgcaccg ctggcgtttt ggtgtcaatg tacgggcgcg agccaacagc aagtctgaat
     3061 ttatcgcaca ccgcatcttt gatttttagg gtggcaaatt ccaagctttt taacggacat
     3121 ttaacgcctg tgaccttcac cataaaatca tgttttacat caaaccattg tggccaattg
     3181 agcttgtaag ccgcattgaa taagtcttct tcattcacat acttaccgcg cgcaacctgc
     3241 cacaaaatac gcgtggcaat tcgtgagtgc aaattagcgg catagcacac tgcccaatca
     3301 ccatcaaaac tcacgccgcc gtcagtgagt ttaatggatt ttgctttgac ggtttgtagc
     3361 tcatcagcca gcaattgttc aagcccgcgt gggcaagtgg caaagtatcg gttaggtatc
     3421 atgtgcttga ttcttctgtt aaatattttt ttggtaaacg gtaatgcccg gtgattgggt
     3481 aggcaaataa cgccgcttct tcttgcgcgc cgcggccaat gttatgaatc accagcggtg
     3541 tgggcgccct gcttggcccg tttttttctg ttttattgct caccagcata atgtgcggca
     3601 aattgccggg cagcatcacg gtgacaatat cacctgcctg ataatctttg gccgtttgcg
     3661 ttgcgccaag gctctgccag tggcgcttaa aatacacctg caaatttggc actcgccgat
     3721 ggtcaatatt tttatcggtc ttttttaggc cccatttttt agggtaaagc gcaaagtttt
     3781 ttgccatgtc ttcatgcacc agttgctgca aatcaagctg atgcgcatcg cgcaatgcac
     3841 gaatcaccac atcggtacac acgcccttat gcagcggcac atcaccattt gggtaagcca
     3901 gcttggtgta ttgcgggtcg taactgtgcg tgacacccac ttggctacgc gcagcttcta
     3961 ctaattttaa attgcttgcc caagcatgtt gcaggttgaa caacagcaat agaactacac
     4021 cagctttaag catcgtgatc atcccgttta aactgcaact gattgtgctg cacgatatta
     4081 tcttccatta attgttttaa aaattgatta aattcaatgc ggtgcgtgct ttcaagcaat
     4141 ttagcacggg cttgtagttg ggcaatatta aaatgtttgt acagtgcatt aaagccaaac
     4201 accacaatat tgccaggccg tccggtgcgc aaaatcagca ctttttgctg aaagctgtgc
     4261 tcgatacgct gtaaataaac atcgaaattt cggtcactgc cccataaatt gatggcaaat
     4321 atgccggcgt tttttaatga ccttgcacac atatcaaaaa aatcctggct gcaaaaatct
     4381 ggcggaatgc cattactgtc aaacgcatca atcagtagca catctgcacc atttgcgtga
     4441 tcagctaaat attgcaggcc gtcgccctca atcacttgca ggcgtgcgtc atttgctggc
     4501 acataaaact gatttcgcgc cacctgaata atattcgggt taatttcaac caccgtactg
     4561 ataatctcag ggcaatacgc atgcatgtac tttgccagcg agccgccacc aaggccaatc
     4621 gccagcacat ctttaatatt ctgattaaac agcaaaaagc acatcatgcc acgcgtatag
     4681 gtaagctcaa gcgcgttcgg gtcgcgaatg cgcatggcgc tttgaatggt gggcgaaccc
     4741 aaatgcagcg cgcgcacgcc attggtttca ctcacatcaa cagcatcact attgccacgc
     4801 ttcattaaat gttttgcaat acgaaacatt aggttgcctc agggttcact tcagcgggtg
     4861 cgctttcttc aggttttggt ggcacaacgg caacgggttc ttcaataaac acttcgtctt
     4921 catcaatcgc caaaatggca ggcgcctctt caagtacgcc aataaattta ggcctaaact
     4981 caatgagtag cgtatcgacc tcttgcactt ttagccccac gcgcgtgccc ggcttaagtt
     5041 ctggtaaacc gtagactttc gtcatcaatg gcggaaaatc tatgcgcacg atgttgtcac
     5101 gccaaacggt ggcgtgtact tcttcaattt tttcttggat taaatattgc aagctccagt
     5161 aacgctccat gcgcgcctga aattcactgt aacttttata agtaagctca aagttgcgca
     5221 tgtgggtggt gagcgcatca cttttgggcg gataggctgg cagtgtgcct tgcaccacgc
     5281 tgataatttg tcgctggttg attaaatcca ccgcacggcg cagtggcgac gtactccacg
     5341 catattgcgc tacgccaagc cctaaatgcg gttcagcttt ggtggtcata aatacacggc
     5401 cgcccgtttg cgcgcggtac aaacccggca cttcatgttc ggccagcaag gcgccccatt
     5461 gctgattggc ttctatcatg agttccgcca ccagcttatc catgggtgag ccacggcggc
     5521 ggccaataat gctgacaacg ccctcggtta cataaaagtt gtaatcaatt tgcggcggtt
     5581 tgctggggtc gtatttgccg cgcgcttttt caagtgcctc acttaaatta aataaaaaca
     5641 gcaagttagc ccaatacgga tgcccgctat cagcacttaa agtttcttca ttaaagtaag
     5701 gttctaaggt atcgtggcgc aggttttctg ccacgtgaat acgctcaact ttgctctcac
     5761 gcttggcaat ggtgtaatct gcattcactt ctaaataaag tgacagcacg ggcttaatgg
     5821 tacccgcatc taagctaaac ggccgaatca cagcttcagg cagcatggtg attttatggc
     5881 ccggcatata cacggttgat aaacgttgca tggcttccac atccagcggg ctatccaccg
     5941 caatgcccag cgccggcgct gaaatatgaa tgccaacgcg catgcgacca tcggccagcg
     6001 gcttcattga aaacgcatcg tcaatttcag tggtagtgct gtcatcaatg ctaaacgctt
     6061 gcgcctcggc cagcggcaaa tcatcaaatt gcaaagtggc aagcgcttcg gtcgctgcca
     6121 ttgctttaaa gtcagtccca tcggcaaaat actcacgtaa aaacgcgcct aaatggtaat
     6181 cgtgaacaga cggaattgcc ccgcaggcat gcaataactt gatgtggctg atatgtagct
     6241 cattggcagc ggcatctaat gttttgtatt caaaggcatt tttatctggc gcgtaaagca
     6301 gcatatcaag cttttgcttg agttcatcgg gcatttgctg cgcttttaaa agcgccacat
     6361 aatgggccat tttttcagcc agcaggcgtt ttttctcaag ccccgccagc gccgctttta
     6421 aaatgtcggc tggcgcggcc ttataacggc ctttgccttt gcgattaaaa tacacaggcg
     6481 cactgtgcag tttaatggcg atggcggcag attctactgc gcttggtttg cggccgtaat
     6541 agtctgcggc aaattcggca aaaccaaact cctcttcacc gcaacattcc cacaaaaaat
     6601 ctaaatcgag cggctctgcc tcttgattgg cggcttctaa aaagccagtg agcggcgctt
     6661 caaagcgcag caacacgtga ttcgctttaa ttttgctgcg cttaccgctg atggattcaa
     6721 cttgcagcgc gctgtcggtc tcgctcatga tgctggcaac tttaaaactg ccatcttctt
     6781 caaaaaatac gttcattatt tcaactcatt gccaattacc gccatgatac atgactcgct
     6841 aaacgattgc tgtgcttcac gcatcactta aacgctttta gtgagaatct tgcgcattaa
     6901 aattgccact ttttatacag ttaaaaatgc ctatttttta ggcaatttta acaccaacct
     6961 ttttcgatgc cctgtgagcc ttgtaaataa aggctcgcag ggcattattt tttctcgggc
     7021 gatgttaatg ccgattaaat cacatagacg caccttaatt
//
LOCUS       DAOQSI010000115         7029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_10508_length_7029_cov_4.455549, whole genome shotgun sequence.
ACCESSION   DAOQSI010000115 DAOQSI010000000
VERSION     DAOQSI010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 7029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7029
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10508_length_7029_cov_4.455549"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="PKL42_07685"
     CDS             complement(<1..430)
                     /locus_tag="PKL42_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNU66994.1"
                     /translation="MALLMGIDVGGTNLRIGIFDGLKLVDESRFQADFASICQKHSPK
                     VAWQQILNMTENAIRATQTKHADITAIGIGFPGFIDPVTKIIAQSPNLAGLKDVNLAA
                     NLSQRLGKKVVVENDANAAAYGEYCLALQPIGGLIYLGLGT"
     gene            complement(434..1924)
                     /gene="malQ"
                     /locus_tag="PKL42_07690"
     CDS             complement(434..1924)
                     /gene="malQ"
                     /locus_tag="PKL42_07690"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNU66995.1"
                     /translation="MISTHTPPLNQRRAGVLLHISSLPSDRNVGDLGTEAYRFVDFLR
                     EIGATVWQTLPINMPHADNSPYQALSAFAGNPDFISIETLQSQGLLLRKSDAEPQVFK
                     KVELLANAYEAFKIRGDKKEQLALTRFCKKQAHWLGDFALFLVLKKQFNQAGWNAWPD
                     EYKNRDAATLKHARKTLAHEIAVVQFTQYIFFTQWLKLKAYATKKKVFLFGDIPIFVA
                     YDSADVWAKPHLFKLDAHKNMAVVAGVPPDYFSATGQRWGNPHYNWDAMAADGFSWWV
                     SRLATQNALFDVVRIDHFRGLEAAWEIPANEDTAINGEWVLAPGDALLAAVKAALPHI
                     CLVAEDLGVITPEVDALRTQYDLPGMKILQFAFSGSDDNPYLPENIEGNSVVYTGTHD
                     NDTTLGWYYSLDDQQRTHFHEYLTQTQVQNQQVQMPEDLVHMALASNAMLAVIPMQDI
                     LALDGHHRMNTPGTCSGNWHWRFQWQQLSAEQQKNIKRAIKQSKRS"
     gene            complement(2071..3756)
                     /locus_tag="PKL42_07695"
     CDS             complement(2071..3756)
                     /locus_tag="PKL42_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HNU66996.1"
                     /translation="MHQPDYRDTVTNEYVLPWTYLHAIKDYSDMAYHLEENPKARVTF
                     NFVPILLEQLEDYTNQFKENQIRDPLLSLLILEDLAGISPEQCRLIVTSCFKSHHEKM
                     LSPFPHYQRLLHLYQLIEPIVGVDDFHYLSAQYKVDLLVWYHLAWCGESLRRNSKVIQ
                     RLMAKGVLFTYEDRRQLFTTIGDTISGLIPRYKALLASQQIEISTTPYYHPILPLLLD
                     FKSTLDVMPYAPLPKHAKYAGGQPRATAHVVKADSYHSQLFGEKPRGMWPAEGAVSNA
                     ALSLLAEHGVEWAATGQGVLANSLLKSQLSVNHKVDYLYQPYRVTNGKQDILCFFRDD
                     ALSDKIGFEYAKMHTPDAVSDFVKTLENIAKSDGPAKKVVSVILDGENAWEYYPYNGY
                     YFLNELYAALSNHPDIVMTTFSDIVDLYQSAEYKSKGIVAPVLPQVAAGSWVYGTFST
                     WIGSKDKNQAWDLLCEAKKTYDQVIAKNGISAEKLAACERQLAICEGSDWFWWFGDYN
                     SSDSVASFDQLYRRNLMNLYKLLGQPVPQVLHVKISAGGGNADNAGTMRRGQE"
     gene            complement(3909..5201)
                     /gene="glgC"
                     /locus_tag="PKL42_07700"
     CDS             complement(3909..5201)
                     /gene="glgC"
                     /locus_tag="PKL42_07700"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNU66997.1"
                     /translation="MAPNRPIEQHSDRFISNLTKQTAAIILAGGRGSRLKSLTDWRAK
                     PAVQFGGKFRIIDFPLSNCINSGIRRINVATQYKAQSLIQHIQRGWGFLRGEFNEYVN
                     IIPAQQRISEDWYKGTADAVFQNIDLLREGGAEYVLILAGDHIYKMDYGKMLATHALN
                     HADMTVACINVPLEDAKGFGVLAVDNTDRVIEFAEKPANPKHMPDDPTKAFASMGIYI
                     FNAKFLYEQLIRDAGDPKSSHDFGGDIIPYIIKKYKVQAHRFTDSCVGAKNGNHYWRD
                     VGTIDAYWEANMELTKVIPELNLYDKEWPIWTYQEQLPPAKFVFNDEGRTGKATDSLV
                     SGGCIISGSSVTSSVLFSDVRVHSYCDIEASVILPKVKVNRNVVLKNAVIDRGCIIPE
                     GMQIGVDLALDAKRFYVSEKGITLVTPDMLGQDLYRVI"
     gene            5476..>7029
                     /gene="glgB"
                     /locus_tag="PKL42_07705"
     CDS             5476..>7029
                     /gene="glgB"
                     /locus_tag="PKL42_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HNU66998.1"
                     /translation="MLKAKHHDPFSYLGCHEVDGSYAYRTFLPPATEVSIKLDSKWIV
                     LEKTHADGLFETVSKKPFKTPCLLKVTTGESAFEIYDPYTFNSSLTQDELYLLGEGRL
                     LQAYKTLGAQFRTQEGIAGVRFAVWAPNAERVSVTGNFNNWDGRIHSMRAHGSSGVWD
                     IFIPHLTTFDTYKFEIRNRHTGAVLVKTDPYGFEFEQRPGTAAKISLSQHQWNDDAWL
                     DARKNRDWLHAPFNCYEVHLGSWQRNESGHFLSYRDLATTLVPYVAEMGYTHVEFMPI
                     SEHPLTESWGYQTTGYFGVTNRFGSPDDLRFLIDAFHQANIGVILDWVPGHFPKDDWA
                     LARFDGTALYEHEDPRLGEHQDWGTYIFNYGRNEVRNFLMANAHFWLSEFHIDGLRVD
                     AVASMLYLDYSRKAGEWLPNKYGGRENLDVIEFLKQLNVMVGEDFAGVLTIAEESTAW
                     PAVSRPVYLGGLGFSMKWNMGWMNDTLSYIEHDPVHRRYYHDKLTFGQLYAYSENFVL
                     PFSHDEVVHG"
BASE COUNT         1970 a         1633 c         1444 g         1982 t
ORIGIN      
        1 ccgtacctaa gcctaaataa atcagtccgc caatcggttg taaagccaag caatattcac
       61 catacgctgc ggcattcgca tcattttcca ccacaacttt tttgcctaag cgttgcgata
      121 aattggccgc aagattcacg tcttttaaac cggctaaatt tggcgattgc gcaataattt
      181 ttgtcactgg gtcaataaac cccggaaaac caatgccaat cgcggtaata tccgcatgct
      241 tggtttgtgt agcgcgtata gcattttcag tcatattaag aatttgctgc caagctactt
      301 taggggagtg tttttggcaa atgcttgcaa aatcggcttg aaatcggctt tcatctacca
      361 gcttcaaacc atcaaaaata ccaatgcgta aattcgtacc acccacatca atacccatta
      421 acaatgccat cacctagctc cgcttactct gcttaatggc gcgtttgatg tttttttgtt
      481 gttccgctga caattgctgc cattgaaaac gccaatgcca attgccggag caagtgcctg
      541 gggtattcat gcgatgatgc ccatcaagcg ccaaaatatc ttgcatggga atcactgcca
      601 acatggcgtt ggaggccagt gccatatgca ctaaatcttc aggcatttgc acttgctgat
      661 tctgcacttg tgtttgcgtg aggtattcgt gaaaatgtgt acgctgttgg tcatctaagc
      721 tgtaatacca acctaaagtg gtgtcgttgt cgtgcgtacc ggtataaaca acgctattgc
      781 cttcaatatt ttcaggtaga tatgggttgt catctgagcc actgaaagca aactgtaaaa
      841 ttttcatacc aggtagatcg tattgagttc ttaatgcgtc tacttctggc gtgatgacac
      901 ctaaatcttc agctactaag cagatatgcg gcagtgctgc cttgactgcg gccagtaacg
      961 catcaccggg tgccagtacc cactcgccat tgatcgcagt atcttcgttg gctggaatct
     1021 cccacgccgc ctccaaccct ctaaaatgat caatacgcac cacatcaaac aatgcatttt
     1081 gcgtcgccaa tcgtgacacc caccagctaa agccatcggc ggccatcgca tcccagttgt
     1141 aatgcgggtt accccagcgc tggcctgtgg cagaaaaata atctggcggc acaccggcca
     1201 ccaccgccat atttttatgc gcatcaagct taaataaatg cggtttcgcc catacgtcag
     1261 cactgtcgta ggccacaaat ataggaatat caccaaacaa aaatactttc tttttcgtag
     1321 catacgcctt tagctttaac cactgtgtga agaaaatata ctgtgtgaat tgtacgactg
     1381 caatttcgtg cgctaaggtt ttacgcgcat gtttaagggt cgcagcatcg cgatttttgt
     1441 attcatctgg ccacgcattc cagcccgcct gattaaactg ctttttgagt accaaaaaca
     1501 acgcaaaatc acctagccaa tgtgcctgtt ttttacaaaa tcgcgttaaa gctaactgct
     1561 cttttttatc tccccgaatc ttaaaagctt cataggcgtt ggctaacaat tctacttttt
     1621 taaagacttg gggttcagca tctgattttc ttaataaaag cccttgcgat tgcagcgttt
     1681 cgatactgat aaaatcagga tttcccgcaa aggctgataa tgcttgataa ggtgagttat
     1741 cagcatgcgg catattaatg ggcaatgttt gccaaacggt tgcgccaatt tctcgtaaaa
     1801 aatcgacaaa acgataagct tcagtgccta aatcgcccac attacggtcg cttggcaaag
     1861 agctaatgtg caatagaaca ccagctcgac gctgattcaa tggaggcgta tgggtagaaa
     1921 tcatgaaagt ctcaaataat tttttatcgc tgcgtgacgc agaaagattt aatgttgtaa
     1981 aaaataatgc tcatatctca aataagacga ttcgactaaa taacttaaaa agaagcgtta
     2041 aaaaacgcca atattcaggc gagctttaaa ttactcctgc ccacgacgca tagtgcccgc
     2101 gttatctgca ttgccgccac ctgcgctgat tttgacatgt aacacttgcg gcacaggctg
     2161 acccagcagt ttatataaat tcatgagatt gcgacgatag agctgatcaa aactcgcgac
     2221 gctatcagat gagttgtagt caccaaacca ccaaaaccaa tcagaccctt cacaaatggc
     2281 tagttgacgc tcacatgcgg cgagtttttc agccgaaata ccatttttgg caataacctg
     2341 gtcgtacgtt ttcttagcct cacacagtaa atcccaagcc tgatttttat ctttactgcc
     2401 aatccaagtg ctaaatgtgc catacaccca gctgccggca gctacttgtg gcagtaccgg
     2461 tgccacaata cctttactct tatattctgc cgattgatat aaatctacga tgtcactaaa
     2521 ggttgtcatc acaatatcag gatgatttga aagtgccgca tacaactcat ttaaaaagta
     2581 atagccgtta tagggataat attcccaggc attctcacca tctaaaatga cactcaccac
     2641 ttttttagct ggcccgtctg atttggcgat attttctaat gtttttacaa aatcgctcac
     2701 cgcatcgggc gtgtgcattt ttgcatattc gaaaccaatt ttgtccgaaa gggcatcgtc
     2761 tctaaaaaaa cataaaatat cttgtttacc gttagtaacg cggtatggct gataaagata
     2821 atccacctta tgattgacac taagctgaga ttttaacaaa ctgtttgcca gcacaccctg
     2881 ccctgttgcc gcccactcaa ccccatgttc tgccagcaat gaaagcgccg catttgacac
     2941 agcaccttct gctggccaca ttccgcgcgg cttttcacca aataactgtg agtggtatga
     3001 atcggctttt accacatgtg ctgttgcgcg tggctgccca ccggcatatt ttgcgtgctt
     3061 gggcaaaggc gcataaggca tcacgtccaa agtcgattta aagtccaaca ataaaggcag
     3121 aattggatgg tagtaagggg ttgttgaaat ctcaatctgc tgactagcca gtaatgcttt
     3181 atagcgcggt atcagaccgg aaattgtatc cccgatagtg gtaaacaatt ggcgtctatc
     3241 ttcgtaagta aaaagcaccc cttttgccat caacctttgg atgactttgc tattgcgtct
     3301 taagctttcg ccacaccaag ctaggtgata ccagaccaac aaatctacct tatattgcgc
     3361 tgataaatag tgaaaatcat ccacccctac aatcggctca attaactggt agagatgcaa
     3421 taatcgttga tagtgtggaa atgggcttag cattttttcg tgatggcttt taaagcagct
     3481 cgttacaatc agccgacact gctctgggct aatgcccgct aaatcctcaa gtatcaataa
     3541 gcttagcaaa ggatcgcgaa tttgattttc tttaaactgg ttggtgtaat cttctagttg
     3601 ctcgagcaga atcggaacaa aattaaacgt gacgcgggct tttggatttt cttctaagtg
     3661 ataagccata tcactgtaat cttttatggc atgcaggtag gtccaaggca gcacatattc
     3721 attcgtcacc gtatcacggt aatctggttg atgcatatgc caatatagat tgaggagaac
     3781 ttttgaacga ttagatttca ctttgaatga cgacctttaa aacaaaaaac gaataacttc
     3841 aaattgacat cggtgcgtaa atttttacgc accgatgaat taggcaccgg ctaattaggt
     3901 atggtaaatt atatgacgcg atacaagtcc tgacctagca tatcaggggt gaccaaggta
     3961 atcccttttt ctgaaacata aaaccgtttg gcatctaacg ccaaatcgac gccaatctgc
     4021 atgccttcag ggataataca tccgcgatca attaccgcat tttttaacac aacatttcga
     4081 tttactttta cttttggcaa tatcaccgaa gcttcaatat cgcaataact atgcacgcgc
     4141 acatcggaaa acagtactga actggttacg ctcgaaccac taataataca gccgcctgaa
     4201 accaatgaat cggttgcttt gcctgttcgg ccttcatcat taaaaacaaa ctttgccggg
     4261 ggtaattgct cctgatatgt ccagataggc cactctttat catagagatt taattcagga
     4321 atcaccttgg tgagttccat attagcttcc caataggcat caatcgtacc gacatcacgc
     4381 cagtaatggt taccgttttt agccccaaca caactatcgg taaaacgatg cgcctgaact
     4441 ttatactttt taatgatgta cggaataatg tccccaccaa aatcgtggct agattttggg
     4501 tcgcccgcat cgcgaatcaa ttgttcatag agaaattttg cattgaaaat atagatcccc
     4561 atgcttgcaa atgcctttgt cgggtcatct ggcatatgtt ttggattagc cggtttttca
     4621 gcaaactcaa tcacgcgatc tgtattatcc accgccagta caccaaagcc tttggcatct
     4681 tctaatggca cgttgatgca agccacggtc atgtcggcat gattaagcgc atgcgtcgcc
     4741 agcattttac cgtagtccat tttatagatg tggtcacctg ctaaaatcag cacatactcc
     4801 gccccaccct cacgtaacaa gtctatgttt tgaaacaccg catcagccgt gcctttgtac
     4861 caatcttccg agatacgctg ctgcgctgga ataatattga cgtattcatt aaattcacca
     4921 cgcaaaaatc cccaaccgcg ctgaatatgc tgaatcaggc tttgcgcctt atattgtgta
     4981 gccacgttga ttcggcgtat gccagaatta atgcaattag ataatggaaa atcaataata
     5041 cgaaacttac caccaaactg caccgctggt ttcgctcgcc aatctgtaag gctttttagt
     5101 cggctaccgc gcccgccggc taaaataatc gccgcggtct gcttggttaa gttactgata
     5161 aaacgatcag aatgttgttc tattggtcga tttggcgcca tgatgccctc ctgaagttta
     5221 atttatattg acaatccgtt taactagccg ggctaactag ctttaatttt ctatttaact
     5281 ggtcttcata tacattataa tcagcttaaa gcactataat ctagcaataa cagcagaaaa
     5341 gttaaaaata tttcttaaaa aatttaaggg gaatgtctct tggtcaagtc cactacaacg
     5401 cctagccaaa aacgtgagaa aagcacgaaa actactagag aaaaacccgc tcacttagct
     5461 gatggtctta agcgcatttt aaaagctaag caccatgacc cattcagcta tttgggttgt
     5521 catgaagtgg atggctcata tgcgtatcga acgtttttgc cgccggcgac tgaagtgtca
     5581 attaagcttg atagtaaatg gattgtgtta gaaaagaccc atgcagatgg cttatttgaa
     5641 actgtaagca aaaaaccttt taaaacgcct tgcctgctga aagtgacgac gggtgaaagc
     5701 gcatttgaaa tatatgaccc ctacaccttt aattcaagct taacgcagga tgaattgtac
     5761 ttactaggtg aaggtcgctt gttgcaagca tataagacac ttggcgcaca attcagaaca
     5821 caagaaggca tcgcgggcgt tcgttttgcc gtgtgggctc caaacgctga acgtgtcagc
     5881 gttactggca actttaacaa ttgggatggc cgcattcact ccatgcgtgc gcatggctcc
     5941 agcggcgtat gggatatttt tattccacac ctcaccacgt ttgataccta taagtttgaa
     6001 attcgtaatc gtcatacagg tgccgtactg gtaaaaaccg atccctatgg ctttgagttt
     6061 gaacaacgcc caggcactgc agcaaaaatt agccttagcc aacaccaatg gaatgatgat
     6121 gcttggttag atgcgcgcaa aaatcgggat tggttacatg caccgtttaa ctgttatgaa
     6181 gtacatttgg gttcatggca gcgcaatgaa agcggtcact ttttaagtta tcgtgatttg
     6241 gcaacaacgc tagtgcccta tgtcgcggaa atgggttaca cccatgttga attcatgcca
     6301 atttcagagc atccgctcac ggagtcatgg ggttatcaaa caaccggcta ttttggcgtc
     6361 accaatcgct ttggttcgcc agatgatttg cgctttttaa ttgatgcttt tcatcaggcc
     6421 aatatcggcg tgatattaga ctgggtgcca ggccatttcc ccaaagatga ttgggcattg
     6481 gcgcgttttg atggcacggc tttatacgag catgaagacc cacgtttagg tgaacaccaa
     6541 gactggggta cgtatatttt caattacggc cgcaatgaag tgcgcaattt tttaatggcg
     6601 aacgcacatt tttggttatc tgaatttcac atcgatggct tacgtgttga tgcggtagct
     6661 tcaatgctct acttagatta ctcacgcaaa gcaggtgaat ggctgccaaa caagtacggc
     6721 ggacgtgaaa atctagatgt cattgaattt ttaaaacaat tgaacgtcat ggtcggtgaa
     6781 gactttgccg gtgtactcac cattgcagaa gaatccactg cgtggcctgc cgtatcgcgt
     6841 ccagtctatt taggcgggtt gggctttagc atgaagtgga atatgggatg gatgaacgat
     6901 acgctctcct atattgaaca cgacccggtg cacagacgct attatcacga caaactcacc
     6961 ttcggtcagt tatacgccta ttctgaaaac tttgttctgc ctttttcgca tgatgaagtt
     7021 gtgcatggc
//
LOCUS       DAOQSI010000116         6935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_10729_length_6935_cov_5.242442, whole genome shotgun sequence.
ACCESSION   DAOQSI010000116 DAOQSI010000000
VERSION     DAOQSI010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6935
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10729_length_6935_cov_5.242442"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="PKL42_07710"
     CDS             complement(<1..360)
                     /locus_tag="PKL42_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HNU66999.1"
                     /translation="MSNDLQGHRSSYQDLASWLQYIESLHPKSIAMGLERITRMIERL
                     QLNPAFKIITVAGTNGKGSTCAMLEQIYLQAGFSVGCYTSPHLLRYNERVRVNGHELT
                     DEALCKAFSVIDAARNSH"
     gene            complement(402..1265)
                     /gene="accD"
                     /locus_tag="PKL42_07715"
     CDS             complement(402..1265)
                     /gene="accD"
                     /locus_tag="PKL42_07715"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HNU67000.1"
                     /translation="MGWLNQLPQKIKRAVGGDKKSVPEGVWSKCSSCQSALYHKDLAD
                     NAEVCPKCGYHNRIGARARLAQLLDSEGQFEIGANVQAVDVLKFNDSKSYADRIKESQ
                     KAVNEKDALVVMQGAIKSVPAVVAVFEFKFMGGSMGSVVGERFVRGVQTAIDNKMSFI
                     CISASGGARMQEGLFSLMQMAKTSAALTQLSKAGLPYISVLTDPTMGGVSASFAMLGD
                     VIVAEPQALIGFAGPRVIEQTVRETLPDGFQRAEFLLEHGAIDMIIDRREMRDRLASI
                     LANLMRLPIAA"
     gene            complement(1346..2155)
                     /gene="trpA"
                     /locus_tag="PKL42_07720"
     CDS             complement(1346..2155)
                     /gene="trpA"
                     /locus_tag="PKL42_07720"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNU67001.1"
                     /translation="MSRIQATFKTLKQQGKTALIPYITAGDPHPKHTVNLMHTLVKNG
                     ADMIELGIPFSDPMADGPVIQRASERALVHKVGLTAVLEMVKAFRQTDQATPIILMGY
                     ANPIEAIGAVNFADRAKAADVDGVITVDYPPEECGEFVKELRARDIDPVFLLSPTTEP
                     SRIDLIVNQASGFVYYVSLKGVTGAANLDIAEVSQRVASIRKQTNLPVGVGFGIRDAV
                     TAKATAAIADAVVVGSRMVQVIEASNNENLLNNVATLMLELKAAVDAAVKI"
     gene            complement(2175..3377)
                     /gene="trpB"
                     /locus_tag="PKL42_07725"
     CDS             complement(2175..3377)
                     /gene="trpB"
                     /locus_tag="PKL42_07725"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNU67002.1"
                     /translation="MKLYDMPDARGHFGQFGGTFVAETLVEALDELRLMYDKYRHDPE
                     FLAEFAYDLKHFVGRPSPIYHAKRLSDKVGGAQIYLKREDLNHTGAHKINNTIGQALL
                     AKRMGKPRVIAETGAGQHGVATATIAARLGLECVVYMGSEDVKRQAPNVFRMKLLGAT
                     VVPVESGSKTLKDALNEAMRDWVTNVHNTFYIIGTVAGPHPYPMMVRDFQAVIGTEAK
                     VQMMEMVGRQPDAVVACVGGGSNAMGIFYPYIDVPNVRLIGVEAAGHGLETGMHAAPL
                     TANSPVGVLHGNRTYLMQDADGNIIETHSVSAGLDYPGVGPEHAWLKDIKRAEYVAIT
                     DDEAMAAFHNLCRTEGIIPALESSHALAYAEKLAKTMRKDEIVLVNLSGRGDKDINTV
                     AKLANITL"
     gene            complement(3393..4040)
                     /locus_tag="PKL42_07730"
     CDS             complement(3393..4040)
                     /locus_tag="PKL42_07730"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNU67003.1"
                     /translation="MFRTRVKICGITRLEDALVAVDLGVDALGLVFYAPSPRNVTIAT
                     AADVARQIPAFVSVVGLFVNAEPSFVEEVIANVGLSLLQFHGDETPEDCERFGLPYIK
                     AIRVKADTNLVQYARDFTSAKALLLDTYTEGVAGGTGQMFDWNLIPSALPKPVILAGG
                     LQANNVAQAIWQVKPYAVDVSGGVEATKGIKDAQKIAAFMQQVNLSGNKEYLDRI"
     gene            complement(4096..4917)
                     /gene="truA"
                     /locus_tag="PKL42_07735"
     CDS             complement(4096..4917)
                     /gene="truA"
                     /locus_tag="PKL42_07735"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNU67004.1"
                     /translation="MTRIALGVAYDGSGFCGWQSQPSACGVQDAIEAAIASISQHAVR
                     VHAAGRTDTGVHASCQVIHFDTESTRPISAWVRGVNANLPETVRIEWAVEVDSAFHAR
                     FSAYSRRYEYLLYNAPVASALMAKKSGWFHLPLDYSAMQKAAGYLIGEHDFSAFRASE
                     CQASSPVRTMHQAEVTQTKLTENKAYFQFNFSANAFLQHQVRNMIGALIYIGKGAHPP
                     EYMQTLLQSRNRTLSPPTFSPHGLYLIGVGYDAKWGLPIGEREPVAVNKNLSIRI"
     gene            complement(4914..5567)
                     /locus_tag="PKL42_07740"
     CDS             complement(4914..5567)
                     /locus_tag="PKL42_07740"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HNU67005.1"
                     /translation="MHPFVKILSFIMILIGMSHMSQVYLGALCVISCTIAFVFARKSF
                     SQVVLRMRWLFLSILIVYAFATPGEYIQPFLFDTAPTYEGMQSGLLQVGKLLIAIASL
                     SILFATCSQMQLMTGLYYLLLPLQWLGLNVERFTARLLLTLDYVETLALDKNFKFNVQ
                     QFDRLHAVEKPLMPEINALKQIELAFLPFKLRDKFALVAIALCFFWLVLLSVKAASI"
     gene            complement(5874..>6935)
                     /locus_tag="PKL42_07745"
     CDS             complement(5874..>6935)
                     /locus_tag="PKL42_07745"
                     /inference="COORDINATES: protein motif:HMM:TIGR03504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HNU67006.1"
                     /translation="PDNPLYDVSKLAAVSEGASETLDQVNDENDLVFGSDEVTQKAED
                     NNLDFSLDSDEVLNDPFADQASASVEDAASVVADSFAPADNEIAFTSDTETFDLGDLG
                     GTDTSQTANAIENSLQSEVSNGMDFDLGDAFDIGEPVASASVATENIADSLPSFDLPV
                     AASTDALDAPALDLSSEFTSESLENAEQVTTAEAPALDFAMDFDFPASTETSAPASAP
                     AVNLAEEKAAVESIGDFSFDLPDTEADNVITANESASIVSAPEAHTFDLSSINLDLDT
                     PTLQPTASQVDDFSTEGLAPANNVGLEPQDVEIKLDLVAVYIDMDDKVGARELLEEVL
                     KEGSVQQKQRAQTLLDSLA"
BASE COUNT         1916 a         1740 c         1415 g         1864 t
ORIGIN      
        1 atggctattt ctggcagcat caatcactga aaaagccttg cataaagctt catctgtcaa
       61 ctcatgcccg tttacgcgta cacgttcgtt gtagcgtaat aaatgtggcg acgtatagca
      121 gcctacagaa aaacccgctt gcagataaat ttgctctaac atcgcacaag ttgagccttt
      181 gccgttcgtg ccagcaacgg taataatttt aaaggccggg ttgagttgca ggcgctcaat
      241 catacgggtg atacgctcta gccccatcgc aatcgatttg gggtgcaagc tttcgatgta
      301 ttgaagccaa gacgctaaat cttgataaga tgaacggtga ccttgcaggt catttgacat
      361 cttttgctcc atgaaggaag tgtttactta atcgcccagc actaagcagc tattggcaag
      421 cgcatcaggt tagccaaaat tgacgccagt cgatcacgca tttcacgacg gtcaataatc
      481 atatcaatcg ccccatgctc taataaaaac tcagcgcgct gaaagccatc tggtaaggtt
      541 tcacgtacag tttgctcaat cacgcgtggc ccagcaaaac caatcagcgc ctgcggctcc
      601 gccacaatca catcgcccag catggcaaaa ctcgctgaaa caccgcccat ggtaggatcc
      661 gtcagcacgg aaatatacgg caaaccggcc ttgcttaatt gtgtgagtgc ggcactggtt
      721 ttcgccatct gcatgagtga aaacagacct tcttgcatac gcgccccacc actggcagaa
      781 atgcaaataa aggacatttt gttatcaatg gcggtttgca caccacgcac aaagcgctca
      841 cctacgacag agcccatcga gccgcccata aatttaaact caaacactgc aaccaccgcc
      901 ggcactgatt tgatggcgcc ttgcatgacc accaaagcat ctttttcatt caccgctttt
      961 tgtgattctt taatacggtc agcataactt ttgctgtcgt taaatttaag tacgtctaca
     1021 gcctgtacat tcgcgccaat ttcaaactga ccttcggaat ccagcaactg cgccaaacgt
     1081 gcacgtgcac cgattcgatt gtgataaccg cacttagggc acacttctgc attatctgcc
     1141 aagtccttgt gatacaaggc agattgacat gaagaacatt tgctccaaac gccttcagga
     1201 acactttttt tatctccgcc tacagcgcgt ttaatttttt gtggtaattg attgagccaa
     1261 cccataagta cacccctttt tttagcttaa tttgctgatc ttgatttgcc agtctaaatt
     1321 tcaagtctaa gcgctaatgt tcactttata tttttacagc agcatcgact gcggctttta
     1381 gctcaagcat taatgttgca acgttattga gcaagttttc attatttgac gcttcaatca
     1441 cctgcaccat tcgactgcct accaccactg catcagcgat ggctgccgtg gcttttgcag
     1501 tgactgcatc gcgaatacca aagcccacgc ctacaggcaa gtttgtttgc ttgcgaatgc
     1561 tcgccacgcg ttgtgagact tctgctatat ccaaattagc cgcgcctgtc acccctttga
     1621 gcgacacata gtaaacaaag ccactggctt gattcactat taaatcaatg cgtgaaggct
     1681 cagttgtggg tgaaagtaaa aacaccggat caatatcacg cgcgcgtaac tcttttacaa
     1741 attcgccaca ctcttctggt ggatagtcaa cagtaatcac accgtcaaca tctgccgctt
     1801 ttgcacggtc tgcaaaattg acagcaccaa tcgcctcaat cgggttagca tagcccatta
     1861 aaataatcgg cgtagcttgg tcagtttggc gaaacgcttt taccatttca agcacggcgg
     1921 ttaacccaac tttatgcact agtgcacgtt cacttgcgcg ctgtatcacc gggccatcgg
     1981 ccatggggtc ggaaaatggg atccccaatt caatcatatc tgcaccattt ttgactaagg
     2041 tatgcatcaa attgacggtg tgtttagggt gcggatctcc tgctgtaatg taggggataa
     2101 gtgccgtttt accctgttgc tttaaggttt taaaagtagc ttggattctt gacatgggat
     2161 tattggattt aaacttataa agtaatgttt gctaatttag cgactgtatt gatgtcttta
     2221 tcgccacggc cagataaatt cactaacacg atttcatctt tgcgcatggt tttagcgagc
     2281 ttttctgcat aggctaacgc atggctggat tctaatgccg gaataatacc ttccgtacgg
     2341 cataagttgt gaaaagccgc catcgcttcg tcatctgtaa tggcaacata ttcagcacgt
     2401 ttaatgtctt taagccaagc gtgttcaggg cccacaccgg gataatctaa accagccgaa
     2461 actgagtgtg tttcaataat attgccatca gcgtcttgca ttaaataggt acgattgccg
     2521 tgcaatacac cgactggact attcgcagtc aacggtgcag catgcatgcc tgtttccaac
     2581 ccatgcccgg ctgcctctac gccaattaag cgcacatttg gcacatcaat ataaggataa
     2641 aaaatgccca ttgcatttga gccgccaccc acacaggcaa ccacggcgtc tggttggcga
     2701 ccaaccattt ccatcatttg cacttttgcc tcagtgccta tcacagcctg aaaatcacgc
     2761 accatcatcg ggtagggatg cggaccggcc acagtgccga taatataaaa agtgttatgc
     2821 acgttggtga cccaatcgcg catcgcttca tttaaggcat ctttcagcgt tttagagcca
     2881 ctttctactg gcaccaccgt agcccctaac aacttcatcc tgaacacatt cggtgcttgg
     2941 cgcttgacat cctcgctccc catgtaaact acgcattcaa gacccaaacg agcagcaatg
     3001 gtggcagttg caacaccatg ctggcccgca ccagtttctg caataacgcg cggtttgccc
     3061 atgcgtttag ccagcaaagc ctgaccaatg gtattgttaa ttttgtgcgc gccagtgtgg
     3121 tttaagtctt cacgctttaa ataaatttgt gcgccaccaa ctttatcaga caaccttttg
     3181 gcatgataaa ttgggcttgg acgtcccacg aagtgtttta agtcgtaggc aaattctgct
     3241 aaaaactcag ggtcatggcg atatttatca tacatcaaac gcagttcgtc taatgcttca
     3301 accaaggttt ccgcaacaaa agtaccaccg aattgtccaa aatgcccacg tgcatcgggc
     3361 atatcataaa gcttcatgca tcactcccaa ctctaaattc tgtctaaata ttctttgttg
     3421 ccacttaaat ttacttgctg cataaatgct gcaatctttt gagcgtcttt tatgcctttt
     3481 gtagcctcaa cgccgccgct tacatccacc gcataaggtt ttacctgcca gatggcctgt
     3541 gcgacattat ttgcttgtag gccgcctgcc aatatcacag gctttggcaa cgcgctggga
     3601 attaaattcc aatcaaacat ttgccctgtt ccaccagcca cgccttcggt gtaggtgtct
     3661 agcaataaag cttttgctga tgtaaagtca cgggcgtatt gtaccaaatt tgtatcggct
     3721 ttgacgcgaa ttgctttgat atatggcagg ccaaaacgtt cgcagtcttc tggcgtttca
     3781 tccccatgaa attgtaaaag actaagcccc acgttagcaa tcacctcttc aacaaagctt
     3841 ggctcagcat ttacaaacaa gccaaccaca ctcacaaacg caggaatttg gcgggcgaca
     3901 tctgcggcag tggcaatggt tacgtttctg ggacttggcg cataaaacac caatccaagc
     3961 gcatccacac ctaaatctac tgctactaag gcatcttcta gccgcgtgat accgcaaatt
     4021 ttaactctag ttctgaacaa atttagcccc aaaaaatcat tttaaaacgc gttaaatcaa
     4081 gtctcattgt aacgcttaaa tccgtatgct cagattttta ttcacagcta ccggctcgcg
     4141 ctcaccaata ggcaaccccc atttagcgtc gtaacccacg ccaattaaat ataagccgtg
     4201 cggtgaaaaa gttgggggtg acaaggtgcg attacgactt tgcaacaagg tctgcatata
     4261 ttctggcgga tgcgcaccct taccaatata aatcaacgca ccgatcatgt tgcgcacctg
     4321 atgctgcaaa aaagcattgg cactaaagtt aaattgaaag tacgccttat tttcagttaa
     4381 ttttgtctgc gtcacctcgg cttgatgcat agtacgaaca ggtgaactcg cttgacattc
     4441 agaagcacga aaagcactaa aatcatgctc gccaatcaaa taacccgctg ctttttgcat
     4501 ggccgaataa tcgagcggaa gatgaaacca gcctgacttc ttggccatga gcgctgaggc
     4561 cacgggtgca ttgtagagca gatattcata acggcgactg taggcagaaa accgtgcatg
     4621 aaacgcgctg tcaacttcca ccgcccattc tattcgcacc gtttcgggaa gattcgcatt
     4681 cacgccacgc acccaagcag aaattggccg cgtgctttct gtatcaaaat ggatgacctg
     4741 gcaactggca tgcacacccg tatcagtgcg gcctgctgca tgcactctta cggcatgctg
     4801 tgagatgctg gcaatggctg cttctatggc atcttgcacg ccacaggcag acggctgact
     4861 ttgccagcca cagaagcctg agccatcata ggcaacgcct aatgcgattc tcgtcatata
     4921 ctggcagctt tcacactcaa taacacaagc caaaagaagc acaaggctat ggctactagc
     4981 gcaaatttat cgcgcaattt aaaaggcaaa aaagcaagtt caatttgttt tagcgcattt
     5041 atttcaggca ttaacggttt ttcaaccgca tgcaatctat caaactgctg aacattaaat
     5101 ttgaagttct tatccaacgc cagcgtttca acataatcga gcgtcagtaa taatcgtgcg
     5161 gtaaatcgct ccacattcaa tcctaaccat tgcagtggca acagcaaata atataagcct
     5221 gtcatgagtt gcatttggct acacgttgca aacaatatac ttaaactagc gatcgcaatc
     5281 agcagcttgc ctacttgcaa caggcccgac tgcatgccct cataagtggg cgcagtatca
     5341 aataaaaaag gttgaatata ttcaccaggc gtagcaaacg catacacgat taaaatagac
     5401 aaaaacagcc agcgcattcg caaaacaacc tgactaaaac ttttacgtgc gaagacaaaa
     5461 gcaatcgtgc aactgatcac gcacaacgcg ccaagataaa cttgtgacat gtgactcatg
     5521 ccaatcaata tcatgataaa acttaatatt tttacaaatg ggtgcatcag tgtagggtga
     5581 attatttctt tatacgcgtg gtaatgtttg cgcaaatact aacactttta cctagcaaat
     5641 acagtatgcc aagtatttaa aagtgagcgc aaacactagc ctaaaaaatg gcatttaatg
     5701 cgcattttaa acaccaacct ttttcgatgc tctggaagcc ttataaacaa aggctcacag
     5761 ggcatttatt ttttctcgta tgttttagcg acttgttaat gcttagatat agctaaatat
     5821 ttggtataaa aaaaccgggc aattagcccg gctcttttac gaaacaaata cgcttatgcc
     5881 aaactatcta gcaaggtttg tgcgcgttgt ttttgctgta cactgccttc tttaagcact
     5941 tcttcaagta gctcgcgtgc acctacttta tcatccatat caatataaac tgcgaccaaa
     6001 tcaagtttaa tttcaacgtc ttgtggctca agccctacat tattggctgg tgccagacct
     6061 tctgttgaaa aatcatctac ctgagacgcg gtcggctgca aggtaggagt atctaaatcg
     6121 agattgattg aagacaagtc aaatgtgtgc gcttctggcg cagaaacgat tgaggcgctt
     6181 tcattggcag taattacgtt atccgcctcg gtgtctggca agtcgaatga aaaatctccg
     6241 atagactcca ccgctgcttt ttcttcagcc agattcactg caggcgctga cgctggcgcg
     6301 cttgtttctg ttgaagcagg gaaatcaaaa tccatggcaa aatcaagggc tggcgcttct
     6361 gccgttgtta cctgctcagc attttcaagc gattctgaag taaattctga actcaaatct
     6421 aatgcgggcg catccagcgc atcggttgaa gcagctacag gcaaatcaaa tgaaggcaat
     6481 gagtctgcaa tattttcggt ggctactgaa gcagatgcta ctggttcacc aatatcaaat
     6541 gcgtcaccca agtcaaagtc catgccattg ctaacttctg actgcaatga attttcaatt
     6601 gcatttgcag tttggcttgt atcagtaccg cccaaatcac ctaaatcaaa agtttcagtg
     6661 tcactcgtaa aggcaatttc gttatctgcc ggggcaaagc tgtcggctac cactgaagca
     6721 gcatcttcaa ctgaagcgct ggcttggtca gcaaatgggt cgttcaatac ttcgtcgcta
     6781 tcgagtgaaa aatctaggtt attgtcctct gctttttgcg taacttcatc tgaaccaaaa
     6841 accaaatcgt tttcatcatt cacctgatct aatgtttcgg aagcgccttc tgacaccgcc
     6901 gcaagcttac ttacatcata taaaggatta tctgg
//
LOCUS       DAOQSI010000117         6793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_11066_length_6793_cov_4.612645, whole genome shotgun sequence.
ACCESSION   DAOQSI010000117 DAOQSI010000000
VERSION     DAOQSI010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 6793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6793
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11066_length_6793_cov_4.612645"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PKL42_07750"
     CDS             <1..485
                     /locus_tag="PKL42_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNU67007.1"
                     /translation="GSAEKYVENIVGVIEHIKLFEDFGLDEVKSLCFYMDCYRAPANY
                     TLLEEGAEGDYLMLILSGSVRVEKLIFRQGIREIAEVHAGDSLGEMSLVDGRPRFASC
                     STLETTDFAVLTHDALNEILIHHPRLGNKFLLVLLQLMAARLRDTCDRFLPSVHGAII
                     "
     gene            495..1760
                     /gene="epsL"
                     /locus_tag="PKL42_07755"
     CDS             495..1760
                     /gene="epsL"
                     /locus_tag="PKL42_07755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative exosortase B-associated extracellular
                     polysaccharide biosynthesis transporter EpsL"
                     /protein_id="HNU67008.1"
                     /translation="MMPLTHQTSVQAFKRNLLLPVAHQHLFAGIALLVIGLPFSLFSQ
                     PSIADEEDPLKFIAGVSRLHDNNLFRQNSGKKSENITSAYAGIRLDKLYAQQRFKFDF
                     TLTANRYQNYGILDFNAKDYKAAWLWSLTPFLKGTISLDRKQSLNSFQDFRGGSLVNI
                     RNINTSETQHFEADFSPYNIWHILGGVTRSTQKNSNNSNSFEGQESFTMNSLDAGARY
                     NFSSGSSVTVMGHERSGDYDGRQLNAAALFDTAFDEREVETKLDWILSGKSRVNARVA
                     YLSRDHDHFSQRDFSGLVGNVDYSWTPTGKLRLILSAASSLSSYQTLDNSYTRLNSIS
                     ITPLYAFSDKITMRAKAAITKRTFLGEGVIPDGDRVDWGKTASFGVEWAPLRTISIGG
                     NLERSSRDSNIDVYDYTDTSVGVNANLAI"
     gene            1843..2886
                     /locus_tag="PKL42_07760"
     CDS             1843..2886
                     /locus_tag="PKL42_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03790 family protein"
                     /protein_id="HNU67009.1"
                     /translation="MRSLIFLFFLLSLQGAFAAGNAPSAGGNAEKVSVLGPEHVALVI
                     NDADANSVQIGEYYRLARNIPAKNIVHVNIPKAKAKLSLSEFTRLKWEIDSQLHTEEQ
                     VILLAWTAPYAVECNSITSALTFGYDAEQCKNTCAPGKANPYFNSSAKRPYAELGMRP
                     VMLLPTDSIARAKAVIDRGVQSDAGVFRATAYYLTTSEQARNSRAMYFPPTGLNIPRS
                     GLAVMNIQSDKLVGASDIMIYQTGTAWVEGLETLNFLPGALADHLTSVGGDLQGKGQM
                     SILRWLDAGATASYGTVSEPCNYWQKFPNSAVMLKWYVHGATAIEAYWKSVVWPAQGL
                     FIGEPLAAPFGRQ"
     gene            2891..3565
                     /locus_tag="PKL42_07765"
     CDS             2891..3565
                     /locus_tag="PKL42_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX prenyl protease-related protein"
                     /protein_id="HNU67010.1"
                     /translation="MLNQSVLNQAILARSAPFAFYIIIMMISGLMQDAGVDVRWMYAI
                     RVGGTALLLIYLHKHYAELAWSSKPTAQQLLLAVLAGLVVFLLWISLNQDWMIVGSTS
                     GYDPRTSDGALDIGMVAMRLAGAALIVPVMEELFWRSFIMRWLDLADFQTLAPASVTI
                     KALLISSVVFAIEHTFWLAGLAAGLIYGVLYMRTQNLWVSVIAHAVTNGALGLWVLKT
                     GHWHYW"
     gene            3921..4922
                     /locus_tag="PKL42_07770"
     CDS             3921..4922
                     /locus_tag="PKL42_07770"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsD family peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNU67011.1"
                     /translation="MYQNSAFTSSNSCGNIAKVALFIAVAGLLISCGDKNGATEKAGQ
                     SIVRVNGDEITIHQLNNELQRANVQPAQQDVASKQLTKALVDRQILVQQALKDKLDRN
                     PNVMQAIESAKTQILAQAYLEGKVAAIPAPTATEVNEYRVQHPEIFANRKIYVMEELS
                     FLADASVKSELEALSNTAKTLDEVTQWLDSKQIKYGRANSAHAAESVPKQLLGKFSQM
                     AIGDLIFVNANGRTMVGRMIEVKNVPISEADAKPLIERILVSQKRKEAAEAELTRLRG
                     LAKIEYLNEKFAPSAGTEPAAAQPPATAPVVEDKAASPEIGANDNLDKSIEKGLSGL"
     gene            5003..6037
                     /locus_tag="PKL42_07775"
     CDS             5003..6037
                     /locus_tag="PKL42_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNU67012.1"
                     /translation="MRQIFAYILLAVLGAWLNIASAEEPNYVLGPGDVIKVNVYSNPD
                     LSLEARVSEAGLISYPLIGQVQVGGLTTFAAEKRIAGLLEKRGFIKEPQVNVLVIQFQ
                     SKLVSVLGSVYKPGRYPLERTTNLADLLALVGGATPDGSDLITVVSASGKTEYDLHKI
                     VNQPNGLKNIVFNGGEVVYVHARDVSVMGQVLRPGKYAVVSGVRTVADFLSVAGGVAP
                     SGSDTVTVTTLRDGKISRFEVDVDSLFRTGDNSANIELSSGDSVYVPLAPMVYVYGEV
                     QRPGSFRIARDMTVMQALTLGGGPTIRGTQRNIKLHRRNAKGEIMQLFPALTDAVQQD
                     DVLYVQESLF"
     gene            6047..>6793
                     /locus_tag="PKL42_07780"
     CDS             6047..>6793
                     /locus_tag="PKL42_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HNU67013.1"
                     /translation="MNFTQFLLILKARFWIILATFALVVTTTVVVSLLLPKTYEATTT
                     LVLNYKGMDPVTGVVLPAQLMPGYMATQNDIITSKSVAIKVVDQLGFAKSPAAQAQFN
                     EAAKGKGDIREWFAEKLLKELTVKPSRESSVIDVSFRGADPDFAAAIANAFANAYIQT
                     NLQLKVEPAQKAAAFLDEQTKSLRENLEAAQTKMSKYQQEKGITGGMGSLDVETARLN
                     DLSSQLVMVQSQAFEAESRSKNAQSKGVASP"
BASE COUNT         1845 a         1387 c         1668 g         1893 t
ORIGIN      
        1 tcggcagtgc tgaaaagtac gttgagaaca ttgttggtgt aatcgagcat attaagttgt
       61 tcgaagactt tggtttggat gaagtgaagt ctctttgctt ctatatggac tgctaccgcg
      121 cgcctgcaaa ttacacctta ctcgaagaag gtgcagaggg tgattatttg atgctcattc
      181 tgagcggttc agtccgtgtt gagaagttga tattcaggca aggcattcga gaaattgcag
      241 aagtacacgc gggggattcg ctaggtgaaa tgtcattggt tgatggccgt ccgcgctttg
      301 caagctgctc aacgctagaa acaacggatt ttgcggtgct cacgcacgat gctttaaatg
      361 aaatattgat tcaccatcca cggctaggca acaaattttt attggtgtta ttacaactga
      421 tggcggcgcg tctgcgtgac acttgtgacc gttttctacc tagcgtacat ggtgcgatta
      481 tctaaggtgc tgccatgatg ccacttaccc atcaaacaag tgttcaagct tttaaacgga
      541 atttgttact gcctgtagcg catcaacatt tatttgccgg aattgcgcta cttgtgattg
      601 gtttgccatt ttccttattt tcgcaaccat caatcgccga tgaagaagac ccattgaaat
      661 tcatcgcagg ggtttcacgc ttgcacgata ataatttatt ccgccaaaac tcgggtaaaa
      721 aaagtgagaa catcacgtca gcttatgctg gcatccgttt agacaaactc tatgcgcagc
      781 agcgctttaa atttgatttt acgctgacgg ctaatcgcta tcagaattac ggcattctgg
      841 attttaatgc caaagactat aaagccgctt ggttatggtc gctcacgcct tttttaaaag
      901 gaaccatttc tttagaccgt aagcaaagcc tcaatagttt tcaggacttc agaggcggca
      961 gtttagtaaa tatccgtaat attaacacca gcgaaacgca gcattttgag gcagattttt
     1021 caccgtacaa tatttggcat atcttaggtg gtgtcacacg ctccacgcaa aaaaactcga
     1081 ataacagcaa cagctttgaa gggcaagaaa gttttaccat gaactcgctt gatgcgggtg
     1141 cgaggtataa cttcagttca ggtagctctg taacggtgat gggtcatgag cgaagtggtg
     1201 attatgatgg gcgtcagctc aatgcagctg cgctttttga tacggctttt gatgagcgtg
     1261 aagttgaaac caagcttgat tggatccttt caggaaagtc acgcgtcaac gcccgtgttg
     1321 cctatctttc acgcgatcat gaccactttt ctcagcgcga tttttctggg ttggtgggca
     1381 atgtggatta cagttggaca ccaacaggaa aattacgcct aatcttatcg gccgcgagtt
     1441 cgctttcaag ttaccagact ttagacaata gctacacacg cttaaattct ataagcataa
     1501 cgccactgta tgctttcagc gataaaatta ccatgcgtgc aaaagccgct attaccaagc
     1561 ggacttttct tggtgaaggg gtcattccgg atggcgatcg cgttgattgg ggaaaaaccg
     1621 caagttttgg tgtggaatgg gcgccattac gcaccatcag tatcggcggt aacttagagc
     1681 gtagcagccg cgattcaaat attgatgtgt atgattacac cgacacaagt gtgggtgtaa
     1741 atgctaattt agcgatttaa tctattctct gcgtttaaac ttgcagcgaa atgctgggtc
     1801 acagaacttc taatctacgc tgatattcac aagctgaccg cgatgcgctc ccttatcttt
     1861 ttattttttt tattgagcct gcaaggggca tttgcggcag gcaatgcacc aagcgcaggt
     1921 ggcaatgccg agaaagtaag cgttcttggc cctgagcatg tggcactggt tattaacgat
     1981 gcggatgcca atagcgtaca aatcggtgaa tattatcgtc tcgcccgcaa tattccagct
     2041 aaaaatattg tacatgtgaa tattcccaag gctaaagcca agttaagttt aagcgagttt
     2101 actcgcttaa aatgggaaat agattcgcaa ttacatactg aagaacaagt cattttactg
     2161 gcgtggacag cgccttatgc tgtggaatgt aattcaatca cttccgcact gacttttggc
     2221 tacgacgcag agcagtgcaa aaacacctgc gcaccaggca aggcaaaccc ttattttaat
     2281 tcaagcgcaa aaaggccgta tgctgagcta ggcatgcgcc ccgtcatgct gttacctaca
     2341 gattctattg cgcgtgccaa agcggtgatt gaccgtggtg tacaaagcga tgcgggcgta
     2401 tttcgcgcaa ccgcctatta cctcacaact tctgagcaag cccgcaatag ccgtgctatg
     2461 tattttccgc caacggggtt aaatattccc cgaagtggtt tggctgtcat gaatattcag
     2521 tcggataagt tagtaggtgc cagtgacatt atgatttacc aaacaggcac agcttgggtt
     2581 gagggcttgg agacgctcaa ttttttaccg ggtgcgctgg ctgaccactt aacttctgtt
     2641 gggggtgatt tacaaggcaa agggcaaatg agcattctgc gctggctgga tgccggggcc
     2701 acggcaagct acggcacggt ttctgagccc tgcaactatt ggcaaaaatt tccaaactct
     2761 gcggtgatgc ttaaatggta tgtgcatggt gcaacagcaa ttgaagccta ttggaaaagt
     2821 gttgtctggc ctgcacaggg gctatttatt ggtgagccat tagccgcacc ctttggcagg
     2881 caataacata atgttgaatc agtcggtctt gaatcaggca atattggcaa ggtctgcacc
     2941 gtttgctttt tatatcatca tcatgatgat ttcaggcttg atgcaagatg ctggcgtaga
     3001 tgtgcgctgg atgtatgcga tacgggtggg tggcacagcc ttactgttaa tttatttgca
     3061 caagcactat gcagaactcg catggtcaag caagccaact gcgcagcaat tattgttagc
     3121 agtgttggct ggcctggtgg tgtttctgct atggattagt ttgaatcagg attggatgat
     3181 tgttggttca acttcaggct acgatccgcg aaccagcgat ggtgcgctgg atatcggcat
     3241 ggtggccatg cgtttggcag gcgcagcttt gatagtgccg gtcatggaag agcttttttg
     3301 gaggtcattc atcatgcgtt ggctagacct tgctgatttt caaacgctcg cgcctgcaag
     3361 tgtgactatt aaggcgctgt tgatatcttc agtggtgttt gccattgaac atactttttg
     3421 gttggcaggg ttagcggcag gcttaattta cggtgtgttg tacatgcgca cgcaaaatct
     3481 gtgggtgagc gtgattgcac acgcagtcac caatggcgca cttgggcttt gggtgcttaa
     3541 aactggccat tggcattact ggtaaatcgt cgttaaaacc agcattttga tatgaaacct
     3601 ttttggctgc cctgcgggcc tttatttgca aggcgcgcag agcagaataa ttctctcgtg
     3661 tgaaatatgc attttattta agtgtgatta gcgatgatgt tctgtgatat ttatatctaa
     3721 gattattcgc tgaaataatt ttaactgttg gtaattgtaa ttttttttga ttaaaattaa
     3781 gtctgttaaa tcaattaaat agcctgatat tactatttac ttctttgcct ttatagggtg
     3841 ataatacagc gagactatgg taagtttagc caaaattggt tttctaaagc gttattcgag
     3901 caaattttta aggaacatcc atgtaccaaa attcagcatt cacatcatca aattcatgcg
     3961 gcaatattgc taaagttgca ctgtttattg cagtcgcagg cttacttatt tcatgtggtg
     4021 ataaaaatgg cgcaacagaa aaagcgggcc aatcgattgt gcgtgtgaat ggtgatgaaa
     4081 ttaccattca tcaattgaat aatgaattac agcgtgcaaa tgttcagcct gcacagcagg
     4141 atgttgcaag taaacaactc actaaagcct tggttgatcg ccaaattctt gtgcaacagg
     4201 cgctaaaaga taagctcgac cgtaacccta acgtgatgca ggccatagaa agtgcaaaaa
     4261 cgcaaatact ggcgcaagct tatttagaag gtaaagtggc cgcaattcct gcgccaacag
     4321 caacagaagt caatgaatac cgtgtgcagc accctgaaat ttttgccaat cgtaaaattt
     4381 atgtcatgga agaattatca tttttggcgg acgcaagcgt taagtctgaa ttggaagcgt
     4441 taagtaatac ggctaaaaca ctggatgaag ttacccaatg gttggactca aaacaaatta
     4501 agtatggacg ggcaaattca gcacatgccg ctgaatctgt gccaaaacaa ctgttaggta
     4561 aatttagcca aatggccatt ggtgatttaa tttttgtaaa tgcgaatggc cgtaccatgg
     4621 tagggcgcat gatagaagta aaaaatgtac caatttcaga agccgatgct aaaccgctga
     4681 ttgagcggat tctcgtttca caaaaacgta aagaggcggc agaggctgaa ctcacacgtt
     4741 tacgtgggtt agctaaaatc gagtatctga acgagaagtt tgccccatca gccggcactg
     4801 agcctgccgc agcacagccg ccagcgacag cgccagtggt ggaagataag gccgccagcc
     4861 cagaaatagg cgccaatgat aatcttgata aaagcataga aaaaggctta tcagggctct
     4921 aagctggttg aaaggatagt tggtgactta gtttgccctg cttttctgtt aaaatacaca
     4981 tctaacaagt tttaggaaaa atatgcgaca aattttcgct tacattttat tggcagtgct
     5041 tggtgcttgg ttaaatattg cctcagctga agagcctaac tacgttttgg gtcctggtga
     5101 tgtgattaaa gtcaatgttt acagcaaccc agatttatcg cttgaggcac gcgtttcaga
     5161 ggcaggttta atcagttacc cgcttattgg tcaggtgcag gtaggcggct taactacgtt
     5221 tgctgctgaa aaacgtattg ctggcttgtt agaaaaaaga ggctttatta aagaaccgca
     5281 agtcaatgta ctggtgatac agtttcaaag taaattagtt tctgtacttg gcagtgtgta
     5341 taagcctggt cgttatccgc ttgaacgcac cacgaatttg gctgatttac tggcgcttgt
     5401 tggcggtgcc acgccagatg gctctgattt gattacggta gtcagtgctt ctggtaaaac
     5461 tgagtacgat ctacacaaaa tcgtcaatca acctaacggc ttaaaaaaca tcgtttttaa
     5521 tggtggtgaa gtggtttacg tgcatgcgcg cgatgtgtcc gtaatgggcc aagtgctgcg
     5581 cccaggtaag tacgctgtag tgagcggtgt gcgcacggtt gctgattttc tatcagtggc
     5641 aggcggtgtt gcgccgagcg gctccgatac ggtgacggtc acgactttac gtgatggaaa
     5701 aatcagccgc tttgaagtgg atgtcgatag tttattccgc acgggcgata acagtgccaa
     5761 tattgagtta tcaagtggcg atagcgttta tgtgccgctt gcacccatgg tgtacgtgta
     5821 cggtgaggtg cagcgcccag gttcatttcg tattgcacgt gatatgacgg taatgcaagc
     5881 gcttacgcta ggtggcgggc caaccattcg cggtacgcaa cgcaatatta aactgcaccg
     5941 ccgcaatgcc aaaggtgaga ttatgcaatt attccctgcg ctgacagatg ctgtgcagca
     6001 ggatgacgta ctttatgtac aagaaagcct gttctaggaa attaaaatga atttcacgca
     6061 gtttttgctt attcttaaag cgcggttttg gattatattg gctacattcg ccttggttgt
     6121 aacgacaacc gttgtagtca gcttgttgtt accaaaaact tacgaagcaa ccaccacatt
     6181 agtactcaat tataagggca tggaccccgt cacgggggta gtgttgcccg ctcaactaat
     6241 gcctggctac atggcaacac aaaacgacat tattaccagt aaaagtgttg caattaaggt
     6301 ggttgatcaa ctaggatttg ccaaaagccc ggcagcacaa gcacagttta atgaagcggc
     6361 aaaaggtaaa ggcgatatac gcgaatggtt tgctgagaag ttgttgaaag aacttaccgt
     6421 taaaccatca cgtgaaagta gtgtgattga tgtttcattt agaggggctg accctgactt
     6481 tgcagcggcc attgccaatg cctttgccaa tgcttatata caaactaatt tacagcttaa
     6541 agtagagcca gcgcaaaagg cggcagcttt tttagatgag caaacaaaat cactccgcga
     6601 aaacctggaa gccgctcaaa ctaaaatgtc aaaatatcag caagaaaaag gcattacagg
     6661 cggtatgggc agtttagatg tagaaactgc aaggttaaat gatttgtcat cgcaattggt
     6721 gatggtgcaa tcacaggctt ttgaggcaga gtcccgttca aaaaatgcgc agagcaaggg
     6781 tgttgcgtca cct
//
LOCUS       DAOQSI010000118         6745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_11180_length_6745_cov_5.639312, whole genome shotgun sequence.
ACCESSION   DAOQSI010000118 DAOQSI010000000
VERSION     DAOQSI010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 6745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6745
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11180_length_6745_cov_5.639312"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PKL42_07785"
     CDS             <1..195
                     /locus_tag="PKL42_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67014.1"
                     /translation="HKNAREKFYALEAFIYKASRASKKVALKKECFFVVLLNCLPPKL
                     RKKLLAKFGLKNHNYQIISS"
     gene            261..627
                     /gene="ssrA"
                     /locus_tag="PKL42_07790"
     tmRNA           261..627
                     /gene="ssrA"
                     /locus_tag="PKL42_07790"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            778..1668
                     /gene="rfbD"
                     /locus_tag="PKL42_07795"
     CDS             778..1668
                     /gene="rfbD"
                     /locus_tag="PKL42_07795"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNU67015.1"
                     /translation="MKILLFGKNGQVGWELQRSLVPLGELIALDARSEILCGDLTNLA
                     GIANTIRTVAPDIIVNAAAHTAVDKAESEPELANTLNAQAPEAMAKEAARLGAWLVHY
                     STDYVFDGSGETPWRETDTTGPLSIYGRSKLEGELAIIASGCKHLIFRTSWVYAARGG
                     NFAKTMLKLAKERDSLKVIYDQIGAPTGADLLADVTAHAIRTASRQVEVSGLYHLVAG
                     GETSWHGYASFVIEFARQAGVEIKVPTDAIGPVPTTAFPTPATRPLNSRLDTQKLQNT
                     FKLYMPHWQTGVTRMLTEIL"
     gene            1754..2641
                     /gene="rfbA"
                     /locus_tag="PKL42_07800"
     CDS             1754..2641
                     /gene="rfbA"
                     /locus_tag="PKL42_07800"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNU67016.1"
                     /translation="MTQRKGIILAGGSGTRLHPVTKVVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSDWGLNLQYAVQPSPDGLAQAFIIGKDFIGN
                     NPSALVLGDNIFYGHDLHKQLKQATLRNEGATVFAYHVNDPERYGVVDFNKQGHATSL
                     EEKPAKPKSNYAVTGLYFYDNQVAKLAANLRPSPRGELEITDLNRIYLERNELNVEIM
                     GRGYAWLDTGTHESLIEASNFIQTIEHRQGLKVCCPEEIAYRNGLISAEQLEKLAQPL
                     AKNGYGQYLLRLLKEGMPF"
     gene            2638..3183
                     /gene="rfbC"
                     /locus_tag="PKL42_07805"
     CDS             2638..3183
                     /gene="rfbC"
                     /locus_tag="PKL42_07805"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNU67017.1"
                     /translation="MKAIATSIPDVLIIEPKVFGDERGFFFESFNRNQFTELIGREVY
                     FCQDNHSRSAKNVLRGLHYQIQNPQGKLVRVVQGAVLDVAVDIRKSSPTFGQHVAFEL
                     SAENKRMMWVPEGFAHGFLVLTESADFLYKTTDYYAPSFERSIIWNDPEIGIQWPSSA
                     APILSAKDQQAKLLADAEVFA"
     gene            3315..3626
                     /locus_tag="PKL42_07810"
     CDS             3315..3626
                     /locus_tag="PKL42_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HNU67018.1"
                     /translation="MAQFDNVSVKKKANIYFDGKCVSHTVMLPNGTRSTIGVIFPSAL
                     TFNTAAPELMEINSGHCKVRLNGETAWKAYGAGEKFTVPGNSSFDIEVTETLDYVCHF
                     E"
     gene            3732..4226
                     /locus_tag="PKL42_07815"
     CDS             3732..4226
                     /locus_tag="PKL42_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HNU67019.1"
                     /translation="MPSFDISSEVDMVALKNAVDTASKQITNRYDFKGTAAKIELNEK
                     DNLITLLGDSDFQLDQVKDILLPAMEKKEPDSSKRLDHQDVQKVGGNKVKQELKIKAG
                     IDTELAKKLVKLLKDSGLKVQASIQGDTVRVNGAKRDVLQEAIAFCKKSVTDFPLQFG
                     NFRD"
     gene            4262..5848
                     /gene="cimA"
                     /locus_tag="PKL42_07820"
     CDS             4262..5848
                     /gene="cimA"
                     /locus_tag="PKL42_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HNU67020.1"
                     /translation="MQTKVTAYDSTLRDGAQAQGVSFTVEDKLKIVAQLDALGVGYIE
                     AGNPGSNPKDLEFFKRAAELKLQHAKIIAFGSTRRIGIQAADDNNLRSLLSANTQAVA
                     IFGKSWDYQVTDILRTTLAENLAMIADTIAYLKQQGKEVVFDAEHFYDGYKANAEYAF
                     ATLKAASDAGADVLVLCDTNGGTFPNEVFEITRKVVSQFSAAQSGGVQVGIHCHNDCE
                     MAVANSVAAVQAGATQVQGTINGIGERCGNANLCSIIPNLQLKLGLSCIPPENISSLT
                     HVARFVNEVANMPFNDKAPYVGNDAFSHKGGMHIDAVNKNPISYEHINPEQVGNIRHI
                     LVSEVAGRGALLSKLHEIDATLDKDSADTQRILARIKALEHDGYQFESAEASVQLLVL
                     RELGKLRPTFEFKEYQVNIHEPAINGVNSSARIKLVVNGQEEIMSAEGDGPVNALDKA
                     MRRALERFYPTIQSVRLTDYKVRVLNSDQATAAKVRVLIESADHNESWTTTGVSTDVV
                     DASWRALIDAIEYKLMRDLV"
     gene            complement(5937..6296)
                     /locus_tag="PKL42_07825"
     CDS             complement(5937..6296)
                     /locus_tag="PKL42_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67021.1"
                     /translation="MGHKIAIIILADTDTNDGLGRVVNALAAAGEYDKAGDEVRIVFT
                     GTGTKWPTSLEKSDHPAHGLYVAAKGLIDGACGFCANAFGQTEAMKNYSIKLLETSGP
                     FMSYRQFTEEDFQILTF"
     gene            6427..>6745
                     /locus_tag="PKL42_07830"
     CDS             6427..>6745
                     /locus_tag="PKL42_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNU67022.1"
                     /translation="MTDRLEALFNQHALNARMIFSGTLCNTTLFSEDANRGFLHLVRK
                     GKLIVYSPTHAPISIEQPSLLLYPSAVKHQFVFDGDVDLTCASIDLDGGSGVLANALP
                     AVLL"
BASE COUNT         1900 a         1413 c         1585 g         1847 t
ORIGIN      
        1 cataaaaatg cccgagaaaa attttatgct ctggaagcct ttatttataa ggcttccaga
       61 gcatcgaaaa aggttgcgct taaaaaggag tgtttttttg ttgttttatt aaactgttta
      121 ccgcctaaat tacgcaagaa attgttggct aaatttgggt taaaaaatca caactatcaa
      181 attatctcat cataaaagcg tataattcgc acattgatgc aactaacttc aagtaaagtg
      241 cgtcatagca agactgattt ggggctgacc aggtttcgac gtgggttaca aagcagtgca
      301 gggcataccg agggttcagg tttcctcgta aatccatctg aaaaagtata gtcgcaaacg
      361 acgaaactta ctctctagca gcttagttct gccggacgct acaccagctc tcccgtgggt
      421 tgggttgggg taacccatac gtagtgtcat atacacggga tacgtgttgt gttgggtgac
      481 ttagcacatc attaacctta aggtctctcg tctgcacact gcttgtctgt tggtgtggtg
      541 cagattaaat taaacaacaa ggctaagtat gtagaactgt ctgtagcgga tttgcggacg
      601 ggggttcgat tccccccagc tccaccaaac aaataatgcc acccttgagg tggtattttt
      661 gtttatactt atcaggtgtt agcgcgccaa aagagctatt gtcaggcgat ttctcaacgt
      721 gatataacgt cacacaatcg acatcatcat gataaaaata agatttaacc cgttgctatg
      781 aaaattcttt tgtttggtaa aaatggtcaa gtgggctggg aattacagcg tagccttgtg
      841 ccgttaggcg agttaattgc actggatgca cgcagtgaga ttttgtgtgg tgacttgacc
      901 aatttggcgg gtattgccaa cactatccga acggttgcgc ctgatattat tgtgaatgcc
      961 gccgcccata ctgcggtcga taaggccgaa agtgaacctg agttggcaaa cacgcttaac
     1021 gcccaagcac cagaagccat ggcgaaagag gcggcacgcc ttggggcctg gttggtgcat
     1081 tactctacag attatgtgtt cgatggctct ggcgaaacgc cttggcgcga aaccgataca
     1141 actgggcctt taagcattta cggtagaagc aaattagaag gtgagttggc cattattgca
     1201 tcaggttgca agcatttgat ttttcgcacc agttgggtgt atgcagcgcg tggcggaaat
     1261 tttgccaaaa cgatgttaaa gcttgcaaaa gagcgtgata gtttgaaagt aatttacgac
     1321 cagattggtg cgccaacagg cgctgatttg ctggcggatg tcactgccca tgcgattcgc
     1381 actgcatcac ggcaggttga agtaagcggt ttatatcacc ttgtggcagg tggcgagact
     1441 tcatggcatg gctatgcgag ttttgttatc gagttcgcgc gtcaagcggg cgtggagata
     1501 aaagtaccta ccgatgcgat tgggcccgtg cctaccacgg cttttcctac accggcaacc
     1561 cggcctctga attcgcgctt ggatacgcag aagctgcaaa atacgtttaa attatatatg
     1621 ccacattggc agaccggtgt tacgcgtatg ctgaccgaaa ttctttagca cacctgtttt
     1681 gaaattaaca tttggtaaaa ataaatcaaa ttaactgcta aaattactcc aacatattga
     1741 tagagaaaca attatgacgc aacgcaaagg cattatcctt gcgggtggct ctggcacccg
     1801 acttcaccca gtgacgaaag tcgtttcaaa acagctactg cctatttacg ataaaccaat
     1861 gatttattat ccgctcagca cattaatgct ggcaggtatt cgcgatattt tgattatctc
     1921 aaccccgcaa gatacgccgc gatttcagca attgctgggc gatggcagcg attgggggtt
     1981 gaacctgcaa tatgcagtac aaccaagtcc agatggtctg gcgcaagcat ttattatcgg
     2041 caaagatttt atcggcaata atccaagcgc attagtgttg ggcgacaata ttttttacgg
     2101 tcacgattta cataagcaac ttaagcaagc gaccttacgt aatgaggggg ctacagtttt
     2161 tgcctatcat gtgaatgacc ctgagcgcta tggcgttgtt gacttcaaca agcaaggaca
     2221 cgcaaccagt ttggaagaga aacctgcaaa accaaaaagc aattatgcgg tgacggggct
     2281 gtatttctat gacaatcaag tggctaaact agcagcaaac ctcagacctt caccccgtgg
     2341 ggagttggaa attaccgatt tgaatcgtat ttatcttgag cgcaatgaat tgaatgtgga
     2401 aatcatgggc cgtggctatg cgtggttaga taccggcacg catgaaagcc tgattgaagc
     2461 cagtaatttt attcaaacga ttgagcatcg gcaaggctta aaagtctgct gcccggaaga
     2521 gattgcttat cgcaatggct tgattagtgc cgaacaatta gaaaagttag cacaaccctt
     2581 ggctaaaaat ggctatgggc aatatttatt acgtctgtta aaagaaggga tgccgttttg
     2641 aaagccatcg ccacttcgat tcctgatgtg ttgattattg aaccaaaagt gttcggtgac
     2701 gaacgcggct tcttttttga aagttttaac cgcaatcagt ttaccgagct aattggtcgt
     2761 gaggtgtatt tctgccagga taatcacagc cgttcagcca aaaacgtttt gcgaggattg
     2821 cactatcaaa tacagaaccc gcaaggtaaa ttggtgcgtg tggtgcaggg tgcagtgttg
     2881 gatgtggcgg tggacattcg caaaagttca ccgacctttg ggcaacatgt ggcctttgaa
     2941 ctgtcggcag aaaataagcg catgatgtgg gtacctgaag ggttcgcgca tggtttcttg
     3001 gtgcttactg agtctgctga ttttttatat aaaacaacgg attactacgc gccatcgttt
     3061 gagcgcagca tcatttggaa cgacccggaa attggtattc aatggccaag tagcgccgcg
     3121 ccgattcttt cagccaaaga ccaacaggca aaattattgg cagacgctga agtgtttgct
     3181 taatttctct cgcaatcact caatctatcg acttaacttt catcatgttt aaggtttaaa
     3241 gcgcaagcac cgtcttggat aggcggtgct tttctgcgat aatggcgttt tgattccttt
     3301 tgaaagtatg cgccatggca caattcgaca acgtcagtgt aaaaaagaaa gccaatattt
     3361 atttcgatgg taaatgtgtg agtcacacgg tgatgttgcc aaatggcacg cgtagcacca
     3421 ttggcgtcat ttttccaagc gcactcacgt ttaatactgc agcccctgaa ttgatggaaa
     3481 tcaacagtgg ccactgtaag gtgcgcctca atggcgaaac cgcttggaaa gcatatggcg
     3541 cgggtgaaaa gtttaccgtg ccgggcaact cttcattcga tattgaagtg actgaaacgc
     3601 tcgattacgt atgtcatttt gagtagaaaa tacgctcaac ctttttcgat gccctgcgag
     3661 ccttataaat aaaggcttcc agaggcgaaa atttttctcg gacgcttttc acataattta
     3721 aaagggtttt tatgccttca tttgatatca gttctgaagt cgatatggtc gcgttaaaaa
     3781 acgcggtgga tactgccagc aagcaaatta ccaatcgcta tgattttaaa ggcactgcgg
     3841 caaaaataga gctgaacgaa aaagacaacc tgattacttt attgggcgac agcgattttc
     3901 agcttgacca agttaaagat attttattac cggcgatgga aaaaaaagag cccgactcat
     3961 ctaaacggct tgaccatcaa gatgtgcaaa aagtgggcgg caataaagta aagcaagagc
     4021 tcaaaattaa agcgggcatc gatacggagt tggcaaaaaa acttgtcaaa ctgctcaaag
     4081 attctggctt gaaagtgcag gccagcattc agggtgatac ggtgcgtgtc aatggtgcta
     4141 agcgcgatgt gctacaagag gccattgcct tttgtaagaa aagtgtgacg gattttccgt
     4201 tacagtttgg taattttaga gattaatcat cccaccctct cagaacaatt aagaacaatc
     4261 gatgcaaact aaagtcacgg cttacgattc tactctgcgc gacggcgcac aagcgcaagg
     4321 cgtttcgttt acggttgaag ataaacttaa aattgttgcg caattagatg cattaggcgt
     4381 tggctacatt gaagccggca acccaggctc taaccctaaa gatctggaat tttttaagcg
     4441 cgctgccgag cttaaattgc aacacgccaa aatcattgct tttggcagca cgcgccgcat
     4501 cggcatccaa gcggcagacg acaacaatct acgctctttg ctaagcgcca atacgcaagc
     4561 ggtggcaatt tttggcaaaa gttgggatta ccaagtcacc gatattttgc gcaccacgct
     4621 agcagaaaac ctagccatga ttgctgacac cattgcttat ttaaagcaac aaggcaaaga
     4681 ggtggtgttt gatgccgagc atttttatga tggctataaa gccaatgcag aatatgcttt
     4741 tgctacgcta aaagctgcat ctgacgcagg cgcggatgtg ctggtgttgt gtgacaccaa
     4801 tggcggcact ttccctaacg aagtttttga aatcacgcga aaagtcgttt cccaatttag
     4861 cgctgctcaa tcaggcggtg tgcaagttgg catccactgt cataacgatt gtgaaatggc
     4921 ggtggcgaat tctgttgccg ccgtgcaagc aggggctacc caagtgcaag gcaccatcaa
     4981 cggcattggc gagcgttgtg gtaatgccaa tttatgttcc atcatcccca atttgcaatt
     5041 aaagctcggg ctgagttgca ttccaccaga aaatattagc agcctcacgc atgtggcgcg
     5101 ttttgttaat gaagtcgcta atatgccgtt taatgacaaa gcgccatatg ttggaaacga
     5161 tgcgttctcg cacaagggcg gaatgcatat agatgcagtc aataaaaacc ctatttctta
     5221 tgagcatatc aacccagaac aggtaggtaa tatccgccat attttggtgt ctgaggtagc
     5281 ggggcgcgga gcactacttt ctaaactgca cgaaattgat gctacgctag ataaagactc
     5341 ggctgatacg caacgcattt tggcaaggat taaagccctt gagcatgatg gctatcagtt
     5401 tgaaagtgca gaagcttcag tgcagttgtt ggttttgcgc gagttgggta agttgcggcc
     5461 aacttttgaa tttaaggaat atcaagtcaa tattcatgag cctgctatca acggcgttaa
     5521 ttcatcagcc cgaataaaat tggtagtcaa tgggcaagaa gaaatcatgt cggcagaagg
     5581 cgatggccca gtcaatgcat tagataaagc tatgcgtcgt gcactagagc gcttttaccc
     5641 tacaattcaa agtgtgcgtc tcacagatta caaagtgcgc gtgttgaact cagaccaagc
     5701 caccgccgct aaagtacgcg tgttaataga atccgccgac cacaatgaaa gctggacaac
     5761 cacaggtgtt tcaaccgatg tggtcgatgc ttcatggcgc gcgttgattg atgcgattga
     5821 gtataaattg atgcgggatt tggtttgata ttttgttgga tatatggatt tattgttagg
     5881 aagcaatgta ggttgtgcaa cataacgctt tttgcacacc ctacgctggc tagttattaa
     5941 aaagttaaga tttgaaaatc ttcttcagta aattggcggt agctcataaa tgggccgcta
     6001 gtttctagca atttaatcga ataatttttc attgcttcgg tttgcccaaa agcatttgcg
     6061 caaaagccac atgcaccatc aatcaagcct tttgcagcaa catataagcc atgtgcaggg
     6121 tgatcagact tttctaggct ggtaggccat ttagtgcctg tgccagtaaa cacaatacgt
     6181 acttcatcac ctgctttgtc atactcgcct gctgctgcaa gcgcgtttac aacgcgacct
     6241 aaaccatcgt tagtatctgt atctgctaat ataataattg ctattttatg acccattttt
     6301 aaatctcctt aaatttatga cgaataatac tgaactcaaa ctacaagaca cattttaggc
     6361 aagtaataat acttatgata ttatttagtc tcatttaatt accaattagt atcaaatagc
     6421 taaaacatga cagacagatt agaagcattg tttaatcagc atgcgctaaa tgcacgtatg
     6481 attttttctg gtactttatg caataccacg ctgtttagtg aagatgctaa tcgtggattt
     6541 ttgcatcttg tgcgtaaagg caagttaata gtttattcgc ccacacacgc gccaatttct
     6601 atagagcaac ccagtctgct gttataccca agcgcggtaa aacaccaatt tgtttttgat
     6661 ggcgatgtcg atttaacctg tgcaagtatc gatttagatg gcggttctgg tgtcttggca
     6721 aatgccttac ctgccgtgct gctga
//
LOCUS       DAOQSI010000119         6601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_11539_length_6601_cov_4.072716, whole genome shotgun sequence.
ACCESSION   DAOQSI010000119 DAOQSI010000000
VERSION     DAOQSI010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11539_length_6601_cov_4.072716"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..844
                     /locus_tag="PKL42_07835"
     CDS             98..844
                     /locus_tag="PKL42_07835"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na-translocating system protein MpsB"
                     /protein_id="HNU67023.1"
                     /translation="MRYSSEAKERSRRFASINTRAPISKVREAIKARSMSLFEPRPEL
                     GHGSNTLCIVGKRALTKYMMLDRRAFMNSYDYKTDLDGQLLTNIMKPLGPVCGGINLE
                     YYFSRVDNDKLGAGTKLPHNVVGLFGVANSSDGDLRPGLPWQMVEVHDPIRLLIIVEH
                     FPEVVLNAVQSTPEMYEWFINEWVHLVAVNPETHTLHYFKQGKFVPYETLATTLPSIG
                     DFNQLMEDTPPMRTNQLPDATKENLAVYLN"
     gene            857..2725
                     /locus_tag="PKL42_07840"
     CDS             857..2725
                     /locus_tag="PKL42_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNU67024.1"
                     /translation="MQSLLPLYVFIPLIGFFLSLCFERTQEKAMSRIAITTVSLHLLL
                     VVVGLVGWLSHGMTTQHFKYWQLFEHEGFKFALDFYLDKITIVFALVGSIITLLTLIF
                     SKFYMHRDEGFKRFFCTLLLFYAGFNFAVFSGNFETLFIGWEMLGFTSFLLIGYYRNR
                     YLPVKNAFKVLSLYRLGDVCLILTMWMAHHVFHTNITFIELSSNNWQEAFHIAEKPGS
                     LLFMLSMVLVAVAVKSAQFPFSSWLPRAMEGPTTSSAIFYGALSSHLGIFMLLRTYPL
                     WQAVPYFNYAIIVLGLITTVTAMLTVRVQSTIKTQIAYGTVTQLGIMLIEIGLGWHTL
                     ALVHFAGHAFLRAYQLLVSPSSLGYMIHNQFFEYQAKPINHMHVTSRLRNTFYMLSVK
                     EWNLDALQQNYLWRPFKWLGKKFVWLESLASQVALTLFIVLGLMTELNYVSLPQAVRA
                     YFPELYAALSLMCILAAFSSRGNPMRAWWKIIMSQFFIVLGVSLNVHVEAAHVWIYLT
                     TTISAALVGMYCLHNTYHLDKDINLNQYHGYVQERPDLALIFLISCMALVGFPFTPAF
                     IAIDLMFTQIHAHQYVLVSLTGFGFIFLELAAIRIYAKVFLGIHKKHTHPTAYRSS"
     gene            complement(2848..3201)
                     /gene="fdx"
                     /locus_tag="PKL42_07845"
     CDS             complement(2848..3201)
                     /gene="fdx"
                     /locus_tag="PKL42_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HNU67025.1"
                     /translation="MPQIIVLPHIELCPEGTAFEAEPGVSICDNLLNHDIDIDHACDK
                     VCACTTCHVIVREGYNTLNAAQDNEEDLLDKAWGLEPNSRLSCQAIVGKDDLVVEIPK
                     YSINMAKEGHVKQKN"
     gene            complement(3240..5147)
                     /gene="hscA"
                     /locus_tag="PKL42_07850"
     CDS             complement(3240..5147)
                     /gene="hscA"
                     /locus_tag="PKL42_07850"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HNU67026.1"
                     /translation="MALLQISEPGQSAAPHQHKLAVGIDLGTTNSLVATVRSGMSTVL
                     QDEYGHALLPSVVRYLPNGEIVVGHEAQAQQSADPMNTIVSAKRFMGRSLHDIADRAH
                     IPYRFVEDAASNKQVQLHTRAGLKTPVEISAEILKALKVRAEHSLGGELTGAVITVPA
                     YFDDAQRQATKDAARLAGLNVLRLLNEPTAAAVAYGLDNNAEGTFVIYDLGGGTFDVS
                     ILKLTKGVFEVLATNGDSALGGDDFDHRIYCWILDKVRETSNNFSPPQEFKPLGEEDT
                     RLLLTKARQAKEWLTDNFEAQIVCKLSNGILVDVTLSATQFVELTQHLVTKTLTPTRK
                     AMRDAGLDIADIKGVVMVGGATRMPQVRHAVEDYFKQSPLTNLDPDKVVALGAAIQAN
                     ALVGNRSEHDLLLLDVTPLSLGLETMGGLVEKIIPRNSTLPVARAQDFTTYKDGQTAM
                     SIQVVQGERELVADCRSLARFELRGIPPMAAGAARIRVTFQVDADGLLSVTAREQTSG
                     VEAHVVVKPSYGLTEDEITRMLTSSFGSAEADKKARMLAEARVNAGAIINAVTLALAA
                     DSHLINDEDKQAIEAEIARLQSMTSAEDAVAINKGVEALNHATEAFAQARMNASVTHA
                     LSGKNLEDINL"
     gene            complement(5228..5746)
                     /gene="hscB"
                     /locus_tag="PKL42_07855"
     CDS             complement(5228..5746)
                     /gene="hscB"
                     /locus_tag="PKL42_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HNU67027.1"
                     /translation="MSVSYYAFFGLDARFELDLQALENSYRKIQSAHHPDRFVTATAS
                     DRLQSMQVATQANEAYLTLKKPAARAKYLLELQGIQAISETNTAMPIDFLMQQMEWRE
                     QLEDAKQEKNIEALEALIRTLKTESNRLEADFSDQYDLKKDYTSATDTARKLIFIDKL
                     SSDIRTAVELLD"
     gene            complement(5743..6066)
                     /gene="iscA"
                     /locus_tag="PKL42_07860"
     CDS             complement(5743..6066)
                     /gene="iscA"
                     /locus_tag="PKL42_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017904917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HNU67028.1"
                     /translation="MAITLTQTAASRVEKFLSNRGKGIGLRLGVKTTGCSGMAYTLEF
                     VDEMHPEDMAFESHGVKVIVDPKSLVYIDGTELDFTKEGLNEGFKFNNPNVKDECGCG
                     ESFTV"
     gene            complement(6197..6589)
                     /gene="iscU"
                     /locus_tag="PKL42_07865"
     CDS             complement(6197..6589)
                     /gene="iscU"
                     /locus_tag="PKL42_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HNU67029.1"
                     /translation="MAYSDKVLDHYENPRNVGSLDKNDPQVGTGMVGAPACGDVMKLM
                     IKVNDDGIIEDAKFKTYGCGSAIASSSLVTEWLKGRTIDEAYAIKNSEIAEELALPPV
                     KIHCSVLAEDAIKAAVADIKAKQAAKEA"
BASE COUNT         1615 a         1474 c         1479 g         2033 t
ORIGIN      
        1 aatccacgtg aggaggattc gatttccttc gcttccacaa attacactgg aaggtgtaat
       61 ctaacaccat aataaagttc gaacataaaa atggtgtttg cgatatagct cagaggcaaa
      121 agagcgctct cgccgatttg catcaattaa cactcgagcg ccaatctcta aggtacgcga
      181 ggccattaaa gcgcgttcta tgtctctgtt tgaaccacgt ccagagttgg gccatggtag
      241 caatacgctg tgcattgtgg gtaagcgtgc gctgactaaa tacatgatgc tcgatagacg
      301 cgctttcatg aactcgtacg attataaaac ggatttagac ggtcagttac tgactaatat
      361 tatgaagccc ctaggacccg tgtgtggcgg tattaatttg gagtattatt tttcacgcgt
      421 agataacgat aaactcggag caggcacaaa gttgccgcat aatgttgttg gtttgtttgg
      481 tgttgccaat agtagtgatg gtgatttacg accaggtctg ccttggcaaa tggtagaagt
      541 tcacgaccct atccgtctgt tgattattgt ggagcatttc cctgaagttg tgcttaatgc
      601 cgttcaatcc acccctgaaa tgtacgaatg gtttattaat gagtgggtgc atttggtggc
      661 agttaatcct gaaacgcata cactgcatta ctttaagcaa ggtaagtttg tgccttatga
      721 aactttggca acaacactgc cgagcatagg cgattttaat cagctgatgg aagatacacc
      781 gccaatgcgc acaaaccaat tgcctgatgc aaccaaagaa aaccttgcgg tttatctgaa
      841 ttaaaaaggt attttcatgc aatcgttatt acccctttat gtgttcattc cattgattgg
      901 cttctttttg agcctttgct ttgagcgcac acaagaaaaa gccatgtcgc gtattgccat
      961 taccacggtt agtctacatt tgttattggt ggttgttggg ttagtgggtt ggctaagcca
     1021 tggcatgaca acgcaacatt ttaaatactg gcagctgttt gagcatgaag ggtttaagtt
     1081 tgcattggat ttttacctag ataaaatcac catcgtgttt gcgctagtgg gcagcattat
     1141 taccttgctc acgttaatct tcagcaaatt ttacatgcat cgggacgaag ggtttaaacg
     1201 ctttttttgt actttgctgc tgttttatgc gggatttaat tttgctgtat tttctggcaa
     1261 ttttgaaacc ttgtttattg ggtgggagat gcttggtttt acatcttttt tactgattgg
     1321 ctattaccgc aatcgttacc ttcctgttaa gaatgcgttt aaagtgctat cactgtatcg
     1381 tctgggcgat gtttgcctaa tactcaccat gtggatggcg caccatgttt ttcataccaa
     1441 tattactttt attgagttat ccagcaataa ctggcaagag gcctttcata ttgccgaaaa
     1501 acccggaagt ttactgttta tgctgtctat ggtgctggta gcggttgccg ttaaatcagc
     1561 gcagtttcca ttttctagtt ggttgccacg cgcaatggaa gggccaacaa catctagcgc
     1621 cattttttac ggcgccttat cttctcatct aggtatcttt atgcttctgc gtacatatcc
     1681 actgtggcag gctgttccct attttaatta cgcgattatt gtgttgggtc tcatcactac
     1741 ggtcaccgcg atgctgacag tgagagtgca atctaccatc aaaacgcaaa ttgcttacgg
     1801 tactgttacg caattgggta ttatgctcat tgaaattggt ctgggatggc acaccttagc
     1861 cttagtgcat tttgccgggc atgcattttt acgtgcttat caattgcttg tgtcgccatc
     1921 ttcattggga tatatgattc ataatcagtt ctttgagtat caagctaagc caatcaatca
     1981 catgcacgta acaagccgtt tacgcaatac tttttatatg ttgagtgtta aagagtggaa
     2041 cttggatgcg ttacaacaaa attacctatg gcgcccattt aagtggctag gcaagaagtt
     2101 tgtttggcta gaatcgctgg ctagccaagt ggctttaacg ctttttattg tgttgggcct
     2161 gatgacggag cttaactatg taagcttgcc gcaagcagtc agagcatatt ttccagagct
     2221 gtatgctgcg ctttcactta tgtgtatttt ggcggcattt tccagccgtg ggaaccctat
     2281 gcgcgcatgg tggaaaatca tcatgagtca attttttatt gtgttgggcg tgtctctcaa
     2341 tgtgcatgtt gaagctgcgc atgtgtggat ttatctcact actaccatat ctgcagcatt
     2401 agtaggtatg tattgtttac acaacaccta tcatctagat aaagatatta atttaaatca
     2461 atatcatggc tatgtgcaag agcggcctga tttagcactg atctttttaa tcagctgcat
     2521 ggcgctggta gggtttccat ttaccccggc ctttatcgcg attgatttaa tgttcacgca
     2581 aattcatgca catcagtatg tgttggtgtc gcttacaggg tttggtttta ttttcttaga
     2641 attggccgca ataagaattt atgcaaaagt gtttttaggt atccataaga aacatacaca
     2701 tcctacagcc tatcgttctt cttaaataac cgtcgtgtta agcgtccaat ttgagctagc
     2761 agatgaaact tcttgctaag tgattaaacg cctaaagtgg cgttaaagtt aacttaaaaa
     2821 gtgcattaag gtaaccacta aatgctacta atttttctgc ttaacgtggc cttcttttgc
     2881 catgttaatt gagtattttg gaatttcaac gaccaagtca tccttaccca caatcgcctg
     2941 gcaagataaa cgagaattgg gttctaaacc ccaggctttg tctagcaagt cttcttcgtt
     3001 gtcttgggca gcgtttaaag tattgtagcc ttcacgcaca atcacgtggc atgtggtaca
     3061 ggcacagact ttgtcgcagg cgtggtcaat atcaatgtcg tgattgagca ggttgtcgca
     3121 aattgaaacg cccggttcag cctcaaaagc tgtgccttcg gggcagagtt caatgtgcgg
     3181 taatacaata atctgtggca tgtttggttc ctttggtagg cgtgattttt aaattgcgcc
     3241 tacaagttga tgtcttctaa attcttaccc gatagcgcat gggtcacgct cgcattcatg
     3301 cgggcctgcg caaaggcttc cgtggcatgg ttaagtgcct ctacgccttt attgatggca
     3361 actgcgtctt cagcgctagt catgctttgc aagcgcgcaa tttcagcttc aatggcttgt
     3421 ttatcttcat cgttaattaa atggctgtct gccgctagcg ccaaggtcac agcattgata
     3481 atcgcacccg cattgacgcg cgcttcagcc agcatacggg cttttttgtc cgcctcagca
     3541 ctgccgaatg aagatgttaa catacgggtg atttcatctt cagttaggcc gtatgaaggt
     3601 tttaccacca catgcgcttc tacgccgctg gtttgttcgc gtgcagtaac acttaataag
     3661 ccatcggcat ctacctgaaa agtcacgcga atccgcgctg cgcccgctgc catgggcgga
     3721 atgccgcgca actcaaagcg tgcgagggag cggcaatctg ccaccagctc acgttcacct
     3781 tgcaccactt ggatgctcat ggccgtttgg ccatccttat acgtagtaaa gtcttgcgcg
     3841 cgcgctactg gcaacgtgct gttgcgcggg atgatttttt cgactaaacc acccatggtt
     3901 tctaggccta gagagagcgg tgtaacatcc agtaataata aatcatgttc actgcgatta
     3961 cccaccagcg cattggcctg tatggcagcc ccgagcgcta ccactttatc ggggtctaaa
     4021 ttcgtgagcg gggattgctt gaaatagtct tccactgcat gccgtacttg cggcatgcgt
     4081 gttgcgccgc ctaccatgac aacaccttta atatcagcaa tatccaatcc ggcatcgcgc
     4141 attgctttac gtgtgggggt cagcgttttg gtgactaaat gctgcgtgag ttccacaaat
     4201 tgtgtcgcgc tcaatgtaac atccaccaat atgccattgc tgagtttaca cacaatttgt
     4261 gcttcaaagt tatccgtcag ccactctttt gcttggcggg ctttggtcag taaaaggcgt
     4321 gtgtcttctt cgcctagggg tttaaactct tgtggtggcg aaaagttgtt actggtttca
     4381 cgcaccttat cgagtatcca acagtaaata cggtggtcaa aatcgtcgcc accaagcgcc
     4441 gagtcgccat tggtggcaag cacttcaaat acacctttgg tgagctttaa aatactcaca
     4501 tcaaaggtgc cgccacctaa atcgtaaatc acaaacgtgc cttctgcatt gttatctagc
     4561 ccgtaagcaa ccgctgcggc agtgggttca ttcagtaagc gcaatacgtt taacccagcc
     4621 aaacgcgcgg catccttggt ggcttggcgt tgcgcatcgt caaaataagc aggcacggta
     4681 attaccgcac cggtgagctc accgccaagt gagtgctcgg cgcgaacttt gagtgccttt
     4741 agaatttcag ccgaaatctc tacgggcgtt tttaaaccag cgcgcgtgtg taattgcact
     4801 tgcttgttag aagcagcatc ttctacaaat cgatagggaa tatgcgctct atcagcaata
     4861 tcgtgcaaac tgcgccccat aaaacgcttt gccgaaacaa tggtgttcat tgggtcagca
     4921 ctttgctgag cctgcgcctc atggcccacc acaatttcac cattaggtaa atagcgcact
     4981 acagaaggca ataatgcatg gccatactca tcttgtagca cggtgctcat tccgctacgc
     5041 acggttgcta ccagtgaatt tgtggtgcct aaatcaatgc ccacggccaa tttatgctgg
     5101 tgcggtgcgg ctgattggcc tggttcggag atttgtaaca atgccatgtt gtgtatgcgt
     5161 ggttcgtgaa ctatgtttaa acaaataatt gcattgttta gttagtggtt gttaatcatt
     5221 taacacttta atcgagcaat tcaaccgcag tgcggatgtc gctcgataat ttatcaataa
     5281 atatcagttt gcgcgccgtg tcggtagcgc tagtgtaatc tttttttagg tcatactggt
     5341 cagaaaaatc agcttctaaa cgattagatt cagtttttag tgtgcggatt aacgcttcta
     5401 atgcttcaat atttttctct tgtttggcgt cttctaattg ctctcgccat tccatttgtt
     5461 gcattaaaaa atctattggc atggcggtat tggtttcaga aatagcctga atgccttgca
     5521 gttcgagtaa gtatttagcg cgtgctgcgg gctttttaag cgttagatag gcttcgttgg
     5581 cttgtgttgc cacttgcatt gattggagtc tgtcgcttgc tgtagcagtc acaaaacggt
     5641 caggatgatg tgctgattga atttttcggt agctattctc aagtgcctga agatcaagct
     5701 caaagcgtgc atccagacca aaaaaagcgt agtaacttac actcaaactg taaaactctc
     5761 gccgcaacca cactcgtctt tcacgttcgg gttgttgaat ttaaagcctt cattaaggcc
     5821 ttctttagta aagtctaatt ctgtaccatc aatatagacc aagctttttg ggtcaacaat
     5881 gactttcaca ccatggctct caaacgccat atcttcaggg tgcatttcat ccacgaattc
     5941 caaggtgtat gccatgccag agcagccggt ggtttttacg cccaaacgta gcccaatgcc
     6001 cttgccacga ttactcaaga atttttcaac gcggctagcc gcggtttgtg tgagtgtgat
     6061 tgccattaaa atatctttcc ttttttgtca taccgacgaa aatcggtatc cagcgtctct
     6121 gagttagcta agtataaatc ccagtttttt aataagtcac tagattccgt gtcaagcacg
     6181 gaatgacagc ttttagttat gcttctttcg ctgcttgctt cgctttaata tcggctactg
     6241 ccgcttttat ggcatcttcc gccaatactg agcagtgaat tttaacgggc ggcaaggcca
     6301 attcttcagc aatttctgaa tttttaatgg cataagcttc atcaatggtg cggcctttaa
     6361 gccattctgt caccaaagag cttgaagcaa tggctgagcc gcaaccatag gttttgaatt
     6421 tcgcatcttc tataatgccg tcatcattca ctttaatcat taacttcatc acgtcaccac
     6481 aagcgggcgc gccaaccatg ccagtgccca cttgcggatc gtttttatct aagctaccca
     6541 cgttacgcgg gttttcgtag tggtctaaaa ccttatctga atatgccatt ttagttctcc
     6601 t
//
LOCUS       DAOQSI010000120         6594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_11557_length_6594_cov_4.996330, whole genome shotgun sequence.
ACCESSION   DAOQSI010000120 DAOQSI010000000
VERSION     DAOQSI010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 6594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6594
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11557_length_6594_cov_4.996330"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /gene="nhaA"
                     /locus_tag="PKL42_07870"
     CDS             <1..1013
                     /gene="nhaA"
                     /locus_tag="PKL42_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HNU67030.1"
                     /translation="PIVAAIGGMVVPALIYIAFNHEGAAMHGWGIPMATDIAFAVGAL
                     ALLANRVPKALITFLVALAIADDLGAVLVIALFYTNELSLFWLAVSAAFFAVLLTFNF
                     AGIRNAIPYFVIAVILWYALLQSGVHATLAGVLGAFSVPARSKYDPNLFADRINELID
                     RYIASRKKDDSPLTNKKLYTVVQNLEESVNGVQTPLQRLEHIWHLPVAFIVIPIFAIF
                     NAGIPIQFNSLSDTLTHPVMLGVAFGLFFGKFIGITGACWLALKFGIGQLPTGTRFSQ
                     IAAVSILGGIGFTMSIFIAELGFMGQPENLLMAKTGVLLASLLAGVIGYIWLWFLGKK
                     SV"
     gene            1078..1626
                     /locus_tag="PKL42_07875"
     CDS             1078..1626
                     /locus_tag="PKL42_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HNU67031.1"
                     /translation="MNATQPTSRYSTLSIALHWLMLLLFIAVYACIELRELYPKGSDP
                     REALKTWHFMLGLSVFVLVWLRLFARVTSATPSIVPNPPGWQMVLAKLVHLALYALMI
                     VMPLLGWALLSAAGKPIPFFGLELPAMIAENKETGKWLKEIHQTIGTVGYYLIGIHAV
                     AALFHHYVVKDNTLVRMLPNRK"
     gene            1870..2475
                     /locus_tag="PKL42_07880"
     CDS             1870..2475
                     /locus_tag="PKL42_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNU67032.1"
                     /translation="MMLAPKVDMNIFETILARRSVRQYKARVVDQTILRTLLEAAVRA
                     PTAMHQEPWGFILVQDKKLLLQLSAIARPIFLEQIKQQALHNNQKLEDFNQTDFNIFY
                     NANTLILICGKAAVPFVEADCWLAAENLMLAACAMGLGTCIIGSAMPAFSIAKVRSML
                     AIPADYTVVAPIAVGYPDDEITQSKRKSPLILANISEVTGP"
     gene            2529..2993
                     /locus_tag="PKL42_07885"
     CDS             2529..2993
                     /locus_tag="PKL42_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNU67033.1"
                     /translation="MYEHILCPVDGSATSNCGMIEAIKLAKNQQATLRFINVIDLYFP
                     IADATGDFNVVYIDDILRKNGHKVLKGAETEAANVGVKTETKMVETFGHRVSKLILDE
                     ANAWPADLIVMGTHGLRGVERLVLGSDAETVIRKSSVPVLLVKHMHAAESDK"
     gene            3024..5429
                     /locus_tag="PKL42_07890"
     CDS             3024..5429
                     /locus_tag="PKL42_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HNU67034.1"
                     /translation="MSRSKSSIEHAEQQLLSAHELQQINAYWRACNYLAAGMIYLRDN
                     PLLKLPLKPEHIKQRLLGHWGSSPGLSFAYIHINRLIKKYGLNAIFLAGPGHGAPGVL
                     APIYLEGTYSEVYPNKGEDEEGLRQFFKEFSFPGGIGSHCTPEMPGSIHEGGELGYSI
                     SHAFGAAFDNPDLLVTVVVGDGESETGPLATSWHSNKFLNPIHDGAVLPILHLNGYKI
                     NNPTILSRIPHAELENLFKGYGWEPYFVEGDDPLTMHKIMASTLETCVNEIRRIQKNA
                     RENNSATRPSWPMIVLRSPKGWTGPKEVDGHKVEGFWRAHQVPLGKVKEDSKHLAQLE
                     NWLRSYQPEELFDDNGRLIPSLKALAPSGNQRMSANPQANGGLIRKALRMPDFKDHAL
                     ALDAHGKISAPNTKPLGKFLRDIMQRNSQNFRVFGPDENSSNKLDDVYEVSKKTWVNG
                     YLPEDADGGELSPDGRVMEILSEHTVEGWLEGYLLTGRHGFFSTYESFVHVIDSMFNQ
                     HAKWLAMSKAVAWRAPVSSLNLLITSTVWRQDHNGFTHQDPGFLDIVVNKSPEITRIY
                     LPPDVNCLLTVANHCLNSTDYINVIVCDKQKHLQYLDMDAAIQHCTKGLGIWEWASND
                     AGLEPDLIMACAGDIPTKEALAATALLREYFPELKIRFINVVDLYKLTPQNEHPHGLS
                     DRDFDSLFTTDKPIIFNFHGYPYLIHRLTYRRTNHDNLHVRGYKEKGSINTPLELAIQ
                     NEIDRFSLAIAAINRIPALQTIGAHVQEKLRNRQIECCSYAYEYGVDLPEEDAWTWPN
                     LPY"
     gene            5408..6517
                     /locus_tag="PKL42_07895"
     CDS             5408..6517
                     /locus_tag="PKL42_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNU67035.1"
                     /translation="MAELTILTINSGSSSLKASLFQAGSERKNFHYKHLDSNHVKNYA
                     NAFKALLEDLANIQPDIIGHRFVHGGEVADAARLIDEAERARLENLTPLAPLHLASNL
                     LGVDLCAKQFTCPQIACFDTSFHSTLPEIAYRLPIPQSLKIRRFGFHGINYAHVARQL
                     SLLLGETANGRIVIAHLGHGASLCLLENLVSVDTSMGYSTAGGIPMGTRSGEIDPGVM
                     LELSKQFDAAELSKLVYHQMGLIALSDGESSDMQTLIESESESAKFAVAYFARQVRAA
                     IGGYAAKVGGIDALVFTGGIGEHAALVRALICEPLAFLGFHLNADANALNLTLLNTLT
                     SKPILIIPADEEAEIEHLARRWYLTNPITIKGETS"
BASE COUNT         1790 a         1510 c         1523 g         1771 t
ORIGIN      
        1 tacctattgt cgcagccatt ggcggcatgg tggtgcctgc acttatttac atagccttta
       61 atcatgaagg tgcggctatg catggctggg gtatacccat ggcaaccgac attgcctttg
      121 cggtaggtgc gttggccttg cttgctaacc gcgtgccaaa agcgttgatt acttttttag
      181 tggccctcgc cattgcagat gatttagggg cagtgttagt cattgcgctc ttttacacca
      241 acgaattgtc actattttgg ctggcagtat ccgctgcttt tttcgcggtg ctgttaacct
      301 ttaactttgc aggaatccgt aatgcgatac cttattttgt gattgcggtc attctttggt
      361 acgccttgct tcaatctggc gtacacgcaa cgcttgcggg ggtgctaggt gcattcagcg
      421 tgccagcaag atcaaaatat gaccccaact tattcgcgga ccgcattaat gaattgattg
      481 accgctacat tgctagcaga aaaaaagatg atagcccttt aacaaataaa aaactttata
      541 cggtggtaca aaacttagaa gaaagtgtca atggcgtaca aactccctta caacgtttag
      601 aacatatttg gcacctacca gtggcgttta tcgtgattcc gatttttgct atttttaacg
      661 cgggcatacc aattcaattc aattcgctaa gtgacacgct gacacatcca gtgatgctcg
      721 gtgtggcgtt tggtttgttt tttggtaaat ttattggcat tacaggtgcg tgttggctgg
      781 cactaaaatt tggcattggc cagctaccca caggcacacg ttttagccaa attgcggctg
      841 tttctattct gggtggcatt ggttttacca tgtcaatttt tatcgcagaa cttggtttta
      901 tggggcagcc agaaaacttg ctcatggcca aaactggtgt tttgttagct tcactactcg
      961 ccggagttat tggatatatc tggctgtggt ttttaggcaa aaagtccgtt taagcgtaat
     1021 atctaaatgc tacaagcaga aaaataatta tttcaatcaa tgcctaggga ggcaatcatg
     1081 aatgcaaccc aacccaccag ccgttacagc acactgtcta tcgcactaca ttggttgatg
     1141 ctactgctgt tcattgctgt ctacgcctgc attgaattgc gcgaactgta ccccaaaggc
     1201 agtgacccgc gcgaggcact taaaacttgg cactttatgc tgggcttatc cgtgtttgtg
     1261 ctggtttggc tgcgcttgtt tgccagagtt accagcgcca cgcccagcat tgtgcctaat
     1321 ccgccaggct ggcaaatggt gctggcaaaa cttgtgcatt tggcgcttta tgcgttgatg
     1381 attgtaatgc cgttattagg ctgggcattg ttaagcgctg ccggaaaacc aattccgttt
     1441 tttggtttag agttacccgc catgattgcc gaaaacaaag aaaccggcaa atggctcaaa
     1501 gagattcacc aaactatcgg caccgttggc tattacctga taggcataca tgccgtggcg
     1561 gccttatttc atcactatgt tgtaaaagac aatacgctcg ttagaatgct gccgaatcgt
     1621 aaataaagcg tttaaataat cgttgcgccg atggttcagt tgaaactaaa accttaggca
     1681 caacgaatat agccccatca ttttggctac aaactactca gccaaatacc cctctcaaag
     1741 ctaacgcacc tcattttctt caaaaaataa tcccccgcaa actatacgtg ttcgcgcgga
     1801 tttcttaggg cgcatattct tgctactgtg gttgcactgt tactggacct acattgaaaa
     1861 ggtcaatcga tgatgctagc ccctaaggtt gatatgaata tttttgaaac gatacttgca
     1921 aggcgctctg tcaggcaata caaagcgcgc gtggtcgatc aaaccatcct ccgcaccctg
     1981 cttgaagcag cagtgagggc gccaaccgcc atgcatcaag agccgtgggg ctttattctt
     2041 gtgcaagata aaaaattgct cttgcaactt tccgcaatcg ctaggccaat ttttttggag
     2101 caaataaaac aacaagcatt gcacaacaac cagaaattag aagacttcaa ccaaacagat
     2161 ttcaacattt tttataacgc caacacactt attcttattt gcggtaaagc tgccgttcct
     2221 tttgttgagg ctgattgttg gctggccgcc gaaaacctaa tgttagcagc ctgcgcgatg
     2281 ggccttggca catgtattat tggcagcgcc atgcctgcct ttagcatcgc taaagtgcgg
     2341 tcaatgttag ccattccggc cgactacacc gttgtagccc ctattgctgt aggctatccc
     2401 gatgatgaaa ttacccagtc aaaacgaaaa agcccgctga tacttgccaa tatttcagag
     2461 gtaactggcc cgtaaattaa acatcacggc tcaatagaat aaagttaatt cagtcgaaag
     2521 gactaaaaat gtacgaacac atactttgcc cagtagacgg cagcgccact tcaaactgcg
     2581 gcatgataga ggcgataaaa ctggctaaaa accagcaagc aacgttgcgt tttatcaatg
     2641 tgattgacct ttattttcca atcgccgatg ccacaggtga cttcaatgtg gtgtatatcg
     2701 acgatatatt acgcaaaaat ggccacaagg tacttaaagg tgcagagact gaagccgcca
     2761 acgtaggtgt taaaaccgag actaaaatgg tagagacttt tggtcaccgc gtttcaaaac
     2821 tgattttaga tgaagcaaat gcgtggcctg ctgatttaat tgtgatggga acgcacggcc
     2881 ttagaggcgt cgaacgtcta gtgctaggca gtgatgctga aaccgtcata cgcaagagtt
     2941 cagtacctgt cttgctggtt aagcatatgc atgcggctga atcagataaa taatcacttt
     3001 tagcgttttt acaggtggga aaaatgagcc gttcaaaaag cagtatcgag catgcagaac
     3061 aacagctgct atcagcgcat gaacttcaac aaatcaatgc ctactggcgc gcatgtaatt
     3121 acctggcggc tggcatgata tacctgcgcg ataatccact attaaaatta ccgctcaagc
     3181 ctgaacatat taaacagcgc ttgcttgggc attggggttc aagtccaggg ctaagttttg
     3241 cttatattca catcaatcgt ttaattaaaa aatatggtct gaatgctatt tttcttgcag
     3301 gccctggcca cggtgcacct ggtgtactag cacccattta tttagaaggc acctacagcg
     3361 aggtttatcc aaacaaaggc gaagatgaag agggattacg ccagtttttt aaagagtttt
     3421 cattccccgg cggcatcggt agccactgca cgccagaaat gccgggttca attcatgaag
     3481 ggggtgagtt gggttacagc atttcacatg cttttggggc ggctttcgat aatcccgatt
     3541 tattggtgac tgttgttgta ggggatggcg aatcagaaac aggcccgctt gcgacttctt
     3601 ggcactcaaa taaattttta aaccctattc atgacggcgc tgtgttaccc atcctgcacc
     3661 tcaacggtta taaaatcaac aaccccacga ttctctcgcg cattccgcat gcagaacttg
     3721 aaaatctgtt taagggctat ggctgggaac cctattttgt tgagggcgac gaccccctca
     3781 cgatgcacaa aataatggcc agcacgctag aaacatgcgt caatgaaatc aggcgcattc
     3841 aaaaaaatgc gagggaaaat aatagcgcta cgcgtcctag ctggccaatg attgtgttgc
     3901 gcagcccaaa aggctggact ggcccaaaag aagtggatgg tcacaaagtg gaagggtttt
     3961 ggcgcgcgca tcaagtgcct ttaggtaagg tgaaagaaga ttcaaaacac ctagcgcagt
     4021 tagaaaactg gctacgcagc tatcaacccg aagagttatt tgacgataat gggcgactta
     4081 taccttcact caaggcgctc gcgccatccg gtaaccagcg gatgagcgcc aacccacaag
     4141 ccaatggtgg tctcattcgt aaagccttac gcatgcctga ttttaaagac catgcattag
     4201 ccttagacgc tcatgggaaa atttcagccc cgaataccaa gccgctaggc aagttcctgc
     4261 gtgacatcat gcaacgaaac agccagaact ttcgcgtatt cggcccggat gaaaatagct
     4321 ccaataaact agatgacgtt tatgaggtga gtaaaaaaac ttgggtcaat ggctatctgc
     4381 cagaagatgc agatggtgga gagttgtccc ctgatggtcg tgtgatggaa atactttctg
     4441 agcacactgt tgaaggctgg ttagagggct acttgcttac cgggcgacat ggttttttct
     4501 cgacctatga atcttttgta catgtcatcg attcgatgtt caaccagcat gccaaatggt
     4561 tggccatgtc aaaagcggtt gcctggcgtg cgccagtttc atcgcttaac ttgctcatca
     4621 cttctactgt ttggcggcaa gatcacaatg ggttcacgca tcaagaccct ggctttcttg
     4681 atattgttgt gaataaaagc cctgaaatca cgcgtattta tttgccgccc gatgtgaatt
     4741 gcttgcttac ggtggcaaac cattgcctta atagcaccga ctatatcaac gtcattgttt
     4801 gcgacaaaca aaaacacctg caatatttag atatggatgc cgccatccag cattgcacaa
     4861 aaggcttggg catttgggag tgggcgagta acgatgcagg acttgagcca gatttaatca
     4921 tggcgtgcgc tggggacatt ccaaccaaag aggcactggc agccactgcg ctattacgcg
     4981 agtattttcc ggagctgaaa attcgattta tcaatgtggt ggatttgtac aaattaaccc
     5041 cccaaaatga gcacccgcat ggcctttctg accgagattt tgatagttta ttcacaacag
     5101 ataaaccgat tatttttaac ttccatggtt acccgtatct cattcatcgg ttaacgtatc
     5161 gtcgtaccaa tcatgacaac ttgcatgtgc gtggctataa agaaaagggc agcatcaata
     5221 cacctttaga actcgccatt caaaatgaaa ttgaccgttt tagcttagca attgctgcga
     5281 ttaatcgcat ccccgcattg caaacgatag gcgcgcatgt gcaagaaaag ttacgcaata
     5341 gacaaataga atgctgtagt tatgcatatg aatatggggt agatttaccc gaagaagacg
     5401 cttggacatg gccgaactta ccatactaac catcaacagc ggttcatcta gcttgaaagc
     5461 gagcttgttt caggctggta gtgaacgaaa gaactttcat tacaaacatc ttgatagcaa
     5521 tcatgtaaaa aattatgcca acgcttttaa agcattgcta gaagatttgg caaacatcca
     5581 acccgatatt atcgggcatc ggtttgtgca tggtggcgaa gtcgcggacg cggctagact
     5641 gatagatgag gcagagcgcg ctcggcttga aaacctgact ccattagcac ctctgcatct
     5701 tgccagtaat ttactggggg tagatttatg tgccaagcag tttacttgcc cgcaaattgc
     5761 ctgctttgat accagctttc acagcacgct accagaaatt gcgtaccgct taccgattcc
     5821 acagtcactc aaaatacggc gttttggttt tcatggcatc aattatgcgc atgttgcgcg
     5881 gcagctatct ctgctcttgg gcgagactgc aaatggacgt atcgtcattg cgcatttggg
     5941 tcatggcgcc agtttatgcc tactagaaaa tcttgtttct gttgatacca gcatgggcta
     6001 cagcacagcg ggcggcattc cgatggggac gcgcagtggt gaaatcgacc caggggtcat
     6061 gttagaactc agcaaacaat ttgatgccgc ggaattatct aagcttgtgt atcaccaaat
     6121 gggcttaatt gcgctatctg atggtgaaag cagtgatatg cagacgctta ttgaaagtga
     6181 aagcgaaagt gccaagttcg cagtggccta ttttgcacgg caagtacgtg ccgcaattgg
     6241 tggatatgct gccaaggtgg gcggcattga tgcactagta tttaccggtg gcatcggcga
     6301 acacgctgca ctggttcgcg cattgatttg tgagccgtta gcgtttcttg gctttcattt
     6361 aaatgccgat gcaaatgcac tcaacctgac cctcctcaat acccttacat cgaaacctat
     6421 cttaattatt ccagccgatg aagaagcgga aattgaacac ttagctcgac gctggtattt
     6481 aacaaacccg ataacaataa aaggagaaac ctcatgacca taagtgcaat ttgtaatcgt
     6541 gaagtcatta ccgtgcaacg tgatgccacc atactgcatg tggcctcgct catg
//
LOCUS       DAOQSI010000121         6358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_12214_length_6358_cov_3.949865, whole genome shotgun sequence.
ACCESSION   DAOQSI010000121 DAOQSI010000000
VERSION     DAOQSI010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 6358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6358
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12214_length_6358_cov_3.949865"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..868
                     /locus_tag="PKL42_07900"
     CDS             98..868
                     /locus_tag="PKL42_07900"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HNU67036.1"
                     /translation="MQIKILGCSGGIGGDLRTTSMLIDDNILIDAGSGVGDLSMDALL
                     KIDHIFVTHAHLDHIAFIPPLLDTVLGFRTAPVTIHALQATIDTLRAHIFNWKIWPDF
                     NAIPSAEAPFLIYSAITLGETVQLNGRKITPVPANHVVPAVGYHLEGTEASLIFTGDT
                     TTNDALWPLVNKMHNLKYLIIETAFRNDELELAKLSKHLCPSLLVDELNKITLTQPLE
                     VFITHLKPGEGDVIMQEIAQSQIRQPVQALQNQQQFMI"
     gene            923..1993
                     /locus_tag="PKL42_07905"
     CDS             923..1993
                     /locus_tag="PKL42_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNU67037.1"
                     /translation="MPVAQSHLEQFEPMAKLSLGRRIELADLVYAEKVNKDIDPLRMN
                     ITKAAQSIYLLKGDLELTLFNDKKIKLVAGSPAAKYPINSNGKVKETKALTDVAIMRV
                     DTDLLDIMMTWDQISDSKPAPTSTVVVKQIDHERSAADWMKDTQIFSAYNLQKGIFSH
                     LPAVNIEEMFNRMTSIEVTAGQVMIQQGGVGDYYYLIQNGTAVVSRMVDSTQPPVLLA
                     ELKQGRAFGEDALISEAKRNATVTMKTDGRLLRLNKTDFVELLKAPLITQVDMQEAQQ
                     KVAEGAMWIDARLPSEFEDDHIAGAMNIPLNEVRQHASSLDKSKTYIVYCQTGRRSSA
                     AAFVLTQCGINAVVLKGGSRNL"
     gene            complement(2375..2779)
                     /locus_tag="PKL42_07910"
     CDS             complement(2375..2779)
                     /locus_tag="PKL42_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HNU67038.1"
                     /translation="MKQVQKGFTLIELMIVVAIIGILAAIALPAYSDYQAKAKVTAGL
                     AEVAGYKTAFELLINENTTPTTLLLGMPGSTSNCSGFTVTGTTIVCTITNAPSQVQGK
                     TVTLTRAAATGAWSCATNAVNKYAPGKGCPGV"
     gene            complement(3453..3989)
                     /locus_tag="PKL42_07915"
     CDS             complement(3453..3989)
                     /locus_tag="PKL42_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNU67039.1"
                     /translation="MKKILALAALSLSMSLVPFTASAHGPSPQKVEKTVTIKADPAKV
                     WAIVKDFGNIHGWHPGVANTKVEKKGEDTFRTLNFKDGGHVYEKLRSIDDASMKIKYE
                     IIEGTLPLTDYNAVMTVEKGDKAGETKVTWMGRFYRLYKLNPPIPEGQDDATAVKAIE
                     GIFDSGLANLQKVAEGSK"
     gene            complement(4337..5284)
                     /locus_tag="PKL42_07920"
     CDS             complement(4337..5284)
                     /locus_tag="PKL42_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HNU67040.1"
                     /translation="MTASLMLGACNAANDSAQHSSFAYITNQGEHTVSIINTETNQVT
                     STISVGKAPVGVAVSTQLQRAYISNVESQDISVINTQTNTVIDTIKIKGSPVGLALAP
                     DDNTLYVADWFENRVLAINTLDPQKVREIKVGDAPAGLVVSPDSKTLYISNRDSNDIA
                     VIDTKTLTIQKRIAVGKHPFGIALSKSGKTLISVNVMDDSVSVINLHNNTLKTIKVSY
                     HPYCAVLTDDENTLYVTNTQDDSVSVIDMRTQKTIATIGVGSTPEGISLNPANQRAYV
                     ANWGSNNVSVIDTNTNTLIATIKTGEKSRAFGNFILPKQ"
     gene            5412..5897
                     /locus_tag="PKL42_07925"
     CDS             5412..5897
                     /locus_tag="PKL42_07925"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HNU67041.1"
                     /translation="MLDRDGFRPNVGIIICNANNQVFWGKRIREHAWQFPQGGINHGE
                     SPEQAMYRELMEEVGLKPEHVQILGRTKDWLRYEVPTTWVKREYRGSYKGQKQIWYLL
                     RLIGRDTDVSLRATDQPEFDAWRWNDYWVPLEDVIEFKRKVYEMALNELAPHLHSKGG
                     K"
     gene            complement(5970..>6358)
                     /gene="asd"
                     /locus_tag="PKL42_07930"
     CDS             complement(5970..>6358)
                     /gene="asd"
                     /locus_tag="PKL42_07930"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883441.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HNU67042.1"
                     /translation="YVPGDLFSVNPTTAEGVPGLFARNERVVCEFSSAQHGSFVMVLV
                     GATIVGSMATVWHTAKDRIINPPRQPNVHTWDYSDQNIHLKQNDEMGRFLLGSTVVLL
                     FQQDALQFNTDWQPARAVSLGEVMGN"
BASE COUNT         1758 a         1318 c         1417 g         1865 t
ORIGIN      
        1 tgcacgtatt gtccatttta ggcagaatcc gccagccaat gattgggatg gtgtgttcat
       61 tgttgaaaca aaataaaaat taaacaaaaa tttaaccatg caaatcaaaa ttttaggctg
      121 tagcggcggt attggtggcg acttacgcac cacctccatg ctgattgacg acaatatttt
      181 aattgacgcc ggttctggcg tgggtgattt gtcgatggac gccttgctta aaattgacca
      241 catttttgtc acgcatgcgc atttagacca cattgctttt attccccctt tgcttgatac
      301 cgttttgggt tttcgcacag cacctgtcac tatccatgcg cttcaagcaa cgatagacac
      361 cttacgcgcc catattttta actggaaaat ctggccagat ttcaatgcaa ttcccagcgc
      421 cgaagcgccg tttttaattt atagcgcaat cacgctgggt gaaactgttc aattaaatgg
      481 ccgaaaaatc acgccagttc ccgctaatca tgtggtgccg gcggttggtt atcatttaga
      541 aggcactgaa gcgagcttga tttttacagg tgacaccacc accaatgacg cgctttggcc
      601 gctagtgaat aaaatgcaca atttaaaata ccttatcatt gaaaccgcat ttagaaacga
      661 tgagctagaa ctggcaaaac tatccaagca tctttgccca tcattgctgg tggatgagct
      721 gaataaaatc actttaaccc agccgcttga agtgtttatt acccatctaa aacccggtga
      781 aggtgacgtg attatgcaag aaattgcaca aagccaaatt cgccaacccg ttcaggcgct
      841 tcaaaatcaa caacagttta tgatttaaac aatcgcaatt tagttttaat atcaaccatc
      901 aacttacagc agggtcctca acatgccggt agcacaatcc catcttgaac aatttgaacc
      961 gatggcaaaa ctttctctcg gccgcagaat cgagttagcc gatttggttt atgcagaaaa
     1021 ggtgaataaa gacattgacc cattacgcat gaatattacc aaagccgcgc aatcaattta
     1081 cctgcttaag ggtgatcttg aattaacgct ctttaatgat aaaaaaatta agctggtggc
     1141 tggtagccca gcggcaaaat atcccatcaa tagcaatggt aaggtcaaag aaaccaaagc
     1201 gctgactgat gtggccatta tgcgggtaga cacggattta ctcgacatca tgatgacgtg
     1261 ggaccaaatt tcagacagta agccagcgcc aaccagtacg gtagtcgtta aacagattga
     1321 ccacgagcgt agcgccgccg attggatgaa agatacgcag atttttagcg cttacaatct
     1381 gcaaaaaggc atcttcagcc acctgccagc cgttaatatt gaagaaatgt ttaatcggat
     1441 gaccagtatt gaagtcacgg ctgggcaagt gatgatacaa caaggcggtg tgggggatta
     1501 ctactacctg attcaaaacg gcaccgcagt ggttagtcgt atggtggata gcacccagcc
     1561 tcccgtgcta ttggccgaac tcaaacaagg ccgcgctttt ggtgaagatg cgctgatatc
     1621 agaagcaaaa cggaatgcca ccgttaccat gaaaaccgac ggaaggttat taaggctcaa
     1681 taaaacagat tttgttgagc tgttaaaagc gccgctcatc actcaagtgg atatgcaaga
     1741 agcacagcaa aaagtagccg aaggcgccat gtggatagat gcacgtctgc caagcgagtt
     1801 tgaagatgat cacatcgcag gcgcgatgaa cattccactc aatgaagtgc gccaacatgc
     1861 cagcagcctt gataaaagca aaacgtatat tgtgtattgc caaaccggcc gtcgcagctc
     1921 agcggctgca tttgtgttaa cgcaatgtgg catcaatgcg gtggtgttaa aaggtggctc
     1981 aagaaattta tagagacttt tgcacgaatg attgttaaat tcaaaaaacc tacccactcg
     2041 ttattccagc ctgcgctgga gtgacgaaac cgtagatttt gaaggtaaaa tttcgtgtaa
     2101 aggtttctta taacttttcc tgctgcctag attgcaatca agaaaaaatg ccgtgtgcca
     2161 ttatcatgta gccgtgatgt atcgtagcga atttaaggta aaagtctgcg caaaacaata
     2221 ttccccccaa ttatgcagag ggggtagcct cgatgcaacg cattagactc gaaggagcaa
     2281 ccacgacccc aaaaacaatt acccccgcaa tttgcggggg taattgtttg ttacgttaaa
     2341 cctcgattac attacatcgt ggctacgctt gctattaaac gccagggcaa cctttgcctg
     2401 gagcgtattt attaactgcg tttgttgcgc atgaccacgc tcctgtagca gctgcgcgtg
     2461 tcagtgttac tgttttaccc tgaacttgtg aaggtgcgtt agtgattgta cacactatag
     2521 tggtgccagt tactgtaaaa ccgctacaat ttgaagttga acctggcatg cctaaaagta
     2581 aagtcgttgg cgtagtattt tcattgatta gcagctcaaa agctgttttg taaccagcaa
     2641 cttctgccag accagcagtt actttagctt tagcttgata gtctgagtat gcaggcaaag
     2701 caatcgcagc caaaataccg ataattgcca caacaatcat taactcgatt aaggtaaaac
     2761 ctttttgaac ttgtttcatt ttgaaatcct ttataaaaat ttagtaaaag tacactgata
     2821 aataaaacta cgctttaaac aacatttaaa acatattttt gttactttaa gcgacgttac
     2881 cagcgcttga atatgtatta gcaatggctg tgccaacttt tattatttag cgattttgtt
     2941 tggtatataa aatttaactt attgatttaa atgaactaaa tagttatttt atttgtgtat
     3001 ttaatcaata tgactttatt tgaatcttca aaaaggcttt tacagtcttt aataacgata
     3061 gcaggttgac aataattgtc actgtggcac tcaaagtggt cacttcaatt tacggcggtt
     3121 tactgaattt cttgatagct taacactacg cctgttgtaa gcgcgctttg cacaaaacaa
     3181 aagattggtt caatttttaa caacaatatc caaaaaaccg tcattcggcg caggtggtaa
     3241 tgacgaagtc gataggcttt aggccaaaaa tacttcgtgc aaaagtccct tacaaaataa
     3301 aaaacggcaa ccgagattgc cgttttttaa ttttaatacc ttgttttgtt taacttacca
     3361 aaatttccac caaggtttgg ttttggttgt gctgcttact gtgttacttg tggtgttgct
     3421 aggggtcgag tttgtgccag ttgcctggct cattactttg acccttcggc cactttctgc
     3481 aagtttgcta agcctgaatc aaaaatgcct tcaatcgctt taactgcagt agcgtcatct
     3541 tggccttcag gaataggtgg gttcaatttg tataaacggt aaaagcggcc catccatgtg
     3601 acttttgttt caccagcttt atcgcctttt tcaaccgtca tcactgcatt gtagtcagtg
     3661 agcggtaaag tgccttcaat gatttcgtat ttaattttca tgctggcatc atcaatgctg
     3721 cgtagttttt catacacgtg gccgccgtct ttaaagttca gcgttctgaa tgtatcttca
     3781 ccttttttct ccactttggt gttagccacg cctgggtgcc agccgtgaat gttgccaaag
     3841 tcttttacaa tcgcccaaac ttttgctggg tctgctttga tagtaacggt tttttcaact
     3901 ttttgtggcg atgggccgtg cgcagatgcg gtaaacggaa ctagagacat gcttaaacta
     3961 agtgcggcta gcgctaaaat ctttttcatc taactttctc cataactctc gatacgagag
     4021 gaattattaa aaacaacaat gcattataaa tgagaacgct gttttataaa atgccttgtt
     4081 gattgtggtt gtgataacga tttttgtgct tgcttggttg acagataaaa tgggtttaat
     4141 tcaggcttaa aatcccctag aaaaaattta tgctctggaa gcctttattt atagggctcg
     4201 cagagcatcg aaaaaggttg gtatggtttt aggcattaaa aatgcgtttt atatgttact
     4261 tttcaaaaga aatttaacag taatttatag ctgtagaatt tgcgctttta atgatttggc
     4321 aatacaaatt gcgcttttat tgtttgggca atataaaatt cccaaatgcc cgactttttt
     4381 cacctgtttt aatggtggcg attaatgtat tggtgttggt gtctatcaca ctcacattat
     4441 tgctgcccca gttggcaacg taggcgcgct ggttggcggg gtttaggcta atgccttcag
     4501 gcgtgctgcc tacgccaatg gtggcaatgg ttttttgtgt gcgcatatca atcacgctca
     4561 cgctgtcatc ttgtgtgttg gtgacataga gtgtgttttc gtcatctgtg agcaccgcgc
     4621 aataggggtg gtaactcact ttgatggtct ttagcgtgtt gttgtgcagg ttaatcacgc
     4681 tcacgctgtc gtccattaca tttacgctaa tgagcgtttt gccgcttttg ctgagcgcaa
     4741 tgccaaaagg gtgcttgcca acggcaatgc gtttttgaat cgtaagcgtt ttggtgtcaa
     4801 tgactgcaat gtcattgcta tcacgattac taatgtagag ggttttgcta tcggggctta
     4861 ccactaggcc agcgggcgcg tcgcccactt tgatttctcg caccttttga gggtcaagcg
     4921 tattgatagc cagcacgcga ttttcaaacc aatcggccac atataaggtg ttgtcatcag
     4981 gcgctaaggc aaggccaacg ggtgagcctt tgattttgat ggtatcaatt acggtatttg
     5041 tttgtgtgtt aatcaccgaa atatcttggc tttcaacatt tgaaatataa gcccgttgca
     5101 attgagtaga aacagccacg cccacaggcg ccttgcctac tgaaattgtg ctggtgactt
     5161 gattggtttc ggtgttgatg atcgagaccg tatgttcacc ttgattggta atataggcaa
     5221 aactgctatg ttgcgctgaa tcattggcgg cgttgcatgc gcccagcatg aggcttgccg
     5281 tcaagattaa aaatttgcta aaattttttc tgtatcgatt catgaggtta cgatttatat
     5341 gtaaatggtc actttcaata atggccgaaa tgtgaaaaaa ttgcactaaa tattttaggt
     5401 agcataacgc aatgttagat cgagatgggt ttcgcccaaa tgttggcatt attatttgta
     5461 atgccaacaa tcaggtgttt tggggtaagc gtattcgtga gcatgcttgg cagtttccac
     5521 aaggtggtat caaccacggg gaaagcccag agcaggccat gtatcgtgaa ctcatggaag
     5581 aagtaggctt aaagcctgag catgtgcaaa ttttaggccg caccaaagat tggttgcgct
     5641 atgaagtgcc caccacctgg gttaagcgcg agtatcgcgg cagctacaaa ggccaaaagc
     5701 aaatttggta tttgttgcgc cttattgggc gtgatacaga tgtttcgctc cgagcaaccg
     5761 accagccaga gtttgacgca tggcgatgga atgactattg ggtgccactt gaagatgtga
     5821 ttgagtttaa gcgcaaggta tatgaaatgg cgcttaatga gttggcacca catttacata
     5881 gtaagggtgg aaagtaacat ttgtaggagc gatttttaaa tcgcgatggt catatcgcat
     5941 tcgcgattta aaaatcgctc ctacaattat caattgccca ttacctcgcc aaggcttact
     6001 gcacgagctg gttgccaatc tgtattaaat tgcaatgcgt cttgctgaaa aagcaagaca
     6061 accgtcgagc ctagcaagaa tcttcccatt tcatcgtttt gttttaaatg gatattttgg
     6121 tcgctgtaat cccaagtgtg aacgttgggc tggcgcggcg ggttgatgat gcggtcttta
     6181 gccgtgtgcc acacggttgc catactgccc acgatggtgg cgccaaccaa taccatcaca
     6241 aagctgccgt gttgtgcaga agaaaattcg caaactaccc gttcatttct tgcaaataaa
     6301 cctggcacac cttctgcggt ggttggattg actgaaaata agtcgcctgg cacgtagg
//
LOCUS       DAOQSI010000122         6318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_12353_length_6318_cov_4.910746, whole genome shotgun sequence.
ACCESSION   DAOQSI010000122 DAOQSI010000000
VERSION     DAOQSI010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 6318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6318
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12353_length_6318_cov_4.910746"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..283)
                     /locus_tag="PKL42_07935"
     CDS             complement(62..283)
                     /locus_tag="PKL42_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67043.1"
                     /translation="MKKLFYIDYPQERFEGHTHRYRCALCKEETTKINGALEGHLPSC
                     QYRIDLEKAGYEPEPVVAHLELNTSDEVD"
     gene            703..1689
                     /locus_tag="PKL42_07940"
     CDS             703..1689
                     /locus_tag="PKL42_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF533 domain-containing protein"
                     /protein_id="HNU67044.1"
                     /translation="MNHNQQKAILTIALFAAFADGKKDDREREQIRQLAETLGSDTDT
                     QDIAKLYQDVLLKRVSVDSATILLDDQSHRQLAYEMAVCVCDADGIQTATERRFLDDL
                     KVALGLSAEQIELVEHEAGELASLVDNPAFPATVVTLSSQEKASSEPVIAAVANVSEA
                     ELEKMILNYSILNGALELLPQSWASMAIIPMQIKMVYRIGKSHGIELDTGHIKEFITA
                     AGVGLTSQYLEQFGRKLLGGLLGKMAGKTVGKIGSAATGVAFSFATTYALGQVAKRYY
                     AGGRVMNSNILRDTFQNLLGPAKQLQTQYLPQIQQKASTLDVTQVMSMVRGA"
     gene            complement(1783..2481)
                     /locus_tag="PKL42_07945"
     CDS             complement(1783..2481)
                     /locus_tag="PKL42_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrdJb"
                     /protein_id="HNU67045.1"
                     /translation="MTIKIDKKIVSYNLVTEEDKAKALEAKIQEDNAKVIQLGEPLSR
                     PDKLVGNTYKIKTPVTEHALYITINDVVMNEGTAQEHRRPFEIFINSKNMEHFQWIVA
                     LTRVMSAVFRKGGDVTFLVEELKSVFEPSGGYFKKGGKFVPSLVAEIGEVVEQHLQEI
                     GMLKKPGLDEHQQKLVAEKKAEYLEKHAKSGEEANDDGFPKGAQLCKKCNTKAAIIMD
                     GCLTCLNCGESKCG"
     gene            complement(2509..4674)
                     /locus_tag="PKL42_07950"
     CDS             complement(2509..4674)
                     /locus_tag="PKL42_07950"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HNU67046.1"
                     /translation="MLKAVESATQSQAGAEKEIPIQPVSLDIWDKKYRLKTKTGEYVD
                     QNMDDSYSRVARALADVETTEDKRHEWHTKFLWALRHGAIPAGRITSNAGALEHKPAT
                     STINCTVSGVVEDSMDNILNKVHEAGLTLKAGCGIGYEFSTLRPKGAFVAGAGAYTSG
                     PLSFMDIYDKMCFTVSSAGGRRGAQMATFDISHPDVTDFIKAKRENGRLRQFNLSCLI
                     TKEFMEAVKADAEWKLAFPVTEKEAIIDGLNTNDVAQVVWREWPVKGKYLTRLDGVDA
                     GKVACRVYKTIKARRLWDVIMSSTYDFAEPGFILIDRVNEMNNNWFCENIRATNPCGE
                     QPLPPYGACLLGSVNLTMFVKKPFTDEAYFDWKEYREVVSIFTRMLDNVVEINGLPLQ
                     QQRDEIMRKRRHGMGYLGLGSALTMMKMKYGEEESIKFTEEVTRVMAEEGWNVGVQLA
                     EEKGPAPIMEEKFEVTADMLAKRPEMAADGIKVGQKIAGKVLLGKYSRYMQQFPEGLR
                     NQIATKGVRFTHHSSIAPTGTISLSLANNASNGIEPSFAHHYARNVIREGKKSKEKVD
                     VFSYELLAYRELINPKAMPFSDKADEKLPDYFLDSSTIMAKAHVDIQAASQKWIDSSI
                     SKTINVPTDYDFEDFKNIYLYAYDKGLKGCTTFRFNPEAFQGVLVTEKDLENTTYKFT
                     LDDGTEIEAKGNDEIEYDGEIHSAANLYDALKEGYYGKF"
     gene            5049..6311
                     /gene="waaA"
                     /locus_tag="PKL42_07955"
     CDS             5049..6311
                     /gene="waaA"
                     /locus_tag="PKL42_07955"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HNU67047.1"
                     /translation="MNRFFYSILLYLVLPFVPLKLLLRGIKQPDYRQHWRERFGFYQS
                     IRSKPVIWLHCVSVGETRAAAPLVHALLNQYPHHQILLTHTTPTGRATSEQLFGDKVQ
                     RVYLPYDLPFAVKRFLRYFNPVVGVLMETELWFNLIAGCKQQNIPLLLVNARLSEKSA
                     QGYSKLGHLVNTGLQSLSAIAAQTEQDAARLTALGANNVQVAGNLKFDVIPPADVQQQ
                     GWQMRHLWGENRPVFLAASTREGEEAIIIEAITAAKMPNLLTVIVPRHPQRFNEVEAL
                     MQQCGLRYQKRSTLKQTADDETQWVLGDSMGELFTYYASADICLVGGSLLPFGGQNLI
                     EAMRMGKPVLIGEHTYNFKEVSALAVAQCAAWRVADAQSLTKAIQSLVATPHQQAEMG
                     AKGLALCQASQGATARTLKLIQEYIRVA"
BASE COUNT         1605 a         1378 c         1436 g         1899 t
ORIGIN      
        1 tataaggctc acagggcatc gaaaaaggtt gtgggtaaaa tgcgctgttt tgcttgtgtg
       61 gttaatcgac ttcatctgaa gtattgagtt ctaaatgcgc caccaccggc tctggctcat
      121 aacccgcttt ttctaaatca atgcggtact gacagctagg caaatgcccc tcaagcgcac
      181 cgttgatttt ggtggtttct tccttacaca acgcgcaacg gtaacggtgc gtatgcccct
      241 caaaacgctc ttgcgggtaa tcaatataaa atagtttttt cataacaggc tcccgaggtg
      301 gtttaagcca tcgtacacct agcggattgg ggaggcaata agggtttagc gtggaggtga
      361 atgtagcagt tgtattcggc gcattacact tcgttaatgc gcccttgctg atggcgaaaa
      421 ataagaatat tgcgcctgta cggaacatga cagttttttt attgtcaatt ttatctagaa
      481 taaaaaattg ttgctggcgt tagctggtgt gcttgctttg cgtgcaattg ctgtacattg
      541 ctagcctgta taggtcactt ttggcgcaat agatttacgg cggaaggctg gctgtgagct
      601 acattggtag cttcaagcct attgttccta gatgagatgc gtaagtttgc cattaaatta
      661 tttcacttga ttactaaaag cgtaaaggat aaaacttcat atatgaacca caatcaacaa
      721 aaagccattc taaccattgc tttatttgcg gcctttgcag atggcaaaaa agatgaccgt
      781 gaacgtgagc aaattcgtca gttagctgaa acactaggta gcgacacaga cacccaagat
      841 attgccaagc tatatcagga cgtattgctc aaacgtgtaa gtgtggatag cgcgaccata
      901 ttacttgatg accagagcca tcgccaactt gcctatgaaa tggccgtttg cgtgtgtgat
      961 gcagatggta tacaaacagc gactgaacga cgatttttag atgacctgaa agtagcgctt
     1021 gggctaagtg cagagcaaat agaactagtg gagcatgagg caggtgagct tgccagtttg
     1081 gttgataatc cggcgtttcc cgcgacagtt gttacgctgt catcccaaga aaaagcctca
     1141 agtgagcctg ttattgccgc cgttgcaaat gtttcagaag ctgaacttga gaaaatgatt
     1201 ctaaattatt caattctgaa tggtgctttg gaattattgc cccagtcctg ggcatccatg
     1261 gcgattattc ccatgcagat taaaatggtg tatcgcatag gcaaatcgca cggcatcgag
     1321 ctcgacacgg gccacattaa agaatttatt accgcagctg gcgttggtct cacttcacag
     1381 tatcttgagc agttcggtcg taaactattg ggtggactat tagggaaaat ggcgggtaaa
     1441 actgtgggta agattggcag cgccgcaacc ggcgtggcat tctcctttgc aaccacctat
     1501 gcgcttggac aagttgccaa acgctactat gcaggtgggc gtgtgatgaa tagcaacatt
     1561 ttacgcgaca catttcaaaa cctacttggc cctgcaaagc aattgcaaac acaataccta
     1621 ccacaaattc agcagaaagc tagcacgctt gatgtgacac aggtcatgtc tatggtgcgt
     1681 ggcgcgtaaa ataggcaagt gaaaaagttg ttgtggcggt ataggatgag tgttgattat
     1741 ctatgccgct acaaccgccc tgtgtaaaat tatttagcct ccttacccgc acttactctc
     1801 accacaattc aagcaagtca aacaaccatc cataataatc gcggctttgg tgttgcattt
     1861 tttgcatagt tgcgcgcctt ttggaaagcc atcgtcgttg gcttcttcac ctgattttgc
     1921 gtgtttttct aggtattcag cttttttttc agccaccaat ttttgctggt gttcatctaa
     1981 gcccggtttt ttgagcatgc cgatttcttg caggtgttgc tctaccactt cgccaatttc
     2041 tgccactaaa ctcggcacaa atttgccgcc ttttttaaag tagccgccac ttggttcaaa
     2101 cacgcttttg agttcttcca ccaaaaaggt cacgtcgccg cctttgcgaa acaccgctga
     2161 catcacgcgg gtgagcgcaa caatccactg aaagtgctcc atgttttttg agttgatgaa
     2221 aatctcaaac gggcggcggt gctcttgggc ggtgccttcg ttcatcacca cgtcgttaat
     2281 ggtgatgtag agcgcgtgtt cagtgactgg cgttttgatt ttgtaagtgt tgcccaccag
     2341 tttatctggg cggctgagcg gctcgcctag ctgaatcacc ttggcgttgt cttcttgtat
     2401 tttggcttca agcgctttgg ctttgtcttc ttcagtgact aggttgtaac ttacaatttt
     2461 tttgtcaatt ttaatggtca tgttgttttc tccagatacg gctgttcatt agaatttacc
     2521 gtagtagcct tcttttaagg catcgtatag gtttgcggct gagtgaattt cgccatcgta
     2581 ttcaatttca tcgttacctt tggcttcaat ttcagtgccg tcatccaacg taaatttgta
     2641 ggtggtgttt tctaagtctt tttctgttac caacacgcct tggaatgctt cagggttaaa
     2701 tctgaacgtg gtgcaacctt tcaggccttt atcgtaagcg tagagataga tgtttttaaa
     2761 gtcttcaaag tcgtaatcag tgggtacgtt aatggttttt gaaattgagc tatcaatcca
     2821 tttttgtgac gcggcttgaa tatccacatg cgctttggcc ataatggtgg atgaatctaa
     2881 gaaataatca ggtagtttct catctgcttt atctgaaaac ggcatggctt ttgggttaat
     2941 caattcacgg taagccagca gttcataaga aaatacatcg actttttctt tagatttttt
     3001 gccttcacga atcacgttac gtgcatagtg gtgcgcgaaa gatggctcaa tgccgttgct
     3061 ggcgttattg gccaagctta atgaaatcgt gcctgttggc gcaattgagc tatggtgcgt
     3121 aaagcgtacg cctttggtgg caatttggtt acgcaaacct tcagggaact gctgcatata
     3181 gcggctgtat ttgccgagca atactttacc tgcaattttt tggcccactt taatgccatc
     3241 ggccgccatt tctgggcgtt ttgccaacat gtcagcagtc acttcaaatt tttcttccat
     3301 aatcggtgct gggccttttt cttcagccag ttgcacacca acattccaac cttcttctgc
     3361 cattacgcgg gtgacttctt cagtgaattt aatggattct tcttcaccgt atttcatttt
     3421 catcatggtg agggctgagc ctaagcctaa atagcccatg ccgtggcggc gtttacgcat
     3481 aatttcgtca cgttgttgtt gcaatggcaa gccgttgatt tcaaccacgt tatcaagcat
     3541 gcgggtgaaa atgctaacga cttcgcggta ttctttccag tcaaaatacg cttcgtcagt
     3601 gaatggtttt ttcacgaaca tggttaaatt aactgaacct aacaaacaag cgccataggg
     3661 tggcaatggt tgctcgccac aagggttggt agcgcggatg ttttcgcaga accagttgtt
     3721 gttcatttca ttaacgcggt caatcaaaat aaaacctggc tcagcaaagt cgtacgtgct
     3781 gctcataatc acgtcccata aacggcgcgc tttaatggtt ttgtacacgc ggcaagccac
     3841 tttgccagca tctacgccat ctaatctggt gaggtatttg ccttttaccg gccactcgcg
     3901 ccaaaccact tgggcaacat cattggtatt taagccatca ataatggctt ctttttctgt
     3961 cacggggaac gccagtttcc attctgcatc ggcttttacc gcttccataa actctttggt
     4021 aatcaaacag cttaaattga attggcgcaa acggccgttt tcacgtttag ctttaataaa
     4081 gtctgtgacg tctgggtggc taatatcaaa cgttgccatt tgtgcgccac ggcggccgcc
     4141 cgctgatgac actgtaaaac acattttgtc gtaaatatcc ataaaagata gtgggccact
     4201 ggtataagca cccgcacccg caacaaacgc gccttttgga cgtagcgtag agaactcata
     4261 gccaatacca cagcccgctt taagcgttag gcctgcttca tgcactttat ttaaaatatt
     4321 gtccatgctg tcttcaacca cgccagatac tgtgcaattg atggtagacg tggctggttt
     4381 gtgctctaac gcacctgcgt tagatgtaat gcgcccagcg ggaattgcgc catgacgcaa
     4441 tgcccataaa aatttggtat gccattcatg gcgtttgtct tcagtggttt ctacatcggc
     4501 taaagcgcgt gctacacggc tgtacgagtc atccatgttt tgatcaacat attcaccagt
     4561 ttttgttttt aggcgatatt ttttatccca aatgtctaac gatacgggct gaattggaat
     4621 ttctttttca gcgcccgctt gtgattgggt tgctgattca actgctttta gcatggatac
     4681 ctctcctgga ctttaagctt agcgcgctaa ctttttgtta attcttcgct aagcccgtga
     4741 ttttatttaa taaactatta ttttagtgaa aaatagggtg cttaaacaca acatgttgtg
     4801 tcttgagcag caatttaagc ctttattttg tgtacgtcaa ctataatttt catgcatttt
     4861 ttgcagtaat ttttgtgatt ttgcagtaat ttaaagcctg tatttgcatt gaagtaaggt
     4921 gagtaatcac ttacatcaaa cacaattatt taatttttac aaaaataaat tctacgcaaa
     4981 agttgctgat tttcgactta cttgatgcag ttaaaaacaa tcgttaccgt tttatgagaa
     5041 aatgccttat gaatcgcttt ttctattcca tcttgcttta tttggtgttg ccgtttgtgc
     5101 cgcttaaatt acttttgagg ggcattaagc agccagatta tcgccagcat tggcgcgagc
     5161 gttttggctt ttatcaatct atacgcagca aacctgttat ttggttgcat tgtgtgtcgg
     5221 taggtgaaac ccgcgcagcg gcacctttgg tgcatgcgct actgaatcaa tatccacacc
     5281 atcaaatttt attgacccac accacgccca ctggccgcgc caccagcgag caattatttg
     5341 gcgataaagt acaaagagta tatttacctt atgacctgcc ctttgctgta aagcgctttt
     5401 taagatactt taaccccgtg gttggggtgt taatggaaac tgaactttgg tttaatttaa
     5461 ttgcgggttg taagcagcaa aatattccct tgttattggt caatgcgagg ctctcagaaa
     5521 aatccgcaca aggttactca aaactaggcc atttagtgaa tactggattg caaagcctgt
     5581 cagcaattgc agcacaaact gagcaagatg cagcgcgcct aactgcgctt ggcgcaaata
     5641 atgtgcaggt agctggcaat ttaaaatttg atgttatacc gcccgcagat gtgcagcaac
     5701 aaggctggca gatgcgacat ttatggggtg aaaatcgccc ggtattttta gccgccagca
     5761 cacgtgaggg tgaagaggcc atcattatag aagccattac agccgctaaa atgccaaatt
     5821 tgctgacagt gattgtaccg cgccacccac aacggtttaa tgaagttgaa gccttaatgc
     5881 agcagtgtgg attacgttat caaaaacgct ccacacttaa acaaacggcg gatgatgaaa
     5941 cgcaatgggt gcttggcgac agcatgggcg aattatttac ctactatgca agtgcagata
     6001 tttgcttggt aggtggcagc ctgttgccct ttggcgggca aaacttaatt gaagcgatgc
     6061 gaatgggtaa gccggtattg attggtgagc atacctataa ctttaaagaa gtgtcagcgc
     6121 ttgccgtggc gcaatgtgcg gcatggcggg tggctgatgc gcagtcactc acaaaagcga
     6181 ttcaatcgct agtagcaaca ccgcatcagc aagccgaaat gggtgcaaaa ggccttgctt
     6241 tatgtcaagc gtcacagggc gctactgcgc gaacgttaaa gttgattcaa gaatatatac
     6301 gcgtagctta atttattg
//
LOCUS       DAOQSI010000123         6285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_12450_length_6285_cov_4.677368, whole genome shotgun sequence.
ACCESSION   DAOQSI010000123 DAOQSI010000000
VERSION     DAOQSI010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 6285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6285
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12450_length_6285_cov_4.677368"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..1066)
                     /gene="tal"
                     /locus_tag="PKL42_07960"
     CDS             complement(113..1066)
                     /gene="tal"
                     /locus_tag="PKL42_07960"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HNU67048.1"
                     /translation="MANLLDQLKAMTTIVADTGDVEAIKAVKPVDATTNPSLVLKASQ
                     LPQYAPLIETAIAYAKAQGGTKAQQIDNAADKLAVLIGAEITKVVPGRISTEVDARLS
                     FNLDAMVAKGRKLIKLYEESGVSKDRVLIKLASTWEGIKAGEILEKEGIQCNLTLLFG
                     FGQARACAEAGVFLISPFVGRILDWYKAKNPTTEYTQETDPGVVSVRAIYQYYKEHGY
                     KTVVMGASFRNTGELIALAGCDRLTVSPNLLQDLAATEGTLVQVLKDNGANKTPPAKL
                     TEEEFRFELNQDPMATEKLAEGIRGFVADQNKLEAALSEKL"
     gene            complement(1209..3239)
                     /locus_tag="PKL42_07965"
     CDS             complement(1209..3239)
                     /locus_tag="PKL42_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 and HATPase_c domain-containing protein"
                     /protein_id="HNU67049.1"
                     /translation="MFKKLTPLDDRLFTALVLSVIALTITLSALFTRFDYLHYDLGRY
                     LSASPAPADIVIVAIDEKSLSEIGRWPWSRTRHAMLVEKLHRAHAKVIGFDVIFAEPD
                     LNEPEADIALAQAFKVADNVVLPMLLEVPYFGAPVKLSQPTAHLLESAAGLGRVHVPL
                     DEDGIARSFYLWEGLKAEGLQAEDLAGKPAEDDLRRHFSQAVLALAGQLPANISSAPQ
                     PVHHGLFNVDFKPAASALLTQDRRYVKFLGPPGHFQRISYHQVLSGQFPVNFFKDKIV
                     LVGATAAGLGDALPTPVSALSQPMPGVEFHANAIAGMRSGNLIQHAPIWLTCIIVMML
                     SVLPLFFLPRLSPFKALCLVADYYLAVTFVAIALPQYLNVWVPPAGALIAILLTYPIW
                     SWRKLESAQIYLDNALKELQAELGQLGVEKANLESIQARDEMQSRIAKVEITSQFLRE
                     SQQKRLDTLAFISHDIRAPLAAAIMQLEEVENATSSKSALRVTQMLNRALNMADEFLQ
                     SSRAEMVDTSKFSDIQVNGLLQETLDDAYTLAQSNHIKLNASLSDEPMWIQGNFGLLQ
                     RAIANILSNAIKYSQPNTAVQIVLSKVDDEMVLNITDTGPGIPPEKVSKLFKRFSRVE
                     GEYQLPVGTGLGLYFVDVTIQKHHGRIFVESMLNEGTTFTIRLPLVATQFEE"
     gene            3262..3507
                     /locus_tag="PKL42_07970"
     CDS             3262..3507
                     /locus_tag="PKL42_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67050.1"
                     /translation="MKFFESHQFGNSTRKNDQTILYIVAKNFTPENHVFQPQNVREKN
                     DALEAFIYKASRASKKVATKNQLKTTMMNGYFALLLT"
     gene            complement(3499..4884)
                     /locus_tag="PKL42_07975"
     CDS             complement(3499..4884)
                     /locus_tag="PKL42_07975"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNU67051.1"
                     /translation="MNAVNSMKHFITYVVVFCSLMCGYSAFAEEHATETAWHYTVKPN
                     DSFELIYRKYLATRAGIAKLSSHNQHPLSKKLQPGKVLIIPLALLKKTPANVQVLVAS
                     GDVMASSAQQQTPRAIKVGDALTQGDKLKTGKCSVAKLRFADGSVVDVQQNSSIEIHA
                     SYQYAGKETFVSLLKLNNGRTEIEANPNRTIGNSMQIETPSAIAAVRGTQFRVATGAD
                     IALQETIEGQVAFSAAGQEVLLAKGYGSVVEKNQAPLPPIVLPDSPDVSGFKQQFEQN
                     HAAFNLVPQPEVVAFVSQLARDAEFTQIVHEHVTQAEQNATLAITDLADGQYYLRLRA
                     QESHGLQGQSAVHSFTVKARPQAALELLAPTAAAQIAETPLTFAWTPNPNNAIYTLQI
                     ARDADFQNKVYETMTTLTQVTLAPAFAHAQLTQGEYYWRVISTPNLLKNQPQKANPQD
                     KMISKVGKILR"
     gene            complement(4868..5566)
                     /locus_tag="PKL42_07980"
     CDS             complement(4868..5566)
                     /locus_tag="PKL42_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNU67052.1"
                     /translation="MVKKARIAFLEDDSTYAGLIVEWLSSAGFEVKLFTLSLDFLRNF
                     SDAQYDLCLFDFMMPDMSGLDVMGSIKLRSKKAPPIVFLTARSDEEDIVHVIESGADD
                     YIVKPVSRPVLLARINALIRRSSTEHKNNIVSEFGALKIDSQQRLVTIDGQEVKLTDK
                     EFDLALYFLRNEGALLSRVHLMQVVWGSSSDVETRKVDVHVSHLRTKLKLTPEYGWKL
                     TSIYQQGYRLERSQ"
     gene            complement(5741..>6285)
                     /locus_tag="PKL42_07985"
     CDS             complement(5741..>6285)
                     /locus_tag="PKL42_07985"
                     /inference="COORDINATES: protein motif:HMM:NF013991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HNU67053.1"
                     /translation="QDVLHHLSVTDAEDASSAIVLANPAGLGEVSQYDVHAQPQVTLL
                     ALMQAAENHDRIAWQYSHNFSDIFNVGIPRFQAGLQRWQHFNNKGQNLAWATTAVYLG
                     FLARQPDTHIVRKHGHTIATEVMMQAKLREASFWQSENPKLQQSELLAWDTALKDQKL
                     NPGTSADLTVATLMVHNLTN"
BASE COUNT         1736 a         1483 c         1262 g         1804 t
ORIGIN      
        1 aaaaactcta aaactacaat gcttgttgag acaagactga ctacaaaaat gcaggacact
       61 tataataaat gtcctgctag tctgtgtcaa ttctaaatta gatgaatgcg ccttaaagtt
      121 tttcgcttag cgctgcttct agtttgtttt ggtcagccac aaaaccacga ataccttcag
      181 ctaatttttc tgtagccatt gggtcttggt ttaattcaaa gcggaactct tcttcagtga
      241 gtttagcagg tggtgttttg tttgcgccat tgtcttttaa cacttgtacc aaggtacctt
      301 cagtagcggc caaatcttgc aacaagttag gcgaaacggt taagcggtca cagcccgcta
      361 aagcaattaa ctcacctgtg ttacggaatg aagcacccat cactactgtt ttgtagccat
      421 gctctttgta gtattggtaa atagcacgca ctgaaaccac gcctgggtct gtttcttgtg
      481 tgtattctgt tgttgggttt tttgctttgt accagtcaag aatacggccc acaaatggtg
      541 aaatcaagaa tacgcccgct tcagcgcaag cacgtgcctg accaaaacca aataacaatg
      601 taaggttaca ttgaatgcct tctttttcaa gaatctcacc ggctttgatg ccttcccaag
      661 ttgaggctaa tttaatcaac acgcggtctt tgctcacacc tgactcttcg taaagtttga
      721 ttaatttacg gcctttggcc accatagcgt ctaagttgaa agacaaacgt gcgtccactt
      781 cagttgaaat acggccagga acaactttcg taatttctgc accaattaac acggctaatt
      841 tgtcagcagc gttgtcaatt tgttgtgctt tagtaccacc ttgtgctttt gcataggcaa
      901 tcgcagtttc aattaaaggt gcgtattgtg gcaattggct tgcttttaac actaatgatg
      961 gattagtggt tgcatccact ggctttactg ctttaatcgc ttcaacatcg cctgtgtctg
     1021 ccacaattgt tgtcatcgct ttgagttgat ctaataaatt tgccatactg cctcctgaaa
     1081 aataatcgca ctgaaatgtt ttaaaatttt agcttgtaaa tttttataaa atttagctgg
     1141 tgattataac ttaaataaaa ctggttgtca gtgccagcgc ggcaacttgc gccatttcgt
     1201 agcattattt actcttcaaa ttgtgtagct accagcggca gcctaatggt aaatgttgtg
     1261 ccttcattca acatgctttc aacaaaaata cgaccgtgat gtttttgaat ggtcacatcc
     1321 acaaaatata ggcctaaccc agtgcctaca ggtaattggt actccccttc aacgcggctg
     1381 aagcgtttaa acaatttact cactttttct ggcgggatgc ctggcccggt gtcggtgatg
     1441 tttaacacca tttcgtcatc cactttacta agaacaatct gcaccgcggt gtttggctgt
     1501 gaatacttaa tggcattgga taatatgttg gcaattgcgc gttgtaaaag gccaaagtta
     1561 ccttgaatcc acatcggctc atcacttaaa ctcgcattga gcttgatgtg attagattgc
     1621 gcaagggtgt acgcatcatc cagcgtttct tgcagtaagc cattcacctg aatatcgcta
     1681 aacttgctgg tatccaccat ttctgcgcgg gaagattgca aaaactcatc ggccatattc
     1741 aaggcacgat tgagcatttg cgtcacacgc aatgctgatt tgctagaagt ggcattttct
     1801 acttcttcca actgcataat tgctgcggcc aaaggcgcgc gaatgtcatg tgaaataaac
     1861 gccagcgtat ctaatctttt ttgctgtgat tcgcgcaaaa attgcgaagt aatttcaacc
     1921 ttggcaatgc gtgattgcat ttcatcgcgc gcttgaatag attctaaatt tgccttttca
     1981 acccccaatt gcccaagttc tgcttgcaac tcttttaggg cattatccaa ataaatctgt
     2041 gctgactcca gcttgcgcca actccatatc gggtaagtta gcaatatggc aatcaatgcg
     2101 cctgctggtg gcacccacac atttaaatat tgcggtaagg caattgccac aaaagtcacg
     2161 gcaaggtagt agtccgccac caaacatagc gctttaaatg gactcaatcg aggtaaaaaa
     2221 aagaggggca acactgacaa catcatgaca atgatacaag taagccaaat gggtgcatgc
     2281 tgaattaaat tgccactacg catgccggca attgcattgg catgaaactc aacgcccggc
     2341 atgggttgcg acaaggctga aacgggtgtt ggcagtgcat cccctaaacc agcggcggtt
     2401 gcccccacca gcacgatttt atctttgaaa aaattaactg gaaattggcc actcagcact
     2461 tgatggtacg aaatacgctg aaaatgtcct ggtgggccta aaaacttcac atagcgccta
     2521 tcttgtgtaa gtaacgcact ggcagcgggc ttaaaatcta cattgaataa tccatgatga
     2581 actggctgtg gtgcgctcga tatattcgcc ggcaactgcc ctgccagcgc caatactgct
     2641 tgcgaaaaat gtcgtcttaa atcgtcttcg gctggttttc ccgctaaatc ttccgcctgc
     2701 aagccctccg cttttaagcc ttcccaaagg taaaaactgc gggcaatgcc atcttcatct
     2761 aacggcacat gtacgcggcc taaccccgct gcagattcaa gcaaatgtgc tgttggttgg
     2821 cttaatttaa ctggcgcacc aaaatatggc acttccaaaa gcattggcag caccacattg
     2881 tctgcaactt taaacgcttg cgccaacgcg atatctgcct ctggttcatt aagatcaggc
     2941 tctgcaaaaa tcacatcaaa tccaatcact ttggcatgcg cccgatgaag cttttccacc
     3001 agcatcgcat ggcgcgtgcg cgaccatggc caacggccaa tctcactcaa acttttttca
     3061 tcaatcgcca caatcacaat atctgccggt gcaggcgaag cagaaagata acgacctaaa
     3121 tcgtaatgca gataatcaaa acgtgtgaac agcgcactta atgtaatcgt cagcgcaatt
     3181 actgacagca caagtgccgt gaacaagcgg tcgtctaatg gcgttagttt tttaaacatc
     3241 tcgcgttaag cataattgtt tttgaaattt ttcgaaagtc atcagtttgg caattcaaca
     3301 cgaaagaatg atcagacaat actctacatt gtggctaaaa atttcacgcc tgaaaatcat
     3361 gttttccagc ctcaaaatgt acgagaaaaa aatgatgctc tggaagcctt tatttataag
     3421 gcttccagag catcaaaaaa ggttgcaaca aaaaatcagc taaaaacaac catgatgaat
     3481 ggttattttg cgctattgct aacgtaaaat cttccctact ttagaaatca ttttgtcttg
     3541 gggattagcc ttctgaggtt gatttttcag cagatttggc gtagatatca cacgccaata
     3601 atattcacct tgcgtgagtt gcgcgtgcgc aaatgcaggt gctaatgtaa cctgtgtcag
     3661 ggtcgtcatc gtctcatata ctttgttttg aaaatcagca tctcgggcga tttgcaaagt
     3721 atagatcgca ttgttagggt tgggcgtcca cgcaaaagta agcggcgttt cagcaatttg
     3781 tgcagctgct gtaggcgcca ataattcaag cgcggcttgt ggtcgcgctt ttacagtaaa
     3841 gctgtgaact gcactttggc cttgtagccc gtgtgactct tgcgctctaa gccgcaaata
     3901 gtattggcca tcggccaagt cagtaatcgc caatgttgca ttctgttctg cctgcgtcac
     3961 atgttcatgc acaatctggg taaattccgc atcgcgcgcg agctggctta caaaggcaac
     4021 cacttcaggt tgcggcacta aattaaacgc cgcatgattt tgctcaaact gttgtttaaa
     4081 accactgaca tcgggtgaat caggcaacac aatcggcggc aaaggcgctt gatttttttc
     4141 aaccacactg ccgtaaccct tcgccaacaa cacctcttgc cctgctgcag aaaaagcaac
     4201 ttgtccttct atggtttctt gtaatgctat atcagcacct gttgctaccc taaactgcgt
     4261 gccacgcacc gccgcaatgg ccgatggcgt ttcaatttgc attgagttac caatggtgcg
     4321 gtttggattc gcttcaattt ccgtacgccc gttattgagt tttaacaaac tcacaaacgt
     4381 ttctttgccg gcatattgat agctggcatg aatttcaata ctcgaatttt gctgcacatc
     4441 caccactgag ccatcggcaa aacgtaattt tgccacgcta catttgcctg ttttaagttt
     4501 atcgccctgc gtcaaagcgt cacccacttt aatagcgcgt ggcgtttgct gttgtgctga
     4561 actggccatc acatcacctg aagccaccaa cacctgaaca ttcgcaggcg tttttttaag
     4621 cagcgctagc ggaataatca acaccttccc tggttgcagt ttttttgaca gcggatgctg
     4681 attgtgtgaa gacagcttag cgatgccagc gcgcgttgca aggtacttac ggtatattag
     4741 ctcaaatgaa tcattgggtt ttaccgtgta atgccaagcg gtttcagttg catgttcttc
     4801 tgcaaaagcg ctgtaaccgc acatgagcga acagaaaacc accacataag taataaaatg
     4861 cttcatgcta ttgactgcgt tcaagacgat acccttgctg ataaattgag gttaatttcc
     4921 acccatattc aggggttaat tttagtttag tacgcaaatg gctcacatgt acatctactt
     4981 tacgtgtttc aacatctgaa ctcgagcccc aaaccacttg cattaagtgt acgcgtgaaa
     5041 gcagcgcacc ttcatttctt aaaaagtata aggctaagtc aaactctttg tcggtcagct
     5101 tcacttcttg accatcaatc gtcactaagc gctgttggct atctattttt aaagcaccaa
     5161 attcactgac aatattattt ttatgttctg tactggaacg tctaatcagt gcattgatac
     5221 gagcaagcaa caccgggcga cttactggct taacaatata atcatctgca cctgattcaa
     5281 tgacatgtac gatgtcttct tcgtcactac gtgcagtcaa aaaaacaatg ggtggcgctt
     5341 tcttagatct aagcttaatg cttcccatca catctagacc gctcatatcc ggcatcataa
     5401 agtcaaacaa gcacaaatcg tactgcgcat cagagaagtt gcgcaaaaaa tccaggctca
     5461 aagtgaagag tttaacttca aatcccgccg aacttagcca ctcaacaatc aaacctgcat
     5521 aagttgaatc atcttctaaa aaagcgattc ttgccttttt taccatctcc aaaatacgcc
     5581 tacctttact tatgatgttt taaattaact actggcttac aattggccaa taaccctaaa
     5641 cctagcagta atatcacaaa ttatgtttag ggtactaaaa tttaacaatt ttttacataa
     5701 aaaatcatca ataaaaatag atattaatat tttaaatcaa ctagttggtt aaattatgta
     5761 ccatcagcgt tgccactgtc aaatctgcac tcgtgcctgg atttagcttt tgatctttga
     5821 gcgcagtatc ccacgccaac aattcactct gttgtagttt tgggttttca gattgccaga
     5881 aagacgcctc tcgcagcttc gcctgcatca tcacttccgt cgctattgta tggccatgtt
     5941 tacgaacaat atgggtatct ggctgccgtg ccaaaaagcc taaataaact gcggttgttg
     6001 cccatgccag attttggcct ttgttattaa agtgttgcca gcgctgtagg ccagcctgaa
     6061 aacgcggaat acccacgtta aaaatatccg aaaaattatg ggaatattgc caagcaatgc
     6121 ggtcatgatt ttccgcagcc tgcatcaatg ccaagagcgt tacctgtggc tgagcatgca
     6181 catcatactg gctcacttca cccaagcctg ccgggttagc taaaacaata gcgcttgacg
     6241 cgtcttctgc atctgtaaca cttaaatgat gcagcacatc ttgca
//
LOCUS       DAOQSI010000124         6137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_12890_length_6137_cov_5.578757, whole genome shotgun sequence.
ACCESSION   DAOQSI010000124 DAOQSI010000000
VERSION     DAOQSI010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 6137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6137
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12890_length_6137_cov_5.578757"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..139
                     /locus_tag="PKL42_07990"
     CDS             <1..139
                     /locus_tag="PKL42_07990"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184012.1"
                     /note="bifunctional enzyme DHBP synthase/GTP
                     cyclohydrolase II-like protein; functions in riboflavin
                     synthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNU67054.1"
                     /translation="TYGIGSQILLDCGVRKMKLLANPRKMPSMAGFGLEVTGYLEANT
                     Q"
     gene            181..765
                     /locus_tag="PKL42_07995"
     CDS             181..765
                     /locus_tag="PKL42_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNU67055.1"
                     /translation="MPLENINLTGHFLIAMPAMTDSFFSKSVTFICTHNQDGAMGIMI
                     NRPTDITFETLFEKINVELLNIGVADKPVLYGGPVQPERGFVLHEITDGEWDSTVQIA
                     NNIALTTSKDILESVAIGHGPAKMLVTLGYAGWTSGQLEQEIMQNAWLSVQANDTETL
                     HKILYDTDYDEKLSATLALLGVDPAMLSDVAGHA"
     gene            765..1265
                     /gene="ruvX"
                     /locus_tag="PKL42_08000"
     CDS             765..1265
                     /gene="ruvX"
                     /locus_tag="PKL42_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNU67056.1"
                     /translation="MMLDTEINATTTHLLIDNENVYDAHAVLKSTVLCFDFGEQRIGV
                     AVGEHMLASANPLTTISSESNDVRFEVIGKLINEWQPKLLVVGLPLNVDGTAHNTTQL
                     CKKFARRLNGRFNLPVMLVDERYSSIEASEKLNDAGIKGRAQREMLDQVAAQGILQSY
                     FNRIQT"
     gene            1313..1843
                     /gene="pyrR"
                     /locus_tag="PKL42_08005"
     CDS             1313..1843
                     /gene="pyrR"
                     /locus_tag="PKL42_08005"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNU67057.1"
                     /translation="MTSKKTALPDAEALLLTLAEKIKPMLQPNTALVGIQSGGVWLMQ
                     RLLAMLESTIAANNVEHGTLDVSFYRDDYAQRGLKIETRPSQIAFDVEGKHIILIDDV
                     FYTGRTTRAAMNELFDYGRPASISLVALINRGGRELPITPQITAADIVLDTHQNLQLI
                     QNANGILSLELQTSSE"
     gene            1852..2817
                     /locus_tag="PKL42_08010"
     CDS             1852..2817
                     /locus_tag="PKL42_08010"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNU67058.1"
                     /translation="MNNPQLDADGRLKHLLSIEGLPKKILNQILDTAESFVGVAEREV
                     KKVPLLRGKTVCNLFFENSTRTRTTFEIAAKRLSADVISLNVNASSQSKGETILDTIN
                     NLIAMHADMFVVRHAQSGAAHFIAQHVPSHIHVINAGDGRHSHPTQGLLDVFTIRKYK
                     PELHNLRVAIVGDVLHSRVARSEIHALTTLGVPEIRVIAPKTLLPTQVEKLGVHVYHD
                     INTGLKDVDVVMMLRLQNERMNGAMLPSVQEYFKTYGLTQERLALAKPDAIVMHPGPM
                     NRGVEIDSSVADGQQSVILPQVTYGIAIRMAVMAILAGGQAGGTQ"
     gene            2814..4100
                     /locus_tag="PKL42_08015"
     CDS             2814..4100
                     /locus_tag="PKL42_08015"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNU67059.1"
                     /translation="MNIVIKNGRVIDPKNNIDKQLDVYVAAGKIAGVGVAPTGFIANQ
                     TIDAANLVVCPGLVDLSARLREPGDEHKARLISELKAAVAGGVTSLACPPDTDPVLDE
                     PGLVEMLQYRAKQQGLANVYPLGAITRQLHGKALAEMSELQTAGCVGFSQANIAITDT
                     QVLWRAMEYAATFGYTLFLHAEESFLAANGVAHDGEVATRLGLKGIPSAAEALALASI
                     LRIAQETGAKVHISRLSSAEGVAMVRTAKSQGLNITCDVSANHLHLTEHDIGYFNTHC
                     HLKPPLRTQRDKAALCLGLKDGTIDAVCSDHSPVDDDAKLTTFADAEIGATGLELLLP
                     LTLKWAQQEKLNLSAAIASITCTPANILGIAAGNLSINSVADICIFDANAYWNVNVNT
                     IKSQGKNTPFLGWELAGKVITTIVNGQIVHTRSLDD"
     gene            4264..4722
                     /locus_tag="PKL42_08020"
     CDS             4264..4722
                     /locus_tag="PKL42_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67060.1"
                     /translation="MSGFTCCFWWFLLGFVLGWLLNWLLKRCCGQCNTQQAAPVVNAV
                     AAAPAAPKVIPFDLAAAKAAGFNIRKADDLTVVEGIGPKINELLLNDGIKTFAQLAAT
                     NVSVIQAILDKAGPSFALAKPGTWPQQAALAAENRWAELKVLQDNLKGGV"
     gene            4780..5553
                     /locus_tag="PKL42_08025"
     CDS             4780..5553
                     /locus_tag="PKL42_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNU67061.1"
                     /translation="MSNSKYNFWTWLVAIVLAIYLFWQWQHGHGLGSAAGCCGATVAE
                     VPAAPVAAPEAVAPEAFQFTADNEKFQSSGNADSVAWYAKNVDLVSWLKAGGADWQAS
                     GNDHSVTLTGTVANEEAKTQAGKDAQAFFGENIAIDNQLVVKAADPVAAIAPPPAAKL
                     YFDTGKTDLANDAEQTLAPIVEWLKANASAKAIISGYHDPRGNLAVNEELAKNRAKAV
                     RDALKAAGIDEARIELRKPVSTEGDGDLAEARRVEVSVE"
     gene            complement(5792..>6137)
                     /locus_tag="PKL42_08030"
     CDS             complement(5792..>6137)
                     /locus_tag="PKL42_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HNU67062.1"
                     /translation="LNTRAFISQRLIPKKEGKGRVAAMEVMLNSPLISDLIFKGDVHE
                     IKEIIAKSRELGMQTFDQSLFDLHEADIITYEDALRNADSVNDIRLKIKLEGKAAHGK
                     DLGAGLDGLGIV"
BASE COUNT         1763 a         1430 c         1408 g         1536 t
ORIGIN      
        1 cacctatggc ataggctcac aaattttatt agactgcggc gtacgtaaaa tgaaactact
       61 tgctaacccg cgcaaaatgc ccagcatggc aggcttcggg cttgaggtaa cgggttattt
      121 agaggcgaat acccaataat tgcgatatca ctttaaaaat caattcacta tgttagaata
      181 atgccattgg aaaacatcaa tctcaccggt cactttctca tcgcgatgcc cgccatgacc
      241 gattcttttt tcagcaaatc cgttaccttc atctgcacgc acaatcaaga cggtgcgatg
      301 ggcattatga ttaaccgccc gaccgacatt acctttgaga ctttatttga aaaaatcaac
      361 gtagaattac tgaatattgg cgttgcagat aagcctgtgt tatatggtgg gccagtacag
      421 cctgagcgcg gttttgtgct gcacgaaata acggatggcg agtgggatag caccgtgcaa
      481 attgcaaaca atatcgcgtt aaccacttca aaagatattt tagaatcggt agccatcggc
      541 cacggcccgg caaaaatgct ggtcacttta ggttatgcag gttggacttc agggcaactt
      601 gagcaggaaa ttatgcaaaa tgcatggtta tcggtacagg caaacgatac tgaaacacta
      661 cacaaaattt tatacgatac cgattatgat gaaaagctat ccgctacttt agcgttattg
      721 ggcgttgacc ctgcgatgct ttctgacgta gcgggtcacg cttgatgatg ttggatactg
      781 aaatcaacgc gacaaccacg cacttgttaa ttgacaatga gaacgtgtac gatgcccatg
      841 ctgtgcttaa aagtaccgtg ctgtgttttg attttggaga gcaaagaatc ggcgtagccg
      901 ttggcgaaca tatgttagca agcgccaacc cactcaccac catcagcagc gaaagtaacg
      961 atgtccgctt tgaagtcatc ggcaaattga tcaacgaatg gcaacctaag ctactggtgg
     1021 ttgggttacc tttgaatgta gatggtaccg cacacaacac aacgcaattg tgtaaaaaat
     1081 tcgcaagacg cctgaatggc cgctttaatt taccagtgat gttagtggat gagcgttaca
     1141 gctcgatcga ggccagcgaa aaacttaacg acgctggcat caaaggccgc gcgcaaagag
     1201 aaatgctaga tcaggtcgcg gcacaaggca tcttacaaag ttattttaat cgcattcaaa
     1261 cttgaattat gataacaaca atacttttga taaaaatgta cgcatacgca aaatgacctc
     1321 aaaaaaaacc gcactgcctg atgcagaggc tttattgctc acccttgctg aaaaaatcaa
     1381 gcctatgctg cagccaaaca cggcgctggt cggtattcag agtggcggcg tttggctcat
     1441 gcaaaggctg ctagccatgc tagaaagcac cattgctgca aataatgtcg aacatggcac
     1501 attagatgtc tctttttatc gggatgatta cgcacagcgc ggcttgaaaa ttgaaacacg
     1561 cccaagccag attgcctttg acgttgaggg aaaacacatc attttgattg atgatgtatt
     1621 ctacaccggc cgcacaacgc gcgccgccat gaatgaattg tttgattacg gccgccctgc
     1681 aagcatcagt ttagttgccc tgattaaccg tggcggtaga gaactgccca ttaccccaca
     1741 aattactgca gcagacattg ttttggatac ccatcaaaac ctgcaactca ttcaaaatgc
     1801 taatggcatt ttaagccttg aactccaaac ttcttcagag tgaacacgcg catgaacaac
     1861 ccgcaattag atgctgacgg ccgcctgaaa caccttctct ctatcgaagg gttgccgaaa
     1921 aaaattctca atcagatttt agacaccgcc gaatcttttg tgggtgtagc agaacgtgaa
     1981 gtgaaaaaag taccgctatt acgcggaaaa accgtgtgta acttgttttt tgaaaacagc
     2041 acccgaactc gcaccacttt tgaaatcgcc gctaaacgcc tctcagccga tgtgattagc
     2101 ctaaatgtga atgcatcatc ccaatccaaa ggcgaaacta ttttggatac catcaataat
     2161 ttaattgcca tgcatgcgga catgtttgtg gtgcgacatg cgcaatcggg cgcagcacac
     2221 tttattgctc aacatgtgcc ttcacatatt cacgtgatta atgccggtga tggtcgtcat
     2281 tcacacccaa cacaaggctt gctggacgta tttactattc gtaaatataa gcctgaatta
     2341 cacaatttac gcgtagcgat tgtgggtgat gtattgcact ctcgcgtagc gcgcagtgaa
     2401 atccacgcgc tcacaacact gggggtgcct gaaattcgcg tgattgcgcc taaaacctta
     2461 ttacccacac aagtagaaaa actaggcgta catgtttacc acgacatcaa caccggctta
     2521 aaagatgttg atgtggtgat gatgctgcgc ctgcaaaacg aacgcatgaa tggcgccatg
     2581 ttgcctagcg tacaagagta ttttaaaacc tacggcctca cccaagaaag actcgcgctt
     2641 gcaaaaccag atgccattgt gatgcaccca ggcccgatga accgcggtgt ggaaatcgac
     2701 tcaagcgttg ccgatggcca gcagtctgtt atcttgcccc aagtcacgta cggcattgcc
     2761 attcgcatgg cggtcatggc catattagca ggcggtcaag cgggaggaac gcaatgaata
     2821 ttgtcatcaa aaatggtcgc gtgattgacc caaaaaacaa tattgataag caactagatg
     2881 tctatgttgc agcaggtaaa attgccgggg tcggcgtagc ccccacaggt tttatagcaa
     2941 accagacgat tgatgcagcc aacttagtgg tttgccctgg tctggttgac ttgtctgcca
     3001 gacttcgcga accaggtgat gaacataaag cgaggctaat cagtgagtta aaagctgccg
     3061 ttgctggcgg cgtgaccagt ttagcctgcc cgcccgatac cgaccctgta ctcgacgagc
     3121 cgggcttggt tgaaatgttg caataccgag cgaaacaaca agggctcgca aatgtgtatc
     3181 cgctaggagc cattacgcga caactgcatg gcaaggcctt ggctgaaatg agcgaactgc
     3241 aaaccgctgg ttgcgttgga ttttcacaag ccaacattgc cattactgat acgcaagtgc
     3301 tgtggcgagc catggaatat gcggctacat ttggctatac cttattttta catgcggaag
     3361 aatccttttt agctgccaat ggtgttgcac acgacggcga agtcgcaacg cgccttggcc
     3421 tcaaaggcat acctagtgcc gctgaagcac ttgcattggc cagcattttg cgcattgccc
     3481 aagaaactgg ggcaaaagtg catatcagca gactttcatc ggcagaaggc gttgctatgg
     3541 tacgcactgc aaaatcgcaa gggctcaata tcacttgcga tgtcagcgca aaccacttgc
     3601 atctgaccga acatgatatt ggttacttca atacccattg ccacctcaaa ccaccactac
     3661 gcacgcagcg tgataaagct gcgctctgcc ttggcctgaa agacggcacg atagatgctg
     3721 tctgctcaga ccactcccct gtggatgacg atgccaaact gaccacgttt gctgatgcgg
     3781 aaatcggggc aaccggcttg gaactattac tgccgctcac gctgaaatgg gcgcaacaag
     3841 aaaaattaaa cctctcagct gcaatcgcca gcatcacttg cacacctgcc aatattttgg
     3901 gcattgctgc gggaaattta agcatcaata gcgttgctga tatttgcatc tttgatgcca
     3961 atgcctattg gaacgtcaat gtaaacacca tcaaaagcca aggcaaaaac acgccatttt
     4021 taggctggga actcgctggt aaagtcatca ccactatcgt caatgggcaa atcgtacata
     4081 cacgatcact cgatgattaa aatgtaaagt atcgtacagc atattgcctt tcaattatgg
     4141 caggtgcaaa atgcgctttg taatgtgcag atgtgccggt aatttataat gaatgttgcc
     4201 gcacaaaata aaaaaagttt acattttttg gtcgttttaa tttagcctga tagggagaga
     4261 aaaatgagtg gatttacttg ctgtttttgg tggtttttat tagggtttgt tctcggctgg
     4321 ttactcaact ggttattaaa acgttgctgc ggccaatgca atacccaaca agctgcaccc
     4381 gttgttaacg ccgttgcagc cgcacctgct gcgccaaaag tcattccatt tgatttagca
     4441 gccgccaaag cagccggctt taatatcaga aaagcagatg acctaacagt tgttgagggc
     4501 atcggcccaa aaattaatga actactctta aacgatggca ttaaaacctt tgcacaattg
     4561 gcagcaacta atgtatctgt cattcaagcc attttagata aagcaggacc aagctttgcg
     4621 cttgcaaaac caggcacctg gccacagcaa gctgcacttg ccgctgaaaa tcgttgggct
     4681 gaactgaaag tattacagga caacctcaag ggtggcgttt aatgccgaaa tattacggct
     4741 aacgtcaatc accattaacg atttgaagga gcgagttcta tgagtaactc aaaatataat
     4801 ttctggacat ggctggtagc cattgttctg gccatttatt tattttggca atggcagcac
     4861 gggcatggtt taggctcggc tgctggttgc tgcggcgcca cagtagctga agtacctgct
     4921 gcaccagtgg ctgcacctga agccgtagca cctgaagcgt ttcagttcac tgcggacaat
     4981 gaaaaatttc aatcttctgg caatgcagac agtgtggctt ggtatgctaa aaatgttgat
     5041 ttagtcagct ggctaaaagc tggtggcgca gattggcagg caagtggtaa cgaccattct
     5101 gtaacgttaa ctggcacggt tgcaaatgaa gaagccaaaa cgcaggcagg caaagatgcg
     5161 caagcatttt ttggtgaaaa tattgcgatt gacaatcaac ttgtggttaa agccgcagat
     5221 ccagttgccg ccattgcacc tccacccgca gctaaacttt actttgacac aggcaaaacc
     5281 gatttagcca atgatgcgga gcaaacactt gcgccgattg tagaatggtt aaaagccaat
     5341 gcaagcgcta aagcgattat ttctggctac cacgacccgc gtggcaatct ggccgtgaat
     5401 gaggaactgg ctaaaaaccg tgcaaaagcg gtccgtgatg cactgaaagc agcaggcatt
     5461 gacgaagcgc gcattgagtt gcgtaaacct gtaagcactg aaggtgacgg cgatcttgct
     5521 gaagcccgcc gtgtggaagt ttctgtagaa taaaattcta cagattaaaa aataaaaagg
     5581 ccgatgttaa catcggcctt ttttatggcg gtaagcttgc tcaaaacggc ggccaacata
     5641 gcgtttaaaa aaacataaaa acagcctatt ttaacaacaa cctttttcga tgctctgcga
     5701 gccttgtaaa taaaggctcg cagagcatct ttttttcctc gggcgtaaat tggtgtgcat
     5761 taacgcaaaa gaataagatg tttatttaaa attacacgat gcctaaacca tcaagtccgg
     5821 cacccaaatc tttaccatgc gcggctttgc cttcaagttt aattttcagg cggatatcgt
     5881 tcacagagtc ggcatttcgt agtgcatctt cgtaggtaat aatatcggct tcatgcaaat
     5941 caaacaaaga ttggtcaaag gtttgcatgc ccagttcacg tgattttgca ataatttctt
     6001 taatctcgtg cacgtcacct ttaaaaatca agtcagaaat caaaggcgag ttcagcatca
     6061 cttccatcgc agcaacgcgg cctttaccct cttttttagg gattaaccgt tgcgagataa
     6121 atgcgcgcgt attcaga
//
LOCUS       DAOQSI010000125         5911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_13642_length_5911_cov_4.110656, whole genome shotgun sequence.
ACCESSION   DAOQSI010000125 DAOQSI010000000
VERSION     DAOQSI010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13642_length_5911_cov_4.110656"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..1514
                     /locus_tag="PKL42_08035"
     CDS             36..1514
                     /locus_tag="PKL42_08035"
                     /inference="COORDINATES: protein motif:HMM:NF037202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67063.1"
                     /translation="MQFNSNSSFIDGLIHRQMRKSLLESFDEIYILDLHGNAKKQETS
                     PDGSKDENVFDIMQGVSINLFIKTGKKAKGSLGQVFNHSLYGKREEKYNALNDGEISS
                     LPWQKLDVIAPDYFFSVKNFDNTDLYKNGFSVIELFSLIGSGISFRKDSLLIRQNDTK
                     KDVYQMIDDIKSLDPSILESKYSFKQSIDWKVIEQRQNFLNPLNDDIKQVSYRPFDSK
                     YAYYPLEKISQIIPRGDSRKNIMQHMLKENLTLIFERSVTIGDFTNIFISNKLVEAHV
                     LGTMFSKGYVAPLYIYPDASDLLAEVSRIPNLNMQIVETISKGLNLTFTREKEDLTNT
                     IAPIDLLDYIYAVLHSPTYRETYKEFLKIDYPRVPYPSDEEQFFQLVALGGELRQLHL
                     LEGAAINQLITSYPESGNNLVDKPRYKNSKVYINDTQYFDGVPKLAWEFYIGGYQPAQ
                     KWLKDRKGRILSYEDILHYQKIIKAMVETDRLMHEIDKFKIV"
     gene            1858..3195
                     /locus_tag="PKL42_08040"
     CDS             1858..3195
                     /locus_tag="PKL42_08040"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HNU67064.1"
                     /translation="MKWLKFTGIIFLLLLAVLAALPFFISLNDYIPLIENKLSAKIDE
                     PVTINNIQLRLLPTPHINVNDVMVGVEGDLKLARVVLTPDIFSIFKSSYLIKDIEIDA
                     LVINQKAINRLMKFTKEASSPPHAKPQFRVGRLSFTNAQLNLGKLHFGPFHANVNLDS
                     NGKLLEASLATVDSKFRALIKPENLNYSIVASAKNWTLPYEPRFNLDELSLLAVATTN
                     DIQFSKVNAKLYGGTAKGQMAINWRKGLKVNGSLDVRQVELEKIAKILSSKTHVSGQV
                     TAKPVFSASANSIENLMQSMRLEASFDVQKGVLYGVDIQSAATNVIKQGTTGGQTSFE
                     HLSGYFVMAKSSYQFSKLKITSGTLSVEGNVNISHKKELSGRINTQVKVAGISTIVPL
                     NVAGTLDAPRLHPTAATMAGAALGTAVMGPGVGTSVGAKVGGWVDDLFGKKKK"
     gene            3294..4379
                     /locus_tag="PKL42_08045"
     CDS             3294..4379
                     /locus_tag="PKL42_08045"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNU67065.1"
                     /translation="MGEIVPTERDVPQDDDWGTIGHVQTLVLMMVTVLGIYLCYKMAL
                     PFLAVVAWALTLAVLFTPLQNWLEAKLKRTGLAAFISILLIGVIVVIPTLFVGQQLVI
                     QAVKGSQIVEQKINSGEWRRSLEAQPQLAPIIDRVEKYVDLPGTLKIFTTWLGSAAGA
                     IVKGSVYQVLGICLIFYMLFFFIRDRRLALAAIKALSPLSENEMDTMIRRIGDTIHAT
                     VYGTFAIAAVQGLLGGLMFWWLDLPAPFLWGLVMSLLAIVPMLGASIIWAPAALFLAL
                     DGNTSSALMLTIWGVFVVSTIDNLLRPIFVGNRLKLHTVLAFMSVVGGLLVFGAAGLI
                     LGPVTLTITITLLDIWAKRTAVKTTDA"
     gene            4372..5244
                     /locus_tag="PKL42_08050"
     CDS             4372..5244
                     /locus_tag="PKL42_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNU67066.1"
                     /translation="MHNVQQENDCCLSLEMFNGDLPQRHANAHKGTFGSVAVIGGATG
                     MVGSVLLAARAALHSGAGRIYAALLSENAPAVDFIQPEIMLRALDDIVQLPQLNCLVI
                     GPGLGQSMVAIKQLEYWVNQSVQILLDADALNLIASHPHLASAVASRTSPTVMTPHAG
                     EAGRLLAFSTEAVTQNRLESAIILAEKFNAICVLKGAGTLIVQDKHHYFINTTGNVGL
                     ASGGTGDVLSGIIGSLMAQGLAAINAAKLGVFVHGAAADALVKKGIGPIGLTASEVIL
                     EVRQQLNQLHKNTA"
     gene            complement(5337..5855)
                     /locus_tag="PKL42_08055"
                     /pseudo
     CDS             complement(5337..5855)
                     /locus_tag="PKL42_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831783.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
BASE COUNT         1758 a         1092 c         1346 g         1715 t
ORIGIN      
        1 gtcaatttta aatgcaattg gtgggtcaat tattaatgca attcaacagt aatagtagct
       61 ttattgatgg tttaattcac cgccaaatgc gtaaaagcct actagaaagt tttgatgaaa
      121 tttatatatt agacttgcat ggtaatgcaa aaaaacaaga aacctcacca gatggcagca
      181 aagacgaaaa cgtgtttgac atcatgcaag gcgtgagcat caacctgttt atcaaaacag
      241 gtaaaaaagc caaaggtagc ttaggtcaag tattcaatca tagcctgtac ggtaagcgtg
      301 aagaaaaata taatgcgtta aatgatgggg aaatatctag cttaccgtgg cagaaactag
      361 atgtaatagc acctgattac ttcttctcgg ttaagaactt tgacaacacc gatttatata
      421 aaaatggttt tagtgttatt gagttgttta gtttgatagg tagtggaata tcttttagga
      481 aagatagttt actaatcaga cagaatgaca ctaaaaaaga cgtatatcaa atgatagatg
      541 atattaaaag cttagacccc agcatattag agtctaaata ttcattcaag cagagtattg
      601 attggaaagt aatagagcaa cgtcaaaact ttttaaatcc tttaaatgat gatattaagc
      661 aggtctcata cagacctttt gatagtaaat atgcttatta ccccctagag aaaatttctc
      721 aaataatccc tagaggggac tctagaaaaa acattatgca gcacatgctt aaagaaaatc
      781 ttactttaat tttcgaacgc agtgtaacta ttggtgattt caccaatata tttataagca
      841 ataaattagt ggaagcgcat gtattaggaa ccatgttttc aaaaggatat gtagcaccac
      901 tttacatcta tcccgatgca tctgatttgc tcgcggaggt atcgcgcata cctaacttga
      961 atatgcagat tgtagaaaca atatcaaaag gtttaaattt aacgtttacc agagagaaag
     1021 aagacctaac caacacaatt gcgccaattg atttacttga ctatatttat gcagtgctac
     1081 atagcccaac ataccgcgag acctataaag agttcctcaa aatcgactat ccacgcgtgc
     1141 cttacccaag tgatgaagaa caattcttcc aactggtagc gctaggtggt gagttgcgac
     1201 aattacactt gcttgagggt gctgcaatca atcaacttat tactagctac cctgaaagcg
     1261 gtaataactt agttgataaa cctcgttata agaacagcaa agtgtatatc aatgacactc
     1321 aatactttga tggcgtacca aagctagcgt gggagtttta tatcggcggc tatcagcctg
     1381 cacaaaaatg gcttaaagac cgtaaaggtc gcatactgag ctatgaggat attttgcact
     1441 atcaaaaaat cattaaagct atggttgaga ctgacaggct aatgcatgaa atagataaat
     1501 ttaaaattgt atgaagtttt agttaatttt ttcgaggtta agatgcgatt taacggccac
     1561 gtaaataggg gtagccatat agtggtgacg gtatttatga cggtattttt ttaaacttat
     1621 aattaagtcc tcatgtttat tggattgcag agcatgttcg aatgacaggg ggccaccaat
     1681 ataaaaaagg cttacatcgc tgcaagcctt tttcttttca ataaattttt tacgtcaacc
     1741 ttgaagccgc gttagtaagt agattaaagg gctgtttgta atttaccgcc cagtcattgg
     1801 atcctgatat agaatcaagc ttcttcctta attcaagtgt agatgataag aaacagtatg
     1861 aagtggctta aattcacggg gattattttc ttgctgttgc tagcagtttt agccgctctg
     1921 ccatttttta tttctttgaa tgattatatt ccactgattg agaataaact ttctgcaaag
     1981 attgatgagc cagttacaat caataatatt caacttagat tgctgccaac gccacatatc
     2041 aatgtaaatg acgtaatggt aggcgttgaa ggtgatttaa aactggctag ggtggtgttg
     2101 acaccagata ttttttcaat atttaaatca tcctatctaa tcaaagatat cgagattgat
     2161 gcgcttgtta ttaatcagaa agcaattaat cggctgatga aatttactaa ggaagcgtca
     2221 tctcctccac atgcaaagcc tcaatttaga gtaggtagac ttagttttac gaatgctcag
     2281 ttaaatttag gtaaattgca ttttgggccg tttcatgcaa atgttaattt agacagtaat
     2341 ggtaagttgt tggaggcctc tttagcaaca gtggacagta aatttcgcgc attgatcaaa
     2401 cctgaaaact tgaattattc tatagtggca agtgctaaaa attggacctt gccgtatgaa
     2461 ccaagattta acctagatga attaagtcta ttggcggtag cgacaacaaa tgatattcaa
     2521 tttagcaaag tgaatgctaa gctttatggg ggtacagcga aaggccaaat ggctataaat
     2581 tggcgaaagg ggctgaaagt gaatggcagc cttgatgtaa ggcaagttga gttggaaaaa
     2641 atcgctaaaa tattatcctc taagacgcat gtaagtgggc aagtcaccgc gaaacctgtt
     2701 ttctctgcat ctgcaaattc gatagaaaat ttgatgcaat caatgcgctt agaagcctct
     2761 tttgatgttc aaaaaggtgt gctgtacggt gttgatatac agtccgcagc aacaaatgtt
     2821 atcaagcaag ggacgactgg cggtcaaaca agttttgagc atctgtctgg ttattttgtg
     2881 atggcgaaaa gcagttatca gttttcaaaa ttaaaaatta catctggaac attgtctgtg
     2941 gaaggcaatg tcaatatatc tcataaaaaa gaattatctg gtcgaattaa cacacaagtt
     3001 aaagtagctg gcattagcac aatagtccct ttgaatgttg cgggcacatt agatgcacct
     3061 aggctacacc ctacagctgc aacaatggca ggggcagcac tgggcactgc ggtgatgggg
     3121 cctggcgtag gaacatctgt gggcgctaag gtgggaggct gggtggatga tttgtttggc
     3181 aaaaagaaaa aataagcaga aaggtaaaca atcagccaat gttgttaaat tggcgtataa
     3241 agtaatcatt caggtcgtaa ataattacga tatgcgatag aggaaaacta aacatgggtg
     3301 aaatagtacc taccgaacga gatgtaccac aagacgatga ttggggaact attggccatg
     3361 tgcaaacgct tgttctcatg atggtaaccg tgcttggtat ttatttgtgt tacaaaatgg
     3421 cgttgccgtt tttggctgtc gttgcttggg ctttgacact agccgtttta tttacaccgc
     3481 tacaaaactg gcttgaagct aaattaaagc gcactggttt ggcagcattc atttcaattt
     3541 tattgattgg ggtgattgtt gtcattccta cattatttgt agggcagcaa ctcgtgattc
     3601 aggcagttaa aggttcgcag atagtcgagc aaaaaataaa ctccggagag tggcggcgtt
     3661 cgctagaggc gcagcctcaa ctggcgccta ttattgatag agttgagaaa tatgtcgatt
     3721 tacccggcac gcttaaaatt tttacaacgt ggctgggtag cgctgcagga gccatcgtta
     3781 aaggttcagt gtatcaagta ttaggaatct gtttgatttt ttacatgctg ttttttttca
     3841 ttcgtgatcg tcgcttagcg cttgccgcca tcaaagcgct atcgccactt tcagaaaatg
     3901 agatggacac catgatacgg cgcattggcg acaccattca tgcaaccgtt tatggcacct
     3961 ttgccattgc agcagtgcaa ggactattag gcgggctaat gttttggtgg cttgacttac
     4021 ctgcgccatt tttatggggt ttggtgatga gtttgctggc tattgtgcca atgttgggag
     4081 cctccattat ttgggcacct gcggcgctat ttttggcatt agatggcaat acgagcagtg
     4141 cattgatgct gacgatttgg ggtgtattcg ttgtgagtac gattgataac ttgctcaggc
     4201 cgatatttgt tggaaacagg ttaaaacttc ataccgtttt agcttttatg tccgttgttg
     4261 gtggactgtt ggtttttggc gcagcaggcc ttattttggg gccagtcacg ttgacaataa
     4321 ccatcacttt gttagatatt tgggccaaga gaacggccgt aaagacgact gatgcataac
     4381 gttcaacaag aaaatgattg ttgcttgtcg ctggaaatgt ttaacggcga cctgccgcag
     4441 cgtcatgcaa atgcgcacaa aggcactttt ggcagtgttg ctgtaatcgg tggagctacc
     4501 ggcatggtcg ggtcggtttt gttggccgcg cgcgcggcat tgcacagcgg agctggccgc
     4561 atatatgcgg cactgttaag tgaaaacgcc cccgcagttg attttataca gccagaaatc
     4621 atgctacgcg ctttagacga cattgtgcaa ttaccacaat taaattgcct agtgattggc
     4681 cccggtttag gtcagtcaat ggtggcgatt aagcaacttg aatattgggt gaatcaaagt
     4741 gttcaaattt tgcttgatgc agacgcgctc aatcttattg ctagtcatcc acatttagcg
     4801 agcgcagtag ctagccgcac ttcaccaaca gtgatgaccc cacatgcggg tgaagcaggg
     4861 cgtttgctgg ctttcagcac tgaagcggtg acgcaaaatc ggctagaaag cgcaataata
     4921 cttgcagaaa agtttaacgc gatatgtgta cttaaaggcg cgggaacgct cattgtgcaa
     4981 gacaaacatc attactttat caacaccact ggtaacgtcg ggctggcaag tggcggcaca
     5041 ggagacgtat taagtggcat cattggcagt ttaatggcgc agggcctcgc ggcaatcaat
     5101 gctgcaaaac ttggtgtttt tgtgcatggt gcggcggctg atgcgctggt aaaaaaaggg
     5161 attggcccta ttgggttaac agcctcagaa gttattttgg aagtgcgtca acagctaaat
     5221 caattgcaca aaaacacagc ataacttttt agatgctctg gaagccttta tttacaaggc
     5281 tcacagagca tttatttttt cacgtagatt ttcttagttg atttgaataa aaatagctag
     5341 gttaattcta cagtttcaag atactcagcg acttcaacat tccagcgtaa ttgctcttca
     5401 atatgcaagc gcatggcatc ggccgcaacg ggttcgccgt ggactaaaaa ggtctttttc
     5461 ggtgggtttt caaaaccgtg caaccaagca atgatttctg aataatctgc atgggcggat
     5521 aagttagaaa catattccac ttgtgcgcgt actggtacgt attcgccatg aattttaata
     5581 ctttcagcgc catcaagcat ggccgcgccg cgtgtgccgg ctgcctgaaa accaacaaaa
     5641 agaacggtgt ttttagggtc tggcgcgaac gcttttaaat gatgtactac gcgcccgcca
     5701 gaggccatgc cacttgcaga taagattatc attggccctt ttgaagtgtt gagtcgttta
     5761 gattcatcta cactattaac aatgtgggcg gtgtgacaga gcgcctggct ttgctcatgc
     5821 gttaagcgat gttcatcttt aaagtggtta aatatggaca tcgcttaacg catgagcaaa
     5881 gccaggcgct ctgtcacacc gcccacattg t
//
LOCUS       DAOQSI010000126         5854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_13821_length_5854_cov_4.135541, whole genome shotgun sequence.
ACCESSION   DAOQSI010000126 DAOQSI010000000
VERSION     DAOQSI010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 5854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5854
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13821_length_5854_cov_4.135541"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PKL42_08060"
     CDS             complement(<1..192)
                     /locus_tag="PKL42_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67067.1"
                     /translation="MNRLKLHNGVTQIEVESKPFSVLRVEQWHDGLLEEKQDCLAEET
                     PVAIVYNGVSHAVMLATAED"
     gene            complement(170..3076)
                     /gene="fdhF"
                     /locus_tag="PKL42_08065"
     CDS             complement(170..3076)
                     /gene="fdhF"
                     /locus_tag="PKL42_08065"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008106461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HNU67068.1"
                     /translation="MDDIKYDKNIDYGTPTSKSTKQVTLTVDDRQVTVPDGVSIMHAA
                     ALAGVMVPKLCATDSLEPFGSCRLCLVEIEGRRGYPASCTTPVAEGLKVHTQTPKLAD
                     IRRGTMELYISDHPLDCLTCATNGDCELQDMAGAVGLREVRYGYEGENHLSAIKDESN
                     PYFTFDPSKCIVCSRCVRACEETQGTFALTISGRGFDSKVSAGITDFMDSECVSCGAC
                     VQACPTATLIEKTVIAAGTPEHSVTTTCAYCGVGCSFDAEMKGEEVVRMTPSKHGGAN
                     HGHSCVKGRFAWGYATHKDRITTPMIRKNIKDPWQQVSWDEAISYAASELIRINQQYG
                     KDAIGGITSSRCTNEEVYVTQKLIRAVFGVNNVDTCARVCHSPTGYGLKQTLGESAGT
                     QTFDSVMESDVIFVMGANPTDGHPVFASQMKRRLREGAKLIIADPREIDLVANSPHIR
                     ADYHLKLRPGTNVALISALSHVIVTEGLVKEDFVKARCEWDSFVEWRDFVAKPENSPE
                     SLANDLGINPELIRAAARLYATGGNAAIYYGLGVTEHSQGSTMVMGIANLAMATGNVG
                     REGVGVNPLRGQNNVQGSCDMGSMPHEFPGYRHVSDDATRALFEAAWGKPLSKEPGLR
                     IPNMLDQASEGSFKALYCVGEDIVQSDPNTQHVTHALEQMECVIVQDLFLNETAMFAH
                     VFFPGASFLEKNGTFTNAERRISPVRRVMAPKNSGLDANGKKEDGMEDWQVTVRLSAA
                     LGYPMEYNHASEIMDEIAALTPTFKGVSFKKLDELGSIQWPCDDENPAGTPIMHIDEF
                     VRGKGKFFITQYVPTTEKVNAKFPLILTTGRILSQYNVGAQTRRTQNTAWHPEDTLEI
                     HPHDAEERGIKEGDWVGIASRAGETVLRAKITPRVQVGVVYTTFHHPESGANVITTEN
                     SDWATNCPEYKVTAVQVSRVSQLSDWQKHYKTFSEAQIAYAQRATRANESTKVA"
     gene            complement(3086..4639)
                     /locus_tag="PKL42_08070"
     CDS             complement(3086..4639)
                     /locus_tag="PKL42_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNU67069.1"
                     /translation="MAIKVYVPCDSTALSLGANKTAEAIVAEAKKRGLEIELVRNGSR
                     GLFWLEPMVEVATNKGRVAYAPVFPKDVPSLFEANFLTGGAHALNLGLTEELAWLKKQ
                     QRLTFARVGITNPISLDDYLAHDGYKGLKNALQMTGAEVVKTVTDSGLRGRGGAAFPT
                     GIKWNTVLGCQADQKYIVCNADEGDSGTYSDRMVMEDDPYVLIEGMTIAGIAVGATQG
                     YIYLRSEYPHALKALNEAISRAEKAGYLGANICGSQHTFNLEVRRAAGAYVCGEETSL
                     LESLEGKRGLVRFKPPLPAIEGLFGKPTVVNNVISLATVPIILDKGAQYYADYGMGRS
                     RGTLPIQLAGNLKQTGLVELAFGATLRELLYDFGGGSASGRPIRAVQVGGPLGAYLPE
                     SQFDTPLDYEEFTKIWAVLGHGGIVAFDDTVDMAKMARYAFEFCAIESCGKCTPCRIG
                     STRGVEVIDKITSGKDHAKNITLVRDLSDTMLNGSLCALGGMTPYPVLSALNHFGEDF
                     GLPANIKAG"
     gene            complement(4639..5118)
                     /locus_tag="PKL42_08075"
     CDS             complement(4639..5118)
                     /locus_tag="PKL42_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HNU67070.1"
                     /translation="MTAVLSTEEITRINAHIDRYQHVQGSLLPLLHAIQDDIGYVPES
                     AYLPISKALALSVAEVHGVVTFYHHFRRHPAGKHILQVCRAESCQAMGSEKLEAHIKS
                     TLGIGYHQTTSDGAITLEPVYCLGNCACSPAVMMDDEVHGRMSAEKIAELIKQARSA"
     gene            5240..>5854
                     /locus_tag="PKL42_08080"
     CDS             5240..>5854
                     /locus_tag="PKL42_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNU67071.1"
                     /translation="MDIKLNTPRLIDQFGRTVDYIRLSITDRCDFRCTYCMAEDMTFL
                     PRNEVLSLEECLRIVKVFVRLGVTKVRITGGEPLVRKNALWLFDEIGKLEHLNELVLT
                     TNGSQLEKHAAALKKSGVKRINISLDSLKPEVFKKITRTGNLKQVLAGLQASIKAGFS
                     GIKLNTVLMRGINDDETADLVQFAINNKIDISFIEEMPLGKVNHA"
BASE COUNT         1569 a         1507 c         1184 g         1594 t
ORIGIN      
        1 atcttcagcg gttgccagca taaccgcatg cgaaactcca ttataaacaa tcgcaaccgg
       61 cgtttcttca gctaaacaat cctgtttttc ttctaaaaga ccgtcatgcc actgttcgac
      121 ccgtagcacg ctaaatggct tactttcaac ttctatctgc gtgactccat tatgcaactt
      181 tagtcgattc attcgcacgt gttgcccttt gagcataagc aatttgtgct tcactaaagg
      241 tcttgtaatg cttttgccaa tccgataact gacttacgcg agatacctgt acagccgtta
      301 ccttatactc tgggcaattg gtggcccaat cagaattttc cgttgtaatc acattggcgc
      361 cagattctgg gtggtggaat gtcgtataaa ccacgcccac ctgaacacgc ggcgtaattt
      421 tggcgcgtaa aacagtttca cccgcacggc tcgcaatgcc cacccaatcc ccttctttaa
      481 tgccgcgttc ttctgcatca tgtgggtgaa tttccaatgt atcttctgga tgccacgcag
      541 tattttgtgt acggcgcgtt tgtgcgccaa cgttgtactg cgacaaaatg cgccctgttg
      601 ttaaaatcaa cgggaactta gcgttgactt tttcagtggt aggcacatat tgcgtaataa
      661 aaaacttacc cttaccacga acaaattcat caatgtgcat aatgggcgta cctgccgggt
      721 tttcatcatc acatggccat tgaatactgc ccaattcatc cagctttttg aagctcaccc
      781 ctttaaatgt aggcgttaac gctgcaattt catccataat ttctgaggcg tggttgtatt
      841 ccatcggata acctaaagct gctgatagcc ttacagtgac ttgccagtct tccatgccgt
      901 cctctttttt accgttagcg tctaaaccgc tatttttagg cgccatcacc cggcgcaccg
      961 gtgaaatgcg gcgttcagca ttggtaaatg taccgttttt ctctaagaaa gatgccccgg
     1021 gaaaaaacac atgcgcaaac atggcggttt cattcaaaaa gaggtcctgc acaatcacac
     1081 attccatttg ctctaatgcg tgggttacgt gttgcgtatt ggggtctgac tgcacaatat
     1141 cttcacccac gcagtacaat gctttaaaac tgccttcact ggcttggtca agcatgttcg
     1201 ggatacgcaa acctggctct ttactgagcg gcttacccca agctgcctca aataaggcac
     1261 gcgttgcgtc atctgacaca tggcgatatc ctggaaactc atgtggcata gaacccatat
     1321 cacatgaacc ttgtacatta ttttgcccac gcaatgggtt gacaccaacc ccttcgcgcc
     1381 ccacattgcc agtagccatc gccaagttag caatgcccat caccatggtt gaaccttgac
     1441 tatgctctgt tacacctaag ccgtaataaa tggcggcatt cccacccgtc gcataaagcc
     1501 ttgctgcggc acgaataagt tcaggattaa tccccaaatc atttgctagg ctttcgggag
     1561 aattttctgg tttagccacg aaatcacgcc attcaacgaa cgaatcccat tcacagcgcg
     1621 ctttcacaaa atcctctttg accaagcctt ctgtcacaat cacatgtgaa agtgctgaaa
     1681 tgagcgccac attggttccg ggccttaatt taagatgata atcagcgcga atatgcgggg
     1741 aattagccac caaatcaatc tcacgcggat ctgcaataat cagttttgcc ccttcacgca
     1801 aacggcgctt catttgtgaa gcaaatacag ggtgaccatc cgtggggtta gcacccatca
     1861 caaaaataac atccgactcc atcaccgagt caaaggtttg tgttccagca gattcaccca
     1921 acgtttgttt taagccatag cccgttgggc tgtgacaaac acgcgcgcag gtgtccacat
     1981 tattcacacc aaacacggcg cgaattaatt tttgcgtgac atagacttct tcgttggtgc
     2041 aacgtgaaga agtaataccg ccaatggcat ctttgccata ttgttgatta atccgaatta
     2101 gctcactggc cgcatagctt attgcctcat cccaacttac ctgttgccaa gggtctttaa
     2161 tgtttttgcg aatcattggc gtggtgatac ggtctttatg tgtcgcataa ccccatgcaa
     2221 aacggccttt cacacatgaa tgcccatggt tggcaccccc atgtttactt ggcgtcatcc
     2281 gcaccacttc ctcgcctttc atttcagcat caaagctaca acctacgcca cagtatgcgc
     2341 aagtggtagt cacggaatgt tctggcgtac ctgccgcaat gactgttttt tcaatcaagg
     2401 tagccgttgg gcaagcctgc acacaggcac cgcaagatac gcactctgaa tccataaagt
     2461 ctgtgatccc agcagaaact ttactgtcaa agccacggcc agagatagta agtgcaaacg
     2521 taccttgcgt ttcttcacac gcacgcacac agcgcgaaca cacaatacat ttgcttgggt
     2581 caaaagtgaa gtaaggatta ctttcgtctt ttatagcact tagatgattt tcaccctcat
     2641 agccataacg cacttcacgc aaacccacag cacctgccat atcttgcaat tcgcaatcgc
     2701 cattggtcgc gcaagtgagg caatcaagtg gatggtctga aatgtacaat tccattgtgc
     2761 cacgacgtat atccgcaagt tttggtgttt gcgtgtggac tttcaagcct tctgccactg
     2821 gggtggtaca tgaggcaggg taaccacgac gaccttcaat ttcaaccaaa caaagtctgc
     2881 atgagccaaa tggctcaagg ctatccgtag cacaaagctt aggcaccatc acaccagcta
     2941 atgcagcagc atgcataatc gatacaccat caggcacggt tacttgccta tcatctactg
     3001 tcaaagtaac ctgttttgtt gatttgcttg tcggcgtgcc gtaatcaatg tttttgtcgt
     3061 acttaatatc gtccataatt gactcctatc ctgcttttat atttgcaggc aaaccaaaat
     3121 cttcaccaaa gtggtttaat gcactcagta ctggataagg tgtcatgcca cccaaagcac
     3181 ataatgagcc attcagcata gtgtcgctta aatcccgcac tagcgtgatg ttttttgcgt
     3241 gatctttacc tgaggtgatt ttatctatca cctcaacccc acgtgtagag ccaatgcggc
     3301 aaggcgtgca tttgccgcaa ctttctatcg cgcaaaactc aaaagcataa cgcgccattt
     3361 ttgccatatc tacagtatcg tcaaacgcca caataccgcc atgtccaagt accgcccaaa
     3421 tttttgtaaa ttcttcgtaa tcgagcggtg tatcaaattg gctttcaggt aaatatgctc
     3481 ctaatgggcc gcccacttgc acagctcgaa ttggtctgcc tgatgcagag ccgccgccaa
     3541 aatcatatag caactcacgt aatgttgcac caaaagccaa ctctactaaa cctgtttgtt
     3601 taagattacc tgccaattga attggcaaag tgccgcgcga acgccccata ccgtaatcag
     3661 cataatactg cgcgccttta tctaaaataa tcggcacagt agcgagtgaa atcacattat
     3721 tcaccacggt tggcttgcca aacaaaccct caatggcggg cagtggcggt ttaaagcgta
     3781 ctaaaccgcg cttgccttct aaactttcaa gtaatgatgt ttcttcacca cacacataag
     3841 cacccgctgc gcggcgtact tctaaattaa atgtatgttg gctaccacaa atgtttgcgc
     3901 ccaaataacc agccttttca gcacgtgaaa tggcttcgtt taaagctttt aaggcatgcg
     3961 gatattcact tcgcaaataa atgtagcctt gcgttgcgcc cactgcgatg cccgcaatgg
     4021 tcatgccttc aatcagcacg tacgggtcat cttccatcac catgcggtcc gaataagtgc
     4081 ctgagtcacc ctcatcggca ttgcaaacta tatacttttg atcggcctga cagcctaaga
     4141 ctgtattcca cttaataccc gtaggaaaag ccgcaccgcc tctgccacgt aaaccagaat
     4201 ctgtcacggt tttaacaact tctgcacctg tcatttgtag cgcgttttta aggcctttgt
     4261 agccatcgtg cgctaaataa tcatcaagtg aaatggggtt ggtaatacct acgcgggcaa
     4321 aagtgaggcg ttgctgtttt ttaagccaag caagctcttc tgttaagccc aaattaagcg
     4381 cgtgtgcgcc acctgttaaa aagtttgcct caaacaagct aggcacatct tttggaaaca
     4441 ccggtgcata cgctacacgt cctttgttag tagctacttc taccatcggt tctagccaaa
     4501 ataggccacg tgaaccatta cgaaccagct caatttctaa gccgcgtttt ttggcttcag
     4561 cgacaatggc ttccgcagtt ttattagccc ctaatgaaag cgcggtagag tcacacggca
     4621 catatacttt aatcgccatt atgcgctcct tgcttgtttg attagttctg ctattttttc
     4681 tgcactcata cgcccgtgca cttcatcatc catcatcacg gctggcgaac aagcgcagtt
     4741 gcctaaacaa tacacaggct ccaatgttat cgccccatct gatgtggttt ggtgataacc
     4801 aatgcccagc gttgatttaa tatgcgcctc taacttttcc gaacccatgg cttggcatga
     4861 ttccgcacga catacctgca aaatatgctt gcccgctggg tggcggcgaa aatggtgata
     4921 gaacgtgaca acgccatgca cttcggccac tgatagcgcc aatgcctttg atatgggcaa
     4981 ataagctgat tctggcacat agccaatatc gtcctgtatg gcgtgtaaca aaggcaacag
     5041 cgacccttgc acatgttgat aacgatcaat atgcgcgtta attctagtta tttcttctgt
     5101 agataacact gccgtcacaa aaacacccca tctatgtacg atttttcgta tgatatacct
     5161 taattaaccc tagttaaagc tgaataaggc ataataaatc gttttaattc gactgtattt
     5221 ttactgcata attcaaccta tggacataaa acttaatacc ccaagactta tcgaccagtt
     5281 tggacgtaca gtggattaca ttcggctctc cattacagac cgttgcgatt ttcgttgtac
     5341 ctattgcatg gccgaagaca tgacattttt acctcgaaat gaagtgttaa gtctagaaga
     5401 atgcctgcgc attgtgaaag tttttgttcg cctcggtgta acaaaagtgc gcatcacggg
     5461 tggtgaaccg ttggtacgta aaaacgcact atggctcttt gatgaaattg gcaagttaga
     5521 acacttaaac gaattggtac tcacaacgaa cggcagccaa ttagaaaaac acgctgccgc
     5581 cctcaaaaaa tctggtgtca agcgcatcaa catttctctg gatagcctta aaccagaagt
     5641 atttaaaaaa attactcgca ctggaaatct gaagcaagtg ttggccggcc tgcaagcgag
     5701 catcaaagcg ggctttagcg gcattaaatt aaacacggta ctaatgcgcg gtatcaacga
     5761 tgatgaaact gctgatttgg tacagtttgc catcaacaat aaaattgata tcagctttat
     5821 tgaagaaatg ccattaggta aagttaacca tgct
//
LOCUS       DAOQSI010000127         5750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_14236_length_5750_cov_4.692362, whole genome shotgun sequence.
ACCESSION   DAOQSI010000127 DAOQSI010000000
VERSION     DAOQSI010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 5750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5750
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14236_length_5750_cov_4.692362"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..1153
                     /gene="alr"
                     /locus_tag="PKL42_08085"
     CDS             68..1153
                     /gene="alr"
                     /locus_tag="PKL42_08085"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNU67072.1"
                     /translation="MRPILATISQSALQHNLSVVRKFAPHSKVMAALKANGYGHGIER
                     IAHGLSGADGFAVLGMDEAITLREMGFEQPILLLEGVFHADSLAVVNAYRLSLVIHCY
                     AQIEMLEHSPYRHPFNIFLKMNTGMNRLGFVPDDYLKALARVTKLTPNVTLMTHFAAA
                     DELNGTDAAMAIFQQTTKNLPYPSSLANSAAILCQPQTHLDWVRPGIMLYGATPAKNT
                     VVTEFGLKPVMQLTSEIIAVQTLNAGETLGYGNRYTASKPTRVGVVACGYADGYPRHA
                     GQYSGSNLLQGVPVAVGGKITQTLGRVSMDMLFVDLTALPEANIGSPVELWGSTVSVD
                     TVAEACGTIGYELLCAVTPRVPFKVVD"
     gene            1153..2526
                     /gene="radA"
                     /locus_tag="PKL42_08090"
     CDS             1153..2526
                     /gene="radA"
                     /locus_tag="PKL42_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNU67073.1"
                     /translation="MAKAKTIYTCTECGASEPKWQGQCPSCMAWNTLVESIDETSAST
                     NRYANKFEGLASTGQLQKISSIKAADIARQPTGISEFDRVLGGGLVEGGVILIGGDPG
                     IGKSTLLLQVLCHLGKVSPAIYVSGEESPQQIAMRAKRLGLDASEVELLAEINLEKIL
                     ATLQTHKPNIAVIDSIQTVYSEALQSAPGSVAQVRECSAQLTRIAKQLGITVILVGHV
                     TKEGSLAGPRVLEHIVDTVLYFEGDQNSTFRLIRAFKNRFGAVNELGVFAMTEHGLRE
                     VSNPSALFLSHHENQVAGSCITVTMEGTRPLLIEIQALVDESHAPNAKRLATGLEQNR
                     LAMLLAVLNRHAGIPCFDQDVFINAVGGVKISEPAVDLAVLLSIVSSLKNKPLENKLI
                     VFGEVGLAGEVRPVQGGLVRLKEAAKLGFTKAIVPKTNVPKQRIEGIEVIGVERLEQA
                     LQQLRNA"
     gene            complement(2600..3385)
                     /locus_tag="PKL42_08095"
     CDS             complement(2600..3385)
                     /locus_tag="PKL42_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNU67074.1"
                     /translation="MGFLAGKKILIAGLLSDRSIAYGIAEAMKREGAELAFTYQVEKH
                     KDRVSKLAADFDSQLVFHCDVAEDSLIDALFVDIAKHWDGIDSIVHSVAFVPKDALDG
                     DYLEKVTRENFRIAHDISSYSFAALAKAAMPLMLGRNGSLLTLSYLGAERTMPNYNVM
                     GVAKASLEANVRYMAQSLGPKGIRVNAVSAGPIKTLAASGIAGFDKMINFNERHAALR
                     RNVTIEEVGNAAAFLCSDLASGITGEITYVDGGMNITAAGVID"
     gene            complement(3446..4267)
                     /locus_tag="PKL42_08100"
     CDS             complement(3446..4267)
                     /locus_tag="PKL42_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNU67075.1"
                     /translation="MKKIFSNPLAIAGFVIIASIMLLALFAPFIAPYNPDAIDVKAIL
                     LGPSNQHLMGTDGLGRDVFSRMLYGARISLLVGFVAVGIATLIGIILGAIAGFYRGWV
                     DTVIMRLVDVMLSIPSFFLILAVIAFLTPSIWNIMIVIGLTSWMGVTRLVRAEFLSLR
                     NREFVLAAQTLGAKDFRLIFTHLLPNSLTPIIVSSILGIGSAVLLESGLSFLGLGVQP
                     PQASWGNILTDGKIYIQFAWWLSLFPGLAILITVLGYNLLGDGLRDAYNPRSNQH"
     gene            complement(4267..5283)
                     /locus_tag="PKL42_08105"
     CDS             complement(4267..5283)
                     /locus_tag="PKL42_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNU67076.1"
                     /translation="MLKYIIKRFLWMIPMLIGISLISFLIMQLAPGDITTTEANFNPK
                     ASEESRQKLRTMYNLDKPVIVQYGLWLKRMATLDFGNSFASHQRPVFWQTKDKDGNIT
                     LGMIQEALPITLLINVLSLALIIMIALPLGVISALKQNQLADRAITIFVFIGFAIPGF
                     WLALMLMYWTGVQHHWLPISGLQSMGSEHLSWFAQTIDFIKHLILPVFISAITGLAGI
                     SLFTRNGMLEVLHQDYITTARSKGLSENRVVYGHALRNALLPLITLLGLSIPGLIGGS
                     VIAESIFAINGMGKLFYDAVLMRDFPVIMGILTIGALLTLIGNLLADVAYAWADPRVR
                     RGVM"
     gene            complement(5294..>5750)
                     /locus_tag="PKL42_08110"
     CDS             complement(5294..>5750)
                     /locus_tag="PKL42_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HNU67077.1"
                     /translation="RRLKDVGIDVKIRILEWASFLGRFIEPREFDAVVLGWSLGLDPD
                     QYNIWHSSQQGPKQFNFVGYNNPKVDALLEAGRLELDPDKRMKIYHEFSKILLEDSPI
                     VYLSAGYGLNAIHKRVQGISNPAPPAGIGHNTHEWYIPSQYARNELSEY"
BASE COUNT         1632 a         1240 c         1343 g         1535 t
ORIGIN      
        1 acatacgcgg tttgaaaatt ttgccggtac aagttatatg gcggatgagt actaaccaga
       61 ctgtactatg cgccccattt tagccactat ttctcaaagt gccttgcaac acaatctgtc
      121 cgttgtaaga aaatttgccc cacattcaaa ggtgatggca gcattaaaag ctaatggtta
      181 tgggcatggc atagagcgta ttgcacatgg tttaagtggt gcagatggct ttgccgtgct
      241 tggaatggac gaggccatta cattgcgtga aatgggtttt gaacagccga ttttgctgct
      301 tgaaggcgtg tttcatgcgg actcacttgc ggtagtgaac gcttatcgct taagtcttgt
      361 gatacattgt tacgcgcaaa ttgagatgct tgagcattcg ccatatcgcc atccattcaa
      421 tatttttctt aaaatgaata cgggaatgaa ccgtttaggt tttgtaccag atgattattt
      481 aaaagcgcta gcgcgtgtta caaaactcac gcccaatgtc acccttatga cgcactttgc
      541 agcggcagat gaactgaatg gcacagatgc tgccatggct atttttcaac aaaccacgaa
      601 gaatttacct tacccttcat cactcgcaaa ttccgcagcc attttatgcc aaccgcaaac
      661 gcatctagat tgggtgcgcc caggcattat gctttatggt gcaacgccgg caaagaatac
      721 agtagtaaca gaattcggtt taaaacctgt gatgcaattg acgagcgaaa ttatcgctgt
      781 ccaaacgtta aatgctggtg aaactttagg ttatggaaat cgctacactg ctagcaagcc
      841 tacacgagtg ggtgttgtgg cgtgcggcta tgcggatggt tacccgcgcc acgcaggcca
      901 atattcaggc agcaatctct tacaaggcgt acctgtggcg gttgggggta aaataacgca
      961 aacccttggg cgtgtttcga tggacatgct ctttgtggat ttaactgcac ttcctgaagc
     1021 gaacataggt tcaccagtcg aactttgggg cagcacagtg agtgttgata ctgtagcaga
     1081 agcgtgcggt accatcggtt atgaactctt atgtgccgtt acgccgcgcg tgccgtttaa
     1141 ggtggtggat taatggctaa agcaaaaacc atttacacct gcaccgaatg tggcgcatcc
     1201 gagccaaaat ggcaagggca atgcccaagt tgcatggctt ggaatacatt agttgaaagc
     1261 attgacgaga cgagtgcttc aacgaaccgc tacgccaata aatttgaagg cttggcaagc
     1321 acaggtcaac tgcaaaaaat tagtagtatt aaagcggctg atattgcgcg ccaaccgaca
     1381 ggcattagtg aatttgatcg tgtactgggc ggtggtttag tggaaggcgg ggtgatttta
     1441 attggcggcg accctggcat tggtaagtcg acgcttcttc tgcaagtgct atgtcatttg
     1501 ggcaaagtca gccccgccat ttatgtgagt ggggaagagt cgcctcagca aatcgcaatg
     1561 cgtgccaagc ggttggggtt agatgccagc gaagttgagc ttttggctga aattaacctt
     1621 gaaaaaatcc ttgccacctt gcaaacgcac aaaccaaata ttgcggtgat tgattcaatt
     1681 caaacagtct attcagaagc gttgcaatcc gcacctggtt cggtagcaca agtgcgcgag
     1741 tgttctgcgc aattgacacg cattgccaag caattaggga ttactgtcat attggttggt
     1801 catgtcacca aagagggttc attagcaggc ccgcgcgtac tagagcatat tgtagatacg
     1861 gtgttgtatt ttgaaggtga ccaaaactca acttttaggc tcattcgcgc atttaaaaat
     1921 cgttttggcg cagtgaatga actgggcgta tttgcgatga cggaacatgg cttacgtgag
     1981 gtgtcaaatc cttcagcttt gtttttgtcg caccatgaaa accaagttgc aggtagctgc
     2041 attaccgtga cgatggaagg cacgcgtccg ctgttaatag aaattcaggc cttagtagat
     2101 gaaagccacg cgcccaatgc taaacgctta gccacagggc ttgagcaaaa ccgtttagcg
     2161 atgttgttgg cggtattaaa tcgccatgca ggcatacctt gttttgacca agatgtgttt
     2221 attaacgcag taggtggggt gaaaattagt gaacctgcag tagatttagc cgtgcttcta
     2281 tctattgttt cttcattaaa aaacaagcca ttagaaaata aacttattgt atttggtgag
     2341 gttggtttgg cgggagaagt gcgccccgta cagggtggat tagtgcgttt aaaagaagcc
     2401 gcaaaattag gctttacgaa agccattgtg ccaaaaacca atgtgccaaa acagcgtatt
     2461 gaggggattg aagtgattgg tgtggagcgt ttggagcaag cgcttcagca gttaagaaat
     2521 gcgtgactgc gtaaactgca aattattcta actaaaaaag ggcgcattca atgcgccctt
     2581 aaatgattaa gtcaatgcgt taatcaataa cgcctgccgc agtgatattc atgccaccat
     2641 caacataagt aatttcacca gtaatacctg aagctaaatc gctacacaaa aacgcagcag
     2701 cattgcctac ttcttcaatc gtaacattac gacgtaatgc cgcatgcctt tcgttgaagt
     2761 taatcatttt atcaaaacca gcaatgccag aagcggctag cgttttgatt gggccagcag
     2821 atacagcatt gacgcgtata cctttaggac ctaaactttg tgccatatag cgtacattgg
     2881 cttcaaggct ggcctttgca acacccatca cgttgtaatt tggcattgtg cgttccgcgc
     2941 ctaaatagct cagtgtcagc aggctgccat tgcgacccag catcagcggc attgcggctt
     3001 ttgccagtgc ggcaaagctg tatgaactga tatcatgtgc gatacgaaag ttttcacggg
     3061 tgactttttc aagataatca ccatctaatg cgtcttttgg cacaaaggcg acagaatgca
     3121 cgatggaatc aatgccatcc caatgttttg caatatcaac aaaaagcgcg tcaattaggc
     3181 tgtcttcagc aacatcgcag tgaaatacaa gttgagaatc aaaatccgcg gccagtttac
     3241 ttacgcggtc tttgtgtttt tctacttgat atgtgaatgc gagttcagca ccttcacgtt
     3301 tcattgcttc agcaatgcca taagcgatgg atcgatcgct taataaacca gcgatgagta
     3361 tttttttacc tgctaagaat cccataagat tgtccctatc aatatttaat atttattttt
     3421 gtcttaatca atgcaatatt aaaatttagt gttgattgct tctggggttg tacgcatcgc
     3481 gtaaaccgtc acccaacaga ttatacccta acaccgtaat caaaatagca agacctggga
     3541 aaagagacag ccaccaagca aactgaatgt atatttttcc atcagtcaaa atattgcccc
     3601 atgaagcctg tggaggttgc acgccaaggc ctaaaaagct taagcccgat tctaataaaa
     3661 ctgcgctacc aattcctaat atggaactga cgataattgg cgttagactg ttaggcaata
     3721 gatgcgtgaa aatcagccga aaatcttttg cgcctaatgt ttgtgctgcc agaacaaact
     3781 cacggttacg taaacttaaa aattctgcgc gcactaagcg tgtgacgccc atccatgagg
     3841 ttaaaccaat cacaatcata atattccaga tggaaggtgt tagaaaagca atgaccgcta
     3901 aaattaggaa aaaacttgga atggaaagca tcacatcaac taaacgcatg atgacagtat
     3961 ccacccagcc tcgatagaaa cctgcaatcg cgccaagaat aatgccaatc aatgttgcaa
     4021 tgcccaccgc aacaaagcca acaagtagtg atatgcgcgc accgtatagc atccgcgaga
     4081 atacatcgcg accaagtcca tcggtcccca ttaaatgctg gttagatggg ccgagtagaa
     4141 tggcttttac atcaatcgca tccggattat aaggggcgat gaagggcgcg aagagggcta
     4201 atagcataat gctggcaata atgacaaaac cagcaatggc caatgggttg gaaaatattt
     4261 ttttcattac attactccgc gtctcactct tgggtctgcc cacgcgtaag caacgtctgc
     4321 taacaagttg ccgatgagtg taagtaacgc gcctatggtc agaatgccca taattactgg
     4381 aaaatcgcgc attaacacag catcgtaaaa gagtttgccc ataccgttaa tggcaaaaat
     4441 gctttcagcg atgacagagc cgccaattaa gccgggaata cttaagccta gtaatgtgat
     4501 gaggggtaaa agcgcattgc gaagcgcatg accataaacg acgcgatttt cgcttaaccc
     4561 ttttgagcgg gcagtggtaa tgtagtcttg atgcaggact tcaagcatgc cgtttcgtgt
     4621 aaatagtgat atgcctgcta aaccagtaat tgccgagata aatacgggaa ggatgagatg
     4681 tttaatgaag tcgatggttt gtgcaaacca ggaaagatgc tcactcccca tactttgtag
     4741 tccagaaatg ggtagccagt gatgttgcac gcccgtccaa tacatcagca ttagggcgag
     4801 ccaaaaaccg gggatagcaa atccaataaa cacaaatata gtaatggcgc ggtcagcaag
     4861 ttgattttgc tttagcgctg aaattacacc cagtggcaaa gcaatcataa tgatcagcgc
     4921 taggcttaac acattgatga gtagcgtgat gggtagcgct tcttgaatca tgcctaatgt
     4981 gatatttccg tctttatctt tagtttgcca aaagactggg cgctggtgcg atgcaaatga
     5041 attgccaaaa tcgagcgttg ccatccgttt tagccacaaa ccatactgca caataacagg
     5101 cttgtctaag ttatacatgg tgcgtagttt ttgccgtgat tcctcactgg ccttggggtt
     5161 gaagtttgct tcggtcgtgg tgatgtcacc tggggctaat tgcataatca gaaaggagat
     5221 taaactaata ccaatgagca ttggtatcat ccataaaaat cgcttaatga tatatttcag
     5281 catgacagtt ttcctaatat tcgcttaatt cgttgcgcgc atattggctt ggaatatacc
     5341 actcgtgggt attgtgccca ataccggctg gtggtgcagg attgctgatg ccctgcacgc
     5401 gcttgtggat tgcatttaag ccatatcctg cggacaaata gacgatgggg ctgtcttcca
     5461 gtaatatttt tgaaaactcg tggtaaattt tcatacgttt gtccgggtca agttcaagcc
     5521 tgccagcttc taaaagtgca tctacttttg ggttgttgta accaacaaaa ttaaattgct
     5581 ttgggccttg ctggcttgag tgccaaatgt tgtattggtc tgggtctagc cctaagctcc
     5641 agccaaggac gactgcatca aattctcgcg gttcaataaa ccgacctaag aagctggccc
     5701 attctaaaat tctaattttc acatcaatgc caacatcttt tagccgcctc
//
LOCUS       DAOQSI010000128         5686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_14466_length_5686_cov_5.062689, whole genome shotgun sequence.
ACCESSION   DAOQSI010000128 DAOQSI010000000
VERSION     DAOQSI010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14466_length_5686_cov_5.062689"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..368)
                     /locus_tag="PKL42_08115"
     CDS             complement(27..368)
                     /locus_tag="PKL42_08115"
                     /inference="COORDINATES: protein motif:HMM:NF023014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3240 family protein"
                     /protein_id="HNU67078.1"
                     /translation="MSETEKKLIDSDNPSEQNPCLVMLFATPALEESLVDWLLENESI
                     YGFSTTESYGHGQRASAMSLLEQVTGRQRRIQFLIEINQNLAPVLLQSLKLSFPGLHF
                     LVIPVLSAGSL"
     gene            complement(383..3448)
                     /locus_tag="PKL42_08120"
     CDS             complement(383..3448)
                     /locus_tag="PKL42_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNU67079.1"
                     /translation="MLNKLIQFSLTQRLLVMLCTVLLIAGGAYAFKELPIDAFPDVST
                     TQVKIIIKAPGMTPEEVESRITAPIEVEMLGIPKQTMLRSVAKYALTDITVDFADGTD
                     IYWARQQVAERLSAIWSNLPTDINGGIAPMTTPLGEMFMFTVEGDDLTIEQRRGLLDW
                     VIRPALRTVNGVADVNALGGVVRTFEVVPNNAKLYARGITLADLQSTLELNNRNDGAG
                     RLSDGEESLLVRAEGKFQTIDDIQNTIVRASQGMPVRISDVAEVKMGALTRYGAVTKN
                     GKSEAVEGLVLGLRGANAQQVVKNVRAKLDEIQPTLPKGVSVDVFYDRGNLVERAVNT
                     VTRALLEATILVLVLLVLFLGNFRAAVTVALVLPLAALVTFILMAQFGMSANLMSLGG
                     LSIAIGMLVDGAVVVVENIVSHLAHGQNGKLPRLHLIYRAVREVSIPVTSGILIIITV
                     FLPLLTLQGLEGKLFSPVALAVVFALAGSLVLSLTVIPVLASFLLKKVSHEEPWVVRQ
                     AIKLYEPVLRWSLANTRVISAIVVATLAVTVIVYLQIGKTFMPVMDEGDILMQIEKLP
                     SINLEKSLELDLAVQRKLMEKVPEVKGVVARVGSDELGLDPMGLNQTDSFLVLKPKNE
                     WRMRDKEALTDEIRKVLDDMPGISYAFTQPIEMRVNEMILGVRGDLAIKIFGNDLVKL
                     DEMAQQTVKILKEIPGSQDVYTPQNSGVQYMQVTIDRFAAGRLGFNVSEVESALRTQL
                     EGRQIGIVQEGLRRTPIVLRGSESLRASPAEFANLMLTLPNGNNVPITTVAKLERIEG
                     PVKVDREKGARMVVVVANVRGRDLVGFVEEAKNKVQQQIKLPEAYSMQWGGQFENQQR
                     AAARLAVVVPVALGLIFLLLFATFRSIRQSLLILSNIPFAMVGGIFALWFSGEYLSVP
                     ASVGFIALLGIAVLNGVVMVSYFNQLLAHGMALGEVVVEGAKRRLRPVLMTANIAAFG
                     LIPLLFATGPGSEIQRPLAIVVIGGLISATSLTLILLPILYQKFGVETK"
     gene            complement(3482..4579)
                     /locus_tag="PKL42_08125"
     CDS             complement(3482..4579)
                     /locus_tag="PKL42_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNU67080.1"
                     /translation="MIKIKSVCLFVFMLGQLSVSHAESLIAMTPQQMQTLGIAVSPIV
                     ATNAVVSNKLPGEIIIPVNQERIVSTPQSGLIDAMYFAAGQSVKRGQVVAHISSTELV
                     ALQGDYLRGKTQHQLSKNMADRDRELYKDGIIAQRRLLTTESSHQELSVELKQRRQAL
                     KMAGMGENAIAKLEKSGELASGFTLTSPIDGVVIEQLGSTGQRVDASMPIYRIAKLKP
                     LWLEIHAPIEIVHFAKEGMMVNIPKYQASGKITTIIRNLNRNDQTVHLRAEVTVGTEK
                     LFPGQFVEAELVSESTTRQFAVPKSAVIRNGNKSYVFVQNTKGFVPKQVVVSSEQSTQ
                     AVINGDFTGKESVAISGTVAIKSAWYSTTTQ"
     gene            complement(4576..>5686)
                     /locus_tag="PKL42_08130"
     CDS             complement(4576..>5686)
                     /locus_tag="PKL42_08130"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNU67081.1"
                     /translation="SILRSPKLAEIQAGKGLVNAKNIQASSFLPGAPAVTAGHQNDVV
                     GSNRNLNQWEVGLDLPLWLPGQKSARASLAEGSKTELDKTSANLSLDIAGQVRDALWN
                     FGMATNEAELQESRYLAAQALLTDVEKRWKAGELAKTDVMLAQNGTLAAKTALIKAKA
                     EVKHAEHRYWILTGLKELPNTFEEQLTSKEMIDDSHPWLAESAAKIEIANHQRALNAI
                     EQRENPQVMLNVRHERGAFDSLYNDSVGVAIRVPLDSKVRSAPMLANAEMAIAQAMSE
                     HEQRQRLLESAFHEAQHNLEVIREEIDILQAQHKLSQESLQLAKKSFALGESDLIDLL
                     RIQATAYEAERALKSRQTQMQWHIARYNQAIGVLP"
BASE COUNT         1553 a         1453 c         1105 g         1575 t
ORIGIN      
        1 cattaattcg acaatacttc taaatatcag agacttccag cacttaaaac gggtatgact
       61 aaaaaatgta accccggaaa actcaactta agagattgca gtaatacagg tgctaaattt
      121 tgatttatct caatcaaaaa ctgaatgcgg cgttgacgac cggtgacttg ctcaagcaat
      181 gacattgccg atgcacgttg cccatggccg tagctttctg ttgtactaaa cccataaata
      241 ctttcgtttt caagtagcca atcaacaagg ctctcctcta gtgcaggtgt tgcaaatagc
      301 atcacaaggc atgggttctg ctcagagggg ttgtcactat caatcagttt tttttcagtt
      361 tcactcattt tgtttgtagc ctttatttgg tttctacacc aaatttttga tataaaattg
      421 gtagtaatat cagcgttaag ctagttgcac tgattaagcc gccaatcaca acaatggcta
      481 atgggcgttg aatttcagaa ccaggccctg tagcaaatag caaggggatc aagccaaaag
      541 ccgcaatatt cgctgtcatc aatactgggc gtagcctgcg tttggcccct tccactacca
      601 cctcgccaag tgccatacca tgcgctagca attggttaaa atacgaaacc atcaccacgc
      661 cattcaacac cgctatccca agtagcgcaa taaaaccgac cgatgcaggg acagataaat
      721 actcgcccga aaaccataac gcaaaaatac cgccaaccat cgcaaagggg atgtttgaaa
      781 gaatgagtaa agattgccta attgaacgaa atgtcgcgaa aagtagcaaa aatatcaacc
      841 ccagcgcaac cggaaccacg accgccaacc ttgcggcagc tcgttgctga ttttcaaact
      901 gaccacccca ctgcatagag taagcttcag gcagtttaat ttgttgctgg accttatttt
      961 tagcctcctc gacaaaccca actaaatctc gccctcgaac attcgccacc acaacgacca
     1021 tgcgcgcacc tttttcacgg tctaccttaa ccggcccttc tatacgctct aatttagcta
     1081 ctgtagtaat gggtacatta ttgccattgg gtaaagttag cattaaattt gcaaactcag
     1141 caggggacgc acgtagcgat tcagaccctc tcaatacaat cggtgtgcgc cttaaacctt
     1201 cttgcacaat tccaatttgg cggccctcca gttgcgttcg taatgcactt tcgacttctg
     1261 aaacattaaa ccccaaacgg ccagcagcaa atcggtctat cgtcacttgc atgtactgta
     1321 cgccgctatt ttgtggcgta tatacatctt gtgaaccagg aatttcctta aggattttga
     1381 cagtttgctg ggccatttca tcaagcttca ctaagtcgtt accaaaaatc ttaatcgcca
     1441 aatcgcctcg cacacctaaa atcatttcat tgacacgcat ttcaatgggt tgcgtaaacg
     1501 catagctgat acctggcatg tcatctaaca ccttacgtat ctcatcggtt aaagcctctt
     1561 tgtcgcgcat gcgccattca ttttttggct taagcacaag aaaactgtcc gtttgattca
     1621 accccatcgg atctaagcct agttcatcag agccaacacg ggctaccaca cctttgactt
     1681 ctggtacttt ttccatgagt ttgcgctgta ccgctaaatc taactccaga cttttttcta
     1741 aattaataga gggtaacttt tcaatctgca tcaagatgtc gccttcatcc atcaccggca
     1801 taaaggtttt accaatctgt aaatacacga ttacagtaac agccaatgta gcgacaacaa
     1861 tcgctgaaat aacgcgcgta ttggctaatg accaacgtaa taccggttca tacaacttaa
     1921 tggcctgccg tacaacccaa ggctcttcat gactaacctt tttcaataaa aaagatgcca
     1981 gcaccggaat aaccgttaaa gacagcacta gcgaacctgc cagcgcaaag acaaccgcga
     2041 gagctacagg gctaaatagc ttgccttcta agccttgcaa tgtaagtagc ggtaaaaata
     2101 ctgtgataat aatgagtatg ccggatgtca ctggaatact cacctctctg acagcccgat
     2161 aaatcaagtg taatcgaggt aactttccat tttgaccgtg cgcaagatgc gagacaatat
     2221 tttcaacaac aacaaccgct ccatccacca gcataccaat ggcgatagac aatccaccca
     2281 aactcattaa gttggctgac atgccaaact gcgccattaa aataaatgtc acaagcgctg
     2341 ccaatggtag cacgagcgcc accgtcacgg ccgccctaaa attacctaaa aacaatacaa
     2401 gcaagaccag cactaaaatg gtcgcttcca acaatgcccg cgtgactgtg ttgaccgcgc
     2461 gctcaactaa attaccgcgg tcgtaaaata catcaacact gacgcctttt ggtaaggttg
     2521 gctgaatttc atcaagcttg gcgcgtacgt ttttaactac ttgttgagcg tttgctcctc
     2581 ttaagcctaa aaccaagccc tcaacagcct cgcttttgcc gtttttagtc acagcgccat
     2641 agcgtgtaag cgcacccatc ttaacttctg caacatcact tatacgcacc ggcatgcctt
     2701 ggctcgcgcg tacaattgtg ttttgaatat catcaatcgt ttgaaattta ccctcagcgc
     2761 gcaccagcaa agattcttcg ccatcactca agcgccctgc gccatcatta cgattattaa
     2821 gttccaaggt actttgcaaa tcagctaaag taattccgcg cgcataaagc ttggcattat
     2881 tcggcaccac ttcaaaagta cgaacaacgc caccgagcgc attcacatct gctacgccat
     2941 taactgttct aagtgctgga cgaatgaccc aatcaagcag gcctcggcgc tgctctattg
     3001 ttaaatcatc cccttctaca gtaaacataa acatttcacc tagcggcgtt gtcattggcg
     3061 ctattccgcc attaatatct gtgggcaaat tgctccaaat cgcgcttaaa cgctccgcaa
     3121 cctgctgcct tgcccagtaa atatccgtac catcggcaaa gtctaccgta atgtctgtta
     3181 aggcatattt agcaactgag cggagcatgg tttgctttgg aatacccagc atttcaactt
     3241 caataggtgc tgttatgcgc gactccactt cctctggcgt catgcccggc gctttaataa
     3301 taatcttaac ctgtgttgta gaaacatcag gaaacgcatc tatcggtaac tccttaaacg
     3361 cgtaagcgcc accagcaata agcaataccg tgcagagcat taccagcaag cgttgcgtaa
     3421 gtgaaaattg aataagttta ttaagcatga ataacccgaa atattgaaat agctgtttag
     3481 cctattgagt ggtggtgcta taccaagcag atttaattgc aacagtaccg ctaatggcaa
     3541 cgctttcttt gccagtaaaa tctccgttaa tcacagcctg tgtggattgt tcgctggaaa
     3601 caaccacctg ttttggaaca aaacctttag tattctgaac aaaaacatag cttttatttc
     3661 catttctaat aacagcagat tttggcactg caaattgacg cgtggtgctt tcacttacca
     3721 attcagcttc aacaaattgg ccaggaaaaa gcttttcagt ccctacggtt acttctgcgc
     3781 gcaagtgcac ggtttggtcg ttccgattca gatttcgaat aatcgtggtg atttttcctg
     3841 aagcttgata cttcggaatg ttgaccatca tgccctcttt tgcaaaatgc actatctcta
     3901 tcggcgcatg aatttcaagc cacaatggct ttaattttgc aatacgataa attggcatgc
     3961 tcgcatctac acgttgcccg gtagacccca attgttcaat cacaacacca tctatggggg
     4021 atgtcagggt aaacccgctc gctaattcgc cacttttttc taactttgca attgcgttct
     4081 cacccatacc agccattttc agcgcttgtc ggcgttgctt taattcaaca gataattctt
     4141 gatggctact ttctgtagtc agcaatcgac gctgagcaat aatgccatcc ttatataact
     4201 cacggtcccg atctgccatg tttttgctca gttggtgctg cgttttgcca cgtaaatagt
     4261 cgccctgcag cgcgactagt tctgtgctgc tgatatgtgc aactacttgg ccgcgcttaa
     4321 ccgattggcc tgcggcaaag tacattgcat caatcaaacc gctttgaggt gtactgacga
     4381 tgcgctcttg attgactgga ataataattt ctcctggcaa cttattgctc acgacagcgt
     4441 ttgtagcaac aatcggtgaa acggcaatgc caagcgtctg catttgctgt ggcgtcattg
     4501 caattaaact ttccgcatga ctcactgata gctggccgag cataaaaacg aacaaacaaa
     4561 cggatttaat ttttatcaag gtaaaactcc aatagcttgg ttataacgtg cgatatgcca
     4621 ttgcatctgg gtttgacggc ttttcagcgc gcgctctgct tcataggcag ttgcctgaat
     4681 gcgtaacaaa tcaatcaagt ctgattcgcc aagtgcgaaa gattttttag ccagttgcaa
     4741 gctttcttga gaaagtttgt gttgtgcctg caaaatatca atctcttcac gaatcacttc
     4801 taagttatgt tgcgcttcat gaaaagcact ctcaagtaag cgttggcgtt gttcatgttc
     4861 actcatcgcc tgtgctatgg ccatctcagc attcgccagc atcggtgctg agcgcacttt
     4921 tgaatctagt ggaacgcgaa tcgctacgcc tacgctatcg ttgtaaaggc tatcaaaagc
     4981 gccacgttca tgacgtacat tcagcatcac ttgtggattt tctcgttgct cgatggcgtt
     5041 aagcgctcgc tgatgattgg caatttcaat cttggccgct gattctgcca accatggatg
     5101 gctatcatca atcatttctt ttgaagttag ctgctcttca aatgtgttag gcagttcttt
     5161 taaccctgtg agtatccagt aacggtgttc cgcatgcttc acttcagctt ttgccttaat
     5221 taaagcggtc ttggcagcca aagtaccatt ctgcgccagc atgacatcgg tcttggcaag
     5281 ctcgcccgct ttccagcgtt tttctacatc tgtgagcaaa gcctgtgctg ccagatagcg
     5341 tgactcttgc agctctgcct catttgtcgc catgccaaaa ttccatagcg catctctcac
     5401 ctgccctgca atatctaacg acaagttggc actggtctta tccagctcag tttttgaacc
     5461 ttctgctaaa gacgcacgcg ctgatttttg accaggtaac caaagtggta aatcaagacc
     5521 cacttcccac tgatttaaat tgcgattgct tccgacaaca tcattttgat gccctgccgt
     5581 aaccgcaggc gcgccaggta aaaaactgct tgcttgtata ttttttgcat taactaagcc
     5641 tttacctgcc tgaatttcag ccagtttagg gcttcgcaaa attgat
//
LOCUS       DAOQSI010000129         5583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_14856_length_5583_cov_5.512663, whole genome shotgun sequence.
ACCESSION   DAOQSI010000129 DAOQSI010000000
VERSION     DAOQSI010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14856_length_5583_cov_5.512663"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="PKL42_08135"
     CDS             complement(<1..865)
                     /locus_tag="PKL42_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HNU67082.1"
                     /translation="MENKPAVSSGKCPVMHGGNTVASNTNTDWWPNALNLDILHQHDS
                     KTNPLGEDFDYAQAFKTLDLAAVKNDLKALMTDSQDWWPADWGHYGGLMIRMAWHAAG
                     TYRIADGRGGAGTGNQRFAPLNSWPDNVNLDKARRLLWPIKKKYGNKLSWADLIVLAG
                     TVAYASMGLKTFGFAGGRADIWHPEKDIYWGAEKEWLGNASRYDGEKTRDSLENPLAA
                     VQMGLIYVNPEGVDGKPDPLKTARDVRLTFARMAMNDEETVALTAGGHTVGKAHGNGD
                     ASKLGPNPEAAD"
     gene            1138..2037
                     /locus_tag="PKL42_08140"
     CDS             1138..2037
                     /locus_tag="PKL42_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNU67083.1"
                     /translation="MTLTELKFIVAVAHERNFRRAAEKCFVTQPALSLAIKKLEEELN
                     VVIFERSRTDVSMTEIGIKIVEQASKILQEATQLKQIAELGKNQLKGPLKLGMIHSVG
                     PYLLPEIIPILRKIAPDMPLEVEENLTANLETQLRGGVIDVAIIALPFDVPGLQYRAL
                     YDEDFDVVVPSNHHWVHRKHIKPEELSNEKVLLLNAGHCFSNQVTQACPELSRKGEVL
                     QGNSLETIRNMVASNLGISVLPCSATAARYHNPLIQVIPFSAPAPTRRIAIAWRKSYM
                     RMEAVEKIVQAIQAVHADFMHLL"
     gene            complement(2339..3385)
                     /locus_tag="PKL42_08145"
     CDS             complement(2339..3385)
                     /locus_tag="PKL42_08145"
                     /inference="COORDINATES: protein motif:HMM:NF023334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon and DUF3426 domain-containing
                     protein"
                     /protein_id="HNU67084.1"
                     /translation="MSNITNCPNCQTQFVVSKKQLKQYQGKVRCGHCLHVFDATMHMV
                     EAIPNEGIADATQEVLSVSTDNFQPTDVSISAVYAVSASFHDHDVDTDTPSDNENAWP
                     TIIDDIEHEVSQATISSPILNNAPAEPTVPDTPDIVDEDNIAEVIDYGALAPSFSEVP
                     LEPEATVETPKPISNLSVDAKFYKKKAPRSKASTWLLFCVTTLLGLAAIGQSVYFLRN
                     EIPIYYPNSKPYLVSACEKIGCEITLPKKIEYIFIDDFGIEEDAEYAGLMRLTSTLIN
                     QASFAQTYPNLELTLKDADDNPKLRRTLKPAEYLPQNTAVENGIGPGEQVKIKLNITT
                     NGEEVSGYSALVTY"
     gene            complement(3394..4281)
                     /gene="prmA"
                     /locus_tag="PKL42_08150"
     CDS             complement(3394..4281)
                     /gene="prmA"
                     /locus_tag="PKL42_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNU67085.1"
                     /translation="MAWISLKIAAQDNTADLISDTLMAQGALSAIIEDANADTLDEQP
                     IFGEPGDPPPGIWQQNLVSALFDDDVDVAAIVADLQRKTKLNYLTYSTEVIEEQDWVR
                     ATQSQFDPIKITDDLWIVPTWHNAPNANALNIVLDPGLAFGTGSHPTTHLCLAWLKKT
                     VQPNNSVLDYGCGSGILAIAAKKLGAAEVVGTDIDPQAIQSSLYNAEQNQVQASFLHA
                     SLYQSKAFDIVVANILSSALSVLAPALANSCKSGGKIALSGILKEQAADVSAIYADWF
                     NMDSPQTMDAWVLLTGTKK"
     gene            complement(4345..>5583)
                     /gene="accC"
                     /locus_tag="PKL42_08155"
     CDS             complement(4345..>5583)
                     /gene="accC"
                     /locus_tag="PKL42_08155"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNU67086.1"
                     /translation="EAKYVKLADESVCIGPAPSSQSYLNIPAVISAAEVTDAQAIHPG
                     YGFLSENADFADRVEQSGFIFIGPRAETIRLMGDKVSAKNAMRKAGVPCVPGSEGALP
                     EDPAEVMKIAKQIGYPVIIKAAGGGGGRGMRVVHTEAALLASVNMTKAEAQAAFGNPV
                     VYMEKYLEKPRHIEIQVLADQHGNAVFLGERDCSMQRRHQKIIEEAPAPLLNPKLRDK
                     IGERCAEACRKIKYRGAGTFEFLYENGEFYFIEMNTRLQVEHTVTEMITGIDLVQQQI
                     FVASGEKLKFRQKDIQLRGHAIECRINAEDPYSFVPSPGTINRFHMPGGPGVRVDTHA
                     YGGYTVPPHYDSMIGKLITYGDTRDQAIARMRIALSEMMITGISSNIALHSDLMADAA
                     FHAGGTSIHYLEQKLGIYNK"
BASE COUNT         1502 a         1313 c         1240 g         1528 t
ORIGIN      
        1 catcggctgc ttctggatta ggcccgagtt tgctcgcatc gccattgcca tgcgctttgc
       61 ctaccgtatg accaccggca gtcaatgcaa cggtttcttc atcattcatg gccatgcgtg
      121 caaaggtgag gcgcacatcg cgagcggttt tgagcgggtc tggtttgcca tccacgcctt
      181 cagggttcac ataaatcagc cccatttgaa cggcagctag cggattttca agtgaatcac
      241 gggttttttc accatcataa cgacttgcat tacctagcca ttctttttca gcaccccaat
      301 aaatatcttt ttctgggtgc cagatatcgg cacgtccgcc agcaaagccg aaggtcttta
      361 aacccatcga cgcgtaagca acggtgccgg ctaaaacaat taagtctgcc caagaaagtt
      421 tattgccata ttttttcttg ataggccaca ataagcgacg tgctttatca agattcacgt
      481 tatcgggcca gctattgagg ggcgcaaaac gttgattgcc cgtgccagcg ccgcctcggc
      541 catccgcaat gcggtaagtg cctgctgcgt gccaggccat acgaatcatc aagccgccat
      601 aatggcccca atccgcaggc caccagtctt ggctatccgt catcaatgcc tttaagtcat
      661 tttttactgc ggccaaatct agcgttttaa atgcctgggc ataatcaaaa tcttcgccca
      721 acgggttagt tttgctatcg tgctgatgca aaatatccag attaagtgca tttggccacc
      781 aatccgtatt tgtgttgctc gcaacggtat tgccaccgtg catgaccgga cactttccac
      841 tagaaacagc aggtttattt tccatggtaa aactccttta tcgttaaaac agaatacaag
      901 tgatggataa aatagcggct taaaaactgg cagcaatgtt tgccagtatc tccaccaaaa
      961 tttgattcgc cgattcaaaa ataattgtga acatgattgc tccattcatc catgccatca
     1021 tcagaccgtt attgatattt gatgcataaa acgtatttta gtgaggcgca tttgatttgc
     1081 ataattgtat gttttgatgc aattgattta taaaacttat caatgtatga tgtcagcatg
     1141 accttgaccg aactaaaatt tattgtggcg gtggcgcacg agcgtaattt caggcgcgcg
     1201 gcagaaaaat gttttgtgac acagcccgcc ttaagtctag ccattaaaaa gctggaagaa
     1261 gaattaaacg tggtgatatt tgagcgtagt cgcacggatg tcagtatgac agaaataggc
     1321 atcaaaatcg tagaacaggc atcgaagatc ttacaagaag ccacgcaatt aaaacaaatc
     1381 gcagaactgg gtaaaaatca gctcaaaggc ccgctgaagc taggcatgat tcactctgtt
     1441 gggccatatt tactgccaga aatcattcca attttacgaa aaatcgcccc agatatgccg
     1501 ctggaggtgg aagaaaacct caccgccaac ctagaaacgc aattaagagg cggcgtcatc
     1561 gatgtggcca ttattgcctt gccgttcgat gtgcctggtt tgcaataccg cgcgctctac
     1621 gatgaggatt ttgatgtagt ggtgccaagc aatcaccatt gggtacatcg aaaacacatt
     1681 aaacctgaag aattatcgaa cgaaaaggtt ttgctactga atgctggcca ctgctttagt
     1741 aaccaagtca cgcaagcttg cccagaactt tcacgcaagg gtgaggtctt gcaaggcaat
     1801 tcactcgaaa ctatccgcaa tatggtcgca tctaatttgg gcattagtgt tttgccgtgc
     1861 tcagccaccg ctgcgcgtta tcataatccg ctcattcagg tcattccatt ttcagccccc
     1921 gcacccacta gacgcattgc cattgcctgg cgcaaaagtt atatgcggat ggaagccgta
     1981 gaaaaaattg tgcaagccat acaagcggta catgcagatt ttatgcatct gctttaagta
     2041 aacacgtaat aagtggctac gcgggcgaat tacttcgttg cgcggtgctc gcaacctcgc
     2101 tgtacactgc gcactatctc ggtttctgcg cgccgtgcgc ctcgcacttc atcccgctcg
     2161 ctcacgcatg aagtgcttcc taaatgtaaa aattggcaat taaaccacgc taaaaagtcg
     2221 tttaaaaatc acgaaaaccc cccgagaaaa aaattatgct ctgtgagcct ttatttataa
     2281 ggcttccaga gcatccaaaa aggttgtgct ttaaatgtgc gtggttatta gtgatttttt
     2341 agtatgtcac cagtgcgctg tagcccgaaa cttcttcgcc attggtggtg atattgagct
     2401 taattttaac ttgttctcca ggcccaatgc cgttttcaac cgctgtattt tgcggtaaat
     2461 attcagcggg ttttaaggtg cggcgtagtt tggggttatc gtcagcatct ttgagcgtaa
     2521 gttctaaatt cgggtaagtt tgcgcaaaac tcgcctgatt aatcaacgtg ctggtgagac
     2581 gcattaagcc agcatattct gcatcttctt ctattccaaa atcatcaata aaaatatatt
     2641 caattttttt cggcaatgta atttcgcagc caattttttc gcaagcactt accagatacg
     2701 gtttactgtt ggggtaataa ataggaattt cattgcgcaa aaaataaacg ctttggccaa
     2761 tggcagcaag ccctaaaagc gttgtaacgc aaaacaaaag ccaagtagag gctttgctgc
     2821 gcggtgcttt ttttttgtaa aatttagcat ccaccgataa gtttgaaatg ggcttagggg
     2881 tttcgactgt ggcctcaggt tctagcggaa cctcgctaaa acttggggcc agcgcgccgt
     2941 agtcaatcac ttcagcgata ttgtcttcat ccacaatatc aggtgtatca ggaacggtag
     3001 gttcggcagg ggcattgttc agaataggtg aactgatggt tgcttggcta acttcgtgtt
     3061 caatatcgtc aataatcgtg ggccatgcat tttcattatc ggatggtgta tcagtgtcaa
     3121 catcatgatc atgaaaagaa gcagaaacgg cataaaccgc acttatggaa acgtctgtgg
     3181 gctgaaaatt atccgttgaa acagaaagca cttcttgcgt ggcatctgca atgccctcgt
     3241 tgggtatggc ctcaaccatg tgcattgttg cgtcaaacac atgcaggcag tgaccgcaac
     3301 gtactttacc ctgatactgc ttgagctgtt ttttagagac aacaaactgc gtttggcagt
     3361 ttgggcagtt ggtaatattg ctcattaaca gatctacttt ttagtgccag tgagcaacac
     3421 ccaagcatcc atcgtttgtg ggctgtccat gttgaaccag tctgcgtaaa ttgcactcac
     3481 atcggcagcc tgttctttta aaatgcctga caaggcaatt ttaccgccag atttacatga
     3541 attcgcgagt gctggtgcca acacactcag tgcgcttgat aaaatattgg ccaccacaat
     3601 atcaaatgct ttagattgat aaaggctggc gtgcaaaaaa gatgcttgca cctgattttg
     3661 ttcggcgtta tataggctcg attgaatcgc ctgtgggtca atatcggtac ccaccacttc
     3721 tgcagcgccc agttttttag ccgcaattgc caaaatgcct gaaccgcagc cataatctaa
     3781 tacggaatta tttggctgaa cagtcttttt gagccatgca aggcataaat gcgtggttgg
     3841 gtggctacct gtaccaaatg ccaagccagg gtctaataca atgttgagtg catttgcgtt
     3901 tggcgcatta tgccaagtag gtacaatcca taaatcatca gtgattttaa tggggtcaaa
     3961 ctgtgattgc gttgcacgca cccagtcctg ctcttcaatg acttcagtag aatacgtcag
     4021 gtaattgagt ttagttttac gctgcaagtc cgccacaatg gccgccacat caacatcatc
     4081 atcaaacagg gcgctcacca agttttgttg ccaaatacca ggtggcgggt caccaggttc
     4141 gccaaaaata ggttgctcat ccaaagtgtc ggcatttgca tcttcaataa tggctgatag
     4201 cgcaccctgc gccatcagtg tatcactaat taaatcagcc gtattatctt gcgctgcaat
     4261 ttttaaagaa atccaagcca tgttcaacct atatatttaa agcgactata gttaaagtaa
     4321 caagcgcttg aaaatgacct aactttattt attgtaaatg ccaagctttt gttcaagata
     4381 atgaatgctc gtgccacccg catgaaaagc agcatctgcc attaaatcag aatgaagcgc
     4441 gatattagag ctaatacccg taatcatcat ttcagagagc gcaatgcgca tacgcgctat
     4501 cgcttggtca cgcgtatcac cataagtaat gagcttgcca atcattgaat cgtaatgcgg
     4561 tggcacagta tagccgccat aagcatgcgt atcgacacgt acgcccgggc cgccaggcat
     4621 atgaaaacgg ttaatggtgc cgggagacgg cacaaatgag tacgggtctt cagcattgat
     4681 acggcattca atcgcatggc cacgcaattg aatgtctttt tggcggaatt taagtttttc
     4741 accagacgcg acaaaaattt gctgctgtac caaatcaatg ccggtaatca tttctgtgac
     4801 ggtatgctct acctgcaaac gcgtgttcat ttcaatgaaa taaaactcgc cgttttcata
     4861 caaaaattca aacgtcccag caccacgata cttaattttg cggcaggctt cagcgcagcg
     4921 ttcaccaatt ttgtcacgca atttagggtt taataatggt gcgggcgctt cttcaataat
     4981 tttttgatgg cggcgctgca tagagcaatc gcgctcaccc aaaaataccg cgttaccgtg
     5041 ctggtcagcc aatacttgta tttcaatatg acgtggtttt tctaagtatt tttccatgta
     5101 aaccacgggg ttgccaaaag ccgcctgtgc ctccgctttg gtcatattga ctgaggcaag
     5161 taaagccgct tccgtgtgta ccacgcgcat gccgcgcccg ccaccgccac cggccgcctt
     5221 aataatgaca gggtagccga tttgcttggc tattttcatg acttcagcag ggtcttcagg
     5281 cagtgcgcct tcagaacccg gaacgcaagg cacgccagct tttctcatgg catttttagc
     5341 tgatacttta tcgcccatta aacgaatagt ttcagcgcgc gggccaataa agataaagcc
     5401 actttgttct acacggtcag caaaatcggc attttcagat aaaaagccat agcccggatg
     5461 aatcgcctgc gcatctgtga cttcagccgc gctgatgacg gctgggatgt tcaaatagct
     5521 ttggctagag ggtgctgggc caatacagac agattcatca gccagtttta cgtatttggc
     5581 ttc
//
LOCUS       DAOQSI010000130         5550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_14987_length_5550_cov_6.776342, whole genome shotgun sequence.
ACCESSION   DAOQSI010000130 DAOQSI010000000
VERSION     DAOQSI010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 5550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5550
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14987_length_5550_cov_6.776342"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="PKL42_08160"
     CDS             complement(<1..565)
                     /locus_tag="PKL42_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNU67087.1"
                     /translation="MQSPLILTTLFMGLAGGPHCIAMCGAACASLQHGQTNSLQIWKF
                     HAGRLLGYATLGAVAAGSVKSLAWFSGQTQSLHPLWTFFHVLVLAWGLVLLIYARQPI
                     WVEHFGRNIWARVRQLSHLPGGVFLTGILWALMPCGLLYSALLVASLNANPLNGALSM
                     ASFALGTSVSLFVGPWLWLKLKNGSQWV"
     gene            complement(674..2029)
                     /gene="hemN"
                     /locus_tag="PKL42_08165"
     CDS             complement(674..2029)
                     /gene="hemN"
                     /locus_tag="PKL42_08165"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HNU67088.1"
                     /translation="MLQKYDVSGPRYTSYPTADRFVEAFTEEAYQLALAQRRLSGASL
                     PLSIYVHIPFCESLCFFCACNKIVTKHHERSAEYLSYLSREIDLHVAHLGAGQTISQL
                     HLGGGSPTFFSDDELAELMALLKRSFVFAPGGEYSIEVDPRTVDEKRLAHLASLGFNR
                     LSFGVQDFDAEVQKAVHRIQPAEQVFDLVSAARSLGFDSVNVDLIYGLPKQTPASFAR
                     TLEQIVELKPDRIALYAYAHLPERFKPQRRIDAYELPAAADKIAMLANALSAFMRAGY
                     VYVGMDHFALPDDDLAVAKRQGRLHRNFQGYSTQPDCDLISFGVSAIGRVGNTYSQNA
                     KTLEEYYDAINLGHLPIVRGLALSRDDLVRRAVIMAIMCQGELQYEAIELAYMIDFKS
                     YFANELELLAEQENTGMVVLEENGLQVTDMGWFFVRAIAMLFDRYLQTDRNRARFSKI
                     I"
     gene            2523..3293
                     /gene="fnr"
                     /locus_tag="PKL42_08170"
     CDS             2523..3293
                     /gene="fnr"
                     /locus_tag="PKL42_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HNU67089.1"
                     /translation="MNLDISSDTPQDLAAPAKPLPTIKVACSNCNLRELCMPIGFNPE
                     DLHRLDEVVEKRRKIKQGEKLFGTGDVFKSLYAIRTGFFKTCVSTEDGREQVTGFQMA
                     GEIMGLDGIVSDHHNCSAIALEDAEVCVMPFADVEDLSREFPVLQRHVHKIMSREIVR
                     ENGVMMLLGNMRAEERLAAFLLNLVQRLHARGFSQSELTLRMTRKEIGSYLGMKLETV
                     SRTFSKFSDEGIIEVKQRYVKILNPDALKKIFNAQTCA"
     gene            3324..4172
                     /locus_tag="PKL42_08175"
     CDS             3324..4172
                     /locus_tag="PKL42_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNU67090.1"
                     /translation="MPKNKFLTVVDHNRDVSGMKYVYPVVSRRAGGVSIGINLNTNNA
                     CNWRCIYCSVPNLSRGTPPPIEMHVLENELRTFLNDVMHGDFMQIHVAEADRTLMDIA
                     FSGNGEPTSAKEFPAVLKLVENILRDFNLLENIKVRLITNGSLMDKADILTSIRHLAE
                     CNGEVWFKLDAGTKEGIARINDVNLNPQSHLERLKKCAAACPTYIQTCMFAIDGQPPT
                     EQAILDYLTLISQVKEVVAGVHLYGLARPSYQVEAARLGRLSEDWLENVASRIRELGL
                     KVYVNP"
     gene            complement(4169..5347)
                     /gene="ubiM"
                     /locus_tag="PKL42_08180"
     CDS             complement(4169..5347)
                     /gene="ubiM"
                     /locus_tag="PKL42_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HNU67091.1"
                     /translation="MTTHVDIAIIGAGPAGLCFAKSLAETGLKVMLIERQSEAAIADP
                     AFDGREIAVTRHSAKLMQALGLWEHIDAQAISPLRDARVLNGASLFALNFSHSETKQT
                     ELGYLISNHHIRKAAYEAVKGCPAIKLHTDAQVTSIQAVDDSISVTLADGEMIQAKLL
                     VGADSRFSETRRAMGIAADMHDFGKTMMVCVMEHTVPHEHTAWEWFDYGQTLALLPMN
                     GMRSSVVVTLPPSEITRLMHAPTDVFNQDMATRFQHRLGAMQLVSTRHAYPLVTAYAK
                     RMVGQRFALVGDAAVGMHPVTAHGFNFGLKGIETLSSEIKLAVAQGRDIGNNSQLMRF
                     ESAHRRNTRPLFMATHAIAKLYTSEILPARILRAGALRLGSRVKPFKHAVGAMLADAR
                     "
BASE COUNT         1522 a         1346 c         1235 g         1447 t
ORIGIN      
        1 tgacccactg gctaccgttt ttaagtttta gccataacca cgggcccaca aataatgaaa
       61 cgctggtgcc tagtgcaaag ctggccatgc taagtgcgcc gtttagaggg ttggcattga
      121 gtgaggccac caataaggct gaatataata gtccgcaagg catcagcgcc cacaatatgc
      181 ccgttaaaaa aacaccgccc ggtaagtgag atagctgacg aacacgcgcc caaatattgc
      241 gcccaaaatg ctctacccat atgggttggc gggcgtaaat tagcaacacc aaaccccatg
      301 ccagtaccag cacatgaaaa aatgtccata atggatgcag cgattgtgtt tggccagaaa
      361 accaagccaa acttttcact gaacctgccg ccaccgcacc cagcgtggcg tagccgagta
      421 atctacctgc atgaaacttc caaatttgta ggctattcgt ttgcccatgt tgcaggctcg
      481 cacaggccgc accgcacatg gcgatgcaat gcgggccgcc tgctaggccc atgaataatg
      541 tagtgagaat caatggtgat tgcatgatgc tgctatctta catgatagcg gccaatactg
      601 aagcgactgc ccgccataat ttagcgttcg ccctgcgcct atcgaaagac tcagggcgaa
      661 cggatagacc tagttaaata attttactaa aacgcgcgcg gttacggtct gtttgcaaat
      721 agcggtcaaa caacatggca attgcccgca caaaaaacca acccatgtca gttacttgca
      781 aaccgttttc ttcaagcacc accatgccgg tgttttcttg ctctgccagc agttcaagct
      841 cattggcgaa gtagctctta aaatcaatca tataggcgag ttcaatggcc tcgtactgca
      901 gttcaccttg gcacataatc gccataatca cagcgcggcg caccaaatca tcacgcgata
      961 atgctaaacc tcgcacaatc ggcaagtggc ctaaattgat tgcatcgtaa tattcttcaa
     1021 gcgtttttgc gttttggcta taagtgttcc ccacgcggcc aatggctgaa acgccaaagc
     1081 taattaaatc gcaatctggt tgcgtgctat agccctgaaa attacggtgc aatctgccct
     1141 ggcgtttggc cacggccaag tcatcatcag gtaaggcaaa gtgatccatc cccacataca
     1201 cataaccggc gcgcataaag gctgatagcg catttgcaag catggcgatt ttatcagctg
     1261 cggctggcaa ttcgtaagca tctatacggc gctgcggctt aaaacgctct ggtaaatgcg
     1321 catacgcata caacgcaatt ctatcgggtt ttaattcaac aatttgttcc agcgtgcgtg
     1381 caaacgatgc cggcgtttgc ttaggcaaac cgtaaatcaa atccacattc actgaatcaa
     1441 aacccaagct gcgtgcggcc gaaaccaaat caaacacttg ttctgcgggt tgaatacgat
     1501 gcacggcttt ttggacttct gcgtcaaaat cctgtacgcc aaaactcaag cgattaaacc
     1561 ccagtgacgc taaatgcgcc aagcgttttt catccacggt acgcgggtca acctcaatcg
     1621 agtattcacc acctggtgca aacacaaagc tacgcttcag tagcgccatg agttcagcca
     1681 attcatcatc tgagaaaaat gtgggggaac cgccgcctaa gtgtaattgc gagatagttt
     1741 ggccagcacc taaatgtgca acatgcaaat caatttcacg gctaagatag cttaagtatt
     1801 cagcgctacg ctcatggtgc ttggttacga ttttattaca ggcgcagaaa aaacacaggg
     1861 actcgcaaaa tggaatatgc acataaatgg acaatggcaa tgaagcacca cttaagcgtc
     1921 gctgtgcaag tgctaactga tacgcctctt cagtaaaggc ttctacaaat cggtctgccg
     1981 ttgggtatga ggtataacga gggcctgaga catcatattt ttgcaacatt tccgccgtaa
     2041 cacagggtaa agcggtagcg gctgaagtga atccagccgc acttgtttta tttgcgttta
     2101 atgcattatt tttcatctcg ataccactcc taatctagcg tgccaatatg ccagttttct
     2161 aaataaaaaa ctttgatgca tatcaaatga atttacatca ccttttgtac cagtctgcaa
     2221 gcctttattt ataaggctcg cagggcagta atttttttac gtgtaatttt gtcgcatcat
     2281 agtgatattg aatttaacta tttttacttt tggttttagc acattagcct agcctatatc
     2341 tttattttgc tatacgtcat ggtgattcgc tagcaaatct tctgcttaac cgatggtttc
     2401 attacgattg taatcctttt cattttcatc aattaaccat gtatatggct agcatcaaag
     2461 ctacttacgc gttatgctta tatagttgac gactttgtca ttagcccgtg tatcgcaaaa
     2521 ccatgaacct agacatttct tcagacacac cgcaggattt agcagcgccc gctaagccac
     2581 ttcctaccat taaagtagcg tgttccaatt gcaaccttcg tgagctttgt atgccaattg
     2641 gttttaatcc agaagatttg caccggttag atgaagtggt tgaaaagcgt cgaaaaatta
     2701 agcaaggcga aaaactattt ggtactggtg acgtgtttaa atcactttat gccattcgta
     2761 cgggattttt taaaacctgc gtttcaaccg aagatggccg cgagcaagtc accggcttcc
     2821 aaatggcggg cgaaattatg ggcttagatg gcattgtcag tgaccatcac aactgtagcg
     2881 ccatcgcact tgaagatgcc gaagtctgcg tgatgccttt tgccgatgtt gaagatttat
     2941 cgcgcgaatt tccagtgttg caacggcatg tgcataaaat catgagtcgt gaaattgtgc
     3001 gtgaaaacgg cgtgatgatg ctgctaggca atatgcgggc tgaggagcgt ttggccgcat
     3061 ttctattaaa tttggtgcag cgcttacatg cgcgcggttt ttcccaatct gaactcaccc
     3121 taagaatgac gcgtaaagaa attggtagtt acttgggcat gaaattagaa accgttagcc
     3181 gtacattttc aaagtttagc gatgaaggta ttattgaagt gaaacagcgc tacgtgaaaa
     3241 tcctcaaccc tgacgcactg aaaaaaatat ttaacgctca aacttgcgct taaacattca
     3301 ggcgaatcgt tttttattcg atgatgccca aaaataaatt tcttactgtc gttgaccaca
     3361 accgcgatgt cagcggtatg aagtacgttt accccgttgt ttcgcgccgc gctggtggcg
     3421 tatccattgg catcaacctc aacacaaaca atgcctgtaa ctggcgctgt atttattgca
     3481 gcgtgccaaa cctcagccgt ggcacaccgc cgccgattga gatgcatgtg cttgaaaatg
     3541 aacttcgcac atttctaaac gatgtgatgc atggcgattt tatgcaaatc cacgtggcag
     3601 aagctgatcg aacattaatg gacattgctt tctctggcaa cggtgagcca acgagtgcta
     3661 aagaatttcc agccgtacta aaactagtag aaaatatact gcgcgacttt aatttgcttg
     3721 aaaacattaa agtgaggctc atcaccaatg gcagcctaat ggacaaggcc gacattttaa
     3781 cgagtatccg tcatcttgct gaatgtaatg gtgaggtttg gtttaaactg gacgcgggaa
     3841 caaaagaggg catcgcacgc attaatgatg tgaatcttaa cccacaaagc catcttgaac
     3901 gcctgaaaaa atgtgcagca gcctgcccga cctatattca aacgtgtatg tttgccattg
     3961 atgggcaacc gccaactgag caggctattt tggattactt aacgttaatc agtcaggtta
     4021 aagaggtggt tgcgggtgta cacctttatg gcttggcccg tccatcttac caagttgagg
     4081 ccgcaaggtt aggccgcctt tctgaagatt ggcttgagaa cgtcgcaagc cgcatacgtg
     4141 aacttggcct taaggtttac gtcaacccct agcgcgcatc tgccagcatt gcaccgaccg
     4201 cgtgcttgaa tggcttgacg cgactgccta agcgtaacgc gcctgcacgt aatattctgg
     4261 caggtaaaat ttcactggta tataactttg caatggcatg cgttgccata aaaagcgggc
     4321 gcgtgttacg ccgatgcgcg ctttcaaaac gcattaactg gctgttgttg ccaatatcgc
     4381 gcccttgagc aacggccaat ttaatttcag aagataaagt ctcaatgcct tttaaaccaa
     4441 aattaaagcc atgcgcagtc actgggtgca tgccaaccgc ggcatcgcct accagcgcaa
     4501 accgctggcc gaccattctt tttgcgtaag cggtcaccag cggataagcg tgccgagttg
     4561 aaacaagttg catggcgccc aatcgatgtt gaaagcgagt cgccatgtct tggttgaaaa
     4621 catccgtcgg tgcgtgcatt aaacgtgtaa tttcactggg aggcaaggtg acgacaaccg
     4681 aagagcgcat gccgttcatc ggcaataacg cgagcgtttg cccataatca aaccactccc
     4741 aggccgtatg ttcgtgcgga acagtgtgct ccatcacgca taccatcatg gttttgccaa
     4801 aatcgtgcat gtcagcagca atacccatcg cacggcgcgt ttcagaaaaa cggctatctg
     4861 cacccactaa taatttcgcc tgtatcattt caccatcagc tagcgtcaca ctaatactgt
     4921 catcaacggc ttgaatgctc gtcacttgcg catctgtgtg caacttgatt gcggggcagc
     4981 ctttcactgc ctcataagcg gctttgcgaa tatggtgatt agaaatgagg taacccaact
     5041 cagtctgttt ggtttcgctg tggctaaaat tgagcgcgaa taacgaagcg ccattcaaca
     5101 cgcgcgcgtc ccgtagaggc gaaatagctt gtgcatcaat gtgttcccac aagcccaacg
     5161 cctgcatgag tttggcagaa tgacgcgtca cagcaatttc acggccgtca aaagctgggt
     5221 ctgcaatggc tgcttcactt tgccgctcaa ttagcatcac tttcagacct gtttcagcaa
     5281 gcgatttggc aaaacataag cccgcgggcc cagcaccaat aattgcaata tccacgtgag
     5341 ttgtcatcgc caccactttc agtataaaaa caaaaattgg gtttacatta ttgcagaatg
     5401 ctaatccgct taaccataca gttttgtgag gcggattttt cgctaaatat ccctaaatat
     5461 cactacaacc tttttcgatg ctctgtgagc cttataaata aaggctcaca gagcattatt
     5521 ttttcctcgg tgcaaatttt agtgattttc
//
LOCUS       DAOQSI010000131         5530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_15062_length_5530_cov_5.080000, whole genome shotgun sequence.
ACCESSION   DAOQSI010000131 DAOQSI010000000
VERSION     DAOQSI010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15062_length_5530_cov_5.080000"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..202)
                     /locus_tag="PKL42_08185"
     tRNA            complement(129..202)
                     /locus_tag="PKL42_08185"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(168..170),aa:Cys,seq:gca)
     gene            complement(338..413)
                     /locus_tag="PKL42_08190"
     tRNA            complement(338..413)
                     /locus_tag="PKL42_08190"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(378..380),aa:Gly,seq:gcc)
     gene            complement(497..1093)
                     /gene="pgsA"
                     /locus_tag="PKL42_08195"
     CDS             complement(497..1093)
                     /gene="pgsA"
                     /locus_tag="PKL42_08195"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNU67092.1"
                     /translation="MKMNIPTMFTWLRILLIPVFVGAFYWPDNLHQLGLKPSHLVNVT
                     ATAIFAIAAITDWLDGYLARKLNQTSAFGAFLDPVADKLMVAAALIVLIKFDRVGEIV
                     SLIIIGREIAISALREWMAGQGQGSSVGVATIGKIKTTAQMLAILLLLYWDNIDLGEL
                     GVFPTKVVGNVLILVAAFLTLLSMAYYLKIALPQIKAK"
     gene            complement(1126..2925)
                     /gene="uvrC"
                     /locus_tag="PKL42_08200"
     CDS             complement(1126..2925)
                     /gene="uvrC"
                     /locus_tag="PKL42_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNU67093.1"
                     /translation="MFDPKPILKNLPNLPGVYRMINSTDEVIYVGKAKDLKKRVSSYF
                     NKNLPSPRTRMMVSNIVKIETTVTHNEAEALLLENNLIKGLMPRYNVLFRDDKSYPYI
                     TLTSDQFSRLAFHRGTQRKGNQYFGPFPNSVAVRESIQLLQKVFKLRTCENTVFANRS
                     RPCLQHQIERCTAPCVGLISDEDYRNDVHQAAMFLQGKTNEVINALGEQMNTAAANQE
                     YEMAVVFRDRMQALRQVQAKQFVSDFNVSDADVVACAELQGQHCINLVMIRGGRHLGD
                     RSYMPKNSDGETLEASMSAFLAQHYTAQNTPPLIVIGIKIETAVLEEVLSQQAGRKVK
                     INTNPIGDKRVWLKMAQTNAELALGQRAATSANQEAKLMALREALHLPESTERIECFD
                     ISHTMGEATIGSCVVFDRGDMQNSEYRRYNVTGITPGDDYAAMRDVLTRRYKKVAAGE
                     GVRPDLIFIDGGKGQLSVAVEVMAEVGLEDILLVGIAKGEARKPGLETMIFSDTGEML
                     NLEKDNKGLHLLQQIRDEAHRFAITGHRAKRAKARLQSSLEDIEGVGAKRRKALLTRF
                     GGLDGVKNASIDELSQVDGISLSLAEKIYGEFH"
     gene            complement(3174..3356)
                     /locus_tag="PKL42_08205"
     CDS             complement(3174..3356)
                     /locus_tag="PKL42_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HNU67094.1"
                     /translation="MPYVNVKLAGNVTKEQKAQIAKEFTDTLERVAHKPKSYTYITFE
                     EVSYEDWAIGGKLLDE"
     gene            complement(3375..4046)
                     /locus_tag="PKL42_08210"
     CDS             complement(3375..4046)
                     /locus_tag="PKL42_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNU67095.1"
                     /translation="MTNPRQHAHDDHSDHDDHDHYLIPFILIFTFAIVEAVGSWYTGS
                     LALLSDAGHMFTDVTALGLAWLAGILSKKPKVARHHSGVSYVELAVSIFNAITMLGVI
                     AYVVFEAIVRFKTPHIVQGAGLTIIATLGLIVNLIVAKQLHHQAVHHAETLNNRAAFL
                     HVIGDLLGSIAAVAAGVVIYFTGWMLIDPILSLLISLLLLVFTLNLIRDIWRTMHGKA
                     TKYHH"
     gene            4159..5007
                     /gene="rlmJ"
                     /locus_tag="PKL42_08215"
     CDS             4159..5007
                     /gene="rlmJ"
                     /locus_tag="PKL42_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HNU67096.1"
                     /translation="MLSYRHAFHAGNHADVLKHYVLTLVLAYMKQKDKPFWYIDTHAG
                     AGLYSLTNGYAKQNAEFEQGIAKLMAAQRLPQPLADFVTLIKSHNSNANALQFYPGSP
                     IIAQDFLRADDKMRLFELHPNDCKLLIENFRGQGKQVKIEMQDGFNGIKACLPPPPRR
                     AVILIDPPYENKQDYQHVVDAIKDSLKRFATGTYMIWYPILQRPEPDEMIEQLHRLNL
                     PNWLHVAMTIQAPATEGFGMHGSGLFIINPPWSLPKVLTETMPILTNLLALDASADFF
                     VDSKIA"
     gene            5212..>5530
                     /locus_tag="PKL42_08220"
     CDS             5212..>5530
                     /locus_tag="PKL42_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNU67097.1"
                     /translation="MLLINQIDGAYAHFKQLFTERAKMPFDPNDENALLNPQQTIVII
                     DDEFTTRTILRETVLGICDSIVVESFSNPVSALEWLLVNEPDLILLDYKMKEMSGYEL
                     LCAL"
BASE COUNT         1569 a         1311 c         1116 g         1534 t
ORIGIN      
        1 cgagagattt taagtctctt gtgtctacca atttcaccac ccgggctaag ggtgatattt
       61 ctatctcata cagcacttct attgcggcgc gtattctacc actttatgca aaaatactgc
      121 acaatatttg gaggcgcgaa ccagagtcga actggtctaa acggctttgc aggccgcggc
      181 ataaccgctt tgctatcgcg ccattttcgt aaatgcaatt acattggcta ctttagttat
      241 tagcttaaac taaaacggcg aaagcataga tactttcgcc gttgtatgcc attttaccaa
      301 tttatgaaat agttacgtat cataaatttt aaattcttgg agcgggaaac gagactcgaa
      361 ctcgcgacct ataccttggc aaggtatcgc tctaccagct gagctattcc cgcgtcgtta
      421 agacaggcgc cattatagcg attttcacaa taccgtcaag actaaaacgc gatagttgat
      481 acttatttat tttgttttat ttcgccttaa tttgcggtaa agcaattttt aagtaataag
      541 ccattgatag caatgttaaa aatgcggcca cgagaattaa gacattgcca acaacttttg
      601 ttgggaaaac gcctaactca cctaaatcaa tattatccca ataaagcaaa agcaaaatgg
      661 ctaacatttg tgcagttgtt ttaatcttgc ctatcgtcgc cacgccaacg ctactgccct
      721 gcccttgccc ggccatccat tcacgtaggg cgctaatcgc aatttctcga ccaataataa
      781 tcaaagagac aatttcaccg acccgatcaa atttaatcag cacaatcagc gcggcagcca
      841 ccatcaactt atcggcaacg gggtctaaaa acgcaccaaa agctgaggtt tgattcaatt
      901 tgcgcgccaa atagccgtcc aaccaatcgg taatcgctgc aatcgcaaaa atagccgttg
      961 cggtcacgtt aaccaaatgt gacggcttta accccaattg atgtaaatta tctggccagt
     1021 aaaaagcacc aacaaagacg gggattaaca agatacgcaa ccatgtaaac atggtaggaa
     1081 tattcatctt catttactgc acccacactt caaaacggcc tgccactaat gaaactcccc
     1141 atatattttc tctgccaggc ttaggctaat gccatccact tgactcagtt cgtcaatact
     1201 agcatttttt acgccgtcta agccaccaaa acgcgttaaa agcgctttac ggcgttttgc
     1261 gccaacgcct tcaatatcct ctaagctaga ctgcaaacgc gctttagcac gctttgcacg
     1321 atgcccagtg atggcaaatc ggtgcgcctc atctcggatt tgttgtagaa gatgcaggcc
     1381 tttgttatct ttttctaaat tgagcatttc accggtgtct gaaaatatca tggtttcaag
     1441 cccaggtttt cgtgcttccc ctttggcgat gcccaccagc aaaatatctt ctaaacccac
     1501 ttcagccatc acttcgacag caacacttaa ttggcctttg ccaccgtcaa taaagattaa
     1561 atctggccgc acaccttccc ctgctgccac ttttttatag cggcgtgtca aaacatcgcg
     1621 catagctgcg taatcatcac ctggcgtaat gcccgtgacg ttataacgcc gatattcgct
     1681 attttgcatg tcgccccggt caaacactac gcagctccca atggttgctt cgcccatggt
     1741 gtggctaata tcaaaacact caatacgctc agtgctttca ggcaaatgca gtgcttcacg
     1801 caaggccatt aacttcgctt cttgatttgc tgaagttgct gcacgttgcc ccagcgctaa
     1861 ttcagcattg gtttgcgcca tttttagcca tacgcgctta tcgccgattg ggttggtgtt
     1921 aattttaact tttcgacctg cttgctgact taagacttct tcaagcacag cggtttcaat
     1981 tttaatacca atcacaatca acggtggcgt attctgcgcg gtgtaatgtt gtgctaaaaa
     2041 ggcagacata ctcgcttcta gcgtttcgcc gtctgaattt tttggcatat aacttctatc
     2101 gcccaaatgc cgcccgccgc ggatcatcac caaattaatg caatgctggc cttgcaattc
     2161 tgcacaggca accacatcgg catcggatac attaaagtca ctcacaaact gcttggcttg
     2221 cacttgccga agcgcttgca tgcggtcacg aaacaccacg gccatttcat attcttgatt
     2281 cgccgctgcg gtgttcattt gctcacctag cgcattgata acctcattgg ttttgccctg
     2341 caaaaacatg gcggcctgat gcacatcatt gcgataatct tcatctgaaa tcaagcccac
     2401 acaaggcgcg gtacagcgct ctatctgatg ttgcaaacaa gggcggctac ggttggcaaa
     2461 cacggtgttt tcacatgtac gcagtttgaa tactttttgc aaaagctgaa tactttcgcg
     2521 cacggccact gaattgggaa aaggcccaaa atactggtta cctttacgct gtgtgccacg
     2581 atgaaacgcc aagcgcgaaa attggtcact cgtcagcgta atatacgggt aagacttatc
     2641 atcgcgaaac aacacgttat aacgtggcat taagccttta atcaggttat tttccagcaa
     2701 taaagcttct gcttcattgt gcgtgacggt ggtctcaatt ttgacgatat tactcaccat
     2761 catccgcgtg cgcgggctag gcaagttttt attgaagtaa cttgaaacgc gctttttgag
     2821 gtctttggct ttgcccacat aaatcacctc atctgtggag ttaatcatac gatacacgcc
     2881 gggcaggttt ggcagattct ttaaaatagg ctttggatca aacatggcaa tattttaacc
     2941 cagattttcc attaggactt gaagtggcaa agccgcacag tttgatggat tttttgtgca
     3001 gattttatgg tcaatagtcg ttctattgac agaaaatctg cacaaaaatg acgcaaaatg
     3061 ggcaagttga cattcaagta gatgcaaaat cgcctaatgt aaaatctggg ttcaacacca
     3121 gcggcatcat tccacgccat cggtgaaatg atgcgccatt gagcaattta gacttactcg
     3181 tctagcagct ttccgcctat cgcccaatcc tcataagaca cttcttcaaa ggtaatgtag
     3241 gtataagatt tcggcttatg ggcaacgcgt tccaaagtgt cagtaaactc tttggcgatt
     3301 tgcgcttttt gttctttggt cacatttcct gcaagtttta cattgacgta gggcataatg
     3361 ttctcctgta aaaattagtg gtggtattta gttgcttttc cgtgcatggt acgccaaata
     3421 tcgcgtatca gatttaaggt aaacactagc aaaagcagtg aaatgagcag cgataaaatc
     3481 gggtcaatca gcatccaacc cgtaaagtaa atcacaaccc ctgccgcaac ggccgctatc
     3541 gagcctaaca aatcccctat tacatgcaaa aatgctgcgc ggttattcaa ggtttctgca
     3601 tggtgcacgg cctgatggtg caactgcttg gcaacaatta aattcacaat caaaccaagg
     3661 gttgcaatga tggttagtcc cgcgccttgc acgatatgcg gtgttttaaa tcgtacaatg
     3721 gcttcaaaca ccacataagc aatcacaccc agcatggtga tggcattaaa aatagaaacg
     3781 gctaactcta cataactcac ccctgagtga tgccttgcca ctttgggttt tttagaaagt
     3841 atgcctgcga gccacgctag gccgagtgca gtaacatcgg taaacatgtg gccagcatca
     3901 cttaataatg ccaaagaacc cgtgtaccaa ctgccaactg cctcaacaat tgcaaaagta
     3961 aaaatcagta taaatggaat caggtagtgg tcatgatcat catggtcgct gtggtcgtcg
     4021 tgtgcatgtt ggcgtggatt tgtcataggt gaaatgctaa ccgaaaagtg agtttgttga
     4081 gcatttttat cgcgaaaaaa tgtttggtct agaagctaaa gctataatgt cgccattcaa
     4141 tattttttag cagacattat gcttagttac cgtcacgcct tccacgcagg caaccatgcc
     4201 gatgtactta aacactacgt gctgacgctg gtgctggctt acatgaagca aaaagataag
     4261 ccgttttggt atattgatac ccatgcaggc gctggcttgt acagtttaac gaacggctac
     4321 gccaagcaaa atgcagagtt tgagcaaggt attgccaaac tgatggcggc gcaacgcttg
     4381 ccgcaaccac tggctgactt tgttactcta attaaaagcc acaattcaaa tgctaatgcg
     4441 cttcagtttt acccaggctc acccatcatt gcgcaagatt ttttgcgtgc tgacgacaaa
     4501 atgcggctgt ttgaactgca cccaaatgac tgcaaattat tgattgaaaa ttttaggggt
     4561 caaggcaagc aagtcaaaat tgaaatgcaa gatggtttta acggcattaa agcttgcctg
     4621 ccgccaccgc cacgtcgtgc agtgattttg atagatccgc catatgaaaa taaacaagat
     4681 taccagcatg tggttgatgc gattaaagac agcctgaaac gctttgcaac tggcacttat
     4741 atgatttggt accccatttt gcagcgccct gagccagatg aaatgattga acagttgcat
     4801 cgcctcaatc tgcccaattg gctgcatgtg gccatgacga tacaagcacc agccactgaa
     4861 ggttttggca tgcatggcag tggtttattc atcattaatc caccctggtc gctgcctaaa
     4921 gtactgactg agactatgcc aatactcacc aatttacttg ctttagatgc aagtgcagac
     4981 ttttttgtgg atagcaaaat agcctgattt ataagatagc caatcactta aataacatcc
     5041 gccacacatc agttttttca ttaaagtcca acattgaaac agtgtcattt attgacattt
     5101 ttgttgagat tttggcaacc atcagtacaa aaaaagttaa aaaatcaaca ccaactttct
     5161 aacacaatga aatacaaggg aatttattaa aaaccacatt aggcacacat attgctttta
     5221 ataaatcaaa ttgatggtgc gtacgcgcac tttaagcaac tttttactga aagagcaaaa
     5281 atgccttttg acccgaatga cgaaaatgcg ctgctgaacc ctcagcaaac cattgtaatt
     5341 atcgacgacg aatttaccac cagaaccatt ctgcgcgaaa ccgtgctggg tatttgcgat
     5401 tctattgtgg ttgaatcatt ttcaaaccca gtctctgccc tagaatggct tttagtgaat
     5461 gagccagact tgattttgct cgattataaa atgaaagaaa tgtctggcta cgagttactc
     5521 tgcgcattaa
//
LOCUS       DAOQSI010000132         5530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_15063_length_5530_cov_4.502831, whole genome shotgun sequence.
ACCESSION   DAOQSI010000132 DAOQSI010000000
VERSION     DAOQSI010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15063_length_5530_cov_4.502831"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="PKL42_08225"
     CDS             <1..862
                     /locus_tag="PKL42_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SUV3 C-terminal domain-containing protein"
                     /protein_id="HNU67098.1"
                     /translation="IYGALSPEVRRTESERFCHGQADILVATDAIGMGLNLPIRRVIF
                     SNIHKFDGVASRPLNATEVRQIAGRAGRFGIYDIGYVSVLENDERIHIEHMLNMDDTS
                     DIQKLPISTQYEQLETIATQLHTRKIAEVLQFLQDRTRLSNPLFEQSSLNTQIEQAKL
                     VDINAPNMSLKDKFIFVCAPTSLNVAFEKDYYLLCLQSVALSNIRHLPKAPIWLEANS
                     PKHLEEAELLSQNLSLYAWLGFKFPQIFIEGDAVPALRKSISRYIENALLTQAGYGET
                     NREVEVKFLR"
     gene            complement(871..2094)
                     /locus_tag="PKL42_08230"
     CDS             complement(871..2094)
                     /locus_tag="PKL42_08230"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNU67099.1"
                     /translation="MATEDTIHQYNLSIFESYLKDVRTGTTSSMVSLLFLTVISFEAV
                     DGAMLSIWFALILITAAAKFLSYRYFDPNFDAHSLTKFENTHHLMQAFSGIAWGSSCF
                     FLLKSGDINGNESLVLNMLCVLVAYSAVVMSASSTGMLTFVTPVIASMLIYFATDLTH
                     YHWWFVATLLLAGASAIFGKTNKKHVLSQVESQLLNKRYIDELNRVKASVQRANLDLV
                     KKNEALVIAQERLRMLATNDELTQVFNRRHAMTYLEKVFAELRRRPDDFVVVLADIDH
                     FKSINDRYGHPAGDLVLRGFSKMLKEQLRQIDTVARFGGEEFLIMLPKTKELEAKAII
                     QRLCDAVAAQPYKLQGQSVKVTSSFGLAFFQVGDTIEKLIERADQALYHAKKSGRNTV
                     KTHYEADMAAYITAD"
     gene            complement(2285..3736)
                     /locus_tag="PKL42_08235"
     CDS             complement(2285..3736)
                     /locus_tag="PKL42_08235"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNU67100.1"
                     /translation="MLCLTLAMANIAHAENKIIASRVWPAQDFTRITLESNKPFVYKM
                     QIISNPDRVVLDIENVEFNQIIKSTNDRVLPTDPYIKQIRVARFQQNVVRLVVDLKDV
                     SKPNVFSLKPTGDYNHRLVLDIHPLHDPLMAMLAARDAATNAQANIPVDASQPSSEPQ
                     GSTEALPNLVSTTDSQPIAENAPTTDTTQIAEQSKLPETTKDTDVTTSDMPSADNIDN
                     KNVAIVPPDATKSDSYKPSVNKADSPAFRQITIAIDPGHGGEDPGAIGASGSREKDVT
                     LLIAKKLKEKIDAEPNMRAILTRDGDYFIPLHDRVIKARKLQADLFLSIHADAFTNPA
                     ARGSSVFALSEKGATSAGARYLAKKENESDLIGGVSLGGKDPVLAKTLLDLSQTATIN
                     DSLKLGKAVLMQIGEINKLHAKRVEQAGFAVLKSPDIPSILIETAFISNPEEERKLND
                     DAYQDKLVKSILTGIKKYFSTNPALAKTKIAQE"
     gene            complement(3769..4275)
                     /gene="tsaE"
                     /locus_tag="PKL42_08240"
     CDS             complement(3769..4275)
                     /gene="tsaE"
                     /locus_tag="PKL42_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNU67101.1"
                     /translation="MEHDFTLDLADEAATLQFAAILAKALVPNFVIYLHGDLGAGKTT
                     LVRGVMHALGFAGKVKSPTYTLVEMYDIELQPNSKCHLYHFDLYRFNDEEEWNAAGFR
                     DYFNARSVCIIEWPEKAQQVLPTPDIDIVFSIQSIGRKIQIIAHSVLGQQCLQAIISQ
                     AIHLQGKP"
     gene            4274..5356
                     /gene="queG"
                     /locus_tag="PKL42_08245"
     CDS             4274..5356
                     /gene="queG"
                     /locus_tag="PKL42_08245"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNU67102.1"
                     /translation="MTTHPMDFTQLSAAIKRWGIALGFSDIGITDTHLQTAETEHQVW
                     INKGFHGDMDYMAKHGTKRTRPAELVPNTTRIISARLDYLPPQSADSEAILQAPNQAF
                     ISRYALGRDYHKVMRKKLQKLCDNIQAALVNDAADAFEYRVFTDSAPVLEVAIAEKSG
                     LGWRGKHTLLINKDRGSWFFLGEIYTNLPLIIDAPASNHCGTCTSCIEVCPTQAITAP
                     YEVDARRCISYLTIELKTSIPIEFRPLIGNRIYGCDDCQLFCPWNKFAQITTEADFAI
                     KHGLDTITLLECFLWDEWTFKEKMAGSAIYRIGYPQWLRNVAVGLGNANTTPEIITAL
                     KAREFDENALVREHVVWALQQHLNKQ"
BASE COUNT         1581 a         1201 c         1169 g         1579 t
ORIGIN      
        1 catttatggc gccctttctc ccgaagtacg gcgtacggaa tctgagcgat tttgtcacgg
       61 ccaggcggat attctagtgg ccacggatgc cattggcatg gggttaaact tgccaatacg
      121 tcgcgttatt ttctctaaca ttcataaatt tgatggcgtc gcatctcgcc cattaaatgc
      181 aacagaagtc aggcaaattg ccggtcgcgc cggccgtttt ggcatttatg atattggcta
      241 tgtgagtgtt ttagaaaatg acgaacgtat tcatattgag catatgctca atatggatga
      301 tacgtctgac atacaaaaac tgccaatcag tacgcaatat gaacaactcg agaccattgc
      361 aacgcaacta catacgcgaa aaattgctga agttttacaa tttctacagg atagaaccag
      421 attgagtaac ccactttttg aacagtcttc actcaacacc caaattgagc aggctaaatt
      481 agtagatata aatgcgccaa atatgtcgtt aaaagacaag ttcatcttcg tatgtgcacc
      541 tacttctctc aacgtggctt tcgaaaaaga ctattattta ttatgtttgc aaagtgtagc
      601 tctatcaaac attcgacatt tgccaaaagc gcccatatgg ttggaagcta atagcccaaa
      661 acatttagaa gaagcagaat tactcagcca gaatctcagt ttgtatgctt ggcttgggtt
      721 taaatttccg cagatattta ttgaaggtga tgccgtgcct gcactcagaa aaagcatcag
      781 tcgctacatt gaaaacgcat tactgacaca agcaggttac ggtgaaacta accgagaagt
      841 agaagttaaa tttttacgct aaacacgcgc ctaatctgct gtaatatatg ctgccatatc
      901 cgcttcataa tgagttttaa ccgtatttct accagatttt ttcgcgtgat aaagcgcttg
      961 gtctgcccgt tcgattagtt tttcaattgt gtcacccacc tgaaaaaacg ccaatccaaa
     1021 ggacgaggtc acttttactg actggccttg aagtttgtag ggctgtgccg ctaccgcatc
     1081 gcataaacgc tgaataatcg ctttcgcttc caactctttg gtttttggta acataatcaa
     1141 aaactcttcc ccgccaaatc gcgcaacggt atctatttgg cgaagttgtt ctttcaacat
     1201 ttttgaaaaa ccacgcaaca ccaaatcacc tgctggatgc ccataacggt cgttaattga
     1261 tttgaaatga tcaatatctg ccagcaccac cacaaaatcg tcagggcggc gtcttaattc
     1321 agcaaacact ttttctaaat aagtcatggc gtggcgacga ttaaatacct gagtcaattc
     1381 atcattggta gccaacatgc gaagccgttc ctgcgcgatg actaacgcct catttttctt
     1441 gaccaaatcg aggttagcgc gctgcacact tgctttgaca cgatttaact cgtcaatata
     1501 gcgcttattc aacaactgac tttctacttg cgacaatacg tgttttttat tggttttacc
     1561 aaaaatagca ctcgcgcctg ccaataatag cgttgccaca aaccaccaat gataatgcgt
     1621 taaatccgtg gcaaaataaa tcagcataga agcaataacg ggtgtcacaa aagtcaacat
     1681 acctgtagaa gaagccgaca tgacgacagc tgaataagcc accaatacgc aaagcatgtt
     1741 gagtactaga ctttcattgc catttatgtc tccagacttt aataaaaaga aacaagatga
     1801 tccccatgcg atccctgaaa acgcttgcat gagatggtgg gtattctcaa atttcgttag
     1861 agagtgagca tcaaaatttg gatcgaagta tctgtaagat aaaaacttag ctgctgcggt
     1921 tatcaatatc aaggcaaacc aaatagagag cattgctccg tcaactgctt caaaagaaat
     1981 aacagttaaa aagagcaaag aaaccatgct tgaagttgtg ccggtgcgaa cgtctttaag
     2041 atagctctca aagatgctta aattgtactg atgaattgtg tcttctgtag ccatgaaatt
     2101 agatgtatgt gtaagttgtt atttattgtt aaatactttt aagcatgagt cgtgccaact
     2161 ctgcaacaat ttgtataatt cgtgaattat ttgaaaacga tggtgttcaa gcgataaaac
     2221 agcgctagat ttggactagt tgaacttaga aaccaagaaa catcaacttc caattggtct
     2281 gagtttattc ctgggcgatt ttagttttag ctaatgcagg attggttgaa aaatactttt
     2341 tgatgccagt taaaattgat ttcaccagtt tgtcctgata agcatcatcg ttcaacttgc
     2401 gctcttcttc agggttgctg ataaaagccg tctcaatcag aatggaggga atatcgggtg
     2461 attttaaaac ggcaaacccc gcctgttcta cacgttttgc atgtaattta ttaatctcgc
     2521 caatttgcat caaaacggct ttacccaatt ttaagctatc gttaatggtg gcagtttgtg
     2581 ataagtccag cagcgttttt gctaatactg ggtcttttcc acctaggctc acgcctccga
     2641 ttaagtcaga ttcattttct tttttcgcca aatagcgcgc accagcactg gttgcgcctt
     2701 tttcagatag tgcgaagacg gatgatccgc gtgccgctgg attggtaaac gcatctgcat
     2761 gaatggataa aaataagtcc gcttgcaact ttctggcctt gatcacgcgg tcatgcagtg
     2821 gaataaaata gtcgccatca cgcgttagaa tggcccgcat gttgggttca gcatcaattt
     2881 tttcttttag tttttttgca atcagcaaag ttacatcttt ttcacggcta ccactggcac
     2941 cgatagcacc tgggtcttca ccgccatgcc cagggtcaat cgcaattgtg atttgccgaa
     3001 atgctggact atcagcttta ttcacactgg gtttataaga atctgacttt gttgcatcag
     3061 gcggcacaat tgcaacattt ttattgtcaa tgttgtcggc actgggcatg tctgacgttg
     3121 tcacgtcagt gtcttttgtc gtttcaggca gtttgctctg ctcggctatc tgcgtggtat
     3181 cagtcgttgg cgcattttct gctatgggtt gagaatctgt tgtactcact aaatttggta
     3241 acgcctcggt tgagccttgc ggttcagaag atggctgcga cgcatccacg gggatattcg
     3301 cctgcgcatt ggtcgcagca tcgcgcgctg ccagcatcgc cattaacggg tcatgcaagg
     3361 ggtgaatatc caacaccaag cgatggttgt aatcgccagt aggcttcaag gaaaatacat
     3421 taggcttgga aacgtctttt aaatcaacca ctaagcggac tacattttgt tgaaaacgcg
     3481 ccacacggat ttgcttgatg tacgggtctg tcggtagaac gcgatcattg gtggatttaa
     3541 tgatttgatt gaattcaaca ttttcaatat ccagcacaac acgatcaggg tttgaaataa
     3601 tctgcatctt gtaaacaaat ggcttgttag attcaagcgt aatgcgggta aaatcctgcg
     3661 cgggccaaac acgtgacgca ataattttat tttctgcgtg tgcgatattg gccattgcca
     3721 gcgtcaagca aagcatggcg tacacgctaa agcgcatcaa cgcctgcatt atggcttgcc
     3781 ttgtaaatga atagcttgtg aaatgatggc ttgtaaacat tgttgtccta atacggaatg
     3841 cgcaatgatt tgtatttttc gcccgatgga ctgtattgaa aatacaatat caatatccgg
     3901 ggttggcaat acctgctgcg ctttttctgg ccattcaatt atacacacgc tgcgtgcatt
     3961 aaaatagtct ctaaaacctg ctgcgttcca ttcttcttca tcattaaaac gatataagtc
     4021 aaaatgatac aagtgacatt tagagtttgg ttgaagttca atatcgtaca tttcaaccaa
     4081 ggtgtaggtt gggcttttta ctttgccagc aaagcccagc gcatgcataa cgccgcgcac
     4141 taaagtggtt tttcctgcgc ctaaatcgcc atgcaaataa atgacgaaat ttggcaccag
     4201 cgctttggct aaaatcgcgg caaactgcaa cgtcgctgct tcatcggcca aatcaagtgt
     4261 aaaatcatgc tccatgacga ctcaccctat ggatttcacg cagttgagtg cagcaattaa
     4321 gcgctgggga atagccttgg gctttagtga tataggcatc accgacacgc atctgcagac
     4381 agcagaaacc gaacaccaag tgtggattaa caaaggtttt catggtgaca tggattatat
     4441 ggcaaaacat ggcacgaagc gaacccgccc agcggaatta gtgcctaaca ccacacggat
     4501 tatttcggcg cggctcgatt atttaccgcc gcaatccgca gacagtgaag cgattttgca
     4561 agcaccaaat caagcattta tttcgcgcta tgcattggga cgcgattacc acaaagtgat
     4621 gcgaaaaaaa ctgcaaaaac tttgcgataa catacaagcg gcgctagtga atgatgcagc
     4681 cgatgctttt gaataccggg tattcaccga tagcgcacct gtgctagaag tagcaattgc
     4741 tgaaaaatcc ggcttaggct ggcgtggaaa acataccctt ttaattaaca aagaccgcgg
     4801 ttcatggttt tttttaggag aaatatatac caatttaccg ctcattattg acgcacctgc
     4861 cagcaatcac tgcggtactt gtacaagttg tattgaggtt tgccctacac aggccatcac
     4921 tgcgccctat gaagtggatg ccagacgctg catctcttat ttaaccattg agctcaaaac
     4981 gtccattcca atagaattca ggccgctcat cggcaaccgc atttatggct gcgatgattg
     5041 ccagttgttt tgcccctgga ataaatttgc acaaatcacg accgaagccg attttgccat
     5101 taaacacggt ttggatacga ttacactgct tgaatgtttt ttgtgggatg aatggacttt
     5161 taaagaaaaa atggccggca gtgcgattta tcgcattggc tacccacaat ggctacgcaa
     5221 cgtggcggtc ggcttgggca atgcaaacac aacacctgaa attatcacag cactaaaagc
     5281 gcgtgagttt gatgaaaatg cacttgttcg agagcatgtt gtttgggctt tgcaacagca
     5341 tttaaacaaa caataaattc gccgagaaaa tttttatgct ctggaagcct ttatttataa
     5401 ggcttccaga gcatcgaaaa aggttgttat ctaaaacagc ttatttttca ttgttgttaa
     5461 gacaaaaata tttaacccag attttccatt aggacttgaa gtggcaacgc cgcacagttt
     5521 gatggatttt
//
LOCUS       DAOQSI010000133         5523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_15094_length_5523_cov_5.328822, whole genome shotgun sequence.
ACCESSION   DAOQSI010000133 DAOQSI010000000
VERSION     DAOQSI010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15094_length_5523_cov_5.328822"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2101
                     /locus_tag="PKL42_08250"
     CDS             <1..2101
                     /locus_tag="PKL42_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNU67103.1"
                     /translation="LRTGVVVAVVIPVVLAATFLGMHWLNIGLHKISLGALILALGLL
                     VDDAIIAVEMMSSKMEQGWDRAKAASYAYTSTAMPMLTGTLVTVAGFLPIATAVSSTG
                     EYTRSIFQVSAIALIISWFAAVIFVPYLGYHLLPDYSKKPQPLKLTRWFKRKLGLKEA
                     NIAERETTETHHHDIYNTAFYRTFRKLVTNCVRYRKTVILITAAMFVLSVMGFSKVQQ
                     QFFPDSTRLELVVDLRLTEGASYHATNQEVKKLELWLKNWQKLHPSSENQGIDNYVAY
                     IGTGSPRYYLPMDIQLAHRGFAQFVILSKDLKTREALRTDLLHLFENDFPNLRASVMR
                     LENGPPVGFPVQFRVGGSDIPKIREISHKIAGIMRANPHLTNVQLGWEEPSKVMKVSI
                     DQSKARLLGVSSVDIANMLNGAMSELYITEFREGNERIDLVARGAEIERTKLSRLPNL
                     MINTQNGTSVPLSQIATISSEFEEGVIWRRNRVPSITVRANLRGDMQAPVVSVQIEEK
                     LGEITANLPLGISVETGGAVEESAKGGVSVAKGFPLFLVAVLTLLILQLKSFSRVFLV
                     LLTAPLGLIGVTVALLLFNKPFGFVAMLGTIALSGMIMRNSVILVDQIDQDKASGLPP
                     WQAVVESTIRRFRPIVLTAAAAILAMIPLTRSVFFGPMAVAIMGGLAIATILTVLFLP
                     ALYAAWFKVKIPDSITS"
     gene            2161..2850
                     /locus_tag="PKL42_08255"
     CDS             2161..2850
                     /locus_tag="PKL42_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HNU67104.1"
                     /translation="MHHQHLKTERLSMPSNIARFNMIEQQIRTWEVLDPAVLALLDVV
                     PREDFVAEAQRGLAFADIELPIGHEQSMLSPKLEARILQSLNVKKTDKVLLIGTGSGY
                     LTALFAKQAQHVVSVDNFPDLSKIAGKRLKQQGIDNVTLVVANAATGYSAAAPYDVIV
                     FTGSLHVYPVEAEKMLNVGGRMFAVVGELPIMQATLTQRAAEGAAHHETLFETCLPPL
                     INAPQSSKFEF"
     gene            2865..4262
                     /locus_tag="PKL42_08260"
     CDS             2865..4262
                     /locus_tag="PKL42_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HNU67105.1"
                     /translation="MYKLKHLTVAISLAFASITAPYSVFAAENAVASPANQGTEQSLL
                     AIYHQALTHDATLASALSANQAAQEIIEQGKALYRPTVNFNAEANALSTDIRYLDSNL
                     PDGRSKFETYKYGVDARQPIYRKQNLVQIEQSKTQVSLADKQLHLSQQALILRTTQAY
                     FEVLIAQDKIDLIIAQKAAIQSQLDQAKANFEVGTATITDVNEAQARYDLIVAQEIAA
                     INEHQIAKHAIQNITGELPQKLATVKADIKPSQLDQNMEKWLEVATENNLNIQIQQDA
                     VKLSEQEIERSKAGHLPTLDAVASYTDNYANGSPSVFGTGNELKTGAIGLQLQIPLYQ
                     GGATSSKVRQAVINKQKALDDVEIARRQTELDTQRAYLNLSTSIAQIKALEQALISSQ
                     SQVDSTQLGYEVGVRTSVDVLNAQQQLFSAKRDLLQARYNYLVNIIRLKTASGIVTEA
                     DLADINQQLVLNSPQ"
     gene            4265..4822
                     /locus_tag="PKL42_08265"
     CDS             4265..4822
                     /locus_tag="PKL42_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HNU67106.1"
                     /translation="MHNLLTAKSTFSQLVIVDMQARLATAMPLEAMQSVTKNAAILAQ
                     AATLLTVPTLVTEQYPKGLGNTIAELSAFLPNIKPVDKLCFSCMAEAWFSRQLTADRP
                     QMVLAGMETHVCVLQTALDMLRVGKQVFVVEDAVISRNAANKANAIARLRDAGCIITN
                     TESVVFEWLGVASGEHFKAISQLIR"
     gene            4834..5424
                     /locus_tag="PKL42_08270"
     CDS             4834..5424
                     /locus_tag="PKL42_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67107.1"
                     /translation="MNWKVLLFIGVIGFGAFHHFQQRAVIHGPGVIAPNTPSQNTARQ
                     ADIQMNGFSLTPLELYEIEARVLSREDYTFGTEAELSPTDLAVGWGPMSDEAILKKIN
                     ISQGNRFYYWRVDSFPIPQRQIEIHSANMHIIPANDSVKRQLSKVRQGQLVKIKGLLV
                     EAKRADGWNWRSSLSREDTGNGACELMYVTDFYIRQ"
BASE COUNT         1548 a         1234 c         1281 g         1460 t
ORIGIN      
        1 gttgcgcacg ggtgtggtgg tcgcggtggt gattcccgtg gtgctggccg caacgttttt
       61 aggcatgcac tggcttaata ttggcctgca taaaatttct ttaggcgcac ttatcttagc
      121 actcggtctg ctagtggatg acgccattat tgccgttgaa atgatgtcat ctaaaatgga
      181 gcaaggttgg gatagagcca aagcggcctc gtacgcctac acctcaaccg ccatgcccat
      241 gctcactggt acactggtta ccgttgcagg gtttttaccc attgccaccg ccgtctcctc
      301 tactggggaa tatacacgct ccatctttca ggtttctgcg attgcgttaa tcatctcttg
      361 gtttgctgcg gtgatttttg tgccttattt aggttatcac ctgctgcctg attattcaaa
      421 aaaaccacag cctttaaaat tgacccgctg gtttaaacgt aaacttgggt taaaagaagc
      481 aaatatagcc gagcgtgaga ccactgaaac tcatcatcac gatatttata acacggcttt
      541 ttatcgtaca tttcgaaaac tcgtgaccaa ttgcgtgcgc tatcgtaaaa ccgtgatttt
      601 gattacagcg gcgatgtttg tactctcagt gatgggcttt agtaaagtac agcagcaatt
      661 tttccctgac tcaacacgcc tagagctggt ggtagatttg cgcctcacag agggtgcgtc
      721 ttaccatgcg acaaatcaag aggtcaaaaa actggagtta tggctaaaaa actggcagaa
      781 acttcaccca agctcagaaa accaaggcat tgataattac gtggcttata ttggtactgg
      841 tagcccgcgc tactatctgc ccatggacat tcaacttgca catcgcggct ttgcgcagtt
      901 tgtgatttta agcaaagact taaagacgcg tgaagcgcta cgcacagact tgctgcattt
      961 atttgaaaat gactttccaa acctacgcgc atccgtcatg cggttagaaa acggcccgcc
     1021 ggttggattt cctgttcaat ttagagtggg cggtagcgat atccctaaaa ttagagaaat
     1081 ctcccacaaa attgcaggca tcatgcgcgc aaacccacat ttaaccaacg tacaattagg
     1141 ctgggaagag ccgagcaagg tcatgaaagt ctccattgat caatcaaaag cgcggctatt
     1201 gggtgttagc tcagttgata ttgccaatat gcttaatggt gcgatgagcg agttgtacat
     1261 caccgagttc agagagggca atgaacgtat tgacttagtc gcacgagggg ctgaaattga
     1321 gcgtaccaaa ttatcgcgcc taccaaattt aatgattaat acgcaaaacg gcacgagtgt
     1381 accgctgtct caaattgcga ccatatcgtc tgaatttgaa gaaggcgtga tttggcggcg
     1441 taaccgcgta ccttccatca ccgtgcgcgc aaatctgcgt ggtgatatgc aggcccccgt
     1501 cgtatcagtg caaattgaag aaaaattagg cgaaatcact gccaacttac cgcttggtat
     1561 ttctgttgaa acgggtgggg cagtcgaaga atcagctaaa ggtggcgtat cggtcgcaaa
     1621 aggctttccg ctatttttag tggcagtact caccttgctc attttgcaac ttaaaagttt
     1681 ttcgcgggtc ttcttggtgt tactcacggc gccgctgggt ttaattggtg tgacagttgc
     1741 cttgctactg tttaacaaac cttttggctt tgtcgccatg ttgggtacga ttgcgctgtc
     1801 tggcatgatt atgcgaaatt cagtcatctt ggtggatcaa attgaccaag acaaagcttc
     1861 aggcttgccg ccttggcaag cggtggtgga atcaaccatt cggcgcttta ggccgattgt
     1921 gctgactgcc gctgcagcca ttctagccat gataccgctt acacgcagtg tgttttttgg
     1981 gccaatggct gtggcgatta tgggtgggtt agcgatagca accattttga cggtgctatt
     2041 tctaccagct ttatacgctg cgtggtttaa agtaaaaata ccggattcaa taaccagtta
     2101 agttataatc atttcagacg taatagagat tcaagaagag ctaatatgac atcatcgacc
     2161 atgcaccatc aacaccttaa aactgaaaga ttaagtatgc ccagcaatat tgcacgtttc
     2221 aacatgatag aacagcaaat ccgcacttgg gaggtgttag acccggcggt actggcgctt
     2281 ttagatgttg tcccgcgtga agactttgtt gcggaggcgc aacgcggttt agcctttgcg
     2341 gatattgagc tgcctattgg gcatgagcaa agcatgttat cgccaaagct tgaagcacgc
     2401 attctgcaat cattaaacgt taaaaaaaca gataaagtat tattgattgg tacaggtagc
     2461 ggatatttaa cagcgttatt tgcaaagcaa gcgcagcatg tcgtttcggt tgataatttt
     2521 ccggacctct caaaaattgc aggtaagcgg ctaaagcaac aggggattga taatgtgact
     2581 ttagtggttg ctaatgcagc gacaggttat tcggctgcag caccctatga tgtcattgtg
     2641 tttacgggtt ctctacacgt ataccctgtt gaagctgaaa aaatgcttaa tgttggcggg
     2701 cgcatgtttg ctgtggttgg cgagttgcca attatgcaag ccacactcac acaacgcgct
     2761 gcagaaggtg ctgctcatca cgaaacgtta tttgaaacgt gcttgccacc gctgattaat
     2821 gcaccgcaat cttctaaatt tgagttttaa ttcgaattaa tcctatgtac aaattaaaac
     2881 acctgacagt tgcaatttca ctagcctttg ccagcatcac agcaccttat agtgtgtttg
     2941 ccgctgaaaa tgcagtggca agccctgcga atcaaggtac agagcagtca ctgttagcta
     3001 tttatcatca agctttaaca cacgatgcaa cattggcatc tgcattaagt gctaatcagg
     3061 ccgcccaaga aattatcgag caaggtaaag cgctttaccg cccaaccgtc aactttaatg
     3121 ctgaagccaa tgccttatcc actgatattc gctatcttga ctcaaatcta ccggatgggc
     3181 gctccaagtt tgagacttat aaatatggtg tagatgctcg ccagccaatt taccgtaaac
     3241 aaaatttggt gcaaattgag caaagtaaaa cccaggtaag tcttgccgat aagcagctac
     3301 atctaagtca acaagcgcta atactacgca cgacacaagc ctattttgag gtgctgattg
     3361 cacaggataa aattgattta atcattgcac aaaaagctgc aattcaaagc cagctagacc
     3421 aagctaaagc caattttgag gtcggcactg ccaccatcac cgatgtcaat gaggcgcaag
     3481 cccgctatga tttaattgtt gcgcaagaga ttgccgcaat taacgaacac caaatcgcca
     3541 aacatgcgat acaaaatatt acgggcgaat taccgcaaaa attggcaacc gtaaaagccg
     3601 atattaaacc cagccaatta gaccaaaata tggaaaaatg gctggaagtt gcgacagaaa
     3661 ataatttaaa tattcagatt cagcaagatg ccgttaaact atcagagcaa gaaattgaac
     3721 gctcaaaggc gggccacctg cctacgctgg atgcagtagc gagttatact gacaactacg
     3781 ccaacggaag cccttctgtt tttggtactg gaaatgaact gaaaaccggc gcaattggtt
     3841 tgcaattgca aatcccgtta taccaaggcg gtgccacaag ctctaaagta aggcaggcgg
     3901 tcatcaataa gcaaaaggcg ctagatgatg ttgaaattgc ccggcgtcaa acggaactgg
     3961 atacacagcg cgcctattta aatttaagca cctcgattgc acaaataaaa gcgttagaac
     4021 aggccttgat tagtagccaa agtcaggtag attcaacaca actggggtat gaagttgggg
     4081 tgcggacgag cgttgatgta ctcaatgctc agcaacaact ctttagcgcc aagcgcgatt
     4141 tattacaggc gcgctacaac tacctcgtga atatcattcg cctaaaaaca gcctctggca
     4201 tcgtcactga agcagattta gctgatatta accagcaatt agtcctcaat agccctcaat
     4261 aataatgcac aacctactga ctgcaaaatc aacatttagc cagcttgtta ttgtggatat
     4321 gcaagcgcgg cttgcaactg caatgccgct agaggcgatg cagtcagtta caaaaaacgc
     4381 cgctattttg gcgcaggcag cgacattgct aacggtgcca acgctagtga ctgagcaata
     4441 ccctaaaggt ttaggtaata ccattgcaga actttctgca ttcttgccca acatcaagcc
     4501 agtagataaa ttatgttttt cttgcatggc agaagcctgg tttagccgcc agctgacggc
     4561 tgatcggcca cagatggtac tcgccggcat ggagacgcat gtttgcgtat tgcaaaccgc
     4621 gcttgatatg ctacgcgtag gtaagcaagt gtttgtagtg gaagatgcgg taatttcaag
     4681 aaacgccgca aataaagcca atgccattgc cagattgcgc gatgcaggtt gcatcattac
     4741 caacaccgaa tcagtggtat ttgagtggct aggcgttgct tcgggtgagc acttcaaagc
     4801 cattagtcaa ttaatacgtt aaagctaatc ggtatgaatt ggaaagtact ccttttcatc
     4861 ggcgtaattg gttttggggc ttttcatcat tttcaacaac gtgccgtcat ccatggtccc
     4921 ggtgtcattg cgccaaatac tccaagccaa aacacagccc gccaagcaga tatacaaatg
     4981 aacggctttt cactcacgcc gcttgaactc tatgagattg aagcaagagt gctttcgaga
     5041 gaagattata cgtttggtac ggaggctgaa ttatcaccca cagatttggc agttggttgg
     5101 gggccgatgt ctgacgaggc catactcaaa aagattaata tttctcaagg taatcgtttt
     5161 tattattggc gtgtagattc gtttccaatt ccgcaacggc aaattgaaat tcactctgcc
     5221 aacatgcaca ttatccctgc taacgacagc gttaaacgcc agttatccaa ggttcggcag
     5281 gggcagttag tcaaaatcaa agggctgttg gttgaggcga aacgtgccga tggatggaat
     5341 tggcgaagtt ctcttagccg tgaagataca ggcaatggcg cttgtgagtt gatgtatgtg
     5401 actgattttt atattcgcca ataaattagg ctacacgtat atattgctga atcaacttta
     5461 atgttagctc agtagcgccc tgtgacgctt gacataaagc aaggcctttt gcacccattt
     5521 cgg
//
LOCUS       DAOQSI010000134         5501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_15198_length_5501_cov_5.196107, whole genome shotgun sequence.
ACCESSION   DAOQSI010000134 DAOQSI010000000
VERSION     DAOQSI010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15198_length_5501_cov_5.196107"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /gene="pqqD"
                     /locus_tag="PKL42_08275"
     CDS             complement(<1..189)
                     /gene="pqqD"
                     /locus_tag="PKL42_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HNU67108.1"
                     /translation="MTQQSISKNDIYAIAMHHRFQWEEAQQCYVILFPEGMVKLNGGA
                     GEVLKRVNGKASVADIVAD"
     gene            complement(202..969)
                     /gene="pqqC"
                     /locus_tag="PKL42_08280"
     CDS             complement(202..969)
                     /gene="pqqC"
                     /locus_tag="PKL42_08280"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HNU67109.1"
                     /translation="MTNETQAPWSREEFEEKLREKGKGYHIYHPIHVLMYEGKLTQTQ
                     MQAWVANRYYYQIAIPMKDAAILSNCPDRELRRGWIQRIFDHDGSGNIAEGGEGGIEA
                     WIHLGHAVGLTREEVMSLKLVAPGTFFAVDAYINFAKQRPWQESVCSSLTELFAPHIH
                     QQRIDSWPDMYPWINPEGMQYFKNRLTQARRDVEQGLSITLDYFGRTRELQERALEIL
                     QFKLNVLWVMADSIMLQCADIKVEGRDYLRQPVIEIK"
     gene            complement(1113..2039)
                     /gene="pqqB"
                     /locus_tag="PKL42_08285"
     CDS             complement(1113..2039)
                     /gene="pqqB"
                     /locus_tag="PKL42_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HNU67110.1"
                     /translation="MHIHVLGAGAGGGFPQWNCNCFNCDGLRKGTIKATKRTQSSICV
                     SSNGVDWVLFNASPDVLQQIQDFAPLQPARAIRDSGIQAIVLIDAQIDHTTGLYMLRE
                     GQVKREIYCTEQVYGDLTSGNQILNILGHYCGINHHQVPIDGATSFTIPNVPNLKFTT
                     VALKSAAPPYSPHRNNPQAGDTIGVLIEEISTGKKCWYSPGLAEIEPHLPELMQQADC
                     IMVDGTFWTDTEMIDLGLMTKSARSIGHNPQSGENGMIEDLDKFGQDKKVRKVLIHIN
                     NTNPILREDSAERATLTQHGIEVAYDGMDIIL"
     gene            complement(2123..2194)
                     /gene="pqqA"
                     /locus_tag="PKL42_08290"
     CDS             complement(2123..2194)
                     /gene="pqqA"
                     /locus_tag="PKL42_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HNU67111.1"
                     /translation="MWTTPAATEMRFGFEVTMYVCNK"
     gene            complement(2502..3578)
                     /gene="aroG"
                     /locus_tag="PKL42_08295"
     CDS             complement(2502..3578)
                     /gene="aroG"
                     /locus_tag="PKL42_08295"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HNU67112.1"
                     /translation="MTTYHKLATDDVRIREIKPLISPISLLERLKESADATHNILNAR
                     AAIHRILHQLDDRLLVVVGPCSIHDTKAGMEYAHRLLTIKKELAADLMIVMRVYFEKP
                     RTTVGWKGLINDPHLDGTYQINEGLEIGRKFLLDVNEIGMPAGAEFLDTITPQYTADL
                     VSWGAIGARTTESQVHRELASGLSCPVGFKNGTDGGVRVAIDAIKTATSPHHFLSFTK
                     EGKAAIFSTAGNDDCHIILRGGKEPNYDAENVNEVCRQLTEAGLSPMLMIDCSHANSS
                     KDFSRQKIVAKNIAEQLSNGDQRIMGVMLESHLKEGRQDHAPGCDLVYGQSITDACLS
                     WEDTVDQLEMLAAAVRYRRQKTHQ"
     gene            complement(3595..4686)
                     /gene="hisC"
                     /locus_tag="PKL42_08300"
     CDS             complement(3595..4686)
                     /gene="hisC"
                     /locus_tag="PKL42_08300"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNU67113.1"
                     /translation="MKNLAPNYIQAIAPYLGGKPISELAREMGLNEADIVKLASNENP
                     LGISPKAEFAIQEALQDIARYPDGNSFILREAVSQKFNVAQNQIVFGNGSNDILELAA
                     RAYLTAGCEAIYSQHAFAVYPLVTQATGATGVVVPAKDFGHDLDAMLLAITPKTRMIF
                     VANPNNPTGTLLAKAALLDFLKRVPNNVLVVLDEAYDEYLSETHKSAAIGWLQQFENL
                     IISRTFSKAYGLAGLRIGFGLMHADVADMLNRVRQPFNVNSIAQAAAVASLADDDFVA
                     RSYALNQAGMTQLTQGFKKLGLEYIPSFANFVSFKVKDAALVNQKLLKNAVIVRPIAN
                     YEMPDYLRVSVGLFAENARFLAVLESILE"
     gene            complement(4814..>5501)
                     /locus_tag="PKL42_08305"
     CDS             complement(4814..>5501)
                     /locus_tag="PKL42_08305"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNU67114.1"
                     /translation="ILSFSALFALFSFSFNESLSYLIVLTSAFGISVFSSYIAEKSDR
                     ALFLREKSLDRERHEHRMRAMHDELTQLPNRALLIDRIEQVILDSRRNNQFCAGFFLD
                     LDNFKEVNDTYGHAVGDAVLNEVSQRLTATIRAADTVARLSGDEFFVLARDVKNKAQA
                     EAFGHKLLRKINKPYQIDGKVVSNSLSVSVGICLFPYVGVTPLDMIKKADHAMYQVKL
                     SGKSGIAFAG"
BASE COUNT         1518 a         1321 c         1108 g         1554 t
ORIGIN      
        1 atcagccaca atatcagcca cgctggcttt accgtttacc cgtttgagca cttcacccgc
       61 accaccgttc aatttcacca taccttctgg aaacaatatc acgtagcatt gttgcgcttc
      121 ttcccactga aagcggtggt gcattgcgat tgcataaatg tcattcttgg agatagattg
      181 ttgggtcata tttacctact tttatttaat ctcaatgact ggctgacgca aataatcacg
      241 tccttccacc ttaatatctg cacattgcaa catgattgaa tcggccatca cccacaacac
      301 atttaactta aattgcaaaa tttcaagcgc gcgctcttgc aactcacgcg tgcggccaaa
      361 ataatcaagc gtaatagata aaccctgctc aacatcgcgg cgcgcttggg taaggcgatt
      421 tttaaaatat tgcatacctt cggggttaat ccatggatac atatcaggcc aggaatcaat
      481 gcgctgctgg tgaatatgcg gcgcaaatag ttcagttaag ctagagcaaa cgctctcttg
      541 ccaagggcgt tgtttagcaa agttaatgta tgcatccact gcaaaaaaag taccaggcgc
      601 aactaatttg agtgacatca cctcttcacg cgttaatcca accgcatggc ctaaatgaat
      661 ccacgcttca ataccgccct cgccgccttc cgcaatattg ccgctgccgt catggtcgaa
      721 aatgcgctgt atccaaccac gacgtaattc acggtcagga cagtttgaaa gaatcgctgc
      781 atccttcatc ggaatggcga tttgatagta ataacggttg gccacccaag cctgcatttg
      841 tgtttgtgtt agtttgcctt catacatcag cacgtgaatt gggtggtaaa tatggtaacc
      901 cttacctttt tcacgcaatt tctcttcaaa ttcttcacga ctccaaggag cttgtgtttc
      961 attcgtcatt ttttatcctt ttattaatta aaaaaaatca aggggctaga tgagacaagg
     1021 cgtaagaaaa ttttttgagc gcggcatacg gggttgtatg taagcgataa aaatttttga
     1081 aacaacgcag catcatgacg caccttggtt ttttacaaaa ttatgtccat gccatcatag
     1141 gcaacttcaa ttccatgctg tgtcaacgtt gcacgctcgg cagaatcttc tcgtaaaata
     1201 gggttggtat tgttgatgtg aatcaatacc ttacgcactt ttttatcttg gccaaacttg
     1261 tctaagtctt ctatcatgcc gttttcaccc gattgcgggt tatggccaat gctgcgcgca
     1321 gattttgtca ttaaacctaa atcaatcatt tcggtatcag tccaaaatgt gccgtctacc
     1381 ataatgcagt cggcttgctg catcaactct ggcaagtgcg gttcaatttc agccaagcct
     1441 ggtgaatacc agcatttttt gcctgtgctg atttcttcaa tcaacacgcc aatggtatcg
     1501 cccgcttgcg gattattacg atgcggcgaa taaggcggcg cagcgctttt gagcgcaact
     1561 gtggtaaatt ttaaattggg cacattggga atggtaaagc tagtcgcccc atcaatcggc
     1621 acttggtgat gattgatgcc acaataatgg ccgagtatat tcaaaatctg gttacctgaa
     1681 gttaaatcgc catagacttg ctcggtgcaa taaatctcgc gtttcacttg cccttcacgc
     1741 aacatatata agcctgtggt gtggtcaatc tgcgcatcta tcagcacaat ggcctgaata
     1801 cccgaatcgc gtatcgcgcg tgcgggttgt agtggcgcaa aatcttgaat ttgctgcaac
     1861 acatcggggg atgcattaaa taacacccaa tccacaccat tactgctcac gcaaatcgaa
     1921 gattgcgtgc gcttggtggc tttaatggta cctttgcgca aaccatcgca gttaaaacaa
     1981 ttacaattcc actgagggaa accaccccct gcgccggcac ctaaaacatg aatatgcata
     2041 tcatcactca aaaaagatct atataaaaat actgcttaaa ttaaaaaggc taccttgtgg
     2101 tagccttttt tgcaccttgc aattatttat tgcaaacgta cattgttact tcaaaaccga
     2161 aacgcatttc agtagctgct ggagttgtcc acatgatatt tttcctttat taagttatta
     2221 agtcattcgc tgctgccttg atgatttaag tgctacaacg tggaacgaat tgtgcgcgtg
     2281 ctttaaactt tacaacaccg ctaacttacc taatatgcac tacgtaattt catgatgcaa
     2341 gacgctgttt aatcactaac ctttttcgat gccctgcgag ccttataaat aaaggcttgc
     2401 agggcaatgt ttttctctcg gggtattgtt atacaaattt caagcagttg tgcacaactt
     2461 tacttgcaat taaacccacc caatagctgc aaaaaatatg tttactgatg ggttttctgc
     2521 cgacgatatc ttactgctgc cgccaacatt tcaagctggt ccactgtatc ttcccagctt
     2581 aaacaagcat cggtaatcga ttgcccatag actaaatcgc aacctggcgc atggtcctga
     2641 cggccttctt tcagatgtga ttccaacatc acacccataa tgcgttggtc gccatttgaa
     2701 agttgttcag cgatattttt ggccacaatt ttttggcggc taaaatcttt actgctattg
     2761 gcatggctac agtcaatcat caacatgggt gacaggcctg cctcggtaag ttgccgacac
     2821 acttcgttga cattttccgc atcgtagttc ggctcttttc caccacgcaa aatgatatgg
     2881 cagtcgtcat tgccggcggt agaaaatatc gcggccttgc cttctttggt aaatgaaaga
     2941 aaatgatgcg ggctggtcgc agttttaatc gcatctatcg ccacgcgcac accaccatcg
     3001 gtaccatttt taaagcccac tgggcatgat aaccctgagg ccaattcacg atggacttgt
     3061 gactccgtcg tacgcgcacc aatcgcgccc caactgacta agtctgcggt atattgcggt
     3121 gtaatcgtat ctaaaaactc cgcgcctgcc ggcatgccaa tttcattaac atccagcaag
     3181 aatttgcggc ctatttctaa accctcatta atttgataag tgccgtctaa atgcgggtcg
     3241 ttaatcaagc ctttccaacc tactgttgta cgcggctttt caaaataaac gcgcatgaca
     3301 atcattaaat cagcagccag ctctttttta atagtcagca agcgatgagc gtattccatg
     3361 ccggccttgg tgtcatgaat tgaacaagga cctaccacga ctaatagtcg atcatcgagc
     3421 tgatgaagaa tgcggtgaat tgccgcgcgc gcattcaata tattatgggt tgcatcagcg
     3481 ctttctttta aacgttcgag cagtgagatg ggcgaaataa gcggtttaat ctcgcgaata
     3541 cgaacgtcat ccgtcgctag cttgtgatag gtggtcatgt ctgtgtgttt acttttactc
     3601 taaaatagat tctaacaccg ccaaaaagcg ggcattttca gcaaataagc ccacgcttac
     3661 gcgcagataa tctggcattt cataattggc aatcgggcgc acaattacgg catttttcaa
     3721 aagtttttga ttcaccaacg cggcgtcttt tactttaaag ctgacaaagt tggcaaacga
     3781 tggaatatat tccaagccaa gctttttaaa gccttgcgtt aattgtgtca taccagcttg
     3841 attcaatgcg tagctacgtg ccacaaaatc atcgtctgca agcgaggcca cggcggcggc
     3901 ctgcgcaata ctattcacat taaagggctg gcgtacacgg ttaagcatat cagccacatc
     3961 cgcatgcatc aggccaaaac caatgcgcaa accagctaaa ccatacgctt tagaaaatgt
     4021 gcgtgaaata attagatttt caaattgttg caaccaacca atcgccgcag atttatgcgt
     4081 ttcagacaga tactcgtcat aagcttcatc cagcaccact aacacgttgt taggcacacg
     4141 ctttaaaaag tccaaaagcg ctgctttcgc cagcaacgtg cccgttggat tattggggtt
     4201 ggccacaaaa atcatgcgtg tttttggcgt aattgcgagc agcattgcat ccaaatcatg
     4261 gccaaaatct ttagctggca ccacaacgcc ggtcgccccc gtggcctgtg tcactaaagg
     4321 gtaaacggca aacgcatgct gcgaatagat tgcctcgcaa cctgctgtta aataggctct
     4381 tgcagcaagc tctaaaatgt cgttcgagcc atttccgaac acaatttgat tttgtgcaac
     4441 attaaatttt tgactcaccg cctcacgcaa tataaaacta ttgccatccg gatagcgtgc
     4501 aatatcttgc aacgcttctt gaatggcaaa ttcagccttg gggctaatcc ctaatgggtt
     4561 ttcattagat gccagcttta caatatccgc ttcatttaag cccatctcac gcgcaagttc
     4621 tgaaattggc ttgccgccaa ggtatggcgc aatcgcttga atatagttag gggctaggtt
     4681 tttcatgata ttgtttacag aaagtaattt gccggttaaa gcattagtta aataaaaatg
     4741 cggctttgcg ccgcaagtca tgggtcattt taacatgtct ttcatgctaa caacatggcg
     4801 aaattttcat ctcttaacca gcgaatgcta tgccggactt tccactcaac ttcacttgat
     4861 acatcgcatg gtcggctttt ttgatcatat caagtggtgt aacacctaca tacggaaaaa
     4921 gacaaatgcc aacactcaca gacaatgagt tactcaccac cttgccatct atctggtatg
     4981 gtttattaat ttttcttaaa agcttatgcc cgaaagcttc cgcctgggct ttatttttaa
     5041 catctcgcgc caatacaaaa aattcgtccc ctgataaacg ggcaacggtg tcagctgctc
     5101 gaattgttgc agtcaggcgt tgactaactt cgttgagcac cgcatcgcct accgcatggc
     5161 cataagtgtc attcacttct ttaaagttat ccaaatccaa aaaaaagccc gcgcaaaatt
     5221 ggttgttcct tcttgaatct aaaatcacct gttcaatacg gtcaatgagc aacgctcgat
     5281 taggcaattg tgtgagttca tcatgcattg cacgcatacg gtgctcatgc cgttcgcgat
     5341 ctagggattt ttcccgcagg aataacgccc tgtcactttt ttcagcaatg taacttgaaa
     5401 aaactgaaat accaaaagcg gacgttagca caatcaaata actcagagac tcattgaagc
     5461 taaatgaaaa taaagcaaaa agcgcgctga aagacagtat g
//
LOCUS       DAOQSI010000135         5459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_15357_length_5459_cov_4.298853, whole genome shotgun sequence.
ACCESSION   DAOQSI010000135 DAOQSI010000000
VERSION     DAOQSI010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 5459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5459
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15357_length_5459_cov_4.298853"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PKL42_08310"
     CDS             complement(<1..427)
                     /locus_tag="PKL42_08310"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNU67115.1"
                     /translation="MTDEVKPDASDNQNVAPLGEVLLAARKAKKLTQKDISNSLKLSI
                     KQIDAIEKNEFSLLPDATNTRGFIRNYARLLNVDAEPLLASFRARVPEILPTTLSVQS
                     STRQVKLSKESQPWIKYILASILVLLFLLAWFFYMDYMPK"
     gene            complement(430..1203)
                     /gene="pilW"
                     /locus_tag="PKL42_08315"
     CDS             complement(430..1203)
                     /gene="pilW"
                     /locus_tag="PKL42_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HNU67116.1"
                     /translation="MNIRLSKQLVLYTVFSAMIIFGLNGCATQQEQAKEKENTKSRAR
                     AHTDLGAVYFQQKQLEVALEEFTIATQIDASYAAAYNGLGLVNAALGRDDVADTHYKK
                     AIQLEPNNSESRNNYGSFLCSRNRIDESIPQFLAAVKNPLYATPVIAYTNAGICALRK
                     KESTSGEAYLQKALELEPLSGVAAYHLANSQFKRKDALSAKKTIQNVMLAQPGPELLW
                     LAIQIERVLGAKDAEASYALQLRSMYPDSEQAKLLQNGK"
     gene            complement(1208..2308)
                     /gene="rlmN"
                     /locus_tag="PKL42_08320"
     CDS             complement(1208..2308)
                     /gene="rlmN"
                     /locus_tag="PKL42_08320"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008107478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNU67117.1"
                     /translation="MINLLNYNQTQLAEYFKSIGEKPFRAKQLTRWIHHFGVHDFAQM
                     TDIAKSLRDKLVQETEITLPIVQLEKISNDGTRKWLIGADVANSIETVFIPEDDRGTL
                     CVSSQIGCALACTFCSTGAQGFNRNLSVSEIIGQLAIANHSLRQEAGYDLLSANDRII
                     SNVVMMGMGEPLTNYDNVVTAMQIMLDDSAYGLSRRRVTLSTSGIVPAMDKLKEDCPV
                     ALAVSLHAPNDALRDVIVPINKKYPLKDLMAACNRYLEKAPRDFVTFEYVMLDGINDT
                     VEHAHQLLSLVKNVSCKFNLIPFNPFPNSGYETSKPSHIRVFRDILMQAGYVVTIRKT
                     RGDDIDAACGQLAGKVLDKTKRNAIRIPIEAV"
     gene            complement(2344..2748)
                     /gene="ndk"
                     /locus_tag="PKL42_08325"
     CDS             complement(2344..2748)
                     /gene="ndk"
                     /locus_tag="PKL42_08325"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNU67118.1"
                     /translation="MAVQRTLSIIKPDAVAKNVIGKIYTRFENAGLKIVAAKMAQLTQ
                     AEAEGFYAVHKERPFFADLVKFMISGPVMIQALEGENAVQVNRDLMGATNPKEAAAGT
                     IRADFAESIDANAVHGSDSAENAAIEIKYFFG"
     gene            3012..4265
                     /locus_tag="PKL42_08330"
     CDS             3012..4265
                     /locus_tag="PKL42_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNU67119.1"
                     /translation="MKVNSNIKIAVAAALFGVACQSQAAGFALIEQSASGMGNAYAGG
                     GAVAEDASTIFFNPAGMTYIEGTQIVGAIHLIKPNAEFNDNGSVKALFRPQGGDGGDA
                     GDLAFVPNFYYKRDLTDSVKFGLGINAPFGLKTEYNKDWIGRFQGIKSEVKSVNINPA
                     IAFKVNDQLSLGLGVSAMWFQAELTRAVNLGAAERSVKIKGDDWGFGVNLGAIYQATA
                     DTRLSLAYRSKVRQDLEGKSTSNYAPLNTDVTAGVSLPENVSFSAFSRVNDKWDVMGD
                     VTWTRWSRFKELRIDFANTLPDSVTAENWENTMRYSLGMNYHYSDALKLRAGVAYDQE
                     AIRDQYRTARIPGNDRKWLSLGANWKTSPNSAFDIGYSHLFISDAKIDDNQTVPFVAP
                     PGNGRLRGSYDGSVDILSLQYTHNF"
     gene            4540..>5459
                     /locus_tag="PKL42_08335"
     CDS             4540..>5459
                     /locus_tag="PKL42_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase"
                     /protein_id="HNU67120.1"
                     /translation="MDSAHLNATTMNATCPACGLLCDDVQIVIHQKTIQVRNQNCSKS
                     QRFFEQPQIVTTPKIAGKPASLQAAIQHAAQLLKAAKAPLFAGLSMDILGFRTLFNLA
                     QKTNAAMQHINAQSTQRNLRVLQSAGWQTTTLTEVKHRADVLVCFGTDIVTHNPRFFE
                     RFIWVKDALFCDANQRDIIYIGENINTSAGISPTGNKPTVLACNTEALPEVTAAFRAL
                     IQGKTLKCTHVAGIPISALSDIATKLKQAKYGVLAWVAKDLDFPHAELTIQNITESAV
                     LLNQTTRAASLALGGSDGDTSISYAHTWLSG"
BASE COUNT         1625 a         1196 c         1098 g         1540 t
ORIGIN      
        1 gcttaggcat gtagtccata taaaaaaacc atgccagtaa aaatagcaac accagaatac
       61 ttgctaatat atatttaatc cacggctggc tttctttgct taatttcacc tgacgagtcg
      121 aagactgaac gctcagggtg gtaggcaata tttctggaac acgtgctcga aaactagcca
      181 acaatggctc tgcatctaca ttcaataaac gtgcataatt acgaataaat cctcgcgtat
      241 tggtagcatc aggtaatagg ctaaattcat tcttttcaat ggcatcaatt tgtttaatgc
      301 ttagtttcag gctgtttgaa atatcttttt gcgtcagttt ttttgctttt ctggcggcta
      361 ataatacttc acctaacggc gcaacattct gattatctga agcatctggc ttaacttcat
      421 cggtcatgac tattttccat tctgcaataa cttcgcttgt tctgaatctg gatacatgct
      481 tcttaactgc aaggcataac ttgcttcagc atctttagca cctaacacac gttcaatctg
      541 aattgctaac caaagcaatt ctgggccagg ttgcgccagc atcacatttt ggatagtctt
      601 tttagcagat agcgcgtctt tgcgtttaaa ttgactatta gcgagatgat atgcggcaac
      661 accagataaa ggttccaact caagcgcctt ttgtaaataa gcttcaccac tcgtagactc
      721 ttttttacgt aatgcgcaaa tgcctgcatt ggtataagca atgacaggtg tcgcatataa
      781 ggggtttttt actgccgcca aaaactgagg aatggattca tcaattctat ttctggagca
      841 aagaaaactg ccgtaattat tgcgcgactc tgaattatta ggctcaagct gaatggcttt
      901 tttgtaatgc gtatcggcaa catcatctct accaagcgcc gcattcacaa ggccaagccc
      961 gttgtaggca gcggcataac tcgcatcaat ttgtgttgca atcgtaaatt cttctagcgc
     1021 aacttccaac tgtttttgtt gaaaataaac ggcgcctaaa tcagtatgcg cacgtgcgcg
     1081 cgatttagta ttttcttttt ctttggcttg ctcttgttgc gtggcgcagc cattcaagcc
     1141 aaaaataatc atggctgaaa acactgtata taacactaat tgcttgctca acctgatgtt
     1201 catatgctta aactgcctca atgggaattc tgattgcatt gcgttttgtt ttatctaaaa
     1261 ctttacccgc taactgcccg caagcagcat caatgtcatc gccacgggtt ttacgaatag
     1321 tcaccacata gcctgcctgc attaaaatat cacggaatac acgaatgtgg ctaggcttag
     1381 acgtttcata accactgttt ggaaaaggat taaacggaat aagattaaat ttacatgaaa
     1441 cgtttttaac gagactgagc aattgatgtg cgtgttcaac cgtgtcgtta atgccatcaa
     1501 gcatcacgta ttcaaaagta acaaaatcgc gaggcgcttt ttctaaatag cgattacaag
     1561 ccgccatcag gtctttgagt ggatactttt tattaattgg tacaatcaca tcgcgcaacg
     1621 catcattcgg tgcatgtaac gacaccgcca gtgcgaccgg gcaatcttct ttgagtttat
     1681 ccatcgctgg cacaataccg ctcgtcgata gcgtcacacg acggcgactt aatccataag
     1741 cactgtcatc taacataatc tgcatcgcag ttaccacgtt gtcgtagttg gtgagcggct
     1801 cgcccatacc catcatgaca acattgctga taatgcgatc attcgcactc aacaaatcat
     1861 agccagcctc ttgccgtagt gaatgattag caatcgctaa ctggccaata atttcagaca
     1921 cgcttaaatt gcggttaaac ccttgtgcgc ccgtgctgca aaaggtgcat gccagcgcac
     1981 aacctatttg agatgacacg cataatgtac cgcgatcatc ttcaggaatg aagaccgttt
     2041 cgatgctgtt ggcaacatcc gccccaatca gccactttcg ggtgccatcg ttagagattt
     2101 tttccaattg cacgatcggc aaagtaatct ctgtttcttg cactaattta tcacgtaagc
     2161 ttttagcaat atccgtcatt tgcgcaaaat catgcacgcc aaaatggtgt atccagcgtg
     2221 ttaactgctt agcgcgaaaa ggcttctcgc cgatactttt aaagtattcg gcgagttgcg
     2281 tttgattata atttaataaa ttaatcaatg tattattgag ttaatttaaa gtaaattatt
     2341 aaattaacca aagaagtatt taatttcaat tgcagcattt tcagctgaat ctgaaccatg
     2401 cacagcgttt gcatcaatgc tttcagcaaa atcagcacga atcgtaccag cagccgcttc
     2461 tttaggatta gttgcaccca tcaaatcacg gttcacttgc acagcatttt cgccttctag
     2521 cgcttgaatc atcaccgggc cagaaatcat aaatttaacc aaatcagcaa agaaaggacg
     2581 ctctttatgc acggcgtaaa aaccttcagc ctcagcttgt gttaattgcg ccatttttgc
     2641 agcaacaatt tttaagccag cattttcaaa acgtgtgtag attttaccaa tcacattttt
     2701 agctactgca tctggtttaa tgatagaaag cgtacgttga acagccataa caactccatt
     2761 taattaacat attgaaaaga acgataaaat accattttta gcacttttaa taaacctaaa
     2821 attgcattcc cctgataaat aaagtgtggt ctttttgaca caatttatat cgcttcatgc
     2881 cacttcagcg gcatctattg atgaaaactg tactatgcat ataaaaatca gtaagatgct
     2941 gtttgttaca atttatcacg acttgttaca agtacagtta aaacatcaat ctcaggcggg
     3001 ggagtttaaa catgaaggtc aacagcaaca ttaaaatagc agttgcagca gcactattcg
     3061 gcgttgcatg ccaatcacaa gctgcaggtt ttgcactgat tgagcaaagt gcaagcggca
     3121 tgggcaatgc ctacgcaggt ggcggtgctg tggctgaaga tgcaagtacg atatttttta
     3181 accctgctgg catgacatat attgaaggta ctcaaattgt cggggcaata cacttgatta
     3241 aaccgaatgc agaatttaat gataacgggt ctgttaaagc tctttttaga cctcaagggg
     3301 gtgatggtgg tgatgctgga gatttagcat ttgtaccaaa cttttactac aagcgagatt
     3361 taacagatag tgttaaattt ggccttggta tcaatgcacc atttggccta aagactgagt
     3421 ataacaaaga ttggattggc cgctttcaag gtataaaatc agaagtaaaa tctgtgaaca
     3481 ttaaccctgc aatagctttt aaagtgaatg accaactgtc tctaggttta ggcgtttctg
     3541 ccatgtggtt tcaagctgag cttacccgtg cagttaatct tggtgcagct gagagaagcg
     3601 taaaaatcaa aggtgacgat tggggatttg gggtaaatct tggtgcaatt taccaagcga
     3661 cagcagatac gcggttaagt ctagcttacc ggtcaaaagt taggcaagat ctcgaaggaa
     3721 aatcaacctc aaactatgct ccacttaaca cagacgttac agcaggtgtt tcactaccag
     3781 aaaacgtttc ctttagtgct tttagccgag ttaatgacaa atgggatgta atgggtgacg
     3841 ttacgtggac ccgttggagt cgatttaaag aactaagaat tgacttcgca aacacccttc
     3901 cagactcagt aactgctgaa aactgggaga acacaatgcg ctattctctt ggtatgaact
     3961 atcactatag cgatgctctt aaattacgtg caggggtagc ctatgaccaa gaagcaataa
     4021 gagaccaata tcgcactgca cgtattcccg gcaatgacag aaagtggctt tctttaggcg
     4081 caaactggaa aacatctcct aattcagcgt ttgacattgg ctactctcat ttatttatta
     4141 gcgatgcaaa aatagatgac aatcaaactg ttccctttgt ggctcctcct ggaaacggta
     4201 gattacgcgg tagttacgac ggcagtgtcg acatcttaag cttgcaatac acgcataact
     4261 tttaattaga ttgagcctca aaaaaccaat gggtttaagt gaagaactta aacccatttt
     4321 ttatgcgcaa aaataacctt aaaccagcta aaatttctcc gagaaaatat ttatgctctg
     4381 gaagccttat aaataaaggc tcgcagagca tcgaaaaagg ttgcggttaa atgaagccca
     4441 aaaaggcctt tttaatgtgg gcacataatg caccgttttc agcacaacaa ttgcaataaa
     4501 tattcacata aaacaaaagt ctgtggcaaa atctaattca tggattcagc acatttaaat
     4561 gcaaccacga tgaacgccac ctgccctgct tgtggtttac tttgtgatga cgtgcagatt
     4621 gtaattcatc agaaaaccat tcaggtgcgc aatcaaaact gtagtaaaag tcagcgcttc
     4681 tttgagcaac cgcaaattgt cactacccca aaaatagccg gcaaacctgc gagcctacag
     4741 gccgccattc agcatgctgc acaactgctc aaagctgcaa aagcgccatt atttgcgggc
     4801 ctaagcatgg atattcttgg ctttagaacg ctttttaact tggcgcaaaa aaccaacgct
     4861 gctatgcagc acatcaatgc gcaaagcacg caacgcaatt tacgtgtgct gcaaagcgct
     4921 ggttggcaaa caaccacatt aactgaagtt aaacaccgcg ccgatgtgct ggtgtgtttt
     4981 ggtaccgata ttgtcacgca taacccacgc ttttttgaac gctttatttg ggtgaaagat
     5041 gcgctttttt gcgatgccaa tcagcgtgac attatttata ttggtgaaaa tatcaacact
     5101 agcgcaggca tttcacccac gggtaacaag cctaccgtat tggcctgcaa cacagaagct
     5161 ttgccagaag tgaccgctgc attccgcgct ttaattcaag gtaaaacgct caaatgtacg
     5221 catgtagcgg gtatacccat cagtgcatta agtgacattg caactaaact aaaacaggca
     5281 aaatatggcg tattggcttg ggttgccaaa gatttggatt ttccacatgc ggagctcact
     5341 atacaaaaca tcaccgaatc ggccgtgctg ttaaaccaaa ccacgcgcgc tgcaagttta
     5401 gcgctgggcg gcagtgatgg cgacaccagc atcagctacg cgcatacctg gctaagtgg
//
LOCUS       DAOQSI010000136         5439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_15432_length_5439_cov_4.818722, whole genome shotgun sequence.
ACCESSION   DAOQSI010000136 DAOQSI010000000
VERSION     DAOQSI010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5439
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15432_length_5439_cov_4.818722"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..1278)
                     /locus_tag="PKL42_08340"
     CDS             complement(7..1278)
                     /locus_tag="PKL42_08340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HNU67121.1"
                     /translation="MAHIVIMGAGVGGMPAAYELKAKVGNKHQITVVNTVDYFQFVPS
                     NPWLAVGWRKRDKITFPIRPYLEKKGINFIAEAVTKIDAEQNTLQLANGDNLNYDYLV
                     IATGPKLSFEEVPGSGPNGGHTHSICSVDHAEKTWDAYQEFLKDPGPVIIGAMPGASC
                     FGPAYEFTFIMNTDLRKKKIRNKVPITYITSEPYIGHLGLGGVGDSKGMLQSEYRNND
                     VKWITNAKVTKVEAGKMFVSEFDKAGNIVKEHELPFKFSMMLPAFKGVDPVAAVEGLC
                     NPRGFVLIDEHQRSKKYNNIFAAGVCVAIPPVEVTPVPTGAPKTGYMIESMVTALVHN
                     IVADLDGQIADAKGTWNAICLADMGSTGAAFVALPQIPPRNVNWIKKGKWVHLAKIAF
                     EKYFIYKMKKGSSEPIYEKYVLKAMGIEKLD"
     gene            complement(1359..1952)
                     /locus_tag="PKL42_08345"
     CDS             complement(1359..1952)
                     /locus_tag="PKL42_08345"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNU67122.1"
                     /translation="MNNIILNSALLLIALTVPACATKINDEVATAKYLEESRNTALEF
                     RKKLGDVQKAQLESVGAENAIPICKVIAPAMAAEYSKNGQMLKRVSLKARNKTQGTPD
                     TWEKEVLESFDKAQLAGKPVDVMEASTVSKDADGRWFRYMKAIPTQPQCLQCHGKPNE
                     ISAAMKALLAREYPEDMATGYSVGEVRGAISIRRKVD"
     gene            complement(1962..2369)
                     /locus_tag="PKL42_08350"
     CDS             complement(1962..2369)
                     /locus_tag="PKL42_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNU67123.1"
                     /translation="MTNQNFFKSVSMVAASTALLLAGCQPLNIAAMTPNEAAKMFSEQ
                     KAIIVDVREDDEWKAQHIAGAIHIPLAQVESRLGELTQYKDSTVIMQCRSGKRSAKAA
                     STLQAAGFTKVYNLTGGIIAWDKDGLTTTKSNL"
     gene            complement(2393..3784)
                     /locus_tag="PKL42_08355"
     CDS             complement(2393..3784)
                     /locus_tag="PKL42_08355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HNU67124.1"
                     /translation="MWPKNTAKASVLLLALMTNQAHAFDLFEAWQAARVHDPQFASAR
                     AGAEAGRAKTLQAQALKSPQVSLNAGMAGVNSDNKISNAQFSNAQFAGGGAGTSGTTD
                     FRTRTDAGLNLNLNLRAEYPLYDAARNGSAMQLNKQAQIAEVQLTLNEQQLMQRVAQA
                     YFDTLMSAHTLSTLKSQLVAVTEALASAQARFKEGDVAVIDTHETQASYDLLASQVFE
                     AESNLQLSRAALADITGNLDAALVSLPQNAKLPMAISGDLQSWLARAKSNHPSIHAQQ
                     LQEEIAHAESGKFQAKYSPVVNLVALAGGDHLQGLGAHDADVSNRQISLGVQLSIPLY
                     SGGMREARYQEALALEDMTRNQTEFVRQQATREARTAWLAVTTGQGQVKALEQAERSA
                     EIKLDSTRLGREVGDRTTLDALNAEQELFNARLALYRSRYQTLLALLNLSAAAGELSE
                     PRLQEINLLLTAK"
     gene            complement(3799..4008)
                     /locus_tag="PKL42_08360"
     CDS             complement(3799..4008)
                     /locus_tag="PKL42_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNU67125.1"
                     /translation="MTSWKLVRIIAGLLILLSLALGVAESPFFVSQYWLYFTAFIGIN
                     LFQSGMTNWCLMENILRKLGVRAGC"
     gene            complement(4062..>5439)
                     /locus_tag="PKL42_08365"
     CDS             complement(4062..>5439)
                     /locus_tag="PKL42_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNU67126.1"
                     /translation="VPEVTNYQAYVGLAAPINFNGLVRQYYLRTGGEVGDIQVNLVDK
                     NHRSDKSHVIASRVRPELQKIAMKMSSPDKIVKVKVVEVPPGPPVISPIVAEIYGPDD
                     AERLSIARQVQHVFNSTEGIVDTDNSGIADAKKIELVVDKKKAALLGVSQAAIVSTLR
                     TGLAGEDVTYMHDASKYPAGTRLRLPVESQGDIEDLLKLSVSTSTGKLVPIRELVTIE
                     DTLREQPIYHKDLLPVNYVVGDMAGKLDSPLYGMFSMRDAVNKIQVPSGQLRETFISQ
                     PENNQRGYALKWDGEWQVTYETFRDMGAAYAVGLILIYLLVVAQFGSYLTPLIIMAPI
                     PLTIIGVMPGHALLGAQYTATSMIGMIALAGIIVRNSILLVDFINLEVATGKPFKEAV
                     INSAITRAQPIVLTALSAMLGAFFILDDPIFNGLAISLIFGIFVSTLLTLIVIPLLYF
                     VAYRHKFN"
BASE COUNT         1423 a         1334 c         1119 g         1563 t
ORIGIN      
        1 cacaatctaa tccagttttt ctatccccat tgccttaagc acatactttt catatatggg
       61 ctcagagctg ccttttttca ttttgtatat aaagtatttc tcgaaagcaa tcttagctaa
      121 atgtacccac tttccttttt taatccagtt tacattgcgt ggtggaattt gaggcaaggc
      181 tacaaatgcc gcgccagtgc tacccatatc agctaggcaa atcgcgttcc aagtgccttt
      241 tgcatctgct atttgcccat ctaagtctgc cacgatattg tgtacaagcg ctgtcaccat
      301 cgattcaatc atataaccag ttttaggcgc accagtaggt accggcgtaa cttctacagg
      361 cggaatagcg acacatacgc ctgcagcaaa aatattgtta tattttttgc tacgctgatg
      421 ttcatcaatt aatacgaaac ctcttgggtt gcataaacct tccaccgcag ccaccggatc
      481 aactccttta aaggcaggca gcatcattga aaatttgaac ggcaactcat gctctttcac
      541 aatattgcca gccttatcaa actcagacac aaacatttta cctgcctcga ctttggtgac
      601 cttggcattg gtaatccatt taacatcgtt gttgcgatat tcagactgaa gcatcccctt
      661 agagtcgcct acaccaccca atcccaagtg gccaatataa ggctcgctgg tgatataggt
      721 aataggcact ttattgcgga tttttttctt acgtaaatcc gtattcatga taaatgtaaa
      781 ttcataagca ggaccaaagc agctggcgcc tggcatggcg ccaataatga caggtcctgg
      841 gtctttaaga aactcctgat aggcatccca cgttttctca gcatgatcta cagaacagat
      901 ggaatgtgta tggccaccat ttggcccaga gcctggcact tcttcaaagg atagtttagg
      961 tccagtagca atcaccagat agtcgtagtt taaattgtca ccattagcaa gttgcaacgt
     1021 attttgttca gcatcaattt ttgtcacagc ctcagcaata aagttgattc cttttttttc
     1081 caaataaggg cgaatgggaa aagtgatttt atcgcgcttg cgccaaccta ccgcgagcca
     1141 tggattagaa ggtacaaatt gaaagtaatc caccgtattc accacagtaa tttggtgttt
     1201 attgccaacc tttgctttaa gctcgtacgc tgcaggcatt ccaccaaccc ctgcgcccat
     1261 gatgacgata tgtgccatgt tctaatccct taaatgaaaa tattagataa aatattcaat
     1321 tagtaaaatt ttattttttt gctgcatcca actaatgtct agtccacctt gcgcctaatt
     1381 gaaatggcgc cacgcacttc tccaacactg taaccagtcg ccatgtcttc aggatattcc
     1441 cttgccagca gtgctttcat agccgctgat atctcattgg gcttgccgtg gcattgcaaa
     1501 cattgtggct gggtcggaat cgctttcata tagcgaaacc agcgcccatc cgcatcttta
     1561 ctcacggtag aagcttccat gacatctaca ggcttgcctg caagttgcgc cttatcaaaa
     1621 ctctccaata cttctttttc ccaagtgtct ggcgtcccct gtgttttatt gcgcgctttt
     1681 aacgacactc gtttgagcat ttgcccattt ttggaatatt ccgcggccat tgcaggagca
     1741 atcactttgc atattggaat agcattctca gcacccacgc tttccagctg cgctttttgc
     1801 acatccccta gcttttttct aaattccagc gcagtgttac ggctttcctc tagatattta
     1861 gccgttgcaa cttcatcatt aattttagtg gcgcaggctg gcacagttag cgcgattaat
     1921 aacaaagccg aatttaagat aatattgttc atttaatgcg cctataagtt agattttgtt
     1981 gttgttagcc cgtctttatc ccaagcaata atgccgccag ttaagttata cactttggta
     2041 aaacctgccg cttgcagggt gctagccgcc tttgccgaac gcttgccgct gcggcattgc
     2101 ataatgaccg tgctgtcttt gtattgggtg agctcaccta gtctgctttc aacttgtgcc
     2161 aacggaatat gtatcgcacc ggcaatatgc tgcgccttcc attcatcgtc ttcacgcacg
     2221 tcaacaataa ttgctttttg ctcagagaac atttttgcgg cctcattagg cgtcattgca
     2281 gcaatattta aaggctggca acctgctaac aacaacgcag ttgaagccgc caccattgaa
     2341 acgcttttaa aaaagttttg attggtcaat tttttctatc cattcgtaat gtttatttag
     2401 cagtaagtaa tagattgatt tcttgcaagc gaggttcact taattcgcca gccgcagccg
     2461 aaaggtttaa tagtgccagc agcgtttgat aacggctgcg atagagtgca agccgtgcgt
     2521 tgaaaagctc ctgctcagca tttagcgcat caagtgtagt gcggtcgcct acttcacgcc
     2581 ctagacgggt ggaatctagt ttgatttctg cagaacgctc tgcttgctct aaagccttaa
     2641 cttgtccttg accagtagtg actgcgagcc atgccgttct tgcttcacgg gtagcctgct
     2701 gacgtacgaa ttcagtttga ttgcgagtca tgtcctcaag tgccagtgct tcttgataac
     2761 gggcttcgcg catgccacca ctataaagtg gaatagacaa ctgcacacca agacttattt
     2821 gacgattgct cacgtcggca tcatgagcac ccaagccttg caaatggtca ccacccgcta
     2881 gtgccaccag attaacaaca ggggaatatt ttgcttgaaa tttcccactt tcagcgtgtg
     2941 ctatttcttc ttgcagttgc tgtgcatgta ttgatggatg attgcttttg gcgcgcgcca
     3001 gccagctttg caaatcgcca ctaatggcca ttggtaattt cgcattttgt ggtagcgaca
     3061 ctaatgcagc atccagattg ccagtaatat cagccaaagc tgcacgagaa agctgtaaat
     3121 tgctctccgc ctcaaacacc tgcgaagcaa gtaaatcata gcttgcctgt gtttcatggg
     3181 tatcaatcac cgcaacatca ccctctttaa agcgagcctg agcgcttgcc agcgcctcgg
     3241 taaccgctac cagttgagac ttaagagttg atagcgtatg tgccgacatc aaggtatcaa
     3301 aataggcctg tgccacacgt tgcattagtt gctgttcatt cagcgttaat tgtacttcgg
     3361 caatttgcgc ctgcttgttg agttgcatgg ccgagccatt acgtgccgca tcatacaacg
     3421 gatattctgc gcgaagattc aagttcaggt tcaggcctgc atcggttctt gtgcgaaaat
     3481 ccgttgtgcc actagtccct gcgccgccac ctgcgaattg tgcatttgaa aactgtgcat
     3541 tactgatttt attgtcgcta ttgacgcctg ccatgcctgc attaagcgat acttgcgggc
     3601 tttttaatgc ctgtgcctgc aaagttttgg ctcgacccgc ttcggcacct gcacgcgctg
     3661 aagcaaattg cggatcgtga acgcgagcag cctgccaagc ctcaaaaagg tcaaaagcat
     3721 gcgcttgatt tgtcatcaat gccagcagta gtacagaagc ttttgcggta ttttttggcc
     3781 acatgatgtg ataaaccatt agcagcctgc acgtacgcca agtttacgta agatgttttc
     3841 cattaagcac caattagtca tgccactttg gaaaagattg atgccgataa acgctgtgaa
     3901 atacagccaa tattgactga caaagaatgg gctttctgcc acaccgagtg cgagcgacaa
     3961 taaaatcaac aagccagcaa tgatacgaac gagtttccat gaagtcatac ttacttctcc
     4021 tttagttttt aattaaatta attgttttaa ttaaattgtt tttagttaaa tttatgccga
     4081 taagccacaa aatacagcag tggtatcaca atcaacgtta gcaaggttga aacaaaaatg
     4141 ccgaatatta gtgaaatcgc taagccgtta aatattgggt catccaaaat aaagaaagcg
     4201 cccaacatgg cagataatgc cgtcagaacg ataggttgcg cacgggtaat ggcagagttg
     4261 atgaccgctt ccttaaacgg tttgcctgtg gcaacttcta ggttgatgaa atcaaccagt
     4321 aaaatcgagt tccgtacaat aatccccgcc agcgcaatca tgcctatcat agacgtggcc
     4381 gtgtattgtg cgcccagcaa cgcatggccc ggcatgaccc caatgatggt gagcggaata
     4441 ggcgccatga taatcaatgg ggttaaatat gagccaaact gagcaaccac cagcagataa
     4501 atcaatatca aaccaactgc ataagcggcc cccatgtcac ggaaagtttc ataggtaact
     4561 tgccattcac catcccactt caacgcgtag ccgcgttggt tattctcagg ttgcgaaata
     4621 aaggtttcac gcaactgacc acttggcact tgaattttgt tgacagcatc acgcatgcta
     4681 aacatgccat aaagcgggct atctaacttg ccagccatgt ctccaaccac gtaattcact
     4741 ggcaataaat ctttgtgata gataggttgc tcgcgcaaag tatcttctat tgtcaccagt
     4801 tctcgtattg gcaccagctt accagttgaa gtgcttaccg atagcttcag taaatcttca
     4861 atatcgcctt gtgattcaac aggcaagcgt aaccgcgtac ctgctggata cttgctggcg
     4921 tcgtgcatat aggtcacatc ttcacctgcc aaacctgtgc gcaaagtgct aacgatggcc
     4981 gcttgtgaca cgcctaataa tgccgctttt ttcttatcca ccaccagttc aatttttttc
     5041 gcatccgcaa tgccgctatt gtctgtatct acaatgcctt ctgtgctgtt aaacacatgt
     5101 tgaacttgtc tggcaatact taacctttcg gcatcatcgg ggccataaat ctcagccacg
     5161 ataggtgaaa ttacaggagg gcctggcggt acttcaacga ctttaacttt aacgattttg
     5221 tcagggctac tcattttcat cgcaattttt tgcagttccg gacgaacacg gctagcaatc
     5281 acatgactct tgtcgctacg gtgattttta tcaaccaagt tcacctgaat atcaccaact
     5341 tcaccaccag tgcgaaggta atattggcgc acaaggccat taaaattgat aggtgctgct
     5401 aggccaacat atgcttgata attagttact tctggcacg
//
LOCUS       DAOQSI010000137         5404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_15601_length_5404_cov_5.171621, whole genome shotgun sequence.
ACCESSION   DAOQSI010000137 DAOQSI010000000
VERSION     DAOQSI010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15601_length_5404_cov_5.171621"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..766
                     /gene="fae"
                     /locus_tag="PKL42_08370"
     CDS             194..766
                     /gene="fae"
                     /locus_tag="PKL42_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HNU67127.1"
                     /translation="MAVIDRVLIGEALVIENRPDGSGLDLKNVAHIDLIMGPRGSAAE
                     DAFCRTLTDQKEGVNGLLAIVAPNMMVKPNTVMFNKVTLRNTRQSVQMFGPAQRGVSM
                     AVMDCVADGTIPVEEADDVFICVGVFIDQSADIDDRIQDWNYQATKQAIKSAIAREPK
                     AAELLKVYKESAHPFAAHPEKRRASDKLAK"
     gene            861..1742
                     /locus_tag="PKL42_08375"
     CDS             861..1742
                     /locus_tag="PKL42_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNU67128.1"
                     /translation="MAKHEAQDDGREFDFTAQDFARVRKLIYQHAGISLSEAKTDMVY
                     SRVGRRLRIMGFTTFKDYLDNLENENSADEWEAFTNALTTNLTSFFREEHHFPILAEH
                     LQSVKTPIRIWCSAASTGEEPYTIAMTACEAFGSMRPPVEIIATDIDTNVLATASRGV
                     YPYERVSRLSLERRKQFFQKGSGKHEGMVRVRNELKSLITFHQLNLLEQQWPLKETFD
                     VIFCRNVMIYFDKPTQAKILSRFVPLMKPDALLFAGHSENFLYVSDAFHLRGKTVYDL
                     KQHAGKSATNRLAEAAL"
     gene            1739..2347
                     /gene="cheD"
                     /locus_tag="PKL42_08380"
     CDS             1739..2347
                     /gene="cheD"
                     /locus_tag="PKL42_08380"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemoreceptor glutamine deamidase CheD"
                     /protein_id="HNU67129.1"
                     /translation="MSMLEEEISTTLYYDRTFDCNAAKISPGEYYFTDKDMVIVTVLG
                     SCVSACIRDNISGIGGMNHFMLPDSAAADKDSPVSESMRYGTYAMEVLINQLLRNGAR
                     RENLEAKIFGGGNVLKSFTTMNVGDRNAMFVRKFLKEERIRVTGEDLLDIYPRKVYYF
                     PKTGKVLVKKLKNMHNDTLYKREEAYASKLKTSDVGGEIDLF"
     gene            2467..3531
                     /locus_tag="PKL42_08385"
     CDS             2467..3531
                     /locus_tag="PKL42_08385"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HNU67130.1"
                     /translation="MKIKVLVIDDSALIRSLLTEIINSQKDMEVVGAAPDPLIARDMI
                     KQLNPDVLTLDVEMPKMDGLDFLERLMRLRPMPVVMVSTLTERGSEITMRALELGATD
                     FVTKPKMSITEGMREYTEEITDKIRVASKARVQTLSRQPVAQNNTPAPLRNPLISSEK
                     LLIIGASTGGTEAIKAFLLQMPSDCPGIVITQHMPAGFTKSFANRLDSLCKISVKEAQ
                     GGERILPGHAYIAPGDKHLLLARSGANYVTQLSDEPPVNRHKPSVDVLFDSAAALAGK
                     NAVGVILTGMGKDGAAGMAKMKQAGAYNFAQNEESCVVYGMPREAVAHGGVHEVAHLQ
                     ELPKLVLNYLTANSARALRV"
     gene            3672..4070
                     /gene="cheY"
                     /locus_tag="PKL42_08390"
     CDS             3672..4070
                     /gene="cheY"
                     /locus_tag="PKL42_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HNU67131.1"
                     /translation="MANPNTKFLVVDDFSTMRRIVRNLLKELGYNNVDEAEDGVVALS
                     KLKGGGFDFVVSDWNMPNMDGLTLLQHLRADPELKHLPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLDEKLSKIFEKLEKAAA"
     gene            4070..4738
                     /gene="cheZ"
                     /locus_tag="PKL42_08395"
     CDS             4070..4738
                     /gene="cheZ"
                     /locus_tag="PKL42_08395"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HNU67132.1"
                     /translation="MITSETLQEPATLVSHAQALTPQDEMLSRIGHVTRTLHDSLRGL
                     GLDKIMEQVAQEIPDARDRLKYVAKMTEQAAERVLNATDAAMPMQDEILSQGNALETR
                     WKAILANASLKSEYNEIANETLTYLAATGKNSTETKALLMDIMMAQDFQDLTGQVIKK
                     ITVLAQELEQQLVQVLVDFSPGMVKKETENQLMNGPQIDPENTADVVASQEQVDDLLD
                     SLGF"
     gene            complement(4873..5373)
                     /locus_tag="PKL42_08400"
     CDS             complement(4873..5373)
                     /locus_tag="PKL42_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNU67133.1"
                     /translation="MTTLKYLQHYPPHLQKKILQLQAENKLGDYIAKRYPNAHAIQTD
                     KALYEYCHEIKQAYLRTAPPLNKVHYDSKLSIEHHALGLNTAISRVQGGKLKAKKEIR
                     ISAFFKAAPTEFLRMIVVHELAHLKERNHDKSFYQLCQYMEPDYHQFEFDCRVYLTWK
                     SQLANT"
BASE COUNT         1603 a         1085 c         1270 g         1446 t
ORIGIN      
        1 ccttttgaga ggctctagaa gccatgtaaa taaaggctca cagagcagta attttctctc
       61 gagcgtttta gtggttgttt ttgttatttt gtagtgctgt aaatagcgta atttttatta
      121 ttttttaata gatgcactgc acattcttaa aagaaaatgc gcatgtccat tctaataata
      181 taaggagtac aacatggctg taattgatcg agttttaatt ggtgaagcgt tagtgattga
      241 aaatcgtcca gatggaagtg gacttgattt aaaaaatgtc gcgcatattg acctgattat
      301 ggggccacgc ggcagcgcgg ctgaagatgc gttttgtaga acgctcaccg accaaaaaga
      361 gggggtgaat ggtttattgg caattgtggc accaaacatg atggttaaac caaacaccgt
      421 gatgttcaac aaagtaacgc taagaaacac aaggcaatct gtacaaatgt ttggcccggc
      481 acaacgcggt gtatcaatgg cagtgatgga ttgtgtggct gatggcacaa ttccggttga
      541 agaagcagat gatgttttta tttgtgtggg tgtatttatt gaccaatctg cagatataga
      601 tgaccgcatt caagattgga actaccaagc gaccaagcaa gcgattaaaa gtgcaattgc
      661 acgcgagcca aaagcagcag aattactgaa agtttataaa gagtctgcgc acccatttgc
      721 cgcacaccca gaaaaaagaa gagctagcga taagttggca aaataaaaag cagcatcact
      781 aacgtgcagg ttgaaatgtg caatctgcgc gcggcactgt aagcgatgta agtcagtgaa
      841 ggtttaagaa taaggtcaat atggcaaaac atgaagcaca agatgatgga agagagtttg
      901 attttaccgc gcaagatttt gccagagtgc gtaagctgat ttatcagcac gcgggcattt
      961 cactttctga agcaaaaact gacatggttt acagccgcgt tgggcgtcgg ctacggatta
     1021 tggggtttac tacctttaaa gactatctcg ataatcttga aaatgaaaac agtgccgatg
     1081 aatgggaagc ttttaccaat gcactgacga ccaatttgac ttcatttttc agggaagagc
     1141 atcattttcc gattttggca gagcatttac agtcagtcaa aacgcccatt cgaatctggt
     1201 gttcagccgc gtctaccggt gaagaacctt acacgattgc catgacggcg tgtgaagcgt
     1261 ttggcagcat gcgcccgcca gttgaaatta tcgccacgga tattgatact aacgtattag
     1321 ccacggcctc gcgtggcgtg tacccgtatg aacgtgtgag tcggctttca ttagagcggc
     1381 gtaaacagtt ctttcaaaaa ggctcaggca aacatgaagg catggtgcgc gtgcgcaatg
     1441 aactcaaaag cctgattacc tttcatcaac tcaatttact tgagcaacaa tggccgttaa
     1501 aagagacttt tgatgtcata ttttgcagaa atgtcatgat ttattttgac aaacccacgc
     1561 aagccaaaat tttaagccgt tttgtgccgt tgatgaagcc cgatgccttg ttgtttgctg
     1621 ggcattctga aaactttctc tatgtgtcag atgcttttca tctgcgtggt aaaaccgtgt
     1681 atgacctgaa gcagcatgca ggcaaatctg ccaccaatcg actagcggag gctgccctat
     1741 gagcatgcta gaagaagaaa tttcaaccac gttgtattac gaccgaacgt ttgattgcaa
     1801 tgcggccaaa atttcaccgg gcgaatatta ttttactgac aaagacatgg tcattgtcac
     1861 ggtgctgggt tcatgcgtat cggcctgcat tcgcgataat atttctggca tcggcggcat
     1921 gaatcacttt atgttgcctg atagtgcggc ggccgataaa gatagcccgg tttcagaatc
     1981 catgcgctac ggcacctatg cgatggaagt gctgattaac caattgctac gtaacggtgc
     2041 ccgccgtgaa aacctagagg ccaaaatttt tggcggtggc aatgtgctta aaagttttac
     2101 taccatgaac gtgggcgacc gcaacgccat gtttgtgcgt aaatttttaa aagaagagcg
     2161 tattcgtgtt acaggtgaag atttgctcga tatttaccct agaaaagttt attactttcc
     2221 aaaaacaggc aaagtgttag tcaagaaact aaaaaatatg cataacgata cactctacaa
     2281 acgtgaagaa gcttacgcaa gcaaacttaa aacgtcagat gtgggcggtg aaatcgattt
     2341 gttttaactt atttcaagca tattcaaacg catacggcgt tgtcgggttt tgccctctag
     2401 aggctacaac cgtctgccta gtctttatta gaatgcgcat gaaatttaga aattggtgat
     2461 taaaaaatga aaataaaagt cctagtaatt gatgactcag cgctcattcg tagtttgctg
     2521 actgaaatca tcaacagtca aaaagacatg gaagtggtag gtgccgcgcc agatccactc
     2581 attgcgcgcg atatgattaa gcaactcaac cctgatgtac tcacactgga tgttgaaatg
     2641 cccaagatgg atggcttgga ttttcttgag cgtttaatgc gcttgcggcc aatgccagtg
     2701 gtgatggtct caacgctgac tgaacgcggc tcggaaatca ccatgcgtgc gcttgagtta
     2761 ggcgcaacag attttgtgac caaacctaaa atgtcaatta ccgaaggcat gcgtgaatac
     2821 acggaagaaa ttaccgataa aattcgtgtc gcatcaaaag ctagagtgca aaccttatca
     2881 cgtcagccag tagcccaaaa caacacacca gcgccgcttc gtaacccact gattagtagt
     2941 gaaaagttat tgattattgg tgcatctact ggcggcactg aagccattaa agcattttta
     3001 ttgcaaatgc cgtcagattg cccgggcatt gtcatcacgc agcatatgcc ggcgggtttt
     3061 acaaagtcgt ttgcaaatcg gctagattcc ctttgcaaaa tttctgtcaa agaagcgcag
     3121 ggtggtgagc gtattttgcc aggccatgct tatattgccc ctggtgacaa acatttattg
     3181 cttgccagaa gtggcgcaaa ttacgtgaca caactcagcg atgagccgcc agtgaatcgc
     3241 cataaaccat cagtagatgt actgtttgat tcagcggcgg cgctggcagg taaaaatgcg
     3301 gtaggcgtaa ttttaacggg catgggcaaa gacggtgccg caggtatggc caaaatgaaa
     3361 caagcaggcg cctataactt tgcccaaaat gaagaaagct gtgtggtgta tggtatgccg
     3421 cgcgaagccg tcgctcatgg cggcgtgcat gaagttgcac atctgcagga gttacctaaa
     3481 ttggtactga attatttaac tgccaacagt gcacgcgcct tacgtgttta acgagaaaag
     3541 aaggcaattt atcacgctta tttcggctaa agtttagttg aaatatgccg ataatgaatg
     3601 aacacgataa atatatattg aaagtttagg gctttaaatt ttcaattttg caaatgacta
     3661 acggagtaga aatggccaac ccaaatacta aatttcttgt tgtagatgac ttttcaacca
     3721 tgcgcagaat tgtgcgcaat ctacttaaag aactcggcta taacaatgtg gatgaggccg
     3781 aagatggtgt ggtagcgctt agtaagctta aaggtggtgg ctttgatttt gtggtgtcag
     3841 attggaacat gccaaatatg gatggcttaa ccttgctgca acatttgcgt gcggacccag
     3901 agctaaaaca tttaccagta ttgatggtca cggctgaagc caaaaaagaa aatattattg
     3961 ccgcagcgca agcaggtgcc agcggttacg tggtgaagcc ttttacggcc gctaccttag
     4021 atgaaaagct atcaaaaata tttgagaaac tcgagaaagc agctgcctaa tgatcactag
     4081 cgaaactttg caagagccag ccacactcgt gagccacgca caagcattga ctccgcaaga
     4141 tgagatgcta agccgtattg gccatgtgac cagaactctg cacgatagtt taagaggttt
     4201 aggtctagat aaaattatgg agcaagttgc gcaggagatt cctgatgcac gtgatcgcct
     4261 gaaatatgtt gccaaaatga ccgagcaagc cgcagaacgt gtgctcaatg ccaccgatgc
     4321 cgccatgcca atgcaagatg aaatattatc gcaaggcaat gccttagaaa cgcgctggaa
     4381 agctattctt gccaacgcgt cacttaaaag tgaatacaac gaaattgcca atgaaacgct
     4441 gacttattta gcggctacag gtaaaaactc aacagaaacc aaagcgctac tcatggacat
     4501 tatgatggcg caagattttc aggatttgac gggacaagtg attaagaaaa tcactgtact
     4561 cgctcaagag ttagaacaac aacttgttca agtgctggtg gatttttcac cgggtatggt
     4621 aaaaaaagag actgaaaatc aactgatgaa cggcccgcaa attgacccag aaaatactgc
     4681 agatgtggtg gctagccaag agcaggtaga cgatttactc gatagtttgg gcttttaaaa
     4741 aaacctgagt ttatttaaaa agtttaacaa tctccaaggc ctgccctgtg agccttataa
     4801 ataaaggctt gcagggcagt atttttttct cgagcgaatt taagcagctt ttcccattaa
     4861 ataattagca ttttatgtgt tggctaactg gcttttccaa gttagataca cccggcaatc
     4921 aaactcaaac tgatggtaat caggctccat atattggcaa agttggtaaa aacttttgtc
     4981 gtgattgcgc tcttttaaat gtgctaactc atgcaccaca atcatgcgta aaaattccgt
     5041 tggtgctgcc ttaaaaaacg cagaaatacg aatctctttt ttagctttta atttgccacc
     5101 ctgcacgcgt gaaatagctg tatttagccc tagcgcatga tgctcaatgc tgagtttgct
     5161 gtcgtaatgc actttgttga gcggcggagc cgtgcgcaaa taggcctgtt taatctcatg
     5221 acaatattca tacaaggctt tatccgtttg aattgcatgt gcatttggat aacgcttggc
     5281 tatgtaatcg cccaatttat tttcagcctg caactgtaaa attttctttt gcaggtgagg
     5341 cgggtagtgt tgtagatatt tgagggtggt cattggcgta agtcaataaa tttagtcgtg
     5401 taac
//
LOCUS       DAOQSI010000138         5384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_15685_length_5384_cov_4.763558, whole genome shotgun sequence.
ACCESSION   DAOQSI010000138 DAOQSI010000000
VERSION     DAOQSI010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 5384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5384
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15685_length_5384_cov_4.763558"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PKL42_08405"
     CDS             complement(<1..564)
                     /locus_tag="PKL42_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67134.1"
                     /translation="MANEPESQVKSSVQGTESSTVARALTVTDVVRADITQDGTIVVK
                     ESLKTLTQVDVADVDLLLTFSNGDHVVIVNGALDALAPNPPSAVFSDQKILLSELFKL
                     VGVANPAKAGSLRVVTDNVEAQELACEATAVQDNPLPESAPPAPMMKVGTGTSSASKP
                     NGNGVGAGGGEGEVPATVVPLVTSQPAT"
     gene            complement(776..1996)
                     /locus_tag="PKL42_08410"
     CDS             complement(776..1996)
                     /locus_tag="PKL42_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67135.1"
                     /translation="MKTISSNPHPQINYLIFIITLVGGVLILMSISKLVHEGKQLSDF
                     SEAVRSGADPATLQTKALNYDADIAYVLRMIAASSGRAITHQEQIALESEIEKASKRL
                     SSRNALPSFLSGLLVGLGLLGTFIGLLATLNDIATLISSFGSLDMKTADPIEVFGQMV
                     KRMEAPMHSMGIAFSASMYGLLGSIILGFMMVSVRRCMGEILSILGSEVAQHIEFALS
                     RDGFAYSKTGIDKLGAGSAEQLTMRKVVSGQKTIANIGETVVGEGQVAQGIVRKEAAD
                     KKEAIRNDTSALLESIEEGDDEEFVRVLRRIEVRLAESSRLQERSLSAEVDDFQKQRA
                     DMLRTLAEQAEAMNNFRMELQRVGRQLGSVLGLMEKSNDEVVNLMREMIVRMADDTSE
                     THRLLRHQQEDRQR"
     gene            complement(2146..3393)
                     /locus_tag="PKL42_08415"
     CDS             complement(2146..3393)
                     /locus_tag="PKL42_08415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HNU67136.1"
                     /translation="MEFFNPRRSFVILVLFSLLAFVTWSSIASIDTIVRANGRIVPAG
                     KSQIVQHLEGGIVKKILVQEGEVVKAGQSLMELADVQARSSLGQEKTNLSSLRGREAR
                     LLAEATGQGKINFPKDLVDEEVKAAEIAAFNARRARLSEEARVLRDQSAQKRGDISEA
                     ESRRRNLLSELDVAKQQYRVIEGLNRDNAASHLELLDSQSRVQKLSSQIAEAESMIPR
                     LRAAVSETESRVGELWARYRSEASSELTQVRADIEKSSMVIDTNTDRLDRNIVRAPVA
                     GFINRLVVATVGGVVRPGEVLMEITPEDKNVLIEARARPNDRANLRSGLPTRVRIGAY
                     DYATYGAMLGKVTEVSADTLVDEHEGRYYRVMITVSADQKDLVVVPGMTAVADIVVGK
                     RTILSYILSPLMRFRDVAFRDPI"
     gene            complement(3431..4129)
                     /locus_tag="PKL42_08420"
     CDS             complement(3431..4129)
                     /locus_tag="PKL42_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67137.1"
                     /translation="MSIETSQEDGADIFWPGYVDAIANLVLNLLFMLTIMIVAVFMFA
                     LELSRHKENSAAVTAAESSLPTPVAALETENRQTQVNEIVQQKDAQIQQLQQQLQAAQ
                     QQALVKNTISGKQKVVIAKTPLPQPDKELERTADSGSGVVVSFENDAVTLSSSEAETL
                     RNTLAAIAANRSAKISVTVPKGFSEAKRLGFYRAMAVRNQLIEMKVPADKIDVVITEG
                     PASANNARVLVTSR"
     gene            complement(4155..>5384)
                     /locus_tag="PKL42_08425"
     CDS             complement(4155..>5384)
                     /locus_tag="PKL42_08425"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNU67138.1"
                     /translation="VLVASARVGQAKAQTGQAKAFLLPSLLLQAKGGREISSPGVQVD
                     GVTGKPESRNSHGRSDTSLTLKQPLLDLPGYYDYKRRQVVEQSRQEGQRSSEGETYLS
                     TINAYLALASSRISADMTADYEAQIQELFRYVEKRAGAGAASNSDKERVRARSLSAHS
                     SRIEQEAAHAAAGVDFVRQVNLAPSNIRLPDLEEVGLSIVPNSLDKAMELALEANPEI
                     AQLKAEMLAAELDKTAAKSRYLPRVDLELSDTNSVHAGGSNDSQRDQRLMVVMNWSLF
                     NGGGDLKYNEEKAARYEELKYRVDDQRRRVLQSLSAQYATLEATRLRIDEGYRELASI
                     TSAAQAMSKRMLSGNQSLLDMLDVYDRFYQARLRLVNLHTQEIAAVAQIARLLQGPPK
                     TSDAAIGAASPAELGVN"
BASE COUNT         1435 a         1360 c         1048 g         1541 t
ORIGIN      
        1 ggtagctggt tgcgaagtga ctaatggtac tacggttgca ggcacttcgc cttccccacc
       61 acctgcacca acgccgttac cattcggttt actagcactg gaggtgccag tacccacctt
      121 catcatcggt gctggcggcg cactttcagg taatgggtta tcttgaacag ccgttgcttc
      181 gcaggcaagt tcctgggctt caacattatc cgtgactact cttaggctgc ctgcctttgc
      241 cgggttagcc acaccaacca gcttaaataa ttctgagaga agaatttttt gatcagaaaa
      301 aaccgcacta ggtggattgg gtgccaatgc gtcgagcgcg ccgttgacaa tcacgacgtg
      361 atcgccattg ctaaaggtca gcagcaaatc aacatccgca acatcaacct gtgtcagcgt
      421 tttgagtgat tctttgacaa caatcgtacc atcttgcgta atgtctgcac gtactacatc
      481 tgtcaccgtt aacgctctag ctaccgtaga ggattcggta ccctgtacag aactttttac
      541 ttggctttct ggttcgtttg ccatttcagt atctcacttt tcaatttcgt gtagaaatat
      601 aaatttcaat ctaaatttat tgctaatcag cttaatttga gcctgttgat gactttacct
      661 taactctatt cgctaacgta atgaaaatat agcaataatc atgccttata ttaaactaaa
      721 ttgtcttaat tacaatattt ttactaaaaa ttggcagtat aaacatcaga ttattttatc
      781 tttgcctatc ttcttgctga tgcctcaaca ggcgatgcgt ttcagacgta tcatcggcca
      841 tacgcacaat catttcgcgc attaaattaa caacttcgtc gttacttttc tccatgagac
      901 ctaatacgct gcctaactgc ctaccgacac gttgcaactc cattctaaag ttattcatcg
      961 cctcagcttg ctcagccaat gttcgcaaca tatccgctcg ttgtttttgg aaatcatcta
     1021 cttcagcact gagtgagcgc tcttgcaggc gtgaagattc agctaatcgc acttcgattc
     1081 ttctcaatac tcttacaaac tcttcgtcat cgccctcttc aatagactct aagagcgcac
     1141 ttgtatcatt tcgaattgct tcttttttat cagcagcttc tttgcgaaca atgccctgag
     1201 cgacttggcc ttcaccaact acagtctcac caatatttgc aattgttttc tgaccagaaa
     1261 caacttttct cattgtcagt tgttcggcag accctgcacc taacttatca attccagttt
     1321 tactataggc aaaaccatct ctagataagg caaactcaat atgttgcgca acctctgagc
     1381 ccaatatgga caaaatctca cccatacaac ggcgcacaga caccatcata aacccgagta
     1441 tgattgagcc aagcaaacca tacatcgatg ccgagaacgc aatgcccatg gagtgcatcg
     1501 gcgcttccat acgtttcacc atctgcccaa atacttcgat agggtcagcg gttttcatgt
     1561 caagcgaacc aaaacttgaa attaacgtgg caatatcatt taaggtcgcc aatagaccaa
     1621 taaaagtacc aagcaaaccc agacccacta acaggccact caaaaatgat ggtagcgcgt
     1681 ttcggctgct taagcgtttt gaagcttttt caatttcact ttcaagtgca atttgctctt
     1741 ggtgcgtaat cgctcggcca gatgaggctg caatcatacg gagtacataa gcaatatcgg
     1801 catcgtaatt taaagcttta gtttgtaagg tagcagggtc cgcacctgat cgcaccgctt
     1861 ccgaaaaatc tgatagctgc ttaccttcat gcactaactt actgatgctc attagaatta
     1921 atacaccgcc aaccagcgtg ataataaaaa tcaaatagtt aatttgagga tgtggattgc
     1981 tagatatcgt tttcatgatg gctgaacgat aaataaatgc ggctatcaac aagaacgcga
     2041 atggcagcac cgtataaaga taataaattc ttaaacgctt atacataatc tattccacta
     2101 aaaatggttt tataaaaagt ctattcaatc aaacaaaaaa acaacctaaa tcgggtctct
     2161 aaaagccaca tctctaaacc gcatgagcgg cgataagata tatgaaagta tggtccgttt
     2221 tcctaccaca atgtcggcaa ctgctgtcat accgggaacg acaaccaagt ctttttggtc
     2281 cgcactcacg gttatcatca ctctgtaata tcgtccttca tgctcgtcca ctaaggtatc
     2341 cgcactcact tcagttactt tacctagcat tgccccgtaa gttgcataat cgtatgcgcc
     2401 tatccgcaca cgcgtaggca aaccactacg caaattggcg cgatcattag gccttgcgcg
     2461 cgcttcaatt aatacatttt tatcttcagg tgtaatttcc atcaacactt cacctggacg
     2521 caccacaccg cccaccgtcg caactacaag ccgattgata aaacctgcaa ctggtgcacg
     2581 tacaatatta cggtctagcc tatcggtatt ggtatcaatg accatagaag acttttcaat
     2641 atctgctctc acttgcgtga gctcagaaga tgcttcagaa cgataccttg cccataactc
     2701 gcctacacga gactcggttt cagacactgc tgcccgtagc cttggaatca ttgattccgc
     2761 ctctgcaatt tgacttgaaa gcttctgcac ccgtgattga ctatcaagca actctaaatg
     2821 agaagccgca ttatcacgat taaggccttc aataacacgg tactgctgtt ttgcaacgtc
     2881 taattcagaa agaagattcc gccgcctgct ttctgcttca gaaatatcac cacgtttttg
     2941 tgcactttgg tcacgtaaca ctcttgcttc ttcagaaagt cttgcacgcc ttgcattaaa
     3001 tgcagcgatt tcagccgctt taacttcttc atccaccaaa tccttaggga agttaatttt
     3061 tccttgccct gtcgcctcag ccagtaatct cgcttccctg ccacgaaggg atgataaatt
     3121 ggttttttct tgccctaggc ttgaccttgc ctgtacatcg gctaattcca tgagtgattg
     3181 ccccgcctta acgacctcac cctcttgcac cagaattttt ttaacaattc cgccttctaa
     3241 atgctgaaca atttgcgact ttccagcagg aacaatacgc ccatttgcgc gcacgattgt
     3301 gtcaatgctt gcaatgctac tccatgtaac aaaagccaag aggctgaaca aaacaaggat
     3361 cacaaaactt cgacgcggat taaaaaattc catttttttc taattccaca tcgttattcg
     3421 aaccgcacag ctatcgtgaa gtcaccaaca cacgtgcatt attggcactt gctgggcctt
     3481 cagtaataac cacatcaatt ttgtctgctg gcaccttcat ctctatgagc tgatttcgta
     3541 cagccatcgc gcgataaaag cctagacgtt tagcctcaga aaaacctttt ggcacagtga
     3601 cggaaatttt tgccgagcga ttggcagcaa ttgcagctaa ggtatttctt agtgtttcgg
     3661 cttctgaact gcttaaagtg accgcatcgt tttcaaagct caccaccaca ccagagcctg
     3721 aatcggcagt acgttcaagt tctttatccg gttgaggaag cggtgttttt gcaatcacca
     3781 ctttttgctt acccgaaata gtatttttga ctagagcttg ctgctgcgcc gcctgtaatt
     3841 gttgttgcaa ttgctgaatt tgtgcgtctt tctgctgcac aatttcattg acttgggttt
     3901 gtctattttc agtttccagc gcagcaacgg gcgtgggtaa gctagattct gcagcggtaa
     3961 cagcagctga attttcttta tggcggctta attcaagggc aaacatgaaa accgccacaa
     4021 tcatgatggt taacataaaa agcagattta gtactaagtt ggctatcgca tcaacatagc
     4081 cgggccaaaa aatatcagcc ccatcttctt ggctagtttc aatagacatg cattaaccct
     4141 caatatttct catattagtt cacacccagt tctgcgggcg atgcagcacc aatagctgca
     4201 tccgacgttt ttggcggccc ttgtaagagc cttgcaattt gtgctacagc cgcaatttct
     4261 tgtgtgtgta aattcaccag acgtaaacgt gcttggtaaa accgatcata tacatcgagc
     4321 atatctagca gcgattgatt acctgaaagc atacgttttg acattgcctg tgcggcagag
     4381 gtgatggaag ctaactcacg atagccttca tcaatgcgca agcgtgtcgc ttccagggtt
     4441 gcgtattgcg ctgaaaggct ttgtaacacg cgacggcgct gatcatctac gcgatatttt
     4501 aactcttcat aacgggcggc tttttcttcg ttatatttaa ggtcaccgcc accattaaat
     4561 aaagaccaat tcattaccac cattaagcgc tgatcacgtt gactgtcatt tgaaccgcca
     4621 gcatgcacgc tattggtatc tgaaagctct aaatctaccc ttggtaagta acggcttttg
     4681 gcagcggttt tatcaagttc agccgcgagc atttctgctt taagctgggc aatttcagga
     4741 ttggcctcaa gcgctaactc catcgcttta tccagcgagt ttggcacaat tgacagcccc
     4801 acttcttcca aatcaggtag ccgaatattg ctaggtgcta aattcacctg ccgcacaaag
     4861 tcaactccag cggcggcatg tgctgcctct tgctcaatac gtgaagaatg ggcgcttaaa
     4921 ctacgtgcac gcacgcgttc tttgtcagag ttgctggccg cccctgcccc agcgcgtttt
     4981 tcaacatagc gaaatagttc ttgaatttgc gcttcgtaat cggctgtcat atcggcagaa
     5041 atgcggctag aagccagtgc tagataggca ttgatggttg aaagataagt ctcgccctca
     5101 cttgaacgct gaccttcttg acgagattgc tctaccacct gacggcgttt gtagtcatag
     5161 taaccaggta aatccagcaa aggctgtttt agcgttaagc tggtatcact ccgtccatga
     5221 ctatttctag attcaggttt tcccgtgacg ccatcaactt gaacaccagg ggatgaaatt
     5281 tctctaccgc ctttggcttg caataacaat gatggcaata aaaaggcctt tgcttgcccg
     5341 gtttgcgctt tggcttgacc tacgcgggca gaggctacta aaac
//
LOCUS       DAOQSI010000139         5352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_15836_length_5352_cov_5.031338, whole genome shotgun sequence.
ACCESSION   DAOQSI010000139 DAOQSI010000000
VERSION     DAOQSI010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15836_length_5352_cov_5.031338"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1349
                     /locus_tag="PKL42_08430"
     CDS             <1..1349
                     /locus_tag="PKL42_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HNU67139.1"
                     /translation="WLEREGPRKAGLVSMLCWCGGLLISAYGVYAHQLWMMWLGSGVI
                     GGIGLGLGYISPVSTLIKWFPDRRGMATGMAIMGFGGGAMIGSPIAAKMMSYFSTPTS
                     VGVWQTFVALAAIYFVFMLWGALSYRVPATGWKPANWTPPATQNNAMISSKHVHLKNA
                     HKTPQFWLLWLVLCMNVSAGIGIIGAAAPMLQETFGGALIGQPEVGFAEVKKDETMTA
                     AAAAIGAGFVGLISLFNIFGRFAWATSSDKLGRKNTYYIFFVLGAIMYCAATYVAGFK
                     SLALFVAAFCVIASMYGGGFATIPAYLADMFGTQFVGAIHGRLLTAWSTAGILGPVVV
                     NYLHDTRLEANVPFDKIYAPIFLVLAAMLVIGFIANWLVKPVADKYYMTDAELDEVRK
                     AAHEKSATANAGNASANGQVQVASHPILVVIAWALVLIPISYGVWSTLQKAWGLFQ"
     gene            1430..1879
                     /gene="ribH"
                     /locus_tag="PKL42_08435"
     CDS             1430..1879
                     /gene="ribH"
                     /locus_tag="PKL42_08435"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNU67140.1"
                     /translation="MRELEKNLDGTNLRVGVVLSRFNSDIGDGLLSACTAELLKLGVA
                     SEAITIATVPGALETPLILQHMAMSDQYDALIALGAIIRGETYHFEVVANESARGISE
                     VQFNAGIPVANAVLTTEDDDQAIARMHVKGAEAAQVAVEMANLIRAL"
     gene            1879..2403
                     /gene="nusB"
                     /locus_tag="PKL42_08440"
     CDS             1879..2403
                     /gene="nusB"
                     /locus_tag="PKL42_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNU67141.1"
                     /translation="MSNQESGTTQKAVKSLVIENIPTKKPHAKKNAGKNRRKSRELVM
                     KAVYRGMLNQSDINAVLRDMTEDPDYNKADEAYFKHLLSEVNLHMAALDEKMAQFIDR
                     KVTELSPVEHAILRISSCELMFDLSIPYRVVINEGVELAKLYGGLDGHKYINGVLDKL
                     AAEVRAAEVKSVRA"
     gene            2477..5005
                     /locus_tag="PKL42_08445"
     CDS             2477..5005
                     /locus_tag="PKL42_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNU67142.1"
                     /translation="MKKNFWKSDWFAVLIVSMAFLLATNSDILQSLERKAYDFGVQAS
                     ARDAGNHIAIIAIDDQSIANIGRWPWSREVHATMLSKLALASPKVIGSTVYFLEPQID
                     PGLSYINQISDFVTSSSILNATPEASQLDALLKEAKASLNADAKLVASIQQANNVVLA
                     MPFTLGEPRGNLDKPLPEYVRKNTLVNIVDKIEAEQQGQLPIPAMAAIAPFAEVGKVA
                     VAIGHLNANPDVDGAIRSEPLVLSFYNQYYPSLSLQIAAQYLNLKPQDIKVRLGEGLV
                     LGKLNISTDSALQMNTFFYSDKDGKAPFVADSFYDVLSGKISTEKYKDKIVLIGMAAT
                     GDGSDNQITPISSHMKPIETLAHSVASILNEDFFVMPAWAIWGQLSAWLLVAMYLSVL
                     LPRLGVGRAAMTTLSFFAVLVTLHLWLMTQKGLWLPLTMPAALLMFGHAVLTTKRFFL
                     TEQGKAKSDADSAESNRNLALMFQSQGQLDMAFDRFRKCPMDESVMEGMYTLGLDFER
                     KRQFNKAEAVFAYLAKHDADYKDLALRVAHARAMAETIIVGSGGTRTNASTLILNQEG
                     IEKPMLGRYQIEKELGKGAMGVVYLGRDPKIGRVVAIKTMDLSQAFEEDAIDEAKRRF
                     FREAETAGKLNHPNIVTIYDAGEEHDLAYIAMEFLKGNDLTAYIKPDNLLPLNTVTEI
                     ICKLADALDYAHTQHVVHRDVKPANVMYAPETGLVKMTDFGIARITDASKTRTGLILG
                     TPSYMSPEQLSGKKIDGRSDLFSLGVMFYLLATGQLPFEGESMAALMFKITNEPQVSA
                     HLLNAAVTPCLSAVIDRMLEKDMDKRYATGQEIVRDIKRCLQTG"
     gene            5024..>5352
                     /locus_tag="PKL42_08450"
     CDS             5024..>5352
                     /locus_tag="PKL42_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67143.1"
                     /translation="MSLANVLESDVLEIVRLTDVGLLREHNEDTIASDSTIGYVVLAD
                     GMGGYKAGEVASEIAVLSIAAELTEVMLNQPPLESDQYLQYQDHALNAMQPEAQLLVD
                     AVKNANDA"
BASE COUNT         1422 a         1095 c         1383 g         1452 t
ORIGIN      
        1 gctggttaga gcgcgaaggt ccgcgtaaag cgggtctggt ttcaatgctg tgctggtgcg
       61 gcgggttgtt aatttcagcc tatggcgttt atgcgcacca gttatggatg atgtggctcg
      121 gaagcggcgt cattggtggc attgggctag gtctgggcta catatcccct gtttcaacgc
      181 tgattaaatg gtttccagac cgtcgtggca tggccaccgg tatggcgatt atgggttttg
      241 gtggtggcgc catgattggt tcgcctatcg ctgctaaaat gatgagctat ttttccacgc
      301 ccaccagtgt tggcgtttgg caaacgtttg tggcactcgc tgccatttac tttgtgttta
      361 tgctctgggg cgcactgagt taccgcgtac cggcaaccgg ctggaaaccc gctaactgga
      421 cgccgcccgc gacacaaaat aacgccatga tttcaagcaa gcatgtgcac cttaaaaatg
      481 cccataaaac cccacagttt tggttgctat ggctggtgct gtgcatgaac gtgtcggcgg
      541 gtattggcat tattggtgcg gccgcgccaa tgttacaaga aacttttggt ggcgccttga
      601 ttgggcagcc agaagttggt tttgctgagg ttaagaaaga cgaaactatg acggcagcgg
      661 ctgctgcgat tggtgctggg ttcgtaggtt tgatttcatt atttaacatt ttcggccgct
      721 ttgcctgggc aacatcgtca gacaaattgg gccgtaaaaa cacctactat attttctttg
      781 tactcggtgc cattatgtat tgtgccgcga cctatgtagc agggtttaaa tcgctggcgc
      841 tgtttgttgc ggcgttttgc gtgattgctt ccatgtatgg tggtggtttt gcaaccattc
      901 ccgcctattt agcggatatg tttggtacgc aatttgtggg cgcgattcat ggccgtcttc
      961 tgacggcatg gtctactgcc ggcattctag gcccagtggt ggtgaactat ctgcacgata
     1021 cacgccttga agccaacgtc ccgtttgata aaatctacgc ccccatcttt ttagtgctgg
     1081 ctgccatgct ggtgattggg tttattgcca attggctagt taaacccgtt gccgataaat
     1141 actacatgac cgatgcagaa ttagacgaag tgcgtaaagc tgcacatgaa aagtcggcca
     1201 ccgcgaatgc aggcaatgca tcagcaaacg gccaggtgca agtggcgagc catccaatat
     1261 tagtggtcat cgcttgggcg ttagtgctga ttccaatcag ctatggggtt tggagtacgt
     1321 tgcaaaaagc ttggggctta tttcagtaac ccactttttc tcaaacgtgc aggcaatata
     1381 cggtaaaata cgcctttatg atttgcaggc ctgaataaag gaattaagcg tgagagaatt
     1441 agaaaaaaat ttagacggca ccaatttaag agttggtgta gtgctatcaa gatttaatag
     1501 tgatattggg gacggtttat taagtgcttg cacggctgaa ttgcttaaat taggcgtggc
     1561 atctgaggca attaccatcg ccaccgtgcc tggtgcgtta gaaacaccac tcattttgca
     1621 gcatatggca atgagcgacc aatatgatgc actcattgcc ctaggagcga ttattcgcgg
     1681 cgaaacgtat cactttgaag tggttgccaa tgaatcagcg cgcggcatct ctgaagtgca
     1741 gtttaatgca ggtattccag tagccaatgc tgtgctgacc actgaagatg atgaccaggc
     1801 gattgcacgc atgcacgtta agggcgccga ggcggcacaa gtggcggttg aaatggccaa
     1861 cttgattagg gcgctgtaat gagcaatcaa gaatcaggta ctacgcaaaa agcggtgaag
     1921 agtcttgtga ttgagaatat accgacaaaa aaaccccatg caaaaaaaaa tgccggtaaa
     1981 aatcgtagaa aatcacgtga gttagtcatg aaggccgtgt accgcggcat gctgaatcaa
     2041 tcagatatca atgcggtttt gcgtgacatg accgaagacc ctgattacaa caaagcagac
     2101 gaagcctact tcaagcattt gctttcagaa gtgaatcttc acatggcggc gttggacgaa
     2161 aaaatggcac aatttatcga tcgtaaagtc actgaactta gcccagtgga gcatgccatt
     2221 ttgcgcattt ctagctgtga attgatgttt gatttaagca ttccttaccg cgttgtgata
     2281 aatgaaggcg ttgaactggc aaagctgtat ggcggccttg atgggcataa atatatcaat
     2341 ggggtgctag ataagctggc agcagaggtc agggcggcag aagttaaaag tgttagagcc
     2401 taaggcgcac tgaaattatg cgcactgagc gattgatcat tgacgactga aaaggttgat
     2461 ggcttaaggc ttgcgaatga aaaaaaactt ttggaaatct gattggtttg cagtactcat
     2521 cgtttcaatg gcttttttac tcgcgaccaa tagcgatatt ctacaaagcc tagagcgaaa
     2581 agcttatgat tttggcgtac aagcaagtgc gcgagacgct ggcaatcata ttgccatcat
     2641 tgccattgat gatcaaagta tcgccaatat tggccgctgg ccgtggtcgc gtgaagtgca
     2701 tgcaacgatg ttgtcaaagc tcgctttggc ttcccctaag gtcattggaa gtaccgtcta
     2761 ttttcttgag ccgcaaattg atcctggctt aagttatatc aatcaaattt ctgattttgt
     2821 tacttcgtcc agcatcctga atgcaacgcc agaggcatcg cagttggatg ctttattaaa
     2881 agaggccaag gcatctttaa atgcagatgc aaaacttgtt gccagtattc agcaagccaa
     2941 taatgttgtt ttagccatgc cctttacgtt gggcgagccg cgaggcaatc tcgacaagcc
     3001 gctaccggaa tatgtacgca aaaataccct tgttaatatt gtagataaaa ttgaggctga
     3061 acaacaggga cagctaccca tacccgcgat ggcggccatc gccccttttg cagaagtggg
     3121 taaggtggca gttgcgattg gccacctgaa tgcgaaccca gatgtcgatg gcgcaattcg
     3181 ttctgaaccg ttggtgttat cgttttacaa tcaatattac ccttcgctct cgctgcaaat
     3241 cgctgcgcag tatttaaacc tcaaaccgca agatataaaa gtacgtttag gtgagggcct
     3301 tgtgctgggt aaactcaata tctccaccga tagcgcatta caaatgaaca cgttttttta
     3361 ttcagataaa gacggtaaag caccgtttgt agcagattct ttttatgatg tgttgagcgg
     3421 caaaatatca accgaaaaat acaaagataa aattgtgtta attgggatgg cagcaactgg
     3481 cgatggcagt gataatcaaa tcacgccaat atcatctcac atgaaaccca ttgagacact
     3541 cgcacattct gtggcgagta ttttaaatga agattttttt gtaatgcctg catgggccat
     3601 ttggggacaa ctaagtgctt ggctgttagt cgccatgtat ttgagtgttt tgttgcctcg
     3661 actaggggta gggcgcgcgg caatgacaac gttaagtttt tttgcagtgt tggttacatt
     3721 gcatctttgg ctgatgacgc aaaaggggtt gtggttacca ttgacgatgc ctgcggccct
     3781 gttgatgttt gggcatgccg ttttaacgac gaaacggttc tttttaacgg agcaaggtaa
     3841 ggctaaatct gatgctgact cagccgaaag taaccgaaac ttagccttga tgtttcaaag
     3901 tcagggtcag ttggatatgg cttttgaccg ttttcgtaaa tgcccgatgg atgagagtgt
     3961 gatggaaggt atgtatacgt taggcctaga ttttgagcgc aaacgccaat tcaacaaggc
     4021 tgaggccgtg tttgcctact tggccaagca tgatgcagat tacaaggatt tggcgctccg
     4081 agttgcacat gctagagcga tggctgaaac gattattgtc gggagtggtg gaactcgaac
     4141 aaacgcaagt acgctgatat taaatcaaga gggcattgaa aaaccaatgt tggggcgtta
     4201 tcaaattgaa aaagaactcg gtaaaggtgc aatgggcgtg gtttatttag gtcgcgaccc
     4261 caaaattggc cgagtggtgg cgattaaaac catggatttg tctcaagcat ttgaagaaga
     4321 tgcaattgat gaagcaaaac ggcgtttttt tcgagaggca gaaacggctg gcaaactcaa
     4381 tcatccaaat attgtgacca tttacgatgc aggtgaagag catgacctag cctatattgc
     4441 aatggagttt ttaaaaggta atgatctaac tgcatatatt aagccggata atttactgcc
     4501 tttaaatacg gtcactgaaa ttatttgcaa gttggcggat gccttggact atgcgcatac
     4561 acaacatgtg gtgcatcgtg atgttaagcc cgcaaatgtg atgtatgcgc ctgaaacggg
     4621 tttggttaaa atgacagact ttggcattgc ccgtattaca gatgcctcta aaaccagaac
     4681 aggcctcatt cttggcaccc ctagctatat gtcgccagag cagttgtccg gcaaaaaaat
     4741 tgacgggcgt tctgatttat tttcattagg ggtgatgttt tacctgttag ccacggggca
     4801 attgcctttt gagggtgaat cgatggctgc attaatgttt aaaatcacca atgagccaca
     4861 agtgagcgca catcttctga atgccgcagt gacgccatgt ttatctgcgg taattgatcg
     4921 tatgctagaa aaagacatgg ataaacgcta tgccactggc caagaaattg tgcgtgatat
     4981 taaacgttgc ctgcaaacag gttaatatag caaactgatg aatatgagct tagcaaacgt
     5041 gcttgaaagt gatgtattag agatagtccg gcttactgac gttggtttgt tgcgtgagca
     5101 taatgaagac accatcgcca gtgatagtac tattggctat gtggtgctgg ctgatggtat
     5161 gggcgggtat aaagcaggcg aggtcgctag tgaaattgcc gtgctcagca ttgccgctga
     5221 actcacagaa gtgatgctca atcagccccc cttagagtca gatcaatatc tgcaatatca
     5281 agaccatgcg cttaacgcca tgcagccaga ggcgcaactg ctagtagatg cggtaaaaaa
     5341 tgccaatgat gc
//
LOCUS       DAOQSI010000140         5335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_15912_length_5335_cov_5.333712, whole genome shotgun sequence.
ACCESSION   DAOQSI010000140 DAOQSI010000000
VERSION     DAOQSI010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15912_length_5335_cov_5.333712"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..1342
                     /locus_tag="PKL42_08455"
     CDS             140..1342
                     /locus_tag="PKL42_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNU67144.1"
                     /translation="MISLAGRDIMHAWGKFVFTGVGLGLLIGVTLTMAGVYRGMVDDA
                     KALLDNSGADLWVVQKDTLGPYAESSSLYDDTYRSLLSISGVARAANITYLTMQVREG
                     GRDVRAMVVGVVSGDLGQPGQPSYLINGRQITRGHYEAVADIASGFNLGQSIQIRRNN
                     YTVVGLTRRMVSSGGDPMVFIPLKDAQEAQFLKDNDAIVQQRRRTAENPAFNRPNVPG
                     LLDQVIASQSTNAFVNAVLVQIKPGFNAEEVAEPIRHWKRLQVYTRTQMEGILVGKLI
                     ATSAKQIAMFLVILAIVSAAIVAFIIYTLTMGKIREIAVLKLIGTKNRTIAGMIMQQA
                     LGLGVIGFVVGKVAATFWAPIFPKYVLLEPGDAIRGFFVVMLICILASLLAIRAALKV
                     DPAEAIGG"
     gene            1347..2072
                     /locus_tag="PKL42_08460"
     CDS             1347..2072
                     /locus_tag="PKL42_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNU67145.1"
                     /translation="MAELKQESKGIHIEGLRKRYGEGDTAVDALKDVNMIVSPGEVVG
                     LVGPSGSGKSTLLKCLGAVIEPSAGRMTLGDQVIYDNGWQIKDLRALRRDKIGFVFQA
                     PYLIPFLDVTDNVALLPMLAGHANTDSRQRALALLEALDVAHRAKAMPSQLSGGEQQR
                     VSIARALVNRPPVILADEPTAPLDSERALAVIRILNQMALQYQTAIIVVTHDEKIIPT
                     FKRIYHIRDGKTHEEAGEGRAFE"
     gene            complement(2409..2915)
                     /locus_tag="PKL42_08465"
     CDS             complement(2409..2915)
                     /locus_tag="PKL42_08465"
                     /inference="COORDINATES: protein motif:HMM:NF018926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HNU67146.1"
                     /translation="MTTLHATPYNIDATGFYFTDAHDYEAKSAVHVDRYGNLVEEFEI
                     QFIDGDDAQLFEVCGINQANLNIWFDDIEFLNDYEKVNLYYLAGVVGYTLSQALEKLD
                     EPSITESSLRDAAEELFNECWLPSFPESIRFYIDYDKFARDCELSGDMTEFEYNNKTY
                     TCTNSSAL"
     gene            complement(3416..4159)
                     /gene="istB"
                     /locus_tag="PKL42_08470"
     CDS             complement(3416..4159)
                     /gene="istB"
                     /locus_tag="PKL42_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011544395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HNU67147.1"
                     /translation="MLSQQTINKLSEMNLKGMSKGYEEQLSSAACQGLAFEDRFGMLV
                     DQEETYRENQRLVRLLKSAKLKENACMEDIDYSHSRGLDKAQIASLNSTDWIKRGFNL
                     LITGPTGCGKTWISCALGNQACRLGLSVSFQRLPLLLEDLHLAHADGSFRKKLMQLAK
                     VDLLILDDFGIATLNAQNRGDLLEVIDSRAGVKSTAITSQIPVELWHDYLKGGNPTIA
                     DATLDRLVSGTIRIQLKGESMRKVRAGKI"
     gene            4254..4538
                     /locus_tag="PKL42_08475"
     CDS             4254..4538
                     /locus_tag="PKL42_08475"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNU67148.1"
                     /translation="MRGIRYTDEFKAEALKQITERGHSAVEVAKRLGITDKSLYRWKA
                     EAEQQLKQPDGQDVASLKLEIVKLNKALKRATEEREILKKAAVYFANHPE"
     gene            4550..5287
                     /locus_tag="PKL42_08480"
     CDS             4550..5287
                     /locus_tag="PKL42_08480"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HNU67149.1"
                     /translation="MQAHSKLFRVNTMCHVLNVNRSGYYAWSNKPASARALDNALLLD
                     EIKQFYAVSGNIYGSPRIYRDCKAAGLACSENRVAKLMHQAKLHAVRGYRRARHKAGK
                     PAIAAPNRLRQVFVATTPDEAWVTDITQIYTHEGWLYLAVVIDLYSRAVVGWSMQGHI
                     RTELVLDAMLMAKWRRQPKQPVVIHSDQGSQFGSDDFNRWCKENNFVTSMSRRGNCYD
                     NAAMESFFSSLKKEKTRGKIYNTREEC"
BASE COUNT         1484 a         1165 c         1239 g         1447 t
ORIGIN      
        1 ttgatggtaa cgtgcaggta gttgaaggcc ttaaagctgg acaaaaagta gtactttata
       61 gccaacatgc gcttaaggtc agcagtcgaa ttaaaatagt aaagcagatc cctggcgcac
      121 caacctctgg ctctaaaccg tgattagtct tgctgggcgt gacattatgc acgcttgggg
      181 taagttcgta ttcactggcg taggtctagg tttgctaata ggcgtcacgc tcacgatggc
      241 aggtgtctat cgcggcatgg tcgacgatgc gaaggcctta cttgataata gtggtgccga
      301 tttgtgggtg gtacaaaaag ataccttagg cccttatgcc gaatcatcaa gcctttatga
      361 tgatacctat cgcagtttgc ttagtatctc cggtgtggcg cgtgctgcca acattactta
      421 tctcacaatg caagttcggg aaggtgggcg tgatgtacgt gcgatggtcg ttggcgtagt
      481 ttctggcgat ctaggtcaac ctggccaacc tagttacctc atcaacggca ggcagattac
      541 tcgcggacat tatgaagcgg ttgctgatat tgcctcaggg ttcaatctag gccaaagcat
      601 acagattcga cgtaacaact ataccgttgt tggtctcacg cggcgcatgg tttcatcagg
      661 cggcgacccg atggtgttca ttccacttaa agatgcacaa gaggcgcaat ttttaaaaga
      721 caatgatgcc attgtgcaac aacgccgccg taccgctgaa aacccagctt ttaatcgccc
      781 caatgtgccg ggattacttg atcaagtgat tgcctctcaa agcaccaatg cttttgtgaa
      841 tgcagtattg gtacagatta aacctggatt taacgcagag gaagtggctg aacccatacg
      901 ccattggaag cgcctgcagg tttacacgcg tacgcaaatg gaaggcattt tggttggtaa
      961 actgattgct acatcagcca agcagattgc catgtttctc gtaattttag ccattgttag
     1021 cgccgcaatt gtcgccttca ttatctacac actcaccatg ggtaaaatac gcgagattgc
     1081 ggttctgaaa ttaattggta ctaaaaaccg cactattgcc ggcatgatta tgcagcaggc
     1141 gctgggtctt ggtgttattg gttttgtcgt aggtaaagtg gccgccacat tctgggcgcc
     1201 aatctttcct aaatacgtac tgcttgaacc tggcgatgcg atacgtggat tttttgtagt
     1261 catgcttatc tgcatattag ccagcttgtt ggctatccgt gccgcactta aagtagatcc
     1321 agcggaggct atcggtggct aatgacatgg cagaacttaa gcaagagtca aaaggcatac
     1381 acattgaagg cctacgtaag cgttacggcg aaggtgacac cgcggttgac gcgcttaaag
     1441 atgtaaacat gatcgtctcc cctggtgaag tggtcggttt ggttgggcca tcagggtctg
     1501 gtaaaagtac gctacttaaa tgccttggtg cggtgattga gcccagtgca ggacgaatga
     1561 cgctaggtga tcaagtaatc tacgataacg gttggcagat taaagaccta cgtgcattac
     1621 gtcgagataa aattggcttt gtattccaag cgccctatct tattccattt cttgacgtga
     1681 cagacaatgt ggcgttgtta cccatgcttg ctgggcatgc caatacagac tctaggcagc
     1741 gcgcacttgc cttattagaa gcgctggatg ttgcacatcg tgccaaagct atgccatcac
     1801 agttatccgg tggtgagcag caacgggttt ctattgcacg cgcattggtc aatcgacccc
     1861 cagtgatact tgccgatgag cctactgcgc cacttgatag tgagcgtgct ttagcggtaa
     1921 tccgaatctt gaatcaaatg gcattgcagt accaaaccgc cattattgtg gtaacgcacg
     1981 atgaaaaaat cattccaaca tttaagcgta tttaccatat ccgagatgga aaaacgcatg
     2041 aagaagctgg cgaggggcga gcttttgagt aatgtttagt tgttatataa ggatagagaa
     2101 tgtcaactat gggcccttag tgtcgaacat gagcggccgc tatgcagttc cacagtttat
     2161 atttttgcca gctactgttt aagaacaaat cagtttgttt aaaagtgaaa gaatggtcag
     2221 tttcaatatt aattagtttc agctggattg gatgattatt cgtatagctc aaaataaaat
     2281 gccaacagcg tcaaatattt tttgagatca taaatttaat taaaagccta agtaattggc
     2341 cgcaagccaa aaaaatatct tatttcatgc ctgctgacct aaatcagaag gcatgttgct
     2401 tttagcaatt acaaagcact agagttagta caagtgtagg ttttgttgtt gtattcaaac
     2461 tccgtcatat cgccactcag ttcgcaatct ctggcaaatt tgtcataatc gatataaaaa
     2521 cgaatactct caggaaagct aggtaaccaa cattcattaa acagctcttc ggctgcatcg
     2581 cgtaagctag actctgtgat gctaggttca tccagttttt caagcgcttg gcttaatgtg
     2641 taccccacca ccccagccag ataatataga tttacttttt catagtcatt taaaaactca
     2701 atatcatcaa accatatatt cagattagct tggttaatgc cacaaacttc aaatagctgc
     2761 gcgtcgtcac cgtcaataaa ctgtatctcg aattcttcta ctaaattgcc gtaacggtca
     2821 acgtgcacgg ctgatttggc ctcatagtcg tgtgcatcgg taaaataaaa acccgttgcg
     2881 tctatgttat agggtgttgc gtgtagtgtt gtcatagtct tttgcctcac tgtatcgttc
     2941 ttagattgaa gagccgcttt tgaattaaag acttgccccg aatttgcaga cttatcaacc
     3001 cgacctgcat cgctcttgag ttgtttcgcc tgccgataag gttcatggag attaagaaag
     3061 aaaagccagc aaccagccaa aatcgcagtc gcaaagccga tatgcgaacc gattgcaggt
     3121 ggtcacgaag tgtttatgct tgtttggatt tcgccccgtg agatggtcgg tgctttggcg
     3181 aaactaaaga gcgaatgctg ttaatagatg aatagcaaag caggggatgt ttttagtgcg
     3241 tattactgtc taaaagtgcc actcaatatc acgcaaaata gccacctata actgttcaaa
     3301 gttgccacac attactgtac aaaaaagcca cttcctaaat tactagaaag tggctttcat
     3361 ttcactacgc ataagaactc ttgggccaat caattttatg cgttgttaag acggtttata
     3421 ttttacccgc ccttacctta cgcatagatt ctccctttag ctgaatacga attgtgccgc
     3481 tcacaagtct atcaagagtg gcatcggcaa ttgtaggatt accgcctttt aaataatcgt
     3541 gccataactc cacggggatc tggcttgtaa tggcagtaga cttcacgcca gcgcgactat
     3601 caatcacctc aagaaggtca cccctgtttt gggcatttaa tgttgcaatc ccaaaatcat
     3661 ccaatatcaa caggtcaact ttggcgagtt gcataagttt tttacggaaa ctaccgtcgg
     3721 catgtgccaa atgcaagtct tccaacaaaa gtggcaaacg ttggaagcta acggacaatc
     3781 ccagtctgca agcctgatta cctaatgcgc aggaaatcca tgtcttacca cagcccgttg
     3841 gacctgtaat aaggagatta aagcctcgct taatccagtc tgtactattt aagcttgcaa
     3901 tttgcgcttt gtctaggcca cggctatgac tatagtcgat gtcctccata caggcatttt
     3961 cctttaattt ggccgattta agtaacctga ccagccgttg gttctcacgg taggtctcct
     4021 cttgatcgac cagcatgcca aaacgatctt cgaatgccag tccttggcaa gcagcactgg
     4081 aaagctgctc ctcatagcct ttgctcatgc ctttaagatt catttcggat agcttattga
     4141 tggtttgttg cgataacatt gtgtaatcct ttttatatta aattagtggt agttggactt
     4201 cccccgattt agtagacagt tgataatgtc tgaaatcact agaaggaaga taaatgagag
     4261 gcatcagata taccgacgag tttaaggcag aagcgcttaa gcaaattaca gaacgaggtc
     4321 acagcgctgt tgaagttgct aagcggctag gcatcactga caagagctta tatcgctgga
     4381 aggctgaagc agaacagcaa ttaaagcagc ctgacggcca agatgttgcc agtctaaagc
     4441 ttgagatagt caaactcaac aaagccttaa aacgggccac agaagagcga gaaatcctaa
     4501 aaaaggccgc ggtgtacttt gcaaatcacc ccgagtaaag tacaaattca tccaagcaca
     4561 cagcaagctg tttagggtta atacgatgtg tcatgtgctg aatgtgaatc gtagcggtta
     4621 ttacgcatgg agcaacaaac cagcatctgc acgtgcatta gataacgcgc tgttgttaga
     4681 tgaaatcaaa caattctatg cggtcagtgg caacatctac ggcagcccaa ggatctaccg
     4741 tgattgcaaa gccgcaggct tagcctgtag cgagaatcgt gtcgctaagc tgatgcatca
     4801 agctaaactt catgcagtgc gtggctatcg ccgtgctcgc cacaaagcag gcaagcctgc
     4861 gattgccgca ccaaaccggt tgcgccaagt atttgtggca acaacacctg atgaagcttg
     4921 ggtcaccgac attacccaaa tatacaccca tgagggttgg ctatatttgg ctgtggtgat
     4981 tgatttgtac tcacgtgcag tggtgggctg gagcatgcag gggcacattc gtacagaatt
     5041 agtgttagat gcgatgctga tggctaaatg gcgcaggcag cctaaacaac ccgtggtgat
     5101 ccactcggat caaggtagcc agtttggcag tgatgatttt aatcgctggt gcaaggaaaa
     5161 caactttgtg accagcatga gtcgtcgtgg caattgctat gataatgcag caatggagtc
     5221 gttcttcagt agcctaaaga aagagaaaac caggggtaaa atttacaaca caagagagga
     5281 gtgttaaatt gcatcgaata ttgacccatc tagcatagaa atatgcatct aaatt
//
LOCUS       DAOQSI010000141         5323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_15966_length_5323_cov_4.857631, whole genome shotgun sequence.
ACCESSION   DAOQSI010000141 DAOQSI010000000
VERSION     DAOQSI010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 5323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5323
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15966_length_5323_cov_4.857631"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1841
                     /locus_tag="PKL42_08485"
     CDS             <1..1841
                     /locus_tag="PKL42_08485"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNU67150.1"
                     /translation="EDPVVAELLNAHFINIKVDREERPDIDQIYQTAHSMLSQKSGGW
                     PLTVFLTPNQQPYFTGTYFPKAARYQLPGFADLIPRVAAYYHEHRADINIQTQQLASV
                     LARTIPMANTTVTADEHTIELAFEQLRESFDFEHGGFGSSPKFPNPADVTLLLHHAKS
                     GNSQAEAMALQMLTAMASGGIYDHIGGGFCRYSVDERWNIPHFEKMLYDNGQLLCLYT
                     DGWQISQNPRYEVVVEETVAWLICEMRHASGAFYSSQDADSLDAHGHAEEGAFYVWQR
                     EEVKALLTPEEFIAASRCFGFDRAANFENPHSGVRAWHPYLTMAPEDDEYVALNNART
                     KLFEAREKRSRPASDDKILTSWNALAIKGLARAGRVFNRTDWIAFAQNAVDFVRENMW
                     VNAQLLATSKDGKAHLNAYLDDYAFLLDALLELLQADYRSVDMQFAEDIADVLIENFE
                     AEDGGFYFTAHHHEALIHRPKQAYDNATPNGNGIAAGALQRLGHLLGEPRYLQKAERT
                     LQAFDSVVKRNPAACANLSEALREYLTPPTIVILRGEAEKLAHWRHEIAQHYYPHHCF
                     FYLDASAQDLPKTLSRKFSNDVNAWICQGVTCSQSIDSLNGLMAQI"
     gene            1977..2288
                     /locus_tag="PKL42_08490"
     CDS             1977..2288
                     /locus_tag="PKL42_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNU67151.1"
                     /translation="MKALLVTVAVAGSLMMAGQASANQALAQKSGCLACHSVDKKVLG
                     PSFKDVAAKYKGDKTSEAKLIEKVKKGGSGTWGPMPMPANSPQVKDEDIKTIVQWVLA
                     L"
     gene            complement(2372..3730)
                     /locus_tag="PKL42_08495"
     CDS             complement(2372..3730)
                     /locus_tag="PKL42_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HNU67152.1"
                     /translation="MSHTPHRSHWNNRWTFMLATAGSAIGLGNIWKLPYMIGVNGGSA
                     FVIIFLLCIFLVGIPLMMTEILLGRRAQKNPLDGMAALAHEANTTSHWKWLGGMGMIT
                     GLLILGFYSVIGGWVLRYIGASASGVFSNITAAQSTENFNQLLASPFSLLFWHTLFMI
                     MTMGVVARGVNSGLEKANNILIPALFCILLVLLGYSMSVGDMQTAYRFMFSLDFSKLS
                     PTVVLSALGHAFFSLSLGMGSVMVYGSYLQKNVSIARTTIYIALADTMLGLLVGLAIY
                     SLVFANQLEPSAGPGLIFQTLPIAFGHMPWGNFIATLFFMLVAFAAWTSAISLVEPAI
                     AWVVENTRIQRKTAAFFLGLTIWLLGVAVVLSFNEWKEVKIVFGLNIFESLDKLTSTI
                     LLPLGGLLMAIFAGFFMKKDHVQDELNLDTHKFKLWQFTNNYIAPLAIAAVFLYLLGF
                     IQ"
     gene            complement(3757..5085)
                     /locus_tag="PKL42_08500"
     CDS             complement(3757..5085)
                     /locus_tag="PKL42_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HNU67153.1"
                     /translation="MQALESLIKTLSDFVWGPPMLTLLVGTGLYLTILLKGMQFRTLP
                     LAFKLIFSKDHHHEGDISHFAALMTALAATVGIGNIVGVATAITLGGPGAVFWMWMTG
                     LVGMATKYSEAVLAIKYREKGPHGMRGGPMYYLAKGAGLPWLGTLFAVFTVCAAFGIG
                     NMTQANATAKIFEATFQIAPNITGWVLMILTGLVILGGIKSIGKFTSFLVPIMILAYI
                     GTALLVLILNIEKIPQAFGLIFYHAFNSSATIGGVVGATIAAAMRYGIARGVFSNESG
                     LGSAPIAAAAARTNDPVKQALVSMTQTFIDTLVVCTMTALVILTATSWTQGVSAAELT
                     SSSMAETLGNTGKVIVAIATALFAYSTLIGWSYYGEKAIEYLLGPRAIAVFRVIFTAA
                     VIIGANVSLTLVWDFSDLMNGMMAIPNLIGLLLLAKVVKAETNRYFSEKH"
BASE COUNT         1580 a         1163 c         1218 g         1362 t
ORIGIN      
        1 ttgaagaccc agtagtcgct gagctcttga atgcgcactt tatcaacatc aaggtagatc
       61 gagaagaacg gccggatatt gaccaaatct accaaactgc ccacagcatg ctttcacaaa
      121 aaagtggggg ctggccgctc actgtttttt taacacccaa tcaacaaccc tattttactg
      181 ggacgtattt tccaaaagct gcacgctacc agctgccagg ttttgctgac ttaattccac
      241 gtgttgcagc ctattatcac gagcatcgtg ctgatatcaa catacaaaca caacagctcg
      301 ccagtgtttt ggcgcgcact attccaatgg ctaatacaac cgtgacagcc gacgaacata
      361 ccattgagtt ggcctttgag cagctacgag agagctttga ttttgagcat ggcggttttg
      421 ggtcttcgcc taaatttcct aacccagcag atgtgacttt attgctgcat catgccaagt
      481 caggaaactc acaagcggag gccatggctt tgcaaatgct cactgcaatg gcatcgggtg
      541 gcatttatga ccatatcggc ggaggttttt gtcgatattc agtagatgag cgctggaata
      601 tcccgcattt tgaaaaaatg ctttatgaca acggccagtt attatgtctc tacaccgatg
      661 gctggcaaat tagtcagaat cctcgctatg aagtcgtggt tgaagaaacc gttgcgtggt
      721 tgatttgtga aatgcgacat gcgagtggcg ccttttactc ttctcaggat gctgattcat
      781 tagatgcaca cggccacgca gaagaaggcg cattttatgt atggcaacga gaagaagtta
      841 aagcattact gacgccagag gaatttattg cagcaagccg ctgttttggt tttgatcgcg
      901 ctgcaaattt tgaaaatccg cattcaggtg tacgtgcatg gcatccctat ttaaccatgg
      961 cgccggaaga cgatgagtac gttgccctga ataatgcgcg tactaagtta tttgaagcac
     1021 gtgaaaaacg ctcacgccct gcaagtgacg acaaaatttt aacgagctgg aacgcactgg
     1081 caatcaaagg cttggcgcga gcaggccgtg tatttaatcg aactgactgg atagcatttg
     1141 cgcaaaatgc agttgatttt gtacgtgaaa atatgtgggt gaatgctcag ttactcgcaa
     1201 ccagtaaaga tggcaaagca catttgaatg cgtatttgga tgattacgcc tttttacttg
     1261 atgcgctttt agagttgctg caggcagact atagaagcgt tgatatgcaa tttgctgaag
     1321 acattgccga tgtgctgatt gaaaactttg aagcggagga tggcggcttt tactttaccg
     1381 cacaccatca tgaagcgttg attcaccgtc ctaaacaagc ctatgacaat gcaaccccca
     1441 atggtaacgg cattgcggca ggcgcacttc agcgcttagg ccatttattg ggcgagccgc
     1501 gctatttaca aaaagcggaa cgtactttgc aggcgtttga tagcgtcgtt aaacgcaacc
     1561 cggcggcctg tgcaaattta agtgaggctt tgcgtgagta tttaacgccg ccgaccattg
     1621 tgattttacg cggtgaagct gaaaaactcg cgcactggcg gcatgaaatt gcgcaacatt
     1681 attatccgca ccactgtttt ttctacctag atgcaagtgc tcaagattta ccaaaaacat
     1741 tatctagaaa attttcaaat gatgtcaacg cgtggatatg ccaaggcgtt acctgctcac
     1801 agagtattga tagtctgaat gggctaatgg cgcaaatata gcttgttctt tggatgagtt
     1861 ggcttgtgag acaaagaaat tctgagtatc attactggtt gcgaaatttg caaaataaat
     1921 tttaggtgcg agttgttggg cgcgctgaat tataaatgtg ggcttaagga gataatatga
     1981 aagctttatt agttacagtt gcagtagcgg gttctttgat gatggcaggt caagcgtctg
     2041 caaatcaagc attagcacaa aaaagtggat gtttagcatg tcacagcgtt gataaaaaag
     2101 tattaggccc atcttttaaa gatgttgctg ctaaatacaa aggtgacaaa acttctgaag
     2161 ccaaactgat tgaaaaagtg aaaaaaggtg gtagcggtac ttggggtcct atgccaatgc
     2221 cagcaaacag cccacaagta aaagatgaag atatcaaaac aatcgtacaa tgggttttag
     2281 cgttgtgatt tgcttgaact cgtaatatta aaaagccagc attcatgctg gcttttttat
     2341 ttccaatttc gaaatataca aaatataaat tttattgaat aaaacctaat aagtataaaa
     2401 ataccgccgc aattgcaagt ggcgcaatgt agttattggt aaattgccat agcttaaatt
     2461 tatgagtatc taaattaagt tcgtcttgta catggtcttt tttcatgaag aatcctgcaa
     2521 aaatggccat caacagccca cctaacggta ataaaatggt agaagtcagc ttgtctaaac
     2581 tctcaaaaat attcaagcca aagacaattt tgacttcttt ccactcattg aatgaaagca
     2641 ctacggcaac gcctaatagc caaatagtga ggcctaaaaa gaatgcggcg gttttgcgtt
     2701 gaattcgcgt gttttcaaca acccatgcga tagcgggttc cactaaagag atggcggatg
     2761 tccacgcagc aaaagccact agcataaaaa ataaggtggc aatgaagttc ccccaaggca
     2821 tgtggccaaa cgcaatgggt agggtttgaa atatcaggcc gggccctgcg ctgggttcta
     2881 attggttagc aaacaccagt gaataaatag ccagacccac cagtaaccca agcatcgtgt
     2941 ctgccagtgc aatgtaaatc gttgttctgg caatagagac gtttttttgc aagtaagagc
     3001 cgtacaccat gactgagccc atgcctaaac tcaatgagaa aaaagcatgc ccaagggccg
     3061 acaaaaccac ggtgggcgag agtttcgaaa aatctaggct aaacataaat cggtaggctg
     3121 tttgcatatc acctacagac atgctgtaac ctaataacac caacaaaatg caaaacaggg
     3181 caggaatcaa tatattattg gctttttcga gcccggaatt taccccacgc gccacaacac
     3241 ccatagtcat gatcataaaa agggtatgcc aaaatagcag tgagaatggc gatgcgagta
     3301 actgattgaa attttcggtg gattgtgcgg cggtgatgtt gctaaaaacg ccacttgcag
     3361 aagcgccgat gtagcgcaac acccagccgc caatgacgct gtaaaagccg agaatgagca
     3421 agccggtgat catgcccatg ccacctaacc atttccaatg agatgtcgtg ttggcttcgt
     3481 gtgcgagtgc tgccatgcca tcgagcggat ttttttgggc gcgtcggccg agcaagattt
     3541 ctgtcatcat caacggaatg ccaaccagaa aaatacacag cagaaagata ataacaaacg
     3601 cactgccgcc attcacgcca atcatgtagg gcaatttcca gatgttgcct aagccaatcg
     3661 ccgagcctgc cgtggcaagc atgaaagtcc agcggttgtt ccaatggctg cgatgcggtg
     3721 tgtggctcat gatggcgtaa ttacatttaa attaaactaa tgtttttcgc taaaataacg
     3781 gtttgtttca gctttaacga ctttggctaa caataataaa ccaatcagat tggggattgc
     3841 catcatgcca ttcatcaggt ctgaaaaatc ccacactaag gtgaggctta cattggcacc
     3901 aataattaca gctgccgtaa aaattaccct aaacacagct attgcgcgtg gacctaataa
     3961 atattcaatg gctttttcac cgtaataact ccagccgatg agcgttgaat aagcaaataa
     4021 ggcggttgca atggccacaa tgacttttcc agtattgcct aaggtttcgg ccatacttga
     4081 gctggttaat tcagctgcgc taacaccttg cgtccaagac gtggcagtta aaatcactaa
     4141 agcggtcatg gtgcaaacca ctaaggtgtc aataaacgtt tgcgtcatgc tcactaatgc
     4201 ttgttttaca gggtcgttgg tacgcgctgc ggcggcagca ataggtgcag aaccaagccc
     4261 tgattcattt gaaaaaactc cacgtgcgat accgtaacgc atggctgccg caatcgttgc
     4321 gccaacaaca ccgcctatgg tggcgctcga attaaaggca tggtaaaaaa tcaacccaaa
     4381 agcctgcgga attttctcaa tattgaggat taatacaaga agcgctgtgc cgatatacgc
     4441 taaaatcatg ataggcacta aaaatgaagt aaatttacca atcgatttga tgccacctaa
     4501 aatcaccaga cccgtcaaaa tcatcagtac ccaacccgtt atattgggtg cgatttgaaa
     4561 ggtcgcttca aatatttttg cagtagcatt ggcttgcgtc atattgccaa taccaaatgc
     4621 ggcacatacc gtaaacaccg caaaaagcgt acctaaccaa ggcaatcccg cacctttggc
     4681 aaggtagtac atgggcccgc cacgcatgcc atgtggccct ttttcacggt atttaatggc
     4741 gagcacggct tcagaatact tggtggccat accaaccaag cccgtcatcc acatccagaa
     4801 tactgcgcca ggcccaccta aagtgatggc ggttgccacg ccaacaatgt tgcctatccc
     4861 cactgttgct gcaagcgcgg tcatcagcgc ggcaaagtgt gagatatcgc cttcatggtg
     4921 gtggtcttta ctgaaaatga gtttgaatgc caaaggcagt gtgcgaaatt gcatgccttt
     4981 tagaagaatg gtgagataaa gtccagtgcc taccagcagt gttaacatcg gcggccccca
     5041 cacaaagtca gaaagtgttt tgatgagaga ctctaaagct tgcatacgcc accagaaatg
     5101 gaagttgaat ggctagatta tagacataaa tcttgcgata tgtgaagttt gcctacttgg
     5161 gggcatgcct agcgcgtcta tattattaat cgtacgggca gaaattttag tacgcatttt
     5221 agacaataag cgcgtataaa aagagctgct tagggggtaa aattaacacc aacctttttc
     5281 gatgctctgg aagccttata aataaaggct tccagagcat tat
//
LOCUS       DAOQSI010000142         5315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_16012_length_5315_cov_4.005133, whole genome shotgun sequence.
ACCESSION   DAOQSI010000142 DAOQSI010000000
VERSION     DAOQSI010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16012_length_5315_cov_4.005133"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1893)
                     /locus_tag="PKL42_08505"
     CDS             complement(<1..1893)
                     /locus_tag="PKL42_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HNU67154.1"
                     /translation="MRKSKINQISLAVMLALTPLSIYAAGLGKLNTQSGLGEPLRAEI
                     EIFATPEELASLSAVIASEDAYAEQGITRLGVHSNIKVEVAKNASGAAVLKLRSSLPI
                     KDPYLDMLIQMDWASGRLQREYTVLLDPPEYKAADVAGVSTIESPSASTSDSANVAGQ
                     SSVKKSTTKKSKKAKKQAEPVAETQDEVIADVGGADEITTKSGDTLIAIARANQVEGV
                     SLDQMLAGLYNSNKDAFVSGNMNRLKVGKIIKVPSQESLTSISNRKARQVVKAHSSNW
                     NAYRNTMAGAVANLPTGEDDEQKQSSSGKITTAEDKAAAAQAGPKDVVKLSAGEKDAG
                     KAFDNKITALQEETTAQEKALKEAQDRAASLEAQIQDMQKLLALKNQSMADKQNAVTD
                     PVANAQPEVKPQPEVVPVPEVKPADASATQPAEAAPVAEVKPVEPAPAIEPAPAVKPA
                     PVVVPTPVAEPEAEPSFLDGLLGDNLPLIGGGLGLGLLGAGWLYLRNKRKKNLDSFER
                     GILTSGGLRANTVFGNTTNNTSTTDTSFLTDFAQSVDGGMIDTNDVDPIAEAEVYMAY
                     GRGAQAEEILKDAIQKEPKRYELHLKLLEIYAESKNNSAFEAIAGELYTTLGADDPTW
                     AKVARLG"
     gene            complement(2044..3156)
                     /gene="asd"
                     /locus_tag="PKL42_08510"
     CDS             complement(2044..3156)
                     /gene="asd"
                     /locus_tag="PKL42_08510"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNU67155.1"
                     /translation="MLKVGFVGWRGMVGSVLMQRMLEENDFALIEPQFFTTSQIGGKG
                     PNVGKDTPALLNAKDIDALKGMDAIVSCQGGDYTTEIFPQLRNSGWMGHWIDAASTLR
                     MEKNAVIILDPVNLHVIQDALKKGGNNWVGGNCTVSLMLMALNGLFKANLVDWATSMT
                     YQAASGAGAQNMRELLKQMGEAHFAASELLADPASAILDIDRTIAGTLRDDSFPTSHF
                     GVPLAGSLIPWIDKDLGNGQSKEEWKGGVETNKILGREASPIVIDGLCVRIGAMRCHS
                     QALTIKLTQDVPLDEINQMLSEANQWAKVIPNEREASMRELTPAAVTGTLSTPVGRLR
                     KLAMGGEYLSAFTVGDQLLWGAAEPLRRMLRILIEK"
     gene            complement(3192..3311)
                     /locus_tag="PKL42_08515"
     CDS             complement(3192..3311)
                     /locus_tag="PKL42_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HNU67156.1"
                     /translation="MFKHFVLILLMLAFAGCNTIHGVGKDIEKAGEAVQKSTN"
     gene            complement(3430..4509)
                     /gene="leuB"
                     /locus_tag="PKL42_08520"
     CDS             complement(3430..4509)
                     /gene="leuB"
                     /locus_tag="PKL42_08520"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNU67157.1"
                     /translation="MNIAVLPGDGIGPEIITQALRVLDVLKNEGMDMTFTEAPLGGQA
                     YDQYGHPYPEFTQNICRAADAVLLGAVGGPQYDNLDRPLRPERGLLGIRKDLGLFANL
                     RPAVLYPELANASTLKPEVVAGLDIMIVRELTGDVYFGQPRGMRTNENGEREGFNTMV
                     YSESEVRRIAHVAFGIAMKRRKNGQKAKLCSVDKANVLETTEFWKEIVIDVAKEYPEV
                     ELSHMYVDNAAMQLIRNPKQFDVMVTGNIFGDILSDEASMLTGSIGMLASASLNATKK
                     GLYEPSHGSAPDIAGKNLANPIATILSAAMMLRYTFDNEAAAQRIENAVKTVLAAGYR
                     TGDIFEEGMKRVSCSEMGDQIVAAM"
     gene            complement(4532..5170)
                     /gene="leuD"
                     /locus_tag="PKL42_08525"
     CDS             complement(4532..5170)
                     /gene="leuD"
                     /locus_tag="PKL42_08525"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNU67158.1"
                     /translation="MKAFTQLKGLACPIDRANIDTDAIIPKQFLKSIKRSGFGPYLFD
                     EWRYKDHGEPGMDCTNRPLNTDFVLNQPRYKGAQIMLARENFGCGSSREHAPWAIEDY
                     GFRVIIAPSYADIFFNNCYKNGMLPIVASHAMVDKLFNECQANEGYTLNVDLPSQTVT
                     LPSGETFTFDIHPTSKHNLLNGLDEIGLTLLHAEEIKAFEGKHKAAQPWLFA"
     gene            complement(5183..>5315)
                     /locus_tag="PKL42_08530"
     CDS             complement(5183..>5315)
                     /locus_tag="PKL42_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNU67159.1"
                     /translation="RCASTSNRNFEGRQGQGGRTHLVSPAMAVAAAVAGHFVDVRSL"
BASE COUNT         1377 a         1321 c         1063 g         1554 t
ORIGIN      
        1 gcctaatctg gccacttttg cccatgtcgg gtcatctgca cctaatgtgg tgtagagttc
       61 gccagcgatc gcttcaaatg cagagttgtt tttgctttcc gcataaattt ctaataactt
      121 caaatgcaat tcgtaacgtt taggctcttt ttggatagca tctttaagaa tttcttcagc
      181 ctgcgcaccg cgcccatacg ccatatagac ttcagcttca gcaatagggt caacgtcgtt
      241 cgtgtctatc atgccgccat caacactttg cgcaaaatcc gttaagaatg aagtatctgt
      301 agtgctggta ttattggttg tgttgccaaa tacggtattc gcacgcaaac cgcctgaagt
      361 taaaatgcca cgttcaaaac tgtccaagtt tttcttacgt ttattacgca aatataacca
      421 gcctgcaccg agcaagccca aacctaaacc tccaccgatc aacggcagat tatcgcccaa
      481 caaaccgtct aagaagctag gctctgcctc aggttcagca actggcgttg gcactactac
      541 tggcgccggt ttcacagcag gcgccggctc aatggcgggc gctggctcaa ctggtttaac
      601 ttcagctact ggcgctgctt ctgcaggctg cgtagcagaa gcatcggctg gcttaacttc
      661 tggcacgggt actacttcag gttgcggctt aacttctggt tgagcgttcg caactgggtc
      721 agtgactgca ttttgcttat cagccattga ctgatttttt aaagccagca atttttgcat
      781 gtcttgaatt tgtgcttcaa ggcttgcagc tcgatcttgc gcttctttta gcgctttttc
      841 ttgtgcggtt gtttcttctt gcaacgcagt aattttgttg tcaaaggctt taccagcatc
      901 tttttcacct gcagaaagct tcactacatc ttttggacca gcttgagcgg cagcggcttt
      961 atcctcagcc gttgtgattt ttcctgaaga agattgcttt tgctcatcat cctcaccggt
     1021 tggcaagttt gccacagcac ccgccatggt gttacggtaa gcattccagt ttgacgaatg
     1081 cgcttttacc acttgccttg ccttacgatt actaatagac gttaaactct cttggcttgg
     1141 cactttaatg attttgccaa cttttaagcg gttcatattg ccgctgacga aagcatcttt
     1201 attactgtta taaagacccg caagcatctg atctaaactg acaccttcaa cttggtttgc
     1261 tcgtgcgatt gcaataagtg tatcaccact ttttgtagtg atttcatcag caccacccac
     1321 atctgcaatg acttcatctt gcgtttcagc aacaggctca gcttgttttt tagctttttt
     1381 tgattttttg gttgttgatt tcttgactga agactgacca gctacattag cagaatcaga
     1441 tgttgacgca ctgggagact ctatcgtgct cacgcctgct acgtcagctg ctttatattc
     1501 tggtggatca agcaacacgg tgtattcacg ttgcaaacgc cctgaagccc agtccatttg
     1561 aatgagcata tctaaatatg ggtctttgat tggaaggctg gagcgtaatt ttaaaactgc
     1621 cgcgcctgat gcatttttgg caacttcaac tttaatatta ctgtgcaccc cgagacgggt
     1681 gatcccttgt tcagcataag catcttcaga ggcaattaca gcagataagc tggctagctc
     1741 ttcaggtgtt gcaaaaattt caatttccgc tcttaacggc tctcccaagc ccgattgagt
     1801 atttaattta cccaaccctg ccgcataaat tgaaagtggt gttaaagcca acatcaccgc
     1861 taaagatatt tgatttattt tagatttacg cacagtagtt ccttgatcag actataatcg
     1921 caaatattaa aataacatca aatagttagc gatgcaagct cagaatacac tttataataa
     1981 ttgttaataa taatgccgta tcttgttgtt gttgttttta caacaacaac aagataggaa
     2041 aggctatttc tctattaaaa tccgcaacat gcggcgtaat ggttcggctg caccccaaag
     2101 caactggtcg cccactgtga aggctgatag gtactctccg cccatggcaa gcttgcgcag
     2161 acgacccact ggcgtactca acgtgccagt cacagccgct ggcgtgagtt cacgcatact
     2221 ggcttcacgc tcgttaggaa taactttcgc ccattgattg gcttcactca acatctggtt
     2281 aatttcatcc agcggcacat cttgcgttaa tttaatggtt agcgcctgac tatggcaacg
     2341 catggcacca atacgtacac acaaaccatc aatcacaata ggactcgcct cacggcctaa
     2401 tatcttgttg gtctcaacgc cgcccttcca ctcttcttta ctttgtccat tccctaaatc
     2461 tttatctatc caaggaatca aactacccgc cagcggcaca ccaaagtgtg aagttggaaa
     2521 actgtcatca cgtaatgtgc cagcaatggt acggtcaata tctaaaatag cagatgccgg
     2581 gtcagccaac aactcgcttg cagcaaaatg tgcttcgccc atttgtttga gcaattcacg
     2641 catattttgt gcgcctgcac ctgaagcggc ttgataagtc atcgaagtcg cccagtcaac
     2701 caaattagct ttaaacaaac catttaatgc catgagcatg agtgaaactg tacagttccc
     2761 acccacccaa ttattgccgc cttttttcag cgcatcttgt atgacgtgca ggtttactgg
     2821 gtcgagaata atcaccgcat ttttttccat gcgtaatgtc gaggccgcgt caatccaatg
     2881 acccatccag ccagagttgc gcaattgcgg aaaaatttca gtggtatagt caccaccctg
     2941 acacgacaca attgcatcca tgcctttaag tgcatcgatg tcttttgcat tgagaagggc
     3001 cggtgtatct ttacctacat tcgggccttt accaccaatt tgcgatgtgg taaaaaactg
     3061 cggctcaatc agcgcaaaat cattttcttc taacatacgc tgcatgagca ctgagcccac
     3121 catgccacgc cagcctacaa atcctacttt tagcatactc aaacctatct taacggttta
     3181 ttaccatcaa tttaatttgt tgatttctga acagcttcac ccgctttttc aatatcttta
     3241 cccacgccgt gaatggtgtt gcaaccagca aaagctaaca tcagcaaaat caacacaaaa
     3301 tgtttaaaca tgattcggtc ctttttaata acgcattttt aataactaat tgttattcta
     3361 atggctttat gaagttttat atataaaagc gaccctttaa agcgtcgctt tttttataaa
     3421 acaaaagact tacattgctg ccacgatttg atcgcccatt tcagagcacg acacacgctt
     3481 catgccttcc tcaaagatgt caccagtacg gtaacccgct gccaatactg ttttcactgc
     3541 attttcaata cgttgcgctg ccgcctcgtt atcaaaagtg tagcgcaaca tcatggcagc
     3601 tgaaagaatg gttgcgattg gattagccaa gtttttgcct gcaatatcag gtgctgaacc
     3661 atgtgagggc tcgtacaacc cttttttggt tgcattgagt gaagcggatg ccaacatacc
     3721 aattgaacct gtcaacattg atgcttcatc ggacaagata tcgccaaaaa tattacctgt
     3781 caccatcacg tcaaattgtt ttgggttgcg aatcagttgc attgcagcgt tgtccacata
     3841 catgtggctc aattcaactt ctggatattc cttcgcaaca tcaatcacga tttctttcca
     3901 aaattcagtg gtttctagca cgttagcttt atccactgag cacaattttg ctttctgacc
     3961 atttttacgg cgcttcatgg cgataccaaa tgcaacgtgt gcaatgcgac gcacttctga
     4021 ctcgctatac accatggtgt taaagccttc gcgctcgcca ttttcgttgg tgcgcatgcc
     4081 gcgtggttga ccaaagtaca catcacccgt caattcgcgc acaatcataa tatccaaacc
     4141 tgccaccact tctggtttta gggttgaagc attggctagc tcaggataca aaacagctgg
     4201 acgcaagttt gcgaacaacc ctaaatcttt acgaatgcct aacaaaccgc gctcagggcg
     4261 caatggacgg tctaggttgt catattgtgg cccaccaacc gcgcctaaca ataccgcgtc
     4321 tgccgcacgg cagatgtttt gcgtgaactc tgggtaagga tgaccgtatt ggtcatacgc
     4381 ttgaccgccc aaaggcgctt ccgtgaaggt catgtccatg ccttcatttt tcaacacatc
     4441 taacacgcgc aatgcttgtg taataatttc tgggccgatg ccatcaccgg gtaatactgc
     4501 aatattcatt gtcatttcaa ttccttttaa tttaagcgaa caaccaaggt tgcgctgctt
     4561 tatgtttgcc ctcaaacgct ttaatctctt ctgcatgtaa taacgtcaaa ccaatttcgt
     4621 ccaaaccatt caacaagtta tgttttgagg ttggatgaat gtcaaacgtg aatgtttcgc
     4681 cagaaggcaa agtcacggtt tgtgaaggca agtctacgtt taaggtatac ccttcattgg
     4741 cttgacattc attgaacaat ttgtcgacca tggcatgact tgccacaatc ggcaacatac
     4801 cgtttttgta gcaattgtta aagaaaatat cggcgtaact tggcgcaata atcacgcgaa
     4861 agccgtaatc ctcaatcgcc caaggcgcat gctcacggct tgagccgcat ccaaagtttt
     4921 cacgcgccaa cataatttgt gcgcctttat aacgtggttg gttcaaaaca aagtctgtgt
     4981 tcaatggacg attcgtacag tccatgccag gttcaccgtg gtctttgtaa cgccactcat
     5041 caaacaaata agggccaaag ccagaacgtt taatactctt taaaaactgc tttggaataa
     5101 tcgcgtcagt gtcaatattg gcgcggtcta ttgggcaagc taagcctttt aattgggtaa
     5161 atgctttcat ttttgattcc ttttacaaac tgcgcacatc aacaaaatgg ccagcaactg
     5221 ccgcggcaac cgccattgct gggctaacta agtgtgtacg tccgccttgg ccttggcgac
     5281 cttcaaagtt gcggtttgaa gtagaagcac aacgc
//
LOCUS       DAOQSI010000143         5254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_16302_length_5254_cov_5.813618, whole genome shotgun sequence.
ACCESSION   DAOQSI010000143 DAOQSI010000000
VERSION     DAOQSI010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16302_length_5254_cov_5.813618"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..1036
                     /gene="speB"
                     /locus_tag="PKL42_08535"
     CDS             101..1036
                     /gene="speB"
                     /locus_tag="PKL42_08535"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HNU67160.1"
                     /translation="MDHAFAYLSTSGFLGLPIDKTPAHKRYAIAGVNWDGSCTNRPGA
                     RFGPSAIRHASQMLCDATHPLFEISPIEHLQDVGDLALPNTSLDAMRSALMPQVVDLI
                     KKQTCIWLGGDHSITLPLLRAYHAHYQQPLACIHFDAHCDTWTDHFGEPSGHGTWVYE
                     AITEGLVDPNCFVQIGIRSAGERTAREFVSDQGGKIYTARELRGKDGDALNTVIDEVR
                     ARMRKAGNPPLYLTFDIDALDPAFAPGTGTPEVGGLTTAQAMTLLEAWHDLNWVGMDL
                     CEVSPPYDHAELTSNAAATLVWTWICGKMANHEGN"
     gene            1156..1695
                     /locus_tag="PKL42_08540"
     CDS             1156..1695
                     /locus_tag="PKL42_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HNU67161.1"
                     /translation="MSEKTTTATQYKQGSIMTNILFGSRWLQLPLYLGLIVAQAVYVF
                     FFGVELVHLVSTAHKIKEADIMLIVLGLIDVVMISNLLIMVIVGGYETFVSRLNLEGH
                     PDEPDWLSHVNANLLKVKLATAIIGISSIHLLKTFINAENLTDKVLIWQTIIHMTFVF
                     SAVAIAYIDKLMSHSSAQH"
     gene            1714..3021
                     /locus_tag="PKL42_08545"
     CDS             1714..3021
                     /locus_tag="PKL42_08545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNU67162.1"
                     /translation="MLANDLKLTQHSYYVASANAQPDYPVLQGETQADVCVVGGGFAG
                     LSAAIELADRGYSVVVVEANTIGFGASGRNGGQLIAGLACEQAVIESALGFDAAKQVW
                     NMSLEALNLVRERVQRFNIDCDLTDGFLGVSVNPKKGASLRQWYDNMATRYQYDAIAE
                     WIAPQEIGRWIDSPRYFNGYYDKTGGHVHPLNYCLGLAKAAASLGVRIFQHSAVTHMQ
                     QGETVQLNTKIGSIKAQFVVLAGNMYLPEVSPQLAPSLGKRIMPVGTYIIGTEPIDAT
                     ITKTLIPSNAAVCDTNFILDYFRFSADHRMIYGGRVSYSALTPPNLTQSMQARMVETF
                     PQLKSTPVQFTWGGFVDITMSRAPDFGRLSPNVFYLQGFSGHGVALTGLAGKLVAEAI
                     SGQAERFDIFSKIQHHVFPGGKLLRMPALVLGMAWYKLRDALG"
     gene            3238..4863
                     /locus_tag="PKL42_08550"
     CDS             3238..4863
                     /locus_tag="PKL42_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNU67163.1"
                     /translation="MIAILEAKHAGLLSKAHWLNNILAGVIVGVVALPLAMAFAIASG
                     AKPEQGLYTAIVAGGLTSLFGGSRLQIAGPTGAFIVVLAGITAKYGIEGLQVATLMAG
                     VMLVVMGLTRLGGVIKYIPDPVIVGFTSGIGLIIFVGQWKDFFGLTAIIGVEHFHEKF
                     WHLVQAFPTFQLETTLLALLTVIVLLASPRVLKRIPSPLVAMVVATLVQWFFQFEQVA
                     TIGSAFGGIPQTLPHFQVPSITFSEVLKLIGPAFTIALLGAIESLLSAVVADGMSGTK
                     HDSNQELVGQGIANIFSPLFGGFASTGAIARTATNIRNGGNSPLAGLVHVATLVVIVL
                     LLAPLASHIPLCALSAILFVVAYNMSELHRFTHMVLTAPKADVAVLLITFLLTVFGDL
                     VIAVNIGVLLACLLFMKRMSEAVVITQQSQETLFQEVADVDFKLPENTVVFGMEGPFF
                     FGAAQHLESALESAHKHADVLVLRLDKVPFIDATGIQALWDLYDACKRHQTRLVLCQA
                     RPNVLNKLQLSGITGQMGKENVIDQLQQIRVAE"
BASE COUNT         1306 a         1115 c         1310 g         1523 t
ORIGIN      
        1 ttgagtgcgt gcatatcaaa gttgcagtgg ttaaaagttg gatgtatgag atattctacc
       61 tataatgttg tcagttgaca ttgttgtttt ggaactgaac atggaccacg catttgctta
      121 tttatcaacc agtggctttc ttgggttacc cattgacaaa acgcccgccc acaagcgcta
      181 tgcgattgcg ggggtgaatt gggatggcag ttgcaccaat cggccagggg cgcgttttgg
      241 ccccagtgcc atccgccatg ccagccaaat gctgtgtgat gcaacacatc ctttgtttga
      301 aatatctcca attgagcatt tgcaagatgt gggcgattta gctttaccaa atacgagcct
      361 agacgcgatg cgcagtgcat tgatgccgca agtcgttgac ctcatcaaaa agcaaacatg
      421 catctggctg ggaggtgacc attcaatcac gctgccgtta ctgcgcgcct atcatgccca
      481 ttatcagcaa cccttagcct gtattcattt tgatgcgcat tgcgatacgt ggacagatca
      541 ttttggcgaa ccttcagggc atggcacgtg ggtgtatgag gccattaccg aaggcttggt
      601 tgaccctaat tgctttgtgc agattggcat tcgctcagca ggagagcgca ctgcgcgcga
      661 gtttgtcagc gaccaaggcg gaaaaatcta taccgcgcgc gaattacgcg gaaaagatgg
      721 tgacgcgctt aatacagtga ttgatgaagt gcgtgcgcgt atgcgtaaag ccggcaatcc
      781 gcccttgtat ttaacttttg atattgatgc gctagaccca gcgtttgcgc ccggcaccgg
      841 cacgcctgaa gtgggcggct taacgactgc acaagccatg acgctattgg aagcttggca
      901 tgacctgaat tgggtaggca tggatttatg cgaagtttcg ccaccttatg accatgctga
      961 attaacttca aatgccgcag caacgctggt gtggacttgg atttgcggaa aaatggcaaa
     1021 tcatgaaggg aattaaaaaa gattaagttg gcaatttttt tgagtgaata aattcgtaca
     1081 agttaagtta tcatgcaagc tcaagctaaa atttgggcag taacctcaac atactataga
     1141 tagggactag gaaatatgag tgaaaaaaca acaaccgcca cacaatacaa gcaaggcagc
     1201 atcatgacca atattttgtt tggtagccgc tggctgcaac tgccgctata tttaggctta
     1261 atcgttgcgc aggcggtgta tgtgttcttt tttggtgtgg aacttgttca cttggtgtct
     1321 actgcacaca aaattaaaga agccgatatc atgttgattg tattaggttt aattgatgtg
     1381 gtgatgattt caaacttgct gattatggtg attgtggggg ggtatgaaac gtttgtgtcg
     1441 cgtctgaatc tggaaggcca ccctgatgag ccggattggc tttcacatgt gaatgccaat
     1501 ttgctgaaag tgaaactggc cacagctatt atcggcattt catccatcca tttactcaaa
     1561 acttttatca atgcagaaaa tttgacggat aaagtgctta tttggcaaac cattatccac
     1621 atgacctttg tattttcagc ggtggcaatt gcctatattg ataaattgat gtcgcattca
     1681 tctgcgcagc attaacggcc agccaccaaa cctatgctgg ccaatgattt aaaactcacg
     1741 caacattctt attacgttgc cagcgcaaat gcgcagccag attaccccgt tttacagggt
     1801 gaaacgcagg cagatgtatg cgtggtgggc ggtggttttg ccgggctatc agccgcgatt
     1861 gaattggctg accgtggtta ctcggtcgtg gtggtagagg ccaacaccat tggctttggc
     1921 gcaagcggcc gcaatggcgg gcaactcatt gccggtttgg cctgtgagca agcagtcatt
     1981 gaaagcgcgc tgggctttga tgccgccaaa caagtctgga atatgtcgtt agaggcgctc
     2041 aacttggtgc gcgagcgcgt tcaacgcttt aacattgatt gcgatttaac cgatggcttt
     2101 ttgggcgttt cagtcaaccc aaaaaaaggt gcatctctac gccagtggta cgacaacatg
     2161 gcaacgcgtt accagtatga tgctattgct gaatggatag caccgcaaga aattggccgt
     2221 tggatagaca gcccgcgcta tttcaacggc tattatgata aaactggtgg gcatgtgcat
     2281 ccccttaatt attgccttgg tttggcaaaa gctgccgcca gtttgggtgt gcgtattttt
     2341 cagcattctg ctgttacgca catgcaacag ggtgaaacag tgcaacttaa caccaaaatt
     2401 ggcagcatca aagctcagtt tgtggtgctg gcgggtaaca tgtatttgcc agaagtgtcg
     2461 ccacaattag cgccaagttt gggcaaacgc attatgccgg taggtactta tattattggc
     2521 accgagccga ttgacgcaac aatcaccaaa acgcttatcc ccagcaatgc ggccgtgtgt
     2581 gacaccaatt ttatattgga ttactttaga ttttctgctg accatcgaat gatttacggc
     2641 ggacgcgtta gctacagcgc actcacgccg cctaatttaa cgcaaagcat gcaagcgcgg
     2701 atggtggaga cttttccgca acttaaatct acgccagtgc aatttacctg gggcggcttt
     2761 gtggacatca ccatgagtcg tgcgccagac tttggaagat tgtcgccgaa tgtgttttat
     2821 ctgcaaggtt tttctggcca cggtgttgcg ctcaccgggc tggcaggtaa attagtggca
     2881 gaagccattt caggtcaagc agagcgcttc gatatttttt ccaaaattca gcatcatgtc
     2941 tttccaggtg gaaaattatt acgtatgccg gcactggtgc tcggcatggc gtggtacaaa
     3001 ttacgcgatg ccttaggctg attttttcag catccaaaac cgaatttaaa acgtctgttt
     3061 tcctccgagg attttttgtg cctctgtgag cctttattta taaggctcac agagcatcga
     3121 aaaaggttgc ttgaaaaatt cttgcatttt tcttagtttt ttttgaaagt attaagtgca
     3181 tcagctaggt catcaagcgt tatacttgtg acttcaattt tgtaaaatct gaacaccatg
     3241 atcgccattc tcgaagccaa acatgcgggc ctgctatcaa aagcgcattg gttgaataat
     3301 atattggcag gcgtcattgt tggtgtggtt gccttgcctt tggcaatggc ttttgccatt
     3361 gcatcagggg caaagcctga gcaagggctt tataccgcaa tcgttgctgg cggtttaact
     3421 tcattatttg gtggtagtcg tttacaaatc gcaggtccaa caggggcgtt tattgtagtt
     3481 ttagcaggca tcactgctaa atatggcatt gaaggtttac aagtggctac gctaatggca
     3541 ggcgtaatgc tggttgtaat ggggttgacg cgcttaggcg gcgtcatcaa gtacattcca
     3601 gatcctgtga ttgttggttt taccagcggg atagggctga tcatttttgt tgggcagtgg
     3661 aaagactttt ttggcttgac agccattatc ggcgttgagc attttcatga aaagttttgg
     3721 catctcgttc aggcatttcc aacgtttcag cttgaaacca ctttgctggc attactgact
     3781 gtaatcgtgt tattagcttc accgcgcgtc ttaaaacgta ttccatcccc tttagtggcg
     3841 atggtggtcg cgacacttgt tcaatggttt tttcagtttg aacaagttgc taccatcggc
     3901 agcgcttttg gcggcattcc acagactttg ccacattttc aggtaccttc catcactttt
     3961 tctgaggtgt taaagctgat tgggccagcg tttaccattg cacttttagg ggcaattgag
     4021 tcgctgttat cggctgtcgt ggcagatggc atgagtggca caaagcatga ctctaatcag
     4081 gagttggtgg ggcaagggat tgcgaatatt ttctcaccgt tatttggtgg atttgcctct
     4141 actggcgcca ttgcacgtac cgctaccaat attcgcaatg gtggtaacag cccgttagcg
     4201 ggtttggtac atgtggcaac actggtagta atcgtacttt tgttggcgcc actggcttcg
     4261 catattccgt tgtgcgcact ttcagcgata ttgtttgtgg tggcttataa catgagtgag
     4321 ttacaccgat ttacacacat ggtacttaca gcacccaaag ctgacgtggc cgtgctgctg
     4381 attaccttct tgctgacggt gtttggtgat ttagtgattg ctgtgaatat tggtgtatta
     4441 ctggcttgtc tactttttat gaagcgcatg tccgaagcgg tggtgattac acaacaatcg
     4501 caagaaactt tatttcagga agtagccgat gttgatttta agttgccaga aaataccgtg
     4561 gtgtttggca tggaaggtcc attctttttc ggggccgccc agcatttaga aagtgcttta
     4621 gaaagcgcac acaagcatgc agacgtccta gtgttaaggc tcgacaaagt accttttatc
     4681 gatgctacag gtattcaagc gttgtgggat ttgtatgatg cctgcaagcg tcatcaaacg
     4741 cgattggtgt tgtgccaggc gcgcccgaat gtgcttaata aactccaatt gtcaggcatt
     4801 actgggcaaa tgggtaaaga gaatgtcatt gaccaacttc agcagatacg tgtggctgaa
     4861 tagcgtttat ctgacaatac agtaaatcga tttatttccg gttattttcc tccgaggatt
     4921 ttttatgcct ctgcaagctt ttatttacaa ggcttccaga gcatcgaaaa aggtttcatt
     4981 taattttcat gctatttcac aattatcttt aaccctgagt agaaccagaa gccgctctta
     5041 ttgtcttgta ttgagttcaa tcataatatg aaacaaggaa agcgatgcag tttacaagtg
     5101 aggccattaa gttggccgaa aaaggcttaa ttccagatgc agcgattcgg ttaggcatac
     5161 gccaactttg caaaacacgg ctagatgaaa tttcagcagc caattgtgaa gccagtgcgc
     5221 aaattgaggc agattttatt gcagccatgc gtca
//
LOCUS       DAOQSI010000144         5240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_16381_length_5240_cov_4.403086, whole genome shotgun sequence.
ACCESSION   DAOQSI010000144 DAOQSI010000000
VERSION     DAOQSI010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16381_length_5240_cov_4.403086"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PKL42_08555"
     CDS             <1..352
                     /locus_tag="PKL42_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNU67164.1"
                     /translation="LMGIATKNSILLVEYAIVARRDLHMSRLDAILDACHKRARPIIM
                     TTIAMTAGMLPVALALGASDGSFRSPMAVSVIGGLLTSTLLSLLVIPVAYTLIDDVQQ
                     WIRKCLPTSKHEMT"
     gene            755..1744
                     /gene="rfaD"
                     /locus_tag="PKL42_08560"
     CDS             755..1744
                     /gene="rfaD"
                     /locus_tag="PKL42_08560"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HNU67165.1"
                     /translation="MIVITGGAGMIGSIIAWHLNTKHGRSDIVIVDSIQHPEQWQNLA
                     HRQYAQYLDKDQLMAWLQGRTDIEAIIHMGAISATTERDFNKLVGTNIHYSQNLWIWC
                     ANNNVPFFYASSAATYGDGNLGYNDASIDNLRPLNGYGYSKHFFDQWALRQVNENAPQ
                     PLSWAGFKFFNVYGPNEYHKERMASVAYHTFNQFSQTGTMKLFKGTKAGIEDGLQMRD
                     FVYVKDAASVVAYFLSNAHNTTPVKNGIYNIGTGQARSFKDLALAVMHGMGREPHITY
                     IDMPEDLQGKYQYFTEASMTKLKAAGYVAPFTSLEEGVKDYVQNYLLKPDAYC"
     gene            1776..2339
                     /locus_tag="PKL42_08565"
     CDS             1776..2339
                     /locus_tag="PKL42_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNU67166.1"
                     /translation="MKYVEYLKGEHAAHTAMFNALAPLFPTISDVGIAMQNTIQNGGK
                     ILIMGNGGSAADSQHIAAEIVGRFKKERKGMPAIALTTDTSILTSVGNDYGYDYIFAR
                     QIEALCRPEDLVIGITTSGNSANVVSAIEAAKAIGATTVGLTGGTGGKLKDLCDFNLV
                     MPSNVTARIQEAHIFVGHSLCEILESD"
     gene            2389..3846
                     /gene="rfaE1"
                     /locus_tag="PKL42_08570"
     CDS             2389..3846
                     /gene="rfaE1"
                     /locus_tag="PKL42_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HNU67167.1"
                     /translation="MHQKLIETVKQFGNTPLHALVIGDVMLDRYLMGEVSRISPEAPV
                     PIVLLKSQEDRIGGAANVAANLALLNIKTHMIGCVGHDNEANILLDLMAKIGIESSHI
                     LRSNHRPTIAKTRILGGHQQMMRLDQENSSIFTIDENAQLMATIDAQIRLKPAVVILS
                     DYAKGLLSTQTSQHIIAQCKLHNIPVLVDPKGKDYSKYSGATALTPNKKETAEACETT
                     INDPALIVKATQLKQNLNLAFLAVTRGEEGITLIDEKSQHLPAIAKQVFDVSGAGDTV
                     IATLAAGLIHQLNPVEALELANIAAAVVVGKVGTAPITQHDLLDALNTQQGNEQAHKV
                     CDLAQIMQKVEAWKKQRLKIVFTNGCFDLLHAGHVTYLEQAKKRGDKLILGLNTDRSV
                     SALKGPTRPVVNENDRARVLAALESVDAVILFDEETPLNLINTIQPHVIAKGSDYTAA
                     QVVGGKEVQSWGGEIALIDLVEGRSTSNIINKMNA"
     gene            3847..4872
                     /gene="waaF"
                     /locus_tag="PKL42_08575"
     CDS             3847..4872
                     /gene="waaF"
                     /locus_tag="PKL42_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HNU67168.1"
                     /translation="MTEKILILGPAWVGDMVLAQSLFKTLKTNRPNCTIDVAAPAWTL
                     PLLERMPEVSGKIALPFKHGELAFWQRLAFGKNLKNKGYTQSIILTNSLKSALLPWAA
                     GIKKRTGFLGEMRYGLVNDIRPLDKTKLKKTVERFVYLGLKKGESLPEKIHNPALVAD
                     KATALKVLGRFAHTPALSNRAKEQKILGLCPGAEYGEAKRWPAEYYAQVANYALDNGW
                     QVMLFGSDKDVPVTSQINQATQNRCLDLGGKTKLGEAIDLMSLCDTVISNDSGLMHVA
                     AALNKNLIAIFGSSDPYHTPPMHPKAMIEYLALECSPCFKRECPLGHLNCLKQIMPAT
                     IIKNLNT"
     gene            4874..>5240
                     /locus_tag="PKL42_08580"
     CDS             4874..>5240
                     /locus_tag="PKL42_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HNU67169.1"
                     /translation="MSHSKNIISLAYYLPQFHEIEENNRWWGKGFTEWSQLREAKTYF
                     KWQKIRMPVEPLGEYSLLNPHIMDLQSKIAKEYGINGFLVFDYWFGEGKTLLEKPMQL
                     VLDQQLNFDYCLCWANHTWY"
BASE COUNT         1586 a         1204 c         1133 g         1317 t
ORIGIN      
        1 gctcatgggc attgccacca aaaattcaat cttacttgtt gaatacgcca ttgttgccag
       61 acgcgacctg cacatgtcac gccttgatgc catattggac gcctgccata aacgcgcccg
      121 ccccatcatc atgaccacca ttgccatgac cgcaggcatg ttgcccgtgg ccttagcact
      181 tggcgcaagc gatggctcgt tccgtagccc catggcggtt tctgtgattg gtggcttact
      241 gacatccacc ttactaagct tgctggtcat tcctgttgcc tacaccttaa ttgacgatgt
      301 gcaacagtgg atacgaaaat gcttacccac ttccaaacat gaaatgacat aaaaaaatta
      361 aagcatttac acaaaataaa aagccgttaa aaagcctatt ttttgcgtta tttgtaccac
      421 aacctttttc gatgctctgg aagccttata aataaaggct tgcagggcag aaaaattttc
      481 tcgagagatt cttgccattc aattcattat tttttaatgc ttaatcagca catttgtaag
      541 cgacaaaaca ctacccttta gttttggaaa acaccgctat aaacgttata ctgttggctt
      601 taattgttga gttaaaaaac cctacggttt attaaaacag cctcatgaaa aatatcatct
      661 ttaatttttt agtgagcaca tttaacgcga ccgttaaatg gttaaaacgt aagtaaattt
      721 tacacttgct catcaccaat acaggccaaa cgctatgatt gtcattaccg gcggcgcagg
      781 catgataggc tctatcattg cttggcatct aaataccaaa cacgggcgca gtgatattgt
      841 cattgttgac agcattcaac accccgagca atggcaaaac cttgcgcacc gtcaatatgc
      901 gcaatactta gacaaagacc aactcatggc ttggctgcaa ggccgaaccg acattgaagc
      961 catcatccac atgggcgcca ttagcgccac aacagagcgc gatttcaata aactggtggg
     1021 taccaacatt cattacagcc aaaatttatg gatttggtgt gcgaacaaca acgtaccttt
     1081 cttttatgcc agctctgccg ccacttatgg cgatggcaat ttaggctata acgatgccag
     1141 cattgacaac ttacgtccgc tcaatggtta cggttactct aaacactttt ttgaccaatg
     1201 ggcattgcgc caagtaaatg aaaacgcgcc gcaaccgctc agttgggccg ggttcaaatt
     1261 ctttaacgtg tatggcccca acgaatacca caaagagcgc atggccagcg ttgcctatca
     1321 cacgtttaat cagtttagcc aaaccggcac catgaagtta tttaaaggta caaaagccgg
     1381 cattgaagac ggcttgcaaa tgcgcgattt tgtctacgtg aaagatgccg ccagtgtggt
     1441 tgcatacttt ttgagtaatg cgcacaacac aacaccagtg aaaaatggca tttacaacat
     1501 tggcacgggg caggcacgca gctttaaaga tttagccctc gcagtgatgc atggtatggg
     1561 tcgcgaaccg catattacct acattgatat gccagaagac ttgcaaggca aataccaata
     1621 ttttaccgag gcttcaatga ccaagctaaa agcggcgggc tatgttgcac cgtttactag
     1681 cttagaagaa ggtgtcaaag attacgtgca gaattaccta ttgaagccag atgcatattg
     1741 ttagcgcata ttgttaaaaa ggaccatcgc agcccatgaa atatgttgaa tacttaaaag
     1801 gcgagcacgc tgcccacacg gcgatgttca atgcccttgc gccacttttt ccaaccatta
     1861 gtgatgtagg cattgccatg caaaatacga ttcaaaatgg cggaaaaatc ctcattatgg
     1921 gcaatggcgg cagtgcggct gatagtcagc atattgcggc agaaattgtc ggacgtttta
     1981 aaaaagaacg caaaggtatg cctgcgattg cgctcactac tgacacgtca attttaacat
     2041 ctgttggcaa tgattacggt tacgattata ttttcgctcg ccaaattgag gccctttgcc
     2101 gcccagaaga cttggtgatt ggcatcacta cttcaggaaa cagtgcaaac gtggtcagtg
     2161 ctattgaggc tgcaaaggca atcggcgcca caaccgtcgg attaaccggt ggcactggtg
     2221 gcaaacttaa agatttatgc gattttaatt tggtcatgcc ttcaaatgtc accgcaagaa
     2281 ttcaagaagc gcatattttt gtggggcatt ctctttgtga aattttagaa agtgattaac
     2341 acacctttga ttcgatggtt aacaatcgca gggtaaaggc aatagttcat gcatcaaaaa
     2401 ctcattgaaa ccgttaagca atttggcaac acaccattac acgcactggt gattggcgat
     2461 gtgatgctgg accgctactt aatgggcgaa gtcagtcgca tctctcccga agcgcctgtg
     2521 ccgattgtcc tactaaagtc tcaagaagac cgcattggcg gcgcagccaa cgttgcggcc
     2581 aaccttgcgc tgctcaacat taaaacgcat atgattggct gcgtgggtca tgacaatgaa
     2641 gcaaatattc tgctggattt aatggcaaaa attggcattg aaagcagcca tattttacgt
     2701 tcaaatcacc gccctaccat cgcaaaaaca cgtattctcg gcgggcacca gcaaatgatg
     2761 cgcttagacc aagaaaatag cagcattttc accatcgatg aaaatgcgca actcatggca
     2821 acgattgatg cgcaaattcg cttaaaacca gccgtagtca ttttgtcgga ttatgcaaaa
     2881 gggctattaa gtacgcaaac cagtcagcac atcattgccc agtgcaaatt acacaacatt
     2941 cccgtgctgg ttgaccctaa aggcaaagat tacagcaaat acagtggtgc aactgcgctc
     3001 acgcctaaca aaaaagaaac tgccgaagcc tgtgaaacta ccatcaatga cccagcatta
     3061 atcgtaaagg caacgcaact taagcagaat ttaaacctag cgtttcttgc tgtcacacgt
     3121 ggtgaagaag gcattaccct cattgatgaa aaaagccagc atttacccgc aatagctaag
     3181 caagtatttg atgtttcagg tgctggcgat accgtaattg ccacgttggc agcgggctta
     3241 atacaccaac ttaacccggt agaagcctta gaattagcca acattgctgc tgccgtggtg
     3301 gtaggtaaag tgggcacagc acccatcact cagcatgatt tgctcgatgc actcaatacg
     3361 cagcaaggca atgagcaagc acataaggtt tgtgatttag cgcaaatcat gcaaaaagtt
     3421 gaagcttgga aaaagcagcg actcaaaatt gtgttcacca acggctgttt tgatttgctg
     3481 catgcaggcc atgtgactta tttagaacaa gcaaaaaaac ggggtgacaa gctcatttta
     3541 ggccttaaca ccgaccgttc agtcagtgca ctcaaagggc caacccgtcc tgtggtcaat
     3601 gaaaatgatc gtgcacgcgt gctggccgca cttgaatcag ttgacgcagt tattttattt
     3661 gatgaagaaa cgcccctaaa cctaatcaac accattcaac cgcacgttat cgccaaaggc
     3721 agtgattaca cagcagcaca agtcgtgggt ggcaaagagg tgcaaagttg gggtggagaa
     3781 attgcactga ttgatttagt tgaaggacgc agcacgagca atatcatcaa caagatgaac
     3841 gcgtaaatga ctgaaaaaat cctcattcta ggcccggcat gggtgggtga tatggtgctg
     3901 gcacaaagcc tatttaaaac actgaaaacc aacagaccca attgcaccat agatgtagcc
     3961 gcgcccgcat ggacactacc tttgcttgag cgtatgccag aggtatcggg taaaatcgca
     4021 ctgccgttta aacatggcga attagctttt tggcagcgct tggcttttgg caaaaactta
     4081 aaaaataaag gctatacaca aagtatcatt cttaccaatt cacttaaatc agcgctattg
     4141 ccttgggcag ctggtataaa aaaacgcacc ggttttttag gtgaaatgcg ttatggtttg
     4201 gtgaatgata ttcgcccatt agacaaaacc aaactaaaaa aaaccgttga gcgttttgtg
     4261 tatttaggct taaaaaaagg tgaaagcttg cctgaaaaaa tccacaatcc tgcgttggtg
     4321 gcagataaag ctaccgcttt gaaagtgtta ggcaggtttg cccacacccc agccctttcc
     4381 aatcgagcga aagagcaaaa aattctcgga ctttgcccag gcgcagaata cggcgaggcc
     4441 aagcgctggc cggctgagta ttatgcccaa gtggccaact acgcgctaga taacggctgg
     4501 caggtaatgc tgtttggctc agacaaagat gtgcctgtta ccagccaaat taaccaagcc
     4561 acacaaaatc gctgcctaga tttaggcggc aaaacaaaac tcggagaggc gattgacctc
     4621 atgtcacttt gcgataccgt catcagcaat gactctggtt taatgcacgt agccgcagca
     4681 ttgaataaaa acctaatcgc tatttttggt tcaagtgacc cctatcacac gccaccgatg
     4741 catccaaaag cgatgattga atatttagcg ctggaatgca gcccatgttt taaacgtgaa
     4801 tgcccattag ggcatttgaa ttgcctaaag caaattatgc ctgcaacaat tattaaaaat
     4861 ttaaatacat aagatgagtc actccaaaaa cattattagc ttggcctact acttgccaca
     4921 atttcatgaa attgaagaga ataatcgctg gtggggcaaa ggttttactg aatggtcaca
     4981 acttcgtgag gcgaaaacgt attttaaatg gcaaaaaata cgtatgccag ttgagcctct
     5041 cggtgaatat agcctactaa acccccacat aatggacctg caatcaaaaa ttgcaaaaga
     5101 gtatggaatt aatggcttct tggtttttga ctactggttt ggagaaggta aaactttact
     5161 cgaaaagccc atgcagctgg tgcttgacca acaacttaac tttgactatt gcttatgttg
     5221 ggctaatcac acctggtaca
//
LOCUS       DAOQSI010000145         5099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_17083_length_5099_cov_4.954798, whole genome shotgun sequence.
ACCESSION   DAOQSI010000145 DAOQSI010000000
VERSION     DAOQSI010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17083_length_5099_cov_4.954798"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..248)
                     /gene="ccoS"
                     /locus_tag="PKL42_08585"
     CDS             complement(111..248)
                     /gene="ccoS"
                     /locus_tag="PKL42_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HNU67170.1"
                     /translation="MDILFLLIPLSVVLVFAVLGGLWWAIYTGQFEEVEAEGERILHE
                     D"
     gene            complement(281..2608)
                     /locus_tag="PKL42_08590"
     CDS             complement(281..2608)
                     /locus_tag="PKL42_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNU67171.1"
                     /translation="MLTPALPQAQPTSTSLESDQRAWIALDDPAEWSGFSQALENEQQ
                     DWESRLAIEGMHCAACAFNVERSLKAVQGVSSVEVNATSGRARIVWSAALTKPSDWLS
                     AVSKAGYRALPAADAFAQDTRRKNQRLMLWRWLVAGFCMMQVMMYAYPAYVANAGEMT
                     PDMLNLLRWASWVLTLPVLLFSSGPFFSNALNDLKMRQISMDMPVALGILITFIVSTA
                     ATFEPNGWWGEEVYFDSLTMFVFFLLTGRWIELRMRDRTAGALDALMLRLPASVERQK
                     ADGSFERVAVRQLALGDVVRVLPGETFPADGTITLGSTSADEALLTGESRAVPKGLGT
                     EVIAGSYNLSAAVQVRIDKIGQTTRYAQIVALMERVSAEKPRLAILADRIARPFLITV
                     LFAAAASAAFWWQEDHGRALMAAVAVLIVTCPCALSLATPAAMLTVSGALAKSGVLVR
                     KMQALEALTTIDTVVFDKTGTLTHDRMEIGKISTAPHISQSAALAVASKMAQHSLHPV
                     SRALVQASQTQLADEAQIAVSNVQEISGFGLVADASLGKLKLGSAKFCEITDEVLQSL
                     QKPAESEQSSVVYLVANQVWLATFEVIEAVKEDAANAVSALQSLGLGVEVLSGDKLQS
                     VARIANLVGISQVNGGCTPQEKLQRLQFLQQQGRQVAMVGDGLNDGPVLAAAHVSIAM
                     GQAVPLAQSQSDFVIMSGELNMVPHLIHQAKRTMRIVKQNLIWAAVYNATCVPLAVAG
                     MLPAWLAGLGMALSSLLVVLNAARLANATKPITHP"
     gene            complement(3088..4464)
                     /locus_tag="PKL42_08595"
     CDS             complement(3088..4464)
                     /locus_tag="PKL42_08595"
                     /inference="COORDINATES: protein motif:HMM:NF020110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNU67172.1"
                     /translation="MNSLRLRLVLLLSAGLGLVWLVAAWFTHLESREEIDRLFDAQLA
                     QSAQVLLGTTRHEIHERIEHGEEEIAIKHEYEQKLAFQIWDEHDLLMRSTSAPTTVMG
                     SNREGFSEAIIDDAAWRVLTRWDSHHEFMIQVAEPLSGREALARHITLKMFMPTFFAL
                     PVLALLIWFGVGAGLKPLNTLKQEVKQRTANRLEPVAMTDVPEEVAPLVQSLNDLFAR
                     LAEAFDGERRFTADAAHELRTPLAALKIQAQVALRAKDDAERQKALENVLHGVDRATR
                     LVEQLLTLARVDPETALTHYHPLDLRALASAVLANMAPLAQAKKIELQLAEGGVCQVV
                     GDEDQLGLLLRNLLDNAIRYTPDGGQVSVKVYEGHVAETTVYESQTPKNTGTVLEITD
                     TGPGISPAEREQVQKRFYRIAGSGEEGSGLGLSIVQRIATLHGATLELQDRPQGHGLL
                     VKVIWPSK"
     gene            complement(4461..>5099)
                     /locus_tag="PKL42_08600"
     CDS             complement(4461..>5099)
                     /locus_tag="PKL42_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNU67173.1"
                     /translation="LGDGVRAGLKQAGFAVDWSQDGQAAKLALETEEYAVVVLDLGLP
                     KLSGTDLLKWLRATLSKVPVLVLTARDTVADRVSGLNAGADDYLIKPFDLDELIARIN
                     ALLRRSAGQVTQNLQHGNIILTPATHQVTQQGKSVELSAREFTLLHELLLHAGRVQSR
                     EQLEQHLYGWGEEVESNSIEVHIHHLRKKLGSDLIRTLRGVGYVIDQDASEK"
BASE COUNT         1337 a         1390 c         1095 g         1277 t
ORIGIN      
        1 tctcccgttt ttgtgctttt cactactttt gtaccagtta acattttaca tctttaatga
       61 gtcacatgcg gcattttgcc gctcaagact ttaaattcat ttgattcaaa tcaatcttcg
      121 tgcagaatgc gctcgccttc tgcttctacc tcttcaaact ggcctgtgta tattgcccac
      181 caaagtccac ccaataccgc aaacaccaat actaccgata acggaataag caaaaataga
      241 atgtccatga tattgatatt ccttataaaa ttaacgatgg ctatgggtgc gttatgggtt
      301 tagtcgcgtt cgccaaccgt gccgcattaa gtactaccag taacgaactc aaggccattc
      361 ctaagccagc aagccatgcg ggcaacatgc ccgccaccgc caatggaaca caggttgcgt
      421 tataaacagc ggcccatatc aagttttgct tcacaatgcg catggtgcgc tttgcctgat
      481 gaattaaatg cggcaccata tttaattctc cgctcattat aacaaaatct gattgcgatt
      541 gggctaatgg aaccgcttgt cccatcgcaa tcgacacatg ggctgcggcc agcactggcc
      601 catcatttaa gccgtcgccc accatcgcaa cctgacgacc ttgctgctgt aaaaactgca
      661 gccgttgcag tttttcttgt ggtgtacatc cgccgttcac ctgagaaatt ccaaccaaat
      721 ttgctatacg tgcgactgac tgtaatttat ccccagacaa cacctcaact cctaagccca
      781 aagattgcaa agcactgact gcattggcgg catcctcttt aacggcttct atcacttcaa
      841 aagttgccag ccaaacttgg ttagccacca agtacaccac agagctctgt tcgctttcag
      901 ctggcttttg tagactttgc aatacctcgt ctgtgatctc gcaaaacttt gcagaaccta
      961 atttaagctt acctaaactg gcatctgcaa ctaagccaaa gcctgaaatc tcttgtacgt
     1021 tactcaccgc aatttgcgcc tcatccgcta actgggtttg gcttgcctgc accagtgcgc
     1081 gcgacactgg atgcaacgaa tgttgcgcca ttttggaagc aaccgccagc gctgctgact
     1141 ggctgatatg cggggctgta gaaattttac cgatttccat gcggtcatgc gtgagcgtgc
     1201 ctgttttatc aaataccact gtatcaatag tcgtcagcgc ttctagtgcc tgcatttttc
     1261 tgaccagcac accacttttt gcaagcgcgc cagaaacagt caacatcgcc gcaggtgttg
     1321 ctaaagaaag tgcgcatggg caagtgacaa tcagcactgc cactgcggcc atgagcgcac
     1381 ggccatgatc ttcttgccac caaaaagcgg ccgaagcggc ggcggcaaac agcacggtaa
     1441 tcaggaatgg tcgggcgatg cggtccgcca gtatggctaa acgaggcttt tctgcagaaa
     1501 cacgttccat caacgccaca atctgcgcat agcgtgtggt ttgaccaatc ttatcaatgc
     1561 gcacctgcac ggcagccgat aagttataac tccccgcaat cacctcagtg cctaaccctt
     1621 taggcaccgc gcgcgactcc cctgttaata aagcttcatc cgcgctggta ctgccaagcg
     1681 taatcgtgcc atctgccgga aatgtttcgc cgggcaacac gcgcacgaca tcgcctaacg
     1741 ccagttgacg tacggccacg cgctcaaaac tgccatcggc tttttgtcgc tccacacttg
     1801 caggcagcct gagcatgagt gcatcgagcg cgcccgcagt gcgatcacgc atgcgtaatt
     1861 ctatccatcg acctgtgagc aagaaaaaca caaacatggt gagcgaatca aaatacactt
     1921 cttcacccca ccaaccattc ggctcaaatg tggctgccgt gctcacaata aacgtaatca
     1981 atatgcccaa cgccacgggc atatccatgc tgatttgccg catttttaaa tcattgagtg
     2041 cgttactaaa aaacggccct gaagaaaaca gcaataccgg aagcgtcagt acccaagagg
     2101 cccaacgcaa caaatttaac atatcaggtg tcatttcgcc ggcattggca acataggctg
     2161 ggtaggcata catcatgact tgcatcatgc aaaaacctgc cactaaccag cgccacagca
     2221 ttaaacgttg atttttacgg cgcgtatctt gtgcaaaagc atcagccgca ggcaaagcgc
     2281 gatagcccgc tttgctgact gcacttagcc agtcggaagg tttggtgagc gctgccgacc
     2341 acacaatgcg tgcgcggcca ctggtggcat tcacttcaac actgctgacg ccctgcactg
     2401 ccttgagtga tctttcaaca ttaaatgcac aagccgcaca atgcatgccc tcaatggcta
     2461 agcgcgattc ccagtcttgc tgttcgtttt caagggcttg actaaaaccg gaccactcag
     2521 ccgggtcatc cagcgcaatc catgcgcgct ggtctgattc cagcgacgtg gatgtaggtt
     2581 gggcttgagg taatgctggc gttaacatgt cgatatttta ccactgagta atttgctggt
     2641 gagcataaca taggtacttt acccaacatt aacacatatc aatgcagaat agtttaagtg
     2701 tactcaagaa aatctaacgc agctacgcat gagttttgtt gcaaaaatcc ttgaaaggaa
     2761 acctttgcac gaggcagatt taagctgaaa aaatcacaat ctcgtcattc ccgcgtaggc
     2821 gggaatccag ttaattttca agattactga ttgtaattaa atgctttaac catttaccaa
     2881 gcctagattc ccgcctacgc ggaaatgaca gatttagaca attttcagat aaaatttcaa
     2941 catattttga gttgtgcaaa ggtctctaaa atttcacatt gctgaagata gcaaaactgc
     3001 cgagaaaatt ttactgctct gcaagccttt atttataagg ctcacagagc atcaaaaaag
     3061 gttggtatga aaaaagtgaa aaaaacctta ctttgaaggc caaatgactt tgaccagcaa
     3121 gccatggcct tgcggcctat cttgaagttc cagcgtcgcg ccatgcaacg tggcaatgcg
     3181 ttgcacaatt gacagcccta aaccgctgcc ttcttcgcca ctgccggcaa ttcgataaaa
     3241 acgcttttgt acctgttcgc gctctgccgg tgaaatacct ggcccagtat ctgtaatttc
     3301 aagtaccgtg ccagtatttt tgggcgtttg gctttcatac accgtagttt cagccacatg
     3361 gccttcataa actttcacgc ttacttggcc gccatctggt gtgtagcgaa tggcattgtc
     3421 caataaattg cgtagcaaca acccgagttg atcttcatcg ccgaccacct gacatacgcc
     3481 accctcggcc aattgcagct caattttttt tgcctgcgcg agcggcgcca tattagccaa
     3541 gactgcactc gccaacgcac gcaaatcgag cggatgatag tgcgtaagtg cagtttctgg
     3601 gtccactcta gccaaggtta acaattgctc aaccagccga gttgcacggt ctacaccgtg
     3661 taatacattt tctagtgcct tttggcgttc tgcatcatct tttgcacgca gggcaacttg
     3721 agcttgaatt ttgagcgctg cgagcggcgt gcgtaattca tgtgcggcat ctgccgtaaa
     3781 acggcgctcg ccatcaaagg cttctgctaa tcgggcaaat aaatcattta acgactgaac
     3841 cagaggcgcc acttcttcag gcacgtccgt cattgccact ggttcaaggc gatttgcggt
     3901 tctttgtttg acttcttgtt taagcgtatt taatggtttt aagcctgcgc ccacaccaaa
     3961 ccaaatgagc aatgccagca cgggcaacgc aaaaaatgta ggcatgaaca tcttcagcgt
     4021 aatatgccgc gccaacgcct cgcgaccaga taaaggctca gccacttgta tcataaattc
     4081 atggtgcgaa tcccaacgcg tgagcacccg ccaagcagcg tcatcaatga tggcttcgct
     4141 aaagccttcc cgattgctgc ccatcaccgt ggtgggtgca gaagttgagc gcatcaataa
     4201 atcatgctca tcccaaatct gaaaagccaa cttttgctca tactcgtgtt tgatggcaat
     4261 ttcttcttca ccatgctcaa tgcgctcgtg tatttcatgc cgcgtagtgc ctaatagcac
     4321 ttgggcagat tgcgcgagct gtgcatcaaa taagcggtca atttcttcgc gcgattccaa
     4381 atgcgtaaac catgcggcaa ccaaccacac aaggccaagg cctgcggaca acaataaaac
     4441 caagcgtaac cgtagtgaat tcatttttcg gacgcatcct gatcaatcac atatccgacg
     4501 ccacgtaagg tgcgaatcaa atcactgccg agtttttttc gtaagtggtg aatatgcact
     4561 tcaatcgagt tgctttcgac ttcttcaccc cagccgtaca aatgttgctc taattgctcg
     4621 cgcgattgca cgcgacccgc gtgcaacaga agttcgtgca gcaaagtgaa ttctcgcgct
     4681 gatagctcga cacttttacc ttgctgcgtc acttgatgcg tagcgggcgt cagaataatg
     4741 ttgccatgct gtaagttttg cgtgacttgg cctgcactgc ggcgtaacag cgcattgata
     4801 cgggcaatca gctcgtctaa atcaaacggt ttaattaagt aatcatcggc acccgcattt
     4861 aagccagaca cacgatcagc aacagtatca cgcgcagtaa gcactaacac cggcacttta
     4921 gaaagcgttg cacgcaacca tttaagcaaa tctgtaccgc ttaattttgg caggcctaaa
     4981 tcaagcacta ccaccgcata ttcttcagtt tctagtgcca actttgctgc ctggccatct
     5041 tgcgaccaat ccaccgcaaa gccggcctgc tttaaacccg cccgcacacc atcgcccaa
//
LOCUS       DAOQSI010000146         5042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_17371_length_5042_cov_6.469220, whole genome shotgun sequence.
ACCESSION   DAOQSI010000146 DAOQSI010000000
VERSION     DAOQSI010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5042
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17371_length_5042_cov_6.469220"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(464..988)
                     /locus_tag="PKL42_08605"
     CDS             complement(464..988)
                     /locus_tag="PKL42_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNU67174.1"
                     /translation="MKKFLALLALAILPLTVAAHGPSPQKVEKTITIKAPPAKVWAIV
                     KDFGNMQAWHPAIASDKLEKKGEETFRTLTLKSGGEIYEKLRSADDKDMKLKYEIVSG
                     VAPVADYNSFMTVTSGPGAGESTVTWVGRFYRTYKLNPPIPAGQDDETAVKFVTDVYD
                     AGLAGLKKAAENSK"
     gene            complement(1161..2108)
                     /locus_tag="PKL42_08610"
     CDS             complement(1161..2108)
                     /locus_tag="PKL42_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HNU67175.1"
                     /translation="MRILILVLLLQLSACTAIFNKGSTAYITNQGDNTVSVIDILQGK
                     VTQTIAVGKAPVGVAVSKSLKRAFVTNVESGDISVIDTISNQVIETIRIGGSPVGIAI
                     ASDNKTLYVADWFSDRILAINTASYNQRREVIVAKAPAGLQISPDNKWLYIAARDTNE
                     IAVIDTATLTISKRIAVGEHPFGLALSKDGSTLYTVNVYENTVSLINTKTWQQTKIKV
                     GEHPYCAVSSPDSKTLYVTNTQDDTVSVIDVASKKPITTIEVGMTPEGISYDAASHQV
                     LVASWGENQVSVIDARTNKLKDHIKTGDKSRAFGQFILP"
     gene            2193..2708
                     /locus_tag="PKL42_08615"
     CDS             2193..2708
                     /locus_tag="PKL42_08615"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HNU67176.1"
                     /translation="MLDRDGFRPNVGIILCNAQNQVFWGKRIREHAWQFPQGGIKYGE
                     SPEQAMYRELMEEVGLRPEHVQILGRTRDWLRYEVPSSWVKREWRGSYRGQKQIWYLL
                     RMVGRDTDVSLRASEHPEFDAWRWSDYWVPLEDVIEFKREVYKLALNELSAHLHHKVR
                     PSSHKNTAKQV"
     gene            complement(2772..3611)
                     /gene="asd"
                     /locus_tag="PKL42_08620"
     CDS             complement(2772..3611)
                     /gene="asd"
                     /locus_tag="PKL42_08620"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183960.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HNU67177.1"
                     /translation="MPFSIVLQYLLPKQAITMLAGKFATLQGGQLTTAVIGWFVKRYN
                     VNMAEAANPDIASYTSFNEFFTRPLKNNARPLAQAEFVCPVDGTISQFGNIEQDQIFQ
                     AKGHNYSTLALLAGQQPLAAKFQNGHFACLYLSPKDYHRIHMPCDGTLKSMAYVPGDL
                     FSVNPTTAANVPNLFARNERVVCEFESEKHGTFIMVLVGATIVGSMATVWHGVVNPPR
                     PKNTRTWSYIDQNIQLKQGDEMGRFLLGSTVVMLFEKDALRFNTDWQPARAIRLGEMM
                     SAT"
     gene            complement(3679..4695)
                     /gene="ilvC"
                     /locus_tag="PKL42_08625"
     CDS             complement(3679..4695)
                     /gene="ilvC"
                     /locus_tag="PKL42_08625"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNU67178.1"
                     /translation="MKVYYDKDADLSIIKSKKVTIVGYGSQGHAHAANLKDSGATVTI
                     GLRQGGSSWAKAVAAGHNTLEIADAVKEADVVMVLLPDETMAAIFDAEIAPNLKKGAA
                     LAFAHGFNIHYNQIVPRADLDVIMIAPKGPGHTVRSEYLKGGGVPSLIAVYQDKSGKA
                     KDIALSYAAANGGTKGGVIETDFREETETDLFGEQAVLCGGAVELVKAGFETLVDAGY
                     APEMAYFECLHELKLIVDLMYEGGIANMNYSISNNAEYGEYVTGPKVINDESRYAMQE
                     ALANIQNGNYAKQFILEGRTNYPEMTARRRLNAAHPIEQVGGQLRAMMPWIAKNKLVD
                     QSKN"
     gene            complement(4746..>5042)
                     /gene="ilvN"
                     /locus_tag="PKL42_08630"
     CDS             complement(4746..>5042)
                     /gene="ilvN"
                     /locus_tag="PKL42_08630"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNU67179.1"
                     /translation="KLVDVVKVLDLNDGKFIERELMLIKVKATSTHREEMKRMCDIFR
                     GRIIDVADGSFTIELTGSGTKLDAFIESLDKTAILETVRTGASGIGRGDRILKI"
BASE COUNT         1231 a         1173 c         1206 g         1432 t
ORIGIN      
        1 taacacacta gattaagtag tcgataccag tgcttgaata agtattagca acgggtgtgc
       61 caacttggaa taacattagt taacttattg ttttttaata tataaaataa tttacatcaa
      121 aaagtagcta ctgaattgag caattaaagg cataaaaaaa cgggttttac cccgtttttt
      181 caagcattgg attgacaaaa agtgtcatac cataccaaac ttgtcacctt ctggccgtgg
      241 cttgatgcag tttgcgtcac cctttacgag cgggtattgt gggctatgct gttaaataaa
      301 aaaggatcgc aaatttgcga tccttttttt gtaggaggcg cggcctatgc cgatagtaat
      361 ttttttactc gcgaagaggc tttgctccta cagatttacc aaaacttcca ccatgatttt
      421 ttgccagagg atgtattagt cactgcgcca gttgcctggc tagttacttt gagttttcgg
      481 ctgccttttt caaaccagct aaaccggcat cataaacgtc agtcacaaat ttcacggcag
      541 tttcgtcatc ctgacctgct ggaataggtg ggtttaactt gtaggtgcgg taaaaacggc
      601 ctacccatgt cacggtggat tcgccagcac ccgggccaga ggttactgtc atgaatgagt
      661 tgtaatcagc taccggtgct acgccgctca cgatttcgta tttcagtttc atgtctttgt
      721 catcggcgct acgcagtttt tcgtaaatct cgccaccaga tttcaatgtc agcgttctga
      781 aggtttcttc accttttttc tctaatttat cactggcaat ggctgggtgc caagcttgca
      841 tgttgccaaa atcttttact atggcccaca cttttgcagg cggcgccttg atggtgatgg
      901 ttttttcaac tttttgtggg ctagggccgt gcgctgcaac ggtcagcggt aaaattgcca
      961 atgcaagcaa tgctaaaaac tttttcattc aatatctcca gtaaataaac caagcaaaaa
     1021 ataaatacaa cgcgttatta taaatcagat gagccaataa gcccacggtt tgagtaacgc
     1081 ttgatatggt gtagcgttga cgggcttggt aggagcgaat ttattcgcta ctacaaaaca
     1141 aactgcgttt ctatatctta ttagggcaat ataaactgtc cgaatgcacg gcttttatca
     1201 cccgttttaa tatggtcttt aagtttgttg gttcgcgcat caatcacgct gacctggttt
     1261 tcaccccagc tggcgaccag cacctgatgg ctcgctgcat cgtaactgat gccttccggc
     1321 gtcatgccga cctcaatggt tgtaatgggt tttttacttg ctacatcaat cacagaaacc
     1381 gtgtcatcct gcgtgttggt gacatacagt gttttgctgt ctgggctgga aaccgcgcaa
     1441 taagggtgtt cccccacttt tattttagtt tgctgccagg ttttagtatt gataagtgaa
     1501 acggtatttt cgtacacgtt cacggtataa agtgtgctgc catctttgct tagcgcaagc
     1561 ccaaacggat gctcgccgac ggcaatgcgt ttggaaatcg tcagcgtggc cgtatcaatc
     1621 accgcgattt cattagtgtc gcgcgctgca atgtataacc atttgttatc agggcttatt
     1681 tgcaagcccg caggcgcttt ggcgacaatg acttctcggc gctgattata gctggctgtg
     1741 tttatggcaa gtatgcggtc gctaaaccag tcggcgacat acagcgtttt gttatcggac
     1801 gcgatggcga tacccacagg cgagccgccg attctgatgg tttcaattac ctggttgcta
     1861 atggtgtcaa taacggaaat gtcgccgctt tctacgttgg taacaaacgc gcgctttagc
     1921 gattttgata ctgcgacacc cacgggcgct ttaccaacgg cgatggtttg agtgactttg
     1981 ccttggagta tatcaatgac tgaaaccgtg ttgtcgccct ggttggtgat ataagcggtg
     2041 ctgcctttat tgaatattgc cgtgcacgcg ctgagctgca acaataaaac caagataagt
     2101 attctcataa attacattct aaattcaaat gcctgctttc catagcgctt aaaatatgaa
     2161 aaaatggcgt aaacaattta ggtagttgca atatgttaga tcgtgacgga tttcgcccga
     2221 atgtggggat tattctgtgt aacgctcaaa atcaggtgtt ttggggcaag cgtattcgag
     2281 aacacgcttg gcagtttcca caaggcggca ttaaatacgg cgaaagccca gagcaggcta
     2341 tgtaccgtga gctgatggag gaagttggct tacggccgga gcatgtgcaa attttaggtc
     2401 gcacgcgtga ctggctgcgc tacgaggtgc catccagctg ggtcaagcgc gagtggcgcg
     2461 gcagttacag gggtcagaag cagatttggt atttgctgcg catggtaggc cgcgatacgg
     2521 atgtttcgct gcgtgcgagt gaacatcctg aattcgacgc atggcgctgg agcgattact
     2581 gggtgccgct cgaggatgtg atagagttca agcgtgaagt atataaactg gcgctaaacg
     2641 agttgtcggc gcacctgcat cacaaagtta ggccgagctc gcataaaaat acggccaagc
     2701 aagtataacg gccattcaaa taaaaaaacc tacccagaaa ttgtatgggg caggtttttt
     2761 atttgtccag attatgtagc gctcatcatt tcacccagcc tgattgcgcg cgctggctgc
     2821 cagtctgtat taaatcgcag cgcatctttt tcaaacagca tcactacggt agagcccaac
     2881 aaaaaacgtc ccatttcatc gccctgtttt agctggatat tctggtcaat ataagaccag
     2941 gtgcgtgtgt tttttgggcg tggtgggtta accaccccat gccacaccgt cgccatactg
     3001 ccgacgatgg ttgcgcctac cagcaccatg atgaaagtgc catgcttttc tgattcaaac
     3061 tcgcatacca cacgctcatt ccgtgcgaat aaattgggca cattggcggc ggtagtcgga
     3121 ttcaccgaga ataaatcgcc tggcacatag gccatgcttt ttaatgtgcc atcgcaaggc
     3181 atgtggatgc ggtggtagtc tttcggactt aaatacaggc atgcaaaatg gccattttga
     3241 aacttggcgg ctaatggctg ctggcctgca agcagtgcca atgttgaata gttgtgacct
     3301 tttgcctgaa aaatctgatc ctgctcgata ttgccaaact ggctgatggt gccatcgact
     3361 ggacaaacaa actcagcttg cgccagaggg cgggcgttat tttttagggg ccgcgtaaaa
     3421 aactcgttaa agcttgtata gcttgcaatg tcaggattag cggcttcggc catgttgacg
     3481 ttgtaacgtt tcacaaacca gccaatgacg gcggtggtca gttgcccccc ctgcaaggtg
     3541 gcgaatttgc ccgcaagcat ggtaatggct tgttttggca gtaaatattg taagacgata
     3601 gagaatggca attgaaatcc ttcaataaaa aagggcaaaa cgtcttgcgt cttgccctct
     3661 aaaatgaata gtttgcgatt agtttttaga ctgatcgacc agtttgtttt tcgcaatcca
     3721 aggcatcatg gcacgcaact ggccacctac ctgttcaatc ggatgcgctg cattcaggcg
     3781 gcgacgcgca gtcatttcag ggtaattagt gcgaccttct aaaatgaact gtttggcgta
     3841 attgccattt tgaatgttgg ctaacgcttc ttgcatggcg taacgtgatt catcgttaat
     3901 cactttaggg ccggttacgt attcgccgta ttccgcgttg tttgagatcg agtagttcat
     3961 gttagcgatg ccgccttcgt acatcaaatc aacaatcaat ttcagctcgt gcaggcactc
     4021 aaagtatgcc atttcaggtg cgtagccagc gtctaccaat gtctcgaagc cagctttaac
     4081 cagttctacc gcgccgccgc ataatacagc ctgttcgccg aacaagtcgg tttcggtttc
     4141 ttcgcggaag tcagtttcaa tcacgccacc tttcgtgccg ccgttggctg ctgcatatga
     4201 aagcgcgatg tctttggctt tgccagattt gtcctgatag acagcaatca atgaaggtac
     4261 gccgccgcct ttaaggtatt ctgaacgcac ggtgtggccg gggcctttcg gtgcaatcat
     4321 gatcacatct aagtccgcac gaggcacgat ctggttgtag tgaatgttaa agccgtgcgc
     4381 aaaagccaat gcagcacctt ttttcaggtt gggtgcaatc tcggcatcaa aaatagcggc
     4441 catggtttcg tctggcaata acaccatcac aacatcagcc tctttcacgg catccgcaat
     4501 ttccagcgtg ttatgacctg ccgcaaccgc tttagcccat gagctgccgc cctgacgtaa
     4561 gccaatagtc acagttgcgc cgctgtcttt cagattggcg gcgtgcgcgt ggccttgcga
     4621 gccatagccc acgatggtta ctttcttgct tttgatgatg cttaaatcag cgtctttgtc
     4681 gtagtaaact ttcattatga tttgcctttc gatacaaata cttaattatt ttggatattg
     4741 tttggttata ttttcaaaat gcggtcgccc cggccgatgc cggatgcccc tgttcgcaca
     4801 gtttctagaa ttgcggtttt gtccaagctc tcgataaaag catccagctt ggtgcctgag
     4861 ccggtaagct caatggtaaa acttccgtcc gccacatcga taatgcggcc acgaaaaatg
     4921 tcacacatgc gtttcatttc ttcacgatgt gtgctggttg ctttaacctt gatcagcatc
     4981 aattcacgct cgataaattt gccgtcgttc aaatccagca cttttaccac gtcaaccagc
     5041 tt
//
LOCUS       DAOQSI010000147         5011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_17518_length_5011_cov_4.864407, whole genome shotgun sequence.
ACCESSION   DAOQSI010000147 DAOQSI010000000
VERSION     DAOQSI010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17518_length_5011_cov_4.864407"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..260
                     /locus_tag="PKL42_08635"
     tRNA            184..260
                     /locus_tag="PKL42_08635"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:218..220,aa:Pro,seq:ggg)
     gene            493..1854
                     /locus_tag="PKL42_08640"
     CDS             493..1854
                     /locus_tag="PKL42_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNU67180.1"
                     /translation="MPLNLDPRYATDAASERVNRLIYQSLVDFDQHSRPTPSLASWLV
                     LSNREFQFTLKAKRSAFNTPTKIRSTFLNARDVKATYDSFTNLADSPHAAEFSNIQEI
                     VVLNDDTLIFKLKKDDANFPTKLLIGILPAALVEKNHDFSHHPVGSGSIQLLDWGNKL
                     TLQRVKDGQRISLLEVKDPTVRVLKLLHGEVDLLQGELPSELIQHLESKSEITLKNSV
                     GANFSYLGLNMQDPILQSLKVRQAIAHAIDRETIIAKVMTRQTRLASAILPPEHYTNH
                     KSALPAYDYNPKRARQLLLEAGVQLPLKLVYKTSTDAQRVRLATIMQAQMAEAGIDLE
                     IRSLDWGTFFADVKQGNFQLYGLTWVGIKTPEIYRKVFGSDSFPPHGFNRGRFSDASL
                     DQLLVVENWPAVTKRIHAQLPYIPLWYEGQFAAMRNNIIHFTPKTDGNWDDLAKITYR
                     KAH"
     gene            1950..2426
                     /locus_tag="PKL42_08645"
     CDS             1950..2426
                     /locus_tag="PKL42_08645"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNU67181.1"
                     /translation="MNIDISIARQTLTLLDSAGTTKAVYCISTATNGVGCEKNSGCTP
                     LGTHIIRAKIGMGAPLNAVFVGRRLTGEICTPELMAQFPNRDWILTRILWLSGTEIGK
                     NRLGNVDSMQRYIYIHGTPDSEPMDEPHSHGCIRMRSADVIALFEQVKVGTRVLIN"
     gene            2557..3477
                     /locus_tag="PKL42_08650"
     CDS             2557..3477
                     /locus_tag="PKL42_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNU67182.1"
                     /translation="MISIFKQISSFSTVIFGVLLLTFLLIHLVPGDPVEVMLGESAST
                     ADRDMLRANLGLNQPVVTQFSRYLIKLAHGDFGQSIHTKTPIIEMLKTRYPATVKLAL
                     LSLLIGISIGIPLGIFAALKANHWQDIVVTLVSVRLSAMPAFWLGPMLMLIFAVWLGW
                     LPVSGMESNTSIILPALTLGFGLSAILTRMTRTSLLEVLNDDYIRTARAKGLTEKTVI
                     LRHALRAALLPIITIVGLQMGSLLAGTVITETIFSWDGIGRLLVESIEKRDYPVTQAC
                     VLVVALSYVLVNFVTDVLYRMADPRVKFAS"
     gene            3483..4274
                     /locus_tag="PKL42_08655"
     CDS             3483..4274
                     /locus_tag="PKL42_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNU67183.1"
                     /translation="MKKFAITVLSFWLIAVMLGRLFGLNPNSIDLNAILSTPSAAYWL
                     GADDLGRSILARLLRGVEVSFVVATVVTVVTMTIGVLVGLIAGFYGGKIDQFLMKITD
                     IFLAFPGILLAIAFAAVLGPGLVNLTIALSMTGWVSYARLTRGQTLALRYRQHVLAAE
                     SLGATVPRLIFRHILPLLASILVVEATYSLASVMIAEASLSFLGLGIQAPNASWGAML
                     RDGVRYMLVAPHYVLIVGLSLMSLILAINLGGDYLRDKLDVRTEK"
     gene            4304..4795
                     /locus_tag="PKL42_08660"
     CDS             4304..4795
                     /locus_tag="PKL42_08660"
                     /inference="COORDINATES: protein motif:HMM:NF022400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HNU67184.1"
                     /translation="MTQITELASVEDEATAVFVGKNYQYYQKKWATAAVKKSPLSWNW
                     AAFFLGIIWLVYRKMYSLAAIVFAILVLDVVIETYFPLPESLGRAVTWAVAALFGWFG
                     NIWYKSHVDKKVKAINAAFAPEQVNIELAKQGGVNAPAAWTIGIILLILLAAAVWVIL
                     SEV"
     gene            4812..>5011
                     /locus_tag="PKL42_08665"
     CDS             4812..>5011
                     /locus_tag="PKL42_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNU67185.1"
                     /translation="MSLGPVMIDVVGTALSADDIKRLQHPLVGGVILFKRNYENNAQL
                     KALTASIHAVRQPTLLIAVDHEG"
BASE COUNT         1292 a          982 c         1234 g         1503 t
ORIGIN      
        1 ataatgaagc gcaagcaagt aatgattttg atttagcctc attaaaagca gagttgcttg
       61 aaatattagc tttgcttcgc ccttcaatgc aagcaacctc ataattttag ctggccagct
      121 aaaattagcc tccctaattg cggtataata cagcctgtgt tgaagtgttg ctcaacatcg
      181 gttcggggcg tagcgcagcc tggtagcgta catgcatggg gtgcatgggg tcgcaagttc
      241 gaatcttgcc gtcccgacca aataaataag ccattcttta tagattggct ttttctttat
      301 ctgagggtga attttgttta gtttcgggtc tacgttcaaa gtagaagtgc ttttcacatg
      361 cgaggtttaa cactgttaaa accgactttg ttggcaattg tgttggtgat atgcttttca
      421 gtagccaatg gcagtgcaac atcttctgag ccaacatcgc atggtcgcga cattatattt
      481 gctattggac aaatgcccct taacttagac ccgcgctatg cgacagatgc tgcttcagag
      541 cgcgtgaaca gacttattta tcaaagcttg gtcgattttg atcaacattc aaggcccaca
      601 cctagcttgg cttcttggtt ggtcttgagt aatagagagt ttcaattcac attaaaagca
      661 aaacgttcag cttttaatac cccaactaaa atccgctcaa catttttaaa tgcccgagat
      721 gtgaaggcga cctatgactc atttaccaat ttagctgact cacctcatgc tgcggaattt
      781 tcaaatattc aagaaattgt tgttctcaat gacgacactt taatttttaa gttaaaaaaa
      841 gacgatgcta attttcccac taaacttttg ataggtattt tacctgcagc tttagtagaa
      901 aagaatcacg acttttcgca tcaccctgtc ggtagtggct cgatacaact gcttgattgg
      961 gggaataaac tcactttaca gcgggtaaaa gatggtcagc gaatcagttt gctagaagtg
     1021 aaggacccaa cagtccgtgt gttgaaactg ctgcatggtg aagtggattt attgcaagga
     1081 gagttgccgt ccgaattaat tcaacatttg gagagcaagt ctgaaatcac gctaaaaaat
     1141 agtgtgggcg cgaatttttc atatttaggt ttaaatatgc aagatccgat tttacagagt
     1201 ttaaaagtac ggcaagccat agcgcatgcg attgaccgcg aaaccatcat tgcaaaagtc
     1261 atgacgcgcc aaacacggct agccagcgcg atattgccac ccgagcatta cacaaatcac
     1321 aagagtgcat tgcctgctta tgattacaac cccaaacggg cacggcagtt gttactggaa
     1381 gcaggcgttc agttaccact caaattagta tataaaacat caacagatgc ccagcgagtc
     1441 cgcttggcaa ctatcatgca agcacaaatg gctgaggctg gaattgattt ggaaattcgt
     1501 agccttgatt ggggtacgtt ttttgcggat gtaaaacagg ggaattttca gttatatgga
     1561 ctgacatggg ttgggattaa aacgccggag atatatcgta aagtattcgg ttctgatagc
     1621 tttccccccc atggatttaa tcgtggtaga ttttctgatg catcgttaga ccagttgctg
     1681 gtagttgaaa actggccggc agtgaccaaa agaattcatg cacaattacc atatattcca
     1741 ctttggtatg aaggacagtt tgcagctatg cgtaataata taatccattt cacgcctaaa
     1801 acagatggga attgggatga tcttgctaaa attacttatc gtaaggcgca ctaatttaat
     1861 aaatactgcc attttgcaat gtggcgaggt gctaaaaaag tgctttgtta acgcttcatt
     1921 aagatgcgcc taagtcagaa atgaatgaaa tgaatattga tatttctata gctcgccaaa
     1981 cgcttaccct gctagatagc gcaggtacaa cgaaggctgt ttattgtata tcaacggcta
     2041 cgaatggcgt gggatgtgaa aaaaatagtg gttgtacccc acttggtaca catattatcc
     2101 gtgcaaaaat tggtatgggt gcgcctttaa acgccgtgtt tgttgggcgc agattaacgg
     2161 gtgaaatttg cacgcctgag ttaatggcgc aatttccaaa ccgtgattgg attttaaccc
     2221 gtattttgtg gctaagcggc actgaaattg gcaaaaatag gctgggcaat gtcgatagta
     2281 tgcagcgtta tatttacatt cacggcacac cagatagcga gccgatggat gagccacact
     2341 cacatggctg tattcgtatg cgcagtgcag atgttattgc gttatttgag caggttaaag
     2401 tgggcacgcg cgtgctgatt aactaagtga tatttgtctg atttatcatg taaaaattga
     2461 cgtaaccatt ttagcaccgc tgcaagcctt attctatgag gcttgcaggg cattaaattt
     2521 ttctcgaggg ttttgtaggt ttataatagt gtgcgaatga tctcaatatt taaacaaatt
     2581 tccagctttt ccactgttat ttttggcgtg ctgttgctca cttttttgct catacattta
     2641 gtgccgggcg accccgttga agtcatgttg ggcgagtctg ctagcacggc tgaccgcgat
     2701 atgttgcggg caaatttggg cttaaatcag ccggtcgtga cgcaatttag tcgctatttg
     2761 attaaattag cgcatggcga ctttgggcaa tccatccata caaaaacgcc aataattgag
     2821 atgcttaaaa cgcgttatcc agcaacggta aagcttgcct tgctgtcatt gttgattggt
     2881 atcagtattg gtataccgct tggtattttt gcggcactta aggccaatca ctggcaggat
     2941 attgtggtta cgctcgtcag cgtgcggctt tcagccatgc ctgcgttttg gctggggccg
     3001 atgttgatgt tgatatttgc cgtgtggtta ggttggttgc ctgtgagcgg tatggaatca
     3061 aacacttcta ttattttgcc tgcgctcacg ctgggttttg gcttaagcgc aattcttacg
     3121 cgcatgacgc gaaccagcct gttagaggta ctgaatgatg actacatccg tactgcacgc
     3181 gctaaaggcc tcacagaaaa aactgtcatt ttgcgccatg cgttgcgcgc tgccttatta
     3241 cccattatta caattgttgg cctgcagatg ggcagtttgt tagcgggtac ggtgattacc
     3301 gaaaccattt tcagttggga tggcatcgga agacttttag ttgaaagtat cgaaaagcgc
     3361 gattacccag tgacgcaggc ttgcgtgctg gtagtggcgt taagttatgt gttggtgaat
     3421 tttgttacag atgttttata tcgcatggca gacccgcgcg tgaagtttgc gagctaaaca
     3481 gtatgaagaa atttgccatc actgtgttgt cattttggct tatcgctgtg atgcttggcc
     3541 gactatttgg gcttaatccg aacagtattg atttaaatgc tattttgagt acgccaagtg
     3601 ctgcttattg gttaggggca gatgatttgg gccgcagtat tttggcgcga ttgttgcggg
     3661 gcgttgaagt ttcatttgtg gtggctactg tggtgacggt agtcaccatg accattggtg
     3721 tgttggtagg tttaattgcc ggtttttatg gtggcaaaat tgaccagttt ttgatgaaaa
     3781 tcacggatat ttttttggca ttccccggca ttttgttggc gattgcgttt gctgccgtgt
     3841 tagggcctgg cttggtgaac ctcaccattg cgctcagcat gacaggctgg gtaagctatg
     3901 ccagactcac acgcgggcag accctagcat tgcgctaccg tcagcatgtg ctggctgccg
     3961 agtcgttggg cgctactgtg ccacgcctga tttttcggca tattttgcca ttgctggctt
     4021 ccatcttagt ggttgaagcc acgtatagtt tagccagcgt gatgattgcc gaggcttctt
     4081 tgtcatttct cggtttaggc atacaggcac caaacgcgag ctggggcgcg atgctacgtg
     4141 acggtgtgcg ctatatgcta gtcgcaccgc actatgtgct gattgtgggc ttgagtttga
     4201 tgagtttaat tttagccatc aatttaggtg gcgattattt gcgtgataaa ctcgacgtta
     4261 gaacagaaaa ataactcact aaaattttta tagggagaac atattgacac agatcactga
     4321 attggcaagc gttgaggatg aagccacagc cgtatttgtg ggcaaaaatt accagtatta
     4381 tcaaaagaaa tgggcaacgg cagccgtaaa aaaatcaccc ttgtcatgga attgggccgc
     4441 tttctttttg ggaattattt ggttggtcta tcgaaaaatg tatagtctag ccgctattgt
     4501 ttttgcgatt ttggtacttg acgtggtgat tgaaacttat ttccccctcc cggaatcact
     4561 aggacgagcg gtgacttggg cagtggcggc tttattcggc tggtttggca atatttggta
     4621 taaaagtcac gtagataaaa aggtcaaggc tatcaatgct gcctttgcgc cagagcaagt
     4681 caatattgag ttggcaaagc aaggtggcgt aaatgcgcct gcagcttgga cgattggcat
     4741 tattttgttg atattacttg cggcagctgt ttgggtgatt ttatctgagg tttaaggaat
     4801 gaaggattag tatgtcatta ggtccagtga tgattgatgt tgtaggcaca gcgctcagcg
     4861 ctgacgacat caagcgtttg caacatccgc tcgtgggcgg ggtgattttg tttaagcgta
     4921 attatgaaaa taacgcacaa ttaaaagcac tcacagccag cattcatgcg gtgcgtcagc
     4981 caacattatt gattgcggta gatcatgaag g
//
LOCUS       DAOQSI010000148         4949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_17823_length_4949_cov_4.786473, whole genome shotgun sequence.
ACCESSION   DAOQSI010000148 DAOQSI010000000
VERSION     DAOQSI010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17823_length_4949_cov_4.786473"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PKL42_08670"
     CDS             <1..231
                     /locus_tag="PKL42_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNU67186.1"
                     /translation="ASLNANPLNGALSMASFALGTSVSLFVGPWLWLKLKNGSQWVTE
                     NTSMRLAGLLLSAAAGWAIWMDVMHNTKVWCN"
     gene            complement(489..1199)
                     /locus_tag="PKL42_08675"
     CDS             complement(489..1199)
                     /locus_tag="PKL42_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HNU67187.1"
                     /translation="MKKSLLVLALAATFAPALAYAEAGDIVVRLRATNINPSEDSKLG
                     QQTNKAYGAGTAELLYGSANANLQVDGNTIPELDLSYYVTKNIALELILALGTKHDVS
                     TSSKGAGLNKKGLGEVNLLPPTLTAQWHFMPDTTFDPYVGAGLSYVRAMDNGLTADTI
                     LGRKPIRIDRNSWGPAIQAGFDVNLQDGWLVNFDIKKIWFDTDVKIKVPELAAGYKKI
                     DSLDIDPLVVSVGIGKKF"
     gene            complement(1588..3099)
                     /locus_tag="PKL42_08680"
     CDS             complement(1588..3099)
                     /locus_tag="PKL42_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNU67188.1"
                     /translation="MAKQPAQFEVQPVLGLYNSGKLAEAEVAAKKLLSHYPETFILYQ
                     VLGLAQDGLNKFDEAVDSYQKALALQPNTPDLHFNLGIALTNVGRQEEAVKSYAQAIK
                     LRPTFFEAYANLGTILQKQGKLEEAIKNYQHALSIHEDPRGHFNLGTALRDEGKLDEA
                     ITHFKQAIAMFPNYADAHNYLGECYRDQGNMEDAVKKYFDTLALNKNHPGANYNMGEF
                     LYLAGRYDEAVGYLERSKLDDWQERALYCTYKAEHFDAFKTKLENIVQTGAKHNAPFL
                     QTLSTHYATNFGVEDTYNFCKNGFDFVYQKSIPELAEPDSQFLKDLLRDIDNTAIEVR
                     AQAMIINGKQSAGNLFKRPEASFRKLGEIIKQEFLNYKNKFAGADCELIKSFPNELEF
                     TSSWYVRLRRGGFVDRHIHEVGWISGAVYLVLPKDKKDPDEGCFEYGLHGDNYPLQHE
                     NFPTGIARPKVGDVVMFPSSLFHRTIPFTGNEERICIAFDLKPEGEIFRRSNY"
     gene            complement(3167..4474)
                     /locus_tag="PKL42_08685"
     CDS             complement(3167..4474)
                     /locus_tag="PKL42_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HNU67189.1"
                     /translation="MQEPNNKPKFSASLQQAFRKPLNAYARQRYSTPLFVIHEMLNAF
                     RRHNTFGLSASLSFYAMLALIPMVLLMFFTISHLVFSSDYAVVKLAIIMGTLAPNFSS
                     TIMVEVYNAAQHKAVWGAAGLFVLLWAVTPLASAMRSTFYTIATMVESPSFIRRKLKD
                     LIAVVGMLLLFFLFTFVGLMLEKVIAFLAANNQLFRHEFVTTIASLLLTTLLIAAFYF
                     AFFPVRLYLKHILIGALFTAVLWLLMRPAFTLFLTLNASYGSVFGSMKNLFISITWLY
                     MNFIVFLLGTELIAILRKKDVLMLKNLFVIPEKNNAESQSNYLAKLMQRFGKTFAKDE
                     VIFKQGDIARNLHYLVNGQVAIVRDGKAIRTLSSGDYFGEMALLSNSPTTADAIVTST
                     TADVVVIYPDSIETLLLEEPKVAMLFLRQMATRLQTLNAGHAS"
     gene            complement(4499..>4949)
                     /locus_tag="PKL42_08690"
     CDS             complement(4499..>4949)
                     /locus_tag="PKL42_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HNU67190.1"
                     /translation="ENKRHNPIMAYEQLIFSDDINISIPAAIRWNAFEGNIMTLLPRA
                     ATTNNDAIDGEIELARARIQIHYIQHHCFVGDRDLLAEAKQKLAHIPTQILQGRYDMV
                     CPPITAWELSRAMPHAEFQLIEDAGHSAMDASLTSALVAATEKFKAL"
BASE COUNT         1374 a         1038 c         1084 g         1453 t
ORIGIN      
        1 gcctcactca atgccaaccc tctaaacggc gcccttagca tggcaagctt tgcgttaggc
       61 accagcgttt cgctatttgt gggcccgtgg ttatggctaa aacttaaaaa cggtagccag
      121 tgggtcacag aaaataccag tatgcgcttg gctggcttgt tattaagtgc cgcagccggc
      181 tgggcaattt ggatggatgt gatgcacaat accaaagtgt ggtgtaatta gtcagtgctg
      241 ttacaggagc gcaattcatt gcgcgaattt aatacatgga ggcatcttat tcattttcag
      301 catacccgct agatgttgtg ctttatatat agcggtttcg cgcaatgaat tgcgctccta
      361 caaaaaacat ttcaccaagc aataggggta gcgcaaaaaa agcccctgta aaatatcaca
      421 ggggcgaagg tggatttctt gattgggttc atatcagaac ccaaaacttt atgtgctagc
      481 ggttgatatt agaatttttt accgatacca acactcacta ccaatgggtc gatgtctaag
      541 ctgtcaattt tcttgtaacc agctgccaac tcaggcactt taattttaac gtctgtatca
      601 aaccagattt ttttgatatc aaagtttacc aaccagccgt cttgcaaatt cacatcaaaa
      661 cctgcctgaa tcgctggacc ccagctgtta cggtcaatac ggattggttt tctacctaaa
      721 attgtgtccg ctgttaagcc gttatccatt gcacgaacat agctcagacc tgcgcctaca
      781 taaggatcaa atgttgtgtc aggcataaag tgccattgtg cagttaatgt aggtggtaat
      841 aaattaactt cgcctagtcc ttttttgttt aatcctgcac ctttgcttga tgtgcttaca
      901 tcatgttttg taccaagcgc taaaatcaac tctagcgcaa tgtttttagt cacgtagtaa
      961 gacaaatcca actctggaat tgtgttgcca tctacttgta aatttgcatt tgcactgccg
     1021 tataacaatt ctgcagtacc agcaccgtag gctttatttg tttgttgacc taatttacta
     1081 tcttctgatg ggttgatatt ggttgcacgt aaacgcacga caatgtcgcc tgcttcagcg
     1141 taggctaaag ctggtgcaaa agtggctgct aatgctaaaa ctaataatga ctttttcatt
     1201 tttaaacttc ctttgattga aataaacaaa tataaatttg atagacgaat gttactgagg
     1261 gtgatacttg ggtgatttga tacatatcaa acaacagcca gctaaacggg tatgtatgac
     1321 gaatgttgtg tttttgcaac acatattaaa tagtcaaaaa ttcacatgaa gttcaggccg
     1381 aaatataaaa tattacgccg aattttacaa gtgaaaataa agcgattttt gccaacaaat
     1441 agcccgcaac ctttttgact gccctgagag ccttataaat aaaggctcgc agggcatctt
     1501 ttttctctcg gagctttttt gcgctttttt aactaaaaaa acggggtaaa aattgccccc
     1561 gttttgctga tttaaatgga tgcgaggcta gtagtttgag cgcctaaaaa tttcgccttc
     1621 aggttttaag tcaaaggcga tacaaatgcg ttcttcattg cctgtaaatg gaatggtgcg
     1681 gtgaaacaaa gaggaaggga acatgacaac gtcccctact ttaggccttg caatgccggt
     1741 aggaaaattt tcatgttgca acggatagtt gtcgccatgc aaaccgtatt caaagcagcc
     1801 ttcatcgggg tcttttttgt ctttgggtag cactaaatag accgcgccgc taatccagcc
     1861 cacttcgtga atgtgcctat ctacaaagcc gccacgccgc aagcgcacat accaagagct
     1921 ggtaaattct aattcgttgg gaaatgattt aatcagttcg caatctgccc cggcaaattt
     1981 atttttataa tttaaaaatt cttgtttgat gatttcgccc agttttctaa aagaggcttc
     2041 aggacgctta aataaattgc ccgctgactg cttgccgttg ataatcatgg cttgtgcgcg
     2101 cacttcaatg gcggtgttgt caatgtcgcg cagtaaatct tttaaaaatt ggctgtcagg
     2161 ctcggcaagt tcgggaatgc ttttttgata aacaaaatca aagccgtttt tgcaaaaatt
     2221 gtacgtgtct tctaccccaa agttggtggc gtagtgcgtt gatagcgttt gtagaaaagg
     2281 tgcattgtgt ttagcgcctg tttgcacaat attttcaagc ttggttttaa aggcgtcaaa
     2341 atgttcagcc ttgtaggtgc aatacagcgc gcgctcttgc caatcgtcta attttgagcg
     2401 ctctaaataa cccacggcct catcatagcg ccccgccaga tataaaaatt cgcccatgtt
     2461 gtagttggcg ccggggtggt ttttgttaag tgcgagcgtg tcgaagtatt ttttaaccgc
     2521 atcttccata ttgccttggt cgcggtagca ttcgcccaag taattatgtg cgtcggcata
     2581 atttggaaac atcgcaatgg cttgcttgaa atgcgtaatg gcttcatcga gtttgccttc
     2641 gtcgcgcagc gccgttccta aattaaaatg cccgcgcggg tcttcatgaa tgctaagtgc
     2701 gtgttggtaa tttttaatgg cttcttctaa tttaccttgt ttttgcaaga ttgtgcctaa
     2761 gttggcatag gcttcaaaaa aggtggggcg tagcttaatg gcctgtgcat agcttttaac
     2821 agcttcttct tgccgcccaa cgttggtgag cgcaatgcct aagttaaagt gcaggtcggg
     2881 cgtgttgggt tgcaaggcca gcgctttttg atagctatca acggcttcgt caaatttatt
     2941 taagccgtcc tgtgcaagac ctagtacctg atacagaata aaggtttcgg ggtagtggct
     3001 gagtagcttt tttgcggcca cttcagcttc agcgagcttg cctgagttat ataagcctaa
     3061 aacgggttgt acttcaaact gtgcgggttg ttttgccatt attcattatc actttgttga
     3121 gcggttacat cattttagaa taaaactatt taacttgcat ggtctgttaa cttgcatggc
     3181 ctgcgttgag cgtttgcagg cgcgtggcca tttggcgcaa aaacagcatt gctacttttg
     3241 gctcttctaa taataaggtt tcaatactgt cagggtaaat caccaccaca tctgcagttg
     3301 ttgatgtgac aattgcgtca gccgtagtgg gggaatttga cagcaacgcc atttcgccaa
     3361 agtaatcgcc cgatgataat gtgcgaattg ctttgccatc acgcacaatt gccacctgcc
     3421 cgttcactaa ataatgcaga ttgcgtgcaa tatcgccttg tttaaaaatc acttcatcct
     3481 ttgcaaacgt tttaccaaaa cgctgcatca attttgccaa atagttagat tgtgattcag
     3541 cgttattttt ctcagggatt acaaaaagat tttttaacat caatacatct ttttttcgca
     3601 agatggctat cagttctgtg cctagtaaaa acacaataaa attcatgtaa agccaggtaa
     3661 tagaaataaa aaggtttttc atgctaccaa aaaccgagcc gtaagatgca tttagcgtta
     3721 aaaatagcgt aaacgctggg cgcattaata accacaacac agcagtgaac aatgcgccaa
     3781 tcaaaatatg ctttaaatat aaccgcacag gaaaaaatgc aaaataaaag gcggcaatga
     3841 gtaaggtggt aagcaataat gaggcgattg tggtcacaaa ttcatggcga aatagttgat
     3901 tattggccgc taaaaacgca atcacttttt cgagcatgag gccaacaaag gtaaagagaa
     3961 aaaataacag caacatgcca acaacagcaa ttaagtcttt taatttgcga cgaataaatg
     4021 aaggtgactc taccatcgtg gcaatggtgt aaaaagttga gcgcatggca ctggcgagcg
     4081 gcgtgacggc ccacaataaa acaaacaggc cggctgctcc ccaaacggcc ttatgttgtg
     4141 cagcattgta gacttccacc ataatggtac tgctaaaatt gggggctaaa gtccccatga
     4201 tgatggcaag cttcaccacg gcataatctg aactgaatac taaatgactg atggtgaaaa
     4261 acatcagtag caccatcgga ataagtgcca gcatcgcgta aaaagacaga gaggccgata
     4321 agccaaacgt attatgccgc cgaaatgcgt ttaacatttc atggataaca aataaaggtg
     4381 tgctgtaacg ctgcctggca tacgcgttca gcggtttgcg aaacgcttgt tgcaggctgg
     4441 cgctaaattt aggtttattg ttgggttctt gaatcgtcaa ttctgccttc tgtaaatctc
     4501 aaagtgcttt aaacttttct gtagcggcta ccaaggctga agtcaggctg gcatccatcg
     4561 cagagtgccc tgcatcttct atcagttgaa attccgcatg tggcatggca cgagagagct
     4621 cccaagccgt tatcggcggg cataccatat cataccgtcc ttgaagaatt tgtgtgggaa
     4681 tgtgcgcaag cttttgtttg gcctcggcca gtaaatcgcg gtcacccaca aagcaatgat
     4741 gctgaatata atgaatttga attcttgcgc gtgccaattc aatttcgccg tcaatggcat
     4801 cattgtttgt tgtggctgcc cttggtaaca gcgtcataat atttccttca aacgcattcc
     4861 agcgaatcgc agcggggata cttatattaa tgtcatctga aaatatcaac tgctcatagg
     4921 ccatgattgg attatgccgt ttattttct
//
LOCUS       DAOQSI010000149         4899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_18097_length_4899_cov_4.624484, whole genome shotgun sequence.
ACCESSION   DAOQSI010000149 DAOQSI010000000
VERSION     DAOQSI010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18097_length_4899_cov_4.624484"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="PKL42_08695"
     CDS             <1..141
                     /locus_tag="PKL42_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FlhC family transcriptional regulator"
                     /protein_id="HNU67191.1"
                     /translation="CCGGEFVINKYDLNQNYVCNLCHVPSRAGKTKKAKELTYKLVSI
                     AA"
     gene            153..998
                     /locus_tag="PKL42_08700"
     CDS             153..998
                     /locus_tag="PKL42_08700"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNU67192.1"
                     /translation="MPNHWMPKTQGQPLVWPSNDVAQQPRIKPATLALLLQLASVAGV
                     FGIIWVSHFVAQQFFHAVLTFSMWSLCFLQAALSASLAVVFKQAPWWRWIHFSFPFAV
                     FVMLQWQIPNEFYLISFIVTASLFWTTFRTQVPFYPSRPAVWYEVVKLIPIQPSTKLI
                     DIGSGLGDMSMVVAKSRPNCQAEGIEIAPLPWLVSQVRARLKRSTARFQLGNYNELNF
                     ADYDIVFAYLSPAVMQALWQKAHLEMRPNSLLISLEFEIKDWPPSFIITPKDGSPKLY
                     VWRMP"
     gene            1322..2182
                     /gene="motA"
                     /locus_tag="PKL42_08705"
     CDS             1322..2182
                     /gene="motA"
                     /locus_tag="PKL42_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HNU67193.1"
                     /translation="MFIIIGYIVICGAVFGGYAMAGGHLGGLWQPLEVVMIFGGALGA
                     FVVGNDSKALKATVKVLPSLFKGSKYNKALYMDLLALLFEILTKIRKDGMMSIENDIE
                     NPEASPLFSKYPKITADHHVTDFITDYLRLMVSGNMDAFQVENLMDNEIETHHMEGHV
                     PAHCIAKLGDGLPAFGIVAAVMGVVHTMESLHLPPNELGVLIGHALVGTFLGILLAYG
                     FVGPLSGLAEQKLEEGTKMFQTVKVTLLASLNGYAPAIAVEFGRKVLYSTERPGFAEL
                     EEHVKQAKNK"
     gene            2220..3161
                     /gene="motB"
                     /locus_tag="PKL42_08710"
     CDS             2220..3161
                     /gene="motB"
                     /locus_tag="PKL42_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HNU67194.1"
                     /translation="MAEELKKTIIVKKIKKGGGGHHGGAWKIAYADFVTAMMAFFLLM
                     WLLGSTSKAQKAGISDYFKTPLMVAIKNTGGPAVGASDSIMKDTGGKDITKKQGQVKP
                     DDGSKGKEKSVDIKEAEEAMKKAETAKLEELKEKMEKAISDSPELSKFKNQLLMDLTS
                     EGLRVQIVDEQNRPMFNSSKADMQPYAKKILQEIGKVLNGVNNKVSLSGHTDAVPYAG
                     GEAGYSNWELSSDRANASRREMISGGMDESKIVRVVGLGSASLFDKDNPFNPTNRRIS
                     IIVMNKQAEENALKDSAEVNLPSNAQAPEIDEAVAKE"
     gene            3199..3747
                     /locus_tag="PKL42_08715"
     CDS             3199..3747
                     /locus_tag="PKL42_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HNU67195.1"
                     /translation="MEMKTLPITELRSLLAAVSDHGKQHLVEVEADLLQTTFLLSEAI
                     EKLGASFMAVHEAVTEQQQVINSLMEQHQFDPIETKKLEAFKQKIGNEVNAAVTGLQF
                     QDLTSQLITRTIKRVNGLKDLLHELETHGNEIDPSHEHAAIAKFLEEISHSLHAGSHA
                     LSGGLRRSVDQHDMVTGDIELF"
     gene            3888..4253
                     /locus_tag="PKL42_08720"
     CDS             3888..4253
                     /locus_tag="PKL42_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNU67196.1"
                     /translation="MAKTILAVDDSGSLRQMVAFSLKAAGYDVVQAVDGQDGLDKAKE
                     KTVDLVLTDQNMPIMDGLTLIKNLRGLGSYQKVPILMLTTESSDEMKAKGKAAGANGW
                     LVKPFDPKRLIEVVQKVIG"
     gene            4314..>4899
                     /locus_tag="PKL42_08725"
     CDS             4314..>4899
                     /locus_tag="PKL42_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNU67197.1"
                     /translation="MSIDMSQFYEVFFDEAEELLAEAERLLLGIDMENPDAEDLNAIF
                     RAAHSIKGGAATFGFMDMTEITHVLENLLDKIRKNEMELTAEHVDAFLAAKDVLKMEL
                     DGHRLATAVDEEQVADVKMMLKSLSEAGGLAIAPAPSQPAPASEPKTASVDVANPVPV
                     KIQSIVNAPAATATDGLTHFNIVLPEISDKDASNL"
BASE COUNT         1459 a          990 c         1161 g         1289 t
ORIGIN      
        1 tgctgtggtg gtgaatttgt cattaataag tatgacctca accaaaatta tgtgtgtaat
       61 ttatgccacg tgccttcacg cgcgggcaaa actaaaaagg ctaaagagct tacttataaa
      121 cttgtttcaa tcgcagctta ggtggtacag ggatgccaaa tcactggatg ccaaaaacac
      181 aagggcagcc gcttgtgtgg ccgtccaatg atgtcgcgca gcagccccgc atcaagcctg
      241 ccaccttagc attactgttg caactggcct cggttgccgg tgtatttggc attatttggg
      301 tgagtcattt tgttgcacag caattttttc acgcagttct gaccttttca atgtggagtc
      361 tgtgtttttt acaggccgct ttaagcgcta gtcttgcagt tgtctttaag caggcacctt
      421 ggtggcgttg gattcatttt tcctttccat ttgcggtgtt tgtgatgttg cagtggcaaa
      481 ttcccaatga attctacctt atcagtttta ttgtgaccgc cagcctgttt tggacaacct
      541 ttcgcacgca agtgccattt tatccatcgc ggccagcggt ttggtatgag gtggtcaagc
      601 ttattccaat acagccatct accaagctca ttgatattgg cagcggcttg ggcgatatgt
      661 caatggttgt ggcaaaatcg cgcccaaatt gccaggcaga aggcattgaa attgcgccat
      721 tgccttggtt agtgagtcaa gtgcgtgcgc ggctcaagcg ttcaaccgca cgttttcaac
      781 ttgggaatta caacgaactt aattttgcag attacgacat cgtgtttgct tatttgtcgc
      841 cagcggtcat gcaggcattg tggcaaaaag cgcaccttga aatgcgccca aatagcctgc
      901 tgattagtct tgaatttgaa attaaagact ggccgccaag ttttatcatc acgcctaaag
      961 atggttcacc aaaactgtat gtttggcgta tgccttaact gaaagtgcgg catcgtcacg
     1021 ctgtcatttt tagtctgatt ttaagcttca aaaaccttca gaaaccttca aatttacgcc
     1081 ttaaattccc cgagagattt ttactgccct gcaagcctta taaataaagg ctcccagagc
     1141 atcgctaaag gtttgtaact aaaagccgta aatacatgag tagtttattc cctgaattta
     1201 acccccaaaa taggccttta ttctacgaat tgaattgcac agtcacttgt tattctatta
     1261 acacgtaata aaaaatcagc aaagcttcac acactatttt taagaaattg gagtcggaat
     1321 tatgtttatc atcatcgggt acattgtgat atgcggtgcg gtgtttggcg gctacgccat
     1381 ggcaggcggc cacttggggg gcttatggca gccgcttgag gtggtgatga tttttggcgg
     1441 tgcgctcggg gcttttgtgg tgggtaacga cagcaaagcc cttaaagcga cagtcaaagt
     1501 actcccaagc ttgtttaaag gctcaaaata caataaagcc ttgtatatgg atttgctcgc
     1561 gctgttgttt gaaattctta ccaaaattcg taaagacggc atgatgtcga ttgaaaacga
     1621 cattgaaaac cctgaagcca gtccactttt cagtaaatat cccaaaatta ctgctgatca
     1681 ccatgtcact gattttatta ccgattattt acgcctcatg gtgtctggca atatggatgc
     1741 ctttcaagtt gaaaacctca tggataacga aatagaaacg catcatatgg aaggccatgt
     1801 gccagcgcat tgtattgcca aactgggcga tggtttgccg gcattcggta ttgtggctgc
     1861 tgttatgggc gtggtgcaca ccatggaaag tttgcattta ccacccaatg aactcggcgt
     1921 gctgattggc cacgcgctgg tgggtacgtt cttaggtatt ttgctggctt atggttttgt
     1981 gggaccactt tcgggcttgg ctgaacaaaa gctggaagaa ggcaccaaga tgttccaaac
     2041 cgtcaaagtg acgttgctgg caagcctgaa cggctatgcc cctgccattg ccgttgagtt
     2101 tggccgaaaa gtactttatt caaccgagcg cccaggtttt gccgagcttg aagaacatgt
     2161 caaacaagct aaaaacaaat aattacggct aaatatcagg actgaatgcg gagcgactca
     2221 tggctgaaga acttaaaaaa accattattg taaaaaagat taaaaaaggc ggcggcggcc
     2281 atcatggtgg tgcatggaaa atcgcctatg cagactttgt gaccgcaatg atggcgttct
     2341 ttttattgat gtggctgttg ggatcaacct ctaaagcgca aaaagctggt atttcagatt
     2401 attttaaaac gccattgatg gtcgctatca aaaataccgg tggcccagcg gttggtgcga
     2461 gtgattccat catgaaagat accggcggaa aagacatcac caaaaaacaa ggacaagtaa
     2521 aacccgacga tggctctaaa ggaaaagaaa aatcggtcga tattaaagaa gcagaagaag
     2581 caatgaaaaa agccgaaact gccaagcttg aagagcttaa agaaaaaatg gaaaaagcca
     2641 tttctgacag ccctgaacta agcaagttta aaaatcaatt attgatggat ttaacttctg
     2701 aagggctacg tgtgcagatt gtggatgagc aaaaccgccc gatgtttaac tcatccaagg
     2761 ccgacatgca gccatacgcc aaaaaaatct tgcaagagat tggcaaggtg ttaaacgggg
     2821 tgaacaacaa agtcagtttg tctggtcata ccgatgccgt accgtatgca ggcggtgaag
     2881 caggctacag caactgggaa ttatccagtg atcgcgccaa cgcctcgcgg cgcgagatga
     2941 tttcgggtgg catggatgag agcaagattg tacgtgtggt gggtttaggg tcggcctctt
     3001 tgtttgataa agacaacccg tttaacccca ccaatcgtcg tattagcatc attgtgatga
     3061 ataagcaggc tgaagaaaat gcgttaaaag atagtgctga agtaaacctg ccatctaacg
     3121 cgcaagcgcc agaaatagat gaagcagtag caaaagagta acgactaaac aaccaagcac
     3181 cttttggaat atgaatgtat ggaaatgaaa acattaccaa tcactgaatt aagaagctta
     3241 cttgctgcag tctcagatca cggcaagcaa catctggttg aagtagaagc tgatttattg
     3301 caaaccactt ttttgctcag tgaagccatt gaaaagttag gggctagctt tatggccgtg
     3361 cacgaggctg ttaccgagca gcagcaggtt attaattctc tgatggaaca gcatcagttt
     3421 gacccaattg agaccaaaaa acttgaggct tttaaacaaa aaataggcaa tgaagttaat
     3481 gcggcggtca ccggtttgca gtttcaagat ttaaccagcc agttaattac gcgcacgatt
     3541 aaacgggtga acggattaaa agatttattg catgagctgg aaacacacgg caatgaaatt
     3601 gaccccagcc atgaacatgc agctattgca aaatttttag aagaaattag ccatagctta
     3661 catgcgggca gccatgcact ttcaggtgga ttaaggcgct cggtagatca gcacgacatg
     3721 gtaaccggtg atatcgagct tttttaataa acgatttgta ggtaaatggc taaacatttt
     3781 agattttatg ccgataagta atacggatag cgataaattt aacttaataa aatacattaa
     3841 ttaagtaact taactaacta attaacatga caaagtgagt atattaaatg gcgaaaacaa
     3901 ttttagcagt ggatgactca ggttcactta gacaaatggt ggcatttagt ctcaaagccg
     3961 ctggttacga tgtggtgcaa gcggtggacg gtcaggatgg cttagataaa gccaaagaaa
     4021 aaaccgtaga cctggtacta acagaccaaa acatgccgat catggacggg ctaacactga
     4081 ttaaaaattt gcgtggctta ggctcgtatc aaaaagtgcc aattttaatg ctgaccactg
     4141 aatcaagcga cgagatgaag gcaaaaggta aggcagcagg cgctaacggt tggttagtga
     4201 agccgtttga cccaaaacgc ctgattgagg ttgtacagaa ggtgattggt taaacattac
     4261 aaggtattaa gcacgggtct agacgtgcta acaagcatat atggagtaca acaatgtcaa
     4321 ttgatatgag tcagttttac gaggtcttct ttgatgaggc agaagagctg ttagctgaag
     4381 ctgagcgttt attgctaggc atcgatatgg aaaacccaga tgcagaagat ttaaatgcaa
     4441 tctttcgagc agcccattct attaaaggtg gtgcggccac attcggcttt atggacatga
     4501 ctgaaatcac gcatgtgctt gaaaatctgc tcgataaaat ccgcaaaaat gaaatggaat
     4561 tgacggcaga gcatgttgat gcattcttag ctgctaaaga tgtattaaaa atggagttgg
     4621 atggacatcg tcttgctacc gcggtggatg aagagcaagt ggctgatgta aaaatgatgc
     4681 tgaaatctct ttcagaggcg ggcggtttag ctattgcgcc agcgccaagc cagcctgcac
     4741 cagcaagcga accaaaaacg gcttcagtag atgttgccaa tccagtgcct gtaaaaattc
     4801 aatcaattgt aaacgctccc gcagcaactg ctacggatgg cttaacacac tttaatatcg
     4861 tacttcctga aatatcagac aaagacgcaa gcaacttgc
//
LOCUS       DAOQSI010000150         4726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_19068_length_4726_cov_5.841362, whole genome shotgun sequence.
ACCESSION   DAOQSI010000150 DAOQSI010000000
VERSION     DAOQSI010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19068_length_4726_cov_5.841362"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1853
                     /locus_tag="PKL42_08730"
     CDS             <1..1853
                     /locus_tag="PKL42_08730"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNU67198.1"
                     /translation="EILDLPGNIALIRMYGGVYLLMRRYQQRWQTVNQIGEVVADVPL
                     SASDAHAEAVVFRLSSQANSEGLDSLKALWPALRGAWAEVGIASFLINTGLLLLPLFS
                     MLVYDKVVSNGVFETLWALTIGMLVYLVTDTAMRMVRAWSTERVAEDLTRKSDESLWK
                     KLVAQKDIAGGFARFLANYRDLSVSRDFVSSTYLLTLADLPFLLLYLLVIGIIAWPLL
                     IVASILVIFYAMIGFAMQLRTNRLAKESEQHNTRKLTYMGEVLGALDVVRTVPRAGTF
                     LRRWLEFSDTASDIDSARRLATSHTSTLAAGMLTLSTVAMLAAGAYLIEARALSVGGL
                     IACNLLAARAMSLVTSLFMVIGKWQDFKRAANRMESMLDTDKVREFVPRNEVQGNLAL
                     IGLTKAYPERPVALDNVTLNIAAGERIALLGRPGAGKTTLLRSLAGLSKLDAGQILVD
                     GLSLDDIASFDRAHWLAWKSQDPALFAGTLEDNLTISGSGVNSERFIRALWASGLEDE
                     LKSGRLSLGMQIDERGSNLSGGQRQKVALARTLAQPCRILLLDEPTLGLDPDSERLLA
                     DRLPHLLGAQDILIMTTHSAIMLGVAQRVIAMDAGKVVADGPREKLLRVG"
     gene            2060..2335
                     /locus_tag="PKL42_08735"
     CDS             2060..2335
                     /locus_tag="PKL42_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNU67199.1"
                     /translation="MTQIIIDHNPSQEKLKELGVSSWSIWDCEPSKFPLDFGMTESAY
                     VLEGEIRVTPQGGETVVIKAGDFVVFPKGLKSNWEVTKQLKKHYKHS"
     gene            2558..2830
                     /locus_tag="PKL42_08740"
     CDS             2558..2830
                     /locus_tag="PKL42_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNU67200.1"
                     /translation="MAKIMIEKPSADKLAALGVTRWPTWTKEVSEFPWSYSSQEVAYI
                     LEGEVTVIERDGEAVSFGAGDLVTFPSGLTCTWQVKKALRKHYQFS"
     gene            complement(2866..>4726)
                     /gene="glnD"
                     /locus_tag="PKL42_08745"
     CDS             complement(2866..>4726)
                     /gene="glnD"
                     /locus_tag="PKL42_08745"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HNU67201.1"
                     /translation="HQLAIQTLRIRLHFLSNRREDRLLFDFQNALASELGYESTARKR
                     ASEQLMQSYYRSAKFISLINEILLKSLQQLTSKAPVVTQPINAYFEARNHLLEATHAS
                     LLQQRPAAIFEAFLLLQQHPELNGMGATLLRNLQRVKKSVNRDFRQNPTHKKLFITIL
                     SQPNGVNHALRAMNLYGILGAYIPAFGKIIGQMQHDLFHVYTVDEHILNVLANLRRFA
                     KPELKHEFPLCSKLFATFDAPHILYIAALFHDIAKGRGGDHSILGKADAARFCKTHGL
                     TKADCTLVSWLVEAHLKMSSTAQKSDLSDPTVIANFALFVKDERRLTALYLLTVADIR
                     GTSPLVWNAWKARLLENLYQATKSALNQQGLYAEQAIVQRKLEAAKKLNLFGLSPSAY
                     EPLWAQFGAHYFIRYESDEIAWQSRLLTPHLYSTKSIVRTRLSPTGEGFQVMIYTHDR
                     VSLFAKICNFFAQMNYNIVEAKIYTTDHHYALNTFIILDRSLNTERYSGIFKFIESEL
                     AKKIDETTVLEAPLRGRISRQVKHMPIEAQFTLTEEIGSNNHQLEVIANDRPGLLATI
                     AQLFLSQEIELHNAKINTLGNRAEDTFLISAKDGQKLDDDHLQKLKAALASQI"
BASE COUNT         1248 a          938 c         1161 g         1379 t
ORIGIN      
        1 gtgaaatact cgatttgccc ggaaatattg cgctgattcg catgtatggt ggtgtctatt
       61 tactcatgcg ccgttatcag caacgctggc aaacggtgaa ccagattggt gaagtcgtcg
      121 cggatgtgcc attaagtgca agcgatgcgc atgccgaagc agtagttttt agattatctt
      181 cacaagccaa ttcagaagga ttggactcac tcaaagcttt gtggccagca ctcaggggcg
      241 cttgggctga ggtggggatt gccagctttt tgattaacac cggcttgctg ttgctgcctt
      301 tattttcaat gctggtttac gataaagttg tgagtaatgg cgtatttgaa acgctttggg
      361 cgctaacgat tggcatgttg gtttacttgg ttaccgacac agccatgcgc atggtgcggg
      421 cttggtcaac ggagcgtgtg gcagaagatt taacgcgtaa aagtgatgaa tcgctctgga
      481 aaaaactggt tgcgcaaaaa gatattgcag gtgggtttgc acggttcttg gccaactatc
      541 gcgacctctc tgtttcacgc gattttgttt catccactta tttacttacc ctagccgatt
      601 tacctttttt actgctttat cttttagtga ttggcattat cgcttggcca ttgttaattg
      661 tggcttcaat tttggtaatt ttttacgcca tgattggctt tgccatgcag ttacggacta
      721 atcggcttgc taaggaatct gagcagcata acacgcgcaa actcacctac atgggtgagg
      781 tgttaggggc actggatgtc gtgcgcacgg taccgcgcgc gggtactttt ttacgacgct
      841 ggttagaatt ttcagatact gcgtcagata ttgatagcgc aagacgttta gccaccagcc
      901 acaccagtac tttagcggct ggcatgctga ctttaagcac ggtggcaatg ttggcagcag
      961 gcgcttattt aattgaggcg cgcgcgcttt ctgtgggggg gttgatagcc tgtaacttgc
     1021 ttgcagcgcg cgcaatgtct ttagttacat cgctttttat ggtgataggg aaatggcaag
     1081 actttaaacg tgctgccaat cgcatggaaa gcatgctaga cactgacaaa gtacgtgagt
     1141 ttgtgccgag aaatgaagtg cagggaaacc ttgccttgat tggtttaaca aaggcgtatc
     1201 ctgaaaggcc ggtagcgctc gacaatgtta cgctgaatat tgcggcgggc gagcgcattg
     1261 cactgctagg ccgacccggg gcaggtaaaa cgactttgtt acgcagttta gccggattaa
     1321 gtaaattgga tgcaggccaa attcttgttg atggcttgtc tttagacgat attgcttcat
     1381 ttgaccgcgc acactggctc gcttggaagt cgcaagaccc agctttattt gcaggcacgc
     1441 ttgaagataa cttaaccatt tctggcagtg gcgtgaatag cgagcggttt attcgcgcgc
     1501 tctgggcttc aggattagaa gatgagttaa aaagcggccg tttgtcattg gggatgcaaa
     1561 ttgatgaacg tggcagtaat ttatcaggcg gtcagcgcca aaaagtggcc ctggcgcgca
     1621 cgcttgcaca accttgccgc atattattgt tagatgaacc cacgctaggc ctagacccag
     1681 atagcgagcg tttgttggcc gaccgtttgc cacatttgct gggcgcacag gatattttaa
     1741 ttatgacgac ccatagcgcc attatgttag gtgttgccca gcgagtgatt gccatggacg
     1801 ctggcaaagt ggtggccgac gggccgagag aaaaattatt gcgggtcgga taagtaaata
     1861 taatcgctta acagtatttt acattctgca tcagtggact tgcctgaaag tgtatccaca
     1921 tgattttatg caacttttca aggtactttc aagataagac taccctgaca cgcgtttttt
     1981 aatacaaaga atgtgttaca attcgcccat gaaccgcaag ctaattgcgg tatttttacg
     2041 aatttttaaa ggacttcata tgacgcaaat catcatcgat cacaatccat cacaagaaaa
     2101 actaaaagaa ttaggcgttt ctagctggtc aatttgggat tgcgaaccat caaaatttcc
     2161 actagatttc ggcatgactg aaagtgctta cgtgcttgaa ggcgaaattc gcgttacacc
     2221 acaaggtggc gaaacggttg tgattaaagc aggtgatttt gtcgtgtttc caaaaggctt
     2281 gaaatcaaat tgggaagtga ctaaacaatt gaaaaaacat tacaaacatt cttaattgaa
     2341 tttttaatcg taacgtttaa aataagcctt gagaaatcaa ggctttttta ttgattattt
     2401 tcctgcttga tttcgccgag aaaaaataaa tgccctgcga gcctttattt acaaggctcg
     2461 cagggcagcc aaaaaggttt gttcgttttt aagcgttaaa attgatattt ttacagatct
     2521 tttaagtgcc taaaatgatg gttaaaggag agcgatgatg gcaaaaatta tgattgaaaa
     2581 acccagtgca gataagctcg ccgcgcttgg cgtaacgcgt tggccgactt ggaccaaaga
     2641 agtttctgag tttccgtgga gttacagttc gcaagaggtt gcctatattt tagaggggga
     2701 agtgacggtg attgaaaggg atggcgaagc ggtgagtttt ggtgcgggcg acttggtgac
     2761 ttttccatca ggacttactt gcacttggca ggtaaaaaaa gcgttacgta agcattacca
     2821 atttagttga tttaacggaa tctgaacgac acttgagatg cggcgttaaa tttggctagc
     2881 taaagcggcc tttaattttt gcaggtgatc gtcatcaagc ttttggccgt ctttagcgga
     2941 aattaaaaag gtatcttcag cacggttgcc taacgtgttg attttagcgt tgtgtaattc
     3001 aatctcttgc gataaaaaga gttgggcaat ggttgcaagc aggccagggc gatcgttggc
     3061 gataacctct aattgatggt tattgctacc aatttcttca gttaaagtaa attgtgcttc
     3121 aatcggcatg tgcttcactt gacggctgat gcgaccacgt aagggagctt caagcactgt
     3181 ggtttcgtca attttttttg cgagttcact ttcaatgaat ttaaatatac cactgtagcg
     3241 ttctgtattt aatgaccgat ctaaaattat aaaagtattc agtgcgtaat gatggtccgt
     3301 tgtgtagatt ttcgcttcaa caatgttgta gttcatttgg gcaaaaaaat tgcatatttt
     3361 ggcaaaaaga ctcaccctgt catgcgtata aatcatcacc tgaaaccctt ctcctgtggg
     3421 gcttaaccgc gtgcgaacaa ttgactttgt gctgtataaa tgcggcgtta ataaacggct
     3481 ttgccaagca atttcatctg attcgtagcg tataaaataa tgtgcgccaa attgagccca
     3541 aagcggttca taggcgctgg ggcttaagcc aaacaggttg agttttttgg cggcttcaag
     3601 tttacgttga acaatcgctt gctctgcata taatccctgt tgatttaaag cacttttcgt
     3661 tgcttggtaa agattttcca gtaatcttgc tttccaggca ttccaaacca gcggactggt
     3721 tccacgaata tcggcaacag tgagcaggta aagcgcagtg agccgacgtt catcttttac
     3781 aaatagtgca aaattggcga tgaccgttgg gtcagataag tcagattttt gagcggtgga
     3841 tgacattttc aaatgcgcct caaccagcca actgactaat gtgcaatctg ctttggttaa
     3901 tccgtgtgtt ttgcaaaatc ttgcggcatc tgccttgcca agaatagaat ggtcgccacc
     3961 tctaccttta gcaatgtcgt gaaaaagcgc cgcaatatag agaatgtgcg gcgcatcaaa
     4021 cgtggcaaac aatttgctgc aaagtggaaa ttcatgcttg agctcaggct tggcaaagcg
     4081 tcgcaaatta gccagcacat ttaatatatg ttcatccacc gtatacacat gaaacaaatc
     4141 atgctgcatt tgcccgatga ttttaccaaa agcaggaatg taagccccca aaataccata
     4201 cagattcatg gcgcgaagcg catgattgac gccattgggc tgcgacaata ttgtaatgaa
     4261 caattttttg tgtgtgggat tttgcctaaa atcgcgatta acagattttt ttacccgttg
     4321 caaattacgc aacagcgtag cgcccatgcc gtttagttcc ggatgctgct gcagtaataa
     4381 aaaagcctca aaaatggcgg cggggcgttg ttgcaataga cttgcatgcg ttgcttctag
     4441 taaatgattt ctggcttcaa aatatgcgtt gatgggttgg gtgacgactg gcgctttcga
     4501 ggttagttgt tggagtgatt ttaataaaat ttcatttatt aaactgatga attttgcgct
     4561 gcgataataa ctttgcatca gttgctcgct ggcgcgttta cgggcggtac tctcatagcc
     4621 cagctcagat gccaaggcgt tctgaaaatc aaataacaaa cggtcttcgc gtcggttact
     4681 taaaaaatgt aatcgaatac gcagggtttg tatggcaagt tgatgt
//
LOCUS       DAOQSI010000151         4720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_19099_length_4720_cov_4.502251, whole genome shotgun sequence.
ACCESSION   DAOQSI010000151 DAOQSI010000000
VERSION     DAOQSI010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19099_length_4720_cov_4.502251"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /gene="ccoN"
                     /locus_tag="PKL42_08750"
     CDS             <1..1054
                     /gene="ccoN"
                     /locus_tag="PKL42_08750"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HNU67202.1"
                     /translation="EWPIDLLIAVVWVSYAIVFFGTIAKRNIKHIYVANWFLGAFILT
                     VALLHIVNSAAMPASFWKSYSAYAGVQDAMVQWWYGHNAVGFFLTAGFLGMMYYFVPK
                     QAERPVYSYSLSIIHFWALIFTYMWAGPHHLHYTALPDWTQSIGMVFSLVLLAPSWGG
                     MINGIMTMSGAWHKLRTDPILRFLIVSLSFYGMSTFEGPMMAIKTVNSLSHYTDWTVG
                     HVHSGALGWVGMVSMGSLYFMVPRLFGQKQMHSVKAIELHFWLATIGIVLYISALWIS
                     GVMEGLMWRAINADGTLTYTFIESVKAKTPYYIIRMLGGLLYLSGMTIMLWNVIKTAT
                     SGKAVTVAVPPVHAHA"
     gene            1066..1692
                     /gene="ccoO"
                     /locus_tag="PKL42_08755"
     CDS             1066..1692
                     /gene="ccoO"
                     /locus_tag="PKL42_08755"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HNU67203.1"
                     /translation="MSNHKEKIGNNHEKVETNNFLMIVLILLTVSLGGLVEIVPLFFQ
                     KSTTEPIKGLMPYTSLQLAGRDIYQKEGCHNCHSQMIRPFRAETLRYGHYSVAGESVY
                     DHPFQWGSKRTGPDLARVGGRYSDDWHRIHLIQPRDLVPDSIMPAYPWLEENLVNAEA
                     MPSHMKALRAVGVPYTDADIAGAVDEVKGKTEMDALIAYLQVLGIHLK"
     gene            1705..1842
                     /locus_tag="PKL42_08760"
     CDS             1705..1842
                     /locus_tag="PKL42_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HNU67204.1"
                     /translation="MDINTLRIVATILSFIVFIGIAVWVWRHRKSSDFKEAANLPFKE
                     D"
     gene            1909..2814
                     /gene="ccoP"
                     /locus_tag="PKL42_08765"
     CDS             1909..2814
                     /gene="ccoP"
                     /locus_tag="PKL42_08765"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HNU67205.1"
                     /translation="MSDFTSNFWPVFITVIAVGGIVACGLGLWLTSRIKVASKPGDNT
                     SGHIWDEDIREMNNPLPRWWVGMFILTIVFSLFYLVAYPGAGSYVGKLGWTSANQYDK
                     EVAEANKALEPLYAKFAALSPEDLSKNPEAKAIGERLFMNNCAQCHGSDAKGSRGFPN
                     LTDNDWLWGGSHEKIHETLVGGRVGMMPPMAAAVGNEDDVKNVANYVLSLSGSMHDSA
                     RAGLGKEKFATCAACHGAEGKGNPDIGAPNLTDSVWLHGAGEAAIIKRINEGKMNQMP
                     AWESKFTPAQIQVLAAYVWGLSNNK"
     gene            2899..4389
                     /gene="ccoG"
                     /locus_tag="PKL42_08770"
     CDS             2899..4389
                     /gene="ccoG"
                     /locus_tag="PKL42_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HNU67206.1"
                     /translation="MSEPSSNANSAKAKEAKIPLEDVMVSLYASQDKIYPRSVSGFFT
                     SWRWVMIWATQIFFYGVPWLNWGSRQALLFDLEAKRFYIFNLVFYPQDLIYLTAILII
                     SALSLFLFTAIAGRLWCGYACPQTVYTEIFIWMERKIEGDRAARMKLDAAPMSANKAF
                     RKTAKQVAWIVFALWTGFTFVGYFTPIRELATEVMTYSLGPWETFWILFYGFATYGNA
                     GYMREQVCKYMCPYARFQSAMFDDDTLIVTYDEARGEPRGSRSRKAEVNQAKGTDKQL
                     GACIDCTLCVQVCPTGIDIRDGLQYECIGCGACADVCDTVMDKMGYERGLVKYSTQNA
                     VTKHWSHQQMIQRIFRPRVLIYSAVLLALIVAVVASLWLRTPFRVNVMRDGVMARLSD
                     DGKIENVYRLQIMNGTETTQHYVLNVTGLEDVEIETEATKDVEGDDEHHLKSIEVKPA
                     ESRSVIVDLKIPDGTLDSGSHKIKFEIQSLEGQQTVTEKSVFLVPR"
     gene            4443..4715
                     /locus_tag="PKL42_08775"
     CDS             4443..4715
                     /locus_tag="PKL42_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HNU67207.1"
                     /translation="MEKLDTKPWWRYGYVWFLMAGPFIVIIAGFITAYLAISRPDPVI
                     DDNYYRNGIEINKTLNEKRDSLAPAGQARNHAATGIKPTENAPVQP"
BASE COUNT         1271 a          909 c         1177 g         1363 t
ORIGIN      
        1 agagtggcca attgatttgc tgattgcagt ggtatgggtg tcttatgcaa tcgtcttctt
       61 tggtactatc gccaaacgta acattaaaca tatttacgtt gcaaactggt ttttaggggc
      121 gtttattctg actgttgctt tattgcatat cgttaacagt gcggcgatgc cagcgagctt
      181 ctggaaatca tactcggctt atgcgggtgt acaagatgcg atggtgcaat ggtggtacgg
      241 ccataatgca gttggtttct ttttaactgc gggcttcttg ggcatgatgt actactttgt
      301 gccaaaacaa gcagaacgtc ctgtgtactc ttacagcttg tctattattc acttttgggc
      361 attgattttt acgtacatgt gggcgggtcc tcaccatctt cattacacag cgttgcctga
      421 ctggacacag tcgataggca tggtgttttc actcgtgtta ttggcaccaa gctggggcgg
      481 tatgatcaac ggtatcatga cgatgtctgg cgcgtggcat aaattgcgta ctgacccaat
      541 cttacgtttt ttaattgttt cactctcttt ctacggcatg agtacttttg aaggtccgat
      601 gatggccatt aaaacggtga actcactctc tcactataca gactggacag tcggtcacgt
      661 gcattctggt gctttgggtt gggtgggcat ggtatcaatg ggttctctgt actttatggt
      721 gccaagattg tttggtcaaa aacaaatgca cagtgtgaag gcgattgagt tgcatttttg
      781 gttggcgacc atcggtattg tgctctatat ttcagcgctt tggatttcag gtgtgatgga
      841 aggtttaatg tggcgtgcaa ttaatgcaga tggcacatta acttacacat ttattgaatc
      901 tgtaaaagcg aaaacccctt actacattat tcgtatgttg ggtggcttgc tctacctgag
      961 tggtatgacg attatgttgt ggaatgtgat taaaacagca acaagcggta aagctgtgac
     1021 agttgcggtt cctccagttc atgcacacgc ttaaggaaaa caattatgtc aaatcataaa
     1081 gaaaaaatcg gtaataacca cgaaaaagta gaaaccaata actttttgat gatcgttttg
     1141 attttgctca cagtttctct tggtggattg gtggaaattg tgccactgtt cttccaaaaa
     1201 tcaacgactg agccaattaa ggggttgatg ccttatacgt cgttacaatt agcagggcgt
     1261 gatatatacc aaaaagaggg ttgtcataac tgccattcac aaatgattcg tccattccgt
     1321 gcggaaacat tacgctatgg tcattactct gttgcgggtg agtccgttta cgatcatcca
     1381 ttccaatggg gtagtaaacg gacaggtccg gatttggccc gcgttggcgg tcgttacagc
     1441 gatgactggc atcgtattca cttgattcaa ccacgcgatt tggtgcctga ttccatcatg
     1501 cctgcatatc cgtggttaga agaaaatcta gtgaacgcag aagccatgcc ttcacacatg
     1561 aaagcgcttc gtgcagtggg tgtgccatat accgatgctg atatcgcagg ggctgtggat
     1621 gaggtaaaag gcaaaaccga gatggatgcg ctgattgcct acctgcaagt gcttggtatt
     1681 cacttaaagt agggagcagt caacatggac atcaatacac ttcgcatagt agcaacgatt
     1741 ttaagcttca tcgtgttcat aggtattgct gtatgggttt ggcgccatcg aaagtctagc
     1801 gactttaaag aagcggccaa cctgcctttt aaagaagatt aaggtgaata aacgctagta
     1861 aataagcaag catgcttacg actatagtaa aagctaaagg aaaataaaat gagcgatttt
     1921 acaagtaatt tttggccagt gtttatcacg gtcatcgccg ttggcggcat tgttgcctgc
     1981 ggtctcgggc tctggctaac cagcagaatt aaagtggcga gtaagccggg ggataatacc
     2041 agtggccaca tttgggatga agatattcgt gaaatgaata acccgttacc acgctggtgg
     2101 gtgggtatgt ttattttgac gattgttttt tctctgttct atttggttgc atacccaggt
     2161 gcaggcagtt atgtaggtaa attaggctgg acttctgcaa atcagtatga caaagaagtg
     2221 gctgaagcca ataaagcctt ggagccttta tatgctaaat ttgcggcgtt aagtcctgaa
     2281 gatttgtcaa aaaaccctga agcaaaagcg attggcgagc gtctgttcat gaataactgt
     2341 gctcaatgcc acgggtcaga tgcaaaaggt agccgcggtt ttccaaactt gacggataat
     2401 gattggctgt ggggtggtag tcatgagaaa attcatgaaa ctttagttgg cggtcgtgtg
     2461 ggcatgatgc cgccaatggc tgcagcggtc ggtaatgagg acgatgttaa aaacgtagcc
     2521 aattatgtac tcagcctttc tggcagtatg catgattctg cccgtgcagg tttaggcaaa
     2581 gaaaaatttg caacttgtgc cgcatgccat ggcgctgaag gtaaaggtaa tccagatatt
     2641 ggtgcgccaa atctgaccga tagcgtttgg ttgcatggtg caggagaagc tgcaattatc
     2701 aaacgtatta atgaaggtaa aatgaaccaa atgccagcgt gggaatcgaa attcacgcct
     2761 gcgcaaattc aagtgctagc agcttacgtt tggggtttgt caaacaacaa gtagttgctg
     2821 ttgttattca ggcccccatc aatggtgtta gtattgatgg ggacttttca attttgtaag
     2881 acatacaagg tatgttaaat gagcgaacct tccagtaatg ctaattcggc aaaagcaaaa
     2941 gaagccaaaa tccccctaga agatgtcatg gtatcgctct atgcatcgca ggataaaatt
     3001 tatccgcgca gtgtgtctgg cttttttact agttggcgtt gggtcatgat atgggccacg
     3061 cagatttttt tctatggtgt gccttggctt aactggggta gccgccaagc gctacttttt
     3121 gatttagagg ctaaacgttt ttatattttt aatttagtct tttatccgca agacttaatt
     3181 taccttaccg ctatcctcat tatttctgct ttatcgctgt ttctctttac cgctattgca
     3241 ggccgtttat ggtgtggcta cgcctgcccg caaacggtat ataccgaaat atttatatgg
     3301 atggaacgta aaatagaggg ggaccgcgca gcgcggatga agttagatgc tgcgccgatg
     3361 tctgccaata aggcttttag aaaaacagca aaacaagttg cttggattgt attcgcactt
     3421 tggacaggtt ttacctttgt gggttatttc accccgattc gtgaactcgc aaccgaagtg
     3481 atgacctaca gtttaggtcc ttgggagact ttttggatat tgttttacgg ctttgccact
     3541 tatggcaacg cgggctacat gcgtgagcaa gtatgtaaat atatgtgccc gtacgcacgt
     3601 tttcaaagtg cgatgtttga tgatgatact ttgattgtca cgtacgatga agcgcgtggc
     3661 gagccgcgcg gaagccgttc tcgcaaagcg gaggttaatc aggctaaagg cacggataag
     3721 cagttaggcg cttgtattga ctgcactttg tgtgtgcaag tgtgcccaac aggtattgat
     3781 atacgtgacg gattgcaata cgagtgcatt ggctgtggtg cttgtgctga tgtgtgcgat
     3841 accgtgatgg ataaaatggg ttacgaacgc gggttagtga aatactctac acaaaacgcg
     3901 gtaaccaaac actggtcgca tcagcaaatg atacaacgca tcttccgtcc gcgcgtactc
     3961 atttattcag cagtgttgct tgcgctcatt gtggccgtgg ttgcaagttt atggctacga
     4021 acacctttta gagtgaatgt aatgcgtgat ggcgtgatgg caagattaag cgatgatgga
     4081 aaaattgaaa atgtgtaccg cctgcaaatt atgaacggca ctgaaaccac gcagcactac
     4141 gtgttaaatg tgacgggcct tgaagatgta gaaattgaga cagaagcgac aaaagatgtt
     4201 gaaggcgatg atgaacatca tcttaaatca attgaggtga agcctgcaga atcacgctca
     4261 gtgattgttg atttaaaaat acctgatgga accctggact ctggctcaca caagattaag
     4321 tttgaaattc agtcactaga aggtcaacaa acagttactg aaaaatcagt ctttttagtg
     4381 ccacgataat ttaaccagtt gtttgtaagc aactttgcac tacaaattaa aaaggaaata
     4441 gcatggaaaa actagatacc aaaccttggt ggcgttacgg ttatgtttgg tttttaatgg
     4501 cggggccatt tattgtgatt attgcaggct ttattaccgc gtatctggcc attagtcgac
     4561 ctgatcctgt cattgatgat aattactacc gtaacggtat tgagatcaac aaaacgctga
     4621 atgaaaagcg cgacagttta gcgccagcgg gacaggctag aaaccatgcg gcaacaggaa
     4681 taaaaccaac tgaaaacgca cctgtgcaac cctagaaaat
//
LOCUS       DAOQSI010000152         4717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_19121_length_4717_cov_4.811240, whole genome shotgun sequence.
ACCESSION   DAOQSI010000152 DAOQSI010000000
VERSION     DAOQSI010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19121_length_4717_cov_4.811240"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="PKL42_08780"
     CDS             <1..1091
                     /locus_tag="PKL42_08780"
                     /inference="COORDINATES: protein motif:HMM:NF021246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="poly(R)-hydroxyalkanoic acid synthase subunit
                     PhaE"
                     /protein_id="HNU67208.1"
                     /translation="GQNLRGTTVENPSDFFDSWMKTQQQAFSALRDQAVQMQSYFQNT
                     STSTDNPFTNWTKTAFQAFPMGADANFAKDTFSKTLYGNDAMQKLYELWQPMLNAIRN
                     KTLDPNNYADLADPDKIKQLFEKLFSFDLDAMSQLQKQTAHYADVYQQFGKPWSDAWT
                     NAAKSQSSNFMQGNFTQNDFQPEALILQMQAAYAMLENTTGKLFSTPAVGKDREKMEL
                     MSKCAKAISSFAACHIEYQQMMQMTGQEAMQTVVKTLAEKVESGEKFEKFDEFFALWI
                     DINEKTFNKQFQTKAFSQKRNAMTEAGFNARKLYNEIVENELAAFPIARRSEMDEVYK
                     LVYDLRKQVKSMELQIQELKTSASKTKE"
     gene            1096..2358
                     /gene="phaC"
                     /locus_tag="PKL42_08785"
     CDS             1096..2358
                     /gene="phaC"
                     /locus_tag="PKL42_08785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HNU67209.1"
                     /translation="MTNPFSNIDAKLTMKYLGDFNSKLMKGAELLTDIEEIDVGTAAK
                     QLVYEEDKLKLYRYKAETDKACGVPVLVVYALVNRPYMLDIQPDRSFIRNLLKLGLDV
                     YIIDWGYPTQSDRYLSMDDYINGYIDNCVNAVRKSAKNEKVSLMGVCQGGTFSAIYSA
                     LHPEKVQNLITLVAPFDFSTNDGLLFNWSKTMDVDALVDAYGVVPGNILNDGFLMLMP
                     FNLNIKKYIDMLDVMEDKEKLLNFLRMEKWIFDSPGQAGECLRQFVKDCYQGNKLVKG
                     KLKLGGKTVDLGKITMPILNIYASADHLVPPAATKPFNDLVGSEDKTLYEFQGGHIGV
                     FVGSRSQKELAPAIAKWLIQRDAPAPVKVAPVKATPANTEPAKVTEKPAAPDLATESK
                     AVKATLIPKVATKPAATKKETGNKPTKS"
     gene            complement(2474..3217)
                     /gene="phbB"
                     /locus_tag="PKL42_08790"
     CDS             complement(2474..3217)
                     /gene="phbB"
                     /locus_tag="PKL42_08790"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HNU67210.1"
                     /translation="MTKRIALVTGGTGGIGSCICRQLSADGFTVVANYIAPDEEKAQA
                     WLASERAAGRDMHIAMGDVSNYESCVAMMAKIKAEVGAVSVLVNNAGITKDGMFKKMP
                     LENWMDVLNINLNSVFNVTRQVVDDMLAAGWGRVINMSSINGQKGQFGQANYSAAKAG
                     MHGFTKALAQETAAKGITVNTISPGYIGTEMVMAIKQEVRDQIVSGIPVGRLGKPEEI
                     AKLVSYLASEDAAFITGANIAINGGQHMC"
     gene            complement(3291..4466)
                     /locus_tag="PKL42_08795"
     CDS             complement(3291..4466)
                     /locus_tag="PKL42_08795"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNU67211.1"
                     /translation="MGNVVIVAAKRTAVGKFGGAFNNVSAVDLGATVIRSLLESTGLA
                     GDQVDEVILGQVLTAGVGQNPARQSVIKAGLPKEVPAMTINMVCGSSLKAIMLGAQAI
                     KSGDAEIVVAGGQESMSTSPHVLNGSRNGLRMGDGKLIDSMIVDGLWDVYNDYHMGVT
                     AENVAKKYGISRQEQDDFACASQNKAEAAQKANKFVDEIVPFTIKNKKGDIVVDADEY
                     PRHGTTLEMLAGLRPAFDKEGTVTAGNASGINDGAAMVMLMSASKAKALGLKPLATIK
                     SYASTGIDPSIMGIGPISASKRCLEIAGWAVGDLDLLEVNEAFAAQAIAVNKDMNWDT
                     TKVNVNGGAIAIGHPIGASGARVLVSLLHEMQRRDAKKGLTSLCIGGGMGVALAVER"
BASE COUNT         1444 a         1069 c          886 g         1318 t
ORIGIN      
        1 tagggcaaaa cttaagagga acgacagtgg aaaaccctag cgattttttt gacagttgga
       61 tgaaaacaca gcagcaggct ttctcagcct tgcgtgatca ggcagtacag atgcagtcgt
      121 actttcaaaa cactagcaca tctactgaca acccttttac aaactggact aaaaccgcat
      181 ttcaagcatt tccaatgggt gccgatgcta attttgccaa agataccttc agcaaaacac
      241 tgtatggcaa tgatgccatg caaaaacttt acgaactttg gcagccaatg ttaaatgcca
      301 ttcgtaacaa aacactagac ccaaacaatt atgcagattt agcagaccct gacaaaatca
      361 aacagctttt tgaaaaattg tttagctttg atttagacgc tatgagccaa ctacaaaagc
      421 agaccgctca ttatgctgac gtttaccagc aatttggcaa gccttggagc gatgcatgga
      481 caaatgcagc aaaatcacag tctagcaatt tcatgcaggg caatttcaca caaaatgact
      541 ttcaacctga agcattaatc cttcagatgc aagcggcata cgcaatgctt gaaaacacca
      601 caggcaaact ttttagcaca cctgctgtag gtaaagaccg tgaaaaaatg gagctcatgt
      661 caaaatgtgc caaagccata tctagcttcg cagcatgcca tattgaatac caacaaatga
      721 tgcaaatgac tggtcaagaa gccatgcaaa ctgtagttaa aacgctggca gaaaaagttg
      781 aatcaggcga aaagtttgaa aaatttgatg agttttttgc cttatggatt gatattaacg
      841 aaaaaacttt taataaacaa tttcaaacca aagcattctc gcaaaaacgt aatgcaatga
      901 cagaagcagg cttcaatgcg cgcaaactgt ataacgaaat tgtagaaaat gaacttgcgg
      961 cttttcctat cgctcgccgt tctgaaatgg atgaagttta taaattagtc tacgatttgc
     1021 gcaaacaagt aaaaagcatg gaattgcaaa ttcaagaatt gaaaacttca gcatcgaaaa
     1081 ctaaggaata aagtcatgac gaatccgttc tcaaatattg atgcaaaact caccatgaaa
     1141 tatttaggtg attttaatag caagctgatg aaaggcgctg aactgctcac tgacattgaa
     1201 gaaattgatg ttggcactgc ggctaaacag ttagtctatg aagaagacaa gctaaaactc
     1261 tatcgctata aagcagaaac agataaagcg tgtggtgtgc cagtgttagt ggtttacgca
     1321 ctagtgaacc gtccgtatat gctcgatatt cagcctgacc gcagtttcat tcgtaatctg
     1381 cttaagcttg gtctagatgt ttatattatc gattggggct acccaacgca gtctgaccgc
     1441 tatttaagta tggatgacta catcaatggt tatattgata actgcgtaaa cgcagttcgc
     1501 aaaagcgcaa agaacgaaaa agtaagctta atgggcgttt gccaaggcgg tacattttcg
     1561 gctatttatt cagcattaca tccagaaaaa gtacagaact tgattacgct ggttgcaccg
     1621 tttgactttt ccactaacga tggcctacta tttaactggt caaaaaccat ggatgttgat
     1681 gcattagtag atgcttatgg cgttgtacca ggcaacatcc tgaatgatgg ttttttgatg
     1741 ctcatgcctt ttaacttgaa tatcaaaaaa tacatcgaca tgcttgatgt gatggaagac
     1801 aaagaaaagt tactcaactt cttgcgtatg gaaaaatgga tttttgacag cccaggccaa
     1861 gcaggtgaat gcttacgcca gtttgttaaa gactgctacc aaggcaacaa attagttaaa
     1921 ggtaagttaa aactgggtgg aaaaacagta gatttaggca aaattaccat gcctatattg
     1981 aatatctatg cgtctgctga ccacctagtg ccacctgccg ctactaagcc atttaatgac
     2041 ttagttggct ctgaagataa aactttgtat gaattccaag gtggtcatat tggcgttttt
     2101 gttggctcac gctcacaaaa agaattggcg ccagcaattg ctaaatggct tatacaacgt
     2161 gatgcgccag cgccagttaa agttgcgcca gttaaggcca caccagcaaa tactgaacct
     2221 gctaaagtga cggaaaaacc agctgcacct gacttagcca ctgagtccaa agcagttaag
     2281 gctactttaa tacctaaagt agctaccaaa cctgcagcaa ctaaaaaaga aactggcaac
     2341 aaaccaacta aatcttaacc cactaaatct taataataga tttagttaaa acaaaaagcc
     2401 ccgcaattca aattgcgggg ctttttgcta tgttttatag cttagtcacc tattcacttg
     2461 gtgacataat caactaacac atatgttgac cgccattaat agcaatatta gcgccagtaa
     2521 tgaacgccgc gtcttcagat gctagataag agaccaactt agcgatttct tcaggtttac
     2581 ccaaacgacc cactggaata ccagaaacaa tttgatcgcg cacttcttgt ttgattgcca
     2641 tgaccatttc agtaccgata tagcctggtg aaattgtatt gactgtaatg cctttagccg
     2701 cggtttcttg cgcaagcgct ttagtgaaac cgtgcatacc cgcttttgca gcagagtaat
     2761 tagcttggcc aaactgacct ttttggccgt taatagatga catattaatc acacgccccc
     2821 aacctgcagc caacatatca tccactactt gacgcgttac attaaacacg ctgttgaggt
     2881 taatgttaag cacatccatc caattttcga gtggcatttt tttaaacata ccatctttgg
     2941 taatgcctgc gttgtttacc aacacgctta cagcacccac ttcagctttg attttagcca
     3001 tcattgccac gcatgattca tagttagaca cgtcacccat ggcgatatgc atatctctac
     3061 cagccgcacg ctctgaagcc agccaagctt gagctttctc ctcgtctggc gcaatataat
     3121 ttgctactac tgtaaaacca tctgcagaaa gctgtctgca aatacagctc ccgataccac
     3181 ctgtgccgcc tgttaccaat gcaatacgtt tagtcatgtt tctctccttg attacgattt
     3241 tgttataaat ctttaaatat caaaaaataa cttaattttt atcctagtat ttatctttca
     3301 acggcaagtg ctacgcccat gccgccaccg atacaaagtg aagtaaggcc ttttttagca
     3361 tcacgacgtt gcatttcatg taacaatgaa acaagtacgc gtgcgccact tgccccaatt
     3421 ggatgtccaa tcgcaatagc gccgccattc acattcactt tagttgtgtc ccagttcata
     3481 tctttattca ccgcaatcgc ttgtgctgca aatgcttcgt tgacttcgag taaatctaaa
     3541 tcaccaaccg cccaaccagc aatttctaaa caacgtttgc tggctgaaat tgggccaatg
     3601 cccataatgc tagggtcaat acctgtggat gcgtaagact taatcgtggc aagtggtttt
     3661 aaaccgagcg ctttagcttt gctggccgac attaacatca ccatggcagc gccatcgtta
     3721 atgccagatg cgttacccgc cgtgactgtg ccttctttat caaatgctgg gcgtaaacct
     3781 gcaagcattt ccagtgttgt gccatgacgt ggatattcat cggcatccac cacaatgtca
     3841 ccttttttat ttttgatggt aaacggcaca atttcatcaa caaacttgtt tgctttttgt
     3901 gcggcctcag ccttgttttg tgaagcgcaa gcgaagtcat cttgctcttg gcggctaatg
     3961 ccgtatttct tggccacatt ctctgccgtc acacccatgt gataatcgtt ataaacatcc
     4021 caaaggccat ccacaatcat gctgtcaatc agtttaccgt cacccatgcg taagccattg
     4081 cgagagccat tgagcacgtg tggtgatgta ctcatacttt cttgcccacc cgcaaccaca
     4141 atttcagcat caccactttt aatcgcttgt gcgcccaaca ttattgcttt taagctagag
     4201 ccacacacca tgtttatagt catagctggt acttcttttg gtaaacccgc tttaattaca
     4261 gactggcgtg ctggattttg ccctacaccc gccgttaaca cttggcctaa aataacttcg
     4321 tcaacctgat cacccgctaa acctgtagat tcaagcaagc tacgtatgac tgttgctcct
     4381 aagtctactg cagatacatt attaaatgcg ccgccaaatt tgcctacggc agttctcttt
     4441 gctgcaacta tcactacgtt tcccatgatg ttctcctgag ttttaactgt tgttaaatta
     4501 aagtgaatta tgatactttc agaactaaca ctataacatc aaattacgct cttgcgtcat
     4561 atatcttata taagacacaa gttgtacgat tgtaagtaaa atctatgtaa ctttctcttg
     4621 aatatagttg cgtaaaatat aatatttaac gcaacaaaac gcttcgagaa aaaattaatg
     4681 ccctgcgagc cttataaaaa aaggctcgca gggcatt
//
LOCUS       DAOQSI010000153         4715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_19130_length_4715_cov_5.713090, whole genome shotgun sequence.
ACCESSION   DAOQSI010000153 DAOQSI010000000
VERSION     DAOQSI010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19130_length_4715_cov_5.713090"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1728)
                     /locus_tag="PKL42_08800"
     CDS             complement(<1..1728)
                     /locus_tag="PKL42_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HNU67212.1"
                     /translation="MKKFLKYGLYSLGGLIAVLLIIVAIVAATFNPNDYKQKVIDLVK
                     EKKERTLTLDGDIKLTFWPKIGADLGKLSISEHKSSAEFASINGAKVSLALLPLLKKQ
                     LIVDTIYIDGANANIIQYKDGTTNFDDLLSKDDEASQDIQFDIDGVNISNSAVTYSDE
                     ATGAKYSITKFNMQSGHIALAEPVDLKADFTLNANQPKLAANAQIKGNFLIDPETKHF
                     KVKGLDSRVQGDMLDGKDLDIKISGDVDAKPEQMEFLVDSLKLVASGNFDGAKQTVDL
                     SAPALTIQKQEVSGKKVTVSVSQEKAGDIFKANLVLADMKGSPKALQSSGISGDLSAV
                     QGKRTVTGAFSSPFTGNIANLIFDLPKLAGNLDIKDPSLPNGGMKGTFNLGVHAEIKN
                     EIAKSQFNLNIDGVTKLNGDVDVAGFKKSNIKFNLNADQLDLNKLLVKSNAPAKSDGK
                     PADMSALKNITLDGKLSVGSIVYDKYRVSGLNVGVKADGDKLALSGLNVKVDDSQIKG
                     SFGISHFAKPLYTFDLDIDQLNVDKYVAATPADKKASDKPLDLSALKALNADGVLRVG
                     KFKYGKNDAT"
     gene            complement(1811..2638)
                     /locus_tag="PKL42_08805"
     CDS             complement(1811..2638)
                     /locus_tag="PKL42_08805"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNU67213.1"
                     /translation="MDIILLLKAAILGIVEGATEFLPISSTGHLILAGDLLDFMDHEK
                     RGVFDIAIQLGAILAVVWEYRTRFVNTFAGIGRDPIANRLIINLTIAFLPLAILGLAF
                     GDTIKTYLFKPIPVATAFIVGAFIILWAEKRKHTITIETVDDIRPIDALKLGLAQAVA
                     LIPGMSRSGSTIIGGLFFGLSRKAAAEFSFFLAVPTLGIASIYSMYKDRALLSVDDIG
                     VWAMGFIFAFISAMIAVRALIRYVSHHDFTIFAWYRIAFGVIVLVTAYTGLVNWTVH"
     gene            complement(2833..3225)
                     /locus_tag="PKL42_08810"
     CDS             complement(2833..3225)
                     /locus_tag="PKL42_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HNU67214.1"
                     /translation="MGLFDSVAGAVLGKVLGGNQGGMAAIAMELFNQNGGIGGIMDKF
                     KAGGLGDAASSWVSLGENLPVSADQIASVFGNNAIADMAAKFGISPDVLTSQIAQHLP
                     DVINKVTPSGAVTEESGNLLTSILGMLK"
     gene            complement(3292..4494)
                     /locus_tag="PKL42_08815"
     CDS             complement(3292..4494)
                     /locus_tag="PKL42_08815"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNU67215.1"
                     /translation="MTTIKLKIKPDDIQAKATKPPVRRPSNAARDRSVPPAKLTPAGT
                     NDRDAKQMPAKASHGEPPRQRNHRYDGTLHEPAPAKSQASDGRAPTPNVAPVDARRNA
                     NTSRDKADREGTYRNPPRRGGKVRDGFVASEFDKNRTNKAAFANKAENSQSDLPRLSK
                     VMADRGLCSRREADEWIANGWVKVEGEIIETLGTRIQPDAEIIISSYAQEYQAENVTI
                     ILHKPVGYVSGQAEDGYEPAIVLVHPDNQWEDDPELHRHQPKQFHRGMLKGLAPAGRL
                     DIDSTGMLVLTQDGRIARHLIGEDSTVEKEYLVRVTGELSEADLKRLNFGLSLDGEKL
                     KPAKVSWQNEDQLRFVLREGKKRQIRRMCEMVGLHVVGLKRIRIGSVNLGKLPVGQWR
                     YLRSEERF"
BASE COUNT         1268 a         1127 c          955 g         1365 t
ORIGIN      
        1 agtggcatcg tttttgccat atttaaattt acccacacgt agcacgccat cagcattgag
       61 tgctttcagt gcacttaaat caagcggttt gtcgctggct tttttatctg ccggcgttgc
      121 cgcgacgtat ttatcaacat tcaattgatc aatatctaaa tcaaaagtgt agagtggttt
      181 ggcaaaatgg ctaatgccaa aactgccttt gatttggctg tcatctactt ttacatttaa
      241 accacttaat gccagcttgt cgccgtccgc cttaacgccc acattcaggc cagaaacacg
      301 atatttgtca taaacaatac tgccaacact taatttgcca tcgagcgtga tatttttaag
      361 cgctgacata tccgcaggtt tgccatcgct ttttgcgggt gcgtttgatt tcaccagcaa
      421 tttattcaaa tctaattggt cggcattcaa attaaattta atgttggact ttttaaagcc
      481 tgccacatca acatcaccat tcaatttagt gacgccatct atatttaagt taaattgact
      541 cttggcgatt tcatttttaa tttcagcatg cacacccaaa ttaaaggtgc ctttcatgcc
      601 accattgggt aagctcgggt ctttaatatc caagttgccc gctaatttag gcaagtcaaa
      661 aattaagttt gcaatgttgc ctgtaaatgg agatgaaaac gcgcctgtaa ctgtccgttt
      721 accctgcact gccgataaat cacctgaaat gccgctactt tgcaaggctt taggtgagcc
      781 tttcatgtcg gccaatacaa ggttggcctt gaatatatcg ccagcttttt cctgagaaac
      841 gctgaccgtt acttttttac cgctgacttc ttgtttctga atagtcaacg caggcgcact
      901 gaggtcaacg gtttgtttgg cgccatcaaa attgcctgag gccaccagtt ttaaactatc
      961 caccaaaaat tccatttgct cgggcttggc gtcaacatct cctgaaatct tgatgtccaa
     1021 atctttgcca tccaacatat cgccttgcac gcggctatct aggcccttca ctttaaagtg
     1081 cttggtttca gggtctatca agaaattacc cttgatttga gcgttggcgg ccagcttagg
     1141 ttgattcgca ttcaatgtaa aatctgcttt taaatcaact ggttcagcca atgcaatatg
     1201 accagactgc atgttaaact ttgtgatgct atatttagca cccgtcgctt catcgctata
     1261 agtcaccgca gaattgctga tattgacgcc atcaatatca aattgaatgt cttgtgatgc
     1321 ctcatcgtct ttgcttaata agtcatcaaa gttggtggtg ccatctttat attgaatgat
     1381 attggcattt gcgccatcaa tataaatggt gtcaacaatt aactgctttt tgagtaatgg
     1441 caatagcgcg agagacactt ttgcgccatt gatagaagca aactcggcgc tgcttttgtg
     1501 ttcagaaatt gatagcttgc ctaaatccgc gccgattttt ggccaaaaag tgagcttaat
     1561 gtcaccatcc agcgttaagg tgcgctcttt tttttctttg actaaatcaa tgactttttg
     1621 cttgtaatcg ttcgggttga acgttgcggc cacaatcgcc acaataataa gcaacactgc
     1681 aattaagccg cctaggctgt acaagccgta tttaagaaat tttttcatcg cccaccttca
     1741 ttcaagttta ttgctaacga cttgtccatt tctaagtctg catcgcggat accgcaattt
     1801 tgaaacgaaa ttaatgcacc gtccagttca ctaagccagt ataagccgtc accaagacaa
     1861 tgacaccaaa tgcaatgcga taccatgcaa aaatcgtaaa atcatgatga ctcacataac
     1921 ggattaacgc gcgtacggca atcatggcgc tgataaatgc aaaaataaag cccatggccc
     1981 aaacaccaat gtcgtcgacg ctgagtaatg cgcggtcttt gtacatcgaa taaatagaag
     2041 caatgcccaa cgttgggaca gccaaaaaga acgaaaattc agccgcggcc ttgcgcgata
     2101 aaccaaaaaa caagccgcca ataatggttg aaccagagcg cgacatgcca ggaatcagcg
     2161 ccaccgcttg tgctagccct aattttaagg catcaatcgg gcgaatgtca tctactgttt
     2221 ctatcgtaat tgtatgcttg cgtttttcgg cccacaaaat aatgaaagcg cccacaataa
     2281 aagcagttgc cacaggtatc ggcttaaaca agtacgtttt aattgtatcg ccaaaggcta
     2341 agcctaaaat agccaaaggt agaaaggcga ttgtcaaatt gatgatgagg cgatttgcaa
     2401 ttgggtcgcg cccgatgccg gcaaacgtgt ttacaaatcg ggtgcgatac tcccacacca
     2461 ccgctaaaat agcgcctaat tgaattgcaa tatcaaacac gccacgtttt tcgtggtcca
     2521 taaaatccag taaatcgccc gctaaaataa gatggccggt actcgatatt ggtaaaaatt
     2581 cagttgcgcc ctcaacaatg ccaagaatgg cggctttaag taataatatg atatccatag
     2641 cgttatttta acaatattta agcgttataa agcaccttat aaaagtgccg agaaattttt
     2701 tatgctctgc aagcctttat ttataaggct tgcagagcat cgaaaaaggt tgtggttaaa
     2761 aatggcataa aaaacatctt ttttgcacca aacaaaaaag gggcgcgaag ccccctcagc
     2821 atctgcctaa agctatttta acatgcccaa aatagacgtt aataagttgc cagattcttc
     2881 tgtcactgcg ccacttgggg tgactttgtt aatcacatca ggtaaatgct gcgcaatttg
     2941 gctggttaat acatctgggc taatgccgaa ttttgccgcc atatctgcga ttgcgttatt
     3001 gccaaacacg ctggcaattt ggtccgccga aacaggcaag ttttcaccca gacttaccca
     3061 tgaagatgcc gcatcgccca atccgcctgc tttaaattta tccataatgc cgccaatgcc
     3121 gccattttgg ttaaacaatt ccatagcaat ggctgccata ccgccttggt taccacctaa
     3181 cactttacct aaaaccgccc ctgcaacact atcaaataaa cccatgatga aacccctttt
     3241 taataaaata aatgcctaaa tttagcctgt cacagcactt gttagcataa ctcaaaaacg
     3301 ttcttcacta cgcagataac gccactggcc aaccggcaat ttacctaagt ttacactccc
     3361 aatgcgaata cgtttcaacc ctactacatg caggccgacc atttcgcaca ttcggcgaat
     3421 ttggcgtttt ttgccttcac gcaatacaaa gcgtaattgg tcttcgtttt gccagctgac
     3481 ttttgcaggc ttgagttttt caccatctaa acttaagcca aagttcaggc gctttaaatc
     3541 tgcttcactg agttcaccgg taacgcgcac taggtattct ttttcaactg tagagtcttc
     3601 accaattaaa tgtcgcgcaa tgcggccatc ttgagtgagc actaacatcc cagtagagtc
     3661 aatgtctaag cggcccgctg gcgccaatcc ctttagcata ccacggtgaa attgctttgg
     3721 ttgatgccga tgcaactcgg ggtcatcctc ccactgatta tccggatgca ccaatactat
     3781 cgctggctca tagccgtctt ctgcctgacc gctcacatac ccaacgggct tatgcaaaat
     3841 aatggtcaca ttttcggctt ggtattcttg ggcataacta ctgatgataa tttctgcatc
     3901 tggttgaatg cgcgtgccta gtgtttcaat gatttcgccc tctactttta cccagccgtt
     3961 ggctatccac tcatcggctt ctctgcgcga gcatagtcct cggtctgcca tcacttttga
     4021 aagtcgcggc aaatcagact gactattttc tgccttatta gcaaaagcag ctttattggt
     4081 gcgattttta tcaaattcgc ttgccacaaa accatcgcgc accttgccgc cacgacgggg
     4141 tggattacga taagtgcctt cgcggtctgc cttatctcgg ctggtatttg catttctgcg
     4201 agcgtctact ggcgccacat ttggggttgg tgcgcgacca tcagaagctt gagattttgc
     4261 tggcgcgggt tcatgtaaag tgccatcgta gcgatggttg cgttgtcgtg gcggctcacc
     4321 gtgcgatgcc tttgcaggca tttgtttggc atcacgatca tttgtacccg ccggtgtcaa
     4381 ttttgctggt ggcacactac ggtcacgcgc ggcattgctg ggacgacgca caggcggctt
     4441 ggtcgccttt gcttggatgt cgtctggttt aattttaagt ttaattgttg tcaaagccgt
     4501 aattgcccca tcagtttgaa agtcatttta ccaaaataat gcttggccct cacaattatt
     4561 actgcaaggc gccacagtct ttagtgggcc gtccatttga cttaacggtg taattggtcg
     4621 aatatattaa aacactatta agaatgacaa caacggctta tttaaacgcc gtattggata
     4681 attgaagggg atgatgatga cgttgcgaat tggtg
//
LOCUS       DAOQSI010000154         4686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_19299_length_4686_cov_3.471173, whole genome shotgun sequence.
ACCESSION   DAOQSI010000154 DAOQSI010000000
VERSION     DAOQSI010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19299_length_4686_cov_3.471173"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="PKL42_08820"
     CDS             <1..696
                     /locus_tag="PKL42_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HNU67216.1"
                     /translation="YILPDIFLTDLIQGRMGQVDGIPVMSVCETPIAGIDSIIKRLTD
                     ISLSAIVLIMISPLLLAIAFGVKLSSPGPIIFKQRRYGLDGKQINVYKFRSMTTCEDG
                     VDVVQATKEDARITPFGAFLRKTSLDELPQFFNVIQGSMSIVGPRPHAVGHNEMYRKL
                     IKGYMIRHKVKPGITGWAQVSGFRGETETLDKMQTRIEYDIDYLRNWSPRLDIYIMLK
                     TLWVIIKGQDNAY"
     gene            686..1060
                     /locus_tag="PKL42_08825"
     CDS             686..1060
                     /locus_tag="PKL42_08825"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNU67217.1"
                     /translation="MLIKEKPLRILAGCGFFIMLALLFIGGHQPASGHLFTPPWDKVA
                     HFGFFATLTLLGAIAFIRLPLALLGIMVIALGGADEIHQLFVPGRHAGLGDWAADAAG
                     CVLALIVAFWLRRQLILRFKQV"
     gene            complement(1204..2106)
                     /gene="cyoE"
                     /locus_tag="PKL42_08830"
     CDS             complement(1204..2106)
                     /gene="cyoE"
                     /locus_tag="PKL42_08830"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HNU67218.1"
                     /translation="MRTQAITLQSIGLSFKSFFSLCKPRVTSLIVFTAIIGMYMAKPA
                     TAWMPWQLLLATTLGIAFASGAAAAFNCLIEHKIDAIMARTRARALPTGQVSSVQTMT
                     FATILGGTGLAILYFYVNPLTMWLTLATFVGYAVIYTVFLKPATPMNIVIGGASGAMP
                     PILGWAAVNNTVSPESLIMFLIIFAWTPPHFWALALYRREEYAKVGMPMLPVTHGEAF
                     TLLHILLYTVILVVVSLMPYGLGMSGLIYLVATIILDAIFMAYVIGLYRKYSDDLAKR
                     TFRYSIIYLTLLFAALLVDHYWLI"
     gene            complement(2161..3246)
                     /locus_tag="PKL42_08835"
     CDS             complement(2161..3246)
                     /locus_tag="PKL42_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNU67219.1"
                     /translation="MSPVYSGFRGLANMQKSQQYFKQCVLIATILALCVVVLGAYVRL
                     TDAGLGCPDWPGCYGTLTVPESEAAIVAAQNAYPHSTVEVGKAWREMAHRYLAGTLGL
                     LVLAIFVLAWRTKKTLRCSPWTPTFLLGLVTFQALLGMWTVTMLLKPAIVTAHLLGGM
                     STLAILVWLAHRHWGQYDANMVQSRGLRLSIRFSLLLLFIQIFLGGWTSTNYAALACT
                     DFPTCHGLWVPDMDFKEAFHMVRELGQSADGGALTLAATTAIQWTHRIGALLVLIYMG
                     TLAWLLLKNSQIKRIGLALLLALIAQIGLGIANLLLHLPLVLAVLHNFTAGLLVMILV
                     TLNSKVTGNSKMPRNSKMTVASPQGFN"
     gene            complement(3209..3817)
                     /locus_tag="PKL42_08840"
     CDS             complement(3209..3817)
                     /locus_tag="PKL42_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67220.1"
                     /translation="MQTEEQFSPQQQRKGRVVLVLMLIFFVVPLLVVFSMYKYNWMPS
                     GTSVGELVKPARLMGDVSQLKNDAGSSLPNTFWKERWSIVYVTEDCQKTCLDKLHDMR
                     QLHVSLYKDMPRAQRVLITTMQDTTAIKRDYPDLIVINQPSNQISTLMAAFEVNDESV
                     ASSNRLYLVDPLGHLMMSYQPELPLAAVRKDVTRLLRFSWAG"
     gene            complement(3892..4683)
                     /locus_tag="PKL42_08845"
     CDS             complement(3892..4683)
                     /locus_tag="PKL42_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HNU67221.1"
                     /translation="MKQLQFRMHYYVFKPSWLGTVLTIICIPMFIHFGLWQYNKAQSK
                     QLIHNAFNQAQTDQALKFPTLHSNPSINVEEWKYKKVVVTGEYDTKYQFLLDNQMEGN
                     RVGYHVITPLKMDGTSKYVLVNRGWIVANDTHAEIPIFNTPSGPQTIHGQVWVPSKKI
                     FTLESKSQQAGNTLESQSWQPVWQNMNMAQYKQVVPYQISDLVIKLDQASNAGGFVRN
                     WQVPAARITTNLGYAYQWFGFALATLLIYLYMSISRVKPENQAET"
BASE COUNT         1343 a         1107 c          976 g         1260 t
ORIGIN      
        1 tatattctgc ccgacatctt tttaaccgac ctcattcaag gccgcatggg tcaggtagat
       61 ggcattcccg tgatgtccgt ttgcgaaaca ccgattgctg gcatcgacag cattattaaa
      121 cgcctcaccg atattagcct gtcggcaatc gtccttatca tgatttctcc attattattg
      181 gcgattgcct ttggcgtaaa gttaagctca cccggcccca ttatttttaa gcaacgccgc
      241 tatggcttag atggtaaaca aattaacgtc tataaatttc gctcaatgac gacatgcgaa
      301 gatggtgttg acgtggtgca ggccaccaaa gaagatgcgc gcatcacccc ttttggcgca
      361 tttttgcgca aaacttcgtt agatgaattg ccgcagtttt ttaatgtcat tcaaggttcg
      421 atgagtattg ttggcccgcg tccgcatgca gtgggacata atgaaatgta ccgcaaactc
      481 attaaaggtt acatgattcg gcacaaagta aaacctggca ttacaggttg ggcgcaggtt
      541 agcggctttc gtggtgaaac tgaaacgctg gataaaatgc aaacgcgcat tgagtacgat
      601 attgattacc tacgcaactg gagcccacgg ctcgacattt acatcatgct gaaaacgctg
      661 tgggtcatta tcaaaggtca ggataatgct tattaaagaa aagccactcc gcattttggc
      721 tggttgtggc ttttttatca tgctggcatt gctatttatc ggcggccacc agccggcttc
      781 aggccactta tttaccccgc cttgggataa agtggcgcat tttggctttt ttgctacgct
      841 caccttactt ggcgcaattg catttatacg gttgccgctg gcactactcg gcatcatggt
      901 gattgcgctg ggcggtgcag acgaaattca tcagttattt gtgcccggtc gtcatgccgg
      961 tttgggtgat tgggctgctg atgcagctgg ttgcgtgctg gccttaatag tggctttttg
     1021 gttgcgcagg caattgatat tacgttttaa acaagtgtaa attgcgccga gaaaaaaaca
     1081 atgccctgcg agcctttatt tataaggctt gcagagcatc gaaaaaggtt tgtgttaatt
     1141 taagctaaaa agtgcattta attcacgata aaaacctagc ttttctaatc ccaaatttgg
     1201 actctaaatg agccagtaat ggtccaccaa cagcgcagca aaaagcagcg ttaaataaat
     1261 aatcgaatac ctgaaagtgc gcttggccaa gtcatcagaa tattttcggt ataggccaat
     1321 cacataagcc ataaaaatgg cgtccagaat gattgttgca actaaataga ttaaaccgct
     1381 catgcccagc ccgtaaggca tgagtgaaac caccaccaaa atcacggtat agagcaaaat
     1441 atgcagcaag gtaaaagcct caccgtgcgt cactggcaac attggcatgc ccactttggc
     1501 gtattcttca cgccgataca aggccagcgc ccaaaaatgc ggtggcgtcc aggcgaatat
     1561 aattaaaaac ataatgagcg actcgggtga caccgtgtta ttgactgctg cccagcccaa
     1621 tattggtggc atcgcgcctg aagcgccgcc aatcacaata ttcattggcg tggcaggttt
     1681 tagaaacacc gtataaatca ccgcataacc cacaaaggtt gctaaggtca gccacatggt
     1741 gagagggttc acatagaaat acagtatcgc cagcccagtg cctcctaata tcgtcgcgaa
     1801 agtcatggtt tgtacagatg aaacttgccc cgttggcaaa gcccgtgcgc gcgtgcgtgc
     1861 cataatggca tcaattttat gctcaatcag gcagttaaat gcggcggccg cacctgatgc
     1921 aaatgcaatg ccgagcgttg ttgccaataa tagctgccat ggcatccagg cagtagccgg
     1981 cttagccata tacatgccaa taatggccgt aaacacaatc agcgacgtca cacgcggctt
     2041 gcacagtgaa aaaaagcttt taaagcttag ccctatgctt tgtaatgtga tggcttgggt
     2101 acgcataatc tgttctctct tattttcttg catttcaata ttagtgctgc gcatggtgac
     2161 ttaattaaaa ccttgaggag atgcaacagt catttttgaa tttctaggca tttttgaatt
     2221 tccagtcact ttagaattta aagtcactaa aatcatcacc agcaaccctg cggtaaaatt
     2281 atgcaaaaca gccagcacca atggtaaatg taataataaa ttggcaatgc ctagcccaat
     2341 ttgggcaata agcgccagta ataacgctaa gcctatgcgc ttgatctggc tattttttaa
     2401 cagcaaccaa gccaaagtcc ccatgtaaat cagcacaagc agcgcaccta ttcgatgcgt
     2461 ccattgaatg gcagtggttg cagcaagcgt taaggcaccg ccatctgcgc tttgccctaa
     2521 ctcacggacc atatgaaaag cctctttaaa atccatatct ggaacccaaa gaccatggca
     2581 ggttggaaaa tcggtacagg cgagagcagc gtaattggtg ctggtccaac cgcccaaaaa
     2641 aatctgtata aacaatagca ataaagaaaa gcgtatggat aaccttaaac cacgcgattg
     2701 caccatattg gcatcatatt gtccccagtg gcggtgagcc agccaaacta aaatcgccag
     2761 tgttgacata ccaccaagca aatgtgcagt cacgattgcc ggttttaaca acatcgtgac
     2821 tgtccacatg ccaagcagcg cctgaaatgt tacgagcccc aatagaaatg taggtgtcca
     2881 aggggaacat cgaagtgttt tttttgtgcg ccaagccaac acaaaaatcg ccaataccaa
     2941 taggcctaat gttccagcta aataacgatg cgccatttca cgccaagctt tccctacttc
     3001 aacagtgcta tgcggataag cgttttgtgc tgcaacaatg gccgcctcac tttcaggcac
     3061 agttaaggtt ccgtaacaac ccggccaatc tgggcaaccc aagcctgcat ccgtcaatcg
     3121 aacgtaagcg ccaagtacaa ccacacacag cgctaatatt gttgcgatca atacgcattg
     3181 tttaaaatac tgctgacttt tctgcatatt agccagccca cgaaaacctg agtaaacggg
     3241 tgacatcttt tctaaccgct gcgagcggta attccggctg ataactcatc atcaagtgac
     3301 caagcggatc aaccaaatac aaacggttac ttgatgcaac agactcatca ttcacctcaa
     3361 acgctgccat taaagttgaa atttgattcg atggctggtt aatcacaatt aaatcaggat
     3421 aatctcgctt aattgctgtg gtatcttgca tggtggttat taaaacacgt tgcgcacgcg
     3481 gcatgtcttt ataaagtgaa acatgcaatt ggcgcatatc atgcaatttg tctaaacagg
     3541 ttttttggca atcttcagtg acatatacaa tgctccagcg ctctttccaa aacgtgtttg
     3601 gcagactgga ccctgcatca tttttaagct gactcacatc acccattaac ctcgccggct
     3661 tcaccaactc ccccacactg gtgccgcttg gcatccagtt gtatttgtac atcgagaaga
     3721 ccacaagtaa aggaactaca aaaaatatca acatgagcac caacacaacc cggcctttgc
     3781 gttgctgttg tggagaaaat tgttcttctg tttgcatgga ttacccaaac tacttttaaa
     3841 aaaattcaag aatcattatc agcctagttt tactacggca tgataaaagt tttaagtctc
     3901 agcctgattt tctggcttta ctcggctaat actcatgtaa agatagatga gtaatgttgc
     3961 cagcgcaaac ccaaaccatt gatatgcata gcccaagttt gtggtaatac gcgctgctgg
     4021 cacctgccaa tttcgaacaa aaccacccgc attacttgcc tgatctaact taatcactaa
     4081 atcagaaatc tggtacggca caacctgttt gtattgagcc atattcatat tttgccaaac
     4141 gggctgccaa gactgtgatt ctaacgtgtt gcctgcttgt tgtgacttac tttcaagcgt
     4201 aaaaattttc ttactcggca cccacacttg tccatgaatc gtttgtggcc cagaaggcgt
     4261 attaaatata gggatttctg catgtgtatc atttgcaact atccaacctc tattcaccaa
     4321 aacatatttg ctggtgccat ccatttttaa cggcgttatc acatggtaac caacgcgatt
     4381 gccctccatt tgattatcta acaaaaactg gtactttgta tcatactcac ccgtcaccac
     4441 gaccttcttg tacttccatt cttcaacatt aatggatgga tttgaatgta acgttggaaa
     4501 cttcaatgcc tgatctgtct gtgcttggtt aaatgcattg tgaataagtt gtttactttg
     4561 cgccttgttg tattgccaca gaccaaaatg aatgaacata gggatgcaaa taatggtcag
     4621 cacagtacct aaccatgacg gcttaaaaac ataataatgc attctaaatt gcaattgttt
     4681 catatg
//
LOCUS       DAOQSI010000155         4673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_19369_length_4673_cov_5.100909, whole genome shotgun sequence.
ACCESSION   DAOQSI010000155 DAOQSI010000000
VERSION     DAOQSI010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19369_length_4673_cov_5.100909"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..1349)
                     /gene="tilS"
                     /locus_tag="PKL42_08850"
     CDS             complement(42..1349)
                     /gene="tilS"
                     /locus_tag="PKL42_08850"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNU67222.1"
                     /translation="MQANPLLLLAFSGGLDSTVLLYLLAASRQNLSFSLQAMHVHHGL
                     SSHANAWTDFCIKQCKQLDVPLHLEYINIESKSKLGVEATARKLRYQALFNFKLAELI
                     PDFIVTAHHQDDQAETLLLQLFRGAGVKGMAGMAAVDKSRRLLRPLLGTSRSALHGYA
                     IQNAITWCDDESNLNVDYERNFVRHKVVPVLHERKPSIQPVIARVATHLAEANALLDN
                     LAEIDASQVLSQNSLCLISLEKLTLARAKNLLRWWLANNQISMPNTEYLSEMLHQLLN
                     AKPDANVDMPLRHNEMLKSNQPPKSLRRYLQRAYLVSTQKSKPIDLLWHGEPCLTLPN
                     GGGLEFRHVIGAGLALKYDLSGLKITRRSGAERFKPHPLRPSRTLKHLLQEAHIPPWQ
                     RDCLPLIYWHDKLVFVPGIGAAHDMCASQDEPGVVIVWHQSQG"
     gene            complement(1474..2451)
                     /locus_tag="PKL42_08855"
     CDS             complement(1474..2451)
                     /locus_tag="PKL42_08855"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HNU67223.1"
                     /translation="MKISYLDFEQPIAELEAQIEGLQAAHDANDALDISKELTALENK
                     NKKLSEDIYGSLTAWQISQLARHPQRPYTLDYIQGIFTDFEELHGDRAYADDPAIVGG
                     LARFEGQPVMVIGHQKGRDVKERQYRNFGMPRPEGYRKAMRLFRLAEKFSIPIITLID
                     TPGAYPGIGAEERGQSEAIARNLYVMAELKTPIIGVIIGEGGSGGALALGVVDQLLML
                     QYGTYSVISPEGCASILYKSADKASVAAESLAITADRLKALGLVDRIIPEPLGGSHRD
                     PEAVMTTLRAALKEELTKLKSKSTKVLLVSRYERLMGYGKYKEIVDKKR"
     gene            complement(2497..>4673)
                     /gene="dnaE"
                     /locus_tag="PKL42_08860"
     CDS             complement(2497..>4673)
                     /gene="dnaE"
                     /locus_tag="PKL42_08860"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNU67224.1"
                     /translation="ALDGVSQIATFGTMAAKAVVRDVGRVLDLPFHFVDGISKLIPME
                     LGITLSDALVKEPQLAERRENEEELRELLELALRLEGLVRNVGMHAGGVLISPGKISD
                     FCPVYCQADGASLVSQYDKDDVEAVGLVKFDFLGLRTLTILELALKNANGQRAAEGLP
                     PLSFETLPLNDKATFQLLKTANTTAVFQLESKGMKDMLKQAMPDCFEDIIALVALYRP
                     GPMDLIPDFCRRKHGKQRVEYPHPLTEPILKETYGIAVYQEQVMQIAQVVAGYSLGGA
                     DLLRRAMGKKKVEEMDAQRKTFVEGATKNNMTERQATELFDLLEKFAGYGFNKSHAAA
                     YALVAYQTAYLKAHYPAAFLASTMSADMNNTDSVHTFYDDCAPNQIEVLPPDINQSDF
                     KFTPINKQQILYGLGAVKGTGLAAIEVILQAREQGGPFKDLFDFCNRLDLRKVNRRVV
                     ESLIRAGAFDKLSPSRNALLAGVANAMTAAEQNNANSNQNSLFGDVEDKAAHVLPSVP
                     EWSEQQRLLEEKAALGFYFSGHPYFAYQKDISQFVSATLANLSPKEQPQLIAGIVSGI
                     RIRMTGRGKMAIVGLDDGTTRIEVVVGSELLNQSQQLLKDDQLVIVEGRVSHDDFSGG
                     IRVNARKLYDLSGLRNSRASFLKIACNGQADAAKLKTMLQPYCRADTEEQRGCPVKVE
                     YHNKSSKVELMLGSAWRVDLHEELIASLTEWLSRENVKILYN"
BASE COUNT         1211 a         1168 c          979 g         1315 t
ORIGIN      
        1 tcttaattag aataacagtg gatgacaggc tacagcttca attagccttg tgattgatgc
       61 caaacaatta caactcccgg ctcgtcttgt gacgcacaca tatcatgcgc agcacctatg
      121 ccaggtacaa atacaagctt gtcgtgccaa taaatgagcg gcaaacaatc tcgttgccag
      181 ggtggaatat gcgcctcttg taacaggtgt ttgagcgttc ttgaaggtcg cagtgggtga
      241 ggtttaaaac gttcagcgcc gcttcgccgg gtaattttta atccacttaa gtcatacttt
      301 aaggctaatc ctgccccaat aacatgcctg aactctagcc ctcccccgtt cggtaaagtc
      361 aaacatggtt caccatgcca taacaaatca attggtttcg atttttgcgt ggaaaccaag
      421 taagctcttt gcaaataccg cctaagactt tttggcggtt gatttgattt tagcatttcg
      481 ttgtgacgca atggcatgtc tacatttgca tcaggttttg catttaatag ctggtgtagc
      541 atttctgata gatattctgt gttgggcatg gatatctgat tgttcgccaa ccaccaacgc
      601 aacaaatttt ttgcacgcgc taacgtcagt ttctctaacg aaatcaaaca taaactgttt
      661 tgtgataaga cttggcttgc atcaatttca gctaaattat caagcaaagc attcgcttca
      721 gccagatgtg tagccactct tgcgatgact ggttgaatag atggtttacg ctcatgcaaa
      781 accggcacca ccttatggcg cacaaaattt cgctcgtaat ctacgtttaa attactttcg
      841 tcatcacacc aagttatagc attctgtatc gcatacccgt gtaaagcgct acgtgaagtg
      901 cctagcaaag ggcgcaataa acgcctcgac ttgtcaacag cagccatgcc agccatgccc
      961 ttcacgcccg ctcctctaaa aagctgtaat aaaagcgttt ctgcctgatc atcttgatga
     1021 tgcgcagtga caataaagtc tggaatcaac tctgccagct tgaaattaaa aagcgcttga
     1081 tatcgcaact ttctagccgt tgcttcaacg cctaacttac tttttgactc gatattaatg
     1141 tattcgagat gtagcggcac atccaactgc ttgcactgtt taatgcagaa gtcagtccac
     1201 gcattggcat gcgaactcag gccgtggtgt acatgcatag cctgtagcga aaaagaaaga
     1261 ttttggcggc tggctgccag taaatataat aaaactgttg agtctaagcc accgctaaac
     1321 gctagcaata gcaacggatt tgcctgcatg ctgccgttcg ttgactgatg ctcccgttca
     1381 ccaatgttga gatgatgaac gagaaatgcg cttagttttt tttgtagggg gtgcgtgggt
     1441 tcggtggcat tagccgctgt tacacactgc gtcttagcgt tttttatcaa cgatttcttt
     1501 gtatttacca taacccatta agcgctcgta cctactgacc aacaatactt ttgttgattt
     1561 agattttaat tttgtaagtt cttctttaag tgcagcgcgc aatgttgtca tgaccgcttc
     1621 tgggtctcga tgagagccac ctaatggttc tgggataatg cggtcaacca accctaaggc
     1681 ttttaaacgg tcagcggtga ttgcgaggga ctctgccgca acggaagcct tgtccgcact
     1741 tttatataaa atagacgcac agccttcagg agaaatgacc gaataggtgc catattgcaa
     1801 catcagcagc tggtcaacca caccgagtgc cagcgcaccc ccagaaccac cctcgccaat
     1861 aatcacgcca ataataggcg ttttgagctc agccatcaca tataagttac gagcaatcgc
     1921 ctcagactgc ccacgctctt cagcaccaat cccaggatac gcaccgggcg tgtcaatcag
     1981 ggtgataatt ggaatcgaaa acttttccgc taaccgaaat aagcgcatgg ctttgcggta
     2041 accttctggt cttggcatac caaagttgcg atattgtcgc tcttttacat cccgaccttt
     2101 ttgatggcca atcaccatca cgggctgccc ttcaaaacgt gctagtccac ccacaatcgc
     2161 agggtcatca gcataagccc tatcgccgtg caattcttca aaatccgtaa atatgccttg
     2221 aatataatca agcgtataag gccgctgagg gtggcgtgcc aattgtgaaa tttgccatgc
     2281 cgttaaacta ccgtaaatat cttcagacag ttttttgttt ttattttcta gcgctgtcag
     2341 ctctttagaa atatccagtg catcattggc atcatgcgcg gcttgcaacc cttcgatttg
     2401 cgcttctagt tcggcaattg gctgttcaaa gtctaggtaa cttattttca tttgattcgc
     2461 ttcttttcta aattcaatgc gtttaattat atgcgtttaa ttatatagga tttttacatt
     2521 ttcacggctt aaccattcgg ttaagctggc aatcaattct tcatgcaagt caacgcgcca
     2581 tgcgctaccc agcatcagtt caactttact gcttttatta tgatattcca cttttacggg
     2641 gcaaccgcgc tgctcttccg tgtctgccct gcaatagggt tgcagcattg ttttaagttt
     2701 agctgcgtcg gcttgcccat tacaagcgat ttttagaaag cttgcgcgtg aattcctaag
     2761 cccagataaa tcatatagct ttcttgcatt cacacgaatg ccgccagaaa agtcatcgtg
     2821 actcacacgg ccttcaacaa tcaccagttg atcatctttt aataattgct gtgattgatt
     2881 aagcagttcg ctgccgacga ccacctcaat gcgcgttgtg ccgtcatcta agcccacgat
     2941 tgccattttg ccacggcctg tcatacgaat gcgaatacca gacacgatgc ctgcaatcaa
     3001 ctgcggttgc tctttaggtg ataaattggc gagcgtggca ctcacaaact gactaatgtc
     3061 tttttgatac gcaaagtaag gatggccgct aaagtaaaat ccaagcgcag ccttttcttc
     3121 caataagcgt tgctgctcag accactctgg cacactcggt aatacatgcg cagctttatc
     3181 ttctacatcc ccaaacaagc tgttttggtt gctgttggca ttattttgct cggcagctgt
     3241 cattgcgttt gcaacgcctg ccaacagcgc attccggcta ggggacaact tgtcaaatgc
     3301 gccggctcga attaaagatt caacaacacg acgattcacc tttcgcaaat caagccgatt
     3361 acaaaaatcg aataaatctt taaaaggccc gccctgttcg cgcgcctgca aaatcacctc
     3421 aatcgccgcc aaacccgtgc cttttacggc accaagccca tacaaaattt gctgtttgtt
     3481 gataggcgta aatttgaaat cactttgatt gatgtctggt ggaagcactt caatttgatt
     3541 cggtgcacaa tcatcgtaga acgtgtgaac actgtcggtg ttgttcatgt cagctgacat
     3601 ggttgatgct aaaaatgctg cgggataatg cgcttttaag taagccgttt gataggccac
     3661 cagcgcgtaa gctgcggcat gcgatttatt aaagccgtaa ccagcaaatt tttcaagcaa
     3721 gtcaaataac tccgtcgctt gtcgctcggt catgttgttt ttagttgcgc cttccacaaa
     3781 tgttttgcgt tgggcatcca tttcctcaac tttcttttta cccatcgcgc ggcggagtaa
     3841 atcggcacca ccaaggctgt agcccgcaac cacttgtgca atttgcatga cctgctcttg
     3901 atacacggca ataccatagg tttcttttaa aattggctca gttaaaggat gcggatattc
     3961 cacacgctgc ttaccgtgtt tgcggcggca aaaatccgga atcaaatcca ttgggccagg
     4021 ccgatacagg gccaccaaag caataatatc ttcaaaacaa tcgggcatcg cctgcttgag
     4081 catgtctttc atgcctttag attcaagttg gaatacggcc gttgtattgg ccgttttgag
     4141 taattgaaag gttgccttgt cgttcaaggg tagcgtttca aaagacagtg gtggcaagcc
     4201 ctcagccgca cgttgcccat tggcattttt gagggctaat tccaaaatcg tcagcgtacg
     4261 caagcctaaa aagtcaaact tcaccagccc gacggcttcc acatcatctt tatcgtattg
     4321 gctgaccaaa cttgcgccat cggcttgaca atacactggg cagaaatccg agatttttcc
     4381 gggtgaaatc aataccccgc ccgcatgcat gccgacatta cgtaccagtc cttctaaccg
     4441 taatgccaac tccaataatt cgcgtaactc ttcctcgttt tcacggcgct cggcaagctg
     4501 tggctctttt accagcgcat cggagagcgt aatgcccaat tccattggaa ttaacttact
     4561 gatgccatcg acaaaatgga agggcaaatc cagcacacgt ccaacatcac gcaccaccgc
     4621 tttcgctgcc atagtaccga aagtggcaat ttgcgatacg ccatctaacg cat
//
LOCUS       DAOQSI010000156         4562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_20095_length_4562_cov_4.845130, whole genome shotgun sequence.
ACCESSION   DAOQSI010000156 DAOQSI010000000
VERSION     DAOQSI010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20095_length_4562_cov_4.845130"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            302..658
                     /locus_tag="PKL42_08865"
     CDS             302..658
                     /locus_tag="PKL42_08865"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNU67225.1"
                     /translation="MLENYFPILLFIIVGLAIGVGPLLLGKLVSPNKPDDAKNSPYEC
                     GFEAFEDARMKFDVRYYLVAILFILFDLEIAFLFPWAVVLQEIGLAGFVAMMVFLGVL
                     VIGFIYEWMKGALEWD"
     gene            666..1142
                     /locus_tag="PKL42_08870"
     CDS             666..1142
                     /locus_tag="PKL42_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNU67226.1"
                     /translation="MSIEGILEKGFVTTTLDTVINYTRTGSMWPMTFGLACCAVEMMH
                     AGASRYDLDRFGIVFRPSPRQSDVMIVAGTLVNKMAPALRKVYDQMAEPRWVISMGSC
                     ANGGGYYHYSYAVVRGCDRIVPVDVYVPGCPPTAEALLYGIIQLQNKIKRTNTIAR"
     gene            1156..1752
                     /locus_tag="PKL42_08875"
     CDS             1156..1752
                     /locus_tag="PKL42_08875"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNU67227.1"
                     /translation="MTSKLQQLSENLQAQLGAKITRQTVALGEVTIECGADDYLVVCQ
                     ILRDDAHFKFEQLIDLCGLDYQAYGEGGYEGLRYAVVCHFLSVTLNQRLRLKVFAKED
                     DFPVLPTLVELWPVANWFEREAFDLFGIMFENHPDLRRILTDYGFVGHPFRKDFPMIG
                     NVEMRYDPEQKRVIYQPVSIELRNNVPRVIREEGSHHG"
     gene            1745..2998
                     /locus_tag="PKL42_08880"
     CDS             1745..2998
                     /locus_tag="PKL42_08880"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNU67228.1"
                     /translation="MAEIRNYTMNFGPQHPAAHGVLRLVLELDGEVIQRADPHIGLLH
                     RGTEKLAENRTYLQSVPYMDRLDYVSMMSNEHAYVMAIEKMLGLEVPIRAQYIRVMFD
                     EITRVLNHLLWLGAHALDVGAMTVFLYAFREREDLFDVYEAVSGARMHAAYYRPGGVY
                     RDLPNQMPQYEVSSLRNAKTVKTQNENRQGSLLDFIEDFTNRFPKYVDEYETLLTDNR
                     IWKQRTVGIGVVTPERALALGMTGPMLRGSGIAWDLRKKQPYEVYADLDFDIPVGVNG
                     DCYDRYLVRMEEFRQSNHIIKQCVAWLRKNPGPVISNDNKVAPPNREGMKTNMEDLIH
                     HFKLFTEGFHVPAGEAYAAVEHPKGEFGIYMVSDGANKPYRLKIRAPGFPHLAALDEM
                     TRGHMIADLVAIIGTQDIVFGEIDR"
     gene            3002..3493
                     /gene="nuoE"
                     /locus_tag="PKL42_08885"
     CDS             3002..3493
                     /gene="nuoE"
                     /locus_tag="PKL42_08885"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNU67229.1"
                     /translation="MLSSQAIEKIEYELTKYPADQRQAAVMSALRIVQSEKGWLSKES
                     ISDVAQYLGMPEIAAMEVATFYNMYDLTPVGDYKITVCTNISCMLRGSDEIVSHLKAK
                     LGIGFNEITSDGKFCLKEGECMGCCGGAPLMHINNTQMHEFLTPEKVDEILGGLDKKG
                     AQS"
     gene            3490..>4562
                     /gene="nuoF"
                     /locus_tag="PKL42_08890"
     CDS             3490..>4562
                     /gene="nuoF"
                     /locus_tag="PKL42_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNU67230.1"
                     /translation="MTAPNFHHLEKQTLMALRTVTEANPRSLETYVKLGGYEQLKRIV
                     TQKIKATDIIAEVKVSGLRGRGGAGFPTGLKWSFMPRYFDGTKYIVCNTDEGEPGTFK
                     DRDIIRYNPHQLIEGMAIAAYTLGARVGYNYIHGEIWQDYETFEAALAEARAAGFLGE
                     NLFGTTFDFDLYAVHGYGAYICGEETALIESIEGKKGQPRFKPPFPASYGVFGKPTNV
                     NNTESYTSIPWILQYGGKAFADLGVPNSGGTKLFSVSGHVTNPGNYEVKMGTPFTELL
                     EMAGGVWKGRKLKAVIPGGPSTAVLPAAAMMAANMDYDSLSKARSSLGAGSMIVMDET
                     TCMVKTLHRLSYFFYEESCGQCTP"
BASE COUNT         1201 a          820 c         1159 g         1382 t
ORIGIN      
        1 aaatgttgtt atgttccggt ttatgctgag aatgtgaaga agagttaata ggcatagttt
       61 tacagcctta atcattcaaa tgcatcattg cgtcatcaag cataatagct attgaaaaat
      121 aaacattagc gcgtatgttt cttttgaaag cttatgtgta ttgggttaca atcttgctat
      181 agcctgaatg ggtagatagc tgtggctcgg tagtgatagt gcgtagtgtt taaagctatt
      241 ttgagtgttc tagtttattt atgttttggg cagtttaaga atttcaattt ggggctagcc
      301 ggtgctagaa aattactttc caatattatt gtttatcatt gttggtctcg ctataggcgt
      361 tggccctctc ctattaggta agcttgtttc acccaataaa cccgacgatg caaaaaactc
      421 tccttatgag tgtggctttg aggcctttga agatgcgcgg atgaagtttg acgtgcgtta
      481 ttatcttgtg gctattctat ttattctttt tgatttagag attgcctttt tattcccttg
      541 ggctgttgtt ttgcaagaaa ttggtttggc tggttttgtt gcgatgatgg tctttttagg
      601 ggtgctggtg attggtttca tctatgaatg gatgaagggt gcattagagt gggattaatt
      661 ctggcatgag tattgagggt attttagaaa aagggtttgt taccacaaca ctggatacgg
      721 tgattaacta tacacgcacg ggttcaatgt ggcccatgac gtttggtttg gcctgctgtg
      781 cggttgagat gatgcatgcg ggtgcttcac gctacgattt agatcgtttt ggtattgtgt
      841 tccggccaag tccgcgccaa tcagatgtaa tgattgtcgc tggtactttg gttaacaaaa
      901 tggcaccagc cttacgcaag gtttatgatc agatggccga acctcgctgg gtaatttcca
      961 tgggttcgtg tgccaatggt ggtggttatt accattattc ttatgcagtc gttcgtggct
     1021 gtgaccgtat cgtgccagta gacgtgtatg ttcctggttg ccctcctaca gctgaggctt
     1081 tgctttatgg cattattcag ctccaaaata aaattaaacg cactaatacg atagcgagat
     1141 aagagcgcta acgacatgac aagtaaatta caacaattat cagaaaatct gcaggcacaa
     1201 cttggtgcga aaatcactcg gcagacagtt gcgttaggtg aggtcacaat tgagtgtggc
     1261 gcagatgatt atctagtcgt ttgtcaaata ttacgtgatg atgcgcattt taaatttgaa
     1321 caactgattg atttgtgcgg tttggattat caagcatacg gcgaaggtgg ctatgaaggc
     1381 ttgcgttatg ctgtggtatg ccacttttta tctgtaactt tgaatcagcg cttacgattg
     1441 aaggtttttg ccaaagaaga tgactttccc gtgttgccta ctttggttga gttgtggcct
     1501 gtggcgaatt ggtttgagcg tgaagcgttt gatctctttg gtattatgtt tgaaaaccat
     1561 ccggatttac gccgtatctt gacggattat ggttttgtcg gtcatccgtt ccgtaaagat
     1621 ttccccatga taggtaacgt tgaaatgcgt tatgacccag agcagaagcg cgtgatttat
     1681 cagcctgttt ctatagagtt gcgtaataat gtaccacgtg tgattcgtga ggaaggatca
     1741 catcatggct gagattagaa actatacaat gaactttggc cctcagcacc cggcggcgca
     1801 tggcgtgttg cgtttggtgc tggagttgga tggcgaagtt attcaacgtg ctgatccgca
     1861 cattggcttg ttgcatcgag gtacggaaaa gctcgcagaa aatcgcacct atttacaaag
     1921 tgtgccctat atggataggc tcgattacgt gtcgatgatg tccaacgagc acgcgtacgt
     1981 tatggctatt gagaaaatgc taggccttga agtacccatt agagcgcaat atattcgtgt
     2041 catgtttgat gagattacac gtgtgttaaa tcacttgctc tggttaggtg cgcatgcact
     2101 tgacgttggc gcgatgacgg tgtttttgta tgcatttcgc gaacgcgaag atttgtttga
     2161 tgtgtatgaa gcggtttctg gcgcgcgcat gcacgcggcc tattaccgcc caggtggtgt
     2221 ttatcgtgat ttgccaaacc aaatgccgca gtatgaagtg tcatcactac gcaacgctaa
     2281 gacggttaaa acccaaaatg aaaatcgcca aggttcttta cttgatttta ttgaggattt
     2341 taccaatcgt tttccgaagt atgttgatga atatgaaaca ttgctcacag ataatcgtat
     2401 ttggaagcaa cgcactgttg gcatcggcgt ggtgacgccg gagcgtgcgc tggcactggg
     2461 gatgactggg ccaatgttgc gaggttctgg tatagcttgg gatttacgca aaaagcagcc
     2521 ttatgaagtc tatgcagact tggattttga tattccagta ggcgtgaatg gtgactgcta
     2581 tgaccgctac ttggttcgta tggaagaatt taggcaatca aatcacatta ttaagcaatg
     2641 tgtcgcgtgg ttacgcaaaa acccaggccc agttatttca aatgataata aagtcgcacc
     2701 gcccaaccgt gagggtatga aaaccaacat ggaagactta atacatcatt ttaaactttt
     2761 tacagaaggt tttcatgtgc ctgctggtga agcctatgct gcggttgaac atcctaaagg
     2821 tgagtttggt atttatatgg tgtcagatgg tgcgaataag ccgtatcgct taaaaattcg
     2881 tgcgcctggt tttccgcact tagctgcatt ggatgaaatg actagaggtc atatgattgc
     2941 ggatttggtg gccattattg gcacgcaaga tattgtattt ggcgaaatag atagataaaa
     3001 aatgttaagt tcacaagcta ttgagaaaat tgaatacgag ctgacgaaat accctgcaga
     3061 tcaacgtcag gcagcggtta tgagtgcatt gcgtattgtg caatcagaaa aaggctggtt
     3121 gtctaaagaa tccatttcag atgttgccca atatttgggt atgccagaaa ttgcagcaat
     3181 ggaagtagca actttttaca atatgtatga tttaacgcca gttggcgatt acaaaattac
     3241 cgtatgtacc aatatttcct gcatgctgcg tggttcagat gaaatcgtga gtcatctaaa
     3301 agccaaactc ggaattgggt ttaatgaaat aacctctgac gggaagtttt gcctaaaaga
     3361 aggtgagtgc atgggctgtt gtggcggagc acctttaatg catattaaca atacacaaat
     3421 gcatgaattt ctgacgccag aaaaagtgga tgagattttg ggtggtttag ataaaaaagg
     3481 cgcgcagtca tgacagcacc caattttcat catctagaaa aacaaacgct catggccttg
     3541 cgtacagtta ccgaggcgaa cccacgcagt ctggaaacct acgtcaaatt aggtggttac
     3601 gaacaattaa agcgtattgt tacacaaaaa atcaaagcga cagatattat tgccgaagtt
     3661 aaggtgtcag gtttacgcgg gcgtggtggc gctggttttc ctacggggct caagtggagc
     3721 tttatgcctc gatattttga tggtacaaag tacatcgtat gcaataccga tgagggtgag
     3781 ccgggcacat ttaaagaccg cgatattatc cgatacaatc cgcatcaact tattgagggt
     3841 atggcgatag ccgcttatac gctgggtgcc agagtaggct acaactatat tcatggtgaa
     3901 atttggcaag attatgaaac ttttgaagcg gctttggctg aagcaagagc ggcagggttt
     3961 ttaggcgaaa atctatttgg cactaccttt gattttgatt tgtacgcagt gcatggctat
     4021 ggtgcctata tttgcggtga agaaaccgct ctaattgagt ccattgaagg taaaaaaggt
     4081 cagccacgct ttaaacctcc ttttccggcc agttatggtg tatttggtaa gccaaccaac
     4141 gtgaataata ccgaaagtta tactagtatt ccatggattt tgcagtacgg aggaaaggca
     4201 tttgcagatt taggtgtgcc aaattctggc ggcactaaat tgttttccgt atctggacat
     4261 gtgacaaacc ctgggaatta tgaagtgaaa atgggtacac cttttactga gttgcttgaa
     4321 atggcggggg gtgtctggaa agggcgcaag cttaaagcgg tcattcctgg aggcccttct
     4381 acagccgtat tgcctgcggc agctatgatg gcggcaaata tggattacga tagtctaagt
     4441 aaagcgcgtt caagcttagg tgccggctca atgattgtga tggacgaaac gacttgtatg
     4501 gtcaaaactt tgcatcgttt gagttatttc ttttatgagg aaagttgcgg acaatgtacg
     4561 cc
//
LOCUS       DAOQSI010000157         4553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_20139_length_4553_cov_3.943308, whole genome shotgun sequence.
ACCESSION   DAOQSI010000157 DAOQSI010000000
VERSION     DAOQSI010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20139_length_4553_cov_3.943308"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PKL42_08895"
     CDS             complement(<1..306)
                     /locus_tag="PKL42_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HNU67231.1"
                     /translation="MDKMTEIIVQVRDAGETINTAAGEISSGNTDLSSRTEQQASSLE
                     ETASSMEELASTVKQNAENAKQANQLAAAASGVAIKGGEVVGQVVATMSSINESAKKI
                     "
     gene            complement(436..1974)
                     /locus_tag="PKL42_08900"
     CDS             complement(436..1974)
                     /locus_tag="PKL42_08900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNU67232.1"
                     /translation="MFASIINKLASGTQATKKIIDEVTEFRALKAEINKARCVLEFDA
                     KGTIIAINQNALKALGFSESDVLNEHHRVLVGSTESARPEYEDFWENLHGGTTQTGKF
                     KFKNKKGEDVWFQGYYAPVINSKHQLIKVVSYLTDITAELTRALVLQGEDEALNQTFG
                     VMECDMAGNITECNDLFMQPLGYRREELVGHHVGVLLPPEVAQSPEYKKMWEELNAGI
                     SQTRQIRRLSKDRKEYWFQSTYVPIKDEAGKSYKVVVYSYCITAEKIKNADFEGQLKA
                     IGKLQGVIEFDLVGNILKVNDNFINVTGYSESEIVGKHHSMFVDATYKSSSEYNAFWE
                     KLGRGEAHEGVYKRIGKGGKEIWLQATYNPIIGIDGKPTKVVKYATDVTKYKLEEIEN
                     TGQLASINRAMGAIEFSMDGKVTKVNENFANLTGYSEKEIVGNHHSMFVEATYKSSAE
                     YKAFWDKLNRGEFDSGQYLRIGKGGKEVWLQATYNPILDLNGKPFKVVKFATDITEQK
                     NNAV"
     gene            complement(2015..2524)
                     /locus_tag="PKL42_08905"
     CDS             complement(2015..2524)
                     /locus_tag="PKL42_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNU67233.1"
                     /translation="MSMNTETVANTSNNKLKVAGGEYLTFVLGDEQYGLEILKVQEIR
                     GYDTVTQIANTPDFIKGVVNLRGKIVPIVDLRIKFKLGKVEYDEFTVVIILNLGGRVV
                     GIVVDGVSDVKALKEEQIRDVPSLVSNIDTKYIVGLATVEEQMFILVDIEQLMSSQEM
                     ALMDAVAVH"
     gene            complement(2564..>4553)
                     /locus_tag="PKL42_08910"
     CDS             complement(2564..>4553)
                     /locus_tag="PKL42_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNU67234.1"
                     /translation="NPVPVKIESIVNTPAATATDGLTHFNIVLPEISDKDASNLQEEL
                     SLLGEVATSKNAQGRYVLSLATDSTQADIVSICSFIVEPEDLVITLASAGTVAPATLE
                     TATPVADAGYGFFDDDIPTFASAGSSATVVNSATAPNTAASVNKTSKSNEGNKETVKI
                     HEEVGYGLFATEGKENVEEGYGFFAPFQPHPDATVATLIGDDTALTEANSGKAPAEKV
                     EVTQTETNATTVASAAAEKRNATRRESDKAPATTETSSIRVGVEKVDQLINLVGELVI
                     TQAMIDQRISALDPVQHESLINSVGQLTRNTRDLQESVMSIRMMPMDFVFSRFPRMVR
                     DLAGKLSKKVDFVTVGAATELDKGLIERIVDPLTHLVRNSVDHGIETPEKRRAAGKSE
                     TGTLTLSASHKGGSIVIEVTDDGAGLNRNKLLEKAAKNGLSVSENMTDAEVFNLIFAP
                     GFSTAEVVTDVSGRGVGMDVVKRNITAMNGAVEIRSALGFGTTISIALPLTLAILDGM
                     SVSLGNSVYVIPLNLIVETLQPRAEDIKTVTGEGRMVHVRGEYLPIIALHSLFNHHTD
                     ITDPTQGVLVLIEAEGKKSALFVDRLVGQQQVVIKSLETNYRKVQGVSGATIMGDGSV
                     ALILDVPAIIQMGQTANYITGAIPFANYQNKAVETQLI"
BASE COUNT         1174 a         1065 c          883 g         1431 t
ORIGIN      
        1 aatcttttta gcgctttcgt tgatggagct catagtagca accacttgac caaccacttc
       61 accgccttta atggcaacgc ctgaagcagc agcagccaac tggttggctt gtttagcgtt
      121 ctcagcattt tgttttacag tgcttgccaa ctcttccata ctagaagcgg tttcttctag
      181 gcttgaagct tgctgttctg tgcggcttga taaatcagtg ttgcctgaag agatttcacc
      241 tgcagctgtg ttaatggttt caccagcatc tcgtacctga acaatgattt ccgtcatttt
      301 gtccatcaga gcatttacac catcacaaag caaagcaatt gcgcccgttt taccttctaa
      361 cggaacacgg ctggttaaat cccctgactt tgcagcttcg ataacttctt gagtttctga
      421 aaccgcattt tgaagttaaa cagcgttatt cttttgctct gtaatatctg tcgcaaactt
      481 aacaacctta aatggtttac cattcaagtc gagaatcggg ttataagtgg cttgtagcca
      541 aacttcttta ccgcctttgc caattcgcaa gtattggcct gaatcaaatt caccgcggtt
      601 taatttatcc caaaatgctt tatattcagc actactttta taggtggcct caacaaacat
      661 actgtgatga ttgcctacaa tttccttctc gctataacca gtcaaattag caaagttttc
      721 attcaccttg gtcactttgc cgtccatcga gaactcaatt gcacccattg cacggttaat
      781 tgaagctaat tgaccggtgt tttcaatttc ttccaactta tacttagtaa cgtctgtagc
      841 atatttaacg accttggtcg gttttccatc aataccgata atcgggttat aagtggcctg
      901 caaccatatt tctttacccc ctttaccaat gcgtttatat actccttcat gagcttcacc
      961 acgacccagc ttttcccaaa aggcattata ttcactgcta gatttataag tagcatctac
     1021 aaacatactg tgatgtttac caactatttc actctcgcta tagccagtga cgttaataaa
     1081 gttgtcattc actttcaaaa tgttgccaac caaatcaaat tcaatgacac cttgtaattt
     1141 gccaatcgct ttcaattggc cttcaaaatc ggcatttttg attttttcag cagtaatgca
     1201 atatgaatac acgactactt tataagactt acctgcttca tctttaattg gcacataagt
     1261 cgattgaaac caatattctt tacggtcttt agaaagtcgg cgaatttgtc tggtttggct
     1321 aataccggcg ttgagttctt cccacatttt tttgtactct gggctttgcg caacttcagg
     1381 aggtagcaaa acacccacat gatgccccac taactcttct cttctataac ctagtggttg
     1441 cataaataaa tcattacact ctgtaatgtt gcctgccatg tcgcattcca tcacaccaaa
     1501 ggtttgatta agcgcttcat cttccccttg tagcacgagt gctcttgtta gctcagcggt
     1561 aatgtcggtt aaataactca ccactttaat gagttgatgc ttactgttaa tcacaggggc
     1621 gtagtagcct tgaaaccaaa catcttcacc ttttttattt ttaaatttga atttgcccgt
     1681 ttgcgtagta ccaccatgca ggttttccca aaagtcttcg tattctgggc gtgcgctctc
     1741 ggtgctgccc actagcacgc ggtgatgttc attcaacaca tctgattcac taaaccctaa
     1801 tgcttttaaa gcattctgat tgatggctat aatcgtgcct tttgcatcaa actcaagcac
     1861 acagcgcgct ttgttaattt cagcttttag ggcacgaaac tccgtgactt catcaatgat
     1921 ttttttagtg gcttgtgtgc cgctggctag tttattaata atagaagcaa acataataat
     1981 gaccttttct ttcttaatcg taatcgttta aaaattaatg aacagcaact gcgtccatga
     2041 gcgccatttc ttggctactc atcaattgtt caatatccac caaaataaac atctgttctt
     2101 caaccgtagc aaggccgaca atatatttgg tatcaatatt tgagactagc gatggcacat
     2161 cgcgaatttg ctcttctttg agcgctttaa cgtctgaaac gccatccacc acgatgccca
     2221 ccacgcggcc acccagattt aaaataatca ccacggtaaa ttcatcgtat tccactttgc
     2281 caagcttaaa cttgatgcgt aaatccacaa tgggcacaat tttgccgcgt aaattcacca
     2341 cgcctttaat aaaatctggc gtattggcga tttgtgtgac ggtgtcgtag ccgcgaattt
     2401 cttgtacttt caaaatttct aggccatatt gctcatcacc taaaacaaag gtgaggtact
     2461 cgccacccgc aacttttaat ttattgtttg aggtgttagc aacggtttct gtattcatgc
     2521 tcattgtgat gactccttaa ttttttaact tttagtgcta tgtttagatg agttgtgttt
     2581 ccactgcttt attttggtaa ttagcaaatg gaattgcccc tgtgatgtaa ttcgctgttt
     2641 ggcccatttg gataatggct ggcacatcaa gaattaaagc cactgaacca tcacccatta
     2701 tggtggcgcc agatacgccc tgcaccttgc ggtagtttgt ttctaagctc ttaatcacaa
     2761 cttgctgctg gccaaccaga cggtcaacaa aaagggcaga ctttttaccc tcagcttcaa
     2821 tcaacaccaa cacgccttgg gttgggtctg taatatcggt gtgatgatta aataaactat
     2881 gcagtgcaat aataggcaaa tattctccgc gcacatgtac catgcggcct tcgcccgtca
     2941 cggttttaat gtcctcagcg cgtggttgta gcgtttcaac aatcaaatta agcgggataa
     3001 cataaacact attacctaat gacacggaca tgccatctaa aatagccagc gtgagtggca
     3061 gtgcgattga aatcgttgtg ccaaagccaa gggccgagcg aatttctacc gcgccattca
     3121 tggcagtaat gttacgtttc accacgtcca tccccacacc acgaccagac acatcggtga
     3181 ccacttctgc agtagaaaaa cctggtgcaa atatgagatt aaacacttca gcgtctgtca
     3241 tattttcgct tactgataag ccatttttag cagccttttc aagtaacttg ttacggttga
     3301 gccctgcgcc atcgtcggtg acttcaatga caatactgcc acctttatgc gaggctgaaa
     3361 gtgtgagggt gccagtttct gacttaccag cagcgcgacg tttttcaggt gtttcaatgc
     3421 cgtggtctac actattgcgg actaaatggg tcaatgggtc aacaatacgt tcaatgaggc
     3481 ctttgtctaa ttcagttgct gcaccaacag tgacaaaatc gacctttttg cttaacttgc
     3541 cagccaaatc gcgcaccata cgtgggaagc gtgagaaaac aaaatccatt ggcatcatac
     3601 gaatcgacat gacagactct tgtaggtcgc gggtgttgcg tgtaagttgc cctacgctgt
     3661 tgatgagcga ttcatgctgt accggatcaa gcgcgctaat gcgttgatca atcattgcct
     3721 gggtaatcac gagttcgccc acaaggttaa tgagttggtc aaccttttct acacccacac
     3781 gaatggatga ggtttctgta gttgctgggg ctttatctga ttcgcgacgg gttgcgtttc
     3841 ttttttctgc ggctgcactg gctactgttg tagcattagt ctctgtttga gttacttcta
     3901 ctttttcagc tggcgctttg ccagagttcg cttcagtgag tgccgtatca tcaccaatca
     3961 gtgttgcgac cgtggcatct ggatgtggtt gaaatggtgc aaagaagccg taaccttctt
     4021 ctacattttc tttgccttct gttgcaaata aaccgtaacc gacttcttca tgaattttaa
     4081 cagtctcttt attaccttca tttgatttac ttgttttgtt gactgatgca gctgtgtttg
     4141 gtgcagttgc cgaatttact actgtggcac ttgaacctgc agaagcaaac gttggaatat
     4201 catcatcaaa aaaaccataa ccagcatctg ctactggggt agctgtttct aacgttgctg
     4261 gcgctacagt gccagcagaa gccaacgtaa tcactaaatc ttcaggctct acaataaaag
     4321 aacaaataga aactatgtca gcttgtgtgg aatcagtcgc cagacttaac acataacggc
     4381 cctgtgcatt tttgctggta gcaacttctc ccaagaggct taactcttct tgtaagttgc
     4441 ttgcgtcttt atctgatatt tcagggagta cgatattaaa gtgcgttaag ccatccgttg
     4501 cagttgctgc gggagtgttt acaatcgatt caatttttac aggcactgga ttg
//
LOCUS       DAOQSI010000158         4522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_20338_length_4522_cov_4.510857, whole genome shotgun sequence.
ACCESSION   DAOQSI010000158 DAOQSI010000000
VERSION     DAOQSI010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20338_length_4522_cov_4.510857"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..550
                     /gene="rnpA"
                     /locus_tag="PKL42_08915"
     CDS             146..550
                     /gene="rnpA"
                     /locus_tag="PKL42_08915"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNU67235.1"
                     /translation="MVSSYRLTKQAKMIKTDDFSSVFSFHKRVASAHLVMHYRPNHLA
                     LARLGLVVGKKTAKLAVSRNYMKRVLRELFRHKQHEVSHVDLIVRVQKPFNRSEYKQI
                     QQEFELLITKLNQKVILNAQTLSSVNAAPLLG"
     gene            554..772
                     /gene="yidD"
                     /locus_tag="PKL42_08920"
     CDS             554..772
                     /gene="yidD"
                     /locus_tag="PKL42_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNU67236.1"
                     /translation="MKIVSRFLVGLIKVYQVLLSPFFGQQCRFYPTCSHYAIEAIHKH
                     GALLGSYYTIRRLLRCNPWCEGGHDPIP"
     gene            821..2473
                     /gene="yidC"
                     /locus_tag="PKL42_08925"
     CDS             821..2473
                     /gene="yidC"
                     /locus_tag="PKL42_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNU67237.1"
                     /translation="MDTKRLILFVIFSMSILMLWDAWQRQSAPVATQQASHVAASSNP
                     TSAATSQAVDATVGTQEVVNGTFKLLTGQRINVTTDLYKADIETVGGDLRRLELLKHR
                     AADNDKTNFVLLDDAAKPMTYVAQTGLIGAELPTHHAVFSSTATSYQMQENQDKLEVR
                     LSWVSANGIAVDKVYTFHRNQYVIEVAYEIKNGSTNAISPEVYYQIVHDSESNQGSAL
                     MPTFTGGSYYTEAKKFNKLAFSDMAKEPLNITSNDGWVGLLQHYFVSAWIPKDGLARK
                     FYTEQLADNMYRIGSRSALGTIAPDASYSISARLFSGPQTKNDLLATAPGLEYTVDYG
                     WLTIVATPLFWVLSKIHGLVNNWGVAIILLTLLIKAAFYPLSAKSYRSMAQMRELAPR
                     LESMKQKFGDDRQKMQQAMMELYRTEKINPMSGCLPILIQIPVFISLYWMLLGSVELR
                     HAPFFGWIQDLSAIDPYYILPLIMGASMIIQTKLNPKPTDPIQAKVMTWMPVIFSIFF
                     FFFPAGLVLYWVVNNIVSIWQQWYINKNIHAETLAKKTGGKR"
     gene            2460..3809
                     /gene="mnmE"
                     /locus_tag="PKL42_08930"
     CDS             2460..3809
                     /gene="mnmE"
                     /locus_tag="PKL42_08930"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNU67238.1"
                     /translation="MANANAQDTIAAIATASGAGGIGIVRISGPAASAIAASILGTCP
                     APRHAAYLPFKLENDEPIDRGIAIFYPTPHSYTGEDVLELQAHGGTALMQLLLARCIS
                     LGARQAEPGEFTRRAYLNEKIDLAQAEAVADVINAATVEAAKSAARSLSGEFSQTINT
                     LLNRLIDLRMFVEACLDFPEEEIDFITQGRVAEKLEAITDALSVVFAKAKQGSLLREG
                     INVVLVGQPNVGKSSLMNALAGEEIAIVTAIAGTTRDTIKNAIQINGVPLHVIDTAGL
                     RETDDEVEKFGIARTWASLQTANIALLLVDAAHGITEAEKSILTRLPQEIAKIWVHNK
                     IDVSRETPKIVEIAQETHIHLSAKTGEGLELLKSQLLKLAGYRASGEGVFMARARHLE
                     ALQLVNVHLHEARIQLSSSELIAEELRLAQEALSSITGEFTPDDLLGEIFSKFCIGK"
     gene            complement(3879..4208)
                     /locus_tag="PKL42_08935"
     CDS             complement(3879..4208)
                     /locus_tag="PKL42_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2853 family protein"
                     /protein_id="HNU67239.1"
                     /translation="MADIQEHKATVAKYVKNLNTAALDGLAKNYALVLSNKDSQYVAA
                     TDEAERITVRENFLKKKLGLTNSDAELDASVEAVGAVLKDEHFKSRLAYYYILAEKYN
                     KLDLFIK"
BASE COUNT         1342 a          870 c          996 g         1314 t
ORIGIN      
        1 gtgattgcat cacgtcgtgc taagggccgt aaacgtttag gtctgtaatt ttagatcaaa
       61 cttttaacct gctacaatag gtggtgttaa aagtattggc aacattgatt aagcacgtta
      121 attttaaata cggtttaatt taataatggt gtcatcatat cggcttacga aacaagccaa
      181 aatgattaaa acggatgatt tttcatccgt ttttagtttt cacaagagag tggcatcagc
      241 gcatttagtc atgcactatc gacccaatca tttggcgcta gctcggttgg gtttagtggt
      301 aggaaaaaaa acagccaaat tggcggtaag cagaaactac atgaagcgtg tgttacgtga
      361 gttatttcgc cataaacaac atgaagtcag tcatgtggat ttaattgtac gtgtgcagaa
      421 accgtttaat agaagtgaat acaaacaaat tcaacaagag tttgaactgt taattacaaa
      481 attaaatcaa aaagtgatat taaatgcgca aacactttct tcagttaacg cagcaccatt
      541 gctaggttaa tttatgaaaa tagtctcgcg ttttttagtg ggtttaatta aagtgtatca
      601 ggtgttgtta agtccatttt ttggtcaaca atgtcgcttt tacccaactt gttcacatta
      661 cgcaatagaa gcaattcata agcacggtgc acttttaggt agttattata cgattcgccg
      721 tttgctacgt tgcaaccctt ggtgcgaagg tggacacgat cccatccctt aagctgttag
      781 aatatatcaa cgaaattttt acaaagatta cttatcaatc atggatacca agcgcttaat
      841 tttattcgtt atattttcaa tgtcgatatt gatgttgtgg gatgcatggc agcgccaaag
      901 tgcgccggta gctacccagc aagcgagcca tgttgccgca agtagcaacc cgacaagcgc
      961 cgcgacaagt caggcagttg atgcaacagt cggcacgcaa gaggttgtta atggcacatt
     1021 taagttgctt actggccaac gtattaacgt tacaactgat ttatataaag cggatattga
     1081 aacggttggc ggtgacttac gccgattaga attacttaag catcgtgcgg cagacaacga
     1141 taagacaaac tttgttttat tagatgatgc ggctaaaccg atgacttacg tagcgcaaac
     1201 agggttaatc ggtgcggaat taccaaccca tcatgcggtt ttcagtagca cggccacaag
     1261 ttatcaaatg caagaaaatc aggacaagct agaagttcgt ttgagttggg tgagtgcaaa
     1321 tggtatagcg gttgataaag tctatacctt ccatagaaat caatatgtta tcgaagtggc
     1381 gtacgaaatt aagaatggtt cgactaatgc gatttctcca gaagtctatt accaaattgt
     1441 gcatgacagt gaatcaaatc aaggatcggc cttgatgcca acgtttactg gtggctcata
     1501 ctacacggag gctaaaaaat tcaataagtt agcctttagt gacatggcta aagagccgct
     1561 gaatatcaca tcaaatgatg gctgggttgg gttgttgcaa cactattttg tgagcgcctg
     1621 gataccgaag gatggattag cgcgtaaatt ttatacagaa cagttagcag ataacatgta
     1681 tagaattggg tcaagaagtg cactcggtac catagcgccg gatgcaagct acagcatttc
     1741 agcgcgttta ttttctggcc cacaaaccaa aaatgatttg ctagcaacag cgccagggtt
     1801 ggaatacacc gttgattatg gttggttaac catcgttgca acgccattat tttgggtact
     1861 atccaaaata catggcttgg tgaataactg gggtgtggca attatattgc ttacactgtt
     1921 gattaaggct gcgttctatc cattatcagc aaaaagctac cgctcaatgg cgcaaatgcg
     1981 tgaattagca ccgcgcttag aatcaatgaa gcagaaattt ggcgatgatc gccaaaaaat
     2041 gcagcaagcg atgatggagc tttatcggac ggaaaaaatt aatccaatga gtggttgttt
     2101 gcccattttg attcaaattc cagtatttat ttcactttat tggatgttat tgggttctgt
     2161 agagttacgt catgccccat tctttggttg gattcaagac ttgtcagcca ttgatccata
     2221 ctatattttg ccgcttatta tgggtgcttc tatgattatt caaacgaagc tgaacccgaa
     2281 gccaaccgac cccattcaag ccaaggtgat gacatggatg cccgtcattt ttagtatatt
     2341 cttcttcttc ttccctgcgg gtttggtgtt gtactgggtt gtgaacaata ttgtttccat
     2401 ttggcaacaa tggtacatca acaaaaatat ccacgcagaa acattagcca aaaaaacagg
     2461 tggcaaacgc taacgcacaa gacaccattg ctgcaatcgc caccgcgagt ggtgctggcg
     2521 gcattggtat tgttcgtatc tctggccctg cggcaagtgc cattgcagcc agtattttag
     2581 gcacttgccc tgctccacgg cacgcagcgt atttaccgtt taaattagaa aatgacgaac
     2641 cgattgaccg cggcatcgct attttttacc ccacccccca ttcgtacact ggtgaagatg
     2701 tgcttgaact tcaagcgcat ggcggcacag cgctgatgca attattatta gcgcgctgta
     2761 tcagcttagg cgcgcgtcag gctgagccag gtgagtttac ccgccgtgct tacctgaatg
     2821 aaaaaataga ccttgcgcaa gctgaggcgg ttgccgatgt cattaatgca gcgacggtag
     2881 aagcggctaa aagtgcagcg cgttcgttat cgggtgagtt ttcgcaaacc atcaacaccc
     2941 tcttaaatag gctgattgac ttacgtatgt ttgttgaagc ctgcttggat tttccagaag
     3001 aagaaattga cttcattacc caaggccgag tggcagaaaa attagaagcc attactgatg
     3061 cgttaagtgt tgtctttgca aaagccaagc aaggcagctt gttgcgtgaa ggcatcaacg
     3121 tggtgttggt tgggcagccc aatgttggta aatcgagttt gatgaatgcg ttggcgggag
     3181 aagaaattgc gatagtaact gcaattgctg gcaccacacg cgacaccatt aaaaatgcca
     3241 ttcaaattaa tggcgttcct ttgcatgtca ttgatacagc aggcttgcgt gaaacagacg
     3301 atgaagttga aaaatttggc attgccagaa cttgggcgtc tctacaaacg gccaatatcg
     3361 cattattatt ggttgatgca gctcatggta ttacagaagc agaaaaatcg attttaacgc
     3421 gcttgccgca agaaattgcc aaaatttggg tacacaataa aattgatgtt tcacgtgaaa
     3481 cgccaaaaat tgtagaaata gcgcaagaaa cgcatattca tctttcggca aaaaccggtg
     3541 aaggtttaga actactaaaa tcgcagctgt taaaattagc aggatatcgg gcatcgggtg
     3601 aaggtgtatt tatggcaaga gcacggcatt tagaggcgct tcaattagtg aatgtacatt
     3661 tgcatgaggc tcgcattcag ctgtcttcat ctgagttgat tgccgaagag ttacgtttag
     3721 cacaagaagc gctgagtagt attacaggag aatttacccc ggatgatttg ctgggtgaga
     3781 tattttctaa attttgtatc ggtaaataaa agcttaagta aaaaggccat cgtgaagatg
     3841 gcctttttac ttccaaaaaa atgctgttta aacagtgatt atttaatgaa caaatcgagt
     3901 ttgttatatt tctcagcaag aatgtaatag taagcaaggc gagatttaaa gtgctcatct
     3961 tttaatacgg caccaaccgc ttcaactgat gcatctagct cagcatctga gttagttaag
     4021 cctaattttt tctttaaaaa gttttcacga acggtaattc tttctgcttc gtcggtagct
     4081 gccacgtatt gtgaatcttt atttgacaac actaatgcat agttttttgc taaaccatct
     4141 aatgccgcag tgtttaaatt tttaacatat ttagcaacgg ttgctttgtg ttcttgaata
     4201 tcagccattc ttctctcctc aaattagtat tatggaacga aaaccaaaca aatttttgaa
     4261 tcttattatc gcaacgttta agcataaaac acgtacttac tggtttttac accgtctcgc
     4321 tgacaatact ataggcttat cgtgatgtat ccgtcaatac ctgttctata cgtaggttat
     4381 ggcaaattgt aaacaatcgt gaaaaggaga aggaagacca taagcgccaa aaatgcatag
     4441 cacacaaagt attttctgct gactgaatag gaaacagcgc tatctttttt atgtttctag
     4501 gatatggtta aaaccttttg at
//
LOCUS       DAOQSI010000159         4477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_20642_length_4477_cov_4.615559, whole genome shotgun sequence.
ACCESSION   DAOQSI010000159 DAOQSI010000000
VERSION     DAOQSI010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20642_length_4477_cov_4.615559"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="PKL42_08940"
     CDS             complement(<1..581)
                     /locus_tag="PKL42_08940"
                     /inference="COORDINATES: protein motif:HMM:NF025149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4172 domain-containing protein"
                     /protein_id="HNU67240.1"
                     /translation="MNSGNHQKYIWQHAEWPQWLFNSAAFSQLLSQVNLERGRLLGSM
                     QALGFKLAEEASLKVLTSEVVKSSEIEGERLNPEAVRSSITRRLGIEAGGLVPSDKHV
                     DGVVEMVLDATRNCSAALTHERLFGWHAAIFPTGHSGMFKLTVAAYRDDAAGAMQVVS
                     GGIGHEKVHYEAPSAAVIQTEMTHFLDWFNNELG"
     gene            complement(728..812)
                     /locus_tag="PKL42_08945"
     tRNA            complement(728..812)
                     /locus_tag="PKL42_08945"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(776..778),aa:Leu,seq:caa)
     gene            874..1914
                     /gene="queA"
                     /locus_tag="PKL42_08950"
     CDS             874..1914
                     /gene="queA"
                     /locus_tag="PKL42_08950"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNU67241.1"
                     /translation="MRTLDFDFYLPEALIAQHPTKNRSASRLLKLDGNTGSIQDAMFT
                     NLLEALNSGDLLVLNDTLVIKARLFGEKLTGGKIEVMIERVVDAHHALAQIKASRAPK
                     AGSKLVIAGKVEAEVIGRQEDLFLLQFSDSHEVMDILDLHGSLPLPPYITHAADADDE
                     TRYQTVFAKHAGAVAAPTAGLHFDDTMLNQLKAKGVNIAFVTLHVGAGTFKPVKTELI
                     ADHKMHSEIYTIPQATIDLIAATRTNGGKVTAVGTTALRALESAAQHQALQAGQGETA
                     IFITPGYSFKVVDRLLTNFHLPKSTLLMLVSAFAGFENTMRAYQHAIEKQYRFFSYGD
                     AMLIERKRNLTD"
     gene            1997..2845
                     /locus_tag="PKL42_08955"
     CDS             1997..2845
                     /locus_tag="PKL42_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009852446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67242.1"
                     /translation="MATTLTCFAGSLQAEILPQAELALKFGLANKSTTRPMTVAYIPS
                     HKKYYIADGGLAPMGSETEAPISKSLIHSYDETGKYVNSSRPGYDNRSIYFNTNTNKL
                     ETITYNVSSDVGFSPNTGIYAINLTETGELKDTSDEISQFNPAFGHSGTMPSYDAANQ
                     RYFAKQGRSDKVFIVDPTKREKIGEIKLDLAKAGVAHDDISDLFAAYTGIQGEELLLL
                     DIDHKAILVFSIDGQYVGKSELPKELKLRANNHYTGLGYANQLFFVFHEPEGEFGTYY
                     AFKVQK"
     gene            complement(2919..3422)
                     /locus_tag="PKL42_08960"
     CDS             complement(2919..3422)
                     /locus_tag="PKL42_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HNU67243.1"
                     /translation="MSKLTALALSIAVLGGVWAFLALNPLAGVALVWVGFIAWGCYFH
                     TGGDGAALQKTIAGMSFGAVLAGIALYLVSSGTLGGLGALAAPLIIAVTVFPLVAVAG
                     FNLLSTVPANVYGYAATAGLALTASTAGNVTAADFSNPVLLVIVSTVIGAIFGMLSGK
                     LAGMLGK"
     gene            complement(3629..4300)
                     /locus_tag="PKL42_08965"
     CDS             complement(3629..4300)
                     /locus_tag="PKL42_08965"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNU67244.1"
                     /translation="MRKAFQHLVLLLLLVSGSTFAGSQIEEPLSNSVKAMMQRSISDK
                     AAPRLVFATPEEGQAWLNEMSERLEKRIPNAVYREDVLRTVHYEATRAGLDPQMVLGL
                     IQVESGFKKYAVSSVGARGFMQIMPFWVKSIGDKDHNLFQLRTNLRYGCTILRHYLDI
                     ERGDLYRALGRYNGSLGRPQYPNLVLGAWRKHWHYTPQLGASSIKVSENAQSTSSDTL
                     KTAQN"
     gene            complement(4324..4461)
                     /locus_tag="PKL42_08970"
     CDS             complement(4324..4461)
                     /locus_tag="PKL42_08970"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HNU67245.1"
                     /translation="MGIPHRVVIGERGLVDNMVEYKARTCAEAQSVSLAEIVTFVQKV
                     S"
BASE COUNT         1305 a         1109 c          913 g         1150 t
ORIGIN      
        1 cccagttcat tattaaacca atctaaaaaa tgtgtcatct cagtttgaat tactgcggct
       61 gaaggtgctt cataatgcac tttttcatgg cctattccac ctgaaactac ttgcattgca
      121 cctgctgcat catctctata ggctgcaacg gtgagtttga acatgccact atgacctgta
      181 gggaaaattg ctgcgtgcca gccaaacaaa cgctcatgtg ttaatgctgc gctgcaattg
      241 cgtgtagcat ctaacaccat ttccaccaca ccatcgacat gcttatccga aggcactaaa
      301 ccgcctgcct ctatgcctag ccttctggtt atggatgagc gtactgcttc agggttgagt
      361 cgttcacctt ctatctcact cgatttaact acttcactgg tcaacacttt aaggctagcc
      421 tcttcagcca gtttaaagcc caacgcctgc atagaaccaa gcaagcgacc acgctcaaga
      481 ttcacctgtg ataatagttg cgaaaatgcc gctgaattaa acaaccattg cggccattca
      541 gcatgttgcc agatatattt ttgatgatta ccgctattca tgcggtaatc attgcacgtt
      601 attaccgcac tgtcaacaat caccgctttt tttgcggtga tatactaaaa ccattaccgc
      661 atagccgtta ggatgtaata gacgtaaaaa agggcactct gatcaagtgc ccagctttaa
      721 accatattgg tgcccggggc cggaatcgaa ccggcacgca ttgctgcgcg ggattttgag
      781 tcccgtgcgt ctaccaattc cgccacccgg gcatttggga ctatacatta ctgtataatt
      841 gcaacaccgc tattatagca agctcttaga attatgcgaa cccttgattt cgatttttat
      901 ttaccagaag cacttattgc acagcatcct accaaaaaca gaagtgccag ccgcttgcta
      961 aagctcgatg gcaacaccgg aagcattcaa gatgcgatgt ttacaaactt attagaggcg
     1021 ctcaacagtg gcgacttact agtactcaat gatacgctcg tgattaaagc gcggctcttt
     1081 ggcgaaaaac tcaccggcgg aaaaattgaa gtgatgattg aacgcgtggt ggatgctcat
     1141 catgcgcttg cgcaaattaa ggcatcacgt gcgcctaaag ccggaagcaa gcttgtgatt
     1201 gccggcaaag ttgaagctga ggtaattggg cggcaagaag atttattttt actgcagttt
     1261 agtgacagtc atgaggtgat ggatattcta gacctgcatg gcagcctacc cctgcctcct
     1321 tatattacgc atgcagcaga tgcagatgat gaaacgcgct accaaaccgt atttgccaag
     1381 catgctggcg cagtggctgc acccaccgct gggctacatt ttgatgacac catgctaaat
     1441 cagcttaaag ccaaaggcgt caacattgcg tttgtgacat tgcatgtagg cgccggcaca
     1501 tttaagcccg ttaaaaccga gttgattgcc gaccacaaaa tgcatagtga aatctatact
     1561 attccacagg ctacgattga cctcattgcg gccacccgca ccaacggcgg caaagtaacg
     1621 gccgttggca caaccgcgtt gcgcgcgctt gaaagcgctg cacaacacca agcattacaa
     1681 gcgggacaag gtgaaactgc catttttatc acccctggat attcttttaa ggtggttgat
     1741 cggctactca cgaattttca tttgcctaaa agcacgctgc tgatgctggt atctgccttt
     1801 gctggatttg aaaatactat gcgggcgtat cagcatgcta ttgaaaaaca gtaccgtttt
     1861 tttagttatg gtgatgccat gctgattgaa cgtaaacgca acttaacaga ctaaacaaca
     1921 caataaatat agaacaatgc ctttttggag ataactaccc atgctttttg ctactcataa
     1981 aaacttgctg tgcactttgg cgactacgtt aacttgcttt gcaggaagcc tacaggccga
     2041 aattttgccg caggctgaac tcgccttaaa gtttggtctg gcgaacaaat ccaccacgcg
     2101 ccccatgacg gtggcctata ttcctagcca taaaaaatat tacattgcgg atggtggctt
     2161 ggcgccgatg ggcagtgaaa ccgaagcgcc aatttcaaaa tcgctgattc atagctatga
     2221 tgaaaccggc aaatatgtaa actcttccag acccggttat gacaaccgct cgatatactt
     2281 caacaccaac accaataaac ttgaaaccat tacctacaat gtttcgagcg acgttggttt
     2341 ttcacccaat acgggcattt atgcgattaa tctgactgaa actggagagc ttaaagatac
     2401 ttcagatgaa atctcgcagt ttaatccagc ctttggtcac tcaggcacca tgccgagcta
     2461 cgatgcggcc aatcaacgct attttgcaaa acaaggccgt agcgacaaag tgtttattgt
     2521 agacccgacc aaacgtgaaa aaattggtga aatcaagctg gatttagcaa aagccggggt
     2581 agcccatgat gacatcagcg acttatttgc cgcctacacc ggcattcaag gtgaagagtt
     2641 gctacttctc gatattgacc acaaggcaat ccttgttttt agcatcgatg ggcaatacgt
     2701 gggcaaaagt gaattaccga aagaattaaa actacgcgcc aataaccact acaccgggct
     2761 tggttatgcc aaccaactat tttttgtatt tcacgagcca gaaggtgaat ttggtactta
     2821 ctatgcattt aaagtgcaaa aatagcatta ctttcgttta gccaataaaa aaccgccaaa
     2881 gatggcggtt ttttattggt gaataatatt gtcgcttatt atttacccaa catgcctgcc
     2941 agctttcctg aaagcatgcc gaaaattgca ccaatcactg ttgaaacaat caccaataat
     3001 actggattgc tgaagtctgc tgcagtcaca ttgcccgcag tactcgctgt tagcgccaaa
     3061 ccagccgttg cagcataacc atagacattt gccggaacgg tagataacaa attaaaacct
     3121 gcaacagcca ccaacgggaa cactgtcaca gcaataatca gcggcgcagc taaagcacct
     3181 aagccaccaa gcgtaccaga actaactaaa tacaaggcaa taccggctaa aacagcccca
     3241 aaactcatac cagcaattgt tttttgcaat gcagcaccat cgccacctgt gtggaaataa
     3301 caaccccaag cgataaaacc aacccaaacc aaggcaacgc ccgcaagtgg atttaacgct
     3361 aaaaatgccc acacaccccc caaaacagca atacttaatg caagtgcagt taatttcgac
     3421 atacttttct cctaaattta aaatttttaa tgaatactaa gatgatgcga agaacagcgc
     3481 gtatgcagtg gttttgttat gaaatcaccc ttttgcatgt ataacgctag gggaatactt
     3541 aatgattttg cttgaaatgt aaacttatgt acgccgcttt cattcacccg tttgggtgaa
     3601 tgacagagta aaaacgacca aaaaagcact aattttgtgc agttttcagt gtatcacttg
     3661 aagttgattg cgcattttca ctgactttga tggatgaagc gccaagctga ggcgtgtaat
     3721 gccagtgctt gcgccaagcc cctaacacta agttaggata ttgcggacgg cctagactgc
     3781 cgttatagcg acccagcgca cggtataaat cgccacgctc aatatctaga taatgccgaa
     3841 gaatcgtgca gccataacgc agattggtgc gcaactgaaa taaattatgg tctttatcac
     3901 caatgctttt cacccaaaac ggcataattt gcataaaacc acgcgcaccc accgatgaaa
     3961 ccgcatattt tttaaaaccg ctttccactt gaattaaacc cagcaccatt tgcgggtcta
     4021 agccagcgcg cgtggcctca taatggacgg tgcgtaatac atcttcacga tacaccgcat
     4081 ttgggatgcg cttttctaag cgctctgaca tctcattcaa ccatgcctga ccttcttctg
     4141 gcgtggcaaa taccagccta ggcgcggctt tatcactaat actgcgctgc atcatggctt
     4201 ttacactatt tgatagcggc tcttcaattt gagagcctgc aaaagtgctg cctgatacca
     4261 gtagtaaaag taatactaaa tgttgaaacg ctttacgcat gttaaataaa accaaccctt
     4321 tgtttatgac actttttgta caaaagtgac gatttctgct aaagaaactg actgcgcctc
     4381 agcacatgtc cgcgccttat attccaccat attatcaacc aacccacgct cgccaatcac
     4441 cacgcggtgc gggattccca ttaaatcagc ctcggca
//
LOCUS       DAOQSI010000160         4442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_20906_length_4442_cov_5.807841, whole genome shotgun sequence.
ACCESSION   DAOQSI010000160 DAOQSI010000000
VERSION     DAOQSI010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20906_length_4442_cov_5.807841"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PKL42_08975"
     CDS             complement(<1..548)
                     /locus_tag="PKL42_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNU67246.1"
                     /translation="MKITDTFRFATNAATAAPLRAGLLVLAMSIGVAAVVMLTALGDG
                     ARRYVIDQFSAIGTNLVIVLPGRSETRGFNPANLVTSTPRDLTVEDANALLRLPAVKR
                     IAPILVATTEINAAGKLREAMLLGTNDEYINVRQTKIGLGRFLSKDEVGRGSAEVVLG
                     AGIRREIFGAENPLGKTVRVGD"
     gene            complement(567..1322)
                     /locus_tag="PKL42_08980"
     CDS             complement(567..1322)
                     /locus_tag="PKL42_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNU67247.1"
                     /translation="MPDESPTSTLISLNGINRTFYLGDSKVHALRDVNLTITRGEYIA
                     VMGPSGSGKSTLLNLIGLLDRPDEGTYMLEGRDVTTLGADELAAVRSERIGFIFQSFH
                     LVPRLTAAANIELPLVLSGFPTAERAKRVSQALQEYGLTDRANHRPNELSGGQRQRVA
                     IARATILRPAVLLADEPTGNLDRVTGEEVVKLLEKLNAQGMTLIVVTHDQALGARAHR
                     QIRMVDGGIESDVINSEIATNDAPEAARSPSTS"
     gene            complement(1329..2501)
                     /locus_tag="PKL42_08985"
     CDS             complement(1329..2501)
                     /locus_tag="PKL42_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNU67248.1"
                     /translation="MIRRILIVLFVIAGIGTAFWWFTRPKPVSVALVEVGRGMVEASV
                     ANTRAGSVEACLRTRLSPISGGRIAFLGVKKGDHVKKGQVLLRLWNDDQQAQSNLALA
                     QVTTSKKRIAEACAMAENAEREAARMAELRAKGFISKSGEEKSRYEAQSRRAACDAAR
                     ADVNQAQARVKVTQVEQNRTVLVAPFDGIVANIVGELGEYTTPSPPGVATPPAIDLID
                     DTCVYIEAPMDEVDAPKIHIGQAARVSLDALPNQPLAGHVKRVAPYVVAVEKQARTVD
                     VEVSLDNPEDGKKLLVGYSADVEIVLESRNDTLRVPTTTLLEGNKVLVYQPATQTLEA
                     RAIKTGITNWEYTEVLEGLKAGDRIVTSLEREGVKAGIKVTPENGSDSAADKIKAK"
     gene            complement(2634..3206)
                     /locus_tag="PKL42_08990"
     CDS             complement(2634..3206)
                     /locus_tag="PKL42_08990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNU67249.1"
                     /translation="MKIQNIKNSQAYRLLNHGPTILVSSSFNGKQNIMAAAWNLPLDF
                     DPPKICVVIDKKTYTRELIEASGTFAINVPCVAQVDLVTKLGSYSGRELQGTDKFAEN
                     QLQTFAAKHIAAPLLAGCVAWLECKIIPEPHNQNTYDLFIAEVVAAYADERVFSDGHW
                     HFDGYDELRTIHHIAGGAFFATGAAIQSTI"
     gene            complement(3203..4237)
                     /locus_tag="PKL42_08995"
     CDS             complement(3203..4237)
                     /locus_tag="PKL42_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67250.1"
                     /translation="MAEVVEMSVCTVYLPDSLTLENTIRFSNHLHDLPEADEYIFDFD
                     ALNWIEPFSLLLLSSEIQRCRDKVQQKRFSAVNHKTKTYAGHMGFFKAFGCDFGKKPS
                     EAVGNSRYIPITIYDSAEIQKAAYESSEAVGQFIENIAANISHVLLRTYDGELFDTFT
                     YSIREIIRNVIEHSNSGQFGFCAQYWPNKNQVELALLDRGIGIKKGLSSNPHLNINSD
                     HEALNLALMPGVSGKAFKGSKQNKDDNWANSGFGLYMTSRLCREGGSFYIASGNTSLM
                     LSEQNKDYMDTPFQGTALRLILNTNRIKSLNKMLEQYRGEAEKIKSHFSAENFSASTA
                     SRMLTKDFKT"
     gene            complement(4225..>4442)
                     /locus_tag="PKL42_09000"
     CDS             complement(4225..>4442)
                     /locus_tag="PKL42_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HNU67251.1"
                     /translation="EDGSERDFLEDINPNSVTTIKAQLEASLKDAKPAESFQFERHGY
                     FVADRKESVAGKPVFNRTVTLKDAWQK"
BASE COUNT         1106 a         1129 c          977 g         1230 t
ORIGIN      
        1 ctgtcgccca cccgcacggt tttaccgagc ggattttctg caccaaaaat ctcgcgacga
       61 atccccgcac ccagcaccac ttctgccgaa ccacgcccaa cttcatcttt tgaaagaaag
      121 cgcccaagcc caatcttcgt ctggcgaacg ttaatgtatt catcattggt gcccagcagc
      181 atggcttcac gcaactttcc tgcggcattg atttctgtgg tggccaccaa aataggcgca
      241 atacgtttga ctgctggcaa cctgagcaat gcgttggcat cttctaccgt taagtcgcgc
      301 ggtgtgctgg tgaccaaatt ggcaggatta aatccgcgcg tttcagaacg gcccggcaag
      361 acaatcacca agtttgtgcc gattgcagaa aattggtcaa tgacataacg ccgcgcgcca
      421 tcacccaagg ccgtgagcat caccaccgcc gccacgccaa tcgacattgc cagcaccaat
      481 aagcccgcgc gcaacggcgc ggcagtggcg gcattggttg caaagcgaaa ggtatcggta
      541 atcttcaaaa ccaaatcctt aacgacttaa cttgtcgatg gtgagcgagc agcttctggc
      601 gcatcattgg ttgcaatttc gctgttgatc acgtcgcttt caatgccgcc atcaaccatt
      661 ctgatctgtc gatgcgcccg cgcgccgagc gcttggtcat gcgtcaccac aatcagtgtc
      721 atgccttgcg cattaagttt ctcaagcagt tttacgactt cttcccctgt cacacggtca
      781 aggttgcctg ttggttcgtc tgccagcaac accgctgggc gcaaaatcgt tgcccgcgcg
      841 atggccacgc gctgtcgctg cccacctgaa agttcattag ggcgatgatt tgctctgtct
      901 gttaagccat attcttgcag tgcctgcgaa acacgcttag cgcgctcagc ggttggaaag
      961 cctgacaaca ccaaaggcaa ctcaatattt gcggcagcag tcaggcgtgg gactaaatga
     1021 aaactctgaa aaataaaacc gattcgctcg ctacgcactg cggctaattc gtctgcgcca
     1081 agcgtggtca cgtcgcggcc ttccagcata taagtgcctt catcggggcg gtcgagcaag
     1141 cctattaaat tgagtaaggt tgatttgcca gagccggatg gccccatcac ggcaatgtat
     1201 tcgccacgcg ttatcgtcaa attcacatcg cgcagggcgt gcactttgct gtcccctaaa
     1261 taaaacgtgc ggttgatgcc atttagcgaa attaaggttg aagtcgggct ttcgtcaggc
     1321 ataaacgatt atttcgcctt gattttatca gcggcactat cgctgccatt ttcaggcgtc
     1381 actttgatgc ctgcttttac accttcgcgc tcaagggatg tcacaatacg gtcgcccgct
     1441 ttgaggcctt ctagcacttc ggtgtattcc caattggtaa tgccagtttt aatggcgcgc
     1501 gcttctagcg tttgcgtggc tggttgatac accagcactt tgttcccctc aagcagcgtt
     1561 gtggtgggta cacgcaaagt atcattgcgg ctttcaagca caatctcaac atcggcgctg
     1621 taacccacca gcagtttttt tccgtcttca ggattatcta acgacacttc aacatctacc
     1681 gtgcgggctt gtttttcaac cgctaccaca taaggtgcaa cgcgttttac atggccggcc
     1741 aatggctggt taggcagcgc atctaaagaa acccgcgccg cttggcctat atggattttg
     1801 ggcgcatcta cttcatccat cggcgcttca atatacacac aggtatcatc aatcaaatct
     1861 atggcgggcg gcgtagcaac accggggggt gatggcgtgg tgtattcacc caactcgccc
     1921 acaatattgg ccacaatgcc atcaaacggt gcaaccaaaa cggtgcgatt ttgctctact
     1981 tgcgttactt ttacccgcgc ttgcgcctga ttcacatctg ctcgcgcagc atcgcaagca
     2041 gcgcggcgcg attgtgcctc gtaacgtgat ttttcttcac cactttttga aataaaacct
     2101 tttgctcgca gttcagccat gcgcgcggct tcacgctcgg cattttcagc catggcacac
     2161 gcctcggcaa tgcgtttttt actggtggtt acttgagcaa gtgccaagtt actttgcgct
     2221 tgttggtcat cgttccacaa gcgtagcagt acttggcctt ttttaacatg gtcacctttt
     2281 tttacgccca aaaacgcaat gcgaccgccg ctgatgggcg ataatcttgt gcgtaaacaa
     2341 gcctctacac taccagcgcg cgtgttggca acgctcgcct ccaccatgcc gcgccctact
     2401 tcaaccaagg caacgctaac gggcttgggt cgtgtaaacc accaaaatgc agtgccgatg
     2461 ccggcaatga cgaaaagaac aatcagaatg cgtcgtatca tgtttgaata aggctttcac
     2521 ctgtaaaggg ttagtgtaat gatactgtta aacgctagca ttgagggcgc tctcagcaga
     2581 gtttacgtgt tcgcccttag attgaacgcg gtaggtgatg ccgctgaaaa aagttatatt
     2641 gttgactgaa tggcagcacc cgtggcaaaa aacgcgccac ctgcaatgtg atgaatggtg
     2701 cgcagctcat catatccatc aaaatgccaa tgcccatctg aaaaaacgcg ttcatccgca
     2761 taagcggcca ccacttctgc aataaataaa tcgtaagtgt tttggttgtg cggctcgggg
     2821 attattttgc actctagcca agccacgcag cccgccagca agggcgcggc aatgtgcttg
     2881 gcggcaaagg tttgtagctg attttctgca aatttatcgg tgccttgcaa ctcgcgccca
     2941 gaatatgagc ctagcttggt gactaaatca acctgcgcca cgcacggcac attaatggca
     3001 aatgtgccag aggcctcaat caactcacgc gtataggttt ttttatcaat caccacgcaa
     3061 attttaggtg ggtcaaaatc gagtggcaaa ttccacgccg ccgccataat gttttgtttg
     3121 ccgttaaaac tggatgaaac aaggatggtg gggccgtggt ttaggaggcg gtaggcctga
     3181 ctatttttta tattttgaat cttcaagttt tgaagtcctt agtcaacatt cgagaagctg
     3241 tagaagcgga aaaattttct gctgaaaaat gagattttat tttttcagcc tctcctctat
     3301 attgctctag catcttgttt aaagatttga ttcgatttgt attcaaaatt aaccttaatg
     3361 ctgttccctg aaaaggtgta tccatataat ctttattctg ctctgaaagc attagactag
     3421 tattaccact ggcaatgtaa aaactgcctc cttccctaca tagcctacta gtcatataaa
     3481 gtccaaaccc agagtttgcc caattatcat ctttattctg ttttgacccc ttaaatgctt
     3541 ttccagaaac tcctggcata agtgctaggt ttagtgcttc gtggtcactg ttaatgttta
     3601 gatgaggatt tgaggataaa ccttttttaa taccaattcc tctgtctaat agtgctagtt
     3661 caacttgatt tttgtttggc caatattgag cgcagaatcc aaattgcccg gaattactat
     3721 gctcaattac attacgaata atttctctta ttgaataagt aaatgtatca aatagttctc
     3781 catcataagt tctcaatagc acatgcgaaa tgttggcagc aatgttttca ataaattgac
     3841 ctacagcctc tgagctttca tatgcagctt tttgtatttc agctgaatcg taaattgtta
     3901 ttggaatata acgggaattt ccaaccgctt cacttggttt tttaccaaaa tcacatccaa
     3961 aggccttgaa aaatcccata tgtcctgcat aggtcttagt cttatgatta actgcagaaa
     4021 acctcttttg ttgaacttta tctctacagc gctggatttc gctacttaac aataaaagtg
     4081 aaaagggttc aatccaattc agcgcgtcaa aatcaaaaat atactcgtca gcctcaggta
     4141 aatcgtgcaa gtgattagaa aatctaattg tattttccag cgttagagaa tctggcaaat
     4201 aaacagtaca tacactcatt tcaactactt ctgccaagca tccttcaaag tcaccgttct
     4261 attaaaaaca ggcttgcctg ctacgctctc ttttctatcg gccacaaagt agccgtggcg
     4321 ctcaaattga aagctctcgg cgggttttgc gtcttttagg ctggcttcta attgcgcttt
     4381 aatggtggtg actgagtttg ggttaatgtc ttctaaaaag tctcgctcac ttccatcttc
     4441 at
//
LOCUS       DAOQSI010000161         4407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_21158_length_4407_cov_5.311811, whole genome shotgun sequence.
ACCESSION   DAOQSI010000161 DAOQSI010000000
VERSION     DAOQSI010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21158_length_4407_cov_5.311811"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..447)
                     /locus_tag="PKL42_09005"
     CDS             complement(31..447)
                     /locus_tag="PKL42_09005"
                     /inference="COORDINATES: protein motif:HMM:NF024750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HNU67252.1"
                     /translation="MARIKLTYGRINDFTCISGTQSFLWDTDSPGLAVRATTNGSKSY
                     IHQGKLNGRDVRITIGNVKSWDIDNARQESRKFQTQLDSGIDPRQERADKVIHSEARR
                     VRDKSLNNNLLAPWEEYIQENTAKIRDPWVLNCTQN"
     gene            806..1159
                     /locus_tag="PKL42_09010"
     CDS             806..1159
                     /locus_tag="PKL42_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNU67253.1"
                     /translation="MSIYHEIEAKDLFNLMAEQPLLLVDVRNDDEVARGIIPGAIHVP
                     LAMLPVQYEPLTKVETVVFYCHSGVRSAHAAAFITSKGCKNVYNLVGGILAWGKAGYP
                     LIAQNSATPAEGENK"
     gene            1159..1392
                     /locus_tag="PKL42_09015"
     CDS             1159..1392
                     /locus_tag="PKL42_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNU67254.1"
                     /translation="MEFDKLVDATGLNCPLPILRCKKGLSEINEHQILKVISTDAGSV
                     KDFNAFCVQTGHELLQLDSDNGTYTFYIKKREN"
     gene            complement(1562..>4407)
                     /locus_tag="PKL42_09020"
     CDS             complement(1562..>4407)
                     /locus_tag="PKL42_09020"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNU67255.1"
                     /translation="TTQNTTPPNQESAQGLDKSFDPKTIESKWYAFWESKGYYAAGLN
                     PEVKDNFCILLPPPNVTGTLHMGHGFNQTLMDALTRYHRMRGDNTLWQPGTDHAGIAT
                     QIVVERQLDAQGVSRHDLGREKFLEKVWEWKAYSGGTITQQMRRLGTSPDWSRERFTM
                     DAGLNKVVTETFVRLYNEGLIYRGKRLVNWDVKLGTAVSDLEVVQEEEDGFMWHINYP
                     LCSGEGHLTVATTRPETMLGDVAVMVHPEDERYAHLIGKNVKLPLCDREIPIIADDYV
                     DKAFGTGVVKVTPAHDFNDYAVGQRHKLPMIEMLSLSGLVNENAPEKYRGLTTIAARK
                     LVVADLEENGYLVKTEKHKLKVPRGDRTGVIIEPMLTDQWFVAMSKPAADGKSITQKA
                     LDVVASGEIKFYPENWVNTYNQWLNNIQDWCISRQLWWGHQIPAWYSDSGKVYVAKDE
                     AGAKVLAAKDGYTGNLVRDNDVLDTWYSSALWPFSTLDWTGDDGTDAQNQALQQYLPS
                     SVLVTGFDIIFFWVARMVMMTTHITGKIPFKHVYVHGLIRDAEGQKMSKSKGNVLDPI
                     DLIDGIDLNALIAKRTTGLMNPKDAEKIEKRTRKEFPEGIAAYGTDALRFTFAALASP
                     GRDIKFDLQRCDGYRNFCNKLWNATRFVLMNTEGQDNGFDAASCGEGGRKFSQADFWI
                     VSKLQRTEAEVEKAFEDYRFDLAAKAIYEFVWDEYCDWYLELAKVQIQNGTEAEKRAT
                     RRTLLRVLETILRLAHPIMPFITEEIWQTIAPMTGVNAPSIMLQPYPKSQPDKLDEAS
                     EAWVAQLKQMVDTCRRLRGEMSISPATRVPLIATGNAETLTAYAPYLKALAKLEEVAI
                     VAELPEADAPVMLVDDFKLMLKVEIDVAAEKERLGKEIARLENEINKANGKLNNESFV
                     ARAPAAVVEQEKARVAEFSASVDKLKNQFAKLK"
BASE COUNT         1107 a         1050 c          938 g         1312 t
ORIGIN      
        1 tttctagtgc aatttatatc gttaggtggg tcaattctga gtgcaattca acacccatgg
       61 gtctctaatc tttgcagtgt tctcttggat gtactcttcc catggcgcca gcaagttatt
      121 gttcaatgac ttatcacgaa cacggcgagc ttcactatgt attaccttat cagccctctc
      181 ttggcgtggg tcaatgccgc tatctaactg agtttgaaat ttgcgcgatt cttggcgtgc
      241 attatcaata tcccacgact tcacatttcc tatggtgatg cgaacgtctc taccattcaa
      301 cttgccttga tgtatgtatg acttggaacc gtttgtagtt gcacgaactg ctaaccccgg
      361 tgagtcagtg tcccaaagaa aagattgtgt gccagagatg caagtaaaat catttatcct
      421 gccgtatgta agtttaattc tagccatatt agcgccccat attggttaca catctatttc
      481 atgtaagcgc catgtaagcg ccatgtaagc gaaatgatag aatacgtatt aactttaatc
      541 aacacgtaat tttggataaa atgtgctaat atactgtttt tattagttat ctgataatat
      601 tatcaacact actcaacatg gttaaatata cttattggcg gattgaaaat ccgcgtgtcg
      661 gcagttcgat tctgcccccg gccaccagta tttttaagag ttctcttaac ctacaaatac
      721 ttctttggct tagcttctga cactttttaa tgcagtctag ccacataatt gctattattt
      781 cacttagtta aaattgattg ttcaaatgag catttatcac gaaattgaag caaaagactt
      841 attcaacctg atggctgagc agccgttgtt attggtggat gtgcgtaatg atgatgaagt
      901 tgcgcgcggc attattcctg gtgcaattca tgtgccatta gcaatgttgc ctgtgcaata
      961 tgagccttta accaaagttg aaacggttgt attttattgt cacagcggcg tgcggtcagc
     1021 gcatgcggcc gcttttatta ccagcaaagg ctgtaaaaat gtttacaact tagtgggcgg
     1081 cattcttgcc tggggcaagg cgggctaccc actcatcgcg cagaatagcg cgacaccagc
     1141 agaaggggaa aacaaataat ggagttcgac aaacttgtgg atgcaacagg cttaaattgc
     1201 ccgctgccaa ttttgcgctg caaaaagggc ttgagcgaaa taaatgaaca tcaaatttta
     1261 aaagtaattt ctaccgacgc aggttctgtg aaagatttta atgcattttg tgtgcaaact
     1321 ggccatgagc ttttacagtt agattcagat aacggcacct atacttttta tattaaaaag
     1381 cgcgaaaatt aataaaaaaa tagacgcttt taacgccgag cgaaatttgc tgccctgcga
     1441 gcctctattt acaaggcttc cagagcatct cataagggtt gtatcaaata tggcttatct
     1501 tttagcaata aaaaaggcac ttttagtgcc ttttttattt gtcatatttt ccgcttcgct
     1561 tctacttcaa ctttgcgaat tgatttttaa gtttatctac actggcactg aattctgcta
     1621 cccgcgcttt ttcttgttcc accacagcgg cgggcgcccg tgcaacaaaa ctttcgttat
     1681 ttaatttgcc attggctttg ttgatttcat tttctaagcg cgcaatttct ttgcctaaac
     1741 gctctttctc tgctgcaacg tcaatttcaa cttttaacat gagtttaaag tcatccacca
     1801 acatcacagg tgcatccgcc tcaggcaatt cggccacaat cgcaacttct tccagtttag
     1861 ccaaggcttt taaataaggc gcataggccg ttaatgtttc ggcattacct gtggcaatca
     1921 acggcacacg tgtggcgggc gaaatgctca tttcaccgcg taaacgacgg caagtatcta
     1981 ccatttgttt gagctgtgcg acccacgctt ctgaggcctc gtccaattta tcgggctgcg
     2041 attttgggta tggttgcagc atgatgctgg gcgcgttcac gcccgtcatt ggcgcaatgg
     2101 tttgccaaat ctcttcggta ataaatggca tgatggggtg tgctaatcgt aaaatcgtct
     2161 caagcacgcg taatagtgtg cggcgtgtcg cgcgtttttc agcctctgta ccattttgaa
     2221 tttgcacttt ggcgagctct aaataccagt cgcaatattc atcccagaca aattcataaa
     2281 tggcttttgc agctaagtca aagcgataat cttcgaatgc tttttcaact tcagcttcgg
     2341 tacgctgtag tttggatacg atccagaaat ccgcttggct aaatttacgc ccgccctcgc
     2401 cacagctggc cgcatcaaaa ccgttatcct gaccttctgt gttcatcagc acaaagcgcg
     2461 tggcattcca cagtttattg cagaaattac ggtagccgtc acagcgttgc aaatcaaact
     2521 taatatcacg cccagggctg gcaagtgcgg caaaggtaaa gcgcaaggca tcggtgccat
     2581 aagcggcaat cccttcaggg aattctttac gcgtgcgttt ttcaattttc tcggcgtctt
     2641 tcgggttcat taaacccgtg gtgcgtttgg caatcagtgc gtttaaatca atgccatcaa
     2701 tcaaatcaat cgggtcaagc acattgccct ttgacttaga cattttctga ccttctgcat
     2761 cacgaatcag gccatgcaca tacacgtgct taaagggaat tttgccggtg atgtgcgtgg
     2821 tcatcatcac catgcgcgcc acccagaaaa agatgatgtc aaaacctgta actaatacgc
     2881 ttgaaggcaa atattgttgt agcgcctgat tttgcgcgtc tgtcccatcg tcacctgtcc
     2941 aatccagtgt tgaaaacggc caaagggcac ttgaatacca agtgtccagc acatcgttat
     3001 cgcgcactaa attacctgtg tagccatctt tagcggctaa cactttagcg cctgcctcat
     3061 ccttcgccac atagacttta ccgctatctg aataccaagc gggaatttga tgcccccacc
     3121 ataattggcg agagatgcac caatcttgaa tattattgag ccattggttg taagtgttta
     3181 cccagttttc tgggtaaaac ttaatttcgc cgcttgctac aacatcaagc gctttttgcg
     3241 tgatcgattt gccatcagcc gcaggtttac tcatggccac aaaccattgg tcggtaagca
     3301 ttggctcaat aatcacacct gtgcggtcgc cacgcggtac tttaagtttg tgtttttcgg
     3361 ttttaactaa ataaccgttt tcttctaaat cagccaccac gagcttacgc gcagcaatgg
     3421 tggttaaacc acgatatttt tctggcgcat tttcgttgac taagcctgat aatgacaaca
     3481 tctctatcat cggcaatttg tggcgctggc ccacggcata gtcattaaag tcatgcgctg
     3541 gcgtgacttt aaccacgcct gtgccaaagg ctttatccac ataatcatcg gcaataatcg
     3601 gaatttcgcg gtcgcacaat ggtagtttta cgtttttacc aattaagtgt gcgtagcgtt
     3661 catcttcagg gtgcaccatt accgccacgt cacccagcat ggtttctggg cgtgtggttg
     3721 caaccgttag gtgaccttca ccactgcaaa gcggatagtt gatgtgccac ataaagccat
     3781 cttcttcttc ctgcaccact tccaaatctg atacagccgt gccgagtttc acatcccagt
     3841 tcaccaagcg tttaccgcga taaattagcc cttcgttata caaacgcaca aaggtctcgg
     3901 tgaccacttt attaagacca gcgtccatgg taaagcgctc gcgtgaccaa tcgggcgaag
     3961 tgcctaagcg gcgcatttgt tgcgtaatgg tgccgcctga ataggctttc cactcccaca
     4021 ctttttctaa gaatttttca cgccctaaat cgtgacgact cacgccttgc gcatctagct
     4081 ggcgctccac cacgatttgc gtggcgatgc cagcgtggtc tgtacccggt tgccacaaag
     4141 tattatcgcc acgcatacgg tgataacgcg tcagtgcatc catcaaggtt tggttaaacc
     4201 catgccccat gtgcaaagtg cctgtaacgt taggcggtgg caataaaatg cagaagttat
     4261 ctttcacttc tgggtttagg cctgcggcgt agtaaccttt gctctcccaa aatgcatacc
     4321 atttgctttc tatggttttg gggtcaaagg atttgtctaa cccttgggcg ctttcttgat
     4381 taggtggtgt tgtattttgc gtagtca
//
LOCUS       DAOQSI010000162         4375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_21390_length_4375_cov_4.816667, whole genome shotgun sequence.
ACCESSION   DAOQSI010000162 DAOQSI010000000
VERSION     DAOQSI010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21390_length_4375_cov_4.816667"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1837
                     /locus_tag="PKL42_09025"
     CDS             149..1837
                     /locus_tag="PKL42_09025"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit A"
                     /protein_id="HNU67256.1"
                     /translation="MITHLKNGMIYDPAHQKNGVKEDLYLFQGRIVPKPLDPGKITQT
                     FDLTGKVVMAGAIDMHSHIGGGKINIARMMLPEFQEKRLQETKGYTEPEAHVCTPNCS
                     HNATPSTTDTGFRYIEMGYTAAFEPAILPINARHAHLEMGDTPMIDKGGYAMLGNDDY
                     FLRLLHQNADQKAINDYVAWTLHATQAIGIKVVNPGGINAFKFNQRRLDLDENNVHYQ
                     VTPREILQKLSRAVHELGIAKPLHVHCNNLGAAGNFQTTLETMGASDGLPMHLTHIQF
                     HSYGTEGDKKFSSAAAQISEALNKNKHITADVGQILFGQTVTASGDTMMQHLNAKHAN
                     PKKSVIMDIECDAGCGVVPFKYRDQNYVNALQWAIGLELFLSIEDPWRIFLTTDHPNG
                     APFTSYPHLIRLLMDKTFRNEAFAKLNLDAQAMSNLTSLNREYSLYEIAIMTRAGAAK
                     LIGLNDCGHLGVGAQADITVYTDQPDREAMFAKPDYVFKNGQLVVKNGKVVKVVWGDT
                     HTAKPAFDMGVEKGLSEYFDKYHTIQLDNFKIADDEISDDGRGKIIANPSKSNQ"
     gene            1847..2506
                     /locus_tag="PKL42_09030"
     CDS             1847..2506
                     /locus_tag="PKL42_09030"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNU67257.1"
                     /translation="MSRLYGEQHRKLQDDFGTRAMADRVEELVCRTEFDEGAVGFIQS
                     SEMFFLSTVDHQGRPTVSYKGGDAGFVKVLDNTTLIFPSYDGNGMFFSIGNISTNPQV
                     GLLFISFETPNRLRVQGSASISNAPDLMAHYKEADFVVTVKLSEFWVNCPRYIPKMQK
                     VRDSRYVPRAHCETPLAGWKQLDLVQDVISNDDVEKAQKEGIITIEEWMGKVMTGDEK
                     A"
     gene            2510..3418
                     /gene="fhcD"
                     /locus_tag="PKL42_09035"
     CDS             2510..3418
                     /gene="fhcD"
                     /locus_tag="PKL42_09035"
                     /EC_number="2.3.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran--tetrahydromethanopterin
                     N-formyltransferase"
                     /protein_id="HNU67258.1"
                     /translation="MLINGIQIDDTFAEAFNMRGTRIIITAQNLKWAYHAANAMTGFA
                     TSVIGCGVEAGIERELSANETPDNRPGVSVLMFAMGSKVLMQQLETRMGQCILTCPTA
                     AAFAGITSEDNISLGKHLRYFGDGYQTSKLINGIRYWRIPVMDGEFLTQETTGMVRAI
                     GGGNFLILATSQPAALAAAETAVEAIKQVPNVIMPFPGGVVRSGSKVGSKYPKMFAST
                     NDAFCPTLKGLVQTELSDDIESVMEIVIDGLTFEDIAESMRVGIQAICNLGVASGVGR
                     ISAGNYGGKLGPHLFKLHDILSEKLS"
     gene            3415..4092
                     /locus_tag="PKL42_09040"
     CDS             3415..4092
                     /locus_tag="PKL42_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HNU67259.1"
                     /translation="MSALTFTLKKSPSAKLDCRQLTPNLLANLTIAKIKNIPLSSQKN
                     APKVADFFEVTGDDVQHIHFNNATAQLDYIGHHMTKGTIVVEGDCGDFLGANMQGGTI
                     IVKGSANDRLGDQMRRGLILIDGNAGDYCGSRMIAGTIGVYGTLGKHVGFAMKRGTIL
                     LTQTPKLHATMQNCCTHTLPFLALLFRAFTTLPTLFNTIQSHRVQRYAGDLACDGRGE
                     VLVFSSR"
     gene            4142..>4375
                     /locus_tag="PKL42_09045"
     CDS             4142..>4375
                     /locus_tag="PKL42_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67260.1"
                     /translation="MPQYLTAALYKFVSLPDYQSLQAPILAKCQQHHIKGTLLLAEEG
                     INGTIAGLAEDIHQVLDYLKNDALFKQRFAYLEH"
BASE COUNT         1233 a         1066 c         1007 g         1069 t
ORIGIN      
        1 tctttatacc agtcgccacg cccggcctag attgcaacgg cacactgttt agagtggatg
       61 gctcagtcac acttccgctc aaaaaatgtc gagaggctac ctaccccacg ctaagcgcca
      121 ttcttagcca aattgaggcg caactctgat gattactcac ctcaaaaacg ggatgattta
      181 cgacccagca catcaaaaaa atggcgtaaa ggaagacctt tacctgtttc aggggcgcat
      241 cgtgcctaag ccgttagacc caggtaaaat cacgcaaaca tttgacctca cgggcaaggt
      301 ggtgatggcg ggcgccattg acatgcatag ccatatcggc ggcggcaaaa ttaacattgc
      361 ccgcatgatg ttgcccgaat ttcaagaaaa aaggcttcaa gaaacaaaag gctatacaga
      421 gcctgaagca cacgtttgca cccccaactg cagccacaac gccacgccca gcaccactga
      481 tactggcttt cgctatattg aaatgggcta caccgcagcc tttgaacctg ccattttgcc
      541 tatcaatgct cgccatgcgc atcttgaaat gggcgacacg ccgatgattg ataaaggcgg
      601 ttacgccatg ttgggtaacg atgattattt cttgcgcttg cttcatcaaa acgcagacca
      661 aaaagccatt aatgattatg tcgcgtggac attgcacgcc acgcaagcga ttggcattaa
      721 agtggtgaac cctggcggta taaacgcgtt taaatttaac cagcgacgtc tcgatttaga
      781 tgaaaataat gtgcattatc aagtgactcc acgtgagatt ttgcaaaaac tcagccgcgc
      841 cgtgcatgaa ctcggcattg ccaaaccatt gcacgtgcat tgcaataatt taggcgcagc
      901 cggcaacttt caaaccacat tagaaacgat gggggcatcc gatggcttgc cgatgcacct
      961 gacgcatatc cagttccata gctacggcac tgagggcgat aaaaagtttt catcggccgc
     1021 tgcgcaaatt tccgaagctt taaacaaaaa caaacacatc accgctgatg tcgggcaaat
     1081 attatttggc caaaccgtaa ccgcatcggg cgacaccatg atgcagcacc tcaatgctaa
     1141 acacgccaac ccgaaaaagt cggtgattat ggatattgaa tgcgatgcag gttgtggcgt
     1201 ggtgccgttt aaatatcgcg accaaaacta cgtgaatgct ttgcagtggg caataggctt
     1261 ggagctattt ttgagcatag aagacccgtg gcgcatcttt ttaaccaccg accatccgaa
     1321 tggcgcgcca ttcactagct acccacattt aattcgttta ctgatggaca aaacctttag
     1381 aaacgaagcg tttgcaaaac tcaatttaga tgcgcaagcc atgagcaatc ttaccagctt
     1441 aaaccgcgaa tacagcctat atgaaattgc catcatgaca cgcgctggcg cggcgaagct
     1501 tattggcctc aatgactgcg gccatttagg cgtgggcgca caagccgata ttacggttta
     1561 tactgaccag cccgaccgtg aggccatgtt tgccaagccc gattatgtat ttaaaaatgg
     1621 tcaactagtc gtcaaaaacg gcaaggtcgt caaagtggtt tggggcgaca cacatacggc
     1681 caagcctgct tttgatatgg gtgttgaaaa aggattaagt gaatattttg acaaatacca
     1741 caccattcaa ctcgataact ttaaaattgc agatgatgaa atcagcgatg acggtcgtgg
     1801 aaaaatcatc gctaatccgt ctaaatcaaa tcaataagga taatctatga gcagacttta
     1861 cggtgaacag cacagaaaat tgcaagatga ctttggcacc agagccatgg ccgaccgtgt
     1921 ggaagagcta gtttgccgca cagagtttga tgaaggcgca gtcggtttta ttcaaagtag
     1981 cgagatgttt ttcttatcaa ccgtagacca tcaaggacgt ccaacggttt cgtacaaagg
     2041 cggcgatgcg ggttttgtaa aggtgctaga caacaccacg cttattttcc caagctatga
     2101 tggcaacggc atgttttttt caataggcaa tatcagcacc aacccgcaag ttggcttgct
     2161 gtttatttcg tttgaaacgc caaaccgtct acgcgtgcaa gggtctgcca gcatttcaaa
     2221 cgcccctgac ttgatggcgc attataaaga agcagatttt gtcgtcaccg taaaactctc
     2281 agagttttgg gtcaattgcc caagatatat tcctaaaatg caaaaagtgc gcgattcacg
     2341 ctacgtgcca cgcgcccatt gcgaaacgcc tctggcaggc tggaaacagc tcgacttggt
     2401 gcaagatgtc atttcaaatg atgatgttga aaaagcccaa aaagaaggca ttatcaccat
     2461 tgaagaatgg atgggcaaag tgatgacggg ggatgaaaaa gcctaattta tgttaataaa
     2521 cggcatacag attgatgata cttttgctga agcattcaat atgcgcggca cgcggattat
     2581 catcaccgcg caaaacctca aatgggctta tcatgcagct aacgccatga caggctttgc
     2641 caccagtgtg attggctgtg gcgttgaagc gggcattgag cgggaactgt ccgcgaacga
     2701 aacccctgac aatcggcccg gcgtaagcgt gctcatgttt gccatgggca gcaaagtatt
     2761 gatgcaacag ctagaaacac gtatggggca gtgcatcctc acctgcccta cagccgctgc
     2821 atttgcaggc attaccagtg aagacaacat cagcttaggc aagcacttac gctattttgg
     2881 agatggctat cagacttcca aactcatcaa cggcattcgt tactggcgca ttccagtgat
     2941 ggatggggag tttctcaccc aagaaaccac tggcatggtg cgcgccattg gtggcggtaa
     3001 ttttctcatt ttggccactt cgcagcccgc tgcgcttgca gccgctgaaa ccgctgttga
     3061 agccataaag caagtgccca atgtcatcat gccatttcct ggcggtgtgg tgcgtagcgg
     3121 ctcaaaggtt ggcagtaaat accctaaaat gttcgcatcc accaacgatg ccttttgccc
     3181 cacattaaaa ggcttagttc aaactgaact cagcgacgac attgaaagcg tgatggaaat
     3241 tgtgattgac gggctgacat ttgaggatat tgctgaatcc atgcgggttg gcatacaagc
     3301 tatttgtaat ttgggtgtag caagtggcgt agggcgcatt tctgccggca attacggcgg
     3361 taaattaggc ccgcatttat ttaagttgca tgatattttg agtgaaaaat tatcatgagc
     3421 gccctcacct ttactttaaa aaaatcgcca tccgccaaac tggattgccg acaactcacc
     3481 cccaatcttc tggcgaattt aaccattgct aaaattaaga acatcccgct ttcatcacaa
     3541 aaaaatgccc ctaaagtggc tgattttttt gaagttacag gtgacgatgt acaacatatc
     3601 cattttaaca atgccactgc acagctagac tatatcggcc accacatgac caaaggcacg
     3661 attgtggttg aaggtgattg cggtgatttt ctaggtgcaa acatgcaggg cggcaccatt
     3721 atcgtcaaag gtagcgccaa cgatcgccta ggtgaccaaa tgcgccgcgg cctcattctg
     3781 atagatggca acgccggcga ctactgtgga agccgcatga ttgctggcac cattggtgtt
     3841 tacggcacgc taggaaaaca tgtaggcttt gccatgaagc gcggcaccat tcttttaacc
     3901 caaacgccta aactgcatgc aaccatgcaa aactgctgca cacacaccct gcccttttta
     3961 gcattgctat ttcgcgcgtt cactacgctt ccaaccttgt ttaacaccat tcaatctcat
     4021 cgggtacaac gctacgctgg cgatttagct tgtgatgggc gtggcgaagt tctggtattt
     4081 tcatcaagat aattgcagcc gagtggtaaa atgtgctctt tgaacctaaa gtttgctttt
     4141 catgccccaa tacctcaccg ccgccttata taaattcgtc agcttgccag attatcaatc
     4201 tttgcaggcc ccgatattgg caaaatgcca gcaacatcac atcaaaggca cgctactact
     4261 ggctgaagaa ggtataaacg gcaccattgc cggtttggca gaagacattc atcaagtgct
     4321 tgattatctt aaaaatgatg cattatttaa gcaacgcttt gcttatctgg aacat
//
LOCUS       DAOQSI010000163         4356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_21548_length_4356_cov_5.034643, whole genome shotgun sequence.
ACCESSION   DAOQSI010000163 DAOQSI010000000
VERSION     DAOQSI010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21548_length_4356_cov_5.034643"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PKL42_09050"
     CDS             <1..711
                     /locus_tag="PKL42_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HNU67261.1"
                     /translation="DLEHKESYADEHPFYRMKVKLKKEIVTMGVPSVSPTKQVGTYVK
                     PEDWNALISDPDVVLLDTRNDYEVHIGTFKNAVDPKTTTFREFPEFVAKNLDITKNKK
                     VAMFCTGGIRCEKASSFMMAQGFEEVYHLQGGILKYLETLPEEESLWQGECFVFDQRV
                     GVKHGLAVGDYDQCYACRMPLSKEELQSPQYTAGISCPHCYDKISAEKRAALMERQKQ
                     VQLAKKRGETHIGEKQKK"
     gene            complement(851..1615)
                     /locus_tag="PKL42_09055"
     CDS             complement(851..1615)
                     /locus_tag="PKL42_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNU67262.1"
                     /translation="MEIDNILVEKIFSAIRSSYKIDKHVDFFVWLQGTVREVLPHDML
                     LACWGEFDEKQKRAALNYDVASNISNVRTQLIIDASKKIDVFMHHLHVLWVNNNQRWF
                     VRNHLNTQNEMDAIHAHFPSELAQFNSLLVYGVSDVRGNNECLYVFFSQQNTFNVQDS
                     VMGLVMPHIDSVLRKIQHLEPVEVIEALNMNAEAAVDLSGREHEILKWVRAGKTNQEI
                     GDILFISQNTVKSHLKRIFTKLNVTTRAQAVGKILN"
     gene            complement(1767..2318)
                     /locus_tag="PKL42_09060"
     CDS             complement(1767..2318)
                     /locus_tag="PKL42_09060"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HNU67263.1"
                     /translation="MLQTRNPQHLLAQSQIIHSTAAVEFSITRLSQEISTVLAEACPV
                     VICVMGGGIVFAGQLLTQLSFPLEIDYVHASRYANETSGSALVWKALPKSDLTGRTVL
                     LVDDILDEGITLLEIKQKCLALGAKEVYCAVLVEKKLNKIKPIQAHFVGLEVPNAYVF
                     GYGMDVYGWWRNLPAIYALQVSA"
     assembly_gap    2371..2470
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2514..3389)
                     /gene="galU"
                     /locus_tag="PKL42_09065"
     CDS             complement(2514..3389)
                     /gene="galU"
                     /locus_tag="PKL42_09065"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HNU67264.1"
                     /translation="MNKITKAVFPVAGLGTRFLPATKASPKEMLPIVDKPLIQYAAEE
                     AIAAGATELIFITGRNKRSIEDHFDNASELEASLEASGKKELLETLKAILPSHVSCIF
                     IRQPKALGLGHAVLCAKPVVGDHPFSVILADDLIDGTPAATKQMADVYARKQCSILGV
                     EDVLPSETASYGIVDAFEIEQNLLQLKSIVEKPKPADAPSTLAVVGRYVLTPKIFDCL
                     EKVKPGKGGEIQLTDGISDLMQFEDILAYRFAGTRYDCGSKLGFMKANVELALKHPEI
                     SKEFSEYLAQLLAKK"
     gene            complement(3405..>4356)
                     /locus_tag="PKL42_09070"
     CDS             complement(3405..>4356)
                     /locus_tag="PKL42_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HNU67265.1"
                     /translation="EQEFSRLKVPVKLLYMTGDVKNGRRMKELLDDFKPAVVFHAAAY
                     KHVPLMENGNVFEALSNNVLGTYTLAKACREANVEKFVLISTDKAVNPTNVMGASKRL
                     AEMVCQGLQSNPLSDRETSAKFVIVRFGNVLGSSGSVIPKFREQIAKGGPVTITHPEI
                     TRYFMSIPEAAQLVMQAGVMGQGGEIFVLDMGESVKIADLAKDMIKLSGLQLEDIKIE
                     YIGLRPGEKLYEELLADDENTLPTPHEKLRVASARNVNEDWVHNLLRWIETLQSADEE
                     YIKTELKLWVEEYSPQLKASAKHKAKAEAVAEIPKQITLH"
BASE COUNT         1233 a         1019 c          808 g         1196 t
ORIGIN      
        1 gacctggagc ataaagaatc atacgccgat gaacacccgt tttaccgtat gaaggtaaaa
       61 ctcaaaaaag aaatcgtcac tatgggtgtg cccagcgtta gccctaccaa gcaagttggc
      121 acctatgtaa agcccgaaga ctggaacgca cttatcagtg acccagacgt tgtcttactg
      181 gatacgcgca atgattacga agtgcacata ggcacattca aaaacgcggt tgaccccaaa
      241 accaccactt ttcgagagtt tcctgaattt gtcgccaaaa atctcgatat aaccaaaaac
      301 aaaaaagtcg caatgttttg tacgggtggc atacgctgtg aaaaagcctc ttcttttatg
      361 atggcgcaag gctttgaaga ggtctaccac ctgcaaggcg gcattttaaa atacctagaa
      421 accttgccag aagaagaaag tttatggcaa ggcgagtgct ttgtgtttga ccagcgcgtg
      481 ggcgtaaagc atggcctggc agttggcgat tacgaccaat gctatgcctg ccgtatgcca
      541 ctttcaaaag aagagttgca gagcccacaa tatacggctg gcatctcatg cccgcactgc
      601 tatgacaaaa tttcagccga aaaacgtgcc gcgctcatgg agcgacaaaa gcaggtacag
      661 ttagcgaaaa agcgtggcga aacccacatt ggcgaaaagc agaaaaaatg aatcaaattg
      721 gctaaattat gcgccaacct ttttcgatgc tctgtgagcc ttataaataa aggctcgcag
      781 agcataaaaa ttttctcggg taattttaat gtgattttta ggtttaaaat aacgcctcaa
      841 aacacttgtt ttaatttaaa attttaccca ccgcctgcgc gcgggtggtg acattcagct
      901 ttgtaaaaat gcgctttaaa tgacttttaa ccgtgttttg gctgataaat aaaatatcac
      961 caatctcttg attggtttta cccgcgcgca cccacttaag aatttcatgc tcgcgaccgc
     1021 ttaaatctac cgcggcctct gcattcatat tgagcgcttc aatcacttca actggctcaa
     1081 gatgctgaat ttttcgaagt acgctatcaa tgtgcggcat gaccaagccc atcacagaat
     1141 cttgcacatt gaaggtgttt tgctgactaa aaaacacgta taggcattcg ttgttaccgc
     1201 gcacatcact cacgccatac acgagcaatg agttaaattg tgcgagttct gaaggaaagt
     1261 gcgcgtgaat tgcatccatc tcattttgcg tattgaggtg atttctcacg aaccagcgct
     1321 ggttattatt cacccaaagt acatgcaaat gatgcataaa aacatcaatt tttttggatg
     1381 cgtcaataat cagttgcgtg cgcacattgc tgatattgga ggcgacatcg taattgagtg
     1441 ccgcccgctt ctgcttttca tcaaactcac cccagcaggc caacaacatg tcgtgcggca
     1501 aaacctctct tactgtacct tgcagccata caaaaaaatc cacgtgctta tcaatcttgt
     1561 atgaagaccg aatcgccgaa aatatttttt ctaccagtat gttgtcaatt tccatgagtt
     1621 tgttgttatt ttaagttaaa ttcgatttta cgcatcatta caaaaataat ctatatttaa
     1681 gtcaatttaa caaaaataaa caattgaata tcaataagtt atattgatag ccgtgaatac
     1741 agttaatcat ttaaggaaga aaatttctag gcactcactt gcaacgcgta aattgctggc
     1801 aagttccgcc accagccata aacatccatg ccataaccaa aaacatacgc attcggcact
     1861 tctaacccaa caaaatgcgc ctgaatcggc ttaattttat tcagtttttt ttctaccagc
     1921 actgcgcagt aaacttcttt tgcacccaat gccaaacatt tttgcttaat ttccaaaagc
     1981 gtgatgcctt catccagaat atcatccacc aataatactg tacggccagt taaatcagac
     2041 tttggcaatg ccttccaaac tagtgcacta ccgctggttt catttgcata gcggcttgca
     2101 tgtacgtaat caatctctaa tggaaaacta agttgcgtga gtaattggcc cgcaaacaca
     2161 atgccgccac ccatcacaca aatcactact gggcaagctt ctgcaagcac ggtagaaatt
     2221 tcctgactta aacgcgtaat ggaaaactca accgcagcgg ttgagtgaat gatttgcgac
     2281 tgcgctaaaa ggtgttgagg atttcgagtt tgcaacatcg tgtttcaatc ccattctcat
     2341 attgacttga agtgtacttg agaatttgca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn gagccttata aataaaggct cgcagcgatg cgcaaaaggt tagctacttt
     2521 ttagcgagca attgcgctaa gtattctgaa aactctttgc taatttcagg atgctttaaa
     2581 gctaattcta cgttggcttt catgaatcct aatttgctac cacagtcata acgagtgccg
     2641 gcaaaacggt aggccagaat atcttcaaac tgcatcaaat ctgaaatacc atcggtgagc
     2701 tgaatttcac cacctttgcc tggtttaact ttttcaagac aatcaaagat tttcggcgtc
     2761 agcacataac ggccgaccac cgccaaagtg gatggcgcat cggccggttt tggtttttca
     2821 acaatgcttt taagttgcaa taaattttgc tcaatttcaa aggcgtccac aatgccatag
     2881 cttgccgttt cagacggcaa cacgtcttca acacctaaaa ttgaacattg cttacgtgca
     2941 tacacgtcag ccatctgttt tgtggcagct ggcgttccat caattaaatc atctgccaaa
     3001 atcaccgaaa atggatggtc tcccaccact ggttttgcac acaataccgc atggcccaga
     3061 cctaacgcct taggttgacg aatgaaaata caagaaacat gtgaaggtaa aatagctttt
     3121 agcgtctcaa gcagttcttt ttttccactg gcttcaagtg aggcctctaa ttcactggca
     3181 ttatcaaagt ggtcttcaat cgagcgttta ttacggcctg taataaatat cagctctgtc
     3241 gcccctgccg cgatagcttc ttcagccgcg tattgaatca gcggtttatc aacaatcggc
     3301 aacatctctt ttgggctggc ctttgtagct ggcaaaaaac gtgtgcccaa cccagcaact
     3361 gggaaaaccg ccttggtaat tttattcatt tatggtttaa ctctctaatg caatgtaatt
     3421 tgttttggta tttcagcaac cgcctcagct tttgctttgt gcttggcact cgcttttaac
     3481 tgtgggctat actcttcaac ccataatttt aactcagttt taatgtattc ttcatctgca
     3541 gattgtaaag tttcaatcca gcgcaacaga ttatgtaccc aatcttcatt gacatttctt
     3601 gccgatgcca cacgtaactt ttcatgcggt gttggcagcg tgttttcatc atccgccagc
     3661 aattcttcgt acaacttttc acctggccgt aaaccaatat actcaatttt aatatcttcc
     3721 aattgcaggc ctgaaagttt aatcatatct ttggccaaat cagcaatctt caccgattcg
     3781 cccatatcga gcacgaaaat ctcaccgcct tggcccatca cacccgcttg catgaccaac
     3841 tgcgcggcct caggaattga cataaaataa cgcgtaatct ctggatgagt aatggtcacg
     3901 gggccgcctt tagcaatctg ctcgcgaaac tttggtatta cgctaccact gctccccagc
     3961 acattgccaa agcgcacaat cacaaactta gctgacgttt ctctgtcact cagcggatta
     4021 gactgcaaac cctgacacac catctctgct aaccgtttgc ttgcccccat cacattggta
     4081 gggttaaccg ctttatcggt agaaattaaa acaaatttct ccacattggc ttccctacaa
     4141 gcttttgcta aggtataagt gccaagcaca ttatttgata aggcttcaaa tacattgcca
     4201 ttttccatca acggtacatg tttataagca gcagcatgaa aaacaacagc gggtttaaag
     4261 tcatcaagca attccttcat tcggcgacca ttcttgacat ccccggtcat ataaagcaat
     4321 tttacaggca ccttaagccg gctgaactct tgttct
//
LOCUS       DAOQSI010000164         4281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_22130_length_4281_cov_4.007336, whole genome shotgun sequence.
ACCESSION   DAOQSI010000164 DAOQSI010000000
VERSION     DAOQSI010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22130_length_4281_cov_4.007336"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PKL42_09075"
     CDS             <1..519
                     /locus_tag="PKL42_09075"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897940.1"
                     /note="FabB, beta-Ketoacyl-ACP synthase I, KASI; catalyzes
                     a condensation reaction in fatty acid biosynthesis:
                     addition of an acyl acceptor of two carbons from
                     malonyl-ACP; required for the elongation of short-chain
                     unsaturated acyl-ACP; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HNU67266.1"
                     /translation="EEYEHAKARGAKIYAEVVGYGATSDGYDMVAPSGEGAVRCMQLA
                     LQGHDGKAIEKVDYINTHGTSTPVGDTKELEAIREVFGANNPVAIASTKSLSGHALGA
                     AGVNEAIYTLLMMENNFIAASANIETLDPAAEGLNIVRKTIENVKIETALSNSFGFGG
                     TNATLTLSTKNL"
     gene            567..1109
                     /locus_tag="PKL42_09080"
     CDS             567..1109
                     /locus_tag="PKL42_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1439 domain-containing protein"
                     /protein_id="HNU67267.1"
                     /translation="MKLKFLAMALILLSLVGCAGLGDRTVNVTEADVQNMLNKKLAVP
                     IELLKIFDVNLSNSIVKFDQTTGRMQTTLDTVLNSQMLGKSMTGKLGISGKLRFDAAT
                     QSIVLDDPSVDMFNIDGADGKYNEILNSLAKTVGGQMLNGMALYTVKPEDLKFGGATY
                     APKDMLVTDKGLQITLSPAK"
     gene            complement(1129..1497)
                     /locus_tag="PKL42_09085"
     CDS             complement(1129..1497)
                     /locus_tag="PKL42_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HNU67268.1"
                     /translation="MKLHLTSAENNYLITACGTQFVEVNKQQFTSSLLVMPQQLINDW
                     GVANFAELNEAHLEKIATFNPEVVLLGTGEKHQFLNPNITKSLMVKGIPLECMTTAAA
                     CRTYNILMSEGRNVLAALIV"
     gene            1640..2887
                     /locus_tag="PKL42_09090"
     CDS             1640..2887
                     /locus_tag="PKL42_09090"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNU67269.1"
                     /translation="MQPIKKSNKLANVCYDIRGPVLQRARQMEEEGQRIIKLNIGNPG
                     VFGLDVPEEMMQDVMRNMSKAGVYTDSKGLFEPRKSIMHYTQSKHIAGVTVDDIIIGN
                     GVSELIVMAMQGLLNNGDQVLVPMPDYPLWTAAVTLAGGTARHYLCDEATGWLPDLKD
                     MEAKITANTRGIVVINPNNPTGALYPRETLEGVIEIARRHKLVIFADEIYDKVLFDGN
                     EHTSIASLADDVLFVTFNGLSKNYRACGYRAGWMILSGEKKHASDFIEGLNMLASMRL
                     CANVPGQLAIQTALGGYQSIEDLVAPEGRLCKQRDIAYELLIAIPGVTCVKPKAAMYL
                     FPKLDPKIYPIKDDQQFILDLLLEEKVLLVQGTGFNWPTPDHFRVVFLPNVDDLTEAI
                     GRIARFLENYRKKHNSKLSGVKV"
     gene            2884..4197
                     /locus_tag="PKL42_09095"
     CDS             2884..4197
                     /locus_tag="PKL42_09095"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNU67270.1"
                     /translation="MKQLNVGLLGIGTVGGGTYTVLTRNAAEITRRTGVAINVVHVAD
                     KNVDHAKALTDGKVDVTNDAFAVVNNPNVDVVVELIGGYTLSKDLVLKAIANGKHVVT
                     ANKALLALHGNEIFAAAQKAGVIVAFEAAVAGGIPIIKALREGLGANKIEWVAGIING
                     TTNFILTEMREKGLTYQDVIGEAQRLGYAEADPTFDVEGIDAAHKLTILSAIAFGIPM
                     KFEEAYTEGITKLQQADIKYAEELGYRVKLLGITKRTAKGVELRVHPTLIPEKRLVAN
                     VNGAMNAVVVKGDAVGPTLYYGAGAGAEPTASAVVADLMDVARLSNASAEQRVPYLAF
                     QPNSVVDLPILPIAEVESAYYLRLRASDKPGVLAGVTKILADRNISIDAMLQKEPDDN
                     ETEADIVILTHVTVEKNMDAGISEIEALPATVGKVTRIRMEELGK"
     gene            4198..>4281
                     /locus_tag="PKL42_09100"
     CDS             4198..>4281
                     /locus_tag="PKL42_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67271.1"
                     /translation="MKYISTRGQSPALQFSEILLGGLAPDGG"
BASE COUNT         1199 a          840 c         1095 g         1147 t
ORIGIN      
        1 gaagaatacg aacatgccaa agcgcgtggt gcgaaaatct acgcagaagt ggtgggttat
       61 ggcgcaacat cagatggcta cgatatggtt gccccaagtg gcgaaggtgc agtgcgctgc
      121 atgcaattag cgttgcaagg ccatgatggc aaggcgattg aaaaggtcga ttacatcaac
      181 acgcacggca ccagcacacc agtgggcgac accaaagagt tggaggcgat acgcgaggtt
      241 ttcggcgcca ataatcccgt ggccattgct tcaaccaaat cactttctgg ccacgcgtta
      301 ggcgcggcgg gcgtcaatga ggcaatttac acgttgctga tgatggaaaa taactttatt
      361 gccgcttctg ccaatattga aacgctagac cctgcggcag aaggcttgaa tattgtgcgt
      421 aaaacaatag aaaacgtgaa gattgaaact gcgttatcga acagttttgg gtttggtggc
      481 accaatgcaa cattaacgct gtcaacaaaa aatctctaaa gacgttacat tttcaagaga
      541 ttttttaact aagtagaaag cctgtgatga agcttaaatt tttagcgatg gcactgattt
      601 tattgtcact agttggctgt gctggtttgg gcgaccgtac cgttaatgtg actgaagccg
      661 atgttcagaa catgctcaac aagaaattgg ccgtgccgat tgaactgcta aagatttttg
      721 atgtgaactt aagtaattcg atagtgaaat ttgaccaaac cactggccgc atgcaaacca
      781 cgctggatac ggtgcttaac agccagatgt tgggaaaaag catgactggc aagttaggta
      841 tttctggaaa attgcgtttt gatgcagcca cgcaatcgat agtgttggat gatccgtcag
      901 tggatatgtt taatattgat ggcgcagatg gcaaatataa cgaaatttta aattctcttg
      961 ccaaaaccgt gggcgggcaa atgctcaatg gcatggcgct ctacacagtt aaaccagaag
     1021 acttaaaatt tggcggtgcg acttatgcac caaaagatat gttagtaacg gataaaggtt
     1081 tgcagattac gctttcacct gcaaagtaat cgcacagatt taatggtttt atacaatcaa
     1141 ggccgcaagc acattgcggc cttcactcat taaaatgttg taagttctgc atgccgctgc
     1201 cgtagtcatg cactctagcg gaatgccttt taccataagt gattttgtta tgttagggtt
     1261 cagaaactgg tgtttttcgc ctgtgccgag caaaaccact tcagggttaa atgtagcaat
     1321 tttttcaaga tgtgcttcat ttaattctgc aaaattagct accccccaat cgttgattaa
     1381 ctgttgcggc atcacaagta agctgctggt aaattgctgt ttattcactt caacaaattg
     1441 cgtgccacaa gcggtgatga ggtaattatt ttctgcgctg gttaaatgta gtttcatgcc
     1501 gcaattttaa cagctttatg tgaatagtta agctgacggt ttcattgaaa caaggcccgg
     1561 ttctaaggta aactattggc tttcagtttt gtgtctttaa agcatgctta tggcgtgcat
     1621 ttttagaaaa atcctcatga tgcagccaat taaaaagtca aataaacttg ccaacgtttg
     1681 ctacgatatc cgtggcccag tgttgcagcg cgcccgtcaa atggaagaag agggccagcg
     1741 cattatcaag ctcaatattg gtaacccagg tgtgtttggt ctggatgtgc ctgaagagat
     1801 gatgcaggat gtgatgcgca acatgagcaa agcgggcgtg tacacagaca gcaaaggcct
     1861 atttgaacca cgcaaatcta ttatgcatta cacacaaagc aagcatattg cgggtgtgac
     1921 ggtagatgac attattattg gcaatggcgt ttctgagctg attgtgatgg cgatgcaggg
     1981 tttgctcaac aatggtgacc aagtgttggt gcccatgcca gattatccat tgtggacagc
     2041 tgccgtgacc ttggctggtg gtacagcacg acattatttg tgtgatgagg ctacaggctg
     2101 gttgcccgac ttaaaagata tggaagccaa aatcaccgcc aatacccgtg gtattgtggt
     2161 gattaacccc aacaacccaa ctggtgcttt gtatccgcgt gagaccttgg aaggtgttat
     2221 tgagattgcg cgccgccata aattggtgat ttttgcggat gaaatttatg acaaagtgct
     2281 gtttgatggc aatgaacata cttcaattgc atcccttgcc gatgacgtgt tatttgtaac
     2341 gtttaatggc ttatccaaaa attatcgtgc gtgtggctac cgcgctggct ggatgatttt
     2401 aagtggtgag aaaaagcacg ccagtgactt tattgaaggc ttgaatatgc tggcctcaat
     2461 gcgtttatgt gccaatgtgc ctgggcagtt ggctattcaa actgcgctgg ggggctacca
     2521 aagtattgag gatttagtgg caccagaagg ccgtttgtgt aaacagagag atatcgctta
     2581 tgagttgctg attgcaattc cgggcgtaac ctgtgtcaaa ccaaaagcgg cgatgtacct
     2641 atttccaaaa ttagacccaa aaatttaccc aatcaaagac gaccaacaat ttattctaga
     2701 tttgttgctg gaagaaaaag tattgctcgt gcagggtact ggctttaact ggccaacacc
     2761 tgaccatttc cgtgtggtgt ttttaccaaa cgtggatgac ttgaccgaag cgattggccg
     2821 aattgcacgg tttttagaaa attatcgaaa aaagcataat tcaaagttaa gtggagtgaa
     2881 agtttgaaac aattgaatgt aggtttgctg ggcatcggca cagttggtgg tggtacatat
     2941 accgtattaa cccgtaacgc ggctgaaatt acacgtcgta caggcgttgc gattaacgtt
     3001 gtgcatgtgg ctgataaaaa cgtggaccat gcaaaagcgc ttacagacgg taaagtagac
     3061 gtgaccaatg atgcatttgc agtggtcaac aatcccaatg tggatgtggt ggtggaactc
     3121 attggtggct ataccttaag caaagacttg gtgttaaagg ccattgccaa tggcaaacat
     3181 gtggtgactg caaataaagc cttactcgcc ctgcatggca acgagatttt tgcggccgcg
     3241 caaaaagcgg gtgtgattgt ggcatttgaa gccgcagttg caggcggcat cccgattatc
     3301 aaggcgttac gcgagggttt gggtgcaaat aaaattgagt gggtggctgg tattatcaat
     3361 ggaactacca actttattct caccgaaatg cgtgaaaaag gtctgaccta tcaagatgtg
     3421 attggcgagg cacaacgttt aggttatgcc gaagctgacc ctacctttga tgtggaaggc
     3481 attgatgcgg cgcataagct cactatattg tcagccattg cgtttggtat accgatgaag
     3541 tttgaagaag cctacactga aggcattacc aagcttcagc aagcggatat taaatatgct
     3601 gaagaacttg gctaccgcgt aaagttactt ggtattacta aacgcactgc caaaggtgtt
     3661 gaactgcgtg tgcatccaac gcttatccct gaaaaacggt tggttgcaaa tgtgaacggt
     3721 gcaatgaacg cagtggtagt gaaaggcgat gccgttgggc caacgcttta ttatggcgcg
     3781 ggcgcagggg ctgaaccaac ggccagtgcg gtggtagctg atttaatgga cgtagcacgc
     3841 ttgagcaatg ccagcgcaga acagcgtgtg ccatatttag catttcagcc caatagcgtg
     3901 gtggatttac caattttacc gattgcggaa gttgaaagtg cttactacct gcgtttacgt
     3961 gcctcagata aacctggcgt gttagcgggc gtgactaaaa tattagcaga ccgcaatatt
     4021 tcgattgatg ctatgttgca aaaagaaccc gacgacaatg aaactgaagc tgatattgtg
     4081 attttgacgc atgtgacggt tgaaaaaaac atggatgcgg gtatttctga aattgaagca
     4141 ctgccagcca cagttggtaa agtcacacgt attcgtatgg aagaattagg gaagtaaatg
     4201 aaatatatca gcacgcgcgg gcaatcgcct gcattacagt ttagtgagat tttattgggt
     4261 gggctagcgc cagatggcgg t
//
LOCUS       DAOQSI010000165         4275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_22173_length_4275_cov_4.515877, whole genome shotgun sequence.
ACCESSION   DAOQSI010000165 DAOQSI010000000
VERSION     DAOQSI010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4275
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22173_length_4275_cov_4.515877"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1186)
                     /locus_tag="PKL42_09105"
     CDS             complement(<1..1186)
                     /locus_tag="PKL42_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNU67272.1"
                     /translation="MNNTIKLAVASALLAAASSANAGISIPAGDWTLDIGGVVNAYYT
                     HTSFSGDTGAGAAPLGLGDGDQDSSNNITTGLLPNYLSVSGKTRQNDLDVGFTISINP
                     GASTTTGGLQSAHQENRQAFLTFGDASWGSMKLGKDLGIYASDAILNDMTLLGVGSGA
                     GNLAGNTTTLGRIGTGFMYADWKSQVAYSSPNWNGFSFTVGLTQGWNAVDTLGTSASS
                     ANRSGSQSAYEGKASYEWAGDFGGKVWASAITQKVDTLTAGGDDRATAWDIGATVNVA
                     GFGLTGYYGDGDGIGQTIQLFDGFDANGKSRDSDQWYVQATYTLPGVGTKLGASYGQS
                     TLDGNSVDGFRDIEDNMWTVGAYHPLTKHLNLVAEYSESERDVDNRGAGNAKADAKTI
                     ALG"
     gene            complement(1519..2643)
                     /locus_tag="PKL42_09110"
     CDS             complement(1519..2643)
                     /locus_tag="PKL42_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNU67273.1"
                     /translation="MNTLAPYAANPAQTKGRQFDEPASAPRNAFQRDRDRIIHSTAFR
                     RLEYKTQVFVNHEGDLFRTRLTHSLEVAQLARGIARTLNLHEDLTEAIALAHDLGHTP
                     FGHAGQDALNTCMKNHGGFEHNLQSLRVVEKLEQRYAEFDGLNLTFETREGILKHCSI
                     KNAKQLGDVGQRFLDKTSPSLEAQLTNFADEIAYNNHDVDDGLRSGLISIEQLSQVEL
                     FANNQQMVAKQYPNLEQKRLIPETIRRMINTLVTDLCTQSAKNIAQHQPQSIADIRKL
                     PPLIRFSAEIAAQNHKLKQFLRKNLYHHYQVNRMSAKATRIVQELFSCFYENTGLLPD
                     EYQAFAKTDKARAVADYIAGMTDRFAIREHRRLFAVEEHW"
     gene            complement(2708..3781)
                     /gene="aroB"
                     /locus_tag="PKL42_09115"
     CDS             complement(2708..3781)
                     /gene="aroB"
                     /locus_tag="PKL42_09115"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNU67274.1"
                     /translation="MQTLNVELASRSYPIHISRNLLSQAELILPYLKRKQVAIVTNTT
                     VAPLYLDKLSKTLINAGVRVTPIILPDGEAYKNSETLNKIYDALLESRSERSTTLIAL
                     GGGVIGDLTGYAAATYLRGVPFIQIPTTLLSQVDSSVGGKTGINHPLGKNMIGAFYQP
                     QLVLADIDTLQTLPPREFSAGVAEVIKYGLIRDADFFDWLEVNMEKLMALDEAVLSYA
                     IFRSCQNKAEVVARDEHENGERALLNLGHTFGHAIENAMGYGVWLHGEAVAAGTMMAA
                     RLSERMGWLTNQDVQRIHAIFTAANLPLNAPKLGVEKYLNLMQLDKKVADGKIRLVLQ
                     QGIGKSVITSDYDIEKLRETLAL"
     gene            complement(3796..4080)
                     /locus_tag="PKL42_09120"
                     /pseudo
     CDS             complement(3796..4080)
                     /locus_tag="PKL42_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149227.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
     gene            complement(4041..4223)
                     /locus_tag="PKL42_09125"
     CDS             complement(4041..4223)
                     /locus_tag="PKL42_09125"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67275.1"
                     /translation="MAAAAASMLAASTVSGFSTVGWATAYFKEVDATDVLLVLGKATL
                     SGYLVALCCYLRTVLF"
BASE COUNT         1132 a          980 c          892 g         1271 t
ORIGIN      
        1 cgcctaaagc gattgtttta gcatctgctt tagcattacc tgcaccacgg ttgtctacat
       61 cacgctctga ttctgagtac tcagcaacca agttcaaatg ttttgtcaat gggtgataag
      121 caccaactgt ccacatgttg tcttcgatat cacggaaacc atcaacactg ttaccatcta
      181 aagtagactg accgtatgaa gcgcccaatt tagtacctac gcctggcaat gtgtaagttg
      241 cttgcacgta ccattggtct gaatcacgtg atttaccatt agcgtcaaaa ccatcaaaca
      301 attggattgt ttggccgata ccgtcgccgt caccgtagta acctgttaag ccaaaaccag
      361 ccacgtttac agtagcacca atgtcccatg cagttgcacg gtcatcgcca ccagcagtta
      421 aagtatcaac tttttgcgta atggctgaag cccaaacttt accaccgaag tcgccagccc
      481 actcgtatga agctttacct tcgtaagcag attgtgaacc tgaacggtta gcactagaag
      541 cacttgtacc taatgtatca actgcattcc aaccttgtgt taaaccaacg gtgaagctga
      601 aaccattcca gtttggtgat gagtaagcca cttgtgattt ccagtcagca tacataaaac
      661 cagtaccgat acggcctaat gttgttgtgt taccagctaa gttaccagca cctgaaccaa
      721 cacctaacaa tgtcatgtcg ttcaagatag catcagaagc gtagatacct aaatctttac
      781 caagcttcat tgaaccccat gaagcatcac cgaatgttaa gaatgcttga cggttttctt
      841 gatgagctga ttgaagacca ccagttgtag tagaagcgcc tgggttgatg ctgatggtga
      901 aaccaacatc taagtcgttt tgacgtgttt taccagaaac tgataaatag ttaggtagca
      961 aacctgtagt gatgttgttt gatgaatctt gatcaccatc acctaaacct agtggagctg
     1021 caccagcacc agtatcgcca ctgaatgatg tgtgtgtgta gtatgcgttt acaacgccac
     1081 cgatgtctaa agtccaatcg ccagctggaa ttgagatacc agcgttagcg cttgaagctg
     1141 cagctaataa agctgaagca actgctaatt taattgtgtt attcatataa atctcctaaa
     1201 agtttattgc ttatcgtttt actacttaag ttacctcacc attcaccagt gattgctaac
     1261 tggcgtttag caagagagtt tgcaaactct ctcaatgctt tgctagtttc tattgcaaca
     1321 aagttctttt gttacaaaca gaatagtttc attaagaagg tgaatgagag tatgcctaaa
     1381 ggcggaattt gaaacaacaa gttatttgca ttcttgacac aaatatttac atttatgatt
     1441 ttttgttgta aatgtgcaac attatggtgc gttgtcgtaa tgttttcgta atcttgaagt
     1501 tcgcttctac aaacctttct accaatgttc ttcaaccgca aacaacctgc gatgctcacg
     1561 aatagcaaag cgatctgtca tgcctgcaat gtagtcggcc actgcgcgtg ctttgtcagt
     1621 ttttgcaaat gcttggtatt catctggcaa tagcccagta ttttcataaa agcagctaaa
     1681 tagctcttgc acaatgcgag tggcttttgc actcatgcgg ttaacttgat aatgatggta
     1741 taaatttttg cgtaaaaact gctttaattt gtgattttgc gctgcaattt ctgcgctaaa
     1801 ccttatcaac ggtggcaatt tacgaatatc tgcaatgctt tgcggctgat gttgcgcaat
     1861 gttttttgcg ctttgtgtgc ataaatcggt caccaatgtg ttaatcatgc ggcgtatggt
     1921 ttctggaatt agccgttttt gctctaaatt tgggtattgt tttgcaacca tctgctgatt
     1981 attggcaaac aattccactt ggctcagttg ttcaatactg attaagcctg agcgtaggcc
     2041 atcatctaca tcatggttgt tgtaggcgat ttcatcggca aagttggtga gttgcgcctc
     2101 caggcttggg cttgttttgt ctaaaaaacg ttggcccaca tcgcctaact gtttggcatt
     2161 tttaattgag caatgcttta gaataccttc tcttgtttca aaggtaaggt ttaacccatc
     2221 aaattcagca tagcgctgtt caagtttttc taccacgcgt agcgattgca ggttgtgctc
     2281 aaaaccaccg tggtttttca tacacgtgtt taatgcatct tgcccagcgt ggccaaaagg
     2341 tgtgtggcct aagtcatgcg cgagggcaat ggcttctgtt aggtcttcat gcaaatttag
     2401 ggtgcgtgca atgccgcgcg cgagctgggc aacttctaag ctgtgtgtta aacgggtgcg
     2461 aaataaatca ccttcgtgat ttacaaacac ttgcgtttta tattctaagc gccgaaaagc
     2521 ggttgagtga ataatgcggt cacgatcgcg ctgaaacgca ttgcgcggtg ctgatgctgg
     2581 ctcatcaaat tggcggcctt ttgtttgtgc tggattggcg gcgtaggggg cgagagtgtt
     2641 catgtattag atttctttta tgtgttacat tgttcgcggt cagtgaccgc aaacaatgtg
     2701 aaatttatca taacgccaat gtttctctta acttttcaat atcataatca ctggtaatca
     2761 ctgattttcc aataccctgc tgaagcacca gtctaatttt tccatccgct acttttttat
     2821 ctaactgcat taagttcaga tatttttcaa ctcccaattt tggtgcgttt aatggtaagt
     2881 ttgctgctgt gaatattgca tgaatacgtt gaacatcttg attagttagc cagcccatac
     2941 gttccgataa acgtgccgcc atcatcgtcc cagcggctac ggcttcacca tgcaaccaca
     3001 cgccataacc catggcattt tcgatggcgt gcccaaaagt atggcccaaa ttaagcaagg
     3061 ctctttcacc attttcatgt tcgtcacgtg cgacgacttc tgctttgttt tggcatgatc
     3121 taaaaatggc gtagctcaaa acggcctcat ctagcgccat gagcttttcc atattgactt
     3181 caagccaatc aaaaaaatcg gcatcccgaa ttagcccata cttaatcacc tccgccacgc
     3241 ctgccgaaaa ttcgcgtggc ggcaaggttt gcaaggtatc tatatctgcc agcactaatt
     3301 gaggctgata gaaagcaccg atcatgtttt tgcccagcgg atggttaatg ccagtttttc
     3361 cacccactga agaatctact tgtgaaagca acgttgttgg aatttgaata aagggtacgc
     3421 ctcttagata agtggcagcg gcataacccg ttaaatcacc aatcacaccg ccaccaagcg
     3481 caataagcgt tgtactgcgc tcggagcgac tttctaacaa tgcatcgtaa attttattga
     3541 gtgtttcact gtttttatag gcttcaccat caggcaatat aatcggggtt actcgaacgc
     3601 cagcattaat cagcgttttg cttaatttat ctaagtacag tggcgcaaca gtggtatttg
     3661 tgacgatagc cacctgcttg cgcttaaggt agggcaaaat taattccgct tgtgataata
     3721 aattacgact aatatgaatg gggtaacttc tactggcgag ttcaacatta agtgtttgca
     3781 tatttaaaat ttaatttatt ttggtttagg gttacttaat aactgctcta tataatgcgt
     3841 aatttcattt gcggattgcg ggccagtatt gaccacacaa tctgctaatg ccgtgtaaat
     3901 tgggtcacgc tccaaaaaaa gctgttctaa tttttgctta atattgccac cttgtaacaa
     3961 agggcgagat ttgtcatttc tggtacgcaa ccataaatca tgcacatttc cgcgcaaata
     4021 aattaccttg ccattacctt ttaaaaaagt acggttctca ggtaacagca cagcgccacc
     4081 aggtatcccg acagggtcgc tttgccgagc accagcagca catcggtggc atcgacctcc
     4141 ttgaagtagg ccgtcgccca gcccactgtc gagaatcccg acaccgtgct cgccgccagc
     4201 atcgacgcgg ccgccgccgc catgaccccc gccagcgtca ccaccggcat cgccagggtc
     4261 atgccccaga ccaac
//
LOCUS       DAOQSI010000166         4240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_22459_length_4240_cov_4.370370, whole genome shotgun sequence.
ACCESSION   DAOQSI010000166 DAOQSI010000000
VERSION     DAOQSI010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22459_length_4240_cov_4.370370"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="PKL42_09130"
     CDS             <1..1081
                     /locus_tag="PKL42_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNU67276.1"
                     /translation="IKPGKVYNQTDLLTFQTRLQEIGYFKNVEVTADTQLANHENNNA
                     PIKVVVEENKSIKVGVGAGYSTNTGARVQFTYDDLDFMDRDWRFASSLKVEQKAQFLG
                     GQIQFPVTSKGYRDSMSADMNRTAFEGQTVTSSQLGIKRTWGPRKREQYLGANYLIEQ
                     QDVDGGETTTKKVGTLNYGITLRRTDNDLAPMRGYLFNAQFAAAPLDSLSNGRFLHSY
                     IKTQAYYPITSSTQLIARAELGMVNGKNSAPETFLFRAGGDQSVRGYAFQSLGIKEAN
                     ATVGGRYLATGSVEVVQWLTQQWGAAVFVDAGNVANLWQDLKPVYGYGLGVRWKSPLG
                     PIGADIAYGQEVDDYRLHFNIGVAF"
     gene            1123..>4240
                     /locus_tag="PKL42_09135"
     CDS             1123..>4240
                     /locus_tag="PKL42_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNU67277.1"
                     /translation="MLAALFVIQTAHGEMSISVNSSLDSFSHDLEGMHLKLEKLDASW
                     QLSPFGDGQLTVDKIRAKRLTITFKTQPKNAGDNALPERIKLPFPVNIRQAEVAELLL
                     MSGDQSYQFNQVRFALEADTQTIHIKQLRADTPWGETNLKLDVNTLKPFALSGEVSLK
                     QSTDNVPYDVLAQFSGDLHHLNFASKAMLSKQAGKFMIYQQNQQQLAPAALVALNGQL
                     DLIGDYPLAITTNISQLNPERLGDYPAAMLNFNIDLQGTLLPETNVDVAFKSHDSVWQ
                     NQPFTSTGKFSLTAEKIRTFDLEAAISNNHIKANGILNEIASRIEWQANLTDISKLGQ
                     GYSGMLRADGVIEGMLKDLFAGEKNQFSLQFNLAGEKLSLPQGLKIAQLNGQASMKPE
                     ADGTVTGEFKANGLQYNQYPQLDSQITLAGTKRNHKININAVGKHMQFDSLLEGGISE
                     NQWQGLLQKLSYTGQHPVQLIKPANLKFGLSNLSADNVQLQLTKGRALIDQLKFNKQE
                     LTTTGRLENIALDDLPKERLHLPDTLQGEATFSGRWDVHAKENLNGRLKFWKESGDLT
                     LASHDGSQRPLGLETLALDIAITNNETVLSTQLKGRNIGNAELLFTTILTNTDAGYAL
                     RAEAPLSVKGTVQLHTLALLPLPTSLMDATLDGELDLSVNGQGTLRTPNLSGSVRAKN
                     LQFMLPSEGVALTQGLLDAQFENDQLTIQKASWQGGEGFVETNGFMRLDKGKPIINLA
                     WNAQEFTVISRTDRLLVMSGKGATELTDNILSITGNFTVNRGLAELANEDTPVLGDDV
                     IVLGQSEAIQERALQILLNGLRVSLGDDFRLRGRGLDAELAGALTFTGLTQYRPHTEG
                     SIQITKGTYMAYGQVLTIERGIFNFNGTVDNPGINVRAMRNSKPVNAGVEITGSAQIP
                     ATKLVSDPSVSDSEKLSWLVLGHGMDQTTKNDYGLLSLAAGAILSQGQSVPLQTQIAH
                     AAGLDELSFSGGDANSASLVFGKRLTSRLYLSYVKSISGLLDVARLTYNVTSRWSLRA
                     EAGTESAVD"
BASE COUNT         1243 a          958 c          971 g         1068 t
ORIGIN      
        1 cataaaacct ggaaaagttt ataaccagac cgatttattg acctttcaaa ctcgcctaca
       61 agaaattggc tactttaaaa atgtcgaagt cactgcggat acgcaattag ccaaccatga
      121 aaacaataat gcacccatta aagtggtagt agaagaaaat aaaagcatca aagtgggggt
      181 tggtgcgggc tacagcacca atacgggtgc gcgggtgcaa ttcacctatg atgacctaga
      241 ttttatggat cgtgactggc gctttgccag cagtttaaag gttgagcaaa aagcgcagtt
      301 tcttggcgga caaattcagt ttcctgtgac cagcaaaggt tatcgagaca gcatgagcgc
      361 cgacatgaac cgcaccgcat ttgagggaca aacagtaact tccagccaac tcggcattaa
      421 acgtacatgg ggtccacgta aacgagaaca atatttaggt gccaattatc tgattgaaca
      481 gcaggatgtg gatggtggcg agaccaccac taaaaaagtg ggtaccctta attatggcat
      541 caccctcagg cgcacagata acgatttagc acccatgcga ggttatttat ttaatgcgca
      601 atttgcagcc gccccgctag attcactatc aaatggccgc tttttacaca gctatattaa
      661 aacgcaagcc tattacccca ttaccagtag cactcagttg attgcccgcg ccgaactagg
      721 catggtgaat ggcaaaaata gcgcgcctga gacgtttttg ttccgcgctg gtggcgatca
      781 atcggtacgc ggttacgcat ttcaaagctt aggtatcaaa gaggccaatg caacggtcgg
      841 tggccgctac cttgctacag gcagtgttga agttgtgcaa tggcttacgc aacaatgggg
      901 ggcagctgtt tttgtagatg caggcaatgt tgcaaatcta tggcaagact taaaacctgt
      961 ttatggctat ggtttaggtg ttcgctggaa aagcccgctt ggaccaattg gggcagacat
     1021 cgcctatggg caagaagtgg atgactatcg actgcacttt aatattgggg tggcttttta
     1081 atgctacgcc gcacttacct ttgcatttat gctctatgcc tgatgctggc agcgctattc
     1141 gtcattcaaa cagcccatgg tgaaatgtcg attagcgtta attccagtct cgattcattt
     1201 agccatgatt tagaaggcat gcatttaaaa cttgaaaaac tagatgcttc atggcaactc
     1261 tcaccctttg gtgatggcca attgactgta gataaaatcc gtgcaaaaag actcactatt
     1321 acctttaaaa cccagccaaa aaacgctggt gataacgcgc ttcctgagcg cattaaactg
     1381 ccatttcctg tcaatattcg tcaagccgag gtggctgagt tgttacttat gagtggtgac
     1441 caatcttacc aattcaatca agtaaggttt gctttagaag cagatacgca aacgattcac
     1501 attaaacagt tgcgcgccga tacgccgtgg ggtgaaacca acctaaagct tgatgtaaat
     1561 acactgaagc catttgcgct ttccggtgag gtttcgctca aacaaagcac cgataatgta
     1621 ccttatgatg tgctggcgca attttctggc gacttgcacc accttaactt tgcaagcaaa
     1681 gcaatgctct caaaacaagc gggtaaattc atgatttacc aacaaaacca gcaacaactg
     1741 gccccagccg cgttggttgc attgaatggt caactagatt taattggaga ttaccccctt
     1801 gctattacca caaacattag ccaactgaac cccgaacgtt taggtgacta ccctgcagca
     1861 atgcttaatt ttaatatcga tttacaaggc acgcttttac ctgaaaccaa tgttgatgtt
     1921 gcgttcaaat cgcacgacag cgtttggcaa aatcagccat ttaccagcac aggcaaattc
     1981 agtttaactg ctgagaaaat acgcactttc gaccttgaag cagctatcag taacaaccac
     2041 attaaggcta atggtatcct gaatgaaata gccagccgta ttgagtggca agccaattta
     2101 acggatatca gcaagctcgg ccaaggctat tctggaatgc tgcgtgccga tggcgtgatt
     2161 gaaggtatgc ttaaagattt atttgcaggt gaaaaaaatc aattttcact ccagttcaat
     2221 ttagccggag aaaaactaag cctgccacaa ggcctgaaaa tagcgcaact caatggtcaa
     2281 gcatcaatga aacctgaagc agacgggact gtgacggggg aattcaaagc aaatggtctt
     2341 caatataatc aatacccgca gcttgatagc caaatcacct tagctggcac taaacgcaat
     2401 cacaaaatca acatcaacgc agtcggtaag cacatgcaat tcgactcgtt acttgaaggc
     2461 ggaatatctg agaatcaatg gcaaggtttg ttgcaaaaat taagctatac cggtcaacat
     2521 ccggttcagc tcataaaacc agctaacctc aaatttggac tgtcgaatct ttcggctgac
     2581 aacgtgcagt tacaactgac taaaggccgc gccttgattg accaattgaa attcaacaaa
     2641 caagaattga cgacaactgg ccgacttgaa aatatcgcac tagatgattt accaaaagaa
     2701 cgcctacatt tgcctgacac ccttcaggga gaagccacgt tttcgggaag gtgggatgta
     2761 cacgcaaaag aaaatttaaa tgggcggtta aaattttgga aagaatctgg cgatttgact
     2821 cttgccagtc atgatggctc acaaaggcca ctaggtttag aaactctagc acttgatatc
     2881 gccattacga ataacgaaac ggtgttgtca acacaactca aaggacgcaa tattggcaat
     2941 gctgaactcc tttttacaac aattttgact aacactgacg caggctatgc tttgcgtgcg
     3001 gaggcgcctt tgtcagtaaa aggcactgtg caattacata ctttggcttt gctaccactc
     3061 cctacgtctc ttatggatgc aacgctggat ggtgagcttg atttatcagt caatgggcaa
     3121 ggtacattgc gtacacctaa tcttagtggc agtgtacgag caaaaaatct gcaattcatg
     3181 ttaccttctg aaggtgttgc gctgacacaa ggcttgcttg acgcgcaatt tgaaaatgac
     3241 cagctcacca ttcaaaaagc ttcttggcaa ggcggcgaag gttttgttga aaccaatgga
     3301 tttatgcgtt tagataaagg caaacccatc atcaacctag catggaatgc acaagagttc
     3361 actgtaattt cacgtactga tcggttgtta gtcatgagcg gcaaaggcgc tactgaatta
     3421 actgacaaca tactcagtat tacaggcaat tttactgtga atcgcggttt agcagagctt
     3481 gccaatgaag acacgcccgt tttgggggat gatgtgattg tgttaggcca atcagaagcc
     3541 atacaggagc gggcattaca aatactactc aacggcttac gcgtcagttt gggagatgat
     3601 ttcagattgc gcggccgcgg gttggatgca gagctggctg gcgctcttac cttcaccggt
     3661 ttgacgcagt accgtccgca tacagaaggc agcattcaga ttacaaaagg cacttatatg
     3721 gcttatgggc aagtgctgac aatagaacga ggtatcttca attttaacgg caccgttgat
     3781 aacccaggca tcaatgttcg cgccatgcga aacagtaagc ccgtgaatgc cggtgttgag
     3841 attacaggta gtgcgcaaat tccagcaacc aaactggttt ccgacccgag cgtttcagat
     3901 agtgaaaaac tttcatggct agtcttaggc catggcatgg atcaaacaac aaaaaacgat
     3961 tacgggctgc tatcgcttgc tgcgggcgcc attttgtcgc aagggcagtc tgtgccgcta
     4021 caaacacaaa ttgcccatgc tgctgggctg gatgaactga gcttttcagg tggggatgcc
     4081 aacagcgcct cgctagtatt tggcaaacgt ctcacctcaa gactttactt aagttacgtt
     4141 aaaagtatta gcggcttact tgatgtcgct cggttgacct acaatgtcac ctcgcgctgg
     4201 tctctacgtg ccgaagcagg cactgaaagc gcggtagatg
//
LOCUS       DAOQSI010000167         4231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_22532_length_4231_cov_5.491140, whole genome shotgun sequence.
ACCESSION   DAOQSI010000167 DAOQSI010000000
VERSION     DAOQSI010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22532_length_4231_cov_5.491140"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /gene="mog"
                     /locus_tag="PKL42_09140"
     CDS             <1..545
                     /gene="mog"
                     /locus_tag="PKL42_09140"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HNU67278.1"
                     /translation="ASSGIYEDKGIPNLQSWLELAVKSPFSLEKRLISDEQSLIESTL
                     VDLVDNQACHLILTTGGTGPALRDVTPEATLAIADKTMPGFGEQMRQISLNFVPTAIL
                     SRQVAVVRKQCLIINLPGQPKAIAQTLQGLKDETGNTKVHGIFSAVPYCLDLLGTPEK
                     PMPYLETHEQIVKAFRPKSS"
     gene            639..1859
                     /locus_tag="PKL42_09145"
     CDS             639..1859
                     /locus_tag="PKL42_09145"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNU67279.1"
                     /translation="MLKTIPTHQVTLGMHIHALNGAWIDHPFWKSKFVLNDPEDLKKL
                     RASSIKEVVIDIAKGVDVKTSETATTAPAATQPAESVAEQPQTPIEKKAVARVSAAEE
                     QVRAKKILQESKKAVTTMLNDVRMGKAVNPGTATALVEEIASSVSRNESALISLVRLK
                     TKDDYTYMHSVAVCALMIALAKELNLNDTETKQAGMAGLLHDVGKAGIPLEVLNKPGA
                     LTDDEFTLVKLHPERGHAMLMQANILDEVVLDVCLHHHEKVNGSGYPHKLAADNISLF
                     AKMGAVCDVYDAVTSNRPYKAGWEPGVSLHRMAQWEGHFDDVVFKAFVRSIGIYPIGS
                     MVKLKSGRLAVVVDQNEKSLLTPLVKVFFSTKSKTRIPVETLDLSKPSANDSIASHED
                     PATWGIHNMQEIWA"
     gene            2054..3022
                     /gene="thiL"
                     /locus_tag="PKL42_09150"
     CDS             2054..3022
                     /gene="thiL"
                     /locus_tag="PKL42_09150"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNU67280.1"
                     /translation="MSPEFNLIQQYFTRPSHHTKLGVGDDAALISPSAGMELAISADM
                     LVADTHFFSDADAYSIGWKSLAVNVSDMAAMGANPKWVTLAIALPEINHAWLAEFSRG
                     LFACAKEFDVDLIGGDTTRGPLTISVQIMGEVPAGQAIKRSGAKLEDDIWVSGCIGAA
                     ATGLAHLQKKVVMPAGELQIGLAALHTPQPRVALGLALSTIANSCIDISDGLLADLGH
                     ILEASQLSARLDLALIPSLDFIHHNLQQPDFQHYLLAGGDDYELCFTAAKSSREKIVQ
                     LSQTLGLPLTRIGATCQSTTDKQAHIIVEYQQQAIHIAKKGFDHFD"
     gene            3027..3518
                     /locus_tag="PKL42_09155"
     CDS             3027..3518
                     /locus_tag="PKL42_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HNU67281.1"
                     /translation="MSTQPDFKFLKAHLAHFFALGFGSGLAPKAPGTVGTLVAFPLFW
                     LIRDFSLGYQFAFVTVLFGIGIYFCSKTSLSLGVADHGSIVWDEIVAMMLVLTFTPRH
                     WAFWILAFVLFRLFDIWKPFPIKQCDAKLKNGFGVMFDDILAAIYAVICMQVAVLWLI
                     SKH"
     gene            3497..4003
                     /locus_tag="PKL42_09160"
     CDS             3497..4003
                     /locus_tag="PKL42_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HNU67282.1"
                     /translation="MVNLETLAAELGAKLNANELTLTLAESCTGGMVAEAITRIAGSS
                     AWFDRAYITYSNTAKIEMLGVSALTLNQFGAVSEQTAQEMAVGALKHSHAQIAGSITG
                     IAGPDGGSSEKPVGTVCFAWVGHHFAAQTTTQHFKGNRQSIRQQAAAAMLTGLIDRLI
                     ATEFNKTN"
     gene            complement(4000..>4231)
                     /locus_tag="PKL42_09165"
     CDS             complement(4000..>4231)
                     /locus_tag="PKL42_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67283.1"
                     /translation="GYSSNYSLGYSNYPNVVYYGAPVVYHQPIVHYVPVTSYSNGHYY
                     GNRNHRGHGHHGHRGHERSYQNRGHDGGHHRR"
BASE COUNT         1152 a          991 c          990 g         1098 t
ORIGIN      
        1 gtgcaagtag cggtatttat gaagataaag gcattcctaa tttgcagtca tggttagaac
       61 ttgctgttaa atcacccttt tcgctcgaaa aaagactgat ctcagacgag caaagcctta
      121 ttgaaagcac tttagttgac ttagttgata atcaagcttg ccacctcatc ctcaccacag
      181 gtggcactgg gcctgccttg cgtgatgtga cccctgaagc cacattagcc attgccgata
      241 aaaccatgcc cggttttggc gagcaaatgc ggcaaatcag cctcaatttt gtgcctacgg
      301 ctattctctc gcgccaagtt gcagtggttc gcaagcaatg tttaattatc aacttacctg
      361 gtcagcccaa ggccattgcc caaacattac aaggcttaaa agatgagacg ggtaatacaa
      421 aagtgcacgg catattttcg gcggtgcctt attgtctcga cttgctcggc acgccagaaa
      481 aacccatgcc gtatcttgaa acgcacgagc aaatcgtcaa ggcgtttagg ccaaaatcaa
      541 gctaagcgct ttcaatttca attgcactaa ttacaggggt tttaccttgt tgtttaacct
      601 atcaaaagca gccaattgca tgcaaaatag gcacaattat gttaaagaca attccaactc
      661 atcaagtcac actcggtatg cacattcatg ccctcaacgg cgcgtggatt gaccatccat
      721 tttggaaatc aaaatttgta ctcaatgacc ctgaggattt aaaaaaactt agagccagta
      781 gcattaaaga agtggtgatt gatattgcca aaggtgtgga tgtcaaaacc agtgaaacag
      841 ccaccactgc acccgcagct acgcagcctg cggaatcggt tgctgaacaa ccccaaaccc
      901 ctatagaaaa aaaagctgtt gcgcgtgttt cggctgctga agaacaagtg cgtgctaaaa
      961 aaatcctgca agaatctaaa aaagccgtca cgaccatgct caatgatgtc cgcatgggca
     1021 aggcagtgaa ccccggcaca gcaacagcgt tggtggaaga aattgcctcg tcagtctcac
     1081 gcaatgaaag cgcgctcatt agcttagtgc gtcttaaaac caaagacgat tacacttaca
     1141 tgcactcggt tgccgtatgc gcgttgatga ttgcgctggc aaaagaactt aatttaaacg
     1201 acactgaaac caagcaagcg ggcatggcag gattgttgca cgatgttggc aaagcgggta
     1261 ttccgctcga agtacttaac aagccaggtg cgttgaccga tgacgagttt accttagtca
     1321 aattgcatcc tgaacgtggg cacgctatgc tcatgcaagc caatatcttg gatgaagtgg
     1381 tgttagacgt atgcctgcac caccatgaaa aagttaacgg ctcaggttac ccacacaagc
     1441 tcgcggcaga caatattagc ttattcgcca aaatgggcgc ggtgtgcgat gtatatgacg
     1501 ccgttacatc aaaccgcccc tataaagctg gctgggaacc tggtgtatca ctgcatcgca
     1561 tggcgcagtg ggaaggccat tttgatgacg ttgtgtttaa agcttttgtc agaagcatcg
     1621 gtatttatcc aatcggctct atggtcaagc ttaaatctgg cagactggct gtggtcgttg
     1681 atcaaaatga aaaatcactg ctcaccccat tggtcaaagt atttttctca acaaaatcca
     1741 aaacgcgcat ccccgttgaa acgctggatt tatcgaaacc gagcgccaat gacagcatcg
     1801 ccagccatga agacccagcc acttggggca ttcataacat gcaagaaatt tgggcttaat
     1861 ccttcgtaaa tagttacaaa ccttccgcac tgccctgcaa gccttataaa taaaggcttg
     1921 cagggcagct ttttttgctc gggtaattta ttgcgctaaa aacaagtttt ttcagccata
     1981 aattcgcatg ttttgtcgcg cgtatcccat gtaaaatgcg tgtattaaaa tgaagcaaat
     2041 gacaccgcaa cagatgagtc cagaatttaa cttaattcag cagtatttta cgcgcccctc
     2101 acaccacacc aaattagggg tgggcgatga tgctgcgctg atttcaccaa gcgcagggat
     2161 ggaacttgct atttctgcag atatgttggt ggccgatacg catttttttt ccgatgctga
     2221 cgcttattcc attggttgga aatcgctcgc tgttaacgtg agtgatatgg ccgccatggg
     2281 cgccaaccca aaatgggtaa cattggcaat tgccctgcca gaaatcaacc atgcttggct
     2341 ggctgaattt tcacgcgggc tgttcgcttg tgccaaagaa tttgatgtgg atttaattgg
     2401 tggcgatacc acgcgcggcc ctttaacgat tagcgtgcaa attatgggag aagtgccggc
     2461 aggccaagca atcaaacgaa gtggcgcgaa actagaagat gatatttggg tgtcaggctg
     2521 tataggcgct gccgctactg gcctggcgca cttacaaaaa aaagtggtaa tgcccgctgg
     2581 cgaactgcaa atcggcttag ccgccctgca tactccgcaa ccgcgtgtgg cattaggttt
     2641 ggcattaagc accatcgcca atagctgcat tgatatttca gatggattac tggcagattt
     2701 aggccacatt ttagaagcct cacagttgag cgcccgcctc gatttggcct taattccaag
     2761 tctggatttt attcaccata atttacagca gcctgatttt cagcactatc tattggctgg
     2821 tggcgacgat tatgaattat gctttaccgc agcaaaatct agccgcgaaa aaatagtaca
     2881 attaagccaa acactcggtt taccgctcac tagaattggc gcaacctgcc aaagtacaac
     2941 tgacaaacaa gcgcatatca tcgttgaata tcaacagcaa gccatccata ttgcaaaaaa
     3001 aggttttgac cattttgact aacccaatga gcacgcaacc tgattttaaa tttttaaagg
     3061 ctcaccttgc gcatttcttt gcgcttgggt ttggcagtgg attagcaccc aaagcgcccg
     3121 gcaccgtggg tactttagtt gcttttccac tcttttggtt aattcgtgat ttttcacttg
     3181 gctatcagtt tgcttttgta acggtgctct ttggcattgg catctatttt tgcagtaaaa
     3241 ccagcctgtc gctgggggta gcagatcacg gcagtatcgt gtgggatgaa attgtcgcca
     3301 tgatgctggt gctgactttt acgccgcgcc attgggcttt ttggattctg gcgtttgtgt
     3361 tgtttcgtct atttgatatt tggaaacctt ttcccattaa gcaatgcgac gctaaactta
     3421 aaaatggctt tggcgtgatg tttgacgaca ttctcgcagc gatttacgca gttatttgca
     3481 tgcaggttgc ggtgttgtgg ttaatctcga aacattagcg gccgaattgg gcgcaaagct
     3541 caatgcgaat gaattgacgc tgactcttgc ggaatcatgc acgggcggca tggtagcaga
     3601 agcgattacc cgcattgctg gcagttctgc ttggtttgac cgtgcttata ttacctatag
     3661 caatactgcc aaaattgaaa tgctgggcgt gtcagcactt accttaaatc agttcggcgc
     3721 ggtgagcgag caaaccgcgc aggaaatggc tgttggcgca cttaaacaca gccatgcaca
     3781 aatcgcaggg agcattactg gcattgcagg acccgatggc ggtagctcgg aaaaacctgt
     3841 gggtacggtc tgttttgcat gggttggcca tcactttgca gcacaaacca ccacgcaaca
     3901 ctttaaagga aaccgtcaaa gtatccgcca acaagccgca gccgcaatgc tgactgggct
     3961 tatcgacaga ttaatagcga ccgagtttaa taaaacaaat taacgacggt gatgcccacc
     4021 atcatgtccg cgattttgat agcttctttc atgtccgcga tggccgtggt gaccgtgacc
     4081 acgatgatta cgattgccat aataatggcc attactgtaa ctcgtgaccg gtacatagtg
     4141 cacgatgggt tgatggtaaa ccacaggtgc gccgtaatac accacattcg gatagtttga
     4201 atagcctagc gagtaattgg atgagtaacc a
//
LOCUS       DAOQSI010000168         4137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_23302_length_4137_cov_4.494121, whole genome shotgun sequence.
ACCESSION   DAOQSI010000168 DAOQSI010000000
VERSION     DAOQSI010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23302_length_4137_cov_4.494121"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="PKL42_09170"
     CDS             <1..1008
                     /locus_tag="PKL42_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNU67284.1"
                     /translation="KTYDLIAPKVGIFIGNMALSDPRFTELYSMAEAIGGISGAKGGI
                     LPAAANSTGMHMIGVMPNGTGMHARAMLEIPRKAYLLLNIEPELDCQHAALAKAAMEK
                     AECVVALTSFKSQALEYADILLPIAPFTETSGTYMSMEGRVQSFAAAVKPLGECRPAW
                     KVLRVLANTLGLQGFDFETSEEVKDKLFEGEKPSTVVWRSLNNNLKELVEIQVNIKHD
                     GLQRIGEVPQNESDPIVRRAASLQKTKYNIRPMARMRAEQIAALGLVEGDIVLAKQDK
                     GSAVLKVKLDNHVAMGCVRVAASHELTTSLGDLMGDITLEKTTDTSYKPYDTVIMEAR
                     G"
     gene            1005..2057
                     /gene="nuoH"
                     /locus_tag="PKL42_09175"
     CDS             1005..2057
                     /gene="nuoH"
                     /locus_tag="PKL42_09175"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNU67285.1"
                     /translation="MIAMFEPLLNNLASVFGSYWPAVQLTGWTLIKIIAIVAPLMGAV
                     AYLTLAERKVIGYMQVRIGPNRVGYFGLLQPIADGIKLLMKEIVLPTASNKTLFLIGP
                     VLAIAPAFAAWAVVPFDATLVLADINAGLLYILAMTSVAVYGVIIAGWASNSKYAFLG
                     SLRSAAQIVSYEIAMGFALVGVIMCANSLNLGKIVVGQTGGFWHWYFIPLFPLFVVYF
                     ISAVAETNRAPFDVAEGESEIVAGFHVEYSGMAFAVFFLAEYANMWLVATLAALMFLG
                     GWLSPVPFLPDSFLWLLAKVGFLLFLFLWFRATFPRYRYDQIMRLGWKVFIPITIVWI
                     VFIGGMMQTPLAYLFH"
     gene            2077..2568
                     /gene="nuoI"
                     /locus_tag="PKL42_09180"
     CDS             2077..2568
                     /gene="nuoI"
                     /locus_tag="PKL42_09180"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HNU67286.1"
                     /translation="MISKIKNTLSSLMLGELLKGMALTGRYFFAPKITVQYPEESTPQ
                     SNRFRGLHALRRYPNGEERCIACKLCEAVCPAMAITIESEQREDNTRRTTRYDIDLTK
                     CIFCGMCEESCPVDSIVETRIFDYHGEQRGDLLYTKDMLLAVGDKHEKQIAADRAIDK
                     QFR"
     gene            2597..3256
                     /locus_tag="PKL42_09185"
     CDS             2597..3256
                     /locus_tag="PKL42_09185"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNU67287.1"
                     /translation="MTFFGLPFHDIVFYVLATILLYAAVSVITTRNPVYAALYLVLAF
                     FTAAGIWLLLEAEFLAITLVLVYVGAVMVLFLFVVMMLDINLDKLREGFWKALPVALP
                     VGGLMAIEMVMIVGTRNFGVDKVATPAAKSADYSNTAELGRVLYTDYLLPFELASIVL
                     LVAIVAAIALTLRGRRESKSMDPAKQVLVKKSDRLRVVKMDAIVEHAAEKNDAPLKED
                     K"
     gene            3256..3561
                     /gene="nuoK"
                     /locus_tag="PKL42_09190"
     CDS             3256..3561
                     /gene="nuoK"
                     /locus_tag="PKL42_09190"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNU67288.1"
                     /translation="MVGLSHYLVLGTLLFAISVIGIFLNRKNVIILLMAIELMLLAVN
                     LNFIAFSHYLHDIAGQVFVFFILTVAAAESAIGLAILVVLFRNLRTINVDDLDTLKG"
     gene            3571..4137
                     /locus_tag="PKL42_09195"
                     /pseudo
     CDS             3571..4137
                     /locus_tag="PKL42_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441516.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
BASE COUNT         1067 a          739 c          995 g         1336 t
ORIGIN      
        1 aaaacttatg atttaattgc accaaaagtg ggtattttta ttggaaatat ggccctgagc
       61 gatccgcgat ttactgaact gtatagtatg gctgaagcga taggcgggat ctccggtgcc
      121 aaaggcggca ttttgccagc ggccgcaaat agtaccggca tgcatatgat tggggtgatg
      181 ccaaatggca caggcatgca tgctcgtgca atgttggaaa ttccaagaaa agcttacttg
      241 ctactaaaca tcgagcctga attagattgt cagcatgctg ctttagcaaa agccgcgatg
      301 gagaaggctg aatgcgtagt ggcattaact tcatttaaat ctcaagcatt agaatatgca
      361 gatatattgt tgccaattgc gccttttaca gaaacttctg gaacttacat gagcatggaa
      421 gggcgcgtgc agagttttgc ggccgcagta aaaccattag gcgaatgccg accagcttgg
      481 aaagtattac gtgtgttagc gaacacatta ggattgcaag gttttgattt tgaaacgagt
      541 gaagaagtca aagataaact ttttgaaggt gaaaaaccct ctacagttgt ttggcgtagt
      601 ctaaataaca acttaaaaga gcttgtagag attcaagtaa acattaagca cgatggcttg
      661 cagcgcatcg gcgaagttcc acaaaatgaa tctgatccaa tcgtacgtcg tgcggcatcg
      721 ttgcaaaaaa ccaaatataa cattaggcct atggcgcgta tgcgtgctga acagatagcc
      781 gctttaggtt tagtagaggg tgatattgtc ctggctaaac aagataaagg cagtgcggtg
      841 ctgaaggtta aactggataa tcatgtggcg atgggttgcg ttcgagtagc tgcctctcat
      901 gaactcacaa ccagcttagg tgatttaatg ggtgatatca cgcttgaaaa aacaactgac
      961 accagctata agccatatga cacggtgata atggaagcgc gcggatgata gccatgtttg
     1021 aacctttatt aaataactta gcgagcgtct tcggcagtta ttggcctgcc gttcagctta
     1081 ccggctggac tttaattaaa attattgcga ttgtcgcacc attgatgggt gccgttgcct
     1141 atttaaccct tgcagaacgt aaagtaattg gctacatgca ggtgcgcatt ggccccaatc
     1201 gggtaggcta ttttgggttg ttacagccaa tagccgatgg cattaaattg ctgatgaaag
     1261 agattgttct tccaacagca tcgaataaaa ctttattttt aattggccca gtactggcca
     1321 ttgcccctgc ctttgcagca tgggcggtcg ttccttttga tgccacttta gtgcttgcgg
     1381 atattaatgc aggcttgctc tacattttag cgatgacctc tgttgccgtt tatggggtga
     1441 ttattgctgg ctgggcatcg aattcaaaat atgcattttt aggtagtttg cgctcggcag
     1501 cacaaatcgt ttcatatgaa attgcaatgg ggttcgcttt ggtgggcgtg attatgtgcg
     1561 cgaactcact taatcttggc aaaatagttg tgggtcaaac tggtggattt tggcactggt
     1621 atttcattcc attatttcca ttatttgtcg tttatttcat tagtgcagtc gctgaaacaa
     1681 atcgcgcgcc tttcgatgtg gctgaaggtg agtcagaaat cgtcgcaggt ttccatgttg
     1741 aatattcagg catggccttc gcggtatttt tcttggctga gtatgccaat atgtggctag
     1801 tcgctacatt ggccgctttg atgtttttag gtggttggtt atctccagtg ccatttttac
     1861 cagatagctt tttatggctg ctagccaaag ttggcttttt attattttta tttttatggt
     1921 ttagagctac ttttccacga tatcgatacg atcagattat gcgtttagga tggaaggtct
     1981 ttattccaat tacgattgtg tggattgttt ttattggcgg tatgatgcaa acaccactgg
     2041 catacttgtt tcactaaatt tgatgcctag aattttatga taagcaaaat aaaaaataca
     2101 ttatcaagct taatgcttgg ggagttgctc aaagggatgg cgcttacagg gcgttacttt
     2161 tttgcgccca aaattactgt gcagtatcca gaagaaagta caccacagtc taaccgcttt
     2221 cgtggtcttc acgcgcttcg caggtatccg aacggggaag agcgctgtat tgcatgtaag
     2281 ctgtgtgaag cagtttgccc agcgatggca attaccattg aatcagaaca acgtgaagat
     2341 aacactcgcc gcacaacacg ttatgatatt gatctcacaa agtgtatttt ttgcggaatg
     2401 tgtgaagagt cttgtcccgt agactcgatt gttgaaacgc gtatttttga ttatcacgga
     2461 gagcagcgtg gtgatttgct ttataccaaa gacatgctat tggccgttgg tgataagcat
     2521 gaaaaacaaa tcgctgcaga ccgtgcaatt gataagcagt ttagatagaa atttttaagg
     2581 tttgatgagt aaataaatga cattttttgg cctacctttt cacgatatcg ttttttacgt
     2641 actagctacg atattgctgt atgcggcagt aagcgtaatt actacgcgaa accctgtgta
     2701 cgctgctctg tatttggtat tggcgttttt tactgctgca ggaatatggc tattgttaga
     2761 ggctgaattt ctagctatta cgttggtgtt ggtctatgta ggggcggtca tggtgttatt
     2821 tttgtttgta gtcatgatgc tcgatatcaa cttagataag ttacgtgaag gcttttggaa
     2881 agccttgcca gttgcgctgc ctgttggtgg tttaatggca attgaaatgg tgatgattgt
     2941 tggaacccga aactttggtg tggataaagt tgccacgcca gctgcaaaaa gtgctgatta
     3001 tagtaatact gccgaattag gccgtgtgtt atatacggat tatttacttc catttgaatt
     3061 ggcctcaatc gtattgttgg tggctattgt tgcggctatt gcactcactt taaggggacg
     3121 tcgtgaaagc aaatcgatgg atcctgcgaa acaagtgtta gtgaaaaagt cagatcgatt
     3181 aagagtcgtg aaaatggacg ccattgtaga gcatgctgca gaaaaaaatg atgcgccttt
     3241 aaaggaggat aagtaatggt ggggctttca cattaccttg ttttgggtac attgctattc
     3301 gctattagcg tcattggcat cttcttaaac cgtaaaaatg tcatcatttt attgatggct
     3361 atagagttga tgttattagc ggttaacctt aatttcattg ctttttctca ttacttacat
     3421 gatattgcag gccaagtgtt tgtgtttttt atacttactg tggctgccgc ggaatctgcg
     3481 ataggtttag ctatattggt ggtgttgttt agaaacttgc gcacgattaa tgttgatgat
     3541 ttagatactc taaaaggcta atatttacca atgacaatgc aacagattta tttagccata
     3601 cctctactgc ctttgctggc agccattttg gtaggcttat tcggcaaact catgccgcgt
     3661 tcaatggcac atattctaac cgtagcgggt gttgcagttt catttgcttt atcgctttat
     3721 gtgttgaaaa gtgccttggt gagcgaaccc tataatgcta cggtatattc atggttaaag
     3781 agtggaagct ttacttttga gattgggttt ttaattgacc gattatcagc aatgatgatg
     3841 gtagtggtca cttttgtttc cctgatggtg catatttaca ccattggtta catgcatgat
     3901 gacaagggtt acgctagatt tttcagctat atctccttgt ttacttttgc catgttgatg
     3961 ttagatgtcg aataacttca tgcagttatt ttttggctgg gaggcagtgg gtttagtttc
     4021 atacttactt atcggatttt ggtatacacg tccatcggcc atctacgcta atttaaaagc
     4081 ttttttagta aaccgcgtgg gtgattttgg atttttgctg ggcataggcc tagtgtt
//
LOCUS       DAOQSI010000169         4029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_24212_length_4029_cov_5.985657, whole genome shotgun sequence.
ACCESSION   DAOQSI010000169 DAOQSI010000000
VERSION     DAOQSI010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24212_length_4029_cov_5.985657"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /gene="asd"
                     /locus_tag="PKL42_09200"
     CDS             complement(<1..479)
                     /gene="asd"
                     /locus_tag="PKL42_09200"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167872.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HNU67289.1"
                     /translation="MKLAVLLQYLMPKQAITALAGKLAHLEGGNTTTAVIRWFVKRYQ
                     VNMNEAANPDISSYRTFNEFFTRPLKAGARPIAKAKFICPVDGAISQFGKIEQDQIFQ
                     AKGHTYSTTALVGGNQLLANQFENGHFACLYLSPKDYHRNHMPCDGKLKSMTYVPGD"
     gene            complement(581..1633)
                     /gene="ilvC"
                     /locus_tag="PKL42_09205"
     CDS             complement(581..1633)
                     /gene="ilvC"
                     /locus_tag="PKL42_09205"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNU67290.1"
                     /translation="MSRNLIQFTEQIMKVYYDKDADLSLIKGKKVTIVGYGSQGHAHA
                     ANLKDSGANVTIGLRKGGSSWAKAVAAGHNTLEIADAVKQADVVMVLLPDETMAAIFD
                     AEIAPNLQKGAALAFAHGFNIHYNQIVPRADLDVIMIAPKGPGHTVRSEYLKGGGVPS
                     LIAVYQDKSGKAKDIALSYAAANGGTKGGVIETDFREETETDLFGEQAVLCGGAVELV
                     KAGFETLVEAGYAPEMAYFECLHELKLIVDLMYEGGIANMNYSISNNAEYGEYVTGPK
                     VINDESRYAMQECLANIQNGNYAKQFILEGRTNYPEMTARRRLNAAHPIEQVGGQLRA
                     MMPWIAKNKLVDQSKN"
     gene            complement(1716..2207)
                     /gene="ilvN"
                     /locus_tag="PKL42_09210"
     CDS             complement(1716..2207)
                     /gene="ilvN"
                     /locus_tag="PKL42_09210"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNU67291.1"
                     /translation="MRHIISLLMENESGALSRVSGLFSARGYNIESLTVAATEDPTLS
                     RMTIVTAGSDEVIEQIIKQLNKLIDVVKVLDLNDGKFIERELMLVKVKATGAFREEMK
                     RMCDIFRGRIIDVADGSFTIELTGSGTKLDAFIESLDKAAILETVRTGASGIGRGDRI
                     LKV"
     gene            complement(2207..3937)
                     /locus_tag="PKL42_09215"
     CDS             complement(2207..3937)
                     /locus_tag="PKL42_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 catalytic subunit"
                     /protein_id="HNU67292.1"
                     /translation="MANTQKITGADIVIRCLEQEKVKFVFGYPGGAVLHIYDAIYNQD
                     YFKHILVRHEQAAVHAADAFSRATGEVGVALVTSGPGATNAITGIATAYMDSIPLVVI
                     SGQVPVPAIGLDAFQEVDMVGVTRPCVKHNFLVKDVKDIALTMKKAFHIARTGRPGPV
                     VVDIPKDITAHLSEFIYPDTVEMRSYNPVTKGHSGQIKKAVKLLLEAKRPMVYTGGGV
                     VLGDASEALVKLVRALGVPVTNTLMGLGGYPSTDPQFVGMLGMHGTYEANMAMQDCDV
                     LLAVGARFDDRVIGNPKHFFNKSRTIIHIDIDPSSISKRVKVDVPIVGDVKSVLAEMN
                     ELVAAENPKQSSALKAWWAQIDSWRSKLCLDYDNSNGLIKPQYVIQKLWEVTKGEAYV
                     TSDVGQHQMWAAQYYKFDKPRRWINSGGLGTMGVGLPYAMGCQFADPSAQVACVTGEG
                     SIQMNIQELSTCKQYHLPIKVILLNNRYLGMVRQWQEFFYENRYSESYMDSLPDFVKL
                     AESYGHVGMQITQPGDVEGALKEAFAMKSRFVFMDFITDQKENVFPMIEAGKGLSEMI
                     LANDIGAEDL"
BASE COUNT         1010 a          960 c          896 g         1163 t
ORIGIN      
        1 aagtcgcctg gcacgtaggt catacttttt aatttgccat cgcaaggcat gtggttgcga
       61 tggtaatctt ttgggcttaa atacaggcag gcaaagtggc cattttcaaa ttgatttgcc
      121 agcaattgat tgccaccaac tagcgctgtt gttgaataag tgtggccttt ggcttgaaaa
      181 atctggtctt gttcaatctt tccaaactgg ctgatggcac cgtccacagg gcaaataaac
      241 ttagcttttg caatcggcct cgcacctgct tttaatgggc gcgtgaagaa ctcgttaaat
      301 gtgcggtagc tcgaaatgtc ggggttagca gcctcattca tattgacctg atagcgcttc
      361 acaaaccagc gtatcaccgc ggtggtggta ttgccgcctt ctaaatgggc gagcttgccg
      421 gccagcgcgg taatggcttg tttgggcatt aagtattgta ataaaacagc gagcttcata
      481 ctttttttcc taataaaaaa gcggggtgct gatttagcat cccgcctaat ttacatcggc
      541 tgaactagat tagttcttcg attgtgacat ttaatgttga tcaattcttc gattgatcta
      601 ctaatttatt tttcgcaatc caaggcatca tggcgcgtaa ttggccgccc acttgctcaa
      661 ttgggtgcgc tgcatttaaa cgacgacgcg cagtcatttc tgggtagtta gtgcggcctt
      721 ctaagatgaa ttgttttgca tagttgccgt tttgaatgtt ggccaaacat tcttgcatgg
      781 cgtaacgtga ctcatcatta atcactttag ggccagtcac atattcaccg tattccgcgt
      841 tgtttgagat tgagtagttc atgttggcaa tgccgccttc gtacatcaaa tcaacaatca
      901 atttcaattc gtgcaagcat tcaaagtagg ccatttctgg cgcgtaacct gcttcaacta
      961 aagtttcaaa gccggctttt actagctcaa ccgcgccacc acagagtact gcttgttcgc
     1021 cgaacaagtc cgtttcagtt tcttcacgga aatccgtttc aatcacgccg cctttagtgc
     1081 cgccgttcgc agcagcgtaa gaaagagcga tgtctttggc tttacccgat ttatcttgat
     1141 aaactgcgat taaagaaggc acaccgccac ctttaaggta ttctgaacgc acagtgtggc
     1201 ctgggccttt aggggcaatc ataatcacgt ctaaatctgc gcgtggcacg atttggttgt
     1261 agtgaatgtt aaagccgtga gcaaatgcca acgctgcgcc tttttgtaag tttggtgcga
     1321 tttccgcatc aaaaatggct gccatggttt cgtctggcaa taacaccatt acaacatccg
     1381 cttgtttcac tgcatcagca atttctaatg tgttgtggcc agcagccacg gcttttgccc
     1441 aagagctgcc acctttgcga aggccgatag tgacgtttgc gccgctatct tttaagtttg
     1501 cagcgtgtgc atggccttgt gaaccgtaac ctacaatcgt tactttttta cctttgatta
     1561 aactaaggtc agcgtctttg tcgtaataaa ctttcattat ttgctccgta aattgtatta
     1621 aatttcgtga aatgtgaaat gtgaaatatg aaatgtaaca accacgttgg gttaatacgt
     1681 ttcacttttc acctttcact ttttacattg actgattaaa cttttaaaat tcggtcgccg
     1741 cgcccgatgc ccgatgcgcc agtgcgtacg gtttcgagga ttgcagcttt atctaaactt
     1801 tcgataaaag cgtctaactt ggtgcctgag ccggtaagct caattgtaaa actaccatca
     1861 gccacatcga taatgcggcc gcggaatata tcgcacatgc gtttcatttc ttcacgaaac
     1921 gcacctgtgg cttttacttt aaccaacatc aattcacgtt caatgaattt tccgtcgttt
     1981 aaatcaagca ctttcacaac atcaatcagc ttattcagct gtttgatgat ttgttcaatc
     2041 acttcgtccg agcctgccgt tacaatggtc atgcgtgaca aagtggggtc ttcagttgca
     2101 gccacggtaa gggactcaat attgtaaccg cgcgcagaaa ataaacctga tacgcgtgaa
     2161 agtgcgcctg attcgttttc cattaacaat gaaataatat gacgcattat aaatcctccg
     2221 caccaatatc attcgccaaa atcatctcag acaaaccttt gcctgcctca atcattggga
     2281 atacattttc tttttggtcg gtaataaaat ccataaatac aaaacgcgac ttcatggcaa
     2341 atgcctcttt taatgcgcct tcaacgtcac ccggttgcgt aatttgcatg ccaacatggc
     2401 cgtaagattc tgccagtttc acaaagtcag gcaggctatc catgtaagac tcggagtaac
     2461 ggttttcata aaagaactct tgccattgac gcaccatgcc taaatagcgg ttattgagca
     2521 aaatcacttt aatcggcaag tggtattgct tgcaggttga aagctcttga atattcattt
     2581 gaatactgcc ttcacccgtc acacaggcca cttgcgcgct tgggtcggca aattgacagc
     2641 ccatcgcgta cggtaagcca acgcccatcg tgccgaggcc gcccgagtta atccagcggc
     2701 gcggtttgtc aaacttgtag tattgcgctg cccacatttg gtgctgacca acgtcggacg
     2761 tcacataggc ttcgcctttg gtgacctccc acagtttttg aatcacatac tgcggtttga
     2821 tcaagccgtt gctattgtcg taatcaaggc atagtttgct gcgccatgag tcaatttgcg
     2881 cccaccatgc ttttaaagcg gaagattgct tggggttttc agcagcaaca agctcgttca
     2941 tttcagccag cacagacttc acatcaccta caatcggtac gtctactttc acccgttttg
     3001 agatggaaga ggggtcaata tcgatgtgaa taatggtgcg agacttatta aaaaagtgtt
     3061 tggggttgcc aatcacgcgg tcgtcaaagc gtgcgccaac cgccagcaac acatcacagt
     3121 cttgcatcgc catattggct tcataagtgc cgtgcatgcc gagcatgccg acaaattgcg
     3181 ggtcggtaga agggtagccg cctaggccca ttaaggtatt ggtgacaggc acgcctaatg
     3241 cgcgcacaag ctttacaagc gcctcagagg catcacccag cactacaccg ccaccggtgt
     3301 agaccatcgg gcgtttcgct tcaagcagta atttaactgc ttttttgatt tgtccgctgt
     3361 ggcctttagt cactgggttg tatgagcgca tttcaaccgt gtctgggtaa ataaattcgc
     3421 tcaaatgtgc cgtaatgtct ttaggaatat ccaccactac cgggcctggg cggcctgttc
     3481 tggcaatgtg aaaagccttt ttcatggtga gggcaatgtc tttaacatct ttcaccaaaa
     3541 agttatgttt tacgcatggg cgcgtcacgc caaccatgtc tacctcttgg aaggcatcta
     3601 aaccaatggc cggtaccggc acttggccgc tgatgaccac cagtggaata gagtccatat
     3661 aggccgtggc aatgccggta atggcattgg ttgcccctgg gcctgatgtg accagtgcca
     3721 cacccacttc acccgtagcg cgtgaaaaag catcggccgc gtgcacggcg gcttgctcgt
     3781 gacgcactaa aatatgctta aagtaatctt gattgtaaat cgcgtcgtag atgtgtaaga
     3841 ctgcgccacc aggatagcca aatacgaatt tgactttttc ctgctccaaa catcgaatta
     3901 caatatctgc gccagtgatt ttttgagtgt ttgccataaa acttatatgc ttaaatttat
     3961 taattttatt gattatattg gtaaccctgt agattaacgg tttgaggcta tttggtcaag
     4021 gaacatagc
//
LOCUS       DAOQSI010000170         4016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_24343_length_4016_cov_5.720273, whole genome shotgun sequence.
ACCESSION   DAOQSI010000170 DAOQSI010000000
VERSION     DAOQSI010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24343_length_4016_cov_5.720273"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PKL42_09220"
     CDS             complement(<1..395)
                     /locus_tag="PKL42_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HNU67293.1"
                     /translation="MLTALYIMLGLALVLGLGLGYAALKFKVEGDPLIARIDAILPQT
                     QCGQCGYPGCKPYATAIANGEADINQCPPGGDAGVHALADLLGVEYKPLNAEHGAPKP
                     KSVAFIDEKTCIGCTLCIQACPVDAILGAA"
     gene            complement(404..997)
                     /gene="rsxA"
                     /locus_tag="PKL42_09225"
     CDS             complement(404..997)
                     /gene="rsxA"
                     /locus_tag="PKL42_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HNU67294.1"
                     /translation="MTFNLQHYFMILIGTVLVNNIVLVKVLGLCPFMGVSKKLETSLS
                     MAGATAFVLSIGSMTSWGVNHYLLEPNNLFYLRTLSFIVIIASVVQLTEMVMEKNFPP
                     LYQGLGIFLPLITTNCAVLGIPLLNAQASHSFLESLLFGLGGALGFSLVLILFASMRE
                     RLEAADVPKVFQGSAIAMVTAALMSLAFMGFAGLDRY"
     gene            1236..2120
                     /gene="dapA"
                     /locus_tag="PKL42_09230"
     CDS             1236..2120
                     /gene="dapA"
                     /locus_tag="PKL42_09230"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNU67295.1"
                     /translation="MLQGSLVAIVTPMFEDGRLDISSLRALIDFHLAEGTDGIVIVGT
                     TGESPTVDFDEHCLLIETAVSQVNGRVPVIAGTGANSTKEAIVLTQKAKELGADACLL
                     VAPYYNKPTQEGLYQHFKAVADAVEIPQILYNVPGRTGCDISNDTTLRLSSHRNIVGI
                     KDATGGIERGTDLLLRAPKDFVVLSGDDATALSLMLLGGKGVITVTGNVAPKLMHEMC
                     EAALKGDVATARAINAKLFALHQKLFVEANPIPVKWVLAQMGLIKTGIRLPMVQLSAQ
                     YHEVLRDACKPAELLSSP"
     gene            2165..3439
                     /gene="bamC"
                     /locus_tag="PKL42_09235"
     CDS             2165..3439
                     /gene="bamC"
                     /locus_tag="PKL42_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HNU67296.1"
                     /translation="MKQLNKTNMIKHTAIYALLLSSLAACDSIPFIDNSSDYKGAGRS
                     KPLEVPPDLTASPVSDAYSIPGSTSYSTYSQAQEGQEAGVEKVLATPEGVRLVRAGAQ
                     RWLVVNAPAEKVWPVIREFWTDQGFAVRKEDAQLGVMETEWIDSDAIKKDTSGNIGDK
                     FDKWLDKLSGFADKKKFKTRLDHGTEPDTTEIYMTHRSVAGAPDDGKNRVQTQLGEID
                     TGYKADAKKAPTADARDDELDAELLRRLMVKLGVEEQKSKAVMANTVEIKRAEVVKEK
                     DGSATLYVNDPFDRAWRRVGLALDRIGFVIEDKNRSEGVFYVRYADVDIDDSPKKKKG
                     LLETLKFWGDDDDKKEPETAAKKEEKSIMDSLKFWGKDDKKSGNLEKQYRIKVADGES
                     DKTSVTVVDKDGARVGTTTANRIVALMYEQLR"
     gene            3449..>4016
                     /locus_tag="PKL42_09240"
     CDS             3449..>4016
                     /locus_tag="PKL42_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNU67297.1"
                     /translation="MRFASLGSGSAGNALLVEVNRTTVMLDCGFSIKETVARLARLNV
                     QPEGISGIVVTHEHDDHAGGVFKFAAKYQIPVWLTYGTHKMVSRYMPNQHQLQLNIIN
                     SHQAFAIDAIEVQPYPVPHDAREPIQCLFSDGQMQLGVLTDVGRTTPHIEENLRNCHA
                     LVLECNHDANMLKTGPYSWPLKQRVGGEL"
BASE COUNT         1153 a          874 c         1016 g          973 t
ORIGIN      
        1 gctgcaccca aaattgcgtc caccgggcag gcttgtatgc acaaggtgca gccaatgcag
       61 gttttttcat caataaacgc aacggattta ggtttaggcg cgccgtgttc agcgttaagc
      121 ggtttatatt ccacgcctaa taaatctgcg agcgcatgga cccctgcatc gccgccaggc
      181 gggcattggt tgatatcggc ttcgccattg gcaatggctg ttgcataggg tttgcaaccg
      241 gggtaaccgc attgtccgca ttgcgtttgc ggcaaaatgg catcaatacg tgcaataagc
      301 gggtcgccct ctactttaaa tttgagtgcg gcataaccca gccccaagcc caataccaag
      361 gcaagaccaa gcataatgta gagcgccgtg agcatacgtt tggctagtac ctgtctaaac
      421 ccgcaaagcc cataaacgca aggctcatga gtgcggctgt taccatggcg atggctgaac
      481 cttgaaacac tttgggcaca tcagcggctt cgagtcgttc tcgcatcgac gcaaatagaa
      541 ttagcacgag cgaaaacccc agtgcgccgc ccaagccaaa taatagggat tctaaaaaac
      601 tatggcttgc ctgagcgttt aatagtggaa tgcccagtac cgcgcagtta gtggtaatga
      661 gtggcaagaa aatacctaag ccctgatata gcggcggaaa gtttttttcc atcaccattt
      721 cagtgagttg caccacactt gcaataatca ctataaatga caaggtacgt aaataaaaaa
      781 gattattggg ttcgagtaag taatgattaa ccccccaact tgtcatagag ccaatgctca
      841 acacaaacgc cgttgcccca gccatgctga gcgaagtctc taatttttta gaaactccca
      901 taaatgggca aagccccaac actttaacca gcacgatgtt gttcaccaac acggtgccaa
      961 ttaaaatcat gaagtaatgt tgtaaattga aggtcatggt tatgaattta tgcggtatct
     1021 gcgtgaaatt ataccgcgtt tgcgcctgta ctcgctaatc ttccacggta ttttatcagc
     1081 ataatatagc gtgtaaacct gatcgaggtt agtacgtttg cttatatgaa tataagcata
     1141 tcgaataaca tctacgttag attagtcgct atcatttcgt gttgtcatgg gaaatctgag
     1201 ttaaaataat ggattatcaa aatttaggtt taagtatgct gcaaggtagt ttagttgcga
     1261 ttgttacgcc gatgtttgaa gatggccgtt tagatatttc atcgctacgc gcgttgattg
     1321 attttcatct tgcagaaggc actgatggca ttgtgattgt agggacaacg ggcgagtcac
     1381 cgacagtgga ttttgatgaa cactgcttgt tgattgaaac ggctgtaagc caagtcaacg
     1441 gtcgtgtgcc ggtcattgcg ggcacggggg caaattcaac gaaagaggca attgtcctta
     1501 cccaaaaggc taaagaatta ggcgcagatg cctgcctctt ggtggctcct tactacaata
     1561 aaccaacgca agaaggtttg tatcagcact ttaaagcggt ggcggatgcg gtagaaattc
     1621 cgcaaatttt gtataacgta ccagggcgca cgggttgcga tatcagcaac gacacaacgc
     1681 tgcgcctatc ttcgcatcgc aatattgtgg gcattaaaga cgccacaggc ggcatagagc
     1741 gcggtacaga tttattatta cgtgcgccaa aagattttgt ggtgttgagc ggcgatgacg
     1801 caacagcgct aagtttgatg ttgctcggtg gcaaaggcgt gattacggtg acgggtaatg
     1861 tggcaccaaa actcatgcat gagatgtgcg aggccgccct gaaaggcgat gtggccacag
     1921 cgcgcgcgat taacgccaaa ctttttgcct tacaccaaaa actatttgta gaggctaacc
     1981 cgattcctgt gaaatgggtg ttagcacaaa tgggtttgat taaaaccggc attcgtttgc
     2041 ccatggtgca attgtcagcg cagtatcatg aagtattacg tgatgcttgc aaacccgctg
     2101 aactattatc atcaccatga gtgccatcgc caaaaaattg acagcatttt ctcaagaggt
     2161 ctgcatgaaa caattgaaca aaacgaatat gattaaacat actgccattt atgcactgct
     2221 gctttcaagc ttggcagctt gtgactccat tccatttatt gataactcgt ctgattacaa
     2281 aggcgccggc cgctctaagc cgctggaagt gccacctgat ttgacagcca gccctgtgag
     2341 cgatgcttat tctattcctg gtagcacaag ttactctact tatagtcagg cgcaagaagg
     2401 ccaagaagcg ggcgttgaaa aagtgttggc gacgcctgaa ggggtgcgtt tggtaagagc
     2461 gggcgcgcag cgctggttgg ttgtcaatgc accagcagaa aaagtttggc cagtgatacg
     2521 cgaattttgg acagaccaag gctttgccgt gcgtaaagaa gatgcgcaat tgggcgtgat
     2581 ggaaaccgaa tggattgatt ctgacgcgat taaaaaagac acatccggca atattggcga
     2641 taaatttgat aaatggttag ataaattatc aggatttgcg gataagaaaa aatttaaaac
     2701 acggctagac cacggcacag agccagacac tactgaaatt tacatgacgc atcgctcagt
     2761 tgcaggcgcg cctgatgatg gtaagaacag ggtgcaaact caactgggtg aaattgacac
     2821 gggctacaaa gccgatgcca aaaaagcacc aacggcggat gcgcgtgatg atgaacttga
     2881 tgcagagttg cttcgtcgat taatggtaaa actgggcgtt gaagagcaaa aatccaaagc
     2941 agtgatggct aacacggttg aaattaaacg tgcagaagtt gtgaaagaaa aagacggtag
     3001 cgcaacgctt tatgtgaacg acccgtttga ccgcgcatgg cgccgtgttg gcttggcgct
     3061 tgaccgcatt ggctttgtga ttgaagataa aaatcgttct gaaggcgtat tttatgtgcg
     3121 ctatgccgat gtagacattg acgacagccc taagaaaaag aaaggtttgt tagaaacctt
     3181 aaaattctgg ggagatgacg acgacaaaaa agaaccagaa accgccgcta aaaaagaaga
     3241 aaaaagcatc atggatagcc tgaaattctg gggtaaagat gacaagaaaa gcggtaacct
     3301 agaaaaacaa taccgcatta aagtggctga tggcgagtca gataaaacct cggtaacggt
     3361 ggtagataaa gacggcgcac gcgtgggcac gacaacggct aaccgtattg tggcgttaat
     3421 gtacgagcaa ttgcgctagt gactgaatat gcggtttgca tccctcggta gcggcagcgc
     3481 aggtaacgcc ctccttgttg aagtgaaccg caccacggta atgttggact gcggttttag
     3541 tatcaaagaa accgttgcaa ggcttgcgcg cttaaatgta cagcctgagg gcatttccgg
     3601 cattgtggtc acgcatgagc atgatgacca tgctggtggc gtgtttaaat ttgcggcaaa
     3661 gtatcaaatt ccggtatggc ttacttacgg cacgcacaaa atggtgtcgc gctacatgcc
     3721 gaaccagcat caactccaac tcaatattat caatagccac caagcatttg cgattgatgc
     3781 gattgaagtg cagccttacc cggtgccgca tgatgcgcgt gagccaattc aatgtttatt
     3841 ttcagatggt caaatgcaac tcggcgtgct gacggatgtt ggccgcacaa ccccacatat
     3901 tgaagaaaat ctcagaaatt gccacgcgct ggtgctggag tgcaatcatg atgccaatat
     3961 gctaaaaact ggcccctata gctggccact caaacagcgc gtgggcggtg aattag
//
LOCUS       DAOQSI010000171         3994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_24547_length_3994_cov_5.370906, whole genome shotgun sequence.
ACCESSION   DAOQSI010000171 DAOQSI010000000
VERSION     DAOQSI010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24547_length_3994_cov_5.370906"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1305)
                     /locus_tag="PKL42_09245"
     CDS             complement(<1..1305)
                     /locus_tag="PKL42_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HNU67298.1"
                     /translation="MNGFKLPAWMSMLGNRTVAAPLIIVLLLSMMILPLPALILDALF
                     SFNIALSIMVLLIGLQTKKALDFIAFPTVLLVTTMLRLSLNVASTRVVLTEGHTGPDA
                     AGKVIEAFGHFLIGGNYTVGIVVFVILTIINFTVVTKGAGRIAEVGARFTLDAMPGKQ
                     MAIDADLNAGLIGEDEARSRRAEVSQESEFYGAMDGASKYVRGDAIAGILVTVINIIG
                     GLIVGMVQHEMAFADAIKNYTLLAIGDGLVAQIPSLIISVAAGVVVSRVANKEDIGGQ
                     LVTQLFTNPNVIYVTAAIVGGMGLIPGMPNLAFLFLATLLAGLAYSVSKKQLAAEVEP
                     VQPEKTEVVTETEEASWDDVLPVDLVGLEVGYRLIPLVDKAQGGDLLKRIKGIRKKFA
                     QEVGFLAPTVHIRDNLELKPSNYRITLKGVEVASGESHFGQFL"
     gene            complement(1302..2483)
                     /gene="flhB"
                     /locus_tag="PKL42_09250"
     CDS             complement(1302..2483)
                     /gene="flhB"
                     /locus_tag="PKL42_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HNU67299.1"
                     /translation="MAEDSDLEKTEQATPKRIETAREEGDVPRSRELATVTVLFAAGL
                     SFWILGGHLNSALKTSMQQGLSFNRAEAYDPAKLLMKISEIILNLLIDFAPFALVLLI
                     VAVASPALIGGWVMSGKALAPQFNRLNPAKGLGNMFSKNSVVELVKSIAKALLVSFVA
                     YMVVSSEMEPMLLLSTFPIATGVSQTGQLLLTGFLSVVGALVIIAAIDVPFQLYQYAD
                     KLKMTKEEVRKEAKETDGNPEIKARVRQLQREMARRRMMSEVPKADVVITNPTHYAVA
                     IQYQEHGNRAPVVIAKGADATALKIREIAKEHNIVTLEAPKLARAIYAHTELNAEIPE
                     TLYTAVAEVLAYVFQLRTFNKEGGFRPDVPKALPVPDELDPHSLLPAPYTLTDEEVFA
                     S"
     gene            3168..3485
                     /gene="flhD"
                     /locus_tag="PKL42_09255"
     CDS             3168..3485
                     /gene="flhD"
                     /locus_tag="PKL42_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HNU67300.1"
                     /translation="MNEAEMMSEIRDANLNYLMLSQQMIRTDKATAIFRLGISKDIAD
                     LLEGLSNLQLLKLSSSNMMLARFRFDDSAILGMLTNYTKDSTQAHLHKAVLMSGQSAE
                     VMA"
     gene            3541..>3994
                     /gene="flhC"
                     /locus_tag="PKL42_09260"
     CDS             3541..>3994
                     /gene="flhC"
                     /locus_tag="PKL42_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HNU67301.1"
                     /translation="MTKKSIVNESEQIQLAIQMIKLGARLQLLESQTTLSRERLIKLY
                     KELKGVSPPKGMLPFSTDWFLTWQPNIHSSIFYNIYKFMITYTETSGIQAIIRAYSLY
                     REQVMTESSDPDAEPVLSLTRAWTLVRFVESKMLTMKACSKRQYWFSVW"
BASE COUNT         1189 a          874 c          814 g         1117 t
ORIGIN      
        1 taaaaactgg ccaaagtgtg attcgccgct ggcaacctcc acgcctttga gcgtaatgcg
       61 atagtttgaa ggtttcaatt ctaagttgtc gcgtatatgt acggtaggcg ctaaaaagcc
      121 aacttcttgc gcaaattttt tgcgaatgcc tttaatgcgt tttagtaaat cgccgccttg
      181 tgctttgtcc acaagcggaa tcaaacgata tcccacttct aagcctacca aatccactgg
      241 tagtacatca tcccagctgg cttcttcagt ttcagtcact acttcggttt tttcaggttg
      301 cacaggctcg acttctgctg caagttgttt tttgctcaca gagtaagcga ggcctgcgag
      361 cagggtggcc aagaacagaa aggcaagatt tggcatgcca ggaatcaaac ccataccacc
      421 gacaatagcc gccgtgacgt aaatcacatt ggggttggta aaaagttgtg tgactaattg
      481 cccgccaata tcttctttat tggccacgcg tgaaaccacc acaccggccg ccactgaaat
      541 gatgagcgaa ggaatctgtg ccaccaagcc atcgccaatc gccagtaagg tgtaattttt
      601 aatggcatcg gcaaaggcca tttcatgctg caccatgcct acgattaagc caccaataat
      661 attgatgaca gtcactaaaa tacccgcaat cgcatcacca cgcacatatt tactggcacc
      721 gtccatggcg ccatagaact ctgattcttg gcttacttca gcgcgacggc tacgtgcttc
      781 atcttcacca atcagcccgg catttaagtc agcatcaatg gccatttgtt tacctggcat
      841 ggcatctaag gtaaaacgtg cgccaacttc agcaatacgg ccagcacctt tggtaaccac
      901 ggtaaagttg atgatggtta aaatcacaaa taccacaata cccaccgtgt aattgccgcc
      961 aatcaaaaag tgcccaaacg cttcaattac tttgccagct gcgtcagggc ccgtgtggcc
     1021 ttctgtcagc actacacggg ttgaagccac gttgagtgaa agacgcaaca tggtggttac
     1081 taataacacg gttggaaagg caataaaatc aagcgccttt ttggtttgca ggccaatgag
     1141 caaaaccatg attgataacg cgatgttaaa gctaaataac gcatccagta ttaacgctgg
     1201 tagcggtaat atcatcatgg ataacagcaa tacaataata agcggtgctg caaccgtacg
     1261 gttgccaagc atgctcatcc aagcaggtag tttgaagccg ttcatgatgc aaatacctct
     1321 tcatcggtca atgtataagg tgctggtagc aaactgtgcg gatctaactc atctggcaca
     1381 ggcagtgctt ttggcacatc agggcggaag ccgccttctt tattgaatgt acgtagttga
     1441 aacacatacg ccagcacttc agccactgcg gtataaagtg tttcaggaat ttcagcattg
     1501 agttcagtat gcgcgtaaat tgcacgtgca agtttgggcg cttctaatgt tacgatattg
     1561 tgttctttgg caatttcacg aattttcaaa gcggtcgcgt cggccccttt ggcaatcacc
     1621 acaggggcgc gatttccatg ctcttgatac tgaatcgcca ctgcatagtg cgttgggttc
     1681 gtaatcacca catcggcttt cggcacttca gacatcattc tgcgccttgc catttcacgt
     1741 tgtagttgac gaacacgtgc tttaatttca gggtttccgt cagtttcttt cgcttcttta
     1801 cgcacttctt ctttggtcat ttttaattta tcggcgtatt ggtaaagctg aaaaggcaca
     1861 tcaatggcgg caataatcac caaagccccc actactgata aaaacccagt gagtaagagt
     1921 tggccagttt gcgaaacgcc tgttgctatt ggaaaagttg aaagtaaaag catcggttcc
     1981 atttcacttg aaaccaccat ataagctaca aatgatacta ataatgcttt tgcgattgat
     2041 ttaaccaact caaccactga attttttgaa aacatattgc ctaaaccttt cgcagggttt
     2101 aagcgattaa actgaggggc tagggctttg ccgctcatta cccagccgcc aatgagggca
     2161 ggggatgcaa ccgccacgat cagcagcact aaggcaaagg gtgcaaaatc aattaacaaa
     2221 ttaaggataa tctctgaaat tttcattaat aattttgccg ggtcatacgc ttctgcgcgg
     2281 ttaaaactta aaccttgttg catactcgtt tttaatgcac tgttcagatg accgcccaat
     2341 atccaaaaac tcaagccagc cgcaaaaagc acagttaccg tggccaactc gcgtgagcgt
     2401 ggcacgtcgc cttcttcccg agcggtttct atgcgcttgg gcgtggcctg ttctgtcttt
     2461 tctaaatcgc tgtcttcagc catttgtatt cagtcctgta ggtttatctt gtgccagcta
     2521 ctgcttaatc acaatggttt gctggcaagt cattacatac ttataaaatc agctttttga
     2581 tttcacaagt tttgatttca cagtgacatt attcaacagg caaacgcatt ttcatctgca
     2641 gaataaaagg gtgaaaataa gtctttcttg aattgaggtg attgaaaaat gcgatgcttg
     2701 agcttatttt taaacttttt ttaaaaataa ataacaataa aaacaattag ttataaaaaa
     2761 tatgaaaata tttttaattt tttactaaag tttttaaaag tgttgtcgta aagaatttta
     2821 agttgaaatt tattttgaat agagtgcttg tatggccggt aatgattacg gcaaaaatac
     2881 aacaatattt agaaagcgat taaattaatt cgatcagtga gcgcattaaa caacagcatt
     2941 tgccactaag gtgagcaatg taaaaattta taagtgattg aaaaatatca tattattata
     3001 gcctctgtag gtgtttgtaa gacaaacacc tacagaggcc tgtccgtttc tctcataaaa
     3061 taaaccgcca aacgctcatt gttaagcccc cgcaatcggt tcaaagtacg cctaacgcaa
     3121 cagatgtact tttaaatgaa ccgaaaacaa acgtcagggg aaataaaatg aatgaagcag
     3181 aaatgatgtc agaaattaga gatgccaatc ttaactattt aatgttatct caacaaatga
     3241 ttcgcacaga taaagcaacg gcaatttttc gtttaggcat tagcaaagat attgctgatc
     3301 tattagaagg cttaagcaat ttgcaattac ttaaattaag tagcagtaac atgatgttag
     3361 ctagattccg ttttgatgac agcgccattt taggcatgct caccaactac accaaagaca
     3421 gcacacaagc gcacttacac aaagcggtgt taatgtctgg ccaatcagca gaagtaatgg
     3481 cgtaagttat ccaagtgatt ttttaaatca agattaaaca catcaggtag gggtagcatc
     3541 atgactaaaa aaagtattgt gaatgaatca gagcaaatcc agttggccat tcaaatgatt
     3601 aagctgggcg cacgattaca acttttagag tcgcaaacca cgctatctcg cgaacgttta
     3661 attaagcttt ataaagaatt aaaaggcgtt tcgccaccca aaggtatgtt gccgttttca
     3721 accgattggt ttttaacttg gcagccaaat attcactcat ccatttttta taacatctac
     3781 aagtttatga ttacgtatac cgaaacttcc ggtattcaag ccatcattcg tgcttatagt
     3841 ttgtaccgcg aacaggtgat gacagaaagt tcagacccag acgctgaacc agtattgtct
     3901 cttacccgtg cctggacact ggtacgtttt gttgaaagca aaatgctaac catgaaagcc
     3961 tgcagtaaga gacaatactg gttcagcgtc tggg
//
LOCUS       DAOQSI010000172         3985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_24620_length_3985_cov_4.680407, whole genome shotgun sequence.
ACCESSION   DAOQSI010000172 DAOQSI010000000
VERSION     DAOQSI010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24620_length_3985_cov_4.680407"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /gene="phoU"
                     /locus_tag="PKL42_09265"
     CDS             complement(<1..604)
                     /gene="phoU"
                     /locus_tag="PKL42_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNU67302.1"
                     /translation="MSSEHIFKQYDAELESVRAKVLEMGGLVEKQIADAMKALVESNP
                     TLAQTVVKKDHQVNALEVQIDEDCSTIIARRQPTAGDLRMILMMIKTITDLERIGDEA
                     SKIARFAISIHEANRAWSTKFSDVKAMASVTQTMLRMALDAFARSDETKVQEIAQMDE
                     KVDEQYQAAIRQMITFMLEDPRTISMTLEVLFVAKAIERIG"
     gene            complement(644..1423)
                     /gene="pstB"
                     /locus_tag="PKL42_09270"
     CDS             complement(644..1423)
                     /gene="pstB"
                     /locus_tag="PKL42_09270"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNU67303.1"
                     /translation="MVNITTPIKAEARDLNFFYSNGNQALKSINMPLYEKKITALIGP
                     SGCGKSTFLRCFNRMHDLYPGNKYQGQIVMHPDNTNILEAGVDPIEVRMRISMVFQKP
                     NPFPKTIYENVAYGLRVRDEKNKRTIDDKVEEALKGAAFWNEVKDRLQDLAFNLSGGQ
                     QQRLCIARALATDPEIMLFDEPTSALDPIATANIEELMSELKEKLTILVVTHNMQQAA
                     RVSDYTAYMYLGELMEYGDTNQIFTRPTRKETEDYITGKFG"
     gene            complement(1454..2398)
                     /gene="pstA"
                     /locus_tag="PKL42_09275"
     CDS             complement(1454..2398)
                     /gene="pstA"
                     /locus_tag="PKL42_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNU67304.1"
                     /translation="MTTEMKASVLKNLDAVRAMIRRHKRNDMLFAMVGLLALMLGLLT
                     LLTLFIDLVMDGYPRFNLQFFSDFPSRRAGSAGLLSAWVGSALVMLVTFLTAVPVGVA
                     AGLYLEEYAPKNWFSDLIEINVSNLAGVPSIIYGLLALGLFVYGFNFGQSILTAGLTL
                     GLLILPIVIVSTREAIRGIPVAIREAAYAVGATKWQVVSDHVVKYSQGGILTGVIIGM
                     SRAIGETAPIITIGALTFIAFLPPAPVSDVAPFINFQWLDSGFTVLPIQMFNWLSRPD
                     HAFHINAAATGAIIIVVTLLMNGTAIYLRYKIRKNIKW"
     gene            complement(2398..3342)
                     /gene="pstC"
                     /locus_tag="PKL42_09280"
     CDS             complement(2398..3342)
                     /gene="pstC"
                     /locus_tag="PKL42_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNU67305.1"
                     /translation="MNTATMTENTTTLTISPRLAKSVRRNIKERVIELILMLAALSAV
                     FTTFAIVAILFYESFGFFKTVSLVEFFTGTEWTPLFEDAHYGILPLIAGTLTTSLVAL
                     AVAVPIGTIAAIYLSEFASHKTREITKPILELLVGVPTIAFGYFALLFVTPLLQKVYP
                     DLPGFNMLSAGLVMGVMIIPYIASVAEDAMRAVPMSMREGSYAMGATRFQTAIRVITP
                     AATSGIVAAYILAISRAVGETMVVAVAAGQQPNFTFNPTEGAATITAYIVQVAMGDLP
                     HASIGYQSIFAAGLVLFAMTLGFNLIGHVVRKKFREQY"
     gene            complement(3456..>3985)
                     /locus_tag="PKL42_09285"
     CDS             complement(3456..>3985)
                     /locus_tag="PKL42_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNU67306.1"
                     /translation="ALFGPGTASGTFDYFTEAINGKSKASRTDYTPSEDDNVLVQGVS
                     GNTGGLAYFGYAYYEENKDKLRAIPISAKAGSPAVMPSPEAVKDGTYQPLARPIFIYV
                     NATSAAFKPEVKAFVNYYLEHAPTLVKEVKYVPLTTEDYAAVKEHFKALKPGTGFGGK
                     NEVGIKVKDLINRIK"
BASE COUNT         1108 a          927 c          855 g         1095 t
ORIGIN      
        1 cgccaatgcg ctcaatggct tttgcaacaa acaatacttc aagcgtcata gaaatggtgc
       61 gtggatcttc cagcatgaac gtaatcatct gacgaatggc agcttgatat tgctcatcta
      121 ctttttcatc catctgggcg atttcttgca ccttggtttc gtctgagcgt gcaaacgcat
      181 cgagtgccat acgcagcatg gtttgcgtga cggaagccat agcttttaca tcagaaaact
      241 tagttgacca cgcgcggttt gcttcatgaa tactaatcgc aaaacgtgca atttttgaag
      301 cctcatcacc aatacgctca aggtcggtaa tggttttaat catcatgaga atcatgcgca
      361 aatccccagc agtaggctgg cggcgcgcga tgatagtgct gcaatcttca tcaatctgca
      421 cttcaagcgc attcacttga tggtcttttt tcaccactgt ttgggccagt gttgggtttg
      481 attccaccag cgctttcatt gcatcggcaa tttgtttttc aaccaagccg cccatttcga
      541 gcactttggc gcgcacgctt tctaactcgg catcgtattg cttaaatata tgttctgaac
      601 tcatggtgtt tccttaaata atcttcaaaa ttttgcagtt aaattaacca aacttaccag
      661 tgatgtaatc ttcggtttct ttgcgtgttg gacgggtgaa aatttggttg gtgtcgccat
      721 attccatcaa ctcacccaaa tacatgtagg cggtgtagtc tgaaacgcgc gcggcttgct
      781 gcatattgtg cgtcaccacc aatatagtga gtttttcttt gagctcactc attaattctt
      841 caatattggc tgtggctatt gggtctagcg cagaggttgg ctcatcaaac agcataattt
      901 cagggtcagt cgccaaagcg cgggcaatac acaagcgctg ttgctggccg cccgatagat
      961 taaacgctaa atcttgcaaa cggtctttga cttcattcca aaatgctgcg cctttaaggg
     1021 cctcttccac tttgtcatca atggtgcgtt tgtttttttc atcgcgcaca cgtaagccat
     1081 aagccacatt ttcataaatg gttttgggaa atgggtttgg cttttgaaac accatactga
     1141 tgcgcatgcg tacttcaatc gggtcaacgc ctgcttctag aatattcgtg ttatccggat
     1201 gcatcacaat ctggccttgg tatttattgc caggatataa atcatgcata cggttaaagc
     1261 agcgtaaaaa agtagatttt ccgcaacccg atgggccgat gagcgcggtg atttttttct
     1321 cataaagcgg catgttgatg ctttttaatg cctgattacc attgctgtaa aagaagttta
     1381 aatcgcgtgc ttctgctttg attggggttg ttatgtttac catgtaagtg attccctaat
     1441 tatgcctaaa tttttaccat ttaatgtttt tacgaatttt gtagcgcaag taaattgcag
     1501 tgccgttcat gagtaatgtc accacaataa taatggcgcc cgtggccgcg gcattgatat
     1561 gaaacgcgtg atctggacgt gatagccagt taaacatttg aatcggcaac accgtaaacc
     1621 cagaatctaa ccattgaaaa ttgataaatg gcgctacgtc tgacactggt gctggaggta
     1681 agaacgcaat aaatgtgagt gcgccaatgg tgataatcgg tgcagtctcg ccaattgcgc
     1741 gcgacatgcc aataatcaca ccggtgagta tgccgccttg tgaatatttc accacatggt
     1801 cacttaccac ttgccattta gtagcaccaa ccgcataggc tgcctctcga atcgccaccg
     1861 gaataccgcg aatcgcttca cgtgtagaca caatcacaat cggtaatatc agcaaaccaa
     1921 gtgttaagcc tgcggttaaa atgctttgac caaaattaaa gccgtagaca aacagaccca
     1981 gtgccagcaa accgtaaata atcgaaggca cacccgcaag atttgaaacg ttaatttcaa
     2041 tcaggtctga aaaccagttt tttggtgcgt attcttctaa gtataaacct gccgcaacac
     2101 caactggcac agccgttaaa aaagtcacca acatcactaa cgctgaccct acccaagcgg
     2161 agagcaggcc ggcactgccg gcgcggcgcg atggaaaatc gctaaaaaac tgtaagttaa
     2221 agcgaggata gccatccatc accaaatcga taaaaagtgt tagcaatgtc agcaggccga
     2281 gcatgagcgc cagcaagcca accattgcaa acaacatatc attgcgttta tggcggcgaa
     2341 tcattgcccg cactgcatcc aagtttttaa ggactgaagc cttcatttcc gttgtcatta
     2401 gtattgctcc ctaaatttct tacgcaccac atgaccaatc aagttaaacc ctaaggtcat
     2461 cgcaaacagc accaaaccag cagcaaaaat actttggtag ccaatgctgg catgaggtaa
     2521 atcacccatg gccacttgca caatataggc cgtaatggtt gcggcgcctt cggtaggatt
     2581 aaaagtgaag ttgggctgct ggccagccgc aacggccacc accatagttt cacccactgc
     2641 gcgagaaatc gccaaaatat aagcggccac aatgcctgaa gtggctgcag gtgtgatgac
     2701 ccttatcgcg gtttgaaaac gcgtcgcgcc catggcgtac gagccttcac gcatactcat
     2761 aggcacggca cgcatggcgt cttctgccac tgaagcaata tagggaataa tcatcacgcc
     2821 catcaccaag cctgcgctca acatattaaa gccgggtaaa tctgggtaaa ctttttgtag
     2881 taatggcgtc acaaataaca aggcaaagta accaaacgca atggtaggca cgcccactaa
     2941 tagctctaaa attggcttgg taatttcgcg ggttttgtgt gaggcaaatt cagaaagata
     3001 aatggccgca atggtgccga ttggcaccgc caccgctaat gccacgagcg aagttgtgag
     3061 cgtacctgca attaggggca aaatgccgta atgggcatct tcaaaaagtg gtgtccattc
     3121 agtgcccgta aaaaattcaa ctaacgaaac ggttttaaaa aaaccaaagg attcataaaa
     3181 caatatggcc acaatggcaa aagtagtaaa tacagcggag agcgcagcga gcattaatat
     3241 cagctcaatc acgcgctctt tgatatttct acggacactt ttggccaaac gcgggcttat
     3301 ggttaaggtg gtggtatttt cagtcatcgt ggcagtattc atatgaaggc ttttttaaac
     3361 atcttgttta aaaaaacttg gtcactcccg cttgcgcgag aatgactaag ttgatggggt
     3421 tttgacaaaa tcaattaaaa acttaacaat tcaagttact taattctgtt aattaaatct
     3481 tttactttaa tccccacttc attttttcca ccaaagcctg tgccaggttt taaagctttg
     3541 aagtgctctt ttaccgcagc gtaatcttct gttgttaatg gcacatattt cacctctttc
     3601 accaaagttg gtgcgtgttc caagtagtag tttacaaaag cctttacttc tggcttaaac
     3661 gctgccgatg ttgcattaac ataaataaag attgggcgtg ctagcggctg ataagtgccg
     3721 tctttcactg cttcaggtga aggcattact gctggtgaac ctgcttttgc tgagatagga
     3781 atggctctta atttatcttt attttcttca taataagcat aaccaaagta ggccaaacca
     3841 cccgtattac cgctcacgcc ttgtaccaac acgttatcat cttctgatgg cgtgtaatct
     3901 gtacgactcg cttttgattt accattgatg gcttctgtaa agtaatcaaa cgtacctgaa
     3961 gcggtacctg ggccaaataa agcca
//
LOCUS       DAOQSI010000173         3928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_25161_length_3928_cov_4.824684, whole genome shotgun sequence.
ACCESSION   DAOQSI010000173 DAOQSI010000000
VERSION     DAOQSI010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25161_length_3928_cov_4.824684"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="PKL42_09290"
     CDS             <1..907
                     /locus_tag="PKL42_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNU67307.1"
                     /translation="SGVDHIRHPLDIAKNAFTKGVPQDGGIALANEFEQRILTLHDPA
                     TIAALIVEPMQGSAGVIVPPTAYLKRLREICTKYGILLIFDEVITGFGRLGTKFGADY
                     FDVIPDMLTCAKGLTNGVVPMGAVAVKREIYDAFMENTPTGAIELPHGYTYSAIPIAC
                     AAAIATLNIFQQEKLEDRAASLSPHLEAAVHHLKGLPMVKDIRNIGLVAGIEFEAITG
                     KMGAFAFAVYLKCFELGLHVRYTGDIIAISPPLTIEKHEIDRIFNILSEAIRFVASQG
                     VAGLDANNYLKNEQTWALHQQTTNA"
     gene            996..2492
                     /locus_tag="PKL42_09295"
     CDS             996..2492
                     /locus_tag="PKL42_09295"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HNU67308.1"
                     /translation="MNTIGHYVNGQHLTGTSNRFSDVYNPATGEVSAKLCNATATETE
                     AAIAAALAAFPAWAATPALRRARIMFKFKALLDEHANDLAKAISLEHGKTISDARGEV
                     TRGIEVVEFACGMPHLLKGDYNEQVGTGIDTYSMHQPLGVVAGITPFNFPVMVPLWMI
                     PMALATGNTFVLKPSEKTPSASLMLADLLAEAGLPAGVFNIINGDKEAVDVLLTDKRV
                     QAISFVGSTPIAEHIYSTGTKNGKRVQALGGAKNHLVVMPDADLDQVADALIGAGYGS
                     AGERCMAISVAVTVGDIADQLVEKLKEKVSTIVIDQGLNEKADMGPLVTPQHFAKVRE
                     YVDLGIAEGAKLVIDGRDFTKQGFEKGFFLAPCLFDHVSKNMRIYKEEIFGPVLAIVR
                     AKTFEEALELVNEHEFGNGTAIFTRDGEAAREFTSRVQAGMVGVNVAIPVPIAFHSFG
                     GWKRSLFGDHHIYGTEGIRFYTRLKTTTSRWPSPSKNSGAEFVMPTMK"
     gene            complement(2710..>3928)
                     /gene="ccmI"
                     /locus_tag="PKL42_09300"
     CDS             complement(2710..>3928)
                     /gene="ccmI"
                     /locus_tag="PKL42_09300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HNU67309.1"
                     /translation="MWGFLTAALLLIGMVLVILIPALLRPQSTLNRDDNAQRREIFRA
                     QFAEIEQDLASGVLDAAQYELAKTELERRMLDEVTSSPLAATVAKPDRRLAVILVFLV
                     PLTAFWLYVKIGNPEAIVNPTGAAMMDGKPMTQADVEGLLASLAAKLEKNPDDGAGWV
                     LLGNSYVKIGKYAEAVKAYEQATKKITDNAQLYADYADALAATNDYKLEGLPVELANQ
                     ALKIDARNIKAKMLLATAAYDRQDYKEAIALWEKIITELPKEAEIIPDIKAAIAKAQS
                     LAGETKSTSVQKVAVNAGIRGTVRLSPALTKALDANATLFVFARAAQGAPMPIAIVRA
                     SAKDLPYQFQLDDTSAIMPGNKLSQADAVVVVARISKSGDAKPISGDLQGMSAVMKPV
                     GENVEIEINEVLK"
BASE COUNT         1024 a          981 c          916 g         1007 t
ORIGIN      
        1 aagcggtgtt gaccatattc gccacccgct cgacattgcc aaaaatgcgt ttaccaaagg
       61 tgtgccgcaa gacggtggca ttgcgctggc caatgaattt gagcagcgca ttttgacctt
      121 acatgaccca gccaccatcg ccgcgctgat tgttgaacca atgcaaggtt cggcaggcgt
      181 gattgtgccg ccgacagctt atttaaaacg cctgcgcgaa atttgtacca aatacggcat
      241 tttgctgata tttgatgaag tcattaccgg ttttggcagg ctaggcacaa aatttggcgc
      301 ggactatttt gatgtgattc ctgatatgct cacctgcgca aaaggcctta ccaatggtgt
      361 ggtgccgatg ggtgcagtgg ctgttaagcg cgagatttat gatgcattta tggaaaacac
      421 gccaactggc gcaattgaat tgccgcatgg ttacacgtat agcgccattc ccattgcctg
      481 cgcggcagcc attgccacct taaatatttt tcaacaagaa aaattagaag accgcgcggc
      541 cagtttaagc ccacacctcg aagccgctgt gcatcaccta aaaggcctgc ccatggtaaa
      601 agacattcgc aacattggtt tagtggcagg cattgagttt gaggcaatta ccggcaaaat
      661 gggcgcgttt gcctttgcgg tttatctcaa atgttttgag ctaggtctgc atgtacgcta
      721 caccggcgat attattgcaa tatcgcctcc gctcaccatc gaaaaacacg aaattgatcg
      781 tatttttaac atactttcag aagccattcg atttgtagca agccaaggtg tggcgggatt
      841 ggatgcaaat aattacctta aaaatgagca gacttgggca ttgcatcaac aaacaaccaa
      901 tgcttaacac ctttatgtag gcgcgcaata tatcgctaac ctactataac cctatgaaat
      961 taaaaaacaa tgcgtaaaat tacggagcat tcaacatgaa cacaatcgga cactacgtca
     1021 atggtcagca ccttacaggc actagcaaca gattcagtga cgtttacaac ccagccacag
     1081 gtgaagtctc tgccaaacta tgtaatgcta cggccactga aactgaagca gcaatcgccg
     1141 cagcacttgc tgccttcccc gcttgggcag ccacgcccgc cttacgccgt gcgcgcatta
     1201 tgtttaaatt taaagcctta ctcgatgaac acgccaatga tttagccaaa gccatttcac
     1261 tcgagcacgg caaaactatc tccgatgcac gcggtgaagt tacacgcggc attgaagtgg
     1321 ttgagttcgc ttgtggtatg ccgcatttac ttaaaggtga ctataacgaa caagtcggca
     1381 caggcattga cacatacagc atgcatcagc cgcttggcgt agtggctggc atcacgccct
     1441 ttaattttcc ggtcatggta ccgctatgga tgattccgat ggccctggct accggcaaca
     1501 cctttgtgct gaaaccttct gaaaaaacac caagcgccag cctaatgttg gccgatttac
     1561 tggcagaagc tggcttgcct gctggcgtat tcaacatcat caatggcgat aaagaagccg
     1621 tggatgtgct gctaaccgat aaacgcgtac aagccatcag ctttgttggc tcaacgccaa
     1681 tcgccgaaca catttatagc actggcacta aaaatggcaa acgcgtacaa gcgctgggtg
     1741 gcgccaaaaa ccacttagtg gtgatgcccg atgcagattt agaccaagtc gccgatgcgc
     1801 taattggcgc aggctacggc tctgcgggcg agcgctgcat ggccatttct gtagccgtca
     1861 ccgtgggcga tattgctgac caactcgttg aaaaactcaa agaaaaagtc agcaccatcg
     1921 tcattgacca aggtttaaat gaaaaagcag acatgggccc gctagtaaca ccgcaacact
     1981 ttgctaaagt acgcgaatat gttgatttag gcattgctga aggtgcaaaa ctggtgattg
     2041 atggccgcga tttcaccaaa caaggctttg aaaaaggctt ctttttagca ccctgcttgt
     2101 ttgaccatgt cagcaaaaat atgcgcatct ataaagaaga aatttttggc ccggtgttag
     2161 caatcgtgcg ggcaaaaacc tttgaagagg cacttgagtt ggtaaacgaa cacgaatttg
     2221 gcaacggcac agcgatattc acccgcgatg gcgaagccgc gcgcgaattc actagccgcg
     2281 tgcaagcggg catggtgggc gtcaatgtgg ccattccggt gccgattgcc ttccacagct
     2341 ttggcggctg gaaacgctct ctctttggtg accaccacat ttatggcacg gaaggcattc
     2401 gtttttacac gcgcttaaaa accaccacct cgcgttggcc ctcgccaagc aaaaatagcg
     2461 gtgctgaatt tgtgatgccg accatgaagt aaggttgcat gaatagaaat tgggctgggg
     2521 aaattggcag attgttaggc gtaaaactgg ggtttaaaag cgtataaaac tgctatttaa
     2581 acacacaacc tttttcgatg ctctacgagc ctttatttac aaggcttcca gagcatattt
     2641 tttctctcga gtgaatttag tgactttact acttgcatca ttgcgcaatt aatcatgcgc
     2701 gcctgatggt tacttcaaca cttcgttgat ttcaatttcc acattttcac caaccggttt
     2761 catcacggca gacatgccct gcaaatcgcc agatatcggt ttggcatcgc cactttttga
     2821 aatgcgcgcc actactacca ctgcatccgc ttgtgagagc ttattgccgg gcataatcgc
     2881 ggatgtgtca tctaactgaa actgataagg caaatctttt gcactagcgc gcacaatcgc
     2941 aattggcatg ggtgcgccct gcgccgcacg ggcaaataca aatagggtag cattggcatc
     3001 taacgctttg gtcaaggcgg gagacaaacg cacagtgcca cgaataccag cattcactgc
     3061 cactttttgt actgaagtgg attttgtttc accagcaagc gattgcgctt tggcgattgc
     3121 cgctttgata tctgggataa tttcggcctc tttaggtaac tctgtgataa ttttctccca
     3181 cagcgcaatg gcctctttat aatcttgacg atcatacgcg gcggtggcca gcaacatctt
     3241 tgctttaatg ttgcgtgcat ctatttttag ggcttggttg gccaactcaa caggcaagcc
     3301 ttctaactta tagtcattgg ttgccgccag tgcatctgca taatcagcat agagttgtgc
     3361 gttatcggtg atttttttgg tggcttgttc gtaggctttt acggcctccg catatttgcc
     3421 aattttgaca tatgaattcc ccaataaaac ccagccggcg ccatcatcag gatttttttc
     3481 aagctttgcc gctaatgatg ccagcaaacc ttctacatcg gcctgcgtca tcggcttgcc
     3541 atccatcatg gcagcccccg ttgggttcac aatggcttct gggttgccaa ttttaacgta
     3601 cagccaaaaa gctgtcagtg gcaccaaaaa aaccagtatt acagcgagtc ggcggtctgg
     3661 tttcgcaacc gtggctgcga gcgggctact tgtgacttca tctagcattc gccgttctag
     3721 ctcggttttg gcaagctcat actgcgctgc atctaacacg ccgctggcta aatcctgttc
     3781 aatctcggca aactgcgcgc gaaaaatctc gcgcctttgt gcgttatcat cgcgattcaa
     3841 agtcgattgc ggacgcaata atgctggaat gagtatgacc aacaccatgc caatcaacaa
     3901 aagtgctgct gttagaaaac cccacatt
//
LOCUS       DAOQSI010000174         3901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_25435_length_3901_cov_4.248310, whole genome shotgun sequence.
ACCESSION   DAOQSI010000174 DAOQSI010000000
VERSION     DAOQSI010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25435_length_3901_cov_4.248310"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PKL42_09305"
     CDS             complement(<1..466)
                     /locus_tag="PKL42_09305"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168441.1"
                     /note="catalyzes the formation of 2-C-methyl-D-erythritol
                     4-phosphate from 1-deoxy-D-xylulose-5-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNU67310.1"
                     /translation="MDNSSTSHQRQQVTILGATGTIGLQTLDVIAQHPDRFEVFAITA
                     NSNVKGLLSLCQQYSPKFAVMLQEKACEELSLQLKAIGSSTVVLCGGDALNHVSSHED
                     VDVVMAAIVGAAGLHPAMAAAKAGKRILLANKETLVMAGDLFMQAVVEGGATL"
     gene            complement(459..1295)
                     /locus_tag="PKL42_09310"
     CDS             complement(459..1295)
                     /locus_tag="PKL42_09310"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNU67311.1"
                     /translation="MLKTRIITAILLVIGFLMALFFTSSFAWSLITLFITVIAVWEWM
                     KLIKLNAKQITISLTGTLIIGLMLVAFSKFSSETILELYADKLVLLLLAVSAVFWVVV
                     APTWLITRKKINQKLFMSTLGISLLLATWIALIGLHKISPLLLLSVLATVWIADSAAY
                     FAGKKFGKHKLAPEISPGKTWEGVMGALFAVTLYGLLLCHFQHLSRWLILGLWLIVIL
                     SVMGDLFESLLKRQSNVKDSSQLLPGHGGVLDRIDGLIPTLPLVLLSIYFPLFTNLQL
                     HG"
     gene            complement(1348..2106)
                     /locus_tag="PKL42_09315"
     CDS             complement(1348..2106)
                     /locus_tag="PKL42_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNU67312.1"
                     /translation="MAFFSSATQSIPDVAAIPKHIAVIMDGNGRWARKRFLPRVAGHK
                     RGVETVRDLVKYCLTLKVEHLTLFAFSSENWRRPSEEVSFLMGLFMQALKREVDKLHK
                     NNIKLIMIGDRSRFDAALIEQINAAETLTQHNTALVLTIAANYGGRWDILQAVNKMQA
                     ATPELSGHYNEAHLTPYLSMHYAPEPDLFIRTGGEKRISNFLLWQLAYTELYFTDTLW
                     PDFNEEAFKLAIQSYQNRERRFGRTSEQLAGPAQ"
     gene            complement(2161..2721)
                     /gene="frr"
                     /locus_tag="PKL42_09320"
     CDS             complement(2161..2721)
                     /gene="frr"
                     /locus_tag="PKL42_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNU67313.1"
                     /translation="MMISDVKKTADQKMLKSLEVLKADLAKVRTGRAHVGLLDHVMVE
                     YYGSMVAVNQVANVNLGDARTIHVQPFEKTMIAKVEKAIRDCDLGLNPATNGDVIRVP
                     MPMLTEERRREMTKIVRSEAEGAKVSIRNVRRDANDALKKLLKDKEISEDDERRAQDD
                     IQKSTDKAVAEVEKLLQVKEAELMAV"
     gene            complement(2814..3530)
                     /gene="pyrH"
                     /locus_tag="PKL42_09325"
     CDS             complement(2814..3530)
                     /gene="pyrH"
                     /locus_tag="PKL42_09325"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNU67314.1"
                     /translation="MSKPAYKRILLKLSGEALMGDDAYGINRATISRIVAEVKEVVDL
                     GVQVAVVIGGGNIFRGVAPAAEGMDRATADYMGMLATVMNAMALQDAMKNIGLNARVQ
                     SALNIEQVAEPYIRGKAIRYLEEGRVVIFGAGTGNPFFTTDTAAALRGMEMNAEIVIK
                     ATKVDGIYTEDPKSNPDAMRYKTISFDEAISKNLKVMDATALTLCRDQKLPISVFSIF
                     KQGALKKVIMGEDEGTMVTP"
     gene            complement(3730..>3901)
                     /locus_tag="PKL42_09330"
     CDS             complement(3730..>3901)
                     /locus_tag="PKL42_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HNU67315.1"
                     /translation="VTLLSQTFVKDDKLTIEQLLKSKGASVASFTMFTVGEGIEKAVV
                     DYAAEVAAAAKV"
BASE COUNT         1110 a          904 c          743 g         1144 t
ORIGIN      
        1 gtaaagttgc cccaccctcc actacagctt gcataaataa gtcacctgcc atcaccagcg
       61 tttctttatt ggcaagcaag atacgttttc ctgccttggc agcggccatg gcagggtgca
      121 aacctgctgc tcccacaatt gccgccatca cgacatcaac gtcctcatgt gaagacacgt
      181 gattcaatgc atcaccacca cacaatacca cggtgcttga acctatcgcc tttaattgca
      241 gacttaattc ttcacatgcc ttttcttgta gcatcaccgc aaattttggt gaatattgct
      301 gacataacga taataaacct ttaacgttac tatttgccgt gatggcaaac acttcaaaac
      361 ggtctgggtg ttgtgcaata acgtctagcg tttgtaagcc aattgtgccc gttgcaccga
      421 gtatcgtgac ctgctggcgt tgatgagaag tcgaactgtt atccatgcaa ttgtagattc
      481 gtgaaaagtg ggaaatatat gctaagcaac actaatggca aagttggaat taaaccatcg
      541 attctatcga gcacaccgcc atggcctggc aacaattgac tactgtcttt cacattagac
      601 tgtcgtttta acagcgactc gaacaaatca cccattacac ttaagataac gatgagccat
      661 agtcctaaaa tcaaccaacg actgagatgt tgaaaatggc ataaaagtaa gccatacaac
      721 gtaacggcaa ataatgcccc catgacgcct tcccatgttt ttcctgggct tatttcaggt
      781 gctaacttgt gcttgccaaa tttctttcct gcaaaatagg ccgcactatc ggcaatccaa
      841 accgttgcta aaacagaaag taacaacaat gggctaattt tatgcaatcc aatcaaagca
      901 atccaagtag ctaacaaaag tgaaataccc aaagtcgaca tgaataattt ttgattgatt
      961 ttttttctgg taataagcca agtaggagca acaacaaccc aaaatacagc tgacacggct
     1021 aaaagtaaca ataccaactt atcagcatac aactctagta tggtctctga tgaaaactta
     1081 gagaaagcaa ccaacatcaa tccgataatt aaagtgcctg ttaggctaat ggtaatttgt
     1141 ttcgcattaa gtttaatgag tttcatccat tcccaaaccg ctataaccgt gataaataag
     1201 gtgatgagtg accaggcaaa tgatgaggta aagaatagcg ccatcaaaaa cccgatgact
     1261 aacaaaatag ctgtgataat tctggtttta agcatgagga ggcacgaact tgttggaatt
     1321 gttgtgggtc attattggaa tgagtaatta ttgagcagga ccagcgagtt gctcactcgt
     1381 tctgccaaag cgccgctcac gattttggta ggactgaatc gccagtttga atgcttcttc
     1441 attaaaatct ggccacagcg tatctgtaaa atacagttcg gtataagcta attgccatag
     1501 taaaaaattg ctaatacgct tttcaccgcc agtgcgtata aataaatcag gctcgggcgc
     1561 ataatgcatc gataaatacg gggttaaatg cgcttcgttg taatggccag acaattcagg
     1621 tgttgccgct tgcattttat taacagcctg caaaatatcc cagcgcccgc cgtagttggc
     1681 agcaatcgtg agtaccagtg cggtattgtg ctgtgtgaga gtttctgctg cattaatttg
     1741 ctcaatgagt gccgcatcaa agcgactgcg atcgccaatc ataatcagtt tgatattgtt
     1801 tttatgcagc ttatctactt cacgctttag cgcttgcata aatagaccca ttaaaaatga
     1861 aacctcttcg ctaggtctgc gccagttttc gctactaaaa gcaaacaaag ttaagtgttc
     1921 tacctttaac gttaagcaat atttaacaag gtcacgtaca gtctcaaccc cacgcttatg
     1981 cccggcaact ctaggtaaaa aacgcttacg tgcccaacgt ccattaccat ccataatcac
     2041 cgcaatgtgt ttaggaattg ctgcgacatc tggaatgctt tgggttgcgc tactaaaaaa
     2101 tgccattcag gacccagtta aataatgtgg aaagtgtcaa ttgcgtaggc tatagtgaac
     2161 ttaaaccgcc atcaattcag cttcttttac ctgtagaagt ttttcaactt cagcaacggc
     2221 tttatccgtt gatttttgaa tatcatcttg tgcacggcgc tcgtcatctt ctgatatttc
     2281 tttatctttt aagagctttt taagcgcatc attggcatca cggcggacat tgcgaatgga
     2341 tactttggca ccctctgcct ctgagcgtac tattttggtc atttcgcggc gacgttcctc
     2401 tgtcagcatg ggcataggaa cacgaatcac atcgccattt gtggctggat ttaaacctaa
     2461 atcacagtct cgaatggctt tttcaacttt ggcaatcatg gttttttcaa agggttgtac
     2521 atgaattgta cgggcatcgc ccagattcac attggccact tgattgaccg caaccataga
     2581 accataatac tcaaccatta catgatcaag caagcctaca tgcgcacgtc ctgtgcgtac
     2641 ttttgccaaa tcagccttga gtacctctaa agactttaac attttttgat cggcagtttt
     2701 tttaacatca ctaatcatca acttctccaa ctattatttc aatatgttct tattttatgt
     2761 ttttgaaact taggaactca ctgacgcagt ttcacctaag caatccaacg ccattaaggc
     2821 gtaaccattg tgccttcatc ttcacccatg atcacttttt tcaacgcacc ttgcttaaaa
     2881 atactgaaaa ccgaaatagg caacttttga tcacgacata aagtcagcgc tgttgcatcc
     2941 atcactttga gatttttgct aatcgcctca tcaaagctga ttgttttgta gcgcattgca
     3001 tccggattgc tttttgggtc ttctgtataa atgccatcta ctttggttgc tttaatcacg
     3061 atttcagcgt tcatttccat tccgcgcagt gcagcagccg tatctgtggt gaaaaacggg
     3121 ttacctgtcc ccgcaccaaa aatgaccacg cgcccttctt ctaagtagcg aatcgctttg
     3181 ccacggatat aaggttcagc cacttgctcg atattcaatg cgctttgtac acgcgcattt
     3241 aaaccaatgt ttttcattgc atcctgtagg gccatggcat tcataacggt agcaagcatg
     3301 cccatgtagt cagcagtggc tctgtccatt ccttctgcgg caggtgctac gccacgaaag
     3361 atattgccac cgccgatgac cactgcaact tgtacgccta aatcgaccac ttctttgact
     3421 tctgcgacaa ttctgctgat tgtagcgcgg ttaatgccat aagcgtcatc gcccatgagc
     3481 gcctcaccag aaagtttaag tagtatgcgt ttgtaggcag gtttagacac aaattttcct
     3541 ctttataaag tttgcttatt taagcataaa ttcgcttttt taggcatcac ttaactgctt
     3601 aaaaattgaa taaaatgata atacgttatg ttttgttaac agtatttaat taaaaaaaat
     3661 ggattaagca attcgcttaa tccatttatt agcctaagaa ggctgccaat cttttgattg
     3721 gctatccaat tacactttag ccgctgcagc tacttcagct gcataatcta ccactgcctt
     3781 ttcaatccct tcacctacgg tgaacatggt gaatgaagca actgaggcgc ctttagactt
     3841 caacaattgc tcgatggtga gtttgtcatc tttcacaaaa gtttggctga gcaaggtcac
     3901 c
//
LOCUS       DAOQSI010000175         3874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_25687_length_3874_cov_5.139042, whole genome shotgun sequence.
ACCESSION   DAOQSI010000175 DAOQSI010000000
VERSION     DAOQSI010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25687_length_3874_cov_5.139042"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="PKL42_09335"
     CDS             <1..698
                     /locus_tag="PKL42_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNU67316.1"
                     /translation="AYLLTDPDRKITVQAQKRLDAIQLMEDLGAGFHLAMHDLEIRGA
                     GELLGDSQSGEMQEIGFHLYSDMLNHAVKQLKAGKEPDLNAPLGVTTEINLHTPALLP
                     TKYCPDVHERLVVYKRLANCVDDDELDNLQEELIDRFGLLPEQGEALMACHRLRIASK
                     AIGVIKIDASDAAIQLQFIPKADIDPMKLINLLQRDKRCRMNGPDKLRITVSFPTIHL
                     RADFVKSILREFI"
     gene            complement(717..1292)
                     /locus_tag="PKL42_09340"
     CDS             complement(717..1292)
                     /locus_tag="PKL42_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HNU67317.1"
                     /translation="MKIFSGLFQRAMIWARSPNAPWYLGALSFAESSFFPIPPDVMLA
                     PMSLANPNKAWRFALITTVTSVLGGIFGYFIGITMFDVIEPHLKASHYWEAYLTAVTW
                     FKDYGFWAIFIAGFSPIPYKVFTIAAGTLSMALIPFTLASLIGRGMRFFMVAGLMKWG
                     GPSMEAKLHQYVDRIGWATVAIVAALFLIYR"
     gene            complement(1563..2885)
                     /locus_tag="PKL42_09345"
     CDS             complement(1563..2885)
                     /locus_tag="PKL42_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HNU67318.1"
                     /translation="MKLAALKGISFLLMSIAFAGQSHAANWVKLSENGTSKLLIDKQS
                     ILEKDQLTRAWVKVEYKKVQINSESADKTYNLSKLLWYFDCPAQKSATTQVLQYMNAE
                     TVFSAGIDVKAAEFIEPVPETDLDRAMRYVCTRAQPKKQQETKLASKPLESKPSETKA
                     QTAKTQENAPAEAEKKPEPAAAEAKPAESKQVEAKPEEVKAAPEKAAATKPTKGNTHA
                     AHWTYEGKEGPENWGKLQPEFAACETGQNQSPVDIDATIDAPLKTLKGIQKVPAKEIV
                     NNGHTIQANFKEGNMLLLDDTPFQMKQVHFHTPSENTIKGKPFPLEAHFVHADKKGNF
                     AVIAVMYKEGKANAGLEKLWSQMPKEKGAPVPLNTKVTAQELMPENRDYYRFSGSLTT
                     PPCTEGVRWLVLKTPMTVSKTQIAAFEEVLHHHNNRPVQPLNGRVVLE"
     gene            complement(3063..>3874)
                     /locus_tag="PKL42_09350"
     CDS             complement(3063..>3874)
                     /locus_tag="PKL42_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HNU67319.1"
                     /translation="LVGIANQKADDSIAPTKNTYLDLLWEPLAAHYEMPVLQPDAATN
                     SEDDIAFFMPRLVRLATPRVPSMLQNQPTLAQFLADTLANQPAEKMPDDAQRLQADIG
                     TLTHQYLELIAQHGLPAWPATRLASLKAAMQHWLQQRGHREIAAAGAADKIVTLLSTT
                     LNSQAGQWVLQANALGAQELALALVENNVVKNFVVDRTFVEKLADGTQVRWIVDYKTV
                     ELPMDITEAALKTIALQYAEQLEHYQQLFRHETLPIQKAIFFVSIGRLIQL"
BASE COUNT         1002 a          883 c          888 g         1101 t
ORIGIN      
        1 atgcgtattt gctgactgac cctgaccgaa aaattaccgt gcaagcgcaa aaacgcttgg
       61 atgcaattca actcatggaa gacttgggcg caggctttca ccttgccatg cacgatttag
      121 aaattcgcgg tgctggcgag ctattgggcg actcacaaag cggtgaaatg caagaaattg
      181 gctttcattt gtattcagat atgctcaacc acgccgtgaa gcaacttaaa gcaggtaaag
      241 agccagattt aaacgcaccc cttggcgtga ccactgaaat caacctgcac acgccagcgc
      301 tcttgcccac caaatattgc cccgatgtgc atgaacgctt agtagtgtat aaacgcttgg
      361 ccaattgcgt ggacgatgac gaactggata acctgcaaga agaactcatt gaccgctttg
      421 gcttgctgcc agagcaaggt gaggcgctca tggcgtgcca ccgattgcgt atcgcgtcaa
      481 aagcgattgg cgtgattaaa attgatgcga gtgatgcggc cattcagctg caatttatcc
      541 caaaagcgga tatcgaccca atgaaactca tcaacttatt gcaacgcgat aaacgttgcc
      601 gcatgaatgg gcctgataaa ttaagaatca ccgtgagctt tccgaccatt cacctacggg
      661 cagattttgt aaaaagtatt ttaagagaat ttatttagcc aaaaaaccaa cgattactag
      721 cggtaaatca aaaataaagc cgccacaatc gccacagtcg cccagccaat tctatccaca
      781 tattggtgta acttggcttc catacttggc ccgccccatt tcattaaccc tgccaccata
      841 aaaaagcgca tgccgcggcc aatgagggaa gccaaggtga aaggtatcaa ggccattgaa
      901 agtgtgccgg ctgcaatagt gaacactttg tagggaatgg gtgaaaagcc agcgataaaa
      961 atcgcccaaa aaccatagtc tttaaaccat gtgacggcgg taaggtacgc ttcccaataa
     1021 tggcttgctt tcaagtgcgg ctcaatcaca tcaaacatgg tgatgccaat aaaataacca
     1081 aatatgccgc ctaatactga agtcactgtg gtaatgagcg caaatcgcca ggctttattt
     1141 gggttggcca aactcatggg cgcgagcatt acatcaggcg gaatgggaaa aaatgaagat
     1201 tctgcaaaac tgagcgcgcc taaataccaa ggcgcattgg gtgatcttgc ccaaatcatt
     1261 gcgcgctgaa acaggccaga aaaaattttc attgggctat gtctttaaaa aatgagatgc
     1321 gatattctat cagtaaggtt gcccgatgcg tatgatttca ctgactagaa agttgtgttg
     1381 ttttagcggt tgatatgcat aaaataaaag ccaagaaaat atgcttttaa acctagcatt
     1441 ttgctacgag aaaaattttc tcctctgcga gcctttattt ataaggctta cagagcatcg
     1501 aaaaaggttg gtgttaacat gggtgtaaaa agcagtgttt taaaggctta aatttggtgc
     1561 cattactcca gcacaacacg gccattgagt ggctgtactg gccgattgtt atgatggtgt
     1621 aatacttctt caaaagctgc gatttgcgtt tttgaaaccg tcatgggtgt ttttaatacc
     1681 aaccaacgca cgccttccgt gcaaggcggt gttgtgagtg agccgctaaa tcggtagtaa
     1741 tcacggtttt ctggcattaa ttcttgtgca gtcactttgg tgttaagcgg tacaggcgcg
     1801 cccttttctt taggcatttg cgaccacagt ttttcaaggc ctgcgttggc tttgccttct
     1861 ttatacatca ccgcaatcac cgcgaagttg ccctttttat ccgcgtgcac aaaatgcgcc
     1921 tctagcggaa aaggtttgcc cttgatggtg ttttcacttg gagtatgaaa atgcacttgc
     1981 ttcatttgaa aaggcgtgtc atctagcagc agcatattac cttctttaaa attagcttgt
     2041 atggtgtgac cattattgac aatttctttt gccggcactt tttgtatgcc cttgagcgtt
     2101 tttagcggtg catcaatggt ggcatcaata tccacgggcg attgattttg cccagtttca
     2161 caagcagcaa attctggctg cagtttaccc cagttttccg ggccttcttt gccttcgtag
     2221 gtccaatgtg cggcatgtgt attacctttg gtaggtttag tcgctgccgc cttttctggg
     2281 gcagccttca cttcttcagg ttttgcctca acttgcttac tttctgctgg tttagcttcc
     2341 gccgctgcgg gttctggttt tttctcagct tctgcagggg cattttcttg tgttttagcc
     2401 gtttgcgctt tagtttctga gggcttgctt tctaacggct tgcttgccaa cttggtttct
     2461 tgttgctttt ttggctgcgc tctggtgcaa acatagcgca tcgcacggtc taaatctgtt
     2521 tctggtacgg gttcaataaa ctcggccgct ttaacatcaa tgcccgcaga aaacactgtt
     2581 tcagcattca tatattgaag cacttgcgtg gtggcggact tttgcgcggg gcaatcaaaa
     2641 taccagagca atttagacaa gttataagtt ttgtcagccg attcactatt gatttgcact
     2701 tttttgtatt caaccttcac ccaggcgcgc gtgagttggt ctttttctaa aatagactgt
     2761 ttatcaatca gtagctttga ggtgccgttt tcactgagct tcacccagtt tgcggcatgg
     2821 ctttggcctg caaaggcaat gctcatcaat aaaaaactaa tcccttttaa tgcagctaac
     2881 ttcatcacat tttggcccta acttaggtat ttatttaatt tcaatggcgt aaaaaaataa
     2941 gcgtttgaaa tacacaacac tgggcaacat cgcactttgc ccatgccaat tatggcaaat
     3001 ttgcgtgcct gatgatttga agagcaatgc gagatttagc cctcaattgt tgatttaaaa
     3061 cattaaagct gaattaaacg gccgatactg acaaagaaaa tagctttttg gattggcaat
     3121 gtttcatgcc tgaataactg ttggtaatgt tctaattgct cggcatattg caaggcaatg
     3181 gtcttgaggg ctgcctcagt aatgtccatc ggcaactcaa ccgttttata atcaacaatc
     3241 caacgcactt gcgtgccatc agcgagcttt tccacaaacg tgcggtccac cacaaagttt
     3301 ttcaccacgt tattttcaac cagcgcgagg gctaattcct gcgcgccaag cgcattggct
     3361 tgcaataccc actgccctgc ttggctgttg agtgtggtgc tcaacagggt aacaatctta
     3421 tccgcggcgc ctgcggcagc gatttcacga tgtccacgct gctgtaacca atgctgcatg
     3481 gcggctttta ggctagccaa gcgcgtggct ggccatgcag gcaagccatg ctgtgcgatg
     3541 agctctaaat attgatgcgt aagcgtgcca atatcggcct gtaaacgctg cgcgtcatcc
     3601 ggcatttttt cagcgggttg gttagctagc gtatctgcca aaaattgcgc aagtgtaggc
     3661 tgattttgca gcatggacgg cacacgaggc gtagcaagcc gcactaaacg tggcataaaa
     3721 aatgcaatat catcttctga gtttgtagca gcatcaggct gcaatactgg catttcataa
     3781 tgcgctgcca gtggttccca aagtaaatct aagtaggtgt ttttggttgg tgcaatgctg
     3841 tcatccgctt tttgattagc aatgccgacc aaat
//
LOCUS       DAOQSI010000176         3832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_26095_length_3832_cov_5.446651, whole genome shotgun sequence.
ACCESSION   DAOQSI010000176 DAOQSI010000000
VERSION     DAOQSI010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26095_length_3832_cov_5.446651"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..391
                     /gene="arfB"
                     /locus_tag="PKL42_09355"
     CDS             2..391
                     /gene="arfB"
                     /locus_tag="PKL42_09355"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HNU67320.1"
                     /translation="MPENEFDLTAIRAQGAGGQNVNKVSSAIHLRFDIAASSLSDWLK
                     ERLLHSKDNRITAEGMLVLKAQQYRTQEANKRDAIQRLHEIVNAANHVKPMRYATRPS
                     FGAKQRRLESKTQRGQIKQLRGKIAAE"
     gene            500..1189
                     /gene="tsaB"
                     /locus_tag="PKL42_09360"
     CDS             500..1189
                     /gene="tsaB"
                     /locus_tag="PKL42_09360"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNU67321.1"
                     /translation="MNLLAFDTSTEYLSLAVQKGSEVFTFDINAGQTHSQIILPQIQA
                     LLTEAKLALSDLQGIAFGAGPGSFTGVRIAAGVAQGLGFGANLPVVDICTLLALAEAS
                     GAGKVIACLDARMGEVYHAVYEKTHDTWQAVLSPGLYKPAEVPPVLGDGWVGAGSGWQ
                     AYSEQLSLVYGKQLHAVQPLALPTAAAILALAKPIVVSGNAKSAGEAMPIYIRNRVAL
                     KTAEREQGLKR"
     gene            1186..1674
                     /gene="rimI"
                     /locus_tag="PKL42_09365"
     CDS             1186..1674
                     /gene="rimI"
                     /locus_tag="PKL42_09365"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNU67322.1"
                     /translation="MSAMLQGKPQPSVTLRPMQQGDLDAIMQIEPQIYPYPWSRGNFS
                     DSLNSGYSAWVMQAQEETIGYALLMMVLDEAHLLNLSVAKSHQKQGLGRYLLAHMISV
                     AKKYKAANMFLEVRPSNVSAIALYESVGFNEMAIRRGYYPADPKQFKSGREDAVLMGL
                     AI"
     assembly_gap    1813..1912
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1913..2424
                     /locus_tag="PKL42_09370"
     CDS             <1913..2424
                     /locus_tag="PKL42_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67323.1"
                     /translation="PEVVQDIAAITIVEVATATPEAIHIEKIAAENNPAEIATQMLSH
                     IASDDGQWLFVLENHALSADEAKLLQNMQRAMRIQCQPSVQADHTLDLVNANNTQLIV
                     ALGEAAAQTLLQSTEKLTHLRGQLHPFQTAQLVVTFGLQHLLQHPQDKARTWEDICLA
                     MQALQINKI"
     gene            2747..3346
                     /locus_tag="PKL42_09375"
     CDS             2747..3346
                     /locus_tag="PKL42_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNU67324.1"
                     /translation="MQKIFLKLFAVLLVLNLTGCGYNAFQSLDETSKAAWSEVLNQYQ
                     RRADLVPNLVNVVKGYAEHEKDVLTQVTEARAKVGSMQITPEVLNNPDAFAKFQAAQG
                     ELSSALSRLMVVAENYPNLKADQSFRDLQAQLEGTENRITVARNRYIETIKEYNVAVR
                     SFPQNLTAMMFGYQTKPSFTVENEKAISTAPTVDFSDKK"
     gene            3369..>3832
                     /locus_tag="PKL42_09380"
     CDS             3369..>3832
                     /locus_tag="PKL42_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNU67325.1"
                     /translation="MLIHFKSRVSLGLCLLCLMLLLASSMVRAGPEISTESKAAEIAA
                     LVNNGIPALKAHVTDLTQTLNPAEQSALEAKLTALETEKGSQIGVLIVPTTQPETIEQ
                     YAIRVASQWKLGRAQQDDGVLLLVAKNDRKLRIEVGYGLEGAIPDVYAKRIVS"
BASE COUNT         1051 a          804 c          942 g          935 t
ORIGIN      
        1 aattcccgaa aatgaatttg atctcaccgc cattcgcgcg cagggcgcgg gtgggcaaaa
       61 tgtgaataag gtttcatcgg ccattcattt gcgctttgat attgccgctt caagtttgag
      121 cgattggttg aaagaacgtt tattgcacag taaagataac cgcattacgg cagaaggcat
      181 gttggtgctt aaggcgcagc aatatcgtac gcaagaggcg aataaaagag atgcgattca
      241 aagactgcac gaaattgtga atgcggctaa ccatgtgaaa ccgatgcgtt atgctacgcg
      301 cccgagtttt ggggcaaagc agcgcaggtt agaaagtaaa acacagcgcg ggcaaattaa
      361 acaattacgt ggcaaaatag cggctgaata acacttatga ctaaatatta ccgctcgtgg
      421 tgagcttgtc gaaccacaag taaacgtaaa acccttcggc aggctcaggg tgcacgggtt
      481 tggataattt ttgagaacaa tgaatttact cgcttttgac acctccaccg aatatttgtc
      541 gcttgccgta caaaaaggca gtgaggtgtt tacgtttgat atcaacgctg gccaaacgca
      601 ttcacaaatt attttgccgc aaattcaggc cttactcact gaagccaaac tcgcactgag
      661 tgatttgcaa ggcatcgctt ttggtgccgg gccaggttca tttaccggtg tgcgcattgc
      721 agccggtgtg gcgcagggtt taggttttgg tgcgaacttg cccgtggtag atatttgtac
      781 tttactggcc ttggccgagg ccagcggggc tggcaaagtg attgcctgct tagatgcacg
      841 gatgggtgag gtgtatcatg cggtgtatga aaaaacacat gacacttggc aagcggtttt
      901 gtcccctggg ctttataaac ctgccgaggt gccgcctgtt ctaggggatg gttgggtggg
      961 cgcgggcagt ggttggcaag cgtattcaga acaattaagt ttggtatatg gcaaacaatt
     1021 gcacgcggtg cagccactag ctttaccgac tgccgccgca attttggcgt tggcaaaacc
     1081 gattgttgtc agtggaaatg ctaaatctgc gggtgaggcc atgccgattt atattagaaa
     1141 ccgcgtggcc ttaaaaacgg ctgagcgcga acaaggctta aaacgatgag tgccatgctg
     1201 caaggcaagc cgcagccgag cgtgacatta cgccccatgc agcagggcga cttggatgcg
     1261 attatgcaaa tagagccgca aatttatccc tacccgtggt cgcgcggaaa ttttagcgat
     1321 tcgctgaatt caggctacag cgcttgggta atgcaagcgc aagaagaaac tatcggctat
     1381 gctttgctga tgatggtgct ggatgaagcg catttgctga atttgagcgt tgcaaaaagt
     1441 caccaaaaac aagggttagg ccgttatctg ctggcgcaca tgatttctgt tgcaaaaaaa
     1501 tataaggcag cgaatatgtt tttagaagtg cgaccaagca acgtttctgc tatcgctttg
     1561 tatgaaagtg ttggatttaa tgaaatggct atccgccgtg gctattatcc ggctgacccc
     1621 aagcaattta aatcgggccg cgaagatgca gttttgatgg gcttggcaat atgaggttta
     1681 gatgatgagc gcgcttaccc gtgaagatgt cctgcgtgag ctagaattgc tgcctgtgtg
     1741 gcagttgcgc cagcctctgc ctgaagtggt gcaagatatt gcagcaataa cgattgttga
     1801 ggtggcaaca gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntgcctgaa
     1921 gtggtgcaag atattgcagc aataacgatt gttgaggtgg caacagctac gccagaagca
     1981 atccacatag aaaaaatagc cgcagaaaat aatccagccg aaatcgctac gcaaatgtta
     2041 agtcatatcg ccagtgacga tggacaatgg ttgtttgtgc ttgagaatca tgcgctgagt
     2101 gcagatgaag ccaagctact gcaaaacatg cagcgcgcta tgcgcattca atgccagcct
     2161 tccgtgcagg cagatcacac actggatttg gtgaatgcca acaacacgca gctgattgtt
     2221 gcgctgggcg aggcggcggc gcaaacgctt ttgcaatcca ctgaaaaact cacccatttg
     2281 cgcggtcagt tgcatccatt tcaaaccgct caactggtcg tcacttttgg cttgcaacat
     2341 ttgttacagc acccacaaga caaagcgcgt acttgggaag atatttgtct agccatgcag
     2401 gcgctgcaaa tcaataaaat ctgatggctg aaagcaattg taaacaattg aaatttgcaa
     2461 attcaacccc aagtaatcaa ccgtataatg cgtttaaaac aagtggtttt cacatcaacc
     2521 cttttgagca ggcctgtagg cctttattta taaggctcgc agggcagtgt tttttgctcg
     2581 gtagatattg tgaatgaaaa gcgcgatttt tcgccaacaa aaatcatcca atttatcgtg
     2641 ctaaattgcg cctcatgaat cacgaatata gaactggctt tcagtgttgt cactaattta
     2701 gtatcatcaa tattggcttt gtcttttgac ttataaagga atgaacatgc aaaaaatatt
     2761 tttaaaactt ttcgcagtgc tgttggtatt gaacttaacg ggttgcggtt ataacgcgtt
     2821 ccaaagttta gatgaaactt caaaagcggc atggtctgaa gtgttaaacc aatatcagcg
     2881 tcgtgctgat ttagtgccaa atttggtgaa tgtcgtgaag ggctatgcgg agcacgaaaa
     2941 agacgtactc acacaagtga cagaagcgcg cgccaaagtg ggcagcatgc aaattacccc
     3001 cgaagtactt aataaccccg atgcgtttgc caagtttcaa gcggcacaag gtgaactcag
     3061 cagtgcgcta tcgcgcttaa tggtggtggc tgaaaattac ccaaacttaa aagccgacca
     3121 aagctttaga gacttacaag cacagcttga aggcactgaa aaccgcatca ccgttgcgcg
     3181 taatcgttat attgaaacca ttaaagaata caacgttgcg gtgcgttctt tcccacaaaa
     3241 cttaacggcg atgatgtttg gttatcaaac caaaccgagc tttacagttg aaaacgaaaa
     3301 agccatttca acagcgccaa cggtagattt tagcgataaa aaataagcta aaatgatttc
     3361 aacccctcgt gttgattcac tttaaatcgc gtgtcagttt aggcctgtgc ctactttgct
     3421 tgatgctgtt attggcatca agcatggtgc gcgctgggcc agaaatttcg acagaatcaa
     3481 aagcggcaga gattgccgct ttggtgaata acggaattcc agcactaaag gcgcatgtca
     3541 cggatttaac gcaaacgtta aaccctgctg aacaaagtgc gttagaagcc aaactaaccg
     3601 ctttggaaac tgaaaaaggc agtcaaattg gggtgttgat tgtccccacc acgcagcccg
     3661 aaaccatcga acaatatgcc atccgcgttg caagccaatg gaaacttggc cgagcgcaac
     3721 aagatgatgg cgttttgttg ctggtggcca aaaatgaccg aaaactgcgt atcgaagtcg
     3781 gttatgggct agaaggcgca attcctgatg tgtacgccaa gcgcattgtt tc
//
LOCUS       DAOQSI010000177         3816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_26255_length_3816_cov_3.927147, whole genome shotgun sequence.
ACCESSION   DAOQSI010000177 DAOQSI010000000
VERSION     DAOQSI010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26255_length_3816_cov_3.927147"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            402..2363
                     /gene="acs"
                     /locus_tag="PKL42_09385"
     CDS             402..2363
                     /gene="acs"
                     /locus_tag="PKL42_09385"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009852215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNU67326.1"
                     /translation="MSNESKEIRVFHPSENTVKQATVSGMAAYDALYAEAEKDYEGFW
                     AKLARELLVWHKPFTQTLDESKAPFYRWFADGELNVSYNCLDRHLSKNGDKTAIIFEA
                     DDGSSTAVSYRSLYHQVCKFANGLKSLGLNNGDRVVIYMPMGVEAVVAMQACARLGLI
                     HSVVFGGFSAKSLVERVHDAAARVIVTCDGQFRGGKQMPLKTAVDEALQDPLCNSVEK
                     IVVFKRTGGEVTWDAKREIWWDELIANQADTCDPVWVSAEHPLFLLYTSGSTGKPKGV
                     QHSSGGYLLHAMNTMRWTFDIKDNDVFWCTADVGWITGHSYVAYGPLAMGATQVVFEG
                     IPTYPDASRFWKVIEKHKVTIFYTAPTAIRSLIKLGGDMPRQHDLSSLRILGSVGEPI
                     NPDAWMWYYNEVGGGRCPIVDTFWQTETGGHAITPLPGVTPLVPGSCTLPFPGMKITV
                     VDESGVEVEWGTGGFLVIKKPWPSMIRNIWNDPERYKQSYFPEDLGGKLYLAGDGAIR
                     DKETGYFTIMGRIDDVLNVSGHRMGTMEIESALVANSLVAEAAVVGKPHDIKGEAIVA
                     YVVLKGERPEGDEAKKIVAQLRDWVGKEIGPIAKPDDIRFGDNLPKTRSGKIMRRLLR
                     SLAKGEEITADVSTLENPGILDQLKQTVA"
     gene            2478..2804
                     /locus_tag="PKL42_09390"
     CDS             2478..2804
                     /locus_tag="PKL42_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67327.1"
                     /translation="MALKPITLRLDEEEYDKLKAHLEAFGDPDINVAYVVRSYIRDLN
                     RSLPFMLNSGWDLKNYFGMLGSWLKQFGAMKDADMFKMMNNPWQMWTSGTSDKSADDS
                     SNTDKQ"
     gene            2844..3287
                     /locus_tag="PKL42_09395"
     CDS             2844..3287
                     /locus_tag="PKL42_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67328.1"
                     /translation="MMTEQQMRMMIEMFSNPMFKNGANEFLKIAQQQGMEAANKFWGN
                     SSESQDFPGVDKMMERMAEFYQTLGFVTQAKYDELMKQFASLKAENQMLSDTIRELQQ
                     RFIAENGAKAQQAWQEVVDKQLEMSREVTKSFFDVLKQSSPEKSK"
     gene            3339..3695
                     /locus_tag="PKL42_09400"
     CDS             3339..3695
                     /locus_tag="PKL42_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67329.1"
                     /translation="MDKQLEYFDVLTDTQKQVFNNLLNAQKDLRVQWIEAIGKTHAAF
                     TTIPGLPETPQTKEALNQFNTWFSTVASNSQSATEEALKVQENCISAYEKQLAISREV
                     LKSFIDIANPAKAKTA"
BASE COUNT         1163 a          751 c          853 g         1049 t
ORIGIN      
        1 caactgattt tggtgaaaac aatggcttaa gatggtgttg actcatgatt tctctaattc
       61 tctttaagcg tcagtttgaa tattagcgta atgcaagcaa tgggtagatg agtttcataa
      121 aataaaatcc atctagcctg cttattaact tgcgattatt gtacctttag cagtcatagt
      181 ttacacctaa atttaataat ttatcatata tcttatgtct tatataagat atatgttgac
      241 agacttgttg gtagcgtatt attattactt ttcggctgat aagttatgct cttcatatac
      301 caatcatcca atggcaaagc agctaacaag ttgatgtatt tatttgaata aaagctgatg
      361 tcgcactttt aatcgtatca agtacttttg gaggagacag catgagtaac gaatcaaaag
      421 aaattcgcgt ttttcaccct agtgagaaca cagttaaaca agcaactgtt tcaggcatgg
      481 cagcttatga tgcgctttat gctgaagcag aaaaggacta tgagggtttt tgggcaaagc
      541 tagcacgcga attattggta tggcacaagc cttttactca gactcttgat gaaagcaaag
      601 ccccattcta tcgttggttt gcagatggcg aactcaatgt ttcctataac tgcctagatc
      661 gacacttgtc aaaaaatggc gataaaactg ccattatatt tgaagctgac gatggcagtt
      721 caaccgcagt cagctatcgt tcgctatatc atcaagtgtg taaatttgct aacgggctta
      781 aatcattagg tctaaataat ggcgaccgtg tagtcatcta catgccaatg ggagttgagg
      841 cagtggttgc tatgcaagca tgcgcccgcc tcggattaat acattcagta gtatttggcg
      901 gcttctctgc caagagtcta gtcgagcgtg ttcatgatgc tgccgcgcgc gttatcgtaa
      961 catgtgatgg acaatttcga ggtggcaaac aaatgcccct caaaacagcg gtggatgaag
     1021 ccctgcaaga cccgttatgc aattctgtag aaaaaatcgt agtgttcaag cgtacaggtg
     1081 gcgaagtcac ttgggatgct aagcgtgaaa tatggtggga tgagcttatc gcaaatcaag
     1141 cagatacctg cgacccagtc tgggtcagcg cagagcatcc gctattctta ctttatacat
     1201 ctggttctac aggcaaacct aaaggtgtac agcactcttc aggtggttat ctattacatg
     1261 cgatgaacac catgcgctgg acttttgata ttaaagacaa tgatgttttc tggtgtaccg
     1321 ctgatgtagg ctggattact ggtcatagtt atgtggccta tggcccgttg gctatgggcg
     1381 ccactcaagt cgtatttgaa ggcattccga cttatcctga tgcctcgcgc ttctggaaag
     1441 tcatagaaaa acataaagtt actatttttt atactgcgcc tacagcaatt agatcgctta
     1501 ttaagttggg tggcgacatg ccacggcagc atgatttatc gagcttgcgc attctcggtt
     1561 cggtaggcga gcctatcaac ccagatgctt ggatgtggta ttacaatgaa gtgggtggag
     1621 gccgttgccc tattgttgac actttctggc agacagagac tggcggccat gccattacgc
     1681 ctttgcctgg cgttactccg cttgtacctg gctcttgtac actgcccttc cctggcatga
     1741 aaatcactgt ggtggatgaa agcggcgtag aagtggaatg gggtactggc ggcttcttag
     1801 tcatcaaaaa accttggcca tccatgatcc gtaatatttg gaacgaccct gaacgttaca
     1861 agcaatctta ctttcctgaa gatttgggcg gcaagctata tctcgctggc gatggcgcca
     1921 tccgcgataa agaaacaggt tacttcacta ttatgggccg cattgatgat gtattaaatg
     1981 tttctggcca tcgtatgggg accatggaaa ttgaatctgc actggtagct aactcactag
     2041 tggcagaagc ggctgtagta ggtaaaccgc atgacatcaa gggtgaggca attgtggcct
     2101 acgtggtatt aaaaggtgaa cgtccagaag gcgatgaggc caaaaagatt gtggcacagc
     2161 ttcgtgattg ggtgggcaaa gaaattggcc ctatcgccaa gccagacgat atccgctttg
     2221 gtgacaactt acctaaaacg cgctctggta aaatcatgcg ccgcttattg cgctcattag
     2281 caaaaggtga agaaattacg gccgacgttt ctacgttgga aaatccagga atattggacc
     2341 agctcaaaca aactgtagct taagcacctc aacccaaagg tttaagacta tttttataaa
     2401 aataatcatt ttatagttga ctagtattac ttttgtagta ctataagcat actttaaatg
     2461 tatttattgg aataaaaatg gctctcaaac ctatcacctt aagattagac gaagaagaat
     2521 acgataagct caaagcgcat ttagaagcct ttggcgatcc agatattaac gttgcttacg
     2581 tggttcgttc atatattcgt gacctaaata gatcattgcc attcatgctt aactcaggct
     2641 gggatcttaa aaattacttt ggcatgttgg gctcttggtt aaaacaattt ggcgcgatga
     2701 aagatgccga tatgttcaag atgatgaaca acccatggca aatgtggaca tcaggcacta
     2761 gcgacaaatc tgcagatgat agtagcaaca ctgataaaca gtaatgcttt aactaattaa
     2821 gcaagttaaa aggaaaccta gccatgatga cagaacagca aatgcgcatg atgattgaga
     2881 tgttctctaa ccctatgttt aaaaacgggg caaatgagtt tttaaagatt gcacagcaac
     2941 aaggcatgga agctgctaat aaattctggg gcaattcatc agaaagccaa gattttccgg
     3001 gggtagacaa aatgatggag cgcatggcgg agttttacca aacgctaggt tttgtgacgc
     3061 aagctaagta tgacgagcta atgaagcaat tcgctagctt aaaggccgaa aaccaaatgc
     3121 taagcgacac aatacgagaa ttacaacagc gttttatcgc tgaaaatggc gcaaaagcgc
     3181 aacaagcatg gcaagaagtc gtcgacaaac agcttgagat gagtcgcgaa gtcactaaga
     3241 gtttttttga tgtacttaag cagtcctcgc ccgaaaagag caagtaactt aaacaagtca
     3301 tttaaggcga attattttta actttgaaag gaactaaaat ggataaacaa cttgaatatt
     3361 ttgacgtttt aactgataca cagaaacaag ttttcaataa tttattgaat gctcaaaaag
     3421 atttacgcgt tcaatggata gaagcgattg gtaaaacaca tgcagctttt actactattc
     3481 ctggcttgcc agaaacaccg caaactaaag aagcgttgaa tcaattcaac acttggttta
     3541 gcacagtagc tagcaattca caaagcgcaa ctgaagaagc acttaaagtg caagaaaact
     3601 gcatcagtgc ttatgaaaaa caattggcaa tcagccgtga agtattaaaa agcttcattg
     3661 acattgctaa cccagcaaaa gctaaaacag cttaagttga gccatgcaag catgcattgg
     3721 ttgcctgaag gcgactgatg caagctctgc ccctattact aattattttg catctagcca
     3781 actcattcaa tgagccatag ggcaaaactt aagagg
//
LOCUS       DAOQSI010000178         3799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_26441_length_3799_cov_4.122062, whole genome shotgun sequence.
ACCESSION   DAOQSI010000178 DAOQSI010000000
VERSION     DAOQSI010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26441_length_3799_cov_4.122062"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..518)
                     /locus_tag="PKL42_09405"
     CDS             complement(147..518)
                     /locus_tag="PKL42_09405"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HNU67330.1"
                     /translation="MTAINKQHIATLKLAMESELARLLHEVKDEMDPALRPSFADVSG
                     EVTDSGDESIAESVVDLDNAMIGLHLQKVNDLYAALDRIQAGVYGICGDCGESVSVER
                     LTAYPTAKRCINCQTVREKRA"
     gene            658..1587
                     /locus_tag="PKL42_09410"
     CDS             658..1587
                     /locus_tag="PKL42_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNU67331.1"
                     /translation="MGFLSDKQHVNWLQWARWLIFASCLTSTTLAFAQSASWQLSGNV
                     FEYTVQAGDTLTKISARFGISSAVLARENGLKPNAALTPEQTLQIDNRHIVPDAQGLD
                     LLINLPQRMLYFYQDGLLNLTYPVALGKPTWPTPTGEFTVKNKIANKTWIVPISIQEE
                     MRREGKAVKTHVPPGPNNPLGKYWLGLSLSGIGIHGTTAPASIYRFQSHGCIRLHPDD
                     IEVLFNQIEPEATGNIIYAPLLLAESEGRIFVEAHQNIYKNQRAYRKLDASLKALEQM
                     ANDAMLSERIDWERAKHVLEQRDGIARDVTREY"
     gene            1614..2180
                     /locus_tag="PKL42_09415"
     CDS             1614..2180
                     /locus_tag="PKL42_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HNU67332.1"
                     /translation="MKNEICKTRRNLLKSSMALALAGLSSRPALATFKPQDAKDTIVR
                     ELAFYNLHTGENLKAEYWSEGGYIPDALAGIDHILRDFRTDEVLPMDPQLLDLLHSLR
                     STLGTNQPFQIISGYRSPKTNANLAANSGGVAKGSLHMQGKAIDIRIEGTPLKHVREA
                     AIALQGGGVGYYPDSNFVHMDVGRIRTW"
     gene            2273..2893
                     /locus_tag="PKL42_09420"
     CDS             2273..2893
                     /locus_tag="PKL42_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67333.1"
                     /translation="MNDRIQQLLAQITTLEDELRTALNEQQSSIFFQIKGKRVEFEQS
                     IKETHRRLKRNFFRWLITDRPQNLITGPIIYSMIVPLLITDLFITFYQLTCFPIYGIK
                     KVKRSDYIIFDRQQLNYLNFIEKFHCTYCAYGTGMIAYISEIVARTEQYFCPIKHARK
                     ILGTHSRYAHFLEYGEADNYEAKLEAIRVALNKEYQKDQSSSGDCK"
     gene            2890..>3799
                     /locus_tag="PKL42_09425"
     CDS             2890..>3799
                     /locus_tag="PKL42_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNU67334.1"
                     /translation="MKITFLGATGTVTGSKYLLTSESSQGDEKRVLIDCGLFQGLKQL
                     RLRNWAALPINTQEIQTVILTHAHIDHSGYLPLLVKNGFSGKVYCTEATRDLCEVLLL
                     DAAYLQEEEARYANKGGFSKHTPALPLYTQEDAQKAIALLTPVPYEQDIDLGDGLFAK
                     LYPNGHILGSAFVRIHNQKTSIVFSGDIGRPHDILMKAPAIIQQADYLVLESTYGNRL
                     HDKSDPKIKLAEIINQTVKRKGVVIIPVFAVGRAQELLYYIHLLKASNAIAKDTAVYL
                     NSPMAVDATAIFNHFKDEHRLTHEQSQ"
BASE COUNT         1119 a          893 c          777 g         1010 t
ORIGIN      
        1 ttcattgcca acctttttcg ctgccctgcg agccttataa ataaaggctc gcagggcatt
       61 aaatttttct catgtgaaag tggcaatata aattacatat ttcagcccga ttttttcgtg
      121 tttttttgat ttgatataaa tagcatttat gctctttttt cacgcactgt ttgacagtta
      181 atacagcgtt tagctgtcgg gtaggccgtt aatcgctcta cactgaccga ttcaccacag
      241 tcaccacaaa tgccataaac accggcctgt attctatcga gtgcagcata taaatcattc
      301 actttttgca gatgcaaacc aatcatggcg ttgtccaaat ccaccacaga ttccgcaata
      361 gattcatctc cagaatcagt cacttcgccg ctcacatcgg caaaactcgg cctgagtgct
      421 gggtccatct catcttttac ttcatgcaac aatcgcgcta actcagattc catcgcaagt
      481 ttaagtgtgg caatgtgttg cttgttgatt gcggtcatag tatctcctat actgtttttt
      541 aatttgaacc aataaaagct tattgttcca atataaacat tcattccaca tttaagaatg
      601 cgtgctagca cgtagaggta tactgtcgat taactgttaa actggtgata ttaatcaatg
      661 ggttttttaa gcgataaaca acatgtaaac tggttgcaat gggcacgctg gttaatattt
      721 gccagttgcc tgacaagtac cacacttgcg ttcgcccagt ctgcatcttg gcagttaagt
      781 ggcaacgtat ttgagtatac agtgcaggct ggtgatacac tcactaaaat tagcgcacgt
      841 tttggtatat caagcgcggt gttagcgcgg gaaaatggct taaagccaaa tgcagcacta
      901 acacccgagc aaaccttgca aatcgataat cggcatatcg tgcccgatgc tcaggggctg
      961 gatttactta tcaacctgcc gcagcgcatg ctctattttt atcaagatgg gctattgaat
     1021 ctgacctatc ctgtggcgtt gggtaagccc acttggccaa cacctacagg ggagtttaca
     1081 gttaagaaca aaatagccaa taaaacttgg attgtgccga tatcgattca agaagaaatg
     1141 cgacgcgaag gtaaagccgt taaaacgcat gtaccgcccg gcccaaataa tccgctgggt
     1201 aaatattggc tgggtttaag cctgtctggt atcggcattc atggcaccac ggcacctgcc
     1261 agtatttatc gctttcaaag tcacggctgc atccgtttgc atccagatga tattgaagtt
     1321 ttgtttaacc agattgaacc agaagccaca ggcaatatta tttatgcccc tttgctttta
     1381 gcagaatctg agggtagaat ttttgtcgag gctcatcaaa acatttataa aaaccagcgt
     1441 gcgtatcgca agctggatgc ttcactaaag gcacttgaac aaatggcaaa tgacgccatg
     1501 ttaagtgagc gcatcgattg ggagcgcgca aagcatgtgc tagaacagcg cgacggcatt
     1561 gcaagagatg tcacgcgcga atattagctt aatcaataaa gaggtaccaa accatgaaaa
     1621 atgaaatttg caaaacacgc cgcaatctac tgaaaagtag catggcgctc gctttggcag
     1681 gccttagcag tcgccctgcg cttgctactt tcaagccaca agatgccaaa gatactatcg
     1741 ttcgcgagtt ggctttttat aatctacaca ctggcgaaaa tctcaaagcc gaatattggt
     1801 ctgaaggtgg ctacattcca gatgcactgg ctggcattga ccacattttg cgcgattttc
     1861 gtacagatga agtgttgcca atggacccgc aactactaga tttgcttcat agcttacgta
     1921 gcacactcgg taccaatcaa ccatttcaaa ttatttcagg ctaccgctca cccaaaacca
     1981 atgcaaactt agccgctaat tctggcggcg tcgccaaagg cagtttgcac atgcaaggta
     2041 aagccattga tattcgcatt gaaggcacgc ctttaaaaca tgtgagagaa gctgcaattg
     2101 cgttgcaagg cggcggtgtc ggctattacc cggattcaaa ttttgtgcac atggacgttg
     2161 gtcgaattag aacctggtaa acttaccatg tgagtgctta ctctcacctc aaaagagtaa
     2221 gcatcgacat ctgtgcgtta aataataatg tgccataaaa actggagcac cgatgaacga
     2281 ccgtattcag caactgttag cccaaatcac cacgctcgaa gacgaacttc gcacagccct
     2341 taacgaacaa caatccagta tattttttca aattaaaggt aaacgcgttg agtttgaaca
     2401 atccatcaaa gaaacgcatc gtcggctaaa acgaaatttc tttcgctggc tcatcaccga
     2461 taggccacaa aacttaatta cggggcccat tatttattca atgattgtgc ctttactcat
     2521 cacggattta tttatcacct tttatcaact gacctgcttt ccaatctacg ggattaaaaa
     2581 agtaaaacgt tctgactaca ttatttttga ccgtcagcag ctcaattatc ttaattttat
     2641 tgaaaagttt cattgcactt actgcgccta cggcacaggc atgattgcct acattagtga
     2701 aattgtggcg cgaactgagc aatatttctg ccccattaaa cacgcacgca aaattttagg
     2761 cacacatagc cgttatgcgc actttctaga atacggcgaa gctgacaact atgaagcaaa
     2821 actggaagca atacgcgtag cgctcaataa agaataccaa aaagaccaat ccagctcggg
     2881 agattgcaaa tgaaaatcac gtttctaggc gctacaggca cggtaacagg ctctaaatat
     2941 ctcttaacaa gtgaatcatc ccaaggggac gaaaagcgcg ttttaataga ctgtggctta
     3001 tttcaagggc ttaaacaatt acggctgaga aactgggccg cattgccaat taacacgcaa
     3061 gaaatccaaa cagtcatctt gacccacgcg cacattgacc acagtggcta tttaccattg
     3121 ttagttaaaa atggcttttc tggcaaagtg tattgcaccg aggccacacg cgacctgtgc
     3181 gaagtcttac ttttagacgc agcttatttg caggaagagg aagcgagata cgccaataaa
     3241 ggtggatttt ctaaacatac ccccgcgctg ccgctttata cgcaagaaga cgctcaaaaa
     3301 gccatagcac tactaacacc tgtgccgtac gagcaagaca ttgatttagg cgatggctta
     3361 ttcgcaaagc tatatccaaa tgggcacata ttgggctcag cttttgtgcg cattcacaac
     3421 caaaaaacgt caattgtttt ttctggtgac attgggcgac cacacgacat tttgatgaaa
     3481 gcccctgcaa tcatccaaca agctgactat ttagtgctgg aatccactta cggtaatcgc
     3541 ctgcacgaca aatcagaccc caaaattaaa ctcgctgaaa ttatcaacca aaccgtcaaa
     3601 cgcaaaggcg ttgtcatcat tcccgttttt gccgtgggcc gcgcgcaaga gttgctgtat
     3661 tacattcacc tacttaaagc atctaatgcc atcgccaagg acacggctgt ttaccttaac
     3721 agccccatgg cagttgatgc taccgccata tttaaccact ttaaagatga acatcgctta
     3781 acgcatgagc aaagccagg
//
LOCUS       DAOQSI010000179         3779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_26631_length_3779_cov_4.881310, whole genome shotgun sequence.
ACCESSION   DAOQSI010000179 DAOQSI010000000
VERSION     DAOQSI010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26631_length_3779_cov_4.881310"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="PKL42_09430"
     CDS             complement(<1..895)
                     /locus_tag="PKL42_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNU67335.1"
                     /translation="MTGEPSGLIAMTAGESAASAIAGNIFNSLLKYDKNLDLAGELAE
                     YWDISKDKKTITFHLKPNLKWADNQPLTSEDVLFTWQKVTDDNTRTPYGADYKLVIKA
                     ETPDANTFKITYAAPYAPALETWASLHILPKHLLKDQDINTTAFARNPVGSHYYKLEK
                     WKNGQYLTLVRNPNSTQGQAYIEKLTSRIIPDKAAQFLELSADNIDIMGLDSIQYSRV
                     LPARPELNKNIALYKELGNSYVYLGFNLKRQPFNDVRVRQAINYAINKQEIIEGVLLG
                     LGEPVASPYKPGTRWSNPALSP"
     gene            complement(1075..2958)
                     /locus_tag="PKL42_09435"
     CDS             complement(1075..2958)
                     /locus_tag="PKL42_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNU67336.1"
                     /translation="MIKNTSFQHPITKSTSSINWLAVLLCMSLMCGLTQAETNVLGQV
                     ISPSNSPEKTQDNQSDTNVNQTPVNIEAHESPIFVTPENTSGELIFPEDADLTVNTET
                     TAEDADPYSNIITIESDDLWERIKAGYGMQDLSSSLVTQHEKQYSANPEYMRRMVERS
                     QRYLFHIVEEVEKRGMPTEIALLPMVESAFNPQAYSKSHASGIWQFIPSTGKVFGLKQ
                     NWWVDNRRNITVATDAALNYLQKLYGMFGSWDLALAAYNAGEGTVGRAIEQNRKQGLA
                     TNYESLNLPAETRNYVPKLQAIKNLMTNPGKYGLKIQTIANTAYFTKITAPPQIDARL
                     AAKLAEISDAEFLALNPSYNRPVIASANERHELLLPIASAQIFRENLAAYNKPLVTWK
                     TYQAKRGERMDKVASKFGIQVEQLRNANNLPKTEKFTTASTILVPKEKSADTSQSKTF
                     DASTDPIQKITNEDEVLKAQNAANIDLTHTEKLALSSTKDTQNAADSEARKNNTNKIE
                     PTIERDVTHKIKKGETLQSIARRYDVSVKQILAINTLKSTQVKVGQTLKIKTEVVETP
                     KQSSEGKVTHKKKAANKHHQVKSNAASNKNKVKQESSHKKSQTKAKKQQQTERTKKQP
                     KNK"
     gene            complement(3132..>3779)
                     /gene="gloB"
                     /locus_tag="PKL42_09440"
     CDS             complement(3132..>3779)
                     /gene="gloB"
                     /locus_tag="PKL42_09440"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HNU67337.1"
                     /translation="LGLTLNSILITHHHHDHIGGVTALLIQFPDCVIYAPALERYSFK
                     HIQVCQSEVVVIDALNLQFTVLDVPGHTIGHVAYVAEPANDSPILFCGDVLFGAGCGR
                     VFDNNYIKAYLSLQKLATLPPTTKIYCTHEYTLNNIQFALTLEPNNQALQTRHLATQQ
                     LRKDQVPTLPSNIALELATNPFLRCTSQEIIASIQALNETPERVFTLIRKLKNYY"
BASE COUNT          935 a          692 c          877 g         1275 t
ORIGIN      
        1 agggagagag cgctgggttg ctccagcgcg tgccaggttt gtagggggat gcaacaggtt
       61 cgcctagccc taacaaaacg ccctcgataa tttcttgctt attgatcgcg taattaatcg
      121 cttggcgaac gcgaacatca ttaaatggct gccgtttaag gttgaagcct aagtaaacgt
      181 aactattacc tagctcctta tacaaggcaa tatttttatt caactcaggt ctagctggca
      241 aaactcttga gtattgaatg gaatcaagcc ccatgatgtc gatattatca gcacttaact
      301 ctaaaaattg tgcagcttta tccggaatga tgcgcgaggt gagcttctct atataagctt
      361 gaccttgtgt tgaattggga ttgcgcacca gtgttaaata ttgcccattt ttccactttt
      421 cgagtttgta atagtggcta cctacagggt tacgtgcaaa cgcggtggtg ttgatatctt
      481 ggtctttaag taggtgttta ggcaaaatgt gcaagctggc ccatgtttct aaggccggtg
      541 cataaggtgc cgcataggtg attttaaagg tatttgcatc gggcgtttca gctttaatga
      601 caagtttgta atcagcacca tacggtgtac gcgtattgtc atcagttact ttttgccaag
      661 taaacagcac atcctcactg gtgagaggtt gattgtctgc ccattttaaa ttgggtttaa
      721 gatgaaaggt gatggttttt ttatctttag aaatatccca atattctgcc aactcacccg
      781 ctaaatcgag atttttatca tacttaagta agctattgaa aatatttccc gcaatggctg
      841 aagcagcaga ttcaccagct gtcatggcaa ttaacccgct aggttcaccc gtcatcgcgt
      901 tgatgagcgt gccgccttca gtagcaggat gctgcttgct gtaatctatt tgtgctcgtt
      961 gatgattggc attgtcacag ccattcaaca aaaataagct caacaacaaa agtaggataa
     1021 tttcgactgg acgtatgtca tattgcgttg ggctgagttt taaaaacata ttcatcattt
     1081 attttttggt tgttttttgg ttcgttcggt ctgttgctgt tttttagcct tcgtttgcga
     1141 tttcttgtgt gatgattctt gttttacttt gtttttgttt gaagctgcgt ttgatttcac
     1201 ttgatgatgt ttgttagcag cctttttttt gtgggttact ttgccttcgc tcgattgttt
     1261 tggcgtttca actacctcag ttttaatttt taaagtttgc cctactttaa cttgggtgct
     1321 tttaagggta ttgatggcta aaatttgttt tacgctgaca tcataacgtc tagcgataga
     1381 ctgtagggtt tcaccttttt taattttgtg tgtgacgtct ctttctatgg tgggttcaat
     1441 tttattggta ttattttttc gcgcttcact gtcggctgca ttttgtgtgt ctttagtgct
     1501 tgataaagcg agtttttcgg tatgcgtgag gtcaatattg gctgcgtttt gggctttcaa
     1561 tacttcatct tcatttgtaa tcttttgtat agggtctgtg gatgcatcga aagttttgct
     1621 ttggctagta tcggcagatt tttctttggg taccaaaatg gtagaagccg ttgtgaattt
     1681 ttcagttttc ggcagattgt tcgcattgcg caattgctcc acttgaatgc cgaactttga
     1741 ggcaacttta tccatgcgtt cgccgcgttt tgcttgatag gttttccaag tgacaagcgg
     1801 tttgttgtag gcggccaagt tttctctgaa gatttgggcg gaagcaattg gtagcaataa
     1861 ctcatgtctt tcgttagcgc ttgcaatgac agggcgatta taactggggt ttagggctaa
     1921 aaattcagcg tcactgattt cagctaactt tgcggctaaa cgtgcatcga tttgcggcgg
     1981 ggcagtgatt ttggtgaaat aagcagtgtt ggcaatggtt tgaattttaa ggccgtattt
     2041 gcctggattc gtcattaaat ttttaatggc ttgtagcttg ggaacataat ttctggtttc
     2101 tgccggtaag ttcaggcttt cataattggt ggcaagccct tgcttacgat tttgttcaat
     2161 cgcacggcct accgtgcctt ccccggcatt ataagcagca agcgctagat cccaactgcc
     2221 aaacatgcca tatagcttct gcagataatt tagggctgca tcggtggcaa cggtgatgtt
     2281 acggcggtta tcaacccacc agttttgttt taagccgaat accttgccgg tagaggggat
     2341 gaactgccag atacctgatg catgactttt tgagtaagct tgcgggttaa atgcgctttc
     2401 caccattggt aataaagcaa tttctgtagg cataccgcgc ttttccactt cttctacaat
     2461 gtgaaataaa tagcgctgac tgcgctcaac catacgcctc atgtactctg gatttgcgct
     2521 gtactgtttt tcatgttgcg taactaggct ggaagataaa tcttgcatgc catagccagc
     2581 tttgatgcgt tcccataagt catcgctttc gatggtgatg atattggaat atggatcagc
     2641 atcttcagct gtagtctctg tgttaacagt gaggtcggca tcttcaggaa aaattagctc
     2701 gccagaggta ttttctggcg ttacaaaaat tggggattca tgggcctcta tattaaccgg
     2761 cgtttgattg acgttggtat ctgattgatt atcttgggtt ttttcaggtg aattgctagg
     2821 actaattact tggccgagta cattcgtttc tgcttgcgta agcccacaca ttaacgacat
     2881 acacaacagc accgcaagcc aattgatgct tgatgtgctt ttggttatgg gatgctgaaa
     2941 agatgtgttt ttaatcaaga atatccttgc gaaaactaaa gcgatgaaaa tacggtgtgc
     3001 cgatgataaa agtggataga gtagtaatca ttacccactg ttaaacggaa cagcatggta
     3061 acgaaactta tacacctaag tcaagcgcgt tataatataa aatactttaa ttgtatattt
     3121 taaatatatg attaataata attttttaat tttcttatta atgtaaagac acgttcaggc
     3181 gtttcattta aagcttgtat acttgcaata atttcttgac tggtgcagcg taggaatggg
     3241 ttggttgcca gctctaatgc aatgttggag ggcaatgtcg gcacttgatc ttttcttaac
     3301 tgctgtgttg ctaggtgacg tgtttgtaat gcctgattat tgggttctaa agtgagcgca
     3361 aattgaatgt tattgagtgt atattcatgg gtgcagtaga ttttggtagt tggtggtagg
     3421 gtggcgagct tttgtagtga aagatacgct ttaatgtagt tattatcaaa aacacggccg
     3481 catccagcgc caaatagcac atcaccacaa aacaatattg gtgaatcgtt tgcaggttct
     3541 gcaacataag caacatggcc gatggtatgc ccgggtacat ctaagacagt gaattgaaga
     3601 ttcaacgcat cgataaccac aacttcactt tggcaaactt ggatgtgttt gaatgaatag
     3661 cgctctaatg cgggtgcgta tatcacacaa tctggaaatt gaatgagtag tgctgtgaca
     3721 ccaccgatat ggtcgtgatg gtgatgtgta attaaaatgc tatttaaggt taatccgag
//
LOCUS       DAOQSI010000180         3725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_27217_length_3725_cov_4.758583, whole genome shotgun sequence.
ACCESSION   DAOQSI010000180 DAOQSI010000000
VERSION     DAOQSI010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27217_length_3725_cov_4.758583"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3666
                     /locus_tag="PKL42_09445"
     CDS             <1..3666
                     /locus_tag="PKL42_09445"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HNU67338.1"
                     /translation="AAPEQTYLYAQDAKGGVLISENYYYNSEIYNTKLYATTDHPLYR
                     PGDTVNIKIVGREFINARDSKPLADAPIQVAVIDPNGLPVANQTLQFSGATGGDTAVK
                     LPVASVAGGYDIQMTYLGEVYSAAFRVAEFQKPHFEINLNVDDDDLKTGSVIPVQVQL
                     RYPDGKPVKNAEVDIDLKAQALSMVEGDLGYSGQFPIKLTSQKYQTDGSGHVKVNLPE
                     VKEPSRYIISTLATDGAAYRVRHTQELLIERAVSQWRIKANSRFTSPDQSVHFNVNQL
                     IESQTAQKNIETKSSQPTKWKWLRLEDRSSAEGEISQEFGKGFDIKFAKSGSYTVSLM
                     SKDGALLGATSHWVSGADLKVPVGHIEIVWDKANYQVGDNASALISFSEPIDHALVTL
                     ERENIDKASLLNAAASWLKTTRIAPQQWRISVAVTENFAPNLTLSVAYVKNNAFVFEN
                     AGILVSNAKVNVRVTADKKQYVPGEMVKLSIDTSLISADGKATNQAVSALVSLGVVDE
                     MLYVLQPEIAPNIVDYFYHPRRNNVRTQYSQSFIGYDLSTNQLGKSPSSHRAQERATK
                     VLERPRRDDVDTALWAPKITTDANGHAEVSFKMPDSLTRWRMTARAMTADGIVGQSVS
                     DIQSYKDFYIKWTSPKWLREKDSAIGNIAIFNQSNQDQNVKIEFQGALVQSESIRLKP
                     GINFIDVPRKGEQTGELILMLKHQDKIKDRLAVNLNNRSDSWPVHFNKLVSPQEGVIK
                     LDLPSDARNITLRWMDHSRAAFYRVVDDLVDQPYGCVEQTASRLIPLSLAYQSLLEDD
                     PRKTLMMRQLYTHRLRLASMAGPEAHFGWWGQDMAVDPFITTYAYYADWRTANALKLN
                     MPDEHWERLTKLYSESGFEQPVWQRALMLDWMTQIGLPTQSMITNLATDLAVATNATE
                     RKYGRHDSWVLTEESQQTRDLAVVLTAKLLKSSVEGFNAQISDSVERVKMHDSLVAKA
                     LLHYSGHELADVDVLLPLASADTATIDRSLMLTWVNGAKVPDPTSKGIPTPLAKPWLT
                     KSSSTHETFFVWPNSAPSTALPNTIASGNINAVVSYDSSAVAAQSTLPATLKRQLFLL
                     NKQEDGSFEPKLVDANDAVNTDALYLESLTLTPTKALRYVVIEAALPAGAQVESGTWG
                     IQIKDQSDMPKANFQDLADRYAVPVGYVDKEVVIQHLIRFSQRGQYVLPAARAYNMYS
                     PDAQVLESSPRKSIQVK"
BASE COUNT         1091 a          752 c          908 g          974 t
ORIGIN      
        1 gcagcccctg agcaaaccta tttatatgca caggatgcaa aaggtggtgt gctgatttcg
       61 gaaaactatt actacaatag cgaaatttat aataccaagt tatacgcaac aaccgaccac
      121 cctttatatc gcccagggga tacggtgaat atcaaaatag tgggccgtga atttatcaat
      181 gcgagagatt caaaaccgct agctgatgcg ccaattcaag tggcggtgat agatcctaac
      241 ggtttgccag tcgcgaatca aacattgcaa tttagcggtg caaccggagg agacactgct
      301 gtaaaactgc ctgttgcaag tgttgctggt ggctatgata ttcaaatgac ttatttgggt
      361 gaagtgtaca gcgcagcgtt tcgggttgca gagtttcaaa agccgcattt tgaaattaat
      421 ttaaatgtag atgatgatga cctaaaaaca ggcagcgtga ttcctgtgca agtgcaattg
      481 cgttacccag atggtaaacc cgttaaaaat gccgaggtag atattgactt aaaggcacaa
      541 gctttgagta tggtggaagg cgatttaggc tatagcgggc aatttccaat aaaattaacc
      601 agccaaaaat atcaaacgga tggcagtggt catgtgaaag tgaatctacc agaagttaaa
      661 gaaccaagcc gctatattat ctctactctg gcgacagatg gtgcagcata ccgcgttagg
      721 catacgcaag agctgctaat agaacgtgcg gtgagccaat ggcgcataaa agccaatagt
      781 cgatttactt caccagacca atcagttcac tttaatgtga atcagttaat tgaatcgcaa
      841 actgctcaaa aaaatataga aacaaagtca tcgcaaccca ctaagtggaa gtggctacga
      901 ttagaagata gaagcagtgc tgaaggagaa atcagccaag agtttggtaa agggtttgat
      961 attaagtttg caaaatctgg ttcctatact gtgagtttga tgagtaaaga tggggcttta
     1021 ctcggggcga ccagccattg ggtaagtggc gccgatttaa aagtacctgt ggggcatata
     1081 gaaattgtat gggacaaagc caactatcag gtaggcgaca acgccagcgc actcatttca
     1141 ttttcagagc cgattgacca tgcgctagtc actttagagc gagaaaatat tgataaagcc
     1201 agcctgttga atgccgctgc atcttggcta aagacaaccc gcattgcacc gcaacaatgg
     1261 cgcatttctg tggctgtaac tgagaatttt gcacctaacc tcacgctatc agtggcttat
     1321 gttaaaaata atgcatttgt atttgaaaat gcgggtattt tagtgtcaaa cgcaaaagtg
     1381 aatgtacgtg tcacagctga taaaaagcaa tatgtacctg gtgaaatggt taaattgtcg
     1441 attgatacct cattaatttc tgcggatggt aaagccacga atcaggcggt ttctgcactg
     1501 gtgagtttag gcgtagtgga tgaaatgcta tacgtcttac aacctgaaat tgcgcccaat
     1561 atcgtggatt atttctatca tccacgtcgc aataatgtcc gcacgcagta cagccagtca
     1621 tttattggtt acgatttatc taccaatcaa cttggcaaat caccaagtag tcatcgcgca
     1681 caagagcgag ctaccaaagt gttggagcgc ccacgacgtg acgatgtgga tactgcatta
     1741 tgggcgccaa aaattacgac agatgctaat ggccatgctg aagtcagttt taagatgcca
     1801 gattcgctca cgcgttggcg tatgactgca cgtgcaatga ccgcagatgg cattgtgggg
     1861 caaagtgtca gcgacattca gagttataag gatttttata ttaaatggac cagcccaaag
     1921 tggttgcgag aaaaagatag cgcgattggc aacattgcaa tttttaatca gtcgaatcaa
     1981 gaccaaaatg tgaaaattga gtttcagggt gcattggtac agagtgaaag tattcgttta
     2041 aaacccggca tcaattttat tgatgtgccg cgtaagggtg agcaaacggg tgagctcatt
     2101 ttaatgttaa aacaccaaga taaaattaaa gatcgtttag ctgtaaattt aaataatcgt
     2161 tcagacagtt ggccagtaca cttcaataaa cttgtgagtc cgcaagaagg ggtcattaaa
     2221 ctcgacttac catctgacgc acgcaatatt acgctgcgct ggatggatca ttcacgcgca
     2281 gcattttatc gcgtcgtcga tgatttagtt gatcagcctt atggttgtgt ggagcaaacg
     2341 gctagcagac ttatcccatt gtcgttggcc taccaatcat tgcttgaaga tgacccacga
     2401 aaaacattaa tgatgcggca gctctatacg caccgtttgc gtttggccag tatggccggg
     2461 cctgaggcac attttggctg gtgggggcaa gatatggcag ttgacccttt tattacgact
     2521 tatgcttact atgctgattg gcgtaccgcg aatgcactta aactcaatat gccagatgag
     2581 cattgggagc gattaacaaa gctatatagt gaaagtggct ttgagcaacc cgtttggcaa
     2641 cgtgcattga tgcttgattg gatgacgcaa attggcttgc ctacgcaatc gatgataacg
     2701 aatttggcga ctgatttggc tgtggctacc aatgccactg agcgtaagta tggccgtcat
     2761 gatagctggg tgctcactga agagagtcag caaacacggg atttagctgt tgtactcacc
     2821 gctaaattgc ttaagtcaag tgttgagggc tttaatgcgc aaattagtga ttcagtagag
     2881 cgggtgaaaa tgcacgatag tttggtggca aaagcgcttt tacattattc agggcatgag
     2941 ctggcagatg tggatgtgtt attgccactt gcgagtgctg atactgccac gattgaccgc
     3001 tcactcatgc tcacttgggt aaatggagca aaagtgcctg acccaacgtc taaaggcatt
     3061 ccgacaccgt tagctaagcc ttggctcact aaaagcagta gcactcacga gacgtttttt
     3121 gtttggccaa attcagcgcc atcaacagca ttaccaaata caattgccag tggcaatatc
     3181 aacgctgttg tgagttatga cagcagcgct gtggctgctc aaagtacgtt gcccgcaaca
     3241 ttaaagcggc agctattttt gttgaacaaa caagaagatg gcagttttga accaaagctt
     3301 gttgatgcca atgatgccgt caatacagat gcactttatt tagaaagcct cacgctcact
     3361 ccaactaagg cattacgtta tgtggtcatt gaagcagcgc ttccggctgg cgcgcaggtg
     3421 gagtcaggca cttggggcat tcaaattaaa gaccaaagtg atatgccaaa ggcgaatttt
     3481 caagacttgg ctgaccgtta cgcggtccct gtaggttatg tggacaagga agttgtgatt
     3541 cagcatttga tacgctttag ccaacgcggg caatatgtat tgcccgctgc gcgagcttac
     3601 aatatgtaca gcccagatgc gcaagtgctt gaaagtagcc cacgaaaaag cattcaggtt
     3661 aaatagtggg tcttatgcgt gctagttact ggcattggtt tgcaataatt acgctgacct
     3721 acagt
//
LOCUS       DAOQSI010000181         3679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_27716_length_3679_cov_3.838576, whole genome shotgun sequence.
ACCESSION   DAOQSI010000181 DAOQSI010000000
VERSION     DAOQSI010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27716_length_3679_cov_3.838576"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PKL42_09450"
     CDS             <1..226
                     /locus_tag="PKL42_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HNU67339.1"
                     /translation="SNPFIANDIPSPDESLVVIRFENPVGIDFPYLLSMLHDSMMTRP
                     NTIVVPGGKMGLAMQLIFTPMIMRLMSMRD"
     gene            254..1318
                     /gene="fba"
                     /locus_tag="PKL42_09455"
     CDS             254..1318
                     /gene="fba"
                     /locus_tag="PKL42_09455"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646216.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HNU67340.1"
                     /translation="MALVSLRQLLDHAAEHQYGLPAFNVNNMEQIHAIMQAADEVDSP
                     VILQASAGARGYAGEPFLRKLVEAAIEQYPHIPLCMHQDHGASPAVCQTALRSGFSSV
                     MMDGSLEADAKTPASFEYNVHVTQQVVEAAHAVGVSVEGELGCLGSLETGEAGDEDGG
                     GATGVLDKSQLLTDPDEAAAFVKATQVDALAIAIGTSHGAYKFSRPPTGDILAIDRIR
                     TIHARIPNTHLVMHGSSSVEQEWLQMIREYGGQIKETYGVPVSEIVEGIKNGVRKINI
                     DTDIRLAMTATIRKEMAHHPAEFDPRAFLKAATVAARSVCKNRFEAFGSAGQAAKIKV
                     IALDIMAARYTKGELAAIVR"
     gene            1338..2090
                     /gene="tpiA"
                     /locus_tag="PKL42_09460"
     CDS             1338..2090
                     /gene="tpiA"
                     /locus_tag="PKL42_09460"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNU67341.1"
                     /translation="MRRKLVIGNWKMNGSIAKNQLLLEAVIAGVQDLHHVDCVVCVPY
                     PYLGQAQALAQGTKVIWGAQNLCASEDGVLTGGVSPHMLVDYGCQYVIVGHSERRMLS
                     HETDDTAAARFIAAIKAGLTPIFCMGETKEERESDWTEYVVGRQLDSILRRFGPEVLA
                     KAVLAYEPLWAVGTNNPATPEQAQQVHKFIRHRIAKCDARVAKSVRILYGGSIQPSNA
                     AALFAMPDIDGGLVGRCSLDAQAFVAICEVAN"
     gene            complement(2290..>3679)
                     /gene="mqo"
                     /locus_tag="PKL42_09465"
     CDS             complement(2290..>3679)
                     /gene="mqo"
                     /locus_tag="PKL42_09465"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HNU67342.1"
                     /translation="NITMVEQLPEVALESSDALNNAGTGHAGYCELNYTPQANDGSIA
                     IQRALEINAAFEVSLQFWSNLVEQKILPTPSHFINKTPHLSLVWGEKNVAFLKHRFEL
                     LKQHPLFSDMQYSEDHAQLSRWMPLIMANREPKEKLAATFVAHGADVDFGALTRQLVG
                     YLKMQANFTLLSNTQVKKLKKIKNEWHVGLLNKASKHAKTINAKFVFLGAGGGALPLL
                     QLANVAESEGYGGFPVSGQWLICQNPAVIQQHNVKVYGKAAVGAPPMSVPHLDTRKIN
                     GKSALLFGPFAGFTTKFLKQGSALDLAKSLKVNNLKAMLGVGRHHMDLTKYLVKEAKQ
                     THEQRMDALREFLPQANSADWKLANAGQRVQIIKKCEKKWGKLEFGTEIVATQDGSLA
                     ALLGASPGASVSVQAMIHVIEQCFGTQLKHQVWNEKLKSLIPSYGQSLIVDSDLLRKV
                     RKHTLSTLQLTQ"
BASE COUNT          946 a          791 c          935 g         1007 t
ORIGIN      
        1 ttctaatcca tttattgcaa atgacattcc aagccctgat gaaagcttag tggtgattcg
       61 gtttgaaaat ccagttggga tcgattttcc atacttgctg agtatgttgc acgactcgat
      121 gatgacacga cccaatacga ttgtggtgcc aggcggaaaa atggggctgg cgatgcagtt
      181 gatttttacc cccatgatta tgcggctcat gagtatgcgc gactaatttt ttacaaatag
      241 caaaggcatc aacatggcgt tagtttcact taggcaattg ctcgatcacg cggcagagca
      301 ccaatatggt ctgcccgcgt ttaacgtgaa taatatggaa cagattcatg ccattatgca
      361 agcggctgat gaggtagata gccccgtaat attgcaggct tcggccggcg cgcgtggtta
      421 tgcgggtgag ccatttttac gtaagttggt tgaagcggcc attgagcaat acccgcatat
      481 tccattgtgt atgcaccaag accatggcgc ttcacccgcc gtgtgccaaa cggcactacg
      541 cagcggtttt tccagcgtga tgatggacgg ttcgcttgag gcggatgcta aaacgcctgc
      601 atcctttgaa tataacgtgc atgtgacaca gcaagtggta gaagcggcac atgcagtggg
      661 tgtttcagtg gagggtgaac ttggttgctt aggttcgcta gaaacaggcg aggcaggtga
      721 tgaagatggc ggtggcgcaa caggcgtatt agataagtcg caattactca ctgacccgga
      781 tgaagctgcc gctttcgtaa aagctaccca agtcgatgcg ctggcgattg ctattggtac
      841 tagccacggg gcgtataaat tcagccgtcc acccacaggg gacattttag ccattgacag
      901 aattcgtact atccatgcgc gtatccctaa cacgcatttg gtgatgcatg gctcatcaag
      961 cgtcgagcag gaatggctgc aaatgatacg cgaatacggc gggcagatta aagaaaccta
     1021 tggcgtgcct gtgtctgaaa ttgtagaggg cattaaaaac ggtgtacgaa aaatcaatat
     1081 tgatacggat atacgtttag ccatgacggc aacgattcgt aaagaaatgg cacatcatcc
     1141 tgctgaattt gatccgcgag cgtttttaaa agccgcgacg gtggccgcac gaagcgtgtg
     1201 taaaaatcgc tttgaagctt ttggtagcgc tgggcaggca gcaaaaatca aggttatcgc
     1261 gctggatatc atggctgccc gttacaccaa aggtgaactg gccgcgattg taagatagct
     1321 taaacgatag gtacataatg agacgtaaat tagtgatagg caattggaaa atgaatggca
     1381 gtattgccaa aaaccaattg ctacttgaag ctgtaatagc tggggtgcaa gatctgcatc
     1441 atgtggattg tgtggtgtgt gtgccatacc cataccttgg acaagcccaa gcgcttgcgc
     1501 aaggcaccaa agtgatttgg ggcgcgcaga atctgtgtgc cagtgaggat ggtgtgctga
     1561 caggtggtgt ttcgccgcac atgctggtag attacggctg ccaatatgtg attgtaggcc
     1621 actcggagcg ccgcatgtta tcgcatgaaa cggacgatac ggctgcggca agatttattg
     1681 ctgccattaa agctgggctc acgcctattt tttgcatggg tgaaaccaaa gaggaacgcg
     1741 agtccgattg gactgagtat gtggttggcc ggcaactgga ttcgatactc aggcgctttg
     1801 gcccggaggt tttggctaaa gcggttttgg catatgaacc cttgtgggct gttggtacaa
     1861 ataacccagc cacacctgaa caggcgcagc aagtacacaa atttatacgc caccgcattg
     1921 ccaaatgtga tgcgcgcgtg gcaaaaagcg tgcgtatttt gtatggcggc agcatacagc
     1981 catcgaacgc ggcggcctta tttgctatgc ctgatataga tggtggtttg gttggacgtt
     2041 gctcgttaga tgcacaggct tttgtggcaa tttgtgaggt agcaaattag tttgtttttg
     2101 gttcatcgat gaccactttt tgagcgattt taaactacaa cctttttgat tgctctgtaa
     2161 gccttataaa taaaggctcg cagggcagag attttctctc gaggggttta gtggcatatt
     2221 caaccattaa aatggtcatg caaaactagg gtgaacgcgc gggtagccac gttaaatttg
     2281 cagccaaatt tattgagtga gttgtaatgt acttaaagta tgcttacgca cttttcttag
     2341 caaatcgcta tccacaatta acgactgacc gtaactagga atcaatgatt ttaatttttc
     2401 attccatacc tgatgtttga gctgtgtgcc gaaacattgc tcaatgacgt gaatcatggc
     2461 ttgaacactc accgaagccc caggggaggc gccaagtaat gcggcgagtg agccgtcctg
     2521 cgtggcaacg atttctgtac caaattctag cttaccccat tttttttcac attttttaat
     2581 aatctgaacg cgttgtcccg cattggcaag cttccagtca gcagagtttg cttgcggtaa
     2641 aaattcacgc agtgcatcca tacgctgttc gtgcgtttgt ttggcttctt tcaccaaata
     2701 tttggtcaaa tccatgtgat gcctgccaac ccctagcatg gcttttaagt tatttacttt
     2761 caatgacttg gctaaatcta gggcagaacc ctgctttaga aatttagtgg taaatccagc
     2821 aaacgggcca aataacagtg cagatttccc gttgattttt cgcgtatcta aatggggtac
     2881 agacattggc ggtgcgccaa cggcggcttt gccataaact tttacattgt gctgctgaat
     2941 cactgctggg ttttggcaaa tcagccactg cccgctaaca ggaaagccac cataaccctc
     3001 actttctgca acgttagcaa gttgcagtag cggtagcgcg ccaccgcctg cacctaaaaa
     3061 gacaaatttt gcattaatgg tttttgcgtg tttgctggct ttattcaata aacctacatg
     3121 ccattcattt ttaatttttt tgagtttttt aacttgcgtg ttgctgagta aagtaaaatt
     3181 ggcctgcatt ttaaggtaac ccactagctg gcgggtgagg gcgccaaaat caacatcagc
     3241 accatgcgca acaaaggtag cggccaattt ctcttttggt tcacgattgg ccataatgag
     3301 tggcatccaa cggctgagtt gcgcgtgatc ttcactgtat tgcatgtcag aaaaaagggg
     3361 atgttgtttg agtaactcaa aacgatgctt taaaaaggcc acgttttttt cgccccacac
     3421 taaacttaaa tggggtgttt tgttaataaa atggctaggc gttggcaaaa ttttctgctc
     3481 aactaaattg gaccaaaatt gcagtgaaac ttcaaacgct gcgttgattt ctagggcgcg
     3541 ctgaatagca atgctgccat cattggcttg tggcgtgtaa ttaagctcac aatagcctgc
     3601 gtggcctgtg cctgcattat tcagcgcgtc agagctttca agcgccactt caggcagttg
     3661 ttcaaccatg gtgatgttg
//
LOCUS       DAOQSI010000182         3636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_28209_length_3636_cov_4.586149, whole genome shotgun sequence.
ACCESSION   DAOQSI010000182 DAOQSI010000000
VERSION     DAOQSI010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28209_length_3636_cov_4.586149"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..764
                     /gene="mnmD"
                     /locus_tag="PKL42_09470"
     CDS             45..764
                     /gene="mnmD"
                     /locus_tag="PKL42_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HNU67343.1"
                     /translation="MTHYSPAKLEWRDGQPYASAFQDVYFSSDNGLLETDYVFLQGNA
                     LQSRWQTLKTLHFTIAETGFGTGLNFLSVVKLWLEVAPATAMLHYISTEKHPLSLQDL
                     TSALELWPTLAEFSHHLLTNYEKLLQGNQALDLFQNRVRLSLLVGDATTQLRQIHEAV
                     DAWFLDGFSPAKNPDMWQTELFQQMARLSAVGSTFATFTSAGHVRRNLAAAGFNTQKR
                     AGFGKKREMLQGILMSKADAR"
     gene            754..1959
                     /gene="mnmC"
                     /locus_tag="PKL42_09475"
     CDS             754..1959
                     /gene="mnmC"
                     /locus_tag="PKL42_09475"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR03197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HNU67344.1"
                     /translation="MRVKTAIVIGGGIAGCSTAYALAQRGIKVSLVEQHENLGLEASG
                     NPCAMLYPRVSGNDALSSFALASFLYSLDFYQSLQLPATAFNTCGMLQTGYNSRELAR
                     ITKVAAFLNTNPSLKVASKLVNPIEASQIAGVSIDHAALYFSQAGWVNPSVLLARLTQ
                     HPHISIMTSCKIALIKNTGALWQVIDIQQHTLAADIVVIANANNAMHLLQSQHISTQP
                     VRGQVTLLEASADSRALNTILCSDGYFSPAMDGVHSLGATFDAGQSDLSIREADHQKN
                     LSALNTLSSALCQDLASRIQSGRASLRCVSTDYFPIVGKLIDADLLRQSPPRPNADIN
                     SLPWLDGLYINTGHGSKGFTSAPWCAELLAQRICGETASNDNVLLGAMNPNRFLLKRL
                     GLKRLAKMA"
     gene            1989..>3636
                     /locus_tag="PKL42_09480"
     CDS             1989..>3636
                     /locus_tag="PKL42_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNU67345.1"
                     /translation="MQTSTQETALKHYQQILLRNPKDIDAQIHCGNLCIELGRYEEAA
                     GYFRRLLRIFKTNQDVRDALCFALQALGNEAHTKHRFTMAEACFQEALHYQPKNAAYW
                     YNLGNAQRELGRPKEALKSFQESAKVNPNDADTLNNLGNVYRELGQLDQAILSYKQAL
                     EINPDLHHALAHLVHQQQHICDWQDLEHNINNVRQIVKSIPGARISPFAFLSMPGTTA
                     EEQKTCANHWVQNQFANLIALGEELNFKHQTLHSDKHQKIKIGYLSADFRLHPLAFLI
                     TELIELHDRTQFEVLAFSFGLDDKSSARIRLEKAFDAFHDIRFLTDIEAANKINACGI
                     DILVDLTGFTQTSRSGIAALKPAPINVNWLGFPGTMGAVAQRSNANNPLLQQPLFDYI
                     MTDDYITPPASAVHYAETLMPLPNCYQPNNHLRPIGKKPTRESCALSEGAFVFCCFNQ
                     SFKITPAIFAVWMRLLKARPNSELWLLECNVWAKQNLIIEAAKHGIASHRIIFAPRVS
                     IADHLARHVNADLFLDTLPYNAHTTCSDALWMGVPVLTCSG"
BASE COUNT         1063 a          865 c          779 g          929 t
ORIGIN      
        1 aagcgcgtta aaataagcaa aaaattgcgc gtattcaaca aaatatgact cactactccc
       61 ctgcaaaact tgagtggcgt gacggccaac catatgccag cgcatttcaa gatgtgtatt
      121 tttcaagcga caatggctta cttgaaaccg actacgtatt cttgcaaggc aatgcattac
      181 aaagccgctg gcaaacactt aaaacactgc actttacaat tgcagaaaca ggttttggta
      241 ctgggttaaa ttttctcagc gtagttaaat tatggctaga agttgcacct gcaacagcaa
      301 tgttgcatta catcagtact gaaaagcacc cattaagctt acaagaccta acctcagcac
      361 ttgaactttg gccgacgtta gctgaattta gccaccattt actgacaaat tatgagaaat
      421 tattacaagg taatcaggcg ctagaccttt ttcaaaaccg cgtgcgttta agtttgctag
      481 tgggtgatgc aaccacacaa ttacgccaaa tccatgaggc tgtcgatgct tggtttttag
      541 atggcttctc ccctgctaaa aatcctgaca tgtggcaaac cgaacttttt cagcaaatgg
      601 ccaggctgtc cgccgtcggc agcacgtttg ccactttcac cagcgctggc catgtgcgac
      661 gcaatcttgc tgcagcaggt tttaacaccc aaaagcgtgc cggctttgga aaaaaacggg
      721 aaatgctgca aggaatattg atgagcaaag ccgatgcgcg ttaaaaccgc cattgtgatt
      781 ggtgggggca ttgcaggctg cagcacggca tacgcgctgg cgcagcgcgg catcaaggtc
      841 agccttgttg agcaacacga aaacttaggc ttggaagcgt caggtaatcc atgtgcgatg
      901 ctctaccctc gcgtaagtgg caatgacgca cttagcagtt ttgcattagc gtcattttta
      961 tatagcctcg atttttatca atcattgcaa ttgcccgcca cggcattcaa tacttgcggc
     1021 atgctgcaaa caggttacaa cagcagagaa ctggcacgca ttactaaagt ggccgctttt
     1081 ttgaacacaa atccttcatt aaaggttgca tcaaaacttg ttaacccaat tgaagcgagc
     1141 caaatcgcgg gggtcagcat tgaccacgca gccctgtatt tttcgcaggc gggatgggta
     1201 aatcccagcg ttttgctggc acgattaacg caacatcccc atatttctat aatgacttca
     1261 tgcaaaatag cacttataaa aaatactggc gcgctatggc aggtcataga tatacaacaa
     1321 catacgcttg cagctgatat cgtagtaatt gccaacgcca acaatgccat gcatttactg
     1381 caaagccaac acatcagcac ccagccagtg cggggacaag tcacattgct tgaagctagt
     1441 gctgatagcc gcgcgcttaa cactatttta tgtagcgatg gctattttag ccctgccatg
     1501 gatggcgtac atagcttagg cgctactttc gatgccgggc aatccgattt gtcgattagg
     1561 gaagcagacc atcaaaaaaa cttaagtgcg ttgaacacgc tatcatcagc attgtgccaa
     1621 gatttagcat cgcgcattca atcaggccgt gcgagtttac gttgtgtcag caccgattat
     1681 tttccaatag taggaaagtt aattgatgcc gatttattga ggcaaagtcc acctcgccct
     1741 aatgcggata tcaacagttt accttggttg gatggcctct acattaacac cgggcatggc
     1801 agtaaaggct ttacaagcgc gccatggtgc gcagaattac tcgcgcaaag aatatgcgga
     1861 gaaacggcat ccaatgacaa tgtgttactg ggtgctatga atccgaatag gtttttactc
     1921 aaacgacttg gcctgaaacg cttagccaaa atggcctagc acgcgctaaa acacttacaa
     1981 taagcactat gcaaacttct acacaagaaa ccgcgttaaa gcattaccag caaatattat
     2041 tgcgtaatcc caaagacatc gacgcacaaa ttcattgcgg caacctttgt atagaattgg
     2101 gtcgttatga agaagctgcg ggctattttc gacgattgtt gcgcattttt aaaaccaacc
     2161 aagatgtgcg cgatgcccta tgttttgcgt tacaagcact aggtaacgaa gcacatacaa
     2221 aacaccgttt tacaatggca gaagcctgtt ttcaagaagc ccttcattat caacccaaaa
     2281 atgctgcgta ctggtacaac ctaggcaacg cgcaacgtga actaggccgc cccaaagagg
     2341 cgttaaaaag ctttcaagaa tcggcaaaag ttaaccccaa tgacgcagac acgctcaaca
     2401 acttgggcaa tgtgtatcgc gagcttggcc agcttgatca ggcgatattg agctataagc
     2461 aagccttaga aattaatccg gatttgcatc atgcgctggc gcatttggta catcaacaac
     2521 aacacatctg tgattggcaa gaccttgagc acaatatcaa taacgtgcgc caaatagtca
     2581 aaagcatccc cggcgcacga atttcgccct ttgcattttt atcgatgcct ggcacgacag
     2641 cagaagaaca aaaaacatgt gcaaatcatt gggtacaaaa tcagtttgcg aatttgatag
     2701 cacttggtga agaacttaat tttaaacatc aaactttgca tagcgataag catcaaaaaa
     2761 taaaaattgg ttacctttct gccgatttta gattgcaccc acttgctttt ctcattacag
     2821 aactcattga actgcatgat agaacccaat ttgaagtgct tgctttttca tttgggttgg
     2881 acgataaatc aagcgccaga atcaggttag agaaagcctt tgatgcgttt cacgatattc
     2941 gatttttaac agacattgaa gctgctaaca aaattaacgc ctgcggcatt gacattttag
     3001 ttgacttaac tggctttacc caaaccagcc gcagtggcat tgcagcgctc aagcctgcgc
     3061 caatcaatgt taattggctt ggctttcccg gcacgatggg ggcagtcgct caacgttcaa
     3121 atgcaaacaa tccattgcta caacaaccgc tgtttgatta catcatgaca gatgattaca
     3181 tcacaccacc tgcatcagcc gtgcattatg cagagactct gatgccattg ccaaactgct
     3241 atcagcccaa taaccaccta cgtccaatcg gcaaaaagcc gacgcgagaa tcttgcgcat
     3301 tgtctgaagg cgccttcgtc ttttgttgct ttaatcaaag ctttaaaatc acgccggcaa
     3361 tattcgccgt ttggatgcgc cttctaaaag caaggcctaa cagtgagctt tggttactgg
     3421 aatgtaacgt ttgggctaaa caaaatttga ttattgaggc ggctaaacac ggcattgcgt
     3481 cacacagaat catatttgca ccgcgcgtga gtattgccga ccatctagct cggcatgtaa
     3541 atgcagattt atttttagat accctaccct acaacgcaca taccacttgc agcgacgcct
     3601 tgtggatggg cgttcctgtt ttaacttgta gcggta
//
LOCUS       DAOQSI010000183         3633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_28249_length_3633_cov_4.493572, whole genome shotgun sequence.
ACCESSION   DAOQSI010000183 DAOQSI010000000
VERSION     DAOQSI010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28249_length_3633_cov_4.493572"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PKL42_09485"
     CDS             <1..534
                     /locus_tag="PKL42_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014002076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNU67346.1"
                     /translation="PVIKAGLAHLWFVTLHPFDDGNGRIGRAICDMALARADGSPQRF
                     YSLSAQMQREREDYYNSLEQTQKGTLDVTSWLAWFLGCLLRAVQSANEELKGVVYLAN
                     LSHHLPSGKLNERQMKMLNMMMHGFNGNLTTGKWAKIAQCSTDTALRDVSELVELGIL
                     IKAGESKRAAHYVLVSY"
     gene            complement(943..1044)
                     /locus_tag="PKL42_09490"
                     /pseudo
     CDS             complement(943..1044)
                     /locus_tag="PKL42_09490"
                     /inference="COORDINATES: protein motif:HMM:NF018933.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            complement(1246..1353)
                     /locus_tag="PKL42_09495"
     CDS             complement(1246..1353)
                     /locus_tag="PKL42_09495"
                     /inference="COORDINATES: protein motif:HMM:NF023742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNU67347.1"
                     /translation="MHHYTGCCRVLNNKALAWQNEQYQADNTSNSAIPR"
     gene            1565..2047
                     /locus_tag="PKL42_09500"
     CDS             1565..2047
                     /locus_tag="PKL42_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HNU67348.1"
                     /translation="MAVTDFGYKLIAKPNNSMSPRDTLMLVMGLATLSFVIAIAFARI
                     GAWLVLPFAGLEILAISYAFYYIHLHSDDFDSITIKQDEVVVEKRNDKQLTTTVFQRH
                     WAQVSVRDVASGGGAIGKRGLFIRSHGQEVEFGRNFINDEQRDVLALELKKKLKNIHS
                     "
     gene            2069..3241
                     /gene="coxB"
                     /locus_tag="PKL42_09505"
     CDS             2069..3241
                     /gene="coxB"
                     /locus_tag="PKL42_09505"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HNU67349.1"
                     /translation="MQFFKKIGVGLALMVASVSAFAEYAWNFPEPVTPMALDTLHVHN
                     KFMAICMIIFVVVLAIMVYSIFAHRKSKGYQAVADKPPSTAIEIFWTLIPFAILLLID
                     FVIMGIPAYHSVVMMEDTRTEAKMVVKITGSQWRWQYEYLDGDAKGIKFVSNLSTPQA
                     QMDKGYQGAKDENYLLEVDNRLVLPVGEKVRILMTASDVLHNWWVPQFGSSRVAVPGF
                     LRETWVQVEKPGTYRGQCKELCGKGHGYMPVVVDALPMDEYKVWVAAKKEEMAKASAG
                     ADKEWTKEDLMAEGKTVYEKNCAVCHQVSGAGLPPAFPALTGSKVATSPIFGEDGKYL
                     KDGHLDRVLNGIRVMPGWKALLNDVEIAAVVTYERNALGNSVGDVLQPSQVKTARE"
     gene            3303..>3633
                     /locus_tag="PKL42_09510"
     CDS             3303..>3633
                     /locus_tag="PKL42_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNU67350.1"
                     /translation="MSTTTTAHHDEHAEHPTGIMRWLTTTNHKDIGTMYLTFSLIMFF
                     VGGAMILGIRAELFKPGLQLINPDFFNQLIGVHALVMIFAALMPAATGFANWMIPLQI
                     GAPDMALP"
BASE COUNT          980 a          656 c          903 g         1094 t
ORIGIN      
        1 cccgttatca aggctgggtt agcacacttg tggtttgtta ctttgcaccc atttgatgac
       61 ggtaacggtc gtataggtcg cgctatatgt gatatggctt tagctcgcgc agatggctca
      121 ccacaaaggt tttatagctt atcagcacaa atgcagcgtg agcgtgagga ttactacaat
      181 agcttagagc aaacgcaaaa aggcacgtta gatgtcactt cttggcttgc gtggttttta
      241 ggttgtctat tacgcgcagt gcaaagcgca aatgaagagc tgaaaggcgt agtgtattta
      301 gctaatttaa gtcatcacct gcctagtggt aagttaaatg agcgtcaaat gaaaatgctg
      361 aatatgatga tgcacggttt taatggcaat ctcactacgg gtaaatgggc taagattgcg
      421 caatgctcta ccgataccgc tttgcgtgat gtgagtgagt tggttgaact aggcattctc
      481 ataaaagcag gtgaatcaaa acgagctgct cattatgttc tagtgagcta ttaatatatt
      541 ttgcatcgtg ttgaagttgg ctcctaaagg aagactttca gagatttaaa aggtttgaag
      601 tggataaaat aaggtggtgc taatatttag ctaaaaatga aatttagaaa atatttgtgg
      661 aaacttaaat tttaatcaag atgtttggta tcaaaatttg ccgttggcga agtatatttt
      721 tgacatcctc ccctcctgaa aacaggagat ttctacaggt ttccgcgatg atttgcagat
      781 accttcagcg ggttcgtgct tcacagtgcg gtctttttgc gccgtcactc cacacgctaa
      841 cgcggcattt cctgccgcta atatattgat tgcaccgacc aaatcagcat tttcagcata
      901 gacacattcc acacacttga acttagcttg ggtgaggcgg ttttatttgg caacgtgttt
      961 acaacacgga catgtgcgac tggtattgcg cggattaacc gctatcattt cgccgccggc
     1021 ccaggcctgc ttgtatttaa gctgtcgcct gaactcgaat cagccttggc attggatgga
     1081 gcgatttggc gagcatgcct ttcattttta aattgggaaa gtcggcacgt ttagcaaaga
     1141 cgtttctaaa actggactcc aggtttttaa gggactgttg cagggtttgg ctgggtgcgt
     1201 ctttgagcca aaccagctca tgtttccatt gtggcagtag ataggctatc ttggtatagc
     1261 tgaatttgaa gtgttatcag cttggtattg ttcgttttgc catgctaagg ctttgttatt
     1321 caacactcga caacagcctg tgtaatgatg catgacacag aatttttctc catttgacac
     1381 cagttaaaac ttgtaggctt ttctaaaaat attaacaaaa cgtagctgaa ttgttacaaa
     1441 tatgtaactt tttgcttgag cataattatt taatgtggta tttttttgat gtgcgtcaaa
     1501 ttgtcgcagt atcggatgag gtatgcgcgc tttttggaag tttctgggtt aaggaaattt
     1561 ttgtatggcg gtcactgatt ttggctataa attaattgct aagccaaata attctatgtc
     1621 acctcgtgac acgttgatgt tggtaatggg cttggcaaca ttaagcttcg taatagcgat
     1681 tgcttttgcc agaattggtg cttggctggt gttacccttt gctggattag aaattttggc
     1741 aattagttat gctttttatt acatccattt gcattcggat gattttgaca gcattacgat
     1801 aaaacaagat gaagtggttg ttgaaaaaag aaatgataaa cagctaacaa caacggtttt
     1861 tcaaaggcat tgggcgcaag tgagtgttcg cgatgtggca agtggtggtg gagcaatagg
     1921 taagcgcggg ctttttattc gttcgcacgg gcaggaagtg gagtttggtc gaaacttcat
     1981 taatgatgag cagcgtgacg tattggcgct agaactcaaa aaaaaattaa aaaatataca
     2041 ttcttaaatt tgtcttggag cggggtttat gcaatttttt aaaaaaattg gggtgggttt
     2101 agcattgatg gtggcatcgg tttctgcgtt tgctgaatat gcttggaatt tccctgaacc
     2161 tgtcaccccg atggcgcttg atacgttgca tgtgcataac aaattcatgg ccatctgtat
     2221 gattattttt gtggtggttt tggcgattat ggtttattcg atttttgccc atcgtaaaag
     2281 taaaggctat caagcggttg cagataaacc gccatcaact gcaatagaaa ttttctggac
     2341 actgattccg tttgcgattt tgcttttgat tgactttgtt attatgggta ttccagcgta
     2401 tcacagcgtg gtgatgatgg aagatacgcg taccgaagcg aagatggttg ttaaaattac
     2461 cggctcgcaa tggcgctggc agtatgagta tcttgatgga gacgcaaaag gcattaagtt
     2521 tgtaagtaac ttatccacac cgcaagcgca aatggataaa ggctatcaag gtgctaaaga
     2581 tgagaactac ctgctagaag tagacaaccg ccttgtatta cctgtaggtg aaaaagtacg
     2641 cattctcatg actgcttcag acgtgctgca caattggtgg gtgccgcaat ttggttcttc
     2701 acgcgtggca gttcctggtt ttctgcgtga aacttgggtg caggttgaaa agccaggtac
     2761 ctatcgtggc caatgtaagg agttgtgtgg caaaggccac ggttatatgc cagtggtggt
     2821 tgatgcgtta cccatggacg aatataaagt ttgggtagcc gctaaaaaag aagaaatggc
     2881 aaaagcctca gccggggctg ataaagagtg gaccaaagaa gacttgatgg cagaaggtaa
     2941 aaccgtctat gagaaaaact gtgcagtttg ccatcaggtg tctggtgccg gtttgccgcc
     3001 agcattccca gcgttaacag gtagcaaagt tgcaacaagc ccaatatttg gtgaagatgg
     3061 taaatatctt aaagacggtc accttgaccg tgtgttaaat ggcattcgtg tgatgccagg
     3121 ctggaaggcg ttgctaaatg atgttgaaat cgcggcagtg gttacttatg aaagaaatgc
     3181 gcttggcaac agtgtaggtg atgtgctaca gccatctcaa gtaaaaactg cacgtgaata
     3241 agtgaattaa ttagattcaa aataacaatt aaatattagg tttgagtgtt taggagaagg
     3301 caatgagtac aacaacaacg gcacatcatg atgagcacgc agaacatcca acaggcatta
     3361 tgcgttggct gaccaccacc aatcataaag atatcggtac gatgtacctg acgttctcgc
     3421 tgattatgtt ttttgtcggt ggtgccatga ttttaggcat tcgtgccgaa ttgtttaaac
     3481 caggcttgca gcttattaac cctgatttct ttaatcagtt gattggggtg catgcgttgg
     3541 tcatgatttt cgcggcgctg atgccggcgg ccaccggatt tgcgaactgg atgattcctc
     3601 tgcaaattgg cgcgccagat atggcattgc cac
//
LOCUS       DAOQSI010000184         3627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_28314_length_3627_cov_5.112542, whole genome shotgun sequence.
ACCESSION   DAOQSI010000184 DAOQSI010000000
VERSION     DAOQSI010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28314_length_3627_cov_5.112542"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1849
                     /locus_tag="PKL42_09515"
     CDS             <1..1849
                     /locus_tag="PKL42_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNU67351.1"
                     /translation="TKTPLFASSDAAPLLKGENGAFYRSNNDARGGNLAASYATENIS
                     VNYAGAFSKANNYTAGSNFKNGKATGRIGHTLGLDEVGSTAFETQNHTLGFAAKSGAH
                     LFEAKLGYQNMPEQLYPNQRMDLLDNEQKRINLRYAGDFDWGKLDARAYHEKVEHFMD
                     FGADKRFWYGGASGGNTGVSGTGNGRPCAPIGATCATGMPMYSEGKTTGATIKADIKL
                     AEKDLLRVGAELQQYRLDDWWTPSGAMMFPNTFTNINNGERDRTALFVEWEQHLNQQW
                     VTLAGVRYEHVKTDADNVTGYASTNMMGSNQLRDSAAFNAKNHSKTDNNWDLTALTRY
                     TKDANMDVEFGIARKVRSPNLYERYTWSTWQMAALMNNTVGDGNGYFGNVDLKPEKAH
                     TVSTTFDWHAADREWEFKATPFYTHVTDYIDAVQWNSTTNAARTALLKDQFTVLRYTN
                     QSARLYGLDLSGRMPLAQTRVGQFELKGLLNYTNGENRDTGDDLYNIMPLNTKLTLTH
                     NDAHWENALELHMVKSKDNVSDMRNEIKTPGYSLVNLRSSYSWKQARLDFGIDNLFDK
                     AYYLPLGGAYVGQGTTMTTALVPTGSVPLWGTAVPGMGRSVSVGLTLKY"
     gene            complement(1884..2867)
                     /locus_tag="PKL42_09520"
     CDS             complement(1884..2867)
                     /locus_tag="PKL42_09520"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNU67352.1"
                     /translation="MNTLTFPILRLLADGKFHSGEAIAQHFKVSRTTVWSAIQHAQTL
                     GIEIFSVRGRGYKLPNAIEFLNKDEVLRAIGEQRAWFNLEILDEVPSTNTYLMQQSAK
                     GAAHVTCIAAQVQTSGKGRRGRTWVSHLGASLTFSLLWRFQCGAAALSGLSLAVGVAL
                     IRALNHLGVQQAQLKWPNDVLVLDENKQGKKLAGILIELQGDMEGPSAAVIGVGVNLN
                     LPHHVLHAIDQPATDLAQHASINQSELLGVLLKHLADILSQFEQHGFTSLRDEWTRYH
                     AYHQQPVRMLMPDGRNVQGVVNNVAEDGILLVDTALGLQRFSAGEISLRSA"
     gene            3189..>3627
                     /locus_tag="PKL42_09525"
     CDS             3189..>3627
                     /locus_tag="PKL42_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HNU67353.1"
                     /translation="MSQEKTPIPAGSNFIRGIIERDLSENKFATKKWAGSPGDATHQK
                     TGQVDTAKIRTRFPPEPNGYLHIGHAKSIFLNFGLARDYGGICHLRFDDTNPEKESQE
                     YVDSITEMVSWLGFGWDNNGQNGKESNLYFASDYFDLMYRAAES"
BASE COUNT         1011 a          835 c          868 g          913 t
ORIGIN      
        1 tacaaaaacc ccactatttg catcatctga cgctgcccct ttacttaaag gcgagaatgg
       61 cgctttttat cgaagtaata acgacgcacg tggtggcaat cttgctgcca gctacgccac
      121 tgaaaacatc agcgtgaact atgcaggtgc gttttcaaaa gccaataatt atactgctgg
      181 gagtaatttt aaaaacggca aagcgacagg ccgtatcggc cacacattag ggcttgatga
      241 ggttggctct acggcctttg aaacacaaaa tcatacacta ggctttgctg caaaaagtgg
      301 tgcgcatttg tttgaagcca aacttggtta tcaaaacatg ccagagcagc tttatcctaa
      361 ccagcgcatg gatttgctgg ataacgagca aaaacgcatt aatctgcgtt atgcgggtga
      421 ttttgactgg ggcaagttag atgcccgtgc ttaccatgaa aaggttgagc actttatgga
      481 ctttggtgca gataaacgct tttggtatgg cggcgcatca ggtggcaata ctggcgttag
      541 cggcactggc aatgggcggc cttgtgcacc tatcggcgct acttgtgcga caggcatgcc
      601 catgtacagt gaaggtaaaa cgacaggcgc taccattaaa gccgacatca agctggcaga
      661 aaaagattta ttgcgcgttg gtgctgagtt acagcaatat cgtctggatg attggtggac
      721 gccatctggc gccatgatgt ttccaaacac atttaccaat attaacaatg gcgagcgaga
      781 ccgcactgca ctctttgttg agtgggaaca gcatttaaat caacaatggg tgaccttggc
      841 aggggtgcgt tatgagcatg taaaaacaga tgcagacaac gtaacaggct atgccagtac
      901 taatatgatg ggttcaaacc agttgcgaga tagcgcggcc tttaatgcca aaaaccacag
      961 taaaactgac aataattggg atttgactgc gctgacacgc tacacgaaag acgcaaacat
     1021 ggatgtcgag tttggcatcg cgcgtaaagt gcgctcaccc aacctgtacg aacgctacac
     1081 ttggtcaact tggcaaatgg cggcgctaat gaataacacg gtgggtgatg gtaatggcta
     1141 ttttggcaat gtggatttaa aaccagaaaa agcccacaca gtttctacaa cttttgactg
     1201 gcatgcagct gaccgcgaat gggagttcaa agcgacgcct ttctatacac atgtgacgga
     1261 ttatattgat gccgtgcagt ggaatagcac cacaaatgct gcccgcacag cgttgttgaa
     1321 agatcagttc actgttttgc gctacaccaa tcaatcggcg cgtctttatg gcttagattt
     1381 atctggccgt atgccgctgg cgcaaacgcg tgttggccaa tttgaattga aaggcttgct
     1441 caattacacc aatggcgaaa accgtgacac gggtgatgat ttgtacaata tcatgccgct
     1501 caacactaag ctgacattga cgcataatga tgcacattgg gaaaacgcct tagaactaca
     1561 catggtgaaa tcaaaagaca atgtttctga tatgcgtaat gaaattaaaa cgccaggcta
     1621 tagcttggtg aacttgcgtt cgagctatag ctggaagcaa gcgagactgg attttggtat
     1681 tgataacttg tttgataaag cctattacct acctttgggt ggcgcttatg ttggacaggg
     1741 tacaactatg acgactgcgc tggttccaac aggctcagtg ccactttggg gtaccgcagt
     1801 acctggcatg gggcgttcag tgagcgtggg cttgacctta aagtattagc aaaccgcaaa
     1861 agaggtgctt agtcattaat caattaagca cttcttaaac taatctcgcc cgcagaaaaa
     1921 cgctgtaggc cgagtgccgt atctactaac aaaataccgt cttctgctac gttattcacc
     1981 acgccttgca cattgcggcc atcgggcatg agcatgcgca ctggttgttg gtggtaagcg
     2041 tggtagcgtg tccattcgtc gcgtaatgaa gtaaaaccgt gctgctcaaa ctggctcaaa
     2101 atatcggcca aatgttttaa cagcacgccc agcaattcgc tttggttaat gctggcgtgt
     2161 tgcgctaaat ccgtcgccgg ttggtcaatt gcgtgcaaca catgatgcgg taaatttaaa
     2221 tttacgccta cgccaataac ggcggcactc gggccttcca tgtcgccttg cagctcaatc
     2281 aaaataccgg ccagtttttt gccttgttta ttttcgtcca gcaccagcac gtcgttgggc
     2341 catttgagtt gtgcttgctg cacgcctaaa tggttcagcg cgcgaatcag cgccacgccc
     2401 actgccaaac tcaagccaga aagtgctgcc gcaccacatt gaaagcgcca caataacgaa
     2461 aacgtcaggc ttgcacccaa atgtgaaacc cacgtgcgcc cgcgtcgccc tttgccgctg
     2521 gtttgcactt gcgcggcaat gcacgttaca tgcgccgcgc cctttgcgct ttgctgcatt
     2581 aaataggtgt tggttgaagg cacttcgtcc aaaatttcta aattaaacca agcgcgttgc
     2641 tcgccaatgg cgcgcaatac ctcatcttta tttaaaaatt ctatggcatt gggcagtttg
     2701 taaccacgcc cgcgcactga aaatatttcg attcctaacg tttgtgcgtg ctgaatcgcg
     2761 ctccaaacgg tagtgcgtga caccttaaaa tgctgcgcaa tcgcctcacc agagtgaaat
     2821 ttgccgtcgg ccagcaagcg taaaatggga aatgtcagcg tgttcataca agcgagagtt
     2881 tagcacagcc agttttagct tgcggtctcg tgaagttttg tgagtgaaat gggatgaaat
     2941 actaggctta aagtcagcct aaaaaatcat ttaaatacgc tcgttttacg cccgagcaaa
     3001 aaacattgcc atgagagcct ttttttataa ggctcacagg gcatcgaaaa aggttgtgct
     3061 taaaaattgg cattttaagc atttaaaatg gccgcttcaa acgcatgcaa tatgccgcat
     3121 aaaattgaca ctaaaccacc tgattgaata gtgtaaaata atgccaacct aaattttagc
     3181 gattacatat gtcacaagaa aaaacaccca tcccagcagg ctcaaacttt attcgcggca
     3241 ttattgaacg cgatttaagc gaaaataaat ttgccacaaa aaaatgggca ggcagcccgg
     3301 gcgatgctac gcaccaaaaa actgggcaag tggataccgc taaaatccgt acgcgttttc
     3361 cgcccgagcc gaatggctac ttgcatattg ggcacgctaa aagtattttt ttaaattttg
     3421 gtctcgcgcg cgattacggc ggcatttgcc atttacgctt tgacgacacc aacccagaaa
     3481 aagaaagcca agagtatgtg gatagcatca ccgagatggt gagttggctg ggctttggtt
     3541 gggataataa cggccaaaat ggcaaagaat ctaatttata ttttgcctct gattattttg
     3601 acttgatgta ccgcgcggca gaaagcc
//
LOCUS       DAOQSI010000185         3598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_28716_length_3598_cov_5.849845, whole genome shotgun sequence.
ACCESSION   DAOQSI010000185 DAOQSI010000000
VERSION     DAOQSI010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28716_length_3598_cov_5.849845"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..823)
                     /locus_tag="PKL42_09530"
     CDS             complement(149..823)
                     /locus_tag="PKL42_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6445 family protein"
                     /protein_id="HNU67354.1"
                     /translation="MDLYPSVTIIDNFYENPDEIRKFALNQKFTYCHELNNIDYVFPG
                     CRTKDLFEIDATLYEKVCKKLVSVFHNTKQDAMRWAISTSFQVVEAKFERGVLHQDGN
                     TIFAGVIYLTPNAPLNAGTSLFKPNSSFNEESYQAALKQSETNFKSNQPVSTEFHSMF
                     DETVRVNNVYNTLILFNAQTYHAANEFFGDSLATGRLAQVFFINKIDAKSYDIFPLQR
                     TKSIAI"
     gene            complement(993..2381)
                     /gene="ntrC"
                     /locus_tag="PKL42_09535"
     CDS             complement(993..2381)
                     /gene="ntrC"
                     /locus_tag="PKL42_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HNU67355.1"
                     /translation="MKPIWIIDDDKSIRWVFEKALTRTDLDYKTFSSVAEALNTLERE
                     QPQVVVSDIRMPNGSGLDFLTEIKQKYPDIPVIIMTAYSDLESAVAAFQGGAFEYLAK
                     PFDVDQAIDVIKRAVDESTREYTEKVTLEATPEIIGQAPAMQEVFRAIGRLSRSHATV
                     LINGESGSGKELVARALHRHSPRGDKPFIAINTAAIPKDLLESELFGHERGAFTGAAA
                     SRRGRFEQADTGTLFLDEIGDMPPDLQTRLLRVLSDGQFYRVGGHQPIKVNVRIIAAT
                     HQDLEERVKLGLFREDLFHRLNVIRLRLPALRERREDIPLLIKHFLQQSATELNVETK
                     QPSAAALKYLSAVNWSGNVRQLENVCHWLTVMAPGQNIDIADLPPELKEDNGKSNAST
                     TWQEALAAEVADALNRNEQHVLETRTQIFERILIEKALEHTHGRRIEAANQLGMGRNT
                     ITRKIQELGIEE"
     gene            complement(2426..3520)
                     /gene="glnL"
                     /locus_tag="PKL42_09540"
     CDS             complement(2426..3520)
                     /gene="glnL"
                     /locus_tag="PKL42_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HNU67356.1"
                     /translation="MVQSTPNLFNGLEHLTTAIILLNKNCHVVYINPAAEIIFAFSAT
                     HVTGMPISEVFPDCEILMLAIKNAIAQQSPFREHEFTITTNRFQSFSVTCTATPIDAP
                     NTCLILEFQQMDAQLRIAREERMLIQQQANAELLRNLAHEIRNPLGGLRGAAQLLEHE
                     LPSPSLREYTQVIIKEADRLQTLMDRLLIPHRAPKYQPTNIHEVLERVRSLLLAESPK
                     AILIQRDYDLSLPELIGDREKLIQAVLNIARNAAQAMQAHNTPDSKIIFRTRAERQVT
                     LARKRYRVAIKLEIIDNGPGIPADIRDRIFYPLVSGAEGGTGLGLTLAQTFITQHHGM
                     IECESEAGRTCFTILLPIETSAVKPAATMQ"
BASE COUNT          926 a          761 c          857 g         1054 t
ORIGIN      
        1 gatttaaaaa tcgctcctac agactatggt ttttttgatt aaataaacca aaaatccccg
       61 caaccttttt tgatgccctg cgagccttta tttataaggc tcgcagggca tatatttttt
      121 ctcgggggat ttgttgaatt aaattgcttt aaatagcgat ggattttgtg cgttgtaatg
      181 gaaaaatatc gtagctttta gcgtcaattt tgttgataaa aaagacttgc gctaacctgc
      241 ctgttgcgag ggagtcacca aaaaattcat tcgccgcgtg gtaggtttgc gcgttaaata
      301 aaataagcgt gttgtaaacg ttattcaccc tgacggtttc atcaaacatg ctatgaaact
      361 cagttgaaac gggctggttg gatttaaagt tggtttcaga ttgcttgagc gcggcttggt
      421 agctttcttc attaaagctg gaatttggct taaataacga tgtgccagca ttaagtggcg
      481 cattgggtgt taagtaaatt acaccggcaa aaatagtgtt gccatcttga tgcagcacgc
      541 cacgctcaaa tttagcttca acgacttgaa agctggtgga aattgcccaa cgcattgcgt
      601 cttgttttgt gttgtgaaaa acagagacta attttttaca gactttttcg tataaagttg
      661 catcaatttc aaacaaatct tttgttctgc aacccgggaa tacgtaatca atattattta
      721 attcatgaca ataggtaaat ttttgattaa gcgcaaactt acgaatttca tcggggtttt
      781 cataaaagtt atcaatgatc gtgacgcttg ggtacaaatc catagggtga actttcaaat
      841 gactattagc cgaattcgct gattctacca tgtgaagatt catacaaaca aactgagaat
      901 gacgttgatt acatctgcac tgctctgcga gcctttattt ataaggcttg cagggcagcg
      961 ttttttgctc gggcaatggt tagcgtaata atttattctt caatgcctaa ttcttgaatt
     1021 tttctggtaa tggtattgcg gcccatgccg agctggttgg cggcttcaat tcggcggcca
     1081 tgcgtgtgtt cgagcgcttt ttcaataaga atgcgctcaa aaatctgggt gcgggtttca
     1141 agcacatgtt gttcattgcg gtttagtgcg tctgccactt cagcggcaag cgcctcttgc
     1201 caggtggtgg aagcatttga ttttccatta tcctctttaa gttcgggtgg caaatctgca
     1261 atatcaatat tttggcccgg tgccatcacc gtgagccagt ggcaaacgtt ttcaagctgc
     1321 cgtacattgc cgctccaatt caccgcgcta aggtatttga gcgcagcagc tgagggttgt
     1381 ttggtttcaa cattcaattc agtggcgctt tgttgcagaa aatgcttaat gagtaaggga
     1441 atatcttcac ggcgttcgcg tagcgcgggt aagcgtaggc gaataacatt gaggcggtgg
     1501 aataagtctt cacgaaaaag cccgagttta acgcgttctt caaggtcttg gtgcgtggcg
     1561 gcaataatgc gcacattcac tttaatcggc tggtggccgc ccacacggta aaattggccg
     1621 tcacttaaca cgcgtagcag gcgtgtttgt aaatctggcg gcatgtcgcc aatttcatct
     1681 aaaaagagtg tgccggtatc agcttgctca aagcgccccc gacgcgatgc cgccgcgcct
     1741 gtaaatgcac cacgctcatg gccaaacagt tcagattcta ataaatcttt aggaattgct
     1801 gcggtgttaa tggcaataaa gggcttgtct ccacgcgggc tatggcgatg caacgcgcgc
     1861 gccacaagct ctttaccgct gccagattca ccattgatga gcaccgtggc gtgcgagcga
     1921 cttaagcggc caatggcgcg aaaaacctct tgcatggcag gtgcttggcc aataatttca
     1981 ggggttgcct ccagcgtgac cttttcagtg tattctctcg tgctttcatc aaccgcgcgt
     2041 ttaatgacat caatcgcttg gtcaacatca aagggtttgg ctagatactc aaaagcgcct
     2101 ccttgaaaag ccgctacagc actttcaaga tcagaatagg ctgtcatgat aatgaccggg
     2161 atatcaggat atttttgctt aatttcagtg agaaaatcta agccagaccc attgggcata
     2221 cgaatatcac tcaccacgac ttgcggctgt tcccgttcca gcgtattaag cgcctctgcc
     2281 accgatgaaa aagtcttata gtctaaatca gttcgtgtga gggctttttc aaatacccaa
     2341 cgtattgatt tgtcatcatc gataatccaa atgggtttca tatttgttaa tccttaaggg
     2401 tatttcttgt ttgttggctc gtgttttatt gcatcgttgc ggctggtttt accgcactag
     2461 tctctatggg taataaaatc gtaaagcaag ttctacctgc ttcgctttca cactcaatca
     2521 tgccgtgatg ctgagtaata aaagtttgtg caagtgttaa gcctagccct gtgccgcctt
     2581 ctgcacccga tacaagcggg tagaaaatac ggtcgcgtat gtcggctgga atgccgggcc
     2641 cgttatcaat aatttccagt ttaattgcca cgcggtaacg cttgcgtgca agggtcactt
     2701 gccgttcagc acgcgttcta aaaataattt tactgtcagg tgtgttgtgc gcttgcatgg
     2761 cctgagcggc attacgggca atattcagca cggcttggat gagcttttct cggtcaccaa
     2821 tgagttctgg taaacttaaa tcgtagtcgc gttgtatcag aatcgccttt ggcgactcgg
     2881 cgagcagtaa gctacgcaca cgttcaagca cttcatgaat gttagtcggc tgatattttg
     2941 gtgcgcgatg tggaatcaac aggcgatcca tcaaggtttg caggcgatca gcctctttaa
     3001 taatcacttg cgtgtattcg cgtaaacttg gtgaaggtaa ctcatgctca agcaattgcg
     3061 ccgcgccacg caggccacca agcggattgc gaatttcatg ggcgagatta cgcaatagtt
     3121 cggcattggc ttgttgctga atgagcatgc gctcttcacg cgcaatgcga agttgcgcgt
     3181 ccatttgctg aaattctaaa ataaggcacg tatttggtgc atcaatcggt gtggctgtac
     3241 aggtgacaga aaatgactga aaccggttag tggtaatggt gaactcatgt tctctgaaag
     3301 gactttgctg tgcaatggca tttttaatgg ccaacatcaa tatttcacaa tcaggaaata
     3361 cctcgctaat tggcatgcca gtgacatgcg tggcgctaaa agcaaagata atttcggccg
     3421 ctgggttgat gtagaccaca tggcaatttt tgttgagcag aataatcgca gtggtgagat
     3481 gctctagccc gttaaacaaa tttggggtgg attgaaccat tatttagtta ttgcaccgaa
     3541 ataaaacgaa gggttgttaa gtcatgaatc atcatactat gattaaagca aaaatgag
//
LOCUS       DAOQSI010000186         3553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_29274_length_3553_cov_3.919954, whole genome shotgun sequence.
ACCESSION   DAOQSI010000186 DAOQSI010000000
VERSION     DAOQSI010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29274_length_3553_cov_3.919954"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="PKL42_09545"
     CDS             complement(<1..722)
                     /locus_tag="PKL42_09545"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNU67357.1"
                     /translation="MENIRYDLITALPEIVVLSMAMLILLADLFLNKSNRIVIYWLAQ
                     ATLFVAAYFTASTHTSSVGYAFNGMFVDDSLADVLKLMIYLGTSLIFVYSRQYIVDRN
                     MYRGEFYALVLFAMVGMMVMVSGQNMLTLYIGLELLSLSLYALVALDRDNARATEAAM
                     KYFVLGALASGMLLYGMSMIYGMTASLNISDISHALMNAPAPHKELYAVLILGLVFIV
                     AGLAFKLGAVPFQMWVPDVYEGS"
     gene            complement(726..2252)
                     /locus_tag="PKL42_09550"
     CDS             complement(726..2252)
                     /locus_tag="PKL42_09550"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNU67358.1"
                     /translation="MLELFTLDYFYRHLLSFAIWLPIAAGIAVLFTANDNKPNITRWL
                     ALLGSVLAFLVTIPLYTQFNFADGGFQFQEKFSWISSFNIHYHLGADGIAVPLILLTS
                     FTTVIVVLAGWEVIEKRVAQYMASFLIMSGLMIGVFSAIDAILYYVFWEAMLIPMFLV
                     IGIWGGPNRVYATIKFFLYTLLGSLLMLVAFIFLFYQTGSFAITDYYLLAMPLNVQVL
                     IFLAFFAAFAVKIPMWPVHTWLPDAHVEAPTGGSVVLAAIALKLGGYSFLRFAMPIAP
                     DAAHYLSGYMIALSLIAIVYIALVALVQRDMKKLIAYSSISHMGFVTLGFFIFNNLGL
                     EGAIVQMISHGFISAAMFLCVGVLYDRVHSRQIESYGGVANTMPMFAAFAVLFAMANS
                     GLPGTSGFVGEFMVILGAIQVNFWFAFLASFTLIFGAAYTLWMVKRVFYGEVANEKVA
                     ALKDLNKREFLILAILAILVLALGIYPQPLTELTNATVTQLLSHLSQSKLPIGLSAGL
                     "
     gene            complement(2255..>3553)
                     /locus_tag="PKL42_09555"
     CDS             complement(2255..>3553)
                     /locus_tag="PKL42_09555"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNU67359.1"
                     /translation="AQVPLHVWLPDSMEGPTPISALIHAATMVTAGIFMVARMSPLFE
                     LSTTALSFVLIIGSITALFMGFLGIIQNDIKRVVAYSTLSQLGYMTVALGVSAYSVAI
                     FHLMTHAFFKALLFLGAGAVIMGMHHDQDIRNMGNLKKYMPVTWITSLIGSLALIGTP
                     FLSGFYSKDSIIEAAKASTIFGSGFAYFAVLAGVFVTAFYSFRMYFLVFHGEEKWRSV
                     QHDAHHNDHDHHHGLAPTDDPHEPGWVVKLPLVLLAIPSLAIGYIAIEPMLFGDFFKG
                     VITVDSAAHPAMKALTHHWHTIYHSATGMALHSFTTLPFILAASGVFTAWFFYMKRPD
                     IPALIQTKFAVINTILEKKYGFDDFNERVFAFGSRYIGEKLWQVGDVKIIDGGMVNGT
                     ANLIGRLSGVVRKLQTGLIYHYAFAMIIGVFLLLTFFTKV"
BASE COUNT         1220 a          828 c          678 g          827 t
ORIGIN      
        1 gatccttcgt aaacatccgg cacccacatt tgaaaaggta ctgcgcccaa tttaaaagcc
       61 aaccctgcca caataaaaac caagcccaaa attaacactg catataattc tttatggggt
      121 gctggggcat tcatgagtgc gtgactaatg tctgaaatat tcaaactggc agtcatgccg
      181 taaatcatac tcataccata gagcagcatg cctgaagcta aagcgcccag tacaaagtat
      241 ttcattgcgg cctcggtggc tctggcatta tcacgatcta gcgcaacaag cgcatacaaa
      301 cttagtgaca ataactctag accaatatat aaagtcagca tattctggcc tgaaaccatc
      361 accatcatac caaccattgc aaacaggacg agcgcataga actcaccgcg atacatattg
      421 cggtcaacaa tatactgccg gctataaacg aaaataagtg aggtgccaag ataaatcatg
      481 agcttcagca catctgctaa agagtcatcc acgaacatgc cattaaaagc atagccaaca
      541 cttgatgtgt gcgtgcttgc tgtgaaataa gccgcaacaa acaaagtggc ttgcgctaac
      601 cagtaaataa cgatgcggtt acttttgttt aaaaataaat ctgccagcaa tatcagcatc
      661 gccatactca acactactat ctcaggtaat gctgtgatta aatcgtaacg aatattttcc
      721 ataacttata gccctgctga caaaccaatg ggcaacttgc tttgcgatag atgagaaaga
      781 agttgtgtca cagtagcatt agtgagttca gtcaatggtt gtgggtaaat accaagtgct
      841 agcaccaaaa tggctaaaat agccaaaatc aagaattcgc gcttattcag atcctttaat
      901 gcagctactt tttcattggc gacttcaccg taaaacaccc gctttaccat ccataaagta
      961 taggcagccc caaaaatcaa ggtaaaagat gctaaaaatg caaaccaaaa attcacttgt
     1021 attgccccta atatcaccat aaactcaccc acaaaacctg aggttccggg taacccacta
     1081 ttggccatcg caaataaaac agcaaaagca gcaaacattg gcatggtgtt agcaacaccc
     1141 ccgtaggatt caatttggcg actatgtact cggtcataca agacaccaac acataggaac
     1201 atcgctgcag agataaaccc gtgcgatatc atctgaacga ttgcgccctc taaccctaaa
     1261 ttattgaaaa tgaaaaaacc taatgtgaca aaacccatat gagagattga tgaataagca
     1321 attaattttt tcatatctct ttgcacaagt gcgactagcg caatgtacac aatggcaatt
     1381 aaagataacg caatcatgta acctgataaa taatgtgctg catctggagc tattggcatc
     1441 gcaaagcgta agaaactata accaccaagc ttaagtgcaa ttgcagccaa caccacagag
     1501 ccacccgttg gcgcctcaac gtgcgcatcc ggcaaccagg tatgtactgg ccacattgga
     1561 atcttcacag caaaagcagc aaaaaatgcg agaaaaatta acacctgcac atttaacggc
     1621 atggcaagca aatagtaatc tgtaattgca aaactgcccg tttggtagaa aagaaatata
     1681 aacgcaacca acatcagcaa tgagcctaga agcgtgtaca gaaaaaactt aatcgtagcg
     1741 tatacgcgat taggccctcc ccaaatacca atcaccaaaa acatggggat tagcatcgcc
     1801 tcccaaaata cgtaatacaa aattgcatcg atggcactaa acacgcctat catcaaaccg
     1861 ctcatgataa gaaaggatgc catatactgc gcgacacgct tttcgatgac ttcccaacca
     1921 gccagcacaa caatcaccgt tgtaaagcta gtcaatagaa tcaagggtac tgcaatgcca
     1981 tcagcgccta aatggtaatg aatattaaac gacgaaatcc agctaaactt ttcttgaaat
     2041 tgaaagccgc catcagcaaa attaaactgc gtataaagcg gaatggtgac taaaaacgca
     2101 aggacactac ccaacaaagc aagccaacgc gtgatattgg gcttattatc attcgcagta
     2161 aaaagcacag caataccagc ggcaatcggc aaccaaatgg cgaaacttaa aagatgtcga
     2221 tagaaataat ccaaagtgaa gagttcgagc atgtttagac tttagtgaaa aatgtgagca
     2281 atagaaacac gccaatgatc atggcaaatg catagtgata aatcagtcct gtttgtagtt
     2341 tgcgcactac acctgacagc ctgccaatta aattggcagt accattgacc atcccgccat
     2401 cgataatttt gacgtcgccc acttgccaaa gcttttctcc aatatagcga gagccaaatg
     2461 caaagactcg ctcattaaaa tcatcaaaac catatttttt ttccagtatg gtgttaatga
     2521 cagcaaattt agtttgaatc aatgcaggta tatcagggcg tttcatatag aaaaaccaag
     2581 ctgtgaacac tccagacgct gccaagataa acggcagggt tgtaaagcta tgtagcgcca
     2641 tacctgtagc ggaatgataa atcgtatgcc agtgatgcgt taacgctttc attgctggat
     2701 gtgctgccga atctactgta atcacaccct taaaaaaatc accgaacagc attggttcaa
     2761 ttgcaatata gcctattgct aatgagggta tcgccaataa aaccaggggc aacttcacaa
     2821 cccagccagg ctcatgagga tcatccgtgg gcgctaggcc atggtgatgg tcgtggtcat
     2881 tatggtgtgc atcatgctga acgcttcgcc acttttcttc accatgaaac actaagaaat
     2941 acatgcgaaa actatagaag gccgttacaa atacgcctgc gagaacagca aaataggcaa
     3001 acccacttcc aaagatagta gaagcttttg ctgcttcaat aatgctgtct tttgaataaa
     3061 agcctgataa gaatggcgta cctattaggg cgagtgaacc aattagcgag gtaatccaag
     3121 taactggcat gtattttttt aagttaccca tattacgtat atcttggtca tgatgcatgc
     3181 ccatgatgac agcaccggca cctaaaaata acagtgcttt aaagaatgca tgtgtcatca
     3241 agtgaaatat cgccacagaa tacgctgaca cacccaaagc aaccgtcatg taacctaatt
     3301 gcgacaaggt ggaatatgca accacgcgct tgatatcatt ttggataatt cccaaaaagc
     3361 ccataaacaa tgcggtaata ctaccgataa tcaacacaaa cgaaagtgcc gttgtagata
     3421 actcgaataa aggtgacatt ctagccacca taaatatccc tgctgtcacc attgttgctg
     3481 cgtgaatcaa ggccgaaata ggtgtagggc cctccatcga atctggcagc cacacgtgca
     3541 atggcacttg tgc
//
LOCUS       DAOQSI010000187         3526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_29594_length_3526_cov_5.707001, whole genome shotgun sequence.
ACCESSION   DAOQSI010000187 DAOQSI010000000
VERSION     DAOQSI010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29594_length_3526_cov_5.707001"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="PKL42_09560"
     CDS             complement(<1..622)
                     /locus_tag="PKL42_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNU67360.1"
                     /translation="MKVLVLGSGVVGVTSAYYLAKKGFEVTVIDRQPAVALETSFANA
                     GQISPGYSAPWAAPGVPAKALKWLFQRHSPLAIKPDGTLWQLQWVLQLLQNCNAKSYA
                     RNKERMVRLAEYSRDCLIQLRAELDIQYEGRAQGTLQVFRTQQQLNAEAKDIAVLTRM
                     GVPFEHLDPDGCVRAEPALALVKNQLLGGLRLPGDETGDCQLFTTQL"
     gene            complement(811..1926)
                     /locus_tag="PKL42_09565"
     CDS             complement(811..1926)
                     /locus_tag="PKL42_09565"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNU67361.1"
                     /translation="MKNKNSLVIALLGALLSMSFAGISVAKEEEKVLNVYNWIDYIAP
                     ETIKNFEKETGIQVRYDTYDGNEVLDEKLRSKKSGYDIVVPSSDWAKAQINSGLLQKL
                     DKSKIPNFKNLDPDILVKLNKADPGNAYLAGWLWGYTTIGLDVDKVIQALGKTPMPKN
                     LWELVFNPTYTTKLKSCGIMYLDSPTDIMPIALNYIGKPAFSNNPADYATATAMLKKV
                     RADIKTITSEGQAEKLGDQSICVAIGWGGDFYRARSEAIKKGNGLNIAPVFPEKGALL
                     FLDSMAIPKDAKHPNNAHQFINYILRPEVHAGITNATKYANPNKASTPLVNVEMRNDR
                     SLFLAPEDFSRLTAPETLDGNLINVRAEAFEKFKAAK"
     gene            complement(2179..2736)
                     /locus_tag="PKL42_09570"
     CDS             complement(2179..2736)
                     /locus_tag="PKL42_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNU67362.1"
                     /translation="MIEQDLGARLRMVRLRHQLSQRALAKKAGVANATISLIESGQTS
                     PSVSALKRILAGVPMTLAEFFSDELPQVKSEVFYRAEELTEISAGAGISYRQIGSAKA
                     GHVLQILYETYAVGADTGLVMLQHEGEEGAVILSGKLEVTVGNLTRVLGAGDAYYFNS
                     QTPHRFRNVGKTPCILVSSCSPPTF"
     gene            2909..>3526
                     /locus_tag="PKL42_09575"
     CDS             2909..>3526
                     /locus_tag="PKL42_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNU67363.1"
                     /translation="MSKASTNRLPDLSHYWVPFTANRQFKTMPRLFKAARGNYYTTMD
                     DRQVLDGTAGLWCVPAGHGREEIASAVGKQLLTLDYAPAFQSSHPLAFEAASRVADYM
                     PSGLDRIFFTNSGSESADTALKIALAYHRACGNPLRNKLIGRERGYHGVNFGGIAVGG
                     IAGNRKAFGNALSGVDHIRHPLDIAKNAFTKGVPQDGGIALANEFE"
BASE COUNT          959 a          824 c          742 g         1001 t
ORIGIN      
        1 ccaattgagt ggtaaaaagc tggcaatcac cggtttcatc gcccggcaag cgtaatccac
       61 ccagtaactg attttttacc aatgccaaag caggctcggc acgcacacag ccatctgggt
      121 caaggtgctc aaacggcacg cccatacgcg ttagcacggc aatatctttt gcttcagcgt
      181 tgagttgttg ttgcgtgcga aacacctgca aagtgccctg cgcacggcct tcatactgaa
      241 tgtctaactc tgcacgaagc tgaattaagc aatcacggct atattcggcc aagcgcacca
      301 tgcgctcttt attgcgggca taacttttgg cgttgcaatt ttgtaaaagt tgaagcaccc
      361 attgcaactg ccataacgtg ccatctggct taatggccaa gggtgaatgg cgctgaaaca
      421 gccattttaa ggctttggct ggcacaccgg gcgccgccca tggcgctgaa taacctggtg
      481 aaatctgccc ggcattggca aaactggttt ctagtgcgac ggctggctgg cggtcaatga
      541 ccgtgacctc aaaccctttt ttggctaaat aatatgcact agtaacaccc accacacccg
      601 aacctaatac cagcactttc atcgcaaata ctcagtttat gacaatgatt ctagtgtaga
      661 acgaattaaa atttaatcaa ggcaaaaagc aagaaatcat gtcaaatttc gcccgagaaa
      721 aaaataatgc tctgagagcc tttatttaca aggctctcag agcatcgaaa aaggttggtg
      781 cagattttat gctgttaatt tgctaataaa tcattttgcc gctttaaatt tttcaaacgc
      841 ttccgccctc acgttgatga gattgccatc gagcgtttct ggcgcagtga ggcgactaaa
      901 atcttcaggg gctaaaaata gcgaacgatc attgcgcatt tcaacattga ctaaaggtgt
      961 tgaagccttg ttcgggttgg catattttgt ggcattggta atgcccgcgt gcacttcagg
     1021 acgaagaata taattaataa attgatgggc attattggga tgtttggcat ctttaggaat
     1081 cgccatgcta tctaaaaaca acaatgcgcc tttttcagga aacactggcg caatgtttaa
     1141 gccattgcct tttttgatgg cttcgctgcg tgcgcgataa aaatcaccac cccagccaat
     1201 cgccacgcaa atggattgat ccccaagttt ttccgcttga ccttctgatg taatggtttt
     1261 gatatcagcg cgcacttttt ttaacatagc cgttgccgtt gcgtaatctg cggggttatt
     1321 actaaaggct ggcttgccaa tatagttaag tgcaattggc ataatgtcag tgggcgaatc
     1381 caaatacata atgccgcagc tttttagttt agtggtgtaa gttggattaa aaacaagctc
     1441 ccatagattt ttcggcattg gcgttttacc caaagcttga atcactttat ccacgtccag
     1501 acctatggtc gtgtaacccc ataaccagcc tgccagataa gcattgccag ggtcagcttt
     1561 attcagcttc actaaaatat ccgggtcaag atttttaaaa tttggaattt tacttttatc
     1621 tagcttttgt agtaaaccgc tgttaatttg cgctttagcc caatctgatg atggaacaac
     1681 aatatcatag ccggattttt tagaacgtaa tttttcatcg agcacttcat tgccatcgta
     1741 agtgtcataa cgcacttgaa tgccggtttc tttctcaaag tttttaatgg tttctggcgc
     1801 aatgtaatca atccagttat acacattcaa tactttctct tcctcttttg ccacgctgat
     1861 accggcaaaa ctcatactta atagcgcacc tagcaatgca ataaccagac tatttttatt
     1921 tttcacgttt gtttccttca attatttttg atattttttt gctttccagg gctgatgtta
     1981 tgcaagtgtt atgccattgt gttggatttt ttatactgaa tcatggtcag ccggcaaaaa
     2041 acccgattca tgggcaattt ttaaattgca gtttcaaaaa gtaatggtaa gtccattgcg
     2101 agtgaatgtt taaaaagtga cactgtttgt cataaatttg tcattaaact taaaatgcaa
     2161 agacgttttc attacaaatt aaaacgtcgg cgggctgcac gaactcacta aaatgcaagg
     2221 cgttttcccc acattgcgaa agcggtgcgg tgtttggcta ttaaaataat aagcatctcc
     2281 cgcgcccagc acgcgcgtga gattacccac cgtgacttct aacttacctg ataggatcac
     2341 tgcgccctct tcgccctcat gctgcaacat caccaagccc gtatcggcgc ccactgcgta
     2401 ggtttcatat aaaatctgca acacatgccc agctttggct gaacctattt gccgatatga
     2461 aatacctgct ccagcgctaa tttcagtgag ttcttcagcg cggtaaaaca cttcactttt
     2521 aacctgtggc aattcatctg aaaaaaactc cgccaaagtc attggcactc cagcgagaat
     2581 gcgtttaagc gcactgaccg atggactggt ctgacctgac tcaatcagtg aaatcgttgc
     2641 attggccact cctgcttttt tggcaagggc gcgctgcgag agctgatgcc gtagccgtac
     2701 catgcgtaat cttgccccta aatcttgctc aatcactttt cacctcaatt cttgtgttaa
     2761 tagtgtttaa tatacaataa cagacaaata aataaaacac attaaaacaa tcacttaatt
     2821 ttttaattaa ccatacttga catcgtaagc taaacattta actatgcaca tgttaaagcc
     2881 atttacaaag taaaattcag gaatttcaat gtcaaaagcc agcacaaatc gtttgccaga
     2941 tttatcgcac tactgggtgc catttaccgc caatcggcaa tttaaaacga tgccgagact
     3001 atttaaggct gcgcgcggca attattacac caccatggat gaccggcagg tgctagatgg
     3061 cacagcaggg ctttggtgcg tgcctgcggg gcatggtcgc gaagaaattg ccagtgctgt
     3121 tggcaaacaa ttactcacgc ttgattacgc cccagctttt caatccagtc atccgttagc
     3181 gtttgaggcc gccagccgcg tggcagacta tatgccgagc ggcttagacc gtatattctt
     3241 tactaactcc ggctcagaat cagccgatac cgcattaaaa attgccttgg cgtatcatcg
     3301 cgcctgcggc aacccgttaa gaaacaagct gattggtcgt gaacgtggct atcatggggt
     3361 taattttggc ggcattgcag tgggtggtat tgcgggtaac cgaaaagcat ttggcaacgc
     3421 attaagcggt gttgaccata ttcgccaccc gctcgacatt gccaaaaatg cgtttacgaa
     3481 aggcgtgccg caagacggcg gcattgcgct cgccaatgaa tttgag
//
LOCUS       DAOQSI010000188         3510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_29815_length_3510_cov_4.362952, whole genome shotgun sequence.
ACCESSION   DAOQSI010000188 DAOQSI010000000
VERSION     DAOQSI010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29815_length_3510_cov_4.362952"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..1202
                     /locus_tag="PKL42_09580"
     CDS             192..1202
                     /locus_tag="PKL42_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNU67364.1"
                     /translation="MRRFALVVVLVSNVAPALALEQEKILKALQSVVMIRGYNAGGGL
                     SYGSGVVVAKNTVITNCHIFRSTKEPWVARGEDSYPIESVKADRWRDLCLVSTSTLPL
                     NPAPLGKWDNIKRGQEILSMGHSNGVPNPLTSAGAVKATYMFDGGHVIRSSAKFLMGA
                     SGSGIFDLEGNLLGINTFKTPGRPAYFYSLPIEWLADLEKQPVETTFPIVGKTFWEED
                     DNKKPFFMQVALPEIHQDWPKLAEVAQNWVVAEPKNSDAWYALGEAQENLFKIDEAKQ
                     SYQKSVALDASNTDALFRLGVIAQSAGDKSTEHDIHVAISNINKELAQEYSQLLGCKE
                     EC"
     gene            1301..1795
                     /locus_tag="PKL42_09585"
     CDS             1301..1795
                     /locus_tag="PKL42_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67365.1"
                     /translation="MKFLAMLALLLPLACNAANAEYLKIYLMQPKNTILNKMDGVDEM
                     DRYVKEVEVTINQKLAALTTKPTWGFLVMAVREDGKIKAWVDTDDEVAPEVANIMKTV
                     AEETKAFTVKNGAVVFTLGFGIDGAALPENKMPFPNEWKKVAQCDNEDCKDLDTEAIV
                     LKSW"
     gene            2141..3349
                     /locus_tag="PKL42_09590"
     CDS             2141..3349
                     /locus_tag="PKL42_09590"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNU67366.1"
                     /translation="MAQQAAVLSQRVQAIKESPTLAITAKAGKYKAEGRPIIGLAAGE
                     PDFDTPQHIKDAAKAAIDAGFTKYTPVAGIPGLKKAIVNKFKNENGFDYALNEVIVGV
                     GGKQTIFNLCLAVLNKNDEVIIPAPYWVSYADIALVAEATPVIIECGIEQGFKLQPAQ
                     LEAAITPKTKIFMINSPSNPTGAVYSLDELKALGEVLLKHPHVLVATDDMYEHVNLTG
                     DKFYNILNAAPALKDRCIVLNGVSKAYSMTGWRIGYAAGPAYIIKAMEILQSQSTSNA
                     TSISQVAAQAALEGSQDCIKPMVAAFKERHKYVVDRFNSMPGLSCLMAGGAFYAFADA
                     RKAITQLHLQGKINAATDMALAEYLLESFDVAVVPGSAFGAEGYFRISFATSMDNLKN
                     ALDRIEKALS"
BASE COUNT         1075 a          778 c          734 g          923 t
ORIGIN      
        1 tttaatttta acagcaaatc aggtactggc gatgccctgt gataaatact gaattatcaa
       61 cgactgttga actaacattc acgtccaaag tcatttaaca aacgtttacg ccttacggcg
      121 aatttatgca aaaagttgca ttttatacgt ttaatataaa aaataaataa ttcaatactt
      181 tggagatctc tatgcgtaga ttcgccctgg tcgtcgtatt agtcagcaat gttgcgcccg
      241 cgctggccct tgaacaagaa aaaattctta aagccttaca gtctgtggtc atgatacgtg
      301 gctataacgc tggcggcggc ttatcgtacg gttcaggtgt tgtggttgca aaaaacactg
      361 tcattaccaa ttgccatatt tttcgtagta ccaaagagcc gtgggtagct cgtggtgaag
      421 attcttaccc gattgaatca gtaaaggctg accgctggcg agatttatgt ttagtaagca
      481 cctccaccct gccgctcaac ccagcaccgc ttggtaagtg ggacaacatt aaacgtggtc
      541 aagagatact ttccatgggg cattctaacg gtgtgcctaa ccctttaact tcagcgggtg
      601 cggtaaaagc gacctatatg tttgacggcg gccatgtcat tagaagcagt gcaaaatttc
      661 tgatgggggc aagcggcagc ggtatttttg accttgaagg taatttactt ggcattaata
      721 cctttaaaac accaggcagg ccagcgtatt tttattcact accgattgaa tggctggcag
      781 atttagaaaa acaacctgtt gagacaacat tccccatcgt cggtaaaaca ttttgggaag
      841 aagatgacaa taaaaagcct ttcttcatgc aagtggcgct accagaaata caccaagatt
      901 ggcctaaact ggctgaagta gcgcaaaact gggtagtcgc tgaacctaaa aactccgatg
      961 cttggtacgc gctgggcgaa gctcaagaaa atctattcaa aattgacgaa gcaaaacagt
     1021 cttaccaaaa atctgttgcg ttggacgcca gcaatacgga cgcgcttttt cggttagggg
     1081 ttattgcgca atcggcaggt gacaaatcaa ctgagcatga tattcatgtc gcaatcagca
     1141 atatcaataa agagctcgcg caagagtaca gccaattact tggctgtaaa gaagaatgtt
     1201 aagtccgtaa aaagttgtac cttagcagtg taatacacag caatattgat tttttaaatg
     1261 cctcaattta acgctttacc cacatataaa ggaccctaaa atgaaatttc tcgcaatgct
     1321 cgccctactt ttaccacttg catgtaacgc cgccaatgcg gaatatttga aaatttattt
     1381 aatgcagccc aaaaacacca tcctcaacaa aatggatggc gtagatgaaa tggaccgcta
     1441 tgtaaaagaa gttgaagtga cgattaacca aaaacttgca gcgctcacca ctaaaccaac
     1501 ttgggggttt ttggtcatgg ccgtgcgtga agatggcaaa atcaaagctt gggtcgatac
     1561 cgatgatgaa gttgcgcctg aagtcgccaa cattatgaaa accgtggctg aagaaaccaa
     1621 agcctttacg gtaaaaaatg gtgccgttgt ctttaccctt ggttttggca ttgatggtgc
     1681 ggcactgcct gaaaataaaa tgccgtttcc gaatgagtgg aaaaaagtag cccaatgcga
     1741 taacgaagac tgcaaagatt tagatactga ggctattgtg ctgaaatctt ggtaaaaatt
     1801 gcctgatttt ttactgcgtt taacgtcatt aaaataggta ttttttaatt gttttaagcg
     1861 tatttttaag tccaaccttt ttcgatgctc tggaagcctt ataaacaaag gcttccagag
     1921 catgtttttt tgctcggcaa aataaagtaa tttaaaacgc gcttttcaga catccatttt
     1981 catccctacc tacaacaaag ttgaatagaa attaatagcc ataaatattg caaagattgg
     2041 cagcacagaa cattcctaaa ccactgattt atgcgtaaaa tagcgctcat tcacatctca
     2101 cataaaaatt tgtttaagga aacttatttt gtcatcttcg atagcgcagc aagcagccgt
     2161 tttatctcaa cgcgtacaag ccattaaaga atctccaaca ctcgccatta ctgccaaggc
     2221 cggcaaatac aaagctgaag gccgccctat tattgggtta gctgctggcg agccagattt
     2281 tgatacacca cagcacatta aagatgccgc aaaagccgcc attgatgcag ggtttaccaa
     2341 atacacgccg gttgctggca ttcccggcct taaaaaagcc attgtcaata aattcaaaaa
     2401 tgaaaatggt tttgactacg cactcaacga agtcattgtt ggcgttggcg gtaagcaaac
     2461 aattttcaat ttatgtttag ccgtgctcaa caaaaatgat gaagtgatta tccccgcgcc
     2521 gtattgggtg tcatacgcag atattgcact ggtagcagaa gcaaccccag tgattattga
     2581 atgtggtatt gaacaaggct ttaaactgca acccgcacaa cttgaagccg ccatcacccc
     2641 aaaaaccaaa atattcatga ttaactcacc ttcaaaccca acgggtgccg tgtatagcct
     2701 tgatgaactc aaggccttgg gagaggtgtt actcaaacat ccgcacgtgc tggttgccac
     2761 ggacgacatg tacgagcatg tgaacttaac gggcgacaag ttttacaaca ttcttaacgc
     2821 cgcacctgca ctaaaagacc gttgcatcgt tctaaatggc gtttctaaag cttattcaat
     2881 gacaggctgg cgtattggct atgccgcagg cccagcttac atcatcaaag ccatggaaat
     2941 tctacaaagc caatctacaa gtaacgcgac ttccatttca caagtggccg cccaagccgc
     3001 acttgaaggc tcgcaagact gtatcaaacc aatggttgcc gcgtttaaag agcgccacaa
     3061 atatgttgta gaccgcttta acagcatgcc aggattaagc tgcctgatgg caggcggcgc
     3121 attttatgcg tttgccgatg cgcgtaaagc catcacccaa ttacatttac aaggcaaaat
     3181 caatgccgca actgacatgg cgttggcaga atacctgtta gaaagctttg acgttgccgt
     3241 cgtgccaggc tcagcatttg gcgcagaggg ttacttcaga atttcttttg ccacctcaat
     3301 ggacaacctt aaaaacgcac tcgaccgcat agaaaaagct ctaagttgaa aatattttaa
     3361 atttaaacac gcttagtcat tgaccaaaag ccgcttaatc attattatca cggcttcaca
     3421 aatttaagcc aaaagcaacg tagtttgtga tgttacaccc accgattcct cgatagctca
     3481 gtcggtagag caacggactg ttaatccgtg
//
LOCUS       DAOQSI010000189         3394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_31344_length_3394_cov_4.767595, whole genome shotgun sequence.
ACCESSION   DAOQSI010000189 DAOQSI010000000
VERSION     DAOQSI010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31344_length_3394_cov_4.767595"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            267..1706
                     /locus_tag="PKL42_09595"
     CDS             267..1706
                     /locus_tag="PKL42_09595"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNU67367.1"
                     /translation="MIKMNKSVRFSTMLAGFIVTTCLCQKIDAAEMYEQLSLNDAIGI
                     ALKNNVEQRISLQAAAVAESQYQEAVSAHWPTVSLQASMTRMDEDPTFSLSGATVSIA
                     SLAAPLNGALFGGAPVIPSTINSGAQKVKLMDRDTATATLSMMMPLYTGGKITSIVNQ
                     AKLGKAIAQEEYHRSTLQVVRDVKRYYYAVQLTQQLTTLAHDTVESLQTTRDLTKSLY
                     EGGSASINKLDYLKTEMAVNYAKSLETEFVAKQKSATAALIHAMGVSWNSEIRIAESA
                     FPKTANQPTLDALVSQAQQFNPQMGMIQLAVKVADERVNEAKSAYYPQVALTGNVRHI
                     ENSYNGGMVNEDNKNSWTIGVVMSMPLFDFGKTSHHVNTAKLQRNQMAETQVLVEQGL
                     AAQIKNLFIGLDAASQQIALSEKTVAFSRDYERLTQQAYQIGASKPEDMVQASIYNAV
                     VEGSYLKAQHDGAYSMAEIEYFIGAQLSN"
     gene            1719..2615
                     /locus_tag="PKL42_09600"
     CDS             1719..2615
                     /locus_tag="PKL42_09600"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HNU67368.1"
                     /translation="MNRSFAKLITIFLLTGSLLAQAASEPFTLIVGSSVIGTRENSKQ
                     DVHLGFNALFNHMISDENIQSEFKDYDKSDLLMQALKNNEVNAMFGSPLEYIQSESWL
                     FDGYLISGLIGKQYKSKMLILIRNDSEIKTIEQLKGKKIAVQRGVIQDLGGLYLETLL
                     LEKNLSLPAQYFSEIVKTDTSNVALVNLFFKKVDVALMSESEFNVAAELNPQMRLQIK
                     AIESSDSYINFVAATTKAVPIDKREAIKKNMLAIEKSAKGRNMLRLLKIDGFKVINLN
                     ELTSVRALVEKNKQLKANLHAK"
     gene            2605..>3394
                     /locus_tag="PKL42_09605"
     CDS             2605..>3394
                     /locus_tag="PKL42_09605"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HNU67369.1"
                     /translation="MRNNFNLFEVLDVSAGKIAFQKLLLFSVTLMLLTISFSSVARPE
                     YVVGLTQQGIRIASVEEVELGFNYELEKISKDKDYALKIKVFSSDVELNNMLQARIIK
                     GYFGSPMMFIENMRDFDASRLFVPVIGNKVKQRYVILVRKDSGVNQLAQLAQKEIAYC
                     TTDEVGALYLQHALKQKGLGTVDGFFSKKIIKKNPSLAATAVFFKETDAAIILESDYL
                     IAAELNPQLLKQMQSIETSPEYLTHLLAFSNEHGFAEKFNIEDNV"
BASE COUNT         1105 a          633 c          749 g          907 t
ORIGIN      
        1 agtttaaaaa aggctaaaaa ataacatttt tagccttttt tatcgtttaa agccgtttat
       61 atattgtatg gctgccagcc acattcaact tggcacgaag tttgccatat ttatcttaaa
      121 gatcatttca agcaaaaaat attagtaatg atttttgttg tatacgcctt tagttcagca
      181 aaaaaagccg ttaattaata gtaacttagg aaattagttt tttacaattt accgaggttt
      241 tagattaacc atcaggaaga ttcagcatga ttaaaatgaa caaaagcgtt cgcttttcaa
      301 cgatgttggc aggatttatt gtgaccacat gcctgtgtca aaaaatcgac gcagccgaaa
      361 tgtatgaaca gctaagttta aatgatgcga ttggtattgc acttaaaaat aatgtggagc
      421 agcgtatttc gctgcaagcg gcagcggttg cagaatcgca atatcaagaa gcagtttcag
      481 cacattggcc aaccgtgagt ttgcaagcca gcatgacgcg catggatgaa gatccgacat
      541 ttagcctttc tggtgctacc gtgtctattg ccagcttggc cgcaccactc aatggggcgc
      601 tatttggggg tgcgcctgtg attccatcaa ccatcaattc tggcgcacaa aaagtcaagc
      661 tgatggaccg tgataccgcc actgccacgc tcagcatgat gatgcctttg tatacgggtg
      721 gaaaaatcac cagcattgtc aaccaagcca aactcggtaa agcaattgca caagaagaat
      781 atcatcgatc taccttgcaa gtggtgcgtg acgtgaagcg ttattactac gcagtgcaac
      841 tcacgcagca attaacaacg cttgcacacg atacggttga atctttgcaa acaacccgcg
      901 atttaaccaa aagcctgtat gaaggaggct cggccagtat taataagctt gattatctca
      961 aaaccgaaat ggcagtcaat tatgcaaaat cactggaaac cgaatttgtt gctaaacaaa
     1021 aatcagccac cgcagcactg atacatgcca tgggggtgtc ttggaacagc gagattcgta
     1081 tagcagaaag cgcatttcca aaaacggcca atcaaccaac cttagatgca ttggtttcac
     1141 aggcgcagca atttaatccg caaatgggca tgattcaatt agccgtaaag gtagcggatg
     1201 agcgggtgaa tgaggccaag agcgcctatt acccgcaagt ggcgcttaca ggcaatgtgc
     1261 gtcatattga aaatagctat aacggtggca tggtgaatga ggataacaaa aactcatgga
     1321 cgataggtgt ggtcatgagc atgcctttat ttgactttgg taaaaccagc caccacgtca
     1381 acacagcaaa actgcaacgt aaccaaatgg ctgaaacaca ggttttggta gagcagggct
     1441 tggctgcaca aattaaaaac ttgttcatcg gcctagacgc tgcaagccag cagatagcac
     1501 tctcagaaaa aacggtggca ttttcgagag attatgagcg tcttacacag caggcctacc
     1561 aaataggcgc aagtaaacct gaagacatgg tacaagccag catttacaac gcggttgtag
     1621 aaggcagcta tttaaaagcg caacatgatg gcgcctatag catggcggaa attgaatatt
     1681 ttattggtgc acaactgagt aattagcaag gcctaaacat gaatcgcagc ttcgcaaaat
     1741 taattactat ttttctgtta acgggtagct tgctagcgca agctgccagc gaacctttta
     1801 cgctgattgt tggtagttcg gtaattggta cgcgtgaaaa cagtaaacag gatgtgcatc
     1861 ttgggtttaa tgcgttattt aaccacatga tttcagacga aaatattcaa agtgaattta
     1921 aagattacga taaatctgac ctcttaatgc aggccctaaa aaataatgaa gtcaatgcca
     1981 tgtttggcag cccgcttgaa tatattcaat ctgaatcatg gttattcgat ggttatttaa
     2041 ttagcggcct aattggtaag cagtacaaat caaaaatgtt gattctaatt agaaatgaca
     2101 gcgaaattaa aacgattgaa cagctaaagg gtaaaaaaat tgcagtgcag cggggagtca
     2161 tacaagattt gggcggtttg tatttagaaa cactgttact ggagaaaaat ctttcgttgc
     2221 ctgcgcaata tttttctgaa attgtaaaaa ccgacacctc taatgtggcg ttggtcaatt
     2281 tgttttttaa aaaagtggac gtcgcgctga tgagtgaaag cgaatttaat gttgcggcag
     2341 aattaaatcc acaaatgcgc ttgcaaatca aagcgattga atcttcggat agttatatca
     2401 attttgttgc ggccaccaca aaagccgtac caattgacaa gcgagaagcc attaaaaaaa
     2461 acatgttggc gatagaaaaa tctgctaaag gtagaaatat gctacggctg ctcaaaattg
     2521 atggatttaa ggtaatcaac ctaaacgaat taacaagtgt acgtgcgttg gttgaaaaaa
     2581 ataagcaatt aaaagcaaat ctacatgcga aataatttca atttatttga ggtgctggac
     2641 gtaagtgccg gcaagatagc ctttcaaaag ctgttgttgt tcagcgtgac gttgatgctg
     2701 ctgacgatta gcttttcaag tgttgcaaga cccgaatatg tggtgggttt aacccagcaa
     2761 ggtattcgta tcgcgtcggt tgaagaagtg gagctggggt ttaattacga actggaaaaa
     2821 atttcaaaag ataaagacta cgcgctaaag attaaagttt tttcgagtga tgttgagtta
     2881 aacaatatgc tgcaagcacg cataattaaa ggctattttg gtagcccgat gatgtttatt
     2941 gaaaacatga gagattttga tgccagccgt ttgtttgttc ctgtaattgg caataaggta
     3001 aagcagcgtt atgtgatttt ggtacgcaaa gatagcggcg tgaatcaatt ggcacaatta
     3061 gcgcaaaaag aaatcgccta ttgcacgacc gatgaagtgg gtgctttata tttgcaacac
     3121 gcattaaagc aaaaagggct tggcacagta gatggatttt ttagcaaaaa aatcatcaaa
     3181 aagaatccaa gtcttgcagc aacagcggtt ttttttaaag aaaccgatgc tgccatcatt
     3241 cttgaaagtg attacttaat cgcagcagaa cttaacccgc aattgctaaa acagatgcaa
     3301 tctattgaaa catcacccga atacttgaca cacttattgg cgtttagcaa tgaacatggc
     3361 tttgccgaaa aatttaatat tgaagataat gttg
//
LOCUS       DAOQSI010000190         3381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_31527_length_3381_cov_4.857186, whole genome shotgun sequence.
ACCESSION   DAOQSI010000190 DAOQSI010000000
VERSION     DAOQSI010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31527_length_3381_cov_4.857186"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1740
                     /locus_tag="PKL42_09610"
     CDS             <1..1740
                     /locus_tag="PKL42_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HNU67370.1"
                     /translation="HAGGIKGFVEYINRTKTVLHPKTFYAKGEKDGMTIEVAMQWNDS
                     YSETVQCFTNNIPQRDGGTHLTGLRTAMTRTLNQYIEQTELAKKAKVETTGDDMREGI
                     TCVLSVKVPDPKFSSQTKDKLVSSEVQPVVSEVVSAKLAEFLQENPADAKIICGKIVE
                     AARAREAARKAREITRRKGVMDTLGLPGKLADCQERDPSKCEIYLVEGDSAGGSAKQG
                     RDRKNQAILPLKGKILNVEKARFDKLLGSQEIATLITALGTSIGKADFNVEKLRYHRI
                     IIMTDADVDGAHIRTLLLTFFYRQMTELVERGHIYIAQPPLYKVKQGRDERYLKDQQE
                     LDVYLLNSALKGAELDTKTDAGILADAPLAEIAHQVVLTEAVIRRISSLYDESVLRAI
                     QDAGEINLSNELNANRIAEKLRPILGAIGSEVIVAFDAESNTYRLEVNKFVHGNLQSC
                     VIDAEFLSSGDYRQILKTSTMLQGLVGAGAVIKRNDKEQAVSTFKQALDWLLAEAKNT
                     LNIQRYKGLGEMNPEQLWETTMDPAVRRLLKVQIDDAIAADEIFTTLMGDNVEPRRAF
                     IESNALGASNLDI"
     gene            complement(1974..2942)
                     /locus_tag="PKL42_09615"
     CDS             complement(1974..2942)
                     /locus_tag="PKL42_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNU67371.1"
                     /translation="MNELQSISTWWMWAGFTIFVIIMLGVDMFALGRKGAHKVGAKEA
                     LVWSIIWFALAMLFGGLLWGWLDHTAGRAVADTKVMEYLTGYLLEKTLAMDNIFVFVM
                     IFSYFAVPLEFQKRILIYGVLGAIILRALMILLGAWLIAQFHWVLYLFGAFLLITGIK
                     MFIFAEHEPDLAKNPLLNWLRNHFRITENYHGDKFWVMQNGVRWFTPMFLVLVLIEFS
                     DVIFALDSIPAIFAVTNDPFIVFTSNIFAILGLRALYFLLADMADRFHLLKYGLAVVL
                     IFVGTKMLIVEWYKIPVAISLAVVISVLGISMLLSILSTRKTKQIS"
     gene            complement(2960..>3381)
                     /locus_tag="PKL42_09620"
     CDS             complement(2960..>3381)
                     /locus_tag="PKL42_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNU67372.1"
                     /translation="FLTAFVQGVVAGLGYWVAGLSSPVMFGLLTMLLALIPFCTPIAW
                     GTAGLLLFMQGETGAAIGVWLWGAIVVSQLDNFLRPIFISSVSPIPFLFVLFGVLGGL
                     LAFGLVGLFIGPIILAVIWAVWREWVTHLQDEVLSEN"
BASE COUNT         1090 a          780 c          766 g          745 t
ORIGIN      
        1 cacgcaggcg gcattaaagg ctttgtagag tacattaacc gtacaaaaac agtgctgcac
       61 ccaaaaacct tttatgccaa aggcgaaaaa gacggcatga cgattgaagt tgccatgcaa
      121 tggaatgaca gctattccga aacggtgcaa tgttttacca ataatattcc acagcgcgat
      181 ggcggcacgc acttaaccgg gctacgcacg gcaatgacgc gtacgcttaa tcagtatatt
      241 gaacaaaccg aactggcaaa aaaagcaaaa gtagaaacca caggcgatga catgcgagaa
      301 ggcataacct gtgtgttatc cgttaaagtg ccagacccaa aattttcatc ccaaaccaaa
      361 gataaactgg tgtcgtcaga agtgcaaccg gtcgtttcag aagtggtttc cgcgaaactg
      421 gcagaatttt tacaagaaaa cccagcagat gccaaaatta tctgcggcaa aattgtagaa
      481 gccgcgcgcg cgcgcgaagc agcacgtaaa gcgcgagaaa tcacccgccg taagggcgtg
      541 atggatacgc ttggcttacc tggcaaactg gccgactgcc aagagcgcga cccaagcaaa
      601 tgtgaaatct accttgtcga gggtgactca gcgggcggct cagccaaaca agggcgcgac
      661 cgtaaaaacc aagccatttt accgctcaaa ggtaaaattc tgaacgtcga aaaagcacgt
      721 tttgacaaac tattgggctc gcaggaaatt gcaaccctta ttacagcact tggcaccagc
      781 atcggtaaag ccgattttaa tgttgaaaaa ttacgttatc accgcattat catcatgacc
      841 gatgcagacg tggacggtgc gcatattcgt accttgttgc tcacattctt ctaccgtcaa
      901 atgaccgagt tagtagaacg cggccatatt tacattgcac agccaccgct ttacaaagta
      961 aaacaagggc gggatgagcg ctatttaaaa gaccagcaag aattggatgt ttacttgctc
     1021 aactcagcgc tcaaaggtgc agaacttgat acaaaaaccg atgcgggtat tttagcggat
     1081 gcgccgttag cagaaattgc gcatcaagtg gtattaactg aagcggtgat tcgccgtatt
     1141 tcatcgttgt atgatgaaag tgtgttgcgc gccattcaag acgcaggtga aattaacttg
     1201 agtaatgagc tcaatgccaa tcgcatcgca gaaaaattgc gcccgatact tggcgcaata
     1261 ggcagtgaag tgattgtcgc ttttgatgct gaatcaaaca cttaccgttt agaagtgaat
     1321 aaattcgtcc acggcaattt gcaaagctgc gtgattgatg cagagttttt aagtagcggt
     1381 gattaccgcc aaatacttaa aacctccacc atgctgcaag gtttagtagg tgctggcgcg
     1441 gtgattaaac gtaacgacaa agagcaagcg gtaagtacgt ttaaacaggc gctcgactgg
     1501 ctgttggcag aggctaaaaa cacgctcaat attcaacgct ataaaggctt gggtgaaatg
     1561 aacccagagc agttatggga aaccaccatg gacccagcgg tgcgccgttt gctcaaagtg
     1621 caaattgatg atgccattgc cgccgatgag atattcacca ccctgatggg cgataatgtg
     1681 gaaccaaggc gtgcgtttat tgaaagcaat gcattgggtg caagtaacct cgatatttaa
     1741 ctttgaactc ctttgattgt ggattaattt cttgaaataa atcctaagta aaaagacaaa
     1801 aagacgtgct aacagcacgt cttttttatg ggcattaaaa gctaaaaatt taactaaaaa
     1861 tcgcctaaaa ttaacacaac ctttttcgat gctctggaag ccttataaat aaaggcttcc
     1921 agagcattat ttttttctcg cgcgaaaagg ccggccgttt tattaaaaaa tcgctatgaa
     1981 atttgtttgg tttttcgggt agatagaatg cttaatagca tgctgatacc aagcaccgaa
     2041 atgaccacag caagtgaaat ggccacgggt attttgtacc attcaacaat cagcattttg
     2101 gtgccaacaa aaatgagtac aacggctagg ccgtatttaa gcaggtgaaa gcggtctgcc
     2161 atatctgcga gtaaaaaata aagcgcacgc aagcctagaa tggcgaaaat atttgaggta
     2221 aagacgatga aggggtcgtt cgtcactgca aaaatggcgg ggatgctatc gagcgcaaaa
     2281 atgacatcgg aaaattcaat gagcaccaag actaaaaaca tgggggtgaa ccagcgtacg
     2341 ccgttttgca tgacccaaaa tttgtcaccg tgatagtttt ctgtaatacg gaagtggttg
     2401 cgtaaccaat ttaatagcgg atttttggca aggtctggct catgctccgc aaaaataaac
     2461 attttaatgc cggtgatgag taaaaacgcg ccaaatagat acagcaccca atgaaactgc
     2521 gcaataagcc aggcgccgag taaaatcatg agggcgcgca aaataatagc ccccagcaca
     2581 ccataaatga ggatgcgttt ttgaaactca agcggcacgg caaaatagct gaaaatcatg
     2641 acaaaaacaa aaatattgtc catggccagc gttttttcaa gcaggtaacc cgtgagatat
     2701 tccataactt tggtatctgc caccgcacgc ccagccgtgt ggtcgagcca gccccataat
     2761 aggccaccaa acagcatcgc taaggcaaac caaataattg accacaccag cgcttctttg
     2821 gcaccgactt tatgtgcgcc tttgcggcca agcgcgaaca tatcaacgcc taacatgata
     2881 atcacgaaaa tagtaaaacc ggcccacatc caccacgtgc taattgattg taattcgttc
     2941 attcaacatc ctttagagat taattttcgg ataacacttc atcttgtaaa tgcgtgaccc
     3001 attcacgcca aaccgcccaa atcacagcta agataatggg cccaataaat aagccgacca
     3061 agccaaaagc gagcaggccg cctaatacgc caaacaacac aaacaaaaat ggtatggggc
     3121 tgacgctgct aataaagatg gggcgcaaaa agttatctaa ctggctcacc acgatggcac
     3181 cccaaagcca aacgcctatg gccgcccccg tttctccttg cataaaaagc aataaaccag
     3241 ccgtgcccca ggcaattgga gtacaaaagg gaatgagggc gagcaacatg gtgagtaagc
     3301 caaacatcac aggagaggac aaccccgcca cccaataacc caagcccgcc acaacgccct
     3361 gcacaaaggc agttagaaag a
//
LOCUS       DAOQSI010000191         3379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_31573_length_3379_cov_3.475030, whole genome shotgun sequence.
ACCESSION   DAOQSI010000191 DAOQSI010000000
VERSION     DAOQSI010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31573_length_3379_cov_3.475030"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1822)
                     /locus_tag="PKL42_09625"
     CDS             complement(<1..1822)
                     /locus_tag="PKL42_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67373.1"
                     /translation="MKNTFSLRRASLQLIAGFVLIGAFNAGSSFYFSKQVLAAQDELK
                     LVVEANNLAKHTLFNVSQVQQFLTDVGATGERTGFAEAQENYDAAKQQLSTIAETHSE
                     LSVQAKKVSDSLASFNQVGIAMAEAYLAKGREAGNIAMKTPQTGFDARAETLAEDLNE
                     LTQALDKQNNELTLAVEGSLAQLRTSSLIVNVISIALLVALLMTVGRRLKQYLGGEPS
                     EAHAIALQIAEGRFDQIKANKNADNANVIGAINNAAAKIAEMEHGMMRMESEHVKGNI
                     DVRIDVSTLPNDYRRMADGINRIAENHIDVLKKSTACITALAKGDFEVQLEKFPGQLA
                     LVNEGVEGLRYNIKTLISDMRHMSDEHQKGNISFMIDPAKFSGDYQLMADGVNDMVNE
                     YIDENKTVMACVEQFGNGDFSATIKEYPGEKAFINKGIKKIGGNLKGLIDSVNYVSGE
                     HEKGAIDMTLRDDMFKGDFSILAKCVNKMMAGILEMNEKAMAVVKAFGEGRFDEPLEQ
                     FPGKKAEINTMIEQVRSNLKALNDDAQMLATAAHDGRVSVRADANAHQGDYRKIIESM
                     NETLEMIVGPIGTVKVAVETINTAAKEISQGNADLSRRTEE"
     gene            complement(1925..3139)
                     /locus_tag="PKL42_09630"
                     /pseudo
     CDS             complement(1925..3139)
                     /locus_tag="PKL42_09630"
                     /inference="COORDINATES: protein motif:HMM:NF016830.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
BASE COUNT          901 a          818 c          651 g         1009 t
ORIGIN      
        1 gttcttcggt gcgacggctt aggtctgcgt tgccttgcga gatttctttg gcagctgtgt
       61 tgatggtttc taccgcgact ttgacggtgc cgatggggcc gacgatcatt tcgagggttt
      121 cgttcatgct ttcaatgatt ttacggtaat ccccttggtg tgcatttgca tcggcacgta
      181 ctgatacacg gccatcatgt gcggctgtcg ctagcatttg cgcatcatcg ttgagtgctt
      241 ttaggttgct acgcacttgt tcaatcattg tattaatttc ggcttttttg cctggaaatt
      301 gctctagcgg ttcatcaaaa cggccttcac cgaacgcttt tactacggcc atggcttttt
      361 cattcatctc taatatgcca gccatcattt tattcacaca cttagccagt atgctaaagt
      421 cacccttgaa catatcatca cgcaaggtca tgtcaattgc gcctttttca tgctcgccac
      481 tcacataatt caccgaatca atcaaacctt tcaggttacc gccaattttt ttaatgcctt
      541 tgttaataaa ggctttttca cctgggtact ccttaatggt ggctgaaaaa tcgccgttac
      601 caaactgctc tacacaggcc atcacggttt tattttcatc aatatactca ttcaccatgt
      661 cattgacacc atcggccatt agttggtaat cccctgagaa cttggcgggg tctatcataa
      721 aactgatatt gcctttttgg tgttcatcag acatgtggcg catatcacta atcaacgttt
      781 taatgttata gcgcaaacct tcaacaccct cattcaccag tgcaagctga ccaggaaact
      841 tctctaactg cacttcaaaa tcacctttgg ccagtgctgt tatacaggcg gtagattttt
      901 taagtacatc aatatggttt tcagcaatgc ggttaatgcc atctgccatg cggcgataat
      961 cgtttggtaa cgtgctcacg tcaattctga catctatatt acctttgaca tgttcagatt
     1021 ccatacgcat catgccatgt tccatctcag caatttttgc ggctgcattg ttaatcgcac
     1081 ctatcacgtt ggcattgtct gcatttttgt tggctttgat ttggtcaaac cgaccttcgg
     1141 caatttgtag cgcaatagca tgcgcttcac ttggttcacc gcctaaatac tgtttaagcc
     1201 tgcggcccac tgtcattaac aatgcaacaa gcagcgcgat tgaaatgaca tttacaatca
     1261 aactactggt acgtaattgc gctaagctac cttctaccgc caaagttaac tcgttgtttt
     1321 gcttatcaag cgcttgcgtc agttcgttta aatcctctgc caatgtttcg gcacgtgcat
     1381 caaagccagt ttgcggcgtt ttcatggcga tattgcctgc ttcgcggcct tttgcaaggt
     1441 aagcttcagc catggcaatg cctacttgat taaagctcgc taatgagtcg cttacctttt
     1501 ttgcttgcac actgagttca gaatgagttt cagcaattgt gcttaattgc tgtttggcag
     1561 catcatagtt ttcttgcgct tctgcaaaac cagtacgctc accggtggcg ccaacatcgg
     1621 ttaaaaattg ctgcacttgt gatacattaa acaacgtgtg cttggctaaa ttattcgcct
     1681 caactacaag tttaagttca tcttgtgccg caagcacctg ctttgaaaag taaaaactgc
     1741 tgcccgcatt aaatgcgcca atgagtacaa acccagctat cagttgcaaa cttgcacgac
     1801 gtaaactaaa cgtatttttc atcagtattt ccaaatctat ctatagttat ctatcatgat
     1861 tatcgcgcta atacattgtc attaaagtca taaatagcgc tataaaattt acttactttt
     1921 aaatctaaaa agtaatttcc gttcttaaac caagcacagt gacattttta atgcccttat
     1981 ccatactggg gttggtgatg tattgcaagc ttggctgaat agcgaaatat ggctttaact
     2041 gcacacggta cgtcgcttca taattcactt cttggtgttc ggcattattt gcttgacgat
     2101 aatttttacc attatggttg actgtgactg caacgcccgc aacatcatca tcgcgccctg
     2161 aaattaagcc gtgataacgt aagcccaaac tgccagacca atctatttga ttcacttttt
     2221 cggtagaacg accaaaacgt acaaacccag ctaacccttg tgatggctgc gctgacccag
     2281 caaataaact ccgttcagca attacataaa ccccatggct tcgatggcga ctatccgacc
     2341 catcaattgc atcaaactga ttgctgtagc cccaaaaacc aatagcggtt ttgttgaagc
     2401 ttcccacttg ctcggttgaa attgcctcgg cgcgcggcgt ataacctatt tcaacaattg
     2461 aaaacgtgcc atcaccattg ccaagtttaa tatgcgtgcc gcgtgggtca tctgggtcac
     2521 caggcacgcc atcggtaatg gctgcttgca tataaaaatg ctcatttggc gaagtcaatt
     2581 ttaaccgcac cgccagtgcg cctaatggaa aaaccggcgg cccattcaca ccggtttggg
     2641 cgatttagtt ggccatgcca taaggtggct gtataaaaat gcctgatgtt tcatgcacat
     2701 aaaactcagt atcaactggg tacaaccccg ccaacactga aacatgacca tctaaaaagt
     2761 ttttctgtag ccatgcttca taaaattgtg cggtattggt acccacttca atgttatcga
     2821 cgcccataaa ggcacccaca taatgcgtat taaatttact gcctaaatcg ctctgataaa
     2881 caaaattggc cgtagcttca ctaaaaccta aaagtttatc caaatcaaaa gtggccctaa
     2941 tttcagtatg gcccagccaa gccgtgccgc gctttaaacc accagaaaca ttcgatagca
     3001 catcactttt gtgcaacacg ccaatatcaa cgcccttggc gtaccacgcc tgacgcaatc
     3061 caccccaatc gccggtaagt gtatcttcct gccaattggg agatggcgcc tcttctgcct
     3121 gtgcctgatg actctgcatc gcaaacacga gcaggcttgc ggtaataaaa gaaactaaaa
     3181 gtggcttttt aggctgaact aaactttgat gtaaaccggg catacaattt ttaccaatca
     3241 attaactaaa actaaacggt tgaccgcgaa gtgcttagta attacaacgg taaaaaatgc
     3301 tagaacttga tattgtgggg aagattcttt tgtaaattta agtaaatttg gaacagaaaa
     3361 tgaaaaggat ttaactagt
//
LOCUS       DAOQSI010000192         3355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_31911_length_3355_cov_4.913636, whole genome shotgun sequence.
ACCESSION   DAOQSI010000192 DAOQSI010000000
VERSION     DAOQSI010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31911_length_3355_cov_4.913636"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..130)
                     /locus_tag="PKL42_09635"
     CDS             complement(<1..130)
                     /locus_tag="PKL42_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HNU67374.1"
                     /translation="MNNLTLQAEVVQIEPLRYTPAGLPLLSVVLRHVSEQVEAGMKR"
     gene            complement(139..534)
                     /gene="rpsF"
                     /locus_tag="PKL42_09640"
     CDS             complement(139..534)
                     /gene="rpsF"
                     /locus_tag="PKL42_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNU67375.1"
                     /translation="MRHYELVFIVHPDQSEQVPAMIERYRTLITSNGGAVHRLEDWGR
                     RQLAYPIQKLHKAHYVLMNIEVNLEVLAELEHGFKFNDAVLRHLLIAKKTAVTAPSPM
                     MKEEKSKSVLSRDAAPVAPAADATEVAAA"
     gene            complement(724..1218)
                     /locus_tag="PKL42_09645"
     CDS             complement(724..1218)
                     /locus_tag="PKL42_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HNU67376.1"
                     /translation="MQTVIENVTAASHVIQQAVAPVFLLTGIGAILSVLTGRLGRVVD
                     RFRVLNEKYDAAKQVLAEQPEYAKAIHELYILQCRAEWVHWAITLSTLSLLFVALVIG
                     ILFLGSAISWDLSWLVSPLFILAMLTLVLSLGCFLREITLSKHTFELPSNKNRVLKPF
                     SKKD"
     gene            complement(1305..3338)
                     /gene="uvrB"
                     /locus_tag="PKL42_09650"
     CDS             complement(1305..3338)
                     /gene="uvrB"
                     /locus_tag="PKL42_09650"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNU67377.1"
                     /translation="MFVTFPNSKYQLYQPFPPAGDQPQAIDKLTQGIESGLKFQTLLG
                     VTGSGKTFTMANVIARTGRPAIVMAPNKTLAAQLYSEFREFFPNNSVEYFVSYYDYYQ
                     PEAYVPSRDLFIEKDSSINEHIEQMRLSATKALLEREDSIIVATVSAIYGIGDPVDYH
                     GMVLHVQQGEKMAQRDMITRLIGMQYDRNDFDFSRGTFRVRGDVIDVFPAENNETAVR
                     ISMFDDEIDSITLFDPLTGQIFNKIPRYTVYPSSHYVTPRETTIKAMEKIKQELRSRV
                     DFYIKENKLVEAQRIEQRTRFDLEMLNEIGFCKGIENYSRHLSGRNPGDPPPTLIDYL
                     PNNALMIIDESHVTVPQIGGMFLGDRSRKTNLVDYGWRLPSAMDNRPLKFEEFERIMR
                     QCVFISATPAEYEKNHQQQVVEMIARPTGLIDPEIVVKPADTQVDDLLGEIKKRVEVG
                     ERVLATTLTKRMAEDLTDYLSEHGVKTRYLHSDIDTVERVEIIRDLRLGEFDVLVGIN
                     LLREGLDIPEVSLVAVLDADKEGFLRSERSLIQTAGRAARNLNGQVIFYANKITRSMK
                     LAMDETERRRGVQMAFNTLHGIVPKGISKRIKDIIDGVYDKDEAERERKSLKEQANFE
                     SLSEKQLVKEMKRIEKAMHDAAKNLEFEKAADYRDQLKKLKLKVFGAEMPDVI"
BASE COUNT          937 a          796 c          666 g          956 t
ORIGIN      
        1 tacgcttcat gcctgcctca acttgctcag aaacatgacg caatacaaca cttaacaacg
       61 gtaatcctgc tggcgtgtag cgaagcggct caatttgcac gacttctgct tgcaacgtca
      121 gattattcac ttattacttt aggctgctgc cacctctgta gcatccgctg caggggcaac
      181 tggtgcggca tcacggctca acaccgattt tgatttttct tctttcatca tcggtgaagg
      241 tgcagtcact gcagttttct tggcaatgag caaatgacgc aatacagcat cattaaactt
      301 aaagccatgc tcaagttctg ctaatacttc aaggttcact tcaatgttca ttaacacgta
      361 atgggctttg tgaagttttt gaattgggta agccaattga cgacgacccc agtcttctaa
      421 acgatgcact gcaccgccgt tgctggtgat tagcgtacga tagcgctcaa tcatcgctgg
      481 cacttgttcg ctttggtccg gatggacgat aaatacgagt tcgtaatgtc tcattaatac
      541 tccttttggg ttaaagccac ctgcaaagct ctttaattta gagcgacaac ttaaatatgg
      601 ttgtattgca gtgtagcaag ggggtttgat tacttgccaa ataacaacat tcggcaagcg
      661 cgcgattata atcaaaagca cacactaaat caacctaaaa cggaatttaa ctaccgaagg
      721 cttttaatct tttttagaaa acggctttaa cacgcgattt ttattgcttg gtaattcaaa
      781 tgtatgtttt gacagtgtaa tttctcttaa aaaacaccct aaactcagca ccagtgtcag
      841 cattgctaaa ataaataagg gtgaaaccag ccaagataaa tcccaactaa tggcagagcc
      901 caaaaacaaa atgccaatga ctaaagccac aaataaaagc gacaaggtac ttaaagtgat
      961 ggcccaatga acccattctg cacgacattg caaaatatag agctcatgaa tggccttggc
     1021 atattctggt tgttcagcca gtacttgttt tgcggcatca tatttttcat ttaacactct
     1081 aaagcgatca accactcgcc ccaagcggcc tgttaacaca cttaaaatgg ccccaatgcc
     1141 tgttaacaaa aacacagggg caacggcctg ctgaatcaca tgagaggccg cagtgacgtt
     1201 ttcaatcaca gtttgcataa tttaacctta tacattaact tatatattta atttattgaa
     1261 tattaataat attttacaaa gatatgaata tcatttatag cgcatcaaat aacgtccggc
     1321 atctctgccc caaaaacttt taactttaat tttttaagtt gatcgcgata gtcggccgct
     1381 ttttcaaact ctaaattttt agccgcatca tgcatggctt tttcaatgcg cttcatttct
     1441 ttcacgagtt gtttttcact caaactttca aagttagcct gctctttaag cgatttacgc
     1501 tcgcgctcgg cttcatcttt atcataaaca ccgtcgataa tatctttaat acgcttacta
     1561 atacctttgg ggacgatgcc gtgcaacgtg ttaaatgcca tttgcacacc acgtctgcgc
     1621 tcagtttcat ccatcgcgag cttcatgcta cgggtaattt tattggcata aaaaatcact
     1681 tggccattta agttacgcgc tgcgcgccct gctgtttgaa tcaaactacg ctcagatcgc
     1741 aaaaagcctt ctttatcggc atctaacaca gcaaccagcg acacttctgg aatatctaag
     1801 ccttctcgca ataaattaat gcccaccagc acgtcaaact cacctaggcg taaatcgcga
     1861 ataatctcga cacgctcaac tgtatcaata tcgctgtgta aataacgcgt ttttacgcca
     1921 tgttcactta aataatcggt taaatcttcc gccatacgtt tagtgagcgt tgtggctaac
     1981 acacgttcgc ccacttccac ccgtttttta atttctccta acaaatcatc cacttgcgta
     2041 tcagcaggct tcaccacaat ttcagggtca attaaacctg tggggcgcgc aatcatctca
     2101 accacttgtt gctggtgatt tttttcatac tccgcgggtg tagccgaaat aaacacacac
     2161 tggcgcataa tacgttcaaa ttcctcaaat ttcagtggcc ggttatccat ggctgacggt
     2221 aaacgccagc cataatccac caaattcgtt ttacgcgaac ggtcgcccaa aaacatgccg
     2281 ccaatttgtg gcactgtcac gtggctttca tcaataatca tcagggcatt attgggcaga
     2341 tagtcaatca gcgtaggcgg cgggtcgccc ggattgcggc cagataaatg ccgcgagtaa
     2401 ttttcaatgc ctttgcaaaa gcctatttca ttgagcattt ccaaatcaaa tcgggtgcgc
     2461 tgttcaatac gctgcgcttc aactaattta ttttctttga tgtaaaagtc gacacggcta
     2521 cgtaattctt gtttgatttt ttccatcgct ttaatggtcg tctcgcgcgg cgtcacgtaa
     2581 tgactggatg gatacacggt gtaacgtgga attttattaa aaatttgccc ggtgagcggg
     2641 tcaaacaaag taattgaatc aatttcatca tcaaacattg aaatgcgcac agcggtttca
     2701 ttattttcgg ctggaaacac gtcaatcaca tcgccgcgca ctctaaaagt tccgcgcgaa
     2761 aaatcaaagt cgttgcggtc gtactgcatc ccaatcaggc gcgtaatcat atcgcgctgg
     2821 gccatttttt cgccttgttg cacgtgcaac accatgccat gataatccac ggggtcgcca
     2881 atgccataaa tggccgaaac agtggcaacg ataatgctgt cttcgcgctc taaaagtgct
     2941 tttgtggccg aaaggcgcat ctgttcgatg tgctcattga tactggagtc tttttcaatg
     3001 aataaatcgc gtgaaggcac gtaggcttca ggctgataat aatcgtaata gctgacaaaa
     3061 tattccaccg aattgttggg gaaaaactcg cggaattcac tatatagctg tgctgcaagt
     3121 gttttgttgg gcgccatgac aatggccggg cgaccggtac gcgcaatcac attggccatg
     3181 gtgaacgttt tgccagaccc cgtaacgccc agcagggttt gaaacttcag cccactttca
     3241 atgccttgtg taagcttatc aatcgcttgc ggctggtcac cagcaggcgg aaaaggctgg
     3301 taaagctgat atttactgtt aggaaaagta acgaacacag gatgctttgc ttaaa
//
LOCUS       DAOQSI010000193         3320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_32417_length_3320_cov_3.866462, whole genome shotgun sequence.
ACCESSION   DAOQSI010000193 DAOQSI010000000
VERSION     DAOQSI010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32417_length_3320_cov_3.866462"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /gene="tsf"
                     /locus_tag="PKL42_09655"
     CDS             complement(<1..703)
                     /gene="tsf"
                     /locus_tag="PKL42_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNU67378.1"
                     /translation="MAEITASMVKELRERTDAPMMDCKRALTEAEGDMTRAEEILRVR
                     FGNKASKAAGRVAAEGTVGISISTDGKSGAMIEVNSETDFCAKNEDFLKFVNELAGVV
                     AVNKPANIEAVGALKMRDSTAEETRAQLVGKIGENITPRRFVCPQVQGKLSSYVHGSK
                     IGVIVDLVGGDDALAKDIAMHIAAAKPKSLDASGIEASLIETERRVAIEKAKEAGKPD
                     AMLEKIADGTVQKFLK"
     gene            complement(819..1565)
                     /gene="rpsB"
                     /locus_tag="PKL42_09660"
     CDS             complement(819..1565)
                     /gene="rpsB"
                     /locus_tag="PKL42_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNU67379.1"
                     /translation="MSVTMRDMLEAGVHFGHQTRYWNPKMAPYIFGDRNKIHIVNLEK
                     TLPLFEDALKHVRTLAANKGRILFVGTKRQARDIVKEEASRAGCAYVNHRWLGGMLTN
                     FKTVKQSIKRLHDFDAMLLDGSLDKFGKKEALGYKREIEKLERSIGGIKEMGGLPDAI
                     FIIDVGHESGAITEAAKLGIPVIGIVDTNNSPDGVAFVIPGNDDSSRAIRLYARGIAD
                     AVLEGRSSVITDIVKSAAEEVAPATEAEAV"
     gene            complement(2179..3246)
                     /locus_tag="PKL42_09665"
     CDS             complement(2179..3246)
                     /locus_tag="PKL42_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HNU67380.1"
                     /translation="MGKYKQLSIEERSVIQAQLTLGFKPSWIAVGLGRSVSTISRELR
                     SNGWVKLKEQPGRGRPAIAGKYRAVLAQDRADVLSAKPRVERRLKAGGILWQAVMEYL
                     KQGYSPEQIAGTLKVVNADHPNLQVSHETIYSAIYLMPRGELRTEVIGWLRFGHAKRR
                     PRARGEDRRGQIPDMVSIHDRPPEVDARLVPGHWEGDLIKGKGNRSAVGTIVERTTLF
                     TVLAKMEKATAESAVSGFSHVLNRIEAQKRLSMTYDQGKEMARHSELTQNTGVAVYFA
                     DPHSPWQRGINENTNGLLRQYLPKGEDLNIFSQEELDAIAWKLNTRPRKSLGFKCPAE
                     LFTPDAFDFRQHHAALFALQP"
BASE COUNT          853 a          812 c          623 g         1032 t
ORIGIN      
        1 cttttaagaa tttttgcaca gtaccatcag caattttttc tagcattgca tctggtttac
       61 ccgcttcttt tgctttttca attgcaacgc gacgttcagt ttctatcaaa cttgcttcaa
      121 tgccagaagc atccagtgat tttggttttg ctgcagcgat atgcatggcg atatctttcg
      181 ctaaagcgtc atcaccacca accaaatcaa cgattacccc aatcttgctg ccgtgtacgt
      241 agcttgataa tttaccttgc acttgtggac acacaaaacg acgcggtgta atgttttcac
      301 caattttacc caccaattgg gcgcgtgttt cttctgcagt gctatcgcgc attttaagtg
      361 cgccaaccgc ttcaatgttt gctggcttat taacagccac aacccctgca agttcattaa
      421 caaatttcaa gaagtcttcg ttttttgcac agaagtccgt ttctgagttc acttcaatca
      481 tcgcaccaga cttaccatcc gtgctaatgc tgatacccac ggtaccttcg gccgctacac
      541 gacccgctgc tttacttgct ttattgccaa agcgcacacg taagatttct tctgcacgtg
      601 tcatatcgcc ttcagcttca gtcagtgctc ttttgcaatc catcattggc gcatcggtgc
      661 gctcacgtaa ctctttgacc atgcttgcgg taatttcagc catttttagc tcctaaaata
      721 tctataaatc acattaatta atgtgtttat tttattgatt taattaaatt tttagtaatt
      781 aaaaaagggg cactaagccc cttttatatt aacggttatt aaacagcttc agcttctgtt
      841 gcaggggcaa cttcttcagc ggctgatttt acgatgtcag tgatcactga actgcggcct
      901 tctaacaccg catcagcaat accacgtgca taaagacgaa tggcgcgtga tgagtcatcg
      961 ttacctggga ttacaaacgc aacaccgtca ggtgagttat tggtatcaac gataccaata
     1021 actggaatgc ctagcttagc ggcttcagta attgcaccgc tttcatgacc aacgtcgatg
     1081 ataaaaattg catctggcaa gccgcccatt tctttaatac cgccaattga acgctctaat
     1141 ttttcaattt cacgtttata gccaagtgct tcttttttac caaatttgtc taagctgcca
     1201 tctagcaaca tcgcatcaaa atcatgcaag cgtttgattg attgttttac tgttttaaag
     1261 ttagtcagca tgccgcctaa ccagcgatgg ttcacatagg cacaacctgc gcgtgacgct
     1321 tcttctttaa cgatgtcacg tgcttggcgc tttgtaccta cgaataaaat acgacccttg
     1381 ttagctgcca atgtacgcac gtgtttaagt gcgtcttcaa acaaaggcaa tgttttttca
     1441 aggttaacaa tatgaatttt gttgcggtca ccgaaaatat acggtgccat ttttgggttc
     1501 cagtaacggg tttgatggcc gaagtgaacg ccggcttcta acatatcacg catagtgact
     1561 gacataagtt ctctcttttc aatgggttat gaatgcacac aacactagca acactttgat
     1621 ttaaagcatt atttttactt taaaccgaca agcacccttg aataggttgt gtgtagattt
     1681 tttaaaatga ctacgctgca aatttgcagt gcgcgcatca taccacctag caccatcgtt
     1741 taccagcatt ttgcacattt tacggacgtg aatgacagca gaaagattag gattgttaca
     1801 gcaaagcact gacttggttg cagcttgatg gtttctttca accaattggc tgtatacaac
     1861 cgcatgcaaa caaaccaaaa gcaatctaac ctcttgtatt ttatcattat aatagttggc
     1921 atacgctttg ctaatgaata atcaggcgta gatctccaat tttcgctccc gttaacccgc
     1981 aacactcatg ctgttgcggg ttttttttgc ctgcaatttg cacattagtc ctgcgtaaat
     2041 ttcttatttt aagtgtttaa aaaacgttta aattgattaa gaatttctat cgagaaaaaa
     2101 ataatgctct gcgagccttg taaataaagg cttgcagggc agccaaaaag gttgtgtgct
     2161 aatttagact aggcggtttc aaggttgaag tgcaaaaagt gcagcatgat gctgcctaaa
     2221 atcaaatgcg tctggtgtga acaactcagc agggcatttg aaccctaacg atttgcgtgg
     2281 tcttgtattg agtttccacg caatcgcatc taactcttct tggctgaaga tatttaaatc
     2341 ctcgcccttt ggcaagtatt gacgcaacaa gccattggtg ttttcgttta tcccccgttg
     2401 ccaaggacta tgcggatccg caaagtaaac tgcaacgcct gtgttctgtg ttaactccga
     2461 atgccttgcc atttctttac cttggtcgta ggtcatcgat aatcgtttct gtgcctcaat
     2521 gcgattcaac acatgactga aaccactcac agcagattca gcagtggctt tttccatctt
     2581 tgctaacacc gtaaacaggg ttgttctttc cacaatcgtt cccactgcgc tacggttgcc
     2641 tttgccttta atcaaatcac cttcccagtg tcctgggact aaacgtgcat ccacttctgg
     2701 cggcctgtca tgtatgctca ccatatcagg gatttgacca cgcctatctt ctcctcgcgc
     2761 tctcggtcta cgtttcgcat gcccaaagcg taaccagcct atcacttctg ttcgcagctc
     2821 tcctcgcggc attaagtaaa tagcgctgta aatggtctca tgcgaaacct gcaagtttgg
     2881 atggtctgcg ttcacaactt taagtgtgcc agcaatttgc tcgggtgagt agccctgttt
     2941 taaatattcc atcaccgctt gccagagaat acctccagct ttcaatcgcc tttcaaccct
     3001 aggcttcgct gacaacacgt ctgctctatc ttgcgccaac accgctcgat atttgcctgc
     3061 aatcgcaggt cgaccgcgtc caggttgctc tttgagtttc acccagccat tactgcgaag
     3121 ctctcggcta atcgtcgaaa cagacctacc caatccaaca gctatccagc taggcttaaa
     3181 ccctaatgtt aactgcgcct gtataacact acgttcttca atacttaatt gtttgtattt
     3241 tcccatgcaa cattttcctc cgaaaattgt tgcacttcaa atttgagcgc gcccaattta
     3301 aaatgattgt aaattttatt
//
LOCUS       DAOQSI010000194         3301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_32707_length_3301_cov_4.930068, whole genome shotgun sequence.
ACCESSION   DAOQSI010000194 DAOQSI010000000
VERSION     DAOQSI010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32707_length_3301_cov_4.930068"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..2715)
                     /locus_tag="PKL42_09670"
     CDS             complement(181..2715)
                     /locus_tag="PKL42_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNU67381.1"
                     /translation="MKMTVAKKMLMLTLISVVGLVGLAVFNHSQMLDVFDETNYANTN
                     IVPSMVGLDTIRFKFNTLRIRVANHTLIKEPEKMADVESAIRKLQSELADLFKNYETY
                     IADDQEKQMLEADKAGLNEYYARMEPILNASRANNKPLAHELYIAIRTPAEKANADIA
                     AHIDYKMNLGQLSAQAAATVKNRAGQLNILLSALILLATAVIGYLITCNLRKQLGAEP
                     DEVAEVADRIANGDLNSPIDLKSGDNTSLMVSMQKMQKTLKNVIDAQTVMADENKAGN
                     INAVIDADKFHGSYKTMTTNVNHMAANQADVMRKVTSCIAEFANGNFNAPLEQFPGQR
                     AFINEGVELLRSNIKTFISDMEHMSQEHDAGDIDVRIATAQYKGAYAQMAEGVNRMVA
                     GHVQEKDEMINVMKLLGDGDFLVQIQQYPGKKAEINTNLDRLKGKLKGLLDSVKWVTN
                     EHLQGNIDKSMYAEMFKGGFNELAVAINQIVAGQIELTEKALACVKEFGEGNFDAQLE
                     QFPGKKAFVNEAIEQVRSNLKALNEDAQMLANAARQGRVSVRADAARHLGDYRKIVDG
                     MNETLGMIVSPIVMVKNAVETISTASKEIAQGNADLSRRTEDQAASLEKTAASMEQLS
                     STVKQNADSAKQANQLATDASNVAIKGGDVVAEVVATMSSINESAQKIEDIISVIDGI
                     AFQTNILALNAAVEAARAGEQGRGFAVVAGEVRSLAQRSASAAKEIKELIADSVSKTA
                     EGTRQVENAGHTMQDIVTSVKRVTDIIGEIAAASLEQSSGIEQVNEAIMTMDDVTQQN
                     TALVEQAAAAAESLMEQAEELMNAVSVFTLDEENAYQKPNFRVVNG"
     gene            complement(2864..3052)
                     /locus_tag="PKL42_09675"
     CDS             complement(2864..3052)
                     /locus_tag="PKL42_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67382.1"
                     /translation="MICEEENVMENLMDYFVKTIYAVLATCVIFAMLIVFAGVNPVSA
                     AEMLPMQSSQSLPETVNA"
BASE COUNT          881 a          783 c          622 g         1015 t
ORIGIN      
        1 aataaaaatt acgctattta cagcactaca aaataacaaa aacaaccact aaaaacgctc
       61 gagagaaaat tactgctctg tgagccttta tttacaaggc ttctagagcc tctcaaaagg
      121 ttagtgcttt tttcaagcac gatttaacac tttttaatta ccttttctta cccaatttta
      181 ctagccgttc accacacgaa aattcggttt ttggtaggca ttctcttcat ctaaagtaaa
      241 tacgctgacc gcattcatta attcttcagc ctgctccatg agtgattcag ccgcagcagc
      301 cgcttgttca accagcgctg tattttgctg ggtaacatcg tccatggtca taatggcctc
      361 attcacttgc tcaatgcctg agctttgttc aagagatgcg gcggcaattt cacctatgat
      421 gtctgtcaca cgtttaacgg aagtcacaat gtcttgcatg gtgtggccag cattttctac
      481 ctgacgagtg ccttcagcag ttttgctaac ggaatcggca atcaactctt taatctcttt
      541 ggcagcgctg gctgaacgtt gcgctaagct acgtacttca cccgccacga cggcaaagcc
      601 acggccttgc tcaccggcgc gtgccgcttc tactgcggca ttcagtgcca atatattggt
      661 ttggaaggca atgccgtcaa tgactgaaat aatgtcttca atcttttgtg cactttcatt
      721 gatggagctc atggtagcaa caacctctgc gactacatcc ccccctttaa ttgccacatt
      781 tgaagcatcg gtagctaact ggttagcttg cttagcgctg tcggcatttt gcttcacggt
      841 ggatgaaagt tgctccatgc tggcggctgt tttttctaat gaggctgctt ggtcttctgt
      901 tcggcggctc aaatctgcgt taccctgcgc aatttctttt gaagctgtac taatggtctc
      961 tactgcattt ttcaccatca caatcgggct cacaatcatg cccaatgttt cgttcatgcc
     1021 atcaacaatc ttgcggtaat caccaaggtg acgcgctgcg tcagcccgca cagacacccg
     1081 gccttgccgc gcagcattgg caagcatttg cgcatcttca ttcaaagctt ttagattgct
     1141 gcgcacttgc tcaatggctt cattgacaaa tgctttttta cccggaaatt gttcaagttg
     1201 tgcatcaaag ttcccttccc caaactcctt cacacaggcc aacgcttttt cagttagttc
     1261 aatttggcca gccacgattt ggttaatagc aacagccaac tcgttaaacc cacctttaaa
     1321 catttccgca tacatggact tatcaatatt gccctgtaaa tgctcattgg tgacccattt
     1381 aacggaatct aaaagtcctt taagtttgcc tttaagccgg tctagatttg tgttgatttc
     1441 tgctttttta ccgggatact gctgaatttg cactaagaaa tcaccatccc cgagcaattt
     1501 cattacgttg atcatttcat ctttttcttg cacatgccct gctaccattc ggttcacgcc
     1561 ttctgccatt tgagcataag cacctttgta ttgcgcggtg gcaatacgca catcaatatc
     1621 accggcatca tgctcttgtg acatgtgttc catatcagaa atgaaagttt taatattact
     1681 gcgcagcaat tcaacgcctt cattgataaa tgcacgctgt ccaggaaact gctcaagtgg
     1741 cgcattaaaa ttgccattgg caaattctgc aatacagctt gtcactttgc gcatcacatc
     1801 ggcttggtta gccgccatgt gattaacatt ggtggtcatg gttttatagc tgccatgaaa
     1861 tttatcagca tcaatcaccg cattgatgtt cccggctttg ttttcatctg ccatcaccgt
     1921 ttgtgcatca atcacatttt tgagtgtttt ctgcattttt tgcattgaaa ccatcaagct
     1981 agtgttgtca ccacttttca aatcaattgg gctgttcaaa tcaccattgg caatgcggtc
     2041 tgcaacctct gcaacttcat ctggctcggc gccaagttgc ttgcgtaagt tgcaggtaat
     2101 gagataacca atcacagcgg ttgccaacaa aataagcgca cttagcaata tatttaactg
     2161 acccgcacga tttttaacag tggcagctgc ttgggcactt aactggccta aattcatctt
     2221 ataatcaatg tgggctgcaa tatcagcatt ggctttttca gcaggtgtgc gaatggcaat
     2281 atagagttca tgcgccagtg gtttgttgtt ggcgcgtgag gcatttaaaa tcggctccat
     2341 gcgcgcataa tattcattca agcccgcttt atcggcctca agcatctgct tttcttgatc
     2401 gtcagcaata taggtttcat aattcttgaa caaatcagcc agttcagatt gcagttttcg
     2461 aatggcagat tcaacatctg ccattttttc aggctcctta ataagcgtat gattggccac
     2521 ccgaatacgt aaggtattaa atttaaatcg aattgtatct agccccacca tgctgggcac
     2581 aatgttggta ttggcataat tggtttcatc aaacacatcc agcatttggc tatggttgaa
     2641 aacagccaag ccaaccaagc ccacgacaga aatcaaggtt agcattaaca tttttttagc
     2701 aacggtcatt ttcatattta acccaattca atttcttgca tcgaacaata ttccagcaat
     2761 agatgtaatt taaatcataa ccaaatgttt taattagata ataagctggc actaaagacg
     2821 ttaattcata gaattaacga ttacaattta cttaacattc accctaagca ttcacggttt
     2881 caggcaaact ttgcgatgat tgcataggca acatttcggc tgccgacact ggatttacgc
     2941 ctgcaaatac aatcaacatg gcaaatatca cgcatgttgc taatacggca taaatggttt
     3001 ttacaaagta gtccatcagg ttttccatca cattttcctc ttcacaaatc atgaccaagc
     3061 cacacaatgc ttggcacctt atgaatgtca tcggcaaaaa aaattaaaac ttaatacaat
     3121 ttgtaaaaat ttatttacaa catatggcaa tgtgagtggc tatttgacgc atttaatgtg
     3181 gattgaatat tgcatgtggg tacaacattg acagcgagaa agtaaatgct tttttaagca
     3241 gattatctcc cgagcaaaaa acattgccct gcgagccttt atttacttgg cttgcagggc
     3301 a
//
LOCUS       DAOQSI010000195         3250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_33502_length_3250_cov_4.940219, whole genome shotgun sequence.
ACCESSION   DAOQSI010000195 DAOQSI010000000
VERSION     DAOQSI010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33502_length_3250_cov_4.940219"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..579)
                     /locus_tag="PKL42_09680"
     CDS             complement(19..579)
                     /locus_tag="PKL42_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67383.1"
                     /translation="MRANTLSMQGLEEIEKQSFDLYKYPEINQFKVNQQQLRHYQIVQ
                     IATVNALNSKKIVDFETERMFASEGDKPGIKRPDVLWFTDNGKRIAVEVELSQKWDRA
                     FDEFLLAIIRSLSSKDGVKAKFDRFLIITDSNAIRHNYIEAIQPGSIFYKWEKNHRDH
                     WEVTKKLLVPDWLIQKVDFQLIKNTV"
     gene            complement(706..1518)
                     /gene="istB"
                     /locus_tag="PKL42_09685"
     CDS             complement(706..1518)
                     /gene="istB"
                     /locus_tag="PKL42_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HNU67384.1"
                     /translation="MPDILTQLKALKLHGMADSYAELVKQGASGATASLDASEWLVRH
                     LVEAESTDRAIRSIAYQMHTARFPIHRNLESFDFSESSVDQSLVVRLSSMQFTDAAEN
                     IVFVGGSGTGKSHLATALGVTGIQYHGKRVRFYSTVELVNSLEREKAAGKQGKLALSL
                     MQMDLVILDELGYLPFSQAGGALLFHLLSKLYERTSVIITTNLSFSEWSHVFGDAKLT
                     TALLDRLTHHCHIVETGNESYRFKYSSTQAKGKIKTREEIRNTFKLPEQADI"
     gene            complement(1511..3013)
                     /gene="istA"
                     /locus_tag="PKL42_09690"
     CDS             complement(1511..3013)
                     /gene="istA"
                     /locus_tag="PKL42_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HNU67385.1"
                     /translation="MVMYAKIRRMFFREHLSISEIARRTSLTRNTIKKWLKAADGTEP
                     KYRRGPAQTKLKPFESQLILALTADAHRPKRDRRTAVMLYEDIQKQGFTGCYARVTAF
                     IRQWREQSKASSKSAFVPLHFALGEAFQFDWSEEALVIGGIHRKILAAHTKLCASRAF
                     MISAYPSQSHEMLFDAHTRAFTALGGIAKRGIYDNMKTAVDKVQPGKGRIINARFFAM
                     TAHYLFDPDFCNVASGWEKGVVEKNVQDARRRVWSEAKNHQFSTFAELNAWLEVQCRQ
                     LWTRLYHTAYPQLTIADVLEQEQLYLMPMPTPFDGYTEVLARVSSTCLVTVQRNRYSV
                     PCEYANQRVSVHLYPDHISIYADHQRVAIHTRFYERDRTCYDWLHYIPIIMRKPGALR
                     NGAPFLEMPLPLQKLQLALRQRERLLGDRMMANVLTQVRNHGLEAVTIAVEQVLASGV
                     TSIEHLQNILSRLNTTAMPAPVVTSLKLDTEPLANTARYDQLNAKEKKHA"
     gene            complement(3097..>3250)
                     /locus_tag="PKL42_09695"
     CDS             complement(3097..>3250)
                     /locus_tag="PKL42_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNU67386.1"
                     /translation="LGGFLMPIMFGALLDFTGVRTSAFMLMFGIIWVSLIWMYWTVDA
                     SLKMTI"
BASE COUNT          847 a          746 c          701 g          956 t
ORIGIN      
        1 tccataagct gagtttgact aaacggtgtt tttaattaat tgaaaatcta ccttctgtat
       61 tagccaatct ggtaccaata actttttcgt cacttcccaa tggtccctat ggtttttctc
      121 ccacttataa aaaattgaac cgggttgaat tgcctcaata tagttatggc gaattgcatt
      181 gctgtctgtg atgatcagaa atctatcaaa tttagcctta acgccatctt tgctacttaa
      241 tgaacgtatg attgctaata ggaactcgtc aaatgcccta tcccactttt gtgataactc
      301 aacctcaaca gcaattcgct ttccattatc agtaaaccac agtacgtcgg gccttttgat
      361 tccaggtttg tcgccctcac ttgcgaacat tctttcagtt tcaaaatcaa ctattttttt
      421 tgagttcaga gcattaacgg tggcaatttg cactatttga tagtgtctta gctgctgttg
      481 attaacttta aattgattta tttctgggta cttatataaa tcaaaacttt gtttttcaat
      541 ttcttctaaa ccttgcatgc ttagtgtgtt ggcgcgcatt taaaattgac caggtgtgcg
      601 cgttgaattt tgaccaggcg aatttaggat gtagattact acatcgttgt ggataagtcg
      661 accaaactga cagggtctaa tgctcctttt tacgattgct ttgaattaaa tatctgcctg
      721 ttctggcagt ttgaatgtgt tgcgtatttc ctcccttgtt ttgatctttc ctttagcttg
      781 cgtactgctg tacttaaatc gataagactc attgcctgtt tctacaatat ggcaatgatg
      841 cgttaagcga tctaacaatg ctgtggtgag ttttgcatca ccaaagacat gactccattc
      901 tgagaaactt aaattagtcg tgatgatgac actggtacgt tcgtatagct tagagagtaa
      961 gtgaaacaat aaagcgcctc ctgcttgtga gaacggcaaa tagcctaatt catctaaaat
     1021 gaccaaatcc atttgcatta aactcagcgc aagtttgcct tgtttacctg cggctttctc
     1081 acgctctaaa ctgttcacca actccacggt ggaataaaag cgcacacgct tgccatgata
     1141 ctgaatccca gtaacgccta gcgcggtcgc taaatgcgac ttgcctgtgc ctgaaccgcc
     1201 cacaaagacg atgttctcag cagcatcggt aaactgcatg gatgatagcc gaacaaccaa
     1261 tgattgatcc acactcgact cgctaaagtc aaagctttcc aagttacggt gtatcggaaa
     1321 ccttgcggta tgcatttgat aagcaataga acgtatcgca cggtcagtgg attccgcttc
     1381 taccagatga cgcactaacc actcagaagc gtccaatgat gcagtagcac cacttgcacc
     1441 ttgtttaacg agttcagcat aactgtcggc cataccatgt aatttaagcg ctttaagttg
     1501 ggtgagaata tcaggcatgt ttcttctcct tcgcatttaa ctggtcgtaa cgcgccgtat
     1561 tggcaagcgg ttctgtatct aatttcaaac tggtgaccac tggggcgggc atcgcagtgg
     1621 tattcagccg acttaagata ttctgcagat gttcaatact cgtcacgcca gaggctaata
     1681 cctgctctac tgcaatcgtc accgcttcta agccatggtt gcgtacttgg gttaacacat
     1741 tggccatcat gcggtctcct aataagcgtt ctcgttgtcg caatgctaat tgtagtttct
     1801 gtagtggtag cggcatttct aaaaacggtg cgccattcct taatgcaccg ggtttacgca
     1861 taatgatggg gatgtaatgt agccagtcat aacaggttct atctcgttca taaaatcgcg
     1921 tatggatggc tacccgttgg tggtcggcat aaatactgat gtggtctgga tataagtgca
     1981 ccgagacacg ctgattggca tactcacagg ggacggagta acgattacgt tgtactgtca
     2041 ctaagcaagt gctagaaaca cgcgctaata cttcggtgta cccatcaaat ggagtgggca
     2101 tgggcattaa atacagttgc tcttgctcta acacatcagc aatggttaac tggggatacg
     2161 ctgtgtgata caagcgcgtc catagttgac ggcattgcac ttctagccaa gcattgagtt
     2221 ctgcaaaggt gctgaattga tgattcttcg cttcagacca tacccggcgg cgcgcatctt
     2281 gcacattctt ctccaccacg cctttctccc atccagaggc aacattgcag aagtcggggt
     2341 caaataagta gtgcgcggtc atggcgaaga agcgtgcgtt gatgatgcgg cctttgcccg
     2401 gctgcacctt atctaccgcg gtcttcatgt tgtcgtaaat gccacgttta gcaataccgc
     2461 ccagtgccgt gaatgcgcgt gtatgtgcat caaatagcat ctcatggctt tggctgggat
     2521 aagcactaat cataaaagca cgactggcac ataatttggt gtgtgcagcg agtattttgc
     2581 gatggattcc accaatgacc agtgcttctt cactccaatc aaactggaat gcttcgccta
     2641 atgcaaagtg cagtggtaca aaggcagatt tgctagaagc tttagattgt tctcgccact
     2701 gacgtataaa cgcagtgaca cgtgcatagc accctgtaaa cccttgcttt tgaatgtctt
     2761 catacaacat gactgcggtg cgacggtcgc gcttggggcg gtgtgcatct gcggtaagcg
     2821 ctaggataag ttgtgactca aagggtttga gtttggtttg cgctgggcct ctgcggtatt
     2881 taggctctgt tccatccgca gctttaagcc acttcttaat cgtatttctg gtcagactgg
     2941 tgcgtctggc tatctcacta atggataaat gttcccgaaa gaacatgcgt ctaatcttgg
     3001 catacatgac catggtaatc acctctttta ctcctgctta caaaataagc agaatactgg
     3061 ttttacctgg tcaaaattca acgcgcatca ctgcgcttat atggtcattt ttaaactagc
     3121 gtcaacagtc cagtacatcc aaatcagtga cacccaaata atgccgaaca tcaacataaa
     3181 ggctgaagtg cgcacccctg taaaatcaag cagtgcgccg aacataatcg gcattaaaaa
     3241 accacctaga
//
LOCUS       DAOQSI010000196         3192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_34400_length_3192_cov_3.619382, whole genome shotgun sequence.
ACCESSION   DAOQSI010000196 DAOQSI010000000
VERSION     DAOQSI010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34400_length_3192_cov_3.619382"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PKL42_09700"
     CDS             <1..495
                     /locus_tag="PKL42_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNU67387.1"
                     /translation="AAGAVAGNVWTKRQEEQKKQMEEATAGTGVAVTQTEDNRLKLEI
                     PSDISFDVGRADINPNFRTILDTFAKGLVSNPSSNVTIIGHTDSSGSDAINNPLSLNR
                     AASVRDYIVARGVATNRIAIDGRGSHEPLVANDTSANKAKNRRVEIFVAEPAPAPAPA
                     TTTN"
     gene            659..1300
                     /locus_tag="PKL42_09705"
     CDS             659..1300
                     /locus_tag="PKL42_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67388.1"
                     /translation="MFNYSFSVICGFFVLALLPNQVALAATTDGRWHPGIGDPTVFGW
                     ITVLFYAIAMCMSARLAFLQKKSGGRYQFWLFLALFLLLLGINKQLDLQTWFTQTLRE
                     AAHLQGWYDYRRPLQVLFIATLGFGMLAVLMRVRRFLADAWRLYKVTWIGIALLCSFI
                     LIRAASFHHMDVFIHHRMLGLKFNVLLEIGAILVIMAGTFIKYQVYLRSVKVS"
     gene            1418..1696
                     /locus_tag="PKL42_09710"
     CDS             1418..1696
                     /locus_tag="PKL42_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67389.1"
                     /translation="MSKLLKVLLLSLALTVGFTACKKAEETAATATEAATEAATATTE
                     AAQDAAAATTEAAGDAAAATTEAIAEAKEEAVDAVQAAKDAAAAEAAK"
     gene            complement(1765..2481)
                     /locus_tag="PKL42_09715"
     CDS             complement(1765..2481)
                     /locus_tag="PKL42_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67390.1"
                     /translation="MKNLTLIIMCLCLGACATNSPVAGKPIPERHDRSSGSQLAKSDF
                     DRMADVEYAENKQSLRTLMLKLYKRNPRELAKSTSDSAEKMVEWVFEGDAQHQYKFKE
                     INHKQGTEAIFLAFKQDFQGDRVLALMVGLQTMLLQAHDNKTDFYLTDSIDPQHIYNM
                     ARNVEIAAWKLSNARDSQGNLFLLSNEISDKGHNLSFEREFGKIIGSADLFAITLAEK
                     SQRTISRVVQNLATALFLPF"
     gene            complement(2482..>3192)
                     /locus_tag="PKL42_09720"
     CDS             complement(2482..>3192)
                     /locus_tag="PKL42_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNU67391.1"
                     /translation="NVSHELRTPLTVVGGFLETLSDMEGAIPANLKTYFTMMEEQTSR
                     MRRIIEDLLTLSTLESKTESPDDHEINMASLLTSIQNDAIGLSQSLKKAPLQIHLHVD
                     PTMNIRGAQEELRSAFSNLVSNAVRYTPAIEVINNQPNEIFISWESQNGEAVFSVRDT
                     GIGIAQAHIDRLTERFYRVDRSRSRETGGTGLGLSIVKHILIRHQAKLDISSTLDVGS
                     TFSVIFPKNRVVHISQSQ"
BASE COUNT          804 a          667 c          785 g          936 t
ORIGIN      
        1 gcggcaggcg cggtggctgg gaacgtatgg acgaagcgcc aagaagagca gaaaaaacaa
       61 atggaagagg cgacagccgg tactggtgta gcggtgacgc aaacggaaga taatcgcctg
      121 aaattagaaa ttccaagtga tatttctttt gatgtgggcc gcgctgacat caacccaaat
      181 ttcagaacaa ttttggatac ctttgccaaa ggtttggtga gcaatcctag cagcaatgtg
      241 acgattattg gccatactga tagttctgga agtgacgcaa tcaataatcc gttatcgctc
      301 aaccgtgcag caagtgtgcg cgattatatt gtggcgagag gtgttgccac aaaccgtatt
      361 gcgattgatg gccgtggttc gcatgagccg ttggtggcca atgacacatc tgccaataag
      421 gccaaaaatc gtcgtgtaga aatttttgtg gccgagcctg cacctgcgcc agcaccagca
      481 acgacaacaa attaatcaag aataacttaa gtttttaagt aaatttttta gtaaattttt
      541 gattaaccct ttttcgctgc cctgtgagcc ttgtaaataa aggcttgcaa ggcagcgaaa
      601 ttttctcggt gttttttcca gtgcttgatt gttttataat tcgtgtatgc aaaagtggat
      661 gtttaactat agttttagtg tgatttgcgg tttttttgtc ttagcgttac tgccaaatca
      721 agtcgcactg gctgcgacga ccgacggccg atggcacccc ggcattggcg acccaacggt
      781 ttttggctgg ataaccgtgc tgttttatgc aatcgccatg tgcatgagtg cgcgtttggc
      841 atttttgcaa aaaaagtcag gtggcagata tcaattctgg ctatttttgg cgctattttt
      901 attgttgctc ggcatcaata aacaacttga tttgcaaacg tggtttaccc aaacgcttag
      961 agaggccgca catttgcaag gctggtatga ttaccgacgg cctttgcagg tactgtttat
     1021 tgcaacgctc gggtttggca tgctggcagt gcttatgcgc gtgcgccgct ttttggcaga
     1081 tgcctggcgg ctttacaaag tgacatggat tggcattgcg ctattgtgca gttttatttt
     1141 aattcgcgcg gcttcttttc atcatatgga tgtatttatt catcaccgta tgcttgggct
     1201 taagtttaat gtgttgttag aaattggcgc tattttggtg attatggccg gcacattcat
     1261 caaatatcag gtgtacttac gtagcgtgaa agtttcataa accatatttt ggttgttctg
     1321 atataggtgg tggagtagaa ttcacccatc agggcggcgt tgggctaatg cgttatttta
     1381 gctgacattt ttttcattta ttaacaaagg aaactacatg agcaaattat tgaaagtgtt
     1441 attactaagc ttagctttaa cggttggttt tactgcttgt aaaaaagcgg aagaaacagc
     1501 agcaactgca actgaagctg caaccgaagc cgctactgca acgacggaag cagcgcaaga
     1561 tgctgccgct gcaacgactg aagctgccgg tgacgcagcc gctgcgacca ctgaagcgat
     1621 tgcagaagct aaagaagaag ccgttgatgc cgtgcaagct gcaaaagatg ccgctgctgc
     1681 tgaagctgca aagtaattca atttagcatt aaaaataaaa aaggagcttc ggctcctttt
     1741 ttattggggg gaaaaccaac tggattaaaa aggtaaaaac agcgcagtgg ctaaattttg
     1801 caccacacgt gaaatggtgc gctgagattt ttcagccaag gtgatggcaa ataaatctgc
     1861 actgccaata atttttccaa actcgcgctc aaacgaaagg ttgtggcctt tatcgctgat
     1921 ttcattgctg agcaaaaaaa ggttgccttg gctatcgcgc gcgtttgaaa gtttccaggc
     1981 ggcaatttca acgtttcttg ccatgttgta gatatgttgc gggtcaatgc tgtctgtgag
     2041 gtaaaaatct gttttattat catgggcttg taaaagcatg gtttgcaagc ctaccatcaa
     2101 ggccaataca cggtcacctt gaaaatcttg cttaaacgcc agaaaaatcg cttctgtgcc
     2161 ttgtttatgg ttaatttctt taaatttgta ttgatgttgc gcatcgcctt caaacaccca
     2221 ttccaccatt ttttccgcac tgtcagacgt gcttttggca agctcgcgcg ggttacgttt
     2281 gtagagtttt agcatgagcg tgcgcaggct ttgcttgttt tctgcatact ccacatcggc
     2341 catgcggtca aaatcacttt tggcaagttg gctaccagaa cttcggtcat gtcgttcagg
     2401 aattggcttg cccgccacgg gtgaattggt ggcacaagcg cctaggcaca agcacattat
     2461 aatcagagtg agatttttca tttattgtga ttggctaata tgaacaacgc gattttttgg
     2521 aaatattacg ctaaaagtac tgcccacgtc taacgtgctg ctgatgtcta atttggcctg
     2581 atggcgaatc agaatatgtt tcacaatgga taaccccaag cccgtgccgc cggtttcacg
     2641 gctacggcta cggtctacgc ggtaaaagcg ctcggtcagc cggtcaatat gtgcttgtgc
     2701 aatgccgatg ccggtgtcgc ggactgaaaa cactgcctcg ccattttgtg attcccatga
     2761 aataaaaatc tcattgggtt ggttgttgat cacctcaatc gctggtgtgt agcgcacagc
     2821 attgctgact aagttgctaa aagcactgcg taactcttct tgtgcgccgc gaatgttcat
     2881 tgtggggtca acatgcaggt gaatttgcag tggcgctttt ttaaggctct ggctcaagcc
     2941 tatcgcatca ttttggatgg atgttaacaa gctcgccatg ttgatttcat ggtcatccgg
     3001 actttcggtt ttgctttcca aggtggatag cgtcagtaaa tcttcaatga tgcgccgcat
     3061 gcggctggtt tgctcttcca tcatggtgaa atacgttttc aggttggcag gaatcgcgcc
     3121 ttccatatcg cttaaggttt ctaaaaagcc gcccaccacg gttaatggcg tgcgtaattc
     3181 atgtgaaacg tt
//
LOCUS       DAOQSI010000197         3152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_35059_length_3152_cov_4.250242, whole genome shotgun sequence.
ACCESSION   DAOQSI010000197 DAOQSI010000000
VERSION     DAOQSI010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35059_length_3152_cov_4.250242"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..491
                     /locus_tag="PKL42_09725"
     CDS             69..491
                     /locus_tag="PKL42_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose bisphosphate carboxylase small subunit"
                     /protein_id="HNU67392.1"
                     /translation="MHITQGQFSFLPDLTDAGIRLQIDYAISKGWAVSIEWTDDPHPR
                     NVYWNMWEIPMFEIKDSGAVLLALTACRKACPNAYIKINAFDNTRGVESMTMSFIVNR
                     PTMEPGFRLVRQEGEGRTIRYTTISFSAEMPPGSLCHK"
     gene            479..1402
                     /gene="cbbX"
                     /locus_tag="PKL42_09730"
     CDS             479..1402
                     /gene="cbbX"
                     /locus_tag="PKL42_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbX protein"
                     /protein_id="HNU67393.1"
                     /translation="MSQINTQINQTVDLHQILCDANITEVLEQLDLDLVGLAPVKQRI
                     KEIAAFLVVSRARLQLGIESAQPNLHMCFTGNPGTGKTTVALRMAEMLYRLGYIRKNS
                     VVSVTRDDLVGQYIGHTAPKTKEVLKKAMGGVLFIDEAYYLYRPENERDYGQEAIEIL
                     LQVMENNRDDLVVILAGYKDRMDTFFQSNPGMSSRIAHHIDFPDYTTAELLDIATKMV
                     GNMHYHLDEKAVAAMHEYIALRQAQPHFANARSIRNALDRARLRHANRVFDMASQQNV
                     NASIADLSTITEADIRASRVFNQVKKAEVYD"
     gene            1395..2456
                     /locus_tag="PKL42_09735"
     CDS             1395..2456
                     /locus_tag="PKL42_09735"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF006780.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HNU67394.1"
                     /translation="MTEHLLTLANFLSEQLKDTPQAEALNKLLVAIANATKEIATLTV
                     KGSLTNNTAKLASQNVQGETQMKLDVLANDAFIRQLKACGGVAGIVSEEMEDSLAMTA
                     GGEYLLAFDPLDGSSNVPVNVSVGSIFSVLNAPVGRAPEEADYLQAGTQQVAAGYALY
                     GPCTMLVLTVGLGTHGFTYDNEQDDFLLTHFNMQVPVETTEFAINASNARFWDAPVQR
                     YVENCQAGITGPRGKDFNMRWVASMVADVHRILIRGGVYLYPKDTKLPAKEGRLRLLY
                     EVNPMSMLVEQAGGKSSTGRQRMLDVIPDQVHQRIAVILGSRQEVTLLERYYQEFDTG
                     THNKGQSPLFSDRSLFNKQ"
     gene            2480..>3152
                     /locus_tag="PKL42_09740"
     CDS             2480..>3152
                     /locus_tag="PKL42_09740"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HNU67395.1"
                     /translation="MSRNFPVIAITGSSGAGTTSVMQSFQHVFRRENIKAQMIEGDSM
                     HRYERKEMDEELNRQKQIPGSHFSHFNPKANLLPELAETFRQFGQTGKCKVRKYIHTD
                     VEGMPYNQKAGTMTPWQDSIEGAEVLFYEGLHGGYVGEEANVAKHVDLLVGVVPIINL
                     EWIQKLHRDQKIRGYSKEAVTDIILRRMPDYVNYICPQFSRTHVNFQRVPTVDTSNPF
                     IANDIP"
BASE COUNT          912 a          662 c          763 g          815 t
ORIGIN      
        1 tctacggata ccccagactt tgttgcaacg ccaacaacct cctactaact ttaatcgaag
       61 gaattatcat gcatatcaca caaggtcagt tttcattttt acccgatctc actgatgctg
      121 gaattcggct gcagattgat tatgccatca gcaagggttg ggcagtttca attgagtgga
      181 cagatgatcc acatccgcga aatgtttact ggaatatgtg ggaaatcccc atgtttgaaa
      241 ttaaagacag tggcgctgtg ctactggcgc tcactgcttg ccgtaaagcc tgccctaacg
      301 cgtatattaa aataaatgct tttgataata cgcgtggcgt agaatcgatg accatgagct
      361 ttattgtgaa tcgcccaacg atggaacccg gcttcaggct ggtacgccaa gaaggcgaag
      421 ggcgcacgat acgctacaca accatttcct ttagcgccga gatgccacca ggcagcctat
      481 gtcacaaata aatacacaaa tcaatcaaac tgtggatttg caccagatac tgtgtgatgc
      541 aaatatcacg gaggtgctag agcagcttga tttagattta gtggggttgg cacctgtaaa
      601 acagcgtatc aaagaaatcg ccgcattttt ggtggtatct cgggcacgtt tgcaattggg
      661 catagaaagt gcacaaccta atttgcacat gtgttttaca gggaatcctg gcactggaaa
      721 aactacggta gccttgcgca tggcagaaat gctgtatcgc ttaggttata tacgtaaaaa
      781 tagcgtggta agtgtaacgc gtgatgacct agtgggccag tacattggcc acacagcgcc
      841 taaaacaaaa gaggtgctta aaaaagccat gggcggtgta ttatttattg atgaagccta
      901 ttatttgtat cgcccagaaa acgagcgcga ttacggtcaa gaagccattg agattttgct
      961 gcaagtgatg gaaaacaacc gagatgattt ggtggtgatt ttagccggat ataaagacag
     1021 aatggatacg ttttttcagt ctaaccccgg tatgtcatct cgcattgcgc accatattga
     1081 ttttcctgat tacacaacag cagaactact agatattgct acaaaaatgg ttggcaatat
     1141 gcattatcat cttgatgaaa aggccgtcgc agctatgcac gaatatattg cgttgcgtca
     1201 ggcacagccg cactttgcca acgctcgctc tattagaaat gcgctcgaca gagccagatt
     1261 acgccacgct aatcgtgtgt ttgatatggc gtctcaacaa aatgtgaatg cttcgattgc
     1321 agatttatcc accattactg aagcggatat tcgtgcttca cgcgtattta accaagtaaa
     1381 aaaggctgaa gtctatgact gaacatttat tgacgctggc taatttttta tctgagcagt
     1441 taaaagatac tcctcaggcg gaagccttaa acaagctact ggtcgccatt gctaacgcta
     1501 caaaagaaat cgcgacattg actgtaaaag ggtcgcttac caataataca gcaaagttgg
     1561 ccagccaaaa cgtacagggt gaaacacaaa tgaagctaga cgtgttggct aacgatgcct
     1621 ttattcgcca gcttaaagcc tgtggtggtg ttgcaggtat tgtttcagaa gaaatggaag
     1681 attcgctagc gatgaccgcg ggaggcgaat atttgctggc atttgatccg ctagatggct
     1741 cgtccaatgt gcctgtgaac gtgtcagtag ggtcaatttt ttcagttctg aacgcgccag
     1801 ttggccgcgc acctgaagag gctgattatt tacaagcggg cacgcaacaa gttgccgctg
     1861 gttatgcgct ttatgggcca tgtaccatgt tagtgttgac tgtggggctc ggcacacatg
     1921 gttttaccta tgacaatgag caagatgatt ttttgttaac gcatttcaat atgcaagtgc
     1981 cagttgaaac aaccgagttt gcaatcaacg ccagcaatgc aaggttttgg gatgcgcccg
     2041 tgcaacgtta tgtcgaaaac tgtcaggcgg gtatcacagg tccacggggc aaagatttta
     2101 atatgcgctg ggtggccagc atggtggcag acgtgcatcg aattttaatt cgcggtggtg
     2161 tgtatcttta cccaaaagac actaagttgc cagcgaaaga aggtcgatta cgcttgctgt
     2221 atgaggtgaa cccgatgagt atgctggtag agcaggcagg aggaaaaagc tcaacagggc
     2281 ggcagcgtat gttggatgtt attcctgacc aagtgcatca gcgcattgcg gtcatattag
     2341 gttctcgtca agaagtaacg ttacttgagc gctattacca agaatttgat actggaactc
     2401 acaataaagg ccagtcacca ttattcagtg accgctctct ctttaataag cagtaaaaaa
     2461 tataaaaaag ggaatagata tgtctagaaa ttttcctgtt atcgccatta ccggttcttc
     2521 tggtgcaggg acgacttcag tgatgcaaag ctttcagcat gtgttcaggc gtgaaaatat
     2581 caaagcgcag atgatagagg gcgactcgat gcaccgctat gagcgcaaag aaatggacga
     2641 ggaactcaat cgtcagaaac aaattcccgg cagtcacttc agccacttca accccaaagc
     2701 caatttgctg cctgaattgg cagaaacctt tcgtcagttt ggtcaaacag gaaaatgtaa
     2761 ggtgcgtaaa tatatccaca cggatgtaga aggcatgcct tacaatcaaa aagcaggcac
     2821 catgacacct tggcaagaca gcatagaggg cgcagaagta ttattttacg aaggcctgca
     2881 tggcggttat gtgggtgaag aggccaatgt agcgaagcat gtagatttgc tggtaggcgt
     2941 ggtgccaatt attaaccttg agtggataca aaaactgcat cgcgaccaaa aaatacgcgg
     3001 ttattctaaa gaggcagtga ccgatattat tttgcgccga atgccagatt atgtgaatta
     3061 tatctgcccg cagttttcgc gcacacatgt caattttcag cgtgtgccaa cggtagatac
     3121 ttctaatcca tttattgcaa atgacattcc aa
//
LOCUS       DAOQSI010000198         3120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_35632_length_3120_cov_4.239804, whole genome shotgun sequence.
ACCESSION   DAOQSI010000198 DAOQSI010000000
VERSION     DAOQSI010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35632_length_3120_cov_4.239804"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PKL42_09745"
     CDS             complement(<1..280)
                     /locus_tag="PKL42_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HNU67396.1"
                     /translation="MNHISKRLNLTLQKICSHCLHCLLIASVIYSPNTQAERLKDIAT
                     IQGVRSNQLLGYGLVVGLDGTGDQTTQTPFTVQSILSMMQQMGVSLPPG"
     gene            complement(293..976)
                     /locus_tag="PKL42_09750"
     CDS             complement(293..976)
                     /locus_tag="PKL42_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HNU67397.1"
                     /translation="MKNLMVKTIANVVHSVTCLALLSLAACAITPDSIVKQPTTAKPQ
                     PVVANAPKTGAIFNNGAYKPLFEDRRARFVGDILTITIAENTSATKANGDSGSKDGKA
                     EGGISKLLGKTFPKTEFSASSSTSYEGKNAANASNVFNGSVTATVVEVLSNGNLVVSG
                     EKQVSFDKSTEYVRFSGVVNPDTITAGNMVTSTKIADARIEYRTSSKIDASEIASLFA
                     RFFLSMAAL"
     gene            complement(1033..1815)
                     /gene="flgG"
                     /locus_tag="PKL42_09755"
     CDS             complement(1033..1815)
                     /gene="flgG"
                     /locus_tag="PKL42_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HNU67398.1"
                     /translation="MMRSLWISKTGLDAQQTQMDVIANNLANVSTSGFKRSRAVFEDL
                     IYQTIRQPGAQSSQQTQLPSGLQLGTGVRPVATERIFTQGNLQLTSNDKDAAIQGAGF
                     FQVLLPDGSTAYTRDGSFQTDSQGQLVTSSGFPLQPAITIPANAQSLTIGRDGTVSVT
                     TPGASAPTQVGAIQLATFINPAGLQSKGENLYVETSASGNANTNAPGANGAGLLTQGY
                     VETSNVNVVEELVNMIQTQRAYEINSKAITTSDQMLQKLTQM"
     gene            complement(1869..2609)
                     /gene="flgF"
                     /locus_tag="PKL42_09760"
     CDS             complement(1869..2609)
                     /gene="flgF"
                     /locus_tag="PKL42_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HNU67399.1"
                     /translation="MDRMIYTAMTGAKHILEQQATTAHNLANVTSTGFKAQVDSFRAV
                     PVLSTGLPTRAFVVDATVGADFNPGALQQTGRDLDVAVHGNGWLVVQRADGSEGYTRN
                     GSLKISENGVLQTANGLTVMGDGGPISIPPDITISIGKDGTISSVSNSTLPGPSNALG
                     RLKLVNPEESNLKRADDGLFVTKNGTPAEADANVQVVNGALESSNVNVVDAMVTMISL
                     ARQFEMQMKLVQSAENNANKASQLLNMS"
     gene            complement(2680..>3120)
                     /locus_tag="PKL42_09765"
     CDS             complement(2680..>3120)
                     /locus_tag="PKL42_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HNU67400.1"
                     /translation="VALTMDFAGSTQFGSNFSINSLSQDGFTSGRLASFNTGKDGTIV
                     GRYTNGQSKTLGQVVLANFVNPNGLQSLGGNAWVESADSGVPLIGTPNTGSMGVLQSS
                     AVEDSNVDLTAELVNMITAQRVYQANAQTIKTQDQVLQTLVNLR"
BASE COUNT          784 a          694 c          712 g          930 t
ORIGIN      
        1 tgcctggtgg caaactcacg cccatttgct gcatcatact taatatgctt tgtacagtaa
       61 aaggtgtttg cgtggtttgg tcgcccgtgc catccaaacc gacgaccaag ccgtacccaa
      121 gcagctgatt actccgtact ccctgaatgg ttgcaatgtc ttttaaccgc tccgcttgtg
      181 tattgggtga ataaattaca ctggcaatca gcagacaatg caggcaatgg ctgcaaattt
      241 tttgtagcgt taaatttaat cttttgctga tatggttcat attgttgatg tgctagagcg
      301 cagccatact taagaaaaag cgtgcgaata atgaggcaat ttcagaggca tcaattttgc
      361 tgctggtgcg atactcaata cgcgcatctg caatttttgt ggaggtcacc atgttgccgg
      421 ccgtaatggt gtctggattt acaacaccag aaaagcgcac gtattcagtg cttttatcaa
      481 aggaaacttg tttttcacct gacacgacta aattgccgtt ggataatact tcaacaactg
      541 tagcggtgac ggagccatta aatacattgc tcgcatttgc cgcgtttttg ccttcatatg
      601 aagtggatga actcgcacta aattcggttt taggaaaagt tttgccaaga agtttgctga
      661 taccaccttc agctttgcca tctttgctgc cagagtcgcc gttagctttg gttgcactgg
      721 tattttcagc aatggtgatg gtgaggatat cacccacaaa gcgcgctctg cggtcttcaa
      781 acaagggttt ataagcaccg ttattgaaaa ttgcgcccgt ttttggcgca ttggcaacaa
      841 ctggctgcgg ttttgctgtt gttggctgtt ttacaatgga gtcaggtgtg atggcacagg
      901 cggcaagact tagcaaggct aagcaagtaa cactatgcac tacattagct atcgttttca
      961 ccatcaaatt tttcatttta gtcatttagt taaacttttg ttctatcaaa ttggattgat
     1021 ataatgccag ccttacattt gcgtcaattt ttgtaacatt tggtcagagg ttgtaatggc
     1081 cttactgtta atttcataag cacgttgtgt ttgaatcata ttgaccaatt cttctactac
     1141 attgacgttt gacgtttcaa cgtaaccttg agtgagtaaa cctgcaccat ttgcaccggg
     1201 tgcgttggta ttggcattgc cagaagcaga agtttcaacg tagaggtttt caccttttga
     1261 ctgcaagccg gctgggttta taaatgtcgc caattgtatc gcgcccactt gtgtaggtgc
     1321 tgatgcacct ggtgtagtga cagaaaccgt gccatcacga ccaattgtta agctttgtgc
     1381 attggccgga atggtaatcg caggttgtaa tggaaaaccg cttgatgtga caagttgacc
     1441 ttggctatca gtttgaaatg agccatcgcg cgtgtaggct gtgctgccat caggtaataa
     1501 cacttgaaag aagcctgcac cttgaatggc tgcatcttta tcattgctgg tgagctgtaa
     1561 attaccttgc gtaaaaatgc gttcagtggc aacgggtcta acgccagtgc caagttgcaa
     1621 gccagaaggc aattgtgttt gctgtgaaga ttgtgcacca ggctggcgaa tggtttggta
     1681 aatcaagtct tcaaataccg cacgtgagcg tttaaagcca ctggtgctga cgtttgccaa
     1741 gttgttggca atcacgtcca tttgcgtttg ttgcgcgtct agccctgttt ttgaaatcca
     1801 tagtgaacgc atcattttgt aattcctttc agaaatctgt cattaagtgc atattaacgt
     1861 aagcgcactc aactcatatt gaggagttga ctggctttat tggcgttatt ctcagcgctc
     1921 tgtactaatt tcatttgcat ttcaaattgg cgcgcaaggc taatcatcgt gaccatcgca
     1981 tctaccacat taacgttgct gctttctaat gcaccattca ctacttgaac gttggcatct
     2041 gcttctgcgg gcgttccatt tttggtaaca aacaagccat catcagcgcg ctttaaattg
     2101 ctttcttctg gatttaccag ttttaatcgg ccaagtgcgt tggaagggcc aggcagtgtt
     2161 gaatttgaaa ctgaagaaat cgtaccgtct ttaccaatag aaatcgtaat atctggcgga
     2221 atgctaattg ggccaccatc gcccatgacc gttaagccat tggctgtttg tagcacgcca
     2281 ttttcactga ttttaagtga gccgttgcgc gtataacctt ctgaaccatc tgcgcgctgt
     2341 acaaccaacc agccattacc atgcaccgcc acatccagat ctcggccagt ttgctgaagt
     2401 gcaccagggt taaagtccgc accaaccgtt gcgtccacca caaatgcacg cgtaggcaaa
     2461 cctgtgctca acactggcac agcgcgaaat gaatctacct gtgccttaaa gccggtagaa
     2521 gtgacgtttg caaggttatg tgcagttgtg gcttgctgct caagtatatg ttttgcgcca
     2581 gtcatcgcgg tataaatcat gcggtccatc taggctctcc taacatgcta ttaagctaaa
     2641 ttgctattaa gtaagtgcat tcagtcaatg atgcttaaat tagcgtaaat tcaccagtgt
     2701 ttgcagcact tggtcttgtg ttttaatggt ttgtgcattg gcttgataca cacgttgtgc
     2761 ggtaatcatg ttgactaact cagccgtcaa gtcaacgttg gaatcttcaa ccgcagacga
     2821 ttgcaacaca cccatagagc cagtattagg tgtgccaatt agagggacgc cagaatctgc
     2881 actttcaacc caagcgttgc cccccaatga ttgcaagcca tttggattga caaaattagc
     2941 cagcactact tgccccaatg tttttgattg gccattagtg tatcggccca caatggtgcc
     3001 atctttaccg gtattaaagc tggctaatcg gccagaagta aagccgtctt gtgacagact
     3061 attgatgctg aagttagagc caaattgcgt gctaccggcg aagtccatgg ttaatgcgac
//
LOCUS       DAOQSI010000199         3116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_35716_length_3116_cov_4.879451, whole genome shotgun sequence.
ACCESSION   DAOQSI010000199 DAOQSI010000000
VERSION     DAOQSI010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35716_length_3116_cov_4.879451"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="PKL42_09770"
     CDS             <1..772
                     /locus_tag="PKL42_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HNU67401.1"
                     /translation="LNVKLNQRIIALPHVKELFVQPASGDNGTSLGAATYAAHLAGDK
                     IQKMEHAYLGPAFTTEECIAACEAHPAKPIFTRVENAAQKGAELLASGNPLAWLQGRM
                     EFGPRSLGCRSILGDPSYPGVADRINAQIKYRERWRPFCPSMLDRVAEDILQTAHPAP
                     YMTFTFDVAEKWKSRIPEVVHEDGTARAQVVTRETNPRYYELIEHLEKLRGNPVVLNT
                     SLNRRGEPMICSPTDALNMFYGCDLEYLMMEDVLVTKK"
     gene            979..2793
                     /gene="edd"
                     /locus_tag="PKL42_09775"
     CDS             979..2793
                     /gene="edd"
                     /locus_tag="PKL42_09775"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HNU67402.1"
                     /translation="MKQSHIEVTERIIEKSRPTRTAYLQRVDAMINRPKSTGRLGCAN
                     LAHAYAAMPSDDKFKVVVEKAPNIGIVTAYNEMLSAHQPYVGYPDIIRNEAHKLGATV
                     QVAGGVPAMCDGITQGEPGMELSLFSRDTIAMSTAIALSHDVFDAALLLGICDKIVPG
                     LLIGALHFGHLPCVFVPAGPMATGIDNTTKSKVREKYAQGLVGRDELLASESAAYHSA
                     GTCTFYGTANSNQMIMEAMGLHVPGAAFVHPNDGLRELLTREAVKMVLNNTKQEQFTP
                     IGKLVDEHVIVNAMVALLATGGSTNHLIHWVAVARAAGIMIDWTDFYHLAKTTPLLAS
                     VYPNGKADVNEFQSAGGPAFVIRELIDAGYMFPDVYTVAYGGLRDYGKLPVKEENKLV
                     WKDLPKESLDETIVRTAAFPFDESGGLRLLKGNLGRSVIKISAVPPDRHIIEAPAIVF
                     DAQEELLAAFDRGELEKDFVAVVRFQGPKANGMPELHKLTPPLAVLQNKGFMVAIVTD
                     GRMSGASGKIPAAIHMTPEASAGGAIAKIRTGDIVRLNATVGMVNVLVDEDEWAEREV
                     EALSDTKRHHNAHDLGRELFGGMRKNVLSAEEGAITWL"
BASE COUNT          803 a          670 c          819 g          824 t
ORIGIN      
        1 gctgaatgtg aaattgaatc agcgcattat tgcgttgcca catgtgaaag aattgtttgt
       61 gcagccagcc tctggtgata acggaacttc gttgggtgcg gctacttatg cggcgcattt
      121 ggcaggcgat aaaatccaaa aaatggaaca cgcgtatttg ggaccagcat ttaccaccga
      181 agagtgtatt gccgcctgtg aagcgcaccc agcaaaaccc atatttacac gtgtagaaaa
      241 tgccgcacaa aaaggtgcag aattgctggc cagtggcaac ccattagctt ggttgcaagg
      301 acgtatggaa tttggcccac gctcgcttgg ctgccgcagc attttgggcg acccaagtta
      361 ccctggtgtg gcagaccgta ttaacgcaca aattaaatat cgtgaacgct ggcgtccatt
      421 ttgccccagt atgctagacc gcgtggcgga agatattttg caaaccgcac atcccgcgcc
      481 ttatatgacg tttacctttg acgtggcaga aaaatggaaa tcgcgtattc ctgaggtggt
      541 gcatgaagat ggcaccgcgc gtgcgcaagt ggtaacgcgc gaaaccaacc cgcgttatta
      601 tgagcttatt gaacatcttg aaaagctgcg tggtaatcct gtggtgttga atacctcact
      661 taatcgccgt ggtgagccga tgatttgctc acccaccgat gcgctcaaca tgttttacgg
      721 ctgtgattta gagtatttaa tgatggaaga tgtgctggta acaaaaaaat aggttgttag
      781 caccggcagg cagcattgga gcattgcaaa tacatttgca actgaacccg tgccgcacgc
      841 gcttttttgc tagttgattt agcgcaactc tgtgtgacta atgctcgtgt tagatttgac
      901 gtaaagcgcc taattagcga taatcagggt gaaggtttta gccccattga agcaccatct
      961 taagttgaag gcctagtcat gaaacagtcc catattgaag ttaccgaacg catcatagaa
     1021 aaaagtcgcc caactcgcac tgcgtatttg caacgagttg atgcgatgat taatcgcccc
     1081 aaaagtacag ggcgcttagg ctgtgcaaat ttggcgcatg cctatgcagc aatgcccagt
     1141 gatgataagt tcaaggtggt cgtcgaaaag gcgccaaaca ttggcattgt aacggcttac
     1201 aatgaaatgc tttctgcaca ccaaccttat gtgggttacc ccgatattat tcgtaatgaa
     1261 gcgcataagt taggggctac tgtacaggtg gcgggcggcg tgcctgccat gtgtgatggc
     1321 attacccaag gcgagccggg catggaactg tcgttattta gtcgcgacac aatcgccatg
     1381 agtacggcca ttgccctttc acacgatgta tttgatgccg cattgttgct tggcatttgc
     1441 gataaaatcg tcccgggttt gttgattggc gcactgcatt ttggtcattt gccctgtgtg
     1501 tttgtgcctg ctggcccaat ggcaacaggc attgataaca ccacaaaatc aaaagtgcgt
     1561 gaaaaatatg cacaagggct ggtggggcgc gatgagttgt tagcgtctga atcggccgct
     1621 tatcatagtg ccggaacctg tacgttttat ggcacagcca atagcaatca aatgataatg
     1681 gaagccatgg gcttgcatgt accaggggcg gcgtttgtac accccaatga tggtttacgt
     1741 gaattattga cgcgtgaagc cgttaaaatg gtgctcaata acaccaaaca agaacaattt
     1801 acgccgattg gtaaattggt ggatgagcat gttattgtga atgcgatggt agctttgttg
     1861 gctacgggtg gctcgaccaa tcacttgatt cactgggtag cggttgcgcg tgcggcaggc
     1921 atcatgattg actggacaga tttttatcac ttggcaaaaa ctacgccatt attagccagc
     1981 gtttacccga acggtaaagc cgatgtcaat gaattccagt ctgctggcgg ccctgctttt
     2041 gtgattcgag aacttatcga cgcaggttat atgttcccag atgtgtacac tgttgcttac
     2101 ggcgggttgc gcgattacgg taaattgcca gtaaaagaag agaataagtt agtgtggaag
     2161 gatttaccca aagaaagtct ggatgaaacc attgtgcgta ccgcagcgtt tccgtttgat
     2221 gagtcaggcg gtttgcgttt gctgaagggt aatctgggcc gtagtgtcat taaaatttct
     2281 gccgtgccgc cagatcgcca tattattgaa gcgcctgcca ttgtgtttga tgcgcaagaa
     2341 gaattgctcg cagcttttga ccgtggtgag ctggaaaaag attttgtggc cgtggtgcgt
     2401 tttcaagggc caaaagccaa tggcatgcct gaactgcaca agttaactcc gccattggca
     2461 gtgctgcaaa acaaaggctt tatggtggcg attgtgacag acggccgcat gagtggtgct
     2521 tcaggcaaga ttcctgccgc gattcacatg acgcctgaag cttctgcagg tggggcgatt
     2581 gcaaaaatcc gtaccgggga tattgtccgc ttaaatgcga ccgtgggcat ggtgaacgtg
     2641 cttgtggatg aagatgagtg ggctgagcgt gaagttgaag cgctttcaga taccaagcgt
     2701 caccacaatg cgcatgattt agggcgtgag ttgtttggtg gcatgcgtaa gaatgtgttg
     2761 tcagctgaag aaggcgccat cacttggctt tgatgttttt gaaatcacgc gataactgcg
     2821 ttgcgctcac ttgccgtact catcgtactg tctgcgtttg cgcgccttgt tctcacgcaa
     2881 tttgaaaaac atcttgttac tgctcaagtg ccttgccttg tgattttgaa acggcgcgtt
     2941 taagtataat tacgacgaaa acaaagcaaa attgcaaact taatattccc tcgccctttg
     3001 ggagagggct agggtgaggg taaaatctca cttattaaaa aatttaaaat atttttcatc
     3061 aataggtaaa aaccatgaat acattagatt tagcaaaaga aggccccgtc attcca
//
LOCUS       DAOQSI010000200         3106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_35870_length_3106_cov_4.574893, whole genome shotgun sequence.
ACCESSION   DAOQSI010000200 DAOQSI010000000
VERSION     DAOQSI010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35870_length_3106_cov_4.574893"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="PKL42_09780"
     CDS             <1..775
                     /locus_tag="PKL42_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNU67403.1"
                     /translation="GGVGPHFDSYDVFLLQGQGQRLWRISEQTDLSLIEGAPLRILKH
                     FDTAQEWLLEAGDMLYLPPHLAHWGIAVSDGKTDCMTYSIGFRAPKQQELASEFLGFM
                     QDKINQETTAYTGVYQDPDLSLQEHPAEISNDMVNKVSSMLKTIQWSNLDIATFLGTY
                     LSEPKADVVFVANKKLKLATFSEKVAKSGLRLDLKSQMLFFSNHFFINGEWADFFGEP
                     AECLKTLANHRSLDAISIEDDKLLQQLYDWYIVGYINIR"
     gene            800..1297
                     /locus_tag="PKL42_09785"
     CDS             800..1297
                     /locus_tag="PKL42_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67404.1"
                     /translation="MNNYAALAPNQIIAGERLYEEAIDLILASAQNELLIFDQDLRQG
                     DFASLKKFEFFQRFLSDNLNSRLTIILQNTAFFQEKCPRLYKLLSIYGHKMMVYETNN
                     TAKHAKDCFILADQQHYIKRIHIDQARFKYAFNDAESVLQLQSRFQDLLEATETTVSV
                     TRLGL"
     gene            1299..2153
                     /locus_tag="PKL42_09790"
     CDS             1299..2153
                     /locus_tag="PKL42_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNU67405.1"
                     /translation="MSASTLRRFSASLCFLFAWFLFAMLFSITAMAATEFKIITLQHR
                     FADDLLPIIQPLAGEDGVVTGMQNQLIIRTSPENMAEIEQVIASMDVARSNLKITVNR
                     QSNLDSTQHGVDVSGRKRIGNVAISTNRYPRQARDGVQIDIQNNSSTTKQNSQQFINV
                     ADGEFAFIQVGQSVPFTQEWRTFAHRYTHIQRTTEFIEVSTGFAVRPRSIGGHIELDI
                     TPRIAQLNQQGFIDFEALRTVVRVNKGEWFDLGGAMQRNDEVSRAILSLKNKAQTQNN
                     VLNIRVDD"
     gene            2351..2659
                     /locus_tag="PKL42_09795"
     CDS             2351..2659
                     /locus_tag="PKL42_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67406.1"
                     /translation="MTRSALLAALLALALTACGEKAPEAAATDAAAAVTEAATDAAAA
                     TTDAAATAAAATTEAATDAAAATTDAAATAAAATTEAATDAAAAVTEAAAPAADAAAH
                     "
     gene            complement(2902..>3106)
                     /locus_tag="PKL42_09800"
     CDS             complement(2902..>3106)
                     /locus_tag="PKL42_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNU67407.1"
                     /translation="GHLENLDAAQLLSKLNHQQLRHIVAAHLSAKNNTPKLAQSALAK
                     VLGCEEDFIGIADQVLGFDWRTI"
BASE COUNT          904 a          691 c          639 g          872 t
ORIGIN      
        1 tggcggtgtg ggcccacatt ttgatagcta cgatgtgttt ttgctccaag gccaaggcca
       61 gcgtttatgg cggattagcg agcaaaccga tttaagcctc attgaaggtg cacctttgcg
      121 tattttaaag cactttgaca ctgcacaaga gtggttacta gaagcaggcg atatgcttta
      181 tttaccccct catttggcac attggggcat tgccgtatca gacggcaaaa ctgattgcat
      241 gacgtattcc atcggcttta gagcgcccaa acagcaggaa cttgccagtg agtttttagg
      301 ttttatgcaa gataaaataa accaagaaac aactgcttac acgggtgttt atcaagaccc
      361 tgatttaagc ctgcaagagc acccggccga aatcagcaac gacatggtga acaaagtcag
      421 ttccatgcta aaaacgattc agtggtcaaa cttggatatt gctacttttt tagggactta
      481 tctttctgaa cccaaggctg acgttgtgtt tgtagccaac aaaaagctaa aactcgccac
      541 ctttagcgaa aaagttgcaa aaagtggctt gcgccttgat cttaaaagcc agatgttatt
      601 tttttcgaac cactttttta tcaacggtga atgggctgac ttttttggcg aacctgccga
      661 gtgccttaaa acattagcaa accatcgcag cttagacgca ataagcattg aagatgacaa
      721 gttgcttcaa caactttatg attggtatat tgtcggttac attaacattc gctaacttgt
      781 ttgctgatta aaatacgtta tgaacaatta cgccgcatta gcacctaatc aaatcattgc
      841 aggcgagcgc ctttatgaag aggccattga cctcatttta gccagcgcgc aaaacgaact
      901 actgattttt gaccaagatt tgcgacaggg tgactttgcc agcctaaaaa agtttgagtt
      961 ttttcaacgc tttttaagtg acaacctcaa tagccgttta accattattt tgcaaaacac
     1021 cgcgtttttt caagaaaaat gcccgcgact gtataagtta ctgtcaattt acggtcacaa
     1081 aatgatggta tatgaaacca ataataccgc caaacacgcc aaagactgct ttattttggc
     1141 ggatcagcag cattacatta agcgcattca tatcgaccaa gcgcgcttta aatatgcctt
     1201 caatgatgca gaatctgttt tacaactgca atcacgcttt caagatttac ttgaagccac
     1261 tgaaaccacg gtatctgtaa cgcggcttgg tctttaatat gtccgcttca acattacgtc
     1321 gctttagtgc cagtctatgt tttttatttg cgtggttttt atttgccatg ctgttttcaa
     1381 tcacggcaat ggctgccacc gagtttaaaa ttattacctt acagcaccgc tttgcagatg
     1441 atctgctgcc tattattcag cctttagcgg gtgaggatgg cgtggtcact ggcatgcaaa
     1501 atcagctaat tattcgtacg agtcctgaaa acatggccga gattgagcaa gtcattgcca
     1561 gtatggacgt ggcgcgtagc aatttaaaaa tcaccgtgaa ccgacaaagc aatttagatt
     1621 ccactcaaca cggcgtagac gtgagcggcc gcaaacgcat tggcaatgtg gcgattagca
     1681 ccaaccgcta cccaaggcag gcgcgggatg gtgtacaaat agatattcaa aacaacagca
     1741 gcacaacaaa acagaatagc cagcaattta tcaatgtggc ggatggcgaa tttgcattta
     1801 ttcaggtggg gcaatctgtg ccgtttacgc aagaatggcg tactttcgca caccgctata
     1861 cgcatattca acgcaccaca gaatttatcg aggtttcaac tggctttgcc gtacgtcccc
     1921 gtagcattgg tgggcatatt gaactcgaca ttacacctag gattgcacaa ctcaaccagc
     1981 aaggctttat cgattttgag gcgctacgca ccgtggtgcg cgtcaataaa ggtgagtggt
     2041 ttgatttagg cggcgccatg cagcgaaatg atgaagtgag ccgcgccatt ttaagcttaa
     2101 aaaacaaagc gcaaacacaa aataatgtgc tcaatattcg ggtagacgac taaaaagttg
     2161 taaaaataag acattttctc agctttattt gctaactctt taaaaataat cactttattt
     2221 taaaaattta ctggttttat ttaaaattac tggtagaatt cgcgccactc ggtatagcgt
     2281 ttaaggacaa ccttatagca agtatcgaaa taagtttttt aactcgtgat ttaaacctta
     2341 aggaaataaa atgacacgtt ctgctctact tgctgcatta ttggctcttg ctttaactgc
     2401 ttgtggcgaa aaagcgcctg aagctgctgc tactgacgct gctgctgctg taactgaagc
     2461 tgcaactgac gctgccgctg caacaactga cgctgctgct actgctgctg ctgcaactac
     2521 agaagctgca actgacgctg ctgctgcaac aactgacgct gctgctactg ctgctgctgc
     2581 aactacagaa gctgcaactg acgctgctgc tgccgtaact gaagctgctg ctccagctgc
     2641 tgacgctgct gctcactaag taactttagt aaaaaacaaa aaagccgact tttagtcggc
     2701 ttttttttgg tcttattcac ggccatgttt acaaaaattc cattcaaaat tcagtcaatt
     2761 aaatggctca ttgtcagtca accaaacact tcaaattgac attaaaattg ttaaatttac
     2821 cgccttaaaa cctacgagaa aaaaataatg ctctgcaagc ctttatttac aaggctcaca
     2881 gagcatcaaa aaaggtttgg cttaaattgt gcgccaatca aagcctaata cttggtctgc
     2941 aatgccaata aaatcctcct cacaaccaag cacttttgcc aaggctgatt gcgctaattt
     3001 tggcgtgtta tttttggcac ttaaatgcgc ggccacaata tggcgtaatt gctgatgatt
     3061 taattttgaa agcaactgcg cggcatctaa attttccaaa tgccct
//
LOCUS       DAOQSI010000201         3101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_35969_length_3101_cov_4.909389, whole genome shotgun sequence.
ACCESSION   DAOQSI010000201 DAOQSI010000000
VERSION     DAOQSI010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35969_length_3101_cov_4.909389"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1771)
                     /gene="ycaO"
                     /locus_tag="PKL42_09805"
     CDS             complement(<1..1771)
                     /gene="ycaO"
                     /locus_tag="PKL42_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HNU67408.1"
                     /translation="MTTHNTPNDSNPHETFIPSKDRSLESSILTLQGKLKALGIEVEE
                     KSWLNEIEGIWSVHVIDRDCPRIFTNGKGASQLAARASALGEYFERLSTNYFWTHFYL
                     GKNVANNDFVHYPNEQWIKLDGDRWPTALLTPELQQFYNPEGTTVASQLIDLNSGNSA
                     RGICAIPYKRLRDDQTVLFPVNLIGNLYVSNGMSAGNTLMEARTQALAEIFERDIKYK
                     IIREGLCLPDVPESVINRYPRIAAGIKGLRAAGYGILIKDASLGGKYPVMNVTLLHPD
                     DQGCFASFGAHPRFEVALERALTELLQGRAIDGLTGFAAPGFDMEEIADSQNLEIHFV
                     DSSGVISWDFLRDTPDFAFTDWNFSSTTQEDYDWCVNTIHASGHDMYIADFTHLGVYA
                     CRIFVPGMSEIYPVEELEWENNTVGNLVRPFALKLQDLNPVESNALLETLLDLDLAEE
                     LPVTTLIGLCADPNTTWADLRVGELKTLAALASGRIEETLDGCAWLAQFNEITPLRAS
                     VYRCVANILQLDAELSGSAHYEKNLQLMYGKDVLQQAQQLIQQKAQYFGLNNLGDNME
                     GSLMHQKLLAAYEKLRLHKIAAYN"
     gene            1946..>3101
                     /locus_tag="PKL42_09810"
     CDS             1946..>3101
                     /locus_tag="PKL42_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNU67409.1"
                     /translation="MSTHHIQSMRNQFYKAFMVLTLSLSITACVSVSNQQNVAKEQDW
                     PSYGRDYTNQRFSPLTQINPQNVKNLTLAWHFKSGVSASFQATPIVVGGIMYISLPYN
                     HVVALDAKTGQERWRYQHERRAQWKMCCGPANRGVAVNNGKVYIGTVDARLIALDAKN
                     GQKLWDIDVADDTALTENASSLSHSDAKSKKDIYGGTGIGINMAPVVYKGKVIIGVTG
                     VGYGLHLDTPTQDAPLGAVVGVDGRYGRPGFLAAYDALTGKRIWQFETIPSQGWEGEF
                     AETTSDGISLNRDIASEKANLQNNQDAWRYGGGSAWSTPAIDTQTNTLFFGTGNPSPQ
                     MNDISRPGDNLYTVSLVALDTETGKLKWYYQQVPHDVWGYDLASPPVLFNY"
BASE COUNT          849 a          696 c          701 g          855 t
ORIGIN      
        1 agttgtaagc cgcaatttta tgcaaacgaa gcttttcgta ggcggctaat agtttttgat
       61 gcatgaggct tccttccata ttgtcaccta aattattcag gccaaaatat tgcgcttttt
      121 gttggataag ttgctgtgcc tgttgcaaca catctttgcc atacatcagt tgcagatttt
      181 tttcataatg tgcgctgccg ctaagctcag cgtctaattg caagatattg gcaacacagc
      241 ggtaaacgct agcacgtaat ggcgtaatct cgttaaattg cgctaaccag gcacagccgt
      301 ctaatgtttc ttcaatgcgt ccgcttgcta atgcggcaag tgttttaagt tcgcccacac
      361 gtaaatcagc ccaagtggtg tttgggtcag cacataagcc aatcagcgtg gtcacgggta
      421 actcttctgc caagtctaaa tccagcaagg tttctagcaa cgcattcgat tcaacaggat
      481 tcaaatcttg tagctttaat gcaaaagggc gcaccaggtt gcctacggta ttgttttccc
      541 attctaattc ttctaccggg taaatctcac tcatgcccgg cacaaaaata cggcaggcat
      601 acacgcccaa atgtgtgaag tcggcaatat acatgtcgtg gccgctggca tgaatcgtat
      661 tcacgcacca gtcataatct tcttgggtcg ttgaactgaa attccagtct gtaaatgcaa
      721 agtctggcgt atcgcgcaga aaatcccaac taatcacgcc gcttgaatcc acaaaatgaa
      781 tttccaagtt ttgcgagtca gcgatttctt ccatatcaaa gccgggggct gcaaagccgg
      841 ttaaaccatc aatggcacgg ccttgcaata gttcggtgag tgcgcgttct agcgccactt
      901 caaagcgcgg atgcgcgcca aagctcgcga agcagccttg gtcatcaggg tgcagtaatg
      961 taacattcat taccggatat tttccaccca gtgaggcgtc tttaattaaa atgccataac
     1021 ctgcagcgcg caaaccctta atgcccgccg caatacgcgg gtagcggtta attacgcttt
     1081 caggcacatc aggtaagcag aggccttcac ggataatctt gtatttaatg tcgcgctcaa
     1141 aaatttcggc caatgcctgc gtgcgggctt ccatgagtgt gttgccagca ctcatgccat
     1201 tgctcacata taagttgcca attaaattca ctggaaataa cacagtttga tcgtcacgca
     1261 gccgtttgta agggattgcg caaatgccac gcgctgaatt gcctgagttt aaatcaatca
     1321 gttggctggc aaccgttgtg ccttcggggt tataaaattg ctgcaactct ggtgttagta
     1381 atgctgtagg ccaacggtcg ccatctagtt ttatccattg ttcgtttgga taatgcacaa
     1441 aatcattgtt agctacattt ttacctaagt aaaaatgcgt ccaaaagtaa ttggtgctta
     1501 aacgctcaaa atattcgccc aatgcgcttg cgcgtgcggc caattgtgat gcgcctttac
     1561 cgttggtaaa aatgcgcggg caatccctgt caattacatg cacagaccaa atgccttcaa
     1621 tctcatttaa ccaagacttt tcttctacct caatgccaag cgccttaagt ttgccctgca
     1681 aagtaagaat tgaagactcg agcgaacggt ctttgctggg gataaaagtt tcgtgtgggt
     1741 tagaatcatt tggggtgttg tgcgtggtca tcgtgaattt tttttagaag taaaggctga
     1801 cagaatatca cgtcatgatt gatatgccgt ttttagtcat tgagaattta gtagatactg
     1861 gtcaactaat ttactaacct tgcaaaaggt atacacatct gtatactttc atacacttaa
     1921 aattttcctt tattgaaagt tcagcatgtc aactcatcac attcaatcaa tgcgcaatca
     1981 gttttataaa gctttcatgg tgttaacgtt gagtttgtct attaccgcgt gtgtgagcgt
     2041 atcaaatcaa caaaatgtag ccaaagaaca agattggcca agctacggac gtgattacac
     2101 caaccaacgc ttttcgccgc taacgcaaat taacccgcaa aatgtgaaaa atctaaccct
     2161 tgcttggcat ttcaaaagtg gcgtttctgc gagctttcag gcaaccccaa ttgtggttgg
     2221 aggaatcatg tatatttcac tcccctataa ccatgtggtt gcactagatg caaaaactgg
     2281 tcaagaacgc tggcgttacc aacatgagcg cagagcgcag tggaaaatgt gctgcggccc
     2341 agcgaatcgc ggtgttgctg tcaataatgg taaagtctac attggtactg tggacgctag
     2401 actcatcgcg ctagatgcca aaaatggcca aaaactttgg gatatcgatg tagcggatga
     2461 tacggcactt accgaaaatg ccagttcatt gagccattca gatgcaaaaa gcaaaaaaga
     2521 tatttatggt ggcacgggca ttggcattaa catggcgcca gttgtatata aaggcaaagt
     2581 gattattggc gtgacaggcg ttggctatgg cctgcactta gatacaccta cgcaagatgc
     2641 cccgctaggt gcagtagtag gcgtagatgg ccgatatggt cgcccaggct ttttagccgc
     2701 ttacgatgcg cttacaggta agcgcatctg gcaatttgaa acaattcctt cacaaggatg
     2761 ggaaggtgag tttgctgaaa caacgtcaga tggcatttcg cttaaccgcg acattgcaag
     2821 tgaaaaagcc aatttgcaga ataatcaaga tgcatggcgt tacggtggcg gttcagcatg
     2881 gagtacgccg gcaattgaca cacaaaccaa tacattgttt tttggcacag gtaatccatc
     2941 tccgcagatg aatgacatct ctaggccggg tgataatctt tacaccgttt cgcttgtggc
     3001 acttgatact gaaacaggta agcttaaatg gtattaccag caagttccgc atgatgtttg
     3061 gggttatgac ttagccagcc cgccagtatt gtttaattat c
//
LOCUS       DAOQSI010000202         3008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_37785_length_3008_cov_5.178463, whole genome shotgun sequence.
ACCESSION   DAOQSI010000202 DAOQSI010000000
VERSION     DAOQSI010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37785_length_3008_cov_5.178463"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /gene="obgE"
                     /locus_tag="PKL42_09815"
                     /gene_synonym="cgtA"
                     /gene_synonym="obg"
                     /gene_synonym="yhbZ"
     CDS             complement(<1..264)
                     /gene="obgE"
                     /locus_tag="PKL42_09815"
                     /gene_synonym="cgtA"
                     /gene_synonym="obg"
                     /gene_synonym="yhbZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167786.1"
                     /note="ObgE; essential GTPase; exhibits high exchange rate
                     for GTP/GDP; associates with 50S ribosomal subunit;
                     involved in regulation of chromosomal replication; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNU67410.1"
                     /translation="MKFIDEATIKIYAGDGGNGVATFRREKYEPMGGPSGGDGGRGGS
                     IILEADRNINTLVDYRYTRSFRAQRGENGRSAECYGAKGEDMVL"
     gene            complement(339..596)
                     /gene="rpmA"
                     /locus_tag="PKL42_09820"
     CDS             complement(339..596)
                     /gene="rpmA"
                     /locus_tag="PKL42_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNU67411.1"
                     /translation="MAHKKAGGSSRNGRDSHSQRLGVKAFGEQLVSAGSIIVRQRGTK
                     MHAGENVGMGKDHTLYAKADGKVSFAIKGALKRHTVSIIPA"
     gene            complement(616..930)
                     /gene="rplU"
                     /locus_tag="PKL42_09825"
     CDS             complement(616..930)
                     /gene="rplU"
                     /locus_tag="PKL42_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNU67412.1"
                     /translation="MYAVIKTGGKQYRVVAGERLKVEKLEVEVGGTVTLDQILMVSDG
                     DKTTIGSPIIKGATVKATVLSHGRGDKVMIFKFRRRKHYRKTQGHRQSFTEIKIEAIA
                     AK"
     gene            1205..2134
                     /locus_tag="PKL42_09830"
     CDS             1205..2134
                     /locus_tag="PKL42_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNU67413.1"
                     /translation="MLSVNAVIEKSLYSEVTLVNQVANYIINSGGKRLRPMLVLLSAG
                     LFGPIKPEHHQLAAVVEFIHTATLLHDDVVDESAKRRGQNTANALFGNAASVLVGDFV
                     YSRAFQMMVAVQNMRVMEVLSNATNVIAEGEVLQLLNVHNADISDDAYLKVIHYKTAK
                     LFEAATRLGAIIGNATADEEEALSQYGMHIGTAFQLIDDVLDLSGNVLEIGKNLGDDL
                     NEGKPTLPLLFAMRHGTPAESHAIKHAITHGGLQDFSTILSAVERTGALQHVRLVAEK
                     EAALALVAIQHIADSPYKIALQNLAEFAVKRNF"
     gene            2196..>3008
                     /locus_tag="PKL42_09835"
     CDS             2196..>3008
                     /locus_tag="PKL42_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67414.1"
                     /translation="MIKKWCFFLVLLCGMQTNTFAQPAEPAKDTVKVGIYITNLYDFD
                     LADGSFVAEFWTWSLHNNDNLSFKDTQEVIKAKKTICSNFATQKKANLNWEQKKCVAT
                     ILQDWDIRNFPFDTQELVINLEETMLDSAAVAYVADTENSKINHNLSLDEWHINAFNI
                     QAKNAEYDTTYGDPELQGSSSYPSVQATVSLSRTSSWTTFIKLVTGLYVAFLIALMVF
                     RIKPPDSDNRVELAVGGLFAAVGNKYIVESIVPTSTQNTMIDNMHNITFGAIL"
BASE COUNT          840 a          707 c          637 g          824 t
ORIGIN      
        1 caacaccatg tcttcacctt tggcaccata acactcggca ctgcgaccat tttcaccacg
       61 ctgcgctctg aatgatcgag tatagcgata atccactaaa gtattaatgt tgcggtctgc
      121 ttcaagaatg atagaaccac cgcgaccacc atcaccgcca ctggggccgc ccattggctc
      181 gtatttttca cgacgaaacg tggcgacgcc attaccgcca tcgcccgcgt agattttaat
      241 ggttgcttcg tctataaatt tcataattgt gtgagtttac caaataccaa ataaaaaagc
      301 cccatcaaac gatgaggctt tgaaatttgc aatgtaactt aagcaggaat aatgcttaca
      361 gtgtggcgtt ttaaagcgcc tttaattgca aaagaaactt taccgtcagc ttttgcgtat
      421 agagtatgat ctttacccat gccaacattt tcaccagcat gcatttttgt accgcgctga
      481 cgcacgataa tgctaccagc tgaaaccaat tgctcaccaa aggcttttac gcccaaacgt
      541 tgtgagtgtg aatcgcgacc gttacgtgaa ctaccgcctg ctttcttgtg tgccatgatt
      601 taatccttta attacttatt tagcagcaat tgcttcaatt ttgatttcag taaaactttg
      661 gcgatggcct tgtgttttac ggtaatgttt acggcgacgg aatttaaaaa tcatcacttt
      721 gtcaccacgg ccatgtgaaa gcactgtggc ttttacagtt gcgcctttaa taattggtga
      781 accaatagtc gttttatcgc catcagatac cattaaaatt tgatcaagcg tgacagtgcc
      841 gcccacttca acttcaagtt tttcaacttt taatctttcg cctgcgacga cgcgatattg
      901 cttaccacct gttttaatta ctgcatacat gattgagcct taaattacgc tttctaaaga
      961 actcggcatt atacgcaaat cgcgatgcat tgcaaaccaa attcgcaaca caccctaaaa
     1021 aaatatcacg cccgcctaag tagggtgatt atctcatgat ttgtcgggct tcaactcaat
     1081 acacctgaat aggctgtgat aaaatagcgg caaaatttat aggtgcattc tcgtgatatc
     1141 cagtgtcatt tcgtccaacc gtaccaacca gctcaacggc gtgcaagact gcattgcaaa
     1201 agatatgttg agcgtcaatg ctgtgattga gaaatcactc tactctgaag tcacgctcgt
     1261 caatcaggtg gctaattaca ttatcaatag cggtggcaag cgcttgcgcc caatgctggt
     1321 gcttttgtct gccggcttgt ttggccccat taagcctgag caccatcaat tggctgccgt
     1381 ggttgagttt attcacaccg ccaccttgct gcatgatgac gtggtggatg aatcagcaaa
     1441 acggcgtggc caaaacacgg ccaacgcact ttttggcaat gcggccagtg tattggtggg
     1501 tgactttgtc tactctcgtg cttttcagat gatggttgcc gtacaaaata tgcgcgtgat
     1561 ggaagtatta agtaacgcca ccaacgtgat tgccgagggc gaagtgctgc aattattgaa
     1621 tgtgcacaat gctgacattt cagatgatgc ttatctaaaa gtgattcact acaaaaccgc
     1681 taaattattt gaagccgcaa cgcgtcttgg cgcgattatt gggaacgcca cggccgatga
     1741 agaagaagcg ctcagtcaat acggtatgca tatcggcacc gcctttcagt tgattgatga
     1801 cgtgctcgat ttaagtggta acgtgttgga aattggcaaa aatctggggg atgacctgaa
     1861 cgaaggcaaa cccactttgc cattactgtt tgccatgcgc cacggcacac cggctgagtc
     1921 acacgccatc aaacacgcca ttacgcatgg cggcctgcaa gatttctcta ccatccttag
     1981 tgctgttgag cgcacgggtg ctttgcagca tgtacgctta gtggctgaaa aagaagccgc
     2041 acttgcgctt gtggccattc aacatattgc cgattcacct tacaaaattg cattacaaaa
     2101 cttggcggag tttgcggtaa agcgcaattt ttaataaaat tttacaaatt atcgccacat
     2161 gtacacacta taactcatgg tgattggggg cgtacatgat taaaaagtgg tgttttttct
     2221 tggtgttgtt gtgcggcatg cagaccaaca cttttgcaca accagccgaa cctgccaaag
     2281 acaccgttaa agtcggcatt tatattacca acctctacga ttttgattta gccgacggta
     2341 gttttgtggc cgaattctgg acatggagcc tgcacaacaa cgataacttg agctttaaag
     2401 acacccaaga agtcattaaa gctaaaaaaa caatttgctc taattttgcc acgcaaaaaa
     2461 aagccaatct caattgggag caaaaaaaat gcgtcgccac cattttgcaa gattgggaca
     2521 ttcgaaattt tccgtttgac acgcaagaac tggtcatcaa cttagaagaa accatgcttg
     2581 actccgctgc ggtggcctat gtagcggata ccgaaaattc caaaatcaac cacaacttat
     2641 cgcttgatga atggcacatc aacgctttca atattcaggc caaaaatgcc gaatacgaca
     2701 ccacgtatgg tgacccggaa ttgcaaggca gcagcagtta ccctagcgta caagcaactg
     2761 tgagcctttc aagaacatct tcctggacga cctttattaa gctcgtcacc ggcttatatg
     2821 tagctttttt aattgcgctt atggtgttta gaattaagcc gcccgattct gataaccgcg
     2881 ttgaacttgc agtgggcggg ctatttgcag cggtgggtaa taaatacatt gtagaaagta
     2941 ttgtgccgac atccacacaa aacaccatga tcgacaatat gcacaacatc acgtttgggg
     3001 cgatttta
//
LOCUS       DAOQSI010000203         2954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_38834_length_2954_cov_5.554329, whole genome shotgun sequence.
ACCESSION   DAOQSI010000203 DAOQSI010000000
VERSION     DAOQSI010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38834_length_2954_cov_5.554329"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="PKL42_09840"
     CDS             complement(<1..512)
                     /locus_tag="PKL42_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNU67415.1"
                     /translation="MCGIIGIVGKNPVNQLLYDGLLVLQHRGQDAAGIVTTDGNTFYM
                     HKNNGLVKDVFQTRHMRSLIGNVGIGHVRYPTAGSSSAAEAQPFYVNSPFGIVLGHNG
                     NLTNSEQLKSEMFRQDLRHINTNSDSEVLLNVLAHEIEQTSRNAVLNTDMTFDAVAGV
                     HRRCRGAYAVV"
     gene            complement(612..1103)
                     /locus_tag="PKL42_09845"
     CDS             complement(612..1103)
                     /locus_tag="PKL42_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNU67416.1"
                     /translation="MTIFDYIVLAIVGVSIVISVMRGLLAEVLSIAGWFAAFFVAKTY
                     ANQLVPMMPADIPTESLRILAAFVILFLATFLLTTLLAIALTTIFKKIGLGWLNRILG
                     AVFGLARGMLIVCIVVFLAGLTNVPKDERWKNAMFSAPIEALVISLLPWVPASIASHI
                     KYE"
     gene            complement(1141..1878)
                     /locus_tag="PKL42_09850"
     CDS             complement(1141..1878)
                     /locus_tag="PKL42_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNU67417.1"
                     /translation="MPPEISNDQELSLKKRARRRLVGAIALVILMIIILPMILEDRTA
                     VTPDTPIKITMPDEAALDAESNQHVQLNDVVESAPAETPNTNAQQFADVQPSNANSHT
                     EPTPVATAEIVPAVGHDEVVKADEVKKEEKPAVKKPEVQKVEEKKPLEKPAETKVGSV
                     DAKSVTQSSGSFTIQVGVYSDAANVKQLQAKLKEAGYNSRTEKISTPKGEKIRLRVGK
                     FASRQDAADALIKINAAGLSGMVMSND"
     gene            complement(1982..>2954)
                     /locus_tag="PKL42_09855"
     CDS             complement(1982..>2954)
                     /locus_tag="PKL42_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNU67418.1"
                     /translation="TYFEVGTLAAMWHFMHAVEHKLDVVILEIGLGGRLDAVNAFVPD
                     CAIVTSVDLDHQEFLGDTREAIGFEKAGVFRALKPAICGDTSPPNSLVQYAKKIHANF
                     NTIHQDFGIKLTENGWQYTAHGTVVYSLPLPALQGDYQLINAACAVAAVDSLQISLSV
                     SEAAIGNGLRKVKVAGRFQILHQHDLGKISTTLIFDVAHNPHAAKALAQNLNGYKNSG
                     KTFAVFAMLADKDIRGVVEAVMHEIDAWYVATIDHVRGEKAENLAGLIAAIQPQAQVK
                     TFHDAYIAYQHARIDLEACNDVNENDKIVVFGSFFTVSTVMQHLALN"
BASE COUNT          823 a          736 c          561 g          834 t
ORIGIN      
        1 actaccgcat aagcacctct gcaacgccga tgcacgcctg cgaccgcatc aaacgtcata
       61 tcggtattca atactgcatt acgtgaagtt tgttcaattt cgtgtgcaag tacgttcagt
      121 aaaacttcag aatcagaatt agtgttgata tggcgtaaat cttggcggaa catctcagac
      181 tttaattgct cagaatttgt gagattaccg ttgtggccca acacaatccc aaacggggaa
      241 ttcacataaa aaggttgcgc ttctgccgca ctggatgaac ccgccgttgg gtagcgcaca
      301 tggccaatgc caacattgcc aattaaactg cgcatatggc gcgtttgaaa gacatctttt
      361 accaagccat tgtttttatg catataaaaa gtattgccat ccgtggtgac aatacctgcg
      421 gcatcctgcc cgcggtgttg caacaccagc aagccatcat acaacagttg attcacagga
      481 tttttaccta cgatgccaat aattccgcac atgaatatcg ccttcagtga ttaatttcaa
      541 aaaataaaaa tttcgtaagc tcttatttgc aagttcttat tcgggtgttc ttagaataat
      601 aaattgcgca tttactcgta cttgatgtgg ctcgcaatgc tagcgggaac ccatggcaac
      661 aggctgatga ccagcgcttc aatgggcgca ctaaacattg catttttcca gcgctcgtcc
      721 ttgggaacat tcgtgagccc agctaaaaat acgacaatac acacgattaa catcccacgc
      781 gccaaaccaa acacagcgcc caatatgcgg tttaaccaac ctagcccaat ttttttaaaa
      841 atcgtcgtca gcgcaatcgc aagcaaggtt gtgagcaaga atgtggctaa aaatagaatc
      901 acaaatgccg ccaatatgcg taacgattcc gttggaatgt ctgccggcat catagggacc
      961 aattgatttg cataagtttt tgccacaaaa aaagccgcaa accaacccgc tatcgacagc
     1021 acttctgcca acaaaccacg catgacacta atgacgatgg aaacacccac aatcgcaagg
     1081 acaatgtagt caaatatcgt catgattgcc attaattaca acaattcatt gaaataaaac
     1141 tcaatcatta ctcattacca tgcctgataa accagcagca ttgattttga tgagggcgtc
     1201 agccgcatct tgtctggatg caaacttacc gactctcaaa cgaattttct caccctttgg
     1261 tgttgaaatc ttttcggtcc gtgaattata tccagcctct ttcaatttag cctgcaattg
     1321 cttcacattg gctgcatctg aatatacacc cacttgtatt gtgaaagaac cacttgattg
     1381 agtcacagat tttgcatcaa cactacctac tttagtttca gcaggtttct ccagtggttt
     1441 tttttcttca accttttgta cttccggttt ttttacggct ggcttttctt ctttcttaac
     1501 ttcatccgcc tttactactt cgtcatgccc tacagcaggc actatttctg ccgtggctac
     1561 aggcgttggc tccgtatgcg aattggcgtt tgacggctgc acatctgcaa attgctgcgc
     1621 attggtgttc ggcgtttctg cgggagcact ttcaaccaca tcatttaact gaacatgttg
     1681 gttactttca gcatcaagcg ccgcctcgtc aggcatggta atcttaatag gcgtgtctgg
     1741 cgtcactgcg gtgcgatctt ccagaatcat tggcaaaata ataatcatca agataactaa
     1801 agcgatcgca cctaccaaac gacgtctggc acgttttttt aaacttaatt cttgatcatt
     1861 cgagatttca ggtggcattg ctgctttcct caaaggttaa ttatttgaat gccgctttag
     1921 catttttggc ttgccatctt gcgcaaaaac tagctcaact gattgatgcc aaacgctcaa
     1981 cttaatttag tgccagatgc tgcatcaccg tcgaaacagt gaaaaatgaa ccaaacacga
     2041 ctattttatc attttcattc acatcattac aggcttctaa atcaatacgc gcatgctgat
     2101 aagcaatata cgcatcatga aaagtcttaa cctgcgcttg gggctgtata gccgcaatca
     2161 atccagccaa attttccgct ttttcaccgc gcacatggtc aatggttgcc acataccatg
     2221 catcaatttc atgcatcaca gcttctacca cgccgcgaat atctttatct gcaagcatgg
     2281 caaaaactgc aaaagtttta cctgagtttt tatacccatt taagttttgt gccagcgctt
     2341 tggccgcgtg tggattatgc gccacatcaa atatcaatgt cgtacttatt ttacctaggt
     2401 catgctggtg caaaatttga aagcgccctg ccacttttac tttacgcagc ccgttaccaa
     2461 tagcagcctc tgatactgac aaagagattt gtaacgaatc caccgcagct accgcacagg
     2521 ccgcattgat gagctggtag tcaccttgca aagctggcag tggcaacgaa taaacaactg
     2581 taccgtgcgc agtatattgc caaccatttt cagtcagctt aatcccaaag tcttgatgaa
     2641 ttgtattgaa attggcatga atttttttgg cgtactgcac taggctgttt ggtgggcttg
     2701 tgtcgccgca tattgcgggc ttcaatgcac gaaacacacc agctttttca aaaccgattg
     2761 cttctcgtgt gtcaccaaga aattcttgat gatccaaatc cacactcgtc acgatggcac
     2821 aatcgggtac aaaagcattg accgcatcta gcctgccacc taaaccaatt tctaaaatca
     2881 ccacatctaa tttgtgctct accgcgtgca tgaaatgcca catcgcagcc aaagtcccca
     2941 cttcaaaata ggta
//
LOCUS       DAOQSI010000204         2906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_39855_length_2906_cov_4.440547, whole genome shotgun sequence.
ACCESSION   DAOQSI010000204 DAOQSI010000000
VERSION     DAOQSI010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39855_length_2906_cov_4.440547"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1863)
                     /locus_tag="PKL42_09860"
     CDS             complement(<1..1863)
                     /locus_tag="PKL42_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNU67419.1"
                     /translation="MTTHHQEEISRPVVLSRKLGISGRIAQYFLSAQITPLIALVSLL
                     LGIFAVLVTPREEEPQINVTMANVLIPFPGASAKNVEQMVSIPAEQVLSQIADIEHVT
                     SVSRPGMSVITVQFQVGVPRTEALVRLYNTIQSNQDWLPKNLGVGEPIVKPKGIDDVP
                     ILTATLYTKNNQKGAYELERVAHALEAEIKHIKGTREVVTLGGPGRAVNIDVDPVRMA
                     AANISLADMRRALQSANLGAPLGEVTNNNQSIALEVGKYFTDAEDVAQLVVGLHNGHP
                     VYLGEVAEIHDGPPPAERYVWYGEAGKHVAEYPAVTMSVTKKPGQNAVAVADAVIKQI
                     DSLKNVLIPADIDVKITRNYGETANDKAQKLIQKLIFATSAVIALVFIALGRREAAIV
                     GVAVILTLTATLFASWAWGFTLNRVSLFALIFSIGILVDDAIVVVENIHRHMKLKPAA
                     SLTEIIPMAVDEVGGPTILATLAVIAALLPMAFVTGLMGPYMSPIPINASMGMLISLA
                     VAFIITPWLSRIWLRLAHHEDSLTKKIAPAFTRLFNPFLSETKGKNNRRNLWLVILGL
                     IILSLALPVAKLVVLKMLPFDNKSEFQVVVDMPAGTPPEKTAAVLHAMTAYLATV"
     gene            complement(1872..>2906)
                     /locus_tag="PKL42_09865"
     CDS             complement(1872..>2906)
                     /locus_tag="PKL42_09865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNU67420.1"
                     /translation="PFSTLTNAAGLATITIKQSNAKTQYIAEGVVEAVKSSVIAPQVS
                     GSITELPVKAGDYVKAGQLLVRIDTRMAAQQSAANQAQVVAAQAQVAAAQAQMSTARK
                     EFERKQRLYEKQYISQAALERAESDYKTAQAQTNTQIAQAKAQHAQSGLSNVQTGLHT
                     ITAPYSGVIAEVMTEVGGMAMPGQPLLTLYDPNGFRVSVNVPQSQLAQLKAGPNINVT
                     VPAASVVERSLISTNLTVLPTADSVSNMSTVRLTLPQNITSIRPGMFARAMLPVNDAR
                     GKDQIFVPVKSVIKRSELVAVYVVDKQGHPQLRQVRLGRKQGENIEVFAGLQPGESVA
                     LDPIAAANVK"
BASE COUNT          714 a          701 c          680 g          811 t
ORIGIN      
        1 cacggtggct agataagcgg tcatggcatg caatacggca gctgtttttt ctggtggggt
       61 gcctgcaggc atatccacca ccacttgaaa ctcggattta ttatcaaacg gcaacatctt
      121 gagtacgacc agtttggcca ctggtaatgc tagcgataga ataattagcc caagtatcac
      181 gagccataaa ttacgtcgat tatttttacc ctttgtttca cttaaaaatg ggttaaaaag
      241 tcgcgtaaaa gcaggggcaa tttttttagt aagtgaatct tcatggtgtg caaggcgcaa
      301 ccatatgcgc gataaccaag gcgtaataat aaaagccaca gccagtgaaa tgagcatgcc
      361 catgctggca ttgataggaa ttgggctcat gtaagggccc attaaacctg tcacaaatgc
      421 catcggcaat agcgcggcaa tcaccgctag tgtagcaagt atcgttgggc cgcctacctc
      481 atctactgcc attgggataa tttctgtcag gcttgccgct ggcttaagct tcatatgccg
      541 atgaatattc tcgaccacca cgatggcatc atcgaccaaa ataccaatcg agaaaattag
      601 cgcgaataat gaaacacggt taagcgtaaa cccccatgcc caagatgcaa atagcgtggc
      661 agtgagcgtg aggataactg ctacgccgac aattgccgcc tcgcgccgac ccagcgctat
      721 aaataccagt gcaattacgg cagaagttgc aaaaatcagt ttttgtataa gtttttgcgc
      781 tttatcattg gcggtttcac cgtagtttct ggtgattttt acgtcaatgt ccgcaggaat
      841 caacacattt ttcaggctat caatctgctt gatgacggcg tctgccactg ccacggcatt
      901 ttggccaggc tttttagtaa cactcatcgt tactgcagga tattccgcta cgtgcttgcc
      961 agcttcgcca taccaaacat aacgttctgc ggggggtggt ccatcatgaa tttctgccac
     1021 ttcacccaaa tatacgggat gtccgttatg caaccctacg actagttgtg caacatcttc
     1081 agcatcggta aagtatttac ccacttctag ggctattgat tgattgttat tggtgacctc
     1141 gcccagtggt gcgccaagat ttgccgattg caaggcgcgg cgcatgtcgg caagtgaaat
     1201 attagctgca gccattctca ctgggtccac atctatattt accgcgcggc ctggcccacc
     1261 aagtgttacc acctcgcgcg tgccttttat atgcttgatt tcagcttcta gtgcatgtgc
     1321 tacacgctca agctcatacg cgcctttctg attgtttttg gtatacaacg ttgccgttaa
     1381 aataggcaca tcatcaatac ctttgggctt aacgataggt tcacccacac caaggttttt
     1441 cggcaaccaa tcttgattgg attgaatggt gttgtaaagg cgaactaagg cttcagtacg
     1501 cggaacgcca acctgaaact gtacagtaat gactgacatc ccagggcgag aaaccgaagt
     1561 cacatgctcg atatctgcaa tttgtgaaag cacctgttca gctggtattg aaaccatttg
     1621 ctctacattt ttggctgatg cgccagggaa gggaatcagc acattcgcca tggtcacatt
     1681 gatttgtggc tcttcttcac gcggcgtcac cagcactgca aatatgccaa gcaacaatga
     1741 caccaaagca attaaaggcg taatttgagc ggataaaaaa tactgagcga ttcgcccaga
     1801 aatgcctaat ttacgactaa gcactacggg cctgctgatt tcttcttgat gatgcgtcgt
     1861 cacagatatt cctacttaac atttgcggct gcaattgggt caagtgcgac tgattcgcca
     1921 ggttgtaaac cagcaaagac ctcaatattt tcgccttgct tacggcccaa ccgtacttgc
     1981 cttagttgtg gatggccttg tttatctacc acatagactg caaccagttc actgcgttta
     2041 atcacagact ttacgggcac aaaaatttga tctttgcctc ttgcatcatt tacaggcagc
     2101 attgcccgag caaacatgcc tggcctgatg cttgtgatat tttgaggcaa ggtcagacgg
     2161 actgttgaca tgttgcttac tgaatctgcc gtaggtaaca ccgttaagtt ggtgctgatt
     2221 aaactacgtt ctaccacgct tgcggcaggc acagtgacat tgatatttgg cccagctttt
     2281 aattgagcca gctggctttg cggcacgttt acgctaacgc gaaatccatt tgggtcatat
     2341 agcgtaagta agggttggcc aggcatcgcc atgccgccca cttccgtcat tacttctgcg
     2401 attacgccag aatatggcgc agtgatggta tgcaggcccg tttgcacatt agataaccct
     2461 gattgcgcat gctgtgcttt agcttgtgca atttgtgtgt tggtttgcgc ttgagcggtt
     2521 ttgtaatctg attctgcacg ttctagcgcg gcctggctga tatattgttt ttcatataac
     2581 cgttgcttgc gctcaaattc tttgcgggcg gtactcatct gtgcttgtgc agcggccact
     2641 tgtgcttgag ctgcaacgac ttgcgcctga tttgccgctg attgttgagc tgccatgcgt
     2701 gtatcgatgc gcacaagtaa ctgaccagct ttgacgtaat cgccagcctt gacggggagt
     2761 tcggtaatgc tgccagatac ttgtggcgca atgacactgc tttttaccgc ttccacaacg
     2821 ccttcagcga tgtattgcgt tttagcattt gattgtttaa tggtaatcgt ggccaagcca
     2881 gccgcattcg tcaatgtgga gaaggg
//
LOCUS       DAOQSI010000205         2894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_40150_length_2894_cov_3.348010, whole genome shotgun sequence.
ACCESSION   DAOQSI010000205 DAOQSI010000000
VERSION     DAOQSI010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40150_length_2894_cov_3.348010"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PKL42_09870"
     CDS             <1..493
                     /locus_tag="PKL42_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNU67421.1"
                     /translation="FDGLPTLSSTLSLLMAEAEQHHCGRQAAIDRLFEYLLIQLLRHI
                     LDHQQINIGLLAGLADKRLAKAITAMHNAPSRAWSLESLAQEAGMSRARFAVRFREIV
                     GTTPIENLTQWRIGLAKNLLKQGKPIGLVANEMGYSNAAVFTRAFKAAVGQTPKTWTG
                     IQT"
     gene            complement(653..1447)
                     /locus_tag="PKL42_09875"
     CDS             complement(653..1447)
                     /locus_tag="PKL42_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNU67422.1"
                     /translation="MDTRRKELAEFLTALRQRGTPEEFGFPTGARRRTKGLRREEVAQ
                     LAGISATWYTWIEQSREVNVSAEALERLATALKLSKSERTYLFDMADRRDPQAHLLEA
                     DAAPDTLVNMLGKILIPAYIMGRTWDLLAWNKPAEELFNGWLDVWNKDMLQPNLLRFV
                     FLHANAKLFVVNWELRARRLVAEFRADCRSRLEEPALQKLVNELTQANPEFDRFWKQH
                     DVLERQGGQREFNHTKHGLISYQQVTLRPVEQEQLKLVLLQPEKLI"
     gene            1596..>2894
                     /gene="leuC"
                     /locus_tag="PKL42_09880"
     CDS             1596..>2894
                     /gene="leuC"
                     /locus_tag="PKL42_09880"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNU67423.1"
                     /translation="MAGKTLYDKLWDSHVVQEDADGTCLIYIDRHLVHEVTSPQAFEG
                     LRLAGRKPWRADTIVANPDHNTPTKDWAKGIEDPISRLQVETLDSNIKETGALVYFPF
                     KHANQGIIHVIGPENGTTLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTL
                     IAKKSKRMLVRVDGELGVGVTAKDVALAVIGKIGTAGGNGFAIEFGGDVIRALSMEGR
                     MTICNMAIEAGARAGIIAPDAKTAEYLKGRQYAPKPADWDKAVVYWNTLASDADATFD
                     SVVVLNGADIAPQVTWGTSPEQVLPINGLVPDPAQEKDATKRTSIENALKYMGLTANT
                     PIEQIKLDKIFIGSCTNSRIEDLRAAAVVAKGKKVASSIALAMVVPGSGQVKRMAEAE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLSPGERCASTSNR"
BASE COUNT          764 a          622 c          703 g          805 t
ORIGIN      
        1 gtttgacgga ttacccacat tgagtagcac cttaagtttg ttaatggctg aggccgagca
       61 acatcattgt ggtcgccaag cggcaattga tagattattt gaatatttgc tgattcaact
      121 tttgcgtcac attttagacc atcaacaaat caacattggc ttgcttgctg gtctagcgga
      181 taaacgatta gctaaagcta ttaccgctat gcacaatgcg cccagccgtg catggtcatt
      241 agaatcttta gcacaagaag ctggcatgtc gcgcgcacgt tttgccgtgc gttttagaga
      301 aatagtcggt acaacgccta tcgaaaactt aacgcaatgg cgcataggtc tagctaaaaa
      361 cttacttaaa caaggcaaac ccatcggttt ggttgccaat gaaatgggtt atagcaacgc
      421 tgctgtgttt acgcgcgctt ttaaggcggc ggttgggcaa actccaaaaa cttggactgg
      481 catacaaact tgagttgcgt attaatgcgt agcgtgtgca tactgcttat ttataggcac
      541 actgttcatc acaattattc cccatactct catttttcat catcatagtt accacaaatt
      601 tctgcttaca tccactcaga ggcatgaaat tctcttggta attttttaag cactaaatta
      661 atttttctgg ttgcagtaaa actagcttaa gttgttcttg ttctacaggt cgcaacgtta
      721 cttgttggta gctgattaag ccgtgtttcg tatggttaaa ttcgcgctga ccgccttgcc
      781 tttctagcac atcgtgttgt ttccaaaagc gatcaaattc tgggttggct tgcgtgagct
      841 cgttgacgag tttttgcaaa gcgggttctt ctaaacggct gcggcaatcg gcgcgaaact
      901 ctgctactag cctgcgtgcg cgtagctccc agttcaccac aaatagtttg gcgtttgcgt
      961 gtaaaaatac aaagcgtagc aaattgggtt ggagcatatc tttgttccaa acatccagcc
     1021 agccgttaaa taattcctcg gcgggtttgt tccacgccag taaatcccag gtacgcccca
     1081 taatataggc gggtattagt attttaccca gcatatttac aagtgtatca ggtgctgcgt
     1141 ccgcttctag caaatgggct tgtgggtcgc gtctgtctgc catgtcaaat aaataggtgc
     1201 gttctgattt gctgagttta agtgcggtgg ctagtcgctc taaagcttca gcagacacat
     1261 taacctcacg gctttgttct atccatgtat accaagtggc gctgatgcca gctagttgcg
     1321 ctacttcttc acggcgtagt ccttttgtac gcctgcgtgc gcctgttggg aaaccaaact
     1381 cttcaggtgt gccacgttgg cgtagcgcag tcaaaaattc agcaagttct tttcgtctcg
     1441 tatccattta atttcacttt ttctattcga tttctattca atgtattgct agcctatcat
     1501 atttaatact agtataaact atattcttgt acaggtattg gtgatgattt agaatgctgt
     1561 aagttttaaa aaattacctt tttaaggagt taaacatggc gggaaaaacg ctttacgata
     1621 aattatggga ttcgcacgta gtgcaggaag atgcagacgg cacttgttta atttatattg
     1681 acagacattt ggtgcatgaa gtgaccagcc cgcaagcgtt tgaaggcttg cgcttggctg
     1741 gccgtaaacc atggcgtgcc gacactattg tggcgaaccc tgaccacaac acgccaacca
     1801 aagattgggc gaaaggcatt gaagacccaa tttcacgttt gcaagtagaa actcttgata
     1861 gcaatattaa agaaactggt gcgttggtgt actttccgtt caagcacgcg aatcaaggca
     1921 ttattcacgt gattgggcct gaaaacggca caactttacc cggcatgacg gtagtgtgtg
     1981 gtgatagcca tacttctacg cacggtgcgt ttggcgcatt ggcacacggc attggtactt
     2041 ctgaagtaga gcacgtatta gcgacacaaa ccttaatcgc aaaaaaatca aaacgcatgt
     2101 tagtgcgtgt agatggcgaa ttgggcgtgg gtgttaccgc aaaagacgtg gctttggcgg
     2161 tgattggtaa aattggtaca gcgggcggca atggttttgc cattgagttt ggcggcgatg
     2221 taattcgtgc cttaagcatg gaaggccgca tgaccatttg caatatggcg attgaagcag
     2281 gcgcacgcgc tggcattatc gcgccagatg ccaaaacagc agaatactta aaaggccgcc
     2341 aatacgctcc aaaaccagcg gattgggata aagcagtggt ttactggaat acattagcga
     2401 gcgatgccga tgccacgttt gacagcgtgg ttgtgctgaa tggcgctgat attgccccgc
     2461 aagtcacttg gggtacatcg ccagagcaag tgttgccgat taatgggttg gtgccagacc
     2521 ctgcacaaga gaaagacgca actaagcgca ccagtattga aaatgcactg aaatacatgg
     2581 ggttaaccgc gaatacgccg attgaacaaa ttaaactcga taaaatcttt attggctcat
     2641 gcactaacag ccgtattgaa gatttacgcg ccgcagcagt cgttgctaaa ggtaaaaaag
     2701 tggcaagcag tattgcatta gccatggtgg tgccaggttc tggccaagta aaacgtatgg
     2761 ccgaagcgga aggcttagac aaaattttta ttgaagcagg gtttgaatgg cgcgagccag
     2821 gttgcagtat gtgtttagcc atgaacgcgg atagactcag cccaggtgag cgttgtgctt
     2881 caaccagtaa ccgt
//
LOCUS       DAOQSI010000206         2798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_42307_length_2798_cov_4.398104, whole genome shotgun sequence.
ACCESSION   DAOQSI010000206 DAOQSI010000000
VERSION     DAOQSI010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42307_length_2798_cov_4.398104"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..351)
                     /locus_tag="PKL42_09885"
     CDS             complement(76..351)
                     /locus_tag="PKL42_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNU67424.1"
                     /translation="MANKSELIDQIAEAAGISKAAAGRALDATTASITTALKKGELVT
                     LIGFGTFYVGKREARSGRNPRTGATINIKAANSPKFRAGKSLKDAVN"
     gene            complement(480..>2798)
                     /gene="lon"
                     /locus_tag="PKL42_09890"
     CDS             complement(480..>2798)
                     /gene="lon"
                     /locus_tag="PKL42_09890"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNU67425.1"
                     /translation="SEGDKQILLVAQKSANKDEPSAEDLYEVGTVATVLQMLKLPDGT
                     VKVLVEGVQRAKFSGFTETEECFAAHVELIAESVDDIEIQALMRTVFAQFDQYVKLNK
                     KIPPEILTSLATIDDAGRLADTIAAHLTLKLDEKQKVLEMFSVAERLEHLLRLMEGEI
                     DILQVEKRIRGRVKRQMEKTQREYYLNEQVKAIQKELGEQDENAEMDELEKRIQDAHM
                     PKEALAKVASELKKLKMMSPMSAEASVVRNYIDTLINLPWKKKTKISKDLLAAEKILD
                     DDHYGLEKVKERIVEYLAVQQRVDKIKAPILCLVGPPGVGKTSLGQSIAKAVNRKFVR
                     MALGGVRDESEIRGHRRTYIGSMPGKILQSMSKVGVKNPLFLLDEVDKMGQDMRGDPS
                     SALLEVLDPEQNHTFADHYVEVEYDLSDVMFVATANSMNIPAALLDRMEIIHLSGYTE
                     DEKVNIAMRYLVPKQLKSHGLKDAEVTVSDVAVRDIVRYYTREAGVRKLEQEISKVCR
                     KVVKELLTAKAKEGVKATKKIIVTPKVLDKYLGVQRYDYGVAAKENQIGQVTGLAWTS
                     VGGELLTIEAVLLPGKGKTTTTGKLGEVMKESTLAAMSVVRSRAKRLGIADDFYEKND
                     IHIHFPEGATPKDGPSAGIAITTALVSILTGIPVRADVAMTGEITLRGEVLPIGGLKE
                     KLLAAHRGGIKTVLIPLQNVKDLADIPENIKNKLDIHPVKWIDEVLALALEKLPSPLP
                     ENELVSDLSAKEVQPADKTLESNVVISDNTKH"
BASE COUNT          740 a          696 c          497 g          865 t
ORIGIN      
        1 aaccgctgac attcgccttg taagggcgac gctctaccaa ctgagctaag caccctgctt
       61 aatcaacaaa gaatgctagt ttacagcatc tttaagagat ttaccagccc taaatttagg
      121 agaatttgca gctttaatat taattgttgc accagtacgt gggttacggc cactacgtgc
      181 ttcacgctta cctacataaa atgtaccaaa gccaattaaa gtgactaatt cgcccttttt
      241 caatgctgta gtaattgaag ctgtcgttgc atctaatgca cgaccagctg ccgcttttga
      301 aatacctgct gcttctgcaa tttggtcaat taattctgat ttatttgcca cttgtactct
      361 cccttgttta agatggattt aattcactca ttctgagcaa ctaatactca tacagcttta
      421 tatcaagcct acaaagccca tgtcaagctt ttatcagcat gttcgtgcat ttttttaaat
      481 taatgcttcg tgttgtctga aattacgaca ttactctcaa gcgttttgtc agcaggctgc
      541 acttcttttg ctgaaagatc actgaccaat tcattttctg gtaatggaga aggaagtttc
      601 tccaatgcca acgccaaaac ttcatcaatc cattttacag gatgaatatc cagcttgttt
      661 tttatgtttt caggaatatc ggcaaggtct ttaacattct gcaatggaat caaaactgtt
      721 ttgatgccac cacggtgtgc cgcgagcaat ttttccttca agccgccaat cggcaagacc
      781 tcgcccctta aggtaatttc gcctgtcatt gcaacatccg cacgcactgg aatcccagtg
      841 agaatagaga ctagcgcagt agtaatcgca atccctgcac tgggaccatc ttttggcgtc
      901 gcaccttcag gaaaatgaat gtgaatatca tttttttcgt aaaagtcatc tgcaatgcca
      961 agccgtttgg cccggctacg caccacgctc attgcagcca gtgtagactc cttcatcact
     1021 tcaccaagct tacctgtggt cgttgtttta cctttgccag gcaacaacac ggcttctatc
     1081 gttagcaact cacccccaac agaagtccac gccaaccctg tcacttgacc tatttgattt
     1141 tctttcgcgg ccacgccata gtcgtaacgc tggacaccca aatacttatc taggactttt
     1201 ggcgtgacta tgattttttt agttgctttt actccttctt tagccttagc ggtcagtaat
     1261 tccttcacaa cttttctaca gacttttgaa atttcttgtt ctaatttacg aacaccggct
     1321 tctctagtgt agtaacgcac aatgtcacga accgctacat ctgaaacagt gacttcagcg
     1381 tcctttaagc catgtgattt caattgctta ggcactaagt aacgcatcgc aatattcact
     1441 ttttcatctt ccgtatagcc tgagagatga atgatctcca tacggtctaa cagtgcagca
     1501 ggaatattca tcgagtttgc ggtagcgaca aacatcacat cagataggtc atattcaact
     1561 tctacataat gatccgcaaa agtatgattt tgctcaggat caagcacctc caacaaggct
     1621 gaagaagggt caccgcgcat atcttgaccc atcttatcga cttcatcaag taagaataaa
     1681 gggtttttaa caccgacttt gctcatactc tgcaaaatct tacctggcat tgaaccgata
     1741 taagttcttc tgtgacctcg aatttcagat tcatcacgca caccacccag cgccatacgt
     1801 acaaacttgc gattaacggc ttttgcaatg ctctgaccca atgaggtttt accaacgcca
     1861 ggtggaccaa ccaagcaaag aataggcgcc ttaattttat ctacacgctg ttgaacagca
     1921 aggtattcaa caatacgctc ttttactttt tccaagccat aatggtcatc gtccaatatt
     1981 ttttcagcag ccaataagtc tttgcttatc ttcgtttttt tcttccacgg aagattgata
     2041 agcgtatcaa tataattgcg tacaaccgaa gcttcggccg acatcggcga catcatcttt
     2101 aattttttaa gttcactcgc tactttagca agcgcctctt ttggcatatg tgcatcttgg
     2161 atacgctttt caagctcatc catttcagca ttttcatcct gttcacccag ctccttttga
     2221 atggccttaa cttgctcatt caaataatat tcccgttggg ttttttccat ttggcgcttc
     2281 acacggccac gaatgcgttt ttccacttga agtatatcta tttcaccttc catcaggcga
     2341 agcaagtgct ctaaccgttc agcaacgcta aacatctcaa gcactttttg tttttcatca
     2401 agttttaacg tgagatgtgc agcaatcgta tcggctaagc gacccgcatc atcaatcgta
     2461 gccagggaag tgagaatttc aggaggaatt tttttattca atttcacata ttgatcaaac
     2521 tgtgcaaaca cagtacgcat aagcgcttga atttcaatgt cgtccactga ctctgctatc
     2581 agttcaacgt gtgcggcaaa gcactcttct gtttcagtaa aaccagaaaa cttagcgcgc
     2641 tgtacgccct caactagtac ttttactgtg ccgtcaggca atttaagcat ctgtagcact
     2701 gtggcaacag ttcctacttc atacaagtct tctgcgctcg gctcatcttt gttggctgat
     2761 ttctgggcaa ccaatagaat ttgcttatca ccctcgga
//
LOCUS       DAOQSI010000207         2778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_42773_length_2778_cov_4.535439, whole genome shotgun sequence.
ACCESSION   DAOQSI010000207 DAOQSI010000000
VERSION     DAOQSI010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42773_length_2778_cov_4.535439"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1219
                     /locus_tag="PKL42_09895"
     CDS             <1..1219
                     /locus_tag="PKL42_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNU67426.1"
                     /translation="LDELELTDSKQVIPHFCGDAMLPDNLMRAGILNPHCCGLVTLTD
                     NDEVNLAVAIAVKLLNPKLPVVARAERDDIAANMASFGTDHIINPYTLFGDQLAMRVH
                     ALGTYLLHEWLTGITGETLLPPEPPPRGRWIVCGYGRFGKSVVANLEREHITTTIIEA
                     RPELTGCKHCIVGSGTEAKTLKEAGIETAVGIVAGTDNDINNLSIVMTAFELNPNIFL
                     VIRKNNSHNAALFKQFDAHITMQPTYIIAHECLAHLISPLLAQFLTLVRDQSNEWANQ
                     LISQLVSKVGEEVPETWAVTINQQSAPAVTELIRQHIAITLQMLSRQPTDREQVLDIV
                     PLMLLRNQQPMLVPALDTPLMMGDQILFCGRPEAKTAVTTLLEHDKSLIYVVDGSETP
                     NSLVFRWLQAKKI"
     gene            complement(1252..>2778)
                     /gene="mutL"
                     /locus_tag="PKL42_09900"
     CDS             complement(1252..>2778)
                     /gene="mutL"
                     /locus_tag="PKL42_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNU67427.1"
                     /translation="RASSEKHAWRIASNGSEISPIEPAALDAGTIVEVSDLYFNTPAR
                     RKFLKTEATEFGHCEAVFERVALSRPDVGFLLQHNGRVLSRLAVAVPERRFLDVLGEE
                     FVAESFHLDESAAGLRLWGRAAKPTFNRHSRDTQYVYVNGRFVRDKLISHAIRQAYQD
                     VLHHDRHPAFVLFLELDATLVDVNVHPAKTEVRFRDGQAIHRFIFHKLHKALATPTGV
                     LNASSIEHAPHQPHNTQQTYPTYQSQIPLSARESDSFYQTLYGNSNAENNHQMHQTQT
                     NTPEAFIYKASSDANFSRQENTTDLQDFSQDFSQEFPLGFAVAQIHGVYVLAQNVHGL
                     VVVDMHAAHERIMYEKLKNALDMQAVAMQPLLLPVSFHADKLEVAIVQETLAQHENSL
                     QKLGFDIAVLSPSTLAVRGVPVMLQHADAVTLARDVLRDLREFGASRALTERRNELLG
                     TMACHAAVRANRSLTIPEMNALLRDMEATERSGQCNHGRPTWFQVSLSDLDKMFMRGK
                     "
BASE COUNT          737 a          661 c          651 g          729 t
ORIGIN      
        1 cctggacgaa cttgaactca ccgatagcaa acaagtgatt ccacattttt gtggtgacgc
       61 gatgctcccc gacaatttaa tgcgtgctgg catcttaaac ccacactgct gtggcttggt
      121 gacactcaca gacaatgatg aagttaattt ggcggtggcc attgccgtga aattattaaa
      181 cccaaagcta cctgtggttg cgcgcgcaga acgtgatgat attgcagcca atatggcctc
      241 atttggtacg gaccatatca ttaaccctta cacattattt ggtgaccaac tggcgatgcg
      301 tgtacacgcg ttgggcactt atctattgca tgagtggctc acaggcatca cgggggaaac
      361 cttgctgccg cccgagccgc cacccagagg acgctggatt gtgtgtggtt atgggcgctt
      421 tggcaagtca gtggtggcta atttggaacg cgagcatatt actacaacca ttattgaagc
      481 caggcctgaa ctcaccggct gcaagcattg cattgtgggt agcggcactg aggccaaaac
      541 cttaaaagaa gcaggcatag aaactgcggt aggtattgtt gctggcaccg ataacgacat
      601 caataattta tcgattgtga tgacggcttt tgagcttaat ccgaatatat ttttagtcat
      661 tcgtaaaaat aacagccata acgcagcact ctttaaacag tttgatgcgc acattaccat
      721 gcagcccact tatattattg cgcacgaatg tttagcgcat ttaatttcac cattgctggc
      781 gcagttttta accttggtgc gtgatcaaag taacgagtgg gcaaatcaac tgattagcca
      841 actggtgtca aaagtgggtg aagaagtgcc agagacttgg gcagtaacca tcaaccagca
      901 atcagcgccg gcggtgacag aactgattcg tcagcatatt gccattacgt tgcaaatgtt
      961 atcaaggcaa cccacagacc gtgagcaggt gctggatatc gtgccgctga tgctgttgcg
     1021 caatcaacaa ccgatgttgg tacccgccct tgatacgcca ctgatgatgg gtgaccaaat
     1081 tttattctgc gggcggccag aagctaaaac tgcagtcaca accttacttg aacacgataa
     1141 gtcgctcatt tatgtggtgg atggcagcga aacccccaac agcttggtat ttcgctggct
     1201 acaagccaaa aaaatatagt gtgagttcgg ttgttgttat gcaaaatctt attacttgcc
     1261 tcgcataaac attttatcta aatcagatag actgacttgg aaccaggttg ggcgaccatg
     1321 attacactgc ccagagcgct ctgtggcttc catatcgcgt agtaacgcat tcatttccgg
     1381 aatagtgaga ctacggtttg ctctcacggc agcatggcag gccatggtgc caagcaactc
     1441 gtttcggcgc tcggtgagtg cacggcttgc accaaattca cgtaaatcac gcagcacatc
     1501 gcgtgcaaga gttaccgcat ccgcatgttg caacattaca ggcacgcctc gaactgccag
     1561 cgtgctgggt gagagtactg caatgtcaaa cccgagtttt tgcaggctat tttcatgttg
     1621 cgcaagggtt tcttgcacaa ttgcaacctc tagcttatct gcatgaaagc tcactggcag
     1681 cagtaagggc tgcattgcaa ctgcttgcat atcaagtgca tttttgagct tttcatacat
     1741 aatgcgctca tgcgctgcgt gcatatcgac gaccactagg ccatgcacat tttgcgctaa
     1801 cacgtaaaca ccatgaattt gcgccacggc aaatccaagt ggaaattctt gcgaaaaatc
     1861 ttgcgaaaaa tcttgtaaat cggtggtgtt ttcctgccga gagaaatttg catcgctgga
     1921 agccttataa ataaaggctt ccggggtatt ggtttgggtt tgatgcattt gatggttatt
     1981 ttcagcgttt gaattaccgt ataaagtttg gtaaaaactg tcagattcac gcgcgctcaa
     2041 tggtatttgt gactgatacg ttgggtatgt ttgttgtgta ttgtgtggct gatgtggcgc
     2101 atgttcaatg ctgcttgcat tcaaaacgcc cgtcggtgtt gctagcgcct tatgcagttt
     2161 atgaaaaata aaacgatgaa tggcctgacc atctctaaaa cgcacttcag ttttagctgg
     2221 atgcacattc acgtccacca acgtggcatc gagttcaaga aacaacacaa atgccggatg
     2281 gcggtcatga tgcagtacat cttgataagc ttgacgtatc gcatgagata tcagtttatc
     2341 gcgcacaaaa cggccattca catacacata ttgtgtatcg cgactgtggc gattaaaagt
     2401 cggtttggca gcccttcccc acaaacgcaa gcctgccgca gattcatcca agtgaaaaga
     2461 ctccgccaca aattcttcac ctaacacatc taaaaaccga cgctctggta ccgctacggc
     2521 taagcgactt aacacacgac cattgtgttg caataaaaat ccgacatctg gccgcgagag
     2581 cgcaacgcgc tcaaaaaccg cctcacaatg cccaaattct gtggcctcag tctttaaaaa
     2641 ttttcgcctt gctggcgtat taaaatacaa atcgctcact tctacaatgg tgccagcatc
     2701 tagcgcagcg ggttcaattg gtgaaatctc actgccgttt gaagcaatac gccatgcgtg
     2761 cttttctgag ctggctct
//
LOCUS       DAOQSI010000208         2742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_43623_length_2742_cov_6.563826, whole genome shotgun sequence.
ACCESSION   DAOQSI010000208 DAOQSI010000000
VERSION     DAOQSI010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43623_length_2742_cov_6.563826"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PKL42_09905"
     CDS             complement(<1..447)
                     /locus_tag="PKL42_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNU67428.1"
                     /translation="MKKIFQAPIAKPIYLFLLGASNVLGFAPYYLFPASILSVAVLFY
                     FWQKASSAKAAFWLGLQYGLGLFIIGIYWIYISLHDFGGMPWWFAGFVTFGLCVFMAL
                     FTATVGWLSKKSGALLLCAPLLWGIADWVRSWIFTGFPWLTLGYSQV"
     gene            660..1076
                     /gene="mscL"
                     /locus_tag="PKL42_09910"
     CDS             660..1076
                     /gene="mscL"
                     /locus_tag="PKL42_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNU67429.1"
                     /translation="MSMLSEFKEFALKGNVMDLAVGVIIGAAFGKIIDSFVGDIVMPI
                     ISSFGSVNFTDKFIALSEVPAGTAATLAAHKAAGVNTLAYGNFITISLNFIILAFVIF
                     QMVKFMNKLKKAEAAAPAPTPDDIVLLREIRDALKK"
     gene            complement(1147..2004)
                     /locus_tag="PKL42_09915"
     CDS             complement(1147..2004)
                     /locus_tag="PKL42_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HNU67430.1"
                     /translation="MAAEPEKPHNNKPSLLERLSHFLLREPEDREQLVSLLHGAYENH
                     LMDADSLAMIEGVLQVSEMQVRDIMIPRSQMDVIDITQPPEAFIPHVIETAHSRFPVI
                     EDDKNDVIGILLAKDLLRYYAGEDFEVRDMLRPAVFIPESKRLNILLKEFRSNRNHIA
                     IVVDEYGGVAGMVTIEDVLEQIVGDIEDEYDYDEDEDNIIQNADGQYRVKALTEIADF
                     NEIIGTDLSDEEFSTIGGLVVHQFGHLPKRNEETTFNGLHIKVLRADSRRLHSIMIEV
                     LPQETEESN"
     gene            complement(2013..2450)
                     /gene="ybeY"
                     /locus_tag="PKL42_09920"
     CDS             complement(2013..2450)
                     /gene="ybeY"
                     /locus_tag="PKL42_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNU67431.1"
                     /translation="MPTLHLTLQIASQLSNIPSKSQFKKWANAALRVDTEVTIRIVDE
                     DEGRALNNTYRGKDYATNVLTFPITETPHLMGDIVMCAPVVATEALAQHKSIEAHYAH
                     LTVHGILHLHGYDHEIAAQAELMEALETAIISKLGYANPYLIT"
     gene            complement(2604..2729)
                     /locus_tag="PKL42_09925"
                     /pseudo
     CDS             complement(2604..2729)
                     /locus_tag="PKL42_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
BASE COUNT          784 a          569 c          595 g          794 t
ORIGIN      
        1 cacttggctg taaccaagcg tgagccacgg aaaaccggta aaaatccagc tgcgaaccca
       61 atcagcaatg ccccataata atggtgcgca caataataaa gcgcccgatt ttttactcag
      121 ccagccaacc gtagcggtaa ataatgccat aaagacgcat aaaccaaagg taacaaaacc
      181 ggcaaaccac cagggcatgc cgccaaagtc atgcaggcta atgtaaatcc aataaatgcc
      241 gatgataaat aaccctaggc catattgcag gcctagccaa aatgccgctt ttgcgctgct
      301 ggccttttgc caaaaataaa ataaaacggc aaccgataaa atgctcgcag gaaacaagta
      361 atagggtgca aagcccagca cattgcttgc gcctaataaa aacaggtaaa tgggtttggc
      421 aatgggcgct tgaaatattt ttttcatggc tgaattgtag ctggatattg aaaaattgcc
      481 gcgagaattt aaaaagttgc ttgatgggtt ttaacaagaa tttacattat tcacggccat
      541 gttggaacgc gatgattgac atggcacaag caaacgcact atattaagcc cacaatgaga
      601 acactcaatt gtgcagttaa aaaaacatgt gatttatttc aattttttgg gagaacaata
      661 tgagtatgct atcagaattt aaagagtttg cactcaaggg taatgtaatg gatttggccg
      721 ttggtgtcat tatcggcgcg gcatttggca agattatcga ttcgtttgta ggcgacattg
      781 taatgccgat tataagtagt tttggttcag tcaattttac tgacaagttt attgcattat
      841 cagaagttcc agcaggtact gccgctactt tggcggcgca taaagctgcc ggggtaaata
      901 ctttagctta cggtaacttc attacaatct ctttaaattt tattatttta gcttttgtga
      961 tttttcaaat ggttaagttt atgaataaat tgaaaaaagc tgaagctgca gcacctgcac
     1021 caacgccaga tgacatcgtg ttattacgtg aaattcgtga tgctttgaaa aaataagcca
     1081 agaattttat taggcagtag aaaaaataaa agggctgaaa agccctttta ttttgcctga
     1141 ttttatttaa tttgattctt cagtttcttg tggcaacact tcaatcatga tgctgtgtag
     1201 ccgccggcta tctgcacgta ataccttgat atgtaagcca ttaaaggtgg tttcttcgtt
     1261 gcgttttggc aaatgaccaa attgatgcac caccaaaccg ccaatggttg aaaactcttc
     1321 atcgcttaaa tctgtgccaa taatttcatt aaagtcggca atttcagtga gcgcttttac
     1381 gcgatactgg ccatcggcat tctgaataat attgtcttca tcttcgtcgt agtcgtattc
     1441 atcttcaata tcgccaacaa tttgttccag cacgtcttca atcgttacca tgcccgctac
     1501 gccgccgtac tcatccacca caatggcaat atgattacga ttgctacgaa actctttgag
     1561 taaaatattc aagcgcttag attctggaat aaacacagcc gggcgtaaca tatcacgcac
     1621 ctcaaagtct tcgcccgcgt aatagcgtaa caaatctttg gccagcaaaa taccaatcac
     1681 atcatttttg tcgtcctcaa tcacaggaaa gcgcgagtgc gcggtttcga taacatgcgg
     1741 aataaaagct tctgggggtt gcgtgatgtc aatgacatcc atttgtgagc gcggaatcat
     1801 gatgtcgcgc acttgcattt cagatacttg caacacaccc tcaatcatgg cgagagaatc
     1861 agcgtccatc aggtggtttt catacgcgcc atgtagtagg ctgactaact gctcgcgatc
     1921 ttcgggttcg cgcagtaaaa agtggcttaa gcgctcgagt aagctgggtt tattgttatg
     1981 cggtttttcg ggttcggcag ccattaggcg tcctatgtga tgagataggg gttagcatac
     2041 cctaattttg aaattattgc agtctctaac gcctccatta attcagcttg tgcggctatt
     2101 tcgtggtcgt aaccgtgtaa atgcaaaatg ccatgcaccg ttaaatgcgc gtaatgtgct
     2161 tcaatggact tatgttgcgc caatgcttca gtggccacta cgggtgcaca catcacaatg
     2221 tcgcccatta aatggggtgt ttctgtgatg ggaaaagtca acacattggt ggcgtaatct
     2281 ttgccgcgat aggtattgtt cagcgcgcga ccctcatctt catccacaat gcgaatggtg
     2341 acttcagtat ccacacgcag cgccgcattc gcccattttt taaattgaga cttgctcgga
     2401 atgttgctca gttgagatgc gatttgtaag gtgaggtgca gcgttggcat gaggttttta
     2461 atgtattaaa aaaagcaaaa atagcctaaa aaaagcatat aacctttttc gatggtctgc
     2521 aagcctttat ttacaaggat cgcagggcag taaaattttc tcgggtaaat ttttgcttga
     2581 aattgaatgg tatgcatcaa tggttagtcg tggtgtttgg cttcatattc ttcataggca
     2641 ttgacgattt tttgcaccag tgggtggcgc accacatcgc ctgataaaaa gtgcgtcatg
     2701 gcgatgcctt taatttcttt aagcactttt tatcaggcga tg
//
LOCUS       DAOQSI010000209         2702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_44624_length_2702_cov_2.943710, whole genome shotgun sequence.
ACCESSION   DAOQSI010000209 DAOQSI010000000
VERSION     DAOQSI010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44624_length_2702_cov_2.943710"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="PKL42_09930"
     CDS             <1..414
                     /locus_tag="PKL42_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67432.1"
                     /translation="VKISTVDADTPGHKIEFYYPKQLSPEEREVARPQLDSIEPILAQ
                     SVLDELAARLNANKVTGAPLSYLRSLTNRAKAGQFMPEAGIRVAAAREQAKLEQIKKL
                     AEVIKPSNPSEIPKRLAAMHQVLARKSTSNLNQED"
     gene            417..2168
                     /locus_tag="PKL42_09935"
     CDS             417..2168
                     /locus_tag="PKL42_09935"
                     /inference="COORDINATES: protein motif:HMM:NF014581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HNU67433.1"
                     /translation="MQNLENKLNALVIDFLNQWIHYLGQFWPIVGVAFGFCISLAWLN
                     RDNKASGFFSGIAMLTLIFQLGFYFAIPQISLYLAATEFHLPDYYLWSFIGETVLGVF
                     MVWLIIRYGSPYIEMFKNKLTKTSRVERNKKTDIRQIGVHLPFAQSSYDPTKYFNYKK
                     GIFFGLNEHKKPVYLMWQQWRKSHVDVIGTTGSGKGVAAGVLLLQAVAAGECVVVVDP
                     KNDEWLPHVMHKAADDAGVPYVYIDLQSDQAQWNPIEHKNANEIEELLSAGFSLGEKG
                     SDADFYRLDDRRAARIFANYANRQDYSLIETLQLLIQHQPDIAEAGKKFVADLEEIGL
                     VASVNTKTGIDLGTLIKQGAVIYVRGSMRNPRILKLQRIFVLSMMQHIEVRPRELGNG
                     QKARHVCLFLDEFKYLISRPTLEALGAIRDKGAHVMIAHQSLGDLRDCPADLDPESVV
                     ASVNENCAIKMAYQVKDPDTALWLARMSGTILVDDEIRQVKTNAGLTETRDGERTLRQ
                     AERQLVDTNMLHALPERCAVLYGAGLAQFFFTSPIQLTKSSEAIKPASIPNAETKSLQ
                     NVAKPTLTTAQSMLDVD"
     gene            2158..>2702
                     /gene="mobC"
                     /locus_tag="PKL42_09940"
     CDS             2158..>2702
                     /gene="mobC"
                     /locus_tag="PKL42_09940"
                     /inference="COORDINATES: protein motif:HMM:NF041423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="HNU67434.1"
                     /translation="MLINSYQQRCERTQNKREMILRFLRDETWSNLTNLAGVLGLSEP
                     AAFKTLQQLERDGFSLRYKVSSLRLSLWGITPQGLAFAWAEDEAMQIRPYFEPGKLSV
                     MTIPHYLDIQRARLIAEAAGWTNWIPGNRLPKDIKKRPDAIALNPEGQTIAIELERTI
                     KTLKRYEAIFSVYLQQMKRDE"
BASE COUNT          824 a          607 c          577 g          694 t
ORIGIN      
        1 gtcaaaatat caacagtaga tgctgatact ccaggccata agattgaatt ttactatcct
       61 aaacaactaa gcccagaaga gcgtgaggtt gctagaccac aattggattc aattgagcca
      121 atactagccc agtctgtact agatgagtta gcagctcgac taaatgcaaa caaagtcaca
      181 ggcgcaccgc ttagctatct acgttcactc accaaccgtg caaaagctgg acagttcatg
      241 ccagaagcag gcatacgagt tgcagcggct cgtgagcaag ctaagttaga acaaatcaaa
      301 aaattggctg aagtaattaa gcccagcaat ccaagtgaaa taccgaaacg tctagcggcc
      361 atgcatcaag tacttgctcg caaatcaaca tcaaacttaa atcaagagga ctaaccatgc
      421 aaaacctaga aaataaactc aatgcacttg taatcgactt tttaaatcaa tggattcact
      481 acttaggtca attttggcct attgtcgggg ttgcgtttgg cttttgcatt tcgctagcgt
      541 ggctcaatcg cgacaataag gccagcggat tcttttctgg tatagcaatg cttaccttga
      601 tatttcagct tggcttttac tttgccattc cccaaatttc actttacctt gctgcgactg
      661 aatttcattt acctgattat tatctgtgga gctttattgg tgaaactgta ttaggtgttt
      721 tcatggtctg gctcatcata cgatatggct caccatatat tgaaatgttt aaaaacaaac
      781 tcacgaaaac atctagggta gaaagaaata aaaagactga cattcgccaa attggtgtgc
      841 atttaccatt cgcacaaagc agctacgacc caaccaaata cttcaactat aaaaaaggca
      901 tattttttgg tctaaatgaa cacaagaaac ctgtttactt aatgtggcaa caatggcgca
      961 aaagccatgt tgacgtcatt ggcaccacag gaagtggcaa aggtgtagca gcaggcgtct
     1021 tgctattaca agccgttgca gcaggtgaat gcgtcgtggt ggttgacccc aagaatgatg
     1081 aatggctacc ccatgtgatg cataaggcag ctgatgatgc aggtgtacct tacgtctata
     1141 ttgatttgca gtctgatcaa gctcaatgga atcccattga gcataaaaat gccaatgaaa
     1201 ttgaagagct tctttccgca ggattttcgc ttggagaaaa aggctcagat gctgactttt
     1261 accgacttga tgaccgacgt gcagcacgca tttttgccaa ctatgccaat cgacaagact
     1321 actcactcat tgaaacccta caactactca tccagcatca gccagatatt gcagaagcag
     1381 gtaagaagtt tgtagctgat cttgaggaaa tcgggttagt cgcatcagta aatacgaaaa
     1441 caggcattga tcttggtacg ctaattaaac agggggctgt catttatgtg cgcggctcta
     1501 tgcgcaaccc acgcatactt aaactacaac gtatctttgt gttatccatg atgcagcata
     1561 ttgaagtgcg accacgtgag ctaggcaacg gacaaaaagc gcgccatgta tgcttattct
     1621 tagatgagtt caaatatctc attagccgtc caaccttaga agcactaggt gcaattcgtg
     1681 acaaaggggc gcacgtcatg atcgcacacc agtcactcgg tgatcttcgc gattgccctg
     1741 ctgatcttga tcctgaatca gtggtagctt cagtcaatga aaactgtgcc atcaaaatgg
     1801 cttaccaagt caaagaccca gatactgctt tatggctggc gcgcatgagc ggcacgatac
     1861 tagtcgatga tgaaatacga caagttaaaa ccaatgctgg ccttacagaa acccgtgacg
     1921 gggaacgcac cttgcgccaa gccgagcgcc aacttgtgga taccaatatg ctgcatgcct
     1981 tgcctgagcg ctgcgcagtg ttgtatggcg cagggctagc gcaattcttt tttacttcgc
     2041 caattcaact cactaaaagt agtgaagcaa ttaagcctgc atctattcca aacgctgaaa
     2101 ccaaatcatt acaaaacgtg gctaaaccga ccctcaccac agcacagagt atgctcgatg
     2161 ttgattaact cctaccaaca acgttgtgaa cgtacacaaa acaaacgtga gatgattcta
     2221 cgttttttaa gggatgagac ttggagcaat ctgacaaacc tggctggtgt gctaggtttg
     2281 tcagaacccg cagcatttaa aactctacaa cagctagaac gtgacggatt ttcactacgt
     2341 tacaaagttt cttcattacg gctaagctta tggggcatta ctccacaagg cttggcgttt
     2401 gcatgggctg aagatgaagc catgcaaatt agaccttatt ttgaacctgg caaactatca
     2461 gtcatgacca ttccgcacta cttggatatt caacgggcaa gactgattgc tgaggctgct
     2521 ggctggacaa attggattcc tggcaatcga ctacctaaag atattaaaaa acgtccagat
     2581 gcaattgcac taaacccaga aggccagacg attgcgattg aactggagcg cactattaag
     2641 actttgaagc gatatgaggc aattttttca gtttacctgc aacagatgaa aagagatgaa
     2701 ta
//
LOCUS       DAOQSI010000210         2653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_45860_length_2653_cov_5.698229, whole genome shotgun sequence.
ACCESSION   DAOQSI010000210 DAOQSI010000000
VERSION     DAOQSI010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45860_length_2653_cov_5.698229"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PKL42_09945"
     CDS             complement(<1..183)
                     /locus_tag="PKL42_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67435.1"
                     /translation="MKLGLKRAHYLLMLAGISLNAPIAFAENGNATAAETQKSTTLSS
                     ASDAAAAAATAASAAAL"
     gene            complement(515..2419)
                     /locus_tag="PKL42_09950"
     CDS             complement(515..2419)
                     /locus_tag="PKL42_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNU67436.1"
                     /translation="MLDSIRSVAQGWVGKALLALITIPFALFGIDSYLSSAGSNVAIA
                     KVGKNNISIQAYDNALKNIRNRLQSEGKIDPAAPDLPEMKSFALNQLINDQLLDDEVK
                     HANFAISDMQLTTYITGMPEFQKDGKFSQELYDQTLTDAKLTPNKFEARIRGDLLAQQ
                     AQENFAKLGFISNARAEQAYQLTNQQRVVAVAEIKTKDFVDQVKIEPAQVRAYYDQHK
                     AKLMHSEQVKIEFLLLSAAQLITGVGVDDAEVRKFYDDNIDNIKAKEQRRASHILIGF
                     GVNPTPEKKAEAKAKAEGLLAEIKKAPKTFEALAVKNSQDDESASKGGDLGVQGRGYF
                     TPPVEKAIYDLKVNEVSNLVESEYGYHIIKVTEVIGENTDFETLKPQIKGDLMFKKAQ
                     DEFIKQAEGFSELVYTQSNSLAPAAKAFGSEVQKSDWLTRESAAKFFKDNSKFVDLIF
                     SVESLKDRRNTAAMEVSPNNLVSARVVDYKPATQKSFDEVKEGIEALLKLEAAAKLAS
                     TKGEAALKDLRAGKSVADLQWTSEVAVDRKNAEGLTELAMTQAFKTDVSKLPAYSGLA
                     DKQQGYLLVKVLEAKTLKSEDPAAEKLAQNELETALNSEYLAAYKQSLREKAKVTVNE
                     KLLLGSTENN"
BASE COUNT          744 a          598 c          480 g          831 t
ORIGIN      
        1 taaggctgcg gcactagcag ctgtcgctgc cgctgcagcc gcatctgaag cagaagataa
       61 tgttgtactt ttttgcgttt ctgctgctgt tgcattacca ttttctgcaa aagcaatcgg
      121 agcgttcaaa ctgatgccag caagcatcag caagtagtga gcgcgtttta gccctagttt
      181 cattccatcc ctctgtgttc taaattgctt gccaattgtc cgcgcaggcg cttaaaccct
      241 agcaatacgt taattttttt agagtatata gcaaaaattt acaattacca taattttagg
      301 caaaattgag acactaaaaa attagctaaa aaacgtacaa aatcgaacaa accttttacg
      361 ctgctctgca agcctttatt tataaggctc gcagggcatg ttttttctct cgaggaattt
      421 aatctgcgaa ttaacccatt ttatgtgggc taatctgcga atgaaagcgt gaaattgttg
      481 caaaaaacca acaaaagcaa gcagtaaaaa aaagttaatt attctctgtg gatccaagca
      541 aaagcttttc attgaccgta actttcgctt tttcacgcaa tgactgctta taagcagcaa
      601 gatattcgct gtttaatgca gtttctaatt cattttgtgc taatttttca gccgctggat
      661 cttccgattt caaggttttt gcctcaagca cttttaccag caagtagcct tgttgcttat
      721 cagccaagcc cgaataagcc ggtaattttg atacatctgt tttaaaagcc tgcgtcattg
      781 ctaactcagt taagccttcg gcattttttc gatctaccgc aacttcagat gtccactgta
      841 aatctgccac tgatttaccg gcgcgtaagt ctttaagcgc tgcctcacct ttcgttgagg
      901 ctaattttgc agccgcctct aatttaagta aagcttcaat gccctctttt acttcatcaa
      961 agcttttttg cgttgcaggt ttataatcaa ccacacgtgc agagactaga ttatttggtg
     1021 aaacttccat tgccgctgta ttgcgacggt ctttcaacga ctccactgag aaaattaagt
     1081 ctacaaactt gctattatct ttgaaaaact tagcggctga ttcacgtgtc agccagtccg
     1141 atttctgaac ttcactgcca aatgctttag cggctggcgc taggctgttg gattgcgtat
     1201 aaaccagctc actgaacccc tcagcctgtt taataaattc atcttgcgct ttcttaaaca
     1261 ttaagtcgcc tttaatttgc ggttttaatg tttcaaaatc cgtgttttca ccaatcacct
     1321 cggtcacttt aataatatga taaccatact ctgactcaac cagattgctc acttcattca
     1381 cttttaagtc ataaatcgcc ttttcaacag gcggcgtgaa ataaccacgg ccctgcacac
     1441 ctaaatcacc gcctttcgaa gcagactcat catcttgcga atttttaacc gcaagcgctt
     1501 cgaatgtttt tggtgccttc ttgatttcag ctagcaaacc ttctgctttc gccttggctt
     1561 ctgctttctt ttctggtgtt gggttaacac caaaaccaat caaaatgtgg ctcgcacgac
     1621 gttgttcttt ggctttgata ttgtcgatat tatcgtcata aaatttacgc acttcagcgt
     1681 catcaacacc caccccagtg atgagttgcg ctgcagacaa tagtaagaat tcaattttaa
     1741 cttgctcaga atgcatcaac tttgctttat gctgatcgta ataggctctg acttgtgctg
     1801 gctctatctt cacctggtca acaaaatctt ttgttttaat ttcagctacc gctaccacgc
     1861 gttgctgatt agtcaattgg taggcttgtt cagcccgtgc atttgaaata aaacccaact
     1921 tagcaaagtt ttcttgcgcc tgttgtgcta ataaatcgcc acggatacgt gcttcaaact
     1981 tattaggtgt caatttggca tccgtaagcg tttggtcata aagctcttgt gaaaacttac
     2041 cgtctttttg aaactccggc atgcctgtga tgtaggtcgt taactgcata tcactaatgg
     2101 caaaatttgc gtgtttaact tcatcatcca gcagttgatc attgatgagc tgattgagcg
     2161 cgaatgattt catttctggc aggtcaggtg ctgcaggatc aattttccct tcagattgca
     2221 gacgattgcg aatattctta agtgcgttat cgtacgcttg aatcgaaata ttatttttac
     2281 ccacttttgc tatcgccaca ttgctacctg cactacttaa atatgagtca atgccaaaca
     2341 atgcaaatgg aattgtaatg agtgccaaca aagccttgcc tacccaacct tgagcaaccg
     2401 aacgaatact atctaacata acgcatttac ctttaacata aattctgaaa ttttaggact
     2461 ggcaattaag cttgagctta ctaagtgaca tgcaaaccaa accaaataaa gtccaaattt
     2521 tgggatacga gactatagca tatttctaat attgcataag ctacgaaacg ctcggacaaa
     2581 gcatgagcga ataagtttta acgcataaaa acaaaaaagc aagacatgaa actgaataaa
     2641 caagaactgg aag
//
LOCUS       DAOQSI010000211         2609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_47064_length_2609_cov_3.828504, whole genome shotgun sequence.
ACCESSION   DAOQSI010000211 DAOQSI010000000
VERSION     DAOQSI010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47064_length_2609_cov_3.828504"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /locus_tag="PKL42_09955"
     CDS             complement(<1..165)
                     /locus_tag="PKL42_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNU67437.1"
                     /translation="MFNLASTLSSLSTLSWIILTSLIGGLLSVSLAALFALNVRITWV
                     PMLVSYAIGAM"
     gene            complement(304..1050)
                     /gene="pgeF"
                     /locus_tag="PKL42_09960"
     CDS             complement(304..1050)
                     /gene="pgeF"
                     /locus_tag="PKL42_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HNU67438.1"
                     /translation="MMSQLDFIIPNWPVPANVVALQTTRLGGVSGAPYASLNLGAHVN
                     DDPIAVAKNRQLLSPYLPNEPVWVNQVHGIEVIDAAQSACLQNADASFTTKPNVVCVT
                     MTADCLPVLLCDKAGTVVAAVHAGWRGLCDGVIEAAVNKLPVEHSEILAWLGPAIGPN
                     AFEVGSEVMAQFIAKDAQAASAFQTNGNKWLGDIYQIARQRLSYLGVTQIYGGSVNED
                     FCTYTDATRFYSFRRDDVTGRMASLIWLAS"
     gene            complement(1051..2148)
                     /gene="rluD"
                     /locus_tag="PKL42_09965"
     CDS             complement(1051..2148)
                     /gene="rluD"
                     /locus_tag="PKL42_09965"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HNU67439.1"
                     /translation="MTDATQQSLSNNLTLTIPHDLGGLRLDVALQRLLPEHSRSRLQA
                     WVKEGLVTVDAKTSTSKTKVWGGEQVLVQVQTKPEITAFAAEDIPLHVVFEDEHILVI
                     NKPAGMVVHPAAGNWSGTLLNALLFHAPQLKDVPRAGIVHRLDKDTSGLLVVAKTLAA
                     QTNLVRQLQARTVKREYRAIVWGQLWRNGVVDQPIGRDPRSRTKMAINRMGKPAITRY
                     EILERFSVQTYLRLNLETGRTHQIRVHMQFLKAPIVGDPVYGFRNIVPIRAMTQTLRD
                     AVSQFNRQALHAIKLGLMHPATNEFVEWQIELADDMKALLEAMRHEDPRDEDEEAFEF
                     SEAPYLADEDYEEDGDYDDEDDEDFDMDDEA"
     gene            2187..>2609
                     /gene="bamD"
                     /locus_tag="PKL42_09970"
     CDS             2187..>2609
                     /gene="bamD"
                     /locus_tag="PKL42_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNU67440.1"
                     /translation="MKYILILMITLFLNGCTIFGAPTELDDTKGLSAERIYQMGEEKM
                     IDKDYEKAIVYFGKLESRYPNGRYATQAQLETAYAHYKKGDAVQAVAAAERFIKLHPN
                     HPNVDYAYYLKGLAVFNERGLLEKLTKQEISDRDPRALR"
BASE COUNT          709 a          663 c          559 g          678 t
ORIGIN      
        1 catcgcacca atagcataac tcaccagcat tggcacccat gtaatacgca cattcagcgc
       61 aaataatgcc gctaaagata cactcagcaa cccgccaatt aggctggtga gtattatcca
      121 cgagagtgtg gataatgatg acaaagtgct agcgaggtta aacatggggt actttattgt
      181 atgctgatgt tgtcaaattg gacgtttgaa gtgcgtatta taaacgagca atgacagtac
      241 cgtaatttat tgctggtaaa aatggcttcg gcagaccgca aacgtctgca atggcagtaa
      301 atgttaactt gcaagccaaa tcaaacttgc catacgacca gtgacgtcat cacgcctaaa
      361 agaataaaaa cgtgtagcgt ctgtataagt gcagaaatct tcattcacac tgccgccgta
      421 aatttgcgtc actccaagat aacttaagcg ttggcgtgct atttgataaa tatcgcccag
      481 ccatttgttg ccatttgttt gaaaagcact cgcagcctgc gcatcttttg caataaattg
      541 cgccattacc tcactaccca cttcaaaagc atttggccca atcgcaggcc caagccaagc
      601 gagaatttca ctatgttcta cgggcaattt attgactgcg gcttctatca caccatcaca
      661 caagccgcgc cacccggcat gtacagcagc cacaacagtg cccgctttgt cgcaaagtag
      721 caccggcagg caatcagccg tcatcgtcac acacactacg tttggcttgg tggtaaaaga
      781 cgcatcggca ttttgtaaac aagcgctttg tgcagcatca atgacttcaa taccatgcac
      841 ttgattgacc catactggct cgttcggtaa ataagggctt aataattgtc gatttttggc
      901 taccgctatc gggtcatcgt tgacatgcgc ccctaagttc aaacttgcat agggcgcacc
      961 gcttacaccg cccaagcgcg tggtttgcaa ggcgactaca ttcgcaggca ctggccaatt
     1021 aggaataata aaatctaact gtgacatcat ttatgcttcg tcatccatgt caaaatcttc
     1081 gtcatcttca tcgtcgtaat ccccatcctc ctcatagtct tcatcggcaa gatatggcgc
     1141 ttcactaaac tcaaacgctt cttcatcttc atcacgcgga tcttcgtggc gcatggcctc
     1201 aagcaatgct ttcatgtcat cggctaattc tatttgccat tccacaaact cattggtggc
     1261 cggatgcatc aaaccgagct tgattgcatg cagtgcttgc ctattaaatt ggcttacagc
     1321 atcgcgtaac gtttgtgtca ttgcacgaat tggtacaata ttacgaaatc cgtaaacagg
     1381 gtcgcccaca atcggtgctt ttaaaaactg catatgcacg cgaatctgat gcgtgcggcc
     1441 agtttctaag ttcaaacgca aataggtttg cacagaaaaa cgttctaata tttcataacg
     1501 cgtaatggca ggcttaccca tgcggttaat cgccattttg gtacgcgaac gcgggtcacg
     1561 gccaatcggc tgatctacca cgccattacg ccacaattgc ccccaaacaa tggcgcgata
     1621 ttcacgctta acggtgcgcg cctgcaactg tcgcactaag ttggtttgcg cggccaaggt
     1681 ttttgccacc accaacaagc cgctggtatc tttatctaag cgatgcacaa tgcccgcacg
     1741 cggcacatct ttgagttgcg gcgcatgaaa caataaagcg ttcaacagtg taccactcca
     1801 attgcctgct gcgggatgca cgaccatacc cgctggttta ttgatgacta aaatatgttc
     1861 atcttcaaaa acaacatgta acggaatatc ttcagcggca aacgcagtga tttccggctt
     1921 ggtttgcacc tgcaccagca cttgctcgcc accccacact ttggttttgg acgtactggt
     1981 tttcgcgtcc actgtcacca agccttcttt tacccaggcc tgtaaccgtg agcgagaatg
     2041 ttcaggtagc aatcgttgta gggcaacatc taggcgtaaa ccgcccaaat catgcggaat
     2101 tgttagtgta aggttgttac taagagattg ttgagtcgcg tctgtcatcg gttataatgg
     2161 gtgataattc tttgattggg tgttgcatga agtatatctt aattttgatg attaccttat
     2221 tcctcaatgg ttgtaccatt tttggcgcac ccactgagtt agatgatacc aaaggcctgt
     2281 ctgctgagcg catttaccaa atgggcgaag aaaaaatgat cgataaagac tatgaaaaag
     2341 ctatcgtcta cttcggcaaa ctagaatcgc gctaccctaa cggccgctac gccacacaag
     2401 cccagcttga aaccgcctac gcgcattaca aaaaaggcga cgctgtgcaa gcagtggccg
     2461 cagcagagcg ctttattaaa ttgcatccta atcatccaaa cgtggattac gcctattatt
     2521 taaaaggcct agcggttttt aacgagcgcg gcttgcttga aaaactgacc aaacaagaaa
     2581 ttagcgatcg cgacccgcgt gcattgcgt
//
LOCUS       DAOQSI010000212         2588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_47677_length_2588_cov_5.082906, whole genome shotgun sequence.
ACCESSION   DAOQSI010000212 DAOQSI010000000
VERSION     DAOQSI010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47677_length_2588_cov_5.082906"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..459)
                     /locus_tag="PKL42_09975"
     CDS             complement(154..459)
                     /locus_tag="PKL42_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNU67441.1"
                     /translation="MKTTDPDLMITESSGNVFADLGFNDAEATILAMRAQLMCDIAKQ
                     IDKHQWTQSEAAKRFGVSQARVSDLVRGKFDKFSLDTLIQLATRAGHSVRIELAEAA"
     gene            complement(463..777)
                     /locus_tag="PKL42_09980"
     CDS             complement(463..777)
                     /locus_tag="PKL42_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNU67442.1"
                     /translation="MKPIKFVGSSLDDLRDFPQEARSQCGFELRRVQDGLMPTDFKPM
                     PSIGRGVYEIRVHELGEFRVIYIAKHEDFIYVLHSFQKKTQATRKEDIDLAIKRNKLI
                     GE"
     gene            848..2032
                     /locus_tag="PKL42_09985"
     CDS             848..2032
                     /locus_tag="PKL42_09985"
                     /inference="COORDINATES: protein motif:HMM:NF016491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HNU67443.1"
                     /translation="MFNYIGGTFFSFVDAYVNSISAALSLALIGVVTAALTVYYLFVG
                     YAVMRGEVHAPLVTIVWKSVKMNLILYIALSAGVYQTFVVVNVGDVMAQAMTSISNAS
                     SASAGATVCVPAGTGTPAVLSVIDCNFEKLLVPYDSIKTAAKGIDWYDVPSALSLEVS
                     SGLYSLCLMVLVLAVAYTLFTTQLTLSLVLAVGPLFIAALAFEPTKSYFEGWKNKIVY
                     VLILNLFIVIFLGLVFIIVNGYIIHLGFNVDRSADFIGVQQVEAIKSVLKLAAIMVLF
                     GYMFTKLDNIAHSLVGGGDNSGGLGLMLATAALGRQAWRALSSNVGSGGGTGAGGTIA
                     PAGGGFSGALGRGLQAAGQGAGAGALAIGQGMGAGALTVGQGTRSIARHVAHQISTRM
                     RQ"
     gene            2065..>2588
                     /locus_tag="PKL42_09990"
     CDS             2065..>2588
                     /locus_tag="PKL42_09990"
                     /inference="COORDINATES: protein motif:HMM:NF016238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HNU67444.1"
                     /translation="MMIEFAKRLVNSAKQAFTNDKTDIHQQARDWEASQTLALMQSES
                     RAWKIAGVSTMLTALSVTGLVAMLPFYKVVPMTFLVDKATGAAQLVDTSTAISMSSEQ
                     WDKHWVEEYVNSRERYNWMLLQYDHDKTMALSGDEIAKTYDALYQGANSIDKQLSVKL
                     HLNLTHQMHRILTHP"
BASE COUNT          693 a          559 c          602 g          734 t
ORIGIN      
        1 tgggtgggtc aatattcgat gcatttggtg ggtcaaattt agatgcaatt taacagtgct
       61 aattctggat cttcaatttt atttattttt aatctcattt ttcgaccaat tcagctaagt
      121 agggcgaggc ttaatgccaa acgcataaca gaattaagcc gcttccgcca actcaatacg
      181 cacgctatgt ccagcgcgcg tggcaagttg tatcagcgta tccaagctaa atttatcaaa
      241 tttaccgcgc accaagtcag aaacgcgggc ttgagataca ccaaagcgtt ttgcagcctc
      301 actttgtgtc cactgatgct tgtctatttg ctttgcgata tcacacatca attgcgcgcg
      361 cattgccaaa atagttgctt cagcatcatt aaaacccaaa tcagcgaaca cgttgccgct
      421 agattcggta atcatcaaat cagggtcagt tgttttcatg gtttactctc caatcaattt
      481 attacgttta atcgccaagt caatatcttc tttacgtgtg gcttgcgttt tcttttgaaa
      541 gctatgcaac acataaataa agtcttcgtg tttggcaata taaatgacac gaaactcacc
      601 aagctcgtgt acacgaattt cataaacgcc tctgcctata cttggcatgg gcttaaaatc
      661 cgtgggcatc aatccatctt gaacgcggcg cagttcaaaa ccacactgac tacgcgcctc
      721 ttgcggaaaa tcacgcaggt cgtcaaggct gctacctaca aatttaattg gcttcatgca
      781 aacgattata taagtattga tatagaaaat caagcaggat ttaaactaca acaattaaag
      841 gtacaccatg tttaattaca ttggcggtac attcttctca tttgttgatg cttacgtgaa
      901 tagcatctca gccgcactca gcctcgcctt aatcggtgtt gtgacagctg cgctgacggt
      961 ttattaccta tttgtaggct atgccgtcat gcgtggtgag gtgcatgcgc cactggttac
     1021 gattgtgtgg aaatcagtca agatgaacct gattttatac attgctttat cggctggcgt
     1081 ttatcagaca tttgtagtgg ttaatgtggg cgatgttatg gcacaagcga tgacgagcat
     1141 ttcaaatgca agctcagcaa gtgctggtgc tacggtatgt gtaccagctg gcactggcac
     1201 accagccgta ttgtcagtca ttgattgtaa ttttgaaaag ctactagtac cttacgacag
     1261 catcaagaca gccgccaaag gcatagactg gtatgacgtt ccatccgctt tatcgctaga
     1321 agtgtcttca ggtttgtatt ccctgtgttt aatggtgttg gtgttagcgg ttgcctacac
     1381 cttatttacc acccaactca ctttgtctct cgttttagcg gtcgggccat tatttatcgc
     1441 ggctttagct tttgagccga ctaaaagtta ttttgagggc tggaaaaata aaatagtgta
     1501 tgtcctgatt ctgaatcttt ttatcgtcat ttttttaggt ctcgtcttca tcattgtgaa
     1561 tggttacatc attcatttgg gctttaacgt ggatcgttcc gcagatttta tcggcgtgca
     1621 acaagtggaa gccattaaat cggtattaaa gcttgccgcc atcatggtgc tgttcggcta
     1681 tatgtttacc aaactcgaca acatagccca cagtttagtg ggggggggag ataactcagg
     1741 tggactcggt ttaatgctag ccactgcggc attaggcaga caagcttgga gagcattaag
     1801 cagcaatgtc ggttctggtg gcggaactgg tgctggggga acaatcgcgc cagcgggcgg
     1861 tggttttagt ggcgctttag gtcgagggct acaagctgct gggcaagggg caggcgcagg
     1921 tgctttagcc ataggtcaag gcatgggtgc aggcgcatta acggtagggc aaggtactag
     1981 aagtattgct aggcacgttg cccatcaaat ttccactcgc atgcgtcaat aaacctaact
     2041 ttaaatatta caagcacaag gacaatgatg attgaatttg caaagagact agtcaactca
     2101 gccaaacaag cgtttacaaa tgacaaaaca gatattcacc aacaggctcg agactgggaa
     2161 gcctcgcaaa cgcttgcgtt gatgcaatct gaatcgcggg cttggaagat cgctggcgta
     2221 tcgacaatgt tgacagctct aagcgtcacg ggcttagtgg cgatgctgcc attttataaa
     2281 gtcgtaccca tgactttttt agtggataaa gccaccggtg cagcgcaatt ggtagatacc
     2341 agcacggcca tctccatgag ttcagaacaa tgggacaagc actgggtgga agagtacgtc
     2401 aatagtcgtg agcgttacaa ctggatgcta ttgcagtacg accacgataa aaccatggcg
     2461 ttgtcagggg atgagattgc aaaaacttat gatgcgcttt atcaaggggc gaattccatt
     2521 gataagcagc taagtgttaa attgcatcta aatttgaccc accaaatgca tcgaatattg
     2581 acccaccc
//
LOCUS       DAOQSI010000213         2538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_49190_length_2538_cov_4.668546, whole genome shotgun sequence.
ACCESSION   DAOQSI010000213 DAOQSI010000000
VERSION     DAOQSI010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49190_length_2538_cov_4.668546"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2411)
                     /locus_tag="PKL42_09995"
     CDS             complement(<1..2411)
                     /locus_tag="PKL42_09995"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNU67445.1"
                     /translation="MIKSWFFGIFGFFFTRAAIAIASFAIVLLLILLPFFKNQLLAMV
                     ENQGQTFANTTIAATTSNLYTESYGDVIEYLLKVLKDTDNILFVVITTKANDEVVINK
                     NTWTQSQNVYHFNQTKLNPSKSALYYVEKNPVSHVNCFMYHQEIMIGGTSWGLLTVGM
                     SDAQYHQVKRKYSTIVGITSVGLVLVLLLLFYRNSKQFRAEMLQLTNTGLQLQRGDLA
                     ARAPEQAFGEIGTLSRAINTMAINLQEQTHRTQQLAHIVEQTNDAIILFDNAMQVVFV
                     NDAISEVTGYSPAFFQNMSMTTFTNILQLNLDEVLREVDFNNVHLQRSRTHDFVITKK
                     GQQITDVEVRLEFITNEQTHNQNFLVVLSNISERKRLEKELHQLAFYDKLTNLPNRRL
                     FMDRMRQLIESSKRNKKSFALFFMDLDNFKFINDTLGHEAGDEFLVQVGKRIKGIVRS
                     NDMVTRLGGDEFTVIVEDVKSGAHIDVGHLADKLIAGLAATPIYIQGRPLTISTSLGV
                     AIYPENGTDSDTLVRNADIAMYSAKKSGKNRHAFFSEAMNAALRERIEIEEDLKQAIR
                     LDNQITLYYQPIVDLATEQLVGAEALVRWHHPEKGYISPVDFIPIAESSNLIVVLSEY
                     LLNIAFKQAHAWRNLAFTPYMSVNISGRHFEKPNFIERLAYYLAVYKLDAGKIQLEFT
                     ESIMLGSTKDTIAKFQQLKQMGFKIAIDDFGTGYSSLGYIHQLPIDVIKIDRSFVIGL
                     LENTKTSAIVTAITTLSTTLGIKTVAEGVELEVHADRLRQYHCDYGQGYLYDKALAIA
                     AFEQK"
BASE COUNT          731 a          566 c          496 g          745 t
ORIGIN      
        1 tacttttgct caaacgccgc aattgctaag gctttgtcgt ataaatagcc ttggccgtaa
       61 tcgcaatgat attgccgcag tctatcggca tgaacctcca attcaacgcc ttcagccaca
      121 gttttaatgc ccaatgttgt tgaaagtgtg gtgatggctg tcacaatggc actggtttta
      181 gtgttttcta gcaacccaat tacaaacgag cgatcaattt taatcacatc aatcggcagc
      241 tgatgaatat agcccagcga tgaatagcca gtaccaaaat catcaatcgc gattttgaag
      301 cccatttgtt ttaactgctg aaatttcgca atggtatctt tagtggaacc cagcataatg
      361 ctttcagtaa attcaagctg aattttaccg gcatcgagtt tgtagacggc taagtaatat
      421 gccaagcgtt caataaaatt gggcttttca aaatgtcggc ccgaaatatt caccgacata
      481 tagggcgtaa atgcaagatt gcgccatgca tgcgcttgct taaaggcgat gttgagcaag
      541 tattcgctca ggacgacaat taaatttgag ctctccgcaa taggaataaa gtcaaccggc
      601 gaaatatagc ctttttctgg atgatgccag cgtactaagg cttcagcgcc caccagctgc
      661 tctgtcgcca aatccacaat gggttgataa tacagggtga tttggttgtc caatctaata
      721 gcctgtttta aatcctcttc aatttcaata cgttctcgta gtgcagcatt catggcctca
      781 gaaaaaaacg catgacggtt tttgccagat tttttagcag aatacatggc aatatcagcg
      841 ttcctcacca gcgtgtcact gtctgtgccg ttttcagggt aaatcgccac ccccaagctg
      901 gtgctgatgg ttaaaggcct gccttgaata taaatgggcg tagccgctaa cccagcaatg
      961 agcttatctg ctaaatggcc aacatcaata tgtgcgcccg acttaacgtc ttcaacaatc
     1021 acggtaaact catcaccacc aagccgcgtg accatatcat tgctacgcac aataccttta
     1081 atccgtttgc ccacctgcac taaaaattca tcgccggcct catggcccaa agtgtcatta
     1141 ataaatttaa agttatctaa atccataaaa aataatgcaa aggatttttt attccgcttg
     1201 ctagactcaa tcagctggcg cattcgatcc ataaataaac ggcggttagg caaattggtc
     1261 agtttgtcat aaaacgcgag ctgatgtaat tctttttcaa ggcgtttacg ttcagaaata
     1321 ttggataaca ccaccaaaaa attctgattg tgcgtttgct cattggtaat aaattccagg
     1381 cgcacttcca cgtctgtgat ttgctgacct tttttggtga tcacaaagtc atgcgtgcgc
     1441 gaacgctgaa gatgcacatt gttaaaatcc acctcacgca atacctcatc taaattgagt
     1501 tgcaaaatat tggtaaaggt tgtcatcgac atgttttgaa aaaatgcagg agaataccct
     1561 gttacttcgc tgatggcatc attgacaaac accacctgca ttgcgttatc aaacaagata
     1621 atcgcatcat tggtttgctc aacaatatgg gccagttgct gtgttcgatg cgtttgttcc
     1681 tgcagattaa tcgccatagt gttgatggct cttgataaag taccaatttc accaaaggcc
     1741 tgttccgggg ctctggccgc taaatccccc cgctgtaatt gcaagcctgt attggttaat
     1801 tgcaacattt ctgcccgaaa ctgtttagag tttcgataaa ataatagcag caacaccaat
     1861 accagcccaa ctgaagtgat gccgacaatg gttgagtatt ttcttttaac ctggtgatat
     1921 tgggcatcac tcatgccaac cgtcaaaagg ccccaacttg taccgccaat cataatctcc
     1981 tgatggtaca taaaacaatt cacatggctg acagggtttt tttcaacgta atagagtgcg
     2041 cttttactgg ggtttaattt tgtttgatta aaatggtaaa cattttgact ttgtgtccat
     2101 gtatttttat tgatgacaac ttcatcgttc gctttggtgg taatcaccac aaataaaatg
     2161 ttatcggtat cttttaacac tttgagcaaa tattcaatca catcaccgta agactctgta
     2221 tacaaattgc tggtagtcgc ggcaatagtg gtattggcaa aagtttggcc ttgattttca
     2281 accatcgcca acaattgatt tttaaaaaat ggcaataaaa tcaacagtag cacaatggca
     2341 aaactggcaa tcgcgatggc tgctcgtgta aagaaaaagc caaaaatacc aaaaaaccat
     2401 gacttaatca ttttaatttg gccttgtctt ggcgcgtatc cgccaccaaa tcctgcacac
     2461 ttttcaggtc ttcaaggctt actttttgca tggtgccata tttgtagttt ttcatcagcg
     2521 ttaagcgatg aatcgccc
//
LOCUS       DAOQSI010000214         2522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_49695_length_2522_cov_4.593028, whole genome shotgun sequence.
ACCESSION   DAOQSI010000214 DAOQSI010000000
VERSION     DAOQSI010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49695_length_2522_cov_4.593028"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..388
                     /gene="rpsR"
                     /locus_tag="PKL42_10000"
     CDS             155..388
                     /gene="rpsR"
                     /locus_tag="PKL42_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNU67446.1"
                     /translation="MARDMYKRRRYCRFSAEGIKEVDYKDVDLLKDFISENYKIIPAR
                     MTGTKARYQRQLTTAVKLARFLALLPYTDKHPG"
     gene            400..849
                     /gene="rplI"
                     /locus_tag="PKL42_10005"
     CDS             400..849
                     /gene="rplI"
                     /locus_tag="PKL42_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNU67447.1"
                     /translation="MQVILLEKVGNLGNLGDIVKVKDGFGRNFLIPQGKAKRATEANK
                     AEIAARRAELEKQQAAILVAAQARGEKLAGFVITVAQKAGVDGRLFGSVTNGDIADAL
                     SAQGHEVAKSLVRLPNGPLKTIGDHPVSVALHHDVVVDITVTVVAEA"
     gene            1140..2522
                     /gene="dnaB"
                     /locus_tag="PKL42_10010"
     CDS             1140..2522
                     /gene="dnaB"
                     /locus_tag="PKL42_10010"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNU67448.1"
                     /translation="MADEQLDALKIPPHSVEAEQSVIGGLLLENEALDKIADILSPND
                     FYRHDHKLIFQHIGKLIEHNRPADIVTVAESLESSAELSGVGGIAYLGALAQNTPTAA
                     NIRRYAEIVHERAVMRQLVTVGSGIAESAYAPNGRDAQQLLDEAEAKIFQIAEGGKRS
                     TQGFQDIKLLLPQVADRIDYLYSRDNPNDVTGIPTGFADLDSMTSGFQPGDLIIVAGR
                     PSMGKTAFSLNIAENVALDTKLPVAVFSMEMASTQLAMRMIGSVGRLDQHRMRNGNLE
                     DEDWEKLTTALGKLNEAPIFIDEGAGLSSFDVRARARRLHRQTGKLGLIVVDYLQLMA
                     APAGRQGENRATEISEISRSLKALAKELDVPVVALSQLNRSVEQRPDKRPVMSDLRES
                     GAIEQDADVILFIYRDEVYNPDSPDKGTAEIIIGKQRNGPIGRVRLTFIGQHTRFENF
                     AGTSYMADEY"
BASE COUNT          727 a          513 c          619 g          663 t
ORIGIN      
        1 gcgattggcg acttagcttt aacaaagctg gcattaggca cacaaatccg cgtaactggt
       61 tttttagcca aacgcagcct taaaagcacg caattagtga tgcacattaa ccagatagcg
      121 gttttaaaca attgaaagtt taggagtaaa taacatggca agagatatgt acaaacgccg
      181 ccgttactgc cgtttttcag cagagggcat caaagaagtt gattacaaag atgtagattt
      241 attgaaagat tttatttcag aaaactataa aattattcca gcgcgtatga cgggtacaaa
      301 agcacgttat caacgtcaat taacaactgc agtgaaactt gcacgtttct tagcgttgtt
      361 accatacact gataaacacc caggctaagg agaaacaata tgcaagtaat tttgttagaa
      421 aaagtaggca accttggcaa tttgggcgac atcgttaaag ttaaagatgg ttttggccgc
      481 aactttttaa ttccacaagg taaagcaaaa cgcgcgacag aagccaacaa agctgaaatc
      541 gctgctcgtc gtgcagagtt agaaaaacaa caggccgcta ttttagtggc tgcgcaagcg
      601 cgcggtgaaa aacttgctgg ttttgtgatc acagtcgctc aaaaagccgg tgttgatggc
      661 cgcttgtttg gctcagtcac taatggtgat attgcagatg ctttatctgc acaaggccat
      721 gaagtagcta aatcattggt gcgtttgcca aatggcccat tgaaaaccat tggcgaccat
      781 cctgttagcg ttgcattgca ccatgatgtt gttgtagata tcacagtaac cgttgttgct
      841 gaggcataat cgctttaaaa ttagcgtgat ataaaacgct aacaacacaa taaaaaaggc
      901 tactgaatgt agcctttttt attgcctaaa attccccgag agaaaaatat gccgctggaa
      961 gccttgtaaa taaaggctcg cagggcatcg aaaaaggtta ggtgtaattt aaggcaaaaa
     1021 atagcatttg tatgcattgt taaagcgtta gtttttggcg aattatttgg cattgcaatg
     1081 tcgtactaaa ttaccaaaat tgacagcaga agcgattaac gttcggtata atctcaagca
     1141 tggctgatga acaattagat gcattaaaaa tcccacccca ttctgtagaa gcagagcaat
     1201 cggtcatcgg tggcttgcta ctcgaaaacg aagcgcttga taaaattgct gatattttat
     1261 cgcccaacga cttttatcgg cacgaccaca agttgatttt tcagcacatt ggcaagttga
     1321 tagagcataa tcgtcctgca gatattgtga cggtggctga gtcactggag agttctgcgg
     1381 agctttcagg tgttggcggt atcgcttatt tgggcgcgtt ggcacaaaat acgccaacgg
     1441 cggcgaacat acgccgatat gcagagattg tgcatgaacg tgccgtgatg cgacagctag
     1501 tcacagtcgg ttcaggcata gcagaaagcg cttacgcgcc caatggacgt gatgcgcagc
     1561 agttgctcga tgaagcagaa gccaaaattt ttcagattgc tgaaggcggc aagcgtagta
     1621 cccaaggctt tcaggatatt aaactgttgc ttccgcaagt ggctgatcgc attgattatt
     1681 tgtactcaag agataatcct aatgacgtaa ctggtattcc taccggattt gctgacttag
     1741 attcgatgac ctctggtttt cagcctggcg atttaattat tgtggcgggt cggccatcga
     1801 tgggtaaaac ggctttctcg ctcaatattg ctgaaaatgt tgcactagat accaagttac
     1861 ctgttgccgt gttttcaatg gagatggcgt ccacacagct tgcgatgcgg atgattggtt
     1921 cggtagggcg cctagatcaa catcgcatgc ggaacggcaa tcttgaagat gaagactggg
     1981 aaaagctcac caccgccctt ggcaaactga acgaagcgcc catttttatt gatgaaggcg
     2041 caggcttaag tagctttgac gtgcgagcga gggcgcgcag gctgcatcgc caaacaggta
     2101 agctcggttt gattgtggtt gattacttgc agttgatggc cgcacctgcc ggcagacaag
     2161 gtgagaaccg cgccacggaa atttccgaaa tttcacgttc gctcaaggcg ttagcaaaag
     2221 agcttgatgt gccggtagtg gcactttcac aacttaatcg ttccgttgag cagcgccctg
     2281 ataaacgccc agtgatgagt gacttgcgtg aatctggtgc gattgagcaa gatgccgacg
     2341 tgattttatt tatttatcgt gatgaagtgt ataaccccga tagcccggat aaaggcacgg
     2401 ctgaaattat tattggtaaa caacgtaatg gcccgatagg tcgcgtgcgc ctcacattta
     2461 ttggacaaca tacgcggttt gaaaattttg ccggtacaag ttatatggcg gatgagtact
     2521 aa
//
LOCUS       DAOQSI010000215         2515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_49923_length_2515_cov_4.839431, whole genome shotgun sequence.
ACCESSION   DAOQSI010000215 DAOQSI010000000
VERSION     DAOQSI010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49923_length_2515_cov_4.839431"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PKL42_10015"
     CDS             <1..420
                     /locus_tag="PKL42_10015"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNU67449.1"
                     /translation="PGAVVVVPILPNGNVILEKQFRYPLHQVFIELPAGKIDANETVL
                     TTGQRELLEETGYTASEWVRLGCQHPCIGYSNEVIHMYLAQGLTAGQHRRDEDEMLEV
                     FNVPFDECISMILSGEITDGKSIVALYFAEKYLANTA"
     gene            475..1698
                     /locus_tag="PKL42_10020"
     CDS             475..1698
                     /locus_tag="PKL42_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HNU67450.1"
                     /translation="MLHLTRKLIESCIKLLLAISLTLYSVNASAEDRNAVSNDWLVAF
                     KNEARVNQISDKTIEATLQHAKFLPRVIVLDRSQPEFISPFLTYLDTRVNAQQISQGR
                     QMLQLNEALFVKVEAQYGVPKEVLVAFWGMETNYGKNKGSFGLPSALMTLAYEGRRSD
                     FFRSQLLDVMRIVDAGHNHVSAMRGSWAGAMGHMQFMPSTFMLYGVDADQDGRINIWT
                     SLEDAFASAANYLSQVGWRKNEISALEVKLPQGFQYQLAQLNMRKTSDEWAQLGVVAI
                     DNSQLPKQDNAAILLPQGWHGPAFMVFSNFDAIMDWNKSVNYALSVNHLANQLKDDLP
                     VVGGVEAEKAALSYNQLWALQTKLNNLGFDCGEPDGFPGLKTQAAIRSYQATQELPQD
                     GFAGPSLYNRLMKQH"
     gene            complement(1717..2274)
                     /locus_tag="PKL42_10025"
     CDS             complement(1717..2274)
                     /locus_tag="PKL42_10025"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HNU67451.1"
                     /translation="MPNDFVFQLAQRAKQIQKTGALRVANVGKHMQLNDAIRSDSTYW
                     LDDNSPNVAEQNYFAIMNTVKSQVNRALYLGLDTLESHFAIYSIGSFYKTHLDQFKTD
                     IYKNDDLRKLSCIIYLNEDWRPDDGGELRFYSDFERGQFIDICPQGGTLVAFLSEQFY
                     HEVLPAKRERISLTGWFKTRANSLL"
BASE COUNT          713 a          500 c          585 g          717 t
ORIGIN      
        1 ccaggtgcag tagtggtggt gccaatttta cccaatggca acgtcatact tgaaaaacaa
       61 tttcgttacc cgcttcatca agtatttatt gagctacccg caggtaaaat agatgcaaat
      121 gagaccgttc tgacgactgg tcaacgtgaa ttgctggaag aaaccggcta cacagcaagt
      181 gaatgggttc gcttaggttg ccaacatcca tgcattggct attcaaacga agtgattcat
      241 atgtatttgg cgcaaggatt aacggcaggt caacatcgtc gagatgagga tgaaatgcta
      301 gaagtattta atgtgccttt tgatgaatgt atttcgatga ttcttagcgg tgaaatcaca
      361 gatggaaaat cgattgtggc gctctatttt gcagaaaaat atttggcgaa tacggcttaa
      421 ttttcggtag accataacgc gtcatttatt tacttaatat tcattacagg cagtatgctt
      481 catttaaccc gcaaacttat cgaatcttgc atcaaactct tgcttgccat ttcactgaca
      541 ctctatagtg ttaatgcatc tgctgaagat cgtaacgctg tatccaatga ttggctggtt
      601 gcgtttaaaa atgaagcgcg cgtgaatcaa atttcagata aaacgataga ggccacgctt
      661 cagcatgcaa aatttttgcc gagggtgatt gtgcttgatc gttctcagcc agagtttatc
      721 tcaccatttt tgacttacct tgatacgcgt gtgaatgcac agcaaatatc ccaaggcaga
      781 cagatgctgc aactcaatga agcattattc gtaaaagttg aggcgcagta tggcgtccct
      841 aaagaagtac tggtcgcgtt ttggggaatg gaaacaaatt acgggaaaaa taaaggtagc
      901 tttggtcttc cttccgccct tatgacgctt gcctatgaag gaagacggag tgattttttt
      961 agatcacagt tgctagatgt aatgcgtatt gttgacgctg gacataatca cgtttcggcc
     1021 atgcgcgggt catgggcggg cgccatgggg catatgcaat ttatgccatc cacgtttatg
     1081 ttgtatgggg tagatgctga ccaagatggc cgcatcaata tttggacttc gttggaggat
     1141 gcctttgctt cggccgccaa ttacctatct caggtgggat ggcgtaagaa tgaaatctcg
     1201 gcgttagagg ttaaattgcc gcagggcttt cagtatcaat tagcacagct taatatgcga
     1261 aaaacatctg atgaatgggc gcaactcggc gttgttgcta ttgataatag ccaactacct
     1321 aagcaagaca atgccgcaat tttgctgcct caaggatggc acggccctgc gtttatggtg
     1381 tttagtaatt ttgatgcaat catggattgg aataagtcgg tcaattacgc actttcagtg
     1441 aatcatttgg cgaaccaact aaaagatgat ttacctgttg tgggtggtgt ggaggctgaa
     1501 aaagcggcac tgagctacaa tcaactttgg gcgctacaaa ccaaattaaa taatttggga
     1561 tttgattgtg gcgaaccgga tggttttcct ggactgaaaa cgcaggcggc gattaggtcc
     1621 tatcaagcaa cgcaagagtt accgcaagat gggtttgctg gccccagttt atacaatcga
     1681 ctcatgaaac agcattgaga cacactcggt ttaaaattac aataaagaat tagcgcgcgt
     1741 tttaaaccag cctgtaaggc tgatgcgctc acgtttagct ggcagcactt cgtgataaaa
     1801 ctgctcggac aaaaaagcga ctaacgtacc accttgagga cagatgtcaa taaactgtcc
     1861 gcgttcgaaa tctgaataaa atctcagttc accaccgtcg tctggacgcc aatcttcatt
     1921 taaataaata atgcagctca gtttacgtaa gtcatcgttt ttgtaaatat cggttttaaa
     1981 ttgatccaaa tgcgttttgt aaaagctacc tattgaatat attgcaaaat gactttcgag
     2041 cgtatcaagc ccaagataga gtgctcggtt aacttggctt ttcaccgtat tcatgatggc
     2101 aaagtaattc tgttcagcta cgtttgggct attatcatca agccaataag tactgtcgct
     2161 ccgtatggcg tcattcaact gcatgtgttt gccgacattg gctacgcgca gggcaccagt
     2221 cttttgaatt tgtttggccc gttgggctaa ttgaaacaca aaatcatttg gcataaaatt
     2281 tggcagtatt gcgtaacctt gcaaagctag ctggtcgacg agaaattcga attgttctaa
     2341 aggcacgcga tgcgacatat tttttctcag taaaaccaaa ttgtaacgta tataaaagct
     2401 catcgaatag gattctgttt gcttagagaa agcgaatgcg ctagaattcg gcctcgttca
     2461 aaaataggcg gttagctcag ttggttagag cgcttggtcg acatccaaga ggtca
//
LOCUS       DAOQSI010000216         2463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_51616_length_2463_cov_4.221761, whole genome shotgun sequence.
ACCESSION   DAOQSI010000216 DAOQSI010000000
VERSION     DAOQSI010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51616_length_2463_cov_4.221761"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /gene="bamA"
                     /locus_tag="PKL42_10030"
     CDS             <1..963
                     /gene="bamA"
                     /locus_tag="PKL42_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNU67452.1"
                     /translation="NQNNFLGTGNRVSAQVNTGKVNTTYSLSFTDPYFTPDGVSRGFD
                     IYRRDVDTGSLSVGSYSSSSYGGGVRFGIPLNERDGFNFGLAADFTKVDLNDQSPIQY
                     QKFCGNTSGCSSNSIVASAGWYHDTRDSIMFTNNGVLQKLSGEFSLPVLDLNYYKIDY
                     KHAWFKNVYKDFTFMLNGEIGYADNYGSKNYPFFKNFFMGGVNSVRGYDNGSLGPRDL
                     DPTTNSDFAVGGTKRLLGNAELFFPVPGLKDSKQFRMSAFVDGGNVWGSESGYSLGDL
                     RYSTGLGVSWLSPFGPLKLIYARALNSEKGDDTQNVQFQLGQQF"
     gene            1070..1534
                     /locus_tag="PKL42_10035"
     CDS             1070..1534
                     /locus_tag="PKL42_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNU67453.1"
                     /translation="MVVSFGLQAADLKVGYVQVDKILQEAPQTAESGKKLEKEFGPRS
                     QELDRLAKQIKELETVLEKEGVTIPETERRTKERDVQNIKVEFQRKQRELREDINLRK
                     NEELGSLQDRINKAVQSVAKSENYDLVMYSGVAYAADKIDITDKVLKLLGKK"
     gene            1567..>2463
                     /gene="lpxD"
                     /locus_tag="PKL42_10040"
     CDS             1567..>2463
                     /gene="lpxD"
                     /locus_tag="PKL42_10040"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNU67454.1"
                     /translation="MTQQYSLRDISSRFGGQVSGDENIRISRVSSLGNAKQGDICFIN
                     DAKYQKALADCKASAFILRENDAALTSQPKIIVDNPYAYFAKISSLLNPPAISHIGID
                     PSAMVSSSATVPSSCSIGPMVVIGEQVVLGENVIIGAGCVLENDVVIGDNTRLEPNVT
                     IKHHCQLGAHCHIFSGAVIGSDGFGYAEEAGVWLKIPQVGRVIIQDNVDVGANTTIDR
                     GALDDTIIEEGVKLDNLIQIGHNCVIGAHTVIAGCTGIAGSAKIGRHCKIGGAAMILG
                     HLVIVDGVTVSPGSMITRSLMQA"
BASE COUNT          718 a          438 c          587 g          720 t
ORIGIN      
        1 aaccaaaata atttcttggg cactggtaat cgcgtaagcg cgcaggtgaa caccggtaaa
       61 gtgaatacta catattcact atcatttact gatccttact ttacgccaga tggcgtgagt
      121 cgtggttttg atatctatcg gcgtgacgtt gacactggtt cattgagcgt aggttcttat
      181 agttcatcct cttacggtgg cggggtgcgc tttggtattc ctctgaacga gcgggatggc
      241 tttaactttg gtttggcggc agatttcaca aaagtcgatt tgaatgatca aagtccaatc
      301 cagtatcaaa aattctgcgg taacacttca ggctgttcaa gtaactctat agttgcttct
      361 gctggttggt accatgacac gcgtgacagc attatgttta ccaataatgg cgtattgcag
      421 aaactcagtg gagagttttc tctacctgta ctcgatttaa attactacaa aattgactat
      481 aagcatgcat ggtttaaaaa tgtatacaaa gattttacat ttatgctcaa tggcgaaatt
      541 ggttatgcgg ataattacgg cagtaaaaac taccccttct ttaaaaactt ttttatgggt
      601 ggcgtaaact cagtccgtgg ttatgacaat ggatcattag gacccagaga tttagacccc
      661 acaactaatt ctgattttgc ggtcggcgga acgaagcgtt tgcttggtaa tgccgagttg
      721 tttttcccag ttccaggctt gaaagactct aaacaattca gaatgagtgc tttcgtggat
      781 ggtggtaatg tttggggttc tgagtcaggt tatagcttgg gtgatttgcg ctactcaacc
      841 ggcttaggcg ttagttggtt gtcaccattt gggccgttaa aacttattta tgcaagagca
      901 ttgaacagcg aaaaaggcga tgatacgcaa aatgtccaat ttcagcttgg tcagcagttt
      961 taaatcatca ttcacatatt gctaaatcat gactgattga attaggagat ttaaattgag
     1021 ttatcagatt ttaaaattca caaaacgcaa attagcgctt ttgagcttaa tggtagtttc
     1081 atttggcttg caagctgctg acttaaaggt cggttatgtg caagttgata agatcttgca
     1141 agaagcaccg caaactgccg aaagcggcaa gaaacttgaa aaagagtttg gcccgcgctc
     1201 acaagagctg gatcgcttag caaaacaaat caaagagctt gaaacggtcc ttgaaaaaga
     1261 gggcgttacc attcctgaaa ccgaacgccg tactaaggag cgtgatgttc aaaacatcaa
     1321 agtggaattt caacgcaagc aaagagaatt aagagaagat atcaacttac gtaaaaatga
     1381 agaactaggc agtctgcaag accgcattaa taaagctgta caatctgtgg caaagtctga
     1441 aaattatgat ttggtcatgt acagcggtgt tgcctatgcg gcagataaaa ttgatatcac
     1501 ggataaggta ttgaaattat taggtaaaaa ataaagtttt aaaattaatg ttaatgtgac
     1561 tatcgaatga ctcaacaata ttcattgcgt gatatttcat caagatttgg tggtcaagtg
     1621 agcggggatg agaatatacg catttctcgt gtgtcatcac tcggtaacgc taaacaaggt
     1681 gacatttgtt ttattaacga tgccaaatat caaaaagcac ttgcagattg taaagccagt
     1741 gcttttattt tgcgcgaaaa tgacgcggca ctaacatcac agccaaaaat aattgtagat
     1801 aatccctatg cttattttgc taaaatttct tcgcttttaa atccacctgc tatttcacat
     1861 atcggtattg atccaagcgc tatggtgagt agtagtgcaa cggtaccaag ctcttgcagt
     1921 attgggccaa tggtggtgat tggtgagcaa gtcgtccttg gtgaaaatgt tattattggt
     1981 gcaggttgtg tgcttgaaaa tgatgttgtg attggtgata atacaaggct agaacccaat
     2041 gtcacgatca agcatcattg ccaactgggt gcgcattgtc atattttttc aggtgcagtt
     2101 attgggtcgg atgggtttgg ttacgctgaa gaggctggtg tatggctcaa gattccgcag
     2161 gtggggcgtg tcatcataca agataatgta gatgttggtg cgaatacgac gatagatcga
     2221 ggcgcgttag atgacacgat catagaagaa ggtgtgaaac tcgacaatct cattcagatt
     2281 gggcataact gtgtgatagg agcgcataca gtcatcgctg gctgcacggg tattgccggc
     2341 agtgcgaaaa ttggcaggca ttgtaaaata ggtggcgctg ccatgatttt aggccactta
     2401 gtaattgtgg atggagtcac cgtgtcaccc ggcagtatga ttacacgctc tttaatgcag
     2461 gcg
//
LOCUS       DAOQSI010000217         2418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_53147_length_2418_cov_7.797715, whole genome shotgun sequence.
ACCESSION   DAOQSI010000217 DAOQSI010000000
VERSION     DAOQSI010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53147_length_2418_cov_7.797715"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PKL42_10045"
     CDS             <1..198
                     /locus_tag="PKL42_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNU67455.1"
                     /translation="LMCDIAKQIDKHQWTQSEAAKRFGVSQARVSDLVRGKFDKFSLD
                     TLIQLATRAGHSVRIELAEAA"
     gene            269..547
                     /locus_tag="PKL42_10050"
     CDS             269..547
                     /locus_tag="PKL42_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNU67456.1"
                     /translation="MSKIEDPEMATFERDLLLSVEQMKAGKVRVALSPIANIRNKMHL
                     SQSKFSDLLGVSVRTLQDWEQGRAKPSGAAKALLKIAELNPEALIAVA"
     gene            complement(584..865)
                     /locus_tag="PKL42_10055"
     CDS             complement(584..865)
                     /locus_tag="PKL42_10055"
                     /inference="COORDINATES: protein motif:HMM:NF033894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HNU67457.1"
                     /translation="MKLTLITLMAVLLTACSGQDKTDSQKTYTVQELLKEPALYKKTS
                     DWCSENPAERSLTPNCMNALQALHELQANATLEDRGKPTSAPQPIANFN"
     gene            complement(894..1598)
                     /locus_tag="PKL42_10060"
     CDS             complement(894..1598)
                     /locus_tag="PKL42_10060"
                     /inference="COORDINATES: protein motif:HMM:NF019608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HNU67458.1"
                     /translation="MKKHHLKPHVKAFIIALTLAASLQASITPANAGGIPVIDVANLA
                     NTLENIVQWGKQLQAMKEQYAQQLAQYQSMTGKRGFGTVLNDPNLRQYLPADAQKVYS
                     QMMRPVQSMDVCYGLTGESLTLCQAEVKKHYIDRDNYEKAYDTASTEQTQIQGLIDQI
                     SNTDDPKAIAELQARMAGEQAKIQNTVVQMQLSAQLAEIQNKLLQQTKQDAQVKATIE
                     DRGNPISAPEPITNWD"
     gene            complement(1652..2239)
                     /locus_tag="PKL42_10065"
     CDS             complement(1652..2239)
                     /locus_tag="PKL42_10065"
                     /inference="COORDINATES: protein motif:HMM:NF019069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="HNU67459.1"
                     /translation="MNINQHLKLSVILLSTLVMSIAHADSGELTGKVKLACEAILCLS
                     TGSPPGECSPSLSEYFSIDFDDFSDTIQGRINFLNLCPVSSETPQMKSLVNAIANGAG
                     RCDAASLNATLITYTNGLQGCIQGTKPTYCSAYEGHAYTDLHNGAHYVVTQSQTYWPF
                     NNFMGSNSNNSGSFLNGFTGGNGQNSTQTCGHWVD"
BASE COUNT          613 a          495 c          542 g          768 t
ORIGIN      
        1 ttgatgtgcg atatcgcaaa gcaaatagac aagcatcagt ggacacaaag cgaggctgca
       61 aaacgctttg gtgtatctca agcccgcgtt tctgacttgg tgcgcggtaa atttgataaa
      121 tttagtttgg atacgctgat acaacttgcc acgcgcgctg gacatagtgt acgtattgag
      181 ttggcagagg cggcttaatt ctgttgtgcg tttggtatta agcctcacgc catgatttaa
      241 ctgaattaat tgaacgaggg cacctaaaat gagtaaaatt gaagatcctg aaatggcgac
      301 atttgaaaga gatttattac tttctgttga gcaaatgaaa gctggtaaag tgcgcgtagc
      361 attgtcacca attgctaata tccgcaataa aatgcactta agccaaagca aattttctga
      421 tttattaggc gtgtctgtac gcaccttgca agattgggag caaggtcgcg ccaagccctc
      481 tggcgcagct aaagcgctac ttaaaatagc ggagcttaat ccagaagcac ttatagcggt
      541 cgcttgatta aaataagatt tcatttttta tctaaaattg tgcttagtta aaattagcta
      601 ttggttgagg tgcagaggta ggttttcctc tatcctctag cgtggcgttt gcttgtaatt
      661 catgtaaagc ctgcaaggca ttcatgcagt tgggtgttag gctgcgctca gcaggatttt
      721 cactgcacca atcacttgtc tttttataga gtgctggctc tttcaataac tcctgcactg
      781 tgtaagtctt ttgcgagtca gttttgtctt gcccagaaca cgccgttaat aaaactgcca
      841 taagcgtaat aagtgttagt ttcatgatgc aaattccctt attgatagct tggttaatcc
      901 caattggtaa tcggttctgg cgcagaaata ggattgcctc tatcctcaat cgtggctttt
      961 acttgcgcat cttgtttagt ctgttgtaat aacttgtttt gtatttcagc aagctgtgcg
     1021 ctcagctgca tttgcaccac ggtgttttga atcttggctt gttcgcctgc catgcgggct
     1081 tgcaattcag caatggcttt agggtcatcg gtattgctaa tctgatcaat taaaccttgt
     1141 atctgtgttt gctctgtact ggcggtgtcg taagcttttt cgtaattgtc tcggtctatg
     1201 tagtgttttt taacttctgc ttggcacagg gtaagagact cacccgttag cccgtaacaa
     1261 acatccatgg actgtactgg tcgcatcatt tggctatata ctttttgtgc gtcagctggt
     1321 aaatattggc gtaaattagg gtcgtttaat accgtgccaa agccacgttt ccccgtcatt
     1381 gactgatatt gggcaagttg ttgtgcgtat tgctctttca tggcttgtag ctgcttaccc
     1441 cattgcacaa tattctcgag cgtattagca agattcgcca catcaataac aggaatgccg
     1501 cctgcattgg caggcgtaat actggcttgt aagctggccg caagggttag cgcaataatg
     1561 aatgctttaa catgcggttt taagtgatgt tttttcatga tatttatcct tttatgtcct
     1621 atatcggttg ttttatatat caacaattaa atcaatcaac ccaatgacca caagtttggg
     1681 tgctgttttg gccgtttcca ccagtgaagc catttagaaa actcccgcta ttgtttgaat
     1741 tactacccat gaagttatta aatggccaat acgtctgtga ctgcgtcacc acgtaatgcg
     1801 caccattgtg taagtcggta tatgcatggc cttcataggc tgaacagtag gtaggtttag
     1861 tgccttgaat acagccttgc aacccattgg tataagtgat tagcgtcgca ttcagtgagg
     1921 ctgcatcaca acgccctgcg ccattggcaa tcgcattgac caagcttttc atttgcggtg
     1981 tttctgatga aactggacat aggtttaaaa agttgatgcg accttgtatg gtgtcgctga
     2041 aatcatcaaa atcaatactg aaatattcac tcagtgatgg tgaacattcg cctggcggcg
     2101 aacctgtgga aaggcaaaga atagcttcgc aagctaattt aactttgcct gtgagttcgc
     2161 cgctatccgc gtgcgcgata ctcatcacca aagtgcttaa caggatgaca gataatttta
     2221 gatgttggtt tatgttcacg atgatgtcct taactgatca aaaacttatt gggatactcg
     2281 cccttattgg ctttagtcat ggctttacgc tgggctaatc gatttaataa aatgggcatc
     2341 cagtcttgta cgtcatcacc cacttctgcg cgaatttcat cgagtatttc cacgttatct
     2401 aatgaagtac tgagtacg
//
LOCUS       DAOQSI010000218         2364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_55062_length_2364_cov_5.180598, whole genome shotgun sequence.
ACCESSION   DAOQSI010000218 DAOQSI010000000
VERSION     DAOQSI010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55062_length_2364_cov_5.180598"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..2013)
                     /locus_tag="PKL42_10070"
     CDS             complement(94..2013)
                     /locus_tag="PKL42_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNU67460.1"
                     /translation="MIQFKQLTLARGVKVLIQSASLQLHPGHKVGLTGANGAGKSSLF
                     ALLRGEMHLESGHLEMPASWVVAHVAQETPALAMPAIEFVLDGDVELRTIEKALAEAE
                     ANHQGELIGELHHRLTDIDGYSAKARAAELLNGLGFSQANLQQAVSTFSGGWRVRLNL
                     ARALMCRSDLLLLDEPTNHLDLDAVIWLESWLQSYRGTLFLISHDRDFLDAIVNHIAH
                     IEQQTLTLYKGGYSDFERQRAEKLALQQSMFEKQQRKVAHLHSYIDRFRVQATKARQA
                     QSRIKALERMEMISAAHVDSQFSFEFRAPLASPDPLLVLDEMSVGYNNVALIKNIVLA
                     IRPGERLGLLGRNGAGKTTLIKLLANELKPQLFDGKLGKRVEGKDLNIGYFAQHQLEQ
                     LRPDESPLQHMMKLDPLTREQEHLNYLGGFDFKGDMARSPCAHFSGGEKSRLALALLI
                     WTRPNLLLLDEPTNHLDLEMRHALTLALQDYEGGVILVSHDRALLRATCDEFILVADG
                     SAEPFESDLEGYSAWLNEQRLKEKQATQLIVENKPNKNDRAANKIERQARIAARRPLL
                     KEIEQLDNNIAQWHIDKTKCDERLNDSALYAANDKTELQQLLKKQSELSSQIETAEMR
                     WLELHELLEAIPALD"
BASE COUNT          637 a          586 c          474 g          667 t
ORIGIN      
        1 cacagcgcat gcatcagttt gagtcgtttt gaatattgaa tttccatttc aaatcctaat
       61 cattgtttaa aaatttacgc attttcatgc agattaatct agcgcaggaa ttgcctctaa
      121 caactcgtgc aattctagcc agcgcatttc cgcagtttca atctggctgc ttaattcaga
      181 ctgcttttta agcagttgtt gcaattcggt tttatcgttt gccgcataca gcgcactatc
      241 attcaaacgt tcatcgcact tagttttgtc aatgtgccac tgggcaatat tgttatctag
      301 ctgttcgatt tcttttaaca atggcctgcg cgctgcaatt ctggcttggc gttcaatttt
      361 attggcagcg cggtcatttt tattgggttt attctcaaca attaactgtg tcgcctgttt
      421 ttcttttaaa cgttgctcat tcaaccacgc actataacct tctaaatcgc tttcaaacgg
      481 ctcggcactg ccatcggcca ctaaaataaa ttcatcgcaa gtggcgcgca ataacgctcg
      541 gtcatgcgag actaatataa ctccaccttc gtaatcttgc agggcaagcg tcaacgcatg
      601 gcgcatttct aaatctaaat ggttggtggg ttcatcgagt aacagcaaat ttggccgcgt
      661 ccatattaaa agtgccaatg ccaagcgtga tttttcccca cccgaaaaat gagcacatgg
      721 tgaacgtgcc atgtcacctt taaagtcaaa gccgcccaaa taattcaggt gttcttgctc
      781 gcgtgttagc gggtctagct tcatcatgtg ttgcaagggt gattcatctg gccgcaattg
      841 ctcaagctga tgttgtgcaa agtagccgat gtttaaatct ttgccttcaa cgcgtttacc
      901 caatttacca tcaaaaagtt gtggctttag ttcattcgca agcaatttaa tgagcgtggt
      961 tttacctgca ccattacgcc ccagcaatcc caatcgctcg cccggcctga tggccaacac
     1021 aatattctta atcaatgcca cattgttgta gccgactgac atttcatcta acaccagcaa
     1081 tgggtcggga gaagcgagtg gcgcacgaaa ctcaaaacta aattgcgaat ccacatgcgc
     1141 ggctgaaatc atctccatgc gctcaagcgc tttaatgcgg ctttgtgctt ggcgtgcttt
     1201 ggtggcctgc acgcgaaaac ggtcaatata actgtgtaaa tgcgccactt tgcgttgctg
     1261 cttttcaaac atgctctgct gcagagccag tttttcagca cgttggcgct caaaatccga
     1321 atagccacct ttatacaaag ttagcgtttg ctgctcaata tgcgcaatat gattcacaat
     1381 cgcatctaaa aagtcgcggt catgcgaaat caaaaacagc gtaccgcgat aactttgcag
     1441 ccagctctca agccaaatca ccgcatctaa atctaaatga ttggtgggct catctaacag
     1501 caataaatct gagcgacaca tcaaagcgcg tgccaagttt aaacgcacac gccaaccgcc
     1561 agaaaaagtt gaaactgctt gctgcaagtt agcttggcta aagcctaagc cgttcaataa
     1621 ctccgccgca cgcgctttgg cactatagcc atcaatatcc gttaagcgat gatgcagttc
     1681 acctatcaac tcgccttgat gattggcttc tgcttcagcc aaggctttct caattgtgcg
     1741 caactcaaca tcgccatcta acacaaattc aatcgcaggc atggcaagtg cgggggtttc
     1801 ttgcgccaca tgcgccacca cccagcttgc gggcatttct aaatgacccg attccaaatg
     1861 catttcacca cgcaacagcg caaacaatga cgatttacca gcaccatttg ccccagttaa
     1921 gcccacttta tggccaggat gcagttgcag agatgcggat tgaatcagga ctttgacgcc
     1981 gcgggcgagt gtgagttgtt taaattgaat caattgaaaa ccgttaattt taaaagtcta
     2041 gtcaaccaag ttcgaccagt tgcgctattc taatagataa cgaatcgaat tttgacaact
     2101 ttttatgaca ctagcgtttg gtgtaacgcc agttttaacc aatattgcag taaaataaca
     2161 gtctgccgcc atccacttca cagcatagaa taaacaaacc taccgaatgg ttacaaaaac
     2221 actacgactt ttctaccgcg ttattttaaa actcattacg tttgtcgtgg ttgtgatggt
     2281 gcttgcagcg ctggttattc agttctatgt attccccaac attgaccgct acaaaaacga
     2341 tattgctaaa cttgccagtg aggc
//
LOCUS       DAOQSI010000219         2363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_55101_length_2363_cov_4.608319, whole genome shotgun sequence.
ACCESSION   DAOQSI010000219 DAOQSI010000000
VERSION     DAOQSI010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55101_length_2363_cov_4.608319"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="PKL42_10075"
     CDS             complement(<1..720)
                     /locus_tag="PKL42_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HNU67461.1"
                     /translation="MADSAFSYSIDEIKQMSQDVLKVAKQMGASAAEAELSLSIGQNV
                     SVRLGETENIEYNRDKGMSVTVYFGQQKGHASTSDLNQQAIYDTVAAACNIAKYTAQD
                     AYCGLAEAGLMAKESLDLDLYHPWAISVEEAIAIAKTCEASARAVDARITNSEGASVS
                     TGESCFAYSNSHGFTGGYLSSRHGVSCSVIAEADDNMQRDYWYSTARAASDLDAAIAV
                     GHKAGERTVRRLNSRKIKTCQV"
     gene            833..1360
                     /gene="yjgA"
                     /locus_tag="PKL42_10080"
     CDS             833..1360
                     /gene="yjgA"
                     /locus_tag="PKL42_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HNU67462.1"
                     /translation="MIASDEPLPISKTKLKAEADAQQALGVRLCELPKDKLAKLDLPE
                     ALYAAVLDSKKITANGAIRRHRQYLGRLMRDIDTAPIEEQLARWDGKHTAENAYFHGL
                     ERWRDRMIADANVLQEFLALYPGTDIQQLRTLIRNAQKELSANKPPKSSREIFKLLRE
                     ITQPQQTEATQETDR"
     gene            complement(1457..2245)
                     /locus_tag="PKL42_10085"
     CDS             complement(1457..2245)
                     /locus_tag="PKL42_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNU67463.1"
                     /translation="MTVTVSKKLTKLKTNLMLLSLLSMGTLIFTQQLFAADNPYQENY
                     KAQNTNQLKSANANPDTKIEIKNQKITDNSIDVDNVRMLEDGYDMMGWSGFSANEAPA
                     DLALSHGRAIQADQVILYSKHEAEKNKSADVKSDVKSNPDEQDLHYFYASYWAKLPMP
                     LLGVHVVKLRKLLTNQQAATASANEELPEEPGLKVIAVIKSSPAAEANILRGDVLMKV
                     GDATVNQQAELVAAVKRYAGQSVPVVLIRNNKEIQVTAKLNSRT"
BASE COUNT          642 a          581 c          463 g          677 t
ORIGIN      
        1 cacttggcaa gtcttaattt tacgggaatt taaacgtcta acggtacgct cacctgcctt
       61 atgccctaca gcaatcgcgg catctaaatc actggctgca cgggcggtgc tgtaccaata
      121 atcgcgctgc atattgtcat cggcttctgc aatcaccgag cagctcaccc catggcgaga
      181 actcaaatag cccccagtaa aaccgtgact attactataa gcaaagcagc tttcaccggt
      241 tgaaacacta gccccttcag aattcgtaat ccgcgcatct actgcccttg ctgaagcctc
      301 gcacgttttg gcaatggcga tggcctcttc tacagaaata gcccatgggt ggtataaatc
      361 caaatccaga gactctttag ccattaaacc agcttctgcc aacccgcaat aagcatcttg
      421 cgcggtatat ttggcaatgt tgcacgcggc tgctactgtg tcgtaaatgg cctgctgatt
      481 taagtctgac gtgctggcat ggcctttttg ctgaccgaaa tagaccgtaa ctgacatacc
      541 tttatcacgg ttatattcga tattttccgt ttctcccaac cgtacagata cattttgacc
      601 aatacttaaa cttaactcag cttccgccgc gctagcaccc atttgctttg ccactttaag
      661 tacgtcttgt gacatttgct taatttcgtc gattgaataa ctaaatgctg aatctgccat
      721 aacgctttct actatctaaa tgttcgcttt aagactgcta taataccgca ctatgaaacc
      781 gcaaatatca gcgaaaccca aaaagaccgc caaagagact gaatcgactc aaatgattgc
      841 ttcagatgag ccgctgccca tcagcaaaac gaaattaaaa gcggaggcag atgcccagca
      901 ggcattaggc gttcgcctgt gcgaattgcc taaagacaag ctcgcaaagt tagacttgcc
      961 cgaagcgctc tatgccgccg ttctggatag caaaaagatt acggctaatg gcgctattcg
     1021 acgtcaccgt caatatttag gccgcctgat gcgtgatatc gataccgccc cgattgaaga
     1081 acaactggcg cgctgggatg gtaaacatac cgctgaaaac gcttactttc atggtttaga
     1141 gcgttggcgt gaccgcatga ttgctgatgc caatgtatta caagagtttt tagcgttgta
     1201 cccaggcaca gatattcagc aacttcgcac acttattcga aatgcgcaaa aggaactgtc
     1261 agcgaataaa ccgccaaaaa gtagccgaga aatatttaag ttattgcgtg aaataacgca
     1321 accgcaacaa actgaggcaa cgcaagaaac tgaccgttag ccgaacgctc gcttatcttg
     1381 ctatatcagg gcgtaccttg cgccctgata ttcgtcaaca gcatcgctaa agctttaaac
     1441 catgtgcttt cagccgctaa gtccttgaat taagctttgc agtcacttgt atttctttat
     1501 tgttacgaat taacaccact ggcacacttt ggcctgcata tcgcttaacc gcagccacta
     1561 attccgcttg ctggttcacc gtcgcatcgc ccactttcat cagcacatcg ccccttaaaa
     1621 tatttgcctc cgctgcgggg gatgacttaa tcacggcaat cacttttaaa cctggctctt
     1681 caggtaattc ttcattagct gaagccgtag ctgcttgctg gttggtgagc agttttctta
     1741 atttaaccac atgcacgccc agtaaaggca tgggtaattt agcccaatag ctcgcgtaaa
     1801 agtagtgtaa atcctgctca tctggattac tttttacatc gcttttaacg tcagcacttt
     1861 tatttttttc agcctcatgc ttgctataca aaatcacttg gtctgcttga atcgcacgac
     1921 catgactcag cgccaaatct gctggtgctt catttgcaga gaagccagac catcccatca
     1981 tgtcgtaacc atcttccagc atacgcacat tgtccacatc aattgagtta tcggtaatct
     2041 tttgattttt aatttctatt tttgtgtcag gatttgcatt ggccgatttt aactgattgg
     2101 tattttgcgc tttatagttt tcttggtaag ggttatctgc cgcaaaaagt tgttgggtaa
     2161 aaatcaatgt gcccatactc aacaaactga gtaacattaa attggttttt aatttcgtta
     2221 attttttgct caccgtcact gtcataaaaa atcctttttg ttgagtaatg ttatgatgga
     2281 atattcacac aacactcatg accagcaaat gacaaaagaa ttcattaaaa ttggattagt
     2341 ttctattagt gaccgtgcaa gta
//
LOCUS       DAOQSI010000220         2351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_55553_length_2351_cov_5.358885, whole genome shotgun sequence.
ACCESSION   DAOQSI010000220 DAOQSI010000000
VERSION     DAOQSI010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55553_length_2351_cov_5.358885"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="PKL42_10090"
     CDS             complement(<1..738)
                     /locus_tag="PKL42_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor protein PhoR"
                     /protein_id="HNU67464.1"
                     /translation="MLDIRWKAALTICALGIISLFLWLITSATTALIVFASGLIFYFI
                     RHLFWLSQLHDWLKKPALKTIPEGSGLWEDVFTGLLRYERSNVQQQNDLSAALERFQL
                     ATAAMPDGLVILSASNAIEWCTPHAESQLGLNLSQDKNLPIVNLIRNSHFIAYLYNEQ
                     FDEPFKLTPIHQPERSLEIELISLANQQKLLISRDMTQRDKVETMRRDFIANVSHELR
                     TPLTVVGGFLETLSDMEGAIPANLKTYF"
     gene            complement(961..1653)
                     /gene="phoB"
                     /locus_tag="PKL42_10095"
     CDS             complement(961..1653)
                     /gene="phoB"
                     /locus_tag="PKL42_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HNU67465.1"
                     /translation="MPANILVVEDEPAIQELLALNITQAGHNPIRALSVEIAQNLMRE
                     TIPDLILLDWMLPGMNGLEFARKLKSDNLTKTIPIIMLTARGDEYDKVRGLEVGADDY
                     VTKPFSPRELNARIKAVLRRRAPQMTDDPIEIDGLRLDPTSHRVTGHQKIVDLGPTEF
                     RLLHFFMTNAERVHTRSQLLDKVWGDRVFVEDRTVDVHIRRLRNALAVSGHQDLIQTV
                     RGSGYRLSEQHT"
     gene            1840..>2351
                     /locus_tag="PKL42_10100"
     CDS             1840..>2351
                     /locus_tag="PKL42_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNU67466.1"
                     /translation="MAGFLSKENTIANASFNRWLVPPAALAVHLSIGMAYGFSVFWKP
                     LGNALMDAEGKPLAACAAGATNFSEKLSGTLRALAATDCNWTQFDLGWMYTLFFVLLG
                     CSAALWGGWLEREGPRKAGLVSMMCWCGGLLISAYGVYTHQLWMMWLGSGVIGGIGLG
                     LGYISPVSTL"
BASE COUNT          593 a          495 c          593 g          670 t
ORIGIN      
        1 gaaatacgtt ttcaggttgg caggaatcgc gccttccata tcgcttaagg tttccaaaaa
       61 gccgcccacc acggttaatg gcgtgcgtaa ttcatgtgaa acgttggcaa taaaatcgcg
      121 ccgcatggtt tctactttgt cacgctgcgt catatcgcgg ctgatgagca gtttttgctg
      181 atttgccagt gaaatcagct cgatttcgag gctgcgctca ggttgatgga ttggcgttaa
      241 tttgaaaggc tcatcaaact gctcgttata caggtaagca ataaaatggc tgttgcgtat
      301 caaattcaca atcggcagat ttttatcttg gcttaaattc aggcccagct ggctttcagc
      361 gtgcggtgta caccactcaa tggcgttgct ggcgcttaat atgaccaaac catctggcat
      421 ggcggctgtg gccaattgaa aacgctctaa tgcagcactg agatcatttt gctgctgcac
      481 attactgcgc tcataacgta gcaggccggt aaatacatct tcccacaggc ctgaaccttc
      541 aggaatggtt ttgagtgctg gtttttttag ccaatcgtgc agttggctta gccaaaaaag
      601 atggcgaata aaataaaaaa taagcccgct ggcgaacacg ataagcgctg tggttgcgct
      661 agtaatcagc cacaaaaaca ggctgatgat accaagcgca caaatcgtta aggcagcttt
      721 ccagcgaata tcgagcacag aattggtttt tctaaaaacg aatgatggaa cgaattatag
      781 cgaggatttc acactaaatt tgtcaggctt aaaaatttaa ctaaatttag ccatttttaa
      841 tgccaacctt tttcgatgct ctgcgagcct tataaataaa ggctcgcaga gcataaattt
      901 tttctcggga gattgttgat gctaaattta gacaattttg cgcgtaaaaa acaatcaaaa
      961 tcaggtgtgt tgctctgata atcgatagcc agaaccgcgc acggtttgaa ttaaatcttg
     1021 atggccagac actgccaagg cattgcgcaa gcggcgaata tgcacgtcca ccgtgcggtc
     1081 ttcaacaaac acgcggtcgc cccagacctt atctaacaat tggctgcggg tgtgtacgcg
     1141 ctcggcattg gtcataaaga aatgcagtaa tctgaattca gtggggccaa gatcgacgat
     1201 tttttggtga ccagtcacgc ggtggctggt ggggtctagt cgtaagccat caatttcaat
     1261 cgggtcgtcc gtcatttgcg gggcgcggcg gcgcagcaca gcttttattc tggcattgag
     1321 ttcgcgtggg ctaaagggct tggtgacgta gtcatcagcg cctacttcta ggccgcgtac
     1381 tttgtcgtac tcatcgccac gcgcagtgag cataatgatg gggatggttt tggtgaggtt
     1441 atccgatttg agttttcgtg cgaattccag gccgttcatg ccaggcagca tccaatctaa
     1501 caaaattaaa tcaggaattg tctcgcgcat taaattttgc gcgatctcta cgcttaaggc
     1561 gcgaatgggg ttatgtccgg cctgcgtgat gttgagcgca agtaactctt ggatggctgg
     1621 ttcatcttca acaactaaaa tattggctgg catcaggttt ttcctcaaaa taacttgagc
     1681 gttagtttat gaccttaata tgacatattc gtgacaaata tcaagcaact tacaataaaa
     1741 gccgcacata agtaaattgc catttttcac aaattgttac acactgctac attataaaat
     1801 ttcataaaaa atggtagcgt aattttttga gggggcaaca tggcaggttt tttatctaaa
     1861 gaaaacacca tcgcaaatgc aagctttaat cgctggctgg tgccgccggc agcccttgct
     1921 gtgcatttat ctattgggat ggcgtatggc tttagtgtgt tttggaagcc acttggcaat
     1981 gcgctaatgg atgctgaggg taagccttta gccgcttgtg cggcaggggc aactaatttc
     2041 agtgagaagt tatctggtac tttgcgcgca cttgccgcaa ctgactgtaa ttggacgcag
     2101 tttgatttag gttggatgta tacgttattt tttgtgctgt taggttgttc tgcagcgcta
     2161 tggggtggct ggttagagcg cgaaggtccg cgtaaagcgg gtctggtttc aatgatgtgc
     2221 tggtgcggcg ggttgttaat ttcagcctat ggtgtttaca cgcaccagtt atggatgatg
     2281 tggctcggca gtggcgtcat tggcggcatt ggcttaggtc tgggttacat ttcccccgtt
     2341 tcaacgctga t
//
LOCUS       DAOQSI010000221         2289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_57952_length_2289_cov_4.876455, whole genome shotgun sequence.
ACCESSION   DAOQSI010000221 DAOQSI010000000
VERSION     DAOQSI010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57952_length_2289_cov_4.876455"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..1216)
                     /locus_tag="PKL42_10105"
     CDS             complement(8..1216)
                     /locus_tag="PKL42_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNU67467.1"
                     /translation="MFAKLSTQHLSISRYFRTIMLVVIAGMVVIGCTKKEPAEQASGN
                     MPPMPVSVLKLEAVSVPISAEAVAQTEGAKEVEIRPRVGGILLKKMFEEGAPVKAGQL
                     MFLIDPVPFQIALANAKAQLAQQQARVEQTRREAARLESLLATQSISQREADNASSDQ
                     ALAIAGLAQAEAGVREAQLNLSYTNVTSPLSGIAGRFEFSEGALVNANTSLLTKVSQV
                     NPIWVRFSLSDSELAQLGGHLSPANVKAVTLLTSDGKEYASKGTLNFAASAIEPTLGT
                     QQLRATFENANKQLLPGQFVRVRVTTGYQEGVFVVPQTAVMTNDKGKFVYVAEKDKEN
                     KTVAAIRLIQAGGWQGTHWVVLSGLKEGEQVIIDNLLKVQPGAEIQPNIVGAAPESSP
                     ASQNQPADKG"
     gene            1449..2069
                     /locus_tag="PKL42_10110"
     CDS             1449..2069
                     /locus_tag="PKL42_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNU67468.1"
                     /translation="MVRKSKEDAELTRKRIITAAREVFLSRGVSRTTLEHIATQANVT
                     RGAIYWHFKNKTEIFQAIREQVLLPLIDRMDDSLTLEGNDDPLTQIEKSLCQTIDDLN
                     DNIEMRETYEIIMIKCEYVEDFSEVLQQILENCSSILEKLVLGYQRAQAQNLLACTLS
                     PLALAQDTHLFFSGLLHMWVKDADGSRFRNQAKSLIESHINLRRKH"
BASE COUNT          619 a          546 c          466 g          658 t
ORIGIN      
        1 atgtctttta tcctttatct gctggctgat tttggcttgc aggacttgat tcaggtgcag
       61 cccctactat atttggctga atttctgcgc caggttgcac tttcagcaaa ttatcaataa
      121 tcacttgctc gccttctttt aaaccgctta acaccaccca atgggtgcct tgccatccgc
      181 ctgcttgaat taaacgaatt gccgcaacgg ttttattctc tttatctttt tctgcaacgt
      241 aaacaaattt acctttgtca ttggtcatca cggctgtttg cggcaccaca aacacacctt
      301 cttggtagcc tgtcgtcacg cgtacacgca caaattgacc aggcaacaat tgtttattgg
      361 cattttcaaa ggtagcacgt aattgttgtg tgcctaacgt tggttcaatc gcgctcgcag
      421 caaaattgag cgttcccttg gaagcatatt cttttccatc cgaagtcagt aacgtcactg
      481 ctttcacatt cgcgggcgac aagtggccac ctaattgtgc taactcgcta tcagacaaac
      541 taaaccgcac ccagatgggg ttcacctgac taactttagt gagcaagctg gtattggcgt
      601 tcaccaatgc gccttcagaa aactcaaaac gtccggcaat gccagaaagt ggcgaggtaa
      661 cgttggtgta cgataagtta agttgtgctt cacgcacgcc tgcctcagct tgcgccagcc
      721 cggcaatggc aagtgcttgg tcagaactgg cattatccgc ttcacgttga ctaatagact
      781 gcgttgccaa taagctttct aaacgcgcgg cttcacgtcg ggtttgctca acgcgtgcct
      841 gctgttgcgc cagctgtgct ttggcgttgg ccaaggctat ttgaaacggc accgggtcga
      901 ttaaaaacat caactgccct gcttttacag gtgcgccttc ttcaaacatt ttttttaaca
      961 aaataccgcc cacacgcggg cgaatttcca cctcttttgc gccttcggtc tgggcgacgg
     1021 cttcagcact aatcggcacg cttactgctt ctagcttcaa cacactaaca ggcattggcg
     1081 gcatattgcc acttgcttgc tctgcgggtt ctttttttgt gcaaccaatc accaccatgc
     1141 ctgcaatcac gaccaacata atggtgcgaa aatacctaga gattgataag tgttgtgttg
     1201 ataatttcgc gaacatccta aactccaagc attgagtggt gatgaaaatt ataagcagat
     1261 acattttata gaaaaccaat ttttataaaa accaacgcat acttgcttac tagattgaat
     1321 gaaggtatat tatatacaaa caagaatgta tgtatatatg ttttcaacat tttatttttt
     1381 catatttaac tgaatctatt ttccactgaa aattcaacca aaagtgggta gagcgagcgc
     1441 tattttatat ggtaagaaaa tctaaagaag atgcagaact cacacgaaag cgcatcatta
     1501 cggctgcgcg cgaggtattt ttatcgcgtg gtgtcagccg caccacgctc gagcatatcg
     1561 caacacaggc caatgtaacg cgcggtgcaa tttattggca ttttaaaaat aaaactgaaa
     1621 tttttcaggc catccgcgag caagtgctat tgccgctcat tgatcgcatg gatgacagcc
     1681 tgacacttga aggcaatgat gacccgctta cgcaaattga aaaaagcctc tgtcaaacaa
     1741 tcgatgattt aaatgacaat attgaaatgc gtgagaccta tgaaattatc atgattaagt
     1801 gtgaatatgt ggaagacttt tctgaagtgc tgcaacaaat tttagaaaac tgttccagca
     1861 ttttagaaaa attggtgcta ggttaccagc gcgcgcaagc acaaaatctt ttggcatgca
     1921 cactctcccc gcttgcgctg gcgcaagaca cgcatttatt ctttagcggc ttgctccaca
     1981 tgtgggtgaa agatgcggac ggcagtcgct ttcgcaatca ggcaaaatca cttattgaat
     2041 cgcatataaa tttacgcaga aaacactaaa ataaatcgct attttggcag gttttacaac
     2101 acaacccttt tgacctgccc tgcgagcctt tatttataag gctcacagag cattaaattt
     2161 ctctcgggcg tttttaatca attttaagct agtttcttgg tggtccgcca tgtaagtcat
     2221 gaccatccgc cgtgtagatt tccacttcca caaaatcgcc cggcgttaaa ccttccgcat
     2281 cttctaaat
//
LOCUS       DAOQSI010000222         2224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_60625_length_2224_cov_4.543107, whole genome shotgun sequence.
ACCESSION   DAOQSI010000222 DAOQSI010000000
VERSION     DAOQSI010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60625_length_2224_cov_4.543107"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(238..1767)
                     /locus_tag="PKL42_10115"
     CDS             complement(238..1767)
                     /locus_tag="PKL42_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNU67469.1"
                     /translation="MLSFEKYSPQEIKLNLLSGLTVALAMVPEAIAFALIAHVSPLTG
                     LYAAVLVSLITATFGGRPGMISGAAGALAVVMVALVVQHGVEYLFATVVLMGVLQILF
                     AVAKLGKFIRMVPHPVMLGFVNGLAIVIFLAQFGHFKTVAEDGTTAWMSGLPLYTMLG
                     LIALTMAVIYLLPKLTKAIPSTLAGIMVVSLGVTFLNIDTKTVGDLGSIAGGLPNFHI
                     PQVPFNLETFKIIFPYALILAAVGLIETLLTLNLIDDMTDTRGKPNRESMAQGAANVV
                     TGLFGGMGGCAMIGQSMINVNNGALKRLSGIATAVFLLSFVLFASHWIEMIPLAALIG
                     VMFVVAEKTFEWGSFRLYGKVPKPDLLVIILVAGITVIADLAIAVIIGVIFSALVFAW
                     EHAKQISVTTRTNEQGWKVYELKGTLFFASISAFQNLFSPANDPQEVAIDFKNAKVAD
                     HSALAAIDSLAERYQTAGKKLHLKHLSQDCLELLDKAKGMIEVNVLEDPKYHIADNKL
                     G"
     gene            complement(2142..2213)
                     /gene="pqqA"
                     /locus_tag="PKL42_10120"
     CDS             complement(2142..2213)
                     /gene="pqqA"
                     /locus_tag="PKL42_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HNU67470.1"
                     /translation="MWTTPAATEMRFGFEVTMYVCNK"
BASE COUNT          651 a          509 c          450 g          614 t
ORIGIN      
        1 cctggggtcg agggttcaag tccctctttc accaccaata aagacgcccc tttcgtgaga
       61 ttggggcgtt ttcatttggg tcacgtttag ctcaaccttc aacaaaattt aaagcacttt
      121 tttaaacaaa ttttcgtctt ggatattaga catgcataaa aattgatagg tatatgagca
      181 agttattttt cgcaatacca accaagatga gacttcagct ctcatcatca gcatgattta
      241 acctagtttg ttgtcagcaa tatgatattt tgggtcttcc agcacgttca cttctatcat
      301 ccctttggct ttatctaata gctctaaaca gtcttggctc aggtgtttga ggtgcagttt
      361 tttgcccgct gtttgatagc gttctgctaa tgaatcgatg gcagcaaggg ctgagtggtc
      421 agcaactttg gcatttttga aatcaatcgc cacttcttgt gggtcgtttg cgggtgaaaa
      481 cagattctgg aatgctgaaa tggaggcaaa gaatagcgtg cctttcaatt cgtacacttt
      541 ccagccttgt tcatttgtgc gtgttgttac gctgatttgt ttggcatgtt cccacgcaaa
      601 cactagggca gaaaaaataa ccccgataat gaccgcaatt gccaaatctg caatcaccgt
      661 gatgccggca accaggatta tgactaataa atctggcttg ggtactttgc catacaatct
      721 aaaactaccc cattcaaacg ttttttcagc gacgacaaac atcacgccta tcagtgctgc
      781 cagtggaatc atctcaatcc agtgtgaagc aaacagaaca aatgaaagca gaaacacagc
      841 ggtggcaatg cctgacaatc tttttagcgc gccattgttg acgttaatca tactttggcc
      901 aatcatggcg catccaccca tgccgccgaa taagcctgtt accacgtttg cagcgccttg
      961 tgccattgat tcgcggttag gcttgcctct tgtgtcagtc atgtcgtcaa tcaggttaag
     1021 tgtgagtaaa gtttcaatca agccaaccgc agcaagtatt aatgcatacg ggaaaattat
     1081 tttgaaagtt tcaaggttga atggcacttg cggaatatga aaattaggta agccacctgc
     1141 aatggaacct aaatcaccga cggttttagt atcaatattc aaaaaagtaa cgccaagtga
     1201 aaccaccatg atgccggcta aggttgaagg tatggctttg gtcaatttag gtagcaaata
     1261 gatcaccgcc atggtcaagg cgattagccc taacattgta taaagtggca agccgctcat
     1321 ccacgctgtg gttccatctt cagccacggt tttaaagtgc ccaaactgcg ccaaaaatat
     1381 cacaattgcc aaaccattca caaagcccaa catcacagga tgcggcacca tacgaataaa
     1441 tttacctaac ttggcaacgg caaacagtat ttgcaacacg cccattagca ccaccgtagc
     1501 aaataaatac tcaacaccat gctgcaccac caatgccacc atgacaacag ccagcgcacc
     1561 cgctgcgcct gaaatcatgc ctgggcgacc accaaacgtg gcggtgatca atgataccaa
     1621 taccgctgca tataaccctg taagtggcga cacatgcgca atcaaggcaa atgcaatcgc
     1681 ctctggcacc attgccagcg caaccgttaa gccacttaat aaattgagtt taatttcttg
     1741 tggactgtat ttttcaaaac ttaaaataga caacttacgc tccgatgaca aaattttaca
     1801 gcgttatcga cgcacgtcca taatgtccaa taaccgagat aattagtttc tgaataaatt
     1861 aaaatgtgaa ctgcccacac aataacgaat gcaggtctta agaagtgacg gggcgtaact
     1921 cagaacatta cattgaagtt cagttaatat ctttggtttt tttaagggcg tgattatctc
     1981 acgattaggc tatttaacag cgccatccaa gttaaatttc aatctttgtt ggcgcaacga
     2041 atgtgcgtct tgatgagtta aaagtgattg tacttacgca aaagtggcaa caaaaaagcc
     2101 cagtgattgc gcactgggct ttaaagtttg tttggggctt attatttatt gcaaacgtac
     2161 attgttactt caaagccaaa acgcatttct gttgctgctg gtgttgtcca cataatcatt
     2221 ctcc
//
LOCUS       DAOQSI010000223         2148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_64054_length_2148_cov_4.805542, whole genome shotgun sequence.
ACCESSION   DAOQSI010000223 DAOQSI010000000
VERSION     DAOQSI010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64054_length_2148_cov_4.805542"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PKL42_10125"
     CDS             complement(<1..207)
                     /locus_tag="PKL42_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67471.1"
                     /translation="MNAIISPQQTRMVLFDLDGTLVDTAHDLGYALNLQRARHGLADL
                     PHEVIRPYASHGSVGLLSIGFNLTP"
     gene            complement(204..917)
                     /gene="ubiG"
                     /locus_tag="PKL42_10130"
     CDS             complement(204..917)
                     /gene="ubiG"
                     /locus_tag="PKL42_10130"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HNU67472.1"
                     /translation="MNAETLNNADDLELQKFGDLAHKWWDINSEFKPLHEINPLRLNW
                     IDGLAPLKGKRVLDVGCGGGILSESMYFKGATVTGIDLGEKALKVAKLHQLESGAKVD
                     YQYIAVEQLASLQPASFDVVTCMEMLEHVPDPASIVAACAALVKPNGTVFFSTINRNA
                     KSYLYAVIGAEYVLNMLPKGTHDYAKFIKPSELARMIRTSGLDVQATAGMTYNPITKI
                     YALSDEVSVNYMVQAVKNA"
     gene            complement(914..>2148)
                     /locus_tag="PKL42_10135"
     CDS             complement(914..>2148)
                     /locus_tag="PKL42_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HNU67473.1"
                     /translation="QAGKITEICATEFARTKYVSNEVVQLNHQILIPGLINLHTHAAM
                     SLMRGLADDLPLMPWLQQHIWPAEQKLVSPSFVRDGTLLACAEMLSGGVTCFNDMYFF
                     PESAAAAVNQAGMRANLGLVVIEFPSAYANDADDYLQKGFAAHDGWRNNPFITSSIAP
                     HAPYTVSNKTFESVVTYAEQLGLSVHTHLHETLDEIAQSVANFGVRPMQRLADLGLLG
                     PNLIATHCVHLQKHEMDLLAEHGCYVAHCPTSNLKLASGIAPIAQLLAHGVTVGLGTD
                     GAASNNRLDIFAEMRLSALLAKGSTGDAAVLPAHDVLAMATLHAAQALGLDEKIGSIE
                     VGKYADLTAVNLSDFNVNPCYDPISHLIYVCGREHVTHTWVAGELRYSLGAFANIEPN
                     ELKEIMSTWQPKLRQHKR"
BASE COUNT          586 a          547 c          459 g          556 t
ORIGIN      
        1 cggggttaaa ttaaagccaa tggataacaa accgactgaa ccatgtgaag cataaggacg
       61 aatcacctca tgcggtaaat ccgccaaacc atgacgtgcg cgctgcaaat tcagggcgta
      121 acctaaatcg tgtgcggtat ctaccaaagt gccgtctaaa tcaaacagca ccatgcgcgt
      181 ttgttgcggt gaaattatcg cattcatgcg tttttcaccg cttgcaccat gtaattcaca
      241 ctgacctcat cagatagcgc gtaaattttt gtgattgggt tgtaagtcat gccagcagtt
      301 gcctgaacat caagcccact ggtgcgtatc atgcgcgcta actctgaggg cttaataaat
      361 tttgcataat catgtgtgcc ttttggcagc atattcaata catattcggc accaatcaca
      421 gcataaaggt atgattttgc gttacggtta atggttgaaa aaaacactgt tccattcggt
      481 tttaccagcg ccgcacaggc tgcaacgatg ctggcagggt caggcacatg ttccagcatt
      541 tccatacaag tgactacatc aaagcttgca ggttgaaggc tggctaattg ttcaacggca
      601 atatattgat aatcaacttt tgcaccgctt tctagctggt gtaattttgc caccttaagc
      661 gccttttcgc ccaagtcaat tccggtcacc gttgcgccct taaagtacat cgattcagat
      721 aaaatgccgc cgccgcagcc aacatcgagt acacgcttac ctttaagcgg cgctaaacca
      781 tcaatccagt ttagccgcaa cgggttgatt tcgtgcaacg gcttgaattc actgttaata
      841 tcccaccact tatgggctaa atcgccaaac ttctgaagtt caaggtcatc tgcgttattt
      901 aaagtttctg cattcatcgt ttatgctgcc ttaattttgg ttgccacgta ctcatgattt
      961 cttttaattc attcggttca atattggcaa aggcgcctaa actatagcgc aactcgcccg
     1021 ccacccacgt atgcgtcaca tgttcgcgcc cacaaacgta aattaaatgc gaaatggggt
     1081 cataacatgg gtttacatta aaatcactta aattcactgc ggttaagtct gcatacttgc
     1141 caacttcaat ggacccaatt ttttcgtcaa ggcccagcgc ctgcgcagcg tggagggtgg
     1201 ccatggccaa cacatcatgc gcaggcaata ctgccgcatc gcccgtgctg ccttttgcta
     1261 acaatgcact taaacgcatt tcggcaaaaa tatctaatcg attattgctt gcggcgccgt
     1321 cagtgcccaa accaacggta acaccatgtg ccaataattg tgctataggc gcaataccgc
     1381 tcgctaactt taagtttgaa gtggggcaat gtgccacgta acaaccatgt tcagccagca
     1441 aatccatttc atgcttctgc aaatgcacac aatgggttgc aatcaaattt gggcctaaca
     1501 agcctaaatc agccaaacgt tgcattggcc gcacaccaaa attggccaca gactgcgcaa
     1561 tttcatcgag cgtttcgtgc aaatgcgtgt gaacgctcag ccccaactgc tcagcataag
     1621 tcaccacact ttcaaatgtt ttattcgaaa ccgtataggg tgcgtgcggt gcgatagagg
     1681 acgtaatgaa tgggttattg cgccagccat catgcgcggc aaaacctttt tgcagataat
     1741 catcggcatc attggcatac gctgatggaa actcaatcac cactaaacca aggttggcgc
     1801 gcattcccgc ttggttcaca gcggcggcgg cagactcagg gaaaaaatac atgtcgttaa
     1861 agcaagtgac gccaccactc aacatttctg cgcaagccaa caaagtgcca tcacgcacaa
     1921 aactaggtga caccagtttt tgttccgcag gccaaatatg ctgttgcaac caaggcatga
     1981 gcggtaaatc atctgccaag ccacgcatta agctcatggc cgcatgcgta tgtaaattaa
     2041 ttaaacccgg tatcagtatt tggtggttga gttgcaccac ttcgttcgaa acatacttgg
     2101 tgcgcgcaaa ctcggtcgcg caaatctctg tgatttttcc agcctgaa
//
LOCUS       DAOQSI010000224         2135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_64707_length_2135_cov_5.442788, whole genome shotgun sequence.
ACCESSION   DAOQSI010000224 DAOQSI010000000
VERSION     DAOQSI010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64707_length_2135_cov_5.442788"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PKL42_10140"
     CDS             <1..372
                     /locus_tag="PKL42_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau C-terminal
                     domain-containing protein"
                     /protein_id="HNU67474.1"
                     /translation="GNWRGLIDKLKLGLARNLALNSALLSYDEHTLRFAIQDKDKALL
                     SPMYKDKLSLALEQYFGRKIRLEFSVENHVHTPAKQVAEEKAISQSNAESAIHEDDFV
                     KALLNEFGAEIIPNSIKPMTQ"
     gene            401..709
                     /locus_tag="PKL42_10145"
     CDS             401..709
                     /locus_tag="PKL42_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNU67475.1"
                     /translation="MMKGGMGNMLKQAQMMQANMQKVQDQLAATEVEGIASNGMVKVT
                     MTCNNTVKRVVIADDAMDDKETLEDLILIALTDAKAKAEATSQQHMAGVVPAGFKMPF
                     "
     gene            748..1359
                     /gene="recR"
                     /locus_tag="PKL42_10150"
     CDS             748..1359
                     /gene="recR"
                     /locus_tag="PKL42_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNU67476.1"
                     /translation="MRPTDHQNPQSLDQLVEALRCLPGVGPKSALRMAYHLLQRDRTG
                     AQGLANALNGALQRVGHCQLCNTFSESTICPLCASNTRDKNLLCVVEMPTDLMMLELT
                     HSYNGMYFVLMGRLSPLDGIGPKEINLDKLIKRAQDGVVNEVILATNYTNEGEATAHY
                     ISELLKSRGVAVSRIARGLPMGGEIEYVDSGTLSMALLGRKSC"
     gene            complement(1586..>2135)
                     /locus_tag="PKL42_10155"
     CDS             complement(1586..>2135)
                     /locus_tag="PKL42_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNU67477.1"
                     /translation="ADLPHEVIRPYASHGSVGLLSIGFNLTSEDAAFSSMREEYLALY
                     DQVLARAPVLFDGIEALLKQLEAQSLYWGVVTNKPRRFTAPLLQSIGLMSRAACVVCG
                     DDAVEPKPHPATLLLACAQTNIKPQDCWYVGDAERDIQAGRAAKMKTAVAMYGYLDAA
                     DRPAEWGADAFVSAPEEVLKLL"
BASE COUNT          618 a          530 c          488 g          499 t
ORIGIN      
        1 ggcaactggc gtggtctgat tgataagctc aaattaggct tagcacgcaa tctagcgctc
       61 aacagcgcgt tattgagtta tgacgaacac acgttgcggt ttgccattca agacaaagat
      121 aaagcgctac tcagcccaat gtataaagat aagttgagtt tagcgctgga acaatatttt
      181 ggcagaaaaa tccgcttgga attcagtgtc gaaaatcacg tccacacgcc agccaaacaa
      241 gtggctgaag aaaaagccat ttcgcaaagc aacgcagaat cagccattca tgaagatgat
      301 tttgtaaaag cgctactaaa tgaatttggc gctgaaatta tccccaatag tattaaaccg
      361 atgacacaat aacccttcag aaaactacag gagaagcaac atgatgaaag gtggcatggg
      421 caatatgtta aaacaagccc aaatgatgca ggcgaatatg caaaaagtgc aagaccaact
      481 tgccgcaacc gaagtagaag gcattgccag caatggcatg gtgaaggtga ccatgacctg
      541 taacaatacc gtcaaacgtg tcgtcattgc agatgacgcg atggatgaca aggaaaccct
      601 cgaggatttg atactcattg ccttaaccga cgctaaagca aaagcggaag ctacatcgca
      661 gcaacatatg gcaggcgtgg tgccggctgg ctttaaaatg ccgttttaac gtcatttaac
      721 gcataagact acacatctca cctcattttg cgcccaactg accatcaaaa tccgcaatcg
      781 ctcgaccaac tggttgaagc gctacgctgc ctacccggtg ttgggccaaa gtcggcactt
      841 cgtatggcgt atcatctgct gcagcgcgac cgcaccggtg cacaaggctt agcaaatgcg
      901 ctaaatggcg ctttgcagcg cgttgggcat tgccaactgt gcaatacttt tagtgaaagc
      961 acgatttgcc cgctgtgcgc ttcaaacaca cgtgataaaa atttattgtg cgtggttgaa
     1021 atgccaactg atttgatgat gctagaactt acgcatagtt acaacggcat gtactttgta
     1081 ctgatgggtc ggctttcacc gttagatggc atcggcccga aagaaattaa cctcgataaa
     1141 ctcatcaaac gcgcgcagga cggcgttgtc aatgaagtga ttttagccac caattacacc
     1201 aacgaaggtg aggcgaccgc acattacatc agcgaactgc ttaaatcgcg tggcgttgca
     1261 gtgagccgca tcgcacgcgg cctgccgatg ggcggcgaaa ttgaatatgt tgatagcggc
     1321 actttatcca tggcgctgct tgggcgaaaa tcctgttaat acccgcgtcc gcatttctgc
     1381 aattaacgta aaattgactt catcatctac gggtaaaatt cacctatttt tacgccaatt
     1441 tagccacaac cttttttgat gctctgcaag ccttatcaat aaaggcttcc agagcattta
     1501 ttttttctcg tggaatatca tgggcattaa aggtgattat ttcgatgtca aaaaccggcg
     1561 agttgctcac catttgtata acgcactaaa gtaatttgag cacttcttcc ggtgctgata
     1621 caaacgcatc tgcgccccac tctgctggac gatcagccgc atctaggtaa ccatacatcg
     1681 ctaccgcagt tttcatcttt gccgcgcggc ccgcctgaat atcacgttcg gcatcaccaa
     1741 cataccagca atcctgcggc ttaatgttgg tttgtgcgca cgccaataac aaggtggccg
     1801 ggtgaggttt aggctcaacg gcatcatcac cacaaacaac gcatgcggcg cgcgacatta
     1861 aaccgattga ttgcaataag ggtgcagtaa aacggcgcgg cttattggtg acaacgcccc
     1921 aataaagcga ttgtgcttct agctgcttca atagcgcttc aatcccatca aacaacaccg
     1981 gcgcgcgcgc cagcacttgg tcataaagcg ctaggtattc ttcacgcatg ctactgaacg
     2041 cggcatcctc cgaggttaaa ttaaagccaa tggataacaa accgactgaa ccatgcgaag
     2101 cataaggacg aatcacctca tgcggcaaat ccgcc
//
LOCUS       DAOQSI010000225         2134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_64803_length_2134_cov_2.610871, whole genome shotgun sequence.
ACCESSION   DAOQSI010000225 DAOQSI010000000
VERSION     DAOQSI010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64803_length_2134_cov_2.610871"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..815
                     /locus_tag="PKL42_10160"
     CDS             9..815
                     /locus_tag="PKL42_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNU67478.1"
                     /translation="MISNNRLPIINTSGHTSKLTRYYRIGRVTAHTFVAVLAVSTIFP
                     VASKRVKSTLTKWWCRRLLTTLNMRITTHGHSPLPNSNMRSTMVVANHISWTDIHALN
                     SIEPLRFIAKSEISHWPVFGYLARKANVLFIDRNKRHDAGRIVHKTAKSLEEGDNLCL
                     FPEGTTTDGTKLVPFKSSIIQAAIHAKATIQPVAIRYPTADGTINTHIAYAGDTTMKE
                     SMQQLLMQKNPTVELHFLAPIDLANIDETFLDRRKLTQHIEQLIKEKLDF"
     gene            complement(950..1792)
                     /locus_tag="PKL42_10165"
     CDS             complement(950..1792)
                     /locus_tag="PKL42_10165"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HNU67479.1"
                     /translation="MTTYAIGDIQGCYHAFQALLARIAFNPKTDQLWLVGDLVNRGSG
                     SLEVLRWCVAHQHSLKVVLGNHDLHTLVVAAGFVKAKRGDTLEALLQAEDAPVLLDWL
                     RHQHLVYQQDNFLMVHAGLLPQWSAQQAISYAAEVEAALQGADYLRFLENMYGNQPDH
                     WHDDLIGLDRLRVITNAMTRLRVCTKKGEMEFNFKGELPDIPQGYLPWFDVPKRASQA
                     VNVIFGHWSALGLQQRNNVYALDTGCLWGGQLTAMNLETKAITQVESHPLDKSMKLKK
                     HESD"
     gene            complement(1886..>2134)
                     /locus_tag="PKL42_10170"
     CDS             complement(1886..>2134)
                     /locus_tag="PKL42_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNU67480.1"
                     /translation="KSGEEVYKAVCAMCHGTGLMNAPKFGDKGQWEPRIALGYDTLVK
                     NAVNGIRTMPAKGGNASLSDDEVAGAVKYMANEAGAGF"
BASE COUNT          603 a          547 c          427 g          557 t
ORIGIN      
        1 tggcaactgt gatttcaaac aacagattgc caatcatcaa cacaagcggc cacaccagca
       61 agctcacgcg ctactataga ataggccgcg taaccgcaca cacttttgtt gcggtgctgg
      121 ccgtaagcac tatttttccc gttgcgagta aacgagtaaa atcaaccctg accaaatggt
      181 ggtgcaggcg cttgcttacc actttaaaca tgcgcatcac cacgcatggc cattcacctt
      241 tgccaaatag caacatgcgt agcaccatgg tggtcgccaa tcatatttca tggacagata
      301 ttcacgcact caacagcatt gaaccgctgc gatttattgc aaaatcagaa atcagccact
      361 ggccagtgtt tggctatttg gccagaaaag ccaatgtgct gtttattgac cgcaataaac
      421 gccacgatgc tggtcgcatt gtgcataaaa cggctaaaag cctagaagaa ggagacaatt
      481 tatgcctgtt tcctgaaggc accaccaccg atggcacaaa gctagtgcct tttaaaagca
      541 gcatcattca ggccgctatt catgcaaaag ccaccattca gcctgtggcg attcgctacc
      601 caacggccga tggaaccatt aacacccaca ttgcctatgc gggcgacaca acgatgaagg
      661 aatctatgca gcaattattg atgcagaaaa acccaaccgt tgaattgcac tttttagcgc
      721 cgattgattt ggcaaatata gatgaaacct tcttagaccg ccgaaaactc acccaacaca
      781 tcgagcagtt gattaaagaa aaattagatt tttagtgcgc ttaaacgctt gcatttgctc
      841 actaaaatat catcaattca agcttaaaat ttcccgagaa aaaaataatg ctctggaagc
      901 cttataaaaa aaggctcgca gggcatcgaa aaaggttgca ggttaatttt caatctgatt
      961 catgcttttt aagcttcatc gatttgtcga gcggatgcga ctcaacttgt gtaattgctt
     1021 tggtttctaa gttcattgcc gtcagttgac cgccccacaa gcagcccgta tcgagcgcgt
     1081 aaacattgtt tctttgttgt aaaccgagcg ctgaccaatg gccaaaaatc acatttaccg
     1141 cttgcgatgc acgctttggc acatcaaacc acggcagata cccttgcgga atatctggta
     1201 gttcgccttt aaaattaaac tccatttcgc ctttttttgt acacacgcgc aaacgcgtca
     1261 tcgcgttggt aattacccgc aggcggtcta ggccaattaa gtcatcatgc caatggtctg
     1321 gctgattacc atacatgttt tctaaaaacc ttaaataatc agccccttgc aaggcggctt
     1381 caacttctgc cgcgtaactg atggcttgct gtgcagacca ttgcggcaac aaaccagcgt
     1441 gcaccattaa aaaattatct tgttgataaa ccaagtgctg gtggcgtagc caatcaagca
     1501 gcactggcgc atcttcagct tgcaacagtg cctctaacgt atcgcctcgt ttggctttta
     1561 caaacccggc agccaccact aatgtatgta aatcatggtt gccaagcact acttttaggc
     1621 tatgttgatg tgccacgcac caacgcaaca cttcgagcga accgctgccg cgattgacta
     1681 aatctcccac cagccaaagt tggtcagtct ttgggttaaa tgcaatgcgc gctaacagcg
     1741 cctgaaaagc atgataacag ccttgaatgt cgccaatggc gtaagtggtc atgatttaat
     1801 taccattaaa aaagccactt aattgcttaa gtggcttgtg gattttttca attaccagaa
     1861 aacgtgcagt gaattgaatt ttgttttaaa aacctgcacc agcctcattt gccatgtatt
     1921 ttaccgcacc ggcaacttca tcatcactta aactggcatt gccccctttt gccggcatgg
     1981 tgcgaatacc attgactgca ttcttcacca aagtgtcata ccctagtgca atgcgaggct
     2041 cccattgacc tttgtcgcca aattttggtg cattcatcag ccccgtgcca tgacacatcg
     2101 cacaaacggc tttatacact tcttcgccag attt
//
LOCUS       DAOQSI010000226         2130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_64978_length_2130_cov_6.016386, whole genome shotgun sequence.
ACCESSION   DAOQSI010000226 DAOQSI010000000
VERSION     DAOQSI010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64978_length_2130_cov_6.016386"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1561)
                     /locus_tag="PKL42_10175"
     CDS             complement(<1..1561)
                     /locus_tag="PKL42_10175"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNU67481.1"
                     /translation="MRTSQFFFNTQKEAPSDAELLSHKLMVRAGLIKRLGSGLYTWMP
                     LGLRVLRKVETIVRDEMNKAGALELLMPAVQPKELWEETGRWAVFGPQMLKITDRHER
                     EFCFGPTHEEVITDIARSEIRSYKQLPLNFYQIQTKFRDEIRPRFGVMRAREFMMKDA
                     YSFHTSFESLENTYQTMYQAYSNVFTRLGLKFRAVKADTGAIGGDGSHEFHVLADSGE
                     DGLAYCEQSDYAANVELAEAVATGSRAAAAEALKEVETPKQTACEDVAKLLNISVERT
                     VKAIALIANSQFYLALLRGDHNLNEVKVGKLAGLSDFRFATEEEIRANLNCPPGFIGP
                     VAVGANVTVIADKTVALMHDFVCGANKPKYHLSGVNFGRDLPEPSLVADIRNVVEGDA
                     SPDGKGTLSLCRGIEVGHIFQLRTKYSEAMRATYLDENGQTQVMEMGCYGIGVSRIVG
                     AAIEQGNDERGIIFPSSMAPFNVVICPIGFDKSELVQKAAYKLHDDLQAAGIDVLLDD
                     RGERPGVMFAEA"
     gene            complement(1868..>2130)
                     /locus_tag="PKL42_10180"
     CDS             complement(1868..>2130)
                     /locus_tag="PKL42_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNU67482.1"
                     /translation="QLIGIIILNRGVFTQSGEIVADRGIHHHVGNEGWEHICQAMEAL
                     FRRGEFEAGVLLGIEKIGAILAQHSVLIGVNKNEVSNKPVIL"
BASE COUNT          602 a          541 c          426 g          561 t
ORIGIN      
        1 cagcctcggc aaacatcacg ccggggcgct cgccacggtc gtctaataac acatcaatgc
       61 ctgctgcctg caaatcatca tgcaatttgt atgccgcctt ttgtacgagt tctgatttat
      121 caaaaccgat tgggcaaatc acaacattaa aaggcgccat gctgcttgga aaaataatgc
      181 cgcgctcatc gttgccctgc tcgatggccg caccgacaat gcgcgacaca ccgatgccat
      241 agcaacccat ttccatcacc tgcgtctgac cattttcatc cagataagtg gcgcgcatcg
      301 cttcagaata cttggtgcgc aattggaaaa tatggccaac ttctatgcca cggcaaagcg
      361 acaaggtgcc tttgccatct gggctggcat cgccttccac cacatttcgt atatcagcca
      421 ccaaacttgg ttcaggtaaa tcacggccaa aattcacacc gcttaaatga tatttcggtt
      481 tattggcacc acaaacaaaa tcatgcatca gtgctacggt tttatctgca attaccgtta
      541 catttgcacc taccgccaca gggccaataa agcctggcgg gcaattcaaa ttggcgcgaa
      601 tttcttcctc tgtggcaaaa cgaaagtctg acagacctgc caatttacct actttaactt
      661 cattcagatt gtggtcgcca cgcaagagtg ccaaataaaa ttggctattg gcgattaaag
      721 caatcgcttt cacagtgcgc tcaacactga tgttaagcag cttagccaca tcttcacaag
      781 cagtttgttt gggagtttca acttctttta atgcttctgc tgccgctgcg cgactgcccg
      841 ttgccaccgc ctctgccagt tccacgttgg ccgcataatc tgactgctca cagtaggcca
      901 aaccatcctc gccagaatct gccaacacat gaaactcatg cgaaccatca ccgccaatgg
      961 cgcccgtatc ggccttcacc gcgcggaact tcaagcctaa acgcgtaaat acattgctgt
     1021 aggcttggta catggtttga taagtatttt ccagcgattc aaaactggta tgaaaagaat
     1081 aagcatcttt catcataaat tcacgcgcac gcatcacccc aaagcgcggg cgaatttcat
     1141 cacgaaattt cgtttgaatc tgataaaaat tgagcggcaa ctgtttataa cttcgaatct
     1201 cagaccgcgc aatatcagta atcacttctt catgcgtggg gccaaagcaa aattcgcgtt
     1261 catgcctatc cgtaattttt aacatttgcg gtccaaacac tgcccagcgg cccgtttcct
     1321 cccaaagttc tttcggttgc acggcgggca tcaacagttc taatgcgccc gctttattca
     1381 tttcatcgcg cacgatggtt tcaaccttac gcaacacacg caggccaagt ggcatccaag
     1441 tataaagtcc tgagcctaaa cgcttaatca ggccagcccg caccataagt ttatggctga
     1501 gcaactcagc gtctgatgga gcttcttttt gggtgttgaa gaaaaattgt gaggtgcgca
     1561 tgaatattca aatcactatc aattaaaagt gcgaatttta cagagaatta agcacttagt
     1621 cagcaaactt gcttgatgac ctgaatgtgc gtgtgttaat gatcaaaaaa acatggtgcc
     1681 agcgcaatat tcttgacatc aaaaacacca ttaaaaaaac aaaataatgt acgagaaaaa
     1741 aataatgctc tggaagcctt tatttataag gcttccagag catcaaaaaa ggttgcatta
     1801 aaaattgtgc taaaaatgtt attttatgtg aacgttttta agtaaagttc agattgtcat
     1861 taatggctca aagaatgact ggtttattgg acacttcgtt tttattcacg ccaatcagca
     1921 cagaatgttg cgctaaaatg gcgccaattt tttcaatgcc gagcaaaacc ccggcttcaa
     1981 attcgccacg gcgaaatagt gcttccatgg cttggcaaat gtgttcccag ccttcgttac
     2041 ccacatggtg gtgaatgccg cggtcggcaa caatttcccc agattgtgta aaaacgcccc
     2101 gatttagtat aattatacct atcagttgtg
//
LOCUS       DAOQSI010000227         2126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_65205_length_2126_cov_4.083535, whole genome shotgun sequence.
ACCESSION   DAOQSI010000227 DAOQSI010000000
VERSION     DAOQSI010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65205_length_2126_cov_4.083535"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="PKL42_10185"
     CDS             complement(<1..533)
                     /locus_tag="PKL42_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNU67483.1"
                     /translation="MTDQQHAYLVTRLDRLNDIGIALSAERDTKRLLEMILFGAKEIT
                     NADSGTLYILSEDKQFLKFEILSNSTLNVQLGGTTGQEIPLLPIPLYLPDGNPNLTTI
                     AAYATLQSKTVNIVDAYQTEDFDFSGTRKYDQIVGYHSQSFLTVPMKNHEDEIIGVLQ
                     LINALDIHSGEIIPFSEA"
     gene            complement(639..1385)
                     /locus_tag="PKL42_10190"
     CDS             complement(639..1385)
                     /locus_tag="PKL42_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNU67484.1"
                     /translation="MAKLILYLDNAILSEFFLKKELTTIGRRPNNVIHMDNLAVSGEH
                     AVVVKKGNDFYVEDLGSTNGTTVNGQVIKNHLLRHGDVVEIGKYQLKFIDEEVLSHIG
                     TEQAEHAGFEKTVMVHPSALQAFERQAKETANQAFDTTASLEQSHTEVVAPVAAIASP
                     TVGKVQVLNGPSAGRELVLNKALTTLGKVGVQVAVITKRANGYFITHVEGKTYPLVNE
                     VSTGAQAFALNDQDVIEIAGVKMAFYLDAA"
     gene            complement(1400..>2126)
                     /locus_tag="PKL42_10195"
     CDS             complement(1400..>2126)
                     /locus_tag="PKL42_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNU67485.1"
                     /translation="SEIAVLSIAAELTEVMLNQPPLESDQYLQYQDQALNAMQPEAQL
                     LVDAVKNANDAIYYVSQTQSQCAGMGTTLVLGLFINNRLLVGHIGDSRMYRLRGLEFT
                     QLTEDHSILQEQLNAGLITAEQAKYSVNRNLVTRALGVDPEVPLELSEFDVEVGDIYL
                     LCSDGLTDMVDDNQIQLILSMPDIGLDSRAQRLIQLANEHGGKDNISVILVRVNKTFE
                     KKRPKRKNLGLNHHMDNFFGWLK"
BASE COUNT          579 a          496 c          419 g          632 t
ORIGIN      
        1 gcttcagaaa acgggataat ttccccgcta tgaatatcta gcgcattgat gagttgtaac
       61 acgccgataa tttcatcttc atgatttttc atcggcactg tcaaaaatga ctgtgagtga
      121 tagccgacaa tttggtcata tttgcgcgtg ccagaaaaat caaaatcttc agtttgataa
      181 gcatccacaa tattcaccgt tttactttgc agggttgcat aagcagcaat ggtggttaaa
      241 ttcgggttgc catcaggtaa atataacggt atcggcaata aaggaatctc ttggcctgtg
      301 gtaccgccca attgcacatt gagcgttgag ttacttaaaa tttcaaactt taaaaactgc
      361 ttatcttcag acaatatata caacgtgccg ctatcagcgt tggtaatttc ttttgcgcca
      421 aacaaaatca tttctagcaa acgtttagta tcgcgctcgg ctgaaagcgc aatgccaatg
      481 tcgtttaggc gatcaagccg tgtgactaaa taagcgtgct gttgatccgt catatgaggt
      541 ggctaaaaag acagaaatac tggtaaattc atcatattag aaaaataaaa tcaatcgtta
      601 agatagcatg ttgaaaatgt gtttcgtgta aattattgtt aagctgcatc aaggtaaaac
      661 gccatcttca cacccgcaat ttcaatcaca tcttggtcgt tcagggcaaa agcctgcgca
      721 ccggtagaaa cttcattcac taaggggtaa gttttgcctt caacatgggt aataaaataa
      781 ccattcgcac gcttagtaat cacggccacc tgcaccccta ctttgcccag tgttgtgagc
      841 gctttattga gtaccaactc tcggccagcg cttgggccgt taagcacctg tactttgcct
      901 accgttgggc tggcaattgc cgcaactggc gccacgactt cagtatgcga ttgttccaaa
      961 gaggctgtgg tgtcaaacgc ctgattggcc gtttccttcg cttggcgctc aaatgcttgc
     1021 aatgccgacg gatgcaccat caccgttttt tcaaaaccgg cgtgttcggc ttgctcggta
     1081 ccgatgtggc tcaatacttc ttcatcaata aactttaatt ggtatttgcc tatttcaacc
     1141 acatcgccat gacgcaataa atgattcttt atcacttgcc cgttcacggt agtgccattg
     1201 gtactgccta gatcttctac ataaaaatcg ttaccttttt ttacgaccac cgcatgctca
     1261 ccgctcacag cgaggttatc catatgaatc acattgttgg gtctgcggcc aatcgtcgtt
     1321 agctcttttt ttagaaaaaa ttcacttaat atcgcgttat ccaaataaag tattaatttc
     1381 gccatttttt ataatcctgc tattttaacc aaccaaaaaa gttatccatg tggtgattta
     1441 accccaaatt tttgcgcttt ggacgctttt tttcaaaagt tttattaacc cgcactagta
     1501 tgacagaaat attatcttta ccgccatgct cgtttgccag ttgaatcaag cgttgcgccc
     1561 tgctatccaa gcctatatcg ggcatgctta aaatcaattg aatctgattg tcgtccacca
     1621 tatccgttaa gccatcagaa cataacaaat aaatatcacc cacttccaca tcaaactcgc
     1681 ttaattctaa cggcacttca gggtctacgc ctaaagcgcg cgtgaccaaa ttgcgattca
     1741 ctgaatattt tgcctgctct gcagtgatta atccggcgtt gagctgctct tgcaggattg
     1801 agtggtcttc tgtcaattgc gtaaactcaa ggccgcgcaa gcgatacatg cgtgaatcac
     1861 caatatgacc gaccaataat ctgttattga taaacaaccc caagaccaat gttgttccca
     1921 ttccagcaca ttgcgactgt gtctgcgaaa cataataaat ggcatcattg gcatttttta
     1981 ccgcatctac tagcagctgc gcctctggct gcatggcgtt aagcgcttgg tcttgatatt
     2041 gcagatattg atctgactct aaggggggct gattgagcat tacttctgtg agttcagcgg
     2101 caatgctgag cacggcaatt tcacta
//
LOCUS       DAOQSI010000228         2118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_65588_length_2118_cov_5.436743, whole genome shotgun sequence.
ACCESSION   DAOQSI010000228 DAOQSI010000000
VERSION     DAOQSI010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65588_length_2118_cov_5.436743"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..1284
                     /locus_tag="PKL42_10200"
     CDS             181..1284
                     /locus_tag="PKL42_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNU67486.1"
                     /translation="MAAIDISPILKFMLEKNGSDLFFSTGAAIHIKVEGDTLPINAQI
                     MSQGMVKEIAYSIMTPAQISEFENTLECNFAIGQRDLGRFRVNVFKQRGELGMVVRHI
                     KTEIPTIESLGLPPILSKLIMRKRGLILVVGATGSGKTTSLASMIDYRNANATGHIVT
                     VEDPIEFMYSHKKSVVDQREVGIDTLSFENALKNAMRQAPDVILIGEIRDMEGMKNAL
                     AYAETGHLCLATLHANNANQALERIISFFPEQDRSGLLLGLSMNLISIVSQRLISGIQ
                     SKRIPAVEVMINTPYISELIQKQKMSEIKTMMADSNDIGMMTFDQSLFKLFTNGLIDE
                     ENALTNADSRNDLSLKIRFAAEAGRSMPSSFGE"
     gene            complement(1292..>2118)
                     /locus_tag="PKL42_10205"
     CDS             complement(1292..>2118)
                     /locus_tag="PKL42_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNU67487.1"
                     /translation="GVIWYPYRIMAEAGVSGVAASFYTYFLAIILASIFYLRHWRGIF
                     SQPSTIIWLSLAAGWTNLSYVLAVIHGEVMRVMLLFYLSPIWTLLLAHFWLKEKTHFT
                     GYVAIGISLLGAFVMLYNPQLGGLPLPNNNAEWLALASGIGFSLTNVITRKSSHLSLA
                     AKSFSVWVGVMLVAALSMPFIDGKFASPNLFTATNWVVMGVIAILLLAATLFVQYGVT
                     RIAATRASILFLFELVVAAIASYYLANEQMSVNEWIGGGLIVFAAIFAASQHSNTS"
BASE COUNT          687 a          474 c          421 g          536 t
ORIGIN      
        1 gcttttatca ccatgtacaa cgccaagcta aaataatgca tattgtgata atttaagaaa
       61 aataatgaga aacctaatgt aaactaatga taactatagc gaatcgtgtt atttttatgc
      121 taaagttagc cctgtatcat tgatcgttta ttagactcaa gtcgcactta ggaacgcacc
      181 atggcagcaa tcgacattag ccccatctta aaatttatgt tagaaaaaaa tggctctgat
      241 ctgtttttca gcaccggggc agccattcat attaaagttg aaggcgatac gctgcctatc
      301 aatgcacaaa ttatgtcgca gggcatggtt aaagaaatcg cctactcgat tatgacaccc
      361 gcgcaaatta gcgagtttga aaatacgctg gagtgtaact ttgccattgg gcagcgcgat
      421 cttggtcgct ttcgggtgaa tgtctttaaa caacgcggtg aattaggcat ggtagttcgt
      481 catattaaaa ctgagattcc aactattgaa agcttagggc tgccaccaat tttaagtaaa
      541 ctcataatgc gcaaacgtgg cctgattctt gtggttggcg ctacgggctc aggtaaaact
      601 acttcgcttg cttccatgat tgattatcga aatgccaacg cgaccgggca tattgtcacc
      661 gttgaagacc ctattgaatt tatgtattct cataaaaagt cagtggtaga ccagcgcgag
      721 gtaggtattg acacgctttc ttttgaaaac gcgcttaaaa atgccatgcg ccaagcgcca
      781 gatgtaattt taattggtga aattcgcgac atggaaggca tgaaaaacgc cctcgcgtac
      841 gctgaaacag gccatttatg tttagccacc ctacatgcca ataacgccaa tcaggcgctt
      901 gagcgtatta tttcattttt tccagaacaa gaccgaagcg gcttgctact tggtttatcc
      961 atgaatttaa tttcgattgt ttcgcaacgt ttaatttcag gcatacaaag caagcgcata
     1021 cccgccgttg aggtaatgat taacactcca tatatttcag aactcattca gaaacaaaaa
     1081 atgagcgaaa tcaaaaccat gatggccgat agtaacgata ttggcatgat gacatttgac
     1141 cagtcactgt ttaaattatt taccaacggc ctgattgatg aagaaaacgc gctaacaaat
     1201 gcggactcac gcaatgacct ttcgttaaaa atccgctttg cagcagaagc tgggcggagc
     1261 atgccaagtt cttttgggga gtaatccaaa actaagaagt atttgaatgt tgacttgcag
     1321 caaatattgc ggcaaataca atcaaaccgc caccaatcca ttcattgaca ctcatttgct
     1381 cgttggccaa ataatacgat gcaatggcag ccacaacgag ttcaaacaaa aatagtatcg
     1441 aagcccttgt tgcagcaatg cgtgtaacac catactgcac aaaaagcgtt gctgctaaca
     1501 acaatattgc aataactccc atcacaaccc agtttgtagc cgtgaatagg tttggagatg
     1561 caaatttacc atcaataaaa ggcatagata atgccgccac cagcattacc ccaacccaaa
     1621 ctgagaaaga ctttgcggcc aaagacaaat ggctcgattt tcgagtaatg acattggtca
     1681 gcgaaaagcc aataccagaa gccaatgcca gccattctgc attgttattc ggtaatggta
     1741 aaccacctag ctgagggtta tacagcatca caaacgcacc caacaaagag atgccaatcg
     1801 ccacataacc tgtaaaatgc gttttttctt tcagccaaaa atgtgcaagc aataatgtcc
     1861 atataggcga caaataaaat aacagcatca cacgcataac ttcgccatga atgacggcca
     1921 gcacgtagct taggtttgtc cagccagcgg ccaaactcaa ccatataatg gtactcggct
     1981 gactaaaaat acctcgccaa tgtcttaggt aaaatatgct ggctagaata atggctaaaa
     2041 aataggtgta aaaactagcg gcaacccccg aaacaccagc ctcagccatg atgcgataag
     2101 gataccaaat cacgcccc
//
LOCUS       DAOQSI010000229         2078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_67702_length_2078_cov_4.646565, whole genome shotgun sequence.
ACCESSION   DAOQSI010000229 DAOQSI010000000
VERSION     DAOQSI010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67702_length_2078_cov_4.646565"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PKL42_10210"
     CDS             complement(<1..423)
                     /locus_tag="PKL42_10210"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148032.1"
                     /note="FabH; beta-ketoacyl-acyl carrier protein synthase
                     III; catalyzes the condensation of acetyl-CoA with
                     malonyl-ACP to initiate cycles of fatty acid elongation;
                     differs from 3-oxoacyl-(acyl carrier protein) synthase I
                     and II in that it utilizes CoA thioesters as primers
                     rather than acyl-ACPs; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HNU67488.1"
                     /translation="MTNSRNIYSRIAGTGSYLPEKLLTNADLEHMVDTTDEWIFTRTG
                     IRERHLIADDQMTSDLALHAARNAIESANIALTDIDLIIVATTTPDKIFPSTAVILQN
                     KLGISGCPAFDIQAVCSGFVYALATADNFIKSGAAKCAL"
     gene            complement(420..1493)
                     /gene="plsX"
                     /locus_tag="PKL42_10215"
     CDS             complement(420..1493)
                     /gene="plsX"
                     /locus_tag="PKL42_10215"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNU67489.1"
                     /translation="MDITLSVDAMGGDHGPHVTIPAVLKALDEDAQLNVVLVGLSDAI
                     EAELKARKASTGPRLRIHPATEVVTMDEAPQSALKNKKDSSMRVAINLVKSGEANACV
                     SAGNTGALMATARFVLKTLPGIDRPAIAGVFPSQKGKTYILDLGANADSTPEQLLQFA
                     VMGSMLVSSIANKAKPTVGLLNIGTEDIKGNEVVKQAGELLRASHLNFYGNVEGNDIF
                     KGTTDLVVCDGFVGNVTLKASEGVAQMMVRFLSEEFKRNWLTKLMGLIAMPVLKAFKK
                     RLDPRLYNGASFLGLRGIVVKSHGGADIVGFKNAIHIAVEEARSGVLRRITEQLEIEH
                     IQSQTQSNGDTAFGVEANHLENA"
     gene            complement(1693..1872)
                     /gene="rpmF"
                     /locus_tag="PKL42_10220"
     CDS             complement(1693..1872)
                     /gene="rpmF"
                     /locus_tag="PKL42_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNU67490.1"
                     /translation="MAVQQNKKTPSKRGMHRSHDFLVNPALAVEPTTGEAHLRHHISP
                     NGYYRGRKVIATKGE"
BASE COUNT          583 a          504 c          424 g          567 t
ORIGIN      
        1 cagcgcacac ttggccgccc ctgatttaat aaagttatct gcggttgcga gcgcatatac
       61 aaaaccgcta caaaccgcct gaatatcaaa cgctgggcag cctgaaatac ccagtttatt
      121 ttgcaatatt accgcagtgc taggaaaaat tttgtcgggc gtggtcgtcg caacaataat
      181 taaatcgatg tcagtcaatg caatatttgc cgattcaatg gcatttcgcg cagcatgcaa
      241 tgccaaatcg cttgtcattt ggtcatctgc aattaaatgg cgttcacgta tgccggtacg
      301 cgtaaaaatc cactcatctg tggtatcaac catatgctct aaatcggcat tggtgagtaa
      361 tttttcaggt aaatagcttc ccgtacccgc aatacgcgaa taaatgttgc gtgaatttgt
      421 catgcgtttt ccaagtgatt cgcttcaacg ccaaacgctg tatcaccgtt tgactgggtt
      481 tgcgattgta tatgttcaat ttctaactgt tcagtaatgc ggcgtagcac accactacgg
      541 gcttcttcca cagcaatgtg tatggcgttt ttaaagccta caatatcggc accaccatga
      601 ctttttacca caatgccgcg taagcctaaa aagctcgcgc cattatataa acgtgggtct
      661 agccgttttt taaatgcctt caacacaggc atggcaatca gccccatcag tttagtcaac
      721 cagttacgtt tgaactcttc tgacaaaaaa cgcaccatca tctgcgcaac gccctctgaa
      781 gccttcaatg tcacgttacc gacaaaacca tcacaaacga ccaaatcagt ggtgccttta
      841 aaaatatcat tgccctctac gttgccataa aagttcaaat ggcttgcgcg cagcaattcg
      901 ccagcctgct tcaccacttc attaccttta atatcttcag tgccaatatt gagcaagcca
      961 accgttggtt ttgctttatt tgcgatggag gacactaaca tgctacccat caccgcaaat
     1021 tgcaataatt gttcaggcgt actatctgca ttagcaccta aatccagaat ataggtttta
     1081 cccttttggc tggggaaaac gcctgcaatc gctgggcgat caatgcctgg caaggttttg
     1141 agtacaaagc gtgcagtggc cattagcgcg ccggtattgc ccgcacttac acaagcattg
     1201 gcctcgccgc tttttaccaa attaatggcc acgcgcatgg aagaatcttt tttatttttg
     1261 agtgcactct gcggggcttc atccatcgtc acgacttcag tggcaggatg aatgcgtaaa
     1321 cgcggaccag tgctagcctt gcgcgctttg agttcggctt ctatcgcgtc actcaaaccc
     1381 accagcacaa cattgagttg cgcatcttcg tcgagcgcct tcagcactgc gggaatcgtc
     1441 acgtgcgggc cgtggtcacc gcccatagca tcgacagaaa gggtaatatc cattggttta
     1501 atcagcctat tcgaaaatca caccgtaaaa tagaacatag cgaaataaaa catgacaaac
     1561 gctagataca ctaacgccgc gccaaaattg gtgcggcgtt aaaattggtt taagcgcaga
     1621 aagtcgcaat ttgtttattt aatccaatat tagcgtgggc taatattagc attagataca
     1681 aacaaattga aattattcgc ctttggttgc aatcacttta cggccacggt agtaaccgtt
     1741 tgggctgatg tggtgacgca aatgtgcctc gcctgttgtt ggctcaacgg ccaatgccgg
     1801 gttgactaaa aagtcatgtg aacgatgcat accgcgttta gacggtgttt ttttgttttg
     1861 ctgaactgcc ataataaaac tccaataaaa tgattccaag aaacggatta aagccatcat
     1921 cttaattaca aattaactgg tgcttgacta acttttgcac cgcgaattaa agactacgtt
     1981 aaactactag cccggcacta taatataaaa gctgactttt gtcagccaaa atcacacttt
     2041 agccactaaa aaatagatgc gatatcaata tattgcgg
//
LOCUS       DAOQSI010000230         2052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_69104_length_2052_cov_5.525288, whole genome shotgun sequence.
ACCESSION   DAOQSI010000230 DAOQSI010000000
VERSION     DAOQSI010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69104_length_2052_cov_5.525288"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="PKL42_10225"
     CDS             complement(<1..737)
                     /locus_tag="PKL42_10225"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HNU67491.1"
                     /translation="MQTQWRYFLTAVMFFTRVPVHFEQFVASDLNRATRFFPLVGILV
                     GAIAALAFWIFQFVLPSEIAVILSMVTSILLTGAFHEDGLADAIDGLGGGWEREQVLA
                     IMVDSRIGSYGAIGLVMVLLAKFQALIHLDVWLIPIVLVAGHALSRWCAVVVMATQHY
                     VKAEGKSKPLATQLSWLELAIATFFGLVPLLFVGVAGLLALLPVFFVWVWFSLKIKRR
                     IGGYTGDCLGAMQQLTEVTFYIGLLAS"
     gene            complement(746..1783)
                     /gene="cobT"
                     /locus_tag="PKL42_10230"
     CDS             complement(746..1783)
                     /gene="cobT"
                     /locus_tag="PKL42_10230"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HNU67492.1"
                     /translation="MNFIITKPNQALKSALVDKINHKTKPLGALGMIEAVALQIGLMQ
                     QSLEPKLTHPTMLVFAGDHGIVEAGVSPYPQAVTAQMVLNFLQGGAAINVFTTQNNIR
                     LRVVDAGVNHQFADNANLINAKIAPGTRNFLAEAAMTTSQCESAIEKGAKIVDAEIAA
                     GCNVFAFGEMGIGNTSSASCLMSVLCGLPLEDCVGRGTGLDDAGLHHKTNILAQAIAH
                     HALNQDDAEQVLATFGGFEIAMMVGAMLQAAASGCTLLIDGFITTAALLVAAKLQPNI
                     LHYCVFTHCSNEAGHQKMLAFLNVKPLLNIDLRLGEGTGAALAYPLVNASVNFLNQMA
                     SFESAKVSQKT"
     gene            1977..2052
                     /locus_tag="PKL42_10235"
     tRNA            1977..2052
                     /locus_tag="PKL42_10235"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2010..2012,aa:Phe,seq:gaa)
BASE COUNT          598 a          520 c          418 g          516 t
ORIGIN      
        1 aaacttgcaa gcaaaccaat ataaaaggtg acttcggtga gctgttgcat tgcgcctagg
       61 cagtcaccgg tatatccgcc gatacggcgt ttaattttca gactgaacca aacccagaca
      121 aaaaacactg gcaatagcgc taacaaccct gcaacgccta caaataacaa agggaccaat
      181 ccaaaaaaag tagctattgc caactcaagc caagataatt gtgtggcaag tggcttagat
      241 ttcccttcag cctttacata gtgctgcgtt gccattacca cgacagcgca ccatcgactc
      301 aatgcatgac ctgcaactag tacgattgga atcaaccaca catctaaatg aatcagggcc
      361 tgaaatttag ccagcagcac catcaccaag ccgattgcac cataactgcc aatgcgcgaa
      421 tccaccataa tagcgagcac ttgctcacgc tcccatccac cacctaagcc atcaattgca
      481 tctgccaagc catcttcatg aaaagcgccg gtgagtaaaa ttgaagtgac catgctcaaa
      541 attacggcta tttctgaggg caaaacaaac tgaaaaatcc aaaaggctaa tgcggcaatc
      601 gcgccaacta agatgcccac cagtgggaaa aatcgcgtcg cgcgatttaa atcagacgcc
      661 acaaactgct caaagtgcac gggcacgcgt gtaaaaaaca ttaccgctgt taaaaagtaa
      721 cgccattggg tttgcatttg gcgcatcatg ttttttgcga aactttggcg gattcaaacg
      781 aagccatctg atttaaaaaa ttcaccgatg cattcactag cgggtaagct aaggccgcgc
      841 ctgtaccttc gcctaatctt aaatctatat tcagtagtgg cttaacattt aaaaatgcga
      901 gcattttttg atgtccggcc tcatttgagc aatgcgtaaa cacgcaataa tgcaatatgt
      961 tgggctgcaa tttggccgca accagcagcg ctgcggttgt gataaaccca tcaatcaaca
     1021 aagtacaacc actcgccgct gcctgtagca tggcacccac catcatggca atttcaaagc
     1081 caccaaatgt cgccagcact tgctcggcat catcttgatt gagtgcatga tgcgcgatag
     1141 cttgcgccaa aatattagtt ttgtgatgta aacctgcgtc atccagaccc gtgcctcgcc
     1201 ccacgcaatc ctctaaaggc aaaccgcaca gcacgctcat taaacaactt gctgaagaag
     1261 tgttaccaat gcccatttca ccaaacgcaa acacattgca acctgcggct atttcagcat
     1321 caacgatttt tgcacctttt tctatggcac tctcacattg cgaagttgtc attgcagctt
     1381 cagctaaaaa attacgcgta cctggcgcaa ttttggcgtt gattaagttg gcgttatctg
     1441 caaattgatg gtttactcca gcatctacaa cgcgtaaccg aatgttattt tgggtagtaa
     1501 atacgttaat tgccgctccg ccttgcaaaa aattaagaac catctgtgct gtgacggctt
     1561 gaggataggg gctgacacct gcttcaacaa ttccatgatc gccagcaaat accagcattg
     1621 taggatgcgt gagttttggt tcgagcgatt gttgcatgag tccaatttgc aacgcgactg
     1681 cctctatcat gcccaatgcc cctaatggct ttgttttatg gttgattttg tcaacaagcg
     1741 cacttttgag ggcttggtta ggcttagtta tgatgaaatt catagccgaa ttttacatga
     1801 gcaacactac aaattcatta gtgctcatct aaatatgttc accacacgcc caaaaggcac
     1861 tctgcgtaca gatgacaacc gataagactt catataattc agcaattatc tttggaagtt
     1921 ttttggaagt ttgctataat tcgcgccttg caaacattta ccgacgtttg catgatggcc
     1981 gggtagctca gtcggtagag cagcggattg aaaatccgcg tgtcacaggt tcgattcccg
     2041 tcccggccac ca
//
LOCUS       DAOQSI010000231         2043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_69572_length_2043_cov_4.443662, whole genome shotgun sequence.
ACCESSION   DAOQSI010000231 DAOQSI010000000
VERSION     DAOQSI010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69572_length_2043_cov_4.443662"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="PKL42_10240"
     CDS             complement(<1..520)
                     /locus_tag="PKL42_10240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNU67493.1"
                     /translation="MIEFKEISKQWPDGTMALSEVSVSIPKGQFCVILGPSGAGKSTL
                     LRAVNGLMQPTNGRIVIDGTALTPATARYLRQRVAMIHQHFNLTLRMSVAGNVLAGLL
                     PVVSTGRAILGWFTPEHRAKACSLLARVGLTPKHVQRRAGELSGGQQQRVGVARAFML
                     DPEVVLADEPVAS"
     gene            complement(585..1517)
                     /locus_tag="PKL42_10245"
     CDS             complement(585..1517)
                     /locus_tag="PKL42_10245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNU67494.1"
                     /translation="MKLKKLLSAMVVASLVHIATPAFAAQNDGSAEKPLRVVLIPADG
                     GTEEGTKKDFQPIFDAISGHTGLKFDIKVGQSYNAVVEAMCTGAADIAWFGPASYLQA
                     RNRGCAELMALAVKGGESVYYSGIFARKDAKINTIADLKGKRIALGDVNSTSSFNVPV
                     AMMLANGVNPAQDASAINMAGSHANVLKALSEGLVDAGGASFDSYEKAVNQKAIDPSK
                     IKVVVKSDPIPQPPLAMHPELSAELKKKLKFSFSNIHNMPGITKEQIRGYGGGKVDGY
                     TALVSEKQMAETGKMFETVTEQVKTEIMQKAGKR"
     gene            complement(1563..>2043)
                     /locus_tag="PKL42_10250"
     CDS             complement(1563..>2043)
                     /locus_tag="PKL42_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNU67495.1"
                     /translation="LGQNTRFTELLEAEGMTAVTRILRKLQIPAHDGVAKRLNLPENA
                     PVIWLETLRLVDDRPYCVISHFLPLQRFPELLESYTGGSLHDFILSNYQISLQRVESL
                     VTATLPLGDDAGLLHMPQHQAILRVKSVNVDAACPEMPLEYALTRFRADRIQLRIRP"
BASE COUNT          531 a          530 c          425 g          557 t
ORIGIN      
        1 agctcgctac aggttcatcc gccaacacca cttcagggtc tagcataaat gcgcgtgcca
       61 ccccaacgcg ctgctgctgc ccgcctgaca actcacctgc acggcgttgt acatgcttcg
      121 gcgtcaagcc aacacgcgcc aacagtgaac aagcttttgc gcgatgttct ggtgtaaacc
      181 agcccaaaat ggcacgtcct gttgaaacca ctggtaacaa gcctgccagc acattacctg
      241 ctacgctcat gcgcaaggtt agattaaagt gttgatgaat catcgccacg cgttgacgca
      301 aataccgcgc tgtggctggg gttaatgctg taccatcaat gacaatacgg ccattggttg
      361 gctgcattag gccgttgacc gcacgcaata atgtagattt acctgcacca gatggcccca
      421 gaatgacaca gaactgccct ttgggaatac tgacactcac ctcagatagt gccatcgtgc
      481 catctggcca ttgttttgaa atttctttaa attcaatcat gtctttctcc cgctaaaata
      541 gggatgacaa aacatttgcc accccttgaa tcaacaacct gcgactagcg tttacctgct
      601 ttttgcataa tctcggtttt gacttgctca gtcaccgttt caaacatttt tcctgtttca
      661 gccatttgct tttcacttac cagcgcagtg tatccatcta ccttgcctcc accgtagcca
      721 cggatttgct ctttagtaat gccaggcata ttgtgaatat tgctaaatga aaacttgagc
      781 ttctttttta actctgcaga gagttcagga tgcatcgcca atggcggttg tggaatagga
      841 tcacttttca cgaccacttt aatcttgctg gggtctatcg ctttttgatt aacagccttt
      901 tcgtatgaat caaaagaggc accgcctgca tccaccaaac cttctgaaag tgctttcaag
      961 acgttggcat gactgcccgc catattgatt gccgaggcat cttgtgctgg gttcacacca
     1021 ttcgccaaca tcatggcaac tggcacatta aagcttgagg ttgagttcac atcacctagc
     1081 gcgattcttt tgccttttag gtcagcaatc gtattgatct tggcatcttt acgcgcaaaa
     1141 atacctgagt aatacacaga ttcgccgcct tttactgcca gtgccatcag ctcagcacag
     1201 ccgcgattac gtgcttgcaa ataagaagct gggccaaacc atgcaatatc tgccgcgcca
     1261 gtacacatcg cctcaaccac tgcgttataa ctttgcccta ctttaatatc aaacttaagg
     1321 ccagtatgcc cagaaattgc atcaaaaata ggttgaaaat ccttttttgt accttcttca
     1381 gtaccaccat cagcaggtat cagcacaaca cgcaatggtt tttctgcact gccgtcattt
     1441 tgtgcagcaa atgctggtgt tgcgatatgc actaatgagg ctacaaccat tgcagacaat
     1501 aattttttta atttcatttg aaacactcct atttgatatt gatatggata agaaaaacta
     1561 acttaaggac gaatacgtaa ctgaatacga tccgcacgaa aacgggttaa ggcatattcc
     1621 agtggcattt ctgggcatgc cgcatcgaca ttcacgcttt tcacacgcaa tatcgcctga
     1681 tgctgcggca tgtgtagcaa accagcgtca tcgcccagtg gcaatgtggc tgtcactaaa
     1741 ctttccaccc tttgtagaga aatttggtag ttgctaagaa taaaatcgtg aagtgatccg
     1801 ccggtataac tttctaacaa ttcaggaaag cgttgtagcg gtagaaagtg agaaattacg
     1861 cagtaaggcc ggtcgtcaac caagcgtaat gtttccagcc aaatcactgg tgcattctct
     1921 ggcagattaa ggcgcttggc cacgccatcg tgtgccggta tttgcaactt ccgcaaaatg
     1981 cgcgtaactg ccgtcatgcc ttctgcttca agcagctcgg taaagcgcgt attctgtccc
     2041 agc
//
LOCUS       DAOQSI010000232         2021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_70754_length_2021_cov_5.626653, whole genome shotgun sequence.
ACCESSION   DAOQSI010000232 DAOQSI010000000
VERSION     DAOQSI010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70754_length_2021_cov_5.626653"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..228)
                     /locus_tag="PKL42_10255"
     tRNA            complement(144..228)
                     /locus_tag="PKL42_10255"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(192..194),aa:Tyr,seq:gta)
     gene            complement(301..376)
                     /locus_tag="PKL42_10260"
     tRNA            complement(301..376)
                     /locus_tag="PKL42_10260"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(341..343),aa:Thr,seq:tgt)
     gene            complement(424..852)
                     /locus_tag="PKL42_10265"
     CDS             complement(424..852)
                     /locus_tag="PKL42_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HNU67496.1"
                     /translation="MTAFTSTKPYMIRAIHEWCVDNGCTPHLLVTVNSRTRVPLAYVK
                     EGEIVLNLNYSATKDLQITNDAITFSARFGGISQNLFVPINAVRGIFARENGQGMFFE
                     IEPDLAPISDDSAQQKPDVEPALNAEKPPSTKKPTLTIVK"
     gene            complement(1033..1632)
                     /locus_tag="PKL42_10270"
     CDS             complement(1033..1632)
                     /locus_tag="PKL42_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HNU67497.1"
                     /translation="MMTLYSGTTDPYSHRCRIVLYEKGMDFQVIDVDLANKPEDLAVI
                     NPYNQVPVLVERDLVLSEANIINEYIDERFPHPQLMPPDPVMRARARLFLYNFEKELF
                     DHIKDIESDNEELADKARKTIADNLTQLVPIFSKQNYLLGDDYSMLDVAITPLLWRLT
                     HYGIELQNQAAPILKYAERLFSRPLYADAMTPSEKVMRK"
     gene            complement(1636..>2021)
                     /locus_tag="PKL42_10275"
     CDS             complement(1636..>2021)
                     /locus_tag="PKL42_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HNU67498.1"
                     /translation="PDLSVEVRARGADWVYAYMRSFYRDETRPSGWNNVVFDKVAMPH
                     VFYELQGEQALDHETHALTLTKPGKLSVEEYDALVGDLTNFMAYMAEPAKQQRNRLGW
                     FVLLFLGVLLVLTYKLKKAYWKDIH"
BASE COUNT          570 a          458 c          412 g          581 t
ORIGIN      
        1 aactcgcgac attcagtttg gaaaactgaa gctctaccaa ctgagctaca cccgcgcatc
       61 catcataact accacagcgc catttggctc ataccgcttt tgcaacacca agtaaacttg
      121 ggcaatctta atattaaata ctttggtgga gggggaagga ttcgaacctt cgtactctga
      181 gagaacggat ttacagtccg tcgcctttaa ccactcggcc acccctccaa accgaacccg
      241 cgattatgct gaaaatttga ctggctgtca aatgtataaa agggtatacg tacgagaatt
      301 tggtgccgga tgtcggaatc gaactgacga ccttcgcatt acaagtgcgc tgctctacca
      361 actgagctaa tccggcgttt aatttgagtt gcgtattata ctgatatttt caaaaatgtt
      421 aagctacttt acgatagtta aggtaggttt tttagttgaa ggcggttttt ctgcattcaa
      481 agccggttca acatccggtt tttgttgagc agaatcatca gaaattggcg ccagatctgg
      541 ctctatttca aaaaacatgc cttggccatt ttctcgcgca aatatgccac gcaccgcatt
      601 tatcggtaca aaaagatttt gtgaaatgcc gccgaacctt gcagaaaaag taattgcatc
      661 atttgttatc tgcaaatctt tggtcgctga gtaatttaaa tttagtacaa tttcaccttc
      721 ttttacatac gcgagcggaa cgcgtgttcg gctattcacc gtaactagca aatggggcgt
      781 acatccgtta tcaacgcacc attcatgaat cgcacgaatc atataaggtt ttgtcgatgt
      841 aaaagctgtc attggtcaat attccttaaa gcgcgttgat gtaaattgtt tttttcggtc
      901 atcaaaatac ttgcaatttt acacaccaaa tctaccgaga aaaaataaat gccctgagag
      961 cctttattta caaggcttgc agggcatcga aaaaggttag cttaaaaaca tggcctttta
     1021 cttaactatt gtttacttac gcatgacctt ttctgatggc gtcatcgcgt ctgcgtaaag
     1081 agggcgcgag aacaaacgct ctgcatattt taatattggt gccgcctgat tttgcagttc
     1141 aattccataa tgcgttaagc gccataataa tggtgtgatt gcaacatcga gcattgagta
     1201 gtcatcgccc agcaaataat tttgtttaga aaaaatcggc acgagttgcg ttaaattatc
     1261 agcaatggtc ttacgtgctt tatctgcaag ttcttcgttg tctgactcaa tatctttaat
     1321 atggtcaaac agctcttttt caaagttata taaaaacaac cttgcgcgag cgcgcatcac
     1381 tgggtcaggc ggcatcaact gcggatgcgg aaaacgctca tcaatgtact cattgataat
     1441 gttggcttct gacaacacca aatcacgttc aaccagcacg ggcacctggt tataggggtt
     1501 aatgactgct aagtcttctg gcttgtttgc caaatccaca tcgatgactt gaaagtccat
     1561 gcctttttca tacaaaacaa tgcggcaacg gtggctgtag ggatcagttg tccctgagta
     1621 taaagtcatc ataatttagt gtatatcctt ccaataagct ttcttgagtt tgtaagtgag
     1681 cactaacaat acgcctagaa acagcaatac aaaccaaccc aagcgattac gttgctgctt
     1741 tgctggctcg gccatatagg ccataaagtt tgttaaatcc cccaccaagg catcatattc
     1801 ttcaaccgac aatttgccgg gctttgtgag ggttaaggcg tgcgtttcat gatcaagcgc
     1861 ttgctcacct tgcaattcgt agaatacatg cggcatcgcc actttatcaa acaccacgtt
     1921 gttccaccct gacgggcgtg tttcatcacg gtaaaagctg cgcatatagg cataaaccca
     1981 atcagcgccg cgtgcacgta cttctacaga caaatcaggt g
//
LOCUS       DAOQSI010000233         2015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_71102_length_2015_cov_6.254592, whole genome shotgun sequence.
ACCESSION   DAOQSI010000233 DAOQSI010000000
VERSION     DAOQSI010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71102_length_2015_cov_6.254592"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..768
                     /locus_tag="PKL42_10280"
     CDS             223..768
                     /locus_tag="PKL42_10280"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNU67499.1"
                     /translation="MTLKLLFYVVVLIIAVIGYRSYSTVPKTLKLGQNAPPFLLKDAN
                     GKTHQLTDYEGKYLVLYFYPKDDTPGCTEEACQFRDDFVQLEKLGAKVVGVSVDSSES
                     HANFAKKYHLPFPLLADTDGKVAESYYALSNFVVLKIAKRYTFLINPTSKVVKVYTSV
                     NTSKHSQQIINDLKAMQISPS"
     gene            804..1850
                     /locus_tag="PKL42_10285"
     CDS             804..1850
                     /locus_tag="PKL42_10285"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNU67500.1"
                     /translation="MHFNINAYLMIGIFITLLTVTGLSFAYALKVKRNEALWKFALES
                     TGDGVWDWDIQNNQARFSNSYQEMFGFNVDEINANLQNWYDRIHPDDLASKKEAVNNH
                     LIGKIEKYIHEHRVICKDNSVKWVLSRGMVVERDKAGNPLRMVGTHVDITERKRNEHK
                     HSNLAHLDALTNLPNRKLFNDRLKLALASAKRSKKMLAVLFIDLDLFKEINDQHGHKV
                     GDMVLKKTAQRLLSCVRETDTVARLGGDEFVILISNIESESDVHKITDKLLESAVKPI
                     EIVKSNDTSQIIDLKLRNLQVSASIGVAIYPQHGTNEKTLVTNADSAMYLAKKTGKNQ
                     AKFFDDQTIVTYDI"
     gene            complement(1920..2006)
                     /locus_tag="PKL42_10290"
     tRNA            complement(1920..2006)
                     /locus_tag="PKL42_10290"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1970..1972),aa:Leu,seq:taa)
BASE COUNT          634 a          409 c          424 g          548 t
ORIGIN      
        1 aacgtgtgac cctcccctta ggagggggat gctctatcca ctgagctacg ggggctaatg
       61 aagggcgtat tttctcatat ttcaacgcag ttagcttgcg tgtttaatac cgctagcaca
      121 accaaaattg caatagtgag caaaatcaaa tggtgctaac atatgcatca gcttgtactt
      181 tttacctcat tcatttttta ttatcgcaaa gcaattacac tgatgacact taaattatta
      241 ttttatgtag tcgtgttaat tatcgccgtt attggttatc gtagctattc gactgtgcca
      301 aaaacactaa agcttggcca aaatgcaccg ccttttttat taaaagatgc aaacggaaaa
      361 acgcaccaac taactgatta tgaaggaaaa tatttagttc tttactttta ccccaaagat
      421 gacacgccag gttgcaccga agaagcctgc caatttagag atgatttcgt gcaacttgaa
      481 aagctaggcg caaaggttgt gggcgtcagt gttgatagca gtgagtctca tgcgaacttt
      541 gccaagaaat atcatctgcc ttttccgctt ttggccgata ctgacggaaa agtcgctgag
      601 agttattatg cattgagtaa ctttgttgtg ttaaaaattg ccaaacggta tacttttctc
      661 attaacccta ctagtaaagt agttaaagtg tatacaagcg tgaatacgtc aaaacactca
      721 cagcaaatta ttaatgacct gaaagcgatg caaattagtc cctcataaaa tatcggctat
      781 tgcactttta aaaaagaata aaaatgcatt tcaatataaa cgcttacctc atgattggta
      841 ttttcattac cctgctcacc gtgacaggct tgagttttgc ctacgcactg aaagtgaaaa
      901 gaaatgaagc gctttggaag ttcgccttgg aaagtaccgg tgatggcgtt tgggattggg
      961 atattcaaaa taaccaagcg cgcttttcaa acagttatca agaaatgttt gggtttaatg
     1021 tggatgaaat caatgcaaat ttacaaaact ggtacgatcg cattcatcct gatgacctcg
     1081 catcaaaaaa agaagccgtt aataaccacc taatcgggaa aattgaaaag tacattcacg
     1141 agcatcgcgt gatctgcaaa gataacagtg tgaaatgggt gctttcacgc ggcatggtgg
     1201 tagagcgcga taaagcaggc aatccactgc gtatggttgg cacgcatgtg gacattacgg
     1261 aacgcaaacg caatgagcac aaacatagta accttgcgca tttagacgcg ctcaccaatc
     1321 tacctaacag aaaattattt aacgaccgac taaaactggc gctcgcaagc gccaaacgca
     1381 gcaaaaaaat gcttgcggtg ttgtttatag accttgattt atttaaagaa atcaacgacc
     1441 agcatgggca taaagtaggt gacatggtgc ttaaaaaaac cgcccaacgg cttctttcct
     1501 gcgtgcgtga gactgatacg gttgctcgct taggtggtga tgaatttgtg attttaatct
     1561 ctaacattga gagcgaatca gatgttcata aaatcaccga caagctgctg gagtctgccg
     1621 ttaaacccat cgaaatcgtg aaatccaacg atacttcgca gattatcgac ttaaaactca
     1681 gaaacttaca agtgtcggcc agtataggtg tagccatcta tccgcaacac ggaacgaatg
     1741 aaaaaacgct cgtcacaaat gccgatagcg caatgtattt agcgaaaaaa actggaaaga
     1801 atcaagcaaa gttttttgat gaccaaacca ttgtaaccta cgatatttag cgactaagtc
     1861 tggcctaaac agctttatgg ctgggataaa aagttttgtt ttcttgatgt gttgatttat
     1921 ggtgcccggg gccggaatcg aaccggcacg accttacgat cgagagattt taagtctctt
     1981 gtgtctacca atttcaccac ccgggctaag ggtga
//
LOCUS       DAOQSI010000234         1890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_78908_length_1890_cov_4.257766, whole genome shotgun sequence.
ACCESSION   DAOQSI010000234 DAOQSI010000000
VERSION     DAOQSI010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78908_length_1890_cov_4.257766"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PKL42_10295"
     CDS             <1..300
                     /locus_tag="PKL42_10295"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNU67501.1"
                     /translation="SIGAMEKGSSDRYFQDNNGNADKLVPEGIEGRVPYKGSVIAVIH
                     QLMGGLRASMGYVGCATIDEMRNKAEFVQITSAGMRESHVHDVQITKEAPNYRVD"
     gene            393..>1890
                     /gene="guaA"
                     /locus_tag="PKL42_10300"
     CDS             393..>1890
                     /gene="guaA"
                     /locus_tag="PKL42_10300"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNU67502.1"
                     /translation="MTHSKILILDFGSQVTQLIARRVREAHVYCEIHPCDVSNEFVKN
                     FGANGIILSGSHASAYEEDTDKAPQAVFELGVPVLGICYGMQTMAQQLGGKVEAGTKR
                     EFGYAEIRARGHSALFKDIQDRTNTEGHGLLDVWMSHGDKVTELPPNFKVIASNDTTP
                     IAAFADETRKFYGVQFHPEVTHTKLGQAMLERFVLEICQAKPDWVMKDHIAEAVEKIK
                     AQVNDEEVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGDMVMDMFVGK
                     LHANVIRVDAADQFMGHLAGVSEPEAKRKIIGREFVEVFKAEAAKLKAGNSGHKGAKW
                     LAQGTIYPDVIESGGAKSKKAVTIKSHHNVGGLPETLGLKLLEPLRDLFKDEVRELGV
                     ALGLPHEMVYRHPFPGPGLGVRILGEVKREYALLLQRADAIFIEELRNTVDEKTGKTW
                     YDLTSQAFTVFLPVKSVGVMGDGRTYDYVVALRAVVTSDFMTAHWAELP"
BASE COUNT          512 a          425 c          476 g          477 t
ORIGIN      
        1 tcaattggcg caatggaaaa aggctcgagc gaccgttact tccaagacaa caatggcaat
       61 gccgacaagc tcgttccgga aggcattgaa ggccgtgtgc cttacaaagg cagtgtgatt
      121 gccgtgattc accagttaat gggtggtttg agggcgtcta tgggctacgt tggttgcgcg
      181 acgattgatg aaatgcgcaa caaagctgag tttgtgcaaa tcaccagtgc aggcatgcgt
      241 gaatcacacg tgcatgatgt acagattacc aaagaagcgc caaactaccg cgttgactaa
      301 ttgccgtgaa aggtgaaaag ggaaatgtga aacgttaccc ttattcaacc tttcacgttt
      361 cacgtttcac gtttcatttt tcacctaact ttatgaccca ttcaaaaatc ctcattctcg
      421 atttcggctc acaagtcacc caactgattg cccgtcgtgt gcgcgaagcg catgtatatt
      481 gcgaaataca tccatgcgat gtttctaacg aatttgtgaa aaactttggc gcaaatggca
      541 ttattttatc gggcagccac gccagtgctt atgaagaaga cactgacaaa gcgccacaag
      601 cggtgtttga gctaggcgtg ccagttttag gcatttgtta cggtatgcaa accatggcgc
      661 agcaattagg cggcaaagtt gaagctggca caaaacgtga gtttggctac gcagaaatcc
      721 gcgcacgtgg acactcagct ttatttaaag acattcaaga ccgcaccaat actgaaggcc
      781 atggcctgct ggacgtttgg atgagccacg gtgataaagt gactgaatta ccacctaact
      841 ttaaagtgat tgccagcaac gacaccacac ccattgccgc gtttgcggat gaaacacgca
      901 aattttacgg tgtgcagttt cacccggaag tgacgcacac aaaacttggg caagccatgt
      961 tagagcgctt tgtattggag atttgccaag ccaaacctga ttgggtgatg aaagaccata
     1021 ttgctgaagc ggtagaaaaa attaaggctc aagtaaatga cgaagaagtc attttaggtt
     1081 tatctggtgg cgtcgattct tctgttgccg cagccctcat ccaccgcgcc attggcgacc
     1141 aactcacctg tgtttttgtg gaccatggtt tattgcgcct gaatgaaggc gacatggtga
     1201 tggatatgtt tgttggcaaa ctgcacgcaa atgtcattcg cgtagatgcc gctgaccaat
     1261 ttatggggca tttagccggc gtgagtgaac ctgaagccaa acgtaaaatt attggccgcg
     1321 aatttgtaga agtgtttaaa gcagaggccg ccaagctcaa agctggcaac agcgggcata
     1381 aaggtgcaaa atggctcgcg caaggtacta tttaccccga tgtcattgaa tctggcggtg
     1441 caaaaagcaa aaaagcagtc accattaaaa gccaccataa tgtaggcggc ttgcctgaaa
     1501 ccttgggttt aaaattactt gagccgttgc gtgatttatt taaagacgaa gtgcgtgagc
     1561 ttggcgtggc gcttggactc ccacatgaaa tggtctatcg tcaccctttc ccaggcccag
     1621 ggttgggcgt gcgtatttta ggcgaagtca aacgtgaata cgccctgctc ttgcaacgtg
     1681 ctgatgctat ttttattgaa gaactacgca atactgttga tgaaaaaacg ggtaaaactt
     1741 ggtacgacct gactagtcag gcttttacgg ttttcttacc agtaaaatct gttggcgtca
     1801 tgggcgatgg ccgcacttat gattacgtgg tagccttgcg cgccgtggtc accagtgact
     1861 tcatgaccgc acattgggca gaattaccct
//
LOCUS       DAOQSI010000235         1878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_79675_length_1878_cov_5.306089, whole genome shotgun sequence.
ACCESSION   DAOQSI010000235 DAOQSI010000000
VERSION     DAOQSI010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79675_length_1878_cov_5.306089"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1857
                     /locus_tag="PKL42_10305"
     CDS             <1..1857
                     /locus_tag="PKL42_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HNU67503.1"
                     /translation="IEAINHGADAVYIGGPAFGARAKAPNTLEDIARLVKHARRFHAE
                     IFVALNTIFHDNELEAAQRLTHQLYEIGVDALIIQDMGLLELDLPPIQLHASTQTDIR
                     TVEKAAFLDQVGFSQIVLARELDLKTVKQIAEATSANLEYFIHGALCVAFSGQCYISH
                     AHTGRSANRGECSQECRLPFTLEDQKGRIIGKDKHYLSMKDNDQSANLRALIDAGVSS
                     FKIEGRYKDLPYVKNATAHYRQLLDEILHDRPELAKSSHGNCTYTFNPQPEKTFNRSA
                     TDYFANGRQADIGAFDTPKFSGELLGKVTKVGKDFIEVETPTQLHNGDGVCFFDVHKE
                     LVGLRINTVDGKKLYPNEMPADIRNNTIVYRNRDHAFMRLLEKDSAQRKIRLNLRFFE
                     TSDGFGLEVIDEAGINVTYLTHAEKQVAQTPDKAIHSLKENLSKLGSTDFVVNSIEIA
                     TTQTWFVPASIANALRREAIDGLQIARNKSYARPVRRMAAKPPAIYPEDTLSYLANVY
                     NQKARQFYAKHGVNLIAKAYEANKELGEVPVMITKHCLRFSHGLCPKEAKGVIGVQGT
                     VTAEPMTLISGNDRFTLKFDCKPCEMHVMGKIRKPVIQMGPPQTVQFFNKRP"
BASE COUNT          560 a          428 c          437 g          453 t
ORIGIN      
        1 attgaggcca ttaaccatgg ggcggatgca gtgtatattg gcgggccggc gtttggcgcg
       61 cgggccaaag cgccaaatac attagaagat attgcgcgtt tggtaaaaca tgcgcggcgt
      121 tttcatgccg aaatttttgt tgcgctcaac accatttttc atgataacga gttggaagcc
      181 gcgcaaagac tcacacatca actctacgaa attggcgtgg acgcactgat tattcaggat
      241 atgggtttgc tagaactaga cttgccgccc atacaactgc acgccagcac gcaaacggat
      301 attcgcacgg ttgaaaaagc cgcgttttta gaccaagtgg gctttagcca aatagtgtta
      361 gcgcgcgagc tagatttaaa aacggtcaag caaattgccg aagccaccag cgcaaattta
      421 gaatatttta ttcacggggc attgtgtgtg gcgtttagcg gacaatgtta tatcagccac
      481 gcccataccg ggcgaagtgc caaccgtggt gagtgttcgc aagaatgccg attacctttt
      541 acattagaag atcaaaaagg ccgcattatt ggcaaagata agcattatct atcaatgaaa
      601 gataatgacc aaagcgccaa cttgcgcgcg ttaattgatg cgggcgtcag cagctttaag
      661 attgaaggtc gctataaaga tttaccttat gtcaaaaatg cgactgcaca ttatcgccaa
      721 ctattagatg aaattttgca cgataggcca gaattagcca aatcatcaca cggcaattgc
      781 acttacacct ttaacccaca gcccgaaaaa acctttaacc gcagcgcgac ggattacttt
      841 gccaacggtc gccaagccga tattggtgcg tttgatacgc ctaaattttc aggcgaattg
      901 ttaggaaaag taactaaggt aggcaaagat tttattgaag tggaaacgcc cactcagtta
      961 cataatggcg atggcgtgtg cttttttgat gtgcataaag aattagtggg cttgcgcatt
     1021 aacaccgtag atggtaaaaa gctttacccc aacgagatgc ccgctgatat tcgcaacaac
     1081 acaattgtgt atcgcaaccg cgaccatgct tttatgcgat tgcttgaaaa agattctgca
     1141 cagcgcaaaa tcaggctcaa cttgcgtttt tttgaaacca gcgatggctt tgggctagag
     1201 gttatcgacg aagctggaat caacgtcact tatttgacgc atgcagaaaa gcaagtagcg
     1261 caaacgcccg ataaagccat ccatagccta aaagaaaatt taagtaaact tggtagcact
     1321 gattttgttg tgaattccat tgaaatcgcg accacgcaaa cctggtttgt acccgcatcg
     1381 attgccaacg cacttcgccg tgaggcaatt gatggcttgc aaatcgcgcg taataaaagt
     1441 tatgcgcggc cagtacgtcg tatggctgcc aaaccacccg cgatttatcc tgaagataca
     1501 ttaagttacc tcgccaacgt ctacaaccaa aaagcgcggc agttttatgc aaaacacggc
     1561 gtaaacctca ttgcaaaagc ctatgaggcc aataaagagc tcggtgaagt gccggtgatg
     1621 attaccaaac attgcctgcg ttttagccac gggctatgcc ccaaagaagc caaaggcgtg
     1681 attggcgtgc aaggcacggt gacggcagaa cccatgacgc tcattagcgg caacgaccga
     1741 ttcacattga aatttgactg taagccctgc gagatgcatg tgatggggaa aatccgtaag
     1801 cccgttattc aaatgggccc gccacagact gtacaatttt ttaataaacg accttaaaaa
     1861 aactacattt ttaaagcg
//
LOCUS       DAOQSI010000236         1702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_93688_length_1702_cov_3.335155, whole genome shotgun sequence.
ACCESSION   DAOQSI010000236 DAOQSI010000000
VERSION     DAOQSI010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93688_length_1702_cov_3.335155"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="PKL42_10310"
     CDS             <1..1060
                     /locus_tag="PKL42_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNU67504.1"
                     /translation="CPCALGLATPMSIMVATGKAASQGVLFRDAAAIENFRKIDTIIV
                     DKTGTLTEGKPKFDRVIANDAYTEDEVLRLAASLDQGSEHPLADAIVQAAREKGLVLD
                     KVEAFDSISGIGVRGNVTGKTLALGNTALMTQIGVSVEDLKPQAEALRAEGASVMILA
                     VDGKMAGVLAVSDPIKASSLEALSILRSFDIRVIMATGDGLATAKSVAAKLGIDEVYG
                     EVRPADKLALVDKLQREGRFVAMAGDGINDAPALAKANVGVAMGTGTDVAMNSAQVTL
                     VKGDLRGIAQARAISEQTIINMKQNLIFAFVYNSLGVPLAAGLLYPLTGWLLSPMIAA
                     LAMSLSSVSVITNALRLR"
BASE COUNT          505 a          344 c          390 g          463 t
ORIGIN      
        1 ctgcccatgt gcactagggc tggcaacgcc gatgtctatt atggttgcta caggaaaggc
       61 tgcttctcaa ggagtgttat tccgtgatgc ggcagccata gagaatttcc gtaaaattga
      121 caccatcatt gtcgataaga cagggacatt gacagaagga aagcctaaat ttgaccgagt
      181 tattgccaac gatgcgtata ccgaagatga agtgttacgt ctggcagcca gtttagatca
      241 gggaagtgaa caccctcttg cagatgctat tgtgcaagca gcccgtgaaa agggacttgt
      301 acttgataaa gtggaagcct ttgattcaat ctctggtatc ggcgttaggg gaaatgtgac
      361 tggtaaaaca cttgcattag gcaatactgc actgatgacg caaattggtg tatctgtaga
      421 agatttgaag ccacaggcag aagcattaag agccgagggg gctagtgtaa tgatattggc
      481 ggtagatggg aaaatggccg gagtactagc tgtttctgac ccaattaagg caagttcatt
      541 agaagcattg agcattctca gatcgtttga tattcgggta atcatggcaa ccggagatgg
      601 tcttgcaaca gcaaaatcag tagctgccaa acttggtatt gatgaggtct atggcgaagt
      661 caggcctgct gacaaattag cgctggtaga taaattacaa cgtgagggcc gttttgttgc
      721 aatggcgggc gatggtatca acgatgcacc tgcgctggct aaagcgaatg taggtgttgc
      781 aatggggact gggacagacg ttgcaatgaa tagtgcccaa gtaacgctcg taaaaggtga
      841 cctgcgcggc attgctcaag ctagagcaat ttcagagcag actatcatta atatgaaaca
      901 aaatcttatt tttgccttcg tatataattc actaggtgta cctttggctg caggtctact
      961 ttatccactt actggatggt tattatcacc gatgattgct gctctcgcca tgagcttaag
     1021 ttcagtatct gtgattacaa atgcattacg tttgaggtaa tacaattatt taattaatat
     1081 ctagtgtatt ttgaacgtct gctttggatg gtctatagag atcaactcac aaaaatggaa
     1141 tagccgcttt gtcccctaac tactctacca ctcagccagc caaaccagcg ctccatcact
     1201 cagtacccat taaaaatgca cagattacca acgtccactc atgcaaattt aaataaaaaa
     1261 aatccagatt gcaccgccaa aattttgcat aaaactttcg cagcagcacc ctaaaatttg
     1321 ccgccacgcc gcaacatttc tcgcacagcc aattacggca aaggttttag cgttgcggat
     1381 aaaaagaaac gcatcctaaa cgaagtttcc agtccgccac attcgactca cacgtttcgc
     1441 cacacaaata tatctgcaac atacccgaaa tgcggagagt gatgctaggc atcgaagttg
     1501 cggcgttgcg gtaatttcag gaggtaggta tgtgagtgac tttctaacac ttatttgata
     1561 tttatctaaa aattaggttg acatcgtgaa atttcattat tattatttgg cgtattgtag
     1621 ataaagcgat ttaaataaat cgagtttgca atatgtctta agagctgaaa gcaatcgtct
     1681 ttctgcatat gaaacctagc at
//
LOCUS       DAOQSI010000237         1621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_101640_length_1621_cov_4.314176, whole genome shotgun
            sequence.
ACCESSION   DAOQSI010000237 DAOQSI010000000
VERSION     DAOQSI010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101640_length_1621_cov_4.314176"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..599)
                     /locus_tag="PKL42_10315"
     CDS             complement(24..599)
                     /locus_tag="PKL42_10315"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HNU67505.1"
                     /translation="MNSILFLILRRLRRPLILIIVSFGIAVLGLTLMPGLDDKGQPWH
                     MSVFQALYVISYTATTIGFGEIPYPFSQAQRLWTTFSIYFTVIPWFYAIGKIITLLQD
                     SALKQAIASERFSRAVALLQEPFYILCSYGQSATLLAKALDHMYMRVVVIERSQNRLD
                     ELELTDSKQVIPHFCGITCLLSVSSSSSRRF"
     gene            complement(646..1020)
                     /locus_tag="PKL42_10320"
     CDS             complement(646..1020)
                     /locus_tag="PKL42_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6394 family protein"
                     /protein_id="HNU67506.1"
                     /translation="MNLEKVIFGFFIVLAMTLNFGFFIGDIDNPSHHHVYELYAAIVV
                     SLIATVLKFGDRTHVGAILLSTSLAADLQLITAAILWAALHGKYDEPALMVYIVSLSG
                     GALMANIVSVVLLIAETISQRR"
     gene            1162..>1621
                     /gene="miaA"
                     /locus_tag="PKL42_10325"
     CDS             1162..>1621
                     /gene="miaA"
                     /locus_tag="PKL42_10325"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNU67507.1"
                     /translation="MQTTANPPAIFIMGPTASGKTGAAVALCERLPIEIISVDSALVF
                     KEMNIGTAKPDAATLEKAPHHLIDLILPTSAYSAANFRTDALRLMAEITGRGKIPLLV
                     GGTMLYFKALQEGLTGLPEANPTIRAKLDARAALIGWPAMHEKLSLIDPIT"
BASE COUNT          514 a          349 c          348 g          410 t
ORIGIN      
        1 tcacttgttt gctatcggtg agttcaaaat cgcctggacg aacttgaact caccgatagc
       61 aaacaagtga ttccacaaaa atgtggaatc acttgtttgc tatcggtgag ttcaagttcg
      121 tccaggcgat tttgcgagcg ctcaatcacc acgacacgca tgtacatatg gtctaatgct
      181 ttggctaata aggttgcaga ctgaccatag ctacataaaa tataaaaagg ctcttgtaac
      241 aaagcaactg ctcggctaaa acgttcagaa gcgatggctt gcttgagtgc gctatcctgc
      301 aataaggtga taattttacc tatggcataa aaccaaggaa tcacggtgaa gtagatagaa
      361 aatgtagtcc acaaacgctg tgcctggcta aatggataag gtatttcacc aaaaccgatg
      421 gtggtagcgg tgtagctgat aacataaagc gcctgaaaca cgctcatgtg ccatggttga
      481 cctttatcat ctagcccggg catgagcgtc aaccccagca ccgcaatacc aaaactcaca
      541 ataattaaaa tgagcggtct gcgcaaacgg cgcaaaatca aaaacaggat gctgttcatc
      601 aggcttaaac tttagactta aatttaaata tgtgcgtcgc taaatttagc gtctttgact
      661 aatggtttcg gcaataagta ataccacaga gacgatgttg gccattaagg cgccgccaga
      721 taatgacacg atatacacca ttaaagcagg ttcgtcatat ttgccgtgta aagcggccca
      781 caaaatggca gcggtaatca gttgtaagtc tgcggctaaa ctggtggaga gtaaaattgc
      841 gccaacatgt gtgcgatcac caaatttaag cacggttgca ataaggctca caacaatagc
      901 ggcataaagc tcgtatacat gatgatgtga cggattgtcg atatcaccaa taaaaaatcc
      961 aaaattgagt gtcatcgcca atacgatgaa aaaaccaaaa ataacttttt ctaaattcat
     1021 atctctgtcc gtgaaaagaa gaaaggtgcg tgaggaataa gtagtggcac aataatggtt
     1081 tattatacaa gcctcctagc cagataaaat aaagcctagc attttttatt ggttaattta
     1141 catactattc aatatttcaa catgcaaaca acagccaatc ccccagcaat ttttataatg
     1201 ggcccaactg ccagtggaaa aacgggtgcg gcagtggcac tctgtgagcg actacctata
     1261 gaaattatta gtgttgattc cgcgcttgta tttaaagaga tgaatatcgg cacggccaag
     1321 ccagatgctg cgacgcttga gaaagcgccc caccatctta ttgatttaat tttacctaca
     1381 agcgcctatt cagcagcaaa ctttagaacg gatgccttac gtttaatggc agaaatcact
     1441 gggcgaggca aaatcccatt gctagttggc ggcacgatgc tttattttaa agcgctgcaa
     1501 gaaggtttaa ccggcttacc tgaagctaac ccgacaatac gtgccaaact tgatgcgcgg
     1561 gctgccctga ttggctggcc tgccatgcat gaaaaattat ccttgattga tccaatcact
     1621 g
//
LOCUS       DAOQSI010000238         1612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_102574_length_1612_cov_4.785485, whole genome shotgun
            sequence.
ACCESSION   DAOQSI010000238 DAOQSI010000000
VERSION     DAOQSI010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102574_length_1612_cov_4.785485"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PKL42_10330"
     CDS             <1..450
                     /locus_tag="PKL42_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNU67508.1"
                     /translation="PVEMLFDQALGDIFSVRLAGNIASDKVIGSIEFSSKYLGSKLIV
                     VLGHTDCGAVKAACDNFREGHIGEIINLIRPAVRLEKTIEAQRDSSNVNFVDKVCALN
                     VQVQINEIVHSSDIIQDMLAAKQIGIVGGVYNLATGEVRFLPETFML"
     gene            687..>1612
                     /locus_tag="PKL42_10335"
     CDS             687..>1612
                     /locus_tag="PKL42_10335"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na-translocating system protein MpsB"
                     /protein_id="HNU67509.1"
                     /translation="MKSHIFDEQHILAELKHYLPSQSPLKDFIHHNSLHAFQNIKFYD
                     AIFKASKIFGFQATTPLTDFRAMHANGRIKDEVLNRVIAERFGVAAQALWRQKAVEQQ
                     YDEHNQARIGQLRARWKQRYQVNLDDAVQPLLFRLLSHYLDQGIAIWAFPDTQQGLLA
                     SVRHLESASYVSLFKSSRARQLLAEVDLSITKLLNILVGDEAYFSQYLFDQQFGHRGW
                     SGMVATIEANPSVLLDEKKITVAELIILELLLEIDAMDDALGTAWQPIAHASDLSPVD
                     LFAKQPYSELADVLKIWQDAFEWSYYDEVLAG"
BASE COUNT          462 a          307 c          389 g          454 t
ORIGIN      
        1 ccggttgaaa tgctgtttga ccaagcgttg ggggatattt tcagtgtacg tttggcgggc
       61 aatattgctt cagacaaagt gattggtagt attgaattta gctcaaagta tttgggcagc
      121 aagctgattg tggtgctagg gcataccgat tgtggcgcgg taaaagcagc gtgtgataat
      181 tttagagaag gccatattgg cgaaattatc aacttaattc gcccagcagt gcgcttggaa
      241 aaaaccatag aagcacagcg agattcaagc aatgttaatt ttgtcgataa agtttgcgct
      301 ttaaatgtgc aagtgcaaat caatgaaatc gtccatagca gcgatattat tcaagatatg
      361 ctggccgcaa aacaaattgg tattgtgggc ggtgtttata atttagccac gggtgaagtg
      421 cgttttttgc ctgaaacttt tatgctgtaa ataacacgta attaaacgaa ttttgaacgg
      481 tattttctct cgagagaaaa aatatgctct ggaagccttg taaataaagg cttcaagagc
      541 atcgaaaaag gtttcgtatg caaatgcact ggttacatgc ttttttaatg aagcgtgtaa
      601 attgttgcaa atttagtgac gcaacgcagt actagcgtaa gtgttcagct gccatcaggc
      661 ggcatttttt tgtacagggt aggcacatga aaagccatat ttttgacgag cagcacattc
      721 tagctgagct taagcattat ctgccatccc agtcgccctt aaaagacttt atacatcata
      781 actctctgca cgcatttcaa aacatcaagt tttatgatgc tatatttaaa gcctctaaaa
      841 tattcgggtt tcaagccacc acaccactga cagattttag agccatgcac gcaaacgggc
      901 gtattaaaga tgaagtgctc aatcgtgtga tagcagagcg ctttggtgta gcggcgcaag
      961 cattatggcg ccaaaaagcg gttgagcaac agtatgatga acataatcaa gcccgtattg
     1021 gtcagctaag agcgcgttgg aagcaacgtt atcaagtcaa tttagatgat gcggtgcaac
     1081 ccttattatt tcgtttattg agtcactatt tagatcaggg catcgcaata tgggcgtttc
     1141 cagacacgca gcaggggttg ctcgccagcg tcaggcatct tgaaagcgcg agttatgtga
     1201 gcttattcaa atcatcgcgg gcgcgtcagc tattggctga agtagattta tccatcacaa
     1261 aattactcaa tatactcgta ggtgatgagg cgtatttttc gcaatatcta tttgaccagc
     1321 agtttgggca tcgtggttgg agtggtatgg tggcaaccat agaagccaac ccttcggtgc
     1381 tgctagatga gaaaaaaata acggtcgcag agctgattat tttggaacta ttgctagaaa
     1441 ttgatgcaat ggacgacgcc cttggtacag catggcaacc catcgcgcac gcgtcagacc
     1501 tttcacccgt tgatttgttt gccaaacagc cttacagcga gctcgcagat gtcctgaaaa
     1561 tttggcaaga tgcatttgaa tggagttatt acgatgaagt cttggcgggt at
//
LOCUS       DAOQSI010000239         1513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Methylotenera sp. isolate AMR_MDS_1170
            NODE_114047_length_1513_cov_4.955418, whole genome shotgun
            sequence.
ACCESSION   DAOQSI010000239 DAOQSI010000000
VERSION     DAOQSI010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852513
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylotenera sp. (wastewater metagenome)
  ORGANISM  Methylotenera sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae; Methylotenera.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:56:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,068
            CDSs (total)                      :: 2,046
            Genes (coding)                    :: 2,036
            CDSs (with protein)               :: 2,036
            Genes (RNA)                       :: 22
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 7 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Methylotenera sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114047_length_1513_cov_4.955418"
                     /isolate="AMR_MDS_1170"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2051956"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            10..>1513
                     /locus_tag="PKL42_10340"
     CDS             10..>1513
                     /locus_tag="PKL42_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HNU67510.1"
                     /translation="MWILFATFVGFSHAEDQSMLANKIESVDFSTLPGGRVSIRIKTT
                     EPLVNAPAGFTLSDPARIALDFPKVGNGLAKNNITAGQGELKSITLAQAKDRTRMVLN
                     LTKNVGYTTSLSGNELTVMLQANAAGAATVVETKFSEPALGSQQNAISNVDFARGKNG
                     EGRIIVDLSDPNSAINIKQKGKTITVDFVNTDVPANLQRRLNVTNFNTPVIYVDTMKQ
                     GNNGRMVIEPKGNWEHSAYQADKKFIIDVRQVVEDPNKLVRGNKPGYNGEKLSLNFQN
                     IEVRSVLQVIADFTGLNIITSDTVSGNLTLRLKDVPWDQALDIITQSKGLAMRKTGNV
                     IIVAPAEELAAKEKQALEAIQQIDDLEPLRTEVFTLKYMKAESLTNILSDPKQKILSK
                     RGSAVLDPRTNTVFVQDTPKYLEQVQAIINRTDVPVRQVMIESRLVIADDKYGKALGA
                     RFGVTQTSTPGRSSSTISGSMGNRPTSFSGGTLTQGNHNGAMQTALTDQYL"
BASE COUNT          484 a          285 c          357 g          387 t
ORIGIN      
        1 catgcatgca tgtggatttt atttgcaact tttgttggat tttctcatgc agaagatcaa
       61 tctatgcttg caaataaaat tgaaagtgtg gatttttcaa cgttgcccgg aggtcgagtc
      121 agcattcgca ttaaaacaac ggaaccgctg gtgaatgctc cagcaggttt tacactaagc
      181 gacccagcac ggattgcgct agattttcct aaagttggga acggtcttgc gaaaaataat
      241 atcacagctg gccaagggga actgaaaagt atcactctgg cacaagctaa agatcgcacg
      301 agaatggtgt tgaatttaac aaaaaatgta ggatatacaa caagtttgag tggcaacgaa
      361 ttaaccgtga tgttgcaagc caatgctgca ggcgcagcca cagttgtaga aactaaattc
      421 tctgagcctg cactgggtag ccagcaaaat gccatcagca atgttgattt tgcacgtggt
      481 aaaaatggcg aaggtcgaat cattgtagac ttgtctgatc caaactcagc aatcaatatc
      541 aaacagaagg gtaagacaat tactgtagac tttgttaata cggacgttcc agccaatttg
      601 cagcgtcgtt taaacgtgac aaactttaac actcccgtta tttatgttga tacgatgaaa
      661 caaggcaata atggacgtat ggtaattgag cccaaaggca attgggagca ttcagcctat
      721 caggcggata agaaatttat tatcgacgtg cgtcaggtgg tagaagatcc gaataaactt
      781 gtgcgtggta ataaacctgg gtacaacggt gagaaattat cattaaattt ccagaatatt
      841 gaagtacgct cagttttaca ggtaatcgca gactttactg ggctaaatat tattacgagt
      901 gacactgtat ctggcaactt aacgctacgc ttaaaagatg tgccatggga ccaagcgcta
      961 gatatcatta cgcaaagtaa gggtttagcg atgcgtaaaa caggcaatgt gattattgtg
     1021 gcaccagctg aagagttggc agcgaaggaa aagcaggcgc ttgaagctat tcagcagata
     1081 gatgatttag agccgttacg cacagaagtg tttacactca aatatatgaa agcggagtct
     1141 ttgacaaata tattaagtga cccaaagcag aaaattttgt caaaacgtgg gagtgcggtg
     1201 cttgaccccc gtactaacac agtatttgtg caggacacac ctaaatatct tgagcaagta
     1261 caagctatca ttaatagaac agatgtacca gttaggcagg taatgattga gtcacgctta
     1321 gtgattgcgg atgataaata tggcaaagcg cttggcgcaa ggttcggcgt aacgcaaaca
     1381 tctacaccag gcagaagttc aagtactatt agtggctcaa tgggtaatag gccgacatcc
     1441 ttttcaggtg gaacgttaac tcagggtaat cacaatggcg ctatgcaaac ggctcttaca
     1501 gatcagtacc ttg
//
